LOCUS       DAORPW010000001        60387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_57_length_60387_cov_770.570178, whole genome shotgun sequence.
ACCESSION   DAORPW010000001 DAORPW010000000
VERSION     DAORPW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 60387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60387
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57_length_60387_cov_770.570178"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1445)
                     /locus_tag="PLB86_00005"
     CDS             complement(<1..>1445)
                     /locus_tag="PLB86_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HRC63550.1"
                     /translation="VLAVAADAADLPSACTQIVDLRAGTPSLTWAQQPVTGLEPDGVP
                     RAWALLLAQRLGPLRDAAATPHKGVIPSRTTLLDVLEIPERDGIPDLTARWRESPRST
                     LTPIGATASGPLPIDLVRDGPHALVGGTTGSGKSELLVSLVAGLAAANRPDELSFVLV
                     DYKGGAAFGACRDLPHVVGLVTDLDHQLTERALVSLDAELKRRERVFGEHGVADLAAY
                     QGVCRPGDPTVGRLVIVVDEFRSLAEELPDFVTGLVRVASLGRSLGVHLVIATQRPGG
                     VVTADMRANLGLRIALRVRDIVDSLDVVESPVAASIAESTPGRAILTSAATRLVEVQT
                     ALATGPSRRAVEPPVRVVSIDGLAVPQSVRALDGPTQVDDLVAASRRAVLELSIPLPG
                     SPWLPPLSGDLTVASLAGGSGWPVPLGRRDVPLHQRQSDWTWDPLHEGHLGIVGAPRT
                     GRTTALLTVAGQLAARFSPDEIHLYAVHAGSL"
     assembly_gap    1446..1545
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1546..2531)
                     /locus_tag="PLB86_00010"
     CDS             complement(<1546..2531)
                     /locus_tag="PLB86_00010"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRC63551.1"
                     /translation="MAGRAVEVRVAARRGATFAELLDALDAAHALTDTSRTWSVDSVG
                     VPPTATLGLPPLLDGARLMIRPAGPTSPDTSTASTASGLLQLHAVGGPDTGLVHDLPP
                     GRHVLGRGANTPIRVGDSSLSRLHAEITVAGRSITVRDLESTNGTWVNGIPVGGEPIT
                     LAAGATLQTGDSRWQLRTPATVSAATRPDHEGHLLVSRSPRLPLPGPAAPIPFPEEPN
                     PPAGQRLPIAAATLPLVFSAVLAVVMQSPTMLLFGLMGPLLLLGSWWSDRRNGKRVTR
                     AEQRRYAAALATAQALLAAALAAERDVRLAEDPDPAWLAGLARGPLTGLWDRT"
     gene            2720..3949
                     /locus_tag="PLB86_00015"
     CDS             2720..3949
                     /locus_tag="PLB86_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRC63552.1"
                     /translation="MADAVRQVEGEVRELIRRSGLDPARHPMEIDRLVRDAVTDYDER
                     SMRSGMPPLADLAGAAKSILDTVAGYGPLQRYFDDPKVEEIWVNSPDRVFIARAGQSE
                     LTNTILTADEVRDLVERMLRSSGRRVDLSSPFVDATLPDGSRLHVVIPDITRDHWHIN
                     VRKFVVRADHLDDLVRLGTLTRPAATFLEAAVVSGLNILVAGGTQAGKTTLLGCLASA
                     IPARERVVTCEEVFELKIPLRDVAGMQCRQPSLEGTGAVPLRRLVTEALRMRPSRIIV
                     GEVRQAESLDLLIALNSGLPGMCTLHANSAREAITKMCTLPLLAGENVSPRFVVPTVA
                     SSVDLVVHIALEGDGTRRVREIVAVPGRVEGDVVEVADIFTTRGGVLKRADGFPPHVD
                     RFERAGYRLTALLAQEA"
     gene            3949..4815
                     /locus_tag="PLB86_00020"
     CDS             3949..4815
                     /locus_tag="PLB86_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRC63553.1"
                     /translation="MSALAIGLLLGAGVFCIFWSTWPQTERSAPARRASLADRVRDQL
                     VQAGFDTISPGQLLAACMVVFVIVFLGAYSMTRVVPIAICFAAMAGYGPRALVTMRAR
                     KRRGQLRDLWPDAVDNIASAVRAGLALPEALSQLAVRGPEELRPAFAAFAEDYRTTGR
                     FHDCLNRLKDRLADPVADRLVESLRIAREVGGSDLGRVLRTLSAFLREDARTRAELET
                     RQGWTVNAARLAAGAPWVVLAMMSIQQDSLRAYSSAAGTAVLAVGAAITVVAYRLMVR
                     IGRLPEDERVLR"
     gene            4812..5753
                     /locus_tag="PLB86_00025"
     CDS             4812..5753
                     /locus_tag="PLB86_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRC63554.1"
                     /translation="MSGVGGWSMAGAAAGALFSVGVILIVRGVPVNRRPGLDARLAPY
                     LRDTIAPSRLLASMPRNQTRLSTLLAPLMGDLARRVDRIMGGSASVRRRLQRAGLPPD
                     VEGFRGEQVVWGALGGAVGSALGLLAILARGASVMPVVLLVVASVLLGVIGRDQWLSR
                     QARLREQLMLAEFPTVAELLALAVSAGEGATGALDRVCRLSRGELSIELGRCLADARA
                     GANLPTALQGLADRTGLVSLTRFVDGMVIAAERGTPLAEVLRAQAQDVREAGRRAVME
                     AGGRKEIAMMIPVVFLVLPVTVLFAVFPGFAFLRLTM"
     gene            5854..6084
                     /locus_tag="PLB86_00030"
     CDS             5854..6084
                     /locus_tag="PLB86_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63555.1"
                     /translation="MTPVHTAYLRTASGLGALWSAANRAARRARRDRGDVPGWVLITV
                     MTAGLVSGLWIVAGPALVDLFQSSLSSVTGKK"
     gene            6089..6499
                     /locus_tag="PLB86_00035"
     CDS             6089..6499
                     /locus_tag="PLB86_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRC63556.1"
                     /translation="MAAVAATRPGERGSAVAEFVMVSTLVLVLGMGVFQLGLVLHVRN
                     TLIACAAEGARLGARADVPTSDASERTGALVTDTLSAAYAQDISTRRTVTDAGVRVVE
                     VTVVAPLPVIGLIGPGGSMTVVGRAFDERQVIEP"
     gene            6496..>6929
                     /locus_tag="PLB86_00040"
     CDS             6496..>6929
                     /locus_tag="PLB86_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRC63557.1"
                     /translation="MTRLTGLVRWVRLRVGRPDEGRAIVEFVFLGVLLLVPLIYLVMT
                     LAVLQAAAYSATTAAREAGRAFTTAPAESQGMPRARAAAQVAFEDFGFLPEETSVAIT
                     CDGSPCLRPDARVSVTTSIRVRLPYLPAFLGDAVPAAIPVSAT"
     assembly_gap    6930..7029
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7030..7449
                     /locus_tag="PLB86_00045"
     CDS             <7030..7449
                     /locus_tag="PLB86_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HRC63558.1"
                     /translation="LILGMVMLAMLLIVGGVDVTAAQLARVRLVDAADAAALDAADAL
                     DEATAYRSGLSDAVVLSSATVKEAAAAYLSVRPKPEGVRAWGIAPGTGAQGGDTAVVV
                     LDATVELPMTGGLLAALGKEIAVHVEAKARAPLLPVP"
     gene            complement(7571..>8191)
                     /locus_tag="PLB86_00050"
     CDS             complement(7571..>8191)
                     /locus_tag="PLB86_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63559.1"
                     /translation="VASRFMGRIASEVIQANQAVASKVPGLGPLVSFGTSTASRLRGA
                     ADRQFEGLIGDAVGKGGTFAVRRLNRIFVETLRDPTTREAILQVWDQTSGERVIGLGA
                     YAAHAEVSGVVDAAHELAVTALGTEHAARVATLVVDALFEWFGGYTLAELLDQLEWPR
                     DEVIAHLVPLASFVVDTLRASGDLERLLRAQLEPFYASAEVADLLR"
     assembly_gap    8192..8291
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8292..8647)
                     /locus_tag="PLB86_00055"
     CDS             complement(<8292..8647)
                     /locus_tag="PLB86_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63560.1"
                     /translation="MPPRASRADTLAQRLLDAQVAYHLDRFTGDRLESTVAGLVESLL
                     DSAGPHQLADLIDREAVAAVVVRAMANVPGSAAVSGIVELGTQVAHDGPWQPFPVGET
                     VDREQVEVLVTSVLELT"
     gene            8880..9992
                     /gene="prfB"
                     /locus_tag="PLB86_00060"
     CDS             8880..9992
                     /gene="prfB"
                     /locus_tag="PLB86_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRC63561.1"
                     /translation="MAVDFGEEIKHLRATMDSVREVTNLDALTTEIARLEAEASVPNL
                     WDDPDRAQQVTSALSRANSERERVTGMDSRVEDLEVLVEMGTEEGDADTLAEAERDLA
                     KLKKDVGELEVRTLLNGEWDAREAVVTIRSGAGGVDAADFAEMLMRMYLRWAERHNYP
                     TTVLDTSYAEEAGLKSATFEVKVPYAFGNLSVEAGTHRLVRISPFDNQGRRQTSFAAV
                     EVVPLIEQTDHIDIPENELKIDVFRSSGPGGQSVNTTDSAVRMTHIPTGIVVSMQNEK
                     SQIQNRAAALRVLQSRLLLQKKAEEAATRKEMAGDVKASWGDQMRSYVLQPYQMVKDL
                     RTDHEVGNPSAVFDGDIDDFIEAGIRWKRNLEKGNG"
     gene            complement(10044..11297)
                     /locus_tag="PLB86_00065"
     CDS             complement(10044..11297)
                     /locus_tag="PLB86_00065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63562.1"
                     /translation="MAAAGEGEALTRRGIPLDPQLRVLAIGAFATRFGSGAVMTTSAI
                     YFTRHLGFTVGEVALAVSVAAAAAMIAALPAGHLGDTRGPREVLNVIMLALAVATPLP
                     VLATSPWVLTLLLAVEAALASAANAVRNGLTAQVAIGGRGVQFKAYLRAVTNSGIALG
                     SVVGGAALVVDTRAAYVSVFVMAGVLCGFAAWNTTRLTHLPPYRRPDGEPRLGVLRDL
                     PFVAVVLSTSMFSVHFVAAELGLALFVSQRTQAPQVMVAVLLLVNTVAVASLQVRLST
                     SADSVATGGRALVRGGALIAVGFALIGLADGSSAWVAVAVLLAGASVHVVGEMIGSGG
                     QWALQMGLAPHERQGQYQGFASLGFSLMNVVGPPVVTLLCIQGGRLGWVAMGLIVLVS
                     GVAAQALGRWALANRTAYGVTTHSG"
     gene            11551..12327
                     /gene="ftsE"
                     /locus_tag="PLB86_00070"
     CDS             11551..12327
                     /gene="ftsE"
                     /locus_tag="PLB86_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRC63563.1"
                     /translation="MILFDAVTKTYPKATRPALSEVSLEVERGEFVFIVGSSGSGKST
                     LLRLAIREERATSGAVLVAGRELRTLPDRRVPMLRREIGTVFQDFRLLPNKSVFQNVA
                     FALQVIGKSRALIRQTVPETLRVVGLEGKEKRLPHQLSGGEQQRVAIARAIVNKPTIL
                     LADEPTGNLDPATSRAIVELLDRINRSGTTVVVATHDHEMVNEFRRRVIELDAGKLVR
                     DEADGDYGKTTSAIPVVRIDEGDSDAEDGTPDAGSAGGER"
     gene            12327..13235
                     /gene="ftsX"
                     /locus_tag="PLB86_00075"
     CDS             12327..13235
                     /gene="ftsX"
                     /locus_tag="PLB86_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRC63564.1"
                     /translation="MRAGFVLGELGTGLRRNLSMAVSVILVTMISMYLLGLGLLAQRQ
                     VDTMKDYWYDRVQVSIFLCVEGSQYAACSGKATTPTQREQIKAQLEQMKPPVKEIFYE
                     SQADAFKRFKEQFKNSPLAEQATERAFADSFRVSLTDAGKYDVIVSSFSGAPGVGRVQ
                     DQKQLLDRFFAFMNAVSWGAIALSALMVLCAVLLMATTIRQTAFSRRRETGIMRLVGA
                     SNATIQLPFVLETLVSALVGASLAMGLLWATVSYGIGGYLSKALMDTAFIGMTDVFAV
                     APWVVGGVAIVAVLTSWFTLRRYLRV"
     gene            13352..14695
                     /locus_tag="PLB86_00080"
     CDS             13352..14695
                     /locus_tag="PLB86_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRC63565.1"
                     /translation="MLTSPPGRRPGLRAGAAGLVVLSFSAVLLAPPSAADTGDEKRAA
                     DAQVADLEEQLSETSAELAQAYTALTQTQAQLPAARARLASAQSAARAAAKHNAEVAA
                     ALAVAQANAARAEEAVARNTATRDQTQRTLDAFAADLFQGGSVGQLSVALGATSADDF
                     ATRLVLADTVTAMTNQALNDLAAAKADGDANGAYLAAVQVEVTDLKRQAENALAAAEA
                     SRNEAQAASSALDALVAQQSGYAASVEAQRANEASQLAEAQAEQQRLQALLIEQARIA
                     REREAARKAAEAAAQKAAEEAARKNGTAVPPPPVAQPDQASGFLSRPITGGRTSSEFG
                     MRFHPIFQVWRLHTGLDFANGCSSPVYAAAPGTIIRAGWAGGRGNQIVIDHGIRRGVD
                     LATTYNHLTSFVQRSGTVSRGQLIGYTGTTGNSTGCHLHFETLEDGQFVNPRSWL"
     gene            14777..15247
                     /gene="smpB"
                     /locus_tag="PLB86_00085"
     CDS             14777..15247
                     /gene="smpB"
                     /locus_tag="PLB86_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRC63566.1"
                     /translation="MVKESGRKVVASNRKARHDYHIEDTFEAGISLMGTEVKALRAGR
                     CSLVDGFAQFTGDELWLEGVHIPEYSQGTWTNHAPRRRRKLLLHREELTKIESKIREK
                     GRTIVPLAVYFKDGRAKVEIALATGKRQYDKRQALRERQDKREADRAMHSRSER"
     gene            15244..17295
                     /locus_tag="PLB86_00090"
     CDS             15244..17295
                     /locus_tag="PLB86_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRC63567.1"
                     /translation="MSHPARSHRHRRGYAARLALLVGAALTLLGVAAAPAALAQTPAQ
                     SSTTAAVAPWSDLSAAPALAALATADRMPAFDSAFVVDKDGGITVTETIQYSFDSSGG
                     ARHGIYRAITVRQGVDGRTDVYRYLSMTGVSVSSPTGADATVQLVDNGPDVTIRIGSA
                     SKTVSGTQTYVVRYHLANVLNPFTDTQTAEFYYNVIKDDTVAKGAVTVSVTAPVAATD
                     VRCTRGVGAACDSATGGATARFAVGSLAAGENLTIDTRYPLSGFSKIAPDIRTGTAGA
                     PGSGLDEGVAKVASLASVAGGVLAPIVALVVMGMLVATRGRDEWYAGLTPGLTPGLGQ
                     GPIADGIPPGGSAPTVRGKTPTIAVQFNPPPGVQPGLVGTVIDESADTLDVSATVIDL
                     AVRGYLRIEEVEGGGMFARTDWRLTALSPTTAHELRPYEQSVLVGLFQQGSPVLLSDL
                     KNHFAKTLAATKDLMYDEVLSRGWFRKSPQSQRTAWRGFGFFVMGAGIVTIFAYGSIS
                     RAIDISSGLPLPIPSGWVLGAGLLLAGFVIQLLGARMAAKTAAGSAVLAQSLGFRQYL
                     VTAEANQIRWEEAQDVFSRYLPFAIVFGVADRWAKTFQEVAAAAAAAGQVVIMPDWYI
                     YHGTLFPDFGSIVDGVDSFSTTASGTFQSTPGSSGGSGFGSSGGSFSGGGVGGSSSGS
                     W"
     gene            complement(17325..20753)
                     /locus_tag="PLB86_00095"
     CDS             complement(17325..20753)
                     /locus_tag="PLB86_00095"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HRC63568.1"
                     /translation="MFGKVLVANRGEIAVRAFRAATELGAKTVAVFPHEDRSSEHRLK
                     ADEAYEIGEPGHPVRAYLNPQAIVEVAVACGAQAVYPGYGFLSENPDLAEACESSGVV
                     FVGPPAAVLHLTGNKSRAIEAARRAGLPTLRGCPPGTDVEALVAGAEEIGFPVFVKAV
                     AGGGGRGMRRVDAPADLRGALAAAMREAESAFGDPTVFIEEAVIRPRHIEVQILADAV
                     GNVVHLFERDCSVQRRHQKVVEVAPAPNLDPALRDQMCADAVAFAREIGYVNAGTVEF
                     LLAENGRYVFIEMNPRIQVEHTVTEEVTDIDLVASQLRIASGETLPDLGISQDTITLR
                     GAALQCRITTEDPANEFRPDTGEITAYRSAGGSGVRLDGGTVFVGAQVSPHFDSMLVK
                     LTCRGRDFATAVTRARRALAEFRIRGVSTNIPFLQGVLEEPDFVAGRATTSFIDERPH
                     LRTARTPHDRGTKLLTYLADVTVNQPHGPAATRISPRAKLPTLDRYAAPPAGARDRLL
                     ELGPERFAAALRAQVPVAVTDTTFRDAHQSLLATRVRTRDLLHVAPHVAALTPQLLSV
                     EAWGGATYDVALRFLAEDPWDRLAALRAAMPNLPLQMLLRGRNTVGYTPYPTTVTDAF
                     VAEAAATGIDIFRIFDSLNDVSQMRPAIDAVRATGTAVAEVALCYTSDLNDPGERLYT
                     LDYYLHLAEAIVAAGAHIIAIKDMAGLLRAPAARRLVTALRERFDLPVHLHTHDTAGG
                     QLATLLAAIDAGVDAVDVASSSMAGTTSQPPMSALVAATNATERATGLDLAAVCSLEP
                     YWEAMRTLYRPFESGLPSPTGRVYTHEIPGGQLSNLRQQAIALGLGDRFEAIEDMYAA
                     ANKILGNIVKVTPSSKVVGDLALALVGAGADPAAFEADPTAYDIPDSVIGFLGGELGD
                     PPGGWPEPFRTKALAGRTIKRAETELAPDDLAALAVSPRATLNRLLFPGPTKEFLASR
                     EAYSDLSIVGTVEFLHGLVPGEEHSIDIEPGKRIIVSLQAVSEPDERGFRTVMCTLNG
                     QLRPVQIRDTSVKVDVRQAERADPAIKGHIAAPFAGMVTPAVREGDTVAAGSVVATIE
                     AMKMEASITTPVGGVVERLAIGGHTQVDGGDLILVVQVGQPPAPKAPEIEASAG"
     gene            20844..22097
                     /locus_tag="PLB86_00100"
     CDS             20844..22097
                     /locus_tag="PLB86_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63569.1"
                     /translation="MTSTLPTLAAVQRRTIATLLSTQVLGGLGVGAAISVNAMLAKDV
                     SGVESLAGLAQTAAVLGTALVTLLIARVMDDHGRRIGLTLGYALGFIGTALSIVAGVT
                     RVFPLLLVGAVFLGATTAANNQSRYAATDLSLPAHRGRDLSLVVWATTVGSVLGPNLS
                     GPGKVVASAVGLPPLTGAYLFSGVGMLAAMAVMFFRLRPDPLVLARAEHLKELPAGTA
                     PTARASGRALLRENRLLRLAAIGMSLSHAVMVSVMVMTPIHMDHGHASLEIIGLVISI
                     HILGMFVFSPAVGMAVDRWGSAPILAAGGVVLLVALVLAGSSHAGASWTLGLGLFLLG
                     VGWSLGTIACSTLVTAASTPHTRPAIQGVTDLLTSLAGALGGAIAGLVVGLAGYSALN
                     IGAAVLAVGVVTTAVAVGRQTESAD"
     gene            22140..23672
                     /gene="mptB"
                     /locus_tag="PLB86_00105"
     CDS             22140..23672
                     /gene="mptB"
                     /locus_tag="PLB86_00105"
                     /inference="COORDINATES: protein motif:HMM:NF038066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HRC63570.1"
                     /translation="MPSSPGHDRAHVAPAGWTGRIRREFDDLRYAWGDPVTRWGALGS
                     LLILIGAYGPSTMPNSNFWRDLPVIGLMQTTLPGRILATGSMLIGVVILLDAWLRLRP
                     SKGHRPLTRGTVWWWSLPLLLSPPLLSFDAFSYAAQGELVSRGFDPYKVGPGVLGDAF
                     TSQVDQMWRDTPAPYGPLSLQLQHLIVVVTGHQPYLSALLVRIPAFIGVLLLAVYLPR
                     LARRIGYDRETTIWLAVLNPLVLLHLVGGAHNDALMIGLMMWGLWVAAKTHWVPAAVI
                     IAAAASVKQPAAAAVLAVAALHAWRVGGWREQPSNATVARYAVGVVGVFLAAFVAIGL
                     LTGLGFGWITALGVPNAVRSFLSPVTTIAQGLDWVLVSLGVIPDADLVVPIAQRVGQV
                     VGLLVVIWMVFKFKARHPVRTLGWILLVLVFTGPVVHPWYILWGGLLFAMTESGQLAR
                     RIVVWATCGLAMYSVVDAAWRSSVVAVGVTAALALVWITTGHDRDLIRDKDFAKSRRT
                     PA"
     gene            23778..24144
                     /gene="ssrA"
                     /locus_tag="PLB86_00110"
     tmRNA           23778..24144
                     /gene="ssrA"
                     /locus_tag="PLB86_00110"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(24281..24709)
                     /locus_tag="PLB86_00115"
     CDS             complement(24281..24709)
                     /locus_tag="PLB86_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HRC63571.1"
                     /translation="MRTILNIIWVVFAGFWLWLTYLAAGVLACLLIVTIPWGIASFRM
                     ANYALWPFGRTIVDKPDAGPASTIGNIVWIVCFGWWLALGHLTTAIAQAVTIIGIPLA
                     VANVKLIPVSLVPMGKDIVRIDDLGKATPWGPGPQRSFSV"
     gene            complement(24764..25198)
                     /locus_tag="PLB86_00120"
     CDS             complement(24764..25198)
                     /locus_tag="PLB86_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRC63572.1"
                     /translation="MATTELTADTFERTILDNDIVFVDFWASWCGPCRQFGPVFEKTS
                     AEHPDAVFGKVDTEAEQALAGALEITSIPTIMAFREGILVFSQPGALSAPVFEDLVGQ
                     VKALDMTDVRRQIAEHEAGHETGHEAGHDGAAEPQGAGTSQA"
     gene            complement(25267..25935)
                     /locus_tag="PLB86_00125"
     CDS             complement(25267..25935)
                     /locus_tag="PLB86_00125"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRC63573.1"
                     /translation="MSMPSEHRGPRPARVEVTGTGTASATPDVVRVALGIRCDSEGVA
                     AALSAAADTVRQVSAAARAHGLADRDIASTSASVQPRWNNDGSAVAGYTAYHQLTLLV
                     RSLPSLNDLVDAVADAAGNALVIDGITLDLAERGPLAEHAREAAFDDAQDKAQQYAAL
                     AGARLGRVLAVVEGGGVPGFEPMAGRRDAMMSMKASAGGMPVEAGEHSVGATVRVAWE
                     LVPE"
     gene            26115..28562
                     /locus_tag="PLB86_00130"
     CDS             26115..28562
                     /locus_tag="PLB86_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC63574.1"
                     /translation="MPTPKRSGAPKKARWTTAQKTASKSGPKKPHRGQAPTESKPPRA
                     ERAPRHVARTGSGDAPTSRYAARSRDERPARFERDERPSRPARFVRDDRPERPTRFDR
                     TDRPARFERDDRPARFERDDRSARPARVDRDGNPTRPARFERDDRPRRFDRDDRPARF
                     ERDDRPRRFDRDDRPARFERDDRPRRFDRDDRPARPARDDRPARFERDDRPSRPARDD
                     RPARDDRAARFERDDRPRRFDRDDRPARDSRPARDDRPARDDRAPRFERDNRLARPGR
                     FDRDDRPARPGRFDRDGGPSRPTRFERDDRPRRFDRDDRPARRDGERAAYREPFVDPE
                     SERLEADSWTSSARTPVSTNPVTVAEDNGFASLGLPERLVERLARDGITTPFPIQDAT
                     IPDALAGRDVLGRGQTGSGKTLAFGLPMLARLLADGSGRVPRRPRALVLVPTRELAMQ
                     VSDALEPLVHVVGMRHKLVAGGLSYTTQISALERGIDLLIATPGRLIDLMDRGAVELG
                     SVQVAVLDEADHMAEMGFLPEVTRILDEVPDGGQRLLFSATLDNGVDQLVSRYLTDAV
                     THSTDDATASVTTMRHFPFLIDPQHKKPLTAELANREGRTIVFVRTKLGADRVAAQLR
                     ECGVVAAALHGGLSQGVRNRVLGAFRLGTLRVLVATDVAARGIHVDDVSLVLQVDPPA
                     DHKDYLHRSGRTARAGEEGSVVTLVLPHQRKQTERLLREAGLEEVPTRVSPGDSLVAA
                     AGGSAPTGEPVTDEAMRRLLEPERRSPAGARGPRHGSHGGFRPHHGGAPRREDRRFDT
                     RDDRRPTSRDARRSVRD"
     gene            28567..29157
                     /locus_tag="PLB86_00135"
     CDS             28567..29157
                     /locus_tag="PLB86_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRC63575.1"
                     /translation="MGSESTEPSGQPTAYLAGKLLVATPSVEGDVFRRSVVLMLHHDA
                     DGAQGVVLNQPLEAGVDAVLPGWQAVTTAPQTLFRGGPVGTDSAIGIVSVPGEGEATL
                     GVQRLFGGIGVVDLDAPPLLVAPEVAGLRIFAGYAGWSEGQLEGEIARGDWYVLDAEP
                     RDAFTDDPETLWASVLRRQRDSLALVAFYPDDPSMN"
     gene            29252..29665
                     /locus_tag="PLB86_00140"
     CDS             29252..29665
                     /locus_tag="PLB86_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRC63576.1"
                     /translation="MTYAGDISPTDTFAALQADPQAVLVDVRTSAEWAYVGVPDLARV
                     GRDAILIEWVTYPGGARNDNFVDQLREAGIAEGQSVYFLCRSGVRSKHAADAATEAGL
                     GPAYNIVDGFEGQLDATHHRGVGGWKTAGLPWRQG"
     gene            29662..30858
                     /locus_tag="PLB86_00145"
     CDS             29662..30858
                     /locus_tag="PLB86_00145"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HRC63577.1"
                     /translation="MSVGDEVRRWRPDTYAVRGGLTRSEFDETSEAVFLTSGFVYDSA
                     QQAEAAFKDEIDKFIYSRYGNPTVAMFEERLRLIEGAEACFGTSSGMSAVFVALAALC
                     GAGDRIVASRALFGSCFVIIDEILPRWGVERVLVDGADLDQWRAALDRPTAAVFFETP
                     SNPMQELVDIRAVSDLAHAVGAKVVVDNVFGTPVYSKPLEHGADIVVYSATKHIDGQG
                     RALGGAILGPADYIGGPVKNLMRHTGPALSPFNAWVMLKGLETMSLRVNRQHDNALAV
                     AAFLAEHPRVTRVLHPWREDHPQHDLARRQMLGGGTVITFAIDGGKDDAFAVMNALQV
                     VDISNNLGDAKSLVTHPATTTHRRLTPEARAAVGITDGTIRLSVGLEDPADLIDDLAA
                     ALDALG"
     gene            complement(30871..31419)
                     /locus_tag="PLB86_00150"
     CDS             complement(30871..31419)
                     /locus_tag="PLB86_00150"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRC63578.1"
                     /translation="MGLMTVLARTGEFTREDLDAMPDDGHRHELLDGSIVMTPSPGYR
                     HQRMSLRLAVLLLGACPEGLEVLTAPFDVVLSPRTVVVPDLIVARSGDFTEKDLPRAP
                     LLAVEILSPSTRRLDLTLKRDLYAEAGTAAYWVLDPQVPSITTWTLRGGTFEQTGYAA
                     AADSLTADLPFPVTVCPGDLVR"
     gene            31526..31852
                     /locus_tag="PLB86_00155"
     CDS             31526..31852
                     /locus_tag="PLB86_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HRC63579.1"
                     /translation="MPRASSMPPDPFEPQPMPSEPGTGTRTSVLEREETVVEPQVQEP
                     GDHERFSHYAHKDKIMEAMVMGTPCVALCGKVWIPSRDPKKFPVCPTCKAIFEDDSAG
                     WVPEDE"
     gene            31917..32768
                     /locus_tag="PLB86_00160"
     CDS             31917..32768
                     /locus_tag="PLB86_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRC63580.1"
                     /translation="MSIIEGTKQAATDRLRVIAGPAYRRVKREIQWRRCGADLALERG
                     PVSGFPWVVMAHETPLIRPLSGLEHRLQVNKVTNLRLAIEPLDGRVLQPGQRLSVWWF
                     VRAPTARRGFLEGLVLSQGRLTAGVGGGLCQLTNLIHWMTIHTPLTVVERWRHTYDVF
                     PDAGRTQPFASGATCSWPSLDLQIENRTDTAYRLGLRLTDTHLVGEWRAAVEPTRSYE
                     VYEAAHEIATELPGRFVRRNLLRRKVFDMVGEQIDDEFVAENHALMMYPPFLSDRPTT
                     PPGPSPE"
     gene            complement(32781..34163)
                     /locus_tag="PLB86_00165"
     CDS             complement(32781..34163)
                     /locus_tag="PLB86_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRC63581.1"
                     /translation="MTEAPFRTVFEPFRIHSVEPLRMTTADYRRGKVREAAYNLFQLH
                     ADDVLIDLLTDSGTGAMSRDQWAAIQHGDESYAGSPSYFIFRDAVQELFPYAHVIPTH
                     QGRAAERILFQALAGPGKVIPSNTHFDTTRANIEYTGAEAVDLVIAEGRDPAALHPFK
                     GNIDLDALETILRERGSDIPCVMMTITNNSGGGQPVSLANLRAAAEIAHRHGKPLFLD
                     AARFAENAWFIHEREPGQGERDVRDIIRDIAACADGMTMSAKKDPMGNIGGWLALNDD
                     EIAGKCRNLLILTEGFPTYGGLAGRDLEALAQGLKEVVSHDFLRYRIRSTAYVADALE
                     ALGMPCIKPAGGHAVYIDAKALLPHIPPLQFPGQALAVALYEHGGIRTCEIGSAMFGR
                     HPDGSESPAAMELVRLAIPRRTYTQSHMDYVIEVAERVMAVKDSLPGYQIVVEPPQLR
                     HFTAEFAPLG"
     gene            complement(34236..35555)
                     /locus_tag="PLB86_00170"
     CDS             complement(34236..35555)
                     /locus_tag="PLB86_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC63582.1"
                     /translation="MTDHAEKSTYAPRPQTRIAHPQWARAATIYQLNQRQFTHEGTFQ
                     AAREHLPRLRRLGVDIIWLMPVNPIGEERRKGGLGSPYAVRDYRAVNPEFGSLRDLRS
                     FVAAAHALGMKVILDWVANHTSWDNVLVAEHPEWYARDWKGDFRPTPWWDWDDVIDLD
                     YSHAGLRRYMTESMCYWVREADVDGFRCDVAGFVPLDFWEQARAELDAIKPVFMLAEW
                     EARDLHEAAFDMTYGWSWNETMHHIAVGRSDVTALHIFYAWNESSWPRDAIRMMFVSN
                     HDKNSWEGTEFEQFGPMRESAIVLSVIGEGMPLIYNGQEAGFDKRLAFFDKDEIIWRE
                     DPQGELYRTLFALKKAHPALWNGAAGGRMVKVPNDMETSVLTFVRTQGEDSVFAVFNF
                     TGVHRSVTLGRGPQDGPWVDAIDGSPLRGREGMRIDLAAWGWRVFVR"
     gene            complement(35592..36287)
                     /locus_tag="PLB86_00175"
     CDS             complement(35592..36287)
                     /locus_tag="PLB86_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRC63583.1"
                     /translation="MPTSHSPEAFQAHLERVAGAPLPDFHHFQRAMSWAVIEPRELLF
                     DAGTTDRRFFFIVDGIVSLATSDRPGRDWIRRFVGPGELLSSVPALAHPLPRAAAAFV
                     GLDPDSADFGPSAYMHYSARGITRVRARYVDISTFWALSNRYREWQQLALIASIASTA
                     LQERRERELLTLNAEERYRLLVQERPELVSLVPQKDLARYIGVTPVAMSRIVSRVRQG
                     LVAEGPSRAAVSG"
     gene            36524..37405
                     /locus_tag="PLB86_00180"
     CDS             36524..37405
                     /locus_tag="PLB86_00180"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRC63584.1"
                     /translation="MTQPSGWYDDPSDHSRLRYWDGILWTDRTVPKQSPTAADSTIGR
                     AVDPYAASSHHAPTGPGPGPYSPAAPTYGQPPTPYGQAPTPYGQPPAGRDYGAAPTYQ
                     WRPSGPTTPDGVPLAQWWQRLVAAVIDNIIVQLASLVLAFPWWRDFLVWYLDFLQTAT
                     VSSGSVDPLTLFGEINTEMARYIVPIVLIQSAVTFVYHVLFLHRNGASVGKLALGIRV
                     RRFDRPGPLTLLEASRRQALQLVFGLVGLVPFIGMVTGVVGYVDVAWLLWDQRRQCLH
                     DKIADTLVEQKPPTRRV"
     gene            complement(37427..37957)
                     /locus_tag="PLB86_00185"
     CDS             complement(37427..37957)
                     /locus_tag="PLB86_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRC63585.1"
                     /translation="MPNNPRALPPGPAPRTPAPVDDVDRAIVDALVADARVPNVQLAR
                     AVGLAESTCIGRVRSLLARGILRGFTARVDRGKLGYPIEAMVAVRLAGTDRRRVDEFG
                     EHVSRLPGVLAAYNVSGADDFLVHLVAQSPDTLRGVVLDHLSGHPGVVHVQTSLIFRT
                     HEGRHSLPAPGPVATN"
     gene            38067..39278
                     /locus_tag="PLB86_00190"
     CDS             38067..39278
                     /locus_tag="PLB86_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRC63586.1"
                     /translation="MRADTSAVHAGREDLVALGVHVPPIDLSSTYPIGDLDAGGAAYE
                     TLAMGGRGEAAATLVYQRLWNPNVDRFERALAQLEGTEEAVAFASGMAALTATLIACV
                     AAGTPHIVAVRPLYGGSDHVLATGLLGTQVTWATAATVGEAITDRTGLVLLETPANPT
                     LDLVDIAAVVAQAGRVPVMVDNTFATPVLQQPRRQRATLVLHSATKFIGGHGDLLGGV
                     IAASPEWAVRLRQVRALTGALLYPLAAYDLHRGLQTLPVRVRAQQDNAIALAGWLAGH
                     PAVERVHYPGMPGADPEGLLGRQLAGPGAVLAIALRGGFDAAADLVTRTRLVTHAVSL
                     GGVDSLLQHPASLTHRPVAAEAKPHPNVVRISVGLEDVRDVIADFDQALRGVGERRLV
                     DVLSGADALAR"
     gene            complement(39250..40491)
                     /gene="fahA"
                     /locus_tag="PLB86_00195"
     CDS             complement(39250..40491)
                     /gene="fahA"
                     /locus_tag="PLB86_00195"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HRC63587.1"
                     /translation="MTWLDLPADHPFGLAHLPYGVFSTVADPAPRVGVRIGDLVLDAA
                     RAAAATGYAAGAAWATPSLNAFLGQGTTAWAAGRAWLTQVLNDAAYQHAVQPHLLPLD
                     AVTLHLPITVGDYVDYYASAHHARNVGMIFRPDSPALPVNWKHLPIGYHGRSGTVVVS
                     GTPFHRPCGQRKGPQDPVPVYGPSVRLDIEAELGYVVGGATSMGERISVDEAGEHLFG
                     VVLLNDWSARDIQAWEYVPLGPYLGKSFCTSISAWVTPMAALQSARVDLPRDEADPEV
                     LPYLRGTGTGVQGSGGAAFGLDVHLEVRLNGEVVSRPEYRDMYWSPAQMLAHMTVNGA
                     SVGDGDLFGSGTISGPEKGQRGSFLELSWGGQEPFELADGSSRSYLQDGDEVAITAWA
                     PGPDGTRIDLGDVSGRVRPPR"
     gene            complement(40488..41330)
                     /locus_tag="PLB86_00200"
     CDS             complement(40488..41330)
                     /locus_tag="PLB86_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63588.1"
                     /translation="MGSRLPRALIERLVDDAAVFPPGLAPLPRAVAEHLARTAYREFV
                     GPLLVPATAAAEVRALAGGARLRVGLIARPGMPVEPVRAAAAALAGSTVEVAGVEVGA
                     GAEWESLLDLGVALTVEIPRDGFTAAIDAVAAVALRTGREPLVQAKFRTGATEVWAWP
                     DEVELARFFAACRERDLPFKLTGGLHHVLRADHPGPDGGPQHGLLNVLAAVHATAEGV
                     PEPEVAELLGERDAAPLVDLLTALGTDAASRVRCAFTAYGCCTVADPLGELAALRLID
                     KEHQ"
     gene            41454..42113
                     /locus_tag="PLB86_00205"
     CDS             41454..42113
                     /locus_tag="PLB86_00205"
                     /inference="COORDINATES: protein motif:HMM:NF018745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HRC63589.1"
                     /translation="MKNSTGMFGPDSPRGARLGWSGVIGAVTFAVASARLGPAVAAVL
                     GWILGAGAFVLWTLVVVGRLDGPGAESHATREDSTRVVSDLILIGGSIASLGGVGVLL
                     ATHRGEGGAPWQAVLGVLCVAVSWVLVHTVYLLRYAALYYAQTPRGIDFNQTEPPDYH
                     DFAYLAFTLGMTYQVSDTAISSSVFRRTVLRHTLLSYVFGSVILATTINLVVQLASPT
                     G"
     gene            42183..42473
                     /gene="clpS"
                     /locus_tag="PLB86_00210"
     CDS             42183..42473
                     /gene="clpS"
                     /locus_tag="PLB86_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRC63590.1"
                     /translation="MSTAPVKEPEVISLEASELALPWITLVWNDPVNLMSYVVYVFET
                     YFGYPRAKAERLMMDVHTLGKAVVSLGTREAMETDCMAMQGFGLWATFEKNQ"
     gene            42473..43087
                     /locus_tag="PLB86_00215"
     CDS             42473..43087
                     /locus_tag="PLB86_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HRC63591.1"
                     /translation="MARAFRRRGARMVARLDRNEREVIASLMEQTRSLLAPDVPSTGD
                     AFTDLMASLESGATAPEGLPRGTDPGDRDPALARLLPDAHRGDPAVAAEFRRLTEQDL
                     RRRKSDALTASIDALRAEADRDRLSLEPATAQSFLIALTDTRLLMGERLGLRTEEDVD
                     AWEARVEADPDDPLAYAAALYDFLTWLQDGLVGALMGRRAFPDA"
     gene            43223..44032
                     /gene="murI"
                     /locus_tag="PLB86_00220"
     CDS             43223..44032
                     /gene="murI"
                     /locus_tag="PLB86_00220"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRC63592.1"
                     /translation="MGDAPIGIFDSGYGGLTVARAVLDQLPHESIVYFGDTARTPYGP
                     RPIGQVREFALECLDHLVDRGVKALVIACNTASAAMLHDARERYDVPVIEVIRPAVRR
                     AAAATRTNRVGVISTMGTHASRAYPDTFAAAPHIEVTSQPCPRFVEFVENGVTGGDEL
                     LAVAREYLAPLQEAEVDTLILGCTHYPLLTGVISYVMGDLVTLVSSAEETAKDVYRVL
                     ADGGALRPDGLPAPRHEFVTTGDPQTFSRLARRFLGPEAAIESRREAVAPG"
     gene            44053..44814
                     /locus_tag="PLB86_00225"
     CDS             44053..44814
                     /locus_tag="PLB86_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC63593.1"
                     /translation="MRVTIVGCSGSFPGPQSPASCYLVQAEHEGRTWSMVLDLGSGAL
                     GPLQRYLDPTTLDAVVLSHLHADHFLDLCGLYVMQRYHPRGFRGPVPVWGPAGTPQRV
                     GLAYYGHDGTEVSEFSFTRVVDRATFRIGPFSVTARRVNHPVEAYGYRVECAGQVLVF
                     SGDTDMCDALIDLAADADLLLADTAFVDGRDDERNIHMTGSRVAQVALAAGGVKRIML
                     THLPSWNDPEVCRAQAAAVWPGQVEVAAPGLVIDL"
     gene            44891..45643
                     /gene="rph"
                     /locus_tag="PLB86_00230"
     CDS             44891..45643
                     /gene="rph"
                     /locus_tag="PLB86_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRC63594.1"
                     /translation="MSNATRHDGRAQDQTREVRITRNWLDHAEGSVLVEFGRTRVLCA
                     ASFTEGVPRWLKGQGKGWVTAEYAMLPRSTNTRSDRESVKGKIGGRTHEISRLVGRSL
                     RAVIDMKALGENTIVLDCDVLQADGGTRTAAITGAWVALADAVADAKARGLIKPDAAP
                     LTGSVAAVSVGVVGGIPVIDLDYPEDSTAETDMNVVMTGDGRFVEVQGTAEGEPFDRA
                     LLGQLLDLATKGCADLTAAQHAALAADPRSRA"
     gene            45663..46277
                     /gene="rdgB"
                     /locus_tag="PLB86_00235"
     CDS             45663..46277
                     /gene="rdgB"
                     /locus_tag="PLB86_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRC63595.1"
                     /translation="MTRRLVLATRNAHKVGELREILADVLADLDLQIVGLADFPEVAD
                     VVEDGVTFAENALLKARAVAAATGLPALADDSGLAVDVLGGAPGIFSARWSGRHGDDL
                     ANLELLLAQVSDVRDEHRGAGFVCAAAIALPDGRAHVEEGVFRGTLTREPRGSNGFGY
                     DPILLVEGDVRTSAELSPAEKNAMSHRGKAFRALAEHLGGMLEA"
     gene            46274..47911
                     /locus_tag="PLB86_00240"
     CDS             46274..47911
                     /locus_tag="PLB86_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003797900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRC63596.1"
                     /translation="MTSEQPGQSGPQGVLPVPAWDAPDPSEPPGDPFGPGGWAVGNLG
                     YAVDGLVAGATGLLVAEALDVAFTLGGWVSGNSSGRSPILAVGAAFVDLTPAWLKEWA
                     VASFGTGDKAVLFAGMAIVLAVLFVAIGLVARTRHTLATALVGVLGVLALVAQLTRAG
                     ASPTDPIPLVGGLAVTLWMLPRLVARTAIEGPSRREALGLAGAAAGVALATAVRGSGG
                     VIAANASASRAAAVPVPSGLPIPAAAGAEIGVGGVGPWAVPNADFYRIDTALTVPRLT
                     AEAWSLRVWGEVEREVTLTWAQLVGKPLIQRYVTLACVSNEVGGHLVGNALWTGWPVR
                     ELLREAGAKAGADMVLSRSIDGWTAGTPLSALTDDRDALLAVAMNGVALPFEHGFPVR
                     LVVPGLYGYVSATKWVTDLKVTRFADDMGYWTPRGWSALGPVKTASRIDVPRSGARLP
                     AGRVAVAGVAWAMHRGIQAVQVQVDGGPWADARLATEPTVDSWRQWVFEWDAGPGSHV
                     LTVRARDGAGEWQTAVQTTSDPDGATGHHSVTVAVAG"
     gene            47945..48026
                     /locus_tag="PLB86_00245"
     tRNA            47945..48026
                     /locus_tag="PLB86_00245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47979..47981,aa:Leu,seq:tag)
     assembly_gap    48038..48137
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<48138..48739)
                     /locus_tag="PLB86_00250"
     CDS             complement(<48138..48739)
                     /locus_tag="PLB86_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC63597.1"
                     /translation="MTTPVLDVQGLYYVYPDGTRALDGADLHVHRGERVALLGPNGAG
                     KTTLVLHLNGILGGDAGSGGGTVEVSGMPVGKDTLPEIRRRVGIVFQDPDDQLFMPTV
                     RDDVAFGPANLGLRGAELEARVAEALAAVGMAAYADRPPHHLSFGQRRRVAVATVLAM
                     RPEILVLDEPSSNLDPASRRELAEIILGLDVTVLMVTHDLP"
     gene            complement(48736..49500)
                     /gene="cbiQ"
                     /locus_tag="PLB86_00255"
     CDS             complement(48736..49500)
                     /gene="cbiQ"
                     /locus_tag="PLB86_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HRC63598.1"
                     /translation="MGAEHAHGLHLPGQSTVHRLPAHLKIVSTLAFVLLVVATPREAT
                     WPYAVHLAALMLVARTARVPAAYLATRLVVEVPFVVFAVVLPFVATGPRISVLGLSVS
                     EPGLVAAWALLAKGTLGVLAALLLAATTEPRDLLTGLTRLRLPRQLVEIMGFMVRYVE
                     VVTGDLRRMSIALASRGFDARSPRQWPVLATCLGALFVRSYERGERVHLAMLSRGYAG
                     SLPVLDAAPLRVGDLVVAAALPTMAAVGMGAAVLTR"
     gene            complement(49490..49762)
                     /locus_tag="PLB86_00260"
     CDS             complement(49490..49762)
                     /locus_tag="PLB86_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HRC63599.1"
                     /translation="MSGHGRRWWALGVLVTLLVAGVLSRFASTAPDGLERVAADQGIS
                     GQAVERSGLLSYDGLGGVVGVALVLIVGLGLTAVVRRRSARSPRGR"
     gene            complement(49759..50460)
                     /locus_tag="PLB86_00265"
     CDS             complement(49759..50460)
                     /locus_tag="PLB86_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRC63600.1"
                     /translation="MHVPDGFLDLPTSLATAAVAASAVALSLRGARRELDDRAAPLAG
                     LVATFVFAAQMINFPVAAGTSGHLIGGALAAVLVGPWTATLCLTVVLLVQGLLFADGG
                     LTALGTNITLMGVVGVWTGYAVFRLALAVLPRRIGSVPLAAGIGGFVSVPMAALTFAG
                     LFMVGGTAPLDAGSLVAAMASVHTLIGVGEGVLTALVVGSVVAVRPDLVHGARRIRAA
                     SIQAAAPAPSSGQRA"
     gene            50614..51081
                     /gene="bcp"
                     /locus_tag="PLB86_00270"
     CDS             50614..51081
                     /gene="bcp"
                     /locus_tag="PLB86_00270"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRC63601.1"
                     /translation="MTTRLQAGDIAPEFTLSDDTDQSVSLADFRGQRVIVYFYPAALT
                     PGCTTQACDFSDSLDALAAQGYAVLGISPDAPAKLAKFRAKEGLTITLLSDPDKSTLT
                     AYGAFGEKKLYGKTVVGVIRSTFVVDADGVIADARYNVKATGHVAKLRHDLGL"
     gene            51126..51392
                     /locus_tag="PLB86_00275"
     CDS             51126..51392
                     /locus_tag="PLB86_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HRC63602.1"
                     /translation="MSEQGQSVEQLQAEIEAARSRLSRTIDELTYRAQPQQIVARQTE
                     AAKASLMAATHDDDGGLRTERIAAVLAVVAALAIGLGLLRRRRG"
     gene            51459..51755
                     /locus_tag="PLB86_00280"
     CDS             51459..51755
                     /locus_tag="PLB86_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRC63603.1"
                     /translation="MLFDRVLVSIDSEKGERTSGGGILIPATASVGKRLAWAIVVATG
                     GNVRQVQPGDRVLYDPEDRHEVELQSKGYILLREKDIHAVAASRVDDGNTGLYL"
     gene            complement(51838..51912)
                     /locus_tag="PLB86_00285"
     tRNA            complement(51838..51912)
                     /locus_tag="PLB86_00285"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51878..51880),aa:Lys,seq:ctt)
     gene            complement(52010..52660)
                     /locus_tag="PLB86_00290"
     CDS             complement(52010..52660)
                     /locus_tag="PLB86_00290"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HRC63604.1"
                     /translation="MVVGRFVAVAVAGLAVTLGSLTSPPSAVATPMPYAAALAPAHSA
                     LVSAVPADQAVLASGPTELVLTFNEEINPRFAQLALSRSGDVVALNPATATGTVLRAT
                     LADPGPGTYRIAYRVVSADGHPISGETSFTVTGPTRTPTSTGPTPTVAPSAPNTTSAP
                     SNPSAGSGAATTDSMASATPTTGSQRQDGMTWLYIGGALLALAGLTGVWESRRKRR"
     gene            complement(52744..52819)
                     /locus_tag="PLB86_00295"
     tRNA            complement(52744..52819)
                     /locus_tag="PLB86_00295"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52784..52786),aa:His,seq:gtg)
     gene            complement(52853..53557)
                     /gene="orn"
                     /locus_tag="PLB86_00300"
     CDS             complement(52853..53557)
                     /gene="orn"
                     /locus_tag="PLB86_00300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HRC63605.1"
                     /translation="MSTNASTTPTSSPTAGSSGGDRALADRIVWIDCEMTGLSLQADA
                     LVEVAALVTDYELTVLGEGVDVVICPPPESVAQMDPFVVDMHTTSGLLTELPTGVSLR
                     EAEERVLAYVREWVPEPRKAVLAGNTVGTDRTFLARDMPELEGWLHYRVIDVSSIKEL
                     SRRWYPRAYHNSPSKSGGHRALADIRESIAELRYYREAVFVPQPGPDSATARALGERH
                     VVVHPAVNSTVADPVR"
     gene            53618..54814
                     /locus_tag="PLB86_00305"
     CDS             53618..54814
                     /locus_tag="PLB86_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HRC63606.1"
                     /translation="MSTGGSGVPGWDSGTAAYPMPGPGVPNPTRRPERRGWVTTGVII
                     SVFLISLAMLSVFFGIDIGIGNSLVAAAVSAVPLLVVVPVFLWVDRMESEPVRYLIFA
                     FLWGALCAAVGAIILNTGIHLIFLAAHTDNADAVTAVVSAPLVEETLKGAAILFIAWR
                     RRREFDGVIDGIVYAGMAGAGFAFSENILYLGRAFAEAGTSGLTEVFFLRCLMGPFAH
                     PLFTACTGIGIGLAATVARSAAARIAYGTAGLVAAMGLHAIWNLSASVGAYLQVYLAF
                     QMPIFAVFLVGLVLLRQREAGLVRRYLSQYADAGWFTRAEVEMLSSMGQRRSARAWAR
                     GLGGRAAVRSMRAFQDSASDLALLRARIVRGSAEPGAGAHERDLLDAVTANRGVVVGQ
                     VSPRDR"
     gene            54942..55014
                     /locus_tag="PLB86_00310"
     tRNA            54942..55014
                     /locus_tag="PLB86_00310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54975..54977,aa:Arg,seq:tct)
     assembly_gap    55054..55153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            55339..57018
                     /locus_tag="PLB86_00315"
     CDS             55339..57018
                     /locus_tag="PLB86_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HRC63607.1"
                     /translation="MLLAAVNGLRDQVEASRLPLALPDAPAAGKVRLELLQQLDDYVI
                     PRLSSLDAPLLAVVGGSTGAGKSTLVNSIVGMQVTRPGVLRPTTRSPVLVHHPSDEHW
                     FVDTRVLPGLARVRGGDLAEDPSAVRLVANDALPAGLALLDAPDIDSVVASNRDLAAQ
                     LLSAADLWLFVTTAARYADAVPWTLLRQASERGTSVAIILDRVPPEAVGEIRDHLTAM
                     LKEQGLALAPLFPIPEVPLGADGLLGDAEVGRLKGWLSALANDARARALVVRHTLSGA
                     LDSIDSRIAVLAEATGEQQAATNALARVADAAYAEAADGVRKGMLDGTLLRGEVLSRW
                     QEFVGTGEFFKQVESAVSRMRDRITAAVKGQPAPGQQLGEALQTGVAALVTAEGSAAA
                     SQVARSWRGMPGGQLLLDATPALAVPSADLTTQIETLVREWQDDVLDLVRNEGRDRRT
                     TAKVAAYGVNGLGVVLMLVTFAHTAGLTGAEAGIAGGTAVLGQKLLEAIFGDQAVRSL
                     AAKARAALLDRVEDLYAAESGRFHAAIDAVSVERDQGRKLSAAALNVRAAR"
     gene            57015..58736
                     /locus_tag="PLB86_00320"
     CDS             57015..58736
                     /locus_tag="PLB86_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HRC63608.1"
                     /translation="MSPLRMGSATVTVVTAAELADRAAALSQACTEGARELEPAQVDQ
                     AVNLVDKVRERTSIAGGHTVVALAGATGSGKSSLFNAIVGEQVARIGARRPTTSVPSA
                     AVWGTTSATELLDWLSVSSRHQVDGATRMDDADAAGSDGVGSDGLAVTGPAGSLDGLV
                     LLDLPDFDSRVDAHRIEAQRVLELVDIFVWVTDPQKYADALLHEDYVRALATHDAVTV
                     VVLNQIDRLTPEAVSACRDDLRRLLVADGLAQARVFATSARTGAGVPELVQQLANAVS
                     GANATRHRLSADVVSVAGKLRAGVADNEIDLDGRADPGLVEALSRAAGVPIVLDAVAA
                     DYRRESRQAGGWLFTRWLSNIKADPLARLRLDKTVVPMKSVGEADVRAVLGRSSIPPP
                     SPAARAAVSLATRQLADRASEGLPVRWAHAVADASATGDALTDSLDQAILHTPLRARP
                     PRWWTLVGTLQWIFGAAAITGALWLTALAVVAWLQLPDLPTPTVWAVPWPFLLLAAGV
                     VLGLVTAGLSRLAARVGARRRAAKARKRLHDAISAVALDRIVGPIAAVLARHARVREF
                     LDRAAAR"
     gene            58860..59528
                     /gene="ssb"
                     /locus_tag="PLB86_00325"
     CDS             58860..59528
                     /gene="ssb"
                     /locus_tag="PLB86_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRC63609.1"
                     /translation="MPSPFAMDGAAYPLQVEDTHMYESYVTVVGNLTADPVLRTTKAG
                     KPFATFRMASTPRRWDGRERAYVDGGTNFVSVVTFNSLAANVVASMAKGQPVIVYGRL
                     RVNMWSTAEGHSNTSVEIDAYHVGHDLMWGQSLFTKSPRAQFDTTDRLSEPEIQQALA
                     EDVDGPSDIEDEDREPDWSPINIDFTADAADPVVIEGAPLTGASSWSGANPETDAYVV
                     AHAR"
     gene            59638..>60387
                     /locus_tag="PLB86_00330"
     CDS             59638..>60387
                     /locus_tag="PLB86_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC63610.1"
                     /translation="MAEFIYTMTKARKAVGDKLILDDVTMSFFPTAKIGVVGPNGAGK
                     STILRIMAGLDQPSNGEARLSPGYTVGILLQEPPLNESKTVLGNVEEGAGEIKAKLDR
                     YNAIALEMGEPDADYEVLGDEMAKLQDEIDHANAWDLDSQLEQAMDALRCPPGDADVT
                     VLSGGERRRVALCKLLLQKPDLLLLDEPTNHLDAESVQWLEQHLAQYPGAVLAVTHDR
                     YFLDNVAQWIAEVDRGRLYPYEGNYSTYLEKK"
BASE COUNT         8895 a        20542 c        21212 g         9238 t
ORIGIN      
        1 agggagccgg cgtgcacggc atacaggtga atctcgtcgg gggagaagcg ggcggcgagt
       61 tgtccggcga cggtgagcag ggccgtggtg cggccggtcc gcggcgcgcc gacaatgccc
      121 aggtgacctt cgtgcagggg gtcccaggtc cagtcgctct gacgctggtg aaggggaaca
      181 tctctgcgcc ccaacggaac cggccatcct gaaccgccgg cgaggctcgc gacggtcaag
      241 tcacccgaca gcgggggcaa ccagggcgac ccgggcaggg gaatcgacag ctccaacacc
      301 gcgcgacggc tggctgcgac gaggtcgtcc acctgcgtcg ggccgtcaag cgcccggact
      361 gactgtggaa cggccagccc atcgatggac acaacccgca cgggcggctc gaccgctcgg
      421 cggctcgggc cggtcgcgag cgcggtctgc acctcgacga gccgggtggc tgcgctggtg
      481 aggatcgctc gccccggggt gctttcggcg atcgaggcgg cgacggggga ttcgacgaca
      541 tcgagcgagt ccacgatgtc gcggacccgt agcgcgatgc gcaggcccag gttcgcgcgc
      601 atgtcggcgg tgacgactcc gcccgggcgc tgcgtggcga tgacgaggtg cacgccgagg
      661 gatcggccaa gcgaggccac gcgtaccaaa ccggtgacaa agtccggcaa ttcctcggca
      721 agactgcgga attcgtcgac gacgatgacc agacgtccca cggtggggtc gccggggcgg
      781 catacccctt ggtaggcggc gagatccgcg acgccatgct cgccgaagac ccgctcgcgt
      841 cgtttgagct cagcatcgag gctgaccaag gcccgctcgg tcagctgatg gtcgaggtcg
      901 gtgaccaggc cgacgacgtg agggaggtcc cgacaggcgc cgaaggcggc accccccttg
      961 tagtcgacga gcacgaagct cagctcatcg gggcgattcg cggccgcgag cccggccacg
     1021 aggctgacga gcaactccga cttgccagac cccgtcgtcc ctccgaccaa cgcgtggggg
     1081 ccgtcgcgga cgaggtcgat cggcagcggc cccgaggcgg tggccccgat gggagtgagc
     1141 gtcgaccgag gactctcacg ccaacgggca gtgaggtcgg gtatgccgtc tcgctcggga
     1201 atctcgagga cgtccaacaa ggttgtgcga gagggaatca cgcccttgtg aggggttgct
     1261 gccgcgtcgc gcagcggccc cagccgctgg gcgagcagga gggcccaggc ccgcgggacc
     1321 ccatcgggtt cgaggccagt gacaggctgc tgcgcccacg tcaggctcgg ggtgccggcg
     1381 cgcaggtcga cgatctgagt gcaggcgctc ggcaggtcgg cagcgtcggc cgccacggcc
     1441 aggacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtccg gtcccacaag
     1561 ccggtcagcg ggcccctggc gaggccggcg agccacgccg ggtcggggtc ctcggccaac
     1621 cgcacgtccc gttcggcggc caaggctgcg gccaggaggg cctgcgcggt agcgagggcg
     1681 gcggcatacc ggcgctgttc tgctctggtg acccgcttcc cgttgcgacg atctgaccac
     1741 caggagccga ggagcagcaa gggccccatg aggccgaaga gcagcatcgt gggggactgc
     1801 atcaccactg ccagcaccgc ggagaagacg agcgggaggg tggcggcggc gatcggaagt
     1861 cgctgtcccg caggtgggtt cggttcctcg gggaagggga tcggtgcggc cggcccgggg
     1921 agcggcaggc gtgggctccg gctgaccagc aggtgtcctt catggtcagg gcgtgttgcc
     1981 gcggaaaccg ttgccggagt gcgcaactgc caccgagaat cgcccgtctg caaggttgcc
     2041 ccggccgcga gggtgatggg ttcacccccc acgggtatgc cgttcaccca ggtcccgttt
     2101 gttgactcca ggtcgcggac ggtgatggat cgaccggcga cggtgatctc ggcatgcagg
     2161 cgagacaagc tcgaatcacc cacgcggata ggagtattcg ctccgcgtcc gagcacgtgt
     2221 cgccccggag ggaggtcgtg gaccaggccg gtgtcaggcc cgccgacggc atggagttgc
     2281 agcaggcccg aggcggtcga ggcggtcgag gtgtcggggg aggtcggccc tgcgggacgg
     2341 atcatcagtc gggctccgtc gagcaacggc ggcagaccga gggtcgcagt ggggggaaca
     2401 ccgaccgaat caaccgacca cgtgcggctg gtgtcggtga gcgcgtgcgc ggcgtcgagc
     2461 gcgtcgagca actctgcgaa ggtcgccccg cggcgggccg cgacccgcac ctcgacggca
     2521 cggccggcca tcgggttcac cacagtcaac cgaaggttca cggaatttcc ccctgatgtg
     2581 gatgcacggc ataccggatg tgtagtcgac cggagccgac gggcgcagaa gttatccaca
     2641 gggacgtacc ggtacgagat gcgcgaatgc cgcgaatcac tatgtttgcc tcaagaaggc
     2701 tcaggggaac ggaggcgtga tggccgacgc agtgcgtcag gtcgagggtg aggttcgtga
     2761 actcatccgt cgatccgggc tcgatccggc ccgtcatccc atggagatcg accggctggt
     2821 gcgggacgcg gtcaccgact acgacgagcg ctccatgcgc agcggtatgc cgcccttggc
     2881 cgatctcgcc ggagcggcca aatccatcct cgacacggtc gccggatatg ggccgttgca
     2941 gcgctacttc gatgacccca aggtcgagga gatctgggtc aacagtcccg atcgggtatt
     3001 catcgcccgc gcggggcagt ccgagttgac caacaccatc ctcaccgctg atgaggtgcg
     3061 tgacctggtc gagcggatgt tgcggtcctc cgggcgacgg gtcgatctga gctcgccgtt
     3121 cgtcgatgcg acgttgccgg acggcagccg attgcacgtc gtcatccccg acatcacccg
     3181 cgaccactgg cacatcaatg tgcggaagtt cgtcgtgcgg gccgaccacc tggacgacct
     3241 ggtccgcctg gggaccctca cccgcccggc cgccacgttc ttggaagcgg cggtcgtctc
     3301 cgggctgaac atcctggtcg ccggaggcac gcaagcgggg aagaccaccc tcctggggtg
     3361 cctggcctcc gccattccgg cgcgggaacg cgtggtcacg tgtgaagagg tcttcgagct
     3421 caagatcccg ttgcgggacg tcgcgggaat gcaatgtcga cagccgagcc tggagggcac
     3481 cggcgctgtt ccgttgcgcc gcttggtgac tgaggcgttg cgaatgcgcc cgtcgcggat
     3541 cattgtcggc gaggttcgtc aggccgaaag ccttgacctg ctgatcgccc tcaactcagg
     3601 gctgcccggg atgtgcacct tgcacgccaa ctcggcgcgg gaagcaatca ccaagatgtg
     3661 cacgctgccg ctgctcgccg gtgagaacgt cagcccccgg ttcgtcgtgc ccacggtggc
     3721 tagttcggtc gacctcgtcg tgcacatcgc ccttgaaggt gacgggaccc gccgagtgcg
     3781 ggagatcgtc gccgtgcccg gccgggtcga gggcgacgtg gtcgaggtgg ccgacatctt
     3841 caccacccgc ggtggcgtcc tcaagcgcgc cgatggcttc ccgccccatg tcgaccgatt
     3901 cgagcgggcc ggctaccgac tgactgctct gttggcgcag gaggcctgat gtcggctctt
     3961 gcgatcggcc tgctgctcgg cgccggggtc ttctgcatct tctggtcgac ctggccgcaa
     4021 accgagcgtt cggctcccgc gcgtcgggcc agcctggctg atcgggtgcg ggaccaactc
     4081 gtccaggccg ggttcgacac catctcgccc ggccagttgc tcgccgcctg catggtggtc
     4141 ttcgtcatcg tcttcctcgg tgcctactcg atgacccggg tggtgcccat cgcgatctgc
     4201 ttcgcagcga tggcggggta cgggccgcgg gcgttggtga ccatgcgagc ccgcaaacga
     4261 cgggggcaac ttcgtgacct gtggcccgac gcggtcgaca acatcgcctc cgcggtccgg
     4321 gcgggacttg cccttcccga ggcgctgagc cagctcgccg tccgcggacc tgaggaactg
     4381 cgcccggcgt tcgccgcgtt cgcggaggac taccgcacga cggggcgctt ccacgactgc
     4441 ctcaaccggc tcaaggaccg gctcgccgac ccggtcgccg accgcctcgt ggaatcactg
     4501 cggatcgccc gggaggtcgg tggatctgac ctcgggcggg tgctgcgcac gctgtcggcc
     4561 ttcctgcgtg aagacgcccg cacccgcgcc gagttggaga cccgtcaggg gtggacggtc
     4621 aacgccgctc gcctggcagc aggtgccccc tgggttgtcc tggcgatgat gtcgatccag
     4681 caggactcgc tacgtgctta cagcagtgcc gcgggcacgg cggtcctcgc cgtcggcgca
     4741 gcgatcacgg tggtcgccta ccgcttgatg gtgcggatcg ggcgacttcc cgaggacgag
     4801 cgggtgctgc gatgagtggc gtgggcgggt ggtcgatggc cggggcggcg gcgggtgcgc
     4861 tcttctcggt cggcgtcatc ctcatcgtcc gtggggtgcc ggtcaaccga cgcccgggtc
     4921 tggatgcccg gctcgctccc tacctgcgcg acacgatcgc tccgtcccga ctcctggcga
     4981 gtatgccgcg caaccagact cgcctctcga cgttgctcgc cccgctgatg ggggatctgg
     5041 cccgccgggt cgaccggatc atgggcggct cggcctcggt gagacggcgc ctgcagcggg
     5101 ctgggctgcc tcccgacgtc gagggcttcc gtggtgagca ggtggtgtgg ggcgcgctcg
     5161 gcggagcggt cggttcggcg ctcgggctgc tcgcgatcct tgcccgcggg gcgtcggtga
     5221 tgcctgttgt ccttctcgtc gtggcgagtg tcctgctggg cgtcatcggt cgcgatcagt
     5281 ggctgtcccg ccaggctcgg ctgcgtgagc agctcatgct ggccgagttc cccacggtgg
     5341 cagagctgct cgcgctcgcg gtcagtgccg gtgagggggc aaccggcgcc ttggatcggg
     5401 tctgtcgtct ctcgcgtggt gagctctcga tcgagctcgg gcggtgtctg gccgatgcgc
     5461 gcgccggcgc caaccttccc accgcgttgc agggattggc cgaccggacc ggcctggtca
     5521 gcctgacccg cttcgtcgac ggcatggtca tcgctgccga gcgaggaacg cccttggctg
     5581 aggtgcttcg cgcccaggcg caagacgttc gggaagccgg tcgtcgcgcg gtgatggagg
     5641 ccgggggccg caaggagatc gcgatgatga tcccggtcgt cttcctcgtc ctgccggtga
     5701 cggtgctctt cgccgtattc ccgggcttcg ccttccttcg attgaccatg tgaggccgcc
     5761 gatgacgagg cgtcccaccc tgatccccgc caaccgcttg gcggtcgaac caccaaccag
     5821 tcccatccac cacgtcacca cgggggccct gcaatgacac ctgtacacac ggcatacctt
     5881 cggactgcga gcggcttggg cgcgctctgg tcagccgcca accgcgccgc tcggcgcgcc
     5941 aggcgggacc ggggcgacgt ccccggctgg gtcctcatca ccgtcatgac agcgggattg
     6001 gtctcggggc tctggatcgt cgccggccct gcgctggtcg acctgttcca gagttccctg
     6061 tcgagtgtca ccggcaagaa gtagcccgat ggccgcggtg gcggcgaccc ggccgggtga
     6121 gcgtggctcg gcggtcgccg agttcgtcat ggtctctacg ctcgtcctgg tcctcggcat
     6181 gggggtcttc caactgggcc tcgtcctgca cgtgcgcaac accctcatcg cctgcgccgc
     6241 cgaaggagcc cggctcggcg ctcgggccga cgtgcccacg tcggacgcca gtgagcgaac
     6301 tggcgcgctc gtgaccgaca ccctgtcggc ggcatacgcc caggacatct cgacgcggcg
     6361 gacggtgacc gacgcgggag tccgagtcgt cgaggtcacc gtcgttgccc ccctgcccgt
     6421 gatcggcctg atcgggccgg gcgggtcgat gacggtggtc gggcgggcgt tcgacgagcg
     6481 acaggtgatc gagccatgac caggctcacg gggctcgttc ggtgggtgcg gctgcgggtc
     6541 ggtcgccccg acgaggggcg agcaatcgtc gagttcgtct tcctcggggt gctgttgctg
     6601 gtgccgctca tctaccttgt catgacgctg gcggtgctgc aggcggcggc atactcagcg
     6661 acaacggcgg ctcgcgaggc gggtcgcgcc ttcacgacgg ccccggcgga gagccaaggt
     6721 atgccgcgcg cccgggccgc ggcccaggtc gccttcgaag acttcgggtt cctgcccgag
     6781 gagacttcgg tggcgatcac gtgcgacggc tctccctgtc tgcgcccgga cgcgcgggtc
     6841 agcgtgacga cgtcgatccg ggtgcgactg ccctaccttc cggcgttcct cggcgatgcc
     6901 gtccccgccg cgattcccgt gtcggccacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnc tcatcctcgg catggtcatg ctcgcgatgc tgctgatcgt cgggggagtg
     7081 gatgtcactg ccgcgcaact ggctcgggtg cggttggtcg atgcggccga cgcagcggcg
     7141 ttggatgcgg ctgacgcgtt ggacgaggcg acggcatacc gcagcgggtt gtcggacgcg
     7201 gtggtgctca gttcggcgac ggtgaaggag gctgcggcgg catacctctc ggtgcggccc
     7261 aagcccgagg gggtgcgggc gtgggggatc gcgccgggga cgggcgctca gggaggcgac
     7321 accgcggtcg tcgtcctgga tgcgaccgtc gagttgccga tgaccggcgg actgctcgcg
     7381 gcgctcggca aggagatcgc ggtgcacgtc gaggccaagg cccgcgcccc gctcctgcct
     7441 gtgccctaga tacgacgtgc gccgcggcgc ccgaggccgg tcaaccggcg ctgtgcggag
     7501 tgaaggcgac cctctggtgt ggccaacact ccgcacaggc cacctcaggg gcggcatacc
     7561 tcaaccaggc tcagcgcagc aggtcggcga cctcggcgga ggcgtagaag ggctcaagct
     7621 gggcacggag cagccgttcg aggtctccgc tggcgcgcag ggtgtcgacc acgaacgaag
     7681 ccagcggcac gaggtgggcg atgacctcgt cgcgcggcca ttcgagttgg tcgagcagct
     7741 cagccagggt gtagcccccg aaccactcga agagggcgtc gaccaccagc gtggcgacgc
     7801 gggcggcgtg ctcggtgccg agggcggtca cggcgagttc gtgggcggcg tcgaccacgc
     7861 cggagacctc cgcgtgcgcg gcatacgctc cgagtccgat gacccgctcg ccggaggtct
     7921 ggtcccagac ctgcaggatc gcctcgcggg tcgtggggtc gcgcagggtc tcgacgaaga
     7981 ttcggttgag gcgccgcacc gcgaacgtgc cgcccttgcc gaccgcgtcg ccgatcagcc
     8041 cttcgaactg ccggtccgca gcaccccgca gccgcgatgc cgtgctcgtc ccgaaggaca
     8101 ccagcggccc cagcccgggc accttggatg cgacagcctg gttggcctgg atcacctcgc
     8161 tggcgatccg gcccatgaac cgcgacgcga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn ngtcaactcc agcacggagg tcacgaggac ctccacctgc tcccggtcga
     8341 ccgtctcgcc gacggggaag ggttgccacg gaccgtcgtg agcgacctgg gtgcccagct
     8401 cgacgatgcc gctgacggcc gcgctgcccg gcacgttcgc catggcccgc accacgacgg
     8461 cggcgacggc ttcccggtcg atcaggtcgg cgagttggtg cggcccggcg ctgtccagga
     8521 ggctctcgac gagcccagca accgtggact cgagccggtc gccggtgaac cgatcgaggt
     8581 ggtaggccac ctgggcgtcg agcagccgct gggcgagggt gtctgctcgc gacgcacggg
     8641 gaggcatggc cccacgctag accgcgcccg cggcgagcac acggcatacc aatggtcggg
     8701 tgggatgcgg cacctgagca cgggccgcgc ccgcctcgcc gaccaatggc cttcggtgtg
     8761 cgcaggggta tgccggtcgc ccgcctccca gccccggtgc ggcgcgggtg cggctccgac
     8821 gtggggcacg atcggtgagc ggcgcggcat acagggggcc tcgcgtaggc ttgaccctcg
     8881 tggctgtcga cttcggagaa gagatcaagc acctgcgcgc gacgatggat tccgtgcgcg
     8941 aggtgaccaa cctcgatgct ctgacgaccg agatcgcgcg gctggaggcg gaggcgtcgg
     9001 tccccaacct ctgggacgac cccgaccggg cgcagcaggt gacctctgcg ctgtcccgcg
     9061 ccaactccga gcgcgaacgg gtgaccggga tggacagccg ggtcgaagac ctcgaggtgc
     9121 tcgtcgagat gggcaccgag gagggcgacg ccgacaccct cgccgaggcc gagcgcgatc
     9181 tggccaagct taagaaggac gtcggtgagc ttgaggtccg caccctcctc aacggtgagt
     9241 gggacgcccg cgaggccgtc gtcacgatcc gctcgggcgc tgggggagtg gacgcggccg
     9301 acttcgccga gatgctcatg cggatgtacc tgcgctgggc cgagcggcac aactacccga
     9361 caacggtgct ggacacctcc tacgccgagg aggctggcct caagtcggcg accttcgagg
     9421 tcaaggtgcc ctatgccttc ggcaacctct cggtcgaggc gggcacccac cggctggtgc
     9481 ggatcagccc gttcgacaac caggggcgcc ggcagacgtc gttcgcagcg gtcgaggtgg
     9541 tcccgctcat cgagcagacc gaccacatcg acatccccga gaacgagctc aagatcgacg
     9601 tgttccgctc gtccggcccc ggtgggcaga gcgtcaacac caccgactcg gcggtccgga
     9661 tgacccacat ccccacgggc atcgtcgtct cgatgcagaa cgagaagtcc cagatccaga
     9721 accgcgccgc cgccctgcgc gtcctgcagt cacgcctgct gctgcagaag aaggccgagg
     9781 aggccgccac ccgcaaggag atggccggcg acgtcaaggc cagctggggt gaccagatgc
     9841 gttcctatgt cctgcagccc tatcagatgg tcaaggacct gcgcaccgac cacgaggtcg
     9901 gcaacccgtc ggcggtcttc gacggcgaca tcgacgactt catcgaggcc ggcatccgct
     9961 ggaagcgcaa cctggagaag ggcaacggct gaccgcggcc gtaagtctca gcctccgctc
    10021 gccgatcagc ccgcacgcac cactcagccc gagtgggtcg tcactccgta tgccgtgcgg
    10081 ttggccagcg cccagcgccc cagcgcctgc gcggcgacac cagagaccag gacgatcagg
    10141 cccatcgcga cccaccccag gcggccgccc tggatgcaca gcagcgtgac caccggcggc
    10201 ccgacgacgt tcatcagcga gaagcccagg ctcgcgaacc cctgatattg cccctgccgt
    10261 tcgtgagggg ccagccccat ctgcagcgcc cactggccgc cggagccgat catctcgccg
    10321 acgacatgga cgctggcgcc cgcgagcagc acggccacag ccacccatgc cgagctgccg
    10381 tcggccagcc cgatgagggc gaacccgacc gcgatgagcg cgccgccgcg caccaacgcc
    10441 cgccctccag tggcgacgga gtcggcggag gtggacaggc ggacctgcag cgaggcgacc
    10501 gcgaccgtat tgaccagcag gagcactgcg accatcactt ggggcgcctg ggtccgctgc
    10561 gacacgaaca gggccagacc caactcggcg gcgacgaagt gcacggagaa catcgacgtg
    10621 ctcagcacga ctgcgacgaa cggcaggtcg cgcagcaccc cgagccgcgg ctcaccgtcc
    10681 ggtcggcggt agggcggcag gtgggtcagc cgggtggtgt tccacgcagc gaagccgcac
    10741 agcacgccgg ccatcacgaa gacgctcacg tatgccgcgc gggtgtccac gacgagcgcc
    10801 gccccgccca cgaccgagcc cagcgcgatg ccgctgttgg tcacggcacg cagataggcc
    10861 ttgaactgca cgcctcgccc gccgatcgcc acctgggcgg tgaggccatt gcggaccgcg
    10921 ttggcggcgg aggccagggc ggcctcgacc gccagcagca gggtgagcac ccacggcgag
    10981 gttgccagca cgggcaacgg cgtcgcgacc gcgagggcga gcatgatcac gttgagcacc
    11041 tcgcgggggc cgcgggtgtc gcccagatgc ccagcgggaa gggccgcgat catcgcggcc
    11101 gcggctgcca ccgagacggc cagcgccacc tccccgacag tgaaacccag gtggcgagtg
    11161 aagtagatgg ccgaggtcgt catcaccgca ccgctgccga agcgggtcgc gaacgctccg
    11221 atggccagga cgcgcagttg gggatcgagg ggtatgccgc gtcgcgtcag cgcctcaccc
    11281 tcgcctgcgg cggccatcgg gttccccctc caagtatctt gacatcaaga tacttcccta
    11341 cgcccgacga gtgcaaaccg ggcgcaatcg ggtacaaccc ggccgcggcc agagggggga
    11401 gggcgcgagt cggggccttg cggcataccc ctcgcgccac gccagccacg tgcataactg
    11461 tggtttctcc agtcactcaa ctaccctcgg accacaccag ggctcgcgca ggccctggcc
    11521 ccaggctgcc ctgaccggag aaaccgacgc atgatcctgt ttgacgccgt gaccaagacc
    11581 taccccaagg ccacccgccc ggccttgtcc gaggtctccc ttgaggtcga gcgaggtgag
    11641 ttcgtcttca tcgtcgggag ttctggctcg ggcaagtcga cgttgttgcg cctggccatt
    11701 cgcgaggaac gggcgacgtc gggcgccgtc ctcgtcgctg gccgcgagct gcgcacgctg
    11761 cccgatcgtc gggtgccgat gctgcgccgc gagatcggga cggtcttcca ggacttccga
    11821 ctgctgccca acaagagcgt cttccagaac gtcgccttcg ccctgcaggt catcggcaag
    11881 tcgcgcgccc tcatccgcca gaccgttccc gagacgctgc gcgtcgttgg gcttgagggc
    11941 aaggagaagc ggttgcccca ccagctctct ggtggtgagc agcagcgggt ggccattgct
    12001 cgggccatcg tcaacaagcc gaccatcctg ttggccgacg agccgaccgg caacctcgac
    12061 ccggccacca gtcgcgcgat cgtcgagttg ctcgaccgga tcaaccgctc cggcaccacg
    12121 gtcgtcgtgg ctacccacga ccacgagatg gttaacgagt tccgccgccg ggtcatcgag
    12181 ctcgacgccg ggaagttggt gcgcgacgag gccgacggcg actacggcaa gacgacctcg
    12241 gcaatccccg tcgttcgaat cgatgagggc gactcggacg ccgaggatgg gacccccgat
    12301 gccgggtcgg ccggagggga gcgctgatgc gggccggatt cgtcctcggt gaattgggca
    12361 ccgggctgcg tcggaatctg tcgatggccg tgtcggtcat cctcgtcacg atgatctcga
    12421 tgtacctgct cggactcggg ctcctggccc agcgccaggt cgacaccatg aaggactact
    12481 ggtacgaccg cgtgcaggtc tcgatcttcc tgtgcgtgga gggctcgcag tatgccgcgt
    12541 gcagcggtaa ggcgaccacc cccacccagc gtgagcagat caaggcccag ctcgagcaga
    12601 tgaagcctcc ggtcaaggag atcttctacg aaagccaggc cgacgcgttc aagcggttca
    12661 aggagcagtt caagaactcc ccgttggccg agcaggcgac cgaacgggcc ttcgccgact
    12721 ccttccgggt gtcgttgacc gacgcaggga aatacgacgt catcgtgtcg tcgttctccg
    12781 gtgccccagg agtcgggcgg gtccaggacc agaagcagtt gctcgaccga ttcttcgcgt
    12841 tcatgaacgc cgtgtcgtgg ggggcgatcg ccctctcggc gctcatggtc ctgtgcgccg
    12901 tcctgctcat ggccacgacc attcgccaga cggcattttc gcggcggcga gagaccggga
    12961 tcatgcggct ggtcggtgcg tccaacgcga cgatccaact gcccttcgtg ctcgagacgc
    13021 tcgtctcggc cctcgtcgga gcctcgttgg cgatgggcct gttgtgggcg accgttagtt
    13081 atggcatcgg gggatacctc agcaaggcgt tgatggacac tgcgttcatc gggatgacgg
    13141 atgtcttcgc cgtggctcct tgggtggtcg gcggggtggc gatcgtcgcg gttctgacgt
    13201 catggttcac cctcaggcgc taccttcgag tgtgacgggg gtcgggggat gcaaggtcga
    13261 ttggtcaggt ttcacaggtg agcccgctca tctgtgccga ccgagtgtgg taccaaaggg
    13321 actgatgatt ctccagagat tgatgcgacg ggtgttgact tcaccccccg gccgccgccc
    13381 aggcttgcgg gctggcgccg ccggtctcgt cgtgctgtcc ttctctgccg tgcttctggc
    13441 ccctccgtcg gctgctgata ccggggatga gaagcgtgcc gccgacgccc aggtcgccga
    13501 cctggaggag caactgagcg agacctccgc cgagttggcg caggcctata cggcgctcac
    13561 tcagactcag gcgcagttgc ccgccgcccg ggctcggttg gcctcggcgc agtccgcggc
    13621 gagagcagcg gccaaacaca acgcagaggt ggcggcagcc ctggcggtgg cgcaggcgaa
    13681 tgccgcgcgc gccgaggaag cggtggcccg caacacggcc acccgcgatc aaactcaacg
    13741 cacgttggat gccttcgccg cagacctgtt ccagggcggc tcggtgggcc agctgtccgt
    13801 ggctctgggc gccaccagcg ccgacgactt cgccacgcga ctcgtcctgg ccgacacggt
    13861 gacggcgatg accaaccagg ctctcaacga cctcgccgca gctaaggccg acggggacgc
    13921 caacggcgcc tacctggctg cggtgcaggt cgaggtcacc gacctcaagc ggcaggcgga
    13981 gaacgccttg gccgccgcgg aagcctcgcg aaacgaggcg caggcagcaa gttcggccct
    14041 cgacgcgctc gtcgcccaac agagtgggta tgccgcctcg gtcgaggccc agcgcgccaa
    14101 cgaggcgagc caattggccg aggcgcaggc cgaacaacag cgcctgcaag ccctgctcat
    14161 cgagcaggcc cggatcgcgc gggagcgtga ggcggcccgc aaggccgcgg aagctgctgc
    14221 ccagaaggcc gccgaggaag ccgctcgcaa gaacggcacg gcggtcccgc cgcctcccgt
    14281 cgcgcaaccc gaccaggcct ccggcttcct cagccggccg atcaccggtg gtcgcaccag
    14341 ctcggagttc ggcatgcgtt tccacccgat cttccaggtc tggcggttgc acaccggcct
    14401 cgatttcgcc aacggctgca gttcgcccgt gtatgccgcc gcgcccggca cgatcatcag
    14461 ggccgggtgg gccggtgggc gaggcaacca gatcgtcatc gaccacggga tccgccgggg
    14521 tgtcgacctg gccacgacct acaaccacct cacgtccttc gtccagcgca gcggcaccgt
    14581 gtcgcgcggt cagctcattg gctatacggg gacgaccggg aactccaccg gctgccacct
    14641 gcacttcgag acgttggaag acggccagtt cgtcaatccg cgctcttggt tgtaggcgtg
    14701 cttaaagcct tcccggccgc aggtgccgac ggggtagcgt tggctctccg gtaggtgaca
    14761 gggaggaccc actcacgtgg tcaaggaaag tggccgaaag gtcgtcgcga gcaaccgcaa
    14821 ggcccgccac gactaccaca tcgaggacac cttcgaggcg ggcatctccc tcatgggcac
    14881 cgaggtgaag gcgttgcgtg ccgggcgctg ctcactggtc gacggcttcg cccagttcac
    14941 cggcgacgag ttgtggctcg agggtgtcca catccccgaa tacagccaag gcacctggac
    15001 caaccacgcc cctcggcgcc gccgaaagct cctcctgcac cgcgaggaac tcaccaagat
    15061 cgagtcgaag atccgcgaga agggccgcac gatcgtcccc ctcgccgtct acttcaagga
    15121 cggccgcgcc aaggtcgaga tcgccctggc caccggcaag cggcaatacg acaaacgcca
    15181 ggcccttcgc gaacgccagg acaagcgcga agccgaccgc gccatgcact cccggagcga
    15241 gcggtgagcc acccggctcg ctcgcatcga caccgtcggg ggtatgccgc gcggctcgcc
    15301 ctgctggtgg gcgccgcgct cactctgctc ggcgtcgcgg cagccccagc cgccttggcc
    15361 cagacaccgg ctcagagttc aacgacggcc gcggtcgcac cctggtccga cctctcggcc
    15421 gctcccgcgc tggccgctct ggcgacggct gatcggatgc ccgctttcga ctcggcgttc
    15481 gtcgtcgaca aggacggcgg gatcacggtc accgaaacga tccagtacag cttcgactcc
    15541 tccggcggcg cccggcacgg catctaccgg gcgatcactg tgcggcaggg ggtcgacggg
    15601 cgcaccgacg tctatcgcta cctcagcatg acgggggtca gcgtctccag cccgacgggc
    15661 gccgacgcca cggtccagtt ggtcgacaac ggccccgatg tgacgatccg gatcggctcc
    15721 gcctccaaga cggtgagcgg cacccagacc tacgtcgtgc gctatcacct ggccaacgtg
    15781 ctcaacccgt tcaccgacac ccagacggcc gagttctact acaacgtcat caaggacgac
    15841 acggtcgcca agggtgcggt caccgtgtcg gtgacggctc cggtcgccgc gaccgacgtg
    15901 cggtgcactc gcggggtcgg cgcagcctgc gactcggcca ccggtggcgc cacggcacgc
    15961 ttcgccgtgg gcagcctggc ggcgggggag aacctcacca tcgacacgcg ctatcccctg
    16021 tccggtttct cgaagatcgc gccggacatc cgcaccggca ccgccggggc tcccggctcg
    16081 ggcctggatg agggtgtcgc gaaggtcgcc tcgctcgcgt cggtggccgg tggcgtcctc
    16141 gcccccatcg tcgcgctcgt cgtcatgggg atgctcgtcg ccacccgtgg ccgggacgag
    16201 tggtatgccg ggctgacccc aggtctgacg ccgggcctcg ggcaggggcc gatcgcggac
    16261 ggcatacccc ctggtgggtc cgcgcccacg gtgcgaggga agactccgac gatcgcggtg
    16321 cagttcaacc cgccgccggg agtgcaaccg ggcctggtcg ggacggtgat cgacgagtct
    16381 gccgacacgc tcgatgtgtc ggcgacagtc atcgacctcg cggtccgggg ctacctgcgc
    16441 atcgaggagg tcgagggcgg ggggatgttc gcccgcaccg actggcggtt gacggccctg
    16501 tccccgacga cggcgcatga gttgcgcccc tacgagcaga gcgtgctcgt cggattgttc
    16561 cagcagggca gcccggtgct gttgtcggac ctgaagaacc acttcgcgaa gacgctggcc
    16621 gcgaccaagg acctgatgta cgacgaggtg ctctcgcgcg gttggttccg caagtcaccg
    16681 cagagccagc ggacggcctg gcgtggtttc ggcttcttcg tcatgggcgc cgggatcgtc
    16741 acgatcttcg cctacggctc gatctcgcgg gccatcgaca tctccagcgg gctgcccctg
    16801 ccgatcccct cgggttgggt gctgggcgcc gggttgctgt tggcgggttt cgtcatccag
    16861 ttgctggggg cccggatggc cgccaagacc gccgccgggt cggccgtgct cgcccaatcg
    16921 ctggggttcc gccaatacct cgtgaccgcc gaggcgaacc agatcaggtg ggaggaggca
    16981 caagacgtct tctcgcgcta cctgcccttc gccatcgtct tcggggtcgc cgaccgttgg
    17041 gccaagacgt tccaggaggt cgcggccgct gctgcggctg ccggccaggt cgtcatcatg
    17101 cccgactggt acatctacca cggcacgttg ttccccgatt tcgggagcat cgtcgacggg
    17161 gtcgactcgt tctcgacgac cgccagtggg acgttccagt cgacgcccgg ctcgtccggc
    17221 gggtccgggt tcggcagttc gggcgggtcg ttctccggtg gcggggtcgg cggctcgtcc
    17281 tccggctcgt ggtgagccgg aggacctgac ccgacgccgg cgggctagcc cgccgaggcc
    17341 tcgatctcgg gagccttggg tgcgggcggt tgaccgacct ggacgacgag gatgaggtct
    17401 ccgccgtcga cctgggtgtg tccgccgatg gccagccgct cgacgacgcc gccgaccggc
    17461 gtggtgatcg aggcctccat cttcatggcc tcgatggtgg ccacgaccga gccggccgcg
    17521 acggtgtcgc cctcgcggac cgcaggcgtc accatgccgg cgaagggcgc cgcgatgtgg
    17581 cccttgatcg ccgggtcggc gcgctcggcc tggcggacgt cgaccttgac cgacgtgtcg
    17641 cggatctgca ccggccgcag ttggccgttg agggtgcaca tcaccgtccg gaaaccccgc
    17701 tcgtcgggct cggacaccgc ctgcagcgag acgatgatcc gcttgccggg ctcgatgtcg
    17761 atgctgtgtt cctcgccggg cacgaggccg tgcaggaact cgaccgtccc gacgatcgac
    17821 aggtcggagt aggcctcgcg ggaggcgagg aactccttgg tcggcccagg gaacagcagc
    17881 cggttgaggg tggcccgcgg gctcacggcg agcgccgcca ggtcgtcggg tgccaactcg
    17941 gtctcggccc gcttgatcgt gcgcccggcc agcgccttgg tccggaacgg ctcgggccag
    18001 ccaccgggcg ggtcacccag ctcgccgccg aggaagccga tgaccgaatc ggggatgtcg
    18061 tatgccgtgg ggtcggcttc gaaggcagcc gggtcggcac ccgctccgac gagggccagg
    18121 gcgaggtcac cgaccacctt ggaggacggc gtcaccttga cgatgttgcc gaggatcttg
    18181 ttggccgcgg catacatgtc ctcgatggcc tcgaagcggt cgccgagccc gagggcgatc
    18241 gcctgctggc gcaggttgga cagctgaccg ccggggatct cgtgggtgta gacccggccg
    18301 gtgggggagg ggaggcccga ctcgaacggc cggtagagcg tgcgcatcgc ctcccaatag
    18361 ggctcgagcg aacagacggc cgcgaggtcg agcccggtcg cgcgttcggt cgcattggtc
    18421 gcggcgacca gggccgacat cggcggctgg ctcgtcgtgc cggccatcga cgacgaggcg
    18481 acgtcgacgg cgtcgacacc ggcatcgatc gccgcgagga gcgtcgcgag ttgaccgccg
    18541 gccgtgtcgt gcgtgtgcag gtggaccggc aggtcgaacc gctcgcgcag cgccgtcacc
    18601 aggcgacggg ccgcgggggc gcgcagcagc ccggccatgt ccttgatggc gatgatgtgc
    18661 gccccggcgg cgacgatcgc ctcggccagg tgcaggtagt agtccagcgt gtagagccgc
    18721 tcgccggggt cgttgaggtc ggaggtgtag cacagggcga cctcggcgac ggcggtgccg
    18781 gtggcgcgca ccgcgtcgat ggccggacgc atctgggaca catcgttgag tgagtcgaag
    18841 atccggaaga tgtcgatgcc ggtcgctgcc gcctcggcga cgaacgcgtc ggtcaccgtc
    18901 gtcgggtagg gcgtgtagcc gacggtgttg cggccacgca gcaacatctg caacggcagg
    18961 ttgggcatgg cggcacgcaa tgcggccagg cgatcccacg ggtcttcggc gaggaagcgc
    19021 agggccacgt cgtaggtcgc gccgccccag gcctcgaccg agagcaactg cggggtcaac
    19081 gccgcgacgt gcggtgcgac atgcagcagg tcgcgggtgc gcacccgggt ggcgagcagc
    19141 gactggtggg catcgcggaa ggtcgtgtcg gtgaccgcca cgggcacctg ggcccgcaac
    19201 gcggcggcga accgctcggg gcccaactcc agcagtcggt cgcgcgcacc ggcgggcggg
    19261 gcggcatacc gatcgagcgt ggggagtttc gcgcgcggtg agatgcgggt cgcggcgggg
    19321 ccgtgcggtt ggttgaccgt cacatcggcg aggtaggtga gcaacttcgt gccgcggtcg
    19381 tgcggcgtcc gagcggtgcg caggtgcggc cgctcgtcga tgaacgaggt ggtcgcgcgg
    19441 ccggcgacga agtcgggctc ctcgagaacc ccttgcagga aggggatatt ggtggaaacc
    19501 ccacggatgc ggaactcggc caacgcccgt cgggcgcggg tcaccgcggt cgcgaaatcg
    19561 cgcccgcggc aggtgagctt gacgagcatc gagtcgaagt gcgggctcac ctgagcgccg
    19621 acgaagaccg tgccaccgtc cagtcgcacg ccggagccac ccgcgctgcg gtatgccgtg
    19681 atctcgccgg tgtcggggcg gaactcgttc gccgggtcct cggtcgtaat gcggcactgc
    19741 agggcggccc cgcgcagggt gatcgtgtcc tggctgatcc cgaggtccgg gagggtctcg
    19801 cccgaggcga tccgcagttg cgaggccacc aggtcgatgt cggttacttc ctcggtgacc
    19861 gtgtgctcga cctggatgcg cgggttcatc tcgatgaaga cgtagcggcc gttctcggcg
    19921 aggaggaact ccaccgtgcc ggcgttgacg taaccgatct cgcgggcgaa cgcgacggcg
    19981 tcggcacaca tctggtcgcg caacgccggg tcgaggttgg gcgcaggagc gacctcgacg
    20041 accttctggt ggcggcgttg gaccgaacag tcgcgctcga agaggtgcac gacgttgccg
    20101 accgcatcgg cgaggatctg cacctcgatg tggcgggggc ggatcaccgc ctcctcgatg
    20161 aagaccgtcg ggtcgccgaa ggccgactcg gcttcgcgca tcgccgcggc cagcgcgccg
    20221 cgcaggtctg cgggagcgtc gacccgacgc atgccgcggc cgccgccacc ggcgaccgcc
    20281 ttgacgaaca cggggaaccc gatctcctcg gcgcccgcga cgagggcctc gacatcggtg
    20341 ccgggcgggc aaccgcgcag cgtcggcagg ccggcgcgcc gagccgcctc gatggcccgc
    20401 gacttgttgc cggtgaggtg caggaccgca gcaggcggac cgacgaagac cacgccggag
    20461 ctctcgcacg cctcggcgag gtcggggttc tcggacagga agccgtatcc gggatagacc
    20521 gcctgggcgc cgcacgcgac ggccacctcg acgatggcct gcggattgag gtaggcacgg
    20581 acgggatggc ccggctcacc gatctcgtag gcctcgtcgg ccttgagccg atgctcgctg
    20641 gaccggtcct catgggggaa caccgcgacc gtcttcgcgc ccaactcggt cgctgcccgg
    20701 aacgcgcgga ccgcgatctc tccgcggttg gccaccaaga ccttgccgaa catgtcagcc
    20761 tccgattcct cgtgccagtt gcggcagcgt agccatcagc cgcgacgtcc cgccgcgcgg
    20821 tttcgcctgt cagactcttg cgggtgacct cgaccctccc gacgcttgcg gcggtgcagc
    20881 gacggaccat cgcgaccctg ctgtccaccc aagtgctcgg tggcctcggg gttggtgctg
    20941 cgatctccgt caacgccatg ctggccaagg atgtgtccgg tgtcgagtcg ttggcgggtt
    21001 tggcccagac cgcggccgtc ctcgggaccg ctctggtcac gctcctgatc gcgcgggtga
    21061 tggacgacca cggccgacgc atcgggctga cgctcggcta cgccctgggg ttcatcggca
    21121 cggcgctgtc catcgtggcc ggcgtcaccc gggtgttccc gctgcttctc gtcggggcgg
    21181 tcttcctcgg cgcgaccacg gcggccaaca accagagccg gtatgccgcg accgaccttt
    21241 cgctcccggc ccaccgcggg cgggacctct cgctcgtggt gtgggccacg acggtgggct
    21301 cggtcctggg ccccaacctg tccgggccgg gcaaggtcgt cgcttcggcc gtcggactgc
    21361 ccccactgac gggggcctac ttgttctccg gcgtcggcat gctcgccgcg atggcggtca
    21421 tgttcttccg gttgcgcccc gacccgctcg tgctggccag ggccgaacac ctcaaggaac
    21481 tccctgccgg gacggcgccc acggcccggg cctccggacg agccctgctg cgggagaacc
    21541 gactgctgcg tctcgcggcg atcggcatga gcctgagcca cgcggtcatg gtctcggtca
    21601 tggtgatgac cccgatccac atggatcacg gccacgcctc gttggagatc atcggcctgg
    21661 tcatcagcat ccacatcctc gggatgttcg tcttctcgcc cgccgtcggc atggccgtcg
    21721 acaggtgggg gagcgccccg atcctcgccg cgggcggtgt cgtgctcctc gtggcgctcg
    21781 tgctggccgg ctcgtcccac gccggtgcct cctggacgct cgggctcggc ttgttcctcc
    21841 tcggggtcgg ctggtccctc ggcacgatcg cctgctcgac cctggtcacc gcggcgtcga
    21901 cgccgcacac gcgccccgcg atccagggcg tcaccgacct gctcaccagc ctggcgggcg
    21961 cgttgggcgg ggcgatcgct gggctcgtcg tggggctggc gggatactcg gccctcaaca
    22021 tcggcgcggc cgtcctcgcc gtgggcgtcg tcacgacggc ggtcgccgtg gggcggcaga
    22081 ccgaatctgc cgactaaggt agggacgatc cccacgaccg ccacgacgag tggagcgcga
    22141 tgccctcctc cccgggtcac gaccgcgccc acgtcgcgcc cgccggctgg acggggcgaa
    22201 tccggcggga gttcgacgat ctccgttacg cctggggcga cccggtcacc cggtgggggg
    22261 cgctgggcag cctgctcatc ctcatcggtg cctacggccc gtcgacgatg ccgaactcga
    22321 acttctggcg ggacctgccc gtcatcggcc tcatgcagac gacgctcccc ggccggatcc
    22381 tggcgactgg ctccatgctc atcggggtcg tcatcctgct ggacgcgtgg ttgcggctgc
    22441 ggccgtccaa gggacaccgc ccgctgaccc gcggcaccgt gtggtggtgg tcgctgccgc
    22501 tgctcctctc gccgccgctg ctgtccttcg acgcattctc gtatgccgcc caaggcgagc
    22561 tcgtctcgcg tggcttcgac ccctacaagg tcggcccggg ggtgctgggc gacgccttca
    22621 ccagccaggt cgaccagatg tggcgggaca ccccggcccc ctacggcccg ctgtcgctgc
    22681 aactgcagca cctcatcgtc gtcgtcaccg gccaccagcc ctacctgtcg gcgctgctcg
    22741 tgcggatccc cgcgttcatc ggggtgctgt tgctcgcggt ctatctgccg cggctggccc
    22801 gccggatcgg ttacgaccga gagacgacga tctggttggc cgtgctcaac ccgttggtcc
    22861 tgctgcacct tgtcggcgga gcccacaacg acgccctcat gatcggcctc atgatgtggg
    22921 ggctctgggt ggccgctaag acgcactggg tgcccgcagc ggtgatcatt gcggcggcag
    22981 cgtcggtgaa acagcccgcg gccgcagccg tgctcgccgt cgccgccttg cacgcttggc
    23041 gggtcggggg ctggcgcgag caaccgagca acgccacagt cgcccggtat gccgtcggcg
    23101 tcgtcggcgt gttcctcgcg gccttcgtgg ccattggact gctcaccggg ctcggcttcg
    23161 gctggatcac cgccctgggc gtgcctaacg ccgtgcgcag cttcctctcg cccgtcacga
    23221 ccatcgccca aggcctggac tgggtcctcg tcagcctcgg ggtcatcccc gacgccgacc
    23281 tcgtcgtccc gatcgcgcag cgggtcggtc aggtcgtcgg cctgctcgtc gtcatctgga
    23341 tggtgttcaa gttcaaggcg cggcacccgg tccgcacgct cgggtggatc ctgctcgtcc
    23401 tggtcttcac cggccccgtc gtccacccct ggtacatcct ctggggcggc ctgctcttcg
    23461 ccatgaccga gtccggccaa ctggcccgac ggatcgtcgt ctgggcgacc tgtggtctgg
    23521 cgatgtactc cgtcgtcgac gctgcctggc gcagttcagt cgtcgccgtc ggggtcaccg
    23581 cagcgctggc cctggtgtgg atcaccaccg gccatgaccg ggacctcatc cgcgacaagg
    23641 acttcgccaa gtcgcgtcgg accccggcct gagcttgcga tctggccccg cccgggaatg
    23701 gagtcgaccc cggtagccgt tgcacggcat actgaaggtt cttgacaact gcacagcgtg
    23761 acgtggacac ccctcacggg ggtgatcggt ttcgacggtg atcgtcgtca caggagaagc
    23821 gggccgagga cgcacggtta tctcgttaac gatccgcgca aaccaatagg tgccaactca
    23881 aagcgcaccg acttcgccct cgccgcctga gcgagcctcg aagtccgtca gcctgagata
    23941 gtttccgact cagtgcctgg cgtcagctag gaaactcacc gcgccgtcat gtcggggggc
    24001 ggcgtgggac atctactcga ctgggcctgt cagggaacgc gtgcgcgcga gccctggggc
    24061 cgagaaactc catagcgcac tgcgcccgga gaagtcctgg gatcacgtca tcggacgcgg
    24121 gttcgattcc cgccacctcc actactcggc atcggtcagc agcccgtatg ccgtgtggac
    24181 acgtcacgct ggacagcgcg aaaggcgccc cccgggcttt ccgggtgggc gcctttcgtg
    24241 ttgcggcgag tgttggctgg ggcaggtcag ctggtgcgcg tcagacgctg aaggagcgct
    24301 gcgggccggg accccacggg gtcgccttgc cgaggtcgtc gatccggacg atgtccttgc
    24361 ccatcggcac cagcgacacc gggatgagct tgacgttggc caccgccagc gggatgccga
    24421 tgatggtcac ggcctgcgcg atggcggtcg tgaggtgtcc gagggccagc caccagccga
    24481 agcagacgat ccagacgatg ttgccgatcg tcgaagccgg cccggcgtcg ggcttgtcga
    24541 cgatggtgcg cccgaacggc cacagggcgt agttggccat ccggaacgac gcgatccccc
    24601 acgggatggt gacgatcagc agacaggcca gaacgccggc ggcgaggtag gtcagccaga
    24661 gccagaagcc ggcgaagacg acccagatga tgttgaggat ggtgcgcacg ggcgcttctt
    24721 tccggtggtg cgaggtgttg ctctggtatg ccgtgaacgt tgctcaggcc tgggaggttc
    24781 cggcaccctg cggctcggcc gcgccgtcgt gcccagcctc gtgcccagtc tcatgcccgg
    24841 cctcgtgctc ggcgatctgg cgacgcacgt cggtcatgtc caaggccttg acctggccga
    24901 cgaggtcctc gaagaccggg gccgagaggg cgccgggctg cgagaacacg aggatgccct
    24961 cgcggaaggc catgatcgtc gggatcgacg tgatctccag ggctccggcc aaggcctgct
    25021 cggcctcggt gtcgaccttg ccgaagacgg cgtcggggtg ctccgcggag gtcttctcga
    25081 agaccggccc gaactgacgg cacgggccac accaggaggc ccagaagtcg acgaagacga
    25141 tgtcgttgtc caggatggtc cgctcgaagg tgtcggcggt gagttcggtt gttgccatgg
    25201 ctgtgccaac tgcgcccgcc cggaagctat tccagactca acccgtccag ccacagacgg
    25261 cttcggtcat tcggggacga gttcccaggc gacccgaacg gtggcgccca ccgagtgctc
    25321 gccggcctcc accggcatcc ctcccgcgga ggccttcatc gacatcatcg cgtcgcgtcg
    25381 ccccgccatc ggctcgaaac cggggacgcc gcctccctcg accacggcga ggacccgccc
    25441 gaggcgggcg ccggcgaggg cggcatactg ctgcgctttg tcctgcgcgt catcgaaggc
    25501 ggcctcgcgc gcatgctctg caagcggccc gcgctcggcg aggtcgaggg tgatcccgtc
    25561 gatgaccagg gcgttgcccg cggcatcggc aacggcgtcg acgagatcgt tgagggacgg
    25621 cagcgaccgc acgagcaggg tgagctggtg gtatgccgtg tagcccgcca ccgcgctgcc
    25681 gtcgttgttc caccgcggct ggacggaggc gctggtcgag gcgatgtcgc ggtcggccag
    25741 gccatgggcc cgggcggccg cgctgacctg acggacggtg tcagccgcgg ccgagagcgc
    25801 agcggccacg ccttcggagt cgcagcggat gcccagggcg acgcggacga cgtcgggggt
    25861 ggcactggcg gtgccggtgc cggtcacttc gacgcgagcg ggtcggggac cacggtgttc
    25921 ggacggcata ctcatggctc accttacgac ggcagccggg cggccgcagc ggcgctggta
    25981 gactaggtgg gttcgccggg catgacccgg cgatttcctg gcgcaccgcg cgcctgaggc
    26041 acgtcttatc cggtcacctt ggcggtggct cgagacgcgt ctactcgagt caccggaagt
    26101 agctgatcca cgtcatgccc acccctaaac gttctggcgc gcccaagaag gcccgctgga
    26161 ccaccgccca gaagactgcc tccaagtccg gccccaagaa gccgcaccgc ggacaggccc
    26221 ccaccgagag caagccgccg cgcgccgagc gcgccccgag gcacgtcgcc cgcaccggtt
    26281 cgggcgacgc tcccacctcc cggtatgccg cccgctcccg cgacgagcgc cccgcacgtt
    26341 tcgagcgcga cgagcgcccg agccgtccgg cccggttcgt ccgcgacgat cgccccgagc
    26401 gtccgacccg cttcgaccgc accgatcgcc cggctcggtt tgagcgcgat gaccgtccgg
    26461 cccggttcga gcgggatgac cgctcggcac gcccggcccg ggtcgaccgg gatggcaacc
    26521 ccacacgtcc ggcgcgcttc gagcgtgatg accgcccgcg ccgtttcgac cgcgacgacc
    26581 gcccggcgcg cttcgagcgt gatgaccgcc cgcgccgttt cgaccgcgac gaccgcccgg
    26641 cgcgcttcga gcgggacgac cgcccacggc gtttcgaccg cgacgaccgc ccggcccgtc
    26701 cggcgcgtga tgaccgtccc gctcggttcg agagggacga ccgcccctcg cgtccggccc
    26761 gtgatgaccg tcccgctcgt gatgaccgtg cggctcggtt cgagcgggat gaccggccgc
    26821 gccgcttcga ccgggacgac cgcccggccc gtgacagccg tccggcccgt gatgaccgac
    26881 ccgcccgcga cgaccgggct cctcggttcg agcgggacaa ccgcctggct cgtccgggtc
    26941 gcttcgaccg cgatgaccgt ccggcccgtc cgggtcgctt cgaccgcgac ggtggaccct
    27001 cgcgtccgac ccggttcgag cgggatgacc ggccgcgccg cttcgaccgt gacgaccgac
    27061 ccgcgcgccg cgatggtgag cgggcggcat accgtgagcc gttcgtcgac ccggagagcg
    27121 agcgcctcga agccgactcc tggacctcgt cggcccgcac gccggtctcg accaacccgg
    27181 tgacggtggc cgaggacaac ggtttcgcct ccctcggcct gcccgagcgg ctcgtcgagc
    27241 ggctcgcccg cgacggcatc accacgccgt tcccgatcca ggacgcgacc atccccgacg
    27301 cgctcgccgg ccgcgacgtc cttggccgtg gccagaccgg ctccggaaag accctggcgt
    27361 tcggcctgcc catgctcgct cggctgctcg ccgacgggtc cggtcgggtc ccgcgtcggc
    27421 cgcgcgcgct ggtgctcgtg ccgacccgtg agctcgcgat gcaggtgtcc gacgcccttg
    27481 agccgctcgt gcacgtcgtc ggcatgcgcc acaagttggt cgccggcgga ctgtcctaca
    27541 cgacccagat cagcgcgctc gagcgtggca tcgacctgct catcgccacc cccggccgcc
    27601 tcatcgacct catggaccgc ggcgcggtcg agctcggctc ggtccaggtc gccgtcctcg
    27661 acgaggccga ccacatggcc gagatgggct tcctgcccga ggtgacgcgg atcctcgacg
    27721 aggttcccga cggtggccag cggctgctgt tctcggcgac cctcgacaac ggggtcgacc
    27781 aactcgtttc gcgctacctc actgacgccg tgacccactc gaccgacgat gccactgcct
    27841 cggtgacgac gatgcgccac ttcccgttcc tcattgaccc gcagcacaag aagccgctca
    27901 ccgctgagtt ggccaaccgt gagggtcgca cgatcgtgtt cgtgcgcacc aagctcggcg
    27961 ccgaccgggt cgctgctcag ctgcgcgagt gcggcgttgt cgcggcggct ctgcacggcg
    28021 ggctgtcgca gggtgtccgc aaccgcgtcc tcggcgcgtt ccgcctgggg accttgcggg
    28081 tgctcgtcgc caccgatgtc gccgctcgcg gcatccacgt cgacgacgtg tctctcgtcc
    28141 tgcaggtcga cccgccggcc gaccacaagg actacctgca ccgctccggg cgcacggctc
    28201 gcgcggggga ggagggcagc gtcgtcacgc tcgtcctgcc ccaccagcgc aagcagactg
    28261 agcggctgtt gcgcgaagcc ggtctcgagg aggtccccac gcgggtcagc cccggcgact
    28321 cgcttgtcgc ggccgccggt ggcagcgcac cgaccgggga gccggtcacc gatgaggcca
    28381 tgcgccggct gctcgagcct gagcggcgca gcccggcggg cgcgcgcggt ccgcggcacg
    28441 gcagccatgg cggcttccgt ccgcaccacg gtggcgcacc tcggcgcgag gaccgtcggt
    28501 tcgacactcg tgacgatcgt cggccgacat cccgtgacgc gcgtcggtcg gtgcgagact
    28561 aggggcgtgg gctcggagtc gaccgagccg tcgggtcagc cgacggcata cctcgcgggg
    28621 aagttgctcg tcgcaacccc gtcggtcgag ggtgacgtct ttcgccgatc ggtcgtcctc
    28681 atgctccacc acgacgccga cggcgcccag ggagtcgttc tcaaccagcc gctcgaagcg
    28741 ggcgtcgatg ccgtgctgcc cgggtggcag gccgtcacga cggcgccgca gacgctcttc
    28801 cggggcggtc cagtgggcac cgactcggcg atcggcatcg tcagcgttcc gggcgagggt
    28861 gaggcgacgc tgggggtcca acgcctcttc ggaggcatcg gggtcgtcga cctcgacgcg
    28921 cccccactcc tcgtggcccc ggaggttgcc gggttgcgga tcttcgccgg gtatgccggg
    28981 tggtccgaag ggcaacttga gggcgagatc gcgcgggggg actggtacgt cctggacgcc
    29041 gagccgcggg acgccttcac cgacgacccg gaaaccttgt gggccagcgt gttacgacgc
    29101 caacgcgaca gcctggccct cgtggcgttc tatcccgacg acccgtcgat gaactgactg
    29161 cgctgctcct cgcggatcgg atggaaacgc cgcaggtcga agcgtggaag cgcggggctg
    29221 accagcggcc gtgatcgtta ccctgtctgc catgacctac gccggtgaca tctctccgac
    29281 cgacaccttc gccgccctgc aggctgaccc gcaggccgtc ctcgtcgacg tccgcaccag
    29341 cgcggagtgg gcctatgtcg gcgtgcccga cctcgcgcgg gtcggcaggg acgccatcct
    29401 catcgagtgg gtgacctatc ccggtggcgc gcgcaacgac aacttcgtcg accaactgcg
    29461 cgaggccggg atcgccgaag gccagtccgt ctacttcctc tgccgctcgg gggtgcgctc
    29521 caagcacgcg gcggacgcgg cgaccgaggc cggtctcgga ccggcataca acatcgtgga
    29581 cgggttcgag gggcaactgg acgcgaccca ccaccggggt gtcggcgggt ggaagaccgc
    29641 tggcctgccg tggcggcagg ggtgagcgtc ggcgacgagg tgcgccggtg gcgacctgac
    29701 acgtatgccg tgcgcggtgg cctcacccgt agcgagttcg acgagacctc cgaagcggtc
    29761 ttcctgacgt cggggttcgt ctacgactcg gcacaacagg ccgaggcggc gttcaaggac
    29821 gagatcgaca agttcatcta cagccggtac ggcaacccga cggtcgcgat gttcgaggag
    29881 cggttgcggc tgatcgaggg tgccgaggca tgcttcggca cctcgtcggg catgtcggcc
    29941 gtcttcgtcg ccctggccgc cctctgcggt gcgggtgacc ggatcgtcgc ctcgcgcgcg
    30001 ttgttcggct cctgcttcgt catcatcgac gagatcctgc cgcggtgggg cgtcgagcgg
    30061 gtgctcgtcg acggggccga cctcgaccag tggcgggcag ccctcgaccg acctacggct
    30121 gcagtgtttt tcgagacacc cagcaacccc atgcaggaac tcgtcgacat ccgtgcggtg
    30181 tccgacctcg cgcatgcagt gggggccaag gtcgtcgtcg acaacgtctt cggcacgccg
    30241 gtctactcca agcccctgga gcatggcgcc gacatcgtcg tctactcggc gaccaagcac
    30301 atcgacggtc aaggccgggc actcggcggg gcgatcctcg gcccggccga ctacatcggt
    30361 ggcccggtga agaacctcat gcgccacacc ggtccggcgc tctcgccctt caacgcctgg
    30421 gtgatgctca agggtctgga gaccatgtcg ctgcgggtca accgacagca cgacaatgcg
    30481 ctcgcggtgg cggctttcct tgccgagcac ccgcgggtca cgcgggtcct gcacccctgg
    30541 cgcgaggacc acccgcagca cgacctggcg cgtcgacaga tgctcggtgg cgggaccgtc
    30601 atcaccttcg ccatcgacgg cggcaaggac gatgccttcg cggtcatgaa cgccctgcag
    30661 gtcgtcgaca tctcgaacaa cctcggcgac gccaagtcgc tcgtcaccca tccggcgacg
    30721 acgacgcatc ggcggctgac ccccgaggcg cgcgctgcgg tggggatcac cgatgggacg
    30781 atccggctct cggtgggtct ggaagacccc gccgacctca tcgacgacct ggctgcggcc
    30841 ctggacgccc tcggctgagc gccctcgggc tcagcgcacg aggtctcccg gacacacggt
    30901 gaccgggaac ggcaggtcgg ccgtgagcga gtcggcggcc gcggcatacc ccgtctgctc
    30961 gaaggtgccc ccgcgcagtg tccacgtcgt gatggacggg acttggggat cgagcaccca
    31021 gtaggcggcg gtccccgcct cggcgtagag gtcccgcttg agcgtcaggt ccaggcggcg
    31081 ggtgctcggt gagaggatct cgacggcgag cagcggagct cgcggcaggt ccttctcggt
    31141 gaagtcgccg ctgcgagcca cgatgaggtc ggggacgacg acggtgcgcg gcgagaggac
    31201 cacgtcgaag ggcgccgtca ggacttccaa accctcgggg caggcaccca acagcaggac
    31261 ggccagacgc aacgacatcc gctgatgccg atagccgggc gacggggtca tgacgatcga
    31321 cccgtcgaga agctcgtggc ggtgcccgtc gtcgggcatg gcgtcgagat cctcacgggt
    31381 gaactccccg gtccgggcca ggacggtcat gagacccatg gtgcctcctt acggtcatag
    31441 gcgcgagtct cctggatgtc gccgacaccc ctcgggcgat atccacaggg gcgcctggcg
    31501 tcgtggttcg gttacggttg tcggtatgcc gcgtgcctcc tcgatgccgc ccgacccgtt
    31561 cgagccccaa ccgatgccgt ccgagccggg caccggcacg cgcaccagcg tgctggagcg
    31621 cgaggagacc gtcgttgagc cccaggtgca ggagccgggg gaccacgagc ggttcagcca
    31681 ctacgcccac aaggacaaga tcatggaagc catggtcatg ggcacgccct gcgtcgcgct
    31741 gtgcggcaag gtctggatcc ccagccgcga ccccaagaag ttcccggtgt gcccgacgtg
    31801 caaggcgatc ttcgaggacg acagcgccgg ctgggtcccc gaggacgaat agcccacgtc
    31861 gccccctcgc cgcggccttc tcctgcttag agtgggagag gtcggaaggg gacgacatgt
    31921 cgatcatcga gggcaccaag caggccgcga cggatcgcct gcgcgtcatc gccgggccgg
    31981 cctaccggcg ggtcaagcga gagatccagt ggcgtcggtg cggcgccgac ctggccttgg
    32041 agcgggggcc cgtctcaggg ttcccctggg tcgtgatggc gcacgagacc ccgctcatcc
    32101 ggcccctgtc cgggcttgag caccgactgc aggtcaacaa ggtgaccaac ctgcgcctgg
    32161 ccatcgaacc cctcgatggg cgagtgctgc agccgggcca gcggctctcg gtctggtggt
    32221 ttgtgcgggc gccgacggcc cgccgaggct tcctcgaagg actggtgctc agccagggac
    32281 gactcaccgc aggggtcggc ggcggcctgt gccaactcac caacctcatc cattggatga
    32341 caattcacac cccgctgacg gtggtggagc gatggcggca tacctatgac gtcttcccgg
    32401 acgccgggcg gacccagccg ttcgccagcg gcgcgacctg ctcatggccg tcgcttgacc
    32461 tgcagatcga gaaccggacg gacacggcat accggctggg gttgcggctg accgacacgc
    32521 acctggtcgg ggagtggcgg gcagcggtgg aacccaccag aagctacgag gtctacgagg
    32581 cggcccacga gatcgcgacc gaactgcccg ggcggttcgt ccggcgaaac ctgctgcggc
    32641 gcaaggtgtt cgacatggtg ggggagcaga tcgacgacga gttcgtcgcc gagaaccatg
    32701 cgctgatgat gtatccaccg ttcctcagcg accggccgac cacgccaccg gggccatcgc
    32761 ccgaatgacc ggcccccggc tcacccgagt ggggcgaact ccgcggtgaa gtgccgcaac
    32821 tgcggcggtt cgacaacgat ctggtagccg ggcaacgagt ccttgaccgc catgacccgc
    32881 tcggccactt cgatgacgta gtccatgtga ctctgggtgt aggtgcgccg ggggatcgcc
    32941 aagcggacca actccatcgc cgccggcgac tcggagccgt cggggtggcg accgaacatc
    33001 gccgacccga tctcgcaggt ccggatgcca ccgtgttcat agagcgcgac ggccagcgcc
    33061 tggccgggga actgcagcgg cgggatgtgg gggagcaggg ccttggcgtc gatgtagacc
    33121 gcatgcccgc ccgcgggctt gatgcacggc atacccaatg cctccagggc atccgcgacg
    33181 taagcggtcg agcggatccg gtagcgcagg aagtcgtgcg agacgacctc cttgagcccc
    33241 tgcgcgagcg cctccaggtc gcggccggcc agaccgccgt aggtcgggaa gccttcggtg
    33301 aggatgagca ggttgcggca cttgccggcg atttcgtcgt cgttgagcgc gagccaaccg
    33361 ccgatgttgc ccatcgggtc cttcttggcc gacatcgtca tgccgtcggc gcaggccgcg
    33421 atgtcgcgga tgatgtcgcg cacgtcgcgc tcaccctgac cgggctcgcg ctcgtggatg
    33481 aaccaggcgt tctcggcgaa cctggccgcg tcgaggaaga gcggcttgcc gtgtcgatgg
    33541 gcgatctcgg cggcggcccg caggttggcc agcgagaccg gttggcctcc gccggagttg
    33601 ttggtgatcg tcatcatcac gcaggggatg tccgaaccgc gttcgcgcag aatggtttcc
    33661 agggcgtcga gatcgatgtt gcccttgaag ggatgcagcg ctgcggggtc gcggccctcg
    33721 gcgatcacca ggtcgacggc ttcggcgccg gtgtattcga tgttggcgcg ggtcgtgtcg
    33781 aagtgggtgt tcgacgggat gaccttgccg ggcccggcca gcgcctggaa gaggatccgc
    33841 tcggcggcgc gcccctggtg cgtggggatg acgtgcgcgt aggggaacag ttcctggacc
    33901 gcgtcacgga agatgaagta ggacggcgag ccggcgtagg actcgtcgcc gtgctggatc
    33961 gcggcccact ggtcgcggct catcgccccg gtccccgagt cggtcaacag gtcgatgagg
    34021 acgtcatcgg catgcagttg gaacaggttg tatgccgcct cccgcacctt ccctcggcga
    34081 tagtcggcgg tggtcatccg caatggctcc accgagtgga tgcggaaggg ctcgaacacg
    34141 gtccggaacg gggcttcagt cacgaacgag actgtatgcc gccgaaatcg tccttcgtca
    34201 gtcccgacgc cgaagtttct ttcgccaggc tcggttcagc gcacgaacac gcgccaaccc
    34261 caggccgcca gatcgatcct catcccctcg cggccccgca ggggtgagcc gtcgatggcg
    34321 tcgacccagg ggccgtcctg ggggccccga cccagcgtca cgctgcggtg cactcccgtg
    34381 aagttgaaca ccgcgaaaac gctgtcctcg ccctgggtgc gcacgaaggt cagcaccgag
    34441 gtctccatgt cgttggggac cttgaccatg cgaccacctg cggccccgtt ccacagcgcc
    34501 gggtgagcct tcttgagggc aaacagcgtg cggtacaact cgccctgggg gtcctcccgc
    34561 cagatgatct cgtccttgtc gaagaaggcc aggcgcttgt cgaagccggc ctcctgaccg
    34621 ttgtagatga gcggcatacc ttcgccgatg accgacaaca caatggcgga ctcgcgcatc
    34681 ggcccgaact gctcgaactc ggtgccctcc caggagttct tgtcgtggtt ggagacgaac
    34741 atcatccgga tggcgtcgcg cggccaggac gactcgttcc aggcgtagaa gatgtgcagg
    34801 gcggtgacgt ccgatcggcc cacggcgatg tgatgcatcg tttcgttcca ggaccagccg
    34861 taggtcatgt cgaaggcggc ctcgtggagg tctcgggctt cccattcggc gagcatgaag
    34921 acgggcttga tcgcgtcgag ctcggcgcgt gcctgctccc agaagtcgag cgggacgaaa
    34981 ccggctacgt cgcagcggaa tccgtcgacg tcggcttccc gcacccagta gcacatcgac
    35041 tcggtcatgt agcggcgcag ccccgcatgt gagtagtcga gatcgatgac gtcgtcccag
    35101 tcccaccacg gagtcggccg gaagtcgccc ttccagtccc gcgcgtacca ctcggggtgc
    35161 tccgcgacca gcacgttgtc ccatgacgtg tggttggcca cccagtcgag gatgaccttc
    35221 atccccaagg cgtgcgctgc cgcgacgaag gagcgcagat cgcgcagcga gccgaactcg
    35281 gggttgaccg cgcggtagtc gcgcacggca tacggactgc ccaggccccc cttgcgccgt
    35341 tcctcgccga tggggttgac cggcatcagc cagatgatgt ccacgcccaa ccggcgcagc
    35401 cgggggaggt gttcgcgagc cgcctggaag gtgccctcgt gggtgaactg ccgttggttg
    35461 agttggtaga tggtggccgc gcgagcccac tgcggatgcg cgatccgcgt ctgcggccgc
    35521 ggggcatacg tggacttttc ggcgtggtcg gtcacgggcc ctaagcgtat gccgctcgcg
    35581 acgggtcccg cctacccgct cacggccgcc cggctggggc cctccgccac caggccctga
    35641 cggacccggg acacgatgcg gctcatcgcc accggggtca cgccgatgta gcgcgcgagg
    35701 tccttctgcg gcacgaggct gaccaattcg ggccgctcct ggacaaggag ccggtagcgc
    35761 tcctccgcgt tgagggtgag caactcgcgc tcgcgcctct cctgaagggc ggtcgaggcg
    35821 atcgaggcga tgagggccaa ctgttgccat tcgcgatagc ggttcgacag cgcccagaag
    35881 gtcgagatgt cgacgtacct cgcacggacc cgggtgatgc cgcgggcgct gtagtgcatg
    35941 tacgccgacg gaccgaagtc cgcactgtcg gggtccaggc cgacgaaggc cgccgccgcg
    36001 cgggggaggg ggtgggccaa cgcgggcacc gaggagagca actcgcccgg cccgacgaac
    36061 cgacggatcc agtctcggcc cgggcgatcg gaggtcgcca ggctgacgat gccgtcgacg
    36121 atgaagaaga agcgtcggtc cgtcgtgcct gcgtcgaaga gcaactcacg gggctcgatc
    36181 accgcccagg acatcgcccg ctggaagtgg tggaagtccg gcagcggggc ccccgcgact
    36241 cgctccaggt gagcctggaa ggcttccgga ctatgcgagg tgggcaccgc gcgactctcc
    36301 tttgatgact gtctgaacgg aacccgagcc gcgcagagca tggccaagtg gccggtacac
    36361 gtgtcccctt ggatcccgct gacgaatccc cggggggagt ctacgacggg cgctgcctgt
    36421 ttgggaggaa agggaccaac aagatcctcg gatactcgcg agttctcggt gggtcatggt
    36481 ccgtgtggac gggacgagcg gctcggcgtg cgaaggtagg ggcatgacgc agccttccgg
    36541 gtggtacgac gacccgtcgg atcacagccg tttgcgctat tgggacggca tcctctggac
    36601 ggatcggacc gtgccgaagc aatctccgac ggccgccgac tcgacgatcg ggcgggcggt
    36661 cgacccgtat gccgcgtcga gccaccacgc acccaccggt cccggtcccg gcccctactc
    36721 accggcggct ccgacctacg ggcagccacc aactccttac ggccaggctc cgactccgta
    36781 cggccagccg ccggcgggtc gcgactacgg agcagcgccg acgtatcaat ggcgcccgtc
    36841 cggtccgacg acccccgacg gggtgcccct ggcccagtgg tggcagcgac tggtggccgc
    36901 cgtgatcgac aacatcatcg tgcagctcgc gagtctcgtg ctggcgttcc cctggtggcg
    36961 tgacttcctg gtgtggtacc tcgacttcct gcagacggcg accgtgtcat cagggtcggt
    37021 cgacccgctg acactattcg gcgagatcaa caccgaaatg gctcgctata tcgtcccgat
    37081 cgtcctgatc cagtcagcgg tgacgttcgt ctatcacgtg ctcttcctcc accggaacgg
    37141 cgccagcgtc ggcaagctgg ctctgggtat ccgggtccga cggttcgatc gacctgggcc
    37201 gctcaccctg ctcgaagcgt cccgtcgtca ggccctgcag ttggtgtttg gcctggtcgg
    37261 cctggttccg ttcatcggca tggtgacggg cgttgttggc tacgtcgacg tggcctggct
    37321 gctgtgggat cagcgtcgcc agtgcctgca cgacaagatt gccgacaccc tcgtggagca
    37381 gaagccgccc acccggcggg tgtgacggcc ggtcgcccag gcacggttag ttcgtcgcga
    37441 ccggacccgg cgcggggagg ctgtgccgtc cctcgtgcgt gcggaagatg agcgacgtct
    37501 ggacgtggac gacgccagga tgccctgaca ggtggtcaag gaccacgccg cgcagggtgt
    37561 ctggtgactg cgcgacgaga tggacgagga agtcgtcggc gccgctgacg ttgtatgccg
    37621 ccagcacgcc aggtagccgc gacacatgct cgccgaactc gtcgacccgg cgacggtcgg
    37681 tgccggccag gcgcacggcg accatcgctt cgatggggta gccgagtttc ccgcggtcca
    37741 cccgagcggt gaagccacgc aggatgcccc gcgccagcag tgaccggacg cgaccgatgc
    37801 aggtcgactc cgccagcccg acggccctcg cgagttggac attggggacc cgggcatcgg
    37861 cgacgagggc atccacgatc gctcggtcga catcgtcgac gggcgccggc gtgcggggcg
    37921 ccggccctgg aggcaacgcg cgtgggttgt tcggcacagg gccactgtaa gcgtccgtgg
    37981 ccgaggaatc tgcggcatca cggcctaaga aggcgaggat ccacgggtct atggagcttg
    38041 atccgtggat tgtcgacact ggaggtatgc gcgcagacac ctccgccgtc cacgccggtc
    38101 gcgaagacct ggtcgccctc ggcgtgcacg tcccgcctat cgacctgtcg agcacctacc
    38161 cgatcggcga cctcgacgcc ggcggcgcgg catacgagac gctcgccatg ggtggacgcg
    38221 gtgaagccgc ggcgaccctc gtctatcagc ggctctggaa ccccaacgtc gaccggttcg
    38281 agcgggcctt ggcgcagttg gaaggcaccg aggaggccgt cgccttcgcc tccgggatgg
    38341 cagcgctgac cgccaccctc atcgcctgcg tcgccgccgg cacgccgcac atcgtcgcgg
    38401 tgcgcccgct ctacggcggg tcagatcacg tcctggccac tggcctgctc ggcacccagg
    38461 tcacctgggc cacggccgcg accgtcggtg aggcgatcac cgatcggacc gggttggtgc
    38521 tgttggagac ccctgccaac ccgaccctcg acctggtgga tatcgctgcc gtcgtcgccc
    38581 aagccggtcg tgtgccggtc atggtcgaca acaccttcgc aacgcccgtc ctgcagcaac
    38641 cgcgtcgcca gcgcgcgacc ctcgtgctgc actcggcgac gaagttcatc ggggggcacg
    38701 gtgacctgct cggtggcgtc atcgcggcca gccccgaatg ggccgtgcgc cttcggcaag
    38761 tgcgcgcgtt gaccggcgcg ctgctgtatc cgctcgcggc atacgacctg caccgcggcc
    38821 tgcagaccct ccccgtgcgg gtgcgcgccc aacaggacaa cgccatcgcc ctcgctggct
    38881 ggctcgcagg tcacccggcc gtcgagcgcg tccactaccc gggtatgccg ggtgcggacc
    38941 ccgagggcct gctgggtcgg cagttggccg gccccggagc cgtgttggcg atcgcgctgc
    39001 gcggtggctt cgacgcggct gccgacctcg tcacgcggac tcggctggtg acccacgccg
    39061 tgagcctcgg cggagtcgac agcctgctgc agcaccccgc cagcctcacc catcgcccgg
    39121 tcgccgccga ggccaagccg caccccaacg tcgtgcggat ctcggtcggc ttggaggacg
    39181 tccgtgacgt catcgccgac ttcgaccagg ccctgcgcgg ggtcggggag cgtcggttgg
    39241 tcgacgttct cagcggggcg gacgcactcg cccgctgacg tcaccgaggt cgatccgggt
    39301 gccgtccggc cccggtgccc aggcggtgat ggcgacctcg tcaccgtcct gcaggtagct
    39361 gcgcgacgac ccgtcggcca actcgaaagg ctcctggcca ccccaggaca gctcaaggaa
    39421 ggacccgcgc tgccccttct cggggccgct gatcgtgccc gagccgaaca ggtcgccgtc
    39481 gcccacgctg gcaccgttga cggtcatgtg ggcgagcatc tgggcggggg accaatacat
    39541 gtcgcggtat tccggccggc tgaccacctc gccgttgagg cgcacctcga ggtgcacgtc
    39601 caacccgaag gcggcgccac ccgagccctg cacgccggtg ccggtgccgc gcaggtaggg
    39661 caggacctcg ggatcggcct cgtcccgcgg caggtcgacc cgggcggact gcagggccgc
    39721 catcggggtc acccacgccg agatcgacgt gcagaagctc ttgccgaggt aggggccgag
    39781 cgggacgtac tcccacgcct ggatgtcgcg ggccgaccag tcgttgagca ggacgacccc
    39841 gaagaggtgc tcgccggctt cgtcgacgct gatccgctcg cccatgctgg tcgccccgcc
    39901 gacgacgtaa ccgagctcgg cctcgatgtc gagccggacg cttgggccat agacgggcac
    39961 cgggtcctga ggccccttgc gttggccgca gggacggtgg aagggggtgc cggacacgac
    40021 caccgtgccg gagcggccgt ggtagccgat gggcaggtgc ttccagttga ccggaagggc
    40081 cggtgagtcg ggtcggaaga tcatgccgac gttgcgcgcg tggtgggcgc tggcgtagta
    40141 gtcgacgtag tcaccgacgg tgatcggcag gtgcagggtc accgcgtcca gcgggagcag
    40201 gtgtggctgc acagcgtgct ggtaggccgc atcgttgagc acctgagtca gccaggcgcg
    40261 ccccgcggcc cacgccgtcg tgccctgccc gaggaacgcg ttgagcgagg gcgtcgccca
    40321 cgcggctccc gcggcatacc ccgtcgcggc cgcggcgcgc gcggcgtcca ggacgaggtc
    40381 gccgatgcgg accccgacgc gcggggctgg gtcagcgacc gtggagaaga ctccgtaggg
    40441 gaggtgggca agcccgaagg ggtggtcggc ggggaggtcc agccaggtca ttggtgctcc
    40501 ttgtcgatga gacgcaacgc cgcaagctcg ccgagcgggt ccgcgacggt gcagcatccg
    40561 tatgccgtga acgcgcaccg cacgcgactg gccgcgtcgg tgccgagtgc ggtgagcagg
    40621 tcgacaagcg gggcagcgtc gcgctcgccc agcaactcgg ctacctcggg ttcggggacg
    40681 ccctcggcgg tcgcgtgcac cgctgcgagc acgttgagca ggccgtgctg ggggccgcca
    40741 tcggggcccg ggtggtcggc tcgcagcacg tggtgcaggc cgccggtgag cttgaacggc
    40801 aggtcgcgct cacggcaggc ggcaaagaag cgggccaact cgacctcgtc gggccaggcc
    40861 cagacctcgg tggcacccgt gcggaacttc gcctggacca acggttcgcg accggtccgc
    40921 agggcaacag cggcgacggc gtcgatcgcg gcggtgaacc cgtcgcgagg gatctcgacc
    40981 gtgagcgcta ccccgaggtc cagcaggctc tcccactcgg ccccggctcc gacctcgacg
    41041 ccggcgacct cgacggtgct gccggcaagg gcggcggctg cggcgcggac cggctcgacc
    41101 ggcataccgg ggcgggcgat caggccgacg cgaaggcgcg caccgccagc gagcgcgcgg
    41161 acctccgcag ccgctgtcgc ggggacgagc aacggcccca cgaactcgcg gtatgccgtg
    41221 cgcgccagat gctcagcgac ggcgcgcggg aggggagcca ggccgggcgg gaagaccgcg
    41281 gcgtcgtcga ccagccgctc gatcagtgct cgcggcagcc gggatcccat gtttggcagg
    41341 ctagcccacc aggcctggcc gacagcggcc cgcgggtcgc tgccaaccac gggaaacctg
    41401 cgcaggttgc gcacccccga gtccgccggc tcggggagat agcgtcacct gtcgtgaaga
    41461 actccaccgg catgttcggc cccgacagcc cccgcggtgc acgcctgggc tggtcgggcg
    41521 tgatcggcgc cgtgaccttt gccgtcgcgt cggcgcggct gggtcccgcg gtcgccgccg
    41581 tcctcggctg gatcctcggt gctggagcgt tcgtcctctg gaccctcgtg gtcgttgggc
    41641 gcctggatgg gccgggcgcc gagtcccacg ccacccgtga ggactcgacc cgtgtcgtca
    41701 gtgacctcat cctcatcggg ggaagcatcg ccagtctcgg cggtgtcggc gtcctgctcg
    41761 ccacgcaccg cggggagggc ggggcgccct ggcaggcggt gctcggggtg ctgtgcgtcg
    41821 cggtgtcctg ggtgctcgtc cacacggtct acctgctgag gtatgccgcg ctgtactacg
    41881 cccagacgcc tcgagggatc gacttcaacc agaccgagcc gccggactat cacgacttcg
    41941 cctacctcgc cttcaccctc ggcatgacgt atcaggtgtc cgataccgcg atcagcagct
    42001 ccgtcttccg gcggaccgtg ctgcgccaca ccctgctgtc ctacgtcttc ggctcggtga
    42061 tcctcgccac caccatcaac ctcgtcgtcc aattggccag tccgacaggg tgattcgagg
    42121 cgtctgtggg gcgccaaaat ggggcattcc cggcgtcccc ccgtgcgcct acactgtcac
    42181 ccgtgtcaac ggcaccagtc aaagaacccg aggtgatctc gcttgaggcc tcggagttgg
    42241 cgcttccctg gatcacgctg gtctggaacg acccggtcaa cctcatgtcg tacgtggtct
    42301 acgtcttcga gacctatttc ggctacccgc gggccaaggc cgagaggctg atgatggacg
    42361 tgcacacctt ggggaaggcg gtcgtctccc tgggcacgcg cgaggcgatg gagaccgact
    42421 gcatggccat gcagggcttc ggcctctggg cgaccttcga aaagaaccag taatggcccg
    42481 cgcgtttcgg cgccgcggcg cccgcatggt cgcccggctg gatcgcaacg agcgcgaggt
    42541 cattgcgagc ctcatggagc agacccggtc gctgctggct cccgacgttc cctcgactgg
    42601 agacgccttc accgacctga tggcatcctt ggagtcgggg gcgaccgccc cggagggcct
    42661 gcctcggggg accgacccgg gcgatcgaga ccccgcgctg gccaggttat tgcccgatgc
    42721 acatcggggg gaccctgcgg tggcagcgga gtttcgccgg ctcaccgagc aggaccttcg
    42781 gcggcgcaag agcgacgcgc tgacagcatc gatcgacgcc ttgcgtgccg aggcggatcg
    42841 cgaccggctg agcctggagc ccgcgaccgc acagtcgttc ctcatcgctc tcaccgacac
    42901 cagactgctc atgggcgagc gactggggct gcgcaccgag gaagacgtcg acgcgtggga
    42961 ggcacgggtc gaggccgacc cggatgatcc gttggcttac gccgcagccc tctacgactt
    43021 cctcacctgg cttcaggacg gactcgtcgg agccctcatg ggccgccggg ccttccccga
    43081 tgcgtgaccg acaagtgacc gacgcatgaa gggaacgtga cggaggcctg aacgtggacg
    43141 gcaccgaggt ggacgacgat gtgaccgagg tgaccgaccc acgattgtgg gacgagcggc
    43201 atacctcctt atcctgagtg ccgtgggcga tgcgccaatc gggatcttcg actcgggtta
    43261 cggcggattg accgttgccc gagcggtcct ggaccaactg ccgcacgagt ccatcgtcta
    43321 cttcggtgac acggcccgca cgccctacgg cccgcggccg atcgggcagg tgcgcgagtt
    43381 cgcgttggag tgcctcgacc accttgttga ccgtggggtc aaggccttgg tcatcgcgtg
    43441 caacaccgcc agcgccgcga tgctgcacga tgctcgcgag cgctatgacg tgccggtcat
    43501 cgaggtgatc cggccggcgg tccgccgggc ggccgcagcg actcgcacca accgggtggg
    43561 tgtcatctcg accatgggaa cccatgccag ccgtgcctac cccgacactt tcgcggccgc
    43621 gccacacatc gaggtgacgt cgcagccgtg cccgcggttc gtcgagttcg ttgagaatgg
    43681 cgtgaccggt ggcgatgaat tgttagccgt cgcaagggaa tacctagcgc cgttgcagga
    43741 agccgaggtc gacaccctca tcctcgggtg cacccactac cccctgctca ccggcgtgat
    43801 ctcctacgtc atgggcgatc tggtcacgct cgtttcgtcg gccgaggaga cggccaagga
    43861 tgtttaccga gtgctggccg acggcggcgc gctgcgcccc gacgggttgc cagcgccgcg
    43921 acacgagttc gtcacgacgg gtgatccgca gaccttcagc cgactggccc ggcggttcct
    43981 tggaccggag gcggcgatcg agtcgcgccg ggaggcggtc gcgccaggat gagcgcagag
    44041 acctcaccac ggatgcgcgt cacgatcgtc gggtgctcgg ggtccttccc cgggccgcag
    44101 tcgccggcct cgtgctacct cgtccaggcc gagcacgaag gccggacatg gagcatggtg
    44161 ctcgacctgg gcagtggggc cctcgggccg ttgcagcgtt acctcgaccc cacgaccctg
    44221 gatgccgtcg tcctcagcca cctgcacgcc gaccacttcc tggacctgtg tgggctctat
    44281 gtcatgcagc gctaccaccc gcgaggcttc cgcggacccg tgccggtgtg gggtccggcc
    44341 ggtacgccac aacgggtcgg gctcgcttac tacgggcatg acggcaccga ggtgtcggag
    44401 ttctccttca cccgcgtcgt cgaccgagcg accttccgca tcggcccgtt ctcggtcacc
    44461 gcgcgtcggg tcaaccaccc cgtcgaggcc tacggctatc gggtcgagtg cgcaggccag
    44521 gtgctggtgt tcagtggcga caccgacatg tgcgacgcgc tgatcgacct cgccgccgat
    44581 gccgacctgc tccttgccga taccgccttc gttgacggcc gcgatgatga gcgcaacatc
    44641 cacatgaccg gctcacgagt cgcgcaggtc gccctcgccg ccggaggcgt caagcggatc
    44701 atgctcacgc acctgccgtc ctggaacgac ccggaggtat gccgcgccca ggccgccgcg
    44761 gtgtggcccg gacaggtcga ggtcgctgcc cccggcctgg tcatcgacct ctgacggtcg
    44821 acgcgcccac tcgatcaccg gtcgggacgc gcggcggggg agcacggcat acctgcgtag
    44881 tctgtccgcc gtgagcaacg caacccgcca tgacggccgc gcccaggacc agacccgcga
    44941 ggtgcgcatc acgcgcaact ggctcgacca cgccgagggg tcggtgctcg tcgagttcgg
    45001 acggacccgc gtgctgtgcg cggcgtcgtt caccgaaggg gttccgcgct ggctcaaggg
    45061 gcagggcaag ggctgggtga cggcggagta cgccatgctg ccgcgctcga ccaacacccg
    45121 ttcggaccgg gagtcggtca agggcaagat cggtggccgc acccacgaga tcagccggct
    45181 cgtcggccgc tcgctgcgcg ccgtcatcga catgaaggcc ttgggcgaga acacgatcgt
    45241 gctcgactgc gacgtcttgc aggccgacgg aggcacccgc actgccgcga tcaccggggc
    45301 ctgggtggcc ttggccgacg cggtcgccga tgccaaggct cgggggctca tcaagcctga
    45361 cgccgcgccg ctcaccggat cggtggccgc ggtgtccgtc ggtgtcgtcg gcggcatccc
    45421 ggtgatcgac ctggactacc ccgaggactc cacggccgag accgacatga acgtcgtcat
    45481 gaccggcgac gggcgcttcg tcgaggtcca ggggaccgcc gagggggagc ccttcgaccg
    45541 ggcgttgctg gggcaactgc tcgacctcgc gaccaagggc tgtgccgacc tcacggccgc
    45601 gcaacacgcg gcgctcgccg ccgacccgcg ctcgcgcgcc tgaacccgac caggggtatg
    45661 ccgtgacccg ccgcctcgtc ctggccaccc gcaacgccca caaggttggt gaactgcgcg
    45721 agatcctcgc cgacgtgctc gccgacctcg acctgcagat cgtcgggctc gccgatttcc
    45781 cggaagttgc cgacgtcgtc gaggacggcg tgaccttcgc cgagaacgcc ctgctcaagg
    45841 cccgcgctgt cgctgcggcc accggcctgc ccgcgctcgc cgacgactcc ggtctggccg
    45901 tcgacgtcct cgggggtgct ccgggcattt tctcggcgcg gtggtcggga cggcacggcg
    45961 acgatctggc caacctggag ttgctgctgg cccaggtgtc cgacgtccgg gacgagcacc
    46021 gcggcgcggg cttcgtctgt gcggcggcga tcgcgctgcc ggacggccgg gcgcacgtcg
    46081 aggaaggtgt tttccggggg acgttgaccc gagaaccccg cggcagcaat ggcttcggct
    46141 atgacccgat cctgctcgtc gagggtgacg tgcggacgtc ggccgagctt tcgcccgctg
    46201 agaagaacgc gatgtcacac cgcggtaagg cttttcgggc gttggcggag catctgggag
    46261 ggatgctgga ggcgtgacct ccgaacagcc cggccaaagc gggcctcagg gcgtgctgcc
    46321 agtgcccgcc tgggacgccc ccgacccgtc cgagccgccc ggcgacccgt tcggtccggg
    46381 tgggtgggcg gtcggcaacc tggggtatgc cgtcgacggc ctggtcgccg gagcgaccgg
    46441 gctgctcgtc gctgaggcgc tcgatgtcgc gttcaccctc ggggggtggg tgagcggaaa
    46501 ctcctcggga cggtccccga tcctggccgt gggcgccgcg ttcgtcgacc tcacgcccgc
    46561 gtggctcaag gagtgggccg tcgccagttt cggcactggc gacaaggccg tcctgttcgc
    46621 cggcatggcg atcgtcctgg ccgtcctctt cgtggccatc gggctggtcg cgcgcacgcg
    46681 gcataccctc gcgaccgccc tcgtcggggt gctgggcgtg ctggccctgg tcgcccaact
    46741 cacgcgcgcc ggagcctcgc ccaccgaccc gatcccgctg gtcggcggct tggccgtgac
    46801 cctctggatg ttgccgcggt tggttgcccg caccgcgatt gaggggccca gccgcagaga
    46861 ggccctcggc ctggccggcg ccgcagccgg tgtcgcgctg gcgaccgcgg tgcgcgggtc
    46921 cggcggtgtc atcgccgcca acgcgagcgc gtcacgagcg gccgccgtcc ccgtgccctc
    46981 cggtctgccg attccggcgg ctgcgggggc cgagatcggg gtcggtgggg tcggcccgtg
    47041 ggcggtgccg aacgccgact tctaccggat cgacaccgcg ttgaccgtcc cgcggttgac
    47101 ggccgaagcc tggtcgttgc gggtctgggg tgaggttgag cgtgaggtga cgctgacctg
    47161 ggcgcaactg gtgggcaagc cgctcatcca gcgctacgtc accctcgcgt gcgtctcgaa
    47221 cgaggtcggt ggccatctcg tcggcaatgc cctctggacc gggtggccgg tgcgcgaact
    47281 gctgcgcgag gccggcgcca aggcgggcgc cgacatggtc ctctcgcgca gtatcgacgg
    47341 ctggaccgcc gggacaccgc tgtcggcgct caccgatgac cgcgacgctc tgctcgccgt
    47401 cgcgatgaat ggagtggcgc tgccgttcga gcacggcttc cccgtgcgtc tggtcgtccc
    47461 gggcctctat ggctacgtgt cggccaccaa gtgggtgacc gacctcaaag tcacccggtt
    47521 cgccgacgac atgggctact ggacgccgcg aggctggtcg gccctcgggc cagtcaagac
    47581 cgcctcgcgc atcgacgtcc cgcgctcggg ggctcgcctg cctgccggtc gggtcgcggt
    47641 ggctggcgtc gcgtgggcca tgcatcgcgg catccaggcg gtgcaggtgc aggtcgacgg
    47701 cggcccgtgg gccgacgcgc gactggccac cgaaccgacc gtcgactcat ggcggcagtg
    47761 ggtgttcgag tgggacgccg ggcccgggtc gcatgtcctc acggtccgag cccgcgacgg
    47821 ggcgggggag tggcagaccg ccgtgcagac cacatccgac ccggacggag cgaccggcca
    47881 ccactcggtg acggtggcgg tggcgggctg agacgagcgc gcgcttggct agagttggcg
    47941 ccgcgcgcgc gtggcggaat tggcagacgc gccagattta ggttctggtg ccttcgggtg
    48001 tgcgggttcg agtcccgccg cgcgcactct ctctcggnnn nnnnnnnnnn nnnnnnnnnn
    48061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48121 nnnnnnnnnn nnnnnnnggc aggtcgtgcg tcaccatgag gacggtcacg tcgagcccga
    48181 ggatgatctc ggccaactcg cggcggctgg cggggtcgag gttggaggac ggctcgtcga
    48241 ggacgaggat ctccgggcgc atcgccagga ccgtcgccac cgcgacgcgg cggcgttgcc
    48301 cgaaggacag gtggtgtggc ggccgatcgg cgtatgccgc catccccacc gccgcgagcg
    48361 cttcagcgac acgagcctcc aactcggctc cgcgcagccc gaggttggcc ggaccgaacg
    48421 ccacgtcgtc gcggacggtc ggcatgaaca actggtcgtc ggggtcttgg aagacgatcc
    48481 cgacgcggcg cctgatctcc gggagcgtgt ccttgccgac cggcataccg gacacctcga
    48541 cggtgccgcc cccggagccc gcgtcgcccc cgagaatgcc gttgaggtgc aggacgaggg
    48601 tggttttgcc cgcgccgttg gggccaagca gcgccacccg ctcgccacga tggacgtgca
    48661 ggtccgcccc gtcgagggcg cgggtgccgt ccgggtagac gtagtagagg ccctggacgt
    48721 cgagcacggg ggtcgtcatc gggtcagcac cgccgcgccc atcccgactg cggccatcgt
    48781 cggcagagcg gccgccacca ccaggtctcc cacgcgcagc ggggccgcgt cgaggaccgg
    48841 aagcgagccg gcatacccgc gcgagagcat cgccaggtgg acccgctcgc cccgctcgta
    48901 ggaccgcacg aagagcgctc cgaggcaggt ggcgagcacc ggccactgcc gcggcgagcg
    48961 ggcatcgaac ccgcgggagg ccagggcgat cgacatccgc cgcaggtcgc cggtgacgac
    49021 ctcgacgtaa cgaaccatga agcccatgat ctcgacgagt tgacggggca ggcgcagccg
    49081 ggtgaggccg gtgagcaggt cgcgtggctc ggtggtcgct gcgagcagga gggcggcgag
    49141 cacgccgagg gttcccttcg ccaacagcgc ccacgccgcg accaggcccg gttccgagac
    49201 cgagagaccc agcacgctga tgcgtggccc cgtcgcgacg aacggcagga ccacggcgaa
    49261 cacgacgaag ggcacctcca cgacgagccg cgtggcgagg tatgccgcgg gcacccgcgc
    49321 cgtccgagcg accagcatga gggcggcgag gtggacggca tagggccagg tggcctcgcg
    49381 gggtgtggcg acgacgagga ggacgaaggc gagggtcgag acgatcttga ggtgcgccgg
    49441 caaccggtga acggtcgact gaccgggcag gtgcaggccg tgcgcgtgct cagcgcccac
    49501 gcggggacct tgccgaacga cggcgcacga ccgcggtgag cccgagcccg acgatgagga
    49561 ccagggcgac tcccaccacc ccaccgaggc cgtcatagga caagaggccg gaccgttcga
    49621 cggcctggcc ggagatgccc tgatctgcgg ccacccgctc gagcccgtcc ggagcggtcg
    49681 acgcgaagcg gctcaagacc ccggccacca gcagcgtcac cagcacgccg agcgcccacc
    49741 accggcgacc gtgaccgctc atgcccgctg tccggacgac ggagccgggg cggcagcctg
    49801 gatcgacgcg gctcggatgc ggcgggcgcc gtgcacgagg tcggggcgga cggcgaccac
    49861 gctgccgacg accagcgcgg tgaggactcc ttcgccgaca ccgatgaggg tgtggacact
    49921 ggccatcgct gccaccaacg agccggcgtc caggggcgcc gtcccgccga ccatgaacag
    49981 tccggcgaag gtgagggcgg ccatcggcac cgagacgaag ccaccgatcc cggcggccaa
    50041 tggcaccgag ccgatccgcc gggggaggac ggccagcgcg aggcggaaca cggcataccc
    50101 ggtccacacg ccgaccacgc ccatgagggt gatgttcgtc ccgagcgccg tgagcccgcc
    50161 gtcggcgaag agcagaccct gtacgagcag gacgacggtc aggcacaacg tcgccgtcca
    50221 cggtccgacg aggaccgcgg cgagcgcgcc gccgatgagg tgacccgacg tgccggcggc
    50281 cacggggaag ttgatcatct gggcggcgaa gacgaaggtg gcgacgagac cggccagcgg
    50341 ggcggctcga tcgtcgagct cgcggcgtgc gccgcgcagg gaaagtgcga cagcgctcgc
    50401 ggcgacggcc gcggtggcca gggaggtcgg cagatcgagg aagccgtcag ggacgtgcac
    50461 ggcggcctcc ttgcggcttg gcggatcggg cggggcggct gctgtcggcg gggcggggta
    50521 cggtgtccga cagggcgacc cgagcgggtc gaatcccagc catcctcaga tcttattgca
    50581 aatcattcgc aatagtcgac cgtgggagtc accgtgacga cacgcttgca ggcgggcgac
    50641 atcgcccccg agttcaccct cagcgacgac accgaccagt cggtctcgct ggccgacttc
    50701 cgcgggcagc gggtcatcgt ctacttctac ccggcggccc tcactcccgg gtgcaccacc
    50761 caggcgtgcg acttctccga ctccctcgac gcgctcgccg cgcaggggta tgccgtgctc
    50821 ggcatctcgc cggatgcccc ggccaagctc gcgaagttcc gcgccaagga ggggctgacg
    50881 atcacgctgt tgtccgaccc cgacaagtcg acgctcacgg cctacggcgc cttcggcgag
    50941 aagaagctct acggcaagac cgtcgtcgga gtcatccgtt cgacgttcgt cgtggacgcc
    51001 gacggggtca tcgccgacgc tcgatacaac gtcaaggcca ccggtcacgt cgccaagctc
    51061 cggcacgacc tcggcctctg agcgcccgcc cattaggctt tgccccagga cgaagggagc
    51121 gctcgatgag cgagcaggga caatcggtcg aacagctcca agcggagatc gaggcggctc
    51181 gctcccggct gtcccgaacc atcgacgagt tgacctatcg agcgcagccg cagcagatcg
    51241 tcgcccgcca gaccgaggcc gcgaaggcat ccctcatggc ggcgacccac gacgacgacg
    51301 gcggcctgcg caccgagcgc atcgcggccg tcctcgcggt cgtcgcggcc ttggccatcg
    51361 ggctgggcct gctgcgccgg cgccggggct agtccaccgc cgagatgcca cctcgtgccg
    51421 cgtcggcggc cagcacgtct gaccggttgc ccatccgaat gctcttcgat cgggttctgg
    51481 tctccatcga ctccgagaag ggcgagcgca cctccggagg cggcatcctc atccccgcca
    51541 cggcgtcggt cggcaaacga ctggcctggg cgatcgtcgt ggccaccggc ggcaatgtcc
    51601 ggcaggtgca gccgggtgac cgggtgctct acgaccccga ggaccggcac gaggtcgagt
    51661 tgcagtccaa gggttacatc ctgttgcgcg agaaggacat tcacgcggtt gcggcctcgc
    51721 gcgtcgatga cggcaacacg ggcctctacc tctgaagggc gctcgcgagg gtgcttcggc
    51781 agccaggtga cccccaggta tgccgcgagg gcggctcccg taggaaccgc cctcgtgtgg
    51841 tgcgccgcca gggattcgaa ccccgaaccc agtgattaag agtcacttgc tctgccattg
    51901 agctagcggc gctgggccga gccgaaactc tacacgctgg gttgggcgtg gtggaaatcg
    51961 gttcctgcca caccctcctg cgagcgtggc gaacgacgct gcgcctgggt catcgacgct
    52021 tgcggcggct ttcccagacg cccgtgaggc cggccagagc cagcagggcg ccgccgatgt
    52081 agagccaggt cataccgtcc tggcgttgcg atccggtcgt cggtgtcgcg ctggccatgc
    52141 tgtcggtcgt cgctgcgcct gagccggcgc tggggttgct gggggcgctg gtcgtgttcg
    52201 gggcgctggg agccacggtc ggggtgggtc cggtgctggt cggcgtccgg gtggggcccg
    52261 tgacggtgaa actcgtctcg ccggagatgg ggtgtccgtc ggcgctgacg acgcggtagg
    52321 cgatccggta ggtcccgggg cccgggtcgg ccaaggtggc gcgcagcacc gtgccggtag
    52381 cggttgccgg gttgagcgcg acgacatcgc ccgagcgact gagggccagc tgggcgaagc
    52441 gggggttgat ctcctcgttg aaggtcagca ccaactcggt cggccccgac gccagcaccg
    52501 cttggtcggc ggggacggcg ctcacgaggg cgctgtgcgc gggcgcgagc gctgcggcat
    52561 acggcatcgg ggtggccacg gcgctgggag gcgaggtgag tgagccgagg gtcacggcaa
    52621 gccctgccac cgcgacggcc acgaaccgtc caaccaccat cgggtcgccc cttcgacgaa
    52681 cgatgtcgac ctgaacagtc gttcgaacgt cgtgacgctc gaaacgcgcg acgctcaaac
    52741 gcatggggtg agtgacgggg cttgaacccg cgacccccgg gaccacaacc cggtgctcta
    52801 ccagctgagc tacacccacc atggacatga cccgaaagcc acgcacagcc gcctatcgta
    52861 ccgggtcggc aacagtgctg ttgaccgccg ggtgaacgac gacgtgccgc tcgccgagag
    52921 cccgcgccgt tgcggagtcc ggccccggct ggggcacgaa gaccgcctca cggtagtagc
    52981 gcaactccgc gatcgactcg cggatgtcgg ccagggctcg gtgcccccca gacttcgagg
    53041 gggagttgtg atacgcccgg gggtaccagc gacgggatag ttccttgatc gacgagacat
    53101 cgatgacccg gtagtgcagc cacccctcca gctcgggcat gtcccgggcg aggaaggtgc
    53161 ggtccgtgcc gacggtgttc cccgccaaca ccgccttgcg gggttcaggc acccattccc
    53221 ggacgtaggc gagcacccgc tcctcggcct cgcgaagcga gacgcccgtg gggagttcgg
    53281 tgagcaggcc cgaggtcgtg tgcatgtcga cgacgaaagg atccatctgg gccaccgact
    53341 cgggcggggg acagatgacc acgtcgacgc cttcgcccag gacggtgagc tcgtagtcgg
    53401 tgaccagcgc ggcgacctcg accagcgcgt cggcctgcag tgacaggccg gtcatctcgc
    53461 agtcgatcca gacgatccga tcggcgaggg ctcggtcgcc gccggaggaa ccggcggtcg
    53521 gcgaggaggt gggggtggtc gacgcgttgg tgctcatgct cacggcacca ggttagaggc
    53581 cccgggggct gcgctgcgct ggactagtct gtcggccatg tcgacggggg gatctggggt
    53641 gccgggctgg gactcgggga cggcggcata cccgatgccg ggtcctggcg tccccaaccc
    53701 gacgaggcgc ccggaacggc gcggctgggt gacgaccggc gtgatcatct cggtcttcct
    53761 catctcgctc gccatgttgt cggtgttctt cgggatcgac atcgggatcg gcaactctct
    53821 ggtggcggct gcggtctccg cggttccgct cctggtggtc gtgccggtct tcctctgggt
    53881 cgaccggatg gagtccgaac cggtccgcta cctgatcttc gccttcctct ggggagcgct
    53941 gtgcgcggcc gtcggcgcga tcatcctcaa caccgggatc cacctgatct tccttgcggc
    54001 ccacaccgac aacgccgacg cggtgaccgc cgtcgtcagc gccccgttgg tcgaagagac
    54061 gctcaagggc gccgcgatcc tcttcattgc gtggcgacgg cgacgcgagt tcgacggggt
    54121 gatcgacgga atcgtctatg ccgggatggc cggagcgggg ttcgcgttca gcgagaacat
    54181 cctctatctg gggcgtgcct tcgccgaggc ggggacgtca ggtctgaccg aggtgttctt
    54241 cctgcggtgc ctcatggggc cgttcgcgca cccgctgttc acagcgtgca cggggatcgg
    54301 gatcggcttg gccgcgacgg tggcgcgatc cgcggccgct cggatcgcct acgggactgc
    54361 ggggttggtg gccgcgatgg gcctgcacgc gatctggaac ctcagcgcgt cggtgggcgc
    54421 ctacctgcag gtctacctcg ccttccagat gccgatcttc gccgtcttcc ttgtcgggtt
    54481 ggtcctgctg cgccagcgcg aagcggggtt ggttcggcgc tacctgtcgc agtacgccga
    54541 tgccggttgg ttcaccagag ccgaggtcga gatgttgtcc tcgatggggc aacgccggtc
    54601 ggcgcgcgcg tgggcccgcg gtttgggggg acgtgcggcc gtgcggtcga tgcgcgcctt
    54661 ccaggacagt gccagtgacc tcgccctgct gcgggcgcgg atcgtgcggg gttcagccga
    54721 gcccggcgcc ggtgcccatg agcgcgatct gttggacgcc gtgacggcca accgcggggt
    54781 cgtcgtcggc caggtcagcc cccgcgaccg ctgagtgcgt gggtgggcgc cttgtgcccg
    54841 gggtatgccg tccggccggc ttcggaggtt cgtcgcggcg cggggggagc acgccacata
    54901 gactggggcg ccggaccgtc ggctgctccg tcggcccaac tgcccccgta gcacaacgga
    54961 tagtgcagcg gccttctaat ccgcaggttg caggttcgag tcctgccggg ggcgcgccag
    55021 cgcccacggc agaaggcaac aggggctcga acgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    55081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    55141 nnnnnnnnnn nnncgggggc gcgccagcgc ccacggcaga aggcaacagg ggctcgaacg
    55201 caggggacat gaacctctgt gcgaacgtgc gacacacccg gcccggggcg tgtcggggct
    55261 gcatgtgttg agccgggccg atggacttgg aaggatgcgc ctgtgaccgg agcgagccca
    55321 gcgcccggtg agctgtcaat gctgctcgcc gcggtcaacg ggctgcgcga ccaggtggag
    55381 gcctcgcgtc tgccgctcgc gctcccggat gccccggcgg ccggcaaggt ccggctggag
    55441 ttgttgcagc aactggacga ctacgtcatc ccgcggctca gctccctcga cgcgcccctg
    55501 ttggcggtcg tcggaggatc gaccggtgcc ggtaagtcga ccctcgtcaa ctcgattgtc
    55561 gggatgcagg tgaccaggcc gggggtgttg cgtccgacga cgcggtcacc ggtcctcgtc
    55621 caccacccgt ccgacgagca ttggttcgtc gacacgcgcg tgcttccggg cctggcccgg
    55681 gtccgtggcg gtgatctcgc ggaagacccg tccgcggtgc gtctggtcgc caacgacgca
    55741 ctgccggccg gtttggcgct gctggacgcg cctgacatcg actcggtggt ggcgagcaac
    55801 cgtgacctcg ccgcccagtt gctctcggcg gccgacctct ggctgttcgt gacgactgcg
    55861 gcacgttatg ccgacgccgt gccgtggacg ctgttgcgtc aggcatccga gcgcgggacg
    55921 tcggtggcga tcatcctcga ccgggtgccg ccggaggcag tcggcgagat ccgtgaccac
    55981 ctcacggcga tgctcaagga gcagggtttg gcactggcgc cgttgttccc catccccgag
    56041 gtgccgctag gcgccgacgg actcttgggt gacgccgagg tcggccgtct caagggctgg
    56101 ctgagcgcgc tcgccaacga cgcgcgcgca cgggccctcg tggtgcggca taccctcagc
    56161 ggcgccttgg actcgattga ctcgcggatc gccgtcctcg cggaggcgac cggcgagcag
    56221 caggcggcga cgaacgcgct cgcgcgggtc gcggacgcgg catacgccga ggcagccgac
    56281 ggggtgcgca aggggatgct cgacgggacc ctcctacgcg gcgaggtgct gtcgcgctgg
    56341 caggagttcg tgggcacggg ggagttcttc aagcaggtgg agtccgcggt ctcgcggatg
    56401 cgcgatcgga tcacggccgc ggtcaagggg cagcccgctc ctggtcagca actcggcgag
    56461 gcgctgcaga cgggtgtcgc tgcgctcgtc acggccgagg gctcggcggc ggcctcgcag
    56521 gtggctcgct cttggcgggg tatgccgggt ggtcagcttc tgctcgacgc gacccccgcg
    56581 ttggcggtgc cgtccgcaga cctgacgacc cagatcgaaa ccctggtgcg cgagtggcag
    56641 gacgacgtgc tcgacctcgt ccgcaacgag ggccgcgatc gccgaacgac ggccaaggtc
    56701 gcggcctatg gagtcaacgg tcttggcgtc gtcctcatgc tcgtcacctt cgcccacacc
    56761 gccgggttga ccggcgcgga ggccggcatc gctggcggga cggcggtcct cggccagaag
    56821 ttgctcgagg cgatcttcgg tgaccaagcc gttcggtcgc tcgcggccaa ggcgagggcg
    56881 gcgttgctgg atcgggtcga ggacctgtat gccgccgagt cgggacgttt ccacgccgcc
    56941 atcgacgcgg tgtcggtcga gcgtgaccaa gggcgcaagc tgtctgccgc cgccctcaac
    57001 gtcagagccg cccgatgagc cccttgcgga tgggaagtgc cacggtgacg gtcgtgactg
    57061 ctgcggagtt ggccgatcgg gccgctgcgc tgtcgcaggc ctgcaccgag ggcgcccgcg
    57121 agctcgagcc ggcccaggtc gaccaagccg tcaacctcgt cgacaaggtg cgggaacgga
    57181 cgtcgatcgc cggcggccac accgtggtgg ccctggcggg tgcgaccgga agcggcaagt
    57241 cgtccctgtt caacgccatc gtcggtgagc aggtagcccg gatcggcgcc cgtcgcccga
    57301 cgacctccgt gccgtccgcg gcggtgtggg gcacgacgtc ggccaccgaa ctgctcgact
    57361 ggctctcggt gtcctcgcgc caccaggtcg atggcgccac gcggatggac gacgccgatg
    57421 cggcgggttc ggacggggtg ggttcagacg ggttggcggt gaccggtccg gctggaagcc
    57481 tggacggtct ggtgctgctc gacctgcccg acttcgactc gcgggtcgac gcgcaccgca
    57541 tcgaggcgca gcgggtcttg gagctcgtcg acatcttcgt ctgggtcacc gacccgcaga
    57601 agtatgccga cgccctgctg cacgaggact acgtccgtgc gctcgccacc cacgacgccg
    57661 tgaccgtcgt cgtgttgaac cagatcgacc gactcactcc cgaggcggtg tccgcctgcc
    57721 gcgacgacct gcggcgcctc ctggtcgccg acggcctggc ccaggcccgc gtcttcgcga
    57781 cctcggcacg caccggcgcc ggggtgcccg agttggtcca gcagttggcc aacgccgtgt
    57841 ccggcgccaa tgcgacccgc caccggttgt ccgcagacgt cgtgtcggtc gcagggaagt
    57901 tgcgcgctgg ggtggcggac aacgaaatcg acctcgacgg acgagccgac ccggggctgg
    57961 tcgaggcgct gtccagggcc gcgggcgtcc ccatcgtgct ggatgccgtt gcggccgact
    58021 atcgccgcga gtcacgccag gcgggtgggt ggttgttcac ccgttggctc tccaacatca
    58081 aggccgaccc cctcgcccga ctgcgcttgg acaagacggt cgtgccgatg aagtcggtgg
    58141 gggaggccga cgtgcgggcc gtgctgggtc ggtcgtcgat cccgccgccg tcccccgcag
    58201 cccgagctgc cgtctcgctc gcgacccgcc aactggccga ccgggcctcg gaaggccttc
    58261 ccgtgcgctg ggcccatgcc gtcgctgacg cgtcggcgac cggtgacgcg ctgaccgact
    58321 cgctcgacca ggcgatcctg cacacgccgc tgcgtgcccg gccgccccgc tggtggacgc
    58381 tcgtcggcac gctgcaatgg atcttcggcg ccgctgcgat cacgggcgcc ctgtggttga
    58441 ccgcgctcgc cgtggtcgcc tggctgcaac tcccggacct gccgacaccg acggtatggg
    58501 cggtgccctg gccgttcctg ctgttggcgg caggcgtggt tctcgggttg gtgaccgccg
    58561 ggctgtcccg cctggcagcc cgggtcggtg ctcgacgacg ggccgcgaag gcccgcaagc
    58621 ggctgcacga tgcgatctcc gccgtggcgc tggatcggat cgtcgggccg atcgctgctg
    58681 tgttggctcg acatgcccgg gttcgggagt tcctcgatcg ggccgccgcg cgctgacccg
    58741 cgggtcgcgc gacgtcgggc tcgtgcggtg ggtgttgtcc acacccccgt ggtcgttcgt
    58801 cccatcgtcc acaggctggc gatggggtat gccgcgtcgc cgccttcccc ggagaggctg
    58861 tgcctagccc gttcgcaatg gacggggcgg cataccccct ccaggtagag gacacccaca
    58921 tgtacgagag ctacgtgacc gtcgtcggca atctgaccgc cgacccggtg ttgcgcacca
    58981 ccaaggccgg caagcctttc gcgaccttcc gcatggcgtc gaccccgcgc cggtgggacg
    59041 gccgcgagcg agcctatgtc gatggcggca ccaacttcgt gtcggtggtg accttcaact
    59101 cgttggccgc caacgtcgtg gcgagcatgg ccaaggggca gcccgtcatc gtctacgggc
    59161 ggctgcgggt caacatgtgg agcaccgccg agggccactc caacacgtcg gtggagatcg
    59221 acgcctacca cgtcgggcat gacctcatgt ggggccagtc gctgttcacc aagtcgccgc
    59281 gggcccagtt cgacacgacc gaccgactct ccgagccgga gatccagcag gcgttggcgg
    59341 aggacgtgga cggaccgtcc gacatcgagg atgaggaccg tgagccggac tggtccccga
    59401 tcaacatcga cttcaccgcg gacgccgcgg acccggtcgt catcgagggc gcgcccctga
    59461 cgggcgcttc gtcgtggtcc ggtgccaatc ccgagaccga cgcctacgtc gtcgcgcacg
    59521 cccgctgacg gcatacccac ccaggcagcc ggtcgggtcg tgacaacctt cgcgatccac
    59581 ccccgcggcc cgaccggcga ttgggtgcgg caggtgcgtg acggatagcc ttcgggaatg
    59641 gccgagttca tctacaccat gaccaaggcg cgcaaggcgg tgggcgacaa gctcatcctc
    59701 gatgacgtga cgatgtcgtt cttcccgacc gccaagatcg gcgtcgtcgg gcctaacggc
    59761 gcgggaaaat cgacgatcct gcggatcatg gcggggctcg accaaccgtc caacggcgag
    59821 gctcggctct ctcctggcta caccgtgggg atcctcctgc aggagccgcc gctcaacgag
    59881 tccaagacgg tgctgggcaa cgtcgaggag ggcgcgggcg agatcaaggc caagctcgac
    59941 cgctacaacg ccatcgccct ggagatgggt gagccggacg ccgactacga ggtgcttggc
    60001 gacgagatgg ccaagctcca ggacgagatc gaccatgcca acgcgtggga cctggacagt
    60061 cagcttgagc aggccatgga tgcgcttcgg tgcccgccgg gcgacgctga cgtgaccgtg
    60121 ctctccggtg gtgagcggcg tcgggtcgcc ctctgcaagt tgttgctgca gaagcctgac
    60181 ctgctgttgc ttgatgagcc gaccaaccac ctggacgccg agtcggtgca gtggctggag
    60241 cagcatctcg cgcaataccc cggtgcggtg ttggcggtca cccacgaccg ctacttcctc
    60301 gacaacgtcg cgcagtggat cgccgaggtc gaccgcggcc ggctgtatcc ctatgagggc
    60361 aactactcga cctacctgga gaagaag
//
LOCUS       DAORPW010000002        51556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_78_length_51556_cov_816.345508, whole genome shotgun sequence.
ACCESSION   DAORPW010000002 DAORPW010000000
VERSION     DAORPW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 51556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51556
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78_length_51556_cov_816.345508"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLB86_00335"
     CDS             complement(<1..262)
                     /locus_tag="PLB86_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HRC63611.1"
                     /translation="MSPATSLRRHREPAALVLEDGRTFLGESFGAVGETLGEAVFSTG
                     MTGYQETLTDPSYHRQVVVMTAPHVGNTGWNDEDDESSRIWVA"
     gene            complement(259..1590)
                     /locus_tag="PLB86_00340"
     CDS             complement(259..1590)
                     /locus_tag="PLB86_00340"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRC63612.1"
                     /translation="MTDLLLKGANLLGDNGADVLVREGVIAEIGSIPAAGELDGRRLT
                     VVDAAGLVLLPGLVDLHAHLREPGREDAETVASGSAAAAAGGFTAILAMANTSPVTDT
                     AEAAERVFDLGRAAGLVDVQPIGAVSKGLAGVELAELGLMARSRARVRVFSDDGKCVH
                     DARLMRRALDYVKAFGGTISQHAQDPRLADGSACCHEGEVSGRLGLPAWPAIAEDVVV
                     ARDVQLARHTGSRVHVAHVSTAASVEIIRWAKAQGIAVTAEVTPHHLALGTDLLTGYD
                     PVFKVNPPLRPDEDRAAILAALVDGVVDAVATDHAPHARHDKEHAFPDAAFGMLGLET
                     ALAVVAEVAVRTGLLSWADVADRMSFAPARIAGLADQGRPIAVGEPANLVLVDPDASW
                     TVDRSTSQSLSRNNPWHGRTLPAPVVATFLRGECTAYRGRVRGQFDPEEIA"
     gene            complement(1587..2555)
                     /locus_tag="PLB86_00345"
     CDS             complement(1587..2555)
                     /locus_tag="PLB86_00345"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRC63613.1"
                     /translation="MTRKHLLSSADLSRDEVDSILDTAASMHDVQRREVKKLPTLRGR
                     TVINLFFEDSTRTRSSFEIAGKWMSADTINITGKGSSTSKGESLRDTVMTICAMSVDA
                     LVIRHSASGACHQVAQWVDASVINAGDGTHEHPTQALLDAYTLRQRLGSLEGRHIVIV
                     GDLAHSRVFRSNVITLGRLGARVTVVAPPTLMPSGIGDWAARDGFATSWDLDQTLYAA
                     SPADRPDAVMMLRVQRERMSGGFFPTEREYTAGYGLTRERLARLYAATPDLPILHPGP
                     MNRGLEISAEAADATSSAILDQVSAGVAVRMAVLYHLLAGETEAAA"
     gene            complement(2552..3130)
                     /gene="pyrR"
                     /locus_tag="PLB86_00350"
     CDS             complement(2552..3130)
                     /gene="pyrR"
                     /locus_tag="PLB86_00350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRC63614.1"
                     /translation="MVASDQTPSPTPPGARVVMSSGDVSRALRRVAHEILERNKGPAD
                     LVLLGIPSRGVPLAHRLASVIAEVEGVAVPVGSLDVTMHRDDLRRQPTRTVTPTKIPP
                     SGVDGKIVVLVDDVLYSGRTIRAALEALTELGRPRAVRLAVLVDRGHRELPIRADHVG
                     KNLPTSSSERVHVVLAENDGGDDEVRISGGDR"
     gene            3319..4338
                     /locus_tag="PLB86_00355"
     CDS             3319..4338
                     /locus_tag="PLB86_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HRC63615.1"
                     /translation="MSSDTPASEYRIRATAEDVRRMMSRLLLTEGSDTRRKMSAFWVL
                     LVLATVIATSGVVADSTATVIGAMIVAPLMTPILGTALSVVLANRRLVAVNFGLVIVG
                     ALCVIAIAYLIGMTSQMHLVADSNSQIAGRVSPRLIDLVGALATGAVGAFATVRSDVS
                     DTLPGVAIAISLVPPLAVVGLTLESGEPSQALGALLLFGTNVSAIIATGTVVFLSYRV
                     RQAAIDAGLPIGRLHRGALVTAFASVVILAIPLSLGSSVVLQQEVLVSKATPVARAWA
                     KAHGGQISDVTYRDNSLRILAIGLLTLDQVTGLRPDLDAAGLGDVDVLVTLVAGGTKE
                     LPGKP"
     gene            4440..5783
                     /locus_tag="PLB86_00360"
     CDS             4440..5783
                     /locus_tag="PLB86_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRC63616.1"
                     /translation="MQARQVRPLDAAYLALDGPRTVGHVCLVLPLDGPITLTQLREQV
                     GERVGRLPELRQRLHQGPNALDRPWWVDDPTFDIEAHVEEFHVGRRELSELVSELAME
                     ALPRTQPLWQVHLLREGRRCYVMTKVHHSIADGSRYRDILHALFGTAQDDVPPWSPQR
                     IPSAFDLATRGLVGASRWAWTSVYAAARAIADNPQSLGGVLNSVGVHGAPPTPFNRSV
                     GPNRVWAFRTWGLAASKPTRAHFGVTVNDVMHAVVAAGLRQWLIEQNALPTSPLVALV
                     PMSVRHLTTDPSGANRLSLGLCRIPTDIEDPVERIFSAREDMAEAKERPVLGEGALEV
                     LTRLLGPTLEPASWVASTVRLLDTVRLPFNTIISNVPMGQDLFVIAGRRVTAVYPFAP
                     LGDGMGVSITIQGYQGRLDVGISACADLLPDVEHLMDLMTAEYAHVCTLASLTGG"
     gene            complement(5803..6300)
                     /locus_tag="PLB86_00365"
     CDS             complement(5803..6300)
                     /locus_tag="PLB86_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63617.1"
                     /translation="MGFLDKAKAAATELSAKADQALASQGLAGPSVGGKQADKFFRDL
                     GVLAYLDATGRPSDAGERDRIMTALQGFEAQGALPSFALQTAAPPPPPPPGAAAQAPQ
                     GYAAAPPPPSPGGAAQPPPPPGWGAAPPPPPPPPNWGAAPPPPPPPNWGAAPPPPPPS
                     PPTAG"
     gene            6453..7133
                     /locus_tag="PLB86_00370"
     CDS             6453..7133
                     /locus_tag="PLB86_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HRC63618.1"
                     /translation="MAIHGSLFTQYQENPSADPFVLQNKKLLKISMQYGPVFAKLGSM
                     VAYQGDVRFENPGSGGLNKMFKSAMTGEGVKMMRCTGAGELFVADAAAEVQVFYLEND
                     MVSVNGNNVLAFSSSIEWDIHRIQTRGMMTGGLYNVSLRGTGYVAITTKGDPVALDVA
                     GAPTFGDAQAVVMWTSGVSMDIRVDTGGLSSMIRGGTGETFQMAFGGQGYVLIQPDEG
                     VVSGGHQA"
     gene            complement(7165..7578)
                     /gene="nusB"
                     /locus_tag="PLB86_00375"
     CDS             complement(7165..7578)
                     /gene="nusB"
                     /locus_tag="PLB86_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRC63619.1"
                     /translation="MGARTKSRKRALDILFEADQRGINAQQLLIERLARPATPAPLAD
                     YTITLVEGVVRCWSEINEMLATYSQGWSVERMPAVDRAALRLATWEIVYSDEVPDAVA
                     IDEAVELVKSLSTDESPAFVNGLLARIAEVKHTLG"
     gene            complement(7584..8147)
                     /gene="efp"
                     /locus_tag="PLB86_00380"
     CDS             complement(7584..8147)
                     /gene="efp"
                     /locus_tag="PLB86_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRC63620.1"
                     /translation="MATTNDLKNGLVLNIDGQLWAVIEFQHVKPGKGPAFVRTKLKNV
                     LSGKVVDRTFNAGVKVETANVDKRTMQYLYKDGSDFVFMDTDTYDQLHVSEAVVAGGA
                     DYLLENQNAIVATHDGVPLYVELPASVVLEITFTEPGLQGDRSTGGTKPATLETGAQI
                     QVPLFLESGTRVKVDTRDGSYLGRVND"
     gene            8579..9238
                     /locus_tag="PLB86_00385"
     CDS             8579..9238
                     /locus_tag="PLB86_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRC63621.1"
                     /translation="MFRITIPTRHAAVVYHAGRLVDVLPAGRTRRRWGHHYVLVDLRE
                     RQQVIPPQDIPTADGLGVRVSAVARWAITDPVAFLEVSQTPADTLHLATQLALREAVG
                     ALAAADLVVALRRLDVTAPVDAATRQVGIATLGVAVRDVVLPHDLRIATAEVVTARLR
                     GAAALEAARAETAALRALANGARLLDEHPALAQLRLVQSAPPGTRLVLQLGGTPEPAG
                     D"
     gene            complement(9335..12034)
                     /locus_tag="PLB86_00390"
     CDS             complement(9335..12034)
                     /locus_tag="PLB86_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63622.1"
                     /translation="MYKKGVGEFKFYVGISSLAQIATKDDRVCVLNILGGESSDVTPV
                     SHEYSGGNVVFGTSPGKGGSHMPTNLGDIPVYNNVLEGLADGHRFNAGVVYLPPAAAR
                     HGVGELIRVNPEMRKIFIVTEKIPAHDAREIRAMAQSNGVDIFGANSLGVADSWNHVR
                     IGGALGGNTPEEALRKGSIALLSNSGNFTTTIATYLRMAGWGTTTLISSGKDVYIHYA
                     APEFAFALANDMRSKAAVLYVEPGGYYEWDAEFTKPVVACVVGRWKSRLTRAVGHAGA
                     MAGGGDDAATKERWFMEKFGVEDAFTPDNPVCSRRGAVVTNIAHIPAALTAVMALNAT
                     MPDFAPEGSLALKPWFGRARDLELPPELDLPNVVAMPPYDQQIAELNRQIGAVLPRQS
                     MKDASGVSQMDPLTQVSSLRGMSILQAAQHPMEDNLVLALLGRRASERDRALVDVAVG
                     AHVNLAGTVTLAAVDAARAAGNTPNTVIAAALAIAGPGRAEASRHAIHGFIDLFTGSG
                     LSVDDTETDLAAYPAPPELRALAIGADPDPIAEAMLAGLAERGVTSMPLRWLATQGGH
                     PQVPGVVAAIAGTLVWKSLAHKEISRITAEELPWWLELFGTMIGAAAPTAAHTAEGFC
                     GIPMAQVLGQASVTELAAAALLGERPSADILDGFAMLTGLLLTNGPGAITAQGAKGAV
                     SADGPQTPERIQLNKAMIGFLTHAGYAHGGNGFEGIAFLLERFAGTGLVDPGDPAHGV
                     DLDALAKEYAVAFAELKTTRKELGADRATIPCINHPVFKGKDVNVDPREEFVRTVMAQ
                     RGDYNVFHDYYRRLVVALRDAGATTNVFCVNVDALIATGLLKMLWSRYRDGSFTEGQL
                     ENAAFTVFLFGRMIGCAAEIDDHQNRGRNMDTRSPAKALSFVS"
     gene            complement(12059..13336)
                     /locus_tag="PLB86_00395"
     CDS             complement(12059..13336)
                     /locus_tag="PLB86_00395"
                     /inference="COORDINATES: protein motif:HMM:NF020031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HRC63623.1"
                     /translation="MNLTGMLYGAKLLQFAGFPAAEVLGPAATEEEIKDLISRHGEIL
                     IKPVFKGGVGKKGKAGLIGRAKDLPTALREKERLYFAEHQVGTTLAKANGVTYEAMIP
                     AEHEVYFSLTDSTHFRAPTMTITHHGGVAIEELDKSLVASVPFEALTGMRAFVVSNAL
                     SQLQAPSEIVSPLVQHLPSLWELFHEFGMTTLELNPIRMRADKRGRLTPVACDFKCAF
                     DRDDPRVARLDLPRSLFSSSYSDFEHEVNQLRTYQGQSDVYVVNEKGTILAPTFGGGA
                     NSLVSEMLGDDAIISSDFGGNPPYVKMKELARICYKYWLPQTNVLFIIGGKANNTDIF
                     ETFRAMADALREHYATHGPTPLYAVIGRGGPNLIRGMGVLRDTFESLKIPYKMFGFDS
                     DMSEVINYARAADAWMKASGREQIARQQQALAG"
     gene            complement(13408..14064)
                     /locus_tag="PLB86_00400"
     CDS             complement(13408..14064)
                     /locus_tag="PLB86_00400"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC63624.1"
                     /translation="MSNSPKAARKQRDAARTQADLLTVATEEFARIGYFGARVDEIAA
                     KSATTKRMIYYYFGDKEGLFTAVLEKAYADIRETEQALDLAHMPPLDALTVLIRHTFD
                     YHEAHPGLARLVASENALDASHLRLSQRAAGVNLPIIALVADLLARGRSEGSIVRDVD
                     PLDLHLMMTSMALFRITNAPTIEATFGLRMGTHEHKERQIALVTEMVLTWLTTPDSRD
                     "
     gene            complement(14153..14335)
                     /locus_tag="PLB86_00405"
     CDS             complement(14153..14335)
                     /locus_tag="PLB86_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63625.1"
                     /translation="MSSTTNPIVGLVVGVLLIPLGWYVARDGGASMGPFGWLLLGVGV
                     ASTVGNGLLMLRARRR"
     gene            complement(14448..15854)
                     /locus_tag="PLB86_00410"
     CDS             complement(14448..15854)
                     /locus_tag="PLB86_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63626.1"
                     /translation="MSTSHGVTHDHGQTKKATASGWIGSALEYYDFFIYAQAAALVFP
                     SIFFDKNDPKMGIIGALGTYAVGYVARPIGAFVLGAWGDKHGRKNVLVLCMLLMGAST
                     FLVAILPTYQQVGFLAPALLLVLRLVQGFAVAGEISGASSMILEHAPFGRRGYFGSFT
                     LQGTQFGQILAAAIFLPLATFMSDESFKSWGWRIPFLLSVFVVFAGYIIRRRVEETHA
                     FESEKAQGNVAQTPIAEAFRDSGMSILRVFMMQLFNIIAVTTSVFGATYATNKEYGVG
                     MAASVYLWIPILGNICAVAVIPFVGNLSDKIGRRPLIMFATITCGLLSFGYLWSVSQK
                     NVPLAIAFSLLMWGTVYQGQNAVYPAFYPEQFPTRTRVTGMAIAHNMGTAISSAMAFV
                     LAWVAPPGTQNVWLVVGAITMGICIISFFGAYFSPETSRFRSEDLGVPGAKPVPEAEY
                     LRLRQAAIDSAKAQKVAA"
     gene            complement(16014..16880)
                     /locus_tag="PLB86_00415"
     CDS             complement(16014..16880)
                     /locus_tag="PLB86_00415"
                     /inference="COORDINATES: protein motif:HMM:NF009201.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRC63627.1"
                     /translation="MSTEPLVTHSLFLIGEGIGKSRTPALHEREGASLGMPVSYRIVD
                     ALELGLTAADLPDVLTWAKRLGCSGFNVTHPFKQAIIPYLDELSDRATALGAVNTVAI
                     RDGRTIGHNTDWCGFQRPFQAQLPDVGHGAVVQVGSGGAGAAVAYALLDHGANPLILH
                     DFFPERAQELADRMAAIFGADRVEVGTDLAASMARAEGVVNCTPTGMATHPGCAVPEE
                     LITPEHWVAEIVYFPLETELLRVARAKGCRTVDGGGMAAEQAVEAFYQFTGVHPDAQR
                     MHRHLRDMVGGA"
     gene            complement(16961..17785)
                     /locus_tag="PLB86_00420"
     CDS             complement(16961..17785)
                     /locus_tag="PLB86_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRC63628.1"
                     /translation="MGTAAPQRYSLAHLTILDTPTPELVRIAHRTGYDYVSPRLICHG
                     LPGDDYSLATNPTLLAQTRAALADTGIPVHDIELARIVEGLDPRVYEPEIAVGAELGA
                     TGVLTSIWVPDRPFYLDAFGRLADLVASYGMTLNLEFVPIAAVRNLAGAVDVLRETQR
                     PNARLMIDLHHFHRSRDRVEDLDGLPPEWFEFCHLCDAPGEIPADVEKMTHILRAARS
                     YVGEGGINPAATLAHLPPLIYSIELPNEREEVERGAEGHARRCLETAKAYFAQPAA"
     gene            complement(17795..19561)
                     /locus_tag="PLB86_00425"
     CDS             complement(17795..19561)
                     /locus_tag="PLB86_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRC63629.1"
                     /translation="MTTDLVSHQLVRYLEHRGVRHVFGLCGHTNIAVLAALSRSTQIE
                     FVNTRHEQIASHAADGYARATKKASVVLSHLGPGLTNAATGVANAALDSIPMVVIAGD
                     VPSHYYGKHPHQEVNLHADADQWQIYRPFVKRAWRVDSAHLFPEILDKAFALAESGRP
                     GPVLVDVPMDIFSAQIDVALMDRVMTGTHELVRPSLDEATAETIVGNLLAAERPLLYV
                     GGGVVLADATDELAELVDHLQIPVAHTLMGKGAVADDNPCVLGMTGFWGTEFINDMTR
                     NADWILALGTRFSEADSSSWEEAYTFRMPPTRLMHIDIDPAELGRNYPLALGAVADLK
                     PALAALVRAAKKLAPAGVQRTEVLADIQRNRAAFKATCQEGASSDAWPMRPERILADV
                     RAVLPRDAIITTDVGWNKNGLAQQMDILTPGSVFTPGGFATMGFGAPAALGAKIACPD
                     RVVVSLVGDGGFGQNPALLYTAREKGIAAIWVVMNNNAFGTIAGLEKAHYDTTYGTVF
                     ENADGPVETDFAAIAAAYGVAGIRVETAADFRPALERAVAMNVPVVIDVRMVNVPTPT
                     AGHWNIMDIYSPGKSIHHASTD"
     gene            complement(19558..20439)
                     /locus_tag="PLB86_00430"
     CDS             complement(19558..20439)
                     /locus_tag="PLB86_00430"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: protein motif:HMM:NF004864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HRC63630.1"
                     /translation="MSERTAQQLEAVVESAQDWWSTDIVDIHPGYIAMRGYPIEELIG
                     HVTFPEMIWLMLRGELPTRPQARLMEAALVSAVDHGPHAPSIAISRMAMTCGVPLNGA
                     MASAINVLDDVHGGAGQQCMELYSEIAARVDGGAALATATAEVLGEWRTQGVRYVPGF
                     GHRWHPLDPRAPRLLGLVDDAVAAGDVSGRYAAIGRAVEDAISAGRPARIPMNIDGAT
                     AVIYSELGFATELGRGIFILSRSVGILAHAWEQHSQGGRIKGPMPKTIPYRYTGPARR
                     AVPDLDLTEPTADKESR"
     gene            complement(20520..21959)
                     /locus_tag="PLB86_00435"
     CDS             complement(20520..21959)
                     /locus_tag="PLB86_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRC63631.1"
                     /translation="MADKKELTADQQAELDEIFARARAALAIIETYDQARVDRLCQAV
                     AWAVSNKVTFTRLVAEGIEESGLGDPVSRMGKRMKIRGVLRDALRQKSVGIIEEIPEK
                     GLVKYGKPAGIIGCIVPTTNPDLTPAGNAIYAIKARDVVIFSPHPRSKKTTFETVRLM
                     REALEREGAPADILQCITLPSIPVSQALMARSDLVIATGGQPMVRAAYSSGTPAYGVG
                     AGNSTMIIDETADVAIAARNSRISKTSDFGSGCSADGNLIIHESIWDEMITALQAEGG
                     YLAEGSERDALAAVMWDAEKHRLPDSVAVAPQKLARLAGFEIPTDRTFIFVKGDGISR
                     EHVFSMEKLTTLLAVHRYEGEFENALDAMRAIYEVGGKGHSCGIYSHDADHIHRLGLA
                     APVSRIMVRQPQSKANAGAFNNGMPMTSSMGCGTWGGNITSENVHLKHYLNTTWVSVP
                     IPEDKPSDEELFGDFYDATLEPEGEVITS"
     gene            22174..22812
                     /locus_tag="PLB86_00440"
     CDS             22174..22812
                     /locus_tag="PLB86_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRC63632.1"
                     /translation="MSHQSSAALLALHAVRVTGMASTERAAARYGLPRAETHELLEDD
                     RARGYVRRVEFVDLAGWTLTESGRTEDERRLAAELALTGARPVVQAGADDFVGLNARL
                     LTAITDWQLRPQPWDRLAANDHTDHRWDDRVLRELTRIGELLAPVCDPLAAALSRFEG
                     YPQRYAAAMARVADGERSWVDRVGPDSAHGVWLELHEDLRATLGAGPCVTPS"
     gene            complement(22799..23239)
                     /gene="aroQ"
                     /locus_tag="PLB86_00445"
     CDS             complement(22799..23239)
                     /gene="aroQ"
                     /locus_tag="PLB86_00445"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRC63633.1"
                     /translation="MSVLLINGPNLNMLGTRQPEIYGSTTLADVEAAVRAQGEAAGVS
                     VECFQSNYEGAIVERLHAARTDGTAFIIINPGAFTHTSVAIRDAFAAAEVPFAEVHIS
                     NVHKREPFRHHSYLSDIAVGVMAGFGTLGYNLAMTFALQELKTA"
     gene            complement(23303..24385)
                     /gene="aroB"
                     /locus_tag="PLB86_00450"
     CDS             complement(23303..24385)
                     /gene="aroB"
                     /locus_tag="PLB86_00450"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRC63634.1"
                     /translation="MADLVRVGVGDDYDVLVGRGIATFAVDLLADWVAKVLVVHAPTG
                     RDLAALLAARIEAGGRTAYLHEVPDAEAAKTPIVAAQAWGVLGQAGFTRSDAVIGVGG
                     GAVTDVAGFIAATWLRGVAVVQVPTSLVGMVDAAVGGKTGINTAEGKNLVGSFHPPAG
                     VVCDIATLSTLPRADLVAGMGEVVKHGFIADPRTLELIEADPAAALDPAGDVLAELVE
                     RSIRVKAAVVAADLRESSLREILNYGHTFAHAVEQVEGYSWRHGNAVSVGLVYAAALG
                     RLAGRTPADVAARHTEILTSLGLPVTYPSAGGQAWPRLLTAMRRDKKSRGALLRFVVL
                     DDLATPGRLEGPDEALLEAAFAELAS"
     assembly_gap    24516..24615
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24616..25829)
                     /locus_tag="PLB86_00455"
     CDS             complement(<24616..25829)
                     /locus_tag="PLB86_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/3-dehydroquinate
                     synthase"
                     /protein_id="HRC63635.1"
                     /translation="MIVVVGFMGAGKTTVGRLIAERLGLPFVDSDLVIEHQQRRSIKD
                     IFAAEGESGFRDVEAATIAELLDGPACVLSLGGGACGRAETRERLRGHTVIYLHVELD
                     EALARVGRDEYRPLLHDAGLPALYAARLDIYAATATITSRTTGRRVEDIGLEIIDAIT
                     GTAALDGTAGVLVAPGGGSYRVHVGRGIRSQIAALLPPMPDAEQIVILATADEGEAAA
                     EIVAQLRTLAIPVRRVTLPSGSTAKTLDAVGLAASALAELSVHRGDLLVGVGGEATCD
                     VTGFLAATYNRGMAHALVPTTLEAQADSAIGGKCSIDLPHGANLLGTIHQPVLVVCDI
                     ELAVLSATYGDADYRAGLAEIVKHALLEDPALLDTLRERRDAIVARDGQTLVEIVRRS
                     VEIKAAVVTADER"
     gene            complement(25843..27066)
                     /gene="aroC"
                     /locus_tag="PLB86_00460"
     CDS             complement(25843..27066)
                     /gene="aroC"
                     /locus_tag="PLB86_00460"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRC63636.1"
                     /translation="MLRWLTAGESHGPALIAILEGLPAGVSVSRADLGRALARRRLGF
                     GRGARMAFEEDDVEFLGGVRHGFTLGSPIAVRVGNSEWPKWQTVMSPEPVDPDAYAAA
                     NDVNAEKEIARNRPLTRPRPGHADLVGMQKYGFDDARPILERASARETAARVALGEVA
                     ARFLEQTVGIRLVSHTVAIGTAAVADTAALPHPDDVDRLDADPVRTLDAEGSAAMVAE
                     VEAARRDGDTLGGVVEVLAYGVPPGLGSHVHWDRRLDARLAGALMGIQAIKGVEVGDG
                     FETAKRRGSAAHDEMARRDDGVIERRTGRAGGTEGGMSTGQILRVRAAMKPISTVPRS
                     LDTIDVASDEPAKAIHQRSDVCAVPAAGVVAEAMVALVLAEAVVEKFGGDSVAETARN
                     HAAYLAAVPERMRTW"
     gene            complement(27124..27798)
                     /locus_tag="PLB86_00465"
     CDS             complement(27124..27798)
                     /locus_tag="PLB86_00465"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRC63637.1"
                     /translation="MTVFTQTGPWWLVLAAVVVGSALGGWLRRELVTGGYRLDDEWDR
                     PVLRRPWLLVALVPLVWGLLAWRVGGPGTLTLLPALLLVGFAGVALSWIDIDVHRLPH
                     GLTYPLGAGVLGIVLVAGAVSGSWTLPVRALVSGVAAYLVLLVLALISRGQFGLGDVT
                     VGGILGIALGSVDGRLPWWGLMLAFVISGLVSVVGLATRRLTMRTDLAFGPYLLVGAL
                     VAVLLG"
     gene            complement(27824..28645)
                     /locus_tag="PLB86_00470"
     CDS             complement(27824..28645)
                     /locus_tag="PLB86_00470"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRC63638.1"
                     /translation="MRSVVHNRCGVRGSPIEHSLSPVLHRAAYRALGLGDWSYDRIRV
                     EADELATHVAALDETWRGLSLTMPLKEAAFDVVHTASDLAVQVGAINTLVRDEAGWSG
                     HNTDVYGIVEALREAGVGHVRSAVLVGAGATARSALAALADLGAAQVSFMVRDAVRPG
                     TLAFARDLGLDAVTVPMGHWPYGTDVIVSTIPATAYAGLLGALPEAHPGSAVLDCVYG
                     DGPSPLLGVAQEIGYAVVGGTDMLLHQAREQVRLMTSRDAPTEAMRAALLAAADV"
     gene            complement(28642..30042)
                     /gene="mltG"
                     /locus_tag="PLB86_00475"
     CDS             complement(28642..30042)
                     /gene="mltG"
                     /locus_tag="PLB86_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRC63639.1"
                     /translation="MTERPLRDPRGDDVWGQAAGEVAGEHEVHAGHEGYDAHEVDYSG
                     AGGYAHEGPDVHEGEYAHAGNDAHEGEYAYEHEYEHDGVEEGHGGYHDPDAAAFGFAE
                     DSRRARRERRNTGRGVRRFLILFVALGLIAGAGFLGFTALRPLFDRSADASDFAGPGG
                     AAARFTVNDGDTGRAIGSNLQTQGIVLTAKAFVDACSADSRCAGIQPGDYALKKEMKA
                     ADALAILVDPKNRQNPKVTVREGLWASEIYALLGKATKHSVADYEAAAKSPDVLALLP
                     PSAGGNVEGYLFPATYEFSDKDSAVTQLTAMVGKTVKVLTDLQIQPDQAERIMIIASI
                     VEAEAKRSEDRAKVARVVLNRLAKPMRLQMDSTVSYGVKKRAITTTDEERASVNGWNT
                     YTKDGLPDSPIANPGLASIQAAVAPAEGPWLFFVAVNPETGETKFAATQAEHDTYVAQ
                     FQAWCNAAENQGKCSR"
     gene            complement(30039..30539)
                     /gene="ruvX"
                     /locus_tag="PLB86_00480"
     CDS             complement(30039..30539)
                     /gene="ruvX"
                     /locus_tag="PLB86_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRC63640.1"
                     /translation="MPSQGRRVGVDVGSVRVGVAESDPDGILATPVTTLARDESPEHA
                     DLAALAALVRDRDATCVYVGLPLQLSGVSGESARQARAYAAELAALVAPVRVRLVDER
                     LTSVASHRLLHDSGRAERTHRAVVDQVAAVLILESALEAERATGSQPGELVSTGGRKP
                     RTKGRA"
     gene            complement(30546..33248)
                     /gene="alaS"
                     /locus_tag="PLB86_00485"
     CDS             complement(30546..33248)
                     /gene="alaS"
                     /locus_tag="PLB86_00485"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRC63641.1"
                     /translation="METAEIRRRFLAFFEGRGHTVVPSAPLLYDDPNLLFVNAGMVPF
                     KPYFLGQETPPFDRATSVQKCVRTGDIEEVGKTSRHGTFFQMNGNFSFGDYFKKGAIK
                     AAWELLTTPQQQGGYGLDGDRLWATVYLDDDEAADLWVKHTGIPRERIVKRGMADNFW
                     SMGVPGPCGPCSEIYYDRGPDYGREGGPAVDEDRYLEVWNLVFMQNIRGTGGAKEGYE
                     ILGDLPAKNVDTGMGLERMAAILQGVDNLYEIDEVYPVLDKAAELTGKIYGANSGRDA
                     GHSHPDDVRLRVVADHVRSSLMLIGDGVTPGNEGRGYVLRRMLRRAVRSMRLLGYDDP
                     ALPHLLPVSMERMSKSYPELRTDFGRISTIAYAEEEAFRRTLASGTTLLDGAVARLKG
                     TGGEVLTLPGDEAFALHDTYGFPIDLTLEMAAEQGVQVDEAGFRRLMKEQRDRAKADA
                     KAKKSAHGDTGVYREVADRLGRGVEFTGYDEVVSEARVAGIVRDGRSVGQAIAGEDIE
                     LVLDRTPFYAEGGGQLADGGRIELANGAIVQVRDVQSPITGLSVHRATIIAGEVIVGE
                     QAQAEVDVERRRSISRAHTATHMVHKAIREALGDTATQAGSENGPGRFRFDFNAQHAI
                     PLGQLQEIEARVNAVLLDDLPVSAEVMTQDQAREIGAMALFGEKYGNAVRVVSVGDWA
                     RELCGGTHTPRTSQLGVVKLLGEASIGSGVRRVEALVGADAYSFLAREHAIVGQLTEA
                     LKARPEELPERISGILAKLRDAEREIAAARQQQALAVAPGLVSSAREIGSVRLVTHDA
                     GEGIAPDDLRSLVLDVRSRLGDAHATVVAMTGSMGGKPVVIVATNAPARQGGVKAGAL
                     VRLAAQVLGGGGGGKDDLAQGGGVDAASMGASLGAVEDSLRAQG"
     gene            complement(33360..33659)
                     /locus_tag="PLB86_00490"
     CDS             complement(33360..33659)
                     /locus_tag="PLB86_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63642.1"
                     /translation="MSRKLLVLATGALVGVGAVYAYRLLADRYSTEGGPPDVLAAALA
                     EAGAVVADIRAGMTEREEQLRLALGLDSPPSGTRLDPQATRDLLDDPAGWRARQG"
     gene            complement(33656..34054)
                     /locus_tag="PLB86_00495"
     CDS             complement(33656..34054)
                     /locus_tag="PLB86_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HRC63643.1"
                     /translation="MTIGDIAGVIAALAFAYLVWRLGSVVGKAGEILDETRIGVKGLA
                     DGSVPLLGEVTTTVSTTNEQLARIDVVTTNIASMSSNVNALTSLFAATLGSPLVKVAA
                     FSYGVRSAVGQRRATTAASGGSTRKGRHEQ"
     gene            complement(34133..35197)
                     /locus_tag="PLB86_00500"
     CDS             complement(34133..35197)
                     /locus_tag="PLB86_00500"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRC63644.1"
                     /translation="MTANPGSASAPLLEVEHLVKYFAVREVQGLTMVRNTVKAVDDVT
                     FTLAQGETLGLVGESGCGKSTVSRCLTRLETPTSGAIRYKGRDIATLPEKQLRPLRRE
                     IQMIFQDPYSSLNPRQTVGAILSTPLRVHGLAKGKEKARSQELLELVGLNPEHINRYP
                     SEFSGGQRQRIGIARALAVEPKLIIADEPVSALDVSVQAQVMNLLSRLRSELGLSFIF
                     IAHDLGVIRHFCDRVAVMYLGKIVELGNKQHIYGTPQHPYTQALLSAAPDINVVRGIP
                     PKERIRLVGDPPSPISPPSGCRFRTRCWKAQEICATQEPPLEDKPGGGDDDVPGTGDE
                     HVIACHFPEIKSTLVAEVTR"
     gene            complement(35194..36219)
                     /locus_tag="PLB86_00505"
     CDS             complement(35194..36219)
                     /locus_tag="PLB86_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC63645.1"
                     /translation="MTEAPAGEVVLKITDLCVRFPSDEGPVNAVQDLTYEARLGRTLA
                     IVGESGSGKSVSSMAVLGLHNPKRSTITGSILLDGNEIVGADESLLRRLRSNTAAMIF
                     QDPLSALHPFYKVGSQIAEAYLAHNSVSKDIAAKRAVEMLELVGIPNPARRAKQYPHE
                     FSGGMRQRAMIALGLVNNPKLLIADEPTTALDVTVQAQILDLVKDLQREFGSAVILIT
                     HDLGVVAEVSDDILVMYAGRAVERGLAKEVLARPQHPYTHALLQSLPSASEDSDQLQT
                     IKGSPPSLVHLPSGCSFHPRCMVKDRVPGDPCATLLPELRVSSPGRQIRCHLTNPESV
                     LDQEVVS"
     gene            complement(36294..37280)
                     /locus_tag="PLB86_00510"
     CDS             complement(36294..37280)
                     /locus_tag="PLB86_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC63646.1"
                     /translation="MLIYIARRALMAVSVLLVVLVATFLLFFAGPSDPAQAMCGELRC
                     TPQRLEDIRRSLGTDRPVAEQFTEYFKGLAVGREIYVSGAPRTCSAPCLGFSFRTGIE
                     VKEALASRFPNTAMLALMTMAIFLTVGISTGVLAARRRGTALDRGVVGFSQVFGSIPS
                     YILGLLFALYTTVLYPILPRYANRSDGLGPWFVGLIAPAIILGLIYATGYVRYARASM
                     VDTLNQDYVRTARSKGISEQKVVYRHALRAALSPVVTILGLDMAGLLSGTLVTEHIFG
                     VDGIGKFAIESLRNDDLPIIMGTVLIGCVLVVVMNLIVDIAYSFLDPRVRLS"
     gene            complement(37381..39156)
                     /locus_tag="PLB86_00515"
     CDS             complement(37381..39156)
                     /locus_tag="PLB86_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC63647.1"
                     /translation="MRWTHVLAVGAVASLGLTAACSAPASTPGATTGATASQTAESKA
                     AAAIDPTAKGPAPEVAGAKKGGTLTVSYSTAPADMDPSAQFYQDSGAIMTRLTQRSLT
                     SFVNRNGKQVLVPDLATDLGQVSADGLTWTFTLKDGIKYSDGSAVVADDIAYAVKRSF
                     AFTDTGPTYQVDFLKDGAKYEGPWDNPETENPFGDTFAGVEAQGNKVIFHLEKRWETL
                     PYFATFTQVSPIPKAKETKNRDYGNTALSTGPYMIKSFTQGTELTLVRNPNWDAKTDP
                     ARTAYVDSYAFKFGQDSIKVQQGILASNGPDATTLNWDGIDASLADQVTGAKSSQFIN
                     GQSSCVVAVNMDSRKIPLEVRKAIAVAYPFDDINKAAGATPLSQTPASTLIPPQIPGW
                     LDYKLPGLTGTGNGDPVKAKEMLKAAGKEGFELVYYYTNDDPSNVAQQVNQVRKTKLQ
                     EAGFTVKDLGVPNKERRTLISKLDGPHNMLQSPGGWCFDWPSADSIFPPMVSSTQIKG
                     GGTNWGNLSDAKIDAEMERILKLSIADQGPEWGKFDKWLMETYLPAIPYYYDRSNLLF
                     GTKVKNVINDPNHGMPVLDAIWVEQ"
     gene            complement(39226..40227)
                     /locus_tag="PLB86_00520"
     CDS             complement(39226..40227)
                     /locus_tag="PLB86_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC63648.1"
                     /translation="MGSTTEVADDSAARATAAAATDRRSGRSPSQIAWDRLKRDKVAI
                     TSLVIVIFFILVAIFAPLLVALEGEELNKYHPELISALNTYPIIGANPQHWFGVEPRT
                     GRDLFAVWAFGARPSLIIGFVAATFSTILGIIMGLAAGYLGGKVDKVITWIIDLLLSL
                     PFLLMVLALVPIIQKRFAAPGEILTAEETSSIRFVVLIGILVFFGWIGLARLIRGEVL
                     SLREREFVQAAKVIGVPTRAILFREILPNLVGPIIVSASIAVPAYITAEATLSYLGAG
                     LVEPTPSWGRTVSNAQNAYARYPLFLWPPVLSIALLVLALSLLGEAIRDAFDPNTRR"
     gene            complement(40505..41908)
                     /locus_tag="PLB86_00525"
     CDS             complement(40505..41908)
                     /locus_tag="PLB86_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRC63649.1"
                     /translation="MFGAGAGTGADAAYPALDLPLAVRMRPRSLAEVRGQEAVLRVGS
                     PLRRLVEGSSGPAGPLSAILWGPPGTGKTTLAHLVAGASGRRFVELSAVTAGVKDVRE
                     VMAAATRERDLYGRHTVLFLDEIHRFTKAQQDALLPGVENRTVILVAATTENPSFAVV
                     APLLSRSVIVALVPLSDDDVAAVVGAALDDARGLDGAFTLDPDATAHLVRMAGGDARR
                     SLTALEAAAGVAGDQVPLGAAEPTRDTPTAITLAHVERAMAQAAVRYDRAGDQHYDVA
                     SALIKSMRGSDVDAALHYLARMLEAGEDPRFVARRIVIAASEDVGMADPTALQTAVAA
                     MHAVAQIGMPEARIVLAQAVVHVTLAPKSNAAYLGIDAAIADVRAGRGGPVPLHLRGS
                     GYAGSARVAASAAAAGAGPADAVYRYAHDAPEGVATQQYLPDDLSGRVDYYRPTGRGF
                     EERLATRWTWLKGLLRG"
     gene            42119..44245
                     /locus_tag="PLB86_00530"
     CDS             42119..44245
                     /locus_tag="PLB86_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRC63650.1"
                     /translation="MVLTWAVLAVAAFLVAAAGVGGPSLFSKLSTGEPTAPGEATTGR
                     DLLAAAEPDRPAILLTVDGVDLVSPAVATAAAAATADIAAIDGVADVASPFEFPAGTA
                     DPRAAAFVTAGSTPPKAFLMVTRLEAGLEGAAQDRAEVAVRAAYTRMGGTLPGASIAI
                     GSGAALGDEIVGQMEADLRLGEGIALPLSFALMVVVFGGFVAAGMPIIGAVVSIGGAL
                     AALYGFSTVMDLDAATVNVVTLLGLALSIDYGLLFVSRAREELRALAEGRPAADLTHE
                     QIRLAMARTTATAGRTVLFSGVTVAISLAGLMVFRSPTIRAIGAAGMSVVLVAMLVGV
                     SMMPALCTLGARRLLARGTETAPDEGVFSRLAAAVHRRPWVVMIATSALLLAMAVPAL
                     SMRLTSSGVELVPVGSPQRDFYERLDRDFPALRAPTATVVVNGSLQQAQDWAQGTVAR
                     LPGVTQAQARDLGDGYAAVAVHTGAAPLDEGSRALVETLRANRPAVPIWVTGQAAALL
                     DLTGSIAAGAPYAGLLVVFATFVLLFLMTGSVVIPVKALALNVVSLGASLGALTWIFQ
                     DGHLASALGFTSVGAIESIIPPLVIAFGFGLSMDYELFLISRIAELHQAGVENNRAVE
                     LGLQRSGRIVTSAAALVVIVFAGFIAGKMLIIKEMGVALTVAVLLDATLVRMLLVPAT
                     MELLGERNWWAPAPLARWHARHGIQE"
     gene            complement(44249..46030)
                     /gene="aspS"
                     /locus_tag="PLB86_00535"
     CDS             complement(44249..46030)
                     /gene="aspS"
                     /locus_tag="PLB86_00535"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRC63651.1"
                     /translation="MLRTHEAGTLRADHSGQTVTLAGWVAKRRDHGGVAFLDLRDASG
                     VVQVVARDEVLDDGGGHDLRNEYCVQITGEVRLRPEGNANADLPTGAVEVVASTLEVL
                     NAAAPLPFQIDERVTVGEEARLKYRYLDLRRPGPGAAIRLRSKVNAAAREVLAGHDFV
                     EIETPTMTRSTPEGARDFIVPARLAPGSWYALPQSPQLFKQLLMVAGMERYYQIARCY
                     RDEDFRADRQPEFTQLDIEMSFVEQDDILALGEEIAVALWKLIGVDIPTPLPRLTYAE
                     AMARFGTDKPDTRFGQELVDCTAYFADTAFRVFQADYVGAVVMPGGASQPRKTLDAWQ
                     EFAKQRGARGLAYVLVQEDGELGGPVAKNLSDAERAGLAAHVGAAPGDCVFFAAGVTK
                     ASRALLGAVRLEIAKRGGLIAEGSWSHLWVLDAPLFEPAGDAVAAGDVAVGSGAWTAV
                     HHAFTSPKREFIDTFDSDPGSALAYAYDMVCNGNEIGGGSIRIHRRDIQERVFAVMGL
                     SEEEAREKFHFLLDAFAFGAPPHGGIAFGWDRICALLSGAESIRDVIAFPKSGGGYDP
                     LTGAPAPITPQQRKEAGVDSKPVPPTA"
     gene            46253..46990
                     /locus_tag="PLB86_00540"
     CDS             46253..46990
                     /locus_tag="PLB86_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63652.1"
                     /translation="MRTGVQGLRGWRRAAPVTVAAVGLLLTAGACPAPPIESDAVDRA
                     RVDALLAADPGLASTEVSYGGGGVGSRNLRYLRPSITVRGTLVPAHPAGSLEGLTAKQ
                     VLAADEGSAVAALRSTLEDLRRRGWVVTSANCEVITSVGTSLHRSWLVEARRPMASDP
                     KVWALLQLSAAVSRNYGSADLSFPRVEMIQQARLPFHLDEPNVFAPPSGLPAGGLTST
                     CADQATLPSANASEGSAFPPLVDGPPR"
     gene            complement(47006..48610)
                     /locus_tag="PLB86_00545"
     CDS             complement(47006..48610)
                     /locus_tag="PLB86_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63653.1"
                     /translation="MSGEWQVTHLLNESRSFLRTLGHVAIALVWPPSLIADIERFRRT
                     ISYPPPGDPATIEGMATSLDTAGRAVDEVRADLDRRRVGLPAIVVGVAAGTATQAAAA
                     LSSGLSHEVTGLALSARALRRYAASLQDAITEHGRVDDHFDDAVTHLRRCADLTVEVH
                     GVDTHIPDPVAVTREVSRALADAREGVRTAVRAYEQAETALRALQREGSDGRGWAVLA
                     SAPRVPGFTVLDVAVMVDQWRTSDPDNRLLNPDQWQRVGAMLAALTPAQRAALDELLA
                     AAGSSTQRAVLLSTLAAGADMEQLRFMADAVRSMSDEDIARMMSLNTDNLTSGATYGL
                     DRVIQDPTTGNTCASTSLLALLARSDPMLALWLTSGQRPPGWDPAYLQHLTEAQWNGA
                     TFLERFIAAQHSVLTTTDSDLGTWPEALGTPPWGAAALATLGSPTAYESVLYDDQDPA
                     LVRKLMSRAAEAANNGTPVPFYVGDDYTPRHVMLITGYADGYYTVYEPGSGSITRVPE
                     SVMLGTGTPGAFGGWSHIDYLVLPVT"
     gene            complement(48607..48924)
                     /locus_tag="PLB86_00550"
     CDS             complement(48607..48924)
                     /locus_tag="PLB86_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63654.1"
                     /translation="MTTSTDEALHLARVGDALVDAHVDVTALRQIAWAAELAGLRAVL
                     ALGPGLLTDPRAGAVLVDALQPLAVDLADGQPQSPDLTHRVRRFFADLVMVVGALTHE
                     DPA"
     gene            complement(48921..49178)
                     /locus_tag="PLB86_00555"
     CDS             complement(48921..49178)
                     /locus_tag="PLB86_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63655.1"
                     /translation="MTWDEGGAGEASGQVASMADAVQAQSRRLAGITVNQGDATGMIS
                     ELLHTWATELDLRGEALDVWATAVRAQTETVARTDHRMRLA"
     assembly_gap    49211..49310
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(49516..50205)
                     /locus_tag="PLB86_00560"
     CDS             complement(49516..50205)
                     /locus_tag="PLB86_00560"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRC63656.1"
                     /translation="MAFDAPIGTAALLRDEIAPKPVVASDVVWQGLVFDVRRDRVDLG
                     AEVVTRDYADHPGAVLILALREIDGVDHIVMIRQYRHACGSYLWELPAGLLDIPGEPP
                     RDAAERELREEVDLVAGRWDLLIDAYASAGVFPEAYRVFLARDLAPAPGEPHVRTGEE
                     ADLTVRWVSLDDAYAAALAGQLHNPGALLGILAAWGARAQGWSTLRPDDAPWPEHPAY
                     RDGHRTGPQAG"
     gene            complement(50207..>51556)
                     /locus_tag="PLB86_00565"
     CDS             complement(50207..>51556)
                     /locus_tag="PLB86_00565"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRC63657.1"
                     /translation="IPHITNEIKARMRALATGDKDAPDLIITEIGGTVGDIESLPFLE
                     AARQVRQEIGRDNCFFLHVSLVPYLAPSGELKTKPTQHSVAALRQVGIQPDGLVLRAD
                     REIPEPIKRKISLMCDVDQDAVAAAVDAPSIYDIPKVLHGEGLDAYVIRRLGLGFRDV
                     NWTSWNEMLKRVHDPEHQIEIALVGKYIDLPDAYLSVTEALRAGGFHHDAKVVIRWVA
                     SDECQTEAGAQRALGGVDAILVPGGFGVRGIEGKLGALRWARERQVPTLGICLGLQCM
                     VIEYARTVLGIADASSSEFDPQTTHPVVATMAEQLAFVEGAGDLGGTMRLGRYAARLT
                     PESVVATAYGTTEVSERHRHRYEVNNSYRERLEGAGLVFSGLSPDGSLVEFVELPADV
                     HPYFVATQAHPEFLSRPNRAHPLFAGLVGAALDRQRAQRLVEVERTTRPESQTAAVG"
BASE COUNT         7731 a        17296 c        18301 g         8028 t
ORIGIN      
        1 cggcgaccca gatgcgcgag gactcgtcgt cctcgtcgtt ccagccggtg ttgcccacgt
       61 gcggtgccgt catgacgacg acctgccggt ggtaggaggg gtcggtgagg gtttcctggt
      121 agccggtcat ccccgtggag aagaccgcct ccccgagggt ctcccccacg gcgccgaagg
      181 actcaccgag gaacgtgcga ccgtcctcaa ggaccagcgc tgcgggctcc cgatgcctgc
      241 gcagcgacgt tgcgggactc acgcgatctc ctccgggtcg aactggccgc gaacgcgacc
      301 tcggtatgcc gtgcactcgc cgcgcaggaa cgtcgcgacg accggggcgg gcagggtgcg
      361 cccgtgccag gggttgttgc gggacagcga ctggctggtg gaccggtcga cggtccacga
      421 cgcgtcgggg tcgacgagca cgaggttggc cggctcgccc acggcgatcg gccggccctg
      481 atcggccagc ccggcgatcc tcgcgggggc gaaggacatc cggtcggcca cgtcggccca
      541 gctcagcagc cccgtccgga cggccacctc ggcgacgacg gcgagcgccg tctccagacc
      601 gagcatcccg aacgccgcat ccgggaacgc gtgctccttg tcgtgccggg cgtgcggggc
      661 atggtcggtg gcgacggcat cgacgacacc gtcgacgagc gccgccagga tggccgctcg
      721 gtcctcgtcg ggtcgcagcg gcgggttgac cttgaagacc gggtcatagc cggtcagcag
      781 gtcagtgccc agcgccagat ggtggggagt cacctcggcg gtgacggcga tcccctgggc
      841 cttggcccag cggatgatct cgaccgacgc cgccgtcgac acgtgcgcga catggacccg
      901 cgagccggta tgccgcgcca gctggacatc gcgggcgacg acgacgtcct ccgcgatcgc
      961 gggccaggcc ggcagcccga gccgaccgga gacctcgccc tcatggcagc aggccgaccc
     1021 gtcggcgagc cgagggtcct gggcgtgctg ggagatggtg cctccaaacg ccttgacgta
     1081 gtccagcgcc cgacgcatga gacgggcgtc gtggacgcac ttgccgtcgt cggagaacac
     1141 ccggacccgg gcacgggagc gggccatcaa cccgagctcg gcgagctcga cgccagccag
     1201 gcccttggag acggcgccga tcggctgcac gtcgaccagc ccggccgccc ggccgaggtc
     1261 gaacacccgc tcggccgcct cagccgtgtc ggtcaccgga gaggtgttgg ccatcgcaag
     1321 gatcgcggtg aaaccacccg cggccgcagc ggccgagccg ctcgcgacgg tctcggcgtc
     1381 ctcgcgaccg ggctcacgaa ggtgggcatg gaggtcgacc aggccgggca gcaggacgag
     1441 cccggcagcg tcgacaacgg tgagccgccg tccgtcgagt tcgcctgcag cggggataga
     1501 cccgatttcg gcgatgacac cctcgcggac gaggacgtca gcgccgttgt ctcccaacag
     1561 gtttgcgccc ttgagcagga ggtcggtcat gcggcagcct ccgtctcgcc ggccaggagg
     1621 tgatagagga ccgccatccg cacggccact ccggcagaca cctggtcgag gatcgccgag
     1681 ctcgtcgcat cggcggcttc ggccgagatc tccaggcccc ggttcatcgg cccggggtgc
     1741 aggatcggca ggtcgggggt agcggcatac agtcgggcca agcgctccct cgtcaggccg
     1801 tagcccgcgg tgtattcgcg ttcggtcggg aagaacccgc cgctcatccg ctcgcgctgg
     1861 acccgcagca tcatcaccgc gtcggggcgg tcggcgggcg acgcggcata cagggtctgg
     1921 tcgaggtccc agctcgtcgc gaaaccgtcg cgggccgccc agtcgccgat cccgctgggc
     1981 atcagcgtcg gcggggcgac gacggtgacg cgcgcgccga gccggccaag ggtgatgacg
     2041 ttggagcgga acacccgcga atgggccagg tcgccgacga tgacgatgtg tcgaccctcc
     2101 agcgacccga ggcgttggcg cagcgtgtag gcgtctagga gcgcctgggt ggggtgctcg
     2161 tgggtgccgt caccggcgtt gatgaccgac gcgtcgaccc actgggcgac ctggtgacag
     2221 gcccctgagg cactgtgccg gatgacgaga gcgtcgaccg acatcgcgca gatcgtcatg
     2281 accgtgtcgc gcagtgactc gcccttggac gtcgaggagc ccttgccggt gatgttgatg
     2341 gtgtcggccg acatccactt gccggcgatc tcgaacgacg accgggtgcg ggtggagtcc
     2401 tcgaagaaca ggttgatgac cgtgcgccca cgcagggtcg ggagcttctt gacctcccgg
     2461 cgttgcacgt cgtgcatcga ggcggcggtg tcgaggatcg agtcgacctc gtcgcgggac
     2521 aggtcggcgg aggacaacag gtgcttgcgg gtcatcggtc gcctccgctg atgcgcactt
     2581 cgtcgtcgcc gccgtcgttc tcggccagca cgacgtggac gcgttcggaa ctgctggtgg
     2641 ggaggttctt gccgacgtgg tcggcgcgga tgggcagctc gcggtgccca cggtcgacga
     2701 ggacggccaa gcgcactgcg cgtgggcggc cgagctcggt cagcgcctcg agggcggccc
     2761 ggatcgtccg cccggagtag agcacgtcgt cgacgagaac gacgatcttc ccgtcgaccc
     2821 ctgacggcgg gatcttcgtc ggggttacgg tgcgggtggg ctggcggcgc agatcgtcgc
     2881 ggtgcatggt cacatcgagc gagccgacgg ggaccgcgac tccctcgacc tcggcgatga
     2941 cgctcgcgag gcggtgcgcc agcggcacgc cacgggacgg gatgcccagc agcacgaggt
     3001 cggccggacc cttgttgcgt tcgaggatct cgtgggccac tcggcgcaag gcccgggaaa
     3061 cgtcaccgga gctcatgacg acacgtgcgc ctgggggtgt cggcgagggg gtctgatcgg
     3121 acgccaccac gcggacctcc ttccccgcct cacaggacgg tggttaaagg atgtctggtt
     3181 cgcgggtaac cctactctcg gcgtcgggtc gcctcgcgcc gggtccgtat gccggtcgcc
     3241 tccgccgctt ccagccgggc cccgggaggt gccctcacaa ctcgtgcgaa gcggggggtc
     3301 gcgcgtcaca cttgacgcat gagttccgac actcccgcca gcgaataccg catccgggcc
     3361 acggccgagg acgtgcgccg gatgatgtcc cgcctgctcc tcaccgaggg cagcgacacg
     3421 agaaggaaga tgtcagcgtt ctgggtgctc ttggtcttgg ccaccgtcat cgccacgtcg
     3481 ggtgtcgtgg ccgattccac ggcgaccgtg atcggcgcga tgatcgtcgc tcccctgatg
     3541 acgcccatcc tcggcaccgc cttgtcggtc gtgctcgcca accggcgtct tgttgcggtc
     3601 aacttcgggc tggtgatcgt gggggccttg tgcgtcatcg ccatcgccta tctcatcggg
     3661 atgacgtcgc agatgcacct cgtcgctgac agcaactcgc agatcgccgg gcgggtcagc
     3721 ccgcggctca tcgacctcgt cggggccctg gcgaccggcg ccgtgggggc gttcgcgacg
     3781 gtgcgctccg acgtgtccga cacgttgccc ggcgtggcga tcgccatctc actcgtgccc
     3841 ccgttggccg ttgtgggcct caccctcgag tcgggcgagc cgtcccaggc gcttggtgcg
     3901 ctgctgctct tcggcaccaa cgtcagcgcg atcatcgcca ccggcaccgt ggtgttcctc
     3961 agctaccgcg tgcgtcaggc ggcgatcgat gcgggtctgc ccatcgggcg gctgcaccga
     4021 ggcgcgctcg tcaccgcctt cgcgtcggtc gtcatcctcg ccatccccct gtccctcggc
     4081 agctcggttg tgctccagca ggaggtgctc gtctccaagg cgaccccggt cgcccgggcc
     4141 tgggccaagg ctcacggcgg ccagatcagt gatgtcacct accgcgacaa ctccctgcgg
     4201 atcctcgcca tcggcctgct cacgctcgat caggtcaccg gcctgcgtcc ggacctcgac
     4261 gccgccgggc tgggcgatgt cgacgtgctg gtgaccctcg tcgccggcgg aacgaaggaa
     4321 ctgcccggca agccgtagcg gggtaggtca ggtcgtgttg cgatatggcg ttgcgcgctc
     4381 accagggaca ggccggggac gaaccaacta gggtcgcact cggaaggagg cggcaacgga
     4441 tgcaggctcg ccaggtgcgt cccctggacg cggcatacct cgcgctcgac ggaccccgca
     4501 cggtcggcca cgtctgcctc gtcctgcccc tcgatgggcc gatcacgctg acccagttgc
     4561 gcgagcaggt cggcgagcgg gtcgggcggc tgccggagct ccgccagcgc ctgcaccagg
     4621 ggcccaacgc cctcgaccgg ccctggtggg tcgacgaccc gaccttcgac atcgaggccc
     4681 acgtcgagga gttccacgtc gggcggcgcg agctcagcga gttggtgtcc gagttggcca
     4741 tggaggcgct gccgcgcacc cagccgctct ggcaggtgca cctcctgcgc gaaggccgcc
     4801 gctgctacgt catgacgaag gtccaccaca gcatcgccga cgggtctcgc taccgcgaca
     4861 tcctccacgc gctgttcggg accgcgcagg acgacgtccc cccgtggtca ccccagcgca
     4921 tccccagcgc cttcgacctg gccacccgcg ggctcgtcgg ggcctcgcgc tgggcctgga
     4981 cctcggtgta tgccgccgcc cgcgccattg ccgacaaccc ccagtcgctg ggcggcgtcc
     5041 tcaactcggt cggcgtccac ggcgcgccgc cgaccccgtt caaccgttcc gtgggaccca
     5101 accgggtctg ggcgttccgc acgtgggggc tggcggcgag caagcccacg cgggcgcact
     5161 tcggggtcac ggtcaacgac gtcatgcatg ccgtggtggc tgccggcctg cggcagtggc
     5221 tcatcgagca gaacgcgctg ccgaccagcc ctctcgtggc gctcgtgccg atgtcggtgc
     5281 gacatctgac gaccgacccc tcgggggcca accggctctc gcttggcctg tgccggatcc
     5341 cgaccgacat cgaggacccg gtcgagcgga tcttctcggc ccgcgaggac atggccgaag
     5401 ccaaggagcg accagtcctc ggcgaaggag cccttgaagt cctcacccgg ttgttgggcc
     5461 cgaccttgga gccggcgagt tgggtcgcgt cgaccgtgcg gctgctcgac accgtccggc
     5521 tgccgttcaa cacgatcatc tcgaacgtcc ccatggggca ggacctgttc gtcatcgccg
     5581 gtcgccgggt gacggccgtc tacccgttcg cgccgttggg cgacggcatg ggggtgagca
     5641 tcaccatcca gggctatcag ggtcggctgg acgtcgggat cagcgcatgc gccgatctcc
     5701 tgcccgatgt cgagcacctc atggacctga tgacggccga atacgcccac gtctgcacgc
     5761 tcgcgtccct caccggcggc tgaactccgg ccgctgccgt ccctacccgg ccgtcggtgg
     5821 tgacggcggc ggaggtggcg gagcagcacc ccagttgggc gggggcggcg gaggcggagc
     5881 agcaccccag ttgggcggtg gtggcggagg cggcggggcg gcaccccagc ctggtggtgg
     5941 cggaggctgc gcagcgccac cgggagaagg aggaggcggc gcggcggcat acccctgcgg
     6001 agcttgggca gccgcgccgg gcggaggggg cggcggcggc gcggcagtct gcagcgcgaa
     6061 cgagggcagg gcgccctggg cctcaaagcc ctgcaacgcg gtcatgatcc ggtctcgctc
     6121 ccccgcgtcg gacggccgac ccgtggcgtc gaggtaggcc agcaccccga ggtcgcggaa
     6181 gaacttgtcc gcctgtttcc cacccaccga cgggccggcc agaccctggc ttgccagagc
     6241 ctggtcggcc ttcgcactca actccgtcgc cgcagccttg gccttgtcca ggaaccccat
     6301 acgacaccgt cctctcgaaa ctggatccga tgggtcccca tcgtgctgca ccacgggggc
     6361 cagcgcagca gaaacgacga caagccggtc gagcgacagg tatggtctgg cccgataccc
     6421 cacccgatcg acacgaagga ctcgtcgctg ccatggctat tcacggatcg ctgttcacgc
     6481 agtaccagga gaacccgtca gccgatccgt tcgtgctgca gaacaagaag ctgctcaaga
     6541 tctcgatgca gtacggcccc gtcttcgcca aactcggttc gatggtcgcc tatcaggggg
     6601 acgtgcggtt cgagaacccc ggctcgggcg ggctgaacaa gatgttcaag tcagccatga
     6661 ccggcgaggg cgtcaagatg atgcgctgca ccggcgccgg tgagttgttc gtcgccgacg
     6721 cagcggccga ggtccaggtc ttctacctcg agaacgacat ggtgtcggtc aacggcaaca
     6781 acgtgctggc cttctcgtcc tcgatcgagt gggacatcca ccggatccaa acccgcggca
     6841 tgatgaccgg cggtctctat aacgtgtcgc tgcgtggcac cggctacgtc gccatcacca
     6901 ctaagggcga ccccgtggcc ctcgacgtcg ccggggcgcc gaccttcggc gatgcccagg
     6961 ccgtcgtcat gtggacttcc ggtgtctcga tggacatccg ggtcgacacc ggcgggctca
     7021 gctcgatgat ccgcggtggc accggcgaaa ccttccagat ggccttcggc ggccaggggt
     7081 acgtcctcat ccagcctgac gagggtgtcg tcagcggggg tcaccaggcg tagcaccgcg
     7141 ccgggagacg gacgatggct ccggtcagcc gagggtgtgt ttgacctcgg cgatccgggc
     7201 cagcaggccg ttgacgaagg ccggcgattc gtcggtcgac aggctcttga ccaactcgac
     7261 cgcctcgtcg atggcgaccg cgtcagggac ctcgtcgctg taaacgatct cccaggtcgc
     7321 caggcgcaag gctgcgcggt cgacggccgg catccgctcg acgctccacc cttggctata
     7381 ggtcgcgagc atctcgttga tctctgacca acaccgcacg actccctcga cgagggtgat
     7441 cgtgtagtcc gccaacggcg ccggagtcgc cggacgcgcc agccgctcga tgagcagttg
     7501 ctgcgcgttg atcccgcgtt ggtcggcctc gaaaaggatg tccagcgcgc gtttgcgtga
     7561 cttcgtgcgg gcgcccacca gcgtcagtcg ttgacgcggc cgaggtagga gccgtcgcgg
     7621 gtgtcgacct tgacccgggt gccgctctcg aggaagagcg ggacctggat ctgggcacca
     7681 gtctcgagcg tggccggctt ggtgccgccg gtcgagcggt caccctgcaa gcccggctcg
     7741 gtgaaggtga tctccaggac caccgacgcg ggcaactcga catagagcgg caccccgtcg
     7801 tgcgtagcga cgatggcgtt ctggttctcc agcaggtagt cggcgccgcc ggcgacgacc
     7861 gcctcagaca cgtgcagctg gtcgtaggtg tcggtgtcca tgaagacgaa gtccgacccg
     7921 tccttgtaga ggtactgcat cgtgcgcttg tcgacgttgg ccgtctcgac cttgacgccc
     7981 gcgttgaacg tgcggtcgac gaccttgccg gacaggacgt tcttgagctt ggtccgcacg
     8041 aacgcggggc ccttgccggg cttgacgtgc tggaactcga tgacggccca caactgcccg
     8101 tcgatgttca gcacgaggcc gttcttgagg tcgttcgttg ttgccacggg tggtcgcttc
     8161 cttgctcgcg ggtatgccgg gcgcgccagc gcccgtcgaa gatctggtgg gattcgggtc
     8221 cagtctaccg gaaaaccgtt tgccgcctcg cgctcgcgcc tggcaccctc gacacgtcgg
     8281 ccgagggagc agccggggcg gcataccggt tcccggtcct gcgcacgacc tggccgacac
     8341 aggtcttggg aacctgctag tcggctcacg ccaccagggg tgcgactccc ctttctggtg
     8401 acgccgggcg gatcacccga cccaacggcc ctgcggccca cccccgcgct gccccggcag
     8461 tgcggtgggt gtcgccccgc ccgacggtcg ccgcccggcg ccactccctg ccccttgccc
     8521 gtgccccttg cccctacccc acgcccctac ccaacgatgc accaaggagg tgcccgtcat
     8581 gttccgaatc acgatcccga cccgtcacgc cgcggtcgtc taccacgccg gccggttggt
     8641 cgacgtcctg cccgccgggc gcacccggcg tcggtggggg caccactacg tgctggtcga
     8701 cctccgggaa cgccagcagg tcatcccgcc ccaggacatc ccgaccgccg acggtctggg
     8761 cgttcgcgtc tcggcggtcg cgcgttgggc gatcaccgac ccggtcgcct tcctcgaggt
     8821 ctcccagacg ccggccgaca ccctccacct ggcgacccag ttggcgctgc gcgaggcggt
     8881 gggcgcgctc gccgcggctg acctggtggt cgcgctgcgg cggctggacg tcacggcgcc
     8941 ggtcgacgcg gcaactcgtc aggtcggcat cgcgacgctc ggcgtggcgg tgcgcgacgt
     9001 cgtcctgccg cacgacctgc ggatcgcgac cgccgaggtg gtcacggccc gtctgcgggg
     9061 cgcagccgcg cttgaggcgg cccgcgccga gaccgcggcc ctgcgggcgt tggccaacgg
     9121 ggcccggctg ctcgacgagc acccggcgct ggctcagttg cgcctcgtcc agtccgcgcc
     9181 gcccggcact cgcctggtgc tgcagttggg cgggacgccg gagccagccg gcgactgacc
     9241 acgcccgacg ctcgcccgtc gtgggcgatt ggcggcctgc cgggtatgcc gcgcgcacgc
     9301 agtgtgcacg cggcataccc ggccctggcc ggggtcagga gacgaaggag agggccttcg
     9361 cgggcgagcg ggtgtccatg ttgcgcccgc ggttctggtg gtcgtcgatc tcggcggcac
     9421 acccgatcat ccggccgaag aggaacaccg tgaaggcggc gttctccaac tgaccttcgg
     9481 tgaacgaccc gtcccggtag cggctccaga gcatcttgag caggcccgtc gcgatgagcg
     9541 catcgacgtt gacgcagaac acgttggtgg ttgcgccggc gtcgcgcagc gcgacgacga
     9601 ggcgacggta gtagtcgtgg aagacgttgt agtcaccgcg ctgcgccatg accgtgcgga
     9661 cgaactcttc gcgcgggtcg acgttgacgt ccttgccctt gaagaccggg tggttgatgc
     9721 acggaatggt cgcgcgatcg gcacccaact ccttgcgggt cgtcttcaac tcggcgaagg
     9781 cgacggcata ctccttcgcg agggcgtcga ggtcgacgcc gtgagcgggg tcgcccgggt
     9841 cgacgaggcc ggtgcccgcg aaccgctcca gtaggaacgc gatcccctcg aagccgttgc
     9901 ccccgtgggc gtagccggca tgcgtgagga aaccgatcat cgccttgttg agctggatcc
     9961 gctcgggggt ctgtgggccg tcggccgaga ccgcaccctt ggccccctgg gcggtgatgg
    10021 caccggggcc gttggtgagc agcagtccgg tgagcatcgc gaagccgtcc aagatgtcgg
    10081 cggagggacg ctcgcccagc agggctgcgg cagcgagctc cgtcaccgac gcctgaccga
    10141 gaacctgcgc catgggtatg ccgcagaaac cctcggcggt gtgcgccgcg gtcggtgcgg
    10201 cggcgccgat catcgtgccg aacagctcca accaccaggg cagttcctct gccgtgatcc
    10261 gagagatctc cttgtgcgcc aaggacttcc agacgagagt cccagcgatg gcggccacga
    10321 caccggggac ctggggatgc ccgccctggg tcgccaacca gcgcagcggc atcgaggtca
    10381 cgccgcgttc ggccaggcct gcgagcatcg cctcggcgat cgggtcggga tcggcgccga
    10441 tcgccagggc gcgcaactcg ggcggcgccg ggtatgccgc gaggtcggtc tccgtgtcgt
    10501 cgaccgacag cccgctgccg gtgaagaggt cgatgaagcc atggatggca tgtcgcgagg
    10561 cctcggcgcg gcccggcccg gcgatggcga gcgcggcggc gatgaccgtg ttgggggtgt
    10621 taccggcagc ccgggccgcg tcgacggctg cgagggtgac ggtgccggcg aggttgacgt
    10681 gggcgccaac cgccacgtcg acgagcgccc ggtcgcgctc gctcgcgcga cggccgagca
    10741 gggccaggac gaggttgtcc tccatcgggt gctgggccgc ttggaggatc gacatgcccc
    10801 gcaggctgga cacctgggtc agcgggtcca tctgggagac gcctgacgcg tccttcatcg
    10861 actgccgcgg caggacggcg ccaatctgcc ggttgagctc ggcgatctgc tggtcgtagg
    10921 gcggcatggc cacgacgttg ggcaggtcga gctcgggcgg aagctcgagg tcgcgggctc
    10981 gaccgaacca tggcttgagg gcgaggctcc cctcgggggc gaaatcgggc atggtcgcgt
    11041 tgagggccat cactgcggtc agggctgccg ggatgtgggc gatgttcgtc acgaccgctc
    11101 cgcgacggga gcagaccgga ttgtcggggg tgaaggcgtc ctcgaccccg aacttctcca
    11161 tgaaccagcg ttccttggtg gcagcgtcgt cgccgccccc ggccatcgca cccgcatggc
    11221 cgacggcgcg ggtcaggcga gacttccacc gtccgacgac acaggcgacg accggcttgg
    11281 tgaactcggc gtcccactcg tagtagccgc ccggctcgac atagaggacg gccgccttgc
    11341 tgcgcatgtc gttggccaac gcgaacgcga actccggtgc ggcgtaatgg atgtagacgt
    11401 ccttgccgct ggagatgagc gtggtcgtgc cccagccggc catccgcagg taggtggcaa
    11461 tcgtcgtcgt gaagttgccc gagttggaca gcaaggcgat cgatcccttg cgcagcgcct
    11521 cctcaggggt gttgccgccc agggcgccgc cgatgcgcac gtggttccac gagtcggcca
    11581 cgccgaggct gttggcgccg aagatgtcga ccccgttgga ctgggccatc gcccggatct
    11641 cgcgcgcgtc gtgagcgggg atcttctcgg tgacgatgaa gatcttgcgc atctcggggt
    11701 tgacccggat gagttcaccg accccgtgcc gggccgcagc ggggggcagg tagacgaccc
    11761 cagcattgaa ccggtgcccg tcggccaggc cttccaggac gttgttgtag acggggatgt
    11821 cgcccaggtt ggtcggcatg tgcgacccgc ccttgccggg cgaggtgccg aagacgacgt
    11881 tgcccccgga gtactcatgg ctcaccgggg tgacgtcgct cgactcacct ccgaggatgt
    11941 tgagcacaca cacccggtcg tccttggtgg cgatctgggc cagtgagctg atgccgacgt
    12001 agaacttgaa ctcgccgacg cccttcttgt acatctgcag gtctccttcg tcgccgcgtc
    12061 agccggccaa agcctgctgt tggcgggcga tctgctcgcg ccccgaggcc ttcatccagg
    12121 cgtcagcggc gcgggcgtag ttgatgacct cggacatgtc tgagtcgaag ccgaacatct
    12181 tgtaggggat cttgaggctc tcgaaggtgt cccgcaggac gcccatcccc cggatgaggt
    12241 tcggcccgcc gcgtccgatc acggcataca ggggcgttgg cccgtgcgtc gcgtagtgct
    12301 cgcgcagggc gtcggccatg gcccggaagg tctcgaagat gtcggtgttg ttggccttgc
    12361 cgccgatgat gaacagcacg ttggtctgcg gcagccagta cttgtaacag atccgcgcga
    12421 gttccttcat cttgacgtag ggcgggttgc cgccgaagtc cgaggagatg atcgcgtcgt
    12481 cgccgagcat ctcggacacg agcgagttgg cgccgccgcc gaaggtcggg gcgaggatcg
    12541 tccccttctc gttgacgacg tagacgtcgc tctggccttg gtaggtgcgc agttggttga
    12601 cctcgtgctc gaagtcggag tagctcgagg agaacagcga gcgcggcagg tcgaggcggg
    12661 ccacccgggg gtcgtcgcgg tcgaaggcgc acttgaaatc gcaggccacc ggcgtgagcc
    12721 ggccgcgctt gtccgcgcgc atccggatcg ggttgagctc gagggtggtc atcccgaact
    12781 cgtggaagag ctcccacagg ctgggcaggt gctgcacgag cggcgaaacg atctcgctgg
    12841 gtgcctgcaa ctgcgacagg gcgttcgaga cgacaaaggc gcgcatgccg gtgagtgctt
    12901 cgaagggcac cgacgcgacg agggacttgt ccaactcctc gatggcgacg ccgccgtggt
    12961 gggtgatcgt catcgtgggg gcccggaagt gggtcgagtc ggtgagggag aagtagacct
    13021 cgtgctccgc cgggatcatc gcctcgtagg tcaccccgtt ggccttggcg agcgtcgtgc
    13081 cgacctgatg ctcggcgaag tagagccgtt ccttctcgcg cagcgcggtc ggcaggtcct
    13141 tggcccggcc gatcaggccg gccttgccct tcttgccgac gccgcccttg aagaccggtt
    13201 tgatgaggat ctcaccgtgc cgggagatga gatccttgat ctcttcctcg gttgctgccg
    13261 ggcccaacac ctccgccgcg gggaagcccg cgaactgaag aagtttcgct ccatagagca
    13321 ttccggtgag attcatcctg ttactccagc gggtcgccgt tgatcctttg acacggtaag
    13381 ccttccggcc cgacccaccc cgggacgcta gtcccgtgag tcaggcgtgg tgagccaggt
    13441 gagcaccatt tcggtcacga gagcgatctg gcgttccttg tgctcgtgag tgcccatccg
    13501 caacccgaag gtcgcctcaa tggtgggtgc gttggtgatg cggaacagcg ccatggacgt
    13561 catcatgagg tgcaggtcca gcgggtccac gtcgcgcacg atgctgccct cggagcgacc
    13621 gcgcgccaag aggtccgcga ccaacgcgat gatcggcagg ttgaccccgg ccgctcgctg
    13681 ggacagtcgc aggtgcgagg cgtccagggc gttctcggag gccaccagac gggccagccc
    13741 cggatgggcc tcgtgatagt cgaaggtatg ccggatgagc acggtgagag cgtccaaggg
    13801 cggcatgtgg gccaggtcca gggcctgctc ggtctcccgg atatcggcgt aggccttctc
    13861 cagaactgcg gtgaacagcc cctccttgtc accgaagtag tagtagatca tccgcttggt
    13921 cgtggccgat ttggcggcga tctcgtcgac gcgagccccg aagtagccga tccgcgcgaa
    13981 ttcctcggta gcgacggtca gcaggtcggc ctgggtccgg gcggcatcgc gctgcttgcg
    14041 ggcggccttg ggggaattcg acacggatcc aactgtaggc cggaccccag gtgtcggagg
    14101 cgagccgacg gggcgcgcag agctggccgg ggcaggcggc ggcataccgg cctcagcgcc
    14161 gccgtgctcg cagcatgagc agcccgttgc cgacggtgct ggccactccg acgcccaaca
    14221 gcagccaacc gaacgggccc atggaggcgc cgccgtcacg ggcgacgtac cacccgagcg
    14281 ggatgagcag gaccccgacg acgagaccga cgatggggtt ggtggttgag ctcatagtgg
    14341 gctccaggtg tgggggtggg gcccaccggg cgggacgtgg gagaacgtcc cgcccggcgc
    14401 acgggccaag ggcacaacgg tatgccgctc acgcggcggt ccgctgatta agcggcgacc
    14461 ttctgggcct tcgccgagtc gatcgcagcc tgacgcagcc ggaggtactc cgcctcggga
    14521 accggcttgg caccggggac gccgaggtcc tcactgcgga agcgggaggt ctccggcgag
    14581 aagtaggcac cgaagaagga gatgatgcag atacccatcg tgatggctcc gacgacaagc
    14641 cagacgttct gcgtgccggg cggtgccacc caggcgagga cgaacgccat cgccgaagag
    14701 atcgcggtgc ccatgttgtg ggcgatcgcc attccggtca cccgggtgcg agtggggaac
    14761 tgctccgggt agaaggccgg gtagacggcg ttctggccct ggtagaccgt gccccacata
    14821 agcagcgaga aggcaatggc cagcgggacg ttcttctggc tcaccgacca gaggtagccg
    14881 aaggacagca gcccacaggt gatcgtcgcg aacatgatga gcggtcgacg accgatcttg
    14941 tccgagaggt tgccgacgaa cgggatgacc gcgaccgcgc agatgttgcc gaggatgggg
    15001 atccacaggt agacgctggc cgccatgccg acgccgtatt ccttgttcgt cgcataggtg
    15061 gcaccgaaga ccgaggtcgt cacggcgatg atgttgaaca actgcatcat gaacacccgc
    15121 aggatcgaca tgccgctgtc gcggaaggcc tcggcgatcg gggtttgggc cacgttgcct
    15181 tgggccttct cggactcgaa cgcgtgggtc tcctcgacgc ggcggcggat gatgtagccg
    15241 gcgaagacga cgaagacact gagcaggaac gggattcgcc aaccccacga cttgaacgac
    15301 tcgtcggaca tgaaggtggc gagcggcagg aagatcgcgg ccgcgaggat ctggccgaac
    15361 tgggtgccct gcaaggtgaa gctgccgaag tagccgcgtc gcccgaacgg agcatgctcg
    15421 aggatcatcg aactggcacc ggagatttca ccggccaccg cgaagccctg gaccaggcgc
    15481 agcacgagca gcagcgccgg ggcgaggaag ccgacctgct gataggtcgg caggatcgcg
    15541 acgaggaagg tcgaggcgcc catgaggagc atgcagagca cgaggacgtt cttgcgtccg
    15601 tgcttgtcac cccaggctcc gaggacgaag gcgccgatcg gacgcgcgac gtagccgacg
    15661 gcatacgtgc cgagggcgcc gatgatgccc atcttcgggt cgttcttgtc gaagaagatc
    15721 gacgggaaga ccaacgccgc ggcctgggcg tagatgaaga agtcgtagta ctccaaggcg
    15781 ctgccgatcc agccactggc cgtggccttc ttggtctgcc catggtcgtg cgtgacgcca
    15841 tgcgatgtgc tcattggtta cctccgtgag agtccgtcca ggcgcaatgc cgcctagtcc
    15901 gggtggggtc actgcggggt gcaacgtgcg gtgcagagtc gggtgcaagg gtctcgtgcg
    15961 cacagcttga gctgggttcg ggagcggtgg tgggccgggg ccgaccagga gggtcaggct
    16021 ccgccaacca tgtcgcgcag gtgccggtgc atacgttgag cgtcaggatg gacgcccgtg
    16081 aactggtaga acgcctcgac cgcctgttca gcggccattc ccccaccgtc gaccgtgcgg
    16141 cagcccttgg cgcgcgccac gcgcaacagt tcggtctcca gcgggaagta cacaatttcg
    16201 gcaacccagt gctcgggggt gatgagctcc tccggcacgg cgcacccagg gtgcgtcgcc
    16261 atcccggtgg gggtgcagtt gacgacgcct tcggcccggg ccatcgaggc cgccaggtcc
    16321 gtcccgacct cgacccggtc ggcgccgaag atggcagcca tccggtcagc caactcctgg
    16381 gcccgctcgg ggaagaagtc gtgcaggatg agcgggttgg ctccgtggtc cagcagggcg
    16441 taggcgactg cggctcccgc tcccccggac ccgacctgga cgacggcgcc gtggccgacg
    16501 tcgggcagtt gggcctggaa cggtcgttgg aaaccgcacc agtcggtgtt gtgaccgatg
    16561 gtccgcccgt cccggatcgc gacggtgttg acggcgccga gggccgtggc ccggtcggag
    16621 agctcatcca ggtaggggat gatcgcctgc ttgaacgggt gggtgacgtt gaacccacta
    16681 cagcccagcc gtttggccca ggtgagcacg tcgggcaggt cagcggcggt gagccccagt
    16741 tcgagagcgt cgacgatccg gtagctgacc ggcataccga gcgaagcccc ctcgcgctcg
    16801 tgcagcgcgg gggtccgtga cttcccgatc ccttccccga tgaggaacag gctgtgggtg
    16861 accagtggtt cggtgctcat gcaaggcctc ctgcgacagt gcagtgacgg gaaagggaaa
    16921 cagcgggtga agcggtttgt cctcgcggga ggcgaggcgg tcaagcagcg ggctgggcga
    16981 agtaggcctt ggcggtctcg aggcagcggc gggcatgccc ttcggcaccg cgttcgactt
    17041 cctcgcgttc gttgggcaac tcgatcgagt agatgagcgg gggcaggtgg gcgagggtgg
    17101 cggccgggtt gatcccgccc tccccgacgt aggaccgagc cgctcggagg atgtgcgtca
    17161 tcttctcgac gtccgcgggg atctcccccg gcgcgtcaca gaggtgacag aactcgaacc
    17221 actcgggcgg aaggccgtcg agatcctcga cgcggtcccg agaccggtgg aagtggtgca
    17281 ggtcgatcat caagcgggca ttgggccgct gcgtctcgcg gagcacgtcg accgcgccgg
    17341 cgaggttgcg cacggctgcg atcgggacga actcgaggtt gagcgtcatc ccatagctgg
    17401 cgaccaggtc ggccagccga ccgaaggcgt cgagatagaa cgggcgatct ggcacccaga
    17461 tgctcgtcag cactccagtg gcacccaact cggccccgac ggcgatctcg ggctcgtaga
    17521 cccgcgggtc caggccctcg acgatccggg ccaactcgat gtcgtggacc ggaatccccg
    17581 tgtccgccaa cgcggctcgg gtctgagcca acagcgtcgg gttggtggcc agggagtagt
    17641 catccccggg gagaccatga cagatcagcc gggggctgac gtagtcgtaa cccgttcggt
    17701 gagcgatccg gaccaactcg ggggtgggag tgtccaggat cgtcaggtgc gccaacgagt
    17761 agcgctgcgg ggccgcggtg cccatcgtct cgccttagtc ggtggaagcg tggtggatgg
    17821 acttgccggg gctgtagatg tccatgatgt tccagtgccc cgcggtgggt gtggggacgt
    17881 tgaccatgcg gacatcgatg acgaccggga cgttcattgc gacggcccgc tccagggcgg
    17941 ggcggaagtc ggcggcggtc tcgacccgga tcccggcgac tccgtatgcc gccgcgatcg
    18001 cggcgaagtc ggtctccacc ggtccgtcgg cgttctcgaa caccgtcccg taggtggtgt
    18061 cgtagtgggc cttctcgagc ccggcgatcg tgccgaacgc gttgttgttc atgacgaccc
    18121 agatcgccgc gatgcccttc tcgcgtgcgg tgtagagcag cgccgggttc tggccgaacc
    18181 cgccgtcacc gacgagcgac acgacgaccc ggtcgggaca ggcgatcttg gcaccgagcg
    18241 cggcaggggc accgaagccc atcgtggcga agccgccagg agtgaacacc gagccgggcg
    18301 tgaggatgtc catctgctgg gccaagccgt tcttgttcca gcccacgtcg gtcgtgatga
    18361 tggcgtcgcg cggcagcacc gcccggacgt cggcgaggat ccgctcgggc cgcatcggcc
    18421 acgcgtcact gctcgcgccc tcctgacagg tggccttgaa ggcagcccgg ttgcgctgga
    18481 tgtcggccag cacctcggtg cgctgcacgc cggccggagc cagcttcttg gccgctcgaa
    18541 cgagcgcggc cagcgccggc ttgaggtcag ccacggcacc gagcgccagc gggtagttgc
    18601 ggcccagctc cgccgggtcg atgtcgatgt gcatgaggcg ggtgggcggc atacggaagg
    18661 tgtaagcctc ctcccacgag ctggagtcgg cctcggagaa ccgggtgccc agggcgagga
    18721 tccagtcggc gttgcgggtc atgtcgttga tgaactcggt cccccagaaa ccggtcatcc
    18781 ccaacacgca cgggttgtcg tcggcgaccg cgcccttgcc catgagggtg tgggcgaccg
    18841 ggatctgcag gtggtcgacg agctcggcga gctcatcggt ggcgtcggcg aggacgaccc
    18901 cgcctccgac gtagagcagc gggcgctcgg cggcgaggag gttgccgacg atcgtctcag
    18961 cggtcgcctc gtcgagcgac gggcggacga gctcgtgggt gccggtcatc acgcggtcca
    19021 tgagggcgac gtcgatctgt gccgagaaga tgtccatcgg cacgtcgacg agcaccgggc
    19081 cggggcggcc gctctcggcg agggcgaacg ccttgtcgag gatctcgggg aacaggtgtg
    19141 ccgagtcgac ccgccaggcc cgcttgacga acggccggta gatctgccac tgatcggcgt
    19201 cggcgtggag gttgacctcc tggtgggggt gcttgccgta gtagtggctc ggcacgtcgc
    19261 ccgcgatgac gaccatgggg atcgagtcga gcgcggcatt ggccaccccg gtagcggcgt
    19321 tggtgaggcc ggggcccagg tgcgagagca cgaccgaggc cttcttggtc gcccgggcgt
    19381 agccgtcggc ggcgtgcgag gcgatctgtt cgtgccgggt gttgacgaac tcgatctggg
    19441 tgctgcgcga caacgccgcg aggacggcga tgttggtgtg gccgcatagt ccgaagacgt
    19501 gccgaacacc gcggtgctcg aggtagcgga ccagttggtg ggagacgaga tccgtggtca
    19561 tcgagactcc ttgtcggcgg tcggttcagt caggtcgagg tcgggaaccg cacgacgcgc
    19621 gggcccggtg tagcgatagg ggatggtctt gggcatcggg cccttgatcc gtccgccttg
    19681 gctgtgctgt tcccaggcat gggcgaggat ccccaccgag cgggagagga tgaagatccc
    19741 ccgcccgagc tcggtggcga accccaactc gctgtagatc acggccgtcg ccccgtcgat
    19801 gttcatcggg atccgggccg gtcggccggc gctgatggcg tcctccacgg cgcggccgat
    19861 ggcggcatac cggccgctga cgtcccctgc ggccacggcg tcatcgacca ggccgagcag
    19921 ccgcggagcc cgagggtcca acgggtgcca gcgatgtccg aagccgggga cgtagcgcac
    19981 cccctgagtg cgccactccc ccaggacctc ggccgttgct gtcgcgaggg ccgctccccc
    20041 atcgaccctc gcggcgatct cggagtagag ctccatgcat tgctgtccgg caccgccgtg
    20101 gacgtcgtcc aggacgttga tggccgaggc catcgcgccg ttgagcggaa ccccacacgt
    20161 catcgccatc cgcgagatgg cgatcgacgg ggcgtgcggg ccgtggtcga cagccgacac
    20221 gagggccgcc tccatgagcc gggcctgggg ccgggtcggc aactcgccgc gcaacatcag
    20281 ccagatcatc tcggggaagg tgacgtggcc gatgagctct tcgatgggat acccgcgcat
    20341 ggcgatgtag cccgggtgga tgtcgacgat gtccgtggac caccagtcct gggcggactc
    20401 gaccaccgct tcgagttgtt gtgcggtgcg ttcgctcatc cgactctcca acctctcgtt
    20461 taggggggtg acgtcgatca gggggtatgc cgtgccgtgc cgcgcgtggt gcgcagcggt
    20521 caggaggtga tgacttcgcc ctcaggctcg agcgtcgcgt cgtagaagtc gccgaagagc
    20581 tcctcgtcgc tgggcttgtc ctcggggatc ggcaccgaca cccacgtggt gttgaggtag
    20641 tgcttgaggt ggacgttctc ggaggtgatg ttgccgcccc atgtgccaca gcccatcgag
    20701 gaggtcatcg gcatcccgtt gttgaaggcg ccggcgttgg ccttggactg gggctgacgg
    20761 accatgatcc ggctcaccgg cgcagccagc ccgaggcggt ggatgtggtc ggcgtcatgg
    20821 gagtagatgc cgcacgagtg ccccttgccg cccacctcgt agatggcccg catggcgtcc
    20881 agcgcgttct cgaactcgcc ctcgtagcgg tggacggcga gcagagtggt gagcttctcc
    20941 atcgagaaga cgtgctcacg gctgatcccg tcacccttga cgaagatgaa ggtccggtcg
    21001 gtggggatct cgaagccggc cagtcgggcg agcttctgcg gggcgaccgc caccgagtcg
    21061 ggcagccggt gcttctcggc atcccacatg acggcggcca gcgcgtcacg ctcggagccc
    21121 tcggcgaggt agccgccctc ggcctgcagg gccgtgatca tctcgtccca gatcgactcg
    21181 tggatgatga ggttgccgtc ggcagagcag cccgagccga agtccgaggt cttggagatc
    21241 cgggagtttc gcgcggcgat cgccacgtct gcggtctcgt cgatgatcat cgtcgagttg
    21301 ccggcgccga cgccgtaggc gggtgtcccc gagctgtagg ccgctcggac catcggctgt
    21361 ccgccggtcg cgatgaccag gtcggagcgg gccatgaggg cctgcgacac ggggatgctc
    21421 ggcagggtga tgcactgcag gatgtcggcc ggagcgccct cgcgctcgag cgcctcacgc
    21481 atgagccgga ccgtctcgaa ggtggtcttc ttcgaccgcg ggtgcggcga gaagatgacg
    21541 acgtcgcgcg ccttgatggc atagatcgcg ttgcccgccg gggtgaggtc ggggttggtc
    21601 gtcgggacga tgcagccgat gatgccggcc ggcttgccgt acttcaccag gcccttctcg
    21661 gggatctcct cgatgatgcc gacgctcttc tggcgcaggg catcacgcag cacgccccgg
    21721 atcttcatcc gcttgcccat ccggctcacc gggtcgccga ggccggactc ctcgatgcct
    21781 tcggccacca accgggtgaa ggtcaccttg ttggacacgg cccaggcgac cgcctggcag
    21841 agccggtcga cgcgcgcctg gtcgtaggtc tcgatgatgg cgagcgcggc gcgggcgcgg
    21901 gcgaagatct cgtccagttc ggcctgctga tcggcggtga gttccttctt gtcagccatg
    21961 gtgatgcctc cggcgcgggg tgttcgggga aacggacggc tacgggatgt cccggacgag
    22021 ctatggggta tgccgtccgg ctgggtccct gggcgtccct gcgttggagc ggctcccctt
    22081 cagtgtggag gactcagccg acttatgcac tatctagtta gttaccaata atgtgatctg
    22141 cgtcacatac ctcgtccggt tagggtcggg cccatgtcac accagtcgtc cgctgccctg
    22201 ctggcgctgc atgccgtccg ggtgaccggc atggcatcca ccgagcgggc cgccgcgcgc
    22261 tacggactcc cccgggccga gacgcacgag ttgcttgagg acgaccgggc ccgcggttat
    22321 gtccgccggg tcgagttcgt ggatctggcg ggatggaccc tcaccgagtc cgggcgcacc
    22381 gaggacgagc gccgcctggc cgccgagctc gcgctgactg gggcccgccc ggtggtgcag
    22441 gccggcgccg acgacttcgt cggtctcaac gcgcgactgc tcacggcgat caccgactgg
    22501 cagttgcggc cccaaccgtg ggaccggctg gccgcgaatg accacaccga ccatcgctgg
    22561 gatgaccggg tgctgcgtga gctcacccgg atcggcgagc tcctcgcccc ggtctgcgac
    22621 ccgctggcag cggccctgag ccgattcgag ggttacccac agcggtatgc cgccgcgatg
    22681 gcccgagtgg ccgacggcga gcgctcctgg gtggatcggg tcggaccgga ctcggcccat
    22741 ggggtctggc tggaactgca cgaggacctg cgtgccaccc tcggggccgg gccgtgcgtc
    22801 acgccgtctt gagctcctgc agggcgaagg tcatcgccag gttgtaaccg agcgtgccga
    22861 agccggccat caccccgacg gcgatgtccg agaggtagga gtggtggcgg aacggctccc
    22921 gcttgtggac gttcgagatg tgcacctcgg cgaacggcac ctcggcggcg gcgaacgcgt
    22981 cgcggatcgc cacgctcgtg tgggtgaaag cgcccgggtt gatgatgatg aacgccgtcc
    23041 cgtcggtgcg ggcggcgtgc agccgctcga cgatggcgcc ctcgtagttg ctctggaagc
    23101 actcgacgct caccccggcg gcctcacctt gagcccgcac cgcggcctcg acgtcggcca
    23161 gcgtcgtgct gccgtagatc tcgggctggc gagtcccgag catgttgagg ttcgggccgt
    23221 tgatgagcag gaccgacatg gcgttctcct cggtgtcgtg cgttggcggg gtctacagat
    23281 cgggtcgggt gcggggccgc cttcaggagg cgagctcggc gaaggcggct tccagcagtg
    23341 cttcgtcggg cccttcgagg cgcccaggcg tggccaggtc atcgagcacg acgaaccgca
    23401 gcagcgctcc ccggctcttc ttatcgcggc gcatcgccgt gagcaaccgc ggccaggcct
    23461 gcccgcctgc gctcgggtag gtcaccggca gcccgagcga ggtgaggatc tcggtatgcc
    23521 gtgccgccac gtcagcgggc gtccgcccgg cgaggcgacc cagcgccgcg gcatacacca
    23581 gcccgaccga caccgcgttg ccgtgccgcc acgagtagcc ctcgacctgc tcgaccgcgt
    23641 gcgcgaaggt gtgcccgtag ttgaggatct cgcgcagcga cgactcgcgc aggtcggcgg
    23701 cgacgacggc ggccttgacc cggatcgagc gctccaccaa ctcggccagg acgtcgccgg
    23761 cgggatccag ggcggccgcg gggtccgcct cgatgagctc gagcgtgcgg gggtcggcga
    23821 tgaagccgtg cttgacgacc tcgcccattc ccgcgacgag gtcggcgcgc ggcagggtgc
    23881 tcaaggtggc gatgtcgcag accaccccgg ccggggggtg gaaggagccg acgaggttct
    23941 tgccctcggc ggtgttgatg ccggtcttgc cgcccaccgc ggcatcgacc attccgacga
    24001 gcgaggtcgg cacctggacg accgcgaccc cccgcagcca ggtggccgcg atgaagcccg
    24061 ccacgtcggt cacggctccc ccgccgaccc cgatgaccgc atcggagcgg gtgaacccgg
    24121 cctgaccgag caccccccat gcctgcgcgg cgacgatggg cgtcttggcc gcctccgcgt
    24181 cggggacctc gtgcaggtat gccgtccgcc caccggcctc gatccgcgcg gcgagcagag
    24241 cggccaggtc gcgcccagtc ggggcgtgga cgacaagcac cttcgcgacc cagtcggcca
    24301 gcaggtcgac ggcgaaggtg gcgatccccc ggccgacgag aacgtcatag tcgtcgccga
    24361 caccgacgcg gacgagatca gccatgctcg gacgccccga cggccagatc gtccagcgcg
    24421 ccggcgagtt gctcgacgtc cggtgacacc ccggcttggg cccggccaag ggcgtccagc
    24481 aggacgaacc gccaaccctt gcggtatttc ttgtcnnnnn nnnnnnnnnn nnnnnnnnnn
    24541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24601 nnnnnnnnnn nnnnncgttc gtcggccgtg accacggccg ccttgatctc gacactgcgc
    24661 cgcacgatct cgacgagggt ctgaccgtcg cgagcgacga tcgcgtcccg gcgctcgcgg
    24721 agcgtgtcga gcagggcggg atcttccagg agggcgtgct tgacgatctc ggccagcccg
    24781 gcgcgatagt cggcatcgcc gtaggtcgcc gacaggaccg ccaactcgat gtcgcacacg
    24841 acgaggacgg gctggtggat cgtgcccagg aggttggcgc cgtggggcag gtcgatcgag
    24901 cacttgccgc cgatcgccga gtcggcctgt gcctcaaggg tggtcggaac cagggcgtgt
    24961 gccatccccc ggttgtaggt cgccgcgagg aagccggtga cgtcgcaggt cgcctccccg
    25021 ccgacgccga cgagcaggtc tccccggtgc accgacaact ccgcgagcgc ggaggcggcg
    25081 aggccgaccg catcgagggt cttggccgtg ctccccgacg ggagggtcac ccgacgcacg
    25141 gggatcgcca gcgtgcgcag ttgcgcgacg atctcggcag ccgcctcacc ctcgtcggcc
    25201 gttgccagga tgacgatctg ctcggcatcg ggcatcggcg gcagcagcgc ggcgatctgc
    25261 gaacggatgc cgcgaccgac gtggacccga taggacccgc caccgggggc cacgaggacc
    25321 cctgccgtcc cgtcgagggc agccgtgccg gtgatcgcgt cgatgatctc gagcccgatg
    25381 tcctcgaccc gccggccggt cgtgcgcgag gtgatggtgg ccgtcgccgc gtagatgtcc
    25441 aggcgggcgg catacagggc gggcaggccg gcgtcgtgca ggagcgggcg gtattcgtcc
    25501 cgcccgaccc gagccagggc ctcgtccaac tcgacgtgca gatagatgac cgtgtgacca
    25561 cgcagccgct cccgagtctc ggctcgccca caggcgccgc cgccgaggga caacacgcag
    25621 gcaggtccgt cgagcaactc ggcgatggtg gccgcttcga cgtcgcggaa gcccgactcc
    25681 ccctccgcgg cgaagatgtc cttgatcgag cgccgctgct ggtgctcgat gaccaggtca
    25741 ctgtcgacga acggcagccc gaggcgttca gcgatgaggc ggccgaccgt ggtcttgccc
    25801 gctcccatga atccgacgac gacgatcacc gcacgagcct cctcaccagg tccgcatccg
    25861 ctcggggacg gccgcgaggt atgccgcgtg gttgcgtgcc gtctcggcga cagagtcacc
    25921 gccgaacttc tccacgaccg cttcggcgag cacgagggcg accatcgcct cggcgacgac
    25981 tcccgccgct gggaccgcgc agacatcgga tcgttggtgg atggccttgg ccggctcgtc
    26041 ggaggccacg tcgatggtgt caagcgagcg cgggacagtc gagatcggct tcatcgcggc
    26101 gcggacccgc aggatctggc cggtcgacat gccgccctcg gtgccccctg cccgaccggt
    26161 gcgccgctcg atgacaccgt cgtcgcggcg ggccatctcg tcgtgggcgg ccgagccgcg
    26221 tcgtttggcc gtctcgaagc cgtctccgac ctcgacaccc ttgatcgcct ggatgcccat
    26281 gagggccccc gccagccgcg catcgaggcg ccggtcccag tggacgtggg agcccaggcc
    26341 cggcggcacg ccgtaggcga gtacctcgac gaccccaccg agggtgtcac cgtcgcgccg
    26401 ggccgcctcg acctcggcga ccatggccgc cgaaccttcg gcgtcgaggg tgcggaccgg
    26461 gtcggcgtcg aggcggtcga cgtcgtcggg atggggcaac gcggccgtgt ctgcgaccgc
    26521 ggccgtgccg atggcgacgg tgtgcgacac caaccggatc ccgaccgtct gctcaaggaa
    26581 cctcgcggcg acctcgccca acgcgacccg tgccgcggtc tccctggccg aggcgcgttc
    26641 gaggatcgga cgggcatcgt cgaagccgta cttctgcatg ccgacgagat cggcatggcc
    26701 ggggcgcggc cgagtgagcg gccgattgcg agcgatctcc ttctcggcgt tgacgtcgtt
    26761 cgcggcggca tacgcgtcgg gatcgaccgg ctcggggctc atcaccgtct gccatttggg
    26821 ccactcggag ttgccgaccc ggacggcgat cggcgagccg agggtgaacc cgtgccggac
    26881 gccaccgaga aactccacgt cgtcctcctc gaaggccatc cgggcccctc gcccgaaccc
    26941 cagacggcgc cgggccagag cccggcccag gtcggcacgg gacaccgaca cccccgcggg
    27001 caggccttcg aggatggcga tgagggcggg cccgtgggac tccccggcgg tgagccaacg
    27061 cagcatgctg gcatcctccc acgcagcctg ggccaccggc ccgacgaccc ggatggcaag
    27121 acgtcacccg agcaggacag ccaccagcgc cccgacgagc agatacggcc cgaaggccag
    27181 gtccgtgcgc atggtgagcc gccgggtcgc caaccccacc accgagacca ggccgctgat
    27241 gacgaacgcg agcatcaacc cccaccacgg caggcgtccg tcgacgctgc ccagggctat
    27301 cccgaggatg cccccgaccg tcacgtcacc gaggccgaac tggccccgcg agatgagggc
    27361 gaggacgagc aggacgaggt atgccgccac gccgctgacg agcgcgcgga cggggagcgt
    27421 ccaactcccg gacacggcgc cggcgaccag gacgatcccc agcacgcccg cacccagcgg
    27481 ataggtcagc ccgtgcggga gccggtggac atcgatatcg atccacgaca acgccacccc
    27541 ggcgaacccg acaagcaaca aggccggcag caaggtcagg gtgcctggcc ctccgacccg
    27601 ccacgcgagc aggccccaga cgaggggcac gagggcgacg agcagccacg gtcggcgcag
    27661 caccggccgg tcccattcgt cgtccaggcg atagccgccg gtaaccaact cccgacgcag
    27721 ccagccgccg agggcagacc cgaccaccac ggccgcgagg acgagccacc aggggccggt
    27781 ctgggtgaag acggtcattg gaggtcccct ggctgcggtg ccatcacacg tcggcggcgg
    27841 ccagcagggc cgcccgcatc gcctcggtcg gagcatcgcg ggaggtcatc agtcgcacct
    27901 gctctcgcgc ctggtgcagc aacatgtcgg tcccgccgac gacggcatac ccgatctcct
    27961 gggcgacccc gagcagcggg ctcgggccgt cgccgtagac gcagtccagg accgccgacc
    28021 cggggtgggc ctccggcagg gcaccgagca gaccggcata cgccgtcgcg gggatggtgc
    28081 tgacgatgac gtccgtgccg tagggccaat gccccatcgg cacggtcacg gcatcgagac
    28141 ccagatcgcg agcgaaggcg agggtgccgg ggcgcacggc gtcccggacc atgaagctca
    28201 cctgcgccgc accgaggtcg gcaagcgcgg ccagggccga gcgagcggtg gcgcccgctc
    28261 ccacgaggac ggccgagcgg acgtgcccca cgccggcttc ccgcagcgcc tcgacgatgc
    28321 cgtagacgtc ggtgttgtgc cccgaccagc ccgcctcgtc acgcaccaac gtgttgatgg
    28381 cacccacctg gacggccagg tccgacgccg tatgcacgac gtcgaaggcg gcctccttga
    28441 gcggcatggt cagcgacagc ccgcgccagg tctcgtcgag ggcggccacg tgggtcgcca
    28501 actcgtcggc ctcgacccgg atccggtcgt agctccaatc gccgagcccc aacgctcggt
    28561 atgccgcgcg gtgcagcacc ggtgacaggg agtgttcgat gggcgaaccc cgaaccccgc
    28621 atcggttgtg caccacagac ctcatcgaga gcatttcccc tggttctccg ccgcgttgca
    28681 ccaggcttgg aactgcgcaa cgtaggtgtc atgctcggcc tgggtggcgg cgaacttggt
    28741 ctcgcccgtc tcggggttga ccgcgacgaa gaagagccag ggtccctcgg ccggcgccac
    28801 tgcagcctgg atcgaggcga gcccggggtt ggcgatcggg ctgtccggca ggccgtcctt
    28861 ggtgtaggtg ttccacccgt tgacgctggc gcgttcttcg tcggtggtcg tgatggcccg
    28921 cttcttgacg ccgtagctca ccgtcgagtc catctgcagg cgcatcggct tggccagccg
    28981 gttgagcacg acccgggcga ccttggcccg gtcctcggac cgcttggcct cagcctcgac
    29041 gatgctggcg atgatcatga tccgctcggc ctgatcgggt tggatctgca ggtcggtaag
    29101 gaccttgacg gtcttgccga ccatggcggt gagttgggtg acggcggagt ccttgtcgga
    29161 gaactcgtag gtcgcgggga acaggtagcc ctcgacgttg ccgccggccg acgggggcag
    29221 cagcgccaac acgtcggggc tcttggccgc agcctcgtag tcggccaccg aatgcttcgt
    29281 ggccttgccc aacaaggcat agatctcgct cgcccagagc ccctcacgca cggtgacctt
    29341 ggggttctgt cggttcttgg ggtcgacgag gatcgccaac gcgtcggcgg ccttcatctc
    29401 cttcttcagg gcgtagtcgc ccggttggat cccggcgcac cgcgagtcgg cgctgcacgc
    29461 gtcgacgaac gccttggcgg taaggacgat gccctgggtc tgcaggttgg atccgatcgc
    29521 gcggcccgta tcgccgtcgt tcaccgtaaa ccgggccgcc gcaccccccg gaccggcaaa
    29581 gtctgacgcg tccgcggagc ggtcgaacaa cgggcgcagc gcggtgaaac cgaggaatcc
    29641 ggcaccggcg atgagcccca gcgccacgaa gaggatgagg aaccggcgca ccccacgacc
    29701 ggtgttgcgc cgctcccgcc tggcccggcg tgagtcttcg gcgaacccga aggccgctgc
    29761 atcgggatcg tggtagccgc cgtgcccctc ctccacaccg tcgtgctcgt actcgtgctc
    29821 gtacgcgtac tccccctcgt gcgcgtcgtt ccccgcgtgg gcgtactcgc cttcgtggac
    29881 atcgggccct tcgtgggcgt acccaccggc gccggaatag tcgacctcgt gagcgtcata
    29941 gccctcatgt ccggcgtgca cctcgtgctc gccggcgacc tcccccgcgg cctggcccca
    30001 gacgtcgtcg ccgcggggat cccgcagcgg gcgctcggtc atgctcgtcc cttcgtccgg
    30061 ggcttacgcc cgcctgtgct gaccagctcc cccggttgag acccggtagc ccgctctgcc
    30121 tcgagtgctg actcgaggat gagcactgcg gccacctggt cgacgacggc tcggtgcgtg
    30181 cgttccgcgc ggccactgtc gtgcaacagc cggtgggacg cgacgctggt gagtcgctcg
    30241 tcgaccaacc gtacgcggac aggggccacg agcgcagcaa gctccgcggc atacgccctg
    30301 gcttgtcgcg ccgattcccc ggaaaccccg gagagttgca gcggcaaacc gacgtagacg
    30361 cacgtcgcgt cgcggtcgcg gaccaacgcc gcaagcgcgg ccagatcggc gtgttcgggg
    30421 ctctcgtcgc gggccagcgt cgtcaccgga gtggcgagta tgccgtcggg gtcgctctcg
    30481 gctaccccca cccggacgga cccgacgtcg acgccgaccc gtcggccctg cgacggcata
    30541 ccccgttaac cctgggcccg aagcgagtcc tcgacggcgc cgagcgaggc acccatggag
    30601 gcggcgtcga cgccgccgcc ctgggcgagg tcgtccttgc cgccaccgcc accgccgagc
    30661 acctgggcgg ccaggcggac cagcgccccg gccttgactc cgccctggcg agccggtgcg
    30721 ttggtcgcga cgatgacgac gggcttgccg cccatggacc cggtcatggc cacgacggtc
    30781 gcgtgggcat cgccgagccg gctgcgcacg tcgagcacca gcgagcgcag atcgtcggga
    30841 gcgatgcctt cacccgcgtc atgggtcacc agtcggaccg acccgatctc ccgtgccgac
    30901 gagacgagcc ccggagcgac cgcgagagcc tgctgctgac gcgcggcggc gatctcgcgt
    30961 tcggcgtcgc gcagcttggc aaggatgccg ctgatccgct cgggcaactc ctccggccgg
    31021 gccttgagcg cctcggtgag ttggccgacg atggcgtgct cacgggcgag gaaggagtag
    31081 gcgtcggcgc cgacgagcgc ctcgactcgc cgcacccctg agccgatcga cgcctcgccg
    31141 agcagcttga cgacgcccaa ctggctggtt cgaggggtgt gcgtgccacc gcacagctcg
    31201 cgggcccagt cgccgaccga cacgacgcgg acggcgttgc cgtacttctc gccgaacagg
    31261 gccatcgcgc cgatctcgcg cgcctgatcc tgggtcatca cctcggccga gaccgggagg
    31321 tcatcgagca ggacggcgtt gacccgggcc tcgatctcct gcaactgccc caaggggatg
    31381 gcgtgttgag cattgaagtc gaagcggaac cgcccgggtc cgttctccga cccagcctgc
    31441 gtggcggtgt cgcccagggc ctcacggatc gctttgtgaa ccatgtgggt cgccgtgtgg
    31501 gcacgcgaga tcgagcggcg ccgctcgacg tcgacctccg cctgcgcctg ctcaccgacg
    31561 atgacctcgc ccgcgatgat cgtcgcgcgg tggaccgaca gcccggtgat cgggctctgc
    31621 acgtcgcgga cctgcacgat cgcgccgttg gccaactcga tccggccacc gtcggccaac
    31681 tgcccgccac cctcggcata gaacggggtg cggtccagga ccagctcgat gtcctccccg
    31741 gcgatggcct ggccgacgct gcgcccgtcg cggacgatcc cggcgacgcg ggcctccgac
    31801 acgacctcgt cgtagccggt gaactccaca ccccgcccga gccggtcggc gacctcgcgg
    31861 tagaccccgg tgtcgccgtg ggcgctcttc ttggccttgg cgtcggcctt ggcccggtcg
    31921 cgctgctcct tcatcagccg gcggaagccg gcctcgtcga cctgcacgcc ttgctcggcc
    31981 gccatctcca gggtgaggtc gatggggaag ccataggtgt cgtgcagcgc gaaggcctcg
    32041 tcgcccggca gggtcaggac ctcgcccccg gtgcccttga gccgggcgac cgcgccgtcg
    32101 agcagcgtgg tgcccgaggc gagggtgcga cggaaggctt cttcctcggc atacgcgatc
    32161 gtgctgatcc gaccgaagtc ggtgcgtagc tcggggtagg acttgctcat ccgctccatg
    32221 ctcaccggaa gcaggtgcgg cagcgccggg tcgtcgtagc caagcagccg catcgagcgg
    32281 acggctcggc gcagcatccg gcgcaggacg tagccgcggc cctcgttgcc gggggtgacg
    32341 ccgtcaccga tgagcatgag gctggagcgg acgtggtcgg cgacgacccg caaccgcacg
    32401 tcgtcggggt ggctgtgccc ggcgtcgcgg ccagagttgg cgccgtagat cttgccggtg
    32461 agctcggccg ccttgtcgag gaccgggtag acctcgtcga tctcgtagag gttgtccacg
    32521 ccctgcagga tcgcggccat ccgctccagg cccatgccgg tgtcgacgtt cttggccggc
    32581 aggtcaccga ggatctcgta accctccttg gccccgccgg tgccccggat gttctgcatg
    32641 aacacgaggt tccagacctc gaggtagcgg tcctcgtcga cggccgggcc gccctcgcgg
    32701 ccatagtcgg gcccgcggtc gtagtagatc tcactgcagg ggccacacgg cccgggcaca
    32761 cccatggacc agaagttgtc ggccatgccg cgcttgacga tccgctcgcg cgggatcccc
    32821 gtgtgcttga cccacaggtc ggcggcctcg tcgtcgtcca ggtagaccgt cgcccagagc
    32881 cggtcgccgt cgaggccgta gcccccctgc tgctgcgggg tggtgagcaa ctcccaggcg
    32941 gccttgatcg cgcccttctt gaagtagtcg ccgaaggaga agttgccgtt catctggaag
    33001 aacgtgccgt gccgagaggt cttgccgacc tcctcgatgt caccggtgcg cacacacttc
    33061 tggacgctcg tggcgcggtc gaacggcggg gtctcctggc ccaggaagta gggcttgaac
    33121 ggcaccatcc cggcgttgac gaacagcaag ttcgggtcgt cgtagagcaa cggggcggac
    33181 gggacgaccg tgtgcccccg cccttcgaag aaggcgagga atcggcgcct gatctcggcg
    33241 gtctccatgg tgggtccttg cgttcgggtc accgtatgcc gggtgctcac cggcgcggtg
    33301 aaggtgtggt tcgggggcgg gcagcgtcgg ggtggccacg cggtcaggct gggggcgggc
    33361 tacccctgac gagctcgcca gccggccggg tcgtcgagca ggtcgcgggt ggcctgcggg
    33421 tccaggcggg tccccgacgg cggggaatcc agccccaggg ccaggcgcag ttgttcttca
    33481 cgctcggtca tgccggcccg gatgtcagcc accacggctc cggcctcggc gagggccgca
    33541 gcgaggacgt ccggtggccc gccctccgtg gaataccgat cggccaggag ccgataggcg
    33601 tagacggcac cgaccccgac aagggcgccg gtggccagga ccagcagttt gcggctcatt
    33661 gctcgtgcct gcctttgcgg gtcgagccgc cggaggctgc ggtggtcgcg cggcgttggc
    33721 cgacggccga gcggacgccg tagctgaagg cagcaacctt caccagcggg ctcccgagcg
    33781 tggcggcgaa cagcgaggtg agggcgttga cgttgctgga catgctcgcg atgttggtgg
    33841 tcacgacgtc gatccgggcc aactgttcgt tggtggtcga cacggtcgtc gtcacctcgc
    33901 cgagcagcgg aacactgccg tcggccaacc ctttgacccc gatgcgcgtc tcgtcgagga
    33961 tctcgcccgc cttgccgacg accgatccca accgccacac gaggtaggcg aaggcgagcg
    34021 cggcaatgac accggcgatg tcgccgatgg tcacgggcgc gatactcacc ggttcaccct
    34081 agccgaacgg cataccgggc tgcgcgcggt tgacgcgcgg ccggcgggcg gctcagcgcg
    34141 tgacctcggc gaccagggtg ctcttgatct ccgggaagtg acaggcgatg acgtgctcgt
    34201 cgcctgtgcc gggcacgtcg tcgtcgcccc cgccgggctt gtcctccaac ggcggctcct
    34261 gggtggcaca gatctcctgc gccttccagc agcgcgtgcg gaaccggcaa ccactgggcg
    34321 gactgatcgg gctcggcggg tcaccgacca gccggatccg ctccttgggg ggtatgccgc
    34381 gcacgacgtt gatgtcgggg gcggcagaga gcagcgcctg cgtgtagggg tgctgcgggg
    34441 tgccgtagat gtgttgcttg ttgcccaact cgacgatctt gccgaggtac atcaccgcga
    34501 cccggtcaca gaagtgccgg atgaccccga ggtcgtgggc gatgaagatg aacgacaacc
    34561 cgagctcgga gcgcagccgg gacaacaggt tcatcacctg ggcctgcacc gacacgtcca
    34621 gagccgagac gggctcgtcg gcgatgatga gcttgggctc gaccgccagc gcgcgggcga
    34681 tgccaatccg ctggcgctgg ccaccggaga actccgacgg gtagcggttg atgtgctcgg
    34741 ggttgaggcc gaccaactcg aggagttcct gcgagcgagc cttctccttc cccttggcca
    34801 ggccgtggac ccgcagcggc gtgctgagga tcgcgccgac cgtctggcgg gggttgagcg
    34861 agctgtaggg gtcctggaag atcatctgga tctcgcggcg aagcggacgc agctgcttct
    34921 cggggagcgt ggcgatgtct cggcccttgt aacggatcgc gccggaggtc ggcgtctcca
    34981 gccgggtgag gcagcgggag acggtggact tcccgcagcc cgactccccg accagcccga
    35041 gagtctcgcc ctgagcgagg gtgaaggtga cgtcgtcgac ggccttgacg gtgttacgga
    35101 ccatcgtcag gccttgcacc tcacggactg cgaagtactt gacgaggtgt tcgacctcga
    35161 ggagcggcgc cgacgcggag cccgggttgg cggtcatgac accacttcct ggtcgagcac
    35221 ggactccgga ttggtcaggt ggcagcggat ctggcgaccc gggctgctga cccgcagctc
    35281 cggcaggagc gtcgcgcagg ggtcgccggg cactcggtct ttcaccatgc accgcgggtg
    35341 gaacgaacac ccggacggaa ggtgcaccag gctgggcggg ctgcccttga tggtctgcag
    35401 ttggtcgctg tcctcggagg ccgacggcag gctctgcagg agcgcgtggg tgtaggggtg
    35461 ctgggggcgg gccagcacct ccttggccag gccacgctcg acggcgcgac cggcatacat
    35521 gacgaggatg tcatcggaca cctcggccac gacgccgagg tcgtgggtga tgaggatgac
    35581 cgccgacccg aactcgcgct ggaggtcctt gaccaggtcg aggatctggg cctgaacggt
    35641 gacgtcgagg gcagtcgtgg gctcgtcagc gatgagcaac ttggggttgt tgaccaaccc
    35701 gagggcgatc atcgcccgct ggcgcatccc gccggagaac tcgtgcggat actgcttggc
    35761 ccgccgggcc gggttgggga tgcccaccaa ctcgagcatc tcgacggcgc gcttggcggc
    35821 gatgtccttg gagacgctgt tgtgcgcgag gtaggcctcg gcgatctggc tgccgacctt
    35881 gtagaacggg tgcagagccg agagcgggtc ctggaagatc atggccgcgg tgttgctgcg
    35941 cagccgccgc agcaacgact cgtcggcacc gacgatctcg ttgccgtcga gcaggatgct
    36001 gccggtgatc gtgctgcgct tggggttgtg caggccaagg accgccatcg acgagaccga
    36061 cttgccggag ccggactcac cgacgattgc cagggtgcgc cccagccgag cctcgtaggt
    36121 gaggtcctgg acggcgttga ccgggccttc gtcggagggg aagcgcacgc agaggtcggt
    36181 gatcttcagg accacctcac cggcgggtgc ctcggtcacg acgttgcgct cgggggcgga
    36241 cgcggcggcg acgtcgtgtg ccgctgaatc ctgcgggggc acgggctgag cgctcacgaa
    36301 agcctcacac gcgggtcgag gaaggagtag gcgatgtcga cgatgaggtt catgacgacg
    36361 acgagcacgc agccgatgag gacggtgccc atgatgatgg gcaggtcgtc attgcgcagc
    36421 gactcgatag cgaacttgcc gatgccatcc acgccgaaga tgtgctcggt gacgagcgtc
    36481 ccggacagca ggccggccat gtccaggccg aggatcgtca cgacggggct gagcgcagca
    36541 cgcaaggcgt gtcgatagac gaccttctgc tcgctgatgc ccttcgaccg ggccgtgcgg
    36601 acgtagtcct ggttgagggt gtccaccatc gaggccctgg cgtagcggac gtagccggtg
    36661 gcatagatga ggccgaggat gatcgccggg gcgatcaggc cgacgaacca cgggcccagg
    36721 ccgtcggacc ggttggcgta gcgcggcagg atcgggtaga gcacggtggt gtagagggcg
    36781 aacaacagac cgaggatgta ggaggggatc gagccgaaga cctggctgaa cccgaccacc
    36841 ccgcggtcta acgcggtgcc tcgtcgtcga gcggcgagga cgccggtgct gatcccgacg
    36901 gtgaggaaga tggccatggt catcaacgcg agcattgccg tgttggggaa cctgctggcg
    36961 agggcctctt tgacttcgat gccggtgcgg aacgagaagc ccaagcacgg cgccgaacac
    37021 gtcctcggtg ccccggacac atagatctcg cggccgacag ccaggccctt gaagtactcg
    37081 gtgaactgct cggcgaccgg gcggtcggtt cccaggcttc gtcggatgtc ctccagccgc
    37141 tggggggtgc aacgcaactc gccacacatc gcctgagccg ggtccgaggg accggcgaag
    37201 aacagcagga acgtcgcgac gagtacgacg aggaggacgc tcacggccat cagggcacgc
    37261 cgagcgatgt agatcagcac gtggacctcg cacgagtgga tgggcggtgt gcggggcgtg
    37321 gggtggtccc cacgccccgc acacacgggt tgctatcagt cactgagcat tgcgctcagc
    37381 tcactgctcc acccaaatcg catccaggac aggcatgccg tggttggggt cgttgatgac
    37441 gttcttgacc ttggtgccga acaggaggtt cgagcggtcg tagtagtacg ggatcgccgg
    37501 caggtaggtc tccatgagcc acttgtcgaa cttgccccac tccgggccct ggtcggcgat
    37561 cgacagcttg agaatgcgct ccatctcggc gtcgatcttc gcgtcggaca ggttgcccca
    37621 gttagtgccg ccacccttga tctgcgtgga ggacaccatg ggcgggaaga tcgagtcagc
    37681 cgacggccag tcgaagcacc atccaccagg ggactggagc atgttgtgcg ggccatcgag
    37741 cttggagatg agggtacgac gctccttgtt cggaacaccg aggtccttca ccgtgaagcc
    37801 ggcttcctgc agcttggtct tgcggacctg gttgacctgc tgggcaacgt tgctcgggtc
    37861 gtcgttggtg tagtagtaca ccagctcgaa gccctccttg ccggcagcct tgagcatctc
    37921 cttggccttc accgggtcac cattgcccgt gccggtcaga ccggggagct tgtagtccaa
    37981 ccagcccggg atctgcggcg ggatgagcgt gctggcgggc gtctgcgaca gcggtgtggc
    38041 gccggcagcc ttgttgatgt cgtcgaaggg gtaggccaca gcgatggcct tgcgaacctc
    38101 gagcgggatc ttgcggctgt ccatgttgac cgcgacgaca cacgaggact ggccgttgat
    38161 gaactggctg gacttggcgc cggtgacctg gtcggccagc gacgcgtcga tgccgtccca
    38221 gttgagggtc gtcgcgtccg ggccattgct ggcgaggatg ccctgctgca ccttgatgga
    38281 gtcctggccg aacttgaacg cgtagctgtc gacgtaggcc gtacgcgccg ggtcggtctt
    38341 cgcgtcccag ttggggttgc gcacgagggt gagctcggtg ccctgggtga acgacttgat
    38401 catgtaggga ccggtcgaca gggcggtgtt gccgtagtcg cggttcttgg tctccttggc
    38461 cttggggatc ggcgagacct gggtgaaggt ggcgaagtag ggcagggtct cccagcgctt
    38521 ctcgaggtgg aagatgacct tgttgccctg ggcctcgacg ccggcgaacg tgtcaccgaa
    38581 ggggttctca gtctccgggt tgtcccaggg accttcgtac ttggcaccgt ccttgaggaa
    38641 gtcgacctgg taggtcgggc ccgtgtcggt gaacgcgaag gaccgcttca cggcatacgc
    38701 gatgtcgtcg gcgacgacag cggagccgtc ggagtacttg atgccgtcct tgagggtgaa
    38761 ggtccaggtg aggccgtcag cggacacctg gccgaggtcg gtggcgaggt ccggaacgag
    38821 gacctgcttg ccgttgcggt tgacgaagga cgtcagcgag cgctgggtca gccgggtcat
    38881 gatcgccccg gagtcctggt agaactgggc gctcgggtcc atgtccgccg gagcggtgga
    38941 gtacgaaacg gtgagcgtgc cacccttctt ggctccggca acctccgggg cgggcccctt
    39001 ggccgtcggg tcgatcgcgg cggcggcctt ggattcggcc gtctgcgacg cggtcgcacc
    39061 cgtcgtcgcc ccaggggtcg acgcgggggc ggagcaggcc gccgtcaggc ccaaggacgc
    39121 gaccgccccc acggccagga cgtgagtcca gcgcatgtgt tttctccttt gatgggatgc
    39181 ctcgagaggt tcctcaaggc tggttcgaca gcagcgtggt tgacgctacc gacgggtgtt
    39241 ggggtcgaac gcatcgcgga tcgcttcgcc gagcagggac agggcgagga cgagcagggc
    39301 gatggacagg accggtggcc acaggaacaa ggggtagcgc gcataggcgt tctgcgcgtt
    39361 ggagaccgtg cggccccacg acggtgtcgg ctcgacgagc ccggcaccga gataggacaa
    39421 ggtggcctcg gccgtgatgt aggccggcac ggcgatggac gccgaaacga tgatcgggcc
    39481 caccaggttg ggcaggatct cgcggaagag gatcgcgcga gtaggcaccc cgatgacctt
    39541 ggcggcctgg acgaactcgc gttcgcgcag cgacaggact tcgccgcgga tgaggcgggc
    39601 caggccgatc cagccgaaga acacgaggat gccgatgagc acgacgaagc ggatgctcga
    39661 ggtctcctcg gcggtgagga tctcaccggg ggcagcgaac cgcttctgga tgatcgggac
    39721 cagtgccagc accatgagca ggaacggcag gctgagcagc aggtcgatga tccacgtgat
    39781 caccttgtcg accttgccgc cgaggtagcc ggcggccaga cccatgatga tgccgagaat
    39841 ggtcgagaag gtcgcggcga cgaacccgat gatcaacgag gggcgagccc cgaaggccca
    39901 caccgcgaac aggtcacgcc cagtgcgcgg ctcgacgccg aaccagtgct gcgggttggc
    39961 gccgatgatg ggataggtgt tgagggccga gatgagctcc gggtggtact tgttgagctc
    40021 ttcgccctcc aacgccacca gcaggggcgc gaagatcgcg accaggatga agaagatgac
    40081 gatcaccaac gaggtgatgg cgaccttgtc ccgcttgagc cgatcccagg cgatctgcga
    40141 cggcgaccgg cctgagcgcc tgtcggtcgc tgccgcggcg gtcgcccgag cggccgaatc
    40201 gtccgctact tcggtcgtcg aacccatcgt caaggcgagc gctcgcttcc ttcggtcgtc
    40261 gatcgtgcgg gttggccaac ggtccctggc gcgcaccccc cacaggtcgc tgccagtcgc
    40321 caccgttgtg gctactaccg cgacaccatg tcacacaaaa ccaccacggg gcacgacgct
    40381 gcgcgtcagc gataacaaat cgttacccac cagttctccc cgaccgaaga cggttgtcca
    40441 cgcacgacac gaccgaagat ctcaccagtt ggacagcgaa accacccctc gggatggcag
    40501 ccggtcatcc ccgcagcagg cccttgagcc aggtccatcg agtggccagt cgctcctcga
    40561 atccgcggcc ggtcgggcgg tagtaatcga cccgtccgga caggtcgtcc gggaggtact
    40621 gctgggtcgc caccccctcc ggagcgtcgt gcgcataccg gtagacggcg tccgcggggc
    40681 cggccccggc ggccgcggcc gacgccgcga cccgggcgga accggcatac ccgctccccc
    40741 gcaggtgcaa cggcacggga ccgccgcgcc cggcccgcac gtcggcgatg gccgcgtcga
    40801 tgccgaggta tgccgcgttc gacttcgggg ccaaggtgac gtgcacaacg gcctgggcca
    40861 ggacgatccg cgcctccggc atcccgatct gcgccacggc atgcatcgcc gcgaccgcgg
    40921 tctgcagggc cgtcggatcg gccatcccca cgtcttcgct cgcagcgatg acgatccgcc
    40981 gtgcgacgaa ccgcgggtcc tcccccgcct ccagcatccg ggcgaggtag tgcagggctg
    41041 cgtcgacgtc cgacccccgc atcgacttga tgagcgccga cgcgacgtca tagtgctgat
    41101 cgcctgcgcg gtcgtagcgc accgccgcct gggccatggc ccgctcgacg tgggcgaggg
    41161 tgatggccgt gggcgtatcg cgcgtcggct cggccgcacc gagcggcacc tggtctccgg
    41221 ccacgccggc cgcggcctcc agcgcggtca gcgaacgccg cgcgtcgccg ccggccatcc
    41281 ggacgaggtg tgcggtggcg tcgggatcga gggtgaaggc cccgtccagc cctctggcgt
    41341 cgtcgagcgc cgcgccgacg acggccgcca catcgtcgtc ggacagcggg acaagcgcga
    41401 cgatgaccga gcgcgacagc agcggcgcga cgaccgcgaa gctcgggttc tcggtcgtcg
    41461 cggcgacgag gatgaccgtg cggttctcga cgccaggcag cagggcgtcc tgctgggcct
    41521 tggtgaagcg gtggatctcg tcgaggaaca gcacggtatg ccgtccgtag aggtcgcgct
    41581 ctcgggtggc cgcggccatc acctcacgca cgtccttcac cccggcagtg accgcggaca
    41641 actcaacgaa ccgccgcccc gacgcccccg cgaccaggtg ggccagggtc gtcttgccgg
    41701 tgcccggcgg cccccagaga atcgccgaga gcgggccggc cggcccgctc gacccctcca
    41761 cgagccgccg tagggggctg cccacccgca gcaccgcctc ctggccgcgc acctccgcga
    41821 gactgcgggg tcgcatccgg accgcgaggg gaaggtccag cgccgggtat gccgcgtccg
    41881 ccccggtccc ggcaccggcc ccgaacaggt cacctgacgt cacagaattc ccttcaggtc
    41941 cgctctcgtc cgatgagagg ctatcgctgt gaccgaccgg cgttcgtcta ctcggagggg
    42001 acggcacacc gcgcaggccc cggggtccgc cccgccgcgc cccgccggta gtcatcgcac
    42061 ccagcggcaa ccggggctgt cgcgctgggg agcctcgacc gctgaccatc cgtggcgcgt
    42121 ggtgctcacc tgggcggtcc tggctgtcgc cgccttcctc gtggcagccg ccggcgtggg
    42181 tggtccgtcc ctgttcagca agctgtccac cggggagccc acggcgcccg gtgaagcgac
    42241 gaccggccgt gacctgctgg ccgccgccga gcccgaccga cccgcgatcc tgctcaccgt
    42301 tgacggcgtc gacctggtca gcccggccgt tgcgactgcc gctgcggccg ccacggccga
    42361 catcgcggcc atcgacggag tcgccgacgt cgccagcccg ttcgagttcc ccgccgggac
    42421 cgccgacccg cgcgccgcgg cttttgtcac agccgggtcg accccgccca aggccttcct
    42481 catggtcacg cgcctggagg ccggcctgga gggcgcggcg caggatcgtg ccgaggtcgc
    42541 ggtgcgggcg gcatacaccc ggatgggggg cacgctccca ggggcgagca tcgcgatcgg
    42601 aagcggggcg gccctcggcg acgagatcgt cggccagatg gaggccgatc tgcgcctcgg
    42661 tgagggaatc gctctgcccc tgtccttcgc gctcatggtc gtggtcttcg gcgggttcgt
    42721 cgccgccggg atgccgatca tcggtgcggt cgtctccatc ggcggcgccc tggccgcgct
    42781 gtacggcttc agcactgtca tggacctgga cgccgcgacc gtcaacgtcg tcaccctgct
    42841 cggcctggcc ctgtcgatcg actacgggct gctgttcgtc tcgcgagccc gcgaagagtt
    42901 gcgcgccctg gccgaggggc ggccagccgc agacctcacc cacgagcaga tccggctggc
    42961 gatggccaga actacggcga ccgcagggcg gaccgtgctc ttctccggcg tcaccgtggc
    43021 gatctccctg gccggtctca tggtttttcg gtcgccgacg atccgggcca tcggcgctgc
    43081 ggggatgtcg gtcgtcctcg tggcgatgct ggtcggggtc tcgatgatgc cggcgctgtg
    43141 caccctcgga gcgcggcgcc tgctggcgcg cgggaccgag accgcgccgg acgaaggggt
    43201 cttctcccgc ctggccgcgg ccgttcatcg ccgcccctgg gtggtgatga tcgccacgag
    43261 cgcgctcctg ctggcgatgg ccgtgccggc cctgtcgatg cgactcacct cgtcgggcgt
    43321 cgagctcgtc ccggtcggct cgccgcagcg ggacttctac gagcggctcg accgcgactt
    43381 ccctgcgctg cgggcaccga cggcgaccgt cgtcgtcaac ggcagcctcc agcaagcgca
    43441 ggactgggcc cagggcaccg ttgcccgact ccccggcgtg acccaggcgc aggcgcgtga
    43501 cctgggcgac gggtatgccg cggtcgccgt ccacactggc gctgcgcccc tcgacgaagg
    43561 ctcacgagcc ctcgtcgaga cgctgcgggc gaaccgaccg gccgtcccca tctgggtgac
    43621 cggtcaagcc gcggccctgc tggacctcac cggatcgatc gccgcgggcg cgccgtatgc
    43681 cggcctgctc gtggtgtttg cgaccttcgt gttgctgttc ctcatgaccg ggtcggtcgt
    43741 catcccggtc aaggcgctgg ccctcaacgt cgtgtccctc ggtgcctcgc tgggggcgct
    43801 cacctggatc ttccaggacg ggcacctggc gtcggctctg ggcttcacct ccgtgggcgc
    43861 catcgagtcg atcatcccgc cgctggtcat cgcgttcggc ttcgggctct cgatggacta
    43921 cgagctgttc ctcatctcgc ggatcgccga gctgcaccag gcgggcgtcg agaacaaccg
    43981 tgcggtcgag ctcggcttgc agcgctccgg ccggatcgtc acctcggcgg cggccctggt
    44041 cgtcatcgtg ttcgctggct tcatcgccgg caagatgctc atcatcaagg agatgggcgt
    44101 cgccctcact gtcgccgttc tgctcgacgc caccctggtg cgcatgctgc tggtcccggc
    44161 cacgatggag ttgctcggcg agcggaactg gtgggctccg gcaccgttgg cgcgctggca
    44221 cgcgcgacac ggcatacagg aatgaccgtc aggcggtggg cggcaccggc ttggagtcga
    44281 cgcctgcctc cttgcgctgc tgcggcgtga tcggcgccgg agcgccggtc agcgggtcgt
    44341 agccgccacc ggacttcggg aaggcgatga cgtcgcggat cgactcggcg cccgacagca
    44401 gcgcgcagat gcggtcccag ccgaaggcga tgccgccgtg cggcggggcg ccgaaggcga
    44461 aggcgtccag caggaagtgg aacttctcgc gcgcctcctc ctccgacagg cccatgacgg
    44521 cgaagacccg ctcctggatg tcgcgacggt ggatacggat cgagccgccg ccgatctcgt
    44581 tgccgttgca caccatgtcg taggcatagg cgagcgccga gccggggtcg gagtcgaagg
    44641 tgtcgatgaa ctcccgcttg ggcgaggtga aggcgtggtg gacggcggtc caggcgcccg
    44701 agccgacggc gacatcaccg gcggcgaccg cgtcaccggc cggctcgaac aacggcgcgt
    44761 ccaggaccca caggtggctc cacgagccct cggcgatgag cccgccgcgc ttggcgatct
    44821 cgagccggac cgcgccgagc agggcccggg acgccttggt gacgccggcc gcgaagaaca
    44881 cgcagtcgcc gggggccgca ccgacgtggg cagccagacc ggctcgctcg gcgtcggaga
    44941 ggttcttggc caccgggccg ccgagctcgc cgtcctcctg gaccaggacg taggccaggc
    45001 cgcgagcgcc gcgctgctta gcgaactcct gccaggcgtc gagggtcttg cgcggctggc
    45061 tcgcgccgcc gggcatgacg acagctccga cgtagtcggc ctggaagacg cggaacgcgg
    45121 tgtcggcgaa gtatgccgtg cagtccacga gttcctgtcc gaagcgggtg tcgggcttgt
    45181 cggtcccgaa ccgagccatt gcctcggcgt aggtgagccg cggcagcggg gtcgggatgt
    45241 cgacgccgat gagcttccac agcgcgacgg cgatctcctc accaagcgcg aggatgtcgt
    45301 cctgctcgac gaagctcatc tcgatgtcga gctgggtgaa ctccggctgc cggtcggcgc
    45361 ggaagtcctc gtcccggtaa caacgggcga tctggtagta gcgctccatg ccggcgacca
    45421 tgagcaactg cttgaacagc tgcgggctct gcgggagggc ataccaggac cccggggcca
    45481 gccgggcggg gacgatgaag tcgcgcgcgc cctcgggggt cgagcgggtc atcgtcgggg
    45541 tctcgatctc gacgaagtcg tggccggcaa gcacctcgcg ggcggcagcg ttgaccttgg
    45601 agcgcaaccg gattgccgca ccgggcccag gtcggcgcag gtcgaggtag cggtacttca
    45661 gccgggcctc ctcccccacg gtgacgcgct cgtcgatctg gaacggcagc ggcgcggccg
    45721 cgttgagcac ctcgagggtg gacgcgacga cctcgactgc ccccgtgggc aggtcggcgt
    45781 tggcgttgcc ctcggggcgc agccgcacct caccggtgat ctgcacgcag tactcgttgc
    45841 gcaggtcgtg gccgcctccg tcgtcgagga cctcgtcgcg ggcgaccacc tggacgacgc
    45901 cggacgcgtc ccgcaggtcg aggaacgcca cgccgccgtg atcgcgccgc ttggccaccc
    45961 agcccgcgag ggtgacggtt tggccggagt ggtcggcacg cagggtgccg gcctcatggg
    46021 tgcgaagcac ggtaggtgtc cttctcgggt tgttcggatg gttgcgcagg tatgccgccc
    46081 gctcgccgtt gcggccagcc gacggaccgc gccggggacg gcgcggcggt gaccggtcaa
    46141 tcctaggcgg gcccggcata cctgctcgac cgagttgttc gcccgagttg gtggcgggcc
    46201 cgagtgtggt ttgctgtggg tggccgcggt tcggtgtcac caggagagcc acatgcgtac
    46261 aggggtgcag ggcttgcggg gctggcgacg ggccgccccg gtgactgtcg cggccgtcgg
    46321 gttgctgctc accgcgggtg cgtgtccggc gcccccgatc gagtccgatg cggtggaccg
    46381 agcccgcgtc gatgcgctgc tggccgcgga tccgggcttg gcctccaccg aggtgagcta
    46441 cgggggtggc ggagtgggca gccgcaacct gcgctatctg cgcccgtcga tcaccgtgcg
    46501 aggaaccctc gtgccggccc acccggcggg gtcactggaa ggcctgacgg ccaagcaggt
    46561 acttgccgcc gacgagggca gcgccgtcgc tgccctgcgc agcacgctcg aggacctgcg
    46621 gcgccggggg tgggtcgtga cctccgccaa ctgtgaggtc atcacctcgg tgggcaccag
    46681 cctgcatcgg tcctggctgg tcgaggcccg gcgcccgatg gcgagcgacc cgaaggtctg
    46741 ggcgctgctc cagctcagtg cagccgtctc ccggaactac ggctccgccg acctgtcctt
    46801 tccacgggtg gagatgatcc agcaggcgcg actgcccttc cacctggacg agccgaacgt
    46861 cttcgcgcca ccgagcgggc tccctgctgg tgggctgacc tcgacctgcg ccgatcaggc
    46921 caccttgccg tcggcgaacg cttccgaggg ctcagccttt cccccgctgg tcgacggccc
    46981 ccctcgataa ctccggaccc gagcctcagg tcaccggcaa cacgaggtag tcgatgtgcg
    47041 accagcctcc gaacgcaccc ggggtgcccg tgcccaacat gacactttcg gggacccggg
    47101 tgatcgagcc cgagccgggt tcgtagacgg tgtagtagcc atcggcgtag cccgtgatga
    47161 gcatgacgtg tcgaggggtg tagtcgtcgc cgacgtagaa cggcaccggg gtgccgttgt
    47221 tcgccgcctc cgctgcgcgg ctcatgagct tgcggaccag cgccgggtcc tggtcgtcgt
    47281 acaacaccga ttcgtatgcc gtgggcgagc ccagggtggc cagtgcggct gcgccccacg
    47341 gcggcgtgcc gagtgcttcg ggccacgtgc ccagatcgct gtcggtggtc gtcaacaccg
    47401 aatgctgggc ggcaatgaat cgttcgagga acgtcgcacc gttccactgc gcctcggtga
    47461 ggtgctggag gtaggccggg tcccaccctg ggggccgttg gccggacgtc agccacaggg
    47521 ccagcatcgg atcggaccgg gccagcagag cgagcaggct cgtcgacgca cacgtgttgc
    47581 cggtcgtggg gtcctggatg acgcgatcga ggccgtaggt cgcgccactg gtcaggttgt
    47641 cggtgttgag cgacatcatt cgggcgatgt cctcgtcgct catggaacgg accgcgtcag
    47701 ccatgaagcg cagttgctcc atatcggcac cggcggccag ggtcgacagc agcacggcgc
    47761 gctgcgtcga tgagccggcc gcggccaaca actcgtcgag ggcggcccgt tgggccggcg
    47821 tcagggcggc cagcatggcg cccacacgct gccactggtc agggttgagc aggcgattgt
    47881 cggggtctga ggtacgccat tggtcgacca tgaccgcgac gtccaggacg gtgaagcccg
    47941 gcacgcgagg ggcactcgcc aggaccgccc agccacgccc gtcggagccc tcgcgctgga
    48001 gggcccgcag agcggtctcc gcctgctcat aggcccgcac cgcagtccgc acaccttctc
    48061 gagcgtcggc cagggcgcgg ctcacctccc gggtcaccgc taccgggtcc gggatgtgcg
    48121 tgtcgacacc gtgcacctcg acggtgaggt cggcacagcg acgaaggtgg gtcacggcgt
    48181 cgtcgaagtg gtcatcgacg cggccgtgct ccgtgatcgc gtcctgcagg ctcgcggcat
    48241 accgtcgcag ggcgcgcgcc gagagagcca gcccagtcac ctcgtgactc aggcccgatg
    48301 acaacgcggc ggcagcctgg gtagcggtcc cggccgctac tcccacgacg atcgccggca
    48361 gcccgacgcg gcgccggtcc aggtcggctc ggacctcgtc caccgctcgc ccagccgtgt
    48421 ccagcgacgt cgccatgccc tcgatggtgg cggggtcgcc tgggggcggg tacgagatcg
    48481 tgcgccggaa acgctcgatg tcggcaatga gcgacggtgg ccacacgagg gcgattgcca
    48541 cgtgacccag ggtccgaaga aaactccgag actcgttgag caggtgggtg acctgccact
    48601 ccccgctcac gccgggtcct cgtgcgtcag ggcccccacc accatgacga ggtcggcgaa
    48661 gaaccgccga acccgatggg tgagatccgg cgactgcggt tgtccgtcgg ccaaatcgac
    48721 cgcgagcggt tggagggcgt ccacgaggac cgcgccggcc cgcgggtcgg tcaacagccc
    48781 cggacctagt gcgaggacgg cccgtagccc ggccaactca gcagcccagg cgatctggcg
    48841 aagagccgtc acgtcgacat gggcatcgac caacgcatcg ccgactcggg ccaggtgcag
    48901 ggcctcatcg gtcgaggtcg tcacgccaac cgcattctgt gatcggtccg agccaccgtc
    48961 tcggtctgcg ccctgacggc tgtggcccag acgtccaggg cctcaccccg caggtccagt
    49021 tccgtggccc aggtgtgcag gagttcgctg atcatccccg tcgcgtcgcc ttggttcacg
    49081 gtgatccccg cgagacgcct cgattgggcc tgcacggcat cggccatgga cgccacctgg
    49141 ccgctcgcct cgccggcacc gccctcgtcc cacgtcatcg agcccgtcat cggctccccc
    49201 tgtcacaacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcaccgccct
    49321 cgtcccacgt catcgagccc gtcatcggct ccccctgtca caacctgttc gcgcgatcgg
    49381 tctcgcacgc gtcagccatg accgtaaagc gtccgcgcct cgggcgcctg agtacgtgct
    49441 acttagactc cgggcgactc agatcagacg cgggctactc agactctgag ctactcagac
    49501 tccccgagcc gctgctcaac ccgcctgcgg gccggtgcgg tgaccatccc ggtatgccgg
    49561 gtgctccggc cacggggcat cgtccggtcg cagggtgctc cagccctggg cccgggctcc
    49621 ccaggcggcg aggatgccca gcagcgcgcc ggggttgtgc agctgcccgg ccagcgccgc
    49681 ggcatacgca tcgtcgaggc tgacccagcg gacggtgagg tcggcctcct cgccggtgcg
    49741 cacgtgcggt tcgcccggag cgggagcgag gtcacgggcg aggaacacgc ggtaggcctc
    49801 ggggaagact cccgccgagg cgtaggcgtc gatgagcagg tcccaccggc ccgcgaccag
    49861 gtcgacctcc tcgcgcagct cgcgttcggc ggcgtcgcgc ggcggctccc cggggatgtc
    49921 gagcaggcca gccggcaact cccagaggta ggaaccgcag gcgtggcggt actgccgaat
    49981 catgacgatg tggtcgaccc cgtcgatctc gcgcagcgcg aggatgagca cggcgccggg
    50041 gtggtcggcg tagtcgcggg tgacaacctc ggcgccaagg tcgacccgat cccggcggac
    50101 gtcgaagacc agcccctgcc agacgacgtc gctcgccacg acgggcttcg gcgcgatctc
    50161 gtcccgcaac agcgcggcag tgccgatcgg cgcgtcgaag gccacctcag ccgaccgcgg
    50221 cggtctgtga ctcgggtcgg gtggtgcgct cgacctcgac gaggcgttgc gcgcgctggc
    50281 gatccagggc cgcaccgacc aacccggcga agagcgggtg ggcgcggttg gggcgcgaga
    50341 ggaactccgg gtgcgcctgg gtggcgacga agtaggggtg cacgtcggcc ggcaactcga
    50401 cgaactcaac cagcgacccg tccggggaca gcccggagaa caccaggccg gcgccttcga
    50461 gtcgctcccg gtaggagttg ttgacctcgt agcggtgccg gtggcgttca ctcacctcgg
    50521 tggtgccgta tgccgtggcg acgacactct ccggggtgag gcgggcggca taccggccca
    50581 acctcatcgt gccgccgagg tctccggcgc cttcgacgaa cgccagttgc tcagccatcg
    50641 tcgcaacgac ggggtgggtc gtctgcgggt cgaactcgct ggaggaggcg tcggcgatgc
    50701 ccagcacggt gcgggcgtat tcgatgacca tgcactgcag ccccaggcag atgccgaggg
    50761 tggggacctg acgctcccgt gcccagcgca gggcgccgag cttcccctcg atgccgcgca
    50821 cgccgaaccc accgggcacg aggatcgcgt cgacgccgcc cagggcccgc tgagcgccgg
    50881 cctcggtctg gcactcgtcg ctggccaccc agcggatgac gaccttggcg tcgtggtgga
    50941 aaccacccgc acgcaacgcc tcggtcaccg acaggtaggc gtcggggagg tcgatgtact
    51001 tgccgacgag cgcgatctcg atctggtgct cggggtcgtg gacccgcttg agcatctcgt
    51061 tccacgaggt ccagttgacg tcgcggaagc ccaggcccag gcggcggatg acgtaggcgt
    51121 ccaggccctc accgtgcagg accttgggga tgtcgtagat cgacggtgca tcgacggccg
    51181 cggcgaccgc gtcctggtcg acgtcacaca tgagcgagat cttgcgcttg atcggctcgg
    51241 ggatctcccg gtcggcccgc aggacgagcc cgtcgggctg gatgccgacc tggcgcaggg
    51301 ccgccacgga gtgctgcgtc ggcttggtct tcagctcgcc actcggggcg aggtagggca
    51361 ccagcgagac gtgcaggaag aagcagttgt cccggccgat ctcctggcgc acctgacgcg
    51421 cggcctcaag gaacggcagg ctctcgatgt caccgacggt gccgccgatc tcggtgatga
    51481 tgaggtcggg cgcgtccttg tcaccggtcg ccaacgcccg catccgggcc ttgatctcgt
    51541 tggtgatgtg cgggat
//
LOCUS       DAORPW010000003        51386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_79_length_51386_cov_634.274006, whole genome shotgun sequence.
ACCESSION   DAORPW010000003 DAORPW010000000
VERSION     DAORPW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 51386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51386
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79_length_51386_cov_634.274006"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLB86_00570"
     CDS             complement(<1..353)
                     /locus_tag="PLB86_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRC63658.1"
                     /translation="MRSDRLGETLLPKKLALPIFASDALSSVAYAPDEILIMLGLAGG
                     AFAAMHSWQITLGVIFVMVIVVASYRQNVHAYPSGGGDYEVATENLGPKAGLTVASAL
                     LVDYVLTVAVSISSGV"
     gene            490..1152
                     /locus_tag="PLB86_00575"
     CDS             490..1152
                     /locus_tag="PLB86_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRC63659.1"
                     /translation="MHFVIMGCGRVGATLARSLSDLGHDVAVIDMDPTSFRRLGSSFE
                     GRTITGMGFDRGVLVAAGIREAYAFAAVSSGDNSNILAARVARETFGVEHVAARIYDP
                     RRAAVYQRLGIPTVATVRWTADQIIHRLLPTGSVPAFTDPSGQVVLAEVPVAAAWVGR
                     RVTTIERLASTRVAFLTRLGEGMVPDADTVLQDGDLMHVCAPATELDRLERVFAARPP
                     HE"
     gene            1189..>1865
                     /locus_tag="PLB86_00580"
     CDS             1189..>1865
                     /locus_tag="PLB86_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRC63660.1"
                     /translation="MRVAIVGAGSVGRSIARELLHNGHQVLLIDRDADDVVASRVPDA
                     TWLLADACEISALEEAHLEACDVVVAATGDDKANLVVSLLAKTEFGVPRTVARVNNPR
                     NEWMFDEAWGVDVAVSTPRLMTALVEEAVSIGDLVRVFEFRQAKSAMVEMTLPPTSPY
                     AGARVGDIPWPEDTVLVGIIREGRPIAPSRDDALEPQDELLFIAARTMEDNLENLLSP
                     GHLPRGK"
     assembly_gap    1866..1965
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1966..2639)
                     /locus_tag="PLB86_00585"
     CDS             complement(<1966..2639)
                     /locus_tag="PLB86_00585"
                     /inference="COORDINATES: protein motif:HMM:NF022805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HRC63661.1"
                     /translation="MADQTDAGRPAAQESFVTVEEVLRARLGTVFGGWRGAVESAVPT
                     VLFVVVWNVTSDIPLAALSALAAAVVIAGIRLAQGQTLRYVGWAVIAVVISGVVATRT
                     GRAQDAFLPGMLQTGFSLLVFTISNLVRWPVIGFLIAAGDPELMQTSARVRFAGSREG
                     RAAMARMADEQRAAAEQAAEADAQAMSHAFTSWRRHDGIVKVASRLGWVLVGLAVLRL
                     SIQIPL"
     gene            complement(2632..3030)
                     /locus_tag="PLB86_00590"
     CDS             complement(2632..3030)
                     /locus_tag="PLB86_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRC63662.1"
                     /translation="MSDEGSPGRSESSTWSRLMGRLTKSSAELQADELHDASLRLGAT
                     PISALVPRQRVTVSGEVRSVALRPQVQVPALVAEVFDGTGTLILVWLGRRAVAGIVPG
                     VMLRIHGRVTDLRAGPTIYNPAYEILPQHG"
     gene            complement(3033..3809)
                     /locus_tag="PLB86_00595"
     CDS             complement(3033..3809)
                     /locus_tag="PLB86_00595"
                     /inference="COORDINATES: protein motif:HMM:NF023917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HRC63663.1"
                     /translation="MGLFGRKRAKDETERPVAETGADGIPASLPSDAEATAATVAESD
                     DWEPETPFSRLRGPFDRAEVTRDDTRVELGSLWVAITDGVQVALQVDESTQTPVGIQL
                     SLGTSGAYLQAYAAPRTEGIWPEIRTEVAQSVVAAGGRAELVSGPLGKELRLHQRGGV
                     PIRLLGVDGPRWFLRALITGPAAVDEQAGDALIEVIRKTVVDRGTQAMAPREQLPLRL
                     PQADGPEAARPDASPGGEPTPAGFDDLQPFERGPEITEVH"
     gene            complement(3863..4312)
                     /gene="dut"
                     /locus_tag="PLB86_00600"
     CDS             complement(3863..4312)
                     /gene="dut"
                     /locus_tag="PLB86_00600"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRC63664.1"
                     /translation="MPGVPVQVHRLDPDLPLPAYAHPGDAGIDLFARVDVLLAPGQRA
                     LVPTGIAIALPEGYAAFGHPRSGLAIRHGITILNAPGTIDSGYRGEIFVNLVNLDASE
                     SFQVSRGDRIAQLVVQRVEAVQWVEVDSLPDSPRGDTGHGASGGFGA"
     gene            complement(4312..4611)
                     /locus_tag="PLB86_00605"
     CDS             complement(4312..4611)
                     /locus_tag="PLB86_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HRC63665.1"
                     /translation="MATDYDAPRKTDDDLSEDSLEELKARRVDKASSTVDVDETEQAE
                     GFELPGADLSGEELSYRVIPRQADEFTCSRCFLVHHRSQLAKNVGGQMVCRECVA"
     gene            complement(4851..5750)
                     /locus_tag="PLB86_00610"
     CDS             complement(4851..5750)
                     /locus_tag="PLB86_00610"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRC63666.1"
                     /translation="MTPIPDTTGHLPVPSIDDLSVLEALALTLAHETGQLVHEGRPAD
                     LANTTGTKTSDTDPVTVMDTRAEEHLRRRLRAARPDDGLQGEEGSSIPSRSGLTWVVD
                     PIDGTTNYLYDLPLYAVSVAVVVGDPTTPGAWRPVAGAVRAPALDITWSARAGGGAWR
                     RGPARPWDVAGPGGEPPAVPARVGRQSHLGPALVGTGFGYQPERRAQQAEVLTRVLPA
                     VRDLRRMGSAAIDLCLVGDGRLDGFFEVGLNPWDLAAGWLVVTEAGGSVSGVGGGAPG
                     VDLVVAANPALHPRLLALLESPA"
     gene            5851..>6656
                     /gene="sepH"
                     /locus_tag="PLB86_00615"
     CDS             5851..>6656
                     /gene="sepH"
                     /locus_tag="PLB86_00615"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HRC63667.1"
                     /translation="MQDLRLIGVDDDGGHLLLASVDGARFRVPLDESLRAAARRDRPR
                     LGQLQIEIEGGMRPRDVQAMVRAGMSADEVAERSGWTVEKVHKYEGPILAEREYVAGL
                     GRAVRLRQRGGSGPSTTLGQRVADRLTARGVEADSIEWDAWRREGPEWTVAVNFSAGG
                     RVRQGTWAFDVASRSLHAKDDEARWLSEDEPAATPVPREAPVYDVEADGGLDAAGRRS
                     RADDPVDLVTAMRERSAARSRRGSRRRGLEPVAALPLGDAVQSPPDDHVDT"
     assembly_gap    6657..6756
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6757..7163
                     /locus_tag="PLB86_00620"
     CDS             <6757..7163
                     /locus_tag="PLB86_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63668.1"
                     /translation="ALPLGDAVQSPPDDHVDTVETIPSDVAAAEERRPVAVTAAVTRE
                     AEPELAIAPGPDSITESSTAASTDSNTEPDSEPKHEPAFEPRQDPKPEVEVEEVSPEP
                     AVARHTPRRSGRPSVPSWDDIVFGTKPKDPNA"
     gene            complement(7186..7848)
                     /locus_tag="PLB86_00625"
     CDS             complement(7186..7848)
                     /locus_tag="PLB86_00625"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRC63669.1"
                     /translation="MAELVFFSGTMDSGKSTLALQMEHNHRARGKGGLIFTKHDRAGE
                     AVVSSRLGLSRRAIEVTDTLDFWDTVVGFVTEGHRVDFVVCDEAQFYTPRQIEQLARI
                     VDEMGVDVFAFGIMADFRTELFPGSRRLVELADRVQVLQVEALCWCGVRATHNARVID
                     GEMVIEGDQVVVGDTAKGSGVVEYEVLCRRHYMRRMTSHAARAAALSPDVLPFDVARQ
                     DG"
     gene            complement(7860..9023)
                     /locus_tag="PLB86_00630"
     CDS             complement(7860..9023)
                     /locus_tag="PLB86_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRC63670.1"
                     /translation="MGSAPPALPVGAGTLSAVLPSVLASLGSPRYAARPLIPLTPARR
                     AVVVLVDGLGDTLVRRRAGHAPFLRSGLAAAYRLTCGFPSTTATSMGSFGTGLPPGAH
                     GLVGYEVLVPGATPETDRILNELSWDDGPDPFTWQPEPTVFEAAEAEGVAVTRVGPGF
                     FDGSGLTNAALRGGRFRAAYQLATRVDEALAAVRATPRALVYLYWGDVDKLGHVHGPS
                     SPEWGAEVERVDAELRRLVASVPSDTAVYVTADHGMVEVPFADRIDLAVEPRLLDGVR
                     HVGGEPRSVQLYCAPGAAQDVSETWRERLGENATVISRSEAIEAGLFGPAGSVADRVL
                     PRIGDVLVNCLGSMAVVDSVRMRPELIALLGLHGSVSEDEIAIPLFQWPARTA"
     gene            complement(9025..9615)
                     /locus_tag="PLB86_00635"
     CDS             complement(9025..9615)
                     /locus_tag="PLB86_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HRC63671.1"
                     /translation="MRKVPREVPGFSLPVELRSDIQHAGYYPALVADVVGATLAGDVV
                     QAHLVHQETTFDQDSVRRHITVLVLTATKLVVVHADDHDDHHERAVATATSESIPLAA
                     VRGVMLAHVVANPDAYVPGSLGREITLTLGWGAVSRLDVMPASCGDPQCEADHGFEGT
                     LAADDISLRISADADGDAALARALHFASALSATIGR"
     gene            complement(9646..12375)
                     /locus_tag="PLB86_00640"
     CDS             complement(9646..12375)
                     /locus_tag="PLB86_00640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC63672.1"
                     /translation="MSSKHGQHRHPLPDEPTSPEPVGGGDEHPPGYPFEWEADVVLSD
                     GSVAHVRPIRPSDDERIRVFHAGQSEESIYLRFFAPLRELSDRDVQRFTVVDYDERVA
                     LVVMHRDLIIGIGRYDKIDDTRAEVAFNVSDAFQGKGIGSILLEHLALVGLDGGVTRF
                     VAEVLPQNRRMINVFKEAGYNVKHRLEDGVISLSFDISPTATSTAVRYAREHRSEAIS
                     VGGILTPRSVAVVGASRREFSIGHTFLKNLVEGGFTGQVYAVNPNAKRVMGLKSYRTL
                     ADVPGLVDLVVVAVAAPDVINLVDECAAKGARAIVVPSAHFAEDGEQGWKLQTKLRKK
                     ARRAGLRVIGPNSFGIINNHPDVRLNASLAPQLPPRGSFGLFAQSGALGIAVLASAAR
                     RGLGVSVFASAGNRVDLSGNDLMQYWIEDASTDAVGLYVESIGNPRKFVRIARSLAAA
                     KPVIVVKSAGSRLGSAPGHLTRKMSVGTEAFDALLRQSGVIRTENIHQLFDVAQLVVH
                     QELPAGDRVAVVTNSDALGSLTTQAAQSWDLKVTHGPVSVAPEAGAEEFALALRAAFD
                     DPGVDSVIASFIPPIYTDDELVAAAVRDEAGRSTKTCTATFLGMRGVTEQLTLNTPGE
                     RPRVVPAYPMPEDAVRAVAAATRYAQWRARDKGTLVAPSGIEKGVVEELIDNVLTDSP
                     DGRALTEDECIQVLAAYGIGLWPADLVHNADEAVEAAERLGYPVILKVIAPALRHQVG
                     VTGVRPDLSSPEAVRDAHASLTNRLGAFVADRFAVQRMSPPGVSCVVGSTEDPLFGPV
                     VSFSIAGPPTDLLGDIGYRIPPLTNVDVDDLIFSVKAAPLLTGHGGKEPIYLDDLRDL
                     IARVSTLADNHSTLASVSLQPVNCWGAGVDVLGAEIFVAPAMTRKDASRRAMT"
     gene            12437..13054
                     /locus_tag="PLB86_00645"
     CDS             12437..13054
                     /locus_tag="PLB86_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRC63673.1"
                     /translation="MELSRAIRGRRMIRRYDPDRPVPDEAVLACLGAAIRAPSAGFSQ
                     GWDFLVLRSPEERATFWSATTGDQPPDTWLAGMATAPALILCLSDPERYLERYAQPDK
                     GWTDRDPARWPVPYWDVDTGMAAMLALLTAVDAGLGACFFGVPPTRHTEVRSALGIPD
                     DRRFVGVISLGYAVPHPASPSLARGRRGLDEVAHWGRFGVHDPLP"
     gene            13301..15781
                     /locus_tag="PLB86_00650"
     CDS             13301..15781
                     /locus_tag="PLB86_00650"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016664672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HRC63674.1"
                     /translation="MARRTTTQPPDGEDVVERIVDIDVAEEMKAAFLEYSYSVIYARA
                     LPDARDGLKPVQRRILFGMSELGLRPERPHVKSSRIVGEVMGKYHPHGDSAIYDTLVR
                     LAQPFSMRLPLVDGHGNFGSLDDGPAASRYTEARMAPGAMLMVAALDEDTVDFVPNYD
                     DTQTQPEVLPAAFPNLLVNGASGIAVGMATSMPPHNLGEVIGAARHLIEHPDATLDDL
                     MRFVPGPDLPGGGRIVGLDGIREAYAGGRGTFRTRATARIENITPRRRGIVVTELPYL
                     VGPEKVIEKIRDQVQSKRLLGISDVKDLTDRSNGLQLVIEVKSGFVPEAVLEELYRLT
                     PMEDSFGINNVALVEGQPRTLGLVELLSVFVDFRIGVVRRRTAHRLAKREERLHLVDG
                     LVVAILNIDEVIQLIRAADDTATARARLMGVFDLSEAQATYILELQLRRLTKFSRLEL
                     EKEQEQLRSEIEALRAVLSDERLLRRTVSDELAEVAQAHATPRRTVLLESAGAVAGVT
                     AAAPARGQSAVLEIADDPCWVLLSSTGLLARTTSADDLPTGGGRARHDVIIGAVRATA
                     RGEVGLVTSLGRMLRLSVLELPTLPPTADSPHLSGGAPLAAYLELGRDEQPVTLASLA
                     VDSPVMALGTASGVVKRVVTDHPARPSWELIALRDGDQVVGAAPTSSDEIDLVFLTSD
                     AQLLRFSAGSVRPQGRAAAGMAGIRLDPAARALFFGVVDPTRDGLVVTASGTSGALPG
                     TQAGAIKVTPYAEYPAKGRGTGGVRAHRFLKGEDTLVLAWAGSVPARAAAANGGPVDL
                     PAATGRRDGSGATWSHVVAAIAGPASGA"
     gene            16141..17469
                     /locus_tag="PLB86_00655"
     CDS             16141..17469
                     /locus_tag="PLB86_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRC63675.1"
                     /translation="MRRSTFAKAATGAAAIALLTSGCLGSSGTTTTAATGTAAGAGKA
                     TVEVMYGFGNEQETAFQKDLKAFADKSGFTIKFTKAGSWDTEIKTRVAGGNPPDVGLF
                     PQPGVMCDLAKQGKVLAYDDATVTSAKATLVNGFVGAGTCTDGKVYGLPSAVSVKSLL
                     WYDAPVFAAAGYKAPTTLAELDALTEKIKADGKTPWCIAAESGGATGWPITDWIEDLV
                     LRFAGPENYDKWVAGTLKFNSPEIKPAWDYFQKIAFTEGNVRGGTKAIAATNFQTGGN
                     VLFDTPPACHLFKQATFIAGKGGFPDTVLAKADTEIGVVAFPAKEAGNNPVLTGGDLA
                     TAFNNDANTLKLRNFIASKDNGVEVGKAGYFSPHKSFDVTLYPSKMLQKIASDVLYKS
                     SAARFDGSDLMPAKVGAGTFWTEPIKWISGTQDLDVTLNNIDASWPKAAS"
     gene            17566..18549
                     /locus_tag="PLB86_00660"
     CDS             17566..18549
                     /locus_tag="PLB86_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRC63676.1"
                     /translation="MTIKILNALLAIIGGIGGAMILFWLLNKLAESLKGRWEDRVKPW
                     VFAGPAILAIGVYLIYPAIVTLQYSFANEDATKYVGFNNYKDLLTDATFLSVLFNNLL
                     WIIIVPALTVALGLGVAVLADRLRPKGEKLSKTVIFLPMAISMVGAATIWRSIYEYQP
                     KGSPQTGLLNAIMGVFGKDPVYWYQIETWHLNSLLLMVILIWTQVGYSMVLLSSAIKG
                     VPEDTIEAGRIDGAGERRIFFSIVVPQIWPTVVTVFITVLIGVMKVFDVVYVTTNGAF
                     NTDVVGRRFFYEMFTNQNSGYAAAIVVLLMIAITPVLVYQVRQFRAEEANR"
     gene            18546..19463
                     /locus_tag="PLB86_00665"
     CDS             18546..19463
                     /locus_tag="PLB86_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRC63677.1"
                     /translation="MTTAVPPSTDAQVIRQSKAGSGPGSATWPVRIFVTFLCLIWLIP
                     VLGLLISSFRTRDAQFNSGWWTAIANPLEFTQWTVNNYAQVLTTNDSGINMGQAFINS
                     FIVTVPATVIPILIAAFAAYAFTFMEWKGRDLVFVIIVGLLVVPNQVALVPLLKLYSS
                     LGLNGTFLAVWLAHIGFGMPLAVYILRNYMSSLPKAVIESAKIDGASHFTTFWRLIIP
                     MSTPALASFAIFQFLWVWNDLLIALLFLGRGENTIATVALQGLQGQTGQGRELIPSAG
                     FITIIVPLMVFLSLQRYFIRGMTSGAVKG"
     gene            19546..21165
                     /locus_tag="PLB86_00670"
     CDS             19546..21165
                     /locus_tag="PLB86_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 66 protein"
                     /protein_id="HRC63678.1"
                     /translation="MTTPSHPTVLPTRSLFRATDRVAVEIRDLRQSAELTVHRDGAVL
                     SSHPVSSAGVIPLGELEPGSYGISLRAGDQQIARSAVQIAGSGGDRGILRYAFVADYR
                     PDRSVADLAAVADNLRRLHITGVQFYDWAYRHADLLGGGERYADALGSPVDLGTVRAL
                     IATCHEVGADALGYAAVYGVGNDEWPAWESLAITDADGTAYGLGDFLRLVDPGAPTWL
                     YHFGNDLAEALAQCGFDGFHLDQYGYPKVALRTDGTRVDLTESFVHLIESVRERVPGA
                     RLVFNNVNDFPTWASAQAPQDAVYIEVWEPHSTHAALAQVIGRARSVAGGRPVVIAAY
                     QQVYAVAPREEADRATALTMATVFSHGATHLLAGEADRILVDPYYVRNHVAQPGTMDL
                     LARYYDFLTEHHDLLIPSGIVEVTGSYAASYNDDLDVSYAAATVSGTARPGVVWRRIT
                     AVGDALVVHLINLSGATDCRWDAPQGPPGTPGAGTLRMRRVGPDVPAVYAADPDASGH
                     LEPLVVAVDGTHATVHLPPLATWQMIVVRSR"
     gene            21209..22162
                     /locus_tag="PLB86_00675"
     CDS             21209..22162
                     /locus_tag="PLB86_00675"
                     /inference="COORDINATES: protein motif:HMM:NF018677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HRC63679.1"
                     /translation="MTIVDPACSRVWDTGALPAWGTAARADFVVALEQNGPWGRVAAT
                     QSHLDPALGAALDAHVSGLGGRLLLIRRPGRHAEQAPQTDAEPEARGAGAGAGADGAR
                     PQQSTRRTYIARTGPGGWLVRADLTDPAMLLGLGADELASAGATAGRLGGALDTVPLL
                     LVCTNGRRDACCAQRGRVVTSAFVSGGFATAAPDQVWETSHTGGHRFAPTAVLLPWGR
                     VLARLDPELAAGALDAAASGRLAPALLGRVHDRGPMHLPPAEQAADAWLRRQTDRRDL
                     DDPMDDLREQVDVVARQGPMLPESCGKAAVPTIVYAPSWRA"
     gene            22272..24467
                     /locus_tag="PLB86_00680"
     CDS             22272..24467
                     /locus_tag="PLB86_00680"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRC63680.1"
                     /translation="MTTAPSVPTMLHLRLGGTSVVARLDDAALPCVLHWGPDLGDVST
                     QALDELQIALTPPYVDSAISSQAAVALLPQHSSGWLGRPGLLGSRAGGRSWSVSFDRV
                     EHRVETAGSADWADGAAGAVRLRSVGLDRPSALEVTTEIEVHPSGLVRVRAQVRNLGA
                     DDYEVAHLLPALPVPAEAGELLDMTGRHTHERTPQRRPFDPGAWVREAWGGRPGHDSA
                     TVMCAGEPGFGFRSGRVWGVHVAHSGNQVVLAEHSTAGWRLLRGGEVLLPGEGVLHPQ
                     ESYSSPWLVASWGEGLDAFSQRVHRFLRARPHHPRGPRPVLLNTWEAVYFDHDLGKLI
                     TLADRAAELGIERFVLDDGWFRHRRSDQAGLGDWFVDEGVWPDGLHPLVDHVHGLGMD
                     FGLWFEPEMINLDSDLAREHPDWVFQTDHGPGLPSRYQHVLDLGHPAAYAFVLERMST
                     LVGEYRIAFIKWDHNRPLVDAGHSPDGSPGVHIHTEAVYRLMAELKARHPGLEIESCC
                     GGGGRVDLGIIEHTDRVWVSDCIDAHERQRLVRWTGLLLPPELLGTHVGSGADHSTHR
                     LLHLGFRAGTAMWGHLGVEWDLTTASSADLDTLAAWIAFHKEVRELLHTGDVVHADLP
                     NPALLLEGVVATDRSDALYRLAALDLTVTWPAGRVRLPGLDPARDYHVSAQAPGDAAA
                     ATFPPGWGPSGVTLPGRVLREVGIQAPLLNPDQLVLLRARALPTAAVPV"
     gene            24684..25706
                     /locus_tag="PLB86_00685"
     CDS             24684..25706
                     /locus_tag="PLB86_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRC63681.1"
                     /translation="MQQPTIADVARAAGVSVATVSRALRGLDKVHPETRERVVQAAAQ
                     LDYIASPTASGLASGRSRLIGIITPFMARWFFTGIMSAIEKTLREHQHHILLMDLERS
                     ETSMSRLSLTQGMLFKRVDGLIVINVELQDAERDLVRRLGLPVVAVGSPFEDSPCVGI
                     DDIGSAALAAEHLLALGHRKIAYVGRDYPEAAPRKTPIDRLEGFHQAMSRAGINTPPT
                     WVLDSDWTAGDSRASAMALLSGPDRPTAVLAASDEMALGVWGAARQLGLEVPRDLSIV
                     GIDDHELAPVFGLTTVRQDVAAQGVAAASTLLALLGLYDPSAIPDQTFPVELVIRGST
                     TAPAKP"
     gene            complement(25762..26538)
                     /locus_tag="PLB86_00690"
     CDS             complement(25762..26538)
                     /locus_tag="PLB86_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63682.1"
                     /translation="MTLAPHPAPARPAALDPDEPAAARHARLAMRRRLISWSIGPAFV
                     AILVAVKLLTMSAAATQAVDGFFAAAAHTVFEAADRMSFLNVVERHKAPFARGDAFVL
                     AGDLDSARAQFEQALELAPPDSIDSCQIRVNLALALEKLGDAAKAAGDEVAAKDYWTR
                     TKAVADAAPPGCFPPAADGAGQKAEAAGDRAEQKLNPQQSSGVDQSSDAQQQAEQQRK
                     ADELDQQTHENQQDRQQNGPDGQGNPAGGGGSANPAAKPW"
     gene            complement(26535..27599)
                     /locus_tag="PLB86_00695"
     CDS             complement(26535..27599)
                     /locus_tag="PLB86_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC63683.1"
                     /translation="MTLLPVMSVGVLVLAVVVALALVWWPQPGPQPSLRTRVRRTLLV
                     AAVLVASLRPGLPGAEVRASASELNVFFVVDTTTSSMAEDFGTDPRMSGMRADITAIS
                     GRMAGARFALITFDQAAVLRMPLTSDGAAVAAAAETMLPETSAWSRGSSVTAAGPLLQ
                     QTLARVAAAHPERARAVFYLGDGEQTSGSPPAALGVDQSLVNGGAVLGYGTTGGGQMH
                     ETGVPGSRADAGWLLDPSTGKPAVSVIDEGQLGSIAQQLGVPYLHRSPGESVDAVLGL
                     VDLTGLDRVTVAQDGPLSAGREEFYWIALLAVAGLVAWEVGLALARLWGLRRPRRARG
                     IPEDGGIPADRDGHEEKVRS"
     gene            complement(27596..28567)
                     /locus_tag="PLB86_00700"
     CDS             complement(27596..28567)
                     /locus_tag="PLB86_00700"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC63684.1"
                     /translation="MALKQPWILPWAALGALAIAALVWFALRRRRPPAALPLANTDLL
                     TSLPEYVHAIWRHRLMTVLYAAAAGFVAVLAIIGAARPVATTVINPEKANRDIVLCLD
                     VSGSMTTADAEVAETFRSLAKEFKGERLSLVIFNSSAVPVFPLTDDYDFVEEQLSDAQ
                     RVFEGSFDHEFYAGTLNGQGSSLIGDGLATCVRSFDHGNLTRARSIILATDNLAAGRS
                     IFSLAQGGTMAKAAGIQIYGLNPAHRAGSSTATEMRSVAEGSGGLYYPLNSVDAVSRI
                     VSAVSAREASRIPGAPITLVHDQPASWVGWTWLGVLGLLGALRRWRR"
     gene            complement(28558..29040)
                     /locus_tag="PLB86_00705"
     CDS             complement(28558..29040)
                     /locus_tag="PLB86_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63685.1"
                     /translation="MHVDDPFYPPMTYHWVWTLLAWVAILAVLAWAGFIWWRTRRVRP
                     TTPAGPVPIHPGWRLARAKHDTMTRIDQIVWECEQGRVDARTSHRELSAAVRAFIDDV
                     SGATTSRMTLSDLGSRGPGLAPVTHVVLQLYPGEFGPDPARPIRPAAEAAKAVVAQWH
                     "
     gene            complement(29030..29914)
                     /locus_tag="PLB86_00710"
     CDS             complement(29030..29914)
                     /locus_tag="PLB86_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018769444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC63686.1"
                     /translation="MAALLTRVKSKLFIVAHRRTWGLLDGEYASVFRGRSLDYDDLRE
                     YIPGDEIRDIDWKATARHGAPLVKRYVATRKQTVLLLVDTGRSMAAMAPSGEPKKDIA
                     IQIAGMLGYLAVRHGDLVGLISGDGESTRMLEPAAGEANLERLLRVIDRASTLEAPPS
                     RFSAQLHYLAEHVRRRMLIVAVTDDGGLGSDDDPLLRRLHAQHEVLWVVVADADPTRF
                     DPRADASEVTDGSALPGAVRGGERLQAAYQQALAGRREATDQRLRRLGIARARVGGTS
                     EVVGSMFALLERQRRRAR"
     gene            complement(29967..31034)
                     /locus_tag="PLB86_00715"
     CDS             complement(29967..31034)
                     /locus_tag="PLB86_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC63687.1"
                     /translation="MSTTIDPGPPPGAQAAYPTSASTTPRPGVTAIPPEDLAWARSTV
                     ATISRSFQERVVGQVRLHETLLLGLLTGGHILLESVPGLAKTTAAATLARTVGGSFKR
                     IQCTPDLLPSDIVGTQVYDVRTTQFETRLGPVHANLVLLDEINRSSAKTQSAMLEAMQ
                     ERQTSIGGQVYPLPEPFLVLATQNPIEQEGTYVLPEAQLDRFMLKDVLDYPTLDEEAE
                     VVRRIDAGIYLAEQTPAVSLADVRRLQALTRAVYLDDTVIRYAVGLAYVTRHAERYLD
                     PALARYVEFGASPRASIAFAQAARARALLAGRDHAVPDDVQALAHRVLRHRIILGFEA
                     VADDVPVERVIDAIITAVRTP"
     gene            complement(31168..33333)
                     /locus_tag="PLB86_00720"
     CDS             complement(31168..33333)
                     /locus_tag="PLB86_00720"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRC63688.1"
                     /translation="MATRSTTGRTAASGSGAGGGDYTARHLQVLEGLEAVRKRPGMYI
                     GSTDQRGLMHCLWEIIDNAVDEALAGVGDRIEIILGGDGSIEVRDNGRGIPVDTEPRS
                     GLSGVELVFTKLHAGGKFGGGSYAASGGLHGVGASVVNALSSRLDVEVDRGGKTYAMS
                     FRRGEPGVFADPPGPHSPEAPFTAYETGSELRVVGKAPRGATGSRIRFWPDLQIFLKE
                     ARLDDDLLAARARQTSFLVPGLALVVRDERGAPDTPGAQGTHEQVFQHLGGISEFAEF
                     LAPDPPLTAVWRLQGTGNFTETVPVLDERGHMSPTEVERECSVDIAMRWGTGYDSRVS
                     SFVNIIATPKGGTHVAGFEQSLLKVFRRQLELNARRLKVGTDKVEKDDILAGLTAVVT
                     VRLAEPQFEGQTKEVLGTAAVRAVVAKVLEQELNARLTSTKRDDKAHAALLLEKVVSE
                     MKARISARQHKETQRRKTALESSTLPAKLADCRTHDVLRSELFIVEGDSALGTAKLAR
                     SSDYQALLPIRGKILNVQKASVADVLKNTECAAIIQVIGAGSGRSFDLEAARYGKVII
                     MSDADVDGAHIRTLLLTLFFKYMRPLVAAGRVYAAMPPLHRLEVIGGPGRKNEYVYTY
                     NEAQMRSTRAALERKGRKVKEPPQRYKGLGEMDADQLADTTMDPRFRTLRRVTLGEAE
                     EAARIAEGVFDLLMGNDVAPRKDFIVESAKTLDRERIDA"
     gene            33597..33818
                     /locus_tag="PLB86_00725"
     CDS             33597..33818
                     /locus_tag="PLB86_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63689.1"
                     /translation="MNATLVHRLSAADRCDRCGARAYIRARLHSGGELYFCAHHGRQH
                     LPQLADQAVEIHDETELLTETADESTGLR"
     gene            33815..34318
                     /locus_tag="PLB86_00730"
     CDS             33815..34318
                     /locus_tag="PLB86_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRC63690.1"
                     /translation="MSFADPVSLTIVTGLLMVVGLVGIVVPVVPGLLLTVAATLLWAY
                     AHPAPGAWVVFWVAVVLYAAGVVTQYLLPGRKMRREGVGTSTMLLAVLLGIVGFFVIP
                     VVGALVGFVLGIFLVELTRRQDRSAAWTSTKAALVAVLHSMGIELLAGLAILLTWIIG
                     LIVLGPG"
     gene            complement(34370..34762)
                     /locus_tag="PLB86_00735"
     CDS             complement(34370..34762)
                     /locus_tag="PLB86_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HRC63691.1"
                     /translation="MALSEREQKLLEQMEQALYAEDPRFATRIRSHGDRGTKRRMLVG
                     ILAVLAGLGLVFLGVLGQAIWLGAVGFAVMVAGGVWAFAPARKTGPVGLVQGDGSTAA
                     RTAKARKSGQGFMNMLEERWERRDRDQW"
     gene            complement(34932..36191)
                     /gene="dinB"
                     /locus_tag="PLB86_00740"
     CDS             complement(34932..36191)
                     /gene="dinB"
                     /locus_tag="PLB86_00740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRC63692.1"
                     /translation="MSRRQFRPPPRTGDGPPDDTGCTVLHVDMDAFYASASLIDRPEL
                     RGTPVIIGGGGRSVVLSATYEARAFGVTSAMPMTRARRLCPQATVIAPNHDRYAAISD
                     AVMATFASVTDRYEPLSLDEAFLDIAGAVRRLGPPTSIAQWLRDTIADEQSITCSVGV
                     ASTKFVAKLASGLAKPDGLLVVPQDEVLAFLHQLPVGALWGVGDRTEEALQRLGLHTV
                     ADLAHTPVDTLRRALGDATGTHLHALAWGRDERRVLPQQREKSVGSDETFAQDIDDPV
                     LIHRELLRLADRTAARVRSAGLMGRTVTLKVRFADFTTITRSRTLRDPTDVSREIYAA
                     ATALFDALGLQRARIRLVGVRMEGLVESERAAVQPRLDDPDFGWRDADRAVDRASARF
                     GAGAVRPAALVRGESVAHPGSMPRGGE"
     gene            complement(36213..36998)
                     /locus_tag="PLB86_00745"
     CDS             complement(36213..36998)
                     /locus_tag="PLB86_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRC63693.1"
                     /translation="MSEEQQRRARRAATGVRTAALWAAVKDLVTERAAALGRPLRVLD
                     LGGGTGELAVPLAELGPDLVADVTVVDPSPDALAALGRRAAESGVQARVHGSQGDADT
                     LADLLPERAFDLVCCHGVLEVVDDPTATLRVLADALADDGHLSLLVAGRLATVWARAL
                     AGEFGQAHTALTSPDGRWGAHDPLPRRFDLAQLRALVESAGLQVEHWHGVRLLADLVP
                     STSIDTDAERAALVALEEALAGHPAYPFLGELGAALHLLARRG"
     gene            complement(37008..41477)
                     /locus_tag="PLB86_00750"
     CDS             complement(37008..41477)
                     /locus_tag="PLB86_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRC63694.1"
                     /translation="MSTFVHLHVASGYSMQYGASTPAALVARAAAFGQPALALTDRDG
                     LYGAVRFVQACTEAKIAPILGVDLAVHLPPSDPATGRYAARPGSFSSASSVSSTGSAS
                     ASTSSTAPPVWARPSAPVRTPVRGGAIVDPRLPRVTVLARGLAGGVAPGLGWARLCRL
                     VTRTHLSGERGNPLTTPQVLAELAAPVGDDPAALIVLLGPDSDVGRAVLAKRRAAAYQ
                     ALERWRAVLPAGALALETVCHGGPEGTPASLGHAARMLALGRDLGVPVILSAQVRHAD
                     PAEAATVDVLDAARRLVALDVRHLDRVTTAGHLAPTPAMYAVACDIVQASGPGLAGGT
                     STVRDGARDLLAATVALASECVLDPAIDLGIGGVHLPEQSTLGLGLHDDPQHHLVQRC
                     RGAVGARYPGATDSEVARISARLDEELEVIAALGYPTYFLTVATVTDLIRDMGVRVAA
                     RGSGAGSLVNYLLGISGVDPIRYDLLMERFCSPLRAQLPDIDIDVESARRTEIYEKIL
                     ARFGGDRVTCVSMMDSYRVRHAIRDVGAALGLPPGEVDAMAKAFPHISARHARSAIAD
                     LPELRVSGLGAARYDLMWDLVERLDGLPRHIALHPCGVILSNAALLDRTPVEASWIGF
                     PMSQFDKDDVEVMGLLKLDVLGIRMQSAMALAVDEVARVDGIPVDLDDRAQVPLDDEA
                     TFAMIRTTRTLGCFQIESPGQRELIGKFGPERFEDLVIDISLFRPGPVKSDMIVPFLK
                     ARHGWAEPEYLHPTLIPALAETAGVVVFHEQVLRIVAETTGVSLAQADEVRRALGSPQ
                     GQLDVEAWWRPAALARGYTVADADRIWEVLRAFASFGFCKAHAAAFALPTYQSAWLKT
                     HHPAAFLAGVLTHDPGMYPKRLILDDARSLGIEILGLDVNASKSWYAVERLPHPLPGR
                     PPHPLPSRGISPSSADGGIPRPGREPGKEWDPDLPDGRGYGIRVSLTDVKGITEAEVT
                     RIVAGQPFTDLADLAHRAQVSRPIMERLVLAGGLDSVYGISTATGAGADRSFGAGGLG
                     VRERVTRRDLLLHVAELDRWVRSGGSGQGKALRGGRSRFTRRPATMSATGPGGDAAGA
                     PVTDTRALAAAHPRREVAVLVQQPGVPAGPGTIRAFDVAALAAAQSQAAAPVHVSDRH
                     TQMVLPLGDTPTLTRGSGLPELSGPERVRAELEILGLDASAHVVAHYEPMLEALGVTR
                     SRDLLSARNRSEVWVAGVKVATQTPPIRSGRRVVFLTLDDGTGPSDSTFFEDVQGPYA
                     ATVFHSWLLLVRGVVRRTGARGISLRATGAWELSGLWEAFATGGRPRLLDALAAADAA
                     AHDRAAAAAAAYALPYAVPRGTGWAEPAADAAAAAADGSPVGDRDDTAYADRERAARL
                     DHAASLVGGRPASRAGGMGGGSPTSPVGAPGAPRQVLVHASGFKQSPYADTRPPGEDV
                     KSARHLAGAVDHAGLTDQVTPDAFDAIDAAGQGSGAGRPPRKLWHSSPGSSGH"
     gene            complement(41516..42013)
                     /locus_tag="PLB86_00755"
     CDS             complement(41516..42013)
                     /locus_tag="PLB86_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV_6107 family HEPN domain-containing protein"
                     /protein_id="HRC63695.1"
                     /translation="MSAAPSHPYPATPSPPLRPRRHVPPSATVMELLDRAQASLLYAS
                     QADQVGERYVLAHLAGLRAAAAVLAARTTPARSSRPRSVWEVLPTLAPELTEWAVFFA
                     ASARQRAAVERGDRVLPAREADDLVRQAEAFIEVVQDALGVPRTFVRPDYLAPVTPLA
                     RHAGR"
     gene            complement(42081..42545)
                     /locus_tag="PLB86_00760"
     CDS             complement(42081..42545)
                     /locus_tag="PLB86_00760"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HRC63696.1"
                     /translation="MVRRYAEPVRVGTASRLTPDSLADLEQVPPGPGGYAASSPTWFE
                     WAGRRYHVRAILAHWRERRAWWREVLDADPTLEATSSGPASNTASTPASNTSSDTDCR
                     SAIAVAARERLVWRVEAGPGWLTRTGVFDLACDETSPGDPDPDGWLLLRVSD"
     assembly_gap    42792..42891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <42892..43031
                     /locus_tag="PLB86_00765"
     CDS             <42892..43031
                     /locus_tag="PLB86_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRC63697.1"
                     /translation="LTTLVDSALAAHDVVWAAAGHPHAVFPTTFAELIRISAGRAATV
                     A"
     gene            43285..43965
                     /locus_tag="PLB86_00770"
     CDS             43285..43965
                     /locus_tag="PLB86_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HRC63698.1"
                     /translation="MGAEILPWVFTSGWASGINAYAVVLIMGLAEKFWQVQGIPDALG
                     RTDVLIGAGVLFVIEMVADKIPYLDSTWDSIHTVVRPAVGATLGYLLGNESADLNAAF
                     TAATGGILALLSHLVKAGTRAAVNTSPEPASNVVLSTAEDVTVAAVVTTAFANPWLAA
                     SIAGLLLVVGMVTVLFLLKRIRRFRARYNAWGDRLGSSEPKADGSLRDAPARRGDTAR
                     LPQGPGAD"
     gene            complement(43995..44906)
                     /gene="metF"
                     /locus_tag="PLB86_00775"
     CDS             complement(43995..44906)
                     /gene="metF"
                     /locus_tag="PLB86_00775"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRC63699.1"
                     /translation="MAVLPRAAHLVRSIPDALAQGRPSISFEFFPPKDDAGEAALWEA
                     IRDLEKTRPTFVSVTYGAGGSGRDRTIRMTDRIAQETTLTPMAHLTCVGHSVAELRSI
                     IGHYVNVGVRNVLALRGDPQGGLDQTWERHPDGLDHADELVSLLRTLGPFTVGVAAFP
                     DRHPESPSLDHDADVLVRKADAGADFAITQFFFTREAYVDLRDRVVARGRDLPIIPGL
                     MPVTNVRQLARMTQLSGQPVPREVTERLERVADDPVAVRAVGVEIATELAQGLLDEGA
                     PGIHFITMNRSTATLQVWGNLGLEAAQ"
     assembly_gap    44941..45040
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<45041..46047)
                     /locus_tag="PLB86_00780"
     CDS             complement(<45041..46047)
                     /locus_tag="PLB86_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63700.1"
                     /translation="MSHPSNAPSNSPTDADLEDLLRVGLREAVDRADAAYLPLEVSEI
                     VAEGARTLSRRRRWQVLGAAAAAAVAVVGSWALTGGSGTRLSQQPATVPVSTVAPTTS
                     EEIFVQDERFGVRVESGEVSTALNVIGSVAPAVLQWGPPPGSAGAHEVLVLIPEADHP
                     DPGSVDVALVPDVRRLGEARQIIVNGRLVIVVRHTPPPTGMTPYAGLTYTIGGREVRH
                     LSPGILHIDGVGAGAARNEGGDFGYAVRLEAGTVAVSLQRRVGDLDLGGGVFTLPAGS
                     PGLLRLGDGADFDMAYALVRGTPPRAVTVTGDTPSRFAGGVSWGGMTIDDTDVTLVLI
                     AL"
     gene            complement(46044..46553)
                     /locus_tag="PLB86_00785"
     CDS             complement(46044..46553)
                     /locus_tag="PLB86_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HRC63701.1"
                     /translation="MTPQDRERDASFAQFVEVASPGLLRTAWLLTGSSEAAHELVQAT
                     LVKAYVRWPQIRSESAMAYCRRILANERTDRWRRTRREVPVAEVDTRSADRSDTATSD
                     DRDEVVRLLRGLPEQQRRVVVLRYYCDLSEDTVADTLGISVGTVKSSASRALAALRAQ
                     LVVQEGEIR"
     gene            46704..47792
                     /locus_tag="PLB86_00790"
     CDS             46704..47792
                     /locus_tag="PLB86_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRC63702.1"
                     /translation="MTNPLDRESLRSRVQSALEAELDHQEQVLAELDEDLTPLVAPVR
                     SLLAGGKRLRAAFAYWGYRAAGGADSDSLVRFASSMELFQAAALLHDDVMDGSDTRRG
                     MPSAHRAVAAIHAQHAWSGNADRFGEATAILAGDLCLQWTDEVYSTSGLPAEHLARGR
                     ATFDRMRTQLMGGQFLDMLESVRTWEGRSTDERIAAARKVIRFKSAKYTIEQPLLIGA
                     LAGGVDPAAVPGLSGYGLALGEAFQLRDDLLGVFGDPEQTGKPAGDDVREGKRTVLIA
                     YTLDVADAGQRAELTRSFGRADVDVAGIESVRGIIRATGAVERVESDITRLTHAATHA
                     LGEPAAYLQPEASTVLGELVALATDRVT"
     gene            complement(47907..48314)
                     /locus_tag="PLB86_00795"
     CDS             complement(47907..48314)
                     /locus_tag="PLB86_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HRC63703.1"
                     /translation="MAEPQLASEADDDSDDPRALETLVGDWSYLPDIAERYGVALSRV
                     RRFLADRELLTHRVGPNRALAVPTDFLGPDGPRPELKGTFTVLADGGMSDVEIIRWLH
                     SVDTSLPGGSTPLAALSAGFKTEVRRRAQALAF"
     gene            48449..50455
                     /gene="pknB"
                     /locus_tag="PLB86_00800"
     CDS             48449..50455
                     /gene="pknB"
                     /locus_tag="PLB86_00800"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HRC63704.1"
                     /translation="MTSGPGLHADERVLGGRYRLLSRIADGGMATVYLAIDERLGRRV
                     AVKVLRADLARDEGFVGRFQREARLAAGLSHPNIVAVHDFHESSGVPGEELYLVMEYV
                     DGRTLREKLVDGGAMSVRQATHIITAVLAALDEAHRADLVHRDVKPENVLVRDDGTIK
                     VTDFGLARAVTSSTRTSTTGILMGTVSYLAPEQVDGDRADARSDVYAAGLMLYELLTG
                     QRAFQGENPAHVMYQHVHGEVPLASDRVSTVPLELDQVIARATARSPDDRPDTAGDLI
                     QVLRDAIRQLSDDEFDAAPVFLAGMAPGTQPVARTASPTKSLDADLVGAEGDPSPTER
                     FAAVELGATQRLRTIELDEDGDGTGEVSAPAGEPVKQKRRRRIWPWLLTLLLLVGGGV
                     GGWWWFDGTGPGSLRIVPTLVHLSLADAEKALASVDLRSATTEAFSETEPKGQVISAD
                     PKPGAEVSKRSSVALVVSKGQERYAVPTLTAVARGDVERLLKERSLALGTVTEDFSET
                     VAKDVVISQDPPPDTLAKRDTPVNVVISKGRAPIPVPNVVNKTADEAQKALEALGLKV
                     VRGDPVNSETVASGAVVSQTPATGTLFKGDTVTIIPSKGPVLVQVPDVTLKKVNDATK
                     ILKDLGLEVKVSQIFGGVLKTVRFQDPAAGTMVRRGTVITLTVI"
     gene            50472..51380
                     /locus_tag="PLB86_00805"
     CDS             50472..51380
                     /locus_tag="PLB86_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC63705.1"
                     /translation="MAMSPARARTVATILLVTLTAVWGSTFFLIRDLVQTVPPADFLA
                     VRFTLAAVLMVVIFWRQLRGLSRRDWLVSAALGSLYGFAQILQTIGLAHTDASVSGFV
                     TGTYVVLTPVFTALLLRERVPASTWYAVLLATAGLALLSLNGFSVGLGEGLTLGAAAL
                     YALHIVGLGRLSTPDRATGMSAVQMIAIAVICVVAAVPDGMVWPSGVGQWGSVVYMAT
                     AAGVLALWAQTWGQAHMSATRAAIVMTLEPVFAAGFAVAFGGEVLTWRMLVGGALVLT
                     AMYTVELIGRRPDAPNRPAEALHHEV"
BASE COUNT         7816 a        17878 c        17643 g         7649 t
ORIGIN      
        1 acgccggacg agatcgacac cgcgaccgtg aggacgtagt cgaccagcag ggccgaggcg
       61 acggtcagcc cggccttggg gccgaggttc tccgtcgcca cctcgtagtc gccgccaccg
      121 gacgggtagg catggacgtt ctggcggtag gacgccacga cgatgaccat gacgaagatg
      181 acaccgagcg tgatctgcca cgagtgcatg gcggcgaagg caccgccagc caaccccaac
      241 atgatgagga tctcgtcggg ggcataggcg accgacgaca gggcgtcact ggcgaagatc
      301 ggcagggcga gcttcttggg caggagggtc tccccgagcc gatcgctgcg catggcacga
      361 ccgacgagga tccgcttggc tacttggccg agagcaggca caaatcgcat cgtatgccgt
      421 tgcgcggcat acctgcgcac cgtcgtcgcc ggtgtgagat gcgcgttacc gccggtgtag
      481 cgtcggagcg tgcacttcgt catcatgggc tgcgggcgcg taggtgcgac cctcgcgcgg
      541 agtctgtccg acctcggaca cgacgtcgcc gtcatcgaca tggaccccac ctcgttccgg
      601 cggctgggca gttcgttcga ggggcggacc atcaccggca tgggcttcga ccgcggagtt
      661 ctcgtggcgg ccgggattcg agaggcatat gccttcgcgg cggtctccag cggtgacaac
      721 tccaacatcc tggccgcccg ggtcgcccgc gagaccttcg gggtcgagca cgtcgccgcg
      781 cgcatctatg acccgcgccg cgccgcggtc tatcagcgcc tcggcatccc gaccgtcgcg
      841 acggtgcgct ggacggccga tcagatcatc caccgcctgc tgccgaccgg ctcggtgcct
      901 gcgttcaccg accccagcgg ccaggtggtc ctcgccgagg tccccgtggc cgcggcctgg
      961 gtcggtcgtc gcgtcacgac gatcgaacgg ctggcctcga cgcgggtcgc cttcctcacc
     1021 cggctcggcg aaggcatggt gccggacgcc gacacggtct tgcaggacgg cgatctcatg
     1081 cacgtctgcg cgcccgcgac cgagctcgac cggttggagc gcgtcttcgc cgcgcgcccg
     1141 ccgcacgaat aaccaccccg ccatctccag cccagccaag gacatcccat gcgcgtcgcc
     1201 atcgtcggag ccggcagcgt cgggcgatcc atcgctcgcg agctcctgca caacggccat
     1261 caggtgttgc tcatcgaccg tgacgccgac gatgtcgtgg cctcccgcgt ccccgacgcc
     1321 acctggctgc tcgccgacgc ctgtgagatc tctgccctcg aggaagccca cctggaggcc
     1381 tgtgacgtcg tcgtcgccgc aaccggcgac gacaaggcca acctcgtcgt ctcgctgctg
     1441 gccaagacgg agttcggcgt cccccggacc gtcgcccgcg tcaacaaccc ccggaacgag
     1501 tggatgttcg atgaggcctg gggcgtggac gtcgccgtat ccaccccacg tctgatgacc
     1561 gctctcgtcg aggaggccgt cagcatcggt gacctcgtgc gggtgttcga gttccgccag
     1621 gccaagtcgg ccatggtcga gatgaccttg ccgccgacca gcccctacgc cggcgcgcgg
     1681 gtgggcgaca tcccctggcc cgaggacacc gttctcgtgg gcatcatccg ggagggtcgg
     1741 cccatcgcgc ccagccgcga cgatgcgctc gagccgcaag atgaactgct gttcatcgcg
     1801 gcgcgcacca tggaagacaa cctcgagaac ctgctctccc ccggccacct gccgcgcggc
     1861 aaggannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntagag cgggatctgg
     1981 atcgagaggc gcagcacggc cagccccacg agcacccagc cgagccggct ggccaccttg
     2041 acgatcccgt cgtgacggcg ccaggacgtg aacgcgtggc tcatggcctg cgcgtcggcc
     2101 tcggcggcct gctccgccgc ggcccgctgc tcatcggcca tccgggccat cgcggcccgt
     2161 ccctcgcggg aaccggcgaa ccggacccgc gcgctggttt gcatgagctc cgggtccccc
     2221 gccgcgatga ggaagccgat gaccggccag cgcacgaggt tggagatggt gaagaccagc
     2281 agggagaacc cggtctggag catccccggc aagaaggcat cctgggcccg cccggtgcgc
     2341 gtggccacca ctcccgaaat gacgacagcg atcaccgccc acccgacata gcgcaacgtc
     2401 tgaccctggg ccaggcggat cccggcgatg acgacggccg ctgcgagggc cgacagggca
     2461 gcgaggggga tgtcgctggt gacgttccat acgacgacga agaggacggt cggcacggcc
     2521 gactcgacgg cgccgcgcca gcctccgaac acggtcccga ggcgggcccg caacacctct
     2581 tcgacggtga cgaacgactc ctgggcggcc ggacgacccg catcggtctg atcagccatg
     2641 ctgcggcagg atctcgtagg cggggttgta gatcgtcggc ccggcgcgca ggtcagtgac
     2701 ccttccgtgg atccgcagca tgaccccggg gacgatcccc gccactgccc gacgaccgag
     2761 ccagacaagg atgagcgtgc ccgttccgtc gaagacctct gcgaccaggg ccgggacctg
     2821 cacctgaggc cgcagcgcca ctgagcgaac ctcgccgctc acggtcaccc gctgccgagg
     2881 gaccaacgct gagatggggg tcgctcccag gcgaaggctg gcgtcgtgga gctcgtcagc
     2941 ctgcaactcg gccgacgact tggtcagccg acccatgagc cggctccatg tgctcgactc
     3001 ggaacgaccc gggctgccct cgtcagacat ggctagtgga cctcggtgat ctcagggccc
     3061 cgttcgaagg gctgcaggtc atcgaacccc gccggggtcg gctctccccc aggcgatgcg
     3121 tcgggacgcg cggcttcggg gccgtcggcc tggggcagcc gaaggggcag ttgctcgcgc
     3181 ggcgccatcg cctgggtgcc ccggtcgacc accgtcttgc ggatgacctc gatgagcgcg
     3241 tcgccggcct gctcgtcgac ggcggcgggg ccggtgatga gcgcccgaag gaaccaacgg
     3301 gggccgtcca ccccgagcag ccggatcggg acgccgccgc gctggtgcag ccgcaactcc
     3361 ttgccgagcg ggccgctcac gagctccgcg cgaccgcctg ccgcgacaac cgattgagcc
     3421 acctcggtgc ggatctcagg ccagatgccc tcggtgcggg gcgcggcata cgcctgcagg
     3481 taggcgcctg acgtgcccaa ggagagttgg atgccgaccg gggtctgcgt cgactcgtcc
     3541 acctgcaacg cgacctggac gccgtccgtg atcgccaccc acaggctgcc cagctcgacc
     3601 cgagtgtcgt cgcgggtcac ctctgcccgg tcgaaggggc cgcgcagccg gctgaacggc
     3661 gtctccggct cccagtcgtc ggactcggcg accgtcgcgg cagtcgcctc agcgtcactc
     3721 ggcagggacg ccggtatgcc gtccgctccg gtctcggcca ccggccgttc cgtctcgtcc
     3781 ttggcgcgct tgcgcccgaa aagtcccacg ttgcgtcatc cagtcctgtt gggcagaggt
     3841 cgttcggcct gcggcggcgg ggtcaggccc cgaagccgcc actggctccg tgtccagtgt
     3901 ccccccgggg gctgtccggg agcgaatcga cctccaccca ctgcaccgct tcgacgcgtt
     3961 ggacgaccaa ctgggcgatt cgatcgccgc ggctgacctg aaagctctcg gaggcatcaa
     4021 ggttgaccag gttgacgaag atctcaccgc gatagcccga gtcgatggtt ccgggcgcgt
     4081 tgaggatggt gatgccgtga cggatcgcca gccccgagcg ggggtggccg aacgcggcat
     4141 acccctcggg gagggcgatc gcgatgccgg tgggcacgag cgctcgctgc ccgggggcaa
     4201 ggagcacgtc gacacgcgcg aacaggtcga tgccagcgtc gcccggatgg gcgtaggccg
     4261 gcaggggcag gtccgggtcg agtcggtgca cctggaccgg cacgccgggc atcaggcgac
     4321 gcactcccgg cagaccatct gcccaccgac gttcttggcc aactgggacc ggtggtgcac
     4381 gaggaagcag cgggaacacg tgaattcgtc ggcctggcgc ggaatcaccc ggtaggacaa
     4441 ctcctcaccg gacaggtcgg cgccgggaag ttcgaagcct tcggcctgct cggtctcgtc
     4501 aacgtcaacg gtcgaggacg ccttgtccac acgtcgggct ttgagctcct caagggagtc
     4561 ttctgacaga tcgtcgtcgg tcttgcgtgg tgcgtcgtag tcggtcgcca tgcccgttcg
     4621 gtctcctcca tcactgtcgg cctgcggtgg ctcgtcgcgg ctaacgctca gatcgtcgaa
     4681 tttgtgcccg gctgcaccgg ggccaacgac acgtcactgc ctgcggggcg tgattgtgcc
     4741 ctattcagag gccgagcgca catgcgtttc gcatatcgat tgggtcacac aggacgggtc
     4801 gcccctgcaa gcggtcttac ctccgatcgc tccgagggtc gacctgcgcc ctaagccggt
     4861 gactccagca gcgccagcag ccgagggtgc agggccgggt tggccgcgac gaccaggtcc
     4921 acaccaggcg cgccaccgcc gaccccggag acagatcctc cggcctcggt gacgaccaac
     4981 cacccggctg cgaggtccca cgggttgaga ccgacctcga agaagccgtc cagtcggcca
     5041 tcgccgacga ggcacaggtc gatggcggca ctgcccatcc ggcgcaggtc gcgcacggcc
     5101 ggcaggacgc gggtgagcac ctcggcctgt tgggctcggc gttcgggctg gtagccgaag
     5161 ccggtgccga cgagcgcggg gccgaggtgg ctctggcggc ccacccgcgc cgggaccgcc
     5221 ggcggctcgc ccccgggccc ggcgacatcc cacggccgcg caggtccgcg ccgccacgct
     5281 cccccgccgg cccgggccga ccaggtgatg tccagggcag gggctcggac tgctccagcg
     5341 accggacgcc aggcgcccgg cgtggtgggg tcgcccacga cgacggcgac ggacacggca
     5401 tacaggggaa ggtcgtagag gtagttggtg gtgccgtcga tgggatcgac cacccaggtg
     5461 agcccgctgc gggacgggat cgagctgccc tcctccccct gcagaccgtc gtcgggacgt
     5521 gccgccctca accggcgccg gaggtgctcc tcggcacggg tgtccatgac ggtcaccgga
     5581 tcggtgtccg acgtcttggt ccctgtcgtg ttggccaggt cggccggtcg cccctcgtgg
     5641 accagttgac cggtttcgtg agccagggtg agcgccaatg cctccaaaac ggacaggtcg
     5701 tcaatggatg ggacgggcag gtgcccggtc gtgtctggga tcggcgtcat ggtgaggtcc
     5761 acggtaacgg tccggcgtgg ccgcttcgca gacctcggtt cgaatgagag agtcgtgacg
     5821 acacgtcccc agccgaccca tggagtgagc atgcaggacc tgcggttgat cggcgttgac
     5881 gacgacggcg ggcacctctt gctcgccagc gtcgacggcg cccgattccg ggtcccgctc
     5941 gacgagtcgt tgcgggctgc ggcccgccgt gaccgtcctc ggctgggaca actgcagatc
     6001 gagatcgagg gcggcatgcg accacgcgac gtgcaggcga tggtccgggc ggggatgtcc
     6061 gccgatgagg tcgccgagcg ctccgggtgg acggtcgaga aggtccacaa gtacgaaggc
     6121 ccgatcctcg ccgagcggga gtatgtcgcc ggcctcggcc gggcggtgcg gttgcggcaa
     6181 cgcggcggat ccggcccctc gaccacgctg ggccaacgcg tcgccgatcg actcaccgcc
     6241 cgcggcgtcg aggccgactc gatcgagtgg gacgcctggc gccgcgaagg acccgagtgg
     6301 acggtcgcgg tcaacttcag cgccggtggg cgggtgcgcc agggcacctg ggccttcgat
     6361 gtcgccagcc gttcgctgca tgccaaggac gatgaggccc gctggctcag cgaggacgaa
     6421 cctgcggcaa ccccggtccc gcgtgaagca ccggtctacg acgtcgaagc cgacggcgga
     6481 ctggatgcgg ccggacgccg ctctcgggcc gacgacccgg tcgacctcgt cacggcgatg
     6541 cgtgagcgtt cggcggcccg gtctcgccgc gggtcccgcc gtcgcgggtt ggagccggtg
     6601 gctgccctgc ccttgggcga tgccgtgcag tcgccccccg acgaccacgt cgacacnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgc cctgcccttg ggcgatgccg
     6781 tgcagtcgcc ccccgacgac cacgtcgaca ccgttgaaac catcccgagc gacgtcgccg
     6841 ccgccgaaga gcgccggccc gtcgcggtga ccgccgccgt cactcgcgag gccgagccgg
     6901 agcttgccat cgcgcccggt cccgactcaa ttaccgaatc cagcaccgcc gccagtaccg
     6961 actccaacac cgagccggac tccgagccca agcacgaacc ggccttcgaa cctcgccagg
     7021 accccaagcc tgaggtagag gtcgaggaag tttccccgga accggcggtg gcacggcata
     7081 cccctcggcg ctccggtcga ccctcggtgc cgagttggga cgacatcgtc ttcgggacca
     7141 agccgaagga ccccaacgcg taacgccgtc ggcgcagagc gcgggtcagc cgtcctggcg
     7201 cgcgacgtcg aagggcagaa cgtccggtga gagcgcggcg gcgcgggccg cgtgcgaggt
     7261 catccgacgc atgtagtggc gccggcagag cacttcgtac tccacgaccc cactgccctt
     7321 ggccgtgtcg ccgaccacca cctggtcacc ttcgatgacc atctccccgt cgatgacgcg
     7381 ggcattgtgg gtcgcgcgga cgccgcacca gcacaacgcc tcgacctgca gcacctggac
     7441 tcggtcggca agctcaacca agcggcgcga cccggggaag agctccgtcc ggaagtccgc
     7501 catgatgccg aaggcgaaga cgtccacgcc catctcgtcg acgatcctcg ccagttgctc
     7561 gatctgccgg ggcgtgtaga actgcgcctc gtcgcacacg acgaagtcca cccggtgacc
     7621 ctcggtcacg aagccgacga cggtgtccca gaagtccagg gtgtcggtga cctcgatggc
     7681 ccgccgggac aaccccaggc gcgaggacac gaccgcctca cccgcacggt cgtgcttggt
     7741 gaagatcaac ccgcccttac cccgcgcccg gtggttgtgc tccatctgca gggcgagggt
     7801 cgacttgccg gaatccatcg tcccggaaaa gaacaccaac tcggccacga cgcccgaccc
     7861 tatgccgtgc gggcgggcca ctggaacagc gggatcgcga tttcgtcctc gctgaccgag
     7921 ccgtgcagcc ccaacagggc gatgagctcc ggtcgcatcc gaaccgaatc gacgacggcc
     7981 atgctgccca ggcagttgac caagacatcg ccgatgcgtg gcaacacccg atcggccacg
     8041 gaccccgctg ggccgaagag cccagcctcg atggcctcgg accggctgat caccgtggcg
     8101 ttctccccga ggcgctcccg ccatgtctca gacacgtcct gggcggcgcc tggcgcgcaa
     8161 tagagctgca ccgaacgggg ttcgcccccg acgtgccgga ccccgtcaag cagccgcggc
     8221 tcgaccgcga gatcgatccg gtccgcgaac ggaacctcga ccatcccgtg gtcggcggtg
     8281 acatagacgg ccgtatcgga ggggacggag gccaccagcc ggcgcaactc ggcgtcaacc
     8341 cgctcgacct ccgcgcccca ctccggcgag ctcgggccat ggacgtggcc gagcttgtcg
     8401 acgtcgcccc agtagaggta aaccaaagcg cgcggggtcg ctcggaccgc ggcgagggct
     8461 tcgtcgacgc gggtcgcgag ttggtatgcc gcgcggaacc ggcccccgcg tagcgccgcg
     8521 ttggtcagcc ccgaaccgtc gaagaagccg ggaccgaccc gcgtgacggc cactccttcg
     8581 gcctcagcgg cctcgaagac cgtcggctcg ggctgccagg tgaacgggtc gggcccgtcg
     8641 tcccaactga gctcgttgag gatgcggtcg gtctcgggcg tcgcgcccgg gacgagcact
     8701 tcgtaaccga ccaggccgtg cgcccccgga ggcagcccgg tcccgaaact gcccatcgac
     8761 gtcgccgtcg tcgatgggaa cccacacgtc aatcggtatg ccgcggcgag cccggaccgc
     8821 aggaacggcg cgtgaccggc ccggcggcgg acgagcgtgt cacccaagcc gtcgaccagg
     8881 acgacgacag cgcgacgggc gggggtcagc gggatcaggg ggcgggcggc ataccggggc
     8941 gacccgaggg aggccaagac cgacggaagg accgccgaca gggtcccggc cccgaccggg
     9001 agggccggcg gcgcagaacc cacctcagcg gccgatcgtc gccgacagcg ccgaagcgaa
     9061 gtgaagcgcg cgggccaggg ccgcgtcgcc atcggcgtcg gcgctgatgc gcagtgagat
     9121 gtcgtcggcc gcgagtgtcc cctcgaagcc atggtcggcc tcacactgcg ggtcaccgca
     9181 cgaagcaggc atgacgtcca ggcggctcac ggccccccag cccagtgtca gggtgatctc
     9241 ccgaccgagc gaacccggca cgtaggcatc gggattggcc acaacgtggg cgagcatgac
     9301 cccgcgcacc gcagccaacg ggatgctctc actcgtcgcc gtcgcgacgg cgcgctcgtg
     9361 gtggtcgtcg tgatcgtcgg catggacgac gacgagtttg gtcgccgtga ggacgagaac
     9421 ggtgatgtgc cggcggacgg agtcctggtc gaaggtcgtc tcctggtgga ccaagtgggc
     9481 ctggacgaca tcaccggcaa gggtggcgcc gaccacgtcg gccacgaggg ccgggtagta
     9541 accggcgtgc tggatgtccg acctcagctc gacgggcagg ctgaaaccgg gcacctcacg
     9601 gggaacctta cgcatgatgg ccattctctc ccatggccgc ctcggtcagg tcatcgcgcg
     9661 gcgactggcg tccttgcggg tcatggccgg ggccacgaag atctcggccc cgaggacgtc
     9721 gacccctgcg ccccaacagt tcaccggttg cagcgacacc gacgccagcg tcgagtggtt
     9781 atcggcgagc gtcgacactc gggcgatgag atcacgcagg tcatcgaggt agatcggttc
     9841 cttgccgcca tggccagtga gcagcggggc agccttgacc gaaaagatca ggtcgtcgac
     9901 atcgacgttg gtcaacggcg ggatgcggta gccgatgtcg ccgagcagat cagtgggcgg
     9961 gccggcgatc gagaacgaca cgacggggcc gaagagcggg tcctcggtgc tgccgacgac
    10021 gcaggacact cccggaggtg acatccgctg gacggcgaac cggtcggcca cgaaggcgcc
    10081 cagccggttg gtcagcgacg catgggcatc gcgcactgcc tcgggtgagg agaggtcggg
    10141 acggactccg gtcacgccca cctggtggcg cagcgccggc gcaatcacct tgaggatgac
    10201 gggatatccg agccgctcgg ccgcctcgac cgcctcgtcg gcgttgtgaa ccaagtcggc
    10261 gggccacaag ccgatgccgt atgccgcgag cacctggatg cactcgtcct cggtcagtgc
    10321 cctcccgtcg gggctgtcgg tgaggacatt gtcgatgagc tcctcgacga cacccttctc
    10381 gatccccgag ggggccacga gcgtgccctt gtcgcgagcc cgccactgcg cgtagcgagt
    10441 tgccgccgcc accgctcgca cggcgtcttc aggcatgggg tatgccggga ccacccgagg
    10501 gcgctcgccg ggggtgttca aggtcagttg ctctgtgacg ccgcgcatgc cgaggaacgt
    10561 ggccgtgcag gtcttggtgc tgcgtccggc ctcatcccgg accgccgcgg cgaccaactc
    10621 gtcgtcggtg tagatggggg ggatgaacga ggcgatcacc gagtcgaccc cggggtcatc
    10681 gaaggccgcc cgcaacgcga gcgcgaactc ctcggcgccc gcctcggggg caacggagac
    10741 cggcccgtgg gtgaccttga ggtcccaact ctgggcagcc tgcgtcgtca gcgaacccag
    10801 tgcgtcggaa ttggtgacca cagcgacgcg atcaccggcc ggaagctcct ggtgaacgac
    10861 gagttgggcg acgtcgaaca gttgatggat gttctcggtg cggatgacgc ccgactggcg
    10921 caacagcgcg tcgaacgcct ccgtgccgac actcatcttg cgggtgaggt gacccggtgc
    10981 cgacccgagt cgactgcctg cggacttgac gacgatgacc ggttttgcgg ccgccagcga
    11041 gcgggcgatg cggacgaatt tgcgcggatt gccgatggat tcgacgtaga gacccacagc
    11101 atcggtggac gcgtcctcga tccagtactg cataaggtcg ttgcccgaaa ggtccacccg
    11161 gttgcctgca gaggcgaaca ccgagacgcc caacccccga cgggcggctg aggcgagaac
    11221 cgcgatgccg agggcaccgc tctgggcgaa gagcccgaag gagccgcgcg gcggtagttg
    11281 cggcgcgagc gacgcgttga gccgcacgtc ggggtggttg ttgatgatgc cgaaggagtt
    11341 gggcccgatg acccgcagcc cggcccgacg ggccttcttg cgcaacttgg tctggagttt
    11401 ccacccctgt tcgccgtcct cagcgaagtg agccgacggc acgacgatcg ctcgggcgcc
    11461 cttcgccgcg cactcgtcga cgaggttgat gacgtcgggc gcggccacag cgacgacgac
    11521 gaggtcgacc aggccgggga cgtcggccag cgtgcggtag gacttgagcc ccatcacgcg
    11581 cttggcgttg gggttgacgg catacacctg cccggtgaaa cccccctcga cgaggttctt
    11641 gaggaaggtg tgtccgatgg agaactcgcg cctgctcgca ccgacgacgg cgaccgaacg
    11701 cggcgtgagg atgcccccga cgctgatcgc ctccgaacgg tgctcgcggg cataacgcac
    11761 ggccgtcgac gtggccgtcg ggctgatgtc gaacgacagc gagatgaccc cgtcctcgag
    11821 ccggtgcttg acgttgtagc cggcctcctt gaagacgttg atcatccgac ggttctgcgg
    11881 gagcacttcg gcgacgaagc gggtgacccc gccgtcgagg ccgaccagcg ccaggtgttc
    11941 caataggatc gagccaattc ccttgccctg gaaggcatcc gacacgttga aggcgacctc
    12001 ggccctggtg tcgtcgatct tgtcgtaacg cccgatcccg atgatcaggt cccgatgcat
    12061 cacgacgagg gcgacccgtt cgtcgtagtc gacgacggtg aaccgttgga cgtcacggtc
    12121 ggacaactcg cgcaacggtg cgaagaagcg caggtaaatc gactcctcgc tctgcccggc
    12181 atgaaagacc cggatgcgct cgtcgtcgga cggcctgatc ggccgcacat gcgccaccga
    12241 gccgtcactg aggacgacat ccgcctccca ctcgaacgga tagccgggtg ggtgttcgtc
    12301 gccgccaccg acgggttcgg gcgaggtcgg ctcgtccggc aacgggtgcc ggtgttggcc
    12361 gtgcttgctg gacacgccca catgttccca cgccaagggg tcacgggttc cctgcggggt
    12421 ggcagcatgc gaggtcatgg agctttctcg cgcgatccgt ggccgccgga tgatccgtcg
    12481 ctacgaccct gaccggcccg tgccggacga ggccgtgctc gcctgcctgg gggccgccat
    12541 ccgagctccc agtgccggtt tcagccaggg ctgggatttc ctcgtgctgc gctcccccga
    12601 ggaacgggcg accttctggt cggcgacgac gggcgatcaa ccgccggaca catggctggc
    12661 cgggatggcg accgcgcccg ccctcatcct gtgtctgtcc gaccccgagc gatacctcga
    12721 gcgttacgcg cagcccgaca agggctggac cgaccgcgac ccggcgcgct ggccggtccc
    12781 ctattgggac gtcgacaccg gcatggcggc gatgctcgcc ctgctcaccg cggtcgatgc
    12841 agggctggga gcctgcttct tcggggtgcc gccgacgcgg cataccgagg tccgttctgc
    12901 cctggggatc cccgatgacc gccgcttcgt cggggtcatc agcctcgggt atgccgtgcc
    12961 ccacccggcg agccccagcc tggcccgcgg gcgccgcggc ctggacgagg tcgcgcactg
    13021 gggccggttc ggggtccacg acccactccc ctgactccgg cgcgcctgac cccctcccgc
    13081 cctgcacgga gatagcgctc gcatggccac ttttccgtcc ttccgcggcc tcaaaccccc
    13141 cacacatgac cgtgcgagcg ctatctccgt gtcagggggc gcgtgggggc cgtgagagga
    13201 ccgggcgcgg gaaccctcgt gtcgcaggta tgccgcgtgg gcgtcgtggg agaggatggg
    13261 gcgttgatcc gccgacccac tcgcaggagc ccagcccggc atggcccgtc gcaccaccac
    13321 ccagcccccc gatggcgagg acgtcgtcga gcggatcgtc gacatcgacg tcgccgagga
    13381 gatgaaggcc gccttcctcg agtactccta cagcgtcatc tatgcccgag ccctgcccga
    13441 tgcccgcgac ggcctcaagc cggtgcaacg acggatcctc ttcgggatga gcgagttggg
    13501 gctgcggccc gagcgcccgc acgtgaagtc cagccggatc gtcggcgagg tgatgggtaa
    13561 gtaccacccg cacggtgaca gcgcgatcta tgacaccctc gtgcggctgg cccagccgtt
    13621 ctcgatgcgc ctgcccctgg tcgacgggca cggcaacttc ggctcgctcg atgacggccc
    13681 ggccgcctcg cgatacaccg aggcccggat ggcgcccgga gcaatgctca tggtggcggc
    13741 gctcgatgag gacaccgtcg acttcgtccc caactacgac gacacccaga cccagcccga
    13801 ggtcctgccc gcggcgttcc ccaacctgct cgtcaacggt gcgagcggca tcgccgtcgg
    13861 catggccacg agcatgccgc cgcacaacct cggcgaggtc atcggcgccg cgcgccacct
    13921 catcgagcac cccgacgcga ccctcgatga cctcatgcgt ttcgtccccg gccccgatct
    13981 gcccggcggc ggacggatcg tcgggttgga cggcatacgg gaggcgtatg ccggtgggcg
    14041 cgggaccttc cgcacgcgcg ccaccgcgcg gatcgagaac atcacccccc gccgccgcgg
    14101 gatcgtcgtc accgaactgc cctatctcgt cgggcccgag aaggtcatcg agaagatccg
    14161 cgaccaggtc cagagcaagc ggctgctggg gatcagcgac gtcaaggacc tcaccgaccg
    14221 cagcaacggg ctgcagttgg tcatcgaggt gaagagcggc ttcgtacccg aggcggtgct
    14281 tgaggagctc tatcgactga caccgatgga ggactcattc gggatcaaca acgttgcgct
    14341 cgtcgagggc cagccgcgca cgctcgggct cgtcgagttg ctgtcggtct tcgtcgactt
    14401 ccgaatcggc gtcgtccggc gacgcaccgc ccaccgactg gccaagcgcg aggagcgtct
    14461 gcacctggtc gatgggctcg tcgtcgccat cctcaacatc gacgaggtca tccagctcat
    14521 ccgggcagcc gatgacacgg cgacggcacg cgcccgcctc atgggtgtat tcgacctgtc
    14581 ggaggcgcaa gcgacctaca tccttgagct ccaactgcgc cggttgacga agttctcccg
    14641 gctggagttg gagaaggagc aggagcagtt gcgctcggag atcgaggccc tgcgggcggt
    14701 cctctccgat gagcgactgc tgcggcgcac ggtctccgac gagctggccg aggtcgccca
    14761 ggcgcacgcc accccgcggc gcacggtgct gctggagtcg gctggcgccg tcgccggggt
    14821 caccgccgcg gcccctgccc ggggccagag cgcggtcctc gagatcgccg acgacccgtg
    14881 ttgggtgctg ctctcctcaa ccggcctgtt ggcccgcacg acatcagccg acgatctccc
    14941 gaccggaggg gggcgggccc ggcacgatgt catcatcggc gcggtgcggg ccaccgcccg
    15001 aggcgaggtc ggtctcgtca cgagcctggg ccggatgctg cgcctctcgg ttcttgagtt
    15061 gccgacgctc cccccgaccg ccgactcccc ccacctgtcc ggcggtgctc cgctggcggc
    15121 atacctcgaa ctcggtcggg acgagcagcc ggtgaccctc gcctcccttg cggtcgattc
    15181 cccggtgatg gccctcggga cggcctcggg cgtcgtcaag cgcgtggtca ccgaccaccc
    15241 cgcccgaccg tcgtgggagc tcatcgccct gcgcgacggc gaccaggtgg tgggtgccgc
    15301 cccgacctcc agcgacgaga tcgacctggt gttcctcacc agtgacgcgc aactgctgcg
    15361 gttctccgcc ggaagcgtgc ggccgcaagg ccgagccgcc gcgggcatgg ccggaatccg
    15421 gctggatccc gcggccaggg cgctgttctt cggagtggtc gaccccactc gggacgggtt
    15481 ggtcgtcacc gcgagcggca cctcgggagc cctccccggc acccaggcgg gggccatcaa
    15541 ggtgactccg tatgccgagt acccggccaa ggggcgcggc acgggagggg tgcgagcgca
    15601 ccgattcctc aagggcgagg acaccttggt gctggcttgg gccggctcgg tgccggcgcg
    15661 ggcggccgca gccaacggag ggccggtcga cctgccggca gcgacgggtc ggcgcgacgg
    15721 gtccggcgcc acctggagcc atgtggtggc cgctatcgcc ggcccggcta gcggggccta
    15781 gtatctcgga tcgacctgag tctcggcgct cgacgcacct ctgcgatgac gtcggtgatg
    15841 acgtcgctga cggcgttgct cggcccgtgc gaaggcgcgg gctcgacgca taacaaggcg
    15901 ataaacaccg gccctgactc ttgacgggaa agcgtttaca cggcagagtc ggccatgccc
    15961 tcggacacgt ggggccgacc ggtcgggaga ccgtccgggc gtatcagtcg gttcatctat
    16021 ggggtgctct tcggaactct ccgtagcctc cacagacgcg cgggagcaag ccaccagcag
    16081 cggctacggg tagccgaagc tgtgttagtc attccgtaag tagacaaggg agtcaaacgt
    16141 atgcgcagga gtaccttcgc caaggcagcc acaggagcag ccgcgatcgc gctgctcacc
    16201 tcgggctgtt tgggctcgag tggcacgacg acgactgctg ccacgggcac ggccgcggga
    16261 gccggcaagg ccaccgtcga ggtgatgtac ggcttcggca acgagcagga gaccgccttc
    16321 cagaaggacc tcaaggcctt cgccgacaag agcggcttca cgatcaagtt caccaaggct
    16381 ggatcgtggg acaccgagat caagacccgc gtcgcgggtg ggaacccgcc ggacgtgggc
    16441 ctcttcccgc agcccggcgt gatgtgcgac ctggccaagc agggcaaggt cctcgcctat
    16501 gacgacgcaa ccgtcacctc cgccaaggcc accctggtca acggcttcgt cggcgccggc
    16561 acctgcaccg acggcaaggt ctacggcctg ccctccgcgg tcagcgtcaa gtcgctgctg
    16621 tggtacgacg cgccagtctt cgccgccgcc ggctacaagg ccccgacgac ccttgccgag
    16681 ctcgacgccc tgaccgagaa gatcaaggcc gacggcaaga ccccgtggtg catcgccgct
    16741 gagtccggtg gcgccaccgg ttggccgatc accgactgga tcgaggacct cgtcctgcgc
    16801 ttcgccggcc cggagaacta cgacaagtgg gtcgctggca cgctgaagtt caacagcccc
    16861 gagatcaagc cggcctggga ctacttccag aagatcgcct tcaccgaggg caacgtccgc
    16921 ggcggcacca aggccatcgc ggcgacgaac ttccagaccg gtggcaacgt cctgttcgac
    16981 acccccccgg cgtgccacct gttcaagcag gccacgttca tcgcgggcaa gggtggcttc
    17041 cctgacaccg tcctggccaa ggctgacacc gagatcggcg tcgtcgcctt cccggccaag
    17101 gaggctggca acaacccggt cctgaccggt ggcgacctgg cgaccgcgtt caacaacgac
    17161 gccaacacgc tcaagctgcg caacttcatc gccagcaagg acaacggcgt cgaggtcggc
    17221 aaggccggct acttctcgcc gcacaagtcc ttcgacgtca cgctctaccc gagcaagatg
    17281 ctgcagaaga tcgccagcga cgttctctac aagtcgagcg ccgctcgttt cgacgggtcg
    17341 gacctcatgc cggccaaggt cggcgcaggg accttctgga ccgagcccat caagtggatc
    17401 tccggtactc aggacctcga cgtcacgctg aacaacatcg acgcgagctg gccgaaggcc
    17461 gctagctgag gtgacaggac agctgcgggg atgacagcac gtcatccccg cagctgatcc
    17521 atacttcccg ccccatcact ccttcgtcag ggaggtcagg gactcttgac catcaaaatt
    17581 ctgaacgcct tgctcgccat catcggcggc atcggcggcg ccatgatctt gttctggttg
    17641 ctcaacaaac ttgccgaatc gctcaaaggc cgctgggaag accgggtcaa gccgtgggtc
    17701 ttcgccggcc ccgcgatcct cgccatcggc gtctacctca tctacccggc catcgtcacg
    17761 ctgcagtact cgttcgccaa cgaggacgcg acgaaatacg tcggcttcaa caactacaaa
    17821 gacctgttga ccgacgccac gttcctctcg gtgctgttca acaacctctt gtggatcatc
    17881 atcgttccgg ccctcaccgt ggctctcggc ctcggcgttg cggtcctcgc cgaccggctc
    17941 cggcccaagg gtgagaagct ctccaagacg gtcatcttcc ttccgatggc catcagcatg
    18001 gtcggtgccg ccacgatctg gcgctcgatc tacgagtacc agccgaaggg cagcccgcag
    18061 accggtctgc tcaacgccat catgggcgtg ttcggcaagg acccggtgta ctggtaccag
    18121 atcgagacgt ggcacctcaa cagcctgctg ttgatggtca tcctcatctg gacccaggtg
    18181 ggctactcca tggtgttgct ctcctcggcc atcaagggcg ttcctgagga caccatcgag
    18241 gctggccgga tcgacggcgc cggtgaaagg cgcatcttct tcagcatcgt cgttccccag
    18301 atctggccga ccgtggtcac ggtgttcatc accgtcctca tcggtgtcat gaaggtcttc
    18361 gacgtcgtgt acgtcacgac gaacggtgcg ttcaacaccg acgtcgtggg tcggcggttc
    18421 ttctacgaga tgttcaccaa ccagaacagc gggtatgccg cggccatcgt cgtgctcctg
    18481 atgatcgcca tcacccccgt gctcgtttac caggtgaggc agttccgggc tgaggaggcc
    18541 aaccgatgac caccgcagtt ccgccgagca ccgacgctca agtcattcgt cagagcaagg
    18601 ccggcagcgg cccaggcagt gcgacctggc cggtgcggat cttcgtcacc ttcctctgtc
    18661 tcatctggct gatccccgtc ctgggtctgc tcatcagctc gttccgcacg cgcgacgccc
    18721 agttcaactc cggttggtgg acggcgatcg ccaacccgct ggaattcacc cagtggaccg
    18781 tcaacaacta cgcccaggtg ctgacgacga acgactccgg catcaacatg ggccaggcgt
    18841 tcatcaacag cttcatcgtc acggtgccgg caacggtgat cccgatcctc atcgcggcgt
    18901 ttgcggccta tgccttcacc ttcatggagt ggaagggacg cgacctggtc ttcgtcatca
    18961 tcgtcggcct gctcgtcgtc cccaaccagg tggccctcgt gcccctgctg aagctctaca
    19021 gcagcctggg cctcaacggc accttcctcg cggtgtggct cgcccacatc ggcttcggta
    19081 tgccgttggc cgtctacatc ttgcgcaact acatgtcgtc cttgcccaag gccgtcatcg
    19141 agtcggcgaa gatcgacggt gccagccact tcacgacgtt ctggcggctc atcatcccga
    19201 tgtccacccc ggcgctggct tcgttcgcga tcttccagtt cctctgggtg tggaacgacc
    19261 tgctcatcgc tctgctcttc ctcggccgtg gtgagaacac catcgcgacc gtggccctgc
    19321 agggcttgca aggtcagacg ggtcagggcc gtgagctcat cccgtcggcg ggcttcatca
    19381 ccatcattgt cccgctgatg gtcttcctgt cgctgcagcg gtacttcatc cgcggtatga
    19441 cctccggcgc cgtcaagggc tgaggtcacc cgcaccatcg aggtcgagcc cgctccccca
    19501 ctgtggggtg cgggctcgac ttcttcgaga gcttcgtgaa agacgatgac gacgccgagc
    19561 catccgacag tgctgccgac acgcagcctg ttccgcgcca ccgaccgcgt agccgtcgag
    19621 atccgcgacc tgcgtcagtc ggcagagctg acggtgcacc gcgacggcgc cgtcctttcc
    19681 agccaccccg tctcgagtgc tggtgtcatt cctttgggtg agttggagcc ggggtcctac
    19741 ggcatctcgc tccgggccgg cgaccagcag atcgctcgct ccgcggtgca gatcgccggg
    19801 tctggtggcg accgaggcat cctgcgctac gcgttcgtcg ccgactatcg ccccgaccgc
    19861 agcgtggccg acctggccgc cgtcgccgac aatctccgtc gtctgcacat caccggcgtc
    19921 cagttctacg actgggccta ccgccacgcc gacctgctcg gcggtggcga acgttacgcc
    19981 gacgccctgg gtagccccgt cgacctcggc acggtccgcg ccctgatcgc cacgtgccac
    20041 gaggtgggcg ccgacgcgct cgggtatgcc gcggtctacg gagtcggcaa cgacgaatgg
    20101 ccggcttggg agtcgctcgc gatcaccgat gccgacggca cggcatacgg gctcggggac
    20161 tttctgcgac tggttgaccc gggcgccccc acctggctct accacttcgg caacgatcta
    20221 gcagaagcgt tggcgcagtg cggttttgac ggcttccacc tcgatcagta tggctaccca
    20281 aaggtggcac tgcgcacgga cggcacccgg gtcgacctca ccgagagctt cgtgcacctc
    20341 atcgaatcgg tccgcgagcg cgtgcctggc gcccggctgg tcttcaacaa cgtcaacgac
    20401 ttccccacct gggccagtgc tcaggcaccg caggacgccg tctacatcga ggtgtgggaa
    20461 ccccactcca cccacgcagc ccttgcgcag gtgatcggcc gcgcgcggtc cgtggccggc
    20521 gggcggccgg tcgtcatcgc ggcataccag caggtgtatg ccgtcgcccc ccgcgaggaa
    20581 gccgaccgcg cgaccgccct gacgatggcc acggtgttct cccacggcgc gacccacctg
    20641 ctggccgggg aggccgaccg gatcctcgtg gacccctact acgtccgcaa ccacgtggcg
    20701 cagccgggga ccatggacct gttggcgcgc tactacgact tcctcaccga gcaccacgac
    20761 ctgctcatcc catcggggat cgtcgaggtc accggctcct acgcggcctc ctacaacgat
    20821 gacctcgacg tctcgtatgc cgccgcgaca gtgagcggta cggccaggcc tggggtcgtg
    20881 tggcgccgga tcaccgcggt cggtgatgcc ctggtggtgc acctcatcaa cctctctggg
    20941 gccaccgact gccgctggga cgcaccgcag ggaccgccgg gaactcccgg ggcgggcact
    21001 ctgcggatgc ggcgcgtcgg gcccgacgtt ccggcggtgt atgccgccga cccggacgcg
    21061 agtggtcacc tggagccgct ggtggtcgcg gtggacggca cccacgccac cgtgcacctg
    21121 ccgccactgg cgacctggca gatgattgtc gtcaggtcca ggtgagagca atgcgactcg
    21181 catcgtccac ctacgcaagg atgccggagt gaccatcgtc gaccctgcgt gctcgcgggt
    21241 gtgggatacc ggtgcgctcc ccgcgtgggg cacggctgcg cgagcagact tcgtcgtggc
    21301 tctggagcag aacggtccgt ggggccgcgt cgcagccacc caatcgcacc tggacccggc
    21361 cttgggggcg gccctggatg cccacgtgtc cgggttgggc ggacgtctgc tgctcatccg
    21421 tcggcccggc cgccatgccg aacaggcccc tcagaccgac gccgaaccgg aggccagggg
    21481 cgccggtgcg ggcgcgggtg ccgatggggc cagaccgcag cagtcgaccc ggcgcacgta
    21541 catcgcgcgc acgggtccgg gcggatggct cgtgcgagcc gacctgaccg atccggcgat
    21601 gctgctgggc ctcggcgccg acgaactggc cagcgctggg gcgacggcag gccgcctggg
    21661 tggagccctc gacaccgtgc cactcctgct cgtctgcacc aacgggcgac gcgacgcgtg
    21721 ctgcgctcag cggggccggg tcgtcaccag cgcgttcgtc tccggcgggt tcgccacggc
    21781 cgccccggac caggtctggg agacgagcca taccggaggg caccgcttcg cgccgacggc
    21841 cgtgttgctg ccatggggtc gcgtgctggc ccgactcgac cccgagttgg cagccggtgc
    21901 cctcgacgcc gccgcttccg gcaggctggc accggcgctg ctcgggcggg tgcacgatcg
    21961 agggccgatg cacctccccc ctgcggagca ggccgccgat gcctggctgc gccggcagac
    22021 cgatcgccgc gacctcgacg atccgatgga tgatctgcgc gagcaggtgg acgtcgtggc
    22081 ccggcaggga cccatgctgc ccgagtcatg cggtaaggcc gccgtcccga cgatcgtcta
    22141 cgcgccgagc tggcgagcat gagacccgtc cgcaaacgac cagcccgcag aagaggggca
    22201 gccgggtgcc cagtccagcg ccgtcaaccc cgcgccgata tcgagcggga acatctcacc
    22261 ggaggcacgc catgactacc gccccgtccg tgccgaccat gctccacttg cgcctcggtg
    22321 ggacgagcgt ggtcgcgcgt ctggacgacg ccgccctgcc gtgcgtgctg cactggggac
    22381 ccgacctcgg cgacgtgtcg acccaggcgc tggatgaact gcagatcgcg ttgacacctc
    22441 cctacgtcga ctcggcgatc agctcgcaag cagcggtcgc gctcctgccg caacactcgt
    22501 cggggtggct cggccgacct gggctgctcg gcagccgagc cggcggacgc tcgtggtcgg
    22561 tgtccttcga tcgggtcgaa caccgcgtcg agaccgccgg gtcggcggac tgggcggacg
    22621 gcgccgccgg ggcggtgcgg ttgcgcagcg tcggcctgga ccggcccagc gccctcgaag
    22681 tcacgaccga gattgaggtg caccccagcg gattggtccg ggtccgtgcc caggtgcgca
    22741 acctcggcgc cgacgactac gaggtcgccc acctgttgcc cgctttgccg gtgccggcgg
    22801 aggcgggtga gctgctcgac atgaccggac ggcataccca tgagcggacc ccgcaacggc
    22861 ggcccttcga ccccggggcc tgggtgcggg aggcgtgggg cggacggccg ggccacgact
    22921 cggcgacggt catgtgtgcc ggtgagcccg gcttcggctt ccggtccgga cgggtctggg
    22981 gcgtccatgt ggcgcacagc ggtaaccagg tcgtcctggc cgagcactcg acggcgggct
    23041 ggcggctgct gcggggcggg gaggtcctcc ttccgggcga gggcgtccta cacccgcagg
    23101 agagctactc ctccccctgg ctggtggcct cctggggcga ggggttggac gccttctccc
    23161 agcgcgttca tcgcttcctg cgggcccggc cccaccaccc gcgtggcccg cgtcctgtcc
    23221 tgctcaacac gtgggaggcc gtctacttcg accacgacct gggcaaactg atcaccctgg
    23281 ccgaccgggc cgccgagctc ggcatcgagc gcttcgtcct ggacgacggg tggttccggc
    23341 atcgccgctc cgatcaggcc ggcttgggtg actggttcgt cgacgagggg gtctggcccg
    23401 acgggctgca cccgttggtt gaccacgtgc acgggctcgg catggacttc gggctgtggt
    23461 tcgagcccga gatgatcaac ctcgactccg atctcgcccg cgagcatccc gactgggtct
    23521 tccagaccga tcacgggccg ggcctgccgt cccgctacca gcacgtgctc gacctgggtc
    23581 acccggcggc atacgccttc gtcctcgagc ggatgagcac gctagtcggc gaataccgga
    23641 tcgccttcat caagtgggat cacaatcggc cgctcgtcga cgccgggcac tcccctgacg
    23701 gcagcccggg cgtccacatc cacaccgagg cggtctatcg cctcatggcc gagctcaagg
    23761 ctcgccaccc ggggctggag atcgagtcgt gctgcggcgg cggtggtcgg gtggacctgg
    23821 gcatcatcga acacaccgac cgggtctggg tgtcggactg tatcgacgcc cacgagcgtc
    23881 aacgtctggt gcgctggacc gggctgctgc tgccaccgga acttctcggc acacacgtcg
    23941 ggtcgggagc cgaccactcg acgcaccggc tgctgcacct gggcttccga gcgggcaccg
    24001 cgatgtgggg gcacctcggg gtcgagtggg acctcaccac ggcctcgtca gccgacctcg
    24061 acaccctggc tgcctggatc gccttccaca aggaggtccg tgagttgctg cacaccggcg
    24121 atgtcgtgca cgccgacctg cccaacccgg cgctactgct cgaaggagtc gtcgccacgg
    24181 accggtccga cgctctctac cgcctggccg ccctcgacct caccgtcacc tggccggccg
    24241 gccgggtccg tctgcctggg ctcgacccgg cgcgggacta ccacgtcagt gcccaggcac
    24301 ccggtgatgc ggcagccgcc actttcccac ccgggtgggg tccctcgggc gtcaccctgc
    24361 ccgggcgggt cctgcgtgag gtgggtatcc aggcgcccct gctcaatccc gaccaactgg
    24421 tgctgctgcg tgcacgtgcg ctgcctaccg ccgctgtgcc ggtctgatga gctccccacc
    24481 ggatcgctgg tcacctgcca tccgggtcag gccaacgcgc tcgaaggtcc ggctttgttg
    24541 caacctgtgg gttgagagta gattgacgag tagagcgcgc actcgcgcgc tttgtgtcaa
    24601 atccgggcag cctgttgcgg tgggttgttg caggggagtc aaccattctg cccgtctgcg
    24661 ggcgtcatca gacggaggtc ggcgtgcaac aaccgacaat cgctgatgtt gctcgcgcgg
    24721 ctggcgtgtc cgttgcgacc gtgtcgcgcg ccctacgcgg actggacaag gtgcacccag
    24781 agacccgcga acgcgtcgtc caagccgctg ctcagctcga ctacatcgcc agccccacgg
    24841 cctccggtct ggcctcagga cgcagccgcc tgatcgggat catcacgccc ttcatggcac
    24901 ggtggttctt caccggcatc atgagtgcca tcgagaagac ccttcgggag caccagcacc
    24961 acatcttgct catggacctg gagcgcagcg agacctccat gtcccgcctc tccctcaccc
    25021 aggggatgct cttcaagcgg gtcgacggcc tcatcgtcat caacgtcgag ctgcaggacg
    25081 ccgagcgtga cctcgtccgt cgcctcgggc tgcctgtggt cgccgtcggc agcccgttcg
    25141 aggactcccc gtgcgtgggc atcgacgaca tcggctcggc cgccctggcc gccgaacacc
    25201 tgttggcgct gggtcatcgc aagatcgcct atgtcggcag ggactacccc gaggcggcac
    25261 cgcgaaagac accgatcgac cggctggagg gtttccacca ggcgatgtcc cgagccggca
    25321 tcaacacccc gccgacttgg gtgctcgact ccgactggac cgctggagac tcgcgcgcct
    25381 cggccatggc cttgctgtct ggtcccgacc ggccgaccgc ggtcctcgcc gcctccgacg
    25441 agatggccct cggtgtgtgg ggcgcagccc gccaactcgg tctggaggtg ccgagggacc
    25501 tgtccatcgt cgggatcgac gaccacgagt tggcccccgt attcggcctc accaccgtgc
    25561 gtcaggatgt cgccgcccag ggagtcgcag cggccagcac cttgctcgcc cttctcgggc
    25621 tctatgaccc ctcagcgatc cctgaccaga ccttcccggt cgaactcgtc atccgcgggt
    25681 cgaccacagc ccctgcgaag ccctagctca acctagccac cgctgggggg acacccgccc
    25741 ggagccgcgc gagcggcata ctcaccaggg tttggccgcc gggttagccg aaccgccccc
    25801 acccgccggg tttccttgcc cgtccggccc gttctgctgg cggtcctgct ggttctcatg
    25861 ggtctgctgg tcgagttcat ccgccttgcg ctgctgctcg gcctgctgct gcgcatcgct
    25921 cgactggtca acgccggagg actgctgggg gttgagcttc tgttcggccc ggtcaccggc
    25981 cgcttcggcc ttctgcccgg ccccgtccgc cgctggcggg aaacaccccg gaggcgccgc
    26041 atcggcgacc gccttggtgc gggtccagta gtccttcgcc gcgacctcgt cgccggccgc
    26101 cttggcggca tcgcccagtt tctccagcgc cagcgccaga ttgacccgga tctgacacga
    26161 gtcgatcgag tcgggcggcg ccaactccaa cgcctgctcg aactgcgccc gcgcgctgtc
    26221 cagatcgccg gccagcacga aggcgtcgcc gcgcgcgaag ggagccttgt gccgctcgac
    26281 aacgttgagg aacgacatcc gatcagccgc ctcgaagacc gtgtgcgctg ccgcggcgaa
    26341 gaagccgtca actgcctgtg tggcggcggc gctcatcgtc agcagtttga cggccacaag
    26401 gatggccacg aacgcgggcc cgatcgacca actgatcagg cgccgccgca tggcgagccg
    26461 cgcgtgtcgg gcggccgccg gttcgtccgg atcgagggct gcggggcgcg ccggcgccgg
    26521 gtggggagcg agcgtcatga ccgcaccttc tcctcgtggc cgtcccgatc tgccggtatg
    26581 ccgccatcct ccggtatgcc gcgtgctcgc ctcgggcggc gcagccccca caaccgggcc
    26641 agagccagcc cgacctccca ggccacaagc ccggcaaccg ccagcagggc gatccagtag
    26701 aactcctcac ggcccgccga cagcggcccg tcctgtgcca cggtcacccg atcgaggccg
    26761 gtgagatcca ccagccccag gacggcgtcg acactctcac ccggcgagcg atgcaggtag
    26821 gggaccccca attgttgggc gatcgagccc aattggcctt cgtcgatgac cgagaccgcc
    26881 ggcttgccgg tgctcgggtc gagcaaccag ccggcatccg cacgcgaccc gggcactccg
    26941 gtctcgtgca tctggcctcc gccggtcgtg ccataaccca ggaccgcacc gccgttgacc
    27001 aggctctggt ccactcccag cgctgccggt gggctgccgg acgtctgttc accgtcgccg
    27061 aggtagaaga ccgcccgcgc ccgctccgga tgcgcggcgg cgacccgggc cagggtctgc
    27121 tgcagcaacg gtccggccgc ggtcacgctg ctcccccgtg accaggcgga cgtttccggc
    27181 agcatcgtct cagccgcggc ggcgacggcc gcgccgtcgg aggtgagcgg catacgaagg
    27241 actgcggcct ggtcgaaggt gatgagcgcg aaccgcgccc ccgccattcg ccctgagatg
    27301 gccgtgatgt ccgcgcgcat ccccgacatc cgcgggtcgg tgccgaagtc ctcggccatc
    27361 gacgacgtgg tcgtgtcgac gacgaagaac acgttgagct cggacgcgct ggcccgcacc
    27421 tcggcaccgg gcaggcccgg gcgcagcgag gccacgagca ccgcggccac gagcagggtg
    27481 cggcgcactc gcgttcgcag actcggctgg gggccgggct gcggccacca caccagcgcc
    27541 aacgccacca ccaccgccag caccaggacg ccgacagaca tgaccggaag aagagtcatc
    27601 ggcgccaccg tcgcagggcc ccgagcaggc caagcacccc gagccaggtc cacccgaccc
    27661 acgacgccgg ttggtcgtgg acgagcgtga tcggtgctcc ggggatccgg gaggcctccc
    27721 gagcagagac ggcggacacg atccggctca ccgcgtcgac gctgttcaag gggtagtaga
    27781 gcccaccgct gccctcggcg accgagcgca tctcggtcgc cgtcgaactc cccgcgcgat
    27841 gagccgggtt gaggccgtag atctggatgc cggcagcttt ggccatcgtt ccgccttgcg
    27901 ccaggctgaa gatcgacctc cccgcggcga gattgtcggt cgcgaggatg atcgaccggg
    27961 cccgggtcag gttgccgtgg tcgaagctgc gcacacaggt ggccaagccg tcaccgatga
    28021 gcgaactccc ctgcccgttg agtgtcccgg cgtagaactc gtggtcgaag ctcccctcga
    28081 agacccgctg ggcgtcggac aactgctcct cgacgaagtc gtagtcgtcg gtcaggggaa
    28141 acaccggcac ggccgaggag ttgaagatga ccagcgagag acgctccccc ttgaactcct
    28201 tggcaagcga tcggaacgtc tccgccacct cggcgtcggc agtagtcatc gagcccgaga
    28261 cgtccagaca gaggacgatg tcgcggttgg ccttctcagg gttgatgacg gtcgtggcca
    28321 cgggccgggc cgcgccgatg atcgcgagca cggcgacgaa acccgccgcg gcggcataca
    28381 ggaccgtcat cagtcggtgt cgccagatcg cgtggacata ctccggaagg ctggtgagca
    28441 gatcggtgtt ggccaacggc agcgccgccg gcggcctgcg acggcgcagc gcgaaccaca
    28501 ccagcgcggc aatcgcgaga gcccccaggg ccgcccacgg caggatccac ggttgtttca
    28561 gtgccattgg gccaccaccg ccttggccgc ttcggcggca ggccggatgg gtcgcgccgg
    28621 gtcaggcccg aactcgccgg ggtagagctg caggacgacg tgtgtcaccg gagccagccc
    28681 cgggccgcgg gagccgaggt cagacagggt catccgcgac gtggtcgccc ccgacacgtc
    28741 atcgatgaag gcccgcaccg cggcactgag ctcacggtgg gacgtccggg cgtcgaccct
    28801 gccctgctcg cattcccaga cgatctggtc gatccgggtc atcgtgtcgt gcttcgcgcg
    28861 ggcaagccgc caccccgggt ggatgggaac gggcccggcc ggggttgtgg gccggacgcg
    28921 acgggtgcgc caccagatga acccggccca ggcaagcacg gccaagatcg ccacccaggc
    28981 gagcagcgtc cagacccagt ggtaggtcat cggcgggtag aacgggtcgt caacgtgcac
    29041 ggcggcgctg cctctccagg agcgcgaaca tgctgcccac gacctccgac gtgccaccca
    29101 cgcgagcgcg cgcgatcccg agccgccgca gccgctgatc ggtcgcctcg cgtcggcccg
    29161 cgagagcctg ctggtatgcc gcctggagac gttcgccgcc ccgcaccgcc cctgggagag
    29221 cgctgccgtc ggtgacctcg gaggcatcgg cgcgcgggtc gaaacgcgtc gggtcggcgt
    29281 cggccacaac cacccagagc acctcgtgtt gagcgtgcag tcgccgcaac aacgggtcgt
    29341 cgtcagagcc caggccgcca tcgtcggtca ctgccacgat gagcatccgt cgccggacgt
    29401 gctcggcgag gtagtgcagt tgcgcagaga accggctggg cggagcctca agcgtgctcg
    29461 cgcgatcgat gacccgcagc agacgctcga ggttggcctc ccccgcagcc ggctcgagca
    29521 tcctggtgct ctcaccgtcg ccgctgatga ggccgaccag gtcgccgtgg cggacggcga
    29581 gatagccgag catgccggcg atctggatcg cgatgtcctt cttcggctcg cccgacggcg
    29641 ccatggccgc catcgagcga ccggtgtcga ccagcagcag gacggtctgc ttacgggtgg
    29701 cgacgtagcg tttgaccagc ggggccccgt gccgagcggt cgccttccag tcgatgtcgc
    29761 ggatctcgtc gccggggatg tattcgcgca ggtcgtcgta gtcgaggctg cgccctcgga
    29821 agaccgaggc atactccccg tcgagcaagc cccacgtgcg ccggtgcgcg actatgaaga
    29881 gtttgctctt cactcgggtg agcagggcgg ccacgggtgc tctcgtctgg tcggggcggc
    29941 tagcgcaggt atgccggtcg gcccggtcag ggggtgcgga ccgcggtgat gatggcgtcg
    30001 atgacccgct cgaccgggac atcgtcggcg acggcctcga agccaaggat gatccggtgg
    30061 cggagcactc gatgggccag ggcctggacg tcgtcgggca cggcatggtc gcggccggcc
    30121 aacagggccc gcgcacgggc ggcctgggcg aaggcgatcg acgcccgggg gctcgcaccg
    30181 aactcgacgt accgggccag cgccgggtcg aggtaccgct cggcgtgccg ggtgacgtaa
    30241 gccagcccca cggcataccg gatgacggtg tcatcgaggt agaccgcacg ggtcaacgcc
    30301 tgcagtcggc gcacgtcggc gaggctgacc gcgggggtct gctcggcgag atagatgccg
    30361 gcgtcgatcc gccggaccac ctcggcctct tcgtcgaggg tcgggtaatc caggacgtcc
    30421 ttgagcatga agcggtccag ttgcgcctcg ggcaggacgt aggtgccttc ctgctcgatg
    30481 gggttctggg tggccaggac gaggaacggc tcaggcagcg ggtagacctg acccccgatc
    30541 gaggtctgcc gctcctgcat ggcttcgagc atcgccgact gggtcttggc ggagctgcgg
    30601 ttgatctcgt caagcaggac gaggttggcg tggaccggac ccagccgggt ctcgaactgg
    30661 gtggtgcgca cgtcgtagac ctgggtgccc acgatgtcgc tggggagcag gtcgggagtg
    30721 cactggattc gtttgaacga gccgccgacg gtgcgcgcga gggtggctgc cgcggtcgtc
    30781 ttggccaggc cgggcaccga ttcgagcagg atgtggccgc cggtgagcag cccgagcagc
    30841 agggtctcgt gcagtcgcac ctggccgacg acgcgctcct ggaacgagcg tgagatggtg
    30901 gcgacggtcg agcgcgccca ggcgagatcc tccggcggga tcgccgtgac gcccggtcgc
    30961 ggggtggtcg acgcactggt ggggtatgcc gcctgcgctc ccggcggtgg tcccgggtcg
    31021 atcgtcgtgc tcatgctcac tccccctgtc gatgctgctc acccgccgac tcaggtcggg
    31081 tccggcccat ggtagacgtc tgatgcggcg ccgacgttcc gtcccggcgc tcgccggtcg
    31141 gtgcgtggcc gcgtgatccc aggtaggtca ggcatcgatg cgttcgcggt cgagggtctt
    31201 ggccgactcg acgatgaagt ccttgcgcgg cgccacgtcg ttgcccatga gcaggtcgaa
    31261 gacaccttcg gcgatccggg cggcctcctc ggcctcgccg agggtgaccc gacgcagcgt
    31321 ccggaagcgc gggtccatgg ttgtgtcggc caactggtcg gcgtccatct caccgagccc
    31381 cttgtagcgc tggggcggct ccttgacctt gcgccccttg cgttccagcg ccgcccgcgt
    31441 cgagcgcatc tgcgcctcgt tgtaggtgta gacgtattcg ttcttgcgcc ctgggccgcc
    31501 gatgacttcg aggcgatgca gcggtggcat cgcggcatac acccgtccgg cggccacgag
    31561 cgggcgcatg tatttgaaga acaaggtgag cagcagggtg cggatgtgag caccgtcgac
    31621 atcggcgtcg ctcatgatga tgaccttgcc gtagcgggcc gcctcgagat cgaaggaccg
    31681 ccccgacccg gccccgatga cctggatgat cgccgcgcac tcggtgttct tcaggacgtc
    31741 ggcgaccgag gccttctgga cgttgaggat cttgcctcgg atcggcagca gcgcttggta
    31801 atccgaggaa cgcgccaact tggcggtgcc gagggcgctg tcgccctcga cgatgaacaa
    31861 ctcggaccgc agcacgtcgt gggtgcggca gtcagcgagt ttggccggca gggtggaaga
    31921 ctccaaggcg gtcttgcgac gctgggtctc cttgtgctgg cgcgcactga tccgcgcctt
    31981 catctcggag acgaccttct ccaacaacag cgccgcatga gccttgtcgt cgcgcttggt
    32041 cgaggtgagg cgggcgttga gctcctgctc aagcaccttg gcgaccacgg ctcggaccgc
    32101 cgcggtgccg aggacctcct tggtctgacc ctcgaactgc ggctcggcca gccggacggt
    32161 gacgactgcg gtcaacccgg cgaggatgtc gtccttctcg accttgtcgg tgccgacctt
    32221 gagccggcgg gcgttgagct ccagttgacg gcggaacacc ttgagcaggg actgctcgaa
    32281 acccgccaca tgggtgccgc ccttgggcgt ggcgatgatg ttgacgaagg agctgacccg
    32341 cgagtcgtag ccggtgcccc accgcatcgc gatgtcgacg gagcactccc gctcgacctc
    32401 ggtcgggctc atatggccgc gctcgtcgag caccggcacg gtttcggtga agttcccggt
    32461 gccctggagg cgccagaccg cagtgagcgg cgggtcggga gcgaggaact cggcgaactc
    32521 cgagatgccg ccgaggtgtt ggaagacctg ctcgtgggtg ccctgggcgc caggagtgtc
    32581 cggtgctccc cgctcgtcgc gcacgaccaa ggccagcccg ggcacgagga acgaggtctg
    32641 tcgggcgcga gcggccaaca ggtcgtcgtc gaggcgggcc tccttgagga agatctgcag
    32701 gtccggccag aagcggatcc ggctgccggt ggcgccccgg ggcgccttcc cgacgacgcg
    32761 cagctccgac cccgtctcgt atgccgtgaa cggcgcctcc gggctgtgcg gacccggcgg
    32821 gtcggcgaat acgccgggct caccgcggcg gaagctcatc gcataggtct tgccaccccg
    32881 gtcgacctcg acgtccagcc gcgaggacag ggcgttgacg acggatgcgc ccacgccgtg
    32941 caggccaccg gaggcggcat acgaaccgcc gccgaacttg ccccccgcgt gcagcttggt
    33001 gaagaccaac tcgacacccg agagcccgga ccggggctcg gtgtccacgg gaatgccgcg
    33061 gccgttgtcg cggacctcga tcgacccgtc gccaccgagg atgatctcga tacggtcgcc
    33121 gacgccggcc agcgcttcgt cgactgcgtt gtcgatgatc tcccagaggc agtgcatcaa
    33181 cccgcgctgg tcggtggaac cgatatacat ccctgggcgt ttgcggaccg cttccaggcc
    33241 ttcgagcacc tgaaggtggc gggcggtgta gtcccctccg cccgcgccgg agccactcgc
    33301 ggcggtgcgg ccggtcgtgc tgcgggtggc cactggatgt cctctctgcg gtgcgttgat
    33361 gttcggcgac ggcgtgccac acgcagggta gtcgaggcgt ccgacagccg ggtccggggc
    33421 gcgcgggcgt gtctcagatg ggtatcaata ccgcgcggga acgaaccggc gtgcttgact
    33481 gttgggactg acgtgggggg attcagcaac cagcaagcgt tgctgtccct gcacgtcgaa
    33541 tcagcaacac cgggtcggca cctgtcggcc cgcacgagag agatgagaag gcagacgtga
    33601 acgccacctt ggtccaccgc ttgagcgccg cagatcgctg cgaccgctgc ggcgcacggg
    33661 cctacattcg cgcccgccta cattccggcg gggagctgta tttctgcgcc caccacggac
    33721 gccagcacct tccccagttg gccgatcagg ccgttgagat ccacgacgag actgagctgt
    33781 tgaccgagac cgcggacgag agcaccggcc tgcggtgagc ttcgccgacc cggtcagcct
    33841 cacgatcgtc acggggctgc tgatggttgt cgggttggtc ggcattgtcg tcccggtcgt
    33901 cccggggttg ctgcttaccg tcgcggcgac cttgttgtgg gcgtatgccc accccgcgcc
    33961 cggcgcctgg gtggtcttct gggtcgcagt cgtcctgtat gccgcgggcg tcgtcacgca
    34021 atacctcctg cctgggcgca agatgcgccg tgagggcgtg ggcacctcca cgatgctgct
    34081 cgcggtgctc ctggggatcg tcggcttctt cgtcatcccc gtcgtcggcg ccctcgtcgg
    34141 gttcgtcctg gggatcttcc tcgttgagtt gacccgtcgt caggatcgct ctgcggcgtg
    34201 gacgtcgacc aaggcggcgc tggtcgccgt gttgcactcg atgggcatcg agttgctggc
    34261 cggcctggcc atcctgctga cgtggatcat cgggctcatc gtcctcggcc ccggctgagc
    34321 gatcgcgcag ccagcgttca cctgtcgggc cgctgctcgg agctggcgtt caccactggt
    34381 cgcggtcgcg acgctcccag cgctcttcga gcatgttcat gaagccctgc cccgacttgc
    34441 gggccttggc ggtccgggca gccgtggagc cgtcaccctg gaccagaccg accgggccag
    34501 tcttgcgggc tggtgcgaac gcccacacac cgccggcgac catgaccgcg aaaccgaccg
    34561 caccgagcca gatcgcctga cccaggacgc cgaggaacac cagcccgagg ccggcgagga
    34621 cggcgaggat gccgacgagc atgcgtcgct tggtgccccg gtcgccatgg ctgcgaatgc
    34681 gcgtggcaaa gcgagggtcc tcggcgtaga gcgcctgctc catctgttcg agaagcttct
    34741 gctcccgctc cgacagtgcc atgacccacc cctcccaggg cttccagcgt ttccgaggcc
    34801 aaacccggcc atcatccgga caacgatgcc atccgacccg tggttccggg tcaggacatc
    34861 aggataggtc cggatccggg gttgtggtag ccggagcacc tgccggttgg cccgggccga
    34921 cgcttcgagc gctattcgcc ccctcgcggc atactccccg ggtgggcgac cgactccccc
    34981 cggacgagcg ccgcgggccg caccgcgcct gccccgaacc gggcactggc ccggtcgacg
    35041 gcgcggtcgg cgtcgcgcca gccgaagtcc gggtcgtcca gccggggttg gacggcggcc
    35101 cgctcgctct cgaccaaccc ctccattcgc acgccgacca ggcggatccg ggcgcgctgc
    35161 aggccgaggg cgtcgaacag cgccgtcgcg gcggcataga tctctcggga cacgtcggtg
    35221 gggtcgcgca gggtgcggct gcgggtgatc gtcgtgaagt cggcgaaccg caccttgagg
    35281 gtgacggtgc gtcccatcag gccggctgaa cgcacccggg ctgcggtccg atcggccaag
    35341 cgcagcaact cgcggtggat gagcaccgga tcgtcgatgt cctgggcgaa ggtctcgtcg
    35401 gagccgacgc tcttctcccg ctgctgaggg aggacccggc gctcgtcgcg gccccaggcc
    35461 agcgcgtgca gatgggtgcc ggtggcatca cccagggcgc gacgcagggt gtccaccggc
    35521 gtgtgggcga gatcggcgac agtgtgcagc cccagccgtt gcagggcctc ctcggtgcgg
    35581 tcccccaccc cccagagcgc accgaccggc aactggtgca ggaaggccag cacctcgtcc
    35641 tgcgggacaa cgagcagccc gtcgggtttg gccaggccgg aggcgagctt ggcgacgaac
    35701 ttcgtcgagg ccacgccgac cgagcaggtg atgctttgct cgtcggcaat ggtgtcgcgc
    35761 agccactggg cgatgctcgt cggcgggcca aggcgccgga ccgccccggc gatgtcgagg
    35821 aacgcctcgt ccaacgagag cggctcgtag cggtcggtca ccgacgcgaa ggtggccatg
    35881 acggcgtcgg agatcgcggc ataccggtca tggttggggg cgatgacggt ggcctggggg
    35941 cacagccgcc gggcgcgggt catcggcatc gcggaggtga ccccgaaggc gcgcgcttcg
    36001 taggtggctg agaggacgac cgagcggccg ccgccgccga tgatgaccgg ggtgccgcgc
    36061 aactcgggtc ggtcgatgag cgacgcggag gcatagaacg cgtccatgtc gacgtgcagg
    36121 acggtgcacc cggtgtcgtc gggagggccg tcgccggtgc gcggcggcgg gcggaactgg
    36181 cggcggctca tcggccggct cccaggccgg gatcagcccc ggcgggcgag caggtgcagg
    36241 gccgcaccga gctcaccgag gaaggggtat gccgggtgcc cggcgagcgc ctcctcgagg
    36301 gcgacgaggg cggcccgctc ggcgtcggtg tcgatggatg tcgacggcac caggtcggcg
    36361 agcaggcgca cgccgtgcca gtgctcgacc tgcagcccgg ctgattcgac gagggcgcgc
    36421 agctgggcca ggtcgaagcg gcgcggcaac gggtcgtgag caccccagcg gccgtcgggg
    36481 ctggtgagag cggtgtgggc ctggccgaac tccccggcca gggcccgggc ccagacggtg
    36541 gccagccggc cggcgacgag caacgagagg tggccgtcgt cggcgagggc gtcggcaagg
    36601 actcgcaagg tggcggtggg gtcatcgacg acctcaagaa cgccatggca gcagaccaag
    36661 tcgaaggccc gctcgggcag caggtcggcg agggtgtcgg cgtctccctg gctgccgtgg
    36721 acccgggcct ggacgccgga ctcggcggcc cgccggccca gggccgcgag cgcgtcgggg
    36781 ctggggtcga cgacggtgac gtcggcgacc aggtcggggc ccaactcggc aagcgggacg
    36841 gccaattcgc cggtgccgcc gccgaggtcg agcacccgca acggacggcc gagggcggcc
    36901 gcgcgctcgg tgacgaggtc tttgacggca gcccatagag ccgcggtgcg cacgccggtg
    36961 gcggcgcggc gggctcggcg ctgctgctcc tcggacacgc atccacccta gtgcccactg
    37021 ctgcccgggc tggagtgcca gagcttgcgc ggcggtcgcc cggcgccgct cccctgaccc
    37081 gccgcgtcga tggcatcgaa ggcgtcgggt gtgacctggt ctgtgaggcc ggcatggtcg
    37141 acagcgccag ccaggtgtcg ggcggacttg acgtcctcac ccggtggtcg ggtgtcggca
    37201 tacggcgatt gtttgaagcc cgaggcgtgc acgaggacct ggcgcggtgc cccaggcgcc
    37261 ccgaccgggc tcgtgggtga ccccccgccc atgccaccgg cccggctggc cggtcgaccg
    37321 ccgacaaggg acgcggcgtg gtccaggcgt gcagcccgct cccgatcggc gtatgccgtg
    37381 tcgtcccgat ccccgaccgg gctgccgtcc gcagcggctg cggcagcatc ggcggccggc
    37441 tcggcccacc cggtgccgcg ggggacggca tacgggagtg cgtaggcggc cgcagcggca
    37501 gccgcgcggt catgggcggc ggcgtcagca gcggcgagtg cgtcgagcag ccgtgggcgc
    37561 ccgccggtgg cgaacgcctc ccagagccca gacagctccc aggccccggt ggcccgcagc
    37621 gagatgcctc gggcgccggt gcgtcggacg actccgcgca cgaggagcag ccacgagtgg
    37681 aacaccgtgg cggcatacgg gccctggacg tcctcgaaga acgtcgagtc ggacgggccg
    37741 gtgccgtcgt cgagggtgag gaagacgacc cgccgaccgg agcggatcgg tggggtctgg
    37801 gtggcgacct tgaccccggc gacccacacc tcgctgcggt tgcgggcgct cagcaggtcg
    37861 cgggagcggg tcaccccgag tgcctcgagc atcggctcgt agtgggcgac gacgtgggcg
    37921 ctggcgtcca gcccgaggat ctccaactcg gcgcgcaccc gctcggggcc ggagagctcg
    37981 ggcaggccgc tgccgcgggt gagagtgggc gtgtcgccga ggggcaggac catctgggta
    38041 tgccggtcgc tcacgtgcac gggcgccgcc gcctgggact gggcggccgc aagcgcggcg
    38101 acgtcgaagg cccggatggt gccggggccg gcgggcaccc cgggctgctg gacgaggacc
    38161 gctacctcgc gccgggggtg ggcggcggcc agggcccggg tgtcggtcac cggagcaccg
    38221 gccgcgtcac cgcccggacc ggtcgccgac atcgtcgccg ggcgacgggt gaaccgggag
    38281 cggccacccc ggagcgcctt gccctgaccg ctgccgcccg accgcaccca ccggtcgagc
    38341 tcggcgacgt gcaggagcag gtcgcggcgg gtcacccgct ctcggacgcc gagcccgcca
    38401 gcgccgaagg accgatcggc cccggcgccc gtggcggtcg agatgccgta gaccgagtcc
    38461 aaaccaccgg caagcaccag ccgctccatg atggggcggc tgacctgggc ccggtgggcg
    38521 aggtcggcca ggtcggtgaa cggctggccg gcgacgatcc gggtcacctc ggcctcggtg
    38581 atgcctttga cgtcggtgag ggacacccgg atgccgtacc cccgcccatc aggcagatcc
    38641 gggtcccact ctttgccggg ttccctgccg ggtcgaggta ttccgccgtc agcagacgat
    38701 ggcgaaatac ctcgactcgg cagggggtgc ggcggacgac ccggcagggg gtgcggcagg
    38761 cgctccacgg cataccaact tttgctggcg ttgacgtcca ggccgaggat ctcgatgccc
    38821 agggagcggg cgtcgtcgag gatgagccgc ttgggataca tcccggggtc gtgggtgagc
    38881 accccggcga ggaaggccgc cgggtggtgg gtcttgagcc aagccgattg ataggtcggc
    38941 agcgcgaacg ccgccgcatg cgccttgcag aagccaaacg acgcgaaggc gcgcagcacc
    39001 tcccagatcc ggtcggcgtc ggccaccgta taaccccgag cgagcgccgc cggccgccac
    39061 caggcctcga cgtcgagctg accttgaggt gagccgagcg cacggcggac ctcgtcggcc
    39121 tgggccaggc tcactcccgt cgtctcggcg acgatccgca gcacctgctc gtggaagacg
    39181 acgacgccgg cggtctcggc cagcgccggg atgagcgtgg ggtgcaggta ttccggctcg
    39241 gcccacccat gccgggcctt gaggaacggc acgatcatgt cggacttcac cggcccagga
    39301 cggaagagcg agatgtcgat gacgaggtcc tcgaaccgct ccggcccgaa cttaccgatg
    39361 agctcgcgct ggcccgggct ctcgatctgg aaacacccga gggtgcgcgt ggtgcggatc
    39421 atcgcgaagg tcgcctcgtc gtcgaggggc acctgggcac ggtcgtccag atccaccggt
    39481 atgccgtcca cccgcgccac ctcgtcgacg gccagggcca tcgccgactg catccggatc
    39541 ccgaggacgt cgagtttgag cagccccatg acctcgacgt cgtccttgtc gaactgactc
    39601 atcgggaagc cgatccagct ggcctcgacc ggggtccggt ccagcagggc ggcgttggac
    39661 aggatcaccc cgcacgggtg cagggcgatg tgccggggca gcccgtcgag gcgctcgacg
    39721 aggtcccaca tgaggtcgta gcgggccgcc ccgagcccgg agacccgcaa ctcgggcagg
    39781 tcggcgatcg ccgaccgcgc gtgccgggcc gagatgtgcg ggaacgcctt ggccatcgcg
    39841 tcgacctcgc cgggcggcag gcccagcgcc gccccgacgt cgcggatcgc gtggcgcacg
    39901 cgatagctgt ccatcatcga cacacaggtc acccggtcgc cgccgaaccg ggcgaggatc
    39961 ttttcgtaga tctcggtgcg ccgggccgac tcgacgtcga tgtcgatgtc gggcagttgg
    40021 gcccgcaacg gcgagcagaa ccgctccatg agcaggtcat aacggatcgg gtcgaccccg
    40081 gagatgccca gcaggtagtt gaccaggctg ccggcccccg agccgcgggc cgcgacccgc
    40141 acccccatgt cgcggatgag gtcggtaacg gtggcgacgg taaggaagta ggtcgggtaa
    40201 cccaacgccg cgatgacctc caactcctcg tcgagccggg cggagatccg ggccacctcg
    40261 ctgtcggtgg ccccgggata gcgggcgccg acggcccccc ggcagcgctg cacgaggtgg
    40321 tgttgcgggt cgtcgtgcag gcccaggccc agagtgctct gctcgggcag gtgcaccccg
    40381 ccgatcccca ggtcgatcgc cgggtccagg acgcactcgc tggccagggc gacggtggcg
    40441 gcgagcaggt cgcgggcgcc gtcgcggacc gtgctcgtgc ccccggccag ccccggcccg
    40501 gaggcctgga cgatgtcgca ggcgacggca tacatcgcgg gggtgggggc caggtggccc
    40561 gcggtggtga cccggtcgag gtgtcgcaca tcgagggcga ccaggcggcg ggcggcatcg
    40621 aggacatcga cggtcgccgc ctcggccggg tcggcatggc ggacctgcgc cgagaggatg
    40681 acggggacgc cgaggtcgcg gccgagggca agcatccggg cggcatgccc gaggctcgcc
    40741 ggggtcccct ccggaccacc gtggcacacg gtctccaggg ccagggcgcc ggccggcagg
    40801 accgcgcgcc agcgctccag cgcctggtat gccgccgcgc gccgcttggc caggaccgcc
    40861 cggccgacgt cggagtcggg tccgagcagg acgatcaggg ccgccgggtc gtcgccgaca
    40921 ggagccgcca actcggcgag cacctgcggg gtcgtcagcg ggttgccccg ctcaccggac
    40981 aagtgggtgc gggtgaccag gcgacagagc cgggcccagc ccagccccgg ggcgactccc
    41041 ccggccaggc cgcgggccag cacggtcacc cgaggcagcc gggggtcgac gatcgcgccg
    41101 ccgcgcacgg gggtccggac cggcgccgac gggcgggccc agaccggtgg cgcggttgac
    41161 gacgtggacg cggaggccga gccggtcgag gagacagagg aggcggagga gaacgagccg
    41221 gggcgggcgg cataccggcc ggtggcgggg tccgacggcg ggaggtggac ggcgaggtcg
    41281 actccgagga tgggcgcgat cttcgcctcg gtgcaggcct ggacgaaccg caccgcgcca
    41341 tagagcccgt cgcggtcggt gagggcgagc gccggttgcc cgaaggccgc cgcccgggcg
    41401 acgagggctg cgggggtcga ggcgccgtac tgcatggaat agcccgaggc gacgtggaga
    41461 tggacgaagg tcgacatggc aggacgcggg tggcagacga aggagcaggg ctaggtcaac
    41521 ggccggcatg ccgcgcgagc ggcgtcacgg gggcgaggta gtccgggcgc acgaaggtcc
    41581 tcgggacgcc cagggcgtcc tggacgacct cgatgaaggc ctcggcctgg cgcaccaggt
    41641 cgtcggcctc ccgagccggc aggacccggt cgccgcgctc gacggccgcc cgctgccggg
    41701 ccgaggcggc gaagaagacc gcccactcgg tcagctcggg ggccagcgtc ggcagcacct
    41761 cccacacgct gcgtggccgg gacgagcgcg ccggggtggt ccgggcggcc agcaccgcag
    41821 cggccgcccg caacccggcc aggtgagcca ggacatagcg ctcccccacc tggtcggcct
    41881 ggctcgcata cagcaggctc gcctgggccc ggtcgagcaa ctccatgacg gtcgccgacg
    41941 gcgggacgtg ccgtctgggt cgcaacggtg ggctcggcgt ggccgggtag ggatgcgacg
    42001 gtgctgcgct catgtccggt tctccttcgg tcggctcgtc atcggtcggt atgccgcgat
    42061 tcctcggtat gccgcgcgca ctagtcgctc acccgcagca gcagccagcc atctgggtcg
    42121 gggtcgcccg ggctggtctc gtcacaggcc aggtcgaaga cgccggtgcg ggtgagccac
    42181 cccgggccgg cctcgacccg ccagacgaga cgctcgcgag cagcgacggc gatggccgac
    42241 cggcagtcgg tgtccgagct agtgtttgag gcaggggtcg atgccgtgtt tgaggccggg
    42301 cccgatgacg tggcctcgag cgtggggtcg gcgtcgagca cctcccgcca ccaggcccga
    42361 cgctcgcgcc agtgggccag gatcgcgcgg acgtgatagc gacggcccgc ccactcgaac
    42421 cacgtcggcg acgacgcggc ataccctccc gggccgggag gcacctgctc gaggtcggcg
    42481 agcgagtcag gggtcagccg cgacgcggtc ccgacacgga ccggctcggc atatcggcgc
    42541 accacgacaa gaccccttct tcggcaggtg agaaacgtgc gggtgagaac gtgcgagcga
    42601 aacactgcgg actcgaccca tccacggctg tcaggtcgga agcgggccct cgaccccaac
    42661 gccacttccg aacaggtgtt cgagaaacac tctaacctcc cccaccgaca gcctgtcaag
    42721 gccgccgcga gccgccccct gaccacgctc gtcgactcgg cgctggcggc ccacgacgtc
    42781 gtctgggcgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccctgacca
    42901 cgctcgtcga ctcggcgctg gcggcccacg acgtcgtctg ggcggcagcc ggacaccccc
    42961 atgccgtgtt tccgacgacg ttcgccgaat tgatccggat cagcgctggg cgcgcggcca
    43021 ccgtcgcctg acggcgcaaa taagtatccg tactgatgcc gacacttgag tcggtctgcg
    43081 atagtgagcg tgcaacgaag ggcaccgacg tccggccgcg catcaggtct gcagggggtg
    43141 acctgaagtg cggccgggtc gttatgtgcc tccggaccgt tatcagtccg ttgcccgggt
    43201 tcgggaaacc ccggccgctg cacgacgttg aaccctcgga cggagcagtt gactccgtca
    43261 tcagtgggag ggacacacac caccatgggt gcagagattc tgccctgggt cttcacgtcg
    43321 ggctgggcca gcggcatcaa cgcgtatgcc gtcgtgctca tcatggggtt ggccgagaag
    43381 ttctggcagg tccaagggat cccggacgct ctcgggcgca ccgacgtcct catcggtgcc
    43441 ggagtgttgt tcgtcatcga gatggtggca gacaagatcc cctacctcga ctcaacgtgg
    43501 gactcgatcc acaccgtcgt gcgccccgcc gtcggcgcga ccctgggcta cctgctgggc
    43561 aacgagagcg ccgacctcaa cgccgctttc actgcggcga ccggtggcat cctggccctg
    43621 ctgtcccacc tggtgaaggc cgggacccgc gccgccgtca acacctcccc cgagccggcc
    43681 agcaacgtcg tcctctcgac cgccgaagac gtcaccgtgg ccgccgtcgt gaccacggct
    43741 ttcgccaacc cgtggctggc ggcctcgatc gccggcctgc tgctcgtggt cggcatggtg
    43801 acggtgttgt tcctgctcaa acggatccgg cggttccggg cccgctacaa cgcctggggc
    43861 gaccggctgg gttcgagcga gcccaaggcc gacggttcgc tgcgcgacgc accggcccga
    43921 cgcggcgaca ccgcccgtct cccccaaggc cccggagccg actgaccgac tgaccggcta
    43981 aaccgactga ccgactactg cgcggcctcc aggccgaggt tcccccagac ctgcagggtc
    44041 gccgtcgagc ggttcatcgt gatgaagtgg atgcccggcg cgccctcgtc gagcagccct
    44101 tgggccaact cggtggcgat ctccactccg acggcccgca ccgcgaccgg atcgtcggcc
    44161 acccgctcga gacgctcggt cacctcacgc ggcaccggct gaccggacaa ctgcgtcatc
    44221 cgggccagtt ggcggacgtt ggtcaccggc ataagccccg ggatgatcgg caggtcacgg
    44281 ccacgcgcca ccacccggtc acgcaggtcg acatacgcct cgcgtgtgaa gaagaactgg
    44341 gtgatggcga agtcggcgcc cgcgtcggcc ttgcgcacga gcacgtcggc gtcgtggtcc
    44401 aggctcgggc tctcggggtg ccgatcgggg aatgctgcca ctcccacggt gaacgggccg
    44461 agggtgcgca gcagcgacac caactcatca gcgtgatcga gtccgtccgg gtgccgctcc
    44521 caggtctggt ccaacccgcc ctgcgggtcg ccgcgcagcg ccagcacatt gcgcacgccg
    44581 acgttgacgt agtgcccgat gatcgagcgc aactccgcca ccgagtgccc gacacacgtg
    44641 aggtgcgcca tcggcgtcag cgtcgtctcc tgggcgatcc ggtcggtcat ccggatggtc
    44701 cgatcacgcc ccgatccccc ggccccatag gtcaccgaga cgaatgtcgg gcgggtcttc
    44761 tccaggtcgc ggatcgcctc ccagagggcg gcctcccccg cgtcgtcctt cggcgggaag
    44821 aactcgaagc tgatcgaggg ccgaccttgc gcgagcgcgt ccgggatcga gcgcacgagg
    44881 tgcgccgcgc gcgggaggac agccatgaga ccagcctacg agccggtatg ccgccccctc
    44941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agagcgatga ggacgagcgt
    45061 cacatcggtg tcgtcgatcg tcatacctcc ccaggagact ccacccgcga accgggacgg
    45121 cgtgtccccg gtgacggtca ccgctcgcgg aggtgtccct cggacaaggg cgtaagccat
    45181 gtcgaagtca gccccgtcgc cgaggcggag taaccccggg gaccccgcgg gcagggtgaa
    45241 gacgcccccg ccgaggtcga ggtcgcccac gcgacgctgg aggctcacgg cgaccgtgcc
    45301 cgcctccagg cgcacggcat acccaaaatc cccgccctcg tttcgggcgg cacccgcccc
    45361 cacaccgtcg atgtgcagga tccccgggct gagatgacgg acctcccgcc cgccgatggt
    45421 gtaggtgaga ccggcgtacg gggtcatccc cgtgggtggc ggggtgtggc gcaccacgat
    45481 gaccaggcgc ccgttcacga tgatctgcct ggcctcaccc agccgtcgga catccgggac
    45541 gagcgcgacg tccacagagc cggggtccgg gtgatccgct tcggggatca gcacgaggac
    45601 ctcgtgagcc ccagcgctcc ccggcggcgg accccactgc agcacggcgg gagcgaccga
    45661 gccgatgacg ttcaacgctg tggagacctc accgctttcc acccggacac cgaaccgctc
    45721 gtcctgaacg aagatctcct cggacgtcgt cggggcaacg gtggacaccg gcaccgtggc
    45781 cggctgctgc gacaaccgcg tgccagagcc tccggtcaga gcccagctgc caacgaccgc
    45841 gaccgccgca gcagccgcgg ctcccaacac ctgccaacgc cgccgccgac tcagcgtgcg
    45901 ggccccctcg gcgacgatct cgctcacctc caggggcagg tatgccgcgt ccgcccgatc
    45961 cacggcctca cgcagcccta cccgcaggag gtcttcgagg tcggcgtccg tgggtgagtt
    46021 ggagggcgca ttgctggggt ggctcatcgg atctctcctt cctggacgac caactgggct
    46081 cgcagcgctg ccaaagctcg ggatgcgctg gacttcaccg tgccgacgga gattcccagg
    46141 gtgtcggcga cggtgtcttc ggagaggtcg cagtagtaac gcaggaccac gacccgccgc
    46201 tgttgctcgg ggagcccgcg cagcagccgc acgacctcgt cccgatcgtc cgaggtcgcg
    46261 gtgtcgctgc ggtcggccga tcgggtgtcc acttccgcga ccggcacctc gcggcgcgtt
    46321 cgccgccacc gatcggttcg ttcgttggcc aggatccgcc ggcagtaggc catggccgac
    46381 tcactgcgga tctgcggcca ccggacgtag gccttgacca gcgtggcctg gaccaactcg
    46441 tgggccgctt cggacgaacc ggtcagcaac caggcggttc gcagtagccc gggcgaggcg
    46501 acctcgacga actgcgcgaa cgaggcgtcc cgctccctgt cctgcggtgt cacggctccc
    46561 ccttgttgtt gccttcaccc tcctctacgg agcacaagcc cagaaggttg cctcggtcct
    46621 gacccggtgt gagggcaccg taggctgggg cgacctgttc gcgcgcggca tacgacatgc
    46681 cgcctgctcc tggaaggcgc ccggtgacca accccctgga ccgcgagtcg ctgcgctcgc
    46741 gggtgcagtc ggctctcgag gcggagctgg accaccagga gcaggtcctc gccgagctcg
    46801 acgaagacct cacccccctg gtcgcaccgg tccgctcgct gctggcggga ggcaaacgac
    46861 tgcgagccgc cttcgcctat tggggctacc gcgccgcggg tggcgccgac tccgactcgc
    46921 tcgtgcggtt cgccagctcg atggagttgt tccaggcggc cgcgctgctg cacgacgacg
    46981 tcatggacgg cagcgacacc cgcagaggta tgccgtccgc tcaccgcgcc gtcgcggcca
    47041 tccacgccca gcacgcctgg tcgggcaatg ccgatcgctt cggtgaggcg accgccatcc
    47101 tcgccggcga tctgtgtctg cagtggaccg acgaggtcta ctccacgagc gggctgcctg
    47161 ccgagcacct tgccaggggc cgagcgacct tcgaccggat gcgcacccag ctcatgggtg
    47221 gccagttcct cgacatgctc gaatcggtgc ggacttggga gggtcgctca acggatgagc
    47281 gaattgccgc tgcccgcaag gtgatccgat tcaagagtgc gaaatacacc atcgagcaac
    47341 cgttgctcat cggtgctctg gccggaggtg tggatccggc ggccgtgcct gggctctccg
    47401 gttatgggct ggcgctcggt gaggcgttcc agttgcgcga cgacctgctt ggcgtgttcg
    47461 gcgaccccga gcagaccggc aagccggccg gtgacgacgt gcgcgagggc aagcggaccg
    47521 tgctgatcgc ctacacgctg gacgtggccg atgcggggca gcgagccgag ttgacccgat
    47581 ccttcgggcg ggccgacgtg gacgtcgccg ggatcgagag cgtgcgaggc atcattcggg
    47641 cgacgggcgc cgtcgagcgc gtggagtcgg atatcacccg gctcacccac gccgcgaccc
    47701 acgcgctggg tgagcccgcg gcatacctgc agcccgaggc cagcaccgtc ctgggcgagt
    47761 tggtcgcgtt ggcgaccgac cgggtcacct gacctgcacg ggcactattg gcacggcgcg
    47821 gcgcggcgca ctcatggcgt gcgttgccga tgctgacgca atctcaccaa gattgcgtcg
    47881 ggacacgcag cggtgcctcg catcgatcag aaggccaacg cctgagcgcg tcgcctgacc
    47941 tcggtcttga aaccggctga gagggctgcc agtggggtgc tgccgccagg caggctggtg
    48001 tcgacgctgt gcaaccaccg gatgatctcg acgtcgctca tgccaccatc ggccaggacc
    48061 gtgaaggtcc ccttgagctc gggtcgtggc ccatcggggc cgaggaaatc cgtcgggacg
    48121 gcgagggcgc ggttgggtcc cacccggtgg gtgagcaact cgcggtcggc caggaaccgg
    48181 cggacgcggg acagcgccac gccatagcgc tccgcgatgt cgggtaggta ggaccagtca
    48241 ccgacgaggg tctccaaggc gcgcgggtcg tcggaatcgt cgtcggcttc ggacgcgagt
    48301 tgaggttcgg ccacgccccc agcctgccag aagcggccgc gctccgacca ccgtcacggc
    48361 gcacgatcac ctcgggagtc acatccggca ctcccgtcac agtgcgcgcc tgccggggcg
    48421 cacggcatac cccacgtaaa ctccacccgt gacgtctggg ccgggcctgc acgccgacga
    48481 gcgtgtgctc ggcgggcgct accggctgct ctcgcggatc gccgacgggg ggatggccac
    48541 cgtctacctg gccatcgacg aacggctcgg gcggcgggtc gcggtcaagg tgctgcgcgc
    48601 cgacctggca cgcgatgagg ggttcgtcgg acgcttccaa cgcgaggctc ggctggccgc
    48661 gggcctgtcc catcccaaca tcgtcgcggt ccacgacttc cacgagtcgt ccggggtccc
    48721 tggcgaggag ctctacctcg tcatggagta cgtcgacggc cggaccctgc gggaaaagct
    48781 cgtcgatggc ggtgccatgt cggtccggca ggccacacac atcatcacgg ccgttctcgc
    48841 cgccctcgat gaagcgcacc gcgccgacct ggtccatcgc gacgtcaagc ccgagaacgt
    48901 cctggtgcgc gacgacggca ccatcaaggt caccgacttc gggctagcgc gcgcggtcac
    48961 gtccagcacg cgcaccagca cgaccggcat cctcatgggc accgtctcct acctcgcgcc
    49021 cgaacaggtc gatggagatc gcgccgacgc ccgcagcgat gtgtatgccg cgggcctcat
    49081 gctctacgaa ctgctcaccg ggcagcgggc cttccagggc gagaaccccg cccatgtgat
    49141 gtaccaacac gtccacggcg aggtgccgct ggcctccgac cgggtgagca ccgtgccgtt
    49201 ggaactcgac caggtcatcg ccagggcgac cgcacgctcc ccggacgacc ggcccgacac
    49261 cgccggagac ctcatccagg tgctccgcga cgcgatccga caactgtccg acgacgagtt
    49321 cgacgcggct ccagtcttcc tcgccggcat ggcgccaggg acccaaccgg tggctcgcac
    49381 cgctagcccg acaaagtcac tggacgctga cctcgttggc gcagaaggcg atccgagccc
    49441 caccgagcgt ttcgcggcgg tcgaactcgg cgccacccaa cgactgagga cgatcgagct
    49501 cgacgaggac ggcgacggca cgggcgaggt gtcagcaccg gccggcgagc cggtcaagca
    49561 gaagcgacgg cggcggatct ggccctggct gttgaccctc ctgctgctcg tcggcggcgg
    49621 tgtcggtggc tggtggtggt tcgacggcac cggccccggc tcgctgcgga tcgtgccgac
    49681 cctggtccac ctctcgctcg ccgatgccga gaaggcgctc gcgagcgtcg acctgcgctc
    49741 agcaaccacc gaggccttca gcgagaccga gcccaagggc caggtcatct ccgccgaccc
    49801 gaagcccgga gccgaggtgt ccaagcgctc cagcgtcgcc ctcgtggtct ccaaaggcca
    49861 ggagcggtat gccgtcccca ccctcaccgc cgttgctcgc ggcgacgtcg agcggctgct
    49921 caaggaacgc tccctcgccc tcgggacggt caccgaagac ttcagcgaga ccgtggccaa
    49981 ggacgtggtc atcagtcagg acccgcctcc cgacacactg gccaagcgag acaccccggt
    50041 caacgtcgtc atcagcaagg gtcgagcgcc gatccccgtg cccaacgtcg tcaacaagac
    50101 ggctgacgag gcacagaagg cactcgaggc gctcggcctc aaggtggtcc gaggcgaccc
    50161 ggtcaacagc gagacggtcg cgtccggggc cgtcgtctcg cagaccccgg ccacgggcac
    50221 gctgttcaag ggcgacacgg tgacgatcat cccgtccaag ggcccggtgc tcgtccaggt
    50281 gcccgacgtc accctcaaga aggtcaatga cgccaccaag atcctcaagg atctcggcct
    50341 ggaggtgaag gtctcccaaa tcttcggcgg ggtgctcaag actgttcgct tccaggaccc
    50401 ggccgccggc accatggtgc gacgcggcac ggtcatcacg ttgaccgtta tctgacagcg
    50461 cggcatacct catggccatg tcccctgccc gcgcccggac cgtcgcgacg atcctgctgg
    50521 tgacgctcac cgcggtgtgg ggatcgacgt tcttcctcat ccgcgacctc gtgcagacgg
    50581 tgccgccggc cgacttcctc gcggtgcgat tcaccctcgc cgcggtgctc atggtcgtca
    50641 tcttctggcg ccaactgcgc gggctttctc gacgcgactg gctggtctcg gccgcgctgg
    50701 ggtcgctgta tggcttcgcc cagatcctcc aaacgatcgg gctggcccac accgacgcct
    50761 cggtgtcggg gttcgtcacc ggcacctatg tcgtcctcac gcccgtcttc accgcgctgc
    50821 tcctgcgtga gcgcgtcccc gccagcacct ggtatgccgt gctcctcgcc accgcgggcc
    50881 tggccctgtt gtcgctcaac gggttctcgg tcggtctcgg ggaagggctg actcttgggg
    50941 ctgcggcgct ctacgccctg cacatcgtcg ggctcggtcg cctgtcgacc cccgaccgag
    51001 ccaccgggat gtcggccgtg cagatgatcg ccattgcggt gatctgtgtc gtggcggctg
    51061 tccccgacgg catggtctgg ccgagcggcg tcggtcaatg ggggtcggtc gtctatatgg
    51121 cgacggccgc tggggtgctg gcactgtggg cccagacgtg ggggcaggcg cacatgtcgg
    51181 ccacccgggc ggcgatcgtc atgaccctgg agccggtgtt tgcggccggg ttcgccgtcg
    51241 cgttcggcgg cgaggtgctc acctggcgga tgctcgtcgg tggcgcgctc gtgctgaccg
    51301 cgatgtacac cgtcgagctc atcgggcgcc gccctgatgc ccccaaccgc ccggccgaag
    51361 ccctgcacca cgaggtgtga tgacgc
//
LOCUS       DAORPW010000004        51209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_80_length_51209_cov_693.222778, whole genome shotgun sequence.
ACCESSION   DAORPW010000004 DAORPW010000000
VERSION     DAORPW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 51209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51209
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80_length_51209_cov_693.222778"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLB86_00810"
     CDS             <1..569
                     /locus_tag="PLB86_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC63706.1"
                     /translation="GFVNGRKLDKVGQDESDTAELFFENVRVSDANRLGPENGGFIAM
                     MQRLPQERVGAAVSNVAHAFQIFRETLVYTKERKAFGQSIGAFQHNKFKLAEMLTKLE
                     VTQAFVDDCVDAHARHELSSVDASKAKWWSAQVQNDVLDECVQLHGGYGFMNEYRAGR
                     AWRDARVTMIWAGSNEIMKELIGRDLGL"
     gene            604..1362
                     /locus_tag="PLB86_00815"
     CDS             604..1362
                     /locus_tag="PLB86_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC63707.1"
                     /translation="MGSHTGAVAIVTGASRGIGFGIAQRLVTEGARVVITARKPEPLA
                     EAVAALGGSEYAVGLAGNAADEAHQDEAIALARAAFGGLDYLVNNTGINPVYGPILDV
                     DVDVVRKVLDVNVVAAFSWIKKVVAAGLGSPDHPGSVVNIASIAGLRASGTIGWYGVS
                     KAALIHLTMELGHQLGPDVRVNAVAPAVVKTQFAQALYVGREEKVAAAYPLKRLGVPE
                     DIAGAVSFLLSRDAAWMTGQTLVVDGGVTLGGAL"
     gene            1677..3884
                     /locus_tag="PLB86_00820"
     CDS             1677..3884
                     /locus_tag="PLB86_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HRC63708.1"
                     /translation="MGEASSRADDILAALDPEQREVAASPVGPMCVLAGAGTGKTRAI
                     THRIAYGVHSGAYVPQRVLAVTFTARAAGEMRTRLRELGVGGVPARTFHAAALRQLHF
                     FWPQAVGGAAPEVLASKAPVVAESVSRLRMQFDRTAIRDLAAEIEWGKVSLLTPQSYA
                     AGARRAGREAPGIDHTAMARVWESYEERKTERGVIDFEDVLLLMAGILDERMDIARTV
                     RDQYRHFVVDEYQDVNALQQRLLDLWLGGRDELCVVGDPAQTIYTFTGATPEHLLTFR
                     SRYPQARLVRLVRNYRSTPQILGLANLVVRSARPSLRRPGVGGSAAARALPDHVDLQA
                     QGEPGPQPRLTAYPDDPAEAAGVAGAIKALVDGGRPPGEIAVLFRTNAQSEGLESALA
                     ALDVPYLVRGGERFFARQEVRGAIVLLRGAVRGDDGSRPLPELVRDVLTGVGWAATPP
                     TSGGAQRERWESLMALAGLADDLVAASPGARMGDLVRELDERAAAQHAPAVAGVTLAS
                     LHAAKGLEWDTVFIVGCSDGFLPIVMAEGEVAIEEERRLFYVGLTRARRDLQLSWSGA
                     RTPGARATRRPSRFLDPAASILGEGARSAPRRSGVVGGSGSSPSAPRQPRLPSVCRGC
                     GTELTTAATRKVGRCEDCPPTYDEATFEALRVWRGEVAKTMKVPAYVVFTDATLTALA
                     ETAPTDLRGVAGVSGVGAMKLDRYGGAVLDVLAGGDPHMAAEKTTAAMGSDNS"
     gene            4028..4276
                     /locus_tag="PLB86_00825"
     CDS             4028..4276
                     /locus_tag="PLB86_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63709.1"
                     /translation="MEINTMITRTTARLGDSGIGSDHLLLPTVPAVQGGTLPTPRRVV
                     IAGPAAVASNGEGVAFAAAYAASPKTDVHRSATGSPPV"
     gene            4520..4807
                     /locus_tag="PLB86_00830"
     CDS             4520..4807
                     /locus_tag="PLB86_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRC63710.1"
                     /translation="MQLSTLIDTQTAAMSVGVGIPCRDYDAELWFAERPADVEFAKSL
                     CGTCPARTACLAGALERGEPWGVWGGQLLVQGTIVARKRPRGRPRKNDVAA"
     gene            5261..5839
                     /locus_tag="PLB86_00835"
     CDS             5261..5839
                     /locus_tag="PLB86_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HRC63711.1"
                     /translation="MQANLTIAQDPEADALISRDPFALLIAMLLDQQFPMERAFAGPA
                     KLLARFGTLDPVRVAEADPEAFADLAATPPAIHRYGRAMAGRIQQVAAHVVEHYDGDA
                     EAIWTTAKGPDELIRRLVALPGFGEQKARIFAALLGKQLGVRPRGWREAIGPYAAPGT
                     FRSVADVVDAESLAKVRAFKQAAKAAAKASAQ"
     gene            5836..7290
                     /locus_tag="PLB86_00840"
     CDS             5836..7290
                     /locus_tag="PLB86_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63712.1"
                     /translation="MSEAPALGVVFPVEGDGRSTSATGRAVIADALEVVDPVGARAAR
                     DEANWRTGYPLHFRRLVEAGLGTAADAVGIADAGLTSVHSRMRWSRPEGTEVALGEAL
                     SGDLRPLETDRLAGSGEPEAAFSLPYRGARLSGDAAVAQVHDWVSRGIIEPSCGDAVE
                     QVIAHPEWLDLSDFTFVVLGAAAEMGPLRALLRWGADVVAVDLARPDLWRGLLSDTRR
                     FGGTLRIPVAAGSAPVEERAGVDLVTEWPSLSAWLVEQPGTLVLGNYVYADGADNVRV
                     SVAVDALSMSLRAQRPDTALAFLATPTDVFAVPPADVERATAAYANARVSKALRGPLR
                     GLSGGRLLSRNYAPQANPGIADAIVPQQGPNYLLAKRIQRWRATTARAEGATVSMTVA
                     PPTRTRSVVKNRALAAAYAGAHRFGIEVFDPATANTLMAAVLVHDLRTGTKTLGHPWL
                     DEAHQAAHGGLWTAAYSARSALGLAAVLGLGSRS"
     gene            7287..7475
                     /locus_tag="PLB86_00845"
     CDS             7287..7475
                     /locus_tag="PLB86_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63713.1"
                     /translation="MTVAEDRIVCISCGSMPSPDDEGTARVEWVRGVENGRPVWTCPE
                     CSRRNLRSMEGKLDSAWW"
     gene            complement(7550..8194)
                     /locus_tag="PLB86_00850"
     CDS             complement(7550..8194)
                     /locus_tag="PLB86_00850"
                     /inference="COORDINATES: protein motif:HMM:NF025791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-4 family immunity protein"
                     /protein_id="HRC63714.1"
                     /translation="MGMPPTSGTADRAGPDRIRLTPDEAAALDLPPTLHDWLTTTGLP
                     VLDGETLGVTFQHPFRVTPTPSAASTKRWPRLLDIGYEQWEPEHLRVGIDLDTLAVVG
                     YGPEPADNAFINSSVQTFITFLEHYRPVSDALKPAESSFRIIGPEEAAEMIRQLHEGT
                     LKPRPKPKTEPKPRINRKKAIDELRDTFKAADPDALKRNTYWPRIISQLRDGLI"
     gene            complement(8335..9663)
                     /locus_tag="PLB86_00855"
     CDS             complement(8335..9663)
                     /locus_tag="PLB86_00855"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRC63715.1"
                     /translation="MTDLPRAAVTRAAKLATLPLGYAGRAALGLGKRVGGKPAEAVAA
                     EMQARAATQLFTVLGELKGGAMKFGQALSIFEAAWPEEAAAPYRAMLTKLQDSAPAMP
                     PARVKEVMAAEFGPRWRRRFLDFDETAVAAASIGQVHRATWRDGRDVAVKIQYPGAGK
                     ALLSDLSNVSKVARLATSWIPGIDIGPILEELRGRMSEELDYRLEASAQKAFAKGFEG
                     DPNVAVPGVIDGTERVLVTEWMDGIPLSSIIADGTQEQRDLAGTHYIEFLLDAPGRCG
                     LLHADPHPGNFRLLEDGRLGVLDFGAVKHLPGGLPDDMGRLLTLALDDDAGATLAGLR
                     EIGFVRDGIDVDAQRLLDYLQPFIDPLRTDTFHFSRDWIRDVFAHINDPRKPNYAVGM
                     RLNLPAEYLLIHRVWLGGIGVLCQLGATVPGRAKVAAHIPGADLPPVDEE"
     gene            complement(9668..10720)
                     /locus_tag="PLB86_00860"
     CDS             complement(9668..10720)
                     /locus_tag="PLB86_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63716.1"
                     /translation="MSQRGPRPDRSARASQPRLHPRRPPLRRGVDEVQLGVGPQALVL
                     RGLSEGQVAVLERLDGSRSLAQLHDVAADFGDPPHVVDELLTLLGRLGAMADPRSERV
                     DLLAARLGSEPRVLVGGPDALTDRLARALRQAGLEGVRVGPLALDQAELALRDREHTV
                     AGRQRPPDLVLLSSFDGLDPAEAAPWARHGIAHLAFTTEGDRVVVGPLVVPPPRGDTT
                     CIQCLELHRCDRDSGRASVLAQTSRPPAALADPRQWTEGLTALLGPGGPVRPTPMEPA
                     LLSAAAAVAALVVAAYLGGDCLPDGVTVELTSPWPRLDHRKWPRHPQCPHHRRAVAPT
                     PTIGAVVGSAAAAGRQ"
     gene            complement(10884..11048)
                     /locus_tag="PLB86_00865"
     CDS             complement(10884..11048)
                     /locus_tag="PLB86_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HRC63717.1"
                     /translation="MANDETYAGEFYCVKCKEKRTAEGKVVVSENGRRMAKAKCPVCG
                     TNLNRILGKA"
     gene            11279..11896
                     /locus_tag="PLB86_00870"
     CDS             11279..11896
                     /locus_tag="PLB86_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRC63718.1"
                     /translation="MDLDVALPFEVDAGHSQSPVDTPAVPTRGRRATSQEQPLVEVRR
                     STRRRRTVSAYRDGDRVVVLVPARFTRAEETRWVQTMTERLAASEAKRRPSDTDLAAR
                     AEALSERFLGGRARPASIRWVANQRGRWGSCTPSERTIRISSRVQGMPGYVLDYVILH
                     ELGHLLVAGHGPDFWAVLSSYPRLERARGYLDGVAAASGLALSDD"
     gene            11904..12665
                     /locus_tag="PLB86_00875"
     CDS             11904..12665
                     /locus_tag="PLB86_00875"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HRC63719.1"
                     /translation="MARTAASSPARPGRRRRSPGWVPTQHGAWAMLVSPLLLGALASG
                     PGWIHLLLAAFWFAGYFAFNATSLWLKSGRKVRYLRPVQVYAALAAALGLATALADPG
                     LLRWAPLFVLPLGVGVWAAAHRRERDLLAGVTTVIGSALMTVVAYDAGGGPDLHRAAV
                     LGAGQFLYFAGTVFYVKSMIRERDNPAYLRLSVGFHVVAALALAPWAPWVAALFVVLA
                     LRAWWVPRLGWTPKQIGIAEIAGTTAVALVSLFTV"
     gene            12712..14562
                     /gene="ilvD"
                     /locus_tag="PLB86_00880"
     CDS             12712..14562
                     /gene="ilvD"
                     /locus_tag="PLB86_00880"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRC63720.1"
                     /translation="MPQLRSRTTTHGRNMAGARALWRATGLTDDDFGKPMVAIANSYT
                     QFVPGHVHLKDLGDLVAGAVREAGGVAREFNTIAVDDGIAMGHHGMLYSLPSREIIAD
                     SVEYMVNAHAADALVCISNCDKITPGMLLAALRLNIPTVFVSGGPMEAGTAVIGEDGT
                     ATTRLDLIDAMSKAVDDHVSDADIATIERSACPTCGSCSGMFTANSMNCLLEVVGLAL
                     PGNGSTLATAAARKGLFESAGRLVVELAKRWYAVDGTEDASVLPRSIVTREALSNAMR
                     VDIAMGGSTNTILHLLAAAQEADIAFDQHDIDALSRVTPCLCKVAPNTNRYYMEDVHR
                     AGGIVAIMGELNRAGMLDDSVHAVHAPSLQAWLDEWDIRGGKTSDAAYELFHAAPGGV
                     RTTEAFSSTNRWATLDTDAREGCIRSVEHAYTVDGGLAVLTGNLAPDGCIVKTAGVPE
                     HQWTFRGPAKVAESQDAAVEMILAGNVVAGDVVVVRYEGPKGGPGMQEMLYPTSYLKG
                     VGLGPKCALITDGRFSGGSSGLSVGHVSPEAASRGAIGLVRDGDEIEFSIPERRVTLH
                     VSDDELDRRRVEQDAAGWQPVAREREVSAALRLFALFAQSADKGAARKVP"
     gene            14676..15482
                     /locus_tag="PLB86_00885"
     CDS             14676..15482
                     /locus_tag="PLB86_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HRC63721.1"
                     /translation="MLRRLALVRQPSPRLADGIVTHIDRESVDPVLARDQWEAYVGAL
                     RANGWDIVQAPAAPDCPDGVFIEDQVLVYGDLALLCRSGAPERRAEQDGLAGLLTDVG
                     YRVARIEAPGLLDGGDVLKHDGRVWVGDAGSTGRSNRAGVEQVADLLAPLGARVEAVP
                     LSGVLHLKSAVTALPDGRILAGPSLSAVTAEWPGWGTSSRGASRLLAVPEESGAHVVI
                     LDADTVLLAASAPQTAEVLTGQGLRVVTVDISEFEKLEGCVTCLSVRLRR"
     assembly_gap    15566..15665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15896..17746
                     /locus_tag="PLB86_00890"
     CDS             15896..17746
                     /locus_tag="PLB86_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HRC63722.1"
                     /translation="MTDSLTSTKASPADLAAQNRQRPSAPAAPAVPAVPEPCTGAQSL
                     VKSLEAIGVDTVFGLPGGAILPAYDPLMDSTKVRHILVRHEQGAGHAAEGYAVASGRI
                     GVCMATSGPGATNLVTPIADAYMDSVPMVAITGQVNSAAIGTDAFQEADIRGITMPIT
                     KHNYLVTNPDEIPRAIAEAFHIAGTGRPGPVLVDISKDALTGSTTFSWPPVIDLPGYR
                     PVARPHGKQIREAARYIAAARQPLFYIGGGVIRARASEVLRTLVDLTQIPVVTTLMAR
                     GAVPDSHPLHLGMPGMHGSVAAVTALQKTDLIIALGTRFDDRVTGQLSTFAPNAKVIH
                     ADIDPAEISKNRTADVPIVGDCKDVITDLIEAITAEQAAHGAAEYEAWQATVAEWKET
                     YPVGYTTTPDGLSPQYVIERLGALSGPEATYVAGVGQHQMWAAQFVQYSRPNSWINSG
                     GLGTMGFAVPAAMGAKVAEPHRTVWAIDGDGCFQMTNQELATCVINDIPIKVAVINNS
                     SLGMVRQWQTLFYNERYSNTDLHTSHNQQRIPDFVKLGDAYGCASWRVERPEEVDEAI
                     IKALEINDRPVVIDFVVHRDAMVWPMVPAGVSNDKILIARGGAPLWEREA"
     gene            17807..18319
                     /gene="ilvN"
                     /locus_tag="PLB86_00895"
     CDS             17807..18319
                     /gene="ilvN"
                     /locus_tag="PLB86_00895"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRC63723.1"
                     /translation="MPKNTLSVLVENKPGVLARIAALFSRRGFNIDSLAVGPTEHPDV
                     SRMTVVVDVDELPLEQVTKQLNKLVEVLKVVELEPQSSVQRQIVLVKVRADASNRSHI
                     VETAELFRAKVVDVASDSLTIEATGKVEKIQALLGVLEPFGIRELVQSGVVAVGRGPR
                     SITDRALRSA"
     gene            18444..19472
                     /gene="ilvC"
                     /locus_tag="PLB86_00900"
     CDS             18444..19472
                     /gene="ilvC"
                     /locus_tag="PLB86_00900"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRC63724.1"
                     /translation="MADMIYDDDADLSIIQGMKVAVIGYGSQGHAHALNLRDSGVDVR
                     VGLAEGSKSRAKAEAEGLPVKSVAEATKEADLVVILTPDQVQRHVYSDEIKPNLKEGA
                     GLLFGHGFNIRFGYITPEPGSDVVMVAPKGPGHLVRREFNDGRGVPVLVCVEQDASGR
                     AWDIAKSYAKAIGGLRAGGIKTTFTEECETDLFGEQSVLCGGASQLVMYGFETLVEAG
                     YQPEVAYFECLHELKLIVDLMVEGGIAKQRWSVSDTAEYGDYVSGPRVIDPSVKDNMR
                     AVLTDIQSGAFARRFIADQDAGAPEFKALRAKGAQHPIEATGRDLRKLMSWIKETDSD
                     YTEGSAAR"
     gene            19600..20103
                     /locus_tag="PLB86_00905"
     CDS             19600..20103
                     /locus_tag="PLB86_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018134254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRC63725.1"
                     /translation="MFDSLPFPLVYAVFFLGAMIRANATYWLGRGARKGGETTRAARR
                     LHSPAIRRAETVISRFGAPAVALSFLTVGVQTALNFAAGVLRMPLWRYEVGVVIGALM
                     WAGIYSTIGFAVFEAWVSQSPWLWLGAAIGLIALVVVATRLTNRRLESTPPYASFPDV
                     ADSGGRD"
     gene            20179..20721
                     /locus_tag="PLB86_00910"
     CDS             20179..20721
                     /locus_tag="PLB86_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC63726.1"
                     /translation="MGSQTPASDVPGAEFRARAVRTTQERRPDVDPRAIALSLTLARI
                     AATHQHQSERLVHRERGWSYSGFRVLYMIWLTEPVEARDLARFAGVSRQTTSTVLSTL
                     EAARLVVRRQTSKTDRRLVSVRLTPRGRAAVDEAIGAQNALESDWFSVLDSAEQAQLQ
                     DMLERVLTRIDRPAETPAAD"
     gene            20907..22520
                     /locus_tag="PLB86_00915"
     CDS             20907..22520
                     /locus_tag="PLB86_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRC63727.1"
                     /translation="MQQLTALDVMFSSLDTETTNGVLGGLVLHEPAADGRPAADAAFM
                     RQRFAERLPFLPPLHREVVKVPIGIDHDYLGQPDRIDLLEHIRSISLPAPGTQDQLAA
                     EVSRIMSTSLPTGRPLWDYTVIDGLADGSVAHLLRIHHLVIDGGSMPTLWDALSDEPA
                     APLEAWAESTRPQPRFGKGEMLARELGGVVAKPVQFAAFQAKFLAWVAGQTKEKGPLA
                     PLALPFRVSLPGGLAQPAQALFNSALSKRGIAEIQPYIPTLRTPTTPFNNRVTAQRTF
                     VFADLPLAQFRDTGKALGGTINDVVLGVCAGALRRYLVENGIPTDDPLIVCVPVSIRT
                     GAETTKWANYVHMIFAPLPTHLDDPLARVRAAGAAVKAAKGNFDALPVGLIREASRFI
                     PSAMFNVMTRAMVKMPDKVSKGPWNVVVSNVRGPSTAAYMDGVKIKGYWPASFLSVGG
                     GINITLQSYTDRICFGFMGAPEQVGDLRPLIGYMTDALAETVAAAGDSSAKAAPAEAK
                     AARKRTPHRAGPGSARLAAVAPAAVGDAT"
     gene            22534..23073
                     /locus_tag="PLB86_00920"
     CDS             22534..23073
                     /locus_tag="PLB86_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRC63728.1"
                     /translation="MLGEPHDRPRPAASDFVDTVADLTQRRAEGADPETTALVVALLR
                     AGGRHRGLFERRAHDQGGRHLRAFSVLYLVWLYGRLRARDLARLLGMSRQTTSAVLAS
                     LESGGLVCRERGEGRDRRLVEVRLTDDGRRTVEREFQAQHDLDGQWLAVLSPAEQRTL
                     RHLLERLATQHPGDNTLVG"
     gene            23111..24643
                     /locus_tag="PLB86_00925"
     CDS             23111..24643
                     /locus_tag="PLB86_00925"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRC63729.1"
                     /translation="MPDITVGAWFAARAARSPQRRALTFEGTSWTFAELADRIERLAA
                     VLRAGGVKHGDRVSFVGFNQPAALETWFACSRLGAIYVPLNFRLAGPELAFIVGDAGV
                     HTLIAGADQQPGIDAIRAELGVRRYLVTEADGIPEGWESYEQAVTAAHPLPTPDPVRP
                     DELSIIMYTSGTTGTPKGVMLSHENLWWSDIALMSWIDIHADDVTLAFSPLFHIAGLN
                     FMIGITWMRGGEVILHRSFDAQRTLEDIARYRVNTLFGVPAMFIAISQLPGFHAADLS
                     TVRMAVCGGAPAPEPLLRTFADRGIGVLQGYGLTETSPAAIFLVAEYALSKLGAAGQP
                     PLYIETKLVGSGGVTVSEPGVRGEVCMRGPNVTRGYWNRPEATAAAFDEAGWFHTGDV
                     GQFDEDGIISIVDRVKDMVISGGENVYPAEVESVLFGHPSVAEVAVIGLPDARWGEAV
                     TAVVAAKPGQSVTLEDLREYAASHLARFKLPQRLHLVEVLPRNPTGKVLKYQLREDLG
                     AK"
     gene            24799..26382
                     /gene="serA"
                     /locus_tag="PLB86_00930"
     CDS             24799..26382
                     /gene="serA"
                     /locus_tag="PLB86_00930"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRC63730.1"
                     /translation="MTKPVVLIAEELSPATLDALGPDFEIRSCDGADRAVLLPALADV
                     DAVLIRSATKMDAEAISAAKRLKVIARAGVGLDNVDVPAATQAGVMVVNAPTSNITSA
                     AELAVGLLLASARNIAPANQALKAGAWKRSKYGGVELLDKTVGIVGLGRIGALVAERL
                     KGFGMQVLAYDPYTTPAKAGQLGARLVELDELLAQADFITVHLPKTKETLGLIGVDAL
                     SKVKPTVHIVNAARGGIVDEVALADAIREGRVAGAGIDVFVKEPCTDSPLFEFEQVVV
                     TPHLGASTEEAQEKAGIAVAKSVRLALGGEIVPDAVNVSGGVIVEEVRPGIALVEKLG
                     RVFTAVAGAVPLHLDLDVCGEITEHDVSIWKLAALKGLFTDVVEDQVSYVNAPVIAEA
                     RGVGVTLTTDSASGDFRNVTTLRGTLKDGTEVSVAGTLTGPRMIEKLVGINGFDLEVP
                     LSDHLAAFTYADRPGIIGQVGGILGDSAINVGGMQVARSEKRDTALMVLSVDSELPAE
                     VAARIAEGIGAVHFAVVNL"
     gene            26468..27544
                     /locus_tag="PLB86_00935"
     CDS             26468..27544
                     /locus_tag="PLB86_00935"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRC63731.1"
                     /translation="MVDTSVTTPEPTSLNLAVIGGDGIGPEVVGEGLKVLAAVTGGAV
                     SISTTDYDLGARRWHATGETLPDSVLAELRGHDAILLGAIGDPTVPSGVLERGVLLPI
                     RFALDHFVNLRPAKLYPGVASPLDIARVAPDGIDFVVVREGTEGPYVGNGGLLRAGTP
                     HEVATEVSVNTRFGVERVVRDAFARAQGRPRKHLTLVHKHNVLTFAGHLWRRTVEDVA
                     KEFPEVQTAYQHVDAATIFLATDPGRFDVIVTDNLFGDIITDIAAAIAGGIGLAASGN
                     INPTRSTPSMFEPVHGSAPDIAGQGKADPTAAILSVAMMLDHVGLADAAARVEAAVAA
                     DLVERGNQVRSTVAIGDAIAARLA"
     gene            complement(27590..28756)
                     /locus_tag="PLB86_00940"
     CDS             complement(27590..28756)
                     /locus_tag="PLB86_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRC63732.1"
                     /translation="MNRVVRALRGNHGRYAASAPLAARPLVAGGALGTAGVIAVLLFS
                     ASSHADVSSSAVGSTVAAGRPSATSSTTAGGESGLTKRPESEVPHFNAVNAMTAAAAN
                     AAAAQAAALAELDAAPDAGSVPTAVVDAAQVLASGFVKPVDGVASSPFGIRFHPILHV
                     WKLHSGTDLATSCGTPVKAVKAGQVTLTGGFTGYGNRVVIDHGSGLVTTYNHLRSYAA
                     AAGLKVQQGQIIGYVGSTGMSTGCHLHFEVMVGGAFVDPAPYLDLAPSPTVVIPAAVP
                     ARSDVILAPAPTLTTTPPVTPTLTPTETAVPTETVVPTATASPTETATASPTDVPTST
                     VGPSPTSDPTSTATDPPSLTSDPTSPTSEPSAPTATPTSDPVQSSPTATDPASP"
     gene            28911..30029
                     /locus_tag="PLB86_00945"
     CDS             28911..30029
                     /locus_tag="PLB86_00945"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRC63733.1"
                     /translation="MTGQDRPAELHFELTPNDHPRSDEERAALLANPGFGKVFTDHMA
                     LATWTTEAGWHDAQVRAYGPLSLMPAAAVLHYAQEVFEGLKAYRHADGSIWSFRPEAN
                     AQRLQRSCRRLALPELAVDDFVGSLKALVEVDKAWVPAVGTGETSLYLRPFMFASEAF
                     LGVRPAAAVTYAVIASPAGAYFSGGLKPVTLWISQDYARAGAGGTGAAKCGGNYASSL
                     AGQLEGIEHGCDQAVFLDSGTHTYIEELGGMNLFFVTSDGKLVTPELTGTILEGITRS
                     SILELAKELGLEPEERRIPIEEWKDAARSKKITEIFACGTAAVVTPVGELRWEGGSVD
                     HRGDGPAIGEVTMTIRERLLDIQYGRVEDTNGWLTRLA"
     gene            30026..30874
                     /locus_tag="PLB86_00950"
     CDS             30026..30874
                     /locus_tag="PLB86_00950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRC63734.1"
                     /translation="MSRLRGSGMPPAPVSEDAVRPRLVASDLDGTLLRPDGSVSDRTR
                     RAVWGLADQGIAFVIVTARPPRWLHELADLVGPHGAAVCANGAFVYDVAARTVREVHG
                     IPDGLVTDLVGELRAAVPGVRFGAERASGAMVEPGYPGADLDRGAEAVRVAPIQDRGG
                     EPVGKLLAVAPHWDEADFLDRVASVVGNRAHLAYSGAHGLAEITGPGVTKAAGLARWA
                     GDLGVGSTDVWAFGDMPNDLPMLRWAGRSYAVANAHPDVLAAASHRCPANSQDGVAWA
                     LESLVR"
     gene            31043..32692
                     /locus_tag="PLB86_00955"
     CDS             31043..32692
                     /locus_tag="PLB86_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRC63735.1"
                     /translation="MADLRLDDDERKLAELGYKQELSRSWSGFSNFAISFSIISILAG
                     CFTTFGQAWNNGGPVAISIGWPLISIFILVIGLCMSELVSAYPTSGGIYWWAAKLGGA
                     KAGYYTGWLNLIGLIAVDASVAYGCATFFDLTFSTFSESWAEGYSLARVFWMFLVILI
                     LVAVVNIFSGHLMAVMNNVSVWWHVFGALAVILILVFLPKSHMSVADVFTMRVNNSGG
                     LAGGSTDGLGYWFYVLPLGFLLTQYTITGFDASAHLSEETQGASHGAARGIWQSIFYS
                     AIGGWILLLAFLFAVQDAEEVTAGGGGVAVIFNQALTSGWAGIVLLIASIGQFFCTTA
                     CMTSTSRMLFAFSRDGAVPGASLWAKLNSSKVPANAVLASTVVAALITIPALKEVNIG
                     TEDAPIIVPTAFYAVVSVAVIGLYLAFLIPIYLRWRAGDKFKQGPWNLGTKWKWMAPI
                     ACLEIVVISVYFILPFTPAAWLGNEAFDWKFVNYAPILTGGTLILLWIGWHASAKKWF
                     TGPKTTIDLPAGVSSAEEIAMEHHGKSAHPAQDADGKRPSS"
     gene            32758..33621
                     /locus_tag="PLB86_00960"
     CDS             32758..33621
                     /locus_tag="PLB86_00960"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HRC63736.1"
                     /translation="MTELPGGLTNHNYRVRTATADVVVRVSPPGTDLLAVDREVEHHN
                     SVAAAAAGVGAPVIDYLPGRGVLVVGFLTARTYSEDDVAANLPRIAAALRRLHAGPPF
                     VGRLDMFALQRRYLALAHERGMCMPSGYDSLAPVVARAEAALAAYPEPLVPCHNDLLA
                     ANFLDDGQDVRIIDYEYSGLNEPAFELGNIAQESGLSDAALVGLVEAYYPHADARLLA
                     RARTWRAISGYGWTLWGVIQHHVSGIDADFWGWATHKYDTAATLLRDSAFVDTLSVAA
                     AAPAPTPRAPA"
     gene            33618..>34909
                     /locus_tag="PLB86_00965"
     CDS             33618..>34909
                     /locus_tag="PLB86_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC63737.1"
                     /translation="MSPTDTLPSRARVVIIGGGVIGASVAYHLTALGWTDVVLLEQGT
                     LSCGTTWHAAGLVGQLRASEAGTRLVQYSCELYDRIEAETGLSAGYRRCGGLTVARTP
                     ERMVTLRRTAASAAAYGLDCDLLTPDQAGERYPLIRTDDLVGAIWLPADGRANPTDLT
                     QALAKGAKQRGATILERTRVLDIHTGPATRGVRVTGVSTDRGDIEAEIVVNCAGQWAK
                     AVGELVGATVPLHSAEHFYVVTEQIEGVHRDLPILRDPDGYTYVKEEVGGLLVGGFEP
                     VAKPWVAPDQIPYPFEFQLLDEDWEHFEILMSSAVHRLPVLAETGVRKFYNGPESFTP
                     DNQFLLGEVPGVAGFFVGAGFNSVGIASAGGAGRALAEWIVEGEPTSDLTAVDIRRFA
                     PFNANNDWLRDRVGEVLGLHYSIPWPNRELETARPFRRS"
     assembly_gap    34910..35009
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <35010..35933
                     /locus_tag="PLB86_00970"
     CDS             <35010..35933
                     /locus_tag="PLB86_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HRC63738.1"
                     /translation="GMLNSAGGYEADVTVTRVAPTQFLVVSGAASAVRDVDWIRRKAP
                     DGHRTEVVDVTAMYAVFGVMGPASRELLTRLSGADLTDAAFPFATSRELRLGYATARA
                     TRITYVGELGWELYVPVEFAAGVYDELFNAGHLLGAAPAGYYTIDAMRLEKGYRAFGR
                     ELVPDATPIEAGLSFTCKLASGIDFLGRSAVERVKTAGPSRRIVSIVVGDPTAEPLAG
                     DPAAYLWGGELLLRDGEPVGQVTSAGWGHALGAPVGLAWAGSRQTGPVTPEWLRTGGY
                     EVDVAGRRVPVTVSLRPPFDPDGVRLGRGPA"
     gene            35930..36667
                     /locus_tag="PLB86_00975"
     CDS             35930..36667
                     /locus_tag="PLB86_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRC63739.1"
                     /translation="MIIPLHNDLSEHVLRPSSGNAFELTVEQLATAIRLGAFRDGDLL
                     PPERELAERLAVSRSTLREAIAALRDSGLVTTRRGRGGGTMVTYAGEAPGSVPLPQPQ
                     RTATAIADVLDFRRVVEPGAAALAATRTLAADQRAWLLACAAQVREAPDSATHRLNDS
                     RLHLALATLSSSPMLVEAVTRAQAALHELLRGIPVISRNIAHSHDQHDAIVAAVLAGD
                     PETARAVMEEHCDATSLLLRALLRPRP"
     gene            36696..38060
                     /locus_tag="PLB86_00980"
     CDS             36696..38060
                     /locus_tag="PLB86_00980"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRC63740.1"
                     /translation="MTRPELTLDQLRAEIADGTIDTVVVAFTDMQGRLQGKRIHGQWF
                     VDTVLGHGTEGCNYLLAVDVEMNTVDGYAISSWETGYGDMMFDLDLTTLRRTPGQNYS
                     ATVQCDLAWLDGRGLVRQSPRSVLRAQVDAAAALGFTALAGTELEFIVFEQSYAAAWD
                     ARYVGLTGVNRYNVDYSIQGGTVVEPLLREIRNEMYAAGLKVESAKGECNLGQHEIAF
                     RYEDVLRTCDNHVIYKNAAKEIAAKHGQSLTFMAKYDEREGNSCHIHLSLRGTDGSTV
                     FADDGREGGHSDVFDHFLAGVQATLRELTYFYAPNINSYKRFQPGSFAPTAIAWGRDN
                     RTCALRVVGHGSGLRVENRVPGGDVNPYLAVAAMLAGGLHGIREGLALEPRFDGNAYA
                     SDKARVPTTLVEARELLLGSAVARAAFGDEVVEHYAHAAQVEVDAFNRAVTDWELIRG
                     FERL"
     gene            38092..39468
                     /locus_tag="PLB86_00985"
     CDS             38092..39468
                     /locus_tag="PLB86_00985"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRC63741.1"
                     /translation="MSSTHEVINPSTEQVVATVPSLSVEETDAAIARAEAAFPAWRAV
                     SPADRGRLLRRFSEVVDAHIDELAQLEVTGSGHTIGNARWEAGNVRDVLAYYAAAPER
                     LFGKQIPVAGGLDVTFKEPLGVVSVIVPWNFPMPIAGWGFAPALAAGNVVVLKPAELT
                     PLTAIRLGELALEAGLPEGVFTVLPGKGSIVGERFVTHPAVRKVCFTGSTEVGQRIMR
                     GAAEQIKRVTLELGGKSANIVFADSDLEKAAASAPMSVFDNSGQDCCARSRILVQASV
                     YDRFMELFETAVKGVVVTDPADEAAQMGPLISADHRAHVRGFVEGSADSVDVAFRGTA
                     PTGAGWWYPPTVVIPRSTQDTVWQEEIFGPVVAVLPFDDEADAIAKANDTAYGLSGSI
                     WTRDVGRAIRVARGVEAGNLSVNSHSSVRYATPFGGFKQSGLGRELGPDALDAFTEVK
                     NVFFATDD"
     gene            39521..40288
                     /locus_tag="PLB86_00990"
     CDS             39521..40288
                     /locus_tag="PLB86_00990"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HRC63742.1"
                     /translation="MSGRLDGKVAVITGGCSGIGLATVRRFVAEGAKVVVGDLNDEAG
                     AALADELGILYVRCDVTSKEAVDAMFAAAKEHFGSVDIAFNNAGISPPDDDSILDTDL
                     DAWRRVQEVNLTSVYLCCKAALPYMIEQGKGSIINTASFVAVMGAATSQISYSASKGG
                     VLTMTRELGVQFARQGIRVNALCPGPVNTPLLQELFAKDAERAARRLVHIPMGRFGEP
                     EEMASAVLFLASDDSSFMTASTFLVDGGISAAYVTPL"
     gene            40313..>40473
                     /locus_tag="PLB86_00995"
     CDS             40313..>40473
                     /locus_tag="PLB86_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRC63743.1"
                     /translation="MGQPRRPVIGITCYVESIDRPPWVSQPSAVLPRMYLEHVARAGG
                     IPVILPPGD"
     assembly_gap    40474..40573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <40574..40831
                     /locus_tag="PLB86_01000"
     CDS             <40574..40831
                     /locus_tag="PLB86_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493169.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRC63744.1"
                     /translation="GVLGAETVEVPTYHHQGVATHPGYAATAWTADGVVEAFEDPQAR
                     FRLAVQWHPEAAEDSSLFRSLVAAATTWSASRDTLSGSGPE"
     gene            41022..42611
                     /gene="cimA"
                     /locus_tag="PLB86_01005"
     CDS             41022..42611
                     /gene="cimA"
                     /locus_tag="PLB86_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRC63745.1"
                     /translation="MSTASPELHVYDTTLRDGAQQEGLNLSVVDKLAIARQLDELGVG
                     YIEGGWPGANPKDTEFFARAQQELDLRNATLAAFGATRRAGSTAAADPLVRALLDSGA
                     SVVTLVAKSHARHVELALRTTLAENLEMIRDTVTFLRGEGRRVFLDAEHFFDGYRLDR
                     AYALECVRTAAEAGAEVAALCDTNGGMLPAQIADVVRDVLDSTGARVGIHCHNDTGCA
                     VANSMAAVAAGATHVQGTVNGYGERTGNANILTLVSNLQIKQGMPLLEPQRLREATRI
                     SHAISEVTNVPPYSRQPYVGSSAFAHKAGLHASALKVDPDLYQHTDPALVGNAMRTLV
                     SDMAGRASIELKAKEMGVDLTDQSELVAAVLAEVKELELRGWTFDAADASFELLLRKH
                     LGQAVTYFDVESWRVITDAREAGEAVSEATVKLTAGGSRQVATGEGNGPVNALDHALR
                     VALMGVYPELARLELVDFRVRILDASHGTDAITRVLIETSDGTTTWQTVGVAPNIVEA
                     SWRALVDGITYGLSRQGVSAR"
     gene            42608..43183
                     /locus_tag="PLB86_01010"
     CDS             42608..43183
                     /locus_tag="PLB86_01010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC63746.1"
                     /translation="MSIDHAAPPQTAHATRHTAPAAGVGLVLRPARADEAPLLSELAL
                     RSKGWWGHSASFLRACEAELTLSPEQAAKAVLVEVDGVVAGFHLLAPSSVGSDVGELD
                     MLFVDPPFIGSGIGRILFEDARRSAAGRGWTRVQIEADPQARPFYERLGAVQVGERLS
                     RSVPGRSLPLFELTLRGGGSSRPIGRATYWR"
     gene            complement(43260..44180)
                     /locus_tag="PLB86_01015"
     CDS             complement(43260..44180)
                     /locus_tag="PLB86_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRC63747.1"
                     /translation="MLMGIDIGGTKTAVVALGPDGSVVAERRAPSGHGPDGVVAVALA
                     VAHECADAVGGWTQIASVGACMPGLVDPRSGLVTHALNLGVDALPLGPALSDALSRPV
                     VIDNDVKAAGWGAFQLHGGDPDTAFGYLNIGTGLAAALVINGQIVRGPGGIAGEIGHL
                     PLAAPVPCRCGQTGCLETSASGSGLTRMWPPARHLADPFAAAAAGDAQAAEAVRHLCG
                     GIAMAIQALVLVGGVEHIVIGGGLTGLGEPLSLGIAEVLAQRAAQAPLLDELTQPGRF
                     SMLSSDVPIAALGAARLPSAAAQLDLQVAP"
     gene            complement(44174..45601)
                     /locus_tag="PLB86_01020"
     CDS             complement(44174..45601)
                     /locus_tag="PLB86_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRC63748.1"
                     /translation="MPGFVGTTVPAWIDEAVDGGLRTVCLYGSNITDRAQTHRLCRSL
                     RGISTSLLIAVDEEGGDVTRLHFPSGSPEPGNAVLGRLDDVASTRRSAARIGADLAAY
                     GIGLDLAPVVDVNSADDNPVIGTRSFGADPEVVARHTVAYVEGLATQSVAACAKHFPG
                     HGDTHTDSHLDQPVVLADLDTLHRRELVPFRAAIEAGVATVMTSHILVPALDPSAPAT
                     FSEPILQGVLREELGFTGVVVTDALDMAGASATQGIPTAAVRALAAGADLLCLGSDTT
                     PEGYAAVLDAIVAAVDAGVLDPRRLQDAAVRVAALADVHSLAVGDSQPAAGALPSPTA
                     RADVASATIAGTFSVSPSLAAWRAGTGPVAIVQVDTRANLAVGPTNWGPAALGVTIDP
                     GQVPTDARVAVVGRGIGVDHPARAAAAAYGASGHPVVLVECGWPFGDVDVDVDVATYS
                     GSLSVAAALVELLTTSPREALVQPC"
     gene            complement(45645..46484)
                     /locus_tag="PLB86_01025"
     CDS             complement(45645..46484)
                     /locus_tag="PLB86_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRC63749.1"
                     /translation="MTLRRIGRALLTVLTLLLALVWAFPVYWMVNSSMLSNVTLQSTT
                     PTFLPFGGSFDNFAAVFSDPGFLQALGVSLAVTLGTVAASLFIAFLGALAISRFRFRG
                     RKSFILALLLIQMLPAEGLFIAQYKMMSGIGMLNSVLGLTILYTAAVVPFTVWMLRGF
                     VAGVPVDLEEAGMVDGLTRTQAFLRITFPLLAPGLVASGVFAFLQAWNEFTIALVIMT
                     DEQSRTLPLWLRGFVQASASRETDWGQVMAASTVVAVPVIIFFLLVQSRMSSGLVGGA
                     VKG"
     gene            complement(46484..47374)
                     /locus_tag="PLB86_01030"
     CDS             complement(46484..47374)
                     /locus_tag="PLB86_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRC63750.1"
                     /translation="MPVRRRSRLPHILITPTIIVLIAALGYPVAWQIITSFKEFGLRQ
                     QFGVPADWVGLANYVEIVSDPALWAIVLRSLVFCLVVSFATVLIGGALAGLMNSVHKA
                     ARLTLQVALLLAWAMPAVASMTVWIWLVDWRRGLLNWALVRLGVDAQGHNWLADPTSF
                     LIIAAIIVTWMSVPFVTLSIYAGLTQVSPEVLEAAKIDGASGIQRLRHIIVPLIAPVL
                     SIVLLLQFIWNLRVFTQVKILQDAGGVANETHLLGTYIYQLGTGKGDFGSASAVSILM
                     LLLTIAISWRYVRNLMKEES"
     gene            complement(47503..48861)
                     /locus_tag="PLB86_01035"
     CDS             complement(47503..48861)
                     /locus_tag="PLB86_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRC63751.1"
                     /translation="MKKNLQAALVVGTAATLLLTACSGGSTGSAPTATNTAANASKSI
                     TLWLAGGDTPAELRTYLADTFKAKTGATLKIQEQSWGDLTTKLTTALPDANNTPDVVE
                     LGNTQSPTFTNVGAFLDISDMYNELGGTKLLPSFVEAGSVDGKQFALPYYFGSRYLFQ
                     RKDVWTQAGVATPKTLDEFNTAVKTITTANPRKIANFSGFFIGGQDWRNGISWVFANG
                     GDLAKKESGQWVSTLSDPNTIKGLTQLQDIYRNASKAPADAKDASPWLYINDTDQILD
                     DTKAVTGTTSLAAATIMAPGWAHWSIGDLATKDGKPTRQWNDATFGVFVLPGNDGKPA
                     PVFAGGSNIAISNTSKNKDLAKELMRIIFSADFQKKLGGAGLGPANADYVSSLGTDAF
                     ATATIQSASNSKLTPAAPGWAAVESAKVMEEFFGKIRDAADLTALAKEYDAKITPMLN
                     LK"
     gene            complement(48991..50205)
                     /locus_tag="PLB86_01040"
     CDS             complement(48991..50205)
                     /locus_tag="PLB86_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRC63752.1"
                     /translation="MSSVRPQRVRPSAPVQPSHARRQNLSLALQALYSSGPMSRADLA
                     RSLGLTKVTVSELVTALLDRGHAIEVGRSTPGRMGKPSILVDVNRTGLQMIGLDLSDH
                     RALHGAVLDLDGTVVARAQRPVLTDDSVIGDGSTAGPDCPAGPLGGERAVDLVLELVA
                     ECLALATAPILGIGIGTPGIVGSDGVVKTAPNLDWRDVPLRDIVASATNLPVSLCNDA
                     DAAAHADHTLGDGGDDLVLVKIGRGVGCGLIVGGRRVRGAHSAAGEIGHVVVGTDGGR
                     RCRCGKVGCLETWVSAPRLEQALAASTPDPADAAPPADDVPDAVTAAGERLAIALAPV
                     VAALDLADVVLCGPAHVIVPALVESVERTLRARLFTVENATLRVRVVEDPNDIVVRGA
                     AALVLLDQLGVS"
     gene            complement(50280..51164)
                     /locus_tag="PLB86_01045"
     CDS             complement(50280..51164)
                     /locus_tag="PLB86_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HRC63753.1"
                     /translation="MTQVLAEQEWRAAAAAHADRVDALTAGHRERRARGIPHPVEDFV
                     FTYYPFTPSAIRRWHPGLGVRLAGAAADPRAQWRHYRIVDDGVELDVAAYLAARGDTL
                     TFVRDLLTRTADRPPQLGCFGLHEWAMVYRQRPDEVRHSRWPLRLGRAGTDAVVEANP
                     LRCSHFDAFRFFTEPARPLNSLTPTRASQADLDQPGCLHATMDLYKWAMKLAPAIPSE
                     LVADTLELALDLRVLDMRASPYDLRDLGIEPIAVETPEGKATYAGEQRRYAARGQALR
                     MRLLSACASVLAERASID"
BASE COUNT         7714 a        17608 c        17650 g         7937 t
ORIGIN      
        1 acggcttcgt caacggccgc aagctcgaca aggtcggcca ggacgagtcc gacacggctg
       61 agttgttctt cgagaacgtc cgggtgtccg acgccaaccg gctcgggccc gaaaacggcg
      121 gcttcatcgc gatgatgcag cgcctgcctc aggagcgggt cggcgccgcg gtctccaacg
      181 tggctcacgc gttccagatc ttccgcgaga ccctcgtcta caccaaggag cgcaaggcgt
      241 tcggccagtc catcggggcg ttccagcaca acaagttcaa gctcgccgag atgctgacca
      301 agctcgaagt cacccaggcc tttgtcgacg actgcgtcga cgcccacgcc cggcacgagt
      361 tgtcgtcggt cgatgcgtcc aaggccaagt ggtggtccgc ccaggtgcag aacgacgtcc
      421 tcgacgagtg cgtccagttg cacggcggct acggcttcat gaacgagtac cgggccggcc
      481 gggcgtggcg cgatgcccga gtcaccatga tctgggccgg ctccaacgag atcatgaagg
      541 aactcatcgg ccgtgacctc ggcctctgag tcggcgtccg acccaccgtc gaacgttcct
      601 gtcatgggtt ctcacacggg agcagtcgcc atcgtcaccg gcgccagccg gggcatcggc
      661 ttcgggatcg cgcaacggtt ggtcaccgag ggcgcccgcg tcgtcatcac ggctcgcaaa
      721 cccgaacccc tcgccgaggc ggtggctgcc ttgggcggtt ccgagtatgc cgtcgggctg
      781 gccggtaacg ccgcggacga ggctcaccag gacgaggcga tcgcgttggc tcgcgctgcg
      841 ttcggggggc tggactacct cgtcaacaac acgggcatca acccggtcta cggcccgatc
      901 ctcgatgtcg atgtcgacgt ggtccgcaag gtgctcgacg tcaacgtcgt tgccgccttc
      961 tcgtggatca agaaggtcgt tgccgctggg ttgggctcgc ccgaccaccc agggtcagtc
     1021 gtcaacatcg cctcgatcgc cgggcttcgg gccagcggga ccatcgggtg gtacggcgtc
     1081 tccaaggcgg cgctcatcca cctgacgatg gagttgggcc atcaactcgg tccggacgtt
     1141 cgggtcaacg cggtcgcgcc cgcagtcgtc aagacccagt tcgctcaggc gctctatgtc
     1201 ggccgcgagg agaaggtggc cgcggcatac cccctgaagc ggctcggcgt cccggaggac
     1261 atcgcgggag cggtgagttt cctgctgtcc cgcgatgcgg cgtggatgac cggccagacc
     1321 ctcgtggtcg acggcggcgt caccctcggc ggcgctctct agccattggc ctccggcact
     1381 tggccgcggt ctgatgcgca cctgaccccc tgacgcgcac cggggccgat tgggcacacg
     1441 aagcggtccc gagacccctg gactcgggac cgcttcgcgc cccaggcggc gccgtctcgt
     1501 ccgggcgcgc acccggccga tgcggcgcgc atcgatggcc tggcaccttc ccgaagccct
     1561 acaccgtgca agagcaccgt cgcgcggccc tgatacatcg gggccgtcgg tgcgcagacc
     1621 taggatcgac gggagccggg tgaccggcat accgtcgcag cgagggagga accgccgtgg
     1681 gtgaggcgag ctcccgagcc gacgacatcc tggccgccct cgaccccgaa cagcgtgagg
     1741 tggccgccag cccagtcggc ccgatgtgtg tgctggccgg cgcgggcacc ggcaagaccc
     1801 gggccatcac ccaccggatc gcttatggcg tccactcggg ggcctatgtg ccgcaacggg
     1861 ttctcgccgt gacgttcacc gcgcgggcgg cgggggagat gcgcacccga ctgcgcgagc
     1921 tcggcgtcgg gggagtgccg gcgcgcacct tccacgccgc ggcgttgcgc caactgcact
     1981 tcttctggcc gcaggcggtc ggcggcgcgg ccccggaggt gctcgccagc aaggcgcccg
     2041 ttgtcgctga gtccgtgtcg cggttgcgga tgcagttcga ccgcaccgcc atccgcgacc
     2101 tcgcggccga gatcgagtgg ggcaaggtct cgctgctgac cccgcagtcg tatgccgcgg
     2161 gcgcccggcg ggcgggccgc gaagcgccgg gcatcgacca cacggcgatg gcccgggtgt
     2221 gggaaagcta cgaggagcgc aagaccgagc gcggggtcat cgacttcgag gacgtcctgc
     2281 tgctcatggc cggcatcctc gacgagcgga tggacatcgc gcgcaccgtc cgcgatcaat
     2341 atcgacactt cgtcgtcgac gaataccagg acgtcaacgc cctgcagcag cggctgctgg
     2401 acctgtggct gggcggtcgc gacgagttgt gcgtcgtcgg cgacccggcc cagacgatct
     2461 acaccttcac gggagccacc ccggagcacc tgctgacctt ccgcagccgc tacccgcagg
     2521 ctcggctcgt ccggctggtg cgcaactacc gcagcacccc ccagatcctc ggcctggcca
     2581 acctcgtcgt ccgctcggcc cggccgtcgc tgcgccgccc gggtgtcgga gggtccgcgg
     2641 cggcccgagc gctgcccgat cacgtcgacc tccaggccca aggtgagccg ggcccgcaac
     2701 ccaggctcac ggcatacccg gacgatccgg ccgaggcggc gggtgtggcg ggcgcgatca
     2761 aggccctggt cgacgggggg cgaccgccgg gcgagatcgc ggtgctgttc cggaccaatg
     2821 cgcagtccga ggggttggag tccgcactcg ccgcgctgga tgtgccgtat ctcgtccgcg
     2881 gtggcgagcg gttcttcgcc cgacaggagg tgcgcggggc catcgtgctg ctgcgtggcg
     2941 cggtgcgcgg tgacgacggg tccagaccgc tccctgagct ggttcgcgac gtgctcaccg
     3001 gcgtggggtg ggccgcgacc ccgccgacgt cggggggagc gcagcgcgag cgctgggagt
     3061 ccctcatggc cctggccggg ctcgccgatg acctcgtggc agccagcccc ggcgcgcgga
     3121 tgggcgatct cgtccgtgaa ctcgacgagc gggccgcggc gcagcacgcg ccggccgtgg
     3181 ccggggtgac cctcgcgtcg ctgcacgcgg ccaagggcct ggagtgggac accgtcttca
     3241 tcgtcggctg ctccgacggc ttcctgccca tcgtcatggc cgagggtgag gtggccatcg
     3301 aggaggaacg ccggctcttc tacgtcgggc tgacccgcgc gcggcgcgac ctgcagttga
     3361 gttggtccgg ggcgcggacc ccgggggcgc gggccacccg gcgcccgtca cggttcctcg
     3421 acccggccgc gtcgatcctc ggggaggggg cgcgctcggc cccgcggcgc tcgggggtgg
     3481 tcggcgggtc cgggtcgtcg cccagcgctc ctcgccagcc tcgactgcct tcggtatgcc
     3541 gcggctgcgg caccgagttg accactgcgg ccactcgcaa ggtcggcagg tgcgaggact
     3601 gcccaccgac ctacgacgag gcgaccttcg aggccttgcg ggtgtggcgg ggcgaggtgg
     3661 ccaagaccat gaaggtgccg gcctacgtcg tcttcaccga tgccacgctc accgcgctcg
     3721 ccgagaccgc gcccaccgac ctgcgcgggg tcgccggggt ctccggcgtc ggggccatga
     3781 aactcgaccg ctacggcggc gccgtcctcg acgtgttggc cggcggcgat ccccatatgg
     3841 ctgccgaaaa gactactgcg gcaatgggtt ccgacaactc gtgacggtcg cgccgaatgc
     3901 gtccaaactt ttttcattaa ttcgtttgtc cccggccgcc gccgagggtt accctcgtga
     3961 acgcacaacc tgcggggccc gaagggccca ggcaggacga cgagagcaag gaaggagggc
     4021 ggctttgatg gagatcaaca ccatgatcac gcgcacgacg gcgcgactgg gcgacagcgg
     4081 aatcgggtct gaccacctcc tgctgcccac cgtcccggcc gttcaagggg gcactctgcc
     4141 caccccgcgt cgcgttgtca tcgccggccc ggccgctgtc gccagcaatg gcgagggtgt
     4201 ggcctttgcc gccgcttacg ccgccagccc caagaccgat gtccaccgtt ccgccaccgg
     4261 gagcccaccc gtctaggccc acctagaccg gactcacctc caggccgcgg aacccgacaa
     4321 gggatccgcg gccttcgtgt ttgtcacggg ggccgacacc ccgaaggcca ctgagatgaa
     4381 caccctgaga tcgcgccaga ccgccagacc gcaggacgaa caacgtgccc gggtccctca
     4441 ccagggatcg cccggacaac aggacagaac ggcccccgcc accgcggacg gccgacagga
     4501 ggaggaggag gtgacaccga tgcagctgag caccctcatc gacacccaga cggcagcgat
     4561 gtccgtcggc gtcgggatcc cgtgccgtga ctacgacgcg gagctctggt tcgccgagcg
     4621 cccggcggac gtcgagttcg ccaagtcgct gtgcggcacc tgcccggcgc gcaccgcgtg
     4681 cctcgccggg gcccttgagc ggggcgagcc gtggggcgtc tggggcgggc aattgctcgt
     4741 ccaaggcacc atcgtcgccc gcaagcgtcc tcggggccgt ccgcgcaaga acgacgtcgc
     4801 ggcgtgaggc agggggcggg cgctcccgat cgcatccggg agcgcccgca aggcgcccgg
     4861 ccgtcctttc gctcgtcgca cccgctccac ccgtcatacc cagacctcgt cctgaagggg
     4921 actaccacca tgtatcaacc agtccacgag cagctggctc gtgcccgtca tgccgaacgc
     4981 gaggcccgaa ccggccaggt cggcatccgg cgcagcgccc ggctgatctc catccagatc
     5041 cggcgccaac gccggacacc gcacgcctga gcaggcctgc ggaccgtcga tgagggcggg
     5101 gtgaccgacg accggtcgcc ccgcccttgt gtgtggtccc ggcggagggg tttgcagagc
     5161 gttgcccaca gtggactgtc gccaacgctc cgcgaaggga ctggctgccc cgcgaggcga
     5221 cactcccgcg ggtggtggca cgcggcatac agtgacctcc gtgcaggcca acttgaccat
     5281 cgcccaggac cccgaggccg atgccctcat ctccagggac ccgttcgccc ttctgatcgc
     5341 catgctgctc gatcagcaat tcccgatgga gagggcgttc gccgggccgg ccaagttgct
     5401 ggctcgcttc ggcaccctcg accccgttcg tgtcgcggag gctgaccccg aggctttcgc
     5461 cgacctggcc gccaccccgc cggcgatcca ccggtacggc cgggccatgg ccggacggat
     5521 ccagcaggtg gccgcacatg tggtcgagca ctacgacggg gacgccgagg ccatctggac
     5581 cacggccaaa ggccccgacg agctcatccg tcgcctcgtc gccctccccg gcttcgggga
     5641 gcagaaggcc cgcatcttcg ctgccctgct gggcaaacaa ttgggcgtgc gcccccgcgg
     5701 gtggcgcgag gccattggcc cgtatgccgc gcccggcacc ttccgctcgg tggcggacgt
     5761 cgtggacgcc gagtccctgg ccaaggtgcg agcgttcaag caggcggcca aggcggcagc
     5821 caaggcgagt gcccaatgag cgaggcgccg gctcttggcg tggtcttccc tgtcgagggc
     5881 gacggccgga gcacgagtgc caccgggcgt gccgtcatcg ctgatgcgct tgaggtcgtc
     5941 gacccggtgg gagcgcgggc ggctcgtgac gaagccaact ggcgcaccgg ctatccattg
     6001 cacttccggc gcctcgttga agccgggctg ggcaccgccg ccgatgcggt cggaatcgcc
     6061 gatgccggac tgacttccgt ccacagccgg atgcgatgga gccgtccaga gggcaccgag
     6121 gtcgctctgg gcgaggcgct ctcgggcgac ctgcgaccgc tggagaccga tcggctggcc
     6181 gggtcgggtg agccggaggc ggccttctcc ctgccgtatc gcggggctcg gctgtccggc
     6241 gacgcggcgg tcgcccaggt ccacgactgg gtgtcgcgag ggatcatcga gccgtcctgc
     6301 ggcgacgccg tggagcaggt catcgcccat cccgaatggc tcgacctctc ggacttcacc
     6361 ttcgtcgtgc tgggcgcggc cgcggagatg ggaccgctgc gcgcgttgct gcgctggggc
     6421 gccgatgtgg tcgcggtcga cttggcccga ccagacctct ggcgcggcct gttgtccgac
     6481 actcgccggt tcggtggcac cctgcgcatc cctgtggcgg ccgggtccgc gcccgtcgag
     6541 gagcgcgccg gtgtcgatct ggtcaccgag tggcccagcc tgtctgcatg gttggtggag
     6601 cagccgggca ccctcgtcct gggcaactac gtctatgccg acggcgccga caacgtgcgg
     6661 gtcagcgtgg ccgtcgacgc cctctcgatg agcctgcgcg cccaacgccc ggacacggcc
     6721 ctggccttcc tcgcgacgcc gacggacgtg tttgctgtcc cgccggccga cgttgagcgg
     6781 gcgaccgcgg catacgcgaa tgcccgcgtg agcaaagcgc tgcgggggcc gctgcgtggg
     6841 ttgtcgggtg ggcggctgct cagccgcaac tacgcaccgc aagccaaccc tgggatcgct
     6901 gacgcgatcg tgccccagca gggccccaat taccttctcg ccaaacggat ccagcgatgg
     6961 cgggcgacta ctgcacgcgc ggagggcgcg accgtgtcga tgaccgtcgc cccgccgacg
     7021 cggacccggt cggtcgtcaa gaaccgggcc ctggccgctg cctacgccgg tgctcatcgg
     7081 ttcggcatcg aggtgttcga cccggcgacg gccaacaccc tgatggcggc tgtgctcgtc
     7141 catgacctgc gcaccgggac gaagaccttg ggtcatccgt ggttggacga ggcgcaccaa
     7201 gccgcacacg gcggtctgtg gacggcggca tactccgcgc gaagtgcgtt ggggctggcg
     7261 gccgtgctcg gtctgggctc ccggtcgtga ccgtggccga ggaccggatc gtctgcatca
     7321 gttgcggcag tatgccgtcc cctgacgacg aggggaccgc acgggtcgag tgggtgcggg
     7381 gcgtcgagaa cgggcgtccg gtgtggacct gccccgaatg cagccgacgc aacctgcgca
     7441 gcatggaagg caaactcgac tccgcgtggt ggtgagtcgt tgttgactga ccctgacctc
     7501 gccgaccgac gcggacggga aagcgggtca acccggcgtc gacccgcggt cagatcagcc
     7561 cgtcgcgcag ctgggagatg atccgcggcc agtaggtgtt gcgtttcaac gcatccgggt
     7621 ccgcagcctt gaaggtgtcc cgcaactcgt cgatggcctt cttgcggttg atccggggtt
     7681 tgggctcggt cttgggcttc ggccgcggct tcagggtgcc ttcatgcaac tggcggatca
     7741 tttccgcggc ctcctcaggg ccgatgatcc ggaacgagct ctccgcgggt ttgagggcgt
     7801 cggagacggg tcggtagtgc tcgaggaagg tgatgaaggt ctggacgctg gagttgatga
     7861 aggcgttgtc ggcgggttcg gggccgtagc cgacgacggc gagggtgtcc aggtcgatgc
     7921 cgacgcggag gtgttcgggc tcccattgtt cgtagccgat atcgaggagc cgtggccaac
     7981 gcttggtgct ggcggcgctg ggggtggggg tgacgcggaa ggggtgttgg aaggtgaccc
     8041 cgagggtttc cccgtcgaga acgggcaggc cggtggtggt cagccagtcg tgcagggtgg
     8101 gaggcaggtc cagggcggcc gcctcgtcgg gtgtcaggcg gatgcgatcc gggccggcgc
     8161 ggtccgcggt cccggatgtg ggcggcatac ccatgtcagg gtcgcctcgt tgcggtgcgg
     8221 tggtggcccc ccgagggcct gttcggccga gtgctttgca tgggcgggca cgctatcagg
     8281 ggccctgacg cgaagcgatg gcccgctcgt cgcgctgcgg cggctggagc ccgctcactc
     8341 ctcatcgacg ggcggcaggt cagcaccggg gatgtgggcc gcaaccttcg ctcgaccagg
     8401 gaccgtcgca ccgagttggc acagcacgcc gatcccgccg agccagaccc ggtggatgag
     8461 gagatattcg gcgggcaggt tgagccgcat ccccacggcg tagttgggtt tgcgcgggtc
     8521 gttgatgtgg gcaaagacgt cccggatcca gtcgcgggag aagtggaaag tgtccgtgcg
     8581 taggggatcg atgaacggct gaaggtagtc cagcaggcgc tgggcgtcga cgtcgatccc
     8641 gtcacggacg aacccgatct cgcgcaaacc cgcgagggtg gcacctgcgt cgtcgtccag
     8701 ggccaacgtg agcagacgtc ccatgtcgtc gggcaggccg ccgggcaggt gcttgacggc
     8761 gccgaagtcc agcaccccca gccggccgtc ctcgagcagc cggaagttgc ccgggtgcgg
     8821 gtcggcatgg agcaagccgc atcgcccagg cgcgtcgagg aggaactcga tgtagtgcgt
     8881 cccggcaagg tcacgctgtt cctgggtccc gtcggcgatg atgctcgaca ggggtatgcc
     8941 gtccatccac tcggtcacta ggacgcgctc ggtgccgtcg atgacaccgg ggacggccac
     9001 gttcgggtcg ccctcgaaac ccttggcgaa ggccttctgg gccgaggctt cgagccggta
     9061 gtccaactcc tcggacatcc gcccccgcaa ctcctcgagg atcgggccga tgtcgatccc
     9121 ggggatccac gaggtggcca accgggccac tttggacacg ttagacaggt cggacagcag
     9181 cgcctttccg gcccccgggt attgaatctt caccgcgacg tcacgtccgt cacgccaagt
     9241 ggcccgatgg acctgaccga tcgaggcggc cgcgacggcc gtttcgtcga agtcgaggaa
     9301 tctccggcgc caacgcggac cgaattcggc ggccatcact tccttgaccc gagccggcgg
     9361 catggctggt gcgctgtcct gcagtttggt gagcatggcc cggtagggag cggcagcctc
     9421 ctcgggccac gcggcctcga agatcgacag cgcctggccg aatttcattg cgccaccctt
     9481 gagttcgccg agcacggtga acagttgagt ggcggcccga gcctgcatct cggcggcaac
     9541 ggcctcggcc ggcttgcccc cgacccgttt gccgagaccg agagcggcgc gaccggcata
     9601 ccccaggggg agcgtcgcga gcttggcggc gcgggtcact gccgccctgg ggagatcggt
     9661 cacgcgctca ttgtctcccg gcggctgcgg cgctgccgac caccgccccg atggtgggcg
     9721 tcggcgcgac agccctccga tggtgggggc actgaggatg gcggggccac ttgcggtggt
     9781 ccaaccgcgg ccacggggag gtgagctcga ccgtcacccc atcggggagg cagtccccgc
     9841 cgaggtatgc cgcgaccacc agcgccgcga ccgcggccgc cgcggacagc agggctggct
     9901 ccatcggggt gggccggacc gggccccctg ggccgaggag tgccgtcagc ccctcggtcc
     9961 actgtcgtgg atcggcaagg gctgcgggcg gccggcttgt ctgggccaac acggatgccc
    10021 gccccgagtc gcggtcgcag cggtgcagtt ccaggcattg gatacacgtc gtgtccccgc
    10081 gtggcggcgg cacgacgagt ggaccgacga cgacccggtc gccctcggtc gtgaaggcca
    10141 ggtgagcgat gccatgccta gcccagggcg ccgcctccgc ggggtcgagt ccgtcaaagg
    10201 aactcagcag gaccagatcg ggtgggcgct gccggcccgc gacggtgtgc tcgcggtcgc
    10261 gcagggcaag ctcggcctga tccagggcca acggccccac ccgaaccccc tcgaggcccg
    10321 cctgccgcag cgcgcgggcc aggcgatcgg tgagcgcgtc cggaccgccg acaagcaccc
    10381 gaggttcgct cccgagtcga gccgcgagga gatcgacgcg ctccgaccgt ggatcggcca
    10441 tcgcgcccag ccgacccagc agggtgagca actcgtcgac gacgtgtggt gggtcaccga
    10501 aatcggctgc gacatcatga agttgggcga gcgaccggga cccgtccagg cgctcgagaa
    10561 cggcgacctg accttccgac agcccgcgca acacgagggc ttgggggccg acccccaact
    10621 gcacctcgtc gacgccgcga cgcagcggag ggcgacgcgg atgcagccgg ggctggctgg
    10681 cacgcgccga tcgatccggt cggggacctc gctgcgacat gctgcgaatc tgccacagca
    10741 ggggccaggt ccgtcagcgt catccacagg cccgcgaatg ccggagtgtg cgtgccggga
    10801 ggtgccggac gggcgacccg gtcagaatgc acggagggca acccgcgttg cgggtcgccc
    10861 tccgtgtcct gtcgagctgc ggatcaggcc ttgcccagga tccggttgag gttggtgccg
    10921 cagaccgggc acttcgcctt cgccatgcgg cggccgttct cgctgacaac gaccttgccc
    10981 tcagcggtcc tcttctcctt gcacttcacg cagtagaact cgcccgcgta ggtctcgtcg
    11041 ttggccatgt gtggtcctcc tggtcggggt gtggggctcg tgactccgcc catcggcggg
    11101 tggcatcccg gacaacccta gaccagcgaa ccgggtggaa cgtgaattga gcccttcccg
    11161 cacgtcggcc cgtcgtgcct gccgcaggtg ggctggttgg caacatggcc agaagtgtgg
    11221 gccacagccc ggccccgtcc agccccctct gtcggtggcg cgtcgtatcg tgggcggtat
    11281 ggacctggac gtggcgctgc cgttcgaggt cgatgctggg cactcgcaat ccccggtgga
    11341 cactcctgct gtgccgacca gagggcggcg ggcgacgagc caggagcagc cgctcgtcga
    11401 ggtgcggcgc agcacccgac gccgtcgcac cgtgagcgcc taccgcgatg gcgaccgggt
    11461 cgtcgtcctc gtccccgccc ggttcacccg ggccgaggag acgcgatggg tccagacaat
    11521 gacggagcgg ctggcggcca gcgaggccaa acgtcgtcct agcgacacgg atctggcggc
    11581 ccgggctgag gcgttgtccg agcggttcct cggcggtcgg gccaggcccg catcgatccg
    11641 gtgggtggcg aaccaacgcg gtcgctgggg gtcctgcaca ccgtccgagc gcacgatccg
    11701 gatttcctct cgcgtccaag gcatgcctgg ctacgtgttg gactacgtca tcctccacga
    11761 gctcggccac ctattggtcg ccggtcacgg gcccgacttc tgggccgtgc tgtcgagcta
    11821 cccacggctc gagcgggccc gtggttacct cgacggggtc gctgccgctt ccgggctggc
    11881 cttgagcgac gattgatccc gtgatggctc gcacggccgc ttcatcgccg gcccgccccg
    11941 gtcgccgcag acgttcgccc ggctgggttc cgacccaaca cggagcctgg gcgatgctcg
    12001 tcagcccgtt gctcctcggc gcccttgctt cgggcccggg ctggatccac ctgctgctgg
    12061 cggcgttttg gttcgcgggc tacttcgcct tcaacgccac gagcctctgg ctcaagtcgg
    12121 gccgcaaggt cagatacctg cgtccggtcc aggtgtatgc cgccctcgcc gccgctctcg
    12181 ggctggccac ggcgctggcc gatcccggct tgctgcgctg ggcgcccctc ttcgtgctgc
    12241 ccttgggggt cggcgtctgg gcagccgccc accgccggga gcgtgacctg ttggccgggg
    12301 tcaccacggt catcgggtcg gcgctcatga cggtcgtggc ctatgacgcg ggtggtggtc
    12361 ctgatctgca ccgggccgca gtgctgggcg ccgggcagtt cctctacttc gcgggcaccg
    12421 tcttctacgt caagtcgatg atccgcgaac gggacaaccc ggcatacctg cggctgtcgg
    12481 tgggcttcca cgtggtcgcg gccctcgcgt tagcgccgtg ggctccatgg gtggcggcgc
    12541 tgttcgtggt gctggcactg cgggcttggt gggtgccgcg cctggggtgg acgcccaaac
    12601 agatcgggat cgccgagatc gccgggacca ccgcggtcgc gctcgtgtcg ctcttcacgg
    12661 tctgagcccg cactaacctg gggactgaac acccgtcaag agaggtcgac catgccgcag
    12721 ctgcgttccc ggacaaccac ccacggtcgc aacatggccg gggccagggc cctgtggcgc
    12781 gcgaccggcc tcaccgacga cgacttcggc aaacccatgg tggcgatcgc caacagctac
    12841 acgcagtttg tgccgggaca tgtgcacctc aaggacctcg gtgacctcgt ggccggggcc
    12901 gttcgtgagg cggggggagt ggctcgggag ttcaacacga tcgccgtcga cgacggcatc
    12961 gccatgggcc accacgggat gctctacagc ctgcccagcc gcgagatcat cgccgactcg
    13021 gtcgaataca tggtcaacgc gcacgccgcc gacgcgctcg tctgcatcag caactgcgac
    13081 aagatcacgc cgggcatgct gctggcggcc ctgcggctga acatccccac cgtgttcgtc
    13141 agcggtggcc cgatggaggc cggcacggcg gtcatcggcg aggacggcac cgcgaccacg
    13201 aggctggacc tcatcgatgc gatgagcaag gcggtcgacg accacgtctc cgatgccgac
    13261 atcgcgacga tcgagcgctc cgcctgcccg acctgtggct cctgctcggg catgttcacg
    13321 gccaactcga tgaactgcct cctcgaggtc gtcggcctgg ccctgccggg caacggctcg
    13381 accctggcca cggccgccgc tcgcaagggc ctcttcgaga gcgccggtcg cctcgtcgtc
    13441 gagctcgcca agcgttggta tgccgtggac ggcaccgagg acgcgagcgt cctgccgcgc
    13501 tcgatcgtca cccgcgaagc gctgtccaac gcgatgcgcg tggacattgc catgggcgga
    13561 tcgaccaaca cgatcctgca cctgctcgcg gctgctcagg aggccgacat cgccttcgac
    13621 caacacgaca tcgacgccct ctccagggtg actccgtgcc tgtgcaaggt cgccccgaac
    13681 accaaccgct actacatgga ggacgtccac cgggccggtg gcatcgtcgc gatcatgggc
    13741 gagttgaacc gggccgggat gctcgacgac tcggtgcatg cggtccacgc tccgtcgctg
    13801 caggcctggc tcgatgagtg ggacatccgc ggggggaaga cgagcgacgc ggcatacgag
    13861 ctcttccacg cggcccccgg cggagtgcgc acgaccgagg cgttctcctc gaccaaccgc
    13921 tgggcgaccc tcgacaccga cgcacgcgaa ggctgcattc gctcggtgga gcacgcctac
    13981 accgtcgatg gcggtttggc cgtgctgact ggaaacctcg ccccggacgg ctgcatcgtc
    14041 aagacggccg gtgtgcccga acaccagtgg accttccgtg ggccggccaa ggtggccgag
    14101 agccaggatg ctgccgtcga gatgatcctc gccgggaacg tggtcgccgg ggacgtcgtc
    14161 gtcgtccgct acgaaggccc caagggtggg cccgggatgc aggagatgct ctacccgacg
    14221 tcctacctca agggcgtcgg cctggggccg aagtgcgcgc tgatcaccga cgggcggttc
    14281 tcgggcggct cgtcggggtt gtcggtgggt cacgtgtcac cggaggcggc ctcccgtgga
    14341 gccatcgggc tggtccgtga cggcgatgag atcgagttct ccatccccga gcgccgggtc
    14401 acgctgcacg tgtcggacga cgaactcgac cgtcgacggg tggagcagga cgccgcaggg
    14461 tggcagccgg tggcccggga gcgggaggtc tcggcagccc tgcgcctgtt cgcgctcttt
    14521 gcccagtccg ccgacaaggg agccgcccgc aaggtcccgt gaggtctgcc cgcctggtcg
    14581 agggcaaact tgacgaggtg ctggagttgt tgcggtctcg ctgagcaccc gccgagatac
    14641 cgggcgccgc gcgagctcca cggcagaatg cccgcatgct ccgtcgcctt gctctcgtcc
    14701 gtcagccgtc cccgcgcctg gccgacggca tcgtcactca tatcgaccgc gagagcgtcg
    14761 acccggtcct cgcccgcgac cagtgggagg cctacgtcgg tgccttgcgg gccaacggct
    14821 gggacatagt ccaggcgccg gcggccccgg actgtcccga cggggtgttc atcgaggacc
    14881 aggtcctcgt ctacggcgac ctggcgctcc tgtgccgcag cggcgctcca gaacgccggg
    14941 ccgaacagga cggcctggcc ggtctcctga ccgacgtggg ctaccgggtg gctcgcatcg
    15001 aggcgcccgg cctgctcgac ggaggtgacg tcctcaaaca cgacggtcga gtctgggtcg
    15061 gcgacgcggg atcgacgggc aggtccaacc gggccggcgt cgagcaggtg gccgacctgc
    15121 ttgccccgct gggtgcccgc gtcgaagcgg tgccgttgtc cggggtcctg cacctgaaat
    15181 cggccgtgac tgcgctgccc gacggccgca tccttgccgg ccccagcctg agcgccgtca
    15241 ccgcagaatg gcccggctgg ggcaccagct cgaggggtgc ctcccgcctc ctggcagttc
    15301 cggaggagtc cggagctcat gtcgtcatcc tcgacgccga caccgtgctg ctggcggcct
    15361 cggcgccgca gaccgccgag gtgttgaccg gccagggctt gcgggtcgtc accgtcgaca
    15421 tcagcgagtt cgagaagctt gagggctgcg tcacctgcct gtcggtgcgc ctgcgccgct
    15481 gacccaactc gcaggtgccc ggcgtgaact ccgtggctcg gatggtgaga cgaggcccgt
    15541 ccgcggttga cacggcatac ccccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnccgtg gctcggatgg tgagacgagg cccgtccgcg gttgacacgg catacccccg
    15721 caaaggaccg tggtcttgtg acccaggtcc tcgtactcaa caggcgcgtg tgacgcctct
    15781 gtagtcacgc gcgcgccctt ccgtcctgcc ggtggactga agggcttttt cgtgggacga
    15841 gaaccccacc ggcatcagtt cgcacgtccg acgcaacgaa gaagtggtga acaccgtgac
    15901 cgacagcctg acgtccacga aggcgtcccc ggccgacctc gctgcccaga atcggcagcg
    15961 cccgagcgcc ccggcggccc ctgcggtccc ggccgttccg gagccctgca cgggtgccca
    16021 gtcgctggtc aagtcgctcg aggccatcgg cgtcgacacc gtcttcgggc tgcccggcgg
    16081 cgcgatcctc cccgcctacg accccctgat ggactccacc aaggtccgtc acatcctcgt
    16141 gcggcacgag cagggcgcgg gtcacgcagc cgaggggtat gccgtggcgt ccggccggat
    16201 cggcgtgtgc atggcgacct ccggcccggg agcgaccaac ctggtcaccc cgatcgccga
    16261 cgcctatatg gactcggtcc cgatggtcgc catcaccggc caggtgaaca gcgccgccat
    16321 cggcacggat gccttccagg aggccgacat ccgcggcatc acgatgccga tcaccaagca
    16381 caactacctc gtgaccaacc ccgacgagat ccctcgggcg atcgccgagg ctttccacat
    16441 cgccgggacg ggccgtccgg gtccggtcct cgtcgacatc agcaaggacg ccctcaccgg
    16501 gtcgacgacg ttcagctggc cgccggtcat cgacctgccg ggctaccgtc cggtggcccg
    16561 tccgcacggc aagcagatcc gtgaggcggc tcgctacatc gccgctgccc gtcagccgct
    16621 gttctacatc ggcggcggcg tgatccgggc tcgcgcctcg gaggtcctgc ggaccctcgt
    16681 cgacctcacc cagatcccgg tcgtcaccac gctcatggcc cgcggcgcgg tccccgacag
    16741 ccaccctctg cacctgggta tgccgggtat gcacggctcc gtggccgccg tcaccgccct
    16801 gcagaagacc gacctcatca tcgccctcgg cacgcggttc gacgaccggg tcaccggcca
    16861 gttgtccacc ttcgcgccga acgccaaggt catccacgcc gacatcgacc cggccgagat
    16921 ctccaagaac cgcaccgccg acgtgccgat cgtgggcgac tgcaaggacg tcatcaccga
    16981 tctcatcgag gcgatcaccg ccgagcaggc cgcccacggc gccgccgagt acgaggcctg
    17041 gcaggcgacg gtggccgagt ggaaggagac ctacccggtc ggctacacca cgaccccgga
    17101 cggtctgtcg ccgcagtacg tcatcgagcg gctcggtgcg ctgtccgggc ccgaggcgac
    17161 ctatgtcgcg ggtgtgggtc agcaccagat gtgggcagcg cagttcgtgc agtactcccg
    17221 gccgaattcg tggatcaact ccgggggcct gggcaccatg ggcttcgccg ttccggcagc
    17281 catgggcgcc aaggtcgccg agccgcaccg cacggtgtgg gccatcgacg gtgatgggtg
    17341 cttccagatg accaaccagg agttggccac ctgcgtcatc aacgacatcc ccatcaaggt
    17401 cgcggtcatc aacaactcga gcctgggcat ggtccggcag tggcagacgc tcttctacaa
    17461 cgagcgctac tccaacaccg acctgcacac gagccacaac cagcagcgca tcccggactt
    17521 cgtcaaactc ggcgacgcct acggctgcgc gagctggcgg gtcgagcggc ccgaagaggt
    17581 cgacgaggcg atcatcaagg ccttggagat caacgaccga cccgtcgtca tcgacttcgt
    17641 cgtccaccgg gacgccatgg tgtggccaat ggtgccggcc ggcgtgagca acgacaagat
    17701 cctcatcgcc cgcgggggag cccccctgtg ggagcgcgaa gcctgagccg tccgcagacc
    17761 gcccccacca gcctccccag ccgcagcagc acagagagac gccgaaatgc ctaagaacac
    17821 cttgtccgtc ctcgtcgaga acaagcccgg tgtgctcgcc cggatcgccg ccctgttctc
    17881 ccgtcggggg ttcaacatcg acagcctggc cgtcggcccg accgagcacc cggacgtgtc
    17941 ccggatgacc gtcgtcgtcg acgtcgatga gctgccgctg gagcaggtga ccaagcagct
    18001 caacaaactc gtcgaggtcc tcaaggtcgt cgagctcgag ccgcagtcgt cggtccagcg
    18061 ccagatcgtc ctggtcaagg tgcgtgccga cgcctcgaac cggtcgcaca tcgtcgagac
    18121 cgctgagctc ttccgggcca aggtcgtcga cgtggcgtcc gacagcctga cgatcgaggc
    18181 gaccggcaag gtcgagaaga tccaagcgct gctcggtgtc ctggagccct tcggcatccg
    18241 tgaactcgtc cagtcgggcg tcgtcgcggt cggccgcggg ccccgatcca tcaccgaccg
    18301 agccctgcgt agcgcctgac gcagcctcac tcgaggtgac cgcccgctgg ccgagcggcc
    18361 cgcggggtga gcgcacggca taccctctag tcccaactgc accacccacc gaccgcacga
    18421 accagctaag gagtggccct ctgatggccg acatgatcta cgacgacgac gccgacctga
    18481 gcatcatcca agggatgaag gtggccgtca tcggctacgg cagccagggc cacgcccacg
    18541 cgctcaacct gcgtgactcc ggggtcgacg tgcgcgtcgg cctcgccgag ggcagcaaga
    18601 gccgggccaa ggccgaggcg gaggggctgc cggtcaagtc ggtggccgag gcgaccaagg
    18661 aggccgacct cgtcgtcatc ctcacccccg accaggtgca gcgccacgtc tactccgacg
    18721 agatcaagcc caacctcaag gagggcgcgg gtctgctgtt cggacacggt ttcaacatcc
    18781 ggttcggtta catcacgccc gagccgggct ccgacgtcgt catggtcgcc cccaagggcc
    18841 ccggccacct cgtgcgccgc gagttcaacg acggccgtgg cgtcccggtc ctcgtctgcg
    18901 tcgagcagga tgcctccggt cgggcctggg acatcgccaa gtcctatgcc aaggcgatcg
    18961 gtggcctgcg cgccggcggc atcaagacga ccttcaccga ggagtgcgag accgacctgt
    19021 tcggcgagca gtccgtgctg tgcggtggcg cgtcgcagtt ggtgatgtac gggttcgaga
    19081 ccctcgtcga ggccggctac cagccggagg tcgcctactt cgagtgcctg cacgagctca
    19141 agctcatcgt cgacctcatg gtcgagggcg gcatcgccaa gcagcgttgg tcggtgtccg
    19201 acacggccga gtacggcgac tacgtctccg ggccccgggt catcgacccg agcgtcaagg
    19261 acaacatgcg cgcggtgctc accgacatcc agagcggtgc cttcgcccgt cggttcatcg
    19321 ccgaccagga cgctggggct ccggagttca aggcgctgcg cgccaagggt gctcagcacc
    19381 cgatcgaggc gaccggccgc gatctgcgca agctgatgag ctggatcaag gagacggact
    19441 ccgactacac cgagggctcc gctgctcggt gacccttcag tcaccttccg tgacctttca
    19501 atcacgggcc tcaggtgggc tagcctgacc cgatgatctt tcccagcgag gtatgccgcg
    19561 tgcgcgagat gcgccgcggc atacccgttc ggtgacgtgg tgttcgacag cctgcccttc
    19621 ccgctggtct atgcggtgtt cttcctcggc gccatgattc gggccaacgc gacctactgg
    19681 ctggggcgcg gcgcccgcaa gggcggggag accacccgcg cggcgcgacg gctgcacagc
    19741 ccggccattc ggcgagccga gacggtgatc tcgcggttcg gggccccggc cgtggcgctg
    19801 tccttcctca cggtcggagt ccagacagcg ctcaacttcg ccgccggcgt gctgcgaatg
    19861 ccgttgtggc gctacgaggt cggcgtcgtc atcggcgccc tgatgtgggc cggcatctac
    19921 tccacgatcg ggttcgcggt cttcgaggcg tgggtgagcc agtcgccctg gctctggctc
    19981 ggcgccgcga taggcctcat cgccctcgtc gtagtcgcca cccgactgac caaccgacgg
    20041 ctcgagtcga cgcctcccta tgcctcgttc ccggacgtgg cggactccgg tgggagggac
    20101 tgacccaccg ctgcgcgtgg ctggctaccg ccgggtaggg tgctgacaag cattcttatc
    20161 tgcagaacga ggcacatgat gggcagccag accccggctt cggacgtgcc gggcgccgag
    20221 ttccgagccc gagcggtgcg cacgacccag gaacgacggc ccgatgtcga cccgcgagcc
    20281 atcgctctga gcctcacctt ggccaggatc gcagccaccc atcagcacca atccgagcgg
    20341 ctcgtgcacc gtgagcgggg gtggagctac agcggctttc gggtgctcta catgatctgg
    20401 ctcaccgagc cggtcgaggc acgcgacctg gcccgcttcg ccggggtgag tcgccagacc
    20461 acctcgacgg tgctctcgac cctcgaggcg gcccgcctgg tcgtgcgtcg gcagacctcc
    20521 aagaccgacc gccggctggt ctcggtgcgg ctgacgcctc ggggccgagc cgcggtcgac
    20581 gaggcgatcg gcgcccagaa cgccctcgag agtgattggt tctcggtgct cgacagtgcc
    20641 gagcaggcgc agttgcagga catgctcgaa cgcgtgttga cccgcatcga ccgcccggcc
    20701 gagactcccg ccgccgactg agcccggtca cgctgggggg cggggggagc ctggcgtacc
    20761 gcactgtgga atttccttgc ttcgctattc ctggccacac ccgcgcccag taacctgtca
    20821 aatcactgac agtccaagcg tagactcatt gtggttacca acgactgtct gcgaccgtca
    20881 tgcccgtccc tacgtccgga ggttccgtgc agcaactgac cgccctcgat gtgatgttct
    20941 cctcgctgga caccgaaacg accaatggtg tcctgggcgg gctggtgctt cacgagccgg
    21001 cagccgacgg ccgcccggcc gccgacgcgg cgttcatgcg tcaacggttc gccgagcggt
    21061 tgcccttcct gccgccgctg caccgagagg tggtcaaggt gccgatcggc atcgaccacg
    21121 actacctcgg ccagcccgac cggatcgacc tgctcgagca catccgctcc atctcgctcc
    21181 cggcgccggg cacccaggac caactggccg ccgaggtctc ccggatcatg agcacgtccc
    21241 tgccgacagg gcgcccgctg tgggactaca ccgtcatcga cgggctggcc gacggcagcg
    21301 tcgcccacct gctgcggatc caccacctgg tcatcgacgg cgggtcgatg ccgacgctgt
    21361 gggatgcgct gtccgacgag ccggccgcgc cgttggaggc atgggccgag agcacgcgcc
    21421 cgcagccgcg cttcggcaag ggcgagatgc tcgcccgaga gttgggtgga gtggtcgcca
    21481 agccggtgca gttcgccgcc ttccaggcca agttcctcgc ctgggtcgct ggccagacca
    21541 aggagaaggg acccttggcg ccgctcgccc tgccgttccg ggtcagcctg ccgggcggcc
    21601 tcgcccagcc ggcccaggcc ctgttcaact cggcgctgag caagcggggg atcgcggaga
    21661 tccagcccta catccccacc ctgcgtacgc ccacgacgcc gttcaacaac cgggtgaccg
    21721 cccaacgcac cttcgtcttc gccgacctgc cgttggcgca gttccgcgac accggcaagg
    21781 ccctcggcgg cacgatcaac gacgtcgtcc tcggagtgtg tgccggtgcc ttgcgtcgct
    21841 acctcgtgga gaacggcata cccacggacg acccgctcat cgtgtgcgtg cccgtctcga
    21901 tccgcaccgg cgccgagacg acgaagtggg ccaactacgt ccacatgatc ttcgcgccgc
    21961 tgcccacgca cctcgatgac ccgctggccc gggtgcgggc tgccggcgcc gcggtcaagg
    22021 ccgccaaggg caacttcgac gcgctgccgg tcgggttgat ccgcgaggcg agccggttca
    22081 tcccctcggc gatgttcaac gtcatgacgc gggccatggt caagatgccc gacaaggtgt
    22141 ccaagggccc gtggaacgtc gtcgtttcca acgtccgcgg acccagcacc gcggcataca
    22201 tggacggggt caagatcaag ggctactggc ccgcgtcgtt cctgtccgtc ggtggcggga
    22261 tcaacatcac cctgcagagc tacaccgacc ggatctgctt cggcttcatg ggcgcccccg
    22321 aacaggtcgg cgacctgcgg ccgctgatcg gctacatgac cgacgcgttg gccgagacgg
    22381 tcgcggctgc cggtgactcc tcggcgaagg ctgctcctgc cgaggccaag gcggcccgca
    22441 agcgcacgcc gcatcgggcc gggccaggat cggcccgact ggccgccgtc gcgccggctg
    22501 cggtcggcga cgcgacctga ccggaggccg cagatgctcg gggagccgca cgatcgcccg
    22561 cgcccggccg cgagtgactt cgtcgacacg gtcgccgacc tcactcaacg ccgggccgag
    22621 ggcgccgacc ccgagacgac ggcgctcgtc gtggcgctgt tgcgggcggg tggtcgccat
    22681 cgtggactgt tcgagcggcg cgctcacgac cagggcgggc ggcacctgcg cgcgttcagc
    22741 gtgctctacc tcgtgtggct ctacggccgg ctgagggcgc gcgacctcgc ccggctgctc
    22801 gggatgagtc gacagaccac gtccgcggtc ttggcgtcgc tcgagtcggg tggtttggta
    22861 tgccgcgagc gcggcgaggg aagggatcgg cgcctggtgg aggtccgcct caccgacgac
    22921 gggcggcgca cggtcgagcg ggagttccag gcccagcacg acctggacgg gcagtggctg
    22981 gcggtcctct caccggccga gcaacgcacc ttgcgccatc tcctggaacg cctggccacg
    23041 caacatccag gggacaacac cctggtgggt tgattgatta ccgatgcaca agggagtgct
    23101 gcatatgtcg atgcctgaca tcaccgtcgg tgcgtggttc gccgcccgcg ccgctcgttc
    23161 gccccaacgc cgggcgctca ccttcgaagg cacgagctgg acgttcgcgg agttggccga
    23221 ccggatcgaa cgcctcgccg cggtcctgag ggccggcggc gtcaagcacg gtgaccgggt
    23281 ctcgttcgtc ggcttcaacc agccggccgc gctggagacc tggttcgcct gctcgcggct
    23341 cggggcgatc tatgtgccgc tcaacttccg gttggccggc cccgagttgg ccttcatcgt
    23401 cggggacgct ggggttcaca ccctcattgc aggggcggac caacagccgg gcatcgacgc
    23461 gatccgggcc gagctgggag tccggcgtta cctcgtgacc gaggcggacg gcataccgga
    23521 ggggtgggag tcctatgagc aagcggtgac tgcggcgcac ccgttgccga cacccgaccc
    23581 ggttcgcccg gacgagttgt cgatcatcat gtacacgtcg gggaccaccg gcacccccaa
    23641 gggcgtcatg ctcagccacg agaacctctg gtggagcgac atcgccctca tgagctggat
    23701 cgacatccac gccgacgacg tgacgctggc cttctcgccg ctgttccaca tcgccgggct
    23761 caacttcatg atcggcatca cgtggatgcg cgggggagag gtcatcctgc accggtcttt
    23821 cgacgcccaa cggaccctcg aggacatcgc ccgctaccgg gtcaacaccc tcttcggggt
    23881 gcccgcgatg ttcatcgcga tcagccagtt gccgggcttc catgccgccg acctgtcgac
    23941 ggtgcggatg gctgtctgcg gcggggcgcc cgccccggag ccgctgctgc ggaccttcgc
    24001 cgatcggggg atcggcgtgc tgcaggggta tggcctcacc gagacctcgc ctgcagcgat
    24061 cttcctcgtc gccgaatacg ccctgtccaa actcggtgcc gccgggcagc cgccgctcta
    24121 catcgagacc aagctcgtgg gctcgggcgg ggtcacggtg agcgagccgg gagtgcgcgg
    24181 cgaggtgtgc atgcgcggcc cgaacgtcac ccggggttac tggaaccggc cggaggccac
    24241 ggccgccgcc ttcgacgagg ctgggtggtt ccacaccggc gatgtcggac agttcgacga
    24301 ggacggcatc atctccatcg tcgaccgggt caaggacatg gtcatcagcg gcggcgagaa
    24361 cgtctatccc gccgaggtcg agagcgtgtt gttcggccac ccgtcggttg ccgaggtcgc
    24421 cgtcatcggc ctgcccgatg cccgttgggg cgaggcggtc accgctgtcg tcgccgctaa
    24481 acccggccag agcgtcacgt tggaagacct gcgcgagtat gccgcctccc acctggcccg
    24541 cttcaagctg ccccaacggc tgcatctcgt cgaggtgttg ccacgcaatc ccacgggcaa
    24601 ggttctcaag taccagttgc gtgaggacct cggggcgaag taggccgcac gcactgagac
    24661 ccgctcgcga cgttcggtgg gtgggcaccc cgcgagtcgc caggcgggtc gtcctagact
    24721 ttcgggggca cgccgccgtg cctcggaaca ccaccccacc ggcgtcgccg tgcgccgggc
    24781 cccacgaagg aaacccccgt gaccaagccc gtggtcctca tcgccgaaga actgtcgccc
    24841 gccaccctgg atgccctcgg tcctgacttc gagatccgtt cgtgtgacgg tgccgaccga
    24901 gccgtgctgc tgccggccct ggccgacgtc gacgccgtgc tcatccgatc ggcgaccaag
    24961 atggatgccg aggcgatctc cgccgccaag cggctcaagg tcatcgcccg ggccggggtc
    25021 ggtctggaca acgtcgatgt ccccgccgcc acccaggccg gcgtgatggt cgtcaatgca
    25081 ccgacctcca acatcacctc cgccgccgag ctcgccgtgg gcctgttgct cgcctcggcc
    25141 cgcaacatcg cccccgccaa ccaggcgctt aaggccgggg cgtggaagcg cagcaagtac
    25201 ggcggcgtcg aactgctgga caagaccgtg ggcatcgtcg ggttgggtcg gatcggcgcc
    25261 ctcgtggccg agcggctcaa gggcttcggc atgcaggtgc tggcctacga cccctacacg
    25321 accccggcca aggccggcca gctcggggcc cgtctggtcg agttggacga gttgctcgcc
    25381 caggccgact tcatcacggt gcacctgccc aagaccaagg agacgctcgg cctcatcggc
    25441 gtcgacgctt tgagcaaggt caagccgacg gtgcacatcg tcaacgccgc ccgcggcggg
    25501 atcgtcgacg aagtggccct ggccgacgcc atccgcgagg gccgggtcgc cggcgccggg
    25561 atcgacgtct tcgtcaagga gccctgcacc gactcgccgc tgttcgagtt cgagcaggtc
    25621 gtcgtgacgc cccacctggg tgcctccacc gaggaagccc aggagaaggc cggtatcgcc
    25681 gtcgccaagt cggtgcgcct cgcgctcggc ggcgagatcg tccccgacgc ggtcaacgtc
    25741 tccggtggcg tcatcgtcga ggaggtccgc cctgggatcg ccctggtcga gaagctcggg
    25801 cgggtcttca cggccgtcgc cggtgcggtg ccgctgcacc tggacctcga cgtgtgcggc
    25861 gagatcaccg agcacgacgt gagcatctgg aagctcgcgg ccctcaaggg cctgttcacc
    25921 gatgtcgtcg aggaccaggt gtcctacgtc aacgcgccgg tgatcgccga ggcgcgcggc
    25981 gtcggcgtca cgctgacgac cgactcggcc agcggcgact tccgcaacgt cacgaccttg
    26041 cgcggcacgc tcaaggacgg caccgaggtg tcggtcgcgg ggacgctcac cgggccgcgg
    26101 atgatcgaga agctcgtcgg gatcaacggc ttcgacctcg aggtcccgct cagcgaccac
    26161 ctcgccgcct tcacgtatgc cgatcgccca gggattatcg gccaggtcgg cggcatcctc
    26221 ggcgactcgg cgattaacgt cggtggcatg caggtggccc gttcggagaa gcgcgacacc
    26281 gccctcatgg tgcttagcgt cgactccgag ttgccggccg aggtcgccgc ccggatcgcc
    26341 gagggcatcg gggccgtgca cttcgccgtc gtcaacctct gatctgcccg cggccgatcc
    26401 gtgccacggt cggttcgaga tctgagacgt aggtcccagc cggcaagacg gctcctaggc
    26461 tgggaccatg gtcgacacca gcgtgacaac gcctgagccc acctctctca acctcgccgt
    26521 catcggtggc gacggcatcg gccccgaggt cgtgggtgag ggtctcaagg tcctggccgc
    26581 ggtgaccggg ggagcggtgt ccatctcgac gacggactac gacctcggcg cccgccggtg
    26641 gcacgcgacc ggtgagacgc tgccggactc ggtcctggcc gagttgcgcg ggcacgacgc
    26701 gatcctgctc ggagcgatcg gcgacccgac cgtgcccagc ggtgtcctcg agcgcggcgt
    26761 gctgctgccg atccggttcg cgctcgacca ctttgtcaac ctgcgtccgg ccaagctcta
    26821 ccccggggtc gccagcccgt tggacatcgc gcgtgtcgcc cccgacggga tcgacttcgt
    26881 cgtcgttcgc gagggcaccg agggccccta tgtcggcaac ggtggcctcc tgcgcgccgg
    26941 caccccgcac gaggtggcga ccgaggtcag cgtcaacacc aggttcggcg ttgagcgggt
    27001 cgttcgcgac gcgttcgccc gcgcgcaggg ccgcccccgc aagcacctca cgctcgtgca
    27061 caagcacaac gtcctcacct tcgccggcca cctgtggcgg cgcaccgtcg aggatgtcgc
    27121 gaaggagttc ccggaggtgc agacggccta ccagcacgtc gacgccgcaa cgatcttcct
    27181 cgccaccgac ccgggtcggt tcgacgtcat cgtcaccgac aacctcttcg gcgacatcat
    27241 caccgacatc gctgcggcca tcgccggcgg gatcggcctg gccgccagcg gcaacatcaa
    27301 cccgacccgc agcaccccga gcatgttcga gccggtgcac gggtcagcgc cggacatcgc
    27361 cggccagggg aaggccgacc ccacggcagc gatcctctcg gtggcgatga tgctcgacca
    27421 cgtcggtctg gccgacgccg ctgcccgggt cgaagcggca gtggccgccg acctcgtcga
    27481 gcgcgggaac caggtgcgtt cgaccgtggc catcggcgac gcgatcgccg cccggctggc
    27541 ctgagccagc acccggccga agccgtcgtc gagctccctc gagggagcgt cagggcgacg
    27601 ccggatccgt agccgtgggc gacgactgaa ccgggtccga ggtgggagtc gcggtcggtg
    27661 ccgagggctc ggatgtcggg ctggtcggat cactcgtcag gctcggggga tcggtcgccg
    27721 tgctggtcgg gtccgaggtg ggagatggcc ccaccgtcga ggttggcacg tcggtaggac
    27781 tcgccgtcgc cgtctcggtg gggctggcgg tggcggtcgg caccacggtc tcggtcggca
    27841 ccgcagtctc ggtcggggtg agggtggggg tcaccggcgg ggtcgtcgtc aacgtcggtg
    27901 ctggcgccag gatgacatcg gagcgggcgg ggacggctgc tggaatgacc accgtgggtg
    27961 acggcgccag gtcgagatac ggcgccgggt cgacgaacgc gccgccgacc atgacctcga
    28021 agtgcagatg acatccggtt gacatgccgg tcgacccgac gtaaccgatg atctgtccct
    28081 gctggacctt caagccggca gccgcggcat acgagcgcag gtggttgtag gtggtgacca
    28141 ggccggatcc gtggtcgatg acgacccggt tgccgtaccc ggtgaacccg ccggtgagtg
    28201 tcacctggcc ggctttgacg gccttgaccg gtgtgccgca tgaggtcgcc aggtcggttc
    28261 ccgagtgcaa cttccagacg tgcaggatcg ggtggaaccg gattccgaac ggcgaggacg
    28321 ccacgccgtc gacaggcttg acgaagcccg aggcgaggac ctgtgccgcg tcgaccacgg
    28381 cggtcggcac agagccggcg tcgggcgccg cgtcgagctc ggccagggcg gcggcctggg
    28441 cggcggcggc gttcgcggcg gcggccgtca tggcgttgac cgcgttgaag tggggcacct
    28501 cggattccgg gcgcttggtc aggccggact cgccgcccgc agtcgtgctg gaggttgccg
    28561 agggccgccc ggcggccacg gtcgagccga ccgcgctgga tgacacgtcg gcgtggctcg
    28621 aggctgagaa gaggagcacc gcgatcactc cggccgtgcc gagggcacct ccggcgacga
    28681 gtgggcgcgc cgcgagcggt gccgatgcgg cataccgacc gtggttgcct cgcagcgcgc
    28741 ggaccacacg gttcacgctt cgtccctccc tcaacggcat gagcccccct ggctctgcac
    28801 caaccttagg gcccgagatt cacctacgca ggtgaaccgg gagagtgggt accctgactg
    28861 acagcataac gtttccgtcc caccgacacg ttccgaggag agccacgcag atgaccggcc
    28921 aggaccgccc agctgagctg cacttcgagc tcactccgaa cgaccacccg aggtctgacg
    28981 aggagcgcgc cgcgctgttg gccaacccgg ggttcggcaa ggtattcacc gatcacatgg
    29041 ccctggccac ctggacgacc gaggccggct ggcacgacgc gcaggtgcgc gcctacggcc
    29101 ccctctcgct catgccggcc gccgcggtcc tgcactacgc ccaggaggtc ttcgaggggc
    29161 tcaaggccta ccggcacgcg gatgggtcga tctggagctt ccgcccggag gccaacgccc
    29221 agcggctgca gcgcagttgc cggcgcctgg cgctgcccga gctggctgtc gatgacttcg
    29281 tggggtcact caaggccctc gtcgaggtgg acaaggcgtg ggtccctgcg gtcggcaccg
    29341 gcgagaccag cctctacctg cggccgttca tgttcgcctc tgaggcgttc ctcggcgtgc
    29401 gcccggccgc cgcggtgacg tatgccgtga tcgcctcgcc tgccggtgcc tacttcagcg
    29461 gcggcctcaa gccggtgacc ctctggatct cgcaggacta cgcccgcgcc ggagcggggg
    29521 gcacgggcgc agccaagtgc ggcggcaact acgcctcgag cctggccggt cagttggagg
    29581 gcatcgaaca cgggtgtgac caggcggtct tcctcgactc ggggacgcac acctacatcg
    29641 aagagctcgg cgggatgaac ctcttcttcg tcacctccga cggcaagctc gtcacgcctg
    29701 agctcaccgg gaccatcctt gaggggatca cccgcagctc catcctcgag ttggccaagg
    29761 agttgggcct ggagcccgag gagcggcgca tccccatcga ggagtggaag gacgcggccc
    29821 gcagcaagaa gatcaccgag atcttcgcct gcggcacggc agcggtcgtc acgccggtgg
    29881 gcgagttgcg ctgggagggc ggctcggtcg atcaccgcgg cgacggcccc gcgatcggtg
    29941 aggtgaccat gacgatccgc gagcggctgc tggacatcca gtacggccgc gtcgaggaca
    30001 ccaacggctg gctcactcgc ctggcgtgag ccgcctgcgc ggatccggta tgccgcccgc
    30061 tcccgtttcc gaggacgcgg tgcgaccccg tttggtcgcc tccgatctgg acggaacgct
    30121 gttgcgcccg gacggcagcg tgtcggaccg gaccaggcgg gccgtgtggg gtctggccga
    30181 ccagggcatc gcgttcgtca tcgtcaccgc ccggccgccg cgctggctgc acgagttggc
    30241 cgacctcgtc ggtccgcacg gagcggcggt ctgtgccaac ggcgctttcg tctacgacgt
    30301 cgcggcccgg acggtccggg aggtgcacgg catacccgat gggcttgtca ccgacctcgt
    30361 cggtgagttg cgcgcggccg ttccgggggt gcggttcggg gccgagcggg ccagcggggc
    30421 gatggttgag ccgggatatc cgggagccga cctcgaccgt ggggccgaag cggtccgggt
    30481 ggccccgatt caggatcgcg gcggcgagcc ggtgggcaaa ctgcttgcgg tggcgccgca
    30541 ctgggacgag gccgacttcc tcgaccgggt cgcgtcggtc gtgggcaatc gggcgcacct
    30601 ggcctactcg ggagctcacg ggttggccga gatcaccggc cccggtgtga ccaaggcggc
    30661 cgggctggcc cgctgggcgg gcgacctcgg cgtcgggtcg acggacgtct gggccttcgg
    30721 cgacatgccc aacgacctgc cgatgctgcg gtgggccggt cgctcgtatg ccgtggccaa
    30781 cgctcatccg gacgtgctcg ctgcggcgag ccaccggtgc ccggcgaact cgcaggacgg
    30841 ggtcgcctgg gcgctggaat cgctcgtcag gtgacgtcca gctgaacagt gggctgacaa
    30901 tggtcgctga ccatgccttt taggtccgtt ctgcgcagat cttgagagcg ctttccctcg
    30961 cactcgtgcc gctaaaggtc tggacgcaca ccttcttcgc tactagcatc gcctcacttc
    31021 gaccgtgtga ggagcgtcac atatggctga ccttcgattg gacgacgacg agcgcaagct
    31081 cgccgagctg ggctacaagc aggagttgtc ccgatcctgg tcgggcttct cgaacttcgc
    31141 gatctctttc tcgatcatct cgatcctcgc cgggtgcttc acgaccttcg gccaggcctg
    31201 gaacaacggc ggccccgtcg cgatctcgat cggctggccg ctcatctcga tcttcatcct
    31261 cgtcatcggc ttgtgcatga gcgagctggt gtcggcctat cccacctccg ggggcatcta
    31321 ctggtgggcg gccaaactcg gcggggccaa ggcggggtac tacaccggct ggctcaacct
    31381 catcggcctc atcgcggtcg atgcctcggt cgcctacggg tgtgcgacct tcttcgacct
    31441 caccttcagc accttcagtg agtcctgggc cgagggctac tcgctggccc gggtgttctg
    31501 gatgttcctc gtcatcctca tcctcgtggc cgtggtcaac atcttcagcg gccacctcat
    31561 ggcggtgatg aacaacgtct cggtctggtg gcacgtcttc ggggccctgg ccgtcatcct
    31621 catcctcgtc ttcctgccca agtcccacat gagcgttgcc gacgtgttca cgatgcgggt
    31681 gaacaacagc ggtggtctgg ccggcggttc gaccgacggg ttgggttact ggttctacgt
    31741 gctgccgctg ggcttcctgc tcacgcagta cacgatcacc ggcttcgacg cctcggcgca
    31801 cctgtccgag gagacccagg gggcctcgca cggtgcggca cgcggcatct ggcagtcgat
    31861 cttctactcg gcgatcggtg gctggatcct cctgttggcc ttcctcttcg ccgtccagga
    31921 cgccgaggag gtgaccgccg gaggtggcgg cgtggccgtc atcttcaacc aggccctcac
    31981 ctcgggatgg gccggcatcg tcctgctcat cgccagcatc gggcagttct tctgcacgac
    32041 cgcctgcatg acctccacca gtcggatgct cttcgcgttc agtcgcgacg gcgcggttcc
    32101 gggagcgtcg ctctgggcca aactcaactc gagcaaggtc ccggccaacg ccgttctcgc
    32161 ctccaccgtg gtcgccgcgc tcatcacgat cccggcgctc aaggaggtca acatcgggac
    32221 cgaggacgct ccgatcatcg ttccgaccgc gttctacgcc gtcgtgtcgg tcgcggtgat
    32281 cggcctctac ctcgcgttcc tcatcccgat ctacctgcgg tggcgtgccg gagacaagtt
    32341 caagcagggc ccgtggaacc tcgggacgaa gtggaagtgg atggccccga tcgcctgcct
    32401 ggagatcgtc gtcatctcgg tctacttcat cctccccttc accccggccg cctggctggg
    32461 caacgaggcc ttcgactgga agttcgtcaa ctacgcgccg atcctcaccg gcgggacgct
    32521 catcctgctc tggatcgggt ggcacgcgtc ggcgaagaag tggttcaccg gtcccaagac
    32581 gacgatcgac ctgcccgcgg gagtctcctc agccgaggag atcgccatgg agcatcacgg
    32641 caagtcggcc cacccggcgc aggacgccga cggcaagagg ccgtcgtcct gatcgtcgac
    32701 tcgactctcg cgcggctgct cgacgagatc ccctgtctct cagggcagcc gcgcgaggtg
    32761 accgaactcc cgggtggtct caccaaccac aactaccggg ttcgcacggc cactgcggac
    32821 gtcgtcgtcc gggtgtcccc acccggcacg gacctgctcg ccgtggaccg cgaggtggag
    32881 caccacaact cggtggccgc cgccgcggcg ggggtcggtg cccccgtcat cgactacctg
    32941 ccaggccgcg gcgtgctcgt cgtggggttc ctgaccgctc gcacctactc ggaggacgat
    33001 gtggctgcca acctgccgcg catcgccgcg gcgctgcgcc ggttgcacgc cggcccgccg
    33061 ttcgtcggtc ggctggacat gttcgcgctg cagcggcgtt acctcgcgct cgctcacgaa
    33121 cgagggatgt gtatgccgtc cggctacgac tcgctcgcgc cggtggtcgc gcgtgcggaa
    33181 gccgcgctcg cggcataccc ggagccgctc gtcccgtgcc acaacgatct gctggccgcg
    33241 aacttcctcg atgacggcca ggacgtgcgg atcatcgatt acgaatacag cgggctcaat
    33301 gagccggcct tcgagttggg caacatcgct caggagtcgg gtctgagcga cgccgccctc
    33361 gttggcctcg tcgaggcgta ctacccgcac gccgacgcga ggctgctcgc gcgggcgcgc
    33421 acctggcggg cgatctcggg ttatggctgg accctctggg gggtcatcca gcaccacgtc
    33481 agtgggatcg atgccgactt ctggggctgg gccacgcaca agtacgacac ggcggcgacg
    33541 ctgctgcggg actcggcctt cgtcgacacc ctctcggtcg ccgcagccgc accggccccg
    33601 acacctcggg caccggcatg agccccaccg acaccctgcc cagccgcgcc cgcgtcgtca
    33661 tcatcggagg cggcgtcatc ggagcgagcg tcgcctacca cctcaccgcc ctgggctgga
    33721 ccgacgtggt gctcctggag cagggcaccc tctcgtgcgg caccacctgg cacgcggccg
    33781 ggcttgtcgg tcaactgcgc gcctcggagg caggcacccg cctggtgcag tactcctgcg
    33841 agctctacga ccggatcgag gccgagaccg ggttgtcggc gggctaccgc cgctgcgggg
    33901 gcctcaccgt ggcgcgcacc cccgagcgga tggtcaccct gcgccggacg gcggccagcg
    33961 cagcggcata cgggctggac tgcgacttgc tcaccccgga tcaggcgggg gagcgctacc
    34021 cgctgatccg caccgacgac ctcgtcggag cgatctggct gccggctgac gggcgagcca
    34081 acccgaccga cctcacccag gccctcgcca agggtgccaa acagcgtggc gcgacgatcc
    34141 tcgagcgcac ccgggtcctg gacatccaca ccggtcccgc cacccgcggg gtgcgggtca
    34201 ccggggtgag caccgaccgc ggcgacatcg aggcggagat tgtcgtcaac tgcgccggcc
    34261 agtgggccaa ggccgtcggc gaactcgtcg gtgccaccgt gcccctgcac tcggccgagc
    34321 acttctacgt cgtcaccgag cagatcgagg gcgtccaccg cgacctgccg atcctgcgcg
    34381 accccgacgg ctatacctac gtcaaggaag aggtcggcgg gctgctcgtc ggcgggttcg
    34441 aaccggtcgc caagccgtgg gtcgcgcccg accagatccc ctacccgttt gagttccaac
    34501 tgctcgacga ggactgggaa cacttcgaga tcctcatgag cagcgcggtc caccggctcc
    34561 cggtgctggc cgagaccggg gtgcgcaagt tctacaacgg gcccgagagc ttcacgccgg
    34621 acaaccagtt cctcctcggc gaggtgcctg gggtcgccgg gttcttcgtc ggcgccggtt
    34681 tcaactcggt cggcatcgcc tcggccgggg gagcggggcg agcgctggcc gagtggatcg
    34741 tcgagggcga gccgacgagc gacctcaccg ccgtcgacat ccgccggttc gcgcccttca
    34801 acgccaacaa cgactggctg cgcgaccgcg tcggcgaggt cctcgggctg cactactcga
    34861 tcccgtggcc caaccgtgag ttggagaccg cgcggccgtt ccggcgctcn nnnnnnnnnn
    34921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggatgctcaa ctccgccggc ggctacgaag
    35041 ccgacgtcac ggtcacgcga gtggccccga cgcagttcct cgtcgtcagc ggcgcggcct
    35101 cggccgtgcg cgacgttgac tggatccgcc gcaaagcgcc cgacggccac cgcaccgagg
    35161 tcgtcgacgt caccgcgatg tatgccgtgt tcggcgtgat ggggcccgcc tcacgggagt
    35221 tgctcacgcg gctctccggc gccgacctca ccgatgcggc cttcccgttc gccaccagcc
    35281 gcgaactgcg acttggctat gcgaccgccc gcgctacccg gatcacctat gtcggcgagt
    35341 tggggtggga gctctatgtc cccgtcgagt tcgctgccgg cgtctacgac gaattgttca
    35401 acgcgggcca tttactcggt gccgctcccg cgggctacta cacgattgac gcaatgcgcc
    35461 tggagaaggg ctaccgcgcg ttcggccgcg agctcgtgcc ggatgccacc ccgatcgagg
    35521 ctgggctgag cttcacctgc aagctcgcct ccgggatcga cttcctcggt cgatcggcgg
    35581 tcgagcgggt caagaccgcc gggccgtcgc gtcggatcgt ctcgatcgtc gttggtgacc
    35641 ccactgccga acccctggca ggcgaccccg cggcatacct ctggggtggg gagttgctcc
    35701 tgcgcgacgg cgagccggtc ggccaggtga ccagcgccgg atgggggcat gccctgggag
    35761 cccccgtggg gctggcctgg gcgggctcgc ggcagactgg gccggtgact cccgaatggt
    35821 tgcgcaccgg aggatacgag gtggacgtcg ccggtcgacg cgtgccggtg accgtctcgc
    35881 tgcgtccccc cttcgacccc gacggggtcc ggctgggcag ggggccggcg tgatcatccc
    35941 cctgcacaac gacctgtccg agcatgtcct gcggcccagt tccggcaacg ccttcgagct
    36001 caccgtcgag cagttggcga ccgcgatccg cctcggcgcg ttccgcgacg gcgacctgct
    36061 cccacccgaa cgcgagctcg ccgaacgcct tgctgtgtcg cgcagcacct tgcgcgaggc
    36121 gatcgccgcc ctgcgcgact cgggcttggt gaccacgcgc cgcggccgcg gtggcggcac
    36181 catggtgacg tatgccggtg aggcgccggg gtcggtcccc ttgccccagc cgcagcgcac
    36241 cgcaaccgcg atcgccgacg tcttggactt ccgccgggtc gtcgagccgg gggcggccgc
    36301 gctggccgcg acgcgaaccc tcgccgccga tcagcgggcc tggctgctgg cctgcgccgc
    36361 ccaggtgcgc gaggcccccg attcagccac tcaccgactc aacgactccc ggctgcacct
    36421 ggccctggcc accctctcga gttcgccgat gctcgtcgag gccgtgaccc gcgcccaggc
    36481 cgccttgcat gagttgctgc gcggcatacc cgtcatctcg cgcaacatcg cccactccca
    36541 cgaccagcac gacgccatcg tcgcggctgt gctcgctggc gaccccgaga ccgcccgcgc
    36601 ggtcatggag gaacactgcg acgccacgtc gctgctgctc cgtgccctgt tgcgtcctcg
    36661 cccatgaccc gtcccccctc accgaaggat cgacgatgac ccgacccgag ctcaccctcg
    36721 accaattgcg tgccgagatc gccgacggga ccatcgacac cgtcgtggtc gccttcaccg
    36781 acatgcaagg ccgattgcag ggcaagcgga tccacggtca gtggttcgtc gacaccgtcc
    36841 tgggccacgg caccgagggc tgcaactacc tgctggccgt cgacgtcgag atgaacaccg
    36901 ttgatggcta tgccatcagc tcgtgggaga ccggctacgg cgacatgatg ttcgacctcg
    36961 acctcacgac gttgcgacgg accccggggc agaactattc ggcgacggtc cagtgcgacc
    37021 tcgcctggct cgacggcagg gggctggtgc gccagtcgcc gcgctcggtg ctgcgcgcgc
    37081 aggtggatgc tgccgcggcg ctgggtttca ccgccctcgc cggcaccgag ttggagttca
    37141 tcgtcttcga gcagtcgtat gccgccgcgt gggacgcgcg ctacgtcggc ctcacggggg
    37201 tcaaccgcta taacgtcgac tactccatcc agggcggcac cgtcgtcgaa ccgctgctgc
    37261 gcgagatccg caacgagatg tatgccgcgg gcctcaaggt cgagtcggcc aagggggagt
    37321 gcaacctcgg ccagcatgag atcgccttcc gttacgagga tgtcctgcgc acctgcgaca
    37381 accacgtcat ctacaagaac gccgccaagg agatcgccgc gaagcacggg caatcgctga
    37441 ccttcatggc gaagtacgac gaacgcgagg gcaactcctg ccacatccac ctgtcgctgc
    37501 gcggcaccga cggcagcacc gtcttcgccg acgatgggcg cgagggtggg cacagcgacg
    37561 tcttcgacca cttcctcgcc ggtgtccagg ccaccctgcg tgagctgacc tacttctacg
    37621 caccaaacat caactcctac aagcgattcc aacccggcag cttcgccccg accgcgatcg
    37681 cctggggccg ggacaaccgg acctgtgcgc tgcgcgtcgt cggtcacggc tctggcctgc
    37741 gggtggagaa ccgggtgccc ggcggcgatg tcaatcccta cctcgcggtg gcggcgatgc
    37801 tcgcgggcgg gttgcacggc atacgcgagg ggttggcgtt ggagccgcgg ttcgacggca
    37861 acgcctacgc ctccgacaag gcccgcgtgc cgaccacgtt ggtggaggcc cgcgagttgc
    37921 tcctaggctc ggccgtggcg cgggcggcgt tcggggacga ggttgtcgag cactacgccc
    37981 acgcggccca ggtggaggtc gacgccttca accgggccgt caccgactgg gaactcatcc
    38041 gcggattcga aaggctgtga gcaccacgtg aacaccagac tgagcacgac ggtgtcgagc
    38101 acgcacgagg tcatcaaccc gtcgaccgaa caggtcgtcg cgaccgtccc gtcgctgtcg
    38161 gtcgaggaga ccgatgccgc gatcgcgcgg gccgaggccg ccttcccggc gtggcgggcc
    38221 gtcagcccgg ccgatcgtgg ccgactgctg cggcggttct ccgaggtggt cgacgcccac
    38281 atcgacgaac tcgcccaact cgaggtcacc ggctctgggc acaccatcgg caacgcccgc
    38341 tgggaggccg gcaacgtccg cgacgtgctc gcctactacg cggccgcacc cgagcggttg
    38401 ttcggcaagc agatccccgt cgccggcggt ctggacgtca ccttcaagga gcccctcggc
    38461 gtcgtctcgg tgatcgtccc ctggaacttc cccatgccga tcgccggctg gggcttcgct
    38521 cctgcgctcg ccgccggcaa cgtcgtcgtc ctcaagcccg ccgagctcac cccgctcacg
    38581 gccatccgcc tgggggagtt ggccctggag gcggggctgc ccgaaggggt gttcaccgtc
    38641 ctgcccggca agggatcgat tgtcggcgaa cggttcgtca cccacccggc cgtgcgcaag
    38701 gtctgtttca ccggctcgac cgaggtcggc cagcggatca tgcgtggcgc ggccgagcag
    38761 atcaagcggg tcacccttga gttgggtggc aagagcgcca acatcgtctt cgccgactcc
    38821 gacctcgaga aggcagcggc cagcgcaccc atgtcggtct tcgacaactc ggggcaggac
    38881 tgttgcgcgc gcagccggat cctcgtgcag gccagcgtct atgaccggtt catggagttg
    38941 ttcgagaccg cagtcaaggg ggtcgtcgtc accgacccgg ccgacgaagc cgcccagatg
    39001 ggtccgctga tcagcgccga ccaccgcgcg cacgtccgcg gcttcgtcga gggcagcgcc
    39061 gactcggtcg atgtcgcctt ccgcggcacc gccccgacgg gggccggctg gtggtatccg
    39121 ccgaccgtcg tcattccccg ctcgacgcag gacacggtct ggcaggagga gatcttcggc
    39181 ccggtggttg ccgtgttgcc cttcgacgac gaagccgacg cgattgccaa ggccaacgac
    39241 acggcatacg ggctgtcggg gtcgatctgg acccgcgacg tggggcgcgc gatccgcgtc
    39301 gcccgtgggg tcgaggccgg caacctgtcg gtcaacagcc acagcagcgt ccgctatgcc
    39361 accccgttcg gcgggttcaa gcagtccggg ctcgggcgcg agctcggccc ggacgccctc
    39421 gacgcattca ccgaggtgaa gaacgtcttc ttcgccaccg acgactgacc ggatcgagcg
    39481 ctcgcgccag tcggttcacg accccgaagg aaggacacac atgtctggca ggttggacgg
    39541 caaggtcgcc gtcatcaccg gaggctgctc gggcatcggc ctggcgaccg tgcggcgatt
    39601 cgtcgccgag ggtgccaagg tcgtggtcgg cgacctcaac gacgaggccg gtgcagcgtt
    39661 ggccgacgag ctcggcatcc tctatgtccg ctgcgacgtg acgagcaaag aggccgtcga
    39721 cgcgatgttc gcggctgcca aggagcactt cggcagcgtc gacatcgcct tcaacaacgc
    39781 cggcatctcg ccgccggacg acgactcgat cctcgacacc gatctcgacg cgtggcgccg
    39841 ggtgcaggag gtcaacctca cctccgtcta cctgtgctgc aaggcggcgc tgccctacat
    39901 gatcgagcag ggcaagggct cgatcatcaa cacggcgtcc ttcgtcgcgg tcatgggggc
    39961 ggccacctcg cagatctcct actccgcctc caagggcggt gtgctgacga tgacccgtga
    40021 gttgggcgtg cagttcgccc ggcagggcat ccgggtcaac gcgctgtgcc cgggcccggt
    40081 caacaccccg ttgctgcagg agttgttcgc caaggacgcc gagcgcgctg cccggcgctt
    40141 ggtgcacatc cccatgggcc ggttcggtga gcccgaggag atggccagcg cagtgctgtt
    40201 cctcgcctcc gacgactcca gcttcatgac cgcctcgacg ttcttggtcg acggtggcat
    40261 ctcggccgct tacgtcacgc ccctctgaag cgactgagta ggcgatcgaa ccatgggtca
    40321 gccgcgccgc ccggtcattg ggatcacctg ttacgtcgag tcgatcgacc gaccgccgtg
    40381 ggtgtcccaa cccagtgccg tgctgccccg catgtatctc gagcacgtcg cccgggccgg
    40441 ggggatcccc gtgatcctcc cgcccggcga cgannnnnnn nnnnnnnnnn nnnnnnnnnn
    40501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40561 nnnnnnnnnn nnnggcgtgc tgggagcgga gacggtggag gtgcccacct accaccacca
    40621 gggggtggcg acccatcccg gctacgccgc gacggcgtgg accgccgatg gcgtcgtgga
    40681 ggcgttcgag gacccgcagg cccgcttccg gctggccgtc cagtggcacc ccgaggcggc
    40741 agaggactcc tcgctgttcc gaagtctggt cgctgcggcg acgacgtggt ccgcatcgcg
    40801 agacaccttg tccggaagcg gtccggagtg acaccctgta caacgtgacc cacaccaccc
    40861 tcgccgctgc gcgcaacatg ccagctgccc tcgttattac ctgagcgcga cgagaacact
    40921 cgtcgcgcag acctcccgca cccggggggt ctttttgttg gtccaggcac cacccccgcc
    40981 ggccagcagc gacgagaacc gaccgatacg aaagtgattc catgtccacg gccagtcccg
    41041 agttgcacgt ctacgacacg accttgcgcg atggcgccca gcaggagggt ctgaaccttt
    41101 cggtcgtaga caagctcgcc attgctcgtc agcttgacga gctcggggtc ggttacatcg
    41161 aaggcggctg gccgggcgcc aaccccaagg acacggagtt cttcgcgcgg gcgcagcagg
    41221 agttggatct gcgcaacgcc actcttgcgg cgttcggcgc tacccgccga gcggggtcga
    41281 cggctgccgc cgacccgctc gtccgggccc tgcttgactc gggtgcctcg gtcgtcaccc
    41341 tggtcgccaa gtcgcatgcc cgccacgtcg agctggcgtt gcggaccact ctcgccgaga
    41401 acctcgagat gatccgcgac acggtgacct tcctgcgcgg tgaaggccgc cgggtgttcc
    41461 ttgacgctga gcacttcttc gacggttacc gactcgaccg ggcctacgcc ctggagtgcg
    41521 tccggaccgc ggccgaggcc ggcgccgagg tcgctgcgct ctgcgacacc aacggtggga
    41581 tgctgcccgc acagatcgcc gatgtcgtcc gcgacgtcct cgactccacg ggtgcgcgcg
    41641 tgggcatcca ctgccacaac gacaccggat gcgccgtcgc gaactcgatg gctgcggtgg
    41701 ctgccggggc gacccacgtc caggggacgg tcaacggcta cggcgagcgc actggcaacg
    41761 ccaacatcct caccctcgtc tccaacctgc agatcaagca gggtatgccg ttgctcgagc
    41821 cgcagcggct gcgtgaggcc acccggatct cgcacgccat cagcgaggtc accaacgtcc
    41881 cgccctacag ccgccagccg tacgtcggct cgagcgcttt cgctcacaag gccggcctgc
    41941 acgccagcgc tctcaaggtc gaccccgacc tctaccagca caccgacccc gcgctcgtcg
    42001 gcaacgcgat gcgaacgctc gtgtcggata tggccggccg cgcgagcatc gagctcaagg
    42061 ccaaggagat gggcgtcgac ctcaccgatc agtccgagct cgtcgccgcg gttctcgccg
    42121 aggtcaagga gctcgagctg cgtggttgga ccttcgacgc ggccgacgcg tcgttcgagt
    42181 tgttgctgcg caagcacctt ggccaggcgg tgacctactt cgacgtcgag tcatggcggg
    42241 tcatcaccga cgcccgggaa gccggcgagg ccgtctctga ggcgaccgtc aagctcaccg
    42301 cgggcggatc gcgccaggtg gccaccggcg agggcaacgg ccccgtcaac gccctggacc
    42361 acgcgttgcg ggtggcgctc atgggtgtct accccgagct ggcccggctc gagctcgtcg
    42421 acttccgggt gcgcatcctc gacgcctccc acggcaccga cgcgatcacc cgggtgctca
    42481 tcgagacgtc cgacgggacg acgacctggc agaccgtagg ggtggccccc aatatcgtcg
    42541 aggcctcctg gcgagccctc gtcgacggca tcacctacgg cctgagccgc cagggggtgt
    42601 cggcgcgatg agcatcgacc acgcggcccc gccgcagacg gcgcacgcca cgcggcatac
    42661 cgcgcctgcg gccggagtgg ggctggtcct gcggcccgcc cgagccgacg aagcgccgtt
    42721 gctctccgag ctcgcgttgc gcagcaaggg ctggtggggt cacagcgcgt ccttcctgcg
    42781 ggcctgtgag gccgagttga ccctctcgcc cgagcaggcg gccaaggcgg tcctcgttga
    42841 ggtcgacggg gtcgtcgctg gcttccacct gctggccccg tcgtcggtcg gcagtgatgt
    42901 cggtgagctg gacatgctct tcgtcgaccc cccgttcatc gggtcgggga tcgggcggat
    42961 cctcttcgag gacgctcggc gctcggctgc gggtcgcggg tggacccggg tgcagatcga
    43021 ggccgaccct caggcgcgcc cgttctacga gcggctcggc gccgtgcagg tcggcgagcg
    43081 gctgagccgt tcggtgcccg gccgcagctt gccgttgttc gagttgaccc tgcgaggcgg
    43141 cggcagctcg cgccccatcg gtcgcgcgac ctactggcgc tgactctcgg cgcgaccgcg
    43201 gtgggtttgc ctgggccgat ccctgggtag tgcccaggga tcggggtgtc tttaggctgc
    43261 tacggggcta cctgtaggtc gagttgggct gcagcagaag gcaatcgggc ggccccgagg
    43321 gccgcgatcg gcacgtctga cgacagcatc gagaagcgac cgggctgcgt caactcgtcc
    43381 agcagcgggg cctgcgcggc ccgctgggcc aagacctcgg cgatcccaag agacagcggt
    43441 tcgcccaagc cggtgaggcc gccgccgatg acgatgtgct cgaccccgcc gacgaggacg
    43501 agggcttgga tggccatggc gatgccaccg cagaggtggc gcaccgcctc tgcggcctgg
    43561 gcgtcgccgg ctgcggcggc ggcgaacggg tcggcaaggt gccgcgcggg gggccacatc
    43621 cgggtgagcc ccgagccgga tgcgctggtc tccagacacc cggtctggcc gcagcggcag
    43681 ggcactggcg cggccaaagg caggtgaccg atctccccgg cgatccctcc cggcccgcgg
    43741 acgatctggc cgttgatcac caaggcggcg gccagcccgg taccgatgtt gaggtagccg
    43801 aaggccgtgt ccgggtcccc gccatgcagt tggaaggcac cccagccggc ggccttcacg
    43861 tcgttgtcga tgacgacagg ccggctcaag gcgtcgctca acgctgggcc gagcggcaga
    43921 gcgtcgacgc cgaggttgag cgcgtgcgtg accaggccgg agcgcggatc gaccagaccc
    43981 ggcatacaag caccgaccga ggcgatctgg gtccagccgc ccaccgcgtc ggcgcactcg
    44041 tgggccacgg ccaacgccac ggcaacgacc ccgtcggggc cgtgcccaga gggcgctcga
    44101 cgctcggcaa cgacgctgcc gtcggggcct aacgccacga cggcagtctt ggtcccaccg
    44161 atatcgatgc ccatcaacat ggctgcacca gtgcctcccg tggcgaagtg gtgagaagtt
    44221 cgaccaacgc cgccgcgacg gacaatgacc cggagtaggt tgccacatcg acgtcgacgt
    44281 cgacgtcgcc gaacggccac ccgcactcta cgagaacgac ggggtgaccg gaggcaccgt
    44341 atgccgcggc agcggctcgg gcgggatggt cgacgccgat cccgcggccg acgaccgcca
    44401 cgcgagcgtc tgtaggaacc tgccctgggt caatggtcac cccgagtgcc gccggacccc
    44461 agttggtcgg gccgacagcc agattggcgc gggtgtcaac ctggacgatc gccactggac
    44521 cggtgccggc ccgccacgca gcgaggctgg ggctgacgga gaacgtcccg gcgatcgtgg
    44581 cgcttgccac atccgctcgg gcggtggggg agggcagtgc cccggccgcg ggctggctgt
    44641 cgccgaccgc gagtgagtgg acgtcggcca acgcggccac ccggaccgct gcatcctgta
    44701 gccgtcgagg gtccaagacg cccgcgtcga cggcggccac gatggcgtcc agcactgcgg
    44761 catacccctc gggggtggtg tccgacccga ggcagagcag atcggctccg gcggccagtg
    44821 cgcgcaccgc agcggtcggg atgccttgcg tcgcactggc gcccgccatg tcgagggcat
    44881 cggtgacgac cacgccggtg aatccgagtt cctcgcgcag gaccccctgc aggatgggct
    44941 cggagaacgt cgccggggcg ctggggtcga gtgcggggac gaggatgtgc gaggtcatca
    45001 cggttgccac gcccgcctcg atcgccgccc ggaacggcac cagctcgcgc cgatgcaggg
    45061 tgtcgaggtc ggcgaggacg accggctgat cgaggtgcga gtcggtgtga gtgtcgccgt
    45121 gaccggggaa gtgcttggcg caggcggcca cggactgggt cgccaggccc tcgacatagg
    45181 ccacggtgtg gcgagcgacg acctcggggt cggcgccgaa gctgcgcgtc ccgatgacgg
    45241 ggttgtcgtc cgccgagttg acgtcaacca caggcgcaag atccagaccg atcccgtatg
    45301 ccgcgaggtc tgcaccgatg cgggccgcgc tgcgccgcgt ggacgcgacg tcgtccagcc
    45361 gtccgaggac ggcgttgccc ggctcggggg agccgctggg gaagtgcagg cgggtgacgt
    45421 cgccgccctc ctcgtccacg gcgatgagca gggacgtcga gatgcctcgc aggcttcgac
    45481 acaggcgatg ggtctgcgct cggtcggtga tgttgctccc gtagaggcag acggtgcgca
    45541 acccaccgtc aacggcttcg tcgatccatg caggcaccgt cgtcccgaca aagccgggca
    45601 tgagggtgct cgcagcgagg gcgcgtaact gggtaggtgc cacgtcagcc cttgacggct
    45661 ccgccgacca ggccactgct cattcgggac tggaccagca ggaagaagat gatcaccggc
    45721 acggcgacaa cggtcgacgc cgccatcacc tgaccccagt cggtctcccg gctcgcgctg
    45781 gcctggacga agccgcgcag ccaaagcggc agggtgcggg attgctcgtc ggtcatgatg
    45841 accagcgcga tggtgaactc gttccacgcc tggaggaagg cgaacacgcc cgaggccacg
    45901 aggccaggag ccaacagggg gaaggtgatc cggaggaagg cttgggtgcg ggtcaggccg
    45961 tcgaccatgc ccgcctcctc aaggtcgacc ggcacgccgg cgacgaatcc gcgcagcatc
    46021 cacaccgtga acggcacgac ggcagcggtg tagaggatcg tcagaccgag gacgctgttg
    46081 agcattccga tccccgacat catcttgtac tgcgcgatga acagcccctc ggccggcagc
    46141 atctggatga gcagcagagc caagatgaac gacttgcggc cccggaatcg gaaccgactg
    46201 atcgcgagtg cgccgaggaa ggcgatgaac agcgaggcgg caacggtgcc caaggtgacc
    46261 gcgagcgaca cgccgagggc ctgcaggaac ccggggtcgc tgaacacggc ggcgaagttg
    46321 tcgaaggacc cgccgaacgg caggaacgtc ggggtcgtcg actggagggt gacgttggac
    46381 agcatcgagg agttgaccat ccagtacacc gggaacgccc agactaaggc caggagcagg
    46441 gtgaggaccg tcaacagggc gcggccgatt ctcctgaggg tcatcaggat tcctccttca
    46501 tcaggttgcg cacgtagcgc cagctgatgg cgatggtcag taacaacatg aggatgctga
    46561 cggcgctggc ggagccgaaa tcgcccttgc ccgtgccgag ttggtagatg taggtgccca
    46621 gcagatgggt ctcgttcgcc acgccgccgg cgtcctgcaa gatcttgacc tgggtgaaaa
    46681 cgcgcaggtt ccagatgaac tgcagcagca ggacgatgga caacaccgga gcgatgaggg
    46741 gcacgatgat gtgccgaagg cgctgtatgc cgctcgcgcc gtcgatcttg gcggcctcca
    46801 acacctcggg ggagacctgg gtgagtccgg cgtagatgct caaggtgacg aaggggacgc
    46861 tcatccaggt gacgatgatg gcagcgatga tgaggaacga ggtcgggtcg gctagccagt
    46921 tgtgtccctg agcgtccacg ccgaggcgga cgagggccca gttgagcagg ccgcggcgcc
    46981 agtcgaccaa ccagatccag acggtcatgg aggcgacggc aggcatggcc caggcgagca
    47041 gaagggcgac ctggagggtg agccgggcgg ccttgtgaac cgagttcatc aacccggcga
    47101 gcgcccctcc gatgagcacg gtggcgaacg agacgacgag gcagaagacc agggaacgaa
    47161 ggacgatggc ccacagcgcg gggtcgctga cgatctctac gtagttggcc agaccgaccc
    47221 agtcggccgg aacgccgaac tgctggcgca ggccgaattc cttgaagctg gtgatgatct
    47281 gccacgcgac cgggtagccg agcgcggcga tcaacacgat gatcgtcgga gtgatgagaa
    47341 tgtgagggag ccgagatcgg cgccgcacgg gcatgttccg gccttccgcg caggagcgtg
    47401 agggtgtgag ggtggtccgg gcgggtgtgg gggtctgccc cgcccggacc accgttcgct
    47461 cgatctcagt gacaatcgag cggtctgtgg atcaggcgat gctcacttga ggttgagcat
    47521 cggcgtgatc ttggcgtcgt actccttcgc cagcgcggtg aggtccgccg cgtcgcggat
    47581 cttgccgaag aactcctcca tgaccttggc cgactcaacg gcggcccagc ccggcgcggc
    47641 cggggtcagc ttggagttgc tggccgactg gatcgtcgct gtggcgaagg cgtcggtgcc
    47701 gagcgaggag acgtagtcgg cgttggccgg gcccaggccc gccccaccga gcttcttctg
    47761 gaagtcagcg ctgaagatga tgcgcatgag ttccttggcc aggtccttgt tcttgctggt
    47821 gttggagatg gcgatgttcg aaccaccggc gaagaccggg gccggcttgc cgtcgttgcc
    47881 cggcaggacg aagacgccga aggtggcgtc attccactga cgagttggtt tgccgtcctt
    47941 ggttgccagg tcaccgatcg accagtgcgc ccaacccgga gccatgatcg tggccgcggc
    48001 aagcgacgtc gtgccggtga cggccttggt gtcgtcgagg atctggtcgg tgtcgttgat
    48061 gtagagccac ggcgaggcat ccttggcgtc agctggcgcc ttggaggcgt tgcggtagat
    48121 gtcttgcaac tgggtcaggc ccttgatggt gttggggtcc gacagggtcg acacccactg
    48181 gccactttcc ttcttggcca ggtcgccgcc gttggcgaag acccacgaga tgccgttgcg
    48241 ccagtcctgg ccaccgatga agaagccgga gaagttggcg atcttgcggg ggttggcggt
    48301 ggtgatggtc ttcaccgcgg tgttgaactc gtccagggtc ttgggcgtcg cgacgcccgc
    48361 ctgggtccag acgtccttgc gctggaacag gtaccgggaa ccgaagtagt acggcagggc
    48421 gaactgcttg ccgtccacgc ttccggcctc gacgaacgag gggagcagct tggtgccgcc
    48481 gagttcgttg tacatgtcac tgatgtccag gaaggcgccg acgttggtga acgtcgggga
    48541 ctgagtgttg cccagctcga cgacgtcagg ggtgttgttg gcgtccggga gggcggtggt
    48601 caacttggtg gtgaggtcgc cccaggactg ctcctggatc ttcagcgtgg caccggtctt
    48661 ggccttgaac gtgtcggcca ggtaggtgcg caattcggcg ggggtgtcgc caccggccag
    48721 ccacagggtg atcgacttgc tggcgttggc ggccgtgttg gtcgcggtcg gggccgaccc
    48781 cgtgctgccg ccactacagg ccgtgagcag caacgtcgct gccgtcccga ccacgagggc
    48841 ggcctggagg ttcttcttca tgggtttcct ttcccggtgg gtgggcccct gtgggggccg
    48901 gttccctggt tcggtcctac tgttctttcc tcggatgaat cgccgacatc ggtgaggggt
    48961 acgacgtcag gcacgtgccg atcgggctgg ctagctcacc cccagttggt ccagcaggac
    49021 cagggcggca gcaccccgca cgacgatgtc gttggggtcc tcgaccacgc gaacccgaag
    49081 ggtcgcgttc tcgacggtga agaggcgggc gcgcagcgtg cgctcgaccg actcgaccaa
    49141 ggccggcacg atgacgtggg cgggaccaca cagcaccacg tcggccaggt cgagggctgc
    49201 gacgaccggt gccagggcga tcgccagacg ctcgcccgcc gcggtcaccg catcagggac
    49261 gtcatcggcg gggggagcgg catcagcagg gtcgggtgtc gacgcggcca gcgcctgctc
    49321 gaggcgaggt gcggacaccc acgtctccaa gcaaccgacc ttgccgcacc ggcaccggcg
    49381 tccaccgtcg gtcccgacga cgacgtgccc gatctcaccg gccgcagagt gcgcgccgcg
    49441 gacgcggcgg ccgccgacga tgagcccgca gcccacaccg cgaccgatct tgacgagcac
    49501 gagatcgtca ccgccgtcgc cgagggtgtg gtcggcatgg gccgccgcgt cggcgtcgtt
    49561 gcacagggag acgggcaggt tggtggccga ggccacgatg tcgcgcaggg gaacgtcgcg
    49621 ccagtcgagg ttgggggcgg tcttgaccac gccgtcgctg cccacgatgc cgggggtgcc
    49681 gatgccgatc cccaggatcg gcgcggtcgc cagcgccaga cactcggcga caagttccag
    49741 cacgaggtcg actgcgcgct ctccgccgag cgggcccgcg ggacaatccg gcccagccgt
    49801 gctgccgtcc ccgatcacgg agtcgtcggt caggacaggc cgttgggcac gagcgacgac
    49861 ggtgccgtcg agatccagga cggcgccgtg cagcgcgcgg tggtcggaga ggtcgaggcc
    49921 gatcatctgc aggccggtgc ggttgacgtc gacaaggatc gagggcttgc ccatccgtcc
    49981 gggggtgctc cgcccgacct cgatggcgtg gccgcggtcg aggagggcgg tgaccaactc
    50041 ggagacggtc accttggtca agcccaatga gcgagcgagg tcggcgcggc tcatcgggcc
    50101 gctggagtag agggcctgca gggcaagcga gaggttctgg cggcgcgcgt gactgggctg
    50161 cacgggcgct gacggccgga ctcgctgtgg cctcactgaa ctcatgttag ttagtaaaca
    50221 ctactaacga agtaagaaca agtcccaggg gtgagaggtg ggtctcgtag ccctggcggc
    50281 tagtcgatgg aggcgcgctc ggccaggacg ctcgcgcagg ccgacagcag gcgcatccgc
    50341 agcgcttggc cgcgcgcggc ataccggcgt tgttcgccgg cgtaggtggc cttgccctcg
    50401 ggagtctcga cggcgatcgg ctcgatgccc aggtcgcgca ggtcgtaggg ggaggcgcgc
    50461 atgtccagga cgcgcaggtc cagcgcgagc tccaaggtgt cggcgaccag ctcgctgggg
    50521 atggcgggcg cgagtttcat cgcccacttg tagaggtcca tcgttgcgtg caggcagccg
    50581 ggctggtcga gatcggcttg gctcgcccgg gtcggagtga gcgagttgag aggacgggcc
    50641 ggctcggtga agaagcggaa ggcgtcgaaa tgactgcacc gcaacgggtt cgcttcgacc
    50701 acggcgtccg ttcctgccct cccgagacgc agcggccacc gggagtggcg cacctcgtcg
    50761 gggcgctggc ggtagaccat cgcccactcg tgcagaccga aacacccgag ctggggcggc
    50821 cggtcggcgg tgcgggtgag caggtcgcgt acgaaggtca gcgtgtcgcc gcgggccgcg
    50881 aggtatgccg cgacatcgag ttccacgccg tcatcgacga tccggtagtg ccgccactgg
    50941 gcacgcgggt cagcggccgc gccagcgagc cgcacaccca gcccggggtg ccaccgccgg
    51001 atcgccgacg gcgtgaacgg gtagtaggtg aagacgaagt cctccaccgg atgaggtatg
    51061 ccgcgtgctc gcctttcgcg atgaccggca gtgagcgcgt cgacccgatc agcatgagcc
    51121 gccgcggccg cccgccactc ctgctctgcc agcacctgcg tcacggcacg gcagcctagt
    51181 cgccgtgagc ggacggcagt cgttctaga
//
LOCUS       DAORPW010000005        44161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_103_length_44161_cov_681.993153, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000005 DAORPW010000000
VERSION     DAORPW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 44161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44161
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103_length_44161_cov_681.993153"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..2333)
                     /locus_tag="PLB86_01050"
     CDS             complement(189..2333)
                     /locus_tag="PLB86_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC63754.1"
                     /translation="MDGDLEGNARAALRRLVGRADVDFHEGQLESVLALVRDRERVLV
                     VQRTGWGKSAVYFVATALLRANGAGPTIIVSPLLSLMRDQIAAATRAGIRAVTMNSAN
                     TLDWAQVRQALADDAVDVLLVSPERLNNPAFRAEQLPDLAARAGLLVVDEAHCVSDWG
                     HDFRPDYRRIGDLLRALPPGTPVLATTATANARVVADVERELGGVDERGGEEVSAAAY
                     PHREVRTIRGGLARPSLRLGVLPSMGAEGRLGWLLTHLGELPGSGIVYTLTVAAAEDL
                     ATTLAQAGHRVAAYTGRTDPAERERLEHALRDNKVKALVATSALGMGFDKPDLGFVVH
                     VGAPSSPVAYYQQIGRAGRATERADVLLMPGAEDRDIWAYFASVSMPTEERARAVIEA
                     LTAAGRPLSTAALEAIVDIRRTRLELLLKVLDVDGAVWRVSGGWLATGESWTYDAERY
                     ERVAAARRAEQQLMLDYERTGECRMAFLQRCLDDPTAADCGRCDTCTAPWYAADVADT
                     ALGAARAQLARVGVPIEPRAQWPMGMDRLGVSVKGRIAAGEAMAPGRALARLSDLGWG
                     QRLREILQQDAEVPDAVVRAVIRVLSEWDWDERPAGVVGMPSRRHPLLVGSLAAAIAQ
                     IGRLPLLGTLDLLDGGPIGEPGGNSAFRLAGVWERLGVSPDLASRVDGIPGPVLLVDD
                     LVSSRWSLTVAARALRAAGAPAVLPLVLAVDG"
     gene            complement(2416..2808)
                     /gene="rnpB"
                     /locus_tag="PLB86_01055"
     ncRNA           complement(2416..2808)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLB86_01055"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2869..3246
                     /locus_tag="PLB86_01060"
     CDS             2869..3246
                     /locus_tag="PLB86_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC63755.1"
                     /translation="MIDHFGINCSDMPTAARFYDGVLGVLGHRRVMDFGVAIGYGTEA
                     PVFWISTFDGVGPNREVHVAFTAPDAAAVREFHAKALELGAEELHAPRLWPEYHPGYF
                     GAFVRDPDGNNIEAVCHAAGPTD"
     gene            complement(3273..4826)
                     /locus_tag="PLB86_01065"
     CDS             complement(3273..4826)
                     /locus_tag="PLB86_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRC63756.1"
                     /translation="MSEQPIHSPDPVSAALALCPDEALAPLSGADQEAAALAAIVLIE
                     QLKARLDALAVSAWRGLYAGYAREAAERLVPLVEAARAGAAVRGSLPGPAALRAQAAA
                     AVRGGVVAEVMAATGLPEGECWRRLRLATAEPARSEGLIAMLAAGEADLARVARLCDE
                     ISELDAEEAGEVALRVVAPCRDGSVASHALVGRRLRRALAQHRADDPAAAAKHAERAK
                     RARRTSAELFDDGTGVVRIVGEAARVCSVMSRIDALARGLRAGGGYSEVTLDQLRSDV
                     AFELLMRGDGRVVSDRPESGPTPVGDGASIGAASLAPAAVVGTAPLIPGQRFGAAPPA
                     HVDVIVTLATLLGCDDEPGELPGFGFVSASHVRQIAHAAGSVWRRLVVDDRTGALIEV
                     STHRYRPTEAMRTHVAARDRVCRGPGCQVSPIGADLDHDVPWPAGESSTANLSVKHRR
                     HHQLKTFGLWSTRQDPETASVTWTTLAGRSYVTEPHDYLDGRHAGAWAAHAVSTRHTD
                     DPADDPPPF"
     gene            4983..5786
                     /locus_tag="PLB86_01070"
     CDS             4983..5786
                     /locus_tag="PLB86_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HRC63757.1"
                     /translation="MPPFRKSRADAPVGYFAWEAAGLRWLGAATDAGGVPVATVLQVG
                     PTHLDLVRLDPVPPTRAAAAAFGADLARTHDPGGADVGGADVGGANPAFGAGPPHWLG
                     DGFFGPTDQPLPLSLTPHFRWSAFYAAERLTPITQGCRDAGVFDATEAALLDRLAATI
                     DRHDTDEAPSRIHGDLWSGNVIWTSAGATLIDPAAHHGHREADLAMLALFGSPHRDVT
                     IAAYQDVRPLADGWRERVALHQLYPLAVHALLFGGGYAAQTISAARRYA"
     gene            complement(5828..7291)
                     /locus_tag="PLB86_01075"
     CDS             complement(5828..7291)
                     /locus_tag="PLB86_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRC63758.1"
                     /translation="MPSRVVSYRPGPPPTLAGEPGSLARRFATIREQLHVPGEFPPDV
                     LAAAAAAADLVVLPTVDLTDLGFWTLDPVGSMDLDQAMVLAREGTGYRVRYAIADVPA
                     FVTPGGAIDVEARRRGQTIYLPDTRVPLHPPALSEGAASLLPDQVRPAYVWDLGLDEH
                     ATVTSTHVGRALVRSRARRDYASVQASVDAGTADEQVRLLQEIGQKRAALESARGGAS
                     LPMPEQQVVHEGPGVYRLEFRPPLAAEDWNAQISLMTGMAAAQLMLDAGVGILRTMPA
                     PQRDSVRRFRRQARALGVPWPDGQRYGDFLRGLDRHDPRHLALIHEATSLFRGAGYTP
                     FDGGPPEQQQHAAVAAPYAHVTAPLRRLVDRFGLVVCAAVSAGEPVPTWARESLALLP
                     GLMAEGDRRASAVERACTDAVEAAVMISLVGQSLSGSVVDRNGRGVPVVQLLDPAVVA
                     LAQGTAESGDQVTVRVVGADVAAGRVDLEVVPAPASV"
     gene            7313..7930
                     /locus_tag="PLB86_01080"
     CDS             7313..7930
                     /locus_tag="PLB86_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRC63759.1"
                     /translation="MTDPPNAHLELSLVSHQPVHSPGADTIALALALTRQAPPRCGSV
                     RVVAIDGPSGSGKTTLAAALAQALDAPTVHMDDLFPGWDGLAAAPALLTTQVLEPLAR
                     GERAAYRRWDWIASAWGDVVPVPATDVLVVEGCGASVGPAARYAAVRIWVEAELDVRM
                     ARGIARDGEMYRPHWQRWADQEVTVFGSDHTRTRADIVLDTTHVP"
     gene            7941..8516
                     /locus_tag="PLB86_01085"
     CDS             7941..8516
                     /locus_tag="PLB86_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HRC63760.1"
                     /translation="MEPADMADAVSASMQARTGRTVSEWVDAVTAAGIDPLDQLAVRR
                     WLKDTHGIAQNSQWTIAFAAAEAAGWVRPSADGYTDTLYAGAKAPLRPLHDAVLAVAL
                     ALGPDTEAQGRSTYIPVVRATQFAAIAPGPGGTLRVGLRFRTQVPDDARLEPAKGFAQ
                     ATHWVHLPGASLLADAAALEPLLAAAYLQNG"
     gene            8513..8956
                     /locus_tag="PLB86_01090"
     CDS             8513..8956
                     /locus_tag="PLB86_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HRC63761.1"
                     /translation="MTPSRRRLAARVALGGALGTSARLLVDALVGSPWGTWDAGVAVA
                     NVTGAFFLGIIALYPFRRRHPHEVRAFAGTGVMGAYTTFSALNLAMVDGSPRPEAVLG
                     LLVSIGGGCVAAWLGLRVGLSARRAVEAPGEVPGSVDPRDGEEDL"
     gene            8953..9378
                     /locus_tag="PLB86_01095"
     CDS             8953..9378
                     /locus_tag="PLB86_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HRC63762.1"
                     /translation="MSIGGYAVWTWLGAVAAGSAGAVLRWASVVWLHGILSRRSGATD
                     PFPLGVLVANTVASFLAGVAVAVAPVVGPQWHLVLVGGLCGGLSTLSTLAVDTVAMWQ
                     RGERWGAVGNLGATLALGLGAAMIGAGVVGSAVAAALSR"
     gene            complement(9438..9920)
                     /locus_tag="PLB86_01100"
     CDS             complement(9438..9920)
                     /locus_tag="PLB86_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRC63763.1"
                     /translation="MSEVSHPTGRRGDDDPFAPFVPRRGRIVAISLAVAAIALFTFIG
                     VFIPATAIHDFQIFDRVLFASIGWILAALFFRYATIKAVPTREGLTVRNLFLTRTVTW
                     PQVMGVQFGQGMPWPTLELNDTDTLAVMAIQRSDGPRSLQESARLNALVTGLGEARER
                     "
     gene            complement(9949..10794)
                     /gene="hisG"
                     /locus_tag="PLB86_01105"
     CDS             complement(9949..10794)
                     /gene="hisG"
                     /locus_tag="PLB86_01105"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRC63764.1"
                     /translation="MLRIAVPNKGSLAEPAADMLRQAGYKVRRDLKELIVTDPDNEVE
                     LFYLRPRDIAVYVGSGTVDAGITGRDLLLDSGSDAVEVMALGFGKSTFRYAARAGSMS
                     DVADIAGRRVATSYPGLVQGHLAGAGVSAAVVRLDGAVETAITLGVADVIADVVETGA
                     TLRSQGLAVFGDPILTSEAVLIRRAEVSEDNGVGVLRRRLLGVVTARDYVLLDYDVPA
                     AAVEAACQITPGLESPTVSPLHDPAWVAVRAMVPRKGTNRVMDELFDLGARGILVTHI
                     AACRL"
     gene            complement(10816..11079)
                     /locus_tag="PLB86_01110"
     CDS             complement(10816..11079)
                     /locus_tag="PLB86_01110"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRC63765.1"
                     /translation="MKTFDGLFAELADKAATRPAGSGTVAQLDAGVHAIGKKIVEEAA
                     EVWMAAEHEGPERAAEEISQLLYHLQVLMLASGLTLDDVYAHL"
     gene            complement(11175..11588)
                     /locus_tag="PLB86_01115"
     CDS             complement(11175..11588)
                     /locus_tag="PLB86_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HRC63766.1"
                     /translation="MDTAPTPGSMPLAAAYSRVAVTEAAVTLLRKLTDQHGPLMFHQS
                     GGCCDGSSPMCYPDGEFILGDSDVHLGDLGIGLDRAVPMWMSASQFAYWQHTHLTVDV
                     VPGRGAGFSVEAPEGVRFLIRSRLFTDDELDAFES"
     gene            complement(11593..13116)
                     /locus_tag="PLB86_01120"
     CDS             complement(11593..13116)
                     /locus_tag="PLB86_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRC63767.1"
                     /translation="MTIFAPPGSDGSPVSVASRYDNFIGGQWVPPVKGQYFENVTPVT
                     GRVFTEIARSTAEDIELALDAAHGAAPAWGRTSAADRAVVLNKIADRIEANLADLALA
                     ETWDNGKPIRETLAADLPLAVDHFRYFAGAIRAQEGGISEIDADTIAYHFHEPLGVVG
                     QIIPWNFPLLMATWKLAPALAAGNAVVLKPAEQTPWSILKLMELIADLLPDGVLNVVN
                     GFGVEAGKPLASSSRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFFSDV
                     ADERDAFYDKALEGFAMFALNQGEVCTCPSRALIQSSIYDNFLIDAVERVGKITQGNP
                     LDTDTMIGAQASNDQLEKILSYIDIGKQEGAKVLIGGNRADLGGDLSGGYYVQPTVFE
                     GHNGMRIFQEEIFGPVVSVTRFEDEADALKIANDTLYGLGAGVWSRDGGRAFRAGRSI
                     QAGRVWTNCYHAYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNLLVSYSPNALGFF"
     gene            complement(13254..14579)
                     /locus_tag="PLB86_01125"
     CDS             complement(13254..14579)
                     /locus_tag="PLB86_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRC63768.1"
                     /translation="MATLRSGRGGLAGHRDAFLTSGDAGGGVRSVVADSWMRSSASGV
                     DPDRHLAPIRMDRPEVQTYRSAHPLAQVFPLLYDVLGRAAVDCDCVMAVGDADGTLLW
                     VTGSPTVMRRAETIHFVEGAGWDEGHAGTNAPGMALHLDAATHVRSEEHFNRLVQPWT
                     CAAAPIHDPSTQQILGLVDITGGPDVATPQSLALVRAAARMAESELARLQATGALWTP
                     AAPGRRGSGDASLGPDPSAAFALRGLGQPELIAAHGGRTHRLSRRHGDILVALADHPD
                     GLTAEQLEVLVYAGQVTSSTMRAEMTRLRSILGPEVLTSRPYRLIAGAQTDWHAVAAY
                     LAAGRLRAAMQAYRGPLLPMSQSPAVVDQREILTGQVRSALIASAEADLMVAWTRSRW
                     GADDLDMWERQAETLPAGSPLRPIAVAEAARLERELVAAPIVLGRQTRA"
     gene            complement(14730..15443)
                     /locus_tag="PLB86_01130"
     CDS             complement(14730..15443)
                     /locus_tag="PLB86_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HRC63769.1"
                     /translation="MNNGNVFQQLIAATFPVGGYQMHWLELIGILIGAASAYLGMKRW
                     VWAWPVGILANVMLFFVYLGALFGADERIPLFGQAGRQIFFIVTSLYGWWRWNESKRA
                     RGVDNAGPAITPRWATSRERIGIVVGWLIGTVIVHQAFVALSSWPAQWWFYWCDAWIF
                     VGSVLATYSMARGWNEFWLCWIAVDLIGVPLGFATGYVPTAVLYIGYGAFVIYGFTQW
                     VKATREESGSAPAESPAVR"
     regulatory      complement(15556..15686)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            15853..16452
                     /locus_tag="PLB86_01135"
     CDS             15853..16452
                     /locus_tag="PLB86_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRC63770.1"
                     /translation="MSPILIAADLLAIALLVFGMFLPRHGRRDLVAAYLGVNVGVFAV
                     AAALTASGVASAGVGLGLFGVLSIIRLRSEELTQTEIAYYFGALAIGLLAGLGADAVP
                     MIGALMALVVVTLWIGDHPRIATRSVRQTLVLDRAHGDRSALHAHVERVLGGTVHEIS
                     VVKTDLVNDTTVVSVRHTPAPLVGHAGSARPASHDEHVR"
     gene            16449..17291
                     /locus_tag="PLB86_01140"
     CDS             16449..17291
                     /locus_tag="PLB86_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRC63771.1"
                     /translation="MSTAYAPATSPIATLVDRTMDLGALAPISLDDLVTSASRLTRVD
                     RKYVVPRRLLPTLLQATAGRAKVLDIDGVRDFAYRTVYLDTLDVQCFRAAGQKRRRRA
                     KIRTRSYLDSGATFLEVKTRGPRGTTVKERIEHPDVEEAGLTAAGAAFIADRLAAAQI
                     DTLDVASLVPMLSTTYHRSTLALGPDRVTIDTVPGWTALGPARGDLDRPGLAIVETKA
                     GATPTLVDHALWRLGHRPIRISKYGSGLAAVRPDLPHLKWHRVINQHLRPYPSITIHE
                     GHQS"
     gene            17288..>17478
                     /locus_tag="PLB86_01145"
     CDS             17288..>17478
                     /locus_tag="PLB86_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63772.1"
                     /translation="MTLRRFTSGLVAAGAALALVACSGNTTSTTTAAGAGSVGTVATA
                     ATSGAVSGDLATRLADNTES"
     assembly_gap    17479..17578
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17579..19008
                     /locus_tag="PLB86_01150"
     CDS             <17579..19008
                     /locus_tag="PLB86_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HRC63773.1"
                     /translation="SGAVSGDLATRLADNTESHAQADDASYDAATAVTVTLADGASKA
                     SGDGVTISGNTVTITTKGTYVLSGSLTNGQVVVNSSVEGKVRLVLNGATITNPSGAAL
                     VVTAADEAVVVLADGTTNTLTDGTGYDTTATDSPNAALFSMADLTIGGSGTLVVHGRT
                     ADGIATKDGLVILGGTITVDAVDDGIRGKDYIDVQGGTVSVTAGQDGLKSTNETDDSV
                     GWIAVSTGSVTLTAGDDGIHAEGDLAIAGGTVTVAKSAEGIEGANIAISGGIVSITAS
                     DDGVNVSGGTSGQTQGGGGGGGMQDDGSLLGISGGTVIVNSSGDGLDSNGSTEITGGL
                     IVVSGPSANNNGALDSNGGINVTGGTLIAAGSSGMAESPDATSTQGWLAATLTQSVAA
                     GKTISIVSGDTVIASYTTLRTVSSVVFTSSAIQKGQTYDIYVGGSLAGEASGTYSAGG
                     SISGASKLSTVTAGVATRGGGMGRP"
     gene            19195..20142
                     /locus_tag="PLB86_01155"
     CDS             19195..20142
                     /locus_tag="PLB86_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRC63774.1"
                     /translation="MTTSTSPRRVGRRALIGGSLAALLTLGSASAWALDTYVIDHVEV
                     TNASAATPASTVQVVQAAAATTVTSTSYTSDTAKITVSKHVTGTGANTVTYYVADVVV
                     SDATILRSAFANDQFGTNIIATTSEIADQADAVLAINGDYYGFRTTGIVIRNGVVFRD
                     SGARQGLAIYQDGTMRLYDETTTTGAQLVKEGVWNTLSFGPGLVTDGQVISGIDEVEI
                     DTNFGNHSVQGQQPRTGIGMVSANHFVFVVVDGRSSGYSRGVTMTEFADIFTDLGAQV
                     AYNLDGGGSSTMVFNGALVNNPLGKGTERGTSDIIYVAG"
     gene            20152..21141
                     /locus_tag="PLB86_01160"
                     /pseudo
     CDS             20152..21141
                     /locus_tag="PLB86_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
     gene            complement(21174..21830)
                     /gene="rpe"
                     /locus_tag="PLB86_01165"
     CDS             complement(21174..21830)
                     /gene="rpe"
                     /locus_tag="PLB86_01165"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRC63775.1"
                     /translation="MQISPSILSADFANLEAELESVSNADWAHVDVMDNHFVPNLTLG
                     LPVVEALLRVSPLPIDCHLMISDPDRWAPAYAEAGAGSVTFHIEAAADPRGLARTLRS
                     LGSRAGMALKPATPWAPYEELLPDLDMVLVMTVEPGFGGQSFMADQLPKVRAVRESVR
                     RHGGQVWIQVDGGVSAATIEACAEAGADVFVAGSAVFGPEDAAAAVEQLRALVRRHPH
                     "
     gene            complement(21840..23324)
                     /locus_tag="PLB86_01170"
     CDS             complement(21840..23324)
                     /locus_tag="PLB86_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRC63776.1"
                     /translation="MSDERRRRGGPAQKSRQRPAERARVGDPARRAAYTVLRAVADGA
                     YANLELPTVLRAQRVSGRDAAFATELTYGTLRLQGRYDPIIAIAAGRALTAIDANVLD
                     TLRLGCHQVLGMRVPAHAAVAETVALARQVNGAGAAGFVNAVLRRVSERTPQEWLDAI
                     TPEGSDAVTRLAITQSHPTWIVKALREALLGHGRSTRETVDADLGSLLAADNEAAAMS
                     LVARPGLCTVEELQRAGATPGRWARTAATLPGGDPGALAVVRDGRAAVQDEGSQLVAL
                     ALAAVPVEGTGPQRWLDLCAGPGGKAGLLAALALEQGASLVANEVSEHRAELVRHTLR
                     PALAAGSPGQVQVRVGDGREIGELEPAAYDKVLVDAPCTGLGALRRRPEARWRRTPDD
                     LAVLTKLQRELLASALDATRPGGVVAYVTCSPHLSETVFAVRDVVKKRSDVELLDAGE
                     SLRDKDGHALAGSGSGLFAQLWPDVHGTDAMFLALLRKAAPSRS"
     gene            complement(23317..24255)
                     /gene="fmt"
                     /locus_tag="PLB86_01175"
     CDS             complement(23317..24255)
                     /gene="fmt"
                     /locus_tag="PLB86_01175"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRC63777.1"
                     /translation="MRLVFAGTPEVALPSLEALIGSSHEVVGVVTRPDAPAGRGRSLL
                     PSPVRVRAQEHGIPVLTPRTPRDPDFQQELAALAPDCCPVVAYGALVPQAALDIPRRG
                     WVNLHFSLLPAWRGAAPVQHAIRSGDEVTGATTFLLEQGLDTGPIYGVMTETIRPTDT
                     SGDLLGRLALAGAGLLVATLDGIEAGHLVAKPQPGEGISHAPKVEVEDARVDWGLPAL
                     AVDRLIRSCTPAPGAWTTFRGERLKLGPLGPAGEAAPTLAPGDLLVTKAAVYAGTATS
                     PVRLGLVRAHGKKEMPAADWARGVRIESGERLGSDV"
     gene            complement(24261..24803)
                     /gene="def"
                     /locus_tag="PLB86_01180"
     CDS             complement(24261..24803)
                     /gene="def"
                     /locus_tag="PLB86_01180"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRC63778.1"
                     /translation="MSVKEIRLFGDPILRTPAAAVVDFDRELSQLVKDLTDTMMDAPG
                     AGLAAPQIGVGLRVFTYFVDGVVGHLVNPSLELSVEMQDGEEGCLSFPGMKYPTPRSL
                     RVVATGQDEHGEPVLLEGSELLARAVQHETDHLDGILFIDRMDPAQRKLAMKAIRESD
                     WVGGPPIVRISPHNTFGLGV"
     gene            complement(24883..26130)
                     /locus_tag="PLB86_01185"
     CDS             complement(24883..26130)
                     /locus_tag="PLB86_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63779.1"
                     /translation="MKLTEAVGALRERRFAWYFAARTTTSIGSAMTSVALAFAVLHIS
                     DDPGALAQVLAAHMAAMVIFLLLGGVVADRYSRTVIMQTSHALTFLTQGMAAYLVLTG
                     TAQIWHLVALEAVNGAVSAFGMPAMMGVVPLVVGPDRIQQANALLSFSRSSITIVGPA
                     LAGALVVGAGPGWALLVDSALFLVAIPMLRQVALPGRDRNVTASPSVIAELREGWGEF
                     TSRTWVWLIVVAFGVLNAVHMGAIAVLGPVIAKASPVGEQGWGLALSAEAAGTVVMTL
                     ALMRTRLRRPLVAGMLGVSLIALPMLVLGLSPRTDLLIAAFFVAGAGVEVFGVGWNTS
                     LHQHIPEAVLSRVSSYDALGSFVAMPIGAMAFGWLATVYDHRSILLVSAIGYAVLSLA
                     TLAAPSVRGLRQVGADAGPETAD"
     assembly_gap    26181..26280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26281..28146)
                     /locus_tag="PLB86_01190"
     CDS             complement(<26281..28146)
                     /locus_tag="PLB86_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483404.1"
                     /note="binding of PriA to forked DNA starts the assembly
                     of the primosome, also possesses 3'-5' helicase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosome assembly protein PriA"
                     /protein_id="HRC63780.1"
                     /translation="MPIDDAPSPLPVAAVVVDSGLAHLDRPFEYAVPPELDAAAQPGV
                     RVRVRFAGRDLDGFVVARRAKAEHPGRLTPIRRVVSPEPVLTPQIHALTRAVADRYGG
                     VLGDVLRLAIPKRHATAERALPLDPPVPSPTEPSRTEKPTRAEAGPWAAYPAGEAFLR
                     RVAAGSAPGAAWLAMPGRAPEQDWPRAFAEAAAAALSAGRGAGIVVPDHRDVVRVDEA
                     LTAVLGAGRHVRLTADQGPQARYTAWLKLLRGHVRVVVGTRTAAFAPVRDLGLVAWWD
                     DGDDLLAEPRAPYPQVRTVLAIRAEQSAAALLVGGFGRSVQVQRWVAAGRVVDLRPTP
                     ATLRAAAARVVVAGEGEVGRERDSAAARAQLPPSAWRIVHEGLTRGPVLVQVPRGGYR
                     PSLSCQVCRAPVRCTACHGPMGQDDPRHPPACTVCGTGFGIAGFRCPQCDSTAWRAAI
                     LGSRRTAEELGRAFPGVPVITSASGQVAGQVAATPALVIATPGAEPRATQGYAACLLL
                     DAWATLSRPELDAPVEALRRWLAAAALCRSALDGGVVVLAGVPDGPPFPAVEALVRWD
                     PVWLAERELTERAELGLPPAVVMAELVGTRHAVAAAVSDIVSGADLDVTTFGPLPV"
     gene            complement(28188..29423)
                     /locus_tag="PLB86_01195"
     CDS             complement(28188..29423)
                     /locus_tag="PLB86_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRC63781.1"
                     /translation="MSAEPTAAEACRLDRHASSLADLVARAVHPDGGFGWLGPDNEVV
                     LDRPVETWITCRMTHIAALEVMRRGDGAAHLRPLLDVGHDALTGRLRDHESRGWLPAV
                     GGTAPQPTDKVAYAHAFVILAAASLTAAGHPGGPALLTEALDVFDTYFWDEDAGMSRE
                     QYDRAWTRCEDYRGVNANMHTVEAMLAAGEVLGDQRYIDRALRILRRVVHEFARDADW
                     RLPEHFDAQWQVLPDYNRDRPADQFRPFGVTIGHVLEWSRLALNARHTLGEAADAPQW
                     AFLREDAAAMFQAAVARGWDVDGAEGLVYTTDFQDVPIVRERLHWVVCEGIAAAWALH
                     EVTGDPDALAWYDRLWAYADRYLLDGVRGGWRHELSPTNQPSDAIWWGKPDLYHAYQC
                     TLVPLLPGMVSFAGAVRPE"
     gene            complement(29535..30629)
                     /gene="chvE"
                     /locus_tag="PLB86_01200"
     CDS             complement(29535..30629)
                     /gene="chvE"
                     /locus_tag="PLB86_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRC63782.1"
                     /translation="MRTPIKSLLAVSAVAALALTGCGRSDSSTTATSGAAGAAAGAGT
                     LIGVALPQKTSENWVLAEQLFKDGLTAAGYKGDVQFANGGVSEQQNQIQSMVTKGAKV
                     IVVGAIDGSQLGTQLKAAKDGGATVIAYDRLIKNTAAVDYYVAYDNFKVGVLQGTALL
                     EGLKAKKPNGPYNIELFAGSPDDANAQVFFDGAMSVLTPKIADGTLKVASGQKDFTQA
                     VTPGWKAENAQKRMDTLLSANYSATDLDGVLSPNDTLARAILTSVKASGKASPVVTGQ
                     DSEVESVKSIMAGEQYSTINKDTRNLVKATIEMVTAIVKGGKPTVNDEKSYNNGVKVV
                     PANLLAPVIVTKANAKEAYANDPTLAQLVK"
     gene            complement(30710..31948)
                     /gene="gguB"
                     /locus_tag="PLB86_01205"
     CDS             complement(30710..31948)
                     /gene="gguB"
                     /locus_tag="PLB86_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRC63783.1"
                     /translation="MKKLKDIFGGDTRQFGMIFALVALIVFFQIMTNGLVLKPENVIN
                     IFNGNSYILVMAIGMVLVIIAGHIDLSVGSVAAAVGIVVALLMRDTGMHWVVAVLVGL
                     VVGAIIGAWQGFWVAYVGIPAFIVTLSGMMVFRGLNQLIGKSNTIPVGEDFQVIGAGY
                     LPEIGPNTGYNNLTVLLGLILVASVVFGAVRTRRNQVKIGAATDSATALWIRTGLISA
                     AILAVTLLMASGRKGTSFPVSGLILGALVLIYGFISTRTIIGRHVYAVGGNRHAAELS
                     GVRSKRVNFLVMMNMSILAALAGMMFVARSNASGPFDGVGWELDAIAAVFIGGAAVTG
                     GVGTVIGSIVGGLVMAVLNNGMQLLGVGADLTQIIKGLVLLIAVAFDVYNKMQGRPSI
                     IGLLMRNSKKAAAPVSDKAA"
     gene            complement(31965..33494)
                     /gene="gguA"
                     /locus_tag="PLB86_01210"
     CDS             complement(31965..33494)
                     /gene="gguA"
                     /locus_tag="PLB86_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRC63784.1"
                     /translation="MRNITKEFPGVKALENVSMVVRRGEVHAICGENGAGKSTLMKVL
                     SGVWPHGSYTGEIIYDGKPVEFKNIRQSEHAGIVIIHQELALIPELSIAENIFLGNEY
                     VKSGVIDWVETNRKARELLAHVGLNDEPQTAIKNIGVGKQQLVEIAKALNKDVRLLIL
                     DEPTAALNEDDSQHLLGLIDELRRKGITCIIISHKLNEIEQIADSITIIRDGQSVETL
                     DVKGDKVDEDRIIRGMVGREMSSRFPDYTPAIGDKFVEVRDWTVESPVIPGRMVCKGS
                     NFYVRRGEIVGFAGLMGAGRTELMRSIFGQSYGIFRSGELFLDGKPVSLRSVPAAIDA
                     GIGYVTEDRKVLGLNLLDDIKTTTVSAKLKKISKHSVVDRRAEFEAAEGYRKSLRTKT
                     PTVNEGVGKLSGGNQQKVVLAKWLFTDPELLILDEPTRGIDVGAKYEIYGIIQELAKS
                     GKAVIVVSSELPELLGLSDRIYTICEGAITGCLAKGEATQENLMKLMTTLSDRSAAAS
                     A"
     gene            complement(33719..34987)
                     /locus_tag="PLB86_01215"
     CDS             complement(33719..34987)
                     /locus_tag="PLB86_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRC63785.1"
                     /translation="MVSPLRRDEPGQPALLRSRGNVRPARQSSLREHNLSLVLRHIVD
                     AGTPMTRARLAKAVDLTRPAVSDLVDRLIAARLVVEAAPESVDRAGRPGVPLLPAPGS
                     FIGVGVDVAVDHIGVRALDLAGTLVTERIVEGDFRSSEPLAMLERVTSLTELVVAQLD
                     SLNATVAGVCLSVPGLTDPQGGIVRYAPNLGWREFDALAVLRRSARLRDLTLEVGNDA
                     NLAARAEARHRSRATGGSRLDQNFLFVSGEVGIGGAIVLGGDLATGLHGWSGEIGHSV
                     LDPAGPQCRCGATGCLEQYAGKDAILRRAHLPANTSVSRLLARARAGNSQATAALTEA
                     AEALGLASSSSLNLLDLDAVVLGGFYAQLFDVLGPLVADIINQRVLAARWVPVRVESA
                     VSGDYASLSGAALRVVDRVVAHPAPWLAPR"
     gene            complement(35058..36275)
                     /gene="metK"
                     /locus_tag="PLB86_01220"
     CDS             complement(35058..36275)
                     /gene="metK"
                     /locus_tag="PLB86_01220"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRC63786.1"
                     /translation="MSARLFTSESVTEGHPDKICDQISDAILDALLAQHPRSRVAVET
                     MVTTGLVHVAGEVTTEAYVEIPSIVRETVLAIGYDSSTKGFDGASCGVEVSIGQQSPD
                     IAQGVDTAYENRTGDGGLDVYDKQGAGDQGLMFGFACDDTAEFMPLPIHLAHRLAERL
                     TAVRKTQELPYLRPDGKTQVTIAYDGDRAVRLDTVVLSSQHAADVSIEGLLAPDIVAH
                     VIDPVLAELKDAGSDLDVSSYRALINPTGRFEVGGPMGDAGLTGRKIIVDTYGGMARH
                     GGGAFSGKDPSKVDRSAAYATRWVAKNVVAAGLARRCEVQVAYAIGAAHPVGLYVETF
                     GTETVPVDSIQRAIASVFDLRPAAIVDQLDLLRPIYQRTAAYGHFGRTDADGGFTWER
                     TDRVDALRAAVSR"
     gene            complement(36339..37634)
                     /gene="coaBC"
                     /locus_tag="PLB86_01225"
     CDS             complement(36339..37634)
                     /gene="coaBC"
                     /locus_tag="PLB86_01225"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRC63787.1"
                     /translation="MRVVLGVSGGIAAYKAASLLRRFTESGHDVTVVPTEAALQFVGA
                     PTWAALSGHPVATDVWTAVDRVPHVRIGQDADLVVVAPATADLLAKAATGQAGDLLTA
                     TLLTARCPVVFAPAMHTEMWLHPATQANVATLRSRGAHVIEPSSGRLTGADTGPGRLP
                     EPEDLYAACLAVVAAADPAVRAEAAGDDPHGDVRGDLRGVRVLVTAGGTREALDPVRY
                     LGNRSSGKQGVALAQVAAARGAEVTLVACNLSAAVGGSGSGGAIRVVPVESALDLQAA
                     VTTACADVDVVVMCAAVADFRPKHYVETKIKKTHAADGSDDSAPVIELVRNPDILAGL
                     VVGRGAAARPIIVGFAAETGDETGSVVELARVKLARKGCDLLVANEVGATKTFGEDTN
                     TVHLLFADGRPVVTVGPATKSDVAAAVWDGIQVLLPAAH"
     gene            complement(37683..37946)
                     /gene="rpoZ"
                     /locus_tag="PLB86_01230"
     CDS             complement(37683..37946)
                     /gene="rpoZ"
                     /locus_tag="PLB86_01230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRC63788.1"
                     /translation="MSGTVANPIGITNPPIDDLLAIADSKYALVIYSAKRARQINAYY
                     SQLQEGLLDYVGPLVETEVHEKPLSIALREINEGLLTAQATEG"
     gene            complement(37995..38606)
                     /gene="gmk"
                     /locus_tag="PLB86_01235"
     CDS             complement(37995..38606)
                     /gene="gmk"
                     /locus_tag="PLB86_01235"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRC63789.1"
                     /translation="MSGQDTLAEPFILPEGGAGRLVVLAGPTAVGKGTIAAYIRTHYP
                     QVWLSVSMTTRAPRPGEVDGVHYIFVADEEFDRLVAAGEFLEYAVVHGRAKYGTPRGP
                     VERALAAGRPALLEIDLQGARQVRDNLPDALLVFLAPPSWDELVNRLVGRGTETEEER
                     TVRLSTATRELAAAEEFDVVIVNDDVRRASEQLVSLMGSRPSA"
     gene            complement(38603..38920)
                     /gene="mihF"
                     /locus_tag="PLB86_01240"
     CDS             complement(38603..38920)
                     /gene="mihF"
                     /locus_tag="PLB86_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HRC63790.1"
                     /translation="MALPELTPQERAAALEKAALARRARAAVKNRLKYSQGSLAEVIA
                     EGKDDPVLGKLKVSALLESLPGVGPVRARQIMAEVGISESRRVRGLGVNQSAALIARF
                     DQS"
     gene            complement(39077..39964)
                     /gene="pyrF"
                     /locus_tag="PLB86_01245"
     CDS             complement(39077..39964)
                     /gene="pyrF"
                     /locus_tag="PLB86_01245"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRC63791.1"
                     /translation="MTTSAQAPSLLHPPSFGVLLHQAMRAYGPLCAGVDPHRGLMQAW
                     GLDYTVAGLERFAMTCAEAFAGRVAAVKPQSAFFEVFGSAGVAVLERLVADLRAAGTL
                     VILDVKRGDIGTTMDAYAEAFLGKDAPEPPDAITLSPYLGYGSLRPAIDLAHATGRGV
                     FVLALTSNPEGADVQHAVLHGQSVAGHVVAGATADNADAVARGELGSVGLVVGATVGD
                     AVDRLGLDLAGMGGPFLAPGVGAQGGTVESLRRVFGTALPHVLAASSREVLSAGPSVA
                     ALATRAQDTAAALAEALAS"
     gene            complement(39961..40920)
                     /locus_tag="PLB86_01250"
     CDS             complement(39961..40920)
                     /locus_tag="PLB86_01250"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRC63792.1"
                     /translation="MVDLSTTLAGATLPNPLMTASGCAANGKELHRFFDISRLGAFVT
                     KTVMPAPRSGRGTPRMAETPSGMLNSIGLQGPGIDAFADEDLPWLASVGARVLASIAG
                     GTPDEFAQVATRLLSSPAAASVSGIEVNISCPNVASRGLVFAIDPLASAAVIAAVRAA
                     VPAHLPVFAKLSPDVTDLVTIASACLGAGADGLTLINTTLGMAIDLDRMRPHLGGITG
                     GLSGPAIRPIAVRAIWQVRAAMFAERIPTAPIIGVGGVASGRDALELAAAGASAIQVG
                     TATFNDPEAPVRVLDELAGLLDERGYAAFADVVAAAHPERRRQ"
     gene            complement(40920..41753)
                     /locus_tag="PLB86_01255"
     CDS             complement(40920..41753)
                     /locus_tag="PLB86_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRC63793.1"
                     /translation="MRSSEPAGAYQHLVVAAPAIAALARPGQFVAATVGGATSGMLLR
                     RSFSIHAVDPDAGTVEVVVAAHAAGTTWITERRIDDVLDLVGPLGQAFPLPDGGGAAV
                     LVGGGYGSAPLFWLAQVLRQRGVHVEIVLGAASADRLFGVALAAEVADAVEVTTDDGS
                     AGTRGWVSDVLPQVLARSGATDVYACGPMAMLRAVTEAASAAGAAAHVAVEEAMACGV
                     GVCMTCVMPVIGADGRTRMVRACLDGPVFDGDRVRWDAFGDGLCRVPDDAVGAPQAGG
                     H"
     gene            complement(41813..>44161)
                     /gene="carB"
                     /locus_tag="PLB86_01260"
     CDS             complement(41813..>44161)
                     /gene="carB"
                     /locus_tag="PLB86_01260"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRC63794.1"
                     /translation="DEVPNDITKQTPASFEPTLDYVVVKVPRFAFEKFPAADPTLTTT
                     MKSVGEAMSLGRNFTEALQKALRSMERKEASFHWDGPVPDAAAVEALLAQIAVPTDGR
                     IIGVQQALRGGATVEQVYAATGIDPWFLDQIALINEVAESVRTAERLKPELLALAKRH
                     GFSDRQLASLRAMPEAVVRGVRHALGIRPVYKTVDTCAAEFAAATPYYYSSYDSETEV
                     LPRERPAVIILGSGPNRIGQGVEFDYSCVHASFALRDAGYDTVMVNCNPETVSTDYDT
                     SSRLYFEPLTLEDVLEVVHAEAAAGPIAGVVVQLGGQTPLGLAQALKDEGVLVVGTSP
                     EAINLAEDRGAFGRVLAEANLPAPRHGTAYSAQEAVAVAHEIGYPVLVRPSYVLGGRG
                     MQIVYDDATLTTYVDAATIASPEHPILVDRFLDDAIEIDVDVLYDGVDMYLGGVMEHI
                     EEAGIHSGDSACTLPPATLGAAEIERVRASSLALAHGIGVRGLMNVQFALAQDVLYVL
                     EANPRASRTVPFVAKATGVPLAKAAARIMLGSSIAALREEGLLPPTGDGGTLPAHAPM
                     SVKEAVLPFNRFRTKEGQMVDALLGPEMKSTGEVMGIDTDFGTAFAKSQLAAYGGLPT
                     SGTVFVSVANRDKRAMIFPVRALAELGFTLLATSGTADVLRRNGIKAEVVRKITERTG
                     DGPVGEGDVIEATIADRIIAGDVAMVVNTPTGQTARADGYAIRAAAIAADVTLITTVQ
                     ELGAAVQGIESMRSAPMRVTSLQEHARALNLYGITNPDGVAR"
BASE COUNT         6971 a        15619 c        15044 g         6327 t
ORIGIN      
        1 ccacccgacc gacctctgca gcctccgacg ccttcattac tcaagagtaa cttgctggac
       61 gggtcgagcc gaccggcttc gggaggcgag cgggcaggcg ggcaggcagg cgacgcccga
      121 ggtatgccgg accgccggga gcagcggtca cctcaaggag cgccgcgcac cggggagaga
      181 taccccggtc agccgtcgac ggcgaggacc agcgggagga cagcgggagc tccggcggcc
      241 cgcagggctc gcgcggcgac ggtgagggac cagcgcgagc tcaccaggtc gtcgacgagc
      301 agcaccggcc ccggtatgcc gtccacccgg ctcgcgaggt cgggtgaaac tcccagccgc
      361 tcccagaccc cagcaaggcg gaaggcactg ttgccgccgg gctcgccgat gggcccgcca
      421 tcgagcaggt cgagcgtgcc cagcaatggc aggcgaccga tctgcgcgat ggcggccgcg
      481 agcgagccga cgagcagggg gtggcggcgc gacggcatac cgacgacgcc ggccggtcgc
      541 tcgtcccagt cccattcgga cagcactcgg atgacggccc ggaccactgc atcggggacc
      601 tcggcgtcct gctggaggat ctcgcgaaga cgctgtcccc acccgagatc gctcagccgc
      661 gcgagggctc ggccgggcgc catcgcctcg cctgcggcga tccggccctt gaccgagacg
      721 cccaatcggt ccatccccat gggccattgg gcccgcggct cgatggggac gccgacccgg
      781 gccagttggg cgcgggcggc accgagggcg gtgtcggcga catccgcggc ataccagggg
      841 gcggtgcagg tgtcacagcg cccgcagtcg gccgcggtcg ggtcgtccag gcagcgttgc
      901 aggaacgcca tccgacactc gccggtccgc tcgtagtcga gcatgagttg ctgctcggcg
      961 cgacgggcgg cggcgactcg ttcatagcgt tcagcgtcgt aggtccacga ctcccccgtc
     1021 gcgagccacc caccgctgac ccgccagacc gcgccgtcga cgtcgaggac cttgagcagc
     1081 agttccaacc gagtccggcg gatgtcgacg atggcttcca gggcggcggt cgagagcggc
     1141 cgccctgcgg cagtgagggc ctcgatcacc gctcgggcgc gctcctcggt gggcatggag
     1201 acgctggcga agtaggccca gatgtcgcgg tcctccgcgc cgggcatgag cagcacgtcg
     1261 gcccgttcgg tggcccggcc cgcgcgacca atctgctggt agtaggcgac cggcgacgag
     1321 ggcgccccga cgtggacgac gaagccgagg tcgggcttgt cgaaacccat cccgagggcc
     1381 gaggtcgcga ccagtgcctt gaccttgttg tcgcgcaggg cgtgctcgag ccgttcccgc
     1441 tcggccgggt cggtgcgacc ggtgtaggcc gcgacccgat ggccggcctg ggcgagggtg
     1501 gtggccaggt cctcggcggc ggcgacggtg agcgtgtaga cgatgccgct gccgggcaac
     1561 tcccccaggt gcgtgagcag ccaaccgagc cgtccctcgg ctcccatgct cggcaggacc
     1621 cccaggcgca acgacggacg cgcgagcccg ccgcggatgg tgcgcacctc ccgatgcggg
     1681 tatgccgccg cgctgacctc ctccccgccg cgctcgtcca ccccgccgag ctcgcgttcc
     1741 acgtccgcga caacacgggc gttcgcggtg gccgtggtgg cgagcaccgg agtgcccggc
     1801 gggagggctc tcaaaaggtc gccgatgcgt cgatagtcgg ggcggaagtc gtggccccag
     1861 tcgctgacgc agtgggcctc atcgacgacg agcaggcccg cccgggcagc gaggtcgggc
     1921 aactgctccg cgcggaacgc gggattgttg agccgctcgg ggctcaccag gaggacgtcg
     1981 accgcatcgt cggccagggc ttggcgcacc tgggcccagt cgagggtgtt ggcggagttc
     2041 atcgtcaccg ctcggatgcc ggcccgggtg gccgcggcga tctggtcacg catgagggac
     2101 agcagcggcg agacgatgat cgtcggcccc gctccgttgg cccgcagcag cgccgtcgcg
     2161 acgaagtaga ccgccgactt gccccatccc gtgcgctgga cgaccagcac ccgctcgcga
     2221 tcccggacga gcgcgagaac cgactcgagt tgtccctcgt ggaagtcgac atcggcgcgt
     2281 cccacgagtc gccgaagcgc cgcccgggcg ttgccttcca gatcgccgtc cacacccgac
     2341 accgtatgcc gccccgccga caacgcccga ccctccccgc cccgcccggg atcgccgaaa
     2401 tcgtcgtcga gttggtggat gagtcggtcg gtacgccggg ttctgttccg cagcaaccgt
     2461 ttccggctgc cgcagcgacg gtcatccatc tcggccggcc attgctggcc ggctctagcg
     2521 ctctacccgc agactcgggc gggccgccct caaacgcctg ctgttcgagc ttgctccggg
     2581 tggggtttac cgagccgacc tggtcaccca ggccgctggt ggtctcttac accaccgttt
     2641 cacccttacc tcgcgagccg gcggaccggc atacgaggcg gtttgttttc tgtggcactg
     2701 tcccgcggat cactccgggt ggccgttagc caccaccctg ccctacggag cccggacgtt
     2761 cctcggcacc cggctcgcac cggatgacgc gaccgcctga ccgactcatc caccccgagt
     2821 gtatgccgct cggcagcgat gtcagaggtg cgccctagcg tgggctccat gatcgaccac
     2881 ttcggtatca actgcagcga catgcccacg gccgcacgct tctatgacgg cgtcctcggg
     2941 gtcctcggcc atcgacgagt catggacttc ggcgtggcca tcggatacgg gaccgaggcc
     3001 cccgtcttct ggatcagcac cttcgacggc gtcggcccca accgagaggt ccacgtggcg
     3061 ttcacggcac ctgatgccgc cgccgtgcgg gagttccatg ccaaagccct ggagttgggc
     3121 gccgaggagt tgcacgcacc gaggctttgg ccggaatatc accccggcta ctttggcgcc
     3181 ttcgtgcggg atcccgacgg caacaacatc gaggccgtct gccacgccgc tggccctact
     3241 gactgaggcc gttccggcgc gtccacgcgc cctcagaacg ggggcgggtc atcagcaggg
     3301 tcgtcggtat gccgggtgct caccgcatgc gctgcccagg cgccggcatg gcgcccgtcg
     3361 aggtagtcat ggggttcggt gacatagctg cgcccggcca gagtggtcca ggtgacgctc
     3421 gcggtctcgg ggtcctgacg ggtcgaccac aacccgaagg tcttgagctg gtgatgtcga
     3481 cggtgcttga ccgagaggtt ggccgtcgag ctctcaccgg ccggccaggg aacgtcgtgg
     3541 tcgagatcgg ccccgatcgg actgacctgg catccggggc cgcggcatac ccgatcgcgc
     3601 gctgcgacgt gcgtccgcat ggcctcggtc ggccggtagc ggtgagtcga cacctcgatc
     3661 agcgcacccg tgcggtcgtc aacaaccaac cgtcgccaca ccgatccggc cgcatgggcg
     3721 atctgccgga cgtgtgacgc cgagacgaac ccaaacccag gcaactcgcc cggctcgtca
     3781 tcgcacccca acaacgttgc caaggtgacg atcacgtcca catgggccgg gggcgcagcc
     3841 ccgaacctct ggcctgggat gagcggcgca gtaccgacca ccgcggcggg ggcgagggac
     3901 gcggcgccga tgctcgcgcc gtcgccaacg ggggtcggtc cactctcggg ccgatcggac
     3961 acaacccgcc cgtcgccgcg catgagcaac tcgaaagcga catcggaacg cagttggtcg
     4021 agggtcacct cggagtaacc accaccggcg cgcaacccgc gggccaaagc gtcgatccgg
     4081 ctcattaccg aacacacccg ggcggcctcg ccgacgatcc ggaccacccc ggtgccgtcg
     4141 tcgaagagct ctgccgaggt ccgacgcgcg cgcttcgcac gctcggcgtg cttggctgcg
     4201 gcagccgggt catcggcgcg gtgttgggcc agcgcgcggc gcaatctgcg ccccaccaga
     4261 gcatgcgaag cgaccgagcc atcccggcac ggagcgacca cccgcagggc gacctctcca
     4321 gcctcctcgg cgtccagctc gctgatctcg tcgcacaacc gcgcgaccct ggccaagtcc
     4381 gcctcccccg cggcgagcat cgcgatgagc ccctcggacc gggcgggctc agccgtggcc
     4441 agtcgcagcc gacgccagca ctccccctcg ggcaatccgg tggcggccat cacctcggcg
     4501 accacacccc cacgcaccgc cgcagccgcc tgcgcccgaa gcgccgccgg gccaggcagc
     4561 gatccgcgca ccgcggcacc cgcccgcgcc gcctcgacga gggggaccaa ccgctcggcc
     4621 gcctcgcgcg cataaccggc atacagcccg cgccatgccg acaccgcgag ggcatcgagc
     4681 cgcgccttca gctgttcgat gaggacgatc gctgccagag cagcggcctc ctgatcggcc
     4741 ccggacagcg gggcaagcgc ctcgtccggg cacagcgcga gcgccgccga caccgggtcg
     4801 ggcgagtgga tcggctgctc tgacatgtcc tgattctact cgaacgtctg tcccaaacca
     4861 agcccaatct atttacacac caacggtttt cgctaagagc gaacatcggg aaccgcaccc
     4921 caaaccaacc ccaagagtgg cccccaaggc acctcgcgac catcgcggca taccctcccg
     4981 gtatgccgcc cttccgcaag tcccgcgccg acgcgccggt cggctacttc gcctgggagg
     5041 ctgccgggct gcgttggctc ggcgccgcca cggatgccgg cggcgtcccc gtcgccacgg
     5101 ttctccaggt cggtccgacg cacctcgatc tcgttcggct cgatcccgtg ccacccaccc
     5161 gggccgccgc agcggccttc ggcgcagacc tcgcccgcac tcacgacccc ggcggcgcgg
     5221 acgtcggcgg cgcggacgtc ggcggcgcga accccgcctt cggcgccggc ccaccccact
     5281 ggctcggcga cggcttcttc ggccccactg atcagccgct cccgctctcc ctcactccgc
     5341 actttcgctg gtccgccttc tacgccgccg aacgcctcac ccccatcacg cagggatgcc
     5401 gcgacgcggg ggttttcgac gcgaccgagg ccgcactgct cgaccgattg gccgccacca
     5461 tcgacagaca tgacaccgac gaggcaccca gccggatcca cggcgacctc tggtcgggca
     5521 atgtcatctg gacatcggcc ggggcgacgc tcatcgaccc cgccgcccac catggtcacc
     5581 gcgaggccga cctggcgatg ctcgcgctgt tcgggtcgcc gcaccgtgac gtaacgatcg
     5641 cggcatacca ggacgtgcgg ccgctcgccg acgggtggcg tgagcgggtc gccctgcatc
     5701 agctctatcc gctggctgtc cacgccctgc tcttcggcgg cgggtatgcc gcgcagacca
     5761 tctcggctgc gcgccgctac gcctgagcct cgccccgact agaccgacgc gacgagaccg
     5821 accccggcta gaccgacgcg ggcgccggga cgacctccag gtccacccgt ccggccgcga
     5881 cgtcggcacc gaccacccgg accgtgacct gatcacccga ctcggccgtc ccctgcgcga
     5941 gcgccacgac cgccgggtcg agcaactgca cgaccggcac cccgcgaccg ttacggtcca
     6001 ccacgctgcc agacaacgac tggccgacga gcgagatcat cacggcggcc tccaccgcgt
     6061 cggtgcaggc ccgctcgacc gcggacgcgc gccggtcacc ctcggccatc aggccgggca
     6121 ggagcgcgag cgactctcgc gcccacgtcg gcaccggctc ccccgcgctc accgcggcgc
     6181 agacgaccaa cccgaaccgg tcaacgagcc ggcgcagcgg agccgtcacg tgggcatacg
     6241 gcgcagccac ggcggcgtgc tgctgctgct ccggaggccc gccgtcgaag ggcgtatagc
     6301 ccgctccccg gaagagcgag gtcgcctcgt ggatgagcgc gaggtggcgc gggtcatgac
     6361 ggtccaggcc gcgcaggaag tcgccgtagc gctggccatc cggccacggc accccgagcg
     6421 cccgcgcttg acgacggaag cgacgcaccg aatcccgttg cggcgcaggc atggtccgca
     6481 ggatccccac accggcgtcg agcatgagtt gagctgcagc cattccagtc atgagggaga
     6541 tctgggcgtt ccagtcctcg gccgccaaag gcgggcggaa ctccaagcgg tagaccccag
     6601 gcccctcgtg gacgacctgt tgctcgggca tcggcaagga ggccccgccg cgcgccgact
     6661 ccaacgccgc ccgcttctgc ccgatctcct gcagcaacct cacctgctca tccgcggtcc
     6721 cggcatcgac cgacgcctgg acgctcgcgt agtcgcgtcg ggcccgactg cgcaccaggg
     6781 cccgcccgac gtgcgtcgac gtcaccgttg cgtgctcgtc cagacccaga tcccacacgt
     6841 atgccggtcg cacctgatcc gggagcaggc tcgccgctcc ctcggacagg gccggagggt
     6901 gcagcggcac ccgggtgtcc ggcaggtaga tggtctgccc ccggcgccgg gcctccacgt
     6961 cgatcgctcc gcccggggtg acgaacgccg gcacatcggc gatcgcgtag cggacccgat
     7021 accccgtgcc ctcgcgcgcc agcaccatcg cctggtcgag gtccatcgac ccgaccgggt
     7081 cgagggtcca gaaacccagg tcggtgaggt cgaccgtcgg caggacgacc aggtcggcgg
     7141 cggcagcggc tgcagcgagc acgtccggcg ggaactcgcc aggcacatgc aactgctcgc
     7201 ggatcgtggc gaacctgcgg gccagcgacc ccggctcgcc ggcaagggtc ggcggcggac
     7261 ctgggcgata gctgacgaca cggctgggca tgggcacacc ctaggactcc tggtgaccga
     7321 cccgcccaac gcccacctag agttgtcgct cgtgtcccat cagcccgtcc actcccccgg
     7381 cgcggacacc atcgcgttgg cgttggccct gacccgtcag gcaccgccac gatgtgggtc
     7441 ggtccgggtc gtcgccatcg acgggcctag cgggtcgggc aagaccaccc tggcggccgc
     7501 gctggcgcag gccctggacg caccgacggt gcacatggac gacctcttcc cggggtggga
     7561 cggcctggcc gccgcaccgg ccctcctcac cacgcaggtc ctcgagccgc tcgcacgggg
     7621 tgagcgggcg gcataccggc gatgggactg gatcgcgagc gcatggggcg atgtcgtgcc
     7681 ggtcccggcc accgacgtgc tcgtcgtcga aggctgcggc gcgagcgtcg gcccggccgc
     7741 ccggtatgcc gcggtgcgga tctgggtcga ggcagagctc gacgtgcgca tggcccgcgg
     7801 catcgcccgt gacggcgaga tgtaccgccc gcactggcaa cggtgggccg accaagaggt
     7861 gacggtcttc gggtccgatc acacccgaac gcgagccgac atcgtcctcg acaccaccca
     7921 tgtgccctaa cgtttctcgc atggaacccg ccgacatggc cgatgccgtc tcggcctcga
     7981 tgcaggcacg caccggccgg accgtgtcgg aatgggtcga cgccgtcacc gccgcaggga
     8041 tcgaccccct cgaccaactc gccgtccgac gctggctgaa ggacacccac ggcatcgcgc
     8101 agaacagcca gtggaccatc gcgttcgcgg ctgccgaggc ggccgggtgg gtgcgaccga
     8161 gcgccgacgg ctacaccgac accctgtatg ccggtgccaa ggccccgctg cggccgttgc
     8221 acgacgcggt cctcgcggtc gccctcgcgc tcggccccga caccgaggca cagggtcgat
     8281 cgacctacat cccggtcgtc cgggccaccc agttcgccgc catcgcgccc gggcccggcg
     8341 gcaccctgcg ggtcggcctg cgcttccgca cccaggtccc cgacgacgcc cgcctcgagc
     8401 cggccaaggg tttcgcccag gccactcact gggtgcacct gccgggcgcc agcctcctgg
     8461 ccgacgccgc cgccctcgaa cccttgctcg cggcggcata cctgcagaac ggatgacccc
     8521 ctcccgccgc cgcctggccg cccgggtcgc cctcggcggc gcactcggca cgtccgcccg
     8581 gctcctcgtc gacgcactcg tcggctcgcc gtggggcacg tgggacgccg gggtcgccgt
     8641 cgccaacgtc accggggcgt tcttcctcgg aatcatcgcc ctctatccct tccgccgccg
     8701 gcacccgcac gaggtgcgtg ccttcgccgg caccggcgtc atgggtgcct acacgacgtt
     8761 ctccgccctc aacctcgcga tggtcgacgg ctcaccacgg cccgaggccg tgctcggcct
     8821 gctcgtcagc atcggcggcg ggtgcgtcgc ggcctggttg ggtctgcggg tggggctgtc
     8881 cgcgcgccgc gcggtcgagg cgcccggcga ggtccccggg tccgtcgacc cccgcgacgg
     8941 cgaggaggac ctgtgagcat cggcgggtat gccgtgtgga cctggctcgg ggcggtcgcc
     9001 gccggttcgg ccggggccgt gcttcggtgg gccagcgtcg tctggctgca cgggatcctc
     9061 tcccggcgca gcggcgccac cgatcccttc ccgcttggcg tcctcgtggc caacaccgtg
     9121 gcctcgttcc ttgccggggt ggcggtggca gtagcccccg tggtcggacc ccaatggcac
     9181 ttggtcctcg tcggcgggct ctgtggcggg ctctcaaccc tctcgaccct ggcggtcgac
     9241 acggtggcga tgtggcaacg cggcgaacgt tggggcgcgg tcggcaacct cggcgccacc
     9301 ctcgccctcg ggctcggtgc cgcgatgatc ggagctggcg tcgtcggcag cgctgtggct
     9361 gcggccctga gccgttagat cccgcgccga tagatcccgc gccaataggt ccggcgccga
     9421 tagatccagc cggacagtca gcgctcgcgg gcctctccca ggccggtcac cagggcgttg
     9481 agccgagccg actcctgcag cgaccgaggc ccatccgagc gttggatcgc catgacggcc
     9541 aaggtgtcag tgtcgttgag ttcaagggtc ggccaaggca tcccctgccc gaactgaacc
     9601 cccatgacct gcggccaggt gaccgtcctg gtgaggaaga ggttgcggac ggtgagcccc
     9661 tcgcgggtcg gcaccgcctt gatcgttgcg tagcggaaga agagggccgc gaggatccaa
     9721 ccgatcgagg cgaagagcac ccggtcgaag atctggaagt cgtggatcgc ggtcgccggg
     9781 atgaagaccc cgatgaaggt gaacagcgcg atggcggcca ccgccaggct gatcgcgacg
     9841 attcggccgc ggcgaggaac gaacggcgcg aagggatcgt cgtcgccccg acgcccggtg
     9901 ggatgactca cttcgctcat gtcgatctcc tccggaacgc cgagcgggtc acagtcgaca
     9961 ggctgcgatg tgagtgacga ggatccctcg ggcaccgagg tcgaacaact cgtccatcac
    10021 ccggttggtc cccttgcgcg gcaccatcgc ccgcacagcc acccaggccg ggtcgtgcaa
    10081 cggcgaaacg gtcggcgact ccagaccggg ggtgatctgg caggccgcct cgaccgcggc
    10141 agcgggcacg tcgtagtcga gcaggacgta gtcacgtgct gtgacgacgc cgagcaggcg
    10201 acgccgcagc accccgacgc cgttgtcctc ggatacctcg gcacggcgga tgagcaccgc
    10261 ttccgacgtg aggatggggt cgccgaagac cgccagaccc tggctgcgca aggtcgcccc
    10321 cgtctccacg acatcggcga tgacgtcggc cacgccgagg gtgatcgccg tctcgacggc
    10381 tccgtcgaga cgcacgaccg cagcgctcac gccagccccg gccaggtgcc cctgcacgag
    10441 ccccgggtag ctcgtcgcca cccgccgacc cgcgatgtcg gcgacgtcgc tcatcgaccc
    10501 cgcgcgggcg gcataccgga aggtcgactt gccgaaaccg agagccatga cctcgacggc
    10561 atcgctgccc gagtcgagca gcaggtcgcg accggtgatc ccggcgtcga ccgtgccgga
    10621 cccgacatag acggcgatgt cacgcggtcg aaggtagaag agttcgacct cgttgtcggg
    10681 gtcggtgacg atgagctcct tgaggtcccg gcggaccttg tatccggcct gccggagcat
    10741 gtcggcggcc ggctcggcca gagagccctt gttggggacg gcaatgcgca gcatgagaga
    10801 tccagaggga agcggtcaga ggtgggcgta gacgtcgtcg agggtgaggc ccgaggcgag
    10861 catcagcacc tgcaggtggt agagcagctg gctgatctcc tctgcggccc gctcggggcc
    10921 ttcgtgctcg gccgccatcc acacctcggc cgcctcctcg acgatcttct tgccgatggc
    10981 atgaacgccg gcatcgagct gcgcgaccgt gccggagccg gccggtcggg tggcggcctt
    11041 gtcggccagc tcggcgaaca gcccatcgaa cgtcttcacg aggaatcagc ctacccaggc
    11101 gcaggtatgc cgcccgccca ccttccagcg gacggacggc atacctgatg ggtctggcca
    11161 cccgccgatc gggctcagct ctcgaacgcg tcgagttcgt cgtcggtgaa cagccgggac
    11221 ctgatgagga accgcacccc ctccggcgcc tcgacggaga acccggcccc gcggccggga
    11281 acgacatcga cagtgaggtg ggtgtgctgc cagtaggcga actgactggc cgacatccac
    11341 atcggcacgg ctcggtccag cccgatgccg agatcaccca ggtggacgtc cgagtcgccg
    11401 aggatgaact cgccgtcggg gtaacacatc ggagacgagc cgtcgcagca gccaccggac
    11461 tggtggaaca tcaacggccc gtgctggtcg gtgagcttgc gcagcagggt caccgctgct
    11521 tcggtcaccg ccacgcggga gtatgccgcc gcgagcggca tactccctgg cgttggcgca
    11581 gtatccatcg gtttagaaga agccgagggc attgggcgag tatgacacca gcaggttctt
    11641 ggtctgctgg tagtggtcga gcatcatctt gtggttctcg cggccgatgc ccgactgctt
    11701 gtagccaccg aaggcggcgt gggccgggta ggcgtgatag cagttggtcc acacccggcc
    11761 ggcctggatc gagcgcccgg cgcggaaggc ccgacccccg tcgcggctcc acacgccggc
    11821 accgaggcca tagagggtgt cgttggcgat cttgagcgcg tcagcctcgt cctcgaaccg
    11881 ggtcaccgag acgaccgggc cgaagatctc ctcctggaag atgcgcatgc cgttgtgccc
    11941 ctcgaacacc gtcggctgaa cgtagtagcc gccggagagg tccccgccga ggtcggctcg
    12001 gttgccaccg atgaggacct tcgcaccctc ctgcttgccg atgtcgatgt aggacaggat
    12061 cttctcgagc tggtcgttgc tcgcctgggc gccgatcatc gtgtcggtgt ccagcgggtt
    12121 gccctgggtg atcttgccga cccgctcgac ggcgtcgatg aggaagttgt cgtagatcga
    12181 cgactggatc agcgcccgcg acgggcacgt gcatacctcg ccctggttga gcgcgaacat
    12241 cgcgaagccc tcgagtgcct tgtcgtagaa cgcatcccgc tcgtcggcaa cgtcggagaa
    12301 gaagatgttc gggctcttgc cacccagctc cagggtgacc gggatgaggt tttggctggc
    12361 gtactgcatg atcagccggc cggtcgtcgt ctcgccggtg aaggcgatct tggcgatgcg
    12421 ggacgaggac gcgaggggct tgccggcctc gacaccgaag ccgttgacga cgttgaggac
    12481 gccatcgggc agcaggtcag cgatgagttc catgagcttg aggatcgacc atggggtctg
    12541 ctcggccggc ttgaggacga ccgcgttgcc tgccgccagg gccggcgcga gcttccacgt
    12601 cgccatgagc agcgggaagt tccaggggat aatctggccg acgacgccca gcggctcgtg
    12661 gaagtggtag gcgatggtgt ctgcgtcgat ctcggagatg cccccctcct gcgcccggat
    12721 ggcaccggcg aagtagcgga agtggtcaac ggccaggggc aggtcggccg ccaaggtctc
    12781 gcggatcggt ttgccgttgt cccaggtctc ggccagcgcg aggtcggcga ggttggcctc
    12841 gatccggtcg gcgatcttgt tgaggacgac ggcgcggtcg gcggccgagg ttcggcccca
    12901 cgcgggtgcg gcgccgtgcg cggcgtccag cgccagttcg atgtcctcgg cggtgctgcg
    12961 ggcgatctcg gtgaagaccc gaccggtgac cggggtgacg ttctcgaagt attgcccctt
    13021 gaccgggggc acccactggc cgccgatgaa gttgtcgtag cggctggcga cggagaccgg
    13081 gctgccgtcg ctgccgggcg gagcgaagat cgtcattggt gcatcctccg aaggttgcgc
    13141 aggtcactct gcgactgtga cggccgtcac ggcgacggct cgtgtccgga ggctatgcag
    13201 cgcgacgttg caacaacgct gcatgcacgt tggcgaggtc tcacctgatc cggctaggcg
    13261 cgggtctggc gccccagcac gatcggtgct gcgaccaact cccgctcgag tcgggcggct
    13321 tcggccaccg cgatcggccg cagcggcgag ccagcgggca gggtttcggc ctgacgctcc
    13381 cacatgtcca ggtcgtcggc cccccagcgc gaccgggtcc aggcgaccat gagatcggcc
    13441 tcggccgagg cgatgagggc actgcggacc tgtcccgtga gaatctcgcg ctgatcgacg
    13501 actgcgggcg actgcgacat cgggagcagc ggaccgcggt aggcctgcat cgcggcgcgc
    13561 agccgcccgg ccgccaggta tgccgcgacc gcgtgccagt cggtctgggc gccagcgatg
    13621 aggcgatagg gacgcgacgt gaggacctcg ggaccgagga tcgagcgaag ccgggtcatc
    13681 tcggcgcgca tcgtcgatga ggtgacctgc ccggcataca cgagaacttc gagttgctcg
    13741 gcagtgaggc catccggatg gtcggcgagc gcgacgagga tgtcgccgtg tcgccggctc
    13801 aggcggtggg tgcgtccgcc gtgggcggcg atgagttcgg gctggcccag cccgcgcagg
    13861 gcgaaggcgg cggacgggtc ggggcccagc gaggcgtcac ccgagccgcg gcgtccggga
    13921 gcggccgggg tccacagcgc gcccgtcgcc tgcagccggg ccaactccga ctcggccatc
    13981 cgggccgcgg cgcgcaccag ggccaggctc tgcggggtgg cgacgtccgg accaccggtg
    14041 atgtccacca ggcccaagat ctgctgtgtc gacggatcgt ggatcggtgc tgccgcgcag
    14101 gtccagggct gcaccaggcg gttgaaatgc tcctctgagc gcacgtgggt ggcggcgtcc
    14161 agatgcaggg ccatgccggg ggcattggtg cctgcatggc cctcgtccca cccggcgccc
    14221 tcgacaaagt ggatggtctc ggctcggcgc atgaccgtcg gcgacccggt cacccacagc
    14281 agcgtgccgt cggcgtcccc gacggccatg acgcaatcac agtcgacggc agcccggccc
    14341 aggacgtcgt agagcaaggg gaagacctgg gcgagcgggt gggccgatcg gtaggtctgg
    14401 acctcaggtc gatccatccg gatcggggcc aagtgccggt ctgggtctac gccgctggcg
    14461 ctggagcgca tccacgagtc ggcgacgacg ctgcgaacgc caccgccagc gtccccggag
    14521 gtgaggaagg cgtcgcggtg accggccagc ccaccgcggc ccgatcgcaa tgtggccacc
    14581 gtcacagtga aacccaggac gggcgctgcc gacagcaggg cggcaccccg atgcgccgag
    14641 cggccggatc cgacctgtgg tcggatccga ccgctcgacg accttctcgt cgtctgccgg
    14701 cgatcccggt cagaagccag tgaccttgct cagcgaacgg caggcgactc ggcgggtgcc
    14761 gagccgctct cctcgcgagt ggccttgacc cactgggtga agccgtagat cacgaaggcg
    14821 ccgtaaccga tgtagaggac ggcggtcggg acgtagccgg tcgcgaagcc gagcgggacg
    14881 ccgatgaggt cgacggcgat ccagcacagc cagaactcgt tccacccacg ggccatcgag
    14941 taggtggcga gcaccgagcc gacgaagatc cacgcgtcgc accagtagaa ccaccactgc
    15001 gccggccaag aggacagcgc gacgaaggct tggtggacga tgaccgtgcc gatgagccaa
    15061 ccgacgacga tgccgatccg ctcacgactc gtcgcccacc gcggggtgat cgccggtccg
    15121 gcgttgtcga cgccacgagc ccgcttggac tcgttccacc gccaccaccc gtagaggctc
    15181 gtgacgatga agaagatctg ccgcccggcc tggccgaaca gcgggatccg ctcgtcggcg
    15241 ccgaacaggg ctccgaggta gacgaagaag agcatgacgt tcgcgaggat gccgaccggc
    15301 caggcccaga cccagcgctt catgcccaga tatgcggagg cggcaccgat gaggatgccg
    15361 atgagttcca gccagtgcat ctgatagcca ccgaccggga acgtcgcagc gatgagctgc
    15421 tgaaaaacgt tgccgttgtt catgattggt gttcgtcctt gagttcgttc tgacagggac
    15481 gaactccggg gacaccccgt accacgccgg ccccggaagc cgacacagcg gcataccggg
    15541 cgtcgtcgcg ccacgaaacg tgctgcctac catccggact ttcaccgtcg gtcctggaat
    15601 cccaccaggt caaccggccg ctggatgcgg tcgggtcgcg gactgtcacc gccggttcgg
    15661 agttacaccg accccggagc acgtgcccac agtgtatgcc gcgagcacgc cgggtccagg
    15721 cgcccgacca ccacgcggac ccggcaacgt ctcacagacg tcccacagtg acctcaccgg
    15781 atcggaacaa ctcgggacgg ctgactaagg acatcaagcc accgcgcgtc acaaccacac
    15841 acggagccca ccatgtcccc catcctcatc gccgccgacc ttctggctat cgccttgctc
    15901 gtcttcggca tgttcctgcc tcggcacggc cgacgtgatc tcgtcgccgc ctacctcggc
    15961 gtcaacgtgg gggtattcgc cgtagccgcc gccctcaccg catcgggggt ggccagcgcc
    16021 ggtgtcgggc tcggcctctt cggggtcctg tccatcatcc ggctgcggtc cgaggaactc
    16081 acccagaccg agatcgccta ttacttcggc gccctcgcga tcgggctgct ggccgggctc
    16141 ggcgccgacg ccgtcccgat gatcggcgcc ctcatggcct tggtcgtggt gacgctgtgg
    16201 atcggggacc acccgcggat cgccacccgc agcgtccgcc agacgctggt cctggaccgg
    16261 gcgcacggag atcgttcggc cctgcacgcc cacgtcgagc gcgtcctcgg cggcaccgtc
    16321 cacgagatct cggtcgtgaa gaccgacctg gtcaacgaca ccacggtggt ctcggtgcgg
    16381 catacccccg cccccctggt tggacacgcc ggcagcgccc gccctgcctc gcacgacgag
    16441 cacgtgcgat gagcacggcc tacgcacccg ccacgtcgcc catcgccacc ctcgtcgacc
    16501 ggaccatgga cctcggcgcg ctcgccccga tcagcctgga cgacctcgtc accagcgcat
    16561 cgaggctcac ccgggtggac cgcaagtacg tcgtgccccg gcggctgctt cccaccctgc
    16621 tccaagccac cgcaggtcgg gccaaggtgc tggacatcga cggggtccgc gacttcgcct
    16681 accgcacggt ctatctcgac accctcgacg tccagtgctt ccgggccgcc ggccagaagc
    16741 gccgccgccg ggccaagatc cgcacccgca gctacctcga cagcggggcc acgttcctcg
    16801 aggtcaagac ccgcgggccc cgtggcacca ccgtcaagga gcgcatcgag catcccgatg
    16861 tcgaggaggc cggcctcacc gcggccggag ccgccttcat cgccgaccga ctggccgcgg
    16921 cccagatcga cacccttgac gtggcctcgc tcgtcccgat gctgtccacg acctaccacc
    16981 gaagcaccct ggccctcggc ccggatcggg tgaccatcga cacggtgccc ggatggaccg
    17041 ccctcgggcc agctcgggga gacctcgacc ggcccggcct ggcgatcgtc gagacaaagg
    17101 ccggcgcgac ccccactctc gtcgaccacg ccctgtggcg cctcggccac cggccgatcc
    17161 ggatcagcaa gtacgggagc ggcctggccg ccgtccgccc tgacctaccc cacctcaaat
    17221 ggcatcgcgt catcaaccag cacctccgcc cgtacccgag catcaccatc cacgaaggac
    17281 atcagtcatg actctgcgcc gattcaccag cgggctcgta gcggccggag ccgccctcgc
    17341 ccttgtcgcc tgctcaggca acaccacgtc caccacgacg gctgccggcg ccgggagcgt
    17401 tggcaccgtt gccacggcag cgacctcggg cgccgtctcc ggtgacctcg ccacccgact
    17461 ggccgacaac accgaatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
    17581 tcgggcgccg tctccggtga cctcgccacc cgactggccg acaacaccga atcgcacgcc
    17641 caggccgatg acgcctccta cgacgcggcg accgccgtga cggtcactct cgccgacggc
    17701 gccagcaagg caagcggcga cggcgtgacc atcagcggca acaccgtgac gatcacgacg
    17761 aagggcacct acgtcctctc cggcagcctc accaacggcc aggtcgtcgt caacagttcg
    17821 gtcgagggca aggtgcgcct ggtcctcaac ggggcgacga tcaccaaccc gtcgggggcg
    17881 gcgctcgtgg tcaccgccgc cgacgaggcc gtcgtcgtcc tggccgacgg aaccaccaac
    17941 accctcaccg atggcaccgg ctacgacacg acggccaccg actcccccaa cgccgccctg
    18001 ttctcgatgg ccgacctcac catcggcggc agcggcaccc tcgtcgtgca cggccggacg
    18061 gccgacggca tcgccaccaa ggacggcctg gtcatcctcg gcgggacgat cacggtcgac
    18121 gccgtcgacg acggcatccg cggcaaggac tacatcgacg tccaaggcgg caccgtgagc
    18181 gtcaccgccg ggcaggacgg cctgaagtcg acgaacgaga ccgacgacag cgtcggctgg
    18241 atcgccgtct ccaccgggtc ggtcacgctc accgcgggcg acgacggcat ccacgccgaa
    18301 ggtgacctcg cgatcgccgg cgggacggtc accgtggcca agtccgccga gggcatcgaa
    18361 ggggccaaca tcgccatcag cgggggcatc gtgtcgatca ccgccagcga cgacggagtc
    18421 aacgtctccg gtggaacgtc cggccagacg cagggcggcg gcggtggcgg cgggatgcag
    18481 gacgacggct cgctgttggg gatcagcggc ggaacagtca tcgtcaactc cagtggtgac
    18541 ggactggaca gcaacggcag caccgagatc accggcggcc tcatcgtcgt gtccggaccg
    18601 agcgccaaca acaacggcgc cctggacagc aacggcggga tcaacgtgac cggtggaacg
    18661 ctgatcgccg caggcagctc cgggatggcc gagtcgcccg acgccacctc cacccagggc
    18721 tggctcgctg ccaccctcac ccagagcgtg gcggccggca agacgatctc catcgtctcg
    18781 ggcgacaccg tcatcgcgtc ctacaccacg ctgcgcaccg tctcgtcggt cgtcttcacc
    18841 tcctcggcga ttcagaaggg ccagacctac gacatctacg tcgggggctc cctggccggc
    18901 gaggcgtccg gcacctactc cgccggcggc tcgatctccg gcgccagcaa gctgagcacc
    18961 gtgaccgcag gcgtggccac ccgtgggggc gggatgggtc gcccctaacc cacctgcgcc
    19021 tccggcgccc acaccaccct ggggtatgcc gcccgctcac ctgagcgggc ggcatacctg
    19081 tgcgtgggga gtctccacgg acgaaaactg ttgtcctgca atggttcagg aacacatgac
    19141 ggacacctcg cttggtcaca gggtctgcac atgggcgact tcttgactac tcacatgacg
    19201 acctccacca gcccccgccg cgtcggccgc cgcgccctca tcggcggatc ccttgccgcc
    19261 ctcctcaccc tcggctcggc cagcgcctgg gccctggaca cctacgtcat cgaccacgtc
    19321 gaggtcacca atgcctcggc tgcgacgccg gcgagcacgg tccaggtcgt ccaggccgcc
    19381 gcggccacga cggtcacctc gacgagctac acctcggaca cggccaagat caccgtgagc
    19441 aagcacgtca ctgggacggg cgccaacacg gtcacctact acgtcgccga tgtcgtcgtc
    19501 agcgacgcga cgatcctgcg gtcggccttc gccaacgacc agttcggcac gaacatcatc
    19561 gccacgacct ccgagatcgc cgatcaggcc gacgcggtgc tggcgatcaa cggcgactac
    19621 tacggcttcc gcaccaccgg catcgtcatc cgcaacggtg tcgtcttccg cgacagcggt
    19681 gcccgccagg gcctggcgat ctaccaggac gggacgatgc gcctctacga cgagacgacg
    19741 acgaccggcg cgcaactggt caaggaaggc gtctggaaca ccctgagctt cgggccgggc
    19801 ctcgtcaccg atggccaggt catctccggg atcgacgagg tcgagatcga caccaatttc
    19861 ggcaaccact cggtccaggg ccagcagccc cgcaccggca tcggcatggt ctccgccaac
    19921 cacttcgtgt tcgtcgtcgt cgacggccgc tccagcggct actcccgtgg cgtcacgatg
    19981 accgagttcg ccgacatctt caccgacctc ggcgcgcagg tcgcctacaa cctcgacggc
    20041 ggcggctcct cgaccatggt gttcaacggc gctctggtca acaacccgtt gggcaagggc
    20101 accgagcgcg gcacctcgga catcatctac gtggccggct aggtgaggga gatgatcgtc
    20161 ctcatcccgt cctacgagcc cgacatccgg ctcgtccgcc tcgtcgagtc gctgcggatg
    20221 gtgcgcggtt tgcgagtcct cgtcgtcgac gacggcagcg gcccggcgta tgccgcccac
    20281 ttcgccgccg tcgccgccct cggcgccacg gtcctggtcc acgaggtcaa ccggggcaag
    20341 ggagccgcgc tcaagaccgg cttctcgcac gctctggcca cctggccagg agagtcgctc
    20401 gtctgcgccg actcggacgg tcagcacgcg cccgccgaca tcctgcgggt gggcgaggcg
    20461 atcgcggaag gccccgatgt cgtcctcggg gtgcgccggt tcaccggtcg ggtgccgttg
    20521 cggagccgga tcggcaacga cctcacccga cggcttttcg cgctcgcgac gggagtgccg
    20581 atcatcgaca cccagaccgg gctgcgcggt tacccgtcgc gggtcctccc ctggctgctg
    20641 gacatccccg gcgaccggtt cgactacgag ttgcggacgt tgttggccgc tgcacgcgag
    20701 cgccgaagca cggtccaggt cgagatcgag acgatctacc tcgacgacaa cgcctcgtcg
    20761 cacttccggc cggtgcgcga ctccgcgctc atctaccggc aactcgcggc cttcgccgcc
    20821 tcgtcgctgc tcggcttcac cgtcgacgtc gtcgtcctgg ccttggccat ctggctctcc
    20881 ggcaacctgg tggcgtcggt cgtcgctgcc cgcctggtca gtgcgagcgt caactacacc
    20941 gtcaaccgcc ggttcgtctt cgccgccgac ggggtcgcga gcacccgcgc ctcactgccc
    21001 cggtatgccg gcctcgcggc ggtcatcctc ggagcgaacg tcctgctcat gcagggtctc
    21061 gtcgccgtga ccgggtcgac agtcatcgcc aaggtcgtca ccgaggtgac cctgtttgtg
    21121 ctctcctacg gagtccagcg cacgctggtg ttcgccggcg gtcgccggcc aggtcagtgc
    21181 ggatgccgcc tgacgagggc gcgtagctgt tcgaccgccg cggcggcgtc ctccggcccg
    21241 aacacggccg agccggcgac gaagacgtcg gcccccgcct ccgcacacgc ctcgatggtc
    21301 gcggcgctca ccccgccgtc gacctggatc catacctgac caccatggcg gcgcaccgac
    21361 tctcggaccg cccgcacctt cggcagttgg tcggccatga acgactggcc gccgaagccc
    21421 ggctcgacgg tcatgacgag caccatgtcc agatcgggca gcaactcttc gtagggcgcc
    21481 cacggggtcg ccggcttgag cgccatcccg gcccgggacc ccaacgaccg cagggtgcga
    21541 gccagcccgc gcgggtcggc cgccgcctcg atgtggaagg tcaccgatcc ggcacccgct
    21601 tcggcatacg ccggagccca gcgatcgggg tcggagatca tcaggtgaca gtcgatcggc
    21661 agcgggctca cccgcaggag cgcctcgacg accggcagcc ccagcgtgag gttcggcacg
    21721 aagtggttgt ccatgacgtc cacgtgcgcc cagtcggcgt tgctcaccga ctcgagctcg
    21781 gcctccaggt tggcgaagtc cgcggacagg atgctcggtg agatctgcac aacccaaccc
    21841 taggaccggc tcggcgccgc cttgcgcagc agcgcgagga acatcgcgtc ggtgccgtgc
    21901 acgtctggcc agagttgggc gaacaacccg cttccggacc ccgccagcgc atgtccgtcc
    21961 ttgtcccgca acgattcccc agcgtcgagg agctcgacgt ccgagcgctt cttgaccacg
    22021 tcgcgcaccg cgaacaccgt ctccgacagg tgtgggctgc aggtgacgta ggcgaccacc
    22081 ccgccggggc gggttgcgtc cagggcactg gccagcaact cgcgctgcag tttggtgagc
    22141 accgcgaggt cgtcgggggt tcgccgccac cgcgcttctg ggcgacgacg cagggcgccg
    22201 aggcccgtgc acggcgcatc cacgaggacc ttgtcgtatg ccgcgggctc caactcgccg
    22261 atctctcggc cgtcccccac ccgcacctgc acctgccctg ggctccccgc cgcaagcgcc
    22321 ggccggaggg tatgccgcac caactcggcc cgatgttcgc tcacctcgtt ggcgaccagc
    22381 gaggcaccct gttcgagggc gagcgcggcc agcagacccg ccttgccgcc tgggccggcg
    22441 cacaggtcga gccagcgctg cgggccggtg ccctcgacgg ggaccgccgc caacgcgagc
    22501 gcgaccaact gggagccttc gtcctgcact gcggcgcgcc cgtcacgcac caccgcgaga
    22561 gcccccgggt cgccacccgg cagggttgcc gccgtgcgcg cccagcgccc cggggtcgct
    22621 ccggcgcgtt ggagctcctc gaccgtgcac agacccggcc gggcgacgag ggacatggct
    22681 gccgcctcgt tgtcggccgc cagcagactg cccaggtctg cgtcgacggt ctcccgtgtc
    22741 gaacggccat ggccgagcaa cgcctcgcgc agcgccttga cgatccacgt cgggtgcgac
    22801 tgggtgatcg cgagacgcgt caccgcgtcc gaaccctcgg gcgtgatggc gtccagccac
    22861 tcctgcgggg tccgctcgct cacccggcgc aggaccgcgt tgacgaaacc ggccgcgccg
    22921 gcgccgttga cctgcctggc taaggccacg gtctcggcga cggcggcgtg ggccgggact
    22981 cgcatcccca acacctgatg acagcccagt cgcagggtgt ccaggacgtt ggcgtcaatc
    23041 gccgtgagtg cccgcccggc cgcgatcgcg atgatcgggt cgtagcggcc ctgcagccgc
    23101 agggtcccgt aggtgagctc cgtggcgaac gcggcgtcgc gcccagacac ccgctgggcg
    23161 cgcagcaccg tggggagttc gaggttggca taggcaccgt cagcgaccgc ccgcaggacg
    23221 gtgtatgccg cgcgccgcgc cggatcgccg acccgagctc gttcggccgg ccgttgccgg
    23281 gacttctgcg ccgggccgcc ccgtcgacgt cgctcgtcag acatccgagc ccagccgctc
    23341 ccccgactcg atccgcacac cgcgcgccca atcggccgca ggcatctcct tcttgccgtg
    23401 cgcgcggacc aggccgaggc ggaccgggga ggtggcggta ccggcataca ccgctgcctt
    23461 ggtgaccaac aggtcgccgg gagccaacgt cggggcagcc tcgccggcgg gcccgagcgg
    23521 accgagcttg aggcgttcgc cccggaaggt cgtccacgcc ccaggcgccg gggtgcacga
    23581 gcggatcagt cgatcaacgg ccagcgcggg gagaccccaa tcgacccggg catcctcgac
    23641 ctccaccttg ggcgcatgcg agatcccttc gcccggttgg ggtttggcca ccaaatggcc
    23701 ggcctcgatg ccgtcgagag tagccacgag cagcccggct ccagcgagtg ccagccgccc
    23761 gagcaggtcg ccgctggtgt cggtcggtcg gatcgtctcg gtcatcacgc catagatcgg
    23821 gcccgtgtcc aacccctgct cgaggaggaa ggtcgtagcg ccggtgacct cgtcgcccga
    23881 gcggatcgcg tgctggaccg gtgcggcgcc ccgccaggcc ggcagcagcg agaaatgcag
    23941 gttgacccac ccgcgccgcg gaatgtccag ggcagcctgc gggaccagcg cgccgtaggc
    24001 gaccaccggg cagcagtccg gtgcgagcgc cgccaactcc tgctggaaat ccgggtcgcg
    24061 gggtgtgcgc ggagtgagca ccggtatgcc gtgctcctgc gcccgcacgc gcacgggcga
    24121 gggcagcagg ctgcggccgc gaccggccgg ggcatcgggg cgggtgacga caccgacgac
    24181 ctcgtgagat gacccgatga gagcctccag gctcggcagc gccacctcgg gcgtcccggc
    24241 aaagaccagg cgcatcgcgc tcacaccccc agaccgaagg tgttgtgtgg gctgatccgc
    24301 acgatcggtg ggccgccgac ccagtcggac tcgcggatgg ccttcatggc gagcttgcgt
    24361 tgggccgggt ccatccggtc gatgaagagg atgccgtcga ggtggtcggt ctcgtgctgc
    24421 acggcccggg cgagcagttc actgccttcc aggagcaccg gctcaccgtg ctcgtcctga
    24481 ccggtcgcca cgacgcgcaa cgaccggggg gtcgggtatt tcattccggg gaaggagagg
    24541 cagccctcct cgccgtcctg catctccacc gacaactcca gcgacgggtt gaccaggtgc
    24601 ccgacgacgc catcgacgaa ataggtgaag acccgcagcc cgacaccgat ctgcggcgcc
    24661 gccaatccag caccgggcgc gtccatcatc gtgtcagtga ggtccttcac cagttggctc
    24721 agctcccggt cgaagtcgac gaccgccgcg gcaggggtcc gcaggatcgg gtcgccgaaa
    24781 aggcggatct ccttgaccga caacgctgtt ccttcgagag ccaggaggcc gggcctggca
    24841 ccccgatcag ggctcgacac tcggcatcac ggaggtgcca gtctagtcgg ccgtttccgg
    24901 cccggcgtcg gcgccaacct ggcgcagccc ccgcaccgac ggtgcggcca gcgtggccag
    24961 gctgagcacg gcatacccga tggcgctgac gagcaggatc gagcggtggt cgtagacggt
    25021 ggcgagccac ccgaaggcca tcgccccgat cggcatcgcc acgaaggacc cgagcgcgtc
    25081 ataactggac acccgggaca acaccgcctc ggggatgtgt tggtgcagcg aggtgttcca
    25141 cccgacgccg aacacctcga ccccggcacc ggccacgaag aacgccgcaa tgagcaggtc
    25201 ggtgcgaggg gacagcccga gcacgagcat cgggagcgcg atgagcgaga ccccgagcat
    25261 ccccgcgacg agcgggcgcc gaaggcgagt ccgcatgagg gcgagggtca tgacgacggt
    25321 cccggcggcc tctgccgata acgcgaggcc ccagccctgc tcgcccaccg gcgaggcttt
    25381 cgcgatgacc gggccgagca cggcgatcgc ccccatgtgc acagcgttga gcactccgaa
    25441 ggcgacgacg atgagccata cccaggtgcg ggaggtgaac tccccccatc cctcgcgcaa
    25501 ctcggcgatg accgagggag acgccgtgac gttgcggtcg cggccgggca gggccacctg
    25561 ccgcagcatg gggatggcga cgaggaacaa cgccgagtcg accagcagcg cccaaccggg
    25621 cccggcgccg accacgaggg ctccggcgag cgccggaccg acgatggtga tggaacttcg
    25681 actgaaggac agcagggcgt tggcctgctg gatgcgatcg ggtcctacga cgagcgggac
    25741 gacccccatc atcgctggca tcccgaacgc ggacacggcg ccgttgaccg cctcaagcgc
    25801 gaccaggtgc cagatctgcg ccgtgccggt gaggacgagg tatgccgcca tcccctgagt
    25861 gaggaaggtc aaggcatgcg aggtctgcat gatgacggtc cgcgagtagc gatcggcgac
    25921 gaccccgccg agcaacagga agatgaccat cgccgccatg tgggcggcca ggacctgggc
    25981 gagggctccc gggtcatcgg agatgtgcag gacggcgaag gccagcgcga cgctggtcat
    26041 cgccgacccg atgctggtcg tcgtcctggc ggcgaagtac cacgcgaacc gccgctcacg
    26101 cagggcgcca acggcctcgg tcagcttcat cgaaacccct cccccgtcca gcccatccgg
    26161 acgttcagcg gctcggggtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26281 gaccggcaac gggccgaagg tggtgacgtc gaggtcggcg ccggacacga tgtcggacac
    26341 cgctgccgca acggcgtggc gggtgcccac caactcagcc atgacgaccg ccgggggcag
    26401 cccgagctcg gcccgctcgg tcaactcacg ctcggcgagc cacaccggat cccacctgac
    26461 gagggcttcg acggcgggga acggtgggcc gtcggggacc cccgcgagaa caacgacccc
    26521 accgtccagc gccgaccgac agagggcggc ggccgccagc caccgacgca acgcctcgac
    26581 gggggcgtcc aactcgggcc gactcaaggt ggcccaagca tccagcagca ggcacgcggc
    26641 atacccctgg gtggctcgcg gctccgcccc cggcgtcgcg atgaccagcg ccggggtggc
    26701 cgccacctga cccgccacct gaccggatgc cgaggtgatg accgggacgc cagggaaggc
    26761 tcgtcccagc tcctccgccg tcctgcggct gccgaggatc gccgcgcgcc aggccgtgct
    26821 gtcgcactgc ggacagcgga acccggcgat gccgaacccc gtgccgcaca ccgtgcacgc
    26881 cggggggtgg cgtggatcgt cttgacccat cgggccgtga caggcggtgc agcgcaccgg
    26941 tgctcggcag acctggcagg acagcgaggg gcggtaaccg ccgcggggca cctggacgag
    27001 caccgggccg cgggtcaacc cctcgtggac gatccgccag gcactcggtg gcaactgggc
    27061 cctggcagca gcgctgtcgc gctcccgccc gacctcccct tcgccggcga cgacgacccg
    27121 cgcggctgcc gcacggaggg tcgccggggt gggacgcagg tccaccactc gcccggccgc
    27181 cacccagcgc tgcacctgaa ccgagcggcc gaacccgccg accagcagcg ctgcggccga
    27241 ctgctcggcc cgaatagcaa ggacggtccg cacctgcggg tagggcgctc ggggctcggc
    27301 gagcagatcg tcaccgtcgt cccaccacgc gaccaggccc agatcgcgca cgggcgcgaa
    27361 ggccgcagtc cgggtgccga cgacgacgcg cacgtgcccc cgcaggagct tgagccaggc
    27421 cgtgtaacgc gcctgcggcc cttggtcggc ggtgaggcga acgtgccgac cagccccgag
    27481 gaccgcagtg agcgcctcgt cgacccggac gacatcgcgg tggtcgggga cgacgatccc
    27541 cgccccacgg ccggccgaca gcgcggccgc cgccgcctcg gcaaacgctc gcggccagtc
    27601 ctgctcgggg gcgcgaccag gcatggcaag ccaggcagcc cccggagcac tgcccgccgc
    27661 gacccgacgc aggaacgcct cacccgccgg gtatgccgcc cacggcccgg cctcggcgcg
    27721 ggtcggcttc tcggttctcg aaggctcggt cggcgatggc acgggtgggt cgaggggcag
    27781 cgcgcgttca gcggtggcat gacgcttggg gatcgcgagg cgcagcacgt cgccgaggac
    27841 cccgccgtag cggtcggcga ccgccctggt gagggcgtgg atctgcgggg tgagaacggg
    27901 ttcggggctc acgacccgac ggatcggcgt gagccggccg gggtgctcgg ccttcgctcg
    27961 gcgcgcgacg acgaagccgt ccagatcccg cccggcgaag cggaccctga cccgcacccc
    28021 cggttgggcg gcggcgtcca actcgggcgg gacggcatac tcgaacggac ggtccaggtg
    28081 ggcgagccca ctgtcgacga caacggctgc caccgggagg gggctggggg cgtcgtcgat
    28141 aggcacgctg gcaccctaac ccgcagggca gacaccgatt cgcgcggtca ctccggccgc
    28201 acggctcccg cgaacgagac catgccgggc agcaacggga cgagggtgca ctggtaggcg
    28261 tggtacaggt ccggcttacc ccaccagatc gcgtcggagg gttggttggt cggcgagagt
    28321 tcgtgtcgcc agcctccgcg gaccccgtcg aggaggtagc gatcggcata cgcccacaac
    28381 cggtcgtacc acgccagagc gtccggatcc ccggtgacct cgtgcagcgc ccacgcggcc
    28441 gcgatgccct cgcagacgac ccagtgcagc cgctcccgca cgatcgggac gtcctggaag
    28501 tcggtcgtgt agacgagccc ctcggctcca tcgacgtccc agccccgagc gaccgcggcc
    28561 tggaacatcg cagcggcgtc ctcccgcagg aacgcccact gcggcgcgtc ggcggcttcc
    28621 ccgagggtat gccgcgcgtt gagggccagc cgcgaccact ccaggacgtg gccgatcgtc
    28681 acgccgaagg ggcggaactg atcggcggga cggtcgcggt tgtagtcggg cagcacctgc
    28741 cattgggcgt cgaagtgctc aggcaggcgc cagtccgcgt cgcgggcgaa ctcgtggacg
    28801 acccgacgca ggatccgcag cgcgcggtcg atgtagcgct gatcgccgag cacctccccc
    28861 gcggcgagca tcgcctcgac ggtgtgcatg ttggcgttga cgccgcggta gtcctcacaa
    28921 cgggtccagg cgcggtcgta ctgctcgcgg gacatgcccg cgtcctcgtc ccagaagtaa
    28981 gtgtcgaaca cgtcgagggc ctcggtcagc agggccggcc ccccggggtg accggcggcc
    29041 gtcaggctcg ctgcggccag gatgacgaag gcgtgggcat aggccacctt gtctgtgggc
    29101 tgcggtgccg tgccgcctac tgcgggaagc caaccccgtg actcatggtc gcgaaggcgg
    29161 ccggtcagcg cgtcgtgtcc gacgtcgagc aaggggcgca ggtgcgccgc gccatcgcca
    29221 cggcgcatca cctcaagggc cgcgatgtgg gtcatccggc aggtgatcca ggtctcgacc
    29281 gggcgatcga ggacgacctc gttgtccggc cccagccagc cgaagccgcc atcgggatgg
    29341 acggcgcgag ccacgaggtc ggccagactg ctcgcgtggc ggtccaggcg acaggcctcg
    29401 gctgcggtcg gttcggcgct catgctggct ctccttgtcg gtggaacgtt cgagaaacga
    29461 cgcggtgggt ccagcgctcc ccgcggagca ctggacccac cgttcggtta cccgactgtt
    29521 aggtcagacg taggtcactt gaccaactgc gcgagggtcg ggtcgttggc gtaagcctcc
    29581 ttggcgttgg ccttggtcac gatgaccgga gccagcaggt tggccggaac gaccttgacg
    29641 ccgttgttgt aggacttctc gtcgttgacc gtgggcttgc cacccttgac gatcgcggtg
    29701 accatctcga tggtcgcctt gacgaggttg cgggtgtcct tgttaatcgt ggagtactgc
    29761 tcgccggcca tgatcgactt gaccgactcg acctcggagt cctgaccggt gacgaccggg
    29821 gacgccttgc cggaggcctt gaccgacgtg aggatcgcac gggccagggt gtcgttcggg
    29881 gacaggacgc cgtcgaggtc ggtcgccgag tagttggccg acagcagggt gtccatgcgc
    29941 ttctgggcgt tctcggcctt ccaacccggg gtgacggcct gggtaaagtc cttctgaccc
    30001 gaggcgacct tgagggtgcc gtcggcgatc ttcggggtga ggacgctcat ggcgccatcg
    30061 aagaagacct gggcgttggc gtcgtcaggc gaacccgcga acagctcgat gttgtagggg
    30121 ccattgggct tcttggcctt gagcccctcg aggagcgcgg tgccctggag cacaccgacc
    30181 ttgaagttgt cgtaggcgac gtagtagtcg accgccgcgg tgttcttgat gaggcggtca
    30241 tacgcgatga ccgtcgcgcc gccgtccttg gcagccttga gctgggtgcc cagctgcgag
    30301 ccgtcgatcg cgccgacgac aatgaccttg gcgcccttgg tgaccatcga ctggatctgg
    30361 ttctgctgct ccgagacgcc gccgttggcg aactggacgt cacccttgta gccggcagcc
    30421 gtcagcccgt ccttgaacag ctgctcggcg agcacccagt tctccgacgt cttctgcggg
    30481 agggcaacac cgatgagggt gccggcgccc gcggccgcac cggcggcacc cgaggtggcc
    30541 gtggtgctgc tgtcggaccg gccgcagccg gtgagggcca gggcggcaac agcgctgacc
    30601 gccagaaggg acttgatggg tgtgcgcatt ctgtttctct ctctggttgg tgagactaat
    30661 tcgaggtgag acgtacgcga ggaccgggac ggtcgcgcgt ggatgaaggt caggccgcct
    30721 tgtcggatac cggcgccgcg gccttcttgg agttgcgcat cagcagcccg atgatcgacg
    30781 ggcgcccctg catcttgttg tagacgtcga aggcaacggc gatgaggagc accaggccct
    30841 tgatgatctg ggtgaggtcg gccccgacac cgagcaactg catgccgttg ttgaggacgg
    30901 ccatgaccag accaccgacg atcgacccga tgaccgtgcc cacaccaccg gtgacggcgg
    30961 cgccaccgat gaagacggcg gcgatcgcgt caagctccca gccgaccccg tcgaacggac
    31021 cggaggcgtt ggagcgagcg acgaacatca tcccggccag ggcggcgagg atggacatgt
    31081 tcatcatgac gaggaagttg acgcgcttgc tgcgaacgcc ggagagctca gccgcgtggc
    31141 ggttgccgcc gactgcgtag acgtgccgac cgatgatcgt gcgggtcgag atgaagccgt
    31201 agatgagcac caacgcaccg aggatgaggc ccgacacggg gaacgacgtg cccttacggc
    31261 ctgaggccat gaggagggtg accgccagga tggccgcgct gatcaagccc gtccggatcc
    31321 acagcgccgt ggccgagtcg gtggccgcgc cgatcttgac ctggttgcgg cgggtgcgga
    31381 cggcaccgaa gacgaccgac gcgacgagga tcaggcccaa caggacggtg aggttgttgt
    31441 agcccgtgtt cggcccgatc tcgggcaggt agccggcacc gatgacctgg aagtcctcac
    31501 cgaccgggat ggtgttcgac ttaccgatga gctggttgag cccgcggaag accatcattc
    31561 ccgacagggt gacgatgaag gccgggatgc ccacgtaggc gacccagaag ccctgccacg
    31621 ccccgatgat cgcccccacg accagcccga cgagcacggc gacaacccag tgcatcccgg
    31681 tgtcacgcat caacagtgcc acgacgatgc cgaccgcggc cgccaccgag ccgaccgaca
    31741 ggtcgatgtg gccggcgatg atcaccagga ccatcccgat cgccatgacg aggatgtagg
    31801 agttgccgtt gaagatgttg atgacgttct cgggcttgag gacgagcccg ttggtcatga
    31861 tctggaagaa cacgatgagc gcgacgagcg cgaagatcat gccgaactgg cgggtgtctc
    31921 cgccgaagat gtccttgagt ttcttcatca agagtccttg ggtgtcaggc tgacgcggcg
    31981 gcggagcggt cggaaagggt ggtcatcaac ttcatcaggt tctcttgggt ggcctcgccc
    32041 ttggccagac accccgtgat ggcgccctcg cagatcgtgt agatccggtc ggagaggccc
    32101 aggagctcag gaagttccga ggagacgacg atgaccgcct tcccgctctt ggcgagttcc
    32161 tggatgatcc cgtagatctc gtacttggcg cccacgtcga tgccacgcgt cggctcgtcg
    32221 aggatgagca gctcagggtc ggtgaacagc cacttggcca ggacgacctt ctgctggttg
    32281 ccgccggaga gcttgccgac cccttcgttg accgtggggg tcttggtgcg aagcgacttg
    32341 cggtagccct cggccgcttc gaactcggcc cgacggtcga ccaccgagtg cttggagatc
    32401 ttcttcagct tcgccgacac cgtagtcgtc ttgatgtcgt ccagcaggtt caggcccagc
    32461 accttgcggt cctcggtgac gtagccgata ccggcgtcga tcgctgccgg gacgctcctc
    32521 aggctgaccg gcttgccgtc gaggaacagc tcgccggagc ggaagatgcc ataggactga
    32581 ccaaagatcg accgcatgag ctcggtacga ccggcaccca tgagcccggc gaagccgacg
    32641 atctcaccgc gacggacgta gaagttggag cccttgcaca ccatgcgccc cgggatgacc
    32701 gggctctcca ccgtccagtc gcggacctcc acgaacttgt caccgatggc gggggtgtag
    32761 tccgggaacc gcgagctcat ctcgcgcccg accatcccgc ggatgatgcg gtcctcgtcg
    32821 accttgtcgc ccttgacgtc gagcgtctcg accgactggc cgtcgcggat gatggtgatc
    32881 gagtcggcga tctgctcgat ctcgttcagc ttgtggctga tgatgatgca ggtgatgccc
    32941 ttgcggcgca gctcgtcgat gagaccgagc aggtgctgcg agtcgtcctc gttgagcgcg
    33001 gcggtgggct catcgaggat gagcagccgg acgtccttgt tgagtgcctt cgcgatctcg
    33061 acgagttgct gcttgccgac gccgatgttc ttgatcgcgg tctggggctc gtcgttgagg
    33121 ccgacgtgcg cgaggagctc gcgtgccttg cggttggtct cgacccagtc gatgacaccg
    33181 ctcttgacgt actcgttgcc caggaagatg ttctccgcga tgctcaactc agggatgagg
    33241 gcgagctcct ggtggatgat gacgatcccg gcatgttcgg actgccggat gttcttgaac
    33301 tcgaccggct tgccgtcgta gatgatctcg ccggtatagc tcccgtgcgg ccacaccccc
    33361 gacagtactt tcatgagggt cgatttgcct gcaccgttct ctccgcagat ggcatgcacc
    33421 tcgccccggc ggaccaccat ggatacgttc tcaagggcct tgaccccggg gaactccttg
    33481 gtgatgttgc gcatctcgag gatgacgtct gacaccagtt gccaccttcc cacgtcgctc
    33541 agcaccgagc gcctgccgtg acggcactgg cgcggcccct aacctactcg gccacgccca
    33601 agccacctag agctatttgt tatcccgatc aacttattgc tgggacaatg gctcgtcaag
    33661 gccgccccgc gccgcggtgg gtaacgattc gatcaccaaa cgacgggccg gcaggggctc
    33721 acctcggggc cagccagggc gccgggtggg caacgacgcg gtcgacgaca cgcagcgccg
    33781 cgccactcaa ggaggcgtag tcgccgctga cggccgactc cacccgcacc ggcacccacc
    33841 gtgcggccag cactcgctgg ttgatgatgt cggcaaccaa cggaccgagg acgtcgaaca
    33901 gttgggcata gaagccgccg agcacgactg cgtccaggtc cagcaggttg agcgaggagg
    33961 aggacgcgag tcccagcgcc tcggcggcct cggtgagcgc agcggtcgcc tggctgttcc
    34021 cggcccgcgc ccgagccagc aggcgcgaca ccgacgtgtt ggccgggagg tgcgctcggc
    34081 gcagaatggc gtccttgccg gcatactgct ccaggcaacc tgttgcccca caacgacatt
    34141 gcggacccgc agggtcgagg acgctgtgcc cgatctcgcc gctccacccg tgcaggccag
    34201 tggcaagatc gccgcccaga acgatcgcgc cgccgatgcc gacctcacca ctgacgaaga
    34261 ggaagttctg gtccagccgc gaccctcccg tcgcccggga ccggtgccgc gcctcggcgc
    34321 gcgcggccag gttggcgtca ttacccacct cgagcgtgag atcacgcaac cgggcgctgc
    34381 gtcggagcac cgcgagcgca tcgaactctc gccagcccag gttgggggca tagcggacga
    34441 tcccgccctg cggatcggtc agaccgggga ccgacaaaca caccccggca actgtcgcgt
    34501 tgagtgaatc cagttgggcg acaacaagtt cggtaagcga tgtcactcgc tcgagcatgg
    34561 cgagcggttc actcgaccgg aagtcgcctt cgacgatccg ctcggtgact agggtcccgg
    34621 ccaggtcgag tgcacggaca ccgatgtgat cgacggcgac gtcgacaccg accccgatga
    34681 acgatccggg cgcgggcagc aaagggacac ccgggcgccc ggcccggtcg acgctctcgg
    34741 gtgcggcttc aaccaccagt cgcgcagcga tcaatcggtc gaccagatcg gacaccgccg
    34801 gacgggtgag gtcgaccgcc ttcgccaacc gggcgcgtgt catcggcgtc cccgcgtcga
    34861 cgatgtggcg caggacgagc gaaaggttgt gttcacgaag tgaggactgc cgcgcagggc
    34921 ggacgttacc tcggctgcgc agcagagccg gctgacccgg ctcatcgcgt cgaagagggc
    34981 tcaccatggg gccgaccata ccgcgcgata ccccgatttt gttcgtcccc ggaacaaaca
    35041 aacgatggac ggctcgatca gcggctgacg gccgcgcgca aggcgtcgac ccgatcggtg
    35101 cgctcccagg tgaagccccc gtccgcgtcg gttcgcccga agtgaccgta ggccgcggtc
    35161 cgctgataga tcgggcgcag cagatccaac tggtccacga tcgcggccgg acgcaggtcg
    35221 aagaccgacg cgatggcccg ctggatcgag tcgaccggga ccgtctcggt gccgaacgtc
    35281 tcgacataca gacccaccgg gtgagcggct ccgatggcgt aggccacctg cacctcgcag
    35341 cgccgggcca gcccggcagc cacgacgttc ttggctaccc accgcgtggc gtatgccgcc
    35401 gagcggtcga ccttcgaggg gtccttgccg gagaacgcgc cgccaccgtg ccgcgccatc
    35461 ccaccgtagg tgtcgacgat gatcttgcga ccggtgaggc ctgcatcgcc catcgggccg
    35521 ccgacctcaa accgaccggt ggggttgatg agggcgcggt aggacgacac gtccaggtcg
    35581 ctgccggcgt ccttgagctc ggcgagcact gggtcgatca cgtgcgccac gatgtcggga
    35641 gccaacagcc cttcgatcga cacgtcggcc gcgtgctggc tggacaacac cacggtgtcc
    35701 agccgaaccg cccggtcgcc gtcgtaggcg atagtcacct gggtcttgcc gtcgggacgc
    35761 aggtagggca actcctgcgt cttgcgcacg gccgtcagcc gctcggccag ccggtgggcc
    35821 aggtggatcg gcaggggcat gaactccgcg gtgtcgtcgc acgcgaaccc gaacatcagg
    35881 ccctggtcgc cggcaccctg cttgtcgtag acatccagcc cgccgtcacc ggtgcggttc
    35941 tcataggcgg tgtcgacgcc ttgggcgatg tcggggctct gctggccgat cgacacctcg
    36001 acgccgcacg acgctccgtc gaagcccttg gtcgatgagt cgtaaccgat cgccagcacc
    36061 gtctcacgga cgatggacgg aatctcgacg taagcctcgg tcgtcacctc tccggcgacg
    36121 tgcacgaggc cggtcgtcac catggtttcg acggccacgc gcgaacgggg gtgctgggcc
    36181 agcagggcat caaggatggc gtcactgatc tggtcgcaga tcttgtcggg gtgaccctcg
    36241 gtgaccgact cggacgtgaa caggcgggcg gacactctcg actccagctg gtggggatct
    36301 gaggaaccac cgcgcaggcg atggcgtggc ttttcagtct agtgcgcggc gggaagcaga
    36361 acctgtatgc cgtcccacac cgccgcggcg acatcggact tggtggccgg tcccaccgtg
    36421 acgacgggac gaccatcggc gaaaagcagg tggaccgtgt tggtgtcttc gccgaaggtc
    36481 ttggtggcgc cgacctcgtt ggccacgagc aggtcacacc ccttgcgagc gagcttgacc
    36541 cgggccaact ccacgaccga tccggtttcg tcgccggtct cggctgcgaa gccgacgatg
    36601 atcggccggg cggcggcgcc ccgcccgacc acgaggccgg cgaggatgtc gggattgcgg
    36661 accaactcga tcaccggggc cgagtcgtcc gatccgtcgg ccgcatgggt cttcttgatc
    36721 ttggtctcga catagtgctt gggccggaag tccgccacag ccgcacacat gacgacgacg
    36781 tcgacatccg cgcaggcagt ggtcacggcc gcttgcaggt cgagggcaga ctcgaccggc
    36841 acgacccgga tcgcaccgcc ggagcccgaa ccgccgaccg ccgcgctgag gttgcacgcc
    36901 acgagcgtca cctcggcgcc acgcgccgcg gccacctggg ccaaggccac cccttgtttg
    36961 ccggaggacc gattgcccag gtagcgcacg ggatccaggg cctcacgggt gccccccgcg
    37021 gtgacgagga cgcggacgcc gcgcaggtcg ccgcgcacgt cgccgtgcgg gtcgtcgccc
    37081 gctgcctcgg cacgcactgc cgggtcggcg gctgcgacga cggccaggca cgcggcatac
    37141 aggtcctcgg gctccggaag cctcccagga ccggtgtcgg cccccgtcag ccggcccgac
    37201 gaaggctcga tgacgtgcgc gccgcgcgag cgcagggtgg cgacgttggc ctgggtcgcc
    37261 gggtgaagcc acatctcggt atgcatcgcc ggcgcgaaga cgaccgggca gcgtgcggtg
    37321 agcagggtgg cggtgaggag gtcaccggcc tgaccggtcg ccgccttggc caacaggtcg
    37381 gcggtcgccg gggcgacgac gacaaggtcg gcatcctggc cgatgcgcac atgcgggacc
    37441 cggtcgacgg ccgtccacac gtcggtggcg accggatgtc ccgagagcgc cgcccacgtg
    37501 ggcgcaccga cgaactgcaa cgcagcctcg gtggggacga cggtgacgtc atgaccgctc
    37561 tcggtgaacc gacgcaacag ggaagcggcc ttgtaggcgg cgatgccgcc gctcacgccc
    37621 aggacgacgc gcatgatgct ctggctccga tcggtcgacg tcgcccgacg gggtctgctg
    37681 gctcagccct cggtggcctg cgcggtgagc aggccttcgt tgatctcacg cagcgcgatc
    37741 gacagcggct tctcgtggac ctcggtctca acgagggggc cgacgtagtc gagcagaccc
    37801 tcctgcaact gcgagtagta ggcgttgatc tgccgggccc gcttggcgga gtagatgacc
    37861 agcgcgtact tgctgtcggc gatggcgagc aggtcgtcga tgggcgggtt ggtgatgccg
    37921 atggggttgg ccacggttcc ggacacggcg aggatctcgt ttcgatcggg ggcttgcggt
    37981 cgctaggggc gccttcacgc agaaggacgc gatcccatca atgatacgag ttgttcactg
    38041 gcccggcgga catcgtcgtt gacgatgacg acatcgaact cttcggccgc cgccaattcg
    38101 cgggtggcgg tcgacaaccg gacagtccgc tcctcctcgg tctcggtccc ccggccgacc
    38161 aaccggttga ccaactcgtc ccaactcgga ggggcgagga agaccagcag cgcgtcagga
    38221 aggttgtcgc ggacctgccg ggcaccctgc aggtcgatct ccaacagggc cggacgcccg
    38281 gcggccaggg cgcgctcgac gggaccacgg ggggtgccgt acttcgcgcg accgtggacg
    38341 acggcatact cgaggaactc ccctgccgcg accagccggt cgaactcctc gtcggcgacg
    38401 aagatgtagt ggacgccgtc aacctcaccg ggacgcggcg cgcgcgtcgt catcgagacc
    38461 gacagccaca cctggggata gtgggtgcgg atgtatgccg cgatcgtccc cttcccgact
    38521 gcggtcgggc cggcgagaac gaccaaccgt ccggcacctc cttcggggag gatgaacggt
    38581 tcggcgaggg tgtcctgccc gctcacgact gatcgaaccg ggcgatgagg gccgccgact
    38641 ggttgacccc cagcccgcgg acccggcggg actcggagat cccgacctcg gccatgatct
    38701 ggcgggcccg gaccgggccg acccccggca atgactcgag cagcgccgac accttgagtt
    38761 tgcccagcac cgggtcgtcc ttgccttcgg cgatcacctc ggcgagggag ccctgggagt
    38821 acttcaggcg gttcttgacg gcggcccggg cccggcgggc gagggcagcc ttctcgagag
    38881 cagccgctcg ttcttgcggg gtcagctcag gcagggccac cggtcactcc tcgatcgtgc
    38941 tctgggacga catcaaacac cgaacctacc gacgcgtccg gcccgtcgca acgacaggcg
    39001 aaagttcatg cctccctgag cctgcgcagc cccctgcggg atcacagagc ggtcctccgg
    39061 cgggcaacac cgcaggtcac gacgccagag cctcggccag ggccgcagcg gtgtcctgtg
    39121 cccgggtggc cagcgcggcg acgctggggc cggcgctgag gacctcccgg ctggacgcgg
    39181 ccaggacatg cgggagcgcc gtgccgaaga cccggcgcag gctctcgacg gtgcctcctt
    39241 gcgccccgac cccgggagct agaaagggac cgcccatccc ggccaggtcc agacccaacc
    39301 ggtcgacggc gtccccgacg gtcgccccga ccacgagccc gaccgagccg agctcccccc
    39361 gggccaccgc gtccgcgttg tccgcggtgg ctcccgccac gacatgcccc gcgacgctct
    39421 gcccatgcag gacggcgtgc tggacgtctg cgccctccgg gtttgacgtg agcgcgagca
    39481 caaagacacc gcgcccggtc gcgtgggcga ggtcgatcgc cggccgcagg gagccatatc
    39541 cgaggtaggg gctcagggtg atggcgtcag gaggctcggg cgcgtccttg ccgaggaacg
    39601 cctcggcata ggcgtccatg gtcgtcccga tgtccccccg cttgacgtcg aggatcacca
    39661 acgtgccggc agcccgcagg tcggcgacga gtcgttcgag cacggcgacc ccggccgagc
    39721 cgaagacctc gaagaacgcc gattgcggct tgaccgcggc cacccgcccg gcgaaggctt
    39781 cggcgcaggt catcgcgaac cgctccaacc cggccaccgt gtagtccaat ccccaggcct
    39841 gcatcagccc gcggtgcgga tcgacgccgg cgcacagcgg cccgtaggcc cgcatcgcct
    39901 ggtgcaagag cacgccgaac gacggcggat gcagcagcga cggggcctgc gcagaagtgg
    39961 tcattgacgt ctccgttcgg ggtgggctgc cgcgacgaca tcggcgaacg cggcataccc
    40021 acgctcgtcg agcagtcctg cgagctcatc gaggacgcgg accggggcct cggggtcgtt
    40081 gaaggtggcc gtgcccacct ggatcgcact ggcacccgcg gcggccaact cgagcgcgtc
    40141 gcgcccggac gccactcccc cgacgccgat gatcggtgcg gtagggatcc gctcggcgaa
    40201 catcgcggcg cggacctgcc agatggcccg aaccgcgatg ggccggatcg ccgggccgct
    40261 gaggccgccg gtgatccccc cgaggtgcgg gcgcatccga tcgagatcga tggccatccc
    40321 cagcgtcgtg ttgatgagcg tgaggccgtc ggcacccgcg cccaggcacg ccgaggcgat
    40381 ggtcacgagg tcggtgacgt cggggctgag cttcgcgaac accggcaggt gcgcagggac
    40441 ggcggcacgc actgcggcga tgaccgcagc ggacgcgagc gggtcgatgg cgaagaccaa
    40501 cccgcggctg gcgacgttgg gacacgagat gttgacctcg atgcccgaca ccgacgcggc
    40561 ggccgggctg gagagcagtc tcgttgccac ctgggcgaat tcgtcaggcg tgccacccgc
    40621 gatcgaggcc aacacgcggg cgcccaccga ggccagccac gggaggtcct cgtcggcgaa
    40681 ggcatcgatg ccggggcctt gcagaccgat cgagttgagc atgcccgacg gtgtctcggc
    40741 catccggggc gttccacggc cgctgcgggg agcgggcatg acggtcttcg tgacgaacgc
    40801 tcccaaccgc gagatgtcga agaaccggtg caactccttg ccgttggccg cgcaaccgga
    40861 cgccgtcatc agcgggttgg gcagcgtggc tccggccagc gtcgtgctga gatcgaccat
    40921 cagtgccctc ctgcctgcgg cgctccgacg gcgtcgtccg gcacccggca caacccgtcg
    40981 ccgaaggcgt cccaccggac ccgatcgcca tcgaagaccg gaccgtccag gcaggcacgc
    41041 accatgcggg tgcggccgtc ggccccgatc accggcatga cgcaggtcat gcacacgccg
    41101 acgccacagg ccatcgcctc ttcgacggcg acgtgggcgg ccgcgcctgc cgcgctcgca
    41161 gcctcagtca cagcacgcaa cattgccatc ggaccgcagg catagacgtc tgtggcacca
    41221 gatcgggcca atacctgggg aagcacatcg ctcacccagc cgcgcgttcc ggcggacccg
    41281 tcgtcggtcg tcacctcgac ggcatcggcc acctcggcgg ccagggcgac gccgaacagc
    41341 cggtcggcgc tggctgctcc gaggacgatc tcgacgtgga cgccgcgctg gcgcaggacc
    41401 tgggccagcc agaagagcgg cgcgcttccg taaccaccgc cgacgagcac ggcagcaccc
    41461 ccgccgtcag gcagggggaa cgcttgcccg agcggtccga cgagatcgag gacatcgtcg
    41521 atccggcgct cggtgatcca cgtcgtcccg gcggcgtggg ccgcgacgac gacctcgacg
    41581 gtccccgcgt cggggtcgac ggcgtggatc gagaaggagc gacgcagcag catccccgat
    41641 gtcgccccgc cgacggtggc tgccacgaac tgtcccgggc gggccagcgc ggcgatggcc
    41701 ggggccgcga cgacgaggtg ctggtaggca ccggcgggct cactggagcg caccggggcg
    41761 cgctcgtgcg cgacgtcgcg ggctgagacg ccgacggggt atgccgcgcc gctcaccgcg
    41821 ccacgccatc ggggttcgtg atgccgtaga ggttcagggc ccgggcgtgc tcctgcaagg
    41881 acgtgacccg catgggtgcc gagcgcatcg actcgatgcc ctgaaccgca gcgccaagct
    41941 cttggaccgt cgtgatgagc gtgacgtcgg ccgcgatggc ggccgcccgg atggcgtagc
    42001 cgtccgcgcg ggccgtctgc ccggtggggg tgttgacgac catcgcaaca tctccggcga
    42061 tgatccggtc ggcgatggtc gcctcgatca cgtccccctc gccgactggg ccatcacccg
    42121 tccgctcggt gatcttgcgg acgacctcag ccttgattcc gttgcggcgc aacacatctg
    42181 cggtccccga ggtggcgagc agggtgaagc ccagctcggc caacgcccgg accgggaaga
    42241 tcatcgcccg cttgtcacgg ttggcgaccg acacgaagac ggtcccactc gtgggcagcc
    42301 cgccgtaggc ggcgagttgg ctcttggcga aggccgtccc gaagtcggtg tcgatgccca
    42361 tgacctcgcc ggtcgacttc atctccgggc cgaggagggc atcgaccatc tgcccttcct
    42421 tggtccggaa ccggttgaac ggcagaacgg cttccttgac cgacatcggc gcgtgggccg
    42481 gcaaggtccc gccgtcgccc gtgggcggca gcaagccttc ctcgcgcagc gcagcgatgc
    42541 tcgagccgag catgatgcgc gccgccgcct tggccagcgg caccccggtc gccttggcga
    42601 cgaacggcac ggtccgagaa gctcgtgggt tggcctcgag gacgtagagg acgtcctgag
    42661 cgagcgcgaa ctggacgttc atcaaccccc gcacgccgat gccgtgggcc aaggccagcg
    42721 acgaggcgcg cacccgctcg atctcggccg ccccgagggt ggccggcggg agggtgcagg
    42781 cactgtcacc ggagtggatg cccgcctcct cgatgtgctc catgaccccg ccgagataca
    42841 tgtcgacgcc gtcgtagagg acatcgacgt cgatctcgat ggcatcatcg agaaaccggt
    42901 cgacgaggat cgggtgctcg ggcgaggcga tggtcgccgc gtcgacatag gtcgtgaggg
    42961 tcgcgtcgtc gtagacgatc tgcatcccgc gcccacccag gacgtaggac gggcggacga
    43021 gcaccggata accgatctcg tgcgcgaccg cgaccgcctc ctgcgcgctg tatgccgtcc
    43081 cgtgccgggg cgcgggcagg ttcgcctccg cgaggacccg gccgaaggca cctcggtcct
    43141 cggcgaggtt gatcgcctcg ggcgaggtac cgacgacgag gactccctcg tccttgagag
    43201 cctgagccag accgagcggg gtctgcccgc cgagttggac gacgaccccc gcgatcggac
    43261 ctgccgcagc ctctgcgtgg acgacctcga ggacgtcctc aagggtgagc ggttcgaagt
    43321 agagccggct cgaggtgtcg tagtcggtcg acacggtctc ggggttgcag ttgaccatca
    43381 ccgtgtcgta gcccgcgtcg cgcaacgcga acgacgcatg gacacaggag tagtcgaact
    43441 cgacgccttg accgatccgg ttggggccgg agccgaggat gatgacggcc ggccgctctc
    43501 gcggcagcac ctcggtctcg ctgtcgtagg acgagtagta atagggcgtc gcggccgcga
    43561 actcggcggc gcaggtgtcg acggtcttgt agaccggccg gatgccgagc gcatggcgca
    43621 ctcctcggac gacggcctcg ggcatggccc gcagcgaggc cagctgccgg tcggagaacc
    43681 cgtgtcgctt ggcgagcgcc agcaactcgg gcttgagccg ctcggcggtg cgcacggact
    43741 cggcgacctc gttgatgagg gcgatctggt cgaggaacca cgggtcgatg ccggtcgcgg
    43801 catacacctg ctccacggtg gccccgccac gcagagcctg ctggaccccg atgatccgcc
    43861 cgtcggtcgg gaccgcgatc tgggcgagca acgcctcgac cgccgccgcg tcgggaaccg
    43921 ggccgtccca gtggaagctg gcctccttgc gctccatcga gcgcagtgcc ttctgcagcg
    43981 cctcggtgaa gttgcggccg agcgacatcg cctcgccgac actcttcatc gtcgtcgtca
    44041 gggtcgggtc ggcggcgggg aacttctcga acgcgaaccg cggcaccttg acgacgacat
    44101 agtccagggt cggctcgaag gacgccggcg tctgcttggt gatgtcgttg gggacctcgt
    44161 c
//
LOCUS       DAORPW010000006        37837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_137_length_37837_cov_681.869726, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000006 DAORPW010000000
VERSION     DAORPW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 37837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37837
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137_length_37837_cov_681.869726"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1256
                     /gene="rocD"
                     /locus_tag="PLB86_01265"
     CDS             42..1256
                     /gene="rocD"
                     /locus_tag="PLB86_01265"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRC63795.1"
                     /translation="MTSAPHSSADFIELEHRFAAHNYSPLDVVIARGEGAWVEDVEGR
                     RYFDALAGYSALNFGHSNPRLIEVAERQLRTLTLTSRAFHSDQLGPFARDLCALTGKD
                     LILPMNSGAEAVETAIKVTRRWGKRTKGLPEDWGTIIVMGGNFHGRTTTIVSFSTDKV
                     AREDYGPYTPGFLVVPYGDIDALRAAMTDETIGVLLEPIQGEGGVVIPPEGFLREVRA
                     LCTERNVLMVADEIQSGLARTGRTFACDHEDVVPDIYILGKALGGGIVPLSAIAANTD
                     VLSVITPGSHGSTFGGNPFAAAIGREVIAMLQTGEFQERARLLGDLLAGELAALNHPG
                     IVEVRLRGLWAGIDVDPALMSGKELCHRMADRGVLAKDTHGSTIRLAPPLVATEADIR
                     MLVSVIADSLNA"
     gene            complement(1343..1543)
                     /locus_tag="PLB86_01270"
     CDS             complement(1343..1543)
                     /locus_tag="PLB86_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRC63796.1"
                     /translation="MTWTMRVDWPSCAARGLCAELLPERIHLDEWGFPVVEGTVTPDL
                     LGLAKAAATACPHRALRLVSAG"
     gene            complement(1540..2898)
                     /locus_tag="PLB86_01275"
     CDS             complement(1540..2898)
                     /locus_tag="PLB86_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRC63797.1"
                     /translation="MSGAVLASVTSFPGAGAGAPVAPSAPAATVRRSLSARSVPRTVR
                     TDPPAQVVATPGPVLLSGVLDGPSLEAHRDRFGQLPQLDLDTVLATVGAGAVVGRGGA
                     GFPFARKLAAAATGRRRAVVVINLAEGEPASAKDTALALVAPHQILDGAEVVARALRT
                     TEVHIVTPLERPAVGDAIAAALSERAAQPGRRDRSQPRWRMRAAEPGFVSGQARAVLE
                     LLAGRPGLPVTAWKPEAMSGLDGRPTLLSNAETWAQVAAVLRMGALGYAAHGTDTEPG
                     TTLLTLPHRRDADTAVVLEAAYGTPWVDVLGPDVEQPVLLGGFHGMWADAGVLAEATV
                     SREELRARGLGLGAGVVLPMRADQCPVAVTSEIVDYLAKASARRCGPCLNGLPALAEE
                     MRLVALGETSASWTRIGELVGLVDKRGACAHPDGTARLVRSLLTTFDDEVALHLRGGC
                     SR"
     gene            complement(2895..3461)
                     /locus_tag="PLB86_01280"
     CDS             complement(2895..3461)
                     /locus_tag="PLB86_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HRC63798.1"
                     /translation="MSLPSIDLSGPLLWFANRGTGIVLLVLYTAVVLLGILSATGRGP
                     GRFVPRFVTQGLHRNLALLATVLLAAHVTTAVVDTYVDIRWFDAFLPVGGLYRPLYLG
                     MGALSLDLLLAIGVSSLLRSRISERVWRRIHLLAYVSWGVAVIHTLGIGTDAGSPWGR
                     WTVLSCVAAVVVAVAGRAVARRVQVVRS"
     gene            complement(3458..4483)
                     /locus_tag="PLB86_01285"
     CDS             complement(3458..4483)
                     /locus_tag="PLB86_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRC63799.1"
                     /translation="MTLALRAATPQPPAESRVRRGAGGTAYDVPTDPGSRSWRALGTY
                     VHLGVADATVADEAAALAAAVLSEVDVACSRFRQDSDLVRANQAGGHWVSVSPILIGA
                     VRVALRAAHSTDGLVDPTLGELMVAAGYDRTFSQLPSTVEAHALPVPVAGAWSDVRID
                     GDRVRVPHGVALDLGATGKAYAADLVALTIVERLGVAVVVSVGGDVRAHAPAGAAVTW
                     PVDLAPDLRAWSDNDPEVCRVSLTAGGLATSSVTARRWVRAGSAWHHVLDPRTGRPAQ
                     TPWLSVTAYGDCAADANTATTAALVLGADAPEWLAQHGVAARLLAADGSVRRTPTWPT
                     DLEESRT"
     gene            complement(4480..4860)
                     /locus_tag="PLB86_01290"
     CDS             complement(4480..4860)
                     /locus_tag="PLB86_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63800.1"
                     /translation="MHALTGVVAAATVASTGAITGLAAADTAQQQALKAQAKAAAAQQ
                     TAAEIVDRTYRTVVSESVTTAAATGGSLSPSPKPKTSASRTTTVAKPVAAPKPVAAPK
                     PVVAPKPAAPKPAPKPAPVVSSGS"
     gene            5164..5586
                     /locus_tag="PLB86_01295"
     CDS             5164..5586
                     /locus_tag="PLB86_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63801.1"
                     /translation="MWIGWKAISLGVVSGGAIAVALYSSLAAGSAAPSASIPISQTVP
                     TAVYADCVEPAVLEGDECVTHVSVTRTVAAPAPAAVPVPAVAASSATTSATRYTPAPT
                     PEAAPAAAAVDDHDDDEHEDERDGDDEPDDDERDGDDD"
     gene            complement(5630..7048)
                     /locus_tag="PLB86_01300"
     CDS             complement(5630..7048)
                     /locus_tag="PLB86_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC63802.1"
                     /translation="MLRSRITWLAAALVAGVVISFVIPLCLLVRTMAEERAVSATNSD
                     AQSVAMIVAGVDDPASLPAVLAPTLGARTATTTVALPSGQLIGGPTTTGPVTTGQPSS
                     GPAQFPEDARLLAAVRADNRSMATTVDDRFHVLIPVGVADGVAIVHSVTDLGRVRRGV
                     YGSWAILVAVGLLLVVVSVLVARRQSARISTPVVAMAGVAHRLRSGDFEARAAATGPV
                     EVVELGHALNQLADRIEDLLRIEREGAADLSHRLRTPVTALRLDTDQVEDPTVRARLR
                     SHVEDLHRAIDSVVADARRPSRDAMGGWCDARVVLADRAHHWGPLAEDQGRMTTVIGG
                     SANGSASGAAGESVTGEHPGGEHAAYRPALVGLAARDLADLLDNLVDNAFAHTPEETP
                     IRLELHVVDGLVVLRVEDGGDGSPDTVAFARGESGAGSTGLGLDIVRRLAADAGGSVG
                     FERAGLGGLAVIVVLPLIERAD"
     gene            complement(7048..7752)
                     /locus_tag="PLB86_01305"
     CDS             complement(7048..7752)
                     /locus_tag="PLB86_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC63803.1"
                     /translation="MARVLVVEDDPAIRGALLRSLAGAGHAVTSQPTGMAGLTEAVDQ
                     PPDVVVLDLGLPDVDGLTVLSMLRAVSSVPVVVATARDDEKEIVRALDLGADDYLVKP
                     FGGDQLEARIRAVLRRAGHDAPPTPIVVGGLVITSASREVTVDGIPVELSRKEFDLLH
                     LLAQRVGEVVTKREILTEVWRQSYGGGDRTVDVHLSWLRRKLGETAADPVYLHSVRGV
                     GVRLVDPRGAHPGSAD"
     gene            7788..8315
                     /locus_tag="PLB86_01310"
     CDS             7788..8315
                     /locus_tag="PLB86_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63804.1"
                     /translation="MSGSRAQLLRYAATWVLVVSVTAGLSWGAISRAGEAATLLGAPV
                     PLGSAAAPTTAGLTPSATQSVPPPPPVTSAPTSSGQSSTSPSSPSTPKPEPTESPRPV
                     VRSVSTAAGQVWAYCVGDTAYRRSVVATDGWSFEFSREDNGRFEVKFQLAGAEEEEVE
                     VKVRCEAGLPVFTTD"
     gene            complement(8363..9244)
                     /locus_tag="PLB86_01315"
     CDS             complement(8363..9244)
                     /locus_tag="PLB86_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63805.1"
                     /translation="MIDTGSNRFSGELRVALHASGLTLDAVQRRLAERGFAIGRSTLS
                     YWQNGHRLPTQPTSLLAVTALEEILKVPIGSFTEALRHPNSTPVSPELDMAAAAARIG
                     VLMEGVGCPDGMATLEPLSAIQVAEFGPTGCLRSVRAVETYRALVDTDRFPILHGGEP
                     GGRPELIRHEAVSGGRIGRVRRDAEANVVVSELVFDRFVRRGEHHFLHYLTFDDNEQR
                     SLLLYRLVVTSRSMMVLDLGFHPDCLPVRLEEFERATDNGPDLFVRQRTLSPDRRVTL
                     VRERARRGIVGLRWEYA"
     gene            complement(9581..10447)
                     /locus_tag="PLB86_01320"
     CDS             complement(9581..10447)
                     /locus_tag="PLB86_01320"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRC63806.1"
                     /translation="MTTPTKAPSIVLSPRLGGGRTLESVARRCLEHTDANEPLLDAHG
                     DHEALHQFRVGYRRLRSLFSLAREVSRADPEARRLKAQLRELTVAFGPARDLDVFLEG
                     HPDLSPTNAARLEDARVTAYSEALEFLHSPEWAQTRVDLDEWLRNGSYRDLLGTGTWS
                     GRAWTAGALERRRKRIRTMSRRLADLSDHDRHQLRIEAKKVRYGCQFFGTMWPRTASD
                     ALVMEKTLGALQDRLGALNDLATWSRLVDLTGVTDLLPPEVDVEAELEAAQELADRIA
                     QLPGFWEATLQK"
     gene            complement(10517..14170)
                     /locus_tag="PLB86_01325"
     CDS             complement(10517..14170)
                     /locus_tag="PLB86_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63807.1"
                     /translation="MTRVSAFSLFRAEATGLPWAQIGVRALPVGQHAPDGWLDELKKR
                     TAERNNDVHVWWACSPTLGVPRGPFTVWVGSPSLKQVKDVDFTSSQRGGGLLLRWGGD
                     EAAFIVVDFDPINPNAAVGLFAHRTAATMNDVVAVSTAPAPGPVHVQLVIKCSGATSA
                     LLVNGMNAKVRIRTVEDVINDDTWKPIELVGLPCEQPWGATAYDSSDQGLVAAPVPPF
                     EAAVQRLKRGMPPLGWPPMTQTGRLAPDWVDPDPELVVKEVVQSLLPEIAELYDPGVP
                     ERLQFTLSNDRAVPGPTQDGRTSALDTSATTQPLAMLALPAQTDPVLNLATGFGTTYT
                     REGRQDGAVVGHQDFLVTADYERTPSPWPDKVTFAAYAPQTGPHEIVPPPTALTAERN
                     GLVPPNPADTPWRESIRVSWNRLPTTAAMGRVGAGTLARYDAGAAQATSLAPKRDADG
                     IRPLTLSPDGAPGTPGSGRVAFVDGAADIPILSGGRQVGYAVSLVDVHGVWSAWRDIA
                     YTGNEPGPLPPRLIALDLASTYAGSPACPATLTLETALDWTERRPTSLQIVALFFPMA
                     SASAPPPAGLDPGLPTPAGCFRRDLGLTFAGDVPTGVGATVASLGPDGANPMAPGPGQ
                     GDGGRRYRLTSGVPTLDFTSTQRWGVQVWLRSTLAVGASPTAYTPDAAHPAVTTAASP
                     VPIVPLPPPAPPGVPMGSTPDAQGSSHVRVQWSLPAGGTGVRSCIVWECSETALRQRA
                     GLPPRAPETDSPGVRLAALWTAYDGLTPTQQRAAFRRLLTLPGGARDADVTLPKGSTD
                     IHLFTVTTQSETGIESPWPGPAPTGHIHLQAATAPRLRRPTPPLTRVSVAPDGTVTVR
                     LFSASRVPVARFLVYATRSEAAARDHESMGPPVAVVPVVLPAGGPDPVTGAPVYEATW
                     TGTLPPAWDNWLVRAVAKAVDTVPVQAVRGQPSAASEVVSILVPPTGPPDLDPLVASR
                     WGADHRGVVVTSGTSGVARVTSAGVFRLGGVADAMPVAMTDLPLLPETPGTSAPAGAT
                     SSPVLERGPRVAGRLPLRLWVRTPDATAPATVSMRLLDPMGRSVERTVTVPGFVPTPA
                     YTLTIVRTSTVASGVVLAVGTDAPATVSAGISLSVRGVSTDLTPLPLPFPRPRPGLLR
                     VSGTFLLSGVRTGRITFPADGQIHVIRPQRTLGWPREARYAVFVPLLSPVRITLMLLG
                     PAGEQLVTVTGGA"
     gene            complement(14170..>16097)
                     /locus_tag="PLB86_01330"
     CDS             complement(14170..>16097)
                     /locus_tag="PLB86_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63808.1"
                     /translation="ALDTGTSLGDLAAHHATTAWAPETTGAQCRGAILRSPVDDVPEP
                     APHGSPLDRDIVKRGWADSGFKPDDLRDAVRLTPSEPALELSALLLVPTRALEGGIIL
                     RVEDPAGNEILQRPLTGADVVGTGNPLPPALTDPNGPWFDPTLRAARIGSRILADDKS
                     YAVVWVSLRKLDAEIGSVVIGWRDGAFGGKEELPFYVVAVTAVLGSEAWRESWDTTTI
                     TEDRDALQSSLTQDPDDHALLSPGTTYTVAVTWRAASVKQDSQPTATTTPDWQANETQ
                     SYRFATEPASASPTDLGPWVLASAPGMGDVGVFCTEPVRIALAHQQVADLFGAYGKEL
                     RVVVRSASGKHPAPPDGGPPGAPWTLPLGPSEFLAPALALKIGTPWSEAVATMLQTSD
                     LPCIDISVTKDHTVTITLPYTFEPLTDYLIDVHAVPVGAPASATGLVHRIPLTTSRFQ
                     SLEHFVSYIAPASVRARLVPSPAALAALPGQPTGDQVDAAYQGAGLGVPTTPSFPAVE
                     VLWSGDPVPQPVAIIVESSEPLWRSRVMPQQVTGPVDAADPLHHWWAGVTGDWLSLAP
                     STAPVAAGDLPRAGITRIVACPGRTRAVVLLGAGARGTEARLDLVLADDDLAGLPERR
                     STVVRVSLQRAPWEVED"
     assembly_gap    16098..16197
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16198..21050)
                     /locus_tag="PLB86_01335"
     CDS             complement(<16198..21050)
                     /locus_tag="PLB86_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63809.1"
                     /translation="MALWDELPSALRDSGELDGLRPLLDGLTGTGPVETTEPDGTWST
                     YTATQDLTGPLALDPRTGGFSSSSGSTGTPIEFPDPRVTVELGLHLTGPGGTMDGGWR
                     VILHAPSLRIRMPFLRGAMLDSFGHLRFDAANPIVRFTLPALRVRVQQLSGAAVGVDL
                     LSATTAGPAVDQVYEFIRMEPPYALVGPSDTVGFAFRTAVLDLSGTAGPSGVPAGARA
                     MPSNWQGLFLPDVRLFVAPEGLDGLSVMAGVENLWIGIGVHEGVTGLFEAEVVNRGHA
                     PAIVVRFVTEAGQQIGFTGDSAQLPEHTTVYAESSGGLGAYAVSITVNGVRTDDDRAP
                     VTTPPAGSISVAVALTAVGGGPVVTTRTITVSRSATPVTAGASGTPVTVRTTSSEAAY
                     LVLVEPSTPTSARVRLSSGAAATWTWPGGTITGTEAEVPVAQGATVAVTAVTTAGQVQ
                     TLDAYYLFDRPKVADGDAYAVNPANTHIGPATDRTHHSASALFIDHARTRLSVIGAAT
                     QLTVSGFASYEGDSTTAQEQRNRALSARRRDGMIAALRSVGYTNVVAGSADGTAAARA
                     GTSIDGQPAPAAGSASWWRARAVSADPPASITVTGELSRPAPTPPARTDPTPTRSQPP
                     DCFHKIGVRVELVRSTFVRCEIYGEFDIETATEASLRRRGQPALRSGPRNAADGVCTF
                     LVRLRIAEDQGAWEVSGEFRAKEGDLDGLAEMTDSNANQAALDILGALSILAPLSASA
                     TELSPAAGAVVALGSIALGASDLLHTHRLTLRGAEIIVSQGILGADGITTVSQRGTQV
                     TVLVDIEAAFSFDLTLVRVDPAHPIVTRYKAIGLRSSWDTVPAGGGGVDYVPLPVFFP
                     DRGYTLDIPAGSLTAAPPLDNILRILGVRVSRDNPTYLEVEVGLGLDLGVITIETVRV
                     RARVDGPPLDLQLTKFGASVDIPNVLTGRGYLSFETGGFSGSFDLQLIPLKLRATASL
                     AVRQAGGVTGVLLGIEVEFPVPLVLGSSGLGLFGILAGVGINHGRVEDPSAPVPALKW
                     LQDQFARPGGVMDPAGWSLQPGHYAFAAGVLLGTLEGGYIVHLKGIVLIEVPGPRLLF
                     VLKADLIKAPPALKSNQSATFLAVLDLDFGRGTITIGVVAAYEIESILTVRVPVTAFF
                     STQQVQNWFVDLGTYTDRVTVSVLDVISGSGYLMIHGNGVAIPGLPAVSNGLGIATGF
                     HISAVLMGSKAIGLYLEVAAGFDAIVGFDPFFIAGKIYARGELRLFIISIGASAELTV
                     MVGKRVVGGVVEDQPYVHGKVCGSIDLFFFEIKGCVSLTIGSEPDDDPAARPLVAGVS
                     LVSRSPALVEGSGTGRAIDGKLGDALDTTAPAAPLITVPLDAFPVITFDAAPTVGPNA
                     VMGANALGQTGAGANPWTRIGERWWRYEVLDVQLTGALIPATGGKTPSSWWTGRPPGQ
                     PVPTTALALLNWLPTPFSRAIPYGEELIRSVRDRWGTVCHPAAPSAPLLWTFADSPWG
                     PNPPGWLLDGIAWPDPPGTVRSAPAQGQLHVTEPWRIPERPDLDVLQGTEPARVIGDH
                     VPCTKRGGDTPTRAAMTRMTTSLARDLGTGPSAGSSAFGEAAYRQAMQALDTGTSLGD
                     LAAHHATT"
     gene            21386..23032
                     /gene="kdpA"
                     /locus_tag="PLB86_01340"
     CDS             21386..23032
                     /gene="kdpA"
                     /locus_tag="PLB86_01340"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRC63810.1"
                     /translation="MSPTLQAVLYVTTVALILALAHVPLGEWMYRIFTDERDWRMERL
                     VYRLVGVDPRSEQRSSVYAVSVLAFGVVSVAFLWLLILVQGWLPWGMGRGQNWHTALN
                     TAISFTTNTNWQSYAGEAGAGHTVQMVGLTVQNFVSAATGLAVAAALMRGIARQGADR
                     LGNFWVDLTRACVRLLLPIAAIAAVLLLVGGVIQNLDAPQTITTLAGQQQAVQGGPVA
                     SQEAIKLVGTNGGGFFNANSAHPFENPNGWTNLLQIVLILLIPFALPWTYGRMVGDRR
                     QGAVVTAVMAGLFAVSVTLTTWAERAAAVGVAGSLEGKEMRFGIVPSAIFGAATTSTS
                     TGAVNSMHESYSPLGGGLLMVNMQLGEVSPGGVGTGLYGMLVMVIITVFIAGLMVGRT
                     PELLGKAIGRREITYAALASVTMPALALLFTGASLLIPAARDALLNSGSHGLSEMLYA
                     YTSAANNNGSAFAGLSADQPWLNLSLGFCMFVARFGIIAFTLALAGSLARQKRRPVTE
                     GTMPTHTPLFATLLTGIVLVLAALTFFPALALGPLAEALS"
     gene            23029..25113
                     /gene="kdpB"
                     /locus_tag="PLB86_01345"
     CDS             23029..25113
                     /gene="kdpB"
                     /locus_tag="PLB86_01345"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016663117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HRC63811.1"
                     /translation="MTTTLRPHHHDGHQGPARKPLTAQALENLPQALAKLDPRHLVRQ
                     PVIFVVWIGSVLTTALSVLQPSAFAISITLWLWATIVFANLAEAIAEGRGKAQAATLR
                     QTRTTTTARRLLDDGTEEQISGNELRLGDRVVVEAGQVIPGDGDVIDGVATVDESAIT
                     GESAPVIRESGGDRCAVTGGTTVLSDRIVVQITSKPGETFIDRMIALVEGAARQKTPN
                     EIALGILLTVLTLIFVLAVIALQPMAIYSGGRLSLVVLVALLVCLIPTTIGALLSAIG
                     IAGMDRLVQRNVLAMSGRAVEAAGDVSTLLLDKTGTITFGNRRASDVLPVEGATREEL
                     LRAAYLSSLADETPEGRSIVDYALAEGLTGEVGSAADLLRDGAQFVPFTAQTRMSGVD
                     LATGTRVRKGAGSALARWVHDEGGHMAHDVDDRVDEISTAGGTPLVVAIQEPGTAPRQ
                     LGVIFLKDIVKPGMAERFAQLRQMGIRTVMITGDNPLTAAAIAKEAGVDDFLAEATPE
                     DKMALIKAEQEGGRLVAMTGDGTNDAPALAQADVGVAMNTGTSAAKEAGNMVDLDSDP
                     TKLIDIVGIGKQLLITRGALTTFSIANDIAKYFAIIPAMFVSTFPGLAALNVMRLHSP
                     QSAMLSAVIFNALVIVALIPLSLKGVAYRPLSAAALLRRNLIIYGLGGVLTPFLGIKA
                     IDLLVSLVPGLS"
     gene            25119..25685
                     /gene="kdpC"
                     /locus_tag="PLB86_01350"
     CDS             25119..25685
                     /gene="kdpC"
                     /locus_tag="PLB86_01350"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HRC63812.1"
                     /translation="MTRQLWAATRALLVLTVLLGVAYPLGMTALALAMPDRATGSLVM
                     IDGRPAGSVLLGQATVEPQWFRARPSVSDYSGETSGGSNWGPSAAELSADLAARAADL
                     RAVNPLAPAGPIPADALTTSASGLDPHISPAYAAWQAPRVAAARGMTVEQVQRLIDAN
                     TTSGELGFLGADRVNVTTLNAALATLGR"
     gene            25709..28210
                     /locus_tag="PLB86_01355"
     CDS             25709..28210
                     /locus_tag="PLB86_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC63813.1"
                     /translation="MARGRLRIYLGAAPGVGKTVAMLSEGARRKARGTDVVVGVVETH
                     DRAHTAAMLDGLEIVPRRTVPHRGATITEMDLPAVLARHPQVVLVDELAHTNAPGSEH
                     HKRWQDIEALLAAGIDVISTVNIQHLESLNDAVEDITGIVQQERVPDAVVRAADQIEL
                     VDMSPEALRRRMAHGNVYKSDKVDAALSHYFRVGNLAALRELALLWLADRVDEGLDLY
                     RHAHDIDATWPTRERIVVAVSGGPESEALVRRGARIAARGAGGELVVLYVARADGLAG
                     APVEALAKVRRMVEEVGGTFHTISGEDRARAILQFARAENASTILVGASRRPWWRALV
                     RPGVAAPVIEGSGDIDVHVVTHSAAGTRHAYVARAPLARRRVVEGYAWGVVGGAVLTA
                     VLWQLDARLGQPLGLPLVVQLYLVHTIVASLIGGMAPAIVTAVISSLTVNWFFTPPIG
                     NLTIAAPENALALALFIVVAAAVSLVVHRSSRRAETALAAQQESAALAELTHTLLGST
                     EQLALLLSRAIDMFGVTGAAVVRRRTKDEPDSVIAATDGFDLSGGATTTEAVDEEHDL
                     ILTGGPVPAERKRLLAAYSAHAGAILARRALQDQARSAEHLTRDNKARTALLSAVSHD
                     LRTPLAGIKAAIGSLRSHEVEFSPEDRAELEEAIDDSADRLESLIGNLLDMSRLQVGA
                     LVAHPRLTDLGEIVPAAIIGSAATGRVTWTLGEGARYAEADPGLLDRVLSNLVENAMR
                     HHDDPAKIRVTTSRIRDEVQLRVIDSGPGIPEAMREPAFQPFQRHTDSGGSGVGLGLA
                     VARGLAEAMGGTLTAEETPGGGLTMVVALPAGVSP"
     gene            28207..28899
                     /locus_tag="PLB86_01360"
     CDS             28207..28899
                     /locus_tag="PLB86_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC63814.1"
                     /translation="MSARVLVVDDDPTILRTLRINLRARNYEVEAVTNGRDALSAFVE
                     SPPDLVVLDLGLPDVDGVEVLRRMRRISRVPVVVLSARQDSDDKVEALDEGADDYVTK
                     PFGMDELMARIRAAIRRGGEALASTPPFSCDDFTLDFDERTCEASGSIVHLTPTEWRL
                     LAELARHDGRIVPHENLLKAAWGPTYGRESNYLRVYVSQLRRKLEPDSSRPRHLLTEP
                     GLGYRLVRRGHD"
     gene            complement(28933..29808)
                     /locus_tag="PLB86_01365"
     CDS             complement(28933..29808)
                     /locus_tag="PLB86_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRC63815.1"
                     /translation="MIRALEVVATGPLALVEDLGRLGYAAVGVGRSGAADRAAYLLAG
                     RLLGEPVGRAAVEVTLGGLVVRARGSLTVVVTGAAAPATVDGRPVGHAAPVHLPDGAV
                     LALGMPPTGLRSYLAVRGGIDLDPVLGSRSSDTLSGIGPAPLRVGQVVPVGEPGAGGP
                     TMDVAPVAAPEGGRVELTLLPGPRLDWLADPRALTATPWTVSGSSDRVGMRLTGPALS
                     RAERWADAELPSEGVVVGGVQVPADGQPIVFLADHPVTGGYPVVAVVRAADVDRAAQC
                     RPGQDVRLRWAVAPR"
     gene            complement(29805..30515)
                     /locus_tag="PLB86_01370"
     CDS             complement(29805..30515)
                     /locus_tag="PLB86_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HRC63816.1"
                     /translation="MPSETEPGLVRVLPCGETALLVEVADTLTAVALARQFDHLRAAG
                     GPPWSDVHDVVTGERTVLVIAGHLTTMTELRPAILESVSAARLPVLKLGGELVTGGNR
                     TPAIVAPLEIPVVYDGADLLDVAALTGLSPAQVVDAHTGADWDVGFTGFAPGFAYLVG
                     GDPRLVVPRRAEPRPRVAAGSVALAGPYSGIYPTDSPGGWQLIGRTEVTLWDLHRDPP
                     AVLRPGMRVRFRNVGGPP"
     gene            complement(30499..31275)
                     /locus_tag="PLB86_01375"
     CDS             complement(30499..31275)
                     /locus_tag="PLB86_01375"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HRC63817.1"
                     /translation="MSDNRVVHIDLNADLGESFGAWTMGDDLALLDVVTSANIACGFH
                     AGDPLTMTRTVGAALDRGVTVGAHVSYRDLVGFGRRFIDASEEEVRADVLVQLAALDG
                     VCRAAGGSVTYLKPHGALYHAAARHEPHARAIVAAVAAYNGSLVILGMAGSRLLTLAS
                     EAELPTATEAFADRGYRADGTLVPRGEPGDLLHDPDEIAARVVGMARDGAVPSVEGTA
                     VPISADSVCVHGDTPGAVAIARAVRAALEQAGVAVRAFGD"
     gene            31468..33723
                     /locus_tag="PLB86_01380"
     CDS             31468..33723
                     /locus_tag="PLB86_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC63818.1"
                     /translation="MSASLAARRWTHRAFGGAVALALAGSAAMTAQAATGSAAIDPNS
                     DGVVATSGVDSSSALIQLVGDPLSTSPDVDHGNGRVKLDGQKTKSVKAALAAQRNALK
                     SWLRANAPKAKVTGEYDFALNAIAVRLNGTSVATLRQAPGVASVQLQGLFHPTADDPD
                     LARIDGLLGWSVAGATSEPSDPSSWAGAGVQVGIIDSGVDASHPCFSDAGFPATRQLG
                     DTHFTNNKVIVAKVFNNKAGSKGYTAEAIGEHGTHVGGTVACNLQTPAVVNGVDIPYD
                     PSGVAPGAQLGSYNVFPGDDGNARSEDILNAIEAAVADGMDVLNMSLGGGSHGIQDLL
                     TVAVDNVDRAGVVVAVSAGNEGPGVMTIGSPGMAERALTSGASTVGHFVGLSITSDVG
                     EGTEVYAATGDFPVPDAPITATLAAVKTAQGAFGIGCSAADVPPALVSGKIALISRGT
                     CTFGTKVANAKAAGAVAVIIVNNVAGDPIAMVADTALSAQAQALPAVMTALSAKGALL
                     ALDGELVTIGSDQAYRITDSNDIMAGFSSQGPVDVSYRVKPDVVAPGVNVLSSIPMSY
                     CDNDVEGCWAFFQGTSMSSPHLAGMAAVVRAANPGWDAWQVRSAIANTAKLGVLTSFT
                     DGTTVDVNVQKVGNGLADLDAAVRAQVALTRPSVSFGAVPNTAGQSLTETVTVTNLTD
                     GALTLPLSVSEGPGEGVFSVSPQSVNLPAGGSATVTVTFTSAKGSALGNTQATLMVGS
                     AAHAALYAYLK"
     gene            complement(33872..35053)
                     /gene="hutI"
                     /locus_tag="PLB86_01385"
     CDS             complement(33872..35053)
                     /gene="hutI"
                     /locus_tag="PLB86_01385"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRC63819.1"
                     /translation="MTATLITGIAELVTNDSSWSAAGQAPDVARLGIVTDAAVLVEAG
                     EVVWVGPASAAPQADSRVDVGGCAVMPGFVDSHTHLVFAGDRAAEFAARMAGTAYDGG
                     GIATTVAATRGATDDELRTLLAARIAELRRQGTTTVEIKSGYGLSVEDELRLLRLARE
                     VTPHVTFLGAHVVPAEYAADRAAYLDLVTGPMLAAVVAQGAATYVDVFCEPLSAHAFT
                     EDEARVILRAGRAAGLAVRVHGNQLGPGPGVRLACELGAVSVDHCTYLSDADVAALAE
                     TGTVATLLPGVEFSTRHPYPDARRLLDAGVAVALATDCNPGTCYTSSMPFVIALAVRE
                     LRMTPAEALYAATAGSARTLGLRGRIAVGEPADLTVLDAPSYLHLAYRPGVPLASTLK
                     V"
     gene            complement(35050..36381)
                     /locus_tag="PLB86_01390"
     CDS             complement(35050..36381)
                     /locus_tag="PLB86_01390"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HRC63820.1"
                     /translation="MTSYWCEYAWLPGGLAASVRLEMMGERIVGVSADVEARPEDVRL
                     AGVTLPGLANTHSHAFHRALRGRTHGDGGTFWTWRERMYAVASRLDPARYLALARAAY
                     AEMALAGFTAVGEFHYVHHQPDGTPYADPNAMGAALIQAAAEAGIRLTLLDTIYLAGG
                     LGPDGHLPLDVVQQRFSDDSVDAWSTRVGLMSDTPTARMGAAIHSVRAVPKDALAGVV
                     AASVGRPLHVHLSEQPAENATALAFYGVTPTALLEGAGALGPMTTAVHATHLTDDDIA
                     SLASAGVTACFCPTTERDLADGIGPARALADAGVPLALGSDQHAVVDPFEEARGLESH
                     ERLVTLERGRFTPGQLTDALSRNGYAALGWPDGGRLAAGALADFVTVRLGSPRTAGTA
                     PEQILYAATGADVTTVVVGGEVVVSAGQHRLGDVGRLLTDAISALDDGAAS"
     gene            complement(36386..37645)
                     /locus_tag="PLB86_01395"
     CDS             complement(36386..37645)
                     /locus_tag="PLB86_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HRC63821.1"
                     /translation="MAPFSTPDGAAARSFDRMWADLAPIGRNPATGGYERFAWTREDH
                     TLREWFAGECGARGLEVVRDRAGNQWAWWGDPDARPGIVLGSHLDSVADGGAFDGPLG
                     VVSALAALDALRDNGFTPRMPLGIANFADEEGGRFGVACAGSRILTGALSPDRARSLT
                     DGDGVTMAQAWTAAGRDPATLGPDPDALRRVAAYVEVHVEQGRALCVDPDDPFGDPAL
                     WDPARAVAIGTEIWPHGRWQIDVFGEANHAGTTALADRNDAMLGFASVVASARMLARE
                     RGCVATVGKVIARPNAVNAIADHVTTWLDARGADPEAVRGLVQTLRDEVSEDGTTVDE
                     VSWTPATVFDTRLRDRLAAVLPEAPLLATGAGHDAGILANAGIPTAMLFVRNPTGVSH
                     SPQEFAERDDCIRGIHALAAAAAELAG"
BASE COUNT         5650 a        13109 c        13560 g         5418 t
ORIGIN      
        1 cggcatactg gggaactgtc ctccacgaga gaaagccatc catgaccagc gcccctcact
       61 ccagtgccga cttcatcgag ctggagcacc gctttgcggc ccacaactac tctccgctgg
      121 acgtcgtcat cgcccgtggc gaaggggcct gggtggagga cgtcgaaggg cgccgctact
      181 tcgacgcgtt ggcgggttac tcggcgctca acttcgggca ctccaacccg cggctcatcg
      241 aggtcgccga gcggcaactg cgcacgctga cgctcaccag ccgggccttc cactccgacc
      301 agctcggccc gttcgcccgc gacctgtgcg cgctgaccgg caaggacctc atcctcccga
      361 tgaactccgg cgccgaggcc gtcgagaccg cgatcaaggt gacccggcgg tggggcaagc
      421 ggaccaaggg cctgccggag gactggggca cgatcatcgt catgggcggc aacttccatg
      481 gccgcacgac gacgatcgtc tcgttctcga ccgacaaggt cgcccgcgag gactacggcc
      541 cctatacccc gggcttcctc gtcgtcccct atggcgacat cgacgcgctg cgcgccgcga
      601 tgaccgacga gaccatcggt gtcctgctcg agccgatcca gggcgagggc ggggtggtca
      661 tcccgccgga gggcttcctg cgtgaggtgc gcgcgctgtg caccgagcgc aacgtcctca
      721 tggtcgccga cgagatccag tcgggtctcg cgcgcaccgg tcgcaccttt gcctgcgatc
      781 acgaggacgt cgttcccgac atctacatct tgggcaaggc gctcggtggc ggcatcgtcc
      841 cgctgtcggc catcgccgcc aacaccgatg tgctcagcgt catcaccccg ggctcgcacg
      901 gctcgacttt cggcggcaac cccttcgccg cggcgatcgg ccgcgaggtc atcgcgatgc
      961 tgcagaccgg cgagttccag gagcgcgctc gcctcctggg tgacctcctg gccggcgagc
     1021 tcgccgcctt gaaccacccg ggcatcgtcg aggttcgcct gcgcggtctg tgggcgggca
     1081 tcgacgtcga tccggccctg atgagcggca aggagttgtg ccaccggatg gctgaccggg
     1141 gcgtgctcgc caaagacacc cacggctcga cgatccgtct ggcgccgcca ttggtcgcca
     1201 ccgaggccga catccgcatg ctcgtctcgg tgatcgccga ctccctcaac gcctgaccgg
     1261 accccgccga aaggtgggat tcagtgggta tgccgtccgc cgaggccacc gtttgccacc
     1321 tttcgcggag ggtcaggcct gcttacccgg ctgacaccag gcgcagggct cgatgcgggc
     1381 acgcagtggc agcggccttc gccaagccca gcaggtcggg ggtcacggtc ccttcaacga
     1441 cgggaaaacc ccactcgtcc aagtggatcc gctcaggcag cagctcggcg cacaagcccc
     1501 gggcggcgca cgacggccaa tcgacgcgca tcgtccacgt catcgcgagc accccccacg
     1561 caggtgcagg gcgacctcgt catcaaacgt ggtcagcagt gatcgcacca accgggctgt
     1621 cccgtccgga tgggcgcacg cgccgcgttt gtcgaccagg cccaccaact ctccgatccg
     1681 ggtccacgaa gcgctggttt cgcccagggc cacgagccgc atctcctcgg caagggcagg
     1741 caggccgttg aggcagggcc cgcaccgtcg agcggaggct ttggcgaggt agtcgacgat
     1801 ttcactggtg accgccaccg ggcactgatc cgcccgcatg ggcaggacca cccctgcgcc
     1861 caacccgagt ccacgggcgc ggagttcctc gcggctgacc gtcgcctcgg cgaggacacc
     1921 cgcgtcggcc cacatgccgt ggaaaccgcc cagcaggacg ggttgctcga cgtccggtcc
     1981 cagcacgtcc acccacggcg tcccgtatgc cgcctccagc accaccgcgg tgtcggcgtc
     2041 gcgccgatgc gggagggtga ggagagtcgt gccgggctct gtatcggtgc cgtgggcggc
     2101 ataccccagg gctcccatcc gcaggacggc ggcgacctgc gcccaggtct cggcgttgga
     2161 cagcagggtg gggcgcccgt cgagacccga catggcctcg ggcttccatg ccgtcaccgg
     2221 caggccgggc cgaccggcga ggagttccaa gacggcccgc gcctggccag acacgaagcc
     2281 cggttcggcg gcccgcatcc gccagcgcgg ctggctccgg tcgcggcggc cgggctgggc
     2341 cgcgcgctcg gaaagggcgg cagcgatggc gtcgccgacc gcgggtcgct ccagcggcgt
     2401 gacgatgtga acctcggtgg tgcgcagtgc gcgcgccacc acctcggccc cgtcgaggat
     2461 ctggtgaggc gccacgaggg cgagcgccgt gtccttcgcg gaggccggct cgccttcggc
     2521 gaggttgatg accacgaccg ctcggcgccg cccggtcgcc gcggcggcca acttacgggc
     2581 gaaggggaaa ccggcgccgc cccggccgac gacggcgccg gcgccgaccg tggccaagac
     2641 cgtgtcgagg tcgagctgag ggagctggcc aaaccggtcg cggtgtgcct ccagcgacgg
     2701 tccgtccagc acgccggaca ggagcaccgg ccctggggtg gcgacgacct gagcaggcgg
     2761 atccgtgcgg accgtgcgtg gcaccgaccg agcgctgagc gaccggcgca cggtcgccgc
     2821 aggtgccgag ggtgcaacgg gggctccggc gcccgcgcca gggaaggagg tgaccgaggc
     2881 caggaccgcc ccgctcatga ccgaaccacc tggacgcggc gggccacggc ccgaccggcg
     2941 acggcaacga cgacggctgc cacacagctc agcacggtcc agcgtcccca cggcgacccg
     3001 gcgtcggtgc cgatcccgag ggtgtggatc accgcgacac cccaactcac gtaagccaac
     3061 aggtggatgc gccgccacac ccgctcggag atccgcgagc gcagcaggct gctcacgccg
     3121 atggccagca gcaggtccag gctcagcgcc cccatcccga ggtagagcgg gcgatacagc
     3181 ccccctacgg gcaggaatgc gtcgaaccac cggatgtcga cgtaggtgtc tacgacggcc
     3241 gtcgtcacgt gggcggcgag caggacggtg gccaacagcg cgaggttgcg gtgcagcccc
     3301 tgggtgacga atcgggggac gaaccgaccg ggcccgcgac cggttgccga caggatgccg
     3361 agcagcacga ccgccgtata gagaacgagc aggacgatcc cggtgcccct gttggcgaac
     3421 cacaggagcg gaccggacag gtcgatggac ggaaggctca cgtgcgggac tcctcaaggt
     3481 ctgtgggcca ggtaggggtg cggcggaccg acccgtcggc cgcgagcaag cgtgcggcga
     3541 cgccgtgttg ggccaaccat tcgggggcgt cggcccccag gacgagcgcg gcggtcgtcg
     3601 cggtgttcgc atcggccgcg cagtccccgt atgccgtgac tgacagccag ggggtctgcg
     3661 ccggtcgacc ggtgcgcggg tccaggacgt ggtgccaggc cgatccggcc cgaacccacc
     3721 gtcgggcggt gactgatgag gtggccagcc cgcccgcggt cagggacacg cggcatacct
     3781 cggggtcgtt gtccgaccac gcgcgcaggt cgggcgcgag gtcgaccggc caggtgactg
     3841 cggcgccggc cggggcgtgc gcgcggacgt ctccgccgac gctgacgacg acagccacgc
     3901 cgagccgttc gacgatggtg agggcgacga ggtcggcggc atacgccttg ccggtggcgc
     3961 cgaggtcgag ggcgacgccg tgcgggacgc gcacccggtc accgtcgatg cggacgtccg
     4021 accaggcgcc ggcgaccggc acgggcaggg cgtgggcctc gacggtgctg gggagttggc
     4081 tgaaggtgcg gtcatagccc gcggcgacca tgagctctcc gagggtggga tcgaccagcc
     4141 cgtcggtcga gtgggcggct cgaagcgcca cccggaccgc gccgatgagg atcgggctga
     4201 ccgacaccca gtggccgcca gcctgattgg cccggaccag gtcactgtcc tggcggaatc
     4261 gactgcaggc cacgtcgacc tcggacagca ctgcggcggc aagcgccgca gcctcgtcgg
     4321 cgacggtcgc gtcggccacg ccaaggtgga cgtaggttcc cagtgctcgc cacgagcgcg
     4381 agcccgggtc ggtcgggacg tcgtatgccg tccccccagc cccccgcctt acgcgggatt
     4441 cggcgggggg ctggggagtt gcggcgcgca gggcgagggt cacgaccctg atgagacgac
     4501 gggggcgggt ttcggcgccg gcttgggcgc ggccggcttc ggagccacga ccggcttcgg
     4561 ggctgcgacc ggtttcgggg ctgcgaccgg tttggccacc gtggtcgtcc tgctcgcgct
     4621 cgtcttcggt ttcggcgaag ggctgagcga gccgccggtg gcggctgctg tggtcaccga
     4681 ttcgctgacc acggtgcggt aggtgcggtc cacgatttcc gccgccgtct gctgtgcggc
     4741 tgccgccttt gcctgggcct tgagcgcctg ctgctgcgcg gtgtccgccg ccgccagacc
     4801 ggtgatggcg ccggtcgagg caacggtggc ggcagcgacc acgccggtga gggcgtgcat
     4861 ggcggtcatg gaccggtcgc gagaggcggg caaccaagtt cgattcatgt ggttcatcgt
     4921 cgacgcggct gcggcaaggg cgcgtcaggc gcacctcaag acaacgctaa gaagtccggt
     4981 cagcggtagc gcctggaggc ctggacggcg gatgcgcggc cgaattctta gcactccctt
     5041 gatgctgccc gagggtgcgg ttgacccaag gtgagtcacc gtgaaggtgc accgcgttcc
     5101 cacgccgacg tcgatggcgc ccgcggtccc tgcacctcgc acatcgaatc ggaaggcaac
     5161 ctcatgtgga tcggatggaa ggccatcagc ctcggagtcg tcagtggggg ggcgatcgcc
     5221 gtcgcgctct atagctcgct ggccgcgggt tcggccgccc catcggcgtc gatcccgatc
     5281 tcgcagaccg tcccgaccgc ggtctatgcc gactgcgtgg agccggcggt tctcgagggc
     5341 gatgagtgcg tcacccacgt gagcgtcacc cgcacggtgg ccgcgcctgc cccggcggcc
     5401 gttcccgtcc cggcggttgc tgcgtcatca gcgaccacgt ccgcgacccg gtacacgccg
     5461 gccccgaccc cggaggcagc gccggcggcc gcggcggtcg acgaccacga cgatgacgag
     5521 catgaggacg agcgcgacgg tgacgacgag cctgacgacg acgagcgcga cggtgacgac
     5581 gactgaccaa cccgcaccgg gccggagcaa gcttgcgacg cagtgggatt cagtcggcgc
     5641 gctcgatgag gggcaggacg acgatcacgg cgaggccgcc aagcccggcc cgttcgaaac
     5701 cgaccgaccc tcccgcgtca gccgccaggc ggcggacgat gtccagcccc aacccggtgc
     5761 tcccggcgcc actctcaccg cgcgcgaacg caaccgtgtc ggggctgccg tccccgccgt
     5821 cctcgacccg caggaccacc agcccatcga cgacgtgcaa ctccaaccgg attggcgtct
     5881 cctcaggcgt gtgcgcgaac gcgttgtcga cgaggttgtc cagcaggtcg gccaggtccc
     5941 gagccgccag cccgaccaac gccggtcggt atgccgcgtg ctcacctccc gggtgctcac
     6001 ccgtgacgct ctctcccgcc gccccgctgg ccgaaccgtt cgccgagccg ccgatcacgg
     6061 tggtcatgcg cccctggtcc tcggccagcg gaccccagtg atgggcccgg tcggcgagga
     6121 cgactcgggc gtcgcaccat ccgcccattg cgtcgcggct cgggcgccgg gcgtcggcga
     6181 ccaccgagtc gatggcgcgg tgcaggtcct cgacatggct gcgcaaccgg gcccgaaccg
     6241 tcgggtcctc cacctgatcg gtgtccagcc gcagtgccgt gaccggagtg cgcagccggt
     6301 gggacaggtc ggcggcgccc tcgcgctcga tccgcagcag gtcctcgatc cggtccgcga
     6361 gttgattgag ggcatgtcca agctcgacga cctcgaccgg ccccgtggcc gctgctcggg
     6421 cttcgaagtc gccggatcgc agccggtggg cgacaccagc catcgcgacg accggtgtgc
     6481 tgatccgggc gctctgacgc cgggcgacga gcacgctcac cacgacgagc aacaggccga
     6541 ccgcgacgag gatcgcccac gagccgtaaa ccccgcgccg cacccgtccg aggtcggtga
     6601 ccgagtgcac gatcgcgacc ccgtcggcga caccgacggg gatcagcacg tggaagcggt
     6661 catcgacggt cgtcgccatc gaccggttgt cggcccggac cgcggcgagg aggcgggcat
     6721 cctccgggaa ctgcgccggg ccggacgagg gctgaccggt ggtgacaggg ccggtggttg
     6781 tcgggcctcc aataagttga ccggagggga gagcgacggt cgtcgtcgcc gtgcgggcac
     6841 cgagggtggg ggccaggact gccgggagcg acgccgggtc gtcgaccccg gcgacgatca
     6901 tcgccacgct ctgggcgtca ctgttggtcg cggagaccgc tcgctcctcg gccatggtcc
     6961 gcacgagcag gcacaggggg atgacgaacg agatcacgac gccggcgacc aacgccgcgg
     7021 ccagccacgt gatccgagac ctcagcatca gtcagccgac ccggggtggg ccccgcgcgg
     7081 gtcgaccaac cgcacaccga ccccccgaac gctatggaga tagaccggat cggcggcggt
     7141 ctcgccgagc ttgcgccgca gccaggacag gtggacatcg accgtccggt cgccgccgcc
     7201 ataactctgc cgccacacct cggtgaggat ctcgcgcttg gtgacgacct cgccgacccg
     7261 ctgggccagc aggtgcagca ggtcgaactc cttgcgggac aactccacgg gtatcccgtc
     7321 caccgtcacc tcgcgactgg ccgaggtgat gaccagaccg ccgacgacga tgggtgtcgg
     7381 cggcgcgtcg tgccccgccc ggcgcaagac cgcacggatg cgtgcctcga gttggtcacc
     7441 gccaaacggc ttgacgaggt agtcgtcggc gccgaggtcg agggctcgaa cgatctcctt
     7501 ctcgtcgtcg cgagccgttg cgacgaccac aggcaccgag ctcacggcgc gcaacatcga
     7561 cagcacggtc aggccgtcga cgtccggcaa cccgaggtcg agcacgacga catccggcgg
     7621 ctggtcgacg gcctcggtca gcccggccat gccggtcggc tggctggtca ccgcgtggcc
     7681 cgcgccggcc agactgcgca acaacgcgcc acggatcgcg ggatcatctt cgacgacgag
     7741 cacgcgagcc atgggggtta cgctacgcgc agcatgggga ggaaccgatg agcggatcgc
     7801 gggcacaact gctgcggtat gccgcaacct gggtgctcgt ggtcagcgtg acggctgggc
     7861 tgtcgtgggg cgcgatctct cgggccggag aggcggccac actcctcggc gcgccggtcc
     7921 cactcggctc ggcggcggcc ccgacgaccg ccggcttgac gcccagcgcg acccagtcgg
     7981 tcccgccccc gccgccggtg acgtcggcac cgacctccag cgggcagtcg tcgacctccc
     8041 cgagctcccc aagcaccccg aagcccgagc cgaccgagtc gccccgtccg gtggtccggt
     8101 cagtcagcac ggcggccggt caggtctggg cctactgcgt cggcgacacc gcgtaccgcc
     8161 gcagcgtcgt ggcgaccgac ggctggagct tcgagttctc gcgcgaagac aacgggcgct
     8221 tcgaggtgaa gttccaactc gcgggagcgg aggaggaaga ggtcgaggtg aaggttcgct
     8281 gtgaggcggg tctcccggtt ttcaccaccg attgagcgag ccgcggccgc ttgaggtgag
     8341 cgggcggcat accccgtggg ggctaggcgt attcccagcg gaggccgacg atcccgcggc
     8401 gagcgcgctc gcgcaccagg gtgactcgac ggtccggtga gagcgtccgt tggcggacga
     8461 acaggtcggg gccgttgtcg gtggcccgct cgaactcctc gaggcgcacc ggcagacagt
     8521 cggggtggaa gcccaggtcg aggaccatca tcgagcgcga ggtgacgacg agccgataga
     8581 gcaggaggct gcgctgctcg ttgtcatcga acgtgaggta gtgcaggaaa tggtgctcac
     8641 cgcggcggac gaagcgatcg aagacgagtt cgctgacgac gacgttggcc tccgcatccc
     8701 ggcggacccg cccgatgcgt ccgccgctga ccgcctcgtg gcggatgagc tcaggtcgcc
     8761 cgcccggttc cccgccgtgc aggatcggga accgatcggt gtcgaccagg gcgcgatagg
     8821 tctcgaccgc gcgcaccgac cgcaggcacc ccgtgggtcc gaactcggcg acctggatgg
     8881 cggacaacgg ttcgagggtg gccatgccgt cggggcagcc gacgccctcc atgaggacgc
     8941 cgattcgggc agccgcggcg gccatgtcga gctctgggct gaccggggtc gagttcgggt
     9001 ggcgcaaggc ctcggtgaaa ctgccgattg gcaccttgag gatttcctcg agggcggtca
     9061 cggcgaggag cgaggtcggc tgggtgggca ggcggtgacc gttctgccag tagctcaacg
     9121 tgctgcgacc gatcgcgaaa ccgcgctcgg ccaaacgacg ttggacagca tccaaggtga
     9181 ggccgctcgc gtggagcgcc acccgcaact cccccgagaa gcggttcgat ccggtgtcga
     9241 tcaccaccag agattcacag tccttccgtg aacgttcaca cccagggcga tcgtggcacg
     9301 gggagcgggc gttcacaagc gggcgcgttc acatacccga caatccatat cgaaccagcc
     9361 ttgtccgacg tgtccgggcc ggtgagaatt caagcactgt tgggaagcag tgacgttgat
     9421 cgggaccggc ccaaatcgga cccccgaaag acatccaccg cgctgtcggc tgatgcggga
     9481 ccgcggcgcg aacgatggcc ggacccctcc tggtgtcagg ggtccggcca tcggcccatc
     9541 actcgccata ctctgcaacg cccttcacgt ctggcgcccc tcacttctga agcgtggcct
     9601 cccagaagcc gggcagttgc gcgatccgat ccgccaactc ctgcgcggcc tccaactcgg
     9661 cctcgacgtc cacctcgggc ggcagcagat cagtcacccc cgtgaggtcg accagtcggc
     9721 tccacgtcgc gagatcgttg agcgcgccga gacgatcctg cagggcaccg agggtcttct
     9781 ccatgacgag ggcatcggag gcagtccgcg gccacatcgt cccgaagaac tgacagccgt
     9841 agcgaacctt cttcgcctcg atccgcagct ggtggcggtc gtggtcggag aggtcggcca
     9901 gtcgcctgct catcgtgcgg atccgcttgc ggcgtcgttc gagggcgccg gccgtccacg
     9961 ctcggccgct ccacgtgcca gtgccgagca ggtcgcggta ggacccgttc cgcagccact
    10021 cgtcgaggtc gactcgggtc tgcgcccact cgggcgagtg caggaactcc agtgcctcgc
    10081 tgtatgccgt aacccttgcg tcttccaggc gcgctgcgtt ggtggggctg aggtccgggt
    10141 gcccttcgag gaagacgtcg aggtcgcgag ccgggccgaa cgcgacggtg agctcacgca
    10201 gctgggcctt gagccgccgt gcttcgggat cggcccggct gacctcgcgc gcgagggaga
    10261 acagcgaccg cagccgccga tagccaaccc ggaactggtg tagcgcttcg tgatcgccat
    10321 gggcgtccag gagtggttcg ttggcgtcgg tgtgctcaag gcagcgcctc gccactgatt
    10381 cgagggtgcg accgcccccg agccgtggcg aaagcacgat cgaaggcgcc ttcgtcgggg
    10441 tcgtcatgcc cgacatcctg tcagttcgcc tgctgaccga cgtgtttggg ccgcgacgac
    10501 cgtcggtgcc gccggctcag gcaccgcccg tgacggtcac caactgctcg cccgccgggc
    10561 ctagcagcat cagggtgatc cggacgggac tgagaagcgg gacgaacacg gcatacctcg
    10621 cctcccgcgg ccaccccagg gtccgctgcg gacggatgac atggatctgg ccgtccgcag
    10681 ggaaggtgat tcgccccgtg cgcacccctg agaggaggaa ggtccccgac actcgcagga
    10741 gtcctggcct cggtctgggg aacggcagcg ggaggggggt gaggtccgtc gacaccccgc
    10801 ggacggacag gctgatcccc gcgctcacgg ttgccggcgc gtcggtgccg acggcgagga
    10861 ccacgccgga cgccaccgtc gaggtgcgca cgattgtcag cgtgtatgcc ggtgtcggca
    10921 cgaatccggg caccgtcacc gtccgctcga ccgagcgacc catcgggtcg agcagccgca
    10981 tcgagaccgt cgccggggcg gtggcgtcgg gggtgcgaac ccacagccgc agtggcaacc
    11041 gacccgcaac gcgtggccct cgctccagga caggggaact cgtcgcaccg gcaggtgcgc
    11101 tggtgccggg agtctccggc aggagcggca ggtcggtcat tgccaccggc atcgcgtcgg
    11161 cgaccccgcc gaggcggaag acgcctgcgc tggtcacccg ggccactccc gaggtgccgg
    11221 acgtgacgac gactccacgg tgatcggccc cccagcgaga ggcgacgagg gggtcgaggt
    11281 cgggaggtcc ggtcggcggg acgaggatcg agacgacctc ggaggccgcc gacggctggc
    11341 cgcggaccgc ctggaccggc acggtgtcga ccgccttggc gacggcgcgc acgagccagt
    11401 tgtcccacgc gggcggcagc gtccccgtcc aggtcgcttc gtagaccggt gccccggtca
    11461 ccgggtccgg tccgccggct gggagcacga cgggcacgac ggcgaccggt ggccccatgc
    11521 tttcgtggtc gcgggcggcg gcctcggagc gtgtggcata gacgaggaag cgggcgaccg
    11581 gcacccggct ggccgagaag agccgcacgg tgacggtgcc gtcgggcgcc acgctcaccc
    11641 gggtcagcgg cggcgtcggc cgacgcaacc gaggggccgt ggccgcctgc aggtggatgt
    11701 ggccggtggg cgccggcccc ggccagggtg actcgatccc ggtctcggac tgggtggtga
    11761 cggtgaagag gtggatgtcg gtcgacccct tgggcagggt gacatcggca tcgcgtgcgc
    11821 cgcccggcag cgtgagcaac cggcggaatg cagcccgttg ctgggtcggg gtcagcccgt
    11881 cgtaggcagt ccacaacgcc gctaggcgca cgcccggcga gtcggtctcg ggcgctcgcg
    11941 gcggaagtcc ggcgcgctgg cgcagcgccg tctcggagca ctcccagacg atgcaactgc
    12001 gcacgccggt gccgccggcg ggcagtgacc actgcacgcg cacatgcgaa ctcccttgtg
    12061 cgtcaggggt gctgcccatg gggacgcccg gcggagccgg cggcgggagc ggcacgatcg
    12121 gaacggggct cgcggctgtc gtcaccgcgg ggtgcgccgc gtcaggggtg tatgccgtgg
    12181 ggctggctcc cacggccaac gtgctgcgca gccagacctg gaccccccac cgctgggtgc
    12241 tggtgaagtc aagggtcggc acgccgctgg tcagtcggta gcgccgcccg ccgtcgccct
    12301 ggccggggcc gggcgccatg gggttggctc cgtccgggcc gagcgaggcg acggtggcac
    12361 cgacccccgt cggcacgtcg cccgcgaagg tgaggccgag atcgcgccgg aagcacccgg
    12421 ccggggtcgg caggccgggg tcgagccccg ccggtggcgg cgcgctcgcc gaggccatgg
    12481 ggaagaacag ggcgacgatc tgcaggctcg tgggtcggcg ttcggtccag tcgagtgccg
    12541 tctccagggt gagggtcgcc gggcaagcgg ggctaccggc atacgtggag gccaggtcga
    12601 gggcgatgag gcgagggggc aacggtcccg gctcgttgcc ggtgtaggcg atgtctcgcc
    12661 acgccgacca gacgccgtgg acgtcgacga gcgacacggc ataccccacc tggcgcccgc
    12721 cgctgaggat cgggatgtcg gccgcgccgt cgacgaaggc gacccgcccg gagccgggag
    12781 tccccggcgc gccatcgggg ctgagggtca gcgggcggat gccgtcggcg tcgcgcttgg
    12841 gagcgaggga cgtggcctgg gcggccccgg cgtcgtagcg agccagcgtt ccggcgccga
    12901 cccgccccat cgcggcggtc gtcggaaggc ggttccacga cacccggatg ctctcgcgcc
    12961 acggggtgtc cgcggggttg ggcggcacga ggccgttgcg ctctgcggtg agcgccgtcg
    13021 ggggcggcac gatctcgtgc ggcccggtct gcggggcgta tgccgcgaac gtcaccttgt
    13081 cgggccacgg gctcggggtg cgttcgtagt cggcggtgac gaggaagtcc tgatggccga
    13141 cgacggcccc gtcctgcctg ccctctcggg tgtaggtcgt gccgaaaccg gtcgccaagt
    13201 tgagcaccgg gtcggtctgg gcaggcaggg cgagcattgc caacggctgg gtcgtcgccg
    13261 aggtgtcgag ggcagacgtg cgcccgtcct gcgtcggccc cgggaccgct cggtcgttcg
    13321 agagcgtgaa ctgcaggcgc tctggcacgc ccgggtcata gagctcggcg atctccggca
    13381 gcagcgactg gacaacctcc ttgacgacga gctcggggtc ggggtcgacc cagtccgggg
    13441 cgaggcggcc ggtctgggtc atcggtggcc agccgagggg cggcataccc cgcttgagcc
    13501 gctggaccgc cgcctcgaag ggaggcacgg gggcggcgac gagcccctga tccgagctgt
    13561 cgtatgccgt tgcgccccag ggttgctcgc acggcaggcc gacgagctcg atcggcttcc
    13621 aggtgtcgtc gttgatgacg tcctcgacgg tgcggatccg gaccttggcg ttcatgccgt
    13681 tgacgagcag cgccgaggtg gccccggagc acttgatgac gagttggacg tgcacgggtc
    13741 caggcgcggg cgctgtgctc accgcgacga cgtcgttcat cgtcgccgcg gtgcggtggg
    13801 cgaagagacc gacggcggcg ttggggttga tcgggtcgaa gtcgacgacg atgaaggccg
    13861 cctcgtcccc tccccagcgc agcagcagcc cgccgccgcg ttgggacgag gtgaagtcga
    13921 catccttcac ctgcttgagc gaggggctac cgacccagac ggtgaacggc ccgcgaggga
    13981 ccccgagggt gggcgagcac gcccaccaca cgtggacgtc gttgttgcgt tcggcggtgc
    14041 gcttcttgag ttcgtctaac cagccgtcgg gcgcgtgctg cccgaccggc agagcgcgca
    14101 ctccgatctg ggcccacggc aacccagttg cttccgcgcg gaacagcgag aaggcgctca
    14161 ccctggtcat cagtcctcca cctcccaggg ggctcgctgc agtgagaccc ggacgacggt
    14221 gctccgtcgt tcgggaaggc ccgcgaggtc gtcgtcggcg agcacgaggt ccagtcgggc
    14281 ctcggtgcct cgcgcgccgg cgccgagcag gacgacggct cgggtgcgtc cggggcaagc
    14341 gacgatccga gtgatcccgg ctcttggcag gtcacctgcg gcgaccggcg cggtgctagg
    14401 agccagcgac agccagtcgc ccgtgacccc ggcccaccag tggtgcagtg ggtcggcggc
    14461 gtcgaccgga ccggtgacct gctggggcat gacccgcgag cgccagagtg gttcgctgct
    14521 ctccacgatg atcgccacgg gctgcgggac ggggtcgccg ctccacaaca cctcgaccgc
    14581 ggggaacgac ggcgtcgtcg ggacaccaag cccagcacct tggtatgccg cgtcgacctg
    14641 atcgccggtc ggctgtccgg gcagggcggc gagggccgcc gggctgggca ccagccgggc
    14701 tcgcaccgag gccggcgcga tgtagctgac gaagtgctcc agcgactgga accgtgaggt
    14761 cgtgagcggg atgcggtgga ccaggccggt ggccgacgcg ggggcgccga ccggcacggc
    14821 atggacgtcg atgaggtagt cggtgagcgg ctcgaaggtg tagggcaagg tgatcgtcac
    14881 cgtgtggtcc ttggtgaccg agatgtcgat gcacggcagg tcggacgtct ggagcatcgt
    14941 cgccacggcc tcgctccacg gggtcccgat cttgagagcc aaggccggag cgaggaactc
    15001 gctgggcccg agaggcagcg tccacggggc gccgggcggg ccgccgtcgg gtggcgccgg
    15061 gtgtttgccc gaggccgagc gcacgacgac acgcaactcc ttgccgtagg ccccgaacag
    15121 gtcggcgacc tgctggtggg ccagggcgat ccgcaccggt tcggtgcaga acactccgac
    15181 gtcgcccatc ccgggcgcgc tggcgagcac ccatggcccg aggtcggtcg gtgacgcgct
    15241 ggccggctcg gtggcgaacc ggtaggactg ggtctcgttg gcctgccagt ccggcgtggt
    15301 ggttgctgtc ggctgggagt cctgtttgac cgaggcggcc cgccaggtca cggcgaccgt
    15361 gtaggtcgtt ccggggctca gcagggcgtg gtcgtcgggg tcctgggtga gggagctctg
    15421 cagggcgtcg cggtcctcgg tgatggtcgt cgtgtcccac gactcccgcc aggcctcgct
    15481 gccgaggact gcggtgaccg cgacgacgta gaacggcagt tcctccttgc cgccgaaagc
    15541 accgtcgcgc cagccgatga cgaccgagcc gatctcggcg tcgagtttgc gcagcgagac
    15601 ccacacgacg gcatacgact tgtcgtcggc gaggatgcgc gagccgatcc gggccgctcg
    15661 cagggtcggg tcgaaccagg gaccgttggg gtcggtgagc gcggggggca gggggttgcc
    15721 ggtgccgacg acatcggcgc cggtgagggg gcgctggaga atctcgttgc ccgccgggtc
    15781 ctcgacgcgc aggatgatgc cgccttcgag ggctcgggtg ggaacgagca gaagcgccga
    15841 caactcgagg gctggctcgg agggggtgag gcggacggcg tcgcgcaggt catcgggttt
    15901 gaatccgctg tcggcccaac ctctcttgac gatgtcgcgg tccagcggcg acccgtgggg
    15961 ggccggctcg gggacgtcgt cgacgggcga gcgcaggatc gcaccgcgac actgcgcgcc
    16021 ggtggtctcg ggggcccatg ccgtcgtggc gtggtgggcg gccaggtctc cgagggacgt
    16081 tccggtgtcc aacgcctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc
    16201 gtggcgtggt gggcggccag gtctccgagg gacgttccgg tgtccaacgc ctgcatggcc
    16261 tgccggtatg ccgcctcgcc gaacgccgag gaccccgcgc tcgggccggt gcccagatcc
    16321 ctggccagcg aggtggtcat ccgggtcatg gccgcgcggg tcggcgtgtc gccgccgcgt
    16381 ttcgtgcacg gcacgtggtc gccgatgacc cgcgcgggct cggtgccttg aagcacgtcg
    16441 aggtcggggc gctcggggat ccgccagggt tcggtgacgt ggagctggcc ctgggccggc
    16501 gccgatcgca ccgtcccggg agggtcgggc caagcgatgc cgtccaggag ccacccaggg
    16561 ggattgggtc cccagggcga gtccgcgaag gtccacagca ggggggccga cggtgcggcc
    16621 gggtggcaga cggtgcccca gcggtcccgg accgagcgga tgagttcttc gccgtaaggg
    16681 atggcgcggg agaagggggt tggcagccag ttgagcaggg cgagcgcggt cgtcgggacg
    16741 ggctgccccg gagggcgtcc ggtccaccag gagctcgggg tcttgccccc ggtggcgggg
    16801 atgagcgcgc cggtgagctg gacgtcgagc acctcataac gccaccaacg ctcgccgatg
    16861 cgggtccatg ggttggcccc ggcgccggtc tgtcccaacg cgtttgcccc catgacagcg
    16921 ttcgggccca cggtgggtgc ggcgtcgaag gtgatgaccg ggaaggcgtc cagcggcacg
    16981 gtgatcagtg gcgcggccgg ggcggtcgtg tcgagggcgt cgcccagttt gccgtcgatg
    17041 gcccgaccgg tgccggaccc ctcgacgagg gcggggctgc gcgagaccag gctgaccccg
    17101 gcgaccaacg gccgggcggc cgggtcgtcg tcgggctcag acccgatggt gagtgagaca
    17161 cagcccttga tctcgaagaa gaacaggtcg atcgagccgc aaaccttgcc gtggacgtag
    17221 ggctggtcct ccacgacacc acccacgacc cgcttgccca ccatgacggt gagctcggcc
    17281 gacgcgccga tcgagatgat gaacaggcgc agctcgccgc gggcatagat cttgccggcg
    17341 atgaagaacg ggtcgaaacc gacgatcgcg tcgaaccccg cggcgacctc gagatagagc
    17401 ccgatcgcct tgctgcccat gaggacggcc gagatgtgga agccggtggc gatgcccagc
    17461 ccgttggaga cggccggcag ccccgggatg gcgaccccgt tgccgtggat catgaggtag
    17521 ccgctgccac tgatgacatc gagcaccgag acggtgaccc gatcggtgta ggtcccgagg
    17581 tcgacgaacc agttctgcac ctgctgggtg gagaagaacg cggtcaccgg cacccggacg
    17641 gtgaggatcg actcgatctc gtatgccgcg acgacaccga tggtgatcgt gcctcgcccg
    17701 aagtccaggt cgaggacggc gaggaaggtc gcgctctggt tggacttgag tgccggcggg
    17761 gccttgatga ggtcggcctt gaggacgaag agcagtcgtg gcccgggcac ctcgatgagg
    17821 acgatgccct tgaggtggac gatgtagccg ccttcaagcg tcccgaggag caccccggca
    17881 gcgaaggcgt agtggccggg ctgcaacgac caccccgccg gatccatgac gccgccgggt
    17941 cgggcgaact ggtcctgcaa ccacttgagc gcaggcaccg gagcactcgg gtcctcgacc
    18001 cgcccgtggt tgattccgac gccggcaagg atgccgaaga gcccgagccc tgaactgccc
    18061 agcacgagcg gcacggggaa ctcgacctcg atgcccagca gcacgccggt gacgccgccg
    18121 gcctgccgca cggcgaggct ggcggtggcg cgcagcttga gcgggatgag ttgcagatcg
    18181 aacgaccccg agaagccgcc cgtctcgaag gacaggtaac cgcgcccggt gaggacgttg
    18241 gggatgtcga cgctggcacc gaacttggtg agctgcaggt ccagcggcgg accgtcgacc
    18301 cgcgcgcgga cccggacggt ctcgatggtg atgacaccta ggtccagccc cagaccgacc
    18361 tcgacctcga ggtaggtcgg gttgtcgcgg ctgacccgca cgccgaggat ccgcaggatg
    18421 ttgtccagcg gcggggcggc ggtgaggctg cccgccggga tgtccagggt gtagccccgg
    18481 tcggggaaga acacgggcag ggggacgtag tcgaccccgc ccccacccgc aggcacggtg
    18541 tcccacgagg agcgcagacc gatggccttg tagcgggtga cgatcgggtg ggccgggtcg
    18601 acccgcacga gggtgaggtc gaaggagaac gccgcctcga tgtcgacgag caccgtcacc
    18661 tgggtgcccc gctgggacac cgtggtgatc ccgtcggcgc cgaggatgcc ctgcgacacg
    18721 atgatctcgg ccccgcgcag ggtcagccgg tgggtgtgca gcagatcgga ggccccaagc
    18781 gcgatcgagc ccaacgcgac gaccgccccg gccgccggtg acagttcagt ggcgctcgcg
    18841 ctgagcggcg cgaggatcga cagggccccg aggatgtcga gggccgcttg gttggcgttg
    18901 ctgtcggtca tctcggccaa gccgtcgagg tcgccctcct tggctcggaa ctcgccgctg
    18961 acctcccacg ctccctggtc ctcggcgatc cgcagtcgga cgaggaaggt gcacacgccg
    19021 tccgcagcat tgcgtggacc ggagcgcagc gccggttggc cgcggcggcg cagcgacgcc
    19081 tcggtggccg tctcgatgtc gaactcgccg tagatctcgc agcgcacgaa cgtgctgcgc
    19141 acgagctcga cgcggacgcc gatcttgtgg aagcagtccg gtggctgcga tcgggtcggc
    19201 gtcgggtcgg tgcgggctgg cggggtgggc gcgggtcggc tcaactcacc ggtgacggtg
    19261 atcgaggcgg gcgggtcggc gctgaccgcc cgcgcgcgcc accaggacgc ggagcctgcg
    19321 gccggcgcgg gctggccatc gatcgatgtc cccgcccgag cagccgcggt gccgtcggcg
    19381 ctgccggcga cgacgttggt gtagcccacc gagcgcaagg cggcgatcat gccgtcccgc
    19441 cgtcgggccg acagggcccg gttgcgttgc tcctgagcgg tggtggagtc cccttcgtag
    19501 gacgcaaaac cgctgaccgt cagctgcgtc gcggcaccga tgaccgacag gcgcgtccgc
    19561 gcgtggtcga tgaacagggc gctcgccgag tggtgggtcc ggtcggtcgc ggggccgatg
    19621 tgcgtgttgg cggggttgac ggcatacgca tcgccgtcgg cgaccttggg gcggtcgaag
    19681 aggtagtagg cgtcgagggt ctgcacctgt cccgccgtgg tgacggcggt gaccgcaacg
    19741 gtcgccccct gcgcgaccgg cacctccgcc tcggtcccgg tgatcgtgcc gcccggccag
    19801 gtccaggtcg ccgccgcccc tgacgagagg cgcacccgcg cgctcgtcgg ggtgctcggc
    19861 tcgaccagca cgaggtatgc cgcctcgctc gaggtcgtcc gcacggtcac cggagtgccg
    19921 ctcgcgcccg ccgtcaccgg cgtcgcgctg cggctcacgg tgatggtgcg cgtcgtgacg
    19981 acgggtccac cgccgaccgc ggtgagggcg acggcgaccg agatcgaccc ggcgggtggc
    20041 gtggtgacgg gcgcgcggtc gtcgtcggtg cggacgccgt tgacggtgat cgacacggcg
    20101 taggcgccga gcccgccgga cgactcggca tacaccgtgg tgtgctcggg gagttgcgcg
    20161 gagtctccgg tgaagccgat ctgttggccc gcttcggtga cgaatcgcac gacgatcgcg
    20221 ggggcgtggc cgcggttgac gacctcggcc tcgaagagcc cggtgacgcc ttcatggacg
    20281 ccaatgccga tccagaggtt ctccacgccg gccatgaccg agaggccgtc gagcccttcg
    20341 ggcgcgacga acaggcggac gtcgggcagg aagaggccct gccagttgct gggcatcgcg
    20401 cgggcgccgg cgggcacgcc gctcgggccc gcggtcccgg acaggtcgag gaccgccgtg
    20461 cggaaggcga acccgacggt gtcggacggg ccgacgaggg cgtagggcgg ttccatccgg
    20521 atgaattcat agacctggtc gaccgcaggg ccggccgtgg tcgccgagag caggtcgacg
    20581 ccgacggctg cgccggagag ttgctgcacc cgaacccgca acgcgggcag ggtgaaccgg
    20641 acgatcgggt tggccgcgtc gaagcgcagg tgcccgaagg aatcgagcat cgcgccgcgc
    20701 aggaacggca tccggatccg cagggacggg gcgtgcagga tgacccgcca tccgccgtcc
    20761 atggtgccgc cgggaccggt gaggtgcagg cccaactcga ccgtcacccg ggggtccggg
    20821 aactcgatcg gggtgcccgt cgagccgctg gagctgctga agccgcccgt ccgcgggtcg
    20881 agggctaacg gcccggtgag gtcctgggtc gcggtatagg tcgaccacgt cccgtcgggt
    20941 tcggtggtct cgaccgggcc ggtgccggtc agtccgtcga gcagcggacg tagaccgtca
    21001 agctcgcctg agtctcggag cgccgaaggc agctcgtccc acaacgccat ggcacttcct
    21061 tgggagagtt ggaagttcga gcgtggacaa cgccggggcg tcgtgggtcc tagtggagca
    21121 cagaccagtg cgaggcgcgc gccgaatgcc cggattgcct ctcgatttcc tctcgcagcc
    21181 ctctcggccg ggtcgacccg ttgccagtgg ggccgtcaag atcgcgtcaa gaagcgctag
    21241 gagctccttg ctgtcggcgg agcgccggat ccggtcggat gcacttgggg gcatggcaga
    21301 agacatcgtc gggctcgtca tcggcttgag cctcgtcggc tatttgctct acgcgctcgc
    21361 gcgccccgag aggttctgag tcgaggtgag ccccaccctg caggccgtcc tctatgtgac
    21421 gacggtggcc ctcatcctcg ctctggcgca cgtgccgctc ggcgagtgga tgtatcggat
    21481 cttcaccgat gaacgcgact ggaggatgga acgcctcgtc taccgactcg tcggtgtcga
    21541 tccgcgctcc gaacagcgct cgtcggtcta cgcggtgagc gtgctggcct tcggcgtggt
    21601 gtcggtggcc ttcctctggc tgctcatcct cgtccagggc tggctcccgt ggggcatggg
    21661 ccgcggccag aactggcaca ccgccctcaa cacggcgatc tcgttcacga ccaacaccaa
    21721 ctggcagtcg tatgccggtg aggctggtgc cggccacacc gtccagatgg tgggtctcac
    21781 cgtccagaac ttcgtgtcgg ccgccaccgg cctcgccgtc gccgcggcgc tcatgcgcgg
    21841 cattgccagg cagggcgccg accgactcgg caacttctgg gtcgacctga ctcgggcctg
    21901 cgtgcggctg ctcctgccga tcgcggcgat cgccgccgtg ctcttgctcg tcggtggcgt
    21961 catccagaat cttgatgccc cgcagacgat cacgaccctc gccggtcagc agcaggccgt
    22021 ccagggcggc cccgtcgcca gccaagaggc gatcaagctc gttggcacca acggcggcgg
    22081 cttcttcaac gccaactcgg cgcacccctt cgagaatccg aacgggtgga ccaatctcct
    22141 ccagatcgtc ctcatcctgt tgatcccctt cgccctgcca tggacctacg gccggatggt
    22201 cggcgaccgt cgccagggcg ctgtcgtcac agcggtgatg gctggattgt tcgcggtctc
    22261 ggtcaccctc acgacgtggg ccgagagggc cgccgcagtc ggcgtcgcgg gctcgttgga
    22321 gggcaaggag atgcgattcg ggatcgtgcc ctcggccatc tttggggcgg cgacgaccag
    22381 cacctcgacg ggtgcggtca actcgatgca cgagtcgtac agcccgctcg ggggtggcct
    22441 gctcatggtc aacatgcagt tgggcgaggt gtcccccggt ggggtcggca cggggctcta
    22501 cgggatgctc gtcatggtca tcatcaccgt gttcatcgcc ggtctgatgg tcggccgcac
    22561 gcccgaactc ctcggcaagg cgatcggccg ccgcgagatc acgtatgccg cgctcgcctc
    22621 ggtgacgatg ccggccctcg ccctgctctt caccggtgcg tcgctgctga tcccggccgc
    22681 ccgagacgcc ctgctcaaca gcgggtcgca cgggctgtcg gagatgctct acgcctacac
    22741 ctcggcggcc aacaacaacg gctcggcgtt cgcaggcctc tcggccgacc agccctggct
    22801 caacctctca ctggggttct gcatgttcgt cgcgcggttc gggatcatcg ccttcaccct
    22861 ggcgctggca ggcagcctcg cccggcagaa gcgccgcccc gtgacggagg gcacgatgcc
    22921 cacccacact cccttgttcg cgaccctgct gaccggcatc gtcctcgtcc tcgccgcgtt
    22981 gaccttcttc ccggccctgg cgcttggccc tctggcggag gctctctcat gacgaccacg
    23041 cttcgaccgc accaccacga cggacaccag gggccggccc gcaagccgct cactgcccag
    23101 gccttggaga acctgcccca ggcactggcc aaactcgacc ctcgacacct ggtccgtcag
    23161 ccggtgatct ttgtcgtttg gatcggctcg gttctgacga cggcgctgtc cgtgctgcag
    23221 cccagcgcct tcgcgatctc catcaccctg tggctgtggg cgacgatcgt cttcgccaac
    23281 ctcgcggagg caatcgctga gggccgaggc aaagcgcaag cggcgactct ccgccagacc
    23341 cgcaccacga ccacggcccg gcgactgctc gatgacggga cggaggagca gatctctggc
    23401 aacgagcttc ggctgggcga ccgagtcgtc gtcgaggcgg gccaggtgat ccctggagac
    23461 ggcgacgtca tcgacggcgt cgcgaccgtc gacgagtcgg ccatcaccgg cgagtccgcc
    23521 cccgtgatcc gcgaatccgg cggcgaccgc tgcgcggtca cgggcggcac gacggtgctg
    23581 tcggaccgga tcgtcgtcca gatcacgagc aagccgggcg agaccttcat cgaccggatg
    23641 atcgcgctgg tggaaggcgc agcgaggcag aagacgccga acgagatcgc cctcgggatc
    23701 ctgttgaccg tcctcacgtt gatcttcgtc ctggcggtca tcgccctcca accgatggcc
    23761 atttactccg gcgggcgcct gtcgctcgtt gtgctcgtcg ccctcctcgt ctgcctcatc
    23821 cccaccacca tcggtgcgct gctgtcggcc atcggcatcg ccgggatgga ccggctggtc
    23881 caacgcaacg tgttggccat gtccggccgg gccgtcgagg cggcaggtga cgtgtcgacc
    23941 ctgctgctgg acaagacggg gacgatcacc ttcggcaatc gccgggcctc tgacgtcctt
    24001 ccggtcgaag gggcgacgcg tgaggaactg ttgcgcgcgg catacctctc ctcgctggcc
    24061 gacgagacgc ctgagggacg gtcgatcgtc gattacgcgc tcgcggaggg cctcacgggc
    24121 gaagtgggtt ccgccgccga tctcctgcgg gacggcgccc agttcgttcc gttcacggcg
    24181 cagacccgca tgtctggggt cgacctcgct acgggaacgc gcgtgcgcaa gggcgccggg
    24241 agcgcgttgg cccggtgggt gcatgacgag ggtggccaca tggcacacga tgtcgatgac
    24301 cgggtcgacg agatcagcac tgcgggtgga accccgctgg tcgttgccat ccaggagccg
    24361 gggactgctc cccggcaact cggcgtgatc ttcctcaagg acatcgtcaa gccaggcatg
    24421 gccgagcggt tcgcgcagtt gcgtcagatg ggcatccgca cggtgatgat caccggcgac
    24481 aaccccctca ccgccgcggc gatcgccaag gaggccgggg tagacgactt tctcgccgag
    24541 gccacgcccg aggacaagat ggccctcatc aaggctgagc aggagggcgg ccggctcgtc
    24601 gcgatgaccg gcgacggcac gaatgacgct ccggcgcttg cgcaggccga cgtcggcgtg
    24661 gcgatgaaca ccggaacctc ggcggccaag gaagccggaa acatggtgga cctcgattcg
    24721 gatccgacca agctcatcga catcgtcggt atcggcaagc agttgctgat cacccgcgga
    24781 gcgttgacca ccttctcgat cgccaatgac atcgctaagt acttcgcgat catccccgcc
    24841 atgttcgtca gcaccttccc cgggctcgcg gcgctcaacg tcatgcggct gcactccccg
    24901 cagtcagcga tgctgtccgc ggtcatcttc aacgcgttgg tcatcgtcgc cctgatcccc
    24961 ttgtcgctca agggtgtggc ctatcgaccg ctcagcgcgg cggccctgct gcgacgcaac
    25021 ctgatcatct acggccttgg cggcgtcctg acgccgttcc ttggcatcaa ggccatcgac
    25081 ctgctcgttt ccctcgtccc cggattgtcg tgacagccat gaccagacag ttgtgggctg
    25141 cgacccgcgc cctcctcgtc ctcaccgtcc tcctcggtgt cgcctacccg ctgggcatga
    25201 ccgccctcgc cttggcgatg cctgatcggg ctaccggttc gctcgtcatg attgacggtc
    25261 ggcccgcggg ttcggtgctg ctgggtcagg ccacggtcga accgcagtgg ttccgggccc
    25321 gaccgtcggt gtcggactac tcgggggaga ccagcggcgg cagcaactgg ggcccgtccg
    25381 ctgccgagtt gtcagccgat cttgcggcgc gcgcagccga cctccgcgcg gtcaacccgt
    25441 tggcgcccgc cggtccgatc cccgctgacg ccctcaccac cagcgccagc ggcttggacc
    25501 cgcatatctc cccggcgtat gccgcatggc aggcgcccag ggtcgccgcc gcccgcggga
    25561 tgaccgttga gcaggtgcaa cgactgatcg acgcgaacac gacgtccggg gagctcggct
    25621 tcctcggcgc tgatcgcgtc aacgtcacca ccctcaacgc ggccctcgcc accctcgggc
    25681 gctgacggca tacgggacaa tcgacaccat ggcacgcggc cgactgcgga tctacctcgg
    25741 ggctgccccc ggggtcggta agacggtcgc catgctttcg gagggtgccc gacgcaaggc
    25801 tcgtggcacg gacgtcgttg tcggggtcgt cgagactcac gaccgagctc acaccgcggc
    25861 gatgctcgac ggccttgaga tcgtgccgcg ccgcacggtc ccgcaccgcg gggcgacgat
    25921 caccgagatg gacctccctg cggtgctggc ccgtcacccg caggtggtgc tggtcgacga
    25981 actcgcccac accaacgctc ccggatctga gcaccacaag cgctggcagg acatcgaggc
    26041 gttactggcg gccggcattg acgtgatctc gacggtgaac atccagcacc tggagtcgct
    26101 caacgacgcc gtcgaggaca tcactgggat cgtccagcag gagcgggtgc ccgacgccgt
    26161 cgtccgagcc gccgaccaga tcgaactcgt cgatatgtcg cccgaagcac tgcgccgtcg
    26221 gatggcccac ggcaacgtct acaaatctga caaggtcgac gccgccctgt cgcactactt
    26281 ccgggtcggc aacctcgccg ccttgcggga gctcgcgctc ctctggcttg ccgaccgggt
    26341 cgacgagggg ctggacctct atcgacatgc ccacgacatc gacgccacct ggcccacccg
    26401 cgaacggatc gtcgtcgcgg tctccggcgg cccggaatcc gaggccctcg tccgacgggg
    26461 agcccggatt gcagcgcgcg gagcaggtgg tgagctcgtc gtcctttacg tcgcgcgagc
    26521 cgacgggctg gctggcgcgc ctgtcgaagc cctcgccaaa gtgcgacgca tggtcgagga
    26581 ggtcggcggc acgttccaca cgatctccgg cgaagaccgc gcgcgcgcca tcctccagtt
    26641 cgctcgcgcc gagaacgcct cgacgatcct cgtcggtgcc agccgccggc catggtggcg
    26701 ggcgctcgtc cgacctggcg ttgcggcccc ggtcatcgag ggatctggtg acatcgacgt
    26761 ccacgtcgtc acgcatagcg ccgccggcac ccggcacgcg tacgtcgcgc gggcgccgct
    26821 ggcccgccga cgcgtggtcg agggctatgc ctggggggtg gtcggcgggg ccgtcctcac
    26881 ggccgtccta tggcagctcg atgctcgact cggccagccc ctggggctgc ccctcgttgt
    26941 ccagctctac ctcgtgcaca ccatcgttgc gtcgctgatc ggtgggatgg ccccggcgat
    27001 cgtcaccgcc gtcatctcct cactcaccgt gaactggttc ttcaccccgc cgatcgggaa
    27061 cttgacgatc gcggcgcccg agaacgccct ggccttggcc ctgttcatcg ttgtggcggc
    27121 agccgtatcg ctggtcgtgc accgcagttc ccgccgggcg gagacggcgc tggccgcgca
    27181 gcaggagtct gccgccctcg cggagttgac ccacacgttg ttgggatcga ccgagcaact
    27241 cgcgctcctg ctgagccgcg cgatcgacat gttcggtgtc accggggcag ccgtcgtccg
    27301 ccgccggacg aaggacgagc cggactcggt catcgcggcc accgatgggt tcgacctcag
    27361 cggcggcgcc acgaccaccg aggccgtgga cgaggagcac gacctcatcc tcaccggagg
    27421 gccggtgcct gccgagcgca agcggctgct cgcggcatac tccgcccacg ccggagccat
    27481 cctcgcgcgc cgcgccctac aggatcaggc gcgcagcgcg gagcacctga cccgggacaa
    27541 caaggcccgg accgcgctgc tgtccgcggt gtcccacgac ctgcgcaccc cgttggcagg
    27601 gatcaaggcg gctatcggca gcctgcgcag tcatgaagtc gagttcagcc ccgaggaccg
    27661 agccgagctc gaggaggcga tcgacgactc ggccgaccgg ctggagtctc tgatcgggaa
    27721 cctcctggat atgtcccgcc tccaggtcgg cgcgctcgtg gcgcatccgc ggctcaccga
    27781 cctcggcgag atcgtgccgg ccgcgatcat cgggtcggcc gccaccggac gggtcacctg
    27841 gaccctgggc gagggagcga ggtatgccga ggccgacccc ggcctcctgg accgggtgct
    27901 cagcaacctt gtcgagaacg ccatgcgtca ccatgacgac ccagcgaaga tccgggtgac
    27961 aaccagtcgg atacgggacg aggtgcaact tcgagtgatc gactcgggcc cggggattcc
    28021 cgaggcgatg cgcgagccgg ccttccaacc tttccaacgg cataccgact ctggggggag
    28081 cggcgtcggt ctcgggctgg ctgtcgcgcg cgggctggcc gaagcgatgg gcgggaccct
    28141 caccgcggag gaaactcccg gcggtggcct caccatggtc gtcgccctcc cggctggggt
    28201 gtccccgtga gcgcccgagt gctcgtcgtc gacgacgatc cgaccatcct gcggacgctg
    28261 aggatcaacc ttcgcgcgcg caactacgag gtcgaagcgg tcaccaacgg tcgcgacgcc
    28321 ttgagcgcct tcgtggagtc cccacccgac ctcgtcgtgc tggacctggg gttaccagac
    28381 gttgatggcg tcgaggtgct gcgacgaatg cggcggatca gccgggtgcc ggttgtcgtc
    28441 ctgtccgccc ggcaggacag cgatgacaag gtggaagccc tggacgaggg tgccgacgac
    28501 tacgtgacca agcccttcgg gatggatgag ctgatggccc ggatccgcgc cgcgatccgc
    28561 cgcggcggtg aggcgctggc ctccactcca ccgttctcgt gcgatgactt caccctcgac
    28621 ttcgacgaac ggacctgcga ggcaagcgga tccatcgtgc acctcacccc gaccgagtgg
    28681 cgcctgctgg ccgagttggc ccgccacgac ggacgcatcg tcccccacga gaacctcctc
    28741 aaggctgcct ggggacccac ctacggacgg gaatccaact acctacgcgt ctacgtgagc
    28801 caactccggc gcaagttgga gcccgactca tcccggcccc gccacctgct gaccgagccc
    28861 ggtctcggtt accgcctcgt gcgacgaggc cacgactgac ggcggttgcg tcggtggggc
    28921 aggctgcggg attcagcggg gggcgactgc ccaacgcagc cgcacgtcct ggccggggcg
    28981 gcactgggct gctcgatcca catcggcggc ccggacgacc gcgacgacgg ggtagccgcc
    29041 ggtgaccggg tggtcggcga ggaacacgat cggctgtccg tcggcgggca cctggacgcc
    29101 gcccacgacg actccctcgc tgggcagttc ggcgtccgcc cagcgctcgg cccggctcag
    29161 cgccggcccg gtcaggcgca ttccgacccg atcactgctc cccgacaccg tccatggcgt
    29221 ggccgtcaga gcccgtggat cggcgagcca gtccaggcga ggtccaggca gcagggtgag
    29281 ctcgaccctc cctccctcgg gtgccgcgac aggggcgacg tccatggtgg gtcctcccgc
    29341 cccgggctcg ccgacgggca ccacctgacc cacccgaagc ggcgccgggc cgatccccga
    29401 cagggtgtcg ctggaccgcg acccgaggac agggtccaga tcgatccccc cgcggacggc
    29461 gaggtagctg cgcagcccgg tgggcggcat acccaacgcg aggacggccc cgtcggggag
    29521 gtgaacgggc gctgcgtgac cgaccggtcg gccgtcgacc gtggcaggag cggccgcgcc
    29581 ggtgacgacg acggtgagcg agccgcgggc ccgcacgacg aggccgccga gggtgacttc
    29641 cacggcggcg cgcccgaccg gctccccgag cagacgcccc gcgaggaggt atgccgcccg
    29701 gtcggccgcc ccggaacgcc ccactcccac agcggcatac ccgagtcgtc cgaggtcttc
    29761 gacgagggcg agcggcccgg tggcaaccac ctcaagcgcg cggatcacgg cgggccgccc
    29821 acgttgcgaa agcgcacccg catccccggc cgaagcaccg cgggagggtc tcgatgcaga
    29881 tcccagaggg tcacctcggt gcgcccgatg agctgccaac cgccgggtga gtcggtgggg
    29941 tagatcccgc tgtaggggcc ggcgagggcg accgaaccgg cggcgacgcg cgggcgaggc
    30001 tcggcccggc gcgggacgac gagcctcggg tcgccgccga cgagataggc gaagccgggg
    30061 gcgaacccgg tgaaccccac gtcccagtcg gcccccgtgt gcgcgtcgac cacctgtgcg
    30121 gggctcagcc cggtgagagc tgccacgtcc agcagatccg ccccgtcgta gacgacgggg
    30181 atctcgaggg gggcgacgat cgcgggcgtc cggttgccgc cagtcacgag ctcgccccca
    30241 agcttgagca cgggaagcct tgctgcactc acggattcga ggatcgccgg tcgcagttcg
    30301 gtcatcgtgg tcaagtgccc ggcgatgacg aggacggtgc gctcgccggt gaccacgtcg
    30361 tggacgtcgc tccaaggcgg cccgccggca gcccgcaagt gatcgaactg tcgggccagg
    30421 gccaccgcgg tcagggtgtc ggcgacctcg acgagcagcg ccgtctcgcc acacggcagg
    30481 acgcggacga gccccggctc agtctccgaa ggcacgaacg gccaccccgg cctgctccag
    30541 ggcagcgcgg accgcgcgtg cgatggcaac ggccccggga gtgtcaccgt ggacgcacac
    30601 cgagtcggcc gagatcggca cggcggttcc ctcgaccgag ggcacggcgc cgtcgcgagc
    30661 catgccgacg acgcgggcag cgatctcgtc ggggtcatgc agcaggtcgc ccggctcacc
    30721 gcgaggcacg agggtgccgt cggcgcggta accccggtcg gcgaacgcct cagtcgccgt
    30781 gggcaactcg gcctcggagg cgagtgtgag gagtcggctg cccgccatcc cgaggatgac
    30841 caaggagccg ttgtatgccg cgaccgcggc gacgatcgcc cgcgcgtgcg gctcgtggcg
    30901 ggcggcagcg tggtagagcg cgccgtgggg cttgaggtag gtgacgctcc caccggcggc
    30961 acggcatacc ccgtcgaggg ccgcgagctg gacgaggacg tcggcgcgga cctcctcctc
    31021 gctggcgtcg atgaagcggc gaccgaagcc gaccaggtcg cgataggaca cgtgggcgcc
    31081 cacggtgaca ccccggtcga gggccgcccc gacggtgcga gtcatggtga gggggtcgcc
    31141 ggcgtggaag ccgcaggcga tgttggcact ggtgacgacg tccaacaagg ccaggtcgtc
    31201 gcccatcgtc caggcaccaa aggactcgcc caggtcggcg ttgaggtcga tgtgcacgac
    31261 acgattgtcc gacatggacc ggtgtggggc agcggcctcg cgaccaaccg gcccttggcc
    31321 gcaggattcg ttgctcaggt gcctgttgac cggagcttat gcgggctagc attcccccag
    31381 cggctcgggg cgcgcgctgc atgcttctgc gcgtggcccc ggtgggagcg gacgctcgac
    31441 ccggactcaa ggacgagagg atccctcgtg agtgcatcac tggctgcgcg tcgctggacg
    31501 catcgagcgt tcggaggcgc ggtcgccctg gccctggcgg ggagtgcggc gatgaccgcg
    31561 caggccgcga ccggctcggc cgcgatcgac ccgaactcag acggcgtcgt cgcgacctca
    31621 ggggtggact cgtccagcgc cctgatccaa ctcgtgggcg acccgctctc gacctcgccc
    31681 gatgtcgacc acggcaacgg ccgggtcaag ctcgacgggc agaagaccaa gagcgtcaag
    31741 gcggcgctcg ccgcccagcg caacgccctc aagtcgtggc tgcgcgccaa cgcccccaag
    31801 gcgaaggtga ccggcgaata cgacttcgcc ctcaacgcga tcgcggtgcg ccttaacggc
    31861 acgtcggtcg ccacgctgcg tcaggcgccc ggcgtggctt cggtgcagtt gcaggggctc
    31921 ttccacccga cggcggacga cccggacctg gcccgcatcg acggcctgct cggttggtcg
    31981 gttgccggcg ccacctcgga gcccagtgac ccgtcatcgt gggccggcgc gggcgtgcag
    32041 gtcggcatca tcgactccgg tgtcgatgcg agccacccat gcttcagtga cgcaggcttc
    32101 cctgccaccc ggcaactcgg cgacacgcac ttcaccaaca acaaggtcat cgtcgccaag
    32161 gtcttcaaca acaaggccgg cagcaagggg tacaccgctg aggcgatcgg cgagcacggc
    32221 acccacgtcg gtggcacggt cgcctgcaac ctgcagaccc cggccgtggt caatggcgtc
    32281 gacatcccct atgaccccag cggcgtggcc cccggggccc agctcggtag ctacaacgtc
    32341 ttcccgggtg acgatggaaa cgctcgcagc gaggacatcc tcaacgctat cgaggccgcg
    32401 gttgccgacg ggatggacgt cctcaacatg tccctcggtg gtggctcgca cggcatccag
    32461 gacctgctga ccgtcgccgt cgacaacgtt gaccgagcgg gcgtcgtcgt cgccgtctcg
    32521 gcgggcaacg aaggccccgg cgtcatgacc atcggctccc cgggcatggc cgagcgagcg
    32581 ctcacctcgg gcgcctcgac cgtcgggcac ttcgtcggcc tctccatcac ttccgatgta
    32641 ggcgagggca ccgaggtgta tgcggcgact ggcgacttcc ctgtcccgga cgctccgatc
    32701 accgccacgc tggccgccgt caagaccgct cagggcgcat tcggcatcgg atgttctgct
    32761 gctgatgtgc cgccggcgtt ggtcagcggg aagatcgcgc tcatctcgcg cggcacctgc
    32821 accttcggga ccaaggtcgc caacgccaag gccgccggtg cggtcgcagt gatcatcgtc
    32881 aacaacgtcg ccggtgaccc gatcgccatg gttgccgaca ccgccttgtc ggcgcaggcg
    32941 caggcgctgc ccgcggtcat gaccgccctg tcggccaagg gcgctctgct ggccctggat
    33001 ggcgagttgg tgaccatcgg ttccgaccag gcctaccgga tcaccgacag caacgacatc
    33061 atggctgggt tctccagcca gggtccggtc gacgtgagct accgcgtcaa gccggacgtc
    33121 gtcgcccccg gcgtcaacgt cctgtcctcg atcccgatgt cctactgcga caacgacgtc
    33181 gagggctgct gggcgttctt ccagggcacg tcgatgtcca gcccgcacct ggccggaatg
    33241 gctgccgtcg tccgcgcggc gaaccccggt tgggatgcct ggcaggtccg ctcggcgatc
    33301 gccaacaccg ccaagctggg cgtgctgacg agcttcaccg atggcaccac ggtggacgtc
    33361 aacgtccaga aggtcggcaa cggcctggct gaccttgacg ccgccgtccg ggcgcaggtc
    33421 gctctgacgc ggcccagcgt gtccttcggc gcggtgccca acacggccgg ccagtcgttg
    33481 accgagacgg tgaccgtgac caacttgacc gatggggcgc tgaccctgcc gctgtcggtc
    33541 tcggagggcc cgggcgaagg tgtcttctcg gtctctcctc agtcggtgaa cctgccggcc
    33601 ggtggcagcg ccacggtgac ggtgacgttc acgtcggcca agggttcggc gctcggcaac
    33661 acccaggcga ccctgatggt cggctcggcc gcgcacgccg cgctgtacgc ctacctcaag
    33721 tagtcgctcg ctgcttacct cagtcgcccc cgcccggacc tggtccaggc gggggcgtct
    33781 gccgttgccc ccgacaccct cgacaccccc ccgggaccgg ccgggggggg tcggcagggg
    33841 tgtggtcagc gcgatgggtg agacccgact gtcacacctt cagagtcgac gcgagcggga
    33901 cgccgggtcg gtaggccagg tgcagatagc tcggcgcatc gagcaccgtg agatcggccg
    33961 gctcaccaac cgcgatccga ccccgcaacc cgagggtgcg cgcggagccg gcggtcgcgg
    34021 catacagcgc ctctgcgggg gtcatccgca actcccgcac ggcgagcgcg atgacgaacg
    34081 gcatcgatga ggtgtagcag gtgcccgggt tgcagtcggt cgccagcgcc acagcaacac
    34141 cggcgtcaag cagccggcgc gcgtcgggat aggggtgccg ggtcgagaac tcgacccccg
    34201 gcaacagcgt tgcgacagtg ccggtttcgg cgagtgcagc gacgtcggcg tcggacaggt
    34261 aggtgcagtg gtcgacgctc accgccccga gctcgcaggc caatcgcacg cccggcccgg
    34321 ggcccagttg gttgccatgc acgcgcacgg ccaggccggc ggcgcgaccg gcccgcagga
    34381 tcacccgggc ctcgtcctcg gtgaaggcgt gcgccgacag cggctcgcag aacacatcga
    34441 catacgtggc cgcaccctgg gcgacgaccg ccgccagcat cggcccggtc accaggtcga
    34501 ggtatgccgc gcggtcggcg gcatactcgg cggggacgac gtgcgccccg aggaaggtga
    34561 cgtgcggggt gacctcgcgg gccaggcgca gcagtcgcag ctcgtcctcg acgctcagcc
    34621 cgtaaccact cttgatctcg acggtcgtcg tgccctgacg ccgcaactcg gcgatccgcg
    34681 cggccaacag cgtgcgcaac tcgtcgtcgg tcgccccgcg cgtcgccgcc acagtcgtcg
    34741 cgatcccacc cccgtcgtat gccgtgcccg ccatccgggc cgcgaactcg gccgcccggt
    34801 caccggcaaa gacgaggtga gtgtgcgagt cgacgaagcc gggcatgacg gcgcacccgc
    34861 ccacgtcgac gcgcgaatct gcctgcgggg cagcgctggc cgggcctacc cagacaacct
    34921 ccccggcctc gacgaggacg gccgcgtcgg tgacgatccc caaccgagca acgtcgggcg
    34981 cctggcccgc agccgaccag ctcgaatcgt tggtgacgag ctcggcgatc cccgtgatga
    35041 gggttgcggt catgaagctg ctccgtcgtc gagggccgag atggcgtccg tcaagagtcg
    35101 gccgacgtcg cccagtcggt gttgtccggc cgagacgacc acttcaccgc cgacgacgac
    35161 cgtggtgacg tcggcgccgg tggcggcata gaggatctgc tcgggggcgg taccggcggt
    35221 gcgcggcgag cccagtcgca cggtgacgaa gtcggccagc gcccccgcgg ccagccggcc
    35281 cccgtcgggc cagccgaggg cggcataccc gttgcgggac aacgcatccg tgagctggcc
    35341 gggggtgaac cgcccgcgct caagggtgac gagccgctcg tgcgactcca gcccgcgagc
    35401 ctcctcgaac gggtcgacca ccgcgtgttg gtcggaaccg agggcgagcg gcacacccgc
    35461 gtcggccaag gcgcgagctg gcccgatgcc gtcggcgagg tcacgctcgg tcgtggggca
    35521 gaagcatgcg gtcacgccgg cgctggccag cgaggcgatg tcgtcgtcgg tgaggtgggt
    35581 cgcatggacc gccgtggtca tgggcccgag cgccccagcc ccctccagga gcgccgtcgg
    35641 cgtcacgccg tagaaggcca gcgcggtcgc gttctcggcg ggctgctcgg agaggtggac
    35701 gtgcaggggg cgccccactg acgccgccac gactcccgcc agcgcgtcct tcgggacggc
    35761 acgaaccgag tggatcgccg cccccatgcg tgccgtcggg gtgtcgctca tgagcccaac
    35821 ccgcgtcgac cacgcgtcga ccgagtcgtc ggaaaaccgt tgctgcacaa cgtcaagcgg
    35881 cagatggcca tcggggccga ggccgccggc gagatagatc gtgtcgagca gggtgagccg
    35941 gatgccggcc tcagcggccg cctggatgag cgccgccccc atggcgttcg ggtcggcgta
    36001 gggggtgccg tcgggttggt ggtgcacata gtggaactcc ccgaccgcgg tgaatcccgc
    36061 cagcgccatc tccgcgtatg ccgcccgcgc cagcgccagg taacgcgccg ggtccaggcg
    36121 gctcgcgacg gcatacatgc gctcgcgcca ggtccagaag gtgccgccgt ccccatgggt
    36181 ccgaccgcgc agggcacggt ggaacgcgtg ggagtgcgtg ttggcgagcc ccggcagggt
    36241 cacccctgcg agtcggacat cctcgggcct cgcctcgaca tccgccgaca cgccgacgat
    36301 ccgctcgccc atcatctcga ggcggacgct agcggccaat ccgcctggca accaggcgta
    36361 ttcgcaccag taggaggtca cgtggtcacc cagccaattc ggcagcagcg gcggccaagg
    36421 cgtggatacc ccggatgcag tcgtcccgct cggcgaactc ctggggcgag tgcgagacgc
    36481 cggtcgggtt gcgcacgaac agcatcgccg tggggatccc ggcattggcg aggatgcctg
    36541 cgtcgtggcc cgcgccggtg gcgagtaggg gagcctccgg caacaccgcg gccagacggt
    36601 cgcggagtct ggtgtcgaaa accgttgcgg gagtccagga cacctcgtcc acagtcgttc
    36661 cgtcttcgga cacctcatcg cgcagggtct ggacgagccc gcgcacggcc tccgggtcgg
    36721 cccctcgtgc gtcgagccag gtcgtgacgt ggtcggcgat cgcattgacg gcattgggcc
    36781 gggcgatgac cttgccgacc gtggccacgc acccacgttc gcgggccagc atccgcgccg
    36841 acgccacgac cgacgcgaag ccgagcatcg cgtcgttgcg gtccgccagc gccgtcgtcc
    36901 ccgcatggtt ggcctcgccg aagacatcga tctgccaccg gccgtggggc cagatctcgg
    36961 tgccgatcgc gaccgcccga gcggggtccc acagggccgg gtcgccgaag gggtcgtccg
    37021 ggtcgacgca cagagcccgg ccctgctcga catgcacctc gacgtaggcc gccacacggc
    37081 gcagggcgtc cgggtccggc cccagcgtgg ccgggtccct acccgcggcg gtccaggcct
    37141 gcgccatcgt caccccgtca ccatcggtca ggctgcgggc ccggtccggt gacaaggccc
    37201 cggtgaggat ccgcgacccg gcgcaggcga cgccgaaccg tcccccctcc tcgtcggcga
    37261 agttggcgat gccgagcggc atacgagggg tgaagccgtt gtcccgcaac gcatcgagcg
    37321 cggccagcgc gctgacgacg ccgagcgggc catcgaaggc gccgccgtcg gcgaccgagt
    37381 cgagatggga gccgagcacg atgccgggtc gcgcgtcggg gtccccccac caggcccact
    37441 ggttgccggc acggtcccgc acgacctcaa gcccgcgcgc cccgcactct ccggcgaacc
    37501 attcccgcaa ggtgtggtcc tcacgggtcc aggcgaaccg ctcgtaaccc cccgtcgcgg
    37561 ggttgcgacc gatgggcgcg aggtcggccc acatccggtc gaacgaccgg gcggctgcgc
    37621 cgtctggcgt gctgaagggg gccatggggt ctccttttcg ggctcagtcg cgcgcttgta
    37681 tgaggttggt gtcacttgta tgagtgtcta gcctcatcca agcgtcacta ccctcataca
    37741 agctgcgcac caccgaggtc ggcgcgggcg gcacactcac tgctcgcgca tggggatgcg
    37801 gacgccgagg tcggccgcga cgacgtccgc agcctcg
//
LOCUS       DAORPW010000007        36620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_143_length_36620_cov_715.687461, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000007 DAORPW010000000
VERSION     DAORPW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 36620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36620
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143_length_36620_cov_715.687461"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..3229
                     /locus_tag="PLB86_01400"
     CDS             80..3229
                     /locus_tag="PLB86_01400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRC63822.1"
                     /translation="MLTLRRAPALVVEAPTLDAQQQAALALDAPVARVLGGPGTGTST
                     VAVEVVLDRVDRLGLTPDQCLVLAPTRLAAAALRDRLTARLARTSTEPLARTHQAFGF
                     AVLRREAALRGAPAPRLLSGPEQDVVLRDLLAGHVASGGGPQWPERVAAALGTRGFRA
                     ELRDLLMRAVEHGVDAANLAELGRQHDRPEWVSAAAVLDEYDEVTALSQPGAYDPAWI
                     LTAAADLLEDDPEALERVREQIRLIVVDDAQELTWAAARLLRTIAHPGMQIILIGDPD
                     SAVQTFRGADPRLLWWDRWPALRDAPTVVLRTAYRTPTRLHDAASEVVRRIGATGGAD
                     RRGSQPVRDGGEVQVALLRSVAQEAGFIASTLRRAHLQEGMPWRDMAVLVRGRGRAGT
                     LRRVLSAAGVPLAPPDGEVPVRDEVAVRPLLALLDLCLRLARDPDAMIAPTEAVDLCL
                     SVLGGGDAVSLRRLRRALRRIELDGGGRRSSDELLAAALGDPVLSAQAGVDGEPLRRL
                     TRAILAGVGAARRTDDGAGWAPEVSPESVLWAIWSALGVAASWRRAALDGGSRGGRAD
                     RDLDAVVGLFDAAARYADRLPGSGPDAFLDHVLGEEVPGDTLALRAPAVDTVAVLTPA
                     AAAGREWRLVCVSGVQEGVWPDLRLRGSLLGSEHLVDVVTGRGGSVRAAQAAVRYDET
                     RLFHVAVTRASERLIVSAVRNDDEQPSVYLDLLDPLPADADGALGRPFTPFGRALTTA
                     GVVGALRQVAASVTDEVAAARAGRQLARLADDGVTGADPGQWWSLVPAADDRPRRGPD
                     QAVRVSPSKVEQFAKCELAWLLRASGGDGVKSASASIGTLVHDVVAELGDVDAPTLQA
                     EIERRWAQLGLTPGWVQERQRAEAHAMAGRAAAYFVSPAAAPWERVGVEVDALVEVGR
                     AVLKGRVDRLERHTDGSLRVIDYKTGSSKPTAAELARHPQLGVYQAAVEHGAFGELGT
                     RSAGAALLQLGKASNKSVTLQGQPALADDDDPGWASRLVEETAAGMGGGQFAATPGEH
                     CRMCPVVAACPARPEGQSI"
     gene            3229..>5816
                     /locus_tag="PLB86_01405"
     CDS             3229..>5816
                     /locus_tag="PLB86_01405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRC63823.1"
                     /translation="MATVRHSAAEVARAAGLAHAPTPEQTAVIEAPLRPMLVVAGAGS
                     GKTETMAARVVWLVANGLVAPDQVLGLTFTRKAAAELAERIDQRLRRLQAAGLWTPAP
                     DEEGAEVLGGTPTVATYHSYAGRLVREHALRLGYEPDSRLLSEAAAWQYAAEVVSRYD
                     GDMSEMGKAESTAIGAVVDLAGELAEHLVDTGTLREVLDRIVRAIDATPLAGTAKALP
                     ADLATLRGTLRARRALLPVVDAFGSLKRSRDAVDFADQVALAARLAVGFPDIGAGERA
                     RFRVVLLDEFQDTSEAQLVLLRSLFAAGDSGVPVTAVGDPNQSIYGWRGASATTLTRF
                     PAEFTDGSPTPVRQLATTWRNDASILKVANHLAAPLRASARVDVAPLAVRPDAGVGDV
                     VVARVGTHVEEAAYLADWLAGATSRAGTTAAVLCRKRSQFGPVMEALDAAGVPYEVVG
                     LGGLLLTPEILDLVSALRAIADPSRGDALMRLLTGPMCRLGAADLDGLHEWARFRQKV
                     VKAESQGRLAIDLDPGHRDEDDGVEGPGDALVDLAPEVLDEPSIIEALDDLPPEVWRG
                     TEGQYVGAGALVRLKGLSTALRRLRRLGALPLPDLVGEAERALGLDVEVLSRPGYTPD
                     TARAHLDAFADVAADFASSADRPTLIGFLAWLDAAVAQERGLERATLEPSAEAVQILT
                     IHAAKGLEWDVVAVPGLVEGTFPAREGSPTPKHNGARWVVPDPTPGGWLVGLAGVPFA
                     LRGDRDGLPTLQVEGASDSKALSERVAAFRVEEGLRDLDEERRLAYVAVTRARRRLLL
                     TASVWTNTQRHKVTSRFLREVVRARDELGVRVARWDAMPDPAAVGTNPVADETVRVLW
                     PTPPDQ"
     assembly_gap    5817..5916
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5917..6652
                     /locus_tag="PLB86_01410"
     CDS             <5917..6652
                     /locus_tag="PLB86_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRC63824.1"
                     /translation="TGEGVAAYPRAALLAPRGGGADDPYAADVDLLLAERALARETGP
                     QVVEVPRHLSASDIVRLAEDPERFAGERRRPMPAEPALEARRGTAFHAWVEQHYARAT
                     LLDSAELPGAADDDPGSDAELPQLKETFLASEWAWRTPIDVEVAIETVIDGIAVRGRI
                     DAVFGRADGGVTVVDWKTGPQPSGADAAHRALQVGAYALAYARLRGLLPDQVDAAFYY
                     ARTGTTVRPTLPDEAELIRLLGSIAD"
     gene            complement(6649..7989)
                     /locus_tag="PLB86_01415"
     CDS             complement(6649..7989)
                     /locus_tag="PLB86_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRC63825.1"
                     /translation="MSPESRSPAFLAALASAAVPGLDPVSARAVAPGPAGEFDAAIVI
                     DEQDRPWFVRCPRTPAAGARLESAAALAALLARRLPFQVPVPKGFAALPDGRAGVYPV
                     LPGRPLAFDALPAGPGLAADLGRAIAAIHNVDRGVFDEAGMPSYDADSYRTRHLADLD
                     RAAATGHVPTALLSRWEGALDDVTLWRFAPAPVHGGLTGSQVLAGFDSDEDAATGRVR
                     AVVGWEDAKVADPADDFAAIVTLAGPAAVESVLEAYANSRIERPDPHLRRRARLAAEF
                     AQLTSLLTAVTLGDPDRVRAMARRLVELERRVTDADLKAPEPVSVVPREVIVPKTVAA
                     ASPAAANGSGSGEDTVAVDPDTLFDDTAPLHGTAAHDDSVTHDDSVTLDGTVTLDDAA
                     ALDDTVAMDPRTLFDATEEIPTAHGPASPSRPDAPATPDAPKQQSVPATPDEPA"
     gene            8053..9072
                     /gene="nudC"
                     /locus_tag="PLB86_01420"
     CDS             8053..9072
                     /gene="nudC"
                     /locus_tag="PLB86_01420"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRC63826.1"
                     /translation="MEQTPGLRGLLLSHGGPDRAAHLREDAGLLDRLLADAGTRVVAV
                     RGDRVLVAPVPAHSMAPTADPSPAPADGAAGALGLVLRSPSAADGGLLAVFLGLHPGE
                     DGERTAYLGVVEAPTDGDGDAGWQTLRAAGALLPDLDASLAATLLALANWHRSHERCS
                     RCGAPSEPTSAGWVRRCTRDGSQHFPRTDPSVIMSVIDDDGRLLLGRGVTWPANQFSV
                     LAGFVEPGESLEAAVAREVLEESGVVVTEVSYLGSQPWPFPSSLMLGFTARAVTTALT
                     HDPTEMAEVRWVTRAEYAALLRSGAIRVPGSISIARRIIERWLGADLEVAAGHAVIEG
                     WRPTT"
     gene            complement(9069..9326)
                     /locus_tag="PLB86_01425"
     CDS             complement(9069..9326)
                     /locus_tag="PLB86_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097573 - glutathione oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin"
                     /protein_id="HRC63827.1"
                     /translation="MSEFTPAPGTVTMFTTTWCGYCRRLKSQMEREGIAFTEVDIEVQ
                     PEAADVVMSVNGGNQTVPTLIFPDGSAATNPSIGQVKARLA"
     gene            9517..10326
                     /gene="ppk2"
                     /locus_tag="PLB86_01430"
                     /pseudo
     CDS             9517..10326
                     /gene="ppk2"
                     /locus_tag="PLB86_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006213342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
     gene            complement(10640..11209)
                     /locus_tag="PLB86_01435"
     CDS             complement(10640..11209)
                     /locus_tag="PLB86_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63828.1"
                     /translation="MILPKVRDPRLVTIRRGGLLTDPDHHLLALWAAACAEHVLDLFE
                     AECPSDLRPRHAIAGARAWAAGELAMMQSRAAGGHAMGAARPLSGAARFAAYAAGQAA
                     CIPHVPEHDLGAAAYAIKAAQAAAAAGDDGEAAARRECQWQLDQLPDPIRALVLDDQA
                     RRNPICWSVFTEPGPLAAGPTHPSGGLQR"
     gene            11399..12730
                     /locus_tag="PLB86_01440"
     CDS             11399..12730
                     /locus_tag="PLB86_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HRC63829.1"
                     /translation="MSSTSATLSGGASVADRRAAFVAARSALDGLTGLLCQVAGAELG
                     HLVGELDALASRAAGAVVAVVAEADSRGEISASQAGTSTQWVARHAPSLAAEGAGQVA
                     RLVQECRLPELATVRAAVEGGTVSVGVGLGVVSEFQALRPMLRNEARPTVLDGLVQMG
                     AEQGRRGVRRLRPALLAQFGDPGVFQEVQDAAGRLVALDRAVGDVLGAFTYRLVVDAQ
                     GKAALEAAIGPLSAPVPGPDGARDPRPPERRRGEALIEVCRRATAAGGIPPSGVKSTL
                     MVTMPFADLSNRLGAGEVLGSSEAGELIAPETVRKLACDAGVIPVVLGGEGEVLDLGR
                     ATRLFTAGQTAALWLRDRVCSFPECSIPAHWCDAHHLIHWLDGGASQLSNAALLCGRH
                     HTVVHRDRLFGQVTSDGVDWDRRLGSYDRALAAPGHGGKAGEAAGPDPPAA"
     gene            complement(12826..13584)
                     /gene="fabG"
                     /locus_tag="PLB86_01445"
     CDS             complement(12826..13584)
                     /gene="fabG"
                     /locus_tag="PLB86_01445"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRC63830.1"
                     /translation="MSTTPRTAIVTGAARGIGAATAKRLAADGHAVAVLDLDEAACAA
                     TVSEIVAAGGRALAVGVNVADSASVAAGVERVAAELGGPTILVNNAGITRDNLLFKMT
                     EEDWDSVLSVHLRGAFLMSKACQKYMTEATFGRIVNLSSTSAQGNRGQANYAAAKAGM
                     QGFTKTLAIELGKFGVTCNAIAPGFIQTEMTKATADRLGVSFEDFIAFSAKEIPVARV
                     GQPEDIAATVSFLTSDEAGFVNGQVIYVAGGPKD"
     gene            complement(13700..14722)
                     /locus_tag="PLB86_01450"
     CDS             complement(13700..14722)
                     /locus_tag="PLB86_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRC63831.1"
                     /translation="MTDTPAAYAVSDLLDILDLREVGTVHTGWAVASPSESPTAAVTA
                     DIATATATATAAVADLAVEVGAGSRVARVFAGPSQKQRYQRTFGGQLLAQAIIAAGRT
                     VAADPAGAGRPLHSTHTTFLVSGADTDEVHYVVEPLSDGRSFSTRRVSALQHGTLLAV
                     VTASFQEASPGLDHAEPMPPAPSAESLPNVAEALAGVPEPYAVVCILDGPIELRHVEG
                     HLYAGARPEQVARQGVWLRSRRSMPDDPIIHAAFLAYASDYSILESVLRRHGTAWSDP
                     RLRQASLDHAMWFHRPGRADEWVLHAGHSPSASGGRGLGLGRMYAADGTLLASIAQEG
                     MLRLKG"
     gene            complement(14785..15735)
                     /locus_tag="PLB86_01455"
     CDS             complement(14785..15735)
                     /locus_tag="PLB86_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HRC63832.1"
                     /translation="MRLPDSAADSVADLLDVLDLTCLGEARIVVEGAPGDEETDLGES
                     TPIVFVGRSQKQAHGRVFGGQVLAQSLVAAARTVEPVDGVARPIHSLHAYFVRGGDDN
                     HPIRFVVERVRDGASFSTRRVQAVQYGQTILSMGCSFQEPADGMDHQDPMPPAPDPDD
                     IPTLAEALGEHLDPSAAQFFAARRPIELRHVEGNIYVKPGRQRAARQSVWLKARAELP
                     DDPVIHAAVLAYASDYPMLEPVLRRHGVAWQDPRLRPASLDHAMWFHRAPKADDWVLY
                     TMSSPSASGGRGLGVGRMFSADGALMASVAQEGMLRLKEG"
     gene            15776..16315
                     /locus_tag="PLB86_01460"
     CDS             15776..16315
                     /locus_tag="PLB86_01460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRC63833.1"
                     /translation="MPDPILASTLASTLASTLASAPAATPAAATVAPDPTEVLVPLRW
                     SDMDAYGHVNNTQFLRLLEDARVAALPRWFPEGLALLERGILVVRHEIEYRAQLDYRP
                     EPVAIDLWVTRIGGAGYDLAYVVRDPHGVGSGTVYAVAETSLALFDFAANTPRRATAV
                     ERECLTAHLGAPAPLRKRR"
     gene            16323..17060
                     /locus_tag="PLB86_01465"
     CDS             16323..17060
                     /locus_tag="PLB86_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63834.1"
                     /translation="MTDLVFPDQQGYADLATLVRRARQADPDAAVRLQVTGAVLRVWV
                     GVLPGTGLLADGAAVGVRGIELANEDAGGSTSEAGALDLVCASAALSDRFAYDGVGAS
                     RLSVPPASTYAAWAGALPAVAGWSSIGAISAQALIDAARAGIAEIAEGVDARQTGAAA
                     GSRAVQELRRRVWARAVPIPAGVDDGPVNGADDQRPTGFEGVPAGAAFAAYVLGFLPE
                     PSATVRVWRSGRWVRLQTAVGHVVARP"
     gene            complement(17022..>17991)
                     /locus_tag="PLB86_01470"
     CDS             complement(17022..>17991)
                     /locus_tag="PLB86_01470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63835.1"
                     /translation="ASASASDARRLADAAVAMGHVGALDDGALDATADAPVADPAVRE
                     AADPADDPADERRQHRRTARLGAVVAGAAGVVQLAIHERPPAVSFLTAAAVLALIAVA
                     IAAPKLLPPGTFRSARGLPSVVLTRALLNGSFLGAFAYLPLMLTQEKGLDATSAGLVL
                     AAGSLGWSAGSWAQGRSRRSGIDERTGLVVLGASLLAVGWAAFVGLTLGHAPVWTFAA
                     AVAVGGLGMGLGSTTLSGLVLELSPDHEHGRASAALQLADVLGTVVGIAAATAAFGIW
                     HTTGAHRGFVIVWIGLAALGALGIVSAARCASETSRSGDDVPHRSL"
     assembly_gap    17992..18091
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18111..18899)
                     /locus_tag="PLB86_01475"
     CDS             complement(18111..18899)
                     /locus_tag="PLB86_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRC63836.1"
                     /translation="MSAADYPYFAPPPFGLAHRGGASHPANVGIENTLAAFATAVDLG
                     YRFLETDVHATADGRLVAFHDEHLDRVTDRAGAIAELPWSEVAAARIAGSQPIPSLDD
                     LLDAFPEARINIDIKAPGAVVPLWETIRRHAAYDRVCVGSFGERRLHAFRRLAGARVA
                     TAAGQLGTAALRYAPGRLSTLAHSPGQVLQIPTTHEVRGRTFPLVTPGLIAAAHRFGL
                     QVHVWTIDDPDEMVRLFDLGVDAIVSDRIDVLRDVLAARGAWPT"
     gene            complement(18926..19942)
                     /locus_tag="PLB86_01480"
     CDS             complement(18926..19942)
                     /locus_tag="PLB86_01480"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRC63837.1"
                     /translation="MSAATTTSERVAVFGTGSWGTAFASVLAEAGCEVRMWGKFEDEV
                     ADIQRHHASVRYLPDLRLPDSLTATTDPAEALAGAGIGVLAVPAQTLRANLAEWGPLL
                     PPGIVLVSLMKGLEKGTSKRMTEVVGECLDLAPEQIVAVSGPNIAREIAAREPAATTV
                     ASASLATAHRVADACATPAFRPYVHDDVVGAELGGSVKNVIAVASGMTEGLGLGHSVT
                     AAVITRGLTEMRLLGEAMGAHPATFAGLAGVGDLVVTCLSPHSRNSTFGRNLGTGMTV
                     AEVVAVTKQTAEGVSSCGAILALARSHGVDMPIVANVTAVVQEGRTAPEFAQVLMDDM
                     FGGR"
     gene            20114..21742
                     /gene="pruA"
                     /locus_tag="PLB86_01485"
     CDS             20114..21742
                     /gene="pruA"
                     /locus_tag="PLB86_01485"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HRC63838.1"
                     /translation="MDALFRVPAPANEPVLGYAPGSSERATLEGELARIGGEQIDLPS
                     TIGGRKVMGGGRTGKVVQPHAHRKVLGTMKLVTPMEAQSAIDAAKAAAPMWRDMPFAD
                     RAAILLKAAELLSGPWRATLNAATMLGQSKTAPQAEIDSACELIDFWRINVHFAQQIL
                     AEQPPLNSKGVWNRSDYRSLEGFVYAITPFNFTAIGGNLPTAPALMGNTVVWKPARTQ
                     QLAAHYTMLILEAAGLPPGVINLVTSDGATLSNAVLADRDFAGIHFTGSTGVFHSMWE
                     QVGANIGRYKTYPRLVGETGGKDFVVAHPSADIDVLRTALVRGAFEFQGQKCSAASRA
                     YVPASIWKRLSKDLADEVNSLTQGDVTDFGNFMGAVIDGGAFADHKAAIDRAHATPGV
                     SVVAGGTYDDSEGFFVRPTVLKCDDPTDESLRTEYFGPILSVHVYPDRSYDRMLDQME
                     SFAPYALTGSIIAQDRAAIVAAAHKLRYAAGNFYINDKPTGAVVGQQPFGGGRASGTN
                     DKAGAAQNLMRWTSVRSIKETFVPPTDYRYPSMG"
     gene            complement(21845..22357)
                     /locus_tag="PLB86_01490"
     CDS             complement(21845..22357)
                     /locus_tag="PLB86_01490"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HRC63839.1"
                     /translation="MQITETLVYPAAIEVVFDMMTDEAFHSRVCEATHAMSYSATVSR
                     QSDGATVITHREMPTNSFPDFARSMVGQSIDIVETIVYGPPGPHGTRIGEMSISMGSA
                     PIHLKGDIRIIPADDGTEVIIEGNLKASIPFVGGKVESAAAPSVISGIHKEYGVGLAW
                     LAERGHTDQD"
     gene            complement(22369..22821)
                     /locus_tag="PLB86_01495"
     CDS             complement(22369..22821)
                     /locus_tag="PLB86_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63840.1"
                     /translation="MVTTLRGTDPGSVSRLGGLLRTTADTLSGDLAALPATSPLAQLA
                     TATADQVDAIGSALQVHAQELAEAAAAYSRLQERAAALGLIVTDWTVTEPFGLIGSDV
                     AARRGVERPQVQAQADRLASRLARGRAQLARQLAQASQQVARASAAAR"
     gene            complement(22815..23174)
                     /locus_tag="PLB86_01500"
     CDS             complement(22815..23174)
                     /locus_tag="PLB86_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63841.1"
                     /translation="MPDGERPAHRSTRRDHGAPADVLAQVRQHALDASESLFAVDPML
                     GDPLVQRGVDEFVEQVVDALRVIAQLSQQRDPSWAGAEEHHAGSDVQHRVTPDVARPG
                     GRDATRRAGSDMDRPRW"
     gene            23298..28205
                     /locus_tag="PLB86_01505"
     CDS             23298..28205
                     /locus_tag="PLB86_01505"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HRC63842.1"
                     /translation="MTTRNGLFTMTISLEQSRGNVLRSAAGLAQKGSGDPVEPLLTRY
                     YRHVSTEDLLARSPEDLLGAALSHLDLAHDRPVGTASVLVDNPTVESQGWSSGHTAIQ
                     IVIDDMPFLVDSVTMALTRRGLGVHLLVHPQLIVRRDARGVLEEVLDVDKAPSGEFGV
                     GVESWMHIEVDRMSNAADRDSLSAELQSLLGNVRDAVEDWPKMRERCVEISDGLRAAA
                     PAGIDGPEVAQGVRFLDWLAANHFTFLGYRQYVLGDDGGETVLHPVTGTGLGLLRYDA
                     AGSTAFARLSPPAQARARDRELLIITKANSRATVHRPVYLDYVGVKTFDAAGDVTGEH
                     RFLGLFAAAAYTASIMDIPIVGDRAREVLDRVGYSGDSHSGKDLLQVLESYPRDELFQ
                     TDADMLAEIAETVVHLHERIRTRLFLRKDVYGRFMSALVYLPRDRYNTAVRLRMEALL
                     RKAFAADSVDYTTRVSESPLAQVHFVLRVPKGAQVKDVDAAALESEIVDATRTWDEDL
                     LESARSEHGEELGARFAALYGKAFPEAYKEDFTPRVAIADVRRIEVLGSDEAVEFNMY
                     AEAGGPRDERRFKLYTRSPLSLTRVLPVFTHMGVEVTDERPYVLHRADGARVHVYDFG
                     LRATSAALWGEGAEREHCRARFQEAFSAIWRGRAESDGFNALVLQAGLAWREVLVLRA
                     LAKYLRQTQSTFSQDYVESALSANAGIARDLVELFAIRMDPTRYAGLPGAERTDAAQA
                     VVERITDSLVQVQSLDHDRIVRALLAIVLAATRTNHYQVDAEGEPKSYLSVKLDPKAL
                     PDLPAPRPMFEIWVYSPRVEGVHLRFGKVARGGLRWSDRREDFRTEVLGLVKAQMVKN
                     AVIVPTGSKGCFFPKQLPEPAVDRDAWLAEGIAAYQIFISGLLDVTDNRVGGEIVPPA
                     NVVRHDADDSYLVVAADKGTASFSDIANAKAQDYGFWLDDAFASGGSAGYDHKGMGIT
                     ARGAWESVKRHFREMGHDTQTQDFTCVGIGDMSGDVFGNGMLLSEHIRLVAAFDHRHV
                     FIDPTPDAASSFLERQRLFALPRSSWADYDRSLISAGGGVFERSAKAVPVSPEMAAAL
                     SIDPTITALTPAELMKAILLAKVDLLWNGGIGTYVKSIGETNAEVGDRANDSIRVNGR
                     ALRCRVVGEGGNLGCTQLGRIEAAQAGVRINTDAIDNSAGVDTSDHEVNIKILLGQLV
                     RDGDMTIKQRNTLLASMTDEVAAQVLRDNYEQNVLLGNARAQTLVMLPVHQRFIHYLE
                     QNGDLDRGLEYLPGDAEIVGRQADGRGLTSPEFSVLVAYSKLDLKAALLPTALADDPW
                     FAGTLAEYFPTRLRNEHANQLDQHPLKREIIINSVVNSLINRGGITFAFRAAEETGAT
                     PEQVARAFIVCREVFDLAGFSAAVESLDATLPAKVQTVLYLEFRRLLDRALRWFLANR
                     PGTIDVTEEIARFLAPVQEHAALVPEVLQGSERRRLDRRAAELAALGVPEALAMRTAS
                     LLDVFSMLDIVEIAQATGTPSQDVLRVYYHASERFGIDDMLFRVSQLARDDRWDSAAR
                     GALRDDLYAVLDGITRNVLATSDPKADAKSRFNQWAKANQEALDRARSGLQAILRLES
                     ANIAALSVALRTLRAFVKTATPTDG"
     gene            28232..29698
                     /locus_tag="PLB86_01510"
     CDS             28232..29698
                     /locus_tag="PLB86_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HRC63843.1"
                     /translation="MATLSELIQEHAALPQVDQEWLRLLVSDWQLLSDLSFADLVLWV
                     RSSPDRWLAVAHIRPTTGQTVYFRDQVGRVAGTSRVELLEAAYLQRRIVRERDSEWDP
                     DDPVREESIPVVRAGGAIAVLTRHTNLSMLRTPSRLELTYLATADALSRMVANGEFPH
                     PTAPTGLRRGAPRVGDGVIRLDTEGVITYASPNSVSALHRLGYVGEILGTSLAELVTS
                     VLHDTSPVDEGLPLVLTGRQPWRTEVTSAGQVISMRAIPLSESGQRIGAIVLARDISE
                     LRRREQELMTKDATIREIHHRVKNNLQSVAALLRLQARRMPDAQGRAALEEAVRRVGT
                     IALVHDTLSHGLDETVEFDQVADVTIRAVVEVAAGGRSVAVRRTGSFGRVRAEDATSL
                     AMVVSELVQNAAEHGVASQGGTINLEVKRKEGKDETDVLTVTVTDDGVGLPGGRLPAG
                     VGLGTQIVQSLVQDLRGKISWGPAEPQGTRVRFTARLR"
     gene            complement(29801..30049)
                     /locus_tag="PLB86_01515"
     CDS             complement(29801..30049)
                     /locus_tag="PLB86_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRC63844.1"
                     /translation="MDWRDRAACLDEDPELFFPIGNTGPAILQIEEAKAVCRRCEVLD
                     TCLRWALESGQDAGVWGGLSEDERRALKRRNARARRAG"
     gene            30413..30799
                     /locus_tag="PLB86_01520"
     CDS             30413..30799
                     /locus_tag="PLB86_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63845.1"
                     /translation="MSTGGFRPRVSSARRVAAIICAIEALAMAGFGIYGLVELARGGG
                     TGTARVITESVLILAFAAGMALLARLWLGRSGWPGTPTVVWHALLIPVVVSMAQAGQW
                     LVTLSLSVAIVLSIGAATVARGSGSD"
     gene            complement(30813..31574)
                     /locus_tag="PLB86_01525"
     CDS             complement(30813..31574)
                     /locus_tag="PLB86_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRC63846.1"
                     /translation="MSPVFAHGQLRLYLLVLLAEAPRHGYEVIQDLETRFGGLYTPSA
                     GTVYPRLAKLEEEGLVERTDEGRKATYRITEAGLAEVRARHREVEDLQADLDQSVRRL
                     AEQVRATVQGRSADLRAELKAAAREARTTARPAGSTSGAGWPQGSSPGWGQSPTDRTA
                     GAGAYGGTSDRSGPRSGSRSGSGSGGGSGGGSGAGTFAGADWTDVERAASEVREQARR
                     AWRQHGLTAEQTAEIVEILADTSRRIAQVLRMPRD"
     gene            complement(31571..>31776)
                     /locus_tag="PLB86_01530"
     CDS             complement(31571..>31776)
                     /locus_tag="PLB86_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63847.1"
                     /translation="SLTLRANSVSGDVILLRAAAPAAGPSGSGDAGPQDRSPEVSPGG
                     PQDAPARDEVRESSIDDEGQDTP"
     assembly_gap    31777..31876
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31877..32730)
                     /locus_tag="PLB86_01535"
     CDS             complement(<31877..32730)
                     /locus_tag="PLB86_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HRC63848.1"
                     /translation="MAEHWLVDGPRVIDVGSDRERVEKVVVGIIGGRVNVVTHDDGYG
                     ARVEVSNVEGLPLRVSWDGRTLKVMHTKDGGSTVLDTLRRLVEQVSGQHADVSISVPA
                     SARATVSTVTAPIVVSGLRRGLAANTVSGEMTISDVQGRSTINTVSGATECADLVGDA
                     KINSVSGSVTVQSSDIPRARINTVSGEVALDLVNTHLDVQSNSVTGDVTIRAPFSGYA
                     VTATSATGQVIVDGQSLHGASSGSMPPGGWGDCAPGGSSGRGTLRSGDESLTLRANSV
                     SGDVILLRA"
     gene            complement(32836..33522)
                     /locus_tag="PLB86_01540"
     CDS             complement(32836..33522)
                     /locus_tag="PLB86_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRC63849.1"
                     /translation="MTGHDGPAYPSATDGEAAPEFTPSASFTVDDDGRRDIGLCFVGD
                     SFTVGFGDPKALGWVGRVVSRTHHPDVEITAYNLGVRGQDSGDVMARWRAECMPRWVG
                     RGERRLVVAVGANDLGQGLTTARSRLNLANILDDASAKGIATFVVGLTPSLDPELNAR
                     IGVLAQAQADVCSRRGVTYVDCYRPLVAHEQWAADLAAGDGVHPGQAGYGLIAWLVLH
                     AGWNAWMQLG"
     gene            complement(33545..33721)
                     /locus_tag="PLB86_01545"
     CDS             complement(33545..33721)
                     /locus_tag="PLB86_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HRC63850.1"
                     /translation="MYFTDRGIEELDARRGDEEVTFSWLAEHLRTFVDLHPEFETPVE
                     RLATYLARLDDDEE"
     gene            complement(33818..34936)
                     /locus_tag="PLB86_01550"
     CDS             complement(33818..34936)
                     /locus_tag="PLB86_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC63851.1"
                     /translation="MTDDLDELSSLARTFFAREVTPNAERFAEQHQVDRETWLKAGAA
                     GLLGLSVPEEFGGGGGTFAHEAVVFQEQGWAGDDALGLHIHSGIVPHYIVEFGTPEQK
                     RRWLPKLASGEWVGAIAMTEPGTGSDLQAVRTTARRDGDDYVVNGSKTFITNATHADL
                     VIIVCRTGAPGAKGISLLVAEVNDLPGFTRGRVLAKIGQHGQDTRELAFSDMRVPAAN
                     LLGEEGHGFGQLMEQLPQERLVVAVGAVAAAEYAVRLATAYSKEREAFGRKILDFQHT
                     RFVLSEVSTDALAARTFLDHCITEHLAGRLDAVLASRIKYWATDVQCSVIDRCLQVFG
                     GYGYMMEYPIARLYAAARVQKIYAGTNEIMKELISRSL"
     gene            complement(35148..>36620)
                     /gene="kgd"
                     /locus_tag="PLB86_01555"
     CDS             complement(35148..>36620)
                     /gene="kgd"
                     /locus_tag="PLB86_01555"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775736.1"
                     /note="kgd; produces succinic semialdehyde; part of
                     alternative pathway from alpha-ketoglutarate to succinate;
                     essential for normal growth; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HRC63852.1"
                     /translation="DDPSMTQPLMYNLIEAKRSVRKLYTESLIGRGDISVEDASAALR
                     DYQQQLERVFLETREAQAAPAASQTAYVDSTSDVAGHGGLEKPTAQALDDVSRPRDWR
                     QTAISVDTLNTLGDAWVRPPDGFTVHPKLKALMDRRAAMVREGGIDWGMGELLALGSL
                     LMEGTPVRLAGQDSQRGTFVQRHAVLIDKITGREWTPLHHLQEGQAKFWVYNSLLSEY
                     AAMGFEYGYSVERPDALVLWEAQFGDFANGAQTIVDEFISSSEQKWAQRSSVVLLLPH
                     GYEGQGPDHSSARIERFLQLFAEDNMTIAYPSTPASHFHLLRRQAYARPRRPLIVFAP
                     KSMLRLKAAASMPQDFTTGGFRPVLPDTRHLDGGAVTRVLMASGKVVWELEAERDKLG
                     DTSTALLRIEQLAPAPAEELAAALRGYPNAELVWVQDEPKNQGAWPFMALNLPELLAE
                     QGENRPLRVIARKASASPASGSHKKHELEQAALLGAAFAR"
BASE COUNT         5312 a        12533 c        12900 g         5575 t
ORIGIN      
        1 cccgcaaaga tgaccgtgcg agcgctatct ccgggggagg cagcggaggg gtgtcggtcg
       61 cccgtgatgg gatgagtgca tgctcaccct gcggcgtgca ccagcgctcg tcgtcgaggc
      121 acccaccctc gacgcgcagc agcaggctgc cctcgcgctg gacgccccgg tcgcacgggt
      181 gctcggcggg cctggcacgg gcacctcgac cgtggccgtc gaggtggtcc tcgatcgggt
      241 cgaccggctg gggctcaccc ccgaccagtg cctggtcctc gctccgaccc ggctcgccgc
      301 cgctgctctg cgcgaccggc tgaccgcgag gctggcgcgc acgtcgaccg agccgctcgc
      361 gcgcacgcat caggcgttcg ggttcgcggt gctgcgccgc gaggcagccc ttcgcggtgc
      421 gccggctccg cggctgctca gcggcccgga gcaggacgtc gtcctgcgcg atctcctcgc
      481 gggtcacgtg gcgagcggcg gcgggccgca atggccggag cgggtggccg ctgccttggg
      541 cacgcggggc ttccgagccg agttgcgtga tctactcatg agggccgtcg agcacggagt
      601 cgacgcggcc aacctcgccg agctcggacg ccagcacgac cgccctgagt gggtgtcggc
      661 agcagcggtc ctcgacgaat acgacgaggt gacggcgctg tcgcagccgg gtgcctacga
      721 ccctgcctgg atcctcaccg ccgctgctga cctgctcgag gacgaccccg aggcgctcga
      781 gcgggtgcgc gagcagatcc ggctgatcgt cgtcgacgac gcgcaagagc tcacttgggc
      841 cgcagctcga ctccttcgaa ccatcgccca tcccggaatg cagatcatcc tcatcggcga
      901 ccccgacagc gcggtccaga ccttccgcgg ggccgaccct cggctgttgt ggtgggaccg
      961 gtggcccgct ctgcgggacg cgcccacggt ggtgctgcgc acggcatacc ggacaccgac
     1021 ccggctccac gacgcggcga gcgaggttgt gcgccggatc ggggcgaccg gcggcgcgga
     1081 ccggcgcggg tcccagcccg ttcgcgacgg cggtgaggtg caggtggcgc tgcttcgctc
     1141 ggtcgcccag gaggcggggt tcatcgcctc gacgctgcgt cgcgcgcacc tgcaggaggg
     1201 tatgccgtgg cgcgacatgg ccgtcctcgt ccgaggccgt ggtcgcgcgg ggactctgcg
     1261 ccgggtgctt tcggccgccg gggtgcctct ggccccgccc gatggtgagg tcccggtgcg
     1321 cgacgaggtc gccgtccggc cgctgctcgc gctgctcgac ctgtgtctgc gcctggcccg
     1381 cgacccggac gcgatgatcg cgcccacgga ggccgtcgac ctctgcttgt cggtgttggg
     1441 tgggggcgac gcggtgtcgt tgcgccggtt gcgccgggcg ctgcgccgga tcgagctcga
     1501 cggcggggga cgacggtcga gcgacgagtt gctggcggca gccctcggcg acccggtgct
     1561 cagtgcccag gccggtgtgg acggggagcc cctgcggcgg ttgactcggg cgatcctcgc
     1621 cggtgtcggg gctgcccggc ggaccgatga cggggctggc tgggcccccg aggtcagccc
     1681 cgaatcggtg ttgtgggcga tctggtctgc ccttggcgtc gcggcgagtt ggcggcgcgc
     1741 ggcgctcgac ggcgggagcc gcggaggccg ggccgaccgc gacctcgacg cggtggttgg
     1801 tctgttcgac gcggctgccc ggtatgccga ccggctgccg ggctccgggc cggatgcctt
     1861 ccttgaccat gtgctcggcg aagaggtgcc tggcgacacc ctcgcgctgc gcgctcctgc
     1921 ggtcgacacg gttgcggtgc tcacccccgc tgctgctgcc gggagggagt ggcgattggt
     1981 gtgtgtgtct ggagtccaag agggcgtctg gccagacctg cggttgcggg ggtcgctgct
     2041 cggctcagag cacctcgtcg acgtcgtcac cggtcgaggt ggctcggtgc gggcggcgca
     2101 ggccgcggtg cgatacgacg agacccggct gttccacgtc gccgtgacgc gggcgagcga
     2161 gcggctcatc gtcagcgccg ttcgtaacga cgacgaacag ccgtcggtct atctagacct
     2221 gctcgacccg ttgccggccg acgcagacgg cgccctcggg cgacccttca caccgttcgg
     2281 tcgcgcgctg accaccgccg gagtcgtcgg tgccctgcgt caggtggcgg cgtcggtcac
     2341 cgacgaggta gcggccgccc gggccgggcg gcagttggcg cggctcgctg acgatggcgt
     2401 gaccggagcc gacccggggc agtggtggtc cctcgtcccc gccgccgacg atcgtccccg
     2461 gcgtggccct gaccaggccg tgcgggtctc tccgtccaag gtcgagcagt tcgccaagtg
     2521 cgagttggct tggctgttgc gggccagtgg cggcgacggc gtcaagagtg cctcggcatc
     2581 gatcggcacc ctcgtgcacg acgtcgtcgc cgagctcggc gacgttgatg ccccgaccct
     2641 gcaggccgag atcgagcgcc gctgggcaca actcggcctc acccccgggt gggtgcagga
     2701 gcgccagcgc gccgaggctc atgccatggc cgggcgcgcc gcggcctact tcgtctcgcc
     2761 ggcagccgcc ccctgggagc gggtcggggt cgaggtcgac gcgctggtcg aggtggggcg
     2821 tgcggtgctc aaggggcgcg tggaccggct cgaacggcat accgacgggt cgttgcgggt
     2881 gattgattac aagaccgggt cgagcaagcc gaccgcggcc gagttggctc gccatcccca
     2941 actcggggtc tatcaggcag cggtcgagca cggcgccttc ggtgagctgg ggacccgatc
     3001 ggccggggct gcgttactgc agttgggcaa ggcgtcgaac aaatcggtca ccctgcaggg
     3061 gcagcccgcg ctcgctgacg acgatgaccc cggctgggcc agccgcctcg ttgaggagac
     3121 tgcagcgggc atgggcgggg gacagttcgc ggcgacgccg ggcgagcact gccggatgtg
     3181 tccggtcgtg gcggcctgcc cggcccggcc cgaagggcag agcatctgat ggcgacggtt
     3241 cggcacagtg cagcagaggt cgcccgcgcg gcggggttgg cccacgcccc gacgccggag
     3301 cagacggcgg tcatcgaggc accgctgcgg ccgatgctcg tggtcgccgg ggcaggtagc
     3361 gggaagaccg agacgatggc cgcacgagtc gtctggctcg tggccaacgg gctggttgct
     3421 cccgatcagg tgctcgggct cacgttcacc cgcaaggcgg ccgccgagct cgccgagcgg
     3481 atcgaccaac ggctgcgccg cctgcaggct gcggggctct ggacgcccgc gcccgacgag
     3541 gagggtgccg aggtcctcgg cgggacgccg accgtcgcga cctaccactc gtatgccggt
     3601 cggctggtcc gcgagcacgc cctgcggctg ggctacgagc ccgactcgcg actgctgtcc
     3661 gaagccgccg cctggcagta tgccgccgag gtggtctccc gctacgacgg ggacatgtcc
     3721 gagatgggca aggcggaatc gaccgccatc ggggcggtcg tcgacctcgc gggtgagctg
     3781 gcagagcatc tcgtcgacac cggcacgctg cgtgaggtcc tcgaccggat cgttcgcgcg
     3841 atcgacgcga ccccgctggc cggcacggcg aaggcgctgc ccgctgacct cgcgacgttg
     3901 cggggcacgc tgcgggcccg ccgggccctg ttgccggtcg tcgacgcctt cggctccctg
     3961 aaacgctcac gggatgccgt tgactttgcc gaccaggtgg cgctggcagc ccggttggcg
     4021 gtggggttcc ccgacatcgg ggccggcgag cgggcccgat tccgggtcgt gctgctcgat
     4081 gagttccagg acacctccga ggcccaactc gttctgctgc ggtcgctctt cgcggccggg
     4141 gacagcgggg tcccggtgac agccgtcggc gaccccaacc agtcgatcta cggctggcgc
     4201 ggagcgagcg ccacgaccct cacccgtttc ccggccgagt tcaccgatgg gtcgcccact
     4261 cctgtgcggc aattggcgac gacctggcgc aacgacgcat ccatcctcaa ggtggccaac
     4321 cacctcgcgg caccccttcg ggcgtccgcg cgggtcgatg tggcacccct cgcggtccgt
     4381 cccgacgcgg gcgtcgggga cgtcgtcgtc gcccgcgtcg gcactcacgt cgaggaggcg
     4441 gcatacctcg ccgactggct ggccggcgcg acgagccgcg cgggtacgac ggcagcagtg
     4501 ttgtgccgca agcgatccca gttcggtccg gtgatggagg cgctcgacgc cgccggcgtt
     4561 ccctacgagg tcgtcggtct cggcgggttg ctgctgaccc ccgagatcct cgacctggtc
     4621 tccgcgttgc gcgcgatcgc tgacccgagc cgcggtgatg cgctcatgcg tctgctcacc
     4681 ggcccgatgt gccggttggg ggccgccgac ctcgacgggc tgcacgagtg ggcgaggttc
     4741 cgccagaagg tcgtcaaggc cgagtcgcag gggcggctgg cgatcgacct cgacccggga
     4801 catcgtgacg aggacgacgg cgtcgagggt cccggtgatg cgctcgtgga cctcgcgccc
     4861 gaggtgttgg acgagccgag catcattgag gcgctcgacg atctgccgcc cgaggtgtgg
     4921 cgtggcaccg agggtcagta cgtcggtgcg ggcgcgctgg ttcggctcaa ggggctgtcg
     4981 accgccttgc ggcggctacg acggctcggt gcgctgccgc taccggacct tgtcggtgag
     5041 gccgaacgag cgctcgggct ggacgtcgag gtgctctcgc gcccgggtta cacgcccgac
     5101 accgcgcggg cccacctgga tgcctttgcc gacgtcgctg ccgacttcgc gtcgtccgcc
     5161 gaccgcccca ccctcatcgg cttcctcgcc tggctcgatg ctgccgtcgc acaggagcgc
     5221 ggcctggaac gtgccaccct cgagccgtcc gcggaggccg tgcagatcct caccattcac
     5281 gctgccaagg gcttggaatg ggacgtcgtg gcagtgccgg gcctcgtgga gggcaccttc
     5341 cctgcccgcg aaggctcgcc cacgccgaag cacaacggtg ctcgctgggt ggttcccgac
     5401 ccgaccccgg gaggctggct cgtcgggctc gcgggggttc cgttcgcttt gcgcggcgac
     5461 cgggacgggt tgccgacgct gcaggtggag ggcgcttccg acagtaaggc tctcagcgaa
     5521 cgggtcgcgg ccttccgggt cgaggagggg ctgcgcgatc tcgatgagga gcgccgtctg
     5581 gcctacgtcg cggtcactcg agcccggagg cgactgctgc tcaccgcgag cgtgtggacc
     5641 aacacccagc ggcacaaggt cacctccagg ttcctccgtg aggtcgttcg ggctcgtgac
     5701 gagctcggtg tccgggtcgc gcgctgggat gcgatgcccg acccggctgc ggtcggcacc
     5761 aacccggtcg ctgacgagac ggtgcgcgtg ttgtggccga ctccgcccga tcagagnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngact ggggaggggg tggcggcata
     5941 cccgcgcgcg gccctgctcg ccccccgagg tggcggcgcg gacgatccgt atgccgcgga
     6001 tgtcgacctg ctgctcgccg agcgggcgtt ggcccgcgag acgggccccc aggtcgtcga
     6061 ggtgccacgg cacctgtcag cgtcggacat cgtgcggctg gccgaggatc ctgagcgatt
     6121 tgctggcgag cgccgccgac cgatgcctgc cgagcccgcg cttgaggccc ggcgtgggac
     6181 ggccttccac gcgtgggtgg agcagcacta cgcgcgggcg accttgctcg attccgccga
     6241 gctccccggt gccgccgacg acgatccggg ctccgacgcc gagttgccgc agctcaagga
     6301 gaccttcctg gccagcgagt gggcgtggcg cacgccgata gatgtcgaag tggccatcga
     6361 gacggtgatc gacggcatcg ctgtgcgcgg gcgcatcgac gcggtgttcg ggcgagcgga
     6421 cggcggggtc acggtcgtcg actggaagac cgggccacaa ccgtcggggg cggacgccgc
     6481 gcatcgggcg ttgcaggtgg gtgcgtacgc gctggcctat gcgcggcttc gtggattgtt
     6541 gcccgatcag gtggatgcgg cgttctacta cgcgcggacg gggacgacgg ttcggccgac
     6601 gttgccggac gaggccgagc tcatccgctt gttggggtcg atcgccgact aggccggctc
     6661 gtctggcgtt gccggcacgg attgctgttt tggcgcgtct ggcgttgccg gcgcgtcagg
     6721 ccttgacggc gaagcagggc cgtgggcggt agggatctcc tcggtggcat cgaagagggt
     6781 ccgcgggtcc atcgccacgg tgtcatcgag agccgcagcg tcatcgagag tcactgtgcc
     6841 atcgagagtg accgagtcat cgtgagtcac cgagtcatcg tgagccgcag tgccatgaag
     6901 gggcgccgtg tcatcgaaga gagtgtccgg gtcgacggcc acggtgtctt cacccgagcc
     6961 ggacccgttc gcggcggccg ggcttgccgc tgccaccgtc ttcggaacga tgacctcccg
     7021 ggggaccacg gagaccggct ccggcgcctt caagtcggcg tcggtgaccc gccgctccaa
     7081 ctcgacaagg cgtcgggcca tcgcccgcac ccggtccgga tcgccgaggg tcaccgcggt
     7141 gagcaggctg gtgagctgcg cgaactcagc ggccagccgc gcccgtcggc gcagatgggg
     7201 gtcggggcgc tcgatccgtg agttggcata ggcctccaac accgactcga cggcagccgg
     7261 cccagccaga gtgacgatgg cggcgaagtc gtcggcaggg tcggccacct tggcgtcctc
     7321 ccaaccgacg acggcccgaa cccgccccgt ggctgcgtct tcatcggagt cgaaccccgc
     7381 cagcacctgc gacccagtca acccaccgtg gaccggtgcc ggagcgaagc gccacaaggt
     7441 gacgtcgtca agggccccct cccagcggga cagcaaggcc gtggggacat gccccgtggc
     7501 ggcggctcga tcaaggtcgg ccaggtgcct ggtgcgatac gagtcggcgt cgtaggacgg
     7561 cataccagcc tcgtcgaaga cgccccgatc gacgttgtgg atcgccgcga tggcccgccc
     7621 gaggtcagcg gccagcccgg ggcctgccgg aagcgcgtcg aacgccaacg gtcggcccgg
     7681 caagacggga tagacgccgg cccgtccgtc ggggagcgcg gcgaaaccct tgggcaccgg
     7741 cacctggaac ggcaaccgcc gggccagcag ggcagccagc gccgcggccg actccagccg
     7801 cgctccggcc gcaggcgtcc gcgggcagcg aacaaaccac ggccggtcct gctcatcgat
     7861 gacgatcgct gcgtcgaact caccggccgg gccgggcgcc accgcgcgcg ccgagacggg
     7921 atcgagcccc ggcacggccg cgctggcgag ggcggcgagg aacgccgggc ttcggctctc
     7981 gggactcacg ctcacacggt atgccgtgaa tgtcgccggt gcgagacgcc tcgcccgtgc
     8041 ttgactgctc ggatggaaca gacgccgggc ctgcggggcc tgctgctctc gcatggcgga
     8101 ccggatcggg ccgctcatct gcgcgaggac gcgggcctgc tcgaccgact cctggcggac
     8161 gcggggaccc gggtggtcgc ggttcgtggt gatcgagtgc tcgttgcacc agtgccggcg
     8221 cactcgatgg ccccaacggc tgacccctcg ccggccccgg ccgatggcgc ggcgggagcg
     8281 ctcggcttgg tgcttcgctc accctccgca gccgatggcg ggttgctcgc ggtcttcctc
     8341 ggtctgcacc cgggcgagga cggtgagcgg acggcatacc tgggagtcgt ggaggcaccc
     8401 acggacggcg acggcgacgc gggctggcag accctgcgag cggccggggc gttgctgccc
     8461 gacctggacg cgagcctggc cgcgaccctg ctcgcgctgg cgaactggca tcgcagtcac
     8521 gagcgctgct cgcgctgcgg ggcgccgagc gagccgacat ccgccggctg ggtgcggcgg
     8581 tgcacgcgcg acggcagcca gcacttcccg cgcaccgatc cctcggtgat catgtcggtc
     8641 atcgatgatg acgggcgatt gctgttgggg cgcggcgtga cgtggccggc gaaccagttc
     8701 tccgtgctcg ccgggttcgt cgagccggga gagtcgctgg aggcggccgt ggcgcgggag
     8761 gtcctggagg agtccggcgt cgtggtgacc gaggtgagct accttggcag ccagccgtgg
     8821 ccgttcccgt cctcactgat gctcggcttc acggcccgcg cggtgaccac tgcactcacc
     8881 cacgacccga ccgagatggc cgaggtgcgc tgggtgacgc gggcggagta tgccgcgctg
     8941 ctgcggtcgg gggcgatccg ggtgccgggc agcatctcga tcgcccggcg aatcatcgaa
     9001 cggtggctcg gtgccgatct tgaggtggcg gcggggcacg cggtcatcga gggctggcgc
     9061 ccgacgacct aggcgagccg agccttgacc tgcccaatcg acgggttggt ggcggcgctg
     9121 ccgtccggaa agatcaaggt cggcacggtc tggttgccac cgttgacgga catgacgacg
     9181 tcagcggcct cgggttgaac ctcgatgtcg acctcggtga aggcgatgcc ctcgcgctcc
     9241 atctgactct tgaggcgacg gcagtagcca caccacgttg tcgtgaacat cgtcactgtg
     9301 ccaggcgcgg gcgtgaactc ggacatgagg ctcctcgtcg gaatgtggtt gacacgacaa
     9361 ccacggcgag cgcgcccgtg ttccgggccg gatcgagccg gagtgtaagc gctacccccg
     9421 cgcgtcgcca aggggtgagg cacgggtgaa cgcgagatga acggcatacg agacgccccg
     9481 cgacagggga ccaccgggag tggaacaatg cgcgccatgg cgaagcgaac cgcaaacggg
     9541 accctgaagt tggacaccgc gacctatgag aaggagttgt tccgactcca agccgagttg
     9601 gtgacgatgc agcaatgggt catcagcact ggcagccgtc ttgtcgtcct ttttgagggg
     9661 cgcgacgcgg caggcaaggg ctcgtcgatc aagcgcatta cggagtacct caaccctcgg
     9721 caggcgcgcg ttgcggctct gccggcgccg accgagcggc agaagaccca gtggtacttc
     9781 cagcggtacg tcgagcatct tccggccgcc ggcgagatcg tgctctttga ccgctcctgg
     9841 tacaaccggg ccggtgtcga gcgggtcatg ggcttctgct ccgacgtcga atatgcccgg
     9901 ttcctgcgcc aggccccgtc gttcgagcgg atgctcattg acgacggcgt catgctgcgc
     9961 aagtactggt tctcggtgag cgacgtcgag caggaggctc ggttccgttc gcgggccaac
    10021 gacccgatgc gccggtggaa gctctcgccg atggacatgc agtcgatcac ccggtgggag
    10081 gactacagcc gggccaagga cacgatgatg cagttcaccg acctgtccga ctcgccgtgg
    10141 tacaccgtcg agtccgacga caagaagacc tcgcggatca acgtcatcca gcacctgctc
    10201 tcgacgattc cctacgagga ccagcagccc aaggcggtcg agatcccggg ccgtccggtt
    10261 gccgtcggct atcaccggcc gccgcgcgag tccaaccgct acgtcccgga ccacgcgcac
    10321 acgctgccga tcctcgtcgt cgagcccaag aagggcgcgg ccaaggccaa gggcaagaag
    10381 gccggcgacg ccaagggcgc agacaagaag tcggcaggca agaaggccgc tgctgaggcc
    10441 tccgcgccca caaccggtcc ggtcgaggtg acggcagcgg tcgtcgaggc tccggttgtc
    10501 gagtcggcgg ctgtcgagtc ggcggcgccc gaggtcatcg aggccccggt ggcggaggct
    10561 gccgtggagt cgaccgagaa ctgaccgcgg gcacgcacgg actggagcgg gcggcatacc
    10621 cctggggtgt gccgcccgct caccgttgta gcccgcccga ggggtgggtg gggccagcag
    10681 ccagcgggcc gggctccgtg aacaccgacc agcagatggg gttgcgtcgg gcctggtcgt
    10741 cgaggacgag cgcccggatc gggtcaggca gttggtcgag ctgccactgg cactcgcgac
    10801 gggcggcggc ctcgccgtcg tcaccagccg cggcggcggc ctgggctgcc ttgatcgcgt
    10861 aggcggctgc gccgaggtcg tgttcgggga catgggggat gcaggctgcc tggcccgcgg
    10921 catacgccgc gaaacgggct gccccgctca acggtctggc ggcccccatc gcgtgcccac
    10981 cggctgccct ggactgcatc atggcgagct cgcccgcagc ccaggcccga gcgccggcga
    11041 tcgcgtgccg gggccggaga tcactaggac attcggcctc gaacaggtca aggacgtgct
    11101 cggcacaggc ggctgcccac aacgccagga ggtgatgatc cgggtcggtc aggagcccgc
    11161 cacgacggat cgtcacgagg cgagggtctc gcaccttcgg gaggatcacg ggtctggtcc
    11221 cttgcggacg ggtggcgggt gtcaggtcga cgtcagggtc ggcggcgcgc tgacaccgct
    11281 cggaagagcg gcgtggtgca aaccctagga gaactgtgac ctgtttgtga cctcgacgct
    11341 tgtggataag cgggctgagc ggcgggggag ggtcaggccc cctggctagt ttgaggtcat
    11401 gagcagcacg tcggccaccc tgtcgggcgg tgcgtcggtc gccgaccgac gggccgcctt
    11461 cgtggctgcg cggtcggccc tcgacgggtt gaccgggctg ctgtgccagg tcgccggtgc
    11521 cgagttgggc cacctggtgg gtgagctcga tgcactggcg tcccgggctg cgggggcggt
    11581 ggtcgccgtg gtcgcggagg ctgactcaag aggtgagatt tcggccagcc aggcggggac
    11641 gagcactcag tgggtggctc gacacgctcc ctcactggcc gccgaagggg cgggtcaagt
    11701 ggcccggttg gtgcaggagt gtcgtcttcc ggagctggcg actgtgcgcg cggcggtcga
    11761 gggcggcacg gtttcggtgg gtgtggggct cggcgtggtg tccgagttcc aggcattgcg
    11821 acccatgctg cgcaacgagg cgcgacccac cgtgctcgac gggctcgtgc agatgggtgc
    11881 tgagcaaggg cgtcggggcg tccgtcggct gcggccggcg ctgttggcgc agttcggcga
    11941 cccaggagtg ttccaggaag tgcaggatgc tgccgggcgt ctggtcgcac tggaccgcgc
    12001 ggtcggcgac gtcctcggcg cgttcaccta tcggctggtc gtcgacgccc agggcaaggc
    12061 ggcgttggag gccgcgatcg gcccgctctc ggctcccgtg cctgggccag acggagcccg
    12121 cgatccgcgt ccacccgagc gccgtcgcgg cgaggccctc atcgaggtat gccgccgcgc
    12181 gacggctgcg ggcggcatac ctcctagcgg ggtcaagtcg acgctgatgg tgacgatgcc
    12241 cttcgccgac ctgagcaacc gactgggggc gggggaggtg ctggggtcga gcgaggccgg
    12301 agagctgatt gcgcccgaga cggtgcggaa gctggcctgc gatgccgggg tgatccctgt
    12361 ggtgctcggc ggcgagggcg aagtgcttga cctcgggcgg gcgaccaggc tcttcactgc
    12421 tggccagacg gcggcgttgt ggctgcgcga tcgagtgtgt tcgttcccgg aatgttcgat
    12481 ccccgcgcat tggtgcgatg cccaccacct gatccactgg ctcgacggcg gcgcatcgca
    12541 gttgtccaac gcggccctgc tgtgcgggcg acatcacacg gtggtgcacc gcgaccgact
    12601 gttcgggcag gtgacgtccg atggagtcga ctgggatcga cgcctgggtt cctacgatcg
    12661 cgcgctcgcg gcgccgggtc acgggggcaa ggcgggcgag gccgccgggc ccgatcctcc
    12721 cgctgcgtga cgggagcggg gcggcacacc cggtggtatg ccgccccgcc cctggctctg
    12781 tgtcggcgcc ggtcagtcgg ctgccgagtc gggtgtcaag gccggtcagt ccttcgggcc
    12841 gcctgcgacg tagatgacct ggccgttgac gaaaccggcc tcgtcgctgg tgaggaacga
    12901 gacggtggcg gcgatgtcct cgggctgacc gacgcgggcg accgggatct ccttggccga
    12961 gaacgcgatg aagtcctcga acgacacccc gaggcggtca gccgtcgcct tggtcatctc
    13021 ggtctggatg aaccccgggg cgatcgcgtt gcacgtcacg ccgaacttgc ccaactcgat
    13081 cgccagcgtc ttggtgaagc cctgcatgcc ggccttggct gcggcgtagt tggcctgccc
    13141 ccggttgccc tgcgccgacg tggacgacaa gttgacgatg cgcccgaacg tcgcctcggt
    13201 catgtacttc tggcaggcct tggacatgag gaacgcgccg cgcaggtgca ctgacagcac
    13261 gctgtcccag tcttcctcgg tcatcttgaa caggaggttg tcgcgggtga tccccgcgtt
    13321 gttcacgagg atcgtcgggc ctccgagctc ggcggcaacc cgctcgacac cagcagcgac
    13381 cgacgcgctg tcggcgacgt tgacgcccac tgccagagcg cgaccgcctg cggcgacgat
    13441 ctcggaaacc gttgcagcgc aagcagcttc gtcaaggtca agcactgcaa ccgcgtgtcc
    13501 gtcggcggcg agccgcttgg cggtggccgc gccgattccg cgggctgctc cggtgacgat
    13561 ggcggtgcgg ggggtggtgc tcacgggaga ctcctcgagt ggacgggtcg cgcgcacggc
    13621 cggcgcgtgt cgcttcgagc ctacgacggc acggtatgcc gcgggcaggc cgtctctgcc
    13681 tcgctgaggg tggggtgcat caccccttga gccgcagcat cccttcctgg gcgatcgagg
    13741 cgagcaaggt gccgtccgcg gcatacatcc ggcccaggcc gagcccgcgc ccgccggagg
    13801 ccgacggaga gtggcccgca tgtagaaccc attcgtccgc gcggcccggc cggtggaacc
    13861 acatggcgtg gtcgaggctc gcttggcgca gtcgcggatc ggaccacgcc gtcccatggc
    13921 ggcgcagcac cgattcgagg atcgaatagt ccgaggcgta ggcgaggaac gccgcgtgga
    13981 tgatcggatc gtcgggcatc gaccgccgcg aacgtagcca gacgccctgt cgcgcgacct
    14041 gctccggccg cgcccccgcg taaaggtgcc cttcgacgtg ccgcagctcg atggggccgt
    14101 cgaggatgca gacgacggca tacggctcgg ggacaccggc cagagcctcg gcaacgttgg
    14161 gcaggctctc ggcgctcggc gccgggggca tcggctcggc gtggtccaac cctggcgacg
    14221 cctcctggaa cgacgcggtc acgaccgcca acagcgtgcc gtgctgcagc gccgacaccc
    14281 gccgggtcga gaaggaccgg ccgtccgaga gcggctcgac aacgtagtgg acctcgtcgg
    14341 tgtcggcccc cgagaccagg aaggtcgtgt gggtcgagtg cagaggtcgc ccggcaccgg
    14401 ccgggtcggc ggcgaccgtg cgcccggcgg cgatgatcgc ctgggcgagc aactgcccgc
    14461 cgaacgtccg ctggtagcgc tgcttctggc tcggtcccgc gaacacccgc gccacccgcg
    14521 acccggcgcc cacctcgacg gcgaggtcag cgacagcggc ggtcgccgtg gccgtggccg
    14581 tggcgatgtc ggctgtaacg gctgccgtgg gactctcgga cggggaggcg accgcccacc
    14641 ccgtgtgcac ggtgcccacc tcgcgaaggt cgaggatgtc gaggaggtcg ctgacggcat
    14701 acgcagccgg ggtgtcagtc acgaccccga gggtatgccg cccgctcacc actgcgcacg
    14761 ggtcaccact gcgcacgggg ctgctcaccc ctccttgagg cggagcatgc cttcctgggc
    14821 caccgatgcc atgagtgccc cgtcggcgct gaacatccgc ccaactccca aaccccgacc
    14881 accagaggcc gatgggctgg acatcgtgta gagcacccag tcatcggcct tgggcgcgcg
    14941 gtggaaccac atcgcgtgat cgaggctggc cgggcgcagg cgtgggtcct gccaagcgac
    15001 cccatggcgg cgcagcactg gttcgagcat ggggtagtcg gaggcgtagg cgagcaccgc
    15061 tgcgtggatc accgggtcgt cgggcagttc ggcgcgggcc ttgagccaca cgctttggcg
    15121 cgccgctcgt tgccgcccgg gcttgacata gatgttgcct tcgacgtgcc gcaactcgat
    15181 gggtcggcga gcggcgaaga actgcgcggc cgacgggtcg aggtgctcac ccagggcctc
    15241 ggccagcgtg gggatgtcgt cagggtcagg cgcgggcggc atcggatcct gatggtccat
    15301 gccgtccgcc ggctcttgga acgagcaccc catcgacagg atggtctggc cgtactgcac
    15361 cgcttggacc cgccgcgtcg agaacgacgc cccgtcccgc actcgttcga cgacgaaccg
    15421 gatcggatgg ttgtcgtcgc cgccgcgcac gaaatacgcg tgcagcgagt ggatcgggcg
    15481 ggctacgccg tcgaccggct caaccgtccg ggccgctgcg acgagggact gagccagcac
    15541 ctggcctcca aacacccgcc catgcgcctg tttctggctg cgcccgacga agacgatcgg
    15601 ggtcgactcg cccagatcgg tctcctcgtc accgggcgcg ccctcgacga cgatccgtgc
    15661 ctcgcccaag caggtgaggt caagaacgtc gagcaggtca gcaacagagt cggcggccga
    15721 gtcgggcagt ctcatgagcg tgaggctatc cctggggact gacaggatgg cgcccatgcc
    15781 cgaccccatc ttggccagca cgttggccag cacgttggcc agcacgttgg ccagcgcgcc
    15841 ggccgccaca ccggccgccg ccactgtggc acccgatccg accgaggtgc tcgtcccgtt
    15901 gcggtggtcg gacatggacg cctacggcca tgtcaacaac acgcagttcc tccggctgct
    15961 cgaagacgcc cgcgtggcag ccctgccccg atggttcccg gagggcctgg cgctgctcga
    16021 acggggcatt ctcgtcgttc gtcacgagat cgagtaccgc gcgcaactgg actatcgccc
    16081 ggagccggtc gccatcgacc tctgggtgac ccgcatcggg ggagcaggct acgacctggc
    16141 ctacgtcgtc cgtgaccccc acggggtggg ctccggcacg gtctacgccg tcgccgagac
    16201 gtcccttgcc ctgttcgact tcgccgccaa caccccgcgc cgggcgactg ccgtggagcg
    16261 cgagtgcctc accgcccacc tcggtgcccc cgcgccgctg cgtaagcgca gatgagcacg
    16321 cgatgaccga tctcgtcttc cccgatcagc aggggtatgc cgatctggcc actctcgtgc
    16381 gtcgggcccg ccaggccgat cccgacgcgg ccgtccgcct gcaggtcacc ggtgcggtcc
    16441 tcagggtgtg ggtgggggtc cttccgggca cgggactgct cgccgacgga gccgctgtcg
    16501 gcgtccgggg catcgagttg gcgaatgagg atgcgggcgg ctccacgtca gaggccggcg
    16561 cgctcgacct cgtctgcgcc tcggccgcgc tgagcgaccg gttcgcctac gacggtgtcg
    16621 gcgcgagcag gctgtcggtc cctccggcca gcacgtatgc cgcgtgggcc ggcgccctcc
    16681 cggcggtcgc ggggtggtcg tcgattggag ccatctccgc gcaggcactc atcgacgcgg
    16741 cccgggccgg catcgccgag atcgccgaag gcgtcgatgc gcgacagacg ggtgccgccg
    16801 ccgggtctcg ggcggtccag gagttgcgcc gccgggtctg ggcgcgggcc gtcccgatcc
    16861 ccgcaggggt cgacgacggg cccgtcaacg gtgccgatga ccagcggcca accgggtttg
    16921 agggcgtccc cgcaggggcg gcattcgcgg catacgtgct cgggttcctt ccggagccga
    16981 gcgcgacggt gcgggtatgg cgaagcgggc gctgggttcg gctacagact gcggtggggc
    17041 acgtcgtcgc ccgaccgtga ggtctccgat gcgcagcggg cggccgagac gatccccagc
    17101 gcgcccaagg cagccagccc aatccacacg atgacgaagc cgcggtgggc acctgtcgtg
    17161 tgccagatcc cgaacgccgc cgtcgcggca gcaatgccga ctacggtccc gagtacgtcg
    17221 gctagttgca acgcggccga cgcccgcccg tgttcgtggt cggggctcag ctcaaggacc
    17281 agccccgaca gcgtcgttga ccccaggccc atgcccaacc cgccgacggc gacggccgcc
    17341 gcaaaagtcc acaccggtgc gtgtcccagg gtcaggccga cgaacgcggc ccacccgacc
    17401 gcaagcaggg atgcgcccaa gacgaccaag ccggtccgct cgtcgatgcc ggaccgtcgt
    17461 gagcgaccct gcgcccagga cccggcactc cagccgagcg agccggccgc aagaacgagc
    17521 ccggccgagg tggcgtcaag ccccttctcc tgggtgagca tgagcggcag gtaggcgaag
    17581 gcgccgagga acgacccgtt gagcaatgcc ctggtgagga ccaccgaggg caggccgcgg
    17641 gcgctgcgga acgtcccggg aggcagcaac ttgggcgcag cgatggcgac ggcgatcaga
    17701 gctaggacgg cggccgcagt caggaacgac acggcagggg gccgttcgtg gatcgccaac
    17761 tgcaccacgc cggccgcgcc ggccacgacg gcgcccagac gggccgtgcg tcgatgttgg
    17821 cgtcgctcgt cggcagggtc atcggcaggg tcggcggctt cgcggacggc tgggtcggca
    17881 actggggcgt cggccgtggc gtccaatgcg ccgtcgtcga gtgcgccgac gtggcccatc
    17941 gcgaccgctg catccgccag tcgtcgagca tcgctcgcgc tcgcgctcgc gnnnnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcggtgcgg gcggcgcggt tcacgtcggc
    18121 cacgcccctc gggcggcgag cacgtcccgc agcacgtcga tccggtcact gacgatggcg
    18181 tcgacaccca ggtcgaacaa ccggaccatt tcgtcggggt cgtcgatggt ccagacatgc
    18241 acctgcagcc caaaccggtg cgccgccgca ataagccctg gcgtcaccaa cgggaacgtc
    18301 cgcccgcgca cctcgtgcgt cgtggggatc tgcaggacct gtccgggtga gtgcgccagc
    18361 gtggacagcc ggccgggcgc atatctcagg gcagccgtgc cgagttggcc ggcagcggtg
    18421 gccacgcggg ctccggccag gcgtcggaac gcgtgcaggc gacgttctcc gaacgagccc
    18481 acacacaccc ggtcgtatgc cgcgtgcctg cggatcgtct cccacagcgg caccaccgcg
    18541 ccgggggcct tgatgtcgat gttgatccgc gcctcgggga aggcgtcgag caggtcgtcg
    18601 aggctgggga tcggttggct gccagcgatc cgggcggccg cgacctcgct ccacggcaac
    18661 tcggcgatgg cgcccgcccg gtcggtcacg cggtcgagat gctcgtcatg gaaggccacc
    18721 aaccgcccgt cggcggtggc gtggacgtcg gtctcgagga agcgatagcc caggtcgact
    18781 gccgtggcga acgcggccag ggtgttctcg atcccgacgt tggcagggtg actcgccccg
    18841 cctcggtggg ccagcccgaa cggcggcggc gcgaagtagg ggtagtcggc cgcgctcacg
    18901 aggaacaccc tgccacgagg cggggctacc ggccgccgaa catgtcgtcc atgagcacct
    18961 gggcgaactc gggcgcggtg cggccttcct ggacgacggc ggtgacgttg gcgacgatcg
    19021 gcatgtcgac cccgtgtgac cgcgcgagtg cgaggatcgc cccgcaggaa gagacccctt
    19081 ccgcggtctg cttcgtgacg gcgacgacct ccgccaccgt catgccggtg ccgaggttcc
    19141 ggccgaacgt cgagttgcgc gaatgcggcg acaaacaggt gacgaccagg tctccgaccc
    19201 cggccaaacc ggcgaaggtc gccgggtgcg ctcccatggc ttcgccgagc aatcgcatct
    19261 cggtcagccc gcgcgtgatg acggcagcgg tgacggagtg accgaggccc aggccttccg
    19321 tcatccccga ggccaccgcg atgacgttct tgacgcttcc gccgagctcg gccccgacga
    19381 cgtcgtcatg gacgtacggc cggaacgccg gggtggcgca ggcgtcggcg acccggtggg
    19441 cggtggcgag cgaggcgctc gcgaccgtcg tcgcggccgg ctcgcgtgcg gcgatctccc
    19501 tggcgatgtt ggggccgctc acggcaacga tctgctcggg cgcgaggtcg aggcactcgc
    19561 cgacgacctc ggtcatccgc ttggacgtgc ccttctccag gcctttcatc agcgacacca
    19621 ggacgatccc cggcggcagc aacggccccc actcggcgag gttggcccgc aaggtctggg
    19681 ccggcacggc cagcacgccg atgccggcgc cagccagagc ttcggcgggg tcggtcgtgg
    19741 ccgtcaggct gtcgggcagg cgcaggtcgg gcaggtagcg cacgctcgcg tgatggcgct
    19801 ggatgtccgc gacctcgtcc tcgaacttcc cccacatccg cacctcgcac ccggcctcag
    19861 ccagcacgga cgcgaacgcc gtcccccaac taccggtccc gaagaccgct actcgctctg
    19921 acgtcgtcgt cgccgcactc acggcgcaag cgtggcatgg cgaccgcgcc gaccgcgaga
    19981 gggacgtgaa tcggaaggcg cacgaacgac cgactgctga caggcacccg cgggaacgtg
    20041 acaccatgag cggacgaggc cgacgccacc cgctcggccg atgacgtgac cctcgacgag
    20101 gaggaaccct ccgatggatg ccctgttccg tgtgccggcc cccgccaacg agccggtcct
    20161 gggatatgcg ccaggtagct ccgagcgcgc gacgctcgaa ggcgaactcg cccggatcgg
    20221 cggcgagcag atcgacctgc cgagcacgat cggtggccgc aaggtcatgg ggggcggccg
    20281 gaccggcaag gtcgtgcaac cgcacgccca ccgcaaggtc ctcgggacga tgaagctcgt
    20341 caccccgatg gaggcccaga gcgcgatcga cgccgccaag gccgccgccc cgatgtggcg
    20401 cgatatgccg ttcgccgacc gggccgccat cctgctcaag gctgccgagc tgctctccgg
    20461 gccgtggcgg gcaacgctca atgcggcgac gatgctcggg cagagcaaga ccgccccgca
    20521 ggctgagatc gactcggcct gcgagctcat cgacttctgg cggatcaacg tccacttcgc
    20581 ccagcagatc cttgccgagc agccgccgct caactccaag ggtgtgtgga accgcagcga
    20641 ctaccgatcg ctcgaaggct tcgtctacgc gatcacgccg ttcaacttca cggcgatcgg
    20701 cggcaacctg ccgaccgcgc ccgcactcat gggaaacacc gtcgtctgga agccggcccg
    20761 cacgcagcaa ctggccgccc actacacgat gctcatcctc gaagccgcgg ggctgcctcc
    20821 cggcgtcatc aacctggtca cctccgatgg cgcgaccctg tccaacgcgg tcctcgccga
    20881 ccgcgacttc gccggcatcc acttcaccgg gtcgaccggc gtcttccact cgatgtggga
    20941 gcaggtcggg gccaatatcg gccgctacaa gacctacccg cgcctggtcg gtgagaccgg
    21001 cggtaaggac ttcgtcgtcg cgcacccgtc ggcggacatc gacgtgctgc gcaccgcgct
    21061 cgtccgtgga gccttcgagt tccagggcca gaagtgttcg gccgcctcgc gcgcctatgt
    21121 cccggcgtcc atttggaagc gcctgagcaa ggacctggcc gacgaggtca actcgctcac
    21181 ccagggcgac gtcaccgact tcggcaactt catgggggcg gtcatcgacg gcggcgcgtt
    21241 cgccgaccac aaggccgcca tcgaccgcgc tcacgcgact cccggcgtgt cagtcgtcgc
    21301 cggtggcacc tatgacgact ccgagggctt cttcgtgcgg cccacggtcc tcaagtgtga
    21361 cgacccgacc gacgagtccc tgcgcacgga gtacttcggc ccgatcctct cggtgcacgt
    21421 ctaccccgat cggtcctacg acaggatgct cgaccagatg gagtccttcg cgccctacgc
    21481 cctcaccggg tcgatcatcg cccaggaccg cgcagcgatc gtcgccgccg cccacaaact
    21541 caggtatgcc gcaggcaact tctacatcaa cgacaagccc accggggcgg tcgtcggcca
    21601 gcagcccttc ggcggcggcc gtgcgtccgg caccaacgac aaggccggtg ccgcacagaa
    21661 cctcatgcgc tggacctcgg tccgttcgat caaggagacg ttcgtcccgc cgacggacta
    21721 ccgctacccc tcgatgggtt gatcgacgcg gcttccccgc cgtgggctga ccaccaccat
    21781 caggtatgcc gctcgctcac ctaggcgcgc ggcatacctg gagggtcctg cgcgcggggg
    21841 ggagtcagtc ctggtcggtg tgaccgcgtt cggcgagcca ggccaggccg acgccgtact
    21901 ccttgtggat cccggagatc accgacggcg cggcggcgga ctcgaccttg ccgccgacga
    21961 acgggatgct cgccttgagg ttgccctcga tgatcacttc ggtgccgtca tcggcgggga
    22021 tgattcggat gtcacccttg agatggatcg gggctgagcc catcgagatc gacatctcgc
    22081 cgatgcgcgt cccgtgcggt cccggcgggc cgtagacgat cgtctcgacg atgtcgatgc
    22141 tctgcccgac catcgagcga gcgaagtcgg ggaagctgtt cgtcggcatc tcacggtggg
    22201 tgatgaccgt cgccccatca ctttggcggc tcaccgtcgc ggagtagctc atggcgtggg
    22261 tcgcctcgca cacccgggag tggaacgcct cgtcggtcat catgtcgaag acgacctcga
    22321 tggcggcggg gtagacgagg gtctcggtga tctgcatggt tcccgacctt agcgagccgc
    22381 cgcggatgcg cgcgccacct gctggctggc ctgggccagc tggcgggcga gttgggcccg
    22441 gccccgagct agtcgggacg ccagccgatc cgcctgggcc tgcacctgcg gacgctcgac
    22501 gccgcgtcgg gcggccacgt cggagccgat caggccaaag ggctccgtga ccgtccaatc
    22561 ggtgacgatc aatcccaggg ccgccgcgcg ctcctgcagc cgggagtatg ccgccgccgc
    22621 ttcggcgagt tcctgggcat ggacctgcag cgccgagccg atcgcatcga cctggtcggc
    22681 ggtggctgtg gccaactgcg ccaaagggct ggttgcaggc agggccgcga gatcgccgga
    22741 gagggtgtcc gccgtggtgc gcagcaggcc gcccagcctc gacaccgagc cggggtccgt
    22801 tccccgcagt gtcgtcacca tcggggtcga tccatgtccg acccggcgcg tcgtgtcgcg
    22861 tcgcgcccac caggccgcgc tacgtcaggc gtaacgcggt gctgcacgtc cgaaccggcg
    22921 tggtgctcct cggccccggc ccacgaaggg tcccgctgct gcgacagttg ggcgatcacc
    22981 cgcagcgcat ccacgacctg ctcgacgaac tcgtccaccc cgcgctgaac cagtgggtcg
    23041 ccgagcatcg ggtcgacggc gaacaggctc tcggacgcgt cgagggcgtg ctggcgcacc
    23101 tgcgcgagga catcggcagg cgcgccgtgg tcgcggcgtg tggaccggtg cgctgggcgc
    23161 tcaccatcgg gcacgaaccc accctagaca acctcgcccc taccccgttg cggttgtccc
    23221 caggccgaag gtcccgcaca gactcgaccc tttgtctcac ctgccgatac gctcggtgag
    23281 ggcacaacga cgtgcccatg acgacgagaa acgggttgtt caccatgacc atctcgcttg
    23341 agcagtctcg cggcaacgtg ctgcgcagcg ctgcgggctt ggctcagaag ggttccgggg
    23401 atcccgttga gcctttgctg acgcgctact accggcacgt gtccacggag gacctgctcg
    23461 cgcggtcccc ggaagacctg ttgggggcgg cgctgtcaca cctcgacctc gcccatgatc
    23521 ggccggtcgg cacggcgagc gtgctcgtcg acaaccccac ggtcgagtcg cagggctgga
    23581 gttcggggca cacggcgatc cagatcgtca tcgacgacat gccgttcctc gtcgactcgg
    23641 tgactatggc gctgacccgc cggggcctgg gcgtccacct gctcgttcac ccgcagctga
    23701 tcgtccgccg ggatgctcgt ggcgtgcttg aggaggtcct cgacgtcgac aaggctccgt
    23761 ctggcgaatt cggtgtcggg gtcgagtcgt ggatgcacat cgaggtcgac cggatgagca
    23821 acgcggccga tcgagactcg ctgtccgccg agttgcagag cctgctcggc aacgtccgcg
    23881 acgccgtcga ggactggccg aagatgcgtg agcggtgcgt cgagatcagc gacgggctgc
    23941 gcgccgcagc gccggcaggc atcgatggcc ccgaggtggc ccaaggggtg cgattcctcg
    24001 actggctggc cgccaaccac ttcaccttcc tcggttaccg ccagtacgtc ctcggcgacg
    24061 atggcggcga gacggtcctg cacccggtga ccggaacggg cctcgggctg ctgcgctacg
    24121 acgccgccgg atcgacggcc ttcgcgcgcc tcagcccgcc ggcccaggcc cgggcccgtg
    24181 accgcgagct gctcatcatc accaaggcga actctcgcgc cacggtccac cggcccgtct
    24241 acctcgacta cgtcggggtc aagaccttcg acgcagcggg cgacgtcacc ggcgagcacc
    24301 gcttcctcgg gctcttcgct gccgcggcat acacggcctc gatcatggac atccccatcg
    24361 tcggcgaccg ggcccgcgag gtcctggacc gggtcggcta cagcggtgac agccactccg
    24421 gaaaggacct cctccaggtc ctcgagagct acccccgcga cgagctcttc cagaccgacg
    24481 ccgacatgct ggccgagatc gccgagaccg tcgtccacct gcacgagcgg atccgcaccc
    24541 gactgttcct gcgcaaggac gtctacggcc ggttcatgtc agcgctggtc tacctcccgc
    24601 gggaccgcta caacacggcg gtccggttgc gcatggaagc gctgctgcgc aaggcgttcg
    24661 ccgccgacag cgtcgactac acgacccggg tgtccgagtc gccgctggcc caggtgcact
    24721 tcgttctgcg ggtgcccaag ggcgcccagg tgaaggacgt cgacgctgcg gcgttggagt
    24781 ccgagatcgt cgacgccacc cgcacgtggg acgaggacct cctggagagc gcccgctccg
    24841 agcacggcga ggagctcggc gcccggttcg cagcgctcta cggcaaagcc ttccccgagg
    24901 cctacaaaga ggacttcacc ccccgggttg ccatcgcgga cgtccgtcgg atcgaggtcc
    24961 tcggcagcga cgaagccgtc gagttcaaca tgtatgccga ggcgggcggg ccgcgcgacg
    25021 agcgccgctt caagctctac acgcgcagtc cgctctcgct gacccgggtg cttccggtct
    25081 tcacccacat gggggtcgag gtcaccgacg agcgtcccta cgtcctgcac cgggccgatg
    25141 gtgcccgggt ccacgtctat gacttcgggc tgcgggccac gtcggccgcg ttgtggggcg
    25201 agggcgccga acgcgagcac tgccgggcgc gcttccagga ggcgttcagc gcgatctggc
    25261 gcggtcgcgc cgagtccgac ggtttcaacg ccctcgtcct gcaggcgggg ctggcctggc
    25321 gggaggtcct cgtccttcga gcgttggcca agtacctgcg ccagactcag tcgaccttct
    25381 cccaggacta cgtcgagtcc gcgctgtcgg ccaacgcggg catcgcccgc gatctcgtcg
    25441 agctgttcgc gatccgcatg gaccccacac ggtatgccgg tctccccggt gccgagcgca
    25501 ccgatgccgc gcaggccgtg gtcgagcgga tcactgactc gcttgtccag gtgcagagcc
    25561 tggaccacga ccggatcgtc cgtgccctgc tggcgatcgt cctcgcggcg acccggacca
    25621 accactacca ggtcgacgcc gagggcgaac ccaagagcta cctgtcggtc aagctcgacc
    25681 cgaaggccct gcccgacctg cccgcgcctc gcccgatgtt cgagatctgg gtctacagcc
    25741 cccgggtcga gggcgttcac ctgcgcttcg gcaaggtcgc gcgtggcggt ctgcgctgga
    25801 gcgaccgacg cgaggacttc cgcaccgagg tgctcgggct ggtcaaggcc cagatggtga
    25861 agaacgccgt catcgtgccg accggctcca aggggtgctt cttcccgaag caacttcccg
    25921 agccggccgt cgatcgagac gcgtggctgg ccgaggggat cgcggcatac cagatcttca
    25981 tctccggtct gctggacgtg actgacaacc gcgtcggggg cgagatcgtg ccgcccgcca
    26041 acgtcgtgcg gcacgacgcc gacgactcct acctcgtggt ggccgccgac aagggcaccg
    26101 cgtccttctc cgacatcgcc aatgccaagg ctcaggacta cgggttctgg ctcgacgacg
    26161 cgttcgcctc cggcggctcg gcgggctacg accacaaggg catggggatc actgctcgcg
    26221 gagcctggga atcggtcaag cggcacttcc gcgagatggg ccacgacacc cagacgcagg
    26281 acttcacctg cgtcgggatc ggcgacatgt ccggcgacgt cttcggcaac ggcatgttgt
    26341 tgtctgaaca catccggctc gtcgcagcct tcgaccaccg gcacgtgttc atcgacccca
    26401 cccccgacgc cgcgagttcg ttcctggagc ggcaacggtt gttcgccctg ccgcgctcgt
    26461 cgtgggccga ctacgaccgt tcgctgatca gcgccggcgg aggcgtcttc gagcggtcgg
    26521 ccaaggccgt gccggtcagc cccgagatgg ccgcggcact ctcgatcgat cccacgatca
    26581 cggccctcac ccccgcggag ctgatgaagg cgatcctgct ggccaaggtc gacctgctgt
    26641 ggaacggcgg catcggcacc tacgtcaagt ccatcggcga gaccaacgcc gaggtgggcg
    26701 accgggccaa cgactcgatc cgcgtcaacg gccgcgcgct gcgctgccgg gtcgtcggcg
    26761 aaggcggcaa cctcgggtgc acccagttgg gccgaatcga ggccgctcag gcgggggtgc
    26821 gcatcaacac cgatgcgatc gacaactccg caggcgtcga taccagcgac catgaggtca
    26881 atatcaagat cctcctcggg cagttggtgc gtgacggcga tatgacgatc aagcagcgca
    26941 acacactgct ggcctcgatg accgatgagg tggcagctca ggtcctgcgg gacaactacg
    27001 agcagaacgt cctgcttggc aacgcccgcg ctcagacgct cgtgatgctg cccgtccacc
    27061 agcgcttcat ccactacctc gagcagaacg gcgacctcga ccggggcctg gagtatctgc
    27121 ccggcgacgc cgagattgtc ggtcggcaag ccgacgggcg gggcctgacc tcgccggagt
    27181 tctcggtcct cgtcgcctat tccaagctcg acctcaaggc ggccctgctg cccaccgcgc
    27241 tggctgacga cccgtggttc gcgggcaccc tggcggagta cttcccgacc cggctgcgca
    27301 acgagcacgc gaaccagctc gaccaacacc cgctcaagcg cgagatcatc atcaactcgg
    27361 tggtcaactc gttgatcaac cggggcggca tcacgttcgc cttccgagcg gccgaggaga
    27421 ccggagcaac gccggagcag gtcgctcgcg cgttcatcgt ttgccgcgag gtgtttgacc
    27481 tggccgggtt ctcggctgcc gtcgagtccc tcgacgcgac cctgccggcc aaggtgcaga
    27541 cggtgctcta cctggagttc cgtcgactgc tggaccgtgc cttgcgatgg ttcctggcca
    27601 accggcccgg caccatcgac gtgaccgagg agattgctcg gttcctcgcg ccggtccagg
    27661 agcacgccgc tctcgtcccc gaggtgctcc aggggtccga gcgtcgccgt ctggaccgcc
    27721 gggcggccga gttggccgca ctcggtgttc cggaggcgct ggcgatgcgc accgcgtcac
    27781 tcctcgacgt cttctcgatg ctcgacatcg tcgagatcgc gcaggccacg ggcacaccgt
    27841 cgcaggacgt cctgcgggtc tactaccacg cgtccgaacg gttcgggatc gacgacatgc
    27901 tcttccgggt gtcccagctc gcgcgggacg accggtggga ttccgccgcg cgtggtgcgt
    27961 tgcgcgatga cctgtatgcc gtgctcgacg ggatcacccg caacgtcctc gcgacgagcg
    28021 acccgaaggc cgacgccaag tcgcgcttca accaatgggc caaggccaac caggaagcgc
    28081 tcgaccgggc ccggtcgggg ctgcaagcga tcctgcgtct ggagtcggcc aacattgcgg
    28141 ccctctcggt ggcgttgcga acgctgcgtg ctttcgtcaa gacggccaca ccgacggacg
    28201 gctgacctgg gctcggcgct agagtcgcgg cgtggcgacc ctctccgagc tgatccagga
    28261 gcatgccgcc ctcccacagg tggaccagga gtggctgcgg ctgctcgtct cggactggca
    28321 gttgctctca gacctgtcct ttgccgacct cgtcctgtgg gtgcgcagtt cccctgatcg
    28381 ttggctcgcc gtcgcccaca tccggccgac gacggggcaa acggtctact tccgcgatca
    28441 ggtaggccgg gttgccggca ccagccgggt ggagttgctg gaggcggcat acctgcagcg
    28501 ccggatcgtc cgcgagcgcg acagcgagtg ggaccccgat gaccccgtgc gtgaggagtc
    28561 gatccccgtc gtgcgggccg gcggcgcgat cgcggtgctg acgcggcata ccaacctgtc
    28621 gatgctgcgc accccgagcc ggttggagct cacgtacctg gccactgctg acgcgctctc
    28681 gcggatggtc gccaacgggg agttccccca cccgaccgcg ccgacgggcc tgcgccgagg
    28741 ggctccccgc gtcggcgacg gagtgatccg gctggacacc gagggggtca tcacctacgc
    28801 gagccccaac tcggtgtcgg ccctgcaccg gctgggctat gtgggggaga tcctcggcac
    28861 gtcgctcgcc gaactcgtca cgtcggtctt gcacgacacc tcgccggtcg acgaaggtct
    28921 gccgctcgtc ctcacgggcc gccaaccgtg gcggaccgag gtgaccagtg ccggtcaggt
    28981 gatctcgatg cgggcgatcc cgctcagcga gtcggggcag cggatcgggg ccatcgtgct
    29041 ggcgcgcgac atcagcgagt tgcgccgccg tgagcaggaa ttgatgacga aggacgcgac
    29101 catccgggag atccaccacc gggtgaagaa caatctccaa tcggtggccg ccctgcttcg
    29161 tctgcaggcc cgccggatgc ctgatgcgca ggggcgggcg gccctcgaag aggcggtgcg
    29221 gcgggtcggc acgattgcgc tcgtccacga caccctcagc cacggcctgg acgagaccgt
    29281 cgagttcgac caggtcgctg atgtcacgat ccgcgcggtc gtcgaggtcg ctgccggcgg
    29341 gcggtcggtc gccgtgcgac ggaccgggtc gttcgggcgg gttcgggcag aggacgccac
    29401 ctcgctggcc atggttgtca gcgagttggt ccagaacgcc gccgaacacg gggtcgcgtc
    29461 gcagggcggg acgatcaacc tggaggtcaa gcgcaaggag ggcaaggacg agaccgacgt
    29521 cctgaccgtg accgtcaccg atgacggtgt gggtttgcct ggtgggcgac ttccggccgg
    29581 ggtcggcctg ggcacgcaga tcgtccaatc cctcgttcag gatctgcggg ggaagatctc
    29641 gtggggacct gccgaaccgc agggaacccg ggtccgcttc acggcacggt tacgttaacc
    29701 gctcgatcct ggcggtgcag ccaagccatc ggcgacagcg cgcaggtgac catgcgcagg
    29761 tgaccgtgcg caggtgataa acgcggcggc ggcggtggaa ctagccggcg cgacgggcgc
    29821 gggcgttgcg ccgcttgagc gcgcggcgct cgtcctcgga cagcccgccc cacacgccgg
    29881 cgtcctgacc ggactccagc gcccatcgca ggcaggtgtc gaggacttcg caacgacggc
    29941 agaccgcttt ggcctcttcg atctgcagaa tcgcgggccc ggtgttccca atggggaaga
    30001 acagctcggg gtcctcgtcg aggcaggcag cgcggtcgcg ccagtccatg tggatcgtgc
    30061 tcctagttcg aggtcgtgcc gtctggggtc aagcagatgt cgatgatgca ggttctgtcc
    30121 aggaccgtcg gggtccccca aatgatgagt gcaatgctga gacgaccgtg gatgcgggta
    30181 tcccccacgg aacccgcaag ccgtcatggc acccgcaact ctgggaggcc aacacgacat
    30241 tgtggtccaa aggactacct ttcggcaacg ggacatccat cacgtttgtt cccgaacggc
    30301 gctttcttcc tgccaaacgg tacgaatcgg agcatccagg ggcctacaac gctgcgcttc
    30361 cagcagtcga agggggtgcg accgcccgcc gccgcgccgt taggctcggg ccgtgagtac
    30421 tggtggcttc cgtccgaggg ttagttcggc gcggcgggtg gcggcgatca tctgcgcgat
    30481 tgaggcgctg gccatggccg gattcgggat ctacggtttg gttgaactcg cccggggggg
    30541 cggcaccggg accgctcggg tcatcaccga atcggtcctc atcctggcct tcgccgccgg
    30601 gatggccttg ttggcccggc tctggctcgg tcgctccggg tggccaggca ctcccaccgt
    30661 cgtctggcac gccctgctca tcccggtcgt cgtcagcatg gcgcaggccg gccagtggct
    30721 cgtcaccctc tcgctgtctg tcgccatcgt gctgtcgatc ggagcagcca cagtggctcg
    30781 cggttccggt agcgactgat cgaccgggtc ggtcagtcgc gcggcatacg cagcacttgc
    30841 gcgatccgac gggacgtgtc ggccaggatc tccacgatct cggcggtctg ctcagccgtg
    30901 agcccatgct ggcgccaggc ccggcgggcc tgctcgcgca cctcggatgc cgcgcgttcg
    30961 acgtccgtcc agtccgcccc ggcgaacgtc ccggcccctg acccgccacc tgacccgcca
    31021 cctgacccgg agccagatcg agaacctgac cgtggacctg acctgtcgct cgtcccgcca
    31081 tacgctcccg cgccggccgt tcggtccgtg ggcgactggc cccatcccgg gctcgatccc
    31141 tgtggccagc ccgcccccga ggtcgatccg gccggacgcg ccgtcgttcg cgcttcgcgc
    31201 gcagccgctt tcaactccgc ccgcagatcg gccgagcgac cctggacggt ggctcggacc
    31261 tgctcggcga ggcgacgcac cgattggtcg aggtcggctt ggaggtcttc cacctcccga
    31321 tgacgggcgc gcacctcggc gagccccgcc tcggtgatcc gataggtcgc cttgcggccc
    31381 tcgtcggtcc gctcgacgag cccttcctct tcgagtttgg ccaaccgggg atagaccgtg
    31441 ccggcgctgg gtgtgtagag cccgccaaac cgggtctcca ggtcctggat cacctcgtac
    31501 ccatgacgcg gtgcctcagc gagcaggacc aggaggtaga ggcgcagttg tccgtgggcg
    31561 aagacggggc tcacggggtg tcctgcccct cgtcgtcaat tgacgattcc cgcacctcgt
    31621 cgcgcgcggg cgcatcctgc ggtccgccgg gagacacttc gggagacctg tcctgtggcc
    31681 cagcatcccc actgccggac ggaccggccg ctggtgcggc ggcccgcagc aggatgacgt
    31741 cgcccgacac cgaattggcg cgcagggtca gtgactnnnn nnnnnnnnnn nnnnnnnnnn
    31801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31861 nnnnnnnnnn nnnnnngccc gcagcaggat gacgtcgccc gacaccgaat tggcgcgcag
    31921 ggtcagtgac tcgtcgccgc tgcgcagcgt tccgcgcccg ctcgaaccgc ccggtgcgca
    31981 gtcgccccag ccgccgggcg gcatactccc gctgctggca ccgtgcaggg actggccatc
    32041 gacgatgacc tggccggtcg cgctggtggc ggtgacggca taccccgaga agggcgcgcg
    32101 gatggtgacg tcgcccgtga ccgagttgga ctggacgtcc aagtgggtgt tgaccaggtc
    32161 gagcgcgacc tccccggaca cggtgttgat gcgagcccga gggatgtcgc tgctctgcac
    32221 cgtcaccgag ccagagacgg agttgatctt ggcgtcgccg acgaggtcgg cgcactcggt
    32281 ggccccgctg acggtgttga tcgtgctgcg gccctgcacg tcgctgatcg tcatctcgcc
    32341 ggacaccgtg ttggcggcca ggccgcggcg cagcccgctg acgacgatgg gtgcggtcac
    32401 ggtcgacacg gtcgcgcggg cggatgcggg caccgagatg ctgacatcgg cgtgctgacc
    32461 gctgacctgc tcgacgaggc gtcgcagggt gtccagcacc gtgctcccac catccttggt
    32521 gtgcatcacc ttgagggttc ggccatccca ggagacccgc agcggcaggc cctcgacgtt
    32581 cgagacttcg actcgggcgc catacccgtc gtcgtgcgtc acgacgttca cgcggccacc
    32641 gatgatgccc acgacgacct tctcgacccg ctcgcgatca ctgcccacgt cgatgacgcg
    32701 cggaccgtcg accaaccaat gttcggccat ggtgtctcct cgactccgat gcacgcgcta
    32761 tatcgcgtct tagagagaca cgttatatcg cgatagtccg catcgcaagg gccacgggaa
    32821 ccagacggtc gactgtcagc ccagttgcat ccacgcgttc cagccggcgt gcagcaccag
    32881 ccaggcgatc agcccgtagc ccgcctgccc cgggtgcacc ccgtcgccgg ccgcgaggtc
    32941 ggcagcccac tgctcgtgcg ccaccaaggg ccggtagcag tcgacatagg tgacgccacg
    33001 gcgggagcac acgtcggcct gcgcctgcgc gagcaccccg atccgggcgt tgagttctgg
    33061 atcgagcgac ggggtcagcc cgacgacgaa cgtcgcgatc cccttggcgg aggcatcgtc
    33121 gaggatgttg gcgagattga gtcgcgagcg cgcagtcgtc aacccttgcc cgaggtcgtt
    33181 ggcgccgacc gccacgacga ggcgccgctc gccgcggccg acccagcgcg gcatacactc
    33241 cgcgcgccac cgcgccatca cgtcaccgga gtcctgcccg cggaccccga ggttgtatgc
    33301 cgtgatctcc acgtcggggt ggtgcgtccg actgacgacg cggccgaccc aacccagggc
    33361 cttggggtcg ccgaacccga cggtgaacga gtcaccgacg aagcacaacc cgatgtcccg
    33421 tcggccatcg tcatcgacgg tgaaggacgc actcggggtg aactcgggtg ccgcctcgcc
    33481 gtccgttgca ctcgggtagg ccgggccgtc gtggccggtc acatggtctt cggtcacgag
    33541 tggttcactc ctcgtcgtcg tcgagtcggg cgaggtaggt cgccagccgc tcgacagggg
    33601 tttcgaactc cgggtgcagg tcgacgaaag tgcgcagatg ctcggccagc cacgagaagg
    33661 tcacttcttc gtcgccccgg cgcgcgtcga gttcctcgat gccacggtcg gtgaagtaca
    33721 ccctcggagg ctatcgcgcc gggtcgcgcg cactcgtcac ggcgcgagca cttgcgcctt
    33781 ccgttaccgg gttactgccc gtcccaacgc cgccgggtca caacgatcgc gagatgagtt
    33841 ccttcatgat ctcgttggtg ccggcgtaga tcttctgcac gcgggcggcg gcatagagcc
    33901 gagcgatcgg gtactccatc atgtagccgt agccgccgaa gacctgcaga cagcggtcga
    33961 tgaccgagca ctgcacgtcg gtggcccagt acttgatccg cgaggcgagc accgcgtcca
    34021 agcgcccagc caggtgctcg gtgatgcagt gatcgaggaa ggtgcgcgcg gccagcgcgt
    34081 cggtggagac ctcggagagc acgaaccggg tgtgctggaa gtcgaggatc ttgcgcccga
    34141 acgcctcgcg ttccttcgag tatgccgtgg cgagacgcac ggcatactcc gccgctgcga
    34201 ccgcgcccac cgcgacaacg aggcgctcct gcgggagttg ctccatgagt tggccgaagc
    34261 cgtgaccctc ctcgccgagg aggttcgccg ccgggacgcg catgtccgag aaggcgagct
    34321 cacgggtgtc ttgcccgtgc tggccgatct tggccaggac ccggccgcgg gtgaagccgg
    34381 gcaggtcgtt gacctcggcg acgagcagcg agatgccttt ggcgcccggc gcgccggtgc
    34441 ggcagacgat gatgacgagg tcggcgtgcg tggcattggt gatgaaggtc ttgctgccgt
    34501 tgacgacgta gtcgtcgcca tcgcggcggg cggtcgtgcg caccgcctgc agatctgagc
    34561 ccgtgcccgg ctcggtcatc gcgatggcgc cgacccactc gccggaggcc agtttgggca
    34621 gccaccgacg cttttgctcc ggcgtgccga actcgacgat gtagtggggg acgatcccgc
    34681 tgtggatgtg caggccgaga gcgtcgtccc cggcccaacc ctgctcctgg aagacgaccg
    34741 cctcgtgcgc gaacgtgccg ccgccacccc cgaactcctc cggcacgctc agcccgagca
    34801 gccctgccgc acccgccttt agccaggttt cgcggtcgac ctggtgctgc tcggcgaacc
    34861 gctcggcgtt cggcgtcacc tcccgcgcga agaacgtccg agccagactg gacaactcgt
    34921 ccagatcgtc ggtcatccac ggtgaacggt agggctgggc gacaacgcgg gccacggttc
    34981 ctcctcgaac gggtctccct taggtctatc gcggcctccg catcgtgtcc accgcacgag
    35041 cgtgacccca cgcacagtcg tcgtcccagc ccacagtggc gtccaccaca caggggtatg
    35101 ccgcgcgctc acttgagcgc gcggcatacc aagggtgtcg aatcgggtca gcgcgcgaag
    35161 gccgccccga gcaacgcagc ctgctcgagc tcgtgcttct tgtgtgaccc agaggccggc
    35221 gaggccgagg ccttgcgggc gatgacgcgc agcgggcggt tctcgccctg ctcggccaac
    35281 agctccggca ggttgagcgc catgaacggc caggcaccct ggttcttcgg ctcgtcctgg
    35341 acccacacga gctcggcgtt gggatatccc cgtagggctg cggcgagctc ctcggccgga
    35401 gccggggcca actgctcgat gcgcagcagg gcggtgctcg tgtcaccgag tttgtcacgc
    35461 tcggcctcga gctcccagac gaccttgccc gacgccatga gcacgcgggt gaccgcgccg
    35521 ccgtcgaggt gacgggtgtc tggcagcacc gggcggaagc cgccggtcgt gaagtcctgc
    35581 ggcatcgacg cggcggcctt gagccgcagc atcgacttgg gggcgaacac gatgagcggg
    35641 cgacgcgggc gggcgtaggc ctgacggcgc aacaggtgga agtgggacgc cggggtcgag
    35701 gggtaggcga tcgtcatgtt gtcctcggcg aacaactgca ggaagcgctc gatgcgggcc
    35761 gacgagtggt ccggtccttg gccttcgtag ccgtggggga gcagcagcac gaccgacgaa
    35821 cgctgcgccc acttctgctc cgaggaggag atgaactcat cgacgatggt ctgcgccccg
    35881 ttggcgaagt cgccgaactg cgcctcccac aggacgagcg catccggccg ctccacggag
    35941 tagccgtact cgaagcccat cgcggcgtac tccgacagca gcgagttgta gacccagaac
    36001 ttcgcctgac cctcctgcag gtggtgcagc ggggtccact cccgtccggt gatcttgtcg
    36061 atgagcaccg cgtgccgctg gacgaaggtg ccgcgctggc tgtcctgacc ggccagccgg
    36121 accggcgtgc cctccatgag cagcgacccg agggcgagca attcgcccat gccccagtcg
    36181 atcccgcctt cgcggaccat cgctgcccga cggtccatga gggccttgag cttggggtgg
    36241 accgtgaatc cgtccggcgg gcggacccag gcgtcgccca gggtgttgag ggtgtcgacc
    36301 gagatggccg tctggcgcca gtcgcgtggg cgcgacacgt cgtcgagcgc ctgcgccgtg
    36361 ggcttctcca ggccgccgtg gccggcgacg tccgaggtgg agtcgacgta tgccgtctgg
    36421 ctggctgccg gtgcggcctg cgcctcgcgg gtctcgagga acacccgctc caactgctgc
    36481 tggtagtcgc gcaacgcggc cgaggcgtcc tcgaccgaga tgtcgccacg accgatgagc
    36541 gactcggtgt agagcttgcg gaccgagcgc ttggcttcga tgaggttgta catgagcggc
    36601 tgggtcatcg acgggtcgtc
//
LOCUS       DAORPW010000008        33375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_181_length_33375_cov_747.268367, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000008 DAORPW010000000
VERSION     DAORPW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 33375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33375
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181_length_33375_cov_747.268367"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PLB86_01560"
     CDS             <1..1221
                     /locus_tag="PLB86_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRC63853.1"
                     /translation="SLAVDHLGTRTDVVAKPLWHDTPEAMATVHGVVKDWRTGEVEPV
                     PGKTLPVLAIAERDYPATFQKMTSIGPLLEKVGMLTKGVPYDVKREIKILRRRNGVAG
                     SGVGAGQPRLKTDVHMADAILHLSATTNGHLSTHGFKNVEKRTGTMLHDLAAEHEGKQ
                     VTFADTQVAPVPVITSPEWSGSESGGRRYSPFTINIERLKPFHTLTGRQQFYLDHDWV
                     IGMGESLPVHRPPLNMTTLFGETPIGGQSEAHGQRSVSVRYLTPHNKWSIHSEYQDNL
                     YMLSLSRGGQTIWMSDADADKVGINDNDWVELVNRNGVVSCRAIVTHRMPEGTIYMPH
                     AQDRLVDVPLTETNQRRGGIHNSLTRIMIKPTHIIGGYAQLAYFFNYIGPTGNNRDEV
                     TMIRRRSSQEVTYE"
     gene            1218..2888
                     /gene="narH"
                     /locus_tag="PLB86_01565"
     CDS             1218..2888
                     /gene="narH"
                     /locus_tag="PLB86_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HRC63854.1"
                     /translation="MKIMAQVAMVMNLDKCIGCHTCSVTCKQAWTNRAGVEYAWFNNV
                     ETRPGLGYPRGYENQDEWQGGWVRTSSGRLKLRSGGRFKKLAAIFSNPKLPSLHDYYE
                     PWTYDYDTVLNAPAQKNFPVARPFSLITGKPMNVSWSANWDDDLGGSRVHAVKDPMLK
                     GIEDKVQFEFEKTFMFYLPRICEHCLNPACAASCPSGAIYKRSEDGIVLVDQDRCRGW
                     RMCISGCPYKKVYFNHRTGKAEKCTFCYPRIEVGLPTVCAETCVGRLRYIGLVFYDAD
                     RVLQAATVEKDTDLYAAQRACFLDPSDPQVQQAARAAGIPQEWVRAAERSPVWKLISR
                     YEVALPLHPEYRTMPMVWYIPPLSPVVDVIRDSGHDAEDADNLFAAIDTLRIPVEYLA
                     NLFTAGDTGPVDRALRRLAAMRSYMRERTMGNEPGPAMAQAVGMSEEDLYEMFRLLAI
                     AKYADRYVIPTAHAEQAHALEELATECALDSDGGVGMGGSGPVGEGSGRLTPVAVENL
                     RVLKARAAAGTSGEELVGGGRKSGRVNLLSWDGKGIPEGLFPPDRKDG"
     gene            2888..3577
                     /gene="narJ"
                     /locus_tag="PLB86_01570"
     CDS             2888..3577
                     /gene="narJ"
                     /locus_tag="PLB86_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HRC63855.1"
                     /translation="MFRRSRHTAAPTRLPDDQLSIAWQVCSLLLDYPSEALLARLELA
                     RRAAGELPAYLAEPVCRVVDHLESTPLGQVQRDFVETFDHTRRGCLYLTYFTTGDTRK
                     RGVALVRLKQAYRRAGVELTSGELPDHLAVVLEFGATADIEAAWRILNDYRASIEVLR
                     ISLQERNSPWADAVVAISRTLPELAGDDAEAIAKLIEQGPPSEDVGLAPYAVDPRLNP
                     LPDYLAVGADR"
     gene            3574..4314
                     /gene="narI"
                     /locus_tag="PLB86_01575"
     CDS             3574..4314
                     /gene="narI"
                     /locus_tag="PLB86_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HRC63856.1"
                     /translation="MTTFLWVIFPYLCLTTFVVGHYWRYRYDKFGWTTRSSQLYEDRL
                     LRIGSPLFHFGMLGVLGGHIIGLVIPQGWTDALGLSRHTYHVIALAAGIPAGIAAVVG
                     LCILVYRRRTTGPVFSATTVNDKVMYAVLGAVIALGIWNTIAGSWLQFGGDYNYRDGV
                     SPYFRSIFGFQPKAALMAAAPLGFQAHALLAFVLFAMWPFTRLVHVFSAPLGYLTRPY
                     IVYRSRDPQSEQGLGSRAPKRGWERVGS"
     gene            4311..4940
                     /locus_tag="PLB86_01580"
     CDS             4311..4940
                     /locus_tag="PLB86_01580"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRC63857.1"
                     /translation="MTGSPDGFAGHPAYDAIILAGGRGSRLGGVDKGAVRISGRTLLD
                     RALDAVGSAARVVVVGDGPVPEGVLSAREEPAFGGPAAAVVAGLHALRMPSRAPRVLV
                     LACDVPGAAEGVGLLMAACAGEEGPSGSAVGRPEVDGWCLAEPDGRLQWLFGLYRVPA
                     LERAAEALGDPSDRSMGRLLGDLHLTAVPAPWRATADIDTPEDLQRWTS"
     gene            4928..5281
                     /locus_tag="PLB86_01585"
     CDS             4928..5281
                     /locus_tag="PLB86_01585"
                     /inference="COORDINATES: protein motif:HMM:NF040429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HRC63858.1"
                     /translation="MDIVRTERTAHDLAAEQAQWQTWIDAVCADLGVPSSLVDVEAIH
                     DLSRQVAHGLARPLAPVSTFILGLALGRALGSSGAAVSGSADGSAESDGSGSSDGRTT
                     AEHRAALSDRIAAHI"
     gene            complement(5567..6682)
                     /gene="ald"
                     /locus_tag="PLB86_01590"
     CDS             complement(5567..6682)
                     /gene="ald"
                     /locus_tag="PLB86_01590"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRC63859.1"
                     /translation="MKVGIPAEVKNHEYRVAITPVGVHELVGHGHEVMIQKGAGLGSS
                     ITDDDYISQGARIIDSADDVWGEADMVLKVKEPVAEEYHRLREGLTLFTYLHLAADKP
                     LTEELMAKKVTGIAYETVQLPSGMLPLLYPMSEVAGCLAPQVGAHAMMRAQGGRGVLM
                     GGVGGVANAKVVVLGAGVAGQNAANIALGMGADVTLLDTDLDKLRMSFWRYDNRVHQI
                     ASSKLSVREQVLGADLVIGTVLIPGAKAPKLVTDDMVAAMKPGSVLVDVAIDQGGCFE
                     GSRPTTHADPTFAVHDAVYYCVANMPGAVPNTSTWALTNATLSYAVKLADKGWKGAIG
                     ADQALALGLNTHDGFVTYKAVADDLDLPYRSLADVLA"
     gene            6868..7356
                     /locus_tag="PLB86_01595"
     CDS             6868..7356
                     /locus_tag="PLB86_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRC63860.1"
                     /translation="MPFEGEPTAYSGLDGLDRTILDALQEDGRLTNVDLARRVGLSPS
                     PCLRRVKSLEERGYIQGYAALLDPALLGRALQVVVMVRLTDQRQQTLAAFESAVEGIE
                     DVLRCSLIAGDADYLLTVQVRDLDSYHALYTKRLGELPGVASLRSLVIMKAVKDTHAL
                     PV"
     gene            complement(7385..7822)
                     /locus_tag="PLB86_01600"
     CDS             complement(7385..7822)
                     /locus_tag="PLB86_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRC63861.1"
                     /translation="MIALDTNLLVYAHREDSPHHVAAAAALQSAITAPGGCAIPWPCL
                     HEFLAVVTHPRIYDPPTPMGTAVAAVRALVDAPSVHLLGEGVSHLDRLAALLASGAVV
                     GPRIHDARVAAICLDHGVDALWSADRDFSWFPKLTVVNPLVAG"
     gene            complement(7819..8070)
                     /locus_tag="PLB86_01605"
     CDS             complement(7819..8070)
                     /locus_tag="PLB86_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63862.1"
                     /translation="MKTTIEIPDALAEAARQVARAGGTTLRDLVVTGLMAEVERRRNA
                     PRVEFTFPTVGGRGLVVDLAPGEVIDRSYGFVSGAGSSR"
     gene            8258..9505
                     /locus_tag="PLB86_01610"
     CDS             8258..9505
                     /locus_tag="PLB86_01610"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRC63863.1"
                     /translation="MTLLAVGVSHHHLGSDHLAELEVLAPDLTRAIESSTAVEGLVVV
                     ATCNRFEAYLDATAFHPAVEAVVAALRATPLADPQAIIEALDVHPGESAVEHLFSVAC
                     GLESMVVGEAQVVGQVRQALAEADAAKRATPALHRLFQDALATAKTVASTTELGGRGR
                     SIASVGLDLLEAQHSPVAGSQALVLGTGSYAGVVVAELTRRGCTDIAVYSRTGRADSF
                     ARTHPVTPVPEPGLLDALARADLVVTCSGASSPTLTDALVKAQRRYAARVLPILDLST
                     GGELGDGVAELDEADVFTLDEIGRSAPAEHVDTLLAAQDVVNRSVAAYLHVEGGRTAA
                     PAITAMRAYVSAIIAREAEVAARRYPPEVAEAIDRSLRRVSGALLHAPSVRAAELART
                     GDLEDYTHAMATLFGIVVEADSW"
     gene            9499..10386
                     /gene="hemC"
                     /locus_tag="PLB86_01615"
     CDS             9499..10386
                     /gene="hemC"
                     /locus_tag="PLB86_01615"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRC63864.1"
                     /translation="MVRLGTRGSLLARTQSTHVAEALYAATGLRAELVIIRSEGDDHR
                     IPLHAPSRPGIFVATLRDALLAGEVDLVVHSFKDLPSAPMPGLVIPAIPARRSPADVL
                     VTATGGGLADLAVGARVGTSSPRRAAALLRVRPDLTIVPIRGNVDTRVAAVAAGTVDA
                     VVLAQAGLERLGHLTDRMTPLPTRVMLPAPAQGALAVECRAADPLADHLAVLDDPRAR
                     LEVTAERAVLTGVEATCTTAIGALARWESGVLTLRAELTEDGTRSHTHAEHSRTLADE
                     HDLDGAAALGAQVAEWLLL"
     gene            10371..11180
                     /locus_tag="PLB86_01620"
     CDS             10371..11180
                     /locus_tag="PLB86_01620"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRC63865.1"
                     /translation="MAALVTATPTGADLLPGPVVLVRPDRNEADAAALRDAGFTVLVE
                     PYLSVTTADDPAPALALLDVLDPRRTGRDTWLVLTSARTMAHWAALVGPHLLYAALDA
                     AHEGGLRVACVGSATAATLPDGMSPTLTATNPDASALLDDLLAATAYPMLALLPGSAI
                     ARPTLADGLTAAGWAVRTAAVYDTRSVLTRPASADLLAAGTAAALVLRSPSAVAAVAG
                     FVQPDPSVTIVAVGPTTTSAVREQGWSAVTPDSPDSGAVARALFRALTRSQ"
     gene            11180..12364
                     /gene="hemE"
                     /locus_tag="PLB86_01625"
     CDS             11180..12364
                     /gene="hemE"
                     /locus_tag="PLB86_01625"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018134455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRC63866.1"
                     /translation="MPTSEQSPVTDERRAVGAESSVLPPTHPLVTGRTSGSILVRAYR
                     GERTATRPIWMMRQAGRSLPEYRAARGDTAMLDACLTPELAAEITCQPVRRHRVDAGI
                     FFSDIVIPVRLAGVGVDIVPGVGPVIDHPIRSAAAVAALPELDPQALAPITEAVARSV
                     ELLGDTPLIGFAGAPFTVASYLVEGRPSRDHAITKALMHEHPDVWDALLAWVARTTGA
                     FLRAQGLAGASALQLFDSWAGTLSVEDYLAHVQPHSAAVLAQVADLGMPRIHFGTRTR
                     HLLVAMRDAGADVMGVDAQTPLDEAVALLGGRTPVQGNIDPALLDGPWAELDAHVRDV
                     LRRGATATGHVVNLGHGVPPTTDPDVLTRIVELVHSVPDLLPDLPPDLPPGPLPHPDG
                     AP"
     gene            12361..>13832
                     /gene="hemG"
                     /locus_tag="PLB86_01630"
     CDS             12361..>13832
                     /gene="hemG"
                     /locus_tag="PLB86_01630"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HRC63867.1"
                     /translation="MTAYAGLRLLVVGGGITGLAAAWEASIQGAEVLLVEASDRFGGK
                     LRTEHVDGLAIEAGPDSFVAYRPAALTLVRELGMGDEVVEVRGGRTVHLRTDGRLQPI
                     PDGMGLVLPTKLGPFVRTPVLRWRHKARALLDLVLPRRLGASDVAIGDLLRARLGEGI
                     VQRFADPMLGGIYGASVDELSLDAVLPILREHERTHRSLIIASLASGRAARAAAQSTS
                     GSGGTTRGPSSPFRSLATGLGALPDRLVDALTERGADLRRHTRVDALTPEGEQTRVRL
                     SDGTEHVVDGVVLAAGAAASADLVEPYAASAAAALRQVRHASTTVVTVAWPESAFATP
                     PTSQGWLEAGDAPVSGVTISSAKWPGRAPAGVVLARAFVPDRVGRLARGSDGEILDAV
                     TRHVSGILGAHQPPTLTRVTRWVGVMPKYTVGHLDRVAAVDAGLAGQPSWTVAGSALR
                     GVGIPDCIADGRAAVSRVLGSPKATANASPTTTATPTTASP"
     assembly_gap    13833..13932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13962..14729
                     /locus_tag="PLB86_01635"
     CDS             13962..14729
                     /locus_tag="PLB86_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HRC63868.1"
                     /translation="MSTQEQPTATETSAPAAAPAAGQAPVTAPATKVTAREINETIRY
                     TCWAVFAAAEGPDAGAAERLAEVVAGLAATDVVVRGFYDVSAMRADADVMVWLHGPTP
                     ESIQTAVRAIRRQGFGPAATFVWSGVGMHRPAEFNKGHVPAFMTGAPAQDYLVVYPFV
                     RSLEWYLLPEAERRAMLVEHGMMGRAWPQVLTNTVAAFALGDYEWMLALEGPALHDIV
                     DLMRHLRAAEARRHVRVEVPFYTGRLVGIAEAVDALR"
     gene            14726..15748
                     /locus_tag="PLB86_01640"
     CDS             14726..15748
                     /locus_tag="PLB86_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRC63869.1"
                     /translation="MSLAYDALVLVGFGGPAGPDDVMPFLRRVTAGRPVPQDRLDEVA
                     ARYLELGGSSPIVAQTEALLAALVERLAARGLEVPAYLGHRNSSPTVPDTLARVAADG
                     HTRVLALASSAFPSYSGCRQYRENLGAGLAAAPGLAVRALGPFPELPGLIDAAADLLA
                     SALRSAGPGATVLVTAHSLPLASAATSGPNGGGYVAALEHAAAAVVARATALAGMPRP
                     PAWRLVFQSRSGSPHVPWLEPDVCDAIREIASDATTQTIVVVPLGFLTDHTEVLWDLD
                     REAAEAAAAAGLGFVRVPTVGTHPAFLDGLADLLAAHLEGRAPAPAQPAFCSDRCCPS
                     PAQMEG"
     gene            15753..16748
                     /gene="hemB"
                     /locus_tag="PLB86_01645"
     CDS             15753..16748
                     /gene="hemB"
                     /locus_tag="PLB86_01645"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRC63870.1"
                     /translation="MRPNASFPSARPRRLRTTPAVRRLVAETRLHPAGLIQPLFVAEG
                     LTEPRPLTLMPGVVQHSVESLRVAVAEAAAAGVGAVMIFGVPEERDATGSGATDPDGI
                     LTVGVRAAVEAGGGIPVIADLCLDEFTDHGHCGVLDTHGRVDNDATLQAYGDMALVLA
                     DAGAHALGASGMMDGQIGAVREVLDAAGHLDTILLAYAAKYASAFYGPFREAVGSTLT
                     GDRRTYQQDPGNAREAIREVRLDLAEGADIVMVKPALAYLDVISRVAAISDVPVAAYL
                     VSGEFAMVEAAAAAGAIDRASAIVEVLTSVTRAGADLVATYWATEVAGWLNGTER"
     gene            16750..>18023
                     /locus_tag="PLB86_01650"
     CDS             16750..>18023
                     /locus_tag="PLB86_01650"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRC63871.1"
                     /translation="MTSSVTSEELFARARAVMPGGVSSPVRAFGAVGGTPRFVASAAG
                     GTITDIEGRTYVDLVGGWGPAIVGHAHPAVVEAVQAAVARSASFGAPCLPELELAEAI
                     CARVAPVERVRFTSSGTEAVMTAIRIARAATGRRVVLKFAGCYHGHADAFLVAAGSGV
                     ATLGLPDSPGVTPAVAADTIVVPYGDLHAVRAAFAAHPGQIAAALTEAIPANMGVVVP
                     PVGFNAALAQVCRADGALLVLDEVMTGFRVGPAGRWGLEGAEQGWAPDLFTFGKVIGG
                     GLPLAAVAGPGALMDLLAPAGPVYQAGTLSGNPAASAAGLATLSLLDDAAYRRLDESA
                     ATVAAMVSAHLAAAGVAHRVNAAGNLFSVFFTADPVTDYESAKRQDTAAYGRFFHELL
                     DRGVWLPPSAYEAWFVSTAHTNRELSVLQAALP"
     assembly_gap    18024..18123
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18391..19713)
                     /locus_tag="PLB86_01655"
     CDS             complement(18391..19713)
                     /locus_tag="PLB86_01655"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRC63872.1"
                     /translation="MSGHPVVDPGPWWEGVAPLLSDLIRLDTQIGGPGERTAAEWVAG
                     RLTDDGLSPVLIESEPGRSTVVARVPGADPTLDPLLIHMHLDVVPAQAANWSVPPLSG
                     EIADGWVWGRGAVDMKNMVATVLTVVRRRLRTGHLPRRPLVLAFFADEENAGVLGAEW
                     AVATHPELFEGCRTAIGEGGGWSVPLAGGQRVYPLHVAEKGWAAMSVTATAPPAHASR
                     VYDDNPLRVLSEALGRLTHDRFPIHLTTQIEGLLRVAAEAAGTTFDPADPEASLHLLG
                     TEAANGAAALRHTAHATRLRAGVQSNVIPGDAWAELDCRYLPGRWDEFLGELHAALGD
                     RVQVRELARTDGLTPMTAGAGPAFFAAATQALRSQDAIASVAPIMLSGGTDATHLQRL
                     GIECFGFNPVLLPEGLDFWSLFHGVDERVPVSSLEFSARVLDDFLDRF"
     gene            complement(19710..20672)
                     /locus_tag="PLB86_01660"
     CDS             complement(19710..20672)
                     /locus_tag="PLB86_01660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HRC63873.1"
                     /translation="MPTAVESDLTLLVRQLRWESEGVVSVVLENADGSALPAWEPGAH
                     VDLHLPGSIVRQYSLCGSPDRSGQWRVGVLREESGRGGSAAVHEKVRPGTVIAARGPR
                     NNFVLRPSAAYVFIAGGIGITPILSMVAAADAAGAQWSLLYGGRTRPSMAFIDELAAY
                     GDHVLIHPQDELGLLPLGEVLRDPRPGTLVYCCGPEPLLRAAEEATAAWPAGSLVVER
                     FVPLPVDPAVSANDTMFTVEAAASGVTVAVPAGTTIVKALEAVGIFPPVSCEEGICGT
                     CETTVLGGIPDHRDSLLSEAEQAEGKTMMICVGRARGDRLVLDL"
     gene            complement(20698..21450)
                     /locus_tag="PLB86_01665"
     CDS             complement(20698..21450)
                     /locus_tag="PLB86_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRC63874.1"
                     /translation="MVIAERVHKKFGLNEVLKGVDLTIDRGEVVVMIGPSGSGKSTFL
                     RCINHLERVDAGRIWVDGQVVGYEQRGHHLHELSERAIARQRAHIGMVFQHFNLFPHM
                     TVLDNLIEAPRRVFGVDREEVTARAEVLLGKVGLRDKIKAYPRHLSGGQQQRVAIARA
                     LCLQPSLMLFDEPTSALDPELVGDVLAVMKDLAASGMTMAVVTHEMGFARDVADRVVF
                     FDSGVIVESGPPDQVILDPQHERTRVFLRAVR"
     gene            complement(21534..22448)
                     /locus_tag="PLB86_01670"
     CDS             complement(21534..22448)
                     /locus_tag="PLB86_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRC63875.1"
                     /translation="MSAVPVLTPAEELTVVPKRHPGRWLAAAVILIIVVRMAISVLGN
                     ERFGWDTVNTYLRDVSIGSGLMMTLGLTVACMVVGVVLGALLAVMRLSANPVVSGAAW
                     AYVAAFRGTPVLVQLLFWYNLAALYPVITLGLPGVALDANQLISPTVAAILGLGLNEA
                     AYMSEIVRAGILSVDHGQQEAASALGLTRMETMRRIILPQAMRVIVPPTGNETIGMLK
                     TTSLVSVMAVHDLLYSAQIISARNYQIIPMLIVASIWYLVVTTILTIGQFYLERRFAR
                     GERTLPETPIQRLRRLLRTHAPSVRGTS"
     gene            complement(22445..23389)
                     /locus_tag="PLB86_01675"
     CDS             complement(22445..23389)
                     /locus_tag="PLB86_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC63876.1"
                     /translation="MRPLMLVTLLATSSLALAACTNAGQASTSTTTAPAAGVAATSTT
                     AGSAAGKAALDPAVAALVPADIKAKGQLSFAMDASYAPFEYFDTDNKTIIGFDADLSQ
                     AIGTTMGIKVNDVNAAFDSILIGLTSQKYDVGMSAFSASAERAKVVDFVTYGAGGSGI
                     AVPKGNPKALSMDPMTLCGRTIAAQKGTIQGLDYLPKFSADCTAAGKAAITADLYPAQ
                     TDANLAVTSGRADAVMADSVSLALQAKATGTLELAAGPDYNPTNVAVAVPNDSPLAAP
                     IGAALKALDKNGTLAAIMAKWGLPTQFATQAKIGEIIK"
     gene            complement(23425..23841)
                     /locus_tag="PLB86_01680"
     CDS             complement(23425..23841)
                     /locus_tag="PLB86_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63877.1"
                     /translation="MTPAADGSVTLAACPDTADRGEDWRWRIWRGPPAAGSGGEVFVE
                     TDPDSVRATTSFVDAGPVTVSRRAGEEVVVVPLVGHHLVADSAGPWQRWLHPGDAFVL
                     EGEESETVNLSSAPGPARVAIVRLNTLNDAPLRWVP"
     gene            complement(23872..24987)
                     /locus_tag="PLB86_01685"
     CDS             complement(23872..24987)
                     /locus_tag="PLB86_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRC63878.1"
                     /translation="MPHVLNAWYVAAWSHEVTRDPVARTICQQPVVLYRAKDGSAVAL
                     ADRCAHRGYPLSAGRLVEDSIECGYHGFAFGCDGVCTRVPAQSTIPSRSKVRAYPLHE
                     KDGWVWIWTGEAERADVTTIPDTHWMNDPAWATVTHSYLFNCRADLVHDNLLDLTHES
                     FIHRTTVGDDYIYEHGITVEVDGTTVTVDRLMPGVEAPPLYASTMGVEGLFDRFHATE
                     FAVPSHHTLHSGITGRGRPREEGYLIKVLNGITPIDEHTCWYYYAFCRNFGIENTAAT
                     EDLRIGLGTVLDEDAEALRLQELALQTRPKGEHDVLIAQDAGVAKARRVMSQLLAAES
                     VAAAGPASGTSEARYAASPAAAAAAAPPAASATASVG"
     gene            25257..25892
                     /locus_tag="PLB86_01690"
     CDS             25257..25892
                     /locus_tag="PLB86_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRC63879.1"
                     /translation="MGAVVYEAIRDAIVEGRIAADEQLVQEQLAAELGVSRTPVRDAL
                     NRLSHEGLVRAVLGRGYQVIDLTDQDINDVFVVRERLEMLAVEQSAGRLTALHQARAR
                     TLIEEMRATDPADAVAQYELNRRFHQTIIEPCNNAILLGLLDQLWDLPVSRRITKSYL
                     RDAANVAHMVDEHDAILAAAAEGDLPRLLDLSRAHLTLGYGEARADSQPPT"
     assembly_gap    26054..26153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26154..26883
                     /locus_tag="PLB86_01695"
     CDS             <26154..26883
                     /locus_tag="PLB86_01695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC63880.1"
                     /translation="HGHGHGHGHGHGHDQDHGHGHEPDAATFFDEVAATWDDDPKRAE
                     RATAIADAIRASVPLTRQMSGFEFGSGTALLSRSLAPDLGPITVSDPSRGMLEVVAQR
                     LAAEGDTQLTPLLLELGVDPIPGGAAYDLAYGMLALHHVDDVPAALRSLRAALRPGGY
                     LCIADLDADGGAYHAGHSHFHGHHGFDREALAAQLIEGGFVDPHLRTVYHLRKPVDGV
                     EQDFPLFLAVAQVGQVGQAGPDRA"
     gene            27110..27472
                     /locus_tag="PLB86_01700"
     CDS             27110..27472
                     /locus_tag="PLB86_01700"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HRC63881.1"
                     /translation="MNAVIVVSTAVIVALTAAALLQFALTKSGSIDRNAMIGIKTSAT
                     LASDSAWKAGHVAAQPALMTAAVTGAAALVTVLVAWLLAPQAELALAVVSATGLVVML
                     GLFVRVAFSANHAARNAV"
     gene            complement(27492..28697)
                     /locus_tag="PLB86_01705"
     CDS             complement(27492..28697)
                     /locus_tag="PLB86_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63882.1"
                     /translation="MLSAYVPLFRVPGALRFIAGTALSRVGGSMFGVAIIVMLSERRG
                     SFGLAGAISAVGVGTLAVAGPFIGRLIDKHGQRRIALPFIVSSAIFGTSAAVLSIVNA
                     PVWTIFVAYALSAVLPEPGPMSRARWAHIYRDDADQLHTAMSFEQVADEGSFVIGPVV
                     AVLVSTLWFPEAGLLIAELLFTVGMVAFLTATATEPPVVAHENRPAGLAIRWPGLMLV
                     ATALTMTGVIFGSNEVIAVAFADARGNTGFSSVVLGGFALGSTLAGIVFGSRKFNSTL
                     SRRLLLAAAGMFVLEAPAFFMSDLWPLAVVMFVAGSATAPMLITSLSLAQVLVPKAMV
                     TEGMAVAITGILIGISGGAAVGGWAIEAWGAQRAYAVPILAGAAALAIIVARFRHVQR
                     AELTASAEA"
     gene            complement(28900..29625)
                     /locus_tag="PLB86_01710"
     CDS             complement(28900..29625)
                     /locus_tag="PLB86_01710"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HRC63883.1"
                     /translation="MTPELAAALVGLALVDATSFGTLVLPTLMLVQPRVRASRVVLYL
                     AAISAFYFVLGVALLFAAERVLALVAATERSRVVDMVQLVVGLALLAGSFWPDLPWVK
                     QRRQERDAAGGGRRPGWRDRVLGPEARSRTVIAVAVGAGLVEAASMLPYLGAVGMLTS
                     SVASTPERLAALASYVVVMCLPALILLAARLLLGARVDGALGRLARWLDRQSRNAIWW
                     VVGVVGFLIARDAASRLWPGSMG"
     gene            29683..30309
                     /locus_tag="PLB86_01715"
     CDS             29683..30309
                     /locus_tag="PLB86_01715"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRC63884.1"
                     /translation="MPKLVDHDERRHEIARALWSVVARGGFEAASARTVAAEGNWSLG
                     ALRHYFSSQDELVEFAARELIDGAAARIATVLETMPAGLPRCRMALEQLLPLDDVRRG
                     EVRAWLAILVKAEVSPSLAELREVALDGERQLCRLVVAELSGHPYPTSPSDWPAALEY
                     AAVDLHAWIDGHCLQAASSHRLLPADAARAALDRYLRQLSVTVAPGTA"
     gene            complement(30376..30654)
                     /locus_tag="PLB86_01720"
     CDS             complement(30376..30654)
                     /locus_tag="PLB86_01720"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HRC63885.1"
                     /translation="MSTKTDRLNLRCSSATLATLREAAEVQDQDLTSFILGAALDRAR
                     TVLAEDRVLRLDPHEVLQLERALDAEPAAIPQLAALLRSVRASASARV"
     gene            complement(30725..31258)
                     /locus_tag="PLB86_01725"
     CDS             complement(30725..31258)
                     /locus_tag="PLB86_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC63886.1"
                     /translation="MIALEVPIVAFGKSFDRSRFTSGNADLDDWLRTKAGQGERSDNT
                     RTFLALDPGGERVIGYYATTTYRLELDEAARALGAGKRRYPVPAVLLARLAVDGEWAG
                     RGVGRTLLVHALRQIAEASRSVGFEVVVVHAADESAVTFYARYGFTAFVSHPRHLLMP
                     TKQLRATFEAERLGHPR"
     gene            31745..32281
                     /locus_tag="PLB86_01730"
     CDS             31745..32281
                     /locus_tag="PLB86_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63887.1"
                     /translation="MALPTTTITVTQTALSTTTETVTRTVTPTIGSNTGTGVAATGNS
                     GRTYLVDLQPVDSSLEQRVVDFSDVTYPNSLINLMSGCSQTGPVDYLVPGGATQLVAE
                     VGVTRDSLEPDSRVTFQVTVDGQRAEPITAQVAQHATLRVVVSSKSRVRLETSIDKNR
                     RTNCNTEAVAVWGDPYFS"
     gene            32518..33144
                     /locus_tag="PLB86_01735"
     CDS             32518..33144
                     /locus_tag="PLB86_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC63888.1"
                     /translation="MTRYRPEHKEATRRRLIEAAGRRFKSDGIDGSGIATLVADAGLT
                     NGAFYNHFASKDDLIATVVAAQLTDQAAVIDALPVGPASMQTYLRQYLSPAHRDDRAG
                     GCPSAALLDEIGRCDEATRAAYTVGVEPMIAAVARHLDDGDPQGAQERAIGLLTLLVG
                     SLQLARAVTNPALSQQILATAYTHALSLAGVQPGASTLPVAPPTQESS"
     gene            33141..>33375
                     /locus_tag="PLB86_01740"
     CDS             33141..>33375
                     /locus_tag="PLB86_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRC63889.1"
                     /translation="MKAFVVTHYGPDGLAAAEVATPTAGPGDVLVDIRAASINPLDKM
                     IRNGEFKHLIKYKPPFALGHDLSGVITAVGSDVR"
BASE COUNT         5089 a        11825 c        11130 g         5031 t
ORIGIN      
        1 tcgctcgcgg tcgaccacct cggcacccgc accgacgtcg tcgccaagcc cctgtggcac
       61 gacacgcccg aggcgatggc gaccgttcac ggagtcgtca aggactggcg caccggagag
      121 gtggagccgg tgccgggcaa gacgctgccg gtcctcgcga tcgccgaacg ggactacccg
      181 gcgaccttcc agaagatgac ctccatcggc ccgctgctgg agaaggtcgg catgctcacc
      241 aagggcgtgc cctacgacgt caaacgcgag atcaagatcc tgcggcgccg caatggggtc
      301 gccggctcgg gggtcggcgc cggtcagcca cgcctcaaga ccgacgtgca catggccgat
      361 gccatcctgc acctgtcggc gacgacgaac gggcacctgt cgacgcacgg cttcaagaac
      421 gtcgagaagc gcactggcac aatgctgcac gacctggccg ccgagcacga gggcaagcag
      481 gtgaccttcg ccgacaccca ggtcgcgccg gtccctgtca tcacctcacc ggaatggtct
      541 ggctccgagt ccggcggacg tcgctacagc ccgttcacca tcaatatcga acggctcaag
      601 cccttccaca ccctcaccgg gcgacaacag ttctacctcg accacgactg ggtcatcggc
      661 atgggcgagt cgctgccggt gcaccgcccg ccgctcaaca tgacgaccct gttcggcgag
      721 acccccatcg gcgggcagtc cgaggcgcac gggcaacgca gcgtctcagt ccgttacctg
      781 acgccgcaca acaagtggtc gatccacagc gagtaccagg acaacctcta catgctctcg
      841 ctctcccgcg gcgggcagac gatctggatg tccgacgccg acgccgacaa ggtcggcatc
      901 aacgacaacg actgggtcga gttggtcaac cgcaacgggg tcgtctcgtg ccgtgccatc
      961 gtcacccacc ggatgcccga aggcacgatc tacatgccgc acgcgcagga ccggttggtc
     1021 gacgtgcctc tcaccgagac caaccagagg cgaggcggca tacacaactc gctgacccga
     1081 atcatgatca agccgacgca catcatcggt ggctacgccc agttggccta cttcttcaac
     1141 tacatcggcc ccaccgggaa caaccgcgac gaagtgacga tgatccgccg tcgctcctcg
     1201 caggaggtga cctacgaatg aagatcatgg ctcaggtagc gatggtcatg aacctcgaca
     1261 agtgcatcgg ctgccacacc tgctcggtga cctgcaagca ggcgtggacc aaccgcgcgg
     1321 gcgtggaata tgcctggttc aacaacgtcg agacccggcc gggccttggc tatccccgcg
     1381 gctacgagaa ccaggacgag tggcagggcg gttgggtacg cacctcgtca ggtcggctga
     1441 agttgcgctc cggcggtcgg ttcaagaagc tcgccgcgat cttctccaac cccaaactgc
     1501 cgtcgctgca cgactactac gagccgtgga cctacgacta cgacacggtg ctcaacgccc
     1561 cggcccagaa gaacttccct gtcgcacggc ccttctcgct catcacgggc aagccgatga
     1621 acgtctcgtg gtcggccaac tgggacgacg acctcggcgg ctcgcgcgtg cacgccgtca
     1681 aggaccccat gctcaagggc atcgaggaca aggtccagtt cgagttcgag aagacgttca
     1741 tgttctacct cccgcggatc tgtgagcact gcctcaaccc cgcgtgcgct gcgtcgtgcc
     1801 cgtccggtgc gatctacaag cgctccgagg acggcatcgt gctcgtcgac caggaccgct
     1861 gccgcggctg gcggatgtgc atctcgggct gcccctacaa gaaggtctac ttcaaccacc
     1921 ggaccggcaa ggccgagaag tgcaccttct gttacccgcg tatcgaggtc ggtctgccga
     1981 cggtgtgcgc cgagacctgc gtcggtcggc tgcgctacat cggcctggtg ttctacgacg
     2041 ccgaccgggt cctgcaagcg gccacggtgg agaaggacac cgacctgtat gccgcgcagc
     2101 gcgcctgctt cctcgacccc agcgacccgc aggtccagca ggcggcccgc gccgccggga
     2161 tcccgcagga gtgggtccgg gcggccgaac gctcgcccgt ctggaagttg atcagccgct
     2221 acgaagtggc cctgccgctg cacccggaat accgcacgat gccgatggtc tggtacatcc
     2281 cgccgctctc cccggtggtt gatgtcatca gggactccgg gcacgacgcc gaggatgccg
     2341 acaacctctt cgcggcgatc gacaccctgc ggatccccgt tgaatacctc gccaacctct
     2401 tcaccgccgg ggacaccggt ccggtcgaca gggcactgcg tcgcctcgcg gccatgcggt
     2461 cctacatgcg cgagcggacc atgggcaacg agcccggccc agcgatggcc caggccgtgg
     2521 ggatgtcgga ggaagacctc tacgagatgt tccgactgct ggcgatcgcg aagtacgccg
     2581 accgctatgt catcccgacc gctcacgccg aacaggccca cgccctcgag gaattggcca
     2641 ccgagtgcgc gctcgacagc gacggtggcg tgggaatggg tggcagcggg ccggtcggcg
     2701 aaggttccgg ccgcctcacc cctgtggccg tcgagaacct ccgagtgctc aaggcccgcg
     2761 cggccgccgg cacctccgga gaagagttgg tcggcggcgg gcgcaagagc ggccgggtca
     2821 acctgctctc gtgggacggc aagggcatcc ctgaaggcct cttcccaccc gaccggaagg
     2881 acggctgatg ttccggcgct cacggcatac ggccgctccc acccgactgc ccgacgacca
     2941 gttgagcatc gcgtggcagg tctgctcgtt gttgctcgac tacccgagcg aggcgctcct
     3001 cgcgcggttg gaactcgcgc ggcgagcggc cggcgagttg ccggcatacc tcgcggagcc
     3061 ggtatgccgc gtggtggacc acctggaaag cacgcccttg gggcaggtgc agcgtgactt
     3121 cgtggagacc ttcgaccaca cccgtcgcgg ctgcctctac ctcacctact tcaccaccgg
     3181 cgacacccgc aaacgtgggg tggccctcgt gcggctcaag caggcctatc gccgggcggg
     3241 ggtcgagttg accagtggcg agttgcccga ccacctggcg gtcgtgctcg agttcggcgc
     3301 gacggccgac atcgaggcgg cctggcggat cctcaacgac tatcgcgcga gcatcgaggt
     3361 gctgcggatc tcgttgcagg agaggaactc tccttgggct gacgccgtcg tcgccatcag
     3421 ccggacgctc cccgaactcg ccggtgacga cgccgaggcg atcgccaaac tcatcgagca
     3481 agggcccccg tcggaggacg tcggcctggc gccctacgcc gtggacccgc ggctcaatcc
     3541 cctgcccgac tatctcgccg ttggagccga ccgatgacga ccttcctgtg ggtcatcttc
     3601 ccctacctgt gtctcacgac cttcgtcgtc gggcactact ggcgctaccg ctacgacaag
     3661 ttcggctgga ccacccgctc cagccagctc tatgaagacc gactgctgcg gatcggctcg
     3721 ccgctcttcc atttcgggat gttgggggtc ctcggcggcc acatcatcgg tctggtcatc
     3781 cctcaggggt ggaccgatgc gctcgggctg agccggcata cctatcacgt catcgcgctg
     3841 gccgcgggca tccccgcggg catcgccgcg gtggtcgggc tgtgcatcct cgtctaccgc
     3901 cgacggacga ccggtccggt cttcagcgcg acgacggtca acgacaaggt catgtatgcc
     3961 gtcctcggcg cggtcatcgc gttgggcatc tggaacacca tcgccgggtc gtggctgcag
     4021 ttcggcggcg actacaacta ccgcgacggg gtgtcgccct acttccgcag catctttggc
     4081 ttccagccca aggctgcgct catggcggcg gcccccttgg gcttccaggc ccacgcgctg
     4141 ctcgcgttcg tgctcttcgc gatgtggccg ttcacccggc tcgtccacgt cttctccgcg
     4201 ccgctgggct acctcacccg tccctacatc gtctatcgct cacgtgatcc gcagtctgag
     4261 caaggcctgg gcagccgggc accgaagcgg ggttgggaac gagtgggctc gtgaccggta
     4321 gccctgacgg gttcgccggc cacccggcat acgacgcgat catcctcgcg ggcggacgtg
     4381 gctcgcggct cggcggggtc gacaaggggg ccgtgcgcat ctctgggcgg accctgctcg
     4441 accgcgcact cgacgcggtc ggctcggccg cgcgcgtcgt cgtggtcggc gatggtcccg
     4501 tccccgaggg tgtgttgagt gcgcgcgaag agcctgcctt cggggggccg gccgccgccg
     4561 tggtcgccgg gctgcacgcc ttgcgtatgc cgtcccgcgc acctcgcgtg ctcgtcctgg
     4621 catgcgacgt gcctggcgca gcagagggcg ttgggctgtt gatggctgcg tgtgccggcg
     4681 aggagggtcc gtcggggagt gcggttggtc ggcccgaggt cgacggctgg tgcctggccg
     4741 agcccgatgg ccggctgcag tggctcttcg ggctctatcg cgtgcctgca ttggagcgag
     4801 cggccgaggc gctcggcgac ccgagcgacc ggtcgatggg tcggctgctc ggcgacttgc
     4861 acttgaccgc tgttcctgcc ccgtggcggg ccaccgccga catcgacacc ccggaggact
     4921 tgcaacgatg gacatcgtga ggaccgaacg cacggcacat gatctggccg ccgagcaggc
     4981 acagtggcag acctggatcg acgccgtgtg tgccgacctg ggggtcccct cgtcactcgt
     5041 ggacgtcgag gcgatccacg acctgtctcg acaggtcgcc cacgggctcg ctcgaccgct
     5101 ggcgcccgtg agcaccttca tcctcggcct ggcgctcggt cgagcactgg gctcgtccgg
     5161 ggctgccgtg tcagggtcgg ccgatgggtc cgctgagtcc gatgggtcag ggtcgtccga
     5221 tgggcgaacg accgctgagc accgggcagc actctcggat cggatcgccg cacacatctg
     5281 accccagaaa caccgagcga cctacctgcc tgtcacaaac caccggcggg tgtgccgaaa
     5341 aacggggcca cccggcatac ccctcgccca gatcaccggc gggtgtgggg atatcggggc
     5401 aatcaagggc cgctagccaa caagacgtcg gcgggtgtga ggatcttggg accagcgtgg
     5461 tcctgaggcg gaggaacagc gagccccggg gcctgacgga tcaggctccc ggggctcggt
     5521 cgtccacacg gttcggattg gggtgggaga gcaccccccg tccgaatcag gccaggacgt
     5581 ctgccaagga gcggtaaggc aggtcgaggt cgtcggccac ggccttgtag gtgacgaaac
     5641 cgtcgtgagt gttcaggccg agggccaggg cctggtcggc gccgatggca cccttccaac
     5701 ccttgtcggc gagcttcacg gcatacgaga gggtcgcgtt ggtgagcgcc caggtggagg
     5761 tgttcgggac ggccccaggc atgttcgcga cgcagtagta gacggcgtcg tggaccgcga
     5821 aggtcgggtc ggcgtgcgtc gtcgggcggc tgccctcgaa gcatccaccc tggtcgatgg
     5881 cgacgtcgac gaggaccgag ccgggcttca tcgcggcaac catgtcgtcg gtgacgagtt
     5941 tgggggcctt ggcgccgggg atgaggacgg tgccgatgac gaggtcggcg ccgagcacct
     6001 gctcacgcac ggagagcttc gaggaggcga tctggtggac gcggttgtcg tagcgccaga
     6061 agctcatccg caacttgtcg aggtcggtgt cgagcagcgt gacatcggct cccatgccga
     6121 gggcgatgtt ggccgcgttc tgaccggcga cgcccgcgcc gaggacgacg accttggcgt
     6181 tggcgacgcc gccgacgcca cccatgagga caccgcggcc accctgggcc cgcatcatcg
     6241 cgtgcgcgcc gacctggggg gccaggcagc cggcgacctc agacatcggg tagagcaggg
     6301 ggagcatccc ggagggcaac tggaccgtct cgtaggcgat gccggtgacc ttcttggcca
     6361 tcagctcctc ggtgagcggc ttgtcggcgg ccaggtgcag gtaggtgaag agggtcagtc
     6421 cctcgcgcag ccggtggtac tcctcggcga cgggctcctt gaccttgagg accatgtcgg
     6481 cctcgcccca cacgtcgtcg gcggagtcga tgatccgtgc gccctggctg atgtagtcgt
     6541 cgtcagtgat cgaggagccg agcccggcgc ccttctggat catgacctcg tgcccgtggc
     6601 cgacgagttc gtggacgccg accggggtga tggcgacccg gtattcgtgg ttcttgacct
     6661 cggcggggat cccaaccttc atgtcgtcac catttctcga ccccgcgggg ccgctgtcct
     6721 gtccgaggcc ccgcagggcc ggccgcgtcg tcgcgggctc gtcggcaatt ctagacgcgg
     6781 ggctctggca tgtggtgccc ttctacaccc cgctctgccc tattcttggc atgttctgct
     6841 tctcataccg acctctagga gtgactcgtg cccttcgaag gtgagcccac ggcatactcg
     6901 ggcttggacg gactggaccg aacgatcctc gacgcgctgc aagaggacgg ccgactcacc
     6961 aacgttgacc tcgcgcgtcg ggtggggttg tccccctcgc cctgcctgcg gcgggtcaag
     7021 tcactggagg agcgcggcta catccagggg tatgccgcgc tgctggaccc cgcgctgctc
     7081 ggccgggcgc tgcaggtcgt cgtcatggtg cgccttaccg atcaacggca gcagaccctg
     7141 gcggcgttcg agtcggccgt cgagggcatc gaggacgtcc tgcgctgctc cctcatcgcc
     7201 ggcgacgccg actacctgct gaccgtgcag gtgcgcgacc tcgactcgta tcacgcgctc
     7261 tacaccaagc ggctcggcga actccccgga gtggcgtcgc tgcgcagcct cgtcatcatg
     7321 aaggcggtca aggacaccca cgccctcccg gtgtgagggt cgccgccctc gcactccggc
     7381 acactcagcc ggccaccaag ggattcacca ccgtgagctt cgggaaccag gagaaatcac
     7441 ggtcggctga ccacagggca tcgacgccat ggtcaaggca gatcgcggca acccgggcgt
     7501 cgtggatgcg cggaccgacg accgcgcccg aagccaacag ggcggcaagt cggtcgaggt
     7561 gactcactcc ctcgcccagc agatgaaccg acggagcatc gaccagggcc cggacggctg
     7621 ccactgcggt gcccattggc gtcggagggt catagatccg cgggtgggtg acgacagcga
     7681 ggaactcgtg cagacacggc caggggatcg cacacccgcc gggggcggtg atcgccgact
     7741 ggagtgccgc ggccgctgcc acgtggtgcg gcgagtcctc gcggtgggcg tagaccagca
     7801 gattcgtgtc gagagcgatc acctcgacga gcccgcccct gagacgaatc cgtaggaccg
     7861 atcgatgacc tcgccgggtg cgaggtcgac caccaggcca cgccccccga cggtggggaa
     7921 ggtgaactcg acccgtggcg cgttcctgcg tcgctcgacc tccgccatca ggccggtgac
     7981 gaccaagtcc cgcagcgtcg tccccccagc ccgcgccact tgcctcgcag cttcggcgag
     8041 agcatccggg atctcgatag tcgtcttcat acggcttacc gtacttcatc acccgtattc
     8101 ccgtactcgt ggtgccgccc cggcgagccg ccggaggacc accattcccg cctcggccca
     8161 cacccggttg tccaagccgg ataacgcctt cccaccagcc gtttttcgac actggcggtg
     8221 gtaaaagctc acccctcgcg tggttaccat cgaatccgtg accttgctcg ccgtgggtgt
     8281 cagccaccac cacctcggct cggaccatct cgccgagctc gaggtgctgg cgcccgatct
     8341 cacccgagcc atcgagtctt ccactgcggt cgagggcctc gtcgtggtcg ccacgtgcaa
     8401 tcggttcgag gcgtatctcg atgccaccgc gttccatccg gccgtcgagg ccgtggtcgc
     8461 cgccctgcgc gcgaccccac tggccgaccc gcaggcgatc atcgaagccc tggacgtcca
     8521 ccccggcgag agcgccgtcg agcacctctt ctcggtcgct tgcggcctgg agtcgatggt
     8581 cgtcggcgag gcccaggtcg tcggccaggt ccgccaggcg cttgccgagg ccgatgccgc
     8641 caagcgcgcc accccggcgc tgcaccggct gttccaggat gccctcgcaa ccgccaagac
     8701 ggtggcctcg acgaccgagc tcggcggtcg cggccgctcg atcgcctccg tcggcctcga
     8761 cctgctcgag gcccagcact cccccgtcgc tgggtcgcag gcgctcgttc tgggcacggg
     8821 cagctacgcc ggcgtcgtcg tcgccgagct cacccggcgc ggctgcaccg acatcgcggt
     8881 ctactcccgg accggccgcg ccgactcctt cgcccgcact caccccgtca cgccggtccc
     8941 cgagcccggc ctcctcgacg ccctcgcccg ggccgacctc gtcgtcacct gcagtggcgc
     9001 cagcagcccg acgctcaccg atgccctcgt caaggcccag cgcaggtatg ccgcgcgggt
     9061 cctgccgatc ctcgacctgt ccacgggcgg ggagctcggg gacggcgtgg ccgagctcga
     9121 tgaggccgac gtgttcaccc tcgacgagat cggccgcagc gccccagccg agcacgtcga
     9181 cacgcttctg gcggcgcagg acgtcgtcaa ccggtcggtc gcggcatacc tgcacgtcga
     9241 aggcggtcgc acggcggcac cggcgatcac cgccatgcgt gcctacgtga gcgcgatcat
     9301 cgcccgcgag gccgaggtcg cggcccgccg ctatcccccc gaggtcgccg aggccatcga
     9361 tcgctccctg cgccgggtga gcggcgcgct gctgcacgcc ccgtcggttc gcgccgcgga
     9421 gctcgcccgc acaggtgacc tcgaggacta cacacacgcg atggcaacgt tgttcggcat
     9481 cgtcgtcgag gccgactcat ggtgaggctg gggacgcgcg gcagcctcct ggcccgcact
     9541 cagtccaccc acgtcgcgga agccctctat gccgcgaccg gcctgcgcgc cgaactcgtc
     9601 atcatccgct cagaggggga cgaccaccgg atcccgctgc acgctcccag ccggccgggg
     9661 atcttcgtcg ccacgttgcg cgacgccctg ctggcgggcg aggtcgacct cgtcgttcac
     9721 tcgttcaagg acctgcccag cgccccgatg cccggcctgg tcattcccgc gatccccgcg
     9781 cgccgatccc cggccgacgt gctcgtgacg gcgaccggag gtggtctcgc cgacctcgcc
     9841 gtgggtgcca gggtcgggac gagcagcccg cgacgcgctg ccgcgctgct gcgggtccgc
     9901 cccgacctca cgatcgtgcc gatccgcggc aacgtcgaca cccgggtcgc ggcggtggct
     9961 gccgggaccg tggacgccgt cgtcctggcc caggccgggc tcgaacggct cggccacctc
    10021 accgaccgga tgaccccgct gcccacccgg gtgatgcttc ccgcccccgc ccaaggcgcc
    10081 ctcgccgtgg aatgccgggc ggccgatccc ctcgccgacc acctcgcagt gctcgacgac
    10141 ccgcgcgccc ggcttgaagt gaccgccgaa cgcgccgtcc tcaccggcgt cgaggccacc
    10201 tgcacgaccg ccatcggcgc cctggcgcgt tgggagagtg gggtgctcac gctgagggcc
    10261 gaactcaccg aggacggcac ccgcagccat acccacgcgg aacacagccg gaccctcgcc
    10321 gatgagcacg atctcgacgg cgccgccgcc ctgggtgccc aggtggcgga gtggctgctc
    10381 ttgtgacggc caccccgacc ggcgcggacc tcctccctgg ccccgtcgtt ctcgtgcggc
    10441 cggaccgcaa cgaggcggat gccgctgcgc tgcgtgacgc cgggttcact gtgctcgtcg
    10501 agccctatct gagcgtgacc acggccgacg acccggcacc ggccctcgcc ctcctcgacg
    10561 tgctcgaccc gcgccgcacc ggccgcgaca cctggctcgt gctcacctct gcacgcacca
    10621 tggcgcactg ggcagcgctg gtcggccctc atctcctgta tgccgcgctc gacgccgccc
    10681 acgagggcgg cctgcgggtg gcctgcgtcg gatctgctac tgcagcaaca cttcccgacg
    10741 ggatgagccc caccctcacg gccaccaacc cggacgcctc ggctctgctc gacgacctcc
    10801 tcgccgcaac ggcatacccg atgctggcgc tgctgcccgg aagtgccatc gctcgcccga
    10861 cccttgcgga cggcctcacc gcagcgggct gggcggtccg cacggcggcg gtctatgaca
    10921 cgcgatcggt gctgacccgg cccgcgagcg ccgacctgct cgccgccggc accgccgccg
    10981 cgctcgtcct gcgttcgccg agtgcggtcg ccgccgtcgc ggggttcgtc cagcctgacc
    11041 cctccgtcac catcgtcgcc gtcggcccga ccacgacctc ggccgtgcgt gagcaggggt
    11101 ggtcggcggt cacccccgac agcccagact cgggcgccgt tgctcgcgcc ctcttccgcg
    11161 ccctcaccag gagccagtga tgccgacgtc cgagcagtcc cccgtcaccg acgagcgacg
    11221 ggcggtcggt gccgagtcgt cggtgttgcc tccgacgcac ccgctcgtca cgggccgcac
    11281 gtcgggctcg atcctcgtgc gcgcctatcg cggtgaacgc accgcgactc ggccgatctg
    11341 gatgatgcgc caggccggtc gctcgctgcc cgagtaccgc gcggcccgcg gcgacacggc
    11401 gatgctcgat gcctgtctca cgcccgagct ggccgccgag atcacctgcc agccggtgcg
    11461 tcgccatcgc gtcgacgcgg gaatcttctt ctccgacatc gtgattccgg tgcgtttggc
    11521 tggcgtgggc gtcgacatcg tccccggcgt gggcccggtc atcgaccacc cgatccgctc
    11581 ggccgccgcg gtcgccgccc tgccggagct cgacccccag gccctcgcgc ccatcaccga
    11641 ggctgtcgct cggtcggttg aactgctcgg cgacacccct ctcatcgggt tcgcgggtgc
    11701 gccgttcacc gtcgcgtcct atctcgtcga ggggcgaccc agcagggacc acgccatcac
    11761 caaagccctc atgcacgagc accccgacgt gtgggatgcg ctgttggcct gggtcgctcg
    11821 caccaccgga gccttccttc gcgcgcaggg cctcgccggg gccagcgccc tgcaactgtt
    11881 cgattcgtgg gccggcacgc tgagcgtcga ggactacctc gcccacgtgc aaccgcactc
    11941 cgccgccgtc ctcgcccagg tcgccgacct cggtatgccg cgcatccact tcggcacgcg
    12001 cacccgccac ctgctcgtgg ccatgcgtga tgccggggca gatgtcatgg gggtcgacgc
    12061 gcagactccg ctcgatgagg ccgtcgcact gctcggtggg cgcactcccg tccagggcaa
    12121 catcgacccc gcgctcctcg acgggccgtg ggccgagctc gacgcccacg tccgtgacgt
    12181 cctgcgccgg ggcgcgaccg caacgggcca tgtcgtcaac ctcggccacg gcgtcccgcc
    12241 gacgacggac cccgacgtcc tcacgcggat cgtcgagttg gtccactccg tgccagacct
    12301 cctgcccgac ctcccgcccg acctcccgcc cggcccgctg ccccaccccg acggtgcgcc
    12361 gtgacggcat acgccgggct gcggttgctc gtcgtgggcg gcgggatcac cgggctggcg
    12421 gcagcctggg aggcgagcat ccagggcgcg gaggtcctgc ttgtcgaggc gagcgaccgc
    12481 ttcggcggca aactgcggac ggagcacgtc gacggcttgg ccatcgaggc cgggccggac
    12541 tcgttcgtcg cctatcggcc cgccgctctg accctcgtgc gcgagctcgg gatgggcgac
    12601 gaggtcgtcg aggtccgtgg cggccgcacg gtgcacctgc gcaccgacgg acgcctgcag
    12661 ccgatccctg acgggatggg cctcgtgctg ccgaccaaac tcggcccttt cgtccgcacc
    12721 ccggtcctgc gctggcggca caaggcccgc gcactgctcg acctggtgct gccgcgacgc
    12781 ctcggcgcga gcgacgtcgc gatcggcgat ctgctgcgcg cgcggcttgg cgagggcatc
    12841 gtgcaacggt tcgccgaccc gatgctcggc ggcatctacg gggcgagcgt cgatgagctc
    12901 agcctcgatg ccgtcctgcc gatcctgcgt gagcacgagc gcacccaccg cagcctcatc
    12961 atcgccagcc tcgcctccgg tcgcgccgcg cgggcggccg ctcagagcac cagcggcagt
    13021 ggagggacga cccgcggacc gtcctcgcct ttccgcagcc tcgccaccgg gctcggcgcg
    13081 ctacccgacc gactcgtcga cgccctcact gagcgcggcg ccgacctgcg gcggcatacc
    13141 cgcgtcgacg ccctcacccc cgagggtgag cagacccggg tgcggctctc cgacggcacc
    13201 gagcacgtcg tcgacggggt cgtcctcgcc gccggcgccg ccgcctcggc cgacctggtg
    13261 gagccgtatg ccgcgtccgc cgccgccgcg ctgcgccagg tgcggcacgc ctcgacgacc
    13321 gtcgtcactg tggcttggcc cgagtcggcc ttcgcgaccc caccgacctc gcagggctgg
    13381 ctggaagccg gggacgcccc ggtgagcggc gtgaccatct cgtcggccaa gtggccaggg
    13441 cgggccccgg ctggcgtcgt gctcgctcgg gcgttcgtgc ctgaccgggt gggccgtctg
    13501 gcgcgcggaa gcgacgggga gatcctcgac gccgtgaccc ggcatgtgag cggcatcctc
    13561 ggcgcccacc aaccgccgac cctcacccgg gtcacccgtt gggtcggggt catgccgaag
    13621 tacaccgtcg ggcacctcga tcgggtcgct gccgtcgacg ccggccttgc gggacaaccg
    13681 agttggaccg tcgccgggtc ggcgctgcgc ggggtcggca tacccgattg catcgccgac
    13741 ggacgggccg ccgtgagccg cgtgcttggt tcgcccaagg cgaccgcgaa cgccagcccg
    13801 acgaccacag ccacccccac gaccgccagc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nncggcagcc cacgcccgag aggacacccg catgagcacc caggagcagc
    13981 ccaccgcgac cgagacgtct gcccctgccg ccgctccggc agccgggcag gcgccagtga
    14041 cggcgcccgc gaccaaggtg acggcgcggg agatcaacga gacgatccgc tacacgtgct
    14101 gggccgtgtt cgccgccgcc gaagggcccg acgcgggcgc tgccgagcgg cttgccgagg
    14161 tggtcgccgg cctcgccgcg acggacgtcg tcgtccgtgg gttctacgac gtgtccgcca
    14221 tgcgcgccga cgcggacgtc atggtgtggc tgcacgggcc aaccccggag tcgatccaga
    14281 cggcggtgcg ggccatccgc cgacaagggt tcggaccggc ggcgaccttc gtctggtcag
    14341 gcgtcgggat gcaccgcccg gccgagttca acaaggggca tgtgccggcc ttcatgaccg
    14401 gcgctcccgc gcaggactat ctcgtcgtct atccgttcgt ccgctccctc gagtggtacc
    14461 tcctgcccga ggccgagcgc cgggcgatgc tcgtcgagca cgggatgatg ggccgcgcgt
    14521 ggccgcaggt cctcaccaac acggtcgcgg cgttcgcact cggcgactac gagtggatgc
    14581 tggccctgga aggccccgcg ttgcacgaca tcgtcgacct catgcggcac ctccgggcgg
    14641 ccgaagcccg acgccatgtc cgggtcgagg tcccgttcta caccggccgc ttggtgggca
    14701 tcgccgaagc cgtcgacgca ctgcggtgag cctcgcctac gacgcgctcg tcctcgtcgg
    14761 gttcgggggt ccggccgggc cggacgacgt catgccgttc ctgcggcggg tgaccgcggg
    14821 gcgcccggtg ccccaggatc ggctcgacga ggtcgccgcc cgttatctcg agctcggcgg
    14881 gagcagcccc atcgtcgccc agaccgaagc cctgctagcc gctctcgtcg agcggctcgc
    14941 ggcgcgcggt ctggaggtgc cggcatacct tgggcatcgc aattccagcc cgacggtgcc
    15001 cgacacgctc gcgcgcgttg ccgctgatgg gcacacccgg gtgctcgcgc tcgcatcgag
    15061 cgccttcccg tcctattcgg ggtgtcggca gtatcgcgag aacctcggcg ccgggttggc
    15121 cgccgcaccg ggtctagccg ttcgcgcgct cgggcccttc cccgaactgc ccggcctcat
    15181 cgacgccgct gccgacctgc tcgcgagcgc gctccggtcg gcggggccag gggcgaccgt
    15241 tctcgtcacg gcccactcgc tgccgctcgc ctcggcggcc acgtcgggtc cgaacggagg
    15301 cgggtacgtc gcggcccttg agcatgcagc ggcggcggtt gtcgcacggg ccacggccct
    15361 cgccggtatg ccgcgtccac ccgcgtggcg gctggtcttc cagtcccgca gtgggtcacc
    15421 acacgtgccc tggctcgagc ctgatgtctg cgatgcgatc cgcgagatcg cttctgacgc
    15481 aacgactcag accatcgtcg tcgtgccgtt gggcttcctc accgaccaca ccgaggtgct
    15541 ctgggacctc gaccgtgagg ctgccgaggc cgccgcggcg gccgggctcg gcttcgtccg
    15601 ggtgccgacg gtcgggaccc atccggcctt cctcgatggg ttggccgacc tcctcgccgc
    15661 ccaccttgag ggtcgtgccc cagccccggc gcagccggcc ttctgctccg accgctgctg
    15721 ccccagcccg gcgcagatgg aaggctgacc ccatgcgtcc gaacgcctcg ttccccagcg
    15781 cccgacctcg ccgactgcgc acgactcctg cggtccggcg gctcgtcgcc gagacccgtt
    15841 tgcatccggc cgggctcatc cagccgttgt tcgtcgccga aggcctcacc gagccgcgcc
    15901 cgctcacgct catgccgggg gtcgtgcagc acagcgtcga gtccttgcgc gtcgccgtcg
    15961 ccgaagcggc cgctgccggg gtgggcgcgg tgatgatctt cggcgtccca gaagagcgcg
    16021 acgcgaccgg ctccggcgcc accgacccgg acgggatcct caccgtgggg gttcgcgctg
    16081 cggtcgaggc ggggggcggg attcccgtca tcgccgacct gtgcctcgat gagttcaccg
    16141 accacgggca ttgcggggtg ctcgacaccc acggccgggt cgacaacgac gcgacgctgc
    16201 aggcctacgg cgacatggcc ctcgtcctcg ccgacgccgg ggcgcacgcc ctcggggcca
    16261 gcggcatgat ggacgggcag atcggtgcgg ttcgcgaggt gctcgacgcc gccggacacc
    16321 tcgacacgat cctcctcgcg tatgccgcga agtacgcctc cgccttctac ggcccgttcc
    16381 gcgaggcggt cggctccacc ctgacgggcg accgacgcac ctaccaacag gacccaggca
    16441 acgctcgcga ggcgatccga gaggtccggc tcgacctcgc cgagggcgcc gacatcgtca
    16501 tggtcaagcc agccctggcc tatctcgacg tcatctcacg agtcgccgcg atctcggacg
    16561 tgccggtcgc ggcatacctg gtgtccgggg agttcgcgat ggtggaggcg gctgcggccg
    16621 cgggggcgat cgaccgcgct tcggccatcg tcgaggtgct cacctcggtg acgcgagccg
    16681 gcgccgatct cgtggcgacc tactgggcga ccgaggtggc cggctggctc aacggaacgg
    16741 agcgctgacg tgaccagcag cgtcacttcg gaggagttgt tcgcccgggc ccgagccgtc
    16801 atgcccggag gggtgtcctc gccggtgcgc gccttcgggg cggtcggtgg gacgccgcga
    16861 ttcgtcgcct cagccgccgg aggcacgatc accgacatcg aggggcgcac ctatgtcgac
    16921 ctcgtcggcg gctggggtcc ggcgatcgtc ggccacgccc atccggcggt tgtcgaggcc
    16981 gttcaggcgg ccgtcgcgcg ctcggcgtcc tttggggcgc cgtgtctgcc ggaactcgaa
    17041 ctcgccgagg cgatctgcgc ccgggttgca cctgtcgagc gggtccgatt cacctcctcg
    17101 gggaccgagg cggtcatgac ggccattcgc atcgcccggg ccgccaccgg acgccgcgtc
    17161 gtgctgaagt tcgccggctg ctatcacggg cacgccgacg ccttcctcgt cgccgccggc
    17221 tccggggtgg ccacgctcgg cctccccgac tcccccggcg tcacgccggc ggtcgctgcc
    17281 gacacgatcg tcgtccctta tggcgacctc cacgccgttc gagcggcctt cgccgcccac
    17341 cccggccaga tcgctgccgc gctcaccgaa gcgatccccg ccaacatggg ggtcgtcgtc
    17401 cctccggtcg gtttcaacgc ggctctggcg caggtatgcc gcgccgacgg cgccctgctc
    17461 gtgctcgacg aggtgatgac cgggttccgg gtcggaccgg ccggtcgctg ggggctcgaa
    17521 ggtgccgaac agggttgggc accagacctg ttcacgttcg gcaaggtcat cggtggtggg
    17581 ctgcccctcg ccgcagtggc cgggccgggt gccctgatgg acctgctcgc cccggccgga
    17641 ccggtctatc aggcgggcac cctctcaggg aaccctgcgg cgtcagcagc gggactggcc
    17701 acgctcagcc tcctggacga cgcggcatac cggcgcctgg acgagtcggc cgcgacggtg
    17761 gcggcgatgg tgagcgccca cctggcggca gccggggtcg cgcatcgggt caacgcggcg
    17821 gggaacctct tcagtgtgtt cttcaccgcc gacccggtga ctgactatga gtcagccaag
    17881 cgccaggaca ccgcggccta cgggcggttc ttccacgagt tgttggaccg cggggtgtgg
    17941 ctgccgccga gtgcctacga ggcgtggttc gtgtcgaccg cgcacaccaa ccgcgagttg
    18001 tcggtgctgc aggcggccct gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18121 nnntcgtgtc gaccgcgcac accaaccgcg agttgtcggt gctgcaggcg gccctgcctg
    18181 ccgctgcgcg agccgcggcg ggctgacgcg agcaggtcag cggccagagg gttcagcggc
    18241 gagctgaggt ctgctcgccg ctcgtcgccg cctcccgagg cgcacggagg gctggaaaga
    18301 tccggtgcct caggtacgca gtccggacct gaggcacggg atttctccag gggtcctggg
    18361 cccgtcacga cgggagcacg ctcacgcggc tcagaagcgg tcgaggaagt cgtcgagcac
    18421 ccgcgcggag aactccaggc tcgacaccgg cacccgctcg tcgacgccgt ggaagagcga
    18481 ccagaaatcc agcccctccg gcagcagcac ggggttgaag ccgaagcact cgatgcccaa
    18541 ccgctgcagg tgggtcgcgt cggtgccgcc ggagagcatg atcggcgcga ccgaggcgat
    18601 ggcgtcctgt gaccgcaggg cctgggtcgc cgccgcgaag aaggccggcc cggcccccgc
    18661 cgtcatcggc gtcaagccgt cggtgcgggc gagttcgcgc acctggaccc ggtcgccgag
    18721 cgccgcgtgc aactccccga ggaactcgtc ccacctcccc ggcagatagc ggcagtccag
    18781 ctcggcccag gcgtcacccg ggatgacgtt gctctggacg cccgcgcgta gccgggtggc
    18841 atgtgccgta tgccgcagcg ccgcagcgcc attggcggcc tccgtgccga gcaggtgcag
    18901 ggaggcttcc ggatcggccg ggtcgaaggt tgttccggcg gcctcggcgg cgacgcgcag
    18961 cagtccctcg atctgggtgg tgaggtggat cggaaaacgg tcgtgggtga gccgccccaa
    19021 cgcctcgctg agcacccgaa gcgggttgtc gtcgtagacc cgcgacgcgt gcgccggcgg
    19081 ggcggttgcc gtgaccgaca ttgcggccca acccttttcg gcgacgtgga gcggatagac
    19141 ccgttgtccg ccggcgagcg gcacactcca cccgccgcct tccccgatcg cggtccgaca
    19201 gccctcgaag agctccgggt gggtcgccac cgcccactcg gcccccagca caccggcgtt
    19261 ctcctcgtcg gcgaagaagg ccagcaccag gggacggcgt ggcaggtgac cggtccgcag
    19321 ccgccgccgg acgacggtga gcacggtggc gaccatgttc ttcatgtcga cggcgccgcg
    19381 gccccagacc cagccgtcgg cgatctcacc ggacaggggc ggcaccgacc agttcgcggc
    19441 ctgcgccggg acgacgtcga ggtgcatgtg gatgagcaac gggtcgaggg tcgggtcggc
    19501 accgggcacc ctggcaacga cggtgcttcg ccctggctcg gactcgataa gcaccggcga
    19561 cagcccgtcg tcggtgagcc ggccggccac ccactcggcc gccgtgcgct ctccgggacc
    19621 gccgatctgg gtgtccagcc ggatgaggtc cgacaacagg ggcgcgacgc cctcccacca
    19681 cggccctggg tcgaccaccg ggtggccgct cacaggtcca gcaccaatcg gtcgccacga
    19741 gcgcggccca cgcagatcat catggtcttc ccctcggcct gttccgcttc ggacaacagc
    19801 gagtcgcgat ggtccggtat gccgcccaac acggtcgtct cgcacgttcc gcagatgccc
    19861 tcttcacagg acaccggcgg gaagatgccg accgcttcga gcgccttgac aatcgtcgtt
    19921 ccggccggca ccgcgaccgt gactcccgag gccgctgcct cgacggtgaa catcgtgtca
    19981 ttggcgctca ccgcagggtc gacgggcagc gggacgaaac gctcgacgac gagcgacccc
    20041 gccggccagg ccgcggtcgc ttcctccgcc gcgcgcagga gcggctcggg tccgcagcag
    20101 tagaccaagg tgccgggtcg aggatcgcgt aacacctcac ccaagggcag caggcccagc
    20161 tcgtcctgcg ggtggatcag gacgtgatct ccgtatgccg cgagctcgtc gatgaacgcc
    20221 atcgacgggc gggttcggcc gccataaagc agcgaccact gggcgccggc cgcgtctgcc
    20281 gccgcgacca tcgacaggat cggggtgatg ccgatcccgc cggcgatgaa gacgtaggcc
    20341 gccgacggac gcaggacgaa gttgttccgt ggccctcggg cggcgatgac ggtgccgggg
    20401 cgcaccttct cgtgcaccgc ggcggagccg ccgcgcccac tctcctcgcg cagcactccg
    20461 acccgccatt gcccggaccg gtccggcgac ccgcagagcg agtattgccg aacgatcgag
    20521 ccgggcaggt gcaggtcgac gtgcgcgcca ggctcccacg cgggcaacgc ggacccgtcg
    20581 gcgttctcca agacgacgga gacgacgccc tcactctccc aacgaagttg gcgcaccagg
    20641 agggtgaggt cggattcgac ggcggtgggc acggcggctc cttctgacgg ttgggactca
    20701 gcgaaccgcg cgcaggaaga cgcgggtccg ctcgtgctgc gggtcgagga tgacctggtc
    20761 cggtggcccc gactcgacga tgacaccgga gtcgaagaac acgacccggt cggcgacgtc
    20821 gcgggcgaac cccatctcgt gggtgacgac ggccatcgtc atcccgctcg ctgcgaggtc
    20881 cttcatgacg gccaggacgt ccccgaccag ctcggggtcc agggccgagg tcggctcgtc
    20941 gaagagcatg aggctcggct gcaggcacag cgctcgggca atggcgacgc gttgctgctg
    21001 ccccccggag aggtgcctgg ggtaggcctt gatcttgtct cgcagaccga ccttgccgag
    21061 gagcacctcg gctcgggcag tgacctcctc ccggtcgacc ccgaacaccc gacgaggggc
    21121 ctcgatgagg ttgtcgagca ctgtcatgtg cgggaacagg ttgaagtgtt ggaacaccat
    21181 cccgatatgg gcccgctgcc gggcaatggc ccgctcactc aactcgtgca ggtggtggcc
    21241 gcgctgctca tagccgacaa cctgcccgtc gacccagatc cggccagcgt caacccgttc
    21301 gaggtggttg atgcagcgca ggaaggtgct cttgcccgac cccgaggggc cgatcatgac
    21361 gaccacctca cctcggtcga tcgtcaggtc cacgcctttg aggacctcgt tgaggccgaa
    21421 cttcttgtgc accctctcgg cgatgaccat ggggctcttg gccgacccga ccgtcggctg
    21481 ggcggccgtt gggtcggcgg gccggtcggc tggccggtcg gtcggtacgg cggtcatgag
    21541 gttcccctga cgcttggggc atgcgtgcgc agcaggcgcc gcaaccgttg gatcggggtc
    21601 tccggcaggg tccgttcacc tcgggcgaat cggcgttcga ggtagaactg gccgatggtc
    21661 aagatggtgg tcacgacgag ataccagatg ctcgcgacga tgagcatcgg aatgatctgg
    21721 tagttgcgcg ccgagatgat ctgcgcggag tagagcagat cgtgcaccgc catgaccgag
    21781 acgagcgagg tcgtcttgag catgccgatc gtttcgttgc ccgtcggcgg gacgatgact
    21841 cgcatggcct gcggaaggat gatccgccgc atcgtctcca tccgggtgag accgagagcg
    21901 ctcgcggcct cctgctgtcc gtggtcgacc gagaggattc ccgcgcggac gatctcggac
    21961 atgtatgccg cctcgttgag tcccagcccg aggatcgccg ccaccgtcgg cgagatcaac
    22021 tggttggcgt cgagcgcgac ccccggcagc ccgagagtga tcacgggata cagggctgcg
    22081 aggttgtacc agaagagcaa ctggacgagg acaggcgtgc ctcggaaggc cgcgacgtag
    22141 gcccacgcgg ccccgctgac gaccgggttg gccgacagcc gcatgaccgc gagcaacgcg
    22201 ccgaggacga cgccgaccac catgcacgcc acggtcagac cgagcgtcat catgagcccg
    22261 ctgccgatgg agacgtcccg caggtacgtg ttgaccgtgt cccaaccgaa ccgctcattg
    22321 cccaggacgg agatagccat ccggaccacg atgatgagga tcaccgccgc ggccaaccac
    22381 cgccccgggt gacgcttcgg gacgacggtc agctcctcgg ccggcgtcag caccggcacg
    22441 gcactcattt gatgatctcg ccgatcttcg cctgggtggc gaactgggtc ggcaggcccc
    22501 acttggccat gatcgctgcg agggtgccgt tcttgtcgag ggccttgagg gcggccccga
    22561 tcggggcggc cagcggcgag tcgttgggga cggcgaccgc gacgttggtc gggttgtagt
    22621 cgggtccggc cgcgagttcg agggtgccgg tcgccttggc ctgcaacgcc agggacacgc
    22681 tgtcggccat gacagcgtca gcccgacccg aggtcaccgc gaggttggca tcggtctgcg
    22741 cggggtagag gtcggcagtg atggcggcct tacccgcggc cgtgcaatcc gccgagaact
    22801 tcggcaggta gtccagcccc tggatggtgc ccttctgagc cgcgatcgtg cgaccgcaca
    22861 gggtcatcgg gtccatcgac agggccttcg ggtttccctt cggcacggcg atcccgctgc
    22921 caccggcgcc atacgtcacg aagtccacga ccttggcgcg ctcggcgctg gccgagaagg
    22981 ccgacatgcc cacgtcgtac ttctggctgg tcagcccgat gaggatcgag tcgaacgcgg
    23041 cgttgacgtc gttgaccttg atccccatcg tcgtgccgat ggcctgcgac aggtcggcgt
    23101 cgaaaccgat gatcgtcttg ttgtcggtgt cgaagtactc aaacggcgcg taggacgcgt
    23161 ccatggcgaa gctgagctga cccttggcct tgatgtcagc cggaacgagg gcggcgaccg
    23221 ccgggtcgag ggcagccttg cccgccgcgg aaccggccgt ggtcgatgtg gcggccactc
    23281 cggccgcggg cgctgtcgtg gtgctggtgg acgcctgacc cgcgttcgtg caggcggcga
    23341 gggcaaggga tgaggtggca aggagcgtga caagcatgag cgggcgcatc gggtcgtacc
    23401 tttcgatgga ggttggccgc tgggtcaggg gacccagcgc agcggtgcgt cgttcagggt
    23461 gttgaggcgg acgatggcca ctcgggccgg tccaggtgca ctggagaggt tgacggtctc
    23521 tgactcctcg ccctcgagga cgaaggcatc cccggggtgc agccagcgct gccagggacc
    23581 ggctgagtcc gcgacgaggt ggtggccgac gagtgggacg acgacgacct cctcaccggc
    23641 acgtcggctg acggtgacgg gaccggcgtc aacgaacgac gtggtggccc ggacactgtc
    23701 gggatcggtc tcgacgaaga cctccccacc cgagcccgcc gccggaggac cacgccatat
    23761 ccgccagcgc cagtcctcgc cgcggtcggc ggtgtcagga cacgctgcga gggtgaccga
    23821 cccgtcggct gccggcgtca tggcgttgtc gcggacgaga agccacacag gtcagccgac
    23881 cgaggccgtg gccgaggccg caggcggagc ggcggcagca gcggcggccg gcgacgcggc
    23941 atacctggcc tcgctggtcc ccgacgcggg gccggcggct gcgacggact cggcggcgag
    24001 caactgcgac atgacgcggc gcgccttggc gaccccggcg tcctgcgcga tgaggacgtc
    24061 gtgctcgccc ttggggcggg tctgcagcgc caactcctgc agtcgcagcg cctcggcgtc
    24121 ctcatcgagc acggtgccca ggccgatcct caggtcctcg gtcgccgcgg tgttctcgat
    24181 gccgaagttg cggcagaagg cgtagtagta ccagcaggtg tgctcgtcga tcggcgtgat
    24241 gccgttgaga accttgatga ggtagccctc ttcgcgcggc cgaccccgtc cggtgatccc
    24301 cgagtgcagg gtgtggtgac tcggaaccgc gaactcggtg gcgtggaacc ggtcgaagag
    24361 cccctcgacg cccatcgtcg aggcgtagag cgggggcgcc tcgacgcccg gcatgagtcg
    24421 gtcgaccgtc accgtcgtgc catcgacctc caccgtgatg ccgtgctcgt agatgtagtc
    24481 gtcgccgacc gtcgtgcggt ggatgaacga ctcgtgggtg aggtccagga ggttgtcgtg
    24541 gaccagatcg gcgcggcagt tgaacaggta ggagtgggtg accgtcgccc atgcggggtc
    24601 gttcatccag tgggtgtccg ggatggtggt cacatcggcg cgctccgctt caccggtcca
    24661 gatccagacc cagccgtcct tctcgtgcag cgggtaggcc cggaccttgg accgcgacgg
    24721 gatcgtcgac tgggccggca cccgggtgca cacgccatca cagccgaacg cgaagccgtg
    24781 gtaaccgcat tcgatcgagt cctcgaccag tcgcccggcc gagagggggt agccacggtg
    24841 ggcgcatcgg tcggccaacg cgaccgcgga gccgtccttg gcgcggtaga ggaccactgg
    24901 ctgctggcag atcgtcctgg cgaccgggtc ccgggtgact tcgtgagacc aggcggcgac
    24961 ataccaggcg ttgagcacgt gcggcatgcg gagactcctt cggctcggct accgccctgc
    25021 ctagccagca ggactggatg cactgtatac attggataca gtgcaaggca aggcttacct
    25081 cactcgggct tcgggcggtt gctacgctga cccggcgacc gcgcaggagc gggcccgagt
    25141 cacaaacccc ggaaggtcag gtggcaggaa tgggatccgt cagcgctgac cgctcccccg
    25201 gcggtcctcg cgtcgccctc ccctcggcgg ccgatctcgg ccggcgggga cgtcccatgg
    25261 gagccgtggt ctacgaggcc atccgcgacg ccatcgtcga aggccggatc gccgccgacg
    25321 agcaactcgt ccaggaacaa ctcgctgccg agttgggcgt ctcgcgcacc cccgtccgcg
    25381 acgcgctcaa ccgcctctcg cacgaaggtc tcgtccgcgc agtgctcggc cgcggctatc
    25441 aggtcatcga cctcaccgac caggacatca acgacgtgtt cgtcgtccgc gagcgactgg
    25501 agatgctcgc cgtcgaacag tcagccggcc ggctcaccgc gctgcatcag gcccgggctc
    25561 gcaccttgat cgaagagatg cgtgcgaccg accccgccga tgcggtcgcg caatatgagc
    25621 tcaaccgccg cttccaccag acgatcatcg agccctgcaa caacgcgatc ctgctcgggc
    25681 tgctcgatca actgtgggac ctcccggtca gccggcggat caccaagtcc taccttcgcg
    25741 acgccgccaa cgttgcccac atggtcgacg agcacgacgc gatcctggca gccgctgccg
    25801 aaggtgacct gccccgcctg ctcgatcttt cgcgggccca cctcaccctc ggttacggcg
    25861 aggcgcgcgc cgactcccag ccgcccacct gagcgccggg cgttcaggca tgattcgccg
    25921 tgggtgacca acgcaccacg cccgcgacac acacaacacg caaaggcggg gcttgatgca
    25981 gcacgagcgc gaggagcagc acgggcatgg gcacgggcat gggcacgggc atgggcacga
    26041 ccaggaccat ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcacggg
    26161 catgggcacg ggcatgggca cgggcatggg cacgaccagg accatggtca tggacacgag
    26221 cccgacgccg cgaccttctt cgacgaggtt gccgccacct gggacgacga cccgaagcga
    26281 gccgaacgag ccacagcgat cgcggacgcg atccgggctt ccgtcccgct cacccgccag
    26341 atgtccggct tcgagttcgg ttccggcacc gcgctgctca gtcgctccct ggccccggac
    26401 ctcgggccga tcacggtgtc ggacccctcc cgcggcatgc tcgaggtggt cgcgcaacgg
    26461 ctcgcggccg aaggcgacac ccagctcacc ccgctgctgt tggagcttgg ggtcgacccg
    26521 attcccggcg gggcggcata cgacctggcc tacggcatgc tggcgctgca tcacgttgac
    26581 gacgtgccgg ccgccctgcg ctccctccgg gcggccctgc gcccgggtgg gtatctgtgc
    26641 atcgccgacc tcgacgccga tggcggcgcc taccacgcgg gacacagcca cttccatggg
    26701 caccatggct tcgaccgcga ggcattggcc gcgcagctga tcgagggggg cttcgtggac
    26761 ccacacctgc gcaccgtcta ccacctgcgc aaaccggtcg acggggtcga gcaggacttc
    26821 ccgctcttcc tcgcggtcgc ccaggtcggt caggtcggtc aggccggccc ggaccgggcg
    26881 tagcctgcca gggcgacctt atcggggcga tgccgtccgg caggtcccct ggagtgggct
    26941 gtgagaacgg cgtacctcaa gcacgcagcc cgtacctgag gtacagattt ctacggggcg
    27001 aatggctacc gcgagagcct tctccgtccg cctcgatagg caggccccat cgtgagagac
    27061 tttttgggga aggcacccat gaggaacgcg tcttctcaag gagttgtgga tgaacgccgt
    27121 catcgtcgtc tcaacagcag tcatcgttgc cctcactgcg gcggctctcc tgcagttcgc
    27181 gctgaccaag agcggctcga tcgaccgcaa cgccatgatc ggcatcaaga cgtcggcgac
    27241 gcttgcttcg gattcggcat ggaaagcggg ccatgtggca gcgcagccgg ccctcatgac
    27301 tgcggccgtg acgggagccg ccgcactcgt cactgtcctc gtggcctggc tcctcgcacc
    27361 gcaagcagag ttggcgttag cggtcgtctc agccaccggt ctggtcgtga tgctcggcct
    27421 gttcgtccgg gttgcgttct ctgccaacca cgctgcgagg aacgccgtct aggcgttctc
    27481 gcgcggcgag gtcaggcctc ggcggacgcg gtgagttcgg cacgctggac gtgccggaac
    27541 ctagccacga tgatggcgag cgcggccgcc cccgcgagga tcggcacggc atacgcgcgc
    27601 tgggcacccc acgcctcgat cgcccagccg cccacggccg caccgcccga gatgccgatg
    27661 aggatgccgg tgatcgcgac ggccatcccc tcggtcacca tcgccttggg cacgagcacc
    27721 tgggccaagg acaggctcgt gatgagcatc ggcgcggtcg ccgaccccgc gacaaacatg
    27781 accacagcga gcggccagag gtcactcatg aagaaggccg gcgcttcaag cacaaacatc
    27841 ccggccgctg cgagcaacag cctccgggag agcgtcgagt tgaacttccg gcttccgaag
    27901 acgatgccgg cgagcgtcga cccgagggcg aacccgccga ggacgacgct cgaaaaaccg
    27961 gtattgccac gcgcatcggc gaaggcgacg gcgatcacct cgttggaccc gaagatgacc
    28021 cccgtcatcg tcaacgcggt cgcaacgagc atcagaccgg gccagcggat cgcgaggccc
    28081 gcgggccggt tttcgtgggc gacgactggc ggctcggtcg cggtcgcggt gaggaaggcg
    28141 accatcccca cagtgaagag cagttcggcg atgagcagcc cggcctccgg gaaccacagc
    28201 gtcgagacga ggacggcgac gaccggcccg atgacgaacg acccctcgtc ggcgacctgc
    28261 tcgaaggaca tcgccgtgtg cagttggtcc gcgtcgtccc gatagatgtg cgcccagcgc
    28321 gccctcgaca tcgggccggg ctcgggcaac accgcagaca gggcataggc cacaaagatc
    28381 gtccacaccg gcgcgttgac gatcgatagg accgccgccg aggtcccgaa gatcgcggac
    28441 gacacgatga agggcagggc gatccgacgt tgtccgtgct tgtcgatgag ccgcccgatg
    28501 aacggccccg cgacggcgag cgtgccgact ccgaccgctg agatcgcccc cgcgagaccg
    28561 aaactccccc gccgctcgct gagcatgacg atgatcgcca ccccgaacat cgacccgccg
    28621 acgcgcgaca gcgccgtccc ggcgatgaat cgcagggcgc ctgggacgcg gaacaggggg
    28681 acataggcgg acagcacgag agactccgta gacgcagtga accggacgcc acgggcgccc
    28741 ggctggaccc cccagtgtcg ctgacgcggt ggtcccccga caaatgggta tgctgcgtcg
    28801 cctacaaccg cagggcccgc cggtcgctgg ccgcacccgg cgcgggcggc atacctgctg
    28861 gcctgccgcg ttgcgcgcac gatgggtggg gccgaggtgt tagcccatag accccggcca
    28921 gagtcgggag gcggcgtctc tcgcgatgag gaatccgacc acgccgacga cccaccagat
    28981 cgcgttcctc gactgtcgat cgagccagcg ggcgagcctg ccaagcgcgc cgtcgacccg
    29041 tgcgcccagc agcagtctgg ccgcgagcag gatgagcgcc ggcagacaca tgacgacgac
    29101 gtagctggcc agcgcagcca gccgctccgg tgtggatgcg accgaggagg tgagcatccc
    29161 gacggcgccg aggtagggca gcatgctggc cgcctcaacc aggcccgcgc cgacggcgac
    29221 tgcgatgacc gtccgagacc gggcctccgg gccgaggact cgatccctcc agccgggccg
    29281 ccggccgccg cccgcagcgt cgcgctcttg gcggcgctgc ttgacccatg gcaggtcggg
    29341 ccagaagctt cccgccaaga gggccagacc caccacgagt tgcaccatgt cgacgacccg
    29401 gcttcgctcg gtggctgcga ccagagccag gacgcgttcg gccgcgaaca ggagggcgac
    29461 gccgaggacg aagtagaagg ccgagatggc ggcgaggtag agcaccacac ggctggcgcg
    29521 aacccgcggc tgcaccagca tgagcgtggg caggaccagc gtcccgaaac tggtcgcatc
    29581 gaccagcgcg agaccgacga gggcggccgc aagttcgggc gtcatgtccc ttttgtaaca
    29641 cattcgtatt gccaacggcg agagatcgtg cgagggtgag ccgtgcccaa gctcgtggac
    29701 catgacgagc ggcgccacga gatcgcacgc gcgttgtggt cggtcgtcgc ccgaggagga
    29761 ttcgaggcgg cgagcgcgcg aaccgttgcc gctgaaggca attggtcttt gggcgcgctg
    29821 cgccactact tctcgtcgca ggacgagctc gtcgagtttg ctgcgcgaga gctcatcgac
    29881 ggcgccgccg cgcgcatcgc gaccgtcctt gaaacgatgc cagcggggtt gccgcgatgc
    29941 cgaatggccc tcgaacaact cctccctctc gacgacgttc gccgcggtga agttcgcgcc
    30001 tggctcgcga tcctggtcaa ggccgaggtg agccctagcc tggcggaact gcgcgaggtc
    30061 gcgctggacg gcgagcgcca actgtgtcgg ttagtcgtcg ccgaactcag cggccacccc
    30121 taccccacct caccgagcga ctggccggcc gccctcgagt atgccgccgt cgaccttcac
    30181 gcctggatcg acgggcactg cctacaggca gcgtcgtccc accggttgtt gcccgccgac
    30241 gctgcccgtg cggctcttga tcgttacctc cgccaactct ccgtcaccgt cgccccaggc
    30301 accgcctgac ttccgaagtg aagcacttca tgcagtaaag cccgccgatg cgtgatgagt
    30361 cgagtcgggc gcctgtcaaa cccgcgccga tgcggaggcg cgaacactgc gcagcagggc
    30421 agcgagttga gggatcgccg cgggctcggc gtccagcgcc cgctccaact gcagcacttc
    30481 atgcgggtca aggcgcaaca cgcggtcctc ggccaacacg gtccgagccc ggtccaacgc
    30541 cgcgccgaga atgaaagaag tcaaatcttg gtcctgaacc tcggccgcct cacgcagagt
    30601 ggcgagcgtc gccgatgagc aacgcaggtt gagtcggtcg gtcttggtgg acatgctcgc
    30661 tcctggatca ggaggggttt cgtgcgcccc ttgtacgtat accgtactca ctacggtaac
    30721 acgtctagcg cggatgccct aagcgttccg cctcaaacgt ggcccgcaac tgcttcgtgg
    30781 gcatgagcag atgccgggga tgagagacga aggccgtgaa gccatagcgc gcgtagaagg
    30841 tcacggcact ctcgtccgcg gcgtggacga cgacgacttc gaacccgacc gagcggctgg
    30901 cctcagcgat ctggcgcagc gcatgcacca agagcgttcg cccaactcct ctgcccgccc
    30961 actcgccgtc aaccgccagc cgagccagga ggacggccgg caccgggtat cgacgcttgc
    31021 ccgcacccag agcccgagcc gcctcgtcga gttccaggcg gtacgtcgtc gtcgcgtagt
    31081 agccgatcac ccgttcgcca cccgggtcga gcgcaaggaa cgtccgggtg ttgtcagagc
    31141 gttccccctg gccggccttg gtcctgagcc agtcgtcgag gtcagcattg ccacttgtga
    31201 accgcgagcg atcaaagctc ttgccgaagg caacgatggg gacttcaagg gcgatcacga
    31261 gtcactccac atcgaagact ttcatcgatt gttcgccaac aggctcactc aacacggcgg
    31321 aatggctgcc cggatccccc accctgaggt cctcgcccag cgagtcggtc gacaccaaag
    31381 ccctcctcgc caatcttggg caggccgtgg ccggagtggt cacccctatc cacacaccct
    31441 tgttcatccc cgcgtcaggt gcaacctcaa cacgcctcgc tcggcagcac ccatccgaga
    31501 tcgcttcggc cgcacgctct cgccatgagt taggtcaccc acccccgcgc gggacccgat
    31561 gtcgtagcat ccaatcgata attctcagtt gaattctttg gaggcgacgg tggcacatcc
    31621 aggcgggcac ggtagtcatc ccgcgcggac ggggtggctc aacgcgggcg caacgcttgt
    31681 tgccggagtg atcggagctg tcgcaacact gctggcggcg cgctcgggca atctgccttc
    31741 cgtagtggct cttcccacca ccaccatcac ggtgacgcaa acggcactat ccacgacgac
    31801 ggagaccgtc acccgcacgg tgacgcctac catcggctcg aacaccggga ctggcgttgc
    31861 agccaccggc aattctggcc gaacgtacct cgttgatctt cagcccgtcg actcatctct
    31921 cgaacaacgg gtcgtggact tttccgacgt cacctaccca aactccctta tcaacctcat
    31981 gtcgggatgc tcccaaacag gacccgtcga ctaccttgtc cccggaggag cgactcaact
    32041 agtcgcggag gtcggcgtaa cccgggactc acttgagcca gactctcggg ttacattcca
    32101 agtaacggtt gatgggcagc gcgccgaacc gatcaccgcc caagtcgcgc agcacgcaac
    32161 actcagagta gtggtgagca gtaagagccg cgtgcggctc gagacaagca tcgacaagaa
    32221 ccgccgcaca aactgcaata cggaggcagt cgccgtctgg ggtgacccct acttcagttg
    32281 aggcgccgca cgggcttgct ataaaagcgc cgttccagga ccaccaacgc gagagtggct
    32341 ggccgccatc aggacccccc atcgtcgagc tgcgcatgtg agcccgatcg acacgctcac
    32401 ggggtcggtc gtcaccaagc cgccgtggta ccagcgtcaa tgcgccgtgc ggcacccata
    32461 gaagtatgat cgtactgcta tgctcttggc aggcccccaa tcaaggagtt ccgcggcatg
    32521 acgaggtacc gccccgagca caaggaggcg acccggcgca ggctgatcga ggcggccggt
    32581 cgtcggttca agagcgacgg catcgacggg tccggcatcg ccacgctggt tgccgacgcg
    32641 gggctcacga acggcgcctt ctacaaccac ttcgcgtcca aggacgacct gatcgccact
    32701 gtcgtggccg cgcagttgac ggaccaggcc gcggtcatcg acgcgttgcc ggtaggcccg
    32761 gcctcgatgc agacctatct gcggcagtac ctctcccccg cacaccgcga cgaccgcgcc
    32821 ggcggttgcc cgtcggccgc cctgctcgac gagatcggcc gttgcgacga ggcgacgcgc
    32881 gcggcataca ccgtcggtgt cgagccgatg atcgcggccg tcgcgcggca ccttgacgac
    32941 ggcgatccgc aaggggcgca ggagcgcgcg atcggcttgc tcacactgct cgtcggctcc
    33001 ctgcagttgg cccgcgccgt cacgaatccg gcgctgtccc aacagatcct tgccacggca
    33061 tacacccatg ccttgtccct cgcgggcgtc cagccgggcg cctccacact gcccgtcgca
    33121 ccccccaccc aggagtcgtc atgaaggcct ttgtcgtcac ccactacgga cccgacgggc
    33181 tggcagccgc cgaggtcgcc acgcccaccg ccgggccggg cgacgtgctg gttgacatcc
    33241 gcgcggcgag catcaacccg ctggacaaga tgatccgcaa cggggagttc aagcacctca
    33301 tcaagtacaa gccgccgttc gccctcggcc acgacctctc cggcgtgatc accgccgtgg
    33361 gctccgacgt gcgcg
//
LOCUS       DAORPW010000009        32403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_198_length_32403_cov_727.983492, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000009 DAORPW010000000
VERSION     DAORPW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 32403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32403
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198_length_32403_cov_727.983492"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2139)
                     /locus_tag="PLB86_01745"
     CDS             complement(<1..2139)
                     /locus_tag="PLB86_01745"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HRC63890.1"
                     /translation="MTRRGANPALAALASLAALLGLTTLVVTQTWYARTIWFVFVAVG
                     IGALIRRFVSRSGWLIVPAQVLGVLLMATWQYAGSTTWWGVPTWSTVGRFTELFTLFG
                     TTVAESSAPLLANVGVEVTLALIGAGVAILVDALAVTRAAPAAAGLPLLTAFLAAAAN
                     SGSALPPAYFIIAALAWLLMLGRQAQVGMRGWASAAARPMAPISGAALDSAPELRFGT
                     IARRLGAVGLVAAIALPAVLPHLPPRYLLDGLARSADGAGRGVGKVGFNSTLDVGLSL
                     TSGGTGTILTYRTNAPAAAPLRVLAATAYDGTNWSRPAPTLGRSARLDLADTVVRSER
                     TIVVESNALDPPSLATPQPILSADLSGVSWQVDVATSDVSVQTRPNAYSTTYLEPVLT
                     ADLLREGIDGRPGPDRLPANRTMSAALAVDPRSEAAVRALTTTVTAGAATPYDEAVAI
                     QEWLRSKGGFTYSLTLPEAPLVSGVVGDPISAFLQTKTGYCVQFTSAMVMMSRAAGIP
                     ARVAIGFLPGSQDGGLWTVTAGDAHSWPELYFPGAGWVRFEPTPSVRTGTAPSWTVPP
                     VTPTPLATSTARSTEDPLDRPDRDAPDDADDTTTARTELDLPILDRVLSWVAQIPHLF
                     LLVVGLVVVATLVLPVTAALLRRVRSRRGGPPNEVIEAHWELFTSELHDLGITTPAGG
                     TLRDSQAHVARAGYLGEPARQSLATVVDTVE"
     gene            complement(2136..3431)
                     /locus_tag="PLB86_01750"
     CDS             complement(2136..3431)
                     /locus_tag="PLB86_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC63891.1"
                     /translation="MRRFGEVLTTRGRAFCSAGITLMVCGYVLGFRDLTRVGVLLLGL
                     PLLAGLLARRRPPGLVVTRTSDPVQISAGEATTVTTTVANGGHRSTPLMLAEEQLDHV
                     LGERPRVLLGSLGAGERRALTYAVRPPLRGRHLLGPLTVQLRDPFGLSQRFVEAGEPT
                     EVLVLPRVYPLGGNRPPGTGVGAEGEIPFMVALHGEDDQSIREYRDGDDLRRIHWPAT
                     ARTGELMVRQEDRPARRRAIVLLDPRVDAHAGVGIGSSFEWAVSAAASMVVHLSGLGY
                     AVHLLTPETVGDGPDALPIDPAQALAVLAVAEPDTGLALARLTRAALGLLSGGGLLVA
                     VLAAHDESALRSVATLRHPGATALGMVLDTGSFAGRPAGGGANAGGGAVAPEAARAVD
                     LLRGAGWRATTVTRDDSVEHAWSQVGSRRDAQVALGASR"
     gene            complement(3465..4433)
                     /locus_tag="PLB86_01755"
     CDS             complement(3465..4433)
                     /locus_tag="PLB86_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC63892.1"
                     /translation="MVRTTPEALAELRAVTSRIASAMESVIEGKPAVVRTAITVLLAG
                     GHLLIEDVPGVGKTMLAKALARSIDCSVRRIQFTPDLLPSDITGVSVFNQDLRDFEFR
                     PGAIFANVVVGDEINRASPKTQSALLECMEESQVTVDGTTYSLQRPFIVMATQNPIEM
                     EGTYPLPEAQRDRFMARISMGYPSTAAEVEMLDLHGGRSPLDTLVPVTDAQTVARLVL
                     VTQDVYASPAVRQYVVDLANATRTSPAVRLGASPRAALHVLRAARAHAALEARDHVLP
                     DDVQRVAPAVLGHRLMLSGEATLAHRSPAEVLAEILRSTPVPIGRR"
     gene            4967..5407
                     /gene="mraZ"
                     /locus_tag="PLB86_01760"
     CDS             4967..5407
                     /gene="mraZ"
                     /locus_tag="PLB86_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRC63893.1"
                     /translation="MPTPFLGTHTPKLDDKGRLILPAKFREPLAEGLVMAKGQERCIV
                     VYPMAEFYRFAAQMQTAPSSVSGVRDYTRVLASSASPEVPDKQGRITIPPMLREYAAL
                     GRDLAVIGNISRVEIWQLDAWNRYLADKEPGYASQSEEVMPGRF"
     gene            5662..6651
                     /gene="rsmH"
                     /locus_tag="PLB86_01765"
     CDS             5662..6651
                     /gene="rsmH"
                     /locus_tag="PLB86_01765"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRC63894.1"
                     /translation="MTTSPERATSDRHVPVLRDRVLELLAPALAAEGAVYVDGTLGMG
                     GHAEGVLQAFPHARLIGIDRDPQALALAGQRLAPFADRITLVHAVYDEFAQVVDALGI
                     PTVDAALFDLGVSSLHLDETDRGFAYRVDAPLDMRMDQTRGMTAADVLNGYEPADLER
                     ILRDFGEERYARSIVRAIVADRAKEPYTTSGPLVDMLRRVVPGASQRSGGHPAKRTFQ
                     ALRIEVNGELDAWRRALPAAVDRLRVGGRVAVLSYHSLEDRITKRCLAEGATSRTPIG
                     MPVERPQDAAYLKLLTRGAEVPTDAEIHDNPRAASARLRAAERTRPTTQGEHR"
     gene            6738..7250
                     /locus_tag="PLB86_01770"
     CDS             6738..7250
                     /locus_tag="PLB86_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63895.1"
                     /translation="MPAAIERTGTGKFAAICMVLLTVGLLALLLLNTQLAQGSFRLHD
                     LQVASGTLTDQEHELTRALDAERNPGSLAKRALDMGMVPATSMAFIRLEDGVILGVAE
                     PAAAKVLTVVNAPKTQPSAPAPVAPDPAATPAPAATPAPTATPAPADAAASDPEVPDG
                     APVTATVPAG"
     gene            7501..9276
                     /locus_tag="PLB86_01775"
     CDS             7501..9276
                     /locus_tag="PLB86_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRC63896.1"
                     /translation="MWVAVLFVLSIFGAQLVRVQAFDASGVQLAALNKRLNSVVVPAL
                     RGRIVDTKGTVLAASVERRTITVNQNAVATYERVIEGKRVKVGVVGAAERLAPLLGMT
                     ADELVKKLMGTSGYQVIAKGVSPLVWREISAMGIPGILSEVTFEREYPSGMTGAPIVG
                     FLGDDGSVAGGVEQMMQAQLAGTPGKEIYEQARDGKVIPWAQRLSQPAVDGQDVRLTI
                     DADIQWFAQNTIGNLVHAMGAKSGTIVVMEAKTGKLRAVAPYPSFDPTDLAHTKASAL
                     GNKAFQEVYEPGSTAKVITIAAALEENVVEPMTGVIVPNRLRRADTTFKDAHDHPTLN
                     LTVAGVLAKSSNIGTILATESMPAATLEAYQRSFGLGTKSGIGYPAESAGLLRPAAIQ
                     TGSERYTTMFGQGMSVTAVQAAGVFQTIANGGVRVPPSLIEGTVAEDGTFAASTAPQG
                     VRAVSQQTSTEVSQMLEEVVGPGGTARHVAIPGYRIAGKTGTANRVADSGAGYSGYTT
                     SFIGYAPAEDPQFVVAVTLQNPTAGVPSGGSQCGPAFKDVMTYVLQAYQVPPTGAASA
                     EVPLVPAEPLVPGAPGVISDRKSTG"
     gene            9287..>10865
                     /locus_tag="PLB86_01780"
     CDS             9287..>10865
                     /locus_tag="PLB86_01780"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRC63897.1"
                     /translation="MHSHRPRSLPPVALETLADRAGATVVGGGSAQGVLVTGVSLDSR
                     TVRPGDLYAALPGAHAHGATFVAGAVAAGAVAVLTDPEGAPLAAIGLGVPILLVEAPR
                     AVLGDLAALVYGHPADAMRLIGVTGTNGKTTTAYLIESALRGLGARTGLIGTVETRVG
                     DERLTSRRTTPEAPDLHAILAVMREAGTDSVVMEVSSHALAQHRVDGVVYDVALFTNL
                     SQDHLDYHAGMADYFAAKAALFSPERAQRGVVCIDDEWGVALAQRRGIPLVTVSSRRP
                     ADWTLTAGEGPEVTLVGPGEQQLLLHCHLPGTFNLVNTAMAAVTLLQLGYAASEVERA
                     MALEPVVPGRMERVPGGPDDPRCVVDYAHTPEAVDAALAALRPSTAGRLVVVLGAGGD
                     RDTGKRAAMGAAGARWADDLIVTDDNPRSEDPAVIRDQVLAGARSVLPADPDGTTSAR
                     SLFSSDAGRAAHIERAVRLAREQGRPVDNTVVVLGKGHETGQDIGGTVHPFDDRDALA
                     AALRGAPYRPEGNDSGHV"
     assembly_gap    10866..10965
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11049..12449
                     /gene="murF"
                     /locus_tag="PLB86_01785"
     CDS             11049..12449
                     /gene="murF"
                     /locus_tag="PLB86_01785"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRC63898.1"
                     /translation="MDAHGRPAGPDAPEGRVLVDGPVVTDSRECRPGSLYVARVGEQA
                     DGHAFIGAAQEAGAVAALTSRPVGGVAHLLTDDVQTAFGAIARAVVDAAPDLTIVGIT
                     GSSGKTSTKDLLAQVLRTGAETVAPEGSYNGEIGVPLTVCRITPTTRFLVAEMGSRGI
                     GHIAYLTRIAPPRIGIVLNVGQAHVGEFGGRDNIARAKAELVAALPASGVAILNADDP
                     AVAAMATATTARVVLVGESAAANIRAEEVELDESGRPSFGVLSPAGTAQVRLRLHGRH
                     HVGNALSVIAAALECGLPLDDIVAALAVAGPVSRWRMEVTERADGVTIVNDAYNANPD
                     SMRAALRALRAMKGPADAGPGRRTWAVLGEMFELGPDSHTEHQQVGAVAVAQDVDRLV
                     VVGAGAGLIGDGAVAAGMPPAHLARVPDTDAAYALLTENLRRGDVVLFKSSRDAGLRW
                     LGDRVAETVHGNEVRS"
     gene            12446..13528
                     /gene="mraY"
                     /locus_tag="PLB86_01790"
     CDS             12446..13528
                     /gene="mraY"
                     /locus_tag="PLB86_01790"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRC63899.1"
                     /translation="MKGVLVAAATSLVLALLGTPWFIKWLVKRQYGQFIRDDGPTSHR
                     TKRGTPTMGGTVIVGATLAAYFLAHLVLGTWPSVSGLLVLFLMTGLALVGFLDDYIKI
                     SRQRSLGLRTKQKLFGQGLVGVTFAVLALQFENAGYRTPASTAISFVRDTRFDLAFAG
                     PAIGLVLFVLWANFIITAMSNGANLTDGLDGLATGASTMIFGAYLLIGIWTSNQNCQT
                     NPGLKCYEVRDSLDLAIVSAAAMGACFGFLWWNASPAKIFMGDTGSLGLGGLIAGLAI
                     TTRTEFLSVILAGLFVLETLSVLIQVSVFKATRKRFFRMAPLHHHFEMLGWEQITVVI
                     RFWIIAGLFAALGLGIFYAEWVVGST"
     gene            13525..15087
                     /gene="murD"
                     /locus_tag="PLB86_01795"
     CDS             13525..15087
                     /gene="murD"
                     /locus_tag="PLB86_01795"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRC63900.1"
                     /translation="MSELQDFADPIDDGYEPRPGLTHRDADWSGLRVLVAGLGISGFA
                     AADALWERGAVVTVVDASAPGASRTVDERAQILAVLGVDVRLGPEHTAGMPSDRAIDL
                     VVTSPGWRPTHPVLADAAAHGIPIWGEVELAWRMRAREGAAPWLTVTGTNGKTTTVNM
                     LASILRAAGLRATSAGNVGTPLLEAVLHPQPYDVIAVELSSFQLFWQRSLSPVASACL
                     NVAADHIDWHGSFEDYLRAKGRVFRNTHLACIYNVADPLTEQLVIDADVIEGCRAVGF
                     TLGIPALSMVGLVDEILADRAFVEQRHHSAAELATIADVQGEAPSIAPHYLANALAAA
                     ALARAYGVGPLSVRDGLRAFRPDPHRIAEVGTVDGVRYVNDSKATNPHAAEAALASFD
                     SVVWVAGGLLKGADIDALVEKVAGKLRGVVLIGADRALIAQALARHAPDVPVVDAGGP
                     DTGGMQTTGEDVDRVMDRVVAHAATLARPGDVVLLAPAAASMDMFANYGERGSAFERA
                     VGRFGERPGQRS"
     gene            15084..16385
                     /gene="ftsW"
                     /locus_tag="PLB86_01800"
     CDS             15084..16385
                     /gene="ftsW"
                     /locus_tag="PLB86_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRC63901.1"
                     /translation="MTSTVDRSARAPRAPRATRPGDTGAAHSWLPASLRPLAARFDSP
                     VTSYYVLVGVTTALLLIGLVMVLSASMITSYKDDGSSYAVFLNQARFAGIGLVGAFIA
                     SRLTVKWWKRLAVPSLAAAMVLQALVFVPGLGLSKGGNRNWLKLTSTLSLQPSELTKI
                     GLVLVGALILANKRASLGRVSHAVIPYLVPVAAITIGLVLAGHDLGTALVLIGIVGAV
                     MYAAGVPGRLFAAAAVGAALLATALVVTSSNRMDRIGNWLSGSCTDPNGECGQSVHGL
                     YALADGGWWGVGLGASKEKWSWLPEAHNDFIFAIIGEELGLPGTFVILALFALLASAC
                     YRIVTRSTDQFVRIAAAGAMAWILFQAMINIGAVIGLLPVIGVPLPFVSAGGSALVTT
                     LVAMGMLVSFARAEPGAAEILAARPGVIQRSLAVLPRRRAR"
     gene            16407..17549
                     /gene="murG"
                     /locus_tag="PLB86_01805"
     CDS             16407..17549
                     /gene="murG"
                     /locus_tag="PLB86_01805"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRC63902.1"
                     /translation="MTPFTRNRRARPADSGGELVSVLLAGGGTAGHVSPLLATADALR
                     RRRPDLTVTALGTQTGLEARIVPARGYDLRFVPRVPLPRKPDANLVRFPGALRAAVEA
                     AGRAIDETGAQVVVGFGGYVSSPAYLAARRRGIPIVVHEQNARPGFANRLGARLTPYV
                     ATTFAATHLAHATCIGMPLRREISTLDRAALRADARAELGLDADRTTLLVTGGSLGAA
                     RLNETMAARSRDITDAGIQVFHAAGNGKGFVPDVSPSGAPYIVREYVDRMDLAYAAAD
                     LVLCRSGASTVCELTAVGLPAVYVPLPFGNGEQRVNAADVVAAGGGLVVEDAALTPVW
                     VDVVLLPLLTDADRIARMGSAAATVGRRDADEALVDLVLAAFAGDR"
     gene            17546..19048
                     /gene="murC"
                     /locus_tag="PLB86_01810"
     CDS             17546..19048
                     /gene="murC"
                     /locus_tag="PLB86_01810"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRC63903.1"
                     /translation="MTLSSGLPPTRFDYAAPVPPIAELGAVHLIGIGGAGMSAIARML
                     LARGLLVTGSDSRESASVDALTRDGIGIRIGQDAANVTGVQTVVVSSAIREDNPELAA
                     ARAAGLRVMHRSQALAALAADSPVRIAIAGANGKTTTSSMLAVVLTLAGRDPSYAVGG
                     DPVQLDANARAGTGEEFVIEADESDGSFVGYHPHVALVTSVQPDHLDFYGTAEAVEAG
                     YRAFVDTLDPGGLLVACLDDPGARRLADLARSQGRRVVGYGFSADAQVRLSDARLGGL
                     GAEATIEVDDAAYPMRLSVPGEHNLLNAAGVLAVALFAVVASEPHGAGRSPERVIGWL
                     DLLSRFSGVRRRFERVGEAVGVVVVDDYAHNPAKVRAAVATGRGAVEAGGRLVVVFQP
                     HLYSRTRDFAAEFGAALAPADVVVVTDVYAAREDPLPGVTGALVADAVRGVRAGVQVE
                     FVPALGDVVPALTGLVAPGDLVLTVGAGDITSIGPALLLALGARSIREGQ"
     gene            19132..19836
                     /locus_tag="PLB86_01815"
     CDS             19132..19836
                     /locus_tag="PLB86_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRC63904.1"
                     /translation="MRFEQRAHAARRRPWVLVGTAVAILALMGGLGWVVWASPVLVVD
                     EVVVTGLDGADRDAALTAAAVPIGEPLARVDSAAVAQRVGSLPIVKRAVVTRSWPRTL
                     VVSVTARVGMLVVRNSEGTLQVVDEDGVAFRDVDQAPAGLAVVNGSGQRPAPEGLQAV
                     IEVLRILPADQRATVSAITVSSASMVTFALGEVEVVWGGRGSEEKKGKVLQVLLKTTP
                     APKVIDVSAPDTPVTR"
     gene            20145..21365
                     /gene="ftsZ"
                     /locus_tag="PLB86_01820"
     CDS             20145..21365
                     /gene="ftsZ"
                     /locus_tag="PLB86_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRC63905.1"
                     /translation="MGAAPQNYLAVIKVVGIGGGGVNAINRMIDVGLKGVEFIAINTD
                     AQALLMSDADVKLDVGRELTRGLGAGADPNVGRKAAEDHAEEIEEVLRGADMVFVTAG
                     EGGGTGTGGAPVVARIARSLGALTIGVVTRPFTFEGRRRANQAESGIGGLREEVDTLI
                     VIPNDRLLSISDRNVSMMDAFRSADQVLLSGVQGITDLITTPGLINLDFADVKSVMQG
                     AGSALMGIGSARGEDRAVQAAELAISSPLLEASIDGAHGVLLSISGGSDLGLFEINEA
                     ARLVQEAAHPEANIIFGTVIDDALGDEVRVTVIAAGFDGGDPTPRSDARALGQVQAGG
                     PGRPAAASGPSTAAAGGSSAGAAYASGPAATPAPAPAPAPAPAPVVVPPVQVPPRPVS
                     VDDADDLDVPDFLK"
     gene            21427..22197
                     /gene="pgeF"
                     /locus_tag="PLB86_01825"
     CDS             21427..22197
                     /gene="pgeF"
                     /locus_tag="PLB86_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRC63906.1"
                     /translation="MFFWHERRAGIDVAITDRHGGVSSAPFGELNLARHVGDEPAAVE
                     ANRAAVAAELGLPAERLVFMDQCHGATVEVIDGPWQGDPPACDAVITRASDLALVVMV
                     ADCVPVLLADPEAGVVGAVHAGRPGLVAGVVGAALDAMADLGARAPYAVVGPSVCGRC
                     YEVPEEMRAAAAAVEPASATVSWTGTPAIDVGAGVVAQLAGRGVPVTWVPGCTREDDR
                     LFSYRRTSPTGRFAGAIRRYAVDPAYLIERPSHPTETS"
     gene            22194..22979
                     /locus_tag="PLB86_01830"
     CDS             22194..22979
                     /locus_tag="PLB86_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HRC63907.1"
                     /translation="MTGFSPQPTEVGDQRAEALRAGLADVRERVARACDAAGRDPAEV
                     TVIVVTKFFPASDVRRLAALGVRDMGENRDQEASAKFADVKADTAGLRLHFIGQIQTN
                     KAASVASYADVVHTVDRLKLVTALDRGAAAADRRLRVLVQVDLDPARPAATLGAVATA
                     AASAGPARGGADPADVGGLVAAVQASAALDLLGVMAVAPLGVDPGPAFGRLADVSRAV
                     RAVVPDATWISAGMSGDLEVAIRHGATHLRVGTAILGSRPTLR"
     gene            23059..23523
                     /gene="sepF"
                     /locus_tag="PLB86_01835"
     CDS             23059..23523
                     /gene="sepF"
                     /locus_tag="PLB86_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HRC63908.1"
                     /translation="MAGALRKTMVYLGLAEEDERYGSYDEAYEDDRVSEHTAAVTPLP
                     SRPTSVASVVRDPDMGSLTRITTIHPRTYNEAKAIGESFREGTPVIMNLTEMDDADAK
                     RLVDFAAGLVFGLHGAIERVTAKVFLLSPAHVEVTAEESPAVRSPRSPFNQS"
     gene            23588..23869
                     /locus_tag="PLB86_01840"
     CDS             23588..23869
                     /locus_tag="PLB86_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRC63909.1"
                     /translation="MRGVWQVISTLLFIFFVFLIGRLVLDWVRVLARSWRPHGIVLVL
                     AEAVYTVTDPPLRLVRKIVPSITLGSIRIDLAFIILFLLTSTLMSLQPF"
     gene            24015..25307
                     /locus_tag="PLB86_01845"
     CDS             24015..25307
                     /locus_tag="PLB86_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRC63910.1"
                     /translation="MALTPEQVLNKHFQTTQFRKGYDERDVDDFLDEIVAEMRSLLAQ
                     RDDYKQQLADCRAGRGMSITDAATVAAPAFVDTRAADEAEARLVEANAELAQAQTEHA
                     RVLESIGSARAEFDGLQSRLDGLRGSLADAQAQADGGPRLVQDAEAAVADAQRRAEEF
                     GSRAAEAEKHLYEIEARAAEADAKAQEAAARVSEAEQRAAEAQASAADAQARAEQAEA
                     AAQAAAERERQSIEATRVEAPAAPAAALVGAGATGAGSAAGLIELAQRLHDEHVAQGE
                     AKRHELVSAAENRHNELLATGQARFDELVGSGQRQHDDLVATAQSRHDELVSTAQNRH
                     DELVGTAQRQHDEMLAEAANLKEQMLTEARERSTGMVAEAQQKKAAVLEELEGQRTSM
                     ERKIDELRGFERTYRSQLRSYFEDKLSELTNTGADASA"
     assembly_gap    25850..25949
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25950..26249)
                     /locus_tag="PLB86_01850"
     CDS             complement(<25950..26249)
                     /locus_tag="PLB86_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63911.1"
                     /translation="MTVEADRTADTALALAAFAGAALAGAAFAGAAFAGAAFAGAAFV
                     GAALAGATLADTVFAGSDVTVATFAGAALVAAVLVVAFTGVAFAGVAFTAAAFAGA"
     gene            26269..26658
                     /locus_tag="PLB86_01855"
     CDS             26269..26658
                     /locus_tag="PLB86_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRC63912.1"
                     /translation="MPPVREDEEPWTEAELREVRQEIERDIARGLADIATAEEDLQGL
                     IWDAGDGSGDDQADAGAKTYEREQEISLANISRDKLAQNRHALERLDNGSYGICEACG
                     LPIGKFRLQAAPRATLCRSCKEKAERA"
     gene            26631..27254
                     /gene="lspA"
                     /locus_tag="PLB86_01860"
     CDS             26631..27254
                     /gene="lspA"
                     /locus_tag="PLB86_01860"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRC63913.1"
                     /translation="MQGESRARLSAPSPRPTRVSGSRRLIWLFVGIAVVVYVLDQASK
                     AWALAALTNAPPQDLLGAFLRLYLVRNPGAAFSVGTGSTWLLTILAVVVLVVLIRASR
                     RLGSMAWAWAFGLILGGALGNLTDRLIRDPGFGRGHVVDFIDYNGWFVGNVADIAIVG
                     AALLIGLLALRGIGLDGRRDGAGGSGEADGSGEADGIPEPRGTERDG"
     gene            27247..28185
                     /locus_tag="PLB86_01865"
     CDS             27247..28185
                     /locus_tag="PLB86_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRC63914.1"
                     /translation="MGDARTLFVPDGLDGERVDAAIARLFGFSRTRAAELAADGMVTL
                     NERVSPKSARVSAGDVLEVVIPDAPSAGVELVAEPVPGMSIVHDDDDLVVVDKPVGVA
                     AHPSVGWTGPNVVSGLAAAGYRIATSGASERQGIVSRLDVGTSGLMVVAKSEYAYSRL
                     KHAFRSRTVDKTYHALVQGLPDPMTGTVDAPIGRHPAHEFKFAVMASGKPAVTHYEVI
                     EAFHGASLLEIKLETGRTHQIRVHLSALHHPCCGDPLYGADPKVSARLGLDRQWLHAV
                     GLGFEHPGTGEWVTFASPYPADLEHALDVLADSPAR"
     gene            28325..31882
                     /gene="dnaE"
                     /locus_tag="PLB86_01870"
     CDS             28325..31882
                     /gene="dnaE"
                     /locus_tag="PLB86_01870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRC63915.1"
                     /translation="MSTPPASERSFAHLHVHTEYSMLDGAARIDELLDAAVRMGMPAI
                     ATTDHGYVFGAYEFWSKARKRGIKPIIGVEAYLTPGTHRTDKTRVRFGDDPANKDDVG
                     GGGAYTHMTLLARTTGGMHNLFRMSSLASLEGHYFKPRMDRDLLSRFSGGLIATTGCV
                     GGEVQTKLRVGQYAAAREAAAELRDIFGAGNFYAEVMDHGLPIERQTQRELLRLAKDL
                     GLPLLATNDLHYTKAEDSTAHAALLCVQSGSTLMDPGRFKFDAEEFYLKSPAQMRELF
                     RELPEACDNTLLVAEQCDVSFTEEEGRYMPRFPCPEGESETTWFIKEVQRGLHKRYPA
                     GIPTAVQERADFEVEVILGKGYPGYFLVVADFINWAKDHGIRVGPGRGSGAGSMCAYA
                     MGITDLDPIVHGLLFERFLNPERPSMPDFDVDFDERRRGEVIRYVTDTYGEDRVAQIV
                     TYGTIKAKQAVKDAARVMGHPFAMGDRLTKVMPADVMGKSMPLSGIFDSSHARYAEAA
                     DFRKVHEEDPQAREVVTTALGLEGLKRQWGVHAAGVIMSSEPLADLIPIMRREQDGQI
                     ITQFDYPSCEKLGLVKMDFLGLRNLTILDDAIANVVANRGEAIDLDALRDAPTDEATY
                     ELLGRGDTLGVFQFDSGGYKQLCRQMRPTEFSDIIALGALYRPGPMGTNTHTNYALRK
                     NGQQAVEHIHPELAGPLSQILGETYGLLVYQEQVMAIAQQMAGYTMGGADALRKAMGK
                     KNLAVLEKEYPAFEAGLMRSGYGRDAVKTLWDTLVPFSNYAFNKSHAAAYGLVSYWTA
                     YLKANFPAEYMAALLTSVRDDKDKSALYLNECRHMGIKVLPPDVNSSVGNFAAVGSDI
                     RFGLEAIRNVGRNVVESIIAARTEKGDFTSFEDFLRKCPATVLNKRTIESLVKAGAFD
                     SLGHQRAGLVRIHEAYVDALVDEKRQEAIGQDSLFGGFGFGGDDGEAVQLVVLPPIPE
                     VEWDKATLLAFEREMLGLYVSDHPLFGIEHVLAAHADTSIASLMGDEPRPDGTQVTVA
                     GLVTNLQLKRTKKGDLWAIATVEDLDGAIECLFFPSSYQNVATRLVPDLVVSVRGRVN
                     ARDDTVSIYAQELNVPDLTDGPRGPVVLSMSLRQATGAGIERLRDILGQHPGVTEVHV
                     RLNQPGRSVLMRLDDTYRVTPSPALYGDLKALLGQGCLA"
     gene            31917..>32403
                     /locus_tag="PLB86_01875"
     CDS             31917..>32403
                     /locus_tag="PLB86_01875"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRC63916.1"
                     /translation="MPGRAAYPRQGYAVTPAQRRDLAFRRLAALRLAPLAPVAARTPL
                     AVVEHHLAMQAQDLSSGLWSIGVRTGGTVSDVLASVERGELTRTWPMRGTLHWVATAD
                     AGWLTALLAAPSMRAAARVLEREGVIDEVVERAGRLWTEHLTGAGAMTRAEASAALTA
                     GG"
BASE COUNT         4696 a        11551 c        11200 g         4756 t
ORIGIN      
        1 ctcgacggtg tcgacgactg tggcgaggga ctgccgagcg ggctcgccga ggtagccggc
       61 ccgcgccacg tgggcctggc tgtcgcgcag cgttccacca gcgggagtcg tgatcccgag
      121 gtcgtgcaac tcgctggtga acagctccca gtgcgcctcg atgacctcgt tcggcgggcc
      181 gccgcgccgg gagcggaccc ggcgcagcag cgccgccgtc accggcagca caagcgttgc
      241 gacgacgacc aggccgacga cgagcaggaa gaggtggggg atctgcgcga cccacgagag
      301 cacgcggtcg aggatcggga ggtccaactc ggtccgcgcg gtggtcgtgt cgtccgcgtc
      361 atccggcgca tcccggtcgg gtcgatcgag cgggtcctcg gtggatcggg ccgtcgaggt
      421 cgcgaggggg gtgggcgtca ccgggggaac cgtccacgac ggcgccgtcc cggtccgcac
      481 gctcggcgtc ggttcgaacc gcacccagcc cgccccgggg aagtacagct ccggccagga
      541 gtgcgcgtca ccggccgtga ccgtccagag gccgccgtcc tggctgccgg ggaggaagcc
      601 gatggccacg cgggccggga ttcccgcggc ccgcgacatc atcaccatgg ccgaggtgaa
      661 ctgcacgcaa tagcccgtct tggtctgcag gaacgcggag atcgggtcgc ccacgacccc
      721 gctgaccagc ggagcctccg ggagcgtcag cgagtaggtg aaaccgccct tggaacgcag
      781 ccactcctga atggccacgg cctcgtcgta gggagtggcg gcccctgccg tgacggtcgt
      841 cgtcagcgcg cggacggcgg cctccgagcg cggatcgacc gcgagcgcgg cggacatcgt
      901 ccggttggcg gggaggcggt caggccccgg tctgccgtcg atcccctcgc gcaggaggtc
      961 ggccgtgagc accggctcga ggtaggtggt cgagtaggcg ttcgggcggg tctgcacgga
     1021 gacgtcggag gtcgcgacat cgacctgcca cgagacaccg ctgaggtcgg ccgacaggat
     1081 cggctgcggg gtggccaacg atggcgggtc cagggcgttg ctctccacga cgatcgtgcg
     1141 ttcggatcgg accaccgtgt cggcgaggtc caaccgggca ctgcgaccga gggtgggcgc
     1201 cgggcggctc cagttggtgc cgtcgtatgc cgtggccgcc agcacccgca ggggcgccgc
     1261 tgccggagca ttggtgcggt aggtgaggat cgtgcccgtg cccccggagg tgaggctcaa
     1321 cccgacgtcg agggtggagt tgaacccgac cttgccgaca ccgcggcctg cgccgtcggc
     1381 gctgcgggcc agcccgtcca agaggtaccg agggggaagg tgtgggagca ccgcgggaag
     1441 ggcgatcgcc gccaccagcc cgaccgcgcc gagccgccgg gcgatcgttc cgaagcgaag
     1501 ctctggcgcg gagtcgagcg cagcgccaga gatgggggcc atggggcgcg ccgctgccga
     1561 ggcccacccg cgcatcccca cctgggcctg gcgacccaac atcaacagcc aggcgagcgc
     1621 agcaatgatg aagtaggccg ggggcaacgc cgagccgctg ttggcggcgg cggcgaggaa
     1681 cgcggtgagc aggggcagcc cggccgccgc cggcgccgcc cgggtgacgg cgagggcatc
     1741 gacgaggatg gccacgcccg ctccaatgag ggccagggtg acctcgacgc cgacgttggc
     1801 caggagggga gccgaggact cggcgaccgt ggtgccgaac agcgtgaaca actcggtgaa
     1861 ccgccccacg gtcgaccagg tgggcacgcc ccaccacgtc gtcgaaccgg catactgcca
     1921 ggtcgccatg agcagcacgc ccagcacctg cgcgggcacg atgagccacc cggaccggga
     1981 gacgaaccgt cggatcaacg cgccgatccc gaccgccacg aagacgaacc agatggttcg
     2041 ggcataccag gtctgggtca ccaccagggt ggtcagcccc agcagcgccg cgaggctggc
     2101 cagggccgcg agggccgggt tcgcgccacg gcgggtcatc ggctcgcccc caaggccact
     2161 tgggcatcgc gtcgggagcc gacctggctc cacgcgtgct ccaccgagtc gtcgcgggtc
     2221 accgtggtgg cccgccaacc ggccccgcgc aacagatcga cggcacgcgc ggcctccggg
     2281 gcgaccgcac cgcctcccgc gttggcgccg cctcctgccg ggcgaccggc gaacgatccg
     2341 gtgtcgagga ccatgccgag ggcggtcgct ccgggatggc gcagcgtggc caccgaccgc
     2401 aacgccgact cgtcgtgggc ggcgaggacg gcgacgagca ggccgccccc agagagcagt
     2461 ccgagcgcgg cccgggtgag tcgggccaac gccagcccgg tgtccggctc ggcgaccgca
     2521 aggacggcca acgcctgcgc cgggtcgatc gggagcgcat cgggcccgtc cccgaccgtc
     2581 tcgggggtga gcaggtggac ggcatacccg aggccggaca ggtgcaccac catcgaggcg
     2641 gcggcactca ccgcccactc gaaggaagag ccgatgccga ccccggcgtg ggcgtcgacc
     2701 cgcgggtcga gcaggacgat ggcgcggcgc ctggccggtc gatcctcctg gcgcaccatg
     2761 agttcgcccg tgcgcgcagt ggccggccaa tggatgcgcc gcaggtcgtc gccgtcgcgg
     2821 tattcgcgga tcgactggtc gtcctcgccg tgcaacgcca ccatgaaggg aatctcaccc
     2881 tcggcgccca cccccgtgcc cggcggtcga ttgccgccga gcgggtagac gcggggcagg
     2941 acgaggacct cggtcggctc gccggcctcg acgaatcgct ggctgagccc gaacgggtcg
     3001 cgcagttgca cggtgagcgg cccgagcagg tgacggccgc gcagtggtgg gcggacggca
     3061 tacgtgaggg cgcggcgttc gcccgcgccg agcgagccga gcagcacccg cggtcgttcc
     3121 ccgaggacgt ggtcgagttg ttcctcggcg agcatgagcg gggttgaccg gtgaccgccg
     3181 ttggcgaccg tcgtggtcac cgtggtggcc tcgcccgcgc tgatctgcac ggggtcggag
     3241 gtcctggtga cgaccaaccc cggcgggcgg cgacgggcga gcagaccggc cagcagcggc
     3301 aacccgagca ggagcacgcc gacccgggtg aggtcacgga agccgagcac atagccgcag
     3361 accatgaggg tgatccccgc cgagcagaag gctcgaccgc gggtggtgag tacctcgccg
     3421 aaacgtcgca tcgtcctcgc ctggccgtat gccgcgccgc gcggtcagcg ccgcccgatg
     3481 gggacggggg tggaccggag gatctcggcg agcacctccg ccgggctccg gtgggccaac
     3541 gtggcctcgc cggacagcat gagtcggtgg ccgagaacgg ccggagccac ccgctgcacg
     3601 tcgtcaggca ggacgtggtc acgagcctcc agcgcggcgt gggcgcgcgc ggcccgcagg
     3661 acgtgcagag ccgctcgcgg cgaggcgccc agccgcacgg cgggggaggt gcgcgtcgcg
     3721 ttggcgaggt cgacgacgta ttgccggacg gcgggggagg cgtagacgtc ctgcgtgacg
     3781 aggaccaacc gggccaccgt ctgggcgtcg gtgaccggga cgagggtgtc cagcggcgag
     3841 cgccccccgt gcaggtcgag catctcgacc tcggcggccg tcgacgggta acccatcgag
     3901 atccgcgcca tgaaccggtc gcgctgggcc tcgggcagcg ggtaggtccc ctccatctcg
     3961 atggggttct gggtcgccat gacgatgaac ggccgctgca gcgagtaggt cgtgccgtcg
     4021 accgtgacct ggctctcctc catgcactcc agcagtgccg actgggtctt gggggaggcc
     4081 cggttgatct cgtcgcccac gacgacgttg gcgaagatgg cgcccgggcg gaactcgaag
     4141 tcgcgcaggt cctggttgaa gacgctcacg ccggtgatgt cgctgggcag caggtcgggg
     4201 gtgaactgga tccggcgaac cgagcagtcg atcgaccggg ccaaggcctt ggcgagcatc
     4261 gtcttgccga cgccggggac gtcctcgatg agcaggtgac cgccggcgag caggacggtg
     4321 atcgcggtgc gaaccaccgc cggctttccc tcgatgaccg actccatcgc actggcgatg
     4381 cgcgaggtca cggcgcggag ctccgccagg gcttcagggg tcgtgcgcac cacccccgct
     4441 gcggtcgggt cggtgtcggg tcgggtccgg gcgtcggtca cggttgttcc tccgggtgct
     4501 ctgccgtagc gacggccccg ccgggtgggc ggtgccgtcc tccacttcgc tccaccgggg
     4561 gcctggctgg gcaccccagt ccggcgagca taggtcggct cgctttgaaa tgcggccgaa
     4621 cccgggcatc gggtgagtaa cggaccggtt gcgagggggt ccggcgcggg tcccgtgcgg
     4681 gtcgccgccc tcctgggtat gccgtgcacc gcacccgagc ggctcctcgc ggggtggctg
     4741 cgcggcatac cctccacttc cctccaccct cctgacctgc ggcgttatgg gtcctaccaa
     4801 gaaaatcggg aaatctgggg cgtcaagtgg agggaagtgg agtatcgtgg ggcgccaagg
     4861 agcgtggtgg tgaagtctca tcgcccgccg ggtcgtgtgg gtgggcccct cagggggtct
     4921 gggagggaag gggtggtgcg ccgtggtcga cgtccccatc gccccgctgc cgaccccgtt
     4981 cctcggcacg cacaccccga agctggacga caaagggcgg ctgatcctgc cggcgaagtt
     5041 ccgcgagccg ctggccgagg gactggttat ggccaagggc caggagcgct gcatcgtcgt
     5101 ctacccgatg gccgagttct accggttcgc cgcgcagatg cagacggcac cgagttcggt
     5161 gtccggggtc cgcgactaca cgcgcgtgct cgcctccagc gcctcgccgg aggtccccga
     5221 caagcagggc cggatcacca tcccgccgat gctgcgcgag tatgccgcgc tcggccggga
     5281 cctggccgtc atcggcaaca tcagccgcgt cgagatctgg cagttggacg cctggaaccg
     5341 ctacctggcc gacaaggagc cgggctatgc ctcgcagagc gaagaggtga tgcccgggag
     5401 gttctgaccg gctgaccacc tgcgcccagg tctccagccg cttccgtccc gacacgactt
     5461 cccccgtggc gggccgacgc ggttcgagaa ccatcccgaa gcggatgggg acctgagcgc
     5521 agggccatat cggccccgac ccacctatcc gtacccaccc cacctgtccc caccccatcc
     5581 gtccctcacc cgtccccgcc acgtcgaacc acctcccagc cccaccccgt cccactgagc
     5641 caccagacct cggagttcgc gttgaccacc tcgccagagc gcgccacatc ggaccggcat
     5701 gtcccggtcc tgcgcgaccg ggtgctggag ttgctcgcgc ccgcgctggc cgcagagggc
     5761 gccgtctatg tcgacgggac cctcgggatg ggtgggcacg ccgagggcgt cctgcaggcc
     5821 ttcccccacg cgcgactcat cggcatcgac cgcgacccgc aggcgttggc tctcgccggg
     5881 caacggttgg cccccttcgc cgaccggatc accctcgtcc acgcggtcta cgacgagttc
     5941 gcccaggtcg tcgacgcgtt gggcatcccg acggtggacg cggcgctgtt cgacctcggg
     6001 gtcagctcat tgcacctgga cgagaccgac cgtgggttcg cctaccgcgt cgacgcgccg
     6061 ctggacatgc ggatggacca gacccgcggc atgaccgccg ccgacgtcct caacggctac
     6121 gagccggccg acctcgagcg gattctgcgc gacttcggcg aggagcgcta cgcccgctcg
     6181 atcgtccgcg cgatcgtcgc cgaccgcgcc aaggagcctt acacgacgtc cgggcccctg
     6241 gtcgacatgc tccgccgggt cgtgccgggg gcgtcgcagc gcagcggcgg ccacccagcc
     6301 aaacggacct tccaggcgct gcgcatcgag gtcaacggcg agttggacgc ctggcgccga
     6361 gccctcccgg ccgctgtcga tcgcctgcgc gtgggtggtc gagtcgccgt cctctcctac
     6421 cactcgctcg aagaccggat caccaagcgc tgcctcgccg aaggcgcgac ctcgcgcaca
     6481 ccgatcggta tgccggtcga gcggccgcag gacgcggcat acctcaagct cctcacccgc
     6541 ggcgccgagg tgcccaccga cgcggagatc cacgacaacc cgcgcgcagc ctcggctcgt
     6601 ctccgcgccg ccgaacggac ccgtccgacc acccaggggg aacaccgatg agtcaggcca
     6661 gtgcgcttcg acacgccgcc agcaccagcg gccggccagc ggggcgaccc agcggtccgg
     6721 gcgcccgcct gcgggtcgtg cctgccgcca tcgagcggac cggcaccggg aagttcgcgg
     6781 cgatctgcat ggtcctgctc accgtcggcc tcctcgcgct gctgctgctc aacacccagc
     6841 tcgcccaggg gtccttccgg ctgcacgatc tccaggtcgc ctctggcacc ctgaccgacc
     6901 aggagcacga gctcactcgc gctctcgacg ccgaacgcaa ccccgggtcg ctggccaagc
     6961 gcgccctcga catgggtatg gtccccgcga cgtcgatggc cttcatccgg ctcgaggacg
     7021 gggtcatcct cggggtcgcc gagccggcgg cagccaaggt gctcaccgtc gtcaacgcgc
     7081 cgaagactca acccagcgct cccgcccccg tcgccccgga tccggccgcg acccctgcgc
     7141 cggccgcgac cccggcgccg accgcgaccc cggctcctgc cgatgccgcg gcgtccgatc
     7201 ccgaggtccc cgacggcgcc cccgtgaccg ccacggttcc ggcggggtga cgacatgacg
     7261 ttgccgcccc gtcagggtca accccccacc aatagacgcc gcccgcccga ccccagccca
     7321 caccgcgtgc ccgaccccag cgcgcgccgc gtgcccgacc ccacagcccg gccgcgcgcg
     7381 ccgcgcgcca cggcaccgac gcggcggcct gccgcgcccc cacgtccgcc gcgacccgcg
     7441 cgtccgcggc ggatccgtcg gcacctgagc gtcgggcaca cgacgtggcg cgtgcgcacc
     7501 atgtgggtcg ctgtcctctt cgtcctgtcg atcttcggcg cccaactcgt gcgggtgcag
     7561 gcgttcgacg cctccggcgt gcaactggcc gccctcaaca agcggctcaa ctcggtggtc
     7621 gtccctgcgc tgcgcggtcg tatcgtcgac accaagggga ccgtcctggc cgcgagcgtc
     7681 gagcggcgca cgatcaccgt caaccagaac gccgtcgcca cctacgagcg ggtcatcgag
     7741 gggaagcggg tcaaggtggg cgtcgtcggc gcggccgagc gcctggcacc cctgttgggg
     7801 atgacggccg acgaactcgt caagaagctc atgggcacca gcggctatca ggtgatcgcc
     7861 aagggcgtgt caccactggt gtggcgggag atcagcgcca tggggatccc cgggatcctc
     7921 agcgaggtga ccttcgagcg ggagtacccc tcggggatga cgggcgcgcc catcgtcggc
     7981 ttcctcggcg atgacggcag cgtggccggt ggcgtcgagc agatgatgca ggcccagctt
     8041 gccggcacgc cgggcaagga gatctacgag caggcgcgcg acggcaaggt cattccatgg
     8101 gcccaacggc tctcgcagcc tgccgtcgac ggccaggatg tgcgactcac catcgacgcc
     8161 gacatccaat ggttcgccca gaacaccatc ggcaacctcg tgcacgcgat gggtgccaag
     8221 tcgggcacca tcgtcgtgat ggaggccaag accggcaagt tgcgggccgt tgcgccctac
     8281 ccgagtttcg atccgaccga cctggcccac accaaggcat ccgcgctagg caacaaggcc
     8341 ttccaggagg tctatgagcc ggggtcgacg gcgaaggtca tcaccatcgc ggccgcgttg
     8401 gaagagaacg tcgtcgagcc gatgacgggg gtcatcgtcc ccaaccggct gcggcgtgcg
     8461 gacacgacct tcaaggacgc ccacgatcac ccgaccctga acctcaccgt ggccggggtg
     8521 ctcgcgaagt cgagcaacat cggcacgatt ctggcgacgg aaagtatgcc ggccgccacc
     8581 ctcgaggcct accagcgctc gttcgggctg gggaccaaga gcgggatcgg ctaccccgcc
     8641 gagtcggccg gcctgttgcg tcccgccgcg atccagaccg ggtcggagcg ctacacgacg
     8701 atgttcggcc agggcatgtc cgtcaccgct gtccaggcag ccggcgtgtt tcagaccatc
     8761 gccaacggcg gcgtccgtgt gccgccgagc ctcatcgaag gcacggtcgc cgaggacgga
     8821 accttcgcgg cgagcacggc cccgcaaggg gtgcgcgcgg tgtcccaaca gacgtccacc
     8881 gaggtgagcc aaatgctcga ggaggtcgtc ggaccgggcg gcaccgcccg gcacgtggcc
     8941 atcccgggtt accggatcgc cggcaagacg ggaacggcca accgggtcgc cgacagcggc
     9001 gccggctaca gcggctacac cacctccttc atcggctacg caccggccga ggacccgcag
     9061 ttcgtcgtcg cggtcaccct ccagaacccc accgccggcg ttcccagcgg tggcagccag
     9121 tgcgggcccg cgttcaagga cgtcatgacc tacgtgctgc aggcctacca ggtgcctccg
     9181 acgggtgcgg ccagcgccga ggtccccctc gtccccgctg agcccctcgt gcctggcgca
     9241 cccggcgtga tcagcgaccg caagtccacc ggctagcctg atcgccgtgc attcccaccg
     9301 gccccgatcg cttccgccgg tcgcccttga gacgctcgcc gaccgtgcgg gcgccacggt
     9361 cgtcggcggg gggagcgcgc agggggtcct cgtcaccggg gtgagtctcg actcgcgcac
     9421 cgtccgaccc ggcgacctgt atgccgccct tccgggcgcc cacgcgcatg gcgccacctt
     9481 cgtggcgggc gcggttgcgg cgggcgccgt cgccgttctt accgacccgg agggggcgcc
     9541 gctggcagcc attggccttg gcgtcccgat cctgctcgtc gaggcgccgc gcgcggtcct
     9601 cggcgaccta gccgcactgg tctatggcca tcccgcggac gcgatgcggc tgatcggcgt
     9661 caccggcacg aacggcaaaa cgaccaccgc gtatctcatc gagtcggcgt tgcggggtct
     9721 cggagcccgg actggactca tcggcacggt cgagacccgg gtcggtgacg aacggttgac
     9781 ctctcgtcgc accactcctg aggcacccga cctgcacgcg atcctcgccg tcatgcgtga
     9841 ggcggggacc gactcggtcg tcatggaggt gtccagccat gcgttggccc agcaccgggt
     9901 cgacggggtc gtctatgacg tcgcactctt caccaacctg tcccaggacc acctcgacta
     9961 ccacgcgggg atggccgact acttcgcagc caaggcggcg ttgttctccc cggagcgcgc
    10021 gcagcgcggg gtcgtctgca tcgacgacga gtggggcgtg gcgctcgcgc aacgacgcgg
    10081 catacccctg gtcaccgtct cctcgcgtcg accggccgac tggacgctca cggcggggga
    10141 ggggccggag gtgacgctcg tcggccccgg tgagcagcag ttgctcctgc actgtcacct
    10201 ccccgggacg ttcaacctcg tcaacaccgc catggccgct gtcaccctgc tgcaactggg
    10261 gtatgccgcg tccgaggtcg agcgcgcgat ggcgttggaa cccgtcgtcc cgggccggat
    10321 ggaacgggtc ccgggtgggc ccgacgaccc gcggtgcgtc gtggactatg cccacacccc
    10381 cgaggccgtc gacgcggccc tggccgccct tcgccccagc acggcgggcc ggctcgttgt
    10441 ggtgctcggc gccggcggtg accgagacac cggcaagcgg gcggccatgg gggctgccgg
    10501 agcgcggtgg gccgacgacc tcatcgtcac cgacgacaac ccccgctcgg aggatcccgc
    10561 cgtcatccgc gaccaggtcc tcgcgggggc ccgctcggtc cttcccgccg accctgatgg
    10621 caccaccagt gcccgaagcc tgttcagctc cgacgccggc cgggccgccc acatcgagcg
    10681 cgccgtgagg ctcgcccgcg agcagggtcg ccccgtcgac aacaccgtgg tcgtcctcgg
    10741 caagggccat gagaccggac aggacatcgg cggcaccgtg catccctttg atgaccgtga
    10801 cgcgcttgcc gccgccctgc gcggagcgcc ctaccgccca gagggcaatg acagtgggca
    10861 cgtgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaccg agggcgagct
    10981 atgacggaag gcaatcgatg atcccgctgc gcctggacca cctcgcgcgg gccgccggtg
    11041 ccgtcctgat ggacgcgcac ggccgccctg cgggccccga cgcgcccgag gggcgggtgc
    11101 tcgttgacgg ccccgtcgtc accgactctc gtgaatgtcg gcctggcagc ctctatgtcg
    11161 cccgcgtcgg cgagcaggcc gacgggcacg ccttcatcgg cgccgcccag gaagccggtg
    11221 cggtcgccgc gctgacctcg cgcccggtcg gcggggtcgc ccacctgctc accgacgacg
    11281 tacagacggc cttcggcgcc atcgcacggg ccgtcgtcga cgctgccccc gacctcacca
    11341 tcgtcgggat cacggggtcg tccgggaaga cctcgaccaa ggacctgctc gcccaggtcc
    11401 tgcgcaccgg cgccgagacc gtggcccccg aaggctccta caacggcgag atcggcgtcc
    11461 cgctgacggt ctgccgaatc accccgacga cccgcttcct cgtcgccgag atggggtccc
    11521 gggggatcgg ccacatcgcc tacctcaccc ggatcgcgcc accgcgcatc gggatcgtcc
    11581 tcaacgtggg gcaggcgcac gtcggcgagt tcggtggccg ggacaacatc gcccgcgcca
    11641 aggccgagct cgtcgcggcc ctaccggcca gcggtgtcgc catcctcaac gccgacgacc
    11701 ctgcggttgc cgccatggcc acggccacca cagcccgggt cgttcttgtc ggtgagagcg
    11761 ccgccgccaa catccgcgcc gaagaggtcg aactcgacga gtccggccga ccgagctttg
    11821 gggtgctcag cccggccggg acggctcagg tgcggttgcg tctgcacggg cgtcaccacg
    11881 tcggcaacgc cctgagcgtc atcgccgccg ccctcgaatg cggcctgccg ctcgacgaca
    11941 tcgtcgccgc cctcgcggtg gccggtccgg tgagccgctg gcgcatggag gtcaccgagc
    12001 gggccgacgg cgtcaccatc gtcaacgacg cctacaacgc caaccccgac tcgatgaggg
    12061 ccgcgctgcg tgcgctgcgg gccatgaagg gcccggccga cgccggaccc gggcggcgga
    12121 cgtgggcggt gctgggggag atgttcgagc tcggcccgga ctcccacacc gaacatcagc
    12181 aggtcggtgc ggtcgctgtg gcccaggacg tcgaccggct ggtcgtcgtg ggcgcgggcg
    12241 ccggcctcat tggtgatggt gccgtggcag cgggtatgcc gcccgcgcac ctcgcgcgcg
    12301 tgcccgacac cgacgcggca tacgccctgc tgaccgagaa tctccgccga ggtgacgtcg
    12361 tgttgttcaa gtccagtcgc gacgcgggac tacggtggct cggtgaccgc gtcgccgaga
    12421 cggtccacgg gaacgaggtc aggtcgtgaa gggcgttctg gtggcggctg ccacctcgct
    12481 cgtgctcgct ctgctgggca cgccgtggtt catcaagtgg ttggtgaagc ggcagtacgg
    12541 ccagttcatc cgcgatgacg ggcccacttc gcaccgcacc aagcgcggca ccccgacgat
    12601 gggcggcacc gtcattgtcg gagccaccct cgccgcctac ttcctcgccc acctcgtgct
    12661 gggcacctgg ccgtccgtca gcgggctgct cgttctgttc ctcatgaccg ggctcgcgct
    12721 ggtcggcttc ctcgacgact acatcaagat ctcccggcaa cgcagcctgg ggctgcgcac
    12781 caagcagaag ctgttcggcc agggtctggt cggggtgacc ttcgcggtgc tcgccctgca
    12841 gttcgagaac gccggctatc gcaccccggc ctcgacggcg atctcgttcg tccgcgacac
    12901 gcgtttcgac ctcgcgttcg ctggtccggc gatcgggctg gtgctcttcg tcctctgggc
    12961 caacttcatc atcacggcga tgtccaacgg cgcgaacctc accgacggcc tggacgggct
    13021 ggcgaccggt gcctcgacga tgatcttcgg cgcctacctg ctcatcggga tctggacgag
    13081 caaccagaac tgtcagacca accccggcct caagtgctac gaggtgcgcg acagcctcga
    13141 cctggcgatt gtctcggcag cggcgatggg cgcgtgtttc gggttcctct ggtggaacgc
    13201 ctcgcccgcc aagatcttca tgggtgacac cgggtcgctc ggcctcggcg ggctcatcgc
    13261 cggcctggcc atcacgaccc gcaccgagtt cctctcggtc atccttgccg gactgttcgt
    13321 cctggagacg ctgtcggtgc tcatccaggt gagcgtcttc aaagcgaccc gaaaacgctt
    13381 cttccggatg gcgcccctcc accaccactt cgagatgctc gggtgggagc agatcacggt
    13441 ggtcatccgc ttctggatca tcgccgggtt gttcgccgca ctcggcctgg gcatcttcta
    13501 cgccgaatgg gtggtgggct cgacatgagc gagctgcagg acttcgctga tccgatcgac
    13561 gacggttatg agccgcgccc gggcctcacc caccgcgacg ccgactggtc gggtctgcgg
    13621 gtgctcgtcg caggcctggg gatcagcggc ttcgccgccg ccgatgcgct gtgggagcgc
    13681 ggggccgtcg tcaccgtcgt cgatgccagt gcgccgggag catcccgaac ggtcgacgag
    13741 cgcgcccaga tcctcgccgt cctcggcgtc gacgtccggc tcgggccgga acacaccgcc
    13801 ggtatgccgt ctgatcgggc catcgatctc gtcgtcacct cgccgggctg gcgccccacc
    13861 cacccggtgt tggccgatgc ggccgcgcac ggcataccca tctgggggga ggtcgagctc
    13921 gcgtggcgga tgcgggcccg cgaaggggcg gcgccctggc tgaccgtgac cggcaccaac
    13981 ggcaagacga ccacggtcaa catgctggcc agcatcctgc gggccgcggg cctgcgggcc
    14041 accagcgccg gcaacgtcgg cacccccttg cttgaggctg tgctgcaccc gcagccctat
    14101 gacgtcatcg ccgtcgagtt gtcgagcttc caactgttct ggcagcgctc gctgtctccg
    14161 gtggcgtcgg cttgtctcaa cgtcgcggcc gaccacatcg actggcacgg gtcgttcgag
    14221 gactacctgc gggccaaggg ccgggtcttt cgcaacaccc acctcgcctg catctacaac
    14281 gtcgccgacc ccttgaccga gcagttggtc atcgacgccg acgtcatcga agggtgccga
    14341 gcggtcgggt tcacgttggg cattccggcg ctgtcgatgg tcgggttggt cgacgagatc
    14401 ctggccgacc gggccttcgt cgagcagcgc caccactcgg cggccgagtt ggccacgatc
    14461 gccgacgtcc agggcgaggc cccctcgatc gccccgcact atctggccaa tgccctggcg
    14521 gcggcggccc tcgcgcgcgc ctacggtgtc ggtccgctgt cggtgcgtga cggtctgcgg
    14581 gcgttccgcc ccgaccccca ccggatcgcc gaggtcggca ccgtcgacgg ggtccgctac
    14641 gtcaacgact ccaaggcgac caacccgcac gccgccgagg ctgcattggc gtcgttcgac
    14701 agcgtcgtct gggtcgccgg cgggctgctc aagggcgccg acattgacgc cctcgtcgag
    14761 aaggtcgccg gcaaactgcg cggggtcgtc ctcatcggcg ccgatcgggc actcatcgcc
    14821 caggctctgg cccgacacgc gccggatgtc cccgtcgtcg acgccggcgg gccggacact
    14881 ggggggatgc agaccacggg ggaagacgtc gaccgagtga tggaccgggt cgtcgcgcac
    14941 gcggcgacgc tggcccgccc cggtgatgtc gtgctgctcg cgccggcggc ggcgtcgatg
    15001 gacatgttcg ccaactacgg ggagcgcggc agcgccttcg aacgcgccgt ggggcggttc
    15061 ggcgagcggc cgggtcaacg ctcgtgacca gcaccgtcga tcgttccgcg cgcgcgcccc
    15121 gggcgccccg ggcgacccgg ccgggcgaca ccggtgccgc acactcctgg ttgccggcca
    15181 gcctgcggcc cctcgccgcc cgtttcgaca gcccggtgac ctcctactac gtcctcgtcg
    15241 gggtcaccac agcgctgctg ctcatcgggt tggtcatggt cctgtcggcg tcgatgatca
    15301 cctcctacaa ggacgacggc tcgtcgtatg ccgtgttcct caaccaggcg cgcttcgccg
    15361 gcatcgggtt ggtcggagcg ttcatcgcct cgcggctcac cgtcaagtgg tggaagcgtc
    15421 tggccgtgcc ctccctcgcc gccgccatgg tgctgcaggc gttggtcttc gtccccggtc
    15481 ttgggctctc taagggtggc aaccgcaact ggctcaagct cacctcgacg ctgtcgttgc
    15541 agccttccga gctcaccaag atcggcctgg tcctcgtggg ggcgctcatc ctcgccaaca
    15601 agcgggcctc gttgggccgg gtctcgcacg ccgtcattcc ctatctcgtg ccggtcgccg
    15661 ccatcacgat cggcctggtc ctcgccggtc acgacctcgg gaccgcgctg gttctcatcg
    15721 ggatcgtcgg agcggtgatg tatgccgcgg gcgtgccggg ccggttgttc gcggcggcgg
    15781 cggtgggggc ggcgctgctg gcgacggctc tcgtcgtcac gagcagcaac cggatggacc
    15841 gaatcggcaa ctggctgtcc gggtcgtgca ccgaccccaa cggcgagtgc ggtcagtccg
    15901 tgcatggcct ctatgcgctc gccgacggtg gctggtgggg agtggggctg ggcgccagca
    15961 aggagaagtg gtcgtggctg ccggaggccc acaacgactt catcttcgcc atcatcggcg
    16021 aggagttggg gctgccaggc accttcgtca tcctcgccct cttcgccttg cttgcatcgg
    16081 cctgctaccg catcgtcacc cgctcgaccg atcagttcgt ccggatcgcc gcggccgggg
    16141 cgatggcctg gatcctcttc caggcgatga tcaacatcgg cgcggtcatc gggttgctcc
    16201 cggtcatcgg ggtgccgctg ccattcgttt cagcgggcgg ctcggccttg gtgacgaccc
    16261 tcgtcgcgat gggcatgctc gtgtcgttcg cccgggccga gcccggcgcc gccgagatcc
    16321 tcgctgcgcg tcctggggtg atccagcggt ccctggccgt cctgccccgc cgccgcgcgc
    16381 gctgacccca acccggagtt gccacgatga cccccttcac tcgtaatcgc cgtgcccgcc
    16441 ccgccgactc cggcggcgag ttggtctcgg tcctgctcgc cggcggcggc accgcggggc
    16501 atgtgtcacc gctgctggcc acggccgacg cgttgcgccg ccggcgcccc gacctcaccg
    16561 tgacggcgct gggcacccag accgggcttg aggcgcggat cgtccccgcg aggggctacg
    16621 acctgcggtt cgtcccgcgg gtgccgctgc cccgcaaacc cgacgccaac ctcgtgcgct
    16681 tcccgggtgc cctgcgcgca gcggtcgagg ccgccggacg ggccatcgac gagaccggcg
    16741 cccaggtggt cgtcggcttc ggcgggtacg tctcctcgcc cgcttacctc gcggcccggc
    16801 gccgcggcat acccatcgtc gttcacgagc agaacgcccg ccctgggttc gccaaccggt
    16861 tgggggcgcg gctgactccc tacgtcgcca cgaccttcgc cgccacccac ctggcgcacg
    16921 caacctgcat cggtatgccg ctgcgccgcg agatctccac cctcgaccgg gccgccctgc
    16981 gcgccgacgc acgggccgag ctggggctcg atgccgaccg caccacgctg ctggtgaccg
    17041 gcgggtcgct cggcgccgcc cgcctcaacg agacgatggc cgcccgcagc cgcgatatca
    17101 ccgacgcggg gatccaggtc ttccacgcgg cgggcaacgg caaagggttc gtgccggacg
    17161 tcagtccgtc gggcgcgccc tacatcgtgc gggagtacgt cgaccggatg gacctcgcat
    17221 atgccgccgc cgacctcgtc ctgtgccggt cgggagcgag cacggtgtgc gagctcaccg
    17281 cggtggggct gcccgccgtc tacgtcccgc tgcccttcgg caacggtgag cagcgggtca
    17341 acgccgccga cgtcgtcgcc gccgggggag ggctggtcgt cgaggacgcg gcgctgaccc
    17401 ccgtctgggt cgatgtcgtc ctgttgccgc tgctcaccga cgccgaccgc atcgcgcgga
    17461 tgggctccgc tgcggcgacc gtcggtcgcc gcgatgccga cgaagcgctc gtcgatctcg
    17521 tgctggccgc cttcgcgggg gatcgatgac gctgtcgtcc gggttgccgc cgacccgctt
    17581 cgactatgcc gccccggtgc cgcccatcgc ggagctcggg gctgttcatc tgatcggcat
    17641 cggcggcgcc ggcatgtcgg cgatcgcgcg gatgctgctg gcgcgcggtc tcctcgtcac
    17701 cgggtccgac tcgcgtgaat cggcgtcggt ggatgcactg acccgcgacg ggatcggcat
    17761 ccgcatcggc caggacgcgg cgaacgtcac cggcgttcag accgtcgtgg tgtcttccgc
    17821 gatccgtgag gacaacccgg agttggctgc ggctcgcgcc gccgggctgc gggtcatgca
    17881 tcggtcgcag gcgctcgcgg cgctcgccgc cgactcgccg gtgcggatcg ccatcgcggg
    17941 ggccaatggc aagacaacca cctcgtcgat gctcgccgtc gtcctcacgc tggccggacg
    18001 cgacccgtcg tatgccgtcg ggggagaccc cgtgcagttg gacgccaacg cccgggccgg
    18061 caccggtgag gagttcgtca tcgaggccga cgagagcgac ggctccttcg tcggctatca
    18121 ccctcacgtc gctctcgtga ccagcgtgca gcccgaccac ctcgacttct acggcaccgc
    18181 cgaggcggtg gaggcgggct atcgggcctt cgtcgacacc cttgacccag gcggcctgct
    18241 cgtcgcctgc ctcgacgacc cgggagcccg acggttggcc gacctggccc gcagccaggg
    18301 ccgccgcgtc gtcggctacg gcttcagcgc cgatgcgcag gtccggctat ccgatgcccg
    18361 gttggggggc ctcggggccg aggcgaccat cgaagtcgac gacgcggcat acccgatgcg
    18421 gttgtccgtg cccggcgagc acaacctcct caacgccgcc ggggtgttgg ccgtggcgct
    18481 cttcgccgtc gtcgcgagcg agccgcacgg ggctgggcgc agcccggagc gagtgatcgg
    18541 ttggctagac ctcttgtcga ggtttagtgg ggttcgccgc cgttttgagc gcgtggggga
    18601 ggctgtcggc gttgtggtcg tcgacgacta cgcccacaac ccggccaagg tccgggcggc
    18661 cgtcgccacc gggcgcggtg cggtcgaggc cggggggcgg ctcgtcgtcg tcttccagcc
    18721 gcacctctac tcccgcacgc gagacttcgc cgccgagttc ggagcggcgc tcgcaccggc
    18781 cgacgtcgtc gtcgtcaccg atgtgtatgc cgcccgagaa gaccccctgc cgggcgtcac
    18841 cggcgcgctg gtcgccgacg ccgtgcgggg ggttcgtgcg ggcgtccagg tggagttcgt
    18901 ccctgctctg ggcgatgtcg tcccagccct gaccgggctg gtcgctccgg gcgacctcgt
    18961 cctcacggtg ggtgccggcg acatcacctc gatcgggcca gccctcctcc tggcgctggg
    19021 cgcccggtcg atcagggagg gccagtgagg gacaccctga ggtcgtgggt gagggccgtc
    19081 cagcgacggg tccgaggcga cggtggtccc cggcggttgc cgtcggcacg agtgcgtttc
    19141 gaacagcggg cccacgccgc gcgccgccga ccctgggtgc tcgtcggaac cgcggtggcg
    19201 atcctggcgc tcatgggcgg cctgggctgg gtggtctggg ccagtccggt gctcgtcgtc
    19261 gacgaggtcg tcgtcacggg ccttgacggt gccgaccgtg atgccgcgct gactgcggct
    19321 gcggtcccga tcggtgagcc cttggcccgg gtcgattccg cggcagtcgc ccagcgggtc
    19381 ggcagcctcc cgatcgtcaa gcgggcggtc gtcacccgca gttggccgcg caccctcgtg
    19441 gtctcggtga ccgcgcgtgt cgggatgctc gtcgtgcgca actccgaggg caccttgcag
    19501 gtcgttgacg aggacggggt ggccttccgc gatgtcgacc aagcaccggc cgggttggcc
    19561 gtcgtcaacg gcagcgggca gcggccggca ccggaaggcc tgcaggcggt gatcgaggtg
    19621 ctgcggatcc tgccggccga ccagcgcgcc acggtctcgg cgatcacggt atcgagcgcg
    19681 agcatggtga ccttcgccct cggcgaggtg gaggtcgtgt ggggcggccg tgggtcggag
    19741 gagaagaagg ggaaggtcct tcaggtgctc ctcaagacca cccctgcgcc gaaggtcatc
    19801 gacgtcagcg ctcctgacac cccggtgacg cgctagtagt cacaacggtc acatcagtca
    19861 cacaagcctc agaaatccca cgagtcgcgg ggatataccc gaatcttgac gttaggtcca
    19921 agacacgccg agtgggtcgt tggaaaccct cggtctagtg cctacggtcg gtgcgacggt
    19981 agagaccgag aaacactgta tctcgactag accttcaggt tcggcagcgg ttctccggcg
    20041 tgtcggacgg ggcggttctc cgcctcgacc gcaggccgta gtgccggggt cgggcccgat
    20101 cggacgacac gaatcgcgcc agaacgcaac gaaaggccac caccgtgggt gcagcaccgc
    20161 agaactacct cgccgtcatc aaagtcgtcg gcatcggtgg tggcggcgtc aacgccatca
    20221 accggatgat cgacgtcggc ctcaagggcg tggagttcat cgccatcaac accgacgccc
    20281 aggcgttgct gatgagcgat gccgacgtga agctcgacgt cggccgcgag ctgacccgcg
    20341 ggctcggcgc tggcgccgac cccaacgtcg ggcgcaaggc ggccgaggac cacgccgagg
    20401 agatcgaaga ggtcctgcgc ggagccgaca tggtcttcgt caccgccggc gagggtggcg
    20461 gcacgggcac cggtggcgcg ccggtggtcg cccgcatcgc ccggtccttg ggcgccctca
    20521 ccatcggtgt cgtcacgcgg ccgttcacct tcgaggggcg tcgtcgggcc aaccaggccg
    20581 agtcggggat cggcgggctg cgcgaagagg tcgacaccct catcgtcatc cccaacgacc
    20641 ggctgctgtc gatcagcgac cgcaacgtct cgatgatgga cgccttccgc agcgccgacc
    20701 aggtgctcct ctccggcgtc cagggcatca ccgacctcat caccacgccg ggtctgatca
    20761 acctcgactt cgccgacgtc aagtcggtca tgcagggcgc cggctcggcg ctcatgggca
    20821 tcgggtcggc gcgcggcgag gaccgggccg tccaggctgc cgagctcgcc atctcctccc
    20881 cgctgctgga ggcgagcatc gacggcgccc acggtgtgct cttgtcgatc tctggtgggt
    20941 ctgaccttgg gctgttcgag atcaatgagg ccgcccgcct ggtccaggaa gccgcccacc
    21001 ccgaggccaa catcatcttc ggaaccgtca tcgacgacgc cctgggcgac gaggtgcgcg
    21061 tcaccgtcat cgccgccggc ttcgacggcg gcgacccgac accgcgctcg gacgcccgcg
    21121 cgctcggcca ggtgcaggcc ggtggcccag gccgcccggc cgcggcttcg ggcccgagca
    21181 cggctgcggc cggtggctcg tcggcgggcg cggcatacgc ctcaggtccg gcggcaaccc
    21241 cggctccggc gcctgccccg gctccggcac ctgccccggt cgtcgtgccc cccgtccagg
    21301 tgcccccgcg tccggtgagt gtcgacgacg cggacgacct ggacgtgccc gacttcctca
    21361 agtaaccgca gcgcgtcggt ggtcgacacg tccgtggtcg tcagcgtccc gcgttagcgt
    21421 ggagccgtgt tcttctggca cgaacggcgc gccggtatcg acgtggccat caccgaccgc
    21481 cacggcgggg tgagctcggc acccttcgga gagctcaacc tggcccgtca cgtcggtgac
    21541 gaaccggccg cagtcgaggc gaaccgcgcg gcggtggccg ccgagcttgg tctgcccgct
    21601 gagcgactgg tgttcatgga ccagtgccac ggcgccacgg ttgaggtcat cgacggtccg
    21661 tggcagggcg atccgcccgc gtgcgacgcg gtgatcacgc gggccagtga cctggcgctg
    21721 gtcgtcatgg tggccgactg cgtccccgtc ctgctggctg accccgaggc cggtgtcgtc
    21781 ggcgccgtcc acgccgggcg tccaggtctg gtcgccggtg tcgtcggcgc cgccctcgac
    21841 gccatggccg accttggcgc gcgcgccccc tacgccgttg tcggtccgtc ggtgtgcggt
    21901 cgctgctacg aggtccccga ggagatgcgg gcggcggcag ccgcggtcga gccggccagc
    21961 gccaccgtct cgtggaccgg cacgcccgct atcgacgtcg gcgccggagt cgtcgcgcag
    22021 ttggccggtc gcggcgttcc ggtgacgtgg gtgccggggt gcacccgcga ggacgaccgg
    22081 ttgttctcct accgccgcac ctcaccgacc gggcgcttcg ccggggcgat ccgcaggtat
    22141 gccgtggacc cggcatacct gatcgagcga cccagccacc ccacggagac gtcgtgaccg
    22201 ggttttcgcc ccagccgacc gaggtgggcg accagcgggc cgaggcgttg cgggctgggc
    22261 tcgccgacgt gcgggagcgg gttgctcgcg cctgtgacgc ggccgggcgc gatccggccg
    22321 aggtcaccgt gatcgtggtg acgaagttct tccccgcctc cgacgtgcgt cggttggccg
    22381 ccttgggggt gcgggacatg ggggagaacc gcgaccagga ggcgtccgcg aagttcgccg
    22441 acgtcaaggc cgacacggca ggtctgcggc tgcacttcat cggccagatc cagaccaaca
    22501 aggcggcctc ggtcgcgagc tatgccgatg tcgtccatac cgtcgaccgg ctcaaactcg
    22561 tcacggcctt ggatcgaggg gctgccgcag ccgatcgacg gctgcgcgtc ctcgttcagg
    22621 tcgacctcga cccggcccgc cccgcggcga cgttaggagc ggtggcgact gcggcggcgt
    22681 cagcgggacc ggctagggga ggcgccgacc cggcggacgt ggggggcctg gtcgccgcgg
    22741 tgcaggccag cgccgctctc gaccttctcg gtgtcatggc ggtcgcgccc ttgggcgtcg
    22801 atcctggccc cgcgttcggc aggctcgccg acgtctcccg cgcggtgcgc gcggtcgttc
    22861 ccgacgccac ctggatctcc gccgggatga gcggagatct tgaggtggcg atccgtcatg
    22921 gcgcgacaca cctgcgtgtc ggcacggcaa tcctcggatc gcgcccgacc cttcgctaac
    22981 gtcgagccag gcccgtatgc cgcgcacccg ctgcgactgc tggctgagta gacatccacg
    23041 gataaagggg atcggcccat ggctggggca cttcgcaaga cgatggtgta cctcgggctc
    23101 gccgaggagg acgagcgtta cggctcctac gacgaggcct acgaggacga ccgcgtctcg
    23161 gagcacacgg ctgccgtcac gcccttgccg tcccggccga cctcggtcgc gtcggtcgtg
    23221 cgggaccccg acatggggtc gctcacccgg atcacgacga tccacccgcg cacttacaac
    23281 gaggctaagg cgatcggcga gtcgttccgc gagggcaccc cggtcatcat gaacctcacc
    23341 gagatggacg acgccgacgc caagcgcctc gtcgacttcg cggccggcct ggtcttcggc
    23401 ctgcacggcg ccatcgaacg ggtcaccgcg aaggtcttcc tgctctcgcc ggcccacgtc
    23461 gaggtcaccg ccgaggagtc tccggcggtc cgttcgccgc ggagcccgtt caaccagagc
    23521 tgagcggccg ggccgagtcg gcgcggcggc atacccacct cacagccaga tcggggacga
    23581 tgggccgatg cgcggcgtgt ggcaggtcat ctcgaccctt ctgttcatct tcttcgtctt
    23641 cctcatcggt cgactcgtgc tcgactgggt ccgggtgctg gcccggtcct ggcgacccca
    23701 cgggatcgtc ctggtcctgg ccgaggccgt ctacaccgtc accgatccgc ccctgcggtt
    23761 ggtccgcaag atcgtcccca gcatcacctt ggggtcgatc cgcatcgatc tggccttcat
    23821 cattctcttc ctgctcacgt ccaccctgat gagcctgcaa ccgttctagc gcaggcgaat
    23881 tggggacttt gagcagacgc aaccgcgccc gaggatcggg cccttacgcg acgtcggcta
    23941 ccgtccggtt gggtcatcag gactcgatta gataacgtta ggtcgctact atccactgtc
    24001 cgtcgaggtg aaccatggcg ctcacgcccg aacaagtgct gaacaagcac ttccagacga
    24061 cccagttccg taaggggtac gacgagcgcg acgtcgacga tttcctcgat gaaatcgtcg
    24121 ccgagatgcg ttcgctgctt gcgcagcggg acgactacaa gcagcagttg gccgactgcc
    24181 gggccggccg cgggatgtcg atcaccgacg ccgcgaccgt tgcggcacca gcgtttgtcg
    24241 acactcgggc ggccgacgag gccgaagccc gactcgtcga ggcgaatgcg gagttggccc
    24301 aggcccagac cgagcacgct cgggtcctgg aatcgatcgg cagcgcgcgc gccgagttcg
    24361 atggcctgca gagtcggctc gatgggctgc ggggctcgtt ggccgacgcc caggcccagg
    24421 ccgatggtgg cccccggttg gtccaggacg ccgaagccgc ggttgccgac gcccagcgtc
    24481 gcgccgaaga gttcgggtcg cgggccgcag aggccgagaa gcacctctac gagatcgagg
    24541 ctcgcgcggc cgaggccgac gccaaggcgc aggaggctgc ggccagggtc agcgaggcgg
    24601 agcagcgcgc cgctgaggca caggcgagcg ccgccgacgc gcaggcacgc gccgagcagg
    24661 ctgaggcggc ggcccaggct gcggccgagc gtgagcgtca gtccatcgag gcgacccggg
    24721 tcgaggcgcc ggcggcaccc gccgcggctc tcgtcggtgc cggtgcgacg ggcgcgggaa
    24781 gcgccgcggg cctcatcgag ttggcccagc ggttgcacga cgagcacgtt gcccagggcg
    24841 aggccaagcg ccacgagctg gtctcggccg ccgagaaccg ccacaacgag ctgctcgcga
    24901 ccggccaggc ccggttcgac gagctcgtcg gcagcggcca gcgtcagcac gacgacctgg
    24961 tggcgacggc tcagagccgg cacgacgagt tggtctcgac cgctcagaac cgccacgacg
    25021 agctcgtcgg cacggcccag cgtcagcacg atgagatgct tgccgaggcc gccaacctca
    25081 aggagcagat gctcaccgag gcgcgcgagc gctcgacggg catggtcgcc gaggcgcagc
    25141 agaagaaggc cgccgttctc gaggagctcg agggccagcg cacgtcgatg gagcgcaaga
    25201 tcgacgagct gcgtggtttc gagcgcacct accgctcgca gctgcggtcc tacttcgagg
    25261 acaagctctc ggagctgacc aacaccggag ccgacgcctc cgcctgatcg cgtcacccgc
    25321 gtggccgcgg ccggacctag ttccggtatg ccgcgcagca ccacctcgac gagggcccgg
    25381 acctgttccg ggccctcgtt cggtgcccgg tctgatgatc taggtcacct tcacgtacct
    25441 agacgggcgc ggattggcgc caaacccccc tgaaaccggg caggtggcct attctcggcg
    25501 cgatcgtcca tacacccatc gggcgagcgg acaccggaag gggccggacg accatggcgg
    25561 cgacgaaggg caagaacgcc gcggctgctg ccggtcgagc gacggcaagc gtcgctcgag
    25621 cggttcgggc ggtgctgtca ggagcagtgg ctggcacccg gtcgagcgaa gccgatccat
    25681 cgggtcaccc cgacccagcc ccaggccacg cacctgtcgt caagaccagg ccggccgcga
    25741 aggcggcggc agtgaaggca acacccgcga aggcgacact ggccaagcgg gcacccgcga
    25801 aggcggcggc agtgaaggca acacccgcga aggcgacact ggccaagcgn nnnnnnnnnn
    25861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcacccgcga aggcggcggc agtgaaggca
    25981 acacccgcga aggcgacacc tgtgaaggcg acaaccagaa cggcggcgac caaggctgcc
    26041 ccggcgaagg tggcaaccgt gacgtcagac cccgcgaaga ctgtgtccgc caaggttgca
    26101 cctgccaagg ccgcacccac gaaggctgca ccggcgaagg ctgcaccggc gaaggctgca
    26161 ccggcgaagg ccgcacctgc caaggctgca ccggcgaagg ccgcgagcgc caaggccgtg
    26221 tcggcggttc ggtccgcctc cacggtcatc agctctgtgt cagcggacat gcctcccgta
    26281 cgcgaggacg aggagccgtg gaccgaagcc gaattgcggg aggtgcgcca ggagatcgag
    26341 cgggacatcg ctcgcgggct agccgacatc gccaccgccg aggaagacct ccagggcctc
    26401 atctgggatg cgggcgacgg gtctggtgat gaccaggccg acgcgggagc caagacctac
    26461 gagcgcgagc aggagatctc gctggccaat atctcccggg acaagctggc ccagaaccgg
    26521 cacgcgttgg agcggttgga caacggcagc tacgggatct gcgaggcgtg cggtctgccg
    26581 atcggcaagt ttcgactcca ggccgctccc cgtgcgaccc tatgcaggtc atgcaaggag
    26641 aaagcagagc gcgcctgagc gcaccgtccc cccgaccgac ccgagtttcc gggtcacgtc
    26701 ggctcatctg gctattcgtc gggatcgcgg tcgtggtcta cgtcctcgac caagcgagca
    26761 aggcgtgggc cctcgccgcc ctcaccaacg cccctcccca ggacctcctc ggcgccttcc
    26821 tgcgcctcta cctcgtccgt aaccccgggg ctgccttctc ggtcgggacc ggtagcacgt
    26881 ggctgctcac catcctggcc gtcgtcgtcc tcgtcgtcct catccgggcc agtcgacggc
    26941 tgggcagcat ggcctgggcc tgggccttcg gcttgatcct cggcggcgcg ctgggcaacc
    27001 tgaccgatcg gctgatccgc gacccgggct tcggccgcgg ccacgtggtg gacttcatcg
    27061 actacaacgg ctggttcgtc ggcaacgtcg ccgacatcgc catcgtcggg gccgccctgc
    27121 tgatcggcct gctcgccctg cgaggcatcg gcctggatgg acgtcgggac ggcgcgggag
    27181 gatctggtga ggcggacgga tctggtgagg cggacggcat acctgagccg cgggggacgg
    27241 aacgcgatgg gtgacgcgcg caccctgttc gttccggacg gacttgacgg tgagcgcgtc
    27301 gacgcggcca ttgcccggtt gtttgggttc tcgcgcacgc gtgcggccga actcgccgcc
    27361 gacggcatgg tcaccctcaa cgagagggtg agccccaaat cggcccgggt gagcgcgggc
    27421 gacgtcctcg aggtcgtcat cccggacgcc ccgagtgcag gcgtcgaact cgtcgccgag
    27481 ccggtccccg ggatgagcat cgtccacgac gacgacgacc tcgtcgtcgt cgacaaaccc
    27541 gtcggcgtgg cggcgcaccc gagcgtgggc tggaccggcc ccaacgtcgt ctcagggctc
    27601 gctgcggctg gctaccggat cgccacgtcg ggggccagcg agcggcaggg catcgtcagc
    27661 cgactcgatg tgggcacctc ggggctcatg gtggtcgcca agagcgaata cgcctacagc
    27721 aggctcaagc atgccttccg ttcccggacg gtcgacaaga cctaccacgc gctcgtccag
    27781 gggctccctg acccgatgac gggcaccgtg gacgcgccga tcggacgtca cccggcgcac
    27841 gagttcaagt tcgccgtcat ggcctctggg aagccggccg tcacccatta cgaggtgatc
    27901 gaggctttcc atggggcctc gctgctggag atcaagctcg agacggggcg aacccatcag
    27961 atccgggtgc acctctcggc gctgcaccac ccgtgctgcg gcgacccgct ctatggcgcc
    28021 gaccccaagg tgtcggcgcg cctcggcctg gaccggcagt ggctgcacgc cgtcgggctg
    28081 ggcttcgagc accccgggac gggtgagtgg gtcaccttcg ccagccccta tccggccgac
    28141 cttgagcacg ctctcgacgt gttggccgac tcgcccgcgc gctgagacct gccgggtcga
    28201 ggtattccgc catcgtctgc tgatagcgga atacctcgac cccgcaaggg gtcgggggtg
    28261 gacgggggcg acacgggcgg cgacacgcgc ggggtcgtcg gcggggcggc atacagttgc
    28321 tgcgatgtcc accccacccg cctccgagcg cagcttcgcg cacctgcacg tccacaccga
    28381 gtactccatg ctcgacggcg ccgcgcggat cgacgagttg ctcgacgcgg ccgtgcggat
    28441 gggtatgccg gccatcgcca ccaccgacca cggctacgtt ttcggcgcct atgagttctg
    28501 gtccaaggcc cgcaaacggg ggatcaagcc gatcatcggg gtcgaggcct atctcacgcc
    28561 gggcacccac cgcaccgaca agactcgggt ccggttcggc gacgacccgg ccaacaagga
    28621 cgacgtcggc ggcggcggcg cctacaccca catgacgctg ctggcccgca ccactggagg
    28681 catgcacaac ctcttccgga tgagctcgct ggcgtcactc gaaggccact acttcaagcc
    28741 tcggatggac cgcgacctcc tctcgaggtt ctccggcggg ctcatcgcga ccacgggctg
    28801 cgtcggcggc gaggtccaga cgaaactgcg cgtggggcag tatgccgcgg cccgcgaggc
    28861 cgccgcggag ctgcgggaca tcttcggggc tggcaacttc tatgccgagg tcatggacca
    28921 cggcctgccc atcgagcggc agacccaacg cgagttgttg cggctggcca aggacctcgg
    28981 gctgccgctg ctggcgacca acgacctgca ctacacgaag gcggaggact ccaccgcgca
    29041 cgcggcgctg ctgtgtgtgc agtcggggtc gacgctcatg gacccggggc ggttcaagtt
    29101 cgatgccgag gagttctacc tcaagtcgcc ggcgcagatg cgcgagttgt tccgtgagtt
    29161 gcccgaggcg tgcgacaaca cgttgctggt cgccgagcag tgtgacgtct ccttcaccga
    29221 ggaggagggt cgctacatgc cccgattccc ctgtccggag ggggagtcgg agaccacctg
    29281 gttcatcaag gaggtccagc gcgggctgca caagcgttac cccgccggca tcccgaccgc
    29341 cgtgcaggag cgggctgact tcgaggtcga ggtcatcctc ggcaagggtt accccggcta
    29401 cttcctcgtc gtcgccgact tcatcaactg ggccaaggac catggcatcc gggtcgggcc
    29461 gggacgtggc tctggtgccg gttcgatgtg cgcctacgcc atgggcatca ccgacctcga
    29521 cccgatcgtc cacgggctgc tgttcgagcg cttcctcaac cccgagcggc cgtcgatgcc
    29581 cgacttcgac gtcgacttcg acgagcgtcg gcggggcgag gtcatccgct acgtcaccga
    29641 cacctatggc gaggaccggg tcgcccagat cgtcacctac ggcaccatca aggccaagca
    29701 ggccgtcaag gacgccgccc gggtcatggg ccaccccttc gcgatgggcg accggctgac
    29761 caaggtcatg ccggccgacg tcatgggcaa gtcgatgccg ctgtcgggca tcttcgactc
    29821 gtcccatgcc cggtatgccg aggccgccga cttccgcaag gtccacgaag aggacccgca
    29881 ggcccgtgag gtcgtcacca ccgcgctcgg cctggagggc ctcaagcgcc agtggggagt
    29941 gcacgcagcc ggggtcatca tgtccagcga gccgctggcc gacctcatcc cgatcatgcg
    30001 ccgcgagcag gacggccaga tcatcacgca gttcgactac ccgagctgcg agaagctcgg
    30061 cctggtcaag atggacttcc tcggcctgcg caacctcacc atcctcgatg acgccatcgc
    30121 caacgtcgtc gccaaccggg gcgaggccat cgatctcgac gccctgcgcg acgccccgac
    30181 tgacgaggcc acctacgagc tgctcggccg cggtgacacc cttggggtct tccagttcga
    30241 ctccggcggc tacaagcagt tgtgccgcca gatgcgaccg accgagttct ccgacatcat
    30301 cgccctgggc gcgctctatc ggcccgggcc gatgggcacc aacacccaca cgaactacgc
    30361 gctgcgcaag aacggccagc aggcagtcga gcacatccac ccggagctgg ccggccccct
    30421 gtcgcagatc ctcggcgaga cctatgggct gctggtctac caggagcagg tgatggccat
    30481 cgcccaacag atggccggct acaccatggg cggcgccgac gcactgcgca aggcgatggg
    30541 caagaagaac ctcgctgtcc tcgagaagga gtaccccgcc ttcgaggccg ggttgatgcg
    30601 cagcgggtat ggccgggatg ccgtcaagac cctgtgggac acattggttc cattctcgaa
    30661 ctacgccttc aacaagtcgc acgccgcggc atacgggctc gtctcgtatt ggaccgccta
    30721 cctcaaggcg aacttcccgg cggaatacat ggctgccctg ctcaccagcg tccgcgacga
    30781 caaggacaag tcggcgctct atctcaacga gtgccgacac atgggcatca aggtcctgcc
    30841 gcccgacgtc aactcctcgg tcggcaactt cgcggccgtc ggctccgaca tccgcttcgg
    30901 gctcgaggcg atccgcaacg tcgggcgcaa cgtcgtcgag tcgatcatcg ccgcgcgcac
    30961 cgagaagggg gacttcacct cgttcgagga cttcctgcgc aagtgccccg ccaccgtcct
    31021 caacaagcgc accatcgagt cgctcgtcaa ggcgggagcc ttcgattccc tcgggcacca
    31081 gcgcgccggg ctggtgcgca tccacgaggc ctacgtcgat gccctcgtcg atgagaagcg
    31141 gcaggaggcg atcggtcagg actccctctt cggcggcttc ggtttcggcg gcgatgacgg
    31201 tgaggcggtc caactcgtcg tgctcccgcc gatccccgag gtcgagtggg acaaggcgac
    31261 cctgctggcc ttcgagcggg agatgctcgg cctctatgtc tccgaccacc cgctgttcgg
    31321 catcgagcac gtcctggccg cgcacgccga cacctcgatc gcctcgctca tgggtgacga
    31381 gccccgccct gacgggaccc aagtcaccgt cgccgggctg gtgaccaacc tgcagctcaa
    31441 gcgcacgaag aagggcgacc tctgggcgat cgccacggtc gaggacctcg acggtgccat
    31501 cgagtgcctg ttcttcccgt cgtcctacca gaacgtcgcc acccggctgg tgcccgacct
    31561 ggtcgtgtcg gtccgcggcc gggtcaacgc ccgcgacgac acggtgtcga tctacgccca
    31621 ggagctcaac gtccccgacc tcaccgatgg cccgcgcggc ccggtcgtcc tctcgatgag
    31681 cttgcgtcag gcgaccggcg cggggatcga acggttgcgg gacatcctcg gccagcaccc
    31741 cggcgtcacc gaggtccacg tccggctcaa ccagcccggc cgctcggtcc tcatgcgcct
    31801 ggacgacacc taccgcgtca ctccgtcacc cgctctctac ggcgacctca aggccctcct
    31861 cggtcaaggg tgcctcgcct gagcggcctg gtcgcggggc ggttcccgcg cgcacggtgc
    31921 cgggccgcgc ggcatacccg aggcaggggt atgccgtgac gccggctcag cgtcgcgacc
    31981 tcgccttccg gcggctggcc gcgctgcggc tggccccgct cgccccggtc gctgcgcgca
    32041 ccccgctcgc cgtcgttgag catcacctgg ccatgcaggc ccaggacctg tccagcggac
    32101 tctggtcgat cggcgtgcga accggcggca ctgtgtcgga tgtccttgcc tccgtcgagc
    32161 ggggcgagct cacccgcacc tggccgatgc gcggcaccct gcactgggtg gccaccgccg
    32221 acgccggctg gttgaccgcc ctgttggccg ccccgtcgat gcgggcggcc gcacgggtgc
    32281 tggagcgcga aggcgtcatt gacgaggtgg tcgagcgagc cggccggctg tggaccgagc
    32341 acctcactgg cgccggagcg atgacccggg ccgaggcgtc cgccgcgctg accgcgggtg
    32401 gcg
//
LOCUS       DAORPW010000010        31347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_208_length_31347_cov_752.831650, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000010 DAORPW010000000
VERSION     DAORPW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 31347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31347
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208_length_31347_cov_752.831650"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2575)
                     /locus_tag="PLB86_01880"
     CDS             complement(<1..2575)
                     /locus_tag="PLB86_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010552273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HRC63917.1"
                     /translation="MTTQVERPPAPEPTGLDGPLSDALIGTRRFFAKAKGATVSDDGR
                     TLHQIGGREGDSFYRDRWAHDKVVRSTHGVNCTGSCSWKVYVKDGIITWEAQQTDYPS
                     AGPDRPEYEPRGCPRGAAFSWYTYSPTRVRYPYVRGVLLEQFRAAKAVVGDPVVAWAA
                     IQQDPATRAAYTSARGKGGLVRASWDEVVEMVAAAHVYTVKRWGPDRIAGFSPIPAMS
                     PVSYASGARFLELIGAPMLSFYDWYADLPNASPQMFGDQTDVPESGDWWDAGYLIMWG
                     SNVPMTRTPDAHWMTEARYRGQKVVAVAPDYAENVKFADEWVPCRPGVDGALAMGMGH
                     VLLKEFFADRQVEFFEDYNRRYTDLPFLVSLDRHDETCYTAGKFVVAGDLDVPEKDSE
                     NALWKPVVLDARTDEVAVPHGSVGFRYGDEGLGKWNLDLGEIEPRLSMLGAHDDTALV
                     RFPRFDSEGGLVAFETRGVPVRTIDGRIVTTVLDLLLAHYGVARDGLPGQWPTGYADA
                     SVPATPAWAAEITGVPAEQITRLAREWAQNAIDTKGRGMILMGAGTNHWFHSDQIYRA
                     MLLLTTITGCQGRNGGGWAHYVGQEKVRPIMGFQHLAFALDWHRPPRHMNQTAYWYVN
                     TSQYRYDTFTADDLDAGTGVFAGKGVMDLLAQSVRLGWTPSYPTFNRSSLALADEAKA
                     AGQEPAAYVVEQLRTGALTFAVEDPEAPENHPRVLSLWRSNLLGSSAKGNEYFLKHLL
                     GTDHAVTAAEAAPDQRPDGIEWPEHAAEAKLDLLMTIDFRMTSSTMFSDIVLPAATWY
                     EKHDLSTTDMHPFVHSFSPAIDPPWEARTDWGIWKAIAKKFSSLAVDHLGTRTDVVAK
                     PL"
     gene            complement(2572..3324)
                     /locus_tag="PLB86_01885"
     CDS             complement(2572..3324)
                     /locus_tag="PLB86_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC63918.1"
                     /translation="MSPEQIPVLSSDVRRRILEHLNVVAGQAPPGAPTGRSAADLAAY
                     LGLHVTTVRFHLDQLVTGGYLVTAFERTPRVGRPRKLYAAPHLDPDAETETQRHYQVL
                     AGLLAQSWPVDGGDQVTPEEAGRRWASGHADPSRASTEAAVTPGRWLAKVGDMVDQLE
                     HWGYTPELSTSAGGRAVTITLSNCPFLALARVRPDVVCGVHHGLITGALDHVGEHDVA
                     IEVRPFVGPTRCLVKVTTETPFTTAAPEGESR"
     gene            3641..4915
                     /locus_tag="PLB86_01890"
     CDS             3641..4915
                     /locus_tag="PLB86_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63919.1"
                     /translation="MSTAAFTAMFAVWLMFGILGPSIQKEFGLTDVELSWITAVAVLN
                     GSMWRLPAGMLTDRIGGRKVTVFMLAATAIPAYFVSTASSYGMLLVLAFLVGFAGNLF
                     SVGIAWNAAWYGRERQGFALGVFGAGNVGASVTKFIGPPIILGTAGSSFAFGLQGGWR
                     LVPVIYSVLLVVLAVLTYIVVPRRDRMPGATKPVREMLTPLKQVRVWRFSLYYVAVFG
                     AYVALSAWLPKYYIDNFGVEIWPAALLTATFIFPASLLRPLGGWVSDRVGARRVMYWT
                     FGVMLVTTGILMMPNGHIVVSHPDGSQTTHLGYQIGLPTFAILVFFLGVAMGIGKAAV
                     YKHIPEYFPDNVGSVGGLVGMLGGLGGFFLPPMFAYTKAWTGFPSSTFACLFIVTAIC
                     AVWMHWTVVHMLHSGSPELADHFDTEHVSTRG"
     gene            4995..6392
                     /locus_tag="PLB86_01895"
     CDS             4995..6392
                     /locus_tag="PLB86_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63920.1"
                     /translation="MSSTPTTPRSGEWLDAWDPENEATWDKALAWKTLWITTFTLTMA
                     FVAWFLPSAIVPKLNALGYTFSKEQLYWLAAIPGLSGGLMRIVWMILPPIMGTRRMVA
                     LTTALLFFPVLGWGVRVQSPTAPYWELLVLAFLAGIGGGAFSGFMPSTSYFFPRRLQG
                     TALGLQAGIGNFGVSLVQLMTPWLIGFGMVGFLGASQQMKVPGKPDVTVWYQNAAFFY
                     IPFIIVGTIIAWTMLKSVPIKANIRQQFDIFRTPDTWWMTLLYIMTFGTFSGLSAQFG
                     LLMTNLYGAGNAAIVSGSGSTATLLVAGYAVPDAVKYVFLGPLVGAGARILFSPLTDR
                     MGGARWTLVSGIGLVGSIIYTIGGLVPDTSSAEALQAGFGRFLWGMLAIFLFAGIGNA
                     STFKQMPMIFERRQAGGVIGWTGAIAAFGPFLFGVGLTLMSPTAFYWIGAAFAVLCIG
                     ITWVRYARPGAPKVS"
     gene            6715..7368
                     /locus_tag="PLB86_01900"
     CDS             6715..7368
                     /locus_tag="PLB86_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63921.1"
                     /translation="MSTLDTTSATTHRRSALLAPVGVLAVVAGVIGWMLPDRTVGTGA
                     MDGMSMTHYMGLLAVNQPWNLILFMAIPVILAETLAITELVLLFRSDPPTWVRSLSRW
                     AGLIAGPVMVLVLVHLLKNAVVPLTSGGGWRGAADVIAVLTYLLAGLPLIGITLVEVG
                     AIGTDARDARKWHAVFVGVFLVLAHVAMIFGMLDPTVLGWGATHDMPGGGTMPGMNH"
     gene            7458..7679
                     /locus_tag="PLB86_01905"
     CDS             7458..7679
                     /locus_tag="PLB86_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRC63922.1"
                     /translation="MATQSFQVTGMTCGHCAGHVTAELKSVAGVSDVTVDLVAGGTST
                     VHVDADHELTDAEVTAALDEAGNYALAGR"
     gene            7758..>9408
                     /locus_tag="PLB86_01910"
     CDS             7758..>9408
                     /locus_tag="PLB86_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRC63923.1"
                     /translation="MTAGPDTQAPPSELQQTQLVVGGMTCASCSARVEKKLGKLTGVT
                     AAVNYATGKAFVVHPGGLAVADLVAAVESAGYTATPAPPSIPGSQPGPGEGGEPDADD
                     EHAAYLRQRLLVALGLSVPAIAISMVPALAFPGWPWVVLALTLPVVTWCAWPFHRAAW
                     VNLRHGATTMDTLVSGGVLAAFGWSLWMLLTMGAHGHFYFETAAAITTFLLAGRYAEA
                     KAKRRSGAALRALLHLGAKDVAVLRGDVEVRIPLGELLVGDEFVVRPGEKVATDGVIV
                     DGASAVDASLLTGESVPVEVGVGDPVTGATVNTSGRLRVRATRVGGDTQLAQMARLVE
                     SAQSGKAAVQRLADRVSAVFVPVVLVLAALTLIGWLVTGHSAESAFTAAVAVLVIACP
                     CALGLATPTALLVGTGRGAQLGILIKGPEVLESTRRVDTVVLDKTGTVTTGRMAVTGV
                     YAAPGQSPERVLALAGAVESGSEHPIARAVTSAASAPGLRLSEFRNHGGLGVEGVVDG
                     MPVRVGRLSFVTSAPATGQGVDVSLSEAAEEAMAQGQTPVWV"
     assembly_gap    9409..9508
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9509..10153
                     /locus_tag="PLB86_01915"
     CDS             <9509..10153
                     /locus_tag="PLB86_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRC63924.1"
                     /translation="LSEAAEEAMAQGQTPVWVGWDGTAQGVVVVADTVKPSSARAVEL
                     FRGLGLQPVLLTGDNARAAAAVARHTGIAPEDVRAEVLPAEKVAVVAALQAQGKVVAM
                     VGDGVNDAAALAQADLGIAIGTGSDVAIEASDLTLVRGDLVSAVDAVRLARATLRTIK
                     GNLFWAFAYNVAAIPLAASGRLTPMIAGAAMAFSSVFVVTNSLRLRRFKPSISA"
     gene            10193..10948
                     /locus_tag="PLB86_01920"
     CDS             10193..10948
                     /locus_tag="PLB86_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC63925.1"
                     /translation="MTIPCITVGSGEHHVFVLHGWFGSADGWGLFPTYLDGERFSYHF
                     TDNRGYGARMDERGHYSLDEVADDVLALADSLDVQTFSLIGHSMGGAEVLRILAKAPG
                     RVRKLVGLTPVSASPTPFDEAGHDLFWGAAEDPGKRFGIIDFTTGNRNTATWVQQVVD
                     HSLSHSTTEGFAGALEAWAGPDFLAEIAGTQTPMLVIAGEHDPALGEATVRQTWEPHF
                     PQCEIHVMANAGHYPMFETPVQLATVIERFLAD"
     gene            10965..12368
                     /locus_tag="PLB86_01925"
     CDS             10965..12368
                     /locus_tag="PLB86_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRC63926.1"
                     /translation="MSTVDRSAARARVDAAYARLTEVERGWTRVVHDPATYTRDVPYA
                     ALAGLRDRHGVVPVAEPALPGWPEGTGYWLVLRHADVDRVLKDPATFSSSLGGTQMRD
                     PRTEDDLAYVRRMMLNMDPPEHSRLRRLLAKSFTPKAIGALEAQIQGHVTGILDQALD
                     STPTSRQGACDFAKDVSADLPLLTLADVMGVPAPDRWLMFDWANRVIGWQDPDYRVSA
                     AFDPSGATELGRAAYALRPSPGADGLMPDPRTREGMPDLYAYAHALGTLRREQPGEDV
                     MSILMSQRDDAGGSVSVAEFENLFWLFAVAGNETTRNGMPGGLLGLLGSPQAQEALRA
                     RPDGIPNAVEEMLRWWTPVMTFRRTATCEVELSGVTLQRGDKVVVSFAAANRDPEVFA
                     DPDVFDIERANARRHLAFGAGPHVCLGAFLARSMMTTMFTQLLGRTTWLEAAGEPVWL
                     QSNFQRGVKRLPITWRM"
     gene            12439..12675
                     /locus_tag="PLB86_01930"
     CDS             12439..12675
                     /locus_tag="PLB86_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRC63927.1"
                     /translation="MTTTVGAYEAKTHLPALLAQVERGETVTITRHGRPIARLVPIDV
                     HRPVEEIILELDRLRRGVTTGGMSIREMIEEGRR"
     gene            12675..13079
                     /locus_tag="PLB86_01935"
     CDS             12675..13079
                     /locus_tag="PLB86_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRC63928.1"
                     /translation="MAFVLDSSVALSWCFEDEVTEATLRIRERLSTERAVVPSLWLLE
                     VANVLLLGARRGRLTAEAAAEFADLLVGLPIDVVEPERDIADLMQLAAEHRLTAYDAA
                     YLQVAAARRLPLATLDATLAEAARAAGVDLLG"
     gene            13165..14556
                     /locus_tag="PLB86_01940"
     CDS             13165..14556
                     /locus_tag="PLB86_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRC63929.1"
                     /translation="MTDVTTEPAAPTTFRVPTDQATVRANDRQHVFHSWSAQGAITPL
                     PIARAQGSRFWDYDGKDYLDFSSGLVHANIGYGHPKLVAAVQEQAGRMATIAPGYAVD
                     VRSEAARLIASKAPDGLEMVFFTNGGAEATENAIRMARLHTGRHKVFAAYRSYHGATA
                     GALTMTGDPRRIPSEPGLPGIIHFWGPYPYRSAFHSTSPEQESERALGHLRDMIMVEG
                     PAAVAAIILETVVGTNGVLVPPPGYLAGVRAICDEFGIVMIADEVMAGFARCGEWFAI
                     QHWGVTPDLITFAKGVNSGYVPIGGVIISEAIAATFRDRVYPGGLTYSGHPLACASAV
                     ASIQIFEEEGIVEHARMLGTDVIGPALTELAQRHPSIGDVRGLGAMWAVELVRNRETR
                     EPLVPYNATGAAAAPMTEFAAACKARGLWPFVHFNRTHVVPPCTTSVEEVREGLAILD
                     EALTVADGYVTTD"
     gene            14613..17024
                     /locus_tag="PLB86_01945"
     CDS             14613..17024
                     /locus_tag="PLB86_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRC63930.1"
                     /translation="MRQDGAVASSSGRRPVSVEVVEPHMADRVRRASDVSWFLVYAVL
                     AALSITLGNLAVGTAGALEQDLALATGGLPRLLLQLFSWVAGVGVLFLPIAVGVDMLL
                     RSRGWQLVQALLAGGLAAGIAMLIKAAILDGQFTQVLAALTRPLNSNGRTNPLDVLVV
                     TLVALVVVANISGRRWLATLAPLVIGSLLVTGFLAGSNTALALLCSFLLGAMVGHALR
                     FAFGTASTRAPGTAIARELVNAGTPLQTLELVDEYEEGARLYRGLTPAGEIDVHVFDR
                     DTFGLASGRRLLNRLRLRGATARAPSFTLRSALEHRSLQALALRWAGVWAPFPIAVCD
                     VDGATAVLALTRVEGVSLRVLGDNLTDAQARAVVRLLVALQEHRIAFRGLTRDNIVIL
                     PDGAAGLLSVGEGDVGGDDIARRADAAQVMVMLALAIGPERAVALAVDEAGSRTVSRT
                     LPLLQALALGRELRGELKARKGLLDELREQVLTLEDSHNQPATIELRRVTVKGLLTLV
                     GGAVAAYIVLPQLAQVDFAEVLSRAQWPWALGSVLAAVLTFIGASLVLTGSVPTKLRF
                     RHTFMTQLSVAFSGLVAPAAIGNLALNTRYLQRAGVPAGAAGASVGLAQVFQFTSYVT
                     LLAVTGVVAGISPQASFQPPAKVVAAIPVVLVIVIGLFAVPRVRRFFRERLLPQLRQV
                     LPQVLAVLQRPRKLAELLGGALLLDISFVAALYCATYAFGAAPPLAGVAVVYFAGAII
                     GSAVPTPGGLGGIEAAMSYGLTTLGVDSGTAVSSVLLYRLATYWLPIPFGWASINHLT
                     KIDAI"
     gene            complement(17038..>17495)
                     /locus_tag="PLB86_01950"
     CDS             complement(17038..>17495)
                     /locus_tag="PLB86_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRC63931.1"
                     /translation="TATGPGVPPAIALEWVAEVWIDPDWYAVQEADEPMPSAGLPVVV
                     PLLVRSALIGRVSASKHIVPDIDCTSDSGVSRRHAQLTTDGTRWFVEDLGSSNGTFVG
                     DAGDALPENPIDVGPKQELGDDDRIYVGAWTRIVVREATDEEKAAAAAG"
     assembly_gap    17496..17595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17596..17866)
                     /locus_tag="PLB86_01955"
     CDS             complement(<17596..17866)
                     /locus_tag="PLB86_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRC63932.1"
                     /translation="MSYVCPNGHDSTAADFCDTCGAKIDAAAQGLVPEAAQEATPEAS
                     APAAPTSLACPNCATENVPESLFCEACGYDFTTGAMPRGSEPEGES"
     gene            complement(17863..19149)
                     /locus_tag="PLB86_01960"
     CDS             complement(17863..19149)
                     /locus_tag="PLB86_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC63933.1"
                     /translation="MAQFTTEVFQNEFLPDGGTDVHAIVRVTCSGAGKAGIGGGTGGG
                     EAAEILIVDTSGSMGANGIMQGRYAATVALNEITDGTWFAIIAGNHVGTLVYPAFGAT
                     QMVRMDPRTRQEAIASIQQFRADGGTAMGTWLTKAKEVFLTVPHVVQRHAILLTDGTN
                     EHETPDQLTWAIQGATGFFQCDVRGLGDRWQIDEARRIASALLGTVDLIPSPERMADE
                     FEKLIRASMARGVASVDLRVWAPQGAQVLFVKQVAPNLEDITAKRSQVNPLTAAFPTG
                     AWADETRDYHVAVRLPAKSLGQEQLASRVQLVVRDEVQAQALVKATWSNDEALTTRID
                     DQVARYTGQAELAAAIQDGLAAKAAGDDAGATRKLGRAVQIATATGDEEMTSRLKKVV
                     EIDNAEAGTVRLRKSVEKLDEMALDTASTKTRRIKK"
     gene            complement(19130..20542)
                     /locus_tag="PLB86_01965"
     CDS             complement(19130..20542)
                     /locus_tag="PLB86_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HRC63934.1"
                     /translation="MSAPVAPAAPPAAAGTCPACGAERAADAAFCEECGHDFGADTST
                     PAAAPVGTTGESQTRHAPVRAQTAAAGEESPLDLGWTGPVSRGAAAEVDPPAAPRCAQ
                     CGTGSFLDGYCDTCGAKAPDPRDHFTEEPVAWIGGVCDIGRRHSRNEDAMALMGSARP
                     LTQAVLVVCDGVSNTTDSHIASLAAARAGREVLDDPVPRGVGLPEAVVGTISKRLGEA
                     VVAARAAVLEATHNPADETPPSCTFVAALIDEGLAVVGNVGDSRAYWLPDAPASQPRQ
                     LSQDDSFAAEQMREGMPRKDAETGPNAHAITRWLGIDSPDDLTPHTASLQLVEDGWLM
                     LCSDGLWNYCSDAAALRDLVATTVAGLGSAGLHPPTLAQALVDFANAQGGIDNITVAL
                     ARVGPTSGPPESAPPASTQEPPPDAEPAPAAYPASEPVTASATPTAEPAPPSLPAPAP
                     APASSTGADREDSPDGTVHH"
     gene            complement(20539..22980)
                     /locus_tag="PLB86_01970"
     CDS             complement(20539..22980)
                     /locus_tag="PLB86_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC63935.1"
                     /translation="MTTPAKCQEPGCTGSILDGYCDVCGTPGATSAGAASTGVASPEA
                     AAAGAAAAALAAEEEPITPAARPQPQAASAVPNGTPCAQPGCQGHILDGYCDVCGTAA
                     DSDPAESAADPSPMGSTRSVASNRLSSTAIGSARARQTGTKATRRVGSSSQRMRSARL
                     GAGLTVIPPMPAIDAATAVMQNPVVPEDKRFCSNCGTKVGRSQGDRPGRTTGFCPKCR
                     QAFSFDPKLQPGDLVAGQYDVAGCLAHGGLGWIYLARDRNVSNRWVVLKGLLNTGDPD
                     ALAAAIAEQRFLAQVEHPRIVEIYNFVMHEGAGYIVMEYVGGTSLKALLKQRMRKTGR
                     YDPLPVDQALAYILEILPAFQYLHDLGLAYCDFKPDNVIQVGDDLKLIDLGGVRRMDD
                     DDSAIFGTLGYQAPEVAEQGVSVASDIYTIGRSLVVLTTEFRGYQTTYVDSLPPVEDT
                     PVFARYDSFYRLLLKACAKSPDDRFASADELRVQMLGVLREAVAQDAKVGRATTSATS
                     LLFEEPSISTDRLEWWSLPRLRPDPHDAQAALVRDVRSLEPHERFEPLRAADPATPDV
                     QLELARTALDRGDAGVVDAATNTLLHEDPWEWRAVWIQGLAALARRDTAAAQAAFNAV
                     YGQVPGELAPKLALAFACEVSGEAAIAESLYAVCARTDSNYTPVGAFGMARIRRAEGG
                     IPAAVEALDLIPVTSRAYALARHQRGQLLADSGQGLPALAEALRSVDGVPLDASDKAG
                     LTVQVLENALTWVTSPQGGPNPGITLAGHPASVSGVRQGLEAAYRQLAEVTPDPAART
                     ALVDQANAVRPWSVL"
     gene            complement(22977..24203)
                     /locus_tag="PLB86_01975"
     CDS             complement(22977..24203)
                     /locus_tag="PLB86_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63936.1"
                     /translation="MSLSLDPIPQPAAGGALPGAAAPEAPGRLGTPLGVHEAYAYLRD
                     LGVWRDKRKAELDSLDAAALASTDRDSFSRDMLVSMALWKAISDRFDLLTVTFDSGRV
                     GPTEAARLSTLIWGRLDVAPDSAAATISPTTAGALGLSLPEACRLCDAMTSSLRARLS
                     LEPSGAEVGTRLKDVRATIERIRDQVAIAPAGVDREAARALLTRLEQRLVDVTDRAKR
                     GAEVGGLLAPLELEAAGAERDLIVGAGRRVETARDAERAAQVRDELLARSAAVQQLVD
                     RCVAAVTPAPRLAVPDVRALGPVPSGGAAVDTYLKRLDAVGRALTVVHTAYGDPLAER
                     DEIAGYAGALAAQARATGVSDPDLDHLRERLQTVVDTTPTDLRRARALRDAYQAYAMS
                     LRTTDTSSGRKGASPR"
     gene            complement(24217..25242)
                     /locus_tag="PLB86_01980"
     CDS             complement(24217..25242)
                     /locus_tag="PLB86_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRC63937.1"
                     /translation="MSRMPRTRLPLWAAGTTLLLSLAACGAGGYAQTPLSPTPTAAAT
                     TSAAPTTAAPTPAASKCNPVPAGFNPLQTYDPLPSLPASGALTGRLAEIRARGYLIAG
                     VSADTLLMGARNPLTGQIEGFDIDLVHEIAKAILGDAAKVQLVVITAGQRQSFLVNRT
                     VDIVVRNMTMNCARWLDWPIAFSAEYYHSGQKVLVRKGSTATSLDDLAAAKAKVCAPT
                     GTTSLAKLQEFVAKGVVPVTADTHTGCLLHFQQAKVDAITGDDTVLAGLATQDPYAVV
                     PAQTAITAEPYGVGFNSEDKVFVQYVNRLLETMKADGRWKASYDRWLAPSLGDAPTPP
                     TAVYGRQ"
     gene            complement(25239..26681)
                     /locus_tag="PLB86_01985"
     CDS             complement(25239..26681)
                     /locus_tag="PLB86_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63938.1"
                     /translation="MSQNPQARSRGPAAAAAAVAPPPPSASGPGAETVAPAVAMPAPA
                     AGGVRPAYANSAGAPAKARVGTWVRGKASGTPGRMRLFGALAAIAAIVFGLAGAWNLW
                     SSAQALGRAGATTDQVVRVQSIYADVLRADADASNAFLVGGLEDPAQRADYEAAIKRA
                     ATTITAAASAQPADGVALAALNSALQDYAALVERARAENRQGLPIGVTYMTSASSGLR
                     TTAVPILEALLQANTERSRGELGAASSSWPTLVAGVLAVLVLGLVSIWLARRTHRYLN
                     SGLAGATVAVLVALISGLVVFNGIGSDAKRVADNQLAGTLALTSARTAAFDARANESL
                     GLIRRGQAGPFETLWKARDAEVTARLKALVGRVSWYGGSGTNASALSTAWSAYEKAHA
                     EVRTLDDGGSWDAAVAKATTATESPRTTFDAFQAASATAVDGFQSALTGDIEGPTTKA
                     RIAALVLLLAGLAAAVLSTRGIAQRVEEYR"
     gene            complement(26678..28243)
                     /locus_tag="PLB86_01990"
     CDS             complement(26678..28243)
                     /locus_tag="PLB86_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC63939.1"
                     /translation="MSQLREAVDALVAVATEAGLPPDRARDEAMVFAAAIAEAAPRAA
                     QDWCAAIEGAPTELAAVNAAFFDAASRGRKSRGAPTALLSELAATAPGRATAYAKALT
                     EVASAACSLGEPTLRVTGNAAVAAAAQLEAARLADPGATSRGLPPGVPGMPPSPTSEW
                     DRIWPTPTLTPFPWSTPNGIPGTPVTPGTPSQPSSGGDQAQVAGDPVDAAPPAEPEPP
                     KPTKTLEELLADLDAMIGLERVKREVHQQVAMLKVDKLRREAGLKNADITRHLVFVGN
                     PGTGKTTVARMVGGIYQSLGLLSKGQLVEVDRSELVAGYLGQTAIKTAETCAKAYGGV
                     LFIDEAYALSGDQYGQEAVNTLVKEMEDHRDDLVVIVAGYPDPMVAFIAQNPGLSSRF
                     KTVIEFEDYTDDEIVAILHKLATAADYTVTPEAEGHFRSVLAATARNEAFGNGRFARN
                     ALEEAIGRQAWRLHQDADLTSDQLRELRVEDFRPHVDPEPATTDIVAFGDDPADESSE
                     PPEDSTDEGATPS"
     gene            complement(28244..28909)
                     /locus_tag="PLB86_01995"
     CDS             complement(28244..28909)
                     /locus_tag="PLB86_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63940.1"
                     /translation="MGIGEWLFGGREEQAPVTPIPRVPTADDLLAAVDRVEAMAVAGK
                     VRPEVLARLRRVTKTVRETIPRMDRLGGGSTQAYHVMATATDYLPEAIGGYLRLPREW
                     ADSRPVDRGKTPLMILVDQLDLLGRTMDRVFDAINRADAEALVIHGRFLQEKFGHAST
                     GGELALGSATPLPTMPTTPTMPPAPTPTATQTPTAPTPPATQTPATGTPQVRLQPPIG
                     RQG"
     gene            complement(29009..30268)
                     /locus_tag="PLB86_02000"
     CDS             complement(29009..30268)
                     /locus_tag="PLB86_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HRC63941.1"
                     /translation="MSQPTYGAQDASASNAAAVQPLAPPAANDSLRLEAPPAAAAPIS
                     TTAAPRMAPAVPEAALPGLDAKVENYLQALMTAAPKSPQFETKANDVRVMGDEDIRRA
                     AESSNRLLRSPVKALQEGGLSEGGKVGKTLLELRRTVEDLDPSQATGVKKVLGMIPFG
                     DKVTDYFRKYQSAQSHLDGILQALRQGQDELQRDNVALNLEKQNLWDSMGRLNQYVYI
                     AERLDAKMAAQIATLDVTDPEKATALKQDVLFYVRQKHQDLLTQLAVSIQNYLAIDIT
                     IKNNIELVKGVDRASTTTVSALRTAVIVAQALGNQKLVLDQITALNTTTSGLIQSTSE
                     MLRDNSVRIQEQAASATIGIEQLQAAFSNIYQTMDAIDTFKVQALDNMAATIGTLETE
                     VAKSRTYLERANRVNPNVQMGSLDLGR"
     gene            complement(30466..31323)
                     /locus_tag="PLB86_02005"
     CDS             complement(30466..31323)
                     /locus_tag="PLB86_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRC63942.1"
                     /translation="MLWIAVPLGLLTGAVMGALGGGGAILTIPVLIYVVGMPPHDATT
                     ASLVIIGVSAAATAISHIRGGTARVPEAVILAAVGTIGTYAGSLASAAVAAPVLVVLL
                     ASLLAVVGALMLRQSSAVIEDLGSLIDGAAEGVADGADDGAVGGRHRPPPADDRAGGH
                     RVRRFGPVAAVGIGVGLLTGFFGVGGGFAIVPALTLVLGYPMRAAIGTSLLVIALNSA
                     TALGARVIQGVSLDWPLVGTFTLGAIVGGLIGGRLARRASPETLRRGFAMLLFLVAAY
                     MLGSAVAHR"
BASE COUNT         4296 a        10571 c        11234 g         5046 t
ORIGIN      
        1 acaggggctt ggcgacgacg tcggtgcggg tgccgaggtg gtcgaccgcg agcgatgaga
       61 acttcttggc gatcgccttc cagatgcccc agtcggtgcg ggcctcccac ggcgggtcga
      121 tcgccgggct gaacgagtgg acgaacggat gcatgtcggt ggtcgacagg tcgtgcttct
      181 cgtaccacgt cgcggcgggc aggacgatgt cggagaacat cgtcgagctc gtcatccgga
      241 agtcgatcgt catcagcaga tcgagcttgg cctcggcggc gtgctccggc cactcgatgc
      301 cgtcggggcg ctgatcgggg gcggcttcgg cggccgtgac cgcgtggtcg gtgccgagca
      361 ggtgcttgag gaagtactcg ttgcccttcg cgctggagcc cagcaggttg gaccgccaca
      421 ggctgaggac acgtgggtgg ttctccggcg cttccgggtc ctcgactgcg aacgtcagtg
      481 cgcccgtgcg cagttgctcg acgacgtatg ccgcgggctc ctgtcccgct gccttcgcct
      541 cgtcggcgag ggccaggctg ctgcggttga aggtcggata actcggcgtc cagcccaggc
      601 gcaccgactg ggccagcagg tccatcacgc ccttgcccgc gaagactccg gtgccggcgt
      661 ccaggtcgtc ggccgtgaag gtgtcgtagc ggtactggct ggtgttgacg taccaatagg
      721 ccgtctggtt catgtgtcgc ggcggccggt gccagtcgag ggcgaaggcc aggtgctgga
      781 agcccatgat cgggcggacc ttctcctggc cgacgtagtg ggcccacccg ccaccgttgc
      841 gcccttggca cccggtgatc gtcgtgagca acaacatggc ccgatagatc tgatcggagt
      901 ggaaccagtg gttggtgccc gcgcccatga ggatcatgcc gcgacccttg gtgtcgatcg
      961 cgttctgcgc ccattcgcgt gccagtcggg tgatctgctc ggcaggcacg ccggtgatct
     1021 ccgcggccca ggcgggggtc gcgggaaccg acgcatcggc ataccctgtc ggccactggc
     1081 ccggcagtcc gtcgcgcgcc accccgtagt gcgcgagcag caggtcaagg acggtcgtga
     1141 cgatgcggcc gtcgatcgtg cgcaccggca cgcctcgggt ctcgaaggcc accaggccgc
     1201 cctcggagtc gaaccgcggg aaccgcacca gcgccgtgtc atcgtgagcg ccgagcatgg
     1261 agagtcgggg ctcgatctcg ccgaggtcga ggttccactt gcccagaccc tcgtcgccgt
     1321 aacggaaccc caccgacccg tggggcaccg cgacctcgtc ggtccgggcg tcgaggacga
     1381 cgggcttcca cagcgcgttc tcggagtcct tctcgggcac atccaggtcg ccagcgacga
     1441 cgaatttgcc tgcggtgtaa cacgtctcgt cgtgacggtc gagtgacacg aggaacggca
     1501 ggtcggtgta acgacggttg tagtcctcga agaactcgac ctgtcggtcg gcgaagaact
     1561 ccttgagcag gacgtgaccc atgcccatcg ccagcgcgcc gtcgacgccc ggtcggcagg
     1621 gaacccactc gtcggcgaac ttgacgttct cggcgtagtc cggtgccacg gccacgacct
     1681 tctggccccg gtagcgggcc tcggtcatcc agtgcgcgtc gggggtgcgg gtcatgggga
     1741 cgttcgagcc ccacatgatg aggtagcccg cgtcccacca gtcgcccgat tcggggacgt
     1801 cggtctggtc gccgaacatc tgcggggagg cgttgggcag atcggcatac cagtcgtaga
     1861 acgacagcat cggtgcgccg atgagctcga ggaaccgcgc gcccgacgcg taggacacgg
     1921 gggacatcgc ggggatcggc gagaagcccg cgatccggtc cggtccccag cgcttgacgg
     1981 tgtagacgtg cgctgcggcg accatctcga cgacttcgtc ccagctcgcc cgcaccagcc
     2041 cgcccttgcc gcgcgcgctg gtgtatgccg cccgcgtcgc cggatcctgc tggatcgccg
     2101 cccaggcgac gaccggatcg ccgacgacgg ccttggcggc gcggaactgc tcgagcagga
     2161 cgccgcggac gtaggggtag cgaacccggg tgggggagta ggtgtaccag gagaaggcgg
     2221 cgccacgagg gcagccgcgc ggctcgtatt cggggcggtc cgggccggcc gacgggtagt
     2281 cggtctgctg ggcctcccag gtgatgatcc cgtccttgac gtagaccttc cacgagcagg
     2341 acccggtgca gttgacgccg tgggtcgagc ggacgacctt gtcgtgggcc cagcggtccc
     2401 ggtagaagct gtcgccctca cgccccccga tctggtgcag ggtgcggccg tcgtcggaca
     2461 ccgtggcgcc tttggccttg gcgaaaaagc ggcgcgtgcc gatcagggcg tccgagaggg
     2521 ggccgtccag accggtcggc tcgggtgcgg ggggacgctc cacctgggtg gtcatcgact
     2581 ctctccttcg ggagcagcgg tggtgaatgg ggtctcggtg gtgaccttga ccaagcagcg
     2641 ggtcggtccg acgaagggcc ggacctcgat ggcgacgtcg tgctcgccga cgtggtcgag
     2701 cgcgccggtg atgaggccgt gatggacgcc gcagacgacg tcgggacgca cccgcgccag
     2761 cgcgaggaag gggcagttgc tcaaggtgat ggtgacggcg cggccaccgg ccgaggtcga
     2821 caactccggc gtgtagcccc agtgctccaa ctggtcgacc atgtcgccga ccttggcaag
     2881 ccaccgaccg ggggtcacgg cggcctcagt ggaggcgcgg ctcggatcgg cgtgcccgga
     2941 ggcccaccgg cggccggcct cctcgggggt cacctgatcg ccaccatcga cgggccaact
     3001 ctgggcgagc agcccggcca gcacctggta gtggcgctgg gtctcagtct cggcgtccgg
     3061 gtccaggtgc ggggcggcat acaacttgcg ggggcgtccc acccgcggag tgcgctcgaa
     3121 cgcggtgacg aggtagccgc cggtcacgag ttggtcgaga tggaaccgga ctgtggtgac
     3181 gtgcagcccg aggtatgccg cgaggtcggc ggcgctgcgc ccggtcggcg ctcctggggg
     3241 ggcttgaccg gccacgacgt tgaggtgttc gaggattcgt cggcgcacgt cggaggacag
     3301 gaccgggatc tgctcggggc tcacgaggcc tggtctcctt aattaagagg aatccgggtg
     3361 cgttcttatc aaaccatact cggctgggtc ggcgagcctc agttcgtgcg tatctgccct
     3421 gttcagaggt gtgactagcg tgacgtagtt tggaggaccg ggggagtatt tggagggttg
     3481 gtgccggggg tagcgttttt ctgagtcgtt aaccggactg ctcggcgtaa tttagaggat
     3541 ccccaaggaa gtgatcgacg tgcagcacca gggcgctctc gcgggagagg ccgtagcccc
     3601 cgtccacgac accggtcgcg gtgcgacccg agtcttggtc atgtccacgg cagccttcac
     3661 cgcgatgttc gcggtgtggc tgatgttcgg catcctcggc ccctcgatcc agaaggagtt
     3721 cggcctcacc gacgtcgaac tgtcctggat cacggcggtg gccgtgctca acgggtcgat
     3781 gtggcgcctg ccggccggga tgctcaccga ccgcatcggt ggccgcaagg tcaccgtctt
     3841 catgctcgcg gcgaccgcga tcccggccta cttcgtctcg acggcgagca gctacggcat
     3901 gctccttgtc ctggcgttcc tcgtcggctt cgccggcaac ctcttctcgg tcggcatcgc
     3961 gtggaacgcc gcgtggtacg gccgggagcg gcagggcttc gcgctcggcg tcttcggggc
     4021 cggcaacgtg ggggcctccg tcacgaagtt catcggcccc ccgatcatcc tcgggaccgc
     4081 cggtagttcc ttcgcgttcg ggctccaggg cgggtggcgg ctcgtgcccg tcatctactc
     4141 ggtgctgttg gtggtgttgg ccgtcctcac ctacatcgtc gtccctcggc gcgaccggat
     4201 gcccggggcc accaagcccg tgcgcgagat gctcacgccc ctcaagcagg tgcgggtctg
     4261 gcggttcagc ctctactacg tggccgtctt cggtgcctac gtcgccctct cggcgtggct
     4321 gccgaagtac tacatcgaca acttcggcgt cgagatctgg ccggcggccc tgctcaccgc
     4381 gacgttcatc ttcccggcct cgctcctgag gcccctcggt ggctgggtgt ccgaccgggt
     4441 cggcgctcgc cgggtgatgt attggacctt cggcgtcatg ctggtgacca caggaatcct
     4501 catgatgccg aacggccaca tcgtcgtcag tcaccctgac ggcagccaga ccacccacct
     4561 gggctaccag atcgggctgc cgaccttcgc gatcctcgtg ttcttcctcg gcgtggccat
     4621 ggggatcggc aaggccgcgg tctacaagca catccccgag tacttccccg acaacgtcgg
     4681 ctcggtcggc ggtctggtgg gcatgcttgg cggactcggc gggttcttcc tcccgccgat
     4741 gttcgcctac accaaggcgt ggaccggctt cccgtcgagc accttcgcct gcctgttcat
     4801 cgtcaccgcc atctgcgcgg tgtggatgca ctggaccgtc gtgcacatgt tgcacagcgg
     4861 ctcacccgag ctggccgacc acttcgacac cgagcacgtg agcacccgcg gctgatgcct
     4921 gtgctgcgag gtgtcacgcg gacggcatac ccccgcgaaa ctcgttatct gcgaactgaa
     4981 ctggagaacc gctcatgagc agcaccccga ccacccctcg ctcgggggag tggcttgacg
     5041 cttgggaccc agagaacgaa gccacctggg acaaggcgct cgcctggaag accctgtgga
     5101 tcacgacctt caccctcacc atggcgttcg tcgcgtggtt cctgccgagc gcgatcgtgc
     5161 ccaagctcaa cgcgttgggc tacaccttca gcaaggagca gctctactgg ctggccgcca
     5221 tcccgggcct gtccggcggc ctcatgcgca tcgtctggat gatcctcccg ccgatcatgg
     5281 gcacccgcag gatggtcgcg ctgacgaccg cgctgctctt cttcccggtc ctcggctggg
     5341 gcgtgcgggt gcagagcccg accgcgccct actgggagct gctcgtgctc gccttcctcg
     5401 cgggcatcgg cggcggggcg ttctcgggct tcatgccctc gacgtcctac ttcttcccgc
     5461 ggcgcctgca ggggacggcg ctcgggttgc aggcgggcat cggcaacttc ggtgtctcgc
     5521 tcgtgcagtt gatgacgccg tggctcatcg gcttcgggat ggtggggttc ctcggcgcgt
     5581 cccagcagat gaaggtgccc ggcaagcccg acgtgaccgt gtggtaccag aacgccgcgt
     5641 tcttctacat ccccttcatc atcgtcggca cgatcatcgc ctggacgatg ctcaagtcgg
     5701 tgccgatcaa ggccaacatc cgccagcagt tcgacatctt ccgcaccccg gacacctggt
     5761 ggatgacgct gctttacatc atgaccttcg ggacgttctc ggggttgtcg gcgcagttcg
     5821 gcctgctcat gaccaacctc tatggggcgg gcaatgcggc catcgtgtcc ggctcgggct
     5881 cgacggccac gctgctcgtc gccgggtatg ccgtgcccga cgccgtgaag tacgtcttcc
     5941 tcggcccgct cgtcggcgcc ggcgcgcgga tcctcttctc gccgctgacc gaccggatgg
     6001 gcggggcccg ctggaccctc gtctccggca tcggtctggt gggctcgatc atctacacga
     6061 tcggggggct ggtgcccgac acgtcttctg cggaggcgct gcaggccggc tttggccgct
     6121 tcctttgggg catgctcgcc atcttcctgt tcgcgggcat cggcaatgcg tcaacgttca
     6181 agcagatgcc gatgatcttc gagcggcgcc aagccggtgg cgtcatcggc tggaccggcg
     6241 ccattgcggc gttcgggccg ttcctcttcg gcgtcggcct gaccctcatg tcgccgaccg
     6301 ccttctactg gatcggcgct gccttcgcgg tgctgtgcat cgggatcacc tgggtccggt
     6361 acgccaggcc aggcgccccc aaggtgagct gaccggtcgg gttcgagccg tacgccggct
     6421 tgagcatgag gccgagttga gctgaggagg tatgccgcgc gtccaggggg acgcgcggca
     6481 tacctgcgtc tggggctgcc gcgcggcata cctccgcgtg cccccagccc accccccacc
     6541 acggagatag cgctcgcatg gtcatgtgtg ggggtgtttg aggcttgagt tgacggaaaa
     6601 gtggccatac gagcgctatc tccgtggtgg ggaggcgtgg ggtggggcgg accttgatcg
     6661 aatcttcgtc agacgtgcag ggggtcttga cgagggttcg agaggctgac agttatgagc
     6721 actctcgaca ccacatccgc caccactcat cgccgatccg cccttctcgc gcccgtcggc
     6781 gtgctcgccg tcgtcgctgg cgtcatcggg tggatgttgc ccgatcgcac cgtcggcacc
     6841 ggcgcgatgg atggcatgtc catgacccac tacatggggt tgcttgccgt caaccagccc
     6901 tggaacctca tcctcttcat ggccatcccg gtgatcctgg ccgaaacctt ggccatcacg
     6961 gagttggtgt tgctcttccg cagcgacccg cccacgtggg tgcgctcgct cagccggtgg
     7021 gccggactga tcgcaggacc ggtgatggtc ctcgtgctgg tgcacctgtt gaagaacgcc
     7081 gtcgtcccgc tcacgtccgg tggcggctgg cgcggcgcgg ccgacgtcat cgccgttctc
     7141 acctatctgc tggccggtct gcctctcatc ggcatcacgc tcgtcgaggt cggtgccatc
     7201 gggacggacg cgcgggatgc ccgcaagtgg cacgcggtct tcgtcggggt gttcctcgtc
     7261 ctggcccacg tcgcgatgat cttcgggatg ctcgatccca ccgtcctggg ctggggcgca
     7321 acgcacgaca tgcctggcgg cgggacgatg ccgggaatga accactgacg gcgttcgttg
     7381 ccgggtgaac caccgacggc atacccatgg ggggtatgct ggaggggttc tcacagaacc
     7441 ccgtgaagga gacccgaatg gccacgcaga gcttccaggt gaccgggatg acgtgcggcc
     7501 attgcgccgg tcacgtcacc gccgaactca agtcggtcgc aggcgtcagc gacgtcaccg
     7561 tcgacctggt cgccgggggc acctcgacgg ttcacgtcga cgccgaccat gagctgaccg
     7621 acgccgaggt caccgccgcg ctggacgaag ccggcaacta cgcgctggca ggccgctgag
     7681 cgccagtcgc tgacgtcccg tcggtgggcg cgccgacacc ggacgcgcca cccgcgagac
     7741 gaaggacaac cgaagagatg accgcaggac cggacaccca ggcacccccc tccgagctgc
     7801 agcagaccca actggtcgtc ggcggcatga cctgtgcctc gtgttcggcc cgggtcgaga
     7861 agaagctcgg caaactcacc ggggtcacgg cggcggtcaa ctacgccacc ggcaaggcgt
     7921 tcgtcgtgca tccgggcggc ctggccgtcg ccgatttggt ggctgccgtc gagtccgccg
     7981 gttacacggc aaccccggcg ccgccgtcga tcccgggctc ccaacccggg ccgggcgagg
     8041 gtggggagcc ggatgcggac gacgagcacg cggcatacct gcggcagcgg ctgctggtcg
     8101 cgctgggtct gagcgttccc gccattgcca tctcgatggt gccggcgctg gcgttcccgg
     8161 gatggccgtg ggtggtgctc gcgctcacgc tgcctgtcgt cacgtggtgt gcgtggccgt
     8221 tccatcgggc cgcctgggtc aacctgcgac acggcgcgac gacgatggac acgcttgtct
     8281 ccggtggtgt cctcgcagcg ttcgggtggt cgctgtggat gctgctgacc atgggggcgc
     8341 acgggcactt ctacttcgag accgcggcgg cgatcacgac gttcctgctg gccggccggt
     8401 atgccgaggc gaaggccaag cggcgctcgg gggcggcgtt gcgcgcgctg ctgcatctcg
     8461 gggccaagga tgtcgcggtg ctccgtggcg acgtcgaagt gcgaatcccc ttgggggagt
     8521 tgcttgtcgg tgacgagttc gtcgtcaggc ctggcgagaa ggtcgccacc gacggggtca
     8581 tcgtcgacgg agcctcggcg gtcgatgcct cgctgctgac cggagagtcg gtgccggtcg
     8641 aggtcggcgt gggtgatccg gtgaccgggg cgacggtgaa cacctcgggg cgcctgcgcg
     8701 tgcgggcgac ccgcgtcggc ggcgacactc aactggccca gatggcgcgc ctcgtcgagt
     8761 cggcgcagtc gggcaaggcc gccgtgcagc ggctcgctga tcgggtgtcg gccgtgttcg
     8821 tcccggtggt gttggtgttg gcggcactca cgctcatcgg gtggctggtg accgggcatt
     8881 ccgccgagtc ggccttcacc gcggcggtgg cggtgctcgt catcgcctgc ccctgcgcgc
     8941 tcgggctcgc aacgccgacc gcactgctcg tcggcacggg tcggggcgcg caactgggca
     9001 tcctcatcaa gggccccgag gtccttgagt ccacgcgtcg ggtcgacacc gtcgtcctgg
     9061 acaagacggg cacggtcacc accgggcgga tggccgtcac gggtgtgtat gccgctcccg
     9121 gccagtcccc ggagagggtg ctggcgctgg ccggcgcggt cgagtccggg tccgaacacc
     9181 ccatcgcccg cgccgtcacc tcggccgcgt ctgctcctgg attgcggttg agcgagttcc
     9241 gcaaccacgg cgggctcggg gtggagggcg tggtcgacgg tatgccggtc cgcgtcggcc
     9301 gcttgtcctt cgtcacctcc gcgcccgcaa ccgggcaggg ggtggacgtg agcctgagtg
     9361 aggctgccga ggaggcgatg gcccagggcc agaccccggt ctgggtcgnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnct gagtgaggct gccgaggagg cgatggccca
     9541 gggccagacc ccggtctggg tcggctggga cggaacggcc cagggtgtgg tcgtcgtcgc
     9601 cgacaccgtc aagccgtcat cagcccgcgc cgtcgagctc ttccgaggcc tgggcctgca
     9661 accggtgctg ctcaccggcg acaacgcgcg agcggcagcc gcggtggcac ggcataccgg
     9721 gatcgcgccg gaggatgtgc gcgcggaggt gctgccggcc gagaaggtgg ccgtcgtcgc
     9781 tgccctgcag gcgcagggca aggtcgtcgc gatggtcggg gacggcgtca acgatgcggc
     9841 cgcgctggct caggccgacc tcgggatcgc catcggcacc ggcagcgatg tggccatcga
     9901 ggcgtccgac ctcacgctgg tccggggcga cctggtcagc gccgtcgacg cggtgcggct
     9961 ggcccgggcg accctgcgga cgatcaaggg gaacctgttc tgggcgttcg cctacaacgt
    10021 ggctgccatc ccgttggccg cctccggtcg gctgaccccc atgatcgccg gcgcggcgat
    10081 ggccttctcg tcggtcttcg tcgtcaccaa cagccttcgg ctgcgccgct tcaagccgtc
    10141 tatctcggcc tagggtctcc cgcccggggc gggcgaggaa tagcctcgat gcatgacgat
    10201 cccctgcatc accgtcggtt ccggcgagca ccacgtcttc gtcctgcacg gctggttcgg
    10261 ctcggccgac gggtggggtc tcttcccgac ctacctcgac ggcgagcggt tcagctacca
    10321 cttcaccgac aaccgcggct atggcgcccg gatggacgag cgcggccact acagcctcga
    10381 cgaggtcgcc gacgatgtcc tggcgctggc ggattccctt gatgtgcaga cgttttcgtt
    10441 gatcgggcat tcgatgggtg gggccgaggt cctgcgcatc ctcgcgaagg cgccggggcg
    10501 cgtgcgcaag ctggtcggtc tgacccccgt cagcgcctcc ccgaccccgt tcgacgaggc
    10561 cgggcacgac ctgttctggg gcgccgcgga agacccgggc aagcggttcg ggatcatcga
    10621 cttcaccacc ggcaaccgca acacggccac ctgggtccag caggtcgtcg accactcgct
    10681 gagccactcg acgacggagg ggttcgccgg ggcgctcgag gcatgggccg gaccggattt
    10741 cctcgccgag atcgccggca cccagacccc gatgctcgtc atcgccggcg agcatgaccc
    10801 ggcgctcggc gaggcgacgg tccgtcagac gtgggagccg cacttccccc agtgcgagat
    10861 ccacgtcatg gccaatgccg gccactaccc gatgttcgag acgccggtcc aactcgcgac
    10921 cgtcatcgag cgcttcctcg ccgactgacc ccggcccgcc gcaggtgagc accgtcgatc
    10981 ggtcagccgc ccgcgcgcgg gtggacgcgg catacgcgcg gctcaccgag gtcgagcggg
    11041 gctggacgcg tgtcgtccac gaccccgcga cctacacccg agacgtcccg tatgccgccc
    11101 tcgcgggtct gcgcgaccga cacggtgtcg tcccggtcgc cgagccggcc ctgcccggct
    11161 ggccggaagg gacgggctac tggctggtgc tgcgccatgc cgacgtcgac cgggtgctca
    11221 aggatccggc gaccttctcg tcgtcgctcg gtggcaccca gatgcgcgat ccgcggaccg
    11281 aggacgacct ggcctacgtg cgccggatga tgctcaacat ggacccgccc gagcactccc
    11341 ggctgcggcg gctgttggca aagtcgttca cccccaaggc gatcggcgcg ctggaggctc
    11401 agatccaggg gcatgtcacg ggcatccttg accaggccct ggacagcacg ccgaccagca
    11461 ggcagggtgc gtgcgacttc gccaaggacg tgtcggccga cctgccgctg ctcaccctgg
    11521 ccgacgtcat gggggtgccg gcgccggacc gctggctgat gttcgactgg gccaaccggg
    11581 tcattggctg gcaggacccc gactaccgcg tctcggccgc cttcgacccg agcggggcga
    11641 ccgaactcgg gcgggcggca tacgcgctgc gcccctcgcc cggagcggac ggcctcatgc
    11701 ccgacccccg cacccgtgag ggcatgcctg acctctatgc ctacgcgcac gcgctcggga
    11761 cgctgcgccg cgagcagccc ggcgaggacg tcatgtcgat cctcatgagc caacgcgacg
    11821 acgccggcgg gtcggtgtcg gtggctgagt tcgagaacct cttctggttg ttcgccgtcg
    11881 cgggcaacga gacaacccgc aacggtatgc cgggtggcct gcttgggctg ctgggctcac
    11941 ctcaggcgca ggaggcgttg cgggcccggc cggacggcat accgaatgcc gttgaggaga
    12001 tgctgcggtg gtggacgccg gtgatgacct tccggaggac ggcgacgtgc gaggtcgagt
    12061 tgtccggggt cacgttgcag cgcggcgata aggtcgtcgt gtcgtttgcg gcggccaacc
    12121 gggacccgga ggtgttcgcc gaccccgacg tcttcgacat cgaacgggcc aacgcgcggc
    12181 ggcacctggc cttcggcgcg ggaccgcacg tctgccttgg ggcgttcctc gcccggtcga
    12241 tgatgacgac gatgttcact cagttgttgg ggcgcaccac ctggctggaa gcggccgggg
    12301 agccggtctg gttgcagtcc aacttccaac gcggggtcaa gcggttgccg atcacctggc
    12361 ggatgtgacg ggcctgactg ggctgggcac ccgctagatg agtggacatc agactggtct
    12421 gagactagtc tgaactacat gacgacgacc gtgggtgcct acgaggccaa gacccatctg
    12481 ccggcgctgc tcgcgcaggt cgagcggggc gagaccgtga ccatcactcg gcacggacgg
    12541 ccgatcgcca ggctcgtccc gatcgacgtc caccggccgg tggaggagat catccttgag
    12601 cttgaccggc ttcgtcgggg agtgaccacc ggcggcatga gtatccggga gatgatcgag
    12661 gaaggccgtc gctgatggcg ttcgtcctcg actcgtcggt ggcgctgtcg tggtgcttcg
    12721 aggacgaggt gactgaggcc acgttgcgga ttcgtgagcg gctatcgacc gagcgggccg
    12781 tcgttccgtc gctgtggctc ttggaggtcg ccaacgtgct acttctcggc gctcgccgtg
    12841 gtcgactcac ggctgaggcg gcagcagagt ttgccgacct gttggtgggg ttgccgatcg
    12901 atgtggttga accggagcgc gacatcgctg acctgatgca actggcggcc gagcaccggc
    12961 tcacggccta tgacgcggca tacctgcagg tcgcggcggc tcgccgactg ccgctggcca
    13021 ccctggacgc caccctggcc gaggccgccc gagcagcggg cgtcgacctg ctcggctgag
    13081 cagaaccgcc gccaggacgt cggaatcgct cacccgagcc acagatgggc tgcgccgccc
    13141 cggcgcggca tacagtgtgt cgccatgacc gacgtcacga ccgagcccgc cgctcccacc
    13201 accttccgtg tccccaccga ccaggcgacg gtccgcgcca acgaccgcca acacgtcttc
    13261 cactcgtggt cggcccaggg cgcgatcacc ccgctgccga tcgcgcgggc gcaggggagt
    13321 cggttctggg actacgacgg caaggactac ctggacttct cctcgggcct ggtccacgcc
    13381 aacatcggct acgggcaccc caaactcgtt gccgcagtgc aggagcaggc cgggcggatg
    13441 gccaccatcg cccctgggta tgccgtggac gtccggtccg aggccgctcg cctcatcgcg
    13501 agcaaggccc ccgacggact ggagatggtc ttcttcacca acggcggtgc cgaggccact
    13561 gagaacgcca tccggatggc tcggctgcac accgggcggc acaaggtctt cgcggcatac
    13621 cgctcctatc acggggccac cgcgggagcc ctgacgatga ccggcgaccc gcgccgcatc
    13681 ccgagcgagc cggggctccc ggggatcatc cacttctggg ggccctaccc ctaccgcagc
    13741 gcgttccact cgaccagccc cgagcaggag agcgagcggg cgctgggtca cctgcgcgac
    13801 atgatcatgg tcgaaggccc cgccgcggtg gccgcgatca tcctcgagac ggtcgtcggc
    13861 accaacggcg tgctcgtgcc gccgccgggt tatctcgccg gtgtgcgggc gatctgcgac
    13921 gagttcggca tcgtcatgat cgccgacgag gtgatggccg ggttcgcgcg ctgcggcgag
    13981 tggttcgcga tccagcactg gggcgtgacc cccgacctca tcaccttcgc caagggcgtc
    14041 aactccggct acgtcccgat cggcggggtc atcatcagcg aggccatcgc cgccacgttc
    14101 cgcgaccggg tctacccggg cggcctgacc tactccggcc acccgctcgc ctgcgcctcg
    14161 gcggtcgcct cgatccagat cttcgaggag gaaggcatcg tcgagcacgc ccggatgctc
    14221 ggcaccgacg tcatcggccc cgccctcacc gagctcgccc agcggcaccc cagcatcggc
    14281 gacgttcgtg ggctcggcgc gatgtgggcg gtcgagttgg tccgcaaccg tgagacccgc
    14341 gagccgctcg tgccctacaa cgccacgggg gccgcggcgg cgccgatgac ggagttcgca
    14401 gcggcctgca aggcacgcgg gttgtggccg tttgtccact tcaaccgcac ccacgtcgtg
    14461 ccgccctgca cgacgagtgt cgaggaggtc cgcgagggcc tggcgatcct cgacgaggcg
    14521 ttgacggtcg cggacggcta cgtcacgacc gactgagccg ggcgcccagt cgggcgccaa
    14581 atcgggcggg gcgtgcggca taccctcgcc ccgtgcggca ggatggggcg gtggccagca
    14641 gctcgggacg gcgcccggtg agcgtcgaag tggtcgagcc gcacatggcc gaccgtgttc
    14701 gccgcgcctc cgacgtctcc tggttcctcg tgtatgccgt cctcgcggcc ctgtcgatca
    14761 ccctcggcaa cctcgcggtc ggcaccgcgg gcgccctgga acaggacctc gcgctagcga
    14821 cggggggcct gccccggcta ctgctgcaac tgttctcgtg ggtcgccggc gtcggcgtgc
    14881 tcttcctccc gatcgccgtc ggcgtcgaca tgctgctgcg ctcgcgcggc tggcaactcg
    14941 tgcaggcgct gctcgccggt gggttggcag ccggcatcgc catgctcatc aaggcggcga
    15001 ttctcgacgg tcagttcacc caggtcctcg cggcgctcac tcgaccgctg aacagcaacg
    15061 ggcgcaccaa ccccctcgac gtcctcgtcg tgacgttggt cgcgctcgtc gtggtcgcca
    15121 acatcagcgg ccgacgatgg ctggcaaccc tggcgcccct cgtcatcggc tcgctgctgg
    15181 tcaccgggtt cctcgccggg tcgaacaccg ctctcgcgct cctgtgctcg ttcctgctgg
    15241 gcgccatggt cggccatgca ctccggttcg cgttcggcac cgcctcgacc cgggcgcccg
    15301 gcacggcgat cgcccgcgag ctggtcaatg ccgggactcc tctgcagacc cttgagttgg
    15361 tcgacgagta cgaagagggg gcccgcctct atcgcggcct gactcccgcc ggcgagatcg
    15421 acgttcatgt cttcgaccgg gacaccttcg ggctcgcctc cggccgccgc ctgctcaacc
    15481 ggctgcggct gcggggtgcc acggccagag cgccgtcctt caccctgcgc tcggccctgg
    15541 agcaccgcag tctgcaggcc ttggccttgc ggtgggccgg ggtctgggcg ccgttcccca
    15601 tcgcggtctg cgacgtcgac ggagccacgg cggtcctggc cctgacccgg gtcgaggggg
    15661 tctcgctgcg ggtcctcggc gacaacctca ccgacgccca ggctcgcgcg gtcgtgcggc
    15721 tgcttgtcgc tctgcaggag caccgcatcg ccttccgcgg gctcacccgg gacaacatcg
    15781 tcatccttcc cgacggggca gcaggcctgc tgtcggtcgg cgagggcgat gtcggcggcg
    15841 acgacatcgc caggcgagca gacgccgccc aggtcatggt catgctggcc ctggcgatcg
    15901 gtccggagcg cgccgtcgct ctcgccgtcg acgaggccgg gtcgcgtacc gtctctcgca
    15961 ccctgccgct gctgcaggcc ttggcgctcg gacgggagtt gcggggcgag ctcaaggctc
    16021 gcaagggtct gctcgacgag ttgcgtgagc aggtcctcac ccttgaggac agccacaacc
    16081 agcccgcgac catcgagttg cgtcgggtca ccgtcaaggg actgctcacc ctcgtgggtg
    16141 gcgcggtcgc ggcatacatc gtcctgccgc aactggccca ggtggatttc gccgaggtgt
    16201 tgtcccgcgc ccaatggccg tgggcgctgg ggagcgtgct ggcggccgtc ctgacgttca
    16261 tcggggcctc gctcgtgctc accggatcgg tgccgaccaa actgcggttc cggcacacct
    16321 tcatgaccca gctgtcggtg gcgttcagcg gtctggtggc gcccgccgcg atcggcaacc
    16381 tggctctcaa cacccgctat ctgcagcgtg ccggtgtccc ggcgggggct gccggtgcca
    16441 gcgtcgggtt ggcccaggtc ttccaattca ccagctacgt caccctgctc gccgtgaccg
    16501 gcgtggtcgc gggaatcagc ccgcaggcgt cgttccagcc gcccgccaag gtggtcgcgg
    16561 ctattcccgt cgttctcgtc atcgtcattg gcctcttcgc cgtgccgcgg gtgcgccggt
    16621 tcttccgcga acggctcctg ccgcaactgc gccaggtcct gccgcaggtg ctggccgtgc
    16681 tccagcgccc gcgcaagctc gccgaactcc tcggcggggc gctgttgctc gacatttcct
    16741 ttgtcgcggc cctctactgc gcgacctacg ctttcggcgc ggcgccaccc ctggcaggcg
    16801 tggccgtcgt ctacttcgcc ggcgcgatca tcggctcggc cgtcccgacc cccggcggcc
    16861 tcggcggtat cgaagccgcg atgtcctacg gcttgaccac cctcggagtc gacagcggca
    16921 cggcggtgtc atcggtcctg ctctaccgcc tggcgaccta ctggttgccc atccccttcg
    16981 ggtgggcatc aatcaaccac ctgaccaaga tcgacgcgat ctaacaccga ccggtgctca
    17041 ccccgcggcg gcggctgcct tctcctcgtc ggtcgcctcg cgcacgacga tccgcgtcca
    17101 ggccccgaca tagatccgat cgtcgtcgcc gagctcctgc ttgggcccga cgtcgattgg
    17161 gttttccgga agcgcgtcgc cagcgtcgcc gacgaacgtg ccgttcgagg acccgaggtc
    17221 ttcgacgaac caccgcgtgc catcagtcgt caactgagcg tgccgacggc tgacgccgct
    17281 gtcgctcgtg cagtcgatgt ccgggacgat gtgcttgctc gccgacactc ggccgatgag
    17341 cgcactgcgc acgagcagcg ggacgacgac cggcagcccg gcggagggca tcggctcgtc
    17401 ggcctcctgc acggcatacc agtcggggtc gatccacacc tcggcgaccc actccagcgc
    17461 gatggcggga ggcacgcccg ggcccgtcgc ggtcgnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnccgac tcgccctcgg gctccgagcc gcggggcatg gcgccggtcg
    17641 tgaagtcgta tccgcaggcc tcgcagaaca ggctctcggg gacgttttcg gtcgcgcagt
    17701 tggggcaggc aaggctggtg ggagcagccg gcgccgatgc ttcgggcgtg gcctcctggg
    17761 cggcctcagg caccagaccc tgggcggcgg cgtcgatctt ggccccgcac gtgtcgcaga
    17821 agtccgcggc ggtcgagtcg tggccgttgg ggcagacgta gctcacttct tgatcctcct
    17881 ggtcttggtg gaggcggtgt ccagcgccat ttcgtcgagc ttctcgacgc tcttgcgcag
    17941 ccgcacggtc ccagcctcgg cattgtcgat ctcgacgacc ttcttcagcc gcgaggtcat
    18001 ctcctcgtcg cccgtggccg tcgcgatctg caccgcccgg cccagcttgc gggtcgcgcc
    18061 cgcgtcgtcg ccggcggcct tcgcggccag cccgtcctgg atcgccgctg ccaactcggc
    18121 ctgaccggta tagcgcgcca cctggtcgtc gatccgagtc gtcagcgcct cgtcgttgga
    18181 ccaggtcgcc ttgaccagcg cctgggcctg cacctcgtcg cggacgacca actgcacccg
    18241 cgaggcgagc tgttcctggc cgaggctctt ggccggcagc cgcacggcca cgtgatagtc
    18301 gcgggtctcg tcggcccacg ctccggtcgg gaacgccgcc gtcaacgggt tgacctgcga
    18361 gcgcttcgcg gtgatgtcct cgaggttggg ggcgacctgc ttgacgaaca gcacctgcgc
    18421 gccctgaggt gcccagaccc gcaggtcgac cgaggcgacc ccgcgagcca tcgacgcccg
    18481 gatgagcttc tcgaactcgt cggccatccg ttcgggcgac gggatgaggt cgaccgttcc
    18541 gagcagcgcc gacgcgatcc ggcgggcctc gtcgatctgc caccggtcac cgagcccacg
    18601 cacgtcgcac tggaagaagc cggttgcgcc ctggatggcc caggtcaact ggtcgggggt
    18661 ctcgtgctcg ttggtgccgt cggtcagcag gatcgcgtgc cgctgcacca cgtgggggac
    18721 ggtgaggaag acctccttgg ccttggtcag ccaggtcccc atggcggtcc cgccgtcggc
    18781 gcggaactgt tggatcgagg caatcgcctc ctggcgggtc cgcgggtcca tccgcaccat
    18841 ctgggtcgcc ccgaaggcgg gatagaccag cgttcccacg tggttgcccg caatgatcgc
    18901 gaaccacgtg ccgtcggtga tctcgttgag ggcgacggtc gcggcatacc ggccctgcat
    18961 gatgccgttg gcgcccatcg agccggacgt gtcgacgatg aggatctcgg ccgcctcgcc
    19021 gccgccggtg ccgccaccga tgccggcctt tccggcgccg gagcaggtca ctcgcacgat
    19081 ggcgtggacg tcggtgccgc cgtccggcag gaactcgttc tggaagacct cagtggtgaa
    19141 ctgtgccatc ggggctgtcc tctcggtcgg caccggtgct cgatgcgggt gcgggtgcag
    19201 gtgcgggcaa ggatggggga gcgggctcgg cggtcggcgt ggccgacgcg gtgaccggct
    19261 cgcttgcagg gtatgccgcg ggagccggtt ccgcgtccgg cggcggttcc tgggtgctcg
    19321 ccggaggggc tgactcgggc ggcccggagg tgggaccgac ccgagccagc gccacggtga
    19381 tgttgtcgat gcccccttgg gcattggcga agtcgacgag cgcctgggcc agcgtcggcg
    19441 ggtgcaaccc cgcgctgccc agcccggcga cggtggtggc cacgaggtcg cgcaacgccg
    19501 ccgcgtccga gcagtagttc cacaacccgt ccgaacagag catcagccag ccgtcctcga
    19561 ccaactgcag cgaggcggtg tgcggggtga ggtcatccgg tgagtcgatg cccagccagc
    19621 gggtgatcgc gtgggcgttg gggccggtct cggcgtcctt gcgcggcata ccctcgcgca
    19681 tctgctcggc ggcgaacgag tcgtcctggc tgagttgacg cggctgggag gcgggagcgt
    19741 cgggcaacca atacgcccgg ctgtcaccga cgttgcccac gacggccagg ccctcatcga
    19801 tgagcgcggc cacgaaggtg cacgacggcg gggtctcgtc cgccgggttg tgggtcgctt
    19861 cgaggaccgc tgcccgggcc gcgacgacag cctcgcccag ccgcttgctg atggtcccga
    19921 cgacggcctc aggcaggccc acgccacgtg gcaccgggtc gtcgaggacc tcgcggccgg
    19981 cgcgggcagc ggcgaggctg gcgatgtgcg agtcggtcgt gttggacacc ccgtcgcaca
    20041 cgacgagcac ggcctgcgtc agcggacgag ccgaccccat gagggccatc gcgtcctcgt
    20101 tgcgcgagtg gcggcgcccg atgtcgcaca ccccgccgat ccaggcgacc ggctcttcgg
    20161 tgaagtggtc gcgcggatcg ggtgccttgg ctccgcacgt gtcgcagtag ccgtcgagaa
    20221 agctccccgt cccacactgg gcgcaccgcg gagccgcggg tgggtcgacc tcggccgccg
    20281 cgccccggga caccggcccg gtccagccga ggtcgagggg cgattcctca cctgcggccg
    20341 cggtctgggc ccgcaccggc gcgtgccggg tctgcgactc gccggtcgtg ccgacggggg
    20401 ccgcagcggg tgtcgaggtg tcggcgccga aatcgtgccc gcactcctcg cagaaggccg
    20461 cgtcggcggc ccgctcggcg ccgcaggccg ggcaggtacc cgctgcggcc ggcggtgcgg
    20521 cgggagcgac gggcgcgctc acaggacgct ccacggccgg acggcgttgg cttggtcgac
    20581 gagggcggtc cgcgccgccg ggtcaggggt cacctcggcg agttgccggt atgccgcctc
    20641 gagtccctgc cgcacgccgc tcacgctcgc ggggtgcccg gcgagggtga tgccggggtt
    20701 cggcccgccc tgcggcgagg tcacccaggt cagggcgttc tccaggacct gcacggtgag
    20761 cccggccttg tcgctcgcgt cgagcggcac gccgtcgacg ctgcgcagcg cctcggcgag
    20821 ggcgggcaac ccctggccgg agtcggcgag caactgacca cgctgatggc gcgccaaggc
    20881 ataggcccgt gacgtcaccg ggatgaggtc gagggcttcg accgcagccg gtatgccgcc
    20941 ctcggcccga cggatgcggg ccatgccgaa cgcgccgacc ggggtgtagt tgctatcggt
    21001 ccgggcgcac acggcataca gggactcggc gatcgccgcc tccccgctga cctcacaggc
    21061 gaaggcgagt gcgagtttgg gggcgagctc cccggggacc tggccgtaga cggcgttgaa
    21121 ggcggcctgg gcggcagcag tgtcgcggcg ggcgagagcc gccaagcctt ggatccagac
    21181 ggcccgccac tcccaggggt cttcgtgcag aagggtgttg gtggccgcgt cgacgactcc
    21241 ggcgtcgccg cgatcgagcg ctgtccgggc caactcgagc tgaacgtcgg gcgtggccgg
    21301 gtcagcggcg cgcaagggct cgaagcgctc atgcggctcg agggaccgga cgtcgcggac
    21361 gagcgcggcc tgggcgtcgt gcgggtccgg gcggagcctg ggaagggacc accactcgag
    21421 ccggtcggtc gagatcgagg gctcctcgaa caacagcgac gtggccgagg tcgtcgccct
    21481 accgaccttg gcgtcctgcg cgacggcctc ccgcaagacc ccgagcatct gcacccgcaa
    21541 ctcgtcggcg ctggcgaacc ggtcgtcggg cgacttggcg cacgccttga gcagcaagcg
    21601 gtagaacgag tcgtaacggg cgaagacggg ggtgtcctcg accggtggca gcgaatcgac
    21661 gtaggtcgtc tgatagcccc ggaactcggt tgtcaggacg acgaggctgc gcccgatggt
    21721 gtagatatcc gacgccaccg agacgccctg ttcagccacc tcgggggcct gatagcccag
    21781 cgtgccgaag atcgccgagt cgtcgtcgtc catccgccgg accccgccga ggtcaatgag
    21841 cttgaggtcg tcgccgacct ggatgacgtt gtcgggcttg aagtcgcagt aggccagccc
    21901 caggtcatgg aggtactgga aggcggggag gatctcgagg atgtaggcca acgcctggtc
    21961 gaccgggagg gggtcgtagc ggcccgtctt gcgcatccgc tgcttgagca acgccttgag
    22021 ggaggtcccg ccgacgtact ccatgacgat gtagccggcg ccctcgtgca tgacgaagtt
    22081 gtagatctcg acgatccggg ggtgctcgac ctgggcgagg aagcgctgct cggcaatggc
    22141 ggccgccagg gcgtcagggt cgccggtgtt gagcaggccc ttgaggacca cccagcggtt
    22201 ggagacgttg cggtcgcggg cgagatagat ccagcccaga ccaccgtggg cgaggcagcc
    22261 ggcgacgtcg tactgtccgg ccacgaggtc cccgggctgg agcttggggt cgaaggagaa
    22321 ggcctggcga cacttggggc agaaaccggt cgtgcggccg gggcgatcac cctggctgcg
    22381 cccgaccttg gtgccgcagt tggagcagaa ccgcttgtcc tcggggacca cgggattctg
    22441 catcaccgcc gtcgccgcat cgatcgccgg catcggcggg atgacggtca ggccagcgcc
    22501 cagacgggcc gagcgcatcc gctggctcga cgacccgact cgacgggtgg ccttggtgcc
    22561 ggtctggcgg gcccgggccg agccgatcgc cgtcgaggac aaccggttac tcgccaccga
    22621 acgggtcgag cccatcgggg acgggtcggc ggccgactcg gcagggtcgc tgtcggcggc
    22681 cgtcccgcag acgtcgcagt agccgtcgag gatgtgtcct tggcagcccg gctgggcgca
    22741 cggagtgccg ttggggacgg ccgaggcggc ctggggctgg ggccgggccg cgggtgtgat
    22801 cggctcttcc tcggcagcaa gggccgcagc tgccgctccg gctgccgccg cttctgggct
    22861 ggccaccccg gtgctggctg ccccggcgct ggtcgcccca ggcgtgccgc acacgtcgca
    22921 gtaaccatcg aggatcgagc cggtgcagcc gggctcctgg cacttcgcag gggtggtcat
    22981 cgcggcgatg ctcccttccg tccagaggac gtgtcggtgg tgcgcaggga catcgcgtag
    23041 gcctgatagg cgtcccgcaa cgcccgcgcg cggcgcaggt cggtgggagt ggtgtcgacg
    23101 acggtctgaa gtcgctcacg caggtggtcc aggtcggggt cgctcacgcc cgtggcacgg
    23161 gcctgcgcgg ccagcgcacc ggcatacccc gcgatctcgt cgcgttccgc gagcggatct
    23221 ccgtatgccg tgtgcacgac ggtgagtgcg cggcccaccg cgtcgaggcg tttgaggtag
    23281 gtgtcgaccg cggcgccgcc ggagggaacc ggcccgaggg cacgcacgtc ggggaccgcc
    23341 agccggggag cgggcgtgac cgccgcgacg catcggtcga cgagttgctg gacggctgcg
    23401 ctgcgggcga ggagttcgtc gcgcacctgg gcggcccgct cggcatcccg ggcggtctcg
    23461 acccgacgtc cggcgccgac gatgaggtcg cgttcggcgc ccgcagcctc caactccagg
    23521 ggcgcgagga gtccgcccac ctcggctccg cgcttggccc ggtcggtgac gtcgacgagg
    23581 cgctgttcga gacgggtcag cagcgcgcgg gccgcctcgc ggtcgactcc ggcaggggcg
    23641 atggcgacct ggtcgcggat ccgttcgatg gtggcccgga cgtccttgag ccgggtgccg
    23701 acctcggcgc cggacggctc aagagacagt cgcgcacgca acgacgaggt catcgcgtcg
    23761 cagagccggc acgcctcggg caacgacagg cccagggccc cggccgtggt gggggagatc
    23821 gtcgcggccg ccgagtcagg cgccacgtcg aggcgacccc agatgagggt cgagagtcga
    23881 gccgcttcgg tggggcccac ccggccggag tcgaaggtga ccgtcagcag gtcgaaacgg
    23941 tcggagatgg ccttccacaa cgccatcgac acgagcatgt cgcggctgaa ggaatcgcgg
    24001 tcggtggagg ccaacgcggc ggcgtcgagc gagtcgagct cggccttgcg cttgtcccgc
    24061 cacaccccga ggtcgcgcag gtaggcgtag gcctcgtgga cccccaacgg ggtgccgagg
    24121 cgcccgggag cctccggagc cgcggcaccg gggagcgccc cgccggcagc cggctgggga
    24181 atcgggtcaa gggacaggct cacggggcgg tcaccgtcac tgacgcccgt agaccgcagt
    24241 cggcggcgtg ggggcgtcac cgagggacgg ggcgagccac cggtcgtagc tggccttcca
    24301 ccgaccgtcg gccttcatgg tctccaggag ccggttgacg tattggacga acaccttgtc
    24361 ctcggagttg aaacccacac cgtagggctc ggcggtgatc gccgtctggg ccgggacgac
    24421 ggcatacggg tcctgggtgg cgagccccgc caggacggtg tcgtcgccgg tgatcgcgtc
    24481 gaccttggcc tgctggaagt gcaggaggca cccggtgtgg gtgtcggcgg tgaccgggac
    24541 gacgcccttg gccacgaact cctgcagctt ggccaggctc gtcgtgccgg tgggagcgca
    24601 caccttcgcc ttggcggcgg cgagatcgtc caggctggtc gccgtgctgc ccttacggac
    24661 gagcaccttc tgaccggagt ggtagtactc cgcagagaag gcgatcggcc agtcgagcca
    24721 ccgagcgcag ttcatcgtca tgttgcgcac gacaatgtcg accgtgcgat tgacgaggaa
    24781 gctctgtcgc tgccccgccg tgatgacgac gagctgcacc ttggcggcgt cgccgaggat
    24841 cgccttggcg atctcgtgga cgaggtcgat gtcgaagccc tcgatctggc cggtcagtgg
    24901 gttgcgggcg cccatgagca gggtgtcggc cgacactcca gcgatgaggt agccacgggc
    24961 ccggatctcc gcgagccgcc cggtgagagc gccagaggcg ggaagcgagg gcaacgggtc
    25021 gtaggtctgc agcggattga accccgcggg caccggattg cactttgagg cagccggggt
    25081 cggggccgcg gtcgtcggcg cggcactcgt ggtggcggcc gcggtcggcg tcgggctcag
    25141 cggggtctgg gcatacccac cggccccgca ggcggcgagc gagagcagca gggtcgtccc
    25201 ggccgcccac agcggcaggc gggtgcgcgg catacggctc atcggtactc ctccacacgc
    25261 tgggcgatcc ctcgggtcga gagcaccgcg gcagccaggc cggcgagcag caggacgaga
    25321 gcggcgatgc gcgccttggt ggtcggtcct tcgatgtcac cagtgagggc gctctggaac
    25381 ccgtcgacgg cggtggcgct cgccgcttgg aaggcgtcga aggtggtccg gggcgactcc
    25441 gtcgcggtgg tcgccttggc cacggctgcg tcccagctgc ccccgtcgtc cagggtgcgc
    25501 acctcggcgt gcgccttctc gtatgccgac caggcggtgc tcagcgcgct ggcgttggtg
    25561 cctgacccgc cgtaccaact gacccggccc acgagggcct tcaatcgggc ggtcacctcg
    25621 gcgtcgcggg ccttccacag ggtttcgaag ggtcctgcct ggccgcggcg gatcagcccg
    25681 aggctttcgt tggcccgggc gtcgaaggcg gcggtccggg cgctggtgag ggccaaggtt
    25741 ccggcgagct ggttgtcggc cacgcgcttg gcgtccgaac cgatgccgtt gaacacgacc
    25801 aacccggaga tgagcgcgac gagaacggcg acggtcgctc cggccagccc ggagttgaga
    25861 tagcggtggg tgcgccgggc cagccagatg ctgacgagcc cgaggacgag cacggcgagg
    25921 acgccggcga cgagggtcgg ccaggacgag ctggctgcgc caagctcacc gcgcgagcgc
    25981 tcggtgttgg cctgcagcag ggcctccagg atcggcaccg ccgtggtgcg caaaccggag
    26041 cttgccgagg tcatgtaggt cacgccgatg ggcagcccct ggcggttctc cgcgcgggcc
    26101 cgttcgacga gagcggcgta gtcctgcagg gcactgttga gggccgcaag cgcgacaccg
    26161 tcggccggct gggcgctcgc cgccgcggtg atcgtcgtgg cagcccgctt gatcgccgcc
    26221 tcgtagtcgg cgcgttgggc cgggtcctcc aggccgccga cgaggaacgc gttgctcgcg
    26281 tcggcgtcag cacgcagcac gtcggcatag atcgactgga cccgcacgac ctggtcggtg
    26341 gttgccccgg cccgcccgag cgcctgggcg ctggaccaca ggttccaggc ccccgccagc
    26401 ccgaagacaa tcgcggcgat ggcggcgagc gcaccgaaca ggcgcatccg gccgggagtg
    26461 ccgctggcct tcccacgcac ccacgtgccc acgcgcgcct tggccggtgc ccccgccgag
    26521 ttcgcgtatg ccggtcgcac gcctcccgct gccggagcgg gcatggcgac ggcaggagca
    26581 actgtctcgg cgccggggcc gctcgcggaa ggaggtggcg gtgcaacggc ggccgctgcc
    26641 gcggcaggcc cgcgggatcg cgcctgaggg ttttggctca tgagggggtg gctccttcgt
    26701 ccgtggagtc ctcgggtggt tcggagctct catctgcggg gtcgtccccg aaggcgacga
    26761 tgtcggtcgt ggccggctcg ggatcgacgt gtgggcggaa gtcctcgacc cgcagctcgc
    26821 gcaattggtc ggaggtgagg tcggcgtcct gatgcaaccg ccaggcctgt cgcccgatgg
    26881 cttcctccaa ggcgttgcgg gcgaagcgcc cgttgccgaa cgcttcgttg cgcgccgtcg
    26941 cggccaggac cgaccggaaa tgcccttccg cctcgggcgt caccgtgtag tcggccgcag
    27001 tggcgagttt gtgcaggatc gcgacgatct cgtcgtcggt gtagtcctcg aactcgatga
    27061 ccgtcttgaa ccggctggac agcccggggt tctgggcgat gaaggcgacc atcgggtcgg
    27121 gatagccggc gacgatgacg acgaggtcat cgcggtggtc ctccatctcc ttgacgaggg
    27181 tgttgaccgc ctcctggccg tattggtcac cggacaaggc gtaggcctcg tcgatgaaca
    27241 agacgccgcc ataggccttg gcgcacgtct cggcggtctt gatcgccgtc tggccgaggt
    27301 agcccgcaac caactcggag cggtcgacct cgacaagctg ccccttggac agcagaccca
    27361 gggattgata gatcccgccg accatccggg ccacggtcgt cttgccggtg cccgggttgc
    27421 cgacgaacac caggtggcgg gtgatgtcgg cgttcttcag gccggcttcg cggcgcagtt
    27481 tgtcgacctt gagcatggcg acctgttggt gcacctcgcg cttgacccgc tcgaggccga
    27541 tcatcgcgtc gaggtccgcg agaagctcct cgagggtctt ggtcggcttc ggcggctcgg
    27601 gttcggcggg cggcgccgca tcgacggggt cgccggccac ctgggcctgg tcaccgcccg
    27661 aagatggctg gctaggcgtc cctggtgtca ccggtgtccc tggtatgccg ttcggcgtcg
    27721 accacgggaa aggcgtcagc gtcggggtgg gccagatccg gtcccactcg gaggtggggg
    27781 agggcggcat accggggacg cctgggggca ggccccggga ggtcgcgccg gggtcggcca
    27841 ggcgggcggc ctccagttgg gcggcggcgg cgacggcggc attgcccgtg acccgcaacg
    27901 tcggttcgcc gagggagcag gccgccgagg cgacctcggt gagcgccttg gcgtatgccg
    27961 tcgcgcgccc gggagcggtg gcggcgagct cgctgagcaa ggccgtcggc gcaccgcggg
    28021 acttgcggcc gcggctggcc gcgtcgaaga aggcggcgtt gaccgcagcc aactcggtcg
    28081 gggcgccctc gatggcagcg caccagtcct gggccgcgcg gggagccgct tcggcgatcg
    28141 cggcagcgaa gaccatcgcc tcgtcgcgag cccggtcagg cggcaggccc gcctcggtgg
    28201 cgacggcgac gagggcgtcg accgcctcac gcagctggct catctagccc tgccttccga
    28261 tggggggttg caaccggact tgtggggtgc cggtggcggg cgtctgcgtc gcagggggcg
    28321 tcggtgccgt gggcgtctga gtcgcagtgg gcgtcggagc cggcggcatg gtcggcgtgg
    28381 tgggcatcgt cggcagcggc gtcgcggacc cgagggccag ctcgcctccg gtcgacgcgt
    28441 gcccgaactt ctcctgcagg aagcggccgt ggatgaccaa ggcctcggcg tcggcgcggt
    28501 tgatggcgtc gaagacccgg tccatcgtcc ggccgagcag gtcgagctgg tcgacgagga
    28561 tcatcagcgg ggtcttgccg cggtcgaccg gccgggagtc ggcccactcg cggggcagcc
    28621 ggaggtagcc cccgatcgct tcggggaggt aatcggtcgc cgtcgccatg acgtgatacg
    28681 cctgggtgct gcccccgccc agccggtcca tccgggggat cgtctcgcgg accgtcttgg
    28741 tcacccggcg cagccgggcc agcacctcag ggcggacctt gccggcgacg gccatggctt
    28801 cgacacggtc cacagccgcc aacaggtcat cggccgtcgg cacacgcggg atcggcgtga
    28861 ccggcgcctg ctcctcgcgg cccccgaaga gccattcgcc gatgcccatg gttggtgatt
    28921 gtcccctgtc ccgtctgctc tgtctgtcgt ggacttccct ggccgagccc tgacccgacc
    28981 ctgacccgcc tggccgcagc ggtgagcgtc agcggcccag gtcgagcgag cccatctgga
    29041 cgttggggtt gacccggttg gcgcgctcga gataggtccg cgacttggcg acctcggtct
    29101 caagcgtgcc gatcgtggcc gccatgttgt ccagggcctg caccttgaac gtgtcgatgg
    29161 cgtccatggt ctggtagatg ttcgagaacg ccgcctgcaa ctgctcgatc ccgatcgtgg
    29221 cgctggccgc ctgctcctgg atgcgcaccg agttgtcacg caacatctcg gaggtcgact
    29281 ggatgagccc cgaggtcgtc gtgttgaggg cggtgatctg gtcgaggacg agcttctggt
    29341 tgccgagggc ctgcgccacg atgacggcgg tgcgcagcgc cgacaccgtc gtcgtggagg
    29401 cccggtcgac gcccttgacg agctcgatgt tgttcttgat ggtgatgtcg atggcgaggt
    29461 agttctggat cgacacggcc aactgggtga gcaggtcctg gtgcttctgg cggacataga
    29521 agagcacgtc ctgcttgagg gcggtggcct tctcggggtc ggtgacgtcc aaggtggcga
    29581 tctgggctgc catcttggcg tcgaggcgct cggcgatgta gacgtactgg ttgagccggc
    29641 ccatcgagtc ccagaggttc tgcttttcca ggttgagcgc cacgttgtcg cgctgcaact
    29701 cgtcctggcc ctggcgtagg gcctgcagga tgccgtcgag gtggctctgg gccgactgat
    29761 acttgcggaa gtagtcggtg accttgtcgc cgaatgggat catcccgagg accttcttga
    29821 cacccgtcgc ctggctgggg tcgagatcct cgacggtgcg gcgcagttcc agcagggtct
    29881 tgccgacctt gccgccctcg gacaatccgc cctcctgcag cgccttgacc ggcgaccgga
    29941 gcagtcggtt gctcgactcg gcggcccggc ggatgtcctc gtcgcccatg acgcgcacgt
    30001 cgttggcctt ggtctcgaac tgcggcgact tcggggccgc ggtcatcaac gcctgcaagt
    30061 agttctcgac cttggcgtcc aggccgggca gggccgcctc agggacggcc ggggccatcc
    30121 gcggcgccgc ggtagtcgag atgggtgcgg cggcggccgg gggagcctcc agacgcagcg
    30181 agtcgttggc cgcgggcggt gccagcggct gcactgccgc ggcgttgctg gcggaggcgt
    30241 cctgcgcgcc gtaggtcggc tggctcatct gtctccctgt cctggagcgg gtatgccgtg
    30301 gtccggctgg tcgatcgggg gtcttggggc gtttcggtca ccgcagcaac aagagcggtg
    30361 ccgatagtcc agacgtgcgg ccaatctacg cggttcccca ggggtcgtgc aggggagccc
    30421 ggagccgtcg cgcaggcttt cggccgggcc accgaagcgc gatggtcacc ggtgcgcgac
    30481 ggcggaaccg agcatgtatg ccgcgacgag gaacaagagc atcgcgaagc cacggcgcag
    30541 tgtctcgggc gacgcgcggc gagctagtcg tccgccgatg agaccgccga cgatggcccc
    30601 gagcgtgaag gtcccgacga gcggccaatc cagcgacacg ccctggatca ctcgggcgcc
    30661 gagtgcggtg gcgctgttga gggcgatgac cagcagcgac gtgccgatgg cggcgcgcat
    30721 gggatagccg aggacgagcg tgagggccgg gacgatggcg aacccgccgc cgacgccgaa
    30781 aaagccggtg agcagcccga cgccgatccc gaccgcagcc accgggccga agcgccgaac
    30841 ccggtgtccg ccggcccggt cgtcagcagg gggcggtcga tgtcggccgc ccaccgcccc
    30901 gtcatcagcc ccgtcggcga ccccctcggc cgccccatcg atgagactgc cgaggtcctc
    30961 gatgacggcg gaggactggc gcaacatgag cgcccccacg acggccaaca ggctggccag
    31021 gaggacgacg aggaccggcg cggccaccgc ggctgaggcg agcgacccgg catacgtgcc
    31081 gatcgtcccg acggcggcga ggatcaccgc ctcgggcact cgggcggtgc cgccgcggat
    31141 atggctgatc gccgtggcgg ccgcgctcac gccgatgatg accagcgacg cggtcgtcgc
    31201 atcgtgcggc ggcataccga cgacgtagat gaggacgggg atggtgagga tcgcgccgcc
    31261 cccgccgagc gcccccatga ccgcgcccgt gagcaggccg agtgggacgg cgatccagag
    31321 caccgggcca ctctagagcc aaccgat
//
LOCUS       DAORPW010000011        30650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_218_length_30650_cov_503.824285, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000011 DAORPW010000000
VERSION     DAORPW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 30650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30650
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218_length_30650_cov_503.824285"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PLB86_02010"
     CDS             complement(<1..1001)
                     /locus_tag="PLB86_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRC63943.1"
                     /translation="MAQDGSLELLRLLADSAPTHHLDRIGAQPEAHRLARAVALRAED
                     QRRREGQLRALVDTARDLAAARDPSGVLDAIVRRARALMGTDVSYLTLYDPERGDTYM
                     RATDGAVAPTFRALRLELGVGLGGLVASSLSPWWSTDYPHDDRFAHTTTIDTGVQDEG
                     LVAICGTPLVAEGEFVGVLFAAHRTRHTFTQEEVALLGSLADLAAVSMVQARAHQETA
                     RALAALSVAHEAVRRHTEVIERSAAAHDRFTELIGAGGGVTDLTTALVGLVGGWAVLT
                     DAAGTRRSAHGTPPGDPVEAGAVDPLADTPLARAARASGGLTVEDMRYAVPVRAGHH"
     gene            complement(1048..1908)
                     /locus_tag="PLB86_02015"
     CDS             complement(1048..1908)
                     /locus_tag="PLB86_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC63944.1"
                     /translation="MTTHPMTGRVVLITGAAGGLGRAHAHAFAALGAHVVLSDVTDPA
                     AVAAEVTAAGGSALGVVAGVDTWEGAAVSVAAAVEAFGRLDVLVNNAGIIRDKSFAKA
                     TPEMIEAVLAVHLHGAFHMTKAAWPHLTERGGSIINTTSGSGLYGNFGQVNYAAAKAG
                     LVGFTRSLALEGARAGIRANAIAPLAKSAMTETMMAPELLERLAPEWVSPLVTWLASD
                     GCTATGNVYSVGAGRYARVATLEAPGVRFDHVPTIAELTEAAAAIDDLAGAVEPRSLA
                     DQIALVAPPA"
     gene            complement(1905..3566)
                     /locus_tag="PLB86_02020"
     CDS             complement(1905..3566)
                     /locus_tag="PLB86_02020"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRC63945.1"
                     /translation="MTPIASPAPGSAAPGSVASGPHTSLVRSVESAMELIRSGETIAT
                     SGFVGCGTPDALLDGLARRFRESGDPAGLTLLFAAGQGDGGEQGLNRLAEPGLLRRVV
                     GGHWGLIPRVAALAVSGQIEGWNLPQGVISRLYRDMAAGLPGTVSRIGLHTFVDPRSQ
                     GGRVNERTTDDLVEVVHLGGEELLFYRATPIDVALLRGTSADEHGNITMEREALTLDN
                     LAMACAARNNGGKVIVQVERIVSAGTLDPRLVKIPHILVDAVVVAPPELHRQTYATAF
                     NPGYAHEVRIPLDLIPPMPMNERKIAARRAAMELPDGGVVNLGIGMPDGVSTVAAEEH
                     VIDRITLTTEPGIVGGMPASGGDFGAALNPDALIDQNQQFDFYQGGGLDLTVLGMAEL
                     DADGNVNVSRFGPRLTGAGGFIDISQSARTVVFVGTFTAGGMQVGISDGRLTILSEGR
                     SRKLVRHVEQVTFNGRYAASLGQRVLYVTERAVFAATPEGLRLIEVAPGIDIERDVLG
                     QMDFAPIVQDVALMDSRIFLPQPMGLAALLRPRTTSTPTLEGVSA"
     gene            3565..3693
                     /locus_tag="PLB86_02025"
     CDS             3565..3693
                     /locus_tag="PLB86_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC63946.1"
                     /translation="MKADLRVDGWGAEGAGSGAARAQSITPAHSSGMWAAYMLDGR"
     gene            3777..5174
                     /locus_tag="PLB86_02030"
     CDS             3777..5174
                     /locus_tag="PLB86_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HRC63947.1"
                     /translation="MSFLIIIAALAFLMWMAYRGHSVILFAPIAALAAVLLTDPSLVA
                     PMFTGLFMDKMVGFIKNYFPVFLLGAIFGKVIEMSGFARAIVTWVIKVVGAKRAILSI
                     VLVCALLTYGGVSLFVVVFAVYPFAAEMFRMGDIPKRLIPGTIALGAFTFTMDALPGT
                     PQIQNIIPTTFFKTNAYAAPILGFVGAIFVFSLGMLYLERARRKALAKGEGYGEGHIN
                     EPEVFSGERKVNPALAVLPLVLVAVVNKVLTSKIPEWFGDKVSFSPTVVGKSDPVTVA
                     VSSNVAIWAVEGALLIGILTVIALAWKPVSSHFAHGSKEAVGGAMLAATNTASEYGFG
                     AVIAALPGFLVVANGLAKIPNTLVNEAVTVNVLAGVTGSASGGLSIALGAMANTFVEN
                     ATAAGIPMEVLHRVASMASGGMDTLPHNGAVITLLAVTGLTHKVSYKDIFAMTIIKTV
                     AVFVVIGFYYLTHLV"
     gene            complement(5246..6772)
                     /locus_tag="PLB86_02035"
     CDS             complement(5246..6772)
                     /locus_tag="PLB86_02035"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRC63948.1"
                     /translation="MSEDYRSRIGTLIRDARRHKGLTQTELGRLLGTSQSAVARIEQG
                     HQNLSLEMLARIGEALDRELVTLGNSRPQHLRIVGGAKLSGAIDVKTSKNAAVALLCA
                     TLLNRGRTTLRNLARIEEVNRIIEVLSSMGVKVRWLPDSNDLEIVPPATLDLEGIDED
                     AARRTRTVIMFLGPLLHDRAEFRLPYAGGCDLGTRTVEPHLAALRSFGLNVVATHGFY
                     EAHAAPHQPDKPIILTERGDTVTENVLLAAARNPGTTTIRNASPNYMVQDLCFFLLKL
                     GVQIEGIGSTTLKVTGVADIDVDVEYSPSEDPIEAMSLLAAAVVTESELTIRRVPIEF
                     MEIELATLEGMGMQVKMTPEYLSANGHTRLVDITTIPGPLKAPIDKIHPLPFPGLNID
                     NLPFFALIAAMAEGTTMLHDWVYDNRAIYLTELTKVGAKVQLLDPHRVLIEGPTKWRA
                     AEVMCPPALRPGVVVLLAMLAAPGTSVLRNIYVINRGYEDLADRLNNLGATIEVFRDI
                     "
     gene            complement(6880..9027)
                     /locus_tag="PLB86_02040"
     CDS             complement(6880..9027)
                     /locus_tag="PLB86_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRC63949.1"
                     /translation="MSETTTAAPVAAAVDFAALFPEEVVTKALVRDVTMPGQAGTMAL
                     VTLDNGFDHTKPNTFGPASIAGLQACFDGLRARAEAGEIQAVGLTGKPFIFAVGADLS
                     GVPKVTQREQALAIARAGHAAYATLMDLPVPTFAFINGAAMGGGVEIALACDYRTISA
                     GVPAVALPECFLGLVPGWGGCYLLPNLVGPAKALTMIVENALNTNRMLKGPAAFELGV
                     ADAMFAPAEFLEASIDWAAKVVSGAVTVTRPEIPRDEATWTGAIAASTMLADLKTGKQ
                     SPAPYRAMKLVAAARTATRDEAFAAEDEALADLLMGDELRAGLYAFDLVQKRAKRPAG
                     APDRSLARPVTKVGIVGAGLMASQLALLFIQRLEVPVVLTDLDQERVDKGVGYVHAQL
                     DGLAKKGRLNPDKLNRFKALVSGSTSKAGFADAEFVIEAVFEEMGVKKQVFAECEAVV
                     SDTCVLATNTSSLSISQMAADLAHPERVVGFHFFNPVAVMPLLEIIPGSTTDPATLAT
                     AFAVGAKLKKTCILVKDSPSFIVNRLLGRFMGEVGRVADAGTPVEVIDQAFMGFAPMP
                     PFVLLGLVGPAIALHNGETLIKAFPDRFYVSEGLKAIVEAKKPGFYTFAGGKPALDPE
                     VLALLPQPENPVVLTAPEVRERVLGALAQEARMMLDEGVAQAPMDIDLAMITGAGFQF
                     WNGGLTPLLDRSGVAEQVAEARFLPAGAASVPA"
     gene            complement(9031..10254)
                     /locus_tag="PLB86_02045"
     CDS             complement(9031..10254)
                     /locus_tag="PLB86_02045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRC63950.1"
                     /translation="MTRTLREVVFVEGVRTPYGKAGDKGIYAQTRADDLVIRCIRELL
                     ERHPELPKDKVEEVAIAATTQIGDQGLTLGRVAALLSGLPNTTPGYSIDRMCAGAMTA
                     VTAVAGSIAMGAIDVAIAGGVEHMGRHPMGEGVDPNPRIIAEKIVDPSALVMGQTAEN
                     LHDRFPQLTKARCDAFALGSQGKLAAAYAAGKIQPDLVSVATRHKELGYGLATVDEPP
                     RPSTTMADLEALKTPFRVRGNVTAGNSAGLNDGATACLLASEDAAVAMGMPVRMRLAS
                     FAFVGVEPEVMGIGPVPATEKALAKAGLTIEDIGLFELNEAFAVQVVAFLDHFGIADD
                     DPRVNEYGGAIAIGHPLASSGVRLMTQLARQFKEHPEVRYGLTAMCIGIGMGGAVIWE
                     NPHHADYVASASKEA"
     gene            complement(10370..11548)
                     /locus_tag="PLB86_02050"
     CDS             complement(10370..11548)
                     /locus_tag="PLB86_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRC63951.1"
                     /translation="MLFPADGVPEVVIDERQLMAAAAAIARGTGPVALDAERASGHRY
                     GQRAYLVQLRRAGSGTWLFDPAACPDLSPVAEAIGDAEWVLHAATQDLACLAEVGLRP
                     QRLFDTELGGRLAGLPRVGLGAAVEHYLGLSLAKEHSAVDWSRRPLPEPWLRYAALDV
                     EVLVDLRDAIAADLTEQGKWEWAAQEFDSLLGFTGPAPRVDPWRRTSGMHKIRRRRGV
                     AVVRELWYVRDRIAQRRDVSPGRVLSDAALVALAVEPPKDAGAFAARRELRGVARSPK
                     VWLDAIDKALALSEDALPPLTLSSTGPPPPRLWADRDPIAAARLGRARDAMAAYASER
                     VVPVENLLTPDLLRRVLWTPPDDLGESAFAAALRGGGAREWQVNVVAPILATAAAEHP
                     "
     assembly_gap    11635..11734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11829..12410)
                     /locus_tag="PLB86_02055"
     CDS             complement(11829..12410)
                     /locus_tag="PLB86_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HRC63952.1"
                     /translation="MRGRSLPGDTAAEFTRAIAGLREARLRPEVRLTEVPAPQRIAPY
                     AVALSGEVVGPGGEEELASGRFVLLHDPSCPEPWEGAWRAVTFARAELEPEVGTDPSL
                     GEVGWAWLIEALQARGLRYCAEAGTVTRVVSERFGGLADQLPTVEIEVRASWTPLGGD
                     LGDHLLAWSDLLSTIAGLPPLPDGVVALPGARR"
     gene            12493..13332
                     /locus_tag="PLB86_02060"
     CDS             12493..13332
                     /locus_tag="PLB86_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC63953.1"
                     /translation="MPALLALLSAAVWGASDFAAGVISRTLRPVVVVAWSQSMALVLL
                     TAIVAFRGLPEQGFAGWFPWAMLAGVSGAMGLLCFYAALSTGTMGVVAPIAAVGSGIP
                     VLLGVLSGEKPSALAWWGIAVAIAGVVLASGPEMKEGLGARPVLLACLAAVGFGVALF
                     ALDRGARVSLLHTLWGMRITSAIGYAALALAARSLGGVARRHVPVLAAVGAGDLAANA
                     LFGMASSMGMVSVAAVLGSLYPVMTILLARYFLNERLRRIQVIGSTLALCGVVLIAAG
                     GRG"
     gene            complement(13361..14437)
                     /locus_tag="PLB86_02065"
     CDS             complement(13361..14437)
                     /locus_tag="PLB86_02065"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRC63954.1"
                     /translation="MFSYAVSRRLRPAALTVAALLALGACSSGSSPTSTNAAREGGVA
                     VAPAPAPGAAVGAAAAGDTKLSAPDVASVGEAALVDTKIIRTASIYLTVTSVETAAAA
                     VRGVAAGLGGAVASETVNATEVAPSADPAIGGKATAPVRRVGNYGQLVLSIPTDRLDA
                     ALDQLSKLGTVVQRASASDDVTAAYVDTESRIATKKASIERVRALMSQTQNISQIVEL
                     ESQLAQREADLESLQAQLASMTKRIAMATVTVTVSSNPEVIPPVEQDGFLAGLRSGWN
                     AFLASLTVGLTVLGAALPWLIVLALIAIPVRSWLRRRSERQEATPTTPPSWAASTPAA
                     PVREPEPAAATAGPAASEGTSEKA"
     gene            complement(14547..15011)
                     /gene="msrB"
                     /locus_tag="PLB86_02070"
     CDS             complement(14547..15011)
                     /gene="msrB"
                     /locus_tag="PLB86_02070"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRC63955.1"
                     /translation="MTDPTSTQSVPPADAVPTPGRYAVQLTDTQWRAKLSAAEFHVLR
                     EAGTERPFTGEYTDTKTDGVYSCRACGAELFRSESKFDSHCGWPSFFEPLAGDRVEYI
                     EDRSIPGRPRVEVRCAGCGSHLGHVFEGEGYGTPTDQRYCINSVCLTLEPRT"
     gene            complement(15079..17502)
                     /locus_tag="PLB86_02075"
     CDS             complement(15079..17502)
                     /locus_tag="PLB86_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRC63956.1"
                     /translation="MSHVWTHVRRPAVTLAIATLLASGGLVVAAPAAYAASPTVVVAE
                     VYGGGGNSGATWRSDFVELFNRASTPVDLTGWKVSYWSAAGTTASSTLLSGTIAAGGR
                     YLVKEADGANTGATALPTPDAIGTLTMSASAGRVAVVDAAGAIVDLVGYGSTATSFEG
                     SGPAPGTTNSTSVSRIDACVDTDQNATDFAAGAPSPANSTTTPQTCTIAPPPPNPAET
                     IAAIQGASHRSPLAGTPVNGVTGIVTARSNTGFWMQDPVADADLATSEGIFVFTRTSP
                     TVAVGDALSVDGSVTDFRPGGSGGLDNLTTTEIVSPSVSITSSNNPLPAPVVIGVDRI
                     APAQTIDAGDPGSVENAAAVFDMARDAIDFYESMEGMRVAVRDARVVGPTASFGEIPV
                     VPGSVTGAPLTSSAGGVVYGSYDTPNAMRVQLDDALIPAGSMPAANVGDTLPGDAIGV
                     LDYSFANYKLLVTAAPTVTTGGLTRESTTAQTNNQLAVATFNVENLSPSNPQSKFDRL
                     AAQVVHNLASPDILALEEIQDNSGPANDGVVASDLTVSRLIAAIAAAGGPAYGSRWID
                     PQDGTDGGQAGGNIRQVFLYRLDRDLEFVDRPGGDATTAVEVTGTGRSTALSVSPGRI
                     APTSEAWSASRKPLVGEFRFRGETVFVVAVHFSSKGGDDPLFGRWQSPVRFTEEQRHL
                     QAAAVRDFADALLSADPQAKLVVAGDVNDFEFSTTTDLLVGQGSDALVDLPRTLPVAE
                     RWTYVFEGNSQVLDHILLSPALTVAPKGAKYPAYVYDIVHTNSPFYDQDSDHDPQIVR
                     LALRGGTGI"
     gene            17596..18690
                     /gene="ligD"
                     /locus_tag="PLB86_02080"
     CDS             17596..18690
                     /gene="ligD"
                     /locus_tag="PLB86_02080"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HRC63957.1"
                     /translation="MADAQVECPTPDGGTRAVRISSPDRVLWPQDGITKLDLAHYIRD
                     VGPAMLRRVGGRPMTLQRFPTGIEGEEFYSKNPPKGLPAHVRTVLMTYPSGRKHPQLV
                     VDEIATAVWGVQMNTVTFHPWPVRATDAEGALDRPDEVRIDLDPQPGCGFREAVEAAY
                     VLREVLAEAGLTGFPKTSGNRGVHVFCPIEPAREFLDVRHGVIAIARELERRMPDEVT
                     TAWWKEERGQRIFVDYNQACRDRTIAAAYSPRPLPGAPVSMPVSWESLRDIAPGDFTV
                     RTVPDLVGAQPDPWAAYDGAVGDLGVAMSWWERDVAEGLPEMAFPPDYPKMPGEPPRV
                     QPSKKRYEDEDYVPGGTGYLRAHPERPPRT"
     gene            complement(18724..19785)
                     /locus_tag="PLB86_02085"
     CDS             complement(18724..19785)
                     /locus_tag="PLB86_02085"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HRC63958.1"
                     /translation="MRQPIDLPVQPMLAKAVAEVPVGDNLAYEPKWDGFRCIVLRDGD
                     DIELASRGTKPLTRYFPEVVEHVRRLLPERCAVDTEIVVRSGGPGAERLDWERLSQRI
                     HPAESRVRKLSVETPAELVCFDLLAEGDESLLGMPFGQRRARLEAALGHLTDTDPIHL
                     TRMTTDPAIARDWFERFEGAGLDGVVAKPLDAAYAPGKRTMLKVKHKRTADAVVFGYR
                     VHTSGQGVGSLLLGLYGEWNGERLLIPVGGIAAFPMATRRALIDEFASLVVLDDEGQA
                     RHAATERSRFSSNKDSSFVPVRPERVVEVAFDQMEGHRFRHAVTFLRWRPDRDPQSCE
                     LDQVDRAPAYDLGEVLTVG"
     gene            complement(19845..20603)
                     /locus_tag="PLB86_02090"
     CDS             complement(19845..20603)
                     /locus_tag="PLB86_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRC63959.1"
                     /translation="MDLLIAPPEIQRHHPVGSRLDLRGLAAAYARPTGAQRWVSVNMV
                     STIDGAATGADGVTGSINSPADKIVFDLLRALSDIVVIGAGTARTEGYGPLTLDPEYA
                     ALRADLGLAPTLPLALVTRSGDIPDRLRQSATGSVMVITAASCPALAHLRADLGEEHV
                     VVAGEAAVDLVEAANTLARRGFQRIHSEGGPRLLGDLLRADVVDELCLTLAPHLVGGR
                     HKRIVEADDLRVSLVPHVLLHADGDLIGRWLVSR"
     gene            20689..21777
                     /locus_tag="PLB86_02095"
     CDS             20689..21777
                     /locus_tag="PLB86_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HRC63960.1"
                     /translation="MSKSKSKGAKKAAKKKESDKAAKAVKAVKVSKATKGSGKGASKS
                     KASKRGSSKAEPLASSPAALAAPNVYDVPVDVEPVEKKLRPDEPITSVLRVRRGFVLA
                     DFDPESTPGFDEGRKEGEEALSAFAPEMGAWQERLFAETKGGGKRSLLIVLQGLDSAG
                     KGGIVRHVMSNCDPAGIKATAFKAPTEEERQHDFLWRVRKALPGPGQIGVFDRSHYED
                     VLITKVRKLVPAGEITKRYAVINAFEREVIDSGTEILKVFMYISRDEQKARLMERLDR
                     PDKRFKYTPGDTDNRAYWDDYMKAFQVMLARTSTVAAPWYVIPANNKWYARYAVQQLI
                     LEKLRAMSPDWPVPDYDIEVEKARLADS"
     gene            21895..21967
                     /locus_tag="PLB86_02100"
     tRNA            21895..21967
                     /locus_tag="PLB86_02100"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21928..21930,aa:Gly,seq:gcc)
     gene            21988..22058
                     /locus_tag="PLB86_02105"
     tRNA            21988..22058
                     /locus_tag="PLB86_02105"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22020..22022,aa:Cys,seq:gca)
     gene            22089..22163
                     /locus_tag="PLB86_02110"
     tRNA            22089..22163
                     /locus_tag="PLB86_02110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22121..22123,aa:Val,seq:gac)
     gene            22375..25077
                     /gene="cas3"
                     /locus_tag="PLB86_02115"
     CDS             22375..25077
                     /gene="cas3"
                     /locus_tag="PLB86_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HRC63961.1"
                     /translation="MPFTDTDRIALLWAKSDAGGRPHSLVGHLLDTAAVAELIWDEFL
                     APGTRAHLDDAFAGHGRDLFRLVCGWHDLGKATPIFQAQRMDIAAAAIAAGIAVVPSS
                     GRWHHSQAGAMVVKQYLAARDLEGLEWIQHLIAGHHGIFAAGSRRAAPRGHGAGSGWG
                     PLQVHLADFVVASLRVDLPGLDRPAPELGLQLAVAGYVSMADWIASSSSFPGLGLTLV
                     SMSEARERAAAAWEQLHLHRGWDVNVLRSDNAMVRDRIGRSPRPLQELVADVSRGLAS
                     PGLVVIEAPMGEGKTEAAFIAAEILAARFGCNGFVFAMPTQGTTDVMWERCRRWAQGV
                     SPGMPVSLLHGKAMANEQWREMVETVSVTGVDLDDAYGPSAGFFAESPDFSEWLLGRH
                     RGLLAPAVVGTIDQMLYAAARVKYVALRHAGLVGKVVIIDEVHGYDVYMSQFLEALLV
                     WCANERIPVVLMSATLPSALRERLSQAYCAGVEERVAKGDRGTPSTSAHPKRGYPIVT
                     AISAADSPREVATVCWRPDLLVRVEVLADDSTVDVEPIARCVARATAGGGCALVVLNT
                     VARAQAVYRWLRDAGVPARLVHGRLTTAERAERAQALVESLGGGSRGSRPKRLVVVAT
                     QIAEQSFDVDADLLVTDLAPIDLLLQRIGRLHRHTQPQGTRPGALSEPRVIVTGFRTG
                     GPDGPILCPSFPYVYSEETLLRAAALVSDGAQWSIPSLVPDLVERAYAVVPEVPPAWR
                     ERVAEADRVGADLRAARAGRARTFVLRFQEATHTLSGLHEPVAQGDADEARVVRDGEP
                     THEVALIRRVDGEFVTLSGTALGPTGERCSITRIAREVLGDTVRVRETERVVGAEPLA
                     AWAHVPLLRRIDAVVLDEQGRGISDPRLRYDSEFGLVIERRGAR"
     gene            25131..>25317
                     /locus_tag="PLB86_02120"
     CDS             25131..>25317
                     /locus_tag="PLB86_02120"
                     /inference="COORDINATES: protein motif:HMM:NF021030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HRC63962.1"
                     /translation="MSTSYDLTVERWVPVIRGDGRPGLASLEEAMLDAHVIAGLDHSD
                     PLVAVALYRLLVALARDV"
     assembly_gap    25318..25417
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25418..26893
                     /gene="casA"
                     /locus_tag="PLB86_02125"
     CDS             <25418..26893
                     /gene="casA"
                     /locus_tag="PLB86_02125"
                     /inference="COORDINATES: protein motif:HMM:NF021030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HRC63963.1"
                     /translation="PLVAVALYRLLVALARDVADEGNAALVDQREWERALGAGRFDEE
                     VARRYFVKWRDRFDLFGEHPFYQVAGLEAVSGEVKSVSLLLPASATGNNVPLFAAMLD
                     SALPGLRPEEAARRVVACHAWDTAAIKTGAKGDPRVSAGKTTGNPTGPLGSLGAVIPL
                     GRTLFETILLSWMVGPQGTEDLPAWRRDHTAIWSERLPSGPKDLLTWQSRRIKLIPSW
                     DAAGQLHADAVIVAAGDRLPFSPPELEPHTLWRASGAAKAGVAVRPVRHQPGKSSWRG
                     LGGILAGRLPNPETGISTSGALVQLAATEALPYAYPLNLLLVGVVYGNQSAVIETVIV
                     DELPMPVQALHEDDQLRGDLETLATNAEACIYALNDLDANLRRSMGGDPTPWDKGQRP
                     GDRLAMALDDAARRVLRGIQREPERADEGLLAWEHFVLARAWEIANPLLDACSDTAFL
                     GRPDPRHRDRFLRQSTAEAFFRRSLRLALVRAHPQETKESQ"
     gene            26890..27510
                     /gene="casB"
                     /locus_tag="PLB86_02130"
     CDS             26890..27510
                     /gene="casB"
                     /locus_tag="PLB86_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HRC63964.1"
                     /translation="MSTATAGSLPARHYWDRLVDDQGRWRDGAPPGEALSALRRGFDS
                     EPGSVPGMWPFYTTLTGDGRVTASLRAEHLALAMFGLHQQSQSAPMHRAGDGLGKAMA
                     RLRASERYSADAIDRRFTQAATATDLDELGHHVRGLTTMLKTIAPPQPIDYTRLFQDL
                     CDWQRPDLVGRVRRRWGSDYFRLRSNPAEPGDPRPSTASSAGKDRS"
     gene            27507..28637
                     /gene="cas7e"
                     /locus_tag="PLB86_02135"
     CDS             27507..28637
                     /gene="cas7e"
                     /locus_tag="PLB86_02135"
                     /inference="COORDINATES: protein motif:HMM:NF020902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HRC63965.1"
                     /translation="MTSPTLYVDVHILQDLPPSNINRDDNGTPKQAVYGGVSRLRVSS
                     QAWKRAVRLEFAADLPKEATGVRTRRLTQLFTAELAERGLPESDATVLAAEAVKSLGI
                     TAGKKGDLSYLLFFSRGQLDTMASAVAAAAGDLTQSKTIDKAMLALGLKETLRAGHSL
                     DVALFGRMVADLADLNVDAASQVAHAISTHAAKTQFDYFTAVDDEQEEAETGAGMIGT
                     VEFNSATMYRFATVAVPQLAANLGDHAAAFDGVARFIRAFALSMPTGHQTSFAARTRP
                     ALVLVVVRQDQPVNLVSAFERPVWPGQQGVMAGSQEALADFYQTEVQRWGDQPALVVA
                     SYNAVDDKGHEKLVAAFGASRPLDEVIEAVGDVLRGLGRDHD"
     gene            28630..29424
                     /gene="cas5e"
                     /locus_tag="PLB86_02140"
     CDS             28630..29424
                     /gene="cas5e"
                     /locus_tag="PLB86_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HRC63966.1"
                     /translation="MTDRSLVLRLAGPMQSWGSSSEFNWRQTDAQPTKSGIVGLLAAA
                     SGRRREDPIEDLLALRLGVRIDQPGTLLRDYHTVSDFRGTPLPSAALNASGRQKPTSP
                     KKFTHVTQRYYLQDSVFVAVIGGNTQVLEGLASALRRPRFPLALGRRACVPTQPIVLS
                     EGAGADLWRGTVAEVIHRVPWQAGPAYRVSAADSSTVTVPFTVDDDNGDDDLADVPLS
                     FSREQRAKRSTRRVRHGWAELGSGSGATNAQAPQPARHNPFALLGW"
     gene            29427..30161
                     /gene="cas6e"
                     /locus_tag="PLB86_02145"
     CDS             29427..30161
                     /gene="cas6e"
                     /locus_tag="PLB86_02145"
                     /inference="COORDINATES: protein motif:HMM:NF020377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HRC63967.1"
                     /translation="MAYLSLITLNPLRRETQRLISSPQRLHAAVLRGLPQDDVTERVL
                     WRLERRERTMEVLVLTQRRPSWAALVESAGWPGADGGEGLVKDYDPLLARVAVGREFS
                     FRVVTNPTSTLSVLKSPTAQQRASLDDASRKRGIRVGHRTAGTQLGWFLARAAGDFDT
                     WGFTVGVPGDASVELVARRHESFIKGVDRRRVTLDTATFEGRLKVTDTERFTATLLGG
                     LGRGKAYGCGLLTLAPPREAAHVVEG"
     gene            30181..>30650
                     /locus_tag="PLB86_02150"
     CDS             30181..>30650
                     /locus_tag="PLB86_02150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HRC63968.1"
                     /translation="MSDRVSTVYVERCHVDRFENGIVIVNKERTVRVPAAYVAAVLLG
                     PGTRITHAAVALLADSGTVVEWVGEHGVRMYAAGLGPSRGSQVITRQAYLVTRTQERL
                     GVARAMYAMRFPGEDVAGLTMQQLRGREGARVKKIYKREAQRTGVAWATREYRAG"
BASE COUNT         4751 a         9911 c        10801 g         4987 t
ORIGIN      
        1 tggtggtggc cggcccggac gggcacggca tacctcatgt cttcgacggt gagccccccg
       61 gacgcccgag ccgcgcgcgc gagcggcgtg tcggccagag gatcgaccgc accggcttcg
      121 acagggtcgc cgggaggtgt tccgtgggcg ctgcggcggg ttcccgcggc atcggtgaga
      181 actgcccacc caccgacgag gccgacgagg gcggtcgtga gatcagtgac cccaccgccg
      241 gcgccgatga gttcggtgaa ccggtcgtgg gccgccgccg agcgctcgat gacctcggta
      301 tgccgccgga ccgcctcgtg ggcgacagac aacgcggcca aggctcgcgc cgtctcctga
      361 tgggcccgcg cctggaccat cgagacggcc gcgaggtcag cgagcgaccc gaggagggcg
      421 acctcctcct gcgtgaaggt atgccgcgtc cgatgcgcgg cgaagaggac cccgacgaac
      481 tcgccctcgg cgactagcgg ggtgccgcag atggcgacca aaccttcgtc ctggaccccc
      541 gtgtcgatcg tcgttgtgtg ggcgaagcgg tcgtcgtggg ggtagtcggt gctccaccac
      601 ggcgagaggg acgaggcgac caacccgccg agcccgaccc cgagctccag cctcagcgcg
      661 cggaaggtcg gggcgacagc gccgtcggtc gcccgcatgt aggtatcgcc gcgctcggga
      721 tcgtagaggg tgagatacga gacgtcggtg cccatgaggg cccgggcccg acgaacgatc
      781 gcatcgagca cccccgacgg gtcgcgcgcc gccgccagat cccgggcggt gtcgacgagg
      841 gctcgaagtt gaccctcgcg acggcgctgg tcctcggcgc gcagggcgac cgcgcgagcc
      901 aaccggtgcg cctcgggctg cgcgccgatc cggtcgaggt gatgggtcgg cgcgctgtcg
      961 gcgagcagcc gcagcagttc gagggacccg tcctgcgcca cgaccgtcga gcctaccgag
     1021 cggcgaccgc gcgcggcggc tgagagctca cgcgggtggc gcgacgaggg cgatctggtc
     1081 ggccagcgag cgcggctcga ccgcgccggc gaggtcgtcg atggcggcgg ctgcctcggt
     1141 cagctcagcg atcgtcggaa cgtggtcgaa gcgcacgccc ggtgcctcga gggtggcgac
     1201 ccgggcatag cgacccgcgc ccacggaata gacgttgccg gtggccgtgc agccgtcgct
     1261 cgcgagccag gtgacgagcg gtgacaccca ttcaggggcg agtcgctcga gcagttcggg
     1321 ggccatcatc gtttcggtca tcgccgactt cgccagtggc gcaatggcat tggcccgtat
     1381 gccggcccgc gcaccttcca gtgcgaggga ccgggtgaag ccgacgagac cggccttggc
     1441 ggcggcgtag ttgacctgac cgaagttgcc gtagagcccc gaacccgagg tcgtgttgat
     1501 gatcgagccg ccccgttcgg tgaggtgcgg ccaggcggcc ttggtcatgt ggaaggcccc
     1561 gtggaggtgg acggcgagga cggcctcgat catctcgggg gtggccttgg cgaagctctt
     1621 gtcgcggatg atccccgcgt tgttgaccag aacgtcgagg cgcccgaaag cctcgaccgc
     1681 ggccgcgacg gaaaccgccg caccctccca ggtgtccacc ccggcgacga ccccgagggc
     1741 cgaccctccg gcggccgtca cctcggcggc gaccgcagcc gggtcggtga catcgctgag
     1801 caccacgtga gcgccgagag cggcgaacgc gtgggcgtgg gcccggccga ggccgccggc
     1861 tgctccggtg atgaggacga cgcgtccggt catcgggtga gtggtcatgc ggagactcct
     1921 tcaagggtgg gggtggatgt ggtgcggggt cggaggaggg cggccagacc catgggctga
     1981 gggaggaaga tccgcgagtc catgagggcg acgtcctgca cgatcggtgc gaagtccatc
     2041 tgaccgagga cgtcgcgctc gatgtcgatg ccaggagcga cctcgatgag ccgcagtccc
     2101 tcgggggtgg cggcgaagac ggcgcgctca gtgacgtaga ggacccgctg acccagcgac
     2161 gcggcatacc gaccgttgaa ggtgacctgc tcgacgtgcc ggaccagttt gcggctgcgg
     2221 ccctcggaga ggattgtcag ccgtccgtcg gagatgccga cctgcatgcc gccggcggtg
     2281 aaggtgccga cgaagacgac ggtccgggcg ctctggctga tgtcgatgaa gccaccggct
     2341 ccggtgaggc gggggccgaa ccgcgagaca ttgacgttgc cgtcggcgtc gagctcggcc
     2401 atcccgagga ccgtgaggtc caagcccccg ccttggtaga agtcgaactg ctggttctgg
     2461 tcgatgaggg cgtccgggtt gagcgccgcg ccgaagtcgc cgcccgaggc aggcatcccg
     2521 ccgacgatcc ccggctcggt ggtgagggtg atccggtcga tgacgtgctc ctcggcggcc
     2581 accgtcgaca cgccgtccgg cataccgatc ccgaggttga cgacgccgcc atcgggcaac
     2641 tccatggccg cccggcgggc agcgatcttg cgctcgttca tcggcatcgg cgggatgagg
     2701 tctaacggga tgcgcacctc gtgggcatag ccggggttga aggcggtcgc gtaggtctgc
     2761 cggtgcagct cggggggagc gacgacgacg gcatcgacga ggatgtgggg gatcttgacc
     2821 aagcgcgggt cgagggtccc ggcggaaacg atccgctcga cctgaacgat gaccttgccg
     2881 ccgttgttgc gggcggcgca ggccatggcg aggttgtcga gggtgagcgc ctcgcgctcc
     2941 atggtgatgt tgccgtgctc gtcggcggag gtgccgcgca ggagggcgac gtcgatgggg
     3001 gtggcgcggt agaacagcag ctcctcaccg ccgagatgca cgacctcgac aaggtcgtca
     3061 gtcgtgcgct cgttgactcg gccaccctgg gagcgagggt cgacgaaggt atgcagaccg
     3121 atccgagaga ccgtgccggg caggccggcg gccatgtcgc ggtagagccg gctgatgacg
     3181 ccctggggga ggttccaccc ctcgatctga ccgctgacgg cgagagccgc cacacgggga
     3241 atcagccccc agtggccacc gacgacccgg cgcagcaggc ccggctcggc gagccggttg
     3301 aggccctgtt ctcctccgtc accttgtccg gccgcgaaga gcagggtgag cccggccggg
     3361 tcgccgctct cacggaagcg ccgagccagc ccgtcgagga gggcgtcggg ggtgccgcag
     3421 ccgacgaagc ctgacgtggc gatggtctcg ccgctgcgga tgagctccat ggcggactcg
     3481 accgaccgga cgagtgaggt gtgaggtccc gaggcgacgg atccgggagc agcggatccg
     3541 ggagcagggg aggcgatggg ggtcatgaag gcggacctcc gagtggacgg gtggggcgcg
     3601 gaaggcgcgg gcagcggtgc ggctcgcgct cagtccatca cccctgcgca ctcgtctggc
     3661 atgtgggcgg catacatgct cgacggccgg taatggtgga gccacacatc ggcggcgacg
     3721 ccgtgcctcg ccacgatgaa gacatcccct ctgaccccgc agcctccggg agagccatga
     3781 gcttcctcat catcattgcc gcgttggcct tcctcatgtg gatggcctat cgcgggcaca
     3841 gcgtcatcct cttcgcgccg atcgccgctc tcgccgcggt cctgctcacc gacccgtccc
     3901 tcgtcgcccc gatgttcacg ggcctgttca tggacaagat ggtcggcttc atcaagaact
     3961 acttcccggt gttcctgctc ggcgcgatct tcgggaaggt catcgagatg tccggcttcg
     4021 cccgggccat cgtcacctgg gtcatcaagg tcgttggcgc caaacgcgcg atcctctcga
     4081 tcgtcctcgt ctgcgcgctg ctcacctacg gtggcgtctc gctgttcgtg gtcgtgttcg
     4141 ccgtctaccc cttcgcggcc gagatgttcc gcatgggtga catccccaag cgcctcatcc
     4201 ccggcacgat cgctctcggc gcgttcacct tcacgatgga cgcactgccc ggcaccccac
     4261 agatccagaa catcatcccg acgaccttct tcaaaaccaa tgcgtatgcc gcccccatcc
     4321 tcggcttcgt cggtgcgatc ttcgtcttca gcctcgggat gctctacctc gagcgggccc
     4381 ggcgcaaggc gttggccaag ggcgagggct atggcgaggg gcacatcaac gagcccgagg
     4441 tcttcagcgg cgagcgcaag gtcaacccgg ccctcgccgt gctgccgctc gtcctcgtcg
     4501 ccgtcgtcaa caaggtcctt acctcgaaga tccccgagtg gttcggcgac aaggtgagct
     4561 tctcgccgac cgttgtcggc aagtccgacc ccgtcaccgt cgcagtctcc agcaacgtgg
     4621 cgatctgggc ggtcgagggc gcgctgctca tcggcatcct caccgtcatc gccctcgcct
     4681 ggaagccggt gtccagccac ttcgcccacg ggtccaagga ggccgtcgga ggggcgatgc
     4741 tcgccgcgac gaacaccgcg tcggaatacg gcttcggggc ggtcatcgct gccctgcccg
     4801 ggttcctcgt cgtcgccaac ggcctggcca agatccccaa cacgctcgtc aacgaggctg
     4861 tcacggtcaa cgtgctcgcc ggcgtcaccg gctccgcctc cggaggcctg tcgatcgccc
     4921 tcggcgctat ggccaacacc ttcgtcgaga acgcgacggc ggccggcata cccatggagg
     4981 tcctgcaccg ggtggcctcg atggccagcg gcggcatgga cacgctcccg cacaacggag
     5041 ccgtcatcac cctgcttgct gtgaccggcc tgacccacaa ggtgtcctac aaggacatct
     5101 tcgccatgac gatcatcaag accgtggcgg tcttcgtcgt catcggcttc tactacctca
     5161 cccacctggt ctgacggcga cgaagcgggg ccggaccacc cagtggtccg gccccgcttg
     5221 gtctgctcgc tcggtctggc tgcggtcaga tgtcgcggaa gacctcgatg gtcgccccga
     5281 ggttgttgag gcggtcggcc aggtcttcgt agccgcggtt gatgacgtag atgttgcgca
     5341 gcaccgaggt ccccggggcg gccagcatcg cgagcaggac gacgacgcct gggcggagtg
     5401 ccggtgggca catcacctcg gccgcgcgcc acttcgtcgg cccttcgatg agcacgcggt
     5461 gcgggtcgag caactgcacc ttggcgccga ccttggtgag ctcggtcaga tagatggcgc
     5521 ggttgtcata gacccagtcg tgcaacatcg tcgtgccctc ggccatcgcg gcgatgagcg
     5581 cgaagaacgg caagttgtcg atgttgaggc cggggaacgg cagcgggtgg atcttgtcga
     5641 tcggcgcctt cagcggcccg gggatcgttg tgatgtccac caaacgggtg tggccgtttg
     5701 ccgagaggta ctcgggggtc atcttgacct gcatgcccat gccttcgagc gtggccagct
     5761 cgatctccat gaactcgatc ggcacgcggc ggatcgtcaa ctccgactcg gtcaccactg
     5821 cggccgccaa cagggacatc gcctcgatgg ggtcctcgct gggggagtac tcgacgtcga
     5881 cgtcgatgtc ggccacaccg gtgaccttga gggtcgtcga tccgatcccc tcgatctgca
     5941 cgccgagctt gagcaggaag aagcacaggt cctgcaccat gtagttgggg ctggcattgc
     6001 ggatcgttgt cgtgcccggg ttgcgggcgg ccgccagcag gacgttctcg gtgaccgtgt
     6061 cgccacgctc ggtgaggatg atcggcttgt cgggctggtg aggggcggca tgggcctcgt
     6121 agaacccgtg ggtcgccacg acgttcaacc cgaacgaccg cagcgccgcc aggtgcggct
     6181 caacggtgcg ggtgccgagg tcgcagcccc cggcatacgg cagtcggaac tcggcgcggt
     6241 cgtgcagcag cggcccgagg aacatgatga ccgtgcgggt gcgtcgagcg gcgtcctcgt
     6301 cgatcccctc caggtccagc gtggcgggcg gcacgatctc caggtcgttg gagtccggca
     6361 gccaacgcac cttgaccccc atcgaggaca acacctcgat gatgcggttg acctcctcga
     6421 tccgggccag gttgcgcaac gtcgtgcggc cgcggttgag cagcgtcgcg cacagcaggg
     6481 cgactgcggc gttcttggag gtcttgacgt cgatggcgcc cgacaacttg gccccaccga
     6541 cgatccgcag atgctgcggg cgagaattcc cgagggtcac caactcgcgg tccaaggctt
     6601 ctccaatgcg ggcgagcatc tccagcgaca agttctggtg gccctgctcg atccgcgcca
     6661 ccgcgctctg actcgtcccc agcaagcggc ccaactcggt ctgagtgagc cccttgtgcc
     6721 gtctggcatc ccggatgagc gtgccaatcc ggctgcgata gtcctccgac atgccgccat
     6781 cgtatctcgc agatgagatg tcgcgagccg cgtggggcgc ctcaggcgca ggtatgccgc
     6841 ccgcttctgc gggcggcata cctcctgacg gatttgtccc taggcaggaa cgctcgcggc
     6901 tccggcgggg aggaaccggg cttcggcgac ctgctcggcg actccgctgc ggtcgagcaa
     6961 cggggtgagc ccgccgttcc agaactggaa gccggcaccg gtgatcatgg ccaggtcgat
     7021 gtccatcggg gcctgggcca ccccctcgtc gagcatcatc cgggcctcct gcgccaaggc
     7081 gccgagcacc cgttcgcgca cctcgggcgc cgtgaggacg accgggttct caggctgggg
     7141 gagcagagcg agcacctcgg ggtcgagggc cggcttgcca ccggcgaagg tgtagaagcc
     7201 aggcttcttg gcctcgacga tcgccttgag cccctcggag acgtagaacc ggtcggggaa
     7261 ggccttgatg agggtctcgc cgttgtgcag cgcgatcgcc ggaccgacca gcccgagcag
     7321 gacgaagggc ggcatcggcg cgaagcccat gaaggcctgg tcgatgacct cgacgggcgt
     7381 gccggcgtcg gcgacccgac ccacctcacc catgaaccgt ccgagcagcc ggttgacgat
     7441 gaaggacggc gagtccttga cgaggatgca cgtcttcttc agcttggctc cgacggcgaa
     7501 cgccgtggcc aaggtcgccg gatcggtggt cgagccgggg atgatctcca gcaacggcat
     7561 gacggcgacg gggttgaaga agtggaagcc caccactcgc tcggggtggg cgaggtcggc
     7621 cgccatctgc gagatcgaga ggctggaggt gttggtcgcc aggacgcagg tgtcggagac
     7681 gacggcctcg cactcggcga agacctgctt cttgacgccc atctcctcga agacggcctc
     7741 gatgacgaac tcggcatcgg cgaagccggc cttgctcgtc gacccgctga ccagcgcctt
     7801 gaaccggttg agcttgtcgg ggttcagtcg gcccttcttg gccagcccgt cgagctgggc
     7861 gtggacatag cccaccccct tgtcgacgcg ctcctggtcg aggtcggtga ggaccaccgg
     7921 cacctcgagg cgctggatga acagcaatgc cagttggctg gccatcaggc ccgcaccgac
     7981 gatgcccacc ttggtgaccg ggcgggcgag cgagcggtcc ggagccccgg ccgggcgctt
     8041 ggcgcgcttc tgcaccaggt cgaaggcata gaggccggcg cggagctcgt cgcccatgag
     8101 aaggtcggcc agcgcctcgt cctcggcggc gaaggcctcg tcgcgggtgg cggtgcgcgc
     8161 ggctgcgaca agcttcatcg cccggtacgg agccggtgac tgcttgcccg tcttgaggtc
     8221 ggccagcatc gtcgacgccg cgatggcgcc tgtccaggtg gcttcgtcgc gggggatctc
     8281 cggccgggtc acggtcacgg cgcccgagac gaccttcgcg gcccaatcga tggatgcctc
     8341 gaggaactcc gccggcgcga acatcgcatc ggccacaccc aactcgaacg ccgccgggcc
     8401 cttgagcatc cggttggtgt tgagcgcgtt ctcgacgatc atcgtcagcg ccttggccgg
     8461 gccgaccagg ttgggcagca ggtagcagcc gccccatccg gggaccaacc cgaggaagca
     8521 ctcgggcagc gccaccgcag ggacgcctgc ggagatggtg cggtagtcgc acgccagggc
     8581 gatctcgacc ccgccgccca tggccgcgcc gttgatgaac gcgaaggtcg ggaccgggag
     8641 gtccatgagg gtggcgtatg ccgcgtgccc ggctcgagcg atcgccagcg cctgctcgcg
     8701 ctgggtcacc ttggggactc cggagaggtc ggcgccgacg gcgaagatga acggcttacc
     8761 ggtgaggccg acagcctgga tctccccggc ctcggcgcga gcacgcaagc cgtcgaagca
     8821 cgcctgcaac ccggcgatgc tcgccgggcc gaaggtgttg ggcttggtgt ggtcgaagcc
     8881 gttgtcgagg gtgaccagcg ccatcgtgcc tgcctgaccc ggcatggtca cgtctcgaac
     8941 gagggccttg gtgacgacct cctccgggaa gagcgcggcg aagtcgacgg cagcggcgac
     9001 aggggcggca gtagtggtct cgctcatggc tcaggcctcc ttgctggccg acgcgacgta
     9061 gtcggcgtgg tgagggttct cccagatgac ggcgccgccc atgccgatgc cgatacacat
     9121 cgcggtcagg ccgtagcgca cctcggggtg ctccttgaac tggcgggcca gttgagtcat
     9181 caggcggacg cctgaagacg cgagggggtg accgatggcg atcgcaccgc cgtattcgtt
     9241 gacccggggg tcgtcgtcgg cgatgccgaa gtggtcgagg aaggcgacga cctgcacggc
     9301 gaaggcctcg ttgagctcga acagcccgat gtcctcgatc gtcaggcccg ccttggccag
     9361 ggccttctcg gtggcaggca ccggcccgat gcccatgacc tcgggctcga cgccgacgaa
     9421 ggcgaacgat gccaaccgca tgcggaccgg catacccatg gccacggcgg cgtcctcgga
     9481 ggcgagcaga cacgcggtgg cgccatcgtt gaggcccgcg ctgttgccgg cggtgacgtt
     9541 gccgcgcacc cggaagggcg tcttgagcgc ttcgaggtcg gccatggtgg tgctgggcct
     9601 cgggggctcg tcgacggtcg cgaggccgta gcccaactcc ttgtgccgag tggcgacgga
     9661 gacgaggtcg ggctggatct tgcctgcggc ataggcggcg gcgagcttgc cctgggagcc
     9721 gagggcgaag gcatcgcagc gggccttggt cagctgaggg aagcggtcgt ggaggttctc
     9781 ggcggtctgg cccatgacca gcgcggacgg gtcgacgatc ttctcggcga tgatccgcgg
     9841 gttggggtcc acgccctcgc ccatcgggtg gcgacccatg tgctcgactc cgccggcgat
     9901 ggcgacgtcg atggcgccca tggcgatcga cccggcgacc gcagtgaccg cggtcatggc
     9961 cccggcacac atccggtcga tggagtagcc gggagtcgtg ttgggcagac cggacagcag
    10021 ggcggcgacg cgaccgaggg tcaacccctg gtcaccgatc tgggtggtcg cggcgatggc
    10081 gacctcctcg accttgtcct tggggagctc cggatggcgc tcaagcagct cccggatgca
    10141 ccggatcacc aggtcgtcgg cgcgggtctg ggcgtagatg cccttgtcgc cggccttgcc
    10201 gtagggggtt cggaccccct cgacgaacac gacctcacgc agggtacggg tcatgctggg
    10261 tgcctccttg ggcaaacggt catggggcag tggtggctgc cctgagccga tgctaccggt
    10321 cagtaactcc tgcgcggaac ccagcagagc gttgccgcag tgagtcccgt cacgggtgct
    10381 cggcggccgc cgtcgccagg atgggcgcaa ccacgttgac ctgccattcg cgggccccac
    10441 ctccgcgcag cgcggccgcg aaggccgact cgccgaggtc gtcggggggt gtccacagaa
    10501 cccgacgcag cagatcgggg gtcagcaggt tctcgacggg gacgacccgc tcggaggcgt
    10561 atgccgccat ggcatcccgg gcgcgcccga gccgggccgc ggcgatgggg tcgcggtcgg
    10621 cccagaggcg aggaggcggg gggccggtgc tcgacagtgt caacggaggc aaggcatcct
    10681 ccgagagggc cagcgccttg tcgatggcgt ccagccacac cttggggctg cgtgcgactc
    10741 cacgcaactc ccgtcgagca gcgaaggcgc cggcgtcctt ggggggctcg acggccaagg
    10801 cgaccagtgc agcgtcggac agcacccggc cgggggacac gtcgcgacgt tgggcgatcc
    10861 ggtcgcggac gtaccagagc tcgcggacga cggcgactcc gcggcgacgt ctgatcttgt
    10921 gcatgccgga ggttcggcgc cacgggtcga cccgcggcgc gggaccagtg aaaccgagca
    10981 gcgagtcgaa ctcctgggcc gcccactccc acttgccctg ctcggtcagg tcggcggcga
    11041 tcgcgtcgcg taggtcgacg agcacctcga cgtcgagggc ggcataccgc agccatggct
    11101 cgggcagcgg gcggcgcgac cagtccaccg cggagtgttc cttggccagg ctcagcccga
    11161 ggtagtgctc aacggcggca cccaacccga cccgggggag ccctgccagg cgcccgccca
    11221 gctcggtgtc gaacaaccgc tgcgggcgca ggcccacctc ggccagacag gccaggtctt
    11281 gggtcgctgc gtgcagcacc cattcggcgt cgccgatggc ctcggccacg ggggagaggt
    11341 cggggcaggc tgcggggtcg aacagccagg tccccgaccc ggcacgacgc agttgcacga
    11401 gataggcacg ttgtccgtag cggtggcccg aggcccgctc ggcgtcgagg gcgaccgggc
    11461 cagtccctcg cgcgatcgcg gccgcagcgg ccatgagttg ccgttcgtcg atgacgacct
    11521 cggggacgcc gtcggcggga aacagcaacg gtgtcgcttc gggtcccgcg ggcggctcag
    11581 cggctgagtc agacgcagtc cccacagaag gatcaaccga ggactcggcc gagannnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcggct gagtcagacg cagtccccac
    11761 agaaggatca accgaggact cggccgagag cgcgtcgtcg gtctggtcgt ggtccggctc
    11821 gagctcggtc accgacgggc cccgggcaag gcgaccacgc cgtcgggcag tggcggcagc
    11881 ccggcgatcg tgctcaacag atcagaccag gccagcaggt ggtcgccgag gtcgccgccc
    11941 aacggagtcc acgaagcccg aacctcgatc tccacggttg gcaactggtc ggccaggccg
    12001 ccgaaccgct ccgagacgac tcgggtgacc gtgccggcct cggcgcagta gcgcaggccg
    12061 cgcgcctgta gggcttcgat gagccaggcc caacccacct caccgagcga ggggtcggtg
    12121 cccacctcgg gctccaactc ggcgcgggcg aatgtgacgg ctcgccaggc gccttcccat
    12181 ggctcggggc aactggggtc gtgcagcagg acgaagcgac cagatgccag ctcctcctcg
    12241 cctccggggc cgacgacctc gccggacagc gccacggcat acggagcgat gcgttggggc
    12301 gcaggcacct cggtgaggcg cacttccggg cgcagccgag cctcccgcag gccggcaatg
    12361 gcccgggtga attcagcggc ggtgtcgccg gggagggatc gtcctcgcac gtcactgaga
    12421 gtaggcgggc cgaggtcccg atgggcgtcg gacgcgccga cagtacgctc gcgaaccggg
    12481 taccgtgccg gtgtgccagc cctcctcgcc ctcctgtcag cagccgtatg gggcgcgagc
    12541 gacttcgccg ccggggtcat ctcccggacc cttcgtccag tggttgtcgt cgcgtggtcg
    12601 cagtcgatgg ctctcgtcct gctgacggcg atcgtcgcgt tccgcgggtt gcccgagcag
    12661 gggttcgccg ggtggttccc ctgggcgatg ctcgccgggg tcagcggcgc gatgggcctg
    12721 ttgtgtttct atgccgcgct ctccacgggg accatgggtg tcgtcgctcc cattgccgcc
    12781 gtcggctcgg gcatccccgt gttgctcggg gtgctcagcg gcgagaagcc ctccgccttg
    12841 gcctggtggg ggatcgccgt cgccatcgcc ggggtggtgc tggcgagcgg gccggagatg
    12901 aaggagggcc tgggcgcccg accggtgctg ttggcgtgcc tggccgcggt cggcttcggc
    12961 gtcgctctgt tcgccctcga ccgcggcgcg cgcgtgtcgc tgctgcacac cttgtggggg
    13021 atgcggatca ccagcgccat cgggtatgcc gcgctggcgc tcgccgcgcg ctccctcggt
    13081 ggggtcgccc gccgccacgt gccggtgctg gcggccgtcg gcgcaggtga tcttgccgcc
    13141 aacgcgctct tcgggatggc ctcgtcgatg ggcatggtca gcgtcgccgc cgtcctcgga
    13201 tcgctctacc ccgtgatgac gatcctgctg gcccgctact tcctgaacga acggctgcgc
    13261 aggatccagg tgatcggctc gaccttggcc ctctgcgggg tcgttctgat cgccgcaggc
    13321 gggcggggct gaccccgccc gcctaggcac gtcggctgat tcaggccttc tcgctcgtgc
    13381 cctcggaggc cgccggtccg gcggtggctg ctgccggctc cggctcgcgg acgggcgctg
    13441 cgggtgtcga ggcagcccat gacggtgggg tggtgggtgt cgcctcctga cgctcggagc
    13501 gccgacgcag ccaggaccgg accgggatcg caatcagggc caggacgatg agccagggca
    13561 gagccgcccc caggacggtc aggcccaccg tgaggctggc caggaaggcg ttccagcctg
    13621 accgcagacc tgccaggaag ccgtcctgct cgaccggtgg gatcacttcg gggttggagc
    13681 tcaccgtcac ggtgacggtc gccatcgcga ttcgtttcgt catcgacgcc agctgcgcct
    13741 gcaacgactc aaggtcggct tcgcgctggg ccagttggct ctccaactcg acgatctggc
    13801 tgatgttctg ggtctggctc atcagcgctc gcacccgctc gatgctcgcc ttcttcgtcg
    13861 cgatgcgcga ctcggtgtcg acgtatgccg cggtgacgtc atcgctcgcg ctggctcgct
    13921 gcacgacggt gccgagtttc gagagttgat ccaaggcggc gtcgagccgg tcggtcggga
    13981 tcgacagcac caactggccg tagttgccga cccgccgcac cggcgcggtt gccttcccac
    14041 cgatcgcggg atcggcgctc ggtgccactt cggttgcgtt gaccgtctcg ctggcgaccg
    14101 ctccgcccaa gccggccgca acgccacgca cggcggctgc ggcagtctcg acggaggtga
    14161 cggtgagata gatgctcgcg gtgcggatga tcttggtgtc cacgagtgct gcttcgccca
    14221 ctgacgcgac gtcaggagcc gacagcttgg tgtcgcccgc tgcagcggca ccaacagcgg
    14281 cgccgggcgc cggagccggc gcaacggcca ctccgccctc gcgggcggcg ttcgtgctgg
    14341 tcggtgacga cccgctggag caggctccca gggcgaggag cgcggcaaca gtgagtgcgg
    14401 ctggtcgcaa ccggcgggac acggcatacg aaaacatcgg ggtcctcctc gggcgggccg
    14461 gacggggccg gcgatggagc gttcggccct tggaggggga gccgcccggg tgtggttccc
    14521 aagcgcgcgt ctcgatcagg tctcgatcag gtgcgaggtt ccaaggtcag gcagaccgag
    14581 ttgatgcagt agcgctgatc ggtgggcgtg ccatagccct cgccctcaaa gacgtgcccc
    14641 aggtgggagc cgcagccggc gcaccggacc tcgacgcggg gccgaccggg gatcgagcgg
    14701 tcctcgatgt attcgacccg gtccccggcc agcggctcga agaacgacgg ccacccgcag
    14761 tgcgaatcga acttcgactc gctgcggaac agttccgcgc cgcaggcccg gcaggagtag
    14821 accccgtctg tcttcgtgtc ggtgtactcg ccggtgaacg ggcgttccgt gcccgcctcg
    14881 cgcaacacat ggaactcggc cgccgagagc ttggcccgcc actgggtgtc ggtgagttgc
    14941 acggcatacc gccccggggt ggggacggcg tcggcgggcg ggacggactg cgtggaggtg
    15001 gggtcggtca tggtggggat aacgcacgaa ggccccggga tcgtccccgg ggccttcgtg
    15061 cgctcggatc cgagccggtc agatgccggt gccgcctcgg agggccagcc gcacgatctg
    15121 cgggtcgtgg tcggaatcct ggtcgtagaa gggggagttg gtgtggacga tgtcgtagac
    15181 gtaggccggg tacttggctc ccttgggcgc cacagtcagg gcggggctga gcaggatgtg
    15241 gtcgaggacc tgcgagttgc cttcgaacac gtaggtccag cgctcggcca caggcagcgt
    15301 ccggggaagg tcgacgagag cgtcggaacc ctggccgacc agcagatccg tcgttgtgct
    15361 gaactcgaag tcgttgacgt caccggcaac gacgagcttg gcctgggggt ccgcgctcaa
    15421 cagtgcgtcg gcgaagtccc ggaccgcggc cgcctgcagg tggcgttgct cctcggtgaa
    15481 gcggaccggt gactgccaac ggccgaagag cgggtcgtca ccgcccttgg aggagaagtg
    15541 gacggcgacg acgaagacgg tctcgccgcg gaagcggaac tctccgacga gcggcttgcg
    15601 gctggccgac caggcctcgc tcgtgggggc gatccggccc ggcgagaccg acaatgccgt
    15661 gctccgaccg gtcccggtca cctcgaccgc agtcgtcgcg tcgccacccg ggcggtcgac
    15721 gaactccagg tcgcgatcga gccggtagag gaagacctgg cggatgttgc ctcccgcctg
    15781 cccgccgtcg gtcccgtctt gcgggtcgat ccagcgggat ccgtatgccg gtccgccggc
    15841 ggccgcgatc gccgcgatca ggcggctgac ggtgaggtca gacgcgacga caccgtcgtt
    15901 ggccggcccg ctgttgtcct gaatctcctc gagggcgagg atgtcggggc tggcgaggtt
    15961 gtggaccact tgggcggcga gtcggtcaaa cttggactgc ggattgctgg gcgacaggtt
    16021 ctcgacgttg aaggtcgcca cggcgagttg gttgttcgtc tgggcggtcg tcgactctcg
    16081 ggtgagaccg ccggtcgtca cggttggcgc agcggtgacg agcagtttgt agttggcgaa
    16141 cgagtagtcc aagacgccga tagcgtctcc aggcagggtg tcgccgacgt tggcggcagg
    16201 catggacccc gccgggatga gggcgtcgtc cagctggacg cgcatggcgt tgggggtgtc
    16261 gtaggacccg tagacgacgc cgccggccga actcgtgagc ggcgcccctg tgaccgaccc
    16321 cgggacgacc gggatctcgc cgaaggatgc cgtggggccg acgactctgg cgtccctgac
    16381 cgcgacgcgc atcccttcca tggattcgta gaagtcgatg gcatcccggg ccatgtcgaa
    16441 gacggctgcg gcgttctcca cgctccctgg gtcacctgca tcgatcgtct gggcgggggc
    16501 gatgcggtcc acgccgatga cgacaggggc aggcaggggg ttgttcgagg acgtgatgct
    16561 cacgctgggg gagacgatct cggtggtggt gaggttgtcc aggccgccgc tgccgccggg
    16621 acggaagtcg gtcaccgagc cgtcgacgct caaggcgtca ccgaccgcga ccgtggggga
    16681 ggtgcgggtg aagacgaaga tcccctcgct ggtggccagg tcggcgtcag cgaccggatc
    16741 ctgcatccag aagccggtgt tggagcgggc cgtgacgatg cccgtcactc cgttgaccgg
    16801 agtgccggcc aggggtgagc ggtgggacgc accctggatc gcagcgatgg tctccgccgg
    16861 gttcggcggg ggcggggcga tggtgcaggt ctggggtgtg gtggtggagt tggcggggga
    16921 gggcgctccc gctgcaaagt ccgtcgcgtt ctggtcggtg tcgacacagg cgtcgatccg
    16981 gctgaccgac gtcgagttcg tggtgccggg agcgggcccg ctgccctcga aggacgttgc
    17041 ggtgctgccg tatccgacga ggtcaacgat ggcacccgct gcgtccacga cggctacccg
    17101 gccggccgag gccgacatcg tgagcgtgcc gatggcgtcg ggagtcggca gcgcggtggc
    17161 tcccgtgttg gcgccgtcgg cctccttcac caggtagcgc cccccggcgg cgatggtgcc
    17221 ggacaggagg gtgctgcttg cggtcgtgcc tgcggctgac cagtaggaga ccttccaccc
    17281 ggtgaggtcg acgggggtgg aggcgcggtt gaacagttcc acgaagtcgc ttcgccacgt
    17341 cgcgccggag ttgccgccgc cgccgtagac ctccgcaacg accacggtgg gagaggctgc
    17401 gtatgccgcg ggggcagcga ccacgaggcc gccggaagcg aggagggttg cgatcgcgag
    17461 ggtgacagcc ggtctgcgga catgggtcca gacgtgggac ataggtgtgt cctgtcgctc
    17521 gggagagtgg tgcctcgaac gtacggtgtc gcggcaggtc acgcacccgg aacggtgcgt
    17581 gcaatagtga ccgacatggc tgatgcccag gtggaatgcc cgacccctga cgggggaacg
    17641 cgggcggtgc ggatctccag cccggaccgg gtgctgtggc cgcaggatgg catcaccaaa
    17701 ctcgatctgg cgcactacat ccgggacgtc gggccggcga tgctgcgccg ggtcggcggt
    17761 cgcccgatga cgttgcagcg cttcccgacc ggcatcgagg gggaggagtt ctactccaag
    17821 aacccgccaa aaggcctgcc ggcccacgtc cgcaccgtgc tgatgaccta cccctccggg
    17881 cgcaaacatc cccagctcgt cgttgacgag atcgccacgg cggtctgggg cgtgcagatg
    17941 aacaccgtga ccttccaccc gtggccggtg cgcgccaccg atgccgaggg tgcgctggac
    18001 cgacccgacg aggtccgcat cgacctggac ccgcagccgg ggtgcggctt ccgggaggcg
    18061 gtggaggcgg catacgtgct gcgtgaggtg ctcgcggagg cggggctgac gggcttcccg
    18121 aaaacgtcgg gcaaccgggg cgtgcacgtc ttctgcccga tcgagccagc gcgggagttc
    18181 ctcgatgtgc gccacggggt gatcgcgatc gcccgcgagt tggagcgccg gatgcccgac
    18241 gaggtgacca ccgcgtggtg gaaggaagag cggggccaac ggatcttcgt cgactacaac
    18301 caggcctgcc gggaccggac catcgctgct gcctacagcc cgcggcccct ccccggtgcg
    18361 ccggtgtcga tgccggtgtc gtgggagtcg ttgcgggaca tcgctcctgg tgacttcacg
    18421 gtccgcaccg ttccggatct tgttggcgcg cagccggatc cgtgggcggc atacgacggc
    18481 gcggtggggg acctcggggt ggcgatgtcg tggtgggaac gtgacgtcgc tgaggggttg
    18541 cccgagatgg ccttcccgcc ggactacccg aagatgccgg gggagccgcc gcgggtgcag
    18601 ccgagcaaga agcgttacga ggacgaggac tacgtgccgg gcggcacggg ctacctgcgc
    18661 gcgcatcccg agcgaccgcc gcgcacctaa cggcgttggc tccgcgtggg cagacctcgg
    18721 ccgtcagccg acggtgagca cctcgccgag gtcgtaggcc ggggcccggt cgacctggtc
    18781 cagctcgcag gactgcgggt cgcggtcggg tcgccagcgc aggaacgtca ccgcgtgccg
    18841 gaaccggtgt ccctccatct ggtcgaaggc cacctcgacg acccgttcgg ggcgcaccgg
    18901 cacgaaggag ctgtccttgt tggacgagaa gcgtgaccgc tcggtcgccg cgtgccgggc
    18961 ctggccctcg tcgtcgagga ccaccagcga ggcgaactcg tcgatcagcg cgcgccgggt
    19021 ggccatcggg aaggccgcga tcccgccgac ggggatgagc aggcgctcgc cgttccactc
    19081 gccatagagc cccagcagca gcgaccccac gccctgccca gaggtgtgca cgcggtagcc
    19141 gaagaccacc gcgtccgccg tgcgcttgtg cttcaccttg agcatggtgc gcttccccgg
    19201 ggcgtatgcc gcgtcgagcg gcttcgcgac gactccgtcc aggccggcac cctcgaaccg
    19261 ttcgaaccag tcgcgggcga tcgcggggtc ggttgtcatc cgcgtgaggt ggatcgggtc
    19321 ggtgtcggtg aggtggccca acgcagcctc gaggcgcgcg cggcgctggc cgaacggcat
    19381 accgagcagg ctctcgtcgc cctcggcgag gaggtcgaaa cagaccaact cagcgggggt
    19441 ttcgacggac agtttgcgaa cccgtgattc ggccggatgg atgcgttggg acagccgttc
    19501 ccaatcgagg cgttcggcgc cggggcctcc ggaacgcacg acgatctcgg tgtcgaccgc
    19561 gcagcgctcc ggcagcaacc ggcggacgtg ctcgacgacc tcggggaagt agcgggtgag
    19621 tggtttggtg ccgcggctgg ccaactcgat gtcgtcgccg tcgcgcagaa cgatgcagcg
    19681 gaacccgtcc cacttgggct cgtaggccag gttgtcgccg accggcacct cggcgaccgc
    19741 cttggcaagc atcggctgga cggggagatc gatgggctgg cgcatcgggc cattgtgcgc
    19801 catgacgtga gggtcggtcg tgagcgacgg ccaggcttgc cgggtcagcg gctgacgagc
    19861 caccgcccga tgaggtcgcc gtcggcgtgc agcaggacat gcgggacgag cgagacgcgc
    19921 agatcgtcgg cctcgacgat gcgcttgtgt cggccaccga cgaggtgcgg tgccaacgtc
    19981 aggcacagtt cgtcgacgac gtcggcccgc agcaagtcgc cgagcaggcg tggcccgccc
    20041 tctgagtgga tgcgttggaa acccctgcgc gccaacgtgt ttgcggcttc cacaaggtcc
    20101 acggcggcct cgcccgccac gacgacgtgt tcctcaccga ggtcggcgcg caggtgcgcg
    20161 agggcagggc acgaggcggc ggtgatgacc atgacgctgc cggttgcgct ctgtcgcaac
    20221 cgatcaggga tgtctcccga ccgggtcacc agggcgagcg gcagcgtcgg cgcgaggccg
    20281 agatcggcgc gcagcgcggc atactccggg tcgagggtga gcgggccata accctccgtg
    20341 cgcgccgtcc ccgcgccgat gacgacgatg tccgagagcg cgcgcagcag gtcgaaaacg
    20401 atcttgtcag cggggctgtt gatcgatccc gtgacgccgt cggcgccggt cgccgcgccg
    20461 tcgatggtgc tcaccatgtt gacgctcacc cagcgttgtg cgccggtcgg tcgggcgtat
    20521 gccgcggcga gacctcgcag atccagccgg ctcccgaccg ggtgatgacg ctggatttcc
    20581 gggggtgcga tgagcaggtc catcaccgaa gtatgcgggc cgagcccgtg tcggcgtgtt
    20641 cgcgaaggtg ccctcgaact gacggggacg ttacggcaag atgtctgcat gtcaaagagc
    20701 aagagcaagg gcgccaagaa ggcagccaag aagaaggaat ccgacaaggc cgctaaggcg
    20761 gtcaaggccg tcaaggtctc caaggccacc aagggctcgg gtaaaggcgc cagcaagagc
    20821 aaggcgtcca agcggggctc ttcgaaggcc gaaccacttg cctcctctcc cgctgcgctg
    20881 gccgcgccga acgtctacga cgtgccggtc gacgtcgagc cggtcgagaa gaagctgcgc
    20941 cccgacgagc cgatcacctc ggtgcttcgc gtgcgtcgcg gcttcgtgct ggccgacttc
    21001 gaccccgagt cgacgcccgg tttcgatgaa ggccgcaagg agggtgagga ggcactgtcc
    21061 gccttcgccc cggagatggg cgcgtggcag gagcggctct tcgccgagac caagggcggc
    21121 ggcaagcgca gcctgctcat cgtcctgcag ggcctggaca gcgcgggcaa gggcggcatc
    21181 gtccggcacg tgatgagcaa ctgcgacccg gccggcatca aggccaccgc gttcaaggcg
    21241 cccaccgagg aggagcgcca gcacgacttc ctgtggcgcg tccgtaaggc cctgccgggg
    21301 cctgggcaga tcggcgtctt cgaccgttcg cactacgagg acgtgctgat caccaaggtg
    21361 cgcaagctcg tccccgcggg cgagatcacc aagcgctacg cggtcatcaa cgccttcgag
    21421 cgcgaggtga tcgactcggg caccgagatc ctcaaggtgt tcatgtacat ctcgcgcgac
    21481 gagcagaagg cccgtctcat ggagcggctg gaccgccccg acaagaggtt caagtacacg
    21541 ccgggcgaca ccgacaaccg tgcctactgg gacgactaca tgaaggcgtt ccaggtgatg
    21601 ctcgcgcgca cctcgaccgt ggccgccccg tggtacgtca tcccggcgaa caacaagtgg
    21661 tacgcgcggt atgccgtgca gcaactgatc ctcgagaagt tgcgggcgat gagcccggac
    21721 tggccggtgc cggactacga catcgaggtc gagaaggccc gtctcgcgga cagctgagcg
    21781 ggtcggtcag ggcgaggcct ggcgggctgg atcggggcgg tttggacacg ccccggtccg
    21841 tccgctagtg tctactttcg caccagacgg ggaagttaca ccggtggtgt tcctgcggac
    21901 gtagctcagt tggtagagcg caaccttgcc aaggttgagg tcgcgagttc gagcctcgtc
    21961 gtccgctcgg aggtagaccg ccttcacggt ggagtggccg agaggcgagg cagcggcctg
    22021 caaagccgcg tacacgggtt caaatcccgt ctccacctcg ggctccacca aggagtcagc
    22081 agtaccgagg gcgattagcg cagtggtagc gcgcttcctt gacacggaag aggtcactgg
    22141 ttcaaaccca gtatcgccca ccacctcgaa acggcccctg acctgcgcaa atgcagggca
    22201 ggggccgtta gcgttcagtc cgcgttcgtc agataggcga cacgcgtgtg catctctggg
    22261 acatgctgcc cagggctgaa tggcgggatc tgttcctgcc ctcagcccca attcatagga
    22321 actttgcctc ccctggacga cccgttgtcg gtggtgtgag acatgatctg atccatgccg
    22381 ttcaccgaca ccgaccgcat cgccctgctg tgggcgaagt ccgacgccgg gggacgccca
    22441 cattccctgg tgggacacct gttggatacg gccgcggtcg ctgagttgat ctgggatgag
    22501 tttttagctc caggtacccg cgcgcatttg gacgacgctt tcgccggcca cggacgggac
    22561 ctgtttcgcc tcgtctgcgg ctggcatgac ttgggtaagg caacacccat ctttcaggca
    22621 caacgaatgg atatcgctgc ggctgccatc gccgccggta tcgctgtcgt gccgagttcc
    22681 gggcgctggc accactctca ggccggcgcg atggtggtca agcagtacct tgccgctcgc
    22741 gatcttgagg gccttgagtg gatccagcac ctcatcgctg ggcaccacgg catcttcgct
    22801 gctggatcgc ggcgtgctgc gccacgaggg cacggtgcag ggtcgggttg gggtccgttg
    22861 caggtgcatc tggccgactt cgttgtggcg tcgttgcgag tcgacctccc gggtctggat
    22921 cgaccagccc ccgagctggg ccttcaactc gccgtagcgg gctacgtgtc gatggccgac
    22981 tggatcgcaa gttcatcgtc gttccccggg ctgggcctca ccctcgtgtc gatgtctgaa
    23041 gcgcgtgagc gcgccgcggc ggcatgggaa caactgcatc tgcacagagg ctgggacgtc
    23101 aatgttctgc gcagcgacaa cgcaatggtc cgggatcgga tcggacgctc accgcgaccg
    23161 ctgcaggagc ttgtggcaga tgtttcacga ggtctggcat ccccgggcct ggttgtcatt
    23221 gaggctccca tgggcgaagg caagaccgag gccgccttca ttgctgccga gatccttgct
    23281 gcgcgattcg gctgtaatgg ctttgtcttc gcgatgccca ctcaaggtac gactgacgtc
    23341 atgtgggagc gttgtcgcag atgggctcaa ggagtgtcgc ccgggatgcc cgtgtcgctt
    23401 ctgcacggca aggccatggc taacgagcag tggcgtgaga tggtggaaac ggtttccgtc
    23461 accggggtgg acctcgacga tgcttacggc cccagcgccg gcttcttcgc tgaatcccct
    23521 gatttctcag agtggctact cggacgccat cgaggtctgc tcgcccctgc agtcgtcggc
    23581 acgatcgacc agatgctgta tgccgctgcg cgcgtcaagt acgtcgcgtt gcggcatgcc
    23641 ggactcgtcg gcaaggtcgt gataatcgac gaagtccacg gctacgacgt ctacatgagc
    23701 cagttccttg aagctctgct cgtgtggtgt gcaaacgaac gcatcccagt ggtgctcatg
    23761 tcggccaccc tccccagcgc cctgcgcgag aggctcagtc aggcctactg cgcgggcgtc
    23821 gaggagcgag ttgcaaaggg ggaccgggga acccccagca cgtctgccca tcccaaacgg
    23881 ggatacccca tcgtgacagc catcagcgcg gcagactctc ctcgggaagt tgccaccgtc
    23941 tgctggcggc cagacctcct cgttcgggtt gaggtgctgg cggacgactc gaccgtggac
    24001 gtggaaccga tcgccaggtg tgtagcccga gcgaccgcag gtggtggttg cgccctcgtg
    24061 gtcctgaata ccgtcgctcg agctcaggcg gtctatcgct ggctgcgaga tgctggggtt
    24121 ccggcaaggc ttgtccacgg acgactgacc actgcggaac gcgccgagcg tgctcaagcg
    24181 cttgtcgaaa gcttgggcgg tggcagccgt ggctcacgtc ccaaacggct cgtcgttgtc
    24241 gccacccaga ttgctgagca gagcttcgac gtcgacgctg acttgcttgt caccgatttg
    24301 gctcccatag acctgctcct gcaacggatc ggccgcttgc atcggcatac ccaaccgcag
    24361 gggacccgtc ctggtgcatt gagcgagccg cgggtcatcg ttacgggctt ccgtaccggt
    24421 gggcctgacg ggccgatcct ctgcccaagc ttcccgtatg tgtattccga ggagacgttg
    24481 ttgcgagcag cggccctcgt gagcgacggc gcgcaatgga gcattccctc gctcgtcccc
    24541 gacctcgtcg agcgcgcgta tgccgtggtc cccgaggtac cgccggcgtg gcgggaacgg
    24601 gtggccgagg cggaccgggt aggggcagac ctgcgtgctg cccgtgccgg gcgagcgcga
    24661 acgttcgtcc tacgattcca agaagcgaca cacacgctct ctggcctgca cgagccggtc
    24721 gctcagggcg atgccgacga ggctcgggtg gttcgggacg gtgagccaac gcatgaggtg
    24781 gcgttgatcc ggcgcgtcga cggcgagttt gtcaccttgt cgggtacggc cttggggccg
    24841 acaggtgagc ggtgttcgat cacccgaatc gcccgcgagg tgttgggtga cacggtccgg
    24901 gtccgcgaga ccgaacgtgt ggtcggcgcc gaaccccttg ccgcgtgggc gcatgtcccg
    24961 ttgcttcgcc ggatcgatgc agtcgtcctt gacgagcagg gccgcggtat atccgacccg
    25021 cgccttcgct acgacagcga gttcggtttg gtcatcgagc gccgaggcgc ccgatgacca
    25081 ggggacgtct tgggctccca aatgagtgcg tcaaagaagg aggagcgact ttgtcgacaa
    25141 gctacgacct cacggttgaa cggtgggttc cagtcatccg gggtgacgga cgtccagggt
    25201 tggcctcgct tgaggaggcg atgcttgatg cccacgtgat cgctggactg gaccactcag
    25261 atccccttgt tgcggtagcc ctataccgcc ttctcgtggc cctcgctcga gacgttgnnn
    25321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc cttgttgcgg tagccctata
    25441 ccgccttctc gtggccctcg ctcgagacgt tgccgacgag ggcaatgctg cacttgtcga
    25501 ccagcgtgag tgggagcgtg cccttggcgc cggtcgattc gacgaggagg tggcccgtcg
    25561 ctacttcgtg aagtggcgag accggttcga tctgttcggg gagcacccgt tctatcaggt
    25621 tgccgggctg gaagcggtga gcggcgaggt caagtcggtg agtctccttc tgccagcgtc
    25681 cgccactggc aacaacgtcc ctttgttcgc agccatgctg gacagcgctt tgcctggatt
    25741 acgtcccgag gaggctgcgc gccgcgtggt ggcttgtcat gcatgggata ccgccgcgat
    25801 caagacgggc gccaaggggg acccaagggt ctcggcgggc aagaccacgg gcaatccgac
    25861 tggtcccttg ggcagcctcg gcgccgtcat cccgttggga cgtacgctct tcgagaccat
    25921 cctgctgagt tggatggtcg gcccccaagg cactgaggac ctgccggcgt ggcgtcgcga
    25981 ccacacagcc atttggtctg agcgcctgcc aagtggaccg aaggaccttc ttacctggca
    26041 gtcgcgacgg atcaagctca tcccttcctg ggatgcggcg ggacagctcc atgccgacgc
    26101 ggtcatcgtc gcagcgggtg atcggctgcc gttcagccct cctgagcttg agcctcacac
    26161 gctatggcgg gcgtctgggg cggccaaggc gggcgtagcg gtgcgacccg ttcgccacca
    26221 gccgggcaaa tcctcatggc gcgggctcgg cggcatactt gcgggccgcc tgccaaatcc
    26281 ggaaacgggg atctcgacgt ccggagcgct cgtgcagctg gccgccaccg aggccttgcc
    26341 atacgcatac ccgctcaatc tcctgcttgt gggcgttgtc tatgggaatc agtcggcagt
    26401 gatcgaaacc gtcatcgtcg acgaactccc gatgccggtg caggcgttgc acgaggacga
    26461 ccagctgcgt ggcgaccttg aaacgttggc cacgaacgcc gaggcctgca tctatgcgct
    26521 caacgacctc gacgccaacc tccgtcgctc gatgggcggt gatcccacgc cctgggacaa
    26581 ggggcaacgt cccggggatc gcctagcaat ggctcttgat gacgctgcgc ggcgagtgtt
    26641 gcgcggcatt cagagagagc ccgagagggc cgacgagggg ctgctggcat gggagcactt
    26701 tgtactggct cgcgcgtggg agatcgccaa ccccctcctc gacgcttgct ccgacaccgc
    26761 cttcctgggg cgtccagacc cgcgccacag ggaccggttt ctccggcagt ccacagccga
    26821 agccttcttc cgcaggtccc ttcgactagc cctggtgcga gcgcacccgc aagagacaaa
    26881 ggagtcccaa tgagcacagc taccgctgga tcgcttccag cacgccacta ctgggatcgg
    26941 ctcgttgacg accagggtcg atggagggac ggtgcgccac caggggaggc gctctcggca
    27001 ctccggcgcg ggttcgattc cgaaccaggg agcgtccctg gaatgtggcc gttctacacc
    27061 accctcacgg gcgacgggcg agtgactgcg tcgctacgcg ccgaacatct ggcgttggca
    27121 atgttcgggt tgcaccagca gagtcagtcc gcgccgatgc atcgcgctgg tgacgggctc
    27181 ggcaaggcga tggcccgctt gagggcatcc gaacgctaca gcgcggacgc gatcgatcgg
    27241 cgcttcaccc aagccgcgac ggcgacggat ctcgacgagt tgggacatca cgtgaggggg
    27301 ctgaccacga tgctcaagac gatcgccccg ccgcagccca tcgactacac gcgcctcttc
    27361 caggatctgt gcgactggca acgccctgac ctcgtcggtc gagtccgccg acgctggggc
    27421 agcgactact tccgtttgcg ctcgaaccct gctgaaccag gggacccccg cccatcaact
    27481 gcctcatccg ccggaaagga ccgctcatga ccagccccac cctctacgtt gacgtgcaca
    27541 tccttcagga cctgccgccc tcaaacatca accgtgatga caatggcacg cccaagcaag
    27601 ccgtctacgg gggcgtctct cgcctccgtg tgtcgtcgca agcatggaag cgggcagtgc
    27661 ggctcgagtt cgcggcagac ctgccgaaag aggccaccgg cgttcgcacc cgtcgcctga
    27721 cccagctgtt cacggccgaa ttggctgagc gcggactccc tgagtcagac gcaaccgtcc
    27781 tcgcggctga ggcggtcaag agcctgggca tcacagcggg aaagaagggc gatctgtcct
    27841 atctactgtt cttcagccgc ggccagctgg acaccatggc ctccgcggtc gctgcagctg
    27901 ctggcgactt gactcagtcg aagaccatag acaaggcgat gctcgcactc ggcctgaagg
    27961 agacgctccg tgccggtcat tcactggatg tagccctctt cgggcggatg gtggctgacc
    28021 tcgccgatct caatgtcgac gctgcctcgc aggtcgccca cgcgatttcc acgcacgcag
    28081 cgaagactca gttcgattac ttcacagcgg tggatgatga acaggaagag gccgagacgg
    28141 gtgcgggcat gatcgggacc gtcgagttca actctgcgac gatgtaccgc ttcgccacag
    28201 ttgccgtccc gcaactggcg gcaaacctcg gcgaccatgc agcggcgttt gacggcgtcg
    28261 ctcgattcat cagggcattc gcactctcga tgcccacagg gcaccagacg agtttcgcgg
    28321 cccgcactcg cccggccctg gtcctcgtcg tggttcgaca ggatcagcca gtgaacctcg
    28381 tgagcgcctt cgagcgacca gtgtggccgg gccagcaagg cgttatggcc ggatcccagg
    28441 aggcactggc tgacttctac caaacggagg tgcagcgctg gggcgatcaa ccggcgctgg
    28501 ttgtggcgag ctacaacgca gtcgatgaca aggggcacga gaagcttgtg gctgcctttg
    28561 gggccagccg tccgttggat gaggtcatcg aagctgtcgg ggacgtcctg cgcggtttgg
    28621 gacgtgatca tgactgatcg atcgctggtg ctgagactgg ccggacctat gcagtcgtgg
    28681 ggttcgtcga gtgagttcaa ctggcgtcag actgacgccc aaccaacgaa gtcgggcatt
    28741 gtcgggctgc ttgctgcggc ttcgggccgg cggcgggaag accccatcga ggacctgctg
    28801 gcactgcgtt tgggcgtgcg catcgaccaa ccaggcaccc tattgcgcga ctatcacacc
    28861 gtcagcgact ttcgcgggac ccccttgcca tcggctgcgc tcaacgcgtc tggacgtcag
    28921 aagcccacat cgccaaagaa gttcacgcat gtgacccagc ggtactacct ccaggattca
    28981 gtcttcgtcg ctgtcattgg cgggaacacc caggtgcttg agggtctagc ctcagcgctt
    29041 cgacgtcccc gcttcccgct cgcgctgggt cgacgtgcgt gcgtgccgac ccaacccatc
    29101 gtcctctcgg agggcgccgg tgccgatctg tggcggggga ccgttgccga ggtgatccat
    29161 cgcgtgcctt ggcaggctgg gcctgcttac cgggtgtcag ccgccgatag ctcgaccgtg
    29221 acagtgccgt tcacggtcga tgacgacaac ggcgacgacg acctggccga cgttcccttg
    29281 tccttctccc gcgagcagag ggctaagcga tcaacccgcc gagttcggca cggctgggcc
    29341 gagctggggt cgggctcggg cgcgaccaac gctcaggcgc cgcagccggc tcgacacaat
    29401 ccctttgcgc tcttggggtg gtgaccatgg cctacctgtc actcatcacc ctgaacccac
    29461 ttcgacgcga aacgcagcga ttgatcagca gcccccaacg tctgcatgcc gccgtccttc
    29521 ggggtctccc tcaggatgac gtcaccgaga gagtgttatg gcggcttgag cgccgggagc
    29581 gaaccatgga ggtgctggtg ctcacccaac gtcgcccatc ctgggcggca ctggtggagt
    29641 ctgctggctg gccgggcgcc gacggtggcg agggcctagt caaggactat gaccccttgt
    29701 tggctcgggt cgccgttggg cgcgagttct catttcgggt cgtcacaaat ccgacgtcca
    29761 ccctcagcgt gctcaaaagc ccgaccgcac agcaacgcgc gtccctcgat gacgcgtcgc
    29821 ggaagcgcgg catacgtgta gggcatcgca cggccggaac tcagttgggt tggttcttgg
    29881 cgcgggccgc cggcgacttt gacacgtggg gtttcacggt gggggtgccc ggcgacgctt
    29941 ccgtcgaact agtggctcgt agacatgaat ccttcatcaa gggcgtcgat cggcgtcggg
    30001 tcaccctcga cacggcgacg ttcgaggggc gcctcaaggt cactgatact gagcgcttca
    30061 ccgcgacgct gttgggtggc ctcgggcgtg ggaaggctta cggctgcggc ttgttgacgc
    30121 tcgccccgcc tcgggaggct gcgcatgtgg tggaaggctg agactcagag cctgcaccgc
    30181 gtgagtgatc gagtctcgac cgtctatgtc gagcgttgcc acgtcgaccg tttcgagaac
    30241 ggcatcgtca tcgtcaacaa ggaacgcacg gttcgggtgc ccgcggcgta cgtcgcagca
    30301 gtgctcctcg gccccgggac tcgcatcacg cacgcggccg ttgcgcttct cgccgactca
    30361 gggaccgtgg tggagtgggt cggcgagcac ggagtccgga tgtatgccgc aggtttaggc
    30421 cccagccgcg gctcgcaagt gatcacccgc caggcctacc tagtaacccg cactcaggag
    30481 cgactcgggg ttgctcgggc gatgtacgcc atgcgctttc ccggagaaga tgtcgccgga
    30541 ctgaccatgc agcaactgcg tggcagggag ggtgcccgcg tcaagaagat ctataaacgc
    30601 gaagcccaac gaaccggcgt ggcttgggcg actcgcgagt atcgggcggg
//
LOCUS       DAORPW010000012        29098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_243_length_29098_cov_812.209345, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000012 DAORPW010000000
VERSION     DAORPW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 29098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29098
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_243_length_29098_cov_812.209345"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /gene="der"
                     /locus_tag="PLB86_02155"
     CDS             <1..920
                     /gene="der"
                     /locus_tag="PLB86_02155"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRC63969.1"
                     /translation="GLGLGQPWPVSALHGRGSGDALDALMAVLPERSAVGGAYERGGP
                     RRVALIGRPNVGKSSLLNKVAGSERVVVDSVAGTTRDPVDELVEIAGRQWRFVDTAGI
                     RRRVHQTRGADFYASLRTQTALEKAEVAVVLIDAGEPISEQDIRVIQQVIDAGRALVV
                     AYNKWDTVDEERRHYLEREIEKELVQIPWAPRVNVSARTGRHLEKLVPALEVALDSWD
                     SRVSTARLNAFLGEIVAAHPHPVRGGKQPRILFATQASTRPPRFVLFASGFVEAGYRR
                     FIERKLRERFGFEGTPIEVSVRVREKRKR"
     gene            1038..1111
                     /locus_tag="PLB86_02160"
     tRNA            1038..1111
                     /locus_tag="PLB86_02160"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1072..1074,aa:Pro,seq:ggg)
     gene            1366..1599
                     /locus_tag="PLB86_02165"
     CDS             1366..1599
                     /locus_tag="PLB86_02165"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRC63970.1"
                     /translation="MSLLERITSDPAVCHGQPTVRGLRYPVQTLLELLSSGMTIDEVL
                     DDHPDLEREDLLAALEFGALASGGHRVFPRGAA"
     gene            1596..1955
                     /locus_tag="PLB86_02170"
     CDS             1596..1955
                     /locus_tag="PLB86_02170"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRC63971.1"
                     /translation="MRFLVDAQLPARLAEALTAAGHDAVHTSQLPDGNRATDHQVAEA
                     ADSSDRVVVTKDSDFRDGHLLAGRPRRLLVVGTGNITNNALLALFDASLVDIVEAFEE
                     ADFIELQTDSLIVHPRR"
     gene            2115..3095
                     /locus_tag="PLB86_02175"
     CDS             2115..3095
                     /locus_tag="PLB86_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRC63972.1"
                     /translation="MRAVVIEAPGPPEVLSVRSVPLPVPQDGEVLIRVMAFGLNRSEL
                     HFRQGVAESGSFPRIPGIEAVGVVESAPGGTFAVGAQAATLMGGMGREFDGGYAEFVK
                     VPAGQVIPFTSHLSWATLGAVPEMLQTAAGSLDVGLQAETGQTVLIRGGTSSVGMAAA
                     VLAKLRGLTVISTTRNPERVGSLRSLGVDHVLIDDGQIAAAVRDLMPGGVDCAVELVG
                     VNVLKDTLLSTRAGGTVCFTGMLSDEWTIRDFYPMDWLPNGVRLTAYSGGAADLTPQT
                     LQGFLDAVAAGSAVVPVGRIYRLDEIVQAHHDMQNNVVGGKGVVLTDKAG"
     gene            3275..3685
                     /gene="arr"
                     /locus_tag="PLB86_02180"
     CDS             3275..3685
                     /gene="arr"
                     /locus_tag="PLB86_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HRC63973.1"
                     /translation="MTGVLDPGPFFHGTKADLQVGDLLTAGFRSNYRPEVLMNHIYFT
                     ALTDGAGLAAELAAGDAAPRVYIVEPTGPFENDPNVTDKKFPGNPTRSYRSVEPLRIV
                     GELSDWVRLTPEALQEWRDRLAAILADERGVIIN"
     gene            3766..4371
                     /locus_tag="PLB86_02185"
     CDS             3766..4371
                     /locus_tag="PLB86_02185"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRC63974.1"
                     /translation="MEERRDIAGDETPDTDRVLTVPNALSVLRLVGVPLFLWLLLRGD
                     DGWALVTLALSGITDYLDGKIARHFHLVSRVGQLLDPIADRLYIISTLLGMAWRDIIP
                     WWIVGVLLLREAFMAVLMLALKRVGQIGLPVHFVGKGATLNLLYAFPVILLGQVPGTV
                     GEWALPIGWAFAWWGIVLYWVAAALYAAQAAPILRARGRVA"
     gene            4368..5405
                     /locus_tag="PLB86_02190"
     CDS             4368..5405
                     /locus_tag="PLB86_02190"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HRC63975.1"
                     /translation="MSPRRVDESMALITELREGSLEAGYAAASARRVAEGQPSHTSTR
                     SPLVIAFAVIVGFLLVVSAMALRSATSVVEQARAELIGQITARQEAGDALAEKVSALE
                     AEVAGARSAALGASGQSGVLSQLAFLQSVTGVVAVTGPGLTVTLDDSADAVSGADAAR
                     GSSGFEAGRVSAADLQIVSNGLWAAGAEAISINGQRLTTRSAIRFAGQAILVDFRPLV
                     RPYVVTAIGDAKALQSSFGSSPTGSYLKSLEDNYGIPVAISGSASLTCPAGVPSALVS
                     ATARPTGIPSTTAAPTSTATSTRSTSTTTSTTKTSTSKTSTAKTSTTKTATSPITRVT
                     TSSPSTTEIRP"
     gene            5402..5734
                     /locus_tag="PLB86_02195"
     CDS             5402..5734
                     /locus_tag="PLB86_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HRC63976.1"
                     /translation="MIPAIGLFVGVVLGLLLHPSVPLWLQPYLPIAVIAALDAVFGGV
                     RAVLDGIFVDKVFVVSFLSNVLVAAFIVFLGDQLGVGSQLSTGVVVVLGIRIFSNVAA
                     IRRHLFKA"
     gene            5737..6549
                     /locus_tag="PLB86_02200"
     CDS             5737..6549
                     /locus_tag="PLB86_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HRC63977.1"
                     /translation="MAVRRPRRRPGSKAGVVTREPEEPREVTWRRLRRMFGARATRAN
                     VLATVLTLALGFAIATQIQQNQEGGLATLRQDELVRVLDDVTQRSSRLDAQVRELEVQ
                     RNALQSGADRAKAATEQAQERLDSLAILAGTVKATGPGIRVTIADSSGAVRASALLDV
                     LQELRDAGAEVVQFGTVRIVAGSYFTDAGTSITADGQSLSRPYVILAIGDPQTLASAM
                     NIPGGIVDSMKRLGASASVDQLSTVTIDALHTPKTPRYAQPVPEPTVAPTGG"
     gene            6645..7034
                     /gene="gcvH"
                     /locus_tag="PLB86_02205"
     CDS             6645..7034
                     /gene="gcvH"
                     /locus_tag="PLB86_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRC63978.1"
                     /translation="MSELSYPEDLRYTSDHEWVAVASDGTARVGITSFAQNALGDVVY
                     VSLPAVGATVSAGDACGEVESTKSVSDVYSPLDGVVTSVNVTLDTAPELVNSDPYGDG
                     WLFEMRPSDPEAVAHLLDAAAYQAVLG"
     gene            7335..7721
                     /locus_tag="PLB86_02210"
     CDS             7335..7721
                     /locus_tag="PLB86_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRC63979.1"
                     /translation="MRFRGLGEIPEAFDPDGGLSAEDEATIGALRHGTALLVVVRGPN
                     NGARFLLDDAEVTSGRHPESDIFLDDVTVSRRHAVFVRDGNGYAVKDVGSLNGTYVNR
                     ASVDAAVLRNGDEVQIGKYRFLYYSR"
     gene            7732..8442
                     /locus_tag="PLB86_02215"
     CDS             7732..8442
                     /locus_tag="PLB86_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRC63980.1"
                     /translation="MAARRRGLSIGLVLERLQGEFPDLTISKLRFLETEGLVAPERAP
                     SGYRLYTEEDVRRLRYVLTAQRDRFWPLKVIRDALDAMDRGLTPATDTSGERPAVPTA
                     AADPDVPAAATLAAPPSLRLTEAELMGAAALDEAMFQALRGFHLLRPDATGHYDDAAL
                     GVARAAAALAAYGVEARHLRPFRTAAEREVGLVQQIVVPALASAGGRASGADPTAEVL
                     HHCLALHAALVRAELRRG"
     gene            8476..8997
                     /locus_tag="PLB86_02220"
     CDS             8476..8997
                     /locus_tag="PLB86_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRC63981.1"
                     /translation="MIEVDVLGVRVEMPTNQPIVLLRERDGERYLPIWIGAAEATAIA
                     YAQQGVVPPRPLTHDLLKDTLEALGHALVRVRIDELRDKVFYATLVVDGTEISARPSD
                     GIALALRTASPILVHEDVFTAAGITMAPEEDDEVERFREFLDQVSPEDFEAPDGPGGL
                     GGSEGERPPSPEQ"
     gene            9163..9747
                     /locus_tag="PLB86_02225"
     CDS             9163..9747
                     /locus_tag="PLB86_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRC63982.1"
                     /translation="MSAIAPPSRPAGVGPAQGLLFDDDLPELREDEGYRGPTACRAAG
                     ITYRQLDYWARTGLVEPSVRSATGSGTQRLYGFRDILVLKVVKRLLDTGVSLQQIRQA
                     ISHLRERGVEDLARITLMSDGASVYECTSADEVIDLLQGGQGVFGIAVGRVWREVEGS
                     LSALPSERAEDDEASAPVSAGDELSARRASRMMA"
     regulatory      9864..9974
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10049..12973
                     /gene="gcvP"
                     /locus_tag="PLB86_02230"
     CDS             10049..12973
                     /gene="gcvP"
                     /locus_tag="PLB86_02230"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRC63983.1"
                     /translation="MPQHPATPSTALPAFVGRHIGPGEADVAHMLAVVGQPSLDALVD
                     SALPHLIRSDAPLALAAADSEAAVIAELRALAGRNRVLRSMIGLGYYGTISPPVVTRK
                     ILENPAWYTAYTPYQPEISQGRLEALLNFQTVVSDLTGLPIAGASLLDEATAAAEAMT
                     LMRRSTRLPDDAVLVVDTHTFPQTLAVVQTRARPLGIGVVVADLSTVTTGEQLRAAAG
                     GVPVFGVLVQYPGADGQVRSLSALAAAAHEIGALLTAATDLLALTILAAPGEWGADIA
                     VGSAQRFGVPMGFGGPHAGFMSVRAGLERSMPGRLVGVSVDAHGAPAYRLALQTREQH
                     IRREKATSNICTAQVLLAVIASMYAVWHGPEGLAAIARRVNAHARTLARGLSAGDIEV
                     LTSAPFDTIRVRVPGRAHKLQREAAARGLNIWVVDADTLSLSCDETTTPEDLDTVFDA
                     FGIAAPGAAPEGSDGPEGADGADGVGWDASLSRSTAYLTHPVFHTHRSETQMLRYLRR
                     LSERDYALDRGMIPLGSCTMKLNATTEMEAITWPEFADLHPFAPTNQTEGIRALIGQL
                     GDWLTEITGYDAVSLQPNAGSQGELAGLLAIHAYHEARGEGHRRVCLIPASAHGTNAA
                     SAVMAGLKVVVVKTAAGGDIDMDDLRAKVEAHGPELAAIMVTYPSTHGVFEETITDLC
                     ALVHDAGGQVYVDGANLNALVGLAQPGRFGADVSHLNLHKTFCIPHGGGGPGVGPVAV
                     RSHLAPYLPNHPLAPECGPSTGVGPVSAAPYGSASVLPISWAYIRLMGGAGLTRATQV
                     AILNANYIAARLREHFPVLYAGASGLVAHECILDLRGITAETGVTVDDVAKRLVDYGF
                     HAPTMSFPVAGTLMVEPTESEDLGELDRFIAAMISIRDEINAVRDGVITAEASPLRSA
                     PHTAYALTGEWDRAYDRTTAVFPPGVDPTDKYWPPVARIDGAYGDRHLVCSCPAPEAF
                     ED"
     gene            13074..15164
                     /locus_tag="PLB86_02235"
     CDS             13074..15164
                     /locus_tag="PLB86_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRC63984.1"
                     /translation="MGKKHRREQKLAKQLAKELAKDEQRVGSPIPESLRPRPALSYEL
                     DEAKESVALSALRRVTPGLTYLLTQYPRGMIRRDAVAGVAVAAYLIPQVMAYSAIVNV
                     PPVASLWTALAAIIAYAVLGSSRILSSGPESTIALMTGVAIAPLAASNPDRAVALSAA
                     LALCVAGWCLIARVLRAGIIAELLSTPLLVGYLAGGAVLMIVGQLGKVTGTSVDGESI
                     VEQLQSFFSVVGRTQMPTLIVGGATLALILVIITFRPKWPAPLIAVVLATVASVLLHL
                     EAQGVKVVGTVPSGLPMPHLPMVTGEEFKAMIWAGLGIAIVGYSDIMLISRGFPLTPG
                     EGETKADVRADPQQELVAMAGVHAFVGFFSGYPVSASGSRTALAVAGGARSQAYSLVA
                     GLCVVAVLFFAGPLMAGLPSAALGAVVFYAAGKLVSIPDFKRLWAFRRREFFLAIIAM
                     LGTVVIGILQGVLFAIALSLLEMLHRLARPHEGVLGRVPGIPGMHDVADYPDARTIPG
                     CIFYRYDAPLFFANVGDLRERVDRVISVENQAYPQTPVRWFILNVEANVEVDITAADA
                     LRELAQELADRGIELGLARVKFDLYEPLDRAGVIDVIGKDMLFATLPVAEEAYLKWAV
                     AQANSGSVEPDLEVVQAAVDSVPAHSLLPWDRPDDEEDDDGPRPNLGPAEARVAVLDP
                     KTAGPDQPADGVAR"
     gene            complement(15165..18197)
                     /locus_tag="PLB86_02240"
     CDS             complement(15165..18197)
                     /locus_tag="PLB86_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HRC63985.1"
                     /translation="MRLHRLQARAFGPFAELIEVDFDELADAGIFLIHGATGSGKTSL
                     LDAVCFALYGRVPGLRPAGETLRSDHAGADDVPEVTLELTVGNRRLRVTRSPDHHRPK
                     KRGNGLTPVRGQVRLDELSRDGVWTTLATRADDVGDSLGQALGMGLDQFAKVVLLPQG
                     DFAAFLRATPEERRVLLERLFDVSRYTGVEAWLTDRRRLTAATAAEARATLSADLLRV
                     DDVLARVPAEVIDGLPTWAEATETGRLAEIAAEASTRVGAHALAALTAAATAEQAASA
                     AESAHAAAALTARAAARGEAARRVLAEFAAGSDARAAAARRLELGERAGRVAGDLRAL
                     AAAEAERDSAHTRYAASCTALARWSVDVGDSPQEALSRARGLDASLDVLAQRGAAEAT
                     RTQRQASVFVQREVAQRALATIDAAEAALVEQVAQLQVRADALLTIAAGSGQAEASAS
                     QAGRLVTLRDDLDRTAITIASLESAGLAATRVALAQAGRLLDLRQRRLDGMAAELAAG
                     LHPGDECPVCGGTEHPAPATAVDAVTAADVDAAEEAWRPLDDRVRRIETDLAAARAHH
                     DLLVEQLGGDERDLAILRDAADVIGRAVAVAASAVAELPQVQAELAQRRQALETLAES
                     RRAALGQLAAAQALEGELAEAAEHDAAVVRELVARHTEGCPCADPQVPTADVPARHRR
                     VVAALADTAAARSASDEAQRRHTEAHEATSSVCRAEGFADVVQACAAAVPAATLVEIR
                     EGIAAKVAAAAAARATVADPEVERALGAPLPDLAVLAAMMNAARGQRTAAAREHALAE
                     QAARDIDALSRQVAASAAAATAATGVATSTAALADVVSGLGSDNTLRMKLSAFVLAGR
                     LERVVELANERLQRMGDGRFHLAHSDRLASGGRRSGLGLVVQDEWTGQVRDTATLSGG
                     ESFMASLALALGLADAVREESGGIDLHTLFIDEGFGSLDGESLEQVMEVLDQLQDGGR
                     AVGVVSHVPALRDRIPAQVQVDKTRRGSTVRRVTGPAA"
     gene            complement(18194..19369)
                     /locus_tag="PLB86_02245"
     CDS             complement(18194..19369)
                     /locus_tag="PLB86_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HRC63986.1"
                     /translation="MRIVHTSDWHLGRSFHQVGLLGAQVQFVEFLVDLVRTEGVEAVL
                     VSGDVYDRALPSPDTVSVLADALTRLVDAGAQVVLTSGNHDSAIRLGFASDLLARAGV
                     HIGASLSSVGTPVVLGDVAVYPLPYLEPAVVGPALGVGERSHAAVLRAAMDRVRVDLT
                     SHDLPSIVMAHAFVAGGTTTESEREIAVGGVSVVPADVFNGVTYAALGHLHRPQWVRD
                     DVAYSGSPLAMSFGEADQIKSVSLLDLTATGVSVEAIPTPVPRRLAVLRGDLADLLTN
                     PSLEWARGAWCRAVLTDPVRPLAAYEQLTRRFPDLLHLEFAPRGGELHATSRYAARVP
                     SARAELDVCCDFLAHVRAGAAASDDERATLAEALDASRAARAEADDEGVADQSRGVA"
     gene            19478..>20594
                     /locus_tag="PLB86_02250"
     CDS             19478..>20594
                     /locus_tag="PLB86_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRC63987.1"
                     /translation="MGQDIESITYTREQRQRFRQKVHLCLDVFERMLAYSQFDFERPM
                     TGMEIELNLTGSDFRPKMDNALVLERIADPEYQTELARYNIEFNVKPRELGGDSMLSL
                     EGDLRASLNRAHETSAEVGSKIVMVGILPTVTLDDFAGDWMSANARYHALNDAMLGAR
                     GEDVHLDIEGPTGEHLERYFDSLAPEAACTSMQLHLQVAPLSFADHWNAAQALAGAQL
                     ALAANSPFFLGKRLWAESRIPVFSQAVDTRSPELKNQGVRPRVWFGERWITSIFDLFE
                     ENVRYFPALLPETTDEDPSAVLDAGGVPALAEMRLHNGTVYRWNRPIYDIGGGHPHVR
                     VENRVLPAGPTIVDTLANAAFYYGALRMLAQEDRPVWS"
     assembly_gap    20595..20694
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20695..21114
                     /locus_tag="PLB86_02255"
     CDS             <20695..21114
                     /locus_tag="PLB86_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRC63988.1"
                     /translation="FYYGALRMLAQEDRPVWSRMSFAAAEDNFLAGARDGLEAKLYWP
                     GRGDLPATELILRHLLPLANEGLRDWGVSTEARDRYLSVIEGRCLTGVNGATWQTATV
                     AALEERGIPREEALRQMVATYSEHMHANTPVHTWPMP"
     gene            21111..21983
                     /locus_tag="PLB86_02260"
     CDS             21111..21983
                     /locus_tag="PLB86_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRC63989.1"
                     /translation="MTRAFRLLNVFALADDPFSGNPLAVIEDASGLDGATMLAIARQF
                     NLSETTFVTAGGDTSGAGPADATVRIFTPGYEMPFAGHPTLGTACVVGERLGKNHVVL
                     RVPAGDIPVDGLSVPHSGDQTWTLTAKPATITEGAVARSDLASAVGLNADDLVDEGWW
                     VDAGVDQFLTEVTSVQAVRRAAADPVAARRFILSPRGESMALVWAWTSPDTIEARFFF
                     SQDAAMLEDPATGSAAANLGSLLAHRGFRGAVTVDQGAAVARPSRLHIEVTEAGRVRV
                     GGLVREIGRGTLAL"
     gene            21983..22516
                     /locus_tag="PLB86_02265"
     CDS             21983..22516
                     /locus_tag="PLB86_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HRC63990.1"
                     /translation="MPTYVAFLRAVNIGRRQTPMARVRATLEASGFTDVQTYIQTGNV
                     LLTTTARSPEKVAQRVREALSGDFGFDIPVVVRRPRDLVDLVAAVDALPDPFPQGRTY
                     AAFLDQEPSDAATAVLNDWSAPGEWVRVVGRHVVLRLGIPANVVTLTNAKIERFGVVA
                     TTRDLTVVRALATRWGG"
     gene            complement(22476..23231)
                     /locus_tag="PLB86_02270"
     CDS             complement(22476..23231)
                     /locus_tag="PLB86_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRC63991.1"
                     /translation="MTIYGRNPVLEALGDPSLTVDKVLMADSAHGPHVSEIRSAARAR
                     GVPVQLVGAERVKKIAGNGKQDQGVCADVVAPRMRPLDLALADGLRGPIIVLDGITTP
                     ANVGMILRTATAAGFSGIVVPRRGVASIDPLVVKASAGVAFKAPILKVATAVEAVRLL
                     LGAGYRIAGLDADGPADLFAADLPGDTAYVMGGETAGLGAEVSELVDTWVSIPMAGGV
                     ESLNVSAAAAVVCFDVVRRGLNRPSGSPERARR"
     gene            23456..25402
                     /locus_tag="PLB86_02275"
     CDS             23456..25402
                     /locus_tag="PLB86_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HRC63992.1"
                     /translation="MTSTAPAAVVDATASILADLSPERRELFELRLHRWWDDLLDALE
                     TLYGERAFDVAQRLVAHAAAAYRDRDPELHRLDERRILAPDWLQDPRMFGYATYADRF
                     AGTLEAVGGKVDYLEELGVTYLHLMPLLRPREGDNDGGYAVASYRELRPDLGTIEDLK
                     ALSKLLRDKGISLVLDLVLNHVAKEHDWAQRARAGESHYRDYFYVYPDRTEPDAYEAT
                     LPEVFPDFAPGNFTWDDAMGGWVWTTFNSWQWDVNWANPDVFCEYADIIFFLANAGVE
                     VVRLDAIAFTWKRLGTNSQNQPEVHALTQALRAIARIACPAVAFKAEAIVGPRDLVQY
                     LGQGEHHGKVSDLAYHNSLMVQAWSMFATQDVRLTAHALSALPPVPTTATWISYLRCH
                     DDIGWAIDDGDAAAVGLNGFAHRAFLSDWFIGAYPGSSARGLTFQANEATGDRRISGT
                     AAALTGLDAAREVGDQAAVDRAISRILLGHALIAGFGGVPVIWSGDELGLPNDPVWAG
                     EPGHEDDNRWVHRARMHWEVAERRHTPGTPEHRVFGGIAHIAHTRARLPHLHASVPSS
                     VLGVWDSGVLVFARRHPVGTMLAAYNVTDSWRPVPGWVLRQEGLSSGTEALSGAHVRI
                     EADDNAWMPPYAVWWLVDHIPAHA"
     gene            25412..25798
                     /locus_tag="PLB86_02280"
     CDS             25412..25798
                     /locus_tag="PLB86_02280"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HRC63993.1"
                     /translation="MQQSVSFKVYYEDTDSLGVVYYANYFKFMERGRSEFLTAQGRSV
                     ADWNAAGFLLVVHSVQATFKRPAVLGDTVDIVTSFTLPSVYRGRFAQRVERAGELLVD
                     GIVDVVCLDAAQNLIEFPQQLRALAD"
     gene            complement(25828..27087)
                     /locus_tag="PLB86_02285"
     CDS             complement(25828..27087)
                     /locus_tag="PLB86_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRC63994.1"
                     /translation="MQTLDIDHLGTVIDSRRTDLRVVTSGNCSTPLTVLGKVDTLLAE
                     YRLHLLNAPRGIPQRDGVIPESAFVGVGMRHHPRLVYFPMRLSGVPFLYQRRLRVDVV
                     IAHTSAPIAGKVSLGLEINVLPGAIAACKAAGGIVIAQVNRQMPYTFGDGELPLDVFD
                     AIVEVDEPVLNLATSEADGTASGGGMSRADSARIIGDLVSRRVADGATLQLGIGEIPD
                     AALDGLRGKRGLGIWSEVIGDGVLELDQAGALDPARTITASALMGSAALYAWAHRNER
                     LRVLRTETTNSPSIIATQPLMTSINTALQVDLYDQANAASVNAKVYSGFGGQSDFTVG
                     ALHAPRGQALMALRSWHPKADVSTIVPLIEAPVTSFQHTAVITEQGVAEIAWRDQKSQ
                     AVQLIEHAAHPAARDDLWEEAQELGLL"
     gene            complement(27092..27790)
                     /locus_tag="PLB86_02290"
     CDS             complement(27092..27790)
                     /locus_tag="PLB86_02290"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HRC63995.1"
                     /translation="MDHTLTAGARRWLALNAVLAWLGLAVSLTLTVTNIYPSTVTVPT
                     RYGADHAAGLGSRLADWFSYFTIWSNIVVAVVTSLLAWGRPRVGAALRAARLDSVLLI
                     TITGLVYAVVLAPVSVQRGWENVSNTLLHQVTPVLTVLVWVLVGPRGWVSRRTVVHSL
                     AIPIAWLVVMLLHGAFVGAYPYPFVDVAVLGYARALGNVAGILVLGLLMAGGFLAAER
                     GLARHWRRAPSLRE"
     gene            complement(27818..28972)
                     /gene="rlmC"
                     /locus_tag="PLB86_02295"
     CDS             complement(27818..28972)
                     /gene="rlmC"
                     /locus_tag="PLB86_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HRC63996.1"
                     /translation="MQCDYYDAGVCRSCTLMGTAYSQQLAAKDARCRDALSLIAPQLD
                     WLEPFASAEHGFRNKAKLVVGGTASAPTLGILDGHGRGVDLRDCGLYDPALHAALPHL
                     ADFVIDAQLTPYDVPQRRGELKYLLVTASPAGALMLRIVLRSNSQLPQARSALPRLQA
                     ALPHLQVVTVNLHPQHKAVLEGDTELVLTKQSELSMPVADAELHLGPRSFFQTNTAVA
                     AGLYRQAQAWIEAAQPASVLDLYCGVGGFALQAAAAGRHVIGVEVSADAIAAAQRSAA
                     GLLAQRPDCGPVDFVVGDATPYAIAHPAPDLVVVNPPRRGLDETLTSWLESSTVRDVV
                     YSSCNVTTLARDLARMPSLIPHQARLFDMFPQTDHHEVMVRLRRSPNAHA"
BASE COUNT         4334 a        10148 c        10028 g         4488 t
ORIGIN      
        1 gggggctcgg gttggggcag ccgtggccgg tctccgccct gcacgggcgg gggagtggcg
       61 acgcgctgga cgccctcatg gccgtgctgc ccgagcggtc ggcggttggt ggggcctacg
      121 agcgtggcgg gcctcggcgg gttgccctta tcggtcggcc caacgtcggc aagtcgtcgc
      181 tgctcaacaa ggtggctggc tcggagcgcg tcgtcgtcga ctcggtggcc gggacgacgc
      241 gcgacccggt cgatgagctc gtcgagatcg ccgggaggca gtggcggttt gtcgacaccg
      301 cgggcatccg gcggcgggtc caccagacgc gtggggccga tttctatgcg tccttgcgca
      361 cccagacggc gctggagaag gccgaggtgg ctgtcgtgct catcgacgcc ggtgagccga
      421 tctctgagca ggacatccgg gtcatccagc aggtcatcga cgccgggcga gcactggtcg
      481 tggcctacaa caagtgggac acggttgacg aggagcggcg tcactacctc gagcgcgaga
      541 tcgagaagga gctcgtccag atcccgtggg cgccccgggt caacgtgtcg gcgcgcaccg
      601 gtcggcacct ggagaagctc gtcccggcgc tcgaagttgc tctcgactcg tgggactcac
      661 gggtgtcgac ggctcggctc aacgcgttcc tcggggagat cgtcgcggct cacccgcacc
      721 cggtgcgtgg cggcaagcag ccccggatcc tcttcgccac gcaggcctcg acccggccgc
      781 cgcggttcgt gctcttcgcg tcgggcttcg tcgaggccgg ctatcgccgg ttcatcgagc
      841 gcaagttgcg cgagcgcttc ggcttcgagg ggacgcccat cgaggtgtcc gtgcgggtcc
      901 gcgagaagcg caagcgctag gcgggtgccg gggcggctgc cggttgcgcg gcatacggat
      961 tgggagcccc ggtatgccgt gcggtagggt ttcgttcgct ctcgtggcgc ccttttgggg
     1021 cggtgggagg gcagccacgg gctgtggcgc agcttggtag cgcacttgac tgggggtcaa
     1081 ggggtcgcag gttcaaatcc tgtcagcccg accgaatcgg ctctgatctg cctacgcagg
     1141 tcagagctgt tctttgtgcc ccggcgcggc cgccgtcggc gacaccgaac cgacgcgatt
     1201 ccgcaccacc aggtcgggaa tgcggtgccg gcgtcgatca ccgcctgggt gtccggtgca
     1261 catagcggct cccagtgagt ccgaggtgcc caaccgtgac cgcccgcctc tgggttccgt
     1321 tctctgctcc ggcggtggac accctggtcc cgatattctg ggcacatgtc acttctggag
     1381 cgcatcacct ctgacccggc ggtctgtcac ggccagccga cggtgcgcgg gctgcggtac
     1441 cccgtccaga cccttctgga gctcctgtcg tcgggcatga ccatcgacga agtcctggac
     1501 gaccacccag accttgagcg tgaggacttg ctagccgccc tcgagttcgg tgccctggca
     1561 tcaggcggtc accgcgtgtt cccacgcggc gcggcgtgag attcctcgtc gacgcccagc
     1621 tgcctgcccg ccttgcggag gcgctaacgg cagcgggcca tgacgccgta cacacgagtc
     1681 agttgcctga cgggaaccgc gccacggacc atcaggtggc cgaggcggcg gattcctcgg
     1741 atcgtgtggt ggtcaccaag gacagcgact tccgcgacgg ccacctgctc gcagggaggc
     1801 cccggcgcct gctcgtcgtc ggcaccggca acatcaccaa caacgcgctc ctagcgctgt
     1861 tcgacgcaag tcttgtcgac atcgtcgaag ccttcgaaga ggctgacttc atcgaactgc
     1921 agaccgactc tctgatcgtg catccacgca ggtagcaggt gagtcagaag ccgtgacttg
     1981 agagaccgat cgaccgatgt tcctgcaggt ccgagggcgg attccgcata gtctgctagg
     2041 gcaactgcac atctccatca caatctcggt aggtgagttc tcatgggccg caaggggatc
     2101 cgagcatgac cacgatgcgc gctgtggtga tcgaggcccc tggaccccca gaagtcttga
     2161 gcgtgcgctc cgtcccactt ccggtgcctc aagacggcga ggtgctgatc agggtgatgg
     2221 ccttcggcct gaaccggtcg gagcttcatt tcaggcaggg cgtggccgag tcggggagct
     2281 tcccgaggat tccgggcatc gaagccgtcg gcgtcgtcga gtccgcgccg ggaggcacct
     2341 tcgccgtcgg cgctcaggcg gcgacgttga tgggcgggat gggtcgagag ttcgacgggg
     2401 gatatgccga gttcgtgaag gttcctgcgg ggcaagtgat cccgttcacc agccaccttt
     2461 cgtgggcaac gctgggtgct gtgcccgaga tgctgcagac cgcagccggt tccctcgatg
     2521 tcggcctcca ggccgagacg gggcagacgg tgctcatccg cggtggcacc tcttcggtcg
     2581 gcatggctgc tgcggtgctg gccaagctgc gcgggctgac cgtcatctcg acgactcgga
     2641 acccggagcg ggtcggctcg ctgcgcagcc tcggtgtgga ccacgtgctc atagacgacg
     2701 gccagatcgc tgctgcggtg cgcgacctga tgcctggggg tgtcgactgc gccgtcgaac
     2761 tggtgggtgt caacgtcctg aaggacaccc ttctctcgac cagggcagga ggcaccgtct
     2821 gcttcaccgg catgttgtcg gacgaatgga cgattcgtga cttctatccg atggattggc
     2881 tgccgaacgg cgttcggctc acggcctatt cgggtggggc ggccgatctg acgccgcaga
     2941 cgctgcaagg gtttctcgac gcggtcgccg cggggtcggc ggtggtgccc gttggccgga
     3001 tctaccgcct tgacgagatc gtccaggccc atcacgacat gcagaacaac gtcgtcggcg
     3061 gcaagggtgt cgtcctcacc gacaaagccg ggtagccagg ggtgggccgg ctggattgat
     3121 tcgactggtc atgcgagtgc gcgagggagt gcgattgatg gggtgatcac ccctctgatc
     3181 gcactccgtg ccgcacgcgc aacccagaca tcactcacac atccggcatc gacgtccaga
     3241 accgcttcaa ggacgccctc gtagactgcc gcaggtgact ggcgtgctgg atccggggcc
     3301 gttcttccat gggacgaagg ccgacctgca ggtgggcgac ctcctcaccg ccggcttccg
     3361 gtcgaactat cggccagagg tgctgatgaa ccacatctac ttcaccgcgc tgaccgacgg
     3421 cgccgggctc gccgcggagt tggctgcggg cgacgccgcc cctcgcgtct acatcgtcga
     3481 gccgaccgga cccttcgaga acgaccccaa cgtgaccgac aagaagttcc ccggcaatcc
     3541 gacccgctca taccgcagcg tcgagcccct gaggatcgtc ggtgagctga gcgactgggt
     3601 gcgcctcacg cccgaggccc tccaggagtg gcgggaccgg ttggccgcca tcctcgccga
     3661 cgaacgcggc gtcatcatca actgaggagc ggtcgcgggc ggcataccgg cggggcggag
     3721 gcgaccaagc ggggatcgtc acccgcgcgg gttaggctcg gggccgtgga ggagcgcagg
     3781 gacatcgcgg gcgacgagac gcccgacacc gaccgcgtcc tcaccgtgcc caacgccctg
     3841 tcggtgctgc gactggtggg tgtgccgctc ttcctctggc tgctgctgcg cggcgacgac
     3901 ggctgggccc tggtcacgct cgccctctcc ggaatcaccg actatctcga tggcaagatc
     3961 gctcggcact tccacctggt ctcccgcgtg gggcagttgc tcgacccgat cgccgatcgc
     4021 ctctacatca tcagcacgct gctgggcatg gcctggcgcg acatcatccc ctggtggatc
     4081 gtcggggtcc tgctgctgcg cgaggccttc atggccgtgc tcatgctcgc cctcaaacgg
     4141 gtcgggcaga tcggcctgcc ggtgcacttc gtcggcaagg gcgccacgct caacctgctc
     4201 tacgccttcc ccgtcatcct cctcgggcag gtgcctggga cggtcgggga gtgggctctg
     4261 cccatcggtt gggcgttcgc gtggtggggg atcgtcctct actgggtcgc cgccgccctg
     4321 tatgccgccc aagccgctcc cattctgcgc gcccggggga gggtcgcatg agtccccgcc
     4381 gggtcgacga gtcgatggcc ctcatcaccg agcttcgcga ggggtcactc gaggcggggt
     4441 atgccgcggc gtcggctcgc cgggtcgccg agggccaacc gagccacacc tccacccgca
     4501 gtccgttggt tatcgccttc gcggtcatcg tcgggttcct cctcgtcgtg tccgccatgg
     4561 cgctgcgctc ggcgacgagc gtcgtcgagc aggcccgcgc cgagctcatc ggccagatca
     4621 cggcccgcca agaggctggc gacgcgctgg ccgagaaggt gagcgcactc gaggcagaag
     4681 tggcaggggc gcgatcggcg gccctcggtg catcggggca gtccggcgtc ttgagccaac
     4741 tggccttcct gcagtccgtg acgggagtcg tcgcggtcac gggtcccggg ctcaccgtga
     4801 ccctcgacga ctcggccgat gcggtcagcg gcgcggatgc cgcgcgcggg tccagcgggt
     4861 tcgaagcggg gcgggtgagc gccgccgacc tgcagatcgt cagcaacggg ttgtgggcgg
     4921 ctggcgcgga agccatctcg atcaacgggc aacgcctgac cacgcgctca gcgatccggt
     4981 tcgccggaca ggccatcctt gtcgacttcc ggcccctggt gcggccctac gtcgtgacgg
     5041 cgatcggcga tgccaaggcc ctgcagagtt cgttcgggtc ctcgccgacc ggtagttacc
     5101 tgaagtcgct cgaggacaac tacggcatcc ccgtggctat ctccgggagc gcttccttga
     5161 cctgccccgc gggcgtgccc agcgcgctgg tgtccgcgac ggcgcggccg accggcatac
     5221 cctcaaccac ggcggccccc acgtcgactg ccacgtccac gcgatccacg tcgacgacca
     5281 cgtccacaac caagacgtcc acgtccaaga catccacagc caagacgtcg acaaccaaga
     5341 cagccacgtc cccgatcacc cgggtgacca cgtcctcacc gagcacgacg gagatacggc
     5401 catgattcct gccatcggcc tgttcgtagg ggtcgtcctc ggcctgttgc tgcacccgag
     5461 cgtgccgctg tggctgcagc cgtatctccc gatcgccgtc atcgccgcgc tcgacgcggt
     5521 gttcggcggt gtccgcgccg tcctcgacgg gatcttcgtc gacaaggtct tcgtcgtgtc
     5581 gttcctgtcc aacgtgcttg tcgccgcctt catcgtcttc ctcggcgacc agttgggcgt
     5641 cggctcgcaa ctgtccacgg gcgtcgtcgt cgtccttggc atccggatct tctcgaacgt
     5701 ggcagccatc cgccgccacc tgttcaaggc ctgacgatgg ccgtccgccg accccgtcgt
     5761 cgccccggat cgaaggccgg agtggtcact cgcgagccgg aggagccgcg cgaggtcacc
     5821 tggcgccggt tgcgccggat gttcggtgcc cgcgcgacca gagccaacgt cctggccacc
     5881 gtcctcactc tggcgctggg tttcgccatc gccacccaga tccaacagaa ccaggaaggt
     5941 ggcctggcca ccctgcgcca ggacgagttg gtgcgcgtgc tcgacgacgt cacgcaacgc
     6001 tcgtcgcgac tcgacgccca ggtccgcgag ttggaggtgc agcgcaacgc cctgcaatcg
     6061 ggcgccgacc gggccaaggc ggccaccgag caggcgcagg agcggctgga cagcctcgcc
     6121 atcctcgcgg gcaccgtcaa ggcgacgggg cccggcatcc gggtgaccat cgccgactcc
     6181 tccggagccg tgcgtgcctc ggcgctgctc gacgtccttc aagagctgcg cgacgcgggt
     6241 gccgaggtcg ttcagttcgg cacggtgcgg atcgtcgccg ggtcctactt caccgacgcg
     6301 ggaacctcga tcacggccga cgggcagtcg ttgtcgcgtc cctacgtcat cctcgccatc
     6361 ggcgaccccc agactctcgc ctcggcgatg aacatccccg gcggcatcgt ggactcgatg
     6421 aaacgcctcg gcgccagcgc ctcggtcgac caactgagca ccgtcaccat cgacgcgttg
     6481 cacacgccga agacccctcg ttacgctcag ccggtgcccg aacccacggt cgccccgacc
     6541 gggggctaag gtcggctccg cagcgcctgc gacaccctga catcccgaca ccccgatatc
     6601 ccggccgaga acgtccgaac cacgccgcca cccgaaggag agccatgagc gagttgtcct
     6661 accccgagga cctgcgctac accagcgatc acgagtgggt cgccgtggcg tccgacggca
     6721 cggctcgggt gggcatcacc tccttcgccc agaacgccct cggcgacgtg gtttacgtct
     6781 ccctgccggc ggtgggtgcc acggtgagcg ccggcgatgc ctgcggtgag gtcgaatcga
     6841 ccaaatcggt cagcgatgtc tattcccccc ttgacggagt cgtcacgtcg gtcaacgtca
     6901 cgctcgacac cgcgcccgaa ttggtcaaca gcgaccctta tggcgacggc tggctgttcg
     6961 aaatgcgtcc gagcgacccg gaggcggtcg cacacctgct ggacgccgcg gcataccagg
     7021 cagtgctggg atagtccccg tatctgccgc gacagaaacg tcgcgggcgt atgattcgat
     7081 cccgggaatg cccgggacgg gacgcctggc cgggggtcgg cgccatgacg tattcttccc
     7141 gaacgatcac cgaatggccc gcggacctcg gtgacgtcaa acgggcatag acaccaggca
     7201 agcccgtgtg cggaaacgga gaagcaatgt cggacgagca cgagcgcccc gagaacgacg
     7261 agacgggcgc cccggagggt caggaagacc tgactcgacg ctcgcttccg tcgcgtcccg
     7321 gcggccccac gacgatgcgg ttccgcggcc tgggtgagat ccccgaggcc ttcgaccccg
     7381 acggggggct ctctgccgag gatgaggcga ccatcggtgc gttgcgacac ggaacggcct
     7441 tgctcgtcgt tgtccggggg cccaacaacg gggctcgttt ccttctcgac gacgccgagg
     7501 tgacctcggg gcggcacccc gagagcgaca tcttcctcga cgacgtcacc gtctcgcggc
     7561 gtcacgcggt cttcgtccgg gatggcaatg ggtatgccgt gaaggacgtt ggttcgctta
     7621 acggcaccta tgtcaaccgc gcctcagtgg acgcggccgt cctgcgcaac ggtgacgaag
     7681 tgcagatcgg caagtaccgc ttcctctact actcccgctg acggggccgc tgtggctgcc
     7741 cgtcgccgtg ggctctcgat cggcctggtc ctcgaacgac ttcagggtga gttccccgac
     7801 ctgacgatct cgaagcttcg ctttctcgag accgaaggtc tcgtcgcgcc cgagcgcgcg
     7861 ccgtcgggct atcgcctcta caccgaggag gacgtccgcc gactgcgcta cgtcctcacc
     7921 gcccagcggg accggttctg gccgctgaag gtcatccgcg acgccctcga cgccatggac
     7981 cgcggtctca ccccggctac ggacacgtcg ggggagcggc ccgccgtgcc gaccgcggcc
     8041 gccgaccccg acgtccctgc tgccgccacg ctggccgcac ccccgagttt gcgcctcacc
     8101 gaggccgagt tgatgggggc cgccgcgctc gacgaggcga tgttccaagc cttgcggggc
     8161 ttccacctgc tgcgccctga tgccacgggc cactatgacg acgccgccct cggggtggcc
     8221 cgcgccgccg ccgcgctggc ggcatacggc gtcgaggccc gccacctgcg gccgttccgg
     8281 acggcggccg agcgggaggt cgggctggtc cagcagatcg tcgtcccggc cctcgcctca
     8341 gccggcggcc gggcctcggg cgcggacccg accgccgagg tcctgcacca ctgtctggcc
     8401 ctgcacgcgg ccttggtgcg ggccgagttg cggcgcggat agcccgaggc gggccaccgg
     8461 ggtagcgtgg gccgggtgat cgaggtcgac gtcttgggcg ttcgcgtgga gatgcccacc
     8521 aaccagccca tcgtcctgtt gcgcgaacgc gacggggagc gctatctgcc catctggatc
     8581 ggcgccgccg aggcaacagc catcgcctat gcccagcagg gcgtcgtgcc gccgcgtcca
     8641 ctgacgcacg acctgctcaa ggacaccctg gaggcgctgg ggcacgccct cgtgcgggtg
     8701 cgcatcgatg aactgcgcga caaggtgttc tacgcgacgc tcgtggtcga cggcaccgag
     8761 atctcggctc ggccctcaga tgggatcgct ctcgccctgc gtacggccag cccgatcctc
     8821 gtccacgagg acgtctttac ggccgcgggc atcacgatgg ctcccgaaga ggacgacgag
     8881 gtcgagcgct tccgcgagtt cctcgaccag gtgtccccgg aagacttcga ggctccggac
     8941 ggcccgggag ggctgggtgg gtccgaaggg gagcgcccac cgtccccgga gcagtaactg
     9001 tcaacctgta cctgagagtt atgttacgca agtgatatct gcgacacgcg tgacaaaagg
     9061 tgcagtgatg attgaccctg aggccctcgg gccctaccgt caagggaaga agagcacggg
     9121 tgtaactacg cgcctggcgt tcggaccacc aggaggacac ccgtgagcgc gattgccccg
     9181 cccagccgcc ccgctggcgt tggtccggcg caagggctgc tcttcgacga cgatctgccg
     9241 gagctgcgcg aggacgaggg ctaccgcggc ccgaccgcat gccgcgccgc cgggatcacc
     9301 taccggcagt tggactactg ggcccgcact ggcctcgtgg agccgtccgt gcgcagcgcc
     9361 accggctcag gaacccagcg cctctacggt ttccgtgaca tcctcgtgct caaggtcgtc
     9421 aagcgactgc tcgacaccgg cgtgtccctt cagcagatcc gtcaggccat cagccacctg
     9481 cgcgagcgcg gtgtcgaaga cctggcccgc atcacgctga tgagtgacgg cgcctccgtc
     9541 tatgaatgca cctccgccga cgaggtcatc gacctccttc aaggtgggca gggagtcttc
     9601 gggatcgccg tgggtcgggt gtggcgcgag gtcgaaggat ccttgtctgc gctgcccagc
     9661 gagcgtgccg aagacgacga ggcgagcgcg cccgtgtcgg ccggcgatga gctgtcggcc
     9721 cgtcgcgcct cccggatgat ggcctgaccc caccgcgagc cggtatgccg cgcgagaggt
     9781 cgcgcgggcg gcataccccc gcgtggacgc gccgcaactg acgcggcacc cgcccgttcg
     9841 gcggctacac tcagtgccgc tgatcactcc acgtgggaga gtccatccac gccgggcctg
     9901 cgggccttgg ccaaggtggc gccgaagggg caactacccc ggaacctctc aggcaaccgg
     9961 accacgtgga caggcgactc tggaggggcc acggcggctc gacagagggg gccttgttgg
    10021 tcgtcctcga cgttgcccgg aggctcgtat gccgcagcac cctgccaccc cctcgaccgc
    10081 cctgcctgcc ttcgtcggga ggcacatcgg tccgggggag gccgacgtcg cgcacatgtt
    10141 ggcggtcgtc gggcagccct cgctcgacgc cctcgtcgac tccgccctgc cgcacctcat
    10201 caggagcgac gcgccgctgg cgctggccgc cgctgactcc gaggccgcgg tcatcgccga
    10261 gttgcgtgcg ctggccgggc gcaaccgcgt gctgcgatcg atgatcgggc tgggctacta
    10321 cggcaccatc agcccgccgg tcgtcacgcg caagatcctc gagaacccgg cgtggtacac
    10381 ggcctacacc ccgtatcagc cggagatctc ccaaggccgg ctcgaggcgc tgctcaactt
    10441 ccagacggtg gtgtcggacc tgaccgggct gccgatcgcc ggtgcgtcgc tgctggacga
    10501 ggccacggcg gccgccgagg cgatgaccct catgcgccgc tccaccaggc tgcccgacga
    10561 cgcggtgctg gtcgtcgaca cgcacacctt cccccagacg ctcgccgtcg tgcagacccg
    10621 ggcccggcca ctgggcatcg gcgtcgtcgt cgctgacctc tccacggtga cgaccggtga
    10681 acagttgcgc gccgctgccg gcggggtgcc ggttttcggc gtgctcgtgc agtaccccgg
    10741 ggccgacggt caggtgcggt cgttgagtgc tctcgctgcc gccgcgcacg agatcggcgc
    10801 gctgctcacc gctgcgaccg acctgctggc cctgacgatc cttgccgcgc cgggggagtg
    10861 gggagcagac atcgccgtcg gctcggccca gcgcttcggc gtgcccatgg gcttcggcgg
    10921 tccgcacgcc ggcttcatgt cggtgcgcgc cggcctggaa cgctcgatgc ccggccggct
    10981 ggtcggggtg tcggtcgacg cgcacggcgc gcccgcctac cgcctggccc tgcagacccg
    11041 cgagcagcac atccgccgcg agaaggccac ctcgaacatc tgcaccgcgc aggtcctgct
    11101 cgcggtcatc gccagcatgt atgccgtgtg gcacggcccc gagggcctgg ccgccatcgc
    11161 ccggcgggtc aacgcccacg cccggaccct cgcccgcggg ctgagcgccg gtgacatcga
    11221 ggtgctgacc tccgccccct tcgacaccat cagggttcgg gtgccgggtc gggcccacaa
    11281 actgcagcgc gaggccgccg cgcgcgggct gaacatctgg gtggtcgacg cggacaccct
    11341 gtcgctcagc tgcgacgaga cgacaacccc agaagacctc gacacggtgt tcgacgcgtt
    11401 cgggatcgcc gctcccggcg ctgctcccga gggttctgac ggtcctgagg gtgctgacgg
    11461 cgccgacggc gtcgggtggg acgcgagcct gagccggagc acggcatacc tcacgcaccc
    11521 ggtgttccac acccaccgca gcgagacgca gatgttgcgc tacctgcgcc ggctgtccga
    11581 gcgcgactac gcgctggacc gcgggatgat cccgctgggc tcgtgcacga tgaagctcaa
    11641 cgccaccacg gagatggagg cgatcacctg gccggagttc gccgacctgc accccttcgc
    11701 gcccaccaac cagaccgagg gcatccgggc actcatcggc caactcggtg actggctgac
    11761 cgagatcacc ggttatgacg cggtgtcgct gcagcccaac gccggttccc agggtgagtt
    11821 ggccggtctg ctggccatcc acgcctacca cgaggcccgc ggcgagggtc accggcgggt
    11881 ctgcctcatc cccgccagcg ctcacggcac caacgccgcc tcggcggtca tggccggcct
    11941 caaggtcgtc gtcgtcaaga ccgcggccgg gggtgacatc gacatggacg acctgcgcgc
    12001 caaggtcgag gcccatggcc ccgagctggc ggccatcatg gtgacctacc cgtccacgca
    12061 cggggtgttc gaggagacca tcaccgacct gtgtgccctg gtccacgacg cgggcggtca
    12121 ggtctacgtc gacggggcca acctcaacgc cctcgtcggc ctggcccagc cggggcggtt
    12181 cggcgcggac gtgtcacacc tcaacctgca caagaccttc tgcatcccgc acggcggcgg
    12241 cgggccgggc gtcggcccgg ttgcggtgcg ctcgcacctc gcgccctacc tccccaacca
    12301 cccgctggcc cccgaatgcg gaccctcgac cggcgtcggc ccggtgtcgg cggcccccta
    12361 cggctcggcg agcgtgctcc cgatctcgtg ggcctacatc cggctcatgg gcggcgccgg
    12421 gctgactcgc gccacccagg tggccatcct caacgccaac tacatcgcgg cccgcctgcg
    12481 cgagcacttc ccggtgttgt atgccggtgc gtccggtctc gtggctcacg agtgcatcct
    12541 cgaccttcgg ggcatcaccg cggagacggg cgtgacggtc gacgacgttg ccaagcgcct
    12601 cgtcgactac ggcttccacg cccccacgat gtctttccct gtggccggga cgctcatggt
    12661 cgaaccgacc gagagcgagg acctgggcga gttggatcgg ttcatcgccg cgatgatctc
    12721 gatccgcgac gagatcaacg ccgtgcgtga cggggtcatc acggccgaag ccagcccgtt
    12781 gcgctcggct ccgcacacgg catacgcgct gacgggcgag tgggaccgcg cctacgaccg
    12841 cacgaccgcg gtcttcccgc ccggggtgga cccgaccgac aagtactggc cgccggtggc
    12901 gcgcatcgac ggtgcctacg gagaccggca cctggtctgc tcgtgcccgg cgccggaggc
    12961 gttcgaggac tgactgggcg cgtttctgtc ttgatagggt cctgccgata gcctgtgggc
    13021 aaacacaagg tgaacgccac acaacgctgc ggcacgactt gaggagttgc cgggtgggca
    13081 agaagcaccg ccgcgagcag aagcttgcca agcagttggc caaggagttg gccaaggacg
    13141 agcagcgcgt cgggtcaccg atccccgaaa gcctgcgtcc gcgtccggcg ctgtcctacg
    13201 agttggacga ggccaaggag agcgttgccc tgtccgcgtt gcggcgggtc acccctggcc
    13261 tcacctacct gctgacgcag tatccccgcg ggatgatccg ccgggacgct gtcgcgggcg
    13321 tggcggtcgc ggcatacctc atcccgcagg tgatggccta ctcggccatc gtcaacgtcc
    13381 cgcccgtcgc gagcctgtgg accgcgctcg ccgcgatcat cgcgtatgcc gtgctcggca
    13441 gctcgcggat cctctcgtca ggacctgaat cgacgatcgc gctgatgacc ggcgtggcca
    13501 tcgccccgct ggcagcgagc aaccctgacc gggctgtcgc gttgtcggcg gcgctcgccc
    13561 tgtgcgtcgc cgggtggtgt ctcatcgccc gggtgctccg agcggggatc atcgccgagt
    13621 tgttgtcgac cccgctgctc gtcggctacc tcgccggtgg tgcggtgctg atgatcgtcg
    13681 gccagctggg caaggtgaca gggacctccg tcgacggcga gagcatcgtc gagcaattgc
    13741 agtcgttctt ctccgtcgtc gggcggacgc agatgccgac gctcatcgtc ggcggcgcca
    13801 ccctggcgct catcctggtc atcatcacgt tccggcccaa gtggcccgcc ccgctcatcg
    13861 cggtcgtcct cgccacggtc gcgtcggtcc tgctgcacct ggaagcccag ggcgtgaagg
    13921 tcgtcgggac cgtgccgtcg gggctgccga tgccgcacct gccgatggtc acgggcgagg
    13981 aattcaaggc gatgatctgg gccgggctcg gcatcgccat cgtcggctac agcgacatca
    14041 tgctcatctc gcgcggcttc ccgctgaccc cgggcgaggg ggagaccaag gccgatgtgc
    14101 gcgccgaccc ccaacaggag ttggtcgcga tggcgggggt gcatgccttc gtcggcttct
    14161 ttagcggtta cccggtgtct gcgtcgggct cgcgcaccgc actggccgtg gcaggaggcg
    14221 cacgcagcca ggcgtattcg ctcgtggccg ggctgtgcgt tgtcgcggtg ttgttcttcg
    14281 ccggccccct catggcaggt ctgccgtcgg ccgcgctggg tgcggtcgtg ttctacgccg
    14341 ccgggaagct tgtgtcgatc cccgacttca aacggctgtg ggccttccgg cgtcgcgagt
    14401 tcttcctggc catcatcgcc atgctgggca ccgtcgtcat cggcatcctg cagggcgtgc
    14461 tcttcgcgat cgccctgagc ctgctggaga tgctccaccg cctcgccagg ccccacgagg
    14521 gcgtcctcgg gcgggttcct gggatcccgg ggatgcacga tgtcgccgac tatcccgatg
    14581 cccgcacgat tcctggatgc atcttctatc gctatgacgc gccgctgttc ttcgccaacg
    14641 tgggggacct gcgcgaacgg gtcgaccggg tcatctccgt ggagaaccag gcctacccgc
    14701 agactccggt gcgctggttc atcctcaacg tggaggccaa tgtcgaggtc gacatcaccg
    14761 ccgcggatgc cctgcgcgag ttggcccagg agttggccga ccgaggcatc gagcttggcc
    14821 tggctcgggt gaagttcgac ctctacgagc cgcttgaccg ggccggcgtc atcgatgtca
    14881 tcgggaagga catgctcttc gccaccctgc ccgtggccga agaggcctat ctcaagtggg
    14941 ctgtggccca agcgaactcc ggatcggtcg agcccgacct cgaagtggtg caggccgcgg
    15001 tcgattccgt gccggcacac agcctgctgc cgtgggatcg ccccgatgat gaggaggatg
    15061 acgatggccc gcggccgaac ctcgggcctg ccgaagcgag agtggccgtg ctcgatccca
    15121 aaaccgccgg cccggaccaa cccgccgacg gcgtcgccag gtgatcaggc ggccgggccg
    15181 gtgactcgcc ggaccgtcga gccccgtctg gtcttgtcga cctgcacctg agccgggatc
    15241 cggtcacgca aggccggcac gtggctgacg accccgaccg cgcgcccacc gtcctgcagt
    15301 tggtcaagca cctccatcac ctgttcgagg ctctcgccgt cgaggctgcc gaacccctcg
    15361 tcaatgaaca gcgtgtgcag gtcgatgccg ccgctctcct cgcggaccgc gtcggccaac
    15421 ccgagggcca gagccaggga ggccatgaac gactcgccgc cggacaaggt ggcggtgtcg
    15481 cggacctgtc cggtccactc gtcctgaacg accaggccca ggccactgcg ccgtcccccg
    15541 gaagcgagcc ggtcgctgtg ggccaggtgg aatcgcccgt cgcccatccg ctggagtcgc
    15601 tcgttggcca actcgacgac ccgctccaac cgcccggcca gcacgaaggc cgagagcttc
    15661 atccgcagcg tgttgtccga gccgagcccc gacacgacat cggcgagggc ggcggtcgag
    15721 gttgccaccc ccgttgcggc tgtcgcggca gcggcgctgg cggccacctg ccgcgacagc
    15781 gcgtcgatgt cgcgcgcggc ctgctcggcg agggcatgct cgcgtgccgc ggcggtgcgt
    15841 tggcctcggg cggcgttcat catggctgcc aagaccgcaa ggtcggggag cggggctccc
    15901 agcgcacgct cgacctcggg atcggcgacg gtggcccgtg ccgcggccgc ggcggcaacc
    15961 ttcgccgcga tgccctcccg aatttcaacc agcgtggccg cgggaacggc cgccgcgcag
    16021 gcctgcacga catcggcgaa gccctcggcg cggcatacgg aggaggttgc ctcgtgcgcc
    16081 tcggtatgcc gccgctgtgc ctcgtcggac gccgaacgcg cggccgcggt gtccgccaac
    16141 gcagcgacga cgcggcggtg gcgcgctggg acgtcggccg tgggcacctg cgggtcggcg
    16201 cacggacacc cctcggtatg ccgtgccacc agctcgcgga ccacggccgc atcgtgctcg
    16261 gccgcctcgg ccagctcgcc ttcgagagcc tgggcggcgg cgagctgccc gagggctgcc
    16321 cgtcgggact cggcgagcgt ctcgagggcc tggcggcgtt gcgcgagctc ggcttgcacc
    16381 tgggggagct cggccactgc ggaggcagcg acggcgacgg ctcggccgat gacgtctgcg
    16441 gcgtcgcgaa ggatcgccag atcgcgctcg tcgccgccga gctgttccac gaggaggtca
    16501 tggtgagctc gggcggcggc cagatcggtc tcgatgcggc ggacccgatc gtccaggggg
    16561 cgccaggcct cctcggccgc gtcgacatcg gcagcagtga cagcatcgac cgcggtcgcc
    16621 ggggctgggt gctcggtgcc tccgcagacc gggcattcgt cgcccgggtg caggccggcc
    16681 gcgagctcgg cggccatccc gtccaggcgc cgctggcgaa ggtcgagcaa cctcccggcc
    16741 tgggcgaggg cgacccgggt ggcggccagg ccggccgact caaggctcgc gatggtgatc
    16801 gccgtgcggt cgagatcgtc gcggagggtg accagtcgcc cggcctggga cgctgacgcc
    16861 tcggcctgcc cggaaccggc ggcgatggtc agcagcgcgt cagcccggac ctgcagctga
    16921 gcgacctgct ccactaacgc cgcctcggct gcgtctatgg tcgccagcgc gcgctgcgcg
    16981 acctcgcgct ggacgaacac gctcgcctgc cgttgcgtcc gcgtggcctc cgcagcgccg
    17041 cgctgggcca acacatccag agaggcgtcg agaccgcggg ctcgggacag cgcctcctgg
    17101 ggcgagtcac ccacgtcgac cgaccaccgg gcgagggcgg tacagctcgc ggcataccgg
    17161 gtgtgggccg agtctcgctc ggcctcggcg gcggcgagcg cacgcaggtc gccagccacc
    17221 cgaccggccc gctcgcccaa ctccaggcgc cgggccgccg ccgcgcgagc atcggagccc
    17281 gccgcgaact ctgcgagcac ccggcgtgcg gcctcgccgc gagcagcggc ccgcgccgtg
    17341 agagccgctg ccgcatgggc gctctcggcc gcactggcag cctgctcggc cgtcgccgcg
    17401 gccgtgagag ccgccagcgc atgggcgcca acccgggtcg acgcctcggc agcaatctcg
    17461 gccagccggc cagtttcggt cgcctcggcc cacgtgggca gcccgtcgat gacctcggcc
    17521 ggaacccggg ccagcacgtc gtcgacccgc agcagatcgg cggagagtgt ggcgcgcgcc
    17581 tcggccgctg tggcggcggt gaggcggcgg cggtcggtca gccaggcttc aacaccggtg
    17641 tagcggctga cgtcgaagag gcgctccagc agcacccggc gttcttccgg ggtcgcccgc
    17701 aggaaggccg caaagtcacc ctgggggagg aggaccacct tggcgaactg gtccaggccc
    17761 atgcccaacg cttggccgag gctgtcaccc acgtcatcgg ctcgggtggc cagcgtcgtc
    17821 cacaccccgt cgcgggacag ctcgtcgagg cgcacctgac cgcggaccgg ggtgaggccg
    17881 ttgccacgct tcttgggacg gtgatggtcc ggagaccggg tcacccggag cctgcggttg
    17941 ccgacggtga gctcgagcgt cacctcgggg acgtcatcgg ctcccgcgtg atcgctgcgc
    18001 aacgtctcac cggccgggcg cagacctggc acccggccat agagcgcgaa acagacggca
    18061 tccaggaggc tggtcttgcc gctcccggtg gccccgtgga tgaggaagat gccggcgtca
    18121 gcgagctcat cgaaatcgac ctcgatcagc tcggcgaacg gaccgaacgc gcgcgcctgc
    18181 agccggtgca gcctcatgcc acaccccggg actggtcggc cacgccttcg tcgtcggcct
    18241 cggcccgggc tgccctgctg gcgtcgaggg cctcggccag ggtggctcgc tcgtcgtcgg
    18301 aggcggccgc tcccgcgcgc acgtgggcga ggaagtcgca gcagacgtcg agctcggcgc
    18361 gggcggaggg gacgcgagcg gcatacctgc tcgtggcgtg aagctccccg ccacgcgggg
    18421 caaactcgag gtgcagcagg tcagggaaac gacgggtcag ttgctcgtat gccgcgagcg
    18481 gccggaccgg gtcggtgagg acggcccggc accaggcacc gcgagcccac tccagggagg
    18541 ggttggtcaa caggtcggcg aggtcgccgc gcaggacggc caaccgccgg ggcaccgggg
    18601 tcgggatcgc ttcgacggac acgccggtgg ccgtgaggtc gagcagggag accgacttga
    18661 tctggtcggc ctcaccgaag gacatcgcca acggcgagcc gctgtaggcg acgtcgtcac
    18721 ggacccactg ggggcggtgc aggtgaccca gagcggcata cgtcacgccg ttgaagacgt
    18781 cggccgggac gacactcacg ccgccgacgg cgatctcgcg ctcagactcc gtcgtcgtgc
    18841 caccggcgac gaaggcgtgg gccatgacga tcgagggcag gtcgtggctc gtcaggtcga
    18901 cacggacccg atccatcgcg gcccgcagca cggcggcgtg cgaccgctcg ccgaccccta
    18961 gcgctggacc gaccaccgca ggctcaagat agggaagcgg atagacggcc acgtcgccga
    19021 ggacgaccgg ggtgccgacg ctgctcagcg acgcaccgat gtgcaccccg gctcgggcca
    19081 gcaggtcgga ggcgaaaccc aaccgaatgg ccgaatcatg gttaccgcta gtgagaacga
    19141 cctgggcgcc ggcgtcgact aaccgggtca gcgcgtcggc gagcacgctc acggtgtcag
    19201 gggaaggcag cgcccggtcg tagacatcgc ccgacacgag gacggcctcg accccctccg
    19261 tgcggaccag atcgacgagg aattcgacga actgcacctg ggcaccaagc agccccacct
    19321 ggtggaacga gcgccccagg tgccagtcgg aggtgtgaac gatgcgcacc cggccaggct
    19381 atgaccggcc accgacaggg cccgggaggt gggtcggcgt gcctcccgtg gacccaccat
    19441 cgcgcgcgaa ccgggcagag cggcatactc tccggccatg ggccaggaca tcgagtcgat
    19501 cacatacacg cgcgagcagc gccaacggtt ccggcagaag gtgcacctgt gcctggacgt
    19561 gttcgagcgg atgctggcct acagccagtt cgacttcgag cggccgatga ccgggatgga
    19621 gatcgaactc aacctgacgg gaagtgactt ccgccccaag atggacaacg ctctcgtcct
    19681 cgagcggatc gccgacccgg agtatcagac cgagttggcc cgctacaaca tcgagttcaa
    19741 cgtcaagccg cgcgagttgg gcggcgactc gatgctgtcg ctcgagggcg acctgcgcgc
    19801 atcgctcaat cgggcccatg agaccagcgc ggaggtgggg agcaagatcg tcatggtcgg
    19861 catcctcccc accgtgaccc tcgacgactt cgcgggcgac tggatgagcg ccaacgcccg
    19921 ctaccacgcc ctcaacgacg ccatgctcgg cgcccgtggc gaggatgttc acctggacat
    19981 cgaggggcct accggcgagc acctggagcg ctatttcgac tccctggcgc ccgaggcggc
    20041 ctgcacgtcg atgcagttgc acctgcaggt ggcgcccctg tcgtttgccg accactggaa
    20101 cgccgcccag gccctcgctg gcgcccagtt ggctcttgcc gccaactccc cgttcttctt
    20161 gggcaaacgg ctgtgggccg aatcccggat cccggtgttc agccaggccg tcgacacccg
    20221 ttcgccggag ttgaagaacc agggggtgcg gccgcgcgtc tggttcgggg agcggtggat
    20281 cacgtcgatc ttcgacctgt tcgaggagaa cgtgcggtac ttcccggcgc tcctgcccga
    20341 gaccaccgac gaagacccgt cggccgtgct cgatgcggga ggggtgccgg ccctggccga
    20401 gatgcgcctg cacaacggca ccgtctaccg gtggaaccgc ccgatctacg acatcggcgg
    20461 cggccaccca cacgtgcgcg tcgagaaccg ggtgctgccc gccgggccga ccatcgtcga
    20521 caccctcgcc aacgccgcgt tctactacgg cgccctgcgg atgctcgccc aggaggaccg
    20581 cccagtgtgg tcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttctac
    20701 tacggcgccc tgcggatgct cgcccaggag gaccgcccag tgtggtcgcg catgagcttc
    20761 gccgcggccg aggacaactt cctcgccggg gccagggacg gcctggaggc caagctctac
    20821 tggccgggcc gaggtgacct gccggccacc gagctcatct tgcgacacct gttgcccctg
    20881 gccaacgagg gcctgcggga ctggggtgtc tcgacggagg cgcgggaccg ctacctgtcg
    20941 gtcatcgagg gccgttgcct cacgggggtc aacggcgcga cctggcagac ggcgaccgtc
    21001 gcggcgctgg aggagcgcgg catacctcgg gaggaagccc ttcgccagat ggtggcgacc
    21061 tactctgagc acatgcatgc caacaccccc gtccacacct ggccgatgcc gtgacccgcg
    21121 cgttccgcct gctcaacgtc ttcgcgctcg ctgacgaccc cttctcgggc aacccgctgg
    21181 ccgtcatcga ggacgcgtcc gggctcgacg gcgccacgat gctcgcgatc gccaggcagt
    21241 tcaacctgtc cgagacgacc ttcgtcacgg ccggcggcga cacgagcggc gccggaccgg
    21301 ccgacgcaac agtgcggatc ttcacgcccg gctacgagat gccgttcgcc gggcacccga
    21361 ccctcggcac ggcctgcgtc gtcggggagc gcctgggcaa gaaccacgtc gtcctgcgtg
    21421 tccctgcagg ggacatcccc gtcgacgggc tgagcgttcc acacagcggc gaccagacct
    21481 ggaccctcac cgccaaaccg gccacgatca ccgaaggggc ggtcgctcgg agcgacctcg
    21541 cgtccgccgt cgggctcaac gccgacgacc tcgtcgacga gggctggtgg gtcgacgccg
    21601 gcgtggatca gttcctcacc gaggtgacgt cggtccaggc cgtccgacga gccgcggccg
    21661 acccggtggc ggcccggcgc ttcatcctct ctccgcgcgg cgagagcatg gccctggtgt
    21721 gggcatggac cagtcccgac acgatcgagg cgcggttctt cttcagccag gacgccgcga
    21781 tgctcgaaga cccggcgacc gggtccgcgg cggccaacct gggcagcctg ttggcccatc
    21841 ggggcttccg cggggcggtg acggtggacc agggtgctgc ggtggcgcgc ccgtcccggc
    21901 tgcacatcga ggtcaccgag gccggccgag tccgggtcgg cggattggtc cgcgagatcg
    21961 gccgcggcac cctcgcgctc tgatgccgac ctacgtcgcg ttcctgcgcg cggtgaacat
    22021 cggtcggcgc cagaccccga tggcccgcgt gcgggccacc ttggaagcga gcgggttcac
    22081 cgacgtccag acctacatcc agaccggcaa cgtcctgctc accacgaccg cgcgctcgcc
    22141 ggagaaggtc gcccagcggg tccgcgaggc cctctccggc gacttcggct tcgacatccc
    22201 cgtcgtggtc cgtcggccgc gtgatctggt cgatctcgtc gccgccgtcg acgccctgcc
    22261 cgatccgttc ccgcaagggc gcacgtatgc cgcgttcctc gaccaggagc cgagcgacgc
    22321 agcaaccgca gtgctcaacg actggtctgc gccgggggag tgggtccgcg tcgtgggacg
    22381 ccacgtcgtc ctgcggctgg gcattcccgc gaacgtcgtc accttgacca acgcgaagat
    22441 cgaacgtttc ggcgtggtcg cgacgacccg cgatctcacc gtcgtgcgcg ctctggcgac
    22501 ccgctggggc ggttgagccc gcgacgaacg acgtcgaaac acacgaccgc cgccgcggcc
    22561 gacacgttaa gagactccac gcctcccgcc atggggatgg acacccaggt gtcaaccaac
    22621 tcgctcacct cggctcccag cccggccgtc tccccgccca tcacgtatgc cgtgtcgccc
    22681 ggcaggtctg ctgcgaacag gtccgcaggc ccatcggcat ccagcccggc gatccggtag
    22741 cccgcgccca gcagcaaccg gacggcctcg accgccgtcg ccaccttgag gatgggggct
    22801 ttgaaggcga cgccggcgga cgccttgacg acgagcgggt cgatcgacgc gactccccgg
    22861 cgcggcacga cgatccccga gaagcccgcg gccgtggccg tccggaggat catcccgacg
    22921 ttggccgggg tggtgatgcc gtccaggacg atgatcgggc ccctcagccc gtcggccagg
    22981 gcgaggtcca gcggtctcat ccgcggggcg acgacgtcgg cgcacacgcc ctggtcctgc
    23041 ttgccgttgc cggcgatctt cttgacccgt tcggcgccga cgagttgcac cgggacgccg
    23101 cgggcgcgcg cggccgagcg gatctccgac acgtgtgggc cgtgggccga gtcggccatc
    23161 aggaccttgt cgacggtcag cgaggggtca ccgagcgcct ccagcacggg gttgcggccg
    23221 tagatcgtca caaaccggtc tttcggtgag atcggtgtcg aagcgttggg tccggtcacc
    23281 cgcctatcca accggttcac tcgtccgagt gagggctagc ggcacgtgta aacgcttacg
    23341 tatgctgtgc ctcgggctcc acgagcccgc aatcgcgtcc gtttctgacc gagtctgagg
    23401 gagccatgac cgaccgcgtc ccgcgtcgcc gtcttcggcg ggagggctcc tcccgatgac
    23461 ctccacggca cctgcggcgg tggtcgacgc cactgcgtcg atcctcgccg acctctcgcc
    23521 cgagcgccgt gagctcttcg agttgcggct gcaccgatgg tgggacgacc tcctcgacgc
    23581 gctggagacc ctctacggcg agcgggcctt cgacgtggcg cagcggctgg tcgcccatgc
    23641 ggccgcggca taccgggacc gcgatcccga gttgcaccga ctggacgaac gacgcatcct
    23701 cgcgcccgac tggctgcagg acccgcggat gttcggctac gccacctacg ccgaccgctt
    23761 cgcgggaaca ctggaagccg tcggcggcaa ggtcgactac ctcgaagagc tcggcgttac
    23821 ctacctgcac ctcatgccgc tgctgcgccc tcgcgagggg gacaacgacg gcgggtatgc
    23881 cgtggcctcc taccgcgaac tgcgccccga cctcgggacg atcgaggacc tcaaggcgct
    23941 gtcgaagctc cttcgcgaca agggcatcag cctggtcctc gacctcgtgc tcaaccacgt
    24001 ggccaaggaa catgactggg cccagcgggc ccgcgcgggg gagtcgcact accgcgacta
    24061 cttctacgtc tatcccgacc ggaccgagcc tgacgcctac gaagcgaccc tgcctgaggt
    24121 cttccccgac ttcgcccccg gcaacttcac ctgggacgac gcgatgggcg ggtgggtctg
    24181 gacgaccttc aactcttggc agtgggacgt caactgggcc aacccggatg tcttctgcga
    24241 atacgccgac atcatcttct tcctcgccaa cgccggcgtc gaggtcgtcc ggctggacgc
    24301 gatcgcgttc acgtggaagc ggctgggcac caacagccag aaccagcccg aggtgcacgc
    24361 cctcacccag gcgctgcgtg ccatcgcccg gatcgcttgt cccgcagtcg cattcaaggc
    24421 cgaggcgatc gtcggcccgc gcgacctcgt gcagtatctc ggccagggtg agcaccacgg
    24481 caaggtcagc gacttggcct accacaactc gctcatggtg caggcctggt cgatgttcgc
    24541 cacccaggac gtgcggctga cagcccatgc cctctcggcg ctgccgccgg tcccgacgac
    24601 cgcgacctgg atctcctacc tgcgttgcca cgacgacatc ggctgggcca tcgacgacgg
    24661 tgacgccgct gcggtcgggc tcaacggctt cgctcaccgc gccttcctct ccgactggtt
    24721 catcggtgcc tatccaggct cgtccgcccg cggcctcacc ttccaggcca acgaggccac
    24781 cggtgaccgg cggatcagtg gcaccgcggc ggcgttgacg gggcttgacg ccgcacggga
    24841 ggtcggcgac caggcagcgg tcgaccgcgc catctcgcga atcctgttgg ggcacgctct
    24901 gatcgccggc ttcggagggg tgccggtgat ctggagcggt gacgagttgg ggctccccaa
    24961 cgacccggtc tgggccggag agccgggcca cgaggacgac aaccggtggg tccaccgagc
    25021 ccggatgcac tgggaggtgg cggagcgacg gcatacccct gggacgcccg agcaccgcgt
    25081 gttcggcggg atcgcgcata tcgcgcacac ccgcgcccgg ttgccgcacc tgcacgcgtc
    25141 ggtgccgtcg agcgtcctcg gcgtctggga ctcgggtgtc ctcgtgttcg cgcggcgcca
    25201 cccggtgggc accatgctcg ccgcctacaa cgtcaccgat tcgtggcgcc ccgtgccggg
    25261 ctgggtcctg cggcaagagg gcctgtccag cgggacggag gccctctcgg gcgcgcacgt
    25321 acggatcgag gccgacgaca acgcctggat gcccccgtat gccgtgtggt ggctggtcga
    25381 ccacatcccg gcgcacgcat agggtcgggg tgtgcagcag agcgtctcgt tcaaggtcta
    25441 ttacgaagac accgacagcc tcggggtcgt ctactacgcc aactacttca agttcatgga
    25501 gcggggccgg tcggagttcc tgaccgctca gggccggtcg gtggccgact ggaacgccgc
    25561 gggcttcctg ttggtcgtgc actcggtgca ggcgaccttc aagcgacccg cagtgttggg
    25621 tgacaccgtc gacatcgtca cgtcgttcac gttgccgtcg gtctaccgcg gccggttcgc
    25681 ccaacgggtg gaacgggcag gggagttgct cgtcgacggg atcgtcgacg tcgtgtgcct
    25741 ggacgcggcc cagaacctca tcgagttccc gcagcagttg cgcgccctcg ccgattgagg
    25801 cccggctgta ctgcggcagc cggcccgtca gagcaagccg agttcctggg cctcttccca
    25861 gaggtcgtcg cgggcggccg ggtgtgcggc atgctcgatg agttgcaccg cttggctctt
    25921 ctggtcgcgc caggcgatct ccgccacacc ctgctcggtg atgacggcgg tgtgttggaa
    25981 ggacgtcacc ggtgcctcga tgagcggcac gatggtcgac acgtcggcct tggggtgcca
    26041 ggaacgcagc gccatgaggg cctgcccgcg cggcgcgtgc agggcaccga cggtgaagtc
    26101 gctctgcccg ccgaagcccg aatagacctt ggcgttgacg ctcgccgcgt tggcctggtc
    26161 gtagaggtcg acctgcagcg ccgtgttgat gcttgtcatc agaggctggg tggcgatgat
    26221 cgacggcgag ttggtggtct cggtgcgcag gacacgcagt cgctcgttgc ggtgcgccca
    26281 ggcgtagagc gcggccgacc ccatgagggc cgacgcggtg atggtgcggg ccgggtccaa
    26341 cgccccggcc tggtccagct ccaggacccc atcgccgatg acttcactcc agatccccag
    26401 gccccgcttg ccgcgcagcc cgtccagcgc tgcgtcgggg atctcgccga tgcccagttg
    26461 cagggtggca ccgtcagcga cgcgcctgga caccaggtcg ccgatgatcc gggcactgtc
    26521 cgcgcgcgac atcccgccgc cgctggccgt gccgtcggcc tctgacgtgg ccaggttgag
    26581 caccggctcg tcgacctcga cgatggcgtc gaagacgtcc agcggcagtt ccccgtcacc
    26641 gaacgtgtag ggcatctgcc ggttgacctg cgcgatgacg atcccgccgg cggccttgca
    26701 cgccgcgatg gctccgggca ggacgttgat ctcaagcccg agggacacct ttccggcgat
    26761 tggcgcggag gtgtgggcga tgacgacgtc gacgcgcagg cgccgctgat agaggaacgg
    26821 cacccctgag agccgcatcg ggaagtagac caaacggggg tggtgacgca tcccgacccc
    26881 gacgaacgcc gactcgggga tcaccccatc gcgctgcggt atgccgcgcg gcgcgttgag
    26941 caggtgcagc cggtattccg ccaggagggt gtcgaccttg ccgaggacgg tcagcggagt
    27001 gctgcagttg ccgctggtga cgacgcgcag gtcggtgcgc cggctgtcga tgacagtccc
    27061 gaggtggtcg atgtcgagcg tctgcacgca ctcactctcg caaactcggc gcgcgacgcc
    27121 aatgccgggc gagtcctcgc tccgcagcga ggaagccacc ggccatgagc aggcccagca
    27181 cgaggatccc ggcgacgttg cccagagccc gggcatagcc caggacggcc acatccacga
    27241 aggggtaggg ataagcccca acaaacgccc cgtgcagcag catgacgacg agccaggcga
    27301 tggggatggc gagcgagtgg acgacggtgc gccggctcac ccacccgcgc ggtccgacga
    27361 gcacccacac gagcaccgtc aacaccggag tcacttggtg cagaagggtg ttggagacgt
    27421 tctcccaccc ccgctggacc gagacgggag ccagcaccac ggcatacacc aggccggtga
    27481 tggtgatgag caggaccgag tcgaggcgag ccgcgcgcaa ggccgcgccc acccgggggc
    27541 ggccccaggc gagcagcgag gtcaccaccg cgacaacgat gttcgaccag attgtgaagt
    27601 aggagaacca gtcggccagc cgcgatccga ggccggccgc gtggtccgcg ccgtaacggg
    27661 tcgggaccgt caccgtgctc gggtagatgt tcgtcacggt gagggtcagc gagaccgcca
    27721 accccagcca ggcgaggact gcgttgaggg cgagccagcg acgcgcgccc gcagtgaggg
    27781 tgtggtccac gaggggcgag tatgccgtgt gctcgggtca ggcgtgcgcg tttggcgagc
    27841 gtcggagccg gaccatgacc tcgtggtggt cggtctgggg gaacatgtcg aacagtcgcg
    27901 cctggtgggg gatgagcgag ggcatcctcg cgaggtcccg ggcgagagtg gtgacgttgc
    27961 aactcgaata gacgacgtcc cgcaccgtgc tcgactccag ccaactggtg agcgtctcgt
    28021 ccaggccgcg acgtgggggg ttgacgacaa cgaggtccgg ggccgggtga gcgatcgcgt
    28081 atggcgtcgc gtcaccgacg acgaagtcga ccggcccgca gtcggggcgt tgcgcgagca
    28141 gacccgctgc gctgcgttgg gcggcggcga tcgcatcggc agacacctcg acaccgatga
    28201 cgtggcggcc tgctgctgcg gcctgaagcg cgaatccgcc gacgccgcaa tagaggtcga
    28261 ggacggaggc cggttgggcc gcctcgatcc aggcctgggc ctggcggtag agcccggcgg
    28321 cgaccgccgt gttggtctgg aagaagctcc gcgggccgag gtggagctcg gcgtcggcga
    28381 ccggcatcga caactcggac tgcttagtga ggacgagttc ggtgtcgcct tcgaggaccg
    28441 ccttgtgttg cgggtggaga ttgacggtga cgacctgcag gtgggggagc gcggcttgta
    28501 gccgggggag tgcggaacgg gcctggggga gttgagaatt ggagcgcagg acgatgcgca
    28561 gcatgagggc gccggcgggt gacgcggtga ccaggaggta cttcagctcg ccgcgccgct
    28621 gggggacgtc gtagggggtg agttgggcgt cgatgacgaa gtcggccagg tgggggagcg
    28681 ccgcgtgtag ggccgggtcg tagagcccgc agtcgcgtag gtcgacgcct cgaccgtggc
    28741 cgtcgaggat tcccagggtc ggcgctgagg cggtgccacc gacgacgagc ttggctttgt
    28801 tgcggaagcc gtgctcggcg ctggcgaagg gctcgagcca atccagttgc ggcgcaatca
    28861 aagacagcgc gtcacggcac ctcgcgtcct tggccgcgag ttgctgggag tatgccgtcc
    28921 ccatgagcgt gcacgagcgg catacccccg cgtcgtagta gtcgcactgc acccgcctga
    28981 gggtagtcgc cttccccgaa tccctcacac tcgccggcga tctcgagccc gcctcgcccc
    29041 ggcttccccg aattcctcac actcgccgac gatctcatgc gtacctcgcc ccggtttc
//
LOCUS       DAORPW010000013        27942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_250_length_27942_cov_763.773084, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000013 DAORPW010000000
VERSION     DAORPW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 27942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27942
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_250_length_27942_cov_763.773084"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PLB86_02300"
     CDS             complement(<1..548)
                     /locus_tag="PLB86_02300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC63997.1"
                     /translation="MNLRESIASQPMRAFQKRIIGICIALAFVDGFEVLVAAFTSTAV
                     AKAFALPNAADIGYFLSAGTFGMGLGAILISPLADRIGRRKHILMCLALICVGMAASA
                     LAPTSSFPFLLAARFFAGLWIGALIPSLNILVSEYSSDAKRGTAMGVYGVGLPAGAAT
                     GGFITTFLLNAWGWSAPFWFGFA"
     gene            complement(612..2144)
                     /locus_tag="PLB86_02305"
     CDS             complement(612..2144)
                     /locus_tag="PLB86_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRC63998.1"
                     /translation="MTFNLSSILRDSAQRDQEHPAIVTGSERISYGELDRRSDAVAAG
                     LMAAGLEPGGAVMLQLPNVPEFVISLHGILKAGGVVIPTNTLYKAGELSYVLENASAK
                     HVITVDASAGEAAEAAAQAGGATLYVLGTVPESLPDGVVARPFSELADHPVPDPKPFI
                     QRDPGDTAVLLYTSGTTGKPKGVQLTHFQLYMNAGAHIGAFEMGPTSKIIAVMPLFHA
                     LGLSGILNATVRAGGTVLLLPKFDTEKVLQTIQAEGATHIHGVPTMYHSLLNHPTLAN
                     FDTSTLAFCGSAGAAIAAEIIDQVEKTFGVQILEMYGLTESGPVATINMPRDRKPYSI
                     GKAIPGVDIEIWDEQKRRLPRGKLSIGEIMIRGHNTMSGYLNNPEATAAAFTNGWLHT
                     GDLAWMDEEGFLFFADRKKELIIRGGYNVYPREVEEVLYTNPKISEAAVVGVPDERLG
                     EEIKAYLTLKPGEAGTPEEFIDYVKERLAAYKYPRIVEVIAEMPKSATGKILKKELAH
                     RR"
     gene            complement(2239..3450)
                     /locus_tag="PLB86_02310"
     CDS             complement(2239..3450)
                     /locus_tag="PLB86_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC63999.1"
                     /translation="MGEGTTESAREIVVVGGGIGGLTTALALARDGRTVRVLERAERF
                     AEVGAGLQLAPNATGILRRLGVLDAVLDVGVAPARIVVADARTGEGLVSADLGDRFLR
                     TFGSPYLVVHRGDLLDVLLQAARSAGVVLENGKAVVRTETVGGETRVVCSDGSEYAAR
                     AVIAADGLRSHLREAIHGASEPIASGYVAYRGALPIEQVERPHDLDTVISWVGPGLHL
                     VQYPIRRGDLYNQVAVFKSPSFATGNPDWGGPDELDAAFSGMCPAVRVALPALQRNFR
                     WPTFDREPVDTWVDGRTALLGDAAHAMLQYLAQGACQAIEDAATLAQQFAAHDGATAD
                     DGGVDRALAAYQRIRAPRTARVQRSARVWGDIWHINGTGALLRNELFRGITDDDYSYA
                     DWLWGWEPPTN"
     gene            3617..5047
                     /locus_tag="PLB86_02315"
     CDS             3617..5047
                     /locus_tag="PLB86_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRC64000.1"
                     /translation="MKATQLTAFDASFLALDTNISVGHVTLLSLLDREVSPDELFHQI
                     EAHLHLAPMLRRKLRAPSLIVGRPWWVDDPDFDLDDHLTFTDLGGHADEAAMCAEAVR
                     FSRLHLDRSRPLWELLLIGGMADGTGALVSKIHHCAVDGVGKRDLLSLLFGTESSLDP
                     HRRWRPDGGPGQLSRITHRVTEATERTGAALRLEFQAVTAAPAAVLKATRDTVTRLAD
                     GLERFVEGAESVEAEPAWARVGSPPATPFNAAITAERAWAWLPMPVAASSALRRATGS
                     TLNDVLMAATAGALRDWLAENDALTTDPLIAMVPMSIRGQGHSVGSNQIMLTLCPLPT
                     HLDTPRERLAFAHSAMDHAKATPTYPSTLMSDLAAVAGPTVTQLLTQAAAMVRLADRV
                     QLPFNLMVSNVPAPAREFIVGRGARVIGSFPFPPLSDGMGLSVCAQGYPGALDIGIGS
                     CSDLLPDPWRVRDLLEQAHEDLVASA"
     assembly_gap    5075..5174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5239..5826)
                     /locus_tag="PLB86_02320"
     CDS             complement(5239..5826)
                     /locus_tag="PLB86_02320"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC64001.1"
                     /translation="MSAPQAPQALVRRVDERSVDVVCDLWAQQREERAPTELGLRRVT
                     ADVVRAALLRPETVAFAAYLNGAAVGYVVLSDCSLNPFSDAACVSVDQIYVAKDARKK
                     GVARALMTAVATFADRHGADQIACSVPSAVRDSNRFFARLGFTPHTIRRVTLTAALQR
                     RLVGDTAESRFALDQVLARRRVARIRAAQGRFAAH"
     gene            6028..6885
                     /locus_tag="PLB86_02325"
     CDS             6028..6885
                     /locus_tag="PLB86_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HRC64002.1"
                     /translation="MHVAFPGLGTVINIVAVVAGALLGMWVGHRIPERTRSVVTDCLG
                     LTTLLVAGLSTVSVTDAALRDAVGPGVPVLVVLGSLLIGSITGSTLRIEERLAGLAGI
                     LHERTARRRGGPATKADTGTSAAAAGDQTGTTDAVGDPETHADADAARERFIEGWLSA
                     SLLFCVGPLAILGSLSDGLGRGIDQLTLKSVLDGFAALAFASSFGVGVLLSAVSVGVV
                     QGLWTVLGVVLGSLLPDAHIAALTATGGLLLVGIGLRLLRIRDVPVGDMLPALLFAPL
                     LTQAIIALR"
     gene            complement(6938..7567)
                     /locus_tag="PLB86_02330"
     CDS             complement(6938..7567)
                     /locus_tag="PLB86_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC64003.1"
                     /translation="MTIPTPAPPESPSPDRSGALRIVVAEDEALIRMDLVEMLAEAGY
                     EVVGEAGDGEAAVELVREARPDLVLMDVKMPVLDGISAAAIIGKESLAPVVMLTAFSQ
                     KELVERASEAGVMAYVVKPFTSADLTPAIEVAASRWAQLRTLESEIADLADRLETRKL
                     VDRAKGLLMTSLKVSEPDAFRWIQKTAMDRRLSMKEVATLVVEGLPSKG"
     gene            complement(7766..7848)
                     /locus_tag="PLB86_02335"
     tRNA            complement(7766..7848)
                     /locus_tag="PLB86_02335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7813..7815),aa:Leu,seq:caa)
     gene            complement(7948..>9398)
                     /gene="pyk"
                     /locus_tag="PLB86_02340"
     CDS             complement(7948..>9398)
                     /gene="pyk"
                     /locus_tag="PLB86_02340"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRC64004.1"
                     /translation="LGPATSSPARIRELVLAGMDIARLNLSHGEYSAHEKVYRAVRQA
                     SDETGRAVGILVDLQGPKIRTGRFADGPVILKVGERFTITTLDVPGDQSRVSTTYKGM
                     PGDVHPGDLLLIDDGKVTLRAVEVTDTDIHTEVVEGGAVSNNKGINLPGVAVSVPALS
                     DKDKADLRWALSLRADMIALSFVRSAADIEDVHAIMDEVGVRLPVIAKIEKPQAVENL
                     PEILEVFDGLMVARGDLGVELPLEQVPLVQKRAVELARNRAKPVIVATQVLESMIENS
                     RPTRAEASDAANAILDGADAVMLSGETSVGAWPIDAVRTMARIIENTEEHGLERLAAL
                     HGQPRTIGGAVTLAAFEVGELINAKYLVTFTSTGDSAKRLARFRSKIPMLAFTSSPAT
                     RSQLALVWGIETYLGPSVEHTDQMAEQVEQALLAEGRCVEGDLIVMVAGSPPGIPGST
                     NMMRVLRVAGDTGHGNPFFHSHPGAEPVRSVQ"
     assembly_gap    9399..9498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9730..11190)
                     /locus_tag="PLB86_02345"
     CDS             complement(9730..11190)
                     /locus_tag="PLB86_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRC64005.1"
                     /translation="MADPQGFLNTRERELPTRRPVPIRLQDWREVYEDQDIHQLQRQA
                     GRCMDCGIPFCHNGCPLGNLIPEWNDLAWKGRWRDAIERLHATNNFPEFTGRLCPAPC
                     ETACVLGINQPAVTIKQVEVTVVEQAFAWGGVLPQAPERLSGKTVAVVGSGPAGLAAA
                     QQLTRAGHTVAVFERADKAGGLLRYGIPEFKMEKSIVERRIAQMEAEGTRFRTGVAVG
                     EDLTGEQLKARYDAVVLAIGATVPRDLSVPGRELSGIHQAMEYLPQGNRAALGQTVAG
                     QILATGKDVVVIGGGDTGADCIGTALRQGARSVTSLEILPQPPEERPAGQPWPTYPMI
                     FRVASAHEEGGERVYAVSTVEFVGEADGSVKALRVTDVALSNGRFEPVPGTERELPAQ
                     LVLLAMGFLGPQGEGLLEQLGVDLDERSNVVRNGSYMSSVPGVFVAGDAGRGQSLIVW
                     AIAEGRSAAHGVDAWLTGNPSQLPRPINPTDRQLLA"
     gene            complement(11183..15736)
                     /gene="gltB"
                     /locus_tag="PLB86_02350"
     CDS             complement(11183..15736)
                     /gene="gltB"
                     /locus_tag="PLB86_02350"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRC64006.1"
                     /translation="MSHPRMPFSAQPDDVGLYRRDFEHDACGVAMVATLRGTAGHDIV
                     ELALTALRNLDHRGATGSDPLVGDGAGILTQVPDRFLREVTGLPLPPVGGYAVGTAFL
                     PTNGAERADAIERIENIATQEDLRVIGWRDLPVTPGLVGDAARVVMPFFAQVFVESTQ
                     GRTGLELDRAAFCLRKRAEREVGVYFPSLSARTLVYKGMLTTGQLEPFFPDLSDPRFE
                     TELALVHSRFSTNTFPSWPLAHPYRLIAHNGEINTVKGNRNWMRARESMLATDLIPGD
                     LQRLFPICTPGASDSASFDEVLELLHLSGRSLPHAVLMMIPEAWESHASMDPARRAFY
                     AYHANLMEPWDGPACVTFTDGTLIGAVLDRNGLRPGRYSVTDDGLVVLASETGVLDLD
                     PAKVVRKGRLQPGRMFLVDTEHGRLVSDDEVKAELAAAQPYQEWLHAGNIELDDLPER
                     EHVIHTAKSVARRQQTFGYTQEELRIVLAPMARTGGEALGSMGTDSPIAVLSDRPRIL
                     FDYFTQLFAQVTNPPLDAIREELVTSLGQVIGPESNILADTPAHARKVIVKFPVIDND
                     ELARIVRINHDGDLPGYATHVVKGLYPVNGGAAAMTARIEQIFAEVSAAISRGVRFVV
                     LSDRDSNADLAPIPSLLLTSAVHHHLLREKTRTQVGLLIEAGDVREVHHVALLIGFGA
                     AAVNPYLAMESVEDMVRSGALTGITPEKAVANLIKALGKGVLKVMSKMGISTVASYCG
                     AQVFEAIGLSQELVDRYFTGTVSQLGGVGIDVLAAESAARHSVAYPAEGIHQAHRTLW
                     VGGEYQWRREGEPHLFDPETVFRLQHSTRQRRYDIFKQYTSRVNDHSERLMTLRGLFD
                     FADPSVTGRAPIDISEVEPVSEIVKRFQTGAMSYGSISKEAHETLAIAMNRLGGKSNT
                     GEGGEDEERLLDPTRRSSIKQVASGRFGVTSLYLSESDDIQIKMAQGAKPGEGGQLPG
                     NKVYPWVAKTRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARIHVKLVSEV
                     GVGTVAAGVSKAHADVVLISGHDGGTGASPLTSLKHAGAPWELGLAETQQTLRLNGLR
                     DRIVVQTDGQLKTGRDVVIAALLGAEEFGFATAPLVVSGCVMMRVCHLDTCPVGVATQ
                     NPELRARFSGKPEFIETFFEYIAEEVREYLAQLGYPTLQEAIGQVRSLVTDRAVSHWK
                     ASGMDLRPILAEVVDPSGGTAYQSVSQDHGLQKALDNQLVAMARPAIDDGEQVRGSIA
                     VRNVNRTVGTMLGHAVTSAHPHGLPDGTIELTLTGSGGQSFGAFLPAGVTLKLVGDTN
                     DYLGKGLSGGRLVVRPDRRAPLKAEQNVIAGNVIGYGATAGEIYLRGRAGERFCVRNS
                     GATAVVEGVGDHGCEYMTGGRVVVLGTTGRNFAAGMSGGLAYVLDLRRELVNAELVDL
                     APLRPGDVDAVREILEAHVQWTESAVATRLLANWEQEQSRFTVVVPRDYQRVLDVRAG
                     AQAEGLDPDGTEVWNRIMEASRG"
     gene            complement(15852..16823)
                     /gene="lgt"
                     /locus_tag="PLB86_02355"
     CDS             complement(15852..16823)
                     /gene="lgt"
                     /locus_tag="PLB86_02355"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRC64007.1"
                     /translation="MSAGALATLVTEIPSPSTGVWWLGPVPIRGYALSILLGIVVAVW
                     VAQRRIVSRGGTAGQVLDISAWAVPFGILGGRIYHLITSPQPYFGVGGNPWAAIRIWE
                     GGLGIWGAVALGALGAWIGCRRHGVAFGDFADAVAPGYLVAQALGRFGNWFNNEIYGP
                     PTDLPWGLRIYEWDQQAGHAVIDSAGNPMVKGVFHPTFLYEALWCLALAAFILWFERH
                     RRLRRGQAFALVVIGYPLGRFFIELMRTDDANRIFGQRVNVWVSLLVIALGVVLYRRT
                     GRAVDPVEAREPVEPVEPGEPVLAGEAGEAQVSGPDAQNSPASRHTE"
     gene            complement(16820..17728)
                     /locus_tag="PLB86_02360"
     CDS             complement(16820..17728)
                     /locus_tag="PLB86_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRC64008.1"
                     /translation="MSLQDPVGQPPEFPADPFAGAANVAGAAPVATPPPASSRRGVMG
                     VLVAAVAVLAIVVAVLLGQSMSRGGGTTASGAAPAANPAAPAATATAPAPAPAAKAPA
                     TATSKPAATGPVPTGAVGFGGPLVLNPSAPAGVPTLDLYEDPQCPICQQFEAIFGTAI
                     GEVAKNNEAKVVVHTMTFLDRNLRNDSSVRAANAAFCAADQGKFREYMSATFAAQPAQ
                     EGVGWTEAQLATIAQSVSITGSALDTWKTCQQGLTYAAHIAALEINSERSGVNGTPTA
                     LLNGKKMNLSGLDAAGFRAAVKAGTA"
     gene            complement(17797..18522)
                     /locus_tag="PLB86_02365"
     CDS             complement(17797..18522)
                     /locus_tag="PLB86_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRC64009.1"
                     /translation="MPRPDASAKLAATAPRRSRSGLAIIIAAVVFVAVVVAVVIGATT
                     SSKSSGGGGTAFPKGAASLGAPMVVNPEAPASVPVLDVFEDPQCPACATMDKVYGAAI
                     AELAKSNQAKVVIHTMTFLDTNLKNDASVRAAMGAYCAADQGKFLDYMSAVYVGQPQQ
                     EGVGWTAPQLEAFAMSARVGNLTTWQQCVKDKTYQAHTAAAAEAALKEGVNSTPTLRL
                     NGNKLTLTGNVNELAEAVKAATK"
     gene            complement(18568..19026)
                     /locus_tag="PLB86_02370"
     CDS             complement(18568..19026)
                     /locus_tag="PLB86_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRC64010.1"
                     /translation="MTRERGLDLLGTLLRLVLGGVILVAGALKVTNLGQSALAVRAYQ
                     LLPYDLAGYVGYALPIIEIVIGLLLVLGLFTRMSALLGALLMLAFVIGIASAWARGLS
                     IDCGCFGGGGTIGAEQTAYPLELSRDVALLLAGAWLVRRPHTAYALDDRI"
     gene            complement(19023..>19685)
                     /gene="trpA"
                     /locus_tag="PLB86_02375"
     CDS             complement(19023..>19685)
                     /gene="trpA"
                     /locus_tag="PLB86_02375"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRC64011.1"
                     /translation="AGADIIEVGIPYSDPLMDGPLIQRATEAALEGGSRVDDVFTAVR
                     AIADAGAAAYVMTYWNLVLKRGVARFAADLAAAGGSGLITPDLIPDEAGEWLAASDAH
                     GLDRVFLVAPSSTPERLTVATGACRGFVYAASTMGVTGTRETVGSAAQDVVRRTREVT
                     DVPVCVGLGVSTRAQAADVAGFADGVIVGSALVRALGEGGIPAMEALTRELAAGVREG
                     RP"
     assembly_gap    19686..19785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19786..19966)
                     /locus_tag="PLB86_02380"
     CDS             complement(<19786..19966)
                     /locus_tag="PLB86_02380"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRC64012.1"
                     /translation="MSPGTQVLAACRAEGRAALIGYLPVGFPSVEVSIEAMVAMVRAG
                     ADIIEVGIPYSDPLMD"
     gene            complement(20014..21216)
                     /gene="trpB"
                     /locus_tag="PLB86_02385"
     CDS             complement(20014..21216)
                     /gene="trpB"
                     /locus_tag="PLB86_02385"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRC64013.1"
                     /translation="MTTPHGRFGDFGGRYVPEALIPALEQLDEVRRKAAVDPEFIGEL
                     DRLHRTYTGRPSIITEVPRFAEQCGGARVILKREDLNHTGSHKINNVLGQALLAKRMG
                     KSRVIAETGAGQHGVATATAAALLGLDCVVYMGAVDTKRQALNVARMRLLGAEVVAVT
                     NGSQTLKDAVNEALRDWVANVDSTHYVLGTVTGPHPFPEMVRDFHRIIGVEARAQVLD
                     LVGRLPDAVLACVGGGSNAMGIFHRFLFDDSVRLIGFEAGGDGIESGRHAARFSGGTR
                     GVLHGAATYVLQDDDGQTIESHSVSAGLDYPSVGPEHAHLFETGRAEYRPITDDEAME
                     AFRLLCRTEGIIPAIESSHALAGAMKVGRELGPDALLLVSLSGRGDKDVHTAARWFGL
                     LDDAEGMP"
     gene            complement(21213..22073)
                     /gene="trpC"
                     /locus_tag="PLB86_02390"
     CDS             complement(21213..22073)
                     /gene="trpC"
                     /locus_tag="PLB86_02390"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRC64014.1"
                     /translation="MPATPTVLDEIIAGVRLDLADRVARTSLADLEARIATMPPALDA
                     EAALRAAPGIAVIAEVKRASPSKGALADIPDPAALATAYAAGGATAISVLTEERRFSG
                     SLADLDAVRATVRIPVLRKDFMVEPYQLLEARAHGADLILLIVAALSDIQLIAMREQA
                     QRLGMTALVEVHDAEETRRAVATGARVIGVNARNLKTLEVDPSAFARLRPLIPDEAVA
                     VAESGIRGVADVKGYAAEGARAVLVGEALVIGGDPAGAVASFAGVPVGGIPGQPQAST
                     TQASTTEGPR"
     gene            complement(22097..22474)
                     /locus_tag="PLB86_02395"
     CDS             complement(22097..22474)
                     /locus_tag="PLB86_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HRC64015.1"
                     /translation="MQPALIAVGAAAGAAYLYAVDPNAPGHYPPCPFLAVTGLWCPGC
                     GSLRALHALLHLDVGTALARNPFAVLASIYIAWTFYRWAYRRVTGTPLRLAPGWVITV
                     VWVSIVAFWILRNVPGMTWLSPL"
     gene            complement(22540..22908)
                     /locus_tag="PLB86_02400"
     CDS             complement(22540..22908)
                     /locus_tag="PLB86_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HRC64016.1"
                     /translation="MSYGSTPPPPPPGSGGYGAQPPMGPAGGMPYGGAPMSPPPQNNL
                     VLAILATVLCCLPLGIVAILKANKVNTLWAQGDQAGAHAAAAEAKKWSIISAILGIVV
                     GIIYFVFVFLAAANGSVTVR"
     gene            complement(23055..23294)
                     /locus_tag="PLB86_02405"
     CDS             complement(23055..23294)
                     /locus_tag="PLB86_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGxxPAAW family protein"
                     /protein_id="HRC64017.1"
                     /translation="MAGNDHHDNHGQSVAAWTGVAILTVAAAIMCWAVVVASTGLFIA
                     GAVLAVIGVVTGKVLAMAGFGVQKPGRNQVDSGVS"
     gene            complement(23364..23948)
                     /locus_tag="PLB86_02410"
     CDS             complement(23364..23948)
                     /locus_tag="PLB86_02410"
                     /inference="COORDINATES: protein motif:HMM:NF021079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HRC64018.1"
                     /translation="MTKRLVVLAVGGPLAVLLAALGQVWASGSSQDPVLGRAALSITG
                     TQMVPAALGLALVVGAALVAMLTGGPRIRTAAGVLMALAALAVVGLIGASLGSAGAAL
                     GRRAAELAGRTGSPVPVTSALGPWAWIAATGAGLTAVASIVATFSARRWAGLSARFDR
                     GGVDGATDRGARRSDWDDLTEGHDPTRADSGQGT"
     gene            complement(23945..25495)
                     /locus_tag="PLB86_02415"
     CDS             complement(23945..25495)
                     /locus_tag="PLB86_02415"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRC64019.1"
                     /translation="MSDIHAELTFGSTWPSLDVFGELARTRRVIPVVRRLLADGETPL
                     GVYRKLAKGEPGTFLLESAEHGGVWSRYSIVGAHSAATLTERDGQAHWIGMPPAGVPT
                     DGDPTIALRDTVAALATARIGGLPPLTGGMVGAITYDAVRRWEKVPDTGRDELNLPEL
                     AMVLATDLAVLDHSDGSVLLVANAINYDDTDERMEWAWRDAVARLDRMTAELGEPAPS
                     TVAVIEPVTLTVTSSHEPEAYLDNVERCKESIRAGDAFQIVVSQRFSTPCDADPIDVY
                     RALRAANPSPYMYLLRLPHPDGTAYDIVGSSPEALIKVTGREAITHPIAGSRPRGKTP
                     EDDVRLADDLAADTKERAEHVMLVDLSRNDLQRVCRAGTVDTVEFMTVRRYSHIMHLE
                     STVVGELKEGVAAYDVLVATFPAGTLSGAPKPRAMALIDEYEGLKRGIYGGVVGYLDF
                     AGDLDMAIAIRTAVIRNGVAHVQAGAGIVADSVPQLEHEECQHKAAAVLRAVQAAAGM
                     QAAVAVSP"
     gene            complement(25488..25868)
                     /gene="hisI"
                     /locus_tag="PLB86_02420"
     CDS             complement(25488..25868)
                     /gene="hisI"
                     /locus_tag="PLB86_02420"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRC64020.1"
                     /translation="MAAVSLRASSLDPAVAALLKRDDQGLVAAIVQQHDTGEVLMLGW
                     MDDEALARTLTTGRVTFWSRSRGEYWRKGDTSGHVQHVRSVAVDCDGDALLVRVDQVG
                     AACHTGDRTCFDARPLAVVGGHDE"
     gene            26000..27808
                     /gene="pabB"
                     /locus_tag="PLB86_02425"
     CDS             26000..27808
                     /gene="pabB"
                     /locus_tag="PLB86_02425"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRC64021.1"
                     /translation="MREAFVDFPWAPAGAAGPLAFGAPVEIVEARCLDEVRPALDRVA
                     AACADGRYAVGFVSYEAAPAFDPAMAVRSGSGVPLVWFGLHDAPLAEGAAREPAAYAC
                     SVWEAGTDEVTYAASVAAVREAIASGDTYQVNHTMRLRARFDGDPLGAWADLRRAQRD
                     GWFAYVDTGAHAVASVSPELFFTWDGHEVVTRPMKGTRARSRDGALDAARAADLRASA
                     KDRAENLMIVDLLRNDLGRVCRPGSVRVPSLFDVEAYPTVWQMTSTVTGESRPGLGLG
                     DLFGGLFPCGSITGAPKIRTMALITELEDRPRGVYCGAVGVVFPGGAAAFNVAIRTLV
                     VDQADGRARYDVGGGVVWDSDGRDEHAEALAKAAVLDMTQRDFALLETLRVETDEVVL
                     LDRHVERMARSAEWFGWPFDPDRARALVRSDGAGPARARLLLRPSGDLRVEFTSLTDI
                     FGMPPAPDRTAPPPRAVVLARSPVWSGDPWLRHKSTRRGVYERHRREATAYLQAAGWD
                     AGAPFDVLLWNERGELTELTIGNLVADVAGTLVTPPVACGLLPGTLRAHLLASGTVSE
                     RVLRVQELPSATALWLVNAVRGWVPITLVEPPGQPT"
BASE COUNT         4257 a         9400 c         9749 g         4236 t
ORIGIN      
        1 gcgaaaccga accagaacgg agccgaccag ccccacgcgt tgagcaggaa ggtggtgatg
       61 aagccacccg tcgccgcgcc ggccgggagg ccgacaccgt agacgcccat cgcggtgcca
      121 cgcttggcat cggaggagta ctccgagacc aggatgttga ggctggggat gagcgcaccg
      181 atccacagac cggcgaagaa ccgcgcggcc agcaggaacg ggaagctcga ggtcggcgcc
      241 aacgccgagg ccgccatgcc cacacagatg agggccaggc acatgaggat gtgcttgcgc
      301 cgcccgatcc ggtcggccag cggcgagatc aagatcgcgc cgaggcccat cccgaaggtg
      361 ccggcgctga ggaagtagcc gatgtcggcc gcgttgggca gggcgaacgc cttggccacg
      421 gcggtggacg tgaacgcggc gacgagcacc tcgaacccgt cgacgaaggc gagggcgata
      481 caaatgccaa tgatgcgctt ctggaacgct cgcatcggtt gtgaggcgat ggattcacgt
      541 aggttcatct aaggaccttc cacgaacagg gtgggtgcaa cggagggtag tgctgggcta
      601 gttggtcgtt cctaccgacg gtgagcaagt tccttcttga gaatcttgcc cgtggcgctc
      661 ttgggcatct ctgcgatgac ctcgacaatc cgcgggtact tgtatgccgc gaggcgctcc
      721 ttgacgtaat caatgaactc ctctggggtg cctgcctcac cgggcttgag agtgaggtag
      781 gccttgatct cctcgcccag gcgctcgtcg ggcacgccga cgacggcagc ctccgagatc
      841 ttgggattgg tgtagagaac ctcttcgacc tcgcgggggt acacgttgta tcccccccga
      901 atgatgagtt ccttcttgcg gtcggcgaag aacaggaatc cttcttcgtc catccacgcc
      961 aggtcgccgg tgtgcagcca gccattggtg aacgctgctg ccgtggcttc cgggttgttc
     1021 agatagccgg acatcgtgtt gtgcccgcga atcatgatct cgccgatcga caacttgccc
     1081 cgaggcagtc gtcgcttctg ctcgtcccag atctcgatgt cgacgcccgg gatcgcctta
     1141 ccgatgctgt acggcttgcg gtcacgcggc atgttgatcg tcgcaaccgg gccggactcg
     1201 gtgaggccgt acatctctag gatctgcacg ccgaaggtct tctcgacctg gtcgatgatc
     1261 tccgccgcga tggccgcacc ggccgacccg cagaacgcca gggtcgaggt gtcgaagttg
     1321 gccagggtcg ggtggttgag cagcgagtgg tacatcgtcg gaacgccgtg gatgtgcgtg
     1381 gcgccctcgg cctggatcgt ctggagcacc ttctcggtgt cgaacttcgg caggagcagc
     1441 accgtgccgc cggcccggac cgtcgcgttg aggatgcctg acaggccgag cgcgtggaac
     1501 agcggcatga ccgcgatgat cttgctcgtc gggcccatct cgaaggcacc gatgtgggcg
     1561 ccggcgttca tgtagagctg gaagtgggtg agctgaacgc ccttgggctt gccggtcgtg
     1621 cccgacgtgt aaagcaggac ggcagtgtca ccggggtcgc gctggataaa cggcttcggg
     1681 tcggggaccg ggtggtcggc caactcagag aacggccgtg ccacgactcc gtcgggcaat
     1741 gactcgggga ccgtcccgag gacgtacaac gtcgccccgc cggcctgggc ggccgcctcg
     1801 gcagcctcac ccgccgacgc gtcgacggtg atgacgtgct tggccgaggc gttctccagg
     1861 acgtaggaca actcgccggc cttgtagagc gtgttggtcg gaatgaccac gccaccggcc
     1921 ttgaggatgc cgtgcaggga gatgacgaac tccggaacgt tcggcaactg caacatcacg
     1981 gcgccgccgg gctccaaccc cgcagccatg aggccggcgg caaccgcgtc ggatcgccgg
     2041 tcgagctcgc cgtagctgat ccgctccgaa ccggtcacga tcgccgggtg ctcttgatct
     2101 cgctgggcag agtcccgcag gatcgaagag aggttgaagg tcatgcgtcg tcctcctgaa
     2161 gaagtggggt ggcgcgatgg tagagggcag ggtggtgcga caaagggtcg tgctgtgaat
     2221 cggaattgcg agtaactgtc agttggtcgg gggttcccag ccccacaacc agtccgcgta
     2281 ggaatagtcg tcgtcagtga tgccgcggaa gagttcgttg cgcaggagtg cgccggtgcc
     2341 gttgatgtgc caaatatcgc cccagacgcg agcggaccgc tggacgcggg ccgtgcgggg
     2401 ggcgcggatc cgttggtatg ccgcgagcgc ccggtccact cccccgtcgt cggccgtcgc
     2461 accgtcgtgc gcggcgaact gctgggcgag ggtggcggcg tcttcgatgg cctggcaggc
     2521 gccctgggcg aggtattgga gcatggcgtg ggcggcatcg ccgagcagcg ccgtgcgtcc
     2581 gtcgacccac gtgtcgaccg gctcgcggtc gaaggtcggc cagcggaagt tgcgctgcag
     2641 ggctggcagg gccacccgca ctgctgggca cataccggag aacgccgcgt ccaactcgtc
     2701 ggggccgccc caatcggggt tgccggtggc gaaactcggg ctcttgaaga cggccacctg
     2761 gttgtagagg tcgccgcgcc ggatcgggta ctgcaccagg tgcaggccag gcccgaccca
     2821 ggagatgacg gtatcgaggt cgtggggccg ttcgacctgc tcgatcggca gggcgccccg
     2881 gtaggcgaca tagccggagg cgatcggctc ggatgcgccg tggatcgctt cgcgcaggtg
     2941 ggagcgcaac ccgtcggccg cgatgacggc gcgcgcggca tactcgctgc cgtcggagca
     3001 gacgacgcgc gtctcccctc ccacggtctc ggtccgcacg actgccttgc cgttctccag
     3061 cacgacgccg gcggagcggg cggcctgcag cagcacgtcg agcaggtcgc cgcggtggac
     3121 gacgagatag ggcgagccga aggtgcgcag gaaccggtca ccgaggtccg ccgagaccag
     3181 gccctcaccc gtgcgggcat ccgcgacgac gatccgggcg ggtgcgacac cgacgtcgag
     3241 cacggcgtcg agcaccccga gccggcgcag gatgccggtc gcattgggag ccaactgcag
     3301 gcccgcgccc acttcggcga agcgctcggc gcgttcgaga acgcgcacgg tgcgcccgtc
     3361 gcgagccaga gcaagggcag tcgtcaaccc gccgatgccg cctccgacga cgacgatctc
     3421 gcgtgcggac tcagtcgtcc cctcgcccat cgcgtgcctc cttgccagcg ggttaatagt
     3481 tacactctag aaaggttgag attgtcaagg aatggactag cggcgatttt ccgtcgtgcg
     3541 gaaaccgccg cccgctgtcg gccggacggc cggcttgcta gcgtgacccg cactggcgca
     3601 gcgacgagga ggcgatgtga aggcgaccca actcaccgcg ttcgatgcgt ccttcttggc
     3661 cctggacacc aacatcagcg tgggtcacgt gacgttgctg tcgctgctgg accgcgaggt
     3721 ctcccccgac gagttgttcc accagatcga ggcccacctg cacttggcac cgatgctgcg
     3781 ccgcaaactg cgggcgccct cactcatcgt ggggcgaccg tggtgggtcg atgaccccga
     3841 cttcgacctc gatgaccacc tcaccttcac cgacctgggt ggtcacgccg acgaggccgc
     3901 gatgtgcgcc gaagccgtcc gcttctcccg cctgcacctg gaccgctccc gacccctctg
     3961 ggagctgctg ctcatcggcg ggatggccga cggcaccggg gcgttggtgt cgaagatcca
     4021 ccactgcgcg gtcgacggtg tgggcaagcg ggacctgctg tctttgctgt ttggcaccga
     4081 gagcagcctt gacccgcacc ggcggtggcg gcccgacggt gggcccggcc agttgtcccg
     4141 catcacccat cgggtcaccg aagccaccga acgcacgggc gctgccctgc gtctggagtt
     4201 ccaggccgtg acggctgccc ccgccgccgt gctcaaggcg acgcgcgaca cggtcacccg
     4261 actggcagac gggctcgagc gcttcgtcga gggtgccgaa agcgtcgagg ccgagcccgc
     4321 ctgggcccgg gtcggctcgc cgcccgccac cccgttcaac gcggcgatca ccgccgaacg
     4381 cgcgtgggcc tggctgccga tgccggtggc cgcgagttcc gcgctgcgac gcgcgacggg
     4441 cagcaccctc aacgacgtcc tcatggcggc gacggccggt gccctgcggg actggcttgc
     4501 cgagaacgac gccttgacga cggaccctct gatcgccatg gtccccatgt cgatccgcgg
     4561 ccagggccac agcgtcggca gcaaccagat catgctcacg ttgtgtcccc tgccgaccca
     4621 cctcgacacc ccgcgcgaac ggctggcctt cgcgcactcg gccatggacc acgccaaggc
     4681 gacgccgacc tacccctcga cgttgatgtc cgacctggcc gccgtggccg gcccgacggt
     4741 gacccaactg ctcacccagg ccgccgccat ggtgcggctc gccgaccgtg tccagttgcc
     4801 gttcaacctc atggtctcga acgtgccagc gccggcccgt gagttcatcg tcggtcgcgg
     4861 cgcccgggtg atcggctcgt tcccgttccc tccgttgtcc gacggcatgg gcctgtcggt
     4921 gtgcgcccag ggctatccgg gggctttgga catcggcatc gggtcgtgct cggacctgct
     4981 gcccgaccca tggcgggttc gcgacctgtt ggagcaggcc cacgaggacc tcgtcgcgtc
     5041 ggcctagccg tcggtccccc gtgctcttcg ggccnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnccacga ggacctcgtc gcgtcggcct agccgtcggt cccccgtgct
     5221 cttcgggcca atgccccgtc agtgcgcggc gaaccgaccc tgagcggccc ggatccgggc
     5281 gacccggcga cgggccagca cctggtcgag ggcgaagcga gactcggcgg tgtcgccgac
     5341 caggcgccgt tgcagagcgg ccgtgagcgt gacccgacgg atggtgtgcg gagtgaagcc
     5401 gagccgggca aagaaccggt tcgagtcgcg caccgccgac ggcacactgc aagcgatctg
     5461 gtcggcgccg tgccgatcgg cgaaggttgc cacggccgtc atcagcgccc gcgcgacacc
     5521 cttcttgcgg gcatccttgg cgacatagat ctgatcgacc gacacacacg ccgcatcgct
     5581 gaacgggttg agcgagcagt cgctaagcac gacgtaaccg acggccgcac cgttgaggta
     5641 tgccgcgaac gcgacggtct caggccgcag cagggcggcc cggaccacgt cagccgtcac
     5701 ccggcgaagc cccaactcgg tcggggcccg ctcttcgcgc tgttgcgccc aaaggtcgca
     5761 gacgacatcc accgagcgtt cgtcgacccg acgaaccagg gcttgggggg cctggggagc
     5821 tgacaccgat gtccttccac gaacgcgcga ccggtgtctg gcccctgacc ggtgagagcc
     5881 tctcggccca agttgactca ctgtgttccc gctgatgacg cactttgttc ccgctgacaa
     5941 ccgactctaa tactcctcgg cacccaccgg tacccccggg ttgggcgctg cgacagccgc
     6001 tctcacgcga cccatagggt gtgggccatg catgtcgcct tcccgggcct gggcacggtc
     6061 atcaacatcg tcgcggtcgt ggccggtgcg ctgttgggca tgtgggtcgg gcaccggatc
     6121 cccgagcgca cccgatcggt cgtcaccgac tgccttggcc tgacgacgct gctcgtcgcc
     6181 gggctgtcaa cggtctcggt caccgatgcc gcgttgcgcg acgctgtcgg cccgggagtg
     6241 cccgtgctcg tcgtcctcgg ctcgttgctc atcgggtcga tcaccgggtc cacgctgcgc
     6301 atcgaggaac ggctggccgg gttggccggc atcctgcacg aacgaaccgc ccgtcgtcgc
     6361 ggcggcccgg cgaccaaggc cgacaccggc accagcgcag ccgccgccgg cgatcagacc
     6421 ggcaccaccg acgcggtcgg ggaccccgaa acgcacgccg atgccgatgc cgcccgggag
     6481 cggttcatcg aggggtggct cagcgcgtca ttgttgttct gtgtcggccc gttggccatc
     6541 ttgggcagcc tgtccgacgg cctgggccgg ggcatcgacc aactcaccct caagtcggtg
     6601 ctcgacgggt tcgccgccct agccttcgcg tcgtcgttcg gcgtgggcgt gctgctctcg
     6661 gccgtctcgg tgggggtcgt ccaaggcttg tggacggtgc tcggcgtggt cctcggctcc
     6721 ttgctccccg acgcccacat cgccgcgctg accgcgaccg gcgggttgtt gctcgtcgga
     6781 atcggactgc ggctgttgcg gatccgcgac gtgccggtcg gcgacatgct gccggccctg
     6841 ctttttgcac ccctgctgac gcaagcgatc atcgcgctgc gctagccgcg ctcgctgcgt
     6901 tcgccgcgcg agcggcacat cgcgtgggct gggttgctca gcccttgctc ggcaggcctt
     6961 cgacgacgag ggtggctact tccttcatcg acaggcgtcg gtccatcgcc gtcttctgga
     7021 tccaccggaa cgcgtccggc tcactcacct tgagtgaggt catgagcagc cccttggccc
     7081 ggtcgaccag tttgcgggtc tcgagtcggt cggccaggtc ggcgatctcg gactcgaggg
     7141 tgcgcagttg cgcccagcgg ctggcggcga cctcgatggc cggcgtgagg tcggccgacg
     7201 tgaacggctt gacgacgtag gccatgactc cggcctcgct ggctcgctcg acgagctcct
     7261 tctgcgagaa ggcggtcagc atgacgaccg gcgcgagtga ctccttgccg atgatcgccg
     7321 cggcgctgat cccgtcgagc accggcatct tcacgtccat gagcaccaga tccggacgcg
     7381 cctcgcggac caactcgacg gctgcctcgc catcgccggc ctcaccgacg acctcgtagc
     7441 ccgcctcggc gagcatctcg accaggtcca tccggatgag ggcttcgtcc tcggccacga
     7501 cgatccgcag tgcgccggat cggtccggcg acggggattc cggtggggcg ggcgtcggga
     7561 tcgtcatggc cagatcctac cgagcctgcg caacgccgat tgacccagtc caggaccccg
     7621 cggatgactt ggcggcctcg tcctcgcggg tgtgcgcagg caaccccggt ccggacgagt
     7681 gggcgggcag ccgggcctca cggccgccgc gtccaccacg atcgcaacta cccaagctca
     7741 acgacacatc tcaagaacac aacggtgccg ggagggggat tcgaaccccc acgcccttgc
     7801 gagcagagca ttttgagtgc cccgtgtctg ccattccacc accccggctg ggtgcccgag
     7861 aagcataccc acagcctccc ccacaccaac aaaccgctca ggtatgccgc gtggccgggc
     7921 cgcgcggcat acctgagtcg tcgtgcgcta ctggaccgaa cgaaccggct cggcgccggg
     7981 gtgcgagtgg aagaaggggt tgccgtggcc ggtgtcgccg gcgacccgca gcacgcgcat
     8041 catgttcgtc gagccgggga ttccgggcgg ggaaccggcc accatgacga tgaggtcacc
     8101 ctcgacacag cggccttcgg cgagcagggc ctgctcgacc tgctcggcca tctggtcggt
     8161 gtgctcgaca ctcgggccga ggtaggtctc gatgccccag accagtgcca actggctgcg
     8221 cgtcgcgggg ctggaggtga aggccagcat ggggatcttg gagcggaacc gcgccaaccg
     8281 cttggccgag tcgccggttg aggtgaacgt cacgaggtac ttcgcgttga tcaactcacc
     8341 gacctcgaag gcggccagcg tgaccgcccc cccgatggtc cgcggctggc cgtgcagcgc
     8401 cgcaagacgc tccaggccgt gctcctcggt gttctcgatg atccgggcca tggtgcgcac
     8461 tgcgtcgatg ggccacgcgc ccacgctcgt ctcgcccgag agcatcaccg catcggcccc
     8521 gtcgaggatc gcgttggccg cgtcgctggc ctcggcccgg gtcggtcgcg agttctcgat
     8581 catcgactcg agcacctggg tggcgacgat gacgggcttg gcccggttgc gcgccaactc
     8641 gacggcgcgc ttctgcacga gcggcacctg ctccagcggg agttcgacgc ccaggtcgcc
     8701 acgggccacc atgagcccgt cgaagacctc gaggatctcc gggaggttct cgacggcctg
     8761 cggcttctcg atcttggcga tgaccggcag ccggaccccg acctcgtcca tgatcgcgtg
     8821 gacgtcctcg atgtcggcgg ccgagcggac gaacgacagc gcgatcatgt cggcccgcag
     8881 ggacagtgcc cagcgcaggt cggccttgtc cttatccgac agtgcgggga cgctcacggc
     8941 gacgccgggc aggttgatgc ccttgttgtt cgacaccgca ccgccctcga cgacctcggt
     9001 gtggatgtcg gtgtcggtca cctccacggc gcgcagggtg accttgccgt cgtcgatgag
     9061 caacaggtca ccggggtgaa cgtcgccagg catgcccttg taggtcgtgc tcacccgcga
     9121 ctgatcgccg ggcacgtcga gggtcgtgat cgtgaaacgc tcgcccacct tgaggatcac
     9181 tgggccgtcg gcgaaccgac cggtgcggat cttcggaccc tgcaggtcga caaggatccc
     9241 gacggcccgc ccggtctcgt cggaggcctg gcgtaccgcc cgatagacct tctcgtgcgc
     9301 ggagtactcc ccgtgggaga ggttgagccg ggctatgtcc attcccgcga ggacaagttc
     9361 ccggatccgg gctgggctgg aggtggcagg gccaagagnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnncc cgcgaggaca agttcccgga tccgggctgg gctggaggtg
     9541 gcagggccaa gagtgcaaac gattttagcg cgacgcatgt cgagcaacct taggggttag
     9601 tgacgcggag cggggccagg tatgccggtc ccggcaggtc taatccggat cagacccctg
     9661 ttctgatccg gatcagactc gacttgtgcg agatatgcga gatatgtgga gagttggtgg
     9721 tcgaccgggt caggcgagca actgtcggtc ggtcgggttg atcggtcgag gcaactggct
     9781 ggggttcccc gtcagccagg cgtcgacccc gtgcgctgcc gagcggccct cggcaatggc
     9841 ccagacgatg agggactgac ctcgaccggc gtcaccggcg acgaagacgc ccggcaccga
     9901 gctcatgtag ctgccgttgc gcacgacgtt cgagcgctcg tccaggtcga cgcccaactg
     9961 ctcgagcagc ccctcgccct gtgggccgag gaagcccatg gccagcagga ccaactgcgc
    10021 cggcaactcc cgctcggtgc ccggcaccgg ctcgaaccgg ccgttggaca acgcgacgtc
    10081 ggtgacccgc agcgccttga ccgacccgtc cgcctcgccg acgaactcga cggtcgacac
    10141 ggcatacacc cgctcgccgc cctcctcgtg cgcggaggcg acgcggaaga tcatcgggta
    10201 ggtgggccac ggctgccccg ccgggcgctc ctccggcggc tgcggcagga tctccagcga
    10261 cgtcaccgac cgcgcgccct ggcgcagtgc ggtgccgatg cagtcggcgc cggtgtcgcc
    10321 gccgccgatg acgacgacgt ccttgccggt cgcgaggatc tgacccgcga cggtctgccc
    10381 cagcgcggca cggttgcctt gcgggagata ctccatggcc tggtgtatgc cgctcagctc
    10441 gcgcccgggg acgctcaagt cacgcgggac ggtcgccccg atggccagca ccacggcgtc
    10501 gtagcgggcc ttcaactgct caccggtgag gtcctcgccg acagcaacac cggtgcggaa
    10561 ccgggtgccc tcggcctcca tctgggcgat ccgacgctcg acgatggact tctccatctt
    10621 gaactcgggg atgccatagc gcagcagccc gccagccttg tcggcccgct cgaaaacggc
    10681 gaccgtgtga ccggcccggg tgagctgctg ggccgctgcc aggcccgctg gacccgagcc
    10741 gacgacggca acggtcttgc cggacaaccg ctcgggtgcc tgcggcaaca ccccgcccca
    10801 ggcgaaggcc tgctcgacga cggtgacctc gacctgcttg atcgtcacgg cgggctggtt
    10861 gatccccagg acgcaggcgg tctcgcacgg tgccgggcac aaccgcccgg tgaactcggg
    10921 gaagttgtta gtcgcgtgca gccgctcgat cgcgtcgcgc caccgcccct tccacgccag
    10981 gtcgttccac tcggggatga ggttcccgag cgggcatccg ttgtgacaga acggaatccc
    11041 acagtccatg cagcgaccgg cctggcgctg caactggtgg atgtcctggt cctcgtagac
    11101 ctcacgccag tcctgcaacc ggatcggcac ggggcgacgg gtgggcaact cgcgttcgcg
    11161 agtgttgaga aagccttggg ggtcagccac gggacgcctc catgattcgg ttccacacct
    11221 cggtgccgtc cgggtccagg ccctcggcct gggcaccggc gcgcacgtcg aggacccgct
    11281 ggtagtcgcg ggggacgacg acggtgaaac gggactgttc ctgctcccag ttggccagga
    11341 gccgggtcgc caccgccgat tcggtccact gcacgtgcgc ctcgaggatc tcccgaaccg
    11401 catcgacatc gccggggcgc agcggggcca ggtcgacgag ctccgcgttg accagctcgc
    11461 ggcgcaggtc cagcacgtag gccaacccgc ccgacatgcc cgcggcgaag ttgcgcccgg
    11521 tggtgccgag cacgacgacc cgaccaccgg tcatgtattc gcatccgtgg tcaccgacgc
    11581 cttcgacgac cgccgtcgcg ccagagttgc ggacacagaa gcgctcgccg gctcgaccac
    11641 gcaggtagat ctcgccagcc gtcgcgccgt aaccgatgac gttgccggca atgacgttct
    11701 gctcggcctt gagcggcgcc cgacggtccg gacgcacgac gagccgtcca cccgagaggc
    11761 ccttgccgag gtagtcgttg gtgtccccca ccagcttgag ggtgaccccc gcgggcagga
    11821 acgcaccgaa ggactgaccg ccggacccgg tgagcgtgag ctcgatggtg ccgtcgggca
    11881 ggccgtgtgg gtgtgccgac gtgaccgcgt ggccgagcat cgtccccacg gtgcggttga
    11941 cgttgcggac cgcgatcgac ccgcgcacct gctcgccgtc gtcgatggcc ggtcgggcca
    12001 tcgccacgag ctggttgtcc agcgccttct gcagtccgtg gtcctgcgac acgctctggt
    12061 atgccgtgcc gccactgggg tcgacgacct cggcgaggat gggacgcagg tccatcccac
    12121 tggccttcca gtgcgagacc gcccggtcgg tgaccaggga acgaacctga ccgatcgcct
    12181 cctgcagggt cgggtaaccc aactgcgcga ggtattcgcg gacctcctcg gcgatgtact
    12241 cgaagaaggt ctcgatgaac tccggcttgc cggagaagcg ggcccgcagt tccgggttct
    12301 gagtcgccac gccgaccggg caggtgtcca ggtggcagac gcgcatcatc acgcagccgc
    12361 tgaccaccag gggtgcagtg gcgaagccga actcctcggc gcccagcagc gccgcgatga
    12421 cgacatcccg gccggtcttg agctggccgt cggtctgcac gacgatccgg tcgcgcagtc
    12481 cgttgagccg cagggtctgc tgggtctcgg ccaggccgag ctcccacggt gcgcccgcgt
    12541 gcttgagcga ggtcaacggc gaggcccccg tgccgccgtc gtggcccgag atgagcacga
    12601 catcggcgtg ggccttggag acacctgccg cgaccgttcc gacacccacc tcggacacga
    12661 gcttgacgtg gatccgagcc gccgggttgg cgttcttgag gtcgtggatg agctgggcca
    12721 ggtcctcgat cgaatagatg tcgtggtgcg ggggcgggct gatcagcccg acacccggcg
    12781 tcgagtgccg agtcttggcc acccaggggt agaccttgtt gccgggcagc tggccgccct
    12841 cccccggctt ggcgccctgg gccatcttga tctggatgtc gtcggattcg gacaggtaga
    12901 ggctggtcac gccgaagcgt ccggaggcca cctgcttgat cgacgagcgc cgagtcgggt
    12961 cgagcagccg ctcctcgtcc tcgccgccct cgcccgtgtt ggacttgccg cccaagcggt
    13021 tcatcgcgat ggcgagggtc tcgtgcgctt ccttgctgat cgacccgtac gacatcgccc
    13081 cggtctggaa gcgcttgacg atctcgctga ccggctccac ctcgctgatg tcgatgggcg
    13141 cccggcccgt caccgacggg tcggcgaagt cgaacagacc gcgcagcgtc atgagccgct
    13201 cggagtggtc gttgacccgc gaggtgtact gcttgaagat gtcgtagcgc cgctgccgcg
    13261 tggagtgctg cagccggaag accgtctcgg ggtcgaacag gtgcggttcg ccctctcgac
    13321 gccactggta ttcgccgccg acccacaggg tccggtgggc ctggtggatg ccttccgccg
    13381 gataggccac cgagtgccgg gctgccgact cggccgcgag gacgtcgatg ccgacgccac
    13441 cgagttgcga caccgtgccg gtgaagtagc ggtcgaccaa ctcctgggac agcccgatgg
    13501 cctcaaagac ctgtgccccg cagtaggacg caaccgtcga gatccccatc ttgctcatca
    13561 ccttgagcac acccttgccc agggccttga tgaggttggc gaccgccttc tcgggcgtga
    13621 tcccggtcag tgcgccactg cgaaccatgt cctcgacgga ttccatcgcg aggtacgggt
    13681 tgacggcggc agcgccgaaa ccgatgagca gagcgacgtg gtggacctcg cggacgtcgc
    13741 cggcctcgat gagcagaccc acctgggtgc gggtcttctc gcgcagcagg tggtggtgga
    13801 ccgcggaggt gagcaacagc gacgggatcg gcgcgaggtc agcgttggag tcgcggtcgg
    13861 agagcacgac gaagcgcacc ccgcgcgaga tcgcggccga gacctcggcg aagatctgct
    13921 cgatccgcgc cgtcatggcg gccgctcccc cattgacggg gtagaggccc ttgacgacgt
    13981 gggtggcgta acccggcagg tcgccgtcgt ggttgatccg cacgatccgg gccaactcgt
    14041 cgttgtcgat gaccgggaac ttcacgatga ccttgcgcgc gtgcgccggg gtgtcggcca
    14101 ggatgttgct ctccgggccg atgacctggc cgagcgaggt gacgagttcc tcgcgaatgg
    14161 cgtccagcgg cgggttcgtc acctgtgcga agagctgggt gaagtagtcg aagaggatgc
    14221 gcggccggtc ggacagcacc gcgatcggtg agtcggtgcc catcgacccg agggcttcgc
    14281 cgccggtccg cgccatcggg gcgaggacga tccgcagctc ctcctgggtg tagccgaagg
    14341 tctgctgacg gcgagccacc gacttggcgg tgtggatgac gtgttcgcgc tcaggcaggt
    14401 cgtccagctc gatgtttccg gcgtgcaacc actcttggta gggctgtgct gccgcgagct
    14461 cggccttgac ctcgtcgtcg ctgaccaggc ggccgtgctc ggtgtcgacg aggaacatcc
    14521 gacccggctg caaacgtccc ttgcgcacga ccttggccgg gtcgagatcc aggacaccgg
    14581 tctccgacgc aagcacgacg agcccgtcgt cggtcaccga atagcgccca gggcgcagcc
    14641 cgttgcggtc aaggacggca ccgatgagcg tgccgtcggt gaaggtcacg caggccggcc
    14701 cgtcccacgg ctccatgagg ttcgcgtggt aggcgtagaa ggcccgtcgg gcggggtcca
    14761 tcgacgcgtg gctctcccag gcctcgggga tcatcatgag gacggcgtgc ggcagcgatc
    14821 ggccactgag gtgcagcagc tcgagcacct cgtcgaagga cgccgagtca gatgcacccg
    14881 gggtgcagat cgggaagagc cgctgcaagt cgccggggat gaggtcggtg gcgagcatcg
    14941 actcgcgggc ccgcatccag ttgcggttgc ccttgaccgt gttgatctcg ccgttgtggg
    15001 cgatcagccg gtaagggtgg gccaacggcc aggaggggaa ggtgttggtg gagaaccgcg
    15061 agtgcaccag ggcgagctcg gtctcgaacc gggggtcgga caggtcgggg aagaacggct
    15121 ccaactgccc ggtcgtcagc atgcccttgt agaccagggt gcgcgcggac agcgacggga
    15181 agtagacccc gacctcgcgc tcggcccgct tgcgcaggca gaaggcggcc cggtccaact
    15241 ccagcccggt gcggccttgg gtcgactcga cgaagacctg ggcgaagaac ggcatgacga
    15301 ctcgggcggc gtcgccgacc aggccggggg tgacgggcag gtcacgccag ccgatgaccc
    15361 gcaggtcctc ctgcgtggcg atgttctcga tgcgctcgat cgcgtcggca cgctcggccc
    15421 cgttggtggg caggaaggcg gtgcccacgg catacccacc gacgggcggc aacggcaggc
    15481 cggtgacctc gcgcaggaag cggtcgggca cctgggtgag gatcccggca ccgtcgccga
    15541 cgagtgggtc cgagccagtg gcgccgcggt ggtcgagatt gcgcagcgca gtcagggcca
    15601 actcgacgat gtcgtggcct gcggttcccc gcaacgtcgc caccatggcg acgccgcaag
    15661 cgtcgtgctc gaagtcacgc cggtagagcc cgacatcgtc gggttgcgcc gagaagggca
    15721 tacgcggatg cgacatagtg cctcaccgtc ctggataggc gcccacaagg gcgcggatga
    15781 cgcggctgga cggcgatggc cagcgaagga aggatagcga acccgaaaac cgtcctacag
    15841 tttgatacga ctcattccgt atgccgagac gcggggctgt tttgggcgtc tggaccgctc
    15901 acttgagcct caccagcctc accagccaga actggctcac ctggctcaac cggctccact
    15961 ggttcacggg cctcgaccgg atcgacggct cgcccggtgc gccggtagag gacgacgccc
    16021 agagcgatga cgagcagcga cacccagacg ttgacccgct gtccgaagat gcggttggca
    16081 tcgtcggtgc gcatcaactc gatgaagaag cggccgagcg gatagccgat gaccaccagg
    16141 gcgaaggcct gcccccggcg caaccggcgg tgccgctcga accacaggat gaacgcagcc
    16201 agcgcgaggc accacagcgc ctcatagagg aaggtcgggt ggaagacccc tttgaccatc
    16261 gggttgccgg ccgagtcgat cacggcgtgc ccggcctgct ggtcccattc gtagatccgc
    16321 agcccccagg gcagatcggt cggcgggccg tagatctcgt tgttgaacca gttgccgaag
    16381 cgacccagcg cctgcgcgac gaggtaaccg ggtgccacag catcggcgaa gtcaccgaag
    16441 gcgaccccgt gccgccgaca gccgatccag gcgcccaggg ccccgagggc gacggcaccc
    16501 cagatgccca ggccgccctc ccagatgcga atggcggccc acgggtttcc gccgacaccg
    16561 aagtagggct gtgggctcgt gatgaggtgg tagatgcgcc cgccgaggat gccgaacggc
    16621 acggcccacg ccgagatgtc gagcacctga ccggccgttc ctccgcgact gacgatccgt
    16681 cgctgggcga cccacacggc gacgacgatc cccaggagga tcgagagcgc atagccccga
    16741 atggggacag gcccgagcca ccacacgccg gttgacgggc tggggatctc ggtgacaagc
    16801 gtcgccaacg cccccgcact cacgccgttc ctgccttcac ggcagcccgg aacccggccg
    16861 cgtcgagccc cgacaggttc atcttcttgc cgttgagcaa cgccgtgggc gtgccgttga
    16921 cccctgaccg ctcggagttg atctccaggg cggcgatgtg agccgcgtag gtgagcccct
    16981 gctggcaggt cttccaggtg tccagcgccg acccggtgat gctcaccgac tgcgcgatgg
    17041 tcgcgagttg ggcctcggtc cagccgacgc cttcttgagc cggttgggcg gcgaaggtgg
    17101 cactcatgta ctcgcggaac ttgccctgat cggccgcgca gaaggcggcg ttggcggccc
    17161 ggaccgacga gtcgttacgc aggttgcggt cgaggaaggt catcgtgtgg acgacgacct
    17221 tggcttcgtt gttcttggcc acctcgccga tcgcggtgcc gaagatggcc tcgaactgct
    17281 ggcagatcgg gcactgaggg tcttcgtaca ggtcgagagt gggaacgccg gccggagcag
    17341 acgggttgag cacgagcggg ccgccgaagc cgacggcacc agtcggaacc ggaccggttg
    17401 cggcgggctt tgacgtggcc gtcgcgggcg ccttcgccgc gggcgcgggc gcgggcgccg
    17461 tcgctgtggc cgcgggagcg gccgggttcg cagcgggtgc tgcgccagag gcggtggtgc
    17521 cgccaccacg ggacatcgac tgaccaagca ggaccgccac gacgatcgcc agcacggcga
    17581 cggccgcgac gagcacgccc atcacgccgc gacgagagct cgcgggtgga ggtgtcgcta
    17641 cgggcgcagc gccggccacg ttggctgcac cggcgaacgg atccgcgggg aactcgggtg
    17701 gttgcccgac agggtcctgt aacgacatgc cgcccattct ccctcatccg ggagggcgcc
    17761 ccggcccagg cccgggcggg gctggtgcgg cgcagactac ttcgtcgcgg ccttgacggc
    17821 ttccgcaagc tcgttgacgt tgccggtgag cgtcagtttg ttgccgttga ggcgcagcgt
    17881 gggggtgctg ttcacgccct ccttgagggc ggcctcggcg gctgctgcgg tgtgtgcctg
    17941 gtaggtcttg tccttgacgc actgctgcca ggtggtcagg ttgcccaccc gggccgacat
    18001 ggcgaacgcc tccaactgcg gggcggtcca gcccacccct tcctgctggg gctgaccaac
    18061 gtagacagcc gacatgtagt ccaggaactt gccctggtca gccgcgcagt aggcgcccat
    18121 cgcggcccgc accgacgcat cgttcttgag gttggtgtcc aggaacgtca tcgtgtggat
    18181 gacgaccttg gcctggttgg acttggccag ctcagcgatc gccgcgccgt agaccttgtc
    18241 catcgtcgcg cacgcggggc actgagggtc ctcgaagacg tccagcaccg gcaccgaagc
    18301 gggagcctcg gggttgacga ccatgggcgc acccagcgac gcggcgccct tggggaaggc
    18361 agtcccgcct ccgccgctgg acttggacga ggtcgtggca ccgatgacga cggcgacgac
    18421 cacggccacg aagacgacgg cggcaatgat gatcgccagg ccactacggg agcgacgggg
    18481 agcggtggcg gcgagcttgg cagaggcgtc tgggcgcggc atacctccca ttgtctctca
    18541 tgtcaggcgc gcgctcgccg ggtgcggtca gatccggtcg tcgagggcgt atgccgtgtg
    18601 cggacgccgc accagccacg cccccgccag cagcagggcg acgtcgcgac tcaactcgag
    18661 cggataggcg gtctgttccg cgccgatggt gccgccgccg ccgaagcagc cgcagtcgat
    18721 cgacaggcct cgcgcccagg ccgacgcgat cccgatgacg aacgccagca tgagcaaggc
    18781 gcccaaaagg gcgctcatcc gggtaaacag cccgaggacg agcaggagcc cgatgacgat
    18841 ctcgatgatc ggcagcgcat agccgacata acccgcgagg tcgtagggca gcagctggta
    18901 ggcccgcacg gcgagcgcgg actgcccgag gttggtcacc ttgagcgccc cggcgacgag
    18961 gatcacgccg ccgaggacga gccgcaggag ggttcccagc aggtccagcc cgcgctcgcg
    19021 agtcacggcc ggccctcgcg gaccccggcc gccaactcgc gggtgagggc ttccatcgcc
    19081 ggtatgccgc cctcccccaa ggcgcgcacg agcgccgagc cgacgatgac cccgtcggcg
    19141 aaccccgcga cgtccgcggc ctgggcccgg gtcgacacgc cgagcccgac acacacaggg
    19201 acgtcggtca cctcgcgggt gcgtcgcacg acgtcctgcg cggccgagcc gaccgtctcg
    19261 cgggtgccgg tgacacccat cgtcgacgcg gcgtagacga agccccggca ggcccccgtg
    19321 gcgaccgtga gtcgctcggg ggtggacgac ggtgcgacga ggaagacccg gtccagaccg
    19381 tgggcatccg aggcggcgag ccactcgccg gcctcgtcgg ggatgaggtc aggggtgatc
    19441 aggcccgagc cacccgccgc ggcgaggtcg gcggcgaacc gcgcgacccc tcgcttgagc
    19501 acgaggttcc agtaggtcat cacgtatgcc gccgcgccgg cgtccgcgat cgcccggacc
    19561 gcagtgaaca cgtcatcgac ccgcgagccg ccctcgagcg ccgcttccgt agcccgttgg
    19621 atcagcggac cgtccatgag tgggtcggag tagggaatgc cgacctcgat gatgtcggcg
    19681 ccggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgtcc atgagtgggt
    19801 cggagtaggg aatgccgacc tcgatgatgt cggcgccggc tcgaaccatc gccaccatcg
    19861 cctcgatcga gacctccacc gacgggaagc cgaccggaag gtagccgatg agcgctgcgc
    19921 gaccctcggc ccggcaagct gcgaggacct gggtgccggg gctcagcgcc gggctggcgg
    19981 gattcgcggc gagagggctc gcaggggtga ggttcacggc ataccctccg cgtcgtcgag
    20041 cagcccgaac cagcgagccg cggtgtgcac gtccttgtcg ccgcgcccgg agagcgagac
    20101 gagcagcagc gcgtctggcc ccaactcacg cccgaccttc atcgcacccg cgagggcgtg
    20161 cgacgactcg attgccggga tgatcccctc ggtgcgacac agcagccgga acgcctccat
    20221 cgcctcgtca tcggtgatcg gccgatattc ggcgcgtccc gtctcgaaga ggtgggcatg
    20281 ctcagggccg acagaggggt agtccaatcc ggctgacacc gaatgggatt cgatggtctg
    20341 cccgtcgtcg tcctgcagca cgtaggtggc cgcaccatgc aggacccctc gggtgccgcc
    20401 ggagaagcgc gctgcgtgac gaccgctctc gatgccgtcg ccgccggcct cgaagccgat
    20461 cagccgcacc gagtcgtcga agaggaaccg gtggaagatg cccatggcgt tggagccacc
    20521 gccgacgcac gcgaggacgg cgtcgggcag tcgaccgacg aggtcgagca cctgggcccg
    20581 ggcctcgacc ccgatgatcc ggtggaagtc acgcaccatc tcggggaacg ggtgcggccc
    20641 agtcaccgtg cccagcacgt agtgggtcga gtcgacgttg gccacccagt cgcgcagggc
    20701 ctcgttgacg gcgtccttga gggtctggct gccgttggtc accgcgacga cctcggcgcc
    20761 gagcaagcgc atccgggcga cgttgagcgc ctggcgcttc gtgtcgaccg cacccatata
    20821 gacgacgcag tccaggccca gcagcgccgc ggcggtcgcc gtggccaccc cgtgctgtcc
    20881 cgcgccggtc tcagcgatga cccgggactt gcccatccgc ttggccagca acgcctggcc
    20941 gaggacgttg ttgatcttgt gcgagccggt gtggttgagg tcttcacgct tgaggatgac
    21001 ccgcgccccc ccgcactgct ccgcgaagcg cggcacctcg gtgatgatcg acgggcgccc
    21061 ggtgtaggtg cggtgcagcc ggtcgagttc gccgatgaac tcggggtcga cggctgcctt
    21121 gcggcgcacc tcgtcgagtt gttccagggc ggggatgagc gcctcgggga cataccgtcc
    21181 cccgaagtcg ccgaatctcc cgtgcggtgt ggtcatctcg gtccctccgt cgtgctcgcc
    21241 tgcgtcgtgc tcgcctgcgg ctgtcccggt atgccgccca ccggcacccc cgcgaaactc
    21301 gccaccgcgc cggccgggtc gccgccgatc accagtgcct caccgacgag caccgctcgg
    21361 gcgccctcgg cggcataccc cttcacgtcg gcgacgccgc ggatgccgga ctcggcgacc
    21421 gcaaccgcct cgtcggggat gagcgggcgc agccgggcga aggcggacgg gtcgacctcg
    21481 agggtcttga ggttgcgcgc gttgaccccg atgacgcgag ccccggtcgc gacagcccgg
    21541 cgggtctcct cggcgtcgtg cacctcgacc agcgccgtca tccccagccg ctgcgcctgc
    21601 tcgcgcatag cgatcagttg aatgtcagag agcgcggcga cgatgagcag gatgaggtcg
    21661 gcgccgtggg cccgcgcctc gaggagttga tacggctcca ccatgaagtc cttgcgcagc
    21721 acggggatcc gcacggtggc gcgcaccgcg tcaaggtcgg ccagcgaccc ggagaagcgt
    21781 cgctcctcag tgaggacgga gatcgcggtg gctccgccag cggcatacgc agtcgccaag
    21841 gccgccgggt cggggatgtc cgcgagggcg cccttgctgg ggctggcccg cttgacctca
    21901 gcgatcacgg cgatgccggg agcggcccgc agggcggcct ctgcgtcgag ggcgggaggc
    21961 atcgtcgcga tccgggcctc gaggtcagcc aggctcgtgc gggcgacgcg gtcagcgaga
    22021 tcgaggcgga cgccagcaat gatctcgtcg agaacggtgg gtgtggcagg caccgaccca
    22081 ggctacgtgg tcaggctcag agcggggaca accatgtcat ccctgggacg ttgcgcagga
    22141 tccagaacgc gacgatcgac acccacacga cggtgatgac ccacccgggc gcgagtcgca
    22201 gcggggtgcc ggtgacccgg cggtaggccc agcggtagaa cgtccaagcg atgtagatcg
    22261 aggcgagcac cgcgaagggg ttgcgggcca gagcggtgcc gacgtcgagg tgcagcaggg
    22321 cgtgcagtgc ccgtaaggag ccacaaccgg ggcaccagag gccggtgacg gccaggaacg
    22381 ggcacggcgg atagtggccg ggggcgttgg ggtccacggc atacaggtat gccgcccccg
    22441 ccgccgcgcc tacggcgatc agggcaggtt gcacaaggcc gcgcaccccc atgccaacac
    22501 ggggggacgc ggcctcggcg atcgtcacaa ggacggttgt cagcggaccg tcacactgcc
    22561 gtttgcggcc gcgaggaaga cgaacacgaa gtagatgatg ccgacaacga tgccgaggat
    22621 ggccgagatg atcgaccact tcttcgcctc tgctgcagcg gcgtgggcgc cggcctggtc
    22681 gccctgggcc cacagggtgt tgaccttgtt ggccttgagg atcgccacga tgcccagagg
    22741 caggcagcac aggacggtcg cgaggatggc caggacgagg ttgttctgcg ggggcgggct
    22801 catcggagcg cccccatagg gcatccctcc ggcaggaccc atgggcggct gagcgccgta
    22861 gccaccagaa cccggcggcg ggggcggcgg agtggaaccg taggacatga cactgcctct
    22921 ttctcgatca cgacaacaac gcgccccccg gacgaggccg cgacaaagac cctagcgttc
    22981 accgcgcacg gtggccaacg aacccgccga atcgagatgc cccgcggacc ggcgcatcga
    23041 gggacggccc tgggtcagga aacgccgctg tccacctggt tgcggcccgg cttctggacg
    23101 ccgaaaccgg ccatggccaa cacctttccg gtgacgacgc cgatgacggc aagcacggca
    23161 ccggcgatga acaacccggt cgaggcaacg acgacggccc aacacatgat ggctgcggcc
    23221 accgtgagga tggccactcc ggtccacgcg gcaacgctct gaccgtggtt gtcgtggtgg
    23281 tcgtttccgg ccatcagtgt cactccttct acggccgccg gggtcggcgg gcacccgtca
    23341 cggatttcct gtgcgccatt gtgtcaggtc ccctggccac tgtccgcgcg ggtggggtcg
    23401 tgtccttcgg tgaggtcgtc ccagtcgctg cgccgagccc cacgatcggt cgcgccgtcg
    23461 acgcctccgc gatcgaaccg tgcggacagg ccggcccatc gacgcgccga gaaggtcgcc
    23521 acgatcgagg ccacggcggt gagtccggcg cccgtggcgg cgatccacgc ccacggcccg
    23581 agcgcgctcg tcaccgggac cggggaaccc gtgcggccgg ccaactccgc ggcccgccga
    23641 cccagggcgg ccccagccga gccgagcgag gccccgatga gccccacgac agcgagcgca
    23701 gccagcgcca tgaggacccc ggccgcggtc cggatccgcg gacctccggt gagcatcgcg
    23761 accagcgctg caccgaccac gagggccaga cccagcgccg cggggaccat ctgcgtgccc
    23821 gtaatgctca gggccgcccg gcccagcacc gggtcctggc tggacccact ggcccacacc
    23881 tggcccagcg ccgccagcag caccgccaac gggccgccga cggcgaggac gaccaggcgt
    23941 ttggtcacgg ggagaccgcc accgctgcct gcatccctgc ggcggcctgg acggcccgca
    24001 gcacggcggc agccttgtgc tggcactcct cgtgctccag ttggggcacg gagtcggcga
    24061 cgatccccgc acccgcctgg acgtgtgcca cgccgttgcg gatgaccgcg gtgcggattg
    24121 cgatcgccat gtcgaggtca ccggcgaagt cgagataacc caccactccc ccgtagatcc
    24181 cccgcttgag cccttcgtac tcgtcgatga gggccattgc acgtggcttg ggtgctcccg
    24241 acagggtgcc cgcagggaag gtcgccacga gcacgtcgta tgccgccacg ccctccttga
    24301 gctcgccgac gaccgtggac tccagatgca tgatgtggct gtagcggcgg accgtcatga
    24361 actcgaccgt gtcgacggtg cccgcgcggc atacccgctg gaggtcgttg cgcgagaggt
    24421 cgacgagcat gacgtgctcg gcgcgttcct tggtgtcggc ggcaagatcg tcggccaggc
    24481 ggacgtcgtc ctcgggggtc ttgccacggg ggcgcgaacc ggcgatcggg tgggtgatcg
    24541 cctcgcgccc ggtcaccttg atgagggcct ccgggctgga ccccacgatg tcgtatgccg
    24601 tgccgtccgg gtgcggcagg cgcagcaggt acatgtaggg gctggggttg gcggcccgca
    24661 gtgcccggta gacgtcgatg gggtcggcgt cgcacggcgt cgagaaccgt tgcgagacaa
    24721 cgatctggaa ggcatcgccg gcgcggatcg actccttgca ccgctcgacg ttgtcgaggt
    24781 aggcctccgg ctcgtgggaa ctggtgacgg tgagggtcac cggctcgatc acagcgaccg
    24841 tcgagggcgc cggctcaccg agttcggcgg tcatccggtc gagccgagcc accgcatcac
    24901 gccaggccca ctccatccgc tcgtcagtgt cgtcgtagtt gatcgcgttg gcgacgagca
    24961 gcaccgagcc gtcggagtgg tcgaggaccg cgagatcggt ggccagcacc atggccagtt
    25021 cggggaggtt caactcgtcg cggccggtgt cgggcacctt ctcccaccgc cggaccgcgt
    25081 cgtaggtgat ggccccgacc atcccgccgg tgagcggggg cagaccgccg atcctcgcgg
    25141 tcgcgagggc ggcgacggtg tcgcgcaagg cgatcgtcgg gtcgccgtcg gtcgggaccc
    25201 cggcgggcgg cataccgatc cagtgagcct ggccgtcccg ctcggtgagg gtcgccgcgc
    25261 tgtgggcccc gacgatcgag tagcgcgacc agaccccacc gtgctcggcc gactcgagca
    25321 ggaacgtgcc cggctcgccc ttggccagct tgcggtagac cccgagcggc gtctcaccgt
    25381 cggccagcag ccgccgcacg acggggatga cccgacgggt gcgagccaac tctccgaaga
    25441 cgtccaggct cggccaggtg ctgccgaagg tcaactcggc gtggatgtca ctcatcgtgg
    25501 cctcccacca cggccagcgg ccgcgcgtcg aagcaggtgc ggtcgccggt gtggcaggcc
    25561 gcgcccacct ggtcgacgcg cacaagcagg gcgtcgccgt cgcagtccac ggccacggaa
    25621 cggacgtgtt ggacgtgccc ggaggtgtcg cccttgcgcc agtactcacc gcgcgagcgc
    25681 gaccagaagg tcacccggcc cgtcgtcagc gtgcgagcga gggcctcgtc atccatccac
    25741 ccgagcatga gcacctcacc ggtgtcgtgc tgctggacga tggctgccac gaggccctgg
    25801 tcgtcgcgct tgagcagggc ggcgacggcc gggtcgaggg agctggcgcg aagggacacg
    25861 gctgccattc tgcccgctcc gctggggcgg tggcggcggc gtcccatggc ggcggagcga
    25921 tgggtgcagt ggaacgcggc gcaggaacct cgttcggcca caccctggtc ggggtcgatg
    25981 tgccctagcc ttgggcgccg tgagggaggc gttcgtcgac tttccctggg ccccggccgg
    26041 ggctgcgggg ccgctggcgt tcggcgcgcc cgtcgagatc gtcgaggcgc gctgcctcga
    26101 cgaggtccga cccgcgctgg atcgggtcgc ggcagcgtgc gcggacggtc ggtatgccgt
    26161 cgggttcgtg tcgtatgagg ccgcccctgc gttcgatccg gcgatggcgg tccgcagcgg
    26221 gtccggcgtg cccctcgtct ggttcggcct gcacgacgct cccctcgccg agggggcggc
    26281 acgggagccc gcggcatacg cgtgctcggt gtgggaggcg ggcaccgacg aggtgacgta
    26341 tgccgcgtcc gtcgccgccg tccgcgaggc catcgccagt ggtgacacct atcaggtgaa
    26401 ccacacgatg cggctgcggg cgcgcttcga cggggatccg ctgggggcct gggcggacct
    26461 gcgccgggcg cagcgcgacg ggtggttcgc ctacgtcgac acgggtgccc atgcggtcgc
    26521 ttcggtgtcc cccgaactgt tcttcacctg ggacggccac gaggtggtca cccgcccgat
    26581 gaagggcacc cgcgcgcgat ctcgcgacgg ggcgctggac gccgcccgag ccgcggacct
    26641 gcgcgcctcc gccaaggacc gggccgagaa cctcatgatc gtcgacctgc tgcgcaacga
    26701 cctgggacgg gtctgcaggc ccggatcggt ccgcgtgcca agccttttcg acgtcgaggc
    26761 ctatccaacc gtgtggcaga tgacctcgac ggtgaccggc gagagccgtc cggggctggg
    26821 gttgggcgac ctgtttggcg gtctcttccc ctgcggatcg atcacggggg caccgaagat
    26881 ccgcacgatg gcgctcatca cggagcttga ggatcggccg cgtggggtct attgcggcgc
    26941 ggtgggcgtg gtctttcccg gtggggcggc ggccttcaac gtcgccatcc gcacgctggt
    27001 cgtcgaccag gcggacggtc gggcgcgtta cgacgtcgga ggcggtgtcg tctgggactc
    27061 ggacggtcgc gacgagcacg ctgaggcgct ggccaaggcc gctgtgctcg acatgaccca
    27121 gcgcgacttc gcccttctgg agacgctccg ggtcgagacc gacgaggtgg ttctgctcga
    27181 tcggcacgtg gagcggatgg cgaggtcggc cgagtggttc gggtggccct tcgaccccga
    27241 ccgagcgcga gcgttggtcc gttcggacgg tgccggcccg gcccgggctc gcctgctgct
    27301 gcgcccttcg ggcgatcttc gcgtcgagtt cacctctctc acggacattt tcggtatgcc
    27361 gcccgccccc gaccgcaccg ctcccccacc tcgggcggtg gtgctggctc ggagcccggt
    27421 gtggtccggt gatccgtggt tgcggcacaa gagcacccga cgaggggtct acgagcgcca
    27481 ccggcgcgag gcgacggcat acctgcaggc ggcgggatgg gacgcagggg cgccgttcga
    27541 cgtcctgctg tggaacgagc gcggcgagct caccgagctg accatcggca acctggtggc
    27601 cgatgtcgcc gggaccttgg tgaccccgcc ggtcgcgtgc gggctgctgc ccggcacctt
    27661 gcgagctcac ctgttggcgt caggaacggt cagcgagcga gttcttcggg tgcaggagtt
    27721 gccgagcgcc acggcgctct ggctggtcaa cgccgtgcgt ggctgggtgc cgatcacgct
    27781 ggtggagccg cctgggcagc caacctgagg ccgcggcacg ctgtgcgtcg gcctgccaac
    27841 gcagcctggg gcaagggcta ccgcgaaacc cggggggaga cttcagacgg ggggacctga
    27901 gggctggttc caacgttcgc agtgttgcgc cgaatcgcgc gt
//
LOCUS       DAORPW010000014        27406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_259_length_27406_cov_748.462616, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000014 DAORPW010000000
VERSION     DAORPW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 27406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27406
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_259_length_27406_cov_748.462616"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1047)
                     /locus_tag="PLB86_02430"
     CDS             complement(4..1047)
                     /locus_tag="PLB86_02430"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRC64022.1"
                     /translation="MPSAAVNVGIVGATGQVGGVVLRILAERNFPVGQLRLFASSRSA
                     GSTITWQGRDIVVEDASTADPTGLHIAIFSAGATTSKAQAPRFAAAGVTVIDNSSAWR
                     MDPEVPLVVSEVNPAAAKNPPKGIIANPNCTTMAAMPVLKPLADEAGLKRLIVSTYQA
                     VSGTGLAGVQELATQVRRALESGDIEGLAHDGRAVDLGAPVKYLAPIAFDVVPVAGSV
                     LDDGSEETDEERKLRNESRKILDLPDLRVSGTCVRVPVFTGHSLSINAEFERPITPAR
                     ARELLANAPGVELADVPTPLQAAGADPSYVGRIREDQSVDGGRGLALFVSNDNLRKGA
                     ALNTVQIAELLIS"
     gene            complement(1050..2324)
                     /locus_tag="PLB86_02435"
     CDS             complement(1050..2324)
                     /locus_tag="PLB86_02435"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRC64023.1"
                     /translation="MSVIVQKYGGSSVADAEGVKRVARRIVETKQAGHDVCVVVSAMG
                     DSTDELIDLANKVTPNPPARELDMLLTAGERISMAVLAMAIADLGSEARSFTGSQAGV
                     ITDAEHGKARIIDVTPGRITEALAAGHIAIVAGFQGVSQTSKDITTLGRGGSDTTAVA
                     LAAALKADVCEIYTDVDGVFTADPRVLPTARKLSVISSEEMLDLAANGSKILHLRCVE
                     YARRHGIAIHVRSSWSGHEGTWILENPYGEGSTMEAPIIAGVAHDRSEAKITIVGVPD
                     EAGKAADIFTAVADAGINIDMIVQNVSAAATGRTDISFTLPSSDGPKAVQTLQSLRAA
                     IGFDELRFDDQIGKLSLVGAGMRTNPGISAKLFRALAHAGINIEMISTSEIRISVVTR
                     ADQLDAAVRAAHTAFDLDSAEGEAIVYGGTGR"
     gene            complement(2429..3019)
                     /locus_tag="PLB86_02440"
     CDS             complement(2429..3019)
                     /locus_tag="PLB86_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HRC64024.1"
                     /translation="MSASAEPGSEFDDFAEFAGLADDSAVQARAYVTAVTEVASGSSP
                     DIAIPLLLLATAQLLLAGSRLGAVQDVVLDERYEPDPGPDADVEPLRDNLANLFEGID
                     DYADLVDPVTQAELTKGSLSNDMTEICLALAHGLSHYDAGRRVEALWWWQFSYLSTWG
                     ERAAASLRVLQSIIAHLRLDADEDVVADAEFDALHP"
     assembly_gap    3033..3132
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3215..3814)
                     /gene="recR"
                     /locus_tag="PLB86_02445"
     CDS             complement(3215..3814)
                     /gene="recR"
                     /locus_tag="PLB86_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRC64025.1"
                     /translation="MYEGIVQDLIDELGRLPGIGPKSAQRIAFHLVQADPADVARLVQ
                     VLAEVKDKIRFCELCGNVAEATLCRICGDSRRDQTAICVVEEPKDVAAIERTREFRGR
                     YHVLGGAISPMDGIGPEDLRIRELMTRLADGGIVEVIIATDPNIAGEATATYLTRTLA
                     PLGIRVTRLASGLPVGGDLEYADEVTLGRAFEGRRTVHA"
     gene            complement(3844..4797)
                     /locus_tag="PLB86_02450"
     CDS             complement(3844..4797)
                     /locus_tag="PLB86_02450"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRC64026.1"
                     /translation="MTTGDRLRGGIFGLLVGDALGVPYEFHPATQIPATDEIEMVPPA
                     GFARAHAGIAPGTWSDDGAQALVLLESLLRVGHLDVDDVGRGLVAWDDEGRYAVDRHV
                     FDIGIQTGIAIRSLRAGVPAASSGPTDSRANGNGSLMRVLPLALWHRGSDADLAADAM
                     AQSAVTHGHLRSQVCCVLYCLLARRLLQGMPPAQAWPAARAAFDEVYPAGSPEHDELV
                     THVFPDGAAYEIRGGGYVVESMRAAVALLLADDPLDYERVVKAAIALGEDTDTTACIA
                     GGLAGIVAGEGGIPLRWRTALRGRDLVEPLADALVSWRGSA"
     gene            complement(4883..5554)
                     /locus_tag="PLB86_02455"
     CDS             complement(4883..5554)
                     /locus_tag="PLB86_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC64027.1"
                     /translation="MFYRVGATLLQPVSRLLYRPTITGIENVPPKGGVILASNHLSFI
                     DSFAIPLVAPRKVSFLAKSDYFTGSGPRGLLSRGFFSSVGAIPVDRNSSRAAQESLDL
                     ALEVLQAGGAFGIYPEGTRSRDGRLYRGRTGVGWLALTAGVPVVPVGLRGTDQIQPVG
                     SRWPRLAKVSVAFGPPIHPETYAGMPAGRARRELTDDVMDAIALLSGQERADTYNEVP
                     TLDPS"
     gene            complement(5620..7800)
                     /locus_tag="PLB86_02460"
     CDS             complement(5620..7800)
                     /locus_tag="PLB86_02460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma and tau"
                     /protein_id="HRC64028.1"
                     /translation="MSTALYRRYRPETFADVIGQEHVTEPLMQALRTGRVGHAYLFSG
                     PRGCGKTTSARILARCLNCEQGPTPTPCGTCDSCVALARGGSGSVDVIEIDAASHGGV
                     DDARDLRERASYGPAQSRFKVYIIDEAHMVTPQGFNALLKIVEEPPEHVKFVFATTEP
                     EKVIGTIRSRTHHYPFRLVPPAKLTAYLEQLSAQEGVAVAPGVLGFVTRAGGGSVRDS
                     LSVLDQLIAGSGDEGVTYEGAAALLGFTSVELLDATIDALAARDAGAVFAQVDKVIES
                     GQDPRRFAMDLLERLRDLIIVAAVPDGAAQVLRGVPEDQLDRMRGQQSRFGPGTLSRA
                     ADIVNAGITEMTGATSPRLQLELICARILLPGASGESGYAARLDRIERHLDAGGGVPA
                     GPRPSMPAPPAQAPAAQAPPAQAPAAAQAPPAQAPAAAVVPEAAPVQAPVPAQAAAPV
                     PAAPAANPAAPPTSAGGFDVDAIRRVWPEVLGRIFTMRRATWTFVSEHAQVIDYDGQR
                     LVLGISTTGLTQTFRAGNHAELIRQALIDQIGLDARVEGVPMPAGGGIPSGSGGEGPS
                     AAPRGSSPAPASSQAAAPTPTPTPVAQAAPQASTPAPAPAPRSAPAPTPAPEPDIPLP
                     PEPPEAGFEPPPDPADHRPPTNHADHAHSVRAELARAKEATRRTMDERHHRSDPAPAP
                     RATSPQSDLGAASLDDEVVQEGAEIGQAVIERMLGGTVLGDTPT"
     gene            complement(7810..9234)
                     /locus_tag="PLB86_02465"
     CDS             complement(7810..9234)
                     /locus_tag="PLB86_02465"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HRC64029.1"
                     /translation="MADEPTPLTRRELREQREREEHQRAERAPESRRHADPARARRRP
                     LALGWMLHGWGIGILWLGTHLLVGAAFLLRFGANPDRWNALVVGEVYTQFAGVSQLGR
                     LPIERVLAEVPAPAVRLMQGLYAVTGEDRGRFFLAIVALAVVADLVILIALWRASAGG
                     PIAGAYWALAVPLLGPVTFARLDLVVVAVVVLAWLLAPRRPLWAGALLAVATGLGLWP
                     AAVFLPLLALVPRGSRRLAVAGYAVISLAFIAVGFLGGGWDRLASPITRALTRGLSLE
                     SIPGLPTLVDMALSPGRHTASLVNGDMVVRGPWVEALVTGSTALVVVPVVVVIALGVL
                     VLRRREALASGDARVGRAKRRAATQPTPATALVALAGLLTLLVTARTLSPETLVWTVP
                     FLAVLAARRPGRGPWLALVVLLVTQIDYPYLFQGLTYPAAAAFDVAAAAILVRNVALL
                     ALLVWSLLAAVRELRSSAGVAPGQ"
     gene            complement(9477..9573)
                     /gene="ffs"
                     /locus_tag="PLB86_02470"
     ncRNA           complement(9477..9573)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLB86_02470"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(9611..10948)
                     /locus_tag="PLB86_02475"
     CDS             complement(9611..10948)
                     /locus_tag="PLB86_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC64030.1"
                     /translation="MSDQASDGGSGASRDGGSGGTPTRPRLRDFWTDLPREGKFLLST
                     IIIDFIGTGLVMPFLVVYLHEVRGFPLDHVGVLASIPAVVSLIVLGPTGHVIDRLGAR
                     RVIIASTIASIVADVWLAFATTELAAAGALVVMGFAGGVIWPAIQSFVSAIMPSHIRQ
                     RYFGMSFTLLNLGIGIGGVIGGTVVDVARPETFVVIYLVNAATFLAPLAIYLGPLRHT
                     DPRPQHAPVDDAAPRVSYWRLLRDRRVAALFALTMVSAYVGYAQLATGAVAFARDVGQ
                     VSTQAIGYGFAANTVVIVALQLPVLQRIENRRRTRVLLAMCVVWAAAWLALGLTGVAP
                     GGAAAAVLFAVCCAVFGLGETLLQPTIPAITNDLASDAVRGRYNALMSMGFQAAAIIA
                     PIQAAWLIEHGQGGSYIGLLVAGCVVMAGLALLVERRIPASANGLARRPAPQR"
     gene            11030..11118
                     /locus_tag="PLB86_02480"
     tRNA            11030..11118
                     /locus_tag="PLB86_02480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11064..11066,aa:Ser,seq:gga)
     gene            complement(11138..12037)
                     /locus_tag="PLB86_02485"
     CDS             complement(11138..12037)
                     /locus_tag="PLB86_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HRC64031.1"
                     /translation="MKDHGPEQSEPDGSDPRVAAYLARLGRRDLLAAPADRATLVALH
                     RAHVERVPYETLQIALGHPTPVDAAASVASVLRGHGGYCFHLNGAFAWLLERLGYAVQ
                     HHRAGVQGARAVTPVGADGNHLALTVDVEGQPWLVDVGLGSAVHEPLPLAPGRYRQGP
                     FNYGIRPSEAVPGGWRFDHDPSAQSFVGMDLDIAPCTLAEATTRHTELSTSPESMFVK
                     VLAVQRRDAHGVDLLQSVALTRIDTAGRRKRVVESPADFEGVLRGVFGLDLAGMPRAH
                     LAAVWRRQADGYAAYQEKKTARA"
     gene            12266..12811
                     /locus_tag="PLB86_02490"
     CDS             12266..12811
                     /locus_tag="PLB86_02490"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64032.1"
                     /translation="MTTREPEAIDEGVVELVESLEPNPAYVAGARWDVLHANRAAHLL
                     FADFASRPDHERNMLWYYLAEPSARALYVDWEAEAIAQLGHFREDYEKWPGDPRFDDL
                     LERVFAVTPQARTWWEERRGVDLPRNGVKQIRLADGRLVSLRQLVMSTVDDPEIQVVC
                     YFADIDDDGDYGDDMESLLGD"
     gene            12837..14129
                     /locus_tag="PLB86_02495"
     CDS             12837..14129
                     /locus_tag="PLB86_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRC64033.1"
                     /translation="MSPGRAAYPSGGDHPRLARRLGLGDAVVIGLGSMIGAGVFAAFT
                     PAAAAAGSGLLVGLALAAAVAWANATSSAQLAAQFPESGGTYVYGRECLGESWGFAAG
                     WAFVVGKTASCAAMAITAATYALGGEARAAQVVGLAVVGALTVVLTRGITKTARLARV
                     LLTVTLACLLVTVLVIAAFTREWAAPWAGVGDATAYGILQSAGLLFFAFAGYARIATL
                     GEEVRAPTVTIPRAITRALGLVIALYAVLALLLLATIGADGIAASALPLLAAAQAADV
                     SWAGVFVRVGATVAALGALLGLLSGVGRTVLAMARRGDLPRLLAAVDPGSKVPAVAQL
                     WVAGAVAVLVLLTDLRHAIGFSSFGVLLYYAIANASAWTQTGEHRRWPRWVQAAGLVG
                     CLVLAATLPIGSVVVGALVVGAGVFGRVWLQRRGVPRS"
     gene            complement(14274..15875)
                     /locus_tag="PLB86_02500"
                     /pseudo
     CDS             complement(14274..15875)
                     /locus_tag="PLB86_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
     gene            15979..16275
                     /locus_tag="PLB86_02505"
     CDS             15979..16275
                     /locus_tag="PLB86_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRC64034.1"
                     /translation="MIFKGIGEHRPYPDHGLVTTRDWANVAPRQVRLQDLVTTKRTLD
                     LAQLLADDSTFYGDLFAHVVEWRGVLYLEQGLHRALRAALQQRGTLHARVLVLD"
     gene            16327..16848
                     /locus_tag="PLB86_02510"
     CDS             16327..16848
                     /locus_tag="PLB86_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HRC64035.1"
                     /translation="MSITVESASVARARRRKRTAITLLVVIAMLAGAFYYASSYWSKG
                     STKAAAPACTITAGPSGPVYPSQVTVNVYNATSRTGLAATVGRLVKAKGFVLGTVSND
                     PAKKQIPGTAEVRYGSAGERAAALVQTLVAGATLVKDTRADASVDLVLGSGYKEMATS
                     TGTATLATAPPGC"
     gene            complement(16852..17556)
                     /locus_tag="PLB86_02515"
     CDS             complement(16852..17556)
                     /locus_tag="PLB86_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC64036.1"
                     /translation="MTTPSNAPVEPGLSAEAGGLMVHSDHAEHTDHPKRSRSVTRDRL
                     LDAAGEVFAERGVAGASVEEVCERAGYTRGAFYSNFATKDELVVALLDREEGLLLERL
                     STALGASLSEPAPLTAIAGRLFDLQPFGAQNYALRAELSLLAVRDPELAAPYLAAQRA
                     CRERFVPFLLEGLAQAGLALTVAPDDALDTLDALFETSLRSAIIAGDANSRDNLVARM
                     LPVLLGAMTRPLADAD"
     gene            17677..20727
                     /locus_tag="PLB86_02520"
     CDS             17677..20727
                     /locus_tag="PLB86_02520"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRC64037.1"
                     /translation="MSSALYALGHWAVRRRRLVVALWVALIVILGASAGLLQRGMADA
                     FEIPGTQSQKAMDALSSRFPEFAGASGQVVVSAPAGSKVTDPAVTARIEDLTARYAAL
                     PHVSGATSPFNELVKGTVAPDGSAAIIAVQLDLASDDVTDATREPLIALAHEATAAGL
                     TTSVGGALFTPTAPEMSITEGLGVVVALIVLVVTLGSAIAAGIPLLTSLLGVAMSMSF
                     ILIATHFTTITSTAPFLALMIGLAVGIDYALFILSRHRDLLAEGVEVEEAAAQATGTA
                     GSAVVFAGATVAIALLALGVAQIPFLSVMGIAAAVGVGCAVVVALTLLPALIGAAGTR
                     LTPKRVADAHHARRHTSWSASWVRLVTKVPALTAILVIGGLGVAALPARDLTLALPNN
                     GTSAPGSTQREAFDTVARAFGPGANAPLVITLDIVATTDPLGVVADIEKMVLATPGIA
                     STSVATPNRGADTGVIVAIPEGSAESQVTKDALTAVRALAPEVERQFGVELAVTGHTA
                     AQVDVASRLSDALLPFGIVVVGLSLVLLMLVFRSLLVPITAAVGYLLSLGASFGAVSA
                     VAHWGWLAAPLGVSQTGPVIAFMPIIVMGVLFGLAMDYQVFLVSSMRSRYVHGDDAKD
                     AVSHGFVDAARVVTAAAIIMVAVFAAFVPKGALYIKPIALGLAVGVFVDAFVVRMTLV
                     PAVMRLLGPAAWWLPRWLDRLLPVLDVEGESLHRRIAADETRTARAAIEGPVPVIEAH
                     GLGVSDPVGTVFADVDLRVPEGGILVVHGAPGGGKTSLLLTLAGRMAFTAGSLDVAGR
                     LLPDQRHGVRKATALAEITGVNDLDPLLSIGDHLTERLAGRGWLPWVGSHARDRATGL
                     LTDLLGYAVSAVPAAHASPSLASTTRVRDLAPLERWVLGVTLALLDDPEILLVDDIDG
                     LRGSDDRAAAWAVLASLAGSPVRRTARTQPARALTVVVSCRDLHEATDALAGAGGIPL
                     VSLALASRSAEADSFVTEHTGDPWTDPVAASFPDPADELSEKVH"
     gene            20727..22832
                     /locus_tag="PLB86_02525"
     CDS             20727..22832
                     /locus_tag="PLB86_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HRC64038.1"
                     /translation="MSRPALPAAELRRFAGSPRALLALFALVLVPLVYGGLYTWANES
                     PTTRVDQIAAAVVNLDEPVTLKGADGKDQIVPLGRVVVGKLTSSASSSNYAWTLTDAA
                     TAESGLKEGRFGAVLTIPADFSRSATSTNGSPADARAARLSLRTDDAVNYLDGSIAKA
                     IATTTSAEAARTVTEGYLSAMYAGYGTLHDQLSSAADGAGKLASGVTELTSGADKAAV
                     GAGSLASGVDQIASGAGDLASGADKLATGADQAAAGVGQLAGGLSQIDSQVAAMPAGT
                     RALADGAAQVSAGLSGLSSGAGQTASGAASLATGLTAYTSGVDGLAATCAASGASATF
                     CANLSGLAAQGGGLRDGATGVSGGTAQLSGGLSQLVPGATQLAGGVDQLADGMPALAA
                     GLHQSATGAATLASKLPQLATGVRGVADGADQLASGATTAATGADELTSGLQALSDGS
                     AKLGTGASDLSTGLGEGVSKIPTYSEAERAQLAAVAATPVVADVARDNAVKNNGAGLA
                     PYFMALALWVGSLAIYLLLRPLSPRALASGAPSPLVALAGYLPGALLGAAQGAALALV
                     LQFWIGIGAASTWGLIGVGMLIGLTFVAMNQALVGLLGAPGRFVAVILAGLQLASAGG
                     TYPVATAPGFFGWLHDVLPLTYAVDALRRVVAGATDGLGTDITALVLTLVGSLLVTMY
                     AAHRQRTWTVRRLRPVTVA"
     gene            complement(23072..23161)
                     /locus_tag="PLB86_02530"
     tRNA            complement(23072..23161)
                     /locus_tag="PLB86_02530"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23125..23127),aa:Ser,seq:cga)
     gene            complement(23220..24044)
                     /locus_tag="PLB86_02535"
     CDS             complement(23220..24044)
                     /locus_tag="PLB86_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRC64039.1"
                     /translation="MSGGEWVLLGAAALLIGISKTSVGGLAAIAVAVFASVMPAKEST
                     AAVLLLLLAGDVVAVARYRRGADWGLLRRLLPSVLPGLALGAVFLWFVSDTVVRRSIG
                     GLIAVMVIVQLWQRRRGGMTAAAGGGGGGADADSASGAGGIPEGGASGTRASRAEHPL
                     AAVAAGVLAGFATMTANAAGAVMTLYLLAMRVEKVRFIGTSAWFFLIVNVSKLPFSAS
                     LGLFPAETLRLSALLAPVVLVGTWLGILLLRRLSQVTFERAALAGSVVAAVALLVR"
     gene            complement(24041..24475)
                     /locus_tag="PLB86_02540"
     CDS             complement(24041..24475)
                     /locus_tag="PLB86_02540"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRC64040.1"
                     /translation="MAHALDLARAAGQAGEVPVGAVVLDRDGGVVGVGANAREESGDP
                     TAHAEILALRAAARHTGSWRLPESTLVVTLEPCVMCAGAIVAARVRTLVIGAWDPKAG
                     ACGSVWDLVRDTRANHWVEVVGALDEEGCGEVLRAFFRERRA"
     gene            24564..25202
                     /gene="upp"
                     /locus_tag="PLB86_02545"
     CDS             24564..25202
                     /gene="upp"
                     /locus_tag="PLB86_02545"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRC64041.1"
                     /translation="MRVHVADHQLISHKLTYLRDKRTDSPTFRRLAEELVTLLAYEAT
                     RDVRVEPFSIETPVAPTVGIKLANPKPLVVPILRAGLGMLEGMMRLLPSAEVGFLGMM
                     RNEETLEAITYANRLPDDLSGRQCYVLDPMLATGGTLIDAIRYLVDRGADDITAITLI
                     SSPEGILHVEQSLADLAVPVALVTGAVDERLNEKGYIVPGLGDAGDRLYGVV"
     gene            25344..25514
                     /locus_tag="PLB86_02550"
     CDS             25344..25514
                     /locus_tag="PLB86_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64042.1"
                     /translation="MKKFWNIFRWFLLAYFIYAIVKSPDAAADIVKTLFQIIGDAFRG
                     IIGVFDSILGPK"
     gene            25522..26364
                     /locus_tag="PLB86_02555"
     CDS             25522..26364
                     /locus_tag="PLB86_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRC64043.1"
                     /translation="MGHAQRELERAQRHDGLREHLTVGEEIRLAARAHWFVLVRPFLL
                     AVVVTVLSVLILVKAPARIGSNLPVFLLAAIVLVWVRFGWRVIVRWHDMFVATDKRIL
                     KYQGIFVTDVPMMRMSKVTDMRFTRSVCGEIFGYGTIVIESAGQDQAIREVTYLPDPV
                     ENYRRLCEVIFGDKHEARGGRRRSKSWRRRLDRLTAGRRGHSANEGWDDPPFDGPPFD
                     SPPFAGSPQVLPQPAASAPDSAAPTHRPAAPAEVLFESEDIKARRRAAKTGPIPYYPT
                     NNPD"
     gene            26508..26633
                     /locus_tag="PLB86_02560"
     CDS             26508..26633
                     /locus_tag="PLB86_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64044.1"
                     /translation="MGGFIPAPLIPANGPRTSPPSGTATHEARYAVCTAYRACVG"
     gene            complement(26697..>27406)
                     /locus_tag="PLB86_02565"
     CDS             complement(26697..>27406)
                     /locus_tag="PLB86_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRC64045.1"
                     /translation="AAAAAGFMLNNDLLIITGALVGSSGAYLSYIMCRAMNRSFISVI
                     AGGFGSDGGVVGEAKDYGEHRETNAVEVADLLKNAHEVVITPGYGMATAQAQHAVADL
                     TSKLRARGVTVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDLPSTDVVLV
                     IGANDTVNPAAQDDPSSPIAGMPVLEVWKAHEVIVFKRSMNTGYAGVQNPLFFKENSA
                     MLFGDAKDRVEDIVKAL"
BASE COUNT         4050 a         9658 c         9593 g         4005 t
ORIGIN      
        1 cgatcaggag atcaacagct cggcgatctg gacggtgttg agggcggcgc ccttgcgcag
       61 gttgtcgttg ctgacgaaca gcgccaggcc tcggccaccg tcgaccgact ggtcctcgcg
      121 gatccggccg acgtaggacg ggtcggcgcc ggccgcctgc agcggggtgg gcacgtcggc
      181 caactcgaca ccgggagcgt tggccagcaa ctcgcgagcc cgcgccggcg tgatgggccg
      241 ctcgaactcg gcgttgatcg acaacgaatg gccggtgaag acggggaccc gcacgcaggt
      301 ccccgacacc cggaggtcgg gcaggtcgag gatcttgcgc gactcgttgc gcagcttgcg
      361 ctcctcgtcg gtctcctcgc tgccgtcatc gagcaccgag ccggcgaccg ggacgacgtc
      421 gaaggcgatg ggggcgaggt acttcacggg ggcgcccagg tcgaccgcgc gtccgtcgtg
      481 ggccagcccc tcgatgtccc cagactccag cgcccggcgg acctgggttg ccaactcctg
      541 aaccccggcg agaccggtgc cggagacggc ctggtaggtc gagacgatca ggcgcttgag
      601 cccggcctcg tcggccagcg gcttgagcac cggcatcgcg gccatcgtcg tgcagttggg
      661 gttggcgatg atccccttgg gggggttctt ggccgccgcc gggttgacct cggagacgac
      721 caacggcacc tcggggtcca tccgccaggc cgacgagttg tcgatgacgg tgacgccggc
      781 cgccgcgaac cgcggagcct gcgccttgga ggtcgtggca ccggcggaga agatcgcgat
      841 gtgcaggccc gtcgggtcgg cggtcgaggc gtcctcgacg acgatgtccc ggccctgcca
      901 ggtgatcgtc gagcccgccg agcgggacga ggcgaacagc cgcaactgac cgaccgggaa
      961 gttgcgctcg gcgaggatcc gcaggacgac cccgccgacc tggccggtcg ccccgacgat
     1021 tccgacgttg actgcagcgg acggcatact catcggccgg tccctccgta gacgatggcc
     1081 tcgccctccg cggagtcgag gtcgaaggcg gtgtgggcgg cccggaccgc cgcgtcgagc
     1141 tggtcggcgc gggtgaccac tgagatgcgg atctcggagg tcgagatcat ctcgatgttg
     1201 atgccggcgt gggcgagggc ccggaagagc ttcgcggaga tgccggggtt ggtgcgcatg
     1261 ccggcaccga cgagggaaag cttgccgatc tggtcgtcga accgcagttc gtcgaagccg
     1321 atcgccgcgc gcagggactg cagcgtctga accgccttgg gcccgtcgga ggacgggagc
     1381 gtgaacgaga tgtcggtgcg cccggtggcg gccgccgaaa cgttctggac gatcatgtcg
     1441 atgttgattc cggcgtcggc gaccgcggtg aagatgtcgg cggccttgcc ggcctcgtcg
     1501 ggcaccccga cgatggtgat cttggcctcg ctgcggtcgt gggcgacgcc ggcgatgatc
     1561 ggggcttcca tggtgcttcc ttcgccgtag gggttctcga ggatccaggt gccctcgtgg
     1621 ccggaccacg acgagcggac gtggatcgcg atgccgtgcc gacgggcata ttcgacacag
     1681 cgcaggtgca ggatcttgga cccgttggcg gccaggtcga gcatctcctc gctggagatg
     1741 accgacagtt tgcgcgccgt cgggagcacc cgcggatcgg cggtgaagac gccgtcgaca
     1801 tcggtgtaga tctcgcagac gtcggccttg agcgcggcgg cgagcgccac cgcggtcgtg
     1861 tccgaaccac cccggccaag ggtcgtgatg tccttgctcg tctggctgac gccctggaag
     1921 ccggcgacga tggcgatgtg cccggcggcc agcgcctcgg tgatccgccc aggggtcacg
     1981 tcgatgatcc gcgccttgcc gtgctcggcg tcggtgatga caccggcttg cgaccccgtg
     2041 aacgaccggg cctcgctgcc gaggtcggcg atggccatgg ccagaacggc catcgagatc
     2101 cgctcaccgg cggtgaggag catgtccaac tcccgagccg gagggttcgg ggtgaccttg
     2161 ttggccaggt cgatgagctc gtcagtggag tcgcccatgg cagagacgac gacacacacg
     2221 tcgtggccgg cctgtttggt ctcgacgatc cgccgggcga cccgcttgac gccctcggcg
     2281 tcggcgaccg acgacccgcc gtacttctgg acgatgacgc tcactgacct gctcccggag
     2341 acggacctgt ggaccgcccg agtttactca gggcgtgcgc aggcccggac cgtccgccgg
     2401 atcgcgagac ggccgtctca gctggcggtc aggggtgcag agcgtcgaac tcggcgtccg
     2461 cgacgacgtc ctcgtcggcg tccaggcgca ggtgggcgat gatcgattgg agcacccgca
     2521 acgatgcggc agcccgctcg ccccaggtcg acaggtagga gaactgccac caccacaggg
     2581 cctcgacgcg tcggccggcg tcgtagtggc tcaggccatg agccaacgcc aggcagatct
     2641 cggtcatgtc gttggacagc gaccccttgg tgagctcggc ctgcgtgacc gggtcgacga
     2701 ggtcggcgta gtcgtcgatg ccctcgaaga ggttggcgag gttgtcgcgc aagggttcga
     2761 catcggcgtc tgggccgggg tcgggctcgt agcgctcgtc gaggacgacg tcctggactg
     2821 cgccgagccg ggacccggcc aggagcaact gagcggtcgc gagcaacagc aaggggatcg
     2881 cgatgtcggg cgaggacccg gaggccacct cggtcaccgc cgtgacgtag gcccgcgctt
     2941 gcaccgccga gtcgtcggca agcccggcga actcggcgaa gtcgtcgaac tcggagcccg
     3001 gctcagcact ggccgacacc gcgtccggct ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nngaagtcgt cgaactcgga gcccggctca gcactggccg acaccgcgtc
     3181 cggctctgcg ggcggcatac ccgatcggtc ggcgtcaggc atggacggtg cgccgtcctt
     3241 cgaaagcgcg tcccaaggtc acctcgtcag cgtattcgag gtcgcccccc accggcaggc
     3301 ccgaggcgag gcgagtcaca cggatcccca acggcgcgag cgtgcgggtg agataggtgg
     3361 cggtcgcttc accggcgatg ttggggtcgg tggcaatgat gacctcgacg atgcccccgt
     3421 cggcgaggcg ggtcatcaac tcgcggatcc gcagatcttc cgggccgatg ccgtccatcg
     3481 ggctgatcgc gccgccgagc acgtggtagc gcccgcggaa ctcccgggtg cgctcgatgg
     3541 cggcgacgtc cttgggctcc tcgacgacgc agatcgccgt ctggtcgcgc cgggagtcgc
     3601 cacagatccg gcagagggtc gcctcggcga cgttgccgca caactcgcag aagcggatct
     3661 tgtccttgac ctcggccagc acctggacga ggcgcgccac atcggccgga tcggcctgga
     3721 cgaggtgaaa agcgatgcgc tgagcgctct tcgggccgat tccgggcagc cgtccgagct
     3781 cgtcgatgag gtcctggacg atgccttcgt acacccggcc agactacgtg cgaccggcga
     3841 caatcacgcg ctcccgcgcc aggacacgag ggcatcggcc agcggctcga ccaggtcacg
     3901 cccgcgcagg gcagtgcgcc aacgcagcgg tatgccgccc tcccccgcca cgatccccgc
     3961 cagcccaccg gcgatgcacg cggtcgtgtc cgtgtcctcg cccagtgcga tcgccgcctt
     4021 gacgacccgt tcgtagtcca gcggatcgtc ggccagcagc agcgcgaccg ccgcacgcat
     4081 cgactccacg acgtacccac caccgcggat ttcgtatgcc gcgccgtcgg ggaagacgtg
     4141 cgtcaccagt tcgtcgtgct cggggctgcc agccgggtag acctcgtcga aagcggctcg
     4201 cgcggccggc caggcctggg cgggcggcat accctgcagg aggcgccggg cgagcaggca
     4261 gtagagcacg cagcagacct ggctgcgcag atgaccgtgg gtgaccgccg actgggccat
     4321 cgcatcggcc gccaggtcgg cgtcggagcc gcggtgccag agcgcgaggg gcaggacccg
     4381 catgagcgag ccgttgccgt tggcccgcga gtcggtgggc ccggacgacg cggcgggcac
     4441 cccggcacgc agggatctga tggcgatccc ggtctggatg ccgatgtcga agacgtggcg
     4501 gtcgacggca taccggccct cgtcgtccca cgcgaccagt ccgcggccga cgtcgtccac
     4561 gtcgaggtga cccacccgca ggagcgattc gagcaggacg agggcctggg caccgtcgtc
     4621 cgaccaggtg ccgggggcga tcccggcatg ggcccgggcg aagccggcgg gcgggaccat
     4681 ctcgatctcg tcggtggcag ggatctgcgt ggctgggtgg aattcgtagg gcactccgag
     4741 cgcatcaccg acgagaagcc cgaagatccc gccgcgcagg cggtcgccag tggtcatgcc
     4801 aggcattgtc cctcgatccc ctaacacccc agtggtcccg tcggtcgggt cgatggaccc
     4861 gggcgaggcc gccgccagtc gctcaggagg ggtcgagggt ggggacctcg ttgtaggtgt
     4921 cggcgcgctc ctgaccgctg aggagcgcga tggcgtccat gacgtcgtcg gtgagctcgc
     4981 gtcgagctcg cccggccggc atccccgcgt aggtctcggg atggatcggc ggcccgaagg
     5041 ccaccgagac cttggccagc cgcggccacc tggaccccac cggctggatc tgatcggtgc
     5101 cgcgcaggcc gacgggcacg acgggtacgc cagcggtcag cgccaaccag ccgacaccgg
     5161 tgcgcccgcg atagagccga ccgtcacgcg agcgggtgcc ctcgggatag atcccgaagg
     5221 cgcctccagc ctgcaggacc tcaagcgcga ggtcgaggga ctcctgagcc gctcgcgagg
     5281 aattccggtc gacgggaatg gcgccgacgc tgctgaagaa cccgcggctg agcagacccc
     5341 gaggccccga gccggtgaag tagtcggact tggccaggaa actcaccttg cgcggcgcca
     5401 ccagcgggat ggcgaaggag tcgatgaacg acaggtggtt gctggccagg atgaccccgc
     5461 ccttgggggg gacgttctcg atgccggtga tcgtcggccg atacagcagc cgcgacaccg
     5521 gttgcagcag cgtcgcaccg actcggtaga acatgggccc aaccgtagtg gtgggcgcgc
     5581 tcgccacgca gcacccaggc caatcgatag accgcgacct caggtcggcg tgtcgccgag
     5641 caccgtgcca ccgagcatcc gctcgatgac ggcctgcccg atctcggcgc cctcttgcac
     5701 gacctcgtcg tccaggctcg ctgcgcccag gtcactctgc ggcgacgtcg cgcgcggagc
     5761 cggcgcgggg tcggagcgat gatggcgctc gtccatggtg cgccgggtgg cctccttggc
     5821 gcgcgcgagc tcggcacgca ccgagtgggc atggtcggca tggttggtcg gcggccgatg
     5881 gtcggccggg tccggcggcg gctcgaaccc cgcctccggc ggctccggag gcaacgggat
     5941 gtcgggctcc ggcgcgggtg tgggcgcggg tgcagacctg ggcgctgggg cgggcgcggg
     6001 tgtcgacgct tgcggcgccg cctgggcgac cggggtcggc gtcggggtcg gagcggcagc
     6061 ctgcgacgag gccggggccg ggctcgaacc gcggggggca gcgctcggac cttcgccgcc
     6121 cgagccactg ggtatgccgc ccccggccgg catggggacg ccctcgaccc gggcgtcgag
     6181 gccgatctgg tcaatgaggg cctgacggat caactcggcg tggttgcccg cccggaaggt
     6241 ctgggtcagg ccggtcgtcg agatcccgag gacaagccgc tggccgtcgt agtcgatgac
     6301 ctgcgcatgc tctgagacga aggtccacgt cgcccggcgc atcgtgaaga tgcggcccag
     6361 cacctcaggc cacacccgtc ggatggcgtc gacatcgaag ccgcccgcgg aggtgggtgg
     6421 cgccgccgga ttcgccgctg gggctgcagg gacgggcgct gctgcttgtg ctggcactgg
     6481 cgcttgcacc ggtgccgcct cgggcacgac ggcagcggcc ggagcctgag ccgggggtgc
     6541 ttgggcagcg gccggggctt gggcaggagg tgcttgggca gccggagcct gggcgggagg
     6601 tgctggcatc gagggccgcg gcccggcggg aacccctccc ccggcgtcga ggtgacgttc
     6661 gatgcgatcg aggcgggcgg catacccact ctcgccggac gcgccgggca ggaggatccg
     6721 cgcgcagatc aactcgagtt gcaggcgcgg gctcgtggca ccggtcatct ccgtgatgcc
     6781 ggcgttgacg atgtcggcgg cccgcgagag ggttcctggc ccgaaccggg actgctggcc
     6841 acgcatccgg tcgagttggt cctcggggac gccgcggagc acctgggcgg ccccgtcggg
     6901 gaccgccgcg acgatgatga ggtcacgcag ccgttcgagg aggtccatgg cgaaccgccg
     6961 cggatcctgg ccggactcga tgaccttgtc gacctgcgcg aagacggcgc cggcgtcgcg
     7021 cgcagccagc gcgtcgatgg tggcgtcgag caactccacg ctcgtgaagc ccagcagcgc
     7081 ggccgcacct tcgtaagtga cgccctcgtc gccggagccc gcgatcagct ggtcgaggac
     7141 cgacaacgag tcgcgcaccg acccgccgcc cgcgcgcgtg acgaacccca acactcctgg
     7201 ggcgacggcg acaccctcct gggcggacaa ctgctcgagg tatgccgtga gcttggccgg
     7261 cgggacgagc cggaacgggt agtggtgggt gcgggagcgg atcgtgccga tgaccttctc
     7321 gggctcggtc gtcgcgaaga cgaacttcac gtgctccggc ggctcctcga cgatcttcag
     7381 cagggcgttg aagccctgcg gcgtgaccat gtgggcctcg tcgatgatgt agaccttgaa
     7441 ccggctctgg gccgggccgt aggacgcccg ctcgcgcaga tcgcgcgcat cgtcgacgcc
     7501 gccgtggcta gccgcgtcga tctcgatgac gtcgaccgac ccactgccgc cccgcgcgag
     7561 tgcgacgcac gagtcgcacg tgccgcaagg cgtcggggtc ggaccctgct cgcagttgag
     7621 gcagcgcgcg aggatccggg cgctggtcgt cttgccacac ccccgcgggc cgctgaagag
     7681 gtaggcgtga ccgacccggc cggtgcgcag tgcctgcatg agcggctcgg tcacgtgctc
     7741 ctgcccgatg acgtcggcga acgtctcggg ccggtagcgg cgatagaggg cggtgctcac
     7801 ggtccgaccc tactggcccg gggcgacacc cgcgctcgac cgcagttcac gcacggcggc
     7861 gagcagcgac cagacaagta gagccagcag tgcaacgttg cggacgagga tcgcggcggc
     7921 tgcgacgtcg aaggctgcgg ccgcaggata ggtgaggccc tggaagagat acgggtagtc
     7981 gatctgggtc accaggagca cgaccaaggc caaccagggg ccgcgtcccg gccgtcgagc
     8041 ggccaggacc gcgaggaagg gcaccgtcca caccagcgtt tcggggctga gcgtccgggc
     8101 cgtgacgagc aacgtcagca gcccggcgag cgcgaccagc gcggtggccg gagtcggctg
     8161 ggtcgcggcc cggcgcttgg cccggccgac gcgggcgtca ccgctggcga gtgcctccct
     8221 gcgccgaagg acgaggaccc ccagggcgat caccacgacg accggaacca cgaccagcgc
     8281 ggtggacccg gtcacgagag cctcgaccca gggcccgcgc accaccatgt cgccgttgac
     8341 gagcgacgcg gtatgccgcc cagggctcag cgccatgtcg acgagggtcg gcaggccggg
     8401 gatcgactcc aggctcaagc cacgggtgag tgcccgggtg atgggcgagg ccaggcggtc
     8461 ccacccgccg cccaggaaac cgacggcgat gaacgccagg ctgatcacgg catacccagc
     8521 gacggccagg cgtcgagagc ctcggggaac gagcgcgagg agtggcaaga agaccgccgc
     8581 cggccacagc ccgaggccgg tggcgacggc gagcagcgcc ccggcccaga gcggacgtcg
     8641 cggggcgaga agccaggcca gcaccacgac ggcgacgacc acgaggtcaa gtcgagcgaa
     8701 ggtcaccggg ccgagcagcg gcaccgccag cgcccagtag gcgcccgcga tcggtccacc
     8761 ggcactggcc cgccaaagag cgatgaggat gacgaggtcg gcgacgaccg ccaaggcgac
     8821 gatggccagg aagaaccggc cgcggtcctc gccggtcacg gcatacaggc cctgcatcag
     8881 ccgaacagcg ggcgcgggga cctctgcgag caccctctcg atggggagcc ggccgagttg
     8941 ggacacgccg gcgaactgcg tgtagacctc ccccaccacg agggcgttcc accggtcggg
     9001 gttcgccccg aagcgcagga ggaacgccgc gccgaccagc agatgggtgc cgagccacag
     9061 gatgccgatg ccccacccgt ggagcatcca gccgagagcg aggggcctgc gccgggcccg
     9121 cgcggggtcg gcgtggcgac gggactcagg ggctcgctca gcccgctgat gttcctcgcg
     9181 ctcgcgttgt tcgcgcagtt ccctgcgggt gagtggggtg ggttcgtccg ccatgtatcg
     9241 ccttaggtga tcgaccgccc ccgcggcctt cgggtaggcc ccgaccagtc gagtatgccg
     9301 tgtgctcgcc gcaggatacc gagttgccga ctgtcgggtt ggcgcgccaa cggatgaccg
     9361 ctcattgcgg gtgagcagcg acagcactag tgcgaggcgg ccgtgtggtc gccctcgcga
     9421 gggcccgccg gcagctccgc cggcgggctc gcccatgcct ggggaacatc cgcagacgca
     9481 agagcacccc acgtacctgg aagagctcac ctgcccttgc tgcattcctg ccctggggga
     9541 gttcagtgag gtaccaccac gcggggtgcc gtcgcccagt gtatgccgtg cggcgtggcc
     9601 cgcgcgcccc tcaccgctgc ggggcggggc ggcgagccag cccattggcc gacgccggga
     9661 tgcggcgttc gacgagcaac gccaagccgg ccatgaccac gcagcccgcg acgagcaggc
     9721 cgatgtaact gccgccttga ccgtgctcga tcaaccaggc cgcctgaatg ggggcgatga
     9781 tggccgcggc ctggaagccc atcgacatca acgcgttgta gcgcccgcgg acggcgtcac
     9841 tggccaggtc gttggtgatg gccgggatcg tcggctgcag aagcgtctcg cctaggccga
     9901 acaccgcaca acacaccgcg aagagcaccg cggcagcggc gccgcccggc gcgaccccgg
     9961 tcagcccgag cgccagccag gcggccgccc agaccacgca catggccagc agcacccggg
    10021 tgcgacgacg gttctcgatg cgctgcagga ccggcagttg cagggccacg atgacgaccg
    10081 tgttggcagc gaagccgtag ccgatggcct gggtggacac ctgtcccacg tcgcgagcaa
    10141 aggccactgc acccgtggcg agttgggcat agccgacgta ggccgagacc atcgtcaggg
    10201 cgaagagcgc ggccacccgc cggtcgcgca gcagtcgcca gtaggagacg cgcggagcag
    10261 cgtcatcgac cggcgcgtgc tggggccgag ggtcggtatg ccgcaacggc ccgaggtaaa
    10321 tcgccagcgg ggcgaggaac gtggccgcgt tgacgagata gatcaccacg aaggtctcgg
    10381 ggcgagcgac gtcgaccacg gtgcccccga tgacgcctcc gatcccgatg ccgaggttga
    10441 gcagggtgaa gctcatcccg aaataccgct ggcggatgtg tgacggcatg atcgcgctga
    10501 cgaacgactg gatggcgggc cagatcaccc cgccggcgaa gcccatgacc accaacgccc
    10561 cggccgccgc cagctcggtg gtcgcgaagg ccagccagac gtcggccacg atcgaggcga
    10621 tggtcgaggc gatgatgacc cgtctcgcgc cgagtcgatc gatgacgtga cccgtggggc
    10681 cgaggacgat gaggctcacc acggcgggaa tcgaggcgag cacacccacg tgatcgagcg
    10741 ggaacccgcg cacctcgtgc aggtagacga cgaggaacgg catcaccagg ccggtgccga
    10801 tgaagtcgat gatgatcgtg ctgagcagaa acttgccctc gcgcggcagg tcggtccaga
    10861 agtcgcgaag gcgcggccgg gtcggcgtgc cgccactccc gccatcccgc gatgcgccac
    10921 tccccccgtc gctcgcctgg tcgctcatga ggcacgagtc tgacaccgac caccgacagc
    10981 cctcgaccgg atttcggccg tggcgggccg ctccggtacc ctcgcccacg gaggattcgc
    11041 atagcggcct agtgcgcacg attggaaatc gtgttgggct aaacaccctc aggggttcaa
    11101 atcccctatc ctccgccact gcggaccgcc cgtcgcctca ggcgcgggcg gtcttctttt
    11161 cctggtatgc cgcgtagccg tccgcctgcc gccgccacac cgcggccagg tgcgcacgcg
    11221 gcataccggc gaggtccagg ccgaagacgc cccgcagcac gccctcgaag tcggcggggc
    11281 tctccacgac ccgcttgcgc cgcccagccg tgtcgatccg cgtcaacgcc accgactgca
    11341 gcaggtcgac gccgtgggcg tcgcgccgct ggaccgcgag caccttgacg aacatcgact
    11401 ccggcgaggt cgacaactcg gtatgccgtg tggtcgcctc cgcgagcgtg cacggggcga
    11461 tgtcgaggtc cattccgaca aacgactggg cgctcggatc gtggtcaaac cgccacccgc
    11521 ccggcacggc ctcggagggc cggatgccgt aattgaacgg cccctgtcga taacgcccgg
    11581 gcgccaacgg cagcggctcg tgcacagcgc tgccgagacc gacatcgacg agccacggct
    11641 gcccctcgac gtcgacggtg agcgccaggt gattgccgtc agcccccacc ggggtcacag
    11701 cccgcgcccc ctggacgccc gcgcggtggt gctgcacggc atacccgagg cgctcgagca
    11761 gccaggcgaa ggcgccgttg aggtggaagc agtagccccc gtgcccgcgc aggaccgacg
    11821 cgacggacgc ggcggcgtcg acgggcgtcg ggtgaccgag ggcgatctgc agcgtctcgt
    11881 aaggcacccg ctcgacgtgg gcgcggtgca gcgcgacgag cgtggcgcga tccgcgggcg
    11941 cggccagcag atcgcgtcga ccgaggcgag ccaggtatgc cgcgaccctc gggtccgagc
    12001 cgtccggctc gctctgttcc ggcccgtgat ccttcaccgc gcaattacat caggccggtg
    12061 agttgatctc ggttacgcgt tccgtcgcgg gggcacagcg cgcggatccg ggagttgacc
    12121 aacgtgccgc aagatcgagg ccgccggtgg ccctcggatc gccggatcgg tccctggagt
    12181 cggttaccgc ttgggcgccg aagcggcctg accccccgac gagcgcggtc ggcggtcgag
    12241 gttgcccgac tggcatcatg accacatgac cacgcgggag cccgaagcca tcgatgaggg
    12301 cgtcgtcgaa cttgtcgaga gccttgagcc caaccccgcc tatgtcgcag gcgcacgctg
    12361 ggatgtgttg catgccaacc gggctgctca tctgctcttc gccgacttcg ccagccggcc
    12421 cgaccacgaa cgcaacatgc tctggtacta ccttgccgag ccgagcgccc gggccctcta
    12481 cgtcgattgg gaggccgagg cgatcgccca gctcgggcac ttccgcgagg actacgagaa
    12541 atggcccggc gacccgcgct tcgacgacct cttggagcgc gtcttcgccg tcaccccaca
    12601 ggctcggacc tggtgggagg agcgccgcgg cgtcgacctc ccccgcaacg gggtcaagca
    12661 gatccgcctg gccgacgggc gcctggtgag cctgcggcag ttggtcatga gcaccgtcga
    12721 tgaccccgag atccaggtcg tgtgctactt cgccgatatc gacgacgacg gtgattacgg
    12781 cgacgacatg gaaagcctgc tcggagactg atcgcgcccc ctggcacgat aggcgcatga
    12841 gccctgggcg cgcggcatac ccctcgggcg gcgaccatcc gcggttggcg cggcgcctgg
    12901 gcctgggaga cgcggtcgtc atcgggctcg ggtcgatgat cggcgcgggc gtgttcgccg
    12961 ccttcacccc agcggcagcc gcagccggat cgggcctgct cgtcgggttg gcccttgccg
    13021 cagcagtcgc ctgggctaac gcgacctcgt cggcgcaact ggctgcacag ttccccgagt
    13081 cgggcggcac ctatgtctat ggacgcgaat gcctcggcga gagttgggga ttcgctgccg
    13141 gctgggcctt cgtcgtcggc aagacggcct cctgcgcggc catggcgatc accgcggcga
    13201 cctatgcgct cggcggcgag gcgcgggcgg cgcaggtcgt cgggctggct gtcgtcggtg
    13261 ccctcaccgt cgtcctcacc cgaggcatca ccaagaccgc gcgcctggct cgggtgctgc
    13321 tcaccgtcac cctcgcctgc ctgctcgtca cggtgctcgt catcgccgcc ttcacccgcg
    13381 agtgggcagc gccctgggcg ggcgtgggcg acgcgacggc atacgggatc ctgcagtctg
    13441 cggggctgct gttcttcgcc ttcgcggggt atgcccggat cgccaccctc ggtgaggagg
    13501 tgcgcgcgcc gacggtgacc atcccgcgtg ccatcacccg cgcgctgggc ctggtcattg
    13561 ccctgtatgc cgtcctcgcc ctcctgctcc tggccaccat cggcgcggac gggatcgcgg
    13621 cgtcggcgct gccgctgttg gcggcagccc aggcggccga cgtgtcctgg gccggggtct
    13681 tcgtgcgggt cggcgcgacc gtggccgccc tcggcgccct cctcgggctg ctgtccggtg
    13741 tcgggcgcac ggtgttggcg atggcgcgcc gcggcgacct gccacggctg ctggcggccg
    13801 tcgaccctgg ctcgaaggtg cccgctgtcg cgcaactctg ggtggccggc gccgtggctg
    13861 tgctcgtgct gctcaccgat ctgcggcacg ccatcgggtt ctcgtcgttc ggggtgctgc
    13921 tctactacgc gatcgccaac gcgtcggcct ggactcagac gggcgagcac cgtcgctggc
    13981 cgcgctgggt ccaggcggcc gggctcgtcg ggtgtctggt gctggccgcg accctgccga
    14041 tcggttcggt ggtcgtcggc gcgctggtcg tgggtgctgg ggtgttcggt cgggtctggc
    14101 tgcagcgccg gggcgttcct cgcagctgag tgctgagttc ctcgcagctg agccctgagc
    14161 cctgagcgct gagtcctgag cgagtgtgtg acggagtgcg attgacgggg tgatcacccc
    14221 gtcaatcgca ctccgtcaag cacacgcccg cagcagcccg ccccgagcaa ctctcagcgg
    14281 cggcgagacg tgccaaagat cgaccgagtg atctcccgcc cgatgacggt gcccgccgag
    14341 cgcagcatcg acttgaacac cgacgacccg acgacctgct cgaccaccgt cggctcctca
    14401 cgagccggtc gagcagccgg gcggtcggcc ttcgcctctg ccttggcagc cgcctctgcg
    14461 gccttggcag cctctgcgtc ggccttggct tgcgcttcgg ccgccgcggc ctgggcagcg
    14521 gcggcatcgg cggccgacgc ggcagccgtg agcttctcga aggccgactc acgatcctgc
    14581 gcggcggcat accgaccggt gccgggcgac gccgcgacga tctggtcgat gaccgcgtcc
    14641 gcggagggag ccatgagcga ctgcggcgcg cgcatccggg tccaggcgac cggcgtcggg
    14701 gcacctcgct cggacagcac cgtgatgacg gcctcgccgg tgccgagttg ggtgagcaac
    14761 tcctccaggt cgtaggcgct cttggggtag gtcgacactg ccgccttgag ggccttggcg
    14821 tcgtcggggg tgtaggcgcg cagcgcgtgc tggacccggt tgccgagctg ggcgagcacg
    14881 tcgccgggga cgtccttggg cgactgcgtg acgaagaaga caccgacgcc cttggaccgg
    14941 atgagccgca ccgtctgggt gatcgcctgc aggaaatcct tcgacgcgtc ggcgaacagc
    15001 agatgcgcct cgtcgaagaa gaagacgagc ttgggcttgt cgaggtcgcc gacctcgggc
    15061 aggtcgtgga agaggtcagc caacagccac atgaggaacg tcgagaacaa ctgcggccgg
    15121 tcctgcaccg cgggcaactc gagcatcgac acgatgccgc gtccgtcggc accgaggcgc
    15181 agcaggtcgg cggtgtcgaa ctccggcagc ccgaagaaca cgtccgcgcc ctggtcggcg
    15241 aaggtgatca actcacgcag gatgacgccc gccgtcgccg acgacagccc gccgaggttc
    15301 ttgaggtcgg ccttgccctc ctcgctcgtc aggtgctgga cgacggcgcg caggtccttg
    15361 atgtcgtcga gccacaaacc cttggagtcg gcgtagtgga acaccaggcc gaggctcgac
    15421 tcctgggttt cgttgagccc cagcaccttt gccagcagcg tcggcccgaa ggaggagacc
    15481 gcggcgcgga tcgggatgcc cttgccctaa cctcccagcg catagaactc cgtcgggtat
    15541 gccgtgccca cccactcctc gccgatgtcg gctgcgcggg cggccacctt ctcgttggag
    15601 acgccgggcg acgccatgcc ggagaggtca cccttgacgt cggcgaggaa cacgggcacg
    15661 ccgccttggg acaactgctc ggccatcaac tgcagggttt tggtctttcc ggtgcccgtc
    15721 gcgcccgcca cgaggccgtg ccggttgatg acggccagcg gaatcttgac ttgggcttcg
    15781 ggataggcgg ccccatccag gacggccgcg ccgaaggcga gggcaccacc ggcgaactcg
    15841 tagccggcct tgatctgctg gatctgctcg ggcatctcgg tcacggaacg cactctagac
    15901 gtacccgcgc gcaacgaaca cgaggtggcg cgcggcatac cggaggccgc aaccgggcca
    15961 ccacctagac tggcgcccgt gatcttcaag gggataggcg agcaccggcc ctaccccgac
    16021 catgggctgg tgacgacccg cgattgggcc aacgtcgcgc cgcgccaggt gcgcctgcag
    16081 gacctcgtga ccaccaagcg caccctcgac ctggcgcagt tgctggccga cgactcgacc
    16141 ttctacggcg acttgtttgc ccatgtcgtc gagtggcgcg gcgtcctcta cctcgagcag
    16201 gggctccacc gggcgctgcg agcggccctt caacagcgtg ggactctgca tgcgcgcgtg
    16261 ctcgtgcttg actagggcac tgtctgggct gtcggggtcg cagcacttca ctgcggaggt
    16321 atgccggtga gcatcaccgt cgaatcagcg tcggtggcgc gagcgcgccg tcgcaagcga
    16381 acagcgatca cgctgctcgt cgtcattgcc atgctcgccg gtgcgttcta ctacgcctcg
    16441 agttattggt cgaagggctc caccaaagcg gcggctccgg cctgcacgat caccgcgggc
    16501 ccgagcggcc cggtctatcc gagccaggtc acggtcaacg tctacaacgc gacctcgcgc
    16561 accgggttgg ccgccaccgt gggccggttg gtgaaggcga agggattcgt cctgggcacc
    16621 gtgtcgaacg acccggccaa gaagcagatc cccggcaccg ccgaggtccg ttacggcagc
    16681 gccggtgagc gggccgcggc cctcgtgcag accctcgtcg ccggtgccac gctggtcaag
    16741 gacacccgag ccgacgcatc ggtcgatctg gtgctggggt cgggttacaa ggagatggcg
    16801 acctcgaccg gcacggcgac tctggcgacc gcaccccccg gctgctgacc ttcagtcggc
    16861 gtcggcgagg ggacgggtca tcgccccgag caacaccggc agcatccggg ccacgaggtt
    16921 gtctcgggag ttcgcatcgc ccgcgatgat cgccgagcgc aggctcgtct cgaacagggc
    16981 gtcgagggtg tccagcgcgt cgtccggagc cacggtgagc gccaaccccg cttgggccaa
    17041 cccttccagg aggaacggaa cgaaccgctc ccggcaggcg cgctgcgcag cgaggtaggg
    17101 cgcggcaagc tcagggtcgc ggaccgcgag caacgacaac tcggcgcgca gtgcgtagtt
    17161 ctgcgccccg aacggctgca ggtcgaacag gcgcccggcg attgcggtca acggggccgg
    17221 ctcggacagg gaagcaccca gcgcggtgct cagccgctcg aggagcagcc cttcctcgcg
    17281 gtccagaagg gccacgacga gctcgtcctt cgtcgcgaag ttggaataga acgctccccg
    17341 cgtgtagccc gcccgctcgc agacctcctc gacgctcgcc cctgctacgc ctcgctcggc
    17401 gaagacctcg ccggcagcgt cgagcagccg gtcgcgggtc acgctgcggc tgcgtttggg
    17461 gtggtcggtg tgctcagcgt ggtcggagtg gaccatgagc ccgccggctt cggcggacag
    17521 gccgggctca acaggggcgt tcgacggggt ggtcatctcg ttcttcaggc tagtggcgac
    17581 atcgggctga acgacagcgg atacgtcagt gtatccaata cgttggcgca tccaatacac
    17641 ttccgtattg aaccgtcgtc gctctcggga gtccggatgt cctcagcgct ctacgccctc
    17701 ggccattggg ccgtgcggcg ccgccgcctc gtcgtcgccc tctgggtggc cctcatcgtc
    17761 atcctcggcg ccagcgcggg gctgctgcag cgcgggatgg ccgacgcctt cgagatcccg
    17821 ggcacccagt cccagaaggc catggacgcc ctctcctcgc gcttccccga attcgccggg
    17881 gccagtggcc aggtcgtcgt ctcggcccca gcgggctcca aggtcaccga ccccgctgtc
    17941 accgcacgca tcgaggacct caccgcaagg tatgccgccc tcccgcacgt cagcggcgcg
    18001 acgagcccgt tcaacgaact cgtcaagggc accgtggcgc cggacggttc ggccgcgatc
    18061 atcgccgtac aactcgacct ggcctccgac gacgtcaccg atgccacccg cgagccgctc
    18121 atcgccctgg cgcacgaggc cacggcggcg ggtctgacga cgtcggtcgg cggggcgctg
    18181 ttcacgccga ccgcccccga gatgagcatc accgagggct tgggtgtcgt cgtggcgctg
    18241 atcgtcctcg tggtcacgct cgggtcggcg atcgcggccg gcataccgct gctcacctcc
    18301 ctgctcgggg tggcgatgtc gatgtcgttc atcctcatcg cgacccactt cacgacgatc
    18361 acctcgaccg cgccgttcct cgcgctgatg atcggcctcg ccgtcggcat cgactacgcg
    18421 ctgttcatcc tgtcgcggca ccgagacctg ctcgccgagg gcgtggaggt cgaggaggca
    18481 gcggcccagg ccacgggcac ggcggggtcg gccgttgtct tcgcgggcgc caccgtcgcc
    18541 atcgcgctgc tggccctcgg cgtcgcccag atcccgttcc tgtcggtcat gggcatcgct
    18601 gcggcggtcg gtgtcgggtg tgcggtcgtc gtcgccctca ccctgctccc cgcgctcatc
    18661 ggcgccgcgg gcacccggct cacccccaag cgggtcgccg acgcgcacca tgcgcggcgg
    18721 catactagtt ggtcagcctc gtgggtgcgg ctggtgacga aggtgcccgc gctgaccgcg
    18781 atcctcgtca tcggcggtct cggcgtggcc gcgctgccgg cccgcgacct caccctcgcc
    18841 ctgcccaaca acgggacgtc agcccccggg tcgacccagc gggaggcgtt cgacacggtg
    18901 gcgcgcgcct tcgggccggg tgccaacgcg ccgctcgtca tcaccctcga catcgtcgcg
    18961 acgaccgacc cactcggagt cgtcgccgac atcgagaaga tggtgctggc cacgcccggg
    19021 atcgcctcga cgagcgtcgc caccccgaac cgcggcgccg acactggcgt gatcgtcgcg
    19081 attcctgagg gcagcgccga gagccaggtg accaaggacg cgctgacggc ggtccgggcg
    19141 ctggcccccg aggtggagcg ccagttcggc gtcgagctcg cggttaccgg ccatacggct
    19201 gcccaggtcg acgtggccag ccggctgtcc gatgctctgc tgccgttcgg gatcgtcgtc
    19261 gtcgggctct cgctcgtgct gctcatgctg gtcttccggt ccctgctcgt gccgatcact
    19321 gcggccgtgg gctacctgct ctcgctcggg gcatccttcg gagcggtgag tgccgtcgcc
    19381 cactggggct ggctggccgc gccgctcgga gtgagccaga ccgggccggt catcgcgttc
    19441 atgccgatca tcgtcatggg ggtgctgttc gggctggcga tggactacca ggtcttcctc
    19501 gtctcgagca tgcgctcgcg ctacgtccac ggcgatgacg ccaaggacgc ggtgtcccat
    19561 gggttcgtcg atgccgcccg ggtcgtcact gccgcggcga tcatcatggt tgcggtgttc
    19621 gcggcgttcg tgcccaaggg tgcgctctac atcaaaccga tcgctctcgg cctggccgtc
    19681 ggcgtgttcg tcgacgcgtt cgtcgtgcgg atgaccctgg tcccggccgt catgcggctg
    19741 cttgggccag cggcctggtg gctgccccgg tggctcgacc ggctgttgcc ggtgctcgac
    19801 gtcgagggcg agagcctgca ccggcggatc gctgccgacg agactcggac ggcgcgggcg
    19861 gctatcgagg gtccggtccc cgtcatcgag gctcacgggc tgggggtgtc cgacccggtc
    19921 gggacggtct tcgccgacgt cgacctgagg gtcccggagg gcggcatcct cgtcgttcat
    19981 ggcgcgccgg gaggcggcaa gacctccctg ctgctcaccc tcgctggccg gatggccttc
    20041 accgcaggct cgctcgacgt tgccgggcgg ctgctgcccg accagcgcca cggggtgcgc
    20101 aaggccacgg ccctcgccga gatcaccggc gtcaacgacc tcgaccccct cctcagcatc
    20161 ggcgaccacc tcaccgagcg gctcgccggc cgcggctggc tgccctgggt cgggtcgcac
    20221 gcccgtgacc gagccaccgg gctgctcacc gaccttctcg ggtatgccgt gtccgccgtc
    20281 ccagccgccc acgcctcccc gtcgctcgcc tccacgacgc gggtgcgtga cctggccccc
    20341 ctggagcgct gggtcctggg cgtcaccctg gccctgctcg acgaccccga gatcctcctc
    20401 gtcgacgaca tcgacggcct gcgcggctcc gacgaccggg ccgccgcctg ggccgtgctg
    20461 gcctcgctgg ccggctcacc ggtccgccgc accgctcgca cccagccggc tcgggcgctc
    20521 accgtcgtcg tcagttgccg cgacctgcat gaggcgaccg acgcgctggc cggggcgggc
    20581 ggcatacccc tcgtgtcgtt ggccctcgcg agccgcagcg ccgaggctga ctccttcgtc
    20641 accgaacaca ccggcgaccc gtggaccgac cccgtggccg ccagcttccc cgaccccgcc
    20701 gacgagctct ccgagaaggt gcactgatgt cccgccccgc cctgcctgcc gccgagctgc
    20761 gccggttcgc cggaagcccc cgggccctgc tcgcgctctt cgcgctcgtc ctcgtcccgc
    20821 tcgtctatgg cgggctctac acgtgggcca acgagagccc gacgacgcgg gtcgaccaga
    20881 tcgccgcggc cgtcgtcaac ctcgacgagc cggtgacgct caagggcgcc gacggcaagg
    20941 accagatcgt gccgctcggg cgcgtcgtgg tcggcaagct gacgtcgtcg gcctcctcgt
    21001 cgaactacgc gtggaccctc actgacgccg cgacggccga atccgggctc aaggagggca
    21061 ggttcggcgc ggtgctgacg atcccggcgg acttctcgcg gtcggcgacg tcgaccaacg
    21121 ggtcgcccgc cgatgcgcgg gccgcacggc tgtcgttgcg caccgacgac gcggtcaact
    21181 acctcgatgg gtccatcgcg aaggcgatcg cgacgacgac cagcgccgag gccgcccgca
    21241 ccgtcaccga gggttacctc tcggcgatgt atgccggtta cggcaccttg cacgaccagc
    21301 tctcctcggc cgcagatggg gccggcaagc tggcctccgg cgtcaccgag ctgacgtcgg
    21361 gagccgacaa ggccgccgtt ggcgctgggt cgctggcgtc tggcgtcgac cagatcgctt
    21421 ctggtgcagg ggatctcgcg tccggggccg acaaactggc gaccggtgcc gatcaggccg
    21481 cggccggggt gggacagctt gcaggcgggt tgtcgcagat cgactcgcag gtcgcggcga
    21541 tgcccgccgg cactcgggcg ctggccgacg gggcagccca ggtgtcggcc ggactctcgg
    21601 ggctgtcatc cggagcgggc cagaccgctt ccggtgcggc gtccctcgcg actggcctca
    21661 cggcatacac ctcgggggtt gacgggctcg ccgccacgtg cgcggcgtct ggcgcctcgg
    21721 cgaccttctg cgcgaacctg tcgggcctcg ccgcacaggg tggcgggctg cgcgacggcg
    21781 cgaccggggt gtccggtggc accgctcagt tgtccggtgg gctcagccaa ttggttcccg
    21841 gggcaacgca gttggccggc ggcgtcgacc aactcgccga cggaatgccc gcattggcgg
    21901 cgggtttgca ccaatcggcc accggggcag cgactttggc gagcaaactc ccccaactcg
    21961 cgacgggcgt gcgcggggtg gccgacggag ccgaccaact ggcgtcggga gcgacgaccg
    22021 cggctaccgg ggccgatgag ctgacgtccg gtttgcaggc gctctccgac ggcagcgcca
    22081 agttgggcac cggtgcgagc gatctgagca ccgggctcgg cgaaggcgtg agcaagatcc
    22141 cgacctattc ggaggccgag cgggcgcaac tcgccgcggt tgctgcgacg ccggtcgtgg
    22201 ctgatgtcgc tcgggacaat gccgtcaaga acaacggcgc cggcctggct ccctacttca
    22261 tggccttggc cctgtgggtc ggctcgctcg ccatctacct gctgctgcgt ccgctgtcgc
    22321 cgcgggcgct cgcctcgggc gccccgtcgc cgctggtggc gctcgccgga tatctgccgg
    22381 gcgcgctgct cggcgccgct cagggcgctg cgctggcgtt ggtcctgcag ttctggatcg
    22441 gcatcggtgc cgcctcgacg tgggggctca tcggggtcgg gatgctcatc gggctgacct
    22501 tcgtggccat gaaccaggcc ctcgtcggcc tgctcggtgc gcccggtcgg ttcgtcgcgg
    22561 tgatccttgc cgggctgcag ttggcgtcgg cgggtggcac ctatccggtc gccaccgcac
    22621 cgggcttctt cggctggctg cacgacgtcc tgccgctcac ctacgcggtc gatgcgctcc
    22681 ggcgggtcgt cgcgggagcc accgacggtc tcggcaccga catcaccgct ctcgtcctca
    22741 ccttggtggg gtcgctgctg gtgaccatgt atgccgccca ccgccagcgc acctggaccg
    22801 tccggcgcct gcgccccgtc accgtcgcct aagccccacc gcctcgcagg ggactcatga
    22861 gttccggggg aacgtggcca agccgggcgg atgaagtcgc cgatgaagtc gcctccactg
    22921 ctgtcccagc gacagcggtg gcgacttcat gggcgacgtc atgaatcgct gctgcgccaa
    22981 cagccccaag tcgccgctca cacaccccga aacgaccaac agcccgaacc ggcgagggtt
    23041 cgggctgcta gcgatgctgc aggtccagca ctggcggtgg cgcagggatt cgaaccctgg
    23101 gtggacttcc gcccacacac gctttcgagg cgtgctcctt aggccgctcg gacacgccac
    23161 cgccgacgag gttaccggag cggacacccc gccctgaaat cgggagcctg cgccgcccgt
    23221 cagcgcacca gcagcgcgac cgccgcaacg accgaccccg cgagcgcggc ccgctcgaag
    23281 gtcacctggc tcagccgccg cagcaacagg atccccagcc acgtcccgac caacaccacc
    23341 ggcgcgagca gcgcggacag ccgcagggtc tcggccggga acagccccag cgacgccgag
    23401 aacggcagtt tcgacacgtt gacgatgagg aagaaccacg cgctcgttcc gatgaaccgc
    23461 accttctcca cccgcatcgc cagcaggtag agcgtcatca cggcccccgc ggcgttggcg
    23521 gtcatcgtcg cgaaccccgc cagcacgccc gcagccaccg ccgccagcgg gtgctcggcg
    23581 cggctcgcgc gcgtgcccga cgccccaccc tcaggtatgc cgcccgcccc actcgcgctg
    23641 tcggcatcag cgccgccgcc cccaccgccc gcagctgcgg tcatgccgcc ccggcgacgc
    23701 tgccagagtt ggacgatcac catgacggcg atcaacccgc cgatgctgcg ccgcaccacg
    23761 gtgtccgaca cgaaccacag gaacaccgcc cccagcgcca acccgggcag cacgctcggc
    23821 aacaaccgcc gcaacagccc ccaatcggcc cctcgccgat accgcgccac cgcgaccacg
    23881 tcgcccgcga gcaacaacaa caacaccgca gccgtcgact ccttggccgg catgaccgac
    23941 gcgaacaccg ccaccgcgat cgccgccaac cccccgaccg acgtcttgga gatcccgatc
    24001 aacagggccg cagcgccgag cagcacccac tccccaccgc tcacgcgcgc cgctcccgga
    24061 agaacgctcg cagcacctcg ccgcacccct cttcgtcgag cgcgccgacc acctcgaccc
    24121 agtggttggc ccgagtgtcg cgcacgaggt cccacaccga tccgcacgcg cccgccttgg
    24181 ggtcccacgc cccgatcacc aaggtccgca cccgcgcggc gacgatcgcc cccgcgcaca
    24241 tgacgcaggg ctccaaggtc accaccagcg tgctctccgg caaccgccac gacccggtat
    24301 gccgtgccgc cgctcgcagg gcgaggatct ccgcgtgggc ggtcgggtcg ccagactctt
    24361 cccgggcgtt cgcgccgacc cccaccactc cgccatcgcg gtccagcacc accgcaccga
    24421 ccgggacctc gccggcctga cccgccgctc gggcgaggtc gagtgcgtga gccagccacg
    24481 cggcataccg aggatcaggg atcggaccgg acgcaggggt gacgggcgag gagggatggg
    24541 gggtcacgcg gtaagtttcg ctcatgcgcg tccacgtcgc cgaccaccag ctcatctcgc
    24601 acaagctcac ctacctgcgc gacaagcgca ccgacagccc gacgttccgg cggttggccg
    24661 aggagctcgt cacgctgctt gcctacgagg cgacgcgtga cgtgcgggtc gagccgttct
    24721 ccatcgagac cccggtcgcg ccgaccgtcg ggatcaagct ggccaacccc aagccgctcg
    24781 tcgtgccgat cctgcgcgcg ggactgggga tgctcgaggg catgatgcgg ctgctgccgt
    24841 cggctgaggt gggcttcctc gggatgatgc gcaacgagga gacgctcgaa gcgatcacct
    24901 acgccaaccg gctgcccgat gacctgtcag ggcggcagtg ctacgtgctc gacccgatgc
    24961 tcgcgaccgg tgggacgctc atcgacgcga tccgctacct cgtggaccgg ggcgccgatg
    25021 acatcacggc gatcacgctg atcagctcgc ccgaggggat cctgcacgtc gagcagtcgc
    25081 tggccgatct ggccgtgccg gtcgccctgg tcacgggcgc ggtggacgag cggctcaacg
    25141 agaagggcta catcgtgcct gggctcggcg acgcgggcga ccggctctac ggcgtcgtct
    25201 gacgcccgtc gggatcggcg cgacgagcga gcgcctgacg tcggcgcgcc tcacgcgtca
    25261 cgtccggctc atccgtcaca ctggagcgtc atcgaggcgc cgatcgcgcg cgccccgact
    25321 caacccgatg aggagtagga cccgtgaaga agttctggaa catcttccgg tggttcctgc
    25381 tggcctactt catctatgcc atcgtcaagt cgcccgacgc cgcagccgac atcgtgaaga
    25441 cgctcttcca gatcatcggg gatgccttcc gcgggatcat cggcgtcttc gactccatcc
    25501 tgggacccaa gtagcacccc cgtgggccac gctcagcgtg agctggagcg ggcgcaacgc
    25561 cacgacgggt tgcgcgaaca cctgaccgtg ggtgaggaga tccgcctggc cgcgcgggcg
    25621 cactggttcg tcctggtgcg gccgttcctg ctcgcggtcg tcgtcaccgt gctgagcgtg
    25681 ctgatcctcg tcaaggcccc ggcccgcatc ggtagcaacc tccccgtctt tctgcttgcc
    25741 gcgatcgtgc ttgtctgggt gcggttcggc tggcgggtca tcgtgcgttg gcatgacatg
    25801 ttcgtggcca ctgacaagcg gatcctcaaa taccaaggga tcttcgtcac cgatgtcccg
    25861 atgatgcgga tgagcaaggt caccgacatg cggttcaccc gctcggtctg cggggagatc
    25921 ttcggttacg gcacgatcgt catcgagagc gccggccagg atcaggccat ccgcgaggtc
    25981 acctatctgc ccgaccccgt cgagaactac cggcgcctgt gcgaggtcat cttcggcgac
    26041 aagcacgagg cgcgtggcgg gcgacgccgg agcaagtcgt ggcgacgccg cctcgaccgg
    26101 ctcaccgccg gccggcgcgg ccactcggcc aacgaaggct gggacgaccc tcccttcgat
    26161 ggacctccct tcgacagtcc tcccttcgct ggctcgcccc aggtcctgcc gcaacccgcg
    26221 gcctcggctc ccgactcggc ggcaccaacg caccggcccg cggcccccgc cgaagtcctc
    26281 ttcgagagcg aagacatcaa ggcacgccgc cgagcggcca agaccggccc catcccgtac
    26341 tacccgacga acaaccccga ctgactcggc tgacccggct gcctggtccc cccggccatc
    26401 cccacgcctc aatgcccgga cgtcccgaca ccccgacgct gacccgggcg tgaagtcgtg
    26461 catgaagccg tcgcaggtgg cgtgatcaca ccagtaggcg cggcttcatg ggcggcttca
    26521 tccccgctcc cctgatcccc gccaacggcc cccgcacctc gcctcccagc ggcactgcaa
    26581 cgcacgaggc ccggtatgcc gtgtgcacgg cataccgggc ctgcgttggg tgaaccgacg
    26641 tggccggtgg acggaccctg cgtacccctc cgagaggcag gcgccgtcag tccgggctag
    26701 agcgccttga cgatgtcctc gacgcggtcc ttggcgtcgc cgaagagcat cgcggaattc
    26761 tccttgaaga acagcgggtt ctggacaccg gcatacccgg tgttcatcga gcgcttgaag
    26821 acgatgacct cgtgggcctt ccagacctcg aggaccggca tgccggcgat ggggctggac
    26881 gggtcgtcct gcgcggcggg gttgaccgtg tcgttggcgc cgatgaccag aacgacgtcc
    26941 gtcgagggga ggtcgtcgtt gatctcgtcc atctccagga cgatgtcgta gggcaccttg
    27001 gcctcggcga ggaggacgtt catgtgcccg gggaggcgac cggcgaccgg gtggatgccg
    27061 aagcgcaccg tgacgccgcg agcgcgcaac ttgctcgtga ggtccgcgac cgcgtgctgg
    27121 gcctgggccg tggccatccc atacccaggg gtgatgacga cctcgtgcgc gttcttgagc
    27181 aggtcggcca cctcgaccgc gttggtctcg cggtgctcgc cgtagtcctt ggcctcgccg
    27241 acgacgccac cgtcggagcc gaaccctccg gcgatgaccg agatgaacga gcggttcatc
    27301 gcgcggcaca tgatgtagga caggtaggca ccggaggagc cgacgagcgc gccggtgatg
    27361 atgagcaggt cgttgttgag catgaagccc gccgcggccg cagccc
//
LOCUS       DAORPW010000015        26751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_276_length_26751_cov_691.801506, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000015 DAORPW010000000
VERSION     DAORPW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 26751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26751
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_276_length_26751_cov_691.801506"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLB86_02570"
     CDS             <1..611
                     /locus_tag="PLB86_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRC64046.1"
                     /translation="GQVVRMSTNITYAGPGGSVEAFGVYFADAGQHLEHRLFVDHNAP
                     HCQSHVEYKGALQGDSAHTVWVGDVLIRASAEGTQTYELNRNLLLTDGARADSVPNLE
                     IETGQILGAGHASATGRFDDEQLFYLQARGIPEGEARRLVVRGFFADIINRIPVAELQ
                     GHLMQAVERELAGAGLAEVGPPDEPTLTATLTPTSDPRTADR"
     gene            608..967
                     /locus_tag="PLB86_02575"
     CDS             608..967
                     /locus_tag="PLB86_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HRC64047.1"
                     /translation="MNAELDTTFVKVCDLEDLAVGTPALADVDGIPMALVRTAPDEVH
                     AVNDVCTHANVSLSEGELDGCTLECWLHGSRFDLRTGTPSGPPATVPVAVYPVALVEG
                     AVYVSTIPAASTEEHPS"
     gene            964..1740
                     /gene="sufC"
                     /locus_tag="PLB86_02580"
     CDS             964..1740
                     /gene="sufC"
                     /locus_tag="PLB86_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRC64048.1"
                     /translation="MTVLEIRDLHVSVDVPATGGGEAEGPKEILRGVDLTVRSGETHA
                     IMGPNGSGKSTLAYSIAGHPKYHVTSGTVTLDGEDLLAMSVDERARAGLFLAMQYPVE
                     VPGVTVSNFLRTAKTAMDGQAPKLRTWVKEFRAAMDALKMDPAFAERNVNEGFSGGEK
                     KRHEVLQLEMLRPKIALLDETDSGLDVDALRVVSEGVNRAKEADDLGILLITHYTRIL
                     RYIRPDFVHVFVDGRIVEQGGPELADQLEAHGYDRFLAPA"
     gene            1762..3111
                     /locus_tag="PLB86_02585"
     CDS             1762..3111
                     /locus_tag="PLB86_02585"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HRC64049.1"
                     /translation="MTVDPFTDTESARIRADFAILSRTVRDGHPLVYLDSGATSHKPR
                     QVLDAERDFYERTNAAVHRGAHQLSEEGTDAYESARARIAAFIGAGPDEIVFTRNATE
                     AINLVAYAFSNATAGGGGRGGAGAGMPPSRTDAGTDGSPSRFVIGPGDEVLITEMEHH
                     ANLVPWQELCRRTGATLRWIPLTPDGQLDLRDLDTLLTTRTKVFAFTHVSNVLGTVNP
                     VAELARRGHAVGALVVLDACQSVPHLPVDVRELGVDLLAFSGHKMLGPLGVGVLWGRP
                     EILDEMPVFLTGGSMIETVTMEASTYAPVPQKFEAGVPVAAQAVGLAAACDYLTAIGM
                     DRVHAHETALAERLVAGLQTRPWVRILGPSDPAVRGSVVSFVVDGVHAHDVGQILDDA
                     GIAVRVGHHCAWPLHRALGVAATTRASFAAYSTAGEVDALLNALDRVPAIFGVEVPA"
     gene            3113..3613
                     /locus_tag="PLB86_02590"
     CDS             3113..3613
                     /locus_tag="PLB86_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HRC64050.1"
                     /translation="MDLYQQLILDHSKAPVRAGLRVPFDAEVHHVNPTCGDEVTLRVR
                     VADGVVEDISYAAHGCSISVASTSILAEEVTGRPVPEALDTYAAMKAMLTSRGQVPGD
                     EDVIGDGVALAGVSRYPARVKCALLGWAAFTDALARAGVDIAATSTDQSVTAAQAPSP
                     TPEPRS"
     gene            3615..3944
                     /locus_tag="PLB86_02595"
     CDS             3615..3944
                     /locus_tag="PLB86_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HRC64051.1"
                     /translation="MSTDTDTPVNVVDVEEAMRDVVDPELGINVVDLGLVYGITVDGQ
                     GQAVIDMTLTSPACPLTDVIEDQTAQALDGLVSQHRINWVWMPPWGPDKITDDGREQL
                     RALGFNL"
     gene            3952..4530
                     /locus_tag="PLB86_02600"
     CDS             3952..4530
                     /locus_tag="PLB86_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acVLRF1 family peptidyl-tRNA hydrolase"
                     /protein_id="HRC64052.1"
                     /translation="MPAGRSEVAPERFDGWLSHRLLEHPEAAVEAGDTVTTVRWDGGG
                     ARCEWFRADPLGLVLIRRGGYAVGLARGGVLQTSKAGRRHVQGRTAAGGWSQQRFARR
                     RENQADALVEAVVGHVERILVKGEGGVPDGLVLGGDRALAAAVLADPRCRALRDLPRR
                     ELPDLPDPDARVLAEALRRGRSVWIVPVPQPS"
     gene            complement(4539..5231)
                     /locus_tag="PLB86_02605"
     CDS             complement(4539..5231)
                     /locus_tag="PLB86_02605"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRC64053.1"
                     /translation="MTVPVYYVDEVTGLAGPRLYREFRQLAPLPSGTVATAVQAMLSL
                     PPSDPDYSTLWPATTLVKSVSVAAGVATVDLSAYPGTLGSGYEAAAVNQLVYTVTAAD
                     ASITGVKVRVNGSVPSSGHLDLTGIQRRGDALNQIANVWILAPTQGLSTGSPVVVKVY
                     GTGFEGNVPLKVFQGATEVASTQVTTMMGGFAEAQTSITLSPGTYELRAYNDNGRDAS
                     LQVWDTKAFTVN"
     gene            5236..5625
                     /locus_tag="PLB86_02610"
     CDS             5236..5625
                     /locus_tag="PLB86_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64054.1"
                     /translation="MAVDVEELLVLDGVVVAVAVGDGLVEVADVGADAVTVTVGDATG
                     EDPALPRMKMKATNPMITTANAAMTGASERAASGPGAPESSGGPPEGVDGSKLMGFSL
                     LDRRGAHPTARGVLAAPSVGRLPDPAG"
     gene            5703..6113
                     /locus_tag="PLB86_02615"
     CDS             5703..6113
                     /locus_tag="PLB86_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRC64055.1"
                     /translation="MSQDYRIVELSTQECWEILDRERFGRLAVAVNGDPDIFPINFLV
                     DDQKLLMRTAPGTKLTELVINSAVAVEADGRDGDTAWSVVLKGVARMVDSFSETYADD
                     GKALETWLPSEKPIYVEIIARSMSGRRFLRGSAV"
     assembly_gap    6204..6303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6318..7289)
                     /locus_tag="PLB86_02620"
     CDS             complement(6318..7289)
                     /locus_tag="PLB86_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRC64056.1"
                     /translation="MSFNENVQLDTSQIESGGGGGGGGLGRGGGGSFPGGIQVGGGIG
                     GLILLILMMIFGGGVIGGGGTQVPDTTTTGTQSSPLDQSQVSAGGQQNNDFAKCKTGA
                     DANRDDQCLVVATVNSVQAYWAQLLPQYGKQYPMTPTVLYSGSTQSACGTASNQVGPF
                     YCPLDEKIYIDASFFAQLTSQFGADGGQLAKEYVIAHEYGHRIQDILGILGKAQQDPQ
                     GPQSGSVRMELMADCLAGTWVKHATETKDANGTAFLKPITQKDIQSALSAASAVGDDR
                     IQETMQGRVSPESWTHGSSEARQKWFMAGYTSGDINQCNTFGVSRVE"
     gene            7516..9114
                     /locus_tag="PLB86_02625"
     CDS             7516..9114
                     /locus_tag="PLB86_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRC64057.1"
                     /translation="MIVATAVQLRVGARLLLGETSLRISEGDRIGLVGRNGAGKTTLT
                     KVLAGEALATSGTVTRTGAVGYLSQDPRTGDLEVLARDRILAARGLDVTLRELRAAET
                     AMASSDGAVQERAMRKYTRLDAEFNAQGGYAAESEAASIASSLGLPDRVLGQPLRTLS
                     GGQRRRVELSRILFSGAQTLLLDEPTNHLDADSIGWLRDYLKSYKGGLVIISHDVAML
                     DTVVNRVFHLDANRSEIDIYNVGWKPYLLQRETDERRRRRERLNAEKKASALLAQADK
                     MRAKATKATAAQNMARRAERLLAGIEGERAHDRVAKLRFPSPAPCGRTPLTAEGLSRS
                     YGSLEVFTDVDLAIDRGSRVVVLGLNGAGKTTLLRILAGVDAPDTGEVLPGHGLKLGY
                     YAQEHENLDVERTVLENMKTAAPDLGETEVRKVLGSFLFTGDDVDKPAKVLSGGEKTR
                     LSLAMLVVSAANVLLLDEPTNNLDPASREEILGALRTYEGAVVLVTHDEGAVDALEPE
                     RVLLLPDGVEDLWGADYKDLVSLA"
     gene            9236..9559
                     /locus_tag="PLB86_02630"
     CDS             9236..9559
                     /locus_tag="PLB86_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC64058.1"
                     /translation="MTAATTGRTPVAVARALDGLGRDLTTWRKLRHLTAEQVADRAGV
                     SRSTVLRLEGGQGASVENLLRVARALGVLELLTKALDPYATDVGRLRAEEKLPTRVRA
                     RGVSR"
     gene            9556..10818
                     /locus_tag="PLB86_02635"
     CDS             9556..10818
                     /locus_tag="PLB86_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HRC64059.1"
                     /translation="MSAVEVWVSIEGVDVRAGTLYPHRRRGGATESASFTYVDEYIAN
                     PRAYSLEPGLPLKAGAHQSTLGHPMFGAFTDCAPDRWGRTLVKRCEAAQARAAGREPR
                     SLGEVDYLLGVRDDLRQGALRFRRGDGPFEAVGDRGVPALTDLPELLTLAARAEADSA
                     DLPDLRRLIRVGSSLGGARPKAHVRGADGTLAIAKFPSAAHDTWNVMAWEKVALELAA
                     RAGITVPASTLLNLADRSVLIVDRFDRTRDADGGATGRVGYVSAMSMLRSTDGEQGSY
                     LEMAEAIEMRSSKATTDLHQLWRRIVFSILISNTDDHLRNHGFLHAGGDAWRLSPAFD
                     VNPNPEPGPKHLSTAIDLADDTASISLALSVADYFRLGAEAANDVVVHVQAVVSQWAT
                     VARQYGLSGQEVSKMSWAFEAHSARPHP"
     gene            11262..12926
                     /locus_tag="PLB86_02640"
     CDS             11262..12926
                     /locus_tag="PLB86_02640"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HRC64060.1"
                     /translation="MSTPLTTAARATGAWADGDRTPLNDNERFKLEDDGLNVRQRIET
                     IFSREGFGSIPVDDLRGRMRWWGLYTQRRPGIDGGQTAKLSADELDDSYFMLRIRSDG
                     GALSEVQLRTIAGISTEFARDTADISDRQNIQLHWIRVEDVPEIWRRIEAVGLNSAQA
                     CGDVPRVILGSPVAGIDVHEIIDGTPAIQEILDRYLLSPEFSNLPRKFKTAVSGSPSL
                     DVAHEANDVSFVGVVHPEHGPGFDVWVGGGLSTNPIFAQRLGAWVPIEDVPAVWAGVV
                     GIFRDYGYRRLRNRARLKFLVSDWGAERFREVLETEYLGRRLLDGPAPVEDPERRRDH
                     LGVHPQVDGRFWVGVAPIGGRVSGTTLTAVADLAARHGSGRVRLTAHQKLLVLDVPEA
                     EVATLVAELDALGLPANPSEWRRGVLACTGIEFCKLALVETKARARSAVEELERRIPD
                     LDAPVTIHVNGCPNSCARFQVADIGLKGLVMTGASGETVEGFQVHLGGALGGNARLAR
                     KTRALRVEADDLVDYVERVVTRFRTDQAPGESFAEWAHRADEEALR"
     gene            12923..13057
                     /locus_tag="PLB86_02645"
     CDS             12923..13057
                     /locus_tag="PLB86_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64061.1"
                     /translation="MSGTPYFCPVCGEQDLRPDETDEQSWVCRACASVFSVTIRRSGR
                     "
     gene            13054..13788
                     /locus_tag="PLB86_02650"
     CDS             13054..13788
                     /locus_tag="PLB86_02650"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HRC64062.1"
                     /translation="MSAATRSVTDLRELAHEGAALVGDGDGPAYEDRVEAARVALRWA
                     RLIFGDRLSVASSMGDEVLVHLVGRTVPGTDVFFIDTGYHFAETLGTRDAYAATGEVR
                     LRTVLPLLTVAEQAERHGDRLFERDPDACCAIRKVEPLERALGGLQAWVTGMRREDAP
                     TRTDIALVGWDARRDLVKLNPLAAWTAADVDRYTREESVMENPLRLDGYASIGCEPCT
                     RPVAEGEDPRAGRWSGRTKTECGLHQ"
     gene            13785..14759
                     /gene="cysD"
                     /locus_tag="PLB86_02655"
     CDS             13785..14759
                     /gene="cysD"
                     /locus_tag="PLB86_02655"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRC64063.1"
                     /translation="MTAVVGTSPAVETPAEASARPSVGRAAYRLDHLDHLEAEAIHVV
                     REVAGQFERPVILFSGGKDSVVMLHLAVKAFAPSPLPFALLHVDTGHNFPEVLDYRDA
                     TVARLGVRLVVASVQDYIDDGRLRERPDGTRNPLQTVPLLDAISGNRFDAVLGGGRRD
                     EEKARAKERVFSIRDAFGAWDPRRQRPELWDLYNGRHAPGEHVRVFPLSNWTELDVWR
                     YVERERIELPDLYYAHEREVFLRDGMWLTAGEWGGPRAGETPEVRTVRYRTVGDMSCT
                     GAVDSDALTVADVVLEVAGSRLTERGATRADDRLSEAAMEDRKKEGYF"
     gene            14759..16057
                     /locus_tag="PLB86_02660"
     CDS             14759..16057
                     /locus_tag="PLB86_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRC64064.1"
                     /translation="MTATIEAVPHELLRFATAGSVDDGKSTLVGRLLHDTKSVLADQL
                     AAVERVSRDRGLEATDLALLTDGLRAEREQGITIDVAYRYFSTATRSYILADTPGHVE
                     YTRNMVTGASTAELALILTDARHGILEQTRRHLAVTGLLGVRHVVLAVNKMDLVGWDP
                     SVYHRIADEFTTLAREFGISDVTAIPVSALLGDNVVDRSEHTPWYAGPTLLQHLEEVP
                     VGSDPSEQPFRLPVQLVIRPRTPAHPDYRGYAGRLTSGVVRVGDEVVIHPSGRRSRVV
                     GVDLPAVDGQVVSVAEAAAPRSVTLRLADDIDVARGDLIASPQDEPTVCRALSGPVAV
                     LSDRPLRVRDRVLARVGTRTVRGIVESIDDELDITTMTHLPTGDSLGLNAIGRVTVRL
                     AEPVALDDYRRLRHTGAFLLIDEVDGTTLAAGMAQESCLG"
     gene            16101..17201
                     /locus_tag="PLB86_02665"
     CDS             16101..17201
                     /locus_tag="PLB86_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC64065.1"
                     /translation="MSSTHISRVRRPRVRVALSLLALLATSVVSACSSSSQAASTGGA
                     STSAASTLRLGYFANLTHATAVLGVKDGHFAKALGTTTLQTQVFNAGPAAVEALLAGA
                     IDAAYVGPSPAINAYTKSGGAAIRIVAGGTSGGASFVVKPGLTETTLAGKTFASPQLG
                     GTQDVALRSWLAAHGYKTSVSGGGDVTVAPQDNSQTLSLFKAGSIDGAWLPEPWATRL
                     RLEAGATTLVDETTLWPQGRFVTTNLIVSTTFLQAHPDQVKALLQGAVAADAAIAADP
                     SGSRDKVGAAITALTSAKISPALLQEAWTHLTITPDPIASSLQTSAQAAVTVGISKSV
                     PDLKGIYDLTLLNQVLTAAGRPTVSAGGLGKE"
     gene            17204..18046
                     /locus_tag="PLB86_02670"
     CDS             17204..18046
                     /locus_tag="PLB86_02670"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC64066.1"
                     /translation="MSLTTTIDPTAEPELDPTSGAAPGSVATSPASPAVTLRGVTKTF
                     DPAQGPVIEDLSLEVAKGELVAVLGASGCGKSTLLGLVAGLDRPTSGQIVTPGGRPAL
                     MFQDASLFPWLTARGNVELALRLRRGEARRNRNDRRAEADRWLDLVRLGQHADKRPHE
                     MSGGMRQRVALARALAQGADLLLMDEPFAALDAITRDVLHEELGRIRAETGLTVLFVT
                     HNVREAVRLGDRVVLLASRPGRIVREWTVPIPHPRRIESPAVGALAVEITEQLRQEIR
                     RHAR"
     gene            18036..>18652
                     /locus_tag="PLB86_02675"
     CDS             18036..>18652
                     /locus_tag="PLB86_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRC64067.1"
                     /translation="MPADLADRTADATPAPIDETEALEAGLDVLDAPTRTTGPRARRV
                     LARVAPPVIAAAVLLLAWQVLVSSGLKPSYALPSPSAVWTSLVEQLQQGSAQRSIVGS
                     LRRAAVGFTISIILGTLLGIVLSRFRLIRRGIGPLVTGMQSLPSVAWVPAAILWFGLT
                     DAAVYAVVLLGSVPSIANGLLAGIDHAPPSYRRVARVLGAGTLAEL"
     assembly_gap    18653..18752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18753..19093
                     /locus_tag="PLB86_02680"
     CDS             <18753..19093
                     /locus_tag="PLB86_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRC64068.1"
                     /translation="PSYRRVARVLGAGTLAELRWVTLPAALPAYLAGLRQGWAFAWRS
                     LMAAELIAYSPQLGPGLGQLLDTGRELSDMPLVLASILLILFVGIGVEALIFGPVERR
                     LVRTRGLEGG"
     gene            19094..20329
                     /gene="cobA"
                     /locus_tag="PLB86_02685"
     CDS             19094..20329
                     /gene="cobA"
                     /locus_tag="PLB86_02685"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRC64069.1"
                     /translation="MGYPLTLQLRDRRVLVVGGGRVAATRVCRLLADGAHVTVVAPEV
                     GEVVAALVAEGRVTWLARGYQSTDLMQPQRAWLVHTATGDHTVDSAVADDCEGLGIWC
                     VRADDGARSAAWSPAVANGSAGTPAEGLQIAVTGDRDPRRARAVRDAILAGLESGVLP
                     VRRVRRVPGTMKPGRVYLVGGGPGDPGLLTVRGRALLAAADVVVADRLGPTDVLADLD
                     PDVLVLDVGKRPGAHPVPQEEINALLVEHASAGRTVVRLKGGDPFVLGRGGEEALHCQ
                     AHGIPVEVVPGVTSAVSVPAAAGIPVTHRGITTSFVVSSAHDGGAVAAQAPLDATLVL
                     LMGVSGIGETAARLMDAGRDPSTPVAFIERGWTPTQRTTVTTLEKAAWAVEHEQVKSP
                     AVVVVGDVVRLRETLGDLR"
     gene            20326..21036
                     /locus_tag="PLB86_02690"
     CDS             20326..21036
                     /locus_tag="PLB86_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HRC64070.1"
                     /translation="MSEREPIILLAHGSPDPRHATGIAALATRVAAAGEAAVHVAYLD
                     HHGPTPADAARAAGRGVVVPALLSLGYHARVDVPAAIEAMQAAAPGPFRAASGLGLGR
                     AVLDGVGELLVRAEVADVRDLHVVLALAGTTAPEAIEELSAALTVASAAVDGPTWAAL
                     ASVTEPTHLVGYAGPAADGVPTVVVPVALCEGVLRDRVITAALGLGLDTVAGVLAETD
                     SLARLVVARATQALLGTS"
     gene            complement(21054..21482)
                     /locus_tag="PLB86_02695"
     CDS             complement(21054..21482)
                     /locus_tag="PLB86_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC64071.1"
                     /translation="MRGRTPGSPGEDRKTRRDWWGVVLDAPDAKALGQFYADVFGWVL
                     NAEDPSFVTLDPGEGVGYLAVQTSQGYVPPTWPNEPGRQQMQLHLDIEVDNLDEAVGA
                     AVALGATQAAYQPQHDVRVLLDPAGHPFCLYCPGWPVLRA"
     gene            complement(21567..22949)
                     /locus_tag="PLB86_02700"
     CDS             complement(21567..22949)
                     /locus_tag="PLB86_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRC64072.1"
                     /translation="MSTVKRHKVILDEREMPTRWYNILHDLPTPPPPYLHPGTGQPVG
                     PEDLLALFPMDLIQQEMTTAQYVDIPPEVLDVYRLWRPSPLYRAHRLEAALGTPARIY
                     YKYEGVSPAGSHKPNTSVPQAYYNAKAGIKKLTTETGAGQWGTSLAFACAQFGLECEI
                     WQVRASFDSKPYRRLMMETFGGTVHPSPSELTAAGRAILAAHPDSPGSLGIAISEAVE
                     IAAQSESTNYALGSVLNHVLLHQTIIGEEALRQLAKIEETPDLIVGCTGGGSNFAGLS
                     FPFLREKLAGKMSPVIRAVEPASCPSLTQGTYAYDFGDTAGMTPLMKMHTLGHDFIPD
                     PIHAGGLRYHGMSPLISHIYESGLIEAVSKKQLECFAAGVLFARTEGIVPAPEPTHAL
                     AETVAEALRCKETGEEKVILTALCGHGHLDLPAYGAYLAGEMSDHSYEEAEIRDAVAE
                     ALTHLPTIPG"
     gene            23053..24249
                     /locus_tag="PLB86_02705"
     CDS             23053..24249
                     /locus_tag="PLB86_02705"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRC64073.1"
                     /translation="MVTSLPRYAARYAARRTSGVQPRGTRARRALVTLTVLTVTAVTG
                     CSAGQGDRWSALGPLSAQVGAVVTLTAVGDLLVAGRSTGAGEPALEVLADGTWTSRPV
                     QAVTPYGRVAQWLSIAAGPDGSLVAVGGARGGAHANVRWTVWRGSVAAGLTEQEQTFS
                     TFGGWGAGDQVGPVLTAAGPLLVGSWEGAQAGLDLATWVPDGEAWVREPSAGTALEST
                     PTQLMGARSVAAWGSGALITGSVVRLGDGAVRKVPAIWRSTTGNAGWTRVDLPDSGQA
                     GEAVSAACTEAECTVAGWVDGRLAVWRVGADGGARRVDGIPSVAVRDSDLIPAPAFVE
                     GHAVLLVTDHDSSRNATVVVEGRTGWSRGPGPWGTVQAFTAIGSTLYAVCQHGDTPLQ
                     VWAHKL"
     gene            24320..25111
                     /locus_tag="PLB86_02710"
     CDS             24320..25111
                     /locus_tag="PLB86_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRC64074.1"
                     /translation="MTSLTQPHPAIRVEQDGPVVTVTLCNPEHLNAQVPSMWLALADI
                     AHGLAGEVRVVVLAAEGRSFSAGLNRGMLRPGGMPGEPDLLAAAASSPEAMTELIAPF
                     QEGFAAWRRVAPVVIAAVQGHAIGAGFQLALAADLRVVADDVSFAMREVSLGMIPDLA
                     GTRPLVEAVGYSRALELCATGRFVGAEEAVRMGLATLAVPPTELSATTSDLVQALLGT
                     PPAALRALKPLLNHALTATIDEQVVRERVAQGHLLHGMARAAGLS"
     gene            25254..>26751
                     /locus_tag="PLB86_02715"
     CDS             25254..>26751
                     /locus_tag="PLB86_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC64075.1"
                     /translation="MSFGQGGPMGMMRGMAKDQSVLRARLEQGTTRRVLGYARPFVRS
                     IVAFLTIVILDAGIVVAVPLLLRDLVDLGVIPKAPDVVVRLALLVAALAVADAILSIA
                     QRWFSSRIGEGLILRLRTEVFAHVLRQPIAFFTRAQTGSLVSRLNSDVIGAQQAFTSV
                     LSSVVSNAVSLVLIVAAMATLSWQLTLAALALVPFFLIPARFMGRRLSELTRTAMTAN
                     ADLGTRMTERFNVAGALLVKLFGTPEREEREYAVRAKLVRDTGVKIAMNRTVFMVAMS
                     LVAALATAMVYGVGGLMAVSGALTVGTLLALAALLARLYGPLTALSNVRVDVMTALVS
                     FDRVFEVLDLKPLVAEAAQPVTLPAGPVAVEFDGVGFAYPSADEVSLRSLESTASGDR
                     AGGGPVLDEVSFTAYPGQLVALVGPSGGGKSTITALVARLYDPSHGTVRIGGVDLREA
                     AFGSLHETVGMVTQEAHLFHDTIRENLRYAAPDATEEQMRAALEAAQVL"
BASE COUNT         3819 a         9489 c         9144 g         4099 t
ORIGIN      
        1 gcgggcaggt cgtgcggatg tccaccaaca tcacgtatgc cggtcccggc gggtccgtcg
       61 aagccttcgg cgtctacttc gccgacgccg gccagcacct ggaacaccgg ttgttcgtcg
      121 accacaacgc tccgcactgc cagagccacg tcgaatacaa gggtgccctg caaggcgatt
      181 cggcccacac cgtgtgggtc ggcgacgtcc tcatccgggc cagcgccgaa ggcacccaga
      241 cctacgagct caaccgcaac ctcctgctca ccgacggcgc gcgcgccgac tcggtgccca
      301 acctcgagat cgagacgggt cagatcctcg gtgccgggca cgcttctgcc actgggcgat
      361 tcgacgacga gcagttgttc tacctgcagg ctcgcggcat cccggagggt gaggctcgcc
      421 gcttggtcgt ccgcggcttc ttcgcggaca tcatcaaccg gatccccgtc gctgagctgc
      481 agggccacct catgcaagcc gtcgagcggg agctcgccgg tgccggactg gccgaggtcg
      541 gcccgcccga cgaacccact ctcactgcca ccctcacccc cacgagcgac ccgaggaccg
      601 ccgaccgatg aacgccgagc tcgacaccac cttcgtcaag gtctgtgacc tggaggacct
      661 cgcggtcggc acgcccgcgc tggccgacgt ggacggcata cccatggccc tggtgcgcac
      721 cgcgcccgac gaggtgcacg ccgtcaacga cgtctgcacc cacgccaacg tctcgctctc
      781 cgagggcgag ctcgacggct gcacgctgga gtgctggctg cacgggtcgc gtttcgacct
      841 gcgcacgggc accccgtccg gaccacccgc gaccgtcccg gtcgccgtct accccgtggc
      901 gctcgtcgag ggcgccgtct atgtcagcac catccccgcc gcgtccaccg aggagcaccc
      961 atcatgaccg tcctggagat ccgcgacctg cacgtcagcg tcgatgtccc cgccacgggt
     1021 ggtggcgagg ccgaagggcc caaggagatc ctccgcggcg tcgacctcac ggtccgctcg
     1081 ggcgagaccc acgcgatcat gggccccaac ggctcgggca agtcgaccct cgcctactcg
     1141 atcgccggac acccaaagta ccacgtcacc tccggcaccg tcaccctcga cggtgaggac
     1201 ctgctggcga tgtccgtcga tgagcgggcc cgcgcagggc tgttcctggc gatgcaatac
     1261 cccgtcgagg tccccggcgt cacggtctcc aacttcctgc gcacggccaa gaccgccatg
     1321 gacggtcaag cgcccaagct gcgcacgtgg gtcaaggagt tccgcgccgc gatggacgcc
     1381 ctcaagatgg acccggcgtt cgccgagcgc aacgtcaacg agggcttctc cggaggcgag
     1441 aagaagcgcc acgaggtcct tcaactcgag atgctgcgcc ccaagatcgc gctgctcgac
     1501 gagaccgact cgggcctgga cgtcgatgcg ctgcgcgttg tgagcgaggg cgtcaaccgg
     1561 gccaaggagg ccgacgacct cggcatcctg ctcatcaccc actacacgcg gatcctgcgc
     1621 tacatccggc ccgacttcgt gcacgtcttc gtcgacggcc ggatcgtcga gcagggtggc
     1681 cccgagttgg ccgaccagct cgaggcgcac ggctacgacc gcttcctggc accggcctga
     1741 ggcggcgaac ccactcctcc catgaccgtc gatccgttca ccgacacgga gtcggcccgc
     1801 atccgggccg acttcgcgat cctctcgcgc accgtccgtg acggccaccc gctggtctac
     1861 ctcgactccg gcgcgacctc ccacaagccg cgccaggtgc tcgacgccga gcgtgacttc
     1921 tacgagcgca ccaacgcggc cgtccaccgg ggcgctcacc aactctccga agaaggcacc
     1981 gacgcctacg agtcggcgcg cgcccggatc gccgccttca tcggcgccgg gcccgacgag
     2041 atcgtcttca cccgcaacgc gaccgaggcc atcaacttgg tcgcctacgc gttctccaac
     2101 gccaccgccg gtggcggggg acgcggggga gcgggcgccg gtatgccgcc ctcccgcacc
     2161 gatgctggca ccgacgggtc tccgtcgcgg ttcgtcatcg gccccggtga cgaggtgctc
     2221 atcaccgaga tggagcacca cgccaacctc gtgccctggc aggagttgtg ccgtcgcacc
     2281 ggcgcgacgc tgcgctggat cccgttgacc cctgacgggc aactcgacct gcgcgacctc
     2341 gacacgctgc tcacgacccg caccaaggtg ttcgccttca cgcacgtctc gaacgtcctc
     2401 ggcacggtca acccggtcgc cgagctggcc cgtcgtggtc acgcggtggg tgccctcgtt
     2461 gtgctcgatg cgtgtcagtc ggtgccgcat ctgcccgtgg acgtccgcga gctgggggtc
     2521 gatctgctcg ccttctccgg ccacaagatg ctcggcccgc tcggagtcgg cgtgctctgg
     2581 ggtcgccccg agatcctcga cgagatgccc gtgttcctca ccggcggctc gatgatcgag
     2641 acggtgacca tggaggcctc gacctacgcc cccgtgccgc agaagttcga ggccggggtg
     2701 cccgtggcgg ctcaagccgt cggtctggcc gcggcctgtg actacctcac ggccatcggg
     2761 atggatcggg tgcacgccca cgagaccgct ctggccgagc gcctcgtcgc cggactgcag
     2821 acccggccct gggtccgcat cctcggcccg agcgacccgg ccgtgcgcgg ctcggtggtc
     2881 tcgttcgtcg tcgacggggt ccatgcccac gacgtcgggc agatcctcga cgacgccggc
     2941 atcgctgtgc gcgtgggtca tcactgtgcc tggccgttgc accgcgccct gggagtcgcc
     3001 gcgacaacgc gagccagctt cgcggcatac tccacggccg gggaggtcga cgctttgctg
     3061 aacgcgctcg accgcgtccc ggcgatcttc ggcgtggagg tgccggcctg acatggacct
     3121 ctaccagcaa ctcatcctcg accactccaa ggcgcccgtg cgggccggcc tgcgcgtgcc
     3181 gttcgacgcc gaggtccacc acgtcaaccc cacctgcggc gacgaagtca cccttcgggt
     3241 gcgcgtggcc gacggggtcg tcgaagacat ctcgtatgcc gcgcacggct gttcgatctc
     3301 ggtcgcctcg acctcgatcc tcgccgaaga ggtcaccggc cggcccgtcc cggaagctct
     3361 cgacacgtat gccgcgatga aggcgatgct gacctcgcgc ggccaggtgc ctggcgacga
     3421 ggacgtcatc ggggacggcg tcgccctggc cggcgtgtcg cgctaccccg cgcgggtcaa
     3481 gtgcgccctg ctggggtggg ccgcgttcac cgacgccctg gcccgggccg gagtcgacat
     3541 cgccgcgacg tcaaccgatc aatccgtcac agctgcccaa gcgccttccc ccacccccga
     3601 gcccaggagc tgacatgagc accgacaccg acaccccggt gaacgtcgtc gacgtcgagg
     3661 aggccatgcg cgacgtcgtc gaccccgagc tgggcatcaa cgtcgtcgac ctgggcctcg
     3721 tctacggcat cacggtcgac ggtcagggac aggcggtcat cgacatgacc ctcacatcgc
     3781 cggcctgccc gctcaccgac gtcatcgagg accagaccgc ccaggccctc gacggcctgg
     3841 tcagccagca ccggatcaac tgggtctgga tgccgccgtg gggtccggac aagatcaccg
     3901 acgacggccg ggagcaactg cgcgcactcg gcttcaacct ctgaggtcgc tgtgccggca
     3961 ggccgcagcg aagtcgcgcc tgagcgcttc gacggctggc tgagccatcg cctcctcgaa
     4021 cacccggagg cagcggtcga ggccggagac accgtcacca ccgtccggtg ggacggcggc
     4081 ggggcccgct gcgaatggtt ccgcgccgac ccgctcggcc tcgtgctcat caggcgcgga
     4141 gggtatgccg tggggctggc tcgcggtggc gtgctgcaga cgtccaaggc cgggcgacgc
     4201 catgtccagg gtcggacggc cgccggcggc tggtcgcagc agcggttcgc ccgacgccgg
     4261 gagaaccagg cggatgccct cgtcgaggcc gtcgtggggc acgtggagcg gatcctcgtc
     4321 aagggggagg gcggcgttcc ggacggtctg gtgctcggcg gcgaccgggc cctggcagcg
     4381 gctgtgctcg ccgacccgag gtgccgggcg ttgcgcgacc tgccccgacg tgagttgccc
     4441 gaccttcccg accctgacgc acgggtgctg gcggaggcgt tgcgccgcgg ccggtcggtg
     4501 tggatcgtcc cggtgcccca gccgagctga cccgaggctc agttgaccgt gaaggccttg
     4561 gtgtcccaga cctgcaggga agcgtcccta ccgttgtcgt tgtaggcacg cagttcgtag
     4621 gtgcccggcg acagggtgat gctcgtctgg gcctccgcga aaccacccat catcgtcgtc
     4681 acctgggtcg atgccacctc ggtcgccccc tggaacactt tgagcggcac gttgccctcg
     4741 aagcccgtgc cgtagacctt caccacgacc ggtgagcccg tgctcaggcc ctgggtcggg
     4801 gcgaggatcc agacgttggc gatctggttg agggcgtcgc cacgccgttg gatcccggtg
     4861 aggtcgaggt gccccgagga gggcacggaa ccgttgaccc ggaccttgac cccggtgatg
     4921 gacgcatccg ctgcggtcac ggtgtagacg agttggttga ctgctgccgc ttcgtaaccg
     4981 gaccccagcg tgcccggata tgcggagagg tcgacggtcg ccacgccggc agccacgctg
     5041 acggacttga ccagcgtcgt ggctggccac agggtcgagt agtcggggtc gctcgggggc
     5101 agcgagagca tggcctgcac cgccgtggcc accgtgcctg acggcagcgg agcgagttgg
     5161 cggaactcgc ggtagaggcg cggtccggca aggccggtga cctcgtcgac gtaatagacc
     5221 gggacggtca ccggggtggc cgtcgatgtc gaggaactgc tcgtgctcga tggggtcgtc
     5281 gttgccgtgg ccgtcggcga tgggctcgtc gaggtggctg acgtcggggc ggacgcagtg
     5341 accgtgacgg tcggcgacgc gacgggtgag gacccggcat tgccgcggat gaagatgaag
     5401 gcgaccaacc cgatgatcac cacggccaac gccgcgatga cgggggcgag cgagcgcgcc
     5461 gcttccgggc cgggggcgcc cgagtcgtcg ggcggaccac ctgagggggt cgatgggtcg
     5521 aagctcatgg gattcagcct cctggatcgc cgcggcgctc accccacggc gcgcggggtg
     5581 ctcgcggctc catctgttgg acgcctgccc gatccagcag gttgaccgac cgtgatcgaa
     5641 ccgactccgg cgcgcgcccg gccgcccgcc cgacggcgag ccccactgcc taggatgggc
     5701 ccatgagtca ggactaccgc atcgtcgagc tgagcacgca ggaatgctgg gagatcctgg
     5761 accgggaacg gttcgggcgc ctggcggttg ccgtcaacgg cgaccccgac atcttcccca
     5821 tcaacttcct cgtcgacgac cagaagttgc tcatgcggac ggctccgggc accaagctca
     5881 ccgagctcgt catcaactcc gcggtggccg tcgaggccga cgggcgcgac ggggacacgg
     5941 cctggtcggt cgtcctcaag ggcgtggccc gcatggtcga ctccttctcc gagacgtatg
     6001 ccgatgacgg caaggccttg gagacctggt tgcccagcga gaagccgatc tatgtcgaga
     6061 tcatcgcgcg ctcgatgagc ggccgccggt tcctgcgcgg ttcggcggtc tagcctcccc
     6121 ggccttcccg gccggcccca cagacgcgag tgggcggcat accccagggg tatgccgccc
     6181 actcacgtga gggggagggg gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnncttctgg gggagtgtca ctccacgcgc gaaaccccga aggtgttgca ctggttgatg
     6361 tcgccgctgg tgtagccggc catgaaccac ttctggcgag cctcggagga cccgtgggtc
     6421 cacgactccg gggagacccg accctgcatg gtctcctgga tccggtcgtc accgaccgcg
     6481 gaggctgccg agagggccga ctggatgtcc ttctgggtga tcggcttgag gaacgcggtg
     6541 ccgttggcgt ccttcgtctc ggtcgcgtgc ttgacccagg tgccggccag gcagtcggcc
     6601 atgagctcca tgcggaccga gccggactga gggccctgcg ggtcctgttg agcctttccg
     6661 aggatgccga ggatgtcctg gatgcggtgc ccgtattcgt gggcgatgac gtattccttg
     6721 gccagttggc caccatcggc gccgaactgg ctggtgagct gggcgaagaa ggacgcgtcg
     6781 atgtagatct tctcgtccag cgggcagtag aacgggccca cctggttgga ggccgtgccg
     6841 caggccgact gagtggatcc ggagtagagg acggtcggcg tcatcgggta ctgcttgccg
     6901 tattgcggga gcagctgggc ccaataggcc tggacgctgt tgacggtcgc cacgaccaga
     6961 cactggtcgt cgcggttggc gtcggcaccg gtcttgcact tggcgaagtc gttgttctgc
     7021 tggccgcccg cgctgacctg gctctggtcc agggggctgc tctgcgtgcc ggtcgtcgtc
     7081 gtgtcgggca cctgggtgcc gccaccgccg atgacgccgc cgccgaagat catcatgagg
     7141 atcagcagga tcaggccgcc gatgccgccg ccgacctgga tgccgccggg gaacgatccg
     7201 ccgccgcctc gccccagacc gccgccgccg ccgcccccac ccgactcaat ctgtgaggtg
     7261 tcgagttgga cgttctcgtt gaaggacatg cacccctcca gtcatgtggg cggatggacc
     7321 gccgcgtcac ccacacaccc taacggccag agcgtgcgct gccttcccgt gacgggcccg
     7381 cggagggtca cgactcgggc acgtccggcg agccccaaga gcggttgcgg gtcggtcccg
     7441 gtgcccgcgc ctggcagaat agggaggtcc agcttgtcgt cgccgttccc ggcgcccccg
     7501 acccagaggt atgccgtgat cgtcgccacc gccgtgcagt tgcgcgtggg cgcccgcttg
     7561 ttgctcgggg agacgtcgct gcggatctcc gagggcgacc ggatcggcct ggtcggacgc
     7621 aacggggccg gcaagaccac gctgaccaag gtcctcgccg gtgaggcgct cgcgacctcc
     7681 ggcaccgtga cccgcaccgg cgccgtcggc tacctctcac aggacccccg gaccggtgac
     7741 ctcgaggtgc tcgcccgaga tcgcatcctc gccgcccgag gcctggacgt cacgttgcgc
     7801 gagttgcgcg cggccgagac ggcgatggca tcgagcgacg gcgcggtgca ggaacgcgcc
     7861 atgcgcaagt acacgcggct ggacgcggag ttcaacgccc aaggagggta tgccgccgag
     7921 tcggaggccg cctcgatcgc ctcgtccctg gggttgccgg accgggtgtt gggccagccc
     7981 ctacggacgt tgtccggtgg ccagcgccgg cgggtcgagt tgtcgcggat cctcttctcc
     8041 ggcgcccaga ccctgctgct cgacgaaccc accaaccacc tcgatgccga ctcgatcggc
     8101 tggttgcgcg actacctcaa gtcctacaag ggcgggctgg tcatcatctc ccacgatgtg
     8161 gcgatgctcg acacggtcgt caaccgggtc ttccacctgg atgccaaccg gtccgagatc
     8221 gacatctaca acgtcgggtg gaagccctac ctccttcagc gcgagacgga cgaacgtcgg
     8281 cggcgccgcg aacggctcaa cgcggagaag aaggcgtcgg ccctgctggc ccaggccgac
     8341 aagatgcggg ccaaggccac gaaggccacc gctgcccaga acatggcgcg tcgggccgaa
     8401 cgactgctgg ctggcatcga gggggagcgc gcccacgacc gggtggccaa actgcgcttc
     8461 ccgagccccg caccgtgcgg tcggactccc ctcaccgcag aggggctctc gcgctcctac
     8521 gggtccttgg aggtcttcac cgacgttgac ctggccatcg atcggggctc gcgggtggtc
     8581 gtgctcgggc tcaacggtgc cgggaagacg acgctgctgc gcatcctcgc cggggtcgac
     8641 gctcccgaca cgggcgaggt gcttccgggg cacggcctca aactcggcta ttacgcgcag
     8701 gaacacgaga acctcgacgt cgagcggacc gtgctggaga acatgaagac cgccgcaccg
     8761 gatctcggcg agaccgaggt gcgcaaggtc ctcggctcgt tcctcttcac cggcgacgac
     8821 gtcgacaagc cggccaaggt gctctccgga ggagagaaga cgcggttgtc gctggcgatg
     8881 ctcgtcgtgt cggcggccaa cgttctgctg ctcgacgagc cgaccaacaa cctcgacccg
     8941 gcctcgcgcg aggagatcct cggcgcactg cggacctacg aaggcgcggt cgtcctcgtc
     9001 acccacgacg agggtgcggt cgacgcgctc gaacccgagc gggtactgct gttgcccgac
     9061 ggagtcgaag acctgtgggg cgccgactac aaggacctcg tctcgctcgc gtagcgggtc
     9121 tccttatccg ggcaaccccg ccagcgtggt gccttgcgca gggcggcgtc tttcggagct
     9181 cagatttgct acgctaagcg tgttaaagca acagatctga aacttaaggg cacatatgac
     9241 tgccgcgacg actgggcgca cccctgtggc ggtagcgcgc gcgctggacg ggctgggcag
     9301 ggacctgacc acatggcgca aattgcgcca cctgacggcc gagcaggtcg cggaccgcgc
     9361 cggcgtcagc cggagcaccg tcctgcgcct ggaaggcgga cagggagctt ccgtggagaa
     9421 cctgctgcgg gtggctcgcg cgctgggtgt gctggaactg ctcaccaagg ccctcgaccc
     9481 gtacgccacc gacgtgggtc gcctccgtgc cgaagagaag ctccccacca gggtgcgggc
     9541 gcgcggagta agcaggtgag cgctgtcgag gtctgggtgt ccatcgaggg cgtcgacgtg
     9601 cgcgcgggca cgctgtaccc gcaccgccgc cgcggtggcg ccaccgagag cgccagcttc
     9661 acctacgtgg acgagtacat cgcgaacccg cgcgcttact ccctggaacc gggactgcca
     9721 ctcaaggccg gggctcacca gagcaccctg gggcatccga tgttcggcgc gttcacggac
     9781 tgcgccccgg accgatgggg ccggactctg gtcaaacggt gtgaggccgc ccaggctcga
     9841 gccgcaggcc gcgaaccgcg cagcctcggt gaggtcgact acctcctcgg ggtccgcgac
     9901 gacctgcggc agggcgccct gcgcttccgc cgtggtgacg gaccgttcga ggcggtgggg
     9961 gaccgtggcg tgcctgcact gaccgacctc cctgagctgc tcaccctcgc cgcacgagcc
    10021 gaggcggact cagccgacct gccagacctt cggcgcctca tccgcgtcgg cagctctctg
    10081 ggcggggcgc ggcccaaagc gcacgttcgc ggcgccgacg gcaccctcgc catcgccaag
    10141 ttccccagcg cggcgcacga cacgtggaac gtcatggcgt gggagaaggt cgcgctggaa
    10201 cttgccgccc gggccgggat caccgttccg gccagtactc tgctgaacct cgcggaccgc
    10261 agcgttctca tcgtggaccg gttcgaccgg acccgcgatg ccgacggcgg agccaccggc
    10321 cgggtcggct acgtgagcgc gatgtcgatg ctgcggtcca ccgatggaga gcagggcagc
    10381 tacctggaga tggccgaagc catcgagatg cgctcgtcga aggctacgac ggacctgcac
    10441 cagctctggc gccgcatcgt cttctccatc ctcatctcca acaccgacga ccacctgcgc
    10501 aaccacgggt tcctccacgc gggcggcgac gcctggcggt tgtctccagc gttcgacgtc
    10561 aacccgaacc ccgaaccggg acccaaacat ctgagcacgg ccatcgacct cgccgatgac
    10621 acggcgagca tctcgcttgc gctctctgtc gcggactact tccgcctcgg tgctgaggcg
    10681 gccaacgacg tcgtcgtaca tgttcaggcg gtcgtctcgc agtgggccac ggtcgcccgc
    10741 cagtatggcc tttccgggca ggaagtctcg aagatgtcgt gggcattcga ggcgcactcc
    10801 gcgcgccccc acccgtgatg gcaaggggtg accttcacga gccttcgcct agcctgcgct
    10861 agcggccgtt cgaagctgtc ccatgtgaat aaccaaacgc ttgagttcac ataatggacc
    10921 attatgcaaa tgaggggtga gtgccccatc atggagtcat gagcggtgca cgcctcgtct
    10981 cccgacgcca tgtcgacctt cttcgggtcg ccagcgccgg atgtcgacgg tgccggctcg
    11041 cctccgacat gcccttggcc ctccggcgcg gctgactccc cgagtcgctg tctgccaggt
    11101 agggcctgca taccggactg atccgccaga cgcgccgccg cggcataccc ctgccgggcc
    11161 gacgcccacc tggcccacct gccgagccgt tgtggccacc ggcgctggca cgcaggcgtg
    11221 ccggaaccct ttctcctccc acccgacggg atcactgacg catgtccaca cccctcacga
    11281 ccgccgcccg cgccactggg gcctgggccg acggcgaccg gacgccgctc aacgacaacg
    11341 agcgcttcaa gctcgaggac gacggcctca acgtccggca gcggatcgag acgatcttct
    11401 cccgcgaggg cttcgggtcg atccccgtgg acgacctgcg cggccggatg cgttggtggg
    11461 ggctctacac gcagcgccgt cccggcatcg acggcgggca gacggccaag ctcagcgccg
    11521 acgagttgga cgactcctac ttcatgctgc ggatccgatc tgacggcggt gccctgtccg
    11581 aggtccaact gcgcaccatc gccggcatct ccaccgagtt cgcccgcgac accgccgata
    11641 tcagcgaccg acagaacatc caactgcact ggatccgcgt cgaggacgtc ccggagatct
    11701 ggcggcggat cgaggccgtg gggctcaaca gtgctcaggc gtgcggcgac gttccgcggg
    11761 tgatcctcgg ctccccggtg gcgggcatcg acgtccacga gatcatcgac ggcactcccg
    11821 ccatccaaga gatcctcgac cgctacctcc tctcgccgga gttctccaac ctgccgcgca
    11881 agttcaagac cgcggtgtcc ggctcgccga gcctcgacgt cgcccacgag gccaacgacg
    11941 tgtcgttcgt cggcgtggtt caccccgagc acggccccgg cttcgacgtc tgggtcggcg
    12001 gcggcctgtc gaccaacccg atcttcgccc agcgcctggg cgcgtgggtg ccgatcgagg
    12061 acgtgccagc ggtctgggcg ggggtcgtgg gcatcttccg tgactacggc tatcggcggc
    12121 tgcgcaaccg ggcccggttg aagttcctcg tctccgactg gggagccgaa cgcttccgtg
    12181 aggtcctgga gaccgaatac ctcggacggc gactcctcga cggaccggct cccgtggagg
    12241 accccgaacg ccgtcgtgac cacctcggcg tccaccccca ggtggatggc cgcttctggg
    12301 tcggcgtggc tccgatcggc gggcgcgtct ccggcacgac cctcactgcg gtcgccgacc
    12361 tggccgccag gcacggctcg ggccgtgtcc gcctgacggc ccaccagaag ctcctcgtcc
    12421 tcgacgtccc cgaggccgag gtggcgaccc tcgtcgccga gctcgacgcc ctcggtctgc
    12481 ccgccaaccc cagcgagtgg cgccgcggcg tcctggcctg caccgggatc gagttctgca
    12541 aacttgcgct ggtcgagacc aaggcccgcg cccgatcggc cgtcgaggag ctcgaacgtc
    12601 ggatccccga cctcgacgcg cccgtgacca ttcacgtcaa cggctgcccc aactcgtgtg
    12661 cccgtttcca ggtcgccgac atcggcctca aggggctggt catgacgggg gcctcggggg
    12721 agaccgtgga gggcttccag gtccacctcg ggggcgccct cggcggcaac gcccggctgg
    12781 cccgcaagac ccgcgccctg cgggtggagg cggacgacct cgtcgattac gtcgagcgcg
    12841 tcgtcacccg cttccgcacc gaccaggccc ccggcgagtc cttcgccgaa tgggctcacc
    12901 gcgccgacga ggaggccctc cgatgagcgg caccccctac ttctgccccg tgtgcggaga
    12961 gcaggacctg cgccccgacg agaccgacga gcagtcgtgg gtatgccgtg cctgcgcctc
    13021 ggtcttcagc gtcaccattc gcaggagcgg gcgatgagcg ccgcgacccg gtcggtgacc
    13081 gacctgcgcg aactcgccca cgagggtgct gcgctggtcg gggacggcga cggaccggca
    13141 tacgaggatc gcgtcgaggc cgctcgtgtg gccctgcgct gggcccgcct gatctttggc
    13201 gaccgcctga gcgtcgcgtc gtcgatgggc gacgaagtgc tggtgcacct cgtcgggcgg
    13261 accgtccccg ggaccgatgt gttcttcatc gacaccggct accacttcgc ggagacgctc
    13321 ggcacgcgcg acgcgtatgc cgcgaccggc gaggtgcggc tgcgcaccgt ccttccgctg
    13381 ctcaccgtgg ccgagcaggc ggagcgacat ggcgaccggc tcttcgagcg cgaccccgac
    13441 gcgtgctgcg ccatccgcaa ggttgagccg ctggaacggg cactcggagg gctacaggcc
    13501 tgggtcacgg ggatgcgccg cgaggacgcc ccgacccgca ccgacatcgc cctcgtcggc
    13561 tgggatgccc ggcgcgacct ggtgaagctc aacccgctgg ccgcctggac ggctgccgac
    13621 gtcgaccgtt acacccgaga ggaatccgtc atggagaacc ccttgcgcct ggatggctat
    13681 gcctccatcg ggtgcgagcc gtgcacccgg ccggtggccg agggggaaga ccctcgagcc
    13741 ggccgctggt ccgggcgcac caagaccgaa tgcgggttgc accagtgacc gccgtggtgg
    13801 ggacgtcccc tgcagttgag acacccgccg aggcgtcggc gcgcccgagt gtcgggcgcg
    13861 cggcataccg gctggaccac ctggaccacc tcgaggccga ggcgatccac gtcgtgcggg
    13921 aggtggccgg gcagttcgag cggccggtga tcctcttctc cggcggcaag gactcggtcg
    13981 tcatgctgca tctcgccgtg aaggccttcg cgccgtcgcc gttgccgttc gcgctgctgc
    14041 atgtcgacac ggggcacaac ttccctgagg tcctcgacta tcgcgacgcg accgtcgcgc
    14101 ggctgggcgt gcgcctggtc gtggcgtccg tgcaggacta catcgacgac ggacgactgc
    14161 gcgagcgccc cgacgggacg cgcaaccccc tgcagaccgt gccgctgctg gacgccatct
    14221 ccgggaaccg cttcgacgcg gtcctcggcg gcgggcggcg cgacgaggag aaggcgcgcg
    14281 ccaaggagcg ggtcttctcg atccgcgacg ccttcggcgc gtgggacccg cgtcggcagc
    14341 ggccggagtt gtgggacctc tacaacggtc gccacgcgcc gggggagcac gtgcgcgtgt
    14401 tcccgctgtc gaactggacc gagctggacg tgtggcggta tgtcgagcgc gagcgcatcg
    14461 agctgcctga cctctactac gcccacgagc gcgaggtctt cctccgtgac gggatgtggt
    14521 tgaccgccgg ggagtggggt ggcccgcggg cgggcgaaac gcccgaggtg cgcaccgtgc
    14581 gttaccggac cgtcggggac atgagttgca ccggagccgt cgattccgac gcgctcaccg
    14641 tggccgacgt tgtccttgag gtggccggct cacggctcac cgagcgcggt gcgacccggg
    14701 ccgacgaccg gttgtcagag gccgccatgg aggaccgcaa gaaggagggg tacttctgat
    14761 gactgccacc atcgaggccg tgccgcacga gttgctgcgc ttcgccacgg cggggagcgt
    14821 cgacgacggc aagtccaccc ttgtcggtcg cctcctgcac gacaccaagt cggtcctcgc
    14881 cgaccaactc gccgctgtcg agcgggtgag ccgcgaccgg ggcctggagg ccaccgacct
    14941 cgctctgctc accgacggcc tgcgcgccga gcgggagcaa ggcatcacca tcgacgtggc
    15001 ctaccgctac ttctcgaccg ccacccgctc ctacatcctc gccgacactc ctgggcacgt
    15061 ggagtacacc cggaacatgg tcaccggggc gtcgaccgca gagcttgcgc tcatcctcac
    15121 cgatgctcgg cacggcatcc tcgaacagac ccgccggcac ctcgcggtca cggggctgct
    15181 cggtgtgcgg cacgtcgtgc tggccgtcaa caagatggac ctcgtcggtt gggacccgtc
    15241 ggtctaccac cggattgccg acgaattcac caccttggca agggagttcg gcatctcgga
    15301 cgtcaccgcc atcccggtct cggcgctgct gggcgacaac gtggtcgacc gctcggagca
    15361 cacgccctgg tatgccggtc cgaccctcct gcagcacctc gaagaggtcc cggtcggttc
    15421 agacccgagc gagcagccct tccgcctgcc ggtccagttg gtgatccggc cgcggacccc
    15481 ggcgcatccc gactaccggg ggtatgccgg tcggctcact tcaggggtcg tccgcgtggg
    15541 cgacgaggtc gtcatccacc cctccgggcg tcgctcgcgg gtggtcggcg tcgacctgcc
    15601 tgccgttgac ggtcaggtcg tgtccgtggc cgaggcggcc gcgccccgct cggtgaccct
    15661 tcgcctggcc gacgacatcg acgttgctcg cggcgacctc atcgcctcgc cgcaagacga
    15721 acccacggta tgccgcgccc tctccgggcc cgtcgcggtc ctctccgacc ggccgctgcg
    15781 ggtgcgtgac cgggttctgg ccagggtcgg cacccggacc gtccgcggca tcgtcgagtc
    15841 gatcgacgac gagttggaca tcacgacgat gacccacctc ccgacgggtg attcgttggg
    15901 gctcaatgcc atcggccggg tgacggtgcg actggccgag cccgtggcgc tggacgacta
    15961 ccggcgactg cggcacaccg gggcgttcct gctcatcgac gaggtcgacg gcacgacgct
    16021 ggccgccggg atggcgcagg agtcctgtct gggctgaccg cctccgccca tcgatcgcct
    16081 caaaccccaa ggaactcccc atgtcttcca cccacatctc ccgcgtccgc cgtccccgcg
    16141 tccgcgttgc gttgagcctg ctcgcgttgc tggccacgag cgtcgtgtcg gcttgctcct
    16201 cctcgagcca ggccgcctcg accggcggtg cctccacgtc cgcggccagc accctgcgcc
    16261 tgggctattt cgccaacctc acccacgcga cagccgtcct gggcgtcaag gacggccact
    16321 tcgccaaggc cctgggaacg accacgctgc agacccaggt cttcaacgcc gggccggccg
    16381 cagtcgaggc gctgctcgcc ggagccatcg acgcggcata cgtcgggccg agccccgcga
    16441 tcaacgccta cacaaagtcc ggcggtgctg cgatccgcat tgtcgccggc ggcacgagcg
    16501 gaggcgcgtc gttcgtcgtc aagccgggcc tcaccgaaac caccctggcg ggtaagacgt
    16561 tcgcgtcgcc ccagttgggt ggcacccaag acgtcgccct gcggtcctgg ctcgccgcac
    16621 atggctacaa gacatcggtg agcggcggcg gtgacgtcac ggtcgctccg caggacaact
    16681 cccagaccct cagcctgttc aaggcagggt cgatcgacgg cgcctggctc cccgagccgt
    16741 gggccacccg cctgcgcctg gaagccggtg ccacaaccct cgtcgacgaa acgacgctgt
    16801 ggccgcaagg tcgcttcgtc accaccaacc tcatcgtctc aacgaccttc ctgcaggctc
    16861 accccgatca ggtcaaggcg ctgctgcagg gcgccgtagc cgcggatgcc gccatcgcag
    16921 ccgatccgtc cggatctcgc gacaaggtgg gcgcagcgat cacggcgctc accagcgcca
    16981 aaatctcgcc cgccctgctt caagaagcct ggacgcacct gaccatcacc cctgacccga
    17041 tcgcatcgtc gctgcagacc agcgctcagg ccgccgtcac cgtcggcatc agtaagtccg
    17101 tccccgatct caaggggatc tacgacctca cgctgctcaa ccaggtcctc accgccgcgg
    17161 gtcgcccgac ggtgagtgcc gggggactag ggaaggagtg acgatgagcc tcaccaccac
    17221 catcgacccg accgcggagc cggagctcga ccccacctcg ggggcggcac cgggctcggt
    17281 cgccacctcg cccgcgagcc ccgcggtcac gctgcgcggg gtcaccaaga ccttcgaccc
    17341 ggcccaggga ccagtcatcg aagacctgtc cctcgaggtg gccaagggcg agctcgtcgc
    17401 ggtcctcggc gcgtccgggt gcggtaagtc caccctgttg gggctcgtcg ccgggctgga
    17461 tcggccgacc agcggccaga tcgtcactcc gggcggccga cccgcgctga tgttccagga
    17521 cgcctccctg ttcccgtggc tcacggcccg gggcaacgtc gagctcgcgc tgcgcctgcg
    17581 ccgcggcgaa gcccggcgca accgcaacga ccgccgggct gaggccgatc ggtggctgga
    17641 cctggtccgc ctcgggcagc acgccgacaa gcgcccgcac gagatgtccg gcgggatgcg
    17701 ccaacgggtg gctctcgccc gggccctggc ccagggcgcc gatctgctgc tcatggacga
    17761 gccgttcgcc gctctcgacg ccatcacccg cgacgtcctc cacgaggagt tgggccggat
    17821 ccgtgccgag accggcctca ccgtcctctt cgtcacgcac aacgtccgtg aggccgtgcg
    17881 cctcggcgat cgagtggtgc tcctggccag ccgcccgggg cggatcgtcc gtgaatggac
    17941 ggtccccatc ccgcatcccc gacgaatcga gtccccggcg gtcggagcct tggccgtcga
    18001 gatcaccgag caactgcgtc aggagatccg ccgacatgcc cgctgacctt gccgaccgca
    18061 ccgccgacgc gacgccggcc cctatcgacg agacagaggc gctagaggcc ggcctcgacg
    18121 tcctggatgc gcccacccgc accaccggac cacgcgcccg ccgcgtcctg gcgagggtgg
    18181 cacctccagt catcgccgcc gccgtcctgc tgttggcctg gcaggtgctc gtctcgtcgg
    18241 gactcaagcc ctcctacgcg ctccctagcc cgtcggccgt ctggacgtcg ctggtggagc
    18301 aactccagca ggggtcggcc cagcgctcca tcgtcgggtc gttgcgccgc gccgcggtgg
    18361 gcttcacgat ctcgatcatc ctgggcacgc tgctggggat cgtgctctcg aggttccgcc
    18421 tcatccgacg ggggatcggc ccgctcgtca ccgggatgca gtcgctgccg agcgtcgcct
    18481 gggtccccgc ggcgatcctc tggttcgggc tcaccgacgc cgccgtctat gccgtggtcc
    18541 tcctcggttc ggtgccctcc atcgccaacg gtctgttggc gggcatcgac cacgctccgc
    18601 cgagctaccg tcgggtcgcc cgggtgctgg gggcaggaac gctcgccgaa ctnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgccgagc taccgtcggg tcgcccgggt
    18781 gctgggggca ggaacgctcg ccgaactgcg gtgggtgacc ctcccggcgg cgctaccggc
    18841 atacctcgcc ggtctgcggc agggctgggc cttcgcgtgg cgttcgctca tggccgcaga
    18901 gctcatcgcc tactcgccgc aactcgggcc cggcctgggc caactgctcg acaccggccg
    18961 tgaactctcc gacatgcccc tcgtgctcgc gtcgattctg ctcatcctgt tcgtcggcat
    19021 cggggttgag gcactgatct tcggccccgt cgagcggcga ctggttcgca cgcgcgggct
    19081 ggagggcggc tgaatgggat accccctcac cctccagtta cgtgaccgcc gcgtcctcgt
    19141 cgtcggtggt ggccgggttg cggccacccg ggtatgccgt ctgctcgccg acggcgccca
    19201 cgtcaccgtg gtggcacccg aggtgggtga ggtcgtggcc gcactggtcg ccgagggccg
    19261 ggtgacctgg ctcgctcggg gctatcagtc gaccgatttg atgcagccgc agcgcgcctg
    19321 gttggtgcac accgccaccg gggaccacac ggtcgactcg gcggtcgcgg acgactgcga
    19381 aggccttggg atctggtgcg tgcgggcgga cgacggcgcc cgttcggcgg cctggagccc
    19441 ggcggtagcg aacgggtcgg ccgggacgcc tgctgaaggt ctgcagatcg ccgtcaccgg
    19501 cgaccgggac ccccgacgag cccgagcggt gcgcgacgcg atcctcgccg gcctcgagtc
    19561 tggtgtcctt ccggtgcggc gggtgcgccg ggtacccggg acgatgaagc cggggcgggt
    19621 ctacctcgtg ggtggcggac ccggcgaccc tgggctgttg accgtgcgtg gtcgagcact
    19681 gttggccgcc gcggacgtcg ttgtcgccga tcggctcggt cccaccgacg tcctcgccga
    19741 cctcgaccct gacgtcctcg tgctggatgt cggcaagcgc cccggcgcac atccggtgcc
    19801 ccaggaggag atcaacgccc tcctcgtcga gcacgcctcc gccggtcgga ctgtcgtgcg
    19861 actcaagggt ggtgacccgt tcgtcctcgg acgcggaggt gaggaggcac tgcactgcca
    19921 ggcacacggc atacccgtcg aggttgtccc cggcgtgacc tcggccgtgt ccgtccccgc
    19981 agccgcgggc atccccgtga cgcatcgggg gatcacgacg tcgttcgtcg tctcgtcggc
    20041 acacgacggc ggtgcggtcg ccgcgcaggc gcccctagac gccaccctcg tcctgctgat
    20101 gggtgtgtcc gggatcgggg agacggcggc tcggctcatg gacgccggac gcgatccctc
    20161 gacacccgtg gcgttcatcg agcgtgggtg gacaccgacc caacgcacga cggtgacgac
    20221 cctggagaag gccgcctggg cggtcgagca cgagcaggtg aagtctccgg ccgtggtcgt
    20281 cgtgggcgac gtcgtgcggt tgcgcgagac gctcggcgac cttcgatgag cgagcgcgag
    20341 ccgatcattc tgctcgcgca cggctcgccc gacccgcggc atgccacggg gatcgccgcc
    20401 ctggcgacac gggtggcggc tgcgggggag gcggccgtcc acgtcgccta cctcgaccac
    20461 cacggaccga cccccgctga cgccgcccgg gctgccggga gaggggtggt cgtccctgcg
    20521 ctgctctcct tgggctacca cgcgcgggtc gacgtgcctg cggcgatcga ggcgatgcag
    20581 gcggctgcgc cgggtccctt ccgggcggcc agcgggctgg gtctcggccg cgcggtgctc
    20641 gacggcgtgg gggagttgct ggtccgagcg gaggtcgccg acgtccggga cctgcacgtc
    20701 gtcctcgccc tcgccgggac taccgcgccc gaagcgatag aggagctcag cgccgccctg
    20761 acggtcgcga gtgcggcggt ggacggcccg acgtgggctg ccctcgcgtc ggtcaccgag
    20821 ccgacccacc tcgtggggta tgccggtccc gcggcggacg gcgtcccaac ggtcgtcgtg
    20881 ccggttgctc tctgcgaagg ggtgctgcgg gaccgagtca tcacggcggc cctcggcctg
    20941 ggcctcgaca cggtggcagg ggtgctcgcc gagaccgact ccctggcgcg actcgttgtc
    21001 gcgcgtgcca cgcaggcgct gctgggcacg agctgacgat gcgccggggg tggtcaggcc
    21061 ctcagaaccg gccagcccgg gcagtagaga cagaacggat ggcccgcggg gtccagcaac
    21121 acccgtacgt catgctgcgg ctggtatgcc gcctgggtcg ccccgagcgc gacggctgcg
    21181 ccgaccgcct catcgaggtt gtctacctcg atatcgaggt gaagttgcat ctgctggcga
    21241 ccaggctcat tgggccaggt cggtggcacg taaccctgcg aggtctggac cgcgaggtag
    21301 cccaccccct cgccggggtc gagggtgacg aaactgggat cctcggcatt gagcacccat
    21361 ccgaagacgt ctgcatagaa ctggccgaga gccttcgcgt ccggggcgtc aaggaccacg
    21421 ccccaccagt ctcgccgggt cttgcggtcc tcaccggggc tgcccggagt tcgtccacgc
    21481 atgcctcatc gtggcacccg cgggcgtctg cgcggaatga gccagcccgg tcagccgggc
    21541 cggctccgcg gaatgggtcg ggccggtcag ccggggatgg tgggcaggtg ggtcagcgcc
    21601 tcggcgaccg cgtcacggat ctcggcctcc tcgtaggagt ggtcgctcat ctcgcccgcg
    21661 aggtaggcac cgtaggccgg caggtcgagg tgaccatgac cgcagagcgc cgtgaggatg
    21721 accttctcct cgccggtctc cttgcaccgc aacgcctctg cgaccgtctc ggccagcgcg
    21781 tgggtgggct caggtgccgg gacgatgccc tcggtgcgag cgaagagcac accggccgcg
    21841 aagcactcga gttgcttctt ggacaccgcc tcgatgagcc ccgactcgta gatgtgcgag
    21901 atgagggggc tcatcccgtg gtagcgcagg ccaccggcgt ggatcgggtc ggggatgaag
    21961 tcgtggccca gggtgtgcat cttcatcagc ggggtcatcc ccgcggtgtc gccgaagtcg
    22021 taggcatagg tgccctgggt gagcgacgga caggatgcgg gctccaccgc ccggatgacc
    22081 ggcgacatct tgccggcgag cttctcgcgc aggaacggga aggacaaccc ggcgaagttg
    22141 gacccgccgc cggtgcagcc gacgatgagg tcgggcgtct cctcgatctt ggccagctgt
    22201 cgcaaggcct cctcgccgat gatcgtctgg tgcaacagca cgtggttgag caccgatccg
    22261 agtgcgtagt tggtcgactc ggactgggca gcgatctcga cggcctccga gatcgcgatg
    22321 cccagcgatc ccggcgagtc ggggtgcgcg gcgaggatgg cccgcccggc tgcggtcaac
    22381 tccgacggtg acgggtggac cgtgccgccg aacgtctcca tcatgagccg gcgatacggc
    22441 ttggagtcaa agctggcccg gacctgccag atctcgcact ccagcccgaa ctgcgcacac
    22501 gcgaaggcca gcgacgtgcc ccactggccc gcgccggtct cggtcgtcag cttcttgatg
    22561 ccggccttgg cgttgtaata cgcctgcggc acagaggtgt tgggcttgtg cgagcccgcg
    22621 ggggagacgc cttcgtactt gtagtaaatc cgcgcggggg tgcccagggc cgcttccagg
    22681 cggtgtgccc gatagagcgg cgacggccgc cacagtcggt agacatcgag gacctccggc
    22741 gggatgtcga cgtactgagc cgtcgtcatc tcctgctgga tgaggtccat ggggaagagc
    22801 gcgagcaggt cctccggccc gaccggttgt cccgtcccgg ggtgcaggta gggcggcggc
    22861 ggtgtgggga gatcgtgaag gatgttgtac cagcgggtcg gcatctcccg ctcgtcgagg
    22921 atgaccttgt gccgcttgac agtgctcacg ggactctcct tcgcgtcggg gcgctcgggg
    22981 cacatccttg cgcgtccgcc gcgcgcactc caagccgggg tcgcgtgatg ggcacggcgg
    23041 catacttcgg ggatggtcac ctccctcccg aggtatgccg cgaggtatgc cgcgcgccgc
    23101 acctcgggcg tgcaacctcg ggggaccagg gcgcgcaggg ccttggtgac cctcacggtg
    23161 ctcacggtga ctgccgtgac tgggtgctca gccgggcagg gcgacaggtg gagcgcgctc
    23221 gggccactgt cggcccaggt cggtgcagtg gtgaccctga cggcggtggg ggacctgctc
    23281 gtggcaggca ggtcgaccgg ggcgggtgag cccgccttgg aggtcctggc cgatggcacc
    23341 tggaccagcc ggccggtcca ggccgtcacc ccctacggcc gcgtcgctca atggctctcg
    23401 atcgccgctg gacctgatgg cagcctcgtt gcggtcggcg gggctcgggg cggggcccac
    23461 gcaaacgtcc gctggacggt ctggcggggc agcgtcgccg ccggcctgac cgagcaggag
    23521 cagaccttca gcaccttcgg tggttggggc gcgggcgatc aggtcggccc ggtcctcact
    23581 gcggccggac cgctgctcgt cggatcgtgg gagggcgccc aggccgggtt ggacctcgcc
    23641 acttgggtgc ccgacggcga ggcctgggtg cgagagccat cggcgggaac ggctttggag
    23701 agcaccccaa ctcagctaat gggggcgcgc tccgtcgccg cgtggggatc cggcgccctg
    23761 atcaccggct cggtcgtgcg gctcggcgac ggggcggttc gcaaggtccc tgcgatctgg
    23821 cgctccacca ccggcaacgc cggctggacc cgcgtcgacc tgcccgactc ggggcaggca
    23881 ggggaggccg tcagcgcagc ctgcaccgag gcggagtgca ccgtcgccgg ttgggtcgac
    23941 ggccggctgg ccgtctggcg ggtgggtgcc gatgggggcg ctcggcgggt ggacggcata
    24001 ccctccgtcg ctgtccgcga cagcgacctc atcccggcac cggcgttcgt cgaggggcac
    24061 gcggttctgc tcgtcaccga ccacgactca tcgcgcaacg cgacagtcgt cgtcgaaggg
    24121 cgcaccggat ggtcccgcgg gcccggcccg tggggcacag tccaggcgtt caccgcgatc
    24181 ggatcgaccc tgtatgccgt gtgccagcac ggggacacgc ccctgcaggt ctgggcccac
    24241 aagctctgac ccgcggcttc actcccgcgg gctcagaccc gcggggctat gtctgcaggg
    24301 cagggcatag cctcctggtg tgacctcgtt gacgcagccg caccccgcca tccgcgtcga
    24361 gcaggatggg ccggtcgtga ccgtgaccct gtgcaacccc gagcacctca acgcccaggt
    24421 gccgtcgatg tggctggccc tggccgacat cgcccacggc ctcgcgggcg aggtgcgggt
    24481 cgtcgtcctg gccgccgaag gacgcagctt ctcggccggc ctcaaccgcg ggatgctccg
    24541 cccgggaggt atgccgggtg agcccgacct gctcgctgct gcagcgagca gccccgaggc
    24601 gatgaccgag ctcatcgcgc cgttccaaga gggattcgcg gcctggcgcc gggttgcccc
    24661 ggtcgtcatc gcagccgtcc aggggcacgc gatcggcgcc ggcttccaac tggcgctggc
    24721 cgccgacctg cgggttgtcg ccgacgacgt gtcgttcgcg atgcgcgagg tgagcctggg
    24781 catgatcccg gacctggcgg ggacgcggcc gctcgtggag gcggtcggct actcccgggc
    24841 gctggagctg tgcgcgaccg ggcggttcgt cggcgccgag gaagcagtgc ggatgggcct
    24901 ggcgacgctc gccgtgccgc cgaccgagct ctccgcgacg acctccgatc tcgtccaggc
    24961 cttgctgggc accccgccgg ccgcgctccg ggccctcaaa ccgctgctca accacgccct
    25021 cacggcaacg atcgacgagc aggtcgtccg cgagcgggta gcccagggtc acctcctgca
    25081 cggcatggcc cgagctgccg gcctgtcctg acccctgtcg cgccgaaacg gcgcggcggg
    25141 acggcatacc ctcggaatga cccagtcacc agtggtgttg cgccggtgac gacgacacag
    25201 aagcgccgca cgcgcccgcg aggagcgcac gtgaggcgcg gagggagggg ccgatgagct
    25261 tcggtcaggg cggccccatg ggaatgatgc gcgggatggc caaggaccaa tcggtccttc
    25321 gcgcccggtt ggagcaagga accacgcgcc gggtgctggg ctatgcgcgg ccgttcgtgc
    25381 ggtcgatcgt cgccttcctc accatcgtca tcctcgacgc cggcatcgtc gtcgctgtgc
    25441 cgctgctgct gcgcgacctg gtcgacctgg gcgtcatccc gaaggccccg gacgtcgtcg
    25501 tgcgacttgc tctgctggtc gcagccctgg ccgtggcgga cgcgatcctc agcatcgccc
    25561 agcgatggtt ctcgtcgcgg atcggtgagg gcctcatcct gcggctgcgc accgaggtgt
    25621 tcgcccacgt gctgcgccag ccgatcgcct tcttcacccg tgctcagact gggtcgctgg
    25681 tcagccgtct caactccgac gtcatcggcg ctcaacaggc cttcacctcg gtgctgtcct
    25741 cggtcgtgtc gaacgcggtg tcgctcgtgc tcatcgtcgc ggccatggcc accctgtcct
    25801 ggcaactcac gctcgcggcg ctcgccctgg tgcccttctt cctcatcccg gctcggttca
    25861 tgggtcggcg cctgtccgag ctcacccgca cggcgatgac cgccaacgcc gacctcggca
    25921 cccggatgac cgaacgcttc aacgtggccg gcgcgctcct cgtcaaactc ttcgggacgc
    25981 ccgagcggga ggagcgcgag tatgccgtgc gcgccaagct cgtgcgcgac accggcgtca
    26041 agatcgccat gaaccgcacc gtcttcatgg tggccatgtc cctcgtcgcc gccctcgcca
    26101 ccgcgatggt ttacggggtc ggcggactga tggccgtcag cggcgccctc accgtcggga
    26161 ccctgctcgc cctcgctgcc ctcctggccc gcctctacgg cccgctgacg gcgctgtcca
    26221 acgtgcgggt cgacgtgatg acggcccttg tgtccttcga ccgggtcttc gaggtgctcg
    26281 acctcaagcc gctcgtggcc gaggccgccc aaccggtaac gctgccggcc gggcccgtgg
    26341 ccgtcgagtt cgacggggtc gggttcgcct acccctcagc cgacgaggtg tccctgcggt
    26401 ccctcgaatc caccgccagc ggcgaccgcg ccggaggcgg gccggtgctc gacgaggtga
    26461 gcttcacggc atacccaggg cagttggtcg cgcttgtcgg gccgagcggc ggcgggaagt
    26521 cgacgatcac cgcactggtc gcgcgcctct acgacccgtc gcacggcacc gtccggatcg
    26581 gcggggtgga cctgcgagag gccgcgttcg gatcgctgca cgagaccgtg gggatggtca
    26641 cccaggaagc gcacctcttc cacgacacga tccgcgagaa cctccggtat gccgcgcccg
    26701 acgccaccga ggagcagatg cgggcggcgc tggaggccgc ccaggtgctg c
//
LOCUS       DAORPW010000016        24865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_321_length_24865_cov_736.953043, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000016 DAORPW010000000
VERSION     DAORPW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 24865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24865
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_321_length_24865_cov_736.953043"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /gene="era"
                     /locus_tag="PLB86_02720"
     CDS             complement(<1..854)
                     /gene="era"
                     /locus_tag="PLB86_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRC64076.1"
                     /translation="MTDQGEDAAYRAGFACLVGRPNAGKSTLTNALVGQKVAITSSKP
                     QTTRHTIRGISTTADSQLILIDTPGLHRPRTLLGERLNDLVREALLDVDVVGFCLPAD
                     QRVGPGDQFIAAELAELRRVKRKPVVALATKSDLVSRERLLTHLVAIDALGEWDAIVP
                     CSATRGDQVDEVRTVLVGLLPLSPPLYPSEARTDEPDDVMIAELVREAALEGVRDELP
                     HSLAVVVEEIVPREVPEGHAPMLDVRVSVFVERDSQKAIVIGRGGARLRDVGSTARAA
                     IEELLGSKV"
     gene            complement(847..2187)
                     /locus_tag="PLB86_02725"
     CDS             complement(847..2187)
                     /locus_tag="PLB86_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRC64077.1"
                     /translation="MSGVVLAQVVVVTVLATVLAFCLSAAEAALQRMTRVRAEELADD
                     GRVGAAALVAVVEQPAPYLAVLAFLRVVAEATTAVLITVAVIALTDSLGTALAISILV
                     MALVSFVLVGVSPRTLGRQNYERAALAAAPVTRALRRVLGPVSHGLIALGNAVTPGKG
                     YRDGPFQTESELRDLLDQASDSSVIEDDEREMIRSVFELGDTVAREVMVPRPDMVTVQ
                     GDDPLRKATAVFLRSGFSRLPVIGDDSDDVLGLIYLKDVVARITADPEAGAVACSSLM
                     RPMSFIPESKPIDDLLREMQRDQVHFAVVVDEYGGTAGLVTIEDILEEIVGEIADEYD
                     RDEPGVEPIGDGGYRLPAAMHIDDVAELFDLDIDEDEVDSIGGLLTKALGRVPFAGAH
                     AGIAGLSITAERMAHRHRIATVLVRPLVPLGETESEPLVRRLAGDPDRVGGDDD"
     gene            complement(2204..2665)
                     /gene="ybeY"
                     /locus_tag="PLB86_02730"
     CDS             complement(2204..2665)
                     /gene="ybeY"
                     /locus_tag="PLB86_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRC64078.1"
                     /translation="MSVDVLNECGYAVDELELVACARHVMDRMKVHPDADLAISLVDE
                     AAMTVLHEKWMDLPGPTDVMSFPMDELRPGRDGVDLQEGVLGDIVLCPAVAEEQARQA
                     GHAPVEELLLLTTHGLLHLLGFDHADPDEKHEMFELQRQLLLTYLAGRGRR"
     gene            complement(2662..3717)
                     /locus_tag="PLB86_02735"
     CDS             complement(2662..3717)
                     /locus_tag="PLB86_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRC64079.1"
                     /translation="MPSASVAAALPTHAITIPPTVSMVTLLGPRDELLDTIERAFPTV
                     DVHVRGNELSLRGPGVDVAVVERLIDELLVVIDAGQPLTRDAVERAIAMLRARTAERP
                     ADVLTLNIISNRGRTIRPKTLGQKRYVDAIDMHTIVFGIGPAGTGKTYLAMAKAVAAL
                     QSKRVNRIILTRPAVEAGERLGFLPGTLSDKIDPYLRPLYDALHDMVDPETIPRLMAS
                     GTIEVAPLAFMRGRSLNDAFVILDEAQNTSAEQMKMFLTRLGFGSKMVVTGDITQIDL
                     PGGTRSGLAHVRDILADVDDIYFTELTSADVVRHKLVSAIVDAYGASEAKEQAAREAE
                     DHGRHTPRHLAGGANRP"
     gene            complement(3809..4153)
                     /locus_tag="PLB86_02740"
     CDS             complement(3809..4153)
                     /locus_tag="PLB86_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRC64080.1"
                     /translation="MSAPTCLFCKIVAERIPATVVARSEHSLAFRDIDPQAPTHVLVI
                     PRRHVQNVGELADAPRELADLFELAARVADQEGLGRGYRMVTNTGSEGGQIVFHAHVH
                     VLGGRPMQWPPG"
     gene            4224..4802
                     /locus_tag="PLB86_02745"
     CDS             4224..4802
                     /locus_tag="PLB86_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HRC64081.1"
                     /translation="MQLERPGARGSARTPGAAVGVTSADEAIAILYAAHWHPLVRLAW
                     FLVHDQGTAEDVVQDAFIATHRNYDRIRDTGAAVAYLRRAVVNGARSVQRHDVVVRRE
                     AIAEAGRADAADRATAASAEAQALRHLGDGAMLAALRRLPDRQREVLVLRYFADWSEA
                     AIADALGIAPGSVKAHAHRGLAALRARLEATE"
     gene            4799..5899
                     /locus_tag="PLB86_02750"
     CDS             4799..5899
                     /locus_tag="PLB86_02750"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HRC64082.1"
                     /translation="MTAHPPGDPHVEARLRDALTTAAERVSPTDRLGAITEAVSTAPP
                     RAGTPRWLLPLAASFLVLAVGLGALTLQQRVGSNAVAASGTHTAVVIAADDPLPAPSR
                     ALPASPGASTPGSTSATRFEWSAPVYYAAPGTPVVRWALYRDFVRATMGDDGLDARVT
                     TAVNLALAQPDVWGQAMPGQDGYLRAWTPGTRVTASVAADRIALTLSAPGVGGLDADA
                     QRMAVQALVWTATAAAQRNVPVAIDVAGAGPVVGGIPAGVFRRPADTTRELAPVWITS
                     PGRFASVWDSRVVIEGQACTFEGTVQWELRLDGAMVRQGFTTATAGCPVRGTWRVDLG
                     VLAPGRYTIRAFESDAATGGPFAEQSVAFTVR"
     gene            complement(5908..6651)
                     /locus_tag="PLB86_02755"
     CDS             complement(5908..6651)
                     /locus_tag="PLB86_02755"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRC64083.1"
                     /translation="MTAPLFLVAPGSLAGAMAGTTVSIGGSEGHHAVSVHRLRIGEEV
                     LVGDGAGVRVSAEVVGVERHTLLTRVIEVQVRPPADPRFVLVQALAKGDRDEQAIEAA
                     TELGVDAVVPWQASRSVVQWRADKVARGQARWESIVVAAAKQSRRFQVPQVRPLARTA
                     DVVRLLGDAALSIVLHEEAQAPLASLDLPPTGEIVLVVGPEGGIAQTELDAFAAVGVV
                     AVRLGPEVLRTSTAGPAALAVLNGMSRWR"
     gene            complement(6695..7819)
                     /gene="dnaJ"
                     /locus_tag="PLB86_02760"
     CDS             complement(6695..7819)
                     /gene="dnaJ"
                     /locus_tag="PLB86_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRC64084.1"
                     /translation="MSDYYQDLGVERSATAEEIKRAYRRLARQLHPDVNSGAEAEEQF
                     KTVSRAYEVLSDPDKRRNYDLGVDPFAAGGGAGFSGQGFSFSDVMDAFFGGAATQRGP
                     RSRAHRGQDALVRIQIDLHDAVFGSEEELTIETAVRCSSCQGDGCQPGTGVSTCVLCH
                     GRGEVQTVQRSFLGQVMTTRPCQACQGYGSTINHPCFECSGEGRVRTRRTLTLKVPAG
                     VDSGTRIQLAGEGEVGTGGGSAGDLYVEVAVANHATYTRRGDDLHCRVELPMTAAALG
                     TTIDLQTFDGTRPFDVRPGTQAGDTMTLRGLGVTHLRGTGRGDLIVHADVQTPTRLTE
                     GQQDLLRQLAQLRGEERPIGRMAPADKGLLGKLRDAFKNA"
     gene            complement(7855..8889)
                     /gene="hrcA"
                     /locus_tag="PLB86_02765"
     CDS             complement(7855..8889)
                     /gene="hrcA"
                     /locus_tag="PLB86_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRC64085.1"
                     /translation="MSDERRLSVLRAIVQDYVRTSEPVGSKSLVERHHFGVSAATVRN
                     DMAILEEEGLIHAPHTSAGRVPTDAGYRVFVDRLSAVKPMSGAERSAISHFLQGAVDL
                     DDVVDRTVRLLASLTHQVAVVQYPSLTRSAVRHIELVPMARDRLMVILIANTGRIEQR
                     VVDAHCRLDDETGAALVSRIRSEVLGEAAGRSFPDAAARLQSLPERFEPADRDLVRIV
                     LRTLDDALAEEREERVVLAGTSNLARFGADFPLTIAPVLEALEEHVVLLKLFGQLRQG
                     QGGAVAVTIGHEHEHQGLAGASVVSAAYGTGAELVAGLGILGPTRMDYPNAMATVRAV
                     AHYVSELLEP"
     gene            9033..9911
                     /locus_tag="PLB86_02770"
     CDS             9033..9911
                     /locus_tag="PLB86_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 domain-containing protein"
                     /protein_id="HRC64086.1"
                     /translation="MDRYGRDVLADAARMPRRPVAAPVAAEPGLVVEDVETGWCGAVV
                     RVEKAGGMHVVHLEDRRGRTKGFPLGPGFLLEGRPVVLTPPVAADRAKLEAVRKAAAR
                     TASGSVAVAGQPARVAVGSRIWVEGRHDAELVEKVWGADLRVEGVVVEQLDGIDDLVA
                     SVRSFAPGPGRRLGILVDHLVPGTKESAIAAAVRALPAATQGHALLLGHPYVDVWQSV
                     KPARLGWTQWPVIPRGRSWKEGICQALGWPCDTPADLAVAWKRILGTVGSYADLEPTL
                     LASVEELIDFVTAPGA"
     gene            9939..10478
                     /locus_tag="PLB86_02775"
     CDS             9939..10478
                     /locus_tag="PLB86_02775"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HRC64087.1"
                     /translation="MSDQPANDPTRPVDVPPPPPPAWSGSAPEAPAAPVPAPPAYQAP
                     PAAYQTPAAPPPPAYPPQGYAAPVAGVVPLSPAESRQWGMLSHVGGIILSVVGPLIVM
                     LVFGPRDAFTKDQSTEALNFQITLLIGYIVATIITFIIIIPLGLVVWIAGLVFGIMGA
                     MAANKGETYRYPFNLRLIK"
     assembly_gap    10583..10682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10683..11638)
                     /gene="hemW"
                     /locus_tag="PLB86_02780"
     CDS             complement(<10683..11638)
                     /gene="hemW"
                     /locus_tag="PLB86_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRC64088.1"
                     /translation="MPSALPDGDPAPATGRLPAGALDAIASTPFGVYVHVPFCRVRCG
                     YCDFNTYAIPELGADGAAEVSGYADQVLRELDLAVDVLTDGGSGPGPRQVETVFVGGG
                     TPTLLPAADLAKVLHGIRDRWGLAPDAEVTTEANPDTVTPEGMAALAAAGFTRVSLGM
                     QSAVPHVLATLDRTHDPAAVARAVAAVRAAGMAVSLDLIYGTPGESVADWRRSLDAAL
                     ELAPDHLSAYALVVEEGTRLAGQVRRGEVAMPDDDDEASKYEVADAVLAAAGMQWYEV
                     SNWARTPDQRCRHNIGYWAGGNWWGAGPGAHSHVGGVRWWNV"
     assembly_gap    12108..12207
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12258..12704
                     /locus_tag="PLB86_02785"
     CDS             12258..12704
                     /locus_tag="PLB86_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRC64089.1"
                     /translation="MVEMTRVDAVREIAAGYAARRGPLLPVLHAVQDRFGAIERGDLV
                     AVADVLNLSIADVHGVMTFFHDFRAAPPPTHVVAICRGEACQSVGAERLMADVRADLQ
                     EASDVEVHEVFCLGNCALGPSGTVDGRLFGHLTASRVGASLPGRSS"
     gene            12701..14284
                     /locus_tag="PLB86_02790"
     CDS             12701..14284
                     /locus_tag="PLB86_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRC64090.1"
                     /translation="MTRFFVPGDAAAVSVGADEVAAALTGQPGVEVVRTGSRGMLWLE
                     PLVEVDTADGRVGFANVSPGQVGALLADAASVCSRESAASAGSPPEAGHPAYLGPLDE
                     HPWMTRQARLTTARLGVVDPADPDDYARHGGWVGLRRALGMAPEAVVEEVVASGLRGR
                     GGAGFPAGVKWRTVAGAPGPLKFVTINADEGDSGTFADRMLMEGDPFALIEGMAITAW
                     AVGASEGYVFIRSEYPHAIATMRRAVETARTGGWLGPDILGSGLAFDVEVRVGAGAYI
                     CGEETSMLSTIEGRRGEVRAKPPIPALSGLFGRPTVVNNVLTVAAVPSILADGPEAYA
                     AHGVDRSRGTQVFQLAGNVARGGLVEVDFGITLRDLVEGFGGGTRSGRPVRAVQVGGP
                     LGAYVPADRLDVPLAYEAMADAGFMLGHGGVVVFDDTVDMGRMARFAMEFCAKESCGK
                     CTPCRIGSQRGVETIDRLVAGGTESDRRANLALLQDLCTTMERGSLCAMGGLTPMPVR
                     SALTHFPEDFGLSADGEPR"
     gene            14281..17283
                     /gene="fdhF"
                     /locus_tag="PLB86_02795"
     CDS             14281..17283
                     /gene="fdhF"
                     /locus_tag="PLB86_02795"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRC64091.1"
                     /translation="MTLAPERDLGTPAVVGEPTATVTIDGIPVQVAPGTSVLRAASLA
                     GVEIPRLCATDRLEAFGSCRLCLVEVDGVRGTPASCTTLCSDGMSVATDTPMVRQLRH
                     GVMELYLSDHPADCAGCERGSCDLGRLAADAGVAEVRYGLGRSHADATTDVSNPYFAF
                     DPAACIVCSRCVRACGEVQGTFALSVAGRGFDSQISPSGTDFLASECVSCGACVQACP
                     TSALEEKSLVSLGMPSRAVVTTCAYCGVGCSFRAEVRDDPEGTTLVRMVPWKDGGANE
                     GHSCVKGRFAYGYAAHRDRVLEPMVRDHIDDPWQVVSWDTAIERVATGFRAIQERYGF
                     DAIGGISSSRCTNEEVYVVQKMVRAAFGNNNVDTCARVCHSPTGYGLGQAFGTSAGTQ
                     DFESVDQADVILVIGANPTDAHPVFGSRIKRRLRNGAALIVADPRRIELVRSPHVEAA
                     YHLPVQPGTNVAFVNAMAHVIVTEGLADGRFLAERCEDVTDYLAFIARPEHSPEATEE
                     VTGIPAPDLRAAARRYAAGPNSAIYYGLGVTEHSQGSTMVMGLANLAMLTGMLGRDGV
                     GVNPLRGQNNVQGSCDMGSFPHELPGYRHVSGAEVRGIYERLWGVPLLAEPGLRIPNM
                     FDAALGGTFRGLYVQGEDIAQSDPNTRHVEAALRALDLVVVQDLFLNETARFAHVFLP
                     GTSFLEKDGTFTNAERRINRVRPVIANRVGKAEWEVVCDIATAMGYPMGYAAAADIMA
                     EIAATTPTFAGVSFERLDAVGSLQWPVPDVESPGTPIMHVDSFARGRGRLVETVYVPT
                     TERTTRKFPLVLTTGRILSQYNVGAQTRRTENSTWHAADVLEIHPADAEVRGIHSGDL
                     VGLASRVGATTLRALVSDRMPPGVVYTTFHHPVTGANVVTTDNSDWATNCPEYKVTAV
                     EVTVATALDTPAPGHIVATSILSRADDIARQFAGLSPAEGAAAVATHLTRFWEPRMLA
                     ELASRVRSGDPVDPLVVSALAEVESAAH"
     gene            complement(17304..18011)
                     /locus_tag="PLB86_02800"
     CDS             complement(17304..18011)
                     /locus_tag="PLB86_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64092.1"
                     /translation="MASLGSITRVTVPHPWLLAPFEGPRATAEALSLLPSAPWLLATS
                     AVAGHPVLAVPGLSGGNAWTVVLRRFLAIKGYAVHTPDKGTMRGAHATVVARLADRVS
                     ELASEAGQPVSIIGWSVGGAFTRQVALAQPESVRSLITLGAPLSGSWYPEGSAAGGPL
                     PVPTTAIYSRTDGTFDWRRCVQRPGPMTENVEIVSSHLGMATNPMTVHVVADRLGQAD
                     GRWRPYHSAWHLGEDDR"
     gene            complement(18040..18912)
                     /locus_tag="PLB86_02805"
     CDS             complement(18040..18912)
                     /locus_tag="PLB86_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRC64093.1"
                     /translation="MPRAQSAGGIPRARRGRLRPPPHGDPVARQTGGVTVDDLGYAVP
                     LTSPIERVVSLVPSLTEAFAVSAPGLLVGATDWCVHPVSLDVVRVRGTKNPDVPRIAT
                     LAPDLVVASMEENREADVTALRELGIPVWVTDIRTVDGALTSIERLLEIIEAPHRGWL
                     AQAREAWRDPVQVAGDSRLRAVVAIWRRPWMFLGSDTYAGDVLRRLGVDNVLGHSPWR
                     YPRVDFDELPAHDLVVLPDEPYRFTPDDGPEAFPGAATALVDGQALTWYGPRMVQAPT
                     LLAEQLGMSTTDAR"
     gene            complement(18926..19702)
                     /locus_tag="PLB86_02810"
     CDS             complement(18926..19702)
                     /locus_tag="PLB86_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC64094.1"
                     /translation="MSIHEGSWVPGTPCWVDISVPDLARSQAFYRGVLGWEFTPGSEE
                     FGGYTTALVNGRGAAGMSPPMPGADPAPTFWTVYLATADSAASEAAITAAGGQTIFPS
                     MQLADLGRMGVYADSTGAVFGTWESGSHTGYDVANEPGAVGWCEGMVGDFEAGKAFYA
                     AAFGFTYTDMSADGMSYAMFSVPGGEQPAGGLGLETERPPYWSVTFEVDGTDAAVARV
                     LENGGGVILEPFDFEYGRLAVVTGPDGEPFGIITSAPMPV"
     gene            complement(19765..20367)
                     /locus_tag="PLB86_02815"
     CDS             complement(19765..20367)
                     /locus_tag="PLB86_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64095.1"
                     /translation="MTVFAIGGGAGVGKTTAARALAARLGAGWLQLDTVWLALLEGAE
                     PGSARRDALDVRSAVRGAVRAGPVDPDALLARQIAAGGLVEEVLPTVLAFEAAAHRHL
                     VIDGSWFTPAGVALLAADLPVRAAYLHEADPVAVRRAMDTRRAGAHPRPWHDAMAHLA
                     WTYGQWVAEQAQAAGQPVLPARPFATALDRLGVALGCAAD"
     gene            complement(20381..21778)
                     /gene="lpdA"
                     /locus_tag="PLB86_02820"
     CDS             complement(20381..21778)
                     /gene="lpdA"
                     /locus_tag="PLB86_02820"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRC64096.1"
                     /translation="MADFDVVVLGAGPGGYVAAIRAAQLGLKAAVVELKYWGGVCLNV
                     GCIPSKALLKNAELAHVLTHEKAKYGIEGDATMSYGPTHARSRQVSAGIVKGVHFLMK
                     KNKITEIDGWGTLTSPTSMDVKAADGSVRSITFDNLIIATGSVTRMIPGVQVSANVVT
                     YEEQILDPNLPGSIIIAGSGAIGVEFAYVMRNFGVDVTIVEFLDRMVPTEDEDVSKEL
                     AKHYKKLGVKVMLGTKVEAVEDTGSGVRVTVSPAAGGESTVLEADRLLSAIGFAPRVT
                     GFGLEATGVALTDRGAIAIDGRGRTNVPGVYAIGDVTAKMMLAHVAEAMGIVAAETIA
                     GAETMEIDFAFIPRATYCQPQIGSMGLSEKQATDKGYAVKTATFPFAANGKAQGLGDA
                     VGFVKIVADAEHNEILGAHMIGPDVTELLPVLNAAQTWDLTADELSRVVFAHPTLGEA
                     VKEALHGIAGHMINF"
     gene            complement(21788..22300)
                     /locus_tag="PLB86_02825"
     CDS             complement(21788..22300)
                     /locus_tag="PLB86_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRC64097.1"
                     /translation="MILPFGPHTPSIAAGAWVAPTAMVIGDVVIEEGASIWYGAVIRA
                     DRARIRIGARSNVQDNAVFHADPGAPVDLGCDVTVGHGAIVHGATVGDGSLIGMGATL
                     LNHSVVGGGSLVAAAALVREGQVIPPGSMVAGVPAKVLGPVPAGFDVHVSGSVATYET
                     LRDEHREATP"
     assembly_gap    22327..22426
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22430..23671)
                     /locus_tag="PLB86_02830"
     CDS             complement(22430..23671)
                     /locus_tag="PLB86_02830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC64098.1"
                     /translation="MAVEPALRRPEVRRVITSHAFAAVGMGLPWPVLLLAVEQSTSSQ
                     LLLGLAGAARLAPYVALSWLSGRLADRRERALIVRISLWARAVALLVAAAALAASNPL
                     VAVVAATGAIVAGTPAYPALAAGMPRLAGAATERATSALVTVEVASFVVGPAVGGLFV
                     GRMPPAWVPLIGAIFVGVAIVAFGRTAMPAPEASAIDVAEGGVWSLLRRDPCARQALA
                     VVAVVNALLSVLGLLLLQLAHEVWRSGDEGFGLATALLGFGALGAPVVAHSRTMRSGR
                     AALLTLAGAAAVFALLPGGPASFGALAVIGAVAVCCENVATSVLQATVPDELRASVLG
                     LGDTVMVGAALLAAVATPWLGVHLGPASLLLLVAAGTAMLALAWSAGRQGPDSVATLP
                     GGSGEELPVQHEDVGVRVPGV"
     gene            complement(23772..24065)
                     /locus_tag="PLB86_02835"
     CDS             complement(23772..24065)
                     /locus_tag="PLB86_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64099.1"
                     /translation="MALPWWTLFVLAAAACSWISVTVLARNNPGERLSWFRSPTVAPL
                     SQLWFLAASVACAWLGGSYAADTALGGWAYPIAALAVLVPWALVRAGHNRHVG"
     gene            complement(24073..24846)
                     /locus_tag="PLB86_02840"
     CDS             complement(24073..24846)
                     /locus_tag="PLB86_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64100.1"
                     /translation="MTRLRGMVLIGGLVPLLSGCLVVSALNASNGPATAAPTSIATFD
                     PFATGAVPKATGATPSPSPSPTPSTSSSPSPKPSASPPPPALPAGAKWATVDSELVRF
                     AVASAWVEIVPATMTTTASIPADVKAIAKALGISARELIDRFDDIEIAYVAAPVNRYA
                     TNLSATVADVGSLPDDDWVKTTYSEGKVKVSGSERLATRVGDALRINGTVTVQGASIA
                     LTTFIVDVGRHCIVFEVAARKRADVDSAIARLASTLHEI"
BASE COUNT         3709 a         8838 c         8468 g         3550 t
ORIGIN      
        1 accttcgacc cgagcaactc ctcgatcgcc gcacgcgccg tcgacccgac atcgcgcagc
       61 cgcgcgccac cgcgcccgat gacgatggcc ttctggctgt cgcgctcgac aaagaccgac
      121 acccgcacgt cgagcatcgg cgcgtggccc tcagggacct cccgcggcac gatctcctcg
      181 acgacgaccg cgagcgagtg cggcaactcg tcacggacgc cctccaacgc agcctcacgg
      241 accaactcgg cgatcatcac gtcgtcgggc tcgtcggtcc gcgcctccga cggatacagc
      301 ggcggcgaca gcggcaacag cccgaccaac accgtgcgca cctcgtcgac ctgatcgccg
      361 cgggtcgccg agcagggcac gatggcgtcc cattcgccca aggcgtcgat cgccacgagg
      421 tgggtgagca ggcgctcccg cgacaccagg tcgctcttgg tcgccagcgc caccacgggt
      481 ttgcgcttga cccgccgcag ctccgcaagc tcggccgcga tgaactggtc acccggcccg
      541 acccgctgat cggccggcag gcagaagccc acgacgtcga cgtccaacag tgcctcgcgg
      601 accaggtcgt tgagccgctc ccccagcagg gtccgcggcc ggtgcagacc cggggtgtca
      661 atgaggatga gctggctgtc ggccgtggtc gagatcccgc ggatcgtatg ccgcgtcgtc
      721 tgcggcttgg acgacgtgat cgccaccttc tgcccgacca aggcgttggt cagcgtcgac
      781 ttgcccgcat tgggtcgccc cacgaggcag gcaaaaccgg ctcggtatgc cgcgtcctca
      841 ccttggtcag tcatcgtcgc ctcccacacg gtcagggtca ccagccagcc ggcgcaccag
      901 cggctcggac tcggtctcgc ccaatggcac caaggggcgc acgagcaccg tggcgatccg
      961 gtgccggtga gccatcctct cagccgtgat cgacaacccc gcgatcccag catgggcgcc
     1021 ggcgaacggc acccgcccca acgccttcgt gagcagcccg ccgatgctgt cgacctcgtc
     1081 ttcgtcgatg tccaggtcga acaactccgc aacgtcgtcg atgtgcatcg cggcgggcaa
     1141 ccggtagccc ccgtccccga tcggttccac ccctggctcg tcccggtcgt attcgtcggc
     1201 gatctccccc acgatctcct caaggatgtc ctcgatggtg accaggcccg cagtgccgcc
     1261 gtattcgtcg acgacgaccg cgaaatgcac ctggtcacgc tgcatctcac gcagcaggtc
     1321 atcgatcggt ttggactcgg ggatgaacga catcggccgc atcaggctcg aacaggccac
     1381 cgcgcccgcc tccgggtcgg cggtgatccg cgcgacgacg tccttgaggt agatgaggcc
     1441 taggacgtcg tcggagtcat ccccgatgac cggcagccgg gagaaaccgc tgcgcaggaa
     1501 gaccgccgtc gccttgcgca acgggtcgtc accttggacg gtcaccatgt cggggcgggg
     1561 caccatgacc tcgcgtgcca ccgtgtcacc caactcgaag accgaacgga tcatctcccg
     1621 ctcgtcgtcc tcgatgaccg atgagtcgga tgcctggtcg agcagatctc gcaactccga
     1681 ctcggtctgg aacggcccgt cgcgatagcc cttccccggc gtcaccgcat taccgagggc
     1741 gatcagccca tgcgagacgg gcccgagcac gcggcgcaac gctcgcgtca ccggcgcggc
     1801 ggcaagagcg gcccgctcgt agttttgtcg accgagcgtc cgcggcgaca caccgacgag
     1861 gacgaaggac acgagggcca tgacgaggat cgagatcgcc agcgccgtcc ccaacgaatc
     1921 ggtgagggcg atgacggcga cggtgatgag cactgccgtg gtcgcctcag cgacaacccg
     1981 caagaaggcc aagactgcca ggtagggggc gggttgctcg accacggcca cgagggcagc
     2041 agcgccgacc cgaccgtcgt cagccaactc ctcggcacgg acccgggtca tgcgctgcaa
     2101 ggcggcctcc gccgcggaca agcagaaggc caacaccgtg gccaacacgg tgacgacgac
     2161 gacttgggcc agcaccacgc cgctcatcgc tcaccgactc caatcatcgt cggccgcggc
     2221 cggcgagata ggtcaggagc aactgccgct gcaactcgaa catctcgtgc ttctcgtcgg
     2281 ggtcggcgtg atcgaagccc aacaggtgca ggagaccatg ggtcgtcagc aggagcagct
     2341 cctcgaccgg agcatgcccg gcttggcggg cctgctcctc ggccaccgcg ggacagagga
     2401 cgatgtcgcc gaggacgcct tcttgcaggt cgacgccgtc ccgtcccggg cgcaactcgt
     2461 ccatggggaa gctcatgacg tcggtcggac ccggcaggtc catccatttc tcgtgcagca
     2521 ccgtcatcgc cgcctcgtcg accaggctga tggccaggtc ggcgtccggg tgcaccttca
     2581 tccggtccat gacatgacgg gcgcacgcca cgagttccag ctcgtcgacg gcatacccgc
     2641 actcgttgag gacgtcgacg ctcacgggcg gttcgctcct ccggccaggt gccgtggggt
     2701 atgccgcccg tggtcctccg cttcgcgcgc ggcctgctcc ttcgcctcgg acgcgccgta
     2761 ggcgtcgacg atggcgctga ccaacttgtg gcgcacgaca tcggcggagg tgagctcggt
     2821 gaagtagatg tcgtcgacgt cggcgaggat gtcgcgcacg tgggcgagcc ccgagcgggt
     2881 gccgcctggc aggtcgatct gggtgatgtc gcccgtgaca accatcttcg agccgaaccc
     2941 gagccgggtg aggaacatct tcatctgctc ggccgaggtg ttctgagcct catcgaggat
     3001 gacgaaggcg tcgttgagcg agcgaccgcg catgaaggcc agcggcgcga cctcgatggt
     3061 gcccgaggcc atcagccgcg ggatcgtttc agggtcgacc atgtcgtgca gcgcgtcgta
     3121 gagcgggcgc agataggggt cgatcttgtc gctcagcgtc ccgggcagga agcccaaccg
     3181 ctcgcccgct tcgaccgcgg gccgggtgag gatgatccgg ttgacccgct tgctctgcag
     3241 cgcagcgacc gccttggcca tggccagata ggtctttccg gtgccggctg ggccgatccc
     3301 gaagacgatc gtgtgcatgt cgatcgcgtc gacataccgc ttctgcccca gggttttcgg
     3361 ccggatcgtc cgaccgcggt tgctgatgat gttgagggtc agcacgtcgg ccggacgctc
     3421 cgcggtgcgg gcccgcaaca tcgcgatcgc tcgctcgacc gcgtcgcgtg tcaggggttg
     3481 cccagcgtcg atgacgacga gcaactcgtc gatcaaccgc tcgacgacgg ccacgtcgac
     3541 ccccggcccg cgcaacgaca actcgttgcc gcggacatgg acgtcgaccg tcgggaaggc
     3601 ccgctcgatc gtgtccaaca gctcgtctcg gggtccgagc agcgtcacca tcgacacggt
     3661 cggagggatg gtgatggcgt gagtgggcag cgccgcggcg acggaggccg agggcatgcc
     3721 gcgcgggtgc ggctcggtca tggcgcagac cagtctatga ggcgcgcggc ataccgatcg
     3781 acgaagattc ccaccagcgt gcggttcctc accccggagg ccactgcatc ggccggcccc
     3841 cgagcacgtg cacgtgggcg tggaagacga tctggccgcc ctcggagccg gtgttcgtca
     3901 ccatccggta gccccgaccc aggccttcct ggtcagcgac tcgcgccgcc aactcgaaca
     3961 ggtcggcgag ctcccgcggc gcatcggcca actcgccgac gttctggacg tgccgccgcg
     4021 ggatgacgag cacatgggtg ggtgcctgag ggtcgatgtc acggaaggcg agcgagtgtt
     4081 cgcttcgggc gacgacggtc gccgggatcc gttcggcgac gatcttgcag aacaggcagg
     4141 tgggcgcgct catgctgcga gactagtcgc gccggcgagg tcaaccgacg tccgcggcca
     4201 gccgtccaga gagcgtgccg acgatgcagc tggaacgccc cggtgcgcgc gggtctgcgc
     4261 gcacacccgg tgccgctgtc ggcgtcacgt ccgccgatga ggcgatcgcc atcctgtatg
     4321 ccgcccactg gcaccctctc gtgcggctgg cctggttcct cgtccacgac caaggcaccg
     4381 cggaggacgt cgtccaggac gcgttcatcg ccacccaccg caactacgac cggatccggg
     4441 acaccggcgc ggcggtcgcc tatctgcgcc gggccgtcgt caacggcgcc cgctccgtgc
     4501 agcggcacga cgttgtcgtc cggcgggaag cgatcgccga ggccggccga gcggacgctg
     4561 ccgaccgggc cacggcggcc agtgcggagg cgcaggccct gcgccatttg ggggacggcg
     4621 ccatgttggc cgccctacga cgcctgcccg accgccaacg agaggtcctc gtgctgcgct
     4681 acttcgccga ctggtccgag gccgccattg ccgacgcctt gggcatcgcc cccggctcgg
     4741 tcaaggcgca cgcccaccgc ggcctggctg ccctgcgggc ccgattggag gcgaccgaat
     4801 gacggcccac ccaccaggag acccgcacgt ggaagcgcgg ctgcgcgatg ccctcaccac
     4861 ggccgccgag cgagtctccc cgactgatcg gctcggtgcg atcaccgaag cggtgtcgac
     4921 ggcacccccg cgagccggca caccccggtg gctgctcccg ctggcggcgt cgttcctcgt
     4981 tctcgcagtg gggctgggcg cattgaccct gcagcagcgg gtcggcagca acgcggtggc
     5041 cgcatccggg acccacacgg ctgtggtgat cgcggccgac gacccgctgc cagcaccctc
     5101 acgggcgctg cccgcgtcgc ccggtgcatc caccccgggc tcgacgagcg cgacccgttt
     5161 cgagtggtcc gcccccgtct attacgcagc accggggacg cccgtcgtgc ggtgggcgct
     5221 ctatcgcgac ttcgtgcgcg ccacgatggg cgatgacggc ctcgatgccc gagtcaccac
     5281 ggccgtcaac ctcgctcttg cacagccaga tgtctggggc caggccatgc cgggccagga
     5341 cggctacctg cgcgcctgga cgcccggcac gcgcgtcacg gccagcgtcg cggcggatcg
     5401 gatcgccctc accctcagcg cacctggtgt cggcgggctc gacgcggacg cgcaacgaat
     5461 ggccgtgcag gcgttggtgt ggaccgccac cgcggcggcc caacgcaacg tcccggtagc
     5521 catcgacgtg gccggcgccg gcccggtggt gggcggcata cctgctgggg tgttccggcg
     5581 accggctgac accacccggg agctggcccc cgtctggatc acgtcaccgg ggcggttcgc
     5641 ctcggtgtgg gactcgcgcg tggtcatcga gggccaggcg tgcacgttcg agggcaccgt
     5701 ccaatgggag ttgcggctcg acggagcgat ggtccgccag ggattcacga ccgcgacggc
     5761 cgggtgcccc gtccgaggga cctggcgggt cgacctcggg gtgttggcgc cgggccgcta
     5821 cacgatccga gcattcgagt cggatgccgc gaccggtggc cccttcgccg agcagtcggt
     5881 ggccttcacg gtccgttgac cctcggctca gcgccagcgc gacatcccgt tgaggaccgc
     5941 cagcgccgcc gggcccgccg tcgaggtccg caacacctcg ggcccgagcc ggaccgcgac
     6001 cacccccacc gccgcgaagg catccaactc ggtctgtgcg atgccgccct cggggccgac
     6061 gacgagcacg atctccccgg tcggcggcag gtcgaggctc gccaacggcg cctgtgcctc
     6121 ctcgtgcagg acgatgctca gagcggcatc gcccaacagc cgcaccacat cggcggtgcg
     6181 cgccagaggc ctgacctgcg ggacctggaa ccggcgggac tgctttgccg ccgccaccac
     6241 gatcgactcc cagcgcgcct ggccccgggc gaccttgtcg gcgcgccact gaacgacgct
     6301 gcgggacgcc tgccacggga cgacggcatc gacgccgagc tcggtcgcgg cctcgatcgc
     6361 ctgctcgtcc cggtcaccct tggccagcgc ctggaccagg acgaacctcg ggtccgcggg
     6421 cgggcgcacc tgcacctcga tgaccctggt gaggagggta tgccgttcga cgccgaccac
     6481 ctcggcgctc acccggaccc cggccccgtc accgacaagc acctcttccc cgatgcgcaa
     6541 ccggtggacc gagaccgcgt ggtggccttc cgagccgccg atcgacaccg tggtgcccgc
     6601 catcgccccg gccagagagc caggtgcgac gaggaacagc ggggcggtca cggcataccg
     6661 gcttcgtcag gcgttggtcg ctgggagttc gtcgtcaggc gttcttgaag gcgtcgcgca
     6721 acttgccgag caggcccttg tcggcaggcg ccatccgccc gatcggacgt tcctcgccac
     6781 gcaactgggc caactgacgc aacaggtcct gctggccctc ggtgagccgg gtcggagtct
     6841 ggacgtcggc atggacgatg aggtcgccgc ggccggtgcc gcgcaggtgg gtcaccccaa
     6901 ggccacgcag cgtcatcgtg tcgcccgcct gggtgcctgg ccgcacgtcg aaggggcggg
     6961 tgccgtcgaa ggtctgcagg tcgatcgtcg tccccagggc cgcggccgtc atcggcaact
     7021 cgacccggca gtgcaggtcg tccccccgcc gcgtgtaggt ggcgtggttc gccacggcga
     7081 cctcgacata caggtcgccc gccgagccgc cgccggtgcc gacctcgcct tcgccggcca
     7141 gttggatgcg ggtgcccgag tcgaccccgg ccggaacctt gagggtgagg gtgcgccggg
     7201 tgcggacccg accctcgccc gagcactcga aacacgggtg gttgatcgtc gagccgtagc
     7261 cctggcaggc ctggcagggc cgagtcgtca tcacctgccc gaggaaggac cgctgcaccg
     7321 tctgcacctc gccgcggccg tggcacagga cgcaggtcga cacgccggtc cctggctggc
     7381 agccgtcacc ctggcaggac gagcatcgga cggcggtctc gatggtgagc tcctcctcag
     7441 agccgaagac cgcgtcgtgc aggtcgatct ggatccggac gagggcgtcc tggccgcggt
     7501 gcgcccgcga gcgcgggccg cgctgggtgg ccgcacctcc gaagaaggcg tccatgacgt
     7561 cgctgaacga gaagccctgc ccgctgaagc cggcgccccc acccgcggcg aaggggtcga
     7621 caccgaggtc gtagttgcgc cgcttgtccg ggtcagagag cacctcgtag gcccgagaca
     7681 ccgtcttgaa ctgttcctcg gcctcggcgc ccgagttgac atcagggtgc aactggcggg
     7741 cgagccgtcg gtaggcacgc ttgatctcct ccgccgtggc ggagcgttcg acgccgaggt
     7801 cctggtagta gtcgctcaca attccttctc ggtgcagtgg ctgtcggtgc tcgatcaggg
     7861 ctcaagcagc tcggagacgt aatgggctac ggcgcggacg gtcgccatgg cgttggggta
     7921 atccatccgg gtggggccga ggatgcccag gcccgcgacg agctcggcac ccgtgccgta
     7981 ggccgccgag acgactgacg ccccggccag tccctggtgt tcgtgctcgt gaccgatggt
     8041 caccgcgacc gcgccgccct ggccctggcg cagttggccg aacagcttga gcagcacgac
     8101 gtgctcctcg agcgcttcga gcaccggtgc gatcgtcagc gggaagtcgg cgccgaaccg
     8161 ggcgaggttg gacgtccccg ccaggacgac ccgctcctcc cgctcctccg cgagggcgtc
     8221 gtccagcgtg cgcagcacga tgcgaaccag atcgcgatcg gccggctcga aacgctcggg
     8281 gagggattgc aggcgggccg cagcgtccgg gaaggaccgt ccggcggcct cgccgagcac
     8341 ctcggagcgg atccgggaca cgagggcggc gccggtctcg tcgtccaggc ggcagtgggc
     8401 gtcgacgacg cgctgttcga tccgcccggt gttggcgatg aggatgacca tgagccgatc
     8461 acgggccatc ggcacgagct cgatgtgtcg caccgccgac cgggtgagcg acgggtattg
     8521 gacgacggcg acctggtggg tgagcgacgc gagcaggcgt acggtccggt ccacgacgtc
     8581 gtcaagatcg acggcaccct gcaagaagtg gctgatcgct gaccgttcgg caccggacat
     8641 cggcttgacc gcactgagcc ggtcgacgaa cacccgatag cccgcgtcgg tcggcacccg
     8701 accggccgac gtgtgcggcg catggatgag cccctcctcc tcgaggatgg ccatgtcatt
     8761 gcgcacggtc gccgctgaaa caccgaagtg gtggcgttcg acgaggctct tggaccccac
     8821 cggctcggac gtgcggacat agtcctggac gatggctcgc aacaccgaca gccgccgctc
     8881 gtcgctcatc gccctccctc gtcccgctcg tcggtcctac gcccacccgt ctcggcagcg
     8941 cactggcact ctcaccaggt gagtgccaag tctacgtggg tctggcggcg cgtccacccg
     9001 ctcggcgcgc ggacgcggca taccctgccg ggatggatcg ctacggtcgc gacgtcctcg
     9061 ccgacgcagc acgtatgccg cgtcgccccg tcgccgctcc cgtcgccgct gaaccgggcc
     9121 tggtcgtcga ggatgtcgag accggctggt gcggcgcggt cgtgcgcgtg gagaaggccg
     9181 gtggcatgca tgtcgtccac ctcgaggacc gccgcggccg caccaagggc ttcccgctcg
     9241 ggccgggttt cctccttgaa gggcgtcccg tggtcctcac gccgccggtg gcggccgacc
     9301 gcgcgaagtt ggaggcggtc cgcaaggcgg cggcgcgcac ggccagcggt tcggtggccg
     9361 tggccggcca acccgcccgg gtcgccgtcg gctcccggat ctgggtcgag ggacgtcacg
     9421 atgccgagct cgtcgagaag gtctggggtg ccgacctgcg ggtcgaaggc gtcgtcgtcg
     9481 agcagttgga cggcatcgac gacctcgtgg ccagcgtccg gtccttcgcc ccgggtccgg
     9541 gacggcggct cgggatcctt gtcgaccacc tcgttccggg caccaaggag tcggccatcg
     9601 cggcggcagt gcgggcgctc cccgctgcca cgcaagggca tgccctgctg ctcggccacc
     9661 cgtatgtcga cgtctggcag tcggtcaaac cggcccggtt ggggtggacc caatggcccg
     9721 tcatcccgcg cggcaggtcc tggaaggaag gaatctgcca ggcgctgggc tggccctgcg
     9781 acactcccgc cgaccttgcc gtcgcctgga aacgcatcct tggcacggtg ggctcgtatg
     9841 ctgatctgga gccaaccctg ctcgcgtcgg tcgaagagct catcgacttc gtcaccgcac
     9901 caggcgcctg attcaggcgt ccgacaccgg aggtaccaat gtcagaccag cccgccaacg
     9961 acccgacccg ccccgtcgac gttccgccac cgccgccgcc cgcctggagc ggctcggccc
    10021 ccgaggcacc cgccgcgccg gtcccggcac cccccgccta ccaggctccg cccgccgcct
    10081 accagacccc ggcagcgccg cccccgcccg cctacccgcc gcaggggtat gccgcccccg
    10141 tcgccggagt cgtgccgctc agcccagccg agtcacgcca gtgggggatg ctctcgcacg
    10201 tcggcggcat catcctcagc gtcgtcggcc cgctcatcgt catgctcgtg ttcggccccc
    10261 gggacgcctt caccaaggac cagtccaccg aggcactcaa cttccagatc accctgttga
    10321 tcggttacat cgtcgccacg atcatcacgt tcatcatcat catcccgctg ggcctcgtcg
    10381 tctggatcgc cgggctggtc ttcgggatca tgggcgcgat ggccgccaac aagggcgaga
    10441 cctaccgcta cccgttcaac ctgcgcctca tcaagtagtc gcaggccgct gacagtttcc
    10501 gcgtgcgccg gttggtgggt cagcccacca gccggcgcac cactgtgtcg gccaacaatc
    10561 ggccggtcag ggtgaggcgg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnttgacgtt ccaccaccgc acgccgccga cgtggctatg cgctccggga ccggcgcccc
    10741 accagttgcc ccccgcccag tagccgatgt tgtgccgaca gcgctggtcc ggtgtgcggg
    10801 cccagttgct cacctcgtac cactgcatcc ccgcggcggc gaggaccgca tcggccacct
    10861 cgtacttgct cgcctcgtca tcgtcgtcgg gcatcgcgac ctcgccgcga cgaacctgcc
    10921 cggccaggcg ggtgccctcc tcgacgacca gcgcgtaggc cgacaggtgg tcgggcgcca
    10981 actcaagcgc cgcgtcgagc gagcgccgcc aatcggccac gctctccccc ggcgtgccgt
    11041 agatgagatc gaggctgacg gccatccccg ctgcgcggac cgccgcgacg gcgcgcgcca
    11101 cggcggcggg gtcgtgggtg cggtcgaggg tcgcgagcac gtgcggcacg gccgactgca
    11161 tccccaggct cacccgggtg aaccccgccg cggcgagggc cgccatcccc tccggcgtca
    11221 cggtgtcggg gttcgcctcg gtcgtcacct cggcatcggg ggcgagtccc caccgatccc
    11281 gtatgccgtg cagcaccttc gcgaggtcgg ccgccggcaa gagggtgggc gtgccaccac
    11341 cgacgaacac ggtctcgacc tggcgcgggc ctggcccgct gccgccgtcg gtgaggacgt
    11401 cgacggccag gtccaattcc cgcagcacct ggtcggcata gccgctgacc tcggccgctc
    11461 cgtcggcacc caactcaggg atcgcgtagg tgttgaaatc gcagtagcca cagcgcacgc
    11521 ggcagaaggg gacgtggacg tagacgccaa acggcgtgga cgcgatcgcg tcaagagcgc
    11581 cggccgggag gcgacccgta gcgggggcgg ggtccccgtc ggggagggcg gacggcatac
    11641 ccgaatcctc tcagcccggg cggtcgaatc atgcgcggtc gaaacgcgcg cgggtcgaat
    11701 catgcgcggg tcgaatcgtg gacgggagtc ttgcgcctcg ggaccgcgac gacgctgcgg
    11761 cggcgtgtcg caccgacacg ccgtgggtcc cgcgaaagcc ttcagaagtc tcgcccaacc
    11821 ccttgtgaac cggcggtgac gggactagaa atggggatac gccaacggtt cgccggaggc
    11881 cagcaaggcg ccaccatcgg cccaaaactc tttcgggagt ggcggattcg aggtgctgag
    11941 cgaagggtcg gacctcggtc cggccacgct gcgagcgcag gttcgcggca gcccgacctt
    12001 cgggtcggag gcgtcgagct tcgcgcccga cgcacggtgg tcggcttcgg ccgagcgcca
    12061 ggaagggccc cggaacctca tacgttccgg ggcccttctt cctgcccnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnctt cctagactgc ggacactcac atcaacggcg
    12241 aggagggtgg cgcacgcatg gtggagatga cccgcgtcga cgcggtccgc gagatcgctg
    12301 cggggtatgc cgcgcgccga ggccccctgc tgcccgtgct gcacgcggtc caggaccggt
    12361 tcggagccat cgagcgtggc gacctggttg ccgtcgccga cgtcctcaac ctctcgatcg
    12421 ccgatgtcca cggcgtcatg accttcttcc acgacttccg cgccgctccc ccgccgacgc
    12481 acgtcgtcgc gatctgtcgc ggcgaggcct gccagtcggt cggcgccgag cgcctcatgg
    12541 ccgacgtccg cgccgacctg caggaggcat cggacgtcga ggtgcacgag gtcttctgtc
    12601 tgggcaactg cgctctcggc ccgagcggca ccgtcgacgg tcgcctgttc gggcacctca
    12661 ccgcttcgag ggtgggcgcg tcgctgcccg ggcggtcgtc atgacccggt tcttcgtccc
    12721 cggtgacgcc gctgccgtgt cggtcggcgc ggacgaggtc gctgcggccc tgaccgggca
    12781 gcccggcgtt gaagtcgtcc gcaccgggtc tcgcgggatg ctctggctgg aacccctcgt
    12841 cgaggtcgat acggccgacg gtcgggtggg cttcgccaac gtctcaccgg gccaggtcgg
    12901 cgcgctgctc gccgacgcgg cgtcggtgtg ttcgcgcgag tcggctgctt cggccggatc
    12961 gcccccggaa gccggccacc cggcatacct cggccctctg gacgagcacc cgtggatgac
    13021 ccgacaggct cgcctgacca cggcgcgctt gggcgtcgtc gacccggccg atcccgacga
    13081 ctacgcgcgg cacggcgggt gggtcgggct gcggcgggcg ctgggcatgg cccccgaggc
    13141 cgttgtcgag gaggtcgtcg cgtcggggct gcgtggccgg ggcggtgcgg gcttccccgc
    13201 cggggtgaag tggcggaccg ttgcgggcgc gcccgggccg ctgaagttcg tcaccatcaa
    13261 cgccgacgag ggcgactcgg gcacgttcgc cgaccggatg ctcatggaag gagacccctt
    13321 cgcgctgatc gagggcatgg cgatcaccgc ctgggccgtc ggtgcgagcg agggctacgt
    13381 cttcatccgt tcggaatacc cgcacgccat cgccacgatg cgccgagccg tcgagacggc
    13441 gcgaacgggt gggtggctcg ggccggacat tctcgggtca gggttggcgt tcgacgtgga
    13501 ggtgcgggtc ggcgccggcg cgtatatctg cggcgaggag acctcgatgc tctccaccat
    13561 cgaaggtcgc cgcggcgagg tgcgcgccaa gccaccgatc cccgcgctgt cggggttgtt
    13621 cgggcgaccg accgtcgtca acaacgtcct cacggttgcg gcggtcccgt ccatcctcgc
    13681 cgacggtccc gaggcgtatg ccgcccacgg cgtcgaccgc tcgcgcggca cccaggtgtt
    13741 ccaactggcc ggcaacgtcg ctcgtggcgg cctggtcgag gtcgacttcg ggatcaccct
    13801 gcgcgatctc gtcgagggtt tcggcggcgg cacccgctcg ggacgtccgg tccgagccgt
    13861 ccaggtgggc ggaccgctgg gggcttacgt gcccgccgat cgactggacg tgccgctggc
    13921 ctacgaggcg atggccgatg cgggtttcat gctggggcac ggcggggtgg tcgtcttcga
    13981 cgacaccgtc gacatgggcc ggatggcccg cttcgctatg gagttctgcg ccaaggagtc
    14041 gtgcggcaag tgcacgccct gtcggatcgg ctcgcagcgc ggcgtcgaga cgatcgaccg
    14101 gctcgtcgcc gggggaaccg agtcggatcg ccgggcgaac ctggctctcc tgcaagacct
    14161 gtgcacgacc atggagcgcg gctccctgtg tgcgatgggc gggctgacgc cgatgccggt
    14221 gcgcagcgcc ctcacccatt tccccgagga tttcgggctc agtgcggacg gagagccccg
    14281 atgaccctcg cccccgaacg tgacctcggc acaccggcgg tggtcggtga gccgaccgcg
    14341 acggtgacga tcgacggcat acccgtgcag gtcgcccccg gcacctcggt gctgcgtgct
    14401 gcttccctcg ccggggtcga gatcccccgc ttgtgcgcca ccgatcggct ggaggccttc
    14461 gggtcgtgcc ggctctgcct cgtcgaggtc gacggcgttc gtggcactcc cgcgtcgtgc
    14521 accaccctgt gctccgacgg gatgagcgtg gcgaccgaca ccccgatggt ccggcagctg
    14581 cggcacgggg tgatggagct ctacctgtcc gaccacccgg ccgactgcgc aggctgcgag
    14641 cgcggctcct gcgacctggg gcggctggcg gcggacgccg gcgttgccga ggtccgctac
    14701 ggcctcgggc gctcccacgc cgacgcgacc accgacgtga gcaaccccta cttcgcgttc
    14761 gacccggcgg cctgcatcgt ctgctcgcgc tgcgtgcggg cctgcggtga ggttcaaggc
    14821 accttcgcgc tgagcgtcgc aggtcgtggc ttcgactcgc agatcagccc tagcggcacg
    14881 gatttccttg cctccgagtg cgtttcatgt ggcgcctgcg tgcaggcctg ccccacatcc
    14941 gccctggagg agaagtcgct cgtctcactc gggatgccct cgcgggccgt ggtgaccacg
    15001 tgcgcctatt gcggtgtcgg gtgttccttc cgcgccgagg tgcgcgatga ccccgagggg
    15061 acgacgctcg tgcggatggt cccgtggaag gacggcgggg ccaacgaggg tcactcgtgc
    15121 gtcaagggcc ggttcgccta tgggtatgcc gcccaccgcg accgggtgct cgagccgatg
    15181 gtccgcgacc acatcgacga cccctggcag gtcgtgtcct gggacacggc gatcgagcgg
    15241 gtggccaccg gtttccgggc gatccaggag cgctacggct tcgacgccat cgggggcatc
    15301 tcctcgtcgc ggtgcaccaa cgaggaggtc tacgtcgtcc agaagatggt gcgggccgcc
    15361 ttcggcaaca acaacgtcga cacctgcgct cgggtctgcc actcccccac cgggtatggg
    15421 ctcggccagg cgttcggcac gtcggccggc acccaggact tcgagtcggt cgaccaggcc
    15481 gacgtcatcc tcgtcatcgg cgccaacccc accgatgccc acccggtctt cggctcgcgg
    15541 atcaagcggc gcctgcgcaa cggagcggcg ctcatcgtcg cggacccccg acggatcgag
    15601 ttggtccgct ctccgcatgt cgaggcggca taccacctgc ccgtccagcc gggcacgaac
    15661 gtcgccttcg tcaacgcgat ggcccacgtc atcgtcaccg aggggctggc cgacgggcgg
    15721 ttcctcgccg agcggtgtga ggacgtcacc gactacctcg ccttcatcgc ccgccccgag
    15781 cactcgccgg aggcaacgga ggaggtcacc ggcataccgg cgcccgatct gcgcgccgcc
    15841 gcccgcaggt atgccgcggg ccccaactcc gcgatctact acggcctcgg ggtgaccgag
    15901 cacagccagg gctcgacgat ggtcatgggc ctggccaacc tcgcgatgct caccgggatg
    15961 ctcggccgcg acggggtcgg ggtcaacccg ctgcgcggtc agaacaacgt ccaggggtcg
    16021 tgcgacatgg ggtccttccc ccacgagttg cccggctatc ggcacgtgtc cggggccgag
    16081 gtccgaggga tctacgaacg gctgtggggt gttccgctat tggccgagcc gggcctgcgg
    16141 atcccgaaca tgttcgatgc ggccctcggc ggaacgttcc gagggctcta tgtccagggc
    16201 gaagacatcg cccagtccga ccccaacacc cgccatgtcg aagccgcgct gcgggccctc
    16261 gacctggtcg tcgtccagga cctcttcctc aacgagacgg cgcgcttcgc ccacgtcttc
    16321 ctgcccggaa cgtcgttcct cgagaaggac gggacgttca ccaacgccga acgccggatc
    16381 aaccgggtcc gccccgtcat cgcgaaccgg gtcggcaagg ccgagtggga ggtcgtgtgc
    16441 gacatcgcga cggccatggg ctaccccatg gggtatgccg ccgccgcgga catcatggcg
    16501 gagattgccg cgacgacgcc gaccttcgca ggcgtctcgt tcgagcgcct cgacgccgtc
    16561 gggtcactgc agtggcccgt ccccgacgtc gagtcgccag ggaccccgat catgcacgtc
    16621 gactcgttcg cccgaggtcg gggccggctc gtcgagaccg tctacgtccc cacgaccgag
    16681 cgcaccaccc gcaagttccc gctcgtgctg accaccggcc ggatcctcag ccaatacaac
    16741 gtcggagccc agacccgtcg caccgagaac tcgacctggc atgcggccga cgtcctggag
    16801 atccatccgg ccgacgccga ggtgcggggc atccactccg gtgatctcgt cgggctggcc
    16861 tcccgcgtcg gcgcgacgac gctgcgggcg ctcgtgtccg accggatgcc tcccggcgtg
    16921 gtctacacga ccttccacca cccggtgacc ggcgcgaacg tcgtgacgac ggacaactcc
    16981 gactgggcga cgaactgccc cgaatacaag gtgacggcgg tcgaggtgac cgtggcgacc
    17041 gcgttggaca caccggcgcc tgggcacatc gtggccacga gcatcctctc ccgggccgac
    17101 gacatcgccc ggcagttcgc ggggctctcg ccggccgaag gcgcggccgc cgtggccacc
    17161 cacctcacca ggttctggga gccgcggatg ctcgccgagt tggcctcgcg ggtgcggtcc
    17221 ggcgacccgg tcgaccccct cgtcgtcagc gctcttgccg aggtggagtc ggccgcgcac
    17281 tgagccggac gttggtcacc ggctcagcgg tcgtcctcgc ccaagtgcca ggccgagtgg
    17341 tagggacgcc agcgtccgtc ggcttgaccg aggcggtcgg cgacaacgtg gacggtcatc
    17401 gggttggtcg ccatccccag gtggctggag acgatctcga cgttctcggt catcgggccc
    17461 ggccgctgca cacatcggcg ccagtcgaag gtgccgtcgg tgcgtgaata gatcgcggtg
    17521 gtcggcaccg gcagcgggcc acccgcagcc gacccctcgg gataccagga ccccgagagc
    17581 ggcgcgccca gggtgatgag gctgcgcacc gactccggct gggcgagtgc cacctgccgg
    17641 gtgaacgcac cgccgacgct ccacccgatg atgctcaccg gctggcctgc ctcactcgcc
    17701 aactcgctca cccggtcggc gaggcgagca accacggtgg cgtgagcgcc gcgcatggtg
    17761 cccttgtccg gggtgtgcac ggcatacccc ttgatcgcga ggaacctgcg caggaccacg
    17821 gtccacgcgt tgccccccga cagcccggga acggcgagaa ctggatgacc cgccacggcg
    17881 ctcgtcgcca gcagccaggg cgccgaaggc agcagcgaca gcgcttcggc ggtcgcacgg
    17941 ggcccctcga acggggcgag gagccacgga tgtggcacgg tcacccgggt gatgcttccc
    18001 aacgacgcca tgccgccacc tccggcccga ccctaaccgc tagcgggcgt cggtggtgga
    18061 catgcccaac tgttcggcca acagggtcgg cgcctggacc atgcgggggc cgtaccacgt
    18121 gagggcctga ccgtcgacaa gcgcagtcgc ggcaccgggg aaggcctccg gaccgtcgtc
    18181 cggcgtgaag cggtagggct cgtcgggcag gacgacgagg tcgtgggccg gcaactcgtc
    18241 gaagtccacg cgcgggtaac gccagggact gtgcccaagg acgttgtcga cgcccaatcg
    18301 acgcaggacg tcgccggcgt aggtgtcgct gccgaggaac atccacggcc gtcgccagat
    18361 cgctacgacc gcccgcagcc gtgagtcacc ggccacctgg accgggtcac gccaggcttc
    18421 gcgagcctgc gccaaccagc cgcggtgggg tgcctcgatg atctccaaca accgttcgat
    18481 ggaggtcaag gcaccgtcga cagtgcgaat atcggtcacc cacaccggga ttcccagctc
    18541 ccgcagggcg gtgacatccg cctcgcggtt ctcttccatg ctggcgacga ccaggtcggg
    18601 ggcgagcgtg gcgatccgcg gcacgtcggg gttcttggtg ccgcgcaccc gcacgacgtc
    18661 caaggacacc ggatgaacgc accagtcggt cgccccgacc aacaggcccg gcgcactcac
    18721 ggcgaaagcc tcggtcagcg acgggacgag gctcaccacc cgttcgatcg gtgaggtgag
    18781 gggcacggca tacccgaggt cgtcgacggt caccccgcca gtctgccggg ccacggggtc
    18841 gccgtgcggc ggagggcgca gtcgcccacg ccgagcccga ggtatgccgc ccgcggactg
    18901 tgcgcgcggc atacccctgc cctcgtcaga cgggcatcgg cgcgctggtg atgatgccga
    18961 acggctcgcc gtcggggccg gtgacgaccg ccaggcgtcc gtactcgaag tcgaagggct
    19021 cgaggatgac cccgccgccg ttctcgagga cccgcgcgac cgcggcgtcg gtgccgtcga
    19081 cctcgaaggt caccgaccaa tagggcgggc gctcggtctc gaggccgagg ccaccggcgg
    19141 gctgttcgcc gccgggcacc gagaacatcg cgtaggacat gccgtcggcc gacatgtccg
    19201 tgtaggtgaa gccgaaggcg gcagcgtaga acgccttgcc cgcctcgaaa tcacccacca
    19261 tgccctcaca ccagccgacc gcgcccggct cgttggcgac gtcgtagccg gtgtgcgacc
    19321 cggactccca ggtgccgaag accgcgccgg tcgaatcggc gtagacaccc atccggccga
    19381 ggtcggccaa ctgcatggag gggaagatcg tctgtccccc ggcggccgtg atcgctgcct
    19441 cggaggcggc cgagtcagcc gtggcgaggt agacggtcca gaacgtcggt gccgggtcgg
    19501 cacccggcat cgggggcgac atgccggcgg ccccccggcc gttgacgaga gccgttgtgt
    19561 aaccgccgaa ttcctcgctg cccggcgtga actcccaccc caggaccccc cgatagaacg
    19621 cctggctgcg cgccaggtcc ggaacagaga tgtcgaccca gcagggtgtc cccggcaccc
    19681 agctgccctc atgaatgctc atagctttcc ttccgcgagc tgatgatgag gtcaccctaa
    19741 ctctcggcgc cgacacgcct gtgctcagtc ggctgcgcac cccagcgcga cgcccaaccg
    19801 gtcgagagca gtggcgaagg gccgggccgg aagcaccggc tgaccggccg cctgcgcctg
    19861 ctcggccacc cactgcccgt aggtccacgc caggtgcgcc atcgcgtcgt gccaggggcg
    19921 tgggtgggcc ccggctcgtc gggtgtccat cgcccgccgg accgcgacgg ggtcggcctc
    19981 gtgcaggtat gccgcccgca ccggcaggtc agcagcgagc agtgccacac cggccggggt
    20041 gaaccacgac ccgtcgatga cgaggtgtcg gtgggctgcc gcctcgaagg cgagcaccgt
    20101 cggcaggacc tcctcaacca acccgccagc cgcgatctgc cgcgcgagca gcgcatcggg
    20161 gtcgactgga cccgcgcgca cggccccacg cacggccgag cggacgtcga gagcatctcg
    20221 ccgcgccgac cccggctcgg caccctcaag cagggccagc cagacggtgt ccagttgcaa
    20281 ccacccggct ccgagccgtg ccgccagcgc acgggcagcc gtggtcttgc cgaccccggc
    20341 tcctccgccg atggcgaaga cggtcaccga ccgagaccgg tcagaagttg atcatgtggc
    20401 cggcgatgcc gtgcagggcc tccttgacgg cctcacccaa cgtcgggtgg gcgaagacga
    20461 cccgcgacaa ctcgtcggcg gtgaggtccc acgtctgggc ggcgttgagc accggcagca
    20521 actcggtgac gtcggggccg atcatgtgcg caccgaggat ctcgttgtgc tcggcgtcgg
    20581 cgacgatctt gacgaagccg accgcgtcgc ccagcccctg ggccttgccg ttggcggcga
    20641 aggggaaggt ggccgtcttg acggcatacc ccttgtcggt ggcttgcttc tcggacaggc
    20701 ccatcgagcc gatctggggc tggcagtaag tggcccgcgg gatgaaggcg aagtcgatct
    20761 ccatcgtctc ggcgccggcg atggtctcgg cggcgacgat ccccatcgcc tcggcgacgt
    20821 gggcgagcat catcttggcg gtgacgtcgc cgatcgcgta gacgccgggg acgttggtgc
    20881 gcccgcgtcc gtcgatggcg atcgcgccgc ggtcggtgag ggcgaccccg gtggcctcca
    20941 agccgaagcc ggtcacccgc ggggcgaacc cgatggccga caggagccgg tcggcttcaa
    21001 ggacggtcga ctcgccaccg gcagcgggcg acacggtgac ccgaacgccg gacccggtgt
    21061 cctcgaccgc ctcgaccttg gtgccgagca tgaccttgac gccgagcttc ttgtagtgct
    21121 tggcgagttc cttggagacg tcctcgtcct cggtcgggac catccggtcg aggaactcga
    21181 cgatcgtcac atcgaccccg aaattgcgca tgacataagc gaattcgaca ccgatggcgc
    21241 ccgacccggc gatgatgatc gacccgggaa ggttggggtc gaggatctgc tcctcgtagg
    21301 tgacgacgtt ggcgctcacc tgcacgccgg ggatcatccg cgtgacggac cccgtggcga
    21361 tgatgaggtt gtcgaaggtg atcgagcgca ccgagccgtc ggccgccttg acgtccatcg
    21421 aggtcgggct ggtgagcgtc ccccagccgt cgatctcggt gatcttgttc ttcttcatga
    21481 ggaagtggac gcccttgacg atgccggccg acacctgccg cgagcgcgcg tgggtcggtc
    21541 cgtaggacat cgtcgcgtca ccctcgatgc cgtacttggc cttctcatgg gtgaggacgt
    21601 gcgccaactc ggcgttcttc aacagcgcct tgctcgggat acacccgacg ttgaggcaga
    21661 ccccacccca gtacttcagt tccaccactg cggccttcag accgagctgc gcggcgcgga
    21721 tcgccgcgac gtacccaccc ggaccggcac caaggacaac gacgtcgaag tcagccatgg
    21781 ggccagccta tggggtggcc tcgcggtgtt cgtcacgcag tgtctcgtag gtggcgacgc
    21841 ttcccgatac gtggacgtcg aagcccgccg gcactgggcc cagcaccttg gccggcaccc
    21901 cggcgaccat cgagccgggc gggatgacct gcccttcgcg gacgagcgcc gcggctgcga
    21961 cgagcgaccc gccgcccacc accgagtggt tgagcagggt ggcccccatc ccgatgaggc
    22021 tgccgtctcc caccgtcgcc ccgtggacga tcgccccgtg accgaccgtc acgtcacagc
    22081 cgagatcgac cggggcaccg gggtcggcgt ggaacaccgc gttgtcctgg acgttggagc
    22141 gcgccccgat ccggatcctc gcgcggtccg cgcggatgac ggcgccgtac cagatgctcg
    22201 ccccctcctc gatgacgacg tccccgatca ccatggcggt gggcgccacc caggcgcccg
    22261 ccgcgatcga gggcgtgtgg gggccgaaag gcaggatcat caggcaccat cccgtgggtc
    22321 gcaggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgc tacacgcctg
    22441 gcacccggac gccgacgtct tcatgctgca cggggagctc ctcgccgctc cccccgggga
    22501 gggtggccac tgaatccggc ccctgccgtc ccgcactcca ggccagggcc agcatcgcgg
    22561 tgccggccgc gacgaggagc agcaaggacg ccggtcccaa atgcactccc aaccacgggg
    22621 tcgcgacagc ggcgagcagt gccgccccga ccatcaccgt gtcacccagg ccgagcacgc
    22681 tggccctcaa ctcgtccggg acggtggcct gcaggacgct ggtcgccacg ttctcgcaac
    22741 acacggccac ggcgccgatg acggcgagag caccgaacga tgcagggccg cccggcagga
    22801 gagcgaacac cgcagccgcg ccggccaggg tgagcagggc ggcccgtccg gagcgcatcg
    22861 tccgcgagtg agcgacgacc ggtgccccga gagccccgaa gccgagcagg gcagtcgcca
    22921 gcccgaagcc ctcgtcgccc gatcgccaca cctcgtgggc gagttgcagc agcaacaacc
    22981 cgagcacgct gagcagcgcg ttgacaacgg caacgacggc gagcgcctgc cgagcgcagg
    23041 ggtcgcgccg caagagggac cacacgccgc cctcggccac gtcgatcgcg ctcgcctcag
    23101 gtgctggcat ggcggtgcgt ccgaaggcca cgatcgccac gccgacgaag atcgcaccga
    23161 tgagcgggac ccaggcgggc ggcatccgcc cgacgaacag gccgccgacg gcgggcccga
    23221 cgacgaagga ggccacctcg acggtgacca gcgcactggt ggcccgctcg gtcgcggccc
    23281 cggccaggcg cggcatacct gcagccaagg cggggtatgc cggtgtgccg gcgacgatcg
    23341 cacccgtcgc ggcgacgacg gcgaccagcg gatttgaggc agccagggcg gcggcagcga
    23401 ccagcagggc gaccgctcgc gcccagaggg agatccggac gatgagggct cgttcgcgcc
    23461 gatcggccag gcgcccggac agccaactga gggccacata cggcgccagc ctcgcggctc
    23521 cggccaggcc aagcagcaac tgggacgacg tggactgctc gacggcaagc aacagcaccg
    23581 gccagggcag ccccatgccg accgctgcga aggcatgcga ggtgatgacc cgacgcacct
    23641 cgggacgacg cagcgcgggc tcgacggcca tctgggctcc tgcgggagtc ggcgactttc
    23701 ccacaaagac cctggacccg ggccgctgat accccgcagc gcaggcggat tgcgcctgac
    23761 ccagccccgg gtcagcccac gtgccggttg tgcccggctc gcaccagcgc ccacggcacc
    23821 aggaccgcaa gcgcagcgat cgggtaggcc catccgccga gcgcggtgtc ggcggcatac
    23881 gacccgccga gccaggcaca cgcgacggag gccgcgagga accacagttg tgacagcgga
    23941 gccaccgtcg gtgaacggaa ccacgacagc cgctcccccg ggttgttgcg ggcgagcacc
    24001 gtcaccgaga tccacgagca ggccgccgct gcgagcacga acaaggtcca ccacggcaac
    24061 gccatgctcg cctcagatct cgtgcagggt gctggccagc cgagcgatcg cgctgtcgac
    24121 atcggcgcgt ttgcgcgcgg ccacctcgaa gacgatgcag tgccgaccca catcgacgat
    24181 gaaggtcgtc aacgcgatcg aggccccctg gaccgtgacc gtgccattga tccgcagcgc
    24241 gtcgcccacc cgggtcgcca gtcgttcgct ccccgacacc ttgaccttgc cctcggagta
    24301 ggtcgtcttc acccagtcgt catcgggcaa cgaaccgacg tcggcaacgg tcgccgacag
    24361 gttggtggcg taacgattga ccggggccgc gacgtaagcg atctcgatgt cgtcgaaccg
    24421 gtcgatcagc tcgcgggccg agatgcccag cgccttggcg atcgccttca cgtccgccgg
    24481 gatgctcgca gtggtcgtca tggtggcggg cacgatctcg acccaggccg acgccacggc
    24541 gaagcgcacc agctcgctgt cgacggtcgc ccacttggct cccgcaggaa gggccggtgg
    24601 cgggggcgac gcgctcggct tcggggaggg gctgctcgac gtggacggcg tgggggacgg
    24661 cgagggcgac ggcgtcgcgc cggtcgcctt gggcacggcg ccggtggcga acgggtcaaa
    24721 cgtggcgatc gacgtgggcg ctgcggtggc cggcccgttg ctggcgttga gcgcactcac
    24781 cacgaggcac cccgacaaca gggggaccaa ccccccgatc aacaccatcc ccctgagacg
    24841 cgtcatgcca gacatcgtag gttgc
//
LOCUS       DAORPW010000017        24181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_333_length_24181_cov_727.738456, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000017 DAORPW010000000
VERSION     DAORPW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 24181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24181
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_333_length_24181_cov_727.738456"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..967
                     /locus_tag="PLB86_02845"
     CDS             158..967
                     /locus_tag="PLB86_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC64101.1"
                     /translation="MTDDRAAQTAPVLLHVEGGVATVTLNRPEAMNSLDLATKDLLVP
                     TIRQVADDPAVRVVVITGSGRAFCVGQDLKEHIAGLMAGDEGLGVTVVKHYNPIAEML
                     ATMNKPVIAALNGVAAGAGASIAMAADFRIMVEGGGMNLAFAGIALSCDTGSSFWLPR
                     LVGVAKAKELLLLPRTLSAQECLDLGLVTSVVPADAFAGEVATLAGKLAAGPTLAYGA
                     MRQAIAFSAAHSLTESLAFESDLMNSTGQTADHRVAVDAFLAKQKPVFEGR"
     gene            complement(980..2143)
                     /locus_tag="PLB86_02850"
     CDS             complement(980..2143)
                     /locus_tag="PLB86_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64102.1"
                     /translation="MVQVVVLYAITRLLTTVMLALVAPSQLPADMTDHQAVDYVGFTR
                     LWDGQWYERVATGGYPNTVPRDPAGIAMQNTWAFYPLFPFLARAVMEVTGLSFAAAAS
                     TLALVIGFGAAVAMAALLRPRIGRAGALLVVGLYAVFPSSPALQVAYTESLAMLLLCG
                     YLLALSRERWLVAAGLALLIGVTRPIALPLGVVTLVAVWLRWRRRAAEPMSPGETGAA
                     LTSLVGCGVAGLLWPGIAWVATGQASAYFDTMGAWSMTGHVQILEPWFTIPRYYLGDW
                     GPRLFAVTLVLLLVGMAGPWARRLGPELRVWPVIYAAYVIAVQTPGTSTARYLLPMFP
                     YLAVLLGLASERGRARGIPTTVRALVLGAAFLWLQWKWVSVVWLFTPPIDFAP"
     assembly_gap    2165..2264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2265..2472)
                     /locus_tag="PLB86_02855"
     CDS             complement(<2265..2472)
                     /locus_tag="PLB86_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64103.1"
                     /translation="MGVARGRRSVGRPLDASPASVAAAAAAADSAPDSKAALSGPPFA
                     RAEAVLDRVFRGNAVVQVVVLYAIT"
     gene            complement(2483..3097)
                     /locus_tag="PLB86_02860"
     CDS             complement(2483..3097)
                     /locus_tag="PLB86_02860"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRC64104.1"
                     /translation="MSATAVVTGPDGIPRCAWAASTADYVAYHDDEWGVPLHGERELF
                     ERLTLEAFQSGLSWLTILRKREGFRAAFAGFDPDVVAAFDADDRARLLADTGIVRNVR
                     KVDAAIHNAQAVVDLRDDGGLDALVWAHAPAHHDRPQGMGDVRATSPESVALTKALKA
                     RGFVFLGPTTMYAAMQACGLVDDHLAGCHRAGGQQARPAAPEPR"
     gene            complement(3094..3528)
                     /locus_tag="PLB86_02865"
     CDS             complement(3094..3528)
                     /locus_tag="PLB86_02865"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC64105.1"
                     /translation="MTAVTIQRVVPRPIEAVWAVVSDLESHPVTLTRIDTDPGPPRVG
                     WAFVALTGIGRLRFADRMVVTRWSPPADGRAEFAIVKTGRWLGGWATIRLEALDGTRT
                     RLTWREEIVPHPHAIGRLAAPLTDRAVRSIFTRAVAEMAARA"
     gene            complement(3532..3918)
                     /locus_tag="PLB86_02870"
     CDS             complement(3532..3918)
                     /locus_tag="PLB86_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRC64106.1"
                     /translation="MWIALVVAVVLVGLTVAAVFGRVDGSLVDPTSSLSYAPLPDGPL
                     SPDDVQGLRLDTGLRGYRMEQVDEVIDRLTAEITVLRDQIDAATTAAYQPGPDADPGM
                     PPASPFVRPSEWAEFSEPIARPDSQA"
     gene            complement(3918..4673)
                     /locus_tag="PLB86_02875"
     CDS             complement(3918..4673)
                     /locus_tag="PLB86_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRC64107.1"
                     /translation="MNGKDRRRSYQKGPVTLRGQRVPETTTDQRLLDSRGSTEWLHAD
                     PWRVMRIQSEFVEGFGALAELGPAVSVFGSARLGAGHPAYELGVQVGRRLVESGYAVI
                     TGGGPGLMEAANKGALEAGGTSVGLGIELPFETGLNDYVDLGVNFRYFFARKTMFVKY
                     AQGFIVLPGGMGTLDELFEALTLVQTGKITTFPIVLLGCDYWAGLIDWFRTTLVAAGT
                     ISPDDVERILVTDDVEQAVRFVVDQDHLHTEVR"
     gene            complement(4929..6011)
                     /gene="dapE"
                     /locus_tag="PLB86_02880"
     CDS             complement(4929..6011)
                     /gene="dapE"
                     /locus_tag="PLB86_02880"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HRC64108.1"
                     /translation="MPLTSPSLDLAADVVTLTAAICDIESVSGEEGPLADAIEAALDG
                     LAHLEVVRDGHTVIARTALGRPERVVVAGHIDTVPLTRHTPNLPTRRVGDELVGRGTV
                     DMKGGVAVMLKLAAALTAPNRDVTYCFYDCEEVDSERNGLRRVAEHRPELLAADFAVL
                     MEPTDGTVEGGCKGTLRVEVRTKGIASHSARPWVGHNAIHDAGEVLARLVAYEARVVD
                     VEGLTYREALQAVLISGGTASNVIPDACAVTVNYRYAPDRSEDEAVAHVREVFAGFEV
                     EVTDNAGGARPGLDRPAARAFVEALGVPVQAKEGWTDVARFAALGVPAVNFGPGNPLL
                     AHMDDERAPVQQYVDAEAAMFRWLQS"
     gene            6110..7039
                     /gene="dapD"
                     /locus_tag="PLB86_02885"
     CDS             6110..7039
                     /gene="dapD"
                     /locus_tag="PLB86_02885"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRC64109.1"
                     /translation="MSERTAWGFGLATVTKHGDVLDAWFPQPSLGTPPDDVAAPTAIA
                     ELAGKDKVRKVTQAVVLVIADLDSPPADTADAYLRLHLLSHRLVAPNSINLDGIFAAL
                     PNVVWTNHGPCAVDGFEQTRARLRAQGPVTVFGVDKFPRMVDYVVPSGVRIADADRVR
                     LGAHLASGTTVMHEGFVNFNAGTLGASMVEGRISQGVVVGDGSDIGGGASTMGTLSGG
                     GTERVRIGRRCLLGAESGLGIALGDDCVVEAGLYVTAGTKVTLPDGQVVKARELSGQD
                     NLLFLRDSVSGTVVVKSRDGHGIALNAALHAND"
     gene            7054..7932
                     /locus_tag="PLB86_02890"
     CDS             7054..7932
                     /locus_tag="PLB86_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64110.1"
                     /translation="MPRTPAGLILAEQGPPSPRRRRVVRVFVAALVLVVAAAGWVGVR
                     TILASLSPEACWTTSPSARVEVTPEQAGNAATISAVAVSRGLSSRAATIAIATALQES
                     KLRNLRYGDRDSVGLFQQRPSQGWGTVEQILDPVYASNAFYNKLVKVKDYLTRPLTEV
                     AQEVQRSGAPAAYAQHEDEAQALAAALTGAAPATLVCRLDPPSAVPAAKDLRAAITNE
                     FGTPTAGSGGTVTVAAEGATAWALASWAVAHADARGVSRVEVGGRTWSRSDPQTWQST
                     PSGPQDTVTITLVTAL"
     gene            complement(7973..9274)
                     /locus_tag="PLB86_02895"
     CDS             complement(7973..9274)
                     /locus_tag="PLB86_02895"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRC64111.1"
                     /translation="MTDTETATHASLHVGARDASLQIVPSAQGEPGLNVGALLKDTGF
                     VTYDAGFVNTAACRSAITFIDGDVGILRYRGYPIEQLAEKSSYVEAAFLLIYGELPTP
                     SQLADFDSRIRHHTMLHEDLRNFFSGFPRDAHPMPVLSSAVSALSTFYQDSLDPFDPM
                     QVEISTIRLLAKLPTIAAYAYKKSIGQPFLYPDNNLSLPENFLRMTFGTPAEPYEVDP
                     VHAKALDLLLLLHADHEQNCSTSTVRLVGSSHANLFASVSAGINALFGPLHGGANQAV
                     LEMLNSLRESGESPEAFMRRVKNKEGGVRLMGFGHRVYKNYDPRASLIKKAADDVLAK
                     LGVDDPLLGLAQELEAIALADDYFVERRLYPNVDFYTGLIYKAMGFPTKMFTVLFALG
                     RLPGWIAQWREMMTDPETKIGRPRQIYTGATERDYVGIDYR"
     gene            complement(9340..10497)
                     /gene="dapC"
                     /locus_tag="PLB86_02900"
     CDS             complement(9340..10497)
                     /gene="dapC"
                     /locus_tag="PLB86_02900"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HRC64112.1"
                     /translation="MPLTPPSLPDFPWDSLVPFRRIAAAHRDGIVDLSVGTPVDPTPQ
                     AIQAALVAAADSPGYPTTVGTPALREAVVAWFERRRGVGGLSPDAVLPTIGSKELVAW
                     LPTLLGVGAGDLVGIPAVAYPTYDVGARLAGAAPIVVDGLAALGPLTPSTTPKVLWLN
                     SPGNPTGAVLPVEHLAKVVAWARRHGVVVAADECYAELDWRQPDPRRPGPRHTTPSLL
                     DPAVCGGSHEGLLAVYSLSKQSNLAGYRAAFVAGDPALIAGLVEVRRHAGMMVPGPVQ
                     AAMTVALRDDEHVADQRLRYGVRREILIEALEGVGLRIEHSEAGLYLWATRDEDCWDT
                     VGWFAERGILVGSGSFYGEAGRRHVRVALTATDERIAAAAGRLSVGSHREG"
     gene            complement(10507..10833)
                     /locus_tag="PLB86_02905"
     CDS             complement(10507..10833)
                     /locus_tag="PLB86_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRC64113.1"
                     /translation="MTYVIALPCVDLKDKACIEECPVDCIYEGERMLYIHPDECVDCG
                     ACEPVCPVEAIYYEDDVPAEWSDYYKANVEFFDDLGSPGGAAKLGLIPKDHALVSALP
                     PQASEH"
     gene            10966..11715
                     /locus_tag="PLB86_02910"
     CDS             10966..11715
                     /locus_tag="PLB86_02910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC64114.1"
                     /translation="MTDGWRALEEVWLGDWLLRASHGFTQRGNSALVIDDPGLPLAAA
                     VSTVERWYAARDLPPRFCLVTDVAGSAADPSVSTRLAQSGYVSRSATYTMTAFTADLP
                     GPPTGARDVVVDDQVTPAWLAAFAAYRRLVPGVAEAILAGSHGQLFLSVPGEGSADAP
                     PIAIARMSTYPQWAGIHAMWVAPEHRREGVATVVVAAVARLAAERGLPGVYLQVERSN
                     SVATAAYDGWGFRAHHGHVYYAPADDGLGHS"
     assembly_gap    11768..11867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11881..13548)
                     /locus_tag="PLB86_02915"
     CDS             complement(11881..13548)
                     /locus_tag="PLB86_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRC64115.1"
                     /translation="MREWSRIVVRSVVAVAILAGAYVAVAWWTGRQVPAALVVEGVSV
                     GGMSTEQAAERLTTELAAKAKTPIRVDLAGTQSSVSIDPVASGLSFDIPGTLDGLTGF
                     TLDPTVIWARLSGRTDRPILVKADEPRLLAALTEKAATVQTTAVDGTVSFAGGKVATT
                     PAVDGVALQLDDAVRKVVQAWPRVESVTAAASITAPKVTKAKVDAAVTSFAAPAMSAP
                     VTIVVGDKTAVVTPEQYAEALSMVPDESGTLNPVVDKEALRGVVASATSAVVVAPKDA
                     TIVLEGDKPVIRPSVDGVSVDTASAADLLVKALTAPDRRVSLSTVAAKATLTTEGAQA
                     LGVNEVIASFDSAYPYDPPRTNNMTLGAAAVNGTLIKPGEVFSLNKVMGERTSAKGYQ
                     EAGVISNNRLTKNVGGGVSQISTVTYNLAWFAGVELTAHKAHSFYISRYPAGREATVS
                     WPDLDNKWTNNSPYGILVQMWLSGGQVHGRMWSTKVYDVTAVAGPRTNIKHGRSITDS
                     SPSCVPHAFTDGFDITVQRVFSKGGSVVKRESYSTTYVPADQIICSG"
     gene            complement(13633..>14121)
                     /locus_tag="PLB86_02920"
     CDS             complement(13633..>14121)
                     /locus_tag="PLB86_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRC64116.1"
                     /translation="DPVDPGRFRTAFGRFATGVSVVTCLVDGSDHAMTANSLTSVSLA
                     PPLVLVCVERETRFHEAILTAGHWGVSVLGAQARGAAAWFATRGRPLAGQLDPVAHHR
                     SSLTGVALLDDALAWFEVRTTAVYDGGDHSIVVGEVVALSLAEEPPAALTFFRGQYGH
                     LD"
     assembly_gap    14122..14221
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14225..15097)
                     /gene="mshB"
                     /locus_tag="PLB86_02925"
     CDS             complement(14225..15097)
                     /gene="mshB"
                     /locus_tag="PLB86_02925"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HRC64117.1"
                     /translation="MARLLFVHAHPDDETLATGIAIAHYVSRGHDVEVLTATLGEEGE
                     VIPADVRHRTSDRDDTLGEFRAGELAGAMARLGARHTFLGDDGSGPRWRDSGMAGMPS
                     ADHPRAFAGADVAEAARLVAAYLRERRPDVVVTYDVDGGYGHPDHIQTRRVVQAAITG
                     LAPAERPGRFFETLTPRSWVVADRQWLAEQVPEASGLHIPTQNEPYAVSVVDDAVVSH
                     VVVDEMAGKRMTWALAAHRTQVTLFDGYYSLSNDIAARLSGREAFARLDPATGERLPR
                     TSDTWFEGLLVDLP"
     gene            complement(15097..16134)
                     /locus_tag="PLB86_02930"
     CDS             complement(15097..16134)
                     /locus_tag="PLB86_02930"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRC64118.1"
                     /translation="MTIQTSTGAAPSATPAGRPLLRLTNLTKHFPQFRQTLIKRPDNP
                     VRAVDGISFDVAPGQTVGLVGESGCGKSTAGRTLLRLLEPTAGTIEFEGRDITHAKGA
                     ELRTLRREMQMIFQDPYGSLNPRQTIGSIIAAPYDIQKITPEGGRRAAVQGLMERVGL
                     NPEHYNRYPHEFSGGQRQRIGVARAIALKPKLIVCDEPVSALDVSIQAQVVNLLEDIQ
                     EEQGLAYVFIAHDLSVVRHISDRVIVMYLGKIMEEADRDELYEAPLHPYTHALLSAVP
                     VPDPKRIEHKERILLKGDLPSPQNPPSGCVFRTRCPKAQERCTVEVPVPVELSPGHRV
                     ACHFPEARTVL"
     gene            complement(16145..17230)
                     /locus_tag="PLB86_02935"
     CDS             complement(16145..17230)
                     /locus_tag="PLB86_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC64119.1"
                     /translation="MADTSGATVTTSPSARAAGERTAYLDVRDLKVHFPTDDGLVKAV
                     DGLNFTVERGKTIGIVGESGSGKSVTSQGILGLHRRTRARASGQIWLDGEDLMTVTDE
                     RLRELRGNKMAMIFQDPLSALHPYYTIGSQIVEAIRVHHSDTSKSAAKAKAVDLLGLV
                     GIPNPARRVDQYPHEFSGGMRQRAMIAMALANDPSLLIADEPTTALDVTVQAQILDLM
                     RKLQAEFGSAIIMITHDLGVVAEMADDILVMYGGKAVEHGPVNDVFYEPEHPYTWGLL
                     TSMPRLDRARLSRLNPVPGNPPSLINVPSGCAFHPRCAFTGQVPADACRAAVPDLTAA
                     GPGHLTRCHLEPATRAELFASEIKPRL"
     gene            complement(17233..18246)
                     /locus_tag="PLB86_02940"
     CDS             complement(17233..18246)
                     /locus_tag="PLB86_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC64120.1"
                     /translation="MFFYIIRRLISVVVMLFIISMTTFLVFFASPVDPASLTCAKNCT
                     PAVIEGNRKLLGFDKPITVQYANFVKGLFVARDFPDDPEQKARAPQTVFTCAAPCLGY
                     SQRQAKSVTEIIKDGLPITASIALGAFVLWITSGVFFGIIAALNRGRWIDRLLVGVSL
                     IGYSLPSFFIGQLLLVFIAIKWQLWPIPNFVPLSEGLWPWMQSIFLPSLTLAAIFGAS
                     YVRLTRAYMLETMSEDYIRTARAKGVKESVVVRRHGLRAALTPIVTAAGLDLGGLLGG
                     AIISEQVFSFRGLGWTSIQAITNTDLPVIVAVVVVASFFIVIANLVVDVLYGVIDPRV
                     RMS"
     gene            complement(18385..20112)
                     /locus_tag="PLB86_02945"
     CDS             complement(18385..20112)
                     /locus_tag="PLB86_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC64121.1"
                     /translation="MMRMKKGAAAVALGAAVAMTAAACSSGSTPSTTTASTAAAVAKG
                     GTLYILNLGPHNGLDVQQSYVGADLQTANRVYMRYLTSYSTGANPKLVPDLATDLGTM
                     SDGGKTWKFTIVDNAKWQDGAKVTCEDVKYGGSRTFATDLISNGPTYSLQFLDIPNDA
                     DGTSSYKGPYNGTGQALYDKAVTCDGQTLTLHFKTPWADFNQATAALGIFAPFPKAQD
                     KADKSLFAPWSNGPYMLEGTFDPAKGGTWVRNPNWDAATDPIRKAYPDKIVDVEGIQG
                     ETVYQRLLADGGDDKTAITFGTAPPSVLPTIASTPGADKRMANVPAPYVDYIQPNMTS
                     TAMSNPKVRQAFAMATNRDAYITANGGPQVMVPTYAMCNKVLACYKDFNPFGAPTAGD
                     PDGAKKVLTDAGVAMPVNITVVYRKTPTRDKSFAALKETWDKAGFNVTLEGITDKYYR
                     TISGAAMKTKDAFYASWGADWPSGSTDIPPLFDGRINITPTSSNQDYGYYNSDAANKA
                     MDAAYLITDPAAREKAWGDIDEMISKDGGVIPLVNQKFTFVYGSGVKNFEVNTLFGGY
                     ADLANIAVK"
     gene            complement(20266..21327)
                     /locus_tag="PLB86_02950"
     CDS             complement(20266..21327)
                     /locus_tag="PLB86_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC64122.1"
                     /translation="MTVAPGEPENRSDASAVPVGSAVAVDPGSPVALDGVDGAVAIAG
                     RTPWQIVMRRLRRDRVTMVAFGASVFFVALAAIAPLLARIGILNPDQTHPELVQGLGS
                     MPEGLMGGVSWSHWLGVEPGTGRDLLSRIVTGMTTSLTVALLATLFSVVVGTIIGIIS
                     GFAGGRVDWLLSRSMDLVLSFPSTLMLLSLQAVLVDRIASIMGQPSSAPPPKIAFMVL
                     VLGFFGFPFFARIIRGQVLSLREREFVEAARSLGARRGRLYFKELLPHLWAPLLVYTT
                     LVLPANISAEAALGFLGVGLNPPTASLGAILNDTVSYALPDPAYFFFPGVTVFLLVLS
                     FNLLGDGLRDALDPKAGRQ"
     gene            complement(21466..>22737)
                     /locus_tag="PLB86_02955"
     CDS             complement(21466..>22737)
                     /locus_tag="PLB86_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC64123.1"
                     /translation="ESGSGSAEFDAAVSCRGATLTMRLKVPVPDFPVIVALPTFAAYR
                     RDHDRGGAGTFDVFSCGPYRLDGAWEAGAGGRFVRNPSWNRAGDALRQALPDVVDIRE
                     SIPVNTLVQRLVEDKAPDHTAIGITDIPAAGHAALLADPAVKARLSNPLSGTVELLQP
                     NVKSPVMSNEGVRRALALATDRAAFVAAYGSTVMTPAYSALASSIPGHPATPPAADAT
                     PTATASATANSTAPLPTAADPDAARAALVAAGVTLPVHIRVAYRTSPAADAAYAALKA
                     GWEKVGFEVTLDGLGEDYYRIVSGIDASANYDVFRRSWFADYPAGAAVIPDLFDGRMN
                     LTDSGTGQDLGSFNDEAVNTAIADAQVEADPVKRALAWAAIDAKIVAVVGQIPLAERR
                     RSFLRGSGVGGYAENPYLGGWVDLAGVSVAH"
     assembly_gap    22738..22837
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22838..23366)
                     /locus_tag="PLB86_02960"
     CDS             complement(<22838..23366)
                     /locus_tag="PLB86_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC64124.1"
                     /translation="MRGSGSPRYAARAAYLFLASVLLTGACTGSPNSPSPTEAAGGTL
                     RLLYDQTITTWDPQRMYSGPEGAIAVRLFSRTLTSYPAGGAGGVGGLVGDLATDTGTP
                     SDGGKTWTFTLRSGPAWEDGKPVTCQDVAYGIARSFAREQLPGGAPYPMTLLDIPSRV
                     DSAGLEVPGYPGPYAV"
     gene            complement(23372..>24181)
                     /locus_tag="PLB86_02965"
     CDS             complement(23372..>24181)
                     /locus_tag="PLB86_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRC64125.1"
                     /translation="VSIRVLNTDRPDTWEVQGRGELALAILVEQMRREGFELTVGKPQ
                     VVTKQVDGKVHEPMERLTIDTPEEFLGVVTQLMAARKGQMEHMTNHGTGWVRMEFLVP
                     SRGLIGFRTEFLTETRGTGIAHHVFEGYAPWAGPITTRINGSLVADRSGAVTAYAMVN
                     LQERGTLFVEPTTEVYEGMIVGENSRADDMDVNITKEKKLTNIRASSSDNFEKIVPAR
                     KLSLEQSLEFCREDECVEVTPDAVRIRKVVLDQVERGRTASRARSAAKAEN"
BASE COUNT         3785 a         7942 c         8432 g         3622 t
ORIGIN      
        1 cggtcctcgg cttcatcgcc gccatgggtt ccccttcctc ggaaactcgt gcgccacagc
       61 ctgatgcgtc ggcagattca cgacgcagac tgcaccggag accattatca cgcgtctacc
      121 gggctcggac gaaatcgtcg tgcggcacga tggggccatg accgacgatc gtgccgcgca
      181 gaccgcgccc gtcctgctcc atgtcgaggg cggggtcgcc accgtgaccc tcaaccgtcc
      241 cgaggcgatg aactccctcg acctcgcgac gaaggacctc ttggtgccga cgatccgcca
      301 ggttgcggac gatccggcgg tgcgcgtcgt cgtcattacc ggaagcggtc gggcgttctg
      361 cgtcgggcag gacctcaagg agcacattgc cgggctgatg gccggcgacg aggggctcgg
      421 ggtcaccgtc gtcaagcact acaacccgat cgccgagatg ctcgccacga tgaacaagcc
      481 cgtcatcgcc gccctcaacg gcgtcgcggc cggcgcgggt gcctcgatcg ccatggccgc
      541 cgatttccgg atcatggttg aaggcggggg catgaacctc gcgttcgccg ggatcgcgct
      601 ctcctgcgac acgggttcgt cgttctggct gccgcgcctc gtcggtgtcg ccaaggccaa
      661 ggagctgctc ctgctcccgc ggaccctctc ggcgcaggag tgcctggacc tcggcctggt
      721 caccagcgtc gtcccggccg acgccttcgc cggcgaggtc gccacgctgg ccggcaaact
      781 cgccgccggg ccgaccctgg cctacggcgc gatgcgtcag gcgatcgcct tcagcgcggc
      841 ccactccctc accgagtcgt tggcgttcga gagcgatctg atgaactcca cgggccagac
      901 cgccgaccac cgggtcgccg tcgatgcctt cctggccaag cagaagccag tgttcgaggg
      961 tcggtaaccg gtcgggccgt tagggcgcga agtcgatcgg tggcgtgaac agccacacga
     1021 ccgagaccca cttccactgc aaccagagga aggccgctcc gaggacgagc gcccggaccg
     1081 ttgtcggtat gccgcgcgcc cgaccccgct cgctggccag ccccagcagg accgcgaggt
     1141 aggggaacat cggcagcagg tagcgcgccg tgctcgtccc aggggtctgc accgcgatga
     1201 cgtatgccgc gtagatcacc ggccagaccc gcagctccgg ccccagtcga cgagcccacg
     1261 ggccggccat gccgacgagc aggagcacga gggtcacggc gaacagccgc ggcccccagt
     1321 cgccgaggta gtaccgcggg atcgtgaacc acggctcgag gatctggacg tgcccggtca
     1381 tcgaccaggc gcccatggtg tcgaagtacg ccgaggcctg gccggtggcc acccaggcga
     1441 tccctggcca caacaggccg gccactccgc acccgacgag gctggtcagc gccgctccgg
     1501 tctctccggg gctcatgggc tctgcggctc gccgccgcca gcgcagccac accgcgacaa
     1561 gcgtgacgac ccccaagggc agggcgatcg gccgggtgac cccgatcaac agagccaatc
     1621 ccgctgcaac gagccagcgt tcccggctca gggcgaggag gtatccgcac aacagcagca
     1681 tggccaacga ctcggtgtag gccacctgca acgccggaga ggaggggaag acggcataca
     1741 ggccgacgac gagcagcgcg cccgcgcgac cgatccgggg gcgtaggagg gccgccatgg
     1801 ccaccgccgc gccgaacccg atgaccaagg ccagggtcga ggcggcggcc gcgaacgaga
     1861 ggccagtcac ctccatcacg gcgcgggcga ggaacgggaa cagcgggtag aaggcccagg
     1921 tgttctgcat ggcgatcccg gcagggtccc ggggcaccgt gttgggatat ccgcccgtcg
     1981 ccacccgctc gtaccactgg ccgtcccaga gtcgagtgaa accgacatag tcgacggcct
     2041 ggtggtcggt catgtcggcc ggcagttggg acggcgcgac gagggccagc atgacggtcg
     2101 tgagcaggcg cgtgatcgcg tagaggacga cgacctgaac cacggcgttg ccgcggaaga
     2161 cccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgtga tcgcgtagag
     2281 gacgacgacc tgaaccacgg cgttgccgcg gaagacccga tcgagcaccg cctcggcgcg
     2341 agcgaagggt ggcccgctca acgcggcttt ggagtcgggt gcagagtcgg ctgcggctgc
     2401 ggctgcggct acggatgcgg ggctggcgtc gagcggccgt ccgacactcc tccgcccgcg
     2461 tgcgaccccc attcgggtca tgctaacggg gctcgggggc agccgggcga gcctgttggc
     2521 cgccagcccg atggcacccg gccaggtggt catcgacgag cccgcaggcc tgcatcgcgg
     2581 catacatggt cgtcgggccg aggaagacga aaccacgcgc cttgagcgct ttggtcagcg
     2641 cgaccgactc cggcgaggtg gcccgcacat cacccatccc ctgcgggcga tcatggtggg
     2701 cgggggcgtg ggcccagacg agcgcatcga ggccaccgtc atcgcgcagg tccacgaccg
     2761 cctgggcatt gtggatcgcc gcgtccacct tgcggacgtt gcggacgatc ccggtgtcgg
     2821 cgagcagccg ggctcgatcg tcggcgtcga aggcggccac cacatcgggg tcaaagccag
     2881 cgaaggcggc ccggaagccc tcgcgcttgc gcaggatggt gagccacgac aacccggact
     2941 ggaacgcctc gagggtgagc cgctcgaaca actcacgctc gccgtgcagc ggaacgcccc
     3001 actcgtcgtc gtggtaggcg acgtagtcgg cggtggacgc agcccacgca cagcggggta
     3061 tgccgtccgg ccccgtgacg acggcggtcg cactcatgcg cgggcggcca tctcggccac
     3121 tgcgcgggtg aaaatcgagc ggacggcccg atcggtgaga ggtgcggcca gccgaccgat
     3181 ggcatgcggg tgcggcacga tctcctcgcg ccaggtcagt cgcgtgcggg tgccgtcgag
     3241 cgcctcaagc cgaatggtgg cccaaccccc cagccaacgg ccggtcttga cgatcgcgaa
     3301 ctcggcccgc ccatcggccg gcggcgacca acgggtgacg accatccggt cggcgaagcg
     3361 cagccggccg atgccggtca gcgcgacgaa cgcccacccc acccggggtg gtcccgggtc
     3421 ggtgtcgatc cgcgtcaacg tcaccggatg cgattccagg tcgctgacga ccgcccagac
     3481 ggcttcgatc ggccgcggga cgacccgctg gatggtgact gcggtcatgg atcaggcctg
     3541 cgagtcgggg cgggcgatcg gttcggagaa ctccgcccat tcggaggggc gcacgaacgg
     3601 cgaggcgggc ggcatacccg ggtcggcgtc ggggccaggc tggtaggcgg ccgtggtcgc
     3661 ggcgtcgatc tgatcgcgca ggacggtgat ctcggcggtg agccggtcga tgacctcgtc
     3721 gacctgctcc atccggtagc cccgaaggcc cgtgtccagc cgcaggccct ggacgtcgtc
     3781 aggagacagc ggaccgtccg ggagcggggc gtaggacaac gacgacgtcg gatcgacgag
     3841 cgacccgtcg acccggccga agactgcagc gaccgtcaac ccgacgagga cgacggctac
     3901 gaccagcgca atccacatca gcgcacctcc gtgtggaggt gatcctgatc gacgacgaag
     3961 cgcacggcct gctcgacgtc gtcggtgacg aggatgcgct cgacgtcgtc tggcgagatg
     4021 gtgcccgcgg cgacgagggt cgtgcggaac cagtcgatga gccccgccca gtagtcgcac
     4081 cccaagagga cgatggggaa ggtcgtgatc ttgccggtct gcacgagggt cagcgcctcg
     4141 aacagctcat cgagggttcc catgccgccg ggcaggacga tgaagccttg ggcgtatttg
     4201 acgaacatcg tcttgcgggc gaagaagtag cggaagttga cgccgaggtc gacgtaatcg
     4261 ttgaggccgg tctcgaacgg caactcgatg cccagcccga ccgaggtgcc gccggcttcg
     4321 agggcgccct tgttggccgc ttccatgagg ccagggccgc cgccggtgat gacggcatac
     4381 ccggattcga ccagtctgcg gcccacctgg accccgagct cgtatgccgg gtggccggct
     4441 cccagccgcg ccgacccgaa gacgctgacg gccgggccaa gctcggcgag cgccccgaac
     4501 ccttcgacga actcggactg gatgcgcatg acccgccacg ggtcggcgtg gagccactcg
     4561 gtggagccgc ggctgtccag caggcgttgg tcggtggtcg tctcgggcac gcgttggccg
     4621 cgcagggtca ccggaccctt ctggtacgac cgtcgccggt ccttgccgtt cacgagaggc
     4681 aacgctacgc gaccgggctg ggtgctcagc gcagcgacgg ccatccgggg gctgccgatc
     4741 aggcgaaccc gggtttgcgt catgccaaac gcgcggcata cgtcatcggt tggtcggcgg
     4801 gcgggaggcc gcgcatgaag ccgcgaccgg tggcgtgatc acgcatgcag cgacggcttc
     4861 acgggcggct tcatccccgc ggctggcggg cggacggcat gacgctcatg atgctcggcc
     4921 ggggtgggtc aggactgaag ccaccggaac atcgcggcct cggcgtcgac gtattgctgg
     4981 accggtgccc gctcgtcgtc catgtgcgcc agcagcgggt tgccgggccc gaagttgacg
     5041 gccggcacac ccagcgcagc gaaccgcgcc acgtcggtcc agccctcctt ggcctggacg
     5101 gggaccccca acgcctcgac gaaggcacgc gcggcgggcc gatcgagccc cggacgcgcc
     5161 ccgccagcgt tgtcggtcac ctcgacctcg aacccggcga agacctcacg cacgtgagcg
     5221 acggcctcgt cctcggagcg gtcgggcgcg tagcggtagt tcaccgtcac ggcgcacgcg
     5281 tcggggatga cgttggacgc cgtccctccc gagatgagca cggcctggag cgcctcgcga
     5341 taggtcagcc cctcgacgtc gacaacccgc gcctcatacg ccaccagccg cgcgagcacc
     5401 tcgccagcat cgtggatcgc gttgtgtccc acccacggcc gggccgagtg cgaggcgatc
     5461 cccttggtgc gcacctcgac ccgcaacgtc cccttgcacc caccctccac ggtgccgtcg
     5521 gtcggctcca tgagcacggc gaagtcggcg gcgagcaact cgggtcggtg ctcggcgacc
     5581 cggcgcaggc cgttgcgctc cgagtcgacc tcctcgcagt cgtagaagca gtaggtgacg
     5641 tcgcggttgg gcgcggtcag ggctgccgcc aacttgagca tgacggccac cccgcccttc
     5701 atgtcgacgg tcccacgccc gaccaactcg tcgcccacgc gccgggtcgg caggttgggc
     5761 gtatgccgcg tgagcggcac cgtgtcaatg tgcccggcga cgacgacccg ctccgggcgc
     5821 cccagcgccg tccgggcgat gacggtgtga ccgtcgcgga cgacctcaag gtgggccagc
     5881 ccgtccaggg ccgcctcgat ggcatcggcc aacgggccct cctcgccact gaccgattcg
     5941 atgtcgcaga tggcagcggt gagggtgacg acgtcggcgg caaggtccag cgaaggcgag
     6001 gtgagcggca tacccctcag gctaggggta tgccgctcgc ccgctccaac ccgcgacggt
     6061 aggtatgggc gtggcagctg acgtccgccc gctcgcctag gcttgacgca tgagcgagcg
     6121 cacggcgtgg ggattcgggt tggccacggt gaccaagcac ggtgacgtgt tggacgcgtg
     6181 gttcccacag ccgtccttgg gcacgccgcc cgacgatgtc gccgcgccca ccgcgatcgc
     6241 cgaactcgcc ggcaaggaca aggtccgcaa ggtcacgcag gccgtggtgc tcgtcatcgc
     6301 cgaccttgac tcgccgcctg ccgacaccgc cgacgcctat ctgcgtctgc acctgctcag
     6361 ccaccggctc gtggcaccca actcgatcaa cctcgacggc atcttcgccg ccctgcccaa
     6421 cgtcgtctgg accaaccacg ggccgtgcgc ggtcgacggc ttcgagcaga cccgggcgcg
     6481 gctgcgcgcc caggggccgg tgacggtctt cggcgtcgac aagttccccc ggatggtcga
     6541 ctacgtcgtt ccgtcgggtg tgcggatcgc cgacgccgat cgggtccggc tgggtgccca
     6601 cctcgcctct ggcacgacgg tcatgcacga aggcttcgtc aacttcaacg ccggcacgct
     6661 aggcgcctcg atggtcgaag gccggatctc ccagggcgtc gtcgtcggcg acgggtccga
     6721 catcggcggc ggggcgtcga cgatgggcac cctgtccggc ggcggcaccg agcgggtccg
     6781 gatcgggcgg cgctgcctgc tcggcgcgga gtccggcctg ggcatcgcgc tcggcgacga
     6841 ttgtgtcgtc gaggccggcc tctatgtcac ggccggcacc aaggtcaccc tgcccgacgg
     6901 ccaggtcgtc aaggcgcgcg agttgtccgg ccaggacaac ctcctcttcc tgcgtgactc
     6961 ggtctcgggc acggtcgtcg tcaagtcgcg cgacggtcac gggatcgctc tcaacgccgc
     7021 gttgcacgcc aacgactgac cggacgaccc cgtatgccgc gcacccccgc gggcctgatc
     7081 ctcgccgaac aaggtccacc ctctcctcgt cgccgccggg tcgtgcgcgt gttcgtcgct
     7141 gccctcgtgc tcgtcgtggc cgctgctggc tgggtcgggg tgcgcacgat cctcgcgagc
     7201 ctgagcccgg aggcttgctg gacgacgtcg ccgtcggccc gggtcgaggt cacccccgag
     7261 caggccggca acgccgcgac catctccgcc gtcgccgtgt cccgcggcct gtccagccgc
     7321 gccgccacca tcgccatcgc gaccgccctg caggagtcga agcttcgcaa cctgcgctac
     7381 ggcgaccgcg attcggtcgg cctcttccag cagcgcccga gccagggctg gggcaccgtg
     7441 gagcagatcc tcgaccccgt ctatgcctcc aacgcgttct acaacaagct cgtcaaggtc
     7501 aaggactacc tcactcggcc gctcaccgag gtggctcagg aggtccagcg cagtggtgcg
     7561 ccagcggcat acgcccagca cgaggacgag gcacaggctc tcgccgccgc actgacgggc
     7621 gcggcgcctg cgaccttggt atgccgcctc gacccgccgt ccgccgtccc tgcggcgaag
     7681 gacctgaggg cggcgatcac caacgagttc ggcacgccga cggcggggtc cggcgggacg
     7741 gtcacggtgg ccgccgaggg ggccaccgcc tgggcgctgg cgtcgtgggc cgtcgcccac
     7801 gccgatgcgc gtggggtgag ccgcgtcgag gtgggcggcc ggacctggtc acggagcgac
     7861 ccgcagacct ggcagtcgac gccgagcggc cctcaggaca cggtcaccat caccctggtg
     7921 acggcgctct gaactcgacc ggcctggtgc gccggtcgag ggcccggcgt ggtcagcggt
     7981 agtcgatccc gacgtagtcc cgctcggtgg caccggtgta gatctgccgc ggccgcccga
     8041 tcttggtctc ggggtcagtc atcatctcgc gccactgggc gatccagccg ggcagccgcc
     8101 cgagcgcgaa caggaccgtg aacatcttgg tcggaaagcc catcgccttg tagatgagcc
     8161 ccgtgtagaa gtcgacgttg gggtaaagcc ggcgctcgac gaagtagtcg tccgcgaggg
     8221 cgatcgcctc caactcctgg gccaggccca gcagcgggtc gtccactccg agtttggcca
     8281 ggacgtcgtc agcggccttc ttgatcaacg aggctcgggg gtcgtagttc ttgtagaccc
     8341 ggtggccgaa gcccatcagt cggaccccgc cctccttgtt cttcactcga cgcatgaacg
     8401 cctcgggtga ctccccggac tcgcgaaggc tgttgagcat ctcaagcacg gcctggttgg
     8461 caccgccgtg caacgggccg aacaacgcgt tgatgccggc ggataccgac gcgaagaggt
     8521 tggcgtgcga cgagccgacc aaccggactg tcgaggtcga gcagttctgc tcgtggtcag
     8581 cgtgcaggag cagaaggagg tccagggcct tcgcgtggac cgggtcgacc tcgtagggct
     8641 cggcgggcgt gccgaaggtc atccgcagga agttctcggg cagcgacagg ttgttgtcgg
     8701 ggtagaggaa cggctgaccg atgctcttct tgtaggcgta ggcggcgatc gtcggcaact
     8761 tggccagcag acggatcgtc gagatctcga cctgcatcgg gtcgaacggg tcgagggagt
     8821 cctggtagaa ggtcgacagg gcgctcacgg ccgaggagag gaccggcatc gggtgggcat
     8881 cgcgcgggaa accggagaag aagttgcgca ggtcctcgtg caacatcgtg tggtgccgga
     8941 tccgcgagtc gaaatcggcg agttggctcg gcgtgggcag ctcaccgtag atgagcagga
     9001 aggccgcctc gacatagctg gacttctcgg ccaactgctc gatggggtag ccgcggtaac
     9061 gcaggatgcc gacgtcaccg tcgatgaagg tgatcgcgct gcggcaggct gccgtgttga
     9121 cgaagcccgc gtcataggtc acgaacccgg tgtctttgag cagcgcgccg acgttgaggc
     9181 cgggttctcc ctgggcggac ggcacgatct gcaggctcgc gtcacgagcg ccgacgtgaa
     9241 gcgaggcgtg ggtcgcggtc tcggtgtcgg tcatgaccat cccttcgtca ggtgaacatg
     9301 gctcatcccg gtggctcaac cggacgttac ctgagcacgt cagccctcgc gatgtgaccc
     9361 tacagacagg cgaccggcgg cagcggcgat gcgctcgtcg gtcgcagtga gcgccacgcg
     9421 cacatggcgg cgaccagcct caccgtagaa ggagcccgag ccgacgagga tcccacgctc
     9481 ggcgaaccag cccacggtgt cccagcagtc ctcgtcgcgg gtcgcccaga ggtagaggcc
     9541 cgcctctgaa tgctcgatcc gcagacccac gccctcaagc gcctcgatga ggatctctcg
     9601 ccgcacgccg tagcgcagcc gttggtcggc cacatgctcg tcgtcccgca gcgccaccgt
     9661 catcgctgcc tggacggggc caggcaccat catcccggcg tgcctgcgca cctcgacgag
     9721 gccggcgatg agggccgggt caccggccac gaacgccgcc cggtagccgg cgaggttgga
     9781 ctgcttggac agcgagtaga ccgcgaggag cccctcatgg ctgccgccgc acaccgcagg
     9841 gtccagcagg gagggggtcg tatgccgtgg cccggggcga cgggggtcgg gctgtcgcca
     9901 gtccaactcg gcatagcact cgtccgccgc gacgacgacg ccgtggcgac gtgcccacgc
     9961 cacgaccttc gccaggtgtt cgaccggcag gacggcgccc gtcgggttgc cggggctgtt
    10021 gagccagagc accttggggg tggtcgacgg ggtcagcggc ccaagagcgg ccaacccatc
    10081 gacgacgatc ggcgcggctc ccgccaaccg ggccccgacg tcgtaggtcg gatacgcgac
    10141 ggcagggatc ccgacaaggt cgccggctcc gaccccgagc aacgtcggca gccaggccac
    10201 caactccttg ctcccgatcg tcgggagcac cgcatcgggc gacagacccc ccactccgcg
    10261 gcgacgctcg aaccacgcga cgaccgcctc acgcagggcg ggtgtgccga cggtcgtcgg
    10321 gtagcccggc gagtcggccg ccgccacgag ggcagcctgg attgcctgcg gggtcgggtc
    10381 gaccggggtg ccgaccgaca ggtcgacgat gccgtcgcga tgcgcagcag cgatccgacg
    10441 gaacgggacg agcgagtccc agggaaagtc gggaaggctc ggaggcgtga gtggcagggc
    10501 gatcgctcag tgctcggagg cctgcggcgg cagcgcgctg accagggcgt ggtccttggg
    10561 gatcaggccc aacttggcgg caccgccggg gctgcccagg tcgtcgaaga actcgacgtt
    10621 ggccttgtag tagtcggacc actccgccgg gacgtcgtcc tcgtagtaga tcgcttcaac
    10681 ggggcagacc ggctcgcagg cgccgcagtc gacgcactcg tcggggtgga tgtagagcat
    10741 ccgctcgccc tcgtagatgc agtcgaccgg acattcctcg atgcaggcct tgtccttgag
    10801 atcgacgcat ggcagggcga tgacgtaggt catggtgagc ggtcctccta cgggcctggg
    10861 ttgacgcacc tagtatgccg agtcgacggg ggctgcacac ctgcgccccg gcgacgaagg
    10921 caggatatag ccgtgcggat cggcgtcagg gcactagaag atgtgatgac cgacgggtgg
    10981 cgggccctcg aggaggtctg gctcggcgac tggctgctgc gggcgtccca cggcttcacc
    11041 cagcggggca actccgcgct cgtcatcgac gaccccggcc tgccgctcgc cgccgcggtc
    11101 agcaccgtgg agcgttggta tgccgcccgc gacctgcccc cgcgcttctg tctggtcacg
    11161 gacgtggcgg ggtcagccgc cgacccctcg gtgtccaccc gcttggcgca gtcgggctac
    11221 gtgtcccgct cggcgaccta caccatgacg gcctttaccg ccgacctgcc gggcccgccc
    11281 acgggtgccc gtgacgtggt cgtcgacgac caggtcacgc cggcctggct ggcggcgttc
    11341 gcggcatacc gacgccttgt gccgggcgtc gccgaggcga tcctggccgg ctcgcacggt
    11401 caactgttcc tctcggtgcc cggcgagggc tccgccgatg ccccgccgat cgccatcgcg
    11461 cggatgtcga cctacccgca gtgggcgggc atccacgcga tgtgggtggc gccggagcac
    11521 cggcgtgagg gggtggccac ggtggtcgtc gccgcggtgg cccgcctcgc agccgagcgt
    11581 ggtctgccgg gggtctacct acaggtggag cgcagcaact cggtggccac ggcggcatac
    11641 gacggctggg gcttccgggc ccaccacggc cacgtctact acgcgccggc cgacgacggc
    11701 cttgggcaca gttagccggg cgcagctagc cggagcagat gatctggtcg gcgggaacgt
    11761 aggtcgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntag ccgggcgcag
    11881 ctagccggag cagatgatct ggtcggcggg aacgtaggtc gtcgagtagg actcgcgctt
    11941 gaccacggaa ccgcccttgg agaagacccg ctggacggtg atgtcgaagc cgtccgtgaa
    12001 ggcgtgcggc acgcacgacg ggctgctgtc ggtgatcgac ctgccgtgct tgatattggt
    12061 ccgggggccg gcgacggcgg tgacgtcgta gaccttcgtg ctccacatcc ggccgtgaac
    12121 ctggccgccg gacagccaca tctgcacgag gatgccgtag gggctgttgt tggtccactt
    12181 gttgtccagg tcgggccaac tcacggtggc ctcgcggccc gcggggtagc gcgagatgta
    12241 gaacgagtgc gccttgtgcg cggtgagctc gacgcccgcg aaccaggcga ggttgtaggt
    12301 caccgtcgag atctgcgaga caccgccacc aacgttcttg gtcaggcggt tgttggagat
    12361 gacgccggcc tcctggtagc ccttggccga cgtgcgctcc cccatcacct tgttgaggct
    12421 gaacacctca cccggcttga tgagcgtgcc gttgacggct gccgcgccga gggtcatgtt
    12481 gttggtccgg ggcgggtcgt aggggtaggc cgagtcgaag ctggcgatga cctcgttgac
    12541 ccccagggcc tgggcacctt cggtcgtcaa cgtcgccttg gccgcgaccg tcgacagcga
    12601 gacgcgccga tcgggggcgg tgagtgcctt caccaacagg tctgcggccg aggcggtgtc
    12661 gaccgacacc ccgtcgaccg aggggcggat gaccggcttg tcgccttcga ggacgatcgt
    12721 ggcgtccttg ggtgccacga cgacagcgct ggtcgccgac gcgacgacac cgcgcagggc
    12781 ctccttgtcg acgaccggat tgagcgtgcc gctctcgtcc gggaccatcg agagcgcctc
    12841 ggcatactgc tcgggcgtga cgactgcggt cttatcgccc acaacgatcg tcaccggggc
    12901 agacatcgcc ggcgccgcga acgaggtcac cgcggcatcg accttggcct tggtgacctt
    12961 cggtgcggtg atggacgcgg cagcggtgac cgactcgacc cgcggccagg cctggaccac
    13021 cttgcggacg gcatcgtcca gctgcagggc cacgccgtcg accgccgggg tggtggcgac
    13081 cttcccacca gcgaaagaca ccgtcccgtc gacggccgtg gtctggaccg tcgccgcctt
    13141 ctcggtgagc gcagccaaca gacggggctc gtcagccttg acgaggatcg gccggtcggt
    13201 gcgcccgctg aggcgggccc agatgacggt cggatcgagt gtgaagccgg tcagcccgtc
    13261 gagggttccg gggatgtcga aactcaaccc cgaggcgact ggatcgatgc tgaccgatga
    13321 ctgggtccct gccagatcga cccggatcgg agtcttggcc ttggctgcca actcggtcgt
    13381 gagtcgctcg gcggcctgct cggtcgacat gccaccgacg ctgaccccct cgacgacgag
    13441 cgcggcaggg acctgccgac cggtccacca ggcgaccgcg acgtacgccc ccgcgaggat
    13501 cgcgacggcc accacggacc ggacgacgat ccgactccac tcacgcacgc cgactctcct
    13561 tgccacgttg acccccgcta cgtccgcaca cggtagccca cgaacctgac accccgttga
    13621 atcgatcagc agtcagtcga gatgtccgta ctgcccacgg aagaacgtca gcgctgccgg
    13681 aggctcttcg gccaggctga gcgcgacgac ctcgccgacg acgatgctgt gatcgccgcc
    13741 gtcgtagacc gcagtcgtgc gcacctcgaa ccacgccagg gcatcgtcga gcaacgccac
    13801 gccggtcagc gaactgcgat ggtgcgcaac gggatccaac tgcccggcca gcggccgacc
    13861 acgcgtggca aaccaggcgg cagcgccccg cgcctgggcg ccgagcacgc tcacgcccca
    13921 gtgcccagcc gtgaggatcg cctcgtggaa ccgcgtctcg cgctcgacgc aaacgaggac
    13981 gaggggcggg gccagagaga ccgaggtcag ggagtttgcc gtcatcgcat ggtcggagcc
    14041 gtcgacgaga caggtgacga cgctcactcc ggtggcaaag cgcccgaatg cggtccggaa
    14101 ccgccccgga tcaaccggat cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 ncgttcacgg caggtccacc agcagaccct cgaaccaggt gtccgacgtc cgaggcagcc
    14281 gttcaccggt cgccggatcc aggcgcgcga acgcctctcg gccggacaac cgggcggcga
    14341 tgtcgttgga gaggctgtag tagccgtcga agagcgtcac ctgggtccgg tgagcagcca
    14401 acgcccaggt catccgtttc ccggccatct cgtcgacgac cacgtgactg acgacggcgt
    14461 cgtcgacgac ggacacggca tacggctcgt tctgggtggg gatgtgcagt ccgctcgcct
    14521 cgggcacctg ctcggcgagc cactgccgat cggcgaccac ccacgaccgc ggtgtcaggg
    14581 tctcgaagaa ccgtcccggc cgctcggccg gggccagccc ggtgatcgcg gcctgcacga
    14641 cccgccgggt ctggatgtgg tccgggtggc cgtagccacc gtctacgtca taggtcacca
    14701 cgacgtccgg gcgccgttcg cgcaggtatg ccgccaccag gcgtgcggcc tcggccacgt
    14761 cggccccggc gaaggcgcgc gggtggtcgg cggacggcat accggccatg cccgagtcgc
    14821 gccagcgcgg accggacccg tcatccccga ggaaggtatg ccgcgcgccc aggcgagcca
    14881 tcgcgccggc caactcgccc gcgcggaact ctccgagggt gtcgtcccgg tcggacgtgc
    14941 ggtggcgcac atcggccggg atcacctcgc cctcctcacc aagggtcgcg gtgaggacct
    15001 cgacatcgtg gccgcgcgag acgtagtggg cgatggctat gccggtggca agggtctcgt
    15061 cgtcgggatg tgcgtggacg aagagcagac gggccattac agaacagtac gtgcctcggg
    15121 gaagtggcag gccactcggt gacccgggct gagctccacg ggcacgggaa cctcgacggt
    15181 gcagcgttcc tgggccttcg ggcaacgggt gcggaagacg cacccgctcg gcggattctg
    15241 cgggctgggc aagtcgccct tgagcaggat ccgctccttg tgctcgatcc gcttggggtc
    15301 aggcaccggc acggccgaga gcagggcatg ggtgtagggg tgcaggggcg cctcgtagag
    15361 ctcgtcccgg tcggcctctt ccatgatctt gcccaggtac atgacgatga cccggtcaga
    15421 gatgtggcgg accaccgaga ggtcgtgcgc gatgaagacg taggccagac cctgctcctc
    15481 ctggatgtcc tccagaaggt tgacgacctg tgcctggatc gacacgtcga gggccgacac
    15541 gggttcgtcg cagacgatga gcttgggctt gagcgcgatc gcccgggcca ccccgatgcg
    15601 ctggcgctgc ccgccggaga actcgtgcgg gtaacggttg tagtgctcgg ggttgagccc
    15661 gacccgctcc atgaggccct ggacggccgc ccgacgacca ccctcggggg tgatcttctg
    15721 gatgtcgtag ggcgccgcga tgatcgagcc gatggtctgg cgcgggttga gcgagccgta
    15781 ggggtcctgg aagatcatct gcatctcgcg ccgcagcgtg cgcaactcgg cacccttggc
    15841 gtgggtgatg tcgcggccct cgaactcgat ggttccggcc gtcggctcaa gcagccgcag
    15901 cagggtccgc cccgccgtcg acttgccaca gccggactcc cccaccaggc cgaccgtctg
    15961 ccccggcgcg acgtcgaacg agatcccgtc gacggcacgc accgggttgt ccggtcgctt
    16021 gatgagggtc tggcggaact gcgggaagtg cttggtgaga ttggtcaacc gcaacagtgg
    16081 ccgacccgcc ggggtggccg acggtgcggc gccggtgctc gtctgaatgg tcatgcgccc
    16141 ctcctcacag cctgggcttg atctcgctgg cgaagagctc ggcgcgcgtg gccggctcca
    16201 gatggcagcg ggtcaggtga cccggaccgg ctgccgtcag gtcggggacc gccgctcgac
    16261 acgcatcggc aggcacctga ccggtgaagg cgcagcgcgg gtggaaggcg cagcccgacg
    16321 gcacgttgat gagcgagggc gggttccccg gaacgggatt gaggcgactg agccgggccc
    16381 ggtccaaccg aggcatcgac gtcagcagac cccacgtgta ggggtgctcg ggctcgtaga
    16441 acacgtcgtt gaccgggccg tgttcgacgg ccttgccgcc atacatgacg aggatgtcgt
    16501 cggccatctc ggcgaccacg ccgaggtcgt gggtgatcat gatgatcgcc gatccgaact
    16561 cagcctgcag tttgcgcatg aggtcgagga tctgggcctg caccgtcacg tcgagcgccg
    16621 tcgtcggctc gtcggcgatg agcaggctgg ggtcgttggc cagagccatc gcgatcatgg
    16681 cccgctggcg catcccaccg gagaactcgt gagggtattg gtcgacccgt cgggccgggt
    16741 tggggatgcc gaccagaccg agcaggtcga ccgccttggc cttcgcagcg gacttcgagg
    16801 tgtccgagtg atgcacccgg atcgcctcga cgatctgcga cccgatcgtg tagtaggggt
    16861 gcagcgccga gagcgggtct tggaagatca tcgccatctt gttgccgcgc aactcacgca
    16921 gccgctcgtc ggtgaccgtc atgaggtcct cgccgtcgag ccagatctgg ccggaggccc
    16981 gcgctcgggt gcgccggtgc aggccgagga tcccttggct ggtcaccgat ttgcccgagc
    17041 cggactcacc gacgatgccg atggtcttgc cccgctcgac cgtgaagttc agcccgtcga
    17101 cggccttgac cagaccgtca tcggtcggga agtgcacctt gaggtcgcgc acatcgaggt
    17161 atgccgtgcg ctcaccggcc gctctagcgg acggcgacgt ggtgacggtg gctccagagg
    17221 tgtcggccat ggtcagctca tcctcacgcg cgggtcgatg acgccgtaga ggacgtcgac
    17281 gacgaggttg gcgatgacta tgaagaacga ggccacgacg accaccgcaa cgatgacggg
    17341 aaggtcggtg ttggtgatgg cctggatcga cgtccagccc agtccgcgga agctgaagac
    17401 ctgctcgctg atgatggccc cgccgagcag accgcccagg tcgagaccgg cagcggtgac
    17461 gatcggggtg agcgctgcgc gcagcccgtg gcgccggacg acgaccgact ccttgacgcc
    17521 cttggctcgg gcggttcgga tgtagtcctc cgacatcgtc tcaagcatgt aggcgcgggt
    17581 gaggcggacg taggacgccc cgaagatggc ggcgagggtg agtgagggca ggaagatgct
    17641 ctgcatccac ggccagagtc cctcggagag ggggacgaag ttgggtatgg gccagagctg
    17701 ccacttgatg gcgatgaaga cgagcagcag ttggccgatg aagaagctcg gcagggagta
    17761 gccgatgagg gacacgccga cgagcagtcg gtcgatccag cgccctcgat tgagggccgc
    17821 gatgatcccg aagaagacac cggacgtgat ccacaggacg aaggcaccca gggcgatgga
    17881 ggccgtgatc ggcagaccgt ccttgatgat ctcggtcacc gacttggcct ggcgctgcga
    17941 gtaaccgagg cacggagcgg cgcaggtgaa cacggtctgc ggggcgcggg ccttctgctc
    18001 ggggtcgtcg gggaagtccc gggcgacgaa caggcccttg acgaagttgg cgtactgcac
    18061 ggtgatcggc ttgtcgaagc ccaagagctt gcggttgccc tcgatgaccg ccggcgtgca
    18121 gttcttcgcg caggtgaggc tcgccggatc gacgggcgag gcgaagaaca cgaggaatgt
    18181 ggtcatgctg atgatgaaga gcatgacgac gaccgagata agccgcctga tgatgtagaa
    18241 gaacatgggt cagggcacct cgcgggtgcg ctcaccttcc tctcggtccg gggggcgact
    18301 gtgccggacg ggggatgtcc ccatccggca cagtcggtag tcctgcgaca tcggtgccag
    18361 ccgtgtcagc accgacgtct gggtttactt aaccgcgatg ttcgccaggt cggcgtagcc
    18421 gccgaacagg gtgttgacct cgaagttctt gacgcccgaa ccgtagacga aggtgaactt
    18481 ctggttgacc agcgggatga cgccgccgtc cttggagatc atctcgtcga tgtcgcccca
    18541 cgccttctca cgggcggccg ggtcggtgat caggtatgcc gcgtccatgg ccttgttggc
    18601 cgcgtcgctg ttgtagtagc cgtagtcctg gttgctgctc gtcggcgtga tgttgatccg
    18661 accatcgaac agcggcggga tgtccgtcga gcccgacggc cagtcagcgc cccagctggc
    18721 gtagaaggca tccttggtct tcatggccgc cccggagatg gtgcggtagt acttgtcggt
    18781 gatcccctcg agggtcacgt tgaagcctgc cttgtcccac gtctccttca gcgccgcgaa
    18841 cgacttgtcg cgggtgggcg tcttgcggta gacgacggtg atgttgaccg gcatcgcgac
    18901 accagcgtcg gtgagaacct tcttcgcgcc gtcggggtca ccggcggtcg gagcaccgaa
    18961 ggggttgaag tccttgtagc acgcgagcac cttgttgcac atcgcgtagg tggggaccat
    19021 gacctgcggc ccaccgttgg cggtgatgta ggcgtcccgg ttggtcgcca tggcgaaggc
    19081 ctgacggacc ttggggttgg acatcgccgt gctcgtcatg ttcggctgga tgtagtcgac
    19141 gtacggagcc ggaacgttgg ccatgcgctt gtcggcgccc ggcgtgctgg cgatcgtcgg
    19201 cagcaccgac ggcggagccg taccgaaggt gatggcggtc ttgtcgtcgc caccgtcggc
    19261 cagcagtcgc tggtagaccg tctcgccctg gatgccctcg acgtcaacga tcttgtcggg
    19321 gtacgccttg cggatcgggt cggtcgccgc gtcccagttg gggttgcgga cccaggtgcc
    19381 acccttggcc gggtcgaagg tgccttccag catgtacggg ccgttggacc agggggcgaa
    19441 caggctcttg tcagccttgt cctgcgcctt ggggaacgga gcgaagatgc ccagcgccgc
    19501 cgtggcctgg ttgaagtcgg cccacggggt cttgaagtgc agcgtcagcg tctgaccgtc
    19561 acaggtgacg gctttgtcat agagcgcctg gccggtgccg ttgtacgggc ccttgtagga
    19621 cgacgtgccg tcggcgtcgt tcgggatgtc gaggaactgc agcgagtagg tcggcccgtt
    19681 ggagatcagg tcggtggcga aggtacgcga gccgccgtac ttaacgtcct cacaggtgac
    19741 cttggcgccg tcctgccact tggcgttgtc gacgatcgtg aacttccacg tcttgccgcc
    19801 gtcgctcatc gtgccgaggt cggtggcgag gtcaggcacc agcttggggt tcgctcccgt
    19861 cgagtagctg gtgaggtagc gcatgtagac ccggttggcg gtctgcaggt ccgcgccgac
    19921 gtaggactgc tgaacgtcca ggccgttgtg cgggccgagg ttcaggatgt agagcgtccc
    19981 gcccttcgcg actgccgcag ccgtcgacgc ggttgtggtg ctgggcgtcg agcccgagct
    20041 gcaggctgca gctgtcattg ccaccgccgc gccgagggcg acggcggccg cgcccttctt
    20101 catgcgcatc atgcaatctc cttgtcgtac ttccgtcgca cgtgctacga cgggtcgtcg
    20161 gtccgcgacg ggcgtcgcga accgtcactt atcgctctcg gggggatagc gataagtcgt
    20221 gggttctctg cgcaccgcgc agagggtatg ccggtcggcc caggctcatt gacggcccgc
    20281 cttggggtcg agcgcgtcgc ggaggccgtc gccgagaagg ttgaaggaca acacgaggag
    20341 gaacacggtc acccccggga agaagaagta ggccgggtcg ggcagcgcat aagagaccgt
    20401 gtcattgagg atcgcgccga gcgaggcagt cggcgggttg aggccgacgc cgaggaagcc
    20461 cagggccgct tcggccgaga tgttggccgg gaggacgagc gtggtgtaga cgagcagggg
    20521 ggcccacagg tgcgggagca actccttgaa gtagagccgg ccgcggcgtg ccccgagcga
    20581 gcgggccgcc tcaacgaact cgcgttcacg caacgacagc acctggccgc ggatgatccg
    20641 cgcgaagaac gggaagccga agaagccgag gacgagcacc atgaacgcga tcttcggtgg
    20701 tggagcgctc gacggctgac ccatgatcga ggcgatccgg tcgacgagca ccgcctgcaa
    20761 cgagagcagc atgagggtgc tggggaagga cagcacgagg tccatcgacc ggctgagcag
    20821 ccagtccacg cggcctccgg cgaagccgga gatgatgccg atgatcgttc cgacgacgac
    20881 cgagaagagc gtggcgagca gtgccacggt gagcgacgtc gtcataccgg tgacgatgcg
    20941 cgagagcagg tctcgcccgg tgccgggttc cactccgagc cagtgcgacc acgacacgcc
    21001 gcccatcagg ccttcgggca tcgagccgag gccttggacg agctcggggt gggtctggtc
    21061 ggggttgagg atgccgatcc tggcgagcag cggggcgatc gcagcgagcg cgacgaagaa
    21121 gacggaggcg ccaaacgcca ccatggtcac ccggtcacgg cgcaacctgc gcatgacgat
    21181 ctgccagggc gtccgcccgg cgatagcgac tgcgccgtcc accccgtcga gcgcgacggg
    21241 ggacccggga tcaacggcca cggccgaacc cacgggcacc gctgacgcgt ccgaacggtt
    21301 ctcgggttcg cccggggcca cagtcatcct caggtcgtcc ttccgtcgcg ttcggccatc
    21361 cgccggtgat ccttcaggtt tgggacaccc tagccggggc ccaacccaac aaaggaacaa
    21421 cttgaacgga gattctccgg atcgtgaccg aaccgcgatc gttcgtcagt gggcgacgga
    21481 cacccccgcc aggtcgaccc agccgcccag gtaggggttc tccgcgtagc ccccgacccc
    21541 ggacccgcgc aggaacgaac ggcgtcgctc ggccaacggg atctgcccga cgaccgcgac
    21601 gatcttcgcg tcaatcgcgg cccacgccaa ggcccgtttc acggggtcgg cctcgacctg
    21661 ggcgtccgcg atggcggtgt tgaccgcctc gtcgttgaag gaccccaggt cctgcccggt
    21721 cccgctgtcg gtgaggttca tccgcccgtc gaaaaggtcg ggaatgaccg ctgccccggc
    21781 cgggtagtcg gcgaaccagg acctgcggaa cacgtcgtag ttggccgagg cgtcaatccc
    21841 cgacacgatc cggtagtagt cctcccccag cccgtccaac gtgacctcga agcccacctt
    21901 ctcccacccg gccttgagcg cggcatacgc ggcatccgca gcaggggacg tgcgataggc
    21961 gacccggatg tgcacgggca gggtgacgcc ggcagcgacg agcgcagccc gcgcagcatc
    22021 ggggtcggcc gccgtcggga gcggagcagt ggagttcgcc gtggcagagg ccgtggcggt
    22081 cggcgtggcg tcggcggctg ggggcgtcgc cgggtgcccc ggaatggaac tggcgagcgc
    22141 agagtaggcg ggcgtcatca ccgtgctccc gtatgccgcg acgaacgcgg ctcggtcggt
    22201 cgccagggcc agtgcccgcc ggaccccctc gttgctcatc acggggctct tgacgttggg
    22261 ctgcagcaac tccaccgtgc ccgacaacgg attggagagc cgggccttca ccgctgggtc
    22321 ggcgagcaac gcggcgtgtc cggcggccgg aatgtcagtg ataccgatcg ccgtgtggtc
    22381 gggagccttg tcctcgacga gccgctggac cagcgtgttg acggggatcg actcgcggat
    22441 gtcgaccacg tcggggagcg cttggcgcaa cgcatctccc gcgcggttcc acgatgggtt
    22501 gcgcacgaac cgtccgcccg cccccgcctc ccacgcgccg tccaatcggt aagggccgca
    22561 ggagaatacg tcgaaagtcc ctgccccgcc gcggtcgtgg tcccgacggt atgccgcgaa
    22621 cgtcggcaag gcgacgatca cggggaagtc agggaccggc accttgaggc gcatcgtgag
    22681 ggtcgcgccc cgacaggaga ctgcagcatc gaattcggcc gacccgctgc cggactcnnn
    22741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntga cggcatacgg accgggatag
    22861 ccaggcacct cgagaccggc ggagtccacc cggctgggga tgtcgaggag cgtcatcggg
    22921 tagggggcgc cgccgggcag ttgctcgcgc gcgaacgacc gcgcgatgcc gtaggcgacg
    22981 tcctggcagg tgaccggttt cccgtcctcc cacgccggcc ccgagcgcag ggtgaacgtc
    23041 cacgtcttcc cgccgtcgga cggggtcccg gtgtcggtcg cgaggtcgcc gacgagccca
    23101 ccgactccac cggccccacc ggccgggtag ctcgtcaggg tcctgctgaa caggcgcacc
    23161 gcgatggcgc cctcgggacc ggagtacatc cgctgcgggt cccatgtggt gatcgtctgg
    23221 tcgtagagca gtcgcaacgt gccgcccgcg gcctccgtcg gggacggcga gttcggcgag
    23281 cccgtgcagg caccggtgag cagcacagac gcaaggaaca ggtatgccgc gcgcgcggca
    23341 tacctgggcg agcctgaccc tcgcatgagg gctagttttc ggccttggcg gctgaacggg
    23401 cacgggaggc cgtgcggcca cgttcgacct ggtcgaggac gaccttgcgg atgcggacgg
    23461 catcgggggt gacctcgacg cactcgtcct cgcggcagaa ctcgagggac tgctccaggc
    23521 tcaacttgcg ggccgggacg atcttctcga agttgtccga gctggaggcc cggatgttcg
    23581 tcagcttctt ttccttcgtg atgttgacgt ccatgtcgtc ggcgcgggag ttctcaccga
    23641 cgatcatgcc ttcgtagacc tcggtcgtcg gctcgacgaa gagcgtcccg cgctcctgca
    23701 ggttgaccat cgcgtaggcc gtcacggcac ccgagcggtc ggcgacgagc gagccgttga
    23761 tccgggtcgt gatcgggccg gcccaaggcg cgtagccctc gaacacgtgg tgggcgatgc
    23821 cggtgccacg ggtctcggtg aggaactcgg tccggaaacc gatgagcccg cgcgacggga
    23881 cgaggaactc catccgcacc cagccggtgc cgtggttggt catgtgctcc atctggccct
    23941 tgcgggcggc catgagctgg gtgaccacgc cgaggaactc ctcgggggtg tcgatggtga
    24001 ggcgctccat cggctcgtga accttgccgt cgacctgctt ggtgacgacc tgaggcttgc
    24061 cgacggtgag ctcgaagcct tcgcggcgca tctgctcgac gagaatggcg agggcgagct
    24121 cgccacgccc ctggacctcc caggtgtccg ggcggtcggt gttgaggacc cggatcgaga
    24181 c
//
LOCUS       DAORPW010000018        24150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_334_length_24150_cov_798.283544, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000018 DAORPW010000000
VERSION     DAORPW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 24150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24150
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_334_length_24150_cov_798.283544"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..413)
                     /locus_tag="PLB86_02970"
     CDS             complement(18..413)
                     /locus_tag="PLB86_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRC64126.1"
                     /translation="MIVDTSAVVAILLREPGSQALVDAILDAPSAKMSAGSALELQVV
                     ASRRGGDAGRVAALTILDDLSIDITAVTAQQALDAGAAHSRFGRGSGSKARLNFGDCF
                     AYALAAESDEPLLFVGDDFTHTDLTPVLP"
     gene            complement(410..691)
                     /locus_tag="PLB86_02975"
     CDS             complement(410..691)
                     /locus_tag="PLB86_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRC64127.1"
                     /translation="MALNIKNERVCRLAKEAAERTGRSQVSVLEAALEDYLAKVSAAD
                     AAKQESRAARVARVHATVEAYNVGLTDEDRAAVWRFMNDEMYDEDGLPA"
     gene            complement(759..1541)
                     /locus_tag="PLB86_02980"
     CDS             complement(759..1541)
                     /locus_tag="PLB86_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HRC64128.1"
                     /translation="MTDNATPRGEDWAGVVDDLNRLLRLKTTPVGLSRFATLAELEAV
                     PKVRRPDGIHTFDQVVAMAARLGWTVGATADDFVQKQCSAVLGLIPRDEEWLGGRALA
                     GVWFATIEDSAAHQRSMDTAPYAGHAGVVVSPLATGRLDPPDVVLIYATPGQMVLMIN
                     GLQWSGYRKLTMSSVGESACADSWGRALATGEPSVSIPCYAERRYGGVADDEMLMATP
                     PQFLPTMIAGLEALSRNGLRYPIAPYGIQADARAGLAKSYGN"
     gene            complement(1579..2277)
                     /locus_tag="PLB86_02985"
     CDS             complement(1579..2277)
                     /locus_tag="PLB86_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64129.1"
                     /translation="MQTWPEEPEVTDWQPVDDATLAGRLGALIPTSGAALVLIDGRSG
                     NGKSTLADRICRLIGAGLIHTDDVAWHLHATDWADALVGGVLEPWRRGDAVSFRPPGW
                     VARDRPGSIELAPTRILVVEGVGAGRAELARQADVVIWVQSDQAEARRRGVVRDVALG
                     RPPTEAEAFWDEWASSEDPFLAADRPWTRADFIVNGTPPRADDRTWLAPGPIGTATGP
                     LDPPNEPIGTSPVA"
     gene            2409..2753
                     /locus_tag="PLB86_02990"
     CDS             2409..2753
                     /locus_tag="PLB86_02990"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRC64130.1"
                     /translation="MAQYLFSVWHIGAYPTPSEEEMAAMFAAVGAFNDKMRDAGALVF
                     GCGIEDPASSVIVDGTGAEPTTRPGRFAAGPEILGGFWVIEAPSVEAAHELAAEASAA
                     CGVPLEIRPLQG"
     gene            2899..3489
                     /locus_tag="PLB86_02995"
     CDS             2899..3489
                     /locus_tag="PLB86_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005274927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HRC64131.1"
                     /translation="MTHIHGYTMGHVGAATAAAAVSGAELSQLRASVLFGDDDRAALR
                     RAGELLDDQIDALLDVWYGFVGSQPHLLAQFSNPSGAPIGDYLDKVRARFGQWVRDTC
                     AGDYGDDWLAYQHEIGRRHARVAKNTTDGADSTAYVPMRDLIALIVPITVTVRPFLAG
                     RANADDDVDAMMGAWLKAVVLSVALWTQPYVADGQW"
     gene            3483..4061
                     /locus_tag="PLB86_03000"
     CDS             3483..4061
                     /locus_tag="PLB86_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC64132.1"
                     /translation="MVAGVPSATITDALERLSQVQRVLLQEVSTAQGLSPIQVRILGL
                     VGAAAVRPSELAGRLGVTRATVTDAVRALEGKGLVAVATDPLDGRSRLLRVTEDGQAV
                     AGRVASWGMPVTHHLAALPSGDQGALLSGLLHLLTGLQADGLVPRARMCLTCRFFREA
                     SDAGGVAHCELLRADLPDTSLRLDCPDHEASG"
     gene            4200..5507
                     /locus_tag="PLB86_03005"
     CDS             4200..5507
                     /locus_tag="PLB86_03005"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRC64133.1"
                     /translation="MPAVVLVGAQWGDEGKGKATDLLGRRVDYVVKFNGGNNAGHTVV
                     IEQADGSTEKYALHLLPSGILTPSCTPVIGNGVVVDLEVLFREIDGLNARGVDTSKLI
                     VSADAHIITPYNRVLDKVTERFLGARKIGTTGRGIGPTYADKMSRTGIRVQDLFDRHI
                     LEQKVEAALLFKNQVLAKIYNRRAISVAEVVEELESYAARLAPMVRDTSLVLQEALDR
                     GETVLLEAGQATLLDVDFGTYPFVTSSNATAGGACTGSGIPPTRVARVIAILKAYTTR
                     VGEGPFPTELFDANGEFLRKVGTEYGTTTGRPRRCGWMDVVIGRYATRINGVTDFVVT
                     KLDVLTGLEEVPICVAYDVDGVRHNEMPVNQSDFHHAQPIYETMPGWWEDISGCRTFE
                     ELPANAQAYVLRVEELIGARVSAIGVGPGRDAIIVRHDLLGED"
     gene            complement(5586..7823)
                     /locus_tag="PLB86_03010"
     CDS             complement(5586..7823)
                     /locus_tag="PLB86_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRC64134.1"
                     /translation="MTTAHDLPQAPAGADDALAAELAIEQAHVDRVYAELDKASQRAR
                     LVAVEGLARGRTERTGEARDEELTGLFERDALVFHAARRQHALDTQYEGLVFGRLDLD
                     HPVAGRDSEALEREVRYIGRLGVRDDDYEPLVMDWRAPAAAPFYRATPVEPMGVLRRR
                     VLRCRGPVVIGAEDDLMVPEAPDGLVILGDGALMAALTRTRGARMRDIVATIQAHQDE
                     AIRAPGRGVTEITGGPGTGKTVVALHRAAYLLYADRRRYESGGILVVGPSAAYTAYIE
                     RVLPSLGEDSVALRALGDIVDAMSTDRHDTPAAAAVKGSLRMRELMARVARGLPDGVP
                     TELRTFVAGYAVRLDSTVLQRIRAHVLRQHPHNLGVEAARQTLAETALRLVGHPDRDD
                     FLGRFSDHIEVDAFLSAWWPQLDPREVLLWLTDRQRLDACAAGLLGPAEVDAVHASWM
                     EALETGTWSIADVALVDELAVRLGPEQEQPVEERGFFEIENLDELLEGATGGSFRPEE
                     VEPAYSTTPSDPRDRLLVGRIGRVADYAHVLVDEAQDLSPMQWRMLGRRGRRASWTVV
                     GDAAQSAWPDPVESLVARAEAWGGQEHRLFHMGTNYRNAREIFDYAAAVVRTEVPDAD
                     IPEAVRETGIPPREARLGERPLESIEAEAQRLLTEVDGSVAIITPRRYAAALASLDGS
                     AQGRLRVLDPLSTKGLEWDATLVVDPDEIVRESPGGIRTLYVVLTRAAHRMTVLQTRK
                     RGDDH"
     gene            7991..9283
                     /gene="purD"
                     /locus_tag="PLB86_03015"
     CDS             7991..9283
                     /gene="purD"
                     /locus_tag="PLB86_03015"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRC64135.1"
                     /translation="MKVLVIGSGAREHALVRGLSADPDVSAVVAAPGNPGIDLIAQCE
                     PLADVCDAAAVLAVAARHTVDLVVVGPEAPLVAGVSDALREAGYAVYGPSAAAAQLEG
                     SKSFAKEVMASAGVPTALAHVCTTMAEVEAALDALGAPHVVKDDGLAAGKGVVVTDDR
                     EAALAHARACLAKPDGQLVVEEFLDGPEVSVFCICDGTSVRALSPAQDFKRVGDGDLG
                     PNTGGMGAYSPLPWAPPHLSDWVIEHIAQPTIDEMHRRETPFVGTLYVGLAMTSRGPR
                     VVEFNARFGDPDGQVSLARLVTPLGTLLYAAATGRLDDADDLHHRPESAVVVVLAAEG
                     YPAAPVPGGTIEGLDAAAEVPGAWVLHAGTRRAEDGRIVAAGGRVLDVMAVGADLASA
                     RDTAYRAVSCLELAGGHHRSDIALAAARGEIHIPGSDT"
     gene            9280..10200
                     /locus_tag="PLB86_03020"
     CDS             9280..10200
                     /locus_tag="PLB86_03020"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRC64136.1"
                     /translation="MSDSTALVLPGYEHIYSGKVRDLYAPLDPVTGERRTDQVLLVAS
                     DRISAFDYVLDTPIPDKGRVLTQLSLWWFDRLVGLENHVVSTDVPAAVAGRAVLVRRL
                     EMIPVECIARAYLTGGGLSEYRQSGAVSGVPLPAGLEDGSRLPVPIFTPSTKAAMGQH
                     DEPMTIEEVQSAVGTQVAARIDVLTRRILARGNEIAGANGIIIADTKVEFGIDRSRED
                     DSGGPVIVLADEVLTPDSSRFWPADQWQPGRSQPSFDKQFVRDWLTSPESGWDRSSGE
                     APPPLPEHVVEATRARYLEAYERITGNTLP"
     gene            complement(10257..11450)
                     /locus_tag="PLB86_03025"
     CDS             complement(10257..11450)
                     /locus_tag="PLB86_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC64137.1"
                     /translation="MRDIGVGVISLGWMGRLHSTSYLAAPLRYSDLGVRTRLVHAADP
                     VPEAREYAAEQLGFARTSADYRELLADPEVDVVSICSPNFLHKEIALAAAAAGKPFWI
                     EKPMGVSADESADIARAAAAAGVVTSVGFNYRHAPAVARARQLIRDGALGRITNIRVW
                     LIADYASAPEGPLTWRYDRDRAGSGVVGDLMSHGLDLAQFLVGRVASVTALSSTFITE
                     RPIPTKVGVGHGGFEIGDERGPVGNEDYVGILARFDNGVVGTLESSRVSIGPRSEYAV
                     EVYGTGGSLRWNFEKINELQTCLVSATGNGPEHGYTTSLAGPQHGDFARFQPGPGVPM
                     GFDDLKVVEAQLFLRSVLTGQQLAPSAADGWSAAAAADAAVESAADGQWHAVPEVEGI
                     TTYDA"
     gene            complement(11493..13391)
                     /gene="iolD"
                     /locus_tag="PLB86_03030"
     CDS             complement(11493..13391)
                     /gene="iolD"
                     /locus_tag="PLB86_03030"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HRC64138.1"
                     /translation="MTETVRLTGAQALVRFLANQHSERDGVQQRLVTGMFGIFGHGNV
                     AGVGQALLQDQTAAEAGEPGEHLPYFLVRNEQGAVHAATAYSRTKNRLQTMAITTSIG
                     PGATNMVTGAALATTNRIPVLLLPSDQFATRYPDPVLQQLEDPGTLDVSVNDVFRPVS
                     RFFDRINRPEQLIPSLLNAMRVLTDPADTGAVTICLPQDVQAEAFDWPVALFRPRVWH
                     IGRPAPEAAALARAVEVIRSAKRPLVVAGGGVVYADGSEQLRAFATATGIPVADTHAG
                     KGAINWDHPSAVGGIGSTGTSAANALARQADVVIGIGTRYSDFTTASHTIFANPDVRF
                     VNINTLGFDAAKHSASMLVADAREALTALTGALDGWSVDAAYRQEAVDLDAAWQAVVE
                     ECYHRDQTPLPAQTEVFGALNELMGPEDVVINAAGSMPGDLQCLWRASTPVQYHVEYA
                     YSCMGYEIPAALGAKLALGDGHEVIAIVGDGTYQMLPQEIATIVSEGVKVIIVLLQNH
                     GFASIGGLSESRGSQRFGTNYRMRNRTSGRLDGEHVPFDLAANAASWGADVLRCATIA
                     DFRANYAAAAASDHTTVLYIETDLYGPNPPGSAWWDVPVSQVSDLQSTQTAYTEYVRE
                     QAAQRHYL"
     gene            complement(13455..14525)
                     /locus_tag="PLB86_03035"
     CDS             complement(13455..14525)
                     /locus_tag="PLB86_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRC64139.1"
                     /translation="MSDNASPLQTMTQTTTTALWNDSADPRELAQSIEWGAVGATCNP
                     VIAVTCVKADLPRWSARMREIAAERPTASESQIGWQVVEEISIEAARLLEPAFEKFKG
                     HNGRLSMQTDPRLHRDAVALADQAERFSTLAPNVIVKIPATHKGIAAIEDATARGVSM
                     NVTVSFTVPQAVAAAEAIERGLAAREAAGHDVSHMGPVVTIMVGRLDDWLKDVVKRDG
                     IDIDPSYLEWAGVAAFKHAYAIFQERGFRSRLLSAAFRNQLQWSEFVGGDVVISPPFG
                     WQQKIQASGADAAPRMDIPVDPTILDALLQIPEFRRAYEPDGMTTEEFDEFGATRKTL
                     RQFLAADEELDHLVREVILPAP"
     gene            complement(14711..15589)
                     /locus_tag="PLB86_03040"
     CDS             complement(14711..15589)
                     /locus_tag="PLB86_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRC64140.1"
                     /translation="MKIAQDPTPFHHDYRLLDFPRVVADLGYEHIQLTPHRDLIPFFR
                     HPKADDALVRAFKKACDDAGVGIASVLPVQRWSGPDEVAREAAVRNWKRIVQITVDLG
                     VSVIGTEFSGRPERSEESEAAFYKSMEELLPIIEREGIDVLIDPHPDDFVEDGLEAIR
                     VIRGLNSPNVGMVFVACHSYHMGHPMREIMREAGDLIRLVHVADTMDHHRSHGLRYIS
                     NPPGNTARVHQHLKIGDGDLDWDDFFGGLGEIGFYDRPGTVMCNSVFAEDETAGDMAR
                     FQLATMQSYVAKHHAG"
     gene            complement(15687..16424)
                     /locus_tag="PLB86_03045"
     CDS             complement(15687..16424)
                     /locus_tag="PLB86_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRC64141.1"
                     /translation="MHQIPVMIDRASPVPLYHQLAEQLTAAVEDGTLQPGDPFENELA
                     LADRLDLSRPTVRRAIAELVSRGLLVRRRGVGTAVASEVIHRRDELTSLFEDMERKGK
                     RPITRVLDIDRHSVTPRAAAALNLPQDTPLFYVERLRLAGDIPTALMHNWLPPQFADI
                     TEEELASGSLYAVLRARGVRPVVAHQTIGARRPRSAERKRLDLAPGDPLLTMTRRAYD
                     AAGTAIEFGDHCYRFDQYAFDVTVHER"
     gene            16576..17532
                     /gene="iolC"
                     /locus_tag="PLB86_03050"
     CDS             16576..17532
                     /gene="iolC"
                     /locus_tag="PLB86_03050"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HRC64142.1"
                     /translation="MAGPSFDVLCIGRVGVDLYPMEVGVGLEDVRTFGKFLGGSAGNV
                     AVAAARYGHSAAIISRTGDDPFGRFVATALGDLGVNTDYLGIDPWLPTPVTFCEIFPP
                     DHFPLYFYRYPQAPDLRVAPEQIPPVAVADSRVFWTTLTGLSAEPSRTAHATAWHLRG
                     RTAHTVLDLDYRPMFWVHESDASRAASDALEQSTVAIGNLEECRVAVGETDPDRAARA
                     LLDRGVDLAIVKQGPKGVLAMTADERVEAPPVWVEVVNGLGAGDAFGGAVCHGLLNGW
                     DLAETISFASAAGAYVAGRLECATAMPDEAEVRALQARAGRS"
     gene            17571..18419
                     /locus_tag="PLB86_03055"
     CDS             17571..18419
                     /locus_tag="PLB86_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRC64143.1"
                     /translation="MLEPDGIPAAWAARRAGAVPADGRLLVIAADHPARGALGVRDDR
                     RAMASRSDLLQRLAIAVSRPGVDGVLGTPDILEDLLLAGLLDGLLVYGSMNRAGLQGA
                     VFELDDRFTAYDAATIERLGLDGGKMLARVAYDDPATAPTLEACGRAVTDLAERGKIA
                     LVEPFLSSRSADGWVRNLLDPDSVITSVAIASGLGATSAHTWLKLPVVPEMERVLDST
                     TLPTLLLGGDPTNEPAQTYATWGRALACPPAVGLVVGRALLYPPGGDVAAAVDVAADL
                     VHGGAR"
     gene            18416..19297
                     /gene="iolB"
                     /locus_tag="PLB86_03060"
     CDS             18416..19297
                     /gene="iolB"
                     /locus_tag="PLB86_03060"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HRC64144.1"
                     /translation="MSDWLRPAGSSGDAEFDVRIAPGDPGWSHTGLYAVTLAAGESRT
                     VDTGDCEWIVLPLSGGSAVVEVGERVYFLTGRAHVFAGPTDLVYVPRDSRFTVTSTDG
                     GRIAFPFAKASRAFPVCRVDSSQVPVEMRGTGSSSRQVQNFGTPGVLEADSIIACEVI
                     TPAGNWSSYPPHKHDEDRDGVESALEEIYYVEVQREGGDGSAAGIAYLRNYGTESAPI
                     DTLAEVRSGDVVLIPHGWHGPAMAPPGYDLYYLNVMAGPGETRAWLICDDPAHAWVRG
                     AWVDAPVDARLPFTQVP"
     gene            19302..20306
                     /locus_tag="PLB86_03065"
     CDS             19302..20306
                     /locus_tag="PLB86_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC64145.1"
                     /translation="MRIGIVGVGRIGSFHAQTLAADPTVTDLVLLDPSATAAAAVASQ
                     LGATVAPDLDALLRRDLDALLVTSATHTHAEVLRAGVRAGLPTFCEKPVAASLDETIE
                     LARLEAASGVPIQVGFQRRFDAAYGRLRAAIASGELGFVHTIRAATHDQSPPPPGYLP
                     TSGGLFRDAAIHDYDVIRFVTGREVAQVYAAGANKGERFFTEAGDVDTAAALLTLDDD
                     TLVVTSVTRYNRAGHDIRMEVHGSLGALAAGLDDRYPMTSAEPGARFPAGPAHQSFLE
                     RFAAAYRAEMTEFVQVAAGAVPSRCTVADALEAFRVAEACGISRREGRPVALAEIPGA
                     "
     gene            20417..21916
                     /locus_tag="PLB86_03070"
     CDS             20417..21916
                     /locus_tag="PLB86_03070"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRC64146.1"
                     /translation="MSTPRRITHLIGHSEWDGTAERTGDVFNPATGEVTGKVDFASKA
                     LVDEVVANAAVAAAEWGRASLAKRTQVLFAFRQLLNDNKERVGALITAEHGKVTSDAI
                     GEVTRGLEIAEFACGIPHLLKGGFSENVSTNVDAYSILQPLGVVGVISPFNFPAMVPM
                     WFVPIAIACGNAVVIKPSEKDPSAVNAVAALWREAGLPDGVMSVVHGDKEAVDALLDN
                     TTVKAISFVGSTPIARYVYEKGTANGKRVQALGGAKNHMLVLPDADLDLAADAAVNAG
                     FGSAGERCMAISVLLAVDSIADELIAKISERMATLKTGDGSMGCDMGPIVTGVARDRI
                     VGYIEAGVGAGAELVVDGRETEFHGGADGFFVGPTLFDKVSTDMSIYTDEIFGPVLSV
                     VRVESYDEGLELINSSLYGNGTAIFTNDGGAARRFQNEVQVGMIGINVPIPVPMAYYS
                     FGGWKASLFGDSHAHGMEGVHFYTRTKAVTQRWLDPSHGGLNLGFPQNK"
     gene            22123..23055
                     /locus_tag="PLB86_03075"
     CDS             22123..23055
                     /locus_tag="PLB86_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRC64147.1"
                     /translation="MAESKARNTDPRYSKLTIGVCPDQWGVWFPQDDKQIDWDVALKE
                     MQTAGFSVMETGPFGYFPKDPTQLRDVMDEHGFTVVAGTGWGILHKAEAWNDTETFFR
                     AIGETHAAVGAKFVVHLPPMFRDEKSGAYTDDRTLSTEAWNLYIKNADKLGRIMKEDY
                     GLQMVLHPHGDSHIETPEDIDRIFQATDPEYVGFCLDTGHIVYGGGDPMELCRKYPER
                     ISYVHIKAMDQALVAQAHDEDWPFVKAVHAGCSVAPPAGLPDMGQLIEALADLDKELY
                     VVCEQDMYGCAKDYPFPNAVTTREYLASLGLGLK"
     gene            23077..24081
                     /locus_tag="PLB86_03080"
     CDS             23077..24081
                     /locus_tag="PLB86_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC64148.1"
                     /translation="MTLRIGMIGPGGMGQAHIERIHTVIAGGRVVAVSDVNAATAQAV
                     ADRIGATAYATSAELIASPDVDAVMICSHGPAHEPDVLACIAAGKPVFCEKPLTPTAD
                     AALRIMEAEQAGGRRLVTVGFMRRFDASYREMKAILDSGDLGEALMVHCAHRNPTVPD
                     FYTWDMAINDTAIHEIDTMRWLLGEEFVSARVDKPKKTSLRFEHLQDPLVLVLETASG
                     VRVDDEVFVNCQYGYDIRCELVAEKGAVSLADQNVVTRRDSVGRNNRISMNHNERFWG
                     AFVTEVQEWITAAAKGEHSGSTAWDGYAAACVCDAGVKALTDDGVVPVQMIAKPDFYA
                     "
BASE COUNT         3639 a         8380 c         8431 g         3700 t
ORIGIN      
        1 gacccagccg gggtgggcta ggggaggaca ggcgtgaggt cggtgtgggt gaaatcgtcg
       61 ccgacgaaca gcaacggctc gtctgactcc gcggccaacg cgtaggcgaa gcagtccccg
      121 aagttcaggc gggccttgct ccctgaaccc cggccgaatc ggctgtgggc ggccccggca
      181 tcgagggcct gctgcgcggt caccgcagta atgtcgatcg acaagtcgtc aagaatcgtg
      241 agggcggcca cgcggcctgc gtcgcccccg cgtcgactgg cgacgacctg caactccaga
      301 gccgaccccg ccgacatctt ggcgctgggg gcgtcaagaa tggcatccac cagggcttgc
      361 gagcccggct cgcgcaacag gatggcgacg acagccgagg tgtcgacgat catgcgggca
      421 acccgtcttc gtcgtacatc tcatcattca tgaagcgcca gacagctgcc cggtcctcgt
      481 cggtgagccc gacgttgtag gcctcgacgg ttgcgtgcac ccgggccacg cgtgccgctc
      541 ggctctcttg cttggcggca tcggcggccg acaccttcgc caaatagtct tcgagagcag
      601 cctccaggac gctgacctgg ctgcggcccg tgcgctcggc ggcctccttg gccaggcggc
      661 atacccgctc gttcttgatg ttgagcgcca tgcttacacc ctacacctgc atatgttaca
      721 ccttgagccc gaacagccct cgaaggtggg ctcgcgggtt agtttccgta gctcttggcc
      781 agccccgcgc gggcatcggc ctggatgccg tagggcgcga tcgggtagcg aagaccgttg
      841 cgggacaacg cttccaggcc cgcgatcatg gtcggcagaa actgcggcgg cgtcgccatg
      901 agcatctcgt cgtcagcaac cccgccatag cggcgctcgg cataacaggg gatcgacacc
      961 gacggctcgc cggtggccag cgcccggccc caggagtcgg cgcaggccga ctcgccgacc
     1021 gagctcatgg tgagtttgcg gtagcccgac cactgcagcc cgttgatcat gagcaccatc
     1081 tggcctggcg tggcatagat gagcacgacg tcgggtggat ctagccgccc cgtcgcgagg
     1141 ggagacacga cgacaccggc gtggccggca tacggggccg tgtccatcga gcgctggtgc
     1201 gccgcggagt cctcgatcgt cgcgaaccag acccccgcga gcgcgcgtcc gcccaaccac
     1261 tcctcgtcgc gcgggatcag accgagcacc gccgagcact gcttctggac gaagtcgtcg
     1321 gccgtcgcgc ccacggtcca cccgagccgc gccgccatcg cgaccacctg gtcaaacgtg
     1381 tgtatgccgt ccggccggcg aaccttcggc accgcctcca actcggcgag ggtcgcgaac
     1441 cgggagagcc ccactggggt ggtcttgagc cggagcaacc ggttgaggtc gtcgacgacc
     1501 ccggcccagt cctcgcctcg cggcgtcgcg ttgtcggtca tgctccgacg ctaatgcgcg
     1561 ggccaccatc gtggtccgtt aggcgacggg cgaggtgccg atcggctcgt tcggtgggtc
     1621 gagcggcccg gtcgccgtgc cgatcgggcc gggggcgagc caggtccggt cgtcggcgcg
     1681 tgggggagtc ccgttaacga tgaagtcggc ccgggtccac ggccgatcgg cggcgaggaa
     1741 ggggtcctcc gaactggccc actcgtccca gaaggcctca gcctccgtcg ggggccgacc
     1801 gagcgcaacg tcccgcacga cacctcgtcg gcgcgcctcg gcctgatccg attggaccca
     1861 gatgacgaca tcggcctgac gcgccaactc cgcccgaccg gcgccgaccc cctcgacgac
     1921 gagaatccgg gtgggcgcca gctcgatgga accgggccga tcacgggcca cccacccggg
     1981 cggccggaac gacacggcgt ccccgcggcg ccagggctcg agcaccccac cgacgagagc
     2041 gtcggcccag tccgtggcat ggaggtgcca ggccacgtcg tcggtgtgga tgagtccggc
     2101 tccgatcagg cggcagatcc ggtcggccag ggtcgacttc ccgttccccg accgtccgtc
     2161 gatgagcacc agggcagcac ccgaggtcgg gatcagcgcg ccgaggcgcc ctgcgagggt
     2221 cgcatcgtcg acgggctgcc agtcggtcac ctcaggttct tcgggccaag tctgcacccg
     2281 atggagtatg ccgccttgcc gcggggtcgt ccgtcgccac gcctggccac actggggagg
     2341 ctgctcgacc ggtgtcggtg ggacggcata ccgtgagggc gcactcaacc cgaggaaaga
     2401 ggcgacctat ggcgcagtac ctgttctccg tgtggcacat cggtgcctac ccgactccgt
     2461 ccgaggagga gatggccgca atgttcgcgg ccgtcggcgc cttcaacgac aagatgcgtg
     2521 acgcgggcgc gctggtcttc ggttgcggga tcgaagaccc ggcctcgtcg gtcatcgtcg
     2581 acggcacggg agccgagccc acgacgcgac cgggtcggtt cgctgccggc cccgagatcc
     2641 tcggcggctt ctgggtgatc gaggcaccga gcgtcgaggc ggcgcacgag ttggccgccg
     2701 aggcctccgc agcctgcggt gtcccactgg agatccgccc cctgcagggc tgaccgcaag
     2761 cagccgaacc gccccgacgg accacgacgg accacgacgg accacaacag accgcgcagc
     2821 tcgccgcgag gtctggacaa tcccttcagc atcagcaata gtgaggagtc cttagcaaca
     2881 tcgatcacag gaggaacgat gacgcacatc cacgggtaca ccatgggcca cgtcggggcc
     2941 gcaaccgcag cagcggcggt gtcgggcgcc gagctcagcc aactgcgcgc cagcgtcctg
     3001 ttcggcgacg acgatcgcgc ggcgctgcgc cgggccggcg agttgctcga cgaccagatc
     3061 gacgccctcc tggacgtttg gtacggcttc gtcggcagcc agccgcacct gctggcccag
     3121 ttcagcaacc cgtccggcgc ccccatcggc gactacctcg acaaggtccg tgctcgtttc
     3181 gggcagtggg tccgcgacac ctgcgcgggc gactacggcg acgactggct ggcctaccag
     3241 cacgagatcg gccgtcgcca cgcccgggtc gccaagaaca ccaccgacgg agccgactcc
     3301 acggcatacg tgccgatgcg tgacctcatc gcgctcatcg tgccgatcac cgtgacggtc
     3361 cgcccgttct tggcgggtcg cgctaacgcc gatgacgacg tggacgccat gatgggcgcg
     3421 tggctcaagg cggtcgtgct cagcgtcgcg ctgtggaccc agccctacgt cgcggacggg
     3481 cagtggtagc aggcgtcccc tccgcgacga tcaccgacgc cctggagcgg ctcagccagg
     3541 tgcagcgcgt cctccttcag gaggtgtcga ccgcacaggg gctgagcccg atccaggtgc
     3601 gcatccttgg gctcgtgggg gccgccgcgg tgcggccgtc cgagctggca gggcgcctcg
     3661 gggtcacccg agcgaccgtc accgatgccg tgcgggctct ggagggcaag ggcctcgtgg
     3721 ccgtcgcgac agaccccctc gacggccgca gccgcctgct gcgcgtcacc gaggacggcc
     3781 aggccgtcgc gggtcgcgtc gcgtcctggg gtatgccggt cactcaccat ctcgcggcgc
     3841 ttccctcggg cgaccagggg gcactgctct cgggcctcct gcacctgttg accggcctgc
     3901 aggcggacgg gctcgtgccg cgcgcccgga tgtgcctcac gtgtcggttc ttccgggaag
     3961 catccgacgc tggtggggtc gcgcactgcg agcttctccg ggccgacctg cccgacacct
     4021 ccctgcgcct ggactgcccg gaccacgaag cgtccggctg actgcggcga gcggggcgaa
     4081 cagggcgccc tgacgagagt gcggtgcacg gcatacggac gcggtgacct cagcaccacg
     4141 cgccccggta gcctgggatg tcgctccggc gaccacctcg cgaacggaag gacccgcgca
     4201 tgcccgcagt cgtcctcgtc ggcgcccagt ggggcgacga aggcaagggg aaagccactg
     4261 acctgctcgg tcgccgcgtc gactacgtcg tgaagttcaa cggcggcaac aacgccggtc
     4321 acacggtcgt catcgagcag gccgacggca gcaccgagaa gtacgcgctg cacctgctgc
     4381 catcgggcat cctcacgccg agctgcacgc cggtcatcgg caacggcgtc gtcgtcgacc
     4441 tcgaagtgct cttccgcgag atcgacggcc tcaatgcgcg tggagtcgac acctccaagc
     4501 tcatcgtcag cgccgacgcg cacatcatca cgccctacaa ccgggtgctc gacaaggtga
     4561 ccgagcgctt cctcggtgcc cgcaagatcg gcacgaccgg ccggggcatc ggccccacct
     4621 atgccgacaa gatgtcgcgc accggcatcc gggtccagga cctgttcgac cggcacatcc
     4681 tcgagcagaa ggtcgaggcg gcgctgctgt tcaagaacca ggtgctcgcc aagatctaca
     4741 accgacgggc catctcggtc gcggaggtgg tcgaggaact cgagtcgtat gccgctcggc
     4801 tggctcccat ggtccgcgac acgtcgttgg tgctccagga ggcgctcgac cggggcgaga
     4861 cggtgctgct ggaggccgga caagccacgc tgctggacgt cgacttcggg acctacccgt
     4921 tcgtcacctc gtccaacgcg acggccggcg gcgcctgcac cggctcgggc atcccgccga
     4981 cccgagtggc ccgcgtcatc gcgatcctca aggcctacac gacccgggtg ggggagggcc
     5041 ccttccccac cgagttgttc gacgccaacg gtgagttcct gcgcaaggtc ggcacggaat
     5101 acggcacgac gaccggccgg ccccgccgct gcgggtggat ggacgtggtc atcggccgct
     5161 atgccacccg gatcaacggg gtgaccgact tcgtcgtcac caagctcgac gtcctcaccg
     5221 gcctggagga ggtgccgatc tgcgtcgcct atgacgtcga cggcgtccgc cacaacgaga
     5281 tgccggtcaa ccagagcgac ttccaccacg ctcagccgat ctacgagacg atgcccggct
     5341 ggtgggagga catctccggc tgccgcacct tcgaggagct gccggccaac gcccaggcct
     5401 atgtcctgcg ggtcgaggag ctcatcggcg ctcgggtgtc cgcgattggc gtcggccccg
     5461 gccgtgacgc gatcatcgtg cgccacgatc tcctcggcga ggactgaggg tccgaccctc
     5521 gcgaggaggt atgccgccgc cccaccgcgc tgccctcgac tgaggcgctt ccgatcgggc
     5581 agacctcagt gatcgtcacc gcgtttgcgg gtttgaagca ccgtcatccg gtgtgccgcg
     5641 cgggtaagaa ccacataaag ggtgcgtatg ccgccgggag actcccgcac gatctcgtcg
     5701 gggtcgacga cgagggtcgc atcccattcc aaacccttgg tcgacagcgg gtcgagcacc
     5761 cgcagccggc cttgcgcgga gccgtccaac gacgccagcg ctgcggcata ccggcggggg
     5821 gtgatgatcg cgacgctgcc gtcgacctcg gtcagcagtc gttgagcctc ggcctcgatc
     5881 gactccaggg gccgctcgcc gaggcgcgcc tcacgcggag gtatgccggt ctcgcgcacc
     5941 gcctcgggga tgtcggcgtc gggcacctcg gtccgcacga cggctgccgc gtagtcgaag
     6001 atctcgcggg cgttgcggta gttggtgccc atgtggaaga gccggtgctc ctgcccaccc
     6061 caggcctcgg cacgggcgac gagcgactcg accgggtcgg gccatgcgct ctgggcggca
     6121 tcgccgacga cggtccagga agcccggcgg ccgcgccgcc cgagcatccg ccactgcatc
     6181 ggcgacaggt cctgcgcctc gtcgacgagc acgtgggcat agtcggccac ccgcccgatg
     6241 cgtccgacga gcagccgatc gcgcggatct gagggagtcg tcgagtaggc cggctcgacc
     6301 tcctccggac ggaacgaccc gcccgtggcg ccttcgagga gttcgtcgag gttctcgatc
     6361 tcgaagaagc cgcgctcctc gacgggctgc tcctgctcgg ggcccaggcg caccgcgagt
     6421 tcgtcgacga gcgcgacgtc ggcgatcgac caggtcccgg tctcaagggc ctccatccag
     6481 gaggcatgga ccgcgtcgac ctcggcgggg ccaaggagcc cggccgcgca ggcgtccaga
     6541 cgctggcgat cggtcagcca gaggagcacc tcgcgcgggt ccagttgcgg ccaccaggcc
     6601 gagaggaatg cgtcgacctc gatgtggtcg ctgaagcggc cgaggaagtc gtcgcggtcg
     6661 gggtgaccga cgagccgcag ggccgtctcg gcgagggtct ggcgggccgc ctcgaccccg
     6721 aggttgtgcg ggtgttggcg caggacgtgc gcgcggatcc gttgcaggac agtcgagtcc
     6781 aggcgcacgg catacccggc gacgaaggtg cgcagctcgg tggggacgcc gtcgggcagg
     6841 ccgcgcgcca cccgcgccat gagctcgcgc atccgcagcg accccttgac cgcggccgcg
     6901 gccggggtgt cgtgccggtc ggtcgacatc gcgtcgacga tgtcgcccag ggcgcgcagc
     6961 gcgacgctgt cctcaccgag ggagggcagg acccgctcga tgtatgccgt gtaggccgcc
     7021 gagggcccga ccacgaggat gccgccgctc tcgtaacgcc gccggtcggc atacaacagg
     7081 tatgccgcgc ggtgcagcgc gacgacggtc ttgccggtgc cggggcctcc ggtgatctcg
     7141 gtgacgccgc gcccgggagc ccggatcgcc tcgtcctggt gggcctggat cgtcgcgacg
     7201 atgtcgcgca tccgggcgcc gcgggtgcgg gtgagcgccg ccatgagcgc gccgtccccg
     7261 aggatgacca acccgtcagg ggcctcgggg accatgaggt cgtcctcggc gccgatgacg
     7321 acgggcccgc ggcagcgcag gacacgccga cgcaggacac ccatgggctc gacgggcgtc
     7381 gcccgataaa acggagcggc ggcgggtgcg cgccagtcca tgacgagcgg ctcgtagtcg
     7441 tcgtcgcgga ccccgagtcg cccgatgtaa cggacctcgc gttcgagcgc ctcgctgtca
     7501 cggccggcca cggggtggtc gaggtccagg cggccgaaga ccaacccttc gtactgagtg
     7561 tccagggcat gctgacgacg agcggcgtgg aagacgaggg cgtcgcgttc gaacagcccg
     7621 gtgagctcct cgtcccgggc ctcgccggtg cgctcggtgc ggccacgagc caggccttcg
     7681 acggccacga gtcgtgcgcg ctgggacgcc ttgtcgagtt cggcgtaaac acggtcgacg
     7741 tgggcctgct cgatcgcaag ctctgccgcg agggcgtcgt cggcccccgc gggcgcttgg
     7801 ggcaggtcgt gtgccgtcgt catcgagcac ccgtcctggt cacggcgtcc atccggtcgc
     7861 aggtttccgt cccgtcgttt cgagaggccg gtcgcccggc cacggaacgc acaagtctag
     7921 tgcagcggcg gccgctcgcg cgcccgccca gcccgggcga gacggcggcc cggggcccta
     7981 gggttggggt gtgaaggtcc tcgtcatcgg gtccggtgcc cgcgaacacg ccctcgtccg
     8041 cggcctgagc gccgatcccg acgtatcggc cgtcgtcgcc gccccgggca accccgggat
     8101 cgacctcatc gcccagtgcg agccgctcgc cgacgtgtgc gatgccgccg ccgtgctcgc
     8161 ggtggctgcg cggcataccg tcgatctggt cgtcgtgggc cccgaggcgc ccctggtcgc
     8221 gggagtctcc gacgccttgc gcgaggcggg gtatgccgtc tacggcccgt ccgccgcggc
     8281 ggcccagttg gagggcagca agtccttcgc caaggaggtc atggcctcgg caggggtgcc
     8341 caccgcattg gcccatgtgt gcacgacgat ggccgaggtc gaggccgccc tcgatgcgct
     8401 cggggcgccg cacgtcgtca aggacgacgg gctggccgcc ggcaagggcg tcgtcgtcac
     8461 ggacgaccgc gaggccgccc tggcccacgc gcgcgcctgc ctggccaagc cggatggtca
     8521 actggtcgtc gaggagttcc tcgacgggcc cgaggtgtcg gtcttctgca tctgcgacgg
     8581 caccagcgtg cgggcgttgt cacccgccca agacttcaag cgggtcggcg acggtgacct
     8641 cgggcccaac accggtggca tgggcgccta ttcgccgttg ccctgggcgc cgccgcacct
     8701 gtccgactgg gtcatcgagc acatcgccca gccgacgatc gacgagatgc accgccggga
     8761 gaccccgttt gtcggcaccc tctacgtcgg cctggcgatg acgtcgcgcg gcccacgggt
     8821 ggtggagttc aacgcgcggt tcggcgaccc cgacgggcag gtctcgctcg ctcggttggt
     8881 cacgcccctg ggcacgctcc tgtatgccgc cgcgaccggc cgcctcgacg acgcggacga
     8941 cctgcaccac cgacccgagt cggcggtcgt cgtcgtgctc gctgcggagg gctaccccgc
     9001 cgcgccggtg cccggcggca cgatcgaggg cctcgatgcg gcggcggagg tgcccggggc
     9061 ctgggtgctt cacgcgggca cgcgccgggc cgaggacggc cggatcgtcg cggccggcgg
     9121 ccgggtgttg gacgtcatgg ccgtgggtgc cgacctggca tcggcgaggg acacggcata
     9181 ccgcgcggtg tcctgccttg agttggccgg tgggcaccat cgcagcgaca tcgcgcttgc
     9241 tgcggcgcga ggcgagatcc acatcccagg gagtgacaca tgagcgactc gacggccctc
     9301 gtcctgccgg gctatgagca catctattcg gggaaggtcc gcgacctgta tgcgccgctc
     9361 gatccggtga cgggtgagcg ccggaccgac caggtgctgc tcgtcgcgtc cgaccggatc
     9421 tcggcgttcg actacgtcct ggacaccccc attcccgaca aggggcgggt gctgacgcag
     9481 ttgtcgctgt ggtggttcga ccggctcgtg ggcctggaga accatgtggt ctcgaccgac
     9541 gtgcccgccg cggtggccgg ccgcgccgtt ctggtgcggc gcctggagat gatccccgtc
     9601 gagtgcatcg cccgggccta tctcaccggc ggcggcctga gcgagtatcg gcagtccggg
     9661 gcggtgtctg gcgtcccgct tccggccggg ctcgaggacg gctcgcggct gccggtgccg
     9721 atcttcacgc ccagcaccaa ggccgcgatg ggccagcacg acgagccgat gaccatcgag
     9781 gaggttcaga gcgcggtcgg gacccaggtg gcggcccgca tcgacgtctt gacccgccga
     9841 atcctggccc gtggcaacga gatcgccggc gccaacggca tcatcatcgc ggacaccaaa
     9901 gtggagttcg gcatcgaccg ctcccgcgag gacgacagcg gcggccccgt catcgtgctg
     9961 gccgacgagg tcctcacccc cgactcgtca cgcttctggc cggccgatca gtggcagccg
    10021 ggccggtcgc agccgtcgtt cgacaagcag ttcgtccgcg actggctgac ctcgccggag
    10081 agcggttggg accgttcgtc gggcgaagcc ccgccgccct tgcccgaaca cgtcgtcgag
    10141 gcgacccggg cgcggtatct cgaggcctac gagcggatca ccgggaacac gcttccctga
    10201 cgggtctgcc ggctctgacc gacgagtctg ccgggtctga cgggtatgcc gcgtggtcag
    10261 gcgtcgtagg tggtgatgcc ctcaacctcg ggcacggcgt gccactggcc gtcggcggcc
    10321 gactcgactg cggcatcggc ggcggctgca gccgaccagc catcggcagc cgacggtgcc
    10381 aactgctgac cggtgagcac cgagcgcagg aagagctggg cctcgaccac cttgaggtcg
    10441 tcgaatccca tcgggacgcc gggaccgggc tggaagcggg cgaagtcgcc gtgctgcggc
    10501 ccggccagcg acgtcgtgta gccgtgctca ggcccgttgc cggtggcgct gaccaggcag
    10561 gtctgcagtt cgttgatctt ctcgaagttc cagcgcagcg atccgcccgt gccgtagacc
    10621 tcgacggcat actccgagcg cgggccgatc gacacgcgtg aggattccag cgtgccgacg
    10681 acgccgttgt cgaagcgagc gaggatgccg acgtagtcct cgttgccgac gggtccgcgc
    10741 tcgtcgccga tctcgaaacc gccgtgcccg acgcccacct tggtggggat cgggcgctcg
    10801 gtgatgaacg tcgagctcag cgcggtgacc gaggcgaccc ggccgacgag gaactgggcg
    10861 aggtccagcc cgtgcgacat gaggtcaccg acgaccccgg agccggctcg gtcccggtcg
    10921 tagcgccagg tgaggggccc ttcgggtgcc gacgcatagt cggcgatcag ccagacccgg
    10981 atgttggtga tccggccgag ggcaccgtcg cggatgagct gccgggcgcg ggcgaccgcc
    11041 ggcgcgtgcc ggtagttgaa cccgaccgag gtgaccactc cggccgcggc ggcggcgcgg
    11101 gcgatgtcgg cgctctcgtc ggcgctgacg cccatcggct tctcgatcca gaacggcttg
    11161 ccggccgcgg ccgccgcgag ggcgatctcc ttgtgcagga agttcggcga gcagatcgac
    11221 acgacatcga cctcggggtc ggcaagcaac tcgcggtagt ccgcgctggt gcgggcgaaa
    11281 ccgagctgct cagcggcata ctcccgcgcc tccggcaccg ggtcggcagc gtggacgagg
    11341 cgcgtccgca ctccgaggtc ggagtaacgc agcggagcgg cgaggtagga cgtcgagtgc
    11401 aaccggccca tccagccgag gctgatgacg ccgactccga tgtcacgcat gaggattcgt
    11461 tcccttgtgt ctaggcgggg cagccggtcg ggtcacaggt agtgacgctg ggcggcctgc
    11521 tcgcggacgt actcggtgta tgccgtctgg gtgctctgca ggtcgctcac ctgcgagacc
    11581 ggcacgtccc accacgctga gccgggcggg ttggggccat agaggtcggt ctcgatatag
    11641 agcaccgtcg tgtggtcgga ggcggccgcc gcggcgtagt tggcccggaa gtcggcaatg
    11701 gtcgcacatc gcagcacgtc ggcaccccag ctcgcggcgt tggccgcgag gtcgaagggg
    11761 acatgctcgc cgtccaagcg tccagaggtc cggttgcgca tccggtagtt ggtgccgaag
    11821 cgctgcgagc ctcgcgactc tgacagcccg ccgatcgacg cgaagccgtg gttctgcagc
    11881 aggacgatga tgaccttgac gccctcggag acgatcgtcg cgatctcctg cggaagcatc
    11941 tggtaggtgc cgtcaccgac gatcgcgatg acctcgtgcc cgtcaccgag ggccaacttg
    12001 gcgcccaggg cggccgggat ctcgtatccc atgcaggagt aggcgtattc gacgtggtac
    12061 tgcacggggg tcgatgcgcg ccacaggcat tgcaggtcac cgggcatcga gccggcggcg
    12121 ttgatgacga cgtcctcggg ccccatcaac tcgttgaggg ctccgaagac ctcagtctgg
    12181 gcgggcagcg gggtctggtc gcggtggtag cactcctcca cgaccgcttg ccacgcggca
    12241 tcgaggtcga cggcttcctg ccggtatgcc gcgtcgacgc tccacccgtc cagcgccccg
    12301 gtgagcgccg tgagggcttc gcgggcgtcg gcgacgagca tgctcgccga gtgcttggcc
    12361 gcgtcgaagc cgagggtgtt gatgttgacg aaccgcacgt cggggttggc gaagatcgtg
    12421 tggctggcgg tcgtgaagtc ggagtaccga gtgccgatgc cgatgaccac atctgcctgt
    12481 cgcgcaagcg cattcgcggc cgaggtgccg gtcgagccga tcccgccgac ggcgctcggg
    12541 tggtcccagt tgatggcgcc cttgcccgca tgggtgtcgg ccacggggat gccggtcgcg
    12601 gtggcgaacg cgcgcaactg ctcggaccca tcggcataca cgacaccgcc gccggcgacc
    12661 acgagcggcc gctttgccga tctgatgacc tcgaccgcgc gggcgagcgc tgcggcctca
    12721 ggggccgggc ggccgatgtg ccagacccgc gggcggaaca gtgccacggg ccagtcgaac
    12781 gcctcggcct gcacgtcctg ggggaggcag atcgtcactg ccccggtgtc ggccgggtcg
    12841 gtgagcaccc gcatcgcgtt gagcaacgac gggatgagct gctcggggcg gttgatccgg
    12901 tcgaagaagc gcgacacggg acggaagacg tcattgaccg acacgtcgag ggtgccgggg
    12961 tcctccaact gctggagcac cgggtcggga tagcgcgtcg cgaactggtc ggagggcagc
    13021 aggagcaccg ggatccggtt ggtcgtcgcg agcgcagcgc cggtgaccat gttggtcgca
    13081 ccggggccga tcgacgtggt gatggccatc gtctgcaggc ggttcttggt gcgggagtag
    13141 gcggttgccg cgtggacggc gccctgctcg ttgcggacca ggaagtaggg caggtgctcg
    13201 ccgggctccc cggcctcggc ggcggtctgg tcctgcagaa gcgcctgccc gacgccggcc
    13261 acgttgccgt gcccgaagat gccgaacatg cctgtgacga gccgctgttg gaccccgtca
    13321 cgctcggagt gctggttggc caggaaccgc accagggcct gggcccccgt cagccgcacc
    13381 gtctcggtca tggtcgtcgt ccttctcacg tggtcggggt ggtgctcatt cggagggtat
    13441 gccgcgggct cgcctcacgg ggcggggagg atgacctcgc ggaccaggtg gtcgagctct
    13501 tcgtcagcgg cgaggaactg gcgcagcgtc ttgcgggtcg cgccgaactc gtcgaactcc
    13561 tccgtcgtca tgccgtcggg ctcgtaggcg cgacggaact ccgggatctg caggagggcg
    13621 tcgagaatgg tcgggtccac cgggatgtcc atgcggggcg ccgcgtccgc gccgctggcc
    13681 tggatcttct gctgccagcc gaacggcggg gagatgacga cgtctccgcc gacaaactcg
    13741 ctccactgca actggttacg gaaggccgcc gagagcagcc gggaccggaa gccacgctcc
    13801 tggaagatcg cgtaggcgtg cttgaaggcc gcgaccccgg cccactcgag gtagctgggg
    13861 tcgatgtcga tcccgtcccg cttgacgacg tccttgagcc agtcgtccag gcgcccgacc
    13921 atgatcgtca cgacggggcc catgtgcgac acgtcgtggc ccgccgcctc ccgggcagcc
    13981 aacccgcgct cgatcgcctc ggccgccgcg acggcctgtg ggacggtgaa ggacacggtg
    14041 acgttcatgc tgacgccgcg tgccgtggcg tcctcgatgg cggcaattcc cttgtgggtc
    14101 gcgggaatct tgacaatgac gttgggcgcc agggtggaga accgctcggc ttggtcggcc
    14161 agagcaaccg cgtcgcggtg cagccgcggg tcggtctgca tcgagagccg accgttgtgg
    14221 cccttgaact tctcgaaggc cggctcgagc agcctggcgg cctcgatgga gatctcttcg
    14281 acgacctgcc acccgatctg cgactcagac gcggtcgggc gctcggcggc gatctcgcgc
    14341 atccgcgccg accagcgggg caggtcggcc ttcacgcagg tgaccgcgat gaccgggtta
    14401 cacgtggccc cgacggcccc ccactcgatg gactgggcga gctcgcgtgg gtcggccgag
    14461 tcgttccaga gcgcggtggt cgtcgtctgg gtcatcgtct gcagtggcga tgcgttgtct
    14521 gacatgggtt cctccggggc gggcggcgct ggtggtccga gacagcggtg tcgcggcgaa
    14581 ctcggtgctg gtgaagctcg cggctggtga gtcgagacta gctctcgccc tgcctgccgt
    14641 gtcaatagct tgtcctaaca ttctcgcaaa ttgttagcgc gaggatggac cgctgtgcgg
    14701 tgcgagcagg tcagccggcg tggtgcttgg cgacgtagct ctgcatcgtc gcgagctgaa
    14761 agcgcgccat gtcgccggcc gtctcgtcct cggcaaagac gctgttgcac atgactgttc
    14821 ccggtcggtc gtagaagccg atctcgccca acccgccgaa gaagtcgtcc cagtcgaggt
    14881 cgccgtcgcc gatcttgagg tgctggtgga cccgggcggt gttgccgggc gggttggaga
    14941 tgtagcgcaa cccatgcgaa cggtgatggt ccatcgtgtc ggcgacgtgg acgagccgga
    15001 tgagatcgcc ggcctcgcgc atgatctccc gcatcgggtg acccatgtgg tagctgtggc
    15061 aggcgacgaa gaccatgccg acgttggggc tgttgagccc ccggatcacg cggatggcct
    15121 cgaggccgtc ctcgacgaag tcgtcggggt gcgggtcgat gagcacgtcg atgccctcgc
    15181 gctcgatgat cgggagcaac tcctccatcg acttgtagaa ggctgcctcg gactcctcgg
    15241 agcgctctgg gcgtccggag aactccgtgc cgatcacgct cacgccgagg tcgacggtga
    15301 tctggacgat ccgcttccag ttgcgcacgg ccgcctcccg ggcgacctcg tccgggccgg
    15361 accagcgctg cacgggaagc accgaggcga tgcccacgcc ggcgtcgtcg caggccttct
    15421 tgaaggcgcg caccagggcg tcgtcggcct tcgggtggcg gaagaacggg atgaggtcgc
    15481 ggtgcggggt cagttggatg tgctcgtagc cgaggtcggc caccacccgc gggaagtcga
    15541 gcaggcggta gtcgtggtgg aacggagtcg gatcctgggc gatcttcatg gtgcctcctc
    15601 tggcgagcct gacgtcacta tgtcaggaca ttagcgccag tgcgcccgaa gcgccatccc
    15661 cagctttgaa ctagcgctcg tgactctcag cgctcgtgaa cggtgacatc aaacgcgtac
    15721 tgatcgaacc ggtagcagtg gtcaccgaat tcgatcgccg tgccggcggc gtcgtaggcg
    15781 cgacgggtca tcgtcagcag tgggtcgccc ggcgccaggt ccagccgctt gcgttcggcg
    15841 cttcgcggac ggcgggcccc gatcgtctgg tgggcgacga cggggcggac cccgcgagcc
    15901 cgcaacacgg catacaggct gccggaggcg agctcctcct cggtgatgtc ggcgaactgc
    15961 ggcgggagcc agttgtgcat gagcgccgtg gggatgtcgc ccgccaggcg caaccgctcg
    16021 acatagaaca gcggcgtgtc ctgcggcagg ttcaaagccg ccgcagcccg gggggtgacc
    16081 gagtggcggt cgatgtccag cactcgcgtg atgggacgct tgcccttgcg ctccatgtct
    16141 tcgaacaggc tcgtgagctc gtcgcggcgg tggatgacct cgctggccac ggccgtcccg
    16201 acgccgcggc gccgcaccaa caacccgcgc gacaccaact cggcgatcgc ccggcgaacc
    16261 gtcggccgag agagatccag ccgatcggcg agggcgagct cgttctcgaa cggatcgccc
    16321 ggctgcaacg tgccgtcctc gacggcagcg gtgagctgct cggcgagctg gtgatagagc
    16381 gggacggggc tcgcccggtc gatcatcacg gggatctggt gcacgaaacc gagcatatgt
    16441 caggaacagt gttttgtcgt ggatcagcaa ttgtcctaac aaacattgac aacgcagggg
    16501 tcggtccggc accctgggaa agccagcgca gcgacgcacc cagcgacaac gacagcccag
    16561 gaggtggacc cgatgatggc cggcccgtcc ttcgacgtgt tgtgcatcgg aagggtcggt
    16621 gtcgaccttt accccatgga ggtcggagtc ggtctggaag acgtccgcac cttcgggaag
    16681 ttcctcggtg gcagtgccgg caacgtcgct gtggcggctg ctcggtacgg tcactcggca
    16741 gcgatcatca gccgcaccgg cgacgacccc ttcggtcggt tcgtcgcgac ggccctgggc
    16801 gacctgggtg tcaacaccga ctacctcggg atcgacccgt ggctgcccac cccggtgacc
    16861 ttctgcgaga tcttcccgcc ggaccatttc ccgctctact tctaccgcta cccgcaggcc
    16921 cctgaccttc gcgtcgcccc cgaacagatc ccgccggtgg cggtggccga cagtcgggtc
    16981 ttctggacga cgctcaccgg cctctcggca gagccaagcc ggacggccca cgcgaccgcg
    17041 tggcacctgc gcggacgcac ggcgcacacc gtcctcgacc tcgactaccg gccgatgttc
    17101 tgggtccacg agtctgatgc gagtcgcgcg gctagcgacg cgcttgagca atcgacggtg
    17161 gccatcggca acctcgagga atgccgagtg gcggtcggag agaccgaccc cgaccgcgcg
    17221 gcccgcgccc tgctcgaccg cggcgtcgac ctcgccatcg tcaagcaagg acccaagggc
    17281 gttctcgcga tgaccgccga cgagcgggtt gaggcacctc cggtctgggt ggaggtcgtc
    17341 aacgggctcg gagcaggtga cgcgttcggc ggtgccgtct gccacggcct gctcaacggc
    17401 tgggacctgg ccgagacgat ctccttcgcc agcgccgcag gtgcctatgt cgcgggccga
    17461 ctcgagtgtg cgacagcgat gcccgacgag gccgaggtcc gggccctgca ggcccgagcg
    17521 ggtcggtcgt gacgccgccc ctcgacctgg cctcggtgat gcgccggcgg atgctggagc
    17581 cggacggcat acctgcggcg tgggcggcgc ggcgcgccgg tgccgtgccc gccgacggtc
    17641 ggttgctcgt catcgccgcc gatcacccgg cgcgtggcgc cctgggcgtc cgtgacgacc
    17701 gacgcgccat ggcatcccgc tcagatctgt tgcagcgctt ggcgattgcc gtctccaggc
    17761 cgggcgtcga cggagtgctc ggcacccccg acatcctcga agacctgctg ctcgcggggc
    17821 tgctcgacgg cctcctcgtc tacgggtcga tgaaccgagc gggcctgcag ggggcggtgt
    17881 tcgagctcga cgaccggttc acggcatacg acgcggcgac cattgagcgg ctgggtctgg
    17941 acgggggcaa gatgctcgcc cgcgtcgcct acgacgaccc cgcaacggcg ccgacgctgg
    18001 aggcctgcgg ccgggcggtc accgatctcg ccgagcgagg caagatcgcc ctcgtcgagc
    18061 ccttcctctc cagccgcagc gccgacggtt gggtgcgcaa cctcctggac cccgactccg
    18121 tgatcacctc cgtcgcgatc gcctccggcc tcggcgcaac gagcgcccac acctggctca
    18181 aactgccggt cgtgcccgag atggagcggg tcctcgactc gacgaccctg ccgacactcc
    18241 tgctcggcgg cgacccgacc aacgagcccg cgcagaccta cgcgacctgg ggtcgggccc
    18301 tggcctgtcc gcccgcggtg ggcctcgtcg tcggccgcgc cctgctctat ccaccggggg
    18361 gcgacgtcgc ggcagcggtc gatgtcgccg ccgatctcgt ccacggaggc gctcgatgag
    18421 tgattggttg cggcccgccg gctccagcgg cgacgccgag ttcgacgtcc gcatcgcccc
    18481 cggcgacccc gggtggtcgc acaccggcct gtatgccgtg accctcgccg ccggcgagtc
    18541 acgcaccgtc gacaccggtg actgcgagtg gatcgtcctg cccctctccg gtggctcggc
    18601 ggtcgtggag gtcggcgagc gggtctactt cctcaccggc cgggcccacg tcttcgccgg
    18661 cccgaccgac ctggtctacg tcccgcgcga ttcgcggttc accgtgacga gcacggacgg
    18721 gggccggatc gccttcccct tcgccaaggc cagccgcgcc ttcccggtat gccgcgtgga
    18781 cagttcccag gtccccgtcg agatgcgggg gaccgggagt tcctcccgtc aggttcagaa
    18841 cttcgggact cccggtgtgc tggaggctga ttcgatcatt gcctgcgagg tcatcacccc
    18901 ggctggcaac tggtcgtcct atccgcccca caagcacgac gaggaccgtg acggcgtcga
    18961 gtcggcgctt gaggagatct actacgtcga ggttcagcgc gagggtggtg acgggagcgc
    19021 ggcggggatc gcctatctgc gcaactacgg caccgagtcc gcgccgatcg acaccctggc
    19081 cgaggtgcgc tcgggcgacg tcgtcctcat tccgcacggc tggcatggcc cggcgatggc
    19141 gccgcccggc tacgacctct actacctcaa cgtcatggcc ggccccggcg agacgcgggc
    19201 ctggctcatc tgcgacgacc ccgcacacgc ctgggtccgg ggcgcgtggg tcgacgcacc
    19261 ggtggacgcg cggctgccct tcacgcaggt gccctgacgc cgtgcgcatc ggaatcgttg
    19321 gcgtgggccg gatcggctcg ttccacgcac agaccctcgc agccgacccg accgtcaccg
    19381 acctcgtcct gctcgacccg tccgcgacgg cggctgccgc ggtcgcgtcc caactcggtg
    19441 cgacggtcgc ccctgacctc gacgccctcc tgcggcgaga cctcgacgcc ctgctcgtca
    19501 cctcggcgac ccacacccat gccgaggtcc tccgcgccgg cgtgcgggcg ggcctcccga
    19561 ccttctgtga gaagcctgtc gcggccagtc tcgacgagac catcgagctc gcgcggctcg
    19621 aggccgcgtc cggagtcccg atccaggtgg gcttccagcg gcggttcgac gcggcatacg
    19681 ggcgcctgcg tgccgcgatc gcctccggtg agttgggttt cgtccacacg atccgcgccg
    19741 cgacgcacga ccagagcccc ccgccccccg gctacctccc aacatccggt gggttgttcc
    19801 gcgacgcggc gatccacgac tacgacgtca tccgcttcgt cacgggacgt gaggtcgcgc
    19861 aggtgtatgc cgcgggcgcc aacaagggcg agaggttctt caccgaggcg ggagacgtcg
    19921 acaccgcagc cgcgctgctc acgctcgacg acgacaccct cgtcgtcacg tcggtgactc
    19981 gctacaacag ggccgggcac gacatccgga tggaggtcca cggctcgctg ggcgcgctcg
    20041 ccgcaggcct ggacgaccgc tacccgatga cctcggccga gccgggtgcg aggttccccg
    20101 cgggtcccgc gcaccagtcc ttcctcgaac ggttcgccgc ggcctatcgt gcggagatga
    20161 ctgagttcgt ccaggtcgca gccggtgcgg tgccgagccg atgcacggtg gccgacgcgc
    20221 tggaggcgtt ccgggtcgcc gaggcctgtg ggatctcccg ccgcgagggc cgcccggtgg
    20281 cgctggccga gatccccggg gcgtgagtcg tccggcgtcg ggctcctgat ccgtcccctc
    20341 ccatccgagt ctggcgccca gcctcgtctt acgttaatat gttagaacaa atgagaccac
    20401 gacccaagga gcgcgcatga gcaccccccg ccgcatcacc catctcatcg ggcattccga
    20461 atgggacggg accgccgagc ggaccggtga tgtcttcaac ccggcgaccg gcgaggtgac
    20521 cggcaaggtc gacttcgcat cgaaggcgct ggttgacgag gtcgttgcca acgctgccgt
    20581 cgccgctgcc gagtggggtc gggcgtcgct ggccaagcgg acccaggtgc tgttcgcctt
    20641 ccgccagctg ctcaacgaca acaaggagcg cgtcggggcg ctcatcaccg ccgagcacgg
    20701 caaggtgacc tccgacgcga tcggcgaggt gacccgtggt ctggagatcg ccgagttcgc
    20761 ctgcggcatc ccgcacctgc tcaagggtgg cttctccgag aacgtctcga ccaacgtcga
    20821 cgcctattcg atcctgcagc cgctcggggt cgtcggcgtg atctcgccgt tcaacttccc
    20881 ggccatggtg ccgatgtggt tcgtgcccat cgccatcgcc tgcggcaacg ccgtcgtcat
    20941 caagccgtcc gagaaggacc cgtcggcggt caacgcggtg gcggcgctgt ggcgcgaggc
    21001 tggcctgccc gacggcgtca tgagcgtcgt ccatggcgac aaggaggcgg tcgacgcact
    21061 cctggacaac accaccgtca aggcgatctc gttcgtcggc tccacgccca tcgcgcgcta
    21121 tgtctacgag aagggcacgg ccaacggcaa gcgggtccag gccctcggcg gcgccaagaa
    21181 ccacatgctc gtgctgcccg acgccgacct cgacctggcg gccgacgccg cggtcaacgc
    21241 cggcttcggc tcggccggcg agcgctgcat ggcgatctcg gtgctcttgg ccgtcgactc
    21301 gatcgccgac gaactcatcg cgaagatctc cgagcggatg gccaccctca agaccggcga
    21361 cggctcgatg ggctgcgaca tgggtccgat cgtcaccggt gtcgcccgcg accgaatcgt
    21421 cggctacatc gaggccgggg tcggcgccgg ggccgagctg gtcgtcgacg ggcgtgagac
    21481 cgagttccac gggggtgctg acggcttctt cgtcggccca accctgttcg acaaggtcag
    21541 caccgacatg tcgatctaca ccgacgagat cttcgggccg gtcttgtcgg tcgtgcgcgt
    21601 ggagtcctat gacgagggcc tggagctcat caactccagc ctctacggca acggcaccgc
    21661 gatcttcacc aacgacggcg gcgccgcgcg gcggttccag aacgaggtcc aggtgggcat
    21721 gatcggcatc aacgtgccga tccccgtccc gatggcctac tactccttcg gcgggtggaa
    21781 ggcatcgctc ttcggcgaca gccacgccca cggcatggag ggcgtgcact tctacacccg
    21841 caccaaggcg gtcacccagc gctggctcga ccccagccac ggcggcctca acctcggctt
    21901 cccgcagaac aagtagccca cgcgctacgc agcccctccg ggggcggcgc gcggcatacc
    21961 tcaggggtat gccgcgcgcc cgcgcgcgtc tgcggcaggg tgcttccccc tcgccggcgc
    22021 gtcgaatgga tcacaccact agaccgatga ccttggtcta ttgacagcac attaggacag
    22081 atctagggtc aacccagagg aacccccgcg aaaggaactg ccatggccga gtccaaggct
    22141 cgcaacaccg acccccgtta cagcaagctc accatcggcg tctgccccga ccagtggggc
    22201 gtgtggttcc cgcaggacga caagcagatc gattgggacg tcgccctcaa ggagatgcag
    22261 accgcgggct tctcggtcat ggagaccggc ccgttcggct acttccccaa ggacccgacg
    22321 cagttgcgtg acgtcatgga cgagcacggc ttcacggtcg tcgccggcac cggctggggg
    22381 atcctgcaca aggccgaagc ctggaacgac accgagacct tcttccgcgc gatcggcgag
    22441 acgcacgcgg ccgtcggcgc gaagttcgtc gtccacctcc ccccgatgtt ccgcgacgag
    22501 aagtccggcg cctacaccga cgaccgcacc ctgtcgaccg aggcatggaa cctctacatc
    22561 aagaacgccg acaagctcgg gcggatcatg aaggaggact acggcctgca gatggtgctg
    22621 cacccccacg gtgacagcca cattgagacc cccgaggaca tcgaccggat cttccaggcc
    22681 actgaccccg agtacgtcgg cttctgcctc gacaccgggc acatcgtcta tggcggcggc
    22741 gacccgatgg agctgtgccg caagtacccc gagcggatct cctacgttca catcaaggcg
    22801 atggaccagg ccctcgtggc ccaggcccac gacgaggact ggccgttcgt caaggccgtc
    22861 cacgccgggt gctcagtcgc gccgccggcc ggtctgcccg acatgggtca gctcatcgag
    22921 gcgctcgccg acctcgacaa ggagctctat gtcgtctgcg agcaggacat gtacggctgc
    22981 gccaaggact acccgttccc caacgccgtc accacccgcg agtacctcgc gtccctcggc
    23041 ctcggcctca agtagcagca gaagggtgac cctccgatga ctcttcggat cggcatgatc
    23101 ggccccggcg gtatggggca ggcccacatc gaacgcatcc acaccgtcat cgccggcggc
    23161 cgggtcgtcg cggtcagcga cgtcaacgcg gccaccgctc aggcggtcgc cgaccggatc
    23221 ggcgcgacgg catacgccac ctcggctgag ctcatcgcga gcccggacgt cgacgcagtg
    23281 atgatctgca gccatgggcc ggcgcacgag ccggacgtcc tggcctgcat cgcggccggg
    23341 aagccggtct tctgcgagaa gccgctcacc ccgacggccg acgccgcttt gcggatcatg
    23401 gaggccgagc aggctggcgg gcgcaggctc gtcacggtcg gtttcatgcg gcgcttcgac
    23461 gcctcctatc gcgagatgaa ggcgatcctc gacagcggcg acctcggtga ggcgctcatg
    23521 gtgcactgtg cgcaccgcaa ccccaccgtg ccggacttct acacgtggga catggccatc
    23581 aacgacacgg cgatccacga gatcgacacg atgcggtggc tgctcggcga ggagttcgtg
    23641 tcggcgcggg tcgacaagcc gaagaagacg tcgctgcgct tcgagcactt gcaggacccg
    23701 ctggtgctcg tgctggagac ggcctcaggc gtgcgcgtcg acgacgaggt gttcgtcaac
    23761 tgccagtacg gctacgacat ccgctgcgag ctggtcgccg aaaagggcgc cgtctcgctg
    23821 gccgaccaga acgtcgtcac ccggcgtgac tcggtcggcc gcaacaaccg gatctccatg
    23881 aaccacaacg agcggttctg gggcgccttc gtcaccgagg tccaggagtg gatcacggcc
    23941 gcggcgaagg gcgagcactc ggggtcgacc gcgtgggacg ggtatgccgc cgcctgcgtc
    24001 tgcgacgcgg gtgtcaaggc gctcaccgac gacggggtcg tgcccgtgca gatgattgcg
    24061 aagccggact tctacgcctg acgcagggaa gcggggcgtc agtaggtgag gcccgaccga
    24121 gacccgtctc ggtcgggcct cgactgttgt
//
LOCUS       DAORPW010000019        23948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_337_length_23948_cov_845.786130, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000019 DAORPW010000000
VERSION     DAORPW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 23948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23948
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_337_length_23948_cov_845.786130"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..939
                     /locus_tag="PLB86_03085"
     CDS             178..939
                     /locus_tag="PLB86_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRC64149.1"
                     /translation="MGLSDVLYGVYERRLAHDLDRSNVPRHVGVMLDGNRRWAKANGF
                     DTVGGHQAGADKIQDLLAWCEETGVELVTLWLLSTDNLDRDSSELEPLLGIIEEAVND
                     LAAAQRWRLHPIGALDLLPEATATALLAAETATAGVTGLTVNVAVGYGGRREIADAVR
                     QLILEQAATGIPIEEIADKVSVEAIAQHLYTRGQPDPDLVIRTSGEQRLGGFLLWQSA
                     KSEFYFCEAYWPDFRRVDFLRALRAYANRERRFGR"
     assembly_gap    1087..1186
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1187..3186)
                     /locus_tag="PLB86_03090"
     CDS             complement(<1187..3186)
                     /locus_tag="PLB86_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRC64150.1"
                     /translation="MSKSGDGDNASRALPESPVEVDQEQRDQEAAKQAARHLKRLRKR
                     ELAAAALVQAGGDPDPEDFLARRPSIEYHTSTTGPSLMTKALLAGTPGLRTLQHYRRH
                     WLRSDIFAGVAVVAYMIPQCMAYSAIVGVPPEVGLSTALGALIVYAVMGQSRMLSVGP
                     ESTVALIAGVAIAPFANGDPVKATTLGAALSLIVAGWCFVARMLRLGIVAELLSQPLL
                     VGYLAGAAVLMVVGQLGKMTGTKVHGESIVEQVQSFLGVASGTHLFTLWVGIGTFVFL
                     MLIHWVRHRWPATLIAVAVATVVCTVFQLKNQGVAVLGTVPTGLPLPSIPAVSWSEIE
                     ALLAAGVGVAIVAYGDNTLVARGFPAPIDPDEDRAVNQLDPQQELIALGGCHVAVGLL
                     GGFPVSSSGSRTALAVASGARTQMYSVVAAFMLIIVLFVAGPLIANLPQAALAAVVFY
                     AASKLINVKEIKRLARFRKQELLLAMAATVGTVFFGVVTGIGIAIALSVLEMTQRLAR
                     PHDGVLGRVPGLPGMHDVADYPTAQTLPGLIVYRYDAPLFFANVGDLRRRATMVVDQE
                     NKAFPQSPARWFILNVEANVEVDLTAADGLAELQRDLAERGVRLGLARVKQDLLLPLQ
                     RAGLMELIGTDMLFPTLPVAEKAYLIWSAQNPFTPPEPLETES"
     gene            3458..4783
                     /locus_tag="PLB86_03095"
     CDS             3458..4783
                     /locus_tag="PLB86_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRC64151.1"
                     /translation="MAASRTSANGAAGRKTYVLDTSVLLSDPRAITRFAEHEVVLPVV
                     VVTELEAKRHHPELGYFARTALRMLDDLRVAHGRLDCALPIGDLGGTVRVELNHSNPD
                     VLPAGLRLGDNDTRILAVAKNLAADGNDVTVVSKDLPMRVKASAVGLDAQEYRAELAV
                     DSGWTGMADLDMTVEDMAELYEHGRVEHAAAAELPCHTGLVLHSPRGSGLGRVGADKQ
                     VRLVRGDRDAFGLHGRSAEQRIALDLLLDPDVGIISLGGRAGTGKSALALCAGLEAVL
                     ERRQHRKVVVFRPLYAVGGQELGYLPGTEAEKMGPWGQAVFDTLGALLSTEVVEEILD
                     RGMLEVLPLTHIRGRSLHDAFVIVDEAQSLERNVLLTVLSRVGQNSRVVLTHDVAQRD
                     NLRVGRHDGVAAVIETLKGHPLFAHVTLTRSERSPIAALVTDLLERLDL"
     gene            4941..5738
                     /locus_tag="PLB86_03100"
     CDS             4941..5738
                     /locus_tag="PLB86_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64152.1"
                     /translation="MPRVERMIVAERYVGRHRRAELALAAATAGSAPKGLAFRPLLHA
                     GASVALATAAVGGYLTAGGAADTNGVTLADPAFASANVAASLSRTLADAPVALTAFDA
                     TAADAEIAQANRERVSRVVSVTTANEIAAQAAAAEAARLDVERQAAAERATRDAQRAS
                     ILANAQRDPKAVAAVLVADRGWSTAQFGCLVKLWTKESNWRWNADNPTSSAYGIPQSL
                     PGSKMASAGADWRTNPVTQIKWGLGYIASRYGSPCSAWSHSVNHNWY"
     gene            complement(5777..6748)
                     /gene="coaA"
                     /locus_tag="PLB86_03105"
     CDS             complement(5777..6748)
                     /gene="coaA"
                     /locus_tag="PLB86_03105"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HRC64153.1"
                     /translation="MWRVTQRRESNGSFPTPFVELDRSAWSRLAKNHPLEITADDLTR
                     LRGLDHPLNLGEVQEVYLPLSRLLTFYCEAVEQLHGVTSDFLMERRQRTPFVIGVAGS
                     VAVGKSTTSRILRELLSRWPSTPNVELVTTDGFLLPNAELRRRGLMHRKGFPESYDRR
                     ALLRFLAEVKGGRPEVSVPVYSHLSYDIVPGERIVVRQPDVLIVEGLNVLGAPPVRPM
                     GGTGMAVSDYFDFSVYVDARVSHIERWYVDRFLTLRATAFANPNAYFHRYAALSDEEA
                     VATALTIWETINGPNLVENILPTRQRATLVIVKGEDHSVQKVRLRKL"
     gene            6805..8664
                     /gene="glmS"
                     /locus_tag="PLB86_03110"
     CDS             6805..8664
                     /gene="glmS"
                     /locus_tag="PLB86_03110"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRC64154.1"
                     /translation="MCGIVGYVGQASDGTALDVVMEGLARLEYRGYDSAGVAVIDGDH
                     VAFRKRSGKLVNLQDAIAAEPLPQTATAIGHTRWATHGGPTDDNAHPHLGGSHGRLAL
                     IHNGIIENFHALKTELLEQGVAFASETDTEVAAHLVSAAYDRTHDLTAAMLEAVARLD
                     GAFTLLAIHADQPGVVVGARRNSPLVVGLGTGANYLGSDVAAFIGHTREALELGQDQI
                     VTITPDSVTVLGFDGTPAEGRRYHVDWDAAAAEKGGYSSFMAKEIDEQPHAVADTLLG
                     RTKENGELVLDELRVSEDALRAVDKIVIVACGTAAYAGMVAKYAIEHWTRIPCEVELA
                     HEFRYRDPVVDGNTLVVSVSQSGETMDTLMAVKHARELGALTVSICNTHGSTIPRESD
                     AVIYTHAGPEIAVASTKAFLAQITACYLLGLYLAQLRGGAFADEARAVMRDLREMPDK
                     IQTVLDRLGRVREIARFMADTRSVLFLGRHVGFPVALEGALKLKEIAYIHAEGFAAGE
                     LKHGPIALIEPGQPVFVIVPGPDAPHDLHAKVVSNIQEIRARGARTLVVAQDGDEAVV
                     PFADEIIRVPATAPLLQPLLTVVPLQVFACELATAKGLDVDQPRNLAKSVTVE"
     gene            8669..9025
                     /locus_tag="PLB86_03115"
     CDS             8669..9025
                     /locus_tag="PLB86_03115"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRC64155.1"
                     /translation="MILGVGIDVVDIDRFGASLERTPALRQRLFTESERGLPLRSLAA
                     RFAAKEALAKALGSPVGLSWSDATVLRGEDGRPELDVRGSVLARTKELGIQTLHVSLS
                     HDAGIASAVVVAEGEP"
     gene            9022..10488
                     /locus_tag="PLB86_03120"
     CDS             9022..10488
                     /locus_tag="PLB86_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRC64156.1"
                     /translation="MIQAYAVADVRAVEAALMEHLPEGELMARAASGLAEVVRARLAE
                     RGGHRVVALVGGGNNGADALYAAALLAQAGFAVGAVCTAYGSVHQGGRDAALAAGVVV
                     TGGDGPEWEALLAEADIVVDGITGIGGRPGLGVEAQGWVGAIPETAYVVAVDLPSGLD
                     PAGVEGTAECVFADETVTFGVAKPCHLLPATEAAVGRLTVVDIGLDFAGCTPVVERLL
                     GADVAAAWPVPTAASDKYSRGVVGVVAGSAAYPGAGVLCVLGALGTGPGMVRYLGPTS
                     VAALVHGHAPEAVTATGRVQAWVLGSGVDPEDTDDEDQLRWIRQALDSDLPCVVDAGA
                     LALLGPRSAPTLITPHAGELARLLTRLAGAGEAEVTREDVLGRPIDHAQAAADALGAV
                     VLLKGATTLVVSPTAAGRAIRSQADAPPWLATAGAGDVLAGILGTLVAAGLDLVNAAS
                     VGAFVHGVAADRANPGGPVRALAVARKVPGVVAAVLTA"
     gene            10534..11703
                     /gene="alr"
                     /locus_tag="PLB86_03125"
     CDS             10534..11703
                     /gene="alr"
                     /locus_tag="PLB86_03125"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRC64157.1"
                     /translation="MTSPLPPARPGSQATAYVDLGAVEGNVRALRGHAGPAALMAVVK
                     ADAYGHGLVPVARAAIRGGATWLGTARLSEAITLRDNGIPGRLMAWQAVPGADFAACL
                     EREIDLGVSAPWALDEIVAAARHTGITARVHLKVDTGLGRAGAYAADFTALLDAAVAA
                     AREGTVRVVGVWSHLACADEPGHPSIRAQQLVFEAAVAEAARAGCELEVRHLANSAAT
                     VTDPSLHYDLVRTGIAIYGLSPVPALGDSAAYGLTPAMTLVARLALVKDVPAGQGVSY
                     GHLYTTTEATRLGLVPLGYADGIPRNASNVGPLAVGGVRHRIAGRVCMDQVVVDLGAG
                     SNARAGDDVVIFGTGGAGEPTAQEWAVSTGTISYEIVTRLGPRVEREYVGVEEVG"
     gene            11703..12899
                     /locus_tag="PLB86_03130"
     CDS             11703..12899
                     /locus_tag="PLB86_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC64158.1"
                     /translation="MARGRPNLVGLGVSLGAAGAAGALGAAGLIADRIRRREDAQLPE
                     YSSLIEAPSSELTVIADDGVTLYVAIDEATGPTKTAAGHPKPTVVLSHGYCLTSECWV
                     LQRRALRRAGYRVVTWDQRGHGRSGMGPKVGYHIDQLGRDLYAVLQAAAPTGDLALIG
                     HSMGGMTIMALAESHPDVIRDRTIAFGAIATSPGSLPLANGGFVAATGKQVLERLGPS
                     VFTGLSKRPELLGTLLKANRDLEEFLVERYSFASPVPRSVVRLTARMLMGTDLAVMSD
                     FVPTFDAYNKIPALAHFAHIETLVFNGTQDVLTPPAHSELIVRGIPGSEHVLIRDAGH
                     VIMLEHPDLLNDQILALLDRAELGRAKHLDPAEKPHVTLHVTPVGKARRVRDGLRNSR
                     KREHVS"
     gene            12889..13407
                     /gene="tsaE"
                     /locus_tag="PLB86_03135"
     CDS             12889..13407
                     /gene="tsaE"
                     /locus_tag="PLB86_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRC64159.1"
                     /translation="MSPSSGPLTIRLPDADAARAFGLALATVLRAGDVLVLTGDLGAG
                     KTTLTQGLGEGLGVRGPITSPTFVIARVHPSLVGGPALVHVDAYRLGGAVELDDLDLD
                     ADLDASVVVVEWGHGVAEQLSADRLEVVLQVVGPGETRQATVWATGERWADPTARAAL
                     AALASGAPLADD"
     gene            13417..14073
                     /gene="tsaB"
                     /locus_tag="PLB86_03140"
     CDS             13417..14073
                     /gene="tsaB"
                     /locus_tag="PLB86_03140"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRC64160.1"
                     /translation="MLLLALDTSTSAITVALHDGDRVLAQATTLDARAHGERLAPGIQ
                     QVLAQAGATPTELTHVVCGLGPGPFTGLRVGIVTARVLAFTTGAALHGMCSLDALAAD
                     GPTGEILVATDARRKEVYWARYAVSPGSVRALTPAAVDRPADLPEQVRALPTLGRGPL
                     LYPELFPAPVGILDVSAAALAGLAVSRLAAGAALSDHEPLYLRRPDAAPHIVTKSALG
                     "
     gene            14070..14531
                     /gene="rimI"
                     /locus_tag="PLB86_03145"
     CDS             14070..14531
                     /gene="rimI"
                     /locus_tag="PLB86_03145"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRC64161.1"
                     /translation="MSAREVTMRELRWSDIPALAQAERELFADDAWTEPTWWAELAGR
                     PRRDYVVAAASDGTIAGYAGVDCAGDTADVMTIAVDTAYRGTGLGDRLLGDLLGRASA
                     AGAQSVLLEVRADNAPALGLYGRHGFEQVSVRRRYYQPGDVDALVLRRRLP"
     gene            14528..15574
                     /gene="tsaD"
                     /locus_tag="PLB86_03150"
     CDS             14528..15574
                     /gene="tsaD"
                     /locus_tag="PLB86_03150"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRC64162.1"
                     /translation="MNDEPLVLGIETSCDETGIGIVRGSTLLVDAVASSVDEHARFGG
                     VVPEVASRAHLEAMVPTLERACRDAGVALRDIDAVAVTAGPGLAGALLVGVAAAKALA
                     VGLGKPIYGVNHLAAHVAVDVLEHGPLPEPTMALLVSGGHSSLLLVPDVTGDVRPLGA
                     TIDDAAGEAFDKVARVLGLPFPGGPHIDRVAREGDRVAIDFPRGLTTGRDLDRYRFDF
                     SFSGLKTAVSRWVQVRQAAGESVPVADVAASFQEAVVDVLTRKAVAACRDQGVDDLLI
                     GGGVAANSRLRALAQERCDRAGIRLRVPRPGLCTDNGAMVAALGAQFVARGRTPSLLD
                     LPADSSMPVTSVQA"
     gene            complement(15599..16612)
                     /locus_tag="PLB86_03155"
     CDS             complement(15599..16612)
                     /locus_tag="PLB86_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRC64163.1"
                     /translation="MTPAQRWGQLIMVGIQPAQAPGGIDATIRASHVGSVIYLGGWSG
                     AGTVRATSDHLQQVATGPADLFIAADQEGGAVQQLKGKGFTVLPAALDQGKLPPSELT
                     ALGTTLAAELRSAGVNVNLAPVADTVPPSMVQTNAPIGKYRRELGNDPAVVAAAVPVL
                     VTAVQAGQVVATIKHFPGLGRITGNTDVTAVGITDSQATVDDPYLEPFRAGIAAGTGM
                     VMVSSARYPKLDDANPAMFSRAIVTDLLRTSLGFDGVIVTDDVGAAKAVAAIPVPDRA
                     VRFLAAGGDLVLTASAAQVPSMIAAIKARAASDPAFAAEIEQSVRRVLALKARFGLLR
                     CGG"
     assembly_gap    16789..16888
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17043..17795)
                     /locus_tag="PLB86_03160"
     CDS             complement(17043..17795)
                     /locus_tag="PLB86_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC64164.1"
                     /translation="MTEPAIPRPTPGRADGIRARNRAGIEAELRAAARRQLAEVGPAA
                     LSLRAVARDMGMVPSALFRYVSGRDELLTLLIVEAYTSLADAVDEAHDAVDPADLHGR
                     WAALATSMRAWALANPHEWALLFGSPVPTYQAPSEQTTDPGTRVSRLLLAIGRDAALR
                     GVAGSAPFGPATRAADLAAAATANLISEPDVTSSGMSAVTLANGLATWAMLVGAVSSE
                     VFEQFGPQTIANHEAFFTYQVAIGESLLFGRG"
     gene            17953..18915
                     /locus_tag="PLB86_03165"
     CDS             17953..18915
                     /locus_tag="PLB86_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HRC64165.1"
                     /translation="MSRHVVVGAGGIGRATSLALAEAGHEVVLASKSGVGRELPGVKR
                     VAVDATDRRALTALAEGAASLVNAVNPPTYTHWDRDWPPMAAAFLTAAQASGAGLVTI
                     GNLYGYGPVDGPIHPGLPLAATGVKSRVRAQMWADALAAHQAGRVKATEVRASDYFGP
                     DIQPQASVLQRFVFGPAAAGKAVRPFDGSPDAPHSWSYVPDIGAVAAALATSDLGWGR
                     AWHVPSGMPRSLAQAAADAAAFAGHPAPAIRPVAPWVRGLLGTFVPVIRELDETAYQR
                     ERAFVIEAAETESTFGVTATPWQDALAATLTAMGVVPAAASSRV"
     gene            18967..19785
                     /locus_tag="PLB86_03170"
     CDS             18967..19785
                     /locus_tag="PLB86_03170"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRC64166.1"
                     /translation="MGRVLVLGSLNVDVVARVERLPQPGETVSAVASIQRLAGGKGGN
                     QAVAAAAAGARVVMVGAVGDDEPGRAYTVRLAGRGIFPRLAIAFGVPTGTAYVWVDEN
                     GENSIVVAPGANALARAEVADLTPDDVLLCQLEVPVDVVADAVRTAARLGARVVLNAA
                     PYAALPDDVLAVADPLVVNEHEAGLLADSGAAVGSLLVTFGAAGAQWDGERVDGIPVP
                     ADEVLDSTGAGDAFCGALAARLAEGDDRRSALAWAVRAGADAVRREGAQPDPGL"
     gene            complement(19782..21026)
                     /locus_tag="PLB86_03175"
     CDS             complement(19782..21026)
                     /locus_tag="PLB86_03175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC64167.1"
                     /translation="MDPALLARLASGPGRALLDSLEGYAADSALALSTRLRAAGHEPE
                     LVTAVLAQARLRGLAVEKFGAAAGGMLFTADGLEQATRPELASRHASRFVAAGVDHVV
                     DLGCGIGSDARAFAASGLSVEAVEVDPVTAAVARANLAEWPNAHVSEGSAETVDLAKA
                     RGAREVSGRHTGVWVDPGRRVSGVRDSAGRARRVFSLEAISPPWPQVLAWAAQVPATG
                     AKLSPAFPHAALPSGTEAQWTSWRGEVLECAIWWGPLAQVPGRTAAVCRPGASPAIVT
                     EADVREAPVIGGLDEVGPWFYEADRAVVRAGLSGALPGRELAAGVGYATGSEPIDLPW
                     ARQYAVVEAMPLRVKAVRAWLRARGIGRVTVKKRGVSVDPEALRRELTTRAEGHATLV
                     VTSVAGHPVAIVVDAAVSATGV"
     gene            21084..22286
                     /locus_tag="PLB86_03180"
     CDS             21084..22286
                     /locus_tag="PLB86_03180"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC64168.1"
                     /translation="MTAPLEGLLVADFSRVLAGPFATMYLADLGATVVKVERPGSGDD
                     TRAWGPPWTEHTSSYFESVNRSKLGLTLDLADPADQAMARELARRADILVENFRPGTL
                     DRMGLGYEQVRDGDPATGRPANPGIVYASITGYGSGAGASIPGYDFVVQAVGGLMSIT
                     GEPDAPAQKVGVALVDVLTGKDAVIGILAGIAARARSGEGVRVEVDLLSSLLGALVNQ
                     ASGYLATGTPPQRMGNRHPSIAPYETLACADGQLAVACGNDGQFRRLAAAVGLAHLAD
                     DAAYATNSARVAHRDALAIALEGRLRAQPVAHWDGVLRGVGVPAGPVGDIGSAFALAR
                     DLGLEPTYAAHPGHVAQVRNPVRLSGHDPGPGMPPPRLGEHDAVVRGWLSGGDPSLAR
                     LALDLTEC"
     gene            22421..22714
                     /gene="groES"
                     /locus_tag="PLB86_03185"
     CDS             22421..22714
                     /gene="groES"
                     /locus_tag="PLB86_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRC64169.1"
                     /translation="MSVSIQPLEDRILVKSLEAETTTASGLVIPDTAKEKPQEGEVLA
                     VGPGRMTDKGVRVAMDIKVGDRVIYSKYGGTEIKHGGQEFLLLSARDVLAIVG"
     gene            22727..>23948
                     /gene="groL"
                     /locus_tag="PLB86_03190"
     CDS             22727..>23948
                     /gene="groL"
                     /locus_tag="PLB86_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144865.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRC64170.1"
                     /translation="MAKTLEFDDAARKSLERGVDALANAVKVTLGPKGRNVVIDKKWG
                     APTITNDGVTIAREIELEDPYENLGAQLAKEVATKTNDIAGDGTTTATVLAQAMVKEG
                     LRNVAAGAAPSALKRGMDKAVEALNDRLLANARELEGTEEIGQVAALSAQDEAIGATI
                     AEAFDKVGKDGVISVEESSSTATELEFTEGMQFDKGYISAYFVTDAERMEAVLEDAYI
                     LINQGKISSIQDLLPVLEKVVQTGKPLVVVAEDVDGEALSTLVVNKIRGTFTVVAVKA
                     PGFGDRRKAMLQDLAILTGGQVISEEVGLKLDQAGLDVLGQARRVVVTKDTTTVIDGQ
                     GGKDDVDGRVAQIKKEIERTDSDWDREKLQERLAKLAGGVCVIKVGAHTEVELKEKKH
                     RIEDAISATRAAIEE"
BASE COUNT         3479 a         8326 c         8468 g         3475 t
ORIGIN      
        1 tccgggttaa acgggggtgc gggggcgtaa agatgaccgt gcgagcgcta tctcctgggt
       61 cagggggttc gcgggggttt cggggtgcgg gcgggggatg ggagcgacgc ggcatacggg
      121 agtagcgtgt cgggaacagc cgacaacggc gaggttcgcg aacggaaggc gagtcgggtg
      181 ggcctgagcg acgtgctcta cggcgtctac gagcgtcggc tggcgcacga tctcgaccgg
      241 tcgaacgtgc cgcggcacgt cggcgtcatg ctcgacggca accggcgctg ggccaaggcc
      301 aacggcttcg acacggtggg cggacatcag gccggggccg acaagatcca ggacctgctc
      361 gcctggtgcg aggagaccgg cgtcgagttg gtcaccctgt ggctgctctc gaccgacaac
      421 ctcgaccgcg actccagcga gttggagccg ctcctgggga tcatcgagga ggccgtcaac
      481 gacctcgccg cggcccaacg ctggcgcctg cacccgatcg gtgcgctcga cctgttgccg
      541 gaggcgacgg cgaccgcact gctggcggcc gagaccgcga cggcgggcgt cactggtctc
      601 accgttaacg tcgccgtggg ttacggcggt cgacgcgaga tcgccgacgc cgtgcggcaa
      661 ctcatccttg agcaggccgc gaccggcata cccatcgagg agatcgccga caaggtgagc
      721 gtcgaggcga tcgcccaaca cctctacacg cgcggacaac ccgatcccga cctggtgatc
      781 cgcacctccg gcgagcagcg cctcgggggc ttcctgctct ggcagagcgc caagagcgag
      841 ttctacttct gcgaggccta ctggccggac ttccggcggg tcgacttcct gcgcgcgctg
      901 cgggcctacg ccaaccgcga gcgccgcttc ggccgctgag acctcacccg agcgcgcgcg
      961 gtcaggagcc cgcgtgggtg cgagccccgg tgagtatgcc gttcaccgcg ccattgctcg
     1021 ggttgccgtc ggtggcgttg cccttcttgc ccttcttgcc cttggcggtg ccgttgctgg
     1081 cggcatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngact cggtctccag
     1201 gggctccggc ggcgtgaacg ggttctgtgc cgaccagatg aggtaggcct tctcggcaac
     1261 agggagcgtc gggaagagca tgtcggtgcc gatgagctcc atgagcccgg cccgctgcag
     1321 ggggagcagg aggtcctgct tgacgcgcgc caagcccaaa cgcacccccc gctcggcgag
     1381 gtcacgctgc aactcggcca gcccgtccgc ggcggtgagg tcgacttcga cgttggcctc
     1441 gacgttgagg atgaaccagc gcgcggggct ctgcgggaag gccttgttct cctggtcgac
     1501 gaccatggtg gcccgacggc gcaggtcgcc gacgttggcg aagaacaaag gggcgtcgta
     1561 gcggtagacg atcaggcccg ggagcgtttg agcggtcggg tagtcggcga cgtcgtgcat
     1621 gccgggcagg cccgggacgc gcccgagcac accgtcgtgg gggcgggcca gacgctgggt
     1681 catctccaga acgctgaggg cgatagcgat gccgatcccc gtgacgacgc cgaagaagac
     1741 ggtcccgacg gtggcagcca tcgccagcag caactcctgc ttgcggaagc gagccagccg
     1801 cttgatctcc ttgacgttga tcagcttgct ggcggcatag aagaccacgg cggccagggc
     1861 agcctgcgga aggttggcga tcaacggtcc ggcgacgaac aggacgatga tcagcatgaa
     1921 cgccgcgacc accgaataca tctgggtgcg tgcgccggac gccaccgcga gcgctgtgcg
     1981 ggagcccgag gacgagacgg ggaaaccacc gagcaggccc acggcgacgt ggcagccgcc
     2041 cagcgcgatc aactcttgct ggggatcgag ttggttgacg gcgcggtcct cgtcggggtc
     2101 gatgggggcg gggaacccgc gggccacgag ggtgttgtcg ccgtaggcga cgatcgccac
     2161 tccgacaccg gctgccaaca gagcctcgat ctctgaccag gacaccgccg ggatggacgg
     2221 gagaggcaga ccggtgggaa ccgtgcccag caccgcgacg ccctggttct tgagctggaa
     2281 gacggtgcac acgacggtcg ctacagccac cgcgatgagc gtggctggcc agcgatgcct
     2341 cacccagtgg atgagcatga ggaagacgaa cgtcccgatg ccgacccaga gggtgaacag
     2401 gtgcgttcca ctcgcaacgc cgaggaacga ttggacctgc tcaacgatgc tctcgccgtg
     2461 gaccttcgtg ccggtcatct tgccgagttg gccgacgacc atgaggacgg ccgcgccggc
     2521 gagatagccc accaacagcg gctgggagag caactcggcg acgatgccca ggcgcagcat
     2581 ccgagcgacg aaacaccatc ccgcgacgat gagcgaaagc gcggctccca aggtggtggc
     2641 cttcaccggg tcaccgttgg cgaacggcgc gattgccacg ccggcgatga gggcgacggt
     2701 cgactccggg cccacggaaa gcatccgcga ctggcccatc acggcataca cgatgagggc
     2761 gccgagcgcg gtcgagaggc cgacctccgg gggcactccc acgatcgcgg agtaggccat
     2821 gcactgggga atcatgtagg cgacgacggc cacgcccgcg aagatgtccg agcgcaacca
     2881 gtgccggcga tagtgctgca gggtccgcag gccgggggtc ccggcgagca gggccttggt
     2941 catcaggctc gggccggttg tcgacgtgtg gtactcgatg gagggacggc gggcgaggaa
     3001 gtcctcggga tccgggtcgc cccccgcctg caccagggcc gcagccgcca actcgcgctt
     3061 gcgcagtcgc ttgaggtgtc gggctgcctg cttggcggct tcctgatcgc gttgctcctg
     3121 gtccacctcg acgggcgatt ccggcagcgc tctgctcgcg ttgtcaccat ctccagattt
     3181 ggacatggga tcacccccac acgttgtcag atggccggct ggtgaacatg ggatgaccgg
     3241 ccggaagagt cgcgcccaat ctaggggaac caacgaccgc gcgcaccccc ttgcccagcg
     3301 cgacggcata ccggctgggt atgccgcgtc gccgccgcaa cggcgcaccc cgcgactcgc
     3361 ttcgctcagg ggtgcgcgac gcgccgggcc ggatcccgcc cggcggcgcg gtgggcgctt
     3421 accgtcggag cacgtgcaga tcgccaagga ggggtgcgtg gctgcttctc ggacgtccgc
     3481 gaacggtgct gcgggtcgaa agacctacgt tctggacacg tcggtcctgt tgtccgatcc
     3541 gcgagcgata acgaggttcg ccgaacacga ggtggtgctc ccggtcgtcg tcgtcaccga
     3601 gctggaggcc aaacgccacc acccggagtt gggctacttc gcccggacgg cgctgcggat
     3661 gctcgatgac ctgcgggtgg cgcacggtcg gttggattgc gccctgccga tcggcgacct
     3721 cggcggcacg gtgcgcgtcg agctcaacca ctcgaacccc gacgtcctgc ccgcgggact
     3781 tcggttgggg gacaacgaca cccggatcct cgcggtggcc aagaacctcg ccgccgacgg
     3841 caacgacgtc accgtcgtca gcaaggacct gccgatgcgg gtgaaggcct ccgcggtggg
     3901 cctggacgcg caggagtatc gggctgagct cgccgtcgac tccggctgga cgggcatggc
     3961 cgacctcgac atgaccgtcg aagacatggc tgagctctat gagcacggtc gggtcgagca
     4021 cgccgccgca gccgagttgc cctgccacac cgggctggtg ctgcactcgc cgcgcggcag
     4081 tggcctgggt cgggtcggcg ccgacaagca ggtgcgcctg gtgcgtgggg accgcgacgc
     4141 cttcgggctg cacggccggt cggccgagca acgcatcgcc ctggacctct tgctcgaccc
     4201 cgatgtcggg atcatctctc tcggcggccg cgcggggacc ggcaagtccg ccctcgccct
     4261 gtgcgcgggg ctcgaggccg tcctcgaacg gcgccagcac cgcaaggtgg tcgtcttccg
     4321 gccgttgtat gccgtcggcg gccaggagtt gggctacctg ccgggcaccg aggccgagaa
     4381 gatgggtccg tgggggcagg ccgtcttcga caccctcggc gcgttgctct ccacggaagt
     4441 ggtggaggag atcctcgacc gggggatgct ggaagtcctg ccgctcaccc acatccgcgg
     4501 ccgctcgctg cacgacgcgt tcgtcatcgt cgacgaggcc cagtcgttgg agcgcaacgt
     4561 cctgctgacg gtgctctccc gggtcgggca gaactcccgg gtcgtcctca cgcacgacgt
     4621 cgcccagcgc gacaacctgc gggtcgggcg ccatgacggg gtggcggccg tcatcgagac
     4681 cctcaagggc cacccgctct tcgcccatgt gacgttgacc cgcagcgagc gcagcccgat
     4741 cgctgctctg gtgaccgacc tgctcgaacg cctggacctc tgaccctgcc gctctcgatg
     4801 ccggggtgga gatttgtccg ttcggctgat ctctggcact ctatgacgaa gatctcgact
     4861 tggtaacgcc gtgacggaat ccgtcgtgcg ttccgtctag agatgtggga gagcacggcc
     4921 agatgcgaca tcgaccaacg atgccgcgcg tcgaaaggat gatcgtggcg gagcgttatg
     4981 tcggtcggca ccgccgagcc gaactcgcgc tcgctgcggc gaccgcggga tcggcaccca
     5041 agggcctcgc gttccggccg ctgctgcacg ccggggcctc tgtcgcgttg gccacggccg
     5101 ccgtcggcgg ttacctcacg gccggtggcg cagcggacac caacggcgtc accctcgccg
     5161 acccggcttt cgcatcagcg aatgtcgcgg cgtccctgag ccggaccctc gccgatgccc
     5221 ccgtcgccct cacggcgttc gacgcgactg ctgccgacgc cgagatcgcc caggccaacc
     5281 gcgagcgggt cagccgggtc gtgagcgtga cgaccgccaa cgagatcgcc gcgcaagcgg
     5341 ccgccgctga ggccgcccga ctggacgtcg aacggcaggc cgccgccgag cgggccacac
     5401 gtgacgccca acgggccagc atcctggcca acgcccagcg tgaccccaag gcggtcgccg
     5461 ccgtgctggt ggccgaccgt ggctggagca ccgcgcagtt cggctgcttg gtcaagttgt
     5521 ggaccaagga gagcaactgg cgctggaacg ccgacaaccc gacgtcgagc gcctacggca
     5581 tcccgcagtc tctgccgggg tcgaagatgg cgtccgcggg tgccgactgg cgcaccaacc
     5641 cggtcaccca gatcaagtgg gggctgggtt acatcgccag ccgctacggc tcgccgtgca
     5701 gcgcgtggag ccactcggtc aaccacaact ggtactgacc gccgggtcga gcgcagccag
     5761 atcaggcact gaccgatcag agtttgcgca gtcggacctt ctgcaccgag tgatcctcgc
     5821 ccttgacgat gaccagcgtg gcccgctggc gggtcgggag gatgttctcg acgaggtttg
     5881 ggccgttgat cgtctcccag atcgtcagcg ccgtcgccac ggcctcctca tcggacaacg
     5941 cggcataccg gtggaagtag gcgttcgggt tggcgaaggc ggtcgcgcgc agggtgagga
     6001 accggtcgac gtaccagcgt tcaatgtgtg acacccgagc gtcgacatag accgagaagt
     6061 cgaagtagtc gctcactgcc atcccggtgc cgcccatcgg gcgaacgggc ggcgcgccga
     6121 ggacgttgag cccctcgacg atgagcacgt cgggctgacg cacgacgatc cgctcacccg
     6181 ggacgatgtc gtaggacagg tgtgagtaga ccggcaccga cacctcgggc cgtccgccct
     6241 tgacctctgc gaggaaccgc aacaacgctc ggcggtcgta agactcggga aaacccttgc
     6301 ggtgcatgag tcctcgccga cgaagctcgg cgttcggcag gaggaaaccg tcggtcgtga
     6361 cgagctcgac gttgggggtg gacggccagc gcgacagcag ttcccgcagg atgcgcgagg
     6421 tcgttgattt cccgacggcc accgatccgg caacgccgat gacgaagggc gtgcgctggc
     6481 ggcgctccat gaggaagtcg ctggtcacgc cgtgcaactg ctcgacggcc tcgcaataga
     6541 aggtcaggag ccgtgagagc ggcaggtaga cctcttggac ctcgcccagg ttcagcgggt
     6601 ggtcgaggcc gcgcagccgg gtgaggtcgt ccgccgtgat ctcgagcggg tggttcttgg
     6661 ccagccgaga ccacgccgac cggtccagtt ccacgaaggg tgtgggaaaa ctcccgttgc
     6721 tctcacggcg ctgggtcacg cgccacattc ttgcgtatgc cgggcgggac agggccgcag
     6781 gtggggggaa ctacgctggt ggccatgtgt ggaatcgtgg gttacgttgg tcaagcgtcg
     6841 gacgggacgg ccttggacgt cgtcatggag gggttggccc ggttggaata ccggggctac
     6901 gactccgccg gtgtggccgt catcgacgga gaccacgtcg ccttccgcaa gcgttcgggg
     6961 aagctcgtca accttcagga cgccatcgcc gcagagccgt tgccgcagac ggcgacggcc
     7021 atcggccaca cccgttgggc cacccacggt ggacccaccg acgacaacgc ccacccccac
     7081 ctcggcgggt cccacggccg gctggcgctc atccacaacg ggatcatcga gaacttccac
     7141 gcgctcaaga ccgagttgct cgagcagggc gttgcgttcg cctccgagac cgacactgag
     7201 gttgctgccc atctggtgtc ggcggcctac gaccgcacgc acgacctcac ggccgccatg
     7261 ctcgaggcgg tcgcccgcct ggacggcgcc ttcaccctgt tggcgattca cgccgaccag
     7321 cccggtgtcg tggtgggtgc ccggcgcaac agccccctgg tggtcggcct cgggacggga
     7381 gccaactacc tcggctccga cgtcgcggcc ttcatcgggc acacccgtga ggcgcttgag
     7441 ctcggccagg accagatcgt gacgatcacc cccgactcgg tgacggtcct cgggttcgac
     7501 ggaaccccgg ccgagggtcg ccgctatcac gtcgactggg acgccgccgc agccgagaag
     7561 ggcggttact cgtcgttcat ggccaaggag atcgacgagc agccgcacgc cgtcgccgac
     7621 accctgttgg gccggaccaa ggagaacggg gagttggtcc tcgacgaact gcgcgtctcc
     7681 gaggacgccc tgcgcgccgt cgacaagatc gtcatcgtcg cgtgtggcac ggccgcctac
     7741 gcagggatgg tcgcgaaata cgccatcgag cactggaccc ggatcccgtg cgaggtcgag
     7801 ctggcccacg agttccgcta tcgcgacccg gtcgtggacg gcaacacgct cgtcgtgtcg
     7861 gtgagccagt ccggcgagac gatggacacc ctcatggccg tcaaacacgc ccgcgagctg
     7921 ggggccctca ccgtctcgat ctgcaacacc cacggctcga ccatcccgcg tgagtccgac
     7981 gcggtgatct acacccacgc cgggccggag atcgcggtcg cctcgaccaa ggccttcctc
     8041 gcgcagatca ccgcctgcta cctgctgggc ctctacctcg cccaactgcg cggaggtgcg
     8101 ttcgccgacg aggcgcgtgc cgtcatgcgc gacctgcgcg agatgcccga caagatccag
     8161 accgtgctcg accgactggg tcgggtccgc gagatcgccc ggttcatggc cgacacccga
     8221 tcggtgttgt tcctcggtcg ccacgtcggc ttcccagttg ccctcgaagg cgctctcaag
     8281 ctcaaggaga tcgcctacat ccacgcggag ggtttcgcgg cgggcgagct caagcacggg
     8341 ccgatcgccc tcatcgagcc tggccaaccg gtattcgtca tcgtccccgg accggatgcc
     8401 ccgcacgatt tgcatgccaa ggtcgtttcg aacatccagg agatccgggc ccgtggcgct
     8461 cgcacgttgg tcgtcgccca ggacggcgac gaggcggtcg tgccgttcgc cgacgagatc
     8521 atccgggtcc cggccacggc gccgctgctg cagccgttgc tcaccgtcgt tcccctccag
     8581 gtctttgcct gcgagttggc gacggccaag gggctggacg tcgaccagcc gcgtaacctc
     8641 gccaagtccg tgacggtgga gtgattcggt gatcctcggc gtaggcatcg acgtcgtcga
     8701 catcgaccgg ttcggcgcct cgctggagcg cacaccggcc ttgcgccagc ggctgttcac
     8761 cgagagcgag cgggggttgc cgttgcgatc gctggccgcc cgcttcgccg ccaaggaggc
     8821 gttggccaag gcgctcggct cgccggtcgg cctgtcgtgg tccgacgcca ccgtgttgcg
     8881 gggtgaggac ggccgcccgg agttggatgt gcgcgggagc gtcctggccc ggaccaagga
     8941 gttgggcatc cagacgctgc acgtgtcgct gtcccacgac gcggggatcg cctccgccgt
     9001 cgtcgtcgcc gaaggagagc cgtgatccag gcgtatgccg tggccgacgt tcgcgccgtc
     9061 gaggcggccc tgatggagca cctgcccgag ggtgaactca tggcccgagc cgcgagtggg
     9121 ctcgccgagg tcgtccgggc ccggctcgcc gaacgtggtg gacaccgggt cgtcgctctc
     9181 gtgggcggcg gcaacaacgg cgcagatgcc ctgtatgccg cggcgctcct tgcccaggcg
     9241 gggttcgccg tgggtgccgt gtgcacggca tacgggagcg tccaccaggg cgggcgcgac
     9301 gcggcgctgg cggcgggtgt ggtggtgacc ggcggggacg gccctgagtg ggaggccctg
     9361 ttggccgagg ccgacatcgt cgtcgacggg atcacaggga tcggcgggcg gccgggcctg
     9421 ggcgtcgagg cccaaggctg ggtcggggcg atccccgaga cggcctacgt cgtcgcggtc
     9481 gacctgccca gcggcctcga cccggccggc gtggaaggca ccgccgagtg cgtgttcgct
     9541 gacgagacgg tgaccttcgg ggtcgccaaa ccctgccatc tgctgcccgc gaccgaggct
     9601 gccgtgggcc ggctcaccgt ggtcgacatt gggctggact tcgccggttg cacgccggtc
     9661 gtcgagcgcc tgcttggtgc cgacgtggcg gcggcctggc cggtgccgac ggccgcatcg
     9721 gacaaatact cgcggggtgt ggtcggcgtc gtcgccggtt ccgcggccta cccgggtgcg
     9781 ggtgtcctct gcgtcctggg cgcgctcggc accgggccgg ggatggtgcg ctacctcggg
     9841 ccgacctcgg tggcggccct cgtgcatggc cacgcccccg aggcggtgac cgccaccggt
     9901 cgggtccagg cctgggtgct cggctcgggc gtcgaccccg aggacaccga cgacgaggac
     9961 cagttgcggt ggatccgcca ggccctcgac agcgacctgc cgtgcgtcgt cgatgccggg
    10021 gcgctcgccc tgctcggccc gcgctcggcg cccacgctca tcacccctca cgccggcgag
    10081 ttggcgcgcc tgctcacccg gctggccggg gcgggggagg ccgaggtcac ccgcgaggac
    10141 gtcttgggcc gtcccatcga ccacgctcag gccgctgccg atgccttggg cgccgtcgtc
    10201 ctgctcaagg gcgccacaac cctcgtcgtg tctcccaccg ccgccgggag ggcgatccgc
    10261 agccaggccg acgctccgcc gtggctggcc acggccggcg cgggcgacgt gttggccggc
    10321 atcctcggca ccctggtggc cgccggactc gatctcgtga atgcggcgtc cgtgggggcg
    10381 ttcgtccatg gggtggccgc cgatcgagcc aacccgggcg gcccggtgcg tgcgctcgcg
    10441 gtggctcgca aggttcccgg tgtcgtcgcc gccgtgctca ccgcctaact cctcgcaccg
    10501 acgaccccgc gtgccctggc acactgtccc gcgatgactt ccccactgcc cccagcccgc
    10561 cccgggagcc aggcgacggc ctacgtcgac ctgggtgccg tcgagggcaa cgtgcgggcc
    10621 ctgcgcgggc atgcggggcc ggcggcgctc atggcggtcg tcaaggccga cgcttatggt
    10681 cacgggctcg tcccggtggc tcgggccgcc attcgcggcg gtgccacctg gctggggacg
    10741 gcccggttgt ccgaggcgat cacgttgcgg gacaacggga tcccaggtcg gttgatggcc
    10801 tggcaggcgg ttccgggggc cgacttcgcg gcctgcctgg agcgcgagat cgacctcggg
    10861 gtctccgcgc cgtgggcgtt ggacgagatc gtggcggcgg cgcggcatac cgggatcact
    10921 gctcgtgtgc acctcaaggt ggacacgggg ctgggacggg ccggggcgta tgccgcggac
    10981 ttcaccgccc tgctcgacgc agccgtggcc gcggcccggg aggggacggt gcgggtcgtc
    11041 ggggtgtggt cgcacctggc ctgcgccgac gagcctgggc acccgtcgat ccgggcccaa
    11101 cagctggtgt tcgaggccgc ggtggccgag gcagcgcggg ccggctgcga gctcgaggtc
    11161 cgacacctag ccaattcggc tgcgacagtg accgatccga gcttgcacta tgacctcgtg
    11221 cgcaccggga ttgcgatcta cggcctgtcg ccggtgcccg cgctcggtga ctccgcggca
    11281 tacgggctga cgccggcgat gaccctcgta gcgcgactgg ccctcgtcaa ggacgtgccc
    11341 gccggtcagg gggtctccta cggccacctc tacacgacga ccgaggcaac ccggctgggt
    11401 ctcgtcccgc tgggttacgc cgacgggatc ccgcgcaacg caagcaatgt cggcccgctg
    11461 gccgtcggcg gagtccgcca ccgcatcgcc ggacgggtct gcatggacca ggtcgtcgtc
    11521 gacctcgggg ccgggtcgaa cgcccgcgcc ggggatgatg tggtgatctt cggcacgggg
    11581 ggtgcggggg agcccaccgc gcaggagtgg gcggtgtcga ccgggacgat ctcatatgag
    11641 atcgtgacga ggttgggacc gcgggtggag cgggaatacg tcggtgtgga ggaggtcggc
    11701 tagtggcgag aggtcgcccc aacctcgtcg gcctgggtgt cagccttggc gccgccggtg
    11761 ctgctggtgc cctgggtgct gccgggctca tcgccgaccg gatccgccgg cgcgaggacg
    11821 cccaactgcc ggagtactcc agcctcatcg aggcaccgtc cagcgaactc acggtgatcg
    11881 ccgacgacgg ggtgaccctc tacgtcgcca tcgacgaggc caccggcccg acgaagaccg
    11941 cggccgggca cccgaagccg acggtcgttc tctcgcatgg ctactgcctc accagcgagt
    12001 gttgggtgct gcagcgtcgc gcgctgcgcc gggccggcta ccgcgtggtc acctgggacc
    12061 agcgcggaca cggccgctcc ggcatgggcc cgaaggtcgg ctaccacatc gaccaactcg
    12121 gcagggacct gtatgccgtg ctccaggcgg ccgcgcccac cggcgacctc gcgctgatcg
    12181 gccactcgat gggcggcatg acgatcatgg cgctcgccga gagccacccc gacgtgatcc
    12241 gcgaccgcac catcgccttc ggtgccatcg ccacctcacc cggcagcctg cccctggcca
    12301 acggcggctt tgtcgccgcc accggcaagc aggtgctcga acggctcggc ccgagcgtct
    12361 tcactgggct gtccaagcgc cccgagttgc tcggcaccct gctcaaggcc aaccgcgacc
    12421 tggaggagtt cctcgtcgag cgctactcct tcgcctcgcc ggtgccgcgc agcgtcgtcc
    12481 ggctcacggc gcggatgctc atgggcaccg accttgccgt catgtctgac ttcgtcccga
    12541 ccttcgacgc ctacaacaag atccccgcgt tggcgcactt cgcgcacatc gagaccctcg
    12601 tcttcaacgg cacccaggac gtgctcaccc cgccggccca cagcgagttg atcgtcaggg
    12661 gtatccccgg ctccgaacac gtcctcatcc gcgacgccgg gcacgtcatc atgctcgaac
    12721 accccgacct gctcaacgat cagatcctcg cgttgctgga ccgggccgag ttgggccgcg
    12781 ccaagcacct cgacccggcc gagaagccgc acgtcacgct ccacgtcacg ccggtcggca
    12841 aggcgcgccg ggtgcgcgac gggctgcgca acagccgcaa gcgagagcat gtctcctagc
    12901 tcgggaccgc tcacgatccg gctcccggat gccgacgcgg cgcgagcgtt tgggctggcg
    12961 ctggcgaccg tccttcgggc cggtgacgtg ctcgtgctca ccggcgacct cggagcaggg
    13021 aagaccacgc tcacccaagg cctcggtgag ggtctgggcg tgcgagggcc gatcacctcg
    13081 ccgaccttcg tcatcgcgcg ggtgcacccg tcgctggtcg gaggcccggc gctggtccac
    13141 gtcgatgcct atcggctcgg cggcgccgtc gagttggacg acctcgatct cgacgccgat
    13201 ctcgacgcct cggttgtcgt cgtcgaatgg ggtcacgggg tggctgagca gttgagcgcc
    13261 gaccgactcg aggtcgtcct gcaggtcgtc gggccgggcg agacgaggca ggcaaccgtg
    13321 tgggcgaccg gtgagcgctg ggccgacccg accgctcggg ccgccctcgc cgcgctcgcg
    13381 tcaggtgccc cactcgccga cgactagcct tgaccggtgc tcctgctcgc tctcgacacc
    13441 tcgaccagcg ccatcaccgt cgcgctgcac gacggcgacc gagtgctggc tcaggcgacc
    13501 accctcgatg cccgcgccca cggcgagcgg ctcgcacccg gcatacagca ggtgctcgcg
    13561 caggccgggg ccacgccgac tgaactgacc cacgtggtct gcgggctcgg ccccgggccg
    13621 ttcaccgggc tgcgggtcgg catcgtcacc gcccgcgtgc tcgccttcac cacgggcgcg
    13681 gcactgcacg gcatgtgctc gctcgacgcg ctcgccgccg acggaccgac aggcgagatc
    13741 ctcgtcgcga ccgacgccag gcgcaaagag gtctactggg cgcggtatgc cgtctcgccg
    13801 ggttccgtgc gcgccctcac cccggcggct gtcgaccgtc cggccgacct gcctgagcag
    13861 gtgcgggcgc ttccgacgct cggccgaggg ccgctgctct atcccgagtt gttccccgct
    13921 ccggtcggga tcctcgacgt gtcggcagcg gcgctggccg gcctggccgt cagccggctc
    13981 gccgccggcg cggccctgtc cgatcacgag cccctctatc tgcgccgccc cgacgcagcg
    14041 ccgcacatcg tcacgaagtc ggccctcgga tgagtgctcg tgaagtgacg atgcgcgagt
    14101 tgcgctggag cgacatcccc gcgttggccc aggctgagcg cgagttgttc gccgacgacg
    14161 cctggaccga accgacgtgg tgggccgagt tggcggggcg acctcgacgc gactacgtcg
    14221 tcgcggcagc gtcggacggc acgatcgccg gctacgcggg ggtggactgc gccggagaca
    14281 ccgccgacgt catgacgatc gcggtcgaca cggcataccg agggacgggt ctgggggacc
    14341 ggctcctggg tgacctgttg gggcgcgcct cggccgcggg agcgcagtcc gtgctgctgg
    14401 aggtgcgtgc cgacaacgcg ccggcgctcg ggctttatgg acggcacggg ttcgagcagg
    14461 tgtcggtgcg ccggcgctac taccaaccgg gcgatgtcga cgcccttgtc ctgcgtcgga
    14521 ggctgccgtg aacgacgagc cgctcgtcct ggggatcgag acgtcctgcg acgagaccgg
    14581 gatcgggatc gtccggggga gcaccctgct ggtcgacgcc gtggccagtt cggtcgacga
    14641 gcatgcccgc ttcggcgggg tcgtccccga ggtcgcctcg cgggctcacc tcgaggccat
    14701 ggttcccacg ctcgagcggg cctgccgcga tgcgggcgtt gcgctgcgcg acatcgatgc
    14761 tgttgctgtg accgctggcc cgggcctggc cggggccttg ctcgtcggcg ttgccgcggc
    14821 gaaggcgctg gccgtcgggc tgggcaagcc gatctacggg gtcaaccacc tggccgccca
    14881 cgtcgctgtt gacgtccttg agcatggacc cctgccggag ccgacgatgg cgctgctggt
    14941 gtccggtggg cattcgtcgc tgctgctcgt gccggacgtc accggcgatg tccggccgct
    15001 cggggcgacc atcgatgacg cggccgggga ggccttcgac aaggtggctc gggtgttggg
    15061 gctgccgttc cccggaggcc cgcacatcga ccgggtcgcc cgcgagggtg accgggtggc
    15121 gatcgacttc ccgcgtgggt tgacgacagg gcgcgacctc gaccgctatc gcttcgactt
    15181 ctccttctcg ggcctcaaga cggccgtctc acgctgggtg caggtgcggc aggcggcggg
    15241 ggagtcggtg cccgtggccg acgtcgcggc cagcttccag gaggccgtgg tcgacgtgct
    15301 cacccgcaag gccgtcgcgg cctgccggga ccaaggggtc gatgacctgc tcatcggcgg
    15361 tggggtggcg gccaactcgc ggctgcgcgc gctggcgcag gagcggtgcg accgggccgg
    15421 gatccggttg cgggtgcctc ggccggggct gtgcaccgac aacggcgcca tggtggcagc
    15481 gctcggcgcg cagttcgtcg cgcggggccg gacgccgtcg ctgctggacc tcccagccga
    15541 ttcgtcaatg ccggtgacct cggtccaggc ctgagccggc gccgctcggc ccgggtggct
    15601 aaccgccgca gcgcaatagg ccgaacctgg ccttgagggc caggactcga cggaccgact
    15661 gctcgatctc agcggcgaag gcggggtcgc tggcggcccg agccttgatg gcagcgatca
    15721 tgctcggcac ttgcgcggcg ctggcggtga gcaccagatc gccacccgca gcgaggaatc
    15781 gcaccgcccg atcgggcacc gggatcgcgg cgacggcctt ggcggcgccg acgtcatcgg
    15841 tgacgatgac gccgtcgaag cccaggcttg tgcgcagcag gtcggtgacg atcgcccggg
    15901 agaacatcgc cgggttggcg tcgtcgagct tcgggtaacg ggccgaggac accatgacca
    15961 tgccggtgcc ggcagcgatc ccggctcgga aaggctcgag gtaggggtca tccaccgtcg
    16021 cctgcgagtc ggtgatcccc acggcggtga cgtcggtgtt gccggtgatc cggcccagac
    16081 cggggaagtg cttgatcgtc gcgacgacct ggcccgcctg caccgcggtg accagcaccg
    16141 gcacggcggc ggccacgacg gccgggtcgt tccccaactc ccgccggtat ttcccgatcg
    16201 gcgcattggt ctggaccatc gacggcggca cggtgtcggc gaccggtgcg aggttgacgt
    16261 tgacgcccgc gctgcgcaac tccgccgcca gggtggtgcc gagcgccgtc aactcgctcg
    16321 gcggcagttt gccttggtca agcgccgccg gcaggacggt gaagcccttg cccttgagtt
    16381 gttggaccgc tccgccctcc tggtcggcgg cgatgaacag gtcggcaggg ccggtcgcca
    16441 cctgctgcag gtgatcggag gtcgcccgca ccgtgccggc cccggaccag ccaccgagat
    16501 agatcaccga ccccacgtgc gaggcgcgga tcgtcgcgtc gatgccgcca ggggcctgcg
    16561 cgggttggat ccccaccatg atcagctgcc cccagcgttg cgccggggtc atgccgctcg
    16621 cgacctcggc agcgcaggac gccccggccg ttgcggtggg gctcgtggtg ccaccgctcg
    16681 tcgggtatgc cgtcccgccg ggctgggtgc ccgtcgcgcc gctcgcggtg gtcccaccgg
    16741 cactgccccc cgtcgatgct gccccggtcg aaccggtcga accggtcgnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc accggcactg ccccccgtcg atgctgcccc
    16921 ggtcgaaccg gtcgaaccgg tcggtgtcgt cgaacctgcg gtgcaggcgg cggcgagcag
    16981 ggccgcgaga gccaggcccg cggcatacct ggtgttggag agggaccgca tgagtggcga
    17041 ggctacccgc gaccgaagag gagggactcc ccgatcgcca cctggtaggt gaagaacgct
    17101 tcgtggttgg cgatggtctg cggcccgaac tgctcgaaga cctcggaact cacggcgccg
    17161 acgagcatcg cccaggtggc cagaccgttg gccagggtga cggcggacat gccggaggac
    17221 gtgacgtcgg gctcgctgat gaggttcgcg gtcgcggcgg cggcgaggtc ggcggctcgc
    17281 gtggcggggc cgaagggggc tgaaccggcg acaccgcgca gcgcggcgtc gcggccgatc
    17341 gccaggagga gccgcgacac ccgggtgccc ggatcggtgg tctgctccga cggagcctgg
    17401 taggtcggca ccggcgagcc gaacaggagg gcccactcgt gcgggttggc cagcgcccag
    17461 gcccgcatcg acgtggccag tgcggcccat cggccgtgca ggtcggccgg gtcaacggcg
    17521 tcatgggcct cgtcgacggc gtcagcgagg gaggtgtagg cctcgacgat gagcaaggtg
    17581 agcaactcgt cgcggcccga gacgtaacgg aacaacgccg aggggaccat gcccatgtcg
    17641 cgggcgaccg cgcgcaagga cagggccgcc gggccgacct cggccagttg gcgtcgggcg
    17701 gcggctcgca gctcggcctc gatgccggcc cggttgcggg cccggatgcc gtcggcacga
    17761 ccgggggtgg ggcgggggat cgcgggttcg gtcacggtgc cagtgtaggc caacgagagc
    17821 gccgatccgt gtagtgaatg atgttcttga cattgaatgg tgacctgtga tccaatcaga
    17881 gaacgttgat cgcaactcag gacttaccgg cggcttgagg ccgtcggaag ccccaaccag
    17941 aaggagcttc acatgtcccg tcacgtcgtc gtcggagccg gaggcatcgg ccgggccacg
    18001 tccctggcac tcgccgaggc cggtcatgag gtggttctcg cctcgaagtc gggcgttggc
    18061 cgggaactgc ccggggtcaa gcgggtcgcc gtggacgcca ccgatcgccg ggccctcacg
    18121 gctctcgccg aaggcgcggc cagcctggtc aacgcggtca acccgcccac ctacacccac
    18181 tgggaccggg actggccccc gatggcggcc gccttcctca ccgcggcgca ggcgtccggt
    18241 gctggcctgg tcaccatcgg caacctctat ggctacggtc cggtcgacgg gcccatccac
    18301 cccgggctgc cgctcgccgc gaccggcgtc aagagccggg tgcgggccca gatgtgggct
    18361 gacgccctcg ccgcgcacca agccggccgg gtgaaggcca ccgaggttcg ggcgtcggac
    18421 tacttcggcc ccgacatcca accccaggca agcgtcctgc agcgtttcgt cttcggtccc
    18481 gccgccgccg gaaaggccgt gcggcccttc gacggatcgc cggacgcgcc gcactcgtgg
    18541 agctacgtcc ccgacatcgg cgcggtggct gcggccttgg ccaccagcga cctcggttgg
    18601 ggccgcgcat ggcacgtccc ctcaggtatg ccgcgcagcc tcgcccaggc ggccgccgac
    18661 gcggcagcct tcgccggtca cccggcccct gcgatccgac cggttgcgcc ctgggttcgc
    18721 ggactgctgg gcacgttcgt gccggtgatt cgggaactgg acgagacggc ataccagcgc
    18781 gagcgggcgt tcgtcatcga ggcggcggag acggagtcga ccttcggtgt gacggccacc
    18841 ccctggcagg acgctttggc ggcgacgctc accgcgatgg gcgtcgtccc ggcagccgcg
    18901 tcgtcgcgag tgtgagcggg cggcatacgc tgcggccgtg aagcggatcg tcggaggtgg
    18961 tcgctgatgg gccgggtgct tgtgctcggg tcgctcaacg tcgatgtggt ggcgagggtc
    19021 gaacggttgc cccaaccggg ggagaccgtg tcggccgtgg cgtcgatcca gcgcctggcc
    19081 ggcggcaagg gcggcaacca ggcggtggcc gcagccgctg caggcgcgcg cgtcgtcatg
    19141 gtcggcgcgg tcggagacga cgagccgggc cgggcctaca ccgtgcggct ggccggacgc
    19201 ggcatcttcc cgcgtctggc gatcgcgttc ggtgtcccga ccggaaccgc ctatgtctgg
    19261 gttgacgaga acggcgagaa ctccatcgtc gtcgcccctg gagccaacgc cctcgcacgg
    19321 gccgaggtcg ccgacctcac cccggatgac gtcctgctct gccagcttga ggtgccggtg
    19381 gacgtcgtcg ccgacgcggt ccgcacggcc gcccggctcg gtgcccgcgt cgtcctcaac
    19441 gccgcaccgt atgccgccct cccggacgat gtcctcgccg ttgccgatcc tttggtcgtc
    19501 aacgagcacg aggccgggct gctggccgac tcgggagcgg ccgtcggatc gctgctggtg
    19561 accttcgggg cggcaggggc gcagtgggac ggggagcggg tggacggcat acccgtgcct
    19621 gctgatgagg tgctcgactc gaccggtgcg ggggacgcat tctgtggggc gttggcggcg
    19681 cgcctggccg agggcgatga ccgccggtcg gcgctcgcgt gggctgtgcg agcgggggcc
    19741 gatgcggtcc ggcgcgaagg cgcccaaccc gacccggggc tctagacgcc cgtggcggaa
    19801 accgcggcgt cgacaacgat cgccacgggg tggccggcga cggaggtgac gaccaacgtc
    19861 gcgtgacctt cggcgcgggt cgtcaactcg cggcgcagcg cctccgggtc gaccgaaacg
    19921 cctcgcttct tcaccgtcac ccgcccgatg cctcgcgcgc gcagccaggc ccgaaccgcc
    19981 ttgacccgca acggcatcgc ctcgacgacg gcatactgtc gcgcccacgg caggtcgatc
    20041 ggttcggaac ccgtcgcata gcccacgccc gcagccaact cccgcccagg caacgccccc
    20101 gacaaccccg cccgcacgac ggcccggtcc gcctcgtaga accagggacc cacctcgtcg
    20161 agtccgccga tgaccggcgc ttcgcggacg tccgcctcgg tgacgatcgc gggggaggcg
    20221 ccggggcggc ataccgcggc ggtccggccg ggcacctggg cgagtggccc ccaccagatc
    20281 gcgcactcca acacctcgcc ccgccacgat gtccactgcg cctcggtgcc ggacgggagc
    20341 gccgcgtgcg ggaacgcggg cgacagtttc gccccggtcg cgggcacctg cgcggcccag
    20401 gcgagcacct gcggccacgg cggcgagatg gcctccagcg agaacacccg tcgggcccgg
    20461 cccgccgagt cgcgcacgcc gctcacccgc cgccccgggt cgacccagac gccggtatgc
    20521 cgtccgctca cctcccgcgc gcccctcgct ttcgcgaggt cgaccgtctc ggcggacccc
    20581 tccgacacgt gggcgttcgg ccactcggcg aggttcgcgc gggccacggc ggccgtcacc
    20641 gggtcgacct ccacggcctc cactgacaaa cccgaagccg cgaaagccct tgcgtccgaa
    20701 ccgatcccgc atcccaggtc aacgacgtga tccacccccg ccgcgacgaa cctcgacgca
    20761 tgccgcgacg ccaactccgg ccgggtggcc tgctccaacc catcggccgt gaacagcatc
    20821 ccgcctgcag cagctccaaa cttctcgacg gccagccctc gcaaccgggc ctgggccagc
    20881 accgcggtca ccagctccgg ctcgtgcccg gccgcccgca gccgggtcga gagcgcgagc
    20941 gccgagtccg cggcataccc ctcgagcgag tcgagcaacg cgcgacccgg gccggaggcg
    21001 aggcgggcga gcagggcggg gtccatcccc ccatcttccc ggaccacgac ggacggacac
    21061 cgggccggac ctaggctgac gtcatgacgg cccctctgga gggcttgctg gtcgccgact
    21121 tcagtcgcgt cctggccggc cccttcgcga cgatgtacct cgccgacctg ggcgcgaccg
    21181 tcgtcaaggt cgagcggccc gggtctggcg acgacacgcg ggcctggggg ccgccgtgga
    21241 ccgagcacac gagttcctac ttcgagtcgg tcaaccgctc caaactcggg ctcaccctcg
    21301 acctcgccga tcccgctgac caggcgatgg cgcgcgagct cgcccgccgc gcggacatcc
    21361 tcgtcgagaa cttccgcccc ggcaccctcg atcggatggg gctcggttac gagcaggtcc
    21421 gcgacggcga cccggccacc ggccgacccg ccaaccccgg catcgtctac gcctcgatca
    21481 ccggctatgg ctccggggcg ggggcgtcga tcccgggcta cgacttcgtc gtccaggccg
    21541 tcggcgggct gatgagcatc accggcgagc ccgacgcccc ggcccagaag gtcggcgtgg
    21601 ccctcgtgga cgtcctcacc ggcaaggacg cggtcatcgg gatcctcgcc gggatcgccg
    21661 cccgcgcgcg ctccggcgag ggcgtccgcg tcgaggtcga cctgctctcc agcctgctcg
    21721 gggcgctcgt caaccaggcc tccggctatc tcgccaccgg cacgccgccg caacggatgg
    21781 gcaaccgaca cccgtccatc gccccttacg aaacgctggc ctgcgccgat gggcagctcg
    21841 ccgtcgcctg cggcaacgac gggcagttcc gccggctcgc ggccgcggtg gggctggcgc
    21901 acctcgccga cgacgcggca tacgcgacga acagcgcccg cgtcgcccac cgggacgcgc
    21961 tcgcgatcgc cctggagggg cggctgcgcg cgcagccggt cgcccactgg gacggcgtgc
    22021 tgcggggagt gggagtgccg gccgggccgg tcggggacat cgggtccgcc ttcgccctcg
    22081 cgcgggacct cggcctcgag ccgacgtatg ccgcccaccc cggtcacgtg gcccaggtgc
    22141 gcaacccggt ccgcctctcg ggccacgacc caggcccagg tatgccgcct ccgcgcctcg
    22201 gtgagcacga cgccgtggtg cgtggctggc tctccggcgg agatcccagc ctggctcggc
    22261 tggcactcga cttgaccgag tgctaatgag atacctagat ttgcgttagc actctcccct
    22321 ggagagtgct gatcgcacgg ccagcgtcga gccccgcgac ggcgaggccg gccgagcgac
    22381 cacagtcatg acatccatcg acttccgaaa ggacaccgac gtgtcggttt ccatccagcc
    22441 gctcgaggac cgcatcctgg tcaagtccct cgaggccgag accaccaccg catccgggct
    22501 ggtcatcccg gacaccgcca aggaaaagcc gcaggagggc gaggttctcg ccgtcggccc
    22561 cgggcggatg actgacaagg gcgtccgcgt cgccatggac atcaaggtcg gcgaccgggt
    22621 catctacagc aagtacggcg gcaccgagat caagcacggc ggccaggagt tcctcctgct
    22681 gtcggcccgc gacgtcctcg ccatcgtcgg ctgaggcggg tagctcgtgg caaaaacgct
    22741 ggagttcgac gacgccgccc gcaagtccct tgaacggggc gtcgacgcac tcgccaacgc
    22801 cgtcaaggtg acgcttggcc cgaagggccg caacgtcgtc atcgacaaga agtggggcgc
    22861 ccccacgatc accaacgacg gggtgaccat cgcgcgcgag atcgagctcg aggaccccta
    22921 cgagaacctc ggcgctcagc tggccaagga ggtcgccacc aagaccaacg acatcgccgg
    22981 tgacgggacg acgaccgcga cggtcctcgc ccaggcgatg gtcaaggagg gtctgcgcaa
    23041 cgtcgctgcc ggggctgccc cgtcggcgct caagcgcggc atggacaagg ccgtcgaggc
    23101 gctcaacgac cgtctgctgg ccaacgcccg cgagctcgag ggcaccgagg agatcggcca
    23161 ggtcgccgct ctctcggcgc aggacgaggc gatcggcgcc accatcgccg aagccttcga
    23221 caaggtcggc aaggacggcg tcatctcggt cgaggagtcc tcgtcgacgg cgaccgagct
    23281 ggagttcacc gagggcatgc agttcgacaa gggctacatc tcggcctact tcgtcaccga
    23341 cgccgagcgc atggaggccg tcctcgagga cgcctacatc ctcatcaacc agggcaagat
    23401 ctcctcgatc caggacctgc tcccggtgct cgagaaggtc gtccagaccg gcaagccgtt
    23461 ggtcgtcgtg gccgaggacg tcgacgggga ggctctctcc acgctcgtcg tcaacaagat
    23521 ccgcggcacc ttcacggtcg tcgcggtgaa ggcgcccggg ttcggcgacc gccgcaaggc
    23581 gatgctgcag gacctggcca tcctcactgg cggtcaggtc atctccgagg aggtcgggct
    23641 caagctcgac caggccgggc tcgacgtgct cgggcaggct cgccgggtcg tcgtcaccaa
    23701 ggacaccacg accgtcatcg acggtcaggg tggcaaggac gacgtcgacg gccgggtcgc
    23761 tcagatcaag aaggagatcg agcgcaccga ctccgactgg gaccgcgaga agctgcagga
    23821 gcgtctggcc aagctggccg gcggggtctg cgtcatcaag gtcggtgccc acaccgaggt
    23881 tgagctcaaa gaaaagaagc accgcatcga ggacgccatc tcggcgaccc gcgcggcgat
    23941 cgaggagg
//
LOCUS       DAORPW010000020        23844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_340_length_23844_cov_509.685947, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000020 DAORPW010000000
VERSION     DAORPW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 23844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23844
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_340_length_23844_cov_509.685947"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="PLB86_03195"
     CDS             complement(<1..128)
                     /locus_tag="PLB86_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRC64171.1"
                     /translation="MPLESTVKKQIMTEYATVEGDTGSPEVQIAMLTKRIQDLTEHA"
     gene            274..1149
                     /locus_tag="PLB86_03200"
     CDS             274..1149
                     /locus_tag="PLB86_03200"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRC64172.1"
                     /translation="MHDIQWIDLEGVVNMRDVGGMPTLDGGTIASGRLIRSDNLQELS
                     DAAIRRLVDELGVTDVVDLRTHVEVAQEGAGPLVGHPSVQISHFTLYADDSAESGIPD
                     SERELPWETQARREAAARARREQANGDVRGTSGDRSSKPHIVPGGQDHDDFWSDHYLS
                     YLGQRPESVVGALRAIADAKGAVVVHCAAGKDRTGTITGLALVVAGARPEAVIADYAA
                     SAERVPQILDRLLRRPAYAANLLGKTVAEQSPRPETMSRLLAALERDFGGVLGWLRTQ
                     DWTEADTDAVRAKLR"
     assembly_gap    1308..1407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1458..3302)
                     /gene="lepA"
                     /locus_tag="PLB86_03205"
     CDS             complement(1458..3302)
                     /gene="lepA"
                     /locus_tag="PLB86_03205"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRC64173.1"
                     /translation="MARTGLPPHGTPPDRIRNFCIIAHIDHGKSTLADRMLQLTGVVD
                     SRAMRAQYLDRMDIERERGITIKSQAVRMPWESDGVTYALNMIDTPGHVDFTYEVSRS
                     LAACEGAVLLVDAAQGIEAQTLANLYLAMENDLAIIPVLNKIDLPAAQPEKYAAELAG
                     LIGCDPSDCLQVSGKTGVGVEELLDIIVKQVPHPVGDPNAPARAMIFDSVYDTYRGVV
                     TYVRVIDGDLNPRERILMMSTKATHELLEIGVSSPEPEPSKGLGVGEVGYLITGVKDV
                     RQSRVGDTVTNAGKPARESLGGYRDPKPMVFSGLYPIDGSDYPDLREALDKLKLNDAA
                     LAYEPETSAALGFGFRCGFLGLLHLEIVRERLEREFGLDLISTQPNVVYEVTLDDRRV
                     VTVTNPSEYPDGKISEVREPVVRATILAPSEFIGAIMELCQGKRGALRGMDYLSEDRV
                     EMRYSLPLAEIVFDFFDQLKSRTRGYASLDYELDGDQVADLVKVDILLQGEQVDAFSA
                     IVHKDKAYAYGVEMAGKLRKLIPRQQFEVPIQAAIGARIIARETIRAMRKDVLAKCYG
                     GDITRKRKLLEKQKEGKKRMKMVGRVEVPQEAFIAALSSEPIDPKKKP"
     gene            3419..4159
                     /locus_tag="PLB86_03210"
     CDS             3419..4159
                     /locus_tag="PLB86_03210"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HRC64174.1"
                     /translation="MDILVTGGTGTLGRATVPLLQAAGHHVRVLSRRTGPGLTTGDLE
                     RRQGLKRALDGVHTVVHLATSGGARDIRAAANLSKAAVEADVEHIIYMSIVGADRVPL
                     PYYQAKVEAERQLQASGLGLTIQRATQFHELVLTVMRTQRAAPLLFVPDIPIQPVDLS
                     AVAARLTSLIGQEPAGRAPDLGGPRVERFTDLAQQWQHHIDRKRRVVPVRLPGRTFAA
                     YAQGGHLAPEGAATATPTFADFLGAGTV"
     gene            4161..4670
                     /locus_tag="PLB86_03215"
     CDS             4161..4670
                     /locus_tag="PLB86_03215"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRC64175.1"
                     /translation="MGRLRDALARWWRPAAKSPAPRTPTPRTPAPGAPATGYPGDFTG
                     IPDLRWAPRHDGRPDPGEVVWTWVPFEEDHTQGKDRPVLLVGTDGPWLLAVPLTSKDH
                     DRDALQERRDGRLWCDVGSGAWDRRGRPSEARVDRILRVDPTTIRREGAVLDRAVFDQ
                     VAAAIRAAR"
     gene            complement(4761..6428)
                     /locus_tag="PLB86_03220"
     CDS             complement(4761..6428)
                     /locus_tag="PLB86_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC64176.1"
                     /translation="MTTPVTDSPVLSIRNLSIGFRAGGKVAPAVRDVSLDVGVGETVA
                     IVGESGSGKSTTAAAVNGLLPDNAVITSGSILFDGSDIRALTEKQLIAVRGAGIGLVP
                     QDPMSNLNPLMRIGRQIGEALEVHGRTTGRATDQRVHELLELVGIPDPERRATQYPHE
                     FSGGMRQRVLIAIGLSCNPRLLIADEPTSALDVTVQRRILDQLESLTVGSGTAVFLIT
                     HDLALAAERADRVAVMYRGEIVEQGPSKQVLRSPSHDYTKSLIAAVPGLSLERAPVRT
                     AARERISTSDVPLVSVRHVVKEYALRGGSAESFKAVDDVSFDIRAGSTVSVVGESGSG
                     KSTTANMVLGLETITSGNIVFEGNDLTTMRGKELFALRRRVQPVFQNPYSSLDPRFTI
                     ARTIAEPLAVHKVGDPTSRRQRVLELLDQVALPPAVADRYPHELSGGQRQRVAIARAL
                     ALRPDLVVLDEAVSALDVLVQAQILELLASLQREMGLTYLFISHDLAVVRMISDEVHV
                     MRRGKIIESGAPDQLFDHPAHEYTQELIAAIPGRSVASARLGEAEPA"
     gene            complement(6425..7558)
                     /locus_tag="PLB86_03225"
     CDS             complement(6425..7558)
                     /locus_tag="PLB86_03225"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRC64177.1"
                     /translation="MNASTGSELLDAVGTAAHAVHALASGPLGVTIGVTTGAGTRIKS
                     VGAADGNHRALHTDTRFDIASVTKVVATTTAIHRLASVGVLRLDDRVDRFVADSPCTS
                     DATISTLLTHRAGLWEWQPLYLAPSADGRPVDAYEAVAALPLRYPPGERRAYSDLGFI
                     LLGRIIERATGLSLAEAVSEFVTGPLGLVDTAYGPVTGDVAASAPGDGVERDMVGSGD
                     PYPVLYPSREIAWRTHELTGEVNDGNCYRVLGGVSGHAGMFSTAVDLLRLGRSLALAA
                     TSDDLWRPDITAQIFAEGPDSGQALGWRTMPVLLGGVRRNLLWHPGFTGCALGLVPGT
                     GVALVLLSNRLMSAAPLPTAQLWATALSSLGVSDAPMLIGSRP"
     gene            complement(7555..8043)
                     /locus_tag="PLB86_03230"
     CDS             complement(7555..8043)
                     /locus_tag="PLB86_03230"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC64178.1"
                     /translation="MSVQLRRAGLEDRTGIVDVFLDCWRQSYRGVLPDRLTDSMTDEA
                     ATALWNRALTAPDRTVVVAVRDGVVVGVTSYGVNGSTGLVQSLYVSPSAQGLGLGRRL
                     LEKAAVDLTKAAATSATLWVFAANTPSVGFYAACGWLPDGVTRVQEEFGEPEIELHKA
                     LA"
     gene            complement(8040..9209)
                     /locus_tag="PLB86_03235"
     CDS             complement(8040..9209)
                     /locus_tag="PLB86_03235"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRC64179.1"
                     /translation="MRVLGMISGTSHDGIDLAVVDLDADGDVLVGSVLTSDTVPYDPD
                     LRARLVAALPPAATTLSEMCQLDTLIGQAFAQAAAEAAERVGGVDAVCSHGQTVYHWV
                     EADHALGTLQLGQPAWIAERVGRPVVADVRARDLTIGGHGAPLASLLDALLLGGHAGV
                     PAALNLGGISNMTVITDGRVSAFDIGPSNALMDAVVQTEGLDPRGYDDGGRLAAGGSV
                     DVDLLDALLADPYYALPAPKSTGKEHFHGTYVREAVAALGHDISPIDLLATLTELTVR
                     TVAEAVRTAGVTYLAMSGGGCRNPLLVDGIRAALPDVEVVTTDALSVPTDAKEAILLA
                     VIGWCTLHGLPGVVAGGTGARVPRILGSITPGSGPLRLPEPASPIRSIRLHGGVT"
     gene            complement(9191..10141)
                     /gene="murQ"
                     /locus_tag="PLB86_03240"
     CDS             complement(9191..10141)
                     /gene="murQ"
                     /locus_tag="PLB86_03240"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRC64180.1"
                     /translation="MTPAQGDDASDDSDLSALLTESVDPRYADLDILSVAQLAELMNA
                     ADRTVPDAVHAALPQIVLAVEAILPRMIAGGRLVYVGAGTAGRLGILDASECPPTFNT
                     RPEQVFAIIAGGSRAVQTAVEGAEDDPAQGAVAVDAAKVEPRDTVVGITASGRTPYVL
                     GAVKRARELGALTVGLSCDAGSALSGAVERPIEVLVGPEVLSGSTRLKSGTAQKLVLN
                     MISTIVMVRMGKTYGNLMVDVRPTNHKLRVRARRIVQQIASASGDDAAAALAASGNDI
                     RVASLMLARHESAERAAERLRDAGGNLRTALEDGQCASWA"
     gene            complement(10138..11013)
                     /locus_tag="PLB86_03245"
     CDS             complement(10138..11013)
                     /locus_tag="PLB86_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC64181.1"
                     /translation="MTADTMVGAPPRGGQRLRLLLHSRTGMLGLVLVGFLLVLAIAQA
                     FGLVGTDPLKQAASIRLQGPSAAHWFGTDQFGRDVFSRVAAGVANTTQIAAVAVGVAA
                     LVGTLLGVIAGFYLKGPDAIISGVTNVLFAFPPLLLALSLTSVMDRNWFTVAVAIAIV
                     YIPIFIRVARGPVLSLRNIEYVRAAVSTGQSRMAITMRHVLPNITSIIIVQVSLSLSW
                     AVLTEASLSFLGLGNPPPAPSLGSMIYEARTLVSVAPWTMVAPGIVIVLLVVGLNLLG
                     DGLRDSFDPRKRGSS"
     gene            complement(11010..11975)
                     /locus_tag="PLB86_03250"
     CDS             complement(11010..11975)
                     /locus_tag="PLB86_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC64182.1"
                     /translation="MTIPWRTVVRRLLSMMGTLLGVAIFVFIMLRAIPGNQITAGLGT
                     EAAALTPEQRADLEAYYGIDKPLIVQFFSWLGNIATGNLGFSSRSQVSVASLTVDALP
                     VTIELAVLSIILALLIGIPLGMLSASKPDSARDAVGQIFGLAGLSIPSFLLASALLTV
                     LSAKFGFNPNGEAYASLAEDPVRNLRQMLLPSLTLGFGIAAPIMRTTRTALLEVRSQD
                     FVRTARAKGVGNRRLQVRHILGNALVPIVTITGLQFGYLLGGAVVVEQIFSLPGIGRQ
                     VLIGIQGKEYAVVQSTVLVIALLFVVVNLLTDLLYRVIDPRVRAS"
     gene            complement(12040..13455)
                     /locus_tag="PLB86_03255"
     CDS             complement(12040..13455)
                     /locus_tag="PLB86_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC64183.1"
                     /translation="MVGITSDPDNLFPWKATQFQAVNVLQTLYGTLTEFDKDLNVVPG
                     LAESWTYSADNLTLTMKLRAGVKFADGSACDSQDVKFSLDKIKDKATAAVAAASVASV
                     TSVEAPDANTVVLKLTAPDAALLGNLASINMAILPTDSTEAALKTTPAGTGPFILKDR
                     KPSQSITVARNPAYWGDKTPLETVEFRIIPDETSIVSAMQSNNVQLAVLNDPLVAKTA
                     QGGSLTVTKTPQLSYHVLQLNARRPALTDVNVRLAMQCAVDRQQVLDTAALGEGEVIG
                     PITSPAYRSDPSARPCASRDLAKAAEYLAKAGKSDGVSVKTIVSQGEYATSVAEAQNL
                     KAQLAEAKITLDLDVLASNAYVDRWVAGDFDAAVALNGGRPDPDGMYGRYFTSTGNLN
                     KVAGYSSPDLDALFAQGRESTDPTARKATYAKVSQHLEANAAWIWMFSGFTYTVAAPK
                     VTGYLPMANGSVQYLRSTSLP"
     gene            13765..14652
                     /locus_tag="PLB86_03260"
     CDS             13765..14652
                     /locus_tag="PLB86_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRC64184.1"
                     /translation="MTRSEGGQSTALVTMRTLLPNLRASERRIAELFLADPEASAELA
                     IAEVAERSGTSTTSVIRLCKRLGYKGFKDLRNDVLREVTRESFETAAFAGTPRDIDRS
                     DSIEDIIAKVSLAETLSIADTTKVLDVVALGKAADALAAATRIDIFGLGASSFVGEDL
                     QQKLVRIGRAALRWPDSHAAWTAATTLGPGCVAVALSHSGATVDTISFLELARSAGAT
                     TIAITNHSSSPLTEAADIVLTTAARETGFRSGALGSRIAQLMVIDCLFIRVAQLDFDA
                     SMEAIRNTYATIQRLTAKR"
     gene            complement(14693..15067)
                     /locus_tag="PLB86_03265"
     CDS             complement(14693..15067)
                     /locus_tag="PLB86_03265"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC64185.1"
                     /translation="MFVTRGAEAPTFDLPGVRFTPLAAPTLGSADLCLWRLDVDPGLE
                     SPEAHVLDQDEIFVVVAGTISLSIGGMRLTAGDAAIIPAGEPIQLSNPGSEPATVHVA
                     IRSGFAATAADGTSIGTPPWAA"
     gene            15127..15600
                     /locus_tag="PLB86_03270"
     CDS             15127..15600
                     /locus_tag="PLB86_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC64186.1"
                     /translation="MAPAAQPVSDIGISVLLAYQGFVRALHADLAAHGFDDVGKSDGL
                     VFRLLSDRSRTVSELAGLLEVSKQGAGQIIQDMETRGYVVRDQSPGDARARPVRLSDR
                     GLAALAAARRFHRRTEARLVREHGRESVATFRAVLTAMAGDDATALDQQVRGLYF"
     gene            complement(15707..15967)
                     /gene="rpsT"
                     /locus_tag="PLB86_03275"
     CDS             complement(15707..15967)
                     /gene="rpsT"
                     /locus_tag="PLB86_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRC64187.1"
                     /translation="MANIKSQLKRIKTNEKRTERNKAVKSELRTWIRKFRDAAASGDA
                     EAAKSALQAASRKLDKAVSKGVIHANQAANKKSSMARKVASL"
     gene            complement(16167..17162)
                     /gene="holA"
                     /locus_tag="PLB86_03280"
     CDS             complement(16167..17162)
                     /gene="holA"
                     /locus_tag="PLB86_03280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRC64188.1"
                     /translation="MPEVSPLRGRLAGVSVPPFVLITGSEGLLAERALASVLAELRHT
                     EPDLEAIRVAAAAYEGGVLALHASPSLFGGAKAVVVEDFDEGGEELQADVLALVAAPA
                     PDLTLVVMHKSGQRGKKALDALKAARAQVIECPPIKTDRDKADFVTHEFRRQGRKVTP
                     GAVQALVEAVGKDLRELASACSQLVADTVGVVDERVVETYHAGKVEASGFRVADAALA
                     GQTSEALRLLRHAIATGVDPVPIVAVLASQLRTLVKVGAAGRGSSAGIAKTLGMAPWQ
                     VDKARRSISGWDGPGLGVAIQAVAAADVEVKGGGRDPVYAVERAILSIAAARRSG"
     gene            17174..18526
                     /locus_tag="PLB86_03285"
     CDS             17174..18526
                     /locus_tag="PLB86_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HRC64189.1"
                     /translation="MIDRTRLAALMSSEQDRFAAAHPRSAAAYGRADHLFGRVPMTWM
                     NKKAGGFPLYFERGRGSRVWDIDGNDYIDFALGDTGAMAGHSPEPVVAAIRRRVDHLG
                     GLTTMLPTEDAEWVGAELSRRFGMDVWSFSLTATDANRWAIRLVRALTGRPKILVNSY
                     CYHGSVDESLIVVGEDGEGTSRPGNVGSPVDVTMTSRVAEFNDIEGLEKQLAHGDVAA
                     VLMEPALTNIGIVLPDPGYLDAVREVTRRHGTLLIIDETHTFSAGPGGMTQRDRLSPD
                     VVVIGKAIGGGIPTGAYGLSAELAARALSRTDLDLVDMGGVGGTLAGNPLSVAAMRAT
                     LEHVLTDDAFTGMIDTATVFVDGVQSLIDEHHLPWSINQLGARGEYRFAQPYPRNGTQ
                     AAASADGELEDYLHLYLVNRGILLTPFHNMALMAPTTTVADVQRHHEVFAEALGTLPR
                     "
     gene            complement(18529..>18826)
                     /locus_tag="PLB86_03290"
     CDS             complement(18529..>18826)
                     /locus_tag="PLB86_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HRC64190.1"
                     /translation="GDRLRAVPAKRKARVVVLLELLKRFEAGRTYAEPEVNALLRQAH
                     DDVAFLRRELVDYRYLERADGRYWVTTQTPERDANEAQEVPTAEAALLEAMRRS"
     assembly_gap    18827..18926
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19233..21494)
                     /locus_tag="PLB86_03295"
     CDS             complement(19233..21494)
                     /locus_tag="PLB86_03295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRC64191.1"
                     /translation="MSTLDNLLRAAVAGVGGTERPGQVQMAYAVERAVQAREHLLVQA
                     GTGTGKSLAYLVPAVQHALDTGKPSVVATATLALQAQIVDRDLPRLATALAPLLGRSP
                     TFAMVKGRRNYLCLHKLEGGFPDDDALLDVGEVDRAVGRLGQEVIRLRAWAEITESGD
                     RDELVPGVSDRAWRQVSVDAHECLGGKCPLVTACFVERSRAAAAEVDVIVTNHSFMAI
                     DSFEGRQMLPEHDLLVVDEAHELVERVTSTITDELTAGMVSGAARRAGKRSERSDDAT
                     GAADLLGSVLDGMPEGRVLSLPDALVLALSRVRDVARAMQSELKPEAGAEADGALQVA
                     RAAVDEVFENAERILEGRELDVVWLSHEPRRGAVLRVAPMSVAMLVRDKVLADRTVVL
                     TSATLELGGSFDAVAGTMGLRGEGAPGWTGVDVGSPFDYPRQAIAYVAAHLPPPGRDG
                     TPTEAMDELEALVLAAGGRTLGLFSSMRAAQAASEELRDRFSRQGVAIEILCQGEDQI
                     STLVRQFARDSTTCLFGTLSLWQGVDVPGSSCQLVVIDRIPFPRPDDPLASARTQAIA
                     RMGGNGFMAVSATHAALRLAQGAGRLVRRSDDRGVVAFLDSRMTTARYAGFLQRSLPP
                     FWPTTDREKVLAALRRLDEVAPPPLPVAEPTARGLGGAPAAYPMGEEGGEPASDPRPV
                     AGPPPVAPSPRTAVTGGHAWTSAQDAELRDAAEAGIDLDELVEHFELEIDVIQARLAQ
                     LGLEIGEPTFGFE"
     gene            21604..22836
                     /locus_tag="PLB86_03300"
     CDS             21604..22836
                     /locus_tag="PLB86_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC64192.1"
                     /translation="MSGFRERVLPRRLGPAYSLLLGSTWVSNIGDGIALAAGPLLMAS
                     VTRDPLLVAAAAVLQRLPWVLFGLYAGVLADRLDRKRMVVAVDVARAIVLLLFTVALT
                     GGTVHVWLVLAVMFLLGTAETFADTASPTLLPMIVDPPDLGIANARLMAGYLTMNQLI
                     APPIGAALFAIGVAVPFGTQAVCVAFGAVLVGRMKVPPMPPRAEVSHVRRDIAEGFRW
                     LWGNGPVRTLALTILVFNVTWGAAWSVLVLLAQDRLGMGPVGFGALTTAVAVGGLAST
                     AAYDWLERRFTLAALMRVCLLTETLVHLGLALTTMPAVALVVMFAFGAEAFVWGATAR
                     AVRQRAVPTEFQGRVGSVYTLGLFGGLVVGQALGGVLASLWGVTAPFWFAFGGSAILL
                     AIIWRELARIAHGTTTDA"
     gene            complement(22843..>23844)
                     /locus_tag="PLB86_03305"
     CDS             complement(22843..>23844)
                     /locus_tag="PLB86_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64193.1"
                     /translation="TAAAAYEQNGSTGELMLGLLTMEGPQRAVDLAQYLEDSANSLDR
                     QLRDAAMLAGVADSATAVAAERSRVLTEARLARAAALTGMEAELASATDALNAVAGRQ
                     RAMVAELAALQQTSEETQQQRLDGLAAAAAAQAAADAAAQRAAREAAERAAAQAKPSP
                     TPASRATPSTSNTPSTTTSTTSPPRPSASSSSSSSSTPKATSTTTTSTTTPSASASPT
                     STPPPTSSTLAWAQAHPKTVAQQIMPTFGFDSSQWTCLESLWQHESSWNWAADNPYSS
                     AYGIPQALPGTKMATAGADWLVSPATQITWGLGYIKSRYGTPCGAWSAWQSRSPHWY"
BASE COUNT         3667 a         8097 c         8171 g         3709 t
ORIGIN      
        1 gcgtgctcgg tgaggtcctg gatgcgcttg gtgagcatcg cgatctgcac ctcgggagag
       61 ccggtgtcgc cctcgaccgt ggcgtactcg gtcatgatct gcttcttgac tgtgctttcc
      121 agcggcatag ggggccgact ccttctcaag gatgttgcgc ggtgctccag ggcaggtcca
      181 cctgggcgct ctcgatccgc ggccgttcaa cggcgtctct cagactacca gcgcggtccg
      241 agccacccta atcggcggcg taaggtcggt gtcgtgcacg acatccaatg gatcgacctc
      301 gaaggggtcg tcaacatgcg cgacgtcggc gggatgccga cgcttgacgg cgggacgatc
      361 gcatcgggcc gactgatccg ctcggacaac ctgcaggagt tgtccgacgc cgcgatccgc
      421 cgcctcgtcg acgagttggg ggtcaccgac gtcgttgacc tgcgcaccca cgtcgaggtc
      481 gcccaggagg gcgccggacc gctcgtcggt caccccagcg tccagatcag tcacttcacc
      541 ttgtatgccg atgactcggc tgagagcggc atacccgaca gcgagcggga actcccctgg
      601 gagacgcagg cgcgtcgcga ggcagcagcg cgggcgcggc gcgagcaggc caacggcgac
      661 gtccggggca cctcgggtga tcggtcgtcc aagccgcaca tcgtgcccgg cggtcaggat
      721 cacgacgact tctggtccga ccactacctg agctatctgg ggcaacgacc ggaatccgtc
      781 gtcggagccc tgcgcgccat cgccgacgcc aagggtgcgg tggtcgtgca ctgcgcggcc
      841 ggcaaggacc gcaccggaac gatcaccggc ctggccttgg tcgtcgccgg ggctcgaccc
      901 gaagccgtga tcgccgacta cgccgcgtcg gccgagaggg tcccgcagat cctcgaccgc
      961 ctgctgcgcc gaccggcata cgccgcgaat ctgctgggca agacggtggc cgagcagtcc
     1021 ccgcgcccgg agacgatgtc ccggctgctg gccgcgctgg agcgtgactt cggcggggtg
     1081 ctgggctggt tgcgcaccca ggactggacc gaggccgaca ccgacgccgt gcgcgccaaa
     1141 ctccgctgag ccctcccgct ccggttgccc ccagaacctc acactcgccg gtgatttcgc
     1201 gcagttcggc cccgacctgc ccccaagttc cccacacttg ccgacgatct cgggctcagg
     1261 tatgccgccc cgcccccaag ttccccacac tcgccggtga tctcgggnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnggt atgccgcccc gcccccaagt tccccacact
     1441 cgccggtgat ctcggggcta cggcttcttc ttggggtcga tgggctcgct cgagagggca
     1501 gcgatgaagg cttcctgcgg gacttcgacc cgcccgacca tcttcatccg cttcttgccc
     1561 tccttctgct tctccagcag cttgcgcttg cgggtgatgt cgccgccgta acacttggcg
     1621 agcacgtcct tgcgcatcgc gcggatcgtt tcgcgagcga tgatgcgagc gccgatggcc
     1681 gcctgaatcg gcacctcgaa ctgttggcgc gggatgagct tgcgcagctt cccggccatc
     1741 tcgacgccat aggcataggc cttgtccttg tggacgatgg ccgagaaggc gtcgacctgc
     1801 tcgccctgca gcaggatgtc gaccttgacc aggtcggcga cctgatcgcc gtccaactcg
     1861 tagtccagtg acgcatatcc ccgcgtgcgg gacttcaact ggtcgaagaa gtcgaagacg
     1921 atctcggcca gcggcagcga gtagcgcatc tcgacgcggt cctcggacag gtagtccatg
     1981 ccgcgcagtg caccgcgctt gccctggcac aactccatga tcgcgccgat gaactcgctc
     2041 ggcgcgagga tcgtggcccg aacgaccggc tcgcggacct cggagatctt cccgtcggga
     2101 tactcactgg ggttcgtgac cgtgacgacc cgtcgatcat ccaacgtcac ttcatagacg
     2161 acgttgggct gggtcgagat gagatcgagc ccgaactcgc gctccagccg ctcgcggacg
     2221 atctccaggt gcagcaatcc gaggaagcca caccggaagc cgaaccccag cgccgccgag
     2281 gtctccggct cataggccag cgccgcgtcg ttgagcttga gcttgtccag ggcctcccgc
     2341 aggtcggggt agtccgaccc gtcgatggga tacaggccgg agaacaccat cggcttgggg
     2401 tcgcgatagc cgcccagcga ctcacgcgcc ggcttgccgg cattcgtcac ggtgtcgccg
     2461 acccggctct ggcgaacgtc cttgaccccg gtgatgaggt agccgacctc gccgaccccg
     2521 aggcccttgg acggctcggg ctccggcgag gagacaccga tctccagcaa ctcgtgggtg
     2581 gccttggtcg acatcatgag gatccgctcg cgcgggttga ggtcgccgtc gatgactcgg
     2641 acgtaggtga cgaccccgcg gtaggtgtca tagaccgagt cgaagatcat cgcccgagcc
     2701 ggcgcattcg ggtcgccgac cgggtgcggg acctgcttga cgatgatgtc gagcaactcc
     2761 tccacgccga cgccggtctt gcccgagacc tgcaggcagt ccgacgggtc gcagccgatg
     2821 agacccgcga gctccgcggc atacttctcc ggctgcgcgg cagggaggtc gatcttgttg
     2881 agcacgggga tgatcgccag gtcgttctcc atcgcgagat agaggttggc gagggtctgc
     2941 gcctcgatcc cctgggcggc gtcaacgagc aggacggcac cctcgcacgc ggccagcgag
     3001 cgagacactt cgtaggtgaa gtcgacgtgc cccggcgtgt cgatcatgtt gagcgcgtag
     3061 gtcaccccgt cggactccca cggcatccgc acggcctggc tcttgatggt gatgccgcgc
     3121 tcgcgctcga tgtccatccg gtcgagatat tgcgcgcgca tcgcccggct gtcgacgacg
     3181 ccggtgagct ggagcatccg gtcagccaac gtcgacttgc cgtggtcgat gtgggcgatg
     3241 atgcagaagt tgcggatgcg gtccggcggc gtgccgtgcg gcggcaaccc ggtgcgggcc
     3301 atgggtgaca cggtggtggg gttcctcgcg ggcttcgggg gtcgactgga ttcgcaacag
     3361 tgtcccacga cgccgtcggt atgccgtccg ctcaccttcg gcggctagcg tgtcgtctgt
     3421 ggacatcctc gtcaccggcg gaaccgggac cctcggccgg gcgaccgtgc cgctactgca
     3481 agccgccggc caccacgtgc gagtgctcag tcgccgcacc ggaccagggc tgacgaccgg
     3541 ggatctggaa cgccgccaag gtctcaaacg agccctcgac ggcgtccaca ccgtggtgca
     3601 cctggcgacc tcggggggag ctcgcgacat ccgcgcggcc gccaacctct cgaaggcggc
     3661 ggtcgaggcc gacgtcgagc acatcatcta catgtcgatc gtcggcgccg accgggttcc
     3721 cctcccctac taccaggcca aggtcgaggc cgaacggcaa ctgcaagcca gtggcctggg
     3781 cctgaccatc cagcgcgcca cccagttcca cgaactcgtc ctgacggtga tgcgcaccca
     3841 gcgggccgcc ccactgttgt tcgtgcctga catcccgatt caaccggtgg acctctctgc
     3901 tgtcgcggct cgcctcacct cgctcatcgg tcaggaaccg gccgggcgtg ccccggacct
     3961 cggcggtcct cgcgtggagc gcttcaccga tctcgctcag cagtggcagc accacatcga
     4021 ccggaagcgc cgggtcgtcc ccgtccggct gccggggcgc acgttcgcgg catacgccca
     4081 gggcgggcac ctggcacccg agggagcggc gacggcaacc ccgaccttcg cggacttcct
     4141 cggcgccggg acagtctgac gtgggacggc tgcgggacgc gctggcccgg tggtggcggc
     4201 cggcggcgaa gtcacccgct ccgaggaccc ccaccccgag gacacctgcc ccgggcgcgc
     4261 cggcgacggg ctacccgggc gacttcaccg gcatacccga cctgcggtgg gctccgcgcc
     4321 atgacgggcg gcccgatccg ggtgaggttg tctggacctg ggtgccgttc gaggaggatc
     4381 acacccaggg caaggaccgt ccggtcctgc tcgtcggcac cgacgggccg tggctgctcg
     4441 cagtccccct cacctcgaag gaccacgacc gcgacgccct ccaagaacgt cgagacggtc
     4501 ggctctggtg cgatgtcggc tcgggggcgt gggaccgccg cggtcgaccc agcgaggccc
     4561 gcgtcgaccg gatcctgcgc gtggacccga cgacgatccg ccgcgagggc gccgtgctcg
     4621 accgggcagt cttcgatcag gtcgcggcag cgatccgcgc cgcccgctga ccgcaggcgc
     4681 gccgggacag gcgcacggca tacgcgaagg gtcggctccc acacggggag ccgacccttt
     4741 gacgtatgcc gcgtcgtcgc ctacgcgggc tcggcctccc ccagccgcgc actggcaacc
     4801 gaccgcccag ggatcgcggc gatcaactcc tgggtgtatt cgtgcgcggg gtggtcgaag
     4861 agctgatcgg gggccccgct ctcgatgatc ttgccgcgac gcatgacatg gacctcgtcg
     4921 gaaatcatcc gcaccacggc gaggtcgtgg ctgatgaaca ggtaggtgag gcccatctcc
     4981 cgctgcagcg atgcgagcaa ctcaaggatc tgagcctgga cgaggacatc cagcgccgac
     5041 acggcttcgt cgaggaccac caggtcgggg cgcagcgcca gtgcgcgtgc gatggcgacc
     5101 cgctgacgtt ggccaccgga caactcgtgc ggataacggt cggcgacggc cgggggcagg
     5161 gccacttggt ccagcaactc gagcacccgc tgccggcgcg acgtggggtc gccgaccttg
     5221 tgcaccgcca ggggctcggc gatggtgcgg gcaatggtga aacgggggtc gagggaggaa
     5281 tacgggttct ggaagacggg ctgcacccgc cggcgcaggg cgaacaactc cttccctcgc
     5341 atggtggtca ggtcgttgcc ctcgaacacg atgtttccgc tcgtgatggt ctccagaccg
     5401 agcaccatgt tcgccgtggt cgacttgccc gagccggact ccccgaccac cgacacggtg
     5461 ctgccggcgc ggatgtcgaa cgagacatcg tcgaccgcct tgaacgactc ggccgagccg
     5521 ccgcgcaacg catactcctt gaccacgtga cggactgaca cgagcgggac gtcactggtg
     5581 gagatccgtt ctctcgcagc ggtccgcacc ggcgcgcgct ccagcgacag cccaggcacg
     5641 gcagcgatca gtgacttggt gtagtcgtgc gacggactgc gcagcacctg cttggacggg
     5701 ccttgctcga cgatctcccc gcgatacatg accgcgaccc gatcggcgcg ctcggccgcg
     5761 agggcgaggt cgtgggtgat gaggaagacc gccgtgcctg acccgacggt caggctctcc
     5821 aactggtcga ggatgcgccg ctggacggtc acgtcgaggg cggatgtcgg ctcgtcggcg
     5881 atcaggaggc gtgggttgca cgagaggccg atcgcgatga gcacgcgctg gcgcataccg
     5941 ccggagaact catgggggta ttgggtggcc cggcgctctg ggtcggggat gccgaccagt
     6001 tccagcagtt cgtgaacgcg ctggtcggtg gcccggcccg tggtgcgtcc atgcacctcc
     6061 aacgcctcac cgatctgtcg gccgatccgc atgagcggat tgaggttgct catcgggtcc
     6121 tgcggcacca gcccgatccc cgcgccacgc acggcgatga gctgcttctc ggtcagcgcg
     6181 cggatatcgc tgccgtcgaa gagaatgctc ccgctggtga tgaccgcgtt gtccggcagg
     6241 aggccgttca ccgccgcagc ggtggtgctc ttgcccgacc cggactcgcc gacgatggca
     6301 acggtctcgc cgacgcccac gtcgagtgac acgtcgcgga ctgccggtgc aacctttccg
     6361 ccggcacgga acccgatcga cagattgcga atcgacagga cggggctgtc ggtcacgggt
     6421 gtggtcatgg tcggctccct atgagcatcg gggcgtcact gacgccgagt gaggacaacg
     6481 cggtggccca cagctgggcc gtcggcagcg gtgcggcgga catgaggcgg ttggacagca
     6541 agacgagggc aactcccgtg cccgggacca gaccgagcgc acacccggtg aaacccgggt
     6601 gccagagcag gttgcggcgc acgccgccca gcaggaccgg catcgtccgc cacccgagtg
     6661 cctgccccga gtccggcccc tcggcgaaga tctgcgcggt gatgtctggg cgccacaggt
     6721 cgtccgacgt ggctgccagc gccagagatc gacccagccg caggaggtcg acggccgtcg
     6781 agaacatccc ggcgtgcccg ctgacaccgc ccaacacccg gtagcagttg ccgtcgttca
     6841 cctcgccggt cagttcgtgc gtccgccagg cgatctcgcg gctgggatag agcaccggat
     6901 agggatcacc gctgccgacc atgtcccgct cgaccccgtc gccgggtgcg ctcgctgcga
     6961 cgtcacccgt caccggcccg tatgccgtgt ccaccagtcc gaggggaccg gtgacgaact
     7021 cggacaccgc ctcagccaac gacaggcccg tggcccgttc gatgatgcgg ccgagcagga
     7081 tgaagccgag gtccgaatag gctcgacgct cccccggcgg ataacgcagg ggcagggcgg
     7141 ccaccgcctc atacgcatcg acgggtcgac cgtccgcgga cggcgcgagg tagagcggct
     7201 gccactccca caggcccgcc cgatgcgtca ggagggtcga gatcgtggca tccgaagtgc
     7261 agggcgagtc ggcgacgaat cggtcgaccc ggtcatccag acggagcacc ccgacggagg
     7321 caagtcggtg gatcgccgtg gtggtggcca cgaccttcgt caccgaggcg atgtcgaacc
     7381 gcgtgtcggt gtgcagcgcc cgatggttgc cgtcggcggc accgaccgac ttgatcctgg
     7441 tcccggcccc ggtcgtgacg ccgatggtga cgccgagcgg ccccgacgcc agggcatgca
     7501 ctgcgtgggc tgcggtcccg acagcatcga ggagctccga ccccgtcgac gcgttcacgc
     7561 caacgccttg tgcagctcga tctccggttc gccaaactcc tcctgaaccc gcgtcacccc
     7621 gtcgggcagc cacccgcagg ccgcatagaa gccgaccgac ggagtattgg ccgcgaacac
     7681 ccacagcgtc gccgaggtcg ccgcggcctt cgtgagatcg acggcagcct tctcgagcaa
     7741 acggcgtccc aggccgaggc cttgagcact aggcgacaca tagagcgact ggaccaggcc
     7801 cgtggacccg ttcacgccgt aactagtgac cccgacgacg acgccgtccc ggaccgccac
     7861 gacgacggtg cggtccggcg cggtgagggc gcggttccac agcgcggtcg ccgcttcatc
     7921 ggtcatggaa tcggtgaggc ggtcgggaag cacgccgcgg tagctctgcc gccagcagtc
     7981 gaggaagaca tcgacgatcc cggtgcggtc ctccagtccg gcccgacgca gctggacgct
     8041 cacgtgacgc ccccgtgcag tcggatcgag cggatcggcg acgccggctc gggcagccgc
     8101 aagggaccac tcccgggtgt gatgcttccc aggatgcgcg gcacgcgagc cccggtgccc
     8161 ccggcgacga cgccgggcag cccatgcagc gtgcaccagc cgatcacggc gagcaggatt
     8221 gcctcctttg cgtcggtggg gacggagagc gcgtccgtgg tcaccacctc gacgtcaggc
     8281 agggcagctc gtatgccgtc aaccagcaac gggtttcggc acccgccgcc ggacatggcg
     8341 aggtaggtca ccccagcggt ccggacggcc tcggccaccg tgcgcacggt cagctcggtc
     8401 agtgtggcga gcagatcgat ggggctgatg tcgtggccga gggcagcgac ggcttctcgc
     8461 acgtaggtcc cgtggaagtg ctccttgccg gtgctcttcg gcgcgggcaa cgcgtaatag
     8521 gggtctgcga gcaaggcgtc gagaaggtcg acgtcgaccg atccgccggc ggcgagccgg
     8581 cccccgtcgt cgtagcctcg cgggtccagg ccctcggtct gcaccacggc gtccatgagc
     8641 gcgttcgagg ggccgatgtc gaaggccgac acgcgtccat cggtgatgac ggtcatgttc
     8701 gagatgccgc ccaggttcag ggcggccggc acccccgcgt ggccacccaa gaggagcgcg
     8761 tccagaagcg aggcgagggg ggctccgtgc cctccgatcg tcaggtccct ggcgcggacg
     8821 tccgcaacga ccggccgacc gacccgctcg gcgatccacg ccggctggcc gagttgcagc
     8881 gtcccgagtg cgtggtcagc ctcgacccag tggtagacgg tctggccgtg cgagcagacc
     8941 gcgtccacgc cgccgaccct ctcggcggcc tcggcggctg cctgcgcgaa ggcctgcccg
     9001 atgagggtgt cgagctggca catctccgac agcgttgtcg cagcgggtgg cagagcagcg
     9061 acgaggcggg ccctgaggtc ggggtcatag gggacggtgt cgctggtgag gacgctcccc
     9121 accaggacat cgccgtcggc gtccagatcg acgaccgcca ggtcgatgcc gtcgtgtgac
     9181 gtccctgaga tcatgcccaa gacgcgcatt ggccatcctc cagtgcagtg cgtaggttcc
     9241 cgccggcgtc gcgcaggcgt tcggccgcgc gctcggccga ctcgtggcga gcgagcatga
     9301 gggaggccac ccggatgtcg ttcccggaag ctgccaatgc ggcggccgcg tcgtcgccgg
     9361 acgctgaagc gatctgctgg acgatccggc gggcccgcac tcggagcttg tggttggtgg
     9421 ggcggacgtc gaccatgagg ttgccgtagg tcttgcccat gcgcaccatg acgatcgtcg
     9481 agatcatgtt gaggacgagt ttctgcgccg tcccggactt caggcgggtc gagccggaga
     9541 ggacctccgg cccgacaagc acctcgatcg gtcgctcgac cgcaccactc aacgccgacc
     9601 cggcatcgca ggacaggccc acggtgagcg cgccgagctc cctcgcccgc ttgaccgctc
     9661 cgaggacgta gggcgtccgg ccgctggccg tgatccccac gaccgtgtcc ctcggctcga
     9721 ccttggccgc atccaccgct actgcccctt gggcagggtc gtcctcagcc ccttcgactg
     9781 cggtctgcac ggcgcgtgag ccgcccgcaa tgatggcgaa gacctgctcg ggcctggtgt
     9841 tgaaggtcgg cggacactct gacgcgtcga ggattcccag gcgtccggcc gtgccggcac
     9901 cgacgtagac caagcggccc ccggcgatca tccgcggcag gatcgcctca acggcaagga
     9961 cgatctgcgg gagcgcggca tgaaccgcgt ccggcacggt tcgatccgcg gcattcatca
    10021 gctcggccaa ctgcgccacc gacaggatgt cgagatccgc gtatcgcggg tccaccgact
    10081 cggtcaacag cgccgagagg tccgagtcgt cgctcgcgtc gtcgccctgc gcgggggtca
    10141 tgacgatccc cgctttcggg ggtcgaacga gtcgcgcagg ccgtcgccga ggaggttgag
    10201 gcccacgacg agcaggacga tgacgattcc tggggcaacc atcgtccacg gcgcgacgga
    10261 cacgagggtg cgggcctcgt agatcatcga acccagggag ggggccgggg gtgggtttcc
    10321 gagtccgagg aagctcagcg acgcctcggt gagcaccgcc caggacagcg acaacgacac
    10381 ctggacgatg atgatcgacg tgatgttggg cagcacgtgc cgcatcgtga tggccatcct
    10441 gctttggccg gtgctgaccg ccgcgcggac gtattcgatg tttcgcagcg agagcaccgg
    10501 gccacgcgcg acccggatga agatgggtat gtagacgatg gcgatggcca cggcgacggt
    10561 gaaccagttt cggtccatca ccgaggtcag cgacagcgcg agaagcaggg gcgggaaggc
    10621 gaacaacacg ttggtgacgc cgctgatgat ggcgtcgggc cccttgaggt agaagcccgc
    10681 gatgacgccc agcagcgtcc ccacgagagc ggccacaccg accgccaccg cggcgatctg
    10741 ggtcgtgttc gccactccgg ctgcgacgcg ggagaagacg tcacgaccga actgatcggt
    10801 gccgaaccag tgcgctgccg atggcccctg cagtcggatg ctcgcggctt gcttgagcgg
    10861 gtcggtgccg accaggccga acgcttgcgc gatcgccagg accagcagga agccgaccag
    10921 cacgaggccg agcattccgg tgcggctgtg cagaaggagt cgaagacgct gaccgccgcg
    10981 gggcggggcg ccaaccatcg tgtcggcagt catgaggcac gcacccgggg gtcgatgacg
    11041 cggtagagga ggtcagtgag caggttgacg acaacgaaga ggagggcgat gacgagaacc
    11101 gtgctctgga cgacggcata ctccttgcct tggatcccga tgagcacctg ccggccgatg
    11161 ccggggaggc tgaagatctg ctccaccacc acggcgccgc ccagcaagta cccgaactgc
    11221 agaccggtga tcgtgacgat cgggacgagc gcgttgccca ggatgtggcg cacctgcagg
    11281 cgtctgtttc ccacgccctt ggctcgtgcc gtgcgcacga agtcctggga tcgcacctcc
    11341 aacaaggccg tgcgcgtggt cctcatgatg ggagcggcga tcccgaaacc cagcgtgagg
    11401 gagggcagca acatctgccg caggttgcgg accgggtcct cggcgagcga cgcataggcc
    11461 tctccgttgg ggttgaagcc gaacttggcc gagagcaccg tcaacaaggc gctggccaag
    11521 aggaacgacg ggatgctgag gccagcgagc ccgaagatct gtcccactgc gtcccgggcc
    11581 gagtccggtt tcgacgccga gagcatcccc aacggaatgc cgatcagcag cgccaagatg
    11641 atcgacaaca ccgccaactc gatggtgacc ggcagcgcgt cgaccgtcag gctcgcgacg
    11701 ctcacctgtg accgggacga gaaccccaga ttgccggtgg cgatgttccc gagccaggag
    11761 aagaactgaa cgatcagggg cttgtcgatt ccgtaatagg cctcaaggtc ggctcgctgc
    11821 tcgggcgtga gcgcggcggc ctcggtgccg agtcccgcgg tgatctggtt acccgggatc
    11881 gcgcgaagca tgatgaagac gaagatggcg accccaagca gggtgcccat catggagagg
    11941 agccggcgga cgacggtccg ccacgggatg gtcatgggtg cctccgaggc gtggaaagtg
    12001 gcggctgcgg gcccacgggg tggacccgca gccgatccat cagggcagcg aggtcgaacg
    12061 gaggtactgc acggaaccgt tcgccatcgg gaggtacccg gtgaccttcg gcgcggccac
    12121 cgtgtaggtg aagcccgaga acatccagat ccacgcggcg ttggcctcaa ggtgttgcga
    12181 gaccttggcg tacgtggcct tgcgggccgt cgggtcggtc gactcgcggc cttgagcgaa
    12241 cagtgcgtcc aggtccggtg agctgtagcc ggcgaccttg ttgaggttcc ccgtgctcgt
    12301 gaagtagcga ccgtacatac cgtccgggtc cggccgaccg ccgttgaggg cgaccgcggc
    12361 gtcgaagtca ccggccaccc atcgatccac gtaggcgttg gaggccagga catccaggtc
    12421 gagggtgatc ttggcttcgg ccaactgagc cttgaggttc tgggcctcgg cgacggaggt
    12481 ggcgtactcc ccctgcgaca cgatggtctt gacgctcacc ccatcggact tgccggcctt
    12541 cgccagatac tcggcagcct tagcgaggtc gcgcgacgca catggccgag cagacgggtc
    12601 ggagcggtat gccggtgagg tgatcggtcc gatgacctcg ccctctccga gggcggccgt
    12661 gtcgaggacc tgctggcggt cgaccgcaca ctgcatcgcc agacgtacgt tgacgtcggt
    12721 gagggcaggg cgtcgggcgt tcaactgcag gacatgatag ctgagttggg gcgtcttcgt
    12781 cacggtgagg ctgccaccct gggcggtctt ggccaccagc gggtcattca acaccgccag
    12841 ttggacgttg ttcgactgca tcgccgagac gatggaggtc tcgtccggga tgatgcggaa
    12901 ctcgactgtt tccaggggtg tcttgtcgcc ccagtaggcg gggttgcggg ccaccgtgat
    12961 ggactggctc ggcttgcggt ccttgaggat gaatggcccc gtgccggccg gcgtggtctt
    13021 cagcgctgcc tcggtcgagt ctgtcgggag gatggccatg ttgatcgagg ccaggttccc
    13081 gagcagggcg gcgtccggtg cggtgagttt gaggaccacc gtgttcgcat caggtgcctc
    13141 gacgctggtc accgacgcca cggatgccgc agcaacggct gccgtcgcct tgtccttgat
    13201 cttgtccagc gagaacttca cgtcctggga gtcgcaggcg ctcccgtcag caaacttcac
    13261 gccggcgcgc aacttcatcg tcagggtgag gttgtccgcg gagtaggtcc aggactcggc
    13321 caggccggga acgacattga ggtccttgtc gaactccgtg agcgtgccgt acagcgtctg
    13381 taggacgttg acggcctgga actgcgtggc cttccagggg aagagattgt cggggtcgct
    13441 ggtgatccca accaccaggc tcccgccagc tttggacgcg ttggacgacg ctgcgccacc
    13501 cgtgcctgac gagcacgcgg aggcagccat agcgaaggca aggccgagac tgatcaacgg
    13561 aactgagcca cgcgagaggc gtgtacgacg agccatcaga actcctttcg aaccgagccg
    13621 ttgtcgttgg tcctccgtag gctggaggtg acgccgggtg gtaggtcgct aacttacatg
    13681 tcgacggttc gaaatgtact agacttacct cctcttgact agagtctggt gaccaccaag
    13741 acgggaagtc ctatgaccgc atcgatgaca cgctccgagg gggggcagtc caccgccctc
    13801 gtgaccatgc gcaccctgct cccaaacctg cgggccagtg agcggcgcat cgcggagctc
    13861 ttccttgccg accccgaagc gtcggccgaa ctggccatcg ccgaggtcgc cgaacgcagc
    13921 gggacctcaa cgacctcagt gatccgcttg tgcaagcgac tgggctacaa ggggttcaag
    13981 gacctgcgca acgacgtgct gcgtgaggtc acccgggagt ccttcgagac ggcggcgttc
    14041 gccggcaccc cgcgcgacat cgatcgctcg gacagcatcg aggacatcat cgccaaggtc
    14101 tcgctcgccg agacactgtc catcgccgac accaccaagg tcctggacgt cgtcgcccta
    14161 gggaaggccg ccgatgctct cgcggctgcc actcgcatcg acatcttcgg cctcggcgcc
    14221 agttccttcg tcggcgagga cctccaacag aaactggtgc ggatcgggcg agcagcgctt
    14281 cgctggcccg actcccacgc ggcgtggaca gccgccacca ccttgggtcc gggctgcgtg
    14341 gctgtcgcct tgtcccactc gggcgccacc gtggacacca tctcgttcct ggagctcgcc
    14401 cgcagtgcgg gcgccacgac catcgccatc accaaccaca gcagctcccc cctcaccgag
    14461 gccgccgaca tcgtgctgac cacggctgcc cgggagaccg gtttccggtc cggcgccctg
    14521 ggcagccgca ttgctcaact catggtcatc gactgcctgt tcatccgggt ggcccagctg
    14581 gacttcgacg cgtccatgga ggccatccgt aacacctacg ccacgatcca aaggctcacg
    14641 gcgaaacgct agagccagtg gtcgatgctt ggcggatcag ggcggtctac ggtcaggccg
    14701 cccagggcgg cgtccctatc gacgtgccgt ccgcggcggt cgcggcgaaa ccagaccgga
    14761 tcgccacatg caccgtggcc ggctccgagc ccgggttgct caactgaatc ggctcgccgg
    14821 cggggatgat cgcagcgtcg ccggcggtca gacgcatgcc accgatcgac agggagatcg
    14881 tgcctgcgac aaccacgaag atctcgtcct gatcgagcac gtgggcctca ggggactcca
    14941 gcccggggtc gacgtccagg cgccagaggc acaggtctgc cgatcccagc gtcggcgcgg
    15001 cgagcggcgt gaagcgcact ccgggcaggt cgaaggtcgg cgcctcggcg cctcgagtga
    15061 caaacatgtt cgctctcctc gatagacaag ttcacttgtc caagtgaact tgtctatcat
    15121 gaggttatgg cacccgctgc gcaacccgtt tcagacatcg gcatctcggt cttgctggcc
    15181 taccaggggt ttgtgcgcgc tttgcacgcc gatctggctg cccacggctt cgatgacgtg
    15241 gggaagtctg acggcctggt ctttcgtctc ctgagcgatc gctcgcgcac ggtgagtgag
    15301 cttgctgggc tcctggaggt gtccaagcag ggggccgggc agatcatcca ggacatggag
    15361 acgcgcggct acgtcgtgcg cgaccagagc cccggcgatg ctcgcgcccg gcccgtgcgc
    15421 ctcagtgacc gtggcttggc tgcgcttgcc gcggctcggc ggtttcatcg ccgcaccgag
    15481 gctcgcctgg ttcgcgaaca cggcagggaa tcggtggcta ccttcagagc ggtgctcacg
    15541 gcaatggccg gcgacgacgc cacagctttg gatcagcagg tccgggggct ctacttctag
    15601 gggttcccta gtcgaccgtc gcctgaaacc cgccactggc gcgagacgca aagggtcggc
    15661 tcccacacgg ggagccgacc ctttgacgta tgccgcgtcg tcgcctttag aggctggcga
    15721 ccttgcgggc catcgaggac ttcttgttgg ctgcctggtt ggcgtggatg acgcccttgg
    15781 agacggcctt gtcgagcttg cggctggcgg cctgcagggc ggacttagcc gcctcggcgt
    15841 caccggaggc cgcggcgtcg cggaacttgc ggatccacgt gcgcagctcg ctcttgaccg
    15901 ccttgttgcg ctcggtgcgc ttctcgttgg tcttgatgcg cttgagctga gacttgatgt
    15961 ttgccactgg tgatcggttc tttcggtcgg tgcgggtatg ccgttagagg gcggcagacg
    16021 cggctcggga ccgggcgtgg ggacacccgt ggttgagtcg cgctgggtgg tgcgatcgtg
    16081 cgcgcacgac caggcgcgca cgcaaggggt caatctagca gcgggcgggc cgcccttccc
    16141 aattggcgcg cccaacgagc ctggggtcag ccggaccgac gggcggcggc gatggagagg
    16201 atggcgcgct ccacggcata caccgggtcg cggcccccac ccttgacctc gacgtcggcg
    16261 gccgcgaccg cctggatggc gacgcccagc ccgggcccgt cccatccgga aatcgatcgg
    16321 cgggccttgt caacctgcca gggagccatg ccgagggttt tggcgatccc ggccgacgac
    16381 ccgcgcccgg ccgcacccac cttgaccagg gtgcgcaatt gcgaggcgag gacggcgacg
    16441 atcggcaccg ggtcgacgcc ggtcgcgatc gcgtggcgca gcagccgcag tgcctcgctc
    16501 gtctgaccgg ccagagcggc gtcggcgacc cggaagccgc tcgcctccac cttgcccgcg
    16561 tgataggtct cgaccacccg ctcatcgacg accccgaccg tgtcggcgac cagttggctg
    16621 caggccgacg cgagctcgcg cagatccttg ccgaccgcct cgaccagcgc ctgaacggca
    16681 cccggcgtga ccttgcgacc ctgcctgcgg aactcatggg tgacgaagtc ggccttgtct
    16741 cggtcggtct tgatcggcgg gcactcgatg acctgagccc tggccgcctt caacgcgtcg
    16801 agagccttct tgccgcgctg accggacttg tgcatgacga cgagcgtgag gtcgggtgcc
    16861 ggtgcggcga ccaacgcgag gacgtcagcc tgcaactcct cgccgccctc gtcgaagtcc
    16921 tcgacgacga ccgccttcgc cccgccgaag agcgacgggc tcgcatgcag ggccagaacc
    16981 ccgccctcgt atgccgcagc agccacccgg atcgcttcga ggtccggctc ggtgtggcgc
    17041 agctcggcga gcaccgaggc cagggcgcgc tcggcgagca gcccttcaga gccggtgatg
    17101 aggacgaacg gcggaacact cacgcccgca agcctcccac gcagcggtga cacctcgggc
    17161 atgatggcct cccatgatcg accgcacccg cctagccgcc ctcatgtcgt ccgagcagga
    17221 ccgcttcgcc gccgcccatc cgcgctccgc ggcggcatac ggccgggctg accacctctt
    17281 cgggcgggtg ccgatgacct ggatgaacaa gaaagcgggc ggcttcccgc tctatttcga
    17341 gcgcggtcgc gggagccggg tgtgggacat cgacgggaac gactacatcg acttcgccct
    17401 gggtgacacc ggcgcgatgg cggggcactc ccccgagccg gtcgttgccg ccattcgacg
    17461 gcgagtcgat cacctcggcg ggctgacgac gatgctcccc accgaggatg ccgaatgggt
    17521 gggcgccgag ttgtcgcgcc ggttcgggat ggacgtctgg agcttctcgc tcaccgcgac
    17581 cgacgccaac cgttgggcga tccggctggt gcgagccctc accgggcgac cgaagatcct
    17641 cgtcaacagc tactgctacc acgggtcggt cgacgagtcg ctcatcgtcg tcggcgaaga
    17701 tggcgagggc accagccggc cgggcaacgt cggttcgccg gtcgatgtga cgatgaccag
    17761 ccgggtggcc gagttcaacg acatcgaggg cctggagaag caactggctc acggtgatgt
    17821 ggcggcggtg ttgatggagc ctgcgctgac caacatcggc atcgtcctgc cggacccggg
    17881 ctacctcgac gccgtccgcg aggtgacccg tcgacacggc accctgctca tcatcgacga
    17941 gacgcatacc ttctcggcag ggccgggcgg gatgacccag cgggatcggc tctcccccga
    18001 cgtcgtcgtc atcgggaagg cgatcggcgg cggcataccg acgggggcct atggcctgtc
    18061 cgccgagttg gccgcgaggg ccctgtcccg gaccgacctc gacctggtcg atatgggcgg
    18121 ggtgggcggc accctcgccg gcaacccgct gtcggtggca gcgatgcggg cgaccctcga
    18181 gcacgtgctg accgacgacg cgttcaccgg gatgatcgac accgcaacgg tgttcgtcga
    18241 cggtgtgcag tcgctcatcg acgagcacca cctgccgtgg tcgatcaacc agctgggcgc
    18301 ccgcggtgaa taccgctttg cccagccgta cccacgcaac ggaacgcagg cggcggcatc
    18361 ggccgacggt gagctcgagg actatctgca cctctacctg gtcaaccgcg ggatcctcct
    18421 aacgccattc cacaacatgg cgctcatggc tccgacgacg acggttgccg acgtccagcg
    18481 tcaccacgag gtctttgccg aggccttggg caccctgccc cgctgacgct aggaccggcg
    18541 catcgcctcc aggagggccg cctcggcggt cggcacctcc tgcgcctcgt tggcgtcccg
    18601 ctccggtgtc tgggtggtga cccagtagcg gccgtccgcg cgctcgagat agcggtagtc
    18661 gaccaactcc cgacgcagga aggccacgtc gtcgtgggcc tgtcgcagca gcgcgttgac
    18721 ctccggctcg gcgtaggtcc ggcctgcctc gaagcgtttg agcagctcca ggagcacgac
    18781 gaccctcgcc ttgcgcttgg ccgggacggc gcgcagccga tcgccgnnnn nnnnnnnnnn
    18841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnncggc gatcacgtcg gcgcgggcgg cggtgtaggt
    18961 ctggccggtc agggccatcc gggcgcggac gagggccttg aaatcggaat cggtggtcat
    19021 cgggaatcgc tctccggctg tgcacggccc cagcccgccg cagcctgtgg ggcgaacgcg
    19081 acgacagcat gtcagcccga ggatgtgctc cccttcgccg tcatgtgcgc aggctggggc
    19141 cggcaccggc tgggcgtcag gttacgccgc tcgggtcagc ctggcatcgg acggccgccc
    19201 aggtcaaccg agttgcgccg aagcgcagcg cgtcactcga aaccgaaggt cggctccccg
    19261 atctccagcc ccagttgcgc cagccgcgcc tggatgacgt cgatctccaa ctcgaagtgc
    19321 tcgaccaact cgtccaggtc gatccccgcc tcggcagcgt cccgcagctc ggcatcctgc
    19381 gccgacgtcc aggcatgacc gccggtgaca gccgtccgcg gtgagggcgc gacgggcggg
    19441 ggtccggcca ctgggcgcgg atcgctcgcc ggctcgccgc cctcctcccc catcgggtat
    19501 gccgcgggcg cacctcccag cccccgggcc gtgggctcgg cgaccggcaa cggcggtgga
    19561 gcgacctcat cgaggcggcg cagggcagcc agcaccttct cccgatcggt cgtcggccag
    19621 aagggaggca gcgagcgttg gaggaagccg gcatacctcg cggtggtcat ccgggagtcg
    19681 aggaaggcga caacgccccg gtcgtcggag cggcggacaa gccggccggc gccttgcgcc
    19741 aatcgcaacg ccgcatgggt ggccgacact gccatgaacc cgttgccgcc catccgggcg
    19801 atcgcctgag tccgtgccga ggccaatggg tcatccggtc gcgggaaggg gatccggtcg
    19861 atgacgacca actggcacga cgaccccggc acgtcgacgc cctgccacag actcaaggtc
    19921 ccgaacaggc acgtcgtcga gtcacgtgcg aattgccgta ccagagtgga gatctggtct
    19981 tcaccctggc acaggatctc gatggccacc ccctgacggg agaagcggtc gcgcagctcc
    20041 tccgaggccg cctgggcggc ccgcatcgag gagaacagcc cgagcgtccg gccgccggcg
    20101 gccagcacca gggcctccag ctcgtccatc gcctccgtcg gggtgccgtc ccgaccgggt
    20161 ggcggcaggt gggccgcgac ataggcgatc gcctggcgcg ggtagtcgaa cggcgagccg
    20221 acatcgacgc cggtccaccc gggcgcaccc tcgccgcgca gccccatcgt cccggccacc
    20281 gcgtcgaagg atccccccaa ctccagcgtc gccgaggtca gcacgacggt ccggtcggcg
    20341 agcaccttgt cgcgcaccag catcgccacg ctcatcggag cgacccgcag cacggccccg
    20401 cggcgcggtt catgggagag ccagaccacg tcgagctcgc ggccctcgag gatccgctcg
    20461 gcgttctcga acacctcgtc gaccgcggcc cgcgcgacct gcagcgcccc gtccgcctcg
    20521 gccccggcct caggcttgag ctcactctgc atcgcccggg cgacatcccg gacccggctc
    20581 agggccagga cgagcgcatc gggcaggctg agaacccggc cctccggcat accgtcaagg
    20641 accgacccga gcaggtccgc cgccccggtc gcgtcgtcgg accgctcgct gcgcttgccg
    20701 gcccgccggg ccgcgccgct caccatgccc gcggtcagtt cgtccgtgat cgtcgaggtg
    20761 acccgctcga ccaactcgtg cgcctcgtcg acgacaagca gatcgtgctc gggcaacatc
    20821 tggcgacctt cgaacgagtc gatcgccatg aacgagtggt tggtgacgat gacgtcgacc
    20881 tcggccgccg cggcccgcga gcgctccacg aagcaggcgg tgaccagcgg acacttcccg
    20941 cccaggcact cgtgggcgtc gaccgacacc tgccgccagg cccggtcgga cacgcccggg
    21001 acgagttcgt cgcggtcgcc ggactcggtg atctccgccc acgcgcgcag ccggatgacc
    21061 tcctgcccca gccgccccac cgcacggtcg acctcgccga cgtcaagcag ggcgtcgtcg
    21121 tcggggaagc cgccttcgag cttgtgcaga cacagatagt tgcgccggcc cttgaccatg
    21181 gcgaaggtcg gcgagcgccc cagcagcggc gccaaggcgg tcgccagccg gggcaggtcg
    21241 cggtcgacga tctgggcctg cagcgccaga gtggcggtgg cgacaacgga gggcttgccg
    21301 gtgtcgaggg cgtgctggac cgcgggcacg aggtaggcca gcgacttgcc ggtgccggtc
    21361 ccggcctgga cgagcaggtg ctcgcgggcc tggacggcgc gctccacggc atacgccatc
    21421 tgcacctggc ccggccgctc ggtgcccccg actccggcca ccgcagcgcg cagcaggttg
    21481 tcgagggtgg acaccgcgcc actgtatgcc gtgccgcccg ccactcgcgg cagccgtccc
    21541 gggccgggcc acgtggcaaa ccccacgcgg tgcggccgcc gagacgggta gcgtcatcgg
    21601 tttgtgagcg gctttcggga gcgggtgcta ccgcgtcgcc tcggcccggc ctactccctg
    21661 ctgctcgggt cgacctgggt aagcaacatc ggcgatggca tcgccctcgc ggccggcccc
    21721 ttgctcatgg cgtcggtgac ccgcgatcct ctgctcgtgg ccgcggccgc cgtgctccag
    21781 cgactgccgt gggtgctgtt cggcctgtat gccggtgtgc tggctgatcg tctcgaccgc
    21841 aagcgcatgg tcgtcgccgt cgacgtggct cgggcgatcg tgctgctcct cttcaccgtg
    21901 gcgctcaccg gtgggacggt gcacgtctgg ctcgtcctgg ccgtgatgtt cctgctcggg
    21961 acggccgaga ccttcgcgga caccgcgtca ccgacgctgc tgccgatgat cgtcgaccca
    22021 cccgacctgg gcatcgccaa cgcccgcctc atggcgggtt acctgacgat gaaccagttg
    22081 atcgctccgc cgatcggtgc ggcgttgttc gcgattggcg tcgccgttcc cttcgggacc
    22141 caagccgtgt gcgtggcctt tggtgcggtg ctcgtcgggc ggatgaaggt gccgcctatg
    22201 ccgccgcgag ctgaggtcag ccacgtgcgg cgggacatcg ccgagggttt ccgttggctc
    22261 tggggtaacg ggccggtccg aaccctggcc ctcacgatcc tcgtcttcaa cgtgacatgg
    22321 ggcgccgcgt ggtcggtcct cgtcctcctg gcccaggacc gactcggcat ggggccggtg
    22381 ggattcggtg ccctgacgac ggccgtggcg gtcggcgggt tggcgagcac cgcggcatac
    22441 gactggttgg agcggcggtt caccctcgct gccctcatgc gggtgtgcct gctcaccgag
    22501 accctggtcc atctcgggct ggccctgacg acgatgccgg cagtggcgct ggtggtgatg
    22561 ttcgccttcg gtgcggaggc gttcgtctgg ggcgcgacgg ctcgggcggt gcgccagcgg
    22621 gcggttccca cggagttcca ggggcgggtc ggaagcgtct acaccctcgg gctgtttggc
    22681 gggttagtcg tggggcaggc cctcggcggc gtcctcgcct cactgtgggg cgtgacggcg
    22741 ccgttctggt tcgccttcgg cgggtcggcg atcctgctcg ccatcatctg gcgcgagttg
    22801 gcccgaatcg cgcacggcac gacgaccgac gcctgacgcc gatcagtacc aatgcggaga
    22861 acgagattgc caggcgctcc aggcaccgca gggagttccg taccgactct tgatgtaccc
    22921 caacccccag gtgatctggg tcgccgggct gaccaaccag tcggcgcccg cggtggccat
    22981 cttcgtgccg ggcaacgcct gcgggatgcc gtaggcgctc gagtacgggt tgtccgccgc
    23041 ccagttccag ctgctctcgt gctgccagag cgactcgaga cacgtccatt ggctggagtc
    23101 gaagccgaac gtcggcatga tctgctgcgc cacggtcttg ggatgcgcct gggcccacgc
    23161 cagggtcgag gacgtcggcg gcggcgtgct cgtcggggac gcggaggctg acggggtggt
    23221 cgtcgacgtc gttgtggtcg aggtggcctt cggcgtggag gacgacgagg acgacgagct
    23281 cgccgacggg cgcgggggcg aggtcgtcga ggtcgttgtc gacggcgtgt tcgacgtcga
    23341 cggcgtggcc cgcgaggcgg gagtcgggct cggcttcgct tgggctgcag cgcgttcggc
    23401 cgcctcccga gcggcccgct gcgcggctgc gtcagcggct gcctgcgccg cagcggctgc
    23461 cgccagcccg tccagccgct gctgctgggt ctcctcggag gtctgctgaa gcgctgctag
    23521 ctcggccacc atcgctcgct gtcggccagc gacggcattg agcgcgtcgg tcgccgaagc
    23581 gagttccgcc tccatgccgg tgagggcagc ggcccgcgcc agtcgggcct cggtcaacac
    23641 gcggctgcgc tcagcggcaa cagcggtcgc cgagtcggcg accccggcga gcatcgcggc
    23701 atcgcgcagt tgacggtcca ggctgttggc ggagtcctcg aggtattgcg caaggtccac
    23761 cgctcgttgt ggcccctcca tggtgaggag tcccagcatg agttctccgg tcgacccgtt
    23821 ctgctcgtat gccgcggcgg ccgt
//
LOCUS       DAORPW010000021        23482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_349_length_23482_cov_794.611474, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000021 DAORPW010000000
VERSION     DAORPW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 23482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23482
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_349_length_23482_cov_794.611474"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1701)
                     /locus_tag="PLB86_03310"
     CDS             complement(316..1701)
                     /locus_tag="PLB86_03310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC64194.1"
                     /translation="MTRPLWWEGGIPSPAAAPGVGPVGAGYAAQPPSEVDVAIVGGGF
                     TGLWTAYYLLGLDPSLSVVVLEAEHVGFGASGRNGGWVSALWPVSASTLARESGDAAA
                     RSMGAALRETVLEVGRVAADEGIDCDYRRGGVVLAARTPAQVERARGYAAAGAAWGDG
                     TVWLDLPEARDRVRMSGLLGATYNPNCARIHPRKLVDGLAAVVRSRGGLIVEGARVTS
                     ASPGRVVLESGTVVRTRHTVRATEAWTARLAGLRRRTTPVYSLMIATEPLPAGVWDEI
                     GLAQRETFADHRHVIIYGQRTADDRIAFGGRGAPYHWGSEIRPEFDSEPTVFAELEST
                     LRGLFPVLQGYAVTHRWGGPLGIARDWHPSVGYDPVARLGWAGGYVGDGVAASNLAGR
                     TLADLVLGRASGLTRLPWVGHVSPSWEPEPVRWLGVNAGLRVAGWADLEESLTGREAV
                     LGRVLSALTGH"
     gene            complement(1698..3128)
                     /locus_tag="PLB86_03315"
     CDS             complement(1698..3128)
                     /locus_tag="PLB86_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRC64195.1"
                     /translation="MTIYPSEIWEPSSDFHTGTGKLYSEAARDHLWMHFTRHSVFEDR
                     DGAHTVPLIVKGDGAYIWDDRGNKYLDGLSGLFTVQVGHGRQELADAAMAQFQKIAYF
                     PLWSYAHPRAVELAERLAHYAPGDLNRVFFTTGGGEAVESAWKLAKQYYKLVGKPMKT
                     KVISRNIAYHGTPQGALSITGIPAAKAVFEPLVPGGHKVPNTNLYRAPEHLRDDPKAF
                     GLWAANRIAEAIEFEGPETVAAVFLEPVQNSGGCFPPPPGYFERVREICDEYDVLLVS
                     DETICAFGRIGDYFACNQLGYVPDIITTAKGVTSGYAPLGCMIVSDRLFEPFRHGQTY
                     FPHGYTFGGHPISCAVAMANLDIFEKEGLNAHVRETAPAFRASLETLLDLPIVGDVRG
                     EGFFYGIELVKDKATRETFDDGECERLLRGYLSKALFDAGIYCRADDRGDPVIQLAPP
                     LIIGQPEFDDITSRLRSVLQGAEAHL"
     gene            complement(3125..3601)
                     /locus_tag="PLB86_03320"
     CDS             complement(3125..3601)
                     /locus_tag="PLB86_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRC64196.1"
                     /translation="MRRSTRRPTRQVLDATAKAIIEQLQEDGRRSYAAIATAVGLSEA
                     AVRQRVQKLVDGGVVQIVAVTDPLHVGFLRQAMVGIKVQGDIGPVAEAVSAIPEVDYV
                     VTTAGSFDLLAEVVCEDDAHLLEVISQRIRTLPGVGATETFVYLELNKQHYNWGTR"
     gene            3822..5270
                     /locus_tag="PLB86_03325"
     CDS             3822..5270
                     /locus_tag="PLB86_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HRC64197.1"
                     /translation="MSQAPRVLRNHVGGEFVDATADARSDVVNPATGQVVATAPVSGQ
                     EDVNRAYAAASAAFEEWGRTTPRERQEALLKFAAAVEARADDFVHLEGENTGKPHALT
                     ASEEVPPMLDQIRFFAGAARVLEGRSAGEYMPGHTSWIRREPIGVIGQVTPWNYPMMM
                     AIWKIAPALAAGNTVVLKPSDTTPETTLLLAALAADCFPAGTFNVVTGDRETGRMVVS
                     NPRADMVAITGSVRAGSQVAAAAAESVKRVHLELGGKAPVVVFDDADIEAAVEGIAIA
                     GYFNAGQDCTAATRVLAGPGIYDDFVAALGEYAANSAKVGMPDDEDALFGPVNNPAQL
                     ERVTGFLNRVPAHASVVAGGSRVTDLGDGYFIAPTVLAGLLQDDEHIQNEIFGPVITV
                     QRFSDEDEAIRWANGVDYGLASSVWTKDFGRAMRMSRALDFGCVWINTHIPIVAEMPH
                     GGYKHSGYGKDLSMYGLEDYTRIKHVMANIQA"
     gene            5400..6641
                     /locus_tag="PLB86_03330"
     CDS             5400..6641
                     /locus_tag="PLB86_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRC64198.1"
                     /translation="MRRSEPPRDPQARALASLAASSLSRRRLLSGAGLGLAGAGLAAC
                     APPLPPAATGPTGPKLPVDKSATDKTVAFANWTAYLDYDEEAKNYPTLEAFIKETGIQ
                     ASYTEDIEDNDAYVSKIKPQLQATPPQDINRDIFALTDWMANRVIRDGLVQPLDLIRM
                     PHAANLLDSLKDVSFDPGRKYSLTWQSGFAGIGYDKSKVGRELKSLSDLWADDLKGKV
                     VALSEMRDTLGLIMQSQGVDISGSFTKDQVSKAMDEVSKRIADQNIRRIKGNSYMEDL
                     KSGNAIAGIVWSGDLFILRAETENENWEFVIPDSGGTLWSDNMMIPITSTHRRNAMAL
                     MDYYYRPDVAAQVAAYVNYVCPVVGAQAELAKTDPELAESPFIFPTSEWIAEHKIQGF
                     RALDAQEDADYSAMWAKVVGN"
     gene            6641..7741
                     /locus_tag="PLB86_03335"
     CDS             6641..7741
                     /locus_tag="PLB86_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC64199.1"
                     /translation="MAEFRAAKGDLVLENVTKSFTNFTAVDDLSLTVPRGAFFALLGP
                     SGCGKTTTLRMVAGLEQPTSGRVLIGDTDLTGSRPYERPVNTVFQSYALFPHLTVRDN
                     VAFGPKRRGAKDAQAQADATLALVQLTHLAGRKPTQLSGGQQQRVAVARALVNHPEVL
                     LLDEPLGALDLKLRREMQVELKRIQTEVGLTFVHVTHDQEEAMTMADTVAVMNLGKIE
                     QMGAPEVLYDLPRTKFVANFLGQSNLLSGHVDDTDGEHIIVSAAHHKLRLRKSRAAAG
                     SGDIAFGVRPEKTRIHNTDPGASAAGNSIGPGTLLDVSFTGVATQYLVDVPDAGRWAV
                     YEQNLDLDRIDSRPGDTVWISWDPGHAFGVRP"
     gene            7738..8655
                     /locus_tag="PLB86_03340"
     CDS             7738..8655
                     /locus_tag="PLB86_03340"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC64200.1"
                     /translation="MSALAHIGGSPSGTSTTAAPEPTKRGGMAAYLLLLPGLIWLLVF
                     FVAPLVQLATVSLQSQFPGYPGYYYRNVDFGNYGRAIVEYWPHFVRSLLFAGLATFFA
                     FVLAYPLAYAMAFKAGKWRGVMMICVIAPFFTSFILRTIAWRQILSDEGPVVSVLNFL
                     HVLPGGHITETWVAVVAGLTYNFLPFMVLPIYASLERADPKIIEAGGDLYANGFTTFR
                     TVTLPMSMPGVLAGTLLTFIPAAGDYVNAELLGSDISTKMVGNGIESQFFKVLGGYPI
                     AAALSFTLMAVILALVFLYVRRYGTEELL"
     gene            8700..9479
                     /locus_tag="PLB86_03345"
     CDS             8700..9479
                     /locus_tag="PLB86_03345"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC64201.1"
                     /translation="MIVALLVLFYLFLPVLYTFVFSFNNYKKSNISWNPEGSPTLEHW
                     QNPCGPPGLCESLGTSIKVGGIATLVATVLGTMLAFALVRHRFKGRGAGNILVFIPMA
                     TPEIVLGASLLTLFVQGFGQYGLKLGFWTIVFAHIMFCISFVVVTVKARLQSLDPRLE
                     EAAQDLYAGPGSTFWRVTFPLVLPGIVSAALLSFSLSFDDFIITNFVSGDTSTFPKFV
                     YVSYLRGIPAQANVIGFSMFVIAVLLVVVGQVIGSRRRSPA"
     gene            9493..10749
                     /locus_tag="PLB86_03350"
     CDS             9493..10749
                     /locus_tag="PLB86_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRC64202.1"
                     /translation="MRILMIGAGGVGDAAARIAVERDFFEALVVADYDLGRARATVAA
                     ASARRPGESRLVAAYVDASDAESVARLAREHQITHVFNAVDPRFVMSIFAGARAAGAN
                     YLDMAMSLSRPHETRPHELTGVKLGDEQFAQAGEWEAEGRLALVGIGVEPGLSDVFAR
                     YAADHLFTTIDELGTRDGANLVIRDETGAEVFAPGFSMWTIIEECLNPPVVWEKDRGW
                     FTLAPFSEPEVFDFPEGIGPVECVHVEHEEVLLMPRWVDAKKVTFKYGLGEQMITILR
                     TLHALGLDRTDPVRVKGVEVSPRDVVAACLPDPATIGPRMTGKTCAGVWVTGTGRDGA
                     PRATYLYHVVDNEWSMREYGAQCVVWQTAINPIIALELLATGVWLGAGVRGPEAFDAV
                     PFLDLLSTPKPAGYGSPWAIQDRTPA"
     gene            10773..11807
                     /gene="speB"
                     /locus_tag="PLB86_03355"
     CDS             10773..11807
                     /gene="speB"
                     /locus_tag="PLB86_03355"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRC64203.1"
                     /translation="MSRYGAQFGPDVTFLGVPPCDLEDESTYAGADIVILGAPFDGGT
                     SHRPGTRFGPMAIRTTDYLAHDGSRPSLALRVDGLQDLRVVDAGDVELPPGDIETAMA
                     RIEAAVERIARSGAIPLILGGDHSIAFPDAKGVANVLGHGRISMIHFDAHADTGDIEF
                     GSLWGHGQPMRRLIESGALRGDRFLQVGLRGYWPGPETLDWMAAQRMRSYEMTEIGHR
                     GLATCLTEAFTIATDECDGVFLSVDIDVCDPGHAPGTGTPEPGGLSARELLDSVRRIC
                     LELPVVGIDVVEVSPPYDHADITAALANRVVLEAISAIARKRRDAAYGTSWNPATPLL
                     ADRHQPRQEP"
     gene            11811..13151
                     /gene="gabT"
                     /locus_tag="PLB86_03360"
     CDS             11811..13151
                     /gene="gabT"
                     /locus_tag="PLB86_03360"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HRC64204.1"
                     /translation="MTTPIEQRRILRTAIPGPASAALHERKLASVSAGVSTGLPAYVV
                     AGQGGILVDADGNQLIDFGSGIAVTSVGNGNQRVIDAVSKQIAEFTHTCFMVNPYEQY
                     VAVAEKLNELTPGSHEKRTALFNSGAEAVENAVKVARHFTGKDGIVAFDHAYHGRTNL
                     TLAMTAKNMPYKHRFGPFASELYRVPMAYPYRWPGGPQHCADEAFNYIVDQIHAQIGE
                     ENTAAVIIEPIQGEGGFIVPPAGFLKELAQWCAANNILFIADEVQSGFCRSGDWFASD
                     YEGIVPDLITTAKGIAGGMPLSGLTGRADIMDTIHPGGLGGTYGGNPVACAAALASIE
                     TMEELNLAARAREIEAILRADLEKLAATYPQVGEIRGRGAMIAMELVTDRETKEPAAA
                     LTAAINKACHAQGLVTLTCGTLGNVFRFLPPLVISDDLLHEGMSILADAFAASV"
     gene            13190..14650
                     /locus_tag="PLB86_03365"
     CDS             13190..14650
                     /locus_tag="PLB86_03365"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRC64205.1"
                     /translation="MAMTQGALLESVPKGLFIDGAWRDASDGATLPVDDPATGKILAH
                     VASATVADGKAALDAAVAAQRDWARTAPRDRGEILRRAYEAIIARTDDFALLMTLEMG
                     KPLAEARGEVVYGAEFFRWFSEEAVRIHGRYAVAPNGATRLMTMKQPIGPTLMITPWN
                     FPLAMGTRKIGPAIAAGCTMVVKPAAQTPLTMLLLTQVLIECGVPAGVLNVVSTAHTG
                     EVMEPIIRDPRLRKLTFTGSTQVGQRLVEQSAQGLLRLSMELGGNAPFIVFDDADLDR
                     AVDGAMLAKMRNIGEACTAANRFLIHESVAEEFGRRFAERMAALRVGKGTVKGVDVGP
                     LVDRKARESVDALVQEAVAGGAKILTGGGPLPGKGWFYAPTVLTDVPASSRIAVEEIF
                     GPVAPISTFATEAEAIARANDTAYGLVAYAFTRDLARALRVSEELEFGMVGINQGVVS
                     NPAAPFGGMKASGFGREGGFEGIDEYLEVKYVGIAP"
     gene            14753..15781
                     /locus_tag="PLB86_03370"
     CDS             14753..15781
                     /locus_tag="PLB86_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRC64206.1"
                     /translation="MSQQGGRSGARHRGITSLAELLASADGRMQDGARIGASIWPTGL
                     EPLDGHLGGGLRGGELTLIGGPQGLGKTTMALQMVRNTVAAGRSAIVFSFEHEGRILL
                     ERLIALEAATVAGRGSVTIRQLRDVFEEGSPHGGGLIERLAQLPGAAEGIAAQEAYAA
                     RLHLHESSGSTTDMDEIREIVAQLKEAGEEPLVLVDYLQKVPILGGRHVEEERVTEVV
                     EGLKELALDADLPVVAIVAADKAALVAGHRMRLHDLRGSSALAYEADIALILNGKYDV
                     VARHHLMFDVANAERFKNWVVVTVEKNRSGLADISMEFEKRFHEGRFDPKGRMVQEQL
                     TEGRVFLE"
     gene            15842..16312
                     /locus_tag="PLB86_03375"
     CDS             15842..16312
                     /locus_tag="PLB86_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HRC64207.1"
                     /translation="MSRSTRQLLQLVAVGVVAIVLVAAYLLARGGSAGTAGVGAQPTA
                     RPTTSTQATGGTVDPASGLVWVDESTLPAQAKATLALIRAGGPYPYPRNDDQTFGNRE
                     GLLPKKSGGYYKEYTVPTPGSDDRGERRIIKGAGGELYYTSDHYQSFRRIREGR"
     gene            16315..16653
                     /locus_tag="PLB86_03380"
     CDS             16315..16653
                     /locus_tag="PLB86_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HRC64208.1"
                     /translation="MLGRRADLHAVIAQAQAAGRSVRLVVGGMDKQSVLTVFADAIGA
                     PDWFGNNYDALTDALREVSDPDGRPIEIIWDGVAVLRSLDKPTYVAVRELLREVDEER
                     SDLTVTIVDR"
     gene            16795..17934
                     /locus_tag="PLB86_03385"
     CDS             16795..17934
                     /locus_tag="PLB86_03385"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HRC64209.1"
                     /translation="MTYQPGDVANGYVLGEDYQWHPLPPAETAPAAPVEPAPTSDPVA
                     PPQWVAPADPITSSDPVASTGPIAASDPTAPAYSPVPYSPVGEPTSAPPALPTPPTTA
                     LPQTAPQGYPQEPAPTYAPQPYQQPGYPGQGYPQQGYQQPGYPQQGHPQQPGYPQQGY
                     AGATPPGPPTKRPFYKKWWFWLIAALLVIAVVVAVVIAALRTFGNAITSVTTVTSTAQ
                     ATPRPTTSSPKPSTTTPQSSTTTTTTPPPATGGSGQPGRDGDLQFTVTGVDCTKNSVG
                     TAPLTMKADGVFCLVSLTIDNSGTKDADLNPFFQEATDSKGDVHFPDLAALVFMDDAD
                     SFTDPVPAGGTAKGILPFDVPAGSTLTSIELHESFNSPGTKIAIP"
     gene            17953..19200
                     /gene="dxr"
                     /locus_tag="PLB86_03390"
     CDS             17953..19200
                     /gene="dxr"
                     /locus_tag="PLB86_03390"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRC64210.1"
                     /translation="MSSDLGPCRTVTLLGSTGSIGTQAITVIQANPQRYRVVGLSAGG
                     SDLRQIAAQAVEQQAEVVAVARGAIDDVRAAIAAAAAEAGDPAYQPEVLVGPDASREL
                     AARPVDVVLNGMTGAIGLEPTLAALRAGSTLALANKESLIIGGPLVKALAAPGQLVPV
                     DSEHSAIAQCLRGGRASEVRRLVLTASGGPFRGMPRADLHDVTPEQALAHPNFSMGRV
                     VTTNSATLVNKGLEVIEAHLLFDVPFERIAVVVHPQQQIHSMVEFQDGSTMAQVGPPR
                     MLVPIALGLSWPDRLPNVDVPTDWSVAQSWQFLPLDDEAFPAVALARRAGSTGGTGPA
                     VYNAANEVCVDAFHDGAIRFTDIVDTVARVLDGHLAGDAQEVCRAGTGSDSPDLDVAG
                     VLRADQWARGEARRVLGLSEEQL"
     gene            19197..20546
                     /locus_tag="PLB86_03395"
     CDS             19197..20546
                     /locus_tag="PLB86_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRC64211.1"
                     /translation="MTTVYYIAGILLIVLGVGVSIALHELGHLVPAKRFGVKCSQYMI
                     GFGPTLWSRTIGDTQVGVKAIPLGGYVRMIGMIPPRAGDAPGQLRSMSTSRMGTLVDQ
                     ARQQSMDEIEPGDENRVFYKLSVPKKVTVMLGGPLMNLALAFVMLTILMSGIGVQSLV
                     PTVSTVAQCIPQTPPTVAKPFADCVAGDPKAPSVAAGLQAGDTIVEVGGVPVQLWAEA
                     TAKIRASAGQPLDLMVERTGTRVPLTVQVGQVVRPTFTEEGKVAVRADGTVITETVGY
                     LGASASVGYVRQPLSAVPGELGRMLGATASVLLRIPEKMVGVFEAVTGQSDRDPNGPV
                     SVVGVARAGGEIVSGQYGPETGQSIAYRIIGLIFGLNLALFLFNLVPLLPLDGGQVVG
                     ALWEGVKKTWARLRGRPDPGYVDVAKALPIAYAVSTVLIAMTLLLAYADLVTPIKLG"
     gene            complement(20622..21260)
                     /locus_tag="PLB86_03400"
     CDS             complement(20622..21260)
                     /locus_tag="PLB86_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HRC64212.1"
                     /translation="MTRYAQLERHALADTMLAVGPDAPTLCDPWRVRDLAAHLIIRET
                     RPDLAAGMFVPRLQARLERAQDELAATDFGELVERVRSGPPRWSAAGVPAVDEAMNRM
                     EFFVHHEDVLRGDPTTPRRDLDPGLAKAVWSGLIRTGGLLYRRAPVGVILVADGVGRQ
                     VVRRPSDVGSAVVRGSVAELVMYSYGRKRAADVKITGPQAAVAALTATDIAL"
     gene            21305..22342
                     /locus_tag="PLB86_03405"
     CDS             21305..22342
                     /locus_tag="PLB86_03405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC64213.1"
                     /translation="MIELRRPDASLHREWLDFVSDYDRPGVDGGSVRDDELAGLADPG
                     VFAAWVAQLHDQEAGRNIAPDRVPCTSRWIVREGEIVGVINLRHELNDLLLAWGGHIG
                     YAVRTSVRRQGIATRALALMLGEAARLGINPVLVTCDEDNVGSRRTIEGAGGVYDGSV
                     EGKRRNWITLADTPIGYAVRPLSLDPLRGRLVELRVMSNDEVRALHSGERRPDWAAEF
                     PRPDDLDGSPLRDVEALKGREWVSRMIVRRSDHQVVGTIGCFGPPDQADTTSTVEIGY
                     GMVESARGHGLITDALRLLVPAASARGVTLQAHTAYDNRASCRALERAGFTPSGEADD
                     AGRLRFVHPRD"
     gene            22352..23332
                     /locus_tag="PLB86_03410"
     CDS             22352..23332
                     /locus_tag="PLB86_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC64214.1"
                     /translation="MSILEARDLRRTFTTTTGVLRRNRKQVEAVRGVSFAIEPGELFG
                     LLGPNGAGKTTTIKMLITLLLPTAGSANILGLDVVRDAREVRRHIGYVFGGDRGLYER
                     LSAQDNLRYFAELYAVPARDQRARIGELLELVGLTGREKERVEGFSRGMRQRLHIARG
                     LLHDPALLFLDEPSIGIDPVGARELRATIAGLRSAGKTILLTTHYMFEADELCDRIAV
                     LSQGELVAEGTPAQLKARVTAGSVVEVETFSGVGPADVRGKVDAVGALPGVRSVVVDD
                     RPDGETVVVQVEPGVDVTAAVLGTLTGIRVGRVVTREPTLEDAYVQLVSS"
BASE COUNT         3736 a         8129 c         7914 g         3703 t
ORIGIN      
        1 tcttcggtgg ggcctgaccg accctgggct gccggtcgcg cccgaattcc ccacactcgc
       61 cgatgatctg gggaggtccc ggccctggct gccccaagtt ccccacactc gccggtgatc
      121 tggggaaggg gtatgccggt cgcgcccgaa ttccccacac tcgccggtgt tgtagcgcgg
      181 ggcttcgcca ttcggccgct caggtgagcg agaccggcgc cctggcgtcg gtttcgctca
      241 cccgaacggg gtgccatggg ccgacaggct gaccggtttg gtcagggttg gtcagggttg
      301 gtcagggtga ctacctcagt ggccggtgag ggcggacaac acccgtccca gcaccgcctc
      361 gcggcccgtg agtgactcct cgagatccgc ccaccccgcc actcgcagcc cggcgttgac
      421 gcccagccac cgcaccggct ccggctccca cgacggcgag acatggccca cccacggcag
      481 ccgggtcagc ccgcttgccc gccccagcac cagatcggcc agcgtccgcc cggccaagtt
      541 gctcgccgcc acaccatcgc ccacataacc gccggcccac cccaagcgcg ccacggggtc
      601 gtagcccacc gacggatgcc agtcccgcgc gatccccagc ggtccgcccc aacggtgggt
      661 cacggcatac ccctgcagga cggggaacag cccacgcaag gtgctctcca actccgcgaa
      721 aaccgttggt tccgaatcga actcgggccg aatctcggac ccccagtgat agggcgcccc
      781 ccggccgccg aaggcgatcc ggtcatcagc cgtccgctgg ccatagatga tgacgtgccg
      841 gtggtcggcg aaggtctcgc gctgggccag cccgatctcg tcccacacac cggcgggcag
      901 cggctcggta gcgatcatca aggagtagac cggggtcgtg cgccgtcgca gccccgccag
      961 ccgcgcggtc caagcctcgg tcgcccgcac ggtatgccgg gtccgcacca ccgtccccga
     1021 ctccaggacc actcgaccgg gcgacgcgga cgtgacccgg gcaccctcga caatcagccc
     1081 gcctcgcgaa cgcaccactg ccgcaagccc gtccacaagt ttgcgcgggt ggatccgcgc
     1141 gcagttgggg ttgtaggtcg cgcccaacaa ccccgacatc cgcacccggt ctcgggcctc
     1201 cggcaggtca agccagacgg tgccatcgcc ccaggccgcg ccggcagcgg catacccgcg
     1261 ggctcgctcg acctgcgcgg gagtccgcgc ggcgagcacg acgccaccac gtcggtagtc
     1321 gcagtcgatg ccttcgtcgg cggcgacccg gccgacttcg aggacggtct cacgcagggc
     1381 cgcgcccatg gatcgcgctg cggcatcacc tgattcgcgc gccaaggtgc tggccgacac
     1441 cggccacaac gccgacaccc agccaccgtt gcgcccgctc gccccgaacc cgacgtgctc
     1501 ggcctccagc acgacgacgg acaacgacgg gtccagcccg agcaggtagt acgccgtcca
     1561 caggcccgtg aagccgccgc cgacgatggc gacgtcgacc tcggacgggg gctgcgcggc
     1621 ataccccgcg cccacagggc ccaccccggg tgccgcggca ggggagggta tgccgccctc
     1681 ccaccacagc gggcgggtca caggtgcgct tcggcgccct gcaggaccga gcgcagccga
     1741 gaggtgatgt cgtcgaactc aggctggccg atgatgagcg ggggagcgag ttggatgacg
     1801 ggatcgcccc ggtcgtcggc ccgacagtag atgccggcgt cgaagagggc cttggacagg
     1861 tagccgcgca acaaccgctc gcactcgccg tcgtcgaagg tctcgcgggt ggctttgtcc
     1921 ttgaccaact cgatgccgta gaagaagcct tcgccgcgga catcgccgac gatcggcagg
     1981 tcgagcaggg tctccaggga ggcgcggaag gccggggccg tctcgcggac gtgggcgttg
     2041 agcccttcct tctcgaagat gtcgaggttg gccatggcga ccgcgcacga gatggggtgg
     2101 ccaccgaagg tgtagccatg cgggaagtag gtctgcccgt gccggaacgg ctcgaacagg
     2161 cggtcgctga cgatcatgca tcccagcggt gcatagccgg acgtgacgcc cttggccgtg
     2221 gtgatgatgt ctgggacgta gccgagttgg ttgcaggcga agtagtcgcc gatccgaccg
     2281 aaggcgcaga tcgtctcgtc ggagacgagc agcacgtcgt actcgtcgca gatctcgcgg
     2341 acccgctcga agtagccggg gggcggaggg aaacaccccc cggagttctg caccggctcg
     2401 aggaagacgg cggcgacggt ctcgggccct tcgaactcga tggcctcggc gatccggttc
     2461 gcggcccaca gcccgaacgc cttcgggtca tcgcgcaggt gttccggcgc gcgatagagg
     2521 ttggtgttgg gcaccttgtg gccgccgggc acgagcggct cgaagaccgc tttggcggct
     2581 ggaatgccgg tgatcgacaa ggccccctga ggagtgccgt gataagcgat gttgcggctg
     2641 atcaccttgg tcttcatcgg cttgccgacg agcttgtagt actgcttggc cagcttccag
     2701 gccgactcga cggcctcgcc gccgccggtc gtgaagaaca cccggttgag gtcgccaggg
     2761 gcgtagtgag caaggcgctc ggccaactcg acggcccgcg ggtgggcata ggaccacagc
     2821 gggaagtagg cgatcttctg gaactgtgcc atggcggcgt cggccaactc ctgccggccg
     2881 tggccgacct ggacggtgaa caggcccgac agtccgtcga ggtatttgtt ccctcggtcg
     2941 tcccagatgt aggccccgtc acccttgacg atgagcggca cggtgtgggc gccgtcgcga
     3001 tcctcgaaga ccgaatggcg ggtgaagtgc atccacaggt gatcccgggc ggcctccgaa
     3061 tagagcttgc cggtgccggt gtggaagtcg gagctgggtt cccagatctc ggacgggtag
     3121 atcgtcatcg tgtgccccag ttgtagtgct gcttgttgag ttcgagatag acgaacgtct
     3181 ccgtggctcc cacacccggc aacgtccgga tccgctgcga gatgacctcg agcaggtggg
     3241 cgtcgtcctc gcacaccacc tcggcgagca ggtcgaagct tccggccgtc gtcacgacgt
     3301 agtcgacctc ggggatcgcg ctgaccgctt cggcgaccgg gccgatgtcg ccctggacct
     3361 tgatcccgac catcgcctga cggaggaagc cgacatgcag ggggtcggtg acggcgacga
     3421 tctgcacgac cccgccgtcg acgagtttct gcacccgttg tcggacggcc gcctcggaca
     3481 gccccactgc ggtcgcgatc gcggcatacg accgacgtcc gtcctcttga agttgctcga
     3541 tgatcgcctt cgcggtggcg tcgagaactt gccgggtcgg gcggcgggtc gaacgtctca
     3601 tggacggtcc cgatgtcatg gggtctgatc gtgcccgctg cggtgccggt tggcaaggcc
     3661 cggccgggct cgacccttcg gaatctatcg ttttcgggtc cagggagcgc agaatgcaaa
     3721 gtttctatgc cttgccacta ccgaaaacgg tcgccagcgg gctagattgc ccgaaaccgc
     3781 gtccggaccg ggagccggcg gccagctcaa cggaggtctc tatgtctcaa gccccacgcg
     3841 tgttgcgcaa ccacgtcggc ggcgagttcg tcgacgcgac ggccgatgcg cgcagcgatg
     3901 tcgtcaaccc ggcgaccggc caggtcgtcg ccacggcccc cgtctccggg caagaggacg
     3961 tcaaccgggc gtatgccgcc gcttccgcgg ccttcgagga gtggggccgc acgacaccgc
     4021 gcgagcggca ggaggccctg ctgaagttcg ccgctgcggt ggaggctcgg gccgacgact
     4081 tcgtccacct tgagggcgag aacaccggca agccgcacgc gctcaccgcg agcgaggagg
     4141 tgccgccgat gctcgaccag atccgcttct tcgccggcgc cgcccgcgtt ctggagggac
     4201 gctcggcagg ggagtacatg ccggggcaca cctcgtggat ccgccgcgaa cccatcggcg
     4261 tgatcggcca ggtcaccccc tggaactacc cgatgatgat ggccatctgg aagatcgccc
     4321 ccgccctggc ggcaggcaac acggtcgtcc tcaagccgag tgacacgacc cccgagacca
     4381 ccctgttgct cgctgcgttg gccgccgact gtttccccgc cggcaccttc aacgtcgtca
     4441 ccggggaccg cgagaccggc cggatggtcg tgtcgaaccc ccgggccgac atggtcgcca
     4501 tcaccgggtc ggtgcgggcc ggctcgcagg tcgccgccgc tgccgccgag agcgtcaagc
     4561 gggtccacct cgagctcggc ggcaaggcgc cggtcgtcgt cttcgatgac gccgacatcg
     4621 aggcggccgt cgaggggatc gccatcgcgg gctacttcaa cgcggggcag gactgcacgg
     4681 cggccacccg ggtgctcgcc ggccccggga tctacgacga cttcgtcgcc gccctggggg
     4741 agtatgccgc gaactcggcc aaggtcggca tgcccgacga cgaggacgcg ctcttcgggc
     4801 cggtcaacaa ccccgcgcag ttggagcggg tgacgggctt cctcaaccgg gtgccggccc
     4861 acgcgtctgt ggtggccggc ggctcccgcg tcaccgacct cggcgatggg tatttcatcg
     4921 ccccgacggt gctcgccggc ctgctccagg acgacgaaca catccagaac gagatcttcg
     4981 ggcccgtcat caccgtgcag cgcttctccg atgaggacga ggcgatccgg tgggccaacg
     5041 gcgtcgacta cggcttggcg tcgtcggtct ggacgaagga cttcgggcgg gccatgcgga
     5101 tgtcgcgggc cttggatttc gggtgtgtgt ggatcaacac gcacattccg atcgtggccg
     5161 agatgccaca cgggggatac aagcacagcg gttacggcaa ggacctgtcg atgtacggct
     5221 tggaggacta cacccgtatc aagcacgtca tggccaatat ccaggcctga cgcaatcacc
     5281 gcgccgatcc ctcggccgga accttctcaa cccactcccc ggcgggcctg gctccgccgc
     5341 acgtcgtccc taccgcggcc tcgacggccc ggcattcctc tcaaggaagg agcggtgcga
     5401 tgcgccgatc cgaaccacct cgtgatccgc aggcgcgggc cctggcctcg ctcgcggcaa
     5461 gctctctgtc ccgtcgtcgg ctgctgtccg gcgccggcct cggcctggcc ggtgcgggcc
     5521 tggccgcgtg tgcgccgccg ttgccaccgg ccgcgaccgg cccgaccggc ccgaagctgc
     5581 ccgtcgacaa gtcggccacc gacaagaccg tcgccttcgc caactggacg gcctacctcg
     5641 actacgacga ggaggccaag aactacccga cgttggaggc cttcatcaag gagaccggca
     5701 tccaggcctc ctacacggag gacatcgagg acaacgacgc ctacgtctcc aagatcaagc
     5761 cgcagttgca ggcaaccccg ccgcaggaca tcaaccggga catcttcgcc ctcacggact
     5821 ggatggccaa ccgggtgatc cgcgacggtc tggtgcagcc gctggacctc atccggatgc
     5881 cgcacgccgc caacctgctc gactccctca aggacgtgtc cttcgacccg ggccgcaagt
     5941 actcactgac ctggcagagc ggtttcgccg gaatcggcta cgacaagagc aaggttggcc
     6001 gcgagctcaa gagcctgtcg gacctgtggg ccgacgatct caagggcaag gtcgtggccc
     6061 tctcagagat gcgcgacacc ctcggcctga tcatgcagtc gcaaggcgtc gacatctccg
     6121 gctcgttcac caaggaccag gtctccaagg ccatggacga ggtgtccaag cggatcgccg
     6181 atcagaacat ccgccggatc aagggcaact cctacatgga ggacctcaag agcggcaacg
     6241 ccatcgccgg catcgtctgg agcggtgacc tgttcatcct gcgggccgag accgagaacg
     6301 agaactggga gttcgtcatc cccgactccg gcggcacgct ctggagcgac aacatgatga
     6361 tccccatcac ctcgacccat cgccgcaacg cgatggcgct gatggactac tactaccgcc
     6421 ccgacgtggc agcccaggtc gcggcctacg tcaactacgt gtgcccggtc gtcggagccc
     6481 aggcggagtt ggccaagacc gaccccgagc tcgccgagag cccgttcatc ttccccactt
     6541 ccgagtggat cgcagaacac aagatccagg ggttccgggc gctggacgcc caagaggacg
     6601 cggactactc cgcgatgtgg gcaaaggtgg tgggcaactg atggccgagt tccgcgcagc
     6661 caagggcgac ctcgtcctgg agaacgtcac gaagagcttc accaacttca ccgccgtcga
     6721 tgacctgtcg ctgaccgtgc cccggggggc gttcttcgcc ctgctcggcc cgtccgggtg
     6781 cggcaagacg acgacgctgc ggatggtcgc cggcctcgaa cagccgacca gcggccgggt
     6841 gctcatcggg gacaccgacc tcacggggtc ccgtccctac gagcggccgg tcaacaccgt
     6901 cttccagtcc tacgccctct tcccgcacct caccgtccgg gacaacgtcg ccttcggtcc
     6961 gaagcggcgg ggagccaagg acgcccaggc ccaggccgat gcgactctcg cgctggtcca
     7021 actcacccac ctcgccgggc gcaagccgac ccagctctcg ggcggtcagc agcagcgagt
     7081 tgccgtcgcc cgggcactgg tcaaccaccc cgaggtgctg ctgctcgacg agccgctcgg
     7141 tgccctcgac ctcaagctgc gtcgcgagat gcaggtcgag ctcaagcgga tccagaccga
     7201 ggtcggtctc accttcgtcc acgtcaccca cgaccaggag gaggccatga ccatggccga
     7261 cacggtcgcg gtgatgaacc tcggcaagat cgagcagatg ggtgcgcccg aggtcctcta
     7321 cgacctgccg cggaccaagt tcgtcgccaa cttcctgggt cagtccaacc tgctctccgg
     7381 gcacgtcgac gacaccgatg gcgagcacat catcgtgtct gccgctcacc acaagttgcg
     7441 gttgcgcaag agccgggcag cggcgggcag cggcgacatc gccttcgggg tccgccccga
     7501 gaagacacgg atccacaaca ccgacccggg cgcgagcgca gccggaaaca gcatcggccc
     7561 cggcaccctg ctggacgtgt ccttcaccgg cgtcgccacg cagtacctcg tcgacgtccc
     7621 cgacgccggg cgttgggcgg tctacgagca gaacctcgac ctggaccgga tcgacagccg
     7681 cccgggcgac acggtgtgga tcagctggga ccccgggcac gccttcgggg tcaggccatg
     7741 agcgccctcg cgcacatcgg cgggagcccc agcgggacgt cgaccacggc ggcgccggaa
     7801 cccaccaagc gaggggggat ggcggcatac ctgctgctgt tgccgggcct catctggctg
     7861 ttggtcttct tcgtcgcccc gctcgtccaa ctggcgaccg tgtcgttgca gtcgcagttc
     7921 ccgggatatc cgggctacta ctaccgcaac gtcgacttcg ggaactacgg ccgggccatt
     7981 gtcgagtatt ggccgcactt cgtgcggtcg ctgctgttcg ccggcctcgc gacgttcttc
     8041 gcgttcgtcc tggcctaccc gttggcctat gccatggcat tcaaggcggg caagtggcgt
     8101 ggagtgatga tgatctgcgt catcgcgccg ttcttcacgt ccttcatcct gcgcaccatc
     8161 gcctggcgcc agatcctctc cgacgagggc ccggtcgtgt ccgtgctcaa cttcctccat
     8221 gtcctgccgg gcgggcacat caccgagacc tgggtggcgg tggtcgcggg tctgacctac
     8281 aacttcctgc cgttcatggt cctgccgatc tacgcctcgc tggagcgcgc cgatcccaag
     8341 atcatcgagg ccggtggcga cctctatgcc aacggcttca cgacgttccg gaccgtgacc
     8401 ctgccgatgt cgatgccggg tgtgctcgct ggcactctgc tcaccttcat cccggcggcc
     8461 ggtgactacg tcaacgccga actcctggga tcagacatct ccacaaagat ggtcggcaac
     8521 ggcatcgaga gccagttctt caaggtgctc ggtggctatc ccatcgcggc ggccctgtcc
     8581 ttcaccctga tggccgtcat cctcgcgctc gtgttcctct acgtgcgccg ctacgggacg
     8641 gaggaactgc tgtgagcacg ggtcagcggc tgaagttctg gttcgccgaa cgcttcgcga
     8701 tgatcgtcgc gttgctcgtc ctcttctatc tcttccttcc ggtgctttac acgttcgtct
     8761 tctcgttcaa caactacaag aagtcgaaca tctcctggaa ccccgagggg tcaccgaccc
     8821 tcgagcactg gcagaacccg tgtggtcctc ccggactgtg cgagtcgttg ggcacgtcga
     8881 tcaaggtggg tgggatcgcc accctcgtgg ccaccgtgct cggcacgatg ctcgccttcg
     8941 ctctcgtgcg ccatcgcttc aaggggcgtg gagccgggaa catcctcgtc ttcatcccga
     9001 tggcgacgcc ggagatcgtc ctgggtgctt cactgctcac cctcttcgtc caggggttcg
     9061 gccagtacgg cctcaagctc gggttctgga cgatcgtctt cgcccacatc atgttctgca
     9121 tcagcttcgt cgtcgtcacc gtcaaggcgc ggctgcagag cctcgaccct cgcttggagg
     9181 aggctgcgca ggatctgtat gccggtccgg gcagcacctt ctggcgggtc accttcccgc
     9241 tcgtgcttcc gggcatcgtg tcggcggcgt tgttgtcgtt ctcgttgtcg ttcgacgact
     9301 tcatcatcac caacttcgtc tcgggcgaca cgtcgacctt cccgaagttc gtgtacgtct
     9361 cctacctgcg tggcatcccg gcccaggcca acgtcatcgg tttctcgatg ttcgtcatcg
     9421 ccgtcctgct ggtcgtcgtg ggtcaggtca tcggctctcg tcggcgctcg ccggcctagg
     9481 agtagatagc tcgtgcggat cctcatgatc ggggccggcg gggtcggaga cgccgccgcc
     9541 cggatcgctg tcgaacggga cttcttcgag gcgctcgtcg tcgcggacta tgacctgggc
     9601 cgagcccggg ccacggtcgc ggcggcgagt gcccgaaggc ccggcgagag tcggttggtg
     9661 gcggcatacg tcgatgcctc cgatgccgag tcggtggcga ggctggcgcg cgagcaccag
     9721 atcacccacg tcttcaacgc cgtggacccg cgcttcgtca tgtcgatctt cgccggcgcg
     9781 cgcgcggcag gagcgaacta ccttgacatg gcgatgtcgt tgtcgcgacc gcacgagacc
     9841 cgcccgcacg agttgaccgg cgtcaaactc ggcgacgagc agtttgcgca agcgggggag
     9901 tgggaggccg agggccggct cgcgttggtg gggatcggcg tcgaaccggg gctgtcggac
     9961 gtgttcgcga ggtatgccgc cgaccacctg ttcacgacga tcgacgaact cggcacgcgc
    10021 gatggtgcca acctcgtcat ccgggacgag accggcgcgg aggtcttcgc gccgggcttc
    10081 tcgatgtgga cgatcatcga ggaatgtctc aacccgccgg tggtctggga gaaggaccgt
    10141 ggctggttca ccctcgcgcc cttcagtgag ccggaagtct tcgacttccc cgaaggcatc
    10201 gggccggtcg agtgtgttca cgtcgagcat gaagaagtac tgctcatgcc ccgctgggtc
    10261 gatgccaaga aggtcacctt caagtacggc ctgggcgagc agatgatcac catcctgcgc
    10321 accttgcacg ccctcggcct cgaccgcacc gatccggtcc gggtcaaggg ggtcgaggtg
    10381 tcgccgcgcg acgtcgtggc cgcatgtttg cccgacccgg ccacgatcgg gccgcggatg
    10441 accggcaaga cctgcgccgg ggtctgggtg acgggcaccg gaagggacgg tgcgcctcgg
    10501 gccacctacc tctaccacgt ggtcgacaac gagtggtcga tgcgcgaata cggagcccag
    10561 tgcgtcgtct ggcagaccgc aatcaacccc atcatcgcgc tggaactgct cgccaccgga
    10621 gtgtggttgg gcgcgggtgt gcgtggtccg gaggcgttcg acgcggtgcc gttcctcgac
    10681 ctgttgtcca ctccgaagcc agcgggatac ggctcgccgt gggcgatcca agaccgaacg
    10741 ccggcttgac tcactctcct aggattccca ccatgagccg ctacggcgcg caattcgggc
    10801 cggacgtcac cttcctcggc gtccccccgt gcgacctcga ggacgagagc acgtatgccg
    10861 gtgccgacat cgtcatcctc ggcgcgcctt tcgacggggg cacctcgcac cggccgggaa
    10921 cccggttcgg ccccatggcc attcgcacca ccgactacct cgcccacgac gggtctcggc
    10981 cctcgctcgc gctgcgggtc gacgggttgc aggacctgcg tgtcgtcgac gctggcgatg
    11041 tcgaactgcc gcccggagac atcgagacag cgatggcgcg gatcgaggcg gccgtcgaac
    11101 ggatcgcccg ctccggggcc atcccactca tcctcggtgg cgaccactcg atcgcctttc
    11161 cggatgccaa gggtgtcgcg aacgtcttgg gccacggccg gatctcgatg atccacttcg
    11221 atgcccacgc cgacaccggt gacatcgagt tcgggtcgtt gtggggacac ggccaaccga
    11281 tgcggcgact catcgagtcg ggggcattgc gcggcgaccg cttcctgcag gtcggcctgc
    11341 gcggctactg gccgggacca gagaccctcg actggatggc cgcgcagcgg atgcgctcct
    11401 acgagatgac cgagatcggc catcgggggc tcgcgacctg cctcaccgag gccttcacca
    11461 tcgccaccga tgagtgcgac ggggtgttcc tctccgtcga catcgacgtg tgcgacccgg
    11521 gccacgcgcc cggcacagga accccggagc ctggcggcct cagcgcccgc gaactgctcg
    11581 actcggtccg gaggatctgc ctggaacttc cggttgtggg catcgacgtc gtcgaggtca
    11641 gcccgcccta cgaccacgcc gacatcactg cggcgctggc caatcgcgtt gtcctcgagg
    11701 cgatctccgc gatcgcgcgc aaacgccgcg acgcggcata cgggacctcg tggaacccgg
    11761 ccaccccctt gcttgccgat cgacaccagc cccgacagga gccctagaag atgaccaccc
    11821 ccatcgagca acgccgaatc ctgcgcacgg cgatccccgg accggcctcc gccgcgttgc
    11881 acgagcgcaa gctcgcctcc gtgtcggccg gggtgagcac cgggctgccg gcctatgtcg
    11941 tcgcgggtca gggcggcatc ctcgtggacg ccgacggcaa ccaactcatc gacttcggct
    12001 cgggcatcgc ggtgacctcg gtcggcaacg gcaaccagcg ggtcatcgac gcggtgagca
    12061 agcagatcgc cgagttcacc cacacgtgct tcatggtcaa cccttacgag caatacgtcg
    12121 cggtcgccga gaagctcaac gagctgactc ccggcagcca cgagaagcgc acggcgctgt
    12181 tcaactccgg tgccgaggcc gtcgagaacg ccgtcaaggt ggcgcggcac ttcaccggca
    12241 aggacggcat cgtcgcgttc gaccacgcct accacggccg gaccaacctc accctggcca
    12301 tgaccgccaa gaacatgccc tacaagcacc ggttcgggcc cttcgcgagc gagctctatc
    12361 gggtgccgat ggcctacccc taccgctggc cgggtggccc gcagcactgt gccgacgagg
    12421 ccttcaacta catcgtcgac cagatccacg cccagatcgg cgaggagaac accgcggccg
    12481 tcatcatcga gcccatccag ggcgagggcg gcttcatcgt gccgccggcc ggcttcctca
    12541 aggagctcgc gcagtggtgt gcggccaaca acatcctgtt catcgccgac gaggtccagt
    12601 cgggtttctg ccgcagtggt gactggttcg ccagcgacta cgaagggatc gtccccgacc
    12661 tcatcaccac cgccaagggc atcgccggag gtatgccgtt gtcggggctc accgggcgcg
    12721 cggacatcat ggacaccatt caccccggcg gcctcggcgg cacctacggc ggcaacccgg
    12781 tggcgtgcgc agcggccctc gcctcaatcg agacgatgga ggagctcaac ctggcggcca
    12841 gggcccgcga gatcgaggcg atccttcggg ccgacctgga gaagctcgcg gcgacctacc
    12901 cgcaggtcgg ggagatccgc ggccgtggcg cgatgatcgc gatggagttg gtcaccgacc
    12961 gcgagaccaa ggagcccgcc gcggccctca cggccgccat caacaaggcg tgtcacgccc
    13021 agggcctggt gaccctcacc tgcggcaccc tcggcaacgt cttccggttc ctgccgcctc
    13081 tggtgatcag cgacgacctg ctccacgagg gcatgagcat cctcgccgac gcgttcgccg
    13141 cgtccgtctg accactgcct gaactactgc gacacaagga gatccagcca tggccatgac
    13201 tcagggtgca cttctggaat cggtgcccaa gggcctgttc atcgacgggg cgtggcggga
    13261 tgcgtccgac ggtgcgacgc tgccggtcga tgacccagcc accgggaaga tcctcgcgca
    13321 cgtcgcgagc gcgaccgtgg ccgatggcaa ggcggccctg gacgcggcgg ttgctgccca
    13381 gcgagactgg gcgcgcaccg caccccgcga ccgcggtgag attctccggc gggcctacga
    13441 ggcgatcatc gcgcgcaccg acgacttcgc gctgctcatg accctcgaga tgggcaagcc
    13501 gctcgccgag gcccgcggcg aggtcgtcta cggcgccgag ttcttccgct ggttcagcga
    13561 ggaggcggtc cggatccacg gccggtatgc cgtggccccc aatggcgcga cgcgcctcat
    13621 gacgatgaag cagcccatcg gcccgaccct catgatcacg ccgtggaact tcccgctggc
    13681 catgggaacc cgcaagatcg ggccggccat cgccgccgga tgcaccatgg tcgtcaaacc
    13741 cgccgcccag accccgctga cgatgctcct gctcacccag gtgctcatcg agtgcggggt
    13801 ccccgccggg gttctcaacg tggtgtcgac ggcccacacc ggggaggtga tggagccgat
    13861 catccgcgac ccgcggctgc gcaagctcac cttcaccggg tccacccagg tcgggcagcg
    13921 gctggtcgaa cagtccgcgc aggggttgtt gcggctgtcg atggaactcg gtggcaacgc
    13981 gccgttcatc gtcttcgacg acgccgacct cgaccgggcc gtcgacggcg ccatgctggc
    14041 caagatgcgc aacatcggag aagcctgcac ggctgccaac cgcttcctca tccacgaatc
    14101 ggtcgccgag gagttcggcc ggcggttcgc cgagcggatg gcggcgctgc gggtcggcaa
    14161 gggcaccgtc aagggggtcg acgtcggccc gctggtggac cgcaaggccc gcgagagcgt
    14221 cgatgccctc gtgcaggagg ccgtggccgg cggtgccaag atcctcactg gtggtggacc
    14281 gctgccgggg aagggctggt tctacgcccc gaccgtcctc accgacgtcc ccgcgtcgtc
    14341 ccggatcgcc gtcgaggaga tcttcggacc ggtcgcgccg atctcaacct tcgcgaccga
    14401 ggcggaggcg atcgcgcgag ccaacgacac ggcatacggg ctggttgcct atgccttcac
    14461 ccgcgatctg gcgcgcgcgc tgcgcgtcag cgaggagttg gagttcggga tggtcggcat
    14521 caaccaaggt gtcgtgtcca atccggcggc gcccttcggc ggcatgaagg ccagcgggtt
    14581 tggccgcgag ggcggcttcg agggcatcga tgaatacctc gaggtcaaat acgtcggcat
    14641 tgccccgtaa cgaggcggtc ccggggtcgg atcggggccc agcatcgtcc gaacggctcg
    14701 acttggcccc cgagtgatga ccgcagcctt cggacttggt cgaatggagc gtatgagtca
    14761 gcaagggggt cgctcaggcg cgcgacaccg ggggatcacg agcctggccg aactgctggc
    14821 atctgccgat ggtcggatgc aggacggtgc gcggatcgga gcctcgatct ggcccaccgg
    14881 tttggaacca ctcgacggcc accttggggg aggtctgcgc ggcggtgaac ttacgctcat
    14941 cggcggacca caggggctcg ggaagacgac gatggcgctg cagatggtgc gcaacactgt
    15001 tgcggccggg cgcagcgcca tcgtgttctc cttcgaacac gaaggccgca tcctgcttga
    15061 gcggctcatc gccctggaag cggccacggt cgccggtcgg ggcagcgtca cgatccgcca
    15121 actgcgggat gtcttcgagg aagggtcgcc gcacgggggc ggcctcatcg agcggctcgc
    15181 gcagctacct ggagcggcgg agggcatcgc cgcccaggag gcgtatgccg cgcgcctgca
    15241 cctgcacgag tccagcggca gcacgaccga catggacgag atccgggaga tcgtcgctca
    15301 gctcaaggag gccggggagg aaccgctcgt cctcgtcgac tacctgcaga aagtgcccat
    15361 cctgggtggg aggcacgtcg aggaggaacg agtcaccgag gtcgtcgagg gtctcaagga
    15421 actcgctctg gacgccgatc tgcccgtcgt cgccatcgtc gcggcggaca aagcggcgct
    15481 cgtggccggc caccggatgc gcctgcacga cctgcgcggg tcgtcggcgc tggcctatga
    15541 ggccgacatc gccctcatcc tcaacggcaa gtacgacgtg gtggctcgtc atcatctgat
    15601 gttcgacgtg gctaatgccg aacggttcaa gaactgggtc gtcgtcaccg tcgagaagaa
    15661 ccgcagcggc ctggccgaca tcagcatgga gttcgagaag cggttccacg aaggtcggtt
    15721 cgatcccaag gggcgcatgg tccaggagca gctcaccgag ggccgcgtct tcctcgagtg
    15781 agccacgtct tcctcgagtg agctgtcggc tcgcggcaca atgggcgctg tgacctcgcc
    15841 gatgagcaga tccacccgcc aactgctcca actcgtggct gtgggggtcg tcgcgatcgt
    15901 cctcgtggcg gcatacctgc tggcgcgtgg tgggagcgct gggaccgccg gcgtgggggc
    15961 gcagcccacg gcccgcccca cgacctcgac gcaggcgacc ggaggcacgg tcgatccggc
    16021 cagcgggttg gtctgggtgg acgagtcgac gctgccagcc caagccaaag cgacgctggc
    16081 actgatccgc gcaggcggac cgtatcccta tccccgcaac gacgaccaga ccttcggcaa
    16141 ccgcgagggg ctgctgccga agaagtccgg cggctactac aaggagtaca ccgtcccgac
    16201 ccccggctcc gatgatcgcg gagagcggag gatcatcaag ggcgcgggcg gcgagttgta
    16261 ctacacctcc gaccactacc agagtttccg acggatccgg gagggtcgat gaggatgttg
    16321 ggccgcaggg ccgacctgca tgcggtgatc gcgcaggcgc aggccgccgg gcgcagtgtc
    16381 cgactggtcg tcggcggcat ggacaaacaa tcggtgctca ccgtcttcgc ggacgccatc
    16441 ggggcgccgg actggttcgg caacaactac gacgcgctca ccgacgctct gcgcgaggtc
    16501 tccgatccgg acggacgacc gatcgagatc atctgggacg gggtggccgt gctgcgcagt
    16561 ctcgacaaac ccacctacgt ggccgtccgc gaactgctcc gagaggtcga cgaggagcgc
    16621 tctgacctca cggtgacgat cgtcgatcgc tgacctgtgg cgctgtcgag ccgcccgccc
    16681 gaggtgatcg cccggggccg gacgcccgcg ccgattccga ggtcggacga taggctggca
    16741 gggacggccc ggggtccccc gcgtgcctga gtcggcacgc tgaagggcac gcgaatgacc
    16801 taccaaccgg gcgacgtcgc caacggatac gtcctgggcg aggactacca gtggcatccg
    16861 ctgccgcctg ccgagacggc tcccgccgcg ccggtcgagc ccgccccgac gagcgacccg
    16921 gtcgcgccgc cccagtgggt cgcgcccgcg gacccgatca cctcgagtga cccggtcgcc
    16981 tcgaccggcc ccatcgcagc cagcgacccc actgcgccgg cctattcgcc cgtgccgtac
    17041 agcccggtgg gggagccgac ctcagccccg ccggccctgc cgaccccgcc caccacagcg
    17101 ctgccgcaga ctgccccaca gggttatccg caggagccgg cgccgaccta cgcgccgcag
    17161 ccctaccagc agccgggtta ccccgggcag ggctaccccc agcagggtta ccagcagccg
    17221 ggttacccgc aacagggcca tccccagcaa ccgggttacc cccagcaggg ctatgccggg
    17281 gcgaccccgc ccggaccccc gacgaagcgg ccgttctaca agaagtggtg gttctggctc
    17341 atcgcggcgc tgctcgtcat cgccgtcgtc gtagctgtcg tgatcgcggc gctgcggacg
    17401 ttcggcaacg ccatcacgtc ggtcacgacg gtcacctcga ccgcccaggc aacgcccagg
    17461 ccgaccacct ccagcccgaa gccgtcgacc acgacccccc agtcgagcac gacgactacg
    17521 acgacgcccc ctcccgcgac cggcgggtcc gggcagcccg gtcgggacgg tgacctgcag
    17581 ttcacggtca ctggggtgga ctgcacgaag aacagcgtcg gcaccgcgcc gctgaccatg
    17641 aaggccgatg gggtgttctg cctcgtgtcg ttgaccatcg acaactcagg gaccaaggac
    17701 gccgacctca accccttctt ccaggaagcg accgattcga agggcgacgt tcacttcccc
    17761 gacctcgcgg cgctcgtctt catggacgat gccgacagtt tcaccgaccc ggtgcctgcg
    17821 ggcgggacgg ccaagggcat cctgccgttc gacgtgccgg ccgggtccac cctgaccagc
    17881 atcgagttgc acgagtcctt caactccccg ggcacgaaga tcgccatccc ctgagtggga
    17941 gacaatgccc gggtgagctc tgacctcgga ccgtgtcgca ccgtcaccct gctgggctcg
    18001 acggggtcga tcggcactca ggccatcacg gtcatccaag cgaacccgca gcgctatcga
    18061 gtcgtcgggc tatccgccgg agggtccgac ctccgacaga tcgccgcgca ggccgtcgag
    18121 caacaggccg aggtcgttgc ggtcgcccgg ggtgccattg acgacgtgcg cgctgcgatc
    18181 gcggcggcgg ccgcggaagc cggcgacccg gcataccagc cggaggtcct cgtcggccct
    18241 gacgcgagcc gcgagttggc cgcccgtccg gtcgacgtcg tcctcaacgg gatgacaggg
    18301 gcgatcgggc tggagcccac cctcgcggcg ctgcgcgcag ggagcacgct ggccctcgcc
    18361 aacaaggagt cgctcatcat cggcgggccg ctggtcaagg ccctcgcggc tcctgggcaa
    18421 ctggtcccgg tcgacagtga gcacagcgcg atcgctcagt gcctgcgtgg gggacgtgcg
    18481 agcgaagtac ggcggctcgt cctgaccgcg agcggcgggc cgttccgcgg tatgccgcgc
    18541 gccgacctgc acgacgtcac cccggagcag gccctcgccc accccaactt ctcgatgggg
    18601 cgggtggtga cgacgaactc ggcgaccttg gtcaacaagg ggctggaggt catcgaggcg
    18661 catctgctct tcgacgtgcc gttcgagcgg atcgccgtcg tcgtccaccc tcaacagcag
    18721 atccattcga tggtggagtt ccaggacggg tcgacgatgg ctcaggtggg gccgccgcgg
    18781 atgctcgtgc cgatcgccct cgggctgtcc tggcccgatc ggctgccgaa tgtcgacgtc
    18841 cccaccgact ggagtgtcgc ccagtcgtgg cagttcctgc cgctcgacga cgaggcgttc
    18901 cccgcggtcg cgctcgcccg ccgcgcgggc agcacgggcg gcaccgggcc ggcggtctac
    18961 aacgcggcca acgaggtgtg cgtcgacgcc ttccacgacg gggcgatccg gttcaccgac
    19021 atcgtcgaca cggtcgcgcg ggtgcttgac gggcatctgg ccggggatgc gcaggaggta
    19081 tgccgcgccg gcacggggag tgattcaccg gacctggacg ttgcaggggt gttgcgcgcc
    19141 gaccagtggg ctcgtggcga ggctcggcgg gtgctggggc tcagtgagga gcagttgtga
    19201 cgaccgtcta ctacatcgcg ggcatcctgc tgatcgtgct cggggttggg gtgtcgatcg
    19261 cgcttcacga gctcggccac ctggtgcccg cgaagcgatt cggcgtcaaa tgctcgcagt
    19321 acatgatcgg cttcggtccc accttgtggt cgcggacgat cggtgacacc caggtgggcg
    19381 tcaaggcgat cccgctcggg ggctacgtgc ggatgatcgg catgatcccg ccgcgggccg
    19441 gcgacgcgcc agggcaactg cgcagcatgt cgaccagccg gatggggacc ttggtcgacc
    19501 aggctcgcca acagtcgatg gacgagatcg agcccggcga cgagaaccgc gtcttctaca
    19561 agctgtcggt gcccaagaag gtcacggtca tgctcggcgg gccgctgatg aacctggcgc
    19621 tggccttcgt catgttgacg atcctcatgt caggcatcgg agtccagtcg ctcgtcccca
    19681 ccgtctctac cgtcgcccag tgcatcccgc agacgccgcc gacggtcgcc aagccgttcg
    19741 ccgactgcgt ggccggcgac ccgaaggcgc cctcggtggc tgctggtctg caggctggcg
    19801 acaccatcgt cgaggtgggc ggcgtgcccg ttcagttgtg ggctgaggcc accgcgaaga
    19861 tccgggcgtc ggcgggtcaa cccctggacc tcatggtcga gcgcaccggc acccgggtac
    19921 cactgaccgt ccaggtcggg caggtcgtgc ggcccacgtt caccgaagaa gggaaagtcg
    19981 ccgtccgcgc cgacggcacg gtcatcaccg agaccgtcgg ctatctgggg gcttcggcga
    20041 gcgtgggcta cgtccgccag cccctcagcg cggtgccggg ggagctcggc cggatgctcg
    20101 gcgccaccgc ctcggtgttg ctgcgaattc ccgagaagat ggtcggagtg ttcgaggccg
    20161 tcaccgggca gtccgaccgc gatcccaacg gccctgtgtc ggtcgtgggg gtcgcccgcg
    20221 ccggcggaga gatcgtcagc ggccagtacg ggccggagac gggccaatcg atcgcctacc
    20281 ggatcatcgg gctgatcttc gggctcaacc ttgcgctgtt cctcttcaac ctggtcccgc
    20341 tgctgccgtt ggatggcggt caggtcgtcg gcgcgttgtg ggagggggtc aagaagactt
    20401 gggctcggct gcgtggtcgg cccgaccccg ggtacgtcga cgtcgccaag gcactgccca
    20461 tcgcgtatgc cgtgtcgacc gttctcatcg ccatgaccct gctgctggcc tatgccgacc
    20521 tggtgacgcc gatcaaactg ggttagtcgg ctgacgcggc atacgtgcgc cgtgcggctg
    20581 tgcggcatac gtgcgctgtg cggccgtccc gcagtgcgct gtcagagcgc gatgtcggtc
    20641 gctgtgaggg cggcgaccgc ggcctgcggg ccggtgatct tgacgtcggc ggcccgcttg
    20701 cggccgtagg agtacatgac gagctcggcg actgagcccc gcacgacggc ggagcccaca
    20761 tcgctgggcc gacgtacaac ctgacggccc actccgtcgg ccacgaggat cacgcccacc
    20821 ggcgcccgcc gatagagcaa cccgcccgtg cggatcaggc ccgaccagac ggccttggcc
    20881 aggcccgggt cgaggtcccg gcggggtgtg gtggggtcac cacgaaggac atcctcgtgg
    20941 tggacgaaga actccatccg gttcatcgcc tcgtcgacgg ccggcacccc agcggccgac
    21001 caccgcggcg ggccggagcg gacccgctcg acgagctcgc cgaagtcggt cgcagcgagc
    21061 tcgtcctggg cacgttccag acgagcctgc agccgcggga cgaacatccc cgcggccagg
    21121 tcgggccggg tctcgcgaat gatgaggtgc gcggccaggt cgcggacccg ccaggggtca
    21181 cacagggtgg gggcgtccgg accgacggcg agcatcgtgt cggcaagggc gtggcgttcc
    21241 agttgcgcat agcgggtcac aaaacggatc gtgtcacacc cggggcccga cctaaggtgg
    21301 cgaaatgatc gaactgcgcc gccctgacgc gagtctgcac cgagagtggc tcgacttcgt
    21361 gtccgactac gaccgcccgg gcgtcgacgg cggcagcgtc agggacgacg agttggccgg
    21421 gctggccgat ccgggtgtgt tcgcggcctg ggtggcgcaa ctgcacgacc aagaggcggg
    21481 ccgcaacatc gcgcccgacc gagtcccctg cacgagccgg tggatcgtcc gcgagggcga
    21541 gatcgtcggg gtgatcaacc tgcgccacga actcaacgac ctccttctcg cgtggggagg
    21601 gcacatcggg tatgccgtgc gcacctcggt ccgacgacag ggcatcgcga cccgcgccct
    21661 ggccctcatg ctcggcgagg ccgcccggtt ggggatcaac cccgtcctgg tcacctgcga
    21721 cgaagacaac gtcgggtcgc ggcgcaccat cgaaggcgcg ggaggtgtct acgacgggtc
    21781 ggtcgagggc aagcgacgca actggatcac cctggcggac acccccatcg ggtatgccgt
    21841 gcgtccgctc tcgctcgacc cgctccgggg acgtctggtc gagttgcggg tcatgtcgaa
    21901 cgacgaggtg cgggccctgc actcggggga gcgtcgccca gactgggctg cggagttccc
    21961 tcggccggac gacctcgacg gttcgccgct gcgggacgtc gaggccctga aaggccgcga
    22021 gtgggtcagc cggatgatcg tgcgtcgctc ggatcaccag gtcgtgggca cgatcggctg
    22081 cttcgggcca cccgaccagg ccgacaccac gagcacggtg gaaatcggct acgggatggt
    22141 cgaatccgcc cggggtcacg ggctgatcac cgatgccttg cggttgctcg ttcccgcggc
    22201 gtcggcgcgg ggagtgacgc tccaggcgca cacggcatac gacaatcgcg cgagttgccg
    22261 cgcgctggaa cgcgcgggtt tcacgccttc cggagaggcg gacgatgctg gacgcctgcg
    22321 cttcgtacac ccgcgcgact agggtgcccg ggtgagcatc cttgaggcgc gtgacctgcg
    22381 ccggactttc acgaccacta ccggagtgct gcgccgcaac cgcaagcagg tcgaggcagt
    22441 ccgcggagtg tcgtttgcca tcgagccggg cgagctgttt gggctgctcg gtcccaacgg
    22501 tgcgggcaag acgacgacca tcaagatgct catcacgctg ctgctgccga cggcgggttc
    22561 ggcgaacatc ctcggcctcg acgtcgtccg ggacgcccgc gaggtgcgcc gccacatcgg
    22621 ctacgtcttc gggggtgacc gcggcctcta cgaacggctc tccgcccagg acaacctgcg
    22681 ctacttcgcc gagttgtatg ccgtgcccgc tcgcgaccag cgcgcccgga tcggtgagct
    22741 gctcgagctc gtcgggctca ccggccggga gaaggaacgg gtcgaagggt tctcccgagg
    22801 gatgcgccag cggctgcaca tcgcgcgtgg tctgctgcac gacccggcgt tgctcttcct
    22861 cgacgagccg tcgatcggga tcgacccggt cggcgctcgg gagttgcgcg cgaccatcgc
    22921 cgggttgcgc tcggcgggca agacgatctt gctgacgacc cactacatgt tcgaggccga
    22981 cgaactctgc gaccggatcg ccgtgctgag ccagggtgag ctcgttgcgg aagggacacc
    23041 ggcccagctc aaggctcggg tcaccgccgg gtcggtcgtc gaggtcgaga cgttcagcgg
    23101 ggtcggcccg gccgacgtcc gtgggaaggt cgatgccgtc ggtgccctgc ccggggtgcg
    23161 gtcggtcgtc gtcgatgacc ggccggacgg cgagaccgtc gtcgtgcagg tcgagccggg
    23221 ggttgatgtc acggccgccg tcctcgggac cctcaccggc atccgggtgg ggcgtgttgt
    23281 cacccgggag ccgacgttgg aggacgccta cgtccaactg gtgtcctcgt gagtggtgtc
    23341 gcgagcggct tcatgcgcgg cttcatgcgc ggcgctcggg tgctgggcgt ggggtggatc
    23401 ctgcaactga agatcctcgc tgtgtcagcc ttcgacggca tcctcaacat catctatccg
    23461 ctgttcttcg cgacgaccgc gc
//
LOCUS       DAORPW010000022        23364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_355_length_23364_cov_756.349136, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000022 DAORPW010000000
VERSION     DAORPW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 23364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23364
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_355_length_23364_cov_756.349136"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLB86_03415"
     CDS             complement(<1..598)
                     /locus_tag="PLB86_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HRC64215.1"
                     /translation="MPELPEVEVVRQGLAAHVVGRTVSDVHLTGARVARRHAAGPDDL
                     AARVRHTQVVDACRRGKYLWLVLAGPAGDRSALLLHLGMSGQLLVQPADAPDERHLHA
                     RFDFADDGPQLRFVDQRTFGGMALAELDADGIPDSISHIAPDPFEPVYDRRAVIERLK
                     ARHSAVKRGLLDQGVVSGVGNIYADEALWRAQVHGGRAS"
     gene            complement(604..1410)
                     /gene="rnc"
                     /locus_tag="PLB86_03420"
     CDS             complement(604..1410)
                     /gene="rnc"
                     /locus_tag="PLB86_03420"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRC64216.1"
                     /translation="MGQTGGGPAPSLTTLRDYLNGVIGTPVDDEVLERALTHRSYAYE
                     NGGLPNNERLEFLGDAVLGLVITDTLYAAHPDLPEGQLAKLRASVVNMRALADVGRTI
                     DVGGYVRLGRGEETTGGRDKASILADTMEAIIGTIYLSGGLRAAAEFVHHLFDPLLAT
                     AAGLGAGLDWKTSLQELCSAGSLGAPDYRLVESGPDHEKSFVAAVLVAHEVLGEGAGR
                     SKKEAEQAAAEQAWVELAARRDRAEAEARSDSDDEVDGVVEASAAGDLAD"
     gene            complement(1423..1617)
                     /gene="rpmF"
                     /locus_tag="PLB86_03425"
     CDS             complement(1423..1617)
                     /gene="rpmF"
                     /locus_tag="PLB86_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRC64217.1"
                     /translation="MAVPKRKTSRSNTRSRRANWKATAAVLTTCPRCSQPTPPHQACP
                     SCGTYKGRHVAAAERSEHQG"
     gene            complement(1619..2194)
                     /locus_tag="PLB86_03430"
     CDS             complement(1619..2194)
                     /locus_tag="PLB86_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HRC64218.1"
                     /translation="MSRLDSRAPLVFDTRELRRQPGSMQEYTRRVEVPEEMGTDIVAV
                     PAGSPVDIELRLESVSEGVLVSGSVQATATGACVRCLDPVSLPVEVSFQELFVYADRA
                     AHHHEVDADSDEAEVYELVDDLVDLQPVLRDAVVPALPFQPVCRVDCPGLCSECGVAL
                     ALDPDHHHDVLDPRWAALGTMLSDDPEENRT"
     gene            complement(2280..2768)
                     /gene="coaD"
                     /locus_tag="PLB86_03435"
     CDS             complement(2280..2768)
                     /gene="coaD"
                     /locus_tag="PLB86_03435"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRC64219.1"
                     /translation="MATICVCPGSYDPVTNGHLDVIERASTLFDEVVVAVLYNPAKTG
                     TFPVARRIELIQQALGSRPRVRVESFAGRLLVDVCQDLGAGAIVKGLRGSTDFAFELP
                     MALMNRHLTGVETVFLPGNPAYEHVSSSLIKEVHALGGDITGLVPDVVLAALREALPQ
                     RD"
     gene            complement(2790..3377)
                     /gene="rsmD"
                     /locus_tag="PLB86_03440"
     CDS             complement(2790..3377)
                     /gene="rsmD"
                     /locus_tag="PLB86_03440"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRC64220.1"
                     /translation="MTRIISGLVGGRRLATPAGVSTRPTSDRVREALFSRLEHQEVLQ
                     GARVLDLYAGSGALGLEALSRGAARATLIDSDKGAAKVARLNAQALGFGDVVTVRAEP
                     VERALLTGPDLADGRADLAFLDPPYDLAEDRLGDVLALLVLHRWLAPDALVVVERSAR
                     SPQPRWPSGLSAAGERRYGETKVWFADASGPDELA"
     gene            complement(3377..5617)
                     /locus_tag="PLB86_03445"
     CDS             complement(3377..5617)
                     /locus_tag="PLB86_03445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRC64221.1"
                     /translation="MLAAYTEDTPLRRVVKPAQAEALAKNKGLHTVGDLLEFVPRGYL
                     PPNQLTDLGELRDGEEVMVVAEVAQVSVVPMRQRRGFRCTAIVTDGRAELDLTFFSRH
                     KYDFGLRPGIWGVFAGTVTRYGTRRQLTHPEMELLDESMSAERADYLRTHQVPVYSAT
                     GKVTSFVLRQTTSLVLDCVEEVREPLPRAVRERRGLLARGDALELIHRPRLQDDVRPG
                     MRRLRYDEAFVLQTILGQRRRAAQALHTSARMPRADGLLAAFDARLPFELTAGQREVG
                     DTIAAELAADRPMHRLLQGEVGSGKTVVALRAMLAVVDAGGQAALLAPTEVLAAQHHR
                     SISALLGDLAMGGMLGGSDLGTKVALLTGSQSTPTRRSALNDAVTGDAGIVVGTHALI
                     QKHVTFFDLALVVVDEQHRFGVEQRDALREKGKTPPHLLVMTATPIPRTVAMTVFGDM
                     ETATLTELPAGRQPITTHVVDEAKPGWVERTWARVAEEVGRGHQAYVVCPKIGDPTDG
                     TTLTADTSDEDDGWDGAGDEAGEGGIPTGPQPRPLAGVYAVHDRLRSEPALAGLRTAV
                     LHGRLDADAKDRVMTAFARGEIDVLIATTVIEVGVDVPNATVMVVMDAQQFGISQLHQ
                     LRGRIGRGSAPGLCLLMTTAPTPTAAARLDAVAATTDGFELSRLDLASRREGDVLGSR
                     QSGRGSSVRFLRMGNRDDEELIAAAREDAFAVLADDPDLAEHPILRARVAARLDDEQA
                     AYLERG"
     gene            complement(5622..7331)
                     /locus_tag="PLB86_03450"
     CDS             complement(5622..7331)
                     /locus_tag="PLB86_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRC64222.1"
                     /translation="MPDQAPPPADRPRRVLSTLDAGGFLRWAAVARSSFASARAEIDA
                     LNVFPVPDGDTGTNLYLTLDSALDASRNAISRDIPVGSVTIPGLQAMCREMSRAMLLT
                     ARGNSGVILSQLFRGVAEHVVETGSEALNGAGLAGALASADRLAWRAVTEPKEGTILS
                     VSRAVSDRLRDAVDRDPTVPLAEVITLAVDTGTDALERTPSQLPALARAGVVDAGGAG
                     YLLLIEALARVIHGQPPQADDDPMRRHLGWSRPVLPARAHAESSGDDGGEGPAYEVMY
                     LLTDSDEERVAALRTRLSALGDSLLVVGAEGTWNVHVHVDDPGAAIEAGIEAGRPHRI
                     VVTHFGDQIKHALDPSALAVVACAAGEGIAEVFRAAGASVVLSGPGRRASAGQILEAI
                     RTSGAGQVLVLPNDGDTELAAQAAARAAAEEGQSVQVVRSRTAVQGIAALAVFDHGTD
                     LAANAQAMADAAAATRHGAVTIARKEALTAAGVCHVGDVLGVVQGEFAIIGSDLYDVA
                     GQVVQALVGDDGELLTVISGDDAPADLASAVVAAARIRRRDLEVTHIHGMQPLYPLLL
                     GVE"
     gene            7545..7733
                     /gene="rpmB"
                     /locus_tag="PLB86_03455"
     CDS             7545..7733
                     /gene="rpmB"
                     /locus_tag="PLB86_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRC64223.1"
                     /translation="MAATCDVCGKGPGFGHNISHSHRRTKRRWNPNIQRVKAIVNGVT
                     PKRLNVCTSCLKAGKVAR"
     assembly_gap    7792..7891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7902..8837)
                     /gene="thiL"
                     /locus_tag="PLB86_03460"
     CDS             complement(7902..8837)
                     /gene="thiL"
                     /locus_tag="PLB86_03460"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRC64224.1"
                     /translation="MTLADLTEEQLLARITPRLPGGPGVLVGPGDDTAVLRCDAATII
                     TTDAAVRGRDWRDDWSTPGDVGAKIVAQNLADVAAMGGRPLGLVVTLLADPATTVAWV
                     EGFADGLGEAARTAGAAVLGGDLSSAPPGTLAVSVTALGTLDGQAPVLRSGACAGDQV
                     AVAGTLGRSAAGLLLLERGRPELDEELVATHRRPQPPLAEGPRAGAAGAAGMLDISDG
                     LLRDGARIASASGVALDLSREALAPYAARLAPAVGADDAWECVLGGGEEHSLLACFAG
                     PVPAGWTVIGMVRTGAGVLLDGIPQAPRGWDHFRR"
     gene            8996..9229
                     /locus_tag="PLB86_03465"
     CDS             8996..9229
                     /locus_tag="PLB86_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRC64225.1"
                     /translation="MVQAYILIQTEVGKSASVAEAIRGVAGVASAEDVTGPYDVIARV
                     EANTVDELGKLVIARIQDVAGITRTLTCTVVHI"
     gene            9340..9729
                     /locus_tag="PLB86_03470"
     CDS             9340..9729
                     /locus_tag="PLB86_03470"
                     /inference="COORDINATES: protein motif:HMM:NF023453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 family protein"
                     /protein_id="HRC64226.1"
                     /translation="MSAAVVVALPAGADSAPCQALTALWPSLVSSQPARAVSVQSPAA
                     RAWGNPAVIAICGYDAPAPSTLECLSVDGIDWLVQRRSDGVQFTTYGRSPAMDVLVPA
                     AYAPEPLLLPAFGAAARSLPETGRHCQ"
     gene            complement(9752..10873)
                     /locus_tag="PLB86_03475"
     CDS             complement(9752..10873)
                     /locus_tag="PLB86_03475"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRC64227.1"
                     /translation="MSTVPPRKPRVAVVFGGRSSEHAVSCSSAASVLRAIDRDVYDVL
                     PIGITKDGRWVLAADDPAALELAPGHIPEVDGSGAGVLVPLSTTDRALQVLTPGEVPV
                     VLGDVDVVFPVLHGPWGEDGTIQGMLDLADVRYVGSGVFASAAGMDKHYMKVVFAGHG
                     LPVGPYAVITDRAWRADRGAALDAASSMGFPVFVKPARAGSSMGITRVASPAGLEAAI
                     EAAREHDPKVLVEAAIFGRELECAVLEGRGVQPPRTSHVGEIRVVTGHDFYDFEAKYL
                     SESDVELVCPADIPDDIAAEVRRLAAAAFESLGCEGLARVDCFYTEDRRVIINEINTM
                     PGFTPHSMYPRMWAETGVPYDELVDELITLALERRVGLR"
     gene            10924..>11582
                     /locus_tag="PLB86_03480"
     CDS             10924..>11582
                     /locus_tag="PLB86_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRC64228.1"
                     /translation="MTDLPPDLAAATVVVAAGRPPRSPGAPINPPIELSSTYAAHPAT
                     GPDGVALNLGYGRASNATWAAFEAAVGTLEGGDALCFASGMAAISAALTVLPADGPLV
                     APLTPYNTSGALIGEVEAAGREIRRVDVEDTVAVIAALHGARAIWLESPTNPLMQVAD
                     LPVIIAEAAARGVVVVVDNTFATPLVQQPLALGADIVVHSATKYLSGHSDLLMGVTVT
                     RA"
     assembly_gap    11583..11682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11683..12568)
                     /locus_tag="PLB86_03485"
     CDS             complement(<11683..12568)
                     /locus_tag="PLB86_03485"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRC64229.1"
                     /translation="MSTTAAVMGTGSWGTAFAAALADAGTSVTMWGRRADSVAQINAG
                     CNEDYLPGILLPEGIRASTDPQEALAGADIVVLAVPSQTLRANLSGWTLPETAAVVSL
                     MKGVELGTTKRMSEVIMEVGGVDVERVVVVSGPNLAKEIARKQPAASVVACCDQATAE
                     RVAAACATGYFRPYFNHDVVGTELGGAVKNVVALAVGMAEGMGMGDNTKASIITRGLA
                     ETARLGVRLGADPSTFMGLAGVGDLIATCMSPLSRNRTFGENLGKGMSVAEVIEVTRQ
                     TAEGVKSCHSVLDLARHAG"
     gene            complement(12565..13329)
                     /locus_tag="PLB86_03490"
     CDS             complement(12565..13329)
                     /locus_tag="PLB86_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC64230.1"
                     /translation="MRSSTSVLARMRRLPAAYQVIVVLLRPMLRVLLRRDWRGMEHLP
                     EGGCVIAPNHLSYVDPLAIALYLFDNDRPPYYLGKEAVFRIPVLGKLVGWADQIPVYR
                     GTGQAADAMRAAVAAVQEGKTVVVYPEGTLTRDPDGWPMKGKTGAARIALTTRCPVIP
                     IAMWGPQEVLGTYQKALHLFPRKTMHILAGPPVDLSDLYGKPQDVALLAMATDRIMDA
                     ITAQLEVLRGEQAPAVRFDSRAKGVPETGNPNKERP"
     gene            13416..14066
                     /gene="cofC"
                     /locus_tag="PLB86_03495"
     CDS             13416..14066
                     /gene="cofC"
                     /locus_tag="PLB86_03495"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HRC64231.1"
                     /translation="MNDVDLRADGWVVVVPAKGGDKAKSRLTAPRGTQRHTLAAAFAL
                     DTVAAALAAVGTGRVYAVTGPGPLVALLEALGAVVVPDPGGGLNAAIRAGLAHAAAEA
                     PVRVGLAVLLADLPALTADSLSVALGAAAGVERAVVPDIDGTGSVLITATGGAVPEPA
                     FGLDSAARHAAGGQQRLDLDLPRLRTDVDDTTSLRAALALGVGRHTTAALHHAATG"
     gene            complement(14125..14718)
                     /locus_tag="PLB86_03500"
     CDS             complement(14125..14718)
                     /locus_tag="PLB86_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRC64232.1"
                     /translation="MNKAELVVALEDKLGGKKAASDALDAVLDAIIREVAKGGKVAIT
                     GFGTFQKVARAARTGRNPRTGDAVRIKKTTVPRFRPGTSFKGYVADPRSLPKAAPATG
                     RAPAGSVAKAAPAAAPVKKAAPAKKAAPAKAAVKAAPAAKKAAPAKAAPVKSVATKAA
                     PAKAAPAAKKAAPAKAAPAKAAPAAKKAAPAKAAKKA"
     gene            complement(15124..15711)
                     /gene="leuD"
                     /locus_tag="PLB86_03505"
     CDS             complement(15124..15711)
                     /gene="leuD"
                     /locus_tag="PLB86_03505"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRC64233.1"
                     /translation="MDAFTTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRDGFEDGLFA
                     AWRTDESFVLNNPRYAAGSVLVAGPDFGTGSSREHAVWALMNYGFRAVLSSRFADIFR
                     GNSGKQGLLTAQVAQDDIELIWKYLEQEPGAQVTVDLAARTVTAGDIVAPFSVDDYTR
                     WRLMEGLDDIGLTLRHDDLIAQFEADRPAYLPVTS"
     gene            complement(15716..17116)
                     /gene="leuC"
                     /locus_tag="PLB86_03510"
     CDS             complement(15716..17116)
                     /gene="leuC"
                     /locus_tag="PLB86_03510"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRC64234.1"
                     /translation="MAGTLAEKVWEAHVVRRAEGEPDLLYIDLHLLHEVTSPQAFDGL
                     RLEGRTVRRTDLTLATEDHNVPTTPGPITDLISLTQVETLRRNCVEFGVPLYPMGDVE
                     QGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLPLKP
                     FRTMAVNVRGALPDGVTAKDVVLAVIAKIGTGGGQGYVLEYRGDAIESLSMEARMTVC
                     NMSIEAGARAGMIAPDDTTFDYLKGRDRAPVGADWDAAVEAWRQLRSDDDAVFDAEVD
                     LDATTLSPFVTWGTNPGQGLPLSASVPDPDRIVDETERADTERALAYMGLEPGTPLRE
                     IPVDTVFVGSCTNGRIEDLRAAADVLRGRRVAEGVRMLVVPGSARVRVQAEAEGLHSV
                     FEAAGAEWRLPGCSMCLAMNPDKLAAGERSASTSNRNFEGRQGPGGRTHLVSPLVAAA
                     TAVRGTLSSPADLDKD"
     gene            17223..17954
                     /locus_tag="PLB86_03515"
     CDS             17223..17954
                     /locus_tag="PLB86_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRC64235.1"
                     /translation="MDNSSGVGVLDKAATVLSALEAGPATLAQLVGATGLARPTAHRL
                     AVALEHHRLVTRDMQGRFVLGPRLAELASAAGEDRLLAAAGPVLGALRDHTNESAQLF
                     RRQGDLRICVAAAERPMGLRDTIPVGASLSMLAGSGAQILLAWEEPDRLHRGLQGARF
                     NATALSAVRRRGWAQSVGEREPGVASVSAPVRGPSGRVVAAVSISGPIERLSRQPGRL
                     HAATVVAGANKLTEVLRRAHQSTPS"
     gene            18084..19181
                     /locus_tag="PLB86_03520"
     CDS             18084..19181
                     /locus_tag="PLB86_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRC64236.1"
                     /translation="MTRRIGRAARVGIISLAGVLVASACTAAPTPAAAPATAVVTPVA
                     AQTSVTPTPSATPTATATPTAVPPLLAEGADGDQVRELQARLKQLKNFTSTVDGVYDA
                     ETTGAVQAFQTALKLPASGSVDETTWSTLLATTKAPSAEDLAGHLIAGPAILASGSSG
                     AKVRELQARLKQLGRWTGDVTDSYGTKTVSAVKGYQTKRGLPVTGEVDQRTMDRIWGQ
                     TRKPTSNELNNVKPAPGAGLTTAGLDPRCLVGRVICASKSTRKVVWVVDGVPVMQMDA
                     RFGRAELPTNDGAFHIYWKSIDHYSKKYDAPMPYALFFNGGEAVHYSPEFASIGYSGV
                     GSHGCVNTRDKAKMAWLFNEARVGDLVVVYR"
     gene            19275..20276
                     /gene="fgd"
                     /locus_tag="PLB86_03525"
     CDS             19275..20276
                     /gene="fgd"
                     /locus_tag="PLB86_03525"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HRC64237.1"
                     /translation="MRIGYKASAEQFGPRELVELAVLAEQVGLDSVWVSDHVQPWRHV
                     GGHAPFSLAWLAAAGERTSRVQLGTSVLTPTFRYNPAVLAQAFATLGVLNPGRIALGV
                     GTGEALNEFATGAVAEWPEFKERFARLREAIALIRTLWTEDRVTFEGDYYRTTDATIY
                     DRPEVPVPVYIAAGGPLVARYAGRAGDGFICTSGKGEELYRDQLLPAVDEGLTKAGRD
                     GVGYDRMIEVKLSWDPDRAAAVANCRFWAPLSLTAEQKHGTTDPIEMERLADELSDEQ
                     VARRWIISNDPDEVVEAVRPYVGWGFDHLVFHAPGHDQHRFLTTFAEAVLPRLRELG"
     gene            complement(20367..20439)
                     /locus_tag="PLB86_03530"
     tRNA            complement(20367..20439)
                     /locus_tag="PLB86_03530"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20403..20405),aa:Glu,seq:ctc)
     gene            complement(20541..20612)
                     /locus_tag="PLB86_03535"
     tRNA            complement(20541..20612)
                     /locus_tag="PLB86_03535"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20577..20579),aa:Gln,seq:ctg)
     gene            complement(20704..22227)
                     /gene="gltX"
                     /locus_tag="PLB86_03540"
     CDS             complement(20704..22227)
                     /gene="gltX"
                     /locus_tag="PLB86_03540"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRC64238.1"
                     /translation="MSEKQLPGAVRLRVAPSPTGDPHVGTAYMSMFDLAFARQQDGRF
                     LLRIEDTDRARLQEDSERQIYDTLHWLGLTWDEGPDIGGPCAPYRQSERLETYRPFVE
                     RLLEAGHAYLCWCSAERLTAMREDQQRRKVPTGYDRLCLGKTREERAALPGFSETPVV
                     RMQIPDDVQTSFDDLIAGRIDAPVPDDQVILKADGFPTYHLAVVVDDHLMGITHVVRG
                     QEWISSTPKHLLLYRWLGIDPPAFAHMPLLRDERKAKISKRKSPWARLTWFREQGYLP
                     EALVNFLALQGYPPIVEADGTEREVFTFDEFAARFEWSKVNPAGSIFNLDKLDWLNGV
                     WIRELPVGEFASRLLPFLEADGILSDNPSLGELARLRSIAALIQTRMVKLTEASALVA
                     PFFMGDDVIAIAEDARAQLDAQSPAVLDAAMHALGDIDDHQRGVLGSESDWSAARIEA
                     ALRSAIVEGLGVKPKVAFGPLRTAVSGQRISPPLFESMEILGKTSTLARLQALRDTV"
     gene            22321..23271
                     /locus_tag="PLB86_03545"
     CDS             22321..23271
                     /locus_tag="PLB86_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HRC64239.1"
                     /translation="MSGGLAALLDDIAAIAKLAAASIDDVGAAAGRASVKAAGVVVDD
                     TAVTPRYVQGFTPDRELPIIKRITIGSIRNKLVFILPAIMLLSQFLPWALTPLLMVGG
                     TYLCFEGAEKIWEKVSGHDAGEEAPAIVQGEDHEKTMVAGAIRTDFILSAEIMVIALN
                     EVATEPFLSRLIILIVVALGITVGVYGFVALIVKMDDIGLHLSERQSGAARAIGRGLV
                     AAMPVLLRWLAIIGTVAMLWVGGHILLVGADTLGWHGPYALVHHAEEAVAAAVSVGSG
                     ALAWLTNTLGSAIAGLIVGAVVVLIMHLIPRKKDAANAAH"
BASE COUNT         3515 a         8166 c         8048 g         3435 t
ORIGIN      
        1 ccgaggcccg cccgccatgg acctgcgccc gccagagggc ctcgtcggca tagatgttgc
       61 ccacgcccga gacgacgcct tggtcgagca gacctcgctt gacggcgctg tgccgagcct
      121 tgagccgctc gatgacggcc cgccggtcat agacaggttc gaacgggtcg ggcgcgatat
      181 gcgagatcga gtcggggatc ccgtcggcgt cgagctctgc caaagccatc ccgccgaagg
      241 tccgctgatc cacgaagcgc aactgcggcc cgtcgtcggc aaagtcgaac cgcgcatgca
      301 ggtgacgctc gtcgggggcg tcggccggct ggaccagcag ttgtccgctc atccccaggt
      361 gcagcagcag cgcgctgcgg tcaccggcgg ggcccgcaag gaccagccac aggtacttcc
      421 cgcgtcggca ggcatcgacg acctgggtat gccgcacccg cgcggcgagg tcgtcggggc
      481 cggcggcgtg gcggcgggcg acccgggcgc cggtcaggtg gacgtcggac accgtgcggc
      541 cgacgacgtg ggccgccagg ccttggcgaa ccacctcaac ctcgggcagt tcaggcatga
      601 ggcctagtcc gcgaggtcgc cggcggcgga agcctcgacg acgccgtcca cctcgtcatc
      661 gctgtcggac cgcgcttcgg cctccgcgcg gtcacggcgg gcggccaact cgacccaggc
      721 ctgctctgcg gcggcctgtt cggcttcctt cttgctgcgc cccgcccctt cgccaaggac
      781 ctcgtgggcg acgaggactg ccgcgacgaa gctcttctcg tggtccggcc ccgactcgac
      841 gagtcgatag tccggcgcac cgagggagcc tgcggagcac agctcctgca ggctggtctt
      901 ccagtccaga cccgcgccca acccggccgc agtggccagc agcgggtcga agaggtgatg
      961 cacgaactca gcagccgccc gaaggccgcc ggagagatag atcgtgccga tgatggcttc
     1021 catcgtgtcg gcaaggatcg acgccttgtc gcgtcccccg gtcgtctcct cgccgcgccc
     1081 caaacgcacg tagccaccga cgtcgatggt gcggccgaca tcggccaacg cccgcatgtt
     1141 gacgacggac gcccgcaact tggccagttg cccctcgggc agatccgggt gcgcggcata
     1201 caaggtgtcg gtgatgacca acccgagaac agcgtcgccg aggaactcca ggcgttcgtt
     1261 gttgggcagc ccaccgttct cgtacgcgta agaacggtgt gtgagggctc gttcaagcac
     1321 ctcgtcgtcg actggtgtcc cgatgacccc attgaggtag tcgcgcaggg tcgtcaggga
     1381 cggtgccggt cctcccccgg tctgtcccat gacggtgggc tatcagccct ggtgttcgga
     1441 gcgctcggcc gccgcgacgt gccgaccctt gtaggtgccg cacgacgggc acgcctggtg
     1501 cggcggggtg ggctgcgagc agcgcgggca ggtcgtcagg acggccgcag tggccttcca
     1561 gttggcccgc cgcgagcggg tgttggagcg cgaggtcttc cgcttgggga cggccacgtc
     1621 aggtcctgtt ctcttcgggg tcgtcgctca gcatcgtgcc gagcgccgcc catctcgggt
     1681 cgagcacgtc gtgatggtgg tccgggtcaa gcgcaagggc gactccgcac tcggagcaca
     1741 accctgggca gtcgacacgg cacaccggtt ggaacggcag tgcaggcacc accgcatccc
     1801 ggagcacggg ctgcagatcg accaggtcgt ccaccaactc gtacacctcg gcttcgtccg
     1861 agtcggcgtc cacctcatgg tggtgcgccg cccgatcggc atacacgaac agctcctgga
     1921 agctcacctc gactgggagg ctcacgggat ccaggcaccg cacgcatgcg ccggttgccg
     1981 tggcttggac cgaaccgctc acgaggaccc cttcggagac cgactccaac cgcagctcga
     2041 tgtcgaccgg cgaaccggcc ggcacggcga cgatgtcggt gcccatctcc tcagggacct
     2101 ctacccggcg ggtgtactcc tgcatcgacc ccggctggcg acgaagctcc ctcgtgtcga
     2161 acacgagggg cgcgcgagaa tcgaggcgag acatgacttc cgactggttg agggttcgat
     2221 ccacgccccg gacggacgta gaccgaagaa ctaggttacc ggaacggcgt cgaacgacgc
     2281 taatcgcgct gcggcaacgc ctcgcgcagg gcggccagga cgacatcggg gaccagcccg
     2341 gtgatgtcgc cgccgagcgc gtgcacctcc ttgatgaggg aggacgacac atgttcgtat
     2401 gccgggttgc ccgggaggaa gaccgtctcg accccggtga ggtggcggtt catcagcgcc
     2461 atcggaagct cgaacgcgaa gtcggtgctg ccgcgcagcc ccttgacgat cgccccggcg
     2521 ccgaggtcct ggcagacatc gacgagcagc cgacccgcga acgactcgac ccgcacccga
     2581 ggtcgtgagc cgagggcctg ctggatcaac tcgatccgac gcgcgaccgg gaacgtgccg
     2641 gtcttggcgg ggttgtagag gaccgcgacg acgacctcgt cgaagagggt cgaggcacgc
     2701 tcgatgacgt cgaggtgacc gttggtcacg gggtcataag acccggggca cacacagatc
     2761 gtcgccacgt tcgggaggct agcagccgct caggccaact cgtcgggtcc ggaggcatcg
     2821 gcgaaccaga ccttcgtctc gccgtaacga cgctcacccg cggcgctgag cccgctcggc
     2881 cagcgcggct gcggcgagcg ggcggatcgt tcgacgacga cgagcgcatc gggcgccagc
     2941 caccggtgga gcaccagcag ggcgagaacg tcgcccagtc ggtcctcagc caggtcgtag
     3001 ggcgggtcga ggaaggccag atcggcgcga ccgtcagcca ggtcagggcc ggtgagaagg
     3061 gcccgctcga ccggctcggc ccgcaccgtc accacgtcgc cgaagcccaa cgcctgggca
     3121 ttgagccgcg cgaccttggc agcacccttg tccgagtcga tgagggtcgc ccgggcggcc
     3181 cccctgctca acgcctccaa cccgagggcg ccggaaccgg catacaggtc gagcacccga
     3241 gccccctgga ggacctcctg gtgttccagg cgcgagaaga gggcctcgcg gacccggtcg
     3301 gaggtcgggc gcgtcgacac ccctgcggga gtagccaatc ggcgtccacc gacgagccca
     3361 ctgatgatgc gggtcatcac ccacgctcca gataggcggc ttgttcgtcg tccagacgcg
     3421 ctgccacccg agcgcgcagg atggggtgct ccgccaggtc ggggtcgtcc gcgagcacag
     3481 cgaacgcgtc ctcgcgggcg gccgcgatga gttcttcgtc gtcccggttg cccatccgca
     3541 ggaaccgcac cgaggagccg cgcccgctct gacgtgagcc cagcacgtcg ccctcccggc
     3601 gcgaggcgag gtccaggcgc gagagttcga agccgtccgt cgtcgccgcg acggcgtcca
     3661 aacgggccgc cgcggtgggg gtcggcgcgg tcgtcatcag cagacacagg ccaggggcgc
     3721 tccctcgccc gatccgcccc cgcaactggt gcaactgcga gatgccgaac tgctgggcat
     3781 ccatgacgac catgacggtg gcgttgggga cgtccacccc gacctcgatg acggtcgtgg
     3841 cgatgagcac gtcgatctcg ccgcgggcga acgccgtcat cacccgatcc ttggcgtcag
     3901 catcgagccg tccgtgcagg accgcggtcc gcagccccgc gagggcgggc tcggagcgca
     3961 accggtcgtg cacggcatac accccggcga gggggcgtgg ctgcggaccg gtgggtatgc
     4021 cgccctcccc cgcctcgtcg cccgccccgt cccagccgtc gtcctcgtcg gacgtgtcgg
     4081 cggtgagcgt cgtgccgtcg gtcggatcgc cgatcttggg gcagacgaca taggcctgat
     4141 gcccgcggcc gacctcctca gccacccgcg cccaggtgcg ctcgacccaa cccggcttgg
     4201 cctcgtcgac gacatgcgtc gtgatgggct ggcgaccggc cggcaactcg gtgagggtcg
     4261 cggtctccat gtcgccgaac accgtcatgg cgaccgtgcg agggatcggc gtggccgtca
     4321 tgacgagcag gtgcggaggg gttttgccct tctcgcgcaa cgcatctcgt tgctcgactc
     4381 cgaaacggtg ctgctcgtcg acgacgacga gggccaggtc gaagaacgtc acgtgcttct
     4441 ggatcagcgc gtgagtgccg acgacgatcc cggcgtctcc ggtcaccgcg tcgttgaggg
     4501 ccgagcgacg ggtcggcgtc gattggctcc cggtgagcag ggccaccttg gtgccgaggt
     4561 cggacccgcc gagcatcccg cccatcgcga gatcgccgag cagggcgctg atcgagcggt
     4621 gatgctgcgc ggccaggacc tcggtcggcg cgagcaaggc ggcctgcccg ccggcatcga
     4681 cgacggccag catggcccgc agcgcgacca cggtcttgcc cgagccgacc tcgccctgca
     4741 acagccggtg catcgggcgg tcggcggcca actcggcggc gatcgtgtcg ccgacctcgc
     4801 gctggcccgc ggtcaactcg aacggcagcc gggcgtcgaa ggccgcgagg agaccgtcag
     4861 cacgcggcat acgggcgctg gtgtgcagcg cctgcgcggc gcggcggcgc tggccgagga
     4921 tcgtctgcag gacgaaggcc tcgtcgtagc gcaagcggcg catccccggc cggacgtcgt
     4981 cctgcaggcg gggccggtgg atgagctcga gcgcgtcgcc tcgggccagc agtccgcggc
     5041 gctcccggac cgcgcgcggg agcggctcgc ggacctcctc cacacagtcc aggaccagcg
     5101 aggtcgtctg acgcagcacg aacgaggtga ccttgccggt cgccgaatag acgggcacct
     5161 gatgggtgcg caggtagtcg gcgcgttcgg ccgacatgct ctcgtcgagc aactccatct
     5221 cgggatgggt gagttgacgg cgggttccgt aacgggtgac cgtgccggcg aagacacccc
     5281 agatgcccgg ccgcagccca aagtcgtatt tgtgccggct gaagaaggtc aggtcgagct
     5341 cagcgcgacc gtcggtgacg atcgcggtgc accggaagcc ccgacgttgg cgcatcggga
     5401 ccaccgacac ctgggcgacc tcggcgacga ccatgacctc ctcgccgtcg cgcaactcgc
     5461 cgaggtcggt cagttggttg ggcgggaggt aaccgcgcgg gacgaactcc aacaggtcgc
     5521 cgaccgtgtg caggcccttg ttcttggcca gcgcctcggc ctgagcgggt ttgacgaccc
     5581 gtcgcagcgg ggtgtcctcg gtgtatgccg cgagcatcgg ctcactcgac gccgagcagc
     5641 agcgggtaga gcggctgcat gccgtggatg tgggtcactt cgaggtcgcg gcggcggatg
     5701 cgagcagcgg cgaccacggc ggaggccagg tcggcgggag cgtcgtcgcc ggagatcacg
     5761 gtgagcagct cgccgtcgtc acccacgagc gcctggacga cctggccggc aacgtcgtag
     5821 aggtcagacc cgatgatcgc gaactcccct tggaccacgc cgagcacgtc gccgacatga
     5881 cagaccccgg ccgcagtgag ggcctccttg cgggcgatgg tcacagcgcc gtggcgggtc
     5941 gcggccgcag catcggccat cgcctgcgcg ttggcggcca gatcggtgcc gtggtcgaag
     6001 acggcaagcg cggcgatccc ctggacggcg gtgcgcgacc gaaccacctg gaccgactgg
     6061 ccttcctccg ccgcggcgcg tgcggcggcc tgcgccgcca gctcggtgtc accgtcgttg
     6121 ggcagcacga ggacctggcc cgcgccgctg gtgcggatcg cctcaaggat ctggccggcc
     6181 gaggcccgtc gcccggggcc actgaggacg accgaggcgc ccgcggcgcg gaacacttcg
     6241 gcgatcccct cgcctgcggc acaggcgacg actgcgagcg ccgaggggtc gagcgcgtgc
     6301 ttgatctggt cgccgaagtg cgtgacgacg atccggtgcg gacggccggc ctcgatgccc
     6361 gcctcgatgg cggctccggg gtcatcgaca tggacgtgga cgttccacgt gccctcggcg
     6421 ccgacgacca gcagggaatc acccagcgct gacaggcggg tccgcagggc agcaacccgc
     6481 tcctcgtcgc tgtcggtcaa cagatacatg acctcgtatg ccggtccctc accgccgtcg
     6541 tcaccgctcg actcagcgtg ggcccgggcc gggagcaccg gccgtgacca ccccaggtgt
     6601 cgtcgcatcg ggtcgtcgtc cgcctgcggc ggctggccgt ggatcacccg ggccagggcc
     6661 tcgatgagca ggagatagcc ggcgccacca gcgtcgacga ccccggcgcg agccagggca
     6721 ggcagctggg acggcgtgcg ttccaaggcg tcggtaccgg tgtccacggc aagtgtgatg
     6781 acctcggcca gaggcacggt ggggtcgcgg tcgaccgcat cgcggagccg gtcggacacg
     6841 gcccgggaca ccgacaggat ggtgccttcc ttgggctcgg tgacggcccg ccaggccagc
     6901 cggtcggcgc tggctagtgc cccggctagg cccgcgccgt tcagggcctc gctgcccgtc
     6961 tcgacgacgt gctcggcgac gccccggaac agttggctga ggatgacacc cgagttgccg
     7021 cgggccgtga gcaacatggc gcgcgacatc tcacggcaca tcgcttgcag tccggggatc
     7081 gtcaccgatc cgacggggat gtctcgcgaa atcgcattgc gtgaggcgtc cagggctgag
     7141 tcgagggtga ggtagaggtt ggtgcccgtg tcaccgtcgg gcaccgggaa gacgttgagg
     7201 gcgtcgatct cggcacgcgc ggacgcgaac gacgaccggg cgacggctgc ccatcgaagg
     7261 aatcccccgg cgtcgagggt gctcagcacc cgccgggggc ggtccgccgg aggcggggct
     7321 tggtcgggca tcggacctcc ttgacgcgaa ccggggccgc gagacacggc cagcctagtc
     7381 agcggtcggt cgtcgttttg gccccggcgc gcccgctcgg ctattcttgg caagttgccc
     7441 tgttcggtct gggcgtgccc gctgggcacg cggcataccg gcgcggggcc cccgaccttc
     7501 cagaccgcgg gttcgacccc gcatcagagc atcaggagag acccgtggca gccacttgcg
     7561 acgtttgcgg caagggaccg ggcttcggtc acaacatctc gcactcgcac cggcgcacca
     7621 agcgtcgctg gaacccgaac atccagcggg tcaaggccat cgtcaacggt gtgaccccca
     7681 agcgcctcaa cgtgtgcacc tcgtgcctca aggccgggaa ggtcgcgcgc tgagcgcctg
     7741 acccatcggt tgcagaaggc cggctccccc atcgggagcc ggccttctgc tnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggaaggggc gtcagcggcg gaagtggtcc
     7921 cacccacgag gagcctgggg tatgccgtcc agcagcaccc cagccccggt ccggaccatg
     7981 ccgatcaccg tccaccccgc aggcaccggg cccgcgaagc acgccaacag cgagtgctcc
     8041 tcaccgcccc ccagcacgca ctcccaggcg tcgtcggcac cgacagcggg agccaaccgc
     8101 gcggcatacg gtgcgagggc ctcgcgcgac aggtcgagag cgaccccgct ggcggaggcg
     8161 atccgggcgc cgtcgcgaag cagcccgtcg gagatgtcga gcatccccgc agccccggct
     8221 gcgccagccc gaggcccttc ggccagcggc ggttgcggtc ggcggtgggt cgcgaccaac
     8281 tcctcgtcga gctcgggtcg gccccgctcg agcagcagga gaccggcagc cgagcggccc
     8341 agcgtgcccg ccaccgcgac ctggtcgccc gcgcaggccc cggagcgcag cacgggcgcc
     8401 tgaccgtcga gcgttccgag cgccgtgacc gagacggcca gcgtccccgg cggagccgag
     8461 gacaggtcgc cgcccaggac ggcggctccg gccgtgcgcg cggcctcccc cagtccatcg
     8521 gcgaacccct cgacccaggc gaccgtcgtc gccgggtcgg ccagcagggt gacgaccagc
     8581 ccgagcggcc gtcctcccat cgcggccacg tcggcgaggt tctgggcgac gatcttcgct
     8641 ccgacgtcac ctggcgtcga ccagtcgtcg cgccagtccc ggccgcggac ggcagcgtcg
     8701 gtcgtgatga tcgtcgcggc gtcgcaccgg agcacggccg tatcgtcacc cggcccgacg
     8761 aggacacccg gaccgccggg cagccgggga gtgatgcggg cgaggagttg ctcctcggtc
     8821 agatcggcga gcgtcatccc cgccgccttg ccccgtgcac gcctacctgg cacgaccttc
     8881 tcctcctgct caagacgacg aaaccccgcc cagcacggta gcgtctgggg gacgggtcga
     8941 ggacgacccg cgcgcgggca ctgcccgcca gacccccgac aagtgaggtt gctctgtggt
     9001 ccaggcatac atcctgatcc agaccgaggt cggcaagtcg gcgtcggtcg ccgaggcgat
     9061 ccgcggggtt gcgggcgtcg catccgccga ggacgtcacc ggcccctacg acgtcatcgc
     9121 ccgggtcgag gccaacacgg tcgatgagct cggcaaactc gtcatcgccc gcatccagga
     9181 cgtcgcgggc atcactcgga cgctcacctg cactgtggtg cacatctgag cggtcgctcg
     9241 ctgcggtcac cagggcggca gaccggcatc ggccggtatg ccgccctgct cgctgtcccg
     9301 ctcggcctcc tcggcgccgt tggcgcctgt tccaacccgg tgagcgcagc ggtggtcgtg
     9361 gccctgccgg caggagcgga cagcgccccc tgccaggcct tgacggccct ctggccgagc
     9421 ctggtgtcgt cgcagcccgc gcgagccgtg agcgtgcagt cgccggcggc ccgggcgtgg
     9481 ggcaacccag ccgtcatcgc catctgtggt tacgacgccc ccgcaccgtc gaccttggag
     9541 tgcctgtccg tcgacggcat cgactggctg gtccaacgac ggtccgatgg agtgcagttc
     9601 accacctacg gccgcagccc ggcgatggac gtcctggtgc ccgcggcata cgcccctgag
     9661 ccgttgttgc tgcccgcctt cggggcggcg gcccggtccc tgcccgagac cggtcgccac
     9721 tgtcagtagc tcccgaggtg cccgcgcagc cttaacgaag gccgacgcgg cgctccaacg
     9781 ccagggtgat gagttcgtcg acgagttcgt cgtaggggac gccggtctcg gcccacatcc
     9841 gcgggtacat cgagtgtggg gtgaagccag gcatcgtgtt gatctcgttg atgatcaccc
     9901 gacgatcctc ggtgtagaag cagtccaccc gggccagccc ctcgcagccc agggattcga
     9961 aggccgccgc cgccaggcgc cgaacctcgg cggcgatgtc gtcggggatg tcggccgggc
    10021 agaccagttc cacatcgctc tcggagaggt atttcgcctc gaagtcatag aagtcgtggc
    10081 cggtgacgac ccggatctcg ccgacgtgcg acgtccgcgg cggctgcacg cctcgaccct
    10141 cgaggacggc gcattccaac tcgcggccaa agatcgccgc ctcgacgagc accttcgggt
    10201 cgtgctcgcg ggccgcctcg atggccgcct cgagtccagc gggtgaagcc acccgagtga
    10261 tccccatgct cgacccggcc cgagcaggct tgacgaagac cgggaagccc atcgacgagg
    10321 cggcgtcgag ggccgcgccg cggtcggctc gccaagcgcg gtcggtgatg acggcatacg
    10381 ggccgacggg gaggccatga ccggcgaaga cgaccttcat gtagtgcttg tccatgcctg
    10441 cggcagaggc gaatacgccg gaaccgacgt agcgcacgtc ggccagatcg agcatcccct
    10501 gaatcgtgcc gtcctccccc cacgggccgt ggagcacggg gaagacgacg tcgacgtcac
    10561 cgaggacgac cggcacctcg cccggtgtga gcacctgcag ggcccgatcg gtcgtcgata
    10621 gcggcacgag cacccccgcg cccgacccgt cgacctccgg gatgtgcccg ggagccagtt
    10681 cgagggcggc cgggtcgtcg gcggccaaca cccagcgtcc gtccttggtg atgccgatgg
    10741 gcaggacgtc atagacgtcg cggtcgatgg cccgcagcac cgaggccgcg ctgctgcacg
    10801 acacggcgtg ttcggaggac cgtccgccga agacgacggc gacgcgcggt ttacggggcg
    10861 ggacagtgct catcggcaac gaccctactg ccccggtatg ccgtgtccgc ctaggctggc
    10921 cggatgaccg acctgccccc ggacctggcc gccgccaccg tcgtcgtcgc cgcggggcgc
    10981 cccccacgca gccccggtgc accgatcaat ccgccgatcg agttgtccag cacgtatgcc
    11041 gcccaccccg ccaccggccc cgacggggtg gccctgaacc tcggctacgg ccgggcatcg
    11101 aacgccacct gggcggcttt cgaagcggcc gtcgggaccc tcgaaggtgg cgacgcgctg
    11161 tgtttcgcct ccggcatggc ggcgatctcg gccgccctca ccgtcctccc ggcggacggc
    11221 ccgctggttg ctcccctgac gccctacaac acctccggtg cgctcatcgg ggaggtggag
    11281 gccgcgggcc gcgagatccg ccgggtcgac gtcgaggaca ccgtagcggt catcgcggcg
    11341 ctgcacggtg cccgcgcgat ctggctggaa tcgccgacca accccctcat gcaagtagcc
    11401 gacctgccgg tcatcatcgc cgaagccgct gcgcgcgggg tcgtcgtcgt cgtcgacaac
    11461 accttcgcca ctcccctggt gcagcaaccg ctggccctcg gcgccgacat cgtcgtccac
    11521 tcggcgacga agtacctctc cgggcactcc gacctgctca tgggggtcac ggtcacccgc
    11581 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgccggcg tgccgcgcca
    11701 ggtcgagcac cgagtgacac gacttgacgc cttcggcagt ctggcgagtc acctcgatga
    11761 cttcggcgac cgacatcccc ttgccgaggt tctcgccgaa ggtgcggttg cgggagaggg
    11821 gagacatgca ggtggcgatg aggtcgccca caccagccaa acccatgaag gtcgacgggt
    11881 ccgcccccaa gcgcacaccg agccgcgccg tctcggccaa gccgcgggtg atgatcgacg
    11941 ccttggtgtt gtcgcccatc cccatgcctt cggccatgcc gacagcgaga gcaacgacgt
    12001 tcttcaccgc cccgccgagc tcggtcccga cgacgtcatg gttgaagtaa ggccggaagt
    12061 agccggtcgc gcaggcagcg gccacgcgct ccgcggtcgc ctggtcacaa caggccacga
    12121 cgctcgcggc aggttgtttg cgggcaatct ccttggccag gttgggcccg gaaacgacga
    12181 cgacacgctc gacgtcgacc cccccgacct ccatgatgac ctcgctcatc cgcttggtcg
    12241 tgccgagttc gacacccttc atcagcgaga cgacggcggc cgtctcgggc agcgtccagc
    12301 cgctgaggtt ggcgcgcagc gtctgggaag ggaccgcgag gacgacgatg tcggccccgg
    12361 ccaacgcctc ctgcgggtcg gtgctggccc ggatgccctc cggcagaagg attccgggga
    12421 ggtagtcctc gttgcagccg gcgttgatct gcgccaccga gtcggcccgc cggccccaca
    12481 tggtgacgct cgtgccggcg tcggccaatg ccgcggcgaa cgcggtcccc cagctacctg
    12541 ttcccatgac cgcggcggtg gtgctcatgg acgctccttg ttggggttgc cggtctcggg
    12601 gacacccttg gcccgcgagt cgaaccgcac agcgggggcc tgctcgcccc gaaggacttc
    12661 cagttgggcc gtgatggcgt ccatgatccg gtcggtggcc atggcgagca aggcgacatc
    12721 ctgcggcttg ccgtagaggt cgctcaggtc cactggcggg ccggccagga tgtgcatggt
    12781 cttacgaggg aagaggtgca gggccttctg gtaggtcccg aggacttcct gcggacccca
    12841 catcgcgatg gggatgaccg ggcagcgcgt ggtcagggcg atccgagcgg cgccggtctt
    12901 gcccttcatc ggccagccat ccgggtcgcg ggtcagcgtc ccctccgggt agacgacaac
    12961 ggtcttgccc tcctgcaccg ccgccaccgc ggcccgcatg gcgtcggctg cctgccccgt
    13021 cccgcgatag accgggatct ggtcggccca accgaccaac ttgcccagga ccgggatccg
    13081 gaagacggct tccttgccga ggtagtaggg cggccggtcg ttgtcgaaga gatagagcgc
    13141 aatggccaac gggtcgacat aggacagatg gttcggggcg atgacgcatc cgccctcggg
    13201 aaggtgctcc atgccccgcc agtcccgacg gagcagcaca cgcagcatcg gtcgcagcag
    13261 cacgacgatg acctggtatg ccgccggcag ccgccgcatt cgcgccagca cggacgtgga
    13321 cgacctcaca cccaactcct tctcgacgcc cgtatccttg cggcccgggg tcccagtgtc
    13381 gagcatgcac caccggaggg gagacgatgt cgctcgtgaa tgacgtggac ctccgcgccg
    13441 acggctgggt ggtcgtcgtg cccgccaagg gcggcgacaa ggcgaagtcc cgcctcaccg
    13501 caccgcgggg gacgcagcgg cataccctcg cggcggcctt cgcgctggac accgtcgccg
    13561 cggcactcgc tgcggtcggc acggggcggg tgtatgccgt gaccggcccc ggcccgctcg
    13621 tcgccctcct ggaggcgctg ggtgccgtcg tggtccctga ccccggcggc gggctcaatg
    13681 cggccatccg cgcgggcctg gcgcacgctg cggccgaagc accggtgcgc gtcgggttgg
    13741 ccgtgctgtt ggccgatctt cccgcgctga ccgccgactc cctgagcgtg gcgttgggcg
    13801 ctgccgccgg tgtcgagcgg gccgtcgtgc cggacatcga cggaaccggt tcggtgctca
    13861 tcacggcgac aggcggggct gtccccgagc cggcgttcgg cctcgactcg gcggcccgac
    13921 acgccgcggg cgggcagcaa cgcctcgacc tcgacctgcc gcggctgcgg accgacgtgg
    13981 acgacacgac ctccctgcgg gcggcgctgg cgttgggggt gggacggcat acgacagccg
    14041 ccctgcacca cgcggccacg ggctgacacg agcacgggcc ggtctctccc gcaggagaga
    14101 ccggcccggt cgaggctcgg cggttcaggc cttcttcgcc gccttggcgg gagcagcctt
    14161 cttggcagcc ggagcggcct tggcgggagc cgccttggcc ggggcggcct tcttggcagc
    14221 cggcgcagcc ttggccggag cggccttcgt cgcgaccgac ttcacgggag cagccttggc
    14281 aggtgcggcc ttcttggcgg cgggcgcagc cttgacggcg gccttggccg gcgcggcctt
    14341 cttggccggc gcggccttct tcaccggcgc agcggccgga gcagccttgg caaccgagcc
    14401 ggccggggcc cggccggtgg ccggagcagc cttgggcagc gagcgcgggt cggcgacgta
    14461 gcccttgaac gaggtgcccg ggcggaaccg cgggacggtc gtcttcttga tccggacggc
    14521 gtcacccgtg cgggggttgc gcccagtgcg agcggcccgc gccaccttct ggaacgtacc
    14581 gaacccggtg atggcaacct tgccgccctt ggcgacctca cgaatgattg cgtccagcac
    14641 cgcgtcgagt gcatcgctgg cggccttctt gccgccgagc ttgtcttcga gtgctacgac
    14701 gagctctgcc ttgttcacgc ttcccctcca gatgaacgcc ccggatccag ccgggtgtcg
    14761 atatcgcgga cgggtcgtcc gccttggacg acggtattcc cgtccaggcc tggagtccac
    14821 catctcgggt tgcgattccc gttgccgcgc aaggtatttg ctctcacgaa gcggagtttt
    14881 cgacgcgtgc tcacaagttg tgaactcgct ttggccaccc cacgacgccg cgcttccccc
    14941 aaagaggttg tccagcaacg cttttgggca cccgatcagc agatctcagc ggaagcgaag
    15001 gcgtcgccac cctgcacggg gccattgcgt ggaccacgca ggcgtgtggc gtatctccca
    15061 gtgcgacacg ccgactcggc gtgtcgcacg gttcgcccgg ttcgtccgag agaccgcagc
    15121 gcttcagctc gtgacgggga ggtatgccgg tcggtcggct tcgaactgcg cgatgaggtc
    15181 gtcgtggcgc agggtgaggc cgatgtcgtc cagcccctcc atgagccgcc agcgggtgta
    15241 gtcatcgacg ctgaatggcg cgacgatgtc accggccgtc accgtccggg ccgcgaggtc
    15301 gacggtcacc tgggcacccg gctcctgctc gaggtacttc cagatgagct cgatgtcgtc
    15361 ctgggccacc tgcgccgtga gcagtccctg cttgcccgag ttgccgcgga agatgtcggc
    15421 gaaccgcgag gacagcacgg cccggaagcc gtagttcatc agggcccaca cggcgtgttc
    15481 gcgggaggac ccggtgccga agtcgggacc cgcgacgagg accgaccccg cggcataccg
    15541 cgggttgttg aggacgaacg actcgtcggt gcgccaggcg gcgaagaggc cgtcctcgaa
    15601 cccgtcgcgc gtcactcgct tgaggtagac cgcagggatg atctggtcgg tgtcgacgtt
    15661 gctgcgccgc agcgggacgc cgatgccggt gtgcgtcgtg aaggcgtcca tgcgctcagt
    15721 ccttgtccag gtcggccggc gaggagaggg tgccgcgaac ggcggtggct gcggccacga
    15781 gggggctcac caggtgggtg cgaccaccgg ggccctggcg accttcgaag ttgcggttcg
    15841 aggtcgacgc gctgcgctcg ccggcggcga gtttgtcggg gttcatggcc aaacacatcg
    15901 agcagccggg cagacgccac tcggcgccag cggcctcgaa gaccgagtgc agaccctcgg
    15961 cctcggcctg cacccgcacc cgcgccgatc ccgggacgac aagcattcgg accccctcgg
    16021 cgacccggcg gccgcgaagc acgtcggctg ccgcccgcag gtcctcgatc cggccgttgg
    16081 tgcacgaccc gacgaagacg gtgtcgacgg ggatctcgcg cagcggcgtc cccggctcga
    16141 ggcccatgta ggccagggcg cgctcggtgt cggcgcgctc ggtttcatcg acgatccggt
    16201 ccgggtcggg aacgctcgcc gacaggggca ggccctgccc ggggttggtg ccccaggtga
    16261 cgaacgggct cagggtggtg gcgtccaggt cgacctcggc gtcgaacacg gcgtcgtcgt
    16321 cgctgcgcaa ctggcgccac gcctcgacgg cggcgtccca atcggcaccc accggcgcgc
    16381 ggtcgcggcc cttgaggtag tcgaaggtgg tgtcatcggg cgcgatcatc ccggcgcgag
    16441 ccccagcctc gatcgacatg ttgcagaccg tcatccgggc ttccatcgac aacgactcga
    16501 tggcgtcgcc gcggtattcc aggacgtatc cttgcccacc gccggtgccg atcttggcga
    16561 tgaccgccag gacgacgtcc ttcgcggtca ctccgtcggg gagggcgccg cggacattga
    16621 ctgccatcgt gcggaacggc ttgagcggca gggtctgggt ggcgagcaca tgctcaacct
    16681 ccgaggtgcc gatcccgaag gcaagggcgc cgaaggcacc atgggtcgag gtgtggctgt
    16741 caccgcagac gatggtcatc cccggctggg taagccccaa ctgcgggccg acgacgtgga
    16801 cgatgccctg ctcgacgtcg cccatcggat agagcggcac cccgaactcg acacagttgc
    16861 gccgcagggt ctcgacctgg gtgaggctga tgagatcggt gatcgggccc ggcgtcgttg
    16921 ggacgttgtg gtcctcggtc gccagggtga ggtcggtccg gcgcacggtg cggccctcaa
    16981 gccgaaggcc gtcgaacgcc tgcgggctgg tcacctcgtg caggaggtgc aggtcgatgt
    17041 agagcaggtc cggctcgcct tcagcgcggc gcacgacatg cgcctcccag accttctcag
    17101 ccagcgtccc ggccatcccg ccaccttccc aagccatctt catacgcctg tcgatgccag
    17161 catccgacac cgttccgcac ggtggatttg cgtctcacgc cctgagacgg caatatcagt
    17221 gcatggacaa ctcaagcggg gtcggcgttc tggacaaggc tgccactgtc ttgtcggcgt
    17281 tggaagcggg tccggccact ctcgcccaac tcgtcggggc gactgggctg gctcgaccga
    17341 ccgcccaccg gctcgcggtg gcgctcgagc accaccgcct cgtgacgcgc gacatgcagg
    17401 ggcgtttcgt cctcggcccg aggctggccg agctggcgtc ggccgccggc gaggaccgcc
    17461 tgctcgccgc tgcgggcccg gtgctgggtg ccttgcgcga ccacaccaac gagagtgccc
    17521 aactcttccg ccgtcagggc gacctgcgca tctgcgtcgc ggccgccgag cgtccgatgg
    17581 ggctgcgcga caccatcccg gtcggggcga gcctgtccat gctcgccggc tccggcgccc
    17641 agatcctcct cgcgtgggag gagcccgacc gcttgcatcg cgggctgcag ggtgcgcgtt
    17701 tcaacgccac cgcactgtcg gccgtccgcc gtcgcggctg ggcgcagagc gtcggtgagc
    17761 gcgagcctgg ggtggcatcg gtctcggcgc cggtccgcgg tccgtccggt cgggtggtgg
    17821 ccgcggtgtc gatctcgggg ccgatcgagc ggctctcccg gcagccgggg cgcttgcacg
    17881 cggcgaccgt ggtcgccggc gcgaacaaac tcaccgaagt gttgcgccgg gcgcaccaga
    17941 gcacaccgag ctagtctgag ttgaggtatg ccgccctggc agaccgctcg tcgctccggt
    18001 tgttcaccct gaccggatca acctgtcgga gacctggggc gtcatgagag cagcagttcg
    18061 gcacgcttgg caggagacga agagtgacac gacgtatcgg cagagccgcg cgagtgggga
    18121 tcatctcgct ggcgggcgtg ctcgtcgcct ccgcctgcac cgcagccccg acgcccgccg
    18181 cagcaccggc gaccgccgtc gtcacccccg tggcggcgca gacgtccgtc acgccgaccc
    18241 cttcggcgac cccgacagcc accgccacgc cgaccgccgt ccctccgctg ctggccgaag
    18301 gtgccgacgg cgaccaggtg cgcgagctcc aggcccggct caagcagctc aagaacttca
    18361 cgtccaccgt cgacggtgtc tatgacgccg agaccacggg cgcggtgcag gctttccaga
    18421 cggccctcaa actcccggcg tccggatcgg tcgatgagac cacctggtcg actctcctag
    18481 cgacgaccaa ggctccttcg gccgaggacc tggccgggca tctcatcgcc gggccggcca
    18541 tcctcgcgtc cgggtcctcc ggagccaagg tccgggagtt gcaggcccgg ctcaagcagc
    18601 tcggccggtg gaccggcgat gtcaccgact cctacggcac caagacggtt agcgcggtca
    18661 agggctacca gaccaagcgc ggccttccgg tgacgggcga ggttgaccaa cgcacgatgg
    18721 accgcatctg ggggcagacc cgcaagccca cgagcaacga gctcaacaac gtcaagcccg
    18781 cgccgggtgc cgggctgacc accgccgggc tcgacccgcg ctgcctggtc ggccgagtga
    18841 tctgcgcgtc caagtcgacg cgcaaggtcg tctgggtcgt cgatggcgtt cccgtcatgc
    18901 agatggacgc gcggttcggg cgggcggagt tgccgacgaa tgacggcgcc ttccacatct
    18961 actggaagtc gatcgaccac tactccaaga agtacgacgc gcccatgccc tacgcgctct
    19021 tcttcaacgg tggcgaggcc gtccactact cccccgagtt cgcctccatc ggctactccg
    19081 gcgtgggctc gcacgggtgc gtcaacaccc gcgacaaggc gaagatggcc tggctgttca
    19141 acgaagcgcg cgtcggggat ctcgtcgtcg tctaccgctg acaccggcgc ggccgccggt
    19201 aaggtgcgct cacggcatac cgcaggggta tgccgtgcgg cggcctccac cgccacgtca
    19261 ccccgggagt tggcatgcgg atcggctaca aggcgtcggc agagcagttc ggtccgcgtg
    19321 agctcgtcga gctcgccgtg ctggccgagc aggtcgggct ggactcggtc tgggtctccg
    19381 accacgttca gccctggcgt cacgtgggcg ggcacgcacc cttctcgctg gcctggctag
    19441 ccgcggcggg cgagcgcacc tctcgagtcc agttggggac ctcggtgctc accccgacct
    19501 tccgctacaa ccccgctgtg ctggctcagg cgttcgccac ccttggtgtc ctcaaccccg
    19561 gccggatcgc cctcggcgtc ggcaccggcg aggcgctcaa cgagttcgcg acgggcgcgg
    19621 tggccgagtg gccggagttc aaggagcgtt tcgcccggct gcgcgaggcg attgcgctca
    19681 tccgcaccct ctggaccgag gaccgggtga ccttcgaagg cgactactac cgcaccaccg
    19741 acgcgacgat ctacgaccgg cccgaggtcc ccgtccccgt ctacatcgcc gcgggcgggc
    19801 cactggtcgc ccggtatgcc ggtcgcgcgg gtgacgggtt catctgcacc tccgggaagg
    19861 gcgaggagct ctaccgcgac caactgctac cggctgtcga cgagggcctg acgaaggccg
    19921 gtcgcgatgg ggtcggctac gaccggatga tcgaggtcaa gctctcctgg gaccccgatc
    19981 gcgcggccgc ggtggccaac tgccggttct gggcgccgct gtcgctgacc gccgagcaga
    20041 agcacggcac gaccgacccg atcgagatgg agcggctggc cgacgagctc agcgacgagc
    20101 aggtggctcg gcgctggatc atcagcaacg accccgacga ggtcgtggag gcggtccgcc
    20161 cctacgtcgg ctggggcttc gatcacctgg tcttccacgc tcccgggcac gaccagcacc
    20221 ggttcctgac gaccttcgcg gaggcggtcc tgccgcggct gcgcgaactg ggctgaccgg
    20281 catacccact gggcggcccg acccacctgc ctggacatgc agaacggccc ctgaccgatg
    20341 tgtcggtcag gggccgttct cgcgcgtagc cccgacggga ttcgaacccg cgctaccgcc
    20401 ttgagagggc ggcgtgctag gccgctacac aacggggccc tagcgatgtc gtccgagggg
    20461 tctcctcgtc ggacacaggt ggaaacctta ccggtgaccg ggggcttcca ccgaatcggc
    20521 cgcccactgg gcggccgaag ctggggtacc aggactcgaa cctagaattg cggaaccaga
    20581 atccgacgtg ttgccaatta caccataccc catggggtat acctcccgtt cgcccggaag
    20641 cgatcgggaa gtgaaccgaa gagcgatact accggcccag cagagcctcg cccaaatccc
    20701 cggtcagacg gtgtctcgca acgcctgcaa ccgggccagg gtggaggtct tcccgaggat
    20761 ctccatcgac tcgaacaggg gcggcgagat gcgctgaccc gaaaccgcgg tgcgcagcgg
    20821 cccgaaggcc accttgggct tgaccccgag accttcgacg atcgccgaac gcagggcagc
    20881 ctcgatccga gcggcgctcc aatcggactc cgacccgagc acgccccgct gatggtcgtc
    20941 gatgtcgccg agggcatgca tcgcggcgtc caggaccgcc ggggactggg catcgagttg
    21001 ggcgcgcgcg tcttcggcga tggcgatgac gtcatcgccc atgaagaagg gcgccacgag
    21061 ggcgctagcc tcggtcaact tgaccatccg ggtctggatg agcgccgcga tggaccgcaa
    21121 ccgggccaac tcgcccagcg acgggttgtc cgacaggatc ccgtcggcct cgaggaacgg
    21181 cagcaaccgg gaggcgaact cccccaccgg caactcgcgg atccacacgc cgttgagcca
    21241 gtcgagtttg tcgaggttga agatcgaccc cgcagggttg accttcgacc actcgaagcg
    21301 cgccgcgaac tcgtcgaagg tgaagacctc gcgctcggtt ccatccgcct cgacgatcgg
    21361 cgggtagccc tgcagggcaa ggaagttgac aagggcctcg gggaggtagc cctgctcccg
    21421 gaaccacgtc agccgggccc aggggctctt gcgcttggag atcttcgcct tgcgctcgtc
    21481 gcgcagcaac ggcatgtgag cgaaagccgg cggatcgatg cccagccacc gatagagcaa
    21541 caggtgcttg ggcgtcgagg agatccactc ctggccgcgc acgacgtggg tgatccccat
    21601 gaggtgatcg tcgacgacga cggccaggtg gtaggtcggg aagccgtcgg ccttgaggat
    21661 cacctggtcg tcagggaccg gcgcgtcgat gcgcccggcg atgaggtcgt cgaaagaggt
    21721 ctggacgtcg tcagggatct gcatccgcac gaccggcgtc tcggagaagc cgggcagggc
    21781 ggcgcgttcc tcgcgggtct tgcccaggca cagtcggtcg tagccggtcg gcaccttgcg
    21841 tcgttgttgg tcctcacgca ttgccgtgag ccgctcggcg gagcaccaac atagataggc
    21901 gtggcccgcc tcgagcagtc gctccacgaa cggccggtag gtctccaacc gctcggactg
    21961 tcggtagggc gcgcacgggc cgccaatgtc gggcccctcg tcccacgtca gccccagcca
    22021 gtgcagggtg tcgtagatct gccgctcgct gtcctcctgc aaccgcgcac ggtcggtgtc
    22081 ttcgatccgc aggaggaaac gcccgtcctg ttggcgcgcg aacgcgagat cgaacatcga
    22141 catgtatgcc gtgccgacgt ggggatcgcc ggtgggcgac ggggcgacgc gcagacgaac
    22201 cgcgcccggt agctgcttct cactcatggt ggacgtcagc ctagccgcgc ggcgagccaa
    22261 ctagtctgtc cgtgtgaatc agcacggaac gccagcgcag cacggccgcg gagcacaccg
    22321 atgagcggtg gactggccgc gcttctcgat gacatcgcgg ccatcgccaa actggcggca
    22381 gcctcgatcg acgacgtcgg agcggctgcg gggcgggcca gcgtcaaggc tgcgggcgtc
    22441 gtcgtcgatg acacggccgt cacccctcgc tatgtgcagg gattcactcc tgaccgcgag
    22501 ttgccgatca tcaagcggat caccatcggg tcgatccgca acaaactggt gttcatcctc
    22561 ccggccatca tgctgctctc gcagttcctg ccgtgggcgc tcacccccct cctcatggtc
    22621 ggcggcacct atctgtgctt cgagggcgcc gagaagatct gggagaaggt cagcggccac
    22681 gacgccgggg aagaagcccc cgccatcgtg cagggcgagg accacgagaa gacgatggtc
    22741 gccggagcga tccgcaccga cttcatcctc tccgccgaga tcatggtcat cgccctcaac
    22801 gaggtcgcca ccgagccctt cctctcgcga ctgatcatcc tcatcgtcgt cgccctcggc
    22861 atcacggtcg gggtctacgg cttcgtcgcc ctcatcgtca agatggacga catcggtctg
    22921 cacctgagcg agcggcagtc cggcgcagcg cgagcgatcg gccgtggcct cgttgccgcc
    22981 atgcccgtcc tactgcgctg gctggccatc atcgggaccg tcgccatgtt gtgggtcggt
    23041 ggccacatcc tgctcgtcgg agccgacacc ctcggctggc acggccccta cgccctggtc
    23101 catcacgcgg aggaggctgt ggccgccgcc gtgtcggtcg gaagcggcgc ccttgcctgg
    23161 ctcaccaaca cgctgggctc ggccatcgcc gggctcatcg tcggcgccgt cgtcgtgctg
    23221 atcatgcacc tgatcccccg caagaaggat gcggccaacg cggcccactg acgtgctcgg
    23281 ccgccggctg gggtggcgac ggtcagcgac gcacgaaggg gttgtcgagc gtcccgatcc
    23341 cctcgatctc gactgagacc cgct
//
LOCUS       DAORPW010000023        23312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_357_length_23312_cov_776.412865, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000023 DAORPW010000000
VERSION     DAORPW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 23312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23312
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_357_length_23312_cov_776.412865"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1742)
                     /gene="ptsP"
                     /locus_tag="PLB86_03550"
     CDS             complement(66..1742)
                     /gene="ptsP"
                     /locus_tag="PLB86_03550"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRC64240.1"
                     /translation="MTDAIRGIGVSPGAAVGPVVQVRPPVRPPDSEPAAADPAAEHVR
                     VKAVFESVAVTLEERASGADETAQQILTATALIARDKGLQKAIGKHLAAGKGPATAVT
                     AAVEEYAAQFEALGGYFAERVTDLKDVRNRAVARLLGKADPGVPDFDVPSVIVAHDLA
                     PAETATLNPSMVRAIVTEAGGRTSHTAILAAQMGIPAVVQFAGATTIPAGTVVAVDGD
                     SGEVTLNPSEDYQRELVGKRERRRSALAGSAGPGRTRDGHPIALLANIGGVDDAVSAA
                     AQDLEGVGLFRTEFVFLSRDSAPTLAEQTEIYTKVFEPFGTRRVVVRTLDAGADKPLA
                     FADLGPEENPALGKRGLRLSALRPDLLDTQLEALGAAAKVTRAEVKVMAPMVATPEEA
                     AWFARRVRENGLPSVGVMIEVPGAALLAKQVLAEVDFGSLGTNDLAQYTMAADRMEGE
                     LSDLLDPWQPAVLKVIGHACDGAKVAGKPMGVCGEAGGDPLLALVLAGLGVGSLSMAP
                     SKVGIVRFSLSLHDLATCHGMATAALDARTAADAREAVIAMAHEDLTALL"
     gene            complement(1748..2017)
                     /locus_tag="PLB86_03555"
     CDS             complement(1748..2017)
                     /locus_tag="PLB86_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRC64241.1"
                     /translation="MATRTVTIASSVGLHARPASLFVQAVNATGLDVEIGRAGEDAID
                     ATSILGVMALGAKHGEEVILTAEGDGAEEALDSLVELLSRDLDAE"
     gene            complement(2131..3645)
                     /locus_tag="PLB86_03560"
     CDS             complement(2131..3645)
                     /locus_tag="PLB86_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HRC64242.1"
                     /translation="MKFVGVTSCPTGIAHTYMAAESLEQAAKDAGHEMIVETQGSAGS
                     DPIPQSVIDAADAVVFAHDLPVKDAARFAGKPTIDVGVKRAISDGPGLIAQAAAAAAD
                     FKANPAKAAAAAAAAPTGGLATKVDSSASLGTQVRQWLMTGVSYMIPFVAAGGILIAL
                     GFMLATFALGKDGPVLITAYDINLPKDVAHSIMDSFNPLSLTWWAALLFRVGVAAFGF
                     LVPALSGFIAYAIADRPGLVPGFVGGAIASTTGAGFLGGLVTGILAGLVARWISRWKV
                     AKGVRGIMPVVVTPLLSTLIVGGLFIVVLGRPIKALMDALTNGLNGLGGGSLVILGLI
                     LGAMMGFDLGGPVNKVAYTFATTGLATVGLATDAVQYKIMAAVMAAGMVAPLALALAT
                     QLRPKLFTEPEQENGKAAWLLGASFISEGAIPFAASDPARIIPASLVGSSITGALCMA
                     FGATSPAPHGGIWVSLLFGNIFGFLIALIVGVVVMAAIVIALKSRDHSLAAANA"
     gene            complement(3642..4091)
                     /locus_tag="PLB86_03565"
     CDS             complement(3642..4091)
                     /locus_tag="PLB86_03565"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HRC64243.1"
                     /translation="MSLISESQVILDLDYPDRHQATRALAETLVADGRCTDLDLFLSD
                     VRKREEQMATGLPGGIGIPHARSGGITTPSLVFGRSRNGIDWGAEDGPATLIFLIAAP
                     DGGGDAHLAILAKLARKLMRKDFRQSLSDAATAAEVVEIVEREVVNA"
     gene            complement(4140..5090)
                     /gene="pfkB"
                     /locus_tag="PLB86_03570"
     CDS             complement(4140..5090)
                     /gene="pfkB"
                     /locus_tag="PLB86_03570"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HRC64244.1"
                     /translation="MIVTLTPNPSIDRTVSVPALEHGEVNRASDSRIDPGGKGINVAR
                     ALATNGTEALAVLPSGGPEGHLLQALLRRSGTAYTAVPIAGTTRMNIAVIEPDGTTTK
                     LNEPGPTLSADEFDALLATVDNHLDPDLRWIVGCGSLTPGMPEGIYADLVRRGHAHGI
                     KVAIDSSGAPFTAAVPAGPDLIKPNHEELEELVGRPLHTLAEVGEAARTLVDGGIETV
                     IVSLGKHGALLVNAVAVAHAIAHVAHPVSTVGAGDSLVAGYLHAVCRGAGPREALATG
                     VAWGAAAVSLPGSRMPGPDDIAAITVTSTDTPDLTLSLTD"
     gene            complement(5222..5983)
                     /locus_tag="PLB86_03575"
     CDS             complement(5222..5983)
                     /locus_tag="PLB86_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRC64245.1"
                     /translation="MYAPERQQAILATARADGRVEVAALAETLKVTPETVRRDLTALE
                     RLGVLRRVHGGALPVERLEVEPTLATRRDQFAEEKLRIAARALDELPREGTVLLDSGS
                     TTLALAEMFPRDRELTVVTNSIAIASALHGHHGVDLYVLGGRIRTRTGAAVGSWATTA
                     LADVSVDVAILGTNGFSVRRGMTTPDQAEAVAKRAMVAAGRRCVVLADSSKAELVHFH
                     RFAELDDVALLITDRRLDEETAERLEASGTDVVRA"
     gene            complement(6139..7197)
                     /locus_tag="PLB86_03580"
     CDS             complement(6139..7197)
                     /locus_tag="PLB86_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HRC64246.1"
                     /translation="MKALRFYAPDDLRLEDVPEPECGPDEVKMRVRNCSTCGTDVKIR
                     HNGHQNLTPPRIIGHEIAGEIVEVGAEVNASYGTQWQVGDRVQVIAAVPCGDCYECSK
                     GWMEVCQNQTSVGYQYDGGFAEFMIVPRQVLKVDGLNRIPDNVGFDEASAAEPFACAI
                     NAQEQLGIEPGDTVVVFGAGPIGCMHIRIARGVHQCGPVYLVDVNAERLAMSADAVHP
                     DEVINAADGDVVARVLELTNGRGADVIITATAANITQEQAIAMAARNGRISFFGGLPK
                     TNPTITCDSNLVHYRQLHIHGANGSAPSHNKKALEYISTGQVPVKDLITRHVALDDVL
                     SAFDIVARGEAIKVTVEP"
     gene            7362..9356
                     /locus_tag="PLB86_03585"
     CDS             7362..9356
                     /locus_tag="PLB86_03585"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022872 -
                     protein-N(PI)-phosphohistidine-mannitol phosphotransferase
                     system transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HRC64247.1"
                     /translation="MSSTQTTEAQRTSARVHVQKFGTFLSNMVMPNIGAFIAWGLITA
                     IFIKSGWLNSGGSGGLAANSWVAQIGGWGDFDGKGIVGPMITYLLPILIGYTGGKIIY
                     DVRGGVVGAIATMGVIAGTSIPMFMGAMVMGPLGGWSIKKLDALWDGKIRPGFEMLVN
                     NFSAGIWGMVLAVFGFFAMGPLIITPFANAAGSVVGWLVDRGLLPLTSLFIEPAKVLF
                     LNNAINHGVLTPLGTNQSLAEGQSILFLLEANPGAGLGLLLAYMVFGKGMAKASAPGA
                     ALIHFVGGIHEIYFPYVLAKPKLIIAMIAGGMTQIFVNVLFHSGLRAPAAPGSILAVY
                     AQTPAASLIGVTLSWILGAAATFFVAAFILKTDKSEDEGDLSRATADMEAMKGKKSIA
                     SATLASGAGGTAVLTKPIRSIVFACDAGMGSSAMGASILRRKIQAAGFGDVTVVNKAI
                     ANLTDDVDLVVSHQDLTPRARQRTGSAVHVSVDNFMGSPAYDEIVEMLGRTNAPGAAA
                     AVVDAAVVEDAPADTAELLTTRCITLSGTARTRDEAITEAGQLLVAAGAVDAAYVASM
                     HDREKSVSTHMGSRLAIPHGTNEAKASIRRSAISFVRYPEGIEWNGKPTTFVIGIAGV
                     GDEHLSLLQRIAHVFLDKSKVAALEAATTPEQVQAILAAG"
     gene            9475..10353
                     /gene="htpX"
                     /locus_tag="PLB86_03590"
     CDS             9475..10353
                     /gene="htpX"
                     /locus_tag="PLB86_03590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HRC64248.1"
                     /translation="MHNHYNGLKTAALFGAIWALLLGLGAVVSGGRYVWIFALIGLGT
                     TFYGYWNSDKLAIRAMRAYPVSEAQAPQMYRIVRELSTKAGKPMPQLFISPTDAPNAF
                     ATGRNPDHAAVCCTEGILNLLDERELRGVLGHELMHVYNRDILTSSVAAAIAGVITSV
                     AQMLMFFGMFGGGGSSDENRPNPLAMLGFALLAPIAASVIQLAISRTREFDADEDGSS
                     LTGDPLALASALRKLEVGVARAPLAPQQQVVNASHMMIANPFRAQDVSRLFSTHPPMH
                     ERVRRLEAMAGGIQGR"
     gene            complement(10411..13368)
                     /locus_tag="PLB86_03595"
     CDS             complement(10411..13368)
                     /locus_tag="PLB86_03595"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HRC64249.1"
                     /translation="MRVGVLGPLLIDGADGETTPRGQRPRDVLAVLLQRRGHVVTPDV
                     LLDLVWGDAAVELTASTVHTVVARLRRALGSDVIETHDAGYRLGRQVRLDSDDFDAAL
                     RRAREATAAGDAAGAVTAYRDAVAVWRGPQAFEGVSDDLVMADRARLGEAHAQTLDEL
                     ALALLESGTTPALDEAWSLARALTREHPLREGSHRTAMLAAVRSGRQAEALDLYRALR
                     ARLRDELGIEPSPPTAALHQRILTQDPTLDPALDVGPTHEQRTGPEPQVTGERPPDQR
                     EMGRLPAPLTSTVGREEDLATVLTLLEQGRRLVTILGPGGVGKSRLLAEVGGRLSADP
                     SLEVVYVDLSGLGATNAADIADAVAVGFRLPVSRDDPISSLVNHLCADRLVALVDEAE
                     WASTAVAEVASHILAGCPGVRLVVTSRVPLDVLGESRVLLAPLACPPEDASGAEARRA
                     PAVRLLEDRLRDHAPDLVIGDEQGADLARLARRVDGLPLALELLAGYAASRTLAELLP
                     LVDAPLDVASPEVGHRPRHRSLRDTLLWSVERLTPSERLVLRRLGVFAGSFDVSAARS
                     VVGGSLDPLTVDAGLRALVREALVQLDRRGPRSRLRLLRTVRDLALEGLDESGETQEV
                     RRRHREWFAARWRGQPPHDELIAEVGASYDDYLEALRQGLAARDACSVADLVIALGRR
                     WSFVEAAGVGVRWFDRVLAADLLDPVDTARVQVLRVGLTLHASWADSRHLIARCQEAL
                     ANDPDWLAQAWQVESIQAYQAGDVERSVTAADQMVTVARRGARWVLPEALATTAVMVA
                     AAGDREGALASADEAWALIGASPTALDFASVVPKIGLALVDADQPQRAYDILSRATTD
                     VSIRLGLRPTFTIVINLGWAALGIDRPAEALSWFARSLNSGSHRGLFLAEECVGAACA
                     LTSLGHPQAAALLDAADELSSRHALSLTPRMAAHRQAARYLQPPSGDRSLSSRTDEDL
                     AAAIGTAAREFAP"
     gene            complement(13425..16628)
                     /locus_tag="PLB86_03600"
     CDS             complement(13425..16628)
                     /locus_tag="PLB86_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRC64250.1"
                     /translation="MTTQAPVSSFDITYQQPGIAGGIRVAAALHRDRLELRLSTGVLA
                     AFFAFPQLGRPHFPEAGNGSDPVMVLGPDRVTVTVVGLPSESAELVRAALADRIALVA
                     SGDPTTVIPLELGPSTPVDGGVGFPLLGRPAERQLYDVALRAGTVGWEVVAPHAVYYR
                     STWTDFGLAHITDTHVARRIDSFRPTLRDLGLTEAAARMCNMNDQFRGFVSFANRLHA
                     AGELDVIVATGDLIDYVHETDDDREGLGNAGFLRDLILGRAPGPDWPTVEELRVPILM
                     TPGNHDYRRHPYHLVFDVKLSGQDLHRVRNFSELALLEREAMALTNTLYFPGATEVPN
                     LGKSAATAMVEIDPTLRAFRQALADPGPHVARLGKHRVVLVDSAHDVGMPDSATDALW
                     ELVKEWWNGSGDEDFMTLIGGSPNCEGVNDEEYAVAVDAIESAPDDGLVVLGLHAPLI
                     NPWNGETPFFLRETQRPALAQQAAWWVQRHTGATSADLMSEHPDWFARPGEGEPAYLK
                     RGTTQDLLDAGVSRGRTDDLLQALAGVGTRRRADVVLAGHTHRYNEISIRVLDDGSLS
                     YFLDFYTANPRAWYPNKVVRVGDVRQAAGGHLDLPTTKTYVEVDEDAIAHAEPHPMPW
                     DATHDWVTFVPPYADPLATSADPRAWWDRHKPLQLQTGALGLWENNQVSFSGLRLLSV
                     RGDVIQRVHFLPRERLDAYRWELSLEQAAAPEPRHQVLTRERTRRFGSPPAASAPLVL
                     TPAAGGNSVVYRDGEGYLVELWDVPGSAGAGRLAGRDVAPAAVGSPSGFVGPDGTAVV
                     LFRGDDRHIHSLYWAGTASAGHDALSQSCEASEAEGDPSGYVLAGITHVFYRTADGHI
                     EELWWPGAEAVSHGHITGYCDEPLAAGDPQGYPVTTTAQNIVLYRGVDGHVHSLYWSD
                     GPTGHDNLSGYCGSPLAAGDPFGYHLPHLDSHQVVYRSADGHLHEIGWAGAAPASAWD
                     VVGAAGAPAAAADPACWFVPANGTKHISYAGVDGHVHDLAWPAGTATPTWTDLTLSAL
                     APPAAAELVTGWVEPGSATCRVAFRGTDGHLHEIRWG"
     gene            complement(16792..19092)
                     /locus_tag="PLB86_03605"
     CDS             complement(16792..19092)
                     /locus_tag="PLB86_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRC64251.1"
                     /translation="MIDTFITTVMRRARAILITSLVILVAAAALGVGAISRLQSGGFD
                     DPSAESAQAARALAEKLGRPTANFLLLVTAPSGATVDDAVVADVGRAAVSRLDAEPGV
                     EVVADFWSAPAGAAAALRGAGGRTALVVAHIDGDEDDYRERIATLQPAYTTTSPEGVT
                     VQTGGAAQTVTDITLQVKKDFALAEALAIPATLLLLVVVFGSFIAGLLPMLVGAYAMV
                     GTLAVLRILTAVTDVSIFSLNLTTALGLGLGIDYALLVVNRFREELQSGDDVEGALRV
                     TMHTAGRTVVYSAITVAVALGALLAFPMYFLRSFAYAGIAVTLLTAGVSLVVLPAALR
                     LLGHRAAYRRFGKPTRYAAAGLWSRVATVVMRRPVLTAAPVVVLLGVLAAPLAGVSFG
                     LPDDRSIPVSHSQGRAVAEVLRADFTSRESEAITVVFPDGAPERTDRPTLAAYGASLS
                     VLPSVSRVDTPVGTYVRGARVSGPRPDLLSDSAAAVLVIPDVEVYSQAAQDLVTAIRR
                     ADAPAAPLVGGPTARFVDVNASIASRLPLVGALIALTTFLVIFFFTGSIVLPVKALLT
                     NAATIAAVLGVMVWVFQEGHGSGLLGFTPTPLSVSIPPLMFCLAFGLSMDYEVFLLGR
                     IKEARDRGLDDTAAVASGLGSTGRIVTAAAALMSVTFLAFSTSKVSFIQMLGLGCALA
                     ILLDATLVRGVLVPALMRLMGRWNWWAPAPLVRLHERIAPAPEGLPRPLGLARTGTDA
                     SRRNAGDARGEHHPNTAGEAMSGTVR"
     gene            complement(19139..19684)
                     /locus_tag="PLB86_03610"
     CDS             complement(19139..19684)
                     /locus_tag="PLB86_03610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC64252.1"
                     /translation="MPWLTPALPAFSPATHPYLTIRPLAPLEAGPLQAVFQGLSAKSR
                     QLRYHTPVSVLRPAMLRHLTDVDPGRHVAVVATFGGRPVGIGRWLRDPLRPTEAELAV
                     EVIDRCHGLGIGTALAAEAARLAVHAGVDTLVAHIDSANTAARGWARALGAAPHPDDP
                     EQVRIAAAALAPDIPRHHPAA"
     gene            19805..>21123
                     /locus_tag="PLB86_03615"
     CDS             19805..>21123
                     /locus_tag="PLB86_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRC64253.1"
                     /translation="MPSLTRAEATRRAAQLAVQGYAVALDLDVGDERFASLTTVTFQA
                     LADGVQTWVDVKATDVHEVLLDGIPVDLAGWHDGRLALPALAAGAHTLSVDATMAYRR
                     DGSGLHRAVDPADGEAYVYVQSFLDYAPAVFACFDQPDLKASISLRVHAPTHWTIVGN
                     GAATRAGDGEWELAATAPISTYLFTVCGGPFVSVRDSHDGIPLGLHARASMRAELERW
                     APQIFEVTKQSFDAYHALFGIRYPFGDYHQVFVPDFNAVAMENPGCVTLRDALLYRGA
                     ATPDEVLRRSRTVCHEMAHMWFGDLVTMKWWDDLWLNESFAEYLAHRVLTGATEFADA
                     WVDFGILRKPWGYAAERAPSAHPVAGAPALTAEDALSDFDGISYAKGATALRQLIAFV
                     GDEVFVGGVSNYLRDKAFGNGTFAEFLAAIEASAGRDLGDWARDWLLT"
     assembly_gap    21124..21223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21224..22348
                     /locus_tag="PLB86_03620"
     CDS             <21224..22348
                     /locus_tag="PLB86_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HRC64254.1"
                     /translation="DGPVLTRRAPAAYPASRPHVLDVGVYAASAPPEVTRVLADRERT
                     ALPDLRVAPGDRLVLPNCSDLTWASIDFDEATLAALPARLGELADPLARSVCWQSLDD
                     GVARGVVDPRLFLDAIARSWPRETHPALFANVADDALVVLSRFVPDDLRPGLRAELAE
                     TARTVIERESDGSPRATIAARLVARASTHEGLLRTWLDAGPSWLQGDTDFRWLVVGNL
                     AGLGRLREEELAAAEAADPTVSGRLAGLLARASVPTVAAKTWAFEQLVDPASGHTNHA
                     LVELARGLWRSPDRGLVRPFVEPFLDAIPRMTAWVGDDALTKVVRFGFPFVVEARTIE
                     LVDAALARNDLTPGVRRAFVEWSWPVREALASRSRWFPTG"
     gene            22479..>23312
                     /locus_tag="PLB86_03625"
     CDS             22479..>23312
                     /locus_tag="PLB86_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRC64255.1"
                     /translation="MTPAQHAAAVRALSLSYEHLDPDVPVRLAKRTTNLFRPRAAAMG
                     PGLDVSALTGVIDVDPVAGTATVQGMATYESVVDATLAAGVMPLVVPQLRTITVGGAV
                     TGLGIEASSFRNGLPHESVRSMDILIGTGEIVTAAPTGPHADLFRTFPNSYGSLGYAV
                     SLTIDVEPVAPFVRLRHLPFDSLQSLTEAVGQIVAARAWDGEPVDFLDGVVFARNAAY
                     LTLGRFAQSLDAAETPSDYAGQRIFYRSIRERSTDVLTVRDFLWRWDTDWFWCSRAFG
                     VQ"
BASE COUNT         3452 a         7655 c         8561 g         3544 t
ORIGIN      
        1 aacactgagg cgttggccgc tttgcgcggc caacgcctca ggtccctcca cccgctccca
       61 gtggcttaga gcaacgcggt caagtcctcg tgggccatcg cgatcacggc ctcccgggca
      121 tcggctgcgg tgcgggcgtc cagcgctgcc gtggccatgc catggcaggt cgccaggtcg
      181 tgcagcgaca acgagaaccg gacgatgccg accttggacg gagccatcga caggctgccg
      241 acaccgagcc cggccagcac gagagccagc agcgggtcgc cgccggcctc gccgcagaca
      301 cccatcggtt tgccggcgac cttggctccg tcgcaggcgt gcccgatgac tttgaggacg
      361 gccggctgcc aggggtcaag caggtcggac agctcgccct ccatccggtc ggcagccatc
      421 gtgtattgcg cgaggtcgtt ggtgccgagc gagccgaagt cgacctccgc gaggacctgc
      481 ttggccaaca gggccgcacc ggggacctcg atcatgacgc cgacactggg caggccgttc
      541 tcgcggaccc ggcgggcgaa ccaggccgcc tcctcggggg tggcgaccat gggggccatg
      601 accttgacct cggcacgggt gaccttcgcc gccgctccga gagcctcgag ttgggtgtcg
      661 agcaggtcgg ggcgcagcgc cgacaaacgc agtccgcgct tgcccagggc cgggttctcc
      721 tccggcccga ggtcggcgaa ggccaacggc ttgtcggccc cggcatcgag ggttcgcacg
      781 acgacccggc gggtgccgaa cggctcgaag accttggtgt agatctcggt ctgctcggcg
      841 agcgtgggtg cggagtcgcg gctgaggaag acgaactcgg tgcggaacag cccgacccct
      901 tcaaggtcct gagccgccgc gctcaccgcg tcgtccacgc caccgatgtt ggccagcagg
      961 gcgatcgggt gtccgtcacg ggtgcgcccg gggccggctg agccggccag ggctgaacgg
     1021 cgtcgctcac gcttgccgac caactcccgc tggtagtcct cgctcgggtt cagcgtcacc
     1081 tcgccggagt cgccgtcgac ggcaaccacc gtgccggccg ggatcgtggt cgcaccggcg
     1141 aactgcacga ccgcggggat acccatctgc gcggcgagga tcgcggtgtg gctggtgcgc
     1201 ccgccggcct cggtgacgat cgcgcggacc atgctcgggt tgagcgtcgc ggtctcggcc
     1261 ggggccaggt cgtgggcgac gatgacgctg gggacgtcga agtccgggac acccgggtcc
     1321 gccttgccca gcagtcgcgc gacggcccgg ttacggacgt ccttgaggtc ggtgacccgc
     1381 tcggcgaagt agccgccgag cgcttcgaac tgcgcggcat actcctcgac ggcggcggtg
     1441 accgcggtgg cggggccctt gccagcggcg aggtgcttgc cgatcgcctt ctgcaaaccc
     1501 ttgtcgcggg cgatgagggc agtggccgtg aggatctgct gggcggtctc gtcagcgccg
     1561 ctggcgcgct cttcaagcgt cacggcgacc gactcgaaga cggccttgac gcgcacgtgc
     1621 tcagcggcgg ggtcggctgc ggcgggctcg gagtccggcg gtcggacggg cggtcgcacc
     1681 tggaccaccg gcccgacggc cgctccgggg ctgacgccga tgccgcggat ggcgtcggtc
     1741 acgtcggtca ctccgcgtcg aggtcgcggg agagcagttc gacgagcgag tcgagggctt
     1801 cctcggcgcc gtcgccttcg gccgtgagga tgacctcctc gccgtgcttg gcgcccaggg
     1861 ccatgacgcc gaggatgctc gtggcgtcga tggcgtcctc gccggcgcgg ccgatctcga
     1921 cgtccagacc agtggcgttc actgcctgga cgaacaacga ggcgggtcga gcgtgcaggc
     1981 cgacggagga ggcgatggtg acggtgcggg tggccatgag gtgtcctttc cgggggtgga
     2041 accactgcgg gtgggtatgc cgtgcggcgc ggctcgaggc acgcgccgca cgacatgcgt
     2101 gtggctaggg atgaacctag cggtggaggg tcaggcgttc gcggcggcca ggctgtggtc
     2161 gcgtgacttc agcgcgatca cgatggcggc catgacgacc acgccgacga tgagggcgat
     2221 gaggaacccg aagatgttgc cgaacagcag cgacacccag atgccaccgt ggggagccgg
     2281 cgaggtcgcg ccgaaggcca tgcagagcgc acccgtgatg gacgagccga cgagcgaggc
     2341 cgggatgatg cgggccgggt cggaggcggc gaatgggatg gcaccctcgg agatgaacga
     2401 ggctcccaac aaccaggcag ccttgccgtt ctcctgctcg ggctccgtga agagcttcgg
     2461 gcgcagttgc gtggccaggg ccagagcgag cggggcgacc attccggcgg ccatgacggc
     2521 ggccatgatc ttgtactgca ccgcgtccgt ggccagaccg accgtggcga gcccggtggt
     2581 agcgaaggtg taggccacct tgttgaccgg gccaccgagg tcgaagccca tcatcgcgcc
     2641 gaggatgagg ccgaggatca cgagggagcc gccgccgagg ccgttgaggc cgttggtcag
     2701 ggcgtccatg agggccttga tcggccggcc gaggacgacg atgaacagcc caccgacgat
     2761 gagggtcgac agcagcgggg tgacgacgac cggcatgatg ccgcggacgc ccttggcgac
     2821 cttccagcgc gagatccagc gggcgacgag gccggcgagg atgccggtga cgaggccgcc
     2881 gaggaagccg gcgccggtcg tgctggcgat ggcgccaccg acgaacccgg ggaccaggcc
     2941 ggggcggtcg gcgatcgcgt aggcgatgaa gccggacagg gccgggacga ggaagccgaa
     3001 ggccgcaaca ccgacgcgga agagcagcgc cgcccaccag gtgaggctca gcgggttgaa
     3061 gctgtccatg atgctgtgcg cgacatcctt gggcaggttg atgtcgtatg ccgtgatgag
     3121 gacagggccg tccttgccga gggcgaaggt cgcgagcatg aagccgaggg cgatgaggat
     3181 gccaccggcg gcgacgaacg ggatcatgta ggagacaccg gtcatgagcc actggcggac
     3241 ctgggtgccg aggctggccg acgagtcgac cttggtcgcg aggccaccgg tcggggccgc
     3301 ggcggctgcg gcagcggcct tggcggggtt ggccttgaag tccgctgcgg cagccgcggc
     3361 ttgggcgatg aggcccgggc cgtcgctgat cgcgcgcttg acgcccacgt cgatggtcgg
     3421 cttgccggcg aagcgggcag cgtccttgac cgggaggtcg tgggcgaaga ccacggcgtc
     3481 agcggcgtcg atgaccgact gggggatcgg gtcagatccg gccgagcctt gggtctcgac
     3541 gatcatctcg tggccggcgt ccttggctgc ctgctccaag gactcggcgg ccatgtaggt
     3601 gtgtgcgatg cccgtggggc acgaggtcac tccgacgaac ttcatgcgtt cacaacctct
     3661 cgttcgacga tctcgacgac ctcggccgcg gtggctgcgt cgctcaggga ctggcggaaa
     3721 tccttacgca tgagcttgcg cgcgagcttg gcgaggatgg ccaagtgcgc atccccaccg
     3781 ccatcgggtg cagcgatgag gaagatgagg gtggccgggc cgtcctcggc gccccagtcg
     3841 atgccgttgc gcgagcgccc gaagaccaac gacggggtgg tgatcccgcc tgagcgggcg
     3901 tgcgggatcc cgatgccgcc gggcagcccg gtcgccatct gctcttcacg cttgcggacg
     3961 tcggagagga acagatcgag gtcggtgcat cggccgtcgg ccacgagggt ctcggccagg
     4021 gcgcgcgtcg cctggtgccg gtcgggatag tccaggtcga ggatgacctg gctctcgctg
     4081 atgagggaca tggtgaggga ctccttagat gtgaatcgtc gggctcggca aaactggggt
     4141 caatcggtga gcgacaaggt gaggtcggga gtatcggtgc tggtcaccgt aatggcggcg
     4201 atgtcgtccg ggccaggcat ccggctgccg ggaagactga cggcagcggc gccccaggcg
     4261 accccggtgg cgagcgcttc acgggggccg gcgccgcggc ataccgcgtg gaggtaaccg
     4321 gccacgagcg agtctccagc gccgacggtc gacaccgggt gggcgacgtg cgcgatcgcg
     4381 tgggcgacgg cgacggcgtt gaccagcaaa gcgccgtgct taccgaggct gacgatgacc
     4441 gtctcgatgc cgccgtcgac cagcgtgcgc gcggcctcac cgacctcggc gagggtgtgc
     4501 agcgggcgac cgacgagctc ttcgagctcc tcgtggttgg gcttgatgag gtcggggccg
     4561 gcgggcacgg cggccgtgaa cggcgccccg gaggagtcga tggcgacctt gatgccatgc
     4621 gcgtggccgc ggcggacgag gtcggcatag atgccttcgg gcatccccgg ggtgaggctg
     4681 ccgcagccga cgatccagcg caggtcaggg tcgaggtggt tgtcgaccgt cgcgaggagg
     4741 gcgtcgaact cgtcggcgga gagggtcgga ccgggctcgt tgagcttggt ggtcgtgccg
     4801 tcgggctcga tgaccgcgat gttcatccga gtcgtcccgg cgatggggac cgcggtgtat
     4861 gccgtgcccg agcggcgcag gagggcctgc agcaggtgcc cctcgggtcc gccggaggga
     4921 agcacggcga gggcctcggt gccgttggtg gccagcgcgc gggcgacgtt gatgcccttg
     4981 ccgcccgggt cgatccggct gtcggacgcc cggttgactt cgccgtgctc gagagccggc
     5041 accgagacgg tgcggtcgat gctcgggttg ggggtgaggg tgacgatcat cctggcctcc
     5101 tgagggtgcg caagcgcttg cacaaatatt cccacacaaa caaagcgatg taaatagtct
     5161 tgagccggat tttgtgtccg agttgctcgc cagcgtggtt gctgaggcgt gccggcgccg
     5221 ttcaagcccg gaccacgtcc gtcccgctgg cctcgagccg ctcggcggtc tcctcgtcca
     5281 ggcggcggtc ggtgatgagc agcgcgacat cgtcgagctc ggcgaaccga tggaagtgga
     5341 cgagttcggc cttggacgag tcggcgagca cgacacatcg ccgcccggcc gcgaccatgg
     5401 cgcgcttggc cacggcctcg gcctggtcgg gagtggtcat cccgcgccgg accgagaagc
     5461 cattggttcc gagaatcgcg acgtcgacgc tgacgtcggc gagggccgtc gtggcccagg
     5521 acccgaccgc cgcgcccgtg cgcgtgcgga tccggcctcc caagacatag aggtccaccc
     5581 cgtgatggcc gtgcagagcc gaggcaatgg cgatcgagtt ggtgacgacc gtcaactcgc
     5641 gatcacgcgg aaacatctcg gcgagggcca gcgtcgtcga tcccgagtcg agcaggacgg
     5701 tgccttcgcg cgggagctcg tccagggcgc gggcggcgat ccgcagtttc tcctcggcga
     5761 actggtcgcg tcgggtggcc agcgttggtt cgacttcgag ccgttcgacg ggcagggctc
     5821 caccatggac gcggcgcaga acgccgagac gctcgagcgc cgtgaggtcg cggcgcacgg
     5881 tctcgggagt gaccttgagg gtctcggcca gggccgcgac ctccactcgg ccgtcggctc
     5941 tcgccgtggc caggatggcc tgttgacgtt ctggggcata caacggggca cgctcctcgt
     6001 ctgggctcgg cgccgttgcc gggcctgggg tcggtgatcg gtcctccggg caagccatgt
     6061 gtgggattct gcccgaaccg gcccgttcgg atctaccggg gatctgcctt cgtggggccg
     6121 acccccggcc gttccgcgtc agggctcgac cgtgaccttg atcgcctcgc cgcgagcgac
     6181 gatgtcgaag gcactcagga cgtcgtcgag ggcgacatgc cgggtgatga ggtccttgac
     6241 ggggacctgg ccggtcgaga tgtactccag ggccttcttg ttgtggctgg gggccgagcc
     6301 gttggcgccg tggatgtgca actggcggta gtgaacgagg ttcgagtcgc aggtgatcgt
     6361 cgggttggtc ttgggcagcc caccgaagaa cgagatccgc ccgttgcgag cagccatggc
     6421 gatcgcctgc tcctgggtga tgttggcggc cgtcgccgtg atgatgacgt ctgccccgcg
     6481 gccgttggtc agctccaaca cgcgggccac gacatcgccg tcggccgcgt tgatgacctc
     6541 gtcggggtgg acggcgtcgg ccgacatggc caggcgctcg gcgttgacat cgacgagata
     6601 gaccggcccg cactggtgca cgccgcgggc gatccggatg tgcatgcagc cgatcgggcc
     6661 ggcgccgaag accacgacgg tgtcgccggg ctcgatgccc aactgctcct gggcgttgat
     6721 cgcgcaggcg aacggctcgg cggccgaggc ttcgtcgaag ccgacgttgt ccgggatccg
     6781 gttgagtccg tcgaccttga ggacctggcg ggggacgatc atgaactccg cgaagccgcc
     6841 gtcgtactgg tacccgacgg acgtctggtt ctggcagacc tccatccaac ccttcgaaca
     6901 ctcgtagcag tcgccgcacg gcaccgcggc gatgacctgg acccggtcgc cgacctgcca
     6961 ctgcgtgcca taactcgcgt tgacctcggc gccgacctcg acgatctcgc cggcgatctc
     7021 gtgaccgatg atgcgcggcg gggtgaggtt ctggtgcccg ttgtggcgga tcttcacgtc
     7081 cgtgccacag gtggagcagt tgcggacccg catcttgacc tcgtccgggc cacactcggg
     7141 ctcggggacg tcctccaggc gcaggtcgtc gggggcgtag aagcgcaggg ccttcatcga
     7201 tgctcctcca ttggggctaa tcgggccgct ccgcgacccc aatcatgtgt gtttgtgcct
     7261 gaatcatggc atggaacctt gacacggtca agaacgggcc tatataaagg caaacacaga
     7321 cccgcggtat gtcgtgggtc acagcgaagg gagctggcaa ggtgtcgtca acgcagacca
     7381 ccgaggccca acgaacgtcg gcccgtgtcc acgttcagaa gttcgggacg ttcctgtcca
     7441 acatggtcat gcccaacatc ggtgcgttca tcgcctgggg actcatcact gcgatattca
     7501 tcaagtccgg gtggctcaac tccggtggca gcggcgggct cgccgccaac agctgggtcg
     7561 cccagatcgg cgggtggggc gacttcgacg gcaagggcat cgtcggcccg atgatcacct
     7621 acctgttgcc gatcctcatc ggctacaccg gcggcaagat catctacgac gtccgcgggg
     7681 gcgtcgtcgg tgccatcgcg acaatgggtg tcatcgccgg gacgagcatc ccgatgttca
     7741 tgggtgccat ggtcatgggt ccgctcggtg gctggtcgat caagaagctt gacgccctgt
     7801 gggacggcaa gatccggccg ggcttcgaga tgctcgtcaa caacttctcg gcgggcatct
     7861 gggggatggt ccttgcggtc ttcggcttct tcgccatggg gccgctcatc atcacgccgt
     7921 tcgcgaacgc cgcaggaagc gtcgtggggt ggctcgttga ccgtggcctg ctgccgttga
     7981 cgtcgctgtt catcgagccg gccaaggtgc tgttcctcaa caacgcgatc aaccatggtg
     8041 tcctcacccc gctcggcacc aaccagagcc tcgccgaggg acagtcgatc ctcttcctcc
     8101 tcgaagccaa ccctggcgcg ggcctcgggc tactgctcgc ctacatggtg ttcggcaagg
     8161 gcatggccaa ggcctcggcc ccgggcgctg ccctcatcca cttcgtcggc ggcatccacg
     8221 agatctactt cccctacgtc ctggccaagc cgaagttgat catcgcgatg atcgccggtg
     8281 gtatgaccca gatcttcgtc aacgtcctct tccactccgg cctgcgggcg ccggccgcac
     8341 ctggctcgat cctcgccgtc tacgcgcaga cccccgccgc cagcctgatc ggtgtcacgc
     8401 tctcgtggat cctcggcgct gcggcgacct tcttcgtcgc cgcgttcatc ctgaagaccg
     8461 acaagtccga ggacgagggt gacctcagcc gggcgacggc ggacatggag gcgatgaagg
     8521 gcaagaagtc gatcgcctcg gcgaccctcg ccagtggtgc gggtggcacg gccgtgctca
     8581 ccaagcccat tcgctcgatc gtcttcgcct gcgacgcggg catgggctcc tcggccatgg
     8641 gtgcctcgat cctgcggcgc aagatccagg cagcgggctt cggtgatgtg accgtcgtca
     8701 acaaggcgat tgcgaacctc accgacgatg tcgacctggt ggtcagccac caggacctca
     8761 ccccgcgggc acgccagcgg acgggctcgg cggtccacgt gtcggtcgac aacttcatgg
     8821 ggtcgccggc ctacgacgag atcgtcgaga tgctgggccg gaccaacgcg cctggggcag
     8881 cggcagccgt tgtggatgcg gccgttgtcg aggacgcccc ggccgacacc gccgagttgc
     8941 tcaccactcg ctgcatcacc ttgtcgggca cagcccgcac gcgtgacgag gcgatcaccg
     9001 aggccggcca gttgctggtg gccgcggggg ccgtcgacgc ggcatacgtc gcctccatgc
     9061 acgaccgcga gaagtccgtg tccacccaca tgggcagccg tctggcgatc ccgcacggga
     9121 ccaacgaggc gaaggcgtcg atccggcgct cggcaatctc gttcgtgcgt taccccgagg
     9181 ggatcgagtg gaacggcaag ccgacgacct tcgtcatcgg catcgccggt gtcggggacg
     9241 agcacctctc gctgctgcag cggatcgcgc acgtgttcct cgacaagtcc aaggtggctg
     9301 cacttgaggc cgccaccacg ccggagcagg tccaggccat cctcgcggcc ggctgatcga
     9361 ccgactccgt gggtcacggt ccgcgcgccc ttgggggggg cgcgcgggcc gtccccgttt
     9421 tgggggtcgc cgtcggtggg aacaacggtg cgtcgctgcc tcgttgagcc agctatgcac
     9481 aaccactaca acggtctcaa gaccgctgct ctcttcggcg ccatctgggc gctgctgctc
     9541 gggctcggcg cagtggtgtc cggggggcgc tacgtctgga tcttcgcgct catcggcctc
     9601 ggcacgacgt tctatgggta ttggaacagc gacaaactgg cgatccgcgc gatgcgggcc
     9661 tacccggtga gcgaggccca ggccccgcag atgtatcgga tcgtccgcga gttgtcgacc
     9721 aaggccggca agccgatgcc ccagctgttc atcagcccga ccgatgcgcc caatgccttt
     9781 gccaccggcc gcaatccgga tcatgcagcc gtctgctgca ccgaggggat cctgaacctg
     9841 ctcgacgaac gcgagctgcg cggggtgctc gggcacgagc tcatgcacgt ctacaaccgc
     9901 gacatcctca cctcgtcggt ggccgccgcc atcgccggtg tcatcacctc ggtcgcccag
     9961 atgctcatgt tcttcggcat gttcggtggc ggaggcagct cagacgagaa ccgcccgaac
    10021 cccctcgcca tgctcggctt cgccctgctg gctccgatcg ccgcctcggt catccagctc
    10081 gcgatcagcc ggacccggga gttcgacgcc gacgaggatg gctcgagcct caccggtgat
    10141 ccgctcgccc tcgcctcagc gctgcgcaag ctcgaggtgg gtgtcgctcg ggcgccgctc
    10201 gccccgcaac agcaggtcgt caacgccagc cacatgatga tcgccaaccc gttccgcgcc
    10261 caggacgtct cgcggctctt ctccacccac ccgccgatgc acgagcgggt ccgtcgcctc
    10321 gaggcgatgg ccggcgggat ccagggacgc tgaacccacc tgccgcgcaa cggcataagc
    10381 cctcggtatg ccgtgcggct gggtccgcgg tcagggtgcg aactccctgg ctgccgtacc
    10441 gatcgcggcg gccaggtcct cgtcggtgcg gctgctgagc gaccggtcgc ctgagggcgg
    10501 ctgcaggtat cgggcggctt ggcgatgggc ggccatgcgc ggcgtgaggc tgagcgcatg
    10561 ccgggaggac aactcgtcgg cagcatcgag cagcgccgca gcctgggggt ggccaagaga
    10621 tgtcaacgca cacgccgccc cgacgcactc ttcggccaag aacaaccctc gatgtgaccc
    10681 tgagttcagc gaccgcgcga accagctcaa cgcctccgct ggccggtcga tgcccagcgc
    10741 ggcccacccc aggttgatca cgatggtgaa cgtgggccgc aggcctagtc ggatgctgac
    10801 gtccgtggtg gctctgctga ggatgtcgta ggcccgttgc ggttgatcgg cgtcgaccag
    10861 tgccaggccg atcttcggaa cgaccgaggc gaaatccagg gccgtcggtg aggccccgat
    10921 cagggcccaa gcctcgtcgg ccgaggcgag cgccccctcc cgatcgccag ccgccgcaac
    10981 catgacggca gtcgtggcga gagcctcagg caacacccac ctggcccccc ttcgcgcgac
    11041 cgtgaccatc tgatccgctg ccgtgacgct tcgctcgaca tcacctgcct gataggcctg
    11101 gatcgactcc acctgccacg cctgagcgag ccaatcgggg tcgttcgcca gagcctcctg
    11161 gcacctcgcg atgagatgcc tggagtcggc ccacgacgcg tgcaaggtca ggccgacccg
    11221 cagcacctgc acgcgggccg tgtccaccgg atcgagcagg tcagcggcca acacccggtc
    11281 aaaccaccgc acgccgactc cggcagcctc gacgaaggac cagcgtcggc ccaacgcgat
    11341 caccaggtcg gcgaccgagc aggcgtcgcg ggccgccagc ccctggcgca gcgcttcgag
    11401 gtagtcgtcg taactcgcgc ccacctcggc aatcagttcg tcgtgcggcg gctgtccgcg
    11461 ccaccgtgcc gcgaaccact ctcgatggcg gcgccgcacc tcctgggtct cgccgctctc
    11521 gtcgagcccc tcaagggcga gatcgcggac ggtgcgcagc agccgcagtc gagatcgcgg
    11581 gccgcgccga tcgagttgga cgagggcttc gcgcacgagt gcgcgcaggc ccgcatcgac
    11641 cgtgagcgga tccagcgagc caccgacgac ggaccgagcg gcagaaacgt cgaacgaccc
    11701 cgcgaagacg cccaggcgtc gcaggaccag gcgttcgctc ggggtcagcc gctcgacgct
    11761 ccacagcagg gtgtcgcgca gacttcggtg gcgtggccgg tgacccactt cggggctggc
    11821 cacgtccagg ggtgcatcga cgaggggcag caactccgcg agggtccgcg acgcggcata
    11881 cccggcgagc agttcgagcg cgagggggag cccgtcgacg cggcgagcca accgcgcgag
    11941 gtcagcgccc tgctcatcgc cgatgacgag atccggcgcg tggtcgcgca ggcgatcctc
    12001 gaggagccgg acggccgggg cgcgccgggc ctcggcgccg gaggcgtcct ccggagggca
    12061 ggccagcggg gcgagcagca cccggctctc cccgaggacg tccaggggga cgcgcgaggt
    12121 gacgacgagc cggacgcccg ggcagcccgc gaggatgtgc gaggccactt cggcgaccgc
    12181 tgtcgaggcc cactccgcct cgtcgacgag cgcgacgagg cggtctgcgc acagatggtt
    12241 gaccaggctg gagatcgggt cgtcgcgaga caccgggagc cggaagccca ctgccacggc
    12301 gtcggcgatg tcggcggcgt tcgtggcgcc gaggcccgac aggtcgacat agacgacctc
    12361 cagcgagggg tccgcgctca gccgcccgcc gacctcggca agcaggcgac tcttacccac
    12421 cccgcccggc ccaaggatcg tcaccaatcg acgcccctgc tccagcaggg tgagcaccgt
    12481 ggcgaggtcc tcctccctgc ccaccgtcga ggtgagcggc gccgggagcc gacccatctc
    12541 ccgttggtcc ggcggccgct cgcccgtgac ctgcggctcc gggccggtcc gctgttcgtg
    12601 ggtcgggcca acgtcgaggg cggggtcgag ggtggggtcc tgcgtgagga tgcgttggtg
    12661 cagcgccgca gtcggtgggc tcggctcgat ccccagctca tcacgcagcc gggcccgcaa
    12721 ggcccgatag aggtccagcg cctcggcttg gcggccgctg cggacggccg cgagcatggc
    12781 cgtccgatgg ctcccctcgc gcaggggatg ctcccgggtc agcgccctgg ccagggacca
    12841 ggcctcgtcc agggcagggg tcgttcccga ttccaacagc gcgagcgcga gctcgtcgag
    12901 ggtctgggcg tgggcctcgc cgagccgggc tcgatcggcc atgaccaggt cgtcgctgac
    12961 cccttcgaag gcctgcggtc cgcgccacac ggcaacggca tcccggtatg ccgtgaccgc
    13021 cccggccgcg tcgcccgccg cggtggcctc gcgggcccgg cgcagggccg cgtcgaagtc
    13081 gtccgaatcc agccgcacct gccggccgag gcggtagccg gcgtcgtggg tctcgatgac
    13141 gtcactgccc aaggcccgcc gcaaccgggc gacgacggta tggaccgtcg atgcggtgag
    13201 ctcgacagcg gcgtcgcccc acaccagatc gaggagcacg tcaggggtga cgacatggcc
    13261 gcggcgctgg agcagcacgg cgagcacgtc ccgcggccgt tggccgcggg gcgtggtctc
    13321 accgtccgct ccgtcgatca gcagcggccc gagaacgccg acccgcatcc gcccatgcaa
    13381 tcacaggacg gctgcgtgtc gcgcccgaat tgtcgggacg ttgctcagcc ccaacggatc
    13441 tcgtggaggt gcccgtcggt gccgcggaag gccacccggc aggtcgccga ccctggctcg
    13501 acccaaccgg tcacgagctc ggcggccgcc ggcggggcga gggcgctgag agtgagatcg
    13561 gtccaggtcg gtgtcgcggt cccagccggc caggcgaggt cgtggacgtg accgtcgaca
    13621 ccggcatacg agatgtgctt ggtgccgttt gcggggacga accagcaggc cgggtcagcc
    13681 gcagcagcgg gtgcgccggc agctccgacg acgtcccagg cgctcgccgg tgcggcgccg
    13741 gcccagccga tctcatgcag gtgcccgtcc gcggagcggt agacgacctg gtgggagtcc
    13801 aggtggggca ggtgatagcc gaacgggtca cccgcggcga gcggcgatcc gcaatagccc
    13861 gacaggttgt cgtgccccgt ggggccatcc gaccagtaga gcgagtggac gtgcccgtcg
    13921 acgccccgat agaggacgat gttctgggcc gtcgtcgtga ccgggtatcc ctggggatct
    13981 cccgcggcga ggggttcgtc gcaatacccg gtgatgtggc cgtggctgac ggcctcggcc
    14041 cccggccacc acaactcctc gatgtgcccg tcggcggtgc gatagaagac gtgggtgatg
    14101 ccggccagga cgtagccgct ggggtcgcct tcggcctcgg atgcctcgca cgactgcgac
    14161 agcgcgtcgt gtccggcgct cgccgtcccc gcccagtaga gcgagtggat gtggcggtcg
    14221 tcgccgcgga acagcacgac cgcggtcccg tcggggccga cgaatcccga cggcgagccg
    14281 accgcggcag gagcgacgtc gcgcccggcg agccgcccgg cccccgcgct gccggggacg
    14341 tcccacaact cgacgaggta tccctcaccg tcgcgataga ccacgctgtt cccgcccgcc
    14401 gctggggtga ggaccagggg agcgctcgcg gccggcggcg acccgaaccg cctggtccgc
    14461 tcccgggtga gcacctggtg gcgcggctcc ggggccgccg cctgctcgag cgacaactcc
    14521 cagcggtagg cgtccaggcg ttctcggggg aggaagtgca cccgctggat gacgtccccg
    14581 cgcacgctga gcagccgcag cccgctgaag ctcacctggt tgttctccca cagcccgagg
    14641 gcaccggtct gcagttgcag cggcttgtgc cggtcccacc acgcgcgcgg gtcggccgac
    14701 gtcgccagcg gatcggcata cggcgggacg aaggtgaccc agtcgtgggt ggcgtcccag
    14761 ggcatcgggt gcggctcggc gtgcgcgatg gcgtcctcgt cgacctcgac ataggtcttg
    14821 gtggtcggga ggtcgaggtg gccgcccgcc gcttggcgaa cgtccccgac ccgcacgacc
    14881 ttgttgggat accaggcgcg tgggttggcc gtgtagaagt cgaggaagta gctgaggctg
    14941 ccgtcatcga ggacccggat cgagatctcg ttgtagcgat gggtgtgtcc ggcgagcacg
    15001 acgtcggccc gccgccgggt cccgacaccg gcgagcgctt gcaacaggtc gtcggttcgt
    15061 ccccgcgaga cccctgcatc caggaggtcc tgggtggtcc cgcgcttgag gtatgccggt
    15121 tcgccctcac cggggcgcgc gaaccagtcg gggtgctcgc tcatgaggtc ggccgaggtt
    15181 gctccggtat gccgttgcac ccaccacgct gcttgctggg cgagggcggg gcgctgggtc
    15241 tcgcgcagga aaaagggcgt ctccccgttc catggattga tgaggggggc gtgcagcccg
    15301 aggacgacga gcccgtcgtc gggggccgac tcgatggcgt cgacggcgac ggcatactcc
    15361 tcgtcgttga ccccctcgca gttgggggag ccgccgatga gcgtcatgaa gtcttcgtcg
    15421 cccgagccgt tccaccactc cttgacgagc tcccacagcg cgtctgtggc cgagtcgggc
    15481 atgccgacgt cgtgtgcgct gtcgacgagg acgacgcggt gcttgcccag tcgagcgacg
    15541 tgtgggccgg ggtcggccaa tgcctggcgg aaggcgcgca gggtcgggtc gatctccacc
    15601 atcgccgtgg cggcggactt gccgaggttg ggcacctccg ttgcgccggg gaagtagagc
    15661 gtgttggtga gggccatcgc ctcgcgctcg agcagggcga gttcgctgaa gttgcgcacc
    15721 cggtggaggt cttggccaga gagcttcacg tcgaacacca ggtgataggg atggcggcgg
    15781 tagtcgtggt tgcccggagt catgaggatc ggcacgcgca actcctcgac ggtgggccag
    15841 tccggacccg gcgcccgacc caggatgagg tcgcgcagga agcccgcgtt gcccaagccc
    15901 tcgcggtcgt cgtccgtttc gtggacgtag tcgatgaggt cgccggtggc gacgatgaca
    15961 tccagctccc cggccgcgtg cagccggttg gcgaacgaca cgaagccacg gaactggtcg
    16021 ttcatgttgc acatgcgggc ggccgcttcg gtgagcccga ggtcgcgcag ggtgggtcgg
    16081 aaggagtcga tccgccgggc gacgtgggtg tcggtgatgt gcgcgagccc gaaatcggtc
    16141 cacgtcgagc ggtagtagac cgcgtgcggg gcgacgacct cccagccgac cgtcccagcg
    16201 cgcagcgcca cgtcatagag ctggcgctcg gccggtcggc ccagcagggg gaagccgacg
    16261 cctccgtcga ccggggtcga cggcccgagc tcgagcggga tgacagtggt ggggtcgccg
    16321 gaagccacga gcgcgatccg gtcggcgagg gccgcgcgca ccaattccgc actctccgaa
    16381 gggagtccga cgactgtgac ggtcaccctg tcggggccga ggaccatcac cgggtctgac
    16441 ccgttgccgg cttcggggaa gtgcggccgc cccagttgcg ggaaggcgaa gaaggcggcg
    16501 aggacgccgg tggataggcg cagctccagt cggtcgcggt gcaaggcggc ggcgacgcgt
    16561 atgccgcccg cgattcctgg ctgctggtag gtgatgtcga acgatgagac gggggcctgc
    16621 gtggtcatgg gatcggctcc ttcgaggcga tgtggtcaga aggtcgatgg gtcagaacgt
    16681 gggattgagt gagggggcgg gctgggccgc cggtcagagg gggagagcct cacccggtcc
    16741 gcggcttcgt ggacgggccg ggtgaggccg taagcacctt ggtccgtggg gtcagcggac
    16801 cgttcccgac atcgcctccc cagcggtgtt cgggtggtgc tcacctcggg catcgcccgc
    16861 attccgacgg gaagcgtcgg tgccggttcg ggcgagaccg aggggtcgtg ggagaccttc
    16921 aggtgcgggc gcaatacgtt cgtgcagtcg gaccagtggg gcgggagccc accagttcca
    16981 ccggcccatg aggcgcatga gggccggcac caggacgccc cgcacgaggg tcgcgtcgag
    17041 caggatggcc agggcgcagc cgagtccgag catctggatg aaggacacct tgctcgtcga
    17101 gaaggcgagg aaggtcaccg acatgagggc cgctgcggcg gtgacgatgc ggcccgtgct
    17161 gccgagcccg gaggcaactg cggccgtgtc gtccagtccg cggtcacgtg cctccttgat
    17221 ccggccgagg aggaagacct cgtagtccat cgacaggccg aaggccaggc agaacatcag
    17281 gggcgggatc gacaccgaca ggggcgtcgg ggtgaagccg agcaggcccg acccgtgccc
    17341 ctcctggaag acccacacca tcacgccgag caccgccgcg atggtggctg cgttggtcag
    17401 cagcgccttg acggggagca cgatggatcc ggtgaagaag aagatgacca ggaaggtcgt
    17461 cagggcgatg agggcgccga ccaggggcag tcggctcgcg atcgaggcat tgacgtcgac
    17521 gaaccgagcc gtcgggccac cgaccaacgg cgcggccggg gcgtctgcgc gacggatcgc
    17581 cgtgacgagg tcctgagcgg cttggctgta gacctccacg tcagggatga cgagaacggc
    17641 tgcggcagag tcggatagca agtccggtcg ggggccgcta actcgggcac cacgcacgta
    17701 cgtgccgacc ggagtgtcca ctcggctcac cgagggcagc acggacaggc tggcgccgta
    17761 tgccgcgagg gtcggacggt ccgtgcgttc cggggctccg tcggggaaga cgacggtgat
    17821 cgcctcgctc tcgcggctgg tgaagtcggc gcgcagcacc tcggccaccg cccggccctg
    17881 cgagtggctc accgggatgg accgatcgtc gggcagcccg aacgacacgc cggccagggg
    17941 cgcggcgagc acaccgagca ggaccaccac cggggccgcg gtgagcactg ggcgacgcat
    18001 gacgacggtg gcaacgcggc tccacaggcc ggcggcggca tacctggtgg gtttgccgaa
    18061 gcgccggtat gccgcgcggt gaccgagcag tcgcagtgcc gcgggcagga cgacgaggga
    18121 cacgccggcc gtgagcaggg tgacggcgat accggcatac gcgaaggacc ggaggaagta
    18181 catcgggaag gcgagcaacg ctccgagggc gaccgcgacc gtgatcgcgg agtagacgac
    18241 ggtccgtccg gcggtgtgca tcgtgactcg gagcgctccc tcgacgtcgt ctccggattg
    18301 gagttcctcg cggaaccggt tgacgacgag cagcgcgtag tcgatgccga gcccaagccc
    18361 gagagcggtg gtgaggttga gggagaagat gctgacgtcg gtgaccgcgg tgaggatgcg
    18421 caggacggcg agggtgccga ccatggcata ggcgcccacc agcatgggaa gcaatccggc
    18481 gatgaacgac ccgaacacca cgacgagcag cagcagggtg gcggggatgg cgagggcttc
    18541 ggcgagggcg aagtccttct tcacttgcag ggtgatgtcg gtgacggtct gggcggcgcc
    18601 gccggtctgg accgtgaccc cctcgggaga ggtcgtcgtg taggccggtt ggagggtggc
    18661 gatgcgttcg cggtagtcgt cctcgtcgcc gtcgatatgg gccactacga gggcagtgcg
    18721 cccaccggcg ccacgcagcg cggccgccgc acccgccggt gcgctccaga agtcggcgac
    18781 gacctcgacc cctggttcgg cgtcgaggcg actgacggct gctcggccga catccgcgac
    18841 gacggcgtcg tccaccgtcg cgcccgaggg ggccgtgacg agaaggagga agttggcggt
    18901 tggacggccg agtttctcgg ccagtgccct ggctgcctgg gcagactcgg ctgacggatc
    18961 gtcgaagccg cccgactgca gccggctgat ggcgccgacc ccgagcgccg cggcagcgac
    19021 gaggatgacc aggctggtga tgaggatcgc gcgagcccgg cgcatgacgg tggtgatgaa
    19081 ggtgtcgatc atggggagag tccttcgtgg gtttgatgcg gggaagctga tggaccggtc
    19141 aggcggccgg gtggtgtcgg gggatgtcgg gggcgagcgc ggctgctgcg atgcggacct
    19201 gttcgggatc gtccgggtgg ggggcggcgc caagggcccg ggcccaaccg cgcgccgccg
    19261 tgttggccga gtcgatgtgc gcgacaaggg tgtcgacccc tgcgtgtacg gccagccggg
    19321 cggcctccgc ggcgagcgcg gtgccgatcc ccagcccatg gcagcggtcg atgacctcga
    19381 ccgcgagctc ggcctcggtc ggccggagcg ggtcccgcag ccagcgtccg atgccgaccg
    19441 gtcgcccacc gaaggtggcc acgacggcga cgtggcgtcc ggggtcgaca tcggtgaggt
    19501 ggcgcagcat cgcgggtcgc aggaccgaca cgggggtgtg gtagcgcagc tggcgcgatt
    19561 tcgcgctgag cccctggaag acggcctgca gcggaccggc ctccaggggg gccaggggtc
    19621 ggatcgtcag gtagggatga gtggcggggg agaaggccgg aagggccggg gtgagccagg
    19681 gcatggggag agctccttgt gagtgctgac gttcgccgat ggtggggtga ggccctgccg
    19741 cgcccctgtc accggtctgt cacgtccccg gcggacccgg atgtcgggtg gcagactcgc
    19801 gggtatgccg tctctcaccc gcgccgaagc gacacgtcgc gctgcgcagt tggccgtgca
    19861 ggggtatgcc gtggcgctgg atctcgacgt cggcgacgaa cgtttcgcgt cgctgacgac
    19921 ggtgaccttc caggccctgg ccgacggggt gcagacctgg gtcgacgtca aggcgaccga
    19981 cgttcacgag gtgctgctgg acggcatacc ggtcgacctg gccgggtggc acgacgggcg
    20041 actggcgctg cccgccttgg ccgcgggtgc ccacacgctg agcgtcgacg cgacgatggc
    20101 ctaccggcgc gacgggtcgg ggctgcaccg ggcggtcgac ccggccgacg gggaggccta
    20161 tgtctatgtg cagtcgttcc tcgactatgc gccggcggtg ttcgcctgct tcgaccagcc
    20221 cgatctcaaa gcgtcgatca gcctgcgggt gcacgctccg acgcactgga ccatcgtcgg
    20281 caacggcgct gcgacgcggg ccggtgacgg tgagtgggag ctcgcggcca cggcgccgat
    20341 ctcgacctat ctgttcaccg tgtgtggcgg tcccttcgtc tccgtgcggg attcgcacga
    20401 cgggatcccg ctgggcctgc acgcgcgggc atcgatgcgt gccgagttgg agcgctgggc
    20461 gccacagatc ttcgaggtga ccaagcagtc gttcgacgcc tatcacgcgc tcttcgggat
    20521 tcggtatccc ttcggcgact atcaccaggt gttcgtcccg gacttcaacg cggtcgccat
    20581 ggagaacccg gggtgcgtga cgttgcgcga tgcgttgctc tatcgagggg cggccacgcc
    20641 cgacgaggtg ctgcgccgct cgcggaccgt ctgtcacgag atggcccaca tgtggttcgg
    20701 cgacttggtc accatgaagt ggtgggacga cctgtggctc aacgagtcgt tcgcggaata
    20761 cctcgcgcac cgggtgctca ccggcgcaac cgaattcgcc gacgcctggg tcgacttcgg
    20821 catccttcgc aaaccctggg ggtatgccgc ggagcgcgca ccgtccgccc accccgtcgc
    20881 gggggcgccg gcgctgacgg ccgaggacgc tctgtcggac ttcgacggga tctcgtatgc
    20941 caagggcgcc accgcgttgc ggcaactcat cgcgttcgtg ggagacgagg tcttcgttgg
    21001 tggtgtctcg aactacttgc gggacaaggc ttttggcaac ggaaccttcg ccgagtttct
    21061 ggcggcgatc gaagcgtccg cgggccgtga cctgggcgac tgggccaggg actggctgct
    21121 gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngacggcc cggtgctcac
    21241 gcgacgggcg cccgcggcat accccgcctc ccgcccgcac gtgctcgacg tcggtgtgta
    21301 tgccgcgagc gctccacccg aggtgacccg cgtcctggcc gaccgggagc gaacggcgtt
    21361 gccggacctg cgggttgccc cgggtgaccg gctggtcctg ccgaactgca gcgacctcac
    21421 ctgggcgtcg atcgacttcg acgaggccac gctggctgcc ctgccggccc ggctggggga
    21481 gttggccgac ccgctggccc gctcggtctg ctggcagtcc ctggatgacg gcgtggcccg
    21541 gggggtcgtt gacccacggc tgttcctcga tgcgatcgcg cggtcgtggc cgcgggagac
    21601 gcatcctgcg ctgttcgcca acgtcgccga cgacgccttg gtggtcttgt cgcgtttcgt
    21661 tcctgatgac ctgcgaccgg ggctgcgtgc ggagctggcc gagaccgcga ggaccgtcat
    21721 cgagagggag tcggacgggt cgccgcgggc gacgatcgcg gcccggctcg tggcccgggc
    21781 gtcgacccac gaggggctgc tgcggacctg gctggacgcc gggccgtcgt ggttgcaggg
    21841 cgacaccgac ttccggtggc tcgttgtcgg caacctcgcg ggcctggggc gcctgcgcga
    21901 ggaggagttg gcggccgccg aagccgccga cccgacggtg tccgggcggc tggccgggtt
    21961 gttggcgcgg gcctcggtgc cgacggtggc cgctaagacc tgggcgttcg agcaactcgt
    22021 cgatcccgcg tcgggtcaca ccaaccatgc cctcgtcgag ttggcgcgtg gcctgtggcg
    22081 ctcgcccgac cgagggctcg ttcgaccgtt cgtcgagccg ttcctcgacg ccattccgcg
    22141 gatgaccgca tgggtggggg acgacgcgct gacgaaggtg gtgcgcttcg gcttcccgtt
    22201 cgtcgtcgag gcgcgcacca tcgagctcgt cgacgccgca ttggcccgca acgacctgac
    22261 ccccggcgtg cgtcgggcct tcgtcgagtg gtcgtggccg gtgcgggagg ccctggcgtc
    22321 gcgcagccgc tggttcccca ccggctgacg cgagggcggc gctccgccaa tcggggtgtg
    22381 cggcataccc actggtaact gcccatggca gggcctggag agctagcctc ggatggagcc
    22441 acccgccggc gacgacgcga ggaggtgaaa gcgcggtggt gacacctgcg cagcacgcag
    22501 ccgccgtgcg cgcactctcc ctcagttacg agcacctcga cccggacgtc cccgtgcggc
    22561 tggccaagcg gacgaccaac ctgttccgcc cacgggcggc tgcgatgggt ccgggcctgg
    22621 atgtgtcggc gctgaccggg gtgatcgacg tcgacccggt ggccgggacc gcgacggtgc
    22681 aggggatggc gacctacgag agcgtcgtcg acgccaccct cgcggccggg gtcatgccgc
    22741 tcgtcgtgcc gcagttgcgc accatcaccg tgggcggtgc cgtgacgggc ctggggatcg
    22801 aggcgagcag tttccggaac gggctgccgc acgaatcggt ccgctcgatg gacatcctca
    22861 tcggcacggg cgagatcgtc accgcggcgc cgacgggccc gcacgctgac ctctttcgca
    22921 ccttccccaa ctcctacggc tccctcgggt atgccgtgtc tctcaccatc gacgtcgagc
    22981 cggtggcgcc gttcgtccgg ctgcgacacc tgccgttcga ctcgttgcag tcgctgaccg
    23041 aggcggtcgg gcagatcgtc gcggcgcggg cctgggacgg cgagccggtc gacttcctgg
    23101 acggggtcgt cttcgcgagg aacgcggcat acctcacgtt ggggcgtttc gcgcagtctc
    23161 tcgatgcggc cgaaacgccg agcgactatg cgggacaacg gatcttctac cgctcgatcc
    23221 gcgagcggag caccgatgtg ctcacggtgc gggacttcct gtggcgctgg gacaccgact
    23281 ggttctggtg ctcgcgggcg ttcggggtgc ag
//
LOCUS       DAORPW010000024        22849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_368_length_22849_cov_720.127226, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000024 DAORPW010000000
VERSION     DAORPW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 22849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22849
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_368_length_22849_cov_720.127226"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..204
                     /locus_tag="PLB86_03630"
     CDS             31..204
                     /locus_tag="PLB86_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HRC64256.1"
                     /translation="MAAMKPRTGDGPLEVTKEGRGIVLRMPLEGGGRLVVEMTPDEAA
                     ALRDAIAGCDGVS"
     gene            complement(276..908)
                     /locus_tag="PLB86_03635"
     CDS             complement(276..908)
                     /locus_tag="PLB86_03635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRC64257.1"
                     /translation="MSANRAASWAYAEQFIAEGPTMEAARERGQALGAVPVGVATGAA
                     LRMLAATGAAKAVVEIGTGAGVSGLWLLAGMPSDGVLTTIDVDADHQRAAKEAFAEAG
                     IAHQRTRVILGRALDVLARMTDAAYDLAFVDGDKDEYPAYVREATRLLRPGGVLALDN
                     MFWHDQVADPTARDERTRTLRDLGKALREDDAFASALLPVGDGLLVAVKR"
     gene            1130..1804
                     /gene="sigE"
                     /locus_tag="PLB86_03640"
     CDS             1130..1804
                     /gene="sigE"
                     /locus_tag="PLB86_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigE"
                     /protein_id="HRC64258.1"
                     /translation="MIRPLTGSDTVPDEGSGWTPPTWEEIVTQHSARVYRLAYRLTGN
                     PHDAEDLTHDVFVRVFRSLNSYTPGTFEGWLHRITTNVFLDKMRRKQRIRFDALPDDA
                     ASRMASREPGPEQVFTDTHFDDDVQRALDALAPEFRAAVVLCDIEGLSYEEIAATLGI
                     KLGTVRSRIHRGRAALRESLAHRAPGADTAAARVTTPAPAASRPAARHGIPLPSRLRP
                     AARAGA"
     gene            1804..2409
                     /locus_tag="PLB86_03645"
     CDS             1804..2409
                     /locus_tag="PLB86_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64259.1"
                     /translation="MSRHLGDQVSAYVDRRLPGSALSAWDRHVVVCAHCRYAVDQERS
                     LLASLRTAPAPCVSDSLQALLLGMGNASPNARFIAAAASTGSPVGAQPPVPAAPFLVH
                     GLRLPTVSPAGPPRHRSARRAALVAGLAAGASAAAAWTVGLAPASVSAAPAGAPVPAR
                     VGTASGLGAAYSGMLSFPVSQVGFSTVWPTSTTSTTEISGR"
     gene            2406..3761
                     /locus_tag="PLB86_03650"
     CDS             2406..3761
                     /locus_tag="PLB86_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRC64260.1"
                     /translation="MSEHPDRPAFDPGAGSAPASWPDPASDPRSGVPTAALPAYPPAA
                     AAPPPSGYPPPSVYPPPPSAGVFPPAYAVPAPPAPASAAAAVPAAAARQPGSRVGLMV
                     ALAAVVGLLAGLLGGGVGSVLVNRGVAVVQGQSAVPAPLPGVTARPDGSIAKIAATAL
                     PSVVTVKIRTASASGNGSGFVIDRAGHILTNNHVVSGATGPGEITVEFVDGASVAARI
                     VGRDSSYDLAVLAVDKTDVTPLEWGRSSDVVVGDGVIAVGAPLGLEATVTSGIVSALN
                     RPVVATGGDDMSFINAIQTDAAINPGNSGGPLLDMSGRVIGINSAIARDPGSTSSSGG
                     SIGVGFAIPSDQAAKTAQQLITTGKAEHPIIGVKLDTQFTGEGARIADPGGVTPGSAA
                     DTAGLRDGDVIVRFEGKKVPGSNQLVVAIRAKSVGDTVHLTVERAGAQMDITLTLRAS
                     N"
     gene            3807..4169
                     /locus_tag="PLB86_03655"
     CDS             3807..4169
                     /locus_tag="PLB86_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sec-independent translocase"
                     /protein_id="HRC64261.1"
                     /translation="MFDINGWELIVLAMVAVVVLGPERLPVYAAKLARAVRQVRTLAE
                     GARSQLKEQLGPDFDDVNWRQYDPRQYDPRRIVREALMDPAEPAAQAEARVWAPEEQP
                     GRAAYDPGKPTPWDSEAT"
     gene            complement(4211..5353)
                     /locus_tag="PLB86_03660"
     CDS             complement(4211..5353)
                     /locus_tag="PLB86_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRC64262.1"
                     /translation="MSLVALPTQDALLAALAKVNDPEIRRPVTELGMVKSVSVDDAGH
                     VALELFLTVAGCPMKDTLRKDTSAALLRVPGVVSVDVALDVMSDEQRAALRTQLRGGV
                     AEREIPFAKSGSLTRVYAVASGKGGVGKSSVTVNLAAALAAEGLKVGVVDADVHGFSI
                     PRMLGVTGLPTQVDDMILPPISHEVKVISIGMFVPGNQPVVWRGPMLHRALQQFLGDV
                     FWGDLDILLLDLPPGTGDIAISIGQLLPTAEILVVTTPQQAAAEVAERAGSIALQTHQ
                     RVVGVVENMSWLELPDGSRQEIFGSGGGKTVADSLTRLIGAPVPLLGQVPLDAGLREG
                     GDSGTPIVLSDPDSPAAVVLRAVAKGLAQRSRGLSGRSLGVSPVKR"
     assembly_gap    5461..5560
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5561..6039)
                     /locus_tag="PLB86_03665"
     CDS             complement(<5561..6039)
                     /locus_tag="PLB86_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HRC64263.1"
                     /translation="MADRRRPNRTPGRGRGARLDQPREFRRRDFLPGPGFSQESFGVW
                     SEQFARFMGTPLFLIGLTAFAVIWIGWNTWAPVAWQFDPRELNYTLLTLLLSLQASYA
                     APLILLAQNRQADRDRVTVEQDRARDERNLADTEYLTREVAALRLALREVATRDFVRS
                     "
     gene            complement(6032..>7001)
                     /locus_tag="PLB86_03670"
     CDS             complement(6032..>7001)
                     /locus_tag="PLB86_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRC64264.1"
                     /translation="STNTRDADARPSIDATVEDLAVDIRPKAWTVTKVYVRKLVHAPR
                     RGLHLNRRRGETLLVDIADVSGLDTDPRESDTAALLAAYRDGAVADLADAIHDLARDR
                     RRQVATALDDDRLADVLEQLPEDDQVEILAGLDDERAADVLEAMEPDDAADLLAELPA
                     AHREGLLARMEPDEAEPVRRLMAYDEHSAGGLMTSEPVILPPEATIAEALALVRREEV
                     STTLATTVFVCRPPLETPTGRYLGIVHFQKLLREPPHAAVGSVLDKVKPLHPASTVDH
                     VHRMFATYDLMAAPVVDGHGRLVGAVTVDDVLDHILPEDWREGTHG"
     assembly_gap    7002..7101
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7102..7517)
                     /locus_tag="PLB86_03675"
     CDS             complement(<7102..7517)
                     /locus_tag="PLB86_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HRC64265.1"
                     /translation="MSLPKRVFLAQLAGLPVFDPLGDQVGRVNDIVVAMPTGYAATPT
                     GYAATPTTPRVIGLVVEVPGRKRVFVPTTRVTSLEESQVITTGVVNLRRFEPRAGEVL
                     AIAGLRERRVIVRLAPGSRTAEATAAQSTNTQSTNTR"
     gene            7632..8717
                     /locus_tag="PLB86_03680"
     CDS             7632..8717
                     /locus_tag="PLB86_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRC64266.1"
                     /translation="MRHPKDFYAPLAVGAPMPMRAVPARPSRVIHFFDPSNAKMAAKI
                     PEMVGTVDVLLGNLEDAVKADNKEAARAGLVAIGKSTDFGDLTQFWTRINSLDSPWAL
                     DDLTTLVSEIGDKLDVIMVPKVQGPEDIHYVDRLLAQLEAKAGLTRPILVHAILETAR
                     GMANVEEICFASPRMQGLSLGPADLAADRRMKTTRVGGGHPGYLVREDPPGGDINGPR
                     AIFQQDLWHYTIARMVDACAMAGIFPYYGPFGDIKDVVACEDQFRNAFLLGCVGAWSL
                     HPAQIAIGKKVFSPSAADIAHARRVVAAMGDGTGAVMLDGKMEDDASLKQCLVMIELA
                     ERLAKTDPELASLYAAAPDPAAATSAG"
     gene            8808..9698
                     /locus_tag="PLB86_03685"
     CDS             8808..9698
                     /locus_tag="PLB86_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRC64267.1"
                     /translation="MSPAKKHGTRYRPRRSVLYMPSSNERALEKAKSLPCDALILDLE
                     DAVAPDAKEAAREAACAAVKSGEYGRKELIIRVNALGTDWHYPDMMAACKAGPAGILV
                     PKVNSAKEVHDLVREMERYKTPKHTKLWAMIETPRSVLDVGRIAAASDRLAVLVMGTN
                     DLVKELDAQHLPGRVPIVPALALAVLSAKASGLFIIDGVYNDVRDPEGFAVECAQGRS
                     MGFDGKTLIHPDQVGPCNEAFSPSDEQVADARGLIDAFDAAVAEQKAVATYKGKLVEH
                     LHVTMAEKVLATREAIDLLG"
     gene            complement(9732..10490)
                     /locus_tag="PLB86_03690"
     CDS             complement(9732..10490)
                     /locus_tag="PLB86_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC64268.1"
                     /translation="MSAEVARVDETNEVDHVDQLDPESPDVPDSADAAAPIRVLLVDD
                     QELFRAGVAVVVGADPGINVVGEASDGREAVALVDALEPDVVLMDIRMPELDGVEATR
                     QIFAPERAARRPRPVRVIVLTTFNLDDRAATAIRYGASGFLLKDATPDVLREAIRTVH
                     LGNAVLSPADLGSLLEGDFRRRSATPAAYSQLTEREREVFAGVARGLSNAEIASTVFV
                     SESTVKTHVGAILRKLDLRDRVQIVVFAYEHALV"
     gene            complement(10507..11745)
                     /locus_tag="PLB86_03695"
     CDS             complement(10507..11745)
                     /locus_tag="PLB86_03695"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC64269.1"
                     /translation="MTRHVRRWWPDAALGVLVASAGAFELSARSIGRWWMVAVLAVAV
                     GLFRRAPVAAVALLWVLLYAHLRTGSTLLITEVTTALVAYGAARWGRTVTVWFSGLSI
                     LALGVLGVWLVGTRLDIVLPLVPLVDAATLKRVLVDPPGVGTQVALIPLGLALVGFGP
                     PWLLGLAVRSIDEARRSRALQHLAEDHAALAHQQVEREREIAELRAGQARLARDVHDV
                     VGHSLAVILAQAQAAAYLQDPDEVRATVDRIADAARRSLQDVRTVLESTQVEGAAVRA
                     QRPPGTLDALVTGLRDAGHGVESTVIGIPQPLPPELETVAYRVAQEMLTNAVRHGDRR
                     EPVHVEQVWSTHSGDGAGQLRLEVRNTVAADAAPPQRPGLGVDGMVRRLASVGGSLDA
                     RLHDGWFVATASLPVRAVLR"
     gene            complement(11790..12755)
                     /locus_tag="PLB86_03700"
     CDS             complement(11790..12755)
                     /locus_tag="PLB86_03700"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRC64270.1"
                     /translation="MTRVAVIGGGRSSEHEVSLASAAAVADGLEQAAYDVLRLTITRD
                     GAWLADTPLPLAAAVAAVQECDVVFPAVHGPHGEDGTLAALCEVAMVPYVGCGVGAGA
                     IGMHKWATQLVAESVGVATARGRLLTDDGPIPWTGPCVVKPLRAGSSHGVTLVERPGD
                     LGAAVKAAFHCDTTVLVEQFVVGREIDLGVIEQPDGTWTVLPPLEIGRSGIFDTTTKY
                     DGSADFRVPAALADADRHALDDAALQVVDALGCRGLARVDFFLTEAGPILNEVNTMPG
                     LTPHSQLPRMASAAGLDYPDLLDLLVTTAVASRQRGRVPAVAAQC"
     gene            complement(12752..13900)
                     /gene="alr"
                     /locus_tag="PLB86_03705"
     CDS             complement(12752..13900)
                     /gene="alr"
                     /locus_tag="PLB86_03705"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRC64271.1"
                     /translation="MSAAVSVSLRPAAPTAGAVLDIDLSAVGANTRRFAALTPALLAV
                     VKADGYGLGAAQIARTALSHGAIGLGVTSLAEALALRADGLTAPILSWLNPVDGDFAA
                     GLAAGVHLAVGSIEHLDAVCRAARRTGRVASVHLAVDCGMARDGSPPLAWPALCARAG
                     QAQQSGLVDVVGVMGQLSHAGRPRHAASQRGRAVFDWAVTTAAGHGLAPEHRHLATTA
                     ACLTDPRSMHTMSRVGAGLVGVDPSSTTLLAQVVTLRAPVVAVRDVVAGTGVGYGHTW
                     RAKRATRLVTVPVGYADGIPRGAAGRAEVSVGGHRCPVVGGVNMDQTVIDVGRLEIEL
                     GEPVVVLGGEGPTLVEWAEWAQTLPHEILTGLGGRVARRYSGVSRDQR"
     assembly_gap    14005..14104
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14136..14939)
                     /locus_tag="PLB86_03710"
     CDS             complement(14136..14939)
                     /locus_tag="PLB86_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRC64272.1"
                     /translation="MELTRRGALRVGVRAVGAIVLAGCTAAPTPSPGTLGSEGALTST
                     HWPGASPRWRLVRPDPAVVPSPRLVVALHGKGGSVGDASFLGLFDQVARLGVAVAAVD
                     CGGTYFHARRDGSDVGAMVIDDLLPVLRTQGVSPAPVGLLGWSMGGYGALWLAARYGR
                     DVIGAVGCMSSALWTSPGASAPGAFDDRADFVAHDVFARTKAFAGMPLRLDCGTSDPF
                     IAANRAFAKLVPDAVVTFDAGGHDDTYWRAHGIRQLEWFAQNLPAVSPT"
     gene            15272..15736
                     /locus_tag="PLB86_03715"
     CDS             15272..15736
                     /locus_tag="PLB86_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HRC64273.1"
                     /translation="MRLLRFLPAVVELALLVYCLVDCIQTERSRVRTLPKLGWILLII
                     ILPIIGGVGWLLAGRPRRDGTGDHGAFGTAIRGLGGGPDSSAPRRPLAPEDDPNFMRG
                     LDHGNPEKERLLEEWEAERARREAQEPVAPKDAEDAPGETPGSQERPTDAPG"
     gene            complement(15741..16580)
                     /locus_tag="PLB86_03720"
     CDS             complement(15741..16580)
                     /locus_tag="PLB86_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HRC64274.1"
                     /translation="MSHSPSSDEPAEFAVDSHPDAPPDGDRWSTWDGAAHGPGPRPSW
                     VVTDLGAVDADLGVLKTGKEADVHVVRRWVPGDPTHDVFLAAKRFRNSKHRMFHRDAG
                     YLEGRRVRRSRENRAMARRTDFGKEMISGRWAFAEFETLGALWTAGLPVPYPVQFDGT
                     EILMEFIGADGLAAPRLADSRPPETALAGLFDQVREAMLSLAEMGWAHGDLSPYNVLL
                     DVDRIVLIDWPQIVDVIGNPHGFEFLERDARTMAAWFTRRGYAVDPDEFFAQLLGAAT
                     SRW"
     gene            16794..20108
                     /locus_tag="PLB86_03725"
     CDS             16794..20108
                     /locus_tag="PLB86_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRC64275.1"
                     /translation="MPDKSLGRGGLRVSYPSTHTVGVPVPRKDRDLGAVDTHAILLEA
                     LRDNDFEKVDEFTLTPEVRRDRDPLPRDPGKLTMELDVEGEQDAVVLVEKDGCYSWHL
                     PTGDKARRRGLPGEKRAVRFDIDLAVSDAAYANRRVERGLDAGPRTRGLFGDLLAGAV
                     RVVVMKFAAPLLVGAAVGFLERHVEPGIVHITAPDPAIWQRVERLDQLGLPTDRPVRI
                     LLFIHGTFSSTLSAFGSMAVSDNGARFLESALRQYDAVIGFDHPTLSVDPMVNATDLL
                     TRVSSVTNEVSFDVITHSRGGLTTRSFAEYVLPGSGWNGRVEKAVFVAATNNGTHLAD
                     TDRWYDLIDIATNLTMVGAGVLAAMPGGAPVAAVVAGVVKGLGALVKYLGAYAVEEDG
                     VPGLAAMRPGGPFVTGINQTQPGQPTPGTPWFVAKSNFHVTVGDDSHRPPEFPKELAV
                     RLAEGLVDQVFRGDNDLVVDCESMGSIDAAVGGGFVADTYDFESNDVVYHGNYFLQPD
                     FVRRMTDWLLWRVEAAMAEPPEAAAEPPEEAAEPPEVVRGIDFGEPPPPPAAPPPPVR
                     GVDVPADPGAPPAAPNVAEATLLASMTAKPSVGVPTALRVALSRKELHVAAGEVGAVG
                     GFVAPADRPLAVNVAASANARVGLAPDQLGEFATDSFQLPPGGGTSELEFFVEAQAAG
                     PVTVTVVVQDGVKWLKSLALTATAGAPVAPVAAAVFAAPGPLQPEVLASASTPLPASA
                     DQPDFEDYFTIQVQEARFGADTKFLYVVSCPRLGISERFESLPLRARSDYIAATLADV
                     KRAWDDHGAGAAYLETLQDIGADLFGTLFPEDLRALLWANRSKLAPMVLRADEPDVPW
                     ELIHLKPANGTRQKAPAFLGQLGMLRWPFDGYPQATLRARPGRVRVLCPDRAGPAAPG
                     AGVRQEADFLEQRLGASSLPGTEAALRTLLRGGDFDLLHFSGHGVADPKDIGSAKLLL
                     TQVLEGGGAVKLRYLTARAVEQNARLRRDDGSGPLVVLNACQLGRGGDQLTGSGGFAK
                     AFLSTGAAAYVAALWSIHDTPAHDFVTSFYDRLLAGDTVAESAAAAREKARAAGDATW
                     LSYVIYARPDARLVTQ"
     gene            20195..21037
                     /locus_tag="PLB86_03730"
     CDS             20195..21037
                     /locus_tag="PLB86_03730"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRC64276.1"
                     /translation="MTNRALCVGINQFSNLPMASWLNGCVNDANDMAAMLRKRGFAPR
                     SVTVLTDDQATKANVMAALTKLVETAKEGDHIVFSFSSHGTQVPDTDGDDGRPETDGA
                     DEAFACYDMAQKGDQWDPDTVIIDDELRVLFGKLPKGVVMEAYLDTCHSGDGLRAEDL
                     LAGRRPRFVPPPTLKGLRVIEAAATKSTTTELVKAIPAGGRPVLYAGCRSDQTSADAT
                     FDGRSSGAFTYFFLKALAANPAGTRAEIHKALTKGLVDASFSQRPTLEGPPKAKKAGV
                     GQAW"
     gene            21126..21500
                     /locus_tag="PLB86_03735"
     CDS             21126..21500
                     /locus_tag="PLB86_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRC64277.1"
                     /translation="MSHGKDVLDALRDETAALREKIPGVYEGFAHTHKAALGDGALST
                     KAKELIAFGIAVSKQCDGCIASHARGAARAGATLEEAAEAIGVAILMNGGPATVYGPR
                     AYAAFSEFHAQFARLRGAQTPG"
     gene            21646..22848
                     /locus_tag="PLB86_03740"
     CDS             21646..22848
                     /locus_tag="PLB86_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC64278.1"
                     /translation="MSRLQTTDGLTDDQKELLKLVKEFVDEQIIPVATELEHRDEYPQ
                     AIVDGMKEMGIFGLMIPEEYGGLGESLLTYALCVEEIARGWMSVSGIINTHFIVAYML
                     LQHGTEEQKQRYLPRMATGEVRGAFSMSEPALGSDVSAIKTKAVKDEQGDGWTINGQK
                     MWLTNGGSSNLVAVLVRTETGADSVYKNMTTFLVEKESGFGETAPGLTVPGKIEKMGY
                     KGVDTTELVFEDYRTNDAQILGGVPGKGFYQMMDGVEVGRVNVAARACGLSMRAFELG
                     IRYSQQRETFGKKIAEHQGILFRLADMAVKVEASHQMMVKAARLKDAGKRNDLEAGMA
                     KYLAAENCADVVEQSFRIHGGYGYSKEYEIERLYREAPMLLIGEGTAEIQKMIIGRRL
                     LEDYALKR"
BASE COUNT         3529 a         7893 c         7857 g         3270 t
ORIGIN      
        1 ggcgcacgag tttccgagga aggggaaccc atggcggcga tgaagccgag gaccggagat
       61 ggtccactcg aggtcaccaa agaaggccgc gggatcgttc tgcgcatgcc tttggagggt
      121 ggcggacggc tggtcgtcga aatgaccccc gacgaggctg ctgcattgcg cgacgcaatt
      181 gcaggctgcg acggcgtgtc ctgacgtacc acccacgagc gaggcctccg ttccgaccag
      241 gaacgggggc ccgttggtgt tttcgggcct cgccgtcagc gcttgacggc gaccagcagc
      301 ccgtctccga ccggcagcaa cgccgaggcg aacgcgtcgt cctcccgcag ggccttcccc
      361 aggtcgcgca gcgtccgggt gcgctcgtca cgggccgtcg ggtcagccac ctggtcgtgc
      421 cagaacatgt tgtccagggc cagcacccca ccagggcgca gcaagcgggt cgcctcgcgc
      481 acgtaggccg ggtactcgtc cttgtcgccg tccacgaacg ccaggtcgta tgccgcgtcg
      541 gtcatccggg cgagcacgtc cagcgcccgc cccaggatca cccgggtgcg ttggtgagcg
      601 atccccgcct cagcgaacgc ctctttggcc gctcgttggt gatcggcgtc gacgtcgatc
      661 gtcgtgagga ccccgtccga cggcataccg gccagcagcc acagccccga gacgccggcc
      721 cccgtcccga tctcgacgac ggccttggcg gcccccgtgg ccgcgagcat ccgcagcgcg
      781 gcgccggtgg cgacgccgac gggcacggcg cccagagcct ggccccgctc gcgggcagcc
      841 tccatcgtgg ggccctcagc gatgaactgc tcggcatacg cccaactggc ggcacgattg
      901 gcgctcatgg tcgaaaccct agcccgcgag agtcctcgac gcagggagca cgcggacctt
      961 gcggcgtcga gacctcaggg gcggatgggt ggaataccag ggtcgacccg gatggttggc
     1021 caatacacag gcagggcaca gtcgactcgt gggcatcgag ggaactcttg aggtgtcacg
     1081 aacgtcagaa gtccaaggct gcgaaaggcg cgaggtagtg agcgacaccc tgatccgtcc
     1141 cctgaccggg agcgacaccg tccccgacga gggttccggt tggacgcctc cgacgtggga
     1201 ggagatcgtc acgcagcact ctgcccgcgt ctaccggctt gcctaccgcc tcaccggcaa
     1261 cccccatgac gccgaagacc tcacccacga cgtgttcgtc cgggtcttcc gatcgctcaa
     1321 ctcctacacg cccggcacct tcgagggctg gctgcaccgg atcacgacca acgtgttcct
     1381 cgacaagatg cgccgcaagc agcggatccg cttcgacgcg ctgcccgacg acgccgccag
     1441 ccgcatggcc agccgcgagc cggggcccga gcaggtcttc accgacaccc atttcgacga
     1501 cgacgtccaa cgcgcgctcg acgccttggc gcccgagttc cgtgcggccg tcgtcctgtg
     1561 tgacatcgag ggcctgtcct acgaggagat cgccgccacc ctggggatca agctcggcac
     1621 cgtccgctcc cgcatccacc gcggtcgcgc cgcgttgcgt gagtccctgg cgcaccgggc
     1681 gcccggcgcc gacaccgcgg cagcgcgagt gaccaccccc gctcccgccg cgtcccgacc
     1741 ggccgcgcgt cacggcatac cccttcccag ccggctgcgc ccggccgccc gcgcaggagc
     1801 gtgatgtccc gacacctcgg cgaccaagtc agcgcctacg tcgatcgccg gctcccgggg
     1861 tcggcgttgt cggcgtggga tcggcacgtg gtcgtctgcg cacactgccg gtatgccgtc
     1921 gaccaggagc gcagcctgct cgcctccctg cgcaccgcgc cggcgccgtg cgtgtccgac
     1981 agcctgcagg cgttgctgct gggcatgggc aacgcgtccc cgaacgcccg cttcatcgcg
     2041 gctgcggcct cgacggggtc tcccgtcgga gcccagcccc cggtcccggc cgcgccgttc
     2101 ctcgtccatg ggttgcgtct gccgacggtg tccccggccg gcccgccccg gcatcgctcg
     2161 gcccgccggg ctgccctcgt ggctgggctg gctgccggag cctcggccgc tgctgcctgg
     2221 accgtcgggc tagctcccgc atcggtcagc gctgctccgg ccggtgcgcc ggtcccggcg
     2281 cgagtgggca ccgcgagtgg tctcggcgcg gcatactcag ggatgctgtc cttccccgtg
     2341 agccaggtgg gcttctccac cgtgtggccc acctccacga cgagcacgac ggagatctcc
     2401 ggcagatgag cgagcacccc gaccgtccgg cgttcgaccc gggcgcgggt tctgcccctg
     2461 cctcctggcc cgacccggcg tccgacccgc gcagcggcgt cccgaccgca gccctgccgg
     2521 cctatccgcc ggccgcagcc gcaccgccgc cttccgggta tccgccacct tccgtgtatc
     2581 cgccgcctcc cagcgctggc gtcttccctc ccgcgtatgc cgtccccgcg ccgcctgcac
     2641 cggcctccgc agccgcggcg gtccctgcgg ccgcggcgcg ccagccgggc tcccgggtcg
     2701 gcctcatggt ggcgctcgcc gccgtcgtgg gcttgctggc cggactgctc ggcgggggcg
     2761 tcggcagcgt gctggtcaac cgcggtgtgg ccgtcgtcca agggcagtcg gccgtgcccg
     2821 ctccgctgcc tggagtgacg gcgcggcccg acggcagcat cgccaagatc gccgcgacgg
     2881 ccctgccctc ggtggtcacc gtcaagatcc gcacggcgag cgcgtccggc aacgggtcag
     2941 gcttcgtcat cgaccgggcc ggacacatcc tcaccaacaa ccacgtcgtc tcgggggcca
     3001 ccggtcccgg agagatcacc gtcgagttcg tcgacggcgc gtcggtcgcg gcgcggatcg
     3061 ttgggcgtga ctcgtcctac gacctggccg tcctggcggt cgacaagacc gacgtcacgc
     3121 ccctggaatg gggccgctcg tccgatgttg tcgtgggcga cggggtcatc gcggtgggcg
     3181 cgccgttggg tctggaagcg accgtgacca gcgggatcgt ctcggccctc aaccgtcccg
     3241 tggtggccac gggcggcgac gacatgagct tcatcaacgc gatccagacg gatgccgcaa
     3301 tcaaccccgg caactccggc gggccgctgc tggacatgtc cggccgggtc atcgggatca
     3361 actcagcgat cgcccgcgac cccggctcga catcgagcag cggcggcagc atcggcgtcg
     3421 gattcgcgat ccccagcgac caggccgcca agaccgccca acaactcatc accaccggta
     3481 aggccgagca cccgatcatc ggggtcaagc tcgacactca gttcacgggt gagggtgccc
     3541 gcatcgccga cccaggggga gtgaccccgg gcagcgcagc cgacacggcc ggtctgcggg
     3601 acggagacgt gatcgtccgc ttcgaaggca agaaggtccc cgggtcgaac cagttggtcg
     3661 tggccatccg ggccaagagc gtcggcgata cagtgcatct cacggtcgag cgggccgggg
     3721 cccagatgga catcaccctg acgttgcggg catccaacta ggcgcacaac gacccacggc
     3781 gggcggcgga agggaggcgc gggcagatgt tcgacatcaa cggctgggaa ctcatcgtcc
     3841 tggcgatggt cgccgtcgtc gtcctcggcc ccgaacgcct cccggtgtat gccgccaagc
     3901 tcgcccgcgc ggtgcgccag gtgcgcacgc tggccgaagg ggcccgcagc caactcaagg
     3961 aacagctcgg ccccgacttc gacgacgtca actggcggca gtacgaccct cggcagtacg
     4021 accctcggcg catcgtccgc gaggcgctca tggacccggc tgagccggcc gcccaggcgg
     4081 aggcccgcgt ctgggcaccg gaggagcagc ccgggagggc ggcatacgac ccggggaagc
     4141 caaccccctg ggactctgag gcgacctgac cggtcggccc ccgcgaggag gccgccgggg
     4201 cacggctgag tcagcgcttg accgggctga cgccgagtga gcgacctgac aacccgcggg
     4261 agcgctgggc cagccccttg gcgaccgcgc gcaggacgac cgcggcgggg gagtcggggt
     4321 cactgagcac gatcggggta cccgagtcgc cgccttcgcg cagaccggcg tcgagcggca
     4381 cctggccgag caacggcacc ggagcgccga tgaggcgggt gagcgagtcg gcgacggtct
     4441 tgccgccgcc cgatccgaag atctcctgac gcgatccgtc gggaagctcc aaccacgaca
     4501 tgttctcgac gaccccgacg acccgctggt gggtttgcag ggcgatcgag ccagcgcgct
     4561 cggcgacctc cgccgcagcc tgctgcgggg tggtcacgac gaggatctcg gcggtgggga
     4621 gcaactgccc gatggagatg gcgatgtcac ccgtgcccgg cggcaggtcg agcaggagga
     4681 tgtccaggtc accccagaag acgtcgccga ggaactgctg cagcgcccgg tgcaacatcg
     4741 ggccacgcca gaccaccggc tggttgccgg ggacgaacat gccgatggag atgaccttca
     4801 cctcgtgcga gatgggcggc aggatcatgt cgtcgacctg ggtggggagc ccggtgacac
     4861 cgagcatccg cgggatcgag aagccgtgga cgtcggcgtc gaccacgccg accttgagcc
     4921 cctcggcggc cagggctgcg gcgaggttga cggtgaccga cgacttgccg acgccgccct
     4981 tgccggaggc cacggcatac acccgggtga gggagccgct cttggcgaag ggaatctcgc
     5041 gttcggcgac cccgccgcgc agttgggtgc gcagggccgc gcgctgctcg tcgctcatga
     5101 cgtcgagggc gacgtcgacg gacaccacgc ccggcacgcg caggagggcg gcgctcgtgt
     5161 ccttgcgcag ggtgtccttc atggggcagc cggcgacggt caggaagagc tcgagggcca
     5221 catgcccggc gtcgtcgacc gacaccgact tgaccatgcc caactcggtg accggtcggc
     5281 ggatctcggg atcgttgacc ttggcgaggg cagcgaggag ggcatcctga gtggggagag
     5341 cgacgagcga catgggctcc atcgtaggtg gctgatcggg tcagtcggac cgacgttcac
     5401 cagtgcccga cggcagccgc gggctgctgg acgacggctc ggtggttgcc cgctcgtcca
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaacgcacga aatcgcgggt
     5581 cgcgacctcg cgcagggcca atcggagcgc agccacctcg cgggtgaggt actcggtgtc
     5641 ggcgaggtta cgttcgtcac gggcccggtc ctgctcgacg gtcacccggt cacggtcggc
     5701 ctgacggttc tgggcgagca ggatgagcgg cgcggcatac gacgcctgca gcgacaggag
     5761 caaggtgagc agcgtgtagt tgagctcccg cgggtcgaac tgccacgcca ccggcgccca
     5821 ggtgttccag ccgatccaga tgacggcgaa ggccgtgaga ccgatgagga acagcggcgt
     5881 ccccatgaac cgggcgaact gctcgctcca cacgccgaag gactcctgcg agaacccggg
     5941 tcccggcagg aagtcgcgcc ggcggaactc ccgaggttgg tccagccggg cgccccgacc
     6001 gcgtcccggg gtccggttgg ggcgccgtcg gtcagccatg ggtgccctcc cgccagtcct
     6061 cggggaggat gtggtccagg acgtcatcga cggtcacggc gccgacaagg cggccatggc
     6121 cgtcgaccac cggcgctgcc atgaggtcgt aggtcgcgaa catccgatgc acgtggtcga
     6181 cggtgctggc tggatgcagt ggcttgacct tgtccaggac cgagccgacg gcggcatgcg
     6241 gcggctcacg cagcaacttc tggaagtgga cgatccccag gtaacgaccg gtaggcgtct
     6301 ccaacggtgg gcggcagacg aagaccgtcg tcgcgagcgt cgtcgagacc tcttcgcgtc
     6361 ggacgagggc cagcgcctcg gcgatcgtcg cctcgggcgg gaggatgacc ggctcgctgg
     6421 tcatcaagcc gcccgcgctg tgctcgtcgt aggccatcag ccgtcggacc ggctccgcct
     6481 cgtcgggctc catcctggcc agcagcccct cgcggtgggc cgcgggcagc tcggcgagca
     6541 ggtcggcggc atcgtcgggc tccatcgcct ccaagacgtc ggcggcccgt tcgtcgtcca
     6601 ggccggcgag gatctccacc tggtcgtcct cggggagttg ctcgaggacg tcggccaggc
     6661 gatcgtcgtc cagcgcggtc gcgacctgcc gccgccggtc gcgggccaga tcatggatcg
     6721 catcggcgag gtcggcgacg gctccgtcac ggtatgccgc gagcagcgcc gccgtgtcgc
     6781 tctcgcgcgg gtcggtgtcc agccccgaga cgtcggcgat gtcgaccagg agcgtctcgc
     6841 ccctgcgtcg gttgaggtgc agcccgcgtc tgggcgcgtg gacgagtttg cggacgtaca
     6901 ccttcgtcac ggtccatgcc ttgggccgga tgtccacggc gaggtcctcg acggtggcgt
     6961 cgatgctcgg gcgggcgtcc gcgtcgcggg tgttggtcga cnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn ncgggtgttg gtcgactggg tgttggtcga ctgggcggct
     7141 gttgcctcgg cggtgcggct gccgggtgcg agccggacga tcacccggcg ctcgcgcagg
     7201 ccggcgatgg ccaacacctc tcccgcgcgg ggttcgaacc ggcgcaggtt gaccacgccg
     7261 gtcgtgatca cctggctctc ctccagcgag gtcacccggg tcgtcgggac gaatacgcgt
     7321 ttgcggccag gaacctcgac gaccaggccg atgacccggg gcgtggtcgg cgtcgcggca
     7381 tacccggtgg gggtcgcggc atacccggtg ggcatggcca cgacgatgtc gttcacccga
     7441 cccacctggt ctcccagggg gtcgaacacc ggcagcccgg cgagttgggc aagaaaaacc
     7501 cgcttgggca gactcacagc gtgagcctac gcgtggccga cgtcataacg gtcgtgcgcg
     7561 gtgcgctgct agcgtcggtt ctggacgtgt tcaccgaaga gcggccggtc gccgcttcgt
     7621 gaaggagtgt catgcgccac cccaaggact tctatgcacc gttggctgtc ggcgctccga
     7681 tgccgatgcg cgcggtgccc gctcgcccgt cgcgggtcat ccacttcttc gacccgagca
     7741 acgccaagat ggcggccaag atccccgaga tggtggggac cgtcgacgtg cttctcggca
     7801 acctcgagga cgccgtcaag gccgacaaca aagaggcagc gcgggccggg ctggtcgcga
     7861 tcggcaagtc cacggatttc ggtgacctga ctcagttctg gacccggatc aactcgctgg
     7921 acagcccctg ggcgctcgat gacctcacga cgctcgtgag cgagatcggc gacaaactcg
     7981 acgtcatcat ggtgcccaag gttcagggcc ccgaggacat ccactacgtc gaccggctgc
     8041 tcgcccagct cgaggccaag gccgggctca cccgcccgat cctcgtgcac gcgatcctcg
     8101 agacggcccg aggcatggcc aacgtcgagg agatctgctt cgcctcgccg cggatgcagg
     8161 ggctctcgct cggccccgcc gacctggccg ccgaccggcg gatgaagacg acccgggtcg
     8221 gtggcgggca cccgggctat ctcgtccgcg aggacccgcc cggcggcgac atcaacggcc
     8281 cccgcgcgat cttccaacag gacctgtggc actacacgat cgcccggatg gtcgacgcct
     8341 gcgcgatggc cgggatcttc ccctactacg gcccgttcgg tgacatcaag gacgtcgtcg
     8401 cctgtgagga ccagttccgc aacgccttcc tgctgggttg tgtcggcgcc tggtcgctgc
     8461 acccggctca gatcgccatc ggcaagaagg tcttcagccc gtcggccgcc gacatcgccc
     8521 acgctcgtcg ggtcgtcgcc gccatgggag atggcaccgg cgccgtgatg ctcgacggca
     8581 agatggagga cgacgcgagc ctcaagcagt gcctggtcat gattgagctc gcggagcgcc
     8641 tcgccaagac cgaccccgag ctagccagcc tgtatgccgc cgcccccgat ccggcggccg
     8701 cgacgtcagc cggctgaccg gcatacctgc tcggcgctca tcgcaccacc cgcgcaccgc
     8761 cactcgaaac accgcacctg accactcggc cactcaagga gccaccgatg tccccagcca
     8821 agaagcacgg cacccgttac cggccgcgcc gctcggtcct ctacatgccg tcctccaacg
     8881 agcgggcgct ggagaaggcc aagagcctcc cgtgtgacgc gttgattctt gatcttgagg
     8941 acgcggtcgc gcccgatgct aaggaggccg ctcgcgaggc ggcgtgtgcc gcggtgaagt
     9001 cgggtgagta tggccgcaag gaactcatca tccgggtcaa cgcgctcggc acggactggc
     9061 actacccgga catgatggcc gcctgcaagg ccggtcctgc gggcatcctc gtgccaaagg
     9121 tcaactccgc caaggaagtt cacgacctgg tgcgcgagat ggagcgttac aagacgccga
     9181 agcacaccaa actctgggcg atgatcgaga ccccacggtc ggtcctggac gtcggccgga
     9241 tcgccgcggc ttccgaccgg ttggccgtgt tggtcatggg tacgaacgac ctggtgaagg
     9301 agttggatgc ccagcacctt cccggccggg tcccgatcgt tccggcgctc gcgctcgcgg
     9361 tgctgtcggc taaggcgtcc gggctgttca tcatcgacgg cgtctacaac gatgtgcgtg
     9421 accccgaggg tttcgctgtg gagtgcgccc aggggcggtc gatgggcttc gacggcaaga
     9481 ccctcatcca tcccgaccag gtcggcccct gcaacgaggc gttctcgccc agcgacgagc
     9541 aggtcgccga cgcgcgcggg ctcatcgatg cgttcgacgc cgcggtggcg gagcagaagg
     9601 ccgtggcgac ttacaagggc aagctcgtgg agcacttgca cgtgacgatg gccgagaagg
     9661 tgctggccac ccgagaggcc atcgacctcc tcggctgagc gccgcgccga ttgagcccgg
     9721 cgtgagcgac ctcagacgag ggcgtgctcg taggcgaaga cgacgatctg cacccgatcg
     9781 cggaggtcga gtttgcgcag gatcgccccg acgtgggtct tgaccgtcga ctcgctgacg
     9841 aagaccgtcg acgcgatctc ggcgttggac agcccgcggg cgacaccggc gaagacctcg
     9901 cgctcgcgct cggtcagctg ggagtatgcc gcgggcgtgg cgctgcgtcg ccggaagtcg
     9961 ccctccagaa ggctgccgag gtcggcgggg gagagcacgg cgttgccgag atggacggtg
    10021 cggatggctt cgcgcaggac gtcgggtgtg gcgtccttga ggagaaaacc gctggcgccg
    10081 tagcggattg ccgttgctgc ccggtcgtcg aggttgaagg tcgtcaggac gatgactcgc
    10141 actgggcggg ggcggcgggc ggcccgctcc ggagcgaaga tctgccgggt cgcctccacc
    10201 ccgtcgagct cgggcatccg gatatccatg aggacgacgt ccggctccaa cgcatcgaca
    10261 agcgccacgg cttcgcggcc gtcgctggct tcgcccacga cattgatccc cgggtcggcg
    10321 ccaacgacga ccgcgacccc cgcccggaag agttcctggt cgtcgacgag cagcacgcgg
    10381 atcggcgcgg ccgcatcggc ggaatccggg acgtctgggg actccgggtc cagttggtcc
    10441 acgtggtcca cctcattcgt ctcgtccacc cgggccacct ctgcgctcat ccgcggacct
    10501 cgaccgtcat cgcaggaccg ctcgcaccgg gagcgacgcg gtcgcgacga accaaccgtc
    10561 gtgcaggcgg gcgtccaggc tgccgcccac cgaggccagc cgacgcacca tcccgtcgac
    10621 ccccaggccg ggccgctggg gcggggcggc atcggcagca acggtattgc gcacctccag
    10681 tcgcagctga cccgcgccgt cgccggagtg tgtgctccag acctgctcga cgtggaccgg
    10741 ctcacggcgg tcgccgtgtc gcaccgcatt ggtgagcatc tcctgggcga cccggtaggc
    10801 gaccgtttcg agctcaggcg gtagcggttg ggggatgccg atgacggtcg actccacccc
    10861 gtgcccggcg tcgcgcaagc ctgtcacgag ggcgtccaac gtccccggtg gacgctgggc
    10921 tcggacggcg gcaccctcga cctgggtcga ctccagcacg gtccgcacgt cctgcagcga
    10981 ccggcgcgcc gcgtcggcga tccgatcgac cgtcgcccgg acctcgtcgg gatcctgcag
    11041 gtatgccgcg gcctgagcct gcgcgagaat caccgccagc gagtgcccga cgacatcgtg
    11101 gacgtccctg gccaggcggg cctggccagc gcgcaactcg gcgatctccc gctcccgctc
    11161 gacctgctgg tgggccaggg cggcgtgatc ctcggcgagg tgctgcaggg cgcgagaccg
    11221 gcgtgcctcg tcgatcgatc gcaccgccaa gccgagcagc cacggcgggc cgaagccgac
    11281 gagggcgagc cccagcggaa tgagggcgac ctgggtcccc accccgggcg gatccaccag
    11341 cacccgcttg agcgttgcgg catcgacgag cggcaccaaa ggcaggacga tgtcgagccg
    11401 ggtgccgacc agccagacac ccagcacacc gagcgcaagg atcgacaggc cgctgaacca
    11461 gaccgtgacg gtgcgtcccc agcgggccgc cccataggcc accagcgccg tcgtcacttc
    11521 ggtgatcagc agggtggacc cagtgcgcag gtgcgcgtag agcaggaccc acaacagcgc
    11581 cacggccgcc accggtgcgc gccggaagag cccgaccgcg accgcgagga cggcaaccat
    11641 ccaccaacga ccgattgacc gcgcactcaa ctcgaaggca cccgccgaag cgaccaggac
    11701 gccgagggcg gcgtcgggcc accagcgccg cacgtggcgg gtcactggcc cagccgtggc
    11761 ggcgtgtcgg ggaatggcac ggtgcctagt caacactgcg ccgcgacggc cgggacgcgg
    11821 ccacgctggc ggctcgcgac agcggtggtg acgagcaggt cgagcaggtc ggggtagtcg
    11881 agcccggccg ccgaggccat ccggggcagt tgggaatgcg gcgtgagccc cggcatggtg
    11941 ttcacctcgt tgaggatcgg accggcctcg gtgaggaaga agtccacacg ggccaggccg
    12001 cggcagccga gggcgtcgac gacctgcagt gcggcatcat ccagagcgtg tcggtctgcg
    12061 tcggcgagtg ccgccgggac ccggaagtcg gcgctgccgt cgtacttcgt cgtcgtgtcg
    12121 aagatgcccg accggccgat ctccaacggt ggcagcacgg tccaggtgcc gtcgggctgt
    12181 tcgatcaccc cgaggtcgat ctctcgaccg acgacgaact gctcgacgag caccgttgtg
    12241 tcgcaatgga aagccgcttt cactgccgcc ccgaggtcac ccgggcgctc gacaagggtg
    12301 accccatgac tggacccggc ccgcagtggc ttgacgacgc agggtccggt ccacgggatc
    12361 ggcccgtcgt ctgtcagcag gcgcccccgg gcggtggcca cgcccaccga ctcggcgacg
    12421 agttgggttg cccacttgtg catcccaatc gctcccgccc cgaccccgca accgacgtag
    12481 gggaccatcg cgacctcgca cagcgccgcg agggtgccgt cctcgccgtg cggcccgtgg
    12541 accgcaggga acacgacgtc gcattcctgc acggcggcga ccgcggcggc cagcggcaga
    12601 ggcgtgtccg ccagccaggc accgtcgcgc gtgatggtca ggcgcaggac gtcgtatgcc
    12661 gcctgctcca gtccgtcggc cactgctgcg gctgaggcga gcgacacctc gtgctcgctg
    12721 ctgcggccac cgccgatgac ggcaacccgg gtcatcgctg gtcccgtgag acacccgaat
    12781 accgccgcgc cacgcggccg cccaggccgg tgaggatctc gtgcggcagc gtctgcgccc
    12841 attcggccca ctcgaccagg gtcggccctt cgccgccgag gacgaccacg ggctccccga
    12901 gctcgatctc cagtcgcccg acgtcgatga ccgtctggtc catgttgacc ccaccgacga
    12961 ccgggcagcg gtggccacca accgacacct ccgcacgccc ggcagcaccg cgaggtatgc
    13021 cgtccgcgta gcccaccgga acggtgacca gtcgggtggc ccgcttcgcc cgccaggtat
    13081 ggccgtagcc gacgccggtg cctgccacga catcccgcac cgcgacgacc ggcgctctca
    13141 gcgtgacgac ctgagccagc agggtcgtcg aggaggggtc gacaccgacc aatccggccc
    13201 cgacccggct catcgtgtgc atggatcggg ggtcggtgag gcaggcggcg gtggtggcga
    13261 ggtggcggtg ctcgggagcg aggccgtgcc cagccgcagt cgtcaccgcc cagtcaaaga
    13321 cggcccggcc gcgttgtgag gctgcgtgcc ggggccgccc ggcgtgcgac aactgaccca
    13381 tgactccgac gacgtcgacc agtccggact gctgggcctg gccggcccgg gcgcagaggg
    13441 cgggccaggc cagcggcggc gacccgtcgc gggccatgcc gcagtcgacg gccaggtgga
    13501 cggacgcgac ccgaccggtg cggcgagcgg cacggcatac cgcgtcgagg tgctcgatcg
    13561 agccgacggc gaggtggact ccggcggcga ggccggcggc gaagtccccg tccacggggt
    13621 tgagccaact gaggatcggt gcggtgaggc cgtccgcgcg cagcgcgagt gcctcggcca
    13681 gcgaggtcac gccgaggccg attgccccgt gggacagcgc ggttcgcgcg atctgcgcgg
    13741 cgcccagccc gtagccgtcg gccttgacga cggcgagcag cgctggcgtt agcgccgcga
    13801 agcgccgggt gttggcgccg accgccgaca ggtcaatgtc aaggacggcg cccgcggtgg
    13861 gagcggcggg acggagggaa acgctgacgg ccgcgctcat gtctcggtgc cgacggacgg
    13921 gtgggcgggg ggaagtggca tgacctcgac gctagggacg tccccggtct ccgcacctcc
    13981 acctggagtg ccgtcggcct gcccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnccggag atagcgctcg cacggtcatg tttcctcagg tgggggagac cgccgggagg
    14161 ttttgcgcga accactcgag ctggcgtatg ccgtgtgctc gccaataggt gtcgtcgtga
    14221 ccgcctgcgt cgaaggtgac gaccgcgtca gggaccagct tggcgaaggc gcggttggcc
    14281 gcgatgaacg ggtcggaggt gccgcagtcc aggcgcaacg gcataccggc aaatgcttta
    14341 gtgcgggcga agacgtcgtg ggcgacgaag tccgcccggt cgtcgaaggc ccccggcgcg
    14401 ctcgctccgg gggaggtcca caacgccgag ctcatgcagc ccacagcgcc gatcacgtca
    14461 cgtccatagc gcgcggcgag ccagagggcg ccgtagccgc ccatcgacca gcccaacaag
    14521 cccaccggag cgggtgagac accctgggtc cgcaggacgg gcaggaggtc atcgatgacc
    14581 atggccccca cgtcggagcc gtcccggcgg gcgtgaaagt aggtcccgcc gcagtcgacg
    14641 gcagccaccg ccaccccgag gcgggcgacc tggtcgaaca acccgaggaa cgaggcgtcc
    14701 ccgacagaac cgcccttccc gtggagcgcg acgaccagcc gcgggctcgg gacgacggct
    14761 ggatcgggcc gcacgagtcg ccatcggggg ctcgcgcccg gccagtgggt ggaggtgagg
    14821 gcgccctcgg agccgagcgt ccccggtgaa ggcgtcggcg cggcagtgca tccggcgagg
    14881 acgatcgcgc cgacggcgcg cacccccacg cgcagcgccc ccctgcgggt caactccatg
    14941 ctccgagggt atgccgcccc ctggccacgt gggtcctatc gactgccgcg ccccgcgctg
    15001 tgcccgtcgg tcttatcgac gccccgaaag atcgtcgagg ttcgcggtgc tcgacgatct
    15061 ttgctcgacc tgacgaagga tcgtcgacct ttcaatggcg ccgaggacgg ctcgggaggc
    15121 ggtcgcaccg acggcgcgca cccccacgcg cagcgccccc tgcgcgtcac ctccatgccc
    15181 cggtgggtat gccgccccgt ctggcgcagg tgggtcacac aggtggtgag tctcgggtta
    15241 ctctggcaga accaacgagc cctttcggga ggtgcgtttg ctcaggttcc tgcccgccgt
    15301 cgtcgagctt gcgctgctcg tctactgcct cgtcgactgc atccagaccg aacgatcccg
    15361 agttcggacg ctgcccaagc tcggatggat cctgctcatc atcattctgc ccatcatcgg
    15421 cggagtcggc tggctcctgg ccgggcgtcc tcgacgggat gggacgggcg accacggggc
    15481 tttcgggacg gcgatccgcg ggctgggtgg cggtcctgac agcagcgccc ccaggcggcc
    15541 gctggctccg gaggacgacc cgaacttcat gcgcgggctg gatcacggca acccggagaa
    15601 ggaacgcctc ctcgaggagt gggaagccga gcgcgcgcgc cgcgaggccc aggagcctgt
    15661 ggcaccgaaa gacgccgagg acgcacctgg ggagaccccc gggtcccaag agcgccccac
    15721 tgacgccccc ggctgaccgc tcaccagcgg gaggtggccg cccctaagag ctgggcgaag
    15781 aactcgtcgg ggtcgacggc atacccgcgc cgggtgaacc acgcggccat ggtgcgggca
    15841 tcgcgctcga ggaattcgaa gccgtgcggg ttgccgatga cgtcgacgat ctgcggccag
    15901 tcgatgagga cgatccggtc gacgtcgagc aggacgttgt agggggagag gtcgccatgc
    15961 gcccacccca tctccgccag gctcagcatc gcctcgcgga cctggtcgaa cagcccggcg
    16021 agggccgtct ccggcggtcg gctgtcggcc agccgcggtg ccgccaggcc gtcggctccg
    16081 atgaactcca tgaggatctc ggtgccgtcg aactgaacgg ggtaagggac ggggaggccc
    16141 gcggtccaca acgcgccgag cgtctcgaac tctgcgaagg cccaacgccc cgagatcatc
    16201 tccttgccaa agtcggtgcg ccgggccatc gcccggttct cccgggagcg acgcacgcgc
    16261 cgaccctcca gatagccggc gtcgcgatgg aacatccgat gcttgctgtt gcggaagcgc
    16321 ttggccgcga ggaagacgtc gtgggtcggg tcgccaggca cccagcgccg aacgacgtgg
    16381 acgtcggcct ccttgccggt cttgagcaca ccgaggtcgg cgtcgacggc acccaggtcg
    16441 gtgacgaccc acgagggtcg gggtccaggc ccgtgggcgg ctccgtccca cgttgaccag
    16501 cggtcaccgt cggggggcgc gtcggggtgc gagtcgacgg cgaactccgc tggctcatct
    16561 gacgatggcg aatgtgacac gtgatgactc cgtatgccgg gtagctgact cccagcctcg
    16621 cggcataccc ccgtggtggg caacggatta tccgccgggg gtgagagcag gagtcaggtg
    16681 cggtgtgcgc cgaacttccg tgggaccgga tgtatccaga tccctggtca agcactcctc
    16741 tcacgcgaga aggtgggtcc tgtcgatctc tccctcgaca caaaggaact ggcgtgcctg
    16801 acaagagcct tggccgcggc ggacttcgcg tgagctatcc gagcacccac accgtcggag
    16861 ttccggtgcc caggaaggac cgtgacctcg gcgccgtcga cacccacgcg atcctcctgg
    16921 aagcgttgcg ggacaacgat ttcgagaagg tcgatgagtt cacgctgacc cctgaggtgc
    16981 ggcgcgatcg agacccgctg ccgcgcgacc ccggcaaact caccatggag cttgacgttg
    17041 agggcgagca ggacgccgtc gtcctggtcg agaaggacgg gtgctattcg tggcatctgc
    17101 cgacgggcga caaggctcga cggcgcgggc tgccggggga gaagcgggcg gtccgtttcg
    17161 acatcgatct ggccgtgagc gacgcggcat acgccaaccg acgcgtcgag cgcgggctcg
    17221 acgccggacc gcggacccgt ggtctgttcg gcgacctcct cgccggtgcg gtccgggtcg
    17281 tcgtcatgaa gttcgccgcg ccgttgctgg tcggtgcggc cgtcggcttc ctggagcgac
    17341 acgtcgagcc ggggatcgtc cacatcactg cgccggatcc cgccatctgg cagcgcgtcg
    17401 agcgtctgga tcagctgggt ctgccgaccg atcggccggt gcggatcctg ctgttcatcc
    17461 acggcacctt ctcctcgacg ctcagcgcgt tcgggtcaat ggcggtgtcg gacaacggtg
    17521 ctcgcttcct tgagtcggcg ctcaggcaat acgacgcggt gatcggcttc gatcacccca
    17581 ccctgtcggt cgacccgatg gtcaacgcga cggacctgct cacccgggtg tcgtcggtga
    17641 ccaacgaggt cagcttcgac gtcatcacgc acagccgcgg cgggctgacg acacgcagtt
    17701 tcgcggagta cgtcctgccc ggctcgggct ggaacgggcg ggtcgagaag gcggtcttcg
    17761 tcgccgcgac gaacaatggc actcacctgg ccgacaccga ccggtggtac gacctcatcg
    17821 atatcgcaac gaacctcacc atggtgggtg cgggtgtgct cgcggccatg ccgggtggcg
    17881 ctccggtcgc ggctgtggtt gccggagtcg tcaaaggtct cggcgcgctg gtcaaatacc
    17941 tcggggcgta tgccgtggag gaggacggag tgccggggtt ggcggcgatg cgccccggag
    18001 ggccgttcgt cactgggatc aaccagaccc agcccgggca accgaccccg ggaacgccgt
    18061 ggttcgtcgc caagagcaac ttccacgtca ccgtcggtga cgacagccat cgaccgccgg
    18121 aattccccaa ggagttggcc gtccggctcg ccgaggggct ggtggatcag gtgttccggg
    18181 gcgacaacga cctggtcgtc gactgcgagt cgatggggtc gatcgacgca gctgtcggcg
    18241 gcgggttcgt cgcggacacc tacgacttcg agtcgaacga cgtggtctac cacggcaact
    18301 acttcctgca gcccgacttc gtccggcgga tgaccgactg gctgttgtgg cgagtggagg
    18361 cagcgatggc cgagccacct gaggcggcgg ctgaaccgcc cgaggaggca gccgaaccgc
    18421 cagaggtggt tcggggcatc gacttcggcg agccgccgcc accaccggcg gcgcctccac
    18481 cgccggtgcg tggcgttgac gtgcctgctg accctggggc gcccccggcc gcccccaacg
    18541 tcgcggaagc caccctcctc gcgtcgatga cggccaagcc ctcggtcgga gtgccgaccg
    18601 cccttcgagt ggcgttgtcc cgcaaggaac tccacgtcgc cgctggtgag gtcggcgcag
    18661 ttgggggttt cgtcgctccc gccgaccgcc cgctggcggt caatgtcgcc gcctcggcca
    18721 acgcccgcgt cggcctggcc cccgaccaac tcggtgaatt cgcgaccgac agcttccaac
    18781 tccccccagg aggtggcacc tccgagttgg agttcttcgt cgaagcccaa gcggctggcc
    18841 cggtcaccgt gaccgtggtc gtccaggacg gcgtcaaatg gctcaagtct ctggccctca
    18901 cggcaacggc tggcgcgccg gtcgcgccgg tcgcggcggc ggttttcgcc gctcccggcc
    18961 cgctccaacc cgaggtgctc gcgtcggcat cgacgccgct gccggccagc gcggaccagc
    19021 ccgacttcga ggactacttc accatccagg tccaggaagc ccggttcgga gcggacacga
    19081 agttcctcta cgtcgtcagc tgcccccgac tcgggatcag cgagcggttc gagtccctgc
    19141 cgctgcgcgc ccggagcgac tacatcgcgg ccactctcgc cgacgtcaaa cgggcgtggg
    19201 acgaccacgg tgcgggggcg gcatacctgg agacgctgca ggacatcgga gccgacttgt
    19261 tcggcacgtt gttccccgaa gacctgcgcg cgctgctgtg ggccaaccgg tccaaactgg
    19321 cccccatggt cctgcgggcg gacgagcccg atgtcccgtg ggagttgatc catctcaaac
    19381 cggccaacgg cactcgccag aaggctccgg cgttcctcgg tcaactgggc atgctgcgct
    19441 ggcccttcga cggctacccg caggcgaccc tccgggctcg gcccgggcgg gtccgtgtcc
    19501 tgtgcccgga cagggcgggt ccggctgcac cgggcgccgg tgtccggcag gaggccgact
    19561 tcctcgagca acgcctcggc gcgtcctcgc tgcccggcac ggaagcggcg ctgcggaccc
    19621 tgttgcgcgg cggcgacttc gacctgctgc acttctccgg ccacggggtc gccgacccca
    19681 aggacatcgg ctcggccaaa ctcctcctca cccaggtgct cgaaggcggg ggcgcggtca
    19741 agctgcgcta cctcacggcg cgggcggtcg aacagaacgc ccggctgcgg cgcgacgacg
    19801 gatcgggacc cctcgtggtg ctcaacgcgt gccaattggg ccgtggcggt gaccaactca
    19861 cgggttcggg gggtttcgcc aaggccttcc tgtccaccgg ggcggcggcc tacgtcgcgg
    19921 ctctctggtc catccacgac actcccgctc acgacttcgt cacgtcgttc tatgaccgac
    19981 tgctcgcggg cgacacggtg gctgagtcag cggccgcagc ccgcgagaag gcccgcgcgg
    20041 ctggggacgc cacctggctc tcctacgtca tctacgcccg cccggacgcc cgactcgtca
    20101 cgcagtgagc gtcaaccact cgcaccaccc gcaccacccg tcctgtctct cccaccccac
    20161 tcctcccgtc cagccagaga aagaggtccc aaccatgacc aaccgtgcgc tctgcgtcgg
    20221 catcaaccag ttctccaacc tgccgatggc cagctggctc aacggctgcg tcaacgacgc
    20281 caacgacatg gcggcgatgt tgcgcaagcg cggttttgcg ccgcgatcgg tcacggtgct
    20341 caccgatgat caggccacca aggccaacgt gatggccgcg ttgaccaagc tcgtcgagac
    20401 ggccaaggag ggcgaccaca tcgtcttcag cttcagcagc cacgggaccc aggtccccga
    20461 cacggacggc gacgacggtc ggcccgagac cgacggcgcg gacgaggcgt tcgcctgcta
    20521 cgacatggcg cagaagggtg accagtggga ccccgacacc gtcatcatcg acgacgagtt
    20581 gcgggtgctc ttcggcaagc tccccaaggg cgtggtcatg gaggcctatc tcgacacctg
    20641 ccactccggc gacggcctgc gtgctgagga cctgctcgcc ggtcgccgcc cgcgcttcgt
    20701 cccgccgccg accctcaagg ggctgcgggt gatcgaggct gcggccacca agagcacgac
    20761 gaccgagttg gtcaaggcca tcccggccgg cggccggccg gtgctctacg ccgggtgccg
    20821 ctcagaccag acttcggccg acgcgacctt cgacgggcgt tccagcggag ccttcacgta
    20881 cttcttcctc aaggccctgg ccgccaaccc tgccggcacc cgggcggaga tccacaaggc
    20941 cctcaccaag ggtctggtgg acgcgtcgtt cagccagcgg cccactctcg aaggcccccc
    21001 gaaggccaag aaggccggcg tcggccaggc ttggtgacga gttcccgtcg cctggtaggg
    21061 cggcataccc ctggggatcc gactagggtc attcgggtat gccgcccacg gtcgaaggag
    21121 cccgcatgag ccacggcaag gacgttctcg acgcgctgcg cgacgagaca gcagccctgc
    21181 gcgagaagat ccccggtgtt tacgagggct tcgcgcacac ccacaaggcc gccctcggcg
    21241 acggcgcgct ttcgaccaag gccaaggagc tcatcgcctt cgggatcgcg gtgagcaagc
    21301 agtgcgacgg gtgcatcgcc tcgcatgcgc gcggcgcggc tcgcgccgga gccacgttgg
    21361 aggaggcggc cgaggcgatc ggcgtggcca tcctcatgaa cggtgggccg gcgacggtct
    21421 atggtccgcg cgcgtatgcc gcgttcagcg agttccacgc ccagttcgct cggttgcgcg
    21481 gcgcgcagac gccgggctga tcgaggggag tctgggggcg acgcagggtg ccgggactca
    21541 catggcggcg tcatcctgcc aacggtgacg ggcggcatac tcggcagtag tgtcctgtgc
    21601 tgttcagcag tctcctgtcc gcacgcctga acccgcgagg tagccatgtc acgactccag
    21661 acgaccgacg gtctcaccga cgaccagaag gagctgctca agctcgtcaa ggagttcgtc
    21721 gatgagcaga tcatccccgt ggcgaccgag ctggagcacc gcgacgaata tccacaggcc
    21781 atcgtcgacg gtatgaagga gatgggaatc ttcgggctga tgattcccga ggagtacggc
    21841 ggcctcggtg aatcgctgct cacctacgcc ctgtgcgtcg aggagatcgc gcgcggctgg
    21901 atgtcggtca gcggcattat caacacgcat ttcatcgttg cctacatgtt gctgcagcac
    21961 ggtaccgagg agcagaagca gcggtacctc ccgcgaatgg cgaccggcga ggtgcgcggt
    22021 gccttctcga tgagcgagcc ggccctcggg tcggatgtgt cggcgatcaa gaccaaggcc
    22081 gtcaaggacg aacagggcga tggctggacc atcaacgggc agaagatgtg gctcaccaac
    22141 ggtgggtcca gcaacctcgt cgcggtcctc gtgcgcaccg agaccggggc cgactcggtc
    22201 tataagaaca tgacgacctt cctggtcgag aaggagagcg ggttcggcga gaccgcaccg
    22261 gggctcaccg ttccgggcaa gatcgagaag atgggctaca agggagtcga caccaccgag
    22321 ttggtgttcg aggactaccg cacgaacgac gcgcagatcc tcggcggagt ccccggcaag
    22381 ggcttctacc agatgatgga cggcgtcgaa gtgggccggg tcaacgtcgc cgctcgcgcc
    22441 tgcggcctgt cgatgcgagc cttcgagctc ggcatccgct actcccagca gcgcgagacc
    22501 ttcggcaaga agatcgccga acaccagggc atcctcttcc ggctggccga catggcggtc
    22561 aaggtcgagg cgagccacca gatgatggtc aaggccgccc ggctcaagga cgccggcaag
    22621 cgcaacgacc ttgaagccgg catggcgaaa tacctcgccg ccgagaactg tgcggacgtc
    22681 gtcgagcagt ccttccggat ccacggcggc tatggctatt ccaaggagta cgagattgag
    22741 cgcctctacc gcgaggcgcc catgctgctc atcggtgagg gcacggcgga gatccagaag
    22801 atgatcatcg gccgacgcct gttggaggac tacgccctca agcggtgat
//
LOCUS       DAORPW010000025        22785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_370_length_22785_cov_744.403828, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000025 DAORPW010000000
VERSION     DAORPW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 22785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22785
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_370_length_22785_cov_744.403828"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLB86_03745"
     CDS             <1..453
                     /locus_tag="PLB86_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRC64279.1"
                     /translation="KKHRIEDAISATRAAIEEGIVAGGGTALIHAATAIDELTLTGDE
                     ATGAAIVRKAASEPLRWIAENAGLQGYVVTTKVAELPIGQGLNAATGDYEDLVKAGVI
                     DPVKVTRSALRNAASIASMILTTDTLVVEKKEEEPAPAAGGHGHGHGH"
     gene            complement(577..867)
                     /locus_tag="PLB86_03750"
     CDS             complement(577..867)
                     /locus_tag="PLB86_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRC64280.1"
                     /translation="MTEISRLPGPLEHAWTWQQTGSCRDLGPDAFFHPEGERGPARRK
                     RASAAKSVCGSCPVLRQCREHALTVREPYGVWGGLTEEERLAVYAGVLPIPA"
     gene            1206..1766
                     /gene="shbA"
                     /locus_tag="PLB86_03755"
     CDS             1206..1766
                     /gene="shbA"
                     /locus_tag="PLB86_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor ShbA"
                     /protein_id="HRC64281.1"
                     /translation="MRELAIVARSTPGSATEALLGQVRTMALRYARARLGRYGVEDAA
                     QDVAQEVCVAVLTALPTFDDRGLPFEAFVYSIAARKVADAQRRVMRSAAPMANVPEHA
                     DEQASPEDLALLRDDAGQVIALLASLPPAQREIIILRVAMGMSTEETAAALGMTAGAV
                     RVAQHRALTKLRSHYAGIDDGDGERS"
     gene            1763..2575
                     /locus_tag="PLB86_03760"
     CDS             1763..2575
                     /locus_tag="PLB86_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64282.1"
                     /translation="MTEPLRIDELGADDRLLDALAARTYDGADPIGGLLLSFARACDK
                     PSPTALTRKRRSGRRIVVSTFATAVFLASGASVAAAVSGYEPDRDDAWASSIEQWWST
                     LPGLSGGQGEVVAVPARATSASSSTSTTSLWAHDAPTRVVPPTPTPAVIVVPAETSAD
                     ASASSTESSSSAAPTGEVETPTPPTTPTAPTEPTPTPTPSTPSSASPGSTDGSSHPAG
                     PPAGKSGSTPGQNANPGGPAMAPEPQPGGRPTGGHNVPAAMLEALDLAPAAS"
     gene            2616..4136
                     /gene="guaB"
                     /locus_tag="PLB86_03765"
     CDS             2616..4136
                     /gene="guaB"
                     /locus_tag="PLB86_03765"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRC64283.1"
                     /translation="MIDSSGSDSAPFAPLGLTYDDVLLLPGETDVVPSEVDTTARLTR
                     EISIRLPLLSSAMDTVTESRMAIAMARQGGLGILHRNLSIVDQAYQVDVVKRTQTGMI
                     TNPITIGQDATLEDLDEICGQYRVSGLPVVDATNHLLGIITNRDLRFTPVAEWGTTLV
                     RDVMTPMPLITAPEGISRDAATLLLRQHKRERLPLIDDEGRLAGLITVKDFVKSEQFP
                     HASKDAQGRLMVGAAIGYFGEAWERAGTLVEAGVDVLVVDTAHGHARLLLEMVRRLKK
                     DPATAHVQVIGGNIATRAGAQALVDAGADAVKVGVGPGSICTTRIVAGVGVPQVTAVH
                     EAAKAARPAGIPVIADGGLQYSGDIAKALVAGADTVMVGSLLAGCEESPGELIFVNGK
                     QFKSYRGMGSLAAMSSRGKKSYSKDRYFQADVTSDDKIIPEGIEGQVLYRGPVGSVVH
                     QLVGGLHQSMFYVGAQTIPQLQERGRFVRITPAGLKESHPHDVQMTVEAPNYTSRS"
     gene            4581..5702
                     /locus_tag="PLB86_03770"
     CDS             4581..5702
                     /locus_tag="PLB86_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HRC64284.1"
                     /translation="MTEIEIGRGKRGRRAYSFDEIAVVPSRRTRDPEDVSVAWQIDAY
                     HFDIPVMGAPMDSVMSPSTAIALGRLGGLPVLDLEGLWTRYDDPEPLLDEIAQLDAAH
                     ATSRMQQIYAEPIRPELITERLRELRDAGVTVAGALSPKHTQAHWRTVLDAGVDLFVI
                     RGTTVSAEHVGTRAEPLNLKRFIYELDVPVIVGGASTYTAALHLMRTGAAGVLVGFGG
                     GAAHTTRRTLGIHAPMASAIADVAAARRDYLDESGGRYVHVIADGGVGTSGDIVRAIA
                     CGSDAVMLGAALARAAEAPGTGFHWGPEAHHQSLPRGERVQVGVRASLAEILFGPGRS
                     ADGTTNIIGALRRAMATTGYSDVKEFQRVEVVVAPHSHA"
     gene            5705..7315
                     /locus_tag="PLB86_03775"
     CDS             5705..7315
                     /locus_tag="PLB86_03775"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HRC64285.1"
                     /translation="MTASPAQSVAAPTTQPGGIPQSYAVPAPLVAALTRRLITGGRPE
                     LRTVVTPMTGQALYELPCSSRADVEVAIDHARGAQRAWSRLPIEHRARIIMRVHDLVL
                     DRQVQLLDLMQLESGKTRLQAFEEIADVALVARHYARAGGSDLASKRAWGAYPVLTRA
                     ETIRQPYGVIGIVSPWNYPFTLPIGDSLPALLAGNAVVLRPDPQTTLTALFGAQMLAD
                     AGLPDGVLQVVSGEGPTVGQAVVDLADYVCYTGSTEVGRAVAQTAARRLVPSSLELGG
                     KNTLYVRSDADLRRAVPGAVRASFGSAGQLCVHAERVVLHESIADAFLADFVPAVAAM
                     RLGPALEYGVDMGTLVSQRQLDRVQAHVADAVAKGAQVLVGGHARPDLGPFFHEPTVL
                     AGVTPDMACRNDETFGPVVSIYRVRSDEDAIALANDTAYGLNASIWSRDVRAARALAR
                     RIRAGTVNINDGYAAAWGASGAPMGGMGQSGLGRRHGPEGITKYTESQTIAAQHLVPI
                     APFGGLDDRQFATALTAGLKLLRRAGLR"
     gene            7318..9078
                     /gene="murJ"
                     /locus_tag="PLB86_03780"
     CDS             7318..9078
                     /gene="murJ"
                     /locus_tag="PLB86_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRC64286.1"
                     /translation="MPNAEEPQSGSSGADPQTAPPLETRRSRRESGRGNGRGAPGSGG
                     SVARNSASMALGTLGSRVLGFIRTAMLTAVIGGALAADSFTVANSLPTQVYVLINGGL
                     LSALIVPQITKAMARQDGSRDFTDRLLTLAFLVLAGVTAICLPAAPWIIDLLSANKSP
                     DFLSLTTALAYICMPQVFFYGLYSVLGQVLNAYGRFSAYAWAPAWANVIQIAGLAYFI
                     REWGQQPTLTTWTPTMVWVLAGTTTLGIVVQGLCLIVPLMRSGFRYRPRFGWRGYGFG
                     DLSRMAGWTIAALTVSQLGGLVTTRAMTVGASQAPNVAGNGIQQYAYSLYTLPHSLIT
                     VSIVTALFPAMSRAFQEGDLAGLRRWVVTGLTAPAVLVIPAATVLIALGRPMVSALFP
                     GLRVVPALGVDEPGNVALVLALMSIGILPFGITALKQRYCFARGDGWLNFWQVGVMTL
                     GNLITAVVAMVATPPEYVVAVVAAGGTISNIVGAGLFLLVARRQLDGLALRGVWFLWL
                     RLTVASGVAGLAAWAAASYIAAPTSRWLLQAIALIVGGVLFVLAFVVASRLLRIGEVT
                     EVLTRLTSRITGRLGARSAR"
     gene            complement(9088..9828)
                     /locus_tag="PLB86_03785"
     CDS             complement(9088..9828)
                     /locus_tag="PLB86_03785"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016664279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HRC64287.1"
                     /translation="MSAEGWSSPIVGVMGGLGPAATVSFLDLLVRLTKADRDQDHIDA
                     IVLQHATTPDRTAALLDPTAPDPTPALVADAQRLERMGADFLVLPCNSANIFTEPVER
                     AIGIELLSIVQETARVALALADGPIAVFATEGTIHAGTYHEAITRGGGIAWTPPRPLQ
                     ATISAIIYDQVKAGRPVDLGALHASIDVAREAGCSHVIFGCTELSVVYDREGLSERVD
                     VVDSLRTLAEATILKAGRALVERGTAPA"
     gene            complement(9825..11105)
                     /locus_tag="PLB86_03790"
     CDS             complement(9825..11105)
                     /locus_tag="PLB86_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64288.1"
                     /translation="MFTRIAEPGGGSAPEFVPVILGGHVGAYSLARAFHEGYGVRSLV
                     VTGGDTWVTDHSTIMEKVHCPDLLDEDTLVRTLTGAPFTDITVPMLLLATSDTLVEKV
                     VAVADRVGPGFRVPYVGVEVLERATRKHTFDEVAQKAGVSTPATAIIDLGGDDWHEVQ
                     APGPFPLILKATAPTAWHGVSFEGQAKVHEVASEAELQELVARIHAAGYRDKLVLQDR
                     IPGDDTGMRILTCYCDRDSNVRFASWGQTLIEEHSPGQLGVPAAILTGVNSEVVAQAT
                     RLLAELRWVGYANFDLKYDPRDDSVKFFELNPRLGRSNYYVTGTGHNAVSYYVDEYIR
                     DTLGPVGPVLESTAETIYSVVPVRTIKWALQGHPELWEKVHEVIQAGGLRNPLHYRAE
                     KDPRRWVKVALGGLSYDRKFHRYYDPLGEIGSGT"
     gene            complement(11086..12294)
                     /locus_tag="PLB86_03795"
     CDS             complement(11086..12294)
                     /locus_tag="PLB86_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRC64289.1"
                     /translation="MTVTVLHLTDREHDDFVRDHRHGHLMQTVAWGKVKEPTGWTWQR
                     VAVGRHTPEGPEVTGAALLLFRRTPVLPLALCYCPRGFVVDWADTESVNALLHEVTRV
                     AKANSAVTLRIDPAVPIDNDFVAPALRSRGFTRTDPGDDAAKALAQPAMRMVTGIGDL
                     DGLLKELPQGTRWSIRKAEKSGLVYESAGREGIDAFHDLLRTTGERDDFGVRSKGYFT
                     GLYDLLSPTDNITISLVRLDPALARAAAQKLAAESTSQLGKLRSKPHSEKQDRQIETL
                     EQSVTNCAALVAEMDAIEAVTPRGPYLAGSVFATCGSRGSYLYAASSNDYRNLRPNYL
                     MVWELMKRASAAGVATFDFGGIDASEGLEEFKRQWNPQRLEYAGEFTLRLRPLAGRLV
                     EALVRVYKNR"
     gene            complement(12358..13140)
                     /locus_tag="PLB86_03800"
     CDS             complement(12358..13140)
                     /locus_tag="PLB86_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRC64290.1"
                     /translation="MTKSDGPRVTDPAHRDGMRAFEVVLAWVESRILAGELCVGDQLP
                     PERELAKQLEVSRAAVREAVRTLQAQGVVRSAVGAGGTGGTTVTAVPAHALTRLLRLH
                     VALANFAVDDVIEARIAMERLSIRLACRNARASDLAEMRRAIAVMDDPEIDKSRFNDA
                     DTALHLAIAEAAGNRLAADFTGAIRESIRGPLLDAIRDVAGWEDLIETLRADHRRLYA
                     AIERRDADAAESLVEDHIRSAWRQLRPGVPAPMVASEVRARR"
     gene            13329..15107
                     /locus_tag="PLB86_03805"
     CDS             13329..15107
                     /locus_tag="PLB86_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HRC64291.1"
                     /translation="MIPALAITTDAYQPDLAPLGGSLVGSALIALIPLLTVFVTLGWL
                     RWKAHVAGLTAVAVAILVAVLVYRMPPGLAVLSATEGAVFGLFPIMWIVFTAIVLYQI
                     TVVSGRFEDLRATFHLISDDPRIQAIIIAFCFGGLLEALAGFGAPVAITGVMLMALGF
                     SPLRAAAVVLLANTAPVAFGAIGTPIITAGALTKIPYTEIGAYVGHQTPFIAAFVPLL
                     LVLMVDGRRGVRQTWPVAVVVGVAFAVAQWISATYISVELTDIIASLVGLAVAVVFLR
                     FWQPVGKEAAHATLAVERERDLVLAAAGSVATATGKGGGPSGAKSATAAYAVSAQELE
                     TRARGLTKGRIVMALFPYLIVIAVFSIAKLVAPVKAWLASTDLKIPWPGLDGNVLTAT
                     GAKNTSTVYTLPWLSGPGTSLLICSLITAVVYGVTMAAWGKELKDTAYKMRFAFLTVS
                     SVLALAYVMNLSGQTITVGTWIAGTGAAFAFFSPVLGWIGTAVTGSDTSANALFATLQ
                     QTAAKGAGLDPTLLVAANTSGGVVGKMISPQNLTIAATAVGLVGRESDIFRKVILWSV
                     GLIIVMCLLVGLQSTVLAWMLPEFVK"
     gene            15104..15907
                     /locus_tag="PLB86_03810"
     CDS             15104..15907
                     /locus_tag="PLB86_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRC64292.1"
                     /translation="MTASSITPTVTPAVTPTVAKGLKVALFAACYNDTMWPGTPIAVV
                     KLLERLGCRVEFPQRQTCCGQIFTNTGYAAEALPLVRGFVDTFAAYDAIVAPTGSCVG
                     AIRHQHHDLAAAAGDVALTRAVDALTPKVYDLSEFLIDVLGVTDVGAYFPHRVTFHPT
                     CHSIRVAGVGDRPQRLLQAVRGLTLVDLPHKEECCGFGGTFSLKNADVSIAMASDKAR
                     HVRETGAEVLVAGDNMCLMNMGSVLARQRAGVRVMHLAEVLASTEEDPA"
     gene            15985..17418
                     /locus_tag="PLB86_03815"
     CDS             15985..17418
                     /locus_tag="PLB86_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HRC64293.1"
                     /translation="MPKFPKAAKEALANTQQRRNLAHATGVIRAKRAGVVAELPQWED
                     LRLAGAAAKDETMTHLAAYLEQLEANLVRNGAQVHWARDAAEACQIVVDIAAAKGVDE
                     VVKVKSMATQEIELNEALEAAGIHAWETDLAELIVQLGHDRPSHILVPAIHRNRSEIR
                     EIFRREMHLVGRPAPEDLTDEPRRLAEAARLHLREKFLRAKVAVSGANFAIADTGTLV
                     VVESEGNGRMCLTLPETLISVVGIEKVLPTWQDLGTFMQLLPRSSTGERMNPYTSMWT
                     GVHPGDGPQEVHVVLIDNGRSHVLADPLGRQALRCIRCSACLNVCPVYERAGGHAYGS
                     VYPGPIGAVLNPQLRGTASDVDKSLPFASTLCGACFEACPVRIDIPSLLVHLRAQVVD
                     ESGRKSAEAKAMKAMAWMMGDYRRLEKAQKAATLGGRLLKGRTIHTMPGPFAPWTSAR
                     DVPSPPTSSFRDWWKARNTDSTTEDQR"
     gene            17415..18152
                     /locus_tag="PLB86_03820"
     CDS             17415..18152
                     /locus_tag="PLB86_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRC64294.1"
                     /translation="MTTTSLPAADSAARTAEQLRTAEQIRNDAARAAIFGRVAAAVTD
                     ISAPLGARGEAPRIEQASPGRAQTVELFIENVVDYRATVVPIAPGQEGSAIAEALQAA
                     TSVVVPSGLDPAWTAGLPDGIRIVSDDHLDNAALDGVAAVVTAATVGVATTGTIVLDH
                     GPDQGRRALSLVPDLHVCVVRASQVVHDVPEAVARLRSTVDQGASAGMPRPLTWISGP
                     SATSDIELERVEGVHGPRTLHVILVQD"
     assembly_gap    18155..18254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18255..19445)
                     /locus_tag="PLB86_03825"
     CDS             complement(18255..19445)
                     /locus_tag="PLB86_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC64295.1"
                     /translation="MADRTPQTPLGLFGTDALIDAEDRAIRDTVRAVLTAKVRPHIGE
                     WFESGTIPARELAVELGALGLLGMHLQGYGCAGTSATAYGLACVELEAVDSGVRSLVS
                     VQGSLAMFALYRFGSEEQKQEWLPRMATGEAIGCFGLTEPDFGSNPAGMRTYAARDGS
                     GDDADWVLTGTKMWITNGSVADVAVVWAQTDEGIRGFIVPTASPGFSAPAIKGKLSLR
                     ASVTSELVLDQVRLPASAMLPLAKGLSGPLSCLTEARFGIIFGALGAARDCLETTLEY
                     VATRDIFDKPLAGYQITQTKLADMTLELGKGMLLALHLGKLKDAGTLRPEQVSLGKLN
                     NVRESIAIARECRTLLGANGITLDYPVLRHANNLESVLTYEGTSEVHQLVIGQALTGQ
                     AAFR"
     gene            complement(19524..>19799)
                     /locus_tag="PLB86_03830"
     CDS             complement(19524..>19799)
                     /locus_tag="PLB86_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC64296.1"
                     /translation="AIIAFSKTIARESARHQVTVNVVCPGPTDTALFASMGGDNPKLR
                     EGLTRAIPLRRLAAPEDIANAVAFFASDEAAYITGQTLSVSGGLTMS"
     assembly_gap    19800..19899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19900..20120)
                     /locus_tag="PLB86_03835"
     CDS             complement(<19900..20120)
                     /locus_tag="PLB86_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC64297.1"
                     /translation="MRLQDKVTIVTGAGAGIGRAIARVTAAQGAIVVVTDLDGPAAEA
                     VATEIGGSASWYAVDVTDRASVAAMVAAVT"
     gene            complement(20148..21293)
                     /locus_tag="PLB86_03840"
     CDS             complement(20148..21293)
                     /locus_tag="PLB86_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC64298.1"
                     /translation="MDYGLDSEQEDFRQAVRQFATKALAPHYQRDDKTATFRREQALD
                     LAAMGLTGLRIPEAYGGQDASAVIAGIATEEVGRADFNATYVIINTALVSDIIVRNAT
                     PEQQAAWLPGIAAGTTVPCICITEPGHGTDAASLELKAIRTATGWTLHGEKTSITLGM
                     ASDRAVVLARTGGPGARGVSAFWVDLHDPTVTRSAFDDLGSRAIGRAALHFDGTPVGD
                     DDLIGAEGGGFVSVMQGFDYSRAIIGLGCIGAAEASLDEALQWSRDRIAFGQPIGTFQ
                     GVAFPLAECATYLKGARHICLEALWRKDTGLDHSAEAAMAKFWAPKLATEVIHQCLLT
                     VGHVAYSSDSPIGQRLRDVIGLEIGDGTAQVSKLVISRHLLGRDFAP"
     gene            complement(21301..22098)
                     /locus_tag="PLB86_03845"
     CDS             complement(21301..22098)
                     /locus_tag="PLB86_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC64299.1"
                     /translation="MTPFEDITYVVQDSAAIVTINRPERYNAFRAQTVEELIKAFRLA
                     WADRQVQSVILTGAGDKAFCTGGDVKQRAETGDYGPSESGMFEIGALHKMIRDIPKPV
                     IAAVNGVAVGGGHVLHVLCDVSIASETARFGQAGPRVGSFDAGFGTAYLARVVGEKKA
                     REIWFWCRMYSAQEALAMNLVNKVVPPGEVMAEAKAWADEVATKSPTAIRFLKQSFNA
                     DTDHQAGLSNLAMSALDLFTASPEGLEGAAAFAEKRTPDFNQHVNWH"
     gene            22172..>22785
                     /locus_tag="PLB86_03850"
     CDS             22172..>22785
                     /locus_tag="PLB86_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC64300.1"
                     /translation="MSSADPSREARAEARVETRRRQILQSATRIMIRTGYHEMSMQAV
                     ADDAGMSVGLIYQYFGGKQEVLRAVIVDILDTFRAEVPAAMAAAGADPEAQLAAGFAS
                     FCRVIDAKRAGTMLAYRESQTLDAAGQAEIIRLEQETIDPFRVAVEAGIATGVFRPVS
                     AELVAHNLKLAAHGWALKHWDVGARLDLTAYIDAELDLLLAAIRA"
BASE COUNT         3355 a         7910 c         7734 g         3586 t
ORIGIN      
        1 aagaagcacc gcatcgagga cgccatctcg gcgacccgcg cggcgatcga ggagggcatc
       61 gtcgccggag gcggcaccgc tctcatccac gctgcgacgg ccatcgacga gctgaccctc
      121 accggtgacg aggcgacggg tgcggcgatc gtccgcaagg ccgcctccga gccgctgcgg
      181 tggatcgccg agaacgccgg gctccagggc tacgtcgtga cgaccaaggt ggccgagctg
      241 ccgattgggc agggcctcaa cgccgccacc ggcgactacg aggacctggt caaggccggc
      301 gtcatcgacc cggtcaaggt cacccggtcc gcgctgcgca acgccgcgtc gatcgcctcg
      361 atgatcctca cgaccgacac cctggtcgtg gagaagaagg aagaggagcc cgctcccgcc
      421 gctggtgggc acgggcacgg ccacgggcac tgagccccac ggcatacctg ctggcgcgtg
      481 agccgcgccg aacgggccgc gatccccccg agggagcgcg gcccgttcgc cgtttcacca
      541 ctcggtccgg gagtggtcac atggggacgt cccgcatcaa gcggggatcg gcaggactcc
      601 tgcgtagacg gcgagccgtt cctcctcggt caggccgccc cacacgccgt agggctcacg
      661 cacggtgagg gcgtgctcgc ggcactgccg aagaaccggg cacgatccgc acaccgactt
      721 ggccgctgag gcacgcttgc gacgggccgg acctcgttca ccctctgggt ggaagaacgc
      781 gtccgggccc aagtcacggc aggagccggt ctgctgccag gtccaggcgt gctccagcgg
      841 ccctggcaat cgcgaaatct ccgtcaccgt aactccccac tgtgcgcgac gtcgtgacga
      901 ctcccgaacg actccgcccg tatgccgtcc ggtgttcgga acgctaaccc gaagcacgcc
      961 ggaggtggtc acggtgtgag tcaaggcttg ttaaagaatg ggtaagggtt cggcgaaaga
     1021 agtacagccg taaactactc atgcgacctt ggtgtcgcgt acgctcatcg acggtgggcc
     1081 gttgacgtcg atcaatcggg gaaacagtgg tgtggtctac gggtgctgca cgtcggcgcg
     1141 acgttccggt cgccgtaacg ccgcaaccgc tttctgcgat agaagagcca ggcgcgccgc
     1201 tctcgctgcg cgaactcgcc atcgtggcac gctcgacgcc cggttcggcc actgaggccc
     1261 tcctggggca ggttcggacg atggccctgc gctatgccag ggctcggctg ggtcggtatg
     1321 gcgtcgagga tgccgcgcag gatgtcgccc aggaggtgtg cgtggctgtt ctcacagctc
     1381 tgccgacctt tgacgaccgc ggtctgccct tcgaggcgtt tgtctactcc atcgccgctc
     1441 gcaaggtggc cgacgcgcaa cgccgggtca tgcgttcggc ggccccgatg gcgaacgtgc
     1501 ccgagcacgc cgacgaacaa gccagtcccg aggacctggc cctgttgcgc gacgacgctg
     1561 gccaggtcat agccctgctg gccagtctcc ctcctgccca gcgcgagatc atcatcctcc
     1621 gcgtggccat gggcatgtcg accgaggaga ccgcggccgc cctgggcatg acggccggtg
     1681 cggtccgagt cgcccagcac cgggctctca ccaagctgag atcgcactat gccggtatcg
     1741 acgatgggga cggggaacgg tcatgaccga accacttcgc atcgacgagc tcggggccga
     1801 tgaccggctc cttgacgctt tggcggcacg cacgtatgac ggcgccgacc cgatcggcgg
     1861 gctgttgctg tccttcgccc gggcctgcga caagccatcg ccgacggcgc tgacgcgcaa
     1921 gcggcgttcc ggccgacgga tcgtcgtcag cacgttcgcc acggccgtgt tccttgcctc
     1981 aggtgcgagc gtggccgctg cggtcagcgg ctacgagccc gatcgcgacg acgcctgggc
     2041 gtccagcatc gagcagtggt ggagcaccct ccccgggcta tcagggggcc agggtgaggt
     2101 ggtcgcggtg ccggctcggg cgacctccgc aagttcctca acgtcgacca cctctctgtg
     2161 ggctcatgac gccccgaccc gggtcgtgcc gcccacccca acgcccgccg tgatcgtcgt
     2221 gcccgctgag acatccgccg acgcctccgc ctcgtccacc gagtcctcgt cgtcggcggc
     2281 cccgacgggc gaggtggaga caccgacgcc cccaacgacc ccaacggccc caaccgagcc
     2341 gacgccaacg ccaacgccga gcaccccctc gtcggcgagc cccggctcca ccgacggttc
     2401 gtcgcacccc gccgggcccc ccgcgggtaa gtcgggcagc acccccgggc agaacgccaa
     2461 cccgggcggt ccggccatgg ctccggagcc ccaacccggt ggccgaccga cgggcggcca
     2521 caatgtccct gccgcgatgc tcgaggcctt ggacctcgct ccggccgcaa gttgagccga
     2581 ggggcggggc gcctcggccc cggtagactc gggcgatgat cgactcgtcc ggttctgaca
     2641 gcgcgccgtt cgctcccctg gggctcacct atgacgacgt tctgctgttg ccgggcgaga
     2701 ccgatgtcgt gcccagcgag gtcgacacca ccgcgcggct aacccgcgag atctcgatcc
     2761 ggctcccgct gttgtcctca gcgatggaca ctgtcaccga gtcacggatg gccattgcca
     2821 tggctcgtca gggcggcctc ggcatcctgc accgcaacct ctcgatcgtt gaccaggcct
     2881 accaggtcga tgtcgtcaag cgcacccaga ccgggatgat caccaacccg atcaccatcg
     2941 ggcaggacgc cacgctcgaa gacctcgacg agatctgcgg gcagtaccgc gtgtcgggtc
     3001 tgccggtcgt cgacgccacc aaccacctgc tcggcatcat caccaaccgc gacctgcgct
     3061 tcacccccgt ggccgaatgg ggcacgaccc tggtccgcga cgtcatgacg cccatgccgc
     3121 tcatcaccgc gcccgaaggc atctcccgcg acgcggcgac cctcctgctg cgccagcaca
     3181 agcgcgagcg actgccgctg atcgacgacg aggggcgcct tgccgggctt atcaccgtca
     3241 aagacttcgt caagtccgag cagttcccgc atgcctccaa agacgcccag ggtcgactca
     3301 tggtcggcgc ggcgatcggg tacttcggcg aggcctggga gcgcgccggc acgctcgtcg
     3361 aggccggcgt cgacgtgctc gtcgtcgaca ccgcccacgg acacgcccgg ctcctgctgg
     3421 agatggtccg tcggctcaag aaggacccgg cgaccgcgca cgtccaggtc atcggcggca
     3481 acatcgccac ccgagcaggc gcccaggccc tcgtcgacgc cggtgccgac gcggtcaagg
     3541 tgggggtcgg gccaggctcg atctgcacga cccgcatcgt cgccggagtc ggcgtccccc
     3601 aggtgactgc agtccacgag gcggccaagg cggctcgccc ggcggggatc cccgtcatcg
     3661 ccgacggtgg cctgcagtat tccggcgaca tcgccaaggc cctcgtcgcc ggggccgaca
     3721 ccgtcatggt ggggtcgctg ctcgctgggt gcgaggagag cccgggtgag ctcatcttcg
     3781 tcaacggcaa gcagttcaag tcctaccggg gtatggggtc gctcgcggcg atgtccagcc
     3841 gcggcaagaa gtcctactcc aaggaccgct acttccaggc cgacgtcacc agcgacgaca
     3901 agatcatccc cgagggcatc gaggggcagg tcctctatcg cggcccggtc ggctcggtcg
     3961 tccaccaact cgtcggtggc ctgcaccagt cgatgttcta tgtcggcgcc cagaccatcc
     4021 cgcagttgca ggagcgcggc cggttcgtcc ggatcacccc ggcggggctc aaggagagcc
     4081 acccgcacga cgtgcagatg accgtcgagg ctcccaacta cacctcgcgc tcgtagcccg
     4141 tcagggtgtg cgcccggggc cacaatcagg tcccgaaccc gcctgagatc gctacctgac
     4201 cgtgatgtgt tcggttgtgg gagagggggt ttggggcggg gggtcggtcg gtggtctctg
     4261 tcgccgggaa cgtcagcgtc ccggtcaccc tgacggtggt gaccgagttc gccgggttga
     4321 agccgcgtct gcgtgaggaa gccgccccga cggtgctgaa gggtttgttg gacatgggca
     4381 tcgctgacgg gccgaagggt gtccgggtcc gtccggccag ccctgttggc ggcgcacggt
     4441 gcggatggtg agttccaacg ggacttcctg tcgcgacaca gtcgacagat cacctgtcac
     4501 ggcgcaaccg agcgagacgg catacccctg ggtgtgccgc cgcgcagggg aggcgacctc
     4561 ccgcccggta gcctgggtgg gtgaccgaga tcgagatcgg ccgtggcaaa cgcggtcgcc
     4621 gggcctactc cttcgacgag attgccgtgg tgccctcgcg gcgcacccgc gaccccgagg
     4681 acgtgtcggt cgcgtggcag atcgacgctt accacttcga catccccgtc atgggggcgc
     4741 cgatggactc ggtgatgtcg ccatcgaccg ccatcgcgct cggtcgcctc ggcgggctgc
     4801 cggtcctgga cctcgagggg ctctggaccc gctacgacga ccccgagcca ctcctggacg
     4861 agatcgccca actcgacgcg gcccacgcca cgtcccggat gcagcagatc tacgccgagc
     4921 cgattcgccc tgagctcatc accgagcggc tccgggaact gcgcgacgca ggcgtcaccg
     4981 tcgccggagc gctcagcccg aagcacaccc aagcccactg gcgcaccgtg ctcgacgccg
     5041 gtgtcgacct gttcgtcatc cgcgggacga ccgtctcggc cgagcatgtc ggcacccggg
     5101 ccgaacccct caacctcaag cgcttcatct acgagctcga cgtccccgtc atcgtcggcg
     5161 gcgcgtccac ctacaccgcc gccctacacc tcatgcggac gggcgcggcc ggcgttctcg
     5221 tcggcttcgg gggaggcgcc gcgcacacga cccgtcgcac cctgggcatc cacgctccca
     5281 tggcctcggc catcgccgac gtcgccgccg cacggcgcga ctatctcgac gagtccggcg
     5341 gccgctacgt ccacgtcatc gccgacggag gcgtgggcac gtcgggcgac atcgtccggg
     5401 cgatcgcctg cggctccgac gcggtgatgc tcggtgccgc gttggccagg gccgccgaag
     5461 ccccgggcac cggattccac tggggccccg aggcacacca ccagtcgctg cctcgcggtg
     5521 agcgggtgca agtcggggtc cgcgccagcc tcgccgagat cctcttcggc cctggtcgca
     5581 gcgccgacgg cacgacgaac atcatcggag cgctccgtcg ggccatggcc acgacgggct
     5641 actccgacgt caaggagttc cagcgcgtcg aggtcgtcgt cgcccctcac agtcacgcct
     5701 gacgatgacc gcctcaccgg cccagtccgt cgcggccccc accacccagc cgggcggcat
     5761 accccagtcg tatgccgtcc cggcaccgct cgtggccgcg ctcactcggc ggttgatcac
     5821 cggcggtcgc cccgagttgc gcaccgtggt gaccccgatg accgggcagg ctctctatga
     5881 gctgccgtgc tcgtcgcggg ccgacgtcga ggtggccatc gaccatgcgc gcggtgcgca
     5941 gcgggcgtgg tcgcgactgc cgatcgagca ccgcgctcgc atcatcatgc gggtccacga
     6001 cctcgtgctg gatcgtcagg tgcagttgct cgacctcatg caactggagt ccgggaagac
     6061 tcggctgcag gccttcgagg agatcgccga cgtcgccctc gtggcgcggc actacgcccg
     6121 ggccggcggc agcgatctgg ccagcaagcg agcgtggggg gcctatccgg tcctcacgag
     6181 ggctgagacg atccgccagc cctacggcgt catcgggatc gtcagcccgt ggaactaccc
     6241 gttcacgctg ccgatcggcg acagcctgcc cgccttgctc gccggcaacg ccgtggtgct
     6301 tcggcccgac ccgcagacga cgttgacggc cctgttcgga gcccagatgc tcgccgacgc
     6361 cgggctgcct gacggtgtgc tgcaggtcgt gtcgggcgag ggaccgacgg tgggtcaagc
     6421 ggtcgtcgac ctggccgact atgtctgcta caccggctcg accgaggtcg gccgcgcggt
     6481 cgcccagacc gctgcgcgtc ggttggtgcc ctcgtcgctg gaactcggcg gcaagaacac
     6541 cctctacgtg cggtccgatg ccgacctgcg ccgggcggtc cccggcgccg tccgggccag
     6601 cttcggctcg gccggacagt tgtgcgtgca cgccgagcgc gtggtgctgc acgagtcgat
     6661 cgccgacgcg ttcctcgccg acttcgtgcc ggcggtcgcc gcgatgcggc tgggcccggc
     6721 gctggaatac ggcgtcgaca tgggcaccct cgtgtcgcaa cgccaactcg accgggtcca
     6781 ggcccacgtc gccgacgcgg tcgccaaggg tgcccaggtg ctcgtcggcg gccacgcccg
     6841 acccgacctg ggcccgttct tccatgagcc gaccgtgttg gccggcgtca cgcccgacat
     6901 ggcctgccgc aacgacgaga ccttcggccc ggtggtgtcc atttaccggg tgcgctcgga
     6961 cgaggacgcg atcgccctcg ccaacgacac ggcatacggg ctcaatgcgt cgatctggag
     7021 ccgcgacgtc cgcgccgcgc gcgccctggc ccgccggatc cgcgcgggca ccgtcaacat
     7081 caatgatggg tatgccgcgg cctggggcgc gtccggcgct cccatgggcg ggatggggca
     7141 gtccgggctt gggcgtcggc atgggcccga agggatcacg aagtacaccg agagccagac
     7201 catcgccgct cagcacctcg tgccgatcgc gccgttcggc ggtctggacg atcggcagtt
     7261 cgccactgcg ttgaccgcgg gcctcaaact cctgcggcgc gccgggctgc ggtgaccgtg
     7321 ccgaacgccg aggaaccgca gtcggggtcc tcgggcgctg atccacagac agcgccaccg
     7381 ctcgaaaccc gacgcagtcg gcgcgagagt ggccgcggga acggtcgcgg tgcccccggc
     7441 agcggtgggt cggtcgcccg gaacagcgcc tcgatggcgc tcggcacgct cggctctcgg
     7501 gtcctggggt tcattcgcac ggcgatgctc acggcggtga tcggcggcgc cctcgccgca
     7561 gactcgttca ccgtcgcgaa ctcgctgccg acgcaggtct acgtcctcat caacggcggt
     7621 ctgctctcgg cgctcatcgt cccgcagatc accaaggcga tggcccggca ggacgggtcg
     7681 cgggacttca ccgaccgatt gctcacgttg gcgttcctcg tcctggccgg ggtcaccgcc
     7741 atctgcctgc cggcggcgcc ctggatcatc gacctgctca gtgccaacaa aagcccggac
     7801 ttcttgtcgc tgacgacggc gctcgcctat atctgcatgc cccaggtgtt tttctacggg
     7861 ctctattcgg tgctcgggca ggtcctcaac gcctacgggc ggttctcggc gtatgcgtgg
     7921 gcaccggcct gggccaacgt catccagatt gccgggctcg cctatttcat ccgggaatgg
     7981 ggacaacaac cgactctgac cacgtggacg cccaccatgg tgtgggtgct ggccgggacg
     8041 accaccctgg gcatcgtcgt gcaagggctc tgcctgatcg ttccgctgat gcgcagcggg
     8101 ttccgctacc ggccacgatt cggttggcgc ggttacggtt tcggggacct gtcccggatg
     8161 gcggggtgga cgatcgcggc gctcacggtg tcgcaactcg gggggttggt gacgactcgg
     8221 gcgatgacgg tcggtgccag ccaagccccg aacgtggccg ggaacggcat ccagcagtac
     8281 gcctacagcc tctacaccct gccgcactca ctcatcacgg tctcgatcgt cacggcgctg
     8341 ttccccgcca tgtcgcgggc cttccaggag ggcgacctcg cgggcctgcg acggtgggtg
     8401 gtcacggggc tgacggctcc cgcggtgctc gtcatcccgg cggccaccgt gctcatcgcc
     8461 ctggggcgcc cgatggtgtc cgcccttttc cctgggctgc gagtcgtccc tgcgctcggc
     8521 gtggatgagc ccggcaacgt ggccctggtg ctggccctca tgtccatcgg catcctgccg
     8581 ttcggcatca ccgccctcaa acagcgctac tgcttcgcgc gcggcgacgg ctggctcaac
     8641 ttctggcagg tcggggtgat gaccctcggc aacctcatca ccgcagtggt cgccatggtg
     8701 gccacgcctc ccgagtatgt cgtcgccgtc gtggccgccg gtggcaccat ctcgaacatc
     8761 gtcggtgcgg ggctgttcct gttggtggcc cggcgacaac tcgacggtct ggccctgcgg
     8821 ggggtctggt tcctctggct gcggctgacg gtggcgtcgg gtgttgccgg cctcgcagcg
     8881 tgggcggcgg cgtcctacat cgcggcgccc acgagtcgat ggctcctgca ggccatcgcc
     8941 ctgatcgtcg gcggcgtcct cttcgtcctg gccttcgtgg tcgcctcgcg cctgctgcgc
     9001 atcggcgagg tcaccgaggt cctcacccgt ttgacctccc gaatcaccgg ccggctcgga
     9061 gcccgttctg cgcggtgact gagccgatca ggcgggtgcg gtgccgcgct cgacgagggc
     9121 tcgcccggcc ttgaggatcg tcgcctcggc gagggtccgc agcgagtcga cgacgtccac
     9181 gcgctcggac aggccctcgc ggtcatagac cacggacagt tcggtgcagc cgaagatgac
     9241 gtgcgagcaa ccggcctctc gggcgacgtc gatgctggca tgcagcgcgc ccaggtcgac
     9301 cggccgaccg gccttgacct ggtcatagat gatcgcggag atcgtggcct gcagcgggcg
     9361 tgggggagtc cacgcgatcc ccccgccacg cgtgatggcc tcgtggtagg tgccggcgtg
     9421 gatcgtcccc tcggtggcga agacggcgat gggcccgtca gccagggcca gggcgaccct
     9481 ggcggtctcc tgcacgatgg acagcaactc gatgccgatg gcgcgttcca ccggttcggt
     9541 gaagatgttc gccgagttgc acggcaggac caagaagtcg gcgcccatcc gctccaaccg
     9601 ctgggcgtcg gcgaccaacg ctggcgtcgg gtccggggcg gttgggtcga gcagggccgc
     9661 cgtgcggtcg ggtgtcgtgg cgtgctgcag gacgatggcg tcgatgtggt cctgatcgcg
     9721 gtcggccttc gtcagccgga cgagcaggtc gaggaacgag accgtcgctg cggggccaag
     9781 gccacccatc accccgacga tggggctgga ccagccctca gcgctcacgt gccgcttccg
     9841 atctctccga gcgggtcgta gtaacggtgg aacttgcggt cgtagctgag gccgccgagg
     9901 gcgaccttga cccatcgccg cgggtccttc tcggcccggt agtgcagcgg gttgcgcagg
     9961 cccccggcct ggatgacctc gtggaccttc tcccacaact cggggtgacc ctgcagcgcc
    10021 cacttgatgg tgcggaccgg gacgaccgaa tagatggtct cagcggtgga ctcgaggacg
    10081 gggccgacgg ggccgagggt gtcgcggatg tactcgtcga cgtagtagga caccgcgttg
    10141 tggccggtcc ccgtgacgta gtagttcgac cggcccaacc gggggttgag ctcgaagaac
    10201 ttcaccgagt cgtcgcgcgg gtcgtacttg aggtcgaagt tcgcatagcc gacccaccgc
    10261 aactccgcca acaaccgcgt cgcctgggcg accacctcgc tgttgacgcc ggtgagaatg
    10321 gctgcaggga cgcccaactg gccgggggag tgctcctcga tgagggtctg cccccaggac
    10381 gcgaaccgca cgttggagtc gcggtcgcag tagcacgtga ggatccgcat gcccgtgtcg
    10441 tcgccgggga tccggtcctg caggaccagc ttgtcgcggt acccggccgc gtggatgcgc
    10501 gccaccaact cctgcaactc ggcctcggag gccacctcgt gcaccttggc ctggccctcg
    10561 aaactcaccc catgccacgc cgtcggcgcg gtcgccttaa ggattagcgg gaacgggccg
    10621 ggtgcctgca cctcgtgcca gtcatcgccc cccaggtcga tgatggcggt cgccggcgtg
    10681 ctgaccccgg ccttctgggc cacctcgtcg aacgtgtgct tgcgggtggc ccgctccaac
    10741 acctcgacgc cgacgtaggg cacccggaac ccggggccca cccgatcagc gacggcgacg
    10801 accttctcca cgagggtgtc cgaggtcgcg agcagcaaca tcggaacggt gatgtccgtg
    10861 aacggcgccc ccgtgagggt gcgcacaagg gtgtcctcgt cgagcaggtc ggggcagtgg
    10921 accttctcca tgatggtgga gtggtccgtg acccaggtgt cgccgccggt gacgacgagc
    10981 gaccgcaccc cataaccctc gtggaaggcc cgagccagcg aataggcgcc gacgtggcca
    11041 ccaaggatga cggggacgaa ctcgggagcg gacccgccgc cgggttcagc gattcttgta
    11101 aacacggacc aacgcctcca cgagccgacc tgccagcggt cgcagccgca gggtgaactc
    11161 cccggcgtac tccaaccgct gcgggttcca ttgccgtttg aactcttcca gaccctcgga
    11221 cgcgtcgatg ccaccgaagt cgaaggtcgc gacgcccgcg gcgctcgcgc gcttcatcaa
    11281 ctcccacacc atgaggtagt tgggccgcag gttgcggtag tcgttggacg acgcggcata
    11341 caggtaactg ccgcgcgagc cacacgtggc gaagaccgac cccgcgaggt agggcccgcg
    11401 cggggtgacg gcctcgatcg cgtccatctc ggccacgagc gccgcgcagt tggtgacgct
    11461 ctgctcaagc gtttcgatct ggcggtcctg cttctccgaa tggggcttgc tgcgcagctt
    11521 gcccagctgg ctggtcgact cggccgccaa cttctgggcg gccgcccgcg ccagagcggg
    11581 gtcgaggcgc accagcgaga tggtgatgtt gtcggtgggg gacagcaggt cgtagagccc
    11641 ggtgaagtag cccttgctgc ggaccccgaa atcgtcgcgc tcgccggtcg tgcgcagcag
    11701 gtcgtggaag gcgtcgatgc cctcgcgtcc ggccgactca tagaccagac cagacttctc
    11761 tgccttgcgg atgctccagc gggtgccctg ggggagctcc ttgagcaacc cgtcgaggtc
    11821 gccgatcccg gtcaccatcc gcatggcggg ctgggcgagg gccttcgccg cgtcgtcgcc
    11881 cgggtcggtg cgcgtgaagc cgcgcgagcg cagcgccggc gcgacgaagt cgttgtcgat
    11941 cggcaccgcc gggtcgatcc gcagggtcac cgccgagttg gccttggcca cccgggtcac
    12001 ctcgtgcagg agcgcgttga cgctctcggt gtcggcccag tccaccacga agccgcgcgg
    12061 gcagtagcac agggccaacg gcaggaccgg ggtgcgccgg aagagcagca gcgccgctcc
    12121 cgtgacctcg ggtccctcag gggtatgccg tcccaccgcc acccgctgcc aggtccagcc
    12181 cgtgggttcc ttgaccttgc cccacgccac ggtctgcatg agatggccgt ggcggtggtc
    12241 gcggacgaag tcgtcatgct cgcggtcggt gaggtgcagt accgtcacag tcacgaggca
    12301 ctccttcgcc ctggtgcgat ccgatcgcag ggcaccgtcc aggtcatccg ggagagtcta
    12361 ccgtcgcgct cgcacctcac tcgcgaccat gggcgccggg acgccggggc gcaactgtcg
    12421 ccaggcagag cggatgtggt cctcgaccag cgactcagcg gcatcggcgt cgcggcgttc
    12481 gatcgcggca tacaggcgcc ggtggtcggc gcgcagggtc tcgatgaggt cctcccagcc
    12541 ggcgacgtcg cgaatcgcgt cgagcaaggg gccccggatg gactcccgaa tggctcccgt
    12601 gaagtcggcg gccaaccggt tgccggcggc ctcggcgatc gccaggtgca gggccgtgtc
    12661 ggcgtcgttg aagcgcgact tgtcgatctc cggatcgtcc atgacggcga tggctcggcg
    12721 catctcggcc aggtcggagg cccgggcgtt gcggcaggcc aagcgaatac tcaaccgctc
    12781 catagcgatc cgagcctcga tgacgtcgtc aacggcgaag ttcgccagcg ccacgtgcaa
    12841 ccgcaacagc cgggtgaggg cgtgggcagg gaccgcggtg acggtggtcc ctccggtgcc
    12901 gccggcacct accgcggagc ggacgacgcc ttgggcctgc aaggtccgca cggcctcacg
    12961 gaccgcggcc cggctcacct caagttgctt ggccaactca cgctccgggg gaagttggtc
    13021 accgacacac aactcaccgg cgaggatccg gctctcgacc caggcgagga ccacctcgaa
    13081 cgcccgcatc ccgtcgcggt gcgccgggtc ggtgacccga ggtccgtcgg acttggtcat
    13141 acccgtgcct cctgcctgcg agcacccgtc ccgcgactgg gcccttgacg tcctaatggt
    13201 cagaccttac cctagggaca accctaattg gtcagaccac cctccagcgt gactcgagaa
    13261 tggtcagacc acttggtcga ccgagccggt gtcgtcccga tcgaacccag tcagaggaga
    13321 tcgcccgcat gatccccgcc ctcgccatca ccacggatgc ctatcaaccc gacctcgcac
    13381 cgctgggggg aagcctcgtc ggctccgccc tcatcgccct cattccgttg ctgaccgtct
    13441 tcgtcaccct ggggtggctg cggtggaagg cgcacgtggc cgggctcacc gccgtggccg
    13501 tcgccatcct cgtggccgtt ctcgtctatc ggatgccgcc gggcctcgcc gtgctctcgg
    13561 ccaccgaagg cgccgtcttc ggcctcttcc ccatcatgtg gatcgtcttc accgcgatcg
    13621 tcctctacca gatcaccgtg gtcagcggcc ggttcgagga cctccgggcc accttccacc
    13681 tcatctccga cgacccacgg atccaagcga tcatcatcgc cttctgcttc ggcggcctcc
    13741 ttgaggccct cgctggcttc ggcgccccgg tcgccatcac cggcgtcatg ctcatggccc
    13801 tgggcttcag tccactgcgc gccgcggccg tcgtcctgct cgccaacacc gcacccgtgg
    13861 ccttcggcgc catcggcacc ccgatcatca ccgcgggcgc cctgaccaag atcccctaca
    13921 ccgagatcgg cgcctatgtc ggacaccaga ccccgttcat cgccgcgttc gtcccgctgc
    13981 tgctcgtcct catggtcgac gggaggcggg gagtgcgcca gacctggccg gtcgccgtgg
    14041 tcgtcggcgt cgccttcgcc gtggctcagt ggatcagcgc cacctacatc tcagtcgaac
    14101 tcaccgacat catcgcctcg ctcgtcggcc tcgcggtcgc cgtcgtgttc ttgcggttct
    14161 ggcagccggt cggcaaggag gccgcccacg cgaccctggc cgtggagcgg gagcgcgacc
    14221 tcgtgctcgc cgctgccggt tcggtcgcca ccgcgaccgg gaagggcggc ggtcccagcg
    14281 gcgccaagag tgcgaccgct gcgtatgccg tgtccgctca ggagttggag acccgcgctc
    14341 gcggcctgac caagggccgg atcgtcatgg cgctcttccc ctacctcatc gtcatcgccg
    14401 tcttctcgat cgccaagctc gtcgctccgg tcaaggcctg gctggccagc accgacctca
    14461 agatcccctg gccaggcctg gacggcaacg tcctcacggc caccggagcc aagaacacct
    14521 cgacggtcta cacgctgcct tggctctcgg gccccggcac gagcctgctg atctgttcgc
    14581 tcatcaccgc tgtcgtctat ggcgtgacca tggcggcctg gggcaaggaa ctcaaggaca
    14641 cggcatacaa gatgcggttt gcgttcctca ctgtgtcctc ggtcctcgcc ctcgcctacg
    14701 tgatgaacct gtcgggtcag acgatcaccg tcggcacgtg gatcgccggg accggggccg
    14761 ctttcgcctt cttctcgcca gtcctcgggt ggatcggcac cgcggtcacg ggctcggaca
    14821 cgagcgccaa cgcgctgttc gccaccctcc aacagacggc cgccaagggt gccggcctcg
    14881 acccgaccct gcttgtcgcc gccaatacct caggcggtgt cgtgggcaag atgatcagcc
    14941 cacagaacct caccatcgca gcaaccgcgg tcggcctggt cggccgcgag tccgacatct
    15001 tccgcaaggt catcctgtgg agtgtcgggc tgatcatcgt catgtgtctt ctcgtcggcc
    15061 tgcaatcaac cgtgctggcc tggatgctcc cggagttcgt caaatgaccg catcatcaat
    15121 cacccccacc gtcaccccgg ccgtcacacc gacggtcgcc aagggtctca aggtcgccct
    15181 cttcgccgcg tgttacaacg acacgatgtg gccaggcacc ccgatcgccg tggtcaagct
    15241 ccttgagcgg ctggggtgcc gagtggagtt cccgcagcgg cagacctgct gcggtcagat
    15301 cttcaccaac acggggtatg ccgccgaagc gctcccgctc gtgcgcggct tcgtcgacac
    15361 gtttgcggca tacgacgcca tcgtcgcccc gaccgggtcg tgcgtcggcg cgatccgcca
    15421 ccagcaccac gacctggccg ctgcggcggg cgatgtcgcc ctcacccgcg cggtcgatgc
    15481 gctgacgccc aaggtctatg acctgtcgga gttcctcatc gatgtcctcg gggtcaccga
    15541 tgtcggggcc tacttcccac atcgggtgac cttccatccc acgtgccact cgatccgggt
    15601 cgccggggtg ggtgaccggc cgcaacggct gctgcaggcg gtgcgtgggc tgaccctcgt
    15661 cgacctgccg cacaaggagg agtgctgtgg cttcggcggg acgttctcgc tgaagaacgc
    15721 cgacgtgtcg atcgcgatgg ccagcgacaa ggcgcggcac gtccgggaga ccggtgccga
    15781 ggtgctcgtc gccggcgaca acatgtgcct gatgaacatg ggcagcgtgc tggctcgcca
    15841 gcgggccggg gtgcgggtca tgcacctggc cgaagtgctc gcctcgaccg aggaggaccc
    15901 ggcatgacga ccacctaccc gagcgggccg acctcggcac cgacctctgg gccgctgcaa
    15961 gcgacgacgg cgaccttcat cgggatgccg aagttcccca aggcggccaa ggaggcgctg
    16021 gcgaacaccc agcagcggcg caacctcgcc cacgcgacgg gggtgatccg ggccaagcgg
    16081 gccggggtcg tcgccgagtt gccgcagtgg gaggacctgc gcctggccgg cgcggcggcc
    16141 aaggacgaga cgatgaccca tctcgcggca tacctcgagc agttggaagc caacctcgtg
    16201 cgcaacgggg cgcaggtgca ctgggcccgc gacgcggcgg aggcctgcca gatcgtcgtc
    16261 gacatcgctg cggccaaggg ggtcgacgag gtcgtcaagg tcaagtcgat ggcgacccag
    16321 gagatcgagc tcaacgaggc cctcgaagcc gccgggatcc acgcctggga gaccgacctc
    16381 gccgagctca tcgtccaact cggtcacgac cggccgtcgc acatcctcgt cccggcgatt
    16441 caccgcaacc gctcggagat ccgcgagatc ttccggcgcg agatgcacct ggtgggacgc
    16501 ccggctcctg aggacctcac cgacgagccg cgccggctcg ccgaggcagc tcgcctgcac
    16561 ctgcgggaga agttcctccg ggcgaaggtt gccgtgtcgg gggccaactt cgcgatcgcc
    16621 gacacgggca ccctcgtcgt cgtcgagtcg gagggcaacg gccgaatgtg cctcaccctt
    16681 cccgagacgc tcatctcggt ggtcgggatc gagaaggtgc tgccgacctg gcaggacctc
    16741 gggaccttca tgcagctgct cccgcggtcc tcgaccgggg agcggatgaa cccctacacc
    16801 tcgatgtgga ccggagtgca tcccggcgac gggccgcagg aggtccacgt cgtcctcatc
    16861 gacaacggac gctcgcacgt gctcgccgac cccttgggcc ggcaggcgtt gcgctgcatc
    16921 cgctgctcgg cctgcctcaa cgtgtgcccc gtctatgagc gggccggcgg gcacgcgtac
    16981 ggctcggtct atcccgggcc aatcggcgcc gtcctcaacc cgcaactgcg tggcacggcg
    17041 agcgatgtcg acaagtcgtt gccgttcgcg tcgacgttgt gcggtgcctg cttcgaggcc
    17101 tgcccggtgc ggatcgacat cccgtcgctg ttggtccacc tgcgggcgca ggtcgtcgac
    17161 gagtcgggtc gaaagtctgc ggaggccaag gcgatgaagg cgatggcctg gatgatgggc
    17221 gactatcgcc gactcgaaaa ggcccagaag gcagcgacat tgggcgggcg gctgctcaag
    17281 ggccgcacga tccacaccat gcccggcccg ttcgcaccct ggaccagcgc acgggacgtg
    17341 ccgtccccac ccaccagctc cttccgggac tggtggaagg cgcgaaacac ggattccacg
    17401 acggaggacc agcgatgacg acgaccagcc ttccggccgc cgacagtgcg gctcgcaccg
    17461 ccgaacagtt gcgcaccgcc gaacagatcc gcaacgacgc ggcgcgggcg gccatcttcg
    17521 gtcgagtcgc ggccgcagtc accgacatct cggctccgtt gggtgcccgc ggcgaggctc
    17581 ctcgcatcga gcaggcgtcg ccggggcggg cgcagacggt ggagttgttc atcgagaacg
    17641 tcgtcgacta ccgggccacc gtcgtcccga tcgcgccggg gcaggagggc tcggccatcg
    17701 ccgaggccct gcaggcggcc acctcggtgg tcgttccgtc cgggctcgac ccggcctgga
    17761 ccgcggggct gccggacggc atacgcatcg tctccgatga ccacctcgac aacgcggccc
    17821 tcgacggggt ggcggcggtc gtgactgccg cgaccgtcgg cgtggcgacg acgggcacga
    17881 tcgtcctcga ccatggtccc gaccaggggc gccgggcgct ctcactcgtg cccgacctgc
    17941 acgtgtgcgt tgtgcgtgcg tcgcaggtcg tgcacgacgt gcccgaggcg gtggcgcggc
    18001 tgcggtccac ggtggatcaa ggcgcgtcag ccggtatgcc gcgtccgctc acctggatct
    18061 cggggccgag cgcgaccagc gacatcgagt tggagcgggt cgaaggggtc cacgggccgc
    18121 ggaccttgca cgtgatcctc gtgcaggact gagcnnnnnn nnnnnnnnnn nnnnnnnnnn
    18181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18241 nnnnnnnnnn nnnntcagcg gaaggcggcc tggccggtca gcgcctgccc gatgacgagc
    18301 tggtggacct cgctcgtgcc ttcgtaggtg agcaccgact cgaggttgtt ggcgtgccgc
    18361 agcacggggt agtcaagcgt gattccgttg gcgcccaaca gggttcggca ttcacgggcg
    18421 atcgcgatcg actcccgcac gttgttgagc ttgccgaggc tgacctgctc ggggcgcagc
    18481 gtgccggcgt ccttgagctt gccgaggtgc agggcgagga gcattccctt gcccaactcg
    18541 agcgtcatgt cggcgagctt ggtctgggtg atctgatagc ccgccagcgg cttgtcgaag
    18601 atgtcccggg tggcgacata ctcgagtgtc gtctcgaggc agtcgcgggc cgcgccgagg
    18661 gcgccaaaga tgatcccgaa ccgggcctcg gtgaggcacg acagcgggcc ggagagaccc
    18721 ttggccagcg gcagcatcgc cgacgcaggc agccgcacct ggtcgaggac caactccgag
    18781 gtgaccgacg cgcgcaacga caacttgccc ttgatcgcgg gagccgagaa gcccggcgag
    18841 gcggtcggca cgatgaaccc gcgaatcccc tcgtcggtct gcgcccagac cacggcgacg
    18901 tcggccaccg acccgttggt gatccacatc ttggtgcccg tgagcaccca gtccgcgtcg
    18961 tcgccggagc cgtcgcgggc ggcatacgtg cgcatgcccg ccgggttgga gccgaagtcg
    19021 ggctcggtga gcccgaagca accgatcgcc tcgccggtag ccatccgggg caaccactcc
    19081 tgcttctgct cctcgctgcc gaagcgatag agcgcgaaca tcgccagcga tccctgcacc
    19141 gacacgaggg agcgcacgcc cgagtcgacc gcctccagct cgacacacgc gagcccgtat
    19201 gccgtggcgc tcgtccccgc gcagccgtag ccctgcaggt gcatccccaa caggcccaac
    19261 gcgccgagct cgacggccag ctcgcgggcc gggatggtgc ccgactcgaa ccactcgccg
    19321 atgtgcggac gcacctttgc cgtgagcacg gcccggacgg tgtcgcggat ggcccggtcc
    19381 tcggcgtcga tgagagcgtc ggtgccgaac aatccgaggg gggtctgggg ggtgcggtcg
    19441 gccatgatcg ggcttcctgt tccggtggct gggcttgctc gttcgggtgg tgggcttggt
    19501 cgttctgtca gtatgccgcg tgctcagctc atcgtcaggc cgccggaaac gctgagggtc
    19561 tggccggtga tgtaggcggc ctcgtccgag gcgaagaagg cgacggcgtt ggcgatgtct
    19621 tcgggagcag ccaagcggcg caacgggatt gcccgggtga gaccctcgcg cagcttgggg
    19681 ttgtcgccgc ccatcgaggc gaacaacgcg gtgtcggtgg ggcccgggca gacgacgtta
    19741 acagtcacct ggtggcgggc cgactcgcgg gcgatggtct tggagaaggc gatgatcgcn
    19801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tgacggcggc gaccatggcg
    19921 gcgaccgatg cgcggtcggt gacgtccacg gcataccaac tggcggagcc gccgatctcg
    19981 gtcgcgacgg cttcggcggc gggaccgtcg aggtcggtga cgacgacgat cgcgccctgg
    20041 gcggcggtga cccgggcgat ggcgcggccg atacccgcac cggctccggt gacgatggtg
    20101 accttgtcct gcaagcgcat ggggagagtc ctttcggtgg ggacgggtca gggcgcgaag
    20161 tcgcggccga gcaggtggcg ggagatgacg agcttggaga cctgagcggt gccgtcaccg
    20221 atctcgaggc cgatgacatc gcgcagccgc tggccgatgg gggagtcgct ggagtaggcg
    20281 acgtggccga cggtgagcag gcattggtgg atgacctcgg tggcgagttt gggggcccag
    20341 aacttcgcca tcgccgcctc ggcgctgtgg tcgaggccag tgtccttgcg ccacaacgct
    20401 tccaggcaga tgtgccgcgc gcccttgaga taggtggcgc actcggccag ggggaaggcc
    20461 accccttgga aggtgccgat cggttggccg aaggcgatcc ggtcgcgcga ccactgcagc
    20521 gcctcgtcga gcgacgcctc ggccgcgccg atgcagccca ggccgatgat cgcccgggag
    20581 tagtcgaagc cctgcatgac cgacacgaag ccgccgccct cggcgccgat gaggtcgtcg
    20641 tcgccgactg gggtgccgtc gaagtgcagt gcggcgcgcc cgatggcccg gctgccgagg
    20701 tcgtcgaagg cggagcgggt gactgtcggg tcgtgcaggt cgacccagaa ggcgctgacg
    20761 ccgcgcgctc cggggccgcc agtgcgggcc aggacgaccg cgcggtcgga ggccatgccc
    20821 agggtgatcg aggtcttctc gccgtgcaac gtccacccgg tcgcggtgcg aatcgccttg
    20881 agctccaggc ttgctgcgtc ggtgccgtgg ccgggctcgg tgatgcagat gcacgggacc
    20941 gtggtgccgg ccgcgatccc gggcagccac gcggcctgct gctcgggcgt cgcgttgcgg
    21001 acgatgatgt cgctgacgag cgccgtgttg atgatgacgt aggtcgcgtt gaagtcggcc
    21061 cggccgacct cctcggtggc gatgccggcg atgacggcgc tcgcgtcctg gccgccgtaa
    21121 gcctccggga tgcgcaggcc ggtcaggccc atggccgcca ggtcgagggc ctgctcgcgg
    21181 cggaaggtcg cagtcttgtc gtcccgctgg tagtggggag cgagtgcctt ggtcgcgaac
    21241 tggcggaccg cctgtcggaa gtcctcctgc tcgctgtcga gcccgtagtc catctgcgcc
    21301 tcagtgccag ttgacgtgct ggttgaagtc gggggtgcgc ttctcggcga aggcggcagc
    21361 gccctccagg ccctcggggg aggcggtgaa caggtcgagc gccgacatgg cgaggttgga
    21421 caggcccgcc tggtggtcgg tgtcggcatt gaacgactgc ttgaggaagc ggatcgccgt
    21481 cgggctcttg gtcgccacct cgtcagccca cgcctttgcc tccgccatga cctcgccggg
    21541 aggcacaacc ttgttgacga ggttcatcgc cagggcctcc tgggcggagt acatccggca
    21601 ccagaaccag atctcgcgcg ccttcttctc gcccacgacg cgcgcgaggt atgccgtgcc
    21661 gaaccccgcg tcgaaggacc ccacccgggg gccggcctgg ccgaagcggg cggtctcgct
    21721 ggcgatcgac acgtcgcaca ggacgtgcag gacgtgtccg ccgccgacgg ccacgccgtt
    21781 gaccgccgcg atgacgggct tggggatgtc gcgaatcatc ttgtgcagtg cgccaatctc
    21841 gaacatcccc gactcgctcg ggccgtagtc gccggtctcg gcgcgttgct tgacgtcgcc
    21901 gccggtgcag aacgccttgt ccccggcgcc ggtgaggatg accgactgga cctggcggtc
    21961 ggcccaggcc aaccggaacg ccttgatgag ttcttcgacg gtctgggcgc ggaaggcgtt
    22021 gtagcgctcc gggcggttga tcgtcacgat cgccgccgag tcctgcacga cgtaggtgat
    22081 gtcctcgaac ggcgtcatcg tcggttctcc tggctccgtg ctgaacgact attcattgaa
    22141 tggtcgttca atcacaggta tgctgtcaag ggtgagctca gcggacccgt cccgcgaggc
    22201 ccgggccgag gctcgggtcg agacccgtcg gcggcagatc ctgcagtcgg cgacccggat
    22261 catgatccgc accggctatc acgagatgtc gatgcaggcc gtggccgacg acgccgggat
    22321 gagcgtgggg ctgatctatc agtacttcgg cggcaagcag gaggtgttgc gggccgtcat
    22381 cgtcgacatc ctcgacacct tccgggccga ggtgccggcg gccatggcgg cggccggcgc
    22441 tgatccggag gctcaactgg cagccgggtt cgccagcttc tgccgggtca tcgacgccaa
    22501 gcgggccggg acgatgctgg cctatcgcga gagccagacc ctcgatgccg ccgggcaggc
    22561 cgagatcatc cgcctcgagc aggagacgat cgatccgttc cgggtcgccg tcgaggcggg
    22621 catcgctaca ggggtcttcc ggcccgtctc ggccgagttg gttgcccaca acctcaagct
    22681 cgccgcccac ggctgggccc tcaagcactg ggatgtgggc gcgcgacttg acctcacggc
    22741 atacatcgat gcggagttag acctcctgct cgccgccatc cgcgc
//
LOCUS       DAORPW010000026        22301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_384_length_22301_cov_751.149016, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000026 DAORPW010000000
VERSION     DAORPW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 22301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22301
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_22301_cov_751.149016"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3844)
                     /locus_tag="PLB86_03855"
     CDS             complement(<1..3844)
                     /locus_tag="PLB86_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HRC64301.1"
                     /translation="MTDVNPVAVVGIAAIMPMAPDADAFWANITGGRYCVTDVPPERW
                     NPALYYSPDHSARDKTYSTIGGWVREFEWDPIRWKLPIPPTVSAQMDQGQRWALSAAR
                     RALMDAGWPKWTVDSDKVAVILGNAIGGEKHYLSSMRIQLPEALRRVAESATLQTLPA
                     DMQHKIVEEARALYLDNMFEITEDTMPGELSNVIAGRIANVLNLRGPNYTTDAACASG
                     LAALNSAILGLNDHQYDAVITGGVDRNMGVDAFVKFCKIGALSATGTRPFDAGADGFV
                     MGEGAALFVLKRLADAERDGDKIYSLILGIGGSSDGKGKGITAPNPVGQKLAISRAWE
                     RAGVDPSLATAIEAHGTSTRVGDASELESVLAIFGAAGAKPGSIALGSVKSNIGHLKA
                     AAGTAGLFKMVRSLHEKVLTPSLGFVTPNENVDWDRHPCKVNTTLRPWDNAGPDGVRR
                     GGVSAFGFGGTNFHVVVEEYIPGRHKAQPKTFASAAVPQTAATYAASAPSAGVTASVA
                     AGPRKAPLRGALVLGGRDDADVLAQVQAVLAQAQAGTAPAIVAPDPALADAAVRVAVD
                     YADAKDLAGKLDKLAKGLASGNPAIFKMLRQQGVFLGRGPAPKVAFLYTGQGSQYVNM
                     LKGLNSEPIVKATFDEADKVMTPLLGRPLTSFIYVDGNDEKAVKAANKQLMQTEITQP
                     AVLATDLSLTRLLAAYGIAPDMVMGHSLGEYGALVAAGSLTLDAAMEAVSARGREMAK
                     VSMEDNGAMAAVFGPLDEIQRTVDAAEGYAVVANINSYNQAVVGGATKAVETIMEQFV
                     AKGINAIRIPVSHAFHTSIVAPASVPFVNALRRLNLVGPKLPIVANVTGEFYPADATT
                     ETMLDYTGKQIASPVQFVKGLQTLYAAGARVFVEVGPKKALHGFVEDVLGSHHDDVLA
                     LFTNFPKLADDAAFNQALCGLYAAGLGLRPAAPVAAPVAAAPVVAASAPAPVAASAAA
                     APAAAGAAGAPSDSVITELGKLFAGVLEQGMKLYGFPGGAAGAPGAGDTAYPAAAPAA
                     PEGAPTAPAQEPVVISGAGLGLPGVDPMFDDANVARILGGQQFISQLPATVRTKMARM
                     RVTRLVKDAATGSGSFQVIDNEADVVKLAGQHAPLDVVAQYGIDPGRDAALDTTTRMA
                     LGAGFDALRDAGIPLVMHYKKTTIGSQLPDRWGLPHSMRDETGIIFASAFPGYHNLID
                     EVSHYSEDRARREHLLALEGVRTHLNGSEPVCAEIDALIAQLKRELDENPFDFDRRFL
                     FRVLSMGHSQFAEIIGARG"
     gene            complement(3945..5648)
                     /locus_tag="PLB86_03860"
     CDS             complement(3945..5648)
                     /locus_tag="PLB86_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC64302.1"
                     /translation="MTDTAQTPSHALGAGLDLESLNLMLEALGDFVDAALSPELQLEL
                     DHEDRCPEDIVRSMSDPEQLGVQLVFIPEAYDGMDGGAFDAYRVCEVMARKDIGLATA
                     VFATFLGSDPIVVGATEEQKQEWLGAIAREGVVFAYGATEPEAGSDLGALETTATPVK
                     NDDGETVEYIINGRKQWISNGTIADFTTILAKTDAGPSWFVVPKDAAGFASAPPEDKH
                     GLRLSNTAALFLDDVHIPAHNLIGGVEGKGLIQAQQVFGYTRLMVAAFGLGGGWEALD
                     RAIQYSMNRVQGGSLLCEKQGYTHKLIIPHVVRLEAARAFIEDTADKIDRGLGADGAM
                     NCEGAIAKYLATVAGNAAADDAIQAHGGYGYTRPYVVEKVKRDVRITTIYEGTSEIME
                     MTIARERWQQHLKTQGGYYRTKAAELRAVHAQNSNVGADIAALANDAMAIVLEACRTG
                     RLTRNQHILFRLGELISYCEGAEIFAKRAAAAANDSAHEKSLKRFDANTFALMSRVYA
                     REAAARVALDGSRWVAGAADPGSPALAAVNGIPTAAIQTAQAGLVTDMDAVADAIYGR
                     N"
     gene            complement(5748..7940)
                     /locus_tag="PLB86_03865"
     CDS             complement(5748..7940)
                     /locus_tag="PLB86_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRC64303.1"
                     /translation="MWVRFVLGLLMTVVCLGIAGRRAYFLYRVGKTFQPLEAERRVDP
                     GSAVRAEVAEVAFQRKLLRWTVPGAAHFLVFWGFMVLLFTIVEAFIGLVKPDFQIPIV
                     GNFAWFGFFEDLFALLCVAALAVFTVLRIQQAPKNLGRRSRFFGSHIGPAWFVLFMIF
                     NVVWTLLAYRGASINAQELHEPANADKMGFLHGAFASQWFASILPKTNVEFWETFFLL
                     LSLAVLLGFTVFVTYSKHMHIFASIPNVAFARRPRALGALEPVYWQGTKVDFEDPGDD
                     DVMGVGKIEDFTWKGLLDFATCTECGRCQSQCPAWNTDKPLSPKVLIMGLRDHSLAKA
                     PYLLAAKNADGEVPEGGIPEAAAAEAARPLIGPEGDETAHVGEHGITGEGVIPFDMLW
                     SCTTCGACVEQCPVDIEHVDHIVDMRRYQVLMESAFPEEAGIMLRNLEHAGDPWGLGA
                     SARLEWAETLDFELRVYEGDKIPDDVEYLYWVGCAGALDDTAKRTARAVATLLHEAGV
                     EFMVLGEAESCTGDPARRMGHEFLFQMLAMQNVEVLNEAGAKRIVVTCAHCYNTLANE
                     YPQVGGHYEVVHHTTLLARLVADKRLTPVQRVDAAVTYHDPCYLGRHNRIFAAPREVL
                     GAIPGTRVQEMPRNREKSFCCGAGGARMWMDENLGERINQNRADEALATAPDVVAAAC
                     PFCITMLSDGVAQRQQEGTAAATVSVTDVSEVLLRSVRPDLVDSLSAS"
     gene            complement(7992..8954)
                     /locus_tag="PLB86_03870"
     CDS             complement(7992..8954)
                     /locus_tag="PLB86_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRC64304.1"
                     /translation="MSEVLVIAETTAAGVRKPTLELLTAARALGEPAAVVFGEASDAV
                     VAQLGEYGATKVYAVNAAEVGDYLSLPKAEAVVQIAGTANPVAILVTSGPEGKDVAAR
                     VAVRLNSGIVPDATVVALDGGNVVATQTVFAGRWQARSQIVRGPAVITMRPNATSAEA
                     AAASPSVETVDVAVSEAAKGAKITSREPKVSTGRPELTDAAVVVAGGRGVGSEEGFAV
                     IGQTADALGAAVGASRTVTDQHWAPHELQVGQTGKTVAPSLYLAAGISGAIQHRAGMQ
                     SSKTIVVVNKDPKAPLFQLADFGVVGDLHKVLPALIDEVGKRKG"
     gene            complement(9032..9811)
                     /locus_tag="PLB86_03875"
     CDS             complement(9032..9811)
                     /locus_tag="PLB86_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRC64305.1"
                     /translation="MKIVVLVKYVPEPTATWKFAPDLTVDRAGVEGRLSELDEYAVEQ
                     AAQLAEKGVASEVVFLTVGPAKASEGLRKALAIGGDKAVHVVDDAIHGSCALSTSLVL
                     AKALEKEGFDLVLTGMASTDAELSVVPSMVADRLGINQATFAGELSVEGGAVTIRRDS
                     DVASERITASLPAIVSVTDQTGEARYPAFKAIMGAKKKPITTWSLADLGVDAAAVGLG
                     AASVRTIDVAPTPARTAGTIITDDGEGAAQLADFLAGRGFI"
     gene            complement(9943..10890)
                     /locus_tag="PLB86_03880"
     CDS             complement(9943..10890)
                     /locus_tag="PLB86_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRC64306.1"
                     /translation="MTSVLFDHPGPKARVRHRIIAAVSAALVLLALWWALAQFKEKGN
                     LDAAKWTPFLTADLWVNYLLPGLQNTLIAAALSIVFAGAFGLVFGMGRLSHVPAIRWV
                     STVVVEFFRSVPVLVMMFFAFNLYAHNGVFRSDLNPLAGVVTGLTLYNGAVVAELLRS
                     GVGSLPKGQTEAGLAIGLSRGQTLRSVLLPQAITAMLPALVSQLVVILKDTALGYLIT
                     YQELLNKFQQIGSYKSNPVPALIVIAAIFIVINYALTSLASYLERRTRQRGRATIPKG
                     AIGPAPVDTQPIARALHDADEEVLHERYRAPGRTDDEHP"
     gene            complement(10887..11552)
                     /locus_tag="PLB86_03885"
     CDS             complement(10887..11552)
                     /locus_tag="PLB86_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRC64307.1"
                     /translation="MGELFASYNILGAFGLTILLAVFSAIGSLLIGTVVAIMRVSPID
                     FLNKVGAFYVNIVRNTPLTLIIVFCNIGFIVNLGMSFTENIARNNIIWAIIGLSFYHA
                     AFVCEALRSGVNTVPVGQAEAARSIGLTFSQSLSEVLLPQAFRGAITPLGNTLIALTK
                     NTTVATAIGVAEISGLMKEMMEFRADLIIVIFLLVAIGFVILTLPIGLLTTRLSTKLA
                     VKR"
     gene            complement(11640..12455)
                     /locus_tag="PLB86_03890"
     CDS             complement(11640..12455)
                     /locus_tag="PLB86_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRC64308.1"
                     /translation="MKLGRISVATIAVGVALGLAACGGGASTATGGTVKIGIKFDQPG
                     LGLKSGADYTGMDVDLAKYLAKELSLGTPEFVEAVSAQRETLIQTDQVKFIVGTYSIT
                     DARKEKVSFAGPYFIAGQDLLVRADDNSITGVESLNGKKLCSVKGSTSAANVQKAVPG
                     VNLQEYGTYTLCVDALKAKNVDALTTDDTILAGYAAQEANKGLMKVVGKTFSKESYGV
                     GLKKGDTAFCQKVTDAINKYVSSGEWQKAVDKNLGPAGYKAGAGNPPTPAPCS"
     gene            complement(12503..13231)
                     /locus_tag="PLB86_03895"
     CDS             complement(12503..13231)
                     /locus_tag="PLB86_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRC64309.1"
                     /translation="MVVITGVNKHFGDLHVLRDIDLTVGRGEVVVVLGPSGSGKSTLC
                     RTINRLEAYESGSITIDGKTLPDEGKALAQLRADVGMVFQSFNLFAHKTILENVTLGP
                     MKVRKKSKAEAEAKARELLARVGVTTQADKYPAQLSGGQQQRVAIARSLAMEPKVMLF
                     DEPTSALDPEMVNEVLDVMTSLARSGMTMIVVTHEMGFARRAADRVVFMADGEIVEVA
                     TPATFFTSPRSNRAKDFLGKILSH"
     gene            complement(13541..13927)
                     /locus_tag="PLB86_03900"
     CDS             complement(13541..13927)
                     /locus_tag="PLB86_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRC64310.1"
                     /translation="MEEGSTGAGETLEVVSSVSYDAQGNVMTAEQVAAQAAAAAEAKA
                     AADAAAAQAAAEAAAADAAAAAAATAAAAAAAAAPVERTYTVVKGDTLSAIGKKFGVN
                     WRDIASLNQVKNPDLIHPGQVFRIPS"
     gene            14051..15289
                     /locus_tag="PLB86_03905"
     CDS             14051..15289
                     /locus_tag="PLB86_03905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRC64311.1"
                     /translation="MAIHRRIQAAVAAAAAIAIAACTTTAPTSGDAAGPPGLGGASAV
                     SGTAAYPSAGVSAPATSGAAERPPVVPAPGPAPAPTPTAPAAPAPITIAFAGDIHFER
                     HLRPLLEGGDGLAELRPLLGAADITVVNLETSITTRGTPEPKSFTFRAPASALTTLAT
                     AGVDVAGMANNHAVDFGQEGLADTLAAKAASPIPVIGVGRTAAEAFAPAVFTVRGVSV
                     AVVAATQVPELTAAKFPATESRAGVAASIDNVRLLAAVRAARARYDVVVVFLHWGIER
                     TVCPDAAQVRTARALEQAGADIIVGGHQHRVLGSGWLGRAYVGYGLGNFVWWLKTASA
                     ADAATGVLTLSIDPAAVAARAAVPRDQWDGFAPIVVADDYAPLTISVADGVPRASAQS
                     AKRLAAWEAARGCTMLKGRP"
     gene            15286..16830
                     /locus_tag="PLB86_03910"
     CDS             15286..16830
                     /locus_tag="PLB86_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRC64312.1"
                     /translation="MSMRLAHDGDGTADRTGDPTGDRTGDRDRMIQALCSAFLAAIVD
                     LVVWVEPVDGVPTAHAANHLGRTRLHPGGRQEVLEGVDPIASQDPMAFLPYEGPTGEV
                     ANPSPAQSRANAYPYAAERLLSFFADPDRSPDLAIVHTPRHYFPDEGGHVGEHGSLDV
                     IQSRAPLVLSGVGVRPLGLLDEHARLVDVGPTMAVLAGVDPDDLRDAQGEPLDGRPLT
                     AYLTGETPRAVVGVLWDGAHCSDLLHLAEAGELPTVARLIERGLALRGGAVAEFPSIT
                     LTNHTSILTGVGPGRHGVMGNVYYDRGTGERVVPNDEKTWHRSSEWIHDSVRTIFELV
                     PGATASIDDAIDRGADYSTMGLVREDGSERGAGGMGDHLPKASTSPFLSDPRFIQRDG
                     YFRWGVSVDDMGLIQIQRLFAGGLATAPRFTWWANVVTDAGHHAAGPRSEPAREALRQ
                     ADARLGVFLDELAARGELDDVAIVLTADHGFEGSDPACTGSWRSALDALGIPYRDEGP
                     GFVYLV"
     gene            complement(16818..17489)
                     /locus_tag="PLB86_03915"
     CDS             complement(16818..17489)
                     /locus_tag="PLB86_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HRC64313.1"
                     /translation="MCEPLPGALPDTLPDALPDTLDIALVGVGGQGTILASNVLAELG
                     MAAGYDVKKAEVHGMSQRGGSVVSHVRWGPEVFSPIIPAGGADILVAFEKMEAARYAA
                     LLAPGGTAIVNDFAIEPITVITGGAHYPDDAAIEAAFADGGHRLMWIHGARVAEELGN
                     KFVANVVLLGALSTLLGLPEAAWAAVLDRRVKAAHLEINHRAFAAGRAEMSAPAPTRP
                     QPQTR"
     gene            complement(17482..19362)
                     /locus_tag="PLB86_03920"
     CDS             complement(17482..19362)
                     /locus_tag="PLB86_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRC64314.1"
                     /translation="MKRLLSGNEAIARAAWEAGAVLGAGYPGTPSTEILEAFAPYPDV
                     YAEWSPNEKVALDVAIGSAIAGRRAFATMKHVGLNVALDSFWSASMTGIEAGLVVVSA
                     DDPSLHSSQNEQDNRFLARWARVPCLDPADSQDSHDFMVAAFDLSERFDTPVLLRPTT
                     RVCHTSSPVEVGEIRLRRPVPDTYVRNLAKYDMAPANARARRPVIEHRVEALADFAET
                     FPLNRVEMRRTTIGIVTSGVAYAYAREVFPDASTLKLGMVYPLPRRLIADFAAQVDSL
                     IVLEELDPFVEDQLRLMGIRLHTPPLPPTYPHTKSIFPLMGELDPAVVRSSAVAAGLL
                     DASVASPVAASGIPYAAGSPAPASRTLSLTPVDVAPRPPVLCPGCPHRSSFYVLHKLG
                     VTVNGDIGCYTLGMAKPLDALHTAGCMGASIAVAHGGAQAGDQGRHVAVIGDSTFFHS
                     GIPALLNVLYNRSNVVTIIMDNRITGMTGHQDNPGSGHTLQRQETVEVDIERVVRALG
                     FPDDKVRTIPSYDVKALEKQLKTWLKEDGPAVLITQEECALLPTAKAAYLPLAVTDAC
                     NGCTICFRIGCPAILQSDVLDERTQRPLALIDQVMCTGCEICAQVCPRDAILFREQVA
                     HV"
     gene            19691..20176
                     /locus_tag="PLB86_03925"
     CDS             19691..20176
                     /locus_tag="PLB86_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64315.1"
                     /translation="MPYVDSTPSASSLPAGLQPLPDEDLNASSKRLSLAACNALVQKG
                     IIYHPAGWKFSCSSSRVGKSYNAWTFRRETVVKPGMTLAKTKAVLAHEEAHIWSLSVL
                     TPAQMTWFSRQLGKRSFFDGTTRTMPAEVWAASQAACAGYPQGPYKRVSCSLLRETLR
                     H"
     gene            21225..21884
                     /locus_tag="PLB86_03930"
     CDS             21225..21884
                     /locus_tag="PLB86_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64316.1"
                     /translation="MGPPAEHVFDVCDSQNAGEYLLAGSSGGFSAVVWRTTDYGRTWA
                     ELWREPEESASMDGYERFYWLTRIGNRIFMRADPGTKGTKPPMRYYDTRRQRMNKVPN
                     SAVTVGGSKFYPVILGDRVQRANQVCGDGRIAYFITDGKVFAFDGAQSSQVSSVWGVL
                     SPGDDGRIYLKDATGVYVLTGTTKTTILAQADLPGVSMTVANGRIWLGGSQSEIWSRP
                     L"
BASE COUNT         3433 a         7139 c         8078 g         3651 t
ORIGIN      
        1 ggccgcgggc gccgatgatc tcggcgaact gcgagtgccc catcgacagc acccggaaga
       61 ggaaccggcg gtcgaagtcg aacgggttct cgtcgagctc acgcttgagc tgggcgatga
      121 gggcgtcgat ctcggcgcag accggctcgc tgccattgag gtgggtccgg acgccttcga
      181 gggcgagcag gtgctcgcgg cgggcgcggt cctcgctgta gtggctgacc tcgtcgatga
      241 ggttgtggta gccggggaac gccgaggcga agatgatccc ggtctcgtcc cgcatcgagt
      301 gcggcaggcc ccaacggtcg ggcaactgcg agccgatggt cgtcttcttg tagtgcatga
      361 cgagcgggat gcccgcgtca cgcagcgcgt cgaagccggc cccgagggcc atccgcgtgg
      421 tcgtgtcgag ggcggcgtcg cggcccgggt cgatgccgta ctgcgcgacg acgtccagcg
      481 gggcgtgctg tccggcgagc ttgacgacgt cggcctcgtt gtcgatgacc tggaagctgc
      541 ccgaccccgt ggccgcgtcc ttgaccagcc gggtgacccg catccgggcc atcttggtgc
      601 ggacggtcgc cgggagctgg ctgatgaact gctggccgcc gaggatccgg gcgacgttgg
      661 cgtcgtcgaa catcgggtcg acaccgggca gtccgagccc ggcgccgctg atgacgaccg
      721 gctcctgcgc gggggcggtc ggcgcacctt ctggggcggc cggggccgcc gcagggtatg
      781 ccgtgtcgcc agcaccgggg gcgcctgcgg caccgcccgg gaaaccgtag agcttcatgc
      841 cctgctcgag gacaccggcg aagagcttgc ccaactcggt gatgaccgag tccgagggcg
      901 caccggcagc accggcagcg gctggcgcgg cggcggcaga ggcggcaacc ggggccggag
      961 ccgatgcagc gaccaccgga gccgcagcca cgggggcagc gaccggagcg gccgggcgca
     1021 gacccagccc ggcggcatac agaccgcaga gggcctggtt gaacgcggcg tcgtcggcga
     1081 gcttcgggaa gttggtgaag agggcgagca cgtcgtcgtg gtggctgccg aggacgtcct
     1141 cgacgaagcc gtgcagggcc ttcttggggc cgacctcgac gaagacgcgg gcgccggcgg
     1201 catacagggt ctgcaggccc ttgacgaact ggacaggtga ggcgatctgc ttgccggtgt
     1261 agtcgagcat cgtctcggtg gtggcgtcgg ccgggtagaa ctcgccagtg acgttggcga
     1321 cgatcggcag cttggggccg accaggttga gccgacgcag ggcgttgacg aacggcaccg
     1381 acgcgggggc gacgatcgag gtgtggaagg cgtggctcac cgggatccga atggcgttga
     1441 tgcccttcgc gacgaactgc tccatgatcg tctcgacggc cttggtggcg ccgccgacaa
     1501 ccgcctggtt gtaggagttg atgttggcga ccacggcgta gccctcggcg gcatcgacgg
     1561 tgcgctggat ctcgtccaac gggccgaaga cggcagccat cgcgccgttg tcctccatgg
     1621 acaccttggc catctcgcgg cctcgggcgc tgaccgcctc catggcggca tcgagggtga
     1681 gcgagccggc ggcgaccagc gcgccgtact caccgaggga gtggcccatg accatgtcag
     1741 gagcgatgcc gtatgccgcg agcagacggg tgagcgacag gtcagttgcg agcacggccg
     1801 gctgggtgat ctcggtctgc atgagctgct tgttggcggc cttgaccgcc ttctcgtcgt
     1861 tgccgtcgac gtagatgaac gaggtcaacg ggcggccgag cagcggggtc atgaccttgt
     1921 cagcctcgtc gaacgtcgcc ttgacgatgg gctctgagtt gaggcccttg agcatgttga
     1981 cgtactggct gccctggccg gtgtagagga aggccacctt gggggccggg ccgcggccga
     2041 ggaagacgcc ctgctggcgg agcatcttga agatcgccgg gttgccggag gcgagcccct
     2101 tggcgagctt gtcgagcttg cccgcgaggt ccttggcgtc ggcatagtcg acggccacgc
     2161 gcacggccgc atcggccagc gccgggtcgg gagcgacgat cgccggggcg gtgcccgcct
     2221 gggcctgggc cagcacggcc tggacctgcg ccagcacgtc ggcgtcgtca cggccaccaa
     2281 gcacgagagc accgcgcagc ggagccttcc tcgggccggc cgccacggag gcggtgacgc
     2341 ctgcggacgg ggcggacgcg gcatacgtcg cggctgtctg ggggacggcg gcggaggcga
     2401 aggtcttcgg ctgggccttg tgccggccgg ggatgtactc ctcgacgacg acgtggaagt
     2461 tggtgccgcc gaagccgaag gcagagacac caccgcgacg gacgccgtcg gggccggcgt
     2521 tgtcccatgg gcgcagggtc gtgttgacct tgcacgggtg ccggtcccag tcgacgttct
     2581 cgttgggggt gacgaagccg agcgaggggg tgaggacctt ctcgtgcagc gacctgacca
     2641 tcttgaacag gccagcggtg cctgcggcgg ccttgaggtg gccgatgttc gacttcaccg
     2701 agccgagggc gatcgagccg ggcttggccc cggccgcgcc gaagatcgcc aggacgctct
     2761 ccaactcgga agcgtcgccg acgcgggtcg aggtgccgtg cgcctcgatc gcggtggcca
     2821 aggacgggtc gaccccagcg cgctcccagg cccgagagat ggcgagcttc tgcccgaccg
     2881 ggttgggggc ggtgatgccc ttgcccttgc cgtcggacga gccaccgatg ccgaggatga
     2941 gggagtagat cttgtcgccg tcgcgctcgg cgtcggcgag ccgcttgagg acgaacaagg
     3001 ctgcgccctc acccatgacg aagccatcgg cgccggcgtc gaagggccgg gtgccggtgg
     3061 ccgacagggc accgatcttg cagaacttga cgaaggcgtc gacacccatg ttgcggtcga
     3121 cgccaccggt gatcaccgcg tcgtactggt ggtcgttgag gccgaggatc gcggagttga
     3181 gcgcggccag gccggacgcg caggcggcgt cggtcgtgta gttggggcca cgcaggttga
     3241 gcacgttggc gatccggccg gcgatgacgt tggacaactc accgggcatg gtgtcctcgg
     3301 tgatctcgaa catgttgtcc aggtagagcg cccgggcctc ttcgacgatc ttgtgctgca
     3361 tgtcggccgg gagggtctgc agggtcgccg actcggcaac ccgccgcagc gcctcgggca
     3421 gttggatgcg catgctcgac aggtagtgct tctcgccgcc gatggcgttg ccgaggatga
     3481 ccgccacctt gtcgctgtcg accgtccact tgggccaccc ggcatccatg agggcccggc
     3541 gggccgccga caacgcccac cgctgaccct ggtccatctg cgccgacacc gtcggcggga
     3601 tgggcaactt ccaccggatc gggtcccact cgaactcgcg gacccagccg ccgatcgtcg
     3661 agtacgtctt gtcccgggcc gagtggtcgg gggagtagta gagcgcgggg ttccagcgct
     3721 ccggcggcac gtcggtgacg cagtaccgcc ctcccgtgat gttggcccag aaggcatcgg
     3781 cgtcaggggc catcggcatg atggccgcga tgccgacgac ggcgacgggg ttgacgtcgg
     3841 tcatggtgtg gtgtccttcc ggtggtgcac aggcagtcga ggcgtgttgc gatcgggaag
     3901 cgttgcggaa gcgctagatg gagacagggg gccggcggtg gtgcttagtt gcggccgtag
     3961 atggcgtcgg ccacggcgtc catgtccgtg accagccccg cctgcgcggt ctggatcgca
     4021 gccgtgggta tgccgttcac cgcggcgagc gcgggcgacc ccgggtcggc cgcaccggcg
     4081 acccaccgcg acccgtcgag ggcgacgcga gcggcggcct cccgggcgta gacgcgggac
     4141 atgagcgcga aggtgttggc gtcgaaccgc ttgaggctct tctcgtgggc gctgtcgttg
     4201 gctgccgcgg cggcgcgctt ggcgaagatc tcggcgccct cgcagtagga aatgagctcg
     4261 cccaggcgga agaggatgtg ctggttgcgg gtcaggcgcc cggtgcggca ggcctcaaga
     4321 acgatcgcca tggcgtcgtt ggccagggcg gcgatgtcgg caccgacgtt ggagttctgg
     4381 gcgtgcacgg cgcgcaactc ggcggccttg gtgcggtagt agccgccctg ggtcttcagg
     4441 tgctgctgcc aacgctcacg ggcgatggtc atctccatga tctcggaggt gccttcgtag
     4501 atcgtcgtga tccggacgtc gcgcttgacc ttctcgacga catagggccg ggtgtagccg
     4561 taaccgccgt gggcctggat cgcgtcgtcg gctgcggcgt tgccggccac ggtcgcgagg
     4621 tacttggcga tcgcgccttc gcagttcatc gcgccgtcgg caccgaggcc acggtcgatc
     4681 ttgtcggcgg tgtcctcgat gaaggcgcgg gctgcttcca ggcgcacgac gtgggggatg
     4741 atcagcttgt gggtgtagcc ctgcttctca cacagcagcg agccgccctg cacgcggttc
     4801 atggagtact gaatggcccg gtccagggcc tcccagccgc cgccaaggcc gaaggccgcg
     4861 accatgagtc gggtgtagcc gaagacctgc tgggcctgga tgaggccctt gccctcgacg
     4921 ccaccgatga ggttgtgcgc ggggatgtgc acgtcgtcga ggaagagtgc ggcggtgttg
     4981 gacaaccgca ggccgtgctt gtcctccggc ggtgccgacg cgaagccagc ggcatccttc
     5041 ggcacgacga accacgaggg gccggcgtcg gtcttggcca ggatcgtcgt gaagtcggcg
     5101 atcgtgccgt tggagatcca ctgcttgcgg ccgttgatga tgtattcgac ggtctcgccg
     5161 tcgtcgttct tcaccggggt cgcggtggtc tccagggcgc cgaggtcgga gccagcctca
     5221 ggctcggtgg cgccgtaggc gaagacgaca ccctcgcggg cgatggcgcc gagccattcc
     5281 tgcttctgct cctcggtggc gccgacgacg atggggtcgg agccgaggaa ggtcgcgaag
     5341 accgcggtgg ccagaccgat gtccttgcgc gccatgacct cgcacacgcg gtaggcgtcg
     5401 aaggctccgc cgtccatccc gtcgtaggcc tcggggatga agaccaactg aacgcccaac
     5461 tgctcggggt cgctcatcga acggacgatg tcctcggggc aacggtcctc gtggtcgagc
     5521 tcaagctgca actcggggga gagggccgcg tcaacgaagt cgccgagcgc ctcgagcatg
     5581 aggttgaggc tctccaggtc caggcctgcg ccgagcgcgt gtgagggggt ctgggcggtg
     5641 tcggtcatgg cgagtccttg ctgggattgg gcgggtggcg ggcaggtctg gtgctattca
     5701 gttcgagagc gggcgcgggc gcacggcggg tatgccgccc acgcgggcta ggaggcggac
     5761 aacgagtcga cgagatcggg tcggaccgag cgcaacagca cctcggacac gtcggtcacc
     5821 gagacggtcg cggccgcggt gccctcctgc tggcgctgtg cgacgccgtc ggagagcatc
     5881 gtgatgcaga aggggcaggc cgccgcgacg acatcgggcg cggtggccag ggcctcgtcg
     5941 gcgcggttct ggttgatccg ctcgccgagg ttctcgtcca tccacatccg ggcgccgccg
     6001 gcgccgcagc agaacgactt ctcgcggttg cgcggcatct cctggacccg ggtgccgggg
     6061 atcgcgccga gcacctcgcg aggggcggcg aagatccggt tgtgccgacc gaggtagcac
     6121 gggtcgtggt aggtcactgc ggcgtcgacg cgctggaccg gggtcaggcg cttgtcggcg
     6181 acgagacggg ccagcaacgt cgtgtggtgg acaacctcgt agtggccgcc gacctgcggg
     6241 tactcgttgg ccagcgtgtt gtagcagtgg gcgcaggtga cgacgatccg cttggctccg
     6301 gcctcgttga ggacctcgac gttctgcatc gcgagcatct ggaagaggaa ctcgtgcccc
     6361 atccggcggg ccgggtcacc ggtgcaggac tcggcctcgc cgagcaccat gaactcgacc
     6421 ccggcctcgt gcaacagggt ggcgacggcg cgggcggtgc gcttcgcggt gtcgtcgaga
     6481 gcgccggcgc agccgaccca gtagaggtac tcgacgtcgt cggggatctt gtcgccctcg
     6541 tagacgcgca actcgaagtc gagggtctcg gcccattcca gccgggccga ggcgcccagg
     6601 ccccacgggt cgcccgcgtg ctcgaggttg cgcagcatga tgccggcctc ctcggggaag
     6661 gccgactcca tgagcacctg gtagcgacgc atgtcaacga tgtggtcgac gtgctcgatg
     6721 tcgacggggc actgctcgac gcaggcgccg caggtcgtgc aggaccacag catgtcgaag
     6781 gggatgacgc cctcgccggt gatgccgtgc tcgccgacgt gcgcggtctc gtcgccttcg
     6841 gggccgatga gcggacgggc cgcttcggcc gccgcagcct cgggtatgcc gccctcgggc
     6901 acctctccgt cggcgttctt cgccgccagc aggtaggggg ccttggcgag cgagtggtca
     6961 cgcagcccca tgatgaggac cttgggggac agcggcttgt cggtgttcca ggcggggcac
     7021 tgactctggc agcggccaca ctcggtgcac gtcgcgaagt ccagcaggcc cttccaggtg
     7081 aagtcctcga tcttgccgac gcccatgacg tcgtcgtcgc ccgggtcctc gaagtcgacc
     7141 tttgtgccct gccagtagac cggctccagt gccccgagag cccgggggcg gcgcgcaaag
     7201 gcgacgttgg ggatcgaggc gaagatgtgc atgtgcttgc tgtaggtgac gaacaccgtg
     7261 aagccgagca gcaccgcgag tgacaggagc aggaagaacg tctcccagaa ctcgacgttg
     7321 gtcttgggca ggatcgaggc gaaccactgc gaggcgaacg ccccgtgcag gaagcccatc
     7381 ttgtcggcgt tggccggctc gtggagctcc tgggcgttga tcgaggctcc gcggtaggcc
     7441 agcagggtcc acacgacgtt gaagatcatg aacaggacga accacgccgg gccgatgtgc
     7501 gagccgaaga agcggctgcg ccgaccgagg ttcttcgggg cctgctggat gcgcagcacc
     7561 gtgaacacgg ccagcgccgc cacacacagc agggcgaaga ggtcctcgaa gaacccgaac
     7621 caggcgaagt tgccgacgat cgggatctgg aagtcgggct tgaccaaccc gatgaaggct
     7681 tcgacgatcg tgaacagcag gaccatgaag ccccagaaca cgaggaagtg cgcggcgccc
     7741 ggcacggtcc agcgcaggag tttgcgctgg aaggcgacct cggcgacctc cgcccgcacc
     7801 gccgagccgg ggtcgacgcg ccgctcagcc tccagcggct ggaacgtctt gccgacgcga
     7861 tagaggaagt acgcccggcg cccggcaatc ccgaggcaga cgacggtcat gagcagaccg
     7921 aggacgaaac ggacccacat cgggaatcga gctccttcgt gccgtctgga cgggagaggt
     7981 acggcgccgg gtcagccctt gcgcttccct acctcgtcga tgagagccgg caagaccttg
     8041 tgcaggtcac cgacgacgcc gaagtcggcg agctggaaga ggggggcctt ggggtccttg
     8101 ttgaccacga cgatggtctt gctgctctgc atgccggcgc ggtgctggat ggctccggag
     8161 atgccggcgg cgaggtagag cgacggcgcc acggtcttgc cggtctggcc cacctgcaac
     8221 tcgtgcggtg cccagtgctg gtcggtgacg gtgcgcgatg ctccgacggc tgcgccgagc
     8281 gcgtccgcgg tctggccgat gacggcgaag ccctcctcgg agccgacgcc acgcccacca
     8341 gcgacgacaa cggccgcgtc ggtgagctcg gggcgaccgg tggacacctt gggctcgcgg
     8401 gaggtgatct tggcgccctt ggcggcctcg ctcacggcga cgtcgaccgt ctcgaccgac
     8461 ggggacgccg cagcggcctc ggccgacgtg gcgttgggcc gcatggtgat gacggccggg
     8521 ccgcgaacga tctggctgcg ggcctgccag cggccggcga agacggtctg ggtcgcgacg
     8581 acgttcccgc cgtcgagggc gacgaccgtg gcgtcgggca cgatgcccga gttgagccgg
     8641 accgcgaccc gagccgcgac gtccttgccc tcggggccgc tggtcacgag gatcgcgacc
     8701 gggttggcgg tgccggcgat ctggacgacg gcctcggcct tgggcagcga caggtagtcg
     8761 cccacctcgg ccgcgttcac ggcatacacc ttggtggcgc cgtattcgcc gagctgcgcg
     8821 acgacggcgt cagaggcctc gccgaagacg acggcggcgg gctcgccaag cgcacgggcg
     8881 gcggtgagca gttcgagggt gggcttgcgg acgccggccg cggtggtctc ggcgataacg
     8941 aggacttccg acacgatgag tctcctggat tggtatgccg cggcgccgcg gcggtctgtc
     9001 gggtggggtc ggcgacgtgg tcgccggaag gtcagatgaa gccgcgaccg gcgaggaagt
     9061 cggcgagctg ggcagcgccc tcaccgtcat cggtgatgat cgtgccggcg gtgcgcgcgg
     9121 gtgtcggggc gacgtcgatg gtgcgcacgc tcgcggcgcc cagaccgacg gcagccgcgt
     9181 cgaccccgag gtcggccagc gaccaggtgg tgatcggctt cttcttggca cccatgatcg
     9241 ccttgaaggc ggggtagcgg gcctcgcccg tctggtcggt caccgagacg atggccggca
     9301 gcgacgcggt gatccgctcg ctggccacgt cggagtcacg gcggatcgtc acggccccgc
     9361 cctcgacgga caactcgccg gcgaaggtgg cctggttgat gcccaggcgg tcggcgacca
     9421 tcgacgggac caccgagagc tcggcgtcgg tcgaagccat gccggtgagg acgaggtcga
     9481 agccttcctt ctccagcgcc ttggccagca ccagcgaggt gctcagcgca cacgatccgt
     9541 ggatcgcgtc gtcgacgacg tgcaccgcct tgtcgccgcc gatggccagc gccttgcgca
     9601 gaccttcgct ggccttggcg ggcccgacgg tgaggaagac gacctcggag gcgacgccct
     9661 tctcagccaa ctgggcggcc tgctcgacgg catactcgtc aagctcggac aggcgccctt
     9721 cgacgccggc gcggtcgacg gtgagatctg gggcgaactt ccaggtggcc gtgggctccg
     9781 gcacgtactt gacgaggacg acgatcttca tgcgtgatga cctgccttag ggtgcggtgc
     9841 ggcgttcggg tttgagcctg ccagagatgc tcgaatcggg aagggggtca taggtccctt
     9901 accggcgagt agcaggagtt tcgggtgccg ccgagcgtgg acctaagggt gctcgtcgtc
     9961 cgtccgcccc ggggcgcggt agcgctcgtg gaggacttct tcgtcggcgt cgtgcagggc
    10021 gcgggcgatc ggctgggtgt ccacgggggc ggggccgatc gcgcccttgg ggatggttgc
    10081 gcggccacgt tggcgggttc gccgctcgag gtaggaggcc agcgaggtga gggcgtagtt
    10141 gatgacgatg aagatggctg cgatgacgat gagggccggc accgggttgg acttgtagga
    10201 cccgatctgc tggaacttgt tgagcagttc ttggtaggtg atgaggtagc cgagggcggt
    10261 gtccttgagg atgacgacca actggctgac caacgccggc aacatcgccg tgatcgcctg
    10321 gggcaacagg acgctgcgca gggtctggcc gcggctgagg ccgatcgcga gcccggcctc
    10381 ggtctgcccc ttgggcaggc tgccgacgcc ggagcgcagc aactcggcga cgaccgctcc
    10441 gttgtagagg gtgaggccgg tgacgacgcc ggccagcggg ttgaggtcgc tgcggaagac
    10501 cccgttgtgc gcgtagaggt tgaaggcgaa gaacatcatc acgagcaccg gcaccgaccg
    10561 gaagaactcg acgaccaccg tgctcaccca gcggatcgcc ggaacgtggg acagccggcc
    10621 catcccgaag accaacccga aggctccggc gaagacgatc gagagcgcgg ccgcgatgag
    10681 ggtgttctgc aggcccggca ggaggtagtt gacccagaga tctgcggtga ggaacggggt
    10741 ccacttggcc gcgtcgaggt tgcccttctc cttgaactgc gccagcgccc accacagggc
    10801 gaggaggacc agggcggcac tgacagccgc gatgatccgg tgccggaccc gggccttggg
    10861 gccggggtgg tcgaagagga ccgaggtcat cgcttcaccg ccaacttggt cgacaggcga
    10921 gtcgtcaaca ggccgatcgg cagggtgagg atgacgaaac cgatggcgac gagaaggaag
    10981 atgacgatga tcaggtcggc gcggaactcc atcatttcct tcatcaggcc gctgatctcg
    11041 gccacgccga tggcggtggc gacggtggtg ttcttcgtca gggcgatgag ggtgttgccc
    11101 agcggcgtga tcgccccgcg gaacgcttgc gggagcagga cctcgctcag gctctgcgag
    11161 aaggtcagcc cgatcgatcg ggcggcctcg gcctggccaa ccggcacggt gttgacgccg
    11221 ctgcgcaggg cctcacagac gaaggctgcg tggtagaacg acagcccgat gatcgcccag
    11281 atgatgttgt tccgggcaat gttctcggtg aacgacatgc cgaggttgac gatgaagccg
    11341 atgttgcaga agacgatgat gagggttagc ggcgtgttgc ggacgatgtt gacgtagaac
    11401 gcgccgacct tgttgaggaa gtcgatgggg gagacccgca tgatcgcgac gacggtgccg
    11461 atgagcagcg atccgatggc ggagaacacg gcaagcagga tggtcagccc gaaagccccg
    11521 aggatgttgt agctggcgaa cagctcaccc atcgcgacgc tccttcctcg tgagtgagga
    11581 gacggggtat gccgcccgcg ctcgctgcgc gggcggcata ccggcgtctg tggatgggat
    11641 caggagcagg gggcgggggt cggcgggttg cccgcgccgg ccttgtaacc cgccgggccg
    11701 aggttcttgt cgacggcctt ctgccactca ccactggaga cgtacttgtt gatggcgtcg
    11761 gtgaccttct ggcagaaggc ggtgtcgccc ttcttgaggc ccacaccgta actctccttg
    11821 gagaaggtct tgccgacgac cttcatcagg cccttgttgg cttcctgcgc ggcatacccg
    11881 gcgaggatgg tgtcgtcggt cgtcagggcg tcgacgttct tggccttgag cgcgtccaca
    11941 cagagcgtgt aggtgccgta ctcctgcagg ttgacgccgg ggacggcctt ctggacgttg
    12001 gcggccgagg tcgacccctt gaccgagcag agcttcttgc cgttgagcga ctcgaccccg
    12061 gtgatggagt tgtcatcggc gcggaccagc aggtcctggc cggcgatgaa gtagggaccg
    12121 gcgaaggaga ccttctcctt gcgggcgtcg gtgatggagt aggtgccgac gatgaacttc
    12181 acctggtcgg tctggatcag cgtctcgcgc tgtgccgaca ccgcctcgac gaactcgggt
    12241 gtccccaggc tgagttcctt ggcgaggtac ttcgccaggt cgacgtccat gccggtgtag
    12301 tcggccccgc tcttgagccc gagtccgggc tggtcgaact tgatgccgat cttgacggtg
    12361 ccgcccgtgg cggtgcttgc gccgccgccg caggcggcaa gcccgagggc cacccccacc
    12421 gcgatcgtgg cgacactgat gcgtccgagc ttcattgcgt cctcctgaag tgatctatcg
    12481 gatcgagcag cacacggtct gctcagtggc tgaggatctt cccgaggaag tccttggccc
    12541 ggttggagcg gggagaggtg aagaaggtgg cgggggtggc gacctcgacg atctcgccgt
    12601 cggccatgaa gaccacccgg tcggccgctc ggcgcgcgaa ccccatctcg tgggtgacga
    12661 cgatcatcgt catcccggat ctggccaggc tcgtcatcac gtcgagcacc tcgttgacca
    12721 tctcggggtc cagggccgag gtgggctcgt cgaagagcat gaccttgggc tccatcgcca
    12781 ggctccgggc gatggcgacc cgctgctgct gaccgccgga cagctgggcc gggtacttgt
    12841 cggcctgggt ggtgaccccg acccgcgcga gcaactcgcg ggccttggcc tcggcctcgg
    12901 ccttgctctt cttgcggacc ttcatcgggc ccagcgtgac gttctcgagg atcgtcttgt
    12961 gcgcgaagag gttgaaagac tggaagacca tccccacatc ggcgcgcagt tgcgccaggg
    13021 ccttgccctc gtcgggaagg gtcttgccgt cgatggtgat cgacccggac tcatacgcct
    13081 cgagccggtt gatcgtccgg cagagggtgg acttgcccga ccccgagggg ccgaggacga
    13141 cgacgacctc gccccgaccg accgtgaggt cgatgtcgcg cagcacatgg aggtcgccga
    13201 agtgtttgtt gacccccgtg atgacgacca tcggctcctt ggtggacgcg gcggcgagtg
    13261 ggggttcgtc cccctctgct ggccgtccgg tgggattctg gtcccgcgct gcctcggtca
    13321 ttcgctcaac ctagcgcccc accgcgcatt tgggcagggg acgtcgtggg ctttccgaat
    13381 tgccgtcacc tgggcaacac aagcgcattc ccacagaaac aaaaaggcaa aacaccgccg
    13441 agtatgctgc gcggccggtg cggacctccg gacgcagaca gggggcctcc cggttcggga
    13501 ggccccctgc gtcgtggtgc ggagtcccca gctgactgcg tcagctgggg atgcggaaga
    13561 cctgtccggg gtggatgagg tcggggttct tgacctggtt gagcgaggcg atgtcgcgcc
    13621 agttcacgcc gaacttcttg ccgatcgccg acagggtgtc ccccttgacg acggtgtagg
    13681 tccgttcgac cggtgcagcg gcggccgcag cggcagcggc ggtagcagca gcggcggcag
    13741 ccgcgtctgc ggcggcggcc tctgcggcag cctgggctgc cgcggcatct gccgcagcct
    13801 tggcctcggc tgcggcggca gcctgggcgg cgacctgctc ggccgtcatg acgttgccct
    13861 gggcgtcata gctgaccgag gaaacgacct ccagggtctc gccggcgccg gtgctgcctt
    13921 cttccatgag tgccacgggt ggctccttct cctgttttcc tagccgggcc gagaaggtcc
    13981 ggcgccacga gtccccccgt gggtgcagtg agcctagccg ggtcggcccc gtaggacaga
    14041 ctgatctgcc atggccatcc accgtcggat ccaggccgcc gtggccgccg ccgctgcgat
    14101 cgccatcgcg gcgtgcacga cgaccgcgcc cacgtcgggt gacgcggccg gcccaccggg
    14161 cttgggaggg gcgagcgcgg tgtcggggac ggcggcatac ccgagcgcgg gagtgtcggc
    14221 accggccacg agcggcgccg ccgagcgacc gccggtggtc ccggccccgg gtccggcacc
    14281 cgcacccact ccgacggcac cggcggcgcc tgcgccgatc accatcgcgt ttgcgggtga
    14341 tattcacttc gagcgacacc tgcggccctt gctcgagggt ggggacgggc ttgccgaact
    14401 gcgcccgctg ctgggcgcgg cggacatcac cgtcgtcaac ctcgaaacct cgatcaccac
    14461 ccgcgggacg ccggagccca agtcgttcac cttccgggcg ccggcctcgg cactgacgac
    14521 gctcgcgacc gccggcgtcg acgtggcggg gatggccaac aaccacgccg tcgacttcgg
    14581 gcaggagggg ctggccgaca cgctggcggc caaggcggcc agcccgatcc cggttatcgg
    14641 cgtcgggcgc acggctgccg aagccttcgc cccggccgtc ttcaccgtcc gcggcgtgtc
    14701 cgtggcggtc gtcgcggcga cccaggtccc cgagctcacc gcagccaagt tccccgcgac
    14761 cgaatccagg gcaggggtgg cggccagcat cgacaacgtg cggctcctcg cggccgtgcg
    14821 agcggcgcgg gcccgctatg acgtggtcgt cgtcttcctg cactggggca tcgagcggac
    14881 cgtgtgcccc gacgcggccc aggtgcggac ggctcgcgcc ttggagcaag ccggggccga
    14941 catcatcgtc ggagggcacc aacaccgggt gctgggctcc ggctggctgg gtcgggccta
    15001 cgtcggctac gggctgggga acttcgtctg gtggctcaag accgcgtccg cggccgacgc
    15061 cgccaccggt gtcctcaccc tgtccatcga ccccgccgcg gtggcggcgc gcgccgcggt
    15121 gccgcgggat caatgggacg gatttgcccc catcgtcgtc gcggacgact acgctccgtt
    15181 aactatctcg gtcgcggacg gcgtcccacg ggcatccgcc cagagcgcca agcggctggc
    15241 cgcatgggag gcggctcggg gttgcacgat gctgaagggg agaccatgag catgaggctg
    15301 gcccacgacg gggacgggac cgcggatcgg accggagatc cgacggggga ccggaccggg
    15361 gaccgggacc ggatgatcca ggctctgtgt tcggccttcc tggctgcgat cgtcgacctc
    15421 gtcgtctggg tcgagccggt ggatggagtg cccaccgcgc atgcggccaa ccatctcggg
    15481 cggaccaggc tgcatcccgg tggtcgtcag gaggtcctcg agggagtgga ccccatcgcc
    15541 agccaggacc cgatggcctt cctcccctat gagggcccga ccggcgaggt ggccaatccc
    15601 agcccggccc agtcccgggc caacgcctat ccgtatgccg ctgaacggct gctgtccttc
    15661 ttcgccgatc ccgatcgcag ccccgacctg gccatcgtcc acaccccgcg gcactacttc
    15721 cccgacgagg gtgggcatgt cggagagcac gggtccctcg atgtcatcca gtctcgtgcg
    15781 ccgttggtgc tctcaggtgt cggtgtccgc ccgcttggcc tgctcgacga gcacgcccgg
    15841 ctggtcgatg ttggcccgac gatggccgtc ctcgcggggg tcgacccgga cgacctgcgc
    15901 gacgcccagg gtgagccgct cgacggacgg ccgctcacgg catacctcac gggggagact
    15961 ccgcgcgcgg tggtgggcgt cctctgggac ggggcgcact gttcggacct cctgcacctg
    16021 gccgaggccg gcgaattgcc aacggtggcg cggctcatcg agcgcgggct ggcgctgcgc
    16081 gggggagcgg tggcggagtt cccgtcgatc acgctgacga accacacctc gatcctcacg
    16141 ggtgtcgggc cgggtcggca tggggtcatg ggcaacgtct actacgaccg cgggaccggc
    16201 gagcgggtcg tgcccaacga cgagaagacc tggcaccgat cctccgaatg gatacatgac
    16261 tccgtgcgga cgatcttcga gctggtccct ggggccacgg cgtcgatcga cgacgcgatc
    16321 gaccggggcg cggactactc gacgatgggg ctcgtccgcg aggacgggtc ggagcggggc
    16381 gccggaggca tgggcgatca cctgccgaag gcgtcgacct cgccgttctt gtcagacccg
    16441 cggttcatcc agcgcgacgg ctacttccgg tggggcgtct cggtggacga catgggcttg
    16501 atccagatcc agcgcctctt cgccggcggg ctggcaactg ccccgaggtt cacctggtgg
    16561 gccaacgtcg tcaccgacgc cgggcaccat gccgcaggcc cccgctccga gccggcccgg
    16621 gaggcactgc gccaggccga cgcgcggctc ggggtgttcc tcgacgagct cgcggcccgc
    16681 ggcgagctcg acgacgtcgc gatcgtgctc accgccgacc atggcttcga aggctcagac
    16741 ccggcctgca ccgggtcgtg gcgatcggcg ttggacgcgc tggggatccc ctatcgcgac
    16801 gaggggccgg ggttcgtcta cctcgtctga ggctgcggtc gggtgggtgc cggggccgac
    16861 atctcggcgc ggccggcggc gaaggcccgg tggttgatct ccaggtgagc cgccttgacc
    16921 cggcgatcaa ggacggcggc ccaggcggcc tcgggaagcc cgagcagggt cgaaagcgca
    16981 ccgagcagga cgacgttggc gacgaacttg ttgcccaact cctcagcgac ccgagccccg
    17041 tggatccaca tcagccggtg tccgccgtcg gcgaaggccg cctcgatggc cgcgtcgtcg
    17101 gggtagtggg ccccgccggt gatgaccgtg atcggctcga tggcgaagtc gttgacgatg
    17161 gccgtgccgc cgggagccag cagcgcggca taccgggcgg cctccatctt ctcgaaagcg
    17221 acgaggatgt ccgctccgcc ggcggggatg atcggcgaga acacttccgg gccccaccgc
    17281 acgtgcgaga cgacgctgcc gccgcgctgg gacatgccgt ggacttcggc cttcttcacg
    17341 tcgtaacccg cggccatgcc caactcggcc aggacgttgc tcgcgaggat cgtcccttgc
    17401 ccgccgacgc cgacgagcgc gatatcgaga gtgtcgggga gcgcgtcggg gagagtgtcg
    17461 gggagcgcgc ccgggagcgg ctcacacatg cgccacctgc tcgcggaaga ggatcgcgtc
    17521 gcgcgggcac acctgggcgc agatctcgca cccggtgcac atcacctggt cgatgagggc
    17581 cagcgggcgt tgggtgcgct cgtcgaggac gtcggactgc aggatcgccg ggcagccgat
    17641 ccggaagcag atcgtgcagc cgttgcaggc gtcggtcacc gccagcggca agtaggccgc
    17701 cttggccgtc ggcaggaggg cgcattcctc ctgggtgatg agcaccgccg ggccgtcctc
    17761 cttgagccag gtcttgagct gcttctccag ggctttgacg tcataggacg ggatggtgcg
    17821 gaccttgtcg tcggggaagc cgagggctcg cacgacccgc tcgatgtcga cctcgacggt
    17881 ctcctgccgc tgcagagtgt ggccggagcc ggggttgtcc tgatggccgg tcatgccggt
    17941 gatccggttg tccatgatga tcgtcacgac gttgctgcgg ttgtagagca cgttgagcag
    18001 cgccgggatg ccgctgtgga agaacgtcga gtcaccgatg acggccacgt gccgcccctg
    18061 gtcgccggcc tgcgcgccgc cgtgcgcgac ggcgatgctc gcgcccatgc atcctgccgt
    18121 gtgcagcgcg tcgagcggct tggccatccc cagcgtgtag cacccgatgt cgccattgac
    18181 ggtgacgccc aacttgtgca ggacatagaa cgacgaccgg tgcgggcacc ccgggcagag
    18241 gaccggcggg cggggggcca cgtcgacggg ggtgagcgag agggtgcggg aggcgggggc
    18301 ggggctgccc gcggcatacg gtatgccgct cgcggccacg gggctggcaa cggaagcgtc
    18361 gagcaggccc gcagcgacgg cgctggagcg gacgaccgcc gggtcaagct cacccatgag
    18421 ggggaagatc gacttcgtgt gcgggtaggt cggggggagc ggcggcgtgt gcagccgaat
    18481 gcccatgagc cgcagttggt cctcgacgaa cgggtcgagc tcctcgagga cgatgagcga
    18541 gtccacttgg gccgcgaagt cggcgatgag gcggcggggg agcggataga ccatgccgag
    18601 tttgagggtg cttgcgtcgg ggaagacctc gcgggcgtag gcgtacgcga cgccgctggt
    18661 gacgatgccg atcgtcgtgc gacgcatttc cacccggttc aaggggaagg tctcggcaaa
    18721 gtcggcaaga gcctcgaccc ggtgttcaat cacggggcgg cgggctcggg cgttggccgg
    18781 tgccatgtcg tacttcgcga gattgcgtac gtacgtgtcg gggaccggtc gacgcagccg
    18841 gatttcgccg acctcaaccg gagaactggt gtgacacacc cgagtggtcg ggcgcagcag
    18901 gacgggggtg tcgaaccgct cggacaggtc gaaggcggcg accatgaagt cgtggctgtc
    18961 ctggctgtcg gcagggtcga ggcaggggac ccgggcccaa cgagccagga agcggttgtc
    19021 ctgctcgttc tgcgatgagt gcaggctggg gtcgtcggcc gagacgacca cgaggccggc
    19081 ctcgatgccg gtcatcgacg ccgaccagaa cgagtcaagt gcgacgttga ggccgacgtg
    19141 cttcatcgtg gcgaaggcgc ggcgaccggc gatcgcggac ccgatggcga cgtcgagggc
    19201 gaccttctcg ttgggtgacc actcggcata cacatcgggg tagggggcga acgcttcgag
    19261 gatctccgtg ctgggggtcc cggggtagcc cgcgccgagc accgcgcctg cctcccatgc
    19321 ggctcgcgcg atggcttcgt tacccgacag gagacgcttc atgggtgggt cctcctcgac
    19381 cggcccgcgg gatcgccggg gcgctacttc gcacgatcta acgcggtcgc ggcgtcgtcc
    19441 agtgccatag gtccctcgac tcgggggttc cccaaagtcg cgttctgacg ttgatcgagg
    19501 ttaatgtcag acgtcacaag gggtcaaaac gttgatttgg gtttcctatt cttaactaag
    19561 gtgtcagtat ggaggtatga gaagcgctct ccccgcactc aggcaggcat ccaaaagaag
    19621 cctggccctg gccatcacct tcctcacgat gggccttgtc ggactggtca tcccagcctc
    19681 agcgtcggcc atgccgtacg tcgactcgac cccctccgca tccagtctcc ccgccggtct
    19741 ccaacccctg ccggatgagg atctgaacgc ctcctccaag cgcctgtcgc tcgccgcgtg
    19801 caacgcgctg gtccagaaag gcatcatcta ccacccggcc gggtggaagt tctcctgctc
    19861 ctccagtcgg gtcggcaaga gctacaacgc ctggaccttc cggcgcgaaa cggtcgtcaa
    19921 gccaggtatg acactggcca agaccaaggc cgtccttgcg cacgaagaag cccacatctg
    19981 gtcgctgtca gtgctcacgc cagcgcagat gacgtggttc agtcgacagt tgggcaagag
    20041 gtccttcttc gacggcacga cgaggacgat gccggccgag gtctgggcag cgtcccaggc
    20101 tgcgtgtgct ggctaccctc agggcccgta caagcgagtc tcgtgctcgc tgcttcgaga
    20161 gaccctgagg cactgaggtc ctccgcttct ctgtgtgggc gtcatctccg cccacacaga
    20221 gaagcggaac tcgtggggcc gagtagatcg acgctgtcct atcgcgccga tgatatgtgg
    20281 tcgacctttc ggaaggtcgt cgactattga gcggaccagc tgccaacgag cgatagcgac
    20341 gcgcatccct ttctgtcgag tagctacccc ggtctgtcat gcccgccatg tcgctcgctg
    20401 actactggac cctcagcggg cggtgtggct cttgtgaaca aaggcccgat ggataccgcc
    20461 tcggcggatc cgttgggtcg tcaattgggg ccgccgcgtg ctgggtgatc cagcccctgg
    20521 agcagacctc tcgtcacggc caaccccccc ctcctgggtc gggacgagct gtctccaggc
    20581 gggcccgaca tcgtcgcatc cgcagatgtg accccccttg ctttacccgc gcacttttga
    20641 acacttttga gaggttcgtc atgaggagca ccagtcctcg tgttgcagat cgccacggct
    20701 tggtcgcggc aacaccgtat cggcccgctc acatggaaga tgtgaacgct tttccgctcc
    20761 gcaggtcatc cgtcggcgtg tctcgccgca cgcttctcgt cggcgtcgct gggggcctcc
    20821 ttgccacagc gcttgacatc gacgctgtcg ccaaccccct actcgaccct acgggaaccc
    20881 cgccggcggg cttcaccttc cacggccgag ccccagatgc gctcgcacac acggcctaca
    20941 gcggagggtg catacacacc ttgatcgggt accagggtcg catctactac ggctacggga
    21001 actacggtgc aaactccggg tcggcgtccg gaatgggcac taacgtttcc tacttcggtt
    21061 cggcgggagg tttcggttca tcattcacct cgttccgaac cgaggagatt aacacgtaca
    21121 ggcggtttgg tggtgcgcta ttcgccccat gtatcgatcc ctcgtctgga gctcccgcag
    21181 gcaatagctt tgcctcgaat ctagacggca gatgggccgc ctttgtgggt ccgccggccg
    21241 agcacgtttt tgacgtgtgc gactcccaga acgcggggga gtatctgctt gccggctcgt
    21301 ccggcggctt ctccgcagtg gtgtggcgca cgaccgatta tggccggacg tgggctgaac
    21361 tgtggcgtga gccggaggag tccgcgtcca tggacgggta cgagcgtttc tattggctga
    21421 cgcgtattgg taaccgcatc tttatgcgcg ctgatcccgg gacaaagggt accaagcccc
    21481 cgatgcgata ctacgacacg agacgtcagc gcatgaacaa ggtaccaaac agtgccgtga
    21541 cggtcggtgg atcgaaattc tacccggtta tccttggaga ccgcgtgcaa agggcgaatc
    21601 aggtttgtgg cgatggacga atcgcatatt tcatcactga tggcaaagta tttgcctttg
    21661 atggcgctca atccagtcag gtatcttcag tgtggggcgt tctgtcgccc ggcgatgacg
    21721 gacggattta cctcaaggac gccactggcg tctacgtctt gacgggaacc acaaagacca
    21781 cgatcttggc tcaggccgac cttcccggtg tgagcatgac agttgcgaat ggccgtatct
    21841 ggctaggcgg tagccaatcc gagatctggt ctcgtccgct ttgaattctc cccagcctcg
    21901 cgcccaatcc gctcagcccc aggtggccag ccacacgagc tcgctgggcc gccaagcgcg
    21961 gacggctgcc aacgctcaac cgaagggacc tgaccagcca tgctcacacc ccagcccggc
    22021 accccgtttc cgcccgcgca gcggccgaag gaattcttcc aagaagggac ccagcggact
    22081 agccgcgagt gcggaccgat aaatgaggcc acccatcacc tcatcaccgc gagactgcgc
    22141 accgaattct ccgccgctcg cataggggtt gctgctctcg gttgagggcg ccgatccgat
    22201 ggtgccgtgc aggcgtcggt tggtgaaccg gtcgacccag ccggcggcgc ggtcatcttc
    22261 cgcccgcacg ggcgggccct ccgcggtcag ctgacgccta c
//
LOCUS       DAORPW010000027        21999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_394_length_21999_cov_798.187842, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000027 DAORPW010000000
VERSION     DAORPW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 21999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21999
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_394_length_21999_cov_798.187842"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..660
                     /gene="rbfA"
                     /locus_tag="PLB86_03935"
     CDS             145..660
                     /gene="rbfA"
                     /locus_tag="PLB86_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRC64317.1"
                     /translation="MADAARARKIAERIKAIVAEQLEYRVKDERLGFVTVTDVRVTGD
                     LQHATVFYTVFGSDEERANTAKALESAKGKIRSALGQLGIRLTPSIDFVADALPEGAA
                     HLEDLLVATKARDEEIAKAAAGAVYAGEADPYRKPGESDDDESEDDADDSEDDPEGSG
                     DDADEPASERE"
     gene            657..1568
                     /gene="truB"
                     /locus_tag="PLB86_03940"
     CDS             657..1568
                     /gene="truB"
                     /locus_tag="PLB86_03940"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRC64318.1"
                     /translation="MSAPEGVLIVDKPTGWTSHDVVARVRRLAGTRRVGHAGTLDPMA
                     TGVLVLGIGRGTKLLTFLVGCDKGYAATIRLGAATVTDDAEGEVVSTTSAAHLTREAI
                     EAAVAPFRGAISQVPSAVSAIKVDGRRAYARVRAGEDVQLAARSVTVSRFDVLDVRPG
                     TTVDVDIEVDVSSGTFVRALARDLGTALGVGGHLVALRRTRVGCFTIGEAHDLEALSA
                     SSGEGGTGADGIPLLSLAAAGRRALPVRDLSEAEARALGFGQPVASSGPAEEAVAAFT
                     PDGHLLAVLDQTKPRARTRVVFPIEDS"
     gene            1579..2535
                     /locus_tag="PLB86_03945"
     CDS             1579..2535
                     /locus_tag="PLB86_03945"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRC64319.1"
                     /translation="MQVWNGLDEVPSGIGPCVVTLGNFDGVHRGHRAVLGVVVDAATS
                     RGLTSVAVTFHPHPIAVLFPDRAPAELTSLAQRLDLLSATGLDAVLVLEFTREFAGQT
                     PAEFVDSVFVDALNAQLVVVGSDTRFGVRNSGTVQTLIELGADRGFEVAPIADVGDEG
                     RWSSTRVRALVADGDVVAAGELLGRSPRVDGVVVHGDHRGRQLGFPTANLGDPIEGLV
                     PADGVYAGWLTRLGRPLGASDRVLPAAISIGTNPTFDGTSRRVEAYVLDRTDLDLYGE
                     RVAYEFVRMLRPTLRFDGIEPLVVQMHADVQECRETLGVPAP"
     gene            2600..3106
                     /locus_tag="PLB86_03950"
     CDS             2600..3106
                     /locus_tag="PLB86_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HRC64320.1"
                     /translation="MAGPSLPVLRLPTWQELLGRPESLRRMMNVWPPYRFAGIRVLHI
                     EPDYSRALVSLKLTPWNRNYVGTQFGGSLSSMADPFWMLLVMNQLGRDYIVWDQRAEI
                     EFVRPGTGEVRTEFVVDPAVVERIRQAAAGGQKVLEWFENDIVDMSGVLVARVRRQLY
                     VRRKRDTV"
     gene            3250..5106
                     /locus_tag="PLB86_03955"
     CDS             3250..5106
                     /locus_tag="PLB86_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRC64321.1"
                     /translation="MKSAVDERVNHDILEGRARSIAALFRDRVSRSGNREAFQYFHNG
                     HLQSVTWSAARDRVYDMAAGLMSLGLTIEDRVAIASTTRYEWALADLGVMCSGGATTT
                     VYPTTMAEDVAYILGDSGSRFVFAEDDGQVEKLRTHRHDMPNVVKVIVFNGEAHSGDE
                     EGDDWVISLADLEALGRDAREARPTMVDERIDLIAPDHLAVIIYTSGTTGRPKGAELT
                     HDAIVYEAAAVHAIGILDQNDLQFLWLPLSHVFGKILLALPLQMGFPTAIDGKIDKIV
                     DNLAVIKPTFMGAAPRIFEKAYARIAATVAAETGVKRQLMDWALDVGKQVMELRAQGQ
                     APGALLGIQHAVADKLVLSKIRERFGGRVRFFISGSAALNPEIARWFGSVGLIILEGY
                     GLTETSAATCVNRPSLGGYNYGTVGWAIPTTDVKLAADGEILVKGPGVMRGYRNRPDA
                     TAEVFDEDGYFRTGDIGEIDANGFVKITDRKKDLFKTSGGKYIAPSLIEAAFKAQCPY
                     ASQILVFGDGRNYATALITLDPDAITAWGAANRMAGKSYAEISQTPEVRELLQGYIDT
                     LNQGLNRWETIKRFTVLDRDLTIDEGELTPSLKLKRKHVAEKFKANLEALYD"
     gene            5211..7202
                     /locus_tag="PLB86_03960"
     CDS             5211..7202
                     /locus_tag="PLB86_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HRC64322.1"
                     /translation="MPGQSRFADLEPLLDRVQKPIQYVGGELNSQVKDWQCGGHGPGG
                     EELTVRWALMYPDAYEVGLPNQGVMILYEVLNERPHVLAERTYAVWPDLEALLRESGV
                     PQLTVDGHRAVVDFDLFGVSFSTELGYTNFLTAIDLAGIPLHSKDRGDGTPIVIAGGH
                     ASFNPEPIADFVDAVVVGDGEQAVLEITDLVAAWKAQGSPGGRDELLMRIARSGVAYV
                     PSCYEVDYFPDGRIRRVAPRTGLTGIPWRVSKHTVTDLDAWPYPKAPLVPLAETVHER
                     MSVEIFRGCTRGCRFCQAGMITRPVRERSITGIGEMVERGLAATGFEEVGLLSLSSAD
                     HSEIAEVTKGLADRYEGTQTGLSLPSTRVDAFNIDLANELSRGGRRSGLTFAPEGGSE
                     RLRKVINKQVSEQDLVNTVAAAFASGWRQVKLYFMCGLPTETDEDVLQIASLAKQVIE
                     TGRQVSGRRDVRCTVSIGGFVPKPHTPFQWAAQLGAEATDARLQLLRDTIRADRRYAA
                     AIGFRYHDGQPGIVEGLLSRGDRRVGAVIEAVWRDGGRFDGWSEHFSYERWMRAAETA
                     FAESPVSVDWFTTRERDRIEVLPWDHIDSGLDRDWLWDDWQDALGEHEVADCRWSGCF
                     DCGVCPEMGTQIEMGPTGRTLLPLAVLSPDRARLLETSP"
     gene            7199..7675
                     /locus_tag="PLB86_03965"
     CDS             7199..7675
                     /locus_tag="PLB86_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC64323.1"
                     /translation="MSWAGDTQGYAVRPAGPDDLAAVTDCRRRWVAERTDASDPGFED
                     RMADWWVREADRRATWLAWAGEQPIGMAATVIFERMPAPGAPTARWGYVSQVWVDAAY
                     RRRGVASTLMGAVIDWARREGLVRLVLNPSDESRPMYAALGFREASDLLRLDLSGR"
     gene            complement(7676..8050)
                     /locus_tag="PLB86_03970"
     CDS             complement(7676..8050)
                     /locus_tag="PLB86_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRC64324.1"
                     /translation="MTSQPAAPTPPPSTPSAADLAARIRATTFPRIRYHKGYDRYDVD
                     WLLRKVATELERGVAKPQLADELAKAEFSTTTVRSGYDERTVDDFVDEVVGALRALLG
                     PVPPQRTKSTPLALVRRLLKRP"
     gene            complement(8064..8597)
                     /locus_tag="PLB86_03975"
     CDS             complement(8064..8597)
                     /locus_tag="PLB86_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase family protein"
                     /protein_id="HRC64325.1"
                     /translation="MQEIETRSGWLTRDQLRDARERLPILYVNLVPVRVDERSQVTQI
                     GLLLRASETGEIQRELVSGRVLYHERLRDAVLRHIERDLGPMALPQVPIAPQPFAVAE
                     YFPTPGVTPFHDPRQHAVALAYVVVLMGDCQPQGNALDLAWLTPAEASELATTPEMVG
                     GHGVLLRQALAHLGHPI"
     gene            complement(8646..10007)
                     /locus_tag="PLB86_03980"
     CDS             complement(8646..10007)
                     /locus_tag="PLB86_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRC64326.1"
                     /translation="MPASLRRLDPLLGDAVLRDPDIAATYAVDSSYGASAPETFTVVR
                     ARDTQDVVAVLEEAQAHGIPVVPQGARTSLTGASCATEGAIVLNVEALRGIDIDPVEG
                     HARVGPGVVNADLKAAVREAGLFYPPDPASADTCTIGGNVATNAGGLCCIKYGVTADY
                     VKSLEIVLPGGEQIRTGKRTAKSTAGYDLTGLFVGSEGTLGVVTSALVRLIPAPDLAL
                     TALATFDSLDEAARAVLALRADRHRPALLEFLDRASIAAIQAMNDFGFPSGCEAALLV
                     QSDRPGHAAEDVERYAATLADLGAAEIAVADNAAEAELLMKGRRMLNYALEAKGGRLA
                     EDACVPVARLGDFVRIGQEISERTCVEITLSGHGGDGNLHPSLFFEHGDVDGWHRAEA
                     ALEELLRAALDLGGTITGEHGVGVQKRRFLEWELGAAEVARQRAVKALFDPSGIMNPG
                     KVY"
     gene            10097..11332
                     /locus_tag="PLB86_03985"
     CDS             10097..11332
                     /locus_tag="PLB86_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRC64327.1"
                     /translation="MSASYGELIAALESLPANPRIVASGNGAIPFELLHIVDHTLPAF
                     TLNMLAAHAGLPLREGVTHETSFVGAGMRKSPHLSYVPARLSMVPLLFSRTLPPDAVI
                     AHTSLPHNGKISLGIETNVVVGAIEACKARGGKIIAQANKQMPYTFGDSEYDVADFDI
                     IYEVDLPVANYQGRKTVSADSSDTAIIGALAADRIKDGSTLQLGIGAIPDAVLPELVH
                     KKRLGIWTEMFSDGVLSLLDAGAMDMDRPIVASFSVGSAELYEWMHLNEQVVMRRTET
                     TNNPANIALQPRMTSVNTALQVDLFGQANASRIKARIHSGFGGQTDFIVGALHSPGGQ
                     AIMALRSWHPKADVSTIVPLIEEPVTSFQHSAIITEQGVAEIFGHDQAHQCWSIINKA
                     AHPSVRDELWEEAHALGLA"
     gene            11350..11697
                     /locus_tag="PLB86_03990"
     CDS             11350..11697
                     /locus_tag="PLB86_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64328.1"
                     /translation="MSGVSGRAPTRDEWRVFGWWALVGLGSIWATWTLLSYGWIIAVI
                     TCAGALMLANREEPGRSAWGFVTGMGLVPLWIGWTNRAATTAGLSPSVWIGSGAVTAL
                     VGVLIFVRPRRTT"
     gene            11707..12900
                     /gene="coaE"
                     /locus_tag="PLB86_03995"
     CDS             11707..12900
                     /gene="coaE"
                     /locus_tag="PLB86_03995"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRC64329.1"
                     /translation="MLRVGLTGGIGSGKSTVAALLREWGADVTDADRVAREIVEPGMP
                     PLAAIRERFGPNVIQADGTLDRAGLAAVVFPDREALRALEAITGPAIADRVAQLRGSV
                     PVDRIDVYDMPLLVEKGLWVHEHVTIVVGADAEVRVARLVGQRGLSESDARARIAAQA
                     SDTQRHAAADAWIDNGGSREDTQAQVRRLWDARLVPFDANLRGGIRARRSDTTTLAEP
                     DPGWADAGARVVARIAAALPGQGIRVDHIGSTSVPGLVAKDVVDVQVGVPQLADADAS
                     GFAAAVRQAGYLLVAGNDQDHPHPSDAAPAHWQKRFYGGCDPGRIVHLHVREIGSAGW
                     EFALAFRDWLRAEPSAREEYAALKRELAARHSVTRDYSAAKEPWFDAAYLRVQEWARE
                     VRWTP"
     gene            complement(12904..13590)
                     /locus_tag="PLB86_04000"
     CDS             complement(12904..13590)
                     /locus_tag="PLB86_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC64330.1"
                     /translation="MSIVFLHGTGSAGPKAWPLQAAALGDEVHFVRRVHDGDHPDHVQ
                     PVVIDALAPRGHLVAHSYGCVSAVLAATERPDVVASLVLCEPPFVGLTAGWPETDAHV
                     AALAPLFERRSDPTFTDLELSAGFATAVGMPVPDFTPDQLARSAARLRAFTPAWLIAI
                     DPTIVGRVPTLVVTSGTRDIYDEVAHLLERHGATRLVLPGHGHRVQDHPDATAAFRRF
                     WGSTASAEAQ"
     gene            13679..>15129
                     /gene="uvrB"
                     /locus_tag="PLB86_04005"
     CDS             13679..>15129
                     /gene="uvrB"
                     /locus_tag="PLB86_04005"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRC64331.1"
                     /translation="MRPTTDLQRRVAPFEVISDFEPSGDQPDAIAELARRIRAGVTDT
                     VLLGATGTGKSATTAWLIEQVQRPTLVMAPNKTLAAQLANEFRELLPNNAVEYFVSYY
                     DYYQPEAYIPQTDTYIEKDSSVNDEVERLRHSATNSLLTRRDVIVVASVSCIYGLGTP
                     QEYIDRMVALKVGQEIERDQLLRRFVGMQYTRNDLAFTRGTFRVRGDTVEIIPMYEEL
                     AVRVEFFGDEIERIYTLHPLTGEVVREEQEMYVFPASHYVAGPERMERAIGGIESELE
                     TRLAELDRQGKLLEAQRLRMRTGYDIEMMRTLGFCNGIENYSRHIDGREAGSAPNCLL
                     DYFPEDFLLVIDESHQTVPQIGAMYEGDMSRKRMLVDHGFRLPSAMDNRPLKWEEFLE
                     RIGQTVYLSATPGDYEMAKSRSFGPPVEQIIRPTGLIDPEIILKPTKGQIDDLIHEIR
                     TRAARNERVLVTTLTKKMAEDLTDYLLDKGIRVR"
     assembly_gap    15130..15229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15230..15514
                     /locus_tag="PLB86_04010"
     CDS             <15230..15514
                     /locus_tag="PLB86_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRC64332.1"
                     /translation="ELLGSGRSRSRGKASGMPAGRDRAGRGAILADVGVAGGKRVEGL
                     PTSDLAGLIQELTAQMHTAAAELHFELAARLRDEVSELKKELRQMTEALR"
     gene            15590..15913
                     /locus_tag="PLB86_04015"
     CDS             15590..15913
                     /locus_tag="PLB86_04015"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HRC64333.1"
                     /translation="MTTSEVSLAAWPIGAPGVLASAAAPAGPPSVAAPTDLLTHLARL
                     GLRLGMTVTPLMRTPGRGLVLAVGDLRLAVDRASADALRVHAPGHSSPRSTSNTPDPT
                     PTVEV"
     gene            15915..17912
                     /gene="feoB"
                     /locus_tag="PLB86_04020"
     CDS             15915..17912
                     /gene="feoB"
                     /locus_tag="PLB86_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRC64334.1"
                     /translation="MGNAGLETGGAVTAAPTPSCHAPAAPQAPTGSLVVALAGAPNVG
                     KSTLFNALTGARLTMGNWPGTTVEVSRGRWQPDAGSVTYELVDLPGAYSLDPISPDEE
                     LTRAVLLDPDPAARPHVVIVAADATHLARSLYVVAQLREHAQRIIVALTMVDVAARRG
                     ITVDPGGLERALGIPVVAVDPRRRTGLTALAERVTQIAGAPEPAPRLQDTETRGERDG
                     DADELDRAEERFGWVQDAVDRAQTREAAARHTWTDRVDAVVLSRVWGPIVFLAVMWLV
                     FQATTTVAAPIQDALDALFTGPVSEGARAALAALHVDHPLVTGLVVDGLIAGVGMLLT
                     FVPLMAVMFVLLALLEDSGYLARAAVVTDRTMRALGLPGRAFLPLIVGFGCNVPAIAA
                     TRVLGGAKQRVLTALLVPFTSCSARLTVYVLLATTFFDRYAGTVVFGMYLLSILLIVG
                     VGLVLRRTLWRSMLAEPLLLDLPPYQRPTARVTWSVTWTRLRGFLRTAGGIIVATVAV
                     VWLLQSVPVRGEHAFGATPVGDSAYAALAGAVAPVFEPAGFGQWQSAGALVTGFVAKE
                     AVISSWAQTYATLDDTGAADEGRLAQAVRQDFEISSGGHGNAAALAFMVFLLAYTPCV
                     ATLAAQKREIGVRWAAFGVGMQLAVAWVLSVVVFQIARLVG"
     gene            17909..18229
                     /locus_tag="PLB86_04025"
     CDS             17909..18229
                     /locus_tag="PLB86_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HRC64335.1"
                     /translation="MTLPSQARAKPLHLVLAAIGSGVGSRGELTRHTGLAPDVVDAAV
                     DHLVATGRLTNTALGGGCATGGCGACPSGRADAGPGCGVADPRDARGPVVLTLATLAT
                     RSRG"
     gene            complement(18241..19278)
                     /locus_tag="PLB86_04030"
     CDS             complement(18241..19278)
                     /locus_tag="PLB86_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRC64336.1"
                     /translation="MDVTPLVWWVTIGAATVALLLDVAVVARRPHVPSMRECTTYLVV
                     YVSLALLFGLFIWWDFGGQYAGEFYAGWLTEYSLSVDNLFVFLLIMANFRVPERLQQS
                     ALMVGIIIAIVLRGLFIALGAAVINSYAWVFYIFGAFLIWTAWKLAREGESDDDEYEE
                     NAFLQFVERRFPATSSFHGTKLFVHEGAKRLVTPMFIVIMALGTTDLLFALDSIPAIY
                     GLTKEPYLVLTANIFALMGLRQLYFLIGGLLKRLVYLSIGLAVLLAFIGVKLILHALH
                     ENNLPFINGGQPLHGIPDIPISVSLGAIVLILGVTTIASLLKSSRDRKAAELTAGQAI
                     SDPTEDDHPDW"
     gene            complement(19312..19656)
                     /locus_tag="PLB86_04035"
     CDS             complement(19312..19656)
                     /locus_tag="PLB86_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRC64337.1"
                     /translation="MTREELLAYCLAKPGAWPDEPWDGDVVAKVGPKIFAFTGEDAVG
                     LKLGRGRDEADEWLHRYPQDASVMAYIGRYGWNTLRLAGAIPDDELLEAIDASYESVV
                     TALPRKQRPNAG"
     gene            complement(19672..20433)
                     /locus_tag="PLB86_04040"
     CDS             complement(19672..20433)
                     /locus_tag="PLB86_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRC64338.1"
                     /translation="MALLRVSVPVTTLWTSPDAPRPVDAAAIADQADPARWLAVLDAR
                     PQDDESGDGRLGLHGRVESQAVLGEPVLPISDVSADGWVRVVCPWQPSRLDPRGYPGW
                     VRRSHLEDAGSAGDEPGLPVADAPGADVLTLAAEHLGLPYLWGGTSPLGLDCSGLVHH
                     VWRRLGVVVPRDADDQEAAAYPVTFGEERPGDLYFFAHPGRAVHHVGIVSAPGRMLHA
                     PETGRGIVEEALGPDREATRYAVGRLPLPGTTQRS"
     gene            20467..21249
                     /locus_tag="PLB86_04045"
     CDS             20467..21249
                     /locus_tag="PLB86_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HRC64339.1"
                     /translation="MDPAAVTARLADLVAAAPGRLSVAVRARDGYRFDHDSARVVPAA
                     STIKVPVLLAYLASGMPWDLPVALPAARVGGCGPLALLPSVTSLPAGEALRLMIALSD
                     NDATNAVLGLVGVGEVARLLGRAGTRYTVLRRRMMDQVAAAAGHENETCAADLAELLA
                     AVRAGSILPQREAAMALAVLREQQFDEGLPAYLPEGLRCASKTGSLPGVRHDIAVIES
                     GDAWVAVAALATDLMDGPVDRGTSIYPTYAAIGETVAALVTG"
     gene            complement(21281..>21999)
                     /locus_tag="PLB86_04050"
     CDS             complement(21281..>21999)
                     /locus_tag="PLB86_04050"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRC64340.1"
                     /translation="THIEPVRYGRGSNVMSLLTSVLTDDLGDTPRWRVWLRQSWHERS
                     TVRRLYDFKHWSEHTVIALVMQSLDNSINTFPKRNRVTGKITLSSEQGHGAPNPTWIP
                     LANEAVRRMARNVGGFPAGNIGEQFHRPLTAHFIGGCPIGKDADHGVVDAYHRVFGYD
                     GLHIVDGSAISANLGVNPSLTITAQAERAMAYWPNKGQTDARPALGTAYQGIAPVEPA
                     KPIVPASAPAALRLPIVGIT"
BASE COUNT         3364 a         7505 c         7610 g         3420 t
ORIGIN      
        1 ctcgttcgcc ccacgcttgg tcgggtttca agcatcggtg tggacttcgc aaaccccacc
       61 gctgcggtat gccgcgtccg cccccaacac gggcgacgcg gcataccgca gcaaccgaag
      121 tccgttgaca gagaaggacc ttcaatggcc gacgccgcgc gggcgcgcaa gatcgctgag
      181 cggatcaaag cgatcgtcgc cgagcagttg gaataccggg tcaaggacga gcgcctcggc
      241 ttcgtcaccg tgaccgacgt ccgcgtcacc ggcgacctgc aacacgccac ggtcttctac
      301 acggtgttcg ggtcggacga ggaacgggcc aacacggcca aggcgctgga gtctgccaag
      361 ggcaagatcc gctcggcctt gggccaattg gggatccggc tcaccccgag catcgacttc
      421 gtcgccgacg ccctccccga gggggcggct cacctggaag acctcctggt cgccaccaag
      481 gcgcgcgacg aggagatcgc caaggcggcg gccggcgcgg tctacgccgg ggaggccgac
      541 ccctaccgca agccgggtga gtcggacgac gacgagtccg aggacgacgc agacgattcc
      601 gaggacgacc ctgagggctc cggcgacgac gcggacgagc ccgcgagcga acgggagtga
      661 gcgcccccga aggggtgctc atcgtcgaca aacccaccgg ctggaccagc cacgacgtcg
      721 tggctcgggt ccgtcgactg gcgggaaccc gccgcgtcgg gcacgcgggc acgctcgacc
      781 cgatggccac cggagtcctg gtgctcggca tcggccgcgg gaccaaactg ctcaccttcc
      841 tcgtcggctg cgacaagggg tatgccgcga cgatccggct aggtgccgcg acggtcaccg
      901 acgatgccga gggcgaggtg gtgtcgacga cgtcagccgc acacctcacc agggaagcga
      961 tcgaggcggc cgtggccccg ttcaggggag cgatcagcca ggtgccgtcc gcggtgagcg
     1021 cgatcaaggt cgacggacgg cgggcctacg cccgcgtgcg agccggcgag gacgtccagt
     1081 tggcggcccg atcggtgacc gtcagccggt tcgacgtgct cgacgttcgc cccgggacga
     1141 cggtcgacgt ggacatcgag gtggacgtgt catccggcac ctttgtccgg gcccttgcca
     1201 gagacctggg aacggcgctc ggcgtcggcg ggcacctcgt ggccctgcgt cgcacccggg
     1261 tggggtgctt caccatcggt gaggctcacg acctggaggc gttgtccgcc agttcagggg
     1321 agggcgggac tggggcggac ggcatacctc tgctgtcctt ggccgccgct ggccgccgcg
     1381 ccctgccggt tcgggacctg tccgaggcgg aggcgcgagc cctcggtttc ggccaaccgg
     1441 tggcgtcgag cgggcccgcg gaggaggccg ttgcggcgtt cactcccgac ggtcacctgc
     1501 ttgccgtcct tgatcagacc aagcctcggg cgcggacgcg cgtcgtgttc cccatcgagg
     1561 actcctagag tgtcctccgt gcaggtctgg aacggtctcg acgaggtgcc ttcgggcatc
     1621 ggcccgtgcg ttgtcaccct cggcaatttc gacggcgtgc accggggcca tcgcgcggtc
     1681 cttggcgttg tcgtcgacgc tgcgacctcc cgaggcctga cctcggtggc ggtcaccttc
     1741 cacccgcacc cgatcgcggt gctcttcccc gatcgcgcac ccgccgagct cacctcgctc
     1801 gcccaacggc tggacctgct gtccgccacg gggctggacg cggtgctcgt gctggagttc
     1861 acccgcgaat tcgctggcca gacgccggcg gagttcgtcg actcggtgtt cgtcgacgcc
     1921 ttgaacgcgc aactcgtcgt cgtcggatcc gacacccgct tcggggtgcg caacagcgga
     1981 accgtccaga ccctcatcga gttgggtgcc gaccgaggtt tcgaggtcgc cccgatcgcc
     2041 gatgtcggtg acgagggccg ctggtcctcg acccgcgtcc gggctctggt cgccgacggc
     2101 gacgtcgtcg cagcaggcga gctgcttggc cgctccccgc gggtcgacgg ggttgtcgtc
     2161 cacggggacc accgcgggcg ccaactcggc ttcccgacag ccaatctcgg cgaccccatc
     2221 gagggcctcg tcccagcaga cggggtgtat gccgggtggc tgacccgcct cggccgtccc
     2281 ctcggtgcct ccgaccgggt gctgcccgcg gcaatctcga tcggcacgaa cccgaccttc
     2341 gacgggacct cccgccgggt cgaggcgtac gtcctggacc ggactgacct cgacctttac
     2401 ggcgagcggg tcgcctacga gttcgtccgg atgctgcgcc ccacgctgcg gttcgacggc
     2461 atcgagccgc tggtcgtgca gatgcacgcc gatgtccagg agtgtcgcga gacgttgggc
     2521 gttcccgccc cctgaggtgc cgcccgctgg ggttccgccc gctgaggtgc cggcagccgg
     2581 cccatggcag gctgggggga tggctggtcc ttccttaccc gtccttcggc tgcccacctg
     2641 gcaggagttg ctcggtcgcc ccgagagcct gcggcgcatg atgaacgtct ggccgcccta
     2701 tcggttcgcc ggcatccggg tcctgcacat cgagcccgac tactcgcggg ccctcgtcag
     2761 cctgaagttg acgccctgga accgcaacta cgtggggacc cagttcggcg ggtcgttgtc
     2821 ctcgatggcc gatccgttct ggatgctgct ggtgatgaac cagttgggcc gtgactacat
     2881 cgtctgggac cagcgggccg agatcgagtt cgtccgcccg ggcacgggcg aggtccgcac
     2941 cgagttcgtg gtggatcccg ctgtggtgga acggatccga caggccgcgg ccggcggcca
     3001 aaaggtgctc gaatggttcg agaacgacat tgtcgacatg tcgggggtcc tcgtggccag
     3061 ggtgcgccgc caactgtatg tccggcgcaa gcgcgacacg gtgtgagtta gaccctgggg
     3121 ctcccgcctg gagccgggac agcatacggt tgggggtagc acgagcggtc gagcaacggc
     3181 gatctactga ctagtaggca ccgtccgccg acgacgagag taacgacaaa ggagtcaaag
     3241 cccgtggcga tgaagtcagc cgtggacgag cgcgtcaacc acgacatcct ggaaggacga
     3301 gcccgcagca tcgccgctct cttccgtgac cgggtgtccc gctcggggaa ccgcgaagca
     3361 ttccagtact tccacaacgg ccatctccag agcgtcacgt ggagcgccgc ccgcgaccgc
     3421 gtttacgaca tggctgctgg cctgatgtcg ctcggcctaa ccatcgagga ccgggtggcg
     3481 atcgcctcga ccacgcgcta cgagtgggcc ctggccgacc tcggcgtcat gtgttctggc
     3541 ggtgcgacga cgacggtcta ccccacaacg atggccgagg atgtcgccta catcctgggt
     3601 gactccgggt cgcggttcgt gttcgccgag gacgacggcc aggtcgagaa gctgcggacg
     3661 caccgtcacg acatgcccaa cgtcgtcaag gtcatcgtct tcaacggtga ggcgcacagt
     3721 ggcgacgaag agggcgacga ctgggtgatc tcgttggccg acctcgaggc gctgggccgc
     3781 gatgcccgtg aggcccgccc gacgatggtc gacgagcgga tcgatctcat cgcgcccgac
     3841 cacctggccg tcatcatcta cacctcggga acgaccgggc gccccaaggg cgccgagctg
     3901 acccacgacg cgattgtcta tgaagcggcg gccgtccacg ccatcgggat cctcgaccag
     3961 aacgacttgc agttcctctg gctgccgctg tcccacgtgt tcggcaagat cctgctcgcc
     4021 ctgccgttgc agatgggctt cccgaccgcg atcgacggca agatcgacaa gatcgtcgac
     4081 aacctggccg tcatcaagcc caccttcatg ggtgccgccc ctcgcatctt cgagaaggcc
     4141 tacgcccgga ttgccgccac ggtggcggcc gagaccggcg tcaagcgtca gctcatggat
     4201 tgggctctcg acgtcggcaa gcaggtgatg gagttgcgtg cgcaaggcca ggcgcccgga
     4261 gcgctgctcg gtatccaaca tgccgtggcc gacaagctcg tcctctccaa gatccgtgag
     4321 cgcttcggtg ggcgggtccg cttcttcatc tcgggttcgg cagcgctcaa cccggagatc
     4381 gcccgctggt tcgggtcggt gggcctgatc atcctcgagg gttacggcct gaccgagacc
     4441 tcggcagcca cttgtgtcaa ccgcccgtcc ctcggtgggt acaactacgg cacggtcggc
     4501 tgggcgatcc cgacgaccga cgtgaaactg gccgccgacg gcgagatcct cgtcaagggt
     4561 ccgggcgtca tgcggggcta ccgcaaccgt cccgatgcca cggcagaggt cttcgacgag
     4621 gacggctact tccgcaccgg tgacatcggc gagatcgacg ccaacggctt cgtcaagatc
     4681 accgaccgca agaaggacct gttcaagacc tcgggtggca agtacatcgc tccctcgttg
     4741 atcgaggcgg ccttcaaggc gcagtgcccc tacgcctcgc agatcctcgt cttcggcgat
     4801 ggtcgcaact acgccacggc gctgatcacc ctcgaccccg acgccatcac cgcgtggggc
     4861 gcagccaacc ggatggccgg caagtcctat gccgagatca gtcagacccc cgaggtgcgc
     4921 gagttgctcc agggctacat cgacaccctc aaccagggcc tcaaccgctg ggagacgatc
     4981 aagcggttca cggtcctgga ccgcgacctg acgatcgacg agggcgagct gacccccagc
     5041 ctcaagctca agcgcaagca cgtcgccgag aagttcaagg ccaacctgga agctctctac
     5101 gactgacagg tccgcccgac gggcgcatca cccgtgacga ggggtacgaa cagattccgg
     5161 cccagccgct gttcgtgccc ctctgcgcat gacgccggta ggatcgacgg atgcctggac
     5221 agtcccgctt cgccgacctg gagccgctgc tcgaccgcgt ccagaagccg atccaatatg
     5281 tcggcggcga gttgaactcg caggtcaagg actggcagtg cggtggccac ggccccggcg
     5341 gcgaggaact cacggtccgg tgggcgctga tgtaccccga cgcctatgag gtcgggctgc
     5401 ccaaccaggg cgtcatgatc ctctacgaag tcctcaacga gcgtccccac gtgctggccg
     5461 agcgcacgta tgccgtgtgg ccggacctcg aggcgctgct gcgggagagc ggcgtgcccc
     5521 agttgaccgt cgacggccat cgcgccgtgg tcgacttcga cctgttcggg gtgtcgttct
     5581 ccaccgagtt gggctacacc aacttcctca ccgccatcga cctggccggc atcccgctgc
     5641 actccaagga tcgcggcgac ggcaccccga tcgtcatcgc cgggggccac gcgtcgttca
     5701 accccgagcc gatcgccgac ttcgtcgatg cggtcgtcgt cggtgacggg gagcaggcgg
     5761 tgctggagat caccgacctc gtggccgctt ggaaggccca gggcagcccc ggaggccgcg
     5821 acgagctcct catgcggatc gcgcgctcgg gtgttgccta cgtcccgtcc tgctacgagg
     5881 tggattactt ccccgacgga cggatccgcc gggtcgcgcc gcggaccggg ctgaccggca
     5941 taccgtggcg ggtctccaaa cacacggtca ctgacctgga cgcgtggccc tacccgaagg
     6001 ccccgctcgt cccgctcgcc gagacggtcc acgagcggat gtcggtggag atcttccggg
     6061 ggtgcacccg aggctgccgg ttctgccagg cggggatgat cacccgcccg gtgcgcgagc
     6121 ggtcgatcac cggcatcggc gagatggtgg agcgggggct cgcagcgacc ggcttcgaag
     6181 aggtcgggct gctgtccctc tcgtcggcgg accactccga gatcgctgag gtgaccaagg
     6241 ggctcgccga ccgttacgag ggcacccaga ccggcctgtc gttgccctcg acccgggttg
     6301 acgccttcaa catcgacctg gccaacgagc tcagtcgcgg ggggcgccgg tcgggactca
     6361 ccttcgcgcc cgagggcggc tcggagcggc tgcgcaaggt catcaacaag caggtctccg
     6421 agcaggacct cgtcaacacg gtcgcggcgg ccttcgcgtc gggctggcgg caggtgaagc
     6481 tctacttcat gtgtgggctt ccgaccgaga ccgacgagga cgtactgcag atcgcctcgc
     6541 ttgccaagca ggtcatcgag accggtcgcc aggtgagcgg gcgccgcgac gtccgatgca
     6601 cggtgtcgat cggcgggttc gtgcccaagc cgcacacccc cttccaatgg gcggcgcaac
     6661 tcggtgccga agcgaccgac gcccgcctgc agttactgcg cgacaccatc cgcgcagacc
     6721 gccggtatgc cgcggcgatc ggcttccgct accacgacgg gcagccgggc atcgtcgagg
     6781 gcctgctctc ccggggcgac cggcgcgtcg gtgccgtcat cgaggccgtc tggcgcgacg
     6841 gcggccgatt cgacgggtgg agcgaacact tctcgtacga gcgttggatg cgggccgcgg
     6901 agaccgcctt cgccgagtcg ccggtctcgg tcgactggtt caccacccgt gagcgcgacc
     6961 ggatcgaagt cctgccctgg gaccacatcg actcgggcct cgaccgcgac tggttgtggg
     7021 acgactggca ggacgcgttg ggggagcacg aggtcgccga ctgccggtgg agtgggtgct
     7081 tcgactgtgg ggtctgcccc gagatgggca cccagatcga gatgggtccg accggtcgaa
     7141 cgctgctgcc gttggcggtg ctcagcccag accgcgcgcg gttgctcgaa acctcaccgt
     7201 gagctgggcc ggcgacacgc aggggtatgc cgtccgccca gccgggccgg acgacctcgc
     7261 cgcggtcact gactgccgtc ggcgctgggt ggccgaacgc accgacgcct cggatcccgg
     7321 cttcgaggac cggatggccg actggtgggt ccgtgaggcc gatcggaggg ccacctggct
     7381 ggcctgggcg ggggagcagc ccatcgggat ggccgcgacc gtcatcttcg agcggatgcc
     7441 cgcaccgggg gcgccgaccg cacggtgggg ttacgtgtcc caggtgtggg tcgacgcggc
     7501 ataccggcga cgtggggtgg cgagcaccct catgggtgcg gtcatcgatt gggcgcgacg
     7561 cgaaggtctc gtgcgcctcg tcctcaatcc cagcgacgag tcccgcccga tgtatgccgc
     7621 gctcggcttc cgcgaggcga gcgacctctt gcggctcgat ctgtccggcc ggtgatcagg
     7681 ggcgtttgag cagccgccgg acgagggcga gcggtgtgga cttcgtccgc tgcggtggga
     7741 ccgggccgag cagggcgcgc aacgcgccca ccacctcgtc gacgaagtcg tccacggtcc
     7801 gctcgtcgta accggagcgg acggtggtgg tgctgaactc cgctttggcg agttcgtcgg
     7861 ccagttgcgg cttggcgacc ccgcgttcga gctcggtggc gaccttgcgc agcaaccagt
     7921 cgacgtcgta acggtcgtaa cccttgtggt acctgatccg ggggaaggtc gttgcccgga
     7981 tccgggccgc aaggtccgcg gccgacgggg tcgacggggg cggagtgggc gcagcgggtt
     8041 ggctcgtcac gggcggtcga cgatcagatc ggatggccga ggtgagccag cgcctggcgc
     8101 aacagcaccc catgaccgcc gaccatctcg ggcgtggtag ccaactcgct ggcctctgca
     8161 ggggtgagcc aggcgaggtc cagggcgttg ccttggggct ggcagtcgcc catgaggacg
     8221 acgacgtagg caagcgccac ggcgtgctgg cgggggtcgt ggaagggcgt cacaccgggc
     8281 gtggggaagt actcagcgac cgcgaacggt tgcggagcga tcgggacctg cggcaaggcc
     8341 atcgggccga ggtcacgctc gatgtggcgc agcaccgcat cgcgcagccg ctcgtgatag
     8401 agcacccgtc ccgagacgag ctcccgttgg atctcgcctg tttcgctggc gcgcagcagc
     8461 agcccgatct gggtgacttg gctgcgttcg tcgacccgga ccggcaccag gttgacgtag
     8521 aggatcggca accgttcccg cgcgtcgcgc agctggtcac gggtgagcca tccgctgcgg
     8581 gtctcgatct cctgcatggt cccgattctg tcagtcgcgc cgccaccagg acgagcgccc
     8641 ggaggctagt agaccttgcc cgggttcatg atcccggacg ggtcgaagag ggccttgacg
     8701 gcccgctgcc gagcgacctc ggcggcaccg agctcccact cgaggaatcg gcgcttttgc
     8761 acacccacac cgtgctcgcc ggtgatggtt cccccgaggt ccagagccgc acgcagcaac
     8821 tcctccaagg cggcctccgc gcggtgccaa ccgtcgacat cgccgtgttc gaagaacagg
     8881 gacgggtgca ggttgccgtc acccccgtgc cccgacaggg tgatctccac gcaggtgcgc
     8941 tcgctgatct cctgcccgat ccgcacaaag tcgcccagtc gggcgacagg gacacaggcg
     9001 tcctcggcca gccgtccgcc cttggcctca agggcgtagt tgagcatccg ccggcccttc
     9061 atgaggagtt cggcctcggc ggcgttgtcc gcaacagcga tctcagccgc gcccaggtcg
     9121 gcgagcgtcg cggcatacct ctcgacgtcc tccgcggcgt ggcccgggcg gtcggactgc
     9181 acgagcaggg ccgcctcgca gcccgagggg aagccgaagt cgttcatcgc ctggatggcc
     9241 gcgatcgagg cgcgatcgag gaactccagc agcgccggtc gatggcgatc ggcgcggagc
     9301 gcgaggactg cgcgggcggc ctcgtccagc gagtcgaagg tcgccagtgc agtgagcgcg
     9361 aggtccggcg ccgggatcag gcgcaccagc gccgacgtga cgacgcccag ggtgccttcc
     9421 gagccgacga acaggccggt gaggtcatag ccggcggtgc tcttcgccgt gcgcttcccg
     9481 gtgcggatct gctcgcctcc ggggaggacg atctccaacg acttgacgta gtcggcggtc
     9541 acgccgtact tgatgcagca caggccgcct gcgttggtgg ccacgttgcc gccgatcgtg
     9601 caggtgtccg ctgacgccgg gtccggcgga tagaagaggc cggcttcgcg gaccgctgcc
     9661 ttgaggtcgg cgttgacgac gccgggaccg acgcgggcat gcccctcgac ggggtcgatg
     9721 tcgatgcccc gcaaggcttc cacgttgagg acgatggcgc cctcggtcgc gcaggaggct
     9781 ccggtgagcg acgtgcgggc tccctgaggg accacgggta tgccgtgcgc ctgcgcctcc
     9841 tccagcacgg cgacgacatc ctgggtgtcg cgggcccgca ccaccgtgaa ggtctcgggg
     9901 gcgctcgcac cgtaggagga gtcgacggca tatgtcgcgg caatgtcggg gtcgcgaagg
     9961 acggcatccc cgaggagggg gtcaaggcgc cgcaaggaag caggcatggg tctgacctta
    10021 acgtcagatg agacgtggct cacgagccct gcaggcttgg tctgacgaga gtgtccaggt
    10081 acgggatagt tgtgccatgt cggcctcgta cggtgagttg atcgccgcac tcgaatcgct
    10141 tccagccaac ccacggatcg tcgccagcgg caacggcgcc attccgttcg agttgctgca
    10201 catcgtcgat cacacgctcc ctgcgttcac cctcaacatg ctcgctgcgc atgccggcct
    10261 gccgctgcgc gagggcgtca cccacgagac gtcgttcgtc ggcgcgggga tgcgtaagag
    10321 cccgcacttg tcctacgttc cggcgcgcct gtcgatggtg ccgctgctgt tctcgcggac
    10381 cctcccgccg gacgcggtca tcgcgcacac gtcgttgccg cacaacggca agatctcgct
    10441 tggcatcgaa acgaacgtcg ttgtcggggc gatcgaggcc tgcaaggctc gtggcggcaa
    10501 gatcatcgcc caggccaaca agcagatgcc ctacacgttc ggcgactccg agtacgacgt
    10561 cgccgacttc gacatcatct acgaggtcga cctgccggtc gcgaactacc agggccgcaa
    10621 gacggtgtcg gccgactcga gtgacacggc gatcatcggt gcattggccg ccgatcggat
    10681 caaggacggc tcgaccctgc agttgggcat cggcgccatc ccggatgcgg tgctgcccga
    10741 gttggtccac aagaagcgcc tggggatctg gaccgagatg ttctccgacg gcgtcctgag
    10801 cctcctcgac gccggcgcga tggacatgga tcggccgatc gtcgcgtcct tctcggtggg
    10861 cagcgcggag ctctacgagt ggatgcacct caacgagcag gtcgtcatgc gccgcaccga
    10921 gacgaccaac aacccggcca acatcgctct gcagccgcgg atgacatcgg tcaacaccgc
    10981 gcttcaggtc gacctcttcg gccaggccaa cgcatctcgc atcaaggcac gcatccactc
    11041 cggcttcggc gggcagaccg acttcatcgt cggcgcgctg cactcgcccg gcgggcaagc
    11101 gatcatggcg ctgcgttcct ggcacccgaa ggccgacgtc tcgacgatcg tgcccctcat
    11161 cgaagagcca gtgacctcct tccagcacag cgcgatcatc accgagcagg gcgtggccga
    11221 gatcttcggt catgaccagg cgcaccagtg ctggtcgatc atcaacaagg cagcccaccc
    11281 gagcgtgcgc gacgagctct gggaagaagc gcacgccctg ggcctggcct gagcggtcgg
    11341 gggagcggcg tgagtggcgt gagcggtcgg gcgccgactc gcgacgagtg gcgggtcttc
    11401 ggctggtggg cgctggtcgg cctcgggtcg atctgggcca cgtggacgct gctgagctat
    11461 ggctggatca tcgcggtgat cacctgcgcc ggagcactca tgctcgccaa ccgcgaggaa
    11521 ccggggcgct cggcgtgggg tttcgtcacc ggcatggggc tggtgccgtt gtggatcggc
    11581 tggaccaacc gggccgccac gacagcggga ctctcgccgt cggtctggat cgggtcaggg
    11641 gccgtgaccg ccctggttgg agtgctcatc ttcgtccgcc ctcgccgcac gacttaggct
    11701 gacgccatgc tgcgcgtggg gctcaccggg gggatcggct cgggcaagtc cactgtcgcg
    11761 gccctcctgc gcgagtgggg ggccgacgtc accgacgcgg accgggtggc gcgcgagatc
    11821 gtcgaaccag gtatgccgcc cctggcggca atccgagaac gcttcggtcc caacgtgatc
    11881 caggctgacg gcaccctcga ccgagcgggc ttggcggcag tcgtcttccc tgatcgcgag
    11941 gccctgaggg cgctggaggc gatcacgggt cctgccatcg cggaccgggt cgcacagttg
    12001 cgggggagtg tccctgtcga tcggatcgac gtctacgaca tgccgttgct cgtcgagaag
    12061 ggcctgtggg tccacgagca cgtgacgatc gtcgtcggtg ccgacgccga ggttcgggtg
    12121 gcgcggttgg tcggccaacg ggggctgtcc gagtcggacg ctcgggcgcg gatcgccgcc
    12181 caagcgagcg acacccagcg tcatgcggca gccgatgcct ggatcgacaa cgggggttcc
    12241 cgggaggaca cccaggccca ggtgcgccga ctctgggacg cacggttggt gcccttcgat
    12301 gccaacttgc gcggcggcat acgggctcgg cgttcggaca ccactacgtt ggccgagccg
    12361 gatcccgggt gggccgacgc tggggcgcgc gtcgttgcgc ggattgcggc cgccttgccg
    12421 ggccagggga tccgcgtcga tcacatcggg tcgacgagcg tgccgggact cgtggccaag
    12481 gatgttgtcg atgtccaggt gggtgtcccg cagttggcgg acgccgatgc gtctggcttc
    12541 gccgctgctg tgcgtcaggc cggctacctc ctggtcgcag gcaacgacca ggaccacccg
    12601 cacccctccg atgccgcacc ggctcactgg cagaagcggt tctacggcgg gtgcgatccg
    12661 gggcggatcg ttcacctcca cgtccgcgag atcggctctg cgggttggga gttcgcgctg
    12721 gccttccgcg actggctgcg cgccgagccc agcgcgcgcg aggagtatgc cgccctcaaa
    12781 cgggagttgg ccgctcgcca ttcggtgacg cgggactact ccgcggccaa ggagccgtgg
    12841 ttcgacgcgg catacctgcg agtccaggag tgggcgcgag aggtgcgctg gacgccctag
    12901 cgctcactgg gcctcagccg acgccgtgga tccccagaac cgccggaagg cagccgtcgc
    12961 gtctgggtgg tcctgcaccc gatggccatg gccgggcagg acgagtcgcg tggcgccatg
    13021 tctctccaac aggtgggcca cctcgtcata gatgtcccgg gttccgctgg tgaccaccag
    13081 cgtcgggacc cgaccgacga tcgtcgggtc gatggcaatg agccaggccg gggtgaacgc
    13141 ccgtaaccgg gccgccgacc gagccaactg gtccggcgtg aagtccggga ccggcatacc
    13201 cacggctgtg gcgaaacccg ccgagagctc cagatcggtg aaggtcgggt cgctccggcg
    13261 ctcgaagagc ggcgcgagag cggccacgtg cgcgtcggtc tcgggccacc ctgccgtcag
    13321 cccgacgaac ggcggctcgc acaggacgag gctcgcgacg acgtccggcc gctcggtggc
    13381 ggcgagcacg gccgacacgc agccgtagga gtgggcgacg aggtggccgc gaggcgccag
    13441 cgcgtcgatg acgaccggct ggacgtggtc gggatggtca ccatcgtgga cccggcggac
    13501 gaagtggacc tcgtcgccga gggccgccgc ctgcaggggc caggccttgg gacccgcaga
    13561 tccggttccg tggaggaaga cgatggacac ggcccgagta tgccgtgcgc gcctggggag
    13621 cggcatacct ggtgcgcgcg tggggtgggt gtcagtgggg cgacgtaccc tgctcactat
    13681 gcgtccgaca accgatctgc agcggcgagt tgccccgttc gaggtgatct ccgacttcga
    13741 gcccagcggc gaccaacccg atgccattgc cgagttggcc cggcggatcc gggccggggt
    13801 caccgacacc gtgctcctcg gggcgacggg cacgggcaag tcggcgacga ccgcgtggct
    13861 catcgagcag gtgcagcgac ccaccctggt gatggctccc aacaagaccc tcgccgcgca
    13921 gttggccaac gagttccgcg agttgttgcc caacaacgcc gtcgaatact tcgtcagcta
    13981 ctacgactac taccagcccg aggcctacat cccgcagacg gacacctaca tcgagaagga
    14041 ctcgtcggtc aacgacgagg tcgagcggct gcggcactcg gcgaccaact cgctgctgac
    14101 ccgccgtgac gtcatcgtcg tggcgtcggt gtcctgcatc tacggcctgg gcaccccgca
    14161 ggagtacatc gaccggatgg tggcgctcaa ggtcgggcag gagatcgagc gtgatcagtt
    14221 gctgcgccgg ttcgtcggca tgcaatacac ccgcaacgac ctggccttca cccgagggac
    14281 cttccgggtg cggggcgaca ccgtcgagat catcccgatg tatgaagagc tcgcggtgcg
    14341 cgtggagttc ttcggcgacg agatcgagcg gatctacacc ctgcacccgc tcaccggcga
    14401 ggtcgtgcgc gaggagcagg agatgtatgt cttcccggcc tcgcactatg tcgccgggcc
    14461 cgagcgcatg gagcgggcga tcggcggcat cgagtcggag ttggagaccc ggttggccga
    14521 gctcgaccgg cagggcaagc tcctggaggc ccagcgcctg cggatgcgca ccggctacga
    14581 catcgagatg atgcgcaccc tcgggttctg taacggcatc gagaactaca gccggcacat
    14641 cgacggccga gaggccgggt cggcccccaa ctgcctgctc gactacttcc ccgaagactt
    14701 cctgctcgtc atcgacgagt cgcaccagac cgtgccgcag atcggggcga tgtacgaggg
    14761 cgacatgtcc cgcaagcgga tgctcgtcga ccacggcttc cggctcccct cggcgatgga
    14821 caaccgaccc ctgaagtggg aggagttcct tgagcgcatc ggccaaaccg tctatctctc
    14881 agcgacaccc ggcgactacg agatggccaa gtcccggtcg ttcggccctc cggtggagca
    14941 gatcatccga cccaccgggc tgatcgaccc cgagatcatc ctcaagccca ccaagggtca
    15001 gatcgacgac ctcatccacg agatccgcac ccgggcggcc cgcaacgagc gggtgctcgt
    15061 caccaccttg accaagaaga tggccgagga cctcaccgac tacctgctcg acaagggcat
    15121 ccgggtgcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng agttgctcgg
    15241 ctccggccgc tcgcgctccc gcggcaaggc atcgggtatg ccggccggcc gcgaccgcgc
    15301 gggccgcggc gcgatcctcg cggacgtcgg cgtggcgggt ggcaagcggg tcgagggcct
    15361 gcccacctcc gacctggccg ggctgattca ggagctcacg gcccagatgc acacggccgc
    15421 ggccgagttg cacttcgagc tcgcagcccg gttgcgcgac gaggtctcgg agttgaagaa
    15481 ggaactgcgc cagatgaccg aggcgctgcg ctgacgacga gagcaacctc acgctccgtt
    15541 ctcgaccccc aagtaaggca cccctaactt cgctcgatac catgcaaggg tgaccacgag
    15601 cgaggtgtcc ctggcggcct ggccgatcgg cgccccaggc gtgctcgcgt cagccgccgc
    15661 cccagcgggt cctccctccg tcgccgcccc caccgacctg ctgacccatc tggcgagact
    15721 ggggctgcgc ctaggcatga ccgtcactcc actcatgcgc acccctgggc gcgggctggt
    15781 tctcgcggta ggcgatctac gcctcgcggt cgatcgggcc agcgccgacg ccttgcgagt
    15841 gcacgcaccc gggcactcga gcccgaggtc gacgtccaac acccccgatc cgacgccgac
    15901 ggtcgaggtc tgacatgggc aacgccgggc ttgagaccgg gggagcagtg accgccgcac
    15961 ccaccccctc ctgccatgcc ccggccgccc cccaagcccc cacgggctcg ctcgtcgtcg
    16021 ccctcgcggg tgcccccaac gttggcaagt cgacgctgtt caacgccctc accggagcgc
    16081 ggctgacgat gggcaactgg ccggggacca ccgtcgaggt gtcccgcggc cggtggcaac
    16141 ccgatgccgg atcggtgacc tacgagttgg tggacctccc cggcgcctac tccctcgacc
    16201 cgatctcgcc ggacgaggag ctcacccgcg cggtcctgct cgaccccgac ccggccgccc
    16261 ggccacacgt cgtcatcgtc gccgccgatg cgacgcacct ggcccgcagc ctctacgtcg
    16321 tcgctcaact gcgtgagcac gcccaacgca tcatcgtggc gctgaccatg gtcgacgtcg
    16381 cggctcgacg cggcatcacg gtggaccccg gcggcctcga acgcgccctc ggcatcccgg
    16441 tcgtggccgt cgaccctcgc cgacgcaccg gcctgacggc cctcgccgaa cgggtaaccc
    16501 agatcgccgg tgcgcccgag ccggcaccgc gcctccagga caccgaaacc cgcggcgagc
    16561 gggacggcga cgccgacgaa ctcgaccgtg ccgaggaacg cttcggttgg gtccaggacg
    16621 cggtggatcg ggcccagacc cgagaagccg ctgcacggca tacctggacc gaccgggtcg
    16681 acgcggtcgt gctctcgcgc gtctggggac cgatcgtctt ccttgcggtg atgtggctcg
    16741 tcttccaggc cacgacgacc gttgcggccc cgatccagga cgccctcgac gccctcttca
    16801 ccggcccggt gagcgagggc gcgcgggcag cgttggcggc cctgcacgtt gaccacccgt
    16861 tggtcacggg attggtcgtc gacggcctca tcgccggggt cgggatgctg ctcaccttcg
    16921 tcccgctcat ggccgtgatg ttcgtcctgc tggcgctgct cgaggactcc ggctacctcg
    16981 cgcgggcggc cgtcgtcacc gatcggacga tgcgcgccct cggcctgccg gggcgtgcct
    17041 tcctgccgct catcgttggg ttcggctgca acgtgcctgc catcgcggcc acgcgggtgc
    17101 tcggtggagc caagcaacgg gtgctgaccg ctctgctcgt ccccttcacc tcctgctcgg
    17161 cccgcctcac cgtctacgtc ctgctcgcga cgacgttctt cgatcggtat gccggcaccg
    17221 tcgtcttcgg catgtacctc ctgtcgatcc tgctcatcgt cggcgtcggt ctcgtcctgc
    17281 gccgcaccct gtggcggagc atgctcgccg aaccgctcct gcttgacctg cctccctatc
    17341 aacgacccac ggcccgggtc acctggtcgg tcacctggac ccggctgcgg gggttcctgc
    17401 gcaccgccgg cggcatcatc gtggccacgg tcgctgtcgt gtggctcctg cagagcgtcc
    17461 cggtccgcgg cgagcacgcc ttcggggcga ctcccgtcgg ggacagcgcg tatgccgccc
    17521 tcgccggcgc cgtcgcgccg gtcttcgagc ccgccgggtt cgggcaatgg caatctgcgg
    17581 gggcgttggt gaccgggttc gtcgccaagg aggctgtcat ctcgtcgtgg gcgcagacct
    17641 atgcgacgct cgacgacacg ggtgccgccg acgaagggcg cctggcgcag gcagtccgcc
    17701 aagacttcga gatctcctcc ggggggcatg gcaacgcggc cgccctggcg ttcatggtct
    17761 tcctcctggc ctacaccccg tgtgtggcga ccctggccgc gcagaagcgc gagatcggcg
    17821 tgcgatgggc cgcgttcggg gtcggcatgc agttggccgt ggcctgggtg ctctctgtcg
    17881 tggtcttcca gatcgcgagg ctggtgggat gaccctgccg agtcaagcca gggccaagcc
    17941 gctgcacctc gtcctggccg ccatcggctc cggggtcggc agccgaggtg agttgacgcg
    18001 gcataccgga ttggcgcctg acgtcgtcga tgccgccgtg gaccatctcg tggctaccgg
    18061 tcgcctgaca aacacggcgc tcgggggcgg gtgcgccacc ggcggttgtg gcgcgtgccc
    18121 gagcgggcgg gccgatgccg ggcccgggtg tggcgtggcc gaccctcgcg atgcgcgggg
    18181 gccggtcgtg ctcactctcg ccaccctcgc cacccgcagt cgcggctgag gggcgtcgcg
    18241 tcaccagtcc gggtggtcgt cctcggtggg gtcgctgatc gcctggccag ccgtcagttc
    18301 tgcggctttg cgatcgcggg acgacttgag gaggctggca atcgtcgtca cgccgaggat
    18361 gaggacgatc gcgcccaacg acaccgagat cgggatgtcg ggtatgccgt gcaacggctg
    18421 accgccgttg atgaacggca ggttgttctc gtgcagggcg tgcaggatca gcttgacgcc
    18481 gatgaaggcc aacagcacgg ccagcccgat cgacaggtag accaggcgct tgagcagccc
    18541 gccgatgagg aagtagagct ggcgcaagcc catgagggca aagatgttgg cggtgaggac
    18601 gaggtagggc tccttggtga gcccgtagat cgcggggatc gagtcgaggg cgaagagcag
    18661 gtcggtcgtc cccaacgcca tgatgacgat gaacatcggc gtcaccagcc gcttggcgcc
    18721 ctcgtggacg aagagcttgg tgccgtggaa ggaactcgtc gccgggaacc gtcgctcaac
    18781 gaactgtagg aaggcgttct cctcatattc gtcgtcgtcg ctctcacctt cccgggccaa
    18841 cttccaggcc gtccagatga ggaaggcgcc gaagatgtag aacacccagg cgtaggagtt
    18901 gatgaccgcg gcccccagcg cgatgaacag cccgcgcagc acgatggcga tgatgatgcc
    18961 caccatgagg gccgactgct gcagccgctc gggaacccgg aagttggcca tgatgagcag
    19021 gaagacgaag aggttgtcca ccgacaggga gtactcggtg agccagccgg catagaactc
    19081 tccggcgtac tgcccgccga agtcccacca gatgaacagc ccgaacaaca gcgccagcga
    19141 gacatagacg accaggtagg tcgtgcactc gcgcatcgac ggcacatggg gtcgacgggc
    19201 cacgacggcg acatcgagca acagggccac tgttgccgca ccgatagtga cccaccacac
    19261 caggggagtg acgtccacga agcaccttcc gttggggatg tccctgagac tctacccagc
    19321 gttggggcgt tgcttgcgcg gcagcgccgt cacgaccgac tcgtaggacg cgtcgatcgc
    19381 ctcgaggagt tcgtcgtcag ggatggcccc cgccaaccgc agggtgttcc atccgtagcg
    19441 accgatatag gccatgacgc tggcgtcctg cgggtagcgg tgcagccact cgtcggcctc
    19501 gtcccgccct cgcccgagtt tgagccccac cgcgtcctcg ccggtgaaag cgaagatctt
    19561 cggaccgacc ttggcgacga catcgccgtc ccatggctcg tccggccagg cgccgggctt
    19621 ggccaggcaa taggcgagca actcctcacg cgtcatggcc gcgaggctaa ctcaggagcg
    19681 ctgcgtcgtg ccgggcagcg ggaggcgtcc cacggcatac cgggtcgcct cgcggtccgg
    19741 ccccaacgcc tcctcgacga tcccgcgtcc cgtttcgggg gcatggagca tccgcccagg
    19801 cgcgctgacg atccccacgt ggtggactgc gcgccccggg tgggcgaaga agtagaggtc
    19861 accagggcgc tcctcaccga acgtcaccgg gtatgccgcg gcctcctgat cgtcggcgtc
    19921 gcgcgggacg accacgccca accgacgcca cacgtggtgc accagccctg agcagtccag
    19981 cccgagcggt gaggtcccgc cccagaggta gggcaatccg aggtgttcag cagccagggt
    20041 gaggacgtcg gctccgggag cgtcggcgac cggaaggccg ggctcgtcac cggccgagcc
    20101 ggcgtcctcg agatggctgc ggcgcaccca gcccgggtaa ccgcgggggt ccaaccggct
    20161 gggctgccac gggcacacca cccggaccca gccatcggcg ctcacgtcgc tgatcgggag
    20221 gaccggctcg ccgagcacgg cctgactctc gacccggccg tgcaggccga gccggccgtc
    20281 gccgctctcg tcgtcctgcg ggcgggcgtc gaggacggcc aaccacctgg ccgggtcggc
    20341 ctggtcggcg atcgccgcgg catcgaccgg ccggggggca tccggcgagg tccagagggt
    20401 cgtcacgggc acggacacac gcagcagggc catcgcccca tcttcacatg cccgatactg
    20461 ccggtcatgg atcccgctgc ggtgaccgct cgtctcgccg atctcgtggc cgcggcccca
    20521 ggccgtctgt cggtcgccgt acgtgctcgg gacggctacc ggttcgacca cgactcggcg
    20581 cgagtcgtcc ctgcggcgag caccatcaag gtgcccgtgt tgctggccta cctcgcctcg
    20641 gggatgccgt gggacctgcc cgtggcgctg ccggccgccc gggtgggtgg gtgtggcccg
    20701 ctcgcgctgc tgccctcggt gaccagcctc cccgcggggg aggcgttgcg cctgatgatc
    20761 gcgctgtccg acaacgacgc caccaacgcg gtgctcgggc tcgtcggtgt gggcgaggtg
    20821 gcgcggttgc tcggacgcgc ggggacgcgg tataccgtgc tgcggcgccg gatgatggac
    20881 caggtggcgg ccgctgcggg tcacgagaac gagacctgcg ccgcggacct ggccgagttg
    20941 ctggccgccg tgcgcgccgg gtcgatcctg ccccagcgcg aggccgccat ggcgctggcc
    21001 gtcctgcgcg agcagcagtt cgacgagggc ctgcccgcct atctccctga gggcctgcgc
    21061 tgcgcgtcga agaccggcag ccttcccggg gtgcgccacg acatcgcggt catcgagagc
    21121 ggtgacgcgt gggtcgcggt cgccgccctg gccaccgacc tcatggacgg cccggtcgat
    21181 cggggcacgt cgatctaccc cacgtatgcc gcgatcggcg agacggtcgc cgccctcgtc
    21241 accggctgaa cggaggccga tcgggcgcca acgcggtcgc tcaggtgatg ccgacgatcg
    21301 gcagacgcag ggctgccgga gccgacgccg ggacgatcgg cttcgccggc tcgaccggtg
    21361 cgatcccttg gtatgccgtg cccagcgccg gccgcgcgtc ggtctggccc ttgttgggcc
    21421 agtaggccat ggcccgctcg gcctgagcgg tgatggtcaa cgacgggttg accccgaggt
    21481 tggccgagat cgcggacccg tcgacgatgt gcagaccgtc gtaaccgaag acccggtggt
    21541 aggcatcgac gacgccgtgg tcggcgtcct tcccgatcgg gcagccaccg atgaagtgcg
    21601 ccgtcagcgg ccgatggaac tgctcgccga tgttgccggc tgggaagccg ccgacgttcc
    21661 gagccatgcg tcggaccgcc tcgttcgcca atgggatcca ggtcggattg ggtgcaccgt
    21721 gaccctgctc ggagctcaac gtgatcttgc cggtgacccg attgcgcttg gggaaggtgt
    21781 tgatcgagtt gtccagcgac tgcatgacca acgcgatgac ggtgtgctcc gaccagtgtt
    21841 tgaagtcgta gagccggcgc acggtggatc gctcgtgcca cgactggcgc agccagaccc
    21901 gccaccgggg cgtgtcgccc aggtcgtcgg tgagcaccga ggtgagcagc gacatgacgt
    21961 tggagccccg gccatagcgg accggctcga tgtgggtgt
//
LOCUS       DAORPW010000028        21660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_403_length_21660_cov_710.996112, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000028 DAORPW010000000
VERSION     DAORPW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 21660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21660
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_403_length_21660_cov_710.996112"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLB86_04055"
     CDS             complement(<1..596)
                     /locus_tag="PLB86_04055"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HRC64341.1"
                     /translation="MLRLGIDVGDTNTDAVLVDESRVVAAASVATTPDINGGILRAIT
                     GLRTGPGFEHSQVAAVMIGTTHFSNALAEGNDLAQTAAIRFGLPATSAFPPLVDWPEG
                     LRRAIGGHGYLCHGGNEYDGSRIDDFDEREVRDAVGRALDAGARSFAISAVFSPVDAE
                     FELRAAQIVSEVAPGAPISLSHEIGRMGLLGRENATII"
     gene            951..2009
                     /locus_tag="PLB86_04060"
     CDS             951..2009
                     /locus_tag="PLB86_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRC64342.1"
                     /translation="MKRHLIPSLIVGLSALALVACSTGSTGSTGATAPAPAATAAPAN
                     TGGALAGKKVLYVDALPGNALLDGLAQGLAAELTKQGAKVARVFQLNAQNQIDLAAAN
                     QRINEGIAQGVDAIVTFPLDANAIRPGVDAATKAGIPIFTFQALGGLDVTGKVRFPDE
                     ARGEATGKGVAALAGGTGEATVMSGQPTDNVEAAVRGAVKGLTGGGMTLVGDPNNQRN
                     LKDDAPEAQRIAMTIFQKNPNLRVLVVYNSASATGAIAAAQQAGVADKVLIATMAGED
                     TNVDQVKKGTLALTYDLNGLDYGKVMGQMVAKALSGTKMTNEVVDAPTGTIYTKDNVG
                     TYIPWSQRLTYADIPSKF"
     gene            2014..3570
                     /locus_tag="PLB86_04065"
     CDS             2014..3570
                     /locus_tag="PLB86_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC64343.1"
                     /translation="MNASPRPELPESAVTVTSVTKHYGVTQALAGVDIAFRRGAITAV
                     VGANGSGKTTLLKVIAGLLRPTSGRVQGPDGRELQGLTSAQESGVVLVPQEPTLAGHL
                     SVWQNVSLGRPEAARGPFVNDRAARELAARRISGLLPPDVLERTTSSLSKSSRQLVQL
                     AAAMAKEPLVLMLDEPTAVLDEDGVVALHALIRGFVGAGGTVVIVSHRLRDVLELADD
                     VVVLRNGLVSHAGPVTADTEHHVVRLLSAEERASRPQHRPDRGHVVLQARDLRGWRGL
                     SVDDFAVHAGEIVGIAGQSGSGRSRLAAVLAGAYAAGGEVSVEGQKVRLGSIRSAQAA
                     GICYIPEDRQVNAILGNQSVAANLLVGREDPELHNGPFRNRPAEKAESEILMERFEVR
                     PPDPDRLAALLSGGNQQKLVVGRALSRPAKVVVADEPTQGVDASARGAIHAALASAAA
                     AGRAVVAVCSDFEELFEISDRIVVLRDGQIVLDQPRKHVTQDEVLAASLGSHLPVADP
                     GSHVSEAHPS"
     gene            3567..4589
                     /locus_tag="PLB86_04070"
     CDS             3567..4589
                     /locus_tag="PLB86_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC64344.1"
                     /translation="MINPNPADLVGQQPAQPATSPQVSDDPSRRRISSATRQQGFFLA
                     VIVAMFLAAMPFLPQFHNGQYLMIQLSSYAAILIMALGQTFVVIQVGFDLSTGAMQGF
                     MGGLVFVLVGWQVPDGLVILAGLGAALAVGVLVHGLLIAKAKINFFIVTLASFTILPS
                     LLRVLLNGSSKPVFSPTLDAMANGSIAGIRTPVIIGAVVFGAMWVLLNRTVFGRNVYA
                     VGSNPEAARLAGVNVDRVAMICYGICALCAGIAGLLLVGSLGAADPSAGLGNEFFALT
                     AVLLGGTRFSGGAGTLFGTLLGLIFLIVLSNILLLSGLPQFWIGAVQGVVLLVAVGID
                     RARRDA"
     gene            4742..6319
                     /locus_tag="PLB86_04075"
     CDS             4742..6319
                     /locus_tag="PLB86_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRC64345.1"
                     /translation="MTLRIGIDVGGTNTDAVVLDGRRVVAATKSPTTEDVTIGILSAL
                     NQVLQGVDTTEVSAVFIGTTHFINAIAQARGLEPVAVVRLATPPQSLLPMIDWPDHLQ
                     GIVGDNFYVCPGGSQFDGTPLNAVDEVALRDVAKKIAESGHRHIALSSIFSTVNPEPE
                     LLAERILLEELPGVTVTRSALVGRVGLLERENATILNASLLDLAGRVIDGLEHAVATA
                     GIDAPILLSQNDGTVMTLDRARLFPIFTIASGPTNSMRGAALLTGIDDAVVIDVGGTT
                     CDVGLLQRGFPRESTVAMSLAGVRSNFRIPDVLSFALGGGTIISDDGQTVGPESVGFR
                     ITEKALLFGGDTLTFSDIAVAAGATPMGDPARVADVSPDTIKAVMAIVGARVVHAVEK
                     AMLRADDTPVIVVGGGGPLLAVVPGLEDLVIPENAGVANAVGAAFAEAGGEVDRVFNL
                     AGRSRVEVLDQAKAEAIAKAVEAGADPDTVRIVDAEDVPLTHLGGGSAVHVRVKAAGP
                     FARSAAMTSAAMTSEGN"
     gene            6321..7400
                     /locus_tag="PLB86_04080"
     CDS             6321..7400
                     /locus_tag="PLB86_04080"
                     /inference="COORDINATES: protein motif:HMM:NF017812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HRC64346.1"
                     /translation="MRIIDEAALNDITLGSTVLGAGGGGDPYVGMLLARDAIRRFGPV
                     PVVDLDEVPDDANVAFIAGIGAPGVLIEKLPRAAEYERVLQTLERFTGVTYDYVCPAE
                     AGGLNAVTPFATAAPRGIPVIDADGMGRAFPHLEMVTPTLYGGKATPCVFIDEHGNTL
                     YLETETNSWAEAFARAAVLASGANAAMALYPMTGAEAKKWLVRGALTMAQDIGQTIRQ
                     ARVAKTSPVHAVLESQNGVMLFTGKVEEVDRRNERGWTMGTATLSGLDDYSGRTMTLH
                     FQNENLAAERDGVVIATSPDLIMAMEIDTGEPIPAEEIRYGYRVAVIGLPADPHWRTE
                     AGIELSGPRRFGYDMDYRPVEEPVA"
     gene            7397..8671
                     /locus_tag="PLB86_04085"
     CDS             7397..8671
                     /locus_tag="PLB86_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC64347.1"
                     /translation="MTRIVLRGGRVFDGTGADAFDADVVVDGDRIVSVGPPAGDGASG
                     GERAGGGSGGSGGSGGSGDTVIDVTGMLIAPGLIDCHVHTVFDGMDLLRVQGRPFSME
                     FFEAAVVLKRLLECGVTTVRDAGGADLGVKTAVETGLIPGPRMAIAISVLSQTGGHVD
                     GWNVHGDNLRLMVPHPGRPDCVVDGVEDMRKRVREVIRAGADVIKVCASGGVMSTRDD
                     PRHAQFSYAELAVCVEEAAATGRSVMAHAHGAAGIKQALRAGVRSIEHGVYIDDECIE
                     LFLQTGAWLVPTLLAPVSLVEAIEAGMRVTPEIEAKARAVSDLHVQAIARAHAAGVKI
                     AMGTDSGVFAHGINHKELAWMVRAGLSPQQSLRAATGSAAELLERSDIGGVAPGKLAD
                     LVVVAGDPWGFDRFAENLQLVMKGGQVVRDAC"
     gene            complement(8802..9851)
                     /locus_tag="PLB86_04090"
     CDS             complement(8802..9851)
                     /locus_tag="PLB86_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRC64348.1"
                     /translation="MRATFIHGAGDVRVADAPDPVIVHPTDAVVRVVRACVCGSDLHP
                     YHAMPPAPGGRSIGHEFIGVVEAVGSEVRTLTVGDFVIAPFAVSCGTCEFCRAGLQTS
                     CSNGGFWSDARVGTAGGQAQAVRVPLADGTLVVAPGVDESTDESLLASLLTLSDVYGT
                     GWHAAVRGQVAPGSTVTVIGDGAVGLMAVLSAQLLGAEQIVLMGRHRARTDLGVEFGA
                     TAVVAERGDEGIAAVRDLTGGGSQIVLEAVGHYPAYEQAIGIVRPGGVISRVGVPQYD
                     QAPVGFGTLFGPNITLTGGPAPVRAYLETLLPGVLDGTVNPGRVFDRTFDLEDIPAAY
                     AAMDAREVLKAMIRP"
     assembly_gap    10010..10109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10110..10646
                     /locus_tag="PLB86_04095"
     CDS             <10110..10646
                     /locus_tag="PLB86_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRC64349.1"
                     /translation="DAITDAPAWVRNGRHDILAMNPLARALYAPVLADPRRPANTTRF
                     IYLQPEAARELFVDYDQVAKDGAAMLRLEAGRNPHDKDLIQLVGELSTRSELFRQRWA
                     SQDVRYHRSGRKRLRHPVVGQLDLDYEAMELLSDPGLVLNMYTAAAGTPTSDALKILA
                     SWVAGQEQLVTELAPSNG"
     gene            10681..11529
                     /locus_tag="PLB86_04100"
     CDS             10681..11529
                     /locus_tag="PLB86_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRC64350.1"
                     /translation="MTDTETPSPRRRAVFLDVDGTYAVFGAAPAGHVAAVRAARDAGH
                     LVFLCTGRPMSMVPAHLLAAGFDGVVAAGGAYAVLGDEVLLDIRFPADLAARTVEVMD
                     SYGVTYLLEAPEAVYTASGTEEQLRRAYRAHLADAPSATTVVSDILASVRPVPAAGAV
                     SFAKVSVVVSSVPVEQLGAEVGEQLAVLPSSVPALGEEAGEIFLADVHKAVGMDAIVR
                     RLGLAREDVIGFGDGLNDLEMLEYAGVGVAIEGSHERLLAVADRTAAGPEVEGLVQAF
                     AELGLR"
     gene            12118..13998
                     /locus_tag="PLB86_04105"
     CDS             12118..13998
                     /locus_tag="PLB86_04105"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HRC64351.1"
                     /translation="MASPTKYDLLAAEVIRGVGGADNVASVTHCATRLRFALKDRDKA
                     DKAAVEATRGVITVVESGGQFQVVIGNTVNNVYEAIVAKGDVKSGGEVAAGGFLAKAI
                     DLVTSIFTPFLWVLAGTGLIKAFLALAGKLSPDFATSSTYAILFAAGDALFQFLPMLL
                     AVTAAKKFKANVYTSLAIAAALIYSATIPVIADAKGVSMTLGAFDAASNLTFLGIPVV
                     MVSYLSAVVPTILAIYAQAKLEHWLERVIPETVRNFVVPLVAVAVVVPLTFLAIGPVA
                     DLIGKGVSGGVNALWSLSPVIGGAVMGALWQVFVIFGVHWGFVPVMIQDLTTQGYSLL
                     TGPLFAAVLAQGAAATAVMIKTRNKELKEIAGPAALSAVLAGITEPAIYGVTLRLKKP
                     FIYGCIGGAIGGGIAAAGGSAAKGFVIPGLITMSAAVDVGNFGLQVAGTVIAMAIAFG
                     LTMVLGFQDIPAKAALDDAAATGEVASEAVSVAAPAIGEVVSLESVPDKVFASGALGA
                     GLAVVPAVGEAFAPVSGTLITATSHAYGIKTDNGLEVLVHIGIDTVKLGGKHFSPKVV
                     QGQRVTAGQTLAEFDIAAIEKAGYNPVVVMIVTNNGPFSAAVPVASGSIRMGETALDL
                     VG"
     gene            14129..15556
                     /locus_tag="PLB86_04110"
     CDS             14129..15556
                     /locus_tag="PLB86_04110"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRC64352.1"
                     /translation="MTSTPETYFPDGFLWGGATAANQIEGAYTEDGKGLSIQDVMPKG
                     IMAAPTSEPTPDNLKLEAIDFYHRYADDIALFAEMGFSVFRFSIAWSRIFPLGDETEP
                     NEEGLAFYDRVLDELERHGIEPLVTISHYETPLHLARTYGGWVNRDLIGFYERYVRVL
                     FDRYGHRVKYWLTFNEINSVIHAPFLSGGIPTPREELTETDLYQAIHHELVASALATK
                     IGHETNPRIQVGCMVLAMPIYPLTSSPDDAIAVMNADHGNLMFSDVHTRGAYPGYALR
                     MFREQGIELDITDEDRELLTNTVDFVSFSYYMSICETADPAQKTAGQGNIMGGVPNPH
                     LPASQWGWQIDPQGLRVVLNQFWDRWQKPLFIVENGLGARDELVEVDGIPTVVDDARI
                     AYLNDHLVQVGEAIADGVNLLGYTTWGPIDLVSASTAQLSKRYGFIYVDRNDDGSGTL
                     ARYRKKSFAWYAEVIRTNGASLVRG"
     gene            15593..>16437
                     /locus_tag="PLB86_04115"
     CDS             15593..>16437
                     /locus_tag="PLB86_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRC64353.1"
                     /translation="MQVVKVFNNNVVLGADGHGGEFVLLGRGLGFRVSPGMDIDATRV
                     ERTFVPSGVTTADRLAALVGEMPLADIELTERIVAMARSSLGSYVTDLVVVPMADHIG
                     FALRRLAEGAPEIEYPLRWEVHYLYPAEVAVARQALDLIELERGVRLPDIEAVPLALH
                     FVNAQFGAGDINATLQMTELLQRILAIIGEEYNVVIDEGAVAVARFVTHLRYLFHREQ
                     QGKKLQQTPAELHEALRAARPREYASAGRIGELLRARFGWEITNDEILYLALHVSRLI
                     DDGSTP"
     assembly_gap    16438..16537
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16538..16765
                     /locus_tag="PLB86_04120"
     CDS             <16538..16765
                     /locus_tag="PLB86_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HRC64354.1"
                     /translation="EQILTAVGGPDNVASLTRCWARLRFELHDAAVVDEAAIGALPEV
                     ALVVTQHGQFQVAMRTGLLEVFDELVALLER"
     gene            16863..17132
                     /locus_tag="PLB86_04125"
     CDS             16863..17132
                     /locus_tag="PLB86_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRC64355.1"
                     /translation="MATRSVIIASSVGLHARPASIFVQAANATGLDVEIGRPGEEPVD
                     ATSILGVMSLGAGHGDEVVLTAEGDGADAALDELVALLSRDLDAE"
     gene            complement(17224..17607)
                     /locus_tag="PLB86_04130"
     CDS             complement(17224..17607)
                     /locus_tag="PLB86_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HRC64356.1"
                     /translation="MIEYLTLEDVLTLIDDLGVGPVRDVGLLDSAVHRPQASVFGVDA
                     YPNIDEKAAALLESLVRNHTLIDGNKRLGWLAIVVFYGLNDITLDAPDDEAYDLVIAM
                     ASGASTYQEAAAHLSRWRVGEHPVP"
     gene            complement(17604..17807)
                     /locus_tag="PLB86_04135"
     CDS             complement(17604..17807)
                     /locus_tag="PLB86_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRC64357.1"
                     /translation="MAMTLRLTPEDEEALTMLAKADGVSKQEATVRAIHEAASRRARG
                     DKMAGLSASARHRYADLLDRLGQ"
     gene            17925..18596
                     /locus_tag="PLB86_04140"
     CDS             17925..18596
                     /locus_tag="PLB86_04140"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRC64358.1"
                     /translation="MRWAVGPTSSKKSGAADRVAAVLREQIVKGLFPPGLRLSEEALG
                     SDLGVARNTLREAFRMLSQERLVVHRLNRGVFVRELTVAEIEDLYRLRSAIEIAALSQ
                     GGLVEPELGPLRAALAEADEAALREDWKAVGTAGLVFHSRLVQLAGSERLSAVMDGLM
                     AELRLAFHMAANVNELHTRYALRNKMVFALLEEGDITGAAVALRDYLTDSEADILQAY
                     SGVRA"
     gene            18704..19255
                     /locus_tag="PLB86_04145"
     CDS             18704..19255
                     /locus_tag="PLB86_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRC64359.1"
                     /translation="MTTATKPPTPSPHVPVGLDWHLAIAQELIARGVSVAAYVPDSRL
                     NGVLAALGERDIRLRNLAREEECVAYAAGQRIAEARPVVLMQSSGLGNALNALGSLAV
                     PYRLGIPLIISMRGTLGEKNPSQVPMGRATTAWLGALGIQSFSLRQASEVGPVISGVL
                     DLAFDAGETAAMLLEPELGGGRA"
     gene            19252..19833
                     /locus_tag="PLB86_04150"
     CDS             19252..19833
                     /locus_tag="PLB86_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRC64360.1"
                     /translation="MNVNDVTRTIMQCRPDAILVSSLGTATSSVRLVTSDGPHFYFGA
                     AMGSALAGALGLAEAVPDRQVVAVLGDGEFLMGASALWSVAAYRPSNLTVVVLADGAY
                     SITGGQPIPAPLHVAAVANALAGTRGVAVSSTTELAATLRSEPTSPDAPGTPLVIEAT
                     ISERTWPGPSPFIDPAATVRNLRASLAQAPAPA"
     gene            complement(19888..20763)
                     /locus_tag="PLB86_04155"
     CDS             complement(19888..20763)
                     /locus_tag="PLB86_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRC64361.1"
                     /translation="MPARQTSGGSLLLVMAAVVSVQFGGAVAATLVPVIGAAGSVVLR
                     LGIGAVVLLAVARPTLRGHSASAWRTVVLFGLVLGAMNWSFYASLDRLHIGVAVTIEF
                     IGPLTLAAVQSRRVRDLLAVAAAAAGILLISQALTVPWGDLDLIGIGLALLAGTFWAG
                     YIVLSQRTGAAFPGLNGLALAMIVALALVLPFGIWSVPLWTGDVLVKGAAVAILSSVL
                     PYSLELLALRQLSQRVFGILLSLEPAVAALAGLIVLGQRLTPTQLVGMALVVVASGLV
                     MGAGGRHTPADGAAT"
     gene            20933..>21660
                     /locus_tag="PLB86_04160"
     CDS             20933..>21660
                     /locus_tag="PLB86_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRC64362.1"
                     /translation="MTIEPAGQHFPHLFAPITVGTMRLRNRVMLPPHASAIGNIYGTD
                     DEAARNIAYFEERARSGVAWIASLSTHIRNHLIPGFDPTGVGAATTGFFRLPFFVERV
                     QAFSDACHALGATVSVQCVHQGGMPHAPSARLSAPVINLVPHVMTHADIAEFVREYAE
                     SARLSMLGRADGFELHLNHDDLHEWFLSPYTNYRTDRYGGSLENRCRFATEVLQAVRD
                     VVGTSMTVGIRLNIRQEAPEGFGVA"
BASE COUNT         3280 a         7496 c         7215 g         3469 t
ORIGIN      
        1 ttgatgatcg tggcgttctc ccgcccgagc agccccatcc ggccgatctc gtgggagagg
       61 ctgatcggcg caccgggagc cacctcggag acgatctgcg ccgcccgcaa ctcgaactcg
      121 gcgtcgaccg gcgagaagac cgccgagatg gcgaaggacc gggcaccggc gtcgagcgct
      181 cgccccaccg cgtcgcgaac ctcccgctcg tcgaaatcgt cgatgcggct gccgtcatac
      241 tcgttgccgc catggcagag atagccgtgg ccgccgatcg cgcggcgcag cccttctggc
      301 cagtccacca gcggcggaaa ggcgctggtg gcgggcagac cgaagcggat ggcggcggtc
      361 tgggcgaggt cgttgccttc ggcgagcgca ttgctgaagt gggtcgtccc gatcatcacg
      421 gccgctacct gggagtgctc gaaccctggg ccggtccgca gcccggtgat ggcgcgcagg
      481 atgccgccgt tgatgtctgg tgtcgtggcg acgctggccg cggcgacgac ccggctctcg
      541 tccacgagga ccgcgtcggt gttggtgtcg ccgacgtcga tccctagccg cagcatgacg
      601 gccgcgccgc gggccggcgc tcggtgctcg ggtccatccg gccctccacg ggatcagggg
      661 ttcgacccac gttagaggta tgccgtgatc ggcgtcacta tgcgggtagc ccagattaga
      721 tgggattcct aggcctagcg ccctaggcag cggaaggacc cggccccaca tccatcgcgc
      781 ttggcgtcga gtcgacggac ccgtgctggc actgtgccta gccgtgacta acttcctagg
      841 cgcatcgcac agtgaccgtg ccctcgctct gctgctctga ttcggctcat gcacagccgc
      901 ctgggggcgg acgaaccacc tcactccttc tggaacacgg agaacagcag atgaagcgac
      961 acttgatccc cagcctgatc gtcgggctgt ctgccctggc gctggtcgcc tgcagcacgg
     1021 gctcgaccgg atccaccgga gccaccgcgc cggcgccggc cgccacggcc gccccggcca
     1081 acacgggagg cgcactcgcc ggcaagaagg tcctttacgt cgacgcgttg ccgggcaacg
     1141 cactcctgga tggcctggcc cagggcctgg ccgccgagct caccaagcag ggcgccaagg
     1201 tggcccgggt cttccagctc aacgcccaga accagatcga cctcgccgcg gccaaccagc
     1261 ggatcaacga aggcatcgcg cagggagtcg atgccatcgt caccttcccg ttggacgcca
     1321 acgcgatccg gccgggtgtc gacgccgcca ccaaggccgg cataccgatc ttcaccttcc
     1381 aggccctcgg cggcttggac gtcaccggca aggtccgctt ccccgacgag gcgcgcggtg
     1441 aggcgaccgg caagggtgtg gccgcgttgg ccggcggcac gggcgaggcg acggtcatgt
     1501 ctggccagcc caccgacaac gtcgaggcgg ccgtccgggg agcggtcaag gggctcaccg
     1561 ggggcgggat gacgctcgtg ggagacccca acaaccagcg caacctcaag gatgacgcgc
     1621 cggaggctca gcgcatcgcg atgacgatct tccagaagaa ccccaacctg cgggtcctgg
     1681 tcgtctacaa ctcggcctcc gccacgggtg ccatcgcggc ggcccagcag gccggtgtcg
     1741 ccgacaaggt gctcattgcc acgatggcag gcgaggacac caatgtcgac caggtcaaga
     1801 agggcacctt ggcgctgacc tacgacctca acgggctgga ctacggcaag gtcatgggcc
     1861 agatggtggc caaggccctg tccgggacga agatgaccaa cgaggttgtc gacgccccca
     1921 cgggaacgat ctacaccaag gacaacgtgg gcacctacat cccgtggtcg cagcgcctga
     1981 cctacgccga catcccctcg aagttctgac accgtgaacg cgtcacctcg ccccgagttg
     2041 ccggagtccg ccgtcacggt cacttcggtg accaagcact acggcgtgac ccaggccctg
     2101 gccggggtgg acatcgcgtt ccgtcgagga gcaatcacgg ccgtcgtcgg agccaacggg
     2161 tccggcaaga ccacattgct caaggtgatc gccggcctcc tgcgcccgac ctcgggtcgg
     2221 gtgcaggggc cggacggccg ggagttgcag ggcttgacct cggcccagga gtcgggcgtc
     2281 gtcctggtgc ctcaggagcc gaccctcgcc ggtcacctct cggtctggca gaacgtcagc
     2341 ctcggccgac cggaggccgc gcgtgggccg ttcgtcaacg atcgggcggc ccgtgagctc
     2401 gccgcccggc gcatcagcgg gctccttccg cccgacgtgc tcgagcgcac gacctcgtcg
     2461 ttgagcaagt cctcccggca gttggtccag ctcgctgcgg ccatggccaa ggaacccctc
     2521 gtgctcatgc tcgacgagcc gacggcggtc ctcgacgagg acggcgtcgt ggccctgcac
     2581 gccctgatcc gcggtttcgt gggcgcgggc gggactgtgg tcatcgtgtc gcaccggctg
     2641 cgtgacgtcc tggagttggc cgacgatgtc gtcgtcctgc gcaacgggct ggtcagccat
     2701 gctggtccgg tcactgccga caccgagcac cacgtggtgc ggctgctctc cgcggaggaa
     2761 cgcgcgagtc gtccgcagca ccggcccgac cgtggacatg tcgtcctgca ggcgcgcgac
     2821 ctgcgcggct ggcggggtct gagtgttgac gacttcgccg tgcacgccgg cgagatcgtc
     2881 ggaattgccg gccagagcgg cagcggacgg tcccggctgg ccgcggtgct cgccggggcc
     2941 tacgccgctg gcggcgaggt cagcgtcgaa ggtcagaagg tccggctcgg cagcattcga
     3001 tcggcccagg ctgctggcat ctgctacatc cccgaggacc ggcaggtcaa cgcgatcctc
     3061 ggcaaccagt cggtcgcggc caacctcctc gtcggccgcg aggatcccga gttgcacaac
     3121 ggaccgttcc ggaaccgacc ggccgagaag gccgagagcg agatcctcat ggaacgcttc
     3181 gaggtccgtc cgcccgaccc ggaccgcctt gcggcactgc tctccggcgg caaccagcag
     3241 aagctcgtcg tgggtcgggc cctctcccgg cccgccaagg tcgtcgtcgc cgacgagccc
     3301 acccagggcg tcgacgcctc cgcgcgcgga gcgatccacg cggccctcgc gtcggccgcc
     3361 gctgccggcc gagcagtcgt cgccgtctgc agcgatttcg aggagttgtt cgagatctcc
     3421 gaccggatcg tcgtcctgcg cgacggccag atcgtcctcg accagccccg caagcacgtc
     3481 acccaggacg aggtgctcgc agcatccctc gggtcccacc ttcccgtcgc cgatcccggc
     3541 agccatgtat cggaggcgca tccgtcatga tcaatcccaa ccccgccgac ctcgtcggcc
     3601 agcagcctgc ccagccggcg acgtccccgc aggtttcgga cgacccgagc cgtcggcgga
     3661 tctccagcgc cacccgccag cagggcttct tcctggctgt catcgtcgcg atgttcctcg
     3721 ccgccatgcc gttcctgccg cagttccaca acggccaata cctcatgatt cagctctcgt
     3781 cctatgccgc gatcctcatc atggcgctgg gccaaacctt cgttgtcatc caggtcggct
     3841 tcgacctctc caccggtgcg atgcagggct tcatgggtgg cctggtgttc gtcttggtgg
     3901 gttggcaggt ccccgacggg ctggtcatcc tcgccgggct cggcgcggcg ctcgccgtcg
     3961 gtgtccttgt tcatgggctg ctcatcgcca aggccaagat caacttcttc atcgtcaccc
     4021 tggcgagctt cacgattctg cccagcctcc tgcgagtgct gctcaatggc tcgagcaagc
     4081 cggtcttcag cccgacgctc gatgcgatgg ccaacgggtc gatcgccggc atccgcaccc
     4141 cggtcatcat cggcgccgtg gtcttcggcg cgatgtgggt cctgctcaac cgcaccgttt
     4201 tcggacgcaa tgtgtatgcc gtgggcagca accccgaggc ggctcgcctc gcgggggtca
     4261 acgtcgaccg ggtcgcgatg atctgttacg gcatctgtgc gctgtgcgcc ggcattgccg
     4321 gtctgctcct ggtgggcagc ctgggcgcgg ccgacccgag cgcgggcttg ggcaacgagt
     4381 tcttcgccct cactgcggtg ttgctcggcg gcacccgctt ctccggaggt gcgggaacgc
     4441 tctttggcac cctgctcggg ctgatcttcc tcatcgtcct gtcgaacatc ctgctgctta
     4501 gtggcctgcc gcagttctgg atcggcgccg tccaaggtgt cgtcctcttg gtggccgtcg
     4561 gcatcgaccg cgcccgccga gacgcctgaa cctccgccga cacgcacacc caccgccctt
     4621 cctcaccagc taccccgtca ctcgcacggc atacccggtc agccggtgac cgcccacgac
     4681 cgtgacgtca tcaaccctgc ccgacggcgc ttcgcgcccg aacctaagga gtagtcgcac
     4741 catgaccctt cgcatcggca tcgacgtcgg tggcacgaac accgacgccg tcgtgctcga
     4801 cggtcggcgg gtggtcgccg cgaccaagtc gcccacgacc gaggacgtca ccatcgggat
     4861 cctcagcgcc ctcaaccagg tgctgcaagg ggtcgacacc accgaggtgt ccgcggtctt
     4921 catcggcacg actcacttca tcaacgccat cgcccaggcc cgcggtctgg agccggttgc
     4981 cgtggtgcgg ttggcgacac cgccacagag cctgttgccg atgatcgact ggcccgacca
     5041 tctgcagggc atcgtggggg acaacttcta cgtctgcccc ggcggttcgc agttcgacgg
     5101 aacgcccctc aacgccgtcg acgaggtcgc cctgcgcgac gtcgccaaga agatcgcgga
     5161 gtccggccac cggcacatcg cccttagctc gatcttctcg acggtcaacc ccgagcccga
     5221 gttgctcgcc gagcggatcc tcctcgagga gcttccgggc gtcacggtca cccgatcggc
     5281 gctcgtcggc cgcgtgggac tgttggagcg cgagaacgcg acgatcctca acgcctcgct
     5341 gctcgatctt gccggccgag tgattgacgg cctggagcac gcggtcgcta ccgcaggcat
     5401 cgacgcgccg atcctgttgt cgcagaacga cggcacggtc atgaccctcg atcgggcccg
     5461 gctcttcccg atcttcacga tcgcctccgg ccccaccaac tcgatgcgcg gcgcggccct
     5521 tctcaccggc atcgacgacg ccgttgtcat cgacgtcggt ggcaccacct gcgacgttgg
     5581 cctgctgcag cgcggcttcc cccgtgagtc gaccgtggcc atgagcttgg ccggagtccg
     5641 cagcaacttc cggatccccg acgtgctgtc cttcgcgctc ggcggcggaa cgatcatctc
     5701 cgacgacggc cagacagtcg gacccgagag cgtcggcttc cggatcaccg agaaggcgct
     5761 gctcttcggg ggcgacacgc tcaccttcag cgacatcgcc gtcgccgcgg gagccacgcc
     5821 gatgggagac cccgcccggg tggctgacgt gtcgccggac acgatcaagg ccgtcatggc
     5881 gattgtgggc gcgcgcgtcg tgcacgccgt ggagaaggcc atgctccgcg cggacgacac
     5941 tcccgtcatc gtcgtcggtg gcggcggccc gctgctcgcc gtggtgccgg ggctggagga
     6001 cctcgtcatc cccgagaacg ctggcgtggc gaacgccgtc ggtgcggcct ttgccgaggc
     6061 cgggggagag gttgaccgcg tgttcaatct cgccggcagg tctcgggtgg aggtgctcga
     6121 ccaggccaag gccgaggcga tcgccaaggc cgtcgaggcg ggcgccgacc ctgacaccgt
     6181 tcgcatcgtc gacgcggagg acgtccctct gacccacctt ggtggcgggt ccgcggtcca
     6241 tgtccgcgtc aaggccgccg gaccctttgc tcgctctgcc gccatgacct ctgctgccat
     6301 gacttcggaa ggaaactgac gtgcgcatca tcgatgaggc cgccctcaac gacatcaccc
     6361 tcggctccac ggtcctcggg gccggcggtg gcggcgaccc ttacgtcggc atgctgctgg
     6421 cgcgcgacgc catccggcgg ttcgggccgg tgccggtcgt cgacctcgat gaggtccccg
     6481 acgacgccaa cgttgcgttc attgccggca tcggcgcgcc tggtgtgctc atcgagaagc
     6541 tgccccgggc cgcggaatac gagcgagtcc tgcagaccct cgagcgtttc actggcgtca
     6601 cgtatgacta cgtctgcccg gccgaggccg gcgggctcaa cgcggtaacg ccgttcgcga
     6661 cggccgcgcc acgcggcata cctgtcattg atgccgacgg catggggcgc gccttcccgc
     6721 atttggagat ggtgaccccg acgctttacg gcggtaaggc caccccctgc gtgttcatcg
     6781 acgagcacgg gaacaccctc tatctcgaga cggagaccaa ctcgtgggcg gaggcgttcg
     6841 cccgcgcggc ggtgctggcc agtggggcga acgccgcgat ggcgctctat ccgatgaccg
     6901 gcgccgaggc caagaagtgg ctcgtccgcg gcgcgctgac catggctcag gacatcggcc
     6961 agaccatccg ccaggcccgg gttgcgaaga cgtcgcccgt ccacgccgtc ctggagtcgc
     7021 agaacggcgt catgctcttc accggcaagg tggaggaggt cgaccgtcgc aacgagcgag
     7081 ggtggacgat ggggacggcc actctgagcg gcctggatga ctacagtggc cggacgatga
     7141 ccctgcactt ccagaacgag aacctggcag ccgagcggga cggtgtcgtg atcgccacca
     7201 gccccgacct catcatggcc atggagatcg acaccggcga gccgatcccc gcggaggaga
     7261 tccgctacgg ctaccgggtt gctgtcatcg gcctgccggc cgacccgcac tggcgcaccg
     7321 aggcaggcat cgagctgtcc ggtccccgcc ggttcggcta cgacatggac taccgaccgg
     7381 ttgaggagcc tgtggcatga cccgaatcgt cctgcgtggt gggcgcgtct tcgatgggac
     7441 aggagccgac gcgttcgacg cggacgtcgt cgtcgacggc gaccggatcg tctcggtcgg
     7501 cccacccgca ggcgatggtg cctcgggtgg tgaacgggcg ggcggcggct ctggcggctc
     7561 tggcggctct ggcggctccg gtgacaccgt catcgatgtc accgggatgc tcatcgctcc
     7621 cggcctgatc gactgtcacg ttcacacggt cttcgacggg atggacctgc tgcgcgtgca
     7681 ggggcggccg ttctcgatgg agttcttcga ggcagccgtg gtcctcaagc ggctcctcga
     7741 gtgcggcgtg acgaccgtcc gcgacgccgg gggcgccgac ctcggcgtca aaacggcagt
     7801 ggagacgggg ctcatccccg gcccgcgcat ggcgatcgcc atctcggtcc tcagccagac
     7861 cggcggccac gtcgacggct ggaacgtcca cggcgacaac ctgcggctca tggtccctca
     7921 ccctggtcgc cccgactgcg tggtcgacgg cgtcgaggac atgcgcaagc gggtgcgcga
     7981 ggtgatccgg gccggtgcag acgtcatcaa ggtctgcgcc tccggcgggg tcatgtccac
     8041 tcgcgatgac cctcgccacg cgcagttctc gtatgccgag ttggccgtct gcgtcgaaga
     8101 ggccgccgcc acggggcgct ccgtaatggc gcacgcccac ggggcagccg ggatcaagca
     8161 ggctctgcgg gcaggcgtcc gctcgatcga gcacggcgtc tatatcgacg acgaatgcat
     8221 cgagttgttc ctgcagacgg gcgcctggct ggtgccgacg ctgctggccc cggtgtcgct
     8281 cgtcgaggcg atcgaggccg ggatgcgggt cacgcccgag atcgaggcca aggcgcgcgc
     8341 ggtctccgat ctgcacgtgc aggcgatcgc ccgggcgcac gcggccggag tgaagatcgc
     8401 gatgggcacc gacagcggcg tcttcgccca tggcatcaac cacaaggaac tcgcctggat
     8461 ggttcgcgcc gggctgtccc cgcagcagtc gctcagggcc gccaccgggt cggctgccga
     8521 gcttctcgag cgttccgaca tcggtggggt tgcgcccggc aagctcgcgg atctcgttgt
     8581 cgtggccggc gatccgtggg gcttcgaccg cttcgctgag aacctccagc tcgtcatgaa
     8641 gggcggccag gtcgtccgcg acgcgtgctg agggctgacg gccacgaccc agcctgcaca
     8701 cggcatacca atggtccccg ctcgctcgcc ttcccacgct caggtatgcc gcgggcgcca
     8761 gaccattggt atgtcctgtg cgcgagggtc gtcagcgctg gtcacggacg gatcatggcc
     8821 ttgagcacct cgcgggcgtc catcgcggca tacgccgccg ggatgtcctc cagatcgaag
     8881 gtgcggtcga agacgcgacc ggggttgacg gtgccgtcga gcacccccgg cagcagcgtc
     8941 tcaaggtagg cccgcaccgg ggccgggccg ccggtgaggg tgatgttggg gccgaacagg
     9001 gtgccgaaac cgactggggc ctggtcatat tggggcaccc cgacccggct gatgacaccg
     9061 ccggggcgga cgatgccgat tgcctgctcg taggcggggt agtggccgac cgcctccagc
     9121 acaatctggc tgcccccacc ggtcaggtcg cggaccgcgg cgatcccctc gtcgccgcgc
     9181 tcggcgacga ccgcggtcgc accgaactcc acgccgaggt cggtgcgggc gcggtgccgg
     9241 cccatgagga cgatctgctc ggcgccgagc aactgggccg acaggacggc catcagcccg
     9301 acggctccgt caccgatcac cgtgaccgtg ctgccgggcg ccacctggcc gcgcaccgcc
     9361 gcgtgccagc ccgtgccata gacatccgag agggtgagca gcgaggcgag cagcgactca
     9421 tcggtcgact cgtcgacgcc gggagccacg accagcgtgc cgtcggcgag cggcacccgc
     9481 accgcctggg cctgaccgcc ggccgtcccc acccgggcgt cgctccagaa gccgccgttg
     9541 ctgcacgacg tctgcagccc ggcgcggcag aactcgcacg tcccgcacga gaccgcgaac
     9601 ggggcgatga cgaagtcgcc gaccgtgagc gtgcggacct cggagccgac cgcctcgacg
     9661 acaccgatga actcgtgccc gatcgagcga ccgccgggtg ccggcggcat cgcgtggtag
     9721 gggtgcaggt cgctcccgca gacgcacgcg cgaacgacgc ggacgacggc atcggtgggg
     9781 tggacgatga ccgggtcggg ggcgtcggcg acgcggacgt ctcccgcacc gtggatgaag
     9841 gttgctctca tggtccccag ccaacatcac cgcagcgggt gccgggagtc ccgctcgttc
     9901 ctggtaccag cagggcctcc caccgacccg cccgccctgc ctacattgaa gccatgacgt
     9961 ccatcgacct gcgcgcccag atccgggagt tcctgcgcac ccggcgggcn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng acgcgatcac cgacgccccg gcgtgggtcc
    10141 gcaacggccg tcacgacatc ctcgcgatga acccgctggc tcgtgcgctt tatgcgccgg
    10201 tgctcgccga cccgcgtcgc ccggccaaca cgacccggtt catctacctg cagccggagg
    10261 cggcgcgcga gctgttcgtc gactacgacc aggtcgccaa ggatggtgct gcgatgctcc
    10321 ggctggaggc cgggcgcaac ccgcacgaca aggacctcat ccagctggtc ggcgaattgt
    10381 ccaccaggag tgagcttttc cggcaacggt gggcctcgca ggacgtgcgc taccaccggt
    10441 ccgggcgtaa gcggctgcgg caccccgttg tcggtcagct cgacctggac tacgaggcga
    10501 tggagttgtt gagcgatcct gggctggtcc tcaacatgta caccgcggcg gccggcacgc
    10561 cgacgtcgga cgccctcaag atcctcgcgt cgtgggtcgc cggacaggag caactcgtca
    10621 ccgagctcgc gccgagcaac gggtgagccg acgcccgaac ctcggctaag gtcgaccgac
    10681 gtgacggaca ccgagacccc cagcccacgt cgccgcgccg tcttcctcga cgtcgacggg
    10741 acgtatgccg tcttcggcgc cgcgcccgct ggacacgtgg ctgcggtgcg cgccgcccgg
    10801 gacgccggtc accttgtctt cctctgcacc gggcgcccga tgtcgatggt gcccgcgcac
    10861 ctgctggccg ccgggttcga cggggtggtc gccgctggtg gtgcgtatgc cgtgctcggc
    10921 gacgaggtcc tgctcgacat caggttcccc gctgacctcg ccgcgcggac cgtcgaggtc
    10981 atggacagtt acggcgtgac ctatctgttg gaggcccccg aggcggtcta cacggcgtcc
    11041 ggcaccgagg agcagttgcg ccgggcctac cgtgcccacc tcgccgatgc gccgtccgcc
    11101 acgaccgtcg tgtccgacat cctcgcgtcg gtgcgaccgg tgccggccgc cggcgcggtg
    11161 tcgttcgcca aggtctccgt cgtcgtgtcg tccgtcccgg tcgagcagtt gggcgccgag
    11221 gtcggcgaac aactcgcggt gctccccagc tcggtcccgg ccctcggcga ggaggcgggt
    11281 gagatcttcc tcgccgacgt ccacaaggcg gtcgggatgg acgccatcgt gcggcgcctg
    11341 gggctcgcgc gcgaggacgt catcggcttc ggcgacgggc tcaacgacct tgagatgctc
    11401 gagtatgccg gtgtcggcgt cgcgatcgag ggttcgcatg agcggctgct cgcggtcgcc
    11461 gaccgcaccg ccgccggtcc cgaggtggaa ggcctggtcc aggcgttcgc tgagctcggc
    11521 ctgcgctgac ctgctgcgcg tccggatggc ggcggaatcc tttacagccg tcggtagggg
    11581 cgctaccgtt gccagcggac gccctgtgtg tctacgcagt tgggcttgtg gaagtttggg
    11641 attgttactg cgcggcaggc aaaacccaag tcggaccagc agcgactctg atgggtcgcg
    11701 tgcagtcgtg gcttgggttt ttttgttccg tgaacatgcc tgcggtgatg tgagtgggcc
    11761 ccggcctggg aagcggtggc cgggccctgc tcgtcggtcg acgcgcgcct tgcgctgcgc
    11821 gtcggtcgac ccagggatat gccgcgtgag ctgtgagggg cttccggctc acgcggcata
    11881 cccttgtctg ggagcctgaa tcgagatcct ttacagcagt gggcggttgc cgctaccttt
    11941 ggtggcggac gccgtgtggc gtcccggaag tctgggattg ttactgcgcg gcaggcaaaa
    12001 cccaagtcgg gccagaggtc gactctcacg agtcgaaggc ggtcaggact tgggtttttt
    12061 tgtgcccgag gcatgcccca cgtacccgtt caggggttgg attcgaggag gaatcacgtg
    12121 gcaagtccca ctaagtacga cctgctcgcg gcggaggtca tccgcggggt cggcggcgcc
    12181 gacaacgtcg catcagtaac gcactgcgca acccgactgc ggttcgcgct caaggaccgc
    12241 gacaaggccg acaaggccgc tgtcgaagcg acgcgcggtg tcatcaccgt cgtcgagtcg
    12301 ggcggtcagt tccaggtcgt catcggcaac acggtcaaca acgtctacga ggcgattgtc
    12361 gccaagggcg acgtcaagtc cggaggtgag gtggccgcgg gaggcttcct ggccaaggcg
    12421 atcgacctcg tcaccagcat cttcaccccg ttcctgtggg tgctcgcggg caccggcctc
    12481 atcaaggcct tccttgccct cgccggcaag ctctccccgg acttcgcgac gtcttcgacc
    12541 tacgcgatcc tcttcgccgc gggcgacgcg ctcttccagt tcctgccgat gctgctggcg
    12601 gtgacggccg ccaagaagtt caaggcgaac gtctacacct cgctggccat cgcggctgcg
    12661 ctcatctact ccgcgaccat cccggtgatc gcggacgcca agggcgtgtc gatgaccctc
    12721 ggcgccttcg acgccgccag taacctcacc ttcctcggta tcccggtcgt catggtgagc
    12781 tacctgtctg ccgtcgtccc gacgatcctg gccatctacg cccaggccaa gcttgagcac
    12841 tggctggagc gcgtcatccc cgagacggtc cgcaacttcg tcgttccgct ggtcgccgtc
    12901 gccgttgttg ttccgctgac cttcctggct atcggcccgg tcgccgacct catcggcaag
    12961 ggtgtctccg gcggcgtcaa cgcgctgtgg agcctcagcc ccgtcatcgg tggcgcagtc
    13021 atgggcgctc tgtggcaggt tttcgtcatc ttcggcgtgc actggggctt cgtccccgtc
    13081 atgatccagg acctcacgac gcagggttac tcgctgctga ccggtccgct cttcgcggcc
    13141 gtcctggccc agggtgccgc cgcgaccgcc gtcatgatca agacccgcaa caaggagctc
    13201 aaggagattg ccggcccggc cgccctctcc gcggtcctcg ccggcatcac cgagcccgcc
    13261 atctacggtg tcaccctgcg tctgaagaag ccgttcatct acggctgcat cggtggcgcc
    13321 atcggtggcg gcatcgctgc tgcaggtggc tcggccgcca agggcttcgt catccccggt
    13381 ctcatcacca tgagcgcggc cgtcgacgtg ggcaacttcg ggctgcaggt cgccggcacg
    13441 gtcatcgcca tggccatcgc cttcggcttg accatggtgc tgggcttcca ggacatcccg
    13501 gccaaggccg cccttgacga tgccgctgcc accggcgagg tggcctccga ggccgtctcg
    13561 gttgctgccc cggccatcgg tgaggtcgtc tccctcgagt cggtcccgga caaggtcttc
    13621 gcctcgggtg cgctcggtgc cggcctggcg gttgttccgg ccgtcggcga ggccttcgcg
    13681 ccggtgtcgg gcacgctcat caccgcgacc tcgcacgcct acggcatcaa gaccgacaac
    13741 ggtctggagg tgctcgtcca catcggcatc gacacggtga agctgggcgg aaagcacttc
    13801 tcgcccaagg ttgttcaggg ccagcgggtc actgcagggc agacgctcgc cgagttcgac
    13861 atcgccgcga tcgagaaggc cggctacaac ccggtcgtcg tcatgatcgt gacgaacaac
    13921 ggaccgttct cggccgcggt gcccgtcgcc tccggctcca tccggatggg cgagaccgcg
    13981 ctcgatctcg tcggctgacc cggaccggcc ggggcggcat acccgtcgcc ccggccggac
    14041 cacccgcggg tcccgcgtct gacccgcccc ttgatgctgc ccctcttacg ggagggaagc
    14101 cccccacttc acgaaggatt ttgtcccgat gacgagcacc cctgagacct acttccccga
    14161 cggcttcctc tggggcggcg cgaccgccgc gaaccagatc gaaggcgcct acaccgagga
    14221 cggcaagggg ctgtccatcc aggacgtcat gcccaagggc atcatggcgg cgccgacgag
    14281 cgagcccacc cccgacaacc tcaagctcga ggccatcgac ttctatcacc ggtatgccga
    14341 tgacatcgcg ctgttcgcgg agatgggctt ctcggtgttc cggttctcga tcgcgtggag
    14401 ccggatcttc ccgctcggcg acgagaccga gcccaacgag gaggggctgg cgttctacga
    14461 ccgggtcctg gacgagttgg agcggcatgg gatcgagccg ctcgtgacga tctcccacta
    14521 cgaaacgccg ctgcacctcg cgcgcaccta cggcggctgg gtcaaccgcg acctgatcgg
    14581 cttctacgag cgctacgtgc gggtgttgtt cgaccgctac ggccaccggg tgaagtactg
    14641 gctgaccttc aacgagatca actcggtcat ccacgcgccc ttcctttcgg gcgggatccc
    14701 gacgccgcgc gaggaactca ccgagaccga cctctaccag gcgatccacc acgagctcgt
    14761 cgccagcgcg ctggccacca agatcggcca cgagaccaac cctcggatcc aggtggggtg
    14821 catggttctg gcgatgccga tctaccccct gacgtcgagc ccggacgacg ccatcgcggt
    14881 catgaacgcc gatcacggca acctcatgtt ctccgacgtg cacacccggg gcgcctaccc
    14941 ggggtatgcg ctgcggatgt tccgcgagca gggcatcgag ctcgacatca ctgatgaaga
    15001 ccgcgaattg ctcacgaaca ccgtcgattt cgtgtcgttc agctactaca tgagcatctg
    15061 cgagaccgcc gacccggccc agaagaccgc cggccagggc aacatcatgg gcggcgtccc
    15121 gaacccgcac ctgccggcgt cccagtgggg ctggcagatc gacccccagg gtcttcgtgt
    15181 ggtgctcaac cagttctggg accggtggca gaagccgctg ttcatcgtcg agaacggcct
    15241 cggggcacgc gacgagctcg tcgaggtgga cggcataccc actgttgttg acgacgcgcg
    15301 catcgcctac ctcaacgacc acctcgtgca ggttggtgag gcgatcgccg atggcgtcaa
    15361 cctcctgggc tacacgacgt gggggccgat cgacctggtg agcgcgagca cggctcagct
    15421 gtcgaagcga tacggcttca tctatgtcga ccggaacgac gacggaagtg ggacgctggc
    15481 gcgataccgc aagaagtcgt tcgcgtggta cgccgaggtg atccgcacta acggggcgag
    15541 tctggtccgc ggctgattcc tccggcactc tggaggcggg aaggaggagc agatgcaggt
    15601 cgtcaaagtt ttcaacaaca acgtcgtgct cggggccgat ggccatggcg gcgagttcgt
    15661 tctgctcggc cgcggtctcg gcttcagagt gtcaccgggg atggacatcg acgcgacccg
    15721 agtcgagcgc acgttcgtgc cgagcggggt caccaccgcc gaccggctgg ccgcgttggt
    15781 cggtgagatg ccgctggccg acatcgagct gaccgagcgg atcgttgcga tggctcggtc
    15841 gtcgttgggc tcctacgtca ccgatctcgt cgtcgtcccg atggccgacc acatcggctt
    15901 cgccctgcgt cggttggccg agggggcgcc cgagatcgag tacccgctgc ggtgggaggt
    15961 tcactacctc tacccggcgg aggtcgcggt cgcccgccag gcgctggacc tcatcgagct
    16021 cgaacgtggc gtccggctgc cggacatcga ggcggtgccg ctggccctgc acttcgtcaa
    16081 cgcgcagttc ggggccggcg acatcaacgc gacgctgcag atgaccgagt tgctgcagcg
    16141 gatcctcgcc atcatcggtg aggagtacaa cgtcgtcatc gacgaggggg ccgtcgcggt
    16201 ggcccggttc gtcacgcacc tgcgctacct cttccaccgg gagcaacagg gcaagaagct
    16261 ccagcagacg ccggcggagt tgcacgaggc gttgcgggcg gctcggcctc gcgaatacgc
    16321 ctcggccggg cggatcggtg agctcctgcg cgcccggttc ggttgggaga tcaccaacga
    16381 cgagatcctc tacctcgcgt tgcacgtcag ccgcctgatc gacgacggct ccaccccnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag cagatcctga ccgcggtcgg
    16561 cggcccggac aacgtcgcga gtctcacccg gtgttgggcg aggctgcgtt tcgagttgca
    16621 cgacgctgcc gtggtcgacg aggccgccat cggcgcgctg cccgaggtcg ctctggtcgt
    16681 cacacagcat ggccagttcc aggtcgccat gcgcaccggg ctgttggagg tcttcgacga
    16741 gttggtggcg cttctggagc gttgaccgac ctgcgactcg ttgtccctgt ccgcctcgac
    16801 tgctccctca atcgatcgtc ttgtcacgtt tcgaacgcca atccacgtcc caaggagaac
    16861 ccatggccac ccgttccgtc atcatcgcgt ccagcgtcgg cctgcacgcc cgccccgcgt
    16921 ccatcttcgt ccaggcagcc aacgccaccg gcctcgacgt cgagatcggc cgtcccggtg
    16981 aggagcccgt cgacgccacg agcatcctcg gggtcatgtc cctgggtgcc ggccacggcg
    17041 acgaggtcgt gcttaccgcc gagggcgacg gcgccgacgc ggccctcgac gagttggtcg
    17101 ccttgctctc gcgcgacctc gacgccgagt aggcgctgcg caccacccgc agaggtatgc
    17161 cgcccgcgcc aggtgcgcgg gcggcatacc tttgcgtcgt tggccccttc gcgaggaagc
    17221 gtgttacggc accgggtgct cgccgactcg ccaccgggac aggtgtgcgg cggcctcctg
    17281 gtaggtcgag gcgccgctgg ccatggcgat caccaggtcg taggcctcat cgtcaggagc
    17341 gtcgagggtg atgtcgttca agccgtagaa cacgacgatg gcaagccagc cgagtcgctt
    17401 gttcccgtcg atcaaggtgt ggttgcgcac cagggactcc aacaaggcgg cggccttctc
    17461 gtcgatgttc gggtaggcat cgacaccgaa cacgctcgcc tggggtcggt gaacggctga
    17521 gtcgagcaga ccgacatcgc ggactggtcc cacgcccaga tcgtcgatca gcgtcagaac
    17581 atcctcgaga gtgaggtatt cgatcactgg ccgaggcggt cgagcagatc cgcatatcga
    17641 tgccgtgccg aggccgacag tccagccatc ttgtcgccgc gggcccgacg agaggctgcc
    17701 tcgtggatcg cgcgcacggt cgcctcctgc ttgctcacgc cgtcggcctt ggccaacatg
    17761 gtgagcgcct cctcatcctc gggagtcaac cgcagagtca tggccatgag gtcatggtat
    17821 caagttggta tcgcaaccac ggagtcaaag cccgccgcac gtatgccgcg tggccggatg
    17881 cgcgcacggc gataggatcg cttcagttcg ttgaacaaaa caacgtgagg tgggcagtgg
    17941 ggccgacgtc gtcgaagaag tccggtgctg ccgaccgggt tgcggcggtg ctgcgggagc
    18001 agatcgtcaa aggcttgttc cccccgggtt tgcggctgtc cgaggaagcg ctagggagcg
    18061 atctgggggt ggcgcgcaac accctgcgtg aggcgttccg gatgctcagc caggagcgcc
    18121 tcgtcgttca ccggctcaac cggggcgtgt tcgtccgcga gctcaccgtg gcggagatcg
    18181 aagacctcta ccggttgcgc tcggctatcg agatcgcggc gttgtcgcag gggggcctcg
    18241 tcgagcccga actcgggcca ttgcgtgcgg ccctcgccga ggccgatgag gctgccctcc
    18301 gcgaggattg gaaggcggtc gggacggctg gcctcgtctt ccattcgcga ctggtccaac
    18361 tcgcaggcag cgagcggctg tctgcggtga tggacggact catggccgag ctgcgcctgg
    18421 ccttccacat ggcggcgaac gtcaacgagt tgcacacgcg ctatgccctg cgcaacaaga
    18481 tggtcttcgc gttgctcgag gagggcgaca tcacgggagc ggcggtcgcg ttgcgggact
    18541 acctgacgga ttcggaggcc gacatcctcc aggcctactc cggagttcgg gcctagtagc
    18601 cagattgttg aacaaaactt gacgcggctg cgagcctctg ggcagactgg caccgccccg
    18661 gcttgacccc gacagccgca gggtggagcg cgatcggagc gtcatgacca cagcaaccaa
    18721 gccgccgacc cccagcccgc acgtgccggt cggcctggac tggcacctag cgatcgcgca
    18781 agagctcatc gcgcgagggg tgagcgtcgc ggcatacgtc ccggactccc ggctcaacgg
    18841 ggtgttggcc gccctcgggg agcgggacat ccggctgcgc aacctcgcgc gcgaggagga
    18901 gtgcgtcgcg tatgccgccg ggcaacggat cgccgaggcc cgtcccgtcg tcctcatgca
    18961 gagctccggc ctcgggaatg ccttgaacgc cctcgggtcg ctggccgtgc cctaccgcct
    19021 aggcatcccg ctcatcatct cgatgcgcgg caccctgggc gagaagaacc cctcgcaggt
    19081 gccgatgggc cgggcgacaa cggcgtggct cggcgccctc gggatccaga gtttctcgct
    19141 ccggcaggca tccgaggtcg gcccggtgat ctccggcgtg ctcgatctcg ccttcgacgc
    19201 cggcgagacg gccgccatgc tcctcgagcc cgagttggga ggtggacggg catgaacgtc
    19261 aacgatgtga cccgcacgat catgcagtgc cggcccgacg cgatcctcgt gtcctccctc
    19321 ggcaccgcga cctcctcggt ccgcctggtg acctccgacg gcccacactt ctacttcggc
    19381 gccgccatgg gctcggcact ggcgggcgcc ctcgggctag ccgaggccgt gcctgaccgc
    19441 caggtcgtcg cggtcctggg agacggggag ttcctcatgg gggccagcgc gctgtggagc
    19501 gtcgcggcat accggccgag caacctgacc gtcgtggtcc tggccgatgg ggcctactcc
    19561 atcaccggag gacagccgat cccggcgcct ctgcacgtcg ccgcggtggc caacgccctg
    19621 gccgggacgc gtggggttgc ggtgagttcg acgaccgaac tcgccgcaac cctgcgctcc
    19681 gaacccacct cgccggacgc gcccggcacc cctctcgtca tcgaggcgac gatcagtgag
    19741 cgcacctggc ccggtccgtc gcccttcatc gaccccgctg ccaccgtgcg caacctgcgc
    19801 gcgtccctgg ctcaggcacc cgccccagcg tgaggacttc gcgggactga ggtatgccgt
    19861 tcggcctgag gcggtcgcgc gcgacgttta ggtggccgcc ccatccgcgg gggtatgccg
    19921 ccctcccgca cccatgacca gcccgctcgc caccacgacg agggccatgc cgaccaattg
    19981 ggttggggtg agccgctgtc cgagcacgat gaggccggcg agggcggcga cggcgggctc
    20041 gagggagagc aggatgccga agactcgctg gctgagttga cgcagggcga gcaactccag
    20101 cgaatagggc aggacactgg agaggatcgc gacggcggcg cctttgacga ggacgtcgcc
    20161 agtccacagc ggcacggacc agattccgaa gggcagcacg agggcgagcg cgacgatcat
    20221 cgccagggcc agcccgttga gtccggggaa cgcggcgccg gtccgctggg acagcacgat
    20281 gtagccggcc cagaaggtgc cggccagcag ggccaggccg atcccgatga ggtccaggtc
    20341 gccccagggc acggtgaggg cttgggagat gagcaggatg ccggctgcgg cggccgccac
    20401 ggcgagcaga tcccggaccc ggcgggactg cacagcggcc aaggtgagcg gcccgatgaa
    20461 ctcgatcgtc accgccaccc cgatgtgcag gcggtcgagg gaggcgtaga acgaccagtt
    20521 catcgcgccg aggacgaggc cgaagagcac gacggtgcgc caggccgagg ctgagtgccc
    20581 gcgtagggtc gggcgcgcga ctgccagcag gacgactgcg ccgatgccca gccggagcac
    20641 gaccgacccg gcggcgccga tgacgggcac gagcgtggcg gcaacggccc cgccgaactg
    20701 caccgagacc accgcggcca tgaccaggag cagcgatccg ccggaggtct gccgcgcggg
    20761 catggcaggg ctccttcgtc cgggccgtcg aggctaactg tcggggccaa cctcgggatc
    20821 aacaggtcaa ccgctcgacc ctatccccgt ccgccccggt tgggacgccg ccgaccctgg
    20881 ctccggtggg cgattcatgg ttatcatatg cacggaccta gggagacgac cgatgacgat
    20941 cgagcccgcc gggcagcact tcccccactt gttcgctccc atcaccgtgg gaacgatgcg
    21001 gttgcgcaac cgcgtcatgc tgcccccgca cgcctccgcg atcggcaata tctacggcac
    21061 cgacgacgag gcagcgcgca acatcgccta cttcgaggag cgcgcccgct cgggcgtcgc
    21121 ctggatcgcc tcgctgtcca cccacatccg caaccacctc atccccggct tcgacccgac
    21181 cggggtgggc gcggccacga ccggcttctt ccggctgcct ttcttcgtcg agcgggtgca
    21241 ggccttctcc gatgcgtgcc acgcgctcgg cgcgacggtc agcgtgcagt gcgtccacca
    21301 agggggtatg ccgcacgccc cctccgcgcg cctgagcgcc ccggtcatca acctggtccc
    21361 gcacgtcatg acccacgccg acatcgccga gttcgtcagg gagtatgccg agtcggctcg
    21421 gctgtcgatg ctcggccgcg ccgacggatt cgagttgcac ctcaaccacg acgacctgca
    21481 cgagtggttc ctgtcgccgt atacgaacta ccgaaccgac cgatacggcg gctcgctcga
    21541 gaaccggtgt cgcttcgcga ccgaggtctt gcaggcggtc cgcgacgtcg tcggcacgtc
    21601 gatgaccgtc ggcatccggc tcaacatccg ccaggaggcg cccgagggct tcggggtcgc
//
LOCUS       DAORPW010000029        21042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_426_length_21042_cov_750.835660, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000029 DAORPW010000000
VERSION     DAORPW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 21042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21042
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_426_length_21042_cov_750.835660"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..984)
                     /locus_tag="PLB86_04165"
     CDS             complement(112..984)
                     /locus_tag="PLB86_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRC64363.1"
                     /translation="MRVSRYAAPPGPWTPGRPAVVGHRGARGLIPENTVASMRAAIDV
                     GVPAIELDVLLTADDQVVVWHDPVLHPAKCRPTGADYLGARVDELTLTQLRTVDVGSL
                     TLAGFPRQRAVPGERIPTLAEVFAATTGAPIWWVIEVKIDVTDPRDRAARHTLLNGVL
                     EAVTDAGVRDRVFIHSFDWAVLQRARVLDPHLPVSALAVVGETFAPRSKWLGTVQWDD
                     HPDDLAGAAAELGASVVSPDFHSCTAELVEQAHARGMAVLPWTVNDPVIFGDLADIGA
                     DGVVTDLPDEMLAL"
     gene            complement(1011..2459)
                     /locus_tag="PLB86_04170"
     CDS             complement(1011..2459)
                     /locus_tag="PLB86_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRC64364.1"
                     /translation="MATATALRQPVVIQGGMGVAVSDWRLARAVSVTGQLGVVSGTAL
                     DVLLTRRLQSGDPGGHVRRALAAFPRPEVSRWILDSYFVEGGIGAEGRFAQVPRHTLS
                     SSVRLQELTVAAGFAEVFLAKEGHDGLVGINFLRKIELPLPATLYGALLAGVDFVLVG
                     AGSPADIPGMVRHLASHQPVTVSVKVMGARSTDNVGDLVFDPAVALPQRPATLPMPRV
                     LAIVASTDLARGLAENPETRPDGFVVEGAAAGGHNAPPRGPRRVDEIGQPIYDERDVV
                     DVADILSLGLPVWLAGGYGTPDKVAEALALGAAGVQVGTLFAYSDESGFDADVKAQMR
                     EKAVAGELQVRADWRVSPTGFPFRVADIPGTLTDVTVRAARKPVCDLGVLRSAYLKAD
                     GEVDYRCPAEPLAQYVRKGGREMNTEGRLCLCNALFASAGFPQRRPGGASEAPLVTSG
                     TDLEPVVHLIGASDGPGYGAADAVAHLLSRVS"
     regulatory      2612..2755
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2842..3642
                     /locus_tag="PLB86_04175"
     CDS             2842..3642
                     /locus_tag="PLB86_04175"
                     /inference="COORDINATES: protein motif:HMM:NF040672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO2322 family protein"
                     /protein_id="HRC64365.1"
                     /translation="MLSVTPSIAARRSASRRAFAVLAAVMVAFATLVSTAPQATAANY
                     LYWSYWQQTAGKWAFSSVGADKATPSDGAVEGWRWAIDDGNGSRPPRVLPTFEQLCAS
                     TPAEAGKKRVGLVVDFGRDVDGDGKVTPPSLVATCALVPTAATGADLLAKAGGVRTDK
                     GLICAIGGYPATGCAAQLETLTEAQKAPDTALTLPTAAPTTAPTATTPVATTTTAATP
                     PATATAAVVATSTTAAAGGTSPLLWVLLFVAAAGIAYAVARRRRPSGV"
     gene            3658..4791
                     /locus_tag="PLB86_04180"
     CDS             3658..4791
                     /locus_tag="PLB86_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRC64366.1"
                     /translation="MARPTPAATPRMLHPVAWWVWGVGLATAAAHTTNPWLLLLVIAV
                     AGWVVLERREVGALDSFRPFLIIGLFAIGLRVALTILLGNGVPGQVVLVELPQVRLPD
                     WFAGLRLGGPVTLESVVAAGIDGLRLATTLACLGAANGLASPRRLLRYAPATLYDIGT
                     AIVVALTFAPALVDLAGRVRSARRLRGHSGTGVRELARLGVPVLEGALDRSLDLAASM
                     ESRGYGRTAVRGRRERIVGSALALSGACGSVAGLFGVMSASSGGWFGLPMLVSGLALL
                     ATSLVLGSRRDARTAYRRDPWRAPEWATCALGLLPAVLLVHAEADAWPGITITQVPLD
                     WPPLPALPTLAILAAVLVAWVTPVPPRLARARATTLPARLETA"
     gene            4788..6410
                     /locus_tag="PLB86_04185"
     CDS             4788..6410
                     /locus_tag="PLB86_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC64367.1"
                     /translation="MIELRGVGVTYAGASRPSLRDVDLRIEEGELVLIVGPTGSGKST
                     LLRTLNGLVPHFSGGTLTGSVVVGGRDTRAHRPRDLADLVGFVVQDPTSAFVTDTVED
                     EIAYGMETLGVEPTAMRRRLEETLDIMGLAELRDRSLRELSGGQQQRVAIAAVLAAGP
                     RVLVLDEPTSALDPVAAEEVLSALHRLVHDLGYTVVLAEHRLERVVQHADRIVLVRDG
                     VVSAPLDPAEAMALSPVHPPIVELGRRMGWSPLPLTIRDARRRAGSLRARLAALDGAP
                     AAHAPVKDAASRSLVSVNRLVVRRGIVEALSKVTLGVSAGSVTAVMGRNGAGKSTLLA
                     AMAGQLAATSGEVSVAGSSPRRLAPHDRVRVVGLVPQDSNLLLYADSVAAECRAADRD
                     FAAADGTTRALLARIAGEVDGDQHPRDLSEGQRLALALAIILAGRPQVVLLDEPTRGL
                     DYPAKRRLVGILRDLAAEGHAVIVATHDVELVAEVAQRVIVLAEGEVVVDGPIHEVLT
                     GSPAFAPQVAKILHPLPLLTVDDVSAALVPAS"
     gene            6407..7186
                     /locus_tag="PLB86_04190"
     CDS             6407..7186
                     /locus_tag="PLB86_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRC64368.1"
                     /translation="MRFRPAAAAALALTTVVGLAAYLWPFFAAPGFSTSYGQDAPWLF
                     AALLGLMAFVVLAEVTANGLDAKTVAVLGVLAAAGGALRVLSAGTAGLEPIFVIFVLG
                     GRVLGRSMGFLMGSLAMLAGAFLTAGVGPWLPFQMLGAGWVALGAAMLPRATGRVELA
                     MLAGYGAFAGLAYGVLLNLWFWPFLAPASAPTGAGFVPGDSAAANFAHYAVFYVATSL
                     GWDVPRAVLTAVLILVAGRSILATLRRAVRRAAFGEVVRFD"
     gene            complement(7183..8283)
                     /gene="gcvT"
                     /locus_tag="PLB86_04195"
     CDS             complement(7183..8283)
                     /gene="gcvT"
                     /locus_tag="PLB86_04195"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRC64369.1"
                     /translation="MADLAHSPLHDRHVALGAKLADFGGWEMPIEYPGGGVVAEHTAV
                     RERVGVFDVSHLGKATVSGPGAADFVNACFTNDLRRVGPGQAQYTLCCDATGGVVDDL
                     IQYLRADDDVFLIPNAANTAEVCRRLQAAAPEGIEVVDRHRDYAVIAVQGPRSDEVLD
                     ALGLPTGHDYMQFREADWQGRPVIVCRTGYTGERGYELVPRWDDAADLWDAIIEAMLP
                     WGGMPCGLGARDTLRTEMGYPLHGQDLSLDITPVMARSGWAVGWAKPAFWGREALLAE
                     KAAPTSRISRGLLVTGRGIPRAHCPVTLDGEVIGEVTSGTFSPTRKQGIALALLDRSV
                     PLDAEVLIDVRGRGVEATVMNPPFVQVQTRED"
     gene            complement(8354..8554)
                     /locus_tag="PLB86_04200"
     CDS             complement(8354..8554)
                     /locus_tag="PLB86_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HRC64370.1"
                     /translation="MSKNAKSGKAPKAAGPSDEMKAKFREALERKQAHAGRESAGEGE
                     GKVHDAHGPAAAQRMFRRKSGG"
     gene            complement(8572..8949)
                     /locus_tag="PLB86_04205"
     CDS             complement(8572..8949)
                     /locus_tag="PLB86_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64371.1"
                     /translation="MGLFDRWRRSSLPDLGRSDGPAMRVDLATVRGHFAQFVQTRRGV
                     EAFLEPATNVSTQSVVLVATDGEWTRRAVGSRAAAYEMAAALGIPIYDVLLTGYPSRM
                     REWSSRQRRDKRGDTRPGSRSDD"
     gene            8973..10106
                     /locus_tag="PLB86_04210"
     CDS             8973..10106
                     /locus_tag="PLB86_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64372.1"
                     /translation="MSTRGTSTGRWAGVGVAGVIILSLASIGAHALLIPGFIRAADAS
                     SRSTGALSTPTTVQSGQGGTGSTSSTSSPAATDLAPTAVPAMPAYDRACLRRDNPATG
                     AKIPANDPFGDDIADLGDKAELQRLYAVQSGALVPLDGLGAPRPCDEQLWSLVKATAP
                     TLLGHIDELLVFDADPDPATGEFIIEGESAPKETAPDTFDDDHWRVSFAPNGLDRGEL
                     AWLVAHELAHVASLNKDQMLSGVGADVCATWYTGTGCLLADSFLRRYLSNTWDDALWD
                     DWDKADGKSTEKARRAAYQDFYDSHSDSFITEYAAWHPLEDFAESFAMWCTYDEDEPD
                     RANLPTAERTDSGNKVAWFDAARRDLLPAFGPGCTMLRQFAVS"
     assembly_gap    10149..10248
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10249..11304
                     /locus_tag="PLB86_04215"
     CDS             <10249..11304
                     /locus_tag="PLB86_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRC64373.1"
                     /translation="ATIDYVDGFGEVTSPTTVRVGDRTLTGRRLVLATGSRARSLPGL
                     EIGGRVLTSDQALGLTQVPTSVIVLGGGVIGVEFASVFRSFGASVTIIEALPRLVPAE
                     EESISKALERAFRKRGIVSRTNARFASVAQDDASVTVSLEDGSTLSADLLLVAVGREP
                     VSDGMGFQEAGVLTDRGFVPTDERLRTNVPGVYAVGDLVPGLQLAHRGFAHGVFVAED
                     IASLNPAPVLDRNIPKVTYCDPEIASVGLTEAAARAEHGEVRAYEYNLGGNGKSQILG
                     TSGFVSVVRVVDGPVVGIHMIGARMGEQIGEAQLVVGWDAMPEDVAPLVHAHPTQNEA
                     LGEAHLALAGKPLHAHA"
     gene            11374..>11657
                     /locus_tag="PLB86_04220"
     CDS             11374..>11657
                     /locus_tag="PLB86_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HRC64374.1"
                     /translation="MSNRVTMPALGESVTEGTVTRWLKKVGDPIAIDEPLVEVSTDKV
                     DTEIPSPFAGIVEAILVAEDEVAAVGADLAVIGDGSGAAAAPAAPAVEAAP"
     assembly_gap    11658..11757
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11758..12615
                     /gene="sucB"
                     /locus_tag="PLB86_04225"
     CDS             <11758..12615
                     /gene="sucB"
                     /locus_tag="PLB86_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HRC64375.1"
                     /translation="VAPAPVAPAPVAAAPAAPAPVAAAPAAAAPAASAAPASARPSLQ
                     PSSKRGTTEKMSRLRKVIASRMVESLQVSAQLTTVVEVDLTRVARLRARSKAAFQARE
                     GTSLSYLPFLALAAVEALKVHPMVNASINGDEVIYHGQEHLSIAVDTDRGLIVPVVKN
                     AGDLNIAGLARGIADVANRTRTNKITPDDLTGGTFTITNTGSRGALFDTPIINQPQVA
                     ILGTGALVKRPVVVSDAEGGETIAVRSMMYLALSYDHRIVDGADAARFLETMKHRLEE
                     GNFEPDLGL"
     gene            12626..13531
                     /locus_tag="PLB86_04230"
     CDS             12626..13531
                     /locus_tag="PLB86_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRC64376.1"
                     /translation="MSGRRVAVTGASGFIGGALTAYLAGRGDEVVRLVRHQPTQPSER
                     RWDPVRGGLDPRAIDDVDAIVHLAAAGVGDHRWTPAYKELVLRSRVDGTAAVATAIAQ
                     TGRPIRFVCGSAIGYYGDRGDEILTELSGPGTGFLAEVARAWEGSAEPAIAAGAPVAF
                     ARHGLVMGPGGGAFARLLRLARWGLFGPLGSGRQYWSWITLADSVRALSHLIDRPDLT
                     GPVNIVGPDPRPQRVVASAIGHALHRPAMLPAPSVALRVALGEFADDILGSQRVMPFA
                     LTGSGFTFEHSMLRPAAAWLVRESS"
     gene            complement(13577..15112)
                     /locus_tag="PLB86_04235"
     CDS             complement(13577..15112)
                     /locus_tag="PLB86_04235"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRC64377.1"
                     /translation="MSAAKTIPPSVPGLVLGQVIGRGATSTVWSAVRVDDGRAVAVKI
                     TSPDRLHVGQLMELAARETAILATVEHDHLVRLHEAHALPDGSVAVVLDLAPGGSLAD
                     LVAARGRLDGGEVATICTPLAGALAALHAAGVVHGDLSPGNVLFTADGKPLLSDFGAA
                     RLVGEGHPPLVAGTSGFVAPEVVAGDVPGEAADVFGLGALAWFALTGRSWSVRSWATP
                     LGGADTSVPGPVTVDEAVAVLGAGFGPVVAAMLSQDPSGRPEAQEAAVLAYQAASPVA
                     VRLVGSAAGADPDRVLTQRLRGAAGRVTPGVGAGPGRSQAVRRGQAARRGRAGGRVPG
                     PRVGRKARLGLGAALAIVLAAVVVGVFLRLSPAAAAEGSAGEVTRVLGSAAAPAGEAR
                     AVLATLVGRRAQALTARDLRALAAADGQGSTLLAADEAIIAELVQAEQRYVGLTFTVT
                     VAEWVSVDAGSARIRAVVERSGYRVQGPGDREQSIGADSGRSLLYSLVRVGEEWRIST
                     VTG"
     gene            complement(15223..16260)
                     /locus_tag="PLB86_04240"
     CDS             complement(15223..16260)
                     /locus_tag="PLB86_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC64378.1"
                     /translation="MTRVGLVGYGSSGAGFHAPLLREAGLDLVAVVSGNPERADQARA
                     ENPGVVVVPDLAALVAVPDLDVVVVASPSGRHVEHAEALLEAGIAVVVDKPIATDAHQ
                     AQGLVDLAAGRGVPLTVFQNRRYDPEFATIRDLLHRGVLGELRRTEMRWDRWRPVPKD
                     RWRERATAAEGGGLLLDLHTHLLDQVVLMHGPVATVYAELAAWTVTAEDDTFVALRHT
                     DGYSTHLSASSVAGAPGPRARITGSAGTFLLGSAGGEPTAFHDADGGPGQHGWLVRGD
                     EREAVLARSSDPADFYREVAAALASPDPQESMPVDPRDAVHVLAVIDAARISAADGRV
                     VDVITPGEDPH"
     gene            16332..17030
                     /gene="lipB"
                     /locus_tag="PLB86_04245"
     CDS             16332..17030
                     /gene="lipB"
                     /locus_tag="PLB86_04245"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRC64379.1"
                     /translation="MPSRRVKVGLMRVVHLGFDPDFVDYEWAWAHQREVHAKVVSGEE
                     PDTVLLLEHQPVYTAGKRTEAHERPNDGTPVVDVDRGGKITWHGPGQVTGYPIVRLPA
                     PIDVVAYVRLLEEMMIRVCADFGVSAGRVDGQSGTWILADPADPRRRPNRKIGAIGIR
                     VSRQVAMHGFAFNANPSLAWADVIIPCGIPDAGVTSLSAEVGRDVPVREVLPHIEKHL
                     ADVLGTLAPNRRAI"
     gene            17106..18143
                     /gene="lipA"
                     /locus_tag="PLB86_04250"
     CDS             17106..18143
                     /gene="lipA"
                     /locus_tag="PLB86_04250"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRC64380.1"
                     /translation="MTIAPEGRRMLRVEARNAQTPIERKPEWIRTTAKMGPAFTELHS
                     MVKGEGLHTVCQEAACPNIFECWEDREATFLIGGDTCTRRCDFCDIATGRPQPLDPDE
                     PRRVAESVRRMGLRYSTVTGVARDDQPDGAAGLYAATIRAIHELNPNTGVEILPPDFG
                     AKPELVGQVFDARPEVFAHNLETVPRIFKRIRPAFTYDTSLRVLSMAREAGLVTKSNL
                     ILGMGEEAQEIEAAIVDLHDAGCDILTITQYLRPSAKHHPIDRWVKPEEFLHWSSVAT
                     EHGFKGVMAGPLVRSSYRAGRLWAQAMARWGRPVPEHLAHLAEAAGAPARQEAAAVVA
                     AFAERSGAPVS"
     gene            18149..>18867
                     /locus_tag="PLB86_04255"
     CDS             18149..>18867
                     /locus_tag="PLB86_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HRC64381.1"
                     /translation="MAKDSSSTAAPKKPGRIAQLRTVLQQSKALDPKIVWWMAGAFVL
                     TLAVIVGIGWLVNWPVYAAILGLPLAALAATIVMSRRAEGAAYAKLEGQPGAAGAALS
                     SLRGGWFTSSEPVAVEGARSGSMADAALVYRAVGRPGVVLVAEGPEARAQRLLLAEKK
                     RTERVAPGVPVTVYRVGSGKEEGVVEIRKLTSKVQRMRATLSKDDVLKVNQRLRALGG
                     VKLPVPQGIDPARARPDRKGMR"
     assembly_gap    18868..18967
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18968..19653)
                     /locus_tag="PLB86_04260"
     CDS             complement(<18968..19653)
                     /locus_tag="PLB86_04260"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRC64382.1"
                     /translation="MIGSPADLADVLRAAAKGEFPAADGGWERASLWLRGVEAVVAMT
                     GHAYLAVGQDLADVELDAYGVDGYGGAHHPRVAELIRGSGWADALDVLLVRPPDAPRP
                     TATGPDLVSRDDLAGHPRVAVAQHVRSGIRVLGMPSRRVRSVVTVASGLGGLPEIGLQ
                     TEGPTSGRELLAAALRWVDDELGHSDPLVAAVAPGNARSLRLFLHVGFVPVGSVQLFR
                     PERHPWQDDTA"
     gene            complement(19650..20114)
                     /locus_tag="PLB86_04265"
     CDS             complement(19650..20114)
                     /locus_tag="PLB86_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRC64383.1"
                     /translation="MVDRRDVASWLEGPRARTQAPGEYPGQDLGMPPSGPGSIARFGR
                     RLVAICIDWALCQLIAVFVFRAELGQGGAGSFAPLAVFAVENLLLLPTLGATVGQRLL
                     GIVIMTVDRRRISVVQATLRTLLLCLAVPALIWDRDSRGLHDKAAATVLVRR"
     gene            20300..>21042
                     /locus_tag="PLB86_04270"
     CDS             20300..>21042
                     /locus_tag="PLB86_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HRC64384.1"
                     /translation="MFTSADEVLAYIKAENVKFVDIRFCDLPGVMQHFNVPAHTLDED
                     FFTVGQAFDGSSIRGFQAIHESDMKLLPDVATAYLDPFRVEKTLVINFSIVDPFTDEP
                     YSRDPRNIAAKAEAYLKSTGIADTAFFGAEAEFFVFDDVRFKTSANESYYYVDSKAAV
                     WNTGKVEEGGNLGYKPRMKGGYFPVSPVDHFADLRDEMCLRLAEVGMEVERSHHEVAS
                     GGQQEINYRFNTLLHSGDDMMKFKYVIKNT"
BASE COUNT         3036 a         7512 c         7122 g         3072 t
ORIGIN      
        1 gctgccccga tgatgaagcc gagcgccagg tcgagcatgt tgccgcccat ggcgaaggcc
       61 ttgaactctt tccacgcgtt cttcatggcc gacattgtcc ggcggcgccg gtcagagcgc
      121 cagcatctcg tccggcaggt cggtcaccac cccgtcggcg ccgatgtctg cgaggtctcc
      181 gaagatgacc gggtcgttga cggtccaggg cagcacggcc atccctcggg cgtgggcttg
      241 ctcgaccaac tcggctgtgc aggaatgaaa gtcgggcgac acgacggacg ctccgagctc
      301 cgctgcggcc cccgcgaggt cgtcgggatg gtcatcccac tgcaccgtcc ccagccactt
      361 gctcctcggc gcgaaggtct cgcccacgac cgccagcgcc gaaaccggca ggtgcgggtc
      421 aaggacgcgg gcccgctgca ggacggccca gtcaaaggaa tggatgaaga cccggtcgcg
      481 cacccccgcg tccgtcaccg cctcgagcac accgttgagg agggtatgcc gggcggcccg
      541 gtcgcgcggg tcggtcacgt cgatcttgac ctcgatgacc caccagatgg gtgcaccggt
      601 ggtggcggcg aacacctcgg cgagcgtggg gatccgctcc cccggcaccg cccgttgccg
      661 gggaaagccc gcaagcgtca acgagccgac gtcgacggtg cgcagttggg tcagggtgag
      721 ttcgtccacc cgggccccga ggtagtcggc gccggtcggc cggcacttgg cgggatgcag
      781 caccgggtcg tgccacacga cgacctggtc gtcggcggtg agcaggacgt ccaactcgat
      841 ggccggaacg ccgacgtcga tggccgcacg catgctggcc acggtgttct cagggatcag
      901 ccctcgggca ccgcgatgcc cgacgacggc cgggcggcct ggtgtccacg gtccgggcgg
      961 agcggcatac ctgctgacgc gcatgccgca acgctaaccg gcagaaccgc tcagctgacg
     1021 cgcgacagca ggtgcgcgac ggcgtcggcc gcaccgtagc cgggaccgtc gctcgcaccg
     1081 atgaggtgga cgaccggctc gaggtcggtg ccggaggtga ccaacggggc ttcggacgcg
     1141 ccaccggggc ggcgctgcgg gaacccggcg ctggcgaaca gcgcgttgca caggcacagc
     1201 cggccctcgg tgttcatctc acgcccgccc ttgcggacgt actgcgcgag cggctcggcc
     1261 gggcagcggt agtcgacctc gccgtcggcc ttcaagtagg ccgagcgcag gacgcccagg
     1321 tcacacaccg gcttgcgggc ggcccggacg gtgacgtcgg tgagcgtgcc ggggatgtcg
     1381 gcgacgcgga acgggaagcc cgtcggcgac acccgccagt cggcccggac ctgcaactcg
     1441 ccagcgacgg ccttctcgcg catctgggcc ttgacgtcgg cgtcgaagcc cgactcgtcg
     1501 ctgtaggcga acagggtgcc gacctggacc ccggcggcgc cgagcgcgag ggcttcggcg
     1561 accttgtcgg gggtgccgta gccaccggcc agccacaccg gcagaccaag ggagaggatg
     1621 tcggcgacat cgacgacgtc gcgctcgtcg tagatcggct gaccgatctc atcgacgcgc
     1681 cgcggcccgc ggggcggggc gttgtgtcca ccggcggccg cgccctcgac gacgaaaccg
     1741 tcggggcggg tctcggggtt ctcggccagg ccgcgggcca ggtcggtgga ggcgacgatg
     1801 gcgagcacgc ggggcatcgg caaggtggct ggacgctgag gcagcgcaac ggcggggtcg
     1861 aagaccaagt cgccgacgtt gtcggtcgag cgagcgccca tgaccttcac gctgacggtg
     1921 accggctggt ggctggccag gtggcgcacc atgcctggga tgtcggcggg gctgcctgcg
     1981 ccgacaagga cgaagtcgac cccggcgagc agggcgccgt agagcgttgc gggcagcggg
     2041 agttcgatct tgcgcaggaa gttgattccg acgagcccgt cgtgtccctc cttggcgagg
     2101 aacacctccg cgaagcctgc ggcgaccgtc agctcctgca ggcggaccga ggaggacaag
     2161 gtatgccgcg gcacctgggc gaagcgcccc tcggcaccga tgccgccctc gacgaagtac
     2221 gagtcgagga tccatcggga cacctcgggc cgggggaagg ccgccagggc tcggcgcacg
     2281 tggccgcccg ggtcaccact ttgcaagcgg cgggtcagca ggacgtccag cgcggtccca
     2341 gagacgacgc cgagctggcc ggtcacggac acggcacgag cgagccgcca gtccgacacc
     2401 gcgacaccca tccctccctg gatgacgacg ggctgacgga gggcggttgc ggtggccatg
     2461 acagggcctc tcggagtctc gaacttacga ggacgtaact tacgttaccg taacctacgg
     2521 tgccgtaaca acgacacgcc ggtgtcaatt gcgcaaacgt gtcgcacggc ataccgacag
     2581 ccctccccct ggctttgaca gcgcgcccct gcccgtggga acgtcacgtc accgagaccc
     2641 gtccacgggg gaagccggtg caaaaccggc gctgtcccgc aaccgtgacc tctcgcccga
     2701 cttcggccgg tgcgcagggg aagtcggaac gcctgtggcg gtgtcggctc caacacatcg
     2761 tccgtcgtgg aaagcggacc ggagcgaagc gtcgggcgac aagcccgggg cctcccaccg
     2821 gccccgagga ggtccccacc catgctgtct gtcaccccat ccatcgctgc tcgtcgctcc
     2881 gcgtcgcggc gcgcattcgc cgtcctggcc gccgtcatgg tcgccttcgc caccctggtc
     2941 agcaccgccc cccaggccac ggccgccaac tacctctact ggagctactg gcagcagacc
     3001 gccgggaagt gggccttcag ctcggttggt gccgacaagg ccacgccgag cgacggtgcc
     3061 gtcgagggct ggcgttgggc gatcgacgac ggcaacggca gtcggccccc gcgcgtgctg
     3121 ccgaccttcg agcaactctg cgccagcacc ccggccgagg ccggcaagaa gcgggtcggc
     3181 ctcgtcgtcg acttcggccg cgacgtcgac ggggacggca aggtcactcc cccgtcgttg
     3241 gtcgcgacct gcgccctggt tcccacggca gccaccggcg ccgacctgct ggccaaggcc
     3301 ggcggcgtcc gcaccgacaa gggcttgatt tgcgcgatcg gcggctaccc cgccaccggc
     3361 tgcgccgccc agttggagac gctcaccgag gcccagaagg cgcccgacac ggccctgacg
     3421 cttcccacgg ccgcgccgac gacggcaccg acggccacca ccccggtagc gacgacgacc
     3481 accgccgcca cgcccccggc gaccgccacc gcagcagtcg tcgcgacgag caccacggcc
     3541 gcggccggtg ggacctcgcc gctgttgtgg gtcctgctgt tcgtggccgc cgccggcatc
     3601 gcgtatgccg tcgcccgtcg gcgccgcccc agcggagtct gaccggagcg acaccacatg
     3661 gcccgcccca cacccgccgc caccccccgg atgctgcatc cggtggcgtg gtgggtgtgg
     3721 ggcgtcggcc tggcgaccgc agccgcccac acgaccaacc cctggctgct cctgctcgtc
     3781 atcgcggtcg ccggttgggt cgtcctggaa cgccgtgaag tcggcgcgct ggactcgttc
     3841 cggcccttcc tcatcatcgg cctgtttgcc atcgggctgc gggtcgccct gacgatcctg
     3901 ctgggcaacg gcgtccccgg ccaggtcgtc cttgtcgaac tcccccaggt ccggctcccg
     3961 gactggttcg ccgggctgcg cctcggcggc cccgtgaccc tcgagtccgt tgtcgcggca
     4021 gggatcgacg ggcttcggct ggccacgacg ctggcctgtc tgggggcggc caatgggctg
     4081 gccagcccgc gacggttgct gcgctacgca cccgccaccc tctatgacat cggcaccgcc
     4141 atcgtcgttg ccctcacctt cgcgcccgcg ctggtcgacc tggccggacg ggtgcgttcg
     4201 gcccgacgcc ttcggggcca cagtggaacc ggtgtccgag agttggcccg gctgggcgtc
     4261 cccgtcctcg aaggggctct ggaccggtcc ctggacctcg cagcatcgat ggagtcacgc
     4321 ggctacggcc gcaccgccgt gcgtggtcgg cgcgaacgga tcgttggcag cgcactcgcc
     4381 ctcagtggcg catgcggttc ggtcgccggg ctcttcggcg tgatgtccgc gtcgtccggg
     4441 ggatggtttg gcctgccgat gctcgtcagc gggctcgccc tgctggccac ctcgctcgtc
     4501 ctgggctccc gcagagacgc gcggacggca taccgacgcg acccctggcg cgcacccgaa
     4561 tgggcgacct gcgcgctcgg tctgcttccg gccgtgctcc tcgtgcacgc cgaggccgac
     4621 gcctggccgg ggatcaccat cacccaggtc cccctcgact ggccgcccct gcccgcgctc
     4681 ccgaccctcg cgatcctcgc ggcggtgctg gtggcgtggg tgactcctgt tcctccccgg
     4741 ctggctcgag cgcgagccac cacccttccc gcccggctgg agaccgcatg atcgagcttc
     4801 gcggcgtcgg cgtcacgtat gccggtgcgt cccgtcccag cctgcgtgac gtggatctgc
     4861 gcatcgagga gggcgaactc gtcctcatcg tcgggccgac cggctcgggc aagtcgaccc
     4921 tgttgcggac cctcaacggg ctggtccccc acttctccgg cggaaccctc accggatcgg
     4981 tcgtcgtcgg cggacgtgac acccgggcgc accgaccgcg agatctggcc gacctggtcg
     5041 gcttcgtcgt gcaggacccg acctccgcct tcgtcaccga cacggtcgag gacgagatcg
     5101 cctacggaat ggagacgctc ggcgtcgagc cgaccgcgat gcgccggcgg ctcgaggaga
     5161 ccctcgacat catgggcctg gccgagttgc gcgatcgcag cctgcgggag ctgtccggag
     5221 ggcagcagca acgcgtcgcc atcgccgccg tgctcgccgc cggcccacgg gtgctggtgc
     5281 tcgacgagcc gacctcggct ctggaccccg tggctgccga agaggtcctg tcggcgctgc
     5341 accggctcgt ccacgacctg ggttacaccg tcgtcctcgc cgagcaccgg ctggaacggg
     5401 tcgtgcagca cgccgaccgg atcgtcctgg tccgcgacgg cgtcgtctcc gcgccgctcg
     5461 acccggccga ggcgatggcc ctctcccccg tgcatccgcc gatcgtcgag ttgggtcgac
     5521 ggatggggtg gtcaccgctg ccgctgacga tccgcgacgc ccggcgtcgg gccggctcgc
     5581 tgcgcgcccg gttggcggcc ctggacggcg ctcctgcggc ccacgcgccc gtcaaggacg
     5641 ccgcctcgcg atcgctggtg tcggtcaacc gactcgtcgt gcgccgcggc atcgtcgagg
     5701 ccctctccaa ggtcaccctc ggcgtgagcg ccgggtcggt cacggccgtc atgggccgca
     5761 acggtgccgg caagtcgacc ctcctggccg cgatggccgg tcagctcgcc gcgacctcgg
     5821 gcgaggtctc cgtggccggc tcgtcacccc ggcgcctcgc cccgcacgat cgggtccgcg
     5881 tcgtcggtct ggtcccccag gactccaacc tgctcttgta tgccgattcc gtggccgccg
     5941 agtgccgggc cgcagaccgg gacttcgccg cggcggacgg gacgacgcgc gcgttgctcg
     6001 cgcggatcgc cggcgaggtc gacggtgatc agcacccccg tgacctgtcc gaaggccagc
     6061 gactggctct cgcgctcgcc atcatcctcg ccggacgccc ccaggtcgtc ctgctcgacg
     6121 aaccgacgcg gggccttgac tacccggcca aacgccggct cgtcggcatc ctgcgcgacc
     6181 tcgccgccga gggccatgcc gtcatcgtgg cgacccacga tgtcgagttg gtcgccgagg
     6241 tcgcccagcg ggtcatcgtc ctggccgagg gtgaggtcgt cgtcgacggg ccgatccacg
     6301 aggtgctcac cggctccccc gcgttcgctc cccaggtcgc caagatcctg cacccgctgc
     6361 cgctgctgac cgtcgacgac gtcagtgccg ccctggtccc cgcgtcgtga ggtttcgccc
     6421 cgccgctgcc gcagcgctgg ccctcaccac cgtcgtcggg ctcgcggcat acctctggcc
     6481 gttcttcgcc gcaccgggtt tctcgacgag ctacggccag gacgcgccct ggctgttcgc
     6541 ggccctgctg gggttgatgg ccttcgtcgt cctcgccgag gtcaccgcga acggcctgga
     6601 cgccaagacc gtcgcggtgc tcggggtgct tgccgccgcg ggaggagccc tgcgtgtcct
     6661 gtcggccggc accgccgggc tggagccgat cttcgtcatc ttcgtcctcg gcggccgggt
     6721 cctcggtcgc tcgatgggtt tcctcatggg gtcgctggcg atgctcgccg gggccttcct
     6781 caccgccggg gtcggcccgt ggctgccgtt ccagatgctc ggcgccggat gggtcgcgct
     6841 cggtgcggcg atgctcccgc gggccacggg gcgagtggag ctcgcgatgc tcgccgggta
     6901 cggcgcgttc gccgggctcg cctacggcgt gctgctcaac ctgtggttct ggccgttcct
     6961 ggctcccgca tccgcgccca ccggtgccgg tttcgtgccc ggcgactcgg cggcggcgaa
     7021 cttcgcccac tacgccgtct tctacgtcgc cacctcgctc ggctgggacg tccctcgggc
     7081 ggtgctcacc gccgtgttga tcctcgttgc cggacgctcg atcctcgcga ccctgcgccg
     7141 agcggtccga cgggccgcct tcggcgaggt cgtccgcttc gactagtcct cgcgagtctg
     7201 cacctggacg aacggcgggt tcatcaccgt ggcctccacc ccgcgaccgc gcacgtcgat
     7261 gaggacctcg gcgtccaacg gcaccgaacg atccagcaac gcgagggcga tcccctgctt
     7321 gcgggtcggc gagaacgtcc ccgacgtcac ctccccgatc acctcaccat cgagcgtgac
     7381 cgggcagtgc gcccgaggta tgccgcgtcc ggtcaccagg agcccgcgcg agatccgcga
     7441 cgtgggcgcg gccttctcgg ccagcagcgc ctcccgaccc cagaaggccg gcttggccca
     7501 gcccaccgcc cagcccgacc gggccatgac aggggtgatg tccagcgaca ggtcctgacc
     7561 atgcagggga taacccatct cggtccgcag ggtgtcgcgt gcgccgaggc cgcacggcat
     7621 acctccccag gggagcatcg cctcgatgat cgcgtcccac aggtcggctg cgtcgtccca
     7681 ccgcgggacc aactcgtagc cgcgttcgcc ggtgtagccg gtgcgacaga cgatcacggg
     7741 acggccctgc cagtcggcct cgcggaactg catgtagtcg tgtccggtcg gcaggccgag
     7801 cgcgtcgagc acctcgtccg atcggggccc ctgaacggcg atcacggcat agtcccggtg
     7861 cctgtcgacc acctcgatgc cctcgggagc cgcggcctgc agccgacggc atacctctgc
     7921 ggtgttcgcg gcgttgggga tgaggaagac gtcgtcgtcg gcccgcaggt attggatgag
     7981 gtcgtcgacg accccgcccg tggcgtcgca gcacagcgtg tactgcgcct gacccggacc
     8041 gacccggcgc aggtcgttgg tgaagcacgc gttgacgaag tcggccgcac ccggcccgga
     8101 caccgtggcc ttgcccaggt ggctcacgtc gaagaccccg acccgctcgc ggaccgcggt
     8161 gtgttcggcg accactccgc cgccggggta ttcgatgggc atctcccacc caccgaagtc
     8221 ggccagtttg gcccccagag caacgtgacg gtcgtgcagc ggcgagtgcg cgagatcggc
     8281 catggccaac gagcctaacg ggccggaggc ggggtcgtca tggaccccgc cgctcgcgtt
     8341 catcgaggtt cgcttagccg ccggacttgc ggcggaacat ccgctgcgcc gcggccgggc
     8401 cgtgagcgtc gtggaccttg ccttcgccct cgcccgcact ctcgcggccg gcatgggcct
     8461 gcttgcgctc gagggcctcg cggaacttgg ccttcatctc gtccgagggg ccggcggcct
     8521 tgggggcctt accggacttg gcgttcttgc tcatgctcgt tcgtccttcc gtcagtcgtc
     8581 cgaccgggaa cccggtcggg tgtcgccgcg cttgtctcgg cgctggcgtg aactccattc
     8641 gcgcatccgg ctgggatagc cggtgaggag gacgtcgtaa atcgggatcc ccagagcggc
     8701 cgccatctca taggccgccg cacgcgaccc gacggcccgc cgagtccact ccccgtcggt
     8761 cgcaacgagc accacgcttt gggtgctgac gttggtggcc ggctcgagaa atgcctcgac
     8821 cccgcgtcgg gtttggacga attgggcgaa gtgcccgcgc acggtcgcca gatcgacgcg
     8881 catcgccggc ccgtcggatc gacccaggtc aggcagcgac gaccgacgcc atcggtcgaa
     8941 cagtcccata gtccgaacct tatgctgtcc gcgtgagcac tcggggcacc tccacagggc
     9001 gatgggcggg tgtcggcgtc gctggagtca tcatcctcag cctggccagc atcggggcgc
     9061 atgcccttct cattcctggt ttcatccggg cagccgacgc gtcctcacgc tcgaccggag
     9121 ctctgtccac gccgacgacg gtccagtccg ggcagggcgg cacggggtcc acgagctcga
     9181 ccagttcgcc cgccgcgacc gatctggccc cgaccgcggt gccggccatg ccggcatacg
     9241 accgggcctg cctgcgccgc gacaaccccg ccacgggagc gaagatcccc gcgaacgatc
     9301 cgttcgggga cgacatcgcc gacctgggcg acaaggccga gttgcagcgc ctgtatgccg
     9361 tgcagagcgg agcgctcgtc ccgctcgacg gcctcggggc gccacgaccg tgcgacgagc
     9421 agttgtggtc gctcgtcaag gccaccgccc ccaccctgct cggccacatc gacgagttgc
     9481 tcgtcttcga tgccgacccc gatccggcga cgggcgagtt catcatcgag ggcgagtcgg
     9541 cccccaagga gacggcgccc gacacgttcg acgacgacca ctggcgcgtg tcgttcgcac
     9601 ccaacgggct ggaccgcggc gagctcgcct ggctggtcgc ccacgagctc gcccatgtcg
     9661 ccagcctcaa caaggaccag atgctcagcg gcgtcggtgc cgacgtctgc gccacctggt
     9721 acaccggcac cggctgcctg ctggcggact cgttcctgcg gcgttacctc agcaacacat
     9781 gggacgacgc cctctgggat gactgggata aggccgacgg caagtccacc gaaaaggcgc
     9841 ggcgcgcggc ataccaggac ttctatgact cccacagcga ttccttcatc accgagtacg
     9901 ccgcctggca ccccctggag gacttcgcgg agtccttcgc catgtggtgc acctacgacg
     9961 aggacgagcc ggaccgggct aacctgccga ccgcagaacg gaccgacagc ggaaacaagg
    10021 ttgcctggtt cgatgccgca cggcgcgatc tcctgcccgc cttcgggccc ggctgcacga
    10081 tgctgcgaca gttcgccgtc agctgatcgc cacggcgcgt ccacccgaca cagttgtgac
    10141 acggcatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gaccatcgac
    10261 tacgtcgacg ggttcggcga ggtcacctcg cccacgacgg tccgtgtcgg cgaccgcacc
    10321 ctcacgggcc ggcgcctcgt gctggccacg gggtcgcgag cccgctcgtt gcctgggctg
    10381 gagatcggcg gccgggtgct caccagcgac caggccctcg gcctcacgca ggtgccgacc
    10441 tcggtcattg tcctcggggg cggcgtgatc ggagtcgagt tcgcctccgt tttccgctcc
    10501 ttcggcgcga gcgtgacgat catcgaggcc ctgccgcggc tggtgccggc tgaggaggaa
    10561 agcatctcca aggccctcga acgggccttc cgcaagcgcg gcatcgtcag ccgcacgaat
    10621 gcacgattcg cctccgtggc ccaggacgac gccagcgtca cggtcagcct cgaggacggc
    10681 tccaccctgt cggcagacct gctgctcgtc gccgtgggcc gcgaaccggt ctccgacggc
    10741 atgggttttc aggaggcggg agtcctcacc gaccggggtt tcgttccgac cgacgagcgc
    10801 ctgcgcacga acgtccccgg ggtgtatgcc gtgggcgacc tcgtccccgg cctgcagttg
    10861 gcgcatcgcg gcttcgccca cggagtgttc gtcgccgagg acattgccag tttgaacccc
    10921 gcccccgtgc tggaccgcaa catccccaag gtcacctact gcgaccccga gatcgccagc
    10981 gtcggcctca ccgaagcagc cgcccgcgca gagcacggcg aggtccgcgc ttacgagtac
    11041 aacctcggcg gcaacggcaa gtcccagatc ctcgggacca gcggattcgt gtccgtggtg
    11101 agagtggtcg acgggccggt ggtcggtatc catatgatcg gggctcggat gggtgagcaa
    11161 atcggcgagg cacaactcgt cgtcggatgg gacgccatgc ctgaagacgt ggccccactc
    11221 gtgcacgccc acccgaccca gaacgaagct ctcggcgagg cccatctggc gctggcgggt
    11281 aaacccttgc acgcccacgc ctgaccgcac cgagaacctt ttgcagcgca gacagattga
    11341 cgaccgagaa ccatcgacga ggagatgacg tagatgtcca atcgcgtgac catgccggcc
    11401 ctgggtgaat ccgtcaccga ggggacggtc acccgttggc tgaagaaggt cggtgacccg
    11461 atcgccatcg acgagccgct cgtcgaagtg tcgaccgaca aggtcgacac cgagatcccg
    11521 tcccccttcg cgggcatcgt cgaggcgatc ctcgtcgccg aggacgaggt cgccgccgtc
    11581 ggagccgacc tcgcagtcat cggcgacggt tctggggccg cggctgcgcc ggcggcgcct
    11641 gccgtcgaag ctgctccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt
    11761 gctccggctc ctgttgctcc ggctcctgtt gccgctgctc cggctgcccc ggctcctgtt
    11821 gccgcagcgc ccgctgccgc ggcccctgct gcttcggccg cgccggcatc ggcccgcccg
    11881 tcgctgcagc cgtcctcgaa gcgcggcacc accgagaaga tgtcgcggtt gcgcaaggtc
    11941 atcgcgtcgc gcatggtcga gtcgctgcag gtctcggccc agctcacgac cgtcgttgag
    12001 gtcgacctca cccgcgtcgc ccggctccgg gcacgttcga aggccgcctt ccaggcccgc
    12061 gagggcacca gcctgtccta cctgcccttc ctcgccctcg ccgcggtgga ggccctcaag
    12121 gtccacccga tggtcaacgc gagcatcaac ggtgacgagg tcatttacca cggtcaggag
    12181 cacctgtcga tcgccgtcga caccgaccgt ggcctgatcg tccccgtcgt caagaacgcc
    12241 ggtgacctca acatcgccgg tctcgcccgg ggtatcgccg acgtggcgaa tcggacccgc
    12301 accaacaaga tcacgcccga tgacctcacc ggtggcacct tcacgatcac caacaccggc
    12361 agccgtgggg ccctgttcga caccccgatc atcaaccagc cgcaggtggc catcctcggc
    12421 accggggcct tggtgaagcg tccggttgtg gtgagcgatg cagagggcgg cgagacgatt
    12481 gccgtccgct cgatgatgta tctcgccttg tcctacgacc accggatcgt cgatggcgcc
    12541 gacgctgcgc gcttcctcga gacgatgaag caccggttgg aagagggcaa cttcgagccc
    12601 gacctgggcc tgtaaggaac gtgtggtgag cggtcggcgg gttgccgtca ccggcgcttc
    12661 cggtttcatc ggtggcgcgt tgacggcata cctcgccggt cgtggcgacg aggtcgtgcg
    12721 gctggtccgg catcagccga cgcagccgtc ggagcgccgg tgggacccgg tgcgcggagg
    12781 cctcgacccg cgggcgatcg acgacgtcga cgcgatcgtc catctcgccg cggcgggtgt
    12841 cggtgatcac cgctggaccc ccgcctacaa ggaactcgtg ctccgctcgc gcgtcgacgg
    12901 aaccgccgcg gtcgctacgg cgatcgccca gacgggtcgg ccgatccggt tcgtgtgtgg
    12961 atcggcgatc ggctactacg gcgatcgcgg cgatgagatc ctcactgagc tcagcggccc
    13021 ggggaccggg ttcctcgccg aggtcgcccg ggcctgggag ggctccgccg aaccagcaat
    13081 cgctgccggc gcacccgtcg ctttcgcccg ccacgggctg gtcatgggtc cagggggtgg
    13141 cgcgttcgcc cgcctcttgc gcctggctcg ctgggggctc ttcggcccgc tggggtcggg
    13201 acgtcagtac tggtcatgga tcaccctggc tgacagcgtc cgggcgctca gtcatctcat
    13261 cgaccgaccc gacctcaccg gcccggtcaa catcgttggc cccgaccctc gcccccaacg
    13321 ggttgtggcg agcgcgatcg gtcacgccct gcatcggccg gcgatgctcc cggccccgtc
    13381 ggtggccctg cgggtggcgc tcggcgagtt cgcggacgac atcctgggct cgcagcgggt
    13441 gatgccgttc gcgctcaccg gaagcggatt caccttcgaa cactcgatgt tgcgcccggc
    13501 cgccgcatgg ctggtccgcg aatcctcgtg actcgctgac ctgccaccct ctttgatctg
    13561 ccggccactc ccacggtcat cccgtgacag ttgagatccg ccattcctcg ccaacccgca
    13621 ccagcgagta gagcagcgat cggccgctgt cggccccgat ggattgttcg cgatcacccg
    13681 gaccctgcac gcgatacccg ctgcgttcga cgacggcccg gatgcgcgct gacccggcat
    13741 ccacgctgac ccattcggcg acggtgaccg tgaaggtcag cccgacatag cgctgctcgg
    13801 cctgaaccaa ctcggcgatg atggcctcat cggcggccag caacgtggaa ccctggccgt
    13861 cagcggcggc gagcgcccgc aggtctcggg cggtcagcgc ctgggcccgg cggccgacca
    13921 gggtggccag caccgccctg gcctcacccg ctggtgccgc cgcggagccc agcacccgag
    13981 tgacctctcc tgccgaaccc tccgccgcgg cggccggact caagcggagg aacacgccga
    14041 cgacgactgc ggccagcacg atggccaggg ctgctccgag acccagccgg gccttgcgcc
    14101 ccaccctcgg acccggcacc ctgccgccgg cccggcccct gcgcgctgcc tggccccggc
    14161 gcactgcctg gctcctgccc ggacctgccc cgacgcccgg tgtcacccga cccgccgcgc
    14221 ctcgcaggcg ttgggtgagg acccggtcag gatcggcacc cgccgccgaa cccaccaggc
    14281 gaacggccac cgggctggcg gcttgatagg ccagcacggc ggcctcctgg gcctccgggc
    14341 gaccggacgg atcttgcgag agcatcgccg ccaccaccgg cccgaacccg gcacccagca
    14401 ctgccacagc ttcatccacc gtcaccgggc ccggcaccga cgtgtccgcg ccacccaacg
    14461 gcgttgccca ggaccggacc gaccaggatc ggccggtcaa cgcaaaccac gccagcgccc
    14521 ccaggccgaa gacgtccgcg gcctcaccgg gcacatctcc cgcgaccacc tcgggcgcga
    14581 cgaaacccga ggtcccagcc acgagcggcg ggtgaccctc ccccactagc cgggcggccc
    14641 caaagtcgct cagcagcggt ttcccgtcag ccgtgaacag gacgttcccc ggcgagagat
    14701 cgccgtgaac gacgccggct gcgtgcaggg cggccagggc gccggcgagc ggtgtgcaga
    14761 tcgtcgccac ctcaccgcca tccaggcgcc cacgggccgc caccagatcg gcaaggctgc
    14821 cccccggtgc cagatcaagg acgaccgcga ccgacccgtc cgggagggca tgggcctcgt
    14881 gcaaccgcac gaggtgatcg tgctcgacgg tggccaggat ggcggtctcc cgggccgcca
    14941 actccatgag ttggccgacg tgcaaccgat cgggcgacgt gatcttgacg gccacggccc
    15001 gcccgtcatc gacccggacg gccgaccaca cggtgctcgt ggccccgcgc ccgatgacct
    15061 ggccgaggac cagcccggga accgagggtg ggatcgtctt ggcagcgctc acccactcat
    15121 ggtgccgctg acgagcgatc ctccgcagaa gttatccaca gccctgctgg cacgcttgga
    15181 cccaggtgac accccggcct cgcccatcca tctggcgtgc gatcagtgcg ggtcttcgcc
    15241 gggggtgatg acgtcgacga cccggccgtc ggcggcgctg atccgggccg cgtcgatgac
    15301 ggccagcacg tggaccgcgt cgcgggggtc gaccggcata ctctcctgcg ggtccggcga
    15361 cgcgagggct gcggccacct cgcggtagaa gtcggccggg tcgctcgagc gggcgaggac
    15421 cgcctcccgc tcgtcgccac gcacgagcca gccgtgttgg cccgggccgc cgtcggcgtc
    15481 gtggaacgcg gtgggctcgc cgccggccga gccgagcagg aacgtcccgg cgctccccgt
    15541 gatccgagcc cgcgggcccg gggctcccgc cacactgctg gcgctcaggt gtgtggagta
    15601 gccgtcggta tgccgaagcg cgacgaaggt gtcgtcttcc gcggtcaccg tccaggctgc
    15661 gagctcggca tataccgtcg ccaccggtcc gtgcatgagg acaacttggt cgagcaggtg
    15721 cgtgtgcaga tcgaggagca agccgccgcc ctcggccgcc gtggcgcgct cgcgccagcg
    15781 gtccttgggg accggccgcc agcgatccca gcgcatctcg gtgcgacgca gttcgccgag
    15841 gaccccgcga tgcagcaggt cgcggatcgt cgcgaactcc gggtcgtagc gacggttctg
    15901 gaacaccgtc agaggcacgc cgcgacccgc agccaggtcg accaagccct gggcctggtg
    15961 ggcgtcggtg gcaatcggct tgtcgacgac gacggcgatg ccggcctcaa ggagcgcctc
    16021 ggcatgctcg acgtggcgac cgctcgggga cgccacgacg acgacatcga ggtcgggcac
    16081 ggccaccagc gcggccaggt caggcacgac gacgacgccg gggttctcgg cccgcgcctg
    16141 gtctgcgcgc tccgggttgc ccgagacgac ggccacgagg tccagccccg cctcccgcag
    16201 caacggcgca tggaagcccg cacccgacga gccgtatccc accagtccaa cgcgagtcat
    16261 cacgcgcctc ccttcccgcc gcggcggcga ggtgcccacc ctaccgaggc gggcggcata
    16321 caccctcggg tatgccgtcg cgccgggtta aggtgggcct catgcgcgtc gtccacctcg
    16381 gcttcgatcc cgacttcgtg gactacgaat gggcgtgggc gcatcagcgc gaggtgcacg
    16441 cgaaggtcgt gtccggggag gaacccgaca cggtgctgct cctcgaacac caacccgtct
    16501 acacggcagg gaagcgcacc gaggcgcacg agcgaccgaa cgacggcaca ccggtcgtcg
    16561 atgtcgaccg aggcggcaag atcacctggc acgggccggg tcaggtcacc ggatatccga
    16621 tcgtccgcct gccggccccg atcgatgtcg tcgcctatgt gcgactcctc gaggagatga
    16681 tgatccgggt ctgtgccgac ttcggggtgt cggccgggcg cgtggacggg cagtcgggga
    16741 cgtggatcct cgccgacccc gcagatcccc ggcgtcggcc gaaccgcaag atcggcgcca
    16801 tcggcatccg ggtcagtcgt caggtcgcga tgcatgggtt cgcgttcaac gccaacccgt
    16861 cgctggcgtg ggccgacgtc atcatcccct gcggcatccc cgacgccggg gtcacgtcgc
    16921 tgtcagccga agtgggccgt gacgtgccgg tccgcgaggt cctcccccac atcgagaagc
    16981 accttgccga cgtgctgggc acgttggcgc caaaccgtcg ggcgatctga gctcacgccg
    17041 gacgtaaact gggagacccg cggacggcat actcctgacc cgttgcggca ggaaggacgg
    17101 acccggtgac gatcgcaccc gaggggcggc ggatgttgcg cgtcgaggcg cgcaacgcgc
    17161 agaccccgat cgaacgcaag cccgaatgga tccgcacgac ggccaagatg ggcccggcct
    17221 tcaccgaact gcattcgatg gtcaagggcg agggcttgca caccgtgtgc caggaggccg
    17281 cctgccccaa catcttcgag tgttgggaag accgtgaggc gaccttcctc atcggcggcg
    17341 acacctgcac gcggcgatgc gacttctgtg acatcgcgac cggtcgcccg cagccactcg
    17401 accccgacga gccgcgccgg gtggccgagt cggtgcgccg gatggggctg cgctacagca
    17461 cggtgaccgg tgtcgcgcgc gacgaccagc cggatggggc ggccggcctg tatgccgcga
    17521 cgatccgcgc gatccacgaa ctcaacccga acaccggtgt ggagatcctg cccccggact
    17581 tcggcgccaa gccggagttg gtcggccagg tcttcgacgc ccggccggag gtgttcgcgc
    17641 acaatctgga gacggtgccg cggatcttca agcggatccg tccggcgttc acctacgaca
    17701 cgtcgctgcg ggtgttgtcg atggcccgcg aggctggtct ggtcaccaag agcaacctca
    17761 tcctcgggat gggcgaggag gcgcaggaga tcgaggcggc catcgtcgac ctgcatgacg
    17821 ccgggtgcga catcctcacg atcacgcagt atctgcgtcc gtcggccaag catcacccca
    17881 tcgaccggtg ggtcaagccc gaagagttcc tgcactggtc gtcggtcgcc accgagcacg
    17941 gcttcaaggg cgtcatggcg gggccgttgg tgcgctcgtc gtatcgcgcg gggcggctgt
    18001 gggcgcaggc gatggcgcgg tggggtcggc cggttcccga gcatttggcg cacctggcgg
    18061 aggctgccgg agccccggcg cgtcaggagg ccgcagccgt ggtcgcggca ttcgcagagc
    18121 gctccggcgc ccccgtatcc tgactcccat ggccaaggac tcctccagca ccgccgcccc
    18181 gaagaagccc gggcggatcg ctcaactgcg caccgtcctg cagcagtcca aggcgctcga
    18241 tccgaagatc gtgtggtgga tggcgggggc gttcgtcctc acgctcgcgg tcatcgtcgg
    18301 catcgggtgg ctggtcaact ggccggtgta tgccgcgatc ctcggcctcc cgttggccgc
    18361 cctggcagcc accatcgtca tgagccgccg agccgagggc gcggcatacg cgaagcttga
    18421 ggggcagcct ggggctgcag gtgcggcgct ctcatcgctg cgtggcgggt ggttcacctc
    18481 gtccgagccg gtggccgtcg agggtgcccg gtcggggagc atggccgacg ccgccctcgt
    18541 ctatcgcgcg gtcgggcgcc cgggtgtcgt gctggtggcc gaggggcctg aggcgcgggc
    18601 gcagcggctg ctgctggctg agaagaagcg caccgagcgg gtggcgcctg gggttccggt
    18661 gacggtctat cgggtcggtt cgggtaagga ggagggggtc gtcgagatcc gcaaactcac
    18721 ctcgaaggtg cagcggatgc gcgcgacgct ctcgaaggac gacgtcctca aggtcaacca
    18781 gcggctgcgg gctctgggcg gcgtgaagct gccggtgccg cagggcatcg acccggcgcg
    18841 ggcacgcccg gaccgcaagg gtatgcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnngcg gtgtcgtcct gccaggggtg gcgctcgggt cggaagagtt ggaccgatcc
    19021 cacggggacg aagccgacgt gcaggaagag ccgcagggac cgggcattgc cgggggcgac
    19081 ggcggcgacg agcgggtcag agtgcccgag ttcgtcatcg acccaccgca gggcggcggc
    19141 gagcagttcg cgccccgatg tcggcccttc ggtctgcagc ccgatctcgg gtagtccacc
    19201 cagcccggac gcgacggtga cgaccgagcg gacccggcgg gacggcatac cgaggactct
    19261 gatgccgctg cggacgtgct gggcgacggc gacccgcggg tgcccggcca ggtcgtccct
    19321 ggacacgagg tccgggccgg tcgcggtggg gcggggggcg tccggagggc ggacgagcag
    19381 gacgtcgagg gcgtccgccc aacccgatcc gcggatgagc tcggcgaccc gcgggtggtg
    19441 tgcgccgccg tagccgtcga cgccgtaggc gtccaactcg acgtcggcaa ggtcctggcc
    19501 caccgccagg taggcgtgcc cagtcatggc aacgaccgcc tcgactccgc gcaaccacag
    19561 cgaggctcgc tcccagcctc cgtcggccgc cgggaattct cccttggccg ccgcccgcag
    19621 gacgtctgcg aggtcggccg gcgagccgat caccggcgca cgagcacggt cgccgccgcc
    19681 ttgtcgtgca gaccgcgact atcgcggtcc cagatgaggg cgggcacggc aaggcacagc
    19741 agcagcgtcc gcagggtcgc ttggacgacc gagatccggc gtcggtcgac ggtcatgatg
    19801 acgatcccga gcagccgctg accgacggtg gcgccgaggg tgggcagcag caggagattt
    19861 tcgacggcga agacggccaa cggcgcgaaa ctgccggcgc cgccctgccc gagctcggcg
    19921 cggaacacga agacggcgat gagctggcac aacgcccagt cgatgcagat tgcgacgagt
    19981 cggcgaccga accgggcgat cgatccgggg ccggatggcg gcatacccag gtcttgtccg
    20041 gggtactcgc ctggagcctg agtgcgggcc cggggccctt ccaaccagga ggcgacgtca
    20101 cgacgatcga ccaccccgat agcctagtcc gcagcacaac gtcgtgcgcg cacgcacccg
    20161 ggctccagtt aacaggggcg aaacatcggc aacacgaccg ggaaatccgt ccgccctagc
    20221 gtcggggcga agcaccacac cgggcgtcgc attgcgttgt gtcctgcggc gatccgggtc
    20281 cactctggag gaggttggtt tgttcacttc agccgacgag gttctggcct atatcaaggc
    20341 cgagaacgtc aagttcgtcg acatccggtt ctgtgacctg cccggggtca tgcagcactt
    20401 caacgttccg gcgcacacgc tggacgagga cttcttcacc gtcggccagg ctttcgacgg
    20461 cagctcgatc cgcgggttcc aagcgatcca cgagtcggac atgaagctcc tgcccgacgt
    20521 ggcgacggca tacctcgacc cgttccgcgt cgagaagacc ctggtcatca acttctcgat
    20581 cgtcgacccg ttcaccgacg agccctacag ccgtgacccg cgcaacatcg cggcgaaggc
    20641 ggaggcctac ctcaagtcaa cggggatcgc cgacacggcc ttcttcggcg ccgaggcgga
    20701 gttcttcgtg ttcgacgacg tgcggttcaa gacgtcggcc aacgagagct actactacgt
    20761 cgactccaag gccgccgtgt ggaacaccgg caaggtcgag gagggtggca acctgggcta
    20821 caagccgcgc atgaaggggg gctacttccc ggtgtcgccg gtcgaccact tcgccgacct
    20881 gcgtgacgag atgtgcctgc ggctggccga ggtcggcatg gaggtcgagc gttcgcacca
    20941 cgaggtcgcc tcgggtgggc agcaggagat caactaccgc ttcaacacgc tgctgcactc
    21001 cggcgacgac atgatgaagt tcaagtacgt catcaagaac ac
//
LOCUS       DAORPW010000030        20607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_441_length_20607_cov_697.817098, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000030 DAORPW010000000
VERSION     DAORPW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 20607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20607
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_441_length_20607_cov_697.817098"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PLB86_04275"
     CDS             <1..987
                     /locus_tag="PLB86_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC64385.1"
                     /translation="VGSAAGVGAAGALGVWALARRSIRSALVAAPLVVVGSVAAGVLV
                     SSRAMFISDHDSSVVLLVVAAVAPVALVYGLVLASRVHRLDRAAAERAAEAVAAHERD
                     AAVEAQRRDLVAWASHDLRTPLAGIRAMAEAIEDGVAPAEGAYPARIKAQADRMAAMV
                     DDLLALSRIHSGTMTLAREQVSVADLVSDALATGAALAERSGVLVDGAADGPVLARVD
                     ARELGRVLDNLIANAVHATPRGGRVVVHAAASPTQVTIRVTDECGGIPADVRARMFEP
                     WWRGDDARTPRAGDGAGLGLAVVQGIVSAHGGTVGVADVPGGCAIEVVLPRD"
     gene            complement(1002..>1645)
                     /locus_tag="PLB86_04280"
     CDS             complement(1002..>1645)
                     /locus_tag="PLB86_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HRC64386.1"
                     /translation="RTQVRWVTRNRRALVITASLGASITLFTLILKATTALLVIDPRT
                     WSILSGVILVLLGIAGAFPDLWERLSATLSLQGRSDARLRAARARGGLTGDILTGAAL
                     GPVFSSCSPLYAYVVVTVLPARFGYGLTLLAAYVLGLCVTLLAIALLGRRLIARLGWA
                     ADPHGRFRRILGLVFILVGIGIALGWDRSLQTWILEHSPIAPWELDSGFIPEG"
     assembly_gap    1646..1745
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1746..2043)
                     /locus_tag="PLB86_04285"
     CDS             complement(<1746..2043)
                     /locus_tag="PLB86_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64387.1"
                     /translation="MTSRTLSRPQFTAAALALGTALTLSACGGSATPLAAPAASTAMS
                     DKTAMPDKTAMPDKTAMPDKTGAPAAMAKGAYVTYADYQSSMAMYQGSKVVLFFH"
     gene            complement(2113..3078)
                     /locus_tag="PLB86_04290"
     CDS             complement(2113..3078)
                     /locus_tag="PLB86_04290"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRC64388.1"
                     /translation="MTTVDPRAELASHPGSTGRGFGFVVGQYVALTKPRIIELLLITT
                     VPVMVLAERGIPSGWLVLATLVGGTLSAGAANTLNCVIDRDIDALMNRTASRPLVTGT
                     ISTRAAVIFGFTLCAVATLVLGVFVNWLSAWLALAAFAFYVGIYSVVLKRRTPQNIVW
                     GGAAGCMPVLIGWSAVTNRVDWPAWILFLVIFFWTPPHYWPLSMKFKDDYAAASVPML
                     PVVARMVVVSRQIVIYSWVMVLVSLTLIPVAPMGWVYAVGAVVSGGLFIAEAHRLHHQ
                     ALAGAPQAQLRPMRLFHYSITYLTVLFVAVAVDPLLYLAIPGLAR"
     gene            3251..5377
                     /gene="tkt"
                     /locus_tag="PLB86_04295"
     CDS             3251..5377
                     /gene="tkt"
                     /locus_tag="PLB86_04295"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRC64389.1"
                     /translation="MATAVGWTDLDIRAVDTARLLAADAVQKVGNGHPGTAISLAPAA
                     YLLYQHVMTHDPSDPHWLGRDRFVLSCGHSSLTQYVQLFLSGYGLELSDLQSLRTWGS
                     LTPGHPEVNHTPGVEITTGPLGSGLASAVGMAMAQRRQRGLLDPEAAAGESIFDHHIY
                     VLASDGDIMEGVTHEACALAGHQELGNLTVIYDQNQISIEDDTDISFSEDVAARYVAY
                     GWDVRTVDWRGATADGATYVEDIDALNAAIEAGRAITDKPSLIVLRTIIAWPSPTKQN
                     TGKSHGSALGADEIAGLKTALGFDPEVAFPVAPRVLTQARKVVARGKAAHKAWDKRYA
                     AWRKAQPERAALLDRIVERGLPTDLAAALPSWPADAKGIATRAASGKVLSALAPVLPE
                     LWGGSADLAESNNTTMAGEPSFIPARRQTHEWKGGPYGRTLHFGIREFGMGAILNGIA
                     LEGLTRPYGGTFLVFSDYMRGAVRLAAIQQLPVTFVWTHDSIGLGEDGPTHQPIEHLA
                     ALRAMPGLDVVRPADANETAYAWLEILRNARPAALALSRQNLPVLDRKVHAPARNVAK
                     GGYVLIDGGADGRQAAEVILVATGSEVALALAAREQLERARIRTRVVSMPCREWFDQQ
                     PKSYRDRVLPPTVRARVSVEAGVAQGWRDIVGDAGRIVSIEHFGASADAATLFREFGF
                     TPEAIVAAAKASLKTARAGARKEGLT"
     gene            5374..6489
                     /gene="tal"
                     /locus_tag="PLB86_04300"
     CDS             5374..6489
                     /gene="tal"
                     /locus_tag="PLB86_04300"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRC64390.1"
                     /translation="MTAASTDNLADLAAAGVSIWLDDLSRSRLTSGSLAALIRDQHVS
                     GVTTNPSIFQAAITSGTDYDEQLASLAAAGADADAALFAITTDDVRAGCDVFASVARD
                     TNGIDGRVSIEVDPRLAHQTGPTIEQAKALAAAVGRDNVYIKIPATLEGLPAIAAVLA
                     EGISVNVTLIFGLQRYRAVMNAFVEGLERAHEAGHDLRTIHSVASFFVSRVDTEIDGR
                     LEAIGTDAARALKGQAAVANARLAYQAYEEVFTTPRWRSLADEGARRQRPLWASTGVK
                     DPAYPDTLYVVDLVAPDCVNTMPEKTLRAVADHGIVRGDTITGEYAAAQGVLDAVEAQ
                     GISYIEVVELLEREGVEKFEKAWVSLLADVTGALARA"
     gene            6562..8103
                     /gene="zwf"
                     /locus_tag="PLB86_04305"
     CDS             6562..8103
                     /gene="zwf"
                     /locus_tag="PLB86_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRC64391.1"
                     /translation="MSPARVSAGVNPLRDRRDRRLPRIAGPCGLVLFGVTGDLARKKL
                     MPAIYDLANRGLLPPGFSLVGFARRDWADQDFGQIVHDSVRDHARTPFREDVWKSLAE
                     GIRFVPGTFDDDAAFDTLAATVHELEQARGTGGNLAFYLSIPPGFFAQVCQQLDRSGL
                     AHSDGRSWRRVVIEKPFGHDLASARELNAIVESVFPADSVFRIDHYLGKETVQNLLAL
                     RFANQLFEPVWNANYVDHVQITMAEDVGIGGRAGYYDGIGAARDVIQNHLLQLLALTA
                     MEEPVSFDAASLRAEKEKVLSAVVVPAKIGEGTARGQYAAGWQGGELVPGYLEEEGVR
                     TGSTTETYAAVRLEVATRRWAGVPFYLRTGKRLGKRVTEIAVVFKKAPHLPFDTTATE
                     ELGQNAVVIRVQPDEGITMRFGAKVPGAQMEVRDVTMDFGYGSAFTESSPEAYERLIL
                     DVLLGEPPLFPRHEEVELSWQILDPIEAWWRRHGKPDPYPSGGWGPASSDRMLARDGR
                     VWRKP"
     gene            8100..9254
                     /locus_tag="PLB86_04310"
     CDS             8100..9254
                     /locus_tag="PLB86_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HRC64392.1"
                     /translation="MIVDLPSTSTAAIGKQLVQLRNEVGALALGRVLTLIVVVDDDAA
                     DEAVEIATEASRQHPCRIIVVISGSRRGANRIDGQIRVGGDAGASEVVIMRLFGKLAE
                     HGQSVVTPLLLPDSPVVAWWPREAPSDLRHDPIAAMATRRVTDSAECSHPRAMLTRLA
                     ETYQPGDTDLSWTRITLWRGLLAAALDGPPYESVTAATVAGAPDSPSTDLLAAWLAWA
                     LRVPVTREDTQAGSGICAVGLERASGAIDLARPGDLVATMTQPGQPDRRISLRRRGDA
                     ECLADELRRLDADETYADVLRKGLPALGTTKGASRSAAVSESKREAERERRNHAAQLR
                     TAAAEAGSLPSTVDTTTPAKPSGSTPTKPRVTRSTSATARTGRTTKEQPS"
     gene            9251..10000
                     /gene="pgl"
                     /locus_tag="PLB86_04315"
     CDS             9251..10000
                     /gene="pgl"
                     /locus_tag="PLB86_04315"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRC64393.1"
                     /translation="MSEHPTNAPLVVVHPDKQGVSDAAGARLLLALLDTLCVRDDAHV
                     VLTGGSLGSDMLASAGRSPLARLVDWSRVHLWWGDERYLPDGDPDRNETQNRAALLDH
                     LPLDPAKIHGMAGPDRSESAEASAAAYATELRTHGGGLFDVVLLGMGPDGHVASLFPQ
                     HPAQLVSDAIAVAVHDSPKPPPDRVSLTFECLARSRQVWLLVAGADKAPAVCRALTPG
                     AQRWDVPAAGVRGLDATLWLVDVEAASELPS"
     gene            9997..10452
                     /locus_tag="PLB86_04320"
     CDS             9997..10452
                     /locus_tag="PLB86_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRC64394.1"
                     /translation="MITDPAVEFVGELLATGGTTTGFDVPESVVTALGSGKRPKVLVT
                     VGGHSWQTSIAPMGGRFMLGVSAANRAAAGLEAGQTVPVTVVLDTAPRVIEVPSDLAE
                     ALAAAPDAAAGWARLSYSHQRAHVEALLAAKKPETRASRLTKTLAMLTA"
     gene            complement(10571..10921)
                     /locus_tag="PLB86_04325"
     CDS             complement(10571..10921)
                     /locus_tag="PLB86_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HRC64395.1"
                     /translation="MASGNAIRGSRVGAGPMGEAERGEAAPRVHISYWCTNGHETRPS
                     FAQEPGMVAPEHWDCPRCGLPAGQDRENPPAPPKVEPYKTHLAYVKERRSDADGQAIL
                     NEALGALRQRGLIQ"
     gene            complement(10937..11185)
                     /gene="secG"
                     /locus_tag="PLB86_04330"
     CDS             complement(10937..11185)
                     /gene="secG"
                     /locus_tag="PLB86_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRC64396.1"
                     /translation="MTVVKIALQALLVLSGLFLTLLILMHKGKGGGLSDMFGGGMSSS
                     LGGSTVAERNLDRFTIAVAILWFTAVVGLGLVERFVAA"
     gene            complement(11390..12178)
                     /gene="tpiA"
                     /locus_tag="PLB86_04335"
     CDS             complement(11390..12178)
                     /gene="tpiA"
                     /locus_tag="PLB86_04335"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRC64397.1"
                     /translation="MSRIPLLAGNWKMNQDHLQATHLVQKLDWTLRDGKHDFGAVEVA
                     VLPPFTDLRSVQTLVEGDKLSVRYGAQDLSVHDSGAYTGEISGAFLAKLGCTYVTIGH
                     SERREYHAETDAVINDKVKAAYRHGVTPILCCGEGLDVRKAGGQVAHVVAQLTRDLEG
                     IPAEQARTIVIAYEPIWAIGTGEVATPEDAQEVCAAIRTLLAELYSGDLADGVRVLYG
                     GSVKSSNVAAIMAKEDVDGALVGGASLDAAEFAAICRFKDHVTG"
     gene            complement(12201..13403)
                     /locus_tag="PLB86_04340"
     CDS             complement(12201..13403)
                     /locus_tag="PLB86_04340"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRC64398.1"
                     /translation="MKTIAGLGDLRGKRVLVRSDLNVPLDGDKNITDDGRIRASVPTI
                     KALAEAGARVVVCAHLGRPKGAPEDKYSLAPVVGRMSELLGQKVAFATDTVGESAQAV
                     VAGLADGDVALLENIRFNAGETSKDEGERAAFAAQLAALADAYVSDGFGVVHRKQASV
                     YDIVRLLPSAMGGLVAAEVEVLKRLTEDPARPYAVVLGGAKVADKLAVIENLMKTADR
                     LLIGGGMAFTFLAAEGYEVGSSLLDKDNIETCKGYLARAKESGVEIILPVDVVAATAF
                     SADAEHDVVPADGIPADRMGLDIGPRSAELYASKVRDAKTVFWNGPMGAFEMAPYAAG
                     TAAVAKALVEATADGALTVVGGGDSAAAVRQLGFTDDQFGHISTGGGASLEYLEGKEL
                     PGIAILNG"
     gene            complement(13449..14456)
                     /gene="gap"
                     /locus_tag="PLB86_04345"
     CDS             complement(13449..14456)
                     /gene="gap"
                     /locus_tag="PLB86_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRC64399.1"
                     /translation="MTVKVGINGFGRIGRNFYRAVVESGADIEVVAVNDLTDNKSLAH
                     LLKYDSILGRFPGEVTYDEASITANGKTFAAWAERDPAKLDWASVGADIVIESTGFFT
                     DATKAKAHVDGGAKKVIISAPASNEDITIVMGVNDDKYDPALHTVISNASCTTNCLAP
                     MAKALNDGLGIVKGLMTTIHAYTQDQNLQDAPHKDLRRARAAALNMVPTTTGAAKAVA
                     LVLPELKGKLDGYAMRVPTPTGSATDLTFEAARETTVAEVNAIVKAAAESGPLKGYLV
                     YTEDPIVSKDIETDPASCIFDAPLTKVIGNQVKVLGWYDNEWGYSNRLVDLCALVGSK
                     L"
     gene            complement(14782..15759)
                     /gene="whiA"
                     /locus_tag="PLB86_04350"
     CDS             complement(14782..15759)
                     /gene="whiA"
                     /locus_tag="PLB86_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HRC64400.1"
                     /translation="MAMTARVKDELSRLQIAKPCCRKAEVSATIRFAGGLHIVGGQIV
                     IEAELDTANAVRRLRRDIAELYGHRAEVVVMSGGGLRRGSRYVLRVVDDGAILARQTG
                     LVDGRGRPVRGLPPKVVSSSACDAEAAWRGAFLAHGSLTEPGRSSALEVTCPGPEAAL
                     ALVGAARRLGIAAKAREVRGVDRVVIRDGDAIAAMLTKMGAHDAVLAWEERRMRREVR
                     ATANRLANFDDANLRRSARAAVAAGARVERALEILGDEAPDHLKEAGALRLQHKQASL
                     EELGELASPPMTKDAVAGRIRRLLAMADKKAADLGIPSTEASLTPDMLE"
     gene            complement(15927..16865)
                     /gene="yvcK"
                     /locus_tag="PLB86_04355"
     CDS             complement(15927..16865)
                     /gene="yvcK"
                     /locus_tag="PLB86_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HRC64401.1"
                     /translation="MNGPRVVALGGGHGLAASLAALRLVTDRVTAVVTVADDGGSSGR
                     LRDEFNVLPPGDLRMALAALCDDRPWGQIWRDVLQHRFGGDGPLGGHAMGNLLIVALW
                     DQLGDTVAGLDLVARLLDSRGRVLPMAGEPLEIEADVVGADPDRPDDMSVVCGQHRVA
                     STRGRVSSIRLRPAQPQPCPEALAAIDDADWVLLGPGSWFTSVLPHVLVPDVRDALVR
                     TSGKRVLNLNLAVHQGETKGFRAVDHLTSLGRHAPELRLDVVLADPSVLDDADEVAAV
                     ASGLGARLVTSPIAATGRPGQHDALRLAAAYRDLMG"
     gene            complement(16862..17800)
                     /gene="rapZ"
                     /locus_tag="PLB86_04360"
     CDS             complement(16862..17800)
                     /gene="rapZ"
                     /locus_tag="PLB86_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRC64402.1"
                     /translation="MDEHAPEHVPVEGLRAVPADGGPAPLVVVTGMSGAGRSTVAKAL
                     EDHGWHVVENLPPQLLPDLESLHERARPPADGGPSPRLAVVIDARTRWLSGQDAHLLE
                     QLTSGPHRPVLLFLDATDEALVRRFESVRRPHPLQGNGRLLDAIAAERTLMADLRSEA
                     DVIVDTSGLNVHQLHAKVSLLLDATVGSRVRLAVMSFGFKYGVPLDADFVLDMRFLPN
                     PFWVPELRAKSGIDTDVADYVLGQAGATEFLDRAIALFDPVLAGYQREGRRYVTLAIG
                     CTGGKHRSVAVTEALAARFDPERVVAFVVHRDLGRE"
     gene            complement(17816..19870)
                     /gene="uvrC"
                     /locus_tag="PLB86_04365"
     CDS             complement(17816..19870)
                     /gene="uvrC"
                     /locus_tag="PLB86_04365"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRC64403.1"
                     /translation="MADPSTYRPAPGQIPLDPGVYRFRDASGRVIYVGKAKSLRPRLS
                     SYFQDIAALHPRTATMVRTGASVEWTVVGNEVEALQLEYAWIKEFDPRFNVRYRDDKS
                     YPFLAVTLGEQFPRAQVLRGAKRKGTRYFGPYSHAWAIRETLDLLLRVFPIRTCSTGV
                     FKRAAQSGRPCLLGYLDKCAAPCVGRVTEDEHRAIAEEFCDFMAGNTTRFVRRTERAM
                     HDAAAELEFERAARLRDDLVALTKALEKNAVVLGDATDADVFAIVDDELEAAVQVFHV
                     RGGRVRGQRGWVGEKEHEDLPDLVEHLLQQVYGDQPPDAVPREVLVPVLPSDPGPVAS
                     WLATRRGAAVDLRVPRRGDKRALMETVERNAAQSLARHKLSRAGDLTARSVALQELQE
                     ALDLRDAPLRIECFDVSHVQGTNIVASMVVFEDGLARTGEYRRFLVGGPDPQPDDGPA
                     GQLHAEATVDDTAAMREVLTRRFSRLVAAESAAAQEAQEAAEAAALSGSSGEPGGMPL
                     VPGLDPQTGRPRKFAYRPNLVVVDGGLPQVTAAAAALRELGITDLALVGLAKRLEEVW
                     VPGQDFPIILPRTSEGLYLLQRVRDEAHRFAITFHRQRRAKAMTASVLDGIPGLGPSR
                     RAALLARFGSVKRLRAATPEEIQEVPGIGPSLATAIVVALARDTATKPASVNVTTGEI
                     LD"
     gene            complement(19984..20394)
                     /locus_tag="PLB86_04370"
     CDS             complement(19984..20394)
                     /locus_tag="PLB86_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HRC64404.1"
                     /translation="MATQPSRRSILQVAGTVGAAAATAGTLAGCGGSSAAPAQTTKAA
                     GSALKAADIPVGGGKVVGQVVVTQPTAGTFKAFSAVCTHEGCLVSKIESAKITCTCHG
                     SVFDAATGAVVSGPAKGPLAAKTVTVSGDSLTLS"
BASE COUNT         3122 a         7127 c         7208 g         3050 t
ORIGIN      
        1 gtgggttccg ccgcaggcgt cggcgccgcc ggagcgctcg gcgtgtgggc gctggcgcgg
       61 cgctcgatcc ggtcagccct cgtcgcagcc ccgctggtgg tcgtcgggtc ggttgcggcc
      121 ggcgtcctcg tttcgtcccg ggcgatgttc atcagcgacc acgactcgtc ggtcgtcctg
      181 ctcgtcgtcg cggccgtagc cccggtcgct ctggtctacg ggctcgtcct ggccagccgg
      241 gtccatcggc tcgatcgggc ggctgccgag cgggccgcgg aggccgtggc cgcccacgag
      301 cgggacgcag cggtggaagc ccagcgccga gacttggtcg cgtgggcctc ccacgactta
      361 cgcacccccc tggccgggat ccgcgccatg gccgaggcga tcgaggacgg agtcgctccc
      421 gccgagggtg cctacccggc ccgcatcaag gcccaggccg accggatggc cgcgatggtc
      481 gatgacctgc tcgcgctctc gcggatccac tccggcacca tgacgctcgc ccgcgaacag
      541 gtgagcgtgg ccgatctcgt gtccgacgcc ctggccacag gcgccgcctt ggccgagcgc
      601 tccggggtcc tcgtcgacgg agccgccgac ggcccggtcc ttgcccgagt cgatgcccgt
      661 gaactgggtc gggtgttgga caacctcatc gcgaacgccg tgcacgccac gccgcgcgga
      721 ggccgggtgg tggtccacgc cgccgcctcc cccacccagg tgaccattcg cgtcaccgac
      781 gagtgcggcg gcataccggc ggatgttcgg gcgcggatgt tcgagccgtg gtggcgcggg
      841 gacgacgccc ggaccccgcg cgccggcgac ggagcgggtc ttggcctggc cgtcgtccag
      901 gggatcgtgt ccgcccacgg aggcaccgtg ggtgtcgccg acgtcccggg tgggtgcgcg
      961 atcgaggtcg tcctcccgcg cgactgaccg ccgggcggtg ctcagccctc ggggatgaac
     1021 ccggagtcca actcccaggg ggcgatcggc gagtgttcga ggatccaggt ctgcagtgag
     1081 cggtcccaac cgagggcgat gccgatcccc acgaggatga agaccaggcc gaggatgcgc
     1141 cggaaccggc cgtgcgggtc ggccgcccag ccgagtcgcg cgatgaggcg gcgaccgagg
     1201 agcgcgattg ccaacagggt gacacagagc ccgagcacgt atgccgcgag cagcgtcaac
     1261 ccgtagccga accgagcggg caggacggtg acgacgacat aggcgtagag cgggctgcac
     1321 gaggagaaca ccgggccgag cgcagccccc gtgaggatgt cgccggtcaa acccccacgg
     1381 gcccgggcgg cgcggagccg ggcatccgag cgcccttgca ggctgagggt ggcggacagg
     1441 cgctcccaca ggtcggggaa ggcacccgcg atcccgagga gcacgaggat caccccggag
     1501 aggatcgacc aggtgcgggg atcgatgacg agcagcgccg tcgtggcctt gaggatgagg
     1561 gtgaagagcg tgatcgacgc gccgagtgag gccgtgatga ccagagcgcg gcggttgcgg
     1621 gtgacccaac ggacctgggt gcgcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnncgtgg aagaacagga cgaccttgga gccctggtac atcgccatcg acgactggta
     1801 gtcggcgtag gtgacgtagg cacccttggc catggccgcg ggggcaccgg tcttgtcggg
     1861 catcgccgtc ttgtcgggca tggcggtctt gtccggcatc gcggtcttgt ctgacatggc
     1921 tgtgctggcg gccggagcgg ccaacggggt ggcactgccg ccgcaggccg agagggtcag
     1981 ggcggtcccg agggccagcg ctgcagcggt gaactggggg cgggagaggg tgcgtgaagt
     2041 catgggacca gcctcacggc atacccccgc ggtggccatt gcgcagacct tacggggcgg
     2101 tgacgccgga cgtcagcggg ccagaccggg gatcgcgagg tagagcagcg ggtcgacggc
     2161 gaccgcgacg aacaggaccg tgagatacgt gatcgagtag tggaacaggc gcatggggcg
     2221 cagttgggcc tgcggcgcac cggcgagcgc ctggtggtgc agtcggtgcg cctcggcgat
     2281 gaacaggccg ccggagacca cagcgcccac ggcatacacc cagcccatgg gggccacggg
     2341 gatgagggtg agtgacacga gcaccatcac ccacgagtag atgacgatct ggcgcgagac
     2401 gacgaccatt cgcgccacca ccggcagcat cggcacagag gcggccgcgt agtcgtcctt
     2461 gaacttcatc gacagcggcc agtagtgcgg cggcgtccag aagaagatga cgaggaagag
     2521 gatccacgcc ggccagtcga cccggttggt gacggccgac cagccgatga gcaccggcat
     2581 acaccctgcg gcgccgcccc agacgatgtt ctgcggggtg cggcgcttga ggacgaccga
     2641 gtagatgccg acatagaacg cgaaggccgc gagggccagc caggccgaga gccagttgac
     2701 gaagactccg agcacgagcg tcgcgaccgc gcacaaggtg aacccgaaga tcacggcggc
     2761 gcgggtcgag atggtcccgg tgaccagggg gcggctggcg gtgcggttca tcagcgcgtc
     2821 gatgtcgcgg tcgatgacgc agttgagcgt gttggcggcg cccgcggaca acgtcccacc
     2881 gaccagggtg gcgaggacca gccaccccga cggtatgccg cgctcggcca ggaccatgac
     2941 ggggaccgtc gtgatgagca gcagctcgat gatccggggc ttggtgaggg cgacgtactg
     3001 gccgacgacg aagccgaagc cgcggcccgt ggaccccggg tgcgaggcga gctcggcgcg
     3061 cgggtcgacg gtggtcacag tcggggagtc taccggcggc atgacgcctg ctggcacggg
     3121 gccaaggtgg cgatccccac tggattaggc tgcacgtgac ccgtccagtg tgaaggagaa
     3181 cacctccgtg acatccgcct cgtccccttc caagctcttg gcaggtcgcg actcccacgt
     3241 gccccgcccg atggcgacgg cggtcgggtg gaccgatctg gacatccgag ctgtcgacac
     3301 ggcccggctg ttggcggccg atgcggtgca gaaggtcggc aacgggcatc cgggcacggc
     3361 gatctcactg gcccccgcgg catacctgct ctatcagcac gtgatgaccc acgatccgag
     3421 cgatccgcac tggctgggac gcgaccggtt cgtcctgtcc tgcgggcact cgagcctcac
     3481 gcagtatgtc cagttgttcc tgtcgggcta tggcttggag ctctcggatc tgcagtccct
     3541 gcgcacctgg ggcagcctca cgcctgggca tccggaggtc aaccacactc ccggcgtcga
     3601 gatcaccacc ggtccgttgg gttcgggctt ggccagcgcg gtgggcatgg cgatggcgca
     3661 acgccgccag cgcgggttgc tcgacccgga ggcggctgcg ggcgagagca tcttcgacca
     3721 ccacatctat gtcctggcct ccgacggcga catcatggaa ggcgtcaccc acgaggcgtg
     3781 cgcgctggcc gggcatcagg agttgggcaa cctcacggtc atctacgacc agaaccagat
     3841 ctccatcgag gacgacacgg acatctcgtt cagcgaggac gtcgcggcgc gttatgtcgc
     3901 ctacggctgg gacgtccgca cggtcgactg gcggggcgcg accgccgacg gggccaccta
     3961 tgtcgaggac atcgacgccc tcaacgcagc catcgaggcc ggccgcgcga tcaccgacaa
     4021 gccgagcctc atcgttctgc gcacgatcat cgcctggccc tcccccacca agcagaacac
     4081 cggcaagtcg cacggctcgg ccctgggcgc cgacgagatc gctgggctca agaccgctct
     4141 cggcttcgac ccggaggtcg ccttcccggt cgccccgcgc gtcctcaccc aggcgcgcaa
     4201 ggtcgtcgcc cgcggcaagg ccgcgcacaa ggcctgggac aagaggtatg ccgcgtggcg
     4261 caaggcccag cccgagcgcg ccgcgctgct ggaccggatc gtcgagcggg gcctgccgac
     4321 cgatctggcg gcggccctgc cgagttggcc ggccgacgcc aagggcatcg ccacccgcgc
     4381 cgcgtccggc aaggtgctct cggccctggc ccccgtgctg cccgagttgt ggggcgggtc
     4441 ggccgacctg gccgaatcga acaacacgac catggccggc gagccgtcgt tcatcccggc
     4501 ccgacgccag acgcacgagt ggaagggcgg cccctatggg cgcaccctgc acttcggcat
     4561 ccgtgagttc gggatgggcg cgatcctcaa cggcatcgcc ttggagggcc tcacccggcc
     4621 ctatggcggc accttcctcg tcttctccga ctacatgcgt ggtgccgtgc ggctggccgc
     4681 gatccagcag cttccggtga ccttcgtctg gacccacgac tcgatcggcc tgggcgagga
     4741 cggcccgacc caccagccca tcgagcacct ggcggctctg cgcgccatgc cggggctcga
     4801 cgtcgtgcgg cccgcggacg ccaacgagac ggcatacgcc tggttggaga tcctgcgcaa
     4861 cgcccggcct gcggcgctgg cgctgtcccg tcagaacctg cccgtcctgg accgcaaggt
     4921 ccacgcgccc gcccgcaatg tcgccaaggg tggctacgtc ctcatcgacg gcggggccga
     4981 tggccggcaa gccgccgagg tgatcctcgt ggccaccggc tccgaggtcg ccctggccct
     5041 ggccgcccgt gagcagcttg agcgggcccg gatccgtacc cgggtcgtct cgatgccctg
     5101 tcgtgaatgg ttcgaccagc agcccaagag ctaccgcgac cgggtcctgc cgccgaccgt
     5161 gcgggcccgc gtgtccgtcg aggcgggggt cgcccaaggg tggcgggaca tcgtcggcga
     5221 tgccgggcgg atcgtgtcga tcgagcactt cggtgcgtcg gccgatgctg cgacgctctt
     5281 ccgggagttc gggttcaccc ccgaggcgat cgtcgccgcg gccaaggcct cgctcaagac
     5341 cgcccgggcc ggcgcgcgca aggaaggcct gacatgaccg cagcgagcac cgacaacctg
     5401 gccgacctcg cggcggccgg tgtctcgatc tggctggatg acctgtcccg aagccgactc
     5461 acctccggca gtctggccgc cctcatccgg gaccagcacg tcagcggggt gacgaccaac
     5521 ccgtccatct tccaggcggc catcacttcg ggcaccgact acgacgagca gttggcgagc
     5581 ttggctgcgg ccggggccga cgccgatgcg gcgctcttcg ccatcaccac cgacgacgtc
     5641 cgcgccggct gcgacgtgtt cgcctcggtg gctagggaca ccaacggcat cgacggtcgg
     5701 gtctccatcg aggtcgaccc gaggctggcc caccagaccg ggcccaccat cgaacaggca
     5761 aaggccctcg ccgcggcggt cggtcgcgac aacgtctaca tcaagatccc ggccaccctc
     5821 gaggggctgc cggcgattgc cgcggtgttg gccgaaggga tcagcgtcaa cgtcacgctg
     5881 atcttcggcc tccagcgcta tcgggcggtg atgaacgcct tcgtcgaggg actggagcgc
     5941 gctcacgagg cgggccacga cctgcgcacg atccactcgg tcgcctcgtt cttcgtgtcg
     6001 cgggtcgaca ccgagatcga cggtcggctg gaggccatcg gcaccgacgc cgcgcgggct
     6061 ctcaagggcc aggccgccgt tgccaacgct cggttggcct atcaggccta cgaggaggtc
     6121 ttcacgaccc cgcggtggcg ttccctggcc gacgagggcg cccggcggca gcggccgctg
     6181 tgggcatcca ccggcgtcaa ggaccccgcc tatcccgaca cgctctacgt cgtggacctc
     6241 gtcgcccccg actgcgtcaa caccatgccg gagaagaccc tgcgcgctgt cgccgatcac
     6301 ggcatcgtgc gtggcgacac gatcaccggg gagtatgccg ccgcccaggg cgtcctcgat
     6361 gcggtcgagg cgcaaggcat ctcctacatc gaggtcgtcg aattgctcga acgcgaaggc
     6421 gtcgagaagt tcgagaaggc gtgggtctcc ctcctcgccg acgtcaccgg cgccctcgcc
     6481 cgtgcgtgac ctggcaacgc ggcatacacc tgggttgacg tatgccgcgt ccccgcgccc
     6541 cgcgcaggta ggttctcgcc catgagtccc gcccgcgtgt ctgccggagt caacccgttg
     6601 cgcgatcgca gggatcgacg gctcccaagg atcgccgggc cgtgtggcct ggtgctcttc
     6661 ggggtgaccg gcgacctggc tcgcaagaag ctcatgccgg ccatctacga cctggccaac
     6721 cgcggcctac tgcctccggg tttcagcctg gtcgggttcg ctcggcggga ctgggccgat
     6781 caggacttcg gccagatcgt ccacgactcg gtgcgcgacc acgcccgcac ccccttccgt
     6841 gaggacgtct ggaagtccct cgccgagggc atccgcttcg tgcccgggac cttcgacgac
     6901 gacgcggcct tcgacaccct cgccgccacc gttcacgagc tggagcaggc gcgcggcacc
     6961 ggcggcaacc tcgcgttcta cctgtcgatc ccgccggggt tcttcgccca ggtgtgccaa
     7021 caattggatc gctcggggct ggcccactcg gacggtcgat cgtggcgtcg agtggtcatc
     7081 gagaagccgt tcgggcacga tctcgcgtcg gcccgtgagc tcaacgcgat cgtcgagtcg
     7141 gtcttcccgg ccgactcggt cttccggatc gaccactatc tcggcaagga gacggtgcag
     7201 aacctcctgg cgctgcgctt cgccaaccag ttgttcgagc cggtctggaa cgccaactac
     7261 gtcgaccatg tgcagatcac catggccgag gacgtcggca tcggcgggcg ggccggctac
     7321 tacgacggca tcggcgcggc ccgcgacgtc atccagaacc acctgctgca actgctggcc
     7381 ctgaccgcga tggaggagcc ggtctccttc gatgcggcca gcctgcgcgc cgagaaggag
     7441 aaggtgctct cggctgtcgt ggtccccgcc aagatcgggg aagggacagc gcgcggccag
     7501 tatgccgccg gctggcaggg cggcgagttg gtccccggct acctcgagga ggaaggggtc
     7561 cgcaccgggt ccaccaccga gacgtatgcc gcggtccgcc tcgaggtcgc gacccgtcga
     7621 tgggccgggg tgccgttcta cctgcgcacc gggaaacggc tgggcaagcg ggtcaccgag
     7681 atcgcggtcg tcttcaagaa ggcgccgcac ctgccgttcg acacgaccgc caccgaggag
     7741 ttgggccaga acgccgtcgt catccgggtg cagcccgacg agggcatcac catgcggttc
     7801 ggcgccaagg tccccggagc ccagatggag gtgcgtgacg tgacgatgga cttcggttac
     7861 ggcagtgcct tcaccgagag ctcccccgag gcttatgagc gactcatcct tgatgtcttg
     7921 ctgggcgaac cacccctgtt cccccgtcat gaggaggtcg agttgtcctg gcagattctc
     7981 gacccgatcg aggcctggtg gcggcggcac ggcaagccgg acccgtatcc gtcgggcgga
     8041 tggggaccgg cgtcgagcga ccggatgctg gcccgcgacg gccgggtatg gaggaagcca
     8101 tgatcgtcga cctcccctcg acctcgaccg cggccatcgg caagcagttg gtccagctgc
     8161 gcaacgaggt cggggccctg gccctgggcc gggtcctcac cctcatcgtc gtcgtcgatg
     8221 acgatgctgc cgacgaggcc gtcgagatcg ccaccgaggc ctctcgccag cacccctgcc
     8281 gcatcatcgt cgtcatcagt ggctcccggc ggggagccaa ccgcatcgac ggtcagatcc
     8341 gggtcggtgg tgacgccggt gccagcgagg tcgtcattat gcggctgttc ggcaaactcg
     8401 ccgagcacgg tcagagcgtc gtcaccccgc tgttgctccc cgactccccc gtcgtggcct
     8461 ggtggccgag ggaggccccc agcgacctgc gccacgaccc catcgcggcg atggccacgc
     8521 ggcgggtgac cgactcggcg gagtgctcac acccacgggc catgctgacc cgcctggccg
     8581 agacctacca gcccggcgac accgacctgt cctggacccg aatcaccctg tggcggggtc
     8641 tgctggcagc ggccctcgac gggccgccct acgagtcggt gacggccgcg acggtggcgg
     8701 gagcccccga ctctccatcg accgacctgc tcgccgcctg gctcgcctgg gctttgcggg
     8761 tgcccgtcac ccgagaggac acccaagccg gcagcggtat ctgcgcggtc ggtctggagc
     8821 gtgccagcgg cgccatcgac ctggctcggc ccggcgatct cgtcgccaca atgacccagc
     8881 ctggccagcc tgatcgtcgg atcagcctgc gccggcgcgg cgacgccgaa tgcctggccg
     8941 acgaattgcg tcgcctggac gccgacgaga cctacgccga cgtgctgcgc aaggggttgc
     9001 ccgccttggg caccacgaag ggggccagcc gctcggcagc ggtgagcgag tcgaagcgag
     9061 aggctgaacg ggagcgtcgc aaccatgcgg cccaacttcg gacggccgcc gccgaggccg
     9121 gatccttgcc ctcaaccgtc gacacgacca ctcccgccaa gccgtccggg tccaccccga
     9181 cgaagccccg cgtcacccgg tcgacctcag ccaccgcgcg caccggccgg accacgaagg
     9241 agcagccgtc atgagcgagc accccaccaa cgcacccctc gtcgtcgtgc accccgacaa
     9301 gcagggcgtc tcggacgccg ccggggcacg gctgctgctg gccctgctcg acaccctctg
     9361 tgtcagggac gacgcccacg ttgtgctcac cggcggcagc ctcggctcag acatgctcgc
     9421 gtcggcaggc cgctcgccgt tggcccggct tgtcgactgg tcccgcgtcc acctgtggtg
     9481 gggcgacgag cgctaccttc ccgacggcga ccccgatcgc aacgagaccc agaaccgcgc
     9541 cgccctgctc gatcacctgc cgttggaccc ggcgaagatc catggcatgg ccggcccgga
     9601 tcgttcggag tcggcggagg ccagcgccgc ggcatacgcg acggagttgc gcacccacgg
     9661 cggcggcctg ttcgacgtcg tcctgctcgg aatgggaccg gacgggcacg tcgcctcgct
     9721 cttcccgcag cacccggccc agttggtgag tgacgcgatc gccgtcgcgg tccacgactc
     9781 ccccaaaccg ccgccggacc gggtgtccct caccttcgag tgcctcgccc gcagtcgcca
     9841 ggtgtggctc ctcgtcgctg gcgccgacaa ggcccccgcc gtatgccgtg ccctcacccc
     9901 cggcgcgcaa cgctgggacg tccctgcggc cggtgtgcgt ggcttggatg ccaccttgtg
     9961 gctcgttgat gtcgaggccg cctcggaact cccctcatga tcacggaccc cgctgtggag
    10021 ttcgtcggcg agttgttggc cacgggcggg acgacgaccg ggttcgatgt tcccgagtcg
    10081 gtcgtcactg ccctcggatc gggcaagcgg cctaaggtgc tcgtgaccgt gggtgggcac
    10141 tcgtggcaga cgagcatcgc cccgatgggc gggcgcttca tgctgggtgt cagcgccgcc
    10201 aaccgggccg cggcgggcct tgaggctggg cagacggtcc ccgtgacggt cgtgctcgac
    10261 accgcgccga gggtgatcga ggtgccgagc gatctggctg aggcgttggc ggccgcaccc
    10321 gacgccgctg ccggttgggc ccggctcagc tactcgcacc agcgcgccca cgttgaggcg
    10381 ttgttggctg cgaagaagcc agagactcgg gcctcccggc tgaccaagac cttggcgatg
    10441 ctcaccgcct gaccgcaggt gaaggcccct gctcccccaa aatccccaca cccgccgggg
    10501 atctgcgatc aggcaggccc ggattgaccc cagatcctca catccgccgg ggatctgcga
    10561 tcgggctgac ctactggatc aggccgcgct gacgcagggc gcccagcgcc tcgttgagga
    10621 tggcctggcc gtcggcgtcg gagcgccgct ccttcacata ggccaggtgc gtcttgtagg
    10681 gctccacctt gggcggagcc ggagggttct cgcggtcctg gccagcaggc aacccacagc
    10741 gggggcagtc ccagtgctcg ggcgccacca tgccgggctc ctgcgcgaac gacggccggg
    10801 tttcgtggcc gttcgtgcac caataggaga tgtgcacgcg gggagcagcc tcgccgcgct
    10861 cggcctcgcc catgggtccg gcgccaacac ggctgccacg gatcgcattt ccactcgcca
    10921 ttgtcgttcc cccgtctcag gccgcgacga aacgctcgac caggcccagc ccgacgactg
    10981 ccgtgaacca caagatggcc accgcgatcg tgaaccggtc aaggttgcgc tcggccacgg
    11041 tcgacccgcc cagtgagctg gacatgccgc cgccgaacat gtccgacagg cccccaccct
    11101 tgcccttgtg catcaggatg agcagggtca ggaacaggcc ggagagcacc agcaacgcct
    11161 gcaaggcgat cttcaccacg gtcacgagtt gcgcgcccac tttcgggtcg gtcggagtgc
    11221 cccgaggggc cgagggtcag aatacccgta gaccgcccag atcccgaacg gccccacgcc
    11281 ggcgcccctc gcacagggcc cccacgcgca ggtatgccgc ccgctcgggt gagcgcgcgg
    11341 catacctgga tcatgcggca tagcgtgaac tgctgaggtg accgcgaggt cagccggtga
    11401 cgtggtcctt gaaccggcag atcgcggcga actcggcggc gtcgagcgaa gcgccgccga
    11461 cgagggcgcc gtcgacgtcc tccttggcca tgatggccgc gacgttcgag gacttgaccg
    11521 agccgccgta gagcacgcgg acgccgtcag ccaggtcgcc ggagtagagc tcggcgagca
    11581 gggtgcggat cgccgcgcac acctcctgag cgtcctcggg cgtggccacc tcgccggtgc
    11641 cgatggccca gatgggctca taggcgatga cgatcgtgcg ggcctgctcg gcgggaatgc
    11701 cctcaaggtc gcgggtcagc tgagcgacga cgtgggcgac ctggccaccg gccttgcgga
    11761 cgtccaggcc ctcgccgcag cacaggatcg gagtgacgcc gtggcggtag gcagccttga
    11821 ccttgtcgtt gatgacggcg tcggtctcgg cgtggtattc acgccgctcg ctgtggccga
    11881 tcgtcacgta ggtgcagccg agcttggcga ggaacgcccc ggagatctcc ccggtgtagg
    11941 cacccgagtc gtgaaccgac aggtcctggg cgccgtagcg cacggacaac ttgtcgccct
    12001 cgacgagggt ctgcaccgaa cgcaggtcgg tgaagggcgg caggaccgcg acctcgaccg
    12061 caccgaagtc gtgcttgccg tcgcgaagcg tccagtcgag cttctggacc aggtgggtcg
    12121 cctgcaggtg gtcctggttc atcttccagt taccggcaag cagcggaatg cggctcataa
    12181 gtggtgcggc tccttcgtgg tcagccgttg aggatggcga tgccgggcag ttccttgccc
    12241 tcgaggtatt ccagggaggc gccgccaccg gtggagatgt gaccgaactg gtcgtcggtg
    12301 aagcccaact gccgaacggc tgcggcggag tcgccgccac cgacgacggt cagtgccccg
    12361 tcggccgtgg cctcgacgag cgccttcgcg acggccgccg tgcccgcggc atacggcgcc
    12421 atctcgaacg cgcccatcgg gccattccag aagaccgtct tcgcgtcgcg caccttgctc
    12481 gcgtagagct ccgcggagcg gggaccgatg tcgagcccca tccggtcagc cggtatgccg
    12541 tccgccggca ccacgtcgtg ctcggcgtcg gccgagaacg cggtcgcggc gacgacgtcg
    12601 accggcagga tgatctcgac gccggactcc ttggcccggg cgaggtaacc cttgcaggtc
    12661 tcgatgttgt ccttgtcgag caggctggaa ccgacctcgt agccctcggc ggcgaggaag
    12721 gtgaaggcca tgccgccgcc gatgagcagc cgatcggccg tcttcatgag gttctcgatg
    12781 accgcgagct tgtcggcgac cttcgcgccg ccgagcacca cggcatacgg acgggccggg
    12841 tcctcggtga ggcgcttgag cacctcaacc tcggccgcga cgagaccgcc catcgccgac
    12901 ggcagcagcc ggacgatgtc gtagacggag gcctgcttgc ggtggacgac gccgaagccg
    12961 tcggagacgt aagcgtcagc gagcgcggcg agctgggcgg caaaggcggc ccgctccccc
    13021 tcgtccttgc tcgtctcacc cgcgttgaag cggatgttct ccagcagggc gacgtcaccg
    13081 tcggccaacc ccgcgacaac ggcctgggcc gactcgccga cggtgtcggt ggcgaaggcc
    13141 accttctggc cgagcaattc gctcatccgg ccgacgaccg gggcgaggga gtacttgtct
    13201 tcaggcgcac ccttcggccg accgaggtgg gcgcacacga caacgcgtgc gcccgcctcg
    13261 gcgagggcct tgatggtcgg caccgaggcg cggatccggc cgtcgtcggt gatgttcttg
    13321 tcaccgtcga ggggaacgtt gagatccgag cggacgagaa cgcgcttgcc gcgcaggtcg
    13381 cccaatccgg caatggtctt cacggtgcca acctcctgga gtgtggtgtc tctggtggga
    13441 ctagcgggtc agagcttcga cccgacgagg gcgcagaggt ccacgaggcg gttgctgtag
    13501 ccccactcgt tgtcgtacca gccgagcacc ttgacctggt tgccgatgac cttggtcagc
    13561 ggggcgtcga agatgcacga ggccgggtcg gtctcgatgt ccttggacac gatcgggtcc
    13621 tcggtgtaga cgaggtaacc cttgagcggg ccggactcgg ccgcagcctt gacgatggcg
    13681 ttgacctcgg cgacggtcgt ctcgcgggcg gcctcgaagg tgaggtcggt tgccgagccg
    13741 gtcggtgtcg ggacgcgcat ggcgtagccg tcgagcttgc ccttgagctc gggcaggacg
    13801 agcgccacgg ccttggcggc accggtggtc gtcgggacca tgttgagagc ggcggcgcgg
    13861 gcgcgacgca ggtccttgtg cggggcgtcc tgcaggttct ggtcctgcgt gtaggcgtgg
    13921 atagtcgtca tgaggccctt gacgatgccg aggccgtcgt tgagcgcctt ggccatgggg
    13981 gccaggcagt tggtcgtgca cgacgcgttc gagatgaccg tgtgcagggc cgggtcgtac
    14041 ttgtcgtcgt tgacacccat gacgatggtg atgtcctcgt tggacgccgg cgccgagatg
    14101 atgaccttct tcgcgccgcc gtcgacgtgg gccttggcct tggtcgcgtc ggtgaagaag
    14161 ccggtggact cgatgacgat gtcagcaccg acgctggccc agtcgagctt ggccgggtcg
    14221 cgctcggccc aggcagcgaa ggtcttgccg ttggccgtga tcgaggcctc gtcgtaggtg
    14281 acctcgcccg ggaaacgccc gaggatcgag tcgtacttca acaggtgcgc gaggctcttg
    14341 ttgtcagtca ggtcgttgac agccacgacc tcgatgtcgg cgccggactc gacaacggcc
    14401 cggtagaagt tacggccgat gcggccgaag ccgttgatgc ctaccttgac ggtcactgtg
    14461 atctaaacct tccgactgga gtcgatggac acgcccccgg gctcacctcg acgaccagcg
    14521 gccgtcgtcg gcagcccgtg gtaccgcccc aaccctagtg ctcccagcgg cccgcggtct
    14581 aagcggtctc aagacgcaaa tcgaggtcag gacgtaggtc cttgccgggc cgtgacgcac
    14641 gccaccgcag ggtgagcagc gggtgtcgcg cagatgtcca gaaggtggcg cacggcatac
    14701 cccatggggt atgccgtgct gccgcgggtc agcgcgcggc ggctactccg cgtgcggcgg
    14761 cggcggccgg tgcggcgacg gtcactcgag catgtcgggg gtgaggctgg cctcggtgct
    14821 cgggatcccg agatcggcgg ccttcttgtc agccatcgcc aacagccgcc ggatccgtcc
    14881 cgcgaccgcg tccttggtca tcggcggcga cgccaactca ccgagctcct ccagggaggc
    14941 ctgcttgtgt tgcagacgca acgcaccggc ctccttgagg tggtcgggag cctcgtcgcc
    15001 gagaatctcc agggcacgct ccacccgggc tccggccgcg acggcggcgc gcgccgagcg
    15061 gcggaggttc gcgtcgtcga agttggccag ccggttggcc gtcgcgcgca cctcgcggcg
    15121 catccgacgc tcttcccacg ccagcacggc gtcgtgggca cccatcttgg tgagcatggc
    15181 cgcgatggcg tcaccgtcgc ggatgaccac tcggtcgacg ccgcgcacct cgcgggcctt
    15241 ggccgcaatg cccaagcgac gggcggcgcc gaccagggcc agcgctgcct cggggccggg
    15301 gcaggttact tcgagcgcgc tcgaccgacc cggctcggtc agggagccgt gggcgaggaa
    15361 ggccccgcgc caggcagctt cggcgtcgca ggcgctggag gagaccacct ttgggggcag
    15421 ccctcgcacg ggcctcccac gaccgtcgac gaggccggtc tgacgtgcca ggatcgcccc
    15481 gtcgtcgacg acgcgcagga cataccgact cccccgccgc aggccgccac cggacatgac
    15541 gacgacttcg gcgcgatggc catagagctc ggcgatatcc ctgcgcagcc gacggacggc
    15601 gttggcggtg tcgagctcag cttcgatgac gatctggccc ccgacgatgt gcaagcctcc
    15661 ggcgaagcgg atcgtggccg agacctcagc cttgcgacag cacggcttgg cgatctgcag
    15721 ccgggacagc tcgtccttca ctcgcgccgt catggccatt gatcgaacct gcccttgcct
    15781 cgtgtgtcac caatcggatc gacccgcccc tgggtgacga atgccgcgtc agcctagccg
    15841 atgggaggtt cgcgtgcggc tcaagatgaa catcgatcaa catctcgggc aacgggttga
    15901 cggggctgcg gcaccccgcg ctcgactcat cccatgaggt cccggtatgc cgcggcgaga
    15961 cggagcgcgt cgtgctggcc ggggcgcccg gtggcggcga tcggcgaagt caccaaccgc
    16021 gctcccaagc cactggcgac cgctgccacc tcgtcggcat cgtcgaggac gctcgggtcg
    16081 gccaggacga cgtcaaggcg caattcgggg gcatgccgcc ccaacgaggt gaggtggtcg
    16141 acagcccgga agcccttcgt ctcgccctgg tggacagcga gattgaggtt gaggacccgt
    16201 ttgccgctcg tccggaccag cgcatcgcgg acgtcgggca cgaggacatg gggaaggacc
    16261 gaggtgaacc acgacccggg cccgaggagg acccaatcgg cgtcgtcgat cgcggccagc
    16321 gcttcgggac agggctgggg ttgggcaggg cgcagccgaa tgctggacac ccggccgcgg
    16381 gtcgacgcca ctcggtgctg gccacagacg acggacatgt cgtcgggtcg atccgggtcg
    16441 gccccgacga cgtccgcctc gatctccaac ggctcgccgg ccatgggcag gacccgcccc
    16501 cgggagtcca acagtctcgc cacgaggtca aggccggcga cggtatcgcc caactggtcc
    16561 cagagggcga cgatcaacag gttgcccatc gcatgcccgc ccaacgggcc atccccaccg
    16621 aaccggtgtt ggagcacgtc gcgccagatc tgcccccacg gccggtcgtc gcagagggcg
    16681 gccagcgcca tccgcaggtc gcccggcggc aggacgttga actcgtcgcg cagccgaccg
    16741 gatgatcccc cgtcgtcggc caccgtgacc accgccgtca cccggtcggt caccagacgc
    16801 agcgccgcca acgacgccgc caaaccgtgc ccgcccccca acgccaccac ccggggaccg
    16861 ttcattcgcg gccgagatcg cgatgcacga cgaacgccac cacgcgctcc gggtcgaagc
    16921 gcgcggccaa cgcctcggtg acggcgaccg agcgatgctt accgcccgtg cagccgatcg
    16981 cgagggtgac gtaccgccgc ccctcgcgct gatagccggc cagcacgggg tcgaacagcg
    17041 cgatcgcccg gtcgaggaac tccgttgccc cggcctggcc aagcacgtag tcggcgacgt
    17101 cggtgtcgat tccggacttg gcccgcaact ccgggaccca gaacgggttc ggcaggaagc
    17161 gcatgtccag gacgaagtcc gcatcgaggg ggacgccgta cttgaagccg aacgacatga
    17221 ccgccaagcg cacccgggac ccgacggtgg cgtcgaggag caaggacacc ttggcgtgca
    17281 gttggtggac gttcagcccc gaggtgtcga cgatgacgtc cgcctccgac cgtaggtcgg
    17341 ccatgagagt gcgttcggcg gcgatcgcgt caaggagtcg cccgttgccc tgcaacggat
    17401 gtggtcgtcg cacgctctcg aaccggcgta ccaacgcctc gtccgtggca tcgaggaaga
    17461 gcaagaccgg tcgatgcgga ccggacgtga gttgttcgag caggtgcgcg tcctggccgg
    17521 acagccaccg ggtccgggca tcgatgacga cggccagccg cggcgacggg ccgccgtccg
    17581 ccggaggccg ggcccgctcg tgcaacgact cgaggtccgg cagcaactgc ggcgggaggt
    17641 tctcgaccac atgccacccg tggtcctcga gcgccttggc gaccgtggaa cgcccggccc
    17701 cgctcatgcc cgtgacgacg acgaggggcg ctgggcctcc gtcggccggc acggcccgca
    17761 gcccctccac aggcacatgt tcgggtgcgt gctcgtccat gctcggtcct cccgctcagt
    17821 cgaggatctc tccggtggtc acgttaacgc tcgcaggttt cgtcgcggtg tcgcgggcca
    17881 gcgccacgac gatcgccgtc gccagggacg gcccgatccc gggcacctcc tggatctctt
    17941 ccggcgtggc cgctcgcagc cgcttgacgg agccgaacct cgccaggagc gccgcgcgac
    18001 gcgacggacc aagaccgggt atgccgtcga gcaccgatgc ggtcatcgcc ttggcccggc
    18061 gctgtcggtg gaaggtgatg gcgaaccggt gggcttcgtc gcggacccgt tgcaggaggt
    18121 agagaccctc gctcgtgcgc gggaggatga tcgggaagtc ctgtccggga acccacacct
    18181 cctccagccg cttggccagt cccacgaggg ccaggtcggt gatccccagc tcccgcaagg
    18241 cggctgccgc ggcggtgacc tgtgggagtc ccccgtccac gacgacgagg ttgggtcggt
    18301 aggcgaactt gcgcgggcgc ccggtctgcg ggtcgaggcc ggggacgagc ggcatacccc
    18361 ctggttcacc ggacgacccc gacagggccg ccgcttcggc ggcctcctgt gcctcctgag
    18421 ccgcagccga ctcggcggcc accagtcggg agaaccggcg ggtgaggact tcgcgcatgg
    18481 cagcggtgtc gtcgaccgtg gcttcggcat ggagttgccc agcgggcccg tcatcgggct
    18541 gcgggtcggg gcctcccacg aggaagcgtc ggtattcgcc ggtgcgcgcc aaaccgtcct
    18601 cgaagacgac catcgaagcg acgatgttgg tgccttgcac gtgtgacacg tcgaagcatt
    18661 cgatccgcag tggcgcatcg cgaaggtcga gcgcctcctg caactcctgt agggccacgc
    18721 tgcgcgccgt gagatcaccg gcgcgactga gcttgtgtcg ggccagggac tgcgcggcgt
    18781 tgcgttcgac ggtctccatc agggctcgct tgtcaccccg gcgcgggacg cgcaggtcga
    18841 ccgctgcccc tcgccgggtg gcgagccacg acgcgaccgg gcccgggtcg gagggcagga
    18901 cggggacgag cacctcacgg ggaacggcgt cggggggctg gtcgccgtag acctgctgca
    18961 ggaggtgctc gacgaggtcg ggaaggtctt cgtgttcctt ctcgcccacc cacccgcgtt
    19021 ggccacggac tcgcccgccg cggacatgga agacctgtac ggccgcttcg agctcgtcgt
    19081 cgacgatggc gaatacgtcg gcgtcggtcg cgtcgcccag gaccacggcg ttcttctcca
    19141 gcgccttggt gagtgccacg aggtcgtccc gcagccgagc cgcccgctcg aactccaact
    19201 cggcggcggc gtcgtgcatg gcccgctcgg tgcgacggac gaagcgggtg gtgttgcccg
    19261 ccatgaagtc gcagaactcc tcggcgatgg cgcggtgttc gtcctcggtg acccgcccga
    19321 cgcagggtgc cgcgcacttg tcgaggtagc ccagcagaca ggggcgtccg ctctgggccg
    19381 cccgcttgaa cacgccggtg ctgcacgtgc ggatcgggaa cacgcgcagg agcaggtcga
    19441 gcgtctcgcg gatcgcccag gcatgcgagt agggcccgaa gtagcgggtg cccttgcgct
    19501 tggcgccgcg caggacctga gctcggggga actgctcccc caaggtgacc gcgaggaacg
    19561 ggtaggactt gtcgtcgcgg tatcggacgt tgaagcgcgg atcgaactcc ttgatccacg
    19621 cgtattccag ctgcagcgcc tcgacctcgt tgccgacgac ggtccactcg acgctggcgc
    19681 ccgtgcggac catggtcgcc gtgcgggggt gcagggcggc aatgtcctgg aagtaggacg
    19741 acaggcgtgg gcgcagcgac ttggccttgc cgacgtagat gacccgcccg gaggcgtccc
    19801 ggaaccgata gaccccgggg tcgagcggga tctggcccgg ggccgggcgg tacgtcgatg
    19861 ggtcggccac ggccccactg taggaggcgg gaccgacggc ccccctgcgg cgctcacgcg
    19921 cgggccatcg tcccccgatg ttcctgttct ggtcggctct ggtcgcggcc tccggccgcc
    19981 gactcagctc agggtcaagg agtcaccaga gacggtgacc gtcttcgcgg ccagggggcc
    20041 cttcgccggg ccgctcacga ccgctccggt tgcggcgtcg aacacgctgc cgtggcaggt
    20101 gcaggtgatc ttggcgctct cgatcttgct caccaggcag ccctcgtggg tgcacaccgc
    20161 gctgaacgcc ttgaaggtgc cggcggtcgg ctgggtgacg acgacctgtc cgacgacctt
    20221 gccaccgccg accgggatgt ccgccgcctt gagcgcggaa cccgcggctt tcgtcgtctg
    20281 cgcgggggcg gctgagctgc caccacaacc ggcaagggtg cccgccgtgg ccgccgcagc
    20341 gccgacagtt ccggcgacct gcaggatcga gcgacgactg ggttgagtgg ccatctgggg
    20401 tcctccaagg cttgcgtggg tggtcaggga ccaagcatcc ctggtatgcc gccccccggc
    20461 cacctgtccg ttggggcacc tgcgggtgcc ccaacggccc atcggcccca taacgacacg
    20521 ggaacaaccc gaagccggtt ggggacaccg ggcctccgga cccgtactct gcaccctagt
    20581 ccgcggtcgt cgcggagtcc ggaggat
//
LOCUS       DAORPW010000031        19972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_470_length_19972_cov_632.940051, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000031 DAORPW010000000
VERSION     DAORPW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 19972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19972
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_470_length_19972_cov_632.940051"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1260)
                     /locus_tag="PLB86_04375"
     CDS             complement(70..1260)
                     /locus_tag="PLB86_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HRC64405.1"
                     /translation="MDAICQVTGWHRDHARKALRQRLAGPAPVRAPRVPTFQYDQAVI
                     DALVTCWSVLDGPTGKRLRPGLPTLVPTMLAHQELTCPPQVIDQLLGMSAATIDRRLR
                     PYRTGLVATKGQSMTRPGSMLKSAIPMKTWHDWDDTRPGFLQIDLVGHDGGDNNGAFH
                     YTLDATDVATGWTESITVRTKGERIVSAGLEQLRLAFPFAVLGIHSDNGSEFINHHLL
                     RWCDTRQITFSRGRSNHSNDQAHIEQKNWSEVRRAVGYYRYDTTRELDLLNELWPLQS
                     ILDNLFLPQQKLATKTRNGAKVTKTYDQGATPYTRLTRDHPDALHPHDHAALHARLTE
                     INPAGLRRQISAIQATLIELARRRGKVEQRPKANATYLSRRKMTTPKRATTDESTTHN
                     TRAS"
     gene            1504..1576
                     /locus_tag="PLB86_04380"
     tRNA            1504..1576
                     /locus_tag="PLB86_04380"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1537..1539,aa:Thr,seq:ggt)
     gene            1606..1679
                     /locus_tag="PLB86_04385"
     tRNA            1606..1679
                     /locus_tag="PLB86_04385"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1640..1642,aa:Met,seq:cat)
     gene            1838..2008
                     /gene="rpmG"
                     /locus_tag="PLB86_04390"
     CDS             1838..2008
                     /gene="rpmG"
                     /locus_tag="PLB86_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRC64406.1"
                     /translation="MASKTSDVRPKITMACTECKDRNYITKKNRRNDPDRLALAKFCP
                     KCGKHTEHRETR"
     gene            2520..2963
                     /locus_tag="PLB86_04395"
     CDS             2520..2963
                     /locus_tag="PLB86_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRC64407.1"
                     /translation="MPVNPEFAGRVYPPSAPYGVSAAKIKEFAEAVGSTDPVHTDRAV
                     AQARGYRDVIAPPTFAVLIAQRAEMEFIFDPEAGVDFSRVVHGEETFVHHRPLTAGDE
                     IVGQLTVDSVKAAGGHTMIVTRAELSAGDEPVCTALSTIVVRGGE"
     gene            2963..3412
                     /locus_tag="PLB86_04400"
     CDS             2963..3412
                     /locus_tag="PLB86_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRC64408.1"
                     /translation="MSAATMGAGSRREFSAVSVGEVLPAKTVHIDRARLVQYAGASLD
                     RNPIHWDERFAVKVGLPNVIAHGMFTMGAAVTVVTDWAGDAGAVVRYGTRFTKPVVVP
                     YETGADVVFSATVTALDEATRQATVEISALCGTDKVLGRATALVQLA"
     gene            3414..4487
                     /locus_tag="PLB86_04405"
     CDS             3414..4487
                     /locus_tag="PLB86_04405"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRC64409.1"
                     /translation="MQEAFAAPLAALTTMRVGGPADRLVTAESIDDIVDAVREVDDAD
                     EPLLLLSGGSNLVVADDGFRGTVLRIASRGVRVESLDRCSGATVRVAAGEPWDDLVVR
                     AVTEGWSGIEALSGIPGCVGSTPVQNVGAYGQEVADTIAQVRVWDRDREAVRTLTASE
                     CGFAYRDSVFKHRGARLVVLDVVFQLRLADLSAPVGYADLAAALEVAEGERVPLADAR
                     EAVLAQRRRRGMVLDAADHDTWSCGSFFTNPVLSESDYQTLVDRVADRLGPAGPAVPR
                     WSAPGGVKTSAAWLIERAGFGKGFGMPGPAALSTKHTLAVTNRGSATAAEIVALARTV
                     RDGVQDAFGVALVNEPVLVGLSL"
     gene            complement(4497..5243)
                     /locus_tag="PLB86_04410"
     CDS             complement(4497..5243)
                     /locus_tag="PLB86_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64410.1"
                     /translation="MPRRRATTAAPVVVTVLTVGLLVGGCSTGSPTAAPTTATTANAT
                     ATVTESATATSTDSSAATATATSTATDPAAPVPTARTATPPPAPTATAPPLTAALPTE
                     FTPMGQVIKDDSLGHEITVLRMARGLPWPVGSSGQSAAFELLALELRLTPGTKYTATL
                     RELDFALITGSQYPNRPDPLIDAQLALAGWALLPTEVPAGQNATGWVVFKVDPKAATS
                     IRLDYTRPEISVAGSRTRFPKKVFSLQIVG"
     gene            complement(5298..6311)
                     /locus_tag="PLB86_04415"
     CDS             complement(5298..6311)
                     /locus_tag="PLB86_04415"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRC64411.1"
                     /translation="MTTLPKAHLHLHFTGSMRIDTLLDLAVARGVRLPDALTDNWPPH
                     LHATDERGWFRFQRLYDLARSCVRGEEAMRRVVAEAAADDAAEGSRWLELQVEPSSYA
                     PHVGGLTPALEIVLDAAREASIDSGIGVGIVVAASRIRHPLDARTLARLAARYAGDGP
                     GTVVGFGLSNDERRGATADFAPAFDIARRAGLALVPHGGELLGPTSVQETLGALRPDR
                     LGHGVRSVEDPRVLDEVAEAGVALEVCPGSNLAMGVYATPREVPLLELVGHGIPVALG
                     ADDPLLFGSRLVDQYTLARDALGCSDRQLADLARDSLRASRAPEQVRLTALRDVDAWL
                     ARG"
     gene            complement(6344..6877)
                     /locus_tag="PLB86_04420"
     CDS             complement(6344..6877)
                     /locus_tag="PLB86_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HRC64412.1"
                     /translation="MSTDEAAFDNDTIGDGLEVWSVDDEDQLQQGDTLIDRGVDDVLD
                     EGFSPPDRPRGSYAYGTTAAEQRGHETIDQRILQEEPDPYSAYGAPDDESGEQRGRKE
                     KIGGDDPDSIYADADYVGQAGRVRSGRIVASAEDGNTEFENDVFAGDVGIDGGGASAE
                     EAAMHIIDLDSDEDLED"
     gene            complement(6964..8412)
                     /locus_tag="PLB86_04425"
     CDS             complement(6964..8412)
                     /locus_tag="PLB86_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64413.1"
                     /translation="MPSDPRGLRGDAPALDAWLAFQGGAPTPFTIPGHKQRLDLVGDV
                     VAGDVPLFAGLDTMSQAHGVLAAAEARAARLWGADVCRFSVGGSTHGNQALALAVGAP
                     GDRVIVSRTLHRSLLLGLVLAGLTPVWVRPDVDDLGMPRGIPPRALTDALALTPDAVA
                     VFIGDPSYVGTLTDIEGLAAVAHAHRGSRARGGADGIPLVVDAAWGAHFGFHPALPGH
                     ALARGADALVTSAHKTLPAWSQGALVLARTERIDPARLAAAVDATATTSPSGAILASI
                     DASRALVERDGPALLGDLLGQVADLRARLRAVPGLVVLDGPGVDPVKVCLVLSGTGAD
                     GTQIEADLIARRMPVELADRDTIVAMVTLADTPTTLRRLEDALVELVEVHRGTPRPTA
                     ASIAWSVAPQQVCPPRAAFFARQETVAAARAVGRTCGELVAPYPPGIPVLAPGELITE
                     ETMSALQAARAAGTRIAYAADPTLATVRVLAD"
     gene            complement(8454..9680)
                     /locus_tag="PLB86_04430"
     CDS             complement(8454..9680)
                     /locus_tag="PLB86_04430"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRC64414.1"
                     /translation="MTPVNDLIVPRRVSQRIGAIAESATLAVDAKAKALKAQGRPVIG
                     FGAGEPDFPTPDYIVEAAIAAARNPANHRYTPAAGLPELREAIAAKTLRDSGYAVPAT
                     AVLVTNGGKQAVYQAFASIVDPGDEVLLPAPYWTTYPEAIALAGGRAVEVFAGEDQGY
                     LVTVEQLEAARTENTKALLFCSPSNPTGAVYSPEQVRAIGRWAHDHGIWVITDEIYEH
                     LTYDGVRAASIVVEVPELADTCIILNGVAKTYAMTGWRVGWMIGPKDVIAAATNLQSH
                     LTSNVNNVAQRAAITALSGDLAAVDAMKVAFDRRRRTMVAMLNEIDGVTCPTPSGAFY
                     VYPSVKGVLGQTIRGARPLTSAELASLILDEVEVAVVPGEAFGPSGYLRLSYALGDDD
                     LVEGVTRIQSLLAEAH"
     gene            9888..9960
                     /locus_tag="PLB86_04435"
     tRNA            9888..9960
                     /locus_tag="PLB86_04435"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9921..9923,aa:Trp,seq:cca)
     gene            9996..10265
                     /gene="secE"
                     /locus_tag="PLB86_04440"
     CDS             9996..10265
                     /gene="secE"
                     /locus_tag="PLB86_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRC64415.1"
                     /translation="MSESSTARTGSRAVRQRPGDGNPFSRFFAALTLFIRQILDELRK
                     VVRPTGSELLNYTIVVILFVVTIMAIVAGLDVVLHRLVLWVFAGS"
     gene            10346..11146
                     /gene="nusG"
                     /locus_tag="PLB86_04445"
     CDS             10346..11146
                     /gene="nusG"
                     /locus_tag="PLB86_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRC64416.1"
                     /translation="MADQDEVLDIEADETHLDDVEVVEVDGADADLVVDEADADDTAD
                     ADDTADADEAESSEEAAEVIDPVAEFKATLRAKPGEWFVVHSYAGYENRVKANLETRI
                     ISLNMEDYIFEIDVPMEEVTEIKNGQKKLVRRVRMPGYVLVRMDLTDASWGAVRHTPG
                     VTGFVGNAHQPIPLTLDEVYRMLAPDLAPAAGPAGAGAVGTGSKPKEVRVVDFEIGES
                     VTVMEGPFETLPATISEINPDTQKLKVLVSIFGRETPVEVSFGQVAKI"
     gene            11235..11672
                     /gene="rplK"
                     /locus_tag="PLB86_04450"
     CDS             11235..11672
                     /gene="rplK"
                     /locus_tag="PLB86_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010058817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRC64417.1"
                     /translation="MPPKKKKITGFIKLQINAGAATPAPPVGPALGQHGVNIMEFVKA
                     YNAATEHQRGNVIPVEITVYEDRSFTFVTKTPPAAELIKKAAGIAKGSSEPHKTKVAK
                     LTADQVREIAQQKLEDLNANDVEMAARIIAGTARSMGVQTEGY"
     gene            11788..12501
                     /gene="rplA"
                     /locus_tag="PLB86_04455"
     CDS             11788..12501
                     /gene="rplA"
                     /locus_tag="PLB86_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRC64418.1"
                     /translation="MKRSKAYRAAVDKMTPGKVYGPLEAVRLAKATSATKYDATVEVA
                     LRLGVDPRKADQMVRGTVNLPNGTGKTARVLVFANADKAEAARAAGADFVGADDLIEK
                     ITGGWLDFDAVVATPDMMGKVGRLGKVLGPRGLMPNPKTGTVTMDVAKAVTDIKGGKI
                     EFRVDKHGNLHFIIGKTSFDERALVENYSAAIDEVLRLKPSASKGRYIEKATMATTMG
                     PGIPLDFTKTRNLLAEDEA"
     gene            12640..13401
                     /locus_tag="PLB86_04460"
     CDS             12640..13401
                     /locus_tag="PLB86_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64419.1"
                     /translation="MKSVLMCATVLVAAVSLAGCGGTAAPAVATATTTPAASTSAAST
                     APTTNAPPAADGIDGTGIAAKMVMALVKAGSGRYDMVSSGAAGGQQVTMTAKSEFVVV
                     NGAMDSKATMSVAGGSMEVVSVGGVMYMKGLIKSKDGKPWVKIDPNGTDEFSKRMAPQ
                     LKSAADLRSQVEVYEGSKATLIGTDAGVRHYRLTGVSSAPAQPFDIYLDASDLPVKVT
                     TKSDSMEMTITYSGFGDAVTVTAPPAAQVGPPPAM"
     gene            13570..14133
                     /gene="ahpC"
                     /locus_tag="PLB86_04465"
     CDS             13570..14133
                     /gene="ahpC"
                     /locus_tag="PLB86_04465"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HRC64420.1"
                     /translation="MALIGSEIKPFTATAFKSGSFVVVSEHDLKGKWSVVFFYPADFT
                     FVCPTELGDMADHYAELQAMGVEVFSVSTDTHFTHKAWHDSSDTIGKIDYFMVGDPTG
                     EITTNFKVMREGQGLADRGTFVVDPDGIIQVMEVTCEGVGRNAAELVRKVKAAQYVRN
                     HPGEVCPAKWEEGAETLAPSLDLVGKI"
     gene            14265..15845
                     /gene="ahpF"
                     /locus_tag="PLB86_04470"
     CDS             14265..15845
                     /gene="ahpF"
                     /locus_tag="PLB86_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HRC64421.1"
                     /translation="MLDPNLTAALKSYLERLTLPVELEASLDDSRASAQTRELLGEIA
                     ALSALVTVTSGDDARKPSFAVKRAGTDIQVRFAGVPLGHEFTSLVLALLQVGGVAPKI
                     SEETAAAIRAIEGEHHFETYMSLTCQNCPDVVQALNAMSVLNPRITHVTIEGGAFQGE
                     IESRKVLAVPTVYRNGELFGQGRMTLEEIVGKLDSGAAARDAQRLEAMDPFDVLVVGG
                     GPAGAAAAIYASRKGIRTGIVAERFGGQVMDTMAIENFISVTHTEGPKLAAALEEHVR
                     SYEVEVMELQRAAALTPAAEPGGLAQVRLDNGAVLSARTVILATGARWRHMGVPGEED
                     YRNKGVTFCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLAGVVGHVTVLEFMDELRADA
                     VLQRKLYSLPNVEVILSAKTTEVLGNGEQVTGLDYEDRTTGASRHLDLEGIFVQIGLL
                     PNTEWLADALTLTPRKEIVIDDRGQTSATGIFAAGDCTTTPYKQIVISLGAGATAALG
                     AFDHLIRSTAPEASLVDA"
     gene            15901..16809
                     /locus_tag="PLB86_04475"
     CDS             15901..16809
                     /locus_tag="PLB86_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRC64422.1"
                     /translation="MNLRDLQYLVAVADHRHFGRAATACAVSQPTLSTQLKKLEVELG
                     VQLVERGGRAVILTPVGDQVVARARAVLAEGEAIRRIARQAQDPRAGRLALGAFPTIA
                     PYLFPHVLGELRRQLPDVELLLVEDKTAALLTQLREGSLDAVVVALPVDEEGLHAEPL
                     FREALLLAAPATHPLGRDTSPVGLEDLAGESVLLLSRGHCLREQALAVCRAAGAVERP
                     GFRATSLDTLRHMVEAGLGLTILPRLSLPEARELPPEVVVREFREPTPYRDLALVWRL
                     GSVRDALLPEVAQLLRRLPTGLVTPL"
     gene            16890..17372
                     /locus_tag="PLB86_04480"
     CDS             16890..17372
                     /locus_tag="PLB86_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRC64423.1"
                     /translation="MPAHTEVARALRDAGLRVTQPRLAVLAAVHDHPHSDTEGLIVAA
                     RAELAGVSHQAVYDVLRALTDAGLVRRIQPPGSIARYEARVGDNHHHLVCRHCASIVD
                     VDCAVGERPCLTASDDHGYVIDEAEVVYWGTCPACLAGSPPPSSSDTRSTHHPEGSEP
                     "
     gene            17369..19603
                     /gene="katG"
                     /locus_tag="PLB86_04485"
     CDS             17369..19603
                     /gene="katG"
                     /locus_tag="PLB86_04485"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012880052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HRC64424.1"
                     /translation="MTDHDAAVGDPTSDLSEASCPVAHARPQHPTEGSGNRTWWPNQL
                     NLRILATNPPAGNPMAAGFDYAAAFGALDLPSVKRDIESVLTTSQPWWPADYGHYGPL
                     MIRMAWHSAGTYRVSDGRGGAGAGQQRFAPLNSWPDNANLDKARRVLWPVKKKYGRAL
                     SWADLMILAGNVALESMGFTTAGFGGGRADVWQADEEVYWGPEDTWLGDERYTGDRDL
                     ENPLAAVQMGLIYVNPEGPNGNADPLASARDIRETFARMAMNDEETVALTAGGHTFGK
                     AHGADDPNRYLGPEPEGADLAAQGLGWLNSKNAGNGVDTITSGLEGAWTPTPTTWDNG
                     FFETLFRYDWELTRSPAGAQQWRPKGGAGASDVPDAHDPTVRHAPMMTTADMAMRVDP
                     VYEQISRRFLEHPDEFADAFARAWFKLTHRDMGPIERYVGSEVPAERFIWQDPVPAVT
                     HPLISDADAADLKRTILASGLSVPALVGTAWASAATFRGGDKRGGTNGARIRLEPQNG
                     WDINEPERLASVLTTLGGIQETFNADHPAGPHVSMADLIVLGGNAAVEQAARNAGVEV
                     TVPFRAGRGDAVQEMTDVESFEVLKPAADGFRNYAGKGLRLPAEYHLIDRANLLTLSA
                     PEMTVLVGGLRVLGANYQGSRLGVLTDRTDTLSNDFFTNLLDLGIHWTPTDEAQDTFE
                     GRDRATGEVRWTGSRVDLVFGSNSELRALAEVYASDDAQASFVHDFVAAWDKVMNLGR
                     YGRD"
BASE COUNT         3109 a         6791 c         6953 g         3119 t
ORIGIN      
        1 gcaggccgag tgggacggcg atccagagca ccgggccact ctagagccaa ccgatcatgt
       61 tgactcatgt caagatgccc gcgtgttgtg ggtcgttgac tcatcggtag ttgcccgctt
      121 gggggtggtc attttccgtc tgctcaggta ggtggcgttg gctttgggtc gttgttcgac
      181 tttgccgcgg cggcgggcta gttcgatcag ggtggcttgg atggcgctga tctgtcggcg
      241 gaggccggct gggttgattt cggtgagtcg ggcgtgcagg gcggcgtggt cgtgggggtg
      301 cagggcgtcg gggtggtcgc gggtgagtct ggtgtagggg gtcgcgccct ggtcgtaggt
      361 tttggtgact ttggcgccgt tgcgggtctt ggtggcgagt ttctgttggg ggaggaacag
      421 gttgtccagg atcgactgca ggggccacag ttcgttgagt aggtcgagtt ctcgggtggt
      481 gtcgtagcgg tagtagccga cggctcggcg gacctcggac cagttcttct gttcgatgtg
      541 ggcttggtcg ttgctgtggt tggagcggcc gcggctgaag gtgatctgac gggtgtcgca
      601 ccagcgcagg aggtggtggt tgatgaactc ggacccgttg tcgctgtgga tgcccaggac
      661 cgcgaacggg aacgccagtc gtagttgttc caggccggcg gagacgatgc gttctccctt
      721 ggtgcgcacc gtgattgatt cggtccatcc ggtggcgacg tcggtggcgt ccagggtgta
      781 gtggaacgcg ccgttgttgt cgccgccgtc gtggccgacc aggtcgattt gcaggaaccc
      841 ggggcgggtg tcgtcccagt cgtgccaggt cttcatgggg atcgcggact tgagcatgct
      901 gccggggcgg gtcatcgact ggcctttggt ggccaccaga ccggtccggt agggacgcag
      961 ccgccggtcg atggtggccg cggacatgcc cagcaactgg tcgatcacct gcggggggca
     1021 ggtcagttcc tgatgggcca gcatggttgg taccagtgtc ggcaggcctg ggcgtagtcg
     1081 tttgccggtg gggccgtcca gcacgctcca acaagtcacc agggcgtcga tgaccgcctg
     1141 gtcgtattgg aaggtcggca cccggggggc ccgtaccggt gccgggccgg ccaaccgttg
     1201 acgtaacgcc ttacgggcat ggtcgcggtg ccagccggtg acctggcaga tcgcgtccaa
     1261 cagggccgac ttctcggcct tcgtgccagt gcgataccga tcgacctgcg ccttggtgat
     1321 ctgtcgccgt gtcgccaacg tgagctccat ccgtcatcgt gacccacgcg ggcatccttg
     1381 gacgagtcaa cgacccccat cacgcgggca acttggatga gtcaactcgc cgatttctac
     1441 tgtggccacg cgagcgtgta ctctctggac cgcactcctg gggccccttg ggcggccagg
     1501 ggcgccccaa tagctcagtc ggcagagcgt ttccatggta aggaaaaggt ctagggttcg
     1561 attccctatt ggggctcggg atcaggcgtc gcgccggatc gccacggcgg gatagctcag
     1621 ttggtcagag cgcacgactc ataatcgtga ggtcgcggga tcgagccccg ctcccgctac
     1681 tgcttccgat cggggccgcc cagcgtccgg tcggaccgcg gatttcccgg ttcgacgccc
     1741 gatcacgtac ccttgagcgt tgcgcgcgcg cagccgagtg tttgcgcccg cgtccgacag
     1801 accagcccat cgacagacca gcagcgaggt agccaccgtg gccagcaaga cgagcgatgt
     1861 ccggccgaag atcaccatgg cgtgcaccga gtgcaaggac cgcaactaca tcaccaagaa
     1921 gaaccgccgc aacgaccccg accggttggc gctggccaag ttctgcccca agtgcggcaa
     1981 gcacacggag caccgcgaga cccgctaagg ctcgcagccg gcatacgtca aggccgcgtc
     2041 tcccctgggg agccgcggcc tttgtcgtgc ccgggagcgg ggggaccggc gcgaccgacc
     2101 gggcgacgcc ccgacccggc ggcgcgggtg ccccagatca cgggacgtcg gcccttccgt
     2161 cggaggcgac acgctgctaa tgtctccggt ttgcttcacc caagatgaac tgctaacctt
     2221 acgaaggtga acaacgggtg aacggccggt gaggtctaaa ccccagcgca ccacgagccg
     2281 ccaccacgag ggtgacgacg tgacattccc aaggggggat tgacatgcgc aaactctttt
     2341 ctctgtaccg cagcgactac aagtcgacgc cgtgcatcgc gagcctggcc gtgcttccgc
     2401 tgaagcccga ggcctgagcc gactaatggt cgaagccccc gtcgcctggc gacgggggct
     2461 tcgccgttgt caggcttgcg acggcgccgt cgcgtcgagg gtggcctagg cttccccgta
     2521 tgccggtcaa tcccgagttc gcgggacggg tctacccgcc cagcgccccc tacggcgtca
     2581 gcgccgccaa gatcaaggag ttcgcggagg cggtgggctc gaccgatccg gtgcacacgg
     2641 atcgggctgt cgcccaggcc cgcggctacc gcgacgtcat cgccccgccg acgtttgcgg
     2701 tgctcatcgc gcagcgcgcc gagatggagt tcatcttcga tcccgaggct ggtgtcgact
     2761 tcagccgggt cgtccacggc gaggagacct tcgttcatca ccgccccctg accgccggtg
     2821 acgagatcgt cgggcaactc accgtcgact cggtcaaggc ggccggtgga cacacgatga
     2881 tcgtcacccg cgccgagttg tcggccggag acgagcccgt gtgcacggcc ctgtccacga
     2941 tcgttgtcag gggaggcgag tgatgtccgc tgccaccatg ggggccggtt cgcgccggga
     3001 gttctccgcc gtctcggtcg gcgaggtcct gcccgccaag accgtgcaca tcgaccgggc
     3061 ccggctagtg cagtatgccg gcgcctccct cgaccgtaac ccgatccact gggacgagcg
     3121 gttcgccgtc aaggtcggtc tgccgaacgt catcgcccac ggaatgttca ccatgggcgc
     3181 agcggtcacg gtcgttaccg actgggccgg agatgctgga gcggtcgtgc gttacggcac
     3241 ccggttcacc aagcccgtcg tcgtgcccta cgagaccggt gcagacgtcg tgttctcggc
     3301 gacggtcacc gcgctggacg aagcgacccg ccaagcgacc gtcgagatct ccgcgctgtg
     3361 cgggaccgac aaggtgctcg gtcgggccac ggcgctcgtc caactcgcct gacgtgcagg
     3421 aggctttcgc agcgccgctc gcggcgttga ccaccatgcg ggtgggcggg cctgctgatc
     3481 gcctcgtcac ggccgagtcg atcgacgaca tcgtcgatgc cgtccgcgag gtcgacgacg
     3541 ccgacgagcc gctgctgctg ttgtccggcg gctcgaacct cgtcgtggcc gacgacggct
     3601 tccgcggcac ggtgctgcgg atcgccagtc ggggcgtgcg ggtcgagtcg ctcgaccggt
     3661 gcagcggcgc gacggtgcgg gtcgcggcgg gcgagccgtg ggacgacctg gtcgtccggg
     3721 ccgtcaccga gggctggtcg gggatcgagg cgctgtccgg gatcccgggg tgcgtcgggt
     3781 cgacgccggt acagaacgtc ggggcctatg gccaagaggt tgccgacacc atcgcccagg
     3841 tgcgcgtctg ggaccgggac cgtgaagcgg tgcggaccct gaccgcctcc gagtgtgggt
     3901 tcgcctatcg cgactcggtc ttcaaacacc gaggcgcccg gttggtcgtc ctcgatgtgg
     3961 tcttccaact gcgcctggcc gacctgtcgg cgccggtggg gtatgccgat ctcgcggcgg
     4021 cgttggaggt tgccgagggc gagcgggtgc cgttggccga tgcacgtgag gcggttctgg
     4081 cccagcggcg gcgccgtggc atggtcctcg acgccgctga ccacgacacc tggagttgtg
     4141 ggtccttctt caccaatccg gtgctctccg agtccgacta ccagaccctg gtcgatcggg
     4201 tcgcggaccg gctgggaccg gcggggccgg cggtgccgcg gtggagcgcc cccggaggcg
     4261 tcaagaccag tgcggcctgg ctgattgagc gggccggctt cgggaagggc ttcggtatgc
     4321 cgggcccggc tgccctgtcg accaagcaca ctctcgcggt gaccaaccgc ggcagcgcga
     4381 ccgccgcgga gatcgtcgcc ttggcgcgca ccgtccgcga tggggtgcag gacgcgttcg
     4441 gggtggcgct ggtcaacgag cccgtgctcg tcgggctcag cctctgactg cgccggtcac
     4501 ccgacgatct gcaggctgaa gaccttctta gggaatctcg tcctgctccc ggcgaccgag
     4561 atctccgggc gggtgtagtc caaccggatc gacgtcgcag ccttggggtc gaccttgaag
     4621 acgacccacc cggtggcgtt ctggccagcc gggacctcag tcggcagcag ggcccacccg
     4681 gcgagcgcca gttgtgcgtc gatgagaggg tcgggccgat tcgggtactg cgatccggtg
     4741 atgagggcga agtccagttc gcgcagcgtc gcggtgtact tcgtgccggg tgtcaatcgc
     4801 agctccaacg ccaacaactc gaacgccgca gactgccccg aggaccccac cggccagggc
     4861 aggccgcgag ccatccgcag cacggtgatc tcgtggccga gcgagtcgtc tttgatcact
     4921 tgacccatcg gcgtgaactc ggtcggcagg gcggccgtga ggggaggcgc ggtcgcggtc
     4981 ggtgcgggtg ggggagtggc tgttcgtgct gtcgggactg gggccgccgg gtcggtcgcc
     5041 gtgctcgtcg cggtcgccgt cgcagcgctc gaatcggtcg aggtggctgt cgcgctctcg
     5101 gtgacggtcg ccgttgcgtt agcggtggtg gcggtggttg gcgccgctgt cggtgacccg
     5161 gtcgaacaac cgccgacgag caggccgacg gtcaggacgg tcaccacgac gggggcggcg
     5221 gtggtggctc ggcggcgcgg catactcctc acctgcacca gcctaaacgg ctccgggagt
     5281 gcgccccgac gggtcggtca cccgcgagcc agccacgcat cgacgtcgcg caacgccgtg
     5341 agtcgcacct gctcaggcgc gcggctggcg cgcagcgagt cgcgggcgag gtcggccagt
     5401 tgccggtccg agcaccccag ggcgtcccgg gcgagggtgt attgatcgac caggcgcgac
     5461 ccgaagagca agggatcgtc ggcccccaga gccacgggta tgccgtgtcc caccaactcg
     5521 agcaggggca cctcacgggg cgtcgcgtag acccccatcg cgaggttgct ccctgggcag
     5581 acttccagcg cgaccccggc ctcggcgacc tcgtccagca cccgagggtc ctcgaccgat
     5641 cgcaccccat gaccgagtcg atccggtcgg agtgcgccga gggtctcctg aacggacgtc
     5701 ggacccagca gctctcctcc gtgcggcacc agggccagcc ccgctcgccg cgcgatgtcg
     5761 aaggccggtg cgaaatccgc tgtcgcgccg cgccgttcgt cgttggacag cccgaacccg
     5821 acgaccgtcc cggggccgtc gccggcatac cgggcggcga gccgggcgag ggtgcgggcg
     5881 tcgagcggat ggcggatccg cgaggccgcg acgacgatcc cgacgccgat gccggagtcg
     5941 atcgacgcct cgcgggccgc gtcaaggacg atctccaagg cgggcgtgag gcccccgaca
     6001 tgcggcgcat aggacgacgg ctcgacctgc agttccagcc agcgggagcc ctcggcggcg
     6061 tcgtccgcgg cggcttcggc aaccacccga cgcatcgcct cctcgccccg cacgcacgag
     6121 cgggccaggt catagaggcg ttggaagcga aaccacccgc gctcgtcggt ggcgtgcaga
     6181 tgcgggggcc agttgtcggt cagcgcgtcg ggcagccgca ccccgcgggc gaccgcgagg
     6241 tccagaagtg tgtcgatccg catcgatccg gtgaaatgca ggtgcagatg agccttgggg
     6301 agggtcgtca gggaccgggg cccggaggct gggtcgatgg tgctcagtcc tcgaggtcct
     6361 cgtcgctgtc caggtcgatg atgtgcatcg ccgcttcctc ggccgacgcg ccgccgccgt
     6421 cgatccccac gtctccggcg aacacgtcgt tctcgaactc cgtgttgccg tcttcggcgc
     6481 tcgcaacgat gcgaccgctg cggacccgcc cggcctgtcc gacatagtcc gcgtcggcgt
     6541 agatcgagtc ggggtcgtcg ccgccgatct tctccttgcg gccccgctgc tcgcccgact
     6601 cgtcatcggg cgcgccgtag gcggaatacg ggtcgggctc ctcttgaagg atgcgctggt
     6661 cgatcgtttc gtggccacgc tgctcggccg ccgtcgtgcc gtaggcatac gacccgcggg
     6721 ggcgatcggg aggggagaaa ccctcgtcga gaacgtcgtc gacgccacgg tcgatgaggg
     6781 tgtcaccttg ctgcagctgg tcctcgtcgt cgaccgacca cacctccagc ccatcgccga
     6841 tggtgtcgtt gtcgaaggca gcctcgtcgg tgctcataca caccactctg ccacttccct
     6901 gcgcatcaag cgacgtggtg aaggcctcaa gatccccacg gagatcctcc ccgcgtcaac
     6961 gcctcaatcg gcgaggacgc gcaccgtcgc gagggtcggg tcagcggcat acgcgatgcg
     7021 cgttccggcg gcacgtgcgg cctgcagcgc ggacatcgtc tcctcggtga tcagctcgcc
     7081 cggagccagc accggtatgc cgggtggata gggcgccacc aactcaccac acgtccgccc
     7141 gacggcgcga gcggccgcca ccgtctcctg tcgggcgaag aacgccgcgc gtggcgggca
     7201 gacctgttgc ggggcaacgc tccatgcgat cgaggcggcc gtgggccggg gcgtgccgcg
     7261 gtggacctcg acgagctcaa cgagggcgtc ctcgagccga cgcaacgttg tgggggtgtc
     7321 ggcgagggtc accatcgcaa cgatggtgtc gcggtcggcc aactcgaccg gcatccgccg
     7381 cgcgatgagg tccgcctcga tctgcgtgcc gtcggcgccg gttccggaca ggacgaggca
     7441 cactttgact gggtcgacgc ctggcccgtc gagcaccacc aaccccggca ccgcgcgcag
     7501 ccgagcccgg aggtccgcga cctgcccgag gaggtcgccg agcaaggccg gcccgtcgcg
     7561 ctccaccagg gcgcgactgg cgtcgatgct ggcgaggatc gccccggagg ggctcgtcgt
     7621 ggcggtggcg tcgacggcgg cagcgaggcg ggccgggtcg atccgctcgg tccgggccag
     7681 gacgagcgcg ccctgcgacc acgcaggcag cgtcttgtgt gcgctggtca ccaatgcgtc
     7741 ggcgccgcga gccagcgcgt ggccgggcag ggccgggtgg aatccgaagt gcgcccccca
     7801 ggcggcatcg acgacgaggg gtatgccgtc cgccccgccc cgcgcccggc tgccgcggtg
     7861 ggcgtgagcc acggcggcca ggccctcgat gtcggtgagg gtcccgacat acgacggatc
     7921 cccgatgaag acggcgacgg cgtccggtgt cagggccaac gcgtcggtga gggcccgcgg
     7981 aggtatgccg cgcggcatac ccaggtcgtc cacgtcgggg cgcacccaga cgggggtgag
     8041 gccggcgagg accaacccga gcagcagcga gcggtgcaag gtgcggctga cgatgacccg
     8101 gtcacctggt gcgccgaccg cgagggcgag ggcctggttg ccgtgagtgg acccgccgac
     8161 ggagaaccga cagacgtccg cgccccagag cctggccgcc cgcgcctctg cagcggccag
     8221 cacgccgtgg gcctgagaca tcgtgtccag acccgcaaag agcgggacat cgccggcgac
     8281 gacatcgccg acgaggtcca ggcgctgctt gtgcccaggg atggtgaagg gggtcggcgc
     8341 gcccccctgg aatgccagcc aggcgtccag ggcgggcgca tcaccccgca gcccgcgagg
     8401 atcgctcggc atcgcgccgc gctcgcgtcg tcaggccgcc ggccgagcgg aggtcagtgc
     8461 gcctcggcga gcaacgactg gatccgggtc actccctcga ccaggtcgtc gtcgcccagg
     8521 gcataggaca gccggaggta gccgctgggg ccgaacgcct cacccgggac gaccgcgacc
     8581 tcgacctcgt cgaggatcag ggaggcgagc tcggcggagg tcagcgggcg agcgccacgg
     8641 atcgtctgac cgaggacacc cttgaccgac gggtagacgt agaacgcccc cgagggggtg
     8701 gggcaggtga caccgtcgat ctcgttgagc atggcgacca tggtccggcg gcgacggtcg
     8761 aaggcgacct tcatcgcgtc gaccgccgcg aggtcaccgc tgagggcggt gatggcggcc
     8821 cgctgggcga cgttgttgac gttgctcgtc aggtggctct gcaagttggt ggctgcagcg
     8881 atgacgtcct tggggccgat catccagccg acccgccacc cggtcatcgc gtaggtcttg
     8941 gcgactccgt tgaggatgat gcacgtgtca gcgagctcgg ggacctcgac gacgatgctc
     9001 gcggcgcgga cgccgtcata ggtgaggtgt tcgtagatct cgtcggtgat gacccagatg
     9061 ccgtgatcgt gtgcccaccg tccgatcgcg cggacttgct cgggggagta gaccgctccc
     9121 gtggggttgg acggcgagca gaacagcagc gccttggtgt tctcggtgcg ggcggcctcc
     9181 aactgctcga ccgtgaccag gtagccctgg tcctcaccgg cgaagacctc gacggcgcgg
     9241 ccgccggcca gcgcaatggc ctccgggtag gtcgtccagt aaggggcggg cagcagcacc
     9301 tcgtcacccg ggtcgacgat cgaggcgaac gcctggtaga cggcctgctt gccgccgttg
     9361 gtgacgagca cggcggtggc gggcacggca tacccgctgt cgcgcaacgt cttggccgcg
     9421 atcgcctcgc gcagctcggg gagcccggcc gcgggggtgt agcgatggtt ggcggggttg
     9481 cgggccgccg cgatggccgc ctcgacgatg tagtcgggcg tcgggaagtc cggctcgccg
     9541 gcaccgaagc cgatgaccgg ccggccttgg gccttgaggg ctttggcctt ggcgtcgacg
     9601 gcgagggtgg cgctctcggc gatggcaccg atgcgctggg aaacacggcg ggggacgatg
     9661 agatcgttca cgggcgtcat gctcccactc ctgggcgcct gttcgggagc ctggtccgca
     9721 ctgcgggcga atcccgtgga gtgtgcccgt ggcgtatgcc ggttcgcgcc ggggaccagc
     9781 ctcacgtatg ctttcccttc ggttggcttt ccagaccatg ctcaggcgtg cccgcagcaa
     9841 ggctttcttg acagtcgatc gcctccggat ctccccaggt ccggtggagg gcaatagctc
     9901 aattggtaga gcaccggtct ccaaaaccgg cggttggggg ttcgagtccc tcttgccctg
     9961 cgtcacgtag taagacctga tcgagaagag gccgagtgag cgagtcgagc accgcgcgga
    10021 cgggcagccg tgctgtccgc cagcggcccg gtgacggtaa cccgttcagc cggttcttcg
    10081 ctgcgctgac cctgttcatt cgtcagatcc tcgacgaact gcgcaaggtc gtccgcccga
    10141 cgggttcgga gctgctcaac tacacgatcg tcgtcatcct cttcgtggtt accatcatgg
    10201 cgatcgtcgc ggggctcgac gtcgtcctgc accgcctcgt cctgtgggtc ttcgccggca
    10261 gttgacggtg acgttcccgc tgtcgcgggc aggtctcatt cgggtgctcc ggcgcccccc
    10321 gacgcaagga aagtaggtcc aggccgtggc cgaccaggac gaagtcctcg acatcgaggc
    10381 ggacgagacg cacctcgatg acgttgaggt ggtcgaggtc gacggcgctg acgccgacct
    10441 ggttgtcgac gaggctgacg ctgacgacac ggctgacgcc gacgacacgg ctgacgccga
    10501 cgaggcagag tcgtcggaag aggccgctga ggtcatcgac ccggtcgccg agttcaaggc
    10561 caccctgcgc gccaagccgg gcgagtggtt cgttgtgcac tcctacgcgg gctatgagaa
    10621 ccgggtcaag gccaaccttg agacccgcat catcagcctc aacatggagg actacatctt
    10681 cgagatcgac gtccccatgg aggaggtgac cgagatcaag aacggccaga agaagctcgt
    10741 ccgccgcgtt cggatgcccg gttacgtcct cgtgcggatg gatctcaccg acgcctcgtg
    10801 gggcgccgtg cggcataccc ctggtgtcac cggcttcgtc ggcaacgcgc accagccgat
    10861 cccgctgacc ctcgacgagg tctaccgcat gctggccccc gacctggccc ccgcggccgg
    10921 cccggcgggc gcaggcgcgg tgggcaccgg cagcaagccc aaggaggtcc gggtcgtcga
    10981 cttcgagatc ggcgagtccg tcaccgtcat ggaaggtccg ttcgagaccc ttccggcgac
    11041 gatctccgag atcaaccccg acacccagaa gctcaaggtg ctcgtctcga ttttcggccg
    11101 ggagaccccg gtcgaggtgt ccttcggtca ggtcgccaag atctgatcga gtcccgccga
    11161 tcgtgcatca gccggtcggc actgcaaccg ggtcatcgcc cacggcgtcg gcctaaacat
    11221 caaggagaac agcaatgccc ccgaagaaga agaagatcac cggcttcatc aagctgcaga
    11281 tcaacgccgg tgctgccacc ccggccccgc ccgtcggccc ggccctgggt cagcacggcg
    11341 tcaacatcat ggagttcgtc aaggcctaca acgcggccac cgaacaccag cgcggcaacg
    11401 tcatcccggt cgagatcacg gtctacgaag accgctcgtt caccttcgtc accaagacgc
    11461 cgccggccgc cgagctgatc aagaaggccg ccgggatcgc caagggctcc agcgagccgc
    11521 acaagaccaa ggtcgccaag ctcaccgccg accaggtccg cgagatcgcc cagcagaagc
    11581 ttgaagacct caacgccaac gacgtcgaga tggcggcccg gatcatcgcc ggcaccgctc
    11641 gctcgatggg tgtccagacc gagggttact gaccctcggc cggcgccgac acggcatacc
    11701 acctgggtat gccgtgtgtg ggagagccga gcgcggctcg cgagaccaca actccagttg
    11761 cacgaccacc gttttgaagg agcagaagtg aagcgcagca aggcctaccg ggccgccgtt
    11821 gacaagatga ccccgggcaa ggtgtacggc ccgcttgagg ccgtccgtct cgcgaaggcg
    11881 acgtccgcga cgaagtacga cgcgaccgtc gaggtcgccc tccgcctcgg ggtcgacccc
    11941 cgtaaggccg accagatggt ccgtggcacg gtcaacctgc ccaacggcac cggcaagacc
    12001 gcccgggtcc tcgtcttcgc caacgccgac aaggccgagg ccgcccgcgc cgcgggtgcc
    12061 gatttcgtcg gcgccgacga cctgatcgag aagatcaccg gtggctggct ggacttcgac
    12121 gccgtggtcg cgacccccga catgatgggc aaggtcggtc gcctcggtaa ggtcctcggc
    12181 ccgcgtggcc tcatgcccaa ccccaagacg ggcaccgtga ccatggacgt cgccaaggcc
    12241 gtcaccgaca tcaagggcgg caagatcgag ttccgcgtcg acaagcacgg caacctgcac
    12301 ttcatcatcg gcaagacgtc gttcgacgag cgcgccctgg tggagaacta ctcggcagcc
    12361 atcgacgagg tcctgcgact caagccgtcg gcctccaagg gccgttacat cgagaaggcc
    12421 acgatggcca cgacgatggg ccccggcatc ccgctggact tcaccaagac ccgcaacctc
    12481 ctggccgagg acgaggcctg acctcactcg ccccgatggc gcccgcggac ggaacctcgt
    12541 cccgggcgcc ttcgtttggc cccgtggagc aagggtcggc cagcgttgtt agggtggtga
    12601 gcgatcagtt gtcgtcccac attcctcagg gggtatgccg tgaagtccgt tctcatgtgc
    12661 gccacagttc tcgtggcagc agtctcgttg gccggttgtg gcggaaccgc agcacccgcg
    12721 gtggccacgg cgaccacgac cccggccgcg agcaccagcg ccgcatcgac tgcgccgacg
    12781 acgaacgccc ccccagccgc cgatggcatc gacggcactg gcatcgccgc gaagatggtc
    12841 atggccctgg tcaaggccgg cagcggcagg tacgacatgg tgtcgtcggg cgcggccggt
    12901 ggccagcagg tgacgatgac cgccaagagc gagttcgtcg tcgtcaacgg cgccatggac
    12961 tcgaaggcga cgatgagcgt cgccggtggc tcgatggagg tcgtcagcgt cggcggcgtg
    13021 atgtacatga agggcctgat caagtccaag gacggcaagc cctgggtgaa gatcgacccc
    13081 aacgggaccg atgagttcag caagcggatg gcgccgcaac tcaagtcggc cgctgacctg
    13141 cgctcacagg tcgaggtcta cgaagggtcc aaggcgaccc tgatcggcac cgacgccgga
    13201 gtccggcact accgcctcac gggcgtgtcg agtgcgcccg cccagccgtt cgacatctac
    13261 ctcgacgcat cggacttgcc ggtcaaggtg acgacgaagt cggactcaat ggagatgacg
    13321 atcacctaca gtggcttcgg cgatgcggtc acggtgacgg cgccgccggc cgcgcaggtg
    13381 ggcccgcctc cggcgatgtg atcgagcccc cgggcacggg ggatggtcgc gagcaagggc
    13441 acccttcatt gatcgggacg atgggcagca taggcacagc cgattgatga tgacttgggt
    13501 cgagacgtta attgatatag attgactatc atcagttttc cgtaccgaac cagcgaggtg
    13561 tacccacaga tggctctcat cgggtccgag atcaagccgt tcaccgccac cgccttcaag
    13621 agcggttcgt tcgtcgtcgt cagcgagcac gacctcaagg gcaagtggtc cgtggtgttc
    13681 ttctacccgg ccgacttcac cttcgtgtgc ccgaccgagc tcggcgacat ggccgaccac
    13741 tacgccgagc tccaggccat gggtgtcgag gtcttctccg tgtcgaccga cacgcacttc
    13801 acccacaagg cctggcacga ctcgtccgac acgatcggca agatcgacta cttcatggtg
    13861 ggcgacccca ccggcgagat cacgaccaac ttcaaggtca tgcgtgaggg ccagggcctg
    13921 gccgaccgcg gcaccttcgt cgtcgacccc gacgggatca tccaggtcat ggaggtcacc
    13981 tgcgagggag tcgggcgcaa cgccgccgag ctcgtgcgca aggtcaaggc cgcccagtac
    14041 gtccgcaacc acccgggcga ggtctgcccg gccaagtggg aagagggcgc cgagaccctc
    14101 gctccgtcgc tggacctcgt cggcaagatc tgagcctcgt ctcaccccgc gcgggttcac
    14161 cctcgcgcag cctcggtatg ccgcgtgcgc acctgtgcgc gcggcatacc ggatggcttc
    14221 tcccgcacct ccgacgttcg cccgcgcccc gctaggagcc gaccatgctc gacccgaacc
    14281 tcactgccgc gctgaagtcc tacctggagc ggctcaccct gccggtcgag ctggaggcca
    14341 gcctcgacga ttcccgcgcg tcggcccaga cgcgtgagtt gctgggggag atcgcggcgt
    14401 tgtccgcact ggtcacggtg acgtccggcg acgatgcccg caagccgtcc ttcgccgtca
    14461 agcgggccgg caccgacatc caggtgcgct tcgccggcgt gccgctcggg cacgagttca
    14521 cgtcgttggt cctcgccctg ctgcaggttg gcggcgtcgc cccgaagatc tcggaggaga
    14581 ctgcggcggc gatccgggcg atcgaggggg agcaccactt cgagacctac atgtccctca
    14641 cctgccagaa ctgccccgac gtcgtgcagg cgctcaacgc gatgagcgtc ctcaacccgc
    14701 ggatcaccca cgtcaccatc gagggtgggg ccttccaggg cgagatcgag tcgcgcaagg
    14761 tgctggcggt gccgaccgtc tatcgaaacg gcgagttgtt cggtcagggc cggatgaccc
    14821 tcgaagagat cgtcggcaag ctcgacagcg gagcggcggc tcgcgacgcg cagcggctcg
    14881 aggcgatgga tcccttcgac gtcctcgtcg tcggtggtgg cccagctggc gcggccgcgg
    14941 caatctacgc atcccgcaag ggaattcgca ccggcatcgt cgcggagcgg ttcggtggac
    15001 aggtcatgga caccatggcg atcgagaact tcatctccgt cacccacacc gaaggcccca
    15061 agctggccgc ggccctcgaa gagcacgtgc gctcctacga ggtcgaggtc atggagttgc
    15121 agcgggccgc tgcgctcacc ccggccgcgg agccgggcgg gctcgcgcag gtgcgcctgg
    15181 acaacggcgc ggtgctctcg gctcgcaccg tcatcctcgc caccggtgcc cgctggcggc
    15241 acatgggcgt cccgggcgag gaggactacc gcaacaaggg cgtcaccttc tgcccgcact
    15301 gcgacgggcc gctcttcaag ggcaagcggg tcgctgtcat cggcggcggc aactcgggca
    15361 tcgaggccgc catcgacctg gccggcgtcg tcgggcacgt cacggtcctg gagttcatgg
    15421 acgagttgcg ggccgacgcc gtcctgcagc gcaagctcta cagcctgccc aacgtcgagg
    15481 tcatcctcag cgccaagacg accgaggtgc tcggcaacgg tgagcaggtc accggcttgg
    15541 actacgagga ccgcacgacc ggggcgtcga ggcacctgga tctcgagggg atcttcgtcc
    15601 agatcggctt gctgcccaac accgagtggc tcgccgatgc gctcaccctc accccgcgca
    15661 aggagatcgt catcgacgac cgcggtcaga cgtcagccac cgggatcttc gccgccggcg
    15721 actgcaccac gaccccctac aagcagatcg tcatcagcct cggcgccggg gcgaccgctg
    15781 ccctcggggc cttcgaccac ctcatccggt caacggcgcc ggaggccagc ctcgtcgacg
    15841 cctgatggcc ggaggcgatc tggccggcgg gcggcatact gaggcggtgc agaggcggcc
    15901 atgaaccttc gtgacctgca atatctcgtc gccgtggccg accaccggca cttcggacgg
    15961 gcggcgacgg cgtgtgcggt gagccagccg acgctctcga cccagttgaa gaagctcgaa
    16021 gtcgagttgg gggttcagtt ggttgagcgg ggcggccggg ccgtcatcct cacccccgtc
    16081 ggtgaccagg tcgtcgcccg ggcccgagcg gtgctggccg aaggcgaagc gatccgccgg
    16141 atcgcccgtc aggctcagga cccgcgggcc gggcggctgg ccctcggggc gttccccacg
    16201 atcgcgccct acctcttccc gcacgtcctc ggtgagctgc gcaggcaact gcccgacgtc
    16261 gagttgctgc tcgtcgagga caagaccgct gccctgctca cccaactgcg cgaggggtcg
    16321 ctcgacgcgg tcgtcgtcgc cctgccggtg gacgaggagg gcttgcacgc cgaaccgctc
    16381 ttccgggagg ccctgctgtt ggccgcgccg gcgacgcacc ccctcggccg ggacacctcg
    16441 ccggtcgggt tggaggacct cgcgggggag agcgtgctgc tgctcagtcg cggccactgc
    16501 ctgcgcgagc aggcccttgc cgtatgccgc gcggccggcg ccgtcgagcg ccccggcttc
    16561 cgggcgacca gcctggacac cttgcggcac atggtcgagg cgggcctcgg cctgacgatc
    16621 ctgccccgcc tgtcgctccc ggaagcccgg gagttgccgc ccgaggtggt cgtgcgcgag
    16681 ttccgcgagc cgacgcccta tcgcgatctg gccctggtct ggcggctggg cagcgtccgg
    16741 gacgccctgt tgccggaggt ggctcaactg ttgcgccggc tgccgaccgg gctggtgacg
    16801 ccgctctagc ggtagccgag ctgcgcgtcc gggaagctgg acagccgcga gatcttgacc
    16861 ggttcaagat aacgggggat agtggcgcta tgcccgctca cacggaggtg gcccgcgcgt
    16921 tgcgcgatgc cgggctgcgg gtcacccaac cgcgactggc cgtgttggcg gctgttcacg
    16981 accatccgca ctccgacacc gagggcttga tcgttgccgc tcgggccgag ctggcggggg
    17041 tgtcccatca ggcggtctac gacgtcctgc gcgcgctgac ggacgccggg ctggtgcgcc
    17101 ggatccaacc cccggggtcc atcgcccgct acgaagcccg cgtgggcgac aaccaccacc
    17161 atctcgtctg ccggcactgc gcctcgattg tcgatgtcga ctgtgcggtg ggcgagcgcc
    17221 cctgcctcac cgcgtccgac gaccacggct acgtcatcga cgaagccgag gtcgtctatt
    17281 gggggacctg cccggcgtgc ctcgccggga gccccccgcc cagttcctcc gacacccgct
    17341 ccacccacca ccccgaagga agtgagccat gaccgatcac gacgctgctg ttggcgaccc
    17401 gacgtccgac ctgagcgaag ccagctgccc cgtggcgcac gcccgtcccc agcaccccac
    17461 cgaggggtcc ggcaaccgga cctggtggcc caaccaactc aaccttcgaa tcctcgcgac
    17521 caacccgccc gccgggaacc ccatggccgc aggcttcgac tacgcagcgg cgttcggcgc
    17581 gctcgacctg ccgagcgtca agcgcgacat cgagtcggtg ctgaccacct cgcagccgtg
    17641 gtggcccgcc gactacggcc actacggacc gttgatgatc cggatggcct ggcacagcgc
    17701 cgggacctat cgggtcagcg atggtcgagg gggcgccggg gccggacagc agcggttcgc
    17761 gccgctcaac agttggccgg acaacgccaa cctcgacaag gcccggcgcg tgctgtggcc
    17821 ggtcaagaag aagtacgggc gcgccctgtc gtgggccgac ctgatgatcc tcgccggcaa
    17881 cgtcgccctt gagtcgatgg ggttcaccac cgccgggttc ggcgggggcc gggccgacgt
    17941 ctggcaggct gacgaggagg tctactgggg gcccgaggac acctggctcg gagacgagcg
    18001 ctacaccggc gaccgcgacc tggagaaccc gctggccgcc gtgcagatgg gtctgatcta
    18061 cgtcaacccc gaaggcccca acggcaatgc cgacccgctg gcctccgccc gcgacatccg
    18121 tgagacgttc gcccggatgg cgatgaacga cgaggagacc gtcgcgctca ctgccggtgg
    18181 gcacacgttc ggcaaggcac acggcgccga cgacccgaac cgctacctcg ggccggagcc
    18241 ggagggtgcc gacctcgccg cgcagggtct gggctggctc aacagcaaga acgccggcaa
    18301 cggcgtcgac acgatcacca gcggcctgga aggggcctgg acgcccactc cgacgacctg
    18361 ggacaacggg ttcttcgaga cgttgttccg gtatgactgg gagttgaccc ggagcccggc
    18421 aggcgcccag cagtggcggc ccaagggtgg ggcgggcgcg tcggacgttc ccgacgccca
    18481 cgacccgacc gtccggcatg caccgatgat gaccaccgcc gacatggcga tgcgcgtcga
    18541 cccggtctac gagcagatct cccggcgctt tcttgagcac ccggacgagt tcgccgacgc
    18601 cttcgctcgg gcgtggttca aactgaccca ccgcgacatg gggccgatcg agcgctacgt
    18661 cggttccgag gtgcccgccg agcggttcat ctggcaggac ccggtgccgg ccgtcaccca
    18721 cccgttgatc tctgacgccg acgcggcaga cctcaagcga acgatcctcg cgtccggcct
    18781 gtccgtcccc gccttggtcg ggacggcgtg ggcgtcggcg gccaccttcc gcggcggtga
    18841 caagcgcggc ggcaccaacg gggcgcgaat ccggctggaa cctcaaaacg gttgggacat
    18901 caacgaaccc gagcggctgg cctccgttct gaccaccctc ggtggtatcc aggagacctt
    18961 caacgctgac caccccgcgg gtccgcacgt gtcgatggcc gacctcatcg tcctcggtgg
    19021 caatgccgcg gtcgagcagg ccgcccgcaa tgccggcgtc gaggtcaccg tgcccttccg
    19081 tgcgggtcgc ggcgacgcgg tccaggagat gaccgacgtc gagtccttcg aggtcctcaa
    19141 gcccgccgcc gacggcttcc gcaactacgc cggcaagggc ctgcggctcc ccgccgaata
    19201 ccacctcatc gaccgggcta acctgctcac gctcagtgct cccgagatga cggtgctcgt
    19261 cggcgggctg cgggtgctgg gtgccaacta ccaggggtcc cgtctcggtg tcctcaccga
    19321 tcgcaccgac accctctcga acgacttctt cactaacctg ctcgacctcg gcatccactg
    19381 gacgccgacc gacgaggccc aggacacgtt cgagggccgg gaccgggcca ccggcgaggt
    19441 ccgatggacc ggcagtcggg tcgacctggt cttcggttcc aactccgagt tgcgagctct
    19501 tgccgaggtc tatgcgagcg acgacgccca ggcgagcttc gtccacgact tcgtcgctgc
    19561 gtgggacaag gtgatgaacc tcgggcggta cggccgcgac tgagcctgtt gcccgacgga
    19621 cgggcccggt ggtggttacg ccaccgggcc cgtccgtgag cctgtccgtc cgtctggagg
    19681 ccggtcgacc ggacgccgtt cggtagcgga tttggctgcc ggcctgcgcc cgactaccct
    19741 gagcaacgac caatgaccgc cggttgtcgt gccgtccgcc aggaaactgg aggccgggat
    19801 gaccgaaggt cccgccacga ggcgggcgac cagcgcaggc cacgcaagcg cccggcatac
    19861 ctcttggtat gccgcgaagc cccgtgcgcc tcgcgccggg gcttttctcg tgggggactt
    19921 cgcagcccga accggcgtcg caccgctagg taaaggaggc ccgatggcca cc
//
LOCUS       DAORPW010000032        19156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_497_length_19156_cov_699.956756, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000032 DAORPW010000000
VERSION     DAORPW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 19156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19156
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_497_length_19156_cov_699.956756"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1102)
                     /locus_tag="PLB86_04490"
     CDS             complement(47..1102)
                     /locus_tag="PLB86_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HRC64425.1"
                     /translation="MTLWRADLLSDTVTRPTPAMRQAMARAEVGDDVFGEDPTVQALQ
                     ERVAGLLGHEAALFTPSGSMANQLGLRLHVRPGEELLCDSLAHVVRAELGAAAAFSGI
                     TSRTWTAPRGLLDPPDPIALLQTGVGPYQVCTSLVVVENTHNFGGGTVQPLWAIRQVR
                     AATRPRGVAMHLDGARLWNAHVASGVALAEYAAHFDTVSVCLSKGLGAPVGSLLVGSA
                     DAIAEARIWRKRFGGGMRQVGILAAAGLYALDHHVERLADDHARAQRAARAFADAAPG
                     CVDPETVETNIVVLTTTDTGWSPAELVAAVAAKGVRGYAAGPEALRLAWHLDVDDAAT
                     DYAIDVVTTLLRARPTP"
     gene            complement(1146..2486)
                     /locus_tag="PLB86_04495"
     CDS             complement(1146..2486)
                     /locus_tag="PLB86_04495"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HRC64426.1"
                     /translation="MHGQLAAAAAERTAGQQPTWPDQGALAVAVAELAQYPPLVFAGE
                     CDVMRERMAATARGEAFVLMGGDCAETFAGATANNIRDRIKTVLQMAAVLTYGASVPV
                     VKVGRMAGQYAKPRSADTETRAGQTMPAYRGDMVNDFAFDPVARIPDPQRLVTAYHRA
                     SATLNLVRAFTTGGFADLRRVHDWNTGFVENAANARYERLAHDIGKAMKFMSACGADF
                     DAMRTTEFFAAHEALLLDYERPLTRIDSRTGQAYDTSAHFIWLGERTRELEGAHVDFL
                     ARVRNPIGVKLSAKADPDDVLRLIDRLDPQREPGRLSLITRMGASRVTEALPALIERV
                     HGSGATVTWICDPMHGNTFESQGGYKTRNFDDIVEEVRGFFAVHKELGTVPGGLHVEL
                     TGNDVTECIGGSTKILDADLEKRYETVCDPRLNHRQSLELAFLAAEMLSGDVPS"
     assembly_gap    2537..2636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2637..3383
                     /locus_tag="PLB86_04500"
     CDS             <2637..3383
                     /locus_tag="PLB86_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HRC64427.1"
                     /translation="VPAAVPGIGAVATQSFAKVSYKADSLALLRAGASASDVVARITD
                     ADPLRETRQVGVVGDGGAATFTGADCSDWAGGVSGGNDTDGWYAVQGNILVGPQVVHA
                     MEQAWLATADLPLSRRLLAALLAGDAAGGDRRGRQSAALYVVDESAGYDGSGIVVDLR
                     VDEHDDAASELARLVELNDLYFGSPEDVQPLQGPLADEVRERLGRLGYRGELDAALGE
                     WAGVENYEMRLTPDGIDARVLSQLRAATGG"
     gene            3427..3924
                     /gene="tpx"
                     /locus_tag="PLB86_04505"
     CDS             3427..3924
                     /gene="tpx"
                     /locus_tag="PLB86_04505"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRC64428.1"
                     /translation="MATTALRGTPVSTSGELPDVGDHAPEFTLTSTSLADVSSEGLVG
                     SRVVLNIFPSVDTGVCAASVRRFNELAGSLDNTTVVNVSLDLPFALSRFCGAEGIENV
                     TSASAFRSTFGDDYGVRMVDGGLRGLFARSIVVLDTDGTVLHTQVVPEIAQEPDYDAA
                     INALS"
     gene            complement(4007..4759)
                     /locus_tag="PLB86_04510"
     CDS             complement(4007..4759)
                     /locus_tag="PLB86_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC64429.1"
                     /translation="MKGQANNPRQPGPDGVDPLAGADGDLGSGLADRPLRVVVADDHP
                     MWRDAVARDLVDAGFSVVGTAADGPSAVSRTLATAPDVLVLDLNMPGMRGSAVCAELA
                     AAGSPARILVLSVSGEPEDVLSTIKAGATGYLVKSASREEFLEAVRRTAEGRAVFTAG
                     LAGLVLGEFRRLGQQPPPGEGDVPAAPELTERETEVLRLVATGLSYKQIAAQLFISHR
                     TVQNHVQNTLAKLQMHNRVELVRYAIERGLHE"
     gene            complement(4759..5910)
                     /locus_tag="PLB86_04515"
     CDS             complement(4759..5910)
                     /locus_tag="PLB86_04515"
                     /inference="COORDINATES: protein motif:HMM:NF039922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5931 domain-containing protein"
                     /protein_id="HRC64430.1"
                     /translation="MTPQSTPATDDDSLVLSALWGALRVFRVLALVYALWSIWVRHAE
                     MARLDLALMVIGVLSVWTAYMYVSRRRDLRIHVMEMVLASAAVMSTRWFDTPLSATTG
                     DTTIPGVWQNVPVVAIALILGWRGGLIASLVITAVMIAQVGQFDSEPLSNAGLIVMLG
                     TCLGFGADVARTEQAELRRALARQAEVAERDRLARVVHDGVLQALAFIHRRGRDIGGE
                     SARLGMIAAEQELRLRALVIGMPLPELEVAIAGPVDLRRALDAAVTESATLVASAEPV
                     ELDSAKAQEVVAAVEAALDNVRKHAGPQAQAWVVVDDLGADVVVTVRDDGPGVSQERI
                     AEAAQSGRLGVSSSIKGRIEDLGGRARYLTSPGGGTMVEMWIPKGRAAT"
     gene            complement(5936..6877)
                     /locus_tag="PLB86_04520"
     CDS             complement(5936..6877)
                     /locus_tag="PLB86_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC64431.1"
                     /translation="MFYWFLKTVVLGPILKLLFRPWVEGAEHIPERGAAILASNHLSF
                     SDSIFLPIVMSRRITFPAKMEYFTGSGVKGRLTAWFFKGVGQIPIDRSGGRASEAAIS
                     SGLKVLRRDELFGIYPEGTRSPDGRLYKGKTGIARMALEAGCPVIPVAMIGTDKAQPT
                     GQKLPKIMRIGVRIGAPLDFSRYEGMENDRFVLRSITDEIMYELMLLSGQEYVDVYAT
                     SLKKNVLKDTVQTAVAKAQEIQKTAQEIQKTAAEAAKAATEAARAVTESAKTRATHPQ
                     EPLETHEAVEAPDSATSTGSVDDDGPSSDEQGRLRAS"
     gene            7003..>7433
                     /locus_tag="PLB86_04525"
     CDS             7003..>7433
                     /locus_tag="PLB86_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64432.1"
                     /translation="MNDEDALEADDQGRSEPAGGPEETSATALPPLNEIDAAFASIVA
                     HWDDPTPEPLADRAADDAAGDAADLTAPAPLAPAPLPPAYPATAHVAVPVGDAPDATT
                     DHFSAAEDADEERLFGWRGHTPPPEDEHFQPPPPNLPPVHDA"
     assembly_gap    7434..7533
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7534..8108)
                     /locus_tag="PLB86_04530"
     CDS             complement(<7534..8108)
                     /locus_tag="PLB86_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRC64433.1"
                     /translation="MSAAYTIGIDIGGTKVAGGVVDVDGVILAKARADTPHRSTSPEV
                     VESTIVGVVEDLIAQVGPGQVSAVGIGAAGFVGADRATVVFAPHLSWRQEPLRDALFD
                     RIGLPITVDNDANASSWAEWRFGAGRGESHLVMVNLGTGIGGGIVLGGRVERGRFGIA
                     GEFGHMQVVPDGLRCECGNRGCWEQYSSGNAL"
     gene            complement(8105..8485)
                     /locus_tag="PLB86_04535"
     CDS             complement(8105..8485)
                     /locus_tag="PLB86_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64434.1"
                     /translation="MSVEGSDRVGSVAEEAARLLGVFAAGMPEAHLLAAGSRAAGETP
                     TAHACPECGHDPHAASPGDSVCRICPICRVLNVVKAISPETLDKIADVVDLVSEGLRG
                     FAANRRTETPPPAPTGGEQPQEPV"
     gene            complement(8488..8931)
                     /locus_tag="PLB86_04540"
     CDS             complement(8488..8931)
                     /locus_tag="PLB86_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC64435.1"
                     /translation="MAERTESSIHVDAKPGVVLDVIADFEAYPEWAAEVKRVSVLSEE
                     GDGWADRVEYTLDAGVLKDTYTLDYEWDISDDGTGVVSWSLVRATVLKAMNGSYTIAR
                     DKAGSKVTYRLAVDLNIPMLGMLKRKAEQQIVDAALKELKKRVEG"
     gene            complement(8995..10470)
                     /locus_tag="PLB86_04545"
     CDS             complement(8995..10470)
                     /locus_tag="PLB86_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64436.1"
                     /translation="MTAFGRRSAWGRWLPAADVDGRRASLVTAIVTAHGAAVVTALVA
                     GVVVALVMSGCTPNAATPSTTATASTRANSGANAGADAVAGAGSEASEAARTALTTLM
                     AARSRALETGDRTAWLATIADPHGPDGSAEEAAYAGLVALGVREVIVSEVRRSATQAT
                     SAAPAAEESQHAQTLTTQTWTGSMRLGYAIPGFDRGVRWVARTVTLTQVAGQWLIQRW
                     VGPADRWEVFDLSPLQVARSERALVAGPVAQDVLRDRLAEVEVGQDRVAAALGRAFPA
                     VVVVPATTDQAALLLGNGAPSSEQSPLAGQVAATTHGPREPSATAVADRVVLDPQGMG
                     RLTPAGRRVVLTHELTHVSVRAGTLHDLPLWLSEGFAEWVAFRDEGMDVTVVAARLLS
                     QVRASGPPATLPTATDFAGTAGDPAAAYQESWLAASRIATLAGPEGLVGLVRRVGGVP
                     GASSSPAGPVDPAAALTEVLGQTMSQFVAGWQSELVTLAAG"
     gene            complement(10521..10757)
                     /locus_tag="PLB86_04550"
     CDS             complement(10521..10757)
                     /locus_tag="PLB86_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64437.1"
                     /translation="MIVTCATCPVRGLRCDDCVVTALATISVGPPGERPLDAKERRAV
                     GLFVSAGLLDSGYAATLTSTVDSGRVGRVGRAVG"
     gene            10900..12597
                     /locus_tag="PLB86_04555"
     CDS             10900..12597
                     /locus_tag="PLB86_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HRC64438.1"
                     /translation="MHAVQGTLDDLGTPLHAVTFVVVDLETTGGSANECAITEVGAVK
                     VRGGEVLGEFQTLVNPGSPIPAFIQVLTGISDGMVAQAPRIDTVLPAFLEFARGSVLV
                     AHNAGFDITFLKAAAEATGQSWPGFKVVDTVTLARQLVSKDEAPNRRLGSLAALFGAD
                     TTPDHRALHDARATVDVLHALLARVGNVGVQTLEELASYSSRVTTAQRRKRFLADDLP
                     TAPGVYVFKDAKGRPLYVGTSRNIRVRARSYFTASEQRTRMAEMVSVAASIQPIVCQT
                     TLEAQVRELRLIAEHKPRYNRRSTRPERALWVKLTAEPYPRLSIVREVRDDLASGARY
                     AGPFGSRAAAEAAVAAVHDVLPLRQCTTRLSTKGTGSACVLAEMGRCGAPCEGRQSLS
                     EYAVHVETAAALLVGDGRSMVDALCERMTVLAKAERFEDAGALRDRLGHLVRGLARAQ
                     RLAPLAASPELVAARRTDAGAWELVCVRHGRLAGTSLAPRGADPLVYVESLRATAEAV
                     PPPAGPAPAAYPEETELVLRWLESPGVRIVSLDGEWTCPVGGAGAAKATFDDLRRGA"
     gene            12632..12910
                     /locus_tag="PLB86_04560"
     CDS             12632..12910
                     /locus_tag="PLB86_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRC64439.1"
                     /translation="MITAIVLVTCDAARIPEVAQDIAALDGVTEVYSVTGDADLVAMV
                     RVREHEQLADVIADRLSKVEGVAATSTHIAFRTYSRHDLEAAFSLGLD"
     assembly_gap    12944..13043
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13061..14071)
                     /gene="trpD"
                     /locus_tag="PLB86_04565"
     CDS             complement(13061..14071)
                     /gene="trpD"
                     /locus_tag="PLB86_04565"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRC64440.1"
                     /translation="MLQRGHLSRQDCAWAMGEIMSGGATPSQIAGFLMALHAKGETVE
                     EMRGLADEMLAHATRIQAPELSLDVVGTGGDRAQTVNISTMAAMVCAAAGVSVVKHGN
                     RAASSRSGSADVLEALGIDLTLPAARVEALAHEVGITFCFAQQFHPSMRHVAGTRREL
                     GVPTAFNFLGPLTNPGQPTYAAVGVADARMAPIIAGVFAGRGRDAAVFRGDDGLDELT
                     LATTSTVWWVRSGDVVEHQLDPIRLGLTLSPTEALRGGDAAYNAAVVRDVFGGARGPV
                     RDAVVLNAGAALAVAAANDSGRTPAAVHADVRAGMDRAEAALDSGAADALVQRWVEAT
                     RA"
     gene            complement(14133..14585)
                     /locus_tag="PLB86_04570"
     CDS             complement(14133..14585)
                     /locus_tag="PLB86_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64441.1"
                     /translation="MTASQAHSASPAVPASVHQISVLLYSDDVTVRDAVRLAVGRRPA
                     KNVEVTSWLECATHAAVVEAVDNRRFDVLILDGEATPMGGMGLCRQLKNEIFQCPPTL
                     VLTGRVQDGWLAAWSQADAVVPHPLDPVALARAVTQLAGAGAGATAAH"
     gene            14770..15426
                     /locus_tag="PLB86_04575"
     CDS             14770..15426
                     /locus_tag="PLB86_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HRC64442.1"
                     /translation="MARVATASAIPAHPVSSGHGPATRPNMVQVGTMVWLSSELMFFA
                     GLFAIYFQLRSVSAALWAEQTAKLNVTFATINTAILVISSVWCQIGVMKAERGQASRT
                     GKLTEIGKWGMREWYVVTYLFGATFIAGQVYEYAKLVSENVTLSSDRYGSVFYMATGF
                     HGLHVTGGLIAFLLIIGRTFTTRRYTHTQAVGAVVTSYYWHFVDVVWIALFAVIYLLK
                     "
     gene            15473..16258
                     /locus_tag="PLB86_04580"
     CDS             15473..16258
                     /locus_tag="PLB86_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRC64443.1"
                     /translation="MNALAARRRHPAAIAILLFLGLALVGTAYSLLAPKPAQAAVATA
                     ADAELGKQLFLANCATCHGVNAEGTKSGPTLMGVGAASVDFQVGTGRMPLAGPNVQAP
                     RIDEVKFTEEQIAAMAAYVASLAPGPAIPDAKLTSGEGGDVALGGEIYRVNCAMCHNF
                     AGSGGALTRGKYAPSLYGVDGKYVYEAMITGPQSMPVFNDTNLNPEAKASVIAYLKAT
                     QAEPNVGGMTLGNLGPVSEGMFVWIFGLLLMIACAFWLGQKAA"
     gene            16296..17438
                     /locus_tag="PLB86_04585"
     CDS             16296..17438
                     /locus_tag="PLB86_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRC64444.1"
                     /translation="MSDQVTPTSGSASGAGTVSLDEGHHVGTGGLPATFENPGLPPHV
                     HRAADDDPKAAKRAERQVAVLFTLSILGTLGFIVSYFAIDRMTSVFLPFIGLTNLLNV
                     MLGVCLGIALLGIGFGAVHWAKTLMSDEEVVEERHAIASSDAARADFVGALAAGAESA
                     QLARRPLIKATAGAALGLFALPLGVQLAGSMGNPTKALEELKQTIWDVPKDGKPIRLV
                     RDPELTPIKASDVTIGSVFHVMPETLKELREHNVKEGHSEWHGVLEAKAKAAVLLMRL
                     NPSEFKDDVLGRKARDWGFQGIVAYSKICTHVGCPVGLYEQQTHHLLCPCHQSTFDVT
                     RDCEVIFGPAKRPLPQLKITVDDEGYLVAPQGFAEPVGPSYWERLK"
     gene            17435..19156
                     /locus_tag="PLB86_04590"
     CDS             17435..19156
                     /locus_tag="PLB86_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HRC64445.1"
                     /translation="MSTTASARTAADLRAADAKGRTGSESKAGAAGVWVDERTGLAKG
                     AAYMMKKVFPDHWSFMLGEVAMYSMIICLLTGVFLSFWFVPSAGQIPYNGSYLPLQGV
                     IMSEAYNSTLAISFDIRGGLLIRQIHHWAALMFCVAITVHMLRVFLTGAFRKPRELNW
                     VIGSILSMLAIVEGFAGYSLPDDLLSGTGIRAAEGFIRSIPLVGSYISYLLFGGTFPG
                     EAIIPRLYSVHVLLLPAVLIGLFTAHIVLVMVHKHTQFPGPGRTNENVVGFPLMPVYT
                     AKAGGFFFMVFGVLAIISALVTINPIWAYGPYDPSPVTAGSQPDWYMGFADGALRLLP
                     GFLEFELFGFTWSMNVFLGAVALIPLMYTAAGVYPWIEAWVTGDKREHHLLDRPRNAP
                     TRTAIGMAAFSFYVVLMFAAGNDIMAIRLQLSINDVTILFRMLVFVVPVIVFWVTRRI
                     CWSLQRHDREKVLHGRETGTIIRTAEGKFFERHEDIDAFERWPLVAFESPAPLALTDT
                     ADANGVSSKDAKKARRRVVWSNFYFKTRVEPVTPTELAAAHHHGEHEAISGGSAPAAI
                     KHDKHPH"
BASE COUNT         2869 a         6749 c         6306 g         2932 t
ORIGIN      
        1 attcgggccg ggggccaggc tgcggcccat tgctggtgac gctcgatcag ggcgtcggcc
       61 gagccctcag cagagtggtg acgacatcga tcgcgtagtc cgtggccgca tcgtcgacgt
      121 cgaggtgcca cgcgaggcgc aacgcctctg gccccgcggc atacccgcgg acacccttcg
      181 cggcaacagc ggcgacgagc tctgccggcg accatccggt gtcggtcgtg gtgagcacga
      241 cgatgttggt ctcgaccgtc tcggggtcga cacaccccgg cgcggcatcg gcgaaggcgc
      301 gtgccgctcg ctgggcgcgg gcgtgatcgt cggccaaccg ctcgacgtgg tggtcgagcg
      361 cataaaggcc ggccgcggcc aagatgccga cctggcgcat cccgccgcca aagcgcttgc
      421 gccagatgcg ggcctcggcg atcgcgtcgg ccgagccgac gaggagtgac cccaccggcg
      481 cccccaaccc cttggagagg cagaccgaga cggtgtcgaa gtgcgcggca tactccgcga
      541 gcgcgacacc cgacgcgacg tgcgcgttcc acagtcgcgc cccgtccagg tgcatggcga
      601 cgccgcgcgg gcgggtggcc gctcggacct gacggatcgc ccacagcggt tggacggtgc
      661 ctccgccgaa gttgtgggtg ttctcgacga caacgagcga cgtgcacacc tggtagggcc
      721 cgacgcccgt ctgcagcaag gcgatcgggt cgggcgggtc gagcagaccc cgaggtgccg
      781 tccaggtgcg cgaggtgatg cccgagaaag ccgccgcggc gccgagctcg gcccggacaa
      841 catgggcgag gctgtcgcag agcagttcct caccaggccg cacgtgtagt cgcagcccca
      901 actggttggc catcgagccg gacggtgtga acagcgcggc ttcgtggccc agcaacccgg
      961 cgacgcgctc ctgcagggcc tggacggtcg ggtcttcgcc gaagacgtcg tcaccgacct
     1021 ccgcgcgagc catcgcctgg cgcatcgccg gcgtgggccg agtgactgtg tcggacaaca
     1081 ggtcagcgcg ccacagggtc acaggactga tctccttcag atcatcgacg ggtatgccgc
     1141 gcggctcagg acggtacgtc cccagacagc atctcggcgg cgaggaaggc caactccagg
     1201 ctctggcggt ggttgaggcg tgggtcgcag accgtctcgt aacgcttctc caggtcggcg
     1261 tcaaggatct tcgtggagcc gccgatgcac tcggtgacgt cgttgccggt gagctcgacg
     1321 tgcaagccgc ccggcaccgt gcccaactcc ttgtggaccg cgaagaagcc gcgcacctct
     1381 tcgacgatgt cgtcgaagtt acgggtcttg tagccgccct gggactcgaa ggtgttgccg
     1441 tgcatcgggt cgcagatcca ggtgacggtc gctccggacc cgtggacccg ctcgatgagg
     1501 gcgggcaggg cctcggtgac gcggctggcg cccatccggg tgatgaggct cagccggccc
     1561 ggctcgcgct gcgggtcgag ccggtcgatg agccgcagca cgtcgtccgg gtcggccttg
     1621 gccgacagct tgacgccgat ggggttgcgg acccgggcga ggaagtcgac gtgggcaccc
     1681 tcgagctccc gggtgcgctc gccgagccag atgaagtggg ccgaggtgtc gtaggcctgc
     1741 cccgtgcgcg agtcgatccg cgtcaggggg cgctcgtagt ccagcagcag cgcttcgtgg
     1801 gcggcgaaga actcggtcgt gcgcatcgcg tcgaagtcgg cgccacaggc ggacatgaac
     1861 ttcatcgcct tgccgatgtc gtgggccagc cgctcgtaac gggcgttggc ggcattctcg
     1921 acgaagcccg tgttccagtc atggacgcgc ctcagatcgg cgaaaccgcc tgtggtgaaa
     1981 gcccggacga ggttgagggt cgctgaggcg cggtggtatg ccgtgaccaa ccgttgcggg
     2041 tcggggatcc gggccaccgg gtcgaacgcg aagtcgttga ccatgtcgcc gcggtaggcc
     2101 ggcatggtct gcccggcccg ggtctcggtg tcggcgctgc gcggcttggc gtactgaccg
     2161 gccatccggc cgaccttgac caccggcacc gaggcgccgt aggtgagcac ggcggccatc
     2221 tgcagcaccg tcttgatccg gtcgcggatg ttgttggccg tcgcccccgc gaacgtctca
     2281 gcacagtcgc cgcccatgag gacgaacgcc tcgccgcggg cggtcgccgc catccgctcg
     2341 cgcatgacgt cgcactcccc cgcaaacacc aggggcgggt attgcgcgag ttcggcgacc
     2401 gcgaccgcca aagccccctg gtcaggccag gtgggttgtt gtccggcggt gcgttctgcc
     2461 gcggctgccg cgagctgccc gtgcaagtcg tcgttggggt tcgtgctcac tgggacagcg
     2521 tagttgcgtc ccagtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttc
     2641 ccgccgcggt ccccggaatc ggcgccgtgg cgacccagtc cttcgcgaag gtgtcctaca
     2701 aggccgattc gctggcgctc ttgcgcgctg gagcgagcgc gagcgacgtc gtcgcccgga
     2761 tcaccgacgc cgaccccctg cgcgagaccc gtcaggtcgg cgtcgtcggg gacggcggcg
     2821 cagcgacgtt caccggcgcc gactgctcgg actgggccgg cggagtgagc ggcgggaacg
     2881 acaccgacgg gtggtatgcc gtgcaaggca acatcctcgt cggcccgcag gtcgtccacg
     2941 ccatggagca ggcctggctc gcgacagccg acctgccgct gtcccgtcgc ctcctggccg
     3001 ccctcctcgc aggtgacgcg gccggtggcg atcgacgtgg gcgccagagc gccgcgctct
     3061 atgtggtgga cgaatcggcg ggatacgacg gcagtgggat cgtggtggac ctgcgggtcg
     3121 acgagcacga cgacgcggct tcggagttgg ccaggctcgt cgagctcaac gacctgtatt
     3181 tcgggtcccc cgaggatgtc caacccctgc agggaccgtt ggcggacgag gtgcgcgagc
     3241 ggctggggag gctggggtat cgcggcgaat tggatgccgc cctcggggaa tgggccgggg
     3301 tcgagaacta tgagatgcgc ctgacccccg acggcatcga cgcgcgcgtg ctgtcgcagt
     3361 tgcgggcagc gaccgggggc tgaggaccag gcgcgccgaa cggcataccc gtgataggaa
     3421 cgtgtcatgg caaccacggc cctgcgcggc acccccgtct ccacttccgg cgaactgccc
     3481 gatgtcggcg accacgctcc cgagttcacg ctgaccagca cctcgctggc cgacgtctcc
     3541 tccgagggcc tggtgggctc gcgggtggtt ctcaacatct tcccgagcgt cgacacgggg
     3601 gtgtgcgccg cgagcgtgcg ccgcttcaac gagctcgctg gatcactgga caacacgacg
     3661 gtggtcaacg tgtcactcga cctaccgttc gctctgagcc ggttctgcgg cgctgagggc
     3721 atcgagaacg tcacctccgc atcggccttc cgcagcacgt tcggcgacga ctacggcgtg
     3781 cggatggtcg acggcggcct gcgtgggctg ttcgcccgtt cgatcgtcgt tctcgacacc
     3841 gacgggacgg tcctgcacac gcaggtcgtt cccgagatcg cccaagagcc cgactacgac
     3901 gcagcgatca acgcgctgtc ctgatctggt gagcggtcgg gccgcatcgg tccgaccgcc
     3961 tgctcgaccg accgcccgcg tgtgtcggcg tgcgcgtcgg cagcgatcac tcgtgcaggc
     4021 cgcgctcgat cgcgtagcgg accagttcga cccggttgtg catctgcagc ttggccaggg
     4081 tgttctggac gtggttctgc accgtgcgat gcgagatgaa caactgagct gcgatctgct
     4141 tgtaggacaa cccggtcgcc accaggcgca gcacctcggt ctcgcgttcg gtcaactcgg
     4201 gagccgctgg cacgtcgccc tcgccgggcg gaggttgctg tccgagacga cggaactcgc
     4261 cgagcaccag cccggccagc cccgccgtga agacggcgcg accttcggcg gtgcggcgca
     4321 cggcttcgag gaactcctcc cggctggctg atttgacgag ataaccggtc gcgcccgcct
     4381 tgatcgtcga gagcacgtcc tcgggctcgc cgctcaccga cagcacgagg atccgggcgg
     4441 gcgacccagc ggcggctaac tcggcgcaga cggccgaccc tcgcatgccg ggcatgttga
     4501 ggtccaggac cagcacatct ggtgccgtcg ccagggtccg agagacggct gacggtccgt
     4561 cggccgccgt gcccaccacg ctgaagccgg catcgacgag gtcacgcgcc accgcatcac
     4621 gccacatcgg gtgatcgtcg gcgacgacca cgcgcaacgg ccgatcggcg aggccgctgc
     4681 ccagatcgcc gtcggcgccg gcgagcgggt cgaccccgtc cgggcccggc tgcctcgggt
     4741 tgttcgcttg tcccttcatc aggtggccgc cctccccttg ggaatccaca tctcgaccat
     4801 ggtgccgccg cccgggctgg tcaggtaacg ggctcgtccg ccgaggtcct cgatccgccc
     4861 cttgattgag gacgacacgc ccagtcgccc gctctgggcc gcctcggcga tccgttcctg
     4921 ggagaccccc gggccgtcgt cgcgcaccgt gacgacgacg tcagctccga gatcgtcgac
     4981 gaccacccac gcctgtgctt gggggccggc atgtttgcgg acgttgtcca aggcggcctc
     5041 gacggcggcg acgacttcct gggccttggc ggagtccagc tcgaccggct cggcgctggc
     5101 gacgagcgtg gcggactcgg tgaccgcggc atccagggcg cggcgcaggt cgaccggccc
     5161 cgcgatggcg acctccaact cgggcaacgg catcccgatg accagcgccc gcagccgaag
     5221 ttcctgctcg gcagcgatca tccccaagcg ggcgctctcg ccaccgatgt cccggccacg
     5281 acgatggatg aacgccaacg cctgcaacac cccatcgtgg acgacgcggg ccaaccgatc
     5341 ccgttcggcg acctcggcct gtcgggccag tgcgcgacgc agttcggcct gctcggtgcg
     5401 cgccacgtcg gcgccgaaac ccaggcaggt cccgagcatg acgatgaggc cggcgttcga
     5461 caacggttcg gaatcgaact gccccacctg cgcgatcatc acggccgtga tgacgaggga
     5521 ggcgatcaac ccgccccgcc agccgaggat gagggcgatc gccacgacgg ggacgttctg
     5581 ccagacgccc gggatcgtcg tgtcaccggt cgtggccgac agcggggtgt cgaaccaccg
     5641 cgtcgacatc acggcggccg aggcgagcac catttccatc acgtggatgc gcaggtcgcg
     5701 gcggcgcgag acatacatgt atgccgtcca caccgaaagc acgccgatca ccatgagcgc
     5761 gaggtccagt cgagccatct cggcatgccg gacccagatg ctccacaggg catacacgag
     5821 ggcaagcacc cggaagacgc gcaaggctcc ccagagggcg ctgaggacga gcgagtcgtc
     5881 gtccgtcgcc ggtgtgctct ggggggtcat ggtgcgaaac taccgcgcga caggctcagc
     5941 tggcccgaag gcgaccctgc tcatccgaac tcgggccgtc gtcgtccacc gaacccgtcg
     6001 atgtagcgga gtcgggggcc tcgactgctt cgtgggtctc caacggttcc tgaggatgag
     6061 ttgccctggt cttggccgac tcggtgacgg cgcgagccgc ttcggtggca gccttcgcgg
     6121 cctccgccgc ggtcttctgg atctcctggg ccgtcttttg gatctcctgc gccttggcca
     6181 cggccgtctg cacggtgtcc ttgagaacgt tcttcttcag cgacgtcgca tagacgtcga
     6241 cgtactcctg gccggacaac agcatgagct cgtacatgat ctcgtcggtg atcgaccgca
     6301 gcacgaaccg gtcgttctcc atgccttcgt agcggctgaa atccagcggt gccccgatcc
     6361 ggacaccgat ccgcatgatc ttcggcagct tctgcccggt gggctgcgcc ttgtcggtgc
     6421 cgatcatcgc caccggaatg acggggcaac ccgcctccag cgccattcgc gcgatgccgg
     6481 tcttgccctt gtagagccgt ccgtcaggtg agcgggtgcc ctcggggtag atgccgaaga
     6541 gttcgtcccg gcgcagcacc ttgagccccg aactgatcgc cgcctcgctg gcccggcccc
     6601 ccgaccggtc gatcgggatc tggccgacgc ccttgaagaa ccacgcggtc agacgaccct
     6661 tgacgcccga accggtgaag tactccatct tggccgggaa ggtgatccgc ctcgacatga
     6721 cgatgggcag gaagatcgag tcggagaagg acaggtggtt gctggccagg atcgccgccc
     6781 cgcgctcggg gatatgctcc gcgccctcga cccacggccg gaacaacagc ttgaggatcg
     6841 ggcccagcac gaccgtcttg aggaaccagt agaacaccgc gggagccctt cctgtgtggc
     6901 ctgcagcact ctagagcacc ggtggcccgg ccggagagcc ccactggccc cgcaggcgtg
     6961 gttcgagtct gccggagcgt gccggggact gatactaggt gggtgaacga cgaggatgcc
     7021 ctcgaggcgg acgaccaagg cagatccgag cccgcgggcg gccctgagga gacttctgcg
     7081 acggcgctcc ccccgttgaa cgagatcgac gccgccttcg cctccatcgt cgcgcattgg
     7141 gacgacccga cacctgagcc gctcgctgac cgcgccgctg acgacgccgc tggcgacgcg
     7201 gcggacctca ccgcacccgc acccctcgcc ccggcacccc tccccccggc ataccccgcc
     7261 accgcacacg tcgccgtgcc cgtgggcgac gccccggacg ccaccactga tcacttttcg
     7321 gccgcagagg acgccgatga ggagcggctc ttcggctggc ggggccacac cccgcctccg
     7381 gaggacgagc acttccaacc gccgccgccg aacctgccgc cggtgcacga cgcnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagggcgt tcccgctgga gtactgctcc
     7561 cagcaccccc ggttgccgca ttcgcagcgc agcccgtcag gaacgacctg catgtggccg
     7621 aactcgcccg cgatgccgaa gcggccacgc tcgacccggc cgccgagcac gatcccgcca
     7681 ccgataccgg tcccgaggtt gaccatgacc agatggctct ccccccggcc ggcgccgaac
     7741 cgccactcgg cccaactgga ggcattggca tcgttgtcga ccgtgatggg cagcccgatc
     7801 cggtcgaaca gggcgtcgcg cagcggctcc tgacgccagg acaggtgcgg cgcgaagacg
     7861 acggtggcgc gatcagcccc gacgaacccg gcggctccga tgccgacggc gctcacctgg
     7921 cctgggccca cctgggcgat caggtcttcg acgacaccca cgatggtgct ctcgacgacc
     7981 tccggtgagg tcgatcgatg aggggtgtcg gcgcgcgcct tcgcgaggat cacgccatcg
     8041 acgtcaacca cgcccccggc caccttggtg ccaccgatgt cgatcccgat cgtgtatgcc
     8101 gcgctcacac cggctcctgg ggttgctcgc cgcccgtcgg cgcgggcgga ggggtctcgg
     8161 tccggcggtt ggccgcgaag ccacgcagcc cctcgctgac caggtcgacg acatcggcga
     8221 tcttgtccag tgtctcagga ctgatcgcct tgacaacatt gaggacccgg cagattgggc
     8281 agatgcggca taccgagtca ccgggcgacg cggcatgcgg gtcgtgccca cactcgggac
     8341 aggcgtgggc tgtcggtgtc tcccccgcgg cccgggatcc ggcggcgaga aggtgcgcct
     8401 ccggcatacc ggcggcgaac acccccaaca agcgcgcggc ttcctcggcg accgagccga
     8461 cccggtcgct cccctcgacc gacacaatca gccctcgacg cgcttcttga gctccttgag
     8521 cgctgcatcg acgatctgct gctcggcctt gcgcttgagc atccccagca tggggatgtt
     8581 gaggtcgacg gccaagcggt aggtcacctt gctgccggcc ttgtcgcggg cgatggtgta
     8641 ggacccgttc atcgccttga gcaccgtggc ccggaccagg ctccacgaga cgacccccgt
     8701 gccgtcatcg gagatgtccc actcatagtc cagcgtgtag gtgtccttga ggacgcctgc
     8761 gtcgagggtg tactccacgc gatcggccca accgtcaccc tcctcggaga gcacggacac
     8821 ccgcttcacc tcggccgccc actcgggata cgcctcgaag tcggcgatga cgtccaggac
     8881 gacaccgggc ttggcgtcca cgtggatgga agactcggtg cgttcggcca tggcaggtag
     8941 gttatcgccc ccgagagccc ccggatcgcc gcgtccggga cggtccgccc gagatcaccc
     9001 ggctgcgagg gtgacgagct cggactgcca gcccgccacg aactgggaca tagtctgccc
     9061 aagcacctcg gtcagcgcgg ctgcggggtc gaccggtcct gccgggctgc tcgacgcgcc
     9121 ggggaccccg ccgacccgcc gcaccagccc gaccaaccct tcgggcccgg cgagggtcgc
     9181 gattcgcgag gcggccagcc aactctcctg gtatgccgcc gccgggtcgc ccgccgtccc
     9241 cgcgaaatcg gtcgcggtcg gcagcgtcgc cggtggaccg gacgcccgga cctgggacaa
     9301 gagccgggcc gccaccacgg tgacgtccat cccttcgtcg cggaaggcca cccactcggc
     9361 gaaaccctcc gagagccaca ggggcaggtc gtgcaaggtc ccggcacgca cggagacatg
     9421 ggtcaactcg tgggtgagca cgacgcggcg ccccgccggt gtcagtcggc ccatgccctg
     9481 aggatccagg acaacccggt cggccactgc ggtcgcgctc ggctcccgag gtccatgcgt
     9541 ggtggccgcg acctgaccgg ccaggggcga ctgttcgctc gacggtgcgc cgttgcccag
     9601 cagcagcgct gcctgatcgg tcgtcgcggg cacgacgacg actgcgggga aggctctccc
     9661 cagtgcggcc gccacccggt cctgcccgac ctcgacctcg gcgagccggt cgcgaagcac
     9721 atcctgcgca actgggccgg ccaccagtgc ccgctcgctg cgagcgacct gaaggggcga
     9781 gaggtcgaac acttcccacc ggtcggcggg gccgacccag cgctggatga gccactgacc
     9841 ggcgacctgg gtgagcgtga ccgtgcgtgc gacccaccga accccccggt cgaacccggg
     9901 aatggcatat cccagccgca tcgacccggt ccacgtctgt gttgtcaacg tctgtgcgtg
     9961 ctgtgactcc tcggctgccg gggcggccga ggtggcctgg gtggccgacc tgcggacctc
    10021 ggaaacgatc acctcgcgca cacccaaggc caccaacccc gcgtatgccg cctcctcagc
    10081 cgatccgtcc ggcccgtgtg ggtcggcgat cgtcgcaagc caggccgttc gatcgcctgt
    10141 ctccaacgcc cgggatcgag cggccatcag cgtggtcaac gccgtccggg ccgcctcgga
    10201 ggcctcggag cccgcgccgg ccacggcatc ggccccggcg ttggccccac tgttcgcccg
    10261 ggtgctggcg gtcgccgtcg tcgagggcgt cgcggcgttg ggcgtgcagc ctgacatcac
    10321 gagggcgacg acgactccgg ccaccagagc ggtcacgacg gcggccccgt gcgctgtcac
    10381 gatggcggtc acgagggagg cgcggcgacc gtcgacatcg gctgctggca gccacctgcc
    10441 ccaggccgat cgcctgccga acgccgtcac ggccctgatg ctatgtcggc acggcgccgg
    10501 ccgccgtcac gtcgggccgc tcagccgacc gcacggccca ctcggcccac ccgaccggag
    10561 tcgaccgtcg aggtgagcgt cgcggcatac ccgctgtcga gcaacccagc cgacacgaac
    10621 aacccgacgg cgcgccgttc cttcgcgtcg agcggtcgct cacctggcgg ccccacggag
    10681 atcgtggcga gggccgtcac cacacagtca tcgcaccgca gcccccgcac ggggcaggtt
    10741 gcgcatgtga cgatcatttc gtcggctcct tctcggatcg gtgggctcgc ttctcgagtc
    10801 cgctccggtt cgttgtcgac ggtagaacca gggtctgaca ccccctggca cgggcgcgcc
    10861 tcccgagggc tgtcagaccc tggttctacg gtcgaagaca tgcacgcagt ccagggcacc
    10921 ctcgatgacc tgggcacccc gctgcacgcg gtgacttttg tcgtcgtcga cctggagacg
    10981 accggaggca gcgccaacga atgcgccatc accgaggtcg gcgcggtcaa ggtccgtggc
    11041 ggcgaggtgc tcggcgagtt ccagaccctc gtcaacccag gctcccccat cccggcgttc
    11101 atccaggtgc tcaccgggat ctccgacggc atggtcgctc aagcgccgcg catcgacacg
    11161 gtgctgcccg ccttcctcga gttcgcccgc ggcagtgtcc tcgtcgcgca caacgccggc
    11221 ttcgacatca ccttcctcaa ggccgctgcg gaggcgaccg gccagtcgtg gccgggtttc
    11281 aaggtcgtcg acaccgtcac cctggcccgc cagttggtga gcaaggacga ggcccccaac
    11341 cggcggttgg gatcgctggc ggcgctcttc ggtgccgaca cgacccctga ccaccgtgca
    11401 ctgcacgacg cccgggcgac ggtcgacgtc ctgcacgcgc tgctcgcccg agtgggcaac
    11461 gtcggggtcc agaccctcga ggagctcgcc agctacagct cgcgggtcac cacggcgcag
    11521 cggcgcaaac ggttcctcgc cgacgacctg ccgaccgctc ccggggtcta cgtcttcaag
    11581 gacgccaagg gacgcccgct ctatgtcggg acctcgcgca acatccgggt gcgcgcccgg
    11641 agctatttca ctgcatccga acaacgcacc cggatggccg agatggtgtc cgtcgcggcc
    11701 tcgatccagc cgatcgtctg ccagaccacg ctcgaagccc aggtgcgcga gttgcggctc
    11761 atcgccgagc acaagccccg ctacaaccgc cgctcgaccc gccccgagcg cgccctctgg
    11821 gtcaagctca ccgccgagcc ctacccccgg ctgtccatcg tccgcgaggt tcgcgacgac
    11881 ctcgcctccg gcgcccggta tgccggcccg ttcggttccc gtgcggctgc cgaggccgcg
    11941 gtggccgccg tgcacgacgt cctccccctg cgccagtgca cgacccgcct gtcgacgaag
    12001 ggcacgggct cggcgtgcgt tctggccgag atgggccgct gcggcgcacc ctgcgagggc
    12061 cgccagagcc tgtcggagta tgccgtgcac gtcgagactg ccgccgctct cctcgtcggc
    12121 gacggccgat ccatggttga tgccctctgc gagcggatga cggtgctggc caaggcggag
    12181 cgcttcgaag acgcgggcgc cctgcgcgat cggctcgggc acctggtgcg cggcctggcc
    12241 cgagcgcagc gtctggcccc gctggcggcc agccccgagc tcgtcgccgc tcgacgcacc
    12301 gacgccggtg cctgggagtt ggtgtgcgtc cgacacgggc ggctggccgg gaccagcctg
    12361 gccccccgcg gtgccgaccc gctcgtctat gtcgagagcc tgcgggccac ggccgaagca
    12421 gtgcccccgc ccgcgggacc ggcgcccgcg gcataccccg aggagacgga gttggtgctg
    12481 cggtggctgg agagccctgg ggtgcgcatc gtcagtctcg atggcgaatg gacgtgtcct
    12541 gtcggtggcg cgggcgcggc gaaggcgacg ttcgacgacc tgcgccgcgg tgcttgagcg
    12601 gtccccgcgg gtgcgccgct aggctgagcg cgtgatcaca gccattgtcc tcgtgacctg
    12661 cgacgccgcc cggatccccg aagtcgccca ggacatcgcg gccctcgacg gcgtgacgga
    12721 ggtctactcc gtcacgggcg acgccgacct cgtggcgatg gtccgggtgc gtgagcacga
    12781 gcagttggcc gacgtcatcg ccgaccggct gtccaaggtc gagggcgtcg ctgcgaccag
    12841 cacccacatc gccttccgca cctacagcag gcacgacctt gaggccgcct tcagtctcgg
    12901 cctcgactag gccgcaggac ccgcatcgaa ccaggggtct gccnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnagctcag cgcccagccg tcaggcgcga gtcgcctcga
    13081 cccaacgctg cacgagagcg tcggcggcgc cggagtccaa ggctgcttcg gcgcgatcca
    13141 tcccggctcg cacgtcggcg tgaaccgcgg cgggggtgcg ccccgagtca ttggcggcgg
    13201 caacagccaa ggctgcaccg gcgttgagaa cgacggcgtc acgcaccggc ccacgcgctc
    13261 cgccgaacac gtcgcgcacc actgcggcgt tgtatgccgc gtcgccgcca cgcagcgcct
    13321 cggttggcga gagggtcaag cccaaccgga tggggtcgag ttggtgctcg acgacgtcac
    13381 ctgaacggac ccaccagacg gtggacgtcg tggccaacgt caactcgtcg agcccatcgt
    13441 cgccgcggaa cactgcggca tcccggcccc gaccggcgaa gacgccggcg atgatcggag
    13501 ccatccgcgc gtcggcgacg ccgacggcgg catacgtcgg ctggcccggg ttggtgagcg
    13561 ggccgaggaa gttgaaggcc gtgggcacac ccaactcccg gcgggtgccg gcgacgtggc
    13621 gcatcgaggg gtggaactgc tgggcgaagc agaaggtgat gccgacctcg tgggccagtg
    13681 cctccacgcg cgctgcgggc agcgtcaggt cgatgcccag ggcttcgagg acatcggccg
    13741 atcccgagcg gctcgaggcc gcacggttgc cgtgcttgac gaccgagact cccgcagcag
    13801 cacacaccat cgcggccatg gtcgagatgt tcaccgtctg ggcgcggtca ccaccggtcc
    13861 cgacgacgtc aaggctgagt tcgggggcct ggatgcgcgt ggcgtgggcg agcatctcgt
    13921 cggccaggcc gcgcatctcc tccacggtct cgcccttggc gtgcagcgcc atgaggaagc
    13981 cggcgatctg ggagggggtc gccccgccgc tcatgatctc gcccatggcc caagcgcagt
    14041 cctgacgtga gagatgacca cgctggagca accgcgagta gacctctggc caggactggg
    14101 tccctacctc gttgttcacc gtcgacccga ggtcagtgcg cggccgtggc gccagccccg
    14161 gccccagcca actgggtcac cgcccgagcc agcgcaaccg ggtcgagggg atgcggcacg
    14221 acggcgtcag cctgcgacca ggccgccagc cacccgtcct ggactcggcc ggtgagcacc
    14281 aacgtcggcg ggcactggaa gatctcgttc ttcaactgac ggcacaggcc catcccaccc
    14341 atcggggtgg cctcgccgtc gaggatgagg acgtcgaaac gccggttgtc gaccgcctcg
    14401 acgacggcag cgtgggtggc gcactccagc caggaggtca cctcgacgtt cttggcgggc
    14461 cgtcggccga ccgcgaggcg cacggcatcg cggaccgtca cgtcgtcgct atagagcaat
    14521 acggagatct ggtggacgga tgcggggacg gccgggctgg ccgagtgcgc ctgcgatgcg
    14581 gtcatgtcgc tcatgctagt cgccacgtgc gcgcgcccgc gcgggccggg ggcggcgcgg
    14641 cggcatacct ctccgttggc gggaagcacc tgtggtgacc aacctcatgg gccccgctga
    14701 gcacgaagtt gactcgtttg gcgattaacc ggggggtcgg tcgcacatgc cgacactcgg
    14761 cacgccataa tggcgcgcgt ggcgacagca tcggcgattc cagcgcaccc agtgagcagc
    14821 gggcatggtc cggcgacccg accgaacatg gtccaggtcg ggaccatggt ctggctctcc
    14881 tccgaactca tgttcttcgc ggggctcttc gcgatctact tccagctgcg gtcggtctcg
    14941 gccgcgctct gggcagagca gaccgcaaag ctcaacgtga ccttcgcgac catcaacacg
    15001 gcgatcctgg tcatctcgtc ggtttggtgc cagatcggcg tcatgaaggc cgaacgcggt
    15061 caggcatcgc gcaccggcaa gctcaccgag atcggcaagt ggggcatgcg cgagtggtac
    15121 gtcgtgacgt atctcttcgg cgcaaccttc atcgccggcc aggtctatga gtacgccaag
    15181 ctggtgtccg agaacgtgac gctctcctcc gaccgctacg gctccgtgtt ctacatggcg
    15241 accggcttcc acggtctgca cgtcaccggc ggcctcatcg cgttcctgct catcatcggg
    15301 cgcaccttca cgacccgccg gtacacccac acccaagcgg tgggcgcggt cgtcacctct
    15361 tactactggc acttcgtcga tgtcgtgtgg atcgcgctgt tcgccgtgat ctacctgctc
    15421 aagtgagcat cgacaccccc cgacccgcga gaacccacga caggacgaca tcgtgaacgc
    15481 cttggccgca cgtcgccgcc acccagccgc catcgccatc ctgctgttcc tcgggctggc
    15541 cctggtcggg acggcatact cgctgcttgc tcccaagccc gcccaggcgg ccgtcgcgac
    15601 ggccgccgac gcggagctcg gcaagcagtt gttcctcgcc aactgcgcga cctgccacgg
    15661 tgtcaacgcc gagggcacca agtccggtcc gacgctcatg ggggtcggcg ccgcgtcagt
    15721 ggacttccag gtcggcacgg ggcgtatgcc gctcgcgggc cccaacgtcc aggcgccgcg
    15781 gatcgacgaa gtcaagttca ccgaggagca gatcgcggcc atggcggcct acgtcgcctc
    15841 gttggctccc ggccccgcga tccccgacgc caaactgacc tcgggtgagg gcggcgacgt
    15901 cgccctcggc ggcgagatct accgggtcaa ctgcgcgatg tgccacaact tcgccggatc
    15961 cggcggcgcg ctcacccgcg gcaagtacgc cccgtcgctc tacggcgttg acggcaagta
    16021 cgtctacgag gcgatgatca ccggcccgca gtcgatgccg gtcttcaacg acaccaacct
    16081 caacccggag gccaaggcct cggtcatcgc ctacctcaag gcgacccagg ccgagccgaa
    16141 cgtcggtgga atgaccttgg gcaacctcgg cccggtgtcc gaaggcatgt tcgtttggat
    16201 cttcggtctc ctgctcatga tcgcctgcgc cttctggctc ggccagaagg ccgcgtgagc
    16261 cccgcctacc ccacgacgac aggatcgatg acctcatgag tgaccaggtc acccccacca
    16321 gcgggtcggc gagcggcgcc ggcaccgtca gcctcgatga ggggcaccac gtgggcaccg
    16381 gcggtctgcc ggcgacgttc gagaaccccg ggctgccacc gcacgtccac cgcgccgctg
    16441 acgacgaccc caaggcggcc aagcgcgccg agcgtcaggt cgcggtgctg ttcacgctgt
    16501 cgatcctcgg cacgctgggg ttcatcgtca gctacttcgc cattgaccgg atgacttcgg
    16561 tcttcctgcc cttcatcggg ctgaccaacc tgctcaacgt catgctcggt gtgtgcctgg
    16621 gcatcgccct gctgggcatc ggtttcggcg ccgtccactg ggccaagacc ctcatgtccg
    16681 acgaggaggt cgtcgaggag cggcacgcga tcgcctcctc cgacgcggca cgtgctgact
    16741 tcgtcggtgc cctggctgcg ggcgcagagt ccgcccagtt ggctcgtcgc ccactcatca
    16801 aggcgaccgc aggcgcggcc ctcgggttgt tcgccctgcc cttgggtgtc cagctcgccg
    16861 ggtcgatggg taaccccacg aaggctctgg aagagctcaa gcagacgatc tgggacgttc
    16921 ccaaggatgg caagccgatc cggctggtcc gcgaccccga gctcaccccc atcaaggctt
    16981 ccgatgtgac gatcggctcg gtcttccacg tgatgccgga gacgctcaag gagctgcgcg
    17041 agcacaacgt caaggaaggc cactccgagt ggcacggcgt gctggaggcc aaggccaagg
    17101 ccgccgtcct gctcatgcgc ctcaatccgt ccgagttcaa ggacgacgtc ctgggccgca
    17161 aggcccgcga ctggggcttc cagggcatcg tcgcctactc caagatctgc acccacgtcg
    17221 ggtgcccggt gggcctttac gagcagcaga cccaccacct gctctgcccg tgccaccagt
    17281 cgaccttcga cgtcacccgt gactgcgagg tgatcttcgg ccccgccaag cgtccgctgc
    17341 cgcaactgaa gatcaccgtg gacgacgagg gctacctcgt cgccccgcag ggcttcgccg
    17401 agcctgtcgg cccgagctac tgggagcgcc tcaagtgagc accactgcct ccgcgcgcac
    17461 cgctgcagac ctgcgcgccg ccgacgccaa gggccgcacc ggctccgaga gcaaggccgg
    17521 cgccgcaggc gtgtgggtcg acgagcgcac cggcctcgcc aagggcgcgg catacatgat
    17581 gaagaaggtc ttccccgatc actggagctt catgctcggt gaagtggcga tgtactcgat
    17641 gatcatctgc ctgctgaccg gtgtcttcct gtcgttctgg ttcgtcccca gcgccggcca
    17701 gatcccctac aacggctcct acctgccgtt gcagggcgtg atcatgtccg aggcatacaa
    17761 ctcgacgctg gcgatctcgt tcgacatccg tggcggtctg ctgatccggc agatccacca
    17821 ctgggccgcc ctgatgttct gtgtcgcgat cacggtgcac atgctgcgcg tgttcctgac
    17881 cggcgcgttc cgcaagccgc gcgagctcaa ctgggtcatc ggctcgatcc tgtcgatgct
    17941 ggccatcgtc gaaggcttcg ccggctactc gctgccggac gacctgctct cgggcaccgg
    18001 tatccgcgcc gccgaaggct tcatccgctc gatcccgctg gtggggtcct acatctccta
    18061 cctgctcttc ggcggcacct tccccggcga ggcgatcatt ccgcggctct actcggtgca
    18121 cgtcctgctg ctgccggccg tgctcatcgg cctgttcacc gcgcacatcg tcctggtcat
    18181 ggtccacaag cacacccagt tccccggccc cggccgcacc aacgagaacg tcgtcggctt
    18241 cccgctgatg ccggtctaca ccgccaaggc tggtggcttc ttcttcatgg tcttcggcgt
    18301 cctggcgatc atctcggcgc tggtgacgat caacccgatc tgggcctacg gtccttacga
    18361 cccgtctccc gtcacggccg gttcgcagcc tgactggtac atgggcttcg ccgacggcgc
    18421 cctgcgcctc ctgccgggtt tcctcgaatt cgagttgttc ggtttcacct ggagcatgaa
    18481 cgtcttcctc ggcgccgtgg ccctcatccc gttgatgtac acggccgcgg gtgtctaccc
    18541 gtggatcgag gcctgggtga ctggcgataa gcgcgagcac cacctgctcg accgcccgcg
    18601 caacgcgccg acccgcactg cgatcggcat ggccgcgttc tcgttctacg tcgtgctgat
    18661 gttcgccgcg ggcaacgaca tcatggccat ccgcctgcag ttgtcgatca acgacgtcac
    18721 catcctcttc cggatgctcg tgttcgtcgt cccggtcatc gtcttctggg tcacgcgacg
    18781 gatctgctgg tcgctgcagc gccacgaccg cgagaaggtc ctgcacggtc gcgagaccgg
    18841 cacgatcatc cggaccgccg agggcaagtt cttcgagcgg cacgaggaca tcgacgcctt
    18901 cgagcggtgg ccgctggttg ctttcgagtc ccccgcacct ctggcgctga cggacacggc
    18961 cgacgccaac ggtgtgtcct ccaaggatgc gaagaaggca cgtcgccgcg tcgtgtggtc
    19021 gaacttctac ttcaagaccc gcgtcgagcc ggtcaccccg accgagttgg cagccgctca
    19081 ccaccacggc gagcacgagg ccatctccgg tggatcggct ccggccgcga tcaagcacga
    19141 caagcacccg cactga
//
LOCUS       DAORPW010000033        19125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_500_length_19125_cov_677.976770, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000033 DAORPW010000000
VERSION     DAORPW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 19125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19125
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_500_length_19125_cov_677.976770"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..>1573)
                     /locus_tag="PLB86_04595"
     CDS             complement(152..>1573)
                     /locus_tag="PLB86_04595"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRC64446.1"
                     /translation="SNSYNGTSYPVSSPAPTAMTGDPGHEFADVLEQLAGAGATYPPG
                     GPYPPITMTGFASNYATTTDEDLPPPTADHIGDVMMEFDTPTQMPTSYALATSFAICD
                     HWFCSIPGPTWPNRFFVHGASSAGLEHAPTTAQIVEWETVAGFVLPHGSIYDALTANK
                     LQWRLYNDNTDAYSDNPQNGSLFGALPQVTALKNVTLLEVNSLTHFASDLQGPYPYQY
                     TFIEPNYGDVTSNTYVGGSSQHPMDDIYGGEALIKGVYEAIRNSPLWETSLLIITYDE
                     HGGFYDSVAPPPAAPPADGSGTAYNDTGFAFDLLGVRVPAIVVSPLIAAGTVDHTVYD
                     HTSALATVERLFGFGALTARDAAANDLRHLFTQTTARTDCPTTLPAPVARMGRVLPDG
                     ASEAAAAAEDDLPLEDSGNLPAFLATARKAAYDTYATTPQERAIVDEEYAAITTRGEA
                     KAFVARVMAKATLVRDSQPRPAM"
     assembly_gap    1574..1673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1961..>2923
                     /locus_tag="PLB86_04600"
     CDS             1961..>2923
                     /locus_tag="PLB86_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC64447.1"
                     /translation="MSTVPRLPVPASTDADEVYAAYLAWAEAQGLTLYPHQDEAIIEV
                     LSGAHVVLATPTGSGKTLVATAAHLAALAADRVSFYTAPIKALVNEKFFALCDIFGAD
                     QVGLLTGDASVNPDAPVICCTAEVLANIALREGAAADIGLVVMDEFHYYGDPQRGWAW
                     QVPLLELTDVQFVLMSATLGDMSKIVADLERRTGRPVAQVTTAERPVPLTYTWSLTPL
                     AEVVEEIVTTRRAPVYVVHPSQAAAAEHAQSLRGLTLLTHAERDEIAERIGPVRFASG
                     FGKVLSALLRKGIGVHHAGMLPRYRRIVEQLAQAGLLKVICGTDT"
     assembly_gap    2924..3023
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3024..4668
                     /locus_tag="PLB86_04605"
     CDS             <3024..4668
                     /locus_tag="PLB86_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HRC64448.1"
                     /translation="VEQLAQAGLLKVICGTDTLGVGINVPIRTVLLTRLVKFDGTRER
                     LLRAREFHQISGRAGRAGYDTVGEVVVQAPEHLIENARRVAKAGDDPIKLKRVQRVKP
                     EAGQIVWNEATFDKLVAAAPEALASRMRVDNAMILNVIARPGDAVRSLSRLTRDNHED
                     AGGRARLARRAIRLGRSLLDSGVVVRLPEPDAQGRTLALTVELPEDFALNQPLAHFAL
                     AALELLDPAAPTHALDVVSVIESVLDSPRPVLLAQQWEARGEAVAEMKADGMEYEERM
                     DALNEVLWPQPLAELLGAAYESYRQSHPWLPDDALAPKSVVRTMWEQAMSFTDLISRY
                     KLARSEGVVLRYLSDAYRTLRQTVPDAHRDPELEDLVDWLGETIRQTDSSLLDEWEAL
                     THPDGTLNTVHRDAVAPPRPLTANDRAFRAMIRRAMWRRVELAARDDINALYAVERVS
                     AALTEPPSSVVMDTAAWDAALGDYWAEHDEVVLDADARGPAYLSIGPHPDGGRRWAVR
                     QTIADPAGDRDWVIEADVDLDASDAVGELVLTTTALRRL"
     gene            5123..6073
                     /locus_tag="PLB86_04610"
     CDS             5123..6073
                     /locus_tag="PLB86_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64449.1"
                     /translation="MDRVARIAADLDSFHRGRGIHADDFATRVGPHLAQVVFADSDPE
                     PTYGRDLLKRWFLVASQDLPPDLRAVFLAASGLTQPGPKLTDRLGALSLSTGLASRTL
                     VRRLRQADVLVAAKLDSLSYVSEDDNPFARRGWYVERLESTAYLNEERPRFVGVREIL
                     CTLDGLEQICESLSVPRPPGDTSPVDLIVEATEGCEVDTLTRVSPSTWQVGLALPKPL
                     TVGQRHRLGVSIAMPSRAFVKPYNAFVPVRRTRSFAAVVHLPNDGSVVGAWRLDGVPP
                     TAMEDGVPIGDSFDLSAPEPLAAEWTTVRRGLGYGIGWSW"
     gene            complement(6118..7020)
                     /locus_tag="PLB86_04615"
     CDS             complement(6118..7020)
                     /locus_tag="PLB86_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64450.1"
                     /translation="MSMQVLDPELASVVWWGTHTLRVRAHAARGASLCPTRFAPYGHA
                     DPLAQQVNLRCWEDGCMGIVHSIYAATSDRLARRAGSDRQAVADRMRYARTVVASQVA
                     ELDRGRRVARGLPARPTRNDGIAGRINEALDAAAAGPTEARWLVQLFRMIRGYVCRDN
                     RTSTTWPTDAWAAEKCRADGGVRGLGTDAVRQEILDDIHTVLSEAQRVAGRAWVNVSI
                     LHPLMAVVGPLTDEFAQQLPSSDPDPAEQALLRQFMTRYAAYRRRGQHPAGAFRTASL
                     EVFGQEPSGDLRDVIVDLESGLVG"
     gene            complement(7183..8649)
                     /locus_tag="PLB86_04620"
     CDS             complement(7183..8649)
                     /locus_tag="PLB86_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64451.1"
                     /translation="MSTAASDHVTVNRLGDTFVVGEDPGAAAGLVVLPVKGGTFVVDP
                     INPRGPGQLRLQGPFDLELVETLFGTAVAAAVDVRLDPPERAVVAMAQSHERRILGRL
                     GALRWLQRASPLALDDRLLRVEGLLLEASIADVLDVDEGSESLHDWAPSLVAWAHRCV
                     SPDLREPVSPEFLDLVADGLAEDSAGSPWSPESADHSGQDAEARAELAAYLRAGLARA
                     RADAPDAPGLPCAPIDALRPQTRAATTSSRNGQLHGSATVDWARVPRGLTLQIEDGAS
                     WRVDTTPGNAHVSVVVTAPTADPTLRLLDVAGQPGRSGVTPALQATLTHPEWMLPLAV
                     VELTLRPETDCWLGVEALGPAVLELIADLDPTAFHMDLHVPGAGRRPRNGAAASIDQA
                     TRWAVRGVASVRLAAAYRAESRAEIHRGACDAFALAGSLFKASADPRDQLAAEQCRAA
                     VRLLGRPHAHSHGPEVIDPEPELTVAERWYAAHLAAAH"
     gene            complement(8646..10067)
                     /locus_tag="PLB86_04625"
     CDS             complement(8646..10067)
                     /locus_tag="PLB86_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64452.1"
                     /translation="MTGRPTLVELCRAAVVGPAWDHRIRRGGGAKASHVALHLGARLV
                     QANWPAHAGTPPRPREPLTTAQADAARATLDRVVPLISEALDAHQRIVVATLEILDDK
                     GAPTTAAVLSEFCSVRVGQHYATSIGQPLDEADVARKLAERHLDRPEPMAAEEFFQRH
                     HTAYLRLWNQDGKEAIRTHPPSRIGLGHDERPPMIGVPGERSWQLGTDRSVYDRYRLH
                     FSDAAKLRTRRPGREGRAVEDPEHLAQLEIWRACQGYGLARPAHRVILESLARATLDE
                     STPLAQARAIWQRWCATPRRIAEGGPGTRDDDLTAAISAKVRVWAADQIAWSLRKSYG
                     ELSSMDDHVVRAIVQKVWMGLSAVEREEVGVVDSTLINGWVSTALDRGVAQVLHERAR
                     AIDTPPSARRCHRTCELLTERPDLARVLVNPDSDDLARQGACTAYEAAAGERWGTAWR
                     EQALTGTEAAAYVSGLTPLEEVG"
     gene            10304..10927
                     /locus_tag="PLB86_04630"
     CDS             10304..10927
                     /locus_tag="PLB86_04630"
                     /inference="COORDINATES: protein motif:HMM:NF006743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HRC64453.1"
                     /translation="MNLADLADLAVRARDLMAPGRRVVLGIVGPPGVGKSTLAQALVR
                     EVGERAAYLPMDGFHLPQARLRALGTRDRMGAPETFDAAGYADLVDAVVASPGMPLTA
                     PGFDRTVEEPVADGVRIDASARLVVTEGNYLLLPGGDWERARVRMAAVWQLRLADETR
                     RERLLARHIAFGKSPAEALEWMTRVDEPNARLVQSYAARADLIIDVT"
     gene            complement(10965..11900)
                     /locus_tag="PLB86_04635"
     CDS             complement(10965..11900)
                     /locus_tag="PLB86_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRC64454.1"
                     /translation="MQLIVANGVPVSPARAHSPSQELLRVGLVQHAWDADADHLRATL
                     SAGIRLAVGAGARIVFLPELTLSRYPADTRPSGIPAVNAEDLESGPTVTFASELAREH
                     GVYVHASLYERADAGDGRGYNTAVLVAPSGELVARTRKTHIPVTAGYYEDLYFRPGPA
                     DDAYPVVALEDPAVRLGMPTCWDEWFPEVARAYALGGAQVLAYPTAIGSEPDHPEFDT
                     QPLWQHTIIAHAIANGLFVVVPNRTGSEGIITFYGSSFIADPYGRVLVQAPRDSEAVL
                     VADLDLDQGSDWLDLFPFLRTRRPDSYAALIRTQA"
     gene            complement(11953..>13570)
                     /locus_tag="PLB86_04640"
     CDS             complement(11953..>13570)
                     /locus_tag="PLB86_04640"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRC64455.1"
                     /translation="VTGDRITAVGDDADVLALRGSRTRVVQARGATVLPGFQDAHVHA
                     PFAGRNLLRVWLNDLPGRPAYLDAVARFAANHPNEPWVVGGGWAMEDFPNGLARKEDL
                     DAIVPDRPVFLFNRDVHSAWVNSAALLAGGITRDTVDPSDGRIERDPQTGEPTGMLHE
                     GAAYTFNDRVVPPPSRLEWEEAILTAQEHLHALGITGWQDAWVTPSTLAAYRSLAADG
                     RLTARVVGALWWDRHRGLDQIADLCSQREAATGNHADSGVPGFHPTSVKIMVDGVLET
                     YTGALLEPYCDGCGGHTDNRGLLYVDPELLAEAVTALDAAGFQVHQHAIGDRAARTAL
                     DAIAAARTANGRNDRRHHIAHLQVLQRSDIPRFSALGVTANAQAYWAKNDPQMEVLTT
                     PFLGPDRTALQYPFASLAAAGTRLAMGSDWAVTTADPLAQIEVATTRSDPADRELAAF
                     LPHERLTLDAALDAFTAGSAWVNHDDDGGAIRVGARADLAVLDADLFGTDLRIGTRAP
                     IGGGPPHSLQGVSDASVVMTVAAGRVVYEA"
     assembly_gap    13571..13670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13805..15250)
                     /locus_tag="PLB86_04645"
     CDS             complement(13805..15250)
                     /locus_tag="PLB86_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HRC64456.1"
                     /translation="MSPSLDNELAEPRQPASIELHSIDYVPDTERHGKVTHLGAIWFV
                     SNINLTAMATGVTALSIGAGLVWTLIATILGSLFGTFFMAFHSAQGPQLGLPQLVQSR
                     PQFGYLGAAVTVWVFALINYIAYNTSDALLSGDAFHELFGINTNVGYFIAALIAAVIA
                     LFGYHWIHRVNRVLTWPLIALMVTLSVAALKDGRLTAETWALGDFQPAPFMTVFVIVA
                     GFQLGWAPYVSDYSRYLPASVGVRSTFWWTYLPSAVSGLWVFALGSIMYAAAGPDATP
                     VEAFRSAADSLINGFGWVAVFALLLGLLSVMAINQYGGMMSMISIRDSIRPVAPSRRI
                     RVIGIGIMFITVWLIAQFVGIERFNSFYGNVLIFLAYAFTPWTSINLVDYFFVRRGNY
                     SIQEMFNPNGMYGRWGWRGQTAYLGALVIMVPFMVTAPFTGSFAAALGNVDYSMFVGL
                     PVAAVIYLVLCRSLDLTAEREVVAAEGLVHR"
     gene            15492..>15821
                     /locus_tag="PLB86_04650"
     CDS             15492..>15821
                     /locus_tag="PLB86_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRC64457.1"
                     /translation="MGGRRPRLSRDDIALTALDILDTEGLSALTMRHLAARLGVRAAS
                     LYNHVSGYDEIIDLIQDLVNQEIDLDTLRAAEFADGLMGFARSYRDAFLRHPHALPHL
                     VRRQMASP"
     assembly_gap    15822..15921
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15922..17267
                     /locus_tag="PLB86_04655"
     CDS             <15922..17267
                     /locus_tag="PLB86_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC64458.1"
                     /translation="PTALPARVRRGYALGGVATGSFGTVPGLMLLPYLTERLGVPAAL
                     AGVIVFAPKAWDVILNPIAGRISDHSTHPDGPRRPFLLRAGLALAVAFALLFAGPASP
                     QALAAGWVAVLFLACATAYAFFQVPYVAMPAELTDSPRERTRIMSLRVAVLALAILIS
                     GGLAPMVRTMVGPERGYAVMGLFVAVLIAVGALGAWWGTRGAPVRAVAAAPGGLRSQL
                     ALVAGNPSFRALILTFIVQALATGAMLAGVDYVARHVLRDPGAGSVLFVCFVAPAIVC
                     TPLWERLAARLGKRRAYAAASLTLAAGAAALVAARVMPAALVYVAVAVVGVGYAGAQM
                     LPLSMLPDVAAADAAESGENRVGVFTGVWTAGETLGMALGPFVFGLVLAAGGYVSSTD
                     STAAQPDSAVMAIVLGFSVLPALLTLASLFFLRGYSPPMPRGFATYGPESEVDRP"
     gene            17264..18697
                     /locus_tag="PLB86_04660"
     CDS             17264..18697
                     /locus_tag="PLB86_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRC64459.1"
                     /translation="MTLTADEVRARLLTLREHDLPTHGGRTLAYVYDSGRPDVDAVAR
                     EAVAAFAGTNGLDPSVFPSVATMERGVVDFLAGLLSAPEGYAGTVTSGGTESCLLAVL
                     AARDSRPDVTRPQIVAPTTAHAAFHKAAHLFGLELTLVDVDPVTFRADPAAMAAAVTD
                     RTVLVVVSAPSYAHGVVDPVGPIAAMAAERGIRCHVDACIGGFVLPFLADESARGVAQ
                     GRPAWSFEVPGVTSISADLHKYGYAPKGVSALVHRSMALRHSQFWATADWPGYGVVNS
                     TLQSTKSAGPLAAAWTTIELLGEQGYAALARQARDGALALAAEVGGIPGLRVLVPPDS
                     SLLAVVTDDSCDPYTVCDELLERGWFAQPQLPFRGGPASLHLTISAATTPQLPEFVGA
                     LADSVAAAQVAGPVAVDPAMVGLLATLDPATFDEATLGALLAAAGLTGAGGALQLPRR
                     MAAIQALLAACPPALRRVLLTAVVERL"
     gene            18818..>19125
                     /locus_tag="PLB86_04665"
     CDS             18818..>19125
                     /locus_tag="PLB86_04665"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851908.1"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HRC64460.1"
                     /translation="MNSDYELDTTRRDAPLRISARGRAVLANPMTNRGTAFTVQERRY
                     LGLVGLMPSEVTPIGNQVRRVYQQYHSARTPFAKNNYLASLRDRNEVLFYRLLADHIE
                     "
BASE COUNT         2716 a         6461 c         6624 g         2924 t
ORIGIN      
        1 gggcctgggc tctgggcgcg ggcatgacgg tgtgcgtttg agggggtgat caccccctca
       61 aacgcactcc gtcgagcact ctgccgcggc gctgctcgga ccaaccggca gcgggcccac
      121 aacagcagtg ggccgaccag cggcagcgga cctacatcgc cgggcgcggc tgtgagtcgc
      181 ggacgagggt cgccttggcc atcaccctgg cgacgaacgc cttcgcctcc ccacgtgtgg
      241 tgatcgcggc atactcctcg tcgacgatcg cccgctcctg gggcgtggtc gcataggtgt
      301 cgtatgccgc cttgcgagcc gtcgcgagga aggccggcag gttgccgctg tcctccagag
      361 gcaggtcgtc ctctgccgcg gccgccgcct cgctcgcgcc gtcgggcagg acgcgaccca
      421 tcctggcgac cggcgccggc agcgtcgtcg ggcagtcggt gcgtgccgtg gtctgcgtga
      481 acaggtgccg caggtcgttg gccgccgcgt cgcgggcagt cagcgcgccg aagccgaaca
      541 gtcgctccac cgtggccagc gccgaggtgt ggtcatagac cgtgtggtcg accgtccccg
      601 cggcgatgag gggtgagacg acgatggccg gcacgcgcac gcccagcagg tcgaaggcaa
      661 agcccgtgtc gttgtatgcc gtcccgctcc cgtccgcggg cggggcagcc ggcggcggcg
      721 cgaccgagtc atagaagccg ccatgctcgt cgtaagtgat gatgagcagg ctcgtctccc
      781 agagcgggga gttgcggatc gcctcgtaca cgcccttgat gagcgcctcg ccgccgtaga
      841 tgtcatccat cgggtgctgc gaggacccgc cgacgtaggt attgctggtg acatcgccgt
      901 agttgggctc gatgaacgtg tattggtagg gataggggcc ctgcaggtcg ctggcgaagt
      961 gggtcagcga gttgacttcc aggagggtca cgttcttcag cgcagtcacc tggggaaggg
     1021 cgccgaacag cgaaccgttc tgcgggttgt cgctataggc gtccgtgttg tcgttgtaga
     1081 ggcgccactg gagtttgttg gccgtgagcg cgtcgtagat gctcccgtgg ggcagcacga
     1141 aaccggcaac tgtctcccac tcgacgatct gtgcggtcgt cggagcgtgc tccaggccgg
     1201 ccgacgatgc cccgtggacg aagaaccggt tgggccaggt cggccccggg atcgagcaga
     1261 accagtggtc gcagatggcg aacgaggtcg ccaaggcgta ggacgtcggc atctgggtgg
     1321 gggtgtcgaa ttccatcatg acgtcgccga tgtggtcagc cgtcggcggc gggaggtcct
     1381 cgtccgtcgt ggtggcgtag ttggaggcaa acccggtcat ggtgatcggc ggatacggcc
     1441 cgcctggggg gtaggtcgcg ccagcgccgg ccaattgctc caggacgtca gcgaattcgt
     1501 ggccaggatc gccagtcatc gccgtcgggg ccggtgagct cacggggtag gacgtcccgt
     1561 tgtaggagtt gctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtgatga
     1681 agacgtttgc agtgcgctgt gtagccatgg tggaactcct catcgggtcg gccaatcgcc
     1741 ccgagcgcgg ctgaacggtg ctgggggagg gggagagcac tcgggcccga gtgatgaagc
     1801 gctagctttc acagttgatc acggttcgtg agtgagtgga cccttaccgc gcgaatttcc
     1861 caacgttcct atcgaattcg tcccgcgcgc ccaaggcagc gcgctgcggg cgggctcgag
     1921 cggcataccc ggtgggcgtg ggcctgcgag gatgggcggg atgtccactg tcccgcgcct
     1981 gcccgtcccc gcgtccaccg acgccgacga ggtgtatgcc gcgtacctcg cctgggcgga
     2041 ggcccagggg ctcaccctct atccccacca ggacgaggcg atcatcgagg tgttgtccgg
     2101 ggcgcacgtc gtgctggcga ccccgaccgg gtcgggcaag acgctcgtcg ccacggccgc
     2161 ccacctcgcg gctctcgcgg ccgaccgggt gagtttctac acggcaccga tcaaggcgct
     2221 ggtcaacgag aagttcttcg cgctgtgcga catcttcggc gccgaccagg tggggctgct
     2281 gaccggtgac gcctcggtca accccgacgc gcccgtgatc tgttgcaccg cagaggtgtt
     2341 ggccaacatc gccctgcgtg agggagcggc ggcggacatc gggctggtcg tcatggacga
     2401 gttccactac tacggcgacc cgcagcgggg ttgggcctgg caggtgccgc tgctggagtt
     2461 gaccgacgtc caattcgtgc tgatgtcagc cacattgggc gacatgagca agatcgtcgc
     2521 agacctggag cgacgcaccg gccggccggt ggcgcaggtg acgaccgccg aacgcccggt
     2581 gccgctgacc tacacgtggt cactgacccc actcgcggag gtcgtcgagg agatcgtcac
     2641 cacgcgccgg gcccccgtct acgtcgtcca tccgtcgcag gccgccgccg ccgagcacgc
     2701 ccagtcgctc cgcggcctga ccctgctgac ccacgccgag cgcgacgaga tcgccgagcg
     2761 gatcgggccg gtgcgattcg cgtccgggtt cggcaaggtg ctgtcagcct tgctgcgcaa
     2821 gggaattggc gttcaccacg cggggatgct gccgcgctat cggcggatcg tcgagcaact
     2881 cgcgcaggcc ggcctgctca aggtcatctg cgggaccgac accnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnncgtcgag caactcgcgc aggccggcct gctcaaggtc
     3061 atctgcggga ccgacaccct gggcgtcggc atcaacgtcc cgatccgcac cgtgctgctc
     3121 acccggctgg tgaagttcga cggcacgcgg gaacggctcc ttcgggctcg cgagttccac
     3181 cagatctccg ggcgagccgg ccgcgctggc tacgacacgg tgggtgaggt cgtcgtccag
     3241 gcccccgaac atctcatcga aaacgcccgc cgggtcgcca aggcgggcga cgacccgatc
     3301 aagctcaaac gggtccaacg ggtcaagccc gaggccggcc agatcgtctg gaacgaggcg
     3361 accttcgaca aactcgtcgc ggccgcgccc gaggcactcg cgtcccggat gcgggtcgac
     3421 aacgcgatga tcctcaacgt catcgcccgt ccgggcgacg cggtgcgatc gctgtcgcgg
     3481 ctcacccggg acaaccacga ggacgcgggc gggcgggctc gattggcgcg cagggcgatt
     3541 cggctcgggc gcagcctgct cgactccggg gtcgtcgtcc ggttgcccga gcccgacgcc
     3601 cagggacgca ccctggccct caccgtcgag ctgcccgagg acttcgccct taaccagcct
     3661 ctggcccact tcgccctcgc cgcgctggaa ctgctcgacc cggcggcccc gacccacgcc
     3721 ctcgacgtcg tgtcggtgat cgagtcggtc ctcgactcgc ccaggcccgt gctcctggcc
     3781 cagcagtggg aggcgcgcgg cgaggcggtc gccgagatga aggccgacgg catggagtac
     3841 gaggagcgca tggacgcgct caacgaggtg ctctggccgc agccgctcgc ggagttgctg
     3901 ggggcggcat acgagtccta tcggcagtcc cacccgtggt tgccggacga cgcgctcgcc
     3961 ccgaagtccg tcgtgcggac gatgtgggag caggcgatga gcttcaccga cctcatctcc
     4021 cgctacaagc tggcccggtc cgaaggggtg gtcctgcgct acctgtccga cgcctaccgg
     4081 acgctgcgac agaccgtccc cgacgcccat cgcgaccccg agctcgagga cctcgtcgac
     4141 tggctcggcg agacgatccg ccagaccgac tcgtcgctgc tggatgagtg ggaggcgctc
     4201 acccacccgg acggcacgct caacaccgtc caccgggacg ccgtcgcgcc gccgcgcccg
     4261 ctcaccgcca acgaccgcgc cttccgggcg atgatccgcc gcgcgatgtg gcggcgcgtc
     4321 gagctcgctg cccgcgacga catcaacgcc ctgtatgccg tggagcgcgt ctccgccgcc
     4381 ctcaccgaac cgccgagttc ggtcgtcatg gataccgcgg cttgggacgc ggcgctgggg
     4441 gactactggg ccgagcacga cgaggtggtg ctggacgcgg acgcccgcgg accggcatac
     4501 ctctcgatcg ggccgcaccc cgacggcggg cgccggtggg cggtccggca gaccatcgcc
     4561 gacccggccg gtgaccgcga ctgggtcatt gaggctgacg tcgacctgga cgcgagtgac
     4621 gccgtgggcg agctcgtcct cacgaccact gccctgcgcc gcctctaagg gagccaggcc
     4681 agccccccgc ttcgcctgac cggtcttcgg tgccggaggg tcctttttcg acgcatttct
     4741 ggctttagtt ccctctcgcc tttgccgtcg cacgcgctgg gcccgccatt cgggcgggcc
     4801 tatttcgtcg accccctcct ctcacctgcc catccccaat cgggatcggt tgtcgggcgt
     4861 ccgcgccgct cgacacgcag gggaggaggg gttgcgatgc agtggcattg ctccatcaag
     4921 gacgacttct ggtccgttct cacaagcttc gtcatgttcg cgggttgctg acccacggcg
     4981 accgcaggcg ccgggtgtga attcgcaccc ggcgccctgt tcactttcga gcgcaggttc
     5041 aagccgtggc ctccgtccgg gtatggttcg cctcgtcggt cgacgggagc tggctgacac
     5101 ggaccggcca agggagaacg aggtggatcg ggtcgcacgc attgctgccg atctggacag
     5161 cttccaccgt gggcgcggaa tccatgcgga cgacttcgca accagggtgg gtccgcatct
     5221 cgcacaggtg gtcttcgccg actcggaccc cgaaccgacc tatggccgtg acctgctcaa
     5281 gcgctggttc ctcgtcgcca gccaggacct gccaccggac ctccgcgcgg tcttcctggc
     5341 ggccagcggg ttgacccagc ccgggcccaa actgaccgat cgcctgggcg cgctttccct
     5401 gagcaccggt ctcgcgtccc gcaccctggt gcgtcggctg cgccaggccg atgtgctcgt
     5461 cgccgccaaa ctcgattcgt tgagctacgt ctccgaggac gacaatccct tcgcgcggcg
     5521 agggtggtat gtcgagcggc tggagtccac ggcatacctc aacgaggaac ggccgcgatt
     5581 tgtcggcgtg cgcgagatct tgtgcacgct cgacgggctc gagcagatct gcgagtcgct
     5641 gagcgtgccc cgccctcccg gtgacacgtc acccgtcgac ctcatcgtgg aggcgacgga
     5701 ggggtgcgag gtggacacgt tgacccgggt cagcccgagc acgtggcagg tgggtctggc
     5761 gctgccgaag ccgctgacgg tcgggcaacg ccaccgtctc ggcgtctcga tcgcgatgcc
     5821 gtcacgggct ttcgtcaagc cctacaacgc gttcgtgccg gtgcgacgta cccgcagttt
     5881 cgcggcggtc gtccacctgc cgaacgacgg gtcggtcgtc ggtgcctggc gcctggacgg
     5941 tgtcccgccg acggcgatgg aggacggcgt gccgatcggg gactccttcg acctgtccgc
     6001 gccggagccc ctggccgccg aatggaccac cgtccgtcgt gggttgggct acggcatcgg
     6061 ctggtcctgg taggcggacc ccctcgccga ctcccagtca gcccgacagc cagcccgtca
     6121 gcccaccaag cccgactcta gatcgacgat gacgtcgcgc agatcgccgg acggctcctg
     6181 accgaagacc tcgagtgacg ccgtccggaa ggcgcctgcc gggtgctgcc cgcggcggcg
     6241 gtacgcggca tacctcgtca tgaactgtcg caggagagcc tgctcggcgg ggtcggggtc
     6301 gctgctcggc aactgctggg cgaactcgtc ggtcagtggg ccaaccaccg ccatgagggg
     6361 atgcaggatg ctgacgttga cccaagcacg ccccgccacg cgctgcgcct cggacagcac
     6421 cgtgtggatg tcatcgagga tctcctgacg caccgcatcg gtccccagac cgcgcacgcc
     6481 tccgtcggcc cggcacttct ccgccgccca ggcgtcggtg ggccaggtcg tcgaggtccg
     6541 gttgtcgcgg cagacgtagc cgcgaatcat ccggaacaac tgcaccagcc agcgtgcctc
     6601 ggtcgggccc gcggccgccg cgtcgagggc ctcgttgatc cggccggcga tcccgtcgtt
     6661 gcgggtcggt cgagccggca gcccacgagc aaccctgcgg ccccggtcca actcggccac
     6721 ctgtgatgcg acgacggtgc gggcgtagcg cattcgatcg gcaacggcct gacgatccga
     6781 cccggcccgc cgggccagcc ggtcactggt cgccgcatag atgctgtgca cgatgcccat
     6841 gcagccgtct tcccagcagc gcaggttgac ctgctgcgcc aacgggtcgg cgtgaccgta
     6901 gggggcgaac cgggtcggac agaggcttgc gccgcgcgcg gcatgggcac gcacacgaag
     6961 cgtgtgcgtg ccccaccaca caacgctcgc caactcaggg tccaggacct gcatcgacac
     7021 gggaaacctc cgaaaacacg tgggccgggc atgtcgacgc ggtggtcggc atggccctgt
     7081 catcgtgttg tcaggggtcc acaaggtgaa ggttgtgggc cgatactgtc cggtaattgt
     7141 ccggtccaac tcccggccca ccccctggcc ggcgtcagcc cctcagtgcg cggccgcgag
     7201 gtgcgcggca taccagcgtt cggcgacggt gagctcgggc tcggggtcga tgacctcggg
     7261 gccgtgcgag tgcgcgtggg gtctgccgag gaggcggacc gcggcgcggc attgctccgc
     7321 ggcgagttgg tcgcgagggt cggcggaggc cttgaacagt gacccggcca gggcgaacgc
     7381 gtcgcaggcg ccacggtgga tctcggctcg gctctctgcc cggtatgccg cggcgagacg
     7441 caccgaggcc acgccgcgga cggcccaccg agtggcctga tcgatgctcg ctgcggcgcc
     7501 gttgcggggt cgccggcctg ctccgggcac gtgaaggtcc atgtggaagg cggtggggtc
     7561 cagatcggcg atgagctcga gcaccgctgg gccgagcgcc tcgaccccca gccagcagtc
     7621 ggtctcgggg cgcagggtga gctcgacgac ggccagggga agcatccact cggggtgggt
     7681 cagggtggcc tgcaaggctg gggtcacgcc ggatcgaccc ggttgaccgg cgacgtcgag
     7741 cagtcgcaag gtcgggtccg cagtcggcgc ggtgacgacc accgatacgt gggcattgcc
     7801 gggagtcgtg tcgacccgcc aggaggcgcc gtcctcgatc tgcagggtca gcccccgcgg
     7861 cacccgcgcc cagtcgacgg tcgcggaccc gtgcagttgc ccgttacggg agcttgtggt
     7921 cgcagcgcgc gtctgcgggc gcagcgcgtc gatcggagcg cacggcaggc cgggtgcgtc
     7981 gggtgcgtcg gctcgggctc gcgccagtcc cgcgcgcagg tatgccgcga gctcagctct
     8041 cgcctcggcg tcctggccgc tgtggtctgc cgattcgggc gaccagggcg acccggccga
     8101 gtcctcggcg agcccgtcgg cgaccaagtc gaggaactct gggctcacgg gctcgcgcaa
     8161 gtccggcgac acgcaccgat gcgcccacgc cacgagcgag ggcgcccagt cgtgcaggct
     8221 ctcggacccc tcgtccacat cgagcacatc ggcgatcgac gcttccagga gcagcccttc
     8281 gacccgcaac aggcgatcgt ccaacgccag cggcgaggcc cgctgcagcc agcgcaacgc
     8341 cccgaggcga ccgaggatcc ggcgttcgtg ggattgggcc atcgccacga cggcccgctc
     8401 gggcgggtcg aggcgcacgt cgacagccgc ggccacggcg gtcccgaaca gggtctcgac
     8461 aagctccagg tcgaacgggc cctgaagcct cagctgtccc ggcccgcgag ggttgatcgg
     8521 gtcgaccacg aaggtgccgc ccttgacggg cagaacgacc agcccggcgg cggcgccggg
     8581 gtcctcacca accacgaacg tgtcgcccag ccgattcacg gtgacgtgat cggacgcggc
     8641 tgtgctcacc cgacctcctc cagcggggtg agcccggaca cgtaggcggc cgcttcggtc
     8701 ccggtcaggg cctgctccct ccacgcggtc ccccaccgct caccggccgc cgcttcgtat
     8761 gccgtgcacg ccccctggcg ggcaaggtcg tcggagtccg ggttgaccag gacgcgagcc
     8821 aggtcgggtc gctcggtgag caactcacac gtccgatggc agcgtcgcgc gctcggcggc
     8881 gtgtcgatgg ctcgcgcccg ttcgtgaagc acctgcgcga ccccccggtc gagggcggtg
     8941 gacacccaac cgttgatcaa cgtgctgtcc acgaccccaa cctcctctcg ctcgacggcc
     9001 gacagcccca tccacacctt ttgcacgatg gcgcgcacga cgtggtcgtc catgctcgat
     9061 agctcaccgt aagactttcg cagcgaccac gcgatctgat ctgcggccca cacccgcacc
     9121 ttggccgaga tcgccgcggt gaggtcgtcg tcgcgggtgc ccgggccacc ctcggcgatc
     9181 cgacgcggcg tggcgcacca gcgctgccag atcgcacgtg cctgcgccag cggggtggac
     9241 tcatcgaggg tggccctggc caaactctcg aggatcaccc ggtgggcggg ccgggccagc
     9301 ccgtaaccct gacacgcgcg ccagatctcg agttgggcca ggtgctcggg atcctcgacc
     9361 gcccgtccct cccggcccgg tcgacgggtc cgcaacttgg cggcgtcgct gaagtgcagc
     9421 cggtagcggt catacacgga tcggtcggtc ccgagttgcc aggagcgttc cccaggaacg
     9481 ccgatcatcg gaggccgctc gtcgtggccg aggccaatgc gcgacggcgg atgggtgcgg
     9541 atcgcctcct tcccgtcctg gttccacagc cgcaggtatg ccgtgtggtg ccgttggaag
     9601 aactcctccg cagccatcgg ctcgggccga tcgaggtggc gttcggcgag cttacgtgcg
     9661 acgtcggcct cgtccagcgg ctgcccgatc gaggtcgcgt agtgctgccc gacccggacc
     9721 gagcagaact cgctgagcac ggcggcggtg gtcggcgcgc ccttgtcgtc gaggatctcc
     9781 agggtcgcga ccacaatccg ttgatgggcg tccagggcct cggagatgag gggaacgacc
     9841 cgatcgagcg tcgccctggc ggcgtcggcc tgagcggtcg tcagcggttc gcgcggacgt
     9901 ggcggagtcc ccgcgtgggc cggccagttg gcctggacga gtcgggcgcc gaggtgcaga
     9961 gcgacatggc tcgccttggc acccccgccg cggcggatcc gatggtccca ggccgggccg
    10021 acgacggcag cccggcacag ctcgacgagc gtcgggcggc cggtcacccc ggactcccga
    10081 gaacctcgcg agcatgcgca tcaaccctca tcggcagcac gcctcccctg gccggtcgtc
    10141 acgccgatgt ggctcacggc cggatgagcc cccctgcatg ctcacccgac cgtgttgatg
    10201 ttccgatgtc gggtgccgtg acctcacccg ccatgccatc cctgacggac agaaagaagg
    10261 ggggcgctcc ggacaaaccg gtcggccgtc tactctcgcg agggtgaacc tcgccgatct
    10321 cgccgatctc gctgtccggg cgcgtgatct catggcccca ggtcgccggg tcgtgctcgg
    10381 catcgtcggc ccaccgggcg tggggaagtc gaccctcgcc caagccctcg tccgcgaggt
    10441 cggtgagcgg gcggcatacc tgccgatgga tgggttccac ctgccccagg cgaggttgcg
    10501 cgccttgggc acacgcgacc ggatgggggc gcctgagacg ttcgatgccg cgggttacgc
    10561 ggacctggtc gacgcggtgg tcgccagccc cggtatgccg ctcaccgccc ccggtttcga
    10621 ccgcaccgtc gaggagccgg tcgccgacgg agtgcggatc gatgccagtg cgcggctggt
    10681 ggtgaccgag ggaaactacc tgctgcttcc cggcggcgac tgggagcggg cccgggtgcg
    10741 gatggcggcg gtctggcagt tgcgactggc cgatgagact cggcgggagc ggctgctggc
    10801 gcggcacatc gccttcggca agtcgcccgc cgaggcgctg gagtggatga cgcgcgtcga
    10861 cgagccgaac gctcggctcg tgcagtcgta tgccgcccgc gccgacctca tcatcgacgt
    10921 cacctgaggg gtccagcccc ctcgcagatc aggctgggta cggatcaggc ctgggtacgg
    10981 atcagggcgg cgtaggagtc ggggcggcgg gtgcgtagga aggggaacag gtccaaccag
    11041 tctgagcctt ggtcgagatc gaggtcggcg accaggacgg cctcgctgtc gcggggtgct
    11101 tggacgagga cccgcccata cggatcggcg atgaacgaac tcccgtagaa ggtgatgatc
    11161 ccctcgctgc cggtgcggtt gggcacgacg acgaacaacc cgttggcaat cgcgtgggcg
    11221 atgatcgtgt gctgccacaa cggctgcgtg tcgaactcgg ggtgatcggg ctctgaccca
    11281 atcgccgtcg ggtaggcgag gacttgggcg ccgccgagtg cgtaggcccg cgccacctcc
    11341 gggaaccact cgtcccagca ggtcggcatc cccagccgga cggcgggatc ctcaagcgcc
    11401 accacgggat aggcgtcatc tgccggtccc ggccggaagt acaggtcctc gtagtagccg
    11461 gcggtgacgg ggatgtgggt cttgcgggtg cgggccacca actccccgga cggagcaacg
    11521 aggacggcag tgttgtagcc acggccatcg ccggcgtcgg cgcgttcgta gaggctcgcg
    11581 tggacgtaca cgccgtgctc gcgggccagc tcggacgcga acgtcaccgt cggcccggac
    11641 tcgaggtctt cggcgttcac ggctggtatg ccgctcgggc gggtgtcggc ggggtaacgc
    11701 gagagggtca gctcggggag gaagacgatc cgtgcgcccg cgcccaccgc gaggcgaatg
    11761 ccagcactga gcgtggcgcg caggtggtcc gcatcggcat cccatgcgtg ctggacgagg
    11821 ccgacgcgaa gcagttcttg gctcggagag tgagcgcggg cgggagagac ggggaccccg
    11881 ttggcgacga tgagttgcat ggtggacctt tcgcaggtgg gaagtgtcga gagtgttcgg
    11941 cggttgccgc ggtcaggcct cgtagacgac tcgcccggcc gcgaccgtca tgacgacgct
    12001 ggcgtcgctc acgccttgca gagagtgcgg cgggccgccg ccgatgggcg ccctggtgcc
    12061 gatgcgcaga tccgtcccga agaggtcggc gtcgaggacg gcgagatcgg cgcgggctcc
    12121 gacgcggatc gccccaccgt catcgtcatg gttgacccac gccgaaccgg cggtgaaggc
    12181 atccagggcc gcgtcgaggg tgagtcgctc gtgtggcagg aaggcggcga gctcgcggtc
    12241 tgcggggtct gatcgggtgg tggccacctc gatctgggcg agcgggtcgg ccgtcgtcac
    12301 ggcccagtcg ctgcccatcg cgagccgagt gcccgcggcg gcgaggctgg cgaacggata
    12361 ctgcagggcc gtgcgatccg gcccaaggaa gggggtcgtg agcacctcca tctgagggtc
    12421 gttcttggcc cagtaggcct gggcgttggc cgtgaccccc aacgcgctga accgggggat
    12481 atcgctgcgc tgcagcacct ggaggtgggc gatgtggtgg cgacgatcgt tgcggccgtt
    12541 ggccgttcgc gctgcggcga tggcgtccaa cgcagtccgg gcagcccggt cgccgatggc
    12601 gtgttggtgg acctggaaac ctgccgcgtc gagcgcggtc accgcctcgg cgagcagttc
    12661 cgggtcgaca tagagcaggc cccggttgtc ggtgtgcccg ccacatccgt cgcagtacgg
    12721 ctccagcagg gctccggtgt aggtctccaa gaccccgtca accatgatct tcacgctcgt
    12781 cgggtggaac cctggtacgc ctgagtcggc atggttcccg gtggcggcct cccgctggct
    12841 gcagaggtcg gcgatctggt ccaggccgcg gtggcggtcc caccacagtg ccccgacgac
    12901 gcgcgcggtg agccgaccgt ccgccgcgag gctgcggtat gccgcgagcg tcgagggcgt
    12961 gacccacgcg tcctgccatc cggtgatgcc cagcgcgtgc aggtgctcct gcgccgtcag
    13021 aatcgcctcc tcccactcga ggcgactcgg gggagggacc acgcgatcgt tgaacgtgta
    13081 ggccgcgccc tcgtggagca tgccggtcgg ttccccggtc tgtgggtcgc gctcgatgcg
    13141 cccgtcgctc ggatcgacgg tgtcccgagt gatgcccccg gcgagcagcg ccgcgctgtt
    13201 cacccaggcg gagtggacgt cacggttgaa gaggaagacc ggtcggtccg ggacgatcgc
    13261 gtcgaggtcc tccttgcggg ccagaccatt cgggaagtcc tccatggccc agccgccacc
    13321 gaccacccac ggctcgttcg gatggttggc tgcaaaccgg gcgacggcgt cgagataggc
    13381 cgggcggccg ggcaggtcgt tgagccagac ccgaaggaga ttgcgtccgg cgaagggcgc
    13441 gtggacgtgg gcgtcctgga agcctggcag gaccgtggca ccccgcgcct gcacgactcg
    13501 ggtccgcgac ccccggaggg cgaggacgtc cgcgtcgtcc ccgaccgcgg tgatgcggtc
    13561 gccggtgacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gagggcgagg
    13681 acgtccgcgt cgtccccgac cgcggtgatg cggtcgccgg tgacggcgat cgcggcggca
    13741 taccgaccgg gggcgaggcg agcacgcgct cccacgacga gcaggtccgg tgccggggtc
    13801 gtggtcacct gtggaccagt ccctcggctg ccacaacctc gcgttcggcc gtgaggtcga
    13861 ggctgcggca gagcacgagg tagatcactg cggcgaccgg caggccgacg aacatggagt
    13921 agtcgacgtt ccccagggcg gccgcgaagc tgccggtgaa gggcgcagtg accatgaacg
    13981 ggaccatgat gaccagcgcg ccgaggtatg ccgtctggcc gcgccacccc cagcggccgt
    14041 acatcccgtt ggggttgaac atctcctgga tcgagtaatt gccccgccgg acgaagaagt
    14101 agtcgaccag gttgatcgag gtccacgggg tgaaggcgta ggccaggaag atgaggacgt
    14161 tgccgtagaa gctgttgaac cgctcgatgc cgacgaactg ggcgatgagc cagacggtga
    14221 tgaacatgat gccgatgccg atgacccgga tgcggcggct cggcgctacc ggcctgatgg
    14281 agtcgcggat cgagatcatg gacatcattc cgccgtactg gttgatcgcc atgaccgaca
    14341 gtaggccgag gagtagagcg aacacggcga cccagccgaa gccgttgatc aggctgtcag
    14401 cggcagaccg gaaggcctcg acgggcgtcg cgtccgggcc ggcggcggca tacatgatgg
    14461 acccgagcgc gaagacccac agacctgaga cggcgctcgg caggtacgtc caccagaagg
    14521 tggagcggac gccgacgctc gcgggcaggt agcgggagta gtcggacacg tagggcgccc
    14581 agccgagttg gaagccggcg acgatgacga agacggtcat gaacggtgct ggctggaagt
    14641 cgccgagcgc ccaggtctct gcggtgagtc gaccatcctt gagggcagcg accgaaaggg
    14701 tgaccatcag ggcgatgagt ggccaggtga ggacccggtt gacccgatgg atccagtggt
    14761 aaccgaacag ggcgatgacc gcagcaatca aggcagcgat gaagtacccg acgttggtgt
    14821 tgatgccgaa cagttcatgg aaggcgtctc cggagagcag cgcatcggac gtgttgtagg
    14881 cgatgtagtt gatgagggcg aagacccaca cggtgacggc ggctccgagg tacccgaatt
    14941 gcgggcgaga ttggaccaac tgcggaagcc ccagctgtgg accttgcgct gagtggaagg
    15001 ccatgaagaa ggtgccgaac agcgacccga ggatcgtcgc gatgagggtc cagacgaggc
    15061 cggcgccgat cgagagggcc gtgacgccgg tggccatcgc ggtgaggttg atgttgctga
    15121 cgaaccagat ggcgccgaga tgggtgacct tcccatgccg ctcggtgtcg gggacatagt
    15181 cgatcgagtg cagttcgatc gatgccggct ggcggggctc ggccagttcg ttgtccaagg
    15241 aaggtgacat gggttcctcc gtcgcggggc cctgggcgcc aagcggggca tcccgctccg
    15301 atgcgccaaa ctaacaacgt tagtttcgcg caacgtacac ccgggtgagg tgcgccacaa
    15361 gaggcggcga cagcaggggg ccctggggca tgcggcgacg ggaggtactg tcgggcacgg
    15421 ctgatcccag cccgacccga cgatcccgtt cggtgctcgg ctattcgggg ggccttgatc
    15481 gaggaggaag catgggcggc cggcgtccgc ggctgagccg tgacgatatt gcgctgaccg
    15541 ccctggacat cctggacacc gaagggttgt ccgcgctgac catgcggcac ctggccgccc
    15601 gcctgggcgt gcgagccgcg tcgctctaca accacgtgtc cggctacgac gagatcatcg
    15661 acctcatcca agacctggtg aaccaagaga tcgacctgga cacccttcgt gccgccgagt
    15721 ttgccgacgg gctcatgggc ttcgctcgca gctaccgcga cgccttcctg cgccacccgc
    15781 atgccctgcc acacctggtc cgccgccaga tggctagccc gnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15901 nnnnnnnnnn nnnnnnnnnn ncacccacgg cccttcccgc acgggtgcgg cggggatacg
    15961 cgctcggcgg cgtggcaacc gggtccttcg gaaccgtccc cggcctgatg ctcctgccct
    16021 acctcaccga acggctcggc gtgcccgcgg cgctagccgg cgtcatcgtc ttcgccccca
    16081 aagcctggga cgtcatcctc aacccgatcg ccggccggat cagcgatcac tcgacacatc
    16141 ccgacggccc gcgccgcccg ttcctgctgc gcgccggcct ggctctcgcg gtcgccttcg
    16201 ccctcctctt cgccggcccg gcctcaccgc aagccctcgc cgccggatgg gtggccgtgc
    16261 tcttcctcgc gtgcgcgacg gcatacgcct tcttccaggt gccctatgtc gcgatgcccg
    16321 ccgagctcac cgactccccc cgcgagcgga cccgcatcat gagcctgcgg gtggcggtcc
    16381 tcgcgctcgc gatcctcatc agcggcgggc tcgcgccgat ggtccgcacg atggtcggcc
    16441 cggagcgggg gtatgccgtg atgggcctgt tcgtcgccgt cctcatcgcg gtcggtgccc
    16501 tcggcgcgtg gtgggggacc cgtggcgcgc cagtgcgggc cgttgcggct gcgcccggcg
    16561 gtctgcgcag ccaattggcg ctcgtcgcag gcaatcccag cttccgggcg ctgatcctca
    16621 ccttcatcgt ccaggctctc gcgacggggg cgatgctcgc cggagtcgac tacgtcgcgc
    16681 gtcacgtgct gcgcgacccg ggcgccgggt cggtgctgtt tgtgtgcttc gtcgcccccg
    16741 ccatcgtctg cacgcccctc tgggagcggc tcgccgcccg cctcggcaag cgccgcgcgt
    16801 atgccgccgc gagcctcacc ctcgccgccg gggccgccgc tctggtcgcg gcccgggtca
    16861 tgccggccgc gttggtctat gtcgcggtgg ccgtcgtcgg ggtcgggtat gccggtgccc
    16921 agatgcttcc gctgtcgatg ctgcccgacg tggcagccgc cgatgccgcc gagtcggggg
    16981 agaaccgggt cggcgtcttc accggcgtgt ggaccgccgg cgagaccctc gggatggccc
    17041 tcggtccctt cgtcttcggc ctggtcctgg ccgccggagg gtatgtgtcc tccaccgact
    17101 cgactgcggc ccagccagat tcggcggtca tggcaatcgt gctcggcttc tcggtcctgc
    17161 cggcgctcct cacccttgcc tcgctcttct tcctccgggg ctactctccg ccgatgcccc
    17221 gcggcttcgc gacctacggc ccagaatccg aggtggatcg cccatgaccc tcactgccga
    17281 cgaggtccgc gcccgactgc tcaccctgcg tgagcatgac ctgccgaccc acgggggccg
    17341 aaccctcgct tatgtctacg actccggccg acccgacgtc gacgccgtcg cccgcgaggc
    17401 ggtcgcggcg ttcgcgggga cgaacggcct cgacccctcg gtgtttccct ctgtcgcaac
    17461 gatggagcgc ggcgtcgtcg acttccttgc cgggctactg tccgcacccg aggggtatgc
    17521 cggcaccgtc acctccggcg gcaccgagtc gtgtctgctc gccgtcctcg cggcccgcga
    17581 ctcccggccc gacgtcactc gcccgcagat cgtcgccccg accacggcgc atgcggcgtt
    17641 ccacaaggcg gcccatctct tcgggctcga gctgacgctc gtcgacgtcg acccggtcac
    17701 cttccgcgcc gatccggcag ccatggcggc ggccgtcacc gaccggaccg tcctcgtcgt
    17761 cgtcagcgcc ccgtcctatg cccacggcgt cgtcgacccc gtggggccga tcgccgcgat
    17821 ggcggccgag cgcggcatcc gctgccatgt cgacgcctgc atcggcggct tcgtgctgcc
    17881 cttcctcgcc gacgagtccg ctcgcggggt cgcgcagggc cgcccggcgt ggtcgttcga
    17941 ggtccccggc gtgacgagca tctcggccga cctgcacaag tacggctacg cgcccaaggg
    18001 cgtctcggcc ctcgtgcatc gctcaatggc gttgcggcac agccagttct gggccacggc
    18061 tgactggccc ggctacggcg tcgtcaactc caccctgcag tccaccaagt ccgccgggcc
    18121 tctcgccgct gcctggacca ccatcgaact cctcggcgag caggggtatg ccgccctcgc
    18181 ccggcaggcg cgcgacggtg ccctcgcgct cgcggcggag gtgggcggca taccggggct
    18241 gcgggtgctg gtgcccccgg actcctcgtt gctcgcggtc gtcaccgacg actcgtgcga
    18301 tccctacacg gtgtgcgacg agttgttgga gcgcgggtgg ttcgcccaac cgcaactgcc
    18361 cttccggggc gggcccgcca gcctgcatct gacgatctct gcagcgacca ccccgcaact
    18421 tccggagttc gttggcgcgc tggcggattc ggtggcagcg gcccaggtgg ccggtccggt
    18481 ggccgtcgac ccggcgatgg tcggtctgct ggccaccctc gatccggcga cgttcgacga
    18541 ggccaccctc ggcgcgctgc tggccgccgc cgggctgacc ggcgcgggtg gggcattgca
    18601 gttgcctcgc cggatggcgg cgatccaggc cctcctggct gcgtgccccc ctgcgctgcg
    18661 ccgcgtcctg ctcacggcgg tggtcgaacg gctctagagt tcatggtgag ttcaccttga
    18721 ggcgtcggcg cgcggggtcg tcgttgcgtt cagcggacaa cggcgcccga gcctgcgccg
    18781 ggtcctcgtc accagcacag gctcagaggc gagcatgatg aacagcgact acgagttgga
    18841 cacgacccgc cgcgacgcgc cgctgcgcat ctcggcccgc gggcgcgcgg tgctggccaa
    18901 ccccatgacc aaccgcggga cggccttcac cgtccaggag cggcgctacc tcggcctggt
    18961 cggcctcatg cccagtgagg tgacccccat cggcaaccag gtgcgccggg tctaccagca
    19021 gtaccactcg gcccggaccc cgttcgccaa gaacaactac ctcgccagcc tccgcgaccg
    19081 caacgaggtg ctcttctacc ggctgctcgc cgaccacatc gaaga
//
LOCUS       DAORPW010000034        18908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_512_length_18908_cov_423.485334, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000034 DAORPW010000000
VERSION     DAORPW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 18908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18908
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_512_length_18908_cov_423.485334"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..2269
                     /locus_tag="PLB86_04670"
     CDS             29..2269
                     /locus_tag="PLB86_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC64461.1"
                     /translation="MTEALLERRLSLIVEHFPIAVLLETVDRKVSQTNQAFCDMFGIP
                     APPAALVGADCEAAAVQLAPLWGDLDGFLARVHELLAAGKPVVGDRIELTDGRVLERD
                     FLMVPVDETRGEAAWIYRDVTVSDTARRVAQSEANARSELLATISHDVRTPVVGIVGL
                     VDILLQQPLDHRTRELVESVHSSAAAMTTMLDDLLDLSRADAGRLELRIEDTSICELV
                     EDVAGMVGPVAQAKSLPLIAGVKAAVPDIIRTDPGRLRQVLLNLTSNAVKFSPTGAVT
                     ILADREGSDLVVRVSDTGPGMTPEAITRAFEQYVQGGAEVNRAYGGAGLGLAIANKLT
                     AALGGTITVHSRIGLGTTFAVRIPGAVVGPELSTTMTGIRAHLTGHHRAVPVVAAALE
                     RAGIEVRDQADDEGVNLEVVVASSLAHAQRIPAASRRQLILVPAALASCPPLAGTALA
                     LPWTRERLRAALRDEWVADDVRERAGLLPLGTRVLLAEDEPSNRRIISEMLTRLRAEV
                     VAVGTGLEALEALAADRFDVVLMDLTMPVMGGVEAVENIRHRVPADRCPPILALTADA
                     RVDPSLRPDSGFCGHLSKPVTSAMLSRAVAGVLKASSPEPPPTLGERPAVDVTVLHAL
                     VEDVGDTQVVLDTIELYLEELPVRLSSMTAALTGERFDLLRDEAHALKSSSRMLGAGT
                     LADQCRDLEAVTAAHSRQDSASGGTVTPMVDALQFEAFRVAQWLADFRAASYPGLDVP
                     SASSGT"
     gene            complement(2227..3978)
                     /locus_tag="PLB86_04675"
     CDS             complement(2227..3978)
                     /locus_tag="PLB86_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC64462.1"
                     /translation="MSEQHPADAELRRQLARAEARLKRERAARRESEDIAEATLRRLY
                     LRQADVDLLSQVTSIANSAQEFESAFLDAMVLIRRASQWQVAHYFLPAKDDPTVMVSS
                     GVWSGEPDDPFLDELRSATSGMRFPPGSGIPGEAYLQGATWEEDIAASRNFPRQQWLT
                     GGSAFAFPILIGKEVVAVMEFLRSSPRRPQQGLLEIADVIGIQLGRVVERARARQSES
                     GHRTSLQDVVAQRTADLIAARNRAEAQSDARSTLFSTVSHDLQTPLHAALADIEAAAS
                     APDSGEHLARAVSHLIELGQRIRALVELAAPTDANADHPRVVDLSRVLDDVIAAHDAA
                     RRHRDTSLDVAVVFAQSDEVLVDVERFRRIIYTLLAGRLIEQSAGPVRIRLEVRATWA
                     DLTLDEPDPGSEGTTLGLARQLAHAAGGEICVHALPDRRRRIEVRIPVAVQGRHRRGI
                     GRRILLVDDIAVTRQISAAMLTRMGAVVDTAENGQQALDVLREHEYALVLMDLRMPIM
                     GGLEASRLIRAGEAGGERCDVPVVALTAHSAPGDADRSLLAGMDAHLTKPFTYDELRA
                     TVLLYVPELAEGTSRPG"
     gene            complement(3975..4523)
                     /locus_tag="PLB86_04680"
     CDS             complement(3975..4523)
                     /locus_tag="PLB86_04680"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HRC64463.1"
                     /translation="MKGVIFNVIEETVRAEHGEDAWDELLDTAGLDGAYTSLGTYPDH
                     EMAVLVQTASAALGLSQEELLRHLGRVGFSRLAARNADLMIDFKDARSLLLGLNSVIH
                     PEVRKVYPGADVPVFDVVSGQGHRMSLSYRSNRGMCYLGEGLALGAADHFGETMVVTQ
                     PICQHRGGDHCELQFIWPEARR"
     gene            complement(5278..6123)
                     /locus_tag="PLB86_04685"
     CDS             complement(5278..6123)
                     /locus_tag="PLB86_04685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRC64464.1"
                     /translation="MKISVIVPTRNSGRTLAACLGSLRRQTHEDVEIIVVDNASTDDT
                     PPIALQLAHIIVDRGPERSAQRNRGAWLASGEIVVFIDSDMVLEPTVLADLAATFEAR
                     PEVGAVIIPQRSFGVGFLASCRVLEKSLHVGLDDVEAPRAFRTEVFDLVGRWDETLSA
                     AEDWDLADRTRAAGVEIARVSSWIWHDEGKISLRDQFAKKRYDGRWVAEYLSRKPETR
                     QHVSRPGLRSQLPALLASPVRTGGLVILKSVETAGLVAGMSKAQRAGLVSPRRVSRGP
                     AGLCV"
     gene            complement(6120..6314)
                     /locus_tag="PLB86_04690"
     CDS             complement(6120..6314)
                     /locus_tag="PLB86_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64465.1"
                     /translation="MTTLTLSPALAIAPMALAPSAFAALAAVLVCTLRYAVLLPAPRG
                     RDERRNCDPIAYGDIPEEIS"
     gene            complement(6803..7495)
                     /locus_tag="PLB86_04695"
     CDS             complement(6803..7495)
                     /locus_tag="PLB86_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC64466.1"
                     /translation="MVAPVALLIEDSKEYALIGAKLLQREGFNVLVAHDGETGLGLAR
                     ARKPELVLLDIGLPGVDGFEVCRRLRDFSDAYLIMVTGRTDEVDRVIGLTMGADDYVT
                     KPFSARELAARIAAMRRRPRVVPDPELWDFGPLRIDVAARETTLDGELLDLTKVEFDL
                     LAKLASEPRRAFTRSQLLDAVWGVDWFSDDHVVDVHVGNLRKKLGESGSRPRFVHTVR
                     GVGFRFEPNSKR"
     assembly_gap    7538..7637
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7673..8737
                     /locus_tag="PLB86_04700"
     CDS             7673..8737
                     /locus_tag="PLB86_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRC64467.1"
                     /translation="MRVANARAALAALDSTARQLPNPKLLRRPSLRREAQSTSALEGT
                     YEPLQAVLTADDEGPTSLTMREVLNFVTMADVAFDWAAERRRLSASMLSELQRTLVRG
                     TPAEGPNSGFIRKIQVVIGQRRTASVGELPVRAARFVPSPPGADLEASVRDLVTWINE
                     DHRATIDPVVGAALAHYQFETLHPFHDGNGRIGRLMIVIQLYAAGVLGEPTITVSPWF
                     EARRTEYYDRLFAVSTQGEWDNWVSFFAQGLEESAVRTRQQMLDLVAVQSNLKEVVRA
                     SSLRADSAHKLVDYAVANTVFTVRQVERDLVLSYGRANGLVRDLVQLGILSESGDAYR
                     RRFFAPTVLDVLLSAEPGRG"
     gene            complement(8952..10544)
                     /locus_tag="PLB86_04705"
     CDS             complement(8952..10544)
                     /locus_tag="PLB86_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRC64468.1"
                     /translation="MTATAGVVSAGRAVLGIELGSTRIKAVLTDPSGQRLASGVHSWE
                     SRLEDGHWTYSQEHIEGGLVACVADLRRDLHEVHGISLSTVAALGVSAMMHGYIALDE
                     DDQWLVPFRTWRDTTTAQAAQELGCALGVNIPQRWSVAHYYQAHLDGESHLARVVRLT
                     TLAGWVHLRLTGEHILGVGDASGMFPIDTATGTYDQWMLGVVDDLMERHGTTGPSRPL
                     EHLLPRVLRAGRPAGTLTPAGARLLDPSGGFDPGALVCPPEGDAGTGMVATNSVRPRT
                     ANVSVGTSVFAMTVLDDVWRSADPNVDVVCTPEGAQVAMVHANNGAHELDAWAGVFAD
                     FARKLSLDVTRDDVFRVLLDAADEGANNAGGTVVHNFIAGEPVVGVEAGRPLMARGQS
                     DRLTLADLVRANVYTVFASLAIGMRSLRIAGVTTDNVVAHGGVFRTKGVAQQALAAAL
                     DSPVSVLRGAGEGGAWGMALLAALLLHPDLDLPDFLTQVVFASTAAETVVPNPEDVAG
                     FAEYLSRFEGLLPLQAMAAQLP"
     gene            10655..11674
                     /locus_tag="PLB86_04710"
     CDS             10655..11674
                     /locus_tag="PLB86_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRC64469.1"
                     /translation="MASAHPKTPVMADVALLAGVSHQTVSRVLNGHPHIRQSTRERVE
                     RAIAQLSYQPNTAARALVRGRSGLIGIVTGSGAFYGPRSAHQGVSAAARKAGYSVTSV
                     ELDDITEATLAAAVEDLSRLGVEGLIVVAGHDAAVDVARKRAGALPIVVVEGDLTRAA
                     MSAGVDQVAGAVAATEHLIGLGHRRIAHISGPLHWCEARARLEGWQLAMGRSGLPAVH
                     LLRGDWSPESGYTAGQQIAADAEITAVFASNDQMALGALRALREAGRSVPGDVSLVGF
                     DDIPEAAYLFPPLTTVHQDFAAVGHQAIAVLAAALSGAELPTPGLLSAPLIERASTAP
                     PRKDR"
     gene            11676..12353
                     /locus_tag="PLB86_04715"
     CDS             11676..12353
                     /locus_tag="PLB86_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HRC64470.1"
                     /translation="MGVAPELQQVVDMLRDQLSELHAELVCNHLVVWTAGNIAARVPG
                     HDLMVIKPSGVPYDELTPDAMVVCDLNGKVVEGERAPSSDSAAAAYIFRTMPHVGGVV
                     HTHSTYATAWAARGEAIPCVLTMMADEFGGEIPIGPFALIGDDSIGRGVVETLRASRS
                     TAVLMRQHGVFSVGRDARAAVKSAVMCEDVARTVHIARQLGQPIALQQNDIDSLYDRY
                     QNVYGQR"
     gene            12395..13915
                     /gene="araA"
                     /locus_tag="PLB86_04720"
     CDS             12395..13915
                     /gene="araA"
                     /locus_tag="PLB86_04720"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HRC64471.1"
                     /translation="MSTPLASRPLAVHFLTGSQEMYGEETLRQVAEQSRRVADELGAH
                     GLGVELQWQPVLTDSTSVHRAVLAANADEQCAGVITWMHTFSPAKMWIAGLDALRKPL
                     LHLHTQADARLPWGSIDMDFMNLNQAAHGDRELGHVVGRLGLARAIVSGHVSNPIVRQ
                     RVSEWASAARGAHELRTMRVARFGDNMREVAVTDGDKVEAQIRFGVSVNSYGVTELVN
                     LVAAAPEAALNELVTEYLDTYAVAPDLRPGAARHTSLRYGAAIEWGLRTFLADGGFTA
                     FTTNFEDLGGLRQLPGLAVQRLMADGYGFGGEGDWKTSVLLRTLKAMGAGRTGGTSFM
                     EDYTYDLSTPGEERILGAHMLEVCPSIAGTTPTLEIHPLSIGGKEDPVRLRFTAAPAP
                     GVVVGLSDLGDRFRLTLNEIEVVDPTEPLPRLPVASATWRPEPDFATSTESWILAGAP
                     HHTVLSTAVGTPELRHLAEMLRTELVLIDRHASVEGIRDRLRWNAAYHRLNNHLCV"
     gene            13927..15951
                     /locus_tag="PLB86_04725"
     CDS             13927..15951
                     /locus_tag="PLB86_04725"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HRC64472.1"
                     /translation="MTNSASKATPTSASALSVRASAAHRPVSPLLFGLFYEDINLSSD
                     GGLNANLVNNHSFEGVYLDPKHKELRALAMRTPTTKHLDRARHWETSGPAAKLSVLGT
                     DAVASHGHYGRLTGGSGATLTNHGYPGDAGPSVAVRAGVNLRFTALARGPQGGSRCHV
                     VLLAGDGNLVAEADFTVPAGDQWTQVVAELSPTRTVRASLQLVLPDGGTLDLDEVRLI
                     PEDHWGAGDPRWSQGLFRRDLVEALRDLAPRFMRFPGGCIVEGLTLENAYDWKASVGP
                     LAERRPNYNLWGMHRPDGDYSQSLQIGFYEYFLLCEDLGMEPMPVVSAGLACQFRSRE
                     VTPVDGADFSKVVQDTLDLIDWATGDPATNAWAAKRAAGGHPEPFRLNLLGIGNENHG
                     PVYLERFERIRAAVDAHHPGLTIIMSSGTAPAGKAFDQSWAHARTCGDAKTVVDEHFY
                     KSPAWFIDQVNRYDDYPRGGAQVFMGEYAAHVPTLPIPRRLRLPANTIKSAIAEAAFL
                     TGVERNADVVAMTSYAPLLNHLESELWQHNLIDFDGFTVTPTANAAVQKLFATTLGER
                     IVDLDGELPAGVFASASRSDDRLCVHLVNTTTIPQDVTLTVDLPLHGAGSATVLAAPH
                     GIQPSRRMGSRERAKLLEDHLALAVHDGAVSLTLRPQSVSALEFGLASVS"
     gene            16144..17028
                     /locus_tag="PLB86_04730"
     CDS             16144..17028
                     /locus_tag="PLB86_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC64473.1"
                     /translation="MGTAAGRQPSSEDERLSTPGADGVSREALLYRPRGGTSRGGLLW
                     IHGGGRVSGIPQFDDIQCRRLADDAGVTVLSVNYRLAPEHPFPAALDDCATALSWFTA
                     WCAANDIPGPLAIGGASAGGGLAAELAQRATDEGIELAHQVLVYPMLDDRSLGRGATN
                     RGRLVWTPTSNRFAWSAYLGHPAGASEQRPYAVAARREDLRGLPSAWIGVGDLDLFHD
                     EDVHYARRLRDAGVRVHLRVEPGMYHGADAVPGVKDSDRIRAFHADMSSSLRHALDLV
                     ESSHEPRDRSRDEATDGA"
     gene            complement(17089..18342)
                     /locus_tag="PLB86_04735"
     CDS             complement(17089..18342)
                     /locus_tag="PLB86_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HRC64474.1"
                     /translation="MIEYRPRIADGELAERMESAGAVLIEGPKSCGKTATASRRASTV
                     FRMDTDDSARDLVDTAPELLLGAAQPVLFDEWQVAPKLWNLVRRQVDDLGGEPGRFLL
                     TGSATPRDDATRHSGAGRYSILRMRPMSLFETGISTGAVSLAGLFDGDFTPSMAPNVT
                     VPNLVDCIVTGGWPALLDVPVRVAQRWVVDYLSSLVEVDLPQMGVRRDPATLRRFLAS
                     LARGTGTELSTQAIATDVAGPDRTLDRDTAAGYLDILARLMVTEDVPAWAPHMRSATP
                     LRKSAARFMVDPSLAVAALGVGPEQLLRDLNATGFLFESLVLRDLRVYSQPLRGRLAR
                     WRDNNNHEVDIIITLDDGRWGALEVKMNPAAADGAAASLLRFKDKVDLDKVGEPAFLG
                     VVTTRTAAVRRQDGVHILPIAALGP"
     gene            complement(18612..18684)
                     /locus_tag="PLB86_04740"
     tRNA            complement(18612..18684)
                     /locus_tag="PLB86_04740"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18649..18651),aa:Ala,seq:tgc)
BASE COUNT         3090 a         6475 c         6263 g         2980 t
ORIGIN      
        1 gctcggtcag gatggtgttg tgccgaacat gacggaagca ctgctagaac gacgtctctc
       61 gctgatcgtt gagcacttcc cgatcgccgt gctcctcgag accgtggacc ggaaggtgtc
      121 acagaccaac caggcgttct gtgacatgtt cggaatcccg gccccgcccg ccgccctcgt
      181 cggtgccgac tgtgaggctg ccgccgtcca gcttgctcca ctctggggcg acctcgatgg
      241 cttcctggcc cgtgtccacg aacttctcgc tgccggcaag cccgtcgtcg gggaccggat
      301 cgagttgacc gacgggcgag tgctcgaacg cgacttcctc atggtccccg tcgacgagac
      361 tcgcggcgag gccgcgtgga tctatcgcga tgtcaccgtg tcggacaccg ctcggcgcgt
      421 ggcgcagtcg gaggccaacg cacgcagcga gctcctggcc accatcagcc acgacgtccg
      481 cactcccgtc gtcgggatcg tcggcctggt cgacatcctc ctgcagcaac cgctggacca
      541 ccggacccgg gagttggtcg agagcgtgca cagctcggcc gcggccatga ccaccatgct
      601 tgatgacctg ctggacctct cccgcgccga cgccggtcgg ctggaactgc gcatcgaaga
      661 cacgagcatc tgcgagttgg tcgaggacgt cgccgggatg gtcggccccg tggcgcaggc
      721 caagtccctc ccgctcatcg ccggcgtcaa ggccgccgtc ccggacatca tccgcaccga
      781 tcccgggcgg ctgcgccagg tgctgctcaa cctgacctcc aacgccgtga agttcagccc
      841 gaccggggcg gtgacgatcc tcgctgaccg ggaggggtcc gacctcgtcg tccgggtgtc
      901 cgacaccggg ccgggcatga cccccgaggc catcacccgc gccttcgagc agtatgtcca
      961 gggcggcgct gaggtcaacc gcgcctacgg gggtgctggg ctcgggctgg ccatcgccaa
     1021 caagctgacg gcggcgttgg gtggcaccat caccgtccac agccgcatcg ggctggggac
     1081 caccttcgcc gtccggatcc ccggtgccgt cgtcggcccg gagctgagca ccaccatgac
     1141 cggcatacga gcgcatctca ccgggcacca ccgtgccgtc cctgtggtgg cggcagcact
     1201 ggaacgcgcg ggcatcgagg tgcgagacca ggccgacgac gagggggtca acctcgaagt
     1261 ggtcgtcgcc agcagcctgg cgcacgccca gcggattccg gcggcgagcc ggcgtcagct
     1321 catccttgtg cccgctgccc tggccagttg tcccccgctg gctggcaccg cgctcgccct
     1381 gccctggacc agggagcgat tgcgcgccgc cctccgagac gaatgggtgg cggacgacgt
     1441 gcgtgagcgt gctgggctgc tgcccctcgg aacccgggtc ctgctcgccg aggacgaacc
     1501 gagcaaccgc cggatcatct ccgagatgct cacccggctt cgggctgagg tcgttgccgt
     1561 gggcacgggg ctcgaagcgc tggaagccct ggcggccgac cgcttcgacg tcgtcctcat
     1621 ggacctgacg atgcccgtca tggggggcgt cgaggccgtc gagaacatcc ggcatcgggt
     1681 gccagccgac cgatgcccgc cgatcctcgc gctcaccgca gatgctcggg tggaccccag
     1741 cctgcggccg gacagcggat tctgcggaca cctgtcgaaa cccgtcacca gcgccatgct
     1801 cagtcgcgcc gtcgccgggg tgctgaaggc ctcctcgccg gagccaccgc ccaccttggg
     1861 cgagcggcct gcggtcgacg tcaccgtgct gcatgcgctc gtcgaggatg tcggtgacac
     1921 ccaggtcgtc ctcgacacca tcgagctcta cctcgaagag cttcccgtgc ggttgagctc
     1981 gatgacggct gcgctcaccg gcgagcgctt cgacctgctg cgcgacgagg cgcatgcgct
     2041 caagtcctcg tcacggatgc tcggtgccgg gacgttggcc gaccagtgtc gcgaccttga
     2101 ggcggtcacg gccgctcact cccgacagga cagcgccagc ggcggaacgg tgacccctat
     2161 ggtcgacgcc ctgcagttcg aggcgttccg ggtcgcgcaa tggttggcgg acttccgggc
     2221 cgcgagttac ccaggcctcg acgtgccttc ggcgagctcc ggcacataaa gcaggacggt
     2281 ggcgcgcagt tcgtcgtaag tgaacggctt ggtgaggtgg gcatccatcc cggcgaggag
     2341 gctgcggtcc gcgtcacccg gggccgagtg agccgtcaag gccaccacgg gcacgtcgca
     2401 gcgttcgccc cctgcctcgc cggcgcggat caggcgcgag gcctcgagcc cgcccatgat
     2461 cggcatacga aggtccatca gaacgagggc gtactcgtgc tcccggagca cgtcgagcgc
     2521 ttgttgcccg ttctcggccg tgtcgaccac ggcgcccatc cgggtgagca ttgcggcgct
     2581 gatctgacgg gtcacggcga tgtcgtccac gagaaggatc cgtcggccga tgccgcggcg
     2641 gtgccggccc tggacggcca cggggatccg cacctcgatc cggcggcgcc ggtcgggcag
     2701 ggcgtgcacg cagatctcgc ccccggccgc atgcgccaac tgacgggcga ggcccagggt
     2761 tgttccctcg ctgcccgggt cgggctcatc gagggtgagg tcggcccagg tggcccgcac
     2821 ctcgagtcgg atgcgcaccg gtccggcgga ctgttcgatc aatcgcccgg ccagcagggt
     2881 gtagatgatg cgacggaacc gttcgacatc gacgagcacc tcgtccgact gcgcgaagac
     2941 gacggccacg tccaaggagg tgtcgcgatg gcgcctggcc gcgtcgtgag cggcgatgac
     3001 gtcgtcgagc actcgtgaga ggtccaccac gcgagggtgg tcggcgttcg cgtcggtggg
     3061 agcggcgagc tcaaccaatg cgcgaatgcg ttgcccgagc tcgatgaggt gactcaccgc
     3121 ccgagccagg tgctcgccgg aatccggtgc ggacgccgcc gcctcgatgt cggcgagcgc
     3181 agcgtgtagc ggggtctgca ggtcgtggct gacggtggag aagagggttg aacgggcatc
     3241 tgactgtgcc tccgcgcggt tacgggccgc gatgaggtcg gccgtccgct gcgccacgac
     3301 atcctgcagg gaggtccggt gtcccgactc gctctggcgg gcgcgcgcgc gctcgacgac
     3361 tcggccgagc tggatcccga tgacgtcggc gatctcgagc aggccttgct ggggtcggcg
     3421 cggactggag cgcaggaact ccatgactgc cacgacctcc ttgccgatga ggatcgggaa
     3481 ggcgaaggcg gagccgccag tgagccactg ctgccgaggg aagttacgcg aggcggcgat
     3541 gtcttcctcc caggtagcgc cctggaggta ggcctcaccc gggatccccg agcccggagg
     3601 gaagcgcatg ccgctcgttg ccgaccgtag ctcgtcgagg aacggatcat cgggctcccc
     3661 actccagacc ccggaactca ccatgacggt cgggtcgtcc ttggccggca ggaagtagtg
     3721 ggcgacctgc cactggctcg cgcgccgaat gaggaccatg gcgtcgagga acgccgactc
     3781 gaactcctgc gcgctgttgg cgatggacgt cacctggctg agcagatcga cgtcggcctg
     3841 gcgcaggtaa agccggcgaa gggtggcctc ggcgatgtcc tcggactcgc gccgggcggc
     3901 ccgttcgcgc ttcagccggg cttcggcccg ggccagttgt cggcgaagct cggcatcggc
     3961 gggatgttgt tcactcaccg ccgggcctcg ggccagatga actgcagctc acaatggtcg
     4021 ccgccgcggt gctgacagat cggctgggtc acgaccatcg tctccccgaa gtggtcagcc
     4081 gcaccgagag cgaggccctc accgagatag cacatgcccc ggtttgagcg gtacgacagg
     4141 ctcatccggt gcccctgacc ggagacgacg tcgaacaccg ggacatccgc tccgggatag
     4201 accttgcgga cctcgggatg aatgacggag ttgaggccga gcagcagcga ccgtgcgtcc
     4261 ttgaagtcga tcatgaggtc cgcattacgc gcggccagcc gactgaaccc cactcggccg
     4321 aggtgccgca gcagctcctc ctggctcagg ccgagtgccg cgctcgccgt ctggaccagc
     4381 accgccatct cgtggtcggg gtaggtcccc aaggaggtgt aggcgccgtc cagcccggcg
     4441 gtgtccagaa gttcatccca ggcatcctcg ccatgttcgg cgcgcaccgt ctcctcgatg
     4501 acgttgaaga tgacaccctt cattgccaca ccctctcatt cctcgagccg tggcggcggc
     4561 cgtcgaaaca ctccacgccg gccgaggtct gccggctgcg tcaggcccgc gtctccttag
     4621 gtttgtggac aaaactcaag gactgctcaa ggggttctca ggacatcccc acgcatgggc
     4681 ctcgggccct tgacggccct taccgtggag ttatgcccac ccccacgctg ccggtcacgg
     4741 ctgctgaact tctggctcgg ccccccttgc ggggtcgttt gccggtgcga ggccgggtaa
     4801 gggaggcccg ggaggcctgg gggggcgccg ccatcggcga gcgcctggcc gcaatggacg
     4861 agcggcgcga gtgcctgggg gcgcccctcg tccgagacgt cgcacgggtc ctggaaggcg
     4921 cggaccgtac gaccgcgccg gtgggcggga cccggcgacg tctgcttctc gccaccgtcg
     4981 ctcccgccgc cgttgcgcga gcggctcgat agcgccgggt tcgctacgcg cgcagccttt
     5041 ttcggcatgg cgcatcgcaa cctacccggt gtcgccttag cccgccgggt gctgcgcgtc
     5101 gggggcgtgg agtcggtcac agccacgcct gtcgcagcac ccggtgccgc ggtggtcatc
     5161 gaccacgctt ccggtatgcc gctcgcggcc tccccgacac gcggaaggcg gtctcgctga
     5221 tcgttcagcc catcgccgtc ttcgccgaga actcgccgaa gacgtcgacg atgcgggtca
     5281 tacgcacagc ccggccggcc cacggctgac ccgccgcggg ctgaccaggc ctgcccgctg
     5341 cgccttgctc atgcccgcca ccaggcccgc agtctccacg gatttgagga tgaccaggcc
     5401 gcccgtgcgc accggcgagg ccagcagggc gggaagttgc gagcggaggc cggggcggct
     5461 cacgtgctgg cgagtctcgg gcttccggct gaggtactcg gccacccatc gaccgtcgta
     5521 tcgcttctta gcgaactggt ctcgtagcga gatcttgccc tcgtcgtgcc agatccagga
     5581 ggagacccgc gcgatctcga cgccggcggc tcgggtgcgg tccgcgaggt cccagtcctc
     5641 ggccgccgac agcgtctcgt cccagcggcc gacgaggtcg aacacctcgg tgcggaacgc
     5701 tcgcggagcc tcgacatcgt ccaacccgac gtgcaggctc ttctccagga cccggcaaga
     5761 ggccaggaag cccacaccga aggaacgctg cgggatgatg actgcaccca cctcgggacg
     5821 ggcttcgaag gtcgcggcga ggtcggccag gacggtcggt tcgaggacca tgtcggagtc
     5881 gatgaacacg acgatctcac cgctggcgag ccaggcaccc cggttgcgtt gcgcgctgcg
     5941 ctccggcccg cggtcgacga tgatgtgagc cagctgcagg gcgattggtg gggtgtcgtc
     6001 ggtcgaggcg ttgtcgacca cgatgatctc gacgtcctcg tgggtctgtc tgcggaggga
     6061 tcccagacat gcggcgagcg tcctcccgga gttgcgggtg gggacgatga cgctgatctt
     6121 catgagatct cctcagggat gtcgccgtag gcgatcgggt cgcagttgcg acgttcatcg
     6181 cggccgcggg gagccggcag cagcacggca taccggaggg tgcacaccag gacggcagcg
     6241 agcgcagcga acgcgctcgg cgccaacgcc atcggtgcga tggcgagggc gggtgagagc
     6301 gtcaacgtgg tcacttgacg aatgtcgcaa cgtcagatca agcgacgctc aagcccgtcg
     6361 caacgttgca gcaggcttgg ttgggcgggc tcggtgggcg gcggggaagc ggaggtcggc
     6421 caacgactgg accgagattg ccaccacgac cggcgcggcg agcgccgcca agcgaccggt
     6481 cgtttggggc cgcgcaccgc tcccggctcg ggggcgccag ggagcggtgt gcggcatgaa
     6541 catcagcgtg cccggctgat ctcaagaagg actcaagaga ggttccgggc ttgccggtca
     6601 tccgaccgcg gagtgacctt ggcgacgtgc tcccgcgccg gatcgatccc gtgaacgacc
     6661 acggaggagt cgttcgagat ggtgtcgacc aagacgcgga tgatcctcga ctccgagtcg
     6721 cagacgacca ttccggaggc acctcaaggg aggttcaagc gcgaggcaac gcgcggttca
     6781 gtccttcgcc ttttcggcgg gcttagcgtt tgctgttcgg ctcgaagcgg aacccgacgc
     6841 cgcgcaccgt gtgcacgaag cgtggtctgc tccctgattc gccgagcttc ttgcgcaggt
     6901 tgccgacgtg gacgtccacg acgtgatcgt cgctgaacca gtcgacgccc cacaccgcgt
     6961 cgagcagctg tgagcgggtg aaggcccgac gcggctcgga ggccagcttg gccaggaggt
     7021 cgaactcgac cttcgtcagg tcgaggagct cgccgtcgag ggtggtctcg cgagcggcga
     7081 cgtcgatgcg cagcgggccg aagtcccaga gctccgggtc ggggacgacc cgggggcggc
     7141 ggcgcatggc ggcgatccgc gccgccagct cgcgcgccga gaacggcttg gtgacgtaat
     7201 cgtcggcgcc catcgtcagg ccgatgaccc ggtcgacctc gtcggtgcgc ccggtgacca
     7261 tgatgaggta ggcatcggag aagtcgcgta ggcggcggca tacctcaaag ccgtcgactc
     7321 cgggcagacc gatgtcgagg agcacgagct cgggtttgcg cgcgcgggcc aggcccagcc
     7381 ccgtctcacc gtcgtgagcg accaggacgt tgaaaccctc gcgctgcagg agcttggcgc
     7441 cgatgagcgc gtactccttg ctgtcctcaa tgaggagcgc aaccggtgcg accatgcggg
     7501 acattgtcgc aggagcgccc ccgctcggcc cgttggtnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnngcg ccgacccgcc ggaccttgcg gccaagacct atctgcgcgt
     7681 cgcgaacgcc cgtgcagcgc tggcagccct agacagcact gcgcgacagc tccccaaccc
     7741 caagctgctt cgacgccctt cgctgcgtcg ggaggcacag agcacctcgg ctcttgaggg
     7801 gacctacgag ccacttcaag ccgttctgac cgccgacgac gagggcccca ccagcctcac
     7861 gatgcgcgag gtcctcaact ttgtgaccat ggctgacgtt gcgttcgact gggcagcgga
     7921 gcgccgccgg ctctctgctt ccatgttgtc ggagctccag cgcacgctcg tccgcggcac
     7981 tccagcggag ggccccaact ccggtttcat ccgcaagatt caggtggtga tcggacagcg
     8041 tcggacagct tccgtgggtg aacttcccgt cagggcggcg aggttcgtgc cctcgccacc
     8101 tggggccgac cttgaggcga gcgtccgcga cctcgtcacg tggatcaacg aggaccaccg
     8161 cgccacgatc gacccagtgg tgggggccgc cctcgcccac taccagttcg agaccctcca
     8221 ccccttccac gacggcaacg gccggatcgg acggctgatg atcgtcatcc agctgtatgc
     8281 cgccggcgtc ctcggggagc ccactatcac ggtctcaccg tggttcgagg ctcgtcgaac
     8341 cgagtactac gaccggctct tcgccgttag tacgcagggc gagtgggaca actgggtctc
     8401 cttcttcgcc cagggtctcg aggagtcggc agtgcggact cgacagcaga tgcttgacct
     8461 cgttgcggtc cagtcgaacc tcaaggaggt cgtcagggca tcgagccttc gcgcagacag
     8521 cgcgcacaag ctcgtcgact atgccgtggc aaacacggtc ttcaccgtgc gtcaagtgga
     8581 gcgtgacctg gttctctcat atggccgagc gaacggcctt gtcagagacc tggtccagtt
     8641 gggcatcctc tctgagtctg gcgacgccta tcgccggagg ttcttcgcgc cgacggtctt
     8701 ggacgtcctc ctgtccgctg agccaggtcg gggctaaacc ccagggatgc gaaagggccc
     8761 gaccaaaggc ggtcgggccc ttcttgtgtc aatcagtcag tcagtggtgg agccaagggg
     8821 acgccccacg gcgcccgccc gctccttcgt cgcggccagc cgccgcgtgg gcccagcaca
     8881 gcacgatcgc gggctagctg tgccagaact ggctctgttt cggggtacgc cgctgtcctg
     8941 caacctgacc gctacggcaa ctgcgcagcc attgcttgga ggggaaggag cccctcgaag
     9001 cggctgaggt actcggcgaa cccggcgaca tcctcagggt tcgggacgac cgtctcggct
     9061 gcggtcgatg caaagacaac ctgcgtcaag aagtcgggca gatcgaggtc tgggtgcagc
     9121 aagagggcgg cgaggagggc catgccccag gcccctcctt ctccggcccc gcgcaacacg
     9181 ctcaccggac tgtccagagc ggctgccaac gcctgctggg ccacgccctt ggtgcggaag
     9241 acgccgccgt gcgcgacgac gttgtcggtg gtgactcccg cgattcggag ggatcgcatg
     9301 ccgatggcga gcgaggcaaa gaccgtgtag acgttggctc ggacgagatc cgccaaggtc
     9361 aaccgatcgc tttgaccgcg ggccatgagt gggcggccgg cctccactcc cacaaccggt
     9421 tcgccggcga tgaagttgtg gaccaccgtt ccaccggcgt tgttggcgcc ctcgtcggct
     9481 gcgtcgagga ggacgcggaa gacgtcatcg cgagtcacgt cgagagacag cttgcgcgcg
     9541 aagtcggcga atacaccggc ccaggcatcg agttcgtgcg cgccattgtt ggcatgaacc
     9601 atggcgactt gtgcgccctc gggagtgcac acgacgtcga cgttcgggtc tgcgctgcgc
     9661 cacacgtcgt cgaggacggt catggcgaag acgctggtcc cgacgctcac attggccgtg
     9721 cgcggccgca cactgttcgt cgcgaccatc cccgtgccgg catccccctc gggagggcac
     9781 accagcgcgc cggggtcgaa gcctccggaa gggtcgagca gccgagctcc cgcaggtgtg
     9841 agcgtcccag ctggtcgtcc cgcgcgcagg actcggggca ggaggtgctc cagcgggcga
     9901 gaaggacctg tggtgccgtg gcgctccatc aggtcatcga ccacgcccag catccactgg
     9961 tcgtaggtgc cggtggccgt gtcgatcggg aacatgcccg aggcatcgcc aactccgagg
    10021 atgtgctcgc cggtcaggcg gaggtgaacc caaccggcca gggtggtcag gcgcaccact
    10081 cgcgcgaggt ggctttcacc gtcgagatgg gcctggtagt agtgggccac ggaccaccgt
    10141 tgggggatgt tgacgcccaa ggcgcaaccg agctcctgag cagcttgagc agtcgtggta
    10201 tcccgccagg tccggaatgg gacgagccat tggtcgtcct cgtccagagc aatgtaaccg
    10261 tgcatcatgg ccgacacccc gagagcggcc accgtgctca gcgagatgcc atgcacctcg
    10321 tggaggtcgc gccgcagatc ggccacacac gcaacgagcc ccccttcgat gtgctcctgg
    10381 gagtaggtcc agtggccgtc ctcgaggcga ctctcccaag agtgcacccc cgaggcgagg
    10441 cgctggcccg acgggtcggt gagcaccgcc ttgatccggg tcgaccccag ttcgatgccc
    10501 agcacagcgc gcccagccga gacgaccccc gccgtcgcgg tcatgacgcg cgcccacgtg
    10561 tgagcgtgca catgcgatcg agatccctga ccatcacagt tgacatggag gaatgttaac
    10621 gctaacaatt tggggggaca aggaggaccg cccaatggcc agcgcacatc ccaagacgcc
    10681 agtgatggca gacgtcgcgc tgctcgcagg ggtgtcccac cagaccgttt ccagggtgct
    10741 caatgggcac ccgcacatcc gtcagagcac ccgtgaaagg gtcgaacgag ccattgccca
    10801 gctgagttac caaccgaaca cggcagcgcg cgcgctcgtg cgagggcggt cggggctgat
    10861 cggcatagtc accggatcag gggccttcta cggaccgcga agcgctcacc aaggagtctc
    10921 ggcggcagcc cggaaagccg gctactcggt gaccagcgtc gagctcgacg acatcacgga
    10981 agccacgctc gccgccgctg tggaggatct ttctcgcctc ggcgtcgaag ggctgatcgt
    11041 cgtggcgggg cacgacgctg ctgtggatgt cgcccgcaag cgagcaggtg cccttcccat
    11101 cgttgttgtc gaaggtgacc tgacgcgggc ggcgatgagt gccggcgtgg atcaggtcgc
    11161 cggtgccgtg gccgcgaccg agcacctgat cggcctgggc caccgtcgaa tcgcgcacat
    11221 ttccggtccg ctccactggt gcgaggcccg ggcccggctg gaggggtggc agttggccat
    11281 ggggcgcagc ggcctgcccg cggtccacct ccttcggggc gactggagcc ccgaatccgg
    11341 ctacaccgct ggacagcaga tcgcagccga tgccgagatc acggcggtct tcgccagcaa
    11401 cgaccagatg gcccttggcg cactgcgagc cctccgggag gccggccgca gcgtccccgg
    11461 cgatgtctcc ctcgtcggct tcgacgacat ccccgaggct gcctacctgt tcccaccact
    11521 cacgaccgtg caccaagact ttgctgccgt cgggcaccag gccatcgcgg tcctggctgc
    11581 ggcgttgagc ggagccgagt tgcccacgcc cgggttgctc tccgcaccac tgatcgagcg
    11641 ggccagcacc gcaccgccgc gaaaggatcg ttgagatggg cgttgccccc gaactccagc
    11701 aggtcgtaga catgctccgc gatcagctca gcgagctgca cgccgagctc gtgtgcaacc
    11761 acctcgtagt gtggaccgcc ggcaacattg ccgcccgcgt ccccgggcac gacctcatgg
    11821 tcatcaaacc ctccggcgtg ccctacgacg aactcacgcc cgacgccatg gtcgtctgtg
    11881 atctaaacgg gaaggtcgtc gagggagaac gggccccgtc atcggactcg gccgctgcgg
    11941 catacatctt ccgaacgatg ccgcacgtcg ggggcgtcgt gcacactcac tcgacctatg
    12001 ccacggcgtg ggctgcgcgc ggcgaggcga tcccgtgtgt cctcacgatg atggccgacg
    12061 agttcggggg cgagatcccg atcgggccct tcgccctcat tggtgacgac tccatcggtc
    12121 gaggcgtggt cgagaccttg cgcgcgtccc ggtccacggc cgtgctcatg cgacagcacg
    12181 gcgtgttcag cgtcggccgc gacgctcgcg cagccgttaa gtccgcggtc atgtgcgagg
    12241 acgtcgctcg aaccgtccac atcgcgcgac agctcgggca accgattgcc ttgcagcaga
    12301 acgacattga ctcgctctac gaccgctacc aaaacgtcta cgggcagcgt tgaagcccgg
    12361 ccgcaacacc cagaaacacg ccgaggagac cagaatgtcc acgccccttg ccagccgccc
    12421 gttggcggtg cacttcctca ctggcagtca ggagatgtat ggcgaggaga ccctgcgcca
    12481 ggtcgccgaa cagtcgcgcc gtgtcgccga cgaactcgga gcgcatggcc tcggggtcga
    12541 actgcaatgg cagccggtgc tgacggactc gacctcagtg caccgcgccg tcctcgcggc
    12601 gaacgctgac gagcagtgcg caggcgtcat cacctggatg cacacgttct cgcccgcgaa
    12661 gatgtggatc gccggactcg acgcgctgcg caagccgctc ctgcacctgc acacccaggc
    12721 cgacgcgcgc ctgccatggg ggtccatcga catggacttc atgaacctca accaggccgc
    12781 acacggtgac cgagaactcg gccacgtcgt cggccgactc ggtctcgcgc gagcgatcgt
    12841 ctccggtcac gtgtccaatc cgattgtgcg acaacgggtt tccgagtggg ccagcgcagc
    12901 gcgcggtgct catgaactgc ggaccatgcg cgtcgcgagg ttcggcgaca acatgcgtga
    12961 ggtcgcggtc accgacggcg acaaggtcga ggcacagatc cgcttcggcg tctccgtcaa
    13021 cagctacggc gtcaccgaac tggtcaacct tgtcgccgcc gctccggagg cggcgctcaa
    13081 cgagctcgtc actgaatacc tcgacacgta tgccgtggca cccgacctgc ggccgggggc
    13141 cgcacggcat acgtccttgc ggtatggcgc cgcgatcgag tgggggctgc gcaccttcct
    13201 cgcggacggc gggttcacgg ccttcaccac caacttcgag gacctcgggg gactcaggca
    13261 gctcccgggc ctggcggtgc agcgattgat ggccgacggc tatggcttcg ggggcgaggg
    13321 ggactggaag acgtcggtcc tgctacgcac gctcaaggca atgggcgcgg gtcgaaccgg
    13381 tggcacctcg ttcatggagg actacaccta cgacctcagc actccggggg aggagcggat
    13441 ccttggcgcg cacatgctcg aggtctgccc gagcattgcc ggcaccacac cgacgctgga
    13501 gatccaccct ctgtcgatcg gcggcaagga ggacccagtc cggcttcgct tcaccgcggc
    13561 tccggctccg ggtgtcgtgg tcggcctgag tgacttgggg gaccggttca ggctcaccct
    13621 caacgagatc gaggtcgttg atcccaccga acctttgccg cgtctgcccg tcgcgtccgc
    13681 cacgtggcgt cccgaacctg acttcgcgac ctcgacggag tcctggattc ttgccggcgc
    13741 cccacaccac accgtgctga gcaccgcagt tggcacaccg gaactgcggc acctggcgga
    13801 gatgctgcgc accgaactgg tcctgatcga ccgtcatgcc tcggtcgagg gcatccggga
    13861 tcggctgcgc tggaacgccg cataccaccg cctgaacaac cacctctgtg tctgaggagc
    13921 aacccagtga ccaacagcgc cagcaaagcc acgcccacct ctgcatccgc cctcagcgtc
    13981 cgcgcctcgg cagcccaccg gccggtcagt cccctgctct tcggcctctt ctatgaggac
    14041 atcaacctct ccagcgacgg aggactcaac gccaacctcg tcaacaatca cagcttcgaa
    14101 ggggtttatc tcgaccccaa gcacaaggaa ttgcgcgctc tcgcgatgag gacccccacg
    14161 accaagcatc tcgatcgtgc tcggcactgg gagacctccg gacccgcggc caagctgagc
    14221 gtgctcggca ccgatgcggt ggcctcccac ggacactacg gacgcctcac cggcggatct
    14281 ggagccacgc tgaccaacca cggatacccc ggcgacgcgg ggccttccgt ggccgtgcgg
    14341 gcgggggtga acctgcggtt cacggccctc gcgcggggcc cgcagggcgg ttcacgatgc
    14401 cacgtggtct tgctggccgg ggacggcaac cttgtggctg aggctgattt cacggtgcca
    14461 gccggtgacc agtggaccca ggtcgttgcc gaactgtcgc ccacccgaac cgtgcgtgcc
    14521 tccctgcagc tcgtcctgcc cgatgggggc acactcgacc tcgacgaggt gcgcctgatc
    14581 cctgaggacc actggggtgc cggtgacccc cgctggagcc agggactgtt ccggcgcgat
    14641 ctggtcgagg cgctgcggga cctcgctccg cggttcatgc gcttccccgg cggctgcatc
    14701 gtcgaaggcc tcacgcttga gaacgcctac gactggaagg cctcggtcgg tccactggcc
    14761 gagcgtcgac ccaactacaa cctgtggggc atgcaccgcc ccgacggtga ctacagccag
    14821 tccctgcaga tcggcttcta cgaatacttc ctcctctgcg aggacctcgg gatggaacca
    14881 atgccggtgg tgtcggcggg actggcctgc cagttccgct cccgagaagt cacgccggtg
    14941 gacggcgcgg atttctcgaa ggtcgtccaa gacaccctgg acctcatcga ctgggccacc
    15001 ggtgatccgg cgaccaatgc gtgggcagcg aagcgggccg cagggggaca ccccgaacct
    15061 ttccggctca acctcttggg gatcggcaac gagaaccacg ggccggtcta cctggaaagg
    15121 ttcgaacgca tccgggccgc cgtcgacgcc caccaccccg gcctgaccat catcatgtcc
    15181 agcggtaccg cccccgccgg caaagccttc gaccagagct gggcacatgc gcggacctgc
    15241 ggcgacgcga aaaccgttgt cgatgagcac ttctataaaa gccccgcctg gttcatcgac
    15301 caagtcaacc gctacgacga ttacccgcgc ggcggtgctc aggtcttcat gggggagtat
    15361 gccgcacacg tacccacgct ccccatcccc cgccgcctcc gactccccgc caacacgatc
    15421 aagagcgcca tcgcggaggc agcgttcctc accggtgtcg agcgcaacgc cgatgtcgtc
    15481 gcgatgacct cgtatgcgcc gctgctcaac catctggagt ccgagctctg gcagcacaac
    15541 ctcatcgact tcgacgggtt cacggtcact cccaccgcca atgcagccgt ccagaagctg
    15601 ttcgcgacca ccctcggcga gcgcatcgtc gatctcgacg gcgagttgcc cgcgggtgtc
    15661 ttcgcgtccg ccagtcgttc cgatgaccga ctttgcgtcc atctcgtcaa cacgacgacg
    15721 atcccgcagg acgtgaccct caccgtcgac ctgccgctgc atggagcggg atcggccact
    15781 gtcctcgcag caccacacgg catacagcca tcccggcgca tgggctctcg cgagcgggca
    15841 aagctcctcg aggaccacct ggccctcgcc gtccacgatg gcgcggtcag cctcaccctc
    15901 cgcccccaga gtgtcagcgc acttgagttc ggactggctt cggtcagttg acctggacga
    15961 cctaacacgg aggtcaccta cccgatgcga acacctcaga cccctgccat cgcgctggcc
    16021 gcggccacgg ccgccgtagc ctggcgcgca cgacaccttc gccatgtcgc gcgtgagttc
    16081 cgcagccctg tcgtcatgct ggctctgcca gccacccgag ggacccttcc cttagtgcgg
    16141 gcgatgggca ccgcagcggg gcgacaacca tcctccgaag acgaacggct gagcacccca
    16201 ggcgccgacg gtgtgtcccg tgaggcgttg ctctaccgac cccgtggcgg gaccagtcgc
    16261 ggtggcctgc tctggattca cggcggcggc cgagtctccg gcataccgca gttcgacgac
    16321 atccagtgcc gtcgactcgc ggacgacgcc ggggtgacgg tgctcagcgt gaactaccgg
    16381 ttggcgcccg agcatccgtt tcccgctgcc ctggacgact gtgcgaccgc cttgtcgtgg
    16441 ttcaccgctt ggtgcgccgc caacgacatc cccggccccc tggccatcgg cggagccagc
    16501 gcggggggcg gactggcggc ggagctggcg cagcgggcga ccgatgaagg catcgaactc
    16561 gcccatcagg tcctcgtcta cccgatgctc gacgaccgaa gccttgggcg tggagccacg
    16621 aaccgtggac ggctcgtctg gaccccgact tccaaccggt tcgcgtggtc ggcctacctc
    16681 gggcatccgg cgggggcgag cgaacaacgt ccgtatgccg tggccgcccg ccgggaggat
    16741 ctgcgcggtt tgccctcggc gtggatcgga gtgggggacc tcgatctgtt ccacgacgag
    16801 gatgtccact acgcgcggcg cctccgcgac gcgggagtcc gggtccatct gcgcgtcgag
    16861 cccggcatgt accacggcgc cgacgccgtg cccggagtca aggactccga tcggatccgc
    16921 gcttttcacg cagacatgtc aagttctctt cggcacgcgc ttgatctggt cgagtcgagc
    16981 cacgagcccc gtgacaggag ccgggatgag gcgaccgatg gggcctgacg gccgagcgcc
    17041 ctcaacggcc gagcgccctc aaccgccgag cggtgacgtg ctgaaccgtt aggggccgag
    17101 tgcggcgatg gggaggatgt ggacgccgtc ctggcgacgc accgctgccg tccgggtggt
    17161 gacgacaccg aggaacgctg gttcgcccac tttgtcgagg tcgaccttgt ccttgaacct
    17221 cagcagggag gctgctgcac cgtcggcagc tgcggggttc atcttcacct caagtgcgcc
    17281 ccaccgtccg tcgtcgaggg tgatgatgat gtcgacctcg tggttgttgt tgtctcgcca
    17341 gcgggccaag cggccgcgga gcggttgcga gtagacgcgc aggtcgcgca acaccaagga
    17401 ctcgaagagg aatccggtcg cgttcaggtc acggagcaac tgctccgggc cgacaccgag
    17461 tgcggcgacg gccagtgatg ggtcgaccat gaaacgcgcc gctgacttgc gcaacggggt
    17521 cgccgagcgc atgtgagggg cccaggcggg gacatcctcg gtcaccatga gacgggccag
    17581 aatgtccaga tagccagcgg ccgtgtcccg atccaacgtc cggtcgggtc cggcaacgtc
    17641 ggtcgcgatg gcctgggtgc tgagctcggt gccggtgcca cgggccaagg acgcgaggaa
    17701 gcggcggagc gtggcggggt ctcgtcggac ccccatctgg ggcaggtcca cctcaaccaa
    17761 ggaactcagg tagtccacca cccaccgctg ggccaccctc accggcacgt ccaagagagc
    17821 gggccatccg cccgtgacga tgcagtccac caggtttggc accgtcacgt taggggccat
    17881 cgacggagtg aagtccccgt caaagagacc tgccagcgag actgcacccg tcgagatgcc
    17941 agtctcgaac aagctcatcg gacgcatccg aaggatcgag taccgtcccg cgccactgtg
    18001 ccgagtcgcg tcatctcggg gggtggccga ccccgtcagg aggaaccgcc ccggttcacc
    18061 tccgaggtca tcgacctgtc gacgcacgag gttccagagc ttcggggcca cctgccactc
    18121 atcgaacagc accggctgag cggcacccag gagcagttcg ggagcggtgt ccaccagatc
    18181 acgggcgctg tcgtcggtgt ccatccggaa cacggtcgag gcacgacgcg acgccgtggc
    18241 cgtcttgccg cacgacttcg gaccctcgat caggacggct cccgcggact ccatgcgttc
    18301 agccagttcg ccatccgcaa tgcggggccg atactcgatc acggtggcct cctcggtcaa
    18361 tccgataccc actaacccgt gaatcctata ctcaatattc cgcaaatcct atacctacgg
    18421 ccacgactgc cccggccccg gccaacgtcg acgtgaacgc agctgctgcg gagcttcacg
    18481 ccgatggtgt ccatctggag cgctggttgg attcgacctc aatggtcagg atgccggagc
    18541 gtcctcggcc aacgttcggt caagcgcgag gtcaacctgg ccgtcgaaac tgccgagacc
    18601 tgcgactgaa gtggagaaga cgggactcga acccgcaacc cccgccttgc aaaagcggtg
    18661 cgctaccaat tgcgccactt cccctggtga acgacttcgc cgactaagga cgagccgctg
    18721 agagcgacga agctcccgga gggactccaa gaggataccc gacctcgaag gtggtctcgc
    18781 ccgtctctag cccgtcctgg gccaggcggt caagccgcca caactcgccg ctgcgtgggc
    18841 cccctgcttg cacagcaggt gcgctaccaa ttgcgccatg gccccgaaac agccgggacg
    18901 gtcagcgg
//
LOCUS       DAORPW010000035        18592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_531_length_18592_cov_764.950801, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000035 DAORPW010000000
VERSION     DAORPW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 18592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18592
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_531_length_18592_cov_764.950801"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLB86_04745"
     CDS             <1..483
                     /locus_tag="PLB86_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRC64475.1"
                     /translation="AGSPSPTTATPTPASPAASPTTAATAAAPLVAQLPGIPVVAGKT
                     QSAATRILKAAGYTVSVVREEAADGVTGTVLRTSPEAGSALAPGSRVTLVVLQVVAEE
                     EPAGSNCTPGYDPCLPPASDYDCAGGSGNGPKYVEGPVRVTGSDPYRLDADHDGIGCE
                     "
     gene            complement(441..1334)
                     /locus_tag="PLB86_04750"
     CDS             complement(441..1334)
                     /locus_tag="PLB86_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC64476.1"
                     /translation="MAATVIDAHVHLWDLARRPQRWIDPVTMSVIHRDFGLTDLHAAQ
                     AEAGVQTGILVQVLNDPAETDDYFWLARDPALLGVIAWVDLLDPHVDAALDALAAHPS
                     GARLLGVRHQALAEPDPSGWLTAAGDGLGLRALARRGLPLDLILRPEHLAVAREVSGR
                     HTDLVMVLNHAGKAPVAAGWDSPEARHWATLVADLARLPQVACKLSGMTTMADLATWT
                     VADLRPFVEHLLACFGPDRLLFGSDWPVSLRAGGYAATVTAARALVAELSDSEQRAVL
                     SGTAERVYHSHPMPSWSASSR"
     gene            1479..2363
                     /locus_tag="PLB86_04755"
     CDS             1479..2363
                     /locus_tag="PLB86_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRC64477.1"
                     /translation="MPMSDFETIAWTVDADGIATLTLNRPDRLNSFTVTMARELEHAF
                     RHEALDDDVRAVVVTGSGRAFCAGMDLSAEGNVFGLDESLLPTPEHFWEHYADEPLHD
                     GVRDTGGRVTLAIHDLPKPVIAAVNGPAIGIGTTMTLAMDVRLASTTARFGLVFGKLG
                     IVPEAASTWFLPRIVGLAQALEWMYAAPVLTADAALAGRLVRSVHEAEDLLPAAYELA
                     RSFVVGKSPVALALTRKLLYRNVGVEHPLQAHLADSLGMYYTSIGDGREGVAAFLEKR
                     APHFNGRASDLPRITPEQ"
     gene            complement(3007..4311)
                     /locus_tag="PLB86_04760"
     CDS             complement(3007..4311)
                     /locus_tag="PLB86_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRC64478.1"
                     /translation="MDQVTDPESGTARHTSAPGGETADGGSSRCPVVHFDHTSAVHSL
                     DPVASYRALRAQGPVVWSPEHGGYWVLTDYASVFAAARDDETFSSARSAHGGEGLNNV
                     IPKAPVHLHIPVELDPPEHRTYRKIINAVTAPAALDGLRPRIAYWTARFVDDVIEAGQ
                     ADLATVIGVPAAVTLDWLGMDASQWQRYSHTMHSVLANRPGTDAHAHALAEGIPWMSQ
                     QITDAIAARRAQPRDDIVSYLVGAQVDGAPMSDDAAYSIIELLISGGVGTTASLVSQT
                     LVHLDADRSQRAELIEHPERLERAIEEFLRAFSPTQALARTIVRDTEIAGCPMRAGDR
                     ALLAWSSANRDPQQFEAPDEVRIDRWPNRHTAFGLGVHRCAGAHIARAIAAELLGQIL
                     TRMPDYVVDRERLRAYPQQGVNAGYEHIPATFTPGARLLPVE"
     gene            4455..5492
                     /locus_tag="PLB86_04765"
     CDS             4455..5492
                     /locus_tag="PLB86_04765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC64479.1"
                     /translation="MEIGVILGDVHGSLSPRDHLDALLRQVEAAQRAGMTYLTIGHHY
                     VYGDYRWLQPIPTLARLAAELDPGVRLGTTVLQTPLVHPIALAEDLATLDILSRGNLV
                     IGLGAGYRESEFVALGVNFADRFAMLEESIELMTALWTQPRVTHRGRFWQVDDVEPHI
                     QPWQQPHPPLWIGAMTERGVKRSARLADGWPITPETKIPDMVRLLAIYEDERIRLGKP
                     LVRHPLRREIIPGRTTEAAFERFESMAKERLLAYARRQLATRSEDELSQEFRSVAAKE
                     TFIGTPDECIAQIRELAALVPIDPILVRAQWPRMSADQVVDYLEDLGRDIVPAVREIT
                     PVQQVIRPPVA"
     assembly_gap    5726..5825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5826..6097
                     /locus_tag="PLB86_04770"
     CDS             <5826..6097
                     /locus_tag="PLB86_04770"
                     /inference="COORDINATES: protein motif:HMM:NF022279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2568 domain-containing protein"
                     /protein_id="HRC64480.1"
                     /translation="GWLGWVLGLLVAVLVIVAWSVWMAPRASHRIAPVLRAIVAGFGC
                     LGVGSALLSQGPHWWGIALLMGAVVMVVAELKPDRGREPSDSGGR"
     gene            complement(6099..7253)
                     /locus_tag="PLB86_04775"
     CDS             complement(6099..7253)
                     /locus_tag="PLB86_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRC64481.1"
                     /translation="MTGPLHGVRVVELAGIGPGPFAAMLLADMGADVVRVDRVDPPSR
                     DYTPNAVIERGRRSIAVDLRRPEGAAIVRRLASGADVLVESNRPGVLERLGLGPDVLL
                     ADNPRLVIGRLTGYGQDGPLAGAVGHDLNYISLTGALHTIGRAGEPPPPPLNLVGDFG
                     GAGSALAFGVVCALWEARGSGAGQVVDANALESTAALMGLVHGLRAAGRWSLDRGANL
                     LDGGTPYYDTYTCADGRWVAVATVEEKFFLELLRGLGLDDDPDLVGAHKDRSRWPALR
                     AALMAVFASRTRDEWAVHFAPLDACVTPVLDLDEAAGHPHAVARGGYVATEGTAYPQP
                     AVSPRFSRSSTPLPGAPARPGEHTRAVLTEAGYAAAQVDELLATGVARQA"
     gene            complement(7250..8785)
                     /locus_tag="PLB86_04780"
     CDS             complement(7250..8785)
                     /locus_tag="PLB86_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC64482.1"
                     /translation="MTATGDGSGDLGGLLGYWAAATPSAPALTWVPFDGPPVAWTYAA
                     LRHDVSRLAAGLARRGVRAGDPVALVMPNCPDVVLVWAALLQLGAVAVCADPRSSAAE
                     LGYVLEHSRAVGAVVDPVVTARAREPLDKLPWVVESSGRPEVGRGTGLLMADPPAGWA
                     TVPAQAPASIQYTSGTTSRPKAVVWTQANCLWAGRVGAAHQGLTQADTNLVHLPLFHT
                     NALSYSLLSTLFAGGHVVLQPRFSASRFWPVAVAHGATWSALVSFCVRALQTREPLPG
                     HRFRGWGNSAALDPAPWSGGVAALGWFGMTETVSHPIIGSLDPPDPPGTMGRPAREYA
                     VAVLHPDGSPVGAGEVGDLRVRGIPGVSLFAEYLRDPEATAAAVDADGWLRTGDRVRL
                     NEGGTLSFVEREGDLLKVGGENVGAAEIERVLLQAPGVAEVAVVGAPHEMLGEVPVAF
                     VLPRAGASVTVAEVEARCVAGLSPMRRPREIRIVSELPRVTLDKVAKAVLRGILREEG
                     VRR"
     gene            complement(8782..9138)
                     /locus_tag="PLB86_04785"
     CDS             complement(8782..9138)
                     /locus_tag="PLB86_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64483.1"
                     /translation="MILREVAYARSGDKGDTSNICVFVHEPADYPMLVERLTAERVRQ
                     HFAGLVRGEVVRYELPRVQGLNFVLFQALDGGVSATLRIDPHGKSFQSLALAMDVGDG
                     WSARPLGEASGTVGGA"
     gene            complement(9135..10478)
                     /locus_tag="PLB86_04790"
     CDS             complement(9135..10478)
                     /locus_tag="PLB86_04790"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HRC64484.1"
                     /translation="MPRGAIQVGVSIGAGSAYANDRLAPAAAMADSGRVTYIGFDSLA
                     ERTMALAHQRRRRDPATGQDERIRELVPLLAGYLGRGHRVVGNFGAANPDAAVADLVD
                     SLRAQGQHGIPIGVIRGDAVLDLVRSLDCALPERGTTVAALGDRVVSANAYIGADPIV
                     TCLEAGAQIVVGGRIADPSLFVGPICHELGWALDDWDAVAHATMVGHLLECGVHSTGG
                     NFEDPPYRVVPDPHRLGFPLADVTRADAIITKLPGTGGAVDARTMKTQLYYEIHDPAA
                     YLTPDVTADFSDIEIDDLGGDRVRLWGARGRPRPETLKVLVGVDDGFRATGEISFGGP
                     GCVTRARRAEEVLRAYLQPYAASLTDVRVDLVGLTSLFGDRPTSPEPAEVRLRLAVHT
                     PSRAVADAAAHEVELLYFGPSGGGGVVTSVVPALGVTPAFVPRELVTLTTEVVRS"
     gene            complement(10484..10678)
                     /locus_tag="PLB86_04795"
     CDS             complement(10484..10678)
                     /locus_tag="PLB86_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRC64485.1"
                     /translation="MKVQVNEERCQGHGLCRLSAPNFFFARVEDGHAEVRSEDVPDEE
                     REAVLLAAESCPELAIDVSD"
     gene            10792..12033
                     /locus_tag="PLB86_04800"
     CDS             10792..12033
                     /locus_tag="PLB86_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRC64486.1"
                     /translation="MTETQRCPVVHFDHNSDEHSADPAGSYKRIRDEHPAAWSEAHGG
                     YWVLSGYRTVFDASRDDATFSSARNEHGGEGLSVVIPKTPMHFHIPIEVDPPDFRKWR
                     KLINPITAPAAVERMETMVRHYVTWFIDEIIEKGSCDMAEVIGVPSIVTVDWIGLDPA
                     DWKRYAFAHRMVLAAVPGSDDWKQAIEVEFPYINARTWEAIADRRINPTDDMISYFVQ
                     QEIDGRPITDDEVFSIVDLLLAGGVATTASLVSNTVVWLYQHQDVRQQLIDDPSLLDK
                     AIEEFLRFFSPTQALARTVTQDTEFHGCPMREGDRVLLSWASANRDPEQFEDPDDVNI
                     ERWPNRHTAFGIGVHRCAGSNLGRFMAKEMLSQILTRMGDYVIDLDSLQAYPHQGTNT
                     GWQRIPATYTPGPRILPVPAG"
     assembly_gap    12194..12293
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12294..13522
                     /locus_tag="PLB86_04805"
     CDS             <12294..13522
                     /locus_tag="PLB86_04805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRC64487.1"
                     /translation="VVGAGLAGLRAAEQLRAAGHAGSIEVIGGEPHLPYNRPPLTKQA
                     LVAGPDVMTLMFPRRPSIENVRWTLGRRAVSVDLDGSTLSLDDGSTRDFDGLVIATGV
                     RSRRLPIGEGGGPAPTLTLRTVDDALALHARLAPGARVVVIGAGFIGCEVAATVAARG
                     GSVHVLDPLPAPLAGVLGPAVGSAVRARLEAAGVAFSLGVGVAQVLATPTGGYAVHLT
                     DSADVITADVVVEAVGSVPETEWLAGQGLDLGNGIGCDGDLLVTRGGIPLRNVVALGD
                     VARFPHPWFGREIRVEHWAWPTDLARRAAASLVAGLGPTPAPDTPFTPLPSFWSDLPG
                     GRIQSFGLPPLGLGDVRLLEGEWEGDCAVGYHRDGRLVGLALVGLASRMIDYRTALLD
                     DLSTGTGAGADAGPTG"
     gene            13519..14751
                     /locus_tag="PLB86_04810"
     CDS             13519..14751
                     /locus_tag="PLB86_04810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC64488.1"
                     /translation="MNRAGPRADPDPARGGSARRAWALAALVTLFGLNLRATLGSIPP
                     LLGEIATDLGLGSSALGLLLSTCILAMGAFAPTGHLLATRVGTERALTLLLAILGVSE
                     VVRVAAYHPVVLYASVALAGATMGAASTLMPGFIAHHVPRTRGLTMGIYSTGLALGVA
                     LAAATAVPLERLLGGWRPALAVVGLLSLATAVLSWASEGTLVRSMPGHSVDPPRTRDP
                     LPWRSVPARWLTLLNALVMAIGFSGLAWVAPYYVDLGMSVQDAAGMVVLFQVVQLVAM
                     LALPGITDVTTDRRPLVGGMLLATLAGTVMLGVAPMAQPLLAVGLFGLGAGGMATLLL
                     VLSVDLTDSRSASASLNAMTMLVGYAVGAAGPFLLGAARDVTGSLGPGYLVVSGLVVV
                     SLVIVSVFRPGLKVARAD"
     gene            complement(14759..15331)
                     /locus_tag="PLB86_04815"
     CDS             complement(14759..15331)
                     /locus_tag="PLB86_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRC64489.1"
                     /translation="MAERSLDERIRRLEDRAAIQELGILYGFVMDERDGDGIREIFCE
                     DATLRSQDGVFKANGIEEIVETYLGRFAALGPTNHFSHGHVVILDDTDPDRATGLIAS
                     HAEVSRNGVAMQVALRYKDTYRRVDGRWRFADRLMSYMYYLPFTELSEGLGDRNSVRA
                     YGDHRPSDWPEVLYAETGNAWLAPYYTSRG"
     gene            complement(15381..15689)
                     /locus_tag="PLB86_04820"
     CDS             complement(15381..15689)
                     /locus_tag="PLB86_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64490.1"
                     /translation="MPKKRPHDRADLFMAPVLLDVDERISQLAQLDAQALADRVLVHV
                     NHETSDGAERRDALLATLTDGLELHGWKAKWHDRGLRLTHGEHSVVLGLGPELRAYLS
                     "
     gene            15782..17434
                     /locus_tag="PLB86_04825"
     CDS             15782..17434
                     /locus_tag="PLB86_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRC64491.1"
                     /translation="MPDDVLAHPESSGSATDVDVLVLGTGAGGLTAALAAATAGARVG
                     LYEKGERVGGTTALSSAVAWLPANRYAAQAGIADSREDALAYLSALSHGLIRPELAEA
                     FVDTVPELLDWLDEQTPLRMRLVAGFPDYHPEHPGGMPRGGRSLEPELFPFTEIPGWG
                     ERMVGVTRPMLVAETPIGGGSGLLPADVAAQRKAASLEGVGRGLVGALLKGCLDRGVE
                     PVTGARATRLVVTDGAVTGVEFDSGAERFVVTARSVILATGGFEHDLDLVRSFLRGPM
                     HLPAGVPTNTGDGLRMAMRVGAELGMMSEAWWVPVLALPQADPAATHRPVLLVNLERT
                     LPRSIMVNGQGRRFTNEAANYNALGGAFHHLDPTSFAYVNQPAWLIIDQGFVDRFGGF
                     GVPPGGPAQPWLIRADDLGALAEATGIPPTALSDTVVRWNEQAAALRDDDFHRGQSAY
                     DGWSGERRHYPGPAATLGPLDEPPFYAVEIVGSCLGTKGGPRTDVDGRVQHLDGGPIP
                     GLYAAGNAMAAPTGMVYGGAGGTLGPALVFGYRAGRSAAREC"
     gene            complement(17461..17766)
                     /locus_tag="PLB86_04830"
     CDS             complement(17461..17766)
                     /locus_tag="PLB86_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HRC64492.1"
                     /translation="MSHQPAELPVVDVHETGGCACGEHDEAVPELDARTIPHAIRHAA
                     IFGALDSVRPGHAMVLVAPHDPLPLLAQISARSGDAVEVSYLQRGPEAWRLRLHRVR"
     gene            complement(17810..18556)
                     /locus_tag="PLB86_04835"
     CDS             complement(17810..18556)
                     /locus_tag="PLB86_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC64493.1"
                     /translation="MLNTPYRSPVLGPAPTQEPTAVATPAQGEVLAALSADGPATAAE
                     LGARTGQHPNTVREHLDALVDLGLAARDRDAPAGRGRPAYRYAALPHPSEGPAYRALI
                     AALVEHFVDGSSRGALGHSPASTITERATLLGRGVPVPESVAELARASGEAGGAAKAR
                     RTVAQAMATVMAGQGFRTEELPRGRGLRLVNCPLVGVAVRHGEVVCGFHQGMLQAVVE
                     RSGGDPDSVHLEPFAEPGACLVRIGPATSS"
BASE COUNT         2654 a         6550 c         6349 g         2839 t
ORIGIN      
        1 gcggggtcgc cgtcgccgac gaccgccacg ccgacaccgg cctccccagc cgcgtcaccc
       61 acgacggccg ccaccgcggc ggcgcccctg gtggcccaac ttcccgggat cccggtcgtg
      121 gccggcaaga cccagtcagc cgcgactcgc atcctcaagg ccgccggcta cacggtcagc
      181 gtcgtcaggg aagaggccgc tgacggcgtc actggcaccg tcctgcggac gtcacccgag
      241 gcgggctcgg cgctcgcgcc gggaagccgc gtcaccctcg tggtcctgca ggtggtcgcg
      301 gaggaggagc ctgcggggtc caactgcact ccgggctacg atccgtgcct tccgccggcg
      361 agtgactacg actgcgcagg gggcagtggc aacggcccga agtatgtcga gggtcccgtg
      421 cgcgtgacgg gctctgaccc ctatcggctc gacgccgacc acgacggcat cgggtgcgag
      481 tgatagaccc gctcggcagt gccggacagg acagcgcgct gctcactgtc ggaaagctcg
      541 gccacgaggg cgcgcgcggc ggtgacggtc gcggcatacc ctcctgcccg caggctcacc
      601 ggccaatcgg agccgaagag taagcgatcc gggccgaagc acgcgagcag gtgctcgacg
      661 aacggccgca ggtccgcgac ggtccaggtg gcgaggtcag ccatcgtcgt catccccgac
      721 agtttgcacg cgacctgcgg caaccgcgcg aggtcggcca ccagggttgc ccagtgccgc
      781 gcctcggggg agtcccaccc ggccgcgacg ggcgccttcc ccgcgtggtt gaggaccatg
      841 acgaggtcgg tatgccgccc gctcacctcc cgcgcgacgg ccaggtgctc ggggcgcaag
      901 atgagatcga gcggcagccc ccgtcgggcc agggcgcgca gcccgagtcc atcgccggcg
      961 gcagtcaacc agccggacgg gtcgggctcg gccagcgcct ggtgacgcac cccgagcagg
     1021 cgggcgccgg aggggtgggc ggcgagggcg tcgagggccg cgtcgacgtg cgggtcgagc
     1081 aggtcgaccc acgcgatcac cccgagcagc gctgggtcac gggccagcca gaagtagtcg
     1141 tcggtctcgg ccggatcgtt gagcacctgg acgaggatgc cggtctggac gcccgcctcg
     1201 gcctgggccg cgtgcaagtc ggtcagcccg aagtcgcggt ggatgactga catggtgacc
     1261 gggtcgatcc accgctgagg tcggcgggcg aggtcccaga ggtggacatg cgcgtcgatc
     1321 accgtcgctg ccacgctcgc ctcctgtgat gcgatcccac atctaaccat atgatatgca
     1381 tagataatgt cttcccccct accgattcga ggtattccgc catcgtctgg cgacggcgga
     1441 atacctcgac ccggcgcagc gccagccacg gaagtgagat gccgatgtca gacttcgaga
     1501 ccatcgcctg gaccgtcgat gcggacggca tcgccaccct caccctcaac cggcccgacc
     1561 ggctcaactc cttcaccgtg acgatggctc gcgagcttga gcacgccttc cgccacgaag
     1621 cgctcgatga cgacgtccgc gcggttgtcg tcaccggcag cggcagagcg ttctgtgcgg
     1681 gcatggacct ctcggctgag ggcaacgtct tcggcctcga cgagtcgctg cttccgaccc
     1741 ccgagcactt ctgggagcac tacgccgacg agccgctgca tgacggcgtg cgcgacaccg
     1801 gcggccgggt caccctcgcg atccacgacc tccccaagcc ggtcatcgcc gcggtcaatg
     1861 gcccagcgat cggcatcggc acgacgatga ccctcgcgat ggacgtccgg ctggcctcga
     1921 cgacggcgcg gttcggcctg gtcttcggca aactcggcat cgtcccggag gccgcgtcca
     1981 cctggttcct cccgcggatc gtcgggctcg cacaggccct cgagtggatg tatgccgcgc
     2041 ccgtcctcac cgccgacgcg gccctcgcgg ggcggctggt tcgctcggtg cacgaggccg
     2101 aggacctgtt gcccgcggca tacgagttgg cgcggtcgtt cgtcgtcggg aagtctccgg
     2161 tcgcgctcgc cttgacccgg aaactgctct accgcaacgt cggcgttgag cacccgctgc
     2221 aggcccacct ggccgactcg ctcgggatgt attacacgtc gatcggcgac gggcgcgagg
     2281 gcgttgccgc gttcctggag aagcgggcgc cgcacttcaa cggccgcgcc tcggacctcc
     2341 ctcggatcac cccggagcaa tgatcaggac cggcgcttga cgcgctggtt ccggatgctg
     2401 cctcgcagat gcaggggcga ctgctggcgg cgaagcctcg cttgtgttga gttatcacga
     2461 ctacccttag acagttaggg cagtcccctg aaggattcct ccccatttct ttcggacctg
     2521 aatgcacgca gcccgccaga ggacatcatg aacctgacct cctaccagaa gacgcgagtg
     2581 ggtctggttg cctggggtgc ggctggcctg ttgctcactc tgggcgcggt gtccctgggg
     2641 catgtcgcct gggtcatttc catggcgttc ctgctgatct ccgcggtcgt ccgggcccct
     2701 ggtcgtccgt ccgccctgtt ctttggggct tccgttccac tggttctggt tgtcgtgggt
     2761 cacgccgcgg gccccggttg gtcgtgccac tccagtccca caggttctgg gtgcgatgag
     2821 ttgctcgacc ctcgacccta cttgatggcg gcgttggtgc tgcttgccac cggcactgca
     2881 ctcgaacttc tgttgcggcg agcgagttcg cgtggcacgg cgatccacag ttcttgacga
     2941 ttcctcgtca cctctgatcg ttaaggccac tgccgcagaa cgaaattcat ggttgaggtg
     3001 gcgttctcac tccaccggca gcagccgagc gcctggcgtg aatgtcgccg ggatgtgctc
     3061 gtagcccgcg ttgactccct gctgcggata ggcgcgcagc cgttcgcggt cgacgacgta
     3121 gtcgggcatc cgcgtgagga tctgccccaa caactcggcc gcgatggccc gcgcgatatg
     3181 cgctcccgcg cagcgatgca cccccagccc gaaggccgta tgccggttgg gccagcggtc
     3241 gatccgcacc tcgtcgggcg cctcgaactg ctgcggatca cggttggccg agctccacgc
     3301 gagcagcgcc cgatcgccgg cccgcatcgg gcaaccggca atctcggtgt ctcgcacaat
     3361 ggttcgtgcc aacgcctgcg tcggtgagaa cgcccgcagg aactcctcga tcgcccgctc
     3421 cagccgctcc gggtgctcga tgagctctgc gcgctgcgag cgatcggcgt caaggtgcac
     3481 gagtgtctgg ctcaccagcg aggccgtcgt ccccacgcct cccgagatga gcaattcgat
     3541 gatgctgtat gccgcgtcgt cgctcatcgg ggcaccgtcc acctgcgcgc cgacgaggta
     3601 ggacacgatg tcgtccctcg gctgcgccct ccgggccgcg atggcgtcgg tgatctgctg
     3661 gctcatccac gggatgccct cagcgagcgc gtgcgcatgc gcgtccgtgc ccggccggtt
     3721 ggcgagcacc gagtgcatgg tgtgcgaata ccgttgccat tggctcgcat ccatgccgag
     3781 ccagtcgagc gtcacggccg ccgggacccc gatgacagtc gcgagatccg cctgcccagc
     3841 ttcgatgacg tcgtcgacga accgcgctgt ccaataggcg atccgcggac gcagcccgtc
     3901 gagcgccgct ggcgcggtga ccgcgttgat gatcttgcga taggtgcggt gctccggcgg
     3961 gtcgagctcg accgggatgt gcaggtgcac cggcgccttg gggatgacgt tgttcagccc
     4021 ctcgccgccg tgcgcggacc gcgccgacga gaaggtctcg tcatcccgcg cggccgcgaa
     4081 caccgacgca tagtcggtca acacccagta gccgccgtgc tccgggctcc acaccaccgg
     4141 cccctgcgcc cgtagcgccc gataggacgc cactgggtcc agcgagtgca cggcgcttgt
     4201 gtggtcgaaa tgcaccacgg gacagcgact cgacccacca tcggcggtct cgccgccagg
     4261 cgcgctggta tgccgcgcag tcccggactc ggggtcggtc acctgatcca tggggcacct
     4321 tccacgtcgt cggcattgac tggctctgac ccttgctgcg ggcgctggcc cggctctcgg
     4381 acgccagccc tcccgcatcg cggattccat gcatatgata gccatcatct ggtgatcgcg
     4441 gacggagctg gcccatggag atcggcgtca tcctcggcga cgtgcacggc tcgctttcgc
     4501 cgcgggacca cctcgacgcg ctgctgcgcc aggtcgaggc cgcccagcgg gccgggatga
     4561 cctacctgac catcggccac cactacgtct acggcgacta ccgctggctg caacccattc
     4621 cgacgctcgc ccggctggcc gccgaattgg accctggggt tcggctcggg acgaccgtcc
     4681 tgcagacccc gctcgtccac ccgatcgccc tcgccgaaga cctggcgacg ctcgacatcc
     4741 tcagccgggg caatctcgtc atcggactcg gcgccggcta ccgggagtcc gagtttgtcg
     4801 ctctcggagt caatttcgcc gatcgctttg ccatgctgga agagtcgatc gagctgatga
     4861 ccgcgctgtg gacccagcct cgggtcaccc accggggccg cttctggcag gtcgacgacg
     4921 tcgagccgca catccagccc tggcaacagc cgcacccacc gttgtggatc ggcgccatga
     4981 ctgagcgggg cgtcaagcgg tcggcacgcc tcgccgacgg gtggcccatc acccccgaga
     5041 ccaagatccc cgacatggtg cggctcctcg cgatctacga ggacgagcgg atccgcctcg
     5101 gcaagcccct cgttcgccac ccgttgcgcc gcgagatcat ccccggccgc acgaccgagg
     5161 cggccttcga gcggttcgag tcgatggcca aggagcgcct gctcgcctat gcccggcgcc
     5221 aactcgccac ccgctcggag gacgagctct cccaggagtt ccgctctgtc gcagccaaag
     5281 agaccttcat cggcacgccc gacgaatgca tcgcccagat ccgtgagctc gccgccctcg
     5341 tccccatcga ccccatcctc gtgcgcgccc aatggccgcg gatgagcgcc gaccaggtcg
     5401 tcgactacct cgaggacctc ggccgggaca tcgtccccgc cgtccgcgag atcacgccgg
     5461 tccagcaggt catccgccca ccggtcgcct agggtcgcga atgtgagcgg gtatgtcgtc
     5521 ctgtctgcag tggtccgcgc cggggtggcc gtgccggccg aatcttcggg ggcctgatgc
     5581 cgctcgcggt cggcgtgctc ggggcgctcg cgtttgcgac ggagctgctg ctgtgggcag
     5641 gtgtggccgt ggcggtccag gctgcggtcg ggggttggct gggttgggtc cttggtctgt
     5701 tggtggccgt cctggtcatc gtcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnggggt tggctgggtt gggtccttgg tctgttggtg gccgtcctgg tcatcgtcgc
     5881 ctggtcggtg tggatggccc ctcgagccag ccatcggatc gctcccgtac tccgcgcgat
     5941 cgtggccgga ttcggctgtc ttggcgtcgg gtcagcactg ctctcgcaag gcccgcactg
     6001 gtgggggatc gctctcctca tgggtgccgt cgtcatggtg gtcgccgaac tcaaacctga
     6061 ccgcgggcgt gagccttccg actcgggcgg ccgctagctc acgcctgtcg ggccaccccg
     6121 gtggcgagca actcatcgac ctgcgcggcg gcatacccgg cctcggtgag gaccgcgcgg
     6181 gtgtgctcgc cgggacgggc gggcgcgccc gggagggggg tcgagctccg cgagaagcgt
     6241 ggggacaccg ccggctgcgg gtatgccgtg ccctccgtcg cgacgtagcc tccccgcgcg
     6301 accgcatggg gatgcccggc ggcctcgtcg agatccagca cgggggtcac gcacgcatcc
     6361 agcggggcga agtgcaccgc ccactcgtct cgggtccgac tggcgaacac cgccatcaac
     6421 gccgcccgca gggctggcca ccgtgaccga tccttgtgcg cgccaacgag atccgggtca
     6481 tcgtcaagcc ccaaccccct cagcaactca aggaagaact tctcctcgac cgtcgcgacc
     6541 gccacccaac ggccatccgc gcaggtgtag gtgtcgtaat agggcgtccc cccgtccagg
     6601 aggttggccc cgcgatccag gctccagcga ccggccgcgc gcagcccgtg taccaacccc
     6661 atcaaggccg ctgtcgactc cagcgcattg gcatcgacga cctgcccggc ccccgaccca
     6721 cgggcctccc acagtgcgca gacgaccccg aaagccagcg ccgacccagc cccaccaaag
     6781 tcgccgacca ggttgagcgg cgggggcggg ggttccccgg cccggccgat ggtgtgcagc
     6841 gcccccgtga gcgagatgta gttgaggtcg tggccgaccg ctccggcgag cggtccgtcc
     6901 tggccataac cggtgagccg cccgatgacc agtcgagggt tgtcggcgag caacacgtcc
     6961 ggccccagcc ccagccgctc cagcacccca ggccggttgc tctcgacgag cacgtctgcg
     7021 ccagaggcca atcggcgcac gatcgccgcg ccctcgggtc ggcgcaggtc gaccgcgatc
     7081 gagcggcgac cccgttcgat cacggcgttg ggtgtgtaat cccgcgatgg cgggtcgact
     7141 cgatcgaccc gcacgacatc ggcgcccatg tcggccagca gcatcgccgc gaacggcccc
     7201 ggcccgatcc cggcgagttc gacgacgcgc actccgtgca acggcccagt catcggcgca
     7261 ccccctcctc gcgcagtatg ccgcgcagca ccgcctttgc gaccttgtcg agcgtcacgc
     7321 gtggcaactc gctgacgatg cggatctcgc gtggtcgccg catggggctc agcccggcga
     7381 cacaacgggc ctccacctcg gcgacagtga cgctcgcccc ggctcggggc agcacgaacg
     7441 ccaccggcac ctcgccgagc atctcgtgcg gtgccccgac gaccgccacc tcagcgacgc
     7501 ccggcgcctg caacagcacc cgctcgatct ccgccgcgcc gacgttttcg ccgccgacct
     7561 tgagcagatc gccctcccgc tcaacgaaac tcagcgtccc gccctcgttc aaccgcaccc
     7621 gatcaccggt ccgcaaccag ccgtcggcat cgaccgccgc agcggtcgcc tccggatcgc
     7681 gcagatactc cgcgaacaac gacacgccag gtatgccgcg cacccgcagg tcgccgacct
     7741 cgcccgcccc cacgggggag ccgtcggggt gcaggacggc gacggcatac tcccgcgcgg
     7801 ggcgacccat cgtgcccggt gggtcgggcg ggtccagcga gccgatgatc ggatggctca
     7861 ccgtctcggt catcccgaac cagccgagcg ccgccacccc gcccgaccat ggcgccgggt
     7921 cgagcgcagc gctgttgccc caaccgcgga accggtggcc aggcaacggt tctcgggtct
     7981 gcagcgcacg cacgcagaaa gacacgaggg ccgaccaggt cgcgccgtgt gccacggcga
     8041 ccggccagaa gcgggaggcc gagaagcgtg gctgcagcac gacgtgaccg cccgcgaaga
     8101 gcgtcgagag cagcgagtag gacaacgcgt tggtgtggaa gagcggcagg tgcacgaggt
     8161 tggtgtcggc ctgggtcagc ccctgatggg cagcgccgac ccggcccgcc cacaggcagt
     8221 tggcctgggt ccagacgacg gccttggggc gcgaggtcgt ccccgaggtg tattggatcg
     8281 aggccggtgc ctgggccggc acagtcgccc accccgccgg agggtcggcc atgagcaacc
     8341 cggttccccg ccccacctcc gggcgcccac tgctctccac cacccagggc agtttgtcga
     8401 ggggctcccg cgcacgtgcc gtcacaacgg ggtcgacgac ggctccgaca gcgcgcgaat
     8461 gctccaagac atagcccaat tcggctgcgc tagagcgcgg gtcagcacag acggccaccg
     8521 cgccgagttg caacagcgcc gcccagacca ggacgacatc ggggcagttg ggcatgacga
     8581 gggccaccgg gtcgccggct cggacacccc ggcgagccag cccggcggcg agccgggaga
     8641 cgtcgtggcg gagggcggca tacgtccacg cgaccggagg accgtcgaag gggacccagg
     8701 tgagcgccgg agcggacggc gtggctgctg cccaatagcc gagcaacccg cccaggtcgc
     8761 cgcttccgtc gccagtggcg gtcatgcccc gccgacagtg cccgacgctt cgcccaaggg
     8821 ccgcgccgac cagccgtcac cgacgtccat cgccagcgcc aacgactgaa aagacttgcc
     8881 gtgcgggtcg attcgcaaag tcgccgagac cccgccgtcg agggcctgga acagcacgaa
     8941 gttgaggccc tgcactcgcg gcaactcata gcggacgacc tcgccgcgca ccaaccccgc
     9001 gaagtgttgc ctgacccgct cggcggtgag ccgctcgacg agcatcggat agtcggccgg
     9061 ctcgtggacg aacacgcaga tgttgctcgt gtcgcccttg tcgccggagc gggcataggc
     9121 cacttcgcgg aggatcacga gcgcaccacc tcggttgtga gagtgaccaa ttcgcgaggc
     9181 acgaaggcgg gcgtgacgcc gagcgcaggg accaccgagg tgacgacccc gccgccgccc
     9241 gatggcccga agtagagcaa ctccacctcg tgcgcggccg catcggcgac ggcccgggag
     9301 ggggtgtgaa ccgccaaacg cagtcgcacc tcggccggct cggggctcgt gggtcggtct
     9361 ccgaagagcg aagtgagccc cacgaggtcg acgcggacgt cggtgagcga cgcggcatac
     9421 ggctgcaggt aggcgcgcag gacctcctcg gcgcgccgcg cgcgggtcac gcacccgggc
     9481 ccgccgaacg agatctcgcc cgtcgcgcgg aagccgtcgt cgacgccgac gagcaccttg
     9541 agggtctcgg gccgtggacg tccgcgggct ccccatagcc gcactcggtc gccgccgagg
     9601 tcgtcgatct cgatgtcgga gaagtcggcg gtcacgtccg gcgtcaggta tgccgcgggg
     9661 tcgtgaatct cgtaatacag ctgggtcttc atcgtgcggg catcgaccgc accacccgtg
     9721 ccggggagtt tggtgatgat cgcgtcggcc cgagtgacgt cggccagcgg gaagccgagc
     9781 cgatgcgggt cggggacgac gcggtagggc gggtcctcga agttgccccc agtgctgtgc
     9841 actccacact ccagcaggtg gccgaccatc gtcgcgtgcg cgacggcgtc ccagtcgtcc
     9901 agcgcccacc cgagctcgtg gcagatcggc ccgacgaaca gcgacgggtc ggcgatccgc
     9961 ccgcccacga cgatctgcgc gccggcctcc aggcaggtga cgatcgggtc ggcgccgatg
    10021 taggcgttgg ccgagacgac ccggtcaccc agggcggcga ccgtcgtgcc ccgttctggg
    10081 agagcgcagt ccaaggaccg gacgaggtca aggacggcgt cgccgcggat gaccccaatg
    10141 ggtatgccgt gctgcccctg ggcgcgtagc gagtcgacga ggtcggccac ggccgcgtcg
    10201 gggttggcgg cgccgaagtt gccgacgacc cggtggccgc gtccgaggta acccgcgagc
    10261 agcgggacga gttcgcggat gcgttcgtcc tggccggtcg ccggatcgcg ccgccggcgc
    10321 tgatgggcca gcgccatcgt gcgctcggcg agcgagtcga acccgatgta ggtgacccgc
    10381 cccgagtcgg ccatcgcggc cgccggggcg agtcggtcgt tggcgtaggc cgaaccggcg
    10441 ccgatcgaca ccccaacctg gatggcgcct cgaggcattg cgctcagtcc gagacgtcga
    10501 tggcgagctc ggggcagctc tcggcggcca ggagcacggc ctcgcgctcc tcgtcaggca
    10561 catcctccga gcggacttcg gcgtggccgt cctcgacccg cgcgaagaag aagttcggcg
    10621 cgctcagccg gcacaggccg tgaccctggc agcgctcctc gttgacctgg accttcatgt
    10681 tgccctccac gtgtcgttga caggaccatt cagtataacc ataatcacat taaggcgtgg
    10741 agggaagaga gccacgcgaa cggcccatca gcagcctagg gaggcccaac catgaccgag
    10801 acccagcggt gcccagtcgt ccatttcgac cacaactccg acgaacactc ggccgacccg
    10861 gcgggctcct acaagcgcat ccgggacgag caccccgctg cgtggagcga ggctcacggc
    10921 ggctattggg tgctctccgg ctaccgcacg gtgttcgacg cctcccgaga cgacgcgacg
    10981 ttctcgtcgg cgcgcaacga gcacggcggt gaaggtctct cggtggtcat ccccaagacc
    11041 cccatgcact tccacatccc catcgaggtc gacccgcccg acttccgcaa gtggcgcaaa
    11101 ctcatcaacc cgatcaccgc tcccgctgcc gtcgagcgga tggagacgat ggtccgccac
    11161 tacgtcacgt ggttcatcga cgagatcatc gagaagggct cgtgcgacat ggccgaggtc
    11221 atcggcgtgc cctcgatcgt caccgtcgac tggatcggtc tggaccccgc cgactggaag
    11281 cgctacgcct tcgcccaccg catggtcctc gccgcggtgc cgggcagcga cgactggaag
    11341 caggccatcg aggtcgagtt cccctacatc aacgcccgca cgtgggaggc catcgccgac
    11401 cgccggatca accccaccga cgacatgatc agctacttcg tccagcagga gatcgacggg
    11461 cgtccgatca ccgacgacga ggtcttctcg atcgtcgacc tgctgctggc cggtggcgtc
    11521 gccacgaccg cgtcgctcgt ctccaacacg gtggtctggc tctaccagca ccaggatgtg
    11581 cgccagcaac tcatcgacga cccgagcctg ttggacaagg cgatcgagga gttcctgcgg
    11641 ttcttctccc cgacccaggc gttggcccgc accgtcaccc aggacaccga gttccacgga
    11701 tgcccgatgc gcgaaggtga ccgggtgctc ctgtcgtggg cctcggccaa ccgcgacccc
    11761 gagcagttcg aggaccccga cgacgtcaac atcgagcgct ggcccaaccg gcataccgcg
    11821 ttcgggatcg gcgtgcaccg ttgtgccggc tcgaacctcg gccggttcat ggccaaggag
    11881 atgctgtccc agatcctcac ccggatgggc gactacgtca tcgacctcga ctccctgcag
    11941 gcctacccgc accagggcac gaacaccgga tggcagcgga tcccggccac ctacacgccc
    12001 ggcccgcgca tccttcctgt tccggccggc tgagccccgc tgagcccggc tgagcccggc
    12061 tgagcccgga tcgtccccgt cacccgaccc gtccccacgt caccgcagag gctgatcacc
    12121 ccatgaatcc gcacgtgctc gtcgtgggtg ccggtctggc cggcctacgg gcggccgagc
    12181 agttgcgcgc cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcgtcgt
    12301 gggtgccggt ctggccggcc tacgggcggc cgagcagttg cgcgccgcag gtcacgcggg
    12361 gtcgatcgag gtcatcggcg gcgagccgca cctgccctac aaccggccgc cgctgaccaa
    12421 acaggccctc gtcgccggcc ccgatgtgat gaccctgatg ttcccgcgtc gaccgtcgat
    12481 cgagaacgtt cgctggaccc tcggtcggcg cgcggtctcg gtcgacctcg acgggtcgac
    12541 gctcagcctc gacgatgggt ccacccgtga cttcgacggg ctcgtcatcg ccacgggtgt
    12601 ccgctcgcgg cgactgccga tcggcgaggg cggcggccct gctccgacac tgaccctgcg
    12661 gaccgtcgac gatgcgctcg ccctgcacgc tcggctcgcc ccgggcgcgc gggtggtcgt
    12721 catcggcgcc ggcttcatcg gttgtgaggt ggccgcgaca gtggcagctc gcggagggtc
    12781 ggtccacgtc ctggaccccc tgccggcacc gttggcaggc gtccttgggc ccgcggtggg
    12841 ctccgcagtc cgcgcgcggc tggaagccgc gggggtggcc ttcagcctcg gcgtcggcgt
    12901 cgcccaggtg ctcgccaccc ccaccggtgg gtatgccgtg cacctcaccg acagcgcgga
    12961 cgtcatcacg gccgacgtcg tcgtcgaggc cgtcggctcg gtgcccgaaa ccgagtggct
    13021 cgccgggcag ggcctggacc tgggcaacgg aatcggctgc gacggcgacc tattggtgac
    13081 gcgaggcggc atacccctga ggaacgtcgt cgcgctcggt gacgtcgcgc gcttccccca
    13141 cccgtggttc ggtcgggaga tccgggtcga acactgggcc tggcccaccg acctggcccg
    13201 gcgagccgcc gccagcctgg tcgccgggct cgggccgacg cccgccccgg acaccccgtt
    13261 caccccgctg ccaagtttct ggagcgacct gcccggtggg cggatccagt ccttcggtct
    13321 gccgccgctc ggtctgggcg acgtgcggtt gctcgaaggt gagtgggagg gcgactgcgc
    13381 cgtgggctac caccgcgacg gacgtctggt cgggctggcc ctggtcgggc tggcctcccg
    13441 gatgatcgac taccgcaccg ccctgcttga cgacctgagc accggcacgg gcgctggcgc
    13501 cgacgccggg ccgaccgggt gaaccgcgcc gggccgcgag cggaccccga tccggctcgc
    13561 ggcggctcgg cgcgacgtgc ctgggcgttg gcggcgttgg tcaccctctt cgggctcaat
    13621 ctgcgggcca cgctgggatc gatccctccg ctgctcggtg agatcgccac ggacctcggg
    13681 ctgggttcca gcgccttggg attgctgctg tccacctgca tcctcgccat gggcgccttc
    13741 gcgccgaccg gccatctcct ggccacccgg gtcggcaccg aacgggcgct gaccctcctg
    13801 ctggcgatcc tcggggtgtc cgaggtggtg cgcgtcgcgg cataccaccc ggtcgtgctc
    13861 tatgcctcgg tggcgctggc gggcgcgacc atgggcgcgg cctccaccct catgcccggt
    13921 ttcatcgccc accacgtgcc acgaacccgc gggttgacga tggggatcta ctcgacgggg
    13981 ctggcgctcg gggtcgcgct ggcggctgcg accgctgtgc cgctcgagcg gctcctcggg
    14041 ggctggcgtc cggcgctcgc cgtggtcggc ctcctgtcgc tggccacggc ggtcctctcg
    14101 tgggcgtcgg aggggacgct ggtgcgaagc atgcctgggc acagcgtcga cccgccgcgc
    14161 acccgtgatc cgctgccctg gcgcagtgtt ccggctcgct ggctcaccct gctcaacgca
    14221 ctcgtgatgg ccattggctt cagcggattg gcttgggtgg caccgtatta cgtcgacctc
    14281 gggatgtcgg tccaggacgc ggccggcatg gtggtgctct ttcaggtcgt ccaactcgtg
    14341 gcgatgctgg ccctgccggg catcaccgat gtcacgaccg accgtcggcc gttggtgggc
    14401 ggaatgttgc tggcgaccct cgccggcacg gtcatgctcg gggtggcgcc gatggctcag
    14461 ccgctgctcg cagtcgggct cttcgggctg ggggcgggcg gcatggccac gctgctgttg
    14521 gtgttgagcg tcgacctcac cgactcgcgg tcggcgtcgg cgtccctcaa tgcgatgacg
    14581 atgctcgtcg gttatgccgt cggcgcggcc gggccgttcc tgctcggggc ggcgcgggac
    14641 gtgaccggca gcctggggcc gggctatctc gtcgtgtcag ggctggtcgt cgtcagcctg
    14701 gtgatcgtct cggtcttccg gcccggcctc aaggttgcgc gggccgacta ggggcgcgtc
    14761 agccgcggga ggtgtagtag ggcgcgagcc aggcgttgcc ggtctcggcg tagagcacct
    14821 ctggccagtc tgagggccgg tggtcgccat aggcgcggac gctgttgcgg tcgcccaggc
    14881 cttcggacaa ctcggtgaac ggcaggtagt acatgtagga catcagccgg tcagcgaagc
    14941 gccaacggcc gtcgacccgg cggtaggtgt ccttgtagcg caacgcgacc tgcatggcga
    15001 cgccgttgcg cgagacctcg gcgtggctgg cgatcagccc cgtggcgcgg tcggggtccg
    15061 tgtcgtcgag gatgacgacg tgcccgtggc tgaagtggtt ggtcgggccg agcgcggcga
    15121 accggccgag gtaggtctcg acgatctcct cgatcccgtt ggccttgaag accccgtcct
    15181 gggagcgcag ggtcgcgtcc tcgcagaaga tctcacggat gccgtccccg tcgcgttcgt
    15241 ccatgacgaa cccgtagagg atgcccaact cctggatcgc ggcccggtct tccaggcgac
    15301 ggatccgctc gtcgaggctg cgttcggcca tgaggactcc tttgaactcg gcagtgcagg
    15361 ggtcaaaagc agtgctgcgg tcaggagagg taggcgcgca actcgggccc gaggcccagc
    15421 acgaccgaat gctcaccgtg ggtgagtcgc aggccgcggt cgtgccattt ggccttccaa
    15481 ccgtgcaact cgaggccgtc ggtcagcgtg gccagcaagg cgtcccgccg ttcggctccg
    15541 tcgctcgtct cgtggttgac gtgcacgagg acccggtcgg cgagggcctg ggcatcgagc
    15601 tgggccagtt ggctgatccg ctcgtcgacg tcgagcagga ccggcgccat gaacagatca
    15661 gcccgatcgt gggggcgctt cttcggcatg cggtgctcct tcatgcggtt gtcacggcgc
    15721 atcgaagcgc agattcagta taaccatgat taccataggg ctggaagaag gagggcgcca
    15781 gatgcctgac gacgtgctcg ctcaccccga gtcttcgggt tcggctaccg acgtggatgt
    15841 cctcgttctc gggacggggg ccggcggcct caccgccgcg ttggcggccg ccacggcggg
    15901 cgcgcgggtc gggctctacg aaaagggcga gcgagtcggc ggcacgacgg ccctctccag
    15961 cgcggtcgcc tggctccccg ccaaccgcta tgccgcgcag gcgggcatcg cggactcccg
    16021 cgaggacgcc ctggcctatc tgtcggccct ctcccacggg ctcattcgac cggagttggc
    16081 cgaggccttc gtcgacacgg tgccggagtt gcttgactgg ctcgacgagc agaccccgct
    16141 gcggatgcgg ctcgtggccg gcttccccga ctatcacccc gagcaccccg ggggtatgcc
    16201 gcgtggcggc cgatcactcg agccggagtt gttccccttc accgagattc cggggtgggg
    16261 tgagcgaatg gtcggtgtga cccgaccgat gctcgtcgcc gagactccga tcgggggcgg
    16321 gtcggggttg cttcccgccg atgtcgcggc gcagcgcaag gccgcctcgc tggagggcgt
    16381 cgggcgcggg ctggtcggcg ccctgctcaa gggttgtctc gatcgtgggg ttgagccggt
    16441 caccggagcg cgggcgaccc gactggtggt gaccgacggc gccgtgaccg gcgtcgagtt
    16501 cgactccggt gcagagcgat tcgtcgtcac tgctcgctcc gtgatcctcg cgaccggcgg
    16561 cttcgagcac gacctcgacc tggtccgcag cttcctgcgc ggtcccatgc accttccggc
    16621 aggcgtgccg accaacaccg gtgacgggct gcggatggcc atgcgggtcg gcgccgagtt
    16681 ggggatgatg agtgaggcgt ggtgggtccc cgtgctcgcg ctcccgcagg ccgacccggc
    16741 cgcgacccac cggccggtgc tgctggtcaa cctcgagcgc accctgccgc gttcgatcat
    16801 ggtcaacggt cagggccggc gcttcaccaa cgaggcggcc aactacaacg ccctcggcgg
    16861 ggccttccac cacctcgatc ccacgagctt cgcctacgtc aaccagccgg cttggttgat
    16921 catcgaccaa ggtttcgtcg accggttcgg cggcttcggc gtgcctccgg gaggtccggc
    16981 gcagccgtgg ctcatccgcg ccgacgacct cggcgcgctc gccgaggcga ccggcatacc
    17041 tccaactgcc ttgtccgaca cggtggttcg gtggaatgag caggcggcgg cgctgcgcga
    17101 cgacgacttc caccgcggcc agagcgccta tgacgggtgg agtggcgaac ggcggcacta
    17161 cccggggccg gccgccacgt tggggccgct cgacgaaccg ccgttctacg cggtggagat
    17221 cgtcggctcg tgcctgggga ccaagggcgg cccgcgcacg gacgtggacg gccgcgtcca
    17281 acacctcgac ggtggcccga tccccggtct gtatgccgcg ggcaacgcca tggcggcgcc
    17341 gaccggcatg gtctacggcg gggcgggcgg aaccctcggc ccggcgctgg tcttcggcta
    17401 ccgtgcggga aggtcggcag ccagggagtg ctgaccgcgc caggcctcgg gatcctccgg
    17461 tcagcgcacc cggtgcaggc gcaggcgcca cgcctcgggg ccgcgctgca ggtagctcac
    17521 ctcgacagcg tcccccgaac gggcgctgat ctgggccagc agcggcagcg ggtcgtgcgg
    17581 ggcgacgagg accatcgcgt gccccggccg caccgagtcg agcgcgccga agatcgcggc
    17641 gtgccgaatg gcgtgcggga tggtgcgggc gtcgagttct ggcaccgcct cgtcgtgctc
    17701 accgcaggcg cagccgcccg tctcgtggac gtcgacgacg ggcaactcgg caggctggtg
    17761 cgacatgcgt atttctcctc aggtggggat ggacgggtgg tggatcggct caggacgagg
    17821 tcgctgggcc gatccggacg aggcacgcgc cgggttcggc gaacggctcg aggtgcactg
    17881 agtcggggtc gcccccagat cgttcgacga cggcctgcag catcccctgg tggaagccgc
    17941 agacaacctc gccatgtcga accgcgaccc cgaccagcgg gcaattgacc agacgcagcc
    18001 ctcggccgcg cggaagttct tcggtgcgaa agccctgtcc agccatcact gtggccatcg
    18061 cttgggccac cgtccggcgg gccttggcgg cgcccccggc ctcgccgctc gccctcgcaa
    18121 gctcggccac cgactcgggg acgggaacgc ctcgccccag cagggtggcg cgttcggtga
    18181 tcgtcgacgc gggtgagtgc ccgagtgcgc cgcgacttga tccgtcgacg aagtgctcaa
    18241 ccagcgcggc gatgagcgcg cggtaggcgg ggccttccga ggggtgcggc agggcggcat
    18301 accggtatgc cggtcgcccc cgccccgcgg gtgcgtcgcg atcgcgcgcc gccagcccga
    18361 ggtcgaccaa ggcatcgagg tgttcgcgca ccgtgttggg gtgctgtccc gtccgcgcgc
    18421 ccaactcggc ggcggtcgcg ggcccgtcgg cgctcagcgc ggcaaggacc tccccctggg
    18481 cgggggtagc gacggccgtc ggttcctgcg tcggcgccgg cccgagaacg ggcgatcggt
    18541 acggggtatt taacacggct ctaagcgtac tatccaagaa cagtccaggg aa
//
LOCUS       DAORPW010000036        17983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_573_length_17983_cov_637.219824, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000036 DAORPW010000000
VERSION     DAORPW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 17983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17983
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_573_length_17983_cov_637.219824"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /gene="der"
                     /locus_tag="PLB86_04840"
     CDS             complement(<1..920)
                     /gene="der"
                     /locus_tag="PLB86_04840"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRC64494.1"
                     /translation="MAVERALRAGLADFELDETDLDLLEHGGDAAYGDAFAGPLPVAA
                     IIGRPNVGKSTLVNRILGRREAVVEDVPGVTRDRVAYEAEWAGRRFTLVDTGGWEVDA
                     SGIHLRVAEQAEIAIELADAVMFVVDAVVGATDTDEAVVKLLRRSGKPVVLVANKVDD
                     QRFESDATALWGLGLGQPWPVSALHGRGSGDALDALMAVLPERSMVGGAYERGGPRRV
                     ALIGRPNVGKSSLLNKVAGSERVVVDSVAGTTRDPVDELVEIAGKQWRFVDTAGIRRR
                     VHQTRGADFYASLRTQTALEKAEVAVVLIDA"
     gene            complement(1031..1705)
                     /locus_tag="PLB86_04845"
     CDS             complement(1031..1705)
                     /locus_tag="PLB86_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC64495.1"
                     /translation="MADVLLAPPAPWRPRAGRWLGIAIFHTAYRGVAIDAHLVPRTGP
                     VVLVANHSGFLDGPLVFSSTPRPVSFLVKQETFHGPLGTLLRGVGQIPVDRGVGDRAA
                     LGAASAVLARGGAVGVFPEGTRGRGDVAQVNQGATWLAVQSGAAVVPVACLGTRRTGD
                     STESMPRVGTRLVAVFGQPFVIPVEAGVPGRERLRRGSELLRERLSAHVTAAAARMGM
                     PLPEDD"
     gene            complement(1698..2414)
                     /gene="cmk"
                     /locus_tag="PLB86_04850"
     CDS             complement(1698..2414)
                     /gene="cmk"
                     /locus_tag="PLB86_04850"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRC64496.1"
                     /translation="MSASQAAGMPLVVAIDGPSGSGKSSVSRAVATALDAAYLDTGAM
                     YRALTWWCLDQGMDVTDREAVAAVASSAPLEVGMDPDEPRIGVDGNDLTEEVRSVRVT
                     EAVSAIATNLDVRADMRRRQRALIAEGLAARGAVVAEGRDITTVVAPDAPVRILLTAS
                     EDARLARRAQQDFGTADDGAKAATRDRVVARDAADSTVSQFTTAADGVVTIDSSQLDF
                     AQTVAAVLAVVAEARMPSGG"
     gene            complement(2414..3508)
                     /locus_tag="PLB86_04855"
     CDS             complement(2414..3508)
                     /locus_tag="PLB86_04855"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HRC64497.1"
                     /translation="MTTRVLIRGTGLIGTSLGIALTRTGYAVLLADPSPTAERLARDL
                     GAGQLAHESAEQPDLVIIAAPPDVAAGAVAQSLSRWPSAVVTDVASVKGAVVDGARAA
                     GADLTRYVGSHPMAGRERSGAGHARGDLFEGRAWVVVPAPESTESTIQAVTELARAVG
                     SVVTVMDATTHDDAVATMSHLPQIAASLVAAQLRSLDDRALGLAGQGVRDVTRVAASD
                     PRLWTQIIAGNATSILEVLGRVHADLGELLGALSALADPDAPDDAVGSLEVVNRTITH
                     GNVGHARIPGKHGAGPAAYLPVTALVPDTPGELARLLTDVGEAGVNLEDLRIDHGLGQ
                     PFGLAEIMVVPAAAEHLQQALEAKGWRVLD"
     gene            complement(3557..4582)
                     /locus_tag="PLB86_04860"
     CDS             complement(3557..4582)
                     /locus_tag="PLB86_04860"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRC64498.1"
                     /translation="MSAAQSGGRGRSGGQSGGGRPSGGGYGGSRGGSGSSGGSGGSGM
                     PPRGPRPGGGGPRKPTGRPTGSGKAPRFAGSTKPSQPTALPDRKVARPAVPVDVHDPD
                     GVRLQKLLAAAGIGSRRACENLIAAGRVEVDGHVVTELGVRIDPLRQSVHVDGERVQL
                     DESRVYLAFNKPLGVVSTMSDELGRPSISDFLGARQDRLFHVGRLDADTEGLLLLTND
                     GDLAHRLQHPAYGVLKTYLAQVPGPVARDLGKRLRAGIELDDGPVAVDSFKLVDSAPG
                     RALVEVVLHEGRKHVVRRMLAAVGHPVEYLVRTDVGPIRLGDLKVGKTRRLTREEVGK
                     LYTAAGL"
     gene            complement(4579..5235)
                     /gene="scpB"
                     /locus_tag="PLB86_04865"
     CDS             complement(4579..5235)
                     /gene="scpB"
                     /locus_tag="PLB86_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRC64499.1"
                     /translation="MSEDLRVDDEAVPPDDAGSAEETEAAGQVAFDVEAFPGGARSAI
                     EAVLMVVDEPVSEVALATALEVPLADVRAHLEGLEAEYAAANRGFTLRPFGGGWRVYS
                     RSDYAPAVEKFLLDGQQARLTQASLETLAVIAYKQPVSRSRIGAVRGVNVDGVVRTLL
                     SRGLIEERGEAEGGAILYGTTPYFLQRLGLDSLDDLPPLAPYLPDVDMLDEIAEAGRR
                     "
     gene            complement(5232..6161)
                     /locus_tag="PLB86_04870"
     CDS             complement(5232..6161)
                     /locus_tag="PLB86_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HRC64500.1"
                     /translation="MTDALLPEAPATPGGVLTRRTSSTPFEVHLDVFEGPFDLLLGLI
                     AKHKLDITEIALARVTDEFIGHIRTIREADEEWDLSQASEFLLVAATLLDLKAARLLP
                     QADPDDDEDLALIEARDLLFARLLQYRAFKDIAATFAEQLATTGRLTPRQAGLEPRFA
                     ALLPELVMGITPEQLAMIAARAMIPKAPPTVALEHLHAAQISVRDQAAILVTRLRVER
                     TCSFRTLVADADSTLVIVARFLALLELYKEGAITFDQAEPLADLAVRWTGSDEGDIAV
                     AAEFDEDDAQLDFDDAGTDTGAASGIPMQGADE"
     gene            complement(6222..7037)
                     /locus_tag="PLB86_04875"
     CDS             complement(6222..7037)
                     /locus_tag="PLB86_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC64501.1"
                     /translation="MPSFPTPPPLTSHGPARVIAMCNQKGGVGKTTTTINLGAALAEL
                     GRKVLLVDFDPQGALSVGLGIRPNDLDITIYNLLVERGHDIRDVIQHTRSENLDLIPA
                     NIDLSAAEVQLVGEVAREQILARALRPVMDDYDVIIIDCQPSLGLLTVNALTAAHGVF
                     IPLECEFFAMRGVALLVETIEKITDRLNPRLQVDGILATMYDGRTLHSREVVKSVVDH
                     FGDRVFHTVISRTVKFPDASLAAEPITSYASTSAAAAAYRQLARELIARGGAA"
     gene            complement(7230..8177)
                     /gene="xerD"
                     /locus_tag="PLB86_04880"
     CDS             complement(7230..8177)
                     /gene="xerD"
                     /locus_tag="PLB86_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRC64502.1"
                     /translation="MSTIPTPAVSRALARAVRGWLDHLDVERGVARNTLVSYTRDLDR
                     YLDYLARQGVSTPENVTEAHVSGFLADLRAGSPGRMPLSASSAARTLVAVRGLHRFLA
                     LEGRTLADPAEGVRPPKTPLRLPKAIGVDDVERLLAAASLGDTPVSMRDRALFEVLYG
                     AGARISEAVGLDVDDLDLESGTVRLFGKGSKERIVPVGRYAREAVAAYLVRGRPALAA
                     RGKGTPALFLGTRGARLSRQTAWSALAAAGERAGLAGHLSPHTLRHSFATHLLEGGAD
                     VRVVQELLGHASVTTTQIYTMVTVARLREVYAESHPRAR"
     gene            complement(8174..10024)
                     /locus_tag="PLB86_04885"
     CDS             complement(8174..10024)
                     /locus_tag="PLB86_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRC64503.1"
                     /translation="MGTLITTLSSAAPRMTPGERRLAQRLQQKLDADYLVWFDVPVGP
                     MRTHPDFVVLHPSRGVLILEVKDWALPLIQRVDREMWDIQPDGRHKRVINPILQARHG
                     AHDVVRALERDLQLVHEAGAHQGKLAFPWGYGVVFTKIDRAEFDAAGLGEVIDGHAVM
                     CADEMTESVDPEAFQERLWGMFPRRFPAKLTLPQIDRVRWIMFPDVRVDPPVASLFDA
                     AASPDSPEGDAGAAAAIELPDVMKVMDLQQEQLARSLGAGHRVVHGVAGSGKTMILGY
                     RAEYLARLVAPGAKPVLVLCFNEPLGAALSANFASRRLADRVQVRHFHRWARQQLVTF
                     GQPLPPDGPHFFDDLVGYLIRAVDRGHVPRAQYSAVLIDEGHDFRPEWLHLVVQMVDP
                     ETNSLLLLYDDAQNIYGRGRTPAFSFKSVGIQAVGRTTILRVNYRNTTQILRLAQRVA
                     GDILKPQACDEDGAPLLGALGCGRDGEEPDVRRLPTLGAEAAYVAQRFAAAARDGRAW
                     SDMAVLCHDTAVMDACASALRRVRIPHSVRRRPGDFRPEADTVKILTMRVSKGLEFPV
                     VAIPGVGRLPAPHEDDKDAARLFYVAATRATERLLVTVSGDGSLGQRLGV"
     gene            10134..10742
                     /locus_tag="PLB86_04890"
     CDS             10134..10742
                     /locus_tag="PLB86_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64504.1"
                     /translation="MKLYADLPGRRAAQVVADVLSVGWILACTNIGKAVHDATMGLRQ
                     PADGLASAGNQLEAGMTSAGDRIAQVPLVGDALQGPFRDAAGTGVALAQVGTDLSGSI
                     GRVALIAGLVVAILPILLWMLVWLPLRIRFIRRASDAANFIEGRADLDLFALRALVRV
                     PTKRLATIHADPAAAWRSRDPHVIRELANLELLQCGVRPPRA"
     gene            complement(10774..12123)
                     /gene="hisS"
                     /locus_tag="PLB86_04895"
     CDS             complement(10774..12123)
                     /gene="hisS"
                     /locus_tag="PLB86_04895"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRC64505.1"
                     /translation="MSTGKVTPISGFLEFLPEQRIVEQHFLDVIREVFELHGFASIET
                     RAVESVERLLGKGGDADKEIYGISRLAADASDSREPSLGLHFDLTVPFARYVLENAGK
                     LAFPFRRYQMQKSWRGERPQEGRYREFTQCDIDVVDVGSLSPQYEIEMPLVLTDVFDR
                     LPIGDYVVQVNDRRIPQGFYRGLGIEDIGGTLRIVDKLDKLPADTVRTMLLDSGCTAE
                     QADQCLALAGIRTEDLSFIEQVRALGVADPLIDEGLTGLAAVVDAGRTHAPGRVVADL
                     KLARGLDYYTGTVYEVRLLGFESWGAVAGGGRYDALATDGTVTYPGVGISIGVSRLLG
                     LLFRQGLVSASRSTPACVLVALTDEESRPRSLAVASALRGRGIPCEVAPRPDKFGKQI
                     RYAARRGIPYVWFPGGPESDGDSVKDIRSGEQVPATAVDWAPPAADLRPSVVRAAAD"
     gene            complement(12226..12921)
                     /locus_tag="PLB86_04900"
     CDS             complement(12226..12921)
                     /locus_tag="PLB86_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC64506.1"
                     /translation="MLMIGFPAAAFDTNCFVLATAEGEECVVVDPGIGVTDTLSEVLR
                     EFRLRPAAVLLTHGHLDHVYSVTPVCRGGGAAAYIHGDDRYRLRDPLADLDRGMIAMM
                     EAQFGERATWTEPDDIRELRDGDSVTIAGMDFGIVHAPGHTEGSVMFTLAEVPEGINV
                     EVGSTVISGDVLFAGSIGRSDLAGGDPAAMNRSLRDCVLPMPDDRLVLPGHGPLTTIG
                     RERASNPYLRGLR"
     gene            13056..13847
                     /locus_tag="PLB86_04905"
     CDS             13056..13847
                     /locus_tag="PLB86_04905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC64507.1"
                     /translation="MRDKQPVLAVIGVLLAVIAFVWVANVVTAPKVADPTATGEPTST
                     ATTTATDNPSATATATPTPTPTPKVLAGCTEVPAPQAAPQKMTTAPDIEAARGKTFIA
                     TVTTNCGDVTIELDGTKAPATVSSFVLLGNAGYWAPSPCHRLTGDAQGLWVLQCGDPT
                     GTGAGPGPGYHYGIENAPGDGKYPAGTVAMARTSDPNSNGDQFFIVYKDTTLPVDGGG
                     YSIFGKVTGGMDIVDRIAAAGVSPADGMSPMAPISILSMTVKAKG"
     gene            13850..>14257
                     /locus_tag="PLB86_04910"
     CDS             13850..>14257
                     /locus_tag="PLB86_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64508.1"
                     /translation="MTESPLHESDAPDAPDVSDEPIALEAEVEAPVTVPEPDETVIEA
                     AAEDIVAEAPAEEAEEPAAEDSVAEVVDEVVAEEAPAIDVAEDVTVEVVDEVVAEEAP
                     AIEPVVEDVVEPVVEAVVEPVVEDVTVEVVDEVA"
     assembly_gap    14258..14357
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14358..15771
                     /locus_tag="PLB86_04915"
     CDS             <14358..15771
                     /locus_tag="PLB86_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HRC64509.1"
                     /translation="VVEPVVEDVTVEVVDEVAEAEEVAASADAEATAAATPPPAPSPA
                     LFARSAGAPATSAFGRVGTDGVVFVKTADGEREVGSYPGVSNADALAYFGRKYDELAA
                     SLHLLQQRVTHTEMPAKEAADAVSKLRAQVVEARVVGDLAALDRQLDEIDAAIVVRRE
                     ADTVARAAAKLAATTQREALVAEAEQISGQPEQKIQWKSSGARMRELLDEWKTHQRSG
                     PRLDKDVEAGLWQRFSVARNSFDKARRTHFAALDSAHDEARTIKERLVAEAERLATSR
                     DWMSTAGAFKHLMDDWRAAGRAGRAEDDALWLRFKSAQDTFFNAKDAIVAAEEEGFRA
                     NLTVKQALIVEAEAILPVTDLEKAKAALRIIQDKWDKAGKVPRADMERTEKAIRRVEQ
                     AVRDAEDRRWTASNPEAAARAKSLVEQLEKAVTSLEKDLASAQASGNAKKVADAQAAL
                     DARRQWLDQARSGLAEFGGR"
     gene            15896..16117
                     /locus_tag="PLB86_04920"
     CDS             15896..16117
                     /locus_tag="PLB86_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HRC64510.1"
                     /translation="MTEPLPRPDSQDPGAAFAAVVALRRKANRLEVDTVVEAIGQGWT
                     WAAVGEALGISAQAAHKKFAATVRARAAR"
     assembly_gap    16217..16316
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16317..>17983)
                     /locus_tag="PLB86_04925"
     CDS             complement(<16317..>17983)
                     /locus_tag="PLB86_04925"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRC64511.1"
                     /translation="HRLGMNTIKWELEDLAFQALYPKMYDEIVDLVAARAPAREEFLA
                     RVCSEVAEDLKVAGIKATVTGRPKHYYSVYQKMIVRGRDFEEIYDLVGVRVLVETVRD
                     CYGALGSLHARWNPVPGRFKDYIAIPKFNMYQSLHTTVIGPQGKPVEIQIRTHTMHRR
                     AEYGVAAHYKYKEDHKRGGRGAPVESDSLAETAWLRRLLDWQREIADPGEFLDSLRFD
                     LGGGEVFVFTPKGAVVSLPAGSTPVDFAYAVHTEVGHHCIGGRVNGRLAPLDSVLENG
                     DSVEILTSKADGAGPSRDWLAFVKSPRARNKIRQWFTKERREEAIEQGKDAIAKYLRK
                     QGLPLHRLMSAESLTAVAGDLRYSDIDSLFAAVGDSNVSAQHVVSRLVATLGGEEATS
                     EDLAEATVPSRARSRVRSGDPGVVVVGTDDVWVKVARCCTPVPGDAIIGFITRGNGVS
                     VHREDCTNADALRREPDRLIEVAWDPSAASVFLVQLQVEALDRNRLLSDITRAISDHH
                     VNILAASVQTTRNRVATSRFTFEMGDPGHLDHVIAGILKVEGVYDVYR"
BASE COUNT         2761 a         6197 c         6253 g         2572 t
ORIGIN      
        1 gcgtcgatga gcacgacagc cacctcggcc ttctccagcg ccgtctgggt gcgcagcgag
       61 gcatagaagt cggcgccgcg ggtctggtgg acccgccgcc ggatccccgc cgtgtcgacg
      121 aaccgccact gcttcccggc gatctcgacc aactcatcga ccgggtcccg cgtcgtcccg
      181 gccaccgagt cgacgacgac ccgctccgac ccggccacct tgttgagcaa cgacgacttg
      241 ccgacgttgg ggcgaccgat gagggctacc cggcgcggac cgccacgctc atacgccccg
      301 ccgaccatcg agcgctcggg caacacggcc atgagcgcgt cgagcgcgtc gccgctcccc
      361 cgcccgtgca gggcggagac cggccacggc tgccccaacc cgagccccca cagggccgtc
      421 gcgtccgact cgaatcgctg gtcgtcgacc ttgttggcca cgaggacgac cggcttgccc
      481 gagcggcgca gcaacttgac gacggcttcg tcggtgtcgg tcgcgccgac gacggcgtca
      541 acgacgaaca tcacggcgtc ggccagctcg atggcgatct cggcctgctc ggcgacccgc
      601 aggtggatgc cggaggcgtc gacctcccag ccgccggtgt cgacgagggt gaaccgccgc
      661 ccggcccact cggcctcata ggccacccgg tcccgcgtca ctcccggcac gtcctcgacg
      721 accgcctcac ggcgcccgag gatccggttg acgagggtgg acttgccgac gttggggcgc
      781 ccgatgatcg ccgccaccgg gaggggcccg gcaaaggcat ccccgtatgc cgcgtcgccg
      841 ccgtgctcga gcaggtccag gtcggtctcg tcgagctcaa agtcggccag cccggcacgc
      901 agggcgcgct cgactgccag ctcgtcaggg gtcgtcgcgg ccgattcgga gggggcgagc
      961 tcaagctcgg ggtcggtggg gttctgcggg ttcacccccg gattctccca cctcgccgac
     1021 gacgcgcggt tcagtcgtcc tcggggagcg gcatacccat ccgggcggcc gcagcggtga
     1081 catgggccga caggcgctcg cgcagcagtt ccgagcccct gcgcagccgc tcccgaccag
     1141 gcactccggc ctcgacgggg atgacgaacg gttggccgaa gacggcgacc aatcgggtgc
     1201 cgacccgggg catcgactcg gtgctgtccc ccgtgcgccg ggtgcccaga caggcgacgg
     1261 gcacgacggc ggcccccgac tgcaccgcca accacgtagc gccctggttg acctgggcga
     1321 cgtcgccgcg accgcgggtg ccttcgggga acaccccgac cgctcccccg cgcgccagca
     1381 ccgcgctggc cgctccgagg gccgcacggt cccccacacc ccgatcgacg gggatctgcc
     1441 cgaccccgcg cagcaaggtc cctagcgggc cgtggaaggt ctcctgcttg acgaggaacg
     1501 acaccggccg cggggtgctg ctgaagacga gcggcccgtc gaggaaccca ctgtggttgg
     1561 cgaccaggac gaccggcccc gtgcgcggca cgaggtgcgc gtcgatcgcc acccctcggt
     1621 atgccgtgtg gaaaatcgcg atgcccagcc accgtccggc gcggggccgc cacggcgcgg
     1681 gaggcgccag gaggacgtca gccaccggaa ggcatccggg cctcggcgac caccgcaagc
     1741 actgcggcca cggtctgcgc gaaatcgagt tgggaactgt cgatcgtcac cacgccgtcg
     1801 gcggcggtgg tgaactgcga caccgtcgag tcggcggcat cgcgcgccac cacccgatcg
     1861 cgggtggctg ccttggcccc gtcgtctgca gtgccgaaat cttgttgggc gcggcgggcc
     1921 agtcgggcgt cctcgctcgc ggtgaggagg atccgcaccg gcgcgtccgg ggcgaccacc
     1981 gtggtgatgt cgcggccttc agcgacgacg gcgccccggg ccgcgaggcc ttcggcgatg
     2041 agggcccgtt gccgccggcg catgtcggcc cgcacgtcga ggttggtggc gatggccgac
     2101 acggcctcag tgacccggac cgagcggacc tcctcggtga ggtcgttccc gtcgaccccg
     2161 atccgaggct cgtccgggtc catccccacc tcgagcggtg cgctcgacgc cacggcggcg
     2221 acggcctccc gatcggtgac gtccatcccc tggtcgaggc accaccaggt gagcgcgcgg
     2281 tacatggcac cggtgtcgag ataggcggca tccagcgccg tggcgaccgc ccgcgacacc
     2341 gacgacttgc ccgagccgga cgggccgtca atggcgacga cgagcggcat acccgccgct
     2401 tgtgaggcgc tcatcagtcg agcacccgcc agcccttggc ctccagcgcc tgctggaggt
     2461 gctcagcggc ggccgggacg accatgatct cagcgaggcc gaagggctgc ccgaggccgt
     2521 ggtcgatgcg caggtcctca aggttgaccc ccgcctcacc gacgtcggtg agcagccggg
     2581 ccaactcgcc cggcgtgtcg gggacgagcg ccgtgaccgg caggtatgcc gcgggcccgg
     2641 ccccgtgctt gccagggatc cgcgcgtggc cgacgttgcc gtgcgtgatg gtgcggttga
     2701 ccacctcaag gctgccgacg gcgtcgtcgg gtgcgtcggg gtcggcgagg gcgctcaggg
     2761 cgcccagcaa ctcgcccagg tcggcgtgga cccgcccgag gacttcgagg atcgacgtgg
     2821 cgttgccggc gatgatctgc gtccacaacc gcgggtcgct cgcggcgacc cgggtgacgt
     2881 cgcggacgcc ctgcccggcc aggcccagcg cccggtcgtc cagcgaacgc agttgggctg
     2941 caacgagact cgctgcgatc tgcgggaggt gcgacatcgt tgccacagcg tcgtcgtggg
     3001 tggtggcgtc catgacggtg accaccgacc cgacggcccg cgccagctcc gtcacggcct
     3061 ggattgtcga ctcggtcgac tccggggcag gcacgaccac ccaggcccgg ccttcgaaga
     3121 ggtccccgcg ggcgtgcccg gcgccggagc gctcgcggcc ggccatcgga tgggacccga
     3181 cgtagcgggt gagatcggcg cccgcagccc gagcaccatc gacgacggca cccttgaccg
     3241 acgcaacgtc cgtgacgact gcagacggcc aacgggacag cgattgtgcg acggcgccgg
     3301 ccgcgacatc cggcggggca gcgatgatga cgagatcagg ctgctccgcg ctctcgtggg
     3361 cgagttgccc ggcgccgagg tcgcgggcca gccgctcggc ggtcggcgag gggtcggcga
     3421 ggagcacggc ataccccgtg cgggtgagcg cgatccccag gctggtgccg atgagcccgg
     3481 tgccgcggat gagcacgcgc gttgtcacgg cggtcacgct accgagcggt cggcgcggca
     3541 gcgtgggccg tccgcatcac agacccgcag cggtgtagag cttgccgacc tcttcgcggg
     3601 tcaaccggcg ggtcttgccg accttgaggt cacccaaccg gatcgggccg acatcggtgc
     3661 gcaccaggta ttcgaccggg tgcccgacgg cggcgagcat ccggcgcacg acatgcttgc
     3721 gcccctcatg caggaccacc tcgacgagcg cgcggccagg cgccgagtcg acgagtttga
     3781 acgagtcgac tgcgaccggc ccgtcgtcca actcgatccc ggcgcgcagc cgcttcccca
     3841 ggtcgcgggc cacggggccc ggcacctgcg cgagataggt cttgagcacg ccgtatgccg
     3901 ggtgctgcag tcggtgggcg aggtcgccgt cgttggtgag cagcaacaga ccctcggtgt
     3961 cggcatccaa ccggccgacg tggaagagcc ggtcctggcg ggcaccgagg aagtcggaga
     4021 tcgacgggcg acccaactcg tcgctcatcg tcgagacgac gcccaggggc ttgttgaagg
     4081 cgaggtagac ccgcgactcg tcgagttgga cccgctcgcc gtcgacgtgg accgactggc
     4141 ggagggggtc gatgcggacc ccgagctcgg tgacgacgtg accgtcgacc tcgacacggc
     4201 cggccgcgat gaggttctcg caggcccggc gtgacccgat ccccgcggcg gccaggagct
     4261 tctgcaggcg caccccatcg ggatcgtgaa cgtccaccgg aacggccggg cgggccacct
     4321 tgcggtcggg cagggccgtc ggctggctgg gcttggtgga gccggcgaag cgaggggcct
     4381 tgccgctgcc cgtcggtcgt cccgtgggtt tgcgcgggcc gcctcccccg gggcgagggc
     4441 cccgcggcgg cataccggat ccacctgagc cgccggaact gcccgaaccg ccgcgagagc
     4501 cgccgtagcc cccgccggag ggacgaccac caccggactg gccgccggag cgaccccgcc
     4561 cacctgactg tgccgcgctc atcgtcgacc tgcttccgca atctcgtcca gcatgtccac
     4621 gtcgggcagg tagggcgcca acgggggcag atcatcgagc gagtccagcc cgaggcgctg
     4681 caggaaatag ggcgtggtgc cgtagaggat cgccccgccc tcggcctcgc cgcgctcctc
     4741 gatcaggccg cgcgacaaca gggttcgcac gacaccgtcg acgttgacgc cgcgaacggc
     4801 accgatgcgc gagcgcgaga ccggctgctt gtaggcgatg accgccagcg tctccagcga
     4861 cgcctgggtc agccgggcct gctgcccgtc gaggaggaac ttctcgacgg ccggggcgta
     4921 gtcggaccgg ctgtagaccc gccacccgcc gccgaacggc cgcagggtga agccgcggtt
     4981 cgcggcggca tactcagctt ccaggccctc caagtgggcg cggacgtccg ccaggggcac
     5041 ttcgagggca gtcgccaacg cgacctcgct cacgggctcg tcgacgacca tgaggacggc
     5101 ttcgatcgcc gaccgcgccc cgccggggaa cgcctcaacg tcgaaggcca cctggccggc
     5161 agcctcggtc tcctcggctg acccagcgtc atccggtggc accgcctcgt cgtccacgcg
     5221 gaggtcctca ctcattcgtc tgctccttgc atcggtatgc cgctcgccgc gccggtgtcg
     5281 gtccccgcgt cgtcgaagtc caactgcgcg tcgtcctcgt cgaactcggc ggcgaccgcg
     5341 atgtcgccct cgtccgaccc ggtccagcgc acggcgaggt cggccaacgg ctcggcctgg
     5401 tcgaaggtga tcgcaccctc tttgtaaagc tcaagcagcg ccaggaaccg cgccacgatc
     5461 accagtgtgg agtcggcatc ggcgacgagg gtccggaacg agcaggtccg ctcgaccctc
     5521 agccgggtga cgaggatggc cgcctggtcg cgcacgctga tctgggcggc gtgcaaatgc
     5581 tccagcgcga cagtcggcgg ggccttcggg atcatcgcgc gagcagcgat catcgccaat
     5641 tgctcggggg tgatccccat gaccaactcg ggcagcaggg ccgcgaagcg aggctctaga
     5701 ccagcctggc gaggggtcag gcgccccgtg gtggccaact gctcggcaaa ggtcgccgcg
     5761 atgtccttga acgcgcggta ttgcagcagc cgcgcgaaca gcaggtcgcg tgcctcgatg
     5821 agcgccaggt cctcgtcgtc gtcggggtcg gcctgcggca gcaggcgggc cgccttgaga
     5881 tccagcagag tggccgcgac cagcaggaac tcggaggcct gcgagaggtc ccactcctcg
     5941 tcggcctcgc ggatggtgcg gatgtgccca atgaactcgt cggtcacgcg ggccagggcg
     6001 atctcggtga tgtcgagttt gtgcttggcg atgagcccca gcagcaggtc gaaaggcccc
     6061 tcgaagacgt ccaagtggac ctcgaagggc gtgctcgagg tgcgccgggt cagcacccca
     6121 ccgggtgtcg cgggggcctc gggcaggagc gcgtcggtca cgggatgact gtcatgggta
     6181 tcacggtcat gggttcggcg tccgcaggcg gatgcctcgg ctcaggccgc gccgccgcgg
     6241 gcgatgagct cgcgggccag ttgccggtat gccgcggcag cagcactcgt ggaggcgtag
     6301 ctcgtgatcg gctcggccgc caggctggcg tcggggaact tcacggtgcg gctgatgacc
     6361 gtgtggaaga cccgatcgcc gaagtggtcg acgaccgact tgacgacctc tcggctgtgc
     6421 agcgtgcgcc cgtcatacat cgtggcgagg atgccgtcga cctgcaggcg ggggttgagt
     6481 cgatcggtga tcttctcgat ggtctccacg agcagggcga cgccgcgcat cgcgaagaat
     6541 tcgcactcca gggggatgaa gaccccgtgg gcagccgtca gcgcgttgac ggtgagcagc
     6601 ccgagcgagg gctgacagtc gatgatgatg acgtcgtagt cgtccatgac cgggcgcaag
     6661 gcccgagcca ggatctgctc gcgggccacc tcgccgacga gttgcacctc ggcggccgac
     6721 aagtcgatgt tggccggaat gaggtcgagg ttctccgagc gggtgtgctg gatgacgtcg
     6781 cggatgtcgt ggccgcgctc gacgagcagg ttgtagatcg tgatgtccag gtcgttcggc
     6841 cggatcccca gacccaccga cagggcgccc tgcgggtcga agtcgacgag gagcaccttg
     6901 cggccgagct ccgcgagggc ggcaccgagg ttgatggtcg tggtcgtctt gccgaccccg
     6961 cccttctggt tgcacatggc gatgacgcgg gccggcccgt ggctggtcag cgggggtggc
     7021 gtggggaagg acggcatggg gcgtccggtc ggcccggtgc ctccgatccc ggcgtcttcc
     7081 gtgcccggca gcagggtgtc ggtggcgtcc cgcaggtcgg ggaccatgtg gacggcttcg
     7141 ccgggaaggt tgcgatcgat gctcacagct gcgcctcttc cccgtcgttc gtgcggtcgg
     7201 tcagcctctc gctgatcggt gcgaccttat cagcgggccc gggggtgcga ctcggcatac
     7261 acctcgcgca gcctcgccac ggtgaccatt gtgtagatct gggtggtcgt caccgaggcg
     7321 tgtccgagca actcctgcac cacccggacg tcggcgccgc cttcgagcag gtgggtggcg
     7381 aacgagtgcc gcagcgtgtg cggcgacaag tggccggcca ggcccgcccg ctcccctgcc
     7441 gctgccagcg ccgaccacgc ggtctggcgg ctgagccgcg caccccgggt gccgaggaac
     7501 agggccggag tgcctttccc gcgggccgct agcgccggtc gcccccgcac gaggtatgcc
     7561 gccaccgcct cccgtgcgta gcgccctacg gggacgatcc gctccttgga ccccttgccg
     7621 aagagccgaa cggtgcccga ctccaggtcg agatcatcga cgtcgagccc gacggcctcc
     7681 gagatccgcg ccccggcgcc atagaggacc tcgaacaagg cccggtcgcg catcgagacg
     7741 ggggtgtccc ccagtgacgc ggcggcaagc agccgttcga cgtcgtcgac accgatggct
     7801 ttaggcaacc gcagcggggt cttgggcggt cgcacgcctt cggccggatc ggccagggtg
     7861 cgcccctcca acgcgaggaa ccgatgcagc ccccgtactg cgacgagggt gcgagccgcg
     7921 gacgaggccg agagcggcat acgccccggt gagcccgctc gcaggtcggc gaggaagccg
     7981 gacacgtgtg cctcggtgac gttttcaggc gtggaaacgc cttggcgcgc aaggtaatcc
     8041 aggtagcggt cgaggtcgcg ggtgtaggag accagggtgt tgcgggcgac tccccgctcg
     8101 acgtcgaggt ggtcgagcca ccctcgcacc gcgcgcgcca gcgcccgcga gacggctggc
     8161 gttgggatcg tgctcatact ccgagccgtt ggcccaggga gccgtcgccg ctgaccgtga
     8221 cgagcagccg ctcggtcgcc cgggtggcgg cgacgtagaa cagccgcgcc gcgtccttgt
     8281 cgtcctcgtg aggggcgggc aggcggccca ccccggggat tgccacgacg gggaactcca
     8341 accccttgct cacgcgcatg gtgaggatct tgaccgtgtc ggcctcggga cggaagtcgc
     8401 cgggccgacg tcgcaccgaa tgcgggatcc gaacccgccg tagcgccgac gcacacgcgt
     8461 ccatgacagc ggtgtcgtgg cacagcacgg ccatgtccga ccaggcccgt ccgtcccgcg
     8521 ccgccgcggc aaacctctgg gcgacgtatg ccgcctccgc accgagcgtg ggcagccgcc
     8581 ggacgtcggg ctcctccccg tcccgcccgc atccgagggc gccgagaagc ggggcgccgt
     8641 cctcgtcgca tgcctgtggc ttgaggatgt cgcccgccac ccgttgggcc aggcgcagga
     8701 tctgcgtggt gttgcggtag ttgacccgca ggatcgtcgt ccggccgacc gcctgaattc
     8761 ccacgctctt gaagctgaac gccggggtgc gcccccggcc gtagatgttc tgggcgtcgt
     8821 cgtagaggag cagcaggctg ttggtctcgg ggtcgaccat ctgcacgacg aggtgcagcc
     8881 actcggggcg gaagtcatgg ccttcgtcga tgaggacggc ggagtattgc gccctcggca
     8941 cgtggccacg gtcgacggcc cggatgaggt agccgacgag gtcgtcgaag aagtgtggtc
     9001 cgtcgggtgg cagcggctga ccgaaggtga ccagttgttg gcgggcccag cggtggaagt
     9061 gtctgacctg cacgcgatcg gccaggcgcc gcgaggcgaa gttggccgac agcgcggcac
     9121 cgagcggctc gttgaagcac aacaccagca cgggcttggc gccaggggcg acgaggcggg
     9181 caaggtattc ggctcggtag cccaggatca tcgtcttgcc cgacccggcc accccgtgga
     9241 ccacgcggtg cccggccccg aggctgcgtg ccaactgctc ctgctgcagg tccatgacct
     9301 tcatgacgtc gggcagttcg atggccgcgg ccgcgcctgc gtccccctcg ggcgagtcgg
     9361 ggcttgccgc ggcgtcgaag agcgaagcaa caggtgggtc cacccgcacg tcgggaaaca
     9421 tgatccaccg cacccgatcg atctgcggca gcgtgagttt cgccgggaag cgccgaggga
     9481 acatccccca caaccgctcc tggaacgcct cagggtcgac agattcggtc atctcgtcgg
     9541 cgcacatgac cgcgtgcccg tcgatcacct cgcccagccc ggccgcgtcg aactccgcgc
     9601 ggtcgatctt ggtgaagacc acgccgtagc cccacgggaa ggcgagcttc ccctgatgcg
     9661 ctccggcctc gtggaccaac tgaaggtcgc gctccagagc ccgcacgacg tcgtgagccc
     9721 cgtgtctggc ctgcaggatc gggttgatca cccgcttgtg tcgcccgtcc ggctggatgt
     9781 cccacatctc ccgatcgacc cgttggatga ggggcagagc ccagtccttg acctcaagga
     9841 tgagcacccc tcgactcggg tgcaggacaa cgaaatcggg gtgggtgcgc atcgggccga
     9901 ccggcacgtc gaaccacacg agatagtccg cgtcgagctt ctgctgcaag cgttgggcca
     9961 gccgacgctc gcccggtgtc atccgcgggg cagcgctgct caacgtcgtg atcaacgtgc
    10021 ccatgctcgg caggctagtc cgcgtggacg gcgacgcaac cgcaggcgac caccagagcg
    10081 acggcgtagc gtgagcctga cactgcctgc gttgccctgg tgaggagatc cccatgaagc
    10141 tgtatgccga tctgcctggg cgccgcgccg cccaggtggt cgctgacgtc ctcagcgtgg
    10201 gctggatcct cgcctgcaca aatatcggga aggccgtcca cgacgcgacc atgggcctgc
    10261 gccaacccgc cgacggactg gcgtcggccg ggaaccagtt ggaggcgggc atgacctctg
    10321 cgggcgaccg gatcgcccag gttccgctcg tcggcgatgc cctccaagga cccttccggg
    10381 atgccgccgg caccggtgtc gcgctggccc aggtcgggac cgatctcagc ggctcgatcg
    10441 gtcgcgtggc cctgatcgcc ggtctcgtgg tggcgatcct tccgatcctg ctgtggatgc
    10501 tggtgtggct gccccttcgc atccggttca tccgccgggc cagcgatgca gccaacttca
    10561 tcgagggtcg ggccgacctg gacctgttcg ccctgcgagc actggtgcgg gtgcccacga
    10621 agcggttggc cacgattcac gccgatcctg ccgctgcgtg gcgatccagg gacccccacg
    10681 tgatccgtga gttggccaac ctcgagttgc tccagtgcgg agttcgcccg ccccgcgcct
    10741 gatcaggcgc tgaccggctg tcgcgttggt cggtcagtcc gccgcggcgc gcaccacgga
    10801 agggcgcaga tccgccgccg gaggtgccca gtccaccgcc gtcgccggga cctgctcgcc
    10861 cgagcggatg tccttgaccg agtcgccgtc cgactccgga ccaccgggga accagacgta
    10921 agggatgccg cggcgcgcgg cataccggat ctgcttgccg aacttgtcgg ggcgcggcgc
    10981 gacctcgcac ggtatgccgc gtccgcgcag tgccgaggcg acggccaacg accggggccg
    11041 ggactcctcg tcggtgaggg cgaccaggac gcaggccggg gtcgaccggg aggcgctcac
    11101 caagccctga cggaacagca ggccgagcag tcgggagacg ccgatggaga tgccgacacc
    11161 cggataggtc acggtgccgt cggtggccag cgcgtcgtag cgccctcccc cggcgacggc
    11221 tccccaggac tcgaagccaa gcaggcgcac ctcgtagacc gtgccggtgt aatagtccag
    11281 gccacgggcg agcttgaggt cggcgacgac cctcccgggg gcgtgggtgc gcccggcgtc
    11341 cacgacggcc gccaacccag tcagtccctc gtcgatgagc gggtcggcca cgccgagcgc
    11401 ccggacctgc tcgatgaagg acagatcctc ggtgcggatc ccggccagcg cgaggcactg
    11461 atcggcctgc tcggcggtgc agccggagtc gagcaacatc gtccggaccg tgtccgcagg
    11521 gagcttgtcg agcttgtcga cgatccgcag ggtgcccccg atgtcctcga tgcccaggcc
    11581 tcggtagaac ccctgcggga tccgacggtc gttgacctgc acgacgtagt cgccgatggg
    11641 cagccggtcg aagacgtcgg tgaggaccaa cggcatctcg atctcgtact gcggcgacag
    11701 cgaccccacg tcgacgacgt cgatgtcgca ctgggtgaac tcgcggtagc gcccctcctg
    11761 aggccgctcg ccgcgccagg acttctgcat ctgatagcgc cggaacggga acgcgagctt
    11821 gccggcgttc tccaggacgt agcgagcgaa gggaacggtg aggtcgaagt gcaggccgag
    11881 ggagggctca cgcgagtcgc tggcgtcggc cgccaaccgg ctgatgccgt agatctcctt
    11941 gtcggcgtcg ccgcccttgc cgagcaaccg ctcgaccgac tcgaccgcgc gagtctcgat
    12001 cgaggcgaac ccgtgcagct cgaacacctc acggatgacg tcgaggaagt gctgctcgac
    12061 gatccgctgt tcggggagga actcgaggaa gccgctgatc ggggtgacct tgccggtgct
    12121 caccgaggga tcatcccaga cccggtgtcg ccgtcgcgcg agcgtttcgc cgctgcggcc
    12181 ccatgcgatg gggatgcaag tgctgcgacg gccggggccg atcggtcagc gcagcccgcg
    12241 cagatacgga ttggaggccc gctctcgccc gatcgttgtc aacggtccgt gcccgggaag
    12301 caccagccgg tcgtccggca tcggcaggac gcaatcgcgc agcgaccggt tcatcgccgc
    12361 cgggtcgccc cccgccaggt ccgagcggcc gatcgagccg gcgaagagga cgtcaccgga
    12421 gatcaccgtg gagccgacct cgacgttgat gccttccggg acctccgcca gggtgaacat
    12481 cacggacccc tcggtgtgtc cgggtgcgtg gacgatgccg aaatccatcc cggcgatagt
    12541 caccgagtct ccgtcgcgca attcgcggat gtcgtccggt tcggtccacg tcgcgcgctc
    12601 cccgaactgc gcctccatca tcgcgatcat gccccggtcc agatccgcga gcgggtcccg
    12661 caggcggtag cgatcgtccc catggatgta tgccgcggcg ccgccgccgc ggcatacggg
    12721 cgtcaccgag tagacatggt cgaggtgacc gtgcgtgagc agcaccgccg ccgggcgcag
    12781 ccggaactcg cgcagcacct cggacaaggt gtcggtgacc ccgataccgg ggtcgacgac
    12841 gacgcactcc tccccttcgg cggtcgccag cacgaagcag ttggtgtcga aggccgcagc
    12901 agggaagccg atcatcagca catcgagagc ctatccacgg acaaccttca cgcactcgtg
    12961 accgggggtg gtcagtgtaa gggcctcgcc tgcctagact ggtcgaccgt cggctgtcgt
    13021 cgcgccttgt cggcgcatct aggaggtccg tgctcatgcg cgacaaacaa cccgtgctcg
    13081 ctgtcatcgg cgtgttgctc gccgtgatcg ccttcgtctg ggtggccaac gtggtgacgg
    13141 cacccaaggt cgccgacccg acggccaccg gtgaaccgac cagcacggcg accacgaccg
    13201 ccaccgacaa tccgtcggcg accgccaccg cgacgcccac accgacgccg acccccaagg
    13261 tgttggccgg gtgcacggag gttccggcac cccaggcggc gccccagaag atgacgacgg
    13321 cgcccgacat cgaggcggcc cgcggcaaga ccttcatcgc gaccgtcacc accaactgtg
    13381 gagacgtcac gatcgaactg gacggcacca aggctccggc cacggtgtcc tcgttcgttc
    13441 tcctcggcaa cgccggctac tgggcgccga gcccctgcca ccggctcacc ggcgatgccc
    13501 aaggcttgtg ggtgctgcag tgcggtgacc ccaccggcac gggcgccggt ccgggccccg
    13561 gctatcacta cggcatcgag aacgcaccgg gcgacggaaa gtacccggca ggaaccgtcg
    13621 ccatggcccg cacgagcgac cccaacagca acggcgacca gttcttcatc gtctacaagg
    13681 acacgacgtt gcccgtcgat ggtggcggct actccatctt cgggaaggtc accggaggca
    13741 tggacatcgt cgaccggatc gcggccgcag gcgtcagccc cgccgacgga atgtcgccga
    13801 tggccccgat cagcatcctg tccatgacgg tcaaggcgaa gggctgacga tgaccgagtc
    13861 gccgctgcac gagtccgacg cccccgacgc ccccgacgtc tccgacgagc ccatcgcgct
    13921 cgaagctgag gtcgaggctc ccgtcactgt ccccgaaccc gatgaaacgg tgatcgaagc
    13981 cgccgccgag gacatcgttg ccgaggctcc cgccgaggag gccgaggaac cggctgccga
    14041 ggacagcgtt gccgaggttg ttgacgaggt cgttgccgag gaagcccccg ccatcgatgt
    14101 cgccgaggac gtcactgtcg aggttgttga cgaggtcgtt gccgaggaag cccccgccat
    14161 cgaaccggtc gtcgaagacg tcgtcgaacc ggtcgtcgaa gccgtcgtcg agccggtcgt
    14221 cgaggacgtc actgtcgagg ttgttgatga ggtagccnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnncgt cgtcgagccg gtcgtcgagg acgtcactgt cgaggttgtt
    14401 gatgaggtag ccgaggccga ggaggttgcc gcctctgctg acgcagaagc gacagccgca
    14461 gccactcccc cgcctgcccc gtcgccggcc ctcttcgcac gcagcgccgg cgccccggca
    14521 acgtcggcct tcggacgcgt cggcaccgac ggtgttgtct tcgtcaagac cgcagacggc
    14581 gagcgcgagg tggggtccta ccccggcgtc tcgaacgccg acgccctggc ctacttcggg
    14641 cgcaagtacg acgaactcgc cgccagcctg cacctgctgc agcaacgggt cactcacacg
    14701 gagatgccgg ccaaggaggc cgccgacgcc gtgagcaagc tgcgcgctca ggtcgtcgag
    14761 gcccgtgtcg tcggcgatct cgccgccctg gaccgtcaac tcgacgagat cgacgctgcc
    14821 atcgtcgtgc gccgcgaggc cgacacggtg gctcgggcgg ccgccaaact tgcggcgacc
    14881 acccagcgtg aggcgctcgt cgccgaggcc gagcagatca gcggacagcc cgaacagaag
    14941 atccagtgga agtccagtgg cgcccggatg cgtgagctcc tggacgagtg gaagacccac
    15001 cagcgcagcg gtccgcgcct cgacaaggac gtggaagccg gtctctggca acgcttcagc
    15061 gtggcccgca actcgttcga caaggcgcgt cgcacccact tcgctgcgct cgacagcgcg
    15121 cacgacgagg cgaggaccat caaggagcgg ctcgttgccg aggctgagcg cctggccacc
    15181 agccgcgact ggatgtccac tgccggggcc ttcaaacacc tcatggacga ctggcgcgca
    15241 gcgggccgcg ccgggcgagc cgaggacgat gccctgtggt tgcgtttcaa gtccgcccag
    15301 gacaccttct tcaacgccaa ggacgccatc gtcgcggccg aggaggaagg cttccgggcc
    15361 aacctcacgg tcaagcaggc cctcatcgtc gaggccgagg ccatcctgcc ggtcaccgat
    15421 ctggagaagg ccaaggccgc gctgcggatc atccaggaca agtgggacaa ggccggcaag
    15481 gtcccccgcg ccgacatgga gcgcaccgag aaggccatcc gccgggtcga gcaggccgtg
    15541 cgagatgccg aggaccgtcg ctggaccgcc tcgaaccccg aagccgcagc acgcgccaag
    15601 agcctcgtcg agcagcttga gaaggccgtg acctcgctcg agaaggacct cgcctccgca
    15661 caggcatccg gcaacgccaa gaaggtggcc gacgcccaag ccgcactcga cgcccgtcgc
    15721 cagtggctcg accaggcccg ttcggggctg gcggagttcg gcggccgctg acccacgcgc
    15781 gtccacgacg cggcataccc caggcggggt atgccgcgtc gttgcgttgg gggccgtctc
    15841 cccatcgtca tcaactcagg ttgacgcaca ggtgttatca acgtagattg atgacatgac
    15901 ggagccactg cctcgccctg actcgcagga tcctggggcc gcgttcgcgg cggtcgtcgc
    15961 cttgcgacgc aaggccaacc ggctcgaggt cgacacggtg gtcgaggcga tcggccaggg
    16021 ctggacctgg gccgccgtgg gcgaggcact gggcatcagt gcccaagcgg ctcacaagaa
    16081 gttcgcggcc acggtccggg ccagagcggc ccgctgatcc actggcgatc cgccaggcgc
    16141 ccccgccgac gcatgccgtc ggcaccatga ccacacacag gaggacaacg atgggactgt
    16201 tcaacgccgg ccgggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggt
    16321 agacgtcgta gacgccctcg accttgagga tgccggcgat gacgtggtcg aggtgccccg
    16381 ggtcgcccat ctcgaaggtg aagcgtgagg tcgcgacccg gttgcgggtg gtctgcacgg
    16441 atgcagcgag gatgttgacg tggtgatcgg agatcgcccg ggtgatgtcc gacagcagcc
    16501 ggttgcggtc gagggcctcg acctgcagtt ggacgaggaa gacgctcgcg gctgacgggt
    16561 cccaggcgac ctcgatgagc cggtccggct cgcgccgcag tgcgtcggca ttggtgcagt
    16621 cctcgcggtg gaccgacacg ccgttcccac gggtgatgaa gccgatgatg gcgtcgccgg
    16681 gcaccggggt gcagcagcgg gcgaccttga cccacacgtc gtcggtgccg acgacgacga
    16741 cgcccgggtc gccgctgcgc acccgactgc gggcacgcga cggcacggtg gcctcggcga
    16801 ggtcctcgct ggtcgcctcc tcgccgccga gcgtggccac gagccgggac acgacatgct
    16861 gggccgagac gttcgagtcg ccgacggctg cgaagaggct gtcgatgtcg gagtagcgca
    16921 ggtcgccggc gacggcggtc aggctctcgg cggacatcaa ccgatgcagc gggagaccct
    16981 gcttgcgcag gtacttggcg atcgcatcct tgccctgctc gatcgcctcc tcacgccgtt
    17041 ccttggtgaa ccactggcgg atcttgttgc gggcgcgagg gctcttgacg aacgcgagcc
    17101 agtcccgcga cggtccggcg ccgtcggcct tcgaggtgag gatctcgacc gagtcgccgt
    17161 tctccaggac cgagtccaaa ggggccagac gtccgttgac gcggccaccg atgcagtggt
    17221 gcccgacctc ggtgtgcacg gcatacgcaa aatcgacggg agtcgacccg gcggggagtg
    17281 agacgaccgc gcccttgggg gtgaagacga agacctcgcc gccgccgagg tcgaaacgca
    17341 gcgagtcgag gaactccccc gggtcggcga tctcgcgctg ccagtcgagc aggcggcgca
    17401 gccaggcagt ctcggcgagc gaatcggact cgacgggtgc gccgcggccg ccgcgcttgt
    17461 ggtcttcttt gtacttgtag tgcgccgcca cgccgtattc ggcccgtcgg tgcatcgtgt
    17521 gggtgcggat ctggatctcg accggcttgc cctgcggccc gatgaccgtg gtgtgcaggg
    17581 actggtacat gttgaacttc gggatcgcga tgtagtcctt gaaccggccc ggcaccgggt
    17641 tccaccgcgc gtgcagcgac ccgagcgcgc cgtaacagtc gcgcaccgtc tcgacgagca
    17701 cccgcacgcc gaccaggtcg tagatctcct cgaagtcgcg cccgcgcacg atcatcttct
    17761 ggtagacgga gtagtagtgc ttgggccgac cggtgacggt cgccttgatc ccggccacct
    17821 tgaggtcctc ggcgacctcc gaacagaccc tggcgaggaa ctcctcccgc gcgggggcgc
    17881 gagcggccac gaggtcgacg atctcgtcgt acatcttggg gtagagcgcc tggaaggcca
    17941 ggtcctccaa ctcccacttg atggtgttca tgcccagccg gtg
//
LOCUS       DAORPW010000037        17837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_584_length_17837_cov_785.633956, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000037 DAORPW010000000
VERSION     DAORPW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 17837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17837
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_584_length_17837_cov_785.633956"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /gene="thrS"
                     /locus_tag="PLB86_04930"
     CDS             complement(<1..437)
                     /gene="thrS"
                     /locus_tag="PLB86_04930"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927499.1"
                     /note="catalyzes the formation of threonyl-tRNA(Thr) from
                     threonine and tRNA(Thr); catalyzes a two-step reaction,
                     first charging a threonine molecule by linking its
                     carboxyl group to the alpha-phosphate of ATP, followed by
                     transfer of the aminoacyl-adenylate to its tRNA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRC64512.1"
                     /translation="MSASITVSVAGNERSVAEATTAADLFADDRAIVVARVNGELRDL
                     AHVLADGDAVEPVRIDDPDGLQVLRHSCAHVLAQAVQQVNPDAKLGIGPPIRDGFYYD
                     FDVTTPFTPEDLKELEKVMQRIINEGQTFVRREVSDEDALVELA"
     gene            complement(535..1446)
                     /locus_tag="PLB86_04935"
     CDS             complement(535..1446)
                     /locus_tag="PLB86_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRC64513.1"
                     /translation="MNLPPSGAAATGSVLLGGMLFGTAGVAQAFAPSGAHPLTVGAMR
                     LSVGAIALIIFVLARGDTFGSVVRLWGSPAGLIATAGAAAYQPFFFAGVARVGVPLGT
                     LVAVGSAPIFTGLLGWVWRRERPTGTWALATTICVAGLALLTGAGAQVGSLDGIALTA
                     GAGVSIACYTVAAKSLLESGVDAIGVCASTFLLGSIVVLPFALGQGIGWALTGSGVLV
                     ALYLGLATMAVANILYARGLLHLPPATAATLGLADPVTATALGVLVLGQTVSPLGWLG
                     FLGVVVGLLLQGIWAARAGRRDQLPLG"
     gene            complement(1453..1526)
                     /locus_tag="PLB86_04940"
     tRNA            complement(1453..1526)
                     /locus_tag="PLB86_04940"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1490..1492),aa:Val,seq:cac)
     gene            complement(1623..2996)
                     /locus_tag="PLB86_04945"
     CDS             complement(1623..2996)
                     /locus_tag="PLB86_04945"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRC64514.1"
                     /translation="MRLVLLGGGGFRVPLVHRALLDDEHAGRVDELVLHDVDEGRLET
                     MTRVLAQQGAGAPRPVTVRATARIDEALEGADFIFSAIRVGGLSGRTLDERIALDHAV
                     LGQETVGAGGISYGLRTVPVAVGIAERIAALAPNAWLINFTNPAGLITQAIQAVLGDR
                     AIGICDSPVGLGRRAATALGFQGADVQLGYAGLNHLGWVDSLVINGVDRLPDLLADDA
                     ALLSIEEGRLFGTEWIQSLAMLPNEYLYYYYFTREAVAAIMGEPQTRGEFLLDQQTGF
                     YQRALAAPSSAYRQWRSAWEDRETTYMREVRQPTVQRDARDFGGGYERVALALMAAIA
                     HDEPARLVLNVRNRGAVECLGVDAVVEVPCLVDAGGPVPQLSRISGGHERGLVQQVKA
                     AEELTIEAALTGSRRRAVQAFATHPLVDSVGVARDLFDAYAAAFPQLGAIFAAQPVPG
                     SPSVAGH"
     gene            complement(2993..3751)
                     /locus_tag="PLB86_04950"
     CDS             complement(2993..3751)
                     /locus_tag="PLB86_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRC64515.1"
                     /translation="MKYVRLRTITERLASGDSVSVLNLADELGVSPSTVRRDLQSLAA
                     SGILLRVHGGAAPVDGRDLGDRPFDLVADEDAIDKGRIAGRAATLVNDGDVVLLDIGT
                     TTRLLARELFGRPITAVTSSLAVLDELRGDTTSEVVLLGGAVRRQYHSLVGSLTEDAL
                     RQVRAATVFLSASGVSSDGAVLDTTLVEMPTKRGLLRAANRVVLLAGSSKFPGSGSFR
                     VCGMDQIDVLVTTHGADPATLKACRAQGTEVLFA"
     gene            3839..4957
                     /locus_tag="PLB86_04955"
     CDS             3839..4957
                     /locus_tag="PLB86_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRC64516.1"
                     /translation="MVVITEDFRGDDAPKDDRRRDDRVKDERGEAAPSLTLQSLSVDV
                     LVVGVTYLDVVVADLDEFPGPGEEARAGGLTLCPGGIATRAVATARLGLDTVLSTSLG
                     TDRAGALVRAALGQEAVRLDLADDLAGASTPVTVSVAHDGDRRMFTRDSLAPVRQPRM
                     LLDDGMCRCIMVDLRPGQDEILPLLVDVWTAGAYVVADVGWDPSGIWDPAVLKPLSVC
                     DLFTPNAVEAMAYTGADTPEDAARRLAEQVPLAVVTDGARGVHAASLEGTRPCEVFVP
                     AVTAYGTRQRDATGAGDVFGAALVAGTLWGLPLADRLAFGALCAALSLSSLGGAPGAP
                     GWTELQRWWSRALQQGDSPVLSRYEFLDSLLADQRCSA"
     gene            4954..7776
                     /locus_tag="PLB86_04960"
     CDS             4954..7776
                     /locus_tag="PLB86_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64517.1"
                     /translation="MTDDAEPITTVWLVPHTHWDREWYEPFQRFRLRLVDLMDDVVDR
                     ALAEPEFRFTMDGQMAAVDDYLEVRPERTEAIRRLVAHGQLAIGPWRVLMDEFLCSGE
                     TMIRNLEIGLADAARLGPSMPVGYLPDMFGHCAQMPQLLLGVGIERACVWRGVPAGVK
                     RSEFAWRSPDGSTIRTEYLANSYGNAADLFADTEGTAGRLLARIRTQSQWYAAGGFLA
                     MYGTDHAAPLPGLLDRVTALNASDGPEVRVATLTEYLDAIPAPVDSPVVPGELRSHAR
                     ANILPGVLSVRWPLKEAMAVSERLLARYAEPLAALVLREVPQGYLDLAWRRVVDASCH
                     DSVTGCGVDETALQVQARLEEAGHLAQAVRDRALDLLAQASPAGSVVVVNPLPTPRDD
                     LIELTLPVPSQWPAVELVSSTGQVVATQELSRPEPVLARETVASADLQRLIHRIHDRE
                     LFGLQLTGVDIDADERRVTFALAEAGDPGFDSVATGDRLAQAAARHTPQAPWEVVTRA
                     APTRTLAAKVPVPALGWASFQVRPGQAVAEPSNAASPLVPGGSVLPILCADRTLDNGL
                     IRVEVHDDGTFSVRGADGSFATGLGRIVDGGDCGDAYNYGPPGVDALVTTPLSVTVES
                     GGSRGPLLGRLTVTRDYALPATSDHGGRSAEVVATPVVMHLEVRAGEPFVRVAVSWEN
                     RTRDHRLRLHLPTPRTASGSHAQGQLAVVSRSRHPESGPVGEYPLATFPAERFIDAGG
                     LGAILTRTTEYEITDGPDGTEIALTLLRAVGWLSRNVHPHRNEPAGPQLPTPAAHVIG
                     PGSLNLAVMPHDGSWAQAGLGRWAESMLHPVVVLRGGASEGSETDSVEGLTIDGKEVD
                     LLSLRRREDGWLQVRVVNSCDEPDTLRLSSAVRPVLAAVRCDLLGRHGEVLDVIDEGA
                     VLVPVRPWEIATIALDVGPGPLLHKQ"
     gene            complement(7819..>8022)
                     /locus_tag="PLB86_04965"
     CDS             complement(7819..>8022)
                     /locus_tag="PLB86_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64518.1"
                     /translation="TAVSAGREGPLLLREGDEVVCFNADGLLLTGPGQPRRLIHGDLV
                     TIRCEPGPWWLEDGRRVVHALTH"
     assembly_gap    8023..8122
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8123..8777)
                     /locus_tag="PLB86_04970"
     CDS             complement(<8123..8777)
                     /locus_tag="PLB86_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRC64519.1"
                     /translation="MDVPRDVEALRIDLDVAANVGSVIDLGAQSPRGYVGWSGGARRQ
                     IVISAEWSTPGYLPTRGYAGTWQVLLGLHRVPADGARTTVTVRESNAGEVARLRALEP
                     ADPPVPLRPPRRTLPSSSGLTWLAADFHTHTVHSDGSLSVGGLAALAVSRGLDVLAIT
                     DHNTTSHHAELPAAATRYGIQLVPGQEITRDTGHANAFGDIGFVDFRTPPATWAAEVA
                     "
     gene            complement(8855..9961)
                     /locus_tag="PLB86_04975"
     CDS             complement(8855..9961)
                     /locus_tag="PLB86_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC64520.1"
                     /translation="MGEIRIEGLTKVYSGNVTALDRLDLTINDGEFFALLGPSGCGKT
                     TLLRTIAGLEVATSGRIHIGERDVTKMSPGDRDVAMVFQDYALFPHMTVSDNIAYPLR
                     IRKVDQASRAQTAARVGAGLGLNGLLERRPGELSGGQQQRVALARAMACEPSAFLFDE
                     PLSNLDARLRLEARTFLKGLQHQLGVTTVFVTHDQAEALAMADRIAVLEAGRLRQLGT
                     PAEIFHRPIDTFVANFIGSAPMTMLDADLVRGRLYVAGTTIEQEVAVRWSGPDPSDGP
                     VVLGIRPEYVRVGAFGPMTVQATIRIVENLGATVLLTAETASGDRLQALVPDGTQPAV
                     GAQVELSPVAERVLLYDEEGHLMTPATQAAPAPA"
     gene            complement(9961..10782)
                     /locus_tag="PLB86_04980"
     CDS             complement(9961..10782)
                     /locus_tag="PLB86_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRC64521.1"
                     /translation="MSPQAFLRRVGFVLFLAVVTALWALPMLWLVTAPFDAHPGLTVR
                     WPEWTLDNFRRLSESPYAFSSLRNSVLLAGGAMILVTVFGALAAYALSRVHVPGRGAI
                     LYGMLLLSSIVTGTAAMVPIFQLMFTLGLIDDRTGVILVLTGGLLPAAIFVLKDFMDA
                     TPRSYEESARVFGASPTQVLRHVVLPIARPGLATIAVWALVNAWGDFLTAYILLRDPT
                     KTPAGVVMYTFYSEGGQANLPLISAFSVLYALPVVVLYIFVSRRYGFRFHGGIKS"
     gene            complement(10779..11660)
                     /locus_tag="PLB86_04985"
     CDS             complement(10779..11660)
                     /locus_tag="PLB86_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRC64522.1"
                     /translation="MSDHAADAAGLGRPRAAAFMAPALLLITAFLVVPALWTLVLGAT
                     NYRLTGLAAANPQFVGTGNITEALSDPGFGWSLWLTLLFVLGSAIFGQSLAGLSLAWS
                     LRSAPKVVKAVVETLVLMAWILPSSVVAFLWVAYLSRHGGTLNAILQTPGTDWLIQHP
                     MASIIVFNIWRGTAFSMLLFSAALSAVPPSQMESARLAGAGTLAQLRDVVFPHIRGHV
                     LTNTLLISLWTFNDFTPYLLTAGGPNHATEILPVFIYNRALYGGELGYGSAISFLMLI
                     INLVIALFYVRLLRERR"
     gene            complement(11657..13090)
                     /locus_tag="PLB86_04990"
     CDS             complement(11657..13090)
                     /locus_tag="PLB86_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRC64523.1"
                     /translation="MTGAPLSSSPITRRSILIGTVGMAAAALASCDKASSSTSSTSAN
                     ATSRTVTLSIAANAISGGKSSKEADWFAKYVIPEFIKAQQAKGVTATVSFQPSGVDDE
                     QYKSKLALDLKSKSGADIVSLDGIWVGEFAQAGYIKPLKDVVGSSVTSWEGWSQIPEA
                     VQANAAFDGNTFGIPAGTDGRVLYFNKKLFAQAGLPANWQPTSWTDLISAGEKLATLS
                     GVTPLQIDAGTAMGEATTMQGFLPLLVGAGQQIYANGKWQGNTQAVRDVLTFYQTILA
                     KKLEDSTLQLAAKGRDESFGAFAQGKIGVLAEGDYFWRSVINPNGGDAPMATRDSDVG
                     WAFIPAMAPGKGVGGADYASMSGGGGYFLNPSTPYPQQAWELLTFMSSKAAILARLDG
                     AAQVTARKDVNSEVLSKDPLLSFVSQKVLPLTHYRPGLAVYPQVSLALQQATADVVSG
                     KSPADAATAYQAALVKALGGADQVTTS"
     assembly_gap    13485..13584
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13592..14284)
                     /locus_tag="PLB86_04995"
     CDS             complement(13592..14284)
                     /locus_tag="PLB86_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC64524.1"
                     /translation="MLEVKGVCAGYGRVQVLWDVDLTVSANEIVALVGPNGAGKTTLL
                     RALSGMVPIRDGSVTFLGEDITGRSIERIVDLGISHVPEGRRLFPGLTVKDNLRLGGW
                     RNRNTDIDRVIDLFPKLGDRLSQVTGSMSGGEQQMCAIARGLMGKPDLIMIDELSMGL
                     APVIVEEIIGRLADIAASGTAVLLVEQDVDAALTVAERAYVMETGRVVMSGNAGVLLA
                     DPRIQQSYLGIA"
     gene            complement(14277..15131)
                     /locus_tag="PLB86_05000"
     CDS             complement(14277..15131)
                     /locus_tag="PLB86_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC64525.1"
                     /translation="MSAVPVLETRGLGIRFGGLKAVDDVSLSVPEGLTLGVIGPNGAG
                     KSTFINLVTGHLKPTSGTVLVDGRDLTGVKPWVIAKARVARTFQIVKPFRGMTVRENV
                     AVGVLYGPDGTGRMSTALKQADEILAEVGIFELGDRPPGELSVADARRLEFAKALALK
                     PRLLLLDEVMAGLRPGEIEPSLQLIRDLKARGMTIIVVEHVMKAILAVSDEVLVLNAG
                     QRLMLGPPREVLSDPQVIEAYLGHKYATRAAQLSEAASAALAAPTPTPTPTPKAVDIA
                     EEEVGPDA"
     gene            complement(15128..16129)
                     /locus_tag="PLB86_05005"
     CDS             complement(15128..16129)
                     /locus_tag="PLB86_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC64526.1"
                     /translation="MATVTPALWRRWGPWALGLVGALAALGLQTQLYTGQVRTVATIF
                     MFVALALSWNLIGGYTGYASFGQVVFFGLGGYFAAVTMTHLHWSFWVALPLAGLAAAA
                     YAVVIGIPLLRLRGHYFAIATLGVAEGTREVVINLDGLTGGGGGITVPTVGAAAATKY
                     LGNDGFYLVYLALAVLALLITGLVSRSKFGFAMRAIHQDEDAAAAVGINTTRTKVLTF
                     ALSGLLTGLVGATYAFQQVTIYPERLFDVEITVLMVVMVVIGGSGTVLGPIIGAVALQ
                     FLSEWLRQNYTTVHTLLLGIIIIIAVILLPQGLVNYLREARRNGDYSLLANVRRYRL"
     gene            complement(16129..17013)
                     /locus_tag="PLB86_05010"
     CDS             complement(16129..17013)
                     /locus_tag="PLB86_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC64527.1"
                     /translation="MTGFLQATVYGLLQGGLLALVAVGFSLVWGVMNVVNLSHGAFVV
                     LGAYVGWQLNTSLGLDPFLGMVIVALVLFCIGYAVQRFLINLVVNGPIFITLLLTFGL
                     ELLLVQGMNIVFTANYQSIPTSYAGNAIVLGGIQVPLGRLLAFLLAVGITVLLVKVMA
                     NTRIGMSIMATGMDRGAARLMGIRARHVYALTFGIATALAGMAGTMVGTVGTFNPAAA
                     GGFTLLSFVIAVLGGLGNMYGAMVGGLLMGLVQAWGGYYLSGTWVNALAFLVLVIVLM
                     FRPSGLVGKAFYAARVEV"
     gene            complement(17010..>17837)
                     /locus_tag="PLB86_05015"
     CDS             complement(17010..>17837)
                     /locus_tag="PLB86_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC64528.1"
                     /translation="MVDSSGADNNIFAKGYKRTFGVLSPATEYAASIVKAVAELASPK
                     PTSVVVLSADDGFSKRAADGAAKAAKDLGFTVLETQYFPNGATDVSASLNKVKASNPD
                     VIIGSVHLAEGVAIIKQAKELGVVPKVFGETVAPPTPDFVTTLGKDAEGVLGSSQWTK
                     SAAGSDDYFGTAKQYDADIQAKFKHEAGYHNAEASAACLAFVLAIQKAGSTDANKVRD
                     ALAALDAPSFFAQLKFDAVGMNSTKPMSVIQIQGGKQVTVWPKTSAEAALIWPGTTK"
BASE COUNT         2906 a         5936 c         6127 g         2668 t
ORIGIN      
        1 gccagctcga ccagggcgtc ctcgtcgctc acctcgcggc gcacgaaggt ctggccctcg
       61 ttgatgatcc gctgcatgac cttttcgagc tctttgaggt cttcgggcgt gaacggcgtg
      121 gtcacgtcga agtcgtagta gaagccgtcc cggatgggcg ggccgatgcc gagtttggcg
      181 tcggggttga cctgctggac ggcctgcgcg agcacgtgcg cgcacgagtg acgcagcacc
      241 tgcagcccgt cggggtcgtc gatgcgcacc ggctcgacgg cgtcgccgtc ggccaggacg
      301 tgggccaggt cgcgaagctc accgttgacc cgggcaacaa cgatcgctcg gtcatcggcg
      361 aacaggtcgg cggcggtagt cgcctccgcg accgatcgct cgtttccggc gacggacacg
      421 gtgatgctgg cagacactgc gggctccctg cggtgagtgg cgcgggcagg ccccgcgcgg
      481 catacccaag gttatcgggc cttcgcgtcc acggtcgcgc cggtttcggc gcgctcagcc
      541 cagcgggagt tggtcgcgcc gcccggcgcg cgccgcccag atcccttgca gcaacagccc
      601 cacgacgact ccgaggaagc ccagccatcc caggggcgag accgtctgcc cgaggacgag
      661 gaccccgagt gcggtggcgg tgaccgggtc ggcgagcccc agagtcgcgg cggtggccgg
      721 gggcaggtgc agcaggccgc gggcgtagag gatgttcgcg accgccatgg tggccagccc
      781 gagatagaga gcgacgagga caccgctgcc ggtcagggcc cacccgatgc cttggccgag
      841 cgcgaacggc aggacgacga tgctgccaag caggaaggtc gaggcgcaca ctccgatggc
      901 atccacaccc gactccagga gcgacttcgc cgcgaccgtg tagcaggcga tcgacactcc
      961 tgctcctgcg gtcaaggcga tcccgtcgag gctcccgacc tgggcacccg cgccggtcag
     1021 gagcgccagt ccggccacgc agatggtggt ggccagcgcc caggtgcccg ttgggcgctc
     1081 gcgccgccag acccagccga gtagcccggt gaagatggga gcgctcccca cggcgaccaa
     1141 cgtgcccagc ggcacgccga cccgcgccac cccggcgaag aagaacggtt ggtatgccgc
     1201 ggccccggcc gtcgcgatca gcccggctgg gctcccccac agccggacga cggaaccgaa
     1261 ggtgtcacca cgagccagga cgaagatgat gagggcgatc gcgccgacgg acagccgcat
     1321 ggctccgacg gtgagcgggt gggcaccaga gggggcgaag gcttgggcga cgccggccgt
     1381 gccgaacaac atcccgccca gcaggacgga tccggttgcg gcagcgcccg atggggggag
     1441 attcaccaag ggtggatgtg aagggactcg aacccctgac ctctcgcgtg tgaagcgaac
     1501 gctctaacca actgagctac acatccagat gcggccaggg tcgccggccg cgagtggcaa
     1561 ggatagcggg cgggtccggt cgagtgccaa tcaccacccc gaccggggta tgccgcctcg
     1621 cctcagtgac ccgcgacgga gggtgaccca gggacgggtt gagcggcgaa gatggcaccc
     1681 aactgcggaa aggctgcggc gtaggcgtcg aagaggtccc gggcgacccc gactgagtcg
     1741 acgagcggat gggtggcgaa cgcttggacc gcgcggcgcc tggacccggt gagggccgcc
     1801 tcgatcgtca actcctccgc cgccttgacc tgttggacga gtccgcgctc gtggccaccg
     1861 ctgatccgac tgagctgggg caccgggccg ccggcatcca ccagacaggg cacttccacg
     1921 acggcgtcga ccccgaggca ctccacggcg ccacgattcc tcacgttgag gaccagtcga
     1981 gcgggttcgt cgtgcgcgat cgcggccatc agcgcgaggg cgacccgttc gtagcctccc
     2041 ccgaagtccc gcgcgtcccg ctggacggtg ggctggcgca cctcacgcat gtaggtcgtc
     2101 tcgcggtcct cccaggccga gcgccattgg cggtacgcgc tggaaggggc cgcgagggca
     2161 cgctggtaga acccggtctg ctgatcgagg aggaactcgc cccgagtctg tggctcgccc
     2221 atgatggccg cgacggcctc tcgggtgaag tagtagtagt agaggtactc gttgggcagc
     2281 atggccaggg actggatcca ctcggtgccg aacagccgac cctcctcgat cgacagcagg
     2341 gcggcgtcgt cggcgaggag gtccgggagt cggtcgacgc cgttgatgac gagcgagtcc
     2401 acccacccca ggtggttcag accggcatac cccagttgga cgtcggcacc ctggaaaccc
     2461 agggctgtgg cggcgcggcg gccgaggccg accggcgagt cgcagatccc gatcgcgcga
     2521 tcaccgagaa cggcttggat cgcctgggtg atgaggcccg cagggttggt gaagttgatg
     2581 agccaggcgt tcggcgcgag agccgcgatc cgctcggcga tgcccaccgc caccggcacc
     2641 gttcgcagtc cataactgat gccgccggcg ccgaccgtct cctggcccag cactgcgtga
     2701 tcgagggcga tccgctcgtc gagcgtccgg ccgctcagcc cgccgacccg gatggccgag
     2761 aagatgaagt cagcgccttc gagggcctcg tcgatgcggg cagtggcgcg gacggtcact
     2821 ggcctaggcg cgccggctcc ctgctgagcg agcaccctgg tcatcgtctc gaggcgcccc
     2881 tcgtcgacgt cgtgcaggac gagttcgtcc acccgcccgg cgtgttcgtc gtccaacagc
     2941 gcccggtgca ccagggggac cctgaatcca ccgccgccga ggaggacgag cctcatgcga
     3001 agaggacctc cgtcccctgg gctcgacagg ccttcagcgt cgcgggatcg gctccgtggg
     3061 tggtgacgag gacgtcgatc tgatccatcc cgcacacgcg gaaggaaccg gatccgggga
     3121 acttgctgga cccagccagc aggacgaccc ggttggccgc acggaggagg ccgcgcttgg
     3181 tcggcatctc gaccaacgtg gtgtccagca ccgccccgtc cgaactgacg ccgctcgcgc
     3241 tgaggaacac cgtggcggca cggacctggc ggagggcgtc ttcggtaagg ctgcccacca
     3301 gcgagtggta ttggcgccgg accgcgccgc ccagcaggac gacctccgac gtcgtgtcac
     3361 ctcgaagttc gtcgagaacg gccagactcg acgtgaccgc ggtgatcggt cgcccgaaga
     3421 gctcacgggc cagcagccgg gtcgtcgtgc cgatgtccag cagcaccacg tctccgtcgt
     3481 tgacgagcgt ggccgcccga ccagcgatcc gccccttgtc gatggcgtcc tcgtcggcga
     3541 ccaggtcgaa cgggcggtcg cccaggtcac gaccatcgac gggagccgca ccaccatgga
     3601 cccgcaggag gatgcccgag gccgccaagg actgcagatc gcgccgcacc gtggaggggc
     3661 tgacccccaa ttcgtcagcg aggttgagga ccgagacgct gtcacccgat gccagccgtt
     3721 cggtgatcgt gcgcagtcgc acgtatttca tcggtccgct cccatagcca tactgtagtg
     3781 aagtgcgcaa taatacgcaa taacgcgcga ctggttccac cgtgagaggg ccccgaagat
     3841 ggtcgtgata accgaggact tccgaggcga cgacgccccc aaggacgacc gccgacgaga
     3901 cgaccgagtc aaagacgagc ggggcgaggc tgctccctcg ctgacgctcc agagcctgtc
     3961 ggtcgacgtc ctggtcgtcg gggtgaccta cctcgacgtc gtcgtcgccg acctcgacga
     4021 gttccccggc ccgggcgagg aggcccgggc cggcggcctc acgctgtgtc cgggagggat
     4081 cgcgacccgg gcggtcgcca ccgcccgcct cgggctggac accgtcctat ccacttccct
     4141 cggcaccgac cgcgcggggg ccctcgtgcg ggccgccctc ggtcaggagg ccgtgcgcct
     4201 tgacctcgcc gacgacctgg cgggagcgag cactccggtc accgtctccg tcgctcacga
     4261 cggtgatcgt cggatgttca ctagggactc cctcgctccc gtcaggcaac ctcggatgct
     4321 gctcgacgac gggatgtgcc gctgcatcat ggttgacctc cgccccggtc aggacgagat
     4381 cctcccgctg ctggtcgacg tctggactgc cggggcctac gtcgtagccg acgtcggatg
     4441 ggatccttcg ggcatctggg atccggcggt gctcaaaccc ttgagcgtct gcgatctctt
     4501 cacccccaac gccgttgagg cgatggccta caccggcgcg gacacccccg aagacgcggc
     4561 gcggcggctc gccgagcagg tccccctcgc cgtcgtcacg gacggggccc ggggcgtgca
     4621 cgccgcgtcg ctggaaggca cccggccgtg cgaggtgttc gtgccggctg tcacggcata
     4681 cgggacgcgc caacgcgacg ccacgggagc cggcgacgtg ttcggcgccg cgctggtggc
     4741 agggacgttg tggggtctcc ctctggctga ccggctggcg ttcggggccc tctgcgccgc
     4801 cctgtcgctg tcctccctcg gtggtgcgcc gggggctccg gggtggacgg agctccagcg
     4861 ctggtggtcg cgcgctctgc aacagggaga ctcccccgtg ctctcccgct acgagttcct
     4921 cgacagcctt ctggccgacc agagatgctc cgcgtgacgg acgacgcgga gccgatcacg
     4981 acggtctggc tcgtccccca cacccactgg gaccgcgagt ggtacgagcc cttccagcga
     5041 ttccgcctcc gtctggtcga cctcatggac gacgtcgttg accgagccct cgccgagccc
     5101 gagttccggt tcacgatgga cggacagatg gcggccgtcg acgactacct cgaggtccga
     5161 ccggagcgga ccgaggcgat tcgtcgcctg gtcgcccatg gtcagttggc gatcggcccc
     5221 tggcgggtcc tcatggacga gttcctctgc tcgggcgaga ccatgatccg aaaccttgag
     5281 atcgggctcg ccgacgctgc gcgcctcggc ccgagcatgc ctgtgggata tctcccggac
     5341 atgttcgggc actgcgccca gatgccccaa ctgctgctcg gcgtgggcat cgagcgcgcc
     5401 tgcgtctggc gcggcgtccc ggctggtgtc aagcgcagcg agttcgcctg gcgctccccc
     5461 gacggctcga ccattcgcac cgaataccta gccaacagct acggaaacgc cgcagacctc
     5521 ttcgccgaca ccgaggggac cgcgggtcgg ctcctcgcca ggatcaggac ccaatcgcag
     5581 tggtatgccg cgggcggctt cctcgcgatg tacggcaccg accatgccgc accactccca
     5641 ggcctgctcg accgggtgac ggcgctcaac gcgtcggacg ggccggaggt ccgggtcgcc
     5701 accctcaccg aatacctcga cgccatcccc gcgccggtcg actcacccgt cgtccctggt
     5761 gagttgcgca gtcacgcccg tgccaacatc ctgcccggcg tcctctccgt gcggtggcca
     5821 ctcaaggagg ccatggcggt gagcgagcga ctgcttgccc ggtatgccga gccgctggcg
     5881 gccttggtgc ttcgggaggt cccccagggt tacctcgatc tggcctggcg acgggtggtc
     5941 gatgcgtcgt gccatgactc ggtcaccggt tgcggggtcg acgagaccgc ccttcaggtg
     6001 caggcccgac tcgaggaggc cgggcacctc gcccaggccg ttcgcgaccg cgccctggac
     6061 ctgctggccc aggccagccc cgcgggctcc gtcgtcgtcg tcaacccgct gccgacgcct
     6121 cgggatgacc tgatcgagct gaccttgccg gttccttcgc agtggcccgc agtggagttg
     6181 gtctcgtcga cgggccaggt cgtggccacc caggagttgt ctcggccgga gccggtgctg
     6241 gcgcgagaga ccgtcgcatc ggcggacctg cagcgcctga tccaccgcat ccacgatcgt
     6301 gaactgttcg gcctgcaact gacgggtgtc gacatcgacg ccgacgagcg acgggtgacg
     6361 ttcgcgctcg cggaagccgg agacccaggg ttcgacagcg tcgccacggg cgaccgactc
     6421 gcacaggcgg ctgcgcggca tactccgcag gcgccgtggg aggtcgtcac tcgggccgca
     6481 cctacgcgga ccctggccgc gaaagtcccc gtgccggcgc tgggttgggc gagcttccag
     6541 gtccggccgg gccaggccgt ggccgaacca tccaacgcgg ccagtccgct ggtgccgggc
     6601 gggtcggttc tccccatcct gtgcgcggac cggaccctgg acaacgggct catccgggtc
     6661 gaggtccatg acgacgggac gttctccgtg cgcggtgcgg acgggtcgtt cgcgacgggc
     6721 ctgggccgga tcgtcgacgg tggggactgc ggcgacgcct acaactacgg gccgccgggc
     6781 gtcgatgcac tcgtgaccac tcccctgtcg gtgaccgtcg agtcgggagg gtcccgcggg
     6841 ccgttgcttg gacgcctgac ggtcacgcgc gattacgccc tgccggcgac gagtgatcat
     6901 ggcggtcgtt ccgccgaggt ggtcgccacc cctgtcgtca tgcacctcga ggtgagggcg
     6961 ggcgagccgt tcgtgcgggt ggcggtgtcc tgggagaacc ggacccgtga ccaccgcctg
     7021 cggctgcacc tgccgacgcc gcgcaccgca tcagggtcac acgcccaggg ccaactcgcg
     7081 gtggtctccc gcagtcggca tccggaatcc ggtccggtcg gcgagtaccc gctggccacc
     7141 ttccctgccg agcggttcat cgatgccggg ggcctcggag ccatcctcac gcggaccacc
     7201 gaatacgaga tcactgacgg gccggacggc accgaaatcg cgctaaccct cctgcgcgct
     7261 gttggctggt tgagcagaaa cgtgcatccc caccgcaacg agccggcggg gccgcaactg
     7321 ccgacaccgg ccgctcatgt catcggccct gggtcgctga acctcgctgt catgccgcac
     7381 gacgggtcct gggctcaggc cgggctcggc cgatgggccg agtcgatgct ccacccggtt
     7441 gtcgtccttc gggggggcgc ttccgaaggg tcggagacgg actcggtcga gggtctgacg
     7501 attgatggga aggaggtgga cctgctctcg ctgcgccgcc gggaggacgg ctggctgcag
     7561 gttcgggtcg tcaactcgtg cgacgaaccc gacacactgc ggctctcatc cgccgtccgc
     7621 ccggtgctcg cggccgtccg atgcgacctg ctcggccggc acggtgaggt gctcgacgtc
     7681 atcgacgagg gtgccgtcct cgtgccggtg cggccctggg agatcgccac gatcgccctc
     7741 gacgtcggtc ccggacccct tcttcacaag cagtgacggt cgaggtgtct gcagtgccgg
     7801 tgacccggag agtccgcgtc agtgggtcag agcgtgcacg acgcggcggc catcctcgag
     7861 ccaccatggc cccggctcgc accggatcgt caccagatca ccatggatca gccgccgggg
     7921 ctgcccaggc cccgtgagta ggaggccgtc cgcgttgaaa cagaccacct cgtcgccctc
     7981 tcgcaacagc agtggcccct cccgtccggc gctgacggcc gtnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nntggcgacc tcagccgccc acgtcgccgg cggcgtgcgg
     8161 aagtcgacga agccgatgtc gccgaaggcg ttggcgtgcc ccgtgtcgcg ggtgatctcc
     8221 tgcccaggga ccaactggat cccgtatcgc gtggctgccg ctgggagttc ggcgtggtgg
     8281 ctggtggtgt tgtggtcggt gatggccaag acgtccagac cccggctcac cgcgagagcg
     8341 gccagccctc cgaccgagag gctgccgtcg gagtggacgg tgtgggtgtg gaagtcggcc
     8401 gccaaccacg tcaacccact cgaggacggc aacgtgcggc gaggcggacg cagtggcaca
     8461 ggagggtccg caggctccag agcccgcaac cgcgccacct cgccggcgtt ggactccctc
     8521 accgtgaccg tcgttcgtgc tccgtcggca ggcacccggt gcagcccgag gagaacctgc
     8581 caggtgccgg catacccacg tgttggcagg taaccgggcg tcgaccactc ggcgctgatg
     8641 acgatctggc gccgtgcgcc acccgaccag ccgacatacc cgcggggaga ctgcgccccg
     8701 aggtcgatga ctgatccgac gttggctgcg acgtcgaggt cgatccgcag ggcctccacg
     8761 tcgcggggga cgtccacggg cagagaccga atctgaagtt cggcccgatc tgctggagtc
     8821 caggtgtgct tgtgaacctg caggacgcgg ccgctcacgc tggcgccggg gcggcttgcg
     8881 tggcaggggt catcaggtgg ccctcctcgt cgtagagcag cacccgttcg gcgaccgggc
     8941 tgagctcgac ctgggcgccg acggctggct gggtgccatc ggggaccaag gcctgcagcc
     9001 ggtcccctga cgccgtttca gcggtgagca ggacggtggc accgaggttc tccacgatgc
     9061 ggatggtcgc ctgcaccgtc atcggtccga aggcacccac ccggacgtac tcaggccgga
     9121 tgcccagcac gaccggaccg tcgctcgggt ccggcccgga ccaccgcaca gcgacttcct
     9181 gctcgatcgt cgtccccgcg acgtacagtc gaccgcggac gaggtcggca tcgagcatcg
     9241 tcatcggcgc ggagccgatg aagttcgcga cgaacgtgtc gatcgggcgg tggaagatct
     9301 cggccggcgt gccgagttgg cgcagccgcc cagcctccag gacggcgatc cggtcggcca
     9361 tggccaaggc ttcggcctga tcgtgggtga cgaacaccgt ggtgacgccg agttggtgct
     9421 gcagcccctt gaggaaggtc cgcgcctcca gccgcagtcg ggcatcgagg ttgctcagtg
     9481 gctcgtcgaa caggaacgcc gacggctcgc aggccatcgc ccgggcgagt gcgacgcgtt
     9541 gctgctggcc tccggagagc tcccccggcc gtcgctccag cagcccgttg agcccgagcc
     9601 cagcccccac ccgggcggcg gtctgtgcgc ggctggcctg gtcgaccttg cggatgcgca
     9661 gggggtaggc gatgttgtca ctgaccgtca tgtgcgggaa gagggcgtag tcctggaaga
     9721 ccatggccac gtcgcggtcc cccggcgaca tcttcgtgac gtcgcgctcg ccgatgtgga
     9781 tccgaccgct cgtcgcgacc tcgagcccgg cgatcgtgcg caacagcgtg gtcttgccgc
     9841 aaccggacgg accgagcagc gcgaagaact caccatcgtt gatggtcagg tcgagccggt
     9901 ccaacgccgt gacgttcccg gagtagacct tggtcagccc ctcgatgcga atctcgccca
     9961 tcagctcttg atccctccgt ggaagcggaa cccgtatcga cggctgacga agatgtagag
    10021 aacgacgacc gggagtgcgt aaagcaccga gaaggccgag atgagaggca agttcgcctg
    10081 tcctccttcg ctgtagaagg tgtacatcac gacgcccgcc ggagtcttcg tcgggtcgcg
    10141 caagaggatg tatgccgtga ggaagtcgcc ccacgcattg accagggccc acaccgcgat
    10201 cgtcgccaga ccagggcggg cgatgggaag gacgacgtgg cgcaggacct gggtcgggct
    10261 ggcaccgaaa acccgcgcgc tctcctcgta ggaccgtgga gtggcgtcca tgaagtcctt
    10321 aaggacgaag atggccgccg ggagcagccc accggtgagc acgaggatga cgccggtccg
    10381 gtcgtcgatc agcccgaggg tgaacatcag ctggaagatc ggcaccatcg ccgcggtgcc
    10441 ggtgacgatc gagctgagca gcagcatccc gtagaggatg gcgccccgcc caggcacgtg
    10501 aacgcgggag agcgcgtagg cggccaacgc accgaagacc gtcacgagga tcatggcgcc
    10561 cccggccaac aggaccgagt tgcgcagcga tgagaaggcg taagggctct cggacaggcg
    10621 acggaaattg tcgagggtcc actcgggcca ccggaccgtg agcccagggt gcgcgtcgaa
    10681 gggtgcggtg actagccaga gcatcggcag cgcccagagg gcggtgacga cggcgaggaa
    10741 gaggacgaaa cctacccgtc gcaggaaggc ctgggggctc atcggcgctc ccgcagcagc
    10801 cggacataga agagcgcgat gacgaggttg atgatcagca tgaggaacga gatcgccgag
    10861 ccgtagccca actccccgcc atagagggcc cggttataga tgaagacggg gaggatctcg
    10921 gtggcgtggt tgggacctcc tgcggtgagg aggtagggag tgaagtcgtt gaacgtccag
    10981 aggctgatga gcagggtgtt cgtcaggacg tgaccgcgga tgtgcggaaa caccacatca
    11041 cgcagctggg ccaacgtccc tgcgccggcc aaccgcgcgg attccatttg agacggtggc
    11101 acggcgctca gagccgccga gaagagcagc atggagaacg ccgtcccacg ccaaatgttg
    11161 aagacgatga tcgaggccat ggggtgctgg atgagccaat cggtccccgg ggtctgcagg
    11221 atggcgttga gggtgccgcc gtggcgggag aggtaggcga cccagagaaa cgcgaccacc
    11281 gaactcggca ggatccaggc catgaggacg agcgtctcca cgactgcctt gaccaccttc
    11341 ggcgccgacc gcagcgacca cgcgagagac agccccgcca gcgattgccc aaagatcgcc
    11401 gagccgagca cgaacaggag cgtgagccac agcgaccacc cgaagcccgg gtcggagagc
    11461 gcctcggtga tgttgcccgt gccgacgaac tgcgggtttg ccgcagccag ccccgtgagc
    11521 cggtagttcg tcgcccccag gacgagcgtc catagggcgg ggacgacgag gaacgccgtg
    11581 atgagcaaca gcgctggagc catgaacgcg gcggcccgcg gtcgaccgag gccggccgcg
    11641 tccgccgcat gatcactcac gaggtggtga cctggtcagc tcccccgagt gccttgacca
    11701 gcgcagcctg gtatgccgtg gcagcatccg caggagactt gccggacacg acgtcagcgg
    11761 tggcctgctg gagggcgagc gacacctgcg gatagacggc cagaccgggc cgatagtgcg
    11821 tgaggggcag caccttctgg ctgacaaaac tcagcagcgg atccttggag agcacctcgg
    11881 agttgacgtc cttgcgggca gtcacctggg ccgcgccgtc cagccgggcg aggatggcgg
    11941 ccttggagct catgaaggtc agcagttccc aggcttgttg cgggtagggc gtgctcgggt
    12001 tgaggaagta gccgccgccc ccagacatgg acgcgtagtc agctcccccg acacccttgc
    12061 cgggagccat cgcaggaatg aacgcccacc cgacatcgga gtcgcgggtg gccatcggcg
    12121 catccccgcc gttcgggttg atcaccgacc gccagaaata gtcgccctcg gcaaggacgc
    12181 cgatcttgcc ctgggcgaag gcaccgaacg actcgtcgcg ccccttggcg gccagttgga
    12241 gggtggagtc ctcaagcttc ttggccagaa tcgtctgata gaaggtgagc acgtcgcgga
    12301 cggcctgggt gttgccctgc cacttgccgt tggcgtagat ctgctgaccg gcaccgacga
    12361 gcagcgggag gaagccctgc atcgtcgtgg cctcacccat cgccgttccc gcatcgatct
    12421 gcagtggcgt gactccgctg agggtcgcga gcttctcgcc cgccgagatg aggtcggtcc
    12481 acgaggtcgg ctgccagttg gccgggaggc cggcctgcgc gaacaacttc ttgttgaaat
    12541 agaggacccg gccgtctgtc ccggccggga tcccgaaggt gttgccgtcg aaggcggcgt
    12601 tcgcctgtac cgcctccgga atctgcgacc agccctccca cgacgtcacg gacgagccga
    12661 ccacatcctt gagcggcttg atgtaccccg cctgggcgaa ctccccgacc cagatcccgt
    12721 cgagggacac gatgtcggcc ccggacttgc tcttgaggtc gagggcgagt ttcgacttgt
    12781 actgctcgtc gtccaccccg gagggctgga agctcaccgt cgcggtgacc cccttggcct
    12841 gctgcgcctt gatgaactca ggaatgacgt acttcgcgaa ccagtccgcc tctttcgaac
    12901 tcttgccgcc gctgatcgcg ttggcggcga tcgacagggt caccgtccgc gaggtggcgt
    12961 tcgcgctggt gctgctcgtg cttgaggacg ccttgtcgca cgaggccagg gcggccgcgg
    13021 ccatgcccac ggttccgatg agaatgctgc gtcgggttat cggggaagag ctcagtggtg
    13081 cgccggtcac gttctctcct tcgggtgagg ttcggtcaca catcccgggg tgcggggaca
    13141 ccatgaagtc ccgttgcgcc agccaggggc ggggcaaggc gcggtcccgt acgagggcgc
    13201 tggaagcggt ccaaagcgcc ccgacggcta ctccgtcggc gcggtgggtt cagccgcgac
    13261 gggcgggtgc aaccccggga tgtgtgcacc cccatactgt gacacatatc aaccagaacc
    13321 gcgcagcaat gcgcaacttg tctcgcgttg acccgtgggt aaacgcggga agtctgaggc
    13381 ggatgatgct gttttcaacc tcttcgcggc cccttgcaag cgcgcaaaac cgcgcaagac
    13441 gcgatccggt ggcgccacgg catacccaga cagaatcgcg ccgcnnnnnn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnncgcccc ctcaggcgat gccgagatag ctctgttgga
    13621 tccgcgggtc cgccagcagc acaccggcgt tccccgacat cacaactcga ccggtctcca
    13681 tgacataggc gcgttcggcc accgtgagag cagcgtcgac atcctgctcg acgagaagca
    13741 ccgcggtgcc cgacgcggcg atgtcggcga gccgcccgat gatctcctcg acgatgaccg
    13801 gggccagacc catcgacaac tcgtcgatca tgatgaggtc gggcttgccc atgaggccgc
    13861 gggcgatggc gcacatctgc tgctcgccac cggacatgct tcccgtcacc tggctgagcc
    13921 ggtcgcccag tttggggaac aggtcgatga cccggtcgat gtcggtgttg cgattgcgcc
    13981 atcctccgag gcggaggttg tccttgacgg tcaggcccgg gaagagccgc cggccctcgg
    14041 gcacgtggga gatcccgagg tcgacgatcc gctcgatcga ccttccggtg atgtcctcac
    14101 cgaggaaggt caccgagccg tcccggatcg gcaccatccc ggacagggcc cgcagcagcg
    14161 tcgtcttgcc ggcgccgttg gggccgacga gggcgacgat ctcgttggcc gacaccgtca
    14221 ggtcgacgtc ccacaacacc tggacccgcc catagccggc gcagaccccc ttgacctcaa
    14281 gcatccggac ccacctcctc ctcggcgatg tcgaccgctt tcggcgttgg cgttggcgtt
    14341 ggcgttggcg ccgccagcgc cgccgaagcg gcctcggaca gttgggctgc tcgggtggcg
    14401 tacttgtggc cgaggtaggc ctcgatcacc tgtgggtccg agagcacttc gcgcggtgga
    14461 ccgagcatga gtcgctgccc ggcgttgagc acgagcacct cgtcgctgac ggcgaggatc
    14521 gccttcatca cgtgctcgac gacgatgatc gtcatgccgc gggccttgag atcgcggatg
    14581 agttgcagcg agggctcgat ctcgccgggg cgcagcccag ccatgacctc gtcgaggagc
    14641 agcaaccgcg gcttgagggc caacgccttc gcgaactcca gccgtcgggc gtcggcgacc
    14701 gacaactcgc ccggaggccg atccccgagc tcgaagatgc cgacctcggc gaggatctcg
    14761 tcggcctgct tgagcgccgt cgacatccgc ccggttccat cgggtccgta gaggaccccg
    14821 accgcgacgt tctcgcggac cgtcatcccg cggaagggct tgacgatctg gaaggtccgt
    14881 gcgacccggg ccttggcgat gacccacggc ttgacgccgg tgaggtcacg accgtcgacc
    14941 aggacggtcc ccgacgtggg cttgaggtgc ccggtgacga ggttgatgaa ggtgctcttg
    15001 ccggccccgt tggggccgat gacaccgagg gtgaggccct cggggacgct gaggctcacg
    15061 tcgtcgacgg ccttgaggcc gccgaaacga atccccaggc cccgggtctc caggacgggc
    15121 accgcgctca cagccggtac ctcctcacgt tggccagcaa cgagtagtcc ccgttacgcc
    15181 gggcttcgcg caggtagttg accaacccct gcgggagcag gatcaccgcg atgatgatga
    15241 tgattccgag cagcagggtg tggacggtcg tgtagttctg ccgcagccac tcggagagga
    15301 actgcagcgc gacggcgccg atgatcgggc cgaggaccgt tccgctgcca ccgatgacga
    15361 ccatgacgac catgaggacc gtgatctcga cgtcgaagag gcgttcggga tagatcgtca
    15421 cctgttggaa ggcgtaggtg gcaccgacca ggccggtcag caaccccgac agggcgaagg
    15481 tgagcacctt cgtccgggtc gtgttgatgc cgacggccgc cgcggcgtcc tcgtcctggt
    15541 ggatggcgcg catggcgaaa ccgaacttgc tgcgcgacac caaccccgtg atgagcaggg
    15601 cgagcaccgc cagagcgagg tagacgagat agaacccgtc gttgccgagg tacttggtcg
    15661 ctgcggccgc gccgaccgtc ggcaccgtga tgccgcctcc cccgccggtc agcccgtcga
    15721 ggttgatgac gacttcgcgg gtgccctcgg cgaccccgag ggtggcgatc gcgaagtagt
    15781 gcccacgcag gcgcagtagc gggataccga tgacgacggc gtatgccgcg gcggcgagtc
    15841 ccgcgagggg cagcgccacc cagaagctcc agtgcaggtg ggtcatcgtc accgcggcga
    15901 agtagccgcc gagcccgaag aacaccacct gtccgaagga ggcgtagccg gtgtagccac
    15961 caatgaggtt ccaggagagc gcgagtgcga cgaacatgaa gatcgtcgcc accgtgcgga
    16021 cctggccggt gtagagctgg gtctgcagcc cgagggccgc caacgcaccg accaagccca
    16081 gggcccacgg cccccagcgg cgccagaggg ccggcgtcac ggtggccatc agacctccac
    16141 tcgggctgcg tagaacgcct tcccgaccag gccggacggg cggaacatga ggacgatgac
    16201 caggacgagg aaggccaacg cgttgaccca ggtgccggag aggtagtaac cgccccaggc
    16261 ctggaccagg cccatcagca gaccgcccac catcgcgccg tacatgttgc ccaggccgcc
    16321 gagcacggcg atgacgaacg acagcaacgt gaagccgccc gccgcagccg ggttgaaggt
    16381 gccgaccgtg cccaccatgg tgccggccat cccggccagc gccgtggcga tcccaaaggt
    16441 cagggcatag acgtgccgcg cccggatccc catcagccgt gcggcgccgc ggtccatccc
    16501 cgtggccatg atcgacatgc cgatccgggt gttggccatc accttcacca acaggacggt
    16561 gatcccgacg gcgaggagga aggcgagcag tcggccgagc ggcacctgta tgccgcccag
    16621 cacgatcgcg ttaccggcat acgaggtcgg gatgctctgg tagttcgcgg tgaagacgat
    16681 gttcatcccc tgcacgagca gcagttccaa cccgaaggtg agcagcaggg tgatgaagat
    16741 cggcccgttg acgacgaggt tgatgaggaa gcgctgcacg gcatacccga tgcagaacag
    16801 cacgagggcg acgatcacca tccccaggaa ggggtcaagc cccaaggaag tgttgagttg
    16861 ccacccgaca taggcgccga ggacgacgaa cgctccgtgc gagaggttga cgacgttcat
    16921 caccccccag accagggaga agccgacggc cacgagggcg agcagacctc cctgcaacaa
    16981 cccgtagacg gtcgcttgga ggaaaccggt cacttggtcg ttcccggcca gatcagtgcg
    17041 gcttcggcgg aggtcttggg ccacacggtg acctgcttgc cgccctggat ctggatgacg
    17101 ctcatcggct tggtggagtt catgccgacc gcgtcgaact tcagttgggc gaagaacgac
    17161 ggggcgtcca gagcggccag ggcgtcgcgc accttgttgg cgtcggtcga gccggccttc
    17221 tggatggcca ggacgaaggc caggcacgcg gcgctggctt cggcgttgtg gtagcccgcc
    17281 tcgtgcttga acttggcttg gatgtcggcg tcgtactgct tagcggtgcc gaagtagtca
    17341 tccgagccgg cggccgactt ggtccactgg ctcgacccga ggactccctc agcgtccttc
    17401 cccagggtgg tgacgaagtc gggcgtgggc ggtgcgacgg tctcgccgaa caccttcggc
    17461 accacaccga gttccttggc ctgcttgatg atggcgacgc cttcggccag gtgcaccgag
    17521 ccgatgatga cgtccgggtt cgacgccttc accttgttga gcgacgcgct cacgtcggtg
    17581 gcgccattgg ggaagtactg ggtctccagc acggtgaacc cgaggtcctt ggcggccttg
    17641 gcggcgccgt cagcggcacg cttggagaag ccgtcgtcgg ccgaaaggac cacgaccgag
    17701 gtcggctttg ggctggccag ttcggcgacc gccttgacga tcgacgcggc atactccgtg
    17761 gccggcgaga gcaccccgaa ggtgcgcttg tagcccttgg cgaagatgtt gttgtcggcg
    17821 ccgctggagt cgaccat
//
LOCUS       DAORPW010000038        17769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_588_length_17769_cov_773.914305, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000038 DAORPW010000000
VERSION     DAORPW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 17769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17769
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_588_length_17769_cov_773.914305"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..899
                     /gene="lexA"
                     /locus_tag="PLB86_05020"
     CDS             159..899
                     /gene="lexA"
                     /locus_tag="PLB86_05020"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRC64529.1"
                     /translation="MAEVTELPEREGDGLTVRQRRVLEVIRNSVDRRGYPPSLREIGE
                     AVGLTSPSSVAHQLAALERKGYLRKDPNRPRALEVVSPDAKASDRGYRGSSGMPPSSA
                     DAWEHGEAHDTETDSGDARPEASYVPVVGRIAAGGPILAEEAIEDVFPLPRQIVGEGQ
                     LFLLKVVGDSMVDAAICDGDWVVVRQQPTADNGDIVAAMIDGEATVKTFQRKAGRVLL
                     IPHNAAYEPIDGRDASILGKVTAVLRRI"
     gene            937..1746
                     /locus_tag="PLB86_05025"
     CDS             937..1746
                     /locus_tag="PLB86_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HRC64530.1"
                     /translation="MTTRLPFASYLDHLRIESEAFAVALTDVPDDRQVPTCPDWTALD
                     LAHHLTEVQSFWAAVVSGPLLTDEAAEDVAKPARATSVPAQLEDLRAASARLAAALAT
                     TPADTPAWTWSTEQTVGFTFRRQAHEALVHRLDAQLTAGLRTPVDPTLATDGVDEALR
                     VMFGGCPPWGQITPVAGPEVRLATSDTGVSWLISPARFTGTDPDGTAYDDPDIVVAES
                     DSGGDVAATITGTAEDLLCWLWKRPTVGTLSHTGDESALAAMSQVVAQPIN"
     gene            complement(1739..2629)
                     /locus_tag="PLB86_05030"
     CDS             complement(1739..2629)
                     /locus_tag="PLB86_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HRC64531.1"
                     /translation="MSYAAPATQASQDPDLLAGLVSQVGAGGVFVLTGAGLSTDSGIP
                     DYRGRDGVRRVMPMQFGEFVGSSAARQRYWARSYVGWQRFRAASPNDGHRSLARLGDL
                     GLFEALVTQNVDGLHQSAGSADVVELHGSLAHVVCLTCTDRSARHEVQERLAVANPGF
                     VDHARHAAADGSQVSSQIRPDGDIVLADEVVESFHPPTCLVCGADTLKPDVVFFGESV
                     PRERVQRCFDALDASRSVLVLGSSLAVMSGYRFVRRAAARGIPVAIVTHGVTRGDAQA
                     TLRLDEPLAPTLGRLVAALS"
     gene            2782..3708
                     /locus_tag="PLB86_05035"
     CDS             2782..3708
                     /locus_tag="PLB86_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRC64532.1"
                     /translation="MIDAAGLRVMKAIADEGSFTAAATSLGFTQPAISQMVRRLEQRT
                     GTALVERLGRNVRLTEAGEVLARHAVTVLAALDAAEEEVAAIAGLRSGRVRLMAFPSS
                     SASLVPRALALLKKRYPEVSVRFSEGEPPESLAALRAGDCDLAVAFAYEGTDVGRGED
                     LDPFIIRPLMDDEVRLAMPPDHPLAAQSVINLADVSDDPWIAGCPRCRGHLVTLAHGA
                     GFSPNVAFETEDYVAVLGLVRAGLGVALVPDLILEAAHEDGIVARPLSPPSRRQIHAV
                     TTEDLQRVPAVAATLEALEESARSRAKVPVRS"
     gene            3785..4309
                     /locus_tag="PLB86_05040"
     CDS             3785..4309
                     /locus_tag="PLB86_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HRC64533.1"
                     /translation="MDLRPGFPTVVDEVTVRLIASVVLVIGIVALVSGAWWLYAVLAA
                     DFILRSVFGPRLSPIAQVVLRTIRPRVPAAGRPTPGAPKRFAASIGAVLTSAATILAI
                     LHATSGSAAAGTAVFAIGAIMVVFPALESAAGLCVGCVIFAQLIRLGLVPERICLECA
                     DITRRTAVASGQPA"
     gene            complement(4310..>6253)
                     /locus_tag="PLB86_05045"
     CDS             complement(4310..>6253)
                     /locus_tag="PLB86_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRC64534.1"
                     /translation="TSQVDAPVLVVGGAEWVGLQWQEGFRARGRLAPAEAGDDVVAIL
                     RATGPPELTAEAPLVARGAAYVRGRFREATARLPPDAAGLVPALVIGDTSATPVDLSE
                     AMTVTGMSHLSAVSGSNVAIVLAAAIGAARLAGVRRRWRPVLAVVVLAGFVVLARPEP
                     SVVRAAVMGVVGLVGLSASRRRAGVPALATSIVVLLCWDPWLARAYGFALSVLATLGL
                     LILARPWGERLGRLLPGRLARAGPTLAVPLAAQVMCAPVVVLLQASVSLVAVPANLVA
                     APFVAPATILGVATAVVSVIWVDAAAWLAWLAAIPTLAIAGVARVAASMPFGSLPWPA
                     GPPGALLLGGLSVLGLVTAPWLRFRLLQRPLAGLAIIAVTTAAAVPTRNVGWPMAGWS
                     LVACDVGQGDGIVLSTSAGHAVLVDAGLDPAVIDGCLRRLDVRVLDAVILTHFHADHV
                     NGLPGALRGREVREILTSAVQDPVFQVGAVGDWARAAGVAVKPLFAGDRLEWPGITAR
                     VWWPARVLHEGSIPNNGSVVMTADVKGVHVVLLGDIEREAARAVGAAIRRDPQVPGWE
                     VDVVKVAHHGSANIDPAVLDALPGAVAVISVGAENDYGHPAPSTLTDLRSRGFQILRT
                     DLDGDIAVGRPSEGTLMVATRGP"
     assembly_gap    6254..6353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6354..7397)
                     /locus_tag="PLB86_05050"
     CDS             complement(6354..7397)
                     /locus_tag="PLB86_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HRC64535.1"
                     /translation="MARTEELEQALSARAVALLERAGSTPAARNGWVPVPPPGWSRYA
                     APPGVPDAAPPDVAVRGDRSRGGGPPRRVGGRHRLPPPRVITVPAGLGGSRVRARAAA
                     VGALLAVVVVVGLIFALRVAAAARAAEPVPVAPSRTGVVARSVPMAFASGSIGRSGAM
                     GDEGATSADGAMGDASVGVEGSAGSGPIAGAEPGTIGRLLVVHVVGEVGAPGVVRVPE
                     GSRVVDVIRAAGGALASADLERVNLARAVVDGEQVHVPAPGEQILAGANGAGAGAGAP
                     AASGGSAPALINLNSADLAQLDTLPGVGPVLAQRILDWRAQHGRFTSVDELGEVSGIG
                     DTLLAQLTPKVSV"
     gene            complement(7506..8186)
                     /gene="plsY"
                     /locus_tag="PLB86_05055"
     CDS             complement(7506..8186)
                     /gene="plsY"
                     /locus_tag="PLB86_05055"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRC64536.1"
                     /translation="MSGSQVVAGLPQLVAGPLDLGSGELWRTVAVVVVAGAASFLWGG
                     INPATLIGRTLGRDVTRAGSGNPGATNAGRVLGRKWGILVGVLDVAKGFVPAFLGAAF
                     GSVWLAYVLGVAAVLGHVFSPYLRGRGGKGVATALGAILGVHPWYAVALLAVFVLVFA
                     VSRWVALGSLLAAALMLVGGLLAAFGRLPGAIGLPTMIWAWVLGLVIVLRHRDNVISR
                     LRSLGRLE"
     gene            complement(8186..9058)
                     /locus_tag="PLB86_05060"
     CDS             complement(8186..9058)
                     /locus_tag="PLB86_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRC64537.1"
                     /translation="MGVAIVTDSTAYLTPAQVAEAGAPVRVVPLHVVIGGTERREGVD
                     VRVEDLAEALRRFVPVTTSRPAPAEFLQVYEELAAAGADSIVSVHISADMSATVQGAQ
                     LAAAQSPVPVTVVDSRVLGAAMGYAVLSAAAAAGAGADAETVAAVARSRAHDATVVFY
                     VHTLEHLRRGGRIGSASALLGSALAIKPILALADGHIAPIEKVRTSSKALARMVELAG
                     DAADRARTAGRIVDIEVQHLDFAARAAEVADALAQRVPDAGEIRIGELGAVVGAHVGP
                     GTLAITVAPRPESH"
     gene            complement(9076..12033)
                     /gene="leuS"
                     /locus_tag="PLB86_05065"
     CDS             complement(9076..12033)
                     /gene="leuS"
                     /locus_tag="PLB86_05065"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRC64538.1"
                     /translation="MSDETPYRYTAALADTIEQAWQDRWENDGVFHADNPAGPWARPD
                     AVAARGPKLLVLDMFPYPSGAGLHVGHPLGYIATDVFSRFHRMQGRNVLHALGYDAFG
                     LPAEQYAVQTGQHPRKTTEDNIVIMRRQLRRLGLGFDDRRSYATIDEDYYRWTQWIFT
                     QIRESWYDPEAVRPDGSHGAARPIAELVAALESGERSVPESEGRAWTELSAREQAGVL
                     EHYRLAYPSEAPVNWCPGLGTVLSNEEVTNEGRSERGNFPVFRRNLRQWMMRITAYAD
                     RLADDLDRVEWPEKVKIMQRNWIGRSEGAMVTFPVTTAAGEAAGIEVFTTRPDTLFGA
                     TFMVLAPEHPLVDEIIPSAGWPEGTHEVWKGADAAAADSPAAAVAAYRRSTSRKSDVE
                     RQMDDRTKTGVFTGAFAANPLNGNPIPVFVADYVLMGYGTGAIMAVPAGDQRDYDYAT
                     EFELPIVDVIAPVGVSDWPGTVALGHSFTEAGVAINSSNDDVSLDGLGTVEAKARITA
                     YLQDKGIGRGTVNYRLRDWLFSRQRYWGEPFPVVWDEDGVAHSLPDSMLPLTLPDVPD
                     YSPKTYDAEDSGSEPEPPLSRVSEWVNVELDLGDGRGVRRFRRETNTMPNWAGSCWYY
                     LRYLDPTNSEALVDPANERYWIGPQANPVPGAPAGTRDPGGVDLYVGGVEHAVLHLLY
                     ARFWHKVLFDLGHLSSEEPFRTYFSQGYIQAPAFTDERGQYVQAEEVVETPGVHGAEA
                     TFTWQGQPVKREFGKIGKSLKNMVSPDEMYAVYGADTFRIYEMSMGPLELSKPWETRA
                     VVGSARFLQRLWRNVVDEITGECRVVELTETDLDPALRTLLHKTIAGVREDYAGLRFN
                     TAIAKLIELNNAVTKLPAPPRSLVEPLVQMVAPVAPHIADELWERMGRSGSLALGPFP
                     VADLALLVEDTVTCVIQIKGKVRDRVDVPADISESDLRELVLAREKVLAATADGIRTV
                     IVRPPSLVNVVPM"
     gene            complement(12225..12863)
                     /locus_tag="PLB86_05070"
     CDS             complement(12225..12863)
                     /locus_tag="PLB86_05070"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HRC64539.1"
                     /translation="MNIYAWVLLCAFAVAALADWAAVARRRPDLESVAKPAVLVILIL
                     LAWLLRADSVDYGNQLLIGLVLCLIGDVLLLGRSDRHFLAGLIAFLLAHVAYIAAFRR
                     IPGEGPIWWGVIAVAVLGVVVILTTVLPIVRASGRDGIPLVVYALVVGGMAALAWATG
                     LPVVGIGATLFLASDALIAYDRFARPLAWGHVVVHITYHLGQLLIVLGMLRT"
     gene            12938..13327
                     /locus_tag="PLB86_05075"
     CDS             12938..13327
                     /locus_tag="PLB86_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRC64540.1"
                     /translation="MAASTFEHRVTTADTAAVLGSGDLPVLATPRLINWFERATFATA
                     QTVVEAPQTTVGTVVRIEHLKASTVGAVITVSCSTPISDGRRLIFHVRALDEDGAEIA
                     VGEMHRRVVDSDRFMARFTEATPRPAD"
     gene            13356..14876
                     /locus_tag="PLB86_05080"
     CDS             13356..14876
                     /locus_tag="PLB86_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRC64541.1"
                     /translation="MSATRRIGARELLSTVLDEGSWVCWDTPPPPVAEPGSGYAADLA
                     AAAEAAGTDESVLTGEGRIRGRRVACVAGEFRFLAGSIGKAAAERITLAFERARHEQL
                     PLFAAPTSGGTRMQEGTPAFLGMVKISAAVAQFKAAGLPYIVYLRHPTTGGVLASWGS
                     LGHVTVAEPGAMIGFLGARVYEALYGRPFPDGVQVAENLHARGIIDAVVPVERLAEMA
                     ARTLSVLDARHTDQAPVLRPEASVPDPYAGLEPPASAWDSILLSRRTDRPGVKTLLKL
                     GASDVLLLRGTGQGEHDPGMLLALARFGGTPCIVLGQDRHRKTLEEPLGPGGLRVARR
                     GIRLAADLNLPLVSIIDTEGAALSKEAEEGGLAGEIARSLADLVMLPAPTLCVLLGQG
                     TGGGALAILPADRVICAQHAWLSPLPPEGASAIVHRTIERADEMAASQRVRSVDLRED
                     GIVDRIVPEHPDAASEPEAFCRRMAEAIHQEVDELWRADSGARVAARLERYRRMGT"
     gene            15075..15566
                     /locus_tag="PLB86_05085"
     CDS             15075..15566
                     /locus_tag="PLB86_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64542.1"
                     /translation="MPVIKALEARSHELVDEALGLLDHRLNPPYKQVDRDVLRRRLHQ
                     TMSTVTTALTERTPLPVIDYAREVARRCFNAGFLLEEVQTHFNALEETVWKFMVKEVD
                     AAHLPENLAMVASVIGTIKDELGRSYVELAAHHRAPAINTHKLFDGTQSVPRHPMSAK
                     AAH"
     gene            15710..16642
                     /locus_tag="PLB86_05090"
     CDS             15710..16642
                     /locus_tag="PLB86_05090"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRC64543.1"
                     /translation="MSGQSVSRALTRLADLTAEAVGSAHGGVAAAVRGVLRLGRDRSV
                     VVVGRDGTSATMRLHESRFSGMDTIGSQLLSGSYEPTTRRVLEALVTSGATVVDVGAN
                     IGYFTVLSALLAGPTGRVIAFEPEPRNHAVLTDNVARNGLRQVMVVQAACADRVGQHE
                     LSINSSETGWHRLVAAGEVGRGKRVVVEVTTVDTIVGEAPVDVVKIDVEGHEGAVVTG
                     MAQTLADNPGITVILEHSPAQARLAGLDPLAPVTLLHQAGLRHTYVVREDKAILEKID
                     VESLRSRDVKTGRSVNLVMRAQPWPADSAVPVVS"
     gene            16651..>17769
                     /locus_tag="PLB86_05095"
     CDS             16651..>17769
                     /locus_tag="PLB86_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HRC64544.1"
                     /translation="MPGFDVSQFRAQFPSLTSGLAHFDGPGGTQTPYAVGAAVAATLT
                     GPLSNRGTTGVSETNAEAAVAGFRAAYADFLGVPERGVVYGRSATALTFDVGRAVAAS
                     WRPGDEIVVSRLDHDANVRPWLIFAERAGVTVRWLDFDPATSEIIWESVESAITERTR
                     LVAVTGASNLIGTIPPVAAIAARAHAVGALVWVDGVHFAAHHLVDVGAIGADFFVCSP
                     YKFLGTHCAALGAAPELLETLRPDKLLPSSNDVPERFELGTLPYEVMAGATAAVDVLA
                     GLGGQAPESAGRRERLAASWAALHAHESRLRSIIEAGLAEFGDRVVVHSRAAQRTPTL
                     LLTFPGRRSWDAYQFLSARGIQAPAGSFYAYEPFTRLAL"
BASE COUNT         2780 a         6531 c         5881 g         2477 t
ORIGIN      
        1 gatgtttggc acatgcgtcc gaacggcata ccctgtgtgc atggacgcaa gtccatcagt
       61 gaccaccgac agggggtcct gaccaccggc ccacgagccc tcgtgagcac gcggcaggcg
      121 cccggatcgt ccgttcacat caaccggagg taggagccat ggctgaggtc accgagctgc
      181 ccgagcgcga gggcgacggg ctcaccgtgc ggcagcggcg ggttctcgag gtcatccgca
      241 actccgtcga ccggcgcgga tacccgccga gcctgcgcga gatcggcgaa gccgtcggcc
      301 tcaccagccc gtcgagcgtg gcccaccaac tcgccgccct ggaacgcaag ggctacctcc
      361 gcaaagaccc caaccgcccc cgcgcgcttg aggtcgtctc ccccgacgcc aaggccagcg
      421 accgcggtta ccgcggcagc agcggtatgc cgcccagcag cgccgacgcg tgggagcacg
      481 gcgaggctca cgacaccgag accgactccg gcgacgcccg tcccgaggcg tcctacgtcc
      541 ccgtggtggg ccgcatcgcc gccggtgggc cgatccttgc cgaggaggcc atcgaagatg
      601 tcttccccct cccccgacag atcgtcggcg aggggcagtt gttcctgctc aaggtcgtcg
      661 gcgactcgat ggtcgacgcg gcgatctgcg acggcgactg ggtcgtcgtg cggcaacagc
      721 cgacggcgga caacggcgac atcgtcgcgg cgatgatcga cggcgaggcc accgtgaaga
      781 ccttccagcg caaggccggc cgtgtcctgc tcatcccgca caacgcggca tacgagccga
      841 tcgacggtcg cgatgcgagc atcctcggca aggtgaccgc ggtcctgcgc cgtatttgag
      901 tgtcggcgcc cggagcgccc ctgacatgct gaggcgatga ccacccggct gccgttcgcc
      961 agttacctcg atcacctccg gatcgaatcc gaggccttcg ccgtcgccct gacggacgtc
     1021 cccgatgacc gtcaggtgcc gacctgcccc gactggacgg cgctggacct cgcccatcac
     1081 ctcaccgagg tccagtcctt ctgggccgcc gtcgtgagcg gtccgctgtt gaccgacgag
     1141 gccgctgagg acgtggccaa acctgcccgc gccacctcgg tgccggccca actcgaggac
     1201 ctgcgagcgg cgagcgctcg tctcgcggcc gccctggcca ccacccctgc ggacacgccc
     1261 gcctggacct ggtccacgga gcagaccgtc gggttcacct tccgacgcca ggcccacgag
     1321 gccctcgtcc accgcctcga cgcccaactc accgccggcc tgcgcactcc cgtggatccg
     1381 acgctcgcga ccgatggggt cgacgaggcg ctgcgggtga tgttcggcgg ctgcccccca
     1441 tggggtcaga tcacgcccgt tgccggccct gaggtccggc tggccacgag cgacacaggg
     1501 gtctcgtggc tgatctcgcc ggcccgcttc accggcaccg acccggacgg caccgcctat
     1561 gacgaccccg acatcgttgt ggcagagtcg gattcagggg gcgacgtcgc ggcaacgatc
     1621 accgggaccg ccgaagacct gctgtgctgg ctctggaagc ggcccaccgt cgggacgctc
     1681 tcccacacgg gcgacgagtc ggccctcgct gcgatgagcc aggtggtcgc ccaacccatc
     1741 aactgagcgc cgcgaccaac cggccgagcg tgggagcaag cggttcgtcc agccgcaggg
     1801 tcgcctgcgc atctcctcga gtcactccgt gggtgacgat ggccacgggt atgccgcgtg
     1861 ccgccgcccg ccgcacgaat cggtagcccg acatcaccgc caaggacgag ccgaggacga
     1921 ggaccgaccg gctggcgtcc agcgcgtcga agcagcgctg gacccgctcg cgcggcaccg
     1981 actccccgaa gaacaccacg tcgggcttga gggtgtcggc gccgcacacc agacaggtcg
     2041 gcgggtggaa cgactcgacg acctcgtcgg ccaggacgat gtctccgtcg ggccggatct
     2101 ggctgcttac ctgagagccg tccgcagcag cgtgccgcgc atgatcgacg aacccggggt
     2161 tggccacagc cagccgctcc tggacctcgt gccgagcact gcgatcggtg cacgtgaggc
     2221 agacgacgtg tgcaagcgat ccatgcaact cgacgacgtc ggccgagccg gcactctggt
     2281 gcagcccgtc gacgttctgg gtgaccagag cctcaaacag accgaggtcg cccagccgcg
     2341 cgagtgaccg atgcccatcg ttgggcgaag ccgcccggaa gcgttgccag ccgacatagg
     2401 acctcgccca atacctctgg cgggcagcgc tggacccaac gaactcaccg aactgcatcg
     2461 gcatcacccg acggaccccg tccctccctc ggtagtcggg gatcccggag tcggtcgaca
     2521 accctgcccc agtgaggacg aacacgcccc cggcgccgac ctgcgacacc aagccggcca
     2581 gcaggtccgg gtcctggctc gcctgcgtgg cgggcgcggc atacgacatg cccgcagtat
     2641 gccgccctcc cggactcgcg cgccgaccac cgagaccgac cggaaccggc gctcccgact
     2701 cccaccgggg atgcgacggc tgtcacatta cctaccgggt gattctgtct ctttgcaata
     2761 actcgcggtt atgattgcct catgatcgat gccgccggcc ttcgggtgat gaaggccatt
     2821 gccgacgagg gcagcttcac cgctgcggcc acgtcgctgg ggttcaccca gcccgccatc
     2881 tcgcagatgg tccgccgtct ggaacagcgc acgggcaccg cgctcgtgga gcggctcgga
     2941 cgcaacgtgc gcctcaccga agccggcgaa gtcctggccc ggcacgccgt caccgtcctg
     3001 gcggccctcg atgccgccga ggaggaggtc gcggcgatcg ccggcctgcg ctccgggagg
     3061 gtgcggctca tggccttccc ctcgtcgtca gcgtcgcttg tgccgcgcgc cttggcgctg
     3121 ctcaagaagc gctaccccga ggtgtccgtg cgtttctccg agggcgagcc gcccgaatcg
     3181 ctcgccgcgc tgcgcgctgg tgactgcgat ctggccgtcg cgttcgccta cgagggcacc
     3241 gacgtcggcc gcggcgaaga cctcgatccc ttcatcatcc ggccactcat ggacgacgag
     3301 gtccgcctcg ccatgccgcc ggaccacccg ttggccgctc aatcggtcat caacctcgcc
     3361 gatgtctccg acgacccgtg gatcgccggc tgcccgcgct gccgagggca cctggtcacg
     3421 ctggcccacg gcgcagggtt ctcccccaac gtcgccttcg agaccgagga ctacgtcgcc
     3481 gtcctcgggt tggtgcgcgc cggcctcggg gtcgcgctcg tccccgacct catcctcgaa
     3541 gccgcccacg aggacggcat cgtcgcccgg ccgctcagcc cgccgtcccg ccgccagatc
     3601 cacgcggtga cgaccgagga tctgcagcgg gtcccggccg tcgccgccac gctcgaggca
     3661 ctggaagaga gcgcccgctc ccgcgccaag gtccccgtgc gttcctaggg tgcagcggcg
     3721 gcccggctgt ccacatcgcg ggcacgtcac ataaggaacc cttattcgtt ccataatcaa
     3781 tgacatggat cttcgccctg ggttccccac tgtcgtcgac gaggtcaccg ttcgcctgat
     3841 cgcgagcgtt gtgctcgtga tcggcatcgt cgccctcgtc tcgggggcct ggtggctgta
     3901 cgccgtcctc gccgccgact tcatcctgcg cagtgtcttc gggccgaggt tgagcccgat
     3961 cgcccaggtc gtgctgcgca cgattcggcc tcgcgtgccg gctgcgggcc gcccgacgcc
     4021 tggagccccc aagcggttcg cggcgagcat cggtgctgtc ctgacgtcgg ccgcgacgat
     4081 cctggccatc ctccacgcga cgagcggatc ggccgctgcg gggaccgccg tcttcgccat
     4141 cggcgcgatc atggtcgtct ttcccgcgtt ggagtcagcg gctgggctgt gcgtcgggtg
     4201 tgtcatcttc gctcagctga tccggctcgg cctggtgccg gagcgcatct gcctggagtg
     4261 cgccgacatc acccgccgga cggctgtggc ctccggtcag cctgcctgat cacgggccgc
     4321 gagtggccac catgagtgtg ccttcgctcg gccgaccgac cgcgatgtcc ccgtcgaggt
     4381 cggtccgcag gatctgaaag cctcgactgc gcaggtcggt gagcgtggac ggcgcgggat
     4441 ggccgtagtc gttctccgcc cccacactga tcaccgcgac ggcaccggga agcgcgtcaa
     4501 gcacggctgg gtcgatgttg gccgacccat gatgggccac cttgacgacg tcgacctccc
     4561 aacctggcac ctgcggatct cgacggatcg ccgcaccgac agcgcgggca gcctcccgct
     4621 cgatgtcgcc cagcagcacc acgtggacac ccttgacgtc ggcggtcatg acgaccgacc
     4681 cgttgttggg gatcgatccc tcgtgcagga ctcgggcggg ccaccacacc cgcgccgtga
     4741 tccctggcca ctccagccga tctccggcga agagcggctt gaccgcaacc ccggcggccc
     4801 gggcccagtc gccgacggct cccacctgga agaccgggtc ttgcaccgca gaggtgagga
     4861 tctcgcgcac ctcccggccc cgaagcgccc caggcaggcc gttgacgtgg tcggcatgga
     4921 agtgggtgag gatcacggcg tcgaggactc gcacgtccag tcggcgcaga cagccgtcga
     4981 tcactgccgg gtccagcccg gcgtcgacga gcacggcatg cccggcagac gtggacaaga
     5041 cgatgccgtc gccctgcccg acgtcgcagg ccaccaggga ccagccagcc atcggccacc
     5101 cgacgttccg cgtcgggacg gccgcggcgg tggtcaccgc gatgatcgcc agtcccgcaa
     5161 gagggcgctg cagcaggcgg aagcgcagcc acggagcagt aacgagcccg agcaccgaca
     5221 agccaccgag cagtagggca ccaggcggcc cggccggcca cggaaggctg ccgaacggca
     5281 tactcgcggc cactcgggcc acacccgcga tggcgagagt cgggatcgca gccagccacg
     5341 cgagccacgc ggcggcatcc acccagatca cggaaacgac ggctgtcgcc acgccgagga
     5401 tcgtggccgg cgcgacgaac ggtgcggcga ccaggttggc cggaaccgcc accagagaga
     5461 ccgaggcctg caggaggacg acgaccgggg cacacatgac ttgggcagcc agtggcacgg
     5521 ccagggtcgg tcccgcgcgg gccaaccgcc caggcagcag ccgccccaat cgttcgcccc
     5581 agggacgagc gaggatcagc agcccgagcg tggccagcac cgacaacgcg aatccgtagg
     5641 cccgggcgag ccacgggtcc caacacagca ggacgacgat cgaggtagcg agcgccggca
     5701 cgccagcgcg gcgacgggag gcactcaaac cgacgagccc aaccaccccc ataacggccg
     5761 ctcgcacgac gctcggctcc ggacgcgcca acaccacgaa gccggccagg acgacgactg
     5821 cgaggaccgg tcgccatcga cgccggactc ccgccagccg cgcggctccg atggccgcgg
     5881 ccaggacgat agccacgttc gagccgctaa ccgcggacag gtgcgacatg ccggtgacgg
     5941 tcatcgcttc ggacaggtcg acgggtgtcg ccgacgtgtc cccgatgacc aacgcgggga
     6001 cgaggcccgc cgcatccgga gggagccgcg ccgtggcctc gcggaaccga ccccggacgt
     6061 atgccgcccc cctcgccacc agcggcgcct cggcggtgag ctccggcggc cccgtggccc
     6121 gaaggatggc gacgacatcg tccccggctt cggccggagc cagccgccct cgggcacgga
     6181 agccctcctg ccactgcagc ccgacccact cggcaccacc aacgaccagg acgggggcgt
     6241 cgacctggct ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcacacg
     6361 ctcaccttgg gcgtgagctg cgccagcaac gtgtctccga taccgctcac ctcccccaac
     6421 tcgtccaccg aggtgaaccg gccgtgttga gcgcgccagt cgaggatccg ctgggcgagc
     6481 accggcccca caccgggcaa cgtgtccaac tgggcaagat cggcggagtt gaggttgatc
     6541 agcgcgggag cgctgccacc ggaggccgcc ggagcgccag cacccgcgcc cgcgccgttg
     6601 gctccggcga ggatctgctc acccggcgcg gggacgtgga cctgctcccc atcgacgacc
     6661 gcgcgcgcga ggttgacccg ttcgaggtcg gcagaggcca gcgcccctcc ggccgctcgg
     6721 atcacatcga caacccggga accctccggc acccgcacga cccctggcgc gccgacctca
     6781 ccgacaacat ggacgacgag gagccgcccg atggtgcccg gctctgcgcc ggctatgggg
     6841 cccgaccccg ccgaaccctc gacacccact gaggcgtcgc ccatcgcccc gtccgcactc
     6901 gtcgctccct cgtcgcccat cgcccccgag cgcccgatcg accccgacgc gaacgccatc
     6961 ggcaccgacc gcgccaccac ccccgtccgg ctcggcgcca ccgggaccgg ctcggccgcc
     7021 cgcgcagccg ccgccacccg aagcgcgaag atcagcccga caaccaccac cacagccagc
     7081 agcgccccca cggccgctgc gcgcgcccgc accctcgacc ctcccaagcc ggcgggcacg
     7141 gtgatcactc tgggcggagg cagccgatgc cgtccgccca ctcgccgcgg tgggccgccc
     7201 ccgcgtgacc ggtcaccgcg cacggccacg tcgggcggcg cggcatcagg aacgcccggg
     7261 ggcgcggcat acctcgacca gccaggcggt gggaccggga cccaaccgtt acgggccgcc
     7321 ggcgtcgagc cagcgcgctc cagcaacgcc accgcccgcg ccgacagggc ctgctccagt
     7381 tcctcagtgc gcgccatgcc cggacggtat gccgcccgct caccttcccg tcaggccccc
     7441 accttcgcct gtggacagtg ggacgagcgt cagcaccctt gtggacaacc cggcccacca
     7501 gggaactact ccaggcgccc caacgaccgc agccgggaga tgacgttgtc ccgatggcgc
     7561 agcacgatga ccagccccaa cacccaggcc cagatcatcg tcggcagccc gatggcgccc
     7621 ggcagtcggc cgaaggcggc gagcagcccc ccgacgagca tgagcgctgc cgcgagcagc
     7681 gaccccaagg ccacccagcg cgagaccgcg aagacgagca cgaagacggc aaggagggcg
     7741 acggcatacc aggggtgcac gccgaggatg gcacccaagg cggtcgcgac ccccttgcca
     7801 ccgcgcccgc gcaggtaggg cgagaacacg tgccccagca ccgccgcaac cccaagcaca
     7861 taggcgagcc acacggagcc gaacgccgcc ccgaggaacg ccgggacgaa acccttggcc
     7921 acgtcgagga cccctacgag gatcccccat ttgcggccca gcacgcggcc ggcgttggtc
     7981 gcaccggggt tgcccgagcc cgcgcgggtg acgtcgcgac ccagcgtccg cccgatgaga
     8041 gtggctgggt tgatgccacc ccagaggaac gacgcggccc ctgcgacgac caccacggcg
     8101 acggtccgcc acagctcacc ggaccccagg tcgaggggcc cggccaccaa ctgcggcaga
     8161 ccggcgacca cctgcgaccc gctcactagt ggctctccgg ccgaggcgcc accgtgatcg
     8221 ccagcgttcc cggacccaca tgcgcgccga cgacggcgcc caactcgccg atccggatct
     8281 cgcctgcgtc gggcacccgc tgcgccagcg cgtcggcgac ctcggcggcc cgtgccgcga
     8341 agtccaggtg ctggacctcg atgtccacaa tccggcccgc cgtgcgggct cgatccgctg
     8401 cgtcgccggc caattcgacc atccgcgcca acgccttgct cgacgtgcgc accttctcga
     8461 tcggggcgat gtgtccgtcg gcgagggcga ggatcggctt gatcgccagc gccgagccga
     8521 gcagggccga cgccgagccg atccgcccac cgcgccgcag atgttcgagc gtgtggacgt
     8581 agaagaccac cgtcgcgtca tgtgctcgcg aacgcgccac cgccgcaacg gtttccgcat
     8641 cggctccggc acccgccgcg gcggcggccg acaacacggc ataccccatg gcggccccga
     8701 gcacccgcga gtcgacgacc gtcaccggca ccggcgactg cgcggcagca agctgcgcac
     8761 cctggaccgt agccgacatg tcagccgaga tatgcacgga gacaatggaa tccgcacccg
     8821 cagcggccaa ctcctcatag acctggagga actcagccgg cgctggccgc gacgtcgtca
     8881 cggggacgaa tcgccgcagc gcctccgcca gatcctcgac ccgcacgtcg acaccttcac
     8941 gacgctcggt gccgccgatg acgacgtgca gggggacgac ccgcaccggc gcgccagcct
     9001 cggccacctg agccggcgtg aggtatgccg tggagtcggt gacgatcgcg acgcccatcc
     9061 gggtgcctcc tgacctcaca tggggacgac gttgaccagc gacggcggcc ggacgatgac
     9121 ggtgcgaatg ccgtccgccg tggcagccaa caccttctca cgggcgagca ccaactcgcg
     9181 caggtccgac tcggagatat cggccggcac gtccacccga tcgcggacct tgcccttgat
     9241 ctggatgacg caggtgaccg tgtcctcaac caggagagcg aggtcggcga ccgggaacgg
     9301 cccgagcgcc agcgacccgc tccgacccat ccgctcccac aactcgtcgg cgatgtgcgg
     9361 cgcgacgggc gccaccatct gcaccaacgg ctcgacgagc gaccgcggcg gcgcaggcaa
     9421 cttcgtcacc gcgttgttga gctcgatgag cttggcgatg gccgtgttga accgcagccc
     9481 cgcatagtcc tcacgaacac cggcaatcgt cttgtgcagc aacgtccgca gggcggggtc
     9541 gagatcggtc tcggtcagct cgaccacccg gcactcaccc gtgatctcgt cgacgacgtt
     9601 gcgccacaac cgctgcagga agcgcgccga gccgacgacg gctcgggtct cccacggctt
     9661 ggacaactcc agcggaccca tcgacatctc gtagatgcgg aacgtgtcgg cgccgtacac
     9721 ggcatacatc tcgtccgggc tgaccatgtt cttcagcgac ttgccgatct tgccgaactc
     9781 gcgcttgacc ggctgtccct gccacgtgaa cgtcgcctcc gcgccgtgca cgccgggggt
     9841 ctccacgacc tcctcggcct ggacatactg cccacgctcg tcggtgaatg ccggggcctg
     9901 gatgtagccc tgcgagaagt aggtccggaa cggctcttcg ctggacaggt ggcccagatc
     9961 gaagagcacc ttgtgccaga accgtgcata gagcaggtgc agcaccgcgt gctcgactcc
    10021 accgacatag aggtcgacgc cgcccgggtc gcgcgtcccc gcaggagcac cagggaccgg
    10081 attggcttgc ggtccaatcc aataccgctc attggccggg tcgaccagtg cctcggagtt
    10141 ggtcgggtcg aggtagcgca ggtagtacca gcaggatccg gcccagttgg gcatcgtgtt
    10201 ggtctcgcgg cggaaccgcc gcaccccgcg cccatcgccc aggtccaact cgacgttgac
    10261 ccactcgctc acgcgggaca gcggcggctc gggctcgctg cccgagtctt ccgcgtcgta
    10321 ggtcttcggc gagtagtccg ggacatcggg cagcgtcaac ggcaacatcg agtcgggcaa
    10381 cgagtgcgcg accccgtcct cgtcccacac cacggggaac ggctcgcccc aatagcgctg
    10441 ccggctgaac agccagtcgc gcaggcggta gttgaccgtc cctcggccaa ttcccttgtc
    10501 ctgcaggtat gccgtgatcc tcgccttcgc ctcgaccgtc cccagcccgt cgagcgacac
    10561 gtcatcgttg gacgagttga tggcgacccc ggcctcggtg aacgagtgcc ccagggcgac
    10621 ggtgccgggc cagtccgaca cccccacggg ggcgatgaca tcgacgatcg gcaactcgaa
    10681 ctcagtggcg tagtcgtagt cgcgctgatc gccggccggc accgccatga tcgcgccggt
    10741 cccgtaaccc atgagcacgt agtcggcgac gaacaccgga atcgggttgc cgttcaacgg
    10801 gtttgcggcg aaggccccag tgaacacccc agtcttggtg cggtcgtcca tctgccgctc
    10861 gacatcgctc ttgcgcgagg tggagcgtcg gtatgccgcg accgccgccg ccggggagtc
    10921 cgccgccgcc gcgtcggcgc ccttccacac ctcatgggtg ccttcgggcc agcccgcgct
    10981 cgggatgatt tcgtcgacca gcggatgctc gggagcgagc accatgaagg tcgctccgaa
    11041 cagcgtgtcg ggccgggtcg tgaagacctc gatgccggcg gcctcgcccg cagccgtcgt
    11101 caccgggaag gtcaccatgg cgccctcgga ccggccgatc cagttgcgct gcatgatctt
    11161 gaccttctcg ggccactcga cccggtccag gtcgtcggcc aaccgatcgg catacgccgt
    11221 gatccgcatc atccactgac gcaggttgcg ccggaacacc gggaagttgc cccgctcgga
    11281 gcgtccttcg ttggtgacct cctcgttgga caacacggtg cccagaccgg gacaccagtt
    11341 gactggcgcc tccgagggat aggccaaccg gtagtgctcc agcacgccag cctgctcgcg
    11401 tgccgacaac tcggtccatg cccgcccctc cgactcgggc acagaccgct cccccgactc
    11461 caaggccgcc accaactcgg cgatggggcg agccgcccca tgcgagccgt cggggcgcac
    11521 tgcctcgggg tcgtaccaac tttcccggat ctgggtgaag atccactggg tccatcggta
    11581 gtagtcctcg tcgatcgtcg cgtaggagcg tcggtcgtcg aatcccagtc ccagacgccg
    11641 caattggcgg cgcatgatga cgatgttgtc ctcggtcgtc ttgcgaggat gctgaccggt
    11701 ctgcacggca tactgctccg cgggcagccc gaaggcgtcg taacccaggg catgcaggac
    11761 gttgcgaccc tgcatccggt ggaagcgcga gaagacgtcg gtcgcgatgt agcccaaggg
    11821 gtgcccgacg tgcaggcccg ctcccgaggg gtaggggaac atgtccagga cgagcagttt
    11881 gggaccccgc gcggcgacag cgtccggacg cgcccatggc ccggcggggt tgtccgcgtg
    11941 gaacaccccg tcgttctccc aacggtcctg ccacgcctgc tcgatcgtgt cggccagcgc
    12001 ggcggtgtag cgatacggcg tttcgtcgct catcgtgctc tcttgtcctt cggcaactgt
    12061 cgtggtcgtg ggccggtcga acgccggtgc ctggtgggca aaaagaaacc cctcacgcag
    12121 gaggggctgc cgcgctgacg tccgactact cagcgcggcc cggtaaggag cagactccgt
    12181 gccacggccc cagggtagcg cacccccatg cgcacctacc tgcctcaggt gcgcagcatc
    12241 cccaggacga tgagcaactg cccaaggtgg taggtgatgt ggacgaccac gtgcccccag
    12301 gccagcggcc gggcgaagcg gtcataggcg atgagcgcgt cgctcgcgag gaacagcgtg
    12361 gccccgatcc cgacgacggg cagcccggtc gcccaggcga gggcggccat ccctcccacc
    12421 accagcgcat agaccaccag gggtatgccg tcccggccac tcgcccggac gatcggcagc
    12481 accgtggtga ggatcaccac gacccccagc acggcgacgg cgatcactcc ccaccagatc
    12541 ggcccctctc cggggatccg gcggaaggcc gcgatatagg ccacatgggc caggaggaag
    12601 gcgatcaacc cggcgaggaa gtgccgatcc gatcgcccga gcagcagcac atcgccgatg
    12661 agacacagca cgaggccgat gagcaactgg ttgccgtagt cgaccgagtc cgctcgcagc
    12721 agccaggcca gcaggatgag gatgacaagg accgcgggct tggccacgga ctcgaggtcg
    12781 ggtcggcgcc gggcgaccgc agcccagtcc gcgagcgctg cgacagcgaa cgcgcacagc
    12841 agcacccagg catagatgtt cattggcaca caccctctcg catcagggcg cattgccggg
    12901 ccagcggcgc gctcggggcc gccctagact cgatgacatg gcagcgtcca cgttcgagca
    12961 ccgggtgacc acggccgaca cggccgccgt cctcggcagc ggcgatctcc ccgtgctggc
    13021 caccccgcgc ctgatcaact ggttcgagcg ggccaccttc gccaccgcgc agacagtcgt
    13081 ggaggcacca cagaccaccg tcggcacggt ggtgcgcatc gagcacctca aggccagcac
    13141 ggtcggggcg gtgatcaccg tgtcgtgctc caccccgatc agtgacggcc gccgactgat
    13201 cttccacgtg cgggccctcg acgaggacgg tgccgagatc gcggtgggcg agatgcaccg
    13261 ccgcgtcgtc gactccgatc gattcatggc gcggttcacc gaggcgacgc cgcgccccgc
    13321 cgactagcca caccctcacg acgaaaggcc cggccatgag cgcaacgcgg cgcatcgggg
    13381 caagggagtt gctgagcact gttctcgacg agggcagttg ggtctgttgg gacactccgc
    13441 cgcccccggt ggctgagccc ggcagcgggt atgccgccga cctcgccgcc gcggccgagg
    13501 ccgccggcac cgacgagtcg gtcctcaccg gcgaagggcg catccggggc cgacgtgtgg
    13561 cctgtgtcgc cggtgagttc cgcttcctcg ccgggtcgat cggcaaggcc gccgccgagc
    13621 ggatcacgct ggccttcgag cgcgctcgac acgagcaact gcccctcttc gccgctccga
    13681 cctctggggg cacgcggatg caggaaggca cgccagcctt cctcggcatg gtcaagatct
    13741 cggccgcggt cgcccagttc aaggccgccg ggctgcccta catcgtctat ctgcgccacc
    13801 cgacgaccgg tggggtgctg gcgtcgtggg ggtcgctggg ccacgtcacg gtcgccgagc
    13861 cgggcgcgat gatcggcttc ctcggcgccc gcgtctacga ggcgctctac ggacggccgt
    13921 tccccgacgg cgtccaggtc gccgagaacc tccacgcccg cggcatcatc gacgcggtgg
    13981 tgccggtcga gcggctcgcc gagatggcgg cgcggacgct ctcggtgctg gacgcgcggc
    14041 ataccgacca ggcgccggtc ctccgccccg aggcgagcgt tcccgacccg tatgccggtc
    14101 tcgaaccgcc cgcgagcgcg tgggactcca tcctgttgtc ccgtcgcacc gaccggcccg
    14161 gggtgaagac cctgctcaaa ctcggcgcgt ccgacgtgtt gttgttgcgt gggaccggac
    14221 agggcgagca cgaccccgga atgttgttgg cgctggcccg cttcggtggg acgccgtgca
    14281 tcgtcttggg tcaggatcga caccgcaaga cgctcgagga gccgctcgga ccgggtgggt
    14341 tgcgggtggc gcgtcgtggc atccggctgg ccgccgacct caacctgccc ctggtgtcga
    14401 tcatcgacac cgagggcgcg gcgctgtcca aggaggccga ggagggcggg ctggccggcg
    14461 agatcgcccg gtccctcgcc gacctggtca tgctcccggc gcccacgctg tgcgtcctgc
    14521 tcgggcaggg caccggcggc ggcgccttgg cgatcctgcc cgccgaccga gtgatctgcg
    14581 cccagcacgc ctggctgtcc ccgttgcccc ccgagggagc ctcggcgatc gtgcatcgca
    14641 cgatcgagcg ggccgacgag atggcggcct cgcagcgggt ccgctcggtg gatctgcgcg
    14701 aggacgggat cgtcgaccga atcgttccgg agcaccccga tgccgcgtcg gagcctgagg
    14761 cgttctgccg acggatggcc gaggctatcc accaggaggt cgacgagttg tggcgggctg
    14821 attccggggc tcgggtggcc gcgcgactgg agcgctaccg ccggatgggc acctagttca
    14881 cccgctgttc acgcacccgc gggacctctg gcccgacgct tctgacgccg gtccgtcgta
    14941 cccttggttc aacgggcaca gccgccaggg gcagcacgac gctgtgcgct tccccgctcg
    15001 gctggcccaa cgtgtgcggt atgccgccga ggcagcctca cagcgacgcc ccacccgcga
    15061 aaggcagaac tcacgtgccg gtcatcaagg cccttgaggc ccgttcccac gagctcgtcg
    15121 acgaggccct gggcctgctc gaccaccggc tcaacccgcc ctacaagcag gtcgaccgtg
    15181 acgtgctgcg ccgccggctg catcagacga tgtcgacggt gacgaccgcg ctcaccgagc
    15241 gcacgcccct gccggtcatc gactacgccc gcgaggtcgc gcgccgttgt ttcaacgccg
    15301 ggttcctgct ggaagaggtg cagacgcact tcaacgcgtt ggaagagacc gtgtggaagt
    15361 tcatggtcaa agaggttgac gccgcccacc tgccggagaa cctcgccatg gtcgcctccg
    15421 tcatcgggac gatcaaggac gagctgggcc ggtcctacgt cgagttggcc gcccaccacc
    15481 gggccccggc catcaacacc cacaagctct tcgacggcac ccagagcgtc ccgcgtcacc
    15541 cgatgtccgc caaggctgct cactgacgtt cggcggcccc ttggcccgca ctggtcggca
    15601 cgcaccgact gacgggctga cgtgatcccg gtgtttccgg ctggctgggc cgagcgctag
    15661 cgttgtcctt cgtggggaac gtggccagcc gcgaaacgaa ggagcttcat tgtccggtca
    15721 gtcggtgtct cgcgcgctga cgcgcctggc cgacctcacc gctgaagccg tcggctcggc
    15781 ccatggtggc gtcgccgccg ccgtccgagg ggtccttcgc ctgggtcgtg atcgcagcgt
    15841 tgtcgtggtg gggcgcgacg ggacgtccgc caccatgcgg ctgcacgaat cccgcttctc
    15901 cgggatggac acgatcggca gccaattgct ttctggcagt tacgaaccca cgacccgtcg
    15961 agtgttggaa gccctcgtca cctccggggc caccgtggtc gatgtcggcg ccaatatcgg
    16021 ctacttcacc gtcttgtcgg ccctgctcgc cggacccacc ggtcgcgtga tcgcgttcga
    16081 gcccgagccg cgcaaccacg cggtcctcac cgacaacgtc gcccgcaacg ggctgcgcca
    16141 ggtgatggtg gtgcaggcgg cgtgcgccga ccgggtcggg cagcacgagt tgtcgatcaa
    16201 ctcctccgag accgggtggc accgactggt cgccgccggt gaggtcgggc gcggcaaacg
    16261 ggtcgtcgtg gaggtgacca ccgtcgacac catcgtcggc gaagcgccgg tcgacgtcgt
    16321 caagatcgac gtcgagggcc acgagggcgc ggtcgtcacc gggatggccc agaccttggc
    16381 cgacaatccg gggatcaccg tcatcctcga gcactccccc gcccaggccc gcctcgccgg
    16441 gctcgacccg ctcgcaccgg tgacgctgtt gcaccaggcg gggctgcggc atacctatgt
    16501 ggtccgcgaa gacaaggcga tcctcgagaa gatcgacgtc gagtccctgc gctcgcgcga
    16561 cgtcaagacc gggcgctcgg tcaatctcgt catgcgcgcc cagccctggc ccgcggactc
    16621 cgcggtgccg gtcgtctcct aggaggtccg atgcccggct tcgacgtgag tcagttccgc
    16681 gcgcagttcc cctccctgac ctcggggctc gctcacttcg acgggcccgg cggaacgcag
    16741 acgccgtatg ccgtgggagc cgccgtcgcg gccaccctca ccgggccgct gtccaaccgc
    16801 ggcaccacgg gggtgtctga gaccaatgcc gaggcggcgg tcgcagggtt ccgggcggca
    16861 tacgcggact tcctgggggt gcccgagcga ggggtcgtct acgggcgaag cgcgacggca
    16921 ctgaccttcg acgtggggcg cgcggtggcg gcgagctggc gtccgggtga cgagatcgtc
    16981 gtctcacgcc tggaccacga cgccaacgtc cggccgtggc tgatctttgc cgagcgcgcc
    17041 ggcgtgacgg tgcgctggct ggactttgat ccggcgacgt ccgaaatcat ctgggagagt
    17101 gtcgaatccg cgatcacgga gcggacccga ctggtggcgg tgaccggggc gtccaacctc
    17161 atcgggacga tcccgccggt ggcggcgatc gcggctcggg cccatgcggt gggcgcgctc
    17221 gtgtgggtgg acggggtgca ttttgcggcg caccacctcg tcgacgtcgg ggcgatcggg
    17281 gccgacttct tcgtgtgctc gccctacaag ttcctcggga cgcactgcgc agccctgggc
    17341 gctgcccctg agctgttgga gacgctgcgg ccggacaagc tcctgccgtc gagcaacgac
    17401 gtgcccgagc ggttcgagct ggggacgctg ccctatgagg tgatggccgg ggcgaccgct
    17461 gccgtcgacg tgctggctgg cctgggcggc caggctcccg agagcgccgg gcggcgggaa
    17521 cgattggccg cgtcgtgggc ggcgctgcac gcccacgaga gccggctgcg gtcgatcatc
    17581 gaggccggtc tggccgagtt cggcgaccgg gttgtcgtcc actcccgggc tgcccagcgg
    17641 accccgaccc tcttgctcac cttccccggc cgacgctcgt gggacgccta ccagttcctg
    17701 tccgcgcgcg gcatacaggc tcctgcgggg tccttctatg cctacgagcc gttcacgcgg
    17761 ttggcgttg
//
LOCUS       DAORPW010000039        17744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_592_length_17744_cov_685.162700, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000039 DAORPW010000000
VERSION     DAORPW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 17744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17744
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_592_length_17744_cov_685.162700"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(306..1124)
                     /locus_tag="PLB86_05100"
     CDS             complement(306..1124)
                     /locus_tag="PLB86_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC64545.1"
                     /translation="MTEPDGGSDLLGAMRTRAVRDGDSYVINGAKLWITNGNVADVAL
                     VYAKTDPSAAHGGVSAFVVPTDTPGFEARRVPCRGLGSLMPTTSISFTDVRVPASGLL
                     GAEGEGFTVAMNAMDYGRLTVSARAVGLAQACLDAALSYADTRVAFGEKISSFQMVKK
                     QLADMTCEVAAARALVQAAAAAYQGGMPPARPSSIAKYYAADVANRAAQATAVIFGGM
                     AFSDELPIGLYLNYAKLWQTGEGSAKHPGDPDRLDALGWKDMDRHVVRRRPLEG"
     gene            complement(1155..1418)
                     /locus_tag="PLB86_05105"
                     /pseudo
     CDS             complement(1155..1418)
                     /locus_tag="PLB86_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            1600..2367
                     /locus_tag="PLB86_05110"
     CDS             1600..2367
                     /locus_tag="PLB86_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRC64546.1"
                     /translation="MASTTPSRPAAPARPPKAAMMVAQRIVRDSLRDGLKPGDLMSPE
                     RTMLEKYQTGRGTLREALRLLEFQGVIALKPGPRGGPVLQNPDASHLASTVVLLMQIN
                     EAPFRTIVEVRSAMEPMISSLAAVRMNEDDLADLAATIDQMRTDMDDQFSFLEANKRF
                     HDVIAWSSGNVLFGYIVDSLLGIMDGTVIGIHYPTHRRQAILVAHQEIYEALKSRDPQ
                     ASQARMQEHIDAYVKYAERKYPQLLQETTPWDQRYFS"
     gene            complement(2462..3190)
                     /locus_tag="PLB86_05115"
     CDS             complement(2462..3190)
                     /locus_tag="PLB86_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC64547.1"
                     /translation="MLEITDLYAGYGQIEAVRGLSLSVDPGRVTLLLGANGAGKSTTL
                     RTVAGLHPPTSGTVVLNGVDVTGQPAHKMVRHGVSLVPEGRRVLAPMTVLENLRLGAY
                     TADKAAYARTLAEVNAMFPILADRSNGAAGLLSGGEQQMLAFGRALMSQPKIMLLDEP
                     SMGLAPAVTDRILANVRTMANSGIGILMVEQNAEAGLEVADHVIAVARGEVVFAGPAE
                     AARANASVLRAFLGEAALAGESGA"
     gene            complement(3183..3971)
                     /locus_tag="PLB86_05120"
     CDS             complement(3183..3971)
                     /locus_tag="PLB86_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC64548.1"
                     /translation="MSAAEATATRPVLEIRDLAVHFGGVKAVDRLSMRLDEGRIYGII
                     GPNGSGKSTLLGAVTRLTPLTSGEILFDGEPFHTVHASQIAAKRIARTFQTVRLLPDV
                     SVRDNIQLGADAARGDVDERRGWWARNFGTHTSPAVASAIARTRLGGFEDYRPAEMSY
                     GTQRRVEIARALALDPRLLLLDEPTAGMNQRERGEISTLLKELRDEGLTQLLVEHDVQ
                     MMVDTCDYVFAMNLGTVIAQGRPEDVVRDPQVQEAYLGKKFRHA"
     gene            complement(3968..4900)
                     /locus_tag="PLB86_05125"
     CDS             complement(3968..4900)
                     /locus_tag="PLB86_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC64549.1"
                     /translation="MSDFYAANLVLIQSTFIGILLALSVQVPMRAGVFSFAGAGSYGI
                     GAYVSAILVLKTGMPSLLAILISGIVAMVVGLLLGLLVAHLGGLYLAMATVSFDLIVS
                     VLAINGGDLTGGVTGLFGVLSDVTIFHLIGLALLGIVGVALTERGRVGRRVDAVRDDP
                     ELAQSVGISVRRYRLAAFVVSGLLGSVAGSLNSLVRSTISPSDIGFSLIVLALTMIIV
                     GGAQSWKGAVLGAVIFTWLPPLLAGFGQLQHVVYGVIVATAAIFVPGGLYGIYSEARY
                     RRTRAKRKPPQGEAPAHEQALAISVPNGDAGRSA"
     gene            complement(4897..5787)
                     /locus_tag="PLB86_05130"
     CDS             complement(4897..5787)
                     /locus_tag="PLB86_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC64550.1"
                     /translation="MLQDLLGALGLASIYLLFALGMSLTWGTIDILNFSHGSIFMFSA
                     FTAHLLIPYVPMGFVGVALVCIVVGALMSLLVQVVAFEQIIKRARNRNTAERQILIGG
                     IGVAIIPLAIAQEVTKSNPFGFIDSGFEVTTWTFMGLRGTNILLITLTAAAALAVGTA
                     MWLRRSRHGLALRSIGVDSEVSAMMGVDRRRLAIVTMAVSGGLAGLAACLFTYTLGAI
                     TPESGDTLLIKAFACIILGGVGSTGGVVLGSVLLAVTETVVLTRTSGLWVEAVAFGLI
                     FLVLLVKPSGLFGRKEVRRT"
     gene            complement(5857..7053)
                     /locus_tag="PLB86_05135"
     CDS             complement(5857..7053)
                     /locus_tag="PLB86_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC64551.1"
                     /translation="MDWTAVRLGRRGAVAVVAAAALGMSACSSSSGTSSTSAAAGGGL
                     PATINVVSINPQTGPAAFAGLAANKGYDLAIKEINDSKFLGSTTLTFSKVDTKGDIKT
                     AASSASTAIADKNVSAIFGSVSSQEAIAMSPLVQQAGLPTIYTQAGSDGVVVGDATYR
                     ATPLMRTYYSLVKKYVQEKGWKSVGIIHTAAAPTLVDIGTKALPAIATELGMTVTTTV
                     AVQGTTQDFAAPISQVLASKPDGVAILLVGAQNPTAMKQLRQAGYTGDVLGNSGASAG
                     NLKPAGADGAGMVWPVDFNFQQKAEASQKFVKAYQAANSGENPLNYAAEAYDAAWFLA
                     KSIKAAGSADRAKIKAAMATVASEKFDGALGNGLTWKDRDIVVPGVMIRWDGTGDVLL
                     YEGSAS"
     gene            complement(7213..8160)
                     /locus_tag="PLB86_05140"
     CDS             complement(7213..8160)
                     /locus_tag="PLB86_05140"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC64552.1"
                     /translation="MLNAIPRLDLRDLQRARTERIHLARAGQAGWQPPEGVEISERQV
                     PAGSRPRVRTFTPTRAPSHPDPTDHPPRPALVWLHGGGHVMGTVWQDDPRLCRWALDT
                     GAVVVAVDWRWAPEHPYPAALDDACAALRWCLAGADDLDIDPARVVVAGASSGGGLAA
                     GAALRCRDEGDPPLRAQVLIYPMLDDRGDTPSARTCTDPRVWNDESNSRAWAAYLSGW
                     SRPIPGYAAPARATDLTGLPPTWLATGALDLFRDEDLEYAARLLAAGVPTELHVYAGA
                     VHGFDLFAPNAGVSVRFRAELDAAMRAALTADTGWASLR"
     gene            complement(8253..8714)
                     /locus_tag="PLB86_05145"
     CDS             complement(8253..8714)
                     /locus_tag="PLB86_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRC64553.1"
                     /translation="MAGLYFEDFEIGKEYVHPWSRTVTETDTILYCGLTMNPAPIHLD
                     AHYMETTIHGQRLVPSLYTGGLVGGMIVNELTLGTTLGNLGYTKFDFPKPVFHGDTIR
                     AETTILDKRESKSRTDSGIVFFEHRGINQRGEIVHIAHRAGLMLKRPVADG"
     gene            complement(8786..9688)
                     /locus_tag="PLB86_05150"
     CDS             complement(8786..9688)
                     /locus_tag="PLB86_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRC64554.1"
                     /translation="MQPYRSVLFVPAHKTSWFAKAIDAGPDAVCFDLEDSVPEHLKAG
                     AREQVAEAVAAYSQSHPALGLFVRPNSLGTRLTGLDLEAAVVPGLTGVFAPKVDSALD
                     VVRYDALLDHFEARAGVTGLEYIIPVETIHGIQNCEEIAAASERVGAMIGPTAEHADI
                     AKAVGFEWTPEGLESMVHRTKILLATRAVDRHPLTALWERIHDLDGLRDFSIRSRRMG
                     FRGQIVLHPSHVPVVNAVYTPDAEQIAFYRGLLETYEAAAARGDGAVMYGEVHVDKAH
                     ADKATEWLARVDTLASLNGGLPSA"
     gene            complement(9724..10893)
                     /locus_tag="PLB86_05155"
     CDS             complement(9724..10893)
                     /locus_tag="PLB86_05155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC64555.1"
                     /translation="MADYTPNPLHEDIRKAVREMMEPFDDQYWMEHDVDHAFPWEFYN
                     AVAKAGWLGITIPEEYGGGGLGVTEASIVEYEISASGGGMNACSAVHIGIFGFDPIIR
                     HGSEQLKQRFLPRVPTGDLHISFAVTEPDAGTDTTGITTFAKKVDGGFRVSGKKVWIT
                     KAQEAERMLVLVRTTKREDCVKRTDGMTLLFAEMDPNHVQVREIPKMGRNAVNTNEVF
                     IDDLFVADEDVVGEVGKGFRTLLTGLNAERIVAANAALGIGRAAVRRATAYAKEREVF
                     GRPIGQNQAIAHPLAEAQMRLDAAELMVYKAAWLLDNGYECGPEANMGKFLAADAAFF
                     AADRALQTHGGFGYGKEFHVERYFREARLKRIAPISQEMVLNYLSEHVLGLPRSY"
     gene            complement(10919..11719)
                     /locus_tag="PLB86_05160"
     CDS             complement(10919..11719)
                     /locus_tag="PLB86_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC64556.1"
                     /translation="MTQSTGSIRVSDRGDSRWVVIDNPAARNAYDQEMATRLAEVIEA
                     STDVRSVVITGSPGAFCAGGMLAGLSAPTQHGMRELYRSSLRLFDAVRQCPRPVIAAV
                     NGAAAGGGNELVIACDLAIAAESATFGQTGPRVGSAPVTGATNVLGVQIGEKRAKEMA
                     MLCRRYTAAQALDLGLINAVVPDADLESTVQEWCEHIATLSPRYLEITKVSSNIWWNS
                     ARDSFAQGIGMLVQAIGSPDMVEGATAFLEKRRPDFRLDQGELPPDPR"
     gene            11737..>12464
                     /locus_tag="PLB86_05165"
     CDS             11737..>12464
                     /locus_tag="PLB86_05165"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRC64557.1"
                     /translation="MQQFGQGLRAPDFVVEASDALGEGPCWDPRDDSLGWVDIRGRAV
                     HAWWPATGRRERRALPDEVSLALPRVSGGRVVTQVDTIAVADGIHLREVCRVPADRVH
                     TRLNDGCVDRDGALWVGTYSLRGEAEAVLLHVDPSSGEVTVVVDGLVASNGLAWSADG
                     ERLFAVDTGRGRLDVYDRVDGARPALRPGRLLVDFAGGVGRPDGIAIDADGGVWVAMW
                     GGGEVRRYAPGGDLDVVIRVPVTYP"
     assembly_gap    12465..12564
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12565..13193)
                     /locus_tag="PLB86_05170"
     CDS             complement(<12565..13193)
                     /locus_tag="PLB86_05170"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HRC64558.1"
                     /translation="MVVLSKPKNPSHPPQSLTITDADIRPTTELLWRIHRTSGLHPAA
                     WNDFRTYGPLPTMRWDPHPQPTGIHLGRGVLYASIDLRTAVAEVFQSTRRIDPFTGDP
                     HVTTFIPRRPLRLLRLYSEDATWLLNHDASHSLMHGPKSTCRNWACGILDAHRLDGLW
                     VQSTMTGHATTVLFEPSAPKLPLRARDSAPLTAPAIMAALDDIATSLGWR"
     gene            complement(13181..13750)
                     /locus_tag="PLB86_05175"
     CDS             complement(13181..13750)
                     /locus_tag="PLB86_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64559.1"
                     /translation="MLSIAEVFDRYDLELSESDFARELARSLADAPAPTDVSARLLTD
                     LQESGGLTDPARLSTWSQRDDRDLRVAVQSTMLSDLLAATYSASQTAELLSISRSRLS
                     HKTKSVPLLTALVGRHRRYYRWQFSEGHIVPGLGILADHLTGDEHPLDIATMMTSRSD
                     DLDGRTPVDFLVTGGNPVLVARLLEGLSF"
     assembly_gap    13771..13870
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13871..14136)
                     /locus_tag="PLB86_05180"
     CDS             complement(<13871..14136)
                     /locus_tag="PLB86_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64560.1"
                     /translation="MICCQFIFKPGTYDEDFHRLDGQIDEFARALPGFERAETWFSED
                     RSVVNAVYYFADLQSVRELARFPQHQEAKSQVTRWYDGYQIIVS"
     gene            complement(14133..14897)
                     /locus_tag="PLB86_05185"
     CDS             complement(14133..14897)
                     /locus_tag="PLB86_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRC64561.1"
                     /translation="MTSPLHATGGGAHLHSALTGTYLDGAHLDESVANLRPDYRALLI
                     VATGVRGGPSDAASDEALTRAETLAKARLNGAPPESLPEIAAWRDAFLGFGVKPRQAR
                     SSVEALLRRVDAGLPRIDRLTDLYNAISIEHLIPLGGENLDRYVGPPRLVRADGDETF
                     ETVADGIPTTVTVDPGEVVWRDDLGVTCRRWNWRQCVRTRLDPSTTNVLFILDGLDPV
                     GTDRLEAAGAALVDALTRHAPDATATTRLIGAGSPR"
     gene            complement(14894..15511)
                     /locus_tag="PLB86_05190"
     CDS             complement(14894..15511)
                     /locus_tag="PLB86_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRC64562.1"
                     /translation="MKDPRAAEPATASAIGMAIGRRVRGARTDLGWTLDQLAQRAGVS
                     RRLLVNVEQGSTNPTIGTLLRLSDALGVGLPALVDTSGDPSAPVTVRRAADQTPVWTS
                     PSGGSATMVAGTAPPDVCELWDWRLGPGDAHTSEAHRAGTRELLLVLSGTVDLQVGDT
                     EQRLKTGDSATFDGTTPHAYRNASATRPARFTLAVFEPNLSKEHG"
     gene            15661..>16173
                     /locus_tag="PLB86_05195"
     CDS             15661..>16173
                     /locus_tag="PLB86_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64563.1"
                     /translation="MPLRLIGYWRNEDHPDYPEPSDLIDRDWPDQDRDDIASYLRGGI
                     FFRGFMGLSPCRICGQHNGSSEFTDGVLVWPEGLAHYLTEHAVRLPPSIENYVRQQMD
                     YLDSQKPDIAWWTSGAPPTTSIDHPPLAPLRRLAWFGNVQLVQDPDRRHPQLLIAGDT
                     LHLHADGPKAR"
     assembly_gap    16174..16273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16274..17478)
                     /locus_tag="PLB86_05200"
     CDS             complement(<16274..17478)
                     /locus_tag="PLB86_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HRC64564.1"
                     /translation="MTDHLNVYLDGILAGRLAQASGGALSFSYDDLYLTRRVPTPLSL
                     SMPLSGSRHSNRVVAAWLEGLLPDNLGVREEWGRRFEVSPRNPFALLRHVGRDAAGAV
                     QVLPADVDPPDAAQRTGKVRRLSHREVNDLLVGLTVRDAGWEVGAGAGRWSLAGVQNK
                     VALHRLDDGDWGVPEDSTPTTHILKPTRAETRFADLHVNEFVCLRAAGHLGLRTAHVE
                     LVDFGGAKTLVSTRYDRGRGPDGAWLRLHQEDLLQAMNYPPSKKYQSDGGPSVKSAAA
                     LFATLALPDRDLVRFSFFDALAFNVLVGGTDAHAKNYSLLLRGSRVALAPLYDVASYA
                     PYLSKGEAARSSMKMGRTWQVRDLTAEDWADVGISLGLDPDDALSRVERLRVGLPEAV
                     AAAADEAPEP"
     gene            complement(17475..>17744)
                     /locus_tag="PLB86_05205"
     CDS             complement(17475..>17744)
                     /locus_tag="PLB86_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC64565.1"
                     /translation="MLTTVRDLGAAVRHRRREARLTQAMLAQRAGVSRQWLSGFESGK
                     NPAVELGKVLDVLTALDLALELVSAPPPETGTDVDPFAGFFAERT"
BASE COUNT         2677 a         5645 c         6367 g         2755 t
ORIGIN      
        1 gcacgaatct cagcccggcg agatgccgag tcacgagtcg cttcgctgct gtccataacc
       61 ccgacgctaa cagcgaccac cgacagaccc cccaccccaa aaggcctctc ccacaaccga
      121 acacatcaca aacacgtaac gatccccccg gcgcccacgc cggcgctggc gccagcgccc
      181 gtgccacgac ggccaccgcg ggcgggcagc cgctgaggcg tggtcggcca ggccaattcg
      241 tcacaggccc tagcctccgg gggcacacaa agccggcctg ccgacagggt tccgcccagg
      301 cccattcagc cctccaacgg ccggcggcgg acgacgtggc ggtccatgtc cttccacccg
      361 agtgcatcca ggcgatcagg atcgcctgga tgtttcgctg acccctcgcc ggtttgccac
      421 agcttggcgt agttgagata cagcccgatc ggcagttcgt cgctgaacgc catcccgccg
      481 aagatcaccg ccgtcgcctg ggcggcccgg ttcgcgacgt ccgcggcgta gtacttcgcg
      541 atcgagctcg ggcgggcggg cggcataccc ccttggtatg ccgcggccgc ggcctggacg
      601 agcgcgcggg cggccgcgac ctcgcaggtc atgtcggcca gctgcttctt gaccatctgg
      661 aagctgctga tcttctcccc gaaggcgacc cgcgtgtcgg cgtaggagag cgcggcgtcg
      721 aggcacgcct gggcgaggcc gaccgcccgg gccgagacgg tgaggcggcc gtagtccatc
      781 gcgttcatgg cgacggtgaa gccctcgccc tccgccccga gcagccccga ggcgggcacg
      841 cggacgtcgg tgaaactgat ggaggtcgtc ggcatgagcg accccaaccc gcggcagggc
      901 acccgtcggg cctcgaaccc gggcgtgtcg gtcggcacga cgaaggcgct gacgccaccg
      961 tgagcggcgc tcgggtcggt cttggcatag acgagggcga cgtcggcgac gttgccgttg
     1021 gtgatccaga gcttggcgcc gttgatgacg taggagtcgc cgtcgcggac cgcgcgcgtg
     1081 cgcatcgcgc cgagcaggtc ggagccgccg tcgggctcgg tcatcgcgtt ggcgccgagg
     1141 gcctcgccgg cgatcaaggc cggcacccag cgcgccacct gctcggcggt gccccagttg
     1201 gcgatggtca acggcacggt cgccgcctgc aggttcatcg ccgcggtcag tgggggatag
     1261 acccaggcga gttgctcggc gacggccgcc agggcgacga acccggcacc cgttccgccg
     1321 acgctttcgg ggaagcaaca accgaagagc cccagctcgc ccatctcgcg atacagcgcc
     1381 cgggggaact cgccagcgag ctccaactca cgaatctgcg gacggacgcg cgcgaggcct
     1441 tagtttctga ctgcggcagc catctcatca gcggtatcgt caccagttgg tgccactcgc
     1501 gacaattcag acattgcggt gcacctcctc agcagccatc tggcgcgccc tcggcccttt
     1561 gctgttaatg tttaccattg ttctgcagag aggcatgaga tggccagcac cactccctcg
     1621 cggccggcgg caccggcccg gcccccgaag gccgccatga tggtcgccca gcgcatcgtc
     1681 cgcgacagcc tgcgtgacgg actcaagccc ggcgacctca tgtcgcccga gcgcaccatg
     1741 cttgagaagt accagaccgg ccgtggcacg ctgcgagaag cgttgcggtt gttggagttt
     1801 cagggagtca tcgccctcaa gccgggcccc cgtggtgggc cggtcctgca gaaccccgac
     1861 gcctctcacc tggccagcac cgtcgttctg ctcatgcaga tcaacgaggc gccgttccgc
     1921 acgatcgtcg aggtccgctc cgcgatggag ccgatgatca gctcgctcgc cgccgtgcgg
     1981 atgaacgagg acgacctggc cgacctcgcc gcgacgatcg accagatgcg caccgacatg
     2041 gacgaccagt tctcgttcct cgaggccaac aagcggttcc acgacgtcat cgcctggtcg
     2101 tcgggcaacg tgctcttcgg ttacatcgtc gactcgctgc tcggaatcat ggacggcacg
     2161 gtcatcggca tccactaccc gacgcaccgg cgccaggcga tcctcgtggc ccaccaggag
     2221 atctacgagg ctctcaagtc ccgcgacccg caggcgtccc aggctcggat gcaggagcac
     2281 atcgacgcct acgtgaagta tgccgagcgg aagtacccac agttgctgca ggagacgact
     2341 ccctgggacc agcgctactt cagctgaccg gctggggtgg gcgctgcccg ccccggcgcc
     2401 gctgactctg cggcttgctg atccactgcc gcggcgcgtg gccgggcagg tcaggtcagg
     2461 gtcacgcgcc cgactcgccg gccaacgccg cctcgccgag gaaggcccgc agcaccgagg
     2521 cgttcgcccg agccgcttcg gcagggccgg caaagacgac ctcaccacga gcaacggcga
     2581 tgacgtggtc ggcgacctcc aggcccgcct ccgcgttctg ctccaccatg aggatcccga
     2641 tgcccgagtt ggccatcgtg cgcacgttcg cgaggatccg gtcggtgacc gccggcgcca
     2701 gccccatgga cggctcgtcc agaagcatga tcttgggctg ggacatcaac gcacgaccga
     2761 aggcgagcat ctgctgctcc ccgccggaga gcagcccggc ggcgccgttg ctgcggtcgg
     2821 cgaggatggg gaacatcgcg ttcacctcgg caagcgtgcg cgcgtatgcc gccttgtcgg
     2881 ctgtgtaggc gccgagccga aggttctcca agaccgtcat gggcgcgagg acccgacggc
     2941 cttcgggcac cagcgagacc ccgtgtcgca ccatcttgtg cgcaggctgg ccggtgacgt
     3001 cgaccccgtt gagcacgacg gtgcccgagg tgggcgggtg caggccggcg acggtccgca
     3061 gggtggtcga cttgccggcg ccgttggccc ccagcagcag ggtgacccgg cccgggtcga
     3121 cgctcaggct caaaccacgc acggcctcga tctggccgta accggcatac aggtcggtga
     3181 tctcaagcat ggcggaactt cttcccgagg taggcctctt ggacctgcgg gtcgcggacg
     3241 acgtcctccg gccggccctg ggcgatgacg gtgccgaggt tcatcgcgaa gacgtagtcg
     3301 cacgtgtcga ccatcatctg cacatcgtgc tcgacgagca gttgggtgag gccctcgtcg
     3361 cgcagttcct tcagcagggt ggagatctcg ccgcgctccc gctggttcat gccggcggtg
     3421 ggctcgtcga gcagcaagag gcgggggtcc agggccaggg cgcgggcgat ctccacccgg
     3481 cgctgggtgc cgtaagacat ctccgctggg cggtagtcct cgaagccgcc gagccgggtg
     3541 cgggcgatgg ccgaggcgac cgcgggggag gtgtgggtgc caaagttgcg agcccaccag
     3601 cctcggcgtt cgtcgacatc gccgcgggcc gcgtcggcgc cgagctggat gttgtcgcgg
     3661 acgctgacgt cggggagcag ccgcaccgtc tggaaggtcc gggcgatccg cttggccgcg
     3721 atctgcgagg cgtggaccgt gtggaacggc tcgccgtcga agaggatctc gcccgaggtc
     3781 agcggagtca gccgggtgac ggcgcccagg agggtgctct tgcccgagcc gttcggtccg
     3841 atgatcccgt agatgcgacc ctcgtccagg cgcatcgaca accggtcgac ggccttgact
     3901 ccgccgaagt ggaccgcgag gtcgcggatc tccaggaccg gccgggtggc cgtcgcctct
     3961 gccgcgctca tgccgacctc cctgcgtcgc cgttggggac cgagatggcc agggcttgtt
     4021 cgtgagccgg tgcctcgccc tgcggcggct ttcgcttggc gcgggtgcgc cggtaacggg
     4081 cctcgctgta gatgccgtag agcccgccag gcacgaagat cgccgcggtc gccacgatga
     4141 cgccatagac gacgtgctgc aactggccga agcccgccag caacggcggc aaccaggtga
     4201 agatgacggc cccgaggacg gcgcccttcc acgactgggc gcctccgacg atgatcatcg
     4261 tcagggccag gacgatgagg ctgaagccga tgtccgaggg ggagatggtg ctgcggacca
     4321 gcgagttgag ggagccggcc accgagccga ggagcccgct gacgacgaag gccgccaacc
     4381 ggtagcgccg cacgctgatg ccgaccgact gcgccaactc cgggtcgtcg cggaccgcgt
     4441 cgacccgtcg gcccacccgg ccgcgctcgg tgagggcgac cccgacgatg cccagcaggg
     4501 ccaggccgat caggtggaag atcgtcacgt cggacagcac tccgaacagg ccggtgacgc
     4561 cgccggtgag gtcgccacca ttgatcgcga ggaccgagac gatgaggtcg aaggacaccg
     4621 tcgccatggc gaggtagagc ccgccgaggt gggcaaccag cagcccgagc aggaggccga
     4681 ccaccatcgc gacgatgccg gagatgagga tggcgagcag ggacggcata cccgttttga
     4741 gaacgaggat ggccgagacg taggcgccaa tgccgtagga ccctgcgccg gcaaaggaga
     4801 agactcccgc gcgcatgggg acctggacgc tgagcgcgag caggatgccg atgaaggtcg
     4861 actggatgag gacgaggttg gccgcgtaga agtcgctcat gtgcgacgca cctccttgcg
     4921 gccgaacagt ccagatggct tgacgagcag gacgaggaag atgaggccga aggccaccgc
     4981 ttcgacccag aggccgctcg tgcgggtgag gacgaccgtc tcggtgacgg ccagcaggac
     5041 cgagccgagg acgacgccgc cggtcgagcc gacgccgccg aggatgatgc aggcgaaggc
     5101 cttgatgagc agcgtgtcgc cgctctcggg ggtgatcgcg ccgagggtgt aggtgaacag
     5161 gcaggcggcc agaccggcca agcctccaga cacggccatg gtgacgattg ccagccgacg
     5221 gcggtccaca cccatcatcg ccgagacttc cgagtcgacg ccgatggagc gcagggccag
     5281 cccgtggcgc gaccggcgca gccacatcgc ggtgccgacg gccagggccg cagctgcggt
     5341 gagggtgatg agaaggatgt tggtgccgcg cagccccatg aaggtccagg tcgtcacctc
     5401 gaaccctgag tcgatgaacc cgaacgggtt gctcttggtg acctcctgcg cgatggccag
     5461 cgggatgatc gccacgccga tgccaccgat gaggatctgt cgctcggccg tgttgcggtt
     5521 gcgggcgcgt ttgatgatct gttcaaaggc gaccacttgg accaggagcg acatcagggc
     5581 gcccacgacg atgcagacga gcgcgacccc gacgaagccc atgggcacgt aggggatgag
     5641 caggtgggcc gtgaacgcgg agaacatgaa gatcgagccg tgggagaagt tgaggatgtc
     5701 gatcgtgccc cacgtgaggc tcatgccgag ggcgaagagc agatagatag acgccaaccc
     5761 cagcgcgccg agcaggtctt ggagcactgc accgtccttt cgtcccgaag ggacgggcgg
     5821 ccgcggggga tgccccgcga ccgcccgtgt ggatccttag ctggcggagc cttcgtagag
     5881 aaggacgtcg ccggtgccat cccagcggat catgacgccg gggaccacga tgtcgcgatc
     5941 cttccaggtc agcccgttgc cgagggcgcc gtcgaacttc tcgctggcca ccgtggccat
     6001 ggcggctttg atcttggcac ggtcggcgct gccggcggcc ttgatcgact tggcgaggaa
     6061 ccaggcggcg tcgtaggcct cggccgcgta gttgagcggg ttctcgccgg agttcgccgc
     6121 ctggtaggcc ttgacgaact tctgcgaggc ctcggccttc tgctggaagt tgaagtccac
     6181 cggccagacc atccccgcac cgtccgcacc ggccggcttg aggttgcccg cgctggcgcc
     6241 cgagttgccc aggacgtcac cggtgtatcc ggcctgacgc agttgcttca tcgccgtggg
     6301 gttctgggcc ccgacaagga ggatggccac gccatcgggc ttgctggcga ggacctgcga
     6361 gatcggggct gcgaagtcct gcgtggtgcc ctggacggca acggtggtcg tcaccgtcat
     6421 gccgagctcc gtggcgatgg ccggcagggc cttggtgccg atgtcgacca gggtgggggc
     6481 ggcggccgtg tggatgatgc ccacggactt ccaacccttc tcctggacgt acttcttcac
     6541 cagcgagtag tacgtgcgca tcaggggggt ggcgcggtag gtcgcgtcac cgacgacgac
     6601 gccgtcgctg ccggcctggg tgtagatggt cggcaggccg gcctgttgca cgagcggcga
     6661 catggcgatc gcctcctggc tcgacaccga gccgaagatc gcgctgacgt tcttgtcggc
     6721 gatcgccgtt gatgcggagc tggcagcggt cttgatgtcg cccttggtgt cgaccttgct
     6781 gaaggtcagc gtggtgctgc ccaagaactt gctgtcgttg atctccttga tggccaggtc
     6841 gtaacccttg ttggcggcca gtcccgcgaa ggccgccggg ccggtctgcg ggttgatcga
     6901 gacgacgttg atcgtggccg ggaggccacc gcccgccgct gctgaagtcg agctcgtccc
     6961 cgaagagctc gaacaggccg acatgcccag agccgcggca gcgactactg ctacggctcc
     7021 ccgtcggccg agacgcacag ccgtccagtc catcgcgtcc tcctttagaa gatggtaatc
     7081 atgcacatga tccgcacgat gcgctgactc tacgtcccga tgaccgacct gtaaaggagt
     7141 tgccgttaat ctgcgccgtt ccgtgatccg ggcggcatcc tgccgagtgt gggtcgcagc
     7201 ccgccgaact ggtcaccgca gactggccca gccggtgtct gcggtgaggg cggcccgcat
     7261 ggcggcatcg agctcggccc ggaaccgcac ggagacacca gcattcggcg cgaagagatc
     7321 gaagccgtgg acggcaccgg catacacatg cagctcggtg ggcaccccgg cggcgagcag
     7381 tcgcgcggca tactcaaggt cctcgtcgcg gaacagatcg agggcgccgg tggccagcca
     7441 ggtgggcggc aggccggtga ggtccgtggc gcgggcgggc gcggcatacc cggggatggg
     7501 tcgggaccac cccgagaggt atgccgccca cgcgcggctg ttgctctcgt cgttccagac
     7561 gcgggggtcg gtgcacgtgc gggccgaggg cgtgtcgccg cggtcgtcga gcatcggata
     7621 gatgaggacc tgggcgcgca ggggtgggtc gccctcgtct cggcaacgca gagcggcgcc
     7681 cgcggccagt ccgccgccgg agctcgcgcc agcgacgacg acgcgggccg ggtcgatgtc
     7741 caggtcgtca gcgccggcga ggcaccagcg caacgcggcg caggcgtcgt ccagcgctgc
     7801 gggataaggg tgttcgggtg cccagcgcca gtcgacggcg acgacgactg cgccggtgtc
     7861 gagggcccag cggcacaacc gcgggtcgtc ctgccagacc gtgcccatga cgtggccacc
     7921 gccatgcagc cagacgagcg cgggccgggg cgggtggtcg gtgggatcgg ggtgggacgg
     7981 cgcgcgcgtc ggcgtgaagg tgcggacgcg aggccgagag cctgcgggca cctgccgttc
     8041 ggagatctcg acgccctcgg gcggctgcca cccggcctgc ccggcgcgtg cgaggtggat
     8101 gcgctcggtg cgggcgcgct gcagatcacg caggtcgagc cgggggatgg cgttgagcac
     8161 cgccccgacc tcagggtcga gggggcccgg cagcaggggg tgctccgtgg gttggtccca
     8221 gtcgctcagt cgccagccac tcagccggtc gctcagccgt cggcgacggg tcgcttgagc
     8281 atgaggccgg cgcggtgggc gatgtggacg atctcgccgc gctggttgat gccgcggtgc
     8341 tcgaagaaga cgatgcccga gtcggtgcgg ctcttggact cgcgcttgtc caggatcgtc
     8401 gtctcggcgc ggatcgtgtc gccatggaag accggcttgg ggaagtcgaa cttcgtgtag
     8461 ccgaggttgc ccagcgtcgt gcccagggtg agttcgttga cgatcatgcc gccgaccagg
     8521 ccgccggtgt agaggctggg gacgagccgc tggccgtgga tcgtcgtctc catgtagtgc
     8581 gcgtcgaggt ggatcggcgc ggggttcatg gtgagcccgc aatagaggat cgtgtcggtt
     8641 tcggtgaccg tccgcgacca ggggtggacg tattccttgc cgatctcgaa gtcctcgaag
     8701 tagaggcccg ccatgatgga tctccttgct gctctggggg tttgacgcta ggggttggac
     8761 gctggggttt gacgctaggg gtgggtcagg cggacgggag gccgccgttg agggaggcga
     8821 gggtgtcgac ccgcgccagc cactcggtgg ccttgtcggc gtgcgccttg tcgacgtgga
     8881 cctcgccata catcacggcg ccatcgcccc gcgccgccgc cgcttcgtag gtctccagca
     8941 gcccgcgata gaacgcgatc tgctcggcat ccggggtgta gaccgcgttg acgaccggca
     9001 catgactggg gtgcaggacg atctgcccgc ggaagcccat tcgccggctg cggatgctga
     9061 agtcgcgcag cccgtcgagg tcgtggatgc gctcccacag cgcggtgagc ggatgccggt
     9121 cgaccgcgcg ggtcgcgagc aggatcttgg tgcggtgcac catcgactcc agcccctcgg
     9181 gcgtccactc gaagccgacc gccttggcga tgtcggcgtg ctcggcggtg gggccgatca
     9241 tcgccccgac ccgctctgag gccgccgcga tctcctcgca gttctggatc ccgtggatgg
     9301 tctccacggg gatgatgtat tccagcccgg tcaccccggc gcgcgcctcg aagtggtcca
     9361 gcagcgcgtc gtagcgcacg acgtcgagcg ccgagtcgac cttgggggcg aacacccccg
     9421 tcagcccggg gacgaccgcc gcctcgaggt ccagcccggt cagccgggtg cccagcgagt
     9481 tgggtcgcac gaacagtccc agcgcgggat gggactggga gtatgccgcg accgcctccg
     9541 ccacctgctc acgggcgccg gccttgaggt gctcggggac cgagtcctcc aggtcgaagc
     9601 acacggcgtc gggcccggcg tcgatggcct tggcgaacca cgacgtctta tgggccggaa
     9661 cgaacaggac ggaacggtag ggctgcacgg tcgtggtctc cttcgactgc cgcgagggac
     9721 agatcagtaa ctgcgcggca gcccgaggac gtgctcgctg aggtaattga gcaccatttc
     9781 ctggctgatc ggggcgatcc gcttgagccg ggcctcgcgg aagtagcgct cgacgtggaa
     9841 ctccttgcca tagccgaacc cgccatgcgt ctgcagggcc cggtcggcgg cgaagaaggc
     9901 cgcgtcggcg gcgaggaact tgcccatgtt ggcctcgggc ccgcactcgt agccgttgtc
     9961 cagcagccag gccgccttgt agaccatgag ctcggcggca tccaggcgca tctgcgcctc
    10021 ggcgaggggg tgggcgatcg cctggttctg cccgatcggc cgtccgaaga cctcgcgttc
    10081 tttcgcgtat gccgtcgcgc ggcgcacggc cgcacgcccg atgccgaggg cggcgttggc
    10141 ggcgacgatc cgctcggcgt tgagcccggt gagcagcgtc cggaagccct tgccgacctc
    10201 gccgacgacg tcctcgtcgg cgacgaaaag gtcgtcgatg aagacctcgt tggtgttgac
    10261 ggcgttgcgg cccatcttgg ggatctcgcg gacctgcacg tggttggggt ccatctcggc
    10321 gaacaacaac gtcatgccgt cggtgcgctt gacgcagtcc tcgcgcttgg tcgtgcggac
    10381 cagcacgagc atccgctcgg cctcctgcgc cttggtgatc cacaccttct tgccgctcac
    10441 ccggaagccg ccgtcgacct tcttggcgaa ggtggtgatg ccggtcgtgt cggtgccggc
    10501 gtccggctcg gtcacggcga aggagatgtg caggtctccg gtgggcacgc gggggaggaa
    10561 gcgctgcttc aactgttcgg agccgtgccg gatgatcggg tcgaagccga agatgccgat
    10621 gtgcacggcg ctgcaggcgt tcatcccgcc gccggaggcc gagatctcgt attcgacgat
    10681 cgatgcctcg gtgacgccca ggccgccgcc gccgtattcc tcagggatgg tgatgccgag
    10741 ccagcccgcc ttggcgaccg cgttgtagaa ctcccacggg aaggcgtggt cgacgtcgtg
    10801 ctccatccag tactggtcgt cgaagggctc catcatctcc cggacggcct tgcggatgtc
    10861 ctcgtgcaga gggttgggcg tgtagtcggc catgacgtcc ttcgcggtgg tgggcgggtc
    10921 agcgtgggtc tggggggagc tcgccctggt ccaggcggaa gtccgggcgg cgcttctcga
    10981 ggaaggccgt ggcgccctcg accatgtcgg ggctgccgat cgcctgcacg agcatgccga
    11041 tgccctgggc gaagctgtcg cgcgcggagt tccaccagat gttcgagctg accttggtga
    11101 tctccaggta gcgggggctg agcgtcgcga tgtgctcgca ccactcctgc accgtcgatt
    11161 cgaggtcggc gtcggggacg accgcgttga tgagcccgag gtccagcgcc tgggctgcgg
    11221 tgtagcgccg gcacagcatc gccatctcct tggcgcgctt ctcgccgatc tgcacgccga
    11281 gcacgttcgt cgccccggtg accggcgccg agccgactcg cggcccggtc tggccgaagg
    11341 tcgcgctctc ggccgcgatc gccaggtcgc aggcgatgac caactcgttg cctccaccgg
    11401 ccgcggcacc gttaacggcc gcgatgaccg ggcgcgggca ctgccgcacg gcgtcgaaga
    11461 gccgcagcga cgaccgatag agctcacgca tcccgtgctg ggtcggcgcg ctgagcccgg
    11521 cgagcatgcc gccggcacag aacgcgcccg gcgagccggt gatgacgacg gagcggacgt
    11581 cggtcgaggc ttcgatcacc tcggcgagac gggtcgccat ctcctggtcg taggcgttgc
    11641 gcgcggcagg gttgtcgatg accacccatc ggctatcgcc ccgatcggag acccggatgc
    11701 ttcccgtcga ctgcgtcacg gtctcataat aatcatatgc agcaatttgg ccaagggttg
    11761 cgggctcccg acttcgtcgt cgaggcctcc gacgccctcg gcgaaggccc ctgctgggac
    11821 cctcgcgacg actcgctcgg ctgggtcgac atccgcggcc gggcggtgca cgcgtggtgg
    11881 cccgccacgg ggcgacgcga gcgcagggcg ctgcccgacg aggtgagcct ggccctcccg
    11941 cgcgtgtcgg ggggccgcgt ggtcacccag gtggacacga tcgccgttgc ggacggcata
    12001 cacctgcggg aggtatgccg tgtgcccgcc gaccgcgtcc acactcgcct caacgacggg
    12061 tgcgttgacc gcgatggtgc gctgtgggtg gggacctatt cgttgcgtgg tgaggcggaa
    12121 gccgttctcc tgcacgtcga tccgtcgtcg ggcgaggtga cggtggtggt cgacgggttg
    12181 gtggcgagca acgggctggc ctggtcggcc gatggtgagc ggctgttcgc ggtcgacacc
    12241 gggcggggac ggctggatgt ctatgaccgg gtcgacgggg cgcggccggc gctgcggcct
    12301 ggtcggttgc tcgtcgactt cgccggtggc gtggggcggc cggacgggat cgcgatcgac
    12361 gccgacggtg gcgtgtgggt ggcgatgtgg ggtggcggcg aggtccggcg gtatgccccg
    12421 ggcggggacc tggacgtggt gatccgggtg ccggtcacct atccnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnncgccag ccgaggctgg tggcgatgtc gtcgagcgcg
    12601 gccatgatgg ctggcgcggt caacggggcg gaatcgcgcg cccgcagcgg gagtttgggc
    12661 gcggagggct cgaacagcac cgtcgttgcg tggccggtca tcgtcgattg cacccacagc
    12721 ccatccaggc gatgggcgtc gaggatgccg cacgcccaat tgcggcacgt cgacttgggg
    12781 ccgtgcatca acgagtggga cgcgtcgtgg ttcagcagcc acgtggcgtc ctcggagtag
    12841 aggcgcaaca gccgcagggg ccggcgcggg atgaacgtcg tcacatgggg gtcgccggtg
    12901 aatgggtcga tgcggcgcgt cgattggaag acctcggcca ccgcggtgcg taggtcgatc
    12961 gaggcataaa gaactccccg gccgagatgt atgccggtcg gctgggggtg ggggtcccac
    13021 cgcatcgtgg gcagcggacc gtaggtgcgg aaatcgttcc aggcggcggg atggagcccg
    13081 gaggttcggt ggatgcgcca cagcagttcg gtggtgggcc ggatgtctgc gtcggtgatc
    13141 gtgaggctct gaggcgggtg gctcgggttc ttgggcttgc tcagaacgac aatccttcga
    13201 gcaggcgcgc gaccagcacc gggttgccgc cggtgactag gaagtccact ggggtgcggc
    13261 cgtcgaggtc gtcggagcgt gacgtcatca tggtggcgat gtccagcggg tgttcgtcgc
    13321 cggtcagatg gtcggccagg atgcctagcc cggggacgat gtgtccctcg ctgaactgcc
    13381 agcggtagta gcgacggtgc ctgccgacca gcgcggtgag cagtgggacg gatttggtct
    13441 tgtgggacag ccgcgagcgg ctgatgctga gcagctccgc ggtttgggac gccgagtagg
    13501 tcgccgccag caagtcgctg agcatggtcg actgcaccgc cacccgcagg tctcggtcgt
    13561 cgcgttggct ccacgtgctc agccgggccg ggtcggtgag cccgcccgac tcctgcaggt
    13621 cggtcagcag ccgcgccgac acgtctgtgg gtgccggggc gtcagccagc gagcgggcca
    13681 actccctggc gaagtcgctc tcgctgagct ccaggtcgta ccggtcgaac acttctgcga
    13741 tgctcaacat cgtctacctc cgacccctag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn tcgctgacga tgatctggta gccgtcgtac cagcgggtga cctggctctt
    13921 ggcctcctgg tgctgcggga agcgggcgag ctcgcggacc gattggagat cggcgaaata
    13981 gtagacagcg ttgacgacac tgcggtcctc ggagaaccag gtctctgcgc gctcgaatcc
    14041 gggcagtgcg cgcgcgaatt cgtcgatctg cccgtcgagg cgatggaagt cctcgtcgta
    14101 ggtcccgggc ttgaaaatga attggcagca gatcatcgcg ggctccctgc gccgatcagg
    14161 cgagtggtgg cggtggcgtc gggggcgtgc cgggtgaggg cgtcgacgag tgcggcgccg
    14221 gcggcttcga ggcggtcggt gccgacgggg tcgaggccgt cgaggatgaa caggacgttg
    14281 gtggtggagg ggtccaggcg ggtgcggacg cactgccgcc agttccagcg gcggcaggtg
    14341 accccgaggt cgtcgcgcca caccacctct ccggggtcga ccgtcacagt ggtgggtatg
    14401 ccgtccgcga ccgtttcgaa ggtctcgtca ccgtcggcgc ggaccaggcg gggcgggccg
    14461 acgtagcggt cgaggttctc cccgccgagg gggatgaggt gctcgatgga gatggcgttg
    14521 tagaggtcgg tgagtcggtc gatgcggggg agcccggcgt cgacgcggcg aaggagggcc
    14581 tcgacgctgc tgcgagcctg gcggggtttg accccgaagc cgaggaaggc gtcgcgccag
    14641 gcggcgatct cggggagcga ctcggggggc gcgccgttga ggcgggcctt ggcgagggtc
    14701 tcggcgcggg tgagggcctc gtcgctcgcg gcgtcgctgg ggccgccgcg cacgccggtg
    14761 gcgacgatga gcagggcgcg gtagtcgggg cgcaggtttg cgacgctctc gtcgaggtgc
    14821 gcgccgtcga ggtaggtgcc ggtgagggcg gagtgcaggt gtgcgccgcc gccggtggcg
    14881 tgcagggggc tggtcatccg tgctccttgg agaggttggg ctcaaagacg gcgagcgtga
    14941 agcgcgccgg cctcgtggcg gaggcgttgc gataggcgtg gggcgtggtg ccgtcgaagg
    15001 tcgccgagtc gccggtcttg agccgttgct cggtgtcgcc gacctgcaga tcaaccgtgc
    15061 cggagaggac gaggagcagt tctcgggtgc cggcgcggtg ggcctcgctg gtgtgagcgt
    15121 cgccggggcc cagccgccag tcccatagct cgcagacgtc gggtggggcg gtgccagcga
    15181 ccatggtggc cgacccgccg ctggggctgg tccacacggg ggtctggtct gcggcgcggc
    15241 gcacggtgac gggggcgctc gggtcgccgc tggtgtcgac cagtgctggg aggccgacgc
    15301 cgagggcgtc cgagagacgc agaagggtgc cgatggtggg gttggtgctt ccctgctcga
    15361 cgttgacgag cagtctgcgg ctgacgccgg cccgttgggc gagttggtcg agggtccagc
    15421 ccaggtcggt gcgggcaccc cggactcgtc gcccgatggc catcccgatg gctgaggcgg
    15481 tggcgggctc ggccgcacga ggatccttca tgacgcgcac tatagtgcac tctttggtgc
    15541 atggtaggcc ggggccagtg ccctgcaaga acctcgagcg cagggccttg gtgaagtcgg
    15601 gcttgaatgg gcgcacccgg cgaggcgaag gctgcgagca acagtgacac gatcaggggc
    15661 atgcccctga gactcatcgg ctactggcgc aacgaagatc acccggacta ccccgaaccg
    15721 agtgatctca tcgaccgaga ctggccggac caagatcgcg acgacatcgc cagctacctt
    15781 cgcggcggca tcttcttccg gggattcatg ggtctgtccc cttgccgaat ctgtggacaa
    15841 cacaacgggt ccagcgagtt caccgacggt gtcctggtgt ggcccgaagg tttggcccac
    15901 tacctcaccg agcacgccgt gcggcttccg ccctcgatcg agaactacgt ccggcagcag
    15961 atggactacc tggacagtca gaaaccggac atcgcttggt ggacatccgg agcgcccccg
    16021 accacctcta tcgatcaccc gccactggca ccgcttcgcc gcctggcgtg gttcgggaac
    16081 gttcaactgg ttcaagatcc cgaccggcgc cacccacaac ttctcatcgc cggcgacacc
    16141 cttcacctgc acgccgacgg gcccaaggcc cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnggctcgg gtgcttcgtc agcggcggca gcaacggcct caggcagccc
    16321 aactctcaga cgctcaacgc gggacagagc gtcgtcggga tcgagcccga gggagatccc
    16381 gacgtcggcc cagtcctcgg ccgtcaggtc gcgaacctgc caggtgcgcc ccatcttcat
    16441 agagctccgc gcagcttcac ctttgctgag atacggggcg tatgacgcaa cgtcgtagag
    16501 gggtgcgagg gcgacccgag acccgcgcag caggagggag tagttcttcg cgtgggcgtc
    16561 ggtgccgccg accagaacgt tgaacgcgag ggcgtcgaag aagctgaaac ggacgagatc
    16621 gcggtcgggg agtgccaacg tggcgaagag cgcggccgca ctcttgacgc tcgggccccc
    16681 atccgactgg tacttcttcg agggagggta gttcatcgcc tgcagcagat cttcctgatg
    16741 cagacgaagc cacgcgccgt ccggacctct gccccggtcg taccgggtgg agaccagcgt
    16801 cttcgctcca ccgaagtcga ccaactcgac gtgggcggtt cgaagcccga ggtggccggc
    16861 cgcccgaagg cagacgaact cgttcacgtg cagatccgcg aagcgcgtct ccgcacgcgt
    16921 tggcttgagg atgtgggtcg tcggggtcga gtcctcaggg acgccccagt caccatcatc
    16981 gaggcgatgc agcgccacct tgttctggac gccggcgagg ctccagcggc ccgcgcctgc
    17041 ccccacttcc cagccggcat cgcggacggt cagccctacg agaagatcgt tgacctcgcg
    17101 gtgggagagc cgacggactt tcccggttcg ctgcgctgca tcaggtgggt cgacatcagc
    17161 ggggagaacc tgaacggctc ctgcggcatc gcgccccacg tggcgaagga gggcgaacgg
    17221 gtttcgtggc gaaacttcga acctcctgcc ccactcctct cggacgccga ggttgtcggg
    17281 gagcaggccc tcaagccatg ccgcgacgac gcggttgctg tgccgcgagc cactgagtgg
    17341 catcgacagg gagagcggcg tcgggacacg acgcgtcagg tagaggtcgt cgtagctgaa
    17401 gctcagcgcc ccacccgagg cctgggccag gcgaccggcg aggatgccgt cgaggtagac
    17461 gttgaggtgg tccgtcatgt gcgttccgcg aagaagccgg cgaaggggtc cacgtctgtc
    17521 cctgtctctg ggggcggtgc cgagaccagt tcgagggcaa ggtcgagtgc cgtgaggacg
    17581 tccagcacct tgcccagctc gacggccggg ttcttgcccg actcgaagcc ggacagccac
    17641 tggcgggata ccccggcccg ttgagcaagc atcgcttgcg tgaggcgagc ctcgcggcgc
    17701 cggtgccgaa ctgccgcccc gaggtctcgg accgtggtga gcat
//
LOCUS       DAORPW010000040        17621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_602_length_17621_cov_790.576056, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000040 DAORPW010000000
VERSION     DAORPW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 17621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17621
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_602_length_17621_cov_790.576056"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PLB86_05210"
     CDS             complement(<1..1213)
                     /locus_tag="PLB86_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC64566.1"
                     /translation="MTTNGDSGPTRTLLTGRLVTGGTTTPEGAVAIEDGTIAYAGPLA
                     DLPPNWSAVAPDPRVPTGATLVPGLVDSHLHGGAGGEFGVDAEAARTAIAEHRAHGST
                     TLIGSLVSAGASDLITATQTLGALVASGDLAGIHLEGPFIAAARKGAHDPDALRDGDP
                     GLVDALAEAAAAVGAPGAIRHMTFAPDRAGADLLPAALHRHGIRVNVGHTEADATLTF
                     DVLGRAAATAGAPSLVTHIFNAMPPIRGRSPGTAAGAIRAALRGDAVLEVVADGVHLS
                     PDTVRMLFEVVGPGSINLISDAMAATGLPDGNYTLGGLPVVVADGTARLTSSGAIAGS
                     VAHVADCLRWAVQTAGIDLADAVLAATGTPAKAFGLDVGHLVTGGVADVLVLDADLRP
                     AFVVAGGQASPA"
     gene            complement(1210..1965)
                     /locus_tag="PLB86_05215"
     CDS             complement(1210..1965)
                     /locus_tag="PLB86_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRC64567.1"
                     /translation="MTAVTLPDQILEGPVPKHRQLRDVLARLAVPGEAIPSERDLTAT
                     YGVSRATVRKAIDTLVYEGLLQRTHGLGTFAIRPRLGTQLHLASFTQDMHRRGMVPST
                     RLVSIRREVPPDPIARALGLGPLDRAWSLRRLRLADGQAIALEDAWYPVSLLPDLDHH
                     DLESSLYALFQNQYGLTIDGAEQTLWGEAATGDVARLIACPEHTPLLVFRRVASAGDR
                     PVEYVLSWYRGDRYEVSMSLGSEAAASRAMVPS"
     assembly_gap    2058..2157
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2198..2683)
                     /locus_tag="PLB86_05220"
     CDS             complement(2198..2683)
                     /locus_tag="PLB86_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRC64568.1"
                     /translation="MTAILSPCSGRVIALDEVPDPVFAGRIVGPGVAIDPDRSAATVV
                     APISGRLVKLHPHAFVIAGADVAVLVHLGIDTVQLGGAGFTLHATERTDIAAGDPIVT
                     WDPADIEAGGRSPVVPIILLDVPGETPVDAVCGPDAVIGEHLLTWPADSDPDGATQRR
                     E"
     gene            complement(2680..2952)
                     /locus_tag="PLB86_05225"
     CDS             complement(2680..2952)
                     /locus_tag="PLB86_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose/sucrose transporter subunit IIB"
                     /protein_id="HRC64569.1"
                     /translation="MHAEPHSDPTKGTQMSKAARILSGLGGADNIVEIEACITRLRTE
                     VEDGGLVDQAALKAAGAHGVIVTGNIVQVVVGPEADNLAEDIQDLM"
     gene            complement(2975..3178)
                     /locus_tag="PLB86_05230"
     CDS             complement(2975..3178)
                     /locus_tag="PLB86_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64570.1"
                     /translation="MTATAMAREIAEQPEAVRRTLAAHLSRTDEIAALAAGRRVLFVS
                     RPSRVAQGHPDRIRPLTDPIDPH"
     gene            3413..4654
                     /locus_tag="PLB86_05235"
     CDS             3413..4654
                     /locus_tag="PLB86_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRC64571.1"
                     /translation="MNAQAAARQTSGKRFDLAPLQKFGRSLMLPIAALPAAALLLRIG
                     QPDLLGKDGLGWTQVAAVLGAAGDAIFANLPLLFAVGVAIGMAKKADGSTALAGVVGY
                     LVFKGVADAISPFVLGAAAAGEKQKLINYGVLGGIVMGITAAKLWERYHRISLPPYLA
                     FFGGRRFVPIVTALAAIILAVLLGVIYPAFNSGLTALGNWVVANTIIGGLVYGTLNRL
                     LIPLGLHHILNSVPWFVFGEYTAADGQVYHGDIARFLHGDPAAGPFMTGFFPIMMFAL
                     PAAAFAIWRAAKPSQRKAVGGIMLSGALTSFLTGVTEPLEFSFMFVAWPLYVLHSVLT
                     GTSLAVANALGIHDGFGFSAGLFDFALNWNIATKPALLIVLGLIYAVVYYVLFTWVIR
                     KWNLRTPGREDDDSTTADTSA"
     assembly_gap    4843..4942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4943..7849
                     /locus_tag="PLB86_05240"
     CDS             <4943..7849
                     /locus_tag="PLB86_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRC64572.1"
                     /translation="TSAALLAAVVLALVPGTSVGLAASTGLAASPANPTAAGLGADGT
                     PGPAIEETLSVPVGPEPDGTAVALDVTVFRPGGLGQHSEPWSAVLLAHGFGGSKADLI
                     ERGRDLASRGYLVLGYSARGFGASGGRIHLNDPAYEIADARALVSLLATRSDVLLDAP
                     GDPHVGVVGASYGGALAIMLGATDPRVDSVVGVVAWNDLADAFFPQFAVVGPRSPLGP
                     AQVDRSPEPGPLKALWASRFFGSALASAGSPGAGAGAGAGAVTGSGGAGAVCGRFDPT
                     VCRLFLEATVTGRPSPELLTVLRAHSPRPLLSGLRAPTMLVQGMADSLFGIEQAAATA
                     RAIAVQVPRLAIRWIDGGHDGLSSTAAADEQALRDWLADTLRGTTLLAGAGQFIYAAP
                     IPRRRTVAPLFTTPDSPPTATWTAVPLAPLVGASGGATSDPQRIVTPPGGQPASVTAI
                     PSLGGLGVGAAAYQLAALPGQSAAFDSPPFAQQTAIVGAPRLTLTVTSTGPETVLFLS
                     LWQVTAGQATLPRRLVAPVRVLTTPGQPTSVDVALAPATWTVEAGSSLRVLITSTDSA
                     YAAPREARVDLVAVAGGELRLPHVDGYLLAAESDLDTESVGVATAIALLLAAFGVLAW
                     RERRRRRLLPDRDDLADVPLAVEHLVKTYADGHRAVGDVSWRAERGQVVGLLGPNGAG
                     KTTTLRMAMGLITPDSGAVYLGGRAVRPGAPALRGVGALVEGPGFLPHLTGRANLHAY
                     WAATGRPIEEARLDEALDVAALGGAVDRPVRSYSHGMRQRLGIAQAMLGLPDLLVLDE
                     PTNGLDPPQIAAMRPILQRYAAAGRTVVVSSHLLAEVEQTCSHVVVMHAGRVVTAGPV
                     ADLIDSSDTTVVHLDPAATAETIAAVADRLRSVAGILEVEIVEDEGDSRLVVTAGMPR
                     PDVVRALTEVGADVVGLSSRKHLEEVFLRVIAAAQTAGEPTGSVTERLRQVRAR"
     gene            7846..8748
                     /locus_tag="PLB86_05245"
     CDS             7846..8748
                     /locus_tag="PLB86_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC64573.1"
                     /translation="MTTDPVAKQPSAADPSATEPSATEPSGRHTRGALSFGAEVRRQL
                     GRRRTLWSFVIMLALPVILIGAFALGRTSGPPGGSRLSDLAQQGSANFAIFALAAASD
                     FLLVVLAALFAGDAVPAEASWSTLRYLLIAPVPRARLLASKWAVAIVSLGVAIVVYLG
                     WGLLVGGLAYGWAPFTNPAGGVLGWGELLPRLAIAAAYLFVTLLQVAGIAFVLGVRTD
                     APLGAVGVAVLVAIVSSILGQIDSLGDLRNGLPLHYIRAWQDLLTPQVDWTAMGHGVL
                     WSVLYTLLTVGLAFAVFRRKDVLS"
     gene            8933..9910
                     /locus_tag="PLB86_05250"
     CDS             8933..9910
                     /locus_tag="PLB86_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRC64574.1"
                     /translation="MLDPKAAGEIARAFGLGANARLEGPVAHGRKGEVWRLVTDEGRY
                     AVKALFGTVFRERAERDAAYQDVVRDTGVPMPAVLRTLSGNVLADVGGPPPNATVRVY
                     EWVDILEPRRDLDPREIGRLLARVHNVEAPAAGTVDPWYREPVGPQVWGELVEALRAE
                     DAPFATDLAALVPQLVALEQTFEVPADVRTCHCDLWADNVRATRDGGIVLLDWESAGP
                     ESPSHELGMVVFEYGLGDPARMLDLYAAYLIAGGPGRISRAGDLTMLGATSEHLAEEG
                     CRRWLSATDEGDRELAAAWVEWLLHEPITPATVADVLDAVAGLGGSRGA"
     gene            complement(10055..11308)
                     /locus_tag="PLB86_05255"
     CDS             complement(10055..11308)
                     /locus_tag="PLB86_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC64575.1"
                     /translation="MSVTPRAPRTPRTPPSAVPAVTSLALEQDVFERLRGVVPRVAAE
                     AVETIMREVPSYARPFSDAMGARIRTAVELALVNFLELARVGSGTDPAEPIAPSTAAA
                     YELGRGEARSGRSMDALQAAYRVGARVCWRGLSREAVATGVPAATLGAFAELVFAYID
                     ALSSASISGHADELATAGRARERYRDRLAALLVGGGEEADLVAAARRADWHPPRSLTA
                     VLLPEGQLRAVLGLLDARTLAMSTEPAGVDDEDAGVLLVPDATGRDRERLVRQLEGRG
                     AIIGPERPWLQVHRSFQRVLRAREFLPQPNSRGTLDTDDLLAELLLSADPEALADLRA
                     RALAPLAAVRPTVRLRLEETLRSWLLHHGRRELIAAHLFVHPQTVRYRMAQLRELFGD
                     RLDDPEEVLRLTMALALQPTRPPTP"
     gene            11404..12498
                     /locus_tag="PLB86_05260"
     CDS             11404..12498
                     /locus_tag="PLB86_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HRC64576.1"
                     /translation="MSATVTSPRGRSLRQRAWRAAEMLTGALMPADTLDVINPLRPGA
                     DPLAGRIERIHLETADAATVFIKPGADWSGHIPGQYLRFGIDIGGVRQWRTYSLTSRL
                     DRPDGLLTITTKAIPDGLVSNHVVRDLRPGTLVHIGQAEGDFVLPTARPDKVLFLTGG
                     SGITPVMGMLRNHIAELPDVVMIHSAPTARDVIFATELRELAATGLMRLVELHTDTDG
                     MLDLAVDLDRLVPDWREREVWACGPAGMLDACETHWSTAELRERLHVERFRTHLHAAG
                     EGGTVTFTKTGTIVEADGGRPILEVAEEAGMLMPHGCRMGICYQCVLPLRDGAVRDLR
                     TGEVTYAAPGDGVAIQTCVSAAAGACEIEH"
     gene            12510..13853
                     /locus_tag="PLB86_05265"
     CDS             12510..13853
                     /locus_tag="PLB86_05265"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRC64577.1"
                     /translation="MTLVAEPHPVATDPTMPSERSGQRAGSAFAPPAPPVDARVNPHL
                     RLVDGKERVLHPKPSGRPDPTAHLSAADIEALGRELDAIRQTVMDSRGADDAAYIRKV
                     IAFNRYLELTSRAILLFSLFPPAWLVGTAGLSVAKIIDNMELGHNILHGQWDWMRDPK
                     IHSTTWEWDHVTPAAQWKESHNETHHTYTNVLGRDNDLGYGIMRVDADQPWHLANLGQ
                     PLYSFINALFFEWGIASYDLKLHDVITGKMPREESKPRVDAMLTKMRRQVLKDYLIHP
                     LLSGPSFFPTMGANFVATIIRNLWSNGVILCGHFPFGVDTFAYDSIEGETRGEWYVRQ
                     MLGSANISGGWLVHFMSGNLSHQIEHHLFPDLPSNRYAQVAPQVREICQRYGLHYVTG
                     SYPRQLASAWAKVFVYALPDKEPDRSRGQIVASAVKDAFVAGKAKRAAKRAAKLA"
     gene            14003..>15200
                     /locus_tag="PLB86_05270"
     CDS             14003..>15200
                     /locus_tag="PLB86_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRC64578.1"
                     /translation="MARIPDYLDFDLELTDAGEGGYTARVLASPKGEASADFRMPFLG
                     VDLENVILKLGRTRSGVRALGSPQHDLARTFGGQLYDTVFSGEVGTCFRRSLDEADEQ
                     GKGLRIKLRLTDVPSLADVPWEYLYAAGLGRFLVLSESTPIIRYLDLPREVLPLTVTP
                     PLRILLAVCSPTNLTQLDTQAEVARVAESLRDLVERGLVIVDPLPNATLTALRQALRA
                     TDYHVLHFIGHGGFDPVAGDGVLAFEDADHLGHLVSGQDLGTLLHDMRTLRLAVLNAC
                     EGARQSPTDPFSGVAQCLVRQGLPAVVAMQFEITDTAATSLSSEFYAALADGLPIDAA
                     LAHARMSVFAADNDVEWGTPVLYLRARDGRIFDVATQDAAPPTPAPMPTPTPVATPAP
                     TPAPAPP"
     assembly_gap    15201..15300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15301..15497
                     /locus_tag="PLB86_05275"
     CDS             <15301..15497
                     /locus_tag="PLB86_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64579.1"
                     /translation="YADTSGDTRADTRARSTITHPDPLPDLDHSTDSGADARTDIRNT
                     PADARADATYPANGGGRHTC"
     gene            15532..16302
                     /locus_tag="PLB86_05280"
     CDS             15532..16302
                     /locus_tag="PLB86_05280"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRC64580.1"
                     /translation="MAPAVVAAVIVTLGLVAAFLLRPDGGAATNGPGPAPKEFNGFVA
                     MRGTPVIDGQANEWAGAVPYVSDVLVAGKATKIKATWSLMWDQQAFYVVADVADPNLM
                     PADQTAPDQQWRGDSVSFELGADPRGLAVEAGLRATDAHYIVGLTPTTPARTKIVINR
                     ANVTKNTIDAGRFHGESDAVVALIPGGYRVEARLPWAVTGVAPVGVQTGQVFGINLNA
                     SDGGSSPKEFAAMYSSNRGRTSANQVRPAAWQVVQLQG"
     gene            16422..16640
                     /locus_tag="PLB86_05285"
     CDS             16422..16640
                     /locus_tag="PLB86_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64581.1"
                     /translation="MARYDITTATLGTLLADPDVVAIMEKHAPGITTNPMIGMAAGMP
                     AAQAVAMAGGMIGGDAVSAITDEVTSLN"
     gene            16888..>17621
                     /locus_tag="PLB86_05290"
     CDS             16888..>17621
                     /locus_tag="PLB86_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HRC64582.1"
                     /translation="MAAKATTPAIELAVGERTIRLSNPDRVYFPEHGWTKLDLANYYL
                     AVGDGIVRALRERPCMLHRFPEGLAGEKVHQKRIPHGAPPWLQTVRLHFPRWNRTADE
                     LCVTELGSVIWAVQMSTVEFHPWNVRRADVDRPDEWRIDLDPMPEAGFDRVRVVAGVV
                     REVLDELGIVGFPKTSGGNGLHVYVRIVPEWEFGDVRRAALAFAREVERRLPGDATTT
                     WWRKDRDPRHVFIDYNQNARDHTIAAA"
BASE COUNT         2472 a         6230 c         5934 g         2685 t
ORIGIN      
        1 gcgcgggact cgcctgaccg ccggcaacca cgaaagccgg gcgcaggtcg gcgtcgagca
       61 cgagcacgtc ggcgacccca ccggtgacca ggtgtccgac gtccagaccg aaggccttgg
      121 caggcgtgcc ggtggcggcc aggacggcgt cggccaggtc gatcccggcg gtctgcacgg
      181 cccagcgcag gcaatcggcg acgtgcgcga cgctgccggc gatggctccg ctgcttgtga
      241 gccgggcggt gccgtccgcc acgacgaccg gcaggccgcc gagcgtgtag ttgccgtccg
      301 gcagcccggt ggccgccatc gcgtcgctga tgaggttgat cgacccaggg ccgaccacct
      361 cgaagagcat ccgcaccgtg tcgggggaca ggtggacgcc gtcggcgacc acctcaagaa
      421 cggcgtcacc gcgcagggcc gcgcggatcg ccccggcagc cgtgccgggc gagcggccac
      481 ggatcggcgg catcgcgttg aagatgtggg tgacgagcga cggggctccc gcggtggctg
      541 cggcccgacc caggacgtcg aaggtcagcg tcgcgtccgc ctcggtgtgc cccacattga
      601 cccgtatgcc gtgtcggtgc agcgccgcgg gcagcaggtc ggcgccggct cggtccggcg
      661 cgaaggtcat gtggcggatc gccccaggag cgccgaccgc tgcggcggcc tcggccaggg
      721 cgtcgaccag cccgggatcg ccgtcccgca gggcatccgg atcgtgagcc cccttgcggg
      781 ctgcggcgat gaagggacct tcgaggtgga tgccggccag gtcgccggag gcgacgagag
      841 cgccgagggt ctgtgtggca gtgatcaggt cggaggcccc ggccgagacc aggctcccga
      901 tgagcgtggt ggacccgtgc gcccggtgtt cggcgatggc cgtgcgggca gcctcggcgt
      961 cgacgccgaa ctcgccgcca gctccgccgt gcaggtgcga gtcgacgagc ccgggaacga
     1021 gcgtggcgcc ggtcggcacc cgcggatcgg gcgcgactgc ggaccagttc ggtggcaggt
     1081 cggccagcgg gccggcatac gcgatggtcc cgtcctcgat cgcgacggcg ccctcaggcg
     1141 tggttgtgcc gccggtgacg agccgaccgg tgaggagggt gcgcgtcggg ccggagtcgc
     1201 cgttggtcgt catgacggga ccatcgcgcg gctggccgcc gcctcgctgc cgagggacat
     1261 gctgacctcg taacgatcgc cgcgatacca ggacaggacg tattcgaccg ggcggtcacc
     1321 ggcggaggcg acccggcgga agacgagcag cggagtgtgc tcggggcagg cgatcaggcg
     1381 ggcgacatcg ccggttgccg cttcgcccca gagggtctgc tcggccccgt cgatggtcag
     1441 cccgtactgg ttctggaaca gcgcgtagag cgagctctcg aggtcgtggt ggtccaggtc
     1501 cggcaggagg ctgacgggat accacgcgtc ctcaagcgcg atggcctgtc cgtcggcgag
     1561 ccgcaaccgg cgcagtgacc aggcccggtc gaggggtccg agccccaggg cgcgggcgat
     1621 cgggtccggg ggcacctcac gacggatcga gaccaaccgg gtcgagggca ccatgccgcg
     1681 gcgatgcatg tcctgagtga aagacgccag gtgcagttgg gtgccgaggc gcggccggat
     1741 ggcgaaggtt cccaacccgt gggtgcgttg gagcaacccc tcatagacga gggtgtcgat
     1801 cgccttgcgg accgtggctc gcgagacgcc gtaggtggcg gtcaggtcgc gctccgaagg
     1861 gatggcctcg ccggggacgg cgaggcgggc caggacgtcg cgaagctgcc gatgctttgg
     1921 caccggtccc tccaagatct gatccggcag cgtgacggct gtcatggtta ggtccctccc
     1981 cacgtggaca aggtggtctt cattcgcagc ccgggcggca ccgcccgccc gaacgcgaga
     2041 gtcgaatcga gagtcgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga
     2161 gagtcgaatc gagagtcgag acgacgtgga tacggcctca ttctctacgc tgcgtggccc
     2221 cgtccgggtc gctgtccgcg ggccacgtga gcaagtgttc gccgatgacg gcgtcgggtc
     2281 cgcacacggc gtcgaccggg gtctcaccgg ggacgtcgag caggatgatc ggcacgaccg
     2341 ggctgcgtcc gccggcctcg atatccgccg gatcccacgt gacgatcggg tcgccggctg
     2401 cgatgtcggt gcgctcggtg gcgtgcaggg tgaaccccgc gccaccgagt tggacggtgt
     2461 cgatgcccag gtggaccagg acggcgacat cggctccggc gatgacgaac gcgtgcgggt
     2521 gcagcttgac caggcgcccg gagatcgggg ccacgacggt ggccgcgctg cggtccgggt
     2581 cgatggccac gcccggcccg acgatccggc cggcgaagac ggggtcgggg acctcgtcga
     2641 gggcgatgac ccgtccgctg caggggctga ggatcgcggt cacatgaggt cctggatgtc
     2701 ctcggcgagg ttgtccgcct cggggccgac gacgacctgg acgatgttgc cggtgacgat
     2761 gacaccgtgg gcgccggccg ccttgagggc cgcctggtcg acgagcccgc cgtcttcgac
     2821 ctcggtccga agccgcgtga tgcaggcctc gatctcgacg atgttgtcgg cgccaccgag
     2881 gccggagagg atgcgtgctg ccttgctcat ttgagttcct ttcgtgggat cactgtgcgg
     2941 ttctgcgtgg atcactgtgc ggttctacgg gatatcagtg cggatctatc gggtcggtca
     3001 gtggacgaat tcggtctggg tgaccttgcg caacccgcga ggggcgggag acgaacagga
     3061 cccggcggcc ggcggcgagg gccgcgatct cgtcggtgcg agacaggtgc gcggccaggg
     3121 tccggcgaac cgcctcgggc tgctcggcga tctctcgggc catcgcggtg gctgtcattc
     3181 ggagcgttct cctcggggct tgacaaacgg tgggagcctg ggtcatgctt actggtacgt
     3241 accagagcgt atcagtacgg accacgaaca gcaagccccg cagaagcccc gcagaagccc
     3301 cgcaagtgca ctgaagaacc accgaagtag caccgccgta gttcctcagg agcacggcga
     3361 gaagcccccg gctccaccct cccgatcgct cgtccgtccc gaaaggactt tgatgaacgc
     3421 ccaagccgcc gcccgccaga cgagtgggaa acggttcgat cttgcgccgc tgcagaagtt
     3481 cggtcgcagc ctcatgctcc ccatcgccgc cctccccgcg gccgccctcc tcctgcgcat
     3541 cggccagccc gacctgctcg gcaaggacgg cctcggttgg acccaggtgg ccgccgtcct
     3601 cggcgcagcc ggtgacgcga tcttcgccaa cctgccgctg ctgttcgccg tcggtgtcgc
     3661 catcggcatg gccaagaagg ccgacggctc gaccgccctc gccggtgtcg tgggttacct
     3721 cgtcttcaag ggggtggccg acgcgatctc accgttcgtc ctcggagctg ccgccgccgg
     3781 cgagaagcag aaactcatca actacggcgt cctcggcggc atcgtcatgg gcatcaccgc
     3841 cgccaaactc tgggagcgct accaccggat cagcctcccg ccctacctcg ccttcttcgg
     3901 cgggcggcgc ttcgtcccga tcgtcacggc cctggccgcg atcatcctgg ccgtcctgct
     3961 cggcgtgatc tacccggcct tcaacagcgg tctgaccgcg ctgggcaact gggtcgtggc
     4021 caacaccatc atcggtggcc tcgtctacgg cacgctcaac cgcctgctca tcccgctcgg
     4081 cctgcaccac atcctcaact cggtgccgtg gttcgtcttc ggtgagtaca ccgccgccga
     4141 tggccaggtt taccacggtg acatcgcccg cttcctccac ggcgacccgg ctgcaggccc
     4201 cttcatgacc ggcttcttcc ccatcatgat gttcgccctc ccggccgcgg ccttcgcgat
     4261 ctggcgcgcg gccaagccct cccagcgcaa ggccgtcggc ggcatcatgc tctcgggcgc
     4321 cctgacgtcg ttcctcaccg gggtgaccga gccgctggag ttctcgttca tgttcgtcgc
     4381 ctggccgctc tacgtcctgc actcggtcct caccggcacg tcgcttgcag tcgccaacgc
     4441 cttgggcatc cacgacggat tcggcttctc cgcaggcctg ttcgacttcg cgctcaactg
     4501 gaatatcgcc accaaaccgg ccctgctcat cgtgctgggt ctgatctacg cggtggtcta
     4561 ctacgtcctc ttcacctggg tcatccgcaa gtggaacctg cggacccctg gccgcgagga
     4621 cgacgactcg acgacggccg acaccagcgc ctgatcgtca cagacacccg cttgcggcgg
     4681 gtcacgcagc agccggtccg agagcccact cggaccggct gtttcgcgtt gacctcacct
     4741 tccggcgggg ccgggttacg ctccggctca tgtatgccgt ccgccgcacc tccgcggccc
     4801 tgctcgccgc cgtggtgctg gcgctggtgc cggggacgtc ggnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnacctccgc ggccctgctc gccgccgtgg tgctggcgct
     4981 ggtgccgggg acgtcggtcg ggctcgcggc gtcgaccggg ctcgcggcat cgcccgcgaa
     5041 cccgacggct gccgggctgg gcgcggacgg aacaccggga ccggccatcg aagagacgct
     5101 gagcgtcccg gtgggcccgg agcccgacgg gacggcggtg gcgctggacg tgaccgtctt
     5161 ccgtccaggc gggctcggcc agcacagcga accctggtcg gcggtcctgc tggcccacgg
     5221 cttcggtggc agcaaggccg acctgatcga gcgtggccgt gaccttgcct cccggggcta
     5281 tctcgtgctc ggctactccg ccaggggttt cggcgcgtcg ggaggccgga tccacctcaa
     5341 cgacccggca tacgagatcg ccgatgcccg cgccctcgtg agtctgctcg ccacccgcag
     5401 tgacgtgctc ctggatgcgc ccggcgaccc gcacgtcggc gtggtggggg cgtcctacgg
     5461 tggggcgctc gcgatcatgc tcggcgcgac cgacccgcgc gtggacagcg tcgtcggagt
     5521 cgttgcgtgg aacgacctgg ccgacgcctt cttcccgcag ttcgcggtcg tcgggccgcg
     5581 gagtccgttg gggccggctc aggtggatcg gtctcccgag ccgggaccgc tcaaggcgct
     5641 ctgggcgtcc cgattcttcg gctcggcact cgccagtgct ggttcgccgg gtgctggtgc
     5701 tggtgcaggt gcaggtgcag tcacgggctc gggcggcgcg ggcgccgtgt gtgggcgatt
     5761 cgaccccacc gtgtgtcggc tgttcctgga ggcgacggtg actgggcgac cgagccccga
     5821 gttgttgacg gtgttgcgcg cccacagccc ccgtccgctg ctttccggcc tgcgcgcgcc
     5881 gaccatgctc gtccagggga tggccgactc gctgttcggc atcgagcagg ccgccgcgac
     5941 tgcgcgagcg atcgcggtcc aggtgccgcg gctggcgatc cggtggatcg acggtggtca
     6001 cgacgggctg tcgagcaccg ccgcagccga cgagcaggcg ttgcgcgatt ggctggccga
     6061 caccctccgc ggcaccacgc ttctggccgg cgcgggccag ttcatctacg ccgcaccgat
     6121 tccgcggcga cggacggtgg ctccgctctt caccacgccc gactctccgc ccacggcgac
     6181 ctggacagcc gttccccttg cgccccttgt cggcgcctcg gggggcgcga cgtccgaccc
     6241 gcagcggatc gtcacgccac cgggtggtca gccggcgtcg gtgaccgcga ttccgtcgct
     6301 cggggggctg ggagtgggcg cggcggcata ccaactggca gctctcccag ggcagtccgc
     6361 cgccttcgac agtcctccct tcgcgcagca gaccgccatc gtcggtgcgc cgcgactgac
     6421 cctcacggtg acctcgaccg ggcccgagac cgtgctcttc ctctcgctgt ggcaagtgac
     6481 ggccggccag gccaccctgc cgcggcggct ggtcgcgccc gtgcgggtgc tcaccacgcc
     6541 gggacagccg acctcggtcg acgtcgcgct cgctccggcc acctggacgg tcgaggcggg
     6601 cagctcgctt cgggtgttga tcacctcgac cgactcggcg tatgccgccc cccgcgaggc
     6661 tcgcgtcgac ctggtggcgg tcgccggcgg ggagcttcga ctgccgcatg tcgacggcta
     6721 cctccttgct gcggagagcg acctggacac cgagtcggtc ggggtcgcga cggcaatcgc
     6781 gctgctgctg gccgctttcg gggtgttggc ctggcgcgag cggcgccgac gacggttgct
     6841 gcccgaccgg gacgacctcg ccgacgtgcc gttggcggtt gagcacctcg tgaagaccta
     6901 cgccgacggt caccgcgccg tcggcgatgt gtcgtggcgg gccgaacgcg gccaggtcgt
     6961 cgggctgctg ggacccaacg gggcggggaa gacgacgacg ctgcgcatgg cgatggggct
     7021 catcaccccc gactctggcg cggtctatct cggcgggcgg gcagtgcgcc caggggcacc
     7081 ggcgctgcgc ggcgtcgggg cgctggtcga ggggccgggc ttcctgccgc atctcaccgg
     7141 acgggccaac ttgcacgcgt attgggcggc caccggtcgc ccgatcgagg aggcccggct
     7201 cgacgaggcc ctcgacgtgg cggcgctcgg cggcgcggtc gaccggccgg tgcgctccta
     7261 cagccacggc atgcgccagc ggctcggcat cgcccaggcc atgctcgggc tgcccgacct
     7321 gctcgtcctc gacgagccca ccaacgggct cgaccctccg cagatcgccg cgatgcgccc
     7381 gatcctgcag cggtatgccg ccgccggccg caccgtcgtc gtctccagcc acctgctggc
     7441 cgaggtggag cagacctgct cgcatgtcgt cgtcatgcac gccgggcggg tggtcaccgc
     7501 tgggccggtc gccgatctca tcgactcctc cgacacgacc gtcgtccacc tcgacccggc
     7561 cgcgacggcg gaaaccattg cggctgttgc tgatcggctc cggtcggtcg ccgggatcct
     7621 cgaggtcgag atcgtcgagg acgagggtga ctcgcggctg gtcgtcaccg cgggtatgcc
     7681 gcgccccgac gtggtccggg cgctcaccga ggtcggtgcc gacgtcgtcg ggctgagcag
     7741 ccgcaaacat ctcgaggagg tcttcttgcg ggtcatcgcc gccgcccaga ccgcaggaga
     7801 gccgaccgga tcggtcaccg aacgactgcg acaggtgcgg gcccgatgac cacggacccg
     7861 gtcgccaagc agccgagcgc cgcggacccg agcgcgacgg agccgagcgc cacggagccg
     7921 agcgggcggc atacccgagg ggcgttgtcc ttcggcgcgg aggtgcgccg acaactgggc
     7981 cgccgtcgca cgctgtggag tttcgtcatc atgcttgcgc tgccggtgat cctcattggc
     8041 gcgttcgcgc tcggccgcac gagtggcccc ccgggcggct cgcggctgtc ggacctcgcc
     8101 cagcagggca gcgctaactt cgcgatcttc gccctggcgg ccgcctccga cttcctcctc
     8161 gtcgtcctcg cggccctctt cgcgggcgac gcggtgccgg ctgaggcgtc ctggtcaacc
     8221 ctgcgctacc tcctcatcgc gcccgtcccg cgggctcggc tgctggcgag caaatgggcc
     8281 gtcgcgatcg tcagcctcgg ggtcgccatc gtcgtctatc tcggttgggg gctgcttgtt
     8341 ggcggtcttg cctatgggtg ggctcccttc accaatccgg ctgggggagt gttgggttgg
     8401 ggcgagttgt tgccccggct ggcgattgcg gcggcctatc tcttcgtgac cctgctgcag
     8461 gtggcgggga tcgcgttcgt gctcggtgtg cggaccgacg cgccgctggg tgccgtcggg
     8521 gtggccgtgc tggtcgcgat cgtctcgtcg atcctcggcc agatcgacag cctgggcgac
     8581 ctgcgcaacg gcctcccgct gcactacatc cgcgcatggc aggacctgtt gacgccccag
     8641 gtggactgga ccgcgatggg gcacggggtg ctctggtcgg tgctctacac gcttctcacc
     8701 gtcgggttgg ccttcgccgt gttccgccgc aaggacgtcc tcagctgagg cgaccttctc
     8761 gaccgggctc gcggcggcgg cccgccgagc cccagcgagc cctgaccacg attgtcagcg
     8821 tcgtgacgga cgtctcgtgg aaggcggatc acacatcgag cgttccctgg gggtgacctg
     8881 tcacgctgtt actgacgggt aacactgaaa tcaacgaccc tggggggcga cgatgctgga
     8941 tccgaaggcg gcgggggaga tcgcccgcgc attcgggctg ggcgcgaacg cccgcctcga
     9001 aggcccggtc gcccatggcc gcaagggcga agtctggcgc ttggtcaccg atgagggtcg
     9061 gtatgccgtg aaggcccttt tcggcacggt gttccgcgag cgggcggagc gggacgcggc
     9121 ataccaggac gtcgttcgcg atacgggcgt accgatgccc gcggtcctgc gcaccctctc
     9181 gggcaacgtc ttggccgacg tcggtgggcc gccccccaac gcgaccgtca gggtctacga
     9241 atgggtcgac atcctcgagc cgcgccgtga ccttgatccg cgcgagatcg ggcggctgct
     9301 cgcccgcgtg cacaacgtcg aggcgcccgc tgccggaact gtggatccct ggtaccgcga
     9361 gccggtcggc cctcaggtct ggggcgagct cgtcgaggcc ttgcgtgcgg aggacgcgcc
     9421 cttcgccacc gacctcgccg cgctggtgcc ccaacttgtc gctcttgagc agactttcga
     9481 ggtgcccgcg gacgtccgga cctgccactg cgacctctgg gccgacaacg tccgcgcgac
     9541 gcgtgacggc gggatcgtcc tgctcgactg ggagagtgcc ggccccgaga gcccgtcgca
     9601 cgagttgggg atggtcgtgt tcgaatacgg ccttggcgac ccggcccgga tgctcgacct
     9661 gtatgccgcc tacctcatcg ccggagggcc cggccggatc tcgcgcgccg gtgacctgac
     9721 catgttgggg gcgacgagcg agcacctggc cgaggaaggc tgccgccgct ggctctcggc
     9781 gaccgatgag ggcgaccgcg agctcgccgc cgcgtgggtc gaatggctgc tgcacgagcc
     9841 gatcacgccg gccaccgtgg ccgacgtcct cgacgccgtg gccggcctcg gtgggagccg
     9901 cggggcgtag cctcgactgg ccgcgccgca cggcatacct gatcgcggcg ctgggccggg
     9961 gtcggggccg cgcatgaagc cttctgcggg ggtgtgatta ccccaccggg ggctgcttca
    10021 tcggctgcct cacccccggt ctcacgccgc gcgctcaggg cgtcggtggc cgcgtgggtt
    10081 gcagcgccag cgccatcgtc agccgcagga cctcctcggg gtcatcaagc cggtccccga
    10141 acaactcgcg caactgggcc atgcggtagc gcaccgtctg tgggtggacg aacaggtggg
    10201 cggcgatgag ctcgcgacgg ccgtggtgca gcagccacga ccgcagcgtc tcctccagcc
    10261 gcagccgcac ggtcgggcgc accgcggcga gcggcgccaa ggcccgagcc cgcaagtcgg
    10321 ccagcgcctc agggtcggcg ctcagcagca actcggccag gaggtcgtcg gtgtcgaggg
    10381 ttccgcgcga gttgggctgg gggaggaact cgcgagcgcg caacacccgc tggaacgacc
    10441 gatgtacctg cagccacggt cgctcgggcc cgatgatcgc cccgcgccct tccaactggc
    10501 gcaccagccg ctcccgatcc cgcccggtgg cgtcgggcac gagcaggacg ccggcgtcct
    10561 cgtcgtcgac gccggccggc tcagtcgaca tcgccagggt cctggcgtcg aggagcccga
    10621 gcaccgcgcg cagttgcccc tcgggcagga gcaccgcggt gagcgaccgt ggcgggtgcc
    10681 agtcggcccg ccgagcggcc gcgacgaggt cggcctcctc gccgccaccg acgagcagcg
    10741 cggccaatcg gtcgcgatag cgctcgcgcg cgcgcccggc agtggccagt tcgtcggcat
    10801 gacccgagat gctcgcggac gacagggcgt cgatgtaggc gaacaccaac tcggcaaacg
    10861 cccccagcgt ggccgccggc accccggtcg ccactgcctc ccgcgacaga ccgcgccagc
    10921 agactcgcgc cccgacccgg tatgccgcct gcagcgcgtc catggaccgg ccactgcggg
    10981 cctcgccgcg gcccaactca taggctgcag ccgtgcttgg ggcgatgggt tcggcagggt
    11041 cggtgccgct gccgacccgg gccagctcga ggaaattgac cagggccagc tcgacggcgg
    11101 tacggatgcg cgcacccatg gcgtccgaaa acggccgggc atagctgggc acttcgcgca
    11161 tgatggtctc gacggcctcg gcagccaccc gcgggacgac gcctcggagt ctctcgaaga
    11221 cgtcctgttc gagggccaag gacgtgaccg ccggcaccgc gctggggggc gttcggggag
    11281 tgcgaggggc ccgtggggtg acggacatgg cttcaccgcc ttgtctctcg cgaacaaaag
    11341 ttcagctccg cttcacgtcg agccatcaga aatctagcgc agaaacccgg aacaatgggt
    11401 tctatgtccg ccacggtcac ctcgccgcga ggccgcagtc ttcgacaacg cgcctggcgc
    11461 gccgccgaga tgctcactgg agccctcatg ccggccgaca ctctcgatgt catcaacccc
    11521 ttgcgccccg gcgccgaccc gctcgcgggc cggattgagc ggattcatct cgaaaccgcg
    11581 gacgctgcaa ccgttttcat caaacctgga gccgactggt cggggcacat ccccgggcag
    11641 tacctccgct tcggcatcga catcggtggt gtcaggcagt ggcgcaccta ctcgctgacc
    11701 agccggctcg accgtcccga cggcctgctg acgatcacga ccaaggccat ccccgacggc
    11761 ttggtgagca accacgtcgt ccgtgacctg cgcccaggca cgctggtcca catcggccag
    11821 gccgaaggcg acttcgtcct cccgacggcc cgcccggaca aggtcttgtt cctcaccggc
    11881 ggcagcggca tcacgcccgt catggggatg ctgcgcaacc acatcgccga gttgcccgac
    11941 gtggtgatga tccactccgc gccaaccgcc cgcgacgtca tcttcgcgac cgagctgcgc
    12001 gagctggccg ccaccggcct gatgcgcctg gtcgagttgc acaccgacac cgacggcatg
    12061 ctcgacctcg cggtcgacct cgaccgcctc gtccccgact ggcgtgagcg tgaggtctgg
    12121 gcgtgtggcc cggccggaat gctcgacgcg tgcgagaccc actggtcgac cgccgagctg
    12181 cgcgagcgcc tgcacgtcga gcgcttccgc acccacctgc acgccgctgg cgagggtggc
    12241 accgtcacct tcaccaagac cggaacgatc gtcgaggccg acggcggccg cccgatcctt
    12301 gaggtcgccg aagaggcggg catgctcatg ccgcatggtt gccgcatggg catctgctac
    12361 caatgcgtct tgccgctgcg cgacggcgcg gtccgcgacc tgcgcaccgg cgaggtcacg
    12421 tatgccgccc ccggcgacgg tgtcgccatc caaacctgcg tgtcggctgc cgccggcgcg
    12481 tgcgagatcg agcactgagg agccactgaa tgaccctcgt cgccgagccg caccccgttg
    12541 cgaccgaccc cacgatgccg agcgagcggt ccggccagcg ggccgggtct gccttcgcgc
    12601 cccccgcccc gcctgtcgac gcccgcgtca acccacacct gcgtctggtc gacggcaagg
    12661 agcgggtcct gcaccccaag ccgtccggtc gtcccgaccc gaccgcgcac ctgtcggccg
    12721 ccgacatcga ggccctgggt cgtgagctcg acgccatccg ccagacggtc atggattcgc
    12781 gcggagccga cgacgcggca tacatccgca aggtcattgc gttcaaccgc tacctcgagt
    12841 tgaccagccg cgcgatcctg ctgttctcgc tcttcccgcc ggcctggttg gtcggcaccg
    12901 cgggcctgtc ggtcgccaag atcatcgaca acatggagtt gggccacaac atcctccacg
    12961 gccagtggga ctggatgcgc gaccccaaga tccactcgac gacctgggaa tgggaccacg
    13021 tcacgccggc ggcccagtgg aaggagtcgc acaacgagac tcaccacacc tacaccaacg
    13081 tcctcggccg cgacaacgac ctcggctacg gcatcatgcg cgtcgacgcc gaccagccct
    13141 ggcacctggc caacctcggt cagccgctct acagcttcat caacgccctg ttcttcgagt
    13201 ggggcatcgc cagctacgac ctgaagctgc atgacgtcat caccggcaag atgccccgcg
    13261 aggagtccaa gcctcgggtc gacgccatgc tgaccaagat gcgtcggcag gtcctcaagg
    13321 actacctcat ccacccgctg ctgtccgggc cgtcgttctt cccgaccatg ggcgccaact
    13381 tcgtcgcgac gatcatccgc aacctgtggt ccaacggggt catcctctgt gggcacttcc
    13441 cgttcggcgt cgacaccttc gcctacgact cgatcgaggg cgagacccgc ggcgagtggt
    13501 atgtccgcca gatgctcggt tcggccaaca tctccggtgg ctggctcgtc cacttcatgt
    13561 cgggcaacct cagccaccag atcgagcacc acctgttccc cgacctgccg agcaaccgct
    13621 acgcccaggt cgccccgcag gtgcgtgaga tctgccagcg ctacggcctg cactacgtca
    13681 cgggttccta cccgcgtcag ctcgcctcgg cctgggccaa ggtcttcgtc tacgccctgc
    13741 ccgacaagga gcccgaccgc tcccgtgggc agatcgtcgc ctcggccgtc aaggacgcct
    13801 tcgtggcggg gaaggccaag cgcgcggcca agcgcgccgc caaactggct taaccccttg
    13861 cgggcaggga tagcctcaca gacgtatctc tcgatagtcc ggacacctct ttctggtgtt
    13921 acgtcgtgag gagcgtctct cccatgctca agccaagtgc ccatccgcac ccgatgatca
    13981 ggttgtccga gcgaggcctc tgatggcccg catccccgac tacctcgact tcgacctcga
    14041 actcaccgac gccggtgaag gcggctacac cgcccgggtc ctcgcctccc ccaaggggga
    14101 ggccagtgcc gatttccgta tgccgttcct cggcgtcgat ctcgagaacg tcatcctcaa
    14161 gctcggccgc acccgttcgg gcgtccgcgc gctgggctcc ccgcagcatg acctcgcccg
    14221 caccttcggc ggtcagctct acgacaccgt gttctccgga gaggtcggca cctgcttccg
    14281 gcgcagcctc gatgaggcgg acgagcaggg caagggcctg cgtatcaaac tgcggctcac
    14341 cgacgtcccc tcgctcgccg acgtcccctg ggaatacctg tatgccgcgg ggctgggtcg
    14401 cttcctcgtt ctttccgagt ccacgccgat catccgttac ctcgacctgc cgcgtgaggt
    14461 tctgccgctc accgtcacgc ctccactgcg gattctcttg gccgtgtgca gcccaaccaa
    14521 cctcacccaa ctcgacaccc aggcggaagt cgcgcgggtc gccgaatcgc tccgcgacct
    14581 cgtcgagcgt gggctggtca tcgtggaccc gctgccgaac gcgacgctca cggcgctgcg
    14641 tcaggcgctg cgggcgaccg actatcacgt cctgcacttc atcggtcatg gcggcttcga
    14701 cccggtcgcc ggcgacggcg tgctcgcctt cgaggacgcc gaccatctgg gccacctcgt
    14761 gtccgggcag gacctgggca cgctgctgca cgatatgcgc accctgcgct tggccgtcct
    14821 caacgcctgc gaaggcgctc ggcagtcgcc caccgacccc ttctcgggtg tcgcgcagtg
    14881 tctggtccgt cagggcttgc cggcggtcgt cgctatgcag ttcgagatca ccgataccgc
    14941 agccaccagc ctgtcgtcgg agttctatgc cgctctggcc gacgggctgc ccatcgacgc
    15001 tgctcttgca catgcccgga tgagcgtgtt cgctgcggac aacgacgtcg agtggggcac
    15061 gccggtcctc tatctgcgcg cccgggacgg gcgaatcttc gacgtggcca ctcaagatgc
    15121 ggccccgccg acacccgcgc cgatgcctac gccgacacca gtggcgacac ccgcgccgac
    15181 acccgcgccc gctccaccat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 cctacgccga caccagtggc gacacccgcg ccgacacccg cgcccgctcc accatcaccc
    15361 atcccgatcc cttacccgat ctcgaccact ccaccgactc cggcgccgat gcccgtaccg
    15421 acatccgcaa cacccccgcc gatgcccgtg ccgacgccac ctaccccgca aacgggggcg
    15481 ggcggcatac ctgctgacgc gccgggagca ccgaagcagc gccatcccgc ggtggcaccc
    15541 gccgtcgtcg cagccgtcat cgtcaccttg ggcctggtcg cggcgttctt gctgcggccc
    15601 gatggcggcg cggcgacgaa tggaccgggg ccggcgccca aggagttcaa cgggttcgtc
    15661 gcgatgcgcg gaacgccggt catcgacggc caggccaacg aatgggccgg agccgtgccc
    15721 tacgtctcgg acgtcctcgt cgcgggcaag gcgacgaaga tcaaggcgac gtggagcctg
    15781 atgtgggacc agcaggcctt ctacgtggtc gccgatgtcg ccgaccccaa cctcatgcca
    15841 gccgaccaga cggctcccga ccagcagtgg cgcggagatt cggtgagctt cgagctcggg
    15901 gctgatccgc ggggcttggc cgtcgaggct gggctgcggg ccacggacgc gcactacatc
    15961 gtcggcctga ccccgacgac cccggcgcgg accaagatcg tcatcaaccg tgccaacgtc
    16021 accaagaaca ccatcgacgc cggccgattc catggcgaga gcgacgccgt cgttgcgctc
    16081 attcccggcg gttaccgggt cgaggcgcgc ctgccctggg ccgtgacggg tgtggcgccc
    16141 gttggggtgc agaccggtca ggtgttcggg atcaacctca acgccagcga cggcgggtct
    16201 tcacccaagg agttcgccgc gatgtactcc agcaaccggg gccgcaccag cgccaaccag
    16261 gtccgacccg ctgcctggca ggtcgtgcaa ctgcagggct gacctgggga ggccccgaac
    16321 cccgctggag ctaggcgtcc actgtttgcg cgcggcggct cggtgaggga agatctaccc
    16381 acgggtaacc ccacccggcg acccgactcg ggaggacgac gatggccagg tacgacatca
    16441 cgaccgcaac cttgggcacc ctgctggccg accccgacgt cgtggccatc atggagaagc
    16501 acgcccccgg catcacgacc aaccccatga tcggcatggc ggccggcatg cccgccgccc
    16561 aggccgtcgc catggccggc gggatgatcg gcggagacgc cgtgtcggca atcaccgacg
    16621 aggtcacttc tcttaactga ggtgcatgta gtacagtctt cacagacacc aacacagacc
    16681 cacgagcggc tgccggaccc cctgctggcg gccgctcgtg tttgtgtttc cggggaccat
    16741 tcgcccacat cggcagagtt cggtcgcccg ctcccgcgct aggcaccccc gtttgggccc
    16801 gttccccgtt gtgacgccac ccgccgcggt aggccgccgc taagccacgg tatgccgcgg
    16861 gcgcggcata ccggatagcg tgcggtgatg gccgcgaagg caacgacccc cgcgatcgag
    16921 ctcgctgtcg gtgagcggac gatccggctc agcaaccccg accgggtgta tttcccggag
    16981 cacgggtgga ccaaactcga cctggcgaac tactacctcg cggtcggcga cggcatcgtc
    17041 cgggcgctgc gggagcggcc gtgcatgctg caccggttcc ccgaggggct ggccggcgag
    17101 aaggtccacc agaagcggat cccgcacggg gccccgccgt ggttgcagac cgtgcggctg
    17161 cacttccccc gctggaaccg caccgccgac gaactgtgcg tcaccgagct cgggtcggtc
    17221 atctgggcgg tgcagatgtc gaccgtggag ttccatccgt ggaacgtccg gcgcgccgat
    17281 gtcgatcgcc ccgacgagtg gcggatcgac ctcgacccca tgcccgaggc cgggttcgac
    17341 cgggtgcgcg tcgtcgccgg ggtcgtccgc gaggtgcttg acgagttggg gatcgtcggt
    17401 ttcccgaaga cttcgggcgg caacgggctg cacgtctacg tccggatcgt gcccgagtgg
    17461 gagttcggcg acgtccgccg ggcggccctc gccttcgccc gcgaggtgga gcggcggctg
    17521 cctggagacg cgacgacgac ctggtggcgc aaggaccgcg acccccggca tgtcttcatc
    17581 gactacaacc agaacgcgcg cgaccacacc atcgcggcgg c
//
LOCUS       DAORPW010000041        17052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_635_length_17052_cov_851.680179, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000041 DAORPW010000000
VERSION     DAORPW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 17052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17052
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_635_length_17052_cov_851.680179"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /gene="eno"
                     /locus_tag="PLB86_05295"
     CDS             <1..365
                     /gene="eno"
                     /locus_tag="PLB86_05295"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851223.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRC64583.1"
                     /translation="GDDLFVTNPERLQRGIDTQTANALLVKVNQIGSLTETLDAVDLA
                     HRNGYRCMMSHRSGETEDTTIADLAVATNCGQIKTGAPARSERVAKYNQLLRIEEELD
                     DAAVYAGAGAFPRFAAKG"
     gene            408..959
                     /locus_tag="PLB86_05300"
     CDS             408..959
                     /locus_tag="PLB86_05300"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRC64584.1"
                     /translation="MTTPARRTRVRPGTTATTGRRPGASRPAPAPRTREATPDDDRRG
                     PAWLRWVILGAIAVVLLVTLVPTARSLARQRAEIDALATQVAQQQSQVASLQAEQERW
                     TDPAYVEQQARDRLKFVKVGDRSYTVIDPLEAARPPVSGASVAAPQANGDAPWYGQIW
                     QSVQLADRPTAGMLPVTAPPTAP"
     gene            956..1486
                     /locus_tag="PLB86_05305"
     CDS             956..1486
                     /locus_tag="PLB86_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HRC64585.1"
                     /translation="MTPPTEPTGPTEPEDLAAIAGQLGREPRGVLGIAHRCPCGRPDV
                     VRTAPRLPDGTPFPTTYYATCPRLTAAISTLETQGLMRDMQARLADDEDLAAAYARAH
                     TAYLADRATLASGGDVPEIAGVSAGGMPDRVKCLHVLVAHSLAAGPGINPFGDEALAL
                     LAPWWDAGPCVGAAEE"
     gene            1539..2540
                     /locus_tag="PLB86_05310"
     CDS             1539..2540
                     /locus_tag="PLB86_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRC64586.1"
                     /translation="MVPATAPRATSAAGEVRVGAIDCGTNSLRLLIADIDPQTGKMLE
                     LVRRMEIVRLGCGVDKTGVLAPEALARTLAMTRDYATQCREYAVHRIRFVATSASRDA
                     RNADEFVAGVRAAFGDLDVAPEVVSGQEEARLSFLGATGDLIAAQMEGPYLVVDIGGG
                     STEFVRGTHTVEAARSVDIGCVRLTERHLRSDPPTPDEIAAAIVDIDRAIDMAIETVS
                     LREVRTLVGLAGSVTTVAAQHLDLRAYDPGAIHLSVATPPQIQAACEQLMGMTRAERA
                     AKGFMHPGRVDVIGAGALVWSRVIARVMRQTGLGQALPVATSEHDILDGIALSLVGR"
     gene            2551..3399
                     /locus_tag="PLB86_05315"
     CDS             2551..3399
                     /locus_tag="PLB86_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRC64587.1"
                     /translation="MPQALPHPVTGDLSGSPAPPGSGWPGDPATRHTPRARDAEQVVS
                     LARSASDLATLDARISVCRACPRLVQWREQVAGDKRAAFADQPYWGRPAPGFGDEAAQ
                     VLVVGLAPAAHGANRTGRVFTGDRSGDWLFAALHRAGYASQPTSVHAGDGLTLRGVRI
                     IAAVRCAPPGNQPTVSERATCAPWLDRDLALAAPSLRSILALGGLAWDASLGAARRLG
                     WQVPAPKPTFGHGAMAQLREPGGRVVRLVGSYHVSQQNTFTGRLTESMLDSALALLAW
                     PDTVAE"
     gene            3396..3929
                     /locus_tag="PLB86_05320"
     CDS             3396..3929
                     /locus_tag="PLB86_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRC64588.1"
                     /translation="MSTTPTPVLAVTVLGDDRPGIVADVTAAVAGLGGNLEDSTMTLL
                     RGHFAMVVLVRTPASVADVEAALAGIAAGGSLTVDVRELRESGMPPAPGRAWTLRVHG
                     ADRPGIVASITEVLAAHGGNIVDLGTRLGSDLYVLIAEVELPEAASGEAVAADLRAAA
                     AAVGVTVAFEPVDDEVL"
     gene            3926..4462
                     /locus_tag="PLB86_05325"
     CDS             3926..4462
                     /locus_tag="PLB86_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRC64589.1"
                     /translation="MSSFVMPSLPEGRVLDVVRAPAPVLATPGADVDPAAPSVIQLAA
                     DLLATQAVSPGCVGLAAQQVGVAWRMFSVDVTGHPKTRTCHGEFVLCNAVVESASRKE
                     KGREGCMSVPDFTGDVARARRLVVRGQLPVTGEWVTLETDAFEAVCLQHEIDHTNGLV
                     FLDRVAGPQAVHPRRTYL"
     assembly_gap    4464..4563
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4822..4895
                     /locus_tag="PLB86_05330"
     tRNA            4822..4895
                     /locus_tag="PLB86_05330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4856..4858,aa:Leu,seq:taa)
     gene            5005..5925
                     /locus_tag="PLB86_05335"
     CDS             5005..5925
                     /locus_tag="PLB86_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRC64590.1"
                     /translation="MAGNNPVFNNIQKQLQGGEYAGIGEPRGAQQRYPQQGYPQGNPQ
                     QGYPQGNPQGQQPGYYPPTPMSPEQLDAMYQRPSAGPLDTGRVTFDDVIMKTLTLFAI
                     VLSSAAVTWVMTSAQPALASPIWMVGMFGGLVVGLIIAFKKTISVPLILTYSLLEGAF
                     VGALSRAMEAWYPGIVMTAVVATLATFAGMFIGYRTGVIKVTSRSRRIFGMAIMGYML
                     FSLVNVLALVFGWTSGWGFGGTGLLGIGISILGVGLASYSLAVDFDSVEEGIRMGAPE
                     KFSWLFAHGIIVSLVWLYIEILRLLARLRD"
     gene            5972..6262
                     /locus_tag="PLB86_05340"
     CDS             5972..6262
                     /locus_tag="PLB86_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64591.1"
                     /translation="MITVPDPVRIELERLDGRWRQLPLDQALRAVPAVRALVQALADE
                     VAAARGMPSAPVPDHGPAVALDQLRVMAFDAVQAGLDHLLAERLADLRRTLA"
     gene            complement(6361..7584)
                     /locus_tag="PLB86_05345"
     CDS             complement(6361..7584)
                     /locus_tag="PLB86_05345"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRC64592.1"
                     /translation="MTDAVIVSTARSPIGRAFKGSLKDMRPDDLSVQMIAAALAKIPA
                     LNPADIEDLYWGCAEPSGKQGANMARVIAVLAGFDHLPASTVNRFCASSVQTTRMAFH
                     AIKAGEGDVFLSGGVECVSQYGKFAGAGGSDANDQNPLFQPAITRSEVMAVTNEVWTD
                     PRELGIIPDVYLSMGQTAENVATSRGISRARQDEWGVASQNRAEAAINSGFFTKDITP
                     VTLPDGTQWSTDDGPRAGVTLEAVSGLKTVFRENGTVTAGNCCPLNDGAAAVVVMSDR
                     KAKELGLTPLARVVATAATGLSPEIMGLGPVEATRKALRLAGMSISDIDLYEINEAFA
                     VQVLGSADELGMDFDKLNVHGGAIALGHPFGSTGARITSTLLNGLAERDGTFGLETMC
                     VGGGQGMAIIYERLS"
     gene            7720..9099
                     /locus_tag="PLB86_05350"
     CDS             7720..9099
                     /locus_tag="PLB86_05350"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HRC64593.1"
                     /translation="MRYAEHISDLVGNTPLVRLTKVTEGIAATVLAKVEYLNPGGSVK
                     DRIALRMIEAAERSGELRPGGTIVEPTSGNTGVGLALVAQRKGYRCVFVCPDKVSEDK
                     RNVMKAYGAEVVVCPTAVAPEHPDSYYNVSDRLVREIEGAWKPNQYANPEGPASHYAT
                     TGPEIWGDTDGRVTHFVAGVGTGGTISGTGHYLKDQAAAVGRPGQVHVIGADPEGSVY
                     SGGTGRPYLVEGVGEDFWPTAYDPAVVDEVVAVTDADSFAMTRRLAREEGLLVGGSCG
                     MAVVAALQIARDLPADAVVVVLLPDGGRGYLSKVFADQWMNSYGFLREAGERTVGEIL
                     HAKSGALPALVHTHPSETIREAIDILREYGVSQMPVVKAEPPVMSGEVAGSVSERALL
                     EALFTGAAHLSDSVERHMEPGLPLVGAGEPVSVARTELEHADAVLVVEEGKPVGVLTR
                     ADLLGFLVG"
     gene            complement(9121..9528)
                     /locus_tag="PLB86_05355"
     CDS             complement(9121..9528)
                     /locus_tag="PLB86_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64594.1"
                     /translation="MSPITVVASGELTAIASATTAAAASAPAYLPALLLTLVVEVPAL
                     LLLARLFARVSVPLALAGAVGVNLLTHPILYAASAGFSSWPQLLAAEALVALVEGLAL
                     AWVWRVPLRESWPLVGAFAVNALSTLAGLALYR"
     gene            9551..10369
                     /locus_tag="PLB86_05360"
     CDS             9551..10369
                     /locus_tag="PLB86_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64595.1"
                     /translation="MLELRYVLGRFGDLLTGTVGLLLVVLVSGIAVARLGGAYLGGWS
                     RPVEILLGYAVLGAGAWIGLRGVARVVVLAVLVGAAAVSLSHPAYHVVVLAHLHNLVP
                     LVFLWDWARRLPVRARGWFRGVQVGWVLVAPAVVLAGVFDRWVDADPGAVRALVGEGA
                     QVVSSVAWPAAPEAAMRWLVAFAFLQTMHYVVWVWFLPRAAPEATAAFEARWPVASSR
                     RVWGVGVALAVALGALLLTDYGQGRTVYSGLATYHAYLEFPVILVLLTRWRRSP"
     assembly_gap    10709..10808
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10855..12060)
                     /locus_tag="PLB86_05365"
     CDS             complement(10855..12060)
                     /locus_tag="PLB86_05365"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRC64596.1"
                     /translation="MSREIRRIALLTAGGYAPCLSSAVGGLIERYSQVLPDAEIIGYL
                     HGYHGVLAGNYLVVDQETRDKAHLLHDFGGSPIGNSRVKLTNVKDLVKRGLVKEGENP
                     LHVAAERLKADGVDVLHTIGGDDTNTTAADLAAYLHEAGYELTVVGLPKTIDNDVVPI
                     RQSLGAYTAAEEASLFAQNILAENGSNPRMLIIHEVMGRGCGWLTGKAASNYQAWHAE
                     QEWVPSFGLSAERWAIHGVYVPEIHIDLDAEAARLTKVMDEVGCVNLFLSEGAGVPDI
                     VAAMEAAGQEVPRDPFGHVKLDMINPGQYFAKQFAEKLGAEKTMVQKSGYFSRSARAN
                     AQDLELIAAMCDKAVEAAMAGIPGVVGHDEERGDELRAIEFPRIAGHKAFDTSVDWFQ
                     DVLRTTGQA"
     gene            complement(12235..12384)
                     /locus_tag="PLB86_05370"
     CDS             complement(12235..12384)
                     /locus_tag="PLB86_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64597.1"
                     /translation="MGPGPYAAPPPGVPRKSNAGKTVAIVILTVIGGSALLMIVLAGV
                     CLSQL"
     gene            complement(12657..12977)
                     /locus_tag="PLB86_05375"
     CDS             complement(12657..12977)
                     /locus_tag="PLB86_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64598.1"
                     /translation="MTNPPVTPGIPGVPVAALPEATAPFDPLRLCIFATVALLGWALG
                     PFALLFFAGLGFTGYWKARQAGLTRSRCLLRDTRLVLTYLAVLAAAACVGIWFLLTRL
                     GRLT"
     gene            complement(12974..14545)
                     /locus_tag="PLB86_05380"
     CDS             complement(12974..14545)
                     /locus_tag="PLB86_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC64599.1"
                     /translation="MPLRDFRIHRYVNAFCPHCHAERPDQPLADVRRLSGWLAARDGK
                     VWLERGCPEHGFVRTLYDESAEILTYLEQWTATTKEHVSDVMGNFAPVPAAYLDGMPH
                     MQTQHTCILLADITDTCNLRCPTCFAESAPARATTAPLAEVLASVDDRLAKERGRLDV
                     VMLSGGEPTLHPDFLRLLDELVARPIIRILVNTNGIRLAQDDALLAALVRHRERVEVY
                     LQFDGLTPTGSIHHRGADLLRFKKAALERLTGAGVFTTLTLTAALGVNDDQIGPVIQL
                     ALQTPFVGGVTIQPVFGSGRSAGIDPVDRLTHTGVLARLGEQTDGQITWRDLTALPCS
                     HPHCASVGYFLRDDSGQWRSLVSLIGPDRLKDYLDLAPDEFANRFADQDIPARLRASV
                     KDSLLGLLSEQSSVSHPDIGRIWQDICTTCDIGIGTLTTLAASRLPGQAGRLRTMLAE
                     RVLRITVKPFMDINTMIEERLTQCCVHVGTVNPGDGSHQCAPFCAVQAWAPLSRTRLS
                     TAAGSLTRPPAEVPS"
     gene            complement(14572..15384)
                     /locus_tag="PLB86_05385"
     CDS             complement(14572..15384)
                     /locus_tag="PLB86_05385"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRC64600.1"
                     /translation="MFPTLGDLFGIVLAVPTHDTFVGLGVMVAVAVFIVETRRRGVAD
                     YRLLYVVTGALVGGAIFMRLGPVLQHLDLGANPSLAQQWVYGNRSVLGGLFGAWLGVH
                     VSKRLAGYSPRTGDLFAPAVAIGMAIGRFGCLFTELPGSPNPLGFGPVLDDATAARLS
                     GVAGTPLHPSYAYEIAFHAIAFVVLWRVLRHRMQAPGETLVVYLAAYGVFRFCVEFTR
                     GEEAVWAGLDRSQLFLLATVPILLARVVWQWRRGAYVLAPRHTPAQLAGSLP"
     gene            complement(15394..15885)
                     /locus_tag="PLB86_05390"
     CDS             complement(15394..15885)
                     /locus_tag="PLB86_05390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC64601.1"
                     /translation="MQIRDAKPDDLPAIAPIFREIVADGETYAYPEDLTDAQIAAAWM
                     EPPPARCIVAYAADGTILGTAKAGPNRPGRGDHIATASFMVSPTAQGQGVGRALAEEV
                     LRWATDQGYAAMQFNAVVETNMGAVALWQQLGFRILTTVPGTFRSRHHGPVGLHVMFR
                     ELG"
     gene            15917..>17052
                     /locus_tag="PLB86_05395"
     CDS             15917..>17052
                     /locus_tag="PLB86_05395"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HRC64602.1"
                     /translation="MADSTSGADDLVLEATEQRVLGSLLEKQVTVPSTYPMSLNAIRS
                     ACNQATSREPVTDFDEATVQESLRGLRLRELVRVVHGDRVLKYHQLLGQRLELADDER
                     ALITVLLLRGPQSPGELKTRTDRLHAFADRTEVEECLARLASREVPLVVELPKAPGHH
                     DARWVHLLGPVELPAAVAAAVAAPVVDREVVLAGGAAQRDVNVTAAYDAVATAYALEF
                     GTELEGKPFDRWLLARVAQLAGIDPVADVGCGPGHVTRFLAEAGASVSGFDVSPGMVE
                     EARLGSPDLGFEVADFRGLMRPRLAPAWGAIVAWYAVIHLAESELASAVAGLARVLRP
                     GGWLALATHVGDAVHHVEEFLGEPVQLDFVLHDATAVREAVAAAG"
BASE COUNT         2390 a         5819 c         6047 g         2596 t
ORIGIN      
        1 tcggcgacga cctgttcgtc accaaccccg agcggctgca gcgcggcatc gacacccaga
       61 cggccaacgc cctgctcgtc aaggtcaacc agatcggctc gctcaccgag acgctcgacg
      121 cggtcgacct ggcccaccgc aacggctacc gctgcatgat gagccaccgc tccggcgaga
      181 ccgaggacac gacgatcgcc gacctcgccg tcgcgaccaa ctgtggtcag atcaagaccg
      241 gcgccccggc ccggtccgag cgcgtggcga agtacaacca gctcctgcgc atcgaggagg
      301 agttggacga cgccgcggtg tatgccggtg ccggcgcctt cccgcgtttc gctgccaagg
      361 gctgagccgt tgaggggtca gactgggggg atggggacct ccggggcatg accacaccag
      421 ctcgacgcac gcgcgtgcgc cccgggacga cggccaccac cggccgtcgc cccggggcca
      481 gccgccctgc ccccgcacct cggacccgtg aggcgacccc cgacgacgat cgtcgcggcc
      541 cggcctggtt gcgctgggtc atcctcggcg ccatcgccgt cgtcctgctc gtcaccctcg
      601 tgccgaccgc ccgatccctg gcccgacagc gggccgagat cgacgccctg gcgacccaag
      661 tcgcccagca acagagccag gtggcctcgc tgcaggccga gcaggagcgg tggaccgacc
      721 ccgcctacgt cgagcagcag gcccgtgacc ggctgaagtt cgtcaaggtc ggtgaccgct
      781 cctacaccgt catcgacccg ctggaggccg cccggcctcc cgtctccggc gcttcggtcg
      841 ccgcgcccca ggccaacggc gacgccccct ggtatggcca gatctggcag tccgtgcaac
      901 tggccgaccg tccgaccgcc ggcatgcttc cggtcaccgc gccgccgacc gccccgtgac
      961 gccgccgact gagccgactg ggccgaccga gcctgaagac ctcgccgcga tcgccgggca
     1021 gttgggccgg gagccacgcg gagtgctcgg aattgctcac cgctgcccgt gcggtcgtcc
     1081 cgacgtcgtc cgcaccgccc cgcggctgcc cgacggcacc ccgttcccga cgacctacta
     1141 cgccacgtgt ccccggctga ccgccgcgat cagcaccctg gagacccaag gtctgatgcg
     1201 cgacatgcag gcccggttgg ccgacgacga ggacctggcg gccgcctacg cgcgggcgca
     1261 cacggcatac ctcgccgatc gggccaccct cgcgtccggc ggcgacgtcc ccgagatcgc
     1321 gggcgtctcc gcgggtggga tgcccgaccg ggtcaagtgc ctgcacgtcc tcgtcgcaca
     1381 ctcgctggct gccggccccg ggatcaaccc gttcggcgac gaggccctag cgttgctggc
     1441 cccctggtgg gacgccgggc cgtgcgtggg agccgccgag gagtgacccg gcggagtccg
     1501 ggggtgccgt gtcgggggag cgatgggata atcgccgcat ggtccccgcc accgcgcctc
     1561 gcgccacttc cgccgccggg gaggtccgag tcggggccat cgactgcggg accaattcgc
     1621 tgcgactgct tatcgccgac atcgatccgc agaccgggaa gatgcttgag ctggtgcggc
     1681 ggatggagat cgtccggctg ggctgcgggg tcgacaagac gggggtgctc gcccctgaag
     1741 cgctggcgcg cacgctggcg atgacccgcg actacgcgac ccaatgccgt gagtatgccg
     1801 tccaccgcat ccggttcgtc gccacctcgg cctcccgcga cgcgcgcaac gccgacgagt
     1861 tcgtcgccgg ggtccgtgca gcgttcgggg acctcgacgt cgcacccgag gtcgtcagcg
     1921 gccaagagga ggctcggctg tccttcctcg gggccaccgg tgacctcatc gccgcgcaga
     1981 tggaggggcc ctatctcgtc gtcgacatcg gcggcggctc cacggagttc gtccggggca
     2041 cccacacggt ggaggccgct cgctcggtcg acatcggttg cgtgcggctc accgagcgcc
     2101 acctgcgcag cgacccgccg acccccgacg agatcgccgc ggcgatcgtc gacatcgacc
     2161 gggccatcga catggccatc gagacggtca gcttgcgtga ggtgcgcacc ctcgtcgggt
     2221 tggccggcag cgtgacgacc gtggcggccc agcacctcga cctgcgggcc tacgacccgg
     2281 gagcgatcca cctgtcggtc gccacgcccc cgcagatcca ggcggcgtgc gagcaactca
     2341 tggggatgac ccgggcagag cgcgcggcca agggcttcat gcaccccggc cgggtcgatg
     2401 tcatcggtgc gggggccctg gtctggtcgc gggtcatcgc ccgcgtcatg cgccagactg
     2461 gtctcggcca ggccctgccc gtcgctacct ccgagcacga catcctcgac ggcatcgccc
     2521 tgtcgctcgt cggccgctga gtcccgcgcc gtgcctcagg cgttgccgca tccggtcacg
     2581 ggggacctgt cgggctcgcc tgcaccccct ggctctgggt ggcctgggga cccggcgacg
     2641 cggcataccc ctcgggctcg tgacgccgag caggtggtga gcctcgcgcg gtcggcctcc
     2701 gacctcgcca ccctcgacgc gcggatctcg gtatgccgtg cctgcccgcg cctggtccag
     2761 tggcgtgagc aggtggcggg ggacaaacgg gcggcgttcg ccgaccagcc ctactggggg
     2821 cgaccggcgc cgggcttcgg cgacgaggcc gcgcaggtgc tcgtcgtcgg gctggcccct
     2881 gccgcgcacg gtgccaaccg caccggccgg gtcttcaccg gcgaccgcag cggcgactgg
     2941 ctcttcgccg cgctgcaccg ggccggttat gcgagtcagc cgacgagtgt gcatgcgggc
     3001 gacgggctga cgctgagagg cgtgcgcatc attgcggccg tccggtgcgc gccgccggga
     3061 aaccaaccga cggtgtccga gcgggcgacg tgcgcgccgt ggttggaccg cgacctcgcg
     3121 ctcgctgcgc cctcgttgcg ctcgatcctg gcgctgggcg gtctggcctg ggacgcgtcg
     3181 ctgggcgcgg cccgccggct cggctggcag gtcccggccc ccaagccgac gttcgggcac
     3241 ggcgcgatgg cgcagttgcg cgagccaggc ggacgcgtcg tgcggctggt cggcagttac
     3301 cacgtgagcc agcagaacac cttcaccggg cggctcaccg agtcgatgtt ggactcagcg
     3361 ctggcgctgc tcgcgtggcc cgataccgta gccgagtgag cacaaccccc actcccgtcc
     3421 tggcggtgac cgtcctcgga gacgaccggc ccggcatcgt cgccgacgtc accgccgccg
     3481 tcgccgggtt gggcggcaac ctcgaagact ccacgatgac gttgctgcgc gggcacttcg
     3541 cgatggtcgt gctcgtgcgc acccccgcgt cggttgctga cgtcgaggcc gcgctggccg
     3601 ggatcgcagc cggaggctcg ctcaccgtcg acgtccgcga gttgcgggag tcgggtatgc
     3661 cgcccgctcc cggccgcgcc tggaccctgc gggtccacgg ggctgaccgg cccgggatcg
     3721 tcgcctcgat cactgaggtg ctggccgctc acggcggcaa tatcgtcgac ctgggcaccc
     3781 gcttgggatc cgacctctac gtgctcatcg ccgaggtcga gttgccggaa gccgcgtccg
     3841 gcgaggccgt cgcggccgac ctgcgcgcag ccgccgctgc ggtcggcgtc accgtggcct
     3901 tcgagccggt ggacgacgag gtgctgtgag ttcgttcgtc atgccctcgc tccctgaggg
     3961 tcgagtcctc gatgtcgtgc gcgcccccgc gccggtgttg gccacccctg gggctgatgt
     4021 cgatccggcc gcgccgtcgg tcatccaact ggccgccgac ctgttggcca cccaggcggt
     4081 gtcgccgggg tgtgtggggc tggccgctca acaggtgggc gtggcctggc ggatgttctc
     4141 ggtcgacgtc accgggcacc ccaagacccg gacgtgtcac ggcgagttcg tcctgtgcaa
     4201 cgcggtcgtc gagtcggcca gtcgcaagga gaagggccgc gaggggtgca tgtcggtgcc
     4261 cgacttcacc ggcgacgtgg cccgggcgcg gcggttggtg gtgcgcgggc agttgccggt
     4321 cacgggggag tgggtgaccc tggagaccga tgccttcgag gccgtctgcc tgcagcacga
     4381 gatcgatcac accaatggcc tggtcttcct cgaccgagtc gccggccccc aagccgtcca
     4441 ccccagacgc acctacctct aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnctcgacc gagtcgccgg cccccaagcc gtccacccca gacgcaccta cctctaactc
     4621 gcgccaggcc ctcctagacc ccaccctcct agcccccaca caccggcccc cggtttcccc
     4681 gaattcatca cactcgccgg cgttctgggc aatcccaggc cttgagcgac ccgaatccct
     4741 cacagtggcc agtgttctgg gccggaggag caacacgggc cttgatgctc cctggcccca
     4801 gcccctccta gagttgacct tgccctcgta gcccaatcgg cagaggcggg cgacttaaaa
     4861 tcgcctcagt gcgagttcga gtctcgtcgg gggcacgcat tcgtgaccgt ccggtgaccg
     4921 ggccaaactt cgggaacacc ggcgacgccc catacgctgt accgaccaga cggacccgca
     4981 gggcccgaca ctcatggagg acacatggcc ggcaacaacc cggtgttcaa caacattcag
     5041 aagcaactgc agggcgggga gtatgccggt atcggcgagc cccgtggcgc gcagcagcgc
     5101 tacccgcagc agggctaccc ccagggcaac ccccagcagg gctaccccca gggcaacccg
     5161 caggggcagc agccgggcta ctacccgccg acgccgatga gccccgagca gctggacgcg
     5221 atgtaccagc gtccgagcgc cgggcctctc gacaccggcc gggtgacctt cgacgacgtc
     5281 atcatgaaga cgctcacgct cttcgcgatc gtcctcagct ccgcggccgt gacctgggtg
     5341 atgacctcgg cccagccggc cctggcctcg ccgatctgga tggtcgggat gttcggtggc
     5401 ctggtcgttg gcctgatcat cgccttcaag aagacgatct cggtcccgct gatcctcacc
     5461 tactcgctgc tcgaaggcgc gttcgtcggt gccctcagcc gagccatgga ggcctggtac
     5521 ccgggcatcg tcatgaccgc cgtcgtcgcc acccttgcga ccttcgcggg catgttcatc
     5581 ggctaccgga ctggcgtcat caaggtgacc tcgcgctccc ggcggatctt cggcatggcg
     5641 atcatgggct acatgctgtt cagcctggtc aacgtcctcg cgctggtctt cggctggacg
     5701 agcggctggg gcttcggcgg caccggtctg ctgggcatcg gcatctcgat cctcggcgtc
     5761 ggcttggcct cctactcgct ggccgtcgac ttcgactcgg ttgaggaggg catccggatg
     5821 ggcgcccccg agaagttctc gtggctgttc gcccacggaa tcatcgtcag cctggtgtgg
     5881 ctctacatcg agatcctgcg cctgctggcg cgcctgcgcg actgaccgcg cgagcgatca
     5941 gcccaacgac cgacagcacc ggtgtgacct cgtgatcacg gtgcccgacc cggtccgcat
     6001 cgagctcgag cgcctggacg ggcggtggcg acagctgccg ctcgaccagg cgcttcgtgc
     6061 tgtcccggcc gtccgcgcgc tcgtccaggc cctcgcggac gaggtcgccg cagccagggg
     6121 tatgccgtcc gcccccgttc ccgatcacgg cccggccgtc gccctggacc agttgcgagt
     6181 catggccttc gacgccgtcc aggccggctt ggaccacctg ctcgccgaac gactggccga
     6241 cctgcgccgc accctcgcct gacgcccgca gccgctcact cgaagcagcc tccgcacgaa
     6301 aaggtatgcc gcccgctcag gtgagcgggc ggcatacctc atgcctgggg gagccagagc
     6361 tcagctgagg cgctcgtaga tgatcgccat gccctgcccg ccgccgacgc acatcgtctc
     6421 cagcccgaag gtgccgtcgc gctcggcgag cccgttgagc agcgtcgagg tgatccgggc
     6481 gccggtcgag ccgaacgggt ggccgagcgc gatcgccccg ccgtggacgt tgagcttgtc
     6541 gaagtccatc cccaactcgt cggccgagcc gagcacctgg acggcgaagg cctcgttgat
     6601 ctcatagagg tcgatgtcgc tgatgctcat gccggccaac cgcagcgcct tgcgggttgc
     6661 ctcgaccggg ccgaggccca tgatctcggg cgagaggccg gtggcggcgg tcgcgacgac
     6721 ccgggccagc ggcgtgaggc cgagctcctt ggccttgcgg tcgctcatga cgaccaccgc
     6781 ggcggcgccg tcgttgagcg ggcagcagtt gccggcggtc acggtgccgt tctcgcggaa
     6841 gaccgtcttg agcccagaca cagcctcgag ggtcacgccc gcgcggggcc cgtcgtcggt
     6901 cgaccattgc gtcccgtcgg gcagcgtcac cggggtgatg tccttggtga agaaaccact
     6961 gttaatcgca gcctcggcgc ggttctgcga cgcgacgccc cactcgtcct gacgcgcccg
     7021 gctgatgccg cggctggtcg cgacgttctc ggcggtctga cccatcgaca ggtagacgtc
     7081 ggggatgatg cccagctcgc gggggtcggt ccacacctcg ttggtgaccg ccatgacctc
     7141 gctgcgggtg atcgccggct ggaagagcgg gttctggtcg ttggcgtccg agccaccggc
     7201 gccggcgaac ttgccgtact gcgacacaca ctcgacgcca ccggagagga acacgtcgcc
     7261 ctcgcccgcc ttgatcgcgt ggaaggccat ccgggtcgtc tgcaccgacg aggcgcagaa
     7321 ccggttgacg gtcgaggccg gcaggtggtc gaagcccgcg agcacggcga tgacgcgggc
     7381 catgttggcg ccctgcttgc ccgacggctc ggcgcacccc cagtagaggt cctcgatgtc
     7441 ggcggggttc agcgcgggga tcttggccag cgcggcggcg atcatctgca ccgacaggtc
     7501 gtcggggcgc atgtccttga gcgagccctt gaatgcgcgc ccgatcgggc tgcgggcggt
     7561 ggagacgatg acggcgtcag tcactggggg ttcctcctcg tcgagggtgc gcgcagccgc
     7621 cggtgagcgg gcgcttagtt cagggttgat cgtatgccgt gcgccccgca cctgcctacc
     7681 ttcgcccgcg gtgaccttca cctctgtgat gatggcggga tgagatacgc cgaacacatc
     7741 agcgacctcg tcggcaacac gcccttggtg cgcctcacga aggtcaccga gggcatcgcc
     7801 gccaccgtcc tggccaaggt cgaatacctc aaccccggcg ggtccgtgaa agaccggatc
     7861 gccctgcgga tgatcgaggc agccgagcgc tccggggagt tgcgccccgg cggcaccatc
     7921 gtcgagccga ccagcggcaa caccggggtg gggctggccc tcgtcgccca acgcaagggc
     7981 taccgatgcg tgttcgtctg cccagacaag gtgagtgagg acaagcgcaa cgtcatgaag
     8041 gcttacggcg ccgaggtcgt cgtctgcccg acggccgtcg cgcctgagca ccccgactcc
     8101 tattacaacg tctccgaccg gctggtccgc gagatcgagg gcgcctggaa gcccaaccaa
     8161 tacgccaacc ccgaaggccc cgcgagccac tacgcgacga ccggcccgga gatctggggc
     8221 gacaccgacg ggcgggtcac ccacttcgtc gccggtgtcg gcacgggcgg cacgatttcc
     8281 ggcaccggcc actacctcaa ggaccaggcc gctgcggtcg ggcgccccgg ccaagtgcac
     8341 gtcatcggtg ccgacccgga gggctcggtc tactccggcg ggaccgggcg gccctacctc
     8401 gtcgaggggg tcggcgagga cttctggccc acggcatacg accccgcggt cgttgacgaa
     8461 gtcgtggcgg tcaccgacgc ggactcgttc gcgatgaccc ggcgactggc gcgcgaggag
     8521 ggcctgctcg tcggagggtc gtgtgggatg gcggtcgtcg ccgccctgca gatcgcccgc
     8581 gacctgccgg ccgacgcggt cgtcgtcgtc ctgctgcccg acggcgggcg cggctatctc
     8641 tccaaggtct tcgccgacca gtggatgaac tcctacggct tcctgcgcga ggccggcgag
     8701 cggaccgtcg gcgagatcct gcacgccaag tcgggcgcgc tgcccgcgct cgtccacacc
     8761 catccgagcg agacgatccg cgaggccatc gacatcctgc gcgaatacgg cgtctcgcag
     8821 atgccggtcg tcaaggccga gccgccggtc atgtccggtg aggtcgccgg gtcggtgagc
     8881 gaacgcgccc tgttggaggc cttgttcacg ggcgccgcgc acctgagcga ctcggtcgag
     8941 cggcacatgg agcccggatt gccgctggtc ggcgcaggcg agccggtcag cgtcgcccgc
     9001 acggagctcg agcacgccga cgcggtgctc gtcgtcgagg aaggcaagcc agtcggagtg
     9061 ctcactcggg ccgacctgct cggcttcctc gtcggctgac tccgctcgtc ggagcgcgcg
     9121 tcagcggtag agcgccaggc ccgcgagggt cgacaaggcg ttgaccgcga atgcgcccac
     9181 cagcggccag gattcgcgaa gcggcacccg ccagacccag gccagcgcga ggccctcaac
     9241 gagcgcgacg agggcctcgg cggccagcag ttgcggccac gaggagaagc cggcggacgc
     9301 ggcatacaga atggggtgcg tgagcaggtt gaccccgacc gcgccggcca gcgcgagcgg
     9361 caccgacact cgcgcaaaca gccgcgcgag cagcagcagt gcgggcacct cgacgaccag
     9421 cgtgagcagc agcgccggca ggtatgccgg tgcgctggct gccgcggcgg tggtggcgct
     9481 cgcgatcgca gtgagttcgc cgctggcgac cacagtgatc gggctcatct gcttcgggat
     9541 ccttcacaac gtgctcgaat tgcgttacgt cctggggcga ttcggtgatc tgctgactgg
     9601 gacggtcggg ctgctgctcg ttgtactcgt cagtgggatc gctgtggccc ggttgggggg
     9661 agcctatctg ggcggttggt cccggcccgt cgagatcctt ctcgggtatg ccgtcctcgg
     9721 cgccggcgcg tggatcggcc ttcgcggagt ggcccgtgtc gtggtgctgg ccgtcctcgt
     9781 cggcgcggct gcggtgtcgc tgagccaccc ggcataccac gtggtggtgt tggctcacct
     9841 gcacaacctc gtgccgttgg tgttcctctg ggactgggcg cgccgcctgc cggtgcgtgc
     9901 ccgcgggtgg ttccgcggtg tccaggtcgg ctgggttctc gtcgcccctg ccgtggtgct
     9961 ggccggggtc tttgaccgct gggtggatgc cgacccaggg gccgtgcgcg ccctcgtcgg
    10021 cgagggggcg caggtcgtgt cgtcggtcgc gtggccggcc gcgccggagg ccgcgatgcg
    10081 ttggctggtc gccttcgcgt tcctgcagac gatgcactac gtcgtctggg tgtggttcct
    10141 gccgcgcgcc gctcccgagg cgacggcggc cttcgaggcg cggtggccgg tggcgagctc
    10201 gcggcgggtg tggggcgtgg gggtcgccct ggcggtggct ctcggcgcgc tcctgctcac
    10261 cgactatggt caagggcgaa cggtctactc ggggttagcg acctatcacg cctatcttga
    10321 gttccccgtg atcctcgtcc tgctcacccg ttggaggcgt tcgccgtgag agtgtccctg
    10381 gtcactgacg tggcaaccgc ccccatggtc gccggcttgg tcgtcttcct cgtcctgggg
    10441 ttcgtcgtcg tggtcggact ggtcatcctg gccatcctgc tggtgcgccg cagccgtcgc
    10501 tagccctgcg ccccaccacc cggggatgaa gccgcgcgtg aagccgcggc aggtggtgtg
    10561 atcacgccac ctgccgcggc ttcatgagcg gccccacgcc cggccgagcg gcatacctgg
    10621 tgcccgcttg ggccccgtcg ctcaggctgt ctctcgtgcc tggctccttg gcacttgctt
    10681 cgctgcgcgc cttcgtcgct caggctgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnntc gtgcctggct ccttggcact tgcttcgctg cgcgccttcg tcgctcaggc
    10861 ttgaccggtg gtgcgcagca cgtcctggaa ccagtcgacg ctcgtgtcga aggccttgtg
    10921 cccggcgatc cgggggaact cgatggcgcg caactcgtcg ccgcgctcct cgtcgtggcc
    10981 gacgacaccc gggatgccgg ccatggcggc ctcgacggcc ttgtcgcaca tcgccgcgat
    11041 gagctcgagg tcctgggcgt tggcccgcgc cgaacggctg aagtagcccg acttctggac
    11101 catcgtcttc tcggcaccga gcttctcggc gaactgcttg gcgaagtact gaccggggtt
    11161 gatcatgtcg agcttgacgt ggccgaacgg atcgcggggg acctcctggc cggcggcctc
    11221 catggccgcg acgatgtcgg ggacgccggc gccctcggac aggaagaggt tgacgcagcc
    11281 gacctcgtcc atgaccttcg tcaggcgggc ggcctcagcg tcgaggtcga tgtggatctc
    11341 cgggacgtag acgccgtgga tggcccaccg ctccgcggac aacccaaagc tcggcaccca
    11401 ctcctgctcg gcgtgccacg cctggtagtt gctcgcggcc ttgccggtca gccaaccgca
    11461 gccccggccc atgacctcgt ggatgatgag catccgcggg ttggagccgt tctccgcgag
    11521 gatgttctgc gcgaagagcg acgcctcctc ggcagccgtg taggcgccga gtgactggcg
    11581 gatcggcacg acgtcgttgt cgatcgtctt gggcaggccg accaccgtca gctcgtaccc
    11641 ggcctcgtgc aggtatgccg cgaggtcggc ggccgtcgtg ttcgtgtcgt cgccgccgat
    11701 ggtgtgcagg acgtcgacgc cgtcggcctt gaggcgctcg gccgcgacgt gcagggggtt
    11761 ctcgccctcc ttgaccagcc cacgcttgac caggtccttg acgttggtca gcttgacccg
    11821 actgttgccg atcgggctgc cgccgaagtc gtgcagcagg tgggccttgt cgcgggtctc
    11881 ctggtcaacg accaggtagt tgccggcgag caccccgtgg tagccgtgca ggtagccgat
    11941 gatctcggcg tccggcaaca cctgcgaata gcgctcgatg aggcctccga ccgcggagga
    12001 cagacacggg gcatagcccc cggcagtgag cagcgcgatg cggcggatct cgcgagacat
    12061 gggcgggcac cttccaggct gtgggcagtc gcctccaacc ctattggggt atgccgcccg
    12121 cccggcgtcg ggtgcggcta acggcactgg cgcgccccgg gtggcccgct gcgcagacgg
    12181 gtttcatcgt cggttcgtcg agtcggtctg ctcgaagcat ctggagcgag gtgctcagag
    12241 ctgggagagg cacacgccgg cgagaacgat catcaacaag gcggacccgc cgataacggt
    12301 gaggatgaca atggcaacgg tcttgcccgc gttgctcttg cgggggacgc cgggcggcgg
    12361 cgcggcatac gggccgggtc ccatcggcgg ggcaccgtac gggccgggtc ccatcggcgg
    12421 ggcaccgtac gggccgggtc ctatcggcgg ggcaccgtac gggccgggtc ctatcggcgg
    12481 ggcgccgtac ggaccaggtg cgatcggcgg gggcggcggg gcaccgtagg ggccgggtgc
    12541 catgggcggc ggcggcgcgg catacggacc aggcgggatg ggcggcggcg ggggcgcctg
    12601 cgccggcggg aactgcgggt cgaagggcga cgggccggaa tcaggcgggg gctgggtcat
    12661 gtgagtctcc cgagacgggt gagaaggaac cagatgccga cacacgcggc ggcggcgagg
    12721 acggcgaggt aggtgaggac caagcgggtg tcgcgcagca ggcagcgact gcgggtgagg
    12781 ccggcctggc gggccttcca gtagccggtg aaccccagcc ccgcgaagaa cagcagcgcg
    12841 aacggcccga gggcccaacc gagcaatgcc accgtggcga agatgcacaa ccgcaaggga
    12901 tcgaagggcg ccgttgcctc gggcagggcg gccacaggca ctccaggtat gccgggtgtc
    12961 acgggtgggt tggtcaactc ggcacctccg cgggtggtcg ggtgagggag ccggctgcgg
    13021 tgctcaggcg agtccgggac agcggggccc aggcttggac cgcgcagaac ggcgcacatt
    13081 ggtgactgcc gtcgcccggg ttgacggtgc cgacgtggac gcagcactgg gtgaggcgtt
    13141 cctcgatcat cgtattgatg tccatgaacg gcttgacggt gatccgcaac acccgctcgg
    13201 cgagcatggt gcgcaggcgg ccggcctggc ccggcaggcg tgacgcggcg agcgtcgtca
    13261 gggtgccgat cccgatgtcg caggtggtgc agatgtcctg ccagatccgc ccgatatcag
    13321 ggtgggagac cgacgactgc tcgctgagca ggccgagcag gctgtccttg accgaggcgc
    13381 gcagccgcgc ggggatgtcc tggtcggcga accggttggc gaactcgtcc ggagcgaggt
    13441 cgagatagtc cttgagccgg tccggaccga tgagcgacac gagcgagcgc cactgacccg
    13501 agtcgtcgcg caggaagtag ccgaccgagg cgcagtgcgg gtgcgaacag ggcaaggcgg
    13561 tgagatcgcg ccaggtgatc tgcccgtcgg tctgctcgcc gagccgggcc aggacgccgg
    13621 tgtgggtgag tcggtcgacg gggtcgatgc ccgcgctgcg tcccgagccg aagaccggct
    13681 ggatggtcac gccgccgacg aagggtgtct gcagggccaa ctggatgacc ggaccgatct
    13741 ggtcatcgtt gactccgagc gccgcggtga gggtcagggt cgtgaacacg cccgcgccgg
    13801 tgaggcgctc gagcgccgcc ttcttgaacc gcaacaggtc ggctccgcga tggtggatcg
    13861 acccggtcgg cgtgagcccg tcgaactgga ggtagacctc gacccgctct cggtgacgca
    13921 ccagggcagc cagcagtgcg tcgtcctggg cgaggcgaat cccgttggta ttcaccagga
    13981 tccggatgat cggtcgggca accaactcat cgaggagtcg caggaagtcg gggtgcagcg
    14041 tgggctcgcc accggagagc atgacgacgt ccaggcgccc tcgctccttg gcgaggcgat
    14101 catcgacgga ggcgagcacc tccgcgaggg gcgccgtcgt tgcccgggcc ggcgccgact
    14161 cggcgaaaca cgtggggcag cgcaggttgc acgtgtcggt gatgtccgcg agcaggatgc
    14221 aggtgtgctg ggtctgcatg tgcggcatac cgtcgaggta tgccgcgggg accggcgcga
    14281 agttgcccat gacgtcggag acgtgttcct tggtcgtcgc ggtccactgc tcgaggtagg
    14341 tgaggatctc ggccgactcg tcatagagcg tccggacgaa tccgtgctcg gggcaaccgc
    14401 gttcgagcca gaccttgccg tcgcgcgcgg ccagccaccc cgacaggcga cgcacgtcgg
    14461 ccaggggctg gtcgggtcgc tcggcgtggc agtgggggca gaacgcgttg acgtaacggt
    14521 gaattcggaa gtctcgcaac ggcataccgg ggccgacgct cgggcgggca ctcacggcag
    14581 gctccctgcg agctgcgccg gagtatgccg cggcgcgagg acataggcgc cgcgccgcca
    14641 ctgccagacg acccgggcca ggaggatggg aacggtcgcg aggaggaaca gttgggagcg
    14701 gtccaggccg gcccagacgg cctcctcgcc gcgggtgaac tcgacgcaga accggaagac
    14761 gccgtatgcc gcgaggtaga cgacgagggt ctcgccgggc gcctgcatcc gatgccgaag
    14821 gactcgccaa aggacgacga aggcgatggc gtgaaaggcg atctcatagg cgtaggacgg
    14881 gtgcagaggc gttccggcga ccccgctgag ccgggctgct gtcgcgtcgt cgaggacggg
    14941 tccgaagccc agcgggttgg gtgagccggg gagctcggtg aacaggcagc cgaaccgccc
    15001 gatcgccatc ccgatggcca cggcgggggc gaacagatcg ccggtgcgcg gggagtagcc
    15061 ggccagccgc ttgctcacat gaacgccgag ccaggcaccg aacaggccgc caaggaccga
    15121 gcggttgcca tagacccatt gctgcgccag cgacgggttc gccccgagat cgaggtgctg
    15181 cagcacggga cccaggcgca tgaagatcgc cccgcccacg agggccccgg tgacgacgta
    15241 gagcaggcgg tagtcggcga cgccccgtcg acgcgtttcc acgatgaaga cggcgacggc
    15301 caccatcacc ccgaggccga cgaacgtgtc gtgggtcggc accgctagaa cgatgccgaa
    15361 cagatccccc agggttggaa acacggcccc acgctatccg agctctcgga acatgacgtg
    15421 cagcccgacc ggcccgtgat ggcggctgcg gaaggtcccc gggacggtgg tgaggattcg
    15481 gaagccgagt tgctgccaca gcgcgacggc gcccatgttc gtctcgacga ccgcgttgaa
    15541 ctgcatcgcg gcatagccct ggtcggtggc ccaccgcagg acctcctcgg cgagcgcgcg
    15601 gccgacgcct tgcccctggg cggtcggcga gaccatgaac gaggcggtcg cgatgtggtc
    15661 gccgcggccg ggccggttgg gcccggcttt ggcggtcccg aggatggtgc cgtccgcggc
    15721 atacgcgacg atgcagcgcg ccggcggagg ttccatccac gcggcggcga tctgcgcgtc
    15781 ggtgaggtct tcggggtagg cgtaggtctc gccgtcggcg acgatctcgc gaaagatcgg
    15841 ggcaatggcg gggaggtcat cgggcttcgc gtcgcggatc tgcacgttgg cagtatgccg
    15901 cgctagattc ccgagcatgg ctgactcgac gtccggcgct gatgacctcg tcctcgaagc
    15961 gaccgagcaa cgcgtcctcg ggagcctgct ggagaagcag gtcaccgtcc cctcgaccta
    16021 ccccatgtcg ctcaacgcga ttcggtcagc ctgcaaccag gccaccagtc gcgagccggt
    16081 cacggacttt gacgaggcga ccgtccagga gagtctgcgg ggtttgcggc tgcgtgagct
    16141 cgtgcgggtc gtgcatggcg accgggtcct gaagtatcac caactcctcg ggcagcggct
    16201 cgagctcgcc gacgacgagc gggccctcat caccgtgctg ttgctgcgcg gcccgcagtc
    16261 tccgggcgag ctcaagaccc gcaccgatcg gttgcacgcc tttgccgacc gcaccgaggt
    16321 cgaggagtgc ctggcgaggc tcgcctcgcg cgaggttccg ctggtggtcg agttgcccaa
    16381 ggcgcccggg catcacgacg ctcgctgggt gcatctgctc ggcccggtcg agttgcccgc
    16441 tgcggttgcg gccgcggtcg ctgctccggt cgtggaccga gaggtcgtgc tggccggcgg
    16501 cgcggcccag cgcgacgtga acgtgacggc ggcctacgac gcggtcgcga cggcatacgc
    16561 cctggagttc gggaccgagc tggaaggcaa gccgttcgac cgctggctgt tggcgcgagt
    16621 ggcgcagttg gcggggatcg acccggtggc cgacgtgggg tgcggcccgg ggcacgtcac
    16681 ccggttcctc gccgaggcgg gtgcttcggt gagcggattc gacgtgtccc cggggatggt
    16741 cgaggaggcg cgactgggat cgccggacct cggtttcgag gtcgcagact tccgtgggct
    16801 catgcgccct cggctcgctc ccgcgtgggg cgccatcgtc gcctggtatg ccgtcatcca
    16861 ccttgccgag tccgaactgg cttctgcggt ggctggattg gcgcgggtgc tgcgccccgg
    16921 aggctggctt gcgctggcca ctcatgtcgg cgacgcggtc caccatgtgg aggagttcct
    16981 cggggagccg gtgcagttgg acttcgtcct gcacgacgcc acggcggtgc gcgaagcggt
    17041 cgcggcggcc gg
//
LOCUS       DAORPW010000042        16569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_660_length_16569_cov_696.413891, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000042 DAORPW010000000
VERSION     DAORPW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 16569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16569
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_660_length_16569_cov_696.413891"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..759
                     /locus_tag="PLB86_05400"
     CDS             88..759
                     /locus_tag="PLB86_05400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC64603.1"
                     /translation="MDRPAQQNCYPDNAREDVRPFVPLDVSSVLEVGCGRGGFGRTLR
                     ELLGPGARLVAVEAVASSADAARDIGCFDEVVTGYFPEALTTPNERFDLVCFNDVLEH
                     VVDPAAALTATLDVLAQGGRVLATIPNVQYASVVYNLIRGRWTYTESGILDRTHLRFF
                     TRSSMAGLFHDAGYTVESTHGINPIGDVWQTDGNTPRRWLKSLIHPALGNARYLQFVL
                     VARRD"
     gene            complement(803..2176)
                     /locus_tag="PLB86_05405"
     CDS             complement(803..2176)
                     /locus_tag="PLB86_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRC64604.1"
                     /translation="MTDDILTPADVEAIRTRVRALLPTLRNDLEDLSRIPSVSLETLE
                     GYDQAHVDASAERVAALLRAEGLDVEIIREGGQPAVIGHIDGPPGAPTVTLYAHHDVQ
                     PAGDPALWDSDPWTPTEVDGRLYGRGVADDKAGVTAHLAALRAHAGNLPVGVTVFVEG
                     EEEVGSDSLPAILARHGHRLAADAIILADSGNWDIGLPALTTTLRGMLRAVVTVQTLD
                     HGVHSGMFGGACPDALTALIRLLATLHDEDGNVAVAGLRSGQAAALDYDEARLRRESG
                     LLDGVELIGSGPILDRLWTKPSMTTIGINAPTVENASNTLVPSASAKVSMRIAPDEDP
                     KTAYAALKAHLEAHVPWGAKLTVTLADDGPGFSATTDGPVYDQARAALADAFGVESVE
                     MGVGGSIPFVAAFAERFPEAAILVTGVEDPDTRAHGANESLHLGDWEKACLAEAIFLA
                     RLGALPR"
     gene            2249..3133
                     /locus_tag="PLB86_05410"
     CDS             2249..3133
                     /locus_tag="PLB86_05410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC64605.1"
                     /translation="MERETDFAVGREQWLDRLGNLRNVVRQEVIARQLARHVPDFSRM
                     RVMPGTDVPLPPPMRVLDVGAGQGTQAIRLARLGHRVTALEPDPRMRDVAAAALALEE
                     PGVRSRMDILAGGLGTLEAAVGWTEYDVVLCHGVFMYLPSAEPAVSELASHVASHGLL
                     SLVVRNAAGLALRPALQGRWDEVGVLLDEVAAAEEEGRDPLYTNEIGVRARADDVGTL
                     AEVCRVAGLLPVAHYGVRLVSDQVPLDTPTPTPEELEALVTTEVRLGDLDPYRQVCTL
                     AHLVFSPDLRDGLNRPNL"
     gene            3198..3773
                     /locus_tag="PLB86_05415"
     CDS             3198..3773
                     /locus_tag="PLB86_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HRC64606.1"
                     /translation="MFWSKKTQPAPVADDRPERPGAKNRPTPKRRDQEAANKHPVVNT
                     DRKAAARSDREARRVAMAESRAAMVTGDESKMPPRDKGPIKRYIRDYVDARFSLGEAL
                     LPMMLLALLLSFIKEVWALTTVFLLVYGLLFMSIADVFFMWRKIKAGVAAKFGADAMP
                     AGAMMYAVMRAYQIRRSRLPRPMIKRGEYPV"
     gene            complement(3833..5155)
                     /locus_tag="PLB86_05420"
     CDS             complement(3833..5155)
                     /locus_tag="PLB86_05420"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRC64607.1"
                     /translation="MNPQLQARIDHLVADRQRAWRAPNVSVGLVRDGQLVHTVHVGAA
                     RLDPVTAATDDTPFMIGSVTKTFTAVVIMALRDEGRLDLDDRLDAHLPGISHGELTIR
                     LMLGHLSGLQREPVGRVWESMDNPDADRLLAELAQAEQVLAPHEVFHYSNLAFALLGQ
                     VIERLERRPWEDVVRQRVLHPLGMTRTGLTPAADRARGYLVHPFSGVATEEPVADLKA
                     TAPMGGLWSTVADLSRYAAFVADPDPAVLAPATVATMCRPQVILDPDAWTLGYGLSFG
                     MVRRGDRIYVGHGGAMPGFLTGLRVSRPDRLGAVVWANSSAGAEPIVLAADLLDLVLD
                     AEPTAASPWTPEASVPELDEFLGSWWSEGEELVFSVRDGRLWCAPRGAGALFESRFIP
                     DGPDRFRVDLGRERGEALDVERDASGRISRMYLATYAVTREPLAFAEL"
     gene            complement(5152..5367)
                     /locus_tag="PLB86_05425"
     CDS             complement(5152..5367)
                     /locus_tag="PLB86_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64608.1"
                     /translation="MSTQWRWDLLDEAGNPMVGPEPASPSFPTQSEAESWIGEVWPEL
                     VDANIDAVTLREGETIVYGPMSLRPAP"
     gene            complement(5429..5941)
                     /locus_tag="PLB86_05430"
     CDS             complement(5429..5941)
                     /locus_tag="PLB86_05430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC64609.1"
                     /translation="MIASTPIELVQVDRSSRSVLENLGQLYRHDLSQGLAHLPNDDGT
                     FNNRRLDVFRSGADPTMRAWLIRVGGRLGGFVMTSTDEQSTHHIGDFFVVRALRRRGI
                     GHEVIRRVLADQPGPWVIGFQDDNPGAREFWTAVATEAVGDAWTLEHHPVPGLPHLPP
                     DSFIGFTVPG"
     gene            complement(6038..7501)
                     /gene="rpsA"
                     /locus_tag="PLB86_05435"
     CDS             complement(6038..7501)
                     /gene="rpsA"
                     /locus_tag="PLB86_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRC64610.1"
                     /translation="MSISTVTKTAPQVAINDIGSEEDLLAAIDATIKDFNDGDIVEGV
                     IVKVDRDEVLLDIGYKTEGVIPSRELSIKHDVDPADVVKVGDEVEALVLQKEDKEGRL
                     ILSKKRAQYERAWGTIEKVKEEDGVVTGTVIEVVKGGLILDIGLRGFLPASLVEMRRV
                     RDLQPYVGKQIEAKIIELDKNRNNVVLSRRAWLEQTQSEVRTTFLRELQKGQVRSGVV
                     SSIVNFGAFVDLGGVDGLVHVSELSWKHIDHPSEVVEVGNEVTVEVLDVDMDRERVSL
                     SLKATQEDPWQHFARTHAIGQVVPGKVTKLVPFGAFVRVDDGIEGLVHISELAERHVE
                     LPEQVVTVNEDIFVKVIDIDLERRRISLSLKQANDGTVGVADFDPTLYGMAAEYDEAG
                     NYKYPEGFDPETNEWLPGFEAQREKWEKQYAEAHARWEAHKAQVEAAAKADSEAAVAA
                     GDVPSSYSSSAAEAAPATGTLASDEALAALREKLTGN"
     gene            7715..8524
                     /locus_tag="PLB86_05440"
     CDS             7715..8524
                     /locus_tag="PLB86_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC64611.1"
                     /translation="MSAMDPLALRRDADIAETAAANRTWWDAEASDYYVEHGSFLGDD
                     DLVWGPEGWSEELLGLLGDVAGRDVLEIGGGAAQGGRWARQAGARVVSSDLSLGMLSR
                     GRAIDAAHDWAPLPLVQCDGLALPFAEASFDVVFTAYGVLPFVADSGRVLREVCRVLR
                     PGGRFVFSCTHPFRWAFPDEPDEAGLTVRFSYFDRTPYVEQDAQGRATYAEHHRTIGD
                     RVRDVVAAGLRVVDLVELEWPDGHEQEWGGWSPLRGRLIPGTLVMVADKPS"
     gene            complement(8567..9259)
                     /locus_tag="PLB86_05445"
     CDS             complement(8567..9259)
                     /locus_tag="PLB86_05445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRC64612.1"
                     /translation="MDLLLIRHAQSENNLVWAQTGASVGRVPDPLLTPLGEQQAVAVA
                     EALAQGRYAAPPTVLYASLMSRAVLTAVPIADALDLDVQGHPDLFEVGGPLDWSGHDD
                     VPRRAHRGAGRAALRELTPRLVLPDDAQPDGWWDGPVEADADAPERARRVVDSVLERH
                     GGTDEVVGLVTHGHFSQFLIRALLGIPSMTGWVDILNTSVSRFSNVDVPGRTSATWIN
                     RVAHLTPDAVTD"
     gene            complement(9348..12047)
                     /gene="polA"
                     /locus_tag="PLB86_05450"
     CDS             complement(9348..12047)
                     /gene="polA"
                     /locus_tag="PLB86_05450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRC64613.1"
                     /translation="MGSRLPYVSDTPRLLLLDGHSLAYRAFYALPAENFSTHTGQHTN
                     AVYGFTSMLINVLRDEAPTHIAVAFDVSRQTFRTEAYAEYKAGRSATPSEFEGQISLV
                     QEILGALRIPFVQVEGYEADDVIATLTSMATTQGMDVLICSGDRDAFQLVSDQVTVLY
                     PVRGVSELWRMTPAAVTEKYGVPPTRYSDLAALVGESSDNLPGVPGVGPKTAAKWIGM
                     YAGLDGIVANIDQIGGKAGESLREHLESVLRNRRLNQLVHDLPVGVELADLARASWDR
                     EQVHEVFDALEFRVLRERLFATFEAPEPEAESGFAVDGRVLGSGEVAGWLAQQAPAGV
                     RTGLQVLGRWGRGTGDARGLALATGAGAAAYLDLTGLDDVGEQALAAWLADPTRPKAI
                     HDGKGPAQALAARGLDLVGVTSDTALAAYLVKPDQRDYDLADLAVRLLRRELRDDTTD
                     TGQGMLDFGDGDNEANEAMVTARAVVDLADALDAELERTGGAALLHEVELPLVGVLAR
                     MERAGIAVDVEALRDLEADFATKVREAQEDAWEAIGRRDVNLGSPKQLQEVLFDQLGL
                     PKTKRTKTGYTTDADALTELYAKAEHPFLEALLRHRDAAKLRVTVEGLIKSVDDDGRI
                     HTTYLQTVAATGRLSSTDPNLQNVPIRTEEGRRIREMFVVGAGFESLMSADYSQIEMR
                     VMAHLSGDDGLIEAFRSGEDLHSFVASRVFGVEPTDVTLEMRSKIKAMSYGLAYGLSA
                     FGLSRQLGISTGEAKTLMDEYFVRFGEVRDYLHRLVADARGTGYTATMLGRRRYLPDL
                     LSDNRQRREMAERMALNAPIQGSAADIMKVAMLRVDEALRAGGFGSRMLLQVHDELVI
                     EVAPGERERVEELVRREMGAAVALEVPLEVSVGVGRTWHDAAH"
     gene            12046..12504
                     /locus_tag="PLB86_05455"
     CDS             12046..12504
                     /locus_tag="PLB86_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRC64614.1"
                     /translation="MTDSPPAAQDSPAETPWVTVEQVNAMAVGTLHERMGVTFTEISL
                     SRAVATMPVAGNTQPYGLLHGGASVVLAEGVGSFLAVVLAGPGRAAVGVDINATHHRP
                     VMSGTVTAVATPIAVGRTTASIEIVLTDEEGRRTCTCRFTAQLRDAPPQR"
     gene            complement(12506..12937)
                     /locus_tag="PLB86_05460"
     CDS             complement(12506..12937)
                     /locus_tag="PLB86_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRC64615.1"
                     /translation="MTRDLGTSIREDVVVLLARASGAALRTANAALEPFGFRARHYAT
                     VKIAAEGGGVPQRQIGAVLGLDPSAVVALVDDLESLGLVQRQPDPDDRRTRLVSPTTA
                     GLAMLETVSPVVGRVQERSMGGLTATERETFLHLLRRVIND"
     gene            13080..13988
                     /locus_tag="PLB86_05465"
     CDS             13080..13988
                     /locus_tag="PLB86_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC64616.1"
                     /translation="MDLSGKVAIVTGSGRGLGLAYAKALADAGASVVVNDIDEAVAQA
                     AADEITEAGGKAIAVAAAVGSSESAQALVDAAVAAFGRLDVLVANAGVLRDKVVWKMT
                     DEDFDLVTQVHLKGTFTCARAAIVRMKEQGEGGRLILIGSPAGQYGNFGQTNYAAAKA
                     GIVTFAKVWAMELSRDKITANAVVPVAATAMTATIPFLKPYVEAMQAGEPMPEFARRE
                     LAFGTPDDVAGLVVYLASDDSAGVNGQAIGIGGDRLSLYSHPDQIVKEYAAGGWSADA
                     IAAAWPEHFAPNAQSVGEKLPTPPSA"
     gene            14022..14894
                     /locus_tag="PLB86_05470"
     CDS             14022..14894
                     /locus_tag="PLB86_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC64617.1"
                     /translation="MSINVADLVAVDVHVHVEVDDHGHTALPQSFLDWSAKYFKSGGP
                     RTPTIDEIADRYRERKIAAVIFTVDVEKSMGQPRIPNEVVAEGAARNSDILIPFASID
                     PARGAEGVKELERLIADYGVKGIKFHPSLQNFAPNDGSAYGLFEVLAAHKLPAVFHTG
                     QTGIGAGMPGGGGVKLRYSDPMLLDDVAADFPDLPVILAHPSVPWQDQAISMATHKGN
                     VYIDLSGWSPKYFPPALVKAANGALRGKVLFASDFPVIDTDRWLADFETLDIKPEVRP
                     MILKDNAVRLLGLA"
     gene            14908..15363
                     /locus_tag="PLB86_05475"
     CDS             14908..15363
                     /locus_tag="PLB86_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRC64618.1"
                     /translation="MTTTVHFDDLASLIGVPLGATEWLTITQDRINTFADATGDPQWI
                     HVDPERAKSGPFGTTIAHGYLTLSLIVPLFVDLLDVEGVTTKLNYGLNKVRFPAPVKV
                     DSRIRLVAHITEVEVVGGGGKQITWAGTVEIEGSDKPACVAEPVYRFYA"
     gene            15447..>16569
                     /locus_tag="PLB86_05480"
     CDS             15447..>16569
                     /locus_tag="PLB86_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC64619.1"
                     /translation="MSHYRSDRTHSQFLLFDVFGVDRAYGVEPYEDFDPETAAEILEQ
                     VEAFAHDKLADSFPSADVHPIRLDPVRHTVTLPRDLRASLAAHVASDWLRLDLPADVS
                     GIHVPASLRWAATEFILGANPAVAMCSQLIPTVVAMLHRNGNKEQQRLAELILERNWT
                     VTMVLTEPDAGSDVGLGRTRAALADDGNWHLTGTKRFITWAAHDAAENVIHCVLARPQ
                     GVEGQGGPGTKGLSLFMVPSQLFDPETGELLGPNGVVAANIEHKMGILGTPTCELVLG
                     NREPAVGTLLGDTHDGIRQMFEIITYVRMMVGLKTVSALSSGHLNARDYAMSRKQGRE
                     LISLTGARGGKPLVEIVDHPDIRRSLLTQKAYAEGGRALV"
BASE COUNT         2585 a         5806 c         5612 g         2566 t
ORIGIN      
        1 accccgagat cgcccactgg gcgcgagtcg ccctcgacca gatgctcgcg cttcccggcc
       61 ccacccacaa ggactgacga ggccttcgtg gaccggccgg cccagcaaaa ctgttacccc
      121 gacaacgcgc gcgaggacgt ccgcccgttc gtccccctgg acgtcagctc ggtgcttgag
      181 gtcggctgcg gccgtggagg attcggccgc accctgcggg agctgctcgg gcctggggca
      241 cgcctcgtgg cggtggaagc agtcgcctcg tccgccgacg ctgcgcgcga catcggctgc
      301 ttcgacgagg tcgtcaccgg atacttcccc gaggcgctga cgacgcctaa cgaacgcttc
      361 gacctcgtgt gtttcaacga tgtgctggag cacgtggtcg accctgcggc ggcgctcacc
      421 gcgacgttgg acgtcctcgc tcagggtggt cgagtgctgg ccacgattcc caacgtccag
      481 tacgccagcg tcgtctacaa cctcatccgc ggtcggtgga cctacaccga gtcaggcatt
      541 ctggaccgga ctcacctgcg gttcttcacc cgatcgagca tggccgggct gttccacgat
      601 gcgggctata ccgtggagtc gacgcacggc atcaacccca tcggcgacgt ctggcagacc
      661 gatgggaaca ctccgcgccg ctggctcaag tcgctgatcc atccggcact gggtaacgcg
      721 cggtaccttc agttcgtgct ggtggctcgc cgcgactaag ggctgcgctc aacgggcagg
      781 actccctacc ggacggcaga gctcagcggg gcagggcacc caacctggcc aggaagatcg
      841 cctccgcgag acacgccttc tcccaatcgc ccaggtggag tgactcgttg gccccgtgtg
      901 ctcgcgtgtc ggggtcctcg accccggtga cgaggatcgc ggcctcgggg aagcgctcgg
      961 cgaaggcggc cacgaagggg atcgatcctc cgacacccat ctcgaccgat tcgacgccga
     1021 aggcatccgc caaggcggcc cgggcctggt cgtagaccgg cccatcggtc gtcgcggaga
     1081 agcccggccc gtcgtcggcg agggtgacgg tgagcttggc cccccacggg acgtgcgcct
     1141 ccaggtgagc cttgagcgcg gcatacgccg tcttggggtc ctcgtcgggg gcgatccgca
     1201 tggagacctt cgccgaggcg ctcggaacga gcgtgttgga ggcgttctcg accgtgggag
     1261 cgttgatgcc gatcgtcgtc atcgacggct tggtccagag ccggtcgagg atggggccgc
     1321 tgccgatcaa ctcgacacca tccaacaggc ccgactcacg ccgcaaccgg gcctcgtcgt
     1381 agtccaacgc agccgcctgg cccgaccgca gccccgcgac cgcgacgttg ccgtcctcgt
     1441 cgtgcagggt ggccaggagc cggatgaggg ccgtgagggc gtcggggcag gccccgccga
     1501 acattcccga gtggacgccg tggtcgaggg tttggacggt gacaacggca cgaagcatgc
     1561 cgcgcagcgt cgtcgtgagg gccggcaggc cgatgtccca gttgcccgag tcggcgagga
     1621 tgatcgcatc ggcggccagg cggtggccgt ggcgggcgag gatggcgggc agcgagtcgg
     1681 acccgacctc ctcctcgccc tcgacgaaga cggtcacccc gaccggcaag ttgccggcgt
     1741 gggcgcgcag cgcggccagg tgggccgtca cgccggcctt gtcgtcggcc acgccacgcc
     1801 cgtagagccg gccgtcgacc tccgtcggtg tccaggggtc gctgtcccag agcgcggggt
     1861 caccggcggg ctggacatcg tggtgggcgt aaagcgtcac cgtcggggcg ccgggcggcc
     1921 cgtcgatgtg accgatcacc gccggttgac cgccctcgcg gatgatctcg acgtcaagcc
     1981 cttcggcccg cagcaacgcc gcgacccgct cggcgctcgc atcgacgtgc gcttggtcgt
     2041 aaccctcgag cgtctccaac gagacgctcg ggatgcggga caggtcttcc aggtcgttgc
     2101 gcagggtggg caacagggcg cggacgcggg tacggatggc ctcgacgtcg gcgggagtga
     2161 gaatgtcatc agtcacccgc gtgagcctat ccacgccgga cggcgacgcg cgtgggacgg
     2221 tggagacttg ttggcccgag gaggcctgat ggagcgagaa accgacttcg cggtcggccg
     2281 cgagcagtgg ttggaccgac tgggcaacct gcgcaacgtc gttcgccagg aagtcatcgc
     2341 ccgccaactc gcccggcacg ttcccgactt ctcacgcatg cgggtcatgc cagggacgga
     2401 cgtgccgctg ccgccaccga tgagggtcct tgacgtcggt gccggtcaag gcacccaggc
     2461 gatccggctg gcccgactcg ggcaccgggt gactgcgctc gagcccgacc cgcggatgcg
     2521 cgacgtggcg gcggccgccc tagctctgga ggagcctggg gtgcgctcgc ggatggatat
     2581 cctcgcgggc ggtctcggca ccctggaggc cgcggtgggc tggactgaat acgacgtcgt
     2641 gctgtgccac ggcgtgttca tgtacctccc ctccgcggag cctgctgttt cggagctcgc
     2701 gtcgcacgtc gcctcacatg gcctgctctc actggtcgta cgcaacgcgg ccgggctggc
     2761 cctgcggcct gcgttgcagg gtcgatggga cgaggtcggc gtgttgctgg acgaagttgc
     2821 tgccgcggag gaagagggcc gagatccgct ctacaccaac gagatcgggg tccgcgctcg
     2881 ggcggatgac gtgggcacac tggccgaggt atgccgcgtg gccggcctgc tcccggtcgc
     2941 tcactacggg gtccggctgg tctccgacca ggtgcccctc gacaccccga ctccaacccc
     3001 ggaagagctt gaggctctgg tgaccaccga ggtgcgcctc ggcgatctcg acccctaccg
     3061 gcaggtgtgc accttggccc atctggtctt ctcgccggac ctccgagacg ggctgaaccg
     3121 cccaaacctg tgaaccggac cctccgacag ttgtcggacg gacgggtgcc gcggctggac
     3181 ggcatacagt tgccctcgtg ttctggagca agaagacgca gcccgccccc gtcgccgacg
     3241 accgccccga gcgtcccggc gccaagaacc gacccacgcc caagcggcgc gaccaggagg
     3301 cagccaacaa gcacccggtc gtcaacaccg accgcaaggc ggcggcccgc agtgatcgcg
     3361 aggcccgccg ggtggcgatg gccgagtcgc gagccgcgat ggtcaccggc gacgagtcga
     3421 agatgccgcc ccgcgacaag ggccccatca agcgctatat ccgcgactac gtcgacgccc
     3481 ggttcagcct cggcgaggcc ctgttgccga tgatgctcct cgcgctgctc ctgtcgttca
     3541 tcaaagaggt ctgggcgctg accaccgtct tcctgctggt ctacgggttg ttgttcatgt
     3601 cgatcgccga cgtgttcttc atgtggcgca agatcaaggc gggggtggcg gcgaagttcg
     3661 gcgccgacgc gatgcccgcc ggcgcgatga tgtatgccgt gatgcgcgcc taccagatcc
     3721 ggcgcagccg actgccgcgc ccgatgatca agcggggcga atacccggtc tgacctgggc
     3781 tgcgtggact cacctggact gaccgatctg acccggtttg gcgctcggcg cctcagagtt
     3841 cggcgaaggc cagcggttcg cgggtgacgg cgtaggtcgc gagatacatc cgggagatcc
     3901 ggccgctcgc gtcgcgctcc acgtcgaggg cttcgccgcg ctcgcgaccg aggtcgacgc
     3961 ggaagcggtc aggcccgtcg gggatgaacc ggctctcgaa cagcgccccc gcgccacgcg
     4021 gcgcgcacca gaggcgcccg tcgcggacgg aaaacaccaa ctcttcgccc tcggaccacc
     4081 acgacccgag gaactcgtcg agctccggca ccgacgcctc gggcgtccag ggggatgccg
     4141 ccgtgggctc ggcatcgagg accaggtcga gcaggtcggc ggccaggacg atgggttcgg
     4201 caccggctga ggagttggcc cacaccacgg cgccgagccg atcaggccga ctgacccgaa
     4261 gcccggtgag gaagcccggc atcgctcccc cgtgcccgac gtagatgcgg tcgccccgtc
     4321 ggaccatccc gaaggacaag ccatatccca gcgtccatgc gtcggggtca aggatgacct
     4381 gcggccggca catcgtggcc actgtggccg gcgcgaggac ggccgggtcg gggtcggcga
     4441 cgaacgcggc ataccggctg aggtcggcga ccgtcgacca gagcccgccc atgggggccg
     4501 tggccttgag gtcggccacc ggctcctcgg tggccacccc ggagaagggg tgaacgaggt
     4561 agccgcgcgc ccggtcggcc gccggagtga gccccgtgcg ggtcatcccc agcgggtgta
     4621 ggacgcgctg ccggacgacg tcttcccagg ggcggcgttc gagccgctcg atcacctggc
     4681 caagcagggc gaaggcgaga ttggagtagt gaaagacctc atgcggtgcc agcacctgct
     4741 ccgcctgcgc caactcagcg agcaaccggt cggcatcggg gttgtccatg ctctcccaga
     4801 cccgaccgac gggctcgcgc tgcagaccgg acagatgccc caacatgagg cggatcgtca
     4861 gctctccgtg ggagattccg ggcaggtgag catcgagccg atcatcgaga tccagacgcc
     4921 cctcgtcgcg caacgccatg atcaccacgg ccgtgaaggt cttggtgacc gacccaatca
     4981 tgaacggtgt gtcgtcggtg gcggccgtga ctgggtccaa ccgggcggcg ccgacatgca
     5041 cggtgtggac gagctgcccg tcgcgcacca gcccgaccga gacgttcgga gcacgccacg
     5101 cccgttgccg gtcggcgacg agatggtcga tgcgggcttg aagctgcggg ttcacggggc
     5161 gggtcgcagc gacatcgggc cgtagacgat cgtctcgccc tcgcgcaagg tcaccgcgtc
     5221 gatgttggcg tcgacgagct caggccagac ttcaccgatc cacgactcgg cctcggattg
     5281 ggtggggaac gacggagagg ccggctcagg tccgaccatg gggttcccgg cctcgtcgag
     5341 gaggtcccag cgccactgcg tgctcatagc ccccacgcta acccacgggg tgggcgctgg
     5401 aatgcgcgat ggcgagccct ccacgtgctc agccggggac cgtgaacccg atgaacgagt
     5461 ccggcggcag gtgcggaagg cccggcacgg ggtggtgttc caacgtccac gcatctccga
     5521 ccgcctcggt ggcgaccgcg gtccagaact cccgcgcgcc cgggttgtcg tcctggaagc
     5581 cgatcaccca tggccccggt tgatcggcga ggacccgacg gatcacctcg tgcccgatgc
     5641 cccgacgccg tagcgcccgg acgacgaaga agtcgccgat gtggtgggtg ctttgctcat
     5701 cggtgctcgt catgacgaag ccacccaacc ggccgcccac ccggatcagc caggcccgca
     5761 tcgtggggtc ggcgccggac cggaacacgt ccaggcgccg gttgttgaag gtgccgtcgt
     5821 cgttcggcag gtgggccagg ccttgggaga ggtcatgccg atagagctgc ccgaggtttt
     5881 ccaggaccga ccgagagctg cggtcgacct ggacgagctc gatgggtgtc gacgcgatca
     5941 ccgagggagc ataacgaagg gcccggacca cacgcggtgg tccgggccct gtcgtgaacc
     6001 ggagcgggcg gcatacccag tcggtatgcc gcgtcgctca gttgccggtg agcttctcgc
     6061 gcaacgcggc cagggcctcg tcggacgcga gcgtgccggt ggccggagcg gcctcggcgg
     6121 cggaggagga gtaggacgac gggacgtcgc cggccgcgac cgcagcctcg gagtcggcct
     6181 tggcagccgc ctcgacctgc gccttgtggg cctcccagcg ggcgtgggcc tcggcgtact
     6241 gcttctccca cttctcgcgc tgagcctcga agcccggcag ccactcgttg gtctccgggt
     6301 cgaagccctc ggggtacttg tagttgcccg cctcgtcgta ctcggcagcc atgccgtaga
     6361 gggtcgggtc gaagtcggcg acaccgaccg tcccgtcgtt ggcctgcttg agcgacaacg
     6421 agatccggcg acgctcaagg tcgatgtcga tgaccttgac gaagatgtcc tcgttgaccg
     6481 tgacgacctg ctcgggcagc tcgacgtgac gctcggccag ctcggagatg tggaccaggc
     6541 cctcgatgcc gtcgtcgacg cgcacgaacg caccgaacgg gacgagcttg gtgaccttgc
     6601 ccggcacgac ctgaccgatc gcgtgcgtcc gggcgaagtg ctgccacggg tcttcctgcg
     6661 tcgccttgag cgacaaggac acgcgctcgc ggtccatgtc gacgtcgagg acctcgacgg
     6721 tcacctcgtt gccgacctcg acaacctcgc tcgggtggtc gatgtgcttc caggacagct
     6781 cggagacgtg gaccagaccg tcaaccccac cgaggtcgac gaacgcaccg aagttgacga
     6841 tgctcgacac gacaccggag cggacctggc ccttctgcag ctcgcgcagg aaggtcgtgc
     6901 ggacctcgga ctgggtctgc tcgagccacg cgcggcgcga caggacgacg ttgttgcggt
     6961 tcttgtccag ctcgatgatc ttggcctcga tctgcttgcc gacatacggc tgcaggtcgc
     7021 ggacgcggcg catctcgacg agggaggcgg gcaggaagcc acgcaggccg atgtccagga
     7081 tcaggccgcc cttgacgacc tcgatgacgg tgccggtgac gacgccatcc tcttccttga
     7141 ccttctcgat cgtgccccag gcgcgttcgt actgtgcgcg cttcttggac aggatgagcc
     7201 gaccctcctt gtcctccttc tggagaacga gagcttcgac ctcgtcgccg accttgacca
     7261 catcggccgg gtcgacgtcg tgcttgatgg acagctcacg cgaggggatg acgccctcgg
     7321 tcttgtagcc gatgtcgagc aggacctcgt cccggtcgac cttgacgatg acaccctcga
     7381 cgatgtcgcc gtcgttgaaa tccttgatcg tcgcgtcaat cgcggcgagg aggtcttcct
     7441 cagagccaat gtcgttgatg gcaacctgag gggcggtctt ggtgaccgtg ctgatgctca
     7501 tgtagtagga actccgatgg tggacaggat gtaaagccgc acgccggtcg gcctgcgggg
     7561 cggacatggt tgggtgttcg ggtctgacga ctcgccggga gcgggcaggc agcatcgaac
     7621 cgcgccccga agggcactgg gtcagggtac ccgcccgcgc gtggccgggt caaagctgtt
     7681 cagtatgccg tcccgccgcg acgacccggc cagaatgagc gccatggatc ccctcgcgtt
     7741 gcgccgcgat gccgacatag ccgagaccgc ggcggccaac cgcacctggt gggatgccga
     7801 ggcgagcgac tactacgtcg aacacggttc cttcctgggc gacgacgacc tggtctgggg
     7861 gccggaaggg tggagcgagg aactcctggg gttgcttggt gacgtcgccg ggcgcgatgt
     7921 tctggagatc ggcggcggcg cagcacaagg tggccgctgg gctcgccagg ctggggcccg
     7981 ggtggtgtcg agtgacctct ccctgggcat gctgtcgcgg ggacgggcca tcgacgcggc
     8041 ccacgactgg gctcccctcc cgctggtcca gtgcgacggg cttgccctcc ccttcgccga
     8101 ggcgagcttc gacgtcgtgt tcacggcata cggagtcctg ccgttcgtcg ccgactccgg
     8161 tcgggtcctg cgcgaggtat gccgggtgct tcggcccggg ggccggttcg tcttctcgtg
     8221 cacgcacccg ttccggtggg cgttccccga cgagccggac gaggcgggcc tgacggtgcg
     8281 gttctcctac ttcgaccgga cgccctatgt cgagcaggac gcgcaggggc gggcgaccta
     8341 cgccgagcac caccgaacca tcggcgaccg ggtgcgggac gttgtcgcgg ccggtctgcg
     8401 cgtcgtggac ctcgtcgagt tggaatggcc cgacggtcat gagcaggaat ggggtggctg
     8461 gtcgccgcta cgtggccggc tcatccccgg caccctcgtc atggtcgccg acaagcccag
     8521 ctagggcagg tagaccaggt agaccggccg gccccgcgac cagcgctcag tcggtcacgg
     8581 cgtcgggggt cagatgcgcc acgcggttga tccacgtggc cgacgtccgc cccggcacgt
     8641 caacgttgga gaaccgggac accgaggtgt tgaggatgtc cacccacccg gtcatggagg
     8701 ggatgcccag cagcgcccgg atgaggaact ggctgaagtg cccgtgggtg accagcccga
     8761 ccacctcatc ggtgccgccg tggcgttcga gcaccgagtc gaccactcgg cgagcccgct
     8821 cgggggcgtc ggcgtcggcc tccactggac cgtcccacca cccgtcgggt tgggcgtcgt
     8881 caggcagcac caggcggggg gtcagttcgc ggagcgccgc tcgccccgcc ccgcggtgag
     8941 cccggcgcgg cacgtcgtca tggccgctcc agtccagtgg tccgccgacc tcgaacaggt
     9001 cgggatgccc ctgcacgtca agatccagcg cgtcggcgat cggcacggcc gtgaggaccg
     9061 cccgactcat caggctcgcg tagagaacgg tgggcggggc ggcataccgg ccctgtgcga
     9121 gcgcctcggc gaccgccacc gcctgctgct cccccaacgg ggtgagcagc gggtcaggca
     9181 cccgcccgac ggaagcgccc gtctgcgccc acacgaggtt gttctccgac tgcgcatgtc
     9241 tgatgagcag cagatccatg tatgccgttc ccctcgccgc tcgtgaccgt gatccgtgac
     9301 gcgtaatcct ggtccgtgat ccgtgatccg tggtatctga agctgcctca gtgtgccgcg
     9361 tcgtgccagg tgcggccgac gccgaccgac acttccagcg gcacctcgag ggcgaccgcg
     9421 gcgcccattt cacgccggac caactcctcg acccgctccc gctcgccggg ggccacctcg
     9481 atgaccaact cgtcgtggac ctgcaggagc atccgcgacc cgaacccacc ggcccgaagc
     9541 gcctcgtcga cccgcaacat ggcgaccttc atgatgtcgg cggccgagcc ttggatcggg
     9601 gcattgagcg ccatccgctc ggccatctcg cggcgctggc ggttgtccga gagcaggtcg
     9661 ggcagatacc gccgccgacc gagcatcgtc gcggtgtaac ccgtgccgcg agcgtcggcg
     9721 acgagccgat gcaggtagtc gcggacttct ccgaagcgca cgaagtattc gtccatcagg
     9781 gtcttggcct cgccggtgga gatccccaac tggcgggaga gcccaaaggc cgacaggccg
     9841 taggccaggc cgtaggacat cgccttgatc ttggaacgca tctccagcgt gacgtcggtc
     9901 ggctcgacgc cgaacacccg cgacgccacg aaggagtgca ggtcctcccc cgagcggaac
     9961 gcctcgatca gcccgtcgtc gccggacagg tgggccatca cccgcatctc gatctggctg
    10021 tagtccgccg acatgagcga ctcgaacccg gcgcccacga cgaacatctc ccggatccgg
    10081 cgcccctcct cggtgcggat cgggacgttc tgcaggttgg ggtcggtcga gctcagccga
    10141 ccggtcgccg cgaccgtctg caggtaggtc gtgtggatcc gaccgtcgtc atcaacgctc
    10201 ttgatgagtc cttcgaccgt gacccgcagt ttggccgcgt cgcgatggcg cagcagcgcc
    10261 tcgaggaacg ggtgctcggc cttggcgtag agctcggtca aggcgtcggc atcggtcgta
    10321 taaccggtct tggtgcgctt ggtcttgggc aggcccaact gatcgaagag cacctcctgc
    10381 aactgcttgg ggctgccgag gttgacgtcg cgacgcccga tcgcctccca agcgtcctcc
    10441 tgcgcctcgc gcaccttggt cgcaaagtcg gcctccaggt cacgcagcgc ctcgacgtcg
    10501 acggcgatgc cagcgcgctc catccgggcg agcacgccga ccagcggcaa ctcgacctca
    10561 tgcagcagtg ccgccccgcc cgtgcgctcc aactcggcgt cgagcgcgtc ggcgaggtcg
    10621 acgaccgcgc gcgcggtgac catcgcttcg ttcgcctcgt tgtccccgtc tccgaagtcg
    10681 agcatcccct gcccggtgtc ggtggtgtcg tcgcgcaact cccgccgcag cagtcgcacc
    10741 gcgaggtcgg ccaggtcgta gtcgcgttgg tcgggtttga cgaggtatgc cgccagcgcc
    10801 gtgtcgctcg tcacgccgac aagatcgagc ccgcgagccg ccagggcctg cgccgggccc
    10861 ttgccgtcgt ggatcgcctt ggggcgggtg gggtcggcca gccaggcggc cagcgcctgc
    10921 tcgcccacgt cgtcgagccc ggtgagatcg aggtatgccg ccgcgccggc tcccgtggcc
    10981 agggccagcc cgcgcgcgtc gccggtcccc cgcccccagc ggccgaggac ctgcagccca
    11041 gtgcggaccc cggccggggc ctgctgcgcc agccagccgg ccacctcgcc tgagccgagc
    11101 acccgtccgt cgacggcgaa gcccgactcg gcctccggct ccggagcctc gaaggtcgcg
    11161 aacaggcgct cccgcagcac ccggaactcc aaggcgtcga agacctcatg cacctgctcg
    11221 cgatcccacg aggctctggc caggtcggcc aactcgaccc cgaccggcag gtcgtggacg
    11281 agttggttga gtcggcggtt gcgcagcacc gactccaggt gctcgcgcag cgactctccg
    11341 gccttcccgc cgatctggtc gatgttcgcg acgatcccgt cgaggccggc atacatcccg
    11401 atccacttgg ccgcggtctt ggggccgacg ccggggacgc cggggaggtt gtccgaggac
    11461 tcgccgacga gcgcagcgag gtcgctgtag cgcgtgggcg ggacaccgta tttctcggtc
    11521 accgccgctg gggtcatccg ccacaactcc gacactcccc gcacggggta gagcacggtg
    11581 acctggtcgg agacgagttg gaaggcatcg cggtcgcccg agcagatgag gacgtccatg
    11641 ccttgcgtcg tggccatcga cgtcagggtg gcgatgacgt cgtccgcttc atacccctcg
    11701 acctggacga aggggatgcg cagcgccccg aggatctctt ggacgagcga gatctgcccc
    11761 tcgaactcgc tcggggtcgc cgaccggccg gccttgtatt cggcgtaggc ctcggtgcgg
    11821 aaggtctgac gggacacgtc gaaggccacc gcgatgtggg tcggcgcctc gtcacgcagg
    11881 acgttgatga gcatcgaggt gaagccgtag acggcgttgg tgtgctgccc ggtatgggtc
    11941 gagaagttct ccgcgggcaa cgcgtagaac gcgcgatagg ccaacgaatg accgtccagc
    12001 agcagaaggc gaggcgtgtc tgacacgtat ggcagcctag agcccatgac cgacagcccc
    12061 cccgccgcgc aggattcccc cgccgagacg ccgtgggtca cggtcgagca ggtcaacgcg
    12121 atggccgtcg gcaccctgca cgagcggatg ggcgtcacct tcaccgagat ctcgctgtcc
    12181 cgcgcggtgg ccaccatgcc ggtcgcgggc aacacccagc cctacggtct gctgcacggt
    12241 ggcgcctcgg tggtgcttgc cgaaggggtc gggtccttcc tcgcggtcgt cctggccggg
    12301 ccgggccggg cggccgtcgg cgtcgacatc aatgccaccc accatcggcc ggtgatgtcc
    12361 ggcaccgtca ccgccgtggc caccccgatc gccgtcggtc gcacgacggc ctcgatcgag
    12421 atcgtcctca cggacgagga gggtcggcgc acctgcacgt gccggttcac ggcccagctg
    12481 cgcgacgcgc cgccgcagcg ctgaactagt cgttgatgac gcgacgcaac aggtgcaaga
    12541 aggtctcccg ctcggtggcg gtcaagccac ccatggaacg ctcctgcacc cgacccacga
    12601 ccggcgacac cgtctcaagc atggccagac cggcagtcgt cggtgagacc agtcgggtgc
    12661 ggcggtcgtc cgggtcgggt tggcgctgca ccaggccgag ggactcaagg tcatcgacga
    12721 gggcgacaac ggcgctcgga tccaggccca ggacggcgcc gatctgacgc tgcgggacgc
    12781 caccgccctc ggcggcgatc ttcaccgtgg cgtagtgacg ggcccggaaa ccgaacggtt
    12841 cgagagccgc gttcgcggtg cgcagtgccg caccggacgc gcgtgccaac aggaccacga
    12901 cgtcctcccg gatgctcgtg cccaggtcgc gtgtcatacc ccccattgtg gcacgtacgt
    12961 cacaatcttc aatgattgac ttctacaatc gttgtgactt atacatacct cgtgggcgtg
    13021 gcgccgacgc caggcgctcc atgccccctc atgcaatgcc cagccacgaa ggagtagcaa
    13081 tggacctgtc cggcaaggtc gcgatcgtca ccggaagcgg gaggggcctg gggctcgcct
    13141 acgccaaggc tctggccgac gcgggtgcct ccgtcgtcgt caacgacatc gacgaagctg
    13201 tggcccaggc cgctgccgac gagatcaccg aggccggggg taaggccatt gcggtcgccg
    13261 ccgccgtcgg atcgtccgag tccgcccagg ccctcgtcga cgctgcggtc gccgccttcg
    13321 gccgactcga cgtcctggtc gccaacgccg gcgtcctgcg cgacaaggtc gtctggaaga
    13381 tgaccgacga ggacttcgac ctcgtcaccc aggtccacct caagggcacg ttcacctgcg
    13441 cccgcgcggc catcgtgcgg atgaaggagc agggcgaagg cggtcggctc atcctcatcg
    13501 gctcccccgc cggccagtac ggcaacttcg gccagaccaa ctacgcggcc gccaaggccg
    13561 gcatcgtcac cttcgccaag gtgtgggcca tggagctctc gcgcgacaag atcacggcca
    13621 acgccgtcgt ccccgtcgcc gccaccgcga tgaccgccac catcccgttc ctcaagccct
    13681 atgtcgaggc gatgcaggcc ggcgagccga tgcccgagtt cgcccggcgc gagctggcgt
    13741 tcggcacccc tgacgacgtc gccgggctgg tcgtctacct cgcctccgat gactccgccg
    13801 gggtcaacgg acaggccatc ggcatcggcg gcgaccgcct ctcgctctac tcccaccccg
    13861 accagatcgt caaggagtat gccgccggcg gctggtccgc tgacgccatc gccgccgcgt
    13921 ggccggagca cttcgcgccc aacgcgcagt cggtcggcga gaagctcccg actcccccgt
    13981 ccgcctgatc acccagccac ccggcatacc aaggagaagt gatgagcatc aacgtggccg
    14041 acctggtcgc cgtcgacgtg cacgtccacg tcgaagtgga tgaccatgga cacacggccc
    14101 tgccgcagtc cttcctcgac tggtcggcga agtacttcaa gtccggcggc ccgcgcaccc
    14161 cgacgatcga cgagatcgcc gatcgctacc gcgagcgcaa gatcgcggcc gtgatcttca
    14221 cggtcgacgt cgagaagtcg atgggccagc cgcgcatccc caacgaggtc gtcgccgagg
    14281 gcgctgcgcg caactccgac atcctcatcc ccttcgccag catcgacccc gctcgcgggg
    14341 ctgaaggcgt caaggagttg gagcgcctca tcgccgacta cggcgtcaag ggcatcaagt
    14401 tccaccccag ccttcagaac ttcgccccca acgacggcag cgcctacggc ctcttcgagg
    14461 tgctcgccgc ccacaaactg ccggccgtgt tccacaccgg gcagaccggg atcggcgcgg
    14521 gtatgccggg tggcggcggc gtgaagctgc gctacagcga ccccatgttg ctggacgacg
    14581 tcgcggccga cttccccgat ctcccggtca tcctcgcgca cccgtcggtg ccgtggcagg
    14641 accaggcgat ctcgatggca acccacaagg gcaacgtcta catcgacctg tccgggtggt
    14701 cgccgaagta cttcccgccg gccctcgtca aggccgccaa cggcgcgctg cgtggcaagg
    14761 tgctcttcgc ctcggacttc ccggtcatcg acaccgatcg ctggctggcc gactttgaga
    14821 cgctcgacat caagcccgag gtccgaccga tgatcctcaa ggacaacgcg gtccggctgc
    14881 tcggcctggc ctgaaaagga gctcacgatg acgactacgg tgcacttcga cgacctggcc
    14941 tccctgatcg gcgtcccgct cggcgccacc gagtggctga cgatcaccca ggaccggatc
    15001 aacaccttcg ccgacgcgac cggtgacccg caatggatcc acgtcgaccc cgagcgggcc
    15061 aagtctggcc ccttcgggac gacgatcgcc catggctacc tcaccctgtc actgatcgtc
    15121 ccgctgttcg tcgacctgct cgacgtcgag ggagtgacga ccaagctcaa ctacgggctc
    15181 aacaaggtcc gcttccccgc ccccgtcaag gtcgacagcc gcatccggtt ggtcgcccac
    15241 atcaccgagg tcgaggtagt cggcggcggc ggcaagcaga tcacctgggc cggcaccgtc
    15301 gaaatcgagg gctcggacaa gccggcctgc gtcgccgagc cggtctaccg cttctacgcc
    15361 tgaccgtcag acggtggcca cccgcacccg ggtggccacc gtctgcggcc ctgtccgccc
    15421 gtcccgcgct cgacgaggag cccctgatga gccactaccg cagtgaccgc acgcactccc
    15481 agttcctgct cttcgacgtg ttcggcgtcg accgggccta cggcgtcgag ccctatgaag
    15541 acttcgatcc cgagacggcc gcggagatcc tcgagcaggt cgaggccttc gcgcacgaca
    15601 agctggctga ctccttcccg tcggccgacg tgcacccgat ccggctcgac ccggtgcgcc
    15661 acacggtgac cctcccccgc gacctgcgcg cgtcgctggc cgcccacgtc gcgtcggact
    15721 ggctccgact ggacctcccg gccgacgtat cgggcatcca cgtgcctgcc tcattgcgct
    15781 gggccgcaac ggagttcatc ctcggcgcca atccggccgt cgccatgtgc agtcagctca
    15841 tccccaccgt cgtcgcgatg ttgcaccgca atggcaacaa ggagcagcag cgactcgccg
    15901 agctcatcct cgagcgcaat tggaccgtga cgatggtgct caccgagccc gatgcgggct
    15961 ccgacgtcgg gctcgggcgc acccgcgcgg ccctcgccga cgacgggaac tggcacctca
    16021 cggggaccaa gcgcttcatc acctgggccg ctcacgacgc ggctgagaac gtcatccact
    16081 gcgtccttgc gcgcccgcag ggcgtcgagg ggcagggcgg cccggggacc aagggtctgt
    16141 cgctgttcat ggtcccgtcg cagttgttcg acccggagac tggggagttg ctgggtccca
    16201 atggggtggt cgccgccaac atcgagcaca agatgggcat cctcggcacc ccgacttgcg
    16261 agctcgtcct cggcaaccgc gagcccgcgg tggggaccct cctcggcgac acgcacgacg
    16321 gcatccggca gatgttcgag atcatcacct acgtccggat gatggtcggc ctcaagacgg
    16381 tctccgccct ctccagcggg catctcaatg cccgcgatta tgcgatgagt cgcaagcagg
    16441 ggcgcgagtt gatctcgttg accggggctc gcggcgggaa gccgctggtg gagatcgtcg
    16501 accaccccga catccgtcgg tcgctgctca cccagaaggc ctatgccgag ggtggccgtg
    16561 ccctcgtcc
//
LOCUS       DAORPW010000043        16560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_661_length_16560_cov_736.706756, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000043 DAORPW010000000
VERSION     DAORPW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 16560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16560
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_661_length_16560_cov_736.706756"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLB86_05485"
     CDS             complement(<1..369)
                     /locus_tag="PLB86_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRC64620.1"
                     /translation="MSEPVLALDIGGTKIAAALVTGGHVGEVCRVATPASQGPEAVVA
                     AAVAVAKEARGESRPVLVGIGSAGVIDSTNGVVTHATDALPGWAGTPLARGVSEGLGL
                     AAYALNDVHAHALGEASFGAG"
     gene            complement(366..1283)
                     /locus_tag="PLB86_05490"
     CDS             complement(366..1283)
                     /locus_tag="PLB86_05490"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016663387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRC64621.1"
                     /translation="MPRGLTGVVPPLVTPLTPEGELDVPSLERLVARLLDAGVDGIFA
                     LGSSGEVAFFEDRMRAKVLDAVTAVVAGAVPVIAGVIDMQTRRVLEHVRAAEAAGVQA
                     VVATAPFYAITGPDEIRAHFRAIGAATDLPVYAYDLPICVHTKLAAPMLVELGLEGRI
                     VGVKDSSGDDVGFRWLCELNADAGSPLTLLTGHETVVDGAYLAGADGSVPGLANVDPA
                     GYVRLHRAAMAGDWATVRVEQSRLSRLMGIARAPIGKVGPAAGVGSFKTALALLGVLS
                     TNTMSLPMASLEGADVAAVRAVLEEGGLL"
     gene            complement(1283..2137)
                     /locus_tag="PLB86_05495"
     CDS             complement(1283..2137)
                     /locus_tag="PLB86_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC64622.1"
                     /translation="MSKQTTPQDQTVPQDHATAGAPVIELRGVHVIHKARTGKLFRPD
                     RVHAVKGVDFTVARGETVGIVGESGCGKSTVAKVMVGLQQPTEGQVLFHGKPIGTSAA
                     ARKELGRAVSVVFQDPATALNPRMTVRDQLLDPLGVHGIGTPATRLARVRELVHLVGL
                     PQSALEVLPRQISGGQRQRVAIARALALEPDVIVADEPTSALDVSVRAQVLNLLTDLK
                     EALGLGLVFISHDINTVRYVSDRIAVMYFGEIVETGPAEQIFTSPRNDYTRTLLSATP
                     SLLEIGAS"
     gene            complement(2134..4140)
                     /locus_tag="PLB86_05500"
     CDS             complement(2134..4140)
                     /locus_tag="PLB86_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HRC64623.1"
                     /translation="MLRPTLTKSLESRPGFRLPAIQSLPITSKIALGVIALVAAMAVF
                     APLLGVDPLASGTPVLPPGPGHPFGTDAIGRDILARVAFGARSSLVLGLVATGVALAA
                     AAVLGSIAATAGKATSEVLMRILDIVMSFPGIALAAVFVAVFGNALPVLIFAIAFLYT
                     PQLTRVVRANVLAQFGEDYVSASRVMGARPAHILIKHVARNCLAPIMVFATVLVADAI
                     VFEASLSFINAGVKPPNPSWGNILADGKQLLLSGYWWPTFFPGLMILITVLSLNILSE
                     GLTDALASPAVKRRVDVEADEKALLQGTAAAPVGAQVLGQVDGAVAKQLLTTSLVTLR
                     EQELKRTDRLVYAADSAPLLEVRDLSIRFPQAHGEVAIVDRVSFDVRPGETMALVGES
                     GCGKSITALAIMGLLSPAAQIEGEVFFDGRDLLTMDRKERIGLLGHDLAMIYQDALSS
                     LNPSMLVKSQLAQLTKRGGKRSAEDLLELVGLDPARTLKSYPHELSGGQRQRVLIAMA
                     LTRNPRLLIADEPTTALDVTVQQQVVGLLNTLREQLGFAMVFVSHDLALVAKLAHRIT
                     VMYAGQVVEQAPTSELLSNPRHEYTRGLLGSVLSIEAGAARLHQVPGTVPSPREFLPG
                     DRFAPRSSYPDLGRDERPTLRPAGPDHVYASTDALEAALEGSRR"
     gene            complement(4140..5105)
                     /locus_tag="PLB86_05505"
     CDS             complement(4140..5105)
                     /locus_tag="PLB86_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012577046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC64624.1"
                     /translation="MSNLLRLIGRRLVAFPLMVLGVTLLVFVVMSFSPSDPARLALGE
                     AASPDALAQYRTDHGLDDPLLVRYGSFLVGMLHGDLGTTSGNTPVTSVIAGAFPITLQ
                     LTLLGLVIAVVLALILGVVAALYRDRWPDQVIRVVSIMALATPSFWLAILLIQWLGTV
                     PGGWGMFPALVLGWVPFGEDPATYLNNVALPAFALGVPVAGSLTRVVRTAMVEELDRD
                     YVRTALGSGIPRREVIARNVLRNALITPITVLGLRVGYLMGGAVIIEIIFNIQAMGQL
                     ILDGVTRNDVYLVQGVTLTVAIAFIVVNILVDVLYVLVNPRIRSI"
     gene            complement(5244..6857)
                     /locus_tag="PLB86_05510"
     CDS             complement(5244..6857)
                     /locus_tag="PLB86_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC64625.1"
                     /translation="MQVLDQQSSRRGFLQLAGAMGAAAAFAGTVSACAPGSSTTTGAS
                     GAAGGAKADGTITAAISYELGTNGYDPMTTSSALTIAANWHVMEGLTEVDPASREVYA
                     ALAKELPKATGTSVDIALRDGAVFHNGSAVTADDVVFSIERVLDPANKSLYRQFIPFI
                     KGVAKKDDKTVTLTLAYPSALLAARLAVVKIVPKAVVTADAKAFDAKPIGTGPWKLTD
                     NGATSKQVTFERNDAYTGSKKARAKAMKWQIIPDASTRTNAVQSKSVQAIDSVPYLSI
                     DQLKTTSQVDSVQGFGLLFAMFNNSAGNPFATKENRQAFFYAIDMAKVVQTAMLGQAS
                     PATSFVQEGHPQYVKAKTVYSHDAAKATAMFKATGLTKLRLLCTDHDWVKKCTPLIQE
                     SLKAAGLEVEFTEKKSADVYNTIDGKPEAFDVVIAPGDPSVFGNDLDLLMRWWYAGDV
                     WTDTRMHWKGSAAYTQVQSLLDAGLQASDEAKAKESWASLIDLIADEIPLYPLFHRKT
                     PTAYDKASLVDFKPIPLTGLSFVGVASTK"
     gene            7004..7729
                     /locus_tag="PLB86_05515"
     CDS             7004..7729
                     /locus_tag="PLB86_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRC64626.1"
                     /translation="MSAPRKAPAWSTTDAIKQHIIDSGLGPGDLMPTETELCEALGVS
                     RSSVREAIRTLGSLDIVEVRHGHGTFVGRMSLDPLVNGMVFRLSMRGEHAVTALRNVV
                     QTRIALDLAMTDDLVKAYNGTYDSSMHALVDDMRTHTEQGHAFLDEDRDFHARLAKGV
                     DNQIIGELAGAFWEIHTRALPLLGISTPQDIMDTVEAHEAILLAVESGDVEAYRSAVQ
                     AHYLPLQRAIERAAHMIDDEPTP"
     gene            7731..9437
                     /locus_tag="PLB86_05520"
     CDS             7731..9437
                     /locus_tag="PLB86_05520"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRC64627.1"
                     /translation="MPRADVVIAESGVGYRQYRIPALALTPAGTCLAVFDARCDLDDL
                     PAPVDLVARRSTDGGRSFGPQTVVRAGTGVIGHGDASLVVDHESGSVFAFHATSAGVG
                     FFESSESIKDDDLGVLHCDVSVSHDDGVTWEHRRLSRDLRVGLNARLPEVGLGEAARV
                     SGLFAASGSGTQIRAGRFHGRLLQGFVALIGTQVYAAVAHSDDHGRTWVMGRPVGPGA
                     NENAVVWLGGDRVGLQSRAPGQRLMAWSDDGGDTFGPLEPAGDLLDPGNNGSLVRLDP
                     PGSVSTGDTGDTGDTGDGDDAGDPTGIRLACTHTSDPDLRRNLVVSTSTDDGATFTPA
                     VTLTAGAAGYSVAQPLPGGALGVLWEDEGYRRLIFTRLEPGWELPRAAHPALGPAPAL
                     VLDHVAAGHPATTSSPTPAWVAHPPIVASEWGPTIYKIVASRPGGPMLRSRAAYRELL
                     GDPAPGLHVNDILTFSARFPLPQVTDVRLDGVPVSPSHIVPVGSGTVLRGLRRVVRAQ
                     DVEAGVALVRVSATDGDRTAVLSARVPVELATASADPANPTRPAATAPAPAPVPVPLR
                     ST"
     gene            9434..10132
                     /locus_tag="PLB86_05525"
     CDS             9434..10132
                     /locus_tag="PLB86_05525"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HRC64628.1"
                     /translation="MNPLIAALRGTLVVSCQAYPGEPMRHPETMAQVAESAVRGGASA
                     IRAQGLADISHIAGRVDVPVIGLWKHGRGPVHITPTLRHARAVLDAGADIVAIDATAR
                     PRPDGLTVAQTVAGLRHSHPGAIIMGDCGSVEDGLAAADAGVDVVATTLSGYTGDRPR
                     TVGPDLELLAELVAALPHHAIVAEGRIHTPAQASAALAAGAHCVVVGTAITHPQSITG
                     WFAAALTSPVPQLP"
     gene            10195..10977
                     /gene="nagB"
                     /locus_tag="PLB86_05530"
     CDS             10195..10977
                     /gene="nagB"
                     /locus_tag="PLB86_05530"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRC64629.1"
                     /translation="MEVVITTPPQAASLVADAVTAIFRGRPDAVLGVATGSTPVLVYD
                     ELGRRVERGELSLARARAFMLDEYVGLPEGHPERYRTVIDRDFASKVDIDPANVHGPD
                     GLAEDLPAACAAYEAAIQAAGGVDLQLLGIGSDGHVAFNEPGSSLASRTRIKSLTRQT
                     REDNARFFDGDTEAVPRHCLTQGVATILAARHLVLMAFGKGKAEAVHHLAEGPVSAMW
                     PATALQLHPHVTVFVDDAAASRLQLGDYYRETFQAKPSWQGI"
     gene            complement(11038..12264)
                     /locus_tag="PLB86_05535"
     CDS             complement(11038..12264)
                     /locus_tag="PLB86_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRC64630.1"
                     /translation="MTYAARDRERWVAEDPAHKQSDRDDFARDRARVLHSAALRRLSA
                     KTQVVAPGSDDFIRTRLTHSLEVAQIGREFGSALGCDADVVDTACLSHDLGHPPFGHH
                     GESVLDELASDAGGFEGNAQTLRVLTRLEPKRVHDDGRPAGLNLTRASLDAVVKYPWA
                     RGQGPYETAKYGVYAADLPVFQWFREGADGGVRCLEAQVMDWSDDVAYSVHDVEDAIA
                     SGRVDPRVLYSPTEVRVVAELAQTAYAPDLSVEAFVAAAQRLVATGTVPHSFTGTRAD
                     LAALKDMTSRLIGRFLSAVEMATRSRYGPGSLARFAADLVIPDDVRAECSLLKAVAAH
                     YVMFTDERRSVMGRQEEVVRALVEGYRGHPERLDADLAADFAGAADDAARLRVVLDQI
                     ASLTDARAVTLARTHA"
     gene            complement(12261..>12449)
                     /locus_tag="PLB86_05540"
     CDS             complement(12261..>12449)
                     /locus_tag="PLB86_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3806 domain-containing protein"
                     /protein_id="HRC64631.1"
                     /translation="LARTTDLRWVTVEDPFGTDLGLFAESDYFALVPTNLVSGRFLNG
                     EIGWVPGVVGHLVELRNR"
     assembly_gap    12450..12549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12629..13309)
                     /locus_tag="PLB86_05545"
     CDS             complement(12629..13309)
                     /locus_tag="PLB86_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRC64632.1"
                     /translation="MTTQTEPPAGQPERPATFFESAAEFRDWLAANHETAPELWMGLR
                     KKHVEPRGLTWAEAVPEALCYGWIDSVVQSLGADAVRQRWTPRRNGSTWSKVNVALVA
                     QLEAEGRMQPAGRAAYERGRADRTGIYAYEQADSGAFPPEYAARLAASPAAPAFWERA
                     PASYRKQVIHWVLSAKQQATRDRRMDQLIESCAAYLPIPPLRYGDEAGWMRRARVELG
                     EPPAPPRP"
     gene            complement(13311..>14117)
                     /locus_tag="PLB86_05550"
     CDS             complement(13311..>14117)
                     /locus_tag="PLB86_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRC64633.1"
                     /translation="KGGGSALPWKRDLFRAIVAAVVAEGARRDIPVTVKMRIGIDDEH
                     LTYLDAGVAAEQEGAAAVALHARTAAQHYSGYAVWPAITALKQAVTTIPVLGNGDIWS
                     ADDAVRMVAETGCDGVVVGRGCLGRPWLFADLAAAFEGSTVRVQPTLGEVADTMRRHA
                     AYLVEFYDDENRGCRDIRKHIAWYLKGFPAGGTMRQHLALVDSLAALDRLLDGLDADA
                     PWPGADAEGQRGRAGTPKRVALPDGWLDSPDLSPEQQALVAQAELDVSGG"
     assembly_gap    14118..14217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14218..14360)
                     /locus_tag="PLB86_05555"
     CDS             complement(<14218..14360)
                     /locus_tag="PLB86_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRC64634.1"
                     /translation="MTAVAQSVLPVLPALPIGRHTVSTPVVLAPMAGITNRAFRRLCR
                     EHAP"
     gene            complement(14491..>16560)
                     /locus_tag="PLB86_05560"
     CDS             complement(14491..>16560)
                     /locus_tag="PLB86_05560"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRC64635.1"
                     /translation="VMLLSTGIVTTPQEQSLVGESARAQTLISQADFGDRPTERIIAT
                     VRTGSLTPEQAVALGAELAAAYQGVEGVARVGDPTPGADGRSVVIPLELAAARSATDT
                     TLPQPEEVVAPSLAVTTRLGAARPDLQVGQIGPGSITREFDATLGRDFQRAEFVSLPV
                     TLVILLIAFGAVVAAGVPLVLGIGSVAVALGLTAAVSNHLVTVDQNTQSMVLLIGLAV
                     GVDYALFVMRRAREERAAGASVEDSIVLAGATAGRAVIVSGLTVMVAMSGMLVAGGLF
                     TSLAIGTLLVVGVAVLASATVLPALLAVLGDRVDALRLPGTRRRQANAGSVESRWGRL
                     AGRVVARPVAWAATAAVALIALSVPALGMRTALTDVEGLPKDLAVVAAYRQLSAAAPT
                     KTVALLLTVKAPASAAPAVAEALAAAAPRAAALAHVSGVQPQASVSKDGTVTLLPIGV
                     ALDASDERLPGVVDAVRTQVAPQVTAALRGIPGVEVVVGGEAAATDLAQWMDSRLPWV
                     VGFVLILTLVVMLISFGSPWLAVATVGLNLLSVGAAYGVMTLVFQGTWAERLLDFTSI
                     GSIAAWLPLLMFVVLFGLSMDYHVFVTSRVREARDAGLDARAAVRLGVARSAGVVTSA
                     AAVMVGVFAVFGTLSVLELKQLGVGLAVAVLLDATVVRGVLLPAVLALLGDRAHQGPA
                     WLPRLHH"
BASE COUNT         2572 a         5732 c         5710 g         2346 t
ORIGIN      
        1 cccggccccg aacgaagcct cacccaacgc gtgcgcatgg acgtcgttga gcgcgtatgc
       61 cgcgagcccc aggccctcgc tcacgccgcg ggccagtggg gtccccgccc agcccggcag
      121 cgcatccgtg gcatgagtga caacaccatt cgtggagtcg atcacccccg ccgagccgat
      181 cccaaccagc accggacggc tctcgccgcg cgcttccttc gccacggcga ccgcagcggc
      241 gaccaccgcc tcaggcccct gcgaggccgg ggtggcgacc cggcatacct cgccgacgtg
      301 gccgcccgtg acgagagccg cggcgatctt cgtgccgccg atgtcgaggg cgagcacggg
      361 ctcgctcaca agagcccgcc ctcctccagg accgcacgca cggcggccac gtcggccccc
      421 tccaggctgg ccatgggcag cgacatggtg ttggtcgaca ggacgccgag caacgccagg
      481 gcggtcttga acgacccgac ccccgcggcc gggccgacct tgccgatcgg tgcgcgggcg
      541 atccccatga gtcgactcag ccgggactgc tcgacccgca ccgtcgccca gtcgcctgcc
      601 atcgccgctc gatgcagccg gacgtaaccg gccggatcga cattggccag accggggacg
      661 gatccatcgg cgccggcgag ataggcgccg tcgaccacgg tctcgtggcc ggtcagcagg
      721 gtgagcggtg agcccgcgtc ggcgttgagc tcacacagcc aacggaagcc gacgtcgtcg
      781 cccgaggagt ccttgacgcc gacgatccgc ccctccaggc cgagctcgac gagcatcggc
      841 gcagcgagct tggtgtggac gcagatcggc aggtcgtagg catagacggg caggtcggtg
      901 gccgccccaa tcgcccggaa gtgggccctg atctcgtcgg ggccggtgat ggcgtagaag
      961 ggcgcggtag cgaccacggc ctgcaccccg gccgcttcgg cggcccgcac gtgctccagg
     1021 acccggcggg tctgcatgtc gatgaccccg gcgatgacgg ggaccgcgcc ggccacgacg
     1081 gcggtcacgg cgtccaggac cttggcccgc atccgatcct cgaagaaggc cacttcgccg
     1141 gaggacccga gggcgaagat cccatcgacc ccggcgtcca ggagccgagc gaccaatcgc
     1201 tccagcgaag gcacatccag ctcaccctcg ggggtgagtg gagtgaccag gggcggcaca
     1261 acgccggtga ggccgcgggg catcacgaag ctccgatctc gagcaaggag ggggtagcgg
     1321 acaagagggt gcgggtgtag tcgttgcggg gcgacgtgaa gatctgctcg gccggaccgg
     1381 tctcgacgat ctccccgaaa tacatgacgg cgatccggtc agagacgtag cgcacggtgt
     1441 tgatgtcgtg cgagatgaag accaagccga ggccgagggc ttctttgagg tcggtgagca
     1501 ggttgagcac ctgcgcccgg accgacacgt ccaacgccga ggtcggctcg tcggccacga
     1561 tgacgtctgg ctcaagcgcc agggcccgag cgatcgcgac ccgttggcgc tgaccaccgg
     1621 agatctgccg gggcaggacc tccaaggccg actggggcag gccgacgagg tgcacaagct
     1681 cacggacccg agccaaccga gtcgcggggg tgccgatgcc gtgcacgccc aacggatcga
     1741 gcagttggtc gcgcacggtc atccgggggt tgagcgccgt cgccgggtcc tggaacacca
     1801 ccgacaccgc tcgaccaagc tccttgcggg ccgccgcgga cgtcccgatc ggcttgccgt
     1861 ggaagagcac ctggccctcg gtgggctgct gcagccccac catcaccttg gcgaccgtgg
     1921 acttcccaca gcccgactcc cccacgatgc cgacggtctc gccgcgggcc actgtgaagt
     1981 cgacaccctt gaccgcgtgg acgcggtcgg gccggaagag cttgccggtg cgcgccttgt
     2041 ggatgacgtg gacgccacga agctcgatga cgggcgctcc cgctgtggcg tgatcctgcg
     2101 ggaccgtctg atcctgcggg gtcgtctgct tgctcatcgg cggcttcctt ccagggcggc
     2161 ctcaagcgca tcggtcgagg cgtagacgtg gtcagggccg gccggacgca aggtcggccg
     2221 ctcgtccctg cccagatcgg ggtaggacga acgcggcgcg aaccggtcgc cgggcaggaa
     2281 ctcccgaggc gaggggaccg tgccgggcac ctgatgcagc cgcgcggctc ctgcctcgat
     2341 ggacaggacc gacccgagca gaccccgggt gtattcgtgc cgggggttgc tcaacaactc
     2401 gctcgtcggt gcctgctcga cgacctgacc ggcatacatg acggtgatcc ggtgggcgag
     2461 tttggcgacg agcgcgaggt cgtggctgac gaagaccatg gcgaaaccga gttgctcgcg
     2521 cagggtgttg agtagcccga cgacctgctg ctggacggtg acgtccagcg ccgtcgtcgg
     2581 ctcgtcggcg atgagcagcc gcgggttacg ggtgagcgcc atcgcgatga gcacccgctg
     2641 gcgctgaccg ccggagagct cgtgcggata ggacttcaac gtgcgggccg ggtcgaggcc
     2701 gaccaactcg agcaggtcct cggcgctgcg cttgccgccg cgcttggtca attgggccag
     2761 ttgcgacttg acgagcatcg acgggttcag cgagctgagc gcgtcctgat agatcatcgc
     2821 caggtcgtgg ccgagcaggc cgatgcgctc cttgcgatcc atggtcagca ggtcgcggcc
     2881 gtcgaagaag acctcgccct cgatctgcgc cgcgggcgag agcagaccca tgatcgccag
     2941 tgcggtgatc gacttgccac accccgactc gccgaccagc gccatcgtct cgccaggacg
     3001 cacgtcgaag gacacccgat cgacgatcgc gacctcccca tgggcctgcg ggaaacggat
     3061 cgacagatca cgcacctcga gcaacggcgc cgagtcggcg gcatacacca gtcggtcggt
     3121 gcgcttgagc tcctgctcgc gcaaggtgac cagactcgtt gtcagcagtt gcttggccac
     3181 cgcgccgtcg acctggccga ggacctgagc accgaccgga gcagcggcgg tgccctgcag
     3241 cagggccttc tcgtcggcct cgacgtcgac gcggcgcttg acggccgggc tggccagcgc
     3301 atcggtcaga ccctccgaga ggatgttgag gctcaacacg gtgatgagga tcatcaggcc
     3361 ggggaagaac gtcggccacc aatagcccga caggagtagc tgtttgccgt cggcgaggat
     3421 gttgccccag ctggggttcg ggggcttgac gcccgcgttg atgaacgaca gcgacgcctc
     3481 gaagacgatc gcgtcggcga cgagcaccgt cgcgaacacc atgatcggcg ccaggcagtt
     3541 gcgggcgacg tgcttgatga ggatgtgcgc cgggcgggca cccatgaccc gactcgccga
     3601 cacgtagtcc tcaccgaact gagcgagcac gttggcccgg acgactcggg tgagctgcgg
     3661 cgtgtaaagg aatgcgatcg cgaagatcag caccggcagg gcgttgccga acacggcgac
     3721 gaagacggcg gccagcgcga tcccggggaa gctcatgacg atgtcgagga tccgcatgag
     3781 gacctctgag gtcgccttgc cggccgtggc cgcgatcgag ccgagcaccg cagcggccgc
     3841 gagggcgaca ccggtggcca ccaggccgag gaccagcgac gagcgggcgc cgaaggccac
     3901 ccgggccagg atgtcgcggc cgatggcgtc ggtgccgaac gggtgacccg ggccgggagg
     3961 gagcaccggc gtcccgctgg ccagcgggtc gacaccgagc agcggcgcga acaccgccat
     4021 ggccgcgacg agggcgatga ctccgagggc gatcttggac gtgatcggca acgactggat
     4081 ggccggtagc cggaaaccag gccggctctc cagcgacttg gtcagggtcg ggcgcagcat
     4141 cagatgctcc tgatccgagg gttgaccagg acgtagagca cgtcgacgag gatgttgacc
     4201 acgatgaacg cgatcgcgac cgtcagcgtg acgccctgca cgaggtagac gtcgttgcgg
     4261 gtcaccccgt cgaggatgag ttggcccatc gcctggatgt tgaagatgat ttcgatgatg
     4321 accgcgccgc ccatgaggta gccgacccgc aggccgagca cggtgatcgg cgtgatgagc
     4381 gcgttgcgca ggacgttgcg ggcgatgacc tcacgccgtg ggatccccga cccgagagcg
     4441 gtgcgcacgt agtcgcggtc gagttcctcg accatggcgg tccgcacgac ccgggtgagg
     4501 gagccggcaa cgggcacacc gagggcgaac gccggcagcg ccacgttgtt gagataggtc
     4561 gccggatcct cgccgaacgg cacccagccc aacaccagtg cggggaacat cccccaccca
     4621 ccgggcacag tgcccagcca ttggatgagc aggatcgcca gccagaagga cggggtcgcc
     4681 agcgccatga tcgacacgac gcggatcacc tggtcgggcc agcggtcgcg atagagcgcc
     4741 gccaccacgc cgaggatcaa ggcgagcacg acggcgatga ccagcccgag cagggtgagt
     4801 tgcagcgtga tggggaaggc gccggcgatg acactcgtca ccggggtgtt tccgctggtc
     4861 gttcccagat ccccgtgcag catgccgacc aagaagctgc cgtagcggac gagcagcggg
     4921 tcgtccagcc catggtccgt gcggtactgc gccagggcat ccgggctggc cgcctctccc
     4981 agcgcgagcc gggcggggtc cgagggcgag aaggacatga cgacgaacac cagcaacgtc
     5041 acgccgagca ccatgagtgg gaaagcgacc agtctgcggc cgatgaggcg cagcaggttc
     5101 gacacaggag actcctcgtt cgcgtggggc ggtcaacggt ccgccacggg gatggtgcgc
     5161 aggggccggt atgccgcgca cgcggcatac cgggtccccg cacctagcaa gggggcacaa
     5221 cgggggcaca gtcccccgac ggcctacttc gtgctcgcca caccgacgaa cgacaggccg
     5281 gtgagcggga tcggcttgaa gtcgaccagg ctcgccttgt cgtaggcggt cggagtcttg
     5341 cggtggaaga gcgggtagag cgggatctcg tcggcgatga ggtcgatgag cgaggcccaa
     5401 ctctccttgg ccttggcctc gtcgctggcc tgcagcccgg cgtccaggag cgactggacc
     5461 tgggtgtatg ccgccgagcc cttccagtgc atccgggtgt cggtccacac gtcgccggcg
     5521 taccaccagc gcatgagcag gtcgaggtcg ttgccgaaca ccgaggggtc accgggagcg
     5581 atgacgacgt cgaaggcttc gggcttgccg tcgatggtgt tgtagacgtc ggcggacttc
     5641 ttctcggtga actccacctc gagcccggcg gccttgagcg actcctggat gagcggcgtg
     5701 cacttcttca cccagtcgtg gtcggtgcac agcagacgca acttggtcag gccggtcgcc
     5761 ttgaacatcg ccgtcgcctt cgcggcgtcg tggctgtaga cagtcttggc cttgacgtac
     5821 tgcgggtggc cttcctgcac gaagctcgtc gccggtgacg cctggccgag catggcggtc
     5881 tggacgacct tggccatgtc gatggcgtag aagaacgcct gccggttctc cttggtggcg
     5941 aaggggtttc ccgccgagtt gttgaacatc gcgaacaaca acccgaagcc ctgcaccgag
     6001 tcgacctgcg aggtggtctt gagctggtcg atcgagaggt aaggcaccga gtcgatcgcc
     6061 tggacgctct tggactggac cgcgttggtc cgcgtggagg cgtccgggat gatctgccac
     6121 ttcatcgcct tggcccgggc cttcttggag ccggtgtagg cgtcgttgcg ctcgaaggtg
     6181 acctgcttgc tcgtcgcgcc gttgtccgtg agcttccacg ggccagtgcc gattggcttg
     6241 gcgtcgaagg ccttggcgtc ggcggtgacg acggccttcg ggacgatctt gacgacggcc
     6301 agccgggccg ctagcagcgc cgacgggtag gccagcgtca gcgtgaccgt cttgtcgtcc
     6361 ttcttcgcca cgcccttgat gaaggggatg aactggcggt agagcgactt gttggccggg
     6421 tcgaggacac gctcgatgga gaacacgacg tcgtcggcgg tcacggccga gccgttgtgg
     6481 aacacggcgc cgtcccgcag cgcgatgtcc accgacgtcc cggtggcctt gggaagttcc
     6541 ttggcgaggg cggcatacac ctcgcggctg gcggggtcga cctcggtgag gccttccatg
     6601 acgtgccagt tggctgcgat ggtgagagcc gacgaggtgg tcatcgggtc gtacccgttg
     6661 gtgcccagct cgtaggagat cgcggcggtg atcgttccgt ccgccttcgc gccaccggct
     6721 gcgcccgacg cgcccgtcgt ggtggaggat cccggtgcgc acgcgctgac ggtgccggcg
     6781 aaggcggctg cggcacccat cgctccggca agctggagga accctcggcg gctggactgt
     6841 tggtcaagaa cctgcatggt gacggcgcct ttctgaggac cttacgtggg cggcagtgcc
     6901 cttcatccga catcggacgt ctgatgtccg atgatgatag tctgacgata ggcccggaag
     6961 tgccggggag tcaagaactg aagcagcgga ggacgacacg ccgatgagcg caccgcgcaa
     7021 ggcccctgcc tggtcgacga ccgatgcgat caagcagcac atcatcgact cgggtctcgg
     7081 cccaggtgac ctcatgccga ccgagaccga gctctgtgag gctctcggtg tctcgcgctc
     7141 gtcggtccgg gaggcgatcc gcacgctggg gtccctggac atcgtcgagg tgcgtcacgg
     7201 tcatggcacc ttcgtcgggc ggatgtcgct ggacccgctc gtcaacggga tggtgttccg
     7261 gctgtcgatg cgtggcgagc acgcggtgac tgccctgcgt aacgtcgtgc agacccggat
     7321 cgccctcgac ctggcgatga ccgacgatct cgtcaaggcc tacaacggca cctacgactc
     7381 gtcgatgcac gccctcgtgg acgacatgcg cactcacacc gagcagggcc acgccttcct
     7441 cgacgaagac cgcgacttcc atgcccgcct cgccaagggg gtggacaacc agatcatcgg
     7501 cgagctcgcc ggggccttct gggagatcca cacccgcgcc ctccccctgc tggggatcag
     7561 cacaccgcag gacatcatgg acaccgtcga ggcccacgaa gccatccttc tcgccgtcga
     7621 gtccggcgat gtcgaggcct accgcagcgc cgtccaggcc cactacctgc cactgcaacg
     7681 agcgatcgag cgggccgcgc acatgatcga cgacgaaccg accccctgag atgccgcgcg
     7741 ccgatgtcgt catcgcggag tccggagtgg gctaccgcca ataccgcatc cctgcgctcg
     7801 ccctcactcc cgccgggacc tgcctcgccg tcttcgatgc ccggtgcgac ctggacgacc
     7861 tgcccgcgcc cgtcgacctc gtcgcgcgcc gctccaccga cggcggtcgc tcgtttggcc
     7921 cacagaccgt cgtgcgggcg gggaccggcg tcatcggcca cggcgacgcg tccctcgttg
     7981 tcgaccacga gagcgggtcc gtcttcgcct tccacgccac ctccgcaggg gtcggcttct
     8041 tcgagtcgtc tgagtcgatc aaggacgacg acctcggggt gctgcactgc gacgtgtcgg
     8101 tctcccacga tgacggggtc acctgggagc atcgccggct cagtcgcgac cttcgcgtcg
     8161 gcctcaacgc tcgcctcccc gaggtcggcc tgggagaggc ggcccgggtg tcgggcctgt
     8221 tcgccgcctc cgggtccggg acccagatcc gcgccggacg ctttcacgga aggctgctgc
     8281 agggcttcgt cgcactcatc ggcacgcagg tgtatgccgc cgtcgcccac agcgacgacc
     8341 acggccgcac gtgggtcatg ggccgtcccg tggggccagg cgcgaacgag aacgccgtcg
     8401 tctggttggg tggcgaccgg gtcggcctgc agagccgggc acctggccaa cgcctcatgg
     8461 cctggtccga cgacggcggt gacacgttcg ggccgctgga gccggccggg gatctcctgg
     8521 acccggggaa caacggcagc ctcgtgcgcc tcgacccgcc gggcagcgtg tccacgggcg
     8581 acacgggcga cacgggcgac acgggcgacg gcgacgacgc tggcgatccc accggcatcc
     8641 ggttggcgtg cacccacacc agcgaccccg acctccggcg caacctcgtg gtgtccacct
     8701 ccaccgatga cggggccacc tttaccccgg cggtcaccct caccgcgggg gctgcgggat
     8761 actccgtcgc ccaaccactg cccgggggtg cccttggggt cctgtgggag gacgagggct
     8821 accgacgcct gatcttcacc cggcttgagc ccggctggga actcccccgc gccgcacacc
     8881 cagcgctagg cccggccccc gcgctcgtcc ttgaccacgt cgctgcgggc cacccggcca
     8941 ccacgtcgag cccgaccccc gcttgggtcg cccatccacc gatcgtggcg agcgagtggg
     9001 gtcccaccat ctacaagatc gtcgcctccc ggcccggcgg tccgatgctg cgcagccgcg
     9061 cggcataccg ggagctcctc ggtgacccgg caccagggct gcacgtgaac gacatcctca
     9121 ccttctcagc ccgcttcccg ttgccgcagg tcaccgacgt gcgcctggac ggcgtccccg
     9181 tctcacccag ccacatcgtg cccgtcggct ccggcacggt gctgcgcggg ctgcgccgcg
     9241 tcgtccgggc gcaggacgtc gaggccggcg tggcgctcgt gcgggtgtcg gccaccgacg
     9301 gtgaccgcac tgcggtcctg tcggcgcgcg tcccggtcga gctcgcgacc gcctcggccg
     9361 accccgccaa cccgactcgt cccgccgcta cggcacccgc tcccgcaccc gtcccggttc
     9421 ctttgaggtc cacgtgaatc cactgatcgc cgccctgcgc ggcacccttg tcgtctcctg
     9481 tcaggcctat cccggcgagc cgatgcgcca ccccgagacg atggcccaag tggccgagtc
     9541 agccgtccgt ggcggcgcct cagccatccg ggcccaaggc ctggcggaca tctcccacat
     9601 cgccggtcgg gtcgacgttc ccgtcatcgg cctgtggaag cacggccggg ggccggtcca
     9661 catcacgccg accctgcggc acgcgcgggc cgtcctcgac gccggcgccg acatcgtggc
     9721 gatcgacgca accgcccgcc cccgccccga cgggctcacg gtggcccaga cggtggccgg
     9781 gctgcgacac agccatcccg gcgcgatcat catgggtgac tgcgggtcgg tcgaggacgg
     9841 gctggccgct gccgacgccg gggtcgacgt ggtcgctacg acgctgagcg gctacaccgg
     9901 tgatcggccg cgtaccgtcg gccccgacct tgagctcctg gccgagctcg tcgccgccct
     9961 cccccatcac gcgatcgtcg ccgagggacg gatccacact ccggcgcagg cgagcgctgc
    10021 gctggctgcc ggcgcgcact gcgtcgtcgt cggaacggcc atcacccatc cccagtcgat
    10081 cactggctgg ttcgccgccg cgttgacctc acccgtgccg caactcccct gatctcgtcc
    10141 atcgttgatc tcaaccatcg ccaaaccgca acccagatag gaggcagacc tcagatggag
    10201 gttgtcatca ccaccccgcc ccaagcggcg tccctcgttg ccgacgctgt caccgcgatc
    10261 ttccgggggc ggcccgacgc cgtgctcggc gtcgcgaccg gctccacgcc ggtgctggtc
    10321 tacgacgagc tcggccgacg ggtcgagcgc ggcgagctca gcctcgcccg cgcccgggcg
    10381 ttcatgctcg atgaatacgt cgggttgcct gaaggacatc cggagcgcta ccgcacggtc
    10441 atcgaccgcg acttcgccag caaggtcgac atcgacccgg ccaacgtcca cggccccgat
    10501 ggtctggccg aggacctgcc ggcggcgtgc gcggcatacg aagcggcgat ccaggctgcc
    10561 ggcggagtgg accttcaact gctcgggatc ggctccgacg gtcacgtcgc cttcaacgaa
    10621 cccggctcct cgctggcctc gcggacccgc atcaagtcgc tgacccgcca gacgcgggag
    10681 gacaacgccc ggttcttcga cggtgacacc gaggcggtgc cgcggcattg cctcacccag
    10741 ggtgtggcga cgatcctcgc ggcccggcac ctggtgctca tggccttcgg gaagggcaag
    10801 gccgaggccg tccaccacct ggccgagggt ccggtgtcgg cgatgtggcc cgcgacggcg
    10861 ctgcaactgc atccgcatgt cacggtcttc gtcgacgacg ctgccgcctc tcgactgcaa
    10921 ctgggcgact actaccggga gaccttccag gccaagccga gttggcaggg gatctgacgg
    10981 gctgaggcct gcgccactgc ccgcgcgtgc gttcgtgcat gcgtccgcgc gggccgccta
    11041 ggcgtgcgtg cgcgccaggg tgacggcgcg cgcgtcggtg agtgacgcga tctggtcgag
    11101 gacgacccga agtcgggccg catcgtcggc ggcgcccgca aagtcggccg ccagatcggc
    11161 gtcgagccgc tccgggtgcc cgcgataacc ctccaccagg gcgcggacga cctcctcctg
    11221 gcgccccatg acggaccggc gctcatcggt gaacatcacg tagtgcgcgg ccacagcctt
    11281 gagcaacgag cactcggcgc ggacgtcgtc ggggatcact aggtcggccg cgaaccgggc
    11341 cagcgacccg gggccgtacc gcgatcgggt ggccatctcc acggcagaga ggaaccgtcc
    11401 gatcagccga ctggtcatgt ccttgagcgc cgcgagatcg gcgcgggtcc cggtgaagct
    11461 gtgcggcacc gttcccgtgg cgacgaggcg ctgggcggcc gccacgaacg cctcgaccga
    11521 cagatccggc gcgtatgccg tttgcgccag ttccgcaacc acccgcacct cggtgggtga
    11581 gtagagcacc cgcgggtcca cccgtccgga ggcgatggcg tcctcgacgt cgtgcaccga
    11641 ataggcgacg tcgtccgacc aatccatcac ctgggcctcc aggcaccgca ctcccccgtc
    11701 ggccccctcc cggaaccact ggaagaccgg caggtccgcg gcatacaccc cgtatttggc
    11761 tgtctcgtaa ggtccttggc cacgagccca gggatacttg accacggcgt cgaggctggc
    11821 ccgagtgagg ttgagcccag ccggccggcc gtcgtcgtgg acccgcttgg gctccagccg
    11881 ggtcaggacc cgcaacgtct gcgcgttgcc ctcgaacccc cctgcgtcac tagccaattc
    11941 atcgagcacc gactcgccgt ggtggccgaa gggcgggtgc ccgagatcgt gtgacaggca
    12001 ggccgtgtcg acgacatcgg catcacaccc cagcgccgac ccgaactccc gcccgatctg
    12061 ggcgacctcg agcgagtggg tgagccgcgt gcggatgaag tcgtccgagc cgggcgcgac
    12121 gacctgggtc ttggccgaca gccgccgcaa cgctgccgaa tgcaacaccc gggcccggtc
    12181 gcgggcgaag tcatcccggt cggactgctt gtgggcggga tcctcggcga cccaccgctc
    12241 gcggtcgcgt gcggcatacg tcatctgttg cgcaactcca cgaggtgtcc gacgaccccc
    12301 ggaacccagc cgatctcgcc gttgaggaag cgcccgctga cgaggttggt gggcaccaag
    12361 gcgaagtagt cgctctcggc gaacagcccg aggtcggtgc cgaacgggtc ctcgacggtg
    12421 acccaacgca gatcggtcgt ccgggcgagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnc ctcgccgacc ccgtcgagcg gggcgacgtc ctcggacggg tcatccttgc
    12601 gccgcgagaa cagtcccatg ccgccagcct agggccgggg cggcgcgggc ggttcgccca
    12661 gctcgacccg ggcccgccgc atccatccgg cttcgtcgcc atagcgcagc ggcgggatcg
    12721 gcaggtatgc cgcgcagctc tcgatcagct ggtccatccg ccggtctcgg gttgcctgtt
    12781 gtttggcgga gagcacccag tggatgacct gcttgcgata ggacgcgggt gctcgctccc
    12841 agaacgccgg ggcggccggg gaggcggcta ggcgcgcggc atactccggc gggaaggcgc
    12901 ccgagtcggc ctgctcgtag gcgtagatcc ccgtgcggtc ggcccgcccg cgctcgtatg
    12961 ccgcgcgtcc tgcgggctgc atgcggccct cggcctcgag ttgcgccacg agggcgacgt
    13021 tgaccttgct ccacgtcgag ccgttgcgac gcggcgtcca acgctggcgg acggcatcgg
    13081 cacccaggct ctgcacgacc gagtcgatcc agccgtagca cagggcttcg ggcaccgcct
    13141 cggcccaggt gagcccccgg ggctcgacgt gcttcttgcg cagacccatc cacaactcgg
    13201 gcgcggtctc gtggttggcg gccagccagt cgcggaactc cgccgccgac tcgaagaagg
    13261 ttgcgggccg ctccggctgg cccgctggcg gctctgtctg ggtggtcatc tcagccgccc
    13321 gagacgtcga gctcggcctg cgcgacgagg gcctgctgct cgggcgagag gtccggcgag
    13381 tccagccaac cgtcgggcag cgcgacccgt ttgggcgtcc cggcgcgccc gcgttgaccc
    13441 tcggcgtcgg cgccgggcca gggcgcgtcg gcgtcgagcc cgtccagcag ccggtcgagg
    13501 gcggccagcg agtcgacgag cgcgaggtgt tggcgcatcg tccctccggc agggaatccc
    13561 ttgagatacc aggcgatgtg cttgcggatg tcgcgacagc cgcggttctc gtcgtcgtag
    13621 aactccacga ggtatgccgc gtgccgccgc atcgtgtcag ccacttcgcc gagggtcggc
    13681 tgcactcgca cggtcgagcc ctcgaaggcc gccgcgagat cggcgaacag ccacggccga
    13741 ccgagacagc cccggccgac gacgactcca tcgcacccgg tctcggcgac catccggacg
    13801 gcgtcgtcgg ccgaccagat gtcaccgttg cccagcaccg ggatggtggt gaccgcctgc
    13861 ttgagggcgg tgatcgccgg ccacacggca tacccggagt agtgctgggc ggcggtgcgg
    13921 gcgtgcaacg cgacggcggc agcgccctcc tgctcggcgg cgacccccgc gtcgaggtag
    13981 gtgaggtgct catcgtcgat gccgatgcgc atcttgacgg tgaccgggat gtcgcggcga
    14041 gcgccctcag ccacgacagc ggcgacgatg gcgcggaaga ggtcgcgttt ccacggcagc
    14101 gcggagccgc cgcccttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggt
    14221 gcgtgctcgc ggcacaaccg ccggaaggcg cggttggtga tgcccgccat gggtgccagg
    14281 accacgggcg tcgacacggt atgccgcccg atcggcagcg cgggcagcac gggcagcacc
    14341 gactgcgcga ccgcggtcat cggggtctgc ggcgcggtga gggtggtctg ggcggtcacc
    14401 atggcggagc cattgtcgca acaccggctc agctgagcga aaccgacgtt ctgacgtcgg
    14461 tttcgctcag ctgaggtatc gccggccctg tcagtggtgc agccggggca gccaggcggg
    14521 gccttggtgc gctcggtcac ccagcagcgc gaggacggcc ggcagcagca caccccggac
    14581 gaccgtcgca tccagcagca cggcgacggc cagccccact ccgagttgct tgagctcgag
    14641 cacggacagc gtcccgaaca ccgcgaacac cccgaccatg acggctgcgg cgctcgtcac
    14701 gacgccggcc gacctggcca ccccgagtcg aacggcagcc cgcgcgtcga gcccggcatc
    14761 gcgggcttcc cgcacccggc tggtgacgaa gacgtggtag tccatcgaca gcccgaacag
    14821 cacgacgaac atcagcagcg gcagccacgc ggcaatcgac ccgatcgagg tgaagtccag
    14881 cagccgctcg gcccacgtcc cctggaagac gagcgtcatc acgccgtagg ccgcgcccac
    14941 cgaaaggagg ttgaggccga cggtggccac ggccaaccag ggcgagccga acgaaatgag
    15001 catgacgacc agcgtgagga tgagcacgaa gcccacgacc cacggaagtc ggctgtccat
    15061 ccattgggcc aggtcggtcg ctgccgcttc gcctccgacg accacctcga ccccaggtat
    15121 gccgcgcaag gccgccgtga cctgcggcgc cacctgggtg cggacggcgt cgacgacccc
    15181 gggcagacgt tcgtccgagg cgtcgagggc cacgccgatc ggcagcaagg tgacggtgcc
    15241 gtccttcgag acgctcgcct gcggctggac gcctgagacg tgcgcgagcg ccgccgctcg
    15301 gggagcagcc gcggccaagg cctctgccac cgcgggtgcg gcgctcgccg gggccttgac
    15361 ggtgagcagg agcgcgacgg tcttggtcgg tgctgctgcc gagagttgac ggtatgccgc
    15421 gacgacggcc aggtccttgg gcaggccctc gacgtcggtc aacgcggtcc gcatccccaa
    15481 ggcaggcacc gagagcgcga tgagcgcgac cgcagcggtg gcggcccagg ccacgggacg
    15541 agcgacgacg cggccggcga gcctccccca ccgcgactcg accgagccag cgttggcctg
    15601 gcgacgacgg gtgcccggca gtcgcagggc gtcgacccgg tcaccgagca cggccagcag
    15661 cgccggcagc accgtggcag aggcgaggac ggccactccg acaaccaaca acgtgccgat
    15721 ggccaacgag gtgaacagcc cgccagcgac gagcatcccc gacatggcca ccatgaccgt
    15781 gaggcccgag acgatgacgg cccgaccggc cgtcgctccg gcaaggacga tggagtcctc
    15841 gacgctcgcg ccggctgccc gttcctcgcg ggcccggcgc atgacgaaca gcgcgtagtc
    15901 gacaccgact gctagcccga tgagcaggac catgctctgg gtgttctggt ccacggtgac
    15961 caggtggttg gagaccgccg cggtgaggcc gagggcgacc gcgacgctgc cgatccccag
    16021 cacgagcggg acgccagcag cgacaaccgc accgaacgcg atgagcagga tcaccagggt
    16081 cacggggagg ctgacgaact cggcgcgctg gaagtccctg cccaacgtgg cgtcgaactc
    16141 gcgggtgatc gaccctggcc caatctgccc cacctgcagg tccggtcggg cggcacccag
    16201 gcgggtcgtc acggcgagcg acggggcgac gacctcctcg ggctgcggca gggtggtgtc
    16261 tgtggccgaa cgggccgcgg cgagttcgag ggggatgacg acgctgcgcc cgtctgcccc
    16321 cggcgtcggg tcacccacgc gggccacacc ctccacccct tgatatgccg ccgccagctc
    16381 ggctcccagc gccacggcct gctccggcgt gagagagccg gtgcggaccg tggcgatgat
    16441 ccgttcggtc gggcggtcgc cgaagtcggc ctgggagatg agggtctggg cccgtgccga
    16501 ctcgccgacg aggctctgct cctgcggggt ggtgacgatg ccggtcgaca gcagcatgac
//
LOCUS       DAORPW010000044        15914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_713_length_15914_cov_1054.759632, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000044 DAORPW010000000
VERSION     DAORPW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15914
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_713_length_15914_cov_1054.759632"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1959)
                     /gene="nuoL"
                     /locus_tag="PLB86_05565"
     CDS             complement(37..1959)
                     /gene="nuoL"
                     /locus_tag="PLB86_05565"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRC64636.1"
                     /translation="MPFAGEASTVHAATGFASAAWLLVALPLLGAAVLLLGGHRTDKW
                     GPLFATAMSWAAFVVGLGVFITLLGLSSEERALTVHLFNWVPAGQFQLEAGMLLDPLS
                     IAFVLLVTFVGSLIHVYSLGYMEHDPDKRRFFAYLNLFVAAMLLLVLANSYLLVYVGW
                     EGVGLASYLLIGFWNWNPPYASAANKAFVANRVGDVGMGLAIMAMFWQFGAVDFQTVN
                     GAVAEANEGALTVIGLFLLLAACGKSAQFPLQSWLGDAMAGPTPVSALIHAATMVTAG
                     VYLVVRSHAIFDGAPNAQLAVVIVGAITLVYGAIVGCAKDDIKKALAASTMSQIGYMM
                     LAAGLGPVGYAFAIFHLLTHGFFKAGMFLGAGSVMHGMNDKVDMRGFGNLSSVMKITW
                     VTFGLGWLAILGVPPFAGFWSKDKIIEAAFVGEGWRPWVFGLAALIGAGITAFYMSRL
                     FFMTFQGEKRWPKDAHPHESPRTMTIPMMVLAVGSASLGLILGPTNGILHWLEPVVGA
                     AEEEHPVIPVPALMAITLLVVAAGVALAWMRYWRDKVPTTAPVGSLLTRAARADLYQD
                     QVNEGVFMRPGIHLTRALVFLDKRGIDGAAGGFAALIGGTSSRLRRVQNGLARSYALT
                     MLIGVVVILGAVWVVQ"
     gene            complement(2013..2312)
                     /gene="nuoK"
                     /locus_tag="PLB86_05570"
     CDS             complement(2013..2312)
                     /gene="nuoK"
                     /locus_tag="PLB86_05570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRC64637.1"
                     /translation="MNLINYIYLSILLFTIGAATVLVRRNAIIIFMGIELMLNAANLA
                     FVTFSRIHGTLDGQVIALFVMVVAAAEVVVGLAIIMAIFRARRSASVDDANLLKL"
     gene            complement(2309..3070)
                     /locus_tag="PLB86_05575"
     CDS             complement(2309..3070)
                     /locus_tag="PLB86_05575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRC64638.1"
                     /translation="MSYAEAWLFWLIAPLMVLGALGLLFARKAVHAALCMAFVMVNLG
                     VLYLASQAEFLGIVQVFVYTGAVMMIFLFVLMLIGVDASDSLTETIRGHRAASLVVAL
                     ALAAVMFLAVGSFTLAEPAGLDKVNVDPGNVSGLADLIFGKYVWVFEITSALLITAAV
                     GAMVLAHRERLTPKSTQRELAARRVKDNVFVAGLPAPGVYARSNSVDTPALLPDGTPS
                     ELSVSRVLIAREQIVAPDGTSTAHASAEIEEGRSR"
     gene            complement(3067..3660)
                     /gene="nuoI"
                     /locus_tag="PLB86_05580"
     CDS             complement(3067..3660)
                     /gene="nuoI"
                     /locus_tag="PLB86_05580"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRC64639.1"
                     /translation="MGGFGVTWATMFRKVLTEEYPEEKRPTAPRYHGRHQLNRHPDGL
                     EKCIGCELCAWACPADAILVEGGNNDDTPADAGGTGRFSPGERFGRVYQINYLRCIFC
                     GLCIEACPTRALTMTNAYELADNNRADLIFTKEQLLAPLQQGMLAPPHAMVDGMEERD
                     YYLGKVNGSTEEQRVSAAAQAANAADTAMTSEGGATR"
     gene            complement(3776..5137)
                     /gene="nuoH"
                     /locus_tag="PLB86_05585"
     CDS             complement(3776..5137)
                     /gene="nuoH"
                     /locus_tag="PLB86_05585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRC64640.1"
                     /translation="MITSTLVPTIERPLADFSDTPLWLSLVKALFIFVYLLISTLLVI
                     WFERRIIGRMQQRPGPNRNGPFGLLQTLADGMKSMFKEDNAPAAADKFIFTLAPLIVA
                     TMCFVSFGIMPLGGDVVMWGHRTPLQLTDAPAAVLLVLAVASVGVYGIVLAGWSSGST
                     YPLLGGLRSSAQVISYEIAMGLSLVAVFIMSGSMSTSAIVDAQSSTWFVISAIPSFLV
                     YVVTMVGETNRLPFDLAEGEGEIVGGFHTEYGSMRFAMFFLAEYINMFTVSALATTMF
                     LGGWQAPPFIAAIGDGMLNAGWWGLLWFTIKVWSFMFFFVWLRGSLPRVRYDQFMRLG
                     WKLLIPLTLAWVVLVAFMRAAALGWFGTSTVISPFGRGLSAASLVLIGLVLVGLFAIG
                     FLLDRTSSSHAAAPVEQPDEIDPFAGGFPVPPLPGQTLREPERGIPAVAASTLTAKEE
                     SRG"
     gene            complement(5134..7575)
                     /locus_tag="PLB86_05590"
     CDS             complement(5134..7575)
                     /locus_tag="PLB86_05590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HRC64641.1"
                     /translation="MTVPTSPASAAGQAPPPDAVTLTIDGVTVSVPKGTLIIRAAEQA
                     GIEIPRFCDHPGLDPVGACRQCLVDVAAPRPDGTLAPFPKPQASCTMTVSPGMVVNTQ
                     HTSPVADKAQHGVMELLLINHPLDCPVCDKGGECPLQNQAMSNGRAQTRFDDVKRTFT
                     KPINISTEVLLDRERCIVCQRCTRFSDQVAGDPFINLVERGAMQQIGIYEEQPFESYF
                     SGNTVQICPVGALTGAAYRFRSRPFDLVSTPSTCEHCASGCAIRTDHRRGVVLRRMAI
                     DDPEVNGEWSCDKGRWAFHSATLGDRLELPLLRQADGSMRAVGWPEALEAAAAGLSAA
                     KGAGVLVGGRVSAEDAYAYGKFARVVLGTNDVDFRARPHSAAETAFLAEHVVATGPAG
                     GAVTYADLSEAKAVVLVGFEPEEESPIVFLRLRRAVRKARTAVYAVAPFASHGLEKLS
                     GTLLPTVPGAEAAALADPSLAAALAGGGIILVGERMATSPGALAAAVSLAASTGARLA
                     WIPRRAGERGALEAGALGALLPGGRPVTDAVARGEVAAVWEVDSLPSAPGRDTAGIVA
                     AAASGALGALVVGGVDPADLALPQVTAALAKAFVISLEVRESAVTAVADVVLPVAPHA
                     EKSGSFVNWEGRVRPFEAALSTNAMSDHRVLDMLAAELGFFMETRTHEQINDQFEALG
                     PWTGARAAANAAAAGSGATAYGSGDGDVVLATWPLLLDRGRMQDGEPFLAGTAHASVA
                     RVGATLAARLGVADGDAVTVSSGSGALSVPVAVTDGMVDGVVWLPTNSAGCAVRSTLG
                     VDAGARVTVSKGGAQ"
     gene            complement(7572..8888)
                     /gene="nuoF"
                     /locus_tag="PLB86_05595"
     CDS             complement(7572..8888)
                     /gene="nuoF"
                     /locus_tag="PLB86_05595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRC64642.1"
                     /translation="MTDTLTPVLTAHWDQQRSWTLAAYEQTGGYAALRKAMSMPAADV
                     LAVVKDSSIRGRGGAGFPAGMKWSFMSPPDGGPRYLVVNADESEPGTCKDIPLMMANP
                     HALIEGAAISQYAMGSSHAFIYLRGEVVHVYRRLLRAIEEATAAGYLGANVFGSGVTL
                     QVTAHAGAGAYICGEETALLDSLEGRRGQPRLKPPFPGAAGLYARPTSVNNVETIASV
                     PGILLNGASWFKSMGTEKSTGFGIFSLSGHVANPGQFEAPLGITMRQLIDLAGGIRKG
                     HQLKFWTPGGSSTPIFTEAHLDIPLDFDSVAAAGSMLGTRALQVFDETVSVVRAVARW
                     TDFYAHESCGKCTPCREGTWWMRQIMERLEHGQGLPGDIEKLDDICDNILGRSFCALG
                     DAATSPITSAIKHFRGEFEAGMHTPAAELFPPEKSVLFQVKEAAKA"
     gene            complement(8885..9667)
                     /gene="nuoE"
                     /locus_tag="PLB86_05600"
     CDS             complement(8885..9667)
                     /gene="nuoE"
                     /locus_tag="PLB86_05600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRC64643.1"
                     /translation="MSGHDASGHLHVSAESKAPYAADVLATLRQDAAAVIARYPVKRS
                     ALLPLLHLVQSVDGYVTGRGIELCAELLDLSTAEVSGVATFYTQYKRHPNGDYTLGVC
                     TNTLCAIMGGDAIWDAVSEHLGVGHDETTADGKVTLERIECNAACDYAPVVMTNWEFF
                     DNQTPDSVIRLADDLRAGKDVRPTRGPDKVCTFKEVSRVLAGFSDGRADEGPAAGPAS
                     LLGLKIAHEHGWAAPQPPAADPAPTAPAAPAAPAAPTKGASK"
     gene            complement(9664..11016)
                     /locus_tag="PLB86_05605"
     CDS             complement(9664..11016)
                     /locus_tag="PLB86_05605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRC64644.1"
                     /translation="MAQQSAEHTATGGAEEFLDSPAFTVSGSGDWDDLVDEATSLHEE
                     RIVVNMGPQHPSTHGVLRLILELDGETVTETRAGIGYLHTGIEKNMEYRTWVQGVTFC
                     TRMDYLTPMFQETAYCLAIEKLLGITDAIPERASVIRVLMMELTRISSHLVCIGTGGM
                     EMGATTVMTIGFRERERIFQIIEMITGLRMNNAYIRPGGVAQDLPPGAIDKIRELVPE
                     IRQGIRELELLVNENPIVKGRMVGVGHLDLAGCMALGITGPMLRSASLPHDLRKATPY
                     CGYETYDFDVITRDTADSYGRLRVRLDEMYQSLRIVLQCADRLEASVGDPVMVGDKKV
                     AWPAQLAIGTDGMGNSLDHIRTIMGESMEDLIHHFKLVTEGFRVPAGQVYVAVESAKG
                     ELGCHLVSDGGTRPYRAHFRDPSFNNLQATSIMCEGGQVADVIVAVASIDPVMGGVDR
                     "
     gene            complement(11040..11705)
                     /locus_tag="PLB86_05610"
     CDS             complement(11040..11705)
                     /locus_tag="PLB86_05610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRC64645.1"
                     /translation="MPVVIRERHGMFGATAGQDTTGYGGLTVPVVLPGGSERPYGGWF
                     DACVDDLARALDERNTASDEAIDRVVVDRGELTIVVAREHLPVVAQALRDDAALRFEL
                     CLGVSGVHYPGDTDNELHAVYHFQSITHGSKRIRVEVVCPDADPHIPSIVATYPATTW
                     HERETLDMFGIIFDGHPALTRILMPDDWPGHPQRKDYPLGGIPVEYKGATVPPADERR
                     AFN"
     gene            complement(11774..12328)
                     /locus_tag="PLB86_05615"
     CDS             complement(11774..12328)
                     /locus_tag="PLB86_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRC64646.1"
                     /translation="MGVEEKLPSGILLTTVELLAGYMRKASIWPATFGLACCAIEMMA
                     VGTPDYDIARFGMERFSATPRQADLMIVAGRVSQKMAPIVRQVYDQMPGPKWVISMGV
                     CASSGGMFNNYAIVQGVDHIVPVDIYLPGCPPRPEMLLDAIIKLHDKIQSAPLGANRI
                     AAEHAAESAALTAIPTSQMKGLLA"
     gene            complement(12342..12704)
                     /locus_tag="PLB86_05620"
     CDS             complement(12342..12704)
                     /locus_tag="PLB86_05620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRC64647.1"
                     /translation="MTESYVPLLFLLGLGGAFALVSVGTSLIVGPKRYNRAKMEAYEC
                     GIQPTPHASGGGRVPIKYYLTAMLFIIFDIESVFLYPFAVAFNQLGLFALVEMILFIV
                     TVFVAYAYVWRRGGLEWD"
     gene            complement(12798..14093)
                     /locus_tag="PLB86_05625"
     CDS             complement(12798..14093)
                     /locus_tag="PLB86_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HRC64648.1"
                     /translation="MTTGSADQAGDTADVIVVGAGPGGSATAAYLATQGVDVMLLEKA
                     TFPRDKICGDGLTPRAVRELVHLGVDTAGWQRTRGLRILGGGRNFTLDWPESATFPSY
                     GMVRTRKQFDHALAQHAVSRGARLHEGASVTAPLLDGRGRVTGVTAKVLDDAGRATGA
                     TATYRAKVVVAADGVSSRLATAVGRPKREDRVMGVAVRAYYTTPRRDDYLESHLELWT
                     TDDNGQRMLMPGYGWLFPLEDGRTNVGLGILDTSSAFGKTDYKDVLRRWADSLGDRWE
                     LAHDPAGGPIRGAALPMGFNRTPLYADGLLLVGDAAGMVNPFNGEGIDYALEAARVGA
                     DVIGQALARPSDAGRERVLAAYPRIMKAELGGYFTLGRWFAHAIGNPEVMRLATTYGL
                     PRTALMRFLLKVMANLPEEHGGRFDDRLVHLMSRIAPDA"
     gene            complement(14163..14870)
                     /locus_tag="PLB86_05630"
     CDS             complement(14163..14870)
                     /locus_tag="PLB86_05630"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HRC64649.1"
                     /translation="MTRADLDKAPQDVASMFDTVAERYDLTNDVLSAGQDRRWRKATV
                     AAVGAQPGQTVLDIAAGTGTSSEPFADAGVHVVPADFSLGMLRVGQRRRPDLAFTAAD
                     ATRLPFADASFDAVTMSFGLRNVVDVDGALREFARVTKPGGRLVVCEFSQPVWGPVRA
                     AYLNYLMRALPSIARRTSSNPESYVYLAESIRAWPDQRGLAARITDAGWADVQWRNLS
                     GGIVALHRATRPAPGAP"
     gene            14978..>15914
                     /locus_tag="PLB86_05635"
     CDS             14978..>15914
                     /locus_tag="PLB86_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRC64650.1"
                     /translation="MSTLRGPARAASDTVAPLVVRTVEIDDPGPLLALLPPAEQPGDV
                     LSWVRRGEGLVGWGQAATFTTAGPERFADADRWWRHTAARAVVRDEVGMPSSGLVCFG
                     SLPFADDSADRASFVVPRVSVGHKGNRWWVTTVGDVPTLAVTPAPTDDPLPPRNVAFA
                     DGALSSTAWAARVAEAVARINAGDLDKVVLARDLIVQADAPIDPRWVLRALADRYDNT
                     WVFSVSGMVGATPELLVRRERGLVTSRVLAGTIRRTGDDAKDLSLAASLARSSKDLEE
                     HEYAVRSVADALAPHCSSMNVPESPFVLHLPNVMHL"
BASE COUNT         2623 a         5271 c         5708 g         2312 t
ORIGIN      
        1 tcaggccgat gacggtcagc caggggaact ggttcatcac tgcaccaccc agacggcacc
       61 gaggatcacg acgacgccga tgagcatcgt cagggcatag gagcgggcca ggccgttctg
      121 gaccctacgc aaacgagagg acgtaccgcc gatgagggcc gcgaaacctc cggccgcccc
      181 atcgatcccg cgcttgtcta ggaagacgag cgcgcgggtg aggtggatgc cggggcgcat
      241 gaagacgccc tcgttgacct ggtcctggta gaggtccgct cgggccgcac gggtgagcag
      301 gctgcccacc ggtgccgttg tcgggacctt gtcacgccag tagcgcatcc aggccagcgc
      361 gacgccggcc gcgacgacga ggagcgtgat cgccatgagg gcggggaccg ggatcaccgg
      421 gtgctcctcc tcggcggccc cgacgaccgg ctcaagccag tgcaggatgc cgttggtcgg
      481 gccgaggatg aggccgagag aggccgaccc caccgccagc accatcatgg ggatggtcat
      541 cgtccgaggc gactcgtgcg ggtgagcatc cttgggccaa cgcttctcac cctggaaggt
      601 catgaagaac aaccgggaca tgtagaacgc ggtgatcccg gcgccgatga gggcagccag
      661 tccgaacacc cacggccgcc agccctcgcc gacgaaggcc gcctcgatga tcttgtcctt
      721 gctccagaag ccagcgaacg gcggcacacc gaggatggcc agccagccca agccgaaggt
      781 cacccaggtg atcttcatga cgctcgacag gttgccgaag ccccgcatgt cgaccttgtc
      841 gttcatgcca tgcatgaccg agccggcgcc gaggaacatc ccggccttga agaagccgtg
      901 ggtgagcagg tggaagatcg cgaacgcgta gccgaccggg ccgagaccgg ccgccagcat
      961 catgtagccg atctggctca tcgtcgaggc agccagggcc ttcttgatgt cgtccttggc
     1021 gcatccgacg atcgccccgt agacgagcgt gatcgcgccg acgatgacca cggccaactg
     1081 cgcgttgggt gcgccgtcga agatcgcgtg gctgcgcacc acgaggtaga caccggcggt
     1141 gaccatcgtc gcggcgtgga tgagtgccga caccggggtc ggaccggcca tcgcatcgcc
     1201 gagccagctc tgcaggggga actgcgccga cttgccgcag gcggccagca gcaggaagag
     1261 accgatgacg gtcaaggcgc cctcgttggc ctcggccaca gcaccgttga cggtctggaa
     1321 gtcgacggcc ccgaactgcc agaacatcgc catgatggcc aggcccatgc cgacgtcacc
     1381 gacgcggttg gcgacgaacg ccttgttggc ggcgctcgcg tagggcgggt tccagttcca
     1441 gaagccgatg agcaggtagg acgccagacc gacgccttcc cagccgacat agaccagcag
     1501 gtaggagttg gccagcacca gcaggagcat ggcggcgacg aagaggttga ggtaggcgaa
     1561 gaaccgtcgc ttgtcggggt cgtgctccat gtaacccagc gagtagacgt ggatgagcga
     1621 cccgacgaag gtcaccagca gcacgaaggc gatcgagagc gggtcgagca acatcccggc
     1681 ctcgagctgg aactgcccgg ccggcaccca gttgaacagg tgcaccgtca aggcccgctc
     1741 ttccgagctc aagcccagca acgtgatgaa cacgccgagc ccgacgacga acgcggccca
     1801 ggacatggcg gtcgcgaaca gcggccccca cttgtcggtg cggtggccgc cgagcagcag
     1861 gacggcagcg cccagcaggg gcagcgcgac gagcagccag gcggccgacg cgaagccagt
     1921 ggccgcgtgg accgtggacg cttcgcccgc gaacggcata cctgcgggca ggcctgccat
     1981 ggtggtcaga gagacgctca ctcgtttctc ccttacagct tcagcaggtt ggcgtcgtcg
     2041 accgaggccg atcggcgggc acggaagatg gccatgatga tcgccagacc gacgacgacc
     2101 tcagcagcgg ccacgaccat gacgaacagc gcgatgacct ggccgtcgag ggttccgtgg
     2161 atgcgtgaga aggtcacgaa ggcgaggttg gccgcgttga gcatcaactc gatgcccatg
     2221 aagatgatga tcgcgttgcg ccgcaccagg acggtggccg ccccgatcgt gaagagcagg
     2281 atcgacaggt agatgtagtt gatgaggttc accgcgacct cccctcctcg atctcggcac
     2341 tggcatgggc cgtcgaggtc ccgtcggggg cgacgatctg ctcacgcgcg atgaggactc
     2401 gcgagacgga caactcgctg ggtgtgccgt cgggcagcaa cgcgggggtg tcgaccgagt
     2461 tggaccgggc gtagacacca ggagccggca aaccggcgac gaacacgttg tccttgaccc
     2521 gccgagccgc caactcgcgc tgggtcgact tgggcgtgag tcgctcgcgg tgggccagca
     2581 ccatggcgcc cacggcagcc gtgatgagca gcgccgaggt gatctcgaag acccagacgt
     2641 acttgccgaa gatgaggtcg gccaacccac tgacgttgcc cgggtcgacg ttgaccttgt
     2701 cgaggccggc cggctcggcc agcgtgaagg accccaccgc aaggaacatg accgcggcga
     2761 gggccagagc caccaccagc gacgcggccc ggtgtccccg gatcgtctcg gtgagggagt
     2821 cagaggcgtc gacgccgatg agcatgagga cgaacaggaa gatcatcatc accgcgccgg
     2881 tgtagacgaa gacctggacg atgcccagga actccgcctg cgaggcgagg tagagcaccc
     2941 cgaggttgac catgacgaag gccatgcaca acgcggcgtg gaccgccttg cgggcgaaca
     3001 acagcccgag ggcccccagg accatgagcg gtgcaatgag ccagaacaac caggcttccg
     3061 cgtaactcat cgcgtggcac ctccttcgga ggtcatcgcg gtgtcggccg cgttagccgc
     3121 ctgggcagca gccgacacgc gctgctcctc cgtggacccg ttgaccttgc cgaggtagta
     3181 gtcgcgctct tccatgccgt cgaccatcgc gtgcggcggt gcgagcatgc cctgctggag
     3241 cggagcgagc agttgctcct tggtgaagat gaggtcggcc cggttgttgt cggccaactc
     3301 ataggcgttg gtcatcgtca gcgctcgcgt cgggcacgcc tcgatgcaca acccgcagaa
     3361 gatgcaccgc agatagttga tctggtagac gcggccgaag cgctcaccgg gtgagaaccg
     3421 accggtgccg cccgcgtcgg ccggcgtgtc gtcgttgttc ccgccttcga cgaggatcgc
     3481 gtcggccggg caggcccagg cacacagctc gcagccgatg cacttctcca gcccgtcggg
     3541 gtgccggttg agctggtggc gaccgtggta gcgaggcgcc gtggggcgct tctcctcggg
     3601 gtactcctcg gtgaggacct tgcggaacat ggtcgcccag gtgaccccga agccaccgat
     3661 cgacgccgga agcgcctcgc cgagccagcc cgcgcggggg gtgggctcgt cggagccggc
     3721 gtgcgtggtc gagggcaggt tggcgccgct ggtgtccggc ttcgccggca ggttgtcagc
     3781 cacggctctc ctccttcgcg gtgagggtcg aagcggcaac ggcaggtatg ccgcgctcgg
     3841 gctcgcgcag ggtctgcccc ggcagcggtg gcacagggaa accacccgcg aacgggtcga
     3901 tctcatcggg ttgctcgacc ggtgcggcgg cgtgcgacga ggatgtccga tcgagcagga
     3961 agccgatcgc gaacaacccg acgaggacca ggccgatgag caccagcgag gcggccgaga
     4021 gcccgcgacc gaaggggctg atcaccgtcg aggtgccgaa ccagccgagg gcggcggcgc
     4081 gcatgaacgc gacgagaacg acccacgcca gggtgagcgg gatgagcaac ttccagccca
     4141 gccgcatgaa ctggtcgtaa cgcacgcggg gcaacgagcc acgcagccag acgaagaaga
     4201 acatgaacga ccagaccttg atggtgaacc agagcaggcc ccaccagccg gcgttgagca
     4261 tgccgtcacc gatcgcggcg atgaacggcg gcgcttgcca gccgccgagg aacatcgtcg
     4321 tggcgagggc cgacaccgtg aacatgttga tgtactcggc gaggaagaac atcgcgaagc
     4381 gcatcgagcc gtattcggtg tggaagccac cgacgatctc gccttcgcct tcggccaggt
     4441 cgaagggcaa ccggttggtc tcgccgacca tcgtcaccac atagacgagg aacgacggga
     4501 tcgccgagat gacgaaccac gtgctgctct gggcatcgac gatggccgag gtcgacatcg
     4561 atccgctcat gatgaagacg gcgacgagcg acaggcccat ggcgatctca taggagatga
     4621 cctgggccga cgagcgcagc ccaccgagca gcgggtaggt cgagccggat gaccaaccgg
     4681 ccaggacgat gccgtagaca cccaccgacg cgacggcgag caccagcagc acggccgcgg
     4741 gagcgtcggt caactgcagc ggggtgcggt gcccccacat gacgacgtcc ccacccaacg
     4801 gcatgatccc gaacgagacg aaacacatgg tcgcgacgat cagcggtgcc aaggtgaaga
     4861 tgaacttgtc ggcggccgcc ggcgcgttgt cttccttgaa catcgacttc ataccgtcgg
     4921 cgagcgtctg caacagaccg aacgggccgt tgcggttggg gcccggacgc tgctgcatcc
     4981 ggccgatgat gcgccgctcg aaccagatga ccaacagtgt cgagatgagc aggtagacga
     5041 agatgaacag cgccttgacc agcgacagcc acagcggcgt gtcgctgaaa tcggcgagag
     5101 gccgttcgat cgtcgggacg agggtcgacg tgatcactgg gcacctccct tcgacaccgt
     5161 gactcgagcg ccggcatcga cgccgagggt ggagcggacc gcgcagcccg cggagttggt
     5221 cggcagccag accacgccgt cgaccatgcc gtcggtcacc gcgacgggca cgctgagcgc
     5281 gccactgccg ctggagaccg tgaccgcgtc accgtcggcg acaccgaggc gcgccgcgag
     5341 ggtggcaccg acccgagcca cgctcgcatg cgcggtgcca gccaggaacg gctcgccgtc
     5401 ctgcatccgg ccgcggtcga gcaacagcgg ccaggtcgcc agaacgacgt caccgtcgcc
     5461 cgatccgtat gccgtcgcgc cgctaccggc cgctgcggcg ttcgccgcgg cccgggcacc
     5521 ggtccacgga ccgagggcct cgaactggtc gttgatctgc tcgtgggtgc gggtctccat
     5581 gaagaagccc aactcggcgg cgagcatgtc caacacgcgg tgatcgctca tcgcgttggt
     5641 cgacagcgct gcctcgaagg gccggacccg gccctcccag ttgacgaaac tgccggactt
     5701 ctcggcgtgc ggggccaccg gcagcaccac gtcggcgacg gcggtcactg cggactcgcg
     5761 cacctcgagg ctgatgacga aggccttggc caacgcggcg gtgacctgcg gcagcgcgag
     5821 gtcggccggg tcgacaccac cgacaaccaa cgctccgagg gcaccgctgg cggcggcagc
     5881 aacgatccca gcggtgtcgc gaccaggggc actcggcagg gagtcgacct cccacaccgc
     5941 ggcaacctca ccgcgagcaa cggcgtcggt caccgggcga ccgccaggaa gcagggcgcc
     6001 gagcgcaccg gcctcaagag caccgcgctc accggcgcga cgcgggatcc aggccagtcg
     6061 ggctccggtc gaggcggcca gggagaccgc agcggccagc gcgccgggcg aggtggccat
     6121 ccgctcgccg acgaggatga tgcccccgcc cgcgagagcc gcagccaaac tcgggtcggc
     6181 cagcgcggcc gcctcggctc cggggacggt cggcaggagg gtcccggaga gcttctccag
     6241 accgtgcgag gcgaaggggg ccacggcata cacggccgtc ctggccttgc ggaccgcgcg
     6301 ccgcagccgc aggaagacga tcggcgactc ctcttcgggc tcgaacccga cgaggacgac
     6361 ggccttggcc tccgagaggt cggcgtaggt caccgcgcca ccggcggggc cggtcgcgac
     6421 gacgtgctcg gccaggaagg ccgtctcggc cgcagagtgc ggacgcgccc ggaagtcgac
     6481 gtcgttggtg cccaagacca cgcgggcgaa cttgccgtag gcgtaggcgt cctcggcgga
     6541 cacccggccc ccgacgagca ccccggcacc cttggcggcg ctcaggccgg cagcggccgc
     6601 ttcgagggcc tcgggccagc cgaccgcacg catcgagccg tcggcctggc gcagcaacgg
     6661 caactccagc cggtcgccga gcgtcgccga gtggaacgcc cagcggccct tgtcacagga
     6721 ccactcgccg ttgacctcgg ggtcgtcgat cgccatccgg cgcagcacga cgccgcgacg
     6781 gtggtcggtg cggatcgcac agcccgacgc gcagtgctcg caggtgctcg gggtcgacac
     6841 gaggtcgaag ggccgcgagc ggaaccggta tgccgcgcct gtcaaggccc cgacggggca
     6901 gatctggacg gtgttaccgg agaagtagga ctcgaacggc tgctcttcgt agatgccgat
     6961 ctgctgcatc gccccgcgct cgacgaggtt gatgaacggg tcaccggcga cctggtcgga
     7021 gaagcgggtg cagcgctgac acacgatgca gcgctcgcgg tcgagcaaca cctcggtgga
     7081 gatgttgatc ggcttggtga aggtgcgctt gacgtcgtcg aaccgagtct gggcgcggcc
     7141 gttggacatc gcctggttct gcagggggca ctcgccgccc ttgtcgcaga cggggcagtc
     7201 cagcgggtgg ttgatgagca gcaactccat gacaccgtgc tgagccttgt cggcgaccgg
     7261 cgaggtgtgc tgggtgttga cgaccatgcc ggggctgacc gtcatggtgc acgaggcctg
     7321 cggcttgggg aacggcgcca gggtgccgtc ggggcgcggg gccgcgacgt cgacgaggca
     7381 ctggcggcag gcgcccaccg ggtcgaggcc ggggtggtcg cagaaccgag ggatctcgat
     7441 gccggcctgt tcggcggcgc ggatgatgag cgtgcccttg ggcacgctca cggtcacgcc
     7501 gtcgatcgtc agggtcaccg cgtcgggcgg cggcgcctgc ccggcggcag aagcggggct
     7561 ggtcgggacg gtcatgcctt cgcggcctcc ttgacctgga acaggaccga cttctccggc
     7621 gggaacagct cggcggccgg ggtgtgcatg ccggcctcga actctccccg gaagtgcttg
     7681 atcgcggagg tgatcgggct ggtggccgcg tcgccgagcg cgcagaacga gcggcccaag
     7741 atgttgtcgc agatgtcgtc gagcttctcg atgtcgccgg gcagaccctg gccatgctcg
     7801 aggcgctcca tgatctggcg catccaccac gtgccctcac ggcagggcgt gcacttgccg
     7861 caggactcgt gggcgtagaa gtcggtccag cgggcgactg cccggacgac cgagacggtc
     7921 tcgtcgaaca cctgcagcgc ccgcgtgccg agcatcgacc cggcggcggc caccgagtcg
     7981 aagtcgagcg ggatgtccag gtgagcttcg gtgaagatcg gggtcgagga cccacccggc
     8041 gtccagaact tcagctggtg acccttgcgg atcccgccgg cgaggtcgat gagctggcgc
     8101 atggtgatgc ccagcggcgc ctcgaactgg ccggggttgg cgacgtgccc ggagaggctg
     8161 aagatgccga agccggtcga cttctcggtg cccatcgact tgaaccagct cgcgccgttg
     8221 agcaggatgc ccggcaccga ggcgatggtc tcgacgttgt tgaccgaggt cgggcgggcg
     8281 tagaggcccg ccgcgccggg gaacgggggc ttgagccgcg gttggccgcg gcgaccctca
     8341 agggagtcca gcagggccgt ctcttcgcca cagatgtagg cgccggcacc ggcgtgggcc
     8401 gtgacctgaa gggtgacgcc cgagccaaag acgttggccc cgaggtaacc cgctgcggtc
     8461 gcctcctcga tggcccgcag gagccgccgg tagacgtgga cgacctcgcc gcgcagatag
     8521 atgaacgcgt ggctcgaccc catcgcatac tggctgatcg ccgcgccctc gatgagggcg
     8581 tgcgggttgg ccatcatgag cgggatgtcc ttgcaggtgc ccggctcgga ctcgtcagcg
     8641 ttgacgacga ggtagcgcgg accgccgtcg ggcggggaca tgaacgacca cttcatgccg
     8701 gccgggaagc cggcgccacc gcggccgcgg atcgaggagt ccttgacgac ggcgagcacg
     8761 tcggcggccg gcatgctcat ggccttgcgg agggcggcat acccacccgt ttgctcgtat
     8821 gccgcgagcg tccaggaacg ctgctggtcc cagtgggccg tgaggaccgg ggtgagggtg
     8881 tcggtcactt cgaggccccc ttggtcggtg cagccggtgc agccggtgca gccggtgcgg
     8941 tcggtgccgg gtcggcggcc ggcggctgcg gcgcagccca tccgtgttcg tgggcgatct
     9001 tcaggccgag caaagacgcg ggcccggcgg ccgggccctc gtctgcgcgg ccatcgctga
     9061 agcccgccag cacgcgggac acctctttga aggtgcacac cttgtcggga ccgcgggtgg
     9121 ggcggacgtc cttgccggcc cgcaggtcgt cggcgaggcg gatcaccgag tcgggcgtct
     9181 ggttgtcgaa gaactcccag ttggtcatga cgaccggggc gtagtcgcac gccgcgttgc
     9241 actcgatgcg ctcaagggtg accttgccgt cggccgtggt ctcgtcgtgg cccaccccga
     9301 ggtgctcgga cacggcgtcc cagatggcgt cgccgcccat gatcgcgcag agcgtgttgg
     9361 tgcagacgcc gagcgtgtag tcgccgttgg ggtggcgctt gtattgcgtg tagaaggtcg
     9421 cgaccccgct cacctcggcg gtgctcaggt cgagcaactc ggcgcagagc tcgatgcctc
     9481 ggccggtgac gtagccgtcg accgactgca cgaggtgcag cagcggcagc agcgccgaac
     9541 gcttgaccgg gtatcgggcg atcaccgcgg cggcgtcctg ccggagggtg gccaggacgt
     9601 ccgcggcata cggcgccttg ctctccgcgc tgacgtgcag gtggcctgat gcgtcgtggc
     9661 cgctcaccgg tccacacctc ccatgacggg gtcgatcgat gcgacggcga cgatgacgtc
     9721 agcgacctgg ccgccttcgc acatgatcga cgtcgcttgc aggttgttga acgagggatc
     9781 gcggaagtgc gcgcgatagg ggcgggtgcc gccgtcggag accaggtggc agccgagctc
     9841 gcccttggcg ctctcgaccg cgacgtagac ctgaccggcc gggacccgga agccctcggt
     9901 gacgagcttg aagtgatgga tgaggtcttc catcgactcg cccatgatcg tgcggatgtg
     9961 gtcgaggctg ttgcccatgc cgtcggtgcc gatggccaac tgggcgggcc aggcgacctt
    10021 cttgtcaccg accatgaccg ggtcacccac gctggcctca agcctgtcgg cgcactgcag
    10081 gacgatccgc aacgactggt acatctcgtc gaggcggacc cgcagccggc cgtaggagtc
    10141 ggcggtgtcg cgagtgatga cgtcgaagtc gtaggtctcg taaccgcaat agggggtcgc
    10201 cttgcgcagg tcgtgcggca ggctcgccga gcggagcatc gggccggtga tgccgagggc
    10261 catgcagccg gccaggtcga ggtgaccgac gccgaccatg cggcccttga cgatggggtt
    10321 ctcgttgacg agcaactcca actcgcggat gccctggcgg atctcgggca ccagctcgcg
    10381 gatcttgtcg atcgcgcccg gaggcaggtc ctgggcgaca ccaccggggc ggatgtaggc
    10441 gttgttcatc cgcaggccgg tgatcatctc gatgatctgg aagatccgct cgcgctcacg
    10501 gaaaccgatc gtcatgaccg tggtggcgcc catctccatg ccaccggtgc cgatacacac
    10561 caggtgcgag gagatccggg tgagctccat catgaggacc cggatgactg aggcccgctc
    10621 agggatggcg tcggtgatgc cgaggagctt ctcgatggcc aggcagtagg ccgtctcctg
    10681 gaacatcggg gtgaggtagt ccatccgggt gcagaaggtc accccctgga cccacgtgcg
    10741 gtattccatg ttcttctcga taccggtgtg caagtagccg attcccgcgc gggtctcggt
    10801 caccgtctcg ccgtcgagct cgaggatgag ccgcaggacg ccgtgggtcg acgggtgctg
    10861 cggacccatg ttgacgacga tccgctcctc atgcagcgag gtggcctcgt cgacgaggtc
    10921 gtcccagtcg ccggagccgc tcacggtgaa ggccggagag tcgaggaact cctcggcgcc
    10981 gccggtcgcg gtgtgctctg cactctgttg tgccatgtca ggtcccaggt cggcggccat
    11041 cagttgaacg ccctccgctc gtcggccggg ggcacggtcg cgcccttgta ttcgaccggg
    11101 atgccaccca gcgggtagtc cttgcgctgc gggtggccgg gccagtcgtc gggcatgagg
    11161 atccgcgtca gggccgggtg gccgtcgaag atgatcccga acatgtccaa ggtctcgcgt
    11221 tcgtgccagg tcgtcgcggg gtaggtcgcg acgatcgacg ggatgtgcgg atcggcgtcg
    11281 gggcagacga cctcgacgcg aatccgcttg ctgccgtggg tgatcgactg gaagtgatag
    11341 acggcgtgca actcgttgtc ggtgtcgccg gggtagtgga cccccgacac cccgagacac
    11401 aactcgaagc ggagcgcagc gtcgtcgcgc agtgcctgcg ccacgaccgg caggtgctcg
    11461 cgggcgacga caatggtcaa ctcgccgcgg tcgacgacga cccgatcgat cgcctcgtcg
    11521 gacgccgtgt tgcgctcgtc gagcgcccga gccaggtcgt cgacgcacgc gtcgaaccag
    11581 ccgccgtagg ggcgctccga cccgcccggc aggacgaccg gaacggtcag gccgccgtaa
    11641 ccggtggtgt cttggccggc ggtggcaccg aacatcccgt gccgctcgcg gatgacgacc
    11701 ggcataccga gagcgacggt gcgagcaccg gaagtggccc cagcggcgac ctcctggccc
    11761 aggtcatcgg cgctcatgcc agcagcccct tcatctggga ggtcgggatc gcagtgagcg
    11821 cggcgctctc ggcggcgtgc tcagcggcaa tccggttggc gcccaacggc gccgactgga
    11881 tcttgtcgtg cagcttgatg atggcgtcca gcagcatctc ggggcgcggt ggacagcccg
    11941 gcaagtagat gtcgaccggg acgatgtggt cgacgccctg gacgatcgcg tagttgttga
    12001 acatgccgcc cgagctcgcg cagacgccca tggagatgac ccacttgggg ccgggcatct
    12061 ggtcgtagac ctggcggacg atgggggcca tcttctggct cacccggccg gcgacgatca
    12121 tcaggtcggc ctggcgcggg gtcgcggaga agcgctccat gccgaaccgc gcgatgtcgt
    12181 agtcgggcgt gccgacggcc atcatctcga tggcgcaaca ggccagcccg aacgtcgcag
    12241 gccagatcga ggccttgcgc atgtagccgg ccagcagctc gacggtggtg agcagaatcc
    12301 ccgaggggag cttctcttcc acacccattc cgatgcctcc ctcagtccca ctcgaggccg
    12361 ccacgacgcc agacataggc gtaggcgacg aagacggtca cgatgaacag gatcatctcg
    12421 acgagcgcga acaggcccag ttggttgaag gccaccgcga acgggtacag gaacaccgat
    12481 tcgatgtcga agatgatgaa cagcatggcg gtcaagtagt acttgatcgg cactcggccg
    12541 ccgccggacg cgtgtggagt gggctggatg ccgcactcat acgcctccat cttggcccgg
    12601 ttgtagcgct tggggccaac gatgagcgag gtgcccacgg agaccagcgc gaacgctcct
    12661 ccgagcccca gcaggaacaa cagcggaacg taggactcgg tcatccgtcg ctccctttca
    12721 gggtgggtgc atgcaggccg tcccccatcc taggggggcc tggaactgcg tcgttcatgc
    12781 cgttggtccc cgtcatctca ggcgtccggg gctatgcgcg acatcaggtg gacgaggcgg
    12841 tcgtcgaagc gaccaccatg ctcctcgggg aggttggcca tcaccttgag gaggaagcgc
    12901 atgagcgccg tgcgcggcag gccgtaggtc gtcgccaacc gcatcacctc ggggttgccg
    12961 atggcgtggg cgaaccaccg acccagcgtg aagtagccac ccagctcggc cttcatgatc
    13021 cgcgggtatg ccgcgagcac ccgttcgcgt ccggcgtcac tcgggcgggc gagggcttgc
    13081 ccgatgacgt cggcaccgac ccgagccgct tcgagggcgt agtcgatgcc ttcgccgttg
    13141 aacgggttga ccatgccggc ggcgtcgccg acgagaagga ggccatcggc atacaggggg
    13201 gtgcggttga agcccatcgg cagggcggcg ccgcggatgg ggccaccggc cgggtcgtgc
    13261 gcgagctccc accggtcacc gaggctgtcg gcccagcgcc gcaggacgtc cttgtagtcg
    13321 gtcttgccga acgcgctgct cgtgtcgagg atgcccaggc cgacgttggt gcgaccgtct
    13381 tccagcggga agagccagcc gtagcccggc atgagcatcc gctgaccgtt gtcgtcggtg
    13441 gtccacaact cgaggtgcga ctcgaggtag tcgtcgcgcc ggggggtcgt gtagtaggcg
    13501 cggaccgcca cgcccatgac gcggtcctcg cgcttggggc ggccgaccgc cgtggccagg
    13561 cgcgaggaga ccccgtccgc ggcgacgacg actttggcgc ggtaggtcgc ggtcgctccg
    13621 gtggcgcgac ccgcgtcgtc gagcaccttg gcggtgacgc cggtgacccg gccacgacca
    13681 tcgagcaggg gcgcggtgac gctggcgcct tcatgcagtc gggcgccccg agagacggcg
    13741 tgttgcgcga gcgcgtggtc gaactgcttg cgggtgcgga ccatgccgta ggacgggaag
    13801 gtcgcgctct cgggccagtc gagggtgaag ttgcggccgc cgccgaggat gcgcaggccg
    13861 cgggtgcgtt gccacccggc cgtgtcgacg ccgaggtgga ccaactcgcg cacggcgcgg
    13921 ggcgtgaggc cgtcaccgca gatcttgtcc cgtgggaacg tcgccttctc caggagcatg
    13981 acgtcgacgc cctgggtagc caggtaggcc gccgtggccg agcctcctgg cccggctccg
    14041 acgacgatga cgtcggcggt gtcgcccgcc tggtcagccg atccggtggt cactctcgcc
    14101 ctcggtgcgg tggggcgccg gtgaaggcgc cgcttgtgaa gattggcacg aacccgccca
    14161 gtctaggggg caccgggggc ggggcgggtc gcgcggtgga gagcgacgat gcctccactg
    14221 aggttgcgcc actgcacgtc ggcccagccg gcgtcggtga tccgcgccgc gagcccccgc
    14281 tggtcgggcc aggcgcggat cgactcggcg aggtagacgt aggactccgg gttggaggac
    14341 gtccgccgcg cgatcgaggg cagcgcgcgc atcagatagt tgaggtatgc cgcccgcacc
    14401 ggaccccaca ccggctggct gaactcgcac acgacgaggc gacccccggg cttggtcacc
    14461 cgggcgaact cccgcagcgc cccgtcgaca tcgacgacgt tgcgcagccc gaacgacatg
    14521 gtcaccgcgt cgaaggacgc gtcagcgaag ggcaaccggg tcgcgtcggc cgcagtgaag
    14581 gccaggtcgg ggcgccgtcg ctggccgacc cgcagcatcc ccagtgagaa gtcggccggc
    14641 acgacgtgga cccctgcgtc ggcgaacggc tcggagctgg tcccggtgcc ggcggcgata
    14701 tcgagcaccg tctggccggg ctgggcgccg acggccgcga ccgtggcctt gcgccaacgc
    14761 cggtcctgtc ccgccgacaa gacgtcgttg gtgaggtcgt aacgctcggc caccgtgtcg
    14821 aacatcgacg cgacgtcctg gggtgccttg tccagatcgg cccgggtcat ggctggcatc
    14881 cttccaccct ccgcgacccc tcccctgcac ccgcactctg gcgggcggcg cgtgggcggc
    14941 gagcgggcgg cataccgagt cgtaacattg accttcgatg tccaccctcc gcgggcccgc
    15001 ccgggccgcc tccgacaccg tcgcgccgct cgtcgtgcgc acggtggaga tcgacgaccc
    15061 cgggccgctg ctggccctcc tccctcccgc cgagcaaccg ggcgacgtgt tgtcgtgggt
    15121 ccggcgcggc gagggcctcg tcgggtgggg gcaggccgcc acgttcacga cggccggacc
    15181 cgagcgcttc gcggacgcag accggtggtg gcggcatacc gctgctcgcg ccgtggtccg
    15241 cgacgaggtg ggtatgccgt ccagcgggct cgtctgcttc gggagcctgc cgttcgcgga
    15301 cgactccgcc gaccgcgcca gcttcgtcgt cccgcgggtc agcgtcggcc acaagggcaa
    15361 ccggtggtgg gtgaccacgg tcggcgacgt cccgacgctt gccgtcacgc cggccccgac
    15421 cgacgacccg ctgccgcccc gcaacgtcgc cttcgccgac ggtgccctgt cgagcaccgc
    15481 gtgggctgcc cgcgtggccg aggcggtcgc gcggatcaac gcgggcgacc tcgacaaggt
    15541 cgtcctcgcc cgtgacctca tcgtgcaggc cgacgcgccg atcgacccgc ggtgggtgct
    15601 gcgggcgttg gccgatcgct acgacaacac gtgggtgttc tcggtctccg gcatggtcgg
    15661 tgcgaccccc gaactgctgg tgcgccgcga gcgcgggctg gtcacctcgc gggtgctcgc
    15721 cgggacgatc cgacgcacgg gtgacgacgc caaggatctc tcgctcgccg cgtcgctggc
    15781 ccgctccagc aaggacctcg aggagcacga atacgccgtg cgctcggtcg cggatgccct
    15841 tgccccgcac tgctcgtcga tgaacgtccc cgagtcgccg ttcgtcctgc acctgcccaa
    15901 cgtcatgcac ctgg
//
LOCUS       DAORPW010000045        15761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_732_length_15761_cov_731.928435, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000045 DAORPW010000000
VERSION     DAORPW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15761
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_732_length_15761_cov_731.928435"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLB86_05640"
     CDS             complement(<1..258)
                     /locus_tag="PLB86_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRC64651.1"
                     /translation="MTRASTPDAPVTPVAVQDSYPPDVAVCYGCGRLNDDGLHIRTFW
                     DGTEGTATFQPKPFHTAITGVVYGGLLASLIDCHAVGTAAAA"
     gene            298..1464
                     /locus_tag="PLB86_05645"
     CDS             298..1464
                     /locus_tag="PLB86_05645"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRC64652.1"
                     /translation="MSRPVGNPALAPFGTTIFATMSALALRHGAINLGQGFPDSDGPQ
                     EVLDAAVAAIRGGRNQYPPGPGTPELLAAIAAHQQRFYGIELDPSREILVTVGATEAI
                     AATILALVQPGDEVVTFEPYYDSYAASIALAGGVRRTSVLRFPDFAVDEASLRAAFSA
                     RTRMVLLNTPHNPTGKVFTRAELDLVCALAREHDAWVVTDEVYEHLIFDGVEHIAVST
                     LPGMWDRTLTISSAGKTFSTTGWKVGWVSGPAEAVAAVRAVKQFLTYVGSGPFQPAVA
                     TGLGLPDEVYAGLAGSLARKRDILCDGLAAAGLPVSRPAGTYFVVADAAALGATDALE
                     FCHWLPEAAGVVGVPVSVFHDDPDAARTLVRFAFCKRDEVLTEAVRRLAGLRVG"
     gene            1578..2162
                     /locus_tag="PLB86_05650"
     CDS             1578..2162
                     /locus_tag="PLB86_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRC64653.1"
                     /translation="MQWFASVGLVLALGVGAAAANTPAAVIQPRPPTAGTQGTAVTAV
                     TAVTPGTALAPGTAVSRWVWPIEPNHVVVHGFDPPATRWSSGHRGVDIAAAVGTSVLA
                     PTDGQVSFVGMVAGRPVLVLTHAGGLRSTFEPVSTTAAVGQLVRRGEAVGTVAAYPGH
                     CAPASCLHWGVLRGATYLDPLALLAGRVILLPMR"
     gene            complement(2171..3166)
                     /locus_tag="PLB86_05655"
     CDS             complement(2171..3166)
                     /locus_tag="PLB86_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HRC64654.1"
                     /translation="MAPTETALRRADLRAAGSTAPASDVTTTRRAANRRRSARRRRMH
                     RLRRTATLALGLLFVGTVVARGAQDPAVALPRGVQSASSSTALPGTLGSAADVARAGT
                     APAADKAVPPTASTSTASELAPSESMGEVEEGAPEPTAAPGAALAPVQASAKGTMHPI
                     AIPDAPWASAGRAVRLSIEVEDGLSIDEALFAATVAGILSDPQGWQTADGVRFVPVSP
                     AQIAAGAGVDVRVTLATPTLTGKLCGPLNVTAANVSCWWGGRAVLNLHRWVRGSATYG
                     TDLTSYRTYLVNHEVGHGLGHRHVSCPAPGRPAPIMVQQTLGLEGCRAWPWPTQQ"
     gene            complement(3288..4292)
                     /locus_tag="PLB86_05660"
     CDS             complement(3288..4292)
                     /locus_tag="PLB86_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRC64655.1"
                     /translation="MPWPEMCDGWDVHEDEVGPQSDAPAQAGLEGAAWAGTVLVDYAR
                     YLDAERGLSVHTSRAYLGDIRALLGYLGRSRGVTALSEIELGDLRGWLAAMSRESSAA
                     RTSVARRAASARTFFRWATRTGRISVDPSLRLVAPRRTGHLPGVLRQSEATELMAVAG
                     VAADDGDPISARDRAMVELLYASGLRVSELAGVDVDDVDFTVGVVRVLGKGGKERVVP
                     FGLPAARALREWAAYRPLLATERSGAALFLGRRGRRVDPRQVREAVHRLVAHVPGAPD
                     LAPHGLRHSAATHLLEGGADLRTVQELLGHASLATTQIYTHVSAERLRKSYEQAHPRA
                     "
     gene            complement(4296..5369)
                     /gene="dprA"
                     /locus_tag="PLB86_05665"
     CDS             complement(4296..5369)
                     /gene="dprA"
                     /locus_tag="PLB86_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRC64656.1"
                     /translation="MDALAAVVENRPWAPEALRTRVAGLDLDEVVQFARRQGWRMVVP
                     GDAEWPSGLDALGAPPIGLWVDGPADLGGVCIGSVAIVGARACTAYGMGVASELGAGL
                     PDAGYAVVSGAAFGIDRAAHAGALSVRGTTVAVLAGGLDRPYPVSNSELIAKIRATGA
                     LVSEVAPGGAPTRSRFLQRNRLIATMTVGTIVVEAGLRSGSLHTAGQAADHGRPVGVV
                     PGPVTSRVSAGCHQWIRDGKGVLVTDVAEVLDLVGRLGVDAAPHRSGPVRPEDELPPQ
                     DRLVLAALPVRAPAAIPTVAAHAGLDVGQTLASLGRLELAGVAVRVGDRWKQTPGRLT
                     QSPDAEAVADVGSREVDAPATPG"
     gene            complement(5509..7047)
                     /locus_tag="PLB86_05670"
     CDS             complement(5509..7047)
                     /locus_tag="PLB86_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRC64657.1"
                     /translation="MGLGRTRSVGLRGVEGFLVEVESDVAPGLPAFTVSGRADAACAQ
                     APDRVRAAASNSQHPIPQRRITVNLSPASVLKVGSGFDLAIVVATLAAAGEIPAGVVE
                     EVVHIGELGLDGSVRPVRGLVPLVVAAAKAGVRHVVVPVGNAREARLVAGVQVHPVAW
                     LEELVARYRTLAHGGPVSDVPEPDALSEPERPAPDLADVVGQAEGRLAVEIAAAGAHH
                     LYLVGPPGAGKSMLAERLPGLLPELDDHQAMEVTAIASILGTLASFDGLCRRPPFVAP
                     HHGASMPAIIGGGSGHVLPGAVTAAHHGVLFLDEAPEFRPGVMQALRQPIESGHVVVA
                     RAIGNIRFPCEFQLVLAANPCPCGMGSGKASSCSCTPIQQRRYSGRLSGPILDRIDIQ
                     VHVPAPSRAALLGEPGESSEVVRARVFQARATASLRWDARPYAVNGRVPGPVLRSGPF
                     RLGPTRTVDLDRAIDRGLLSLRGYDRCLRLAWTIADLAGRTSPTRDDVGLALSLRHQP
                     ALAA"
     gene            complement(7049..7444)
                     /locus_tag="PLB86_05675"
     CDS             complement(7049..7444)
                     /locus_tag="PLB86_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRC64658.1"
                     /translation="MQTSEGMPHRVAAAVGDYGERMAERYLRDQGLEILARNWRCALG
                     EIDIVARDGGCLVVCEVKTRRGVAFGGPIQAVTLRKLARLRRLAAAWLHEREGDAAGS
                     HFPDVRVDVVGVARPRSGPCVIEHLVGVA"
     gene            complement(7548..7853)
                     /locus_tag="PLB86_05680"
     CDS             complement(7548..7853)
                     /locus_tag="PLB86_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HRC64659.1"
                     /translation="MSSEDLEKYETEQELSLYREYRDVVSLFSHVVETERRFYLCNSV
                     DMKVRSDGGEVFFDITMNDAWVWDIYRPARFVKSVRVVTFKDVNIEELPKSELEMPS"
     gene            complement(7850..>8533)
                     /locus_tag="PLB86_05685"
     CDS             complement(7850..>8533)
                     /locus_tag="PLB86_05685"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRC64660.1"
                     /translation="QGYRLLAGMDEVGRGALAGPVSVGVVVIDEVCRSAPAGVRDSKL
                     LSAPARERMVAPIRRWAHAHAVGHASPVEIDQIGIIAALRLAGVRALAALPVTPDLVI
                     LDGNHDWLTAPEQVGLLGFMQDDESEATRTPPVTTMIKADLRCSSVAAASVLAKVERD
                     GLMVALGGEHPAYRWEINKGYAAPEHLAALDLLGPCDLHRRSWRLRGPGSEVVERTDP
                     TDSAPMRTR"
     assembly_gap    8534..8633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8634..9363)
                     /gene="lepB"
                     /locus_tag="PLB86_05690"
     CDS             complement(<8634..9363)
                     /gene="lepB"
                     /locus_tag="PLB86_05690"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRC64661.1"
                     /translation="MTDESPPAAPGSDLVQRGFWRSVWVGVREVVIVVSLAMALSLVV
                     KTFLVQPFHIPSGSMEDTLIKDDRVLVSKLTPGPFELRRGDIVVFTDPGHWLGEIPEV
                     HRTPVQSALIFLGLAPDDSHEHLIKRVIGLPGDHVVCCTVGGQLTVNGVAITEPYLKP
                     GDNAAGGRGSFDIIVPAGKVWVMGDHRSDSADSRFHDDGTGSTGSVPIADIRGKAVLV
                     VWPIDRWAGLSEFGDVFATVPDPGK"
     gene            complement(9360..10073)
                     /gene="lepB"
                     /locus_tag="PLB86_05695"
     CDS             complement(9360..10073)
                     /gene="lepB"
                     /locus_tag="PLB86_05695"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRC64662.1"
                     /translation="MTRREVREGRARSKRAAGDRIPESVKIGVIAIAVMLLVRALVVQ
                     TFSVPSGSMQETLEPGDRILVSRLQRGPSIERGDVVVFDGTDTWGKPAGSDTASGPRQ
                     LLTSILAIVSLSSGADYVKRVVGVPGDHVVCCDVDGRLTINGVAVDEPYLYPGNPPST
                     TRFDVTVPAGKIWVMGDHRSASADSRSQMGKPGGGMVPLDDVVGRAWVRYWPLDRLGS
                     LTPAPTLHNLPLPTGAAPS"
     gene            complement(10136..10513)
                     /gene="rplS"
                     /locus_tag="PLB86_05700"
     CDS             complement(10136..10513)
                     /gene="rplS"
                     /locus_tag="PLB86_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRC64663.1"
                     /translation="MHQLDDVNAATLRDDIPAFRAGDTLKVHVKVIEGTRSRIQVFQG
                     VVIRRHGAGVGETFTVRKVSFGVGVERTFPVHTPIIEKIEVVTRGDVRRAKLYYLRDL
                     RGKAAKIKEKRDTHGKFEAKNGA"
     gene            10830..11216
                     /locus_tag="PLB86_05705"
     CDS             10830..11216
                     /locus_tag="PLB86_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC64664.1"
                     /translation="MSVTPPTRSTPIRFGRTAPGIPVRDIEQALAFYRDALGMTVTFT
                     NGDPVGFVILERDAAEVHLTLAPTHRAGTHNVAHLMVSDAAAVHDRLVEHGARIVKGL
                     RDHDFGLRAFVVADPDGNRLDIAEEL"
     gene            complement(11223..>11789)
                     /gene="trmD"
                     /locus_tag="PLB86_05710"
     CDS             complement(11223..>11789)
                     /gene="trmD"
                     /locus_tag="PLB86_05710"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRC64665.1"
                     /translation="DSPYGGGAGMVMRPEPWGEALDALVASAPDAAPPRLLVPTPSGM
                     PFTQAMARDLAAEPWLAFACGRYEGIDERVMEYAAQRMPVTLVSLGDYVLNGGEVAVL
                     AIVEAVARLLPGVIGNAESLVEESHEDGLLEYPVYTKPASWRGLDVPDVLLSGHHGAI
                     AAWRQAQRVERTATRRPDLLPPDRLPPD"
     assembly_gap    11790..11889
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11890..12338)
                     /gene="rimM"
                     /locus_tag="PLB86_05715"
     CDS             complement(<11890..12338)
                     /gene="rimM"
                     /locus_tag="PLB86_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRC64666.1"
                     /translation="MAHVVARIGKPHGIRGEVTVQLHTDDPAGRFAVGTVLPTKAPKG
                     SGVPLELTIASSRDHNGVWLLGFEEIPDRTGAEGLRGTRLLTPEGTEELLEPDQGGDA
                     WYEEQLVGLAVVLKDGSAVGTVAGLDIGPGQDRLRVARDGGRTAYIP"
     gene            complement(12353..12592)
                     /locus_tag="PLB86_05720"
     CDS             complement(12353..12592)
                     /locus_tag="PLB86_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRC64667.1"
                     /translation="MLEEALEHLVKGIVDHADEVVVRRKELRRGELLEVRVHPEDLGR
                     VIGRSGRTASALRTVMSALSGGSNIRVDIVDTDRR"
     gene            complement(12595..13077)
                     /gene="rpsP"
                     /locus_tag="PLB86_05725"
     CDS             complement(12595..13077)
                     /gene="rpsP"
                     /locus_tag="PLB86_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRC64668.1"
                     /translation="MAVKIRLKRMGKIRTPFYRVVVMDSRTKRDGRAIEEIGKYHPTE
                     EPSVIDIDSARAAYWLAQGAQPTEAVEALLKVTGDWQKFKGIDGTEGTLRVAAPKTSK
                     KDLYEAAIAAAGKSLELKGSATTVRRKPAKAEAAVAEITEALAEADKPAEAAAEGEEA
                     "
     gene            complement(13272..>13697)
                     /locus_tag="PLB86_05730"
     CDS             complement(13272..>13697)
                     /locus_tag="PLB86_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64669.1"
                     /translation="KELGSGASRALWALLAASPAGAVVESWLPPTRDTAYLVDGLRAA
                     GLDPTAVPEVFCDVPPEVARERDRLRAAKGERHPVHRGPVGEGEWRESVAPAAYPVGV
                     GPVLRVDTSQPVTDAQVCRLALAIRAAATPTGSRYSAVI"
     assembly_gap    13698..13797
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13798..14886)
                     /locus_tag="PLB86_05735"
     CDS             complement(13798..14886)
                     /locus_tag="PLB86_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC64670.1"
                     /translation="MATVLHIVGEILVGPADTRPECWVLDGRVTYAAPTGPGHDVQTV
                     RGWALPGLVDAHCHVGLDHHGPVEPDVAERQATADRDAGTLLIRDSGSPADTRWIDDR
                     DDLPRIVRAGKHIARTRRYIRNYAHEIEPDELVAHVRMEARRGDGWVKLVGDWIDRET
                     GDLAPCWPREAVIAAVAAAHEEGARTTAHCFAEESLHDFAAAGTDCIEHASGLDEEST
                     KAFAAQGIAIVPTLINIANFPELAAPAAEKFPRYHAHMLDLHARRFETIARAHEAGIP
                     IYVGTDAGGVLPHGLAAAEALELAKAGLSTLDVLGAGSWKAREWLGHEGIEEGASADF
                     VVYPADPRADLAVLGAPDAIILRGNRVR"
     gene            complement(14907..15746)
                     /locus_tag="PLB86_05740"
     CDS             complement(14907..15746)
                     /locus_tag="PLB86_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRC64671.1"
                     /translation="MTAATSRRLHTLAAGTSGPRIAFLHGLFGQGRNWSQIAKALSGP
                     DGDQAQCLLVDLPDHGHSPWSDHFSLQAYAASVAATLRATAPGERWTLVGHSLGGKVA
                     MIVALSQPDLIERLAVIDIAPRHYRSHQWFVDYVNVMRALPLDSLPDRASANAALAQH
                     IPDPAVRAFLLQNLRRHGTAWSWQINFDVLARPGTGPAGVAGWPTADATAYQPFRGPV
                     AWVAGAESTYITDRDAEPMRALFPRVTQVTVKDAGHWVHSDQPEVVTQILRRFALSTA
                     RRH"
BASE COUNT         2322 a         5491 c         5423 g         2225 t
ORIGIN      
        1 ggcggcggct gccgtgccga ccgcgtggca gtcgatgagc gacgcgagca gcccgccata
       61 gacgacgccg gtgatggccg tgtgaaacgg tttcggctgg aaggtcgccg tgccctcggt
      121 gccgtcccag aaggtccgga tgtgcaggcc gtcgtcgttg agccggccac agccgtaaca
      181 gaccgcaacg tccggtgggt aggagtcctg gaccgcgacg ggtgttacgg gcgcatcagg
      241 cgtcgacgca cgagtcatgg ggacacgcta gccgggcgag cggcatacgg tgagggcatg
      301 agccgtcccg tcggaaaccc ggcactcgcg cccttcggca ccacgatctt cgccacgatg
      361 tcagcgctcg cgctgcgaca cggggcgatc aacctcgggc aggggttccc cgactccgac
      421 gggccgcagg aggtgctcga cgccgcggtg gcggcgatcc ggggcgggcg caaccaatac
      481 ccgcccggcc cgggaacgcc tgagctgctc gccgcgattg cggcacacca gcagcggttc
      541 tacgggatcg agctggaccc ctcgcgggag attctcgtga ccgtcggggc gaccgaggcg
      601 atcgccgcga cgatcctcgc gctcgtgcag cccggcgacg aggtggtcac cttcgagccc
      661 tactacgact cgtatgccgc gtcgatcgcc ctcgccggcg gggtgcggcg gacgtcggtg
      721 ctgcgcttcc ccgacttcgc ggttgacgag gcgtcgttgc gcgcggcgtt ctcggcacgc
      781 acgcggatgg tgctgctcaa caccccgcac aacccgacgg ggaaggtctt cacccgcgcc
      841 gagctcgacc ttgtgtgtgc cctcgcgcgt gaacacgacg cgtgggtggt gaccgacgag
      901 gtctacgagc acctcatctt cgacggcgtc gagcacatcg cggtgtcgac gctgccgggg
      961 atgtgggatc ggacgctcac catctcgtcg gccggcaaga ccttctcgac gaccgggtgg
     1021 aaggtcgggt gggtgtccgg gccggccgag gcggtggccg ccgtgcgcgc ggtcaaacag
     1081 tttctcacct acgtcggatc cgggccgttc cagcccgccg tcgccaccgg gctcggtctg
     1141 cccgacgagg tgtatgccgg tctcgccggc tccctggcgc gcaagcgcga catcctctgc
     1201 gacgggttgg ctgctgccgg gctgcccgtg tcgcggccgg ccgggaccta cttcgtcgtg
     1261 gctgatgctg cggcgttggg cgccaccgat gcgttggagt tctgtcactg gctgcccgaa
     1321 gccgccgggg tggtcggggt gccggtatcg gtcttccacg acgaccccga cgcggctcgg
     1381 accttggtgc gtttcgcctt ctgcaagcgc gacgaggttc tcaccgaggc ggtgcggcga
     1441 ctggctggcc tccgggtcgg ctgagcccgg gcctgagggc gcccggttta tccacacccc
     1501 gtcgtcggcc ggtcccgtta tccacacgct gggagtgacg gttggacggg cccgaccgga
     1561 tgtcgcaggg tcgaggcatg caatggttcg catcggtcgg cctggtgctg gcgttggggg
     1621 tgggtgccgc cgcggcgaac acgcccgcgg cggtgatcca gccgcgtccg cccaccgcgg
     1681 gcacgcaggg gaccgcggtg accgccgtga ctgcggtgac gccggggacg gccctggcgc
     1741 cggggactgc ggtgagccgg tgggtctggc cgatcgagcc gaaccacgtc gtcgtgcacg
     1801 gtttcgaccc gcccgcgacc cggtggagca gcggacaccg aggcgtcgac atcgctgcgg
     1861 cggtcgggac cagcgtcctc gcgccgaccg acggacaggt gagcttcgtc ggcatggtcg
     1921 ccgggcgtcc ggtgctcgtc ctcacacatg ccggcgggct tcggtcgacc ttcgagccgg
     1981 tgtcgacgac ggccgccgtg ggtcaactgg tgcgccgcgg cgaggcggtc ggaacggtcg
     2041 cggcataccc agggcattgc gcgccggcca gttgcctgca ttggggggtg ttgcgcggcg
     2101 ccacctacct cgatccgctc gccctgctcg ccggacgggt gatcctgctg ccgatgcgat
     2161 agggcgcggg ttactgctgg gtgggccagg gccaggcccg gcagccttcg agcccgaggg
     2221 tctgttgcac catgatcggc gccggtcgac caggggcagg acaagagacg tgtcggtgac
     2281 ccagcccatg gccgacctca tggttgacga ggtaggtgcg gtaggacgtg aggtcggtcc
     2341 cgtaggtcgc cgagccgcgg acccaacgat ggaggttgag cacggcgcga ccgccccacc
     2401 aacacgacac attggctgcg gtgacgttca gcggcccaca caacttgccg gtcagggtgg
     2461 gggtggcgag ggtgacgcgc acatccacgc cggcgcccgc cgcgatctgc gccggagaca
     2521 cgggcacgaa gcgcactcca tcggctgtct gccatccctg aggatcactg aggatcccgg
     2581 cgaccgtcgc ggcgaagagc gcctcgtcga tgctcaggcc gtcttcgacc tcgatgctca
     2641 accggaccgc acgccccgcg ctcgcccagg gcgcgtcggg aatggcgatc gggtgcatgg
     2701 tccccttggc ggaggcctgc accggggcga gcgcggcacc cggggcggcg gtcggttcgg
     2761 gggcgccttc ctcgacctca cccatcgact cggacggggc gagctcggac gctgtgctgg
     2821 tggacgccgt cggggggact gctttgtcgg ccgcaggcgc ggtgccggct cgggcgacgt
     2881 cggccgctga tccgagcgtt cccggtagcg ccgtcgacga actcgcggac tgcacgccgc
     2941 gcgggagcgc caccgccggg tcctgtgctc cccgggctac gaccgtgccg acgaagagca
     3001 accccaaagc caacgtcgcc gtgcgccgca gtcggtgcat ccgacggcgc cgagccgagc
     3061 gccgccggtt ggcggcccgt cgagtcgtcg tcacgtccga cgcaggcgcg gtcgaaccgg
     3121 cggcgcgcag atcggcacgc cgcagggccg tctcggtcgg ggccacgcca ccttctgagg
     3181 gtcgagagag ggaaccaccg ggccgcctgt cagcgcccag gaggcaccat cgtcccagat
     3241 cggtggatcg cgaggggctc gggcacgccg gtgggacgcc tcccggctca ggccctcgga
     3301 tgagcctgct cgtaggactt gcgtagccgt tcggccgaca cgtgcgtgta gatctgcgtc
     3361 gtcgccaggc tcgcgtggcc cagcaactcc tggacggtcc tcaggtcggc cccaccttcc
     3421 aacaagtggg tcgccgcgct gtgtcgcagc ccgtgcggcg ccaggtcggg ggcgcccggc
     3481 acatgagcga ccagccggtg caccgcctca cgcacctggc gcggatcgac gcgccggcca
     3541 cgtcggccga ggaacagtgc ggcaccactg cgctcggttg ccagcagcgg ccggtatgcc
     3601 gcccactccc gaagggctct cgcggcaggc aacccgaagg gcacgacgcg ctccttgcca
     3661 cccttgccca gcacgcggac gacccccacg gtgaagtcga catcgtccac gtctacgcct
     3721 gccagctcgc tcacgcgcag gccgctggcg tagagcaact ccaccatcgc ccggtcgcgt
     3781 gccgagatcg gatcgccgtc gtcagctgcg actccggcga ccgccatgag ctcggtggcc
     3841 tccgactgtc gcaacacccc gggcaggtgc cccgtgcgac gtggcgccac caaccgcagc
     3901 gacgggtcga cggagatccg tccggtgcgg gtcgcccacc ggaagaaggt ccgcgcgctg
     3961 gcggcccgac gcgcgacgct ggtgcgagcc gcgctggact cccgggacat cgcagccagc
     4021 cagccccgca gatcccccaa ctcgatctcg ctcagcgccg tcactccccg cgaccggccg
     4081 aggtagccca gcaaggcccg aatgtcgccg agataggcgc ggctagtgtg cacggacagc
     4141 ccgcgctcgg cgtcgagata gcgcgcgtag tcgacgagca cggtgcccgc ccatgcggca
     4201 ccctcgagcc cagcctgagc cggtgcgtcg ctctgggggc ccacctcgtc ctcatgcacg
     4261 tcccaaccgt cgcacatctc tggccacggc atgcctcacc cgggggttgc tggcgcgtcc
     4321 acctcccggc tgccgacgtc cgccacagcc tcagcatcgg ggctctgcgt cagccgtccg
     4381 ggggtctgct tccaccggtc gccgacgcgc accgcgaccc cagccaactc cagccgcccc
     4441 agggaggcca gcgtctgacc gacgtcgagg cccgcgtggg cagcgaccgt ggggatcgct
     4501 gccggtgctc gaaccggaag cgcagccaac accaaccggt cttggggagg caactcgtcc
     4561 tccgggcgaa cggggccgga gcggtggggc gcggcgtcga cgcccagccg ccccacgaga
     4621 tcgagcacct ccgctacgtc ggtgacgagc actcccttcc cgtcgcggat ccactgatgg
     4681 cagcccgcag acacccgcga ggtcaccggc cccggcacca cgccgaccgg tcttccgtgg
     4741 tcggctgcct gcccggcggt gtgcagcgac cccgaccgca atccggcttc gacgacgatc
     4801 gtcccgacgg tcatcgtggc gatgagccgg ttgcgctgca ggaagcgcga tcgggtcggt
     4861 gccccgcccg gagccacctc ggacaccaga gccccggtcg cgcggatctt ggcgatcaac
     4921 tcggagttac tgaccgggta gggccgatcg aggccccccg cgagcacggc aaccgtggtc
     4981 ccgcgcaccg acagcgcgcc cgcgtgggcg gcgcggtcga tgccgaacgc ggcaccggac
     5041 accacggcat accccgcgtc gggaaggccg gcccccagct cggaggcgac ccccatcccg
     5101 tatgccgtgc acgcccgggc cccgacgatc gccaccgacc cgatgcacac gcccccgagg
     5161 tcagccgggc cgtcgaccca caggccgatc ggtggagcgc cgagagcatc gagaccgctc
     5221 ggccattcgg cgtcgcccgg cacgaccatc cgccacccct ggcggcgggc gaactgcacc
     5281 acctcgtcga ggtcgagccc agccaccctg gtccgcagcg cctcgggagc ccacggacgg
     5341 ttctcgacga cggcggccaa cgcgtccacg gcgccgagtg tctccacgag gccaacggcc
     5401 cgacgatctc ccggctcggc gacccgggac caggccgccc tagcccaccg gtcttgggtc
     5461 aaggaggtcg gcaagacact catgaggccc cctgacagtg ccgggcggtc atgcggcgag
     5521 ggcgggttgg tggcgcagcg acagggccag acccacgtcg tcgcgggtcg gcgaggtgcg
     5581 cccggccagg tcggcgatcg tccacgccag tcgaaggcaa cggtcatagc cccgcaacga
     5641 cagcaggcca cggtcaatcg cgcggtcgag atcgacggtc cgtgtcgggc cgagccggaa
     5701 ggggcccgat cgcaggacgg gaccggggac ccgaccgttg acggcatacg ggcgggcatc
     5761 ccaccgcagc gacgcagtcg cgcgggcctg gaagacccgg gctcgcacca cctcgctgga
     5821 ctcccccggc tcacccaaca aggccgcccg cgagggcgcc gggacgtgga cctggatgtc
     5881 gatccggtcg aggatggggc cggacaaccg acccgagtag cgacgctgtt ggatcggcgt
     5941 gcacgaacac gaggaggcct ttcccgaccc cattccgcac gggcaggggt tggccgcgag
     6001 gacgagttgg aactcgcagg ggaaccggat gttgccgatc gcccgggcca ccaccacgtg
     6061 gccggactcg atgggttggc gcagggcctg catcaccccg ggacggaact cgggtgcctc
     6121 atcgaggaag agcacgccgt gatgagccgc ggtcaccgct ccgggcaaca catgcccgga
     6181 ccctccaccg ataatcgccg gcatcgacgc gccgtggtgc ggcgcgacga acggcgggcg
     6241 tcggcacagg ccgtcgaagc ttgcgagggt gccgaggatg gaggcgatgg cggtgacttc
     6301 catcgcctgg tggtcgtcca actccggaag caggcccggc agtcgctcgg cgagcattga
     6361 cttgcccgcg ccgggcggac ccacgaggta gagatggtgc gcgccagcgg cggcgatctc
     6421 gacggcgagg cgcccttcgg cctggccgac gacgtcggca aggtcaggtg ctggacgctc
     6481 gggctccgac agggcgtccg gctcgggcac gtcggacacc ggcccaccgt gcgccagcgt
     6541 ccgatatctc gcgacgagct cctcgagcca ggccaccggg tggacctgca ccccggcgac
     6601 gagccgagcc tccctggcat tgcccacagg aacgaccacg tgccgcacgc cggccttggc
     6661 ggcagccacg accaacggga ccaaccctcg gaccggtcgg accgacccgt ccagacccag
     6721 ctcaccgatg tgcacgacct cctcgacgac gccggcgggg atctctccgg cggcggccaa
     6781 ggtcgcgacg acgatggcca gatcgaaccc actgcccacc ttgagcaccg acgccggcga
     6841 caggttgacg gtgatccgtc gttgcgggat cgggtgctga ctgttggagg ctgccgcccg
     6901 cacccgatcg ggtgcctggg cacaggccgc gtcagcccgt ccactcacgg tgaacgccgg
     6961 cagccccggc gcgacgtcgg actcgacctc gacgaggaac ccctcgacgc ctcgcagccc
     7021 cacagatctc gtccgcccca accccatctc aggccacccc cacgaggtgc tcgatcacgc
     7081 aggggccgct gcgcggtcgg gccaccccga cgacatcgac ccgcacgtca gggaagtgcg
     7141 acccggccgc atcgccctcg cgctcgtgca gccacgcggc agccaatcgg cgcagccgcg
     7201 cgagcttgcg caacgtcacc gcctggatcg ggccaccgaa cgccacccca cggcgggtct
     7261 tgacctcgca cacgaccagg caacccccgt cgcgcgccac gatgtcgatc tcgcccaagg
     7321 cacagcgcca gttgcgggcc aggatctcca aaccctgatc gcgcaaatag cgctcggcca
     7381 tccgctcgcc atagtcaccg accgcggcgg cgacccggtg cggcataccc tccgatgtct
     7441 gcatgcccga agcgaaacag ccccgcgggt atgccgccca cccccacctc ctgccctgtg
     7501 gacagtggga cgaacgtacg accccatgtg gacagccggg ccgatcgcta ggagggcatc
     7561 tccaactcag acttgggcag ttcctcgatg ttgacgtcct tgaacgtcac cacccgcacc
     7621 gacttgacga accgcgctgg ccgatagatg tcccacaccc acgcatcgtt catcgtgatg
     7681 tcgaagaaca cctcgccccc gtcggagcgc accttcatgt cgaccgagtt gcacaggtag
     7741 aagcgccgct cggtctcgac cacgtgggag aacagcgaca cgacgtcgcg gtactcgcgg
     7801 tagagcgaga gttcctgctc ggtctcgtac ttctccaggt cctcggagct catcgggtcc
     7861 tcatcggcgc gctatcggtc gggtcggtcc gttctaccac ctcggagcca ggtccacgca
     7921 gccgccaact gcgccggtgc aggtcgcagg gccccagcag gtcgagcgcg gcgaggtgtt
     7981 cgggcgcggc ataccccttg ttgatctccc accggtatgc cgggtgctcg cctcccagcg
     8041 ccaccatcag accgtcgcgt tccaccttcg ccagcaccga cgcggcggcc actgaggagc
     8101 agcgcaggtc ggccttgatc atcgtcgtca ccggcggcgt ccgggtggct tcggactcgt
     8161 cgtcctgcat gaaccccagc aacccgacct gctcgggtgc ggtcagccag tcatggttgc
     8221 cgtcgaggat gaccaggtcg ggggtcacgg gcagcgccgc gagcgcgcgg acgccggcca
     8281 gccgcaacgc cgcgatgatc ccgatctggt caatctccac gggcgaggca tgccccaccg
     8341 cgtgcgcgtg ggcccaccga cggatcgggg cgaccatgcg ttcgcgggcc ggggcggaga
     8401 ggagcttgga gtcgcggacc ccggcggggg cggagcggca tacctcgtca atgacgacca
     8461 cgcccacact caccgggccg gccagggcgc ctcgacccac ctcgtccatc ccggccaaca
     8521 accggtagcc ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccttgcc
     8641 cggatcgggc acggtcgcga acacgtcgcc gaactctgac agcccggccc accgatcgat
     8701 cggccagacg acgagcaccg ccttgccgcg gatgtcggcg atcgggaccg accccgtgga
     8761 gccggtgccg tcatcgtgga atcgggagtc ggccgagtcg gaccggtgat cccccatgac
     8821 ccagaccttg ccggcaggga cgatgatgtc gaaggaaccc cggccgcctg ccgcgttgtc
     8881 gccgggcttg aggtagggct cggtgatggc gacgccgttc acggtgagtt gcccacccac
     8941 ggtgcagcag acgacatggt cgcccggcag cccgatgacg cgcttgatga ggtgttcgtg
     9001 cgagtcgtcg ggggccaacc cgaggaagat cagcgccgat tgcaccgggg tgcggtgaac
     9061 ctcggggatc tccccgagcc aatggcccgg gtcggtgaag acgacgatgt ccccccgacg
     9121 caactcgaac ggcccgggcg tcagcttgct gaccaggacc cgatcgtcct tgatgagggt
     9181 gtcctccatc gacccggagg ggatgtggaa gggctgcacc aggaaggtct tgacgacaag
     9241 ggagagcgcc atcgcgaggg acaccacgat gacgacctcg cgaacaccca cccagacact
     9301 gcgccagaag ccgcgctgca ccaggtcgga tccgggtgcg gcgggcggag attcgtcggt
     9361 catgaaggcg ccgctccggt gggcagtggg agattgtgca gtgtgggggc aggagtcagg
     9421 ctacccagtc gatcgagcgg ccagtagcga acccacgcac gtccgacaac atcgtcgagg
     9481 ggaaccatgc cgccgcccgg tttgcccatc tgtgatcgcg aatcggccga cgcactgcgg
     9541 tggtcgccca tcacccagat cttcccggcc ggcaccgtaa cgtcgaacct ggtggtgctc
     9601 ggcgggtttc cggggtagag gtacggctca tcgacggcca ccccgttgat ggtcagccga
     9661 ccgtcaacgt cgcagcacac aacgtggtcg cccggcacgc cgacgacccg cttgacgtag
     9721 tcagcgcccg aactcagcga cacgatggcc aagatcgagg tcagcagttg acggggcccc
     9781 gaggcagtgt cggacccggc aggcttgccc cacgtatcgg tcccgtcgaa gacgacgacg
     9841 tcgccgcgct cgatcgacgg ccctcgctgc agccgactga cgaggatccg gtcgcccggc
     9901 tcgagagtct cttgcatcga gcctgagggg acggagaagg tctggacgac cagcgcgcgg
     9961 acgaggagca tgacggcgat cgcgatcacg ccgatcttga ccgactcggg gatgcggtcg
    10021 ccagcagcac gcttgctgcg tgcccgccct tcacgcacct cgcggcgtgt caacggccgc
    10081 gcgttgccac catcaggctc acgcggcagg ccccccgagg cctcgtccac tcgcctcagg
    10141 cgccgttctt ggcctcgaac ttgccgtggg tgtcgcgctt ctccttgatc ttggcggcct
    10201 tgccgcgcag atcgcgcagg tagtagagct tcgcgcggcg gacgtcacca cgggtgacga
    10261 cctcgatctt ctcgatgatc ggggtgtgca ccgggaaggt ccgctcgacc ccgacgccga
    10321 aggacacctt gcgcacggtg aacgtctcgc ctacgcccgc gccatggcga cggatgacaa
    10381 cgccctggaa gacctggatg cgggagcggg tgccttcgat gaccttgacg tgcaccttga
    10441 gggtgtcacc ggcgcggaag gcggggatgt cgtcgcgcag agttgcggcg ttgacgtcat
    10501 caagctggtg catgggatca tctgctttct cgcggcgcca caggtcgcct cggggggtgg
    10561 ctggttcaga gctttcggta tgccgtccgg ctggccgcgc gacggccctc ccgaagatcg
    10621 ggtggtccgg atcgctcccc ctgtggcagg ggctcgtcag atgctggaag gcaactgccc
    10681 aagtgtgcca gaccacgcgg cataccggga aatcatggct aggatcaccc cgtcatcgcg
    10741 ggcaccgccc cgtctcccgc cgctcgcctg gttgggcacc gcctcagcca gggctcccga
    10801 gtcggcgact cgaaagtatg gagaccgcca tgtccgttac tcccccgacc agaagcacgc
    10861 cgatccgctt cgggcgcacc gctcccggca tccctgtccg cgacatcgag caggccctcg
    10921 cgttctatcg ggacgccctc gggatgacgg tgaccttcac gaatggcgac cccgtcgggt
    10981 tcgtcatcct cgaacgcgat gccgccgagg tgcacctcac gctggcgccg acgcaccggg
    11041 ccgggacgca caacgtcgcc cacctcatgg tgagcgacgc ggcggcggtg catgaccggc
    11101 tggtcgagca tggggcgcgg atcgtcaaag gcttgcgcga tcacgacttc gggctgcgcg
    11161 ccttcgtcgt cgccgacccc gacggcaacc gcctggacat cgccgaagaa ctctgagggc
    11221 ccctagtcag ggggaagccg gtcgggaggc agcaggtcgg ggcggcgggt ggcggtgcgc
    11281 tcgacccgct gcgcctgccg ccaggccgcg atcgcgccgt ggtgtcccga gagcaacacg
    11341 tcgggcacgt ccagaccgcg ccaagaggcc ggcttggtat agacggggta ttccagcagc
    11401 ccgtcctcgt gcgactcctc gacgagcgac tcggcgttgc cgatgacgcc gggcagcagg
    11461 cgcgcgacgg cctcgacgat ggccagcacg gccacctctc ccccgttgag gacgtagtcc
    11521 ccgagcgaca cgagcgtcac gggcatgcgc tgcgcggcat actccatgac ccgctcgtcg
    11581 atgccctcgt aacggccgca cgcgaacgcc agccacggct cggcggcgag gtcgcgcgcc
    11641 atggcctggg tgaacggcat accggatggg gtggggacga gcaggcgcgg cggggcggcg
    11701 tcgggcgcgg aagcgaccag cgcgtcgagc gcctcgcccc acggctcggg gcgcatgacc
    11761 atgccggctc ccccgccata gggcgagtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnna acgggatgta tgccgtccgc ccaccatcgc gggccacgcg caaccggtcc
    11941 tgacctgggc cgatgtccag gccggccacc gtgccgaccg ccgacccgtc cttgagcacg
    12001 acggcgaggc cgacgagttg ctcttcgtac caggcgtctc cgccctggtc gggctcgagc
    12061 aattcctcgg tgccctcggg ggtcagcagg cgggtgccgc gcaggccttc ggcgccggtg
    12121 cggtcgggga tctcctcgaa ccccagcagc cagacgccgt tgtggtcccg cgaggacgcg
    12181 atggtgagtt caagcgggac accgctgccc ttgggtgctt tggtgggcag gacggtgccg
    12241 acggcgaacc ggcccgcggg gtcgtccgtg tgcagttgca ccgtgacttc cccgcggatg
    12301 ccgtgcggct tgccgatccg ggcgacgacg tgcgccacga gagggctgag cctcagcgcc
    12361 ggtcggtgtc gacgatgtcg acgcgaatgt tcgagccgcc ggacagggcc gacatgaccg
    12421 tgcgcagcgc gctggccgtg cgacccgagc ggccgatgac tcggccgagg tcctcggggt
    12481 gcacgcgcac ctcaagcaac tcgcctcgac ggagctcctt gcggcgcacg acgacctcgt
    12541 cggcgtggtc gacgatgccc ttgaccaggt gttccagggc ctcttcgagc acggtcaggc
    12601 ctcttcgccc tcggcagcgg cctcggccgg cttgtcggcc tcggcgagcg cctcggtgat
    12661 ctcggcgacg gcagcctcgg ccttggccgg cttgcggcga accgtggtgg ccgagccctt
    12721 gagctcgagc gacttgcccg cggcagcgat ggcggcctcg tagaggtcct tcttgctggt
    12781 cttcggggcg gcgacgcgaa gggtgccttc ggtgccgtcg atgcccttga acttctgcca
    12841 gtcaccggtc accttgagca gcgcctcgac ggcctcggtg ggctgagcgc cctgggccag
    12901 ccagtaggcg gcgcgagcgg agtcgatgtc gatgaccgag ggctcctcgg tggggtggta
    12961 cttgccgatc tcctcgatcg cccggccgtc acgcttggtg cggctgtcca tgacgacgac
    13021 acggtagaac ggggtgcgga tcttgcccat ccgcttgagg cgaatcttga cggccacggt
    13081 agtggtctcc tgcttcgtat cgtgagcacg gcgagaccgg ggcaagtcgc tccggggtgc
    13141 cgtcactcgg cgtgtagatg cacgctccct ggtgggtgcg gcgagtacag ccgcccattc
    13201 tgccagaccc cgcaggtatg ccgcgccacc ccctgccgga ccccctgccg ggtcgaggta
    13261 ttccgccatc gtcagataac ggcggaatac ctcgacccag ttggggtggc ggcggctcgg
    13321 atggcgaggg cgaggcggca tacctgcgcg tcggtcactg gctgggaggt gtcgacccgc
    13381 aacaccggcc ccacacccac cgggtatgcc gcgggcgcga ccgactcgcg ccactcccct
    13441 tcaccgaccg gaccacggtg cacagggtgc cgctctccct tcgcggcgcg caggcgatcg
    13501 cgctcacgag ccacctccgg tgggacgtcg cagaacacct cggggaccgc cgtcgggtcg
    13561 agccccgcag cgcgcaggcc gtccacgagg tatgccgtgt cgcgcgtcgg cggcaaccag
    13621 gactcgacga ccgctcccgc gggcgaggct gcgagcaatg cccagagcgc ccggctcgct
    13681 ccggagccga gctccttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntca
    13801 tcgaactcgg ttgccgcgca ggatgattgc gtcgggggct ccgaggacgg cgaggtcggc
    13861 gcgcgggtcc gcgggataga cgacgaagtc ggctgaggcc ccctcctcga tcccttcgtg
    13921 gccaagccac tcgcgggctt tccacgatcc ggcgccgagg acgtcgagcg tcgacaaccc
    13981 cgccttggcc aactccaggg cctccgcagc ggcgaggccg tgcggcaaca ccccacccgc
    14041 gtcggttccg acatagatgg gtatgccggc ctcgtgcgcc cgcgcgatcg tctcgaaccg
    14101 gcgggcgtgc aggtcgagca tgtgggcgtg atagcgcggg aacttctctg ctgccggtgc
    14161 cgccaactca gggaagttcg cgatgttgat cagcgtcgga acgatcgcga tcccttgtgc
    14221 cgcaaaggct ttggtggact cctcgtccag gccgctggcg tgctcgatgc agtccgtgcc
    14281 tgccgccgcg aagtcgtgca gggactcctc ggcgaagcag tgagcggtgg tgcgggcgcc
    14341 ctcctcgtgg gcggctgcga ccgcggcgat gaccgcctcg cgcggccagc agggcgccaa
    14401 gtcgccggtc tcccgatcga tccagtcacc cacgagcttg acccagccat cgccccggcg
    14461 cgcctccatc cggacgtgcg cgacgagctc gtcgggctcg atctcgtggg cgtagttgcg
    14521 gatgtagcgc cgggtgcggg cgatgtgttt gccggcccgg acgattcgtg gcaggtcgtc
    14581 cctgtcgtcg atccaccggg tgtcggctgg gctgcccgag tcgcggatga gcagggtgcc
    14641 ggcatcccga tcagcggtgg cctgccgctc ggcaacgtcg ggctcgaccg ggccgtggtg
    14701 gtccaggccc acgtgacagt gggcgtcgac gaggccgggc aacgcccagc cccgcacggt
    14761 ctggacgtcg tgaccgggcc cagtgggggc ggcatacgtc acccgcccgt cgagcaccca
    14821 gcactccggc ctcgtgtccg cgggtccgac caggatctcc cccacgatgt gcagcaccgt
    14881 cgccatgggg cgactgtatc cagcggtcag tgcctgcgag ccgtcgacag ggcgaagcgg
    14941 cgcaagatct gcgtgacgac ctcgggttgg tcggagtgca cccagtgccc ggcgtccttg
    15001 accgtcacct gcgtcacccg aggaaacagt gcccgcatgg gctcggcgtc gcggtcggtg
    15061 atgtaggtcg actccgcacc cgccacccag gcgaccggcc cccggaaggg ctggtatgcc
    15121 gtcgcgtcag cggtcggcca gcccgccacc cccgctggcc cggtgcccgg tcgggcgagg
    15181 acgtcgaaat tgatctgcca gctccatgcg gtgccgtggc ggcgcaggtt ctgcaagagg
    15241 aaggcgcgga ccgccgggtc tgggatgtgt tgcgccagcg cagcattggc cgatgcccgg
    15301 tcgggcagcg agtcaagcgg cagcgctcgc atgacgttga cgtagtcgac gaaccactgg
    15361 tgggagcggt agtgcctggg cgcgatgtcg atgaccgcaa gccgctcgat caggtcgggc
    15421 tgcgacagcg cgacgatcat ggctaccttg ccgccgaggg agtgaccgac gagggtccag
    15481 cgttcgcccg gggcggtcgc acgcagcgtg gccgcgactg acgcggcata cgcctggagg
    15541 gagaagtggt cactccacgg tgagtggccg tgatcaggca ggtcaacgag caggcactgt
    15601 gcctggtcac cgtctgggcc cgagagcgcc ttggcgatct ggctccagtt gcgaccttgg
    15661 ccgaagagcc cgtgcaggaa ggcgattcgc gggccggatg tgcccgccgc gagggtgtgc
    15721 agccgcctgc tggttgccgc cgtcatgccg acgagcctaa c
//
LOCUS       DAORPW010000046        15472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_749_length_15472_cov_722.088993, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000046 DAORPW010000000
VERSION     DAORPW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15472
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_749_length_15472_cov_722.088993"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLB86_05745"
     CDS             <1..450
                     /locus_tag="PLB86_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC64672.1"
                     /translation="PDATEEQMRAALEAAQVLHLVDSLPSGLDTVVGDRGHRLSGGEK
                     QRLAIARLLLKAPHVVVLDEATAHLDSESEVAVQRALDVALAGRTALVIAHRLSTVRG
                     ADQILVVEDGRIVERGKHAELLGRGGLYADLYRTQYFEDSTTKAGPW"
     gene            complement(471..1271)
                     /locus_tag="PLB86_05750"
     CDS             complement(471..1271)
                     /locus_tag="PLB86_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRC64673.1"
                     /translation="MWRAIFTRRILYAVAFALGFGTVCFLLGQWQLSRYEEKDARATA
                     VEQNYNRPAVPFAGVLPSVTTPLAESQVWTQVTLTGRYAASTHLIVRGRALNTAQGME
                     ILVPFQVDGGAVLLVDRGWVPNGDQAADLPTVSAPPAGTVTLTGWLKPGEEDRGKDLP
                     AGQLATINLAQAQEQVPGQLYAAYLVLGSESLGEGLPAPARPTPLEAPTPDRGPHFAY
                     ALQWWLTTLLGFGFVVYLYRHPPGGVPNATPTEAPEPKKVRIWDEEDG"
     gene            1304..2335
                     /gene="moaA"
                     /locus_tag="PLB86_05755"
     CDS             1304..2335
                     /gene="moaA"
                     /locus_tag="PLB86_05755"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRC64674.1"
                     /translation="MTRLPDPRPRPPEGSALIDTYGRIAQDLRVSLTDRCSFRCTYCM
                     PAEGLPWIPQPQMLSDDELMRLIGVFVGAGVTQVRLTGGEPLLRRSIVELVAGIAALR
                     PRPAIAMTTNGAALAAVAAPLAAAGLDRVNVSLDTIDASQFFALTRRDRLADVIAGLA
                     AAADAGLTPVKVNAVAMRGVNDAVVADLLAWCLARGYELRFIEQMPLDAQHGWDRSVM
                     VTAEEIKAGLEARFELTPVESTDRGSAPAARFLVDGGPATVGIIASVSEPFCSACDRV
                     RLTADGQVRNCLFSERETDLRGPLRDGASDAELLGLIRGEMWRKGPAHGIGRPDFHQP
                     DRPMSAIGG"
     gene            complement(2352..2702)
                     /locus_tag="PLB86_05760"
     CDS             complement(2352..2702)
                     /locus_tag="PLB86_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HRC64675.1"
                     /translation="MRLGRKPQTPTVVHTVTSAPMSLAQDTDMRMRRYLITMAIRVLC
                     FVLAVVFEGWLRWVFVAGAAVLPYIAVVLANAVGPRWGARISGIDKYEPTHPELSAST
                     PTGEPVLGADPFRR"
     gene            2861..3583
                     /gene="fabG"
                     /locus_tag="PLB86_05765"
     CDS             2861..3583
                     /gene="fabG"
                     /locus_tag="PLB86_05765"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRC64676.1"
                     /translation="MTDAAPGRTVLVTGGNRGIGLAIARSFAEAGDRVVVTTRSGEAP
                     DGLDAVPCEVTDSASVDAAFAAAEGIFGSPVEVLVANAGVTRDQLLLRMSDEDFDSVI
                     DTNLAGAFRCARRASKGMIRLKRGRMIFISSVVGLYGSPGQTNYAASKAGLVGMARSI
                     SRELGGRNITANVVAPGFIDTDMTAALPDERRAAYLASIPAGRFAAPEEVAAVVRFLA
                     SPEAGYVTGAVIPVDGGLGMGH"
     gene            3674..4429
                     /gene="fabI"
                     /locus_tag="PLB86_05770"
     CDS             3674..4429
                     /gene="fabI"
                     /locus_tag="PLB86_05770"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HRC64677.1"
                     /translation="MFDGKTLLITGVLMDTSIAFHVAKLAQEQGASVILTSFGRTMKI
                     TQTIARRLPVTPPVVELDVSNPEHLDTLAERLREHTDRLDGVLHSIGFAPLGAFTFLD
                     ATWEDVGTAVHVSAYSLKALAVAALPLMEPGGSIVGLTFDARYAWPVYDWMGVAKAAF
                     ESTNRYCARDLGPKGIRCNLVAAGPIRTTAAKSIPGFEVFEEQWDTRAPLGWDVTDPL
                     PTAKACVALLSDWFPATTGEIVHVDGGVHAMGC"
     gene            4484..6691
                     /locus_tag="PLB86_05775"
     CDS             4484..6691
                     /locus_tag="PLB86_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC64678.1"
                     /translation="MEPVHADEVRILEGPNLYVTRPAVKVSLALPGVAQLDVHAALDL
                     ARRVGLRTARPGRPDTGQRQRFLMRLVEHLVRRVATESGARRLAVRTRAGRHTEDVVV
                     AVPWRHRGRAIALGESLAPLLSDLLKADADTEALIAAAGRAVAEAPDGEPAAVLTPTV
                     PVIAITGTNGKTTTTRLVAHMVMTAGLRTAWSSTDGVVVMGEMVQPGDYSGPGGAREV
                     LNTPGLQLGILETARGGMLLRGMGVNQTDVTVVTNVAADHLGLNGIDTLDQLAEVKAI
                     PTLIVKPDGWTVLNGDDPRVWAMRHRAKGKIWAFSLDPNAPALREAVAGGGRGITVLD
                     GEIVVLTPGRDPDRLVKVVDVPMTLAGLSSINIANALGGTAAGLGLGLTRAAVVEGLR
                     TFRPDPVLNSGRFNTYSVPLTGGGSATVVIDMAHNEAGLEALLEVCRGLTAPGGRVRL
                     GIGGTGDRLPEANAALGEIAGRGADHVVLAHKLKYLRGMSLEQMDSLLRAGLARSGMA
                     EVAGFLTEIEALTTLLAAAESGDVIGLMCHVQRAEVADLIAQRGGTVDDPRTIRSKVI
                     AARGMHEAEDDIAAIWALTDAGERVTAAGRLATAHPSDPRLAYEWACAIDATADRARA
                     VSLYHRALDNGLREPHRHRAQLQAAAAYRDLGHPEIALELLEAVAATHPGSSVAAALR
                     ALVLVDTGDAAEAVADLVDALMEHATDEDAAAYRTFLHHAAAQLRTDRPGVVD"
     gene            complement(6747..8189)
                     /locus_tag="PLB86_05780"
     CDS             complement(6747..8189)
                     /locus_tag="PLB86_05780"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRC64679.1"
                     /translation="MIAGLLAALVAPLSLRGGPATSGDLDLAARVRAAVEDDRGYQAL
                     HVSEITPTSVRHTGVGSMEPGGAEALGAATPIELGSITKTFNGLLLADAIARGEVAAT
                     DPVSTYLPRLAGSPIGGVTLEELASHRGAVPPFPPPTMARGLWGLLSNTDQLSGDQLD
                     WVLDQAAAMPLFGERGTVQYSNLGATLLGWALASAAQQPDWQTYVTTRLLTPLAMTHT
                     RFAATRDQIPPDAPRGRLVGGRQAVQGVSPVYQPAGSSTWTTPEDIARYAQAILRGTA
                     PGLPALDPRFAGEQAASPGNPRVGYHWFTLTVAGRTVQWHNGATAGYTSMLVIDRLAG
                     RAVFVVGNSTRPVDPLAIRLLTGVADGPGSTDAPMPVIAVAATTVALVLIAGAGFAAY
                     RARTRLQLVRATAGALLGLSLWRVAGPWDHAPGLLWILVAAATAATVMLGIARVRTRP
                     WHRPRLAALSWAGAVLALAAAGLVLSMGLH"
     gene            complement(8398..11265)
                     /gene="cphA"
                     /locus_tag="PLB86_05785"
     CDS             complement(8398..11265)
                     /gene="cphA"
                     /locus_tag="PLB86_05785"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRC64680.1"
                     /translation="MAKDHPTPTGPIAPDLRILETRVYRGPNVWSYAPAIHLVVDLGV
                     LEGYPSDSIDGFTDALVALLPGLQRHTCSVGHAGGFIERLEDGTWLGHVAEHVALELQ
                     STAGHDLRRGKTRMVKGRPGVYNVIFGYVDETVGIAAGELAVRLINHLVQPTDGFDFT
                     EQLDAFLRRADRVAFGPSTAAIIEEAVSRDIPWLRLNSASLVQLGQGVHAQRIRATMT
                     SRTGALAVDIASDKDLTGKLLSAAGLPTPKQQAVRTRRGVLDAARQIGFPVVVKPLDG
                     NHGRGVCLDLQTLDEVDKAYDIAQEQSRSGVLIVESFVTGRDYRCLIVGGRMQAIAER
                     VPAHVVGDGEHTVKELVDITNSDPRRGVGHEKVLTRIKVDAAAIEVLEGQGYLLTDVP
                     DAGVMVKLALTGNMSTGGISVDRTFDAHPDNVEIAEEAARMVGLDVAGIDFICPDIAA
                     PVRETGGAIVEVNAAPGFRMHTHPTVGVPQFIAKPVVDLLFPPGAPSRVPIVAVTGTN
                     GKTTTSRMIAHIFKGLGRKVGMTSTDGVVIDERLVIKADASGPKSARMVLMNPRVDFA
                     VMEVARGGILREGLGYDRNDVAVVTNVAPDHLGLRGIDTLAQLAAVKAVVVEAVPKNG
                     FAVLNADDPHVRSMRRKCSGEIVWFTLAAPGSDIREFVDAHCRRGGRALYLDPTDRGD
                     MIVIQHGRRAMQLAWTHLLPATFGGTARFNVANALAAAGAAFAAGAPLHDIRQGLRTF
                     TSNYYLSPGRLNLVEVNNVEVFVDYCHNAAGMRALGDFVDRYAATRAGRADLGRVSRI
                     GAIGAAGDRRDSDLRELGAVAAEHFDVLVIREDARLRGRRPGEAAQLIREGVKEAMQK
                     GVRCRQVDIVLDELQAVGDVMARANPGDLVVVCVDNSAEVFAALEDMTRQAQPGAHPG
                     GVSDPDFDPVVLTGAAAVSGAAAEGRALSTPVPETTGAPNA"
     gene            complement(11309..12253)
                     /locus_tag="PLB86_05790"
     CDS             complement(11309..12253)
                     /locus_tag="PLB86_05790"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRC64681.1"
                     /translation="MVSNAGPADEAGMPPTSPPVRRTLLIVGGAEDKLGRSVVLRRFV
                     RLAGGRASRIVVIPTASSFSTEASALYRSVFARLKAGQDFEIVHPPTRAAASSAELVA
                     RLDAATGIFMTGGSQLKLSQNIVGTPVGDAIHRAYQRGAVIAGTSAGASIMSQFMISL
                     GDEGVTPRQRSSQLSAGLGLVTGVVVDQHFDQRGRYGRLLSIVANSPNLIGIGIDENT
                     AAEIRDERLMSVVGAGAVFVLDARHAITDAPEARRGAPLLVSGAVVHTLPAGATFDLE
                     SVELVRFVERHPDIEVAVVAAKDRSPMVPAHAAAPQPR"
     gene            complement(12256..13494)
                     /gene="serB"
                     /locus_tag="PLB86_05795"
     CDS             complement(12256..13494)
                     /gene="serB"
                     /locus_tag="PLB86_05795"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRC64682.1"
                     /translation="MTGASPHTLRVTLSGDDRPGVTFALLAAVAEAQAEVLDLEQMVV
                     RDHLTLSVLLGGADTDRLTSLAHSVGHRLGMRTRTRVGQGDSNTRRDGRAHVVALGQP
                     LTASGLAAVTARIAAHGANIDRIRRLSRYPVTTIELDVSGADVPRLRVELATVAAAER
                     IDIAVAPAGLARIGSRLVVMDVDSTFIQQEVIELIAAHAGCEEQVAAVTERAMRGELD
                     FAESLRARVALLAGLPATVLDEVRDEIVLTPGARTLVRTLKRLGDTVALVSGGFLEIV
                     GPVAADLGIDHVLANRLEVADGRLTGRVDGEIVDRPGKAAALRRFAAEAGLPLSRTVA
                     VGDGANDLDMLAAAGLGIAFNAKPLVREQADASVNVPYLDAVLFLLGITREDVEDADA
                     DAGTPTPAPPVISPGNPTRV"
     gene            complement(13505..14077)
                     /locus_tag="PLB86_05800"
     CDS             complement(13505..14077)
                     /locus_tag="PLB86_05800"
                     /inference="COORDINATES: protein motif:HMM:NF022167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HRC64683.1"
                     /translation="MAVSDIEAVEILDLLESVGAQPWVAGGWGVDALMERQTRMHRDL
                     DVMVLAKRLDDALAALRANGFQVTVDWLPVRVELDDGLRVVDVHPVHPDGSGGWWQAA
                     PDDGRYLYPAGSIVGGLIGGHTVRCASPALQRQVHTGYPLRHVDEHDIALLDEMLADA
                     DADADDLEAAGNAAQGPGPWAAGRGIPETL"
     gene            complement(14079..14984)
                     /locus_tag="PLB86_05805"
     CDS             complement(14079..14984)
                     /locus_tag="PLB86_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRC64684.1"
                     /translation="MSFQPQGQQRDGIPASAGDPRPARVAVQIAPQHAPYAVLRDTCV
                     AMEELGVDVVLTWDHFFPLSGDPDGMHFEAWTLLAAWAEATERVQLGVLVSAMSYRNP
                     NLLADMARTVDHISAGRLILGIGAGWFERDYVDYGFDFGTPGSRLDALARDLPVLKDR
                     WTRLNPLPTRAIPVLVGGGGERKTLRITAEHADIWHGFGDPATIRHKHLVLDEWCDRV
                     GRDPLAIERSAGVAYTPARLPEDATLDFAGQAQALFEVGTRLFTISLTTPPYDLGPIR
                     EVLAWRDAVNAPRAADQGSPTSLPV"
     gene            complement(15060..>15472)
                     /locus_tag="PLB86_05810"
     CDS             complement(15060..>15472)
                     /locus_tag="PLB86_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRC64685.1"
                     /translation="ISVHPIFNLYNYDWPIYTSMAPLPPAKFVHGSGDRVGEALSSMV
                     SPGVVVSGAQVNHSVLSPKVRVHSYATIADSVLLDDVEVGRYAVIRRAIIDKGVYIPE
                     YCRIGVDHEHDRARGFYVTEGGITVIGKGQKVPL"
BASE COUNT         2369 a         5286 c         5565 g         2252 t
ORIGIN      
        1 cccgacgcca ccgaggagca gatgcgggcg gcgctggagg ccgcccaggt gctgcacctg
       61 gtcgattcgc tgccgtccgg cctggacacc gtggtcggcg atcgtggcca ccggctctcc
      121 ggcggcgaga agcagcgtct ggccatcgct cgcctgctgc tcaaggcgcc ccacgtggtc
      181 gtcctcgacg aggcgaccgc gcacctggac agcgagtcgg aggtggcggt ccaacgcgct
      241 ctcgatgtgg cgttggccgg tcgcacggca ctggtgatcg cccaccgact gtcgacggtg
      301 cggggcgccg atcagatcct cgtcgtcgag gacggacgga tcgtcgaacg gggcaagcac
      361 gcggagttgc tcggtcgcgg cggcctgtat gccgacctct accgcacgca gtatttcgag
      421 gacagcacga ccaaggccgg tccctggtga cctgacggcg acgtcagccg tcagccatcc
      481 tcttcgtccc agatgcgcac cttcttgggc tcgggcgcct cggtgggggt ggcgttcggg
      541 acaccgccgg gtggatgccg atagaggtag accacgaagc cgaaccccaa cagcgtcgtc
      601 agccaccact gcagggcgta ggcgaagtgc ggcccgcggt cgggggtcgg cgcctccaac
      661 ggtgtggggc gagcgggggc cggcagtccc tcacccagcg actcgctccc cagcacgagg
      721 tatgccgcgt agagctgacc aggcacctgc tcctgggcct gggcgaggtt gatcgtcgcc
      781 agctgaccgg cgggcaggtc cttgccgcgg tcctcctcgc cgggcttgag ccagccggtg
      841 agcgtcactg ttccggccgg aggtgccgac accgtcggga ggtccgctgc ctggtcaccg
      901 ttgggcaccc acccacgatc gaccagcagg accgcgccgc cgtcgacctg gaaagggacg
      961 aggatctcca tgccttgggc ggtgttgagc gcccgtccgc ggacgatgag gtgggtgctc
     1021 gcggcatacc ggccggtgag cgtcacctgc gtccacacct ggctctcggc gagcggggtc
     1081 gtgaccgacg ggagcacgcc tgcgaagggg actgcaggcc ggttgtagtt ctgctcgacc
     1141 gccgtggcgc gggcgtcctt ctcttcgtag cgggacaact gccactggcc cagcaggaag
     1201 cacacggtgc cgaagccgag ggcgaacgcg acggcataca gaatccgccg ggtgaagatc
     1261 gcacgccaca cccgttcacc gtaaccgacg taaggtgacg cctgtgacgc ggctgcccga
     1321 cccccgcccc cgacccccgg agggatcggc gctgatcgac acctacgggc ggatcgcgca
     1381 ggacctgcgg gtctcgctca ccgaccggtg ctccttccgg tgcacctatt gcatgccggc
     1441 cgaggggctg ccgtggatcc cgcagccgca gatgctcagc gacgacgagt tgatgcggct
     1501 catcggggtc ttcgtcgggg cgggcgtcac ccaggtccgc ctcaccggcg gcgaaccatt
     1561 gctgcgtcgc agcatcgtcg agttggtcgc ggggatcgcc gccctgcggc cgagacccgc
     1621 gatcgcgatg acgaccaacg gggccgccct ggccgccgtc gccgcgccgc tggccgccgc
     1681 ggggctcgac cgggtcaacg tgtccctcga caccatcgac gcgagtcagt tcttcgcctt
     1741 gacccgtcgc gaccggttgg ccgacgtcat cgccggcctg gccgcggccg ccgacgccgg
     1801 gctgaccccg gtcaaggtca atgccgtcgc gatgcgtggc gtcaatgacg ccgtcgtcgc
     1861 cgatctgttg gcctggtgcc tggcccgggg gtatgagctg cgcttcatcg agcagatgcc
     1921 tctggacgcc caacacggct gggatcgctc ggtcatggtg acggccgagg agatcaaggc
     1981 cggcctggag gcgcgtttcg agctcacccc ggtcgaatcg accgatcggg ggagtgctcc
     2041 cgcggcgcgg ttcctcgttg acggcgggcc cgcgacggtc gggatcatcg ccagcgtctc
     2101 cgagcccttc tgttccgcgt gtgaccgggt gcgcctcacg gccgacggtc aggtccgcaa
     2161 ctgcctgttc tccgagcgcg agaccgacct gcgtgggccg ttgcgcgacg gggcgagtga
     2221 cgcggagttg ctcgggctga tcaggggcga gatgtggcgc aagggcccgg cccatgggat
     2281 cggcaggccc gacttccacc agccggatcg gccgatgtca gccatcggtg ggtgagtcgt
     2341 ccccctcggc gtcaccgacg aaacggatcg gcgcccaaca ccggctcgcc ggtcggcgtc
     2401 gacgccgaca actccgggtg ggtgggctcg tacttgtcga tgccggagat ccgggcgccc
     2461 cacctgggcc cgaccgcgtt ggccaggacg acagcgatgt agggcaggac tgcggcgccc
     2521 gcgacgaaca cccaccgcag ccacccctcg aagacgacgg ccaggacgaa gcacaggacc
     2581 cggatcgcca tcgtgatgag gtagcggcgc atccgcatgt cagtgtcctg cgcaagcgac
     2641 atcggcgcgg aagtcaccgt gtggaccacc gtgggcgtct gcggcttacg tccgagcctc
     2701 acctctcaac catacgactc ggtatgccgt gccgtcgcct tccgctcggc cccgcaccgg
     2761 gcgcgagcca cgcgtcttac ggcacgtccg tgcgggtcta cccaggggta accgactacc
     2821 gttcctcgca cacgtcgacc aacacccgag gagcctcacg atgaccgacg cagcgccggg
     2881 gcgcaccgtt ctggtgacag gcggtaaccg cggcatcggg ctggccatcg cccgctcgtt
     2941 cgccgaggcc ggagatcggg tcgtcgtcac cacgaggtcg ggggaggccc ctgatgggct
     3001 ggacgcggtg ccctgcgagg tgaccgactc cgcgtcggtc gacgcagcct tcgccgctgc
     3061 cgaaggcatc ttcggcagcc ccgttgaagt gctggtcgcc aacgccggag tgacgcgtga
     3121 ccaactgctg ctgcggatgt ccgacgagga tttcgactcg gtcatcgaca ccaacctggc
     3181 gggggccttc cgctgtgccc gcagggccag caagggcatg atccggctca agcgtggccg
     3241 gatgatcttc atctccagcg tcgtcggcct ctacggctcg cctgggcaga ccaactacgc
     3301 ggccagcaag gccggcctcg tcggcatggc ccgttcgatc agccgagagc tcggcgggcg
     3361 caacatcacg gccaacgtcg tcgcacccgg attcatcgac accgacatga cggcggcgct
     3421 gcccgacgag cggcgcgcgg catacctggc gagcatcccg gccggacgct tcgccgcgcc
     3481 cgaggaagtc gccgccgtgg tgcgcttcct ggccagcccc gaggcgggct acgtcacggg
     3541 ggcggtcatc cccgtcgacg gcggcctcgg catgggccac tgaccacctg ccacctgtcc
     3601 tgacttgacc tgacttgact tgacccgatc taacctgacg tgacccgacc tgaaagcgag
     3661 ccagcccgtg ggcatgttcg acggtaagac cctgctcatc accggtgtcc tcatggacac
     3721 ctcaatcgcc ttccacgtcg ccaagttggc ccaggagcag ggggcctcgg tcatcctcac
     3781 ctccttcggg cggacgatga agatcaccca gaccatcgcg cgccggctcc cggtgacgcc
     3841 gccggtcgtc gagctcgacg tcagcaaccc ggagcacctc gacaccctcg ccgagcggct
     3901 acgcgagcac accgaccgcc tggacggcgt gctgcactcg atcggcttcg cgccgttggg
     3961 cgcgttcacg ttcctcgacg cgacgtggga ggacgtcggc acggcggtcc acgtgtcggc
     4021 ctacagcctc aaggcgctgg ccgtggcggc gctgccgctc atggagccgg gcggctcgat
     4081 cgtcgggctg accttcgacg cgcgctacgc ctggccggtc tacgactgga tgggcgtggc
     4141 caaggcggcc ttcgagtcga ccaaccgcta ctgcgcccgc gacctcgggc ccaagggcat
     4201 ccgctgcaac ctcgtcgcag cgggcccgat ccgcaccacg gccgccaagt ccatccccgg
     4261 cttcgaggtc ttcgaggagc agtgggacac ccgcgcgccc ctcggctggg acgtcaccga
     4321 cccgctgccc acggctaagg cctgcgtggc gctgctgtcg gactggttcc cggcgacgac
     4381 cggcgaaatc gtccacgtcg acggcggcgt ccacgccatg ggctgctgag cgttcccgtc
     4441 gatcggtcgg gggaactccg ttgcaccact aggctcgcgc gacatggagc cagtgcacgc
     4501 cgatgaagtt cggatcctcg aaggcccgaa cctttatgtc acccggccgg ccgtcaaggt
     4561 cagcctcgcg ctgccgggcg tcgctcaact cgacgtccac gcggccctgg atctggcgcg
     4621 tcgcgtcgga ctgcgcacgg cccgccccgg gcgtcccgac accgggcagc gccagcgctt
     4681 cctgatgcgg ctcgtcgagc acctcgtgcg ccgcgtggcg accgagtcgg gcgcgcgacg
     4741 actggccgtg cggacccggg ccggacggca taccgaagac gtcgtggtcg ccgtgccgtg
     4801 gaggcaccgt ggccgagcga tcgcgttggg ggagagcctc gccccgctgc tgagcgacct
     4861 gctcaaagca gacgccgaca ccgaagcgct catcgctgcc gccggtcgcg ccgtcgccga
     4921 ggcccctgat ggagagcccg ctgcggtgct cacgccgacg gtcccggtca tcgccattac
     4981 cggcacgaac ggcaagacga caacgacacg gctcgtcgcg cacatggtca tgacggccgg
     5041 tctgcgcacc gcgtggagct cgaccgatgg cgtcgtcgtc atgggcgaga tggtgcagcc
     5101 gggggactac tccgggccgg gtggggcgcg cgaggttctc aacacgccgg ggttgcagtt
     5161 gggcatcctt gagacggctc gcggcgggat gctcctgcgc gggatgggtg tcaaccagac
     5221 cgacgtcacc gttgtcacga acgtcgcggc agatcacttg gggctcaacg ggatcgacac
     5281 cctcgaccag ctcgctgagg tcaaggccat cccgaccctc atcgtcaaac ccgacggctg
     5341 gacggtgctc aacggggatg acccgcgggt gtgggccatg cgccaccggg cgaagggcaa
     5401 gatctgggcc ttctcgctcg atcccaatgc cccggcgctg cgggaggcgg tggcgggcgg
     5461 cggccgcggg atcaccgtcc tggatggcga gatcgtcgtc ctgacccctg ggcgtgaccc
     5521 cgatcgtctc gtcaaggtcg tcgacgtccc catgaccctc gcgggcctgt ccagcatcaa
     5581 catcgccaac gcgctcggtg ggaccgccgc cgggttgggc ctgggcctga cccgggccgc
     5641 ggtcgtcgag ggcctgcgga ccttccgccc cgaccccgtc ctcaactccg gccggttcaa
     5701 cacctactcc gttcccctca ccggcggtgg gtccgcgacg gtggtcatcg acatggccca
     5761 caacgaggcg gggctggaag ccctcctgga ggtatgccgc gggctcaccg ccccgggtgg
     5821 gcgggtgcgg ttggggatcg gtggcacggg ggatcggctg cccgaggcca acgcggccct
     5881 cggcgagatc gccggccgcg gagccgatca cgtggtcctg gcgcacaagc tgaaatacct
     5941 gcgggggatg agcctggagc agatggactc gttgctgcgc gccgggctgg cccgatcagg
     6001 catggcggag gtcgcgggct tcctcaccga gatcgaggcc ttgaccaccc tgttggccgc
     6061 ggccgagagc ggtgacgtca tcgggctgat gtgtcacgtc cagcgggccg aggtcgccga
     6121 cctcattgcg cagcggggtg gcaccgtgga cgacccgcgg acgatccgca gcaaggtcat
     6181 cgcagcgcgc ggcatgcacg aggccgagga cgacatcgcc gcgatctggg ccctcaccga
     6241 tgccggcgag cgggtcacgg ctgccggtcg gctggcgaca gcccacccgt cggacccacg
     6301 gctggcctac gagtgggcct gtgcgatcga tgccaccgca gaccgggccc gggcggtctc
     6361 gctctatcac cgcgccctgg acaacggcct gcgcgagcca caccgccatc gcgcccagtt
     6421 gcaggcggcc gcggcatacc gcgatctcgg gcatccggag atcgccctgg agttgctcga
     6481 agcggtcgca gcgactcacc cgggcagcag tgtcgccgcg gcgctgcgcg cgctggtgct
     6541 cgtggacacc ggggacgcgg cggaggcggt tgctgacctg gtggacgccc tcatggagca
     6601 cgccacggat gaggacgccg cggcataccg gacgttcttg caccacgccg ccgcccagtt
     6661 gcgcaccgac cgacccggtg ttgtggactg aggaactcga cggggcaact cgggtggccc
     6721 tgagcggctg ctaagccgcg ctgcgctcag tgaagtccca tgctgaggac caggcccgct
     6781 gcggcgaggg cgagcactgc tcccgcccag ctgagcgctg cgagccgggg acgatgccag
     6841 ggccgggtgc ggacccgcgc gatcccgagc atcacggtcg ctgcggtggc ggccgcgacg
     6901 aggatccaca gcagccccgg agcgtggtcc catggcccgg cgacccgcca gagggagagc
     6961 ccgaggagcg cgcccgcggt ggctcgcacc agttgcaggc gggtccgtgc ccggtatgcc
     7021 gcgaagccgg ctcccgcgat caggaccagc gccaccgtgg tggcggccac agcgatcacg
     7081 ggcatcgggg cgtcagtcga accgggtccg tcggcgacgc cggtgagcag tcggatggcc
     7141 agtgggtcga cgggccgcgt gctgttgcca acaacgaaga ctgcgcgacc cgccaagcgg
     7201 tcgatgacga gcatggaggt atagccagcc gtggcgccgt tgtgccactg gacggtgcgt
     7261 ccggccaccg tcagcgtgaa ccagtggtag ccgaccctcg ggttgccggg gctagcggcc
     7321 tgctcgccgg cgaaccgagg atcgagggcg ggcaggccag gcgcggtgcc ccgcaggatg
     7381 gcttgggcgt agcgggcgat gtcttcgggc gtggtccacg tgctggatcc ggcgggctgg
     7441 tagacggggc tgactccctg gacggcctgg cggccaccga ccaaccggcc ccgaggtgcg
     7501 tcgggcggga tctggtcacg ggtcgccgcg aagcgggtgt gggtcatggc cagcggcgtc
     7561 agcagccggg tcgtgacata ggtctgccag tccggctgct gggcggctga tgccagggcc
     7621 cacccgagca gggtcgcgcc gagattggag tactggaccg tgccgcgctc gccgaagagc
     7681 ggcatcgcgg ccgcctggtc gagcacccag tcgagttggt cgccggacag ttggtcggtg
     7741 ttggagagca acccccaaag cccacgagcc atggtcggcg gtggaaaggg cggaacggcg
     7801 ccgcggtggc tggccagttc ctccagggtg acccctccga tcggactgcc agccaggcga
     7861 ggcaggtagg tcgagaccgg gtcggttgcg gcgacttctc ctcgggcgat ggcatcggcc
     7921 aacagcagtc cgttgaaggt cttggtgatg gacccgagct cgatgggcgt ggcagcgcct
     7981 agtgcctccg cgccccccgg ttccatcgag cccaccccgg tatgccgcac cgaggttggc
     8041 gtgatctcgc tgacgtgcag ggcctggtag ccgcggtcgt cctccaccgc ggcacgcacc
     8101 ctggcagcga ggtcgaggtc gccgctggtg gccggacctc cgcggagcga gaggggggcg
     8161 accaaggcag ccagcaggcc cgcgatcacg aggaccgaga caagggccgc aggccggggt
     8221 cgacgggcgc gacgccctgc gaccccggcc gccggtgcta cgtcaggggc ctggggcccg
     8281 ggctgaggct gcggtttcac cgcaccgatc atcggcgtcg ggggagtggc gtccgtcatg
     8341 tccatcaatc tacgttgatg gacatgatgg cgtcaacttt ggttgatgcc ctcccggcta
     8401 ggcgttgggt gcgcctgttg tttcgggaac cggcgtggac agggcccggc cttcggcagc
     8461 ggccccagac acggcggcgg caccggtgag gaccaccggg tcgaagtcgg ggtccgagac
     8521 gccgccgggg tgagcccccg gctgggcctg gcgggtcatg tcctcgaggg ccgcgaacac
     8581 ctcggccgag ttgtcgacgc agacaacgac gaggtcgcca gggttggccc gagccatgac
     8641 atcgccgacc gcctgcaact cgtccaggac gatgtcgacc tgccggcagc ggacgccctt
     8701 ctgcatcgct tccttgaccc cttcgcggat caactgggct gcctcgccag gacggcgacc
     8761 gcgaaggcgc gcgtcctcac ggatgaccag cacgtcgaag tgctccgccg cgacggcacc
     8821 caactcgcgc agatccgagt cgcgccggtc accggcggca ccgatcgccc cgatgcgcga
     8881 aacccgaccg aggtcggcgc gaccggcgcg agtggcggca tacctgtcga cgaagtcgcc
     8941 caacgcccgc atcccagcag cgttgtggca gtagtcgacg aagacctcga cgttgttgac
     9001 ctcgacgagg ttgaggcgcc cgggggagag gtagtagttg ctggtgaacg tccgaaggcc
     9061 ctgccggatg tcgtgcagcg gcgcgcctgc ggcgaaggcc gcaccggccg ccgcgagggc
     9121 gttggcgacg ttgaagcggg cggtgccgcc gaaggtcgcc ggcaacaggt gggtccaggc
     9181 gagttgcata gcgcgccgcc cgtgttggat gacgatcatg tcgccgcgat cggtcgggtc
     9241 gagatagagg gcccgacccc cgcgccgaca gtgggcgtcg acgaactcgc ggatgtccga
     9301 gccgggggcg gccagggtga accagacgat ctcgccggag cacttgcggc gcatcgaccg
     9361 cacgtggggg tcgtcggcgt tgaggacggc gaagccgttc ttggggactg cctcgacgac
     9421 gacagccttg acggcggcca gttgggcgag agtgtcgatc ccgcgcagac ccaggtggtc
     9481 gggggccacg ttggtgacga cggcgacgtc gttgcggtcg taccccagac cctcacggag
     9541 tatgccgccg cgcgccacct ccatgacggc gaagtcgacg cgggggttca tgagcaccat
     9601 ccgggccgac ttcgggccgg atgcgtccgc cttgatgacc agccgctcgt cgatgacgac
     9661 gccgtcggtg gaggtcatgc cgaccttgcg acccaagccc ttgaagatgt gggcgatcat
     9721 ccgcgaggtg gtcgtcttgc cgttggtgcc cgtgacggcg acgatcggca cgcgggaggg
     9781 ggctcccggc gggaagagca ggtcgacgac cggcttggcg atgaactgcg gcacgccgac
     9841 cgtcgggtgc gtgtgcatcc ggaagccggg cgcggcgttg acctcgacga tggccccgcc
     9901 ggtctcgcgg acaggagccg cgatgtcagg gcagatgaag tcgatgccgg caacgtcgag
     9961 gccaaccatt cgggcagcct cttcggcgat ctcgacgttg tccgggtggg cgtcgaaggt
    10021 gcggtcaacc gagatgccac cggtggacat gttgccggtg agggcgagtt tgaccatgac
    10081 accggcatcc gggacgtcgg tgaggaggta gccctggcct tcgaggacct cgatggcggc
    10141 ggcgtccacc ttgatccggg tgaggacctt ctcgtggccg acaccacggc gcgggtcgga
    10201 gttggtgatg tcgacgagtt ccttgacggt gtgctcgccg tcgccgacca cgtgggcggg
    10261 gacgcgctcg gcgatggcct gcatccggcc gccgacgatg aggcagcgat agtcgcgacc
    10321 ggtgacgaac gactcgacga tgaggacgcc gctgcgggac tgctcctggg cgatgtcgta
    10381 ggccttgtcg acctcgtcga gggtctgcaa gtccagacag acgccgcggc cgtggttgcc
    10441 gtccagcggc ttgaccacga cggggaagcc gatctgacgg gcggcgtcaa gcactccacg
    10501 ccgggtgcgc acggcttgct gcttgggagt cggcagaccg gcggccgaca gcaacttgcc
    10561 ggtgaggtcc ttgtcgctgg cgatgtccac ggccagcgcg ccggtgcgcg aggtcatcgt
    10621 cgcgcggatg cgctgcgcgt gcaccccttg accaagctgg acgagcgacg ccgagttgag
    10681 gcgcagccac ggaatgtcgc ggctgacggc ctcctcgatg atcgcggcgg tcgacgggcc
    10741 gaaggcgacc cggtcggccc gacgcaagaa cgcgtccaac tgctcggtga agtcgaagcc
    10801 gtccgtgggc tgcacgaggt ggttgatcag ccgcaccgcc aactcacccg cggcgatgcc
    10861 gaccgtctcg tcgacgtagc cgaagatgac gttgtagacc cccggccgac ccttgaccat
    10921 ccgcgtcttg ccacgacgca ggtcgtggcc ggcggtggac tgcaattcga gcgcgacgtg
    10981 ctcggcgacg tggccgagcc aggtcccgtc ctcgagccgc tcaatgaagc cgcccgcatg
    11041 acccaccgag caggtatgcc gttgcagtcc aggcagcagg gccacgagcg cgtcggtgaa
    11101 gccgtcgatc gagtcgctcg gatagccctc gaggacaccg agatcgacga cgagatgaat
    11161 ggctggcgca taggaccaga cattgggacc ccgatagacg cgcgtctcga ggatgcgcag
    11221 atccggcgcg atcgggccgg tcggggtggg gtggtccttc gccatgcgag ctccttgccg
    11281 cggagtgggg aggatcacac gctagtggtc agcgtggttg tggcgcagca gcgtgcgcgg
    11341 gcaccatggg cgagcggtcc ttcgcggcga cgacggcgac ctcgatgtcg gggtgtcgct
    11401 cgacgaagcg aaccagttcc accgactcaa gatcgaaggt ggcgccggcc ggcagggtgt
    11461 gaacgacggc ccccgacacc agcaggggag cgccacgtcg ggcctcaggg gcgtcggtga
    11521 tggcatgccg cgcgtcgagg acgaagaccg ctccagcacc gacgaccgac atcaggcgtt
    11581 cgtcacggat ctcggcggcg gtgttctcgt cgatgccgat cccgatcaga ttgggggagt
    11641 tcgcgacgat cgacagcagc cgcccgtagc ggccgcgttg atcgaaatgc tggtcgacca
    11701 cgacaccggt gaccaggccc aacccggccg agagttgact cgaccgttgg cgcggcgtca
    11761 cgccctcgtc gccgagggag atcatgaact ggctcatgat cgaggctccc gcagaggttc
    11821 cggcgatgac cgccccccgc tggtaggccc ggtggatcgc atcgccgacg ggcgtgccga
    11881 cgatgttctg gctgagcttg agttggctcc cgcccgtcat gaagatcccg gtcgccgcgt
    11941 ccagtcgggc caccaactcc gcggacgagg cggcagcccg ggtcggcggg tggacgatct
    12001 cgaagtcctg gccagccttc agcctggcaa agacgctccg atagagcgcc gacgcctcgg
    12061 tcgagaagga cgatgcggtc gggatgacga cgatccgcga cgcgcgcccg cctgccagtc
    12121 ggacgaaccg gcgcaacacc accgagcgcc cgagcttgtc ctcggcgcct ccgacgatga
    12181 gcagggtgcg gcgcaccggc ggtgaggtgg gcggcatacc cgcctcgtcg gcaggacccg
    12241 cgttggaaac caccgtcaca ccctagtggg gttgccaggg gagatgacgg gaggcgcggg
    12301 tgtcggcgtg cctgcgtcgg cgtcggcgtc ttcgacgtcc tcgcgggtga tgccgagcag
    12361 gaagagcacg gcgtcgagat aggggacgtt gacgctggca tcggcctgct cacggaccag
    12421 cggcttggcg ttgaaggcga ttcccaagcc ggcggcagcg agcatgtcga ggtcgttggc
    12481 gccgtcaccg acggccaccg tgcgcgacaa gggcagcccg gcctcggccg cgaaccgccg
    12541 cagcgccgcc gccttgcccg gtcggtcgac gatctcgccg tcgacgcgcc cggtcaggcg
    12601 tccgtcggcg acctcgagcc ggttggccag gacgtggtcg atgcctaggt ctgcagcgac
    12661 cggcccgacg atctcgagga agccaccgga caccagggcg acggtgtcgc cgagccgttt
    12721 gagggtgcgc accaaggtcc gcgctccggg ggtgaggacg atctcgtccc ggacctcgtc
    12781 gaggaccgtc gccggcagcc cggcgaggag cgcgacccgg gcccgcagcg attcggcgaa
    12841 gtcgagttcc ccccgcatgg cgcgctcggt gacggcggcg acctgctcct cacacccagc
    12901 gtgggctgcg atgagctcga tgacctcctg ctggatgaag gtcgagtcga catccatgac
    12961 gaccaggcgg gagccgatcc gcgccagccc ggcgggggcg acagcgatgt cgatgcgctc
    13021 ggcagcggcg accgtggcca actcgacccg caggcggggg acatcggcgc cagacacgtc
    13081 gagctcgatg gtcgtcacgg gatagcgcga caagcgccgg atccggtcaa tattggcccc
    13141 gtgcgcggcg attcgcgcag tgacggctgc caggccgctg gcggtcaggg gctgcccgag
    13201 agccacgacg tgggctcgcc cgtcgcggcg cgtgttggag tcgccctgtc ccacccgggt
    13261 gcgggtgcgc atcccgagcc ggtgcccgac ggagtgggcc aggctggtga ggcggtcggt
    13321 gtcggcgccg ccgaggagca ccgagagggt gaggtggtca cgcacgacca tctgctccag
    13381 gtcgaggacc tcggcctggg cctccgccac ggccgccagc agcgcgaagg tcacccccgg
    13441 tcggtcgtcg cccgagaggg tcactcgcag ggtgtggggg gatgcgccag tcacgcttcc
    13501 caggctagag cgtctcgggt atgccgcgtc cggccgccca cgggcccggc ccctgcgcag
    13561 cgttccccgc ggcctcgagg tcgtcggcgt cggcgtcggc gtcggccagc atctcgtcga
    13621 ggagggcgat gtcgtgctcg tcgacgtgcc gcaacggata gccggtgtgg acctggcgct
    13681 gcagcgcggg ggacgcgcat cgcacggtgt gtccgccgat gaggccaccg acgatcgacc
    13741 cggccggata gaggtagcgc ccatcgtcgg gcgcggcctg ccaccagccg cccgacccat
    13801 cggggtggac ggggtgcaca tcaacgaccc gcagtccgtc gtcgagctcg acgcgcaccg
    13861 gtaaccagtc cacggtcacc tggaaaccgt tggcgcgcaa cgcagccagc gcatcgtcca
    13921 gtcgcttggc cagcaccatg acgtcgaggt cgcgatgcat ccgggtctgc cgctccatca
    13981 gggcgtcgac accccacccg cccgccaccc agggctgcgc cccgaccgac tcaagcaggt
    14041 cgaggatctc gacggcctcg atgtcagaga cggccatgtc agaccggcaa ggaagtaggc
    14101 gacccctgat cggcggcccg tggcgcgttg accgcatccc gccaggccaa cacctcgcgg
    14161 atcggaccga ggtcataagg gggagtggtg aggctgatgg tgaacaaccg ggtcccgacc
    14221 tcgaagagcg cctgcgcctg gccggcgaag tcgagcgtcg cgtcctcggg cagccgcgcg
    14281 ggtgtgtagg cgacccccgc ggagcgttcg atggccagcg ggtcccgccc cacccggtcg
    14341 caccattcgt cgagcaccag gtgcttgtgc cggatcgtcg ccgggtctcc gaaaccgtgc
    14401 cagatgtcgg cgtgctcggc cgtgatccgc agggtcttgc gttcgccgcc gccacccacc
    14461 aacaccggaa tcgcccgggt gggaagggga ttgagccggg tccaacgatc cttgagcacc
    14521 ggcagatcgc gggccagggc atccagccgc gacccgggcg tcccgaagtc gaagccgtaa
    14581 tccacgtagt cccgctcgaa ccagccggca ccgatgccga ggatcagtcg gcccgcgctg
    14641 atgtggtcga cggtccgcgc catgtcggcg agcaggttcg ggttgcgata ggacatcgcc
    14701 gacacgagca cgcccaactg cacgcgctcg gtcgcctcgg cccacgcggc cagcagcgtc
    14761 cacgcctcga agtgcatccc gtcggggtcg cccgacagcg ggaagaagtg gtcccacgtg
    14821 aggacgacgt cgacgcccaa ctcctccatc gccacgcagg tgtcgcgcag cacggcatac
    14881 ggagcatgtt gaggagcgat ctggacggcg acgcgcgcag ggcgcggatc acccgccgat
    14941 gccggtatgc cgtcgcgctg ctgcccttgc ggttggaagc tcacccacgc aggctagccg
    15001 ttcgcggcaa tcccgctcag ggcggcatac ccgctggttg ccatcagttg ccgccagcct
    15061 cagagcggga ccttctggcc cttcccgatg accgtgatgc cgccctcggt gacatagaag
    15121 ccgcgggccc ggtcgtgttc gtgatcgaca ccgatccggc agtactccgg gatgtagacg
    15181 cccttgtcga tgatggcgcg gcggatgacg gcataccgtc cgacctccac gtcgtcgagc
    15241 aggacgctgt cggcgatcgt cgcgtaactg tggacgcgga ccttcggcga gagcaccgag
    15301 tggttgacct gggcaccact gacgaccacg ccgggggaga ccatcgagga caacgcctca
    15361 cccacgcggt caccggagcc gtggacgaac ttcgccggcg gcagcggggc catcgaggtg
    15421 tagatcggcc agtcgtagtt gtagaggttg aagatcgggt ggaccgagat ga
//
LOCUS       DAORPW010000047        15469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_750_length_15469_cov_854.637667, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000047 DAORPW010000000
VERSION     DAORPW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15469
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_750_length_15469_cov_854.637667"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..869
                     /locus_tag="PLB86_05815"
     CDS             231..869
                     /locus_tag="PLB86_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64686.1"
                     /translation="MTVDDHPDRPAARSAGRPRRPLYLDSAGLEAQAGSFDPVQEAQA
                     AHESAAILVHRGRASDDPQLTARLVALAEEVGLDTLAHLWATRPARSLPGALWRLYVL
                     RDWVRRDPAGASREYAAGIRYAAVNHVVAGPADPPGPEELRTLLDAVLRGVFEGDLAV
                     ALERAAAFARVVSAGRAAESEGEAAATQAADLLATAEDLTASARAWRQGALA"
     gene            1120..1266
                     /locus_tag="PLB86_05820"
     CDS             1120..1266
                     /locus_tag="PLB86_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64687.1"
                     /translation="MSGSSAQGEIHDMAKLEEMELVDNLEMAAAAAPQRRLTAEEIDQ
                     ALGL"
     gene            1351..1536
                     /locus_tag="PLB86_05825"
     CDS             1351..1536
                     /locus_tag="PLB86_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64688.1"
                     /translation="MTEEQHDHEVTTPYSETLRAVAESEPLTWPADDATVTETGDELA
                     SLAEDQLKAHMVVGRMY"
     gene            complement(1584..2360)
                     /gene="pstB"
                     /locus_tag="PLB86_05830"
     CDS             complement(1584..2360)
                     /gene="pstB"
                     /locus_tag="PLB86_05830"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRC64689.1"
                     /translation="MANRLKVEDVNIYYGAFKAVEGVTLTFEPRSVTAFIGPSGCGKS
                     TVLRTLNRMHEVIPGGRVEGSITLAGQDLYAAEVDPVAVRRTVGMVFQRPNPFPTMTI
                     FDNVVAGLRLNGLRNKSELADAAERSLRGANLWNEVKDRLNKPGAGLSGGQQQRLCIA
                     RAIAVEPQVLLMDEPCSALDPISTQAIEDLIEELKTDYMIVIVTHNLQQAARVSDQTA
                     FFNLAATGQPGKLVEVGPTPRIFEHPVHESTEAYVSGRFG"
     gene            complement(2390..>2569)
                     /locus_tag="PLB86_05835"
     CDS             complement(2390..>2569)
                     /locus_tag="PLB86_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HRC64690.1"
                     /translation="NLFDGYMPTLPTMINQDRTEIGLDSAMERMWAAALTLILIVLAL
                     NLIGRWAARRSSVRR"
     assembly_gap    2570..2669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2670..3193)
                     /locus_tag="PLB86_05840"
     CDS             complement(<2670..3193)
                     /locus_tag="PLB86_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HRC64691.1"
                     /translation="MSATSAAETTEATGTPLARLVAARRAAYRADVVTDDPPQLTRGS
                     ASRRLTDQVARVVMWASFGLAMVPLVWILGTVVARGYELLLASSWWTLSQRNINAADV
                     GGGAVHAIQGTLIQAAVTAAISVPLGIFTAIYLVEYGRGRLARVVSFMVDILSGVPSI
                     VAALFVYAVWVTTFG"
     gene            complement(3190..4158)
                     /gene="pstC"
                     /locus_tag="PLB86_05845"
     CDS             complement(3190..4158)
                     /gene="pstC"
                     /locus_tag="PLB86_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRC64692.1"
                     /translation="MTEPAGSSWTATVTAPRRGGDRWFGLASQGAGVAVVALVTLVGV
                     FLVSQAIPALSKNEANFLTSREWSVGGDNPRFGIMELLWTTTTASIIAMAIAVPIGVC
                     VALFITEYAPQWLRRPAATLVDLLAAVPSIVYGLWGLIIFRPVVRPLEDWLSTRLGWL
                     PLFAPTGITGGTIMFIGVVLAIMILPIVTAISREVFAQTPTTHKEGALALGATRWEMI
                     RTAVLPFGKPGVISAAMLALGRALGETIAVTFIVSTLAAGSDWTWSMLSGGETFASRI
                     ANNAAEFDSPQKTGAFIAAGLVLFILTFVVNAVARVVIERRKAFTE"
     gene            complement(4213..5301)
                     /gene="pstS"
                     /locus_tag="PLB86_05850"
     CDS             complement(4213..5301)
                     /gene="pstS"
                     /locus_tag="PLB86_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HRC64693.1"
                     /translation="MTTRSSAIIIVAMSGALALGACGSDATKTGTAATGTAASDCPTG
                     TLKAEGSSAQKNAIEAAIKSFQTKCSGTTVNYAATGSGAGIKQFIAGQVDFGGSDSAL
                     KEDEAKSAETTCASPAWHLPMVTGPVGIAYNLPSVSSLVLNADVTAKIFLGQITMWND
                     PAIAALNSGTTLPATKISVFFRSDESGTTDNFTKYLKAAAGTAWTVESGKKWNGTVGE
                     GKPQNAGVATAAKSVEGGISYMEWSYVTQNKLGLAKIDNGSGPVELTSASVGKMIETA
                     TQKGTGNDLSLTLDYATKTPGAYPINLVTYEIVCSKYKDAAKGKLVKSFLTHFVSADV
                     QKSLEALGYAALPSSVSAKITAAVAAIG"
     assembly_gap    5352..5451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5452..6333)
                     /locus_tag="PLB86_05855"
     CDS             complement(<5452..6333)
                     /locus_tag="PLB86_05855"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRC64694.1"
                     /translation="MPTINAAGTVPWRVNGDALEVALVHRPRYDDWSWSKGKLEPGER
                     WPVAAFRETQEETGLQVRLGMPLPPTTYRVLDRDGVPATKWVRYWAAQVTGGDGSLTH
                     EIDEVAWLDATSADTRLDYSHDREQLRALLRAYQRGLLDTWPLLVVRHALATPRSAWK
                     GDDRKRPLSPAGHVQVPLITDTLAAYGVEALTTSPSTRCVQTLAGYAKRSGLDLHMRN
                     AISEEGFAKRGAKRVNRVVDEVLATGVPTAVCSHGPVLPTILEALVGRLAPVDELIPG
                     ADTSLAEAADLGIEKGEV"
     gene            complement(6343..8496)
                     /locus_tag="PLB86_05860"
     CDS             complement(6343..8496)
                     /locus_tag="PLB86_05860"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRC64695.1"
                     /translation="MPDSAAADEVEARPVVALDLPQERYLEREISWLQFNERVLQLAM
                     DETVPLIERAKFLAIFSSNLDEFFMVRVAGLKRRIATGLAVRSAAGLEPRELLDRIQH
                     AAQELMRMHSWVFWDQIRPALHEEGISIVRWGDLNEAERAPLHGLFREQIFPVLTPLA
                     VDPAHPFPYISGLSLNLAVVLVNPKTGAEHFARVKVPPVLPRFLRVFEPGDDHGTQRA
                     RFIPLEDVIAVHLDQLFTGMEVREHYTFRVTRNEDLEVEEDDAENLLTALEKELTRRR
                     FGPPVRLEVEDAIADRVLDLLTRELGVTEAETYRLPAPLDLRGLFLLGDLDRSELKDP
                     PFVPMTHPDLAPTERSQEGDLFAAIRSKDILLHHPYDSFSTSVQSFIGQAAADPRVLA
                     IKQTLYRTSGDSPIIDALIDAATAGKQVLAVVEIKARFDEVNNISWARKLEHAGVHVV
                     YGIVGLKTHAKLCLVVRQETEGLVRYCHVGTGNYNPKTARLYEDLGILTCDPQVGEDL
                     SRLFNQLSGIAPRSRFRRLLVAPRTVRSGLIELVDAEIERHEADGGGAIRLKMNSIVD
                     EQLIDALYRASQAGVPVDVWVRGICAIRPQVPGLSDNLRVRSTLGRFLEHSRIFWFAG
                     GGSPQVYIGSADMMHRNLDRRVEALVQIVDTSHVAELTNMFNMGLADTTSRFELGADG
                     TWTRVYRDAEGRPLNDLQTLMWDHHNKARRKARRR"
     gene            complement(8635..9546)
                     /gene="mshD"
                     /locus_tag="PLB86_05865"
                     /pseudo
     CDS             complement(8635..9546)
                     /gene="mshD"
                     /locus_tag="PLB86_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
     gene            complement(9575..10285)
                     /locus_tag="PLB86_05870"
     CDS             complement(9575..10285)
                     /locus_tag="PLB86_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC64696.1"
                     /translation="MAMLLLLTNTVAPSAEVLPALALLGHQVRILPAEPAALVHAPPG
                     DAVLVDARRDLAHAKSLCRVLRATGISTPLMAVLTEGGLAALNADWGLDDVLLDTAGP
                     AEVDARLRLAFTRVETEEAQEAGPIRAGDLTIDEVSYSARFRGRALDLTYKEFELLKF
                     LAQHPGRVFSRAQLLQEVWGYDYFGGTRTVDVHVRRLRAKLGPEHETLIGTVRNVGYR
                     FVPERPRDTIVAEVDAGV"
     gene            10404..10661
                     /locus_tag="PLB86_05875"
     CDS             10404..10661
                     /locus_tag="PLB86_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRC64697.1"
                     /translation="MPSGTVLLRYWAAARAAAGVDTEAVPVPVEGESVGPLLDAAVIR
                     HPELARVLKVASVLVDGRAADRDTRVAPAGVVEVLPPFAGG"
     gene            10678..11502
                     /locus_tag="PLB86_05880"
     CDS             10678..11502
                     /locus_tag="PLB86_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64698.1"
                     /translation="MSDPSAATSHRRGPHVGRFGDVDSTRLPGLVTVAALTTTGALAA
                     TSLAGPGLLAGLLGILALIVALGWPILSSPPALGQVQVGLAVGGLLIAATVGLTTGSH
                     RLVWLPVAVAIGMLVSFFLQLLRSDDRHGLTEAMSVVATGLACVGSGAALVPLILTEP
                     GVRFALIGLAGLAAGVLAELTGRMPRVGAKAVFVVMLAGGLGGWLAAVLLGSAGSAGL
                     GAGMLLASFSYSARRIFGAVPGSREAPGQVALGVATVLLPTVLLLALGALAKVYSG"
     gene            complement(11527..11910)
                     /locus_tag="PLB86_05885"
     CDS             complement(11527..11910)
                     /locus_tag="PLB86_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRC64699.1"
                     /translation="MARSLVVKLTALGDTWPPPTAELERVAQALTVATTALASGIPTS
                     VWLTGEATRLATPGYAARLTLPHAAPFEDLVDLLLDAEVVTVCTQCAARRGLTEADLL
                     PGARIAGAAGFVEQALAEGAQALVY"
     gene            11954..12454
                     /locus_tag="PLB86_05890"
     CDS             11954..12454
                     /locus_tag="PLB86_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HRC64700.1"
                     /translation="MFELDVNLPERLRPLAWLIGRWEGAGVLGYPTIESMHYGQEIRC
                     WHDGRAFLQWEARAWILDSENGERVRPASIETGFWRPGEDGREVELLLAHPTGILEMY
                     YGQMEPAKITLRTDSVMRSPHAKEYNAATRLYGLVEGDLMYTMDMAAVGEPMTPHLSA
                     QLKRVG"
     assembly_gap    12812..12911
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12960..13379
                     /locus_tag="PLB86_05895"
     CDS             12960..13379
                     /locus_tag="PLB86_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRC64701.1"
                     /translation="MSDLAAVLRANGMRLTPQRRRVVDALARLGHATPDAVAAEVGAD
                     GGSPLAASTIYRALEALEELGLVAHTHLDHRAPTFHLAGHADHIHLVCLGCHTIDEVP
                     VEVAAEFVAGMAAARGFRADITHMAVHGWCSSCGGPQ"
     gene            13376..14467
                     /locus_tag="PLB86_05900"
     CDS             13376..14467
                     /locus_tag="PLB86_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HRC64702.1"
                     /translation="MTGYAVPSPLLDAPGAVAAEGVDAGVAGHYGDPMREQRLLTEGL
                     AAVDLSNRGVVTVTGPDRLSWLNSMTTQALTGLTPGVGTESLVLDPHGHVEHALHLVD
                     DGTTTWITVEPGTAPALVAWLERMRFMLRVEVADVTDRYAVVGEPFGAVAAPGEPVVW
                     VDPWPGVIDLTPSGRAGRIAAYGPVDGHPAQGRRWREAIVPREELATYIDDRPLAGVL
                     AAEALRVAAWRPRLGFETDHRTLVHEVDWLRSAVHLNKGCYRGQEAVARVHNLGKPPR
                     RLVFLHLDGSGHVLPPRGAEVTVAGGGSGPLAGRAVGFVTSVAHHFETGPIALALVKR
                     SLPADAPLIVADGIGGPGIAASQEVIVAP"
     gene            14545..15399
                     /locus_tag="PLB86_05905"
     CDS             14545..15399
                     /locus_tag="PLB86_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRC64703.1"
                     /translation="MMSASSAAGTLITVAPTGAETAKADVPQLPTTLDELVETARRCE
                     AAGAGLIHIHVRDREHRPTLDAAYLRDAVVAVREATDLVVQLSTGGSVHDPLEKRLTV
                     LDADPDSCSLTCGTTNFGDDVFLNPWGFMVDLYRLAQEREVVPEFELFELGHVHALRR
                     LIDTCGLPFGGKVHVDFVTGVPGAMPGTVEALLAGVAMLPAEVTSWSATGIGRAHLPI
                     LGAALAAGGHLRVGMEDNLFVARGQVAQHNVEFVERAATLAGLMQRPALSTTQARELL
                     GIKDRRAR"
BASE COUNT         2251 a         5070 c         5536 g         2312 t
ORIGIN      
        1 cccacaacct acggattaaa agtccgcagc tctgccattg agctagagac ccggcagagg
       61 ccgacacccg ccggcccgaa gcatcagcca gcctaacgga ccggccccgt tacccgcgga
      121 ccgcgcgcgg gcggcatacc gtgaggggtc ggcaacccgc cccaccccaa ggggtatgcc
      181 gcgcagcccc cgtccgctgc cacccgccgc cgcgaccagg aaggaacgcc atgaccgtcg
      241 acgatcaccc ggaccgcccc gccgcccgca gcgcaggccg accccggcga ccgctctacc
      301 tggacagcgc gggcctcgaa gcgcaggccg gctcgttcga ccccgtccag gaggcgcagg
      361 cggcccacga gagtgccgcg atcctcgtgc atcgggggcg cgcatcggac gacccgcagc
      421 tcaccgcacg cctcgtggcg ctcgccgagg aggtcgggct ggacaccctc gcgcacctgt
      481 gggcgacgcg gcccgcgcgc agcctgccgg gcgcgctgtg gcggctctac gtgcttcgtg
      541 actgggtgcg ccgcgacccc gccggagcga gtcgtgagta tgccgcgggc atccggtatg
      601 ccgccgtcaa ccacgtcgtc gcggggccgg cggatccgcc tgggccagaa gaactgcgca
      661 ccctgctcga cgccgttctg cggggcgttt tcgaggggga tctcgcggtg gcgttggagc
      721 gggccgcggc gttcgcgcgg gtggtcagtg cgggccgggc cgcggagtcg gagggggagg
      781 ctgcggccac gcaggcggcc gacctgctgg cgacggcgga ggacctcacg gcgtcggcgc
      841 gggcctggcg tcagggtgcg ttggcctgac gggcgagaag ttaccgccca accgtcggtc
      901 gcgggtatcc tcggggtctg cgtcgggccg cggtagcccc gggtcccaca aatagccgct
      961 acgagcggcc ttgcgccgtg aggcgctccc ggcccgacgc acccacagca atcacacaac
     1021 cgtcgaccgt tcgtcgtcgg tgccagaccg tccggcgacg ccctcggcca acgatggcga
     1081 tcgccttcct gcgggcccgc cgttccctac tctgccccca tgagcggatc gagcgcccag
     1141 ggggaaattc acgacatggc caaactcgag gagatggagt tggtcgacaa cctggagatg
     1201 gcggccgctg ccgcgccgca gcgtcgcctg accgccgagg agatcgacca agccctcggc
     1261 ctgtgacctt tgtggcggat tgtcggtgcc ggggctactc tcgaaggacc gggcaccgtg
     1321 gcccgcacta ggggaaggaa cactgcgctc atgaccgagg agcagcacga ccacgaagtg
     1381 acaacgccct actcggagac gttgcgcgcc gtcgccgaga gcgagccctt gacctggccc
     1441 gcagacgatg cgacggtcac cgagaccggt gacgagctcg cgtcgctcgc cgaggaccag
     1501 ctcaaggccc acatggtcgt cggccggatg tactgacgag gccggatgct cgccccaggc
     1561 agacctgggg cgagcatgat ctgtcagccg aagcggccag agacgtaggc ctcggtcgac
     1621 tcgtgcacgg ggtgctcgaa gatccgcggc gtcggaccga cctcgaccag cttcccgggc
     1681 tgaccggttg ccgccaggtt gaagaacgcc gtctgatccg acacccgagc agcctgctgc
     1741 aggttgtgcg tgacgatgac gatcatgtag tcggtcttga gttcctcgat gaggtcctcg
     1801 atggcctgcg tcgagatcgg gtcgagcgcc gagcacggct cgtccatgag cagcacttgc
     1861 ggctcgacgg cgatcgcgcg ggcgatgcac agacgctgct gctggcctcc agacagcccg
     1921 gcgcccggct tgttcagccg atccttgacc tcgttccaga ggttcgcgcc ccgcaacgac
     1981 cgctcggccg cgtcggcgag ttccgacttg ttgcgcaggc cgttgagccg cagcccggcg
     2041 acgacgttgt cgaagatcgt catcgtgggg aaggggttag gccgctggaa gaccatgccg
     2101 acggtgcggc gcacggccac gggatcgacc tccgcggcat acaggtcctg gccggcgagg
     2161 gtgatgctgc cctccacccg ccctccgggg atcacttcgt gcatgcggtt aagggtgcga
     2221 agcaccgtcg acttgccgca gccggaggga ccgatgaacg ccgtgaccga gcgcggttcg
     2281 aacgtcaacg tgacgccctc caccgccttg aacgcgccgt agtagatatt gacgtcctcg
     2341 accttgagcc ggttggccat ggggagtctc gctttcaggg gtgggcgcgt cagcgccgga
     2401 cggaggagcg ccgggcggcc cagcggccga tgaggttgag cgcgaggacg atgaggatga
     2461 gggtgagcgc ggcggcccac atccgctcca tggcgctgtc caggccgatc tcggtgcggt
     2521 cttggttgat catcgtcggc agcgtcggca tgtagccgtc gaagaggttn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgaaggtggt gacccacacg gcatacacaa
     2701 agagcgcggc aacgatggac ggcaccccac tgaggatgtc gaccatgaac gacacgacac
     2761 gagcgagacg gccgcggccg tactccacga ggtagatcgc cgtgaagatg cccagcggca
     2821 ccgagatcgc tgcggtcact gcggcctgga tgagggtgcc ctggatcgcg tgaactgcgc
     2881 cgccgcccac gtcggccgcg ttgatgttgc gctgcgacag ggtccaccag gagctggcca
     2941 gcagcaactc gtagccgcgg gccaccaccg tgccgaggat ccacacgagc gggaccatcg
     3001 cgagcccgaa ggacgcccac atgaccacgc gcgcaacctg gtcggtcagc cgccgggatg
     3061 cagaccctcg cgtgagctgc ggcgggtcgt cggtcacgac atccgctcgg tatgccgcgc
     3121 gcctggcggc cacgaggcgc gccagcgggg tgcccgtcgc ctcggtggtc tctgcggcgc
     3181 tggtcgcgct cattcggtga aggccttcct acgctcgatg acgaccctcg caacggcgtt
     3241 gacaacgaag gtcaggatga acagcacgag gccggccgcg atgaaggcgc ccgtcttctg
     3301 cggcgagtcg aactcggcgg cgttgttggc gatgcgggag gcgaacgtct ccccgccgct
     3361 gagcatcgac caggtccagt cggagccggc ggccagcgtc gacacgatga acgtgacggc
     3421 gatcgtctcg ccgagggcac ggcccagggc caacatggcg gcgctgatga ctccgggctt
     3481 gccgaacggc aggaccgcgg tgcggatcat ttcccaccgg gtggccccca gcgccagggc
     3541 accctctttg tgagtggtgg gcgtttgggc gaacacctct cgggagatcg cggtgacgat
     3601 gggcaggatc atgatggcca ggaccacgcc gatgaacatg atcgtgccgc cggtgatgcc
     3661 ggtcggagcg aacagtggca gccacccgag acgggtcgag agccagtcct cgagcggccg
     3721 cacgaccggg cggaagatga tgagccccca gaggccatag acgatcgagg ggactgctgc
     3781 gagcagatcg accagggtcg cagcgggccg ccgcagccac tgcggcgcgt attcggtgat
     3841 gaacagcgcc acgcacaccc caatggggac ggcgatggcc atcgcgatga tcgacgccgt
     3901 cgtcgtcgtc cagagcaact ccatgatgcc gaaccgcggg ttgtccccgc cgaccgacca
     3961 ctcacgggag gtgaggaagt tggcctcgtt cttcgacagg gcggggatgg cctgcgagac
     4021 caggaacacg ccgacgagcg tcacgagggc caccaccgcc acgccggccc cttgcgaggc
     4081 cagcccgaac cagcggtccc caccccgacg aggggccgtg acggtggcgg tccacgagga
     4141 acccgctggt tcggtcaact ctggtggagt tgtggttgca gacacgtcaa tgtccccgag
     4201 aaatccgtag cctcagccaa tggccgcgac ggcggccgtg atcttggcgg agaccgagga
     4261 ggggagcgct gcgtagccca gggcctcgag cgacttctgc acgtcggccg agacgaagtg
     4321 cgtgaggaac gacttcacca gcttgccctt ggccgcgtcc ttgtacttcg agcagacgat
     4381 ttcgtaggtc accaggttga tcgggtaggc cccgggggtc ttggtggcgt agtccagcgt
     4441 gagcgagagg tcgttgccgg tccccttctg ggtggccgtc tcgatcatct tgccgaccga
     4501 cgcgctcgtg agctcgacgg gaccggaccc gttgtcgatc ttggccaggc cgagcttgtt
     4561 ctgggtgacg taggaccact ccatgtagga gatgccgccc tcgacgctct tggcggccgt
     4621 ggccaccccg gcgttctgcg gcttgccctc gccgaccgtt ccgttccact tcttgcccga
     4681 ctcgaccgtc caggcggtgc ccgcagcggc cttgaggtac ttcgtgaagt tgtcggtcgt
     4741 tcccgactcg tccgagcgga agaagacgct gatcttcgtc gccggcaggg tcgtgccaga
     4801 gttgagcgcg gcgatcgccg ggtcgttcca catggtgatc tggccgagga agatcttcgc
     4861 ggtgacgtcg gcgttgagga cgagcgagga caccgacggc aggttgtagg cgatgccgac
     4921 cgggccggtc accatcggca ggtgccaggc cggggaggcg caggtcgtct cggcggactt
     4981 cgcctcgtcc tccttgagcg cggagtcgga gccgccgaag tcgacctgac cggcgatgaa
     5041 ctgcttgatg ccggcaccgg aacccgtcgc cgcgtagttg acggtggtgc cgctgcactt
     5101 ggtctggaag ctcttgatcg ccgcttcgat ggcgttcttc tgggccgagg atccctcggc
     5161 cttgagggtg cccgtcgggc agtcggacgc agcggtgccg gtggccgcgg tgccagtctt
     5221 cgtggcgtcg gagccacaag cgcccagggc caaggccccg gacatcgcga cgatgatgat
     5281 ggcgctgctt cgggtggtca tcacgggggt gtccctctca cagggtcagc gagacggtct
     5341 gcctcggctg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacctcgcc
     5461 cttctcgatc ccgaggtcgg cggcctcggc gagggaggtg tcggcgccgg ggatcaactc
     5521 gtcaacgggt gccaggcggc ccacgagggc ttcgaggatc gtggggagca ccggcccgtg
     5581 gctgcacaca gccgtaggaa ctccggtggc cagcacctca tcgacgaccc ggttgacgcg
     5641 ctttgcgccc cgcttggcga agccttcctc ggagatggcg ttgcgcatgt gcaggtccag
     5701 gccgctgcgc ttggcatatc ccgcgagggt ttgcacgcat cgggtcgacg gtgaggtggt
     5761 cagcgcttcg acgccgtatg ccgccaacgt gtcggtgatg agcggcacct ggacgtgccc
     5821 cgcgggggat aacggacgct tgcggtcgtc gcccttccac gccgaccgtg gggtcgccag
     5881 ggcatgccgc acgacgagca gcggccaggt gtcgagcaac ccgcgctgat aggcccgaag
     5941 cagcgcccgc aactgttcgc ggtcgtgcga gtagtccagc cgggtgtcgg ccgacgtcgc
     6001 gtcgagccag gccacctcgt cgatctcgtg agtgaggctg ccatctccgc cggtgacctg
     6061 ggccgcccaa tagcggaccc acttggtagc ggggacgccg tcgcgatcca ggacgcgata
     6121 ggtggtcggg gggagcggca tacccagacg gacctgcagc cccgtctcct cctgcgtttc
     6181 gcggaacgca gcaaccggcc agcgctcacc cggctcgagc ttgcccttgg accacgacca
     6241 gtcgtcgtag cggggtcggt ggacgagcgc cacttccagg gcatcgccgt tgacgcgcca
     6301 cgggacggtt cctgcagcgt tgatcgtcgg cacgggggaa ccctatcgac gtcgggcttt
     6361 gcggcgcgcc ttgttgtggt ggtcccacat gagggtttgc agatcattga gcggccggcc
     6421 ctcggcatcc cggtagaccc gggtccacgt gccgtcggcg ccgagctcga agcgggaggt
     6481 ggtgtcggcc aaccccatgt tgaacatgtt ggtcagctca gcgacgtggc tcgtgtcgac
     6541 gatctgcacc aaggcctcga cgcgacggtc gaggttgcgg tgcatcatgt cggcgctgcc
     6601 gatgtagacc tgcgggctcc cgccaccggc gaaccagaag attcgcgagt gttcgaggaa
     6661 tcgcccgagc gtggaacgca cccgcaggtt gtcgctgagg ccgggcacct gcggccggat
     6721 ggcgcagatc ccacggaccc agacgtcgac ggggacgccg gcttgcgacg cgcggtagag
     6781 ggcatcgatg agttgctcgt cgacgatgga gttcatcttc agccggatgg cgccgccgcc
     6841 atcggcctcg tgccgctcaa tctcggcgtc gaccaactcg atgagcccgg aacgcaccgt
     6901 ccgcggcgcc acgagcaacc gccggaaacg gctgcgcggc gcgatgcccg agagctggtt
     6961 gaacagccgc gagaggtcct cgccgacctg cgggtcgcag gtgaggatgc cgaggtcttc
     7021 gtagaggcgg gccgtcttgg ggttgtagtt gccggtgccg acgtggcagt agcgcaccaa
     7081 cccctcagtc tcctggcgca cgacgagaca gagcttcgcg tgcgtcttga gaccgacgat
     7141 gccgtagacg acgtggacgc cggcgtgctc gagcttgcgc gcccagctga tgttgttgac
     7201 ctcgtcgaag cgggccttga tctcgacgac ggcgagcacc tgcttgccgg ccgtggccgc
     7261 gtcgatgagc gcgtcgatga tcggcgagtc accggaggtg cgatagagcg tctgcttgat
     7321 cgccagcacc cgcgggtcgg cggcggcctg gccgatgaag gactgcaccg acgtcgagaa
     7381 cgagtcgtag gggtggtgca gcaggatgtc cttcgagcgg atcgccgcga agaggtcgcc
     7441 ctcctgcgag cgctcggtcg gggccaagtc cgggtgggtc atggggacga acggcgggtc
     7501 cttgagctcg gaccggtcga ggtcaccgag gaggaacagg ccgcgcaggt ccagcggggc
     7561 ggggaggcgg taggtctcgg cctcggtgac acccaactcg cgggtgagca ggtccaacac
     7621 gcggtcggcg atggcgtcct cgacctcgag ccgcaccggc gggccgaagc ggcgtcgggt
     7681 caactccttc tccaacgcgg tgaggaggtt ctcggcgtcg tcctcttcga cctcgaggtc
     7741 ttcgttgcgg gtcacccgga aggtgtagtg ctcgcgcacc tccatgccgg tgaacaactg
     7801 gtcgaggtgg accgcgatga cgtcttccag ggggatgaac cgggcgcgct gggtgccgtg
     7861 gtcatcgccg ggctcgaaca cccgaaggaa ccggggcagc accggcggca ccttgacccg
     7921 ggcgaagtgc tccgcaccgg tcttggggtt gaccaggacg acggcgaggt tgagcgacag
     7981 accggagatg taggggaacg ggtgggccgg gtcgacggcc agcggggtga gcaccgggaa
     8041 gatctgctcg cggaacagcc cgtgcaacgg cgcgcgctcg gcctcgttga ggtcgcccca
     8101 gcgcacgatg ctgatgccct cttcgtgcag cgccggacgg atctggtccc agaagaccca
     8161 cgagtgcatc cgcatgagtt cctgcgccgc gtgctggatc cggtcgagca actcgcgcgg
     8221 ctcaagccca gcggccgagc ggacggcgag gcccgtggcg atgcggcgct tgagcccggc
     8281 gacccggacc atgaagaact cgtcgaggtt gctggagaag atcgcgagga acttcgcccg
     8341 ctcgatgagg gggacggtct cgtccatcgc caactgcagc acgcgctcgt tgaactgcag
     8401 ccacgagatc tcgcgctcga ggtagcgctc ctgcggcaga tcgagggcga ccacgggccg
     8461 cgcctcgacc tcatcggccg ccgcggagtc gggcatccga cccccgagcg gcgccgcagt
     8521 gaggggctcg gtcaacggtg ggacgggtgc ttcgctctcg actcgcgcgt cggcgctggc
     8581 gctcatgggg accatcgttc cactaacaac aggtggcgtg tgcgtgaaca tcgtctcgtt
     8641 cgcgaggctc tggaacccca gcgaatcgta ggtgcgccgg gccgcctcgt tgtctccgtc
     8701 gacgtaaagg aacgcttcgc cgatccccgt ttcccgcagg tgatgcagcc ccagcagggt
     8761 catcgagcgg cccagtccgc ggccttggta tgccgggtgc acggccacgg catacacctc
     8821 accgcggcgc gtggctgggt cgtcggtggg accgtcgtgg accttcgtcc agtggaagcc
     8881 ggcgaccgtg gggccctcgc ccgcgcggga cgggacgacc atgagtagcc cggccgggtc
     8941 gaaccacggc tgagccatcc gggcgcgcag gtcggcgacc gtcagccgac cctgctcggg
     9001 gtgggaggcg aaggcagcgg cgttgacggc gacccaggcc tgctcgtcgg tccccggccg
     9061 gaagggacgc acgacgagcc cctcgggaag ctcggcgggc ggcaacgagt cgtcgagagt
     9121 gaggccgagg atgtgcaggt cgcgcaccgg cgtcaggcct cgcgccttga cgaacgccgt
     9181 tgcggcggcg ctggccccgt gggcccagat gcgcacccct gacggcatgg ccgacaacag
     9241 ggccgtcgca atgccctcgc gtcggtgctg ggggtggacg acgatctcgg ccgcgctgtc
     9301 ggggtcgtcg gggtcggtca gctgggcgta cccgacgagt tgcgaaccgg catacaggag
     9361 cagatgtccg gcacccgagg tggaggccaa cccgagcagg aaagcctcgg agagcggagg
     9421 agcgccgtcg gcggcctcgg ccgctgccac gagaccgcga accgcctcgg cccgctcagg
     9481 gcgcagggag cggacggcca ggggtgcgga ggcgagcggt gttgcggcga gggggtgcga
     9541 cggcataccc gattccatca tgaaccggtc ggtgtcagac cccggcatcc acctcggcga
     9601 cgatggtgtc gcggggacgc tcagggacga agcggtaacc gacgttgcgg accgtcccga
     9661 tgagggtctc gtgctcgggc ccgagcttgg cgcgcaggcg tcggacgtgg acgtcgaccg
     9721 tgcgggtgcc gccgaagtag tcgtagcccc agacctcctg cagcaactgg gcgcgggaga
     9781 agacccggcc cggatgctgc gcgaggaact tgagcagctc gaactctttg taggtgaggt
     9841 cgagggcacg cccacggaac cgcgcggagt agctcacctc gtcgatcgtc agatcgccgg
     9901 cgcggatcgg cccggcctcc tgcgcctcct cggtctcgac acgggtgaac gccagccgca
     9961 gacgcgcgtc gacctcggcc ggacctgccg tgtcgagcag gacgtcgtcc aggccccagt
    10021 cggcgttgag ggcggcgagg ccgccttcgg tgaggacggc catgaggggg gtggagatcc
    10081 cggtggctcg caggacccga cacagcgact tggcgtgggc gaggtcgcgg cgggcgtcga
    10141 cgagcacagc gtcgccgggc ggggcgtgca ccagcgcggc cggctcggcc ggcaggatgc
    10201 gaacctggtg gcccaacagg gcgagcgccg gcagcacctc agcgctgggc gccacggtgt
    10261 tggtcagcag caacaacatg gccacagggc gaggataatc gaggcgtccg acatgcctcg
    10321 ggtctgtcct gtgagtgaga ccgcttgcat aggctgtgcc agtggtccgc gcgcaccgtc
    10381 gcgggtggtc gaaaggggtc ggtatgccgt ccggcacagt gctgctgcgc tactgggctg
    10441 ccgctcgggc ggctgcgggc gtcgacaccg aagccgttcc ggtgcccgtc gagggcgagt
    10501 cggtcgggcc gttgctcgac gcggccgtca tccgtcaccc cgagctggcc cgcgtcctga
    10561 aggtggcctc cgtgctcgtc gacgggcggg ccgccgaccg tgacacgcgg gtggctccgg
    10621 cgggggtcgt cgaggtcctt ccaccgtttg cgggcgggtg atgcgacgat gtcgggcgtg
    10681 agtgacccct ccgcggccac cagccaccgt cgtggccccc acgtcgggcg gttcggcgac
    10741 gtcgactcga cccggttgcc cggcctggtc accgtcgctg ccctgacaac gaccggggcg
    10801 ctggcggcaa cctccctggc agggccgggg ctgctcgccg ggctgctcgg catcctcgcc
    10861 ctcatcgtcg ccctggggtg gccgatcctc agcagcccac ccgcgctggg gcaggttcaa
    10921 gtcggcctcg ccgtcggcgg tctgctcatt gcggcgacgg tcggcctgac gaccgggtcg
    10981 caccgactcg tctggcttcc cgtcgcggtg gccatcggga tgctcgtgtc gttcttcctg
    11041 caactgctgc gatccgacga ccggcacggt ctcaccgagg cgatgtcggt ggtcgcgacc
    11101 gggctggcgt gcgtcgggtc cggcgcggcc ctcgtgcccc tcatcctcac cgagcctggc
    11161 gtgcggttcg ccctcatcgg gttggccggt ctcgctgccg gagtgcttgc cgagctcacc
    11221 ggtcggatgc cgcgggtcgg ggccaaggcg gtgttcgtcg tcatgctcgc cggcggtttg
    11281 ggcgggtggc tggctgcggt gctgctcggg tcggccgggt cggctggcct cggcgccggc
    11341 atgctcctcg cgtccttctc ctactccgcc cggcgcatct tcggcgccgt ccccggctct
    11401 cgggaggccc ccggacaggt ggccctcggg gtcgcgaccg tgctgctgcc caccgtgttg
    11461 ctcctcgccc ttggtgcgct ggccaaggtc tattccggct gacgtccgat cccgcggcgc
    11521 tgctggtcag tagaccaggg cctgggctcc ctcggcgagg gcctgctcga cgaaccccgc
    11581 agccccggcg atccgggcgc ccgggagcag gtcggcttcg gtcaaacccc gccgcgccgc
    11641 gcactgggtg cacaccgtga cgacctcggc gtcgagcagc aggtcgacca ggtcctcgaa
    11701 cggcgcagcg tgcgggaggg tgaggcgcgc ggcataccca ggggtcgcga ggcgggtcgc
    11761 ctccccggtg agccacacgc ttgtcggtat gccgctcgcc agcgccgtcg tcgcgaccgt
    11821 cagcgcctgg gcgacgcgtt cgagctcggc ggtcgggggc ggccaggtgt cgccgagggc
    11881 ggtgagtttg acgacgaggg aacgggccat ggggccagac tacgatcggg gaaccggtcc
    11941 ggaaggggtc ctcatgttcg agctcgacgt caatctgccc gagcggctgc ggcccctggc
    12001 ctggctcatc ggtcggtggg agggcgccgg agtgctgggc tatccgacca tcgagtcgat
    12061 gcactacggc caggaaatcc gctgctggca tgacgggcgg gccttcctgc aatgggaggc
    12121 gcgggcctgg atcctcgact ccgagaacgg cgagcgggtg cggccggcat cgatcgagac
    12181 cggcttctgg cggccgggcg aggacgggcg cgaggtcgag ctgctgctgg cgcacccgac
    12241 ggggatcctc gagatgtact acgggcagat ggagccggcc aagatcaccc tgcgcaccga
    12301 ctcggtgatg cgcagcccgc acgccaagga gtacaacgcg gcgacccggc tctatgggct
    12361 ggtcgagggc gacctcatgt acacgatgga catggcagcc gtcggcgagc cgatgacccc
    12421 gcacctctcg gcccagctca agcgcgtcgg ctgaccgacg ccttagcgga gtgaaaccga
    12481 catcccagtg tcggtatccc tccggcaagg gctgcccgcg ccggacgaac cccggagagc
    12541 ccgttgcttg gggttggccg atcgctatcg gtgtcgcctt cctcatcggt ggcctcgtca
    12601 ggcggcgctc ggcctgaccg gcccacaaaa gatcgtcgac ctttcgttcg ctcgacgaaa
    12661 tatcgtcgag ggtgcaaaag gtcgacgatg ttttgccggg gtagccgcgg gagtgagtgg
    12721 agggggaggg cagctggtgg cccgagcgac gggcgagggg atcgcagggg atcgggcggt
    12781 gcgcgggcgg catacagtgg gctggtgagt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nggggatcgc aggggatcgg gcggtgcgcg ggcggcatac agtgggctgg
    12961 tgagtgacct tgcagcggtg ttgcgagcca acgggatgcg gctgacgccc cagcggcgcc
    13021 gcgtcgttga cgcgctcgcc cggctcgggc acgcgacccc cgacgcggtt gccgccgaag
    13081 tgggtgccga cgggggctcc cccctcgctg cctcgaccat ctaccgcgcg ctcgaggcgc
    13141 tcgaggagct tggcctcgtc gcccacaccc acctggacca tcgcgccccc acgttccacc
    13201 tggccgggca cgccgatcac atccacctcg tctgcctcgg ctgtcacacg atcgacgagg
    13261 tgccggtcga ggtcgcggcg gagttcgtcg cggggatggc tgcggcgcgg gggttccgcg
    13321 ccgacatcac gcacatggcc gttcacggct ggtgctcctc gtgcggaggt ccgcaatgac
    13381 cgggtatgcc gtcccctcac cccttctcga cgcccccggt gcggtggcgg ccgagggagt
    13441 cgatgccggg gtcgccgggc actacggcga cccgatgcgc gagcagcggt tgctcaccga
    13501 gggcctagcc gcggtcgacc tgtccaaccg gggcgtcgtc acggtgaccg ggcccgaccg
    13561 actttcgtgg ttgaactcga tgacgaccca ggcactcacc ggtctcacgc ccggggtcgg
    13621 caccgagtcg ctcgtcctgg acccccacgg gcacgtcgag catgcgctgc acctcgtcga
    13681 tgacggcacg accacgtgga tcaccgtcga gcccggcacc gcgcccgcgc tggtggcctg
    13741 gttggagcgg atgcgcttca tgctccgcgt cgaggtcgcc gacgtcaccg atcggtatgc
    13801 cgtcgtcggc gaacctttcg gggctgtggc tgcgcccggt gagccggtcg tgtgggtcga
    13861 cccatggccg ggggtcatcg atctgacgcc gtccgggcgg gcggggcgga tcgcggcata
    13921 cggaccagtc gacggtcacc ccgcgcaggg gcgacgttgg cgcgaggcga tcgtgcctcg
    13981 cgaggagttg gcgacctaca tcgacgaccg gccgctggcc ggagtgctgg ccgccgaggc
    14041 gctgcgggtt gcggcgtggc ggccgaggct ggggttcgag accgatcacc ggacgctcgt
    14101 gcacgaggtc gactggctgc gctcggcggt gcacctgaac aaggggtgtt accgcgggca
    14161 ggaggcggtc gcgcgcgtgc acaacctcgg caagccaccg cgccgactgg tgttcctgca
    14221 cctggacgga tcggggcatg tgctgccgcc gcgcggcgcc gaggtgacgg tcgccggtgg
    14281 ggggagcggc cccctggccg gccgagcggt ggggttcgtg acctcggtgg cgcaccattt
    14341 cgagaccggc ccgatcgcgt tggcactggt caagcgctcg ctcccggccg atgccccgct
    14401 catcgtggcc gacggcattg gggggccggg gatcgccgcc tcgcaagagg tcatcgtcgc
    14461 cccataggca cgccgcggcg cccaagtatc cggcaggagg cgtgacaagg tgaaaaatct
    14521 tcggcgaaat ggaagaatga cagcatgatg tccgcttcca gcgctgcagg cactctcatc
    14581 accgttgctc ccaccggggc cgagacggcc aaggccgacg tgccccaact gcccaccacg
    14641 ctcgacgagc tcgtcgagac ggcccggcgc tgcgaggccg cgggcgctgg tctgatccac
    14701 atccacgtgc gcgaccgcga gcaccgcccg actctcgacg cggcatacct gcgggacgcg
    14761 gtcgtggccg tgcgggaggc gaccgacctc gtcgtgcaac tctcgaccgg cggctccgtc
    14821 cacgacccac tcgagaagcg gctcaccgtg ctcgacgccg accccgattc gtgctcgttg
    14881 acctgcggga cgaccaactt cggcgacgac gtcttcctca acccgtgggg cttcatggtc
    14941 gacctctatc ggctggccca ggagcgtgag gtggtgccgg agttcgagct cttcgagctc
    15001 gggcatgtcc acgcgctgcg ccggctcatc gacacgtgcg ggctgccgtt cggcggcaag
    15061 gtgcacgtcg acttcgtcac gggggtgccc ggcgcaatgc ctggcaccgt cgaggcgctg
    15121 ctggccggtg tcgcgatgct gcccgccgag gtgaccagtt ggtcggcgac cggcatcggc
    15181 cgggcccacc tgccgatcct cggcgccgcg ctcgcggccg gggggcatct gcgggtcggc
    15241 atggaggaca accttttcgt cgcgcgcggt caggtcgccc agcacaacgt ggagttcgtc
    15301 gagcgggcgg ccactttggc tgggctgatg cagcggccgg ccctctctac cacccaggcg
    15361 cgcgagctcc tcgggatcaa ggaccgccga gcccgctgag tcacgggccc gtggcgcccc
    15421 tggccgcggc caacagggcc gggagatcga cgcacgcctc caacgcgtc
//
LOCUS       DAORPW010000048        15416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_756_length_15416_cov_807.557711, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000048 DAORPW010000000
VERSION     DAORPW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15416
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_756_length_15416_cov_807.557711"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLB86_05910"
     CDS             complement(<1..925)
                     /locus_tag="PLB86_05910"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HRC64704.1"
                     /translation="MISSLIGRAATVGAVAAIALAGFGAARLDKSIDLNVDGQLTSMH
                     VFGSTVGDALAKSGVTVGEHDLVSPAADQAIEDGSQVVVRYGRKVTVTVDGATTDYWT
                     TATTVDQAMNELGIRALSGATLSVSRSAGLGREGISFTVTNPRQVTITVDGATRAEAT
                     SAATVAELLNALGVTLGANDRVVPAADTAVTEGLAVAVQRVELRTTTATEKVAFKTVK
                     KDDATITKGQTKVQTPGVNGSQVVTFTETWVDGKLEGKSATGTDVTKAATDKVVLVGT
                     KAAPVAASAPVSAGNTSGAGINLANAAMWDRI"
     gene            complement(1220..2086)
                     /locus_tag="PLB86_05915"
     CDS             complement(1220..2086)
                     /locus_tag="PLB86_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRC64705.1"
                     /translation="MTGGGTSGAGKAARGIPPAPDPLPIDVVDNHLHLDIARHGEERG
                     DVGETIAAAAAVGVPRMVQIGCDLPGARLTIGMIEEYAALLGGVALHPNEIARFESEA
                     ALADGLAEIERLAAHPRVRVVGETGLDYFRTGPEGQPAQQDAFRWHIDLAKRLGKTLQ
                     IHDRDAHDDVLRILDEEGAPERTVLHCFSGDVGMAQRAVERGLYLSFAGTVTFSSAKG
                     LRDALSVVPLDRVLVETDAPYLTPHPWRGAANAPYLIPVTVRAMAHVLGVAVPTLCQA
                     IAANSERLYGPW"
     gene            complement(2091..3893)
                     /gene="metG"
                     /locus_tag="PLB86_05920"
     CDS             complement(2091..3893)
                     /gene="metG"
                     /locus_tag="PLB86_05920"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRC64706.1"
                     /translation="MSKVLSAVAWPYANGPRHIGHVAGFGVPSDVFSRYMRMAGHDVL
                     MVSGTDEHGTPILVQADKEGVTPRALADRFNRVIAEDLVGLGLAYDLFTRTTTPNHYS
                     VSQELFRGNHANGYMIEQTQLVAISPSTGRTLPDRYIEGTCPICGYGEARGDQCDNCG
                     NQMDPAELIDPRSKINGETPRFEETAHFFLDLPALAGALAEWLDGREATGLWRPNVIK
                     FSKNILDEVRPRPMTRDIDWGIPVPLPGWEDNPNKKLYVWFDAVIGYLSASIEWARRT
                     GDPEAWRRWWNDADAVSYYFQGKDNITFHSQIWPAELLGYAGRGDRGGEPGAAYGVLN
                     LPTEVVASEFMTMEGRQFSTSRGHVIYVRDVLERYGPDPLRYFICAAGPENQDSDFTW
                     AEFVQRNNSELVAGWGNLVNRTASMIHKRFGEIPAAGPLEPIDEELLAAIREGFDTVG
                     GHIERQRFKAGISEVMRLVGLANGYVSATEPFKLKADDQRERLGTVLHVLAQAVSDLN
                     TMTAPFLPHGANRIHQIMGGTGEFVPMPELREVDDLDGGPGYPVIMGDYGNTPRWESR
                     PVVPGTPIGPPTPVFTKFDPSVVDDEIARMRGDG"
     gene            4051..4617
                     /locus_tag="PLB86_05925"
     CDS             4051..4617
                     /locus_tag="PLB86_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64707.1"
                     /translation="MGSPVLTLSSAAQAGLRKDQIYALTESGELERIGRGVFVDPTRI
                     DPAWTSLAAATALKPAATLCLTSALVHHGLSDAIAFTTDIALPRGSRHPAGFHHVSWH
                     SFDPDTFHAGRTVTDQSGLMLAVYSAERTIIDCFRLAHHQGADQAYEALRRWVRQPGN
                     QPSELLALAAASFPRNLPRVRAALEVLL"
     gene            4716..5507
                     /locus_tag="PLB86_05930"
     CDS             4716..5507
                     /locus_tag="PLB86_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRC64708.1"
                     /translation="MYTLEGFLARLALSTQAGDFVLKGGVLMAAFADRRPTRDIDLLA
                     SGFANEIAECEGRVRDIANIPFDDGLTFDRSSVKGEFIRDEADYTGVRVHISATLASA
                     QLGIHVDISFGDPIWPGPTETDLPRLLGGTVQLLGYPDHMVLAEKLVTAIQRGTANTR
                     WRDFVDVYAIAEGRPIAQRDLRKSLATVALHRGANLRPLAQALAGMAEAAQPQWAAWR
                     RRQRLDGTTPEKFQDLLDAVIRFADPALGDEPTSRAWNPQRREWL"
     gene            complement(5621..>6787)
                     /locus_tag="PLB86_05935"
     CDS             complement(5621..>6787)
                     /locus_tag="PLB86_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRC64709.1"
                     /translation="AAPVAAQLRGELLHRVATAVATADGPAEGLADVPANGPAGGPVD
                     EPEEVLVRLDPAWVRYAPLTTSGLVSAAAIWGVSAQYIGQVDELKDRVEGAVDRVAAL
                     GVGVAALAGFLSALVAVSVLAVSGYVLTYWGFRLTRHTGGTLHISRGLITARATSLEE
                     RRIRGVEIGQPLGLRLAGAARLSAATSGLGQEGARSGSDWLTPPAPAEVVDRTAVAVV
                     GDEVAVRGPLLPHGPAARRRRFTRAILPAAVLCAATLLLTRAIDDLPTALVVAGCLPL
                     LASPWLAADRYAALGHALTPRHLIVRSGTFSRRRVVLARDGVIGVTIRESFFQRRAGL
                     ATVTATTAVGRQHYDAIDVPVELSTGLAADLLPGPFAELLDAEHVPQPSSSDRA"
     assembly_gap    6788..6887
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6888..7359)
                     /locus_tag="PLB86_05940"
     CDS             complement(<6888..7359)
                     /locus_tag="PLB86_05940"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRC64710.1"
                     /translation="MSDGIPPILAGDPQWRRLDARMLLVHPVQEAVRFLPALLVVFLS
                     GRSSDRSPWWDLGILAIIVVLGIMRWVTTRYRIWGGQIELRQGLVNRRLLTTPADRVR
                     TVDVTAPIWHRALGLARVEIGTAGGGLGAHRIVLDALAAPVAAQLRGELLHRVAT"
     gene            complement(7356..7859)
                     /locus_tag="PLB86_05945"
     CDS             complement(7356..7859)
                     /locus_tag="PLB86_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRC64711.1"
                     /translation="MTAPEIPQLREPANLVCPRARWLWLAGGVGEAVLLAALQVGWWF
                     IDQRGSRTPHIIVAVVLGVLALAYLVVMPIWRYRVHRWEVTSSAVYTQTGWISQERRI
                     APLSRVQTVDLTRGPIAQLLRLATVRVTTASAAGHLVVHGLDQDEAAALVERLTAAAA
                     AERGDAT"
     gene            7938..8411
                     /locus_tag="PLB86_05950"
     CDS             7938..8411
                     /locus_tag="PLB86_05950"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC64712.1"
                     /translation="MADVRETLHMDAPAELVYDLVADLPRMGEWSTECERVVWRGTAT
                     KAEHGAGFIGHNRAGTVRWITFGKVTAAERGRYLAFDIFVGPLQVSRWEYVFAPDEDG
                     QGCTVAEQWTDRRSAVQRTLGNRVVGERVEKNREGIRQTLRTLKRVAEAQFHPAS"
     gene            complement(8371..>9216)
                     /gene="rsmI"
                     /locus_tag="PLB86_05955"
     CDS             complement(8371..>9216)
                     /gene="rsmI"
                     /locus_tag="PLB86_05955"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRC64713.1"
                     /translation="TPIGDPRDAAPRLAQELAGADVVAAEDTRRLRRLTTDLGVTISG
                     RVLSYHEHNEAARTPELVEQLLSGARVLVVTDAGMPSVSDPGYRLVAAAVEAGVRVTC
                     VPGPSAVLMALAVSGLPVDRFCFEGFLPRKPGERARALTALADEPRTMVFFEAPHRTA
                     ATLAAMAAVWGVDRPGAVCRELTKTYEEVRRGGLGELAEWAEAGVLGEVTLVVAGRPA
                     RAPVEAGGAEFAAVVHGIQERVAAGERLKDVCADVAAATGLAKRTLYDAAVSSRGGTA
                     PRPPA"
     assembly_gap    9217..9316
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9317..10051
                     /locus_tag="PLB86_05960"
     CDS             9317..10051
                     /locus_tag="PLB86_05960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRC64714.1"
                     /translation="MLIVLGLSLGASAIWSVLRIVERLTRAVPLGQQTSSLNTSVTPD
                     RPWLDLAYQLTGIGLALVPVALCLYLLARVEPPEPGTAAYRVLGLDLRRPRLDLGGGV
                     VLAAAIGIPGLGLYLVATRLGFNTQVAPANLAGAWWTVPVLVLSALQNAVLEEVIVVG
                     YLLTRLEQMGVGARWAVGISAVVRGSYHLYQGFGGFVGNIVMGVVFALVWRRWRRVGP
                     LVVAHALIDIVAFVGYALLKDYLPWG"
     gene            complement(10074..11003)
                     /locus_tag="PLB86_05965"
     CDS             complement(10074..11003)
                     /locus_tag="PLB86_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRC64715.1"
                     /translation="MPPAPARENLTRFAWLSIAAAVATIALKFGAYLLTGSVGLLSDA
                     AESVVNLVAAFVALIALHVAAQPADVDHQFGHSKAEYFSAAVEGVMIFVAAIFILWSS
                     VQRFFAPEPLENVGMGLGISVAASALNGVVAFVLMRAGRKHNSITLTADGKHLLTDVW
                     TSVGVVVGVLLVVLTGWLRLDPIIAFLVGCNIIWTGWHLVRESVDGLMDHAPSPECQA
                     DIQAILDEFAHREGSPVLFHGVRARVAGHVQHVAFHVLVPGTWTVTQGHDLLEEIEGR
                     FHEKFGHIVVDTHLEPIEDPRSYDAPQWESASP"
     gene            11049..12620
                     /locus_tag="PLB86_05970"
     CDS             11049..12620
                     /locus_tag="PLB86_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRC64716.1"
                     /translation="MTSEEQLRRRLLGDPPPRDLLGWLGPLVAMILGGIIRFWDLARP
                     HQLVFDETYYVKQAWSMLEWGVEMRNSSAFGDKPDEAFTHGTPNVFNSTDGDLVVHGP
                     VGKWVIASGEAIFGGDSSFGWRFAVCVLGTISIFMLGRIARRLLGSSVLGTIAAVLLA
                     FEGHHFVHSRTGLLDLILMFFALGAFGALLLDRDRSRAVLARRMAAASPDRLRDHRWL
                     GPSMGWRPWRWVAGLSLGLAAGTKWSGLYFLVVFGLMTVFWDLGARRAVGVRAYGLAT
                     VVRDGIPAFFTVVPTVAVTYLVSWWGWFVSDHGYNKAWAATHPASSTWAWVPDDLRSW
                     WNYQAQILEFHRGLSSPHSYQSSPWSWLVMGRPTSMFAEYPKLGEDGCTVDMCAKVIT
                     PIGTPTIWWLGVAAVVILVFQWALRRDWRAGAILAGYAGGYLPWFLVGDRTIFQFYAV
                     AFVPYVVLAVTYVLGMIAGRRDAPWRRRQIGAGVVGGFLVLTVLLFAWFWPLYTAQVI
                     PYSQWWLHMWFPSWV"
     gene            12714..13847
                     /locus_tag="PLB86_05975"
     CDS             12714..13847
                     /locus_tag="PLB86_05975"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HRC64717.1"
                     /translation="MTGTALRGVWVAIIAVLALAGGCGLDAPTTVGPSTSVGVDPAPA
                     PTAPAENLRPAGPGLALSILPVGSSEQAITVDGRIRMYRAYRPARLARTAPLVVMFHG
                     GLGSARQAESAYGWDTLADREGFVVLYPDGIGAAWNVGGGCCGLAPAQGVNDVAFVRA
                     AIDNLRTRVSLDPGRTYAAGMSAGGMMAFRMACDTTLFAAIGPVAATQLGDCPNPAPT
                     SVMQVHGTADPTVPFEGSGTSASGSGSAPGSGLEPGLEPEPGIGPGIAQIVGPAIPTL
                     HATWRGIGRCGGDVIASIHGVTTQTAECPESRTVKLVIIEGGGADWPGVGRSGPLDAS
                     PSATAAPTATVAVPTVGPTVSPDAVAAYNTTARLWEFFKAHER"
     gene            complement(13930..15204)
                     /locus_tag="PLB86_05980"
     CDS             complement(13930..15204)
                     /locus_tag="PLB86_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HRC64718.1"
                     /translation="MSQPATVVVQAADVEDVHARTAAVCARLARAHAELVEITREVLE
                     TELWAEGGIRSPQHWLIVRAGLSPSRAREIALLAARSDELPETLSAMSDGRLGVDQAA
                     VVARYVPAAYSAQASELAELATVTQLRRSLARYAFADEAGDSPDQASTEDTKNTEEAE
                     EAPHPGPETERPRLSMSTVDGRFELSYSAPASVGALVETAIREARDSLFHAGLVDVSL
                     ADGLAEVANRSLSAVEGTSRSAKYRIYVHLDTDGGWIGKGGRLPSHIVDAMTCDGVLA
                     PVWETDGVPVSVGRSQRVVPDRTRRLIESRDQGCRYPGCVGAGHVEAHHIVHWRNGGR
                     TDLDQLVSLCPYHHDRHHEGEFTISGTPRTPTGLTFWARGGWPIRPLAPDRRVAPDRQ
                     ERPPATRWVGPTGERLQTKFLLLGRNPSPRAP"
BASE COUNT         2324 a         5272 c         5317 g         2303 t
ORIGIN      
        1 cgatccggtc ccacatggcg gcgttggcga ggttgatgcc cgcgcccgag gtgtttccgg
       61 cgctcaccgg ggcggaggcg gcaaccgggg ccgccttggt gccgacgagg acgaccttgt
      121 cggtcgctgc cttggtgacg tcggtgcccg tcgcggactt gccctcaagc ttcccgtcaa
      181 cccaggtctc cgtgaaagtg acgacctgcg agccgttgac gcccggggtc tggaccttgg
      241 tctggccctt cgtgatcgtc gcgtcgtcct tcttgacggt cttgaaggcc accttctcgg
      301 tggcagtggt ggtgcgcagc tcgacccgct ggacggcgac agccagtcct tcggtgacgg
      361 cggtgtcggc ggcgggaacg acgcggtcgt tggcgccgag cgtcacgccg agcgcattga
      421 gcagctcggc aaccgtcgcg gccgaggtcg cctcagcgcg agtggcgcca tcgacggtga
      481 tcgtcacctg gcgcgggttg gtcacggtga aggaaatccc ctcacggccg aggccggcgg
      541 aacgcgagac cgacagcgtc gcgccggaca gcgcgcggat gccaagctcg ttcatcgcct
      601 ggtccaccgt ggtggccgtc gtccagtagt cggtcgttgc gccgtcgacg gtcacggtga
      661 ccttgcggcc ataccgcacg acgacctgac tgccgtcctc gatggcctgg tcagccgcag
      721 gggagacgag gtcgtgttcg ccgaccgtca caccgctctt ggcgagggcg tcgccgacgg
      781 tcgagccgaa gacgtgcatc gaggtgagct ggccgtcgac gttgaggtcg atggacttgt
      841 cgaggcgagc ggcgccgaaa ccggcgaggg cgatggctgc gacggcaccg acagtggcgg
      901 cacggccgat aagactgctg atcacaatgc tccgaacgtt ggacgacccg ggcgggcacg
      961 agacgccgtg agcggtgaag gcacgtccca ggtgggccgt gtcctccgcc tccgagccct
     1021 gtcaccaggt ccagacacgg cgccgggaaa ggggtggtgc ctcccggcga tctgactcaa
     1081 cggtgacgga cgagcgcccg aaggtcaact tttggtaacg gaactccgct tggcaagtga
     1141 cgcaggtcac agaatcggcg cctcaataac cccatatgtc acacccgttg ggagtagaga
     1201 gattcttact cgaagccgct caccagggtc cgtagaggcg ctcggagttg gcggcgatgg
     1261 cctggcagag ggtcggcacg gcaacgccga ggacgtgggc catggcgcgg accgtcacag
     1321 ggatgaggta gggcgcgttg gccgcccccc gccacgggtg cggggtgaga tagggcgcgt
     1381 cggtctcgac aaggacccgg tccagcggca cgaccgaaag cgcatcccgc aggcccttgg
     1441 cgctggagaa ggtcaccgtg ccggcgaagg acagatagag gccgcgttcg acggcccgct
     1501 gggccatccc cacatcgccc gagaagcagt gcagcaccgt gcgctccggc gcaccctcct
     1561 cgtcgaggat ccgcaggacg tcatcatgcg catcgcggtc gtggatctgc agggtcttgc
     1621 ccaagcgctt ggccaggtcg atgtgccaac ggaacgcgtc ctgttgcgcg ggctgcccct
     1681 cggggccggt ccggaagtag tcgagcccgg tctcccccac gacccgcacc cgagggtgtg
     1741 ctgcaagtcg ctcgatctcg gccagcccgt cagcgagcgc cgcctccgac tcgaaccggg
     1801 caatctcgtt ggggtgcaag gcaactccgc cgaggagggc ggcatactcc tcgatcatcc
     1861 cgatcgtgag ccgcgcgccg ggcaggtcgc acccgatctg gaccatccgc gggaccccga
     1921 ccgcggcagc cgcggcgatg gtctcgccga cgtcgccgcg ctcctcaccg tggcgcgcga
     1981 tgtccaggtg gaggtggttg tcgacgacgt cgatgggcag tgggtcagga gcgggaggta
     2041 tgccgcgcgc ggccttcccg gcgcccgacg tccctccccc agtcaccggc tcagccgtcc
     2101 ccgcgcatcc gggcgatctc gtcgtcgacg acgctcgggt cgaacttcgt gaagaccggg
     2161 gtcggcggcc cgatgggcgt gcccgggacc accgggcgcg actcccaccg cggggtgttg
     2221 ccgtagtcac ccatgatgac cgggtagccc ggcccaccgt cgaggtcgtc gacctcgcgc
     2281 aactcgggca tcgggacgaa ttcgcctgtg ccgcccatga tctggtggat ccggttggca
     2341 ccgtgcggga ggaacggcgc ggtcatggtg ttgaggtcgc tcaccgcctg agccagcaca
     2401 tgcagcaccg tcccgagccg ctcgcgctgg tcgtcggcct tgagcttgaa cggctcggtc
     2461 gccgagacgt agccgttggc cagcccgacc agacgcatga cctcgctgat accggccttg
     2521 aaccgctgcc gctcgatgtg cccaccgacg gtgtcgaaac cctcgcggat cgctgcgagc
     2581 aactcctcgt cgatcggctc cagcggcccg gccgcgggga tctcgccgaa ccgcttgtgg
     2641 atcatcgagg cagtccgatt gacgaggttg ccccacccgg cgacgagttc agagttgttg
     2701 cgctggacga actcggccca ggtgaagtcg ctgtcctggt tctccgggcc ggccgcacag
     2761 atgaagtagc gcagcgggtc ggggccgtag cgctcgagga cgtcgcgcac atagatgacg
     2821 tggccgcggc tggtcgagaa ctgccgcccc tccatcgtca tgaactcgct cgccaccacc
     2881 tcggtcggca ggttgagcac cccgtatgcc gccccgggct cgccaccgcg gtctccgcga
     2941 ccggcatacc cgagcaactc ggccggccag atctgggagt ggaaggtgat gttgtccttg
     3001 ccctggaagt aataggacac cgcgtcggcg tcgttccacc agcggcgcca ggcctcgggg
     3061 tcaccggtgc ggcgggccca ctcgatcgac gccgagaggt agccgatgac cgcgtcgaac
     3121 cagacgtaga gcttcttgtt ggggttgtcc tcccacccgg gcagcggcac cgggatgccc
     3181 cagtcgatgt cgcgggtcat cgggcgcgga cgcacctcgt cgaggatgtt cttggagaac
     3241 ttgatgacgt tgggtcgcca cagcccggtc gcctcgcgcc cgtcgagcca ctcggcgagc
     3301 gcgccggcca gggccggcag gtcgaggaag aagtgggcag tctcctcgaa gcgcggcgtc
     3361 tcaccgttga tcttgctgcg cgggtcgatg agctcggccg ggtccatctg gttgccgcag
     3421 ttgtcgcact ggtcgccgcg ggcctcgccg taaccacaga tcggacaggt gccctcgata
     3481 tagcggtcgg gcagggtccg ccccgtcgac gggctgatcg ccacgagttg ggtctgctcg
     3541 atcatgtagc cgttggcgtg gttgccccgg aacaactcct gactcacgga gtagtggttg
     3601 ggcgtcgtcg tccgggtgaa caggtcgtag gccaggccca acccgacgag gtcctcggcg
     3661 atgacccggt tgaagcggtc ggccaacgcg cgcggggtga cgccctcctt gtcggcctgg
     3721 acgaggatcg gcgtcccgtg ctcgtcggtg cccgacacca tgagcacgtc atggccggcc
     3781 atccgcatgt agcggctgaa cacgtcggag ggcacaccaa aaccggcgac atggccgata
     3841 tggcgcgggc cgttggcata cggccaggcg acggcggaca ggaccttgct catgggctcg
     3901 atcctagagg tctgcagcaa ccacgtttcc gggctcacgg ccaacagcca gtggcgcgga
     3961 gtcgggcgct ccgtcgcgat gagctccaac ctgcacaccg tctctaactg tttacagata
     4021 tgattcacct gtagacagtt aggagcgacc atgggcagtc cagtgctcac cctctcctcc
     4081 gcagcgcagg cgggacttcg gaaggaccag atctacgcgc tcacggagtc cggcgaactc
     4141 gagaggatcg ggcgcggggt cttcgtcgac cccacccgga ttgatcctgc ctggacatcg
     4201 ctggccgcgg cgacggcact caagccggcg gccaccctgt gcctgacaag cgccctagtg
     4261 caccacggcc tgagcgatgc catcgcattc actaccgaca tcgcgctccc gcgcggcagc
     4321 cgccatcccg ccgggtttca ccacgtgagc tggcattcgt tcgaccctga caccttccac
     4381 gccggcagga ccgtgaccga ccaaagcggt ctcatgcttg ccgtctactc cgctgaacgc
     4441 acgatcatcg actgtttccg cttggcccat caccaggggg cagaccaggc ctacgaggcg
     4501 ctgcgccgct gggtccgtca gccggggaac caaccatcgg agctgttggc actggccgcc
     4561 gcctcattcc cgaggaacct gccccgcgtc cgcgcggccc tggaagtcct cctgtgacgc
     4621 ccaggccaac ccgagacaca ccggcaggac gggcgtacaa cgatcttcgc aatcttgcgc
     4681 gaaggcaggg ccgggatgtc gcggagtacc tcacgatgta caccctcgag gggtttctcg
     4741 cccggttggc cctctcgacg caagcgggtg acttcgttct caagggaggc gtgctgatgg
     4801 cagctttcgc cgacaggcgg cccacccgcg acatcgatct gttggcatcc ggcttcgcca
     4861 acgagatcgc cgagtgcgag ggccgcgtcc gcgacatcgc caacatcccc ttcgacgatg
     4921 gcctgacatt cgaccggtcc tcagtcaagg gcgagttcat ccgagacgaa gccgactaca
     4981 ccggtgtccg cgtccacatc agcgcaaccc tggccagcgc gcaactggga atccacgtcg
     5041 acatcagctt cggcgatccg atctggcccg ggccgacaga aaccgatctt ccccgcctcc
     5101 tcggtggaac ggtccagctc ctggggtacc ccgaccacat ggtcctcgcc gagaagctcg
     5161 tcaccgccat ccagcgcgga accgccaaca cccgctggcg cgacttcgtc gacgtgtacg
     5221 cgatcgctga gggccggccc atcgcgcaac gagatctgcg caaatcactg gcgaccgtcg
     5281 ccctgcaccg aggggcaaat ctccgccccc ttgcccaagc cctcgccggc atggccgaag
     5341 ccgcccagcc ccagtgggcg gcctggcggc gcaggcaacg cctcgacggc accacaccgg
     5401 agaagttcca ggacctactc gacgcagtga tccggttcgc cgacccggca cttggcgacg
     5461 aacccacgag ccgggcatgg aatccgcaga gaagggaatg gttgtgaccg tcctggggat
     5521 tcagcccccg ggcgttgaag cgcgagaagc gaagaagccc ccgcgcgact gatacccctg
     5581 ctcagatttg gctgacggcc gttcggccct cggtaggaag ctaggcgcgg tcactcgagc
     5641 ttggctgagg gacgtgttcc gcgtccagca actcggcgaa cggaccgggc agcaggtcgg
     5701 cggccaagcc ggtcgacaac tccaccggca cgtcgatcgc gtcgtagtgc tgccgcccca
     5761 cagccgtcgt cgccgtcacg gtggccagcc cagcgcggcg ttggaagaac gactcccgga
     5821 tcgtcacccc gatgacgccg tcccgggcga ggaccacgcg gcgccggctg aacgtccccg
     5881 atcggacgat cagatgccgc ggggtcagcg cgtggccgag cgcggcatac cggtcggcgg
     5941 cgagccaggg cgaggcgagc agcgggagac agcccgcgac gacgagcgcc gtcggcaggt
     6001 cgtcgatggc acgggtcagc aacagggttg cggcgcagag gacggccgca gggaggatgg
     6061 cgcgggtgaa ccgccgacgc cgagccgcgg ggccatgtgg cagcaaggga ccgcggaccg
     6121 ctacctcgtc tccgaccacc gcgacggcgg tgcggtcgac gacctcggcg ggcgccggag
     6181 gggtgagcca atcgctgccg ctgcgggcac cctcctggcc cagcccgctc gtcgctgcgc
     6241 tgagccgggc ggcacccgcg aggcgcagac ccagcggctg gccgatctcg acgccgcgga
     6301 tccgtcgctc ctccaggctc gtcgcccgcg cggtgatgag gccgcggctg atgtgcaagg
     6361 tgccgccggt atgccgcgtg agccggaacc cccagtaggt gaggacatac cccgacacgg
     6421 ccagcaccga cacggcgacc agcgccgaaa ggaaaccggc gagcgccgcc accccgacgc
     6481 cgagcgcggc gacccggtcg acggcaccct cgacccgatc cttgagttca tcgacctggc
     6541 cgatgtattg cgcggagacg ccccagatgg cggctgccga caccagccca ctggtcgtca
     6601 agggggcgta gcggacccac gccgggtcca gcctgacgag gacctcttcg ggctcgtcga
     6661 ccggcccgcc agccggcccg ttggctggta cgtcggccag cccctcagcc ggcccgtcag
     6721 ccgtcgccac ggcggtcgcc acccggtgca gcaactcccc gcgcagctgc gccgccaccg
     6781 gcgcggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg tcgccacccg
     6901 gtgcagcaac tccccgcgca gctgcgccgc caccggcgcg gccagggcgt cgaggacgat
     6961 ccggtgtgcg cccaacccac cgcccgcggt tccgatctcc acccgggcca gtccaagagc
     7021 ccggtgccag atcggcgcag tgacgtcgac cgtgcggacc cgatcagccg gcgtcgtcag
     7081 cagtcgccgg ttgaccagcc cctggcgcaa ctcgatctgg ccaccccaga tccgatatcg
     7141 ggtcgtgacc cagcgcatga tgcccagcac cacgatgatg gcgaggatcc cgaggtccca
     7201 ccacgggctg cgatcgctgg agcgcccgga caggaagacc accagcaggg ccgggaggaa
     7261 ccgcaccgcc tcctgcaccg ggtggacaag gagcatccgc gcatcgaggc ggcgccactg
     7321 cgggtcgcca gcaaggatcg gaggtatgcc gtcgctcacg tcgcgtcccc gcgctcggca
     7381 gccgccgcgg cggtcaaccg ctcaaccagg gccgctgcct cgtcctgatc caaaccgtgg
     7441 acgaccaggt gaccggcggc ggaggcggtg gtgacccgca cggtcgccag ccgcaacagt
     7501 tgggcgatcg gccctcgggt gaggtcgacg gtctggacgc gggagagcgg ggcgatccgg
     7561 cgctcctggc tgatccaacc ggtctgggta tagacggcac tcgacgtgac ctcccaccga
     7621 tggacccgat agcgccagat cggcatgacg acgaggtagg cgagggcgag caccccgagc
     7681 accactgcga cgatgatgtg cggggttcgg gagccgcgtt ggtcgatgaa ccaccacccc
     7741 acctggaggg cggccagcag cacggcctcc cccacaccgc ccgccagcca caaccaccgc
     7801 gcgcgagggc acacgaggtt ggcgggctcg cgcaactgcg ggatctccgg ggcggtcatg
     7861 tgtccagcag atcacacgtg ctcgacacac ggcaccccga agcggcggca cacaggcggc
     7921 tgggacagga tggtcccatg gccgacgtgc gcgagaccct gcacatggac gcgcccgccg
     7981 agctggtcta cgacctcgtc gccgacctgc cccggatggg tgagtggtcc accgagtgtg
     8041 aacgggtcgt ctggcgtggc acagcgacga aggccgagca cggggccggc ttcatcgggc
     8101 acaaccgggc cggcacggtc cggtggatca ccttcggcaa ggtgaccgcc gccgagcggg
     8161 gccgctacct cgccttcgac atcttcgtcg gaccgctcca ggtctcgcgg tgggaatacg
     8221 tcttcgcccc cgacgaggac ggtcagggct gcaccgtggc cgagcagtgg accgaccggc
     8281 gctcggccgt ccagcggacg ctgggcaacc gggtcgtcgg cgagcgcgtc gagaagaacc
     8341 gcgagggcat ccgccagacc ctgcgaaccc tcaagcgggt ggccgaggcg cagttccacc
     8401 ccgcgagctg actgccgcgt cgtagagcgt ccgcttcgcc agaccggtcg ctgccgcgac
     8461 gtcggcgcag acgtccttga gccgctcccc cgcggcaacc cgctcctgta tgccgtgcac
     8521 caccgccgcg aactccgcac cgcccgcctc gaccggggct cgggccggtc ggccagcgac
     8581 gacaagggtc acctcgccga ggaccccggc ctcggcccac tcggcgagct ctcccagccc
     8641 gccgcggcgg acctcttcgt aggtcttggt gagctcgcgg catacggcac cgggacggtc
     8701 gaccccccag accgcagcca tcgcagcgag cgtcgcggcg gtgcggtgcg gtgcctcgaa
     8761 gaagaccatg gtccgcggct cgtccgcgag cgcagtcagc gcccgggccc gctcccccgg
     8821 tttgcgcggc aggaaaccct cgaagcagaa gcggtcgacc gggagcccgc tgaccgcgag
     8881 cgccatgagc acggcgctcg ggccggggac acaggtgacc cggacgccag cctcgaccgc
     8941 cgcggcgacg agtcggtagc cggggtccga gacggacggc ataccggcgt ctgtgaccac
     9001 gagaaccctt gcgccagaga gcaactgctc gaccagctcg ggagtccggg cggcctcgtt
     9061 gtgctcgtga tagctcagga cccgccccga gatggtcacg ccgaggtcgg tggtgaggcg
     9121 ccgcaaccgg cgggtgtcct cggccgcgac gacgtcggcg ccggccaact cctgtgccaa
     9181 ccggggtgcg gcgtcgcggg ggtcgccgat cggcgtnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnngtgc tcatcgtgct cgggctctcg ctcggcgcgt cggcgatctg
     9361 gtcggtgctg cggatcgtcg aacggctcac ccgggcggtc cctctgggcc agcagacctc
     9421 ctcgctcaac acgtcggtca cgccggaccg gccctggctg gacctggcct accaactgac
     9481 cggcatcggc ctggccctcg tgccggtcgc gttgtgtctc tatctgctgg cgcgagtcga
     9541 gccgcccgag ccggggacgg cggcataccg ggtgctcggt ctggacctgc gccgaccgcg
     9601 cctggacctg ggcggcgggg tcgtgctggc tgcggcgatc ggcatccccg ggctggggct
     9661 ctatctggtg gcgaccaggc tggggttcaa cacccaggtc gcccccgcca acctcgccgg
     9721 agcctggtgg accgtgccgg tgctcgtgct gtcggccctg cagaacgccg tcctcgagga
     9781 ggtcattgtc gtcggctacc tgctcacccg gctcgagcag atgggcgtgg gcgcgcgctg
     9841 ggcggtcggg atctccgcgg tggtgcgcgg gtcctaccac ctctaccagg gcttcggcgg
     9901 gttcgtcggc aacatcgtca tgggggtcgt tttcgccctg gtgtggagac gctggcggcg
     9961 tgtggggcca ctggtcgtcg cgcatgcgct catcgacatc gtcgccttcg tcggctatgc
    10021 cctgctcaag gactacctgc cctgggggtg agcctgcgtc ggcgaaggct tggtcaggga
    10081 gaggcggact cccactgcgg tgcgtcgtag gagcgggggt cctcgatcgg ctccaaatgg
    10141 gtgtcgacga cgatgtggcc gaacttctcg tggaaccggc cctcgatctc ttccaggagg
    10201 tcgtggccct gggtgaccgt ccaggtgccc gggaccagga cgtggaaggc gacgtgctgg
    10261 acgtgcccgg cgacgcgggc ccggaccccg tggaagagga ccggggaccc ttcgcggtga
    10321 gcgaactcgt cgaggatcgc ctggatgtcg gcctggcact ccggcgaggg ggcgtggtcc
    10381 atgagcccgt cgacggactc gcgcacgagg tgccagccgg tccagatgat gttgcagccg
    10441 acgaggaagg cgatgatcgg gtcgaggcgc agccagccgg tcagcacgac gagcaagacg
    10501 ccgacgacga ccccgaccga ggtccagacg tcggtgagca ggtgtttgcc gtcggcggtg
    10561 agagtgatcg agttgtgctt gcgaccggcc cgcatgagga cgaacgccac cacgccgttg
    10621 agcgccgacg ccgccaccga gatccccaga cccatcccga cgttctccag cggctcgggg
    10681 gcgaagaacc gctgcaccga cgaccagagg atgaagatgg cggcgacgaa gatcatcacg
    10741 ccctcgacgg cagccgagaa gtactcggcc ttggagtgac caaactggtg gtccacgtcg
    10801 gcgggttggg cggcgacgtg caaggcgatg agggcgacga aggccgccac gagattgacc
    10861 acggattcgg ccgcgtcgga cagcaatccg accgatccgg tcagcaggta ggccccgaac
    10921 ttcagcgcaa tcgtcgctac ggcggcggcg atcgacagcc aagcgaagcg ggtgaggttc
    10981 tcacgggcag gcgcgggcgg cataccggac agtctggcac tgccgccgga gtgcctacga
    11041 tgtcgctcat gacgagcgag gagcagctgc gccggcggct gctgggggac ccacccccac
    11101 gggacctgct cggctggctc ggcccgctcg tagccatgat cctcggcggg atcatccggt
    11161 tctgggacct cgctcggccc caccagctgg tcttcgacga gacgtactac gtcaagcagg
    11221 cctggtcgat gctcgagtgg ggcgtcgaga tgcgcaacag ctcggccttc ggcgacaagc
    11281 ccgacgaagc cttcacccac ggcacgccca acgtcttcaa ctcgaccgac ggcgatctgg
    11341 tcgtccacgg cccagtcggc aagtgggtca tcgcctcggg cgaggcgatc ttcggcggcg
    11401 acagttcgtt cgggtggcgg ttcgcggtct gtgtgctcgg gaccatctcg atcttcatgc
    11461 tcggccggat cgccagacgc ctgctcggtt cgtcggtcct cggcaccatc gcggcggtgc
    11521 tactcgcctt cgagggccac catttcgtcc actcccgcac cgggctgctc gacctcatcc
    11581 tcatgttctt cgcgctcggg gccttcgggg cgctgttgct cgaccgcgac cgatcgcggg
    11641 cggtcctggc gcgccggatg gccgccgcgt cgcccgaccg gctgcgcgac caccggtggc
    11701 tgggcccctc gatgggctgg cgtccgtggc gctgggtggc cggcctgtcc ctcggcctgg
    11761 cagcggggac gaagtggtcg gggctctact tcctcgtcgt cttcgggctg atgacggtct
    11821 tctgggacct cggcgcccgg cgtgccgtgg gggtccgggc ctacggcctg gcgacggtgg
    11881 tgcgggacgg cataccggcg ttcttcacgg tcgtgccgac ggtcgcggtg acctacctgg
    11941 tgtcgtggtg ggggtggttc gtcagcgacc acggctacaa caaggcgtgg gcggccactc
    12001 acccggcctc gtcgacctgg gcgtgggtgc ccgacgacct gcgctcgtgg tggaactacc
    12061 aggcccagat cctcgagttc caccgcggcc tgtcctcgcc gcacagctat cagtccagcc
    12121 cctggtcgtg gctcgtgatg ggccgaccaa cgtcgatgtt cgccgaatac cccaagctcg
    12181 gcgaagacgg ctgcaccgtc gacatgtgcg ccaaggtcat caccccgatc ggcacgccga
    12241 cgatctggtg gctcggggtg gccgcggtgg tcatcctcgt gttccagtgg gcgctgcgtc
    12301 gcgactggcg ggcgggggcc atcctcgcgg ggtatgccgg tggctacctg ccgtggttcc
    12361 tcgtcggcga ccggaccatc ttccagttct atgccgtggc gttcgtcccc tacgtcgtgc
    12421 tggccgtgac ctacgtgctc gggatgatcg ccggacgccg cgacgcgcca tggcggcgac
    12481 ggcagatcgg tgccggagtg gtcggcggat tcctcgtgct gaccgtgctg ctcttcgcct
    12541 ggttctggcc gctctacacc gcacaggtca tcccgtattc acagtggtgg ctgcacatgt
    12601 ggtttccctc ctgggtgtga acgcgcgcca cgggtgagcg aaagcggcat accgggacgt
    12661 atgccgcgcc acgcgccgca gccgtcgcgc gatctcgctg acatgatgag tccatgaccg
    12721 gcaccgcgct caggggggtg tgggtggcga tcatcgccgt cctcgcactc gcaggcgggt
    12781 gtggcctcga cgcccccacg acggtcggcc cgagcacgtc cgtcggcgtc gacccggcgc
    12841 cagcaccgac cgcgccggct gagaacctca ggcccgccgg tcccgggttg gcgctgtcga
    12901 tcctgccggt cgggtccagc gaacaggcga tcaccgtcga cggccggatc cggatgtatc
    12961 gcgcctatcg accggcccga ttggctcgga ccgcgccgct cgtcgtgatg ttccacggcg
    13021 ggctcgggag cgctcggcag gcggagagcg cctacgggtg ggacacgctg gccgaccgcg
    13081 agggcttcgt cgttctctac cccgacggca tcggcgccgc gtggaacgtc ggcggtggct
    13141 gctgcgggct cgcaccagcg cagggggtca acgacgtcgc cttcgtcagg gccgcgatcg
    13201 acaacctccg cactcgcgtc tcgctcgacc ccggccgcac gtatgccgcg gggatgtcgg
    13261 cggggggcat gatggctttt cggatggctt gcgacacaac gctgttcgcg gcgatcgggc
    13321 cggtggccgc cacccagctc ggcgactgcc ccaatccggc gcccacgtcg gtgatgcagg
    13381 tccacgggac cgccgaccca acggtgccgt tcgagggctc ggggacgtcg gcgtcggggt
    13441 cggggtcggc tccggggtca gggttggagc ccgggctgga gccggagccg gggatcgggc
    13501 cgggcatcgc ccagatcgtc gggccggcga tccccaccct gcatgcgacc tggcgcggca
    13561 tcggtcgatg cggtggcgac gtcatcgcct cgattcatgg ggtgacgacc cagacggccg
    13621 agtgccccga gtctcggacg gtcaagttgg tcatcattga aggcggcggt gccgactggc
    13681 cgggagtcgg ccgctccggc ccgctggatg cctcaccgtc agcgaccgca gcgcccacgg
    13741 cgacggtggc tgtgcccacc gtggggccga cagtgtcacc cgatgccgtg gcggcataca
    13801 acacgactgc gcgactgtgg gagttcttca aggcgcacga gcgctgaggc gagacggcga
    13861 aggcgcaagg gggcagcgag ggggcagggc gcggcgctgc ggtcgtgcgg tcgcccggac
    13921 aggatgcgct tagggtgcgc gaggtgacgg gtttcggcca aggagaagga acttcgtctg
    13981 cagtcgctcg ccggtcggtc ccacccacct cgtcgcgggt ggtcgctcct gccgatccgg
    14041 tgccacgcgt cggtcgggag cgaggggtcg gatcggccag ccgccgcgag cccagaacgt
    14101 caggccggtc ggcgtccgtg gtgtgccgct gatcgtgaac tcaccctcgt gatggcggtc
    14161 atggtgatag gggcacaggc tgacgagttg gtcgaggtcg gtccggccac cgttgcgcca
    14221 gtggacgatg tggtgggcct cgacgtgccc cgccccgaca caacccgggt agcggcagcc
    14281 ctggtcgcgc gactcgatga ggcggcgggt gcggtcgggg acgacacgct gggatctccc
    14341 gacgctcacc ggcaccccgt cggtctccca gacgggcgcc aggacgccat cgcaggtcat
    14401 tgcgtcgacg atgtgcgacg gcaggcggcc acccttgccg atccagccac cgtcggtgtc
    14461 gaggtggaca tagatgcggt acttcgccga ccgcgacgtc ccctccaccg ccgacaacga
    14521 ccggttggca acctcggcca gcccatcggc cagcgagacg tcgacgaggc cggcgtggaa
    14581 gagggagtcg cgcgcctcgc ggatcgccgt ctcgacgagg gcaccgacgg acgcgggcgc
    14641 cgagtaggac agctcgaatc gtccgtccac cgtcgacatc gacagccggg gccgctcggt
    14701 ctccggtcct ggatgcgggg cctcctcggc ctcttcggtg ttcttggtgt cctcggtcga
    14761 cgcctggtcg ggcgagtcac ccgcctcatc ggcgaacgca tagcgcgcca gtgaccggcg
    14821 caactgcgtc accgtcgcca actccgccaa ctcgctcgcc tgcgcggagt atgccgcggg
    14881 cacataccgc gcgaccactg cggcttgatc gacacccaac cggccgtcag acatggccga
    14941 gagcgtctcg ggcagctcgt cgctgcgtgc ggcgagcagg gcgatctcgc gggctcgact
    15001 cggtgacagc ccggcccgca cgatgagcca gtgctgcggc gagcggatgc cgccctcggc
    15061 ccagagctcg gtctccaaca cctcgcgggt gatctcgacg agctcggcgt gggccctggc
    15121 caaccgggcg cacacggcag cggtgcgagc gtggacgtcc tcgacatccg ccgcttgcac
    15181 caccactgtc gccggttgac tcatacctca atcatacaag tgttcgagtc cttcggcaag
    15241 gtgtccggcc atcaattcac ttcgggcaca agggtttcgg acgagattgc ctgcccggcg
    15301 caaacgacga aaccccgccc gaccacaggc gttcctgctg gtcgaacggg gtttccatcg
    15361 agtggagctg aggggattcg aacccctgac cttctcattg cgaacgagac gcgcta
//
LOCUS       DAORPW010000049        15049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_786_length_15049_cov_744.338735, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000049 DAORPW010000000
VERSION     DAORPW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15049
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_786_length_15049_cov_744.338735"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PLB86_05985"
     CDS             complement(<1..579)
                     /locus_tag="PLB86_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRC64719.1"
                     /translation="MTHPSFDVARRLLRRPSERESRHLGDILRTETVGGMLLLGATVV
                     ALAWANSPWHTAYTGLRDTRVGPSAPLHLDLTLGQWAADGLLALFFLVAGIELKREFV
                     SGDLADRTKAIVPVAAALAGVALPAILYAVTVLALGGDSTAMRGWAIPTATDIAFALA
                     VLAVVGTHLPPALRAFLLTLAVVDDLVAITIIA"
     gene            748..1461
                     /locus_tag="PLB86_05990"
     CDS             748..1461
                     /locus_tag="PLB86_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC64720.1"
                     /translation="MTSSADPAALPQCAVAVDLVVLTVREGQLCVLTVRRGIPPFRGR
                     FALPGGFVLPDEGLEAAAYRELAEEAGLAREAVTLEQLRTFGEPKRDPRGRVVSVAYL
                     ALGAGLPDPLGGSDASSAQFEPVSGYLAHPAGPTALAFDHPMILAAGVERARAKLEYT
                     GYGVAFCPPEFTVGELRGVYESVWDLAIDPANFHRKVTNAAGFLVPTGERTSRGGGRP
                     AALYRADPAAVLHPPILRG"
     gene            complement(1463..2041)
                     /locus_tag="PLB86_05995"
     CDS             complement(1463..2041)
                     /locus_tag="PLB86_05995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRC64721.1"
                     /translation="MPTPEAPRTGLIELRIVVASTRPRRLGPVIADWVRNQAPLDVFA
                     VEVIDLAELNLPLLDEVEMPSTGIYHHEHTLRWKGLVDAADAIVLVTPEYNAGYPAAL
                     KNAIDYLYAEWAGKPIALFAYGHSGGASASAQLSVVLSRVKATLVDGVGLRYRDHLDG
                     TVGPEATVRADAELLGATRRMYAALAAAARPS"
     gene            complement(2235..4205)
                     /gene="acs"
                     /locus_tag="PLB86_06000"
     CDS             complement(2235..4205)
                     /gene="acs"
                     /locus_tag="PLB86_06000"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRC64722.1"
                     /translation="MSNETLANLLHEDRSFPPDPAFAAQANGTAELYAAADADYEGFW
                     GDQARRYVTWSKPFTEVLDWSNAPFAKWFADGELNAAYNCVDRHVEEGRGDKVALHWV
                     GEPGDTRDVTYADLHREVQQAANALIDLGLGKGDTVAIYLPMIPEAVVAMLACARIGV
                     PHSVVFGGFSAEALNTRIADAQAKVVITSDGGYRRGTPSALKPAVDAALDHADTSVQK
                     VLVVRRTGQDIAWNDGRDLWWHEAMAAANPVHVAQGMEAEHPLFILYTSGTTGKPKGI
                     FHTTGGYLTQAAYTNAVVHDVHPETDVYWCTADIGWVTGHSYIVYGPLALGATQVLYE
                     GTPDTPHQGRFWEIVQQYKVSILYTAPTAIRTFMKWGSDIPAKFDLSSIRMLGSVGEP
                     INPEAWMWYRNVIGGGHAPIVDTWWQTETGGIMISPLPGVTNLKPGSAQRPIPGIGAE
                     VVDDMGEPVPDGHGGYLVLTKPWPAMLRGLWGDPARYVETYWSRWPQYYFAGDGAKKD
                     EEGNIWLLGRVDDVMNVSGHRLSTTEIESALVSHPKVAEAAVVGANDEMTGQAVVAFV
                     ILRAGSSHADDDNTGIVEELRNHVAKEIGPIAKPKRILIVPELPKTRSGKIMRRLLRD
                     VAEGRQVGDATTLADTSVMDLIKTGLSSGSDG"
     gene            complement(4332..4634)
                     /locus_tag="PLB86_06005"
     CDS             complement(4332..4634)
                     /locus_tag="PLB86_06005"
                     /inference="COORDINATES: protein motif:HMM:NF023257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HRC64723.1"
                     /translation="MGGLKMQSLRGWLVEGRPRGVARADLLAPALLRCELTEAETSAV
                     VGALRVRAGHSVADPVPDDDIRRVVQALVYEGADEAEVYRVREAMRRAGDRVTSHT"
     gene            complement(4953..5984)
                     /gene="trpS"
                     /locus_tag="PLB86_06010"
     CDS             complement(4953..5984)
                     /gene="trpS"
                     /locus_tag="PLB86_06010"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRC64724.1"
                     /translation="MTTQPSGIPPRSRPRSLSGMQPTSDSLHIGNYVGALVNWVELQE
                     TYDAFYFVADLHALTVPTGPAVLRRRTQVTAAQFIAAGVDPERSAVFCQSHLPQHAEL
                     GWVMSCLTAMGEASRMTQFKDKTAKGQQGNVGLFTYPMLMAADILIYDADKVPVGEDQ
                     RQHLEITRDLALRFNQRYGQTLVVPEPHILKQTAKIMELTDPTSKMSGTNSPDAGVVL
                     LSDDPKRITKKIKSAVTDAEMEIRYDPENKPGVSNLLVIQSALTGVPIPALEAGYAGQ
                     GYGALKTQVAEVVVAAAEPFQRRMAELLDDPAELDRVLARGAERAGAVSAATLARVKD
                     RMGLLPAAH"
     gene            complement(6242..6583)
                     /locus_tag="PLB86_06015"
     CDS             complement(6242..6583)
                     /locus_tag="PLB86_06015"
                     /inference="COORDINATES: protein motif:HMM:NF023257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HRC64725.1"
                     /translation="MTSSPSIAARILDWLRGGYPEGVPSGDYIALLGILHRRLTTAEV
                     QALAGDLAAEAADSPPLTAEDVRALIEDRIHEDADDESVARVSARLAAGGWPLAPLRD
                     GDSLPPAPRQP"
     gene            complement(6592..6888)
                     /locus_tag="PLB86_06020"
     CDS             complement(6592..6888)
                     /locus_tag="PLB86_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64726.1"
                     /translation="MNNIGLAFDGAWKVLVAGVLLGAGLPAIFALGIRALAYGAGGDA
                     EVDHEAGHPIGKVLAILCFALVVIGIALGITYIVATGFGKTLSFQNFYPTLVSK"
     gene            complement(6901..8127)
                     /locus_tag="PLB86_06025"
     CDS             complement(6901..8127)
                     /locus_tag="PLB86_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRC64727.1"
                     /translation="MTTATVILGLVVATALAFDFTNGFHDTGNAMATSIATGALRPKA
                     AVLLSAVLNLVGAFLSVQVALTVTNAVVKIQDKSGAPSAALLADGGYALLLIVLAGLC
                     GGIVWNLLTWLLGLPSSSSHALFGGLVGSAIAGLGLSGVNWVGDGSKLDGVVGKVIMP
                     ALLSPVIAVVVSTIGTWLIYKITAGVAQRFQEAGFRWGQIGSASLVSLAHGTNDAQKT
                     MGVITLALIGAGQWSDLTAVPLWVKVTCAVAIALGTYLGGWRIIRTLGKGLVEISSPQ
                     GMAAESSSAAVILASSQMGFALSTTHVATGSILGTGLGRRAEVRWGVAARMLVAWTIT
                     LPAAALVGAAMWFIGHLLGGLLGAVVVFALLVGLAAYMWVRSRRAPVDHTNVNDAWDT
                     DASKLSETLATSPARI"
     gene            complement(8378..9958)
                     /locus_tag="PLB86_06030"
     CDS             complement(8378..9958)
                     /locus_tag="PLB86_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRC64728.1"
                     /translation="MPAATQVGNTTLNIAIFGLFVLITLGIVIRVSKQNTTAADYYAG
                     GRAFSGRQNGIAITGDYLSAASFLGIAGAIAINGYDGFLYSIGFLVAWLVALLLVAEL
                     LRNTGRFTMADVLSFRLRQRPVRTAAAVNTLAVSAFYLLAQMAGAGGLVALLLGITDR
                     TGQSIVIAIVGALMIVYVLIGGMKGTTWVQIIKAALLILGAGIMTIWVGVKYGFNISA
                     LFDDAIKQAGNAAILNPGLQYKNKIDFISLSIALVLGTAGLPHVLMRFYTVPTSKEAR
                     RSVVWAIWLIGLFYLFTLVLGYGAQALVGSAAIKAAPGGVNSAAPLLAYHLGGELLLG
                     VISAVAFATILAVVAGLTITASASFAHDIYAQVIKRGTVSPDDEVKVARRTAVVIGAL
                     AIVGGIFANGQNIAFLVALAFAVAASANLPTILYSLFWKNFTTRGALMSMYGGLASSL
                     LLIIFSPVVSGSATSMFPTASFAWFPLNNPGIISVPLSFFLGWLGSVTEKEHNAAKYA
                     EMEVRSLTGAGAEKAVSH"
     gene            complement(9970..10314)
                     /locus_tag="PLB86_06035"
     CDS             complement(9970..10314)
                     /locus_tag="PLB86_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HRC64729.1"
                     /translation="MSSHNDISEERAARYIAVQNSQEFAELRKTFRKFVFPMTGLFLA
                     WYFLYVILATYASDFMATKLFGNITVGLVLGLAQFATTFAITMIYARWADRVLDPAAA
                     KLADHMERGNLQ"
     gene            complement(10469..12025)
                     /locus_tag="PLB86_06040"
     CDS             complement(10469..12025)
                     /locus_tag="PLB86_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRC64730.1"
                     /translation="MNTLASGWGLFAIILVCVATVAVGALGLRMSRTTSDFYVAGRVV
                     TAGWNASAIGGEYLSAASFLGVAGIIYSLGTHMLWLPVGYTLGYLILLVLVAAPLRRS
                     GAYTIPDFAEARLSSRRVRRIASVLVVGIGWLYLLPQFQGAGLALQTVTGASPAVGSL
                     IVAVVVLVSVAAGGMRSITLVQAMQYWLKLAAISIPCFVLAWLWFRGGQPLPSLTDPG
                     GPFAGALPAYPMREHPLYATYSTLLALCFGTMGLPHVLVRFYTNPDGPAARRTTVIVI
                     ALLGAFYLFPPVLGMLGRIYLPSLPAGTRADSVVLLLPSAVTPGTLGDLLSAILAGGA
                     FAAFLSTASGLTVSVAGVIDQDLLSGAMNRRLGGDLPRTHSFRLASLVAILIPYAASW
                     LAPGATLADTVGMAFAIAASTLGPLLILGVWWRRLSVTGAAAGLIVGGTLAMGATVAT
                     LFGVGRGTGGWIPVLLAQPAAWSMPIAFTTAVLVSLATPRSVPVNAARAMVRLHAPEE
                     VTARVRPPAG"
     gene            complement(12036..12464)
                     /locus_tag="PLB86_06045"
     CDS             complement(12036..12464)
                     /locus_tag="PLB86_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64731.1"
                     /translation="MTPTAGPPDPRGDRSLPEVGPAPKRVRVVSPRRDARRRKEKRPD
                     AVEFTEQTGLGEIYLTALLRAQLRLSLAILVAVGGILVGLPALFLLVPGAAAATLGPV
                     PVIWLVAGVAVYPIVLLAARLHVRMAERIERDFTDLMAGR"
     gene            complement(12469..13233)
                     /locus_tag="PLB86_06050"
     CDS             complement(12469..13233)
                     /locus_tag="PLB86_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRC64732.1"
                     /translation="MTTTTSSPPLRALLVDDEAPTLAELAWLLGQDERIGEIYTAGSG
                     TEALKALDDHPVDVIFTDISMPGLDGLALAKVLARFADRPQVVFVTAYDEHAVTAFEL
                     EVTDYVMKPVRADRLGEAVRRVVAQRAEKRVTVVPVPDAPADETIPVELGGVTTFVQR
                     SQILYAQAQGDYARLHTDRGSHLVRISLNALEERWGDHGFVRIHRSTLVSTAHITAVT
                     VQSGRCKVSLGDDIALEVSRRHTRALRDRLIRSAAR"
     gene            13327..13917
                     /locus_tag="PLB86_06055"
     CDS             13327..13917
                     /locus_tag="PLB86_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC64733.1"
                     /translation="MKRSYRQSERARQAEETGVRILQETRRLFEAQGYAGLTLASVAE
                     AAGVTTQTVIRRFGDKEGLTAAAGQRVAQEIYAQRDEAPAGDLAAIVRNLVEHYETRG
                     PVSLRLLADEHLSEAVASFTRAGRDFHHGWCERVFAPWLADLDGSTSRRRVAQLVAIC
                     DVRTWELLRHREGLTPAQTRTALLELLRPLTEGARQ"
     gene            13914..>15049
                     /locus_tag="PLB86_06060"
     CDS             13914..>15049
                     /locus_tag="PLB86_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC64734.1"
                     /translation="MTAVLAYTSPAMGHLYPMMPLLLELRDRGLDVHVRTLDTLVDQA
                     RAAGLSAERIDARLRPIADKHPDWAATNAKAALERSATMFAERAVLDGPDFRRAIEEV
                     DPDLLIVDTNAWGALVVAEAQRRPWLHFSPYLVPLRSTGTPPFGPGLPRWDNPVGRVR
                     DAVVGRLVFGAVERSIIPRINALRTPAGLAPVRSFDDLVLAAPLVAICSATPFDYPHP
                     DWPSHVISIGAMTWEPPTAPPAWLADLPDPLTLVTTSSEYQADEALARVAVAALASEP
                     GSLVVTMPAGVPDDLRDLPPNVRVEQFVPHAPLVARAAVAVTHGGMGATQKALAGGIP
                     VCVVPYGRDQLETAARVVHADAGTQLAPKKLTPAALRAAVRAAAG"
BASE COUNT         2346 a         4829 c         5656 g         2218 t
ORIGIN      
        1 cgcgatgatg gtgatcgcga cgaggtcgtc gacgacggcc agggtgagca ggaacgccct
       61 cagggccggc ggcagatggg tcccgacgac ggccagcact gcgagggcga aggcgatgtc
      121 ggtcgcggtc gggatcgccc agccgcgcat cgcggtgctg tcgccgccga gcgcgaggac
      181 ggtgacggca tacaggatcg cggggagggc cacccctgcg agggcggcgg cgacggggac
      241 gatcgccttg gtgcggtcgg caaggtcacc gctgacgaac tcccgtttga gctcgattcc
      301 cgcgacgagg aagaagaggg cgagcaggcc gtcggccgcc cactgcccga gggtgagatc
      361 caggtgcagg ggggcggagg gaccgacgcg ggtgtcccgc aggccggtgt atgccgtgtg
      421 ccacggcgag ttcgcccagg ccagggccac gacggtggca ccgagcagga gcatgccgcc
      481 gacggtctcg gtgcgcagga tgtcgccgag gtgtcgggat tcccgttcgc tgggccggcg
      541 taggaggcgg cgggcgacgt cgaaggaagg atgtgtcatg gcgcctttcg ggtggtgcgg
      601 gacccgatga gcggcgtcga agcgacgcgc ggtgcagccg aaggcgtgcg acacccgggt
      661 cgccgaccag acttcccggc acgccagagc gaccagtcta tcaggagggg gtcgagattt
      721 agtcatcacg acgattagga tcaggtcatg acgagctcgg cggatccggc ggccttgccg
      781 cagtgcgcag ttgcagtgga cctggtcgtc ctcaccgtgc gcgaaggcca gttgtgcgtg
      841 ctgacggtgc gacgcggcat acctccgttt cgggggaggt tcgcgctgcc gggtggtttc
      901 gtcctgcccg acgaagggtt ggaggcggcg gcctaccggg agttggcgga ggaggccggc
      961 ttggcgcggg aggcggtgac cttggagcag ttgcggacgt tcggagagcc caagcgtgac
     1021 ccgcgaggac gggtcgtcag cgtcgcctat ctcgcgctcg gcgcggggct gcccgacccg
     1081 ctgggcggct ccgacgcatc atccgctcag ttcgagccgg tgagtggcta tctcgcgcac
     1141 cccgcggggc cgacggcgct cgcgttcgac cacccgatga tcctcgcggc cggcgtcgaa
     1201 cgggcccggg ccaagctcga atacaccggt tatggggtgg cgttctgtcc gccggagttc
     1261 accgtcggcg agttgcgcgg ggtgtacgag tcggtgtggg acctggcgat cgacccggcg
     1321 aacttccacc ggaaggtgac gaacgcggcc ggctttctcg tgccgaccgg ggagcggacc
     1381 agcaggggag gcggccgacc ggcagcgctc tatcgggccg acccggcagc ggtgctgcac
     1441 ccaccgatcc tgcggggata ggtcagctgg ggcgagcggc ggcggccagg gcggcataca
     1501 tgcggcgggt ggccccgagc agctctgcgt cggcgcgcac cgtggcctcc gggcccactg
     1561 tgccgtcgag gtggtcgcgg taacgcagcc cgaccccgtc gacgagcgtg gccttcaccc
     1621 tgctgagcac gacgctcagc tgcgcgctgg ccgaggcgcc gccgctatgg ccgtaggcga
     1681 agagcgctat cggcttgccc gcccattcgg cgtagaggta gtcgatggcg ttcttcaggg
     1741 cggccggata gcccgcgttg tattccgggg tgaccaggac gatggcgtcg gccgcgtcga
     1801 cgaggccctt ccagcgcagg gtgtgctcgt ggtggtagat gcccgtcgag ggcatctcga
     1861 cctcgtcgag caggggcagg ttgagctcgg ccaggtcgat gacctcgacg gcgaagacgt
     1921 cgagcggggc ctggttgcgc acccagtcgg cgatgaccgg gccgaggcgg cggggccggg
     1981 tgctcgcgac gacgatccgc agctcgatga gtccggtgcg gggcgcttca ggggtgggca
     2041 tgaccgcatc caagcagaac cccggcgagt gtgagggtct ggggtcgttt ggggggtcgg
     2101 gagccggaat tcaccggcga gtgtgaggat ttgggggaac agcgggggtg tgcggctggg
     2161 cgatggggac acgccagagg gggtatgccg tgggcgcggc ataccccctc cgggcggggc
     2221 aggtggtgcg ggggtcagcc gtccgaaccg gaggacagac cggtcttgat gaggtccatc
     2281 accgacgtgt cggccagggt cgtggcatcg ccgacctgac gaccctcggc cacgtcgcgc
     2341 agcaggcggc gcatgatctt gccggagcgg gtcttgggca actcgggaac gatgaggatg
     2401 cgcttgggct tggcgatcgg gccgatctcc ttggccacgt ggttgcgcaa ctcctcgacg
     2461 atgccggtgt tgtcgtcgtc ggcgtggctg gagccggcgc gcaggatgac gaaggcgacg
     2521 acggcctgac cggtcatttc gtcgttggcg ccgacgacgg cggcctcggc gaccttcggg
     2581 tggctgacca gggccgactc gatctcggtc gtcgacaggc ggtggcccga gacgttcatg
     2641 acgtcgtcga cccggccgag gagccagatg ttgccctcct cgtccttctt ggccccgtcg
     2701 ccggcgaagt agtactgcgg ccagcgcgac cagtaggtct cgacgtagcg ggccgggtca
     2761 ccccagaggc cgcggagcat ggccggccag ggcttggtga gcacgaggta accgccgtgc
     2821 ccgtccggca ccggctcgcc catgtcgtcg acgacctcgg cgccgatgcc ggggatgggc
     2881 cgctgcgccg acccgggctt gaggttggtc acgcccggca gcgggctgat catgatgccg
     2941 ccggtctcgg tctgccacca ggtgtcgacg atcggggcgt gaccgccacc gatgacgttg
     3001 cggtaccaca tccacgcctc ggggttgatc ggctcaccga ccgagccgag catccggatc
     3061 gaggacaggt cgaacttcgc ggggatgtcc gagccccact tcatgaaggt ccggatggcc
     3121 gtcggggcgg tgtagaggat gctcaccttg tactgctgga cgatctccca gaaccgaccc
     3181 tggtgcgggg tgtccggcgt gccttcgtag agcacctggg tggcgccgag cgccagcggc
     3241 ccgtagacga tgtaggagtg accggtcacc caaccgatgt cggcggtgca ccagtagacg
     3301 tcggtctccg ggtgcacgtc gtgcacgacg gcgttggtgt atgccgcctg ggtgaggtag
     3361 ccgcccgtgg tgtggaagat gcccttcggc ttccccgtgg tgcccgaggt gtagaggatg
     3421 aacagcgggt gctcggcctc catgccctgc gcgacgtgga ccgggttggc cgcggccatc
     3481 gcctcgtgcc accacaggtc gcggccgtcg ttccaggcaa tgtcctggcc ggtgcggcgc
     3541 acgacgagga ccttctgcac ggaggtgtcg gcgtggtcca gcgcggcgtc gacggccggc
     3601 ttgagggcgc tgggcgtccc gcgccggtag ccaccatcag aggtgatgac caccttggcc
     3661 tgggcgtcgg cgatccgggt gttgagcgcc tccgcggaga acccaccgaa aacgaccgag
     3721 tggggaacgc cgatccgggc gcaggcgagc atcgccacga cggcctcggg gatcatcggc
     3781 aggtagatgg cgacggtgtc gcccttgccc aagccaaggt cgatcagcgc gttggcggcc
     3841 tgctgcacct cacggtgcag gtcggcatac gtgacgtcgc gggtgtcccc gggctcgccg
     3901 acccagtgca gcgcgacctt gtccccgcgc ccctcctcga cgtggcggtc cacgcagttg
     3961 tatgccgcgt tgagctcacc atcggcgaac cacttcgcga acggcgcgtt cgaccagtcg
     4021 agcacctcgg tgaacggctt gctccacgtg acgtaacggc gggcctgatc gccccagaac
     4081 ccctcgtagt cggcgtcagc ggcggcatac agctccgcgg tgccgttcgc ctgagcggcg
     4141 aaggcggggt ctggcgggaa gctgcggtcc tcgtggagca ggtttgcgag ggtctcgttc
     4201 gacacggaat ttcctcctgc ggcagggcga cgtgtgacct atgcaactgc caatgccggg
     4261 tggaggcaag tgaactgcac gcgatggggg tcaggcgggc agtttgcgcg gcgagcggtt
     4321 gccgcggttt gtcaggtgtg tgacgtcacc cggtcaccgg cgcgacgcat cgcctcgcga
     4381 acccggtaga cctcggcctc gtcggcccct tcgtacacca gggcctggac gactcgacgg
     4441 atgtcgtcgt cgggcaccgg gtcggcgacg gagtggcccg ctcgcacccg caaggcaccg
     4501 accacggcgc tcgtctcggc ctcggtgagc tcgcagcgca gcagcgccgg ggcgaggagg
     4561 tccgctcgag cgactccgcg cgggcgccct tcaacgagcc acccacgtaa cgactgcatc
     4621 ttcaacccgc ccacggtgcc atcatccgcc ggtcggcgaa gggccggggc cctggcggtc
     4681 gaacttctcc cgacgacaac gcgaacactc ggggttgctc gccctggggg cgactgcgcg
     4741 cccgcagttc tggccgtcga cgctgcccgt gcacgctgcc catcggcgct gtccgtcggc
     4801 gctgtccgtc agagagatat ggcggcccgg catccgtcgc ggaggtggat tgccgggtcg
     4861 tcctcgggat cccgctcccg accccgcacg cggcatactg cgtgggcttg gtatgccgcg
     4921 tgcgcaggtg agcggtccct gctggccccc ggtcagtgcg ccgcgggcag cagacccatc
     4981 cggtccttga cccgcgcgag ggtggccgcc gagaccgcgc cagcccgttc ggccccgcgg
     5041 gcgaggaccc ggtcgagctc ggccgggtcg tcgagcaact cggccatccg gcgctggaac
     5101 ggctctgcgg cggccacgac cacctcggcg acctgggtct tgagcgcgcc atagccctgc
     5161 ccggcatacc ccgcctcgag ggccgggatc ggcaccccgg tgagggccga ctggatgacg
     5221 aggaggttgg agactccggg cttgttctcg gggtcgtagc ggatctccat ctcggcatcg
     5281 gtgacggccg acttgatctt cttggtgatc cgtttggggt catccgacag cagcaccacg
     5341 ccggcatcgg gagagttggt gcccgacatc ttgctcgtcg ggtcggtcaa ctccatgatc
     5401 ttggcggtct gcttgaggat gtgcggctcg ggcacgacga gggtctggcc atagcgctgg
     5461 ttgaagcgca gcgccaggtc acgggtgatc tccaggtgtt ggcgctggtc ctccccgacg
     5521 ggcaccttgt cggcgtcgta gatgaggatg tcggcagcca tgagcatggg gtaggtgaac
     5581 aggccgacgt tgccctgctg ccccttggcc gtcttgtcct tgaactgcgt catccggctg
     5641 gcctcaccca tcgcggtgag acagctcatc acccacccga gctcggcgtg ctgcggcagg
     5701 tggctctggc agaacacagc tgagcgctcg gggtcgacgc cggcggcgat gaactgcgcg
     5761 gccgtcacct gggtgcgccg gcgcagcacg gccgggccgg tcggcacggt gagggcatgc
     5821 aggtcggcga cgaagtagaa cgcgtcgtag gtctcctgca gctcgaccca gttgaccagc
     5881 gcgccgacgt agttgccgat gtgcaggctg tcgctcgtcg gttgcatccc cgagagggat
     5941 cgcggccggg accgcggggg tatgccgctt ggctgggtgg tcatcgtctc tccttggtgc
     6001 ggtccgtccc cggcacgaca aacgccgccc caggggcggc gtttgggtgg ggttgcttcg
     6061 tcgggcgccg ccgctatgcg gtgcgccacc agaccttgag ggtcaacgaa gtcacaaggg
     6121 aaggatagcg cccacctcgc gcaggcggca taccggcggg gtcagcgcgc gaaggaggtt
     6181 ggacgcggca tacctgaagg cgggtgtgac caccgcaacg gcaggtatgc cgcgtccggt
     6241 cttagggctg ccgcggagcc ggtggcagcg agtcgccgtc gcgcaggggt gccagcggcc
     6301 atccgccggc ggcgagccgg gccgagacgc gggcgacaga ctcgtcgtcg gcgtcctcgt
     6361 ggattcggtc ctcgatgagg gctcggacgt cctcggcagt gaggggtggg ctgtctgccg
     6421 cctcggcggc aagatcgccc gcaagtgcct ggacctcggc agtggtgagc cgccggtgca
     6481 ggatgcccag cagggcgatg tagtccccgg acggcactcc ctccggataa cccccgcgaa
     6541 gccagtcgag gatgcgggcc gcgatcgagg gcgaacttgt catgccatca cctacttcga
     6601 gacgagcgtg gggtagaagt tctggaacga cagcgtcttg ccgaagccgg tggcgacgat
     6661 gtaggtgatg cccagcgcga tcccgatgac gacgagggcg aagcagagga tcgccaggac
     6721 cttgccgatg gggtggccgg cctcgtggtc gacctcggcg tcgcctccgg cgccgtaggc
     6781 gagtgcgcga atgccgaggg cgaagatcgc cggtaggccg gcgccgagga ggaccccggc
     6841 gacgaggacc ttccacgcgc cgtcgaaggc caatccgatg ttgttcatgg gtcggtcctt
     6901 tcagatccgg gccggggagg tggccaaggt ctcggagagt ttgctcgcgt cggtgtccca
     6961 cgcgtcgttg acgttggtgt ggtcgaccgg ggcgcgccgt gatcggaccc acatgtaggc
     7021 ggcgagtccg acgaggaggg cgaagacgac gaccgctccg aggagccctc cgaggaggtg
     7081 gccgatgaac cacatggccg cgccgacgag ggcggcagcc gggagggtga tggtccaggc
     7141 gacgagcatc cgcgcggcaa cgccccagcg gacctcggcg cggcgaccga ggccggtgcc
     7201 gaggatggaa ccggtggcga cgtgggtcgt cgagagggcg aagcccatct ggctcgaggc
     7261 cagaatgacc gctgccgaag aactctcggc ggccatgccc tgcggggagg agatctcgac
     7321 caggcccttg ccgagcgtcc ggatgatccg ccacccgccg aggtaggtcc ccagggcgat
     7381 ggcgacggcg cacgtgacct tgacccacaa cggcacggcg gtgaggtcgc tccactggcc
     7441 ggcgccgatg agggcgaggg tgatgacgcc catcgtcttc tgcgcgtcgt tcgtgccgtg
     7501 ggcgagcgag acgagggagg cggagccgat ctggccccag cggaacccgg cttcctggaa
     7561 gcgttgggcg acgcctgcgg tgatcttgta gatgagccac gtgccgatgg tcgacaccac
     7621 caccgcgatg acgggggaga ggagcgcggg catgatcacc ttgcccacga ccccgtcgag
     7681 cttgctgccg tcgccgaccc agttgacgcc ggagaggccc agcccggcga tcgccgagcc
     7741 gacgagcccg ccgaagaggg cgtgggagga cgacgagggc aggccgagca gccaggtcag
     7801 gaggttccag acgatgccgc cgcagaggcc ggccaggacg atgagcagca gcgcatagcc
     7861 gccgtcggcg agcagggccg cgctcggcgc cccgctcttg tcctggatct tgacgactgc
     7921 gttggtcacg gtgagcgcga cctggacgga gaggaaagcg ccgacgaggt tgagcacggc
     7981 gctgaggagg acggcggctt tcgggcggag ggcgcccgtg gcgatcgagg tcgccatggc
     8041 gtttccggtg tcgtggaaac cgttggtgaa gtcgaaggcc agggctgtcg cgacgacgag
     8101 gccgaggatg acggttgcgg tggtcacgct tacatagtgc ggatgccttt gcccgaattg
     8161 tcgggattgg gctctccgat gtccaagtca tcggccactg ttcatgcccc gtatgcgggg
     8221 cctttcagtc aacctcgttc gaacaacaca gcgccccgaa ccttgtcggc ccggggcgct
     8281 gcggctggtg agagaggtat gccgcgcggc tcacgacagt gggagccgca cggcatacct
     8341 cgtgctgtgg tgcggtggtg ctgggtgaag ccggtcgtca gtggctgacg gccttctccg
     8401 cgccggcgcc ggtgagggag cggacctcca tctcggcgta cttcgcggcg ttgtgctcct
     8461 tctcggtgac cgaacccagc cagccgagga agaaggacag ggggaccgag atgatgcccg
     8521 ggttgttgag cgggaaccac gcgaagctcg ccgtggggaa catcgatgtc gccgaacccg
     8581 acacgaccgg gctgaagatg atgagcaaca gcgagctggc caggccgccg tacatcgaca
     8641 tgagggcgcc gcgggtcgtg aagttcttcc agaacagcga gtagaggatc gtcggcaggt
     8701 tggcgctcgc ggcaaccgcg aaggccaggg cgacgaggaa cgcgatgttc tggccgttgg
     8761 cgaagatgcc accgacgatg gcgagggcgc cgatgacgac cgccgtgcgg cgggccacct
     8821 tgacctcgtc gtccgggctc acggtgccac gcttgatgac ctgggcgtag atgtcgtggg
     8881 cgaaggacgc cgatgccgtg atggtgagtc cggccacgac cgcgaggatc gtcgcgaagg
     8941 cgaccgcgga gatgacgccg aggagcaact caccgccgag gtggtaggcc agcagcggag
     9001 cggccgagtt gacgccaccg ggcgcggcct tgatggcggc cgagccgacg agcgcctgcg
     9061 cgccgtaacc gaggacgagg gtgaacaggt agaacaggcc gatgagccag atggcccaga
     9121 cgaccgaacg acgagcttcc ttcgaggtcg gcacggtgta gaagcgcatg agcacgtgcg
     9181 gcagaccggc ggtgccgagg accagggcga tcgacagcga gatgaagtcg atcttgttct
     9241 tgtactgcag gccggggttg aggatggccg cgttgccggc ctgcttgatg gcgtcgtcga
     9301 agagcgccga gatgttgaag ccgtacttca cgccgaccca gatcgtcatg atgccggcgc
     9361 cgagaatgag cagcgcggcc ttgatgatct ggacccaggt ggtgcccttc atcccgccga
     9421 tgaggacgta gacgatcatc agggcgccga cgatggcgat gacgatgctc tgacccgtgc
     9481 ggtcggtgat gccgagcagc agggcgacga ggccaccggc gccggccatc tgggccagca
     9541 ggtagaaggc cgacaccgcg agggtgttga ccgcggcggc ggtgcggacc gggcgctgac
     9601 gcaaccggaa cgacagcacg tcggccatgg tgaaacggcc ggtgttacgc agcaactcgg
     9661 caacgagcag gagcgcgacg agccaggcga cgaggaaccc gatcgagtag aggaacccgt
     9721 cgtagccgtt gatggcgatg gcgccggcga tgccgaggaa ggacgctgcc gacaggtagt
     9781 cgccggtgat ggcgatgccg ttctggcggc ccgagaaggc gcggccaccg gcgtagtagt
     9841 ccgcggcggt cgtgttctgc ttggacaccc ggatgacgat gcccagggtg atgaggacga
     9901 acaggccaaa gatggcgatg ttcagggtcg tgttgccgac ctgggtcgcg gcgggcataa
     9961 ccagggcgtt cactggaggt tgcccctttc catgtggtcg gcgagcttgg cggcggcggg
    10021 gtcaaggacg cggtcggccc agcgggcgta gatcatcgtg atcgcgaacg tcgtcgcgaa
    10081 ctgcgccagg ccgaggacca ggccgacggt gatgttgccg aagagcttcg ttgccatgaa
    10141 gtcgctggcg taggtcgcga ggatgacgta gaggaagtac cacgcgagga agagaccggt
    10201 catggggaac acgaacttgc ggaaggtctt ccgcagctcg gcgaactcct gcgagttctg
    10261 aacggcgatg tacctcgccg cgcgttcctc gctgatgtcg ttatggctgc tcaaggtgta
    10321 aacctccggt gggcaggcct gcgaaagggc tccgcagtgt cgtgggtcac acaatgaggg
    10381 cgagtcacga cggctgacta gacgtcacgc gcccggtggg gtccgcgctc gcccagcggt
    10441 cgggggcacc cgcccagcgg ttggctactc agcccgccgg aggccgcacc cgggcggtca
    10501 cttcttcggg ggcgtgcagg cggaccatcg cgcgagcggc gttgacgggc accgaacgag
    10561 gggtcgccag ggacacgaga accgccgtcg tgaaggcgat cggcatcgac cacgcggccg
    10621 gttgggcgag cagcaccggt atccacccac ccgttccacg gccgacgccg aagagggtcg
    10681 ccacggtggc gcccatcgcc agggttccgc cgacgatcag ccctgccgcc gcgccggtga
    10741 ccgacaaccg gcgccaccac acgccgagga tgagcagggg gccgagggtg gaggccgcga
    10801 tcgcgaaggc catgcccacg gtgtcggcga gggtcgcccc gggagcgagc cacgaggcgg
    10861 catacgggat gaggatggcg accagggacg caagccggaa gctgtgcgtg cgcgggaggt
    10921 ccccgccgag gcgacggttc atcgcgccgg agagcaggtc ctggtcgatg acccccgcaa
    10981 ccgacacggt cagaccgctc gcggtcgaca ggaacgccgc gaacgcgccc ccggcgagga
    11041 tggcgctgag caggtcgccg agggttccgg gggtgacggc ggagggcagc agcagcacga
    11101 ccgagtcggc gcgggtgccg gcgggcaggc tcggcaggta gatgcggccg agcatgccga
    11161 gcaccggggg gaagaggtag aacgcgccga gcagcgcgat gacgatgacc gtcgtgcgcc
    11221 gcgcggccgg cccgtcgggg ttggtgtaga agcgcaccag cacgtgtggc aaccccatgg
    11281 tcccgaagca cagggcgagg agcgtcgagt aggtcgcgta gagggggtgt tcccgcatgg
    11341 ggtatgccgg tagcgcgccg gcgaaggggc cgcccgggtc ggtgagggag ggcagcggct
    11401 gaccgccccg gaaccacaac caggccagga cgaagcacgg gatggagatg gcggcgagct
    11461 tgagccagta ctgcatcgcc tggacgaggg tgatcgagcg catcccgccg gccgcgacgc
    11521 tgacgagcac gacgacggcg acgatgaggc tgccgacggc gggtgaggcg cccgtgacgg
    11581 tctgcaacgc caggccggcc ccctggaact gggggagcag atagagccag ccgatgccga
    11641 ccacgaggac gctcgcgatc cggcggacgc gtcgcgagga caaccgcgcc tcggcgaagt
    11701 cggggatcgt gtaggcgccc gatcgccgca acggcgccgc gacgaggacg aggaggatga
    11761 ggtagcccaa cgtgtatccg accggcagcc acagcatgtg ggtgccgagc gagtagatga
    11821 tgcctgccac cccgagaaag cttgcggcag aaaggtattc gccgccgatg gccgaggcgt
    11881 tccacccggc ggtgacgacc cgtccggcga cgtagaagtc gctcgtcgtc cgggacatcc
    11941 gcagcccgag cgcaccgacg gcgacggtcg ccacgcacac gaggatgatc gcgaagagcc
    12001 cccagccgga ggcgagcgtg ttcatggccg ggtcgtcacc ggcccgccat cagatcggtg
    12061 aagtcgcgct cgatccgttc ggccatccgc acgtgcaggc gagcggcgag caggacgatc
    12121 ggatagacgg ccacgccggc gaccagccag atgacaggga ctggaccgag cgtggctgcc
    12181 gcggcgccgg ggacgaggag gaacagcgcg ggcagcccga cgaggatgcc gccgacggcg
    12241 acgaggatcg ccagtgagag gcgcagttgg gcccgcagca gggcagtcag gtagatctcg
    12301 cccagcccgg tctgctcggt gaactcgacc gcgtcggggc gcttctcctt gcgccgtcgg
    12361 gcgtcccgac ggggtgagac gacgcgcacc cgcttgggcg ccggccccac ctcggggagg
    12421 ctccggtccc cacgtgggtc aggcggccca gccgtggggg tcatggggtc accgtgcggc
    12481 cgagcggatg agccggtcgc gcaaggctcg ggtatgccgc ctgctcacct cgagcgcgat
    12541 gtcgtccccg aggctcacct tgcaccgccc actttgcacg gtgacggcgg tgatgtgggc
    12601 tgtcgagacg agggtcgagc ggtggatgcg cacgaacccg tgatcccccc agcgctcctc
    12661 cagcgcgttg agcgagatcc ggacgaggtg ggagccccgg tcggtgtgca gccgcgcgta
    12721 gtcgccctgc gcctgggcgt agaggatctg gctgcgctgg acgaacgtcg tcaccccgcc
    12781 gagctcgacg gggatggttt cgtcggccgg tgcgtcaggg accgggacga cggtgacccg
    12841 tttctcggcc cgttgcgcga cgacgcgccg cactgcctcg ccgaggcgat cggcgcgcac
    12901 tggcttcatg acgtagtcgg tcacctcgag ctcgaacgcc gtcaccgcat gctcgtcgta
    12961 tgccgtgacg aagacgacct ggggccggtc ggcgaagcgg gcgagcacct tcgccagcgc
    13021 gagtccgtcg aggccgggca tggagatgtc ggtgaagatg acgtcgaccg ggtggtcgtc
    13081 cagggccttg agggcctcgg tgcctgaccc ggcggtgtag atctcgccga tccgttcgtc
    13141 ctgcccgagg agccaggcga gctcggcgag agtgggcgct tcgtcgtcga cgaggagcgc
    13201 ccgcagcggc ggagacgacg tcgtcgtggt catggagcca caggataggc cgctgagccg
    13261 actgacgccg cagaacttct gcttggggga tggcgcgagt ctccttttcg tgtcactatc
    13321 agtaacatga aaaggagcta ccgccagagc gagcgggccc ggcaggcgga ggagaccggg
    13381 gtgcggatcc tgcaggagac gcgccgcctc ttcgaggcgc aggggtatgc cggtctcacc
    13441 ctggcgtcgg tggcggaggc cgcgggggtc accacccaga cggtgatccg gcggttcggt
    13501 gacaaggaag ggctcacggc cgctgccggt cagcgggtgg cgcaggagat ctacgcccag
    13561 cgtgacgaag caccggcggg tgacctggcc gcgatcgtgc gcaacctcgt cgagcactac
    13621 gaaacccgcg ggcccgtctc gttgcggctg ctggccgacg agcacctcag cgaggcggtg
    13681 gcctccttca cccgggccgg gcgcgacttc caccacgggt ggtgcgagcg ggtcttcgcg
    13741 ccgtggctgg ctgacctgga cggctcgacg agccggcggc gggtcgcgca actcgtcgcc
    13801 atctgcgacg ttcgcacctg ggagttgctg cggcaccgcg aagggctcac ccctgcccag
    13861 acccgcacgg ctctgctcga acttctccgc cccctcaccg aaggagcacg ccaatgacgg
    13921 ccgtcctggc ctatacctcg cctgcgatgg gccacctcta cccgatgatg ccgctgctgc
    13981 ttgaacttcg ggatcgcggc ctcgacgtcc atgtccgcac gctcgacacc ctcgtcgacc
    14041 aggcccgagc cgccgggctg agcgcagagc ggatcgacgc gcggctgcgt ccaatcgccg
    14101 acaagcaccc cgattgggcg gccaccaatg ccaaggcggc cctggagcgc agcgccacga
    14161 tgttcgccga gcgggcggtt ctcgacgggc cggacttccg ccgggccatc gaggaggtgg
    14221 accccgatct gctcatcgtc gacacgaacg cctggggcgc cctcgtcgtg gcagaggccc
    14281 aacgccgtcc gtggctgcac ttcagcccct atctcgtgcc cctgcgctcg acagggacgc
    14341 ccccgttcgg ccccgggctg cctcggtggg acaaccccgt gggccgggtg cgggacgcgg
    14401 tcgtcgggcg cctggtcttc ggggccgtcg agcgctcgat catcccgcgg atcaacgcgt
    14461 tgcgaacccc tgccggcctg gcccccgtcc gctccttcga cgacctcgtg ctggctgccc
    14521 cactcgtagc gatctgcagc gcaacgcctt tcgactaccc acaccccgac tggccctcgc
    14581 acgtcatcag catcggcgcg atgacctggg agccgccgac cgccccgccg gcgtggctcg
    14641 ccgaccttcc cgatccgctc acccttgtga cgacctcctc ggagtatcag gccgacgaag
    14701 cgctcgcccg ggtcgccgtc gccgccctgg cctccgagcc gggttcgctc gtcgtcacca
    14761 tgcccgccgg tgtccctgac gacctgcgcg acctgccccc gaatgtccgg gtcgagcagt
    14821 tcgtgccgca tgcccccctg gtggcccggg ccgcggtcgc ggtgacccac ggcgggatgg
    14881 gcgcgaccca gaaggcgctg gcgggcggca taccggtgtg cgttgtcccc tatggccgcg
    14941 accaactgga gacggcagcg cgggtcgtcc acgccgatgc tggcacccag ctggcgccga
    15001 agaagctcac gccggcggcc ctacgtgcgg ccgtgcgcgc tgccgccgg
//
LOCUS       DAORPW010000050        14541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_825_length_14541_cov_982.073243, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000050 DAORPW010000000
VERSION     DAORPW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_825_length_14541_cov_982.073243"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLB86_06065"
     CDS             complement(<1..490)
                     /locus_tag="PLB86_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC64735.1"
                     /translation="MKHIPRRALLAASAAVLLGLAACGGGGSAASTDTAPIKVGIIAD
                     LTGATGDVGKPYNEGMMAYIDWLNAKGGIKGRKIEAMSNDYAYQVPKAEELYKKYVSD
                     GAVVIQGWGTGDSEALRTKVSADKLPFMSASYAEVLTDPKQAAYNFVVAPTYSDQMRV
                     ALN"
     gene            complement(614..1660)
                     /locus_tag="PLB86_06070"
     CDS             complement(614..1660)
                     /locus_tag="PLB86_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC64736.1"
                     /translation="MATATGIHHRTYRAELRLRHTKAEWFRLALVIVLLLVVPYALNT
                     YWLGIANSILIAVVGAVGLNILVGFTGQISLGQGGFLAVGAYTSAILSTRLGLPVPLA
                     IIVAILVTAAIGALFGLPALRLKGLYLAIATLASQEIIMFVVRRWGFLTEDKGYIEVP
                     RLPFIERETFELQWYWILLPITFVAVLMARNVFRTSLGRSFMAVRDQDIAAEAIGVNI
                     TRAKVTAFALSSGFVGLSGALSAYYTETVTWERFTLDVSVLYLAMIIVGGLGSIAGSV
                     YGAIFMMLLPALLTEVGQKAGDSIPFITDQLPAIKNAIFGIAIITFLLIEPRGLDRIW
                     TRMKDYVRFWPFRY"
     gene            complement(1664..2536)
                     /locus_tag="PLB86_06075"
     CDS             complement(1664..2536)
                     /locus_tag="PLB86_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC64737.1"
                     /translation="MSNFLLLTVYGLADGAILALAALGFVLIYKATRVINFAQGEFLL
                     FGAYMFYTAFVVMRLPWILASVVGVIGAIILGVLVERFVLRPLVGESAISVIMVTIGL
                     SSVLKALVQMFFGTSVREQPKILPTGSIEFLGATMPVNRLLVILIAAVVLAAFTIFFR
                     RSRHGIAMRAVADDQQAALTMGISVRQIFALAWALAAVSALVAGVLLADLSAVEQNIA
                     SFGLIVFPVVILGGLDSIPGTIVGGLTIGLLKQYVSGYADPGLAEVIPYVVLVAILMV
                     RPYGIFGETRIERV"
     gene            complement(2533..4542)
                     /locus_tag="PLB86_06080"
     CDS             complement(2533..4542)
                     /locus_tag="PLB86_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC64738.1"
                     /translation="MSASDATFPKLLRGLATKNPDQTALQEKRYGIWQPMSWSTYAAR
                     VQDFAHGLAALGVERGQIVAVLGDNRPEWLIAELAAQSIGAAVVGIYPTSIGEELLHI
                     LTLAQVKVVVAEDQEQVDKLLKLRAEAAAAAVDPSAATSAPFADMPPLLVDTVVFYDP
                     HGLEQYTDECLRDFTAVEALGREWGATHPGWLDEQVARGTSSDIAVICTTSGTTSRPK
                     LAELSHDNLLAMADHLTAIDPIDSNDRYVSFLPFAWIGEQMLAVACGLSRGLTLSFPE
                     DSATQKSDMREIGPDVMFSPPRIWEGMLSEVQVRIDEAGWLKRKVFGWGYDVGDKAAG
                     RRVKGQGAGALGALHWLADQVALRPVRDQLGLARNQHAYTGGAPLGPDVFRFFHAIGV
                     NLKQIYGQTEICGIAVVHRDDDIAFNTVGTPIAGTEIQIADNGEILLRSASVFKGYHR
                     NPAATAEAVDPEGWLHTGDAGYLDDNGHLVVIDRQKDVLTTADGTRFSSAFIENKLKF
                     SPYVEEAVTFAADNGMTAIITIDPMTTGSWAEHERLSYTTYTDLAAKAEVQDLVAEEI
                     HRANEDLPESIRVQRFVLLHKQFDPDDDEITRTRKVRRNVIQDRYGLIIDALNRGDER
                     IGITTTVTYQDGTKVDRTIDLRIFDLTTYRVPEGRGRRPVWSGRR"
     gene            complement(4542..5381)
                     /locus_tag="PLB86_06085"
     CDS             complement(4542..5381)
                     /locus_tag="PLB86_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC64739.1"
                     /translation="MLGRAYVHSLNSSTVPVGSPILEISDIELRFGGVVALHDVGFTV
                     TQGHIHAVIGPNGAGKSSLLNCVSGLYHPQKGKAVLHTGDGAVTHELTKLPPHRIARL
                     GVARSFQNIELFSHLTVLENLMLGRHTHMKHGVLQSLLWFGPARNQELSHRGIVEEVI
                     DLLSLQPFRHKPVGSLAYGIQKRVELGRALVLQPSLLLLDEPMAGMNSEEKEDMARYV
                     LDVHELAGVTVILIEHDMAVVMDISDRVSVLDFGRLIADGTPDEVKANSAVIEAYLGA
                     EEH"
     gene            5544..6308
                     /locus_tag="PLB86_06090"
     CDS             5544..6308
                     /locus_tag="PLB86_06090"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC64740.1"
                     /translation="MPPLSDALQEAPGSLGTVRNAALLLQLLAEGPAFQQLTDLADRS
                     GLSLATVHRLLRSLVLADLVEQDPQSSRYGLGPELTRLSHRRLARLPVLGALAPYLGQ
                     VRDAVGGTVRVDVVVRTAVVSIDRIDGSGAGVGLYRDPHGPRLALDTVAGRLLLSRAD
                     DERWKQALAEVDEPTRALAEHERDDWAKAAWLRADGPFPGAPPEVGVPVINAAGMPLA
                     TLVADLPLTASPEQIEAAVAHLTRAVAAAVRTLGHG"
     gene            6301..8163
                     /locus_tag="PLB86_06095"
     CDS             6301..8163
                     /locus_tag="PLB86_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC64741.1"
                     /translation="MADGIPEVGLRASAEPDAAAWEPVVAALPWSRPPDRLWDDGPRH
                     GTWFPGGRINAATACISDHARRTPEHVALLWEGEPGDRRELTYAALDADVTALARALR
                     GLGLGVGDVVALHLGWMPETVVAMFACARIGAVFAVVPTPLPPEALADRLRAIGARLL
                     FTQDGAWRRGTVLPLKARADEALSALGGIEHTIVVRRTGIDVGWYEGDRWYHDLLVGV
                     RPGLRREPSEADTPADLPADHPLQVVSLANRRGQPLTVALGTGHTLAIARALHHAGIA
                     DGSILWCAGENSWLGTQVHGVYGPLVAGHTAVLYEGTVDVPTHARAWELMRRHGVTTL
                     VTTPSVLRAMRSWSFELAIGDEPIGAVPQLRRVVAFGEPMDAEVREWAATGLAGHPVS
                     VADGWGQVELGGIVLLDNPIDASAMPDLGRVLVDRSGEVAPDGMPGELVLTRPWAGTL
                     VGVSGPRETLDDAHWSRLPGSYTTGDVARSEPDGGLEFLGRTDEVTSVSGQLVSLSEV
                     RDVLLEHPFVSAADVVERRDQLRGRVIAAAVVLTPDGVASDPTLVARDLGRSVRESLG
                     GLARPRVLMLVDRFGDELRGVERRRALAGIPVPEGPEPRRVTWEQLLVAADHLT"
     assembly_gap    8201..8300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8304..9158)
                     /locus_tag="PLB86_06100"
     CDS             complement(8304..9158)
                     /locus_tag="PLB86_06100"
                     /inference="COORDINATES: protein motif:HMM:NF025580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HRC64742.1"
                     /translation="MSTVPYDPAAAFPIGAPEGWRADPNLADTERFWDGLHWTDETRP
                     SVRPGTYARTQQALAEGGRSGSRALGGVVQGLLTLCGLLAAALLLYTIIVFNALSVWR
                     LQPTPAAEQEVDKLLLLSTLATWADLGLRTITGILFLVWLGIRYTDTRVDHRVLRRST
                     GVAIFSWIIPVLQLWWPAQAVKDLWHASRPDAARLGGVGARLPVPSVFFVWWPAWLLA
                     GSGSTAVMTVLAQPQFELTWLIWGTIGTGVQQLATLIAAWALIVIINQVEDHLVADDP
                     TAAFDGNI"
     gene            9243..9785
                     /locus_tag="PLB86_06105"
     CDS             9243..9785
                     /locus_tag="PLB86_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRC64743.1"
                     /translation="MPPAPAPLGTLDWRPLADHLDLVAGPVAAAAGLVPDALVAPIDA
                     TLADTAAFCAAYQVPAEASANCVVVEGRRAERVVRAAVMVLAVDRADVNKVVRKHLDV
                     RKLSFMGSEAAQEATGMIQGGITPVGLPSDWLILVDSAVVAAGPVVIGGGVRGAKLLL
                     HGGDLAALPGAAVLDLAVPR"
     gene            9826..11571
                     /gene="argS"
                     /locus_tag="PLB86_06110"
     CDS             9826..11571
                     /gene="argS"
                     /locus_tag="PLB86_06110"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRC64744.1"
                     /translation="MPSPHDTLAPLVSAAIATAFGDELRGADPVLRLSQFADLQANFA
                     LPLAKRLGMPPREVATRVLAALDLTGVCSGIEVSGPGFVNLTLDDSWIASSVQAVGAD
                     PMLGVTGDAPRVVVIDYSAPNVAKEMHVGHLRTTVVGDALARTLEHLGHRVIRQNHVG
                     DWGTPFGMLVEHLVEVGEGSSEALVVETDPNAFYLAARTKFDAEGSFADRARARVVAL
                     QAGDPETLRLWGELVGLSKSYFNRIYAALEVTLTDTDLAGESTYNDALAGICEELEAK
                     GIATISDGALCVFLPEFTGREGKPVPLIVRKSDGGYGYGTTDLATIKHRVEDLGADRV
                     LYVVGAPQSLHFQMVFATARLAGWLPDTVEVVHVQIGNVLGDDRKILRTREGKALRLG
                     ALIEESVDAAQAVLDEARPDLDPATRHTLAREIGVGAIKYADLSVAHDSEYVFDLARM
                     VSLTGNTGPYLQYAAARVRSIFRSAGVEIEVPVGGAQRRIVVTEPAERALALALLEFG
                     SVVRGVGAELEPHRLCGYLFDLAQTFSAFYEACPVLKAPDAETRESRLGLCATTFRVM
                     EQGLALLGMATPAQM"
     gene            complement(11737..12111)
                     /locus_tag="PLB86_06115"
     CDS             complement(11737..12111)
                     /locus_tag="PLB86_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HRC64745.1"
                     /translation="MEPVTHLAALGDSLLATARDAESGRAASTLFAAPQLRAVVIALK
                     EGAALAEHNAPPAATLQCLSGQARLWATGREWVLDAGGYAAIPPERHGVDALTDCVLL
                     LTVSGTRAPEPVEVTLEASAPA"
     gene            12260..13216
                     /locus_tag="PLB86_06120"
     CDS             12260..13216
                     /locus_tag="PLB86_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRC64746.1"
                     /translation="MPAITVPDLSVLPSIPAPGPAATQRPVVSRTTAPHGLEGEGFPV
                     KRAFAGVDLSLLDPFIMMDEMGEVDYGPGEPKGTPWHPHRGFETVTYIIDGTMDHADS
                     YGGGGTITNGATQWMTAGSGILHIEAPPEQLVTSGGLFHGLQLWVNLPRTDKLATPRY
                     QDLAATDVALLCSPDGGALVRVIAGDLAGHRGPGDTHTPITMVHATLAPGARLDLPWR
                     RDFNALVYVMGGAGTVGPADAPLRAGQLAVLGTGDALTVSADPTQESRYAAGLDVVVL
                     GGAPIREPIAWAGPFVMNTRAEVVSAFEDYQAGRLGHPLPHA"
     gene            13433..>14541
                     /locus_tag="PLB86_06125"
     CDS             13433..>14541
                     /locus_tag="PLB86_06125"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HRC64747.1"
                     /translation="MLIGVPRESRPGETRVAATPKTVEQLLKLGYQVAVEAGAGAAAS
                     FDDAAYAAAGATVAHPATVWGADIVTKINPPSDEEIALLRQGAILVSLAQPALNPALV
                     DKLAAAGVTAMAMDAVPRISRAQALDVLSSMANIAGYRAVIEAAHEFGSFFTGQVTAA
                     GKVPPAKVFVSGAGVAGLAAIGTANSLGAIVRATDVRPEVGEQVESMGAEFVKPDVPE
                     QEVSSDGYAKEVGADYAAAAAVMYAAQAKDCDIIVTTALIPGRPAPRLITEEMVAAMK
                     PGSVIVDMAASNGGNVAGSVADKMVVTENGVKIIGYTDLPGRLPTQASQLYGTNVVNL
                     MKLITPGKDGVAVLDWEDVIVRGMTVAKEGAVTWPP"
BASE COUNT         2193 a         4839 c         5208 g         2201 t
ORIGIN      
        1 agttgagcgc gacgcgcatc tggtcggagt aggtcggggc gacgacgaag ttgtatgccg
       61 cctgcttggg gtcggtcagc acctcggcgt aggaggccga catgaagggc aacttgtcgg
      121 cagagacctt ggtgcgcagc gcctcagagt cgccggtgcc ccagccctgg atgaccacgg
      181 cgccgtccga gacgtacttc ttgtagagct cctcggcctt gggcacctgg taggcgtagt
      241 cgttggacat cgcctcgatc ttgcggccct tgatgccgcc cttggcgttg agccagtcga
      301 tgtaggccat catgccctcg ttgtagggct tgccgacgtc gccggtcgct cccgtcaggt
      361 cggcgatgat gccgaccttg atcggcgcgg tgtccgtgga ggccgcgctg ccgcccccgc
      421 cgcaggcggc gaggccaagg agcacggctg cagaggcggc gagcaaggcc cgcctgggaa
      481 tgtgcttcat aggtgtgact ccatcttctg cggggtgccg gggaaccggc gagggggtgc
      541 gtcggttgcg gactggggag ggtgcggagg tgcgggcacc cggtatgccg cgggcgcggc
      601 ataccggatc gggctagtag cggaagggcc agaaccggac gtagtccttc attcgtgtcc
      661 agatgcgatc gagccctcga ggctcgatga ggaggaacgt gatgatggcg atgccgaaga
      721 tggcgttctt gatggcgggc agttggtcgg tgatgaaggg gatcgagtcg ccggccttct
      781 ggcccacctc ggtgaggagc gcgggcagga gcatcatgaa gatcgcgccg tagaccgacc
      841 cggcgatgct gcccaggcct ccgacgatga tcatcgcgag gtagaggacc gagacgtcga
      901 gggtgaaccg ctcccaggtg actgtctcgg tgtaataggc ggacagtgcg ccggacaggc
      961 cgacgaaccc gctggacagg gcgaaggcgg tcaccttggc ccgggtgatg ttgacgccga
     1021 tggcctcggc ggcgatgtcc tggtcgcgga cggccatgaa cgagcgaccg agcgacgtgc
     1081 ggaagacgtt gcgggccatg aggacggcca cgaacgtgat cggcagcagg atccaatacc
     1141 actgcagttc aaaggtttcc cgctcgatga acggcaggcg gggcacctcg atgtagccct
     1201 tgtcctcggt gaggaagccc caccgccgga cgacgaacat gatgatctcc tggctggcca
     1261 gcgtggcgat cgccagatag agacccttga gccgcagcgc gggcagcccg aacagcgcgc
     1321 cgatggcggc cgtgacgagg atggccacga tgatcgccag cggcaccggc aggccgagcc
     1381 gggtggacag gatggccgag gtgtaggccc cgacggcgag gaagccgccc tggccgaggg
     1441 agatctggcc ggtgaagccc acgaggatgt tgaggccgac cgcaccgacg acggcgatga
     1501 ggatcgagtt ggcgatcccc aaccagtagg tgttgagggc gtaggggacg acgaggagca
     1561 gcacgatcac cagggcgagg cggaaccact cggccttggt atgccgcagg cgcagctccg
     1621 cgcgataggt gcggtggtgg attccggttg cggtagccat gggtcagacc ctctcgatcc
     1681 gggtctcgcc gaagatgccg tacgggcgca ccatgaggat ggcgacgagg acgacatagg
     1741 ggatgacctc cgctagaccg ggatcggcgt agccggagac gtactgcttg agcaggccga
     1801 tcgtcagacc gccgacgatg gtgccgggga tcgagtcgag gccgccgagg atgacgactg
     1861 ggaagacgat gaggccgaac gacgcgatgt tctgctcgac cgccgagagg tcggccagca
     1921 ggacaccggc caccagcgcg ctcacggcgg ccaaggccca cgcgagggcg aagatctgcc
     1981 ggaccgagat gcccatggtc agggccgcct gttggtcgtc ggcgacggct ctcatggcga
     2041 tgccatggcg ggaccgacgg aagaagatgg tgaaggccgc caggacgacc gcggcgatga
     2101 ggatgacgag cagtcggttg accggcatcg tcgcgccgag gaactcgatg ctgccggtgg
     2161 ggaggatctt cggctgctcg cgcaccgagg tgccgaagaa catctgcacc aaggccttga
     2221 gcaccgacga gagcccgatc gtcaccatga tgacgctgat ggcgctctcg ccgaccagcg
     2281 ggcgcaggac gaagcgttcg acgaggacac cgaggatgat cgccccgatg acccccacga
     2341 cgctggcgag gatccacggc agacgcatga cgacgaacgc cgtgtagaac atgtaggcgc
     2401 cgaagagaag gaactcgcct tgggcgaagt tgatgactcg ggttgccttg tagatgagga
     2461 cgaagcccag tgcggccagc gcgaggatcg cgccgtcggc caagccgtag acggtgagga
     2521 ggaggaagtt gctcatcggc ggccactcca cacggggcgt cgtccgcggc cctcgggcac
     2581 gcggtaggtg gtcaggtcga agatgcgcag gtcgatcgtt cggtcgacct tggtgccgtc
     2641 ctggtaggtg acggtcgtcg tgatgccgat gcgctcgtca ccgcggttga gggcgtcgat
     2701 gatgaggccg tagcggtcct ggatgacgtt gcgccggacc ttgcgggtcc gggtgatctc
     2761 gtcgtcatcc gggtcgaact gcttgtgcag caacacgaac cgctgcaccc ggatgctctc
     2821 gggcaggtcc tcgttggcgc ggtggatttc ctcggcgacc aggtcctgga cctcggcctt
     2881 ggcggccagg tcggtgtagg tcgtgtagga cagccgctcg tgctcggccc aagaaccggt
     2941 cgtcatcggg tcgatggtga tgatcgccgt catgccgttg tcggcggcga aggtgaccgc
     3001 ttcttcgacg tagggggaga acttcagctt gttctcgatg aaggccgagg agaaccgggt
     3061 gccgtcggcc gtcgtgagga cgtccttctg ccggtcgatg acgacaaggt ggccgttgtc
     3121 atcgagatag ccggcgtcgc cggtgtgcaa ccacccctcg gggtcgacgg cctcggccgt
     3181 ggccgccggg ttgcggtggt agcccttgaa caccgacgcc gagcgcagca ggatctcgcc
     3241 gttgtcggcg atctggatct cggtgcccgc gatgggggtg ccgacggtgt tgaaggcgat
     3301 gtcgtcgtcg cggtgaacga cggcgatgcc gcagatctcg gtctggccgt agatctgctt
     3361 gaggttgacg ccgatggcgt ggaagaaccg gaagacgtcg ggcccgagtg gagcgccgcc
     3421 ggtgtaggcg tgctggttgc gggccagccc gagttggtcg cggacggggc gcagcgcgac
     3481 ctggtcggcc agccagtgca gcgcgccgag cgcccccgca ccctggccct tgacccgccg
     3541 gccggcggcc ttgtcgccga cgtcgtagcc ccagccgaag accttgcgct tgagccagcc
     3601 ggcctcgtcg atgcggacct ggacctccga gagcatcccc tcccagatcc ggggcgggga
     3661 gaacatgacg tccgggccga tctcgcgcat gtccgacttc tgcgtcgccg agtcctccgg
     3721 gaaggacagg gtcaggccgc gggagagtcc gcaggcgacg gcgagcatct gctcgccgat
     3781 ccacgcgaac ggcaggaacg acacgtagcg gtcgttgctg tcgatcgggt cgatcgcggt
     3841 gaggtgatcg gccatggcca ggaggttgtc gtgcgacaac tcagccagct tgggacggct
     3901 cgtcgttccg gaggtcgtgc agatgacggc gatatccgag gacgtgccgc gggcgacctg
     3961 ctcgtcgagc cagccggggt gcgtggcgcc ccactcgcgg cccagtgcct caacggcggt
     4021 gaagtcgcgc aggcactcgt cggtgtattg ctccaggccg tgcgggtcgt agaagacgac
     4081 ggtgtcgacc agcagcggtg gcatgtcggc gaacggtgcc gaggtggctg cgctcgggtc
     4141 gaccgccgcg gcggcagcct ccgcgcgcag cttgagcaac ttgtcgacct gctcctggtc
     4201 ttcggcgacg acgaccttga cctgggccag ggtgaggatg tgcagcaact cctcaccgat
     4261 gctcgtcggg tagatgccga cgacggcggc gccgatgctc tgcgcggcca actcggcgat
     4321 gagccattcg gggcggttgt cgccgaggac cgcgacgatc tggccgcgtt caaccccgag
     4381 cgcggccagg ccgtgcgcga agtcctggac gcgcgcggca tacgtgctcc agctcatcgg
     4441 ctgccagatg ccgtagcgct tctcctgcaa ggcggtctgg tcggggttct tggtcgccaa
     4501 cccgcgcaac aacttcggga aggtcgcgtc actcgcgctc atcagtgctc ctccgccccg
     4561 agataggcct cgatgaccgc ggagttggct ttgacctcgt cgggtgtgcc gtcggcgatg
     4621 agccggccga agtcgagcac cgagacgcgg tcggagatgt ccatgacgac ggccatgtcg
     4681 tgctcgatga ggatgaccgt gacgccggcg agctcgtgga cgtcgaggac atagcgagcc
     4741 atgtcctcct tctcctcgga gttcatgccg gccatcggtt cgtcgagcag cagcagcgac
     4801 ggttgcagca ccagtgcgcg gcccagctcg acccgcttct ggatgccgta ggcgagcgac
     4861 cccaccggct tgtggcggaa gggctgcagc gagagcaggt cgatgacctc ctcgacgatt
     4921 ccgcggtgag aaagctcctg gttgcgagct ggcccaaacc acagcagaga ctgcagcacg
     4981 ccgtgtttca tgtgggtatg ccgccccaac atgaggttct ccagcaccgt gaggtgagag
     5041 aacagctcga tgttctggaa cgagcgggcc acgcccagcc gggcaatcct gtgcggcgga
     5101 agcttggtca gctcgtgggt gaccgcaccg tcaccggtgt gcaggaccgc cttgcccttc
     5161 tgcgggtggt agaggccact gacgcagttg agcaggctgg acttgccggc accgttgggg
     5221 ccgatcaccg catggatgtg accctgcgtg acggtgaacc ccacatcgtg cagggccacg
     5281 accccgccga accgaagttc gatgtcggag atctcgagga tcgggctccc cacggggacc
     5341 gtggaggagt tgagcgagtg cacataggct cggcccagca ccgcgacctc cttgtcgacg
     5401 acgacgggtg gttcaagcgg actctacttt tccactatct ggaaacgcaa tgcttcttgt
     5461 ggaaatcgtt acctcggcct acagtcgagc accgagccgg gcctgacgtg cgggacgctc
     5521 ggcacggaag gagtttggta ccggtgccgc ctctcagcga cgcactgcaa gaagcacctg
     5581 ggtcattggg aacagttcgc aacgccgcgc tcctgctgca gttgctggcc gaagggccgg
     5641 ccttccaaca gctcacggac ctcgccgatc gctccggact ctcgctggcg acggtccacc
     5701 gactgctgcg cagcctcgtc ctcgccgacc tggtcgagca ggatccgcag tcctcgcgct
     5761 atggcctagg ccccgagttg acccgcctgt cccaccgccg cctggcccgc ctgccggttc
     5821 tcggcgccct cgcgccctac ctcggtcagg tccgcgacgc cgtgggcggc accgtccggg
     5881 tcgatgtcgt cgtgcggacc gcagtggtgt cgatcgaccg gatcgacggc tcgggggccg
     5941 gggtcgggct ctaccgggac ccgcacggcc cgcgcctggc cctggacacg gtcgccggga
     6001 ggctgctgct ctcccgagcc gacgacgagc gatggaagca ggccctggcc gaggtcgacg
     6061 agcccactcg cgcgctcgcc gaacacgagc gcgacgactg ggcgaaggcg gcctggctgc
     6121 gcgccgacgg gccgttcccc ggcgcgcctc ccgaggtcgg cgtccccgtg atcaatgccg
     6181 ccggtatgcc gctcgcgacc ctcgtcgccg acctgcccct cactgcctcg ccggagcaga
     6241 tcgaggcggc cgtcgcccac ctgacccgtg ccgtcgcggc cgccgtgcgg acgctcggcc
     6301 atggctgacg gcataccgga ggtcggcctg cgagccagcg cggagccgga cgctgccgcg
     6361 tgggagcccg tcgtcgcggc cctgccgtgg tcgcggccac cggaccgact ctgggacgac
     6421 ggcccgcggc acggcacgtg gttccccggt ggccggatca acgcggcgac cgcctgcatc
     6481 agtgaccacg cgcgacgcac ccctgagcac gtcgcgctgc tctgggaagg cgagcccgga
     6541 gaccgtcgcg agctcacgta tgccgccctc gacgccgacg tgaccgccct ggcccgggcg
     6601 ttgcgcgggc tcgggctcgg tgtgggcgac gtcgtcgccc tgcacctcgg ctggatgccc
     6661 gagacggtcg tggcgatgtt cgcctgcgcc cggatcgggg ccgtcttcgc cgtcgtgccg
     6721 acgccgctac cccctgaggc gttggccgac cgactgcggg ccatcggcgc ccgactgctc
     6781 ttcacccagg acggcgcctg gcggcgcgga accgtgctgc ccctgaaggc gcgggccgac
     6841 gaagcgctgt cggcgctcgg cgggatcgag cacaccatcg tcgtgcgacg cacggggatc
     6901 gacgtcgggt ggtacgaagg cgaccggtgg taccacgacc tgctcgtcgg ggtccgtccc
     6961 ggcctgcgcc gcgaaccgtc cgaggccgac accccggccg acctgccggc cgaccacccg
     7021 ctgcaggtgg tcagcctggc caaccgccgc gggcagccgt tgacggtcgc gctcggcacg
     7081 gggcacacgc tggcgatcgc ccgtgccctg caccacgcgg gcatcgccga cggctccatc
     7141 ctctggtgcg ccggggagaa ctcctggctc ggcacccagg ttcacggggt ctacggcccc
     7201 ctcgtcgccg gccataccgc ggtcctctac gagggaacgg tcgacgtccc gacccacgcc
     7261 cgcgcctggg aactgatgcg ccgacatggc gtgaccacct tggtcacgac accgtcggtg
     7321 ctgcgggcga tgcggtcgtg gtcgttcgag ttggccatcg gcgatgagcc gatcggcgcg
     7381 gtccctcagt tgcgccgggt cgtcgccttc ggggagccca tggatgccga ggtcagagag
     7441 tgggcggcga ccggcctggc cgggcatccg gtgtcggtgg ccgacgggtg gggccaggtc
     7501 gagctcggcg gaatcgtgtt gctggacaac ccgatcgacg cgtctgcgat gcccgacctc
     7561 ggccgggtgc tcgtcgaccg cagtggcgag gtggctcccg acgggatgcc cggcgagctc
     7621 gtcctcaccc gtccctgggc cggcacactc gtcggggtgt ccgggccgcg ggagaccctc
     7681 gacgacgccc actggtctcg gctgcccggg tcgtatacga caggcgacgt ggcgcggtcc
     7741 gagcccgatg gcggcctgga gttcctcggc cgcaccgacg aggtcacctc ggtgtcgggg
     7801 caactcgtct cgctcagcga ggtgcgggat gtgttgcttg agcacccgtt cgtttccgcc
     7861 gccgacgtcg tggagcgccg cgatcaactc cgcgggcggg tcatcgctgc ggccgtcgtc
     7921 ctcaccccgg acggtgtggc ctccgacccg accctcgtcg cccgggatct cggccgcagc
     7981 gtccgggaat cgttgggcgg gttggcccgg ccgcgggtgc tcatgcttgt cgaccggttc
     8041 ggcgacgagt tgcgaggggt cgagcggcga cgggctctgg ccggcatacc cgtgccggaa
     8101 ggccccgaac cgcggcgggt cacctgggag cagttgttgg tcgcggccga ccacctcacc
     8161 tgagccgcgg ggcgcagagc gcagagcgca gagcgcagag nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn ccgtcagatg ttgccgtcga aggccgccgt cgggtcgtcg
     8341 gcgacgaggt gatcctcgac ctggttgatg atgacgatga gggcccaggc cgcgatgagc
     8401 gtggccagtt gctggacgcc ggtgccgatg gtgccccaga tgagccacgt cagctcgaac
     8461 tgcggttgcg ccaggaccgt catgacggcc gtcgatcccg agccggccag gagccaggcc
     8521 ggccaccaca cgaagaacac cgaggggacg ggcaaccgcg cgcccacacc ccccagccgg
     8581 gcggcgtctg ggcgggacgc gtgccagagg tccttgacgg cctgggccgg ccaccacaac
     8641 tggaggacgg ggatgatcca ggagaagatc gccacgcctg tcgaacgccg cagcacccgg
     8701 tgatcgaccc gggtgtcggt gtatcgaatg cccaaccaca cgaggaacag aatcccggtg
     8761 atggtgcgca gccccagatc tgcccaggtg gccaaggtcg acaggaggag gagtttgtcg
     8821 acctcctgct cggcggccgg cgtgggctgc agccgccaca ccgagagggc gttgaagacg
     8881 atgatcgtgt agaggagcag ggctgcggcc aagaggccgc aaagcgtcaa cagcccctga
     8941 accaccccgc cgagcgcccg cgagccgctt cgtccgccct cggccaacgc ctgctgggtc
     9001 cgcgcatagg tgccggggcg caccgacggc cgagtttcgt cggtccagtg cagcccgtcc
     9061 cagaaccgct cggtgtcggc gaggttgggg tcggcgcgcc agccctccgg ggcaccgatc
     9121 gggaacgcgg cggccgggtc gtaaggaacc gtcgacatgc gcccatcttt gacgcggcgg
     9181 gcagggaggt cgagtcctcg cccgaaatcc acccgttcgg acgacccact agcatccccg
     9241 gtatgccgcc cgcacccgct ccgctcggga ctctcgactg gcgtccgctc gccgaccacc
     9301 tcgacctggt cgccggtccg gttgcggctg ccgcggggct cgtgcccgat gctctggtgg
     9361 cgcccatcga cgcgacgctg gccgacaccg cggcgttctg cgcggcatac caggtgccgg
     9421 cggaggcctc cgcgaactgt gtggtggtcg agggacgtcg ggccgagcgg gtggtgcgcg
     9481 cggccgtcat ggtgctcgcc gtcgaccggg ccgacgtcaa caaggtggtc cgcaagcacc
     9541 tcgacgtgcg caagctctcg ttcatggggt ccgaggcggc gcaggaggcc accgggatga
     9601 tccagggcgg gatcacgccg gtggggctgc cctccgactg gctgatcctc gtcgactcgg
     9661 cggtggtcgc ggcagggccg gtggtcatcg gcggcggcgt gcgcggcgcg aaactgttgc
     9721 tgcacggcgg cgatctcgcg gcgctgccgg gggcggccgt gctggacctc gccgtcccgc
     9781 gctgagccgg ggaaagcggt tgtcccgcgc cactaccctt gggggatgcc ctccccgcac
     9841 gacacgctcg cccctttggt ctcggccgcg atcgcgaccg cgttcggcga cgagttgcgc
     9901 ggtgccgacc cggtgctgcg cctcagccag ttcgccgacc tgcaggccaa tttcgcgttg
     9961 ccgctggcca agcgcctcgg tatgccgccg cgcgaggtcg cgacgcgggt gctggccgcg
    10021 ctcgacctga ccggagtgtg cagcggtatc gaggtgagcg gcccggggtt cgtcaacctc
    10081 accctcgacg actcatggat cgcgtcgtcg gtgcaggccg tcggggccga cccgatgctc
    10141 ggcgtcacgg gcgatgcgcc gcgggtcgtc gtcatcgact actcggcgcc caacgtcgcc
    10201 aaggagatgc acgtcgggca cctgcgcacg accgtggtgg gggacgcgct cgcccgcacg
    10261 ctcgagcatc tcgggcaccg ggtgatccgg cagaaccacg tcggtgactg gggcacgccg
    10321 ttcggcatgc tcgtcgagca cctggtcgag gtcggtgagg gctcgtccga ggcgctggtc
    10381 gtcgagaccg accccaacgc cttctatctc gcggcccgga cgaagttcga tgccgagggg
    10441 tcgtttgccg accgggcccg ggcgcgcgtc gtggcgctgc aggccggcga ccccgagacc
    10501 ctgcggctct ggggtgagct cgtcgggttg tccaagagct atttcaaccg gatctacgcg
    10561 gcgctcgagg tgacgctcac cgacaccgat ctggccggtg agtcgaccta caacgacgcg
    10621 ctcgcgggga tctgcgagga gctcgaggcc aaggggatcg cgacgatctc cgacggcgcg
    10681 ttgtgcgtgt tcctccccga gttcaccggc cgcgaaggca agccggtgcc gctcatcgtc
    10741 cgcaagtcgg acggtggata tggttacggc acaacggatc tcgcgacgat caagcatcgt
    10801 gtggaggatc tcggggccga ccgggtcctc tatgtcgtcg gcgccccgca atcgctgcac
    10861 ttccagatgg tctttgcgac cgcccggttg gccgggtggt tgcccgacac cgtcgaggtc
    10921 gtccacgtcc agatcggcaa cgtcctcggc gacgaccgca agatcctgcg cacccgcgag
    10981 ggcaaggccc tgcgcctcgg ggcactcatc gaggagtcgg tcgacgccgc ccaggcggtc
    11041 ctcgacgagg cgcggcccga cctcgacccg gcgacacggc ataccctcgc gcgggagatc
    11101 ggcgtcgggg cgatcaagta cgccgacctc tcggtcgccc acgactcgga gtacgtcttc
    11161 gatctcgccc ggatggtctc gctcaccggc aacaccggcc cctatctgca gtatgccgcc
    11221 gcccgggtcc gctcgatctt ccgctccgcg ggggtcgaga tcgaggtgcc ggtcggggga
    11281 gcgcagcggc ggatcgtcgt cactgagccg gccgagcggg cgttggcgct ggcgctgctg
    11341 gagttcggct cggtggtgcg gggcgtcggg gccgagttgg agccgcaccg gttgtgtggc
    11401 tacctgttcg acctggccca gacgttctcg gcgttctacg aggcgtgccc ggtgctcaag
    11461 gcccccgacg ccgagacgcg cgagtcgcgg ctcgggctgt gcgcgacgac cttccgggtg
    11521 atggagcagg gtttggcgct cctcggaatg gcgacgcccg cccagatgtg acgtcggggg
    11581 gcgagacgcg gccgtgtgcg agggcttgac gcggctgcgt gcgaggccgc gccgcttcag
    11641 ccgcgtgcgc cacggagtgc gatcatcggg gtgatcaccc caccaagcgc actccgtcag
    11701 gcactcactc gcggccgtca acgccgggcc ccatggttat gcaggtgccg aagcttcgag
    11761 cgtcacctcg accggttcgg gtgcccgagt gccagacacc gtgagcagga gaacgcaatc
    11821 ggtgagggcg tccacgccgt gccgctcggg tgggatcgcg gcataccctc cggcgtccag
    11881 gacccactcg cgaccagtgg cccagagccg agcctgcccg gacaggcact gcagggtggc
    11941 ggcgggcggg gcgttgtgct cggcgagcgc cgcgccctcc ttgagcgcga tgaccaccgc
    12001 gcgcagttgc ggggcggcga acagcgtcga ggccgcccga ccgctctcgg cgtcgcgggc
    12061 cgtggccagg agggagtcgc cgagtgcggc gaggtgtgtc acgggttcca tccccacacg
    12121 gtatgccgcc caagcagcgc gcggtcgtca agcctcgagc cagttgggcc cgccggagag
    12181 ttgccgtaaa tcgggcacat aggatatagt tgaaatcaaa actatcctcg gtccacgcac
    12241 accctcaaag gagggccgca tgccagccat caccgtcccg gacctcagcg tcctgccgtc
    12301 gatcccggcc cccggcccgg ccgccacgca gcgcccggtc gtcagccgca cgaccgcgcc
    12361 gcacgggctc gagggtgagg gcttcccggt caagcgggcg ttcgcggggg tcgacctgag
    12421 cctgctcgac ccgttcatca tgatggacga gatgggcgag gtcgactacg ggccggggga
    12481 gcccaagggc acaccgtggc acccgcatcg gggcttcgag acggtgacct acatcatcga
    12541 cggcacgatg gatcacgccg actcctacgg cggcggcggg acgatcacca acggcgcaac
    12601 gcagtggatg acggccgggt cggggatcct gcacatcgag gcgccgccgg agcaactcgt
    12661 taccagcgga ggtctgttcc acggccttca gttgtgggtc aacctcccgc gcaccgacaa
    12721 actggccacc ccgcgctacc aagaccttgc ggcgaccgat gtcgccctgc tgtgctcgcc
    12781 cgacggaggt gcgctcgtgc gggtcatcgc gggtgacctc gctggccacc gcgggccggg
    12841 ggacacccac acgccgatca ccatggtcca cgcgacgctc gcgccgggcg cacgcctcga
    12901 cctgccgtgg cgtcgggact tcaacgccct cgtctatgtc atgggcggcg ccggcaccgt
    12961 gggcccggcc gacgcgccac tgcgggccgg gcaactggcc gtccttggca cgggggacgc
    13021 cctcaccgtc agcgccgacc ccacccagga gagccggtat gccgcgggcc tggacgtcgt
    13081 cgtcctcggc ggtgccccga tccgtgaacc gatcgcctgg gccgggccgt tcgtcatgaa
    13141 cactcgcgcc gaggtcgtct ccgccttcga ggactaccag gccgggcggc tcggccaccc
    13201 gcttccgcac gcctgatcgc cgccctggcc gcccggcggg gtggacggca gggtggtcgg
    13261 gacgcgaagg tgagcgggcg gcatacccca tgggttgtga gacctctccc actgacctag
    13321 gtccctttgt tccctccggg ccaaccccat aggtttgtga catcgccggg tcgacccggc
    13381 atgccgtagt gcgctcgcgc tgcggggaca cacgccgacg cagggagaac acgtgctcat
    13441 cggtgtccct cgggagtcgc ggccgggtga gacccgggtc gctgccaccc ccaagaccgt
    13501 cgaacaactt ctcaaactcg ggtatcaggt cgctgtcgaa gcgggcgcgg gtgccgcggc
    13561 aagtttcgac gatgccgcgt atgccgcagc cggcgccacg gtcgcccatc cggccaccgt
    13621 ctggggcgcc gacatcgtca ccaagatcaa cccaccctcc gacgaggaga tcgcgctgct
    13681 tcgccagggc gcgatcctcg tgagcctggc ccagccggcc ctcaacccgg cactggtcga
    13741 caaactcgcg gcggcggggg tcacggcgat ggccatggac gccgtgccgc gtatctcgcg
    13801 cgcccaggcc ctcgacgtgc tctcgtcgat ggccaacatc gccggctacc gcgcggtgat
    13861 cgaggcggcg cacgagttcg ggtcgttctt caccgggcag gtgacggcgg cgggcaaggt
    13921 gcccccggcc aaggtcttcg tctcaggcgc cggcgtggcc gggctcgcgg cgatcggcac
    13981 ggccaacagc ctcggcgcga tcgtgcgggc caccgacgtg cggcccgagg tcggcgagca
    14041 ggtcgagtcg atgggcgccg agttcgtcaa gcccgacgtg cccgagcaag aggtcagcag
    14101 cgacggctac gccaaggagg tcggcgcgga ctacgccgcg gccgccgccg tgatgtatgc
    14161 cgcgcaggcc aaggactgcg acatcatcgt caccaccgcg ctcatccccg gtcgaccggc
    14221 ccctcggctg atcaccgaag agatggtcgc cgcgatgaag cctgggtcgg tcatcgtcga
    14281 catggcggcg tccaacggag gcaacgtcgc cggctcggtg gccgacaaga tggtcgtcac
    14341 cgagaacggc gtgaagatca tcggttacac cgacctgccg ggtcgactgc cgacgcaggc
    14401 cagccagctc tacggcacca acgtcgtcaa cctcatgaag ctcatcaccc ccggcaagga
    14461 cggggtcgcc gtcctcgact gggaggacgt catcgtccgc gggatgacgg tggccaagga
    14521 gggcgcggtc acctggccgc c
//
LOCUS       DAORPW010000051        13849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_890_length_13849_cov_481.048137, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000051 DAORPW010000000
VERSION     DAORPW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13849
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_890_length_13849_cov_481.048137"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            709..1842
                     /gene="moeB"
                     /locus_tag="PLB86_06130"
     CDS             709..1842
                     /gene="moeB"
                     /locus_tag="PLB86_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HRC64748.1"
                     /translation="MSAQELTRYSRHIILPGVGVPGQQRLREARVAVIGAGGLGSPVI
                     LYLAAAGVGTIGVVDDDVVDVSNLQRQVLHSSSRTGMLKTESAKIAVHELNPHVEVIA
                     HSVRLSPENALSILAEYDIVVDGTDNYEARYLVSDACVLLGKPLVWGSVFQFEGQVSV
                     WWAGRGPCYRCAFPEPPAPGLVPSCAEGGVFGAMCASVGAAQVAEALKLVLGIGQPAL
                     GRLLVYDALGSRWRTLTLLADPQCPACGHAPTITDLDATRTVCGVASRDTLIAEVTPE
                     DLAAILADRPESVRLIDVRGPQERAIVSVPGDENIPLQQFEDGSALRLLSARDDLILY
                     CRSGIRSESAARLIPDGHVNSVKHLAGGILEWIRQVSPGLPTY"
     gene            complement(1836..3074)
                     /locus_tag="PLB86_06135"
     CDS             complement(1836..3074)
                     /locus_tag="PLB86_06135"
                     /inference="COORDINATES: protein motif:HMM:NF019429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA domain-containing protein"
                     /protein_id="HRC64749.1"
                     /translation="MAHGEARVIGLVDGVFGSVGSVWHKEILFAISRGCQVFGGASMG
                     ALRAAECRSFGIRGVGTVYAAYADGELEDDDEVAVSHLDAAFGYECLTVPMVTVRAAL
                     SAAVSVGLLPERDHERALQAAKNVFYADRTWDVLGREWGALDQDWGPDVTLFAQQPQH
                     DVKAVDAAAVLRAVQSARPEDGPRQADWQMEPTVFWRRLVQETDFPSVAPWFEDDGAQ
                     VSLARRIDVVRGALLDPGHAQLGTRARLFAHLVYQVCEALGIAPSAREVQERSERIRR
                     VLGLGDARTTRQWLLASKLTVAEWQRAIRFQLMAERLGEEYAAELRHQVPMLVAASGG
                     WAQVEHLAEPMQLGVEGESPSVVLTALATRPDMPSAATLEDLAARLGFTSLTEFLSTT
                     GVDPGDTQAATDVVERRAPQ"
     gene            complement(3182..4420)
                     /locus_tag="PLB86_06140"
     CDS             complement(3182..4420)
                     /locus_tag="PLB86_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HRC64750.1"
                     /translation="MTSVSTQDPVSEDLKRYRRGTHRACDPSETYDHLRALMPRCGIT
                     RIANITGLDRVGVPVYTAIRPNSRALSTSQGKGMDRDAARASALMEAFESWHGERVSA
                     DGGYYRREEAEDENGIPVADVGRISRRGRDVSWLSSTRINWVRGLDVMSGVAVLLPYD
                     CVSSDFTGDVQRVAFIRSTNGLASGNTLTEATLHGLYEVIERDAVALWSPSAGTADRV
                     VDPGTVHDEDNAALLDRLADTGLGVLIRDVRCDTRVPVFAVAIAPTNPTRMPPFAAFS
                     GYGAHLDPRVALSRALTEAIQSRLTVIHGGRDDLWPSAYARVLDHRAARNWARFVSTA
                     EPTVDFERIPDHSTETFSGDLEVLKESIASVAAEAIAVDLTRPGLGIPVVKVVVPGLS
                     GIPFDPRPRDGGRVGSAAPA"
     gene            complement(4417..5100)
                     /locus_tag="PLB86_06145"
     CDS             complement(4417..5100)
                     /locus_tag="PLB86_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HRC64751.1"
                     /translation="MHVDGRSLSVLILKDCRMQWKAFLVLVTFEILTLLSFVAQFPEP
                     MRASAGAFLQGLTSIGTFVMAYRLLATEESSGTIRFLKSLPLTNDEIFWSKFLFMSGY
                     AVLNAAVVNLVYAGLRPLISWSWQLPTMPGVLGGVVVQLVFAVILLSIAILANSEKAI
                     WVPFPLVILLLNGYTLMTSPNGWDPGARVLASIQQNWALWSIAVVGVLLAVARGVVHA
                     AGVKRSLVK"
     gene            complement(5090..5812)
                     /locus_tag="PLB86_06150"
     CDS             complement(5090..5812)
                     /locus_tag="PLB86_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC64752.1"
                     /translation="MLSGRETLSLDGVGVRLGGFVLSGVTFAVGPGEVVGLVGHNGSG
                     KSTTFRIIADLIRPTSGSITLGDLDHRADERAFKRRVCFVGDNNQTYAGMTVGAVLAF
                     ARRLYPNWDHDWCAQLCDELRLPLDTKVAHLSAGMRTKLGLVLGFSPRPDVIVLDEPT
                     AGLDGASNEWIWQVVDRQSVEAGLAVIVSSHSRDDVAQHCSRVVLLEQGQIIDQIDIA
                     GDRALARERLEGAMGRGGGHAR"
     gene            complement(5796..7004)
                     /locus_tag="PLB86_06155"
     CDS             complement(5796..7004)
                     /locus_tag="PLB86_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64753.1"
                     /translation="MHRTLRHDLILGEETAEGRVTVTDPVANRHYLMTGATVRVLRMF
                     DGSRDDNQVYAALAGIGVHVSDQTFSAMAQRADQLGLFQGGQPPPVPSHLRGPQRGNL
                     LFLLLGEFDPHRVVDALAPVGRVLFSRMAAGTLLALLAWCVWLVVQHSDRYVADMTAF
                     AMVSAWALVYLANAVVTVMHEFGHALATRRFGGQVHRMGLALYLLSPAAYTDTSAAWA
                     FSKNRHRIVVNLAGIYVESFVLAGCVLLWVFDVLPVTFAAANFVLLHALLARIIFNLN
                     PFLRLDGYWLLSDLMHIPNLRPRALRTLLALLSGHRRSAPAVASTEERAFLVVYGILS
                     VAFAVGGLVVGALVIDGLARRFVPWYSNSLALVLGVVLGLLALLNAWRYTKALRRQVF
                     LEGGAHAQRS"
     gene            complement(7037..8353)
                     /locus_tag="PLB86_06160"
     CDS             complement(7037..8353)
                     /locus_tag="PLB86_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HRC64754.1"
                     /translation="MTTDLAFERTLHLANQLISPVTGVLKRSLLLPLVGGQAPYAHFA
                     AAPASYGTLPTGGDIANPGASAWDLRQCLSQVTFEAVERYCAAFVDYSALLLSKPEGP
                     AFDVGCAIQRFAEFQYGRSDFSYVPLRQDSVIHWAVGRSLFTGQRRYIPASFTYLPYR
                     PTSPDEVIGPSFSTGMSAAWTLDDACLSGLLEVVERDAFAITWMGQLVRPRLEPRPDS
                     ELARRVAAVEADGCSSVTFVDLTTDLGLPVVCAVVRRPALGRVLTTVGLSSKLCPSRA
                     CDKALCEALSDHERLRTELADPRRPPWTPAPDFSDVVDFEWHGRAYTDPRAQLALDFL
                     TSSSEVHQIDGEFAPPQAGDLVRTLRQLEEHCSDVVMVDLTTRDMHELGLVVVKIFVP
                     ELVPLNADHRYPYLGHRRLHTHGGQRPDGTQEQLLTHLNSYPHPFS"
     gene            complement(8350..9213)
                     /locus_tag="PLB86_06165"
     CDS             complement(8350..9213)
                     /locus_tag="PLB86_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRC64755.1"
                     /translation="MPEPLNKQSYSLVDFFEEDDYSESETYNEYTKLTRMNVARISHR
                     VEQIKSDPAMLAMMARSWKSYPGSTVTPLPPFRAPTVGFAETVLRRRSVSSLGRDFVG
                     EAITAEQLGGTLVMAYGPTSKIESRTGDVQHLRATTSAGALFPTEIYVLAFNVTGIEP
                     GLYHYRPIEHSLELIRSGDLRAQFAGLSSYQGLCLTSSVALVLTSVLKRTMSKYQHRG
                     YRFAMYDCGALTQSLYLSGTAMGLDTCALGGIYDDEVAAFLGVNPVDEPVQLGFLLGP
                     RGAEMGTEKQQ"
     gene            complement(9253..10560)
                     /locus_tag="PLB86_06170"
     CDS             complement(9253..10560)
                     /locus_tag="PLB86_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HRC64756.1"
                     /translation="MGVESRQDLGGALDWMVGPTSIVRAVRTILNAPGEPRLFSVASV
                     PNSLTSIAGAEMAGHTGAMAETWEQAAASAIGECVERYCCAVQPPNLLTAPARQLGEG
                     ALLVDDFELFDERQYAHPQFPFARHTEDLPMTWVPAARIRDGEPRFVPACLVYIPYVP
                     RLVEHSDMVALSVSSGQACHSDADLALLSGLYEVIERDAFMLTWVRRLPPTRLNYRDD
                     PVVGPWFDRYFADSSLTFDVFRLRSDIAVPSVLCVARGIHHQGPFACVGASCRLDESE
                     AVRKAIVEAAQGAVWVRDLIDTKPHWEHGPDYANVRDFEDHVRLYGDPGMLEHLDFLY
                     VDEWDGLAPAPDVTGLRAQREQVLDCLEAVGLQALVVDITTRDVADAGLVVLRVLIPG
                     SVQLYSVHGLPNFGARRYHTVPPRLGFDADIHRSYNPVPHPFP"
     gene            complement(10565..11713)
                     /locus_tag="PLB86_06175"
     CDS             complement(10565..11713)
                     /locus_tag="PLB86_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HRC64757.1"
                     /translation="MTASAPQRGNLRLGEVYLVPAADGGLTMRTPRRSAVLRGRLVSE
                     VFPRLLPLLDGTRDFEGVLTELASDGEFPNLEASLRQLLDMGVVEFAEPAAELEGVPS
                     QLLPKLRGQDRFMRQHGGDDGTTARIASGSVLLAGEGPLMPDVAVGLAASGVRRIVVA
                     ARRTVGAVDVGQSTHIGPTDLDRSMGEVVAAVLKRNDLPTLVEPAPFPESKLGWQQLL
                     GDIDVAVVVQDAPVVSLPWMDEFNRATIAAGTPWTTGALLQRANVHVGPSVIPGQTAC
                     WKCFEFRFKSNLSSVVRYEEFQDYVASLERYVEHGSLPSVTAFTGAMVALEGVRLLAA
                     GDVFVRTAGSLLAFDLWELRLETHPVLKLPRCPHCSTVSSVPQERTWS"
     gene            complement(11992..12135)
                     /locus_tag="PLB86_06180"
     CDS             complement(11992..12135)
                     /locus_tag="PLB86_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64758.1"
                     /translation="MNRSTEVELVDEMFSIEELEQRETPGSWSCGCSCSCSCTSCTCV
                     YTW"
     gene            complement(12364..12963)
                     /locus_tag="PLB86_06185"
     CDS             complement(12364..12963)
                     /locus_tag="PLB86_06185"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRC64759.1"
                     /translation="MTEAERTMRRRRIVVRIVLFVTIIGLGLAVVSGTLGMKPGLLTH
                     TDDPIMVGGSGPGGEVNSVIPSISGVNYANQPVSVPTGRPVVLLVVAHWCEYCKADLA
                     LMSQGHAAGTFRPKADIVIVSTRHLPFLSWPPSEALSLDSVPFPVIVDTDSSIATYLA
                     LRVTPTWYFIDRTGTIRKVVAGTLSLAEIDDEINAVTRE"
     gene            complement(13497..>13849)
                     /locus_tag="PLB86_06190"
     CDS             complement(13497..>13849)
                     /locus_tag="PLB86_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRC64760.1"
                     /translation="LLRRVMRRPLLGYAVDGSGDVYVRGQVPTVALDGRYLDLLVGAL
                     LEAADEPFNDLLAIGFLTSMKREWAWRVARGESLANLEAFRSILAGSEDDPLYAVGPL
                     PVDPATPDDRADSR"
BASE COUNT         2607 a         4510 c         4506 g         2226 t
ORIGIN      
        1 ttggcgttcc actcggagtg gccgtgacgc agcaggatga gggtgtatgc catgcggccc
       61 agcctattgc gaccgatcgc ctcagggtct agcggtccag cgtccgctac gtcacgctcc
      121 tccgcgcgct gacctgaggc gggcgaccat ggccggcctg ggactatctc agattcggtg
      181 tttctggtcc gtggcggacc agaaacacga atttgagata gtcgcccatg gcgaacgcat
      241 ggggagggtc ggccccgccg ctttacttcg gctgtgggca agagttccga ccggggcccg
      301 gtgggagggg aagcggcact tctccccagg cgtattcaga gcgccgggga tacacttggg
      361 gacagtgaga tttcgttcgt cgatgcggac ggaaaaggta cccctggaag cgctcgggcg
      421 cccctctcaa agggcacccg ctggaaagga cgctctcgtg tcgatccatc gctcgtcgtt
      481 cttcgttcca gccgttgttt tcaccctgtc cgtgtttgcc gtccgcctcg gggaccccgt
      541 ggtttggatg tggcgcgacc agccgatcgt cggggtcctt ctcctcggcg ctgcggtcgt
      601 cttctggctg ctggtcctgc ggcaggtccg gcatgctcgg cctccgtttg agcctccgtc
      661 gaccgggcag cagcaaggcc ggggccggag atgactgaca caaggtcctt gagcgcccag
      721 gagttgactc gctactccag gcacatcatc ctcccgggag tcggcgtgcc gggccagcag
      781 agactgagag aggcgcgggt cgccgtcatc ggcgcaggcg gactcggatc accggtgatc
      841 ctctacctcg cagccgcggg tgtcggcact atcggggtgg ttgacgacga tgtggtcgac
      901 gtgtccaacc tgcagcggca ggttctgcat agttcgtccc gcactgggat gctcaagact
      961 gaatccgcca aaatcgctgt gcacgagctg aatccgcatg ttgaagtgat cgcccactcg
     1021 gtccggctgt cgcccgagaa cgcgttgtcg atcctcgctg agtacgacat tgtggttgac
     1081 ggcacggaca actacgaagc gcgctatctc gtctcagacg cttgtgtgct cctcggcaag
     1141 ccactcgtgt gggggtcggt cttccagttt gagggccagg tatccgtgtg gtgggcaggg
     1201 cgcggcccat gctaccgatg cgcctttcct gagcccccag ccccgggtct tgtcccgtcg
     1261 tgcgcggagg gcggggtgtt cggagccatg tgcgccagcg tgggagcagc ccaggtcgcc
     1321 gaagcactca agctcgtact cgggatcggc cagccggcgc tcgggcgcct gcttgtctac
     1381 gacgcgctcg gatcacgctg gcgaacgctg acgctcctgg ccgatccgca gtgcccagcc
     1441 tgtggacacg cccccaccat caccgacctc gacgcaacga ggactgtctg cggggtggcg
     1501 tcgagggaca ctctcatcgc tgaggtcacg cctgaggact tggccgcaat ccttgctgat
     1561 cgaccggaat ctgtccggct cattgacgtg agagggcctc aagaacgtgc cattgtctcc
     1621 gtacccggag acgagaatat cccactacag cagttcgagg acggatcggc acttcgtctg
     1681 ctatcagcgc gcgacgacct tattctctac tgtaggtctg gaatacgctc cgaaagcgcg
     1741 gcccgcctta tcccagatgg ccacgtcaac agcgtcaagc atctcgccgg cggcattctg
     1801 gaatggatac ggcaggtgtc tccgggcctg ccaacctatt gaggcgccct cctctcgacg
     1861 acgtccgtgg cagcctgagt gtcccccggg tcaacgccag tggtcgacag gaactcggtc
     1921 aacgaggtga acccgaggcg agcggccaga tcctcaagag tggccgctga cggcatgtct
     1981 ggtcgagtcg ccagcgccgt cagcaccact gatggcgatt cgccctcgac gcccaactgc
     2041 atcggctcgg ccaggtgctc gacctgggcc cacccgcccg aggccgcgac cagcatcgga
     2101 acctggtgcc gcagttccgc ggcatactcc tcgcccagcc gctctgccat caactggaac
     2161 cggatggccc gctgccattc cgctacggtc aacttggagg cgagaagcca ctgccgagtg
     2221 gttcgggcat cacccaggcc gagtactcgc cggatccttt ctgagcgctc ctgcacctca
     2281 cgcgcggatg gggcgatgcc tagagcctcg cagacctgat agaccaagtg ggcgaacaac
     2341 ctggcccgcg tgccgagctg cgcatggccg ggatcaagaa gagcgccccg gaccacgtcg
     2401 atacggcggg cgagtgaaac ctgcgcgccg tcatcctcga accacggagc cacggacggg
     2461 aagtcggtct cctgcaccag ccgacgccag aagacagtcg gttccatctg ccagtccgcc
     2521 tggcggggac cgtcctccgg gcgagccgac tggacggccc tcagcaccgc ggccgcgtcc
     2581 acagccttga cgtcgtgttg cggctgctga gcaaacaggg tgacgtccgg gccccagtcc
     2641 tgatcaagag caccccactc ccgcccaagc acatcccagg tccgatccgc gtagaagacg
     2701 ttcttcgcgg cctgcagtgc ccgctcatgg tcgcgctcgg gcaggaggcc gacgctcacc
     2761 gcggcactca gcgcggcacg cacggtcacc ataggcaccg tgaggcattc gtagccgaat
     2821 gccgcatcca gatgcgagac cgcgacctcg tcatcgtctt ccaactcccc gtcggcatat
     2881 gcggcataca ccgtgccgac tccgcggatg ccgaaacttc gacactccgc cgcacgcaac
     2941 gctcccatgc tcgcgccgcc gaagacctga cacccacggg agatggcgaa gaggatctcc
     3001 ttgtgccaga ccgatcccac tgatccgaag accccgtcga ccaaaccgat gacgcgggcc
     3061 tctccatgcg ccaccgcacg atagacatcc cctgcgcgtg cgggcgggag caaggtgact
     3121 tccggcgcga gcgcttgcgc ggcgttcggc gggatgctcg ggccaacaaa ggcaatcctc
     3181 ctcacgcggg tgccgccgac ccgactcggc ctccgtcgcg gggacggggg tcaaagggga
     3241 tgcccgagag gccaggaacg acgaccttca ccacagggat gccgaggccg ggacgggtca
     3301 ggtcaacggc gattgcctcg gcggccaccg aggcaatcga ctccttgagg acttcgagat
     3361 ctccggagaa ggtctccgtg gagtggtcgg ggatccgctc gaagtccacc gtcggctcgg
     3421 ccgtggatac gaaccgagcc cagttccgag cggcccggtg gtcaaggact ctggcatacg
     3481 cggaaggcca aagatcgtct cgacccccgt gaatcacggt gagccgactc tggatggcct
     3541 cggtgagtgc ccgagagagc gccactcgtg ggtccagatg ggcgccgtac ccggagaagg
     3601 cggcaaaggg cggcatccga gtcggattgg tgggcgcgat cgcgactgcg aacaccggca
     3661 ctctcgtgtc gcacctcacg tcgcggatca gcacgccgag cccggtgtcc gcgagtcgat
     3721 cgagaagtgc ggcgttgtct tcgtcgtgaa ccgttcccgg gtcgacaacc cgatcggcag
     3781 tgccggcgct cgggctccaa agggcgactg cgtcgcgctc aatcacctcg tacaacccat
     3841 ggagggttgc ctccgtcagg gtgttaccgc tggctagacc gttcgtcgaa cggatgaacg
     3901 caacccgttg cacgtctccg gtgaagtcgc tggacacaca gtcgtacggg agcagaaccg
     3961 caacgcccga catgacgtcc aggcccctca cccagttgat ccgcgtcgat gacagccacg
     4021 agacgtctcg acctcggcgg ctgatccggc ccacgtcggc caccgggata ccgttctcgt
     4081 cctccgcttc ttcccgtcgg tagtagcccc cgtcggcact gacccgctcg ccatgccagg
     4141 actcgaaggc ttccatcagc gccgacgctc gcgctgcgtc ccgatccatc ccctttccct
     4201 gggacgtcga gagcgcacgg gagttcggtc ggatcgcggt atacaccggg acgccaaccc
     4261 ggtcgagtcc ggtgatgttc gcaatgcggg tgataccgca cctgggcatg agcgcccgca
     4321 agtgatcgta ggtctcggac gggtcgcagg cgcggtgcgt cccccgccga taccgcttga
     4381 ggtcctcgct caccggatcc tgggtagaga cggacgtcat ttcaccagac tccgcttgac
     4441 cccggccgcg tgcaccaccc cgcgggccac cgcgagaagc acaccaacga ccgcgatcga
     4501 ccacagggcc cagttctgct ggatcgaagc caggacgcga gcaccggggt cccagccgtt
     4561 cggcgacgtc atcagggtgt agccgttgag caggaggatg acgaggggga agggcaccca
     4621 gatcgccttc tcggagttgg ccagtatcgc aatggacaac aggatcaccg cgaagaccag
     4681 ctgtacgacc accccgccaa ggaccccggg catcgtcgga agttgccagc tccacgagat
     4741 gagggggcgc agcccggcat acacaaggtt gacaaccgca gcgttgagaa cggcgtagcc
     4801 ggacatgaag aggaacttcg accagaagat ctcgtcgttg gtcagcggga gcgacttgag
     4861 gaaacggatc gtgccgctgg actcctcggt cgccaacagc cggtaggcca tcacgaacgt
     4921 gccgatggag gtcagacctt gtaggaaggc gcccgctgag gcgcgcatgg gttctgggaa
     4981 ctgggccacg aagctcagca gggtgaggat ctcgaacgtc accagaacga gaaaggcctt
     5041 ccactgcatt cgacagtctt tgaggatcag gacgctcagg ctcctgccgt caacgtgcat
     5101 gcccacctcc tcggcccatc gcaccttcca accgctcgcg ggcgagggca cggtcgccgg
     5161 cgatgtcgat ttgatcgatg atctgcccct gctcgagaag caccacccgg gaacagtgct
     5221 gggcaacgtc gtcgcgggag tgtgaggaga cgatcacggc cagcccggcc tcgaccgact
     5281 ggcgatccac cacctgccag atccattcgt tggaggcccc gtccaggcca gcagtgggct
     5341 cgtcgagcac aatgacgtcc ggccgtgggc taaagcccag gaccaggccg agcttcgtcc
     5401 gcatgccggc cgacaaatgc gccaccttgg tgtccaacgg aaggcgcaac tcgtcgcaca
     5461 gctgcgcgca ccagtcgtgg tcccagttgg ggtagagccg tcgggcgaag gcgaggaccg
     5521 caccgacggt cataccggca tacgtttggt tgttgtcgcc gacgaagcag acccgtcgct
     5581 tgaacgccct ttcatcggca cgatggtcga ggtcgcccag ggtgatcgag cccgatgtgg
     5641 gccggatcaa gtcggcaatg atacggaagg tcgtgctctt tcccgagccg ttgtgtccca
     5701 caagtccaac gacctcgccc ggtccgacgg cgaaggtcac cccggacagg acgaacccgc
     5761 caaggcgaac cccgactccg tcaagggaca gagtttcacg accgctgagc atgtgcaccg
     5821 ccttcgagga atacttgccg gcgcagggcc ttggtgtagc gccatgcgtt gagcagggcc
     5881 aacagaccca ggacgactcc gagaacaagg gcgagggagt tggagtacca cggcacaaaa
     5941 cgccgggcca gaccgtcgat gacgagcgca ccgacgacca gaccgccaac cgcgaaggcc
     6001 accgagagaa tcccgtagac caccaggaag gctcgctcct cggtggaggc gaccgccgga
     6061 gccgaccgcc gatgacccga gagcagggcg agcagcgtgc gcagcgcccg aggccgcaga
     6121 ttggggatgt gcatcaggtc gctcaatagc caatagccgt ccaaacggag gaacggattg
     6181 aggttgaaga tgatccgtgc cagcagtgcg tgcagcagca cgaagttcgc tgcggcgaag
     6241 gtcacgggca ggacgtcgaa cacccagagc agcacgcatc cggcaagcac gaacgactcg
     6301 acgtagatcc ccgcgaggtt gacgacgatc cggtgccggt tcttgctgaa cgcccaggcc
     6361 gcggacgtgt ccgtgtatgc cgccggggaa agcaggtaga gggcaagccc cattcggtgg
     6421 acctgtccgc cgaatcgcct ggtggcgagc gcgtgtccaa actcgtgcat cacggtcacg
     6481 accgcgttcg ccaggtagac cagcgcccag gcagagacca tcgcgaaggc ggtcatgtcc
     6541 gccacgtagc ggtcactgtg ctgcacgacc aaccacacgc accacgcgag cagggccaag
     6601 agcgtccccg cggccattcg ggagaacagc acccgcccga cgggagcaag tgcgtcgacg
     6661 acgcggtgcg ggtcgaattc ccccagcagg aggaacagga ggttgcctcg ctggggaccg
     6721 cgaaggtgcg aagggactgg cggtggctga ccgccctgga acagtcccag ttgatcagcc
     6781 cgttgagcca tggccgagaa ggtctgatcg ctcacgtgga ccccgatccc ggccagcgcg
     6841 gcatacacct ggttgtcatc acgcgaaccg tcgaacatcc gcagcacgcg caccgtcgcg
     6901 ccagtcatga ggtagtgcct gttggccacc ggatcggtca ccgtgacccg cccctcggcg
     6961 gtttcctcgc cgaggatcag gtcgtggcgc agcgtccggt gcacggtatc ggcctccttg
     7021 acggatcgtg tcgcgttcac gagaaggggt gggggtagga gttcaggtgg gtcaacagct
     7081 gttcttgcgt cccgtctgga cgctggccgc cgtgggtgtg cagccgacgg tgaccgaggt
     7141 aggggtacct gtgatccgcg ttcaggggca ccaactccgg gacgaagatc ttgacgacga
     7201 ccaggcccag ctcgtgcatg tcgcgggttg tgaggtcgac catcacgacg tccgagcagt
     7261 gttcttcgag ttgtcgcagg gtccggacca agtcccccgc ttggggcggc gcgaactccc
     7321 cgtcgatctg gtgcacctcg ctacttgaag tgaggaagtc gagggctagc tgcgcgcgtg
     7381 ggtcggtgta tgccctgccg tgccactcga aatccacgac gtcggagaag tcaggggccg
     7441 gcgtccacgg gggccgccga gggtccgcga gttcggtgcg cagccgctca tgatccgaga
     7501 gcgcctcaca gagcgccttg tcgcacgccc gtgacggaca gagtttggag gacaggccca
     7561 cggtggtcag cactcggccg agcgctgggc ggcgcacgac tgcgcacacc accggcaagc
     7621 cgaggtcggt cgtgagatca acgaatgtca cgctcgaaca tccatccgcc tcgacggcgg
     7681 cgacccgacg ggccaattcg ctgtccgggc gcggctccag ccgcggacgg accaactgac
     7741 ccatccaggt gatggcgaag gcatctctct cgacgacttc taggagcccg ctcaagcagg
     7801 catcgtccag cgtccaagcg gccgacatcc cggtcgagaa cgagggaccg atgacttcgt
     7861 ccggcgaggt gggtcgatag ggcaggtatg tgaaggaggc cgggatgtac cgccgttgac
     7921 cagtgaacag ggatcgcccc accgcccagt ggatgacgga gtcctgacga agcgggacat
     7981 aactgaagtc gcttcgcccg tattggaact ctgcgaaccg ttggatcgcg cagccgacgt
     8041 cgaaggcggg gccttccggt ttggacaaga gaagggcgga gtagtcgacg aacgcggcgc
     8101 agtagcgctc caccgcttcg aacgtcactt gcgagagaca ttggcgcagg tcccaggctg
     8161 aggcccccgg attggctatg tcaccgccag tggggagggt cccgtagctc gcaggcgcgg
     8221 cagcaaagtg cgcatacggc gcctggccac cgaccagcgg cagcagcaac gaccgcttca
     8281 agacccccgt gaccggcgag atgagctgat tggccaggtg cagggtgcgc tcgaacgcca
     8341 gatcggtcgt cactgctgct tctccgtccc catctcagcg cctcgggggc ccaggaggaa
     8401 acccaactgc accggctcgt cgacggggtt gacgccgagg aacgcggcga cctcgtcgtc
     8461 gtagatgccc cccaacgcgc aggtgtccag gcccatcgcc gtgccggaca ggtacagcga
     8521 ctgggtcagg gcaccgcagt cgtacatggc gaagcggtat ccgcggtgct ggtacttgga
     8581 catggttcgc ttcagaacac tggtcagcac gagggcgact gaactggtca ggcagaggcc
     8641 ctgataggag gacaacccag cgaactgggc ccgcaggtcc cccgaccgga tgagttcaag
     8701 ggaatgctcg atcgggcgat agtggtagag gccaggctca attcccgtca cgttgaaggc
     8761 caggacgtag atttcggtcg ggaagagcgc cccggccgag gtggtggccc ggaggtgttg
     8821 cacgtcacct gttcgagact cgatcttgga cgttggcccg taggccatga cgagggttcc
     8881 cccgagttgc tcggcggtga tcgcctcccc gacgaagtca cgccccagcg aactcaccga
     8941 ccggcgacgc agcacggtct cggcgaagcc gaccgtcgga gcgcggaagg gcggaagtgg
     9001 cgtgactgtc gagcccgggt aggacttcca gcttcgagcc atcatcgcga gcatcgcggg
     9061 gtccgacttg atctgctcca cccggtggct gatccgggcg acattcatcc gggtcaactt
     9121 ggtgtactcg ttgtaggtct cgctctcgga gtagtcgtcc tcttcgaaga agtccacgag
     9181 ggagtagctc tgcttgttca gcggctcggg catcgggctc tccttggtgc tgacgcggaa
     9241 cgcgctcggt gatcagggaa acggatgagg gactgggttg taacttcggt ggatgtcagc
     9301 gtcgaaccca agacggggtg ggactgtgtg gtagcggcgg gcgccgaagt tcggcagccc
     9361 gtgcacggaa tacagctgga cacttcccgg gatcaggaca cgcagcacca ccagccccgc
     9421 gtctgcgacg tcgcgggtcg tgatgtcgac gaccaaggcc tgcaggccaa ccgcctcaag
     9481 gcagtccaga acctgctccc gctgggcgcg aagcccagtg acgtccggcg cgggcgcgag
     9541 gccgtcccac tcgtcgacgt aaaggaagtc aaggtgttca agcatgcccg ggtcgccata
     9601 gaggcgcacg tggtcctcga agtcccggac atttgcgtag tcggggccgt gttcccagtg
     9661 gggcttggtg tcgatgaggt cgcgcaccca gacggccccc tgggcagcct cgacgatggc
     9721 cttgcggacc gcttccgact catcgagccg acaggacgcc cccacacagg cgaacgggcc
     9781 ttgatgatgg atgccacgcg cgacacacag cacagaaggt acggcgatgt ccgagcggag
     9841 ccgaaagacg tcgaaggtca gcgaggagtc tgcgaagtac ctgtcgaacc agggcccaac
     9901 cacggggtca tcacggtagt tcagccgggt cggcggcagt cgacgcaccc aggtgagcat
     9961 gaacgcgtcg cgctcgatca cctcatacaa accgctgagc agtgcaagat cggcgtcgct
    10021 gtgacaggcc tgccctgaag acaccgacag ggcaaccatg tcgctgtgtt cgacgagcct
    10081 tggcacgtag gggatgtaga ccaaacaggc cggaacgaag cgcggctcgc cgtctcggat
    10141 ccgcgcggcc ggcacccagg tcatcggaag gtcctcggta tgccgcgcga aagggaactg
    10201 cggatgcgcg tactggcgct cgtcgaagag ctcgaagtca tccacgagca gggcaccctc
    10261 cccgagttgc cgcgccggag cggtgaggag gttcggcggc tggacggcgc agcagtaccg
    10321 ctcgacacat tcgccgatcg cgctggctgc agcctgctcc caggtctcgg ccatcgctcc
    10381 cgtgtgtccg gccatctccg caccagcgat ggacgtcaac gagttcggga cgcttgcgac
    10441 actgaagagt cggggctcgc ccggagcgtt gaggatggtc cgcaccgcgc gcacgatcga
    10501 ggtgggcccc accatccagt ccagggcccc acccaagtcc tgacgtgatt caacgcccat
    10561 gtcgtcagct ccaagtcctc tcttgaggaa ctgaactcac ggtgctgcag tgcgggcacc
    10621 gaggcagctt gagcacgggg tgggtctcca ggcgcagctc ccacaggtcg aaggccagta
    10681 gcgatccggc ggttcgaacg aagacatcgc cggccgctag aagtcggaca ccttccagag
    10741 cgaccatcgc tccggtgaac gcggtgacgc tcggcagact gccgtgctcg acgtaccgct
    10801 ccagtgaagc gacgtagtcc tgaaactcct cgtaccgcac caccgaactg agattgctct
    10861 tgaaccggaa ctcaaagcac ttccaacacg ccgtctgccc agggatcacg gacggcccca
    10921 cgtggacgtt cgccctctgc aagagggcac cggtcgtcca cggcgtgccg gcggcgatgg
    10981 tcgcccgatt gaactcatcc atccacggca gcgacacgac cggggcatcc tgaacaacaa
    11041 cggcgacatc gatgtcgccc agcagttgct gccagcccaa cttgctctcc gggaatggcg
    11101 cgggctcgac gagggtggga aggtcattcc gcttcaagac cgccgcgacg acctcaccca
    11161 tggaccgatc cagatcggtg gggccgatgt gcgtggactg gccgacgtcg actgcaccca
    11221 cggtccgccg agcggcgacg acaatgcgtc gcacaccact ggccgcgagg cccacggcca
    11281 cgtcgggcat cagcggacct tcgccggcaa ggagaaccga gccagaggcg atccgtgcag
    11341 tcgtgccgtc atcgccgccg tgctggcgca tgaaccggtc ctggccccgc agcttcggca
    11401 ggagctgcga ggggaccccc tccaactcgg ccgcgggttc agcgaactcg acgaccccca
    11461 tgtccagcag ttgccgcagg ctggcctcca ggttggggaa ctccccgtcg gaggcgagtt
    11521 cggtcagcac cccttcgaaa tcccgcgtcc cgtccagcag gggaagcagt cgtgggaaga
    11581 cctccgagac gaggcggccc cgaaggactg cgctccgccg aggggtccgc atggtcaggc
    11641 ccccgtctgc cgcggggacg aggtagacct cccccaaccg aaggttgcct cgctgaggcg
    11701 cggatgcggt catggtcacg gcggaactcc ttgttggaca gcgggcgggg gagaatgcga
    11761 ggacggttcg ccgctgtggg gcacagaccg ctccacagcg gcgttccgct tgctcactca
    11821 gaaccagacg acgcaactgc acgacgtgca gccgcagctg gtggtgcacg cacatgcacc
    11881 accgaggagt tcagtccgct cctcaaggtc ttcgatctcg agcaggtccg tctcgttgat
    11941 ttccatccga ttcaccccct cccagggaca tccgccggcc tggatggtcg gctaccaggt
    12001 gtagacgcag gtgcaactcg tgcacgaaca gctgcacgag cacccgcacg accagctgcc
    12061 aggggtctcg cgctgctcaa gctcctcgat gctgaacatc tcgtcgacga gctcgacctc
    12121 ggtggaccga ttcatgtgtt actccaatca cgggacgaaa tggcacacgt tcaggaatgc
    12181 gcgaatggac ggttaggccc acgcaacgca acttgtggag gtgcagccgc aactgcaggt
    12241 gcaggcgcac acgccgctga tcaactctga tcgctcctcc agatcttcga tctgcaaggc
    12301 atcctcttcg aggacgatgt cgtgaaccat cgactccact atgtcacctc cttcctcggt
    12361 ctattactcc cgggtcacgg cgttgatttc atcgtcgatc tcagccagag atagagttcc
    12421 ggcaacaacc ttccgaatcg ttccggttcg gtcgatgaaa taccacgtcg gcgtgacgcg
    12481 caatgccagg taggtcgcga tcgaggagtc ggtgtcgacg atcacgggga acggcaccga
    12541 atccagggaa agggcctcgc tgggtggcca ggaaaggaac gggagatggc gagtggacac
    12601 gatcacgatg tcggccttgg gccggaaggt gcccgccgcg tgcccctgag acatgagcgc
    12661 gaggtcggcc ttgcagtact cgcaccaatg ggcgacgacg agcagaacga cggggcgccc
    12721 ggttggcact gagacgggct ggttggcgta gttgacaccc gaaatgctcg gtatgaccga
    12781 attgacctcc ccgccaggcc cggatccacc aaccatgatc ggatcgtcgg tgtgggtaag
    12841 cagtccgggc ttcatcccca gcgttccgct gaccaccgca aggccaagtc cgatgattgt
    12901 gacaaacagt actattcgga ccacgatgcg gcgtcgtcgc atcgtgcgct cggcttcggt
    12961 cacgtccccc cgtggactag acatggctat ccgccggcgg aggcaatgac gatggtcaac
    13021 tcactggcgg acggcaacgc tgtgtcatcg atattgatca cccgatatcc gtccgcaaat
    13081 atcagcgttg atcgcggcac ggtgcgatcc ggggccctca cgtatgcgct gacctcggga
    13141 agccggtcgc ataaatcgtc cagggctgca cggactgtgg cgccggaaca tcgaacctgg
    13201 ggtggtagtc catatcgcgc ccacacagct tggggcaggc agatgtcgtg ctcgtccatg
    13261 gacgaaacct ctgtcatgta tcggggggag atcctctacg ctgccaacgt aacacggcac
    13321 cgttggaccc gcaacgcgaa tcggaaagcg gccgaactac acccgcgcga cacatcagtg
    13381 tttggtccgc cgaaactgtc ggaacgtgta atgatgcgca taaacggcgg cctcggggtc
    13441 gcacattcgt gatggcgcac cgccgtcggc ggaccgcgag gtgcccgacc tgcgactcag
    13501 cgggagtcgg cccggtcgtc gggggtagcc gggtcgaccg gcaacgggcc cacggcatac
    13561 agggggtcgt cctcgctgcc ggccaggatc gaacggaacg cttcgaggtt ggccaacgac
    13621 tcaccgcgag cgacccgcca ggcccactcg cgcttcatcg acgtgagaaa gccgatcgcc
    13681 agcaggtcgt tgaacggctc atcggcggcc tccagcagcg cccccaccag cagatccaga
    13741 taccgcccat ccagcgccac cgtcggcacc tggccacgga catagacgtc accagagccg
    13801 tcgacggcat accccagcag ggggcgacgc atcacccgcc gcagcagga
//
LOCUS       DAORPW010000052        13819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_892_length_13819_cov_729.642982, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000052 DAORPW010000000
VERSION     DAORPW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13819
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_13819_cov_729.642982"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1076
                     /locus_tag="PLB86_06195"
     CDS             129..1076
                     /locus_tag="PLB86_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HRC64761.1"
                     /translation="MDTTTFTVTATDGTPLHVFRWLPDAGAAPKAVVQIAHGMAEHSA
                     RYARFAEVLTAAGYAVYAKDHRGHGQTAGDLKRAGFFAEHDGWSTVVEDMYAVTRTIR
                     EEQPGLPIFLFGHSMGSMLSRTYAIRHGAELDGLILSGTGGDPGMLGKVGQVVALVEA
                     KVRGKGTASPLLDKLSFGNFNKPFEPARTPFEWLSRDPAEVDAYIADPWCGFVCTAGM
                     FQDLLSGIALINADSQVARIPQGLPIYLFSGAEDPVGDKTKGVQQVVDQFRRLGIKDV
                     FVRFYLGARHEVLNETNRDEVMADVVDWLDDHLSVTERA"
     gene            complement(1060..1584)
                     /locus_tag="PLB86_06200"
     CDS             complement(1060..1584)
                     /locus_tag="PLB86_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC64762.1"
                     /translation="MSDHTSSREHATTGTMEGERAPDAPLPHTAFVNQWRSGDTLLTL
                     RELLDLAEEVGPTIARRAELGHTELKAIELLVRSPHGPVELARELGVTSAASSGIVDR
                     LEARGHVRREAHATDGRRTQVVLTESAREEVLGHLVPMFGALAAMDATFTEDEKVIVQ
                     RYLRGAIGAIKRVL"
     gene            1645..3678
                     /locus_tag="PLB86_06205"
     CDS             1645..3678
                     /locus_tag="PLB86_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRC64763.1"
                     /translation="MTRLGQAIVDLIVHRRRSWLVALLLVLVAGGVIGAMKEATRTTS
                     ALDGLPIGAESTAVVEVLQTFPQKEGSSAVVLYAATNGTLDEPTIAALRTRLQGRIAG
                     PLVVSEDKSAAIGVVPVTATGNAGISAAVTSLRTDLKADPPAGVTVQVTGPAAVQADL
                     GAVFQGANTRLLLATAGVVALLLLITYRSPTLWLVPLIVVGIADRLAAVLATHVLKAT
                     GVAWDESTIGILSVLVFGAGTDYALLLISRYRDELRREPDRYAAMRAALRHTGEAVLL
                     SATTVVLGVLTLLLSVFPTTRGLGLASAVGIVVAAGYALVVLPAVLVLFGRWVFWPVV
                     PRVGQTALVDQNSFWRKVGDLVAARPQRFVVGTLVVLALMAGGLTQVRSGLSQTDQFL
                     SKPEAIAAAERLAQSFPAGNSDPLVVITEGDPAAVVAAAKSVPSVASATPGQRTDRYA
                     QVAVVLTPQPGTDAARTAVRDVRAAVHGIPGTIVGGTEARGVDAADAASRDRGVIFPI
                     IFVLVLLALLVLLRSVVAPIILVGTVILTFLAAVGASWWIFTKVFGFTALDVGAPLYT
                     FLFLVALGVDYNIFLVTRAREEAAVHGSRAGMLRALSATGGVITSAGILLAAVFAVLG
                     VLPLVVLAQIGVIICVGVLLDTLVVRTVLVPAIALILGDRFWWPRQVAPVERA"
     gene            3708..4976
                     /locus_tag="PLB86_06210"
     CDS             3708..4976
                     /locus_tag="PLB86_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC64764.1"
                     /translation="MTSARRERVAVVGSGVSGLTAAYLLSRAHDVTLFEADDRLGGHA
                     HTHSVAGSSGPVAVDSGFIVFNDRTYPVLRRLFAELGVSGRPTEMSMSVRDDRSGIEF
                     AGGRGLPGFVARPGQLARGSYWRMLTGVRRFHRLARVFLEGSTDEDPTTFGEFIRAAG
                     FGPDFVRLYAVPVVACVWSTGGGDALDYPARYLFRFLDHHGMLSIGDSPQWFTVVGGS
                     RTYVDAIAARLPDVRSGRPVRVVQRHADGVSVIDASGGRTEVDRVVIATHPDQALSLL
                     ADPGPLEASLLGSFRYTTNEAVLHRDPSLLPTASGAKASWNYLVQGDDDGPPLVTYWM
                     NRLQGLPSTDQLFVTLNGSDRIHPESVIATMSYAHPLYDLAAVRAQRRLPEISSGRTA
                     FAGAYHGWGFHEDGARSGVAAAAALGVTWG"
     gene            4981..6975
                     /locus_tag="PLB86_06215"
     CDS             4981..6975
                     /locus_tag="PLB86_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HRC64765.1"
                     /translation="MTEPLLPVPALVVGTVRHTRFRPLRASFAHRHYQWLVDLDHPPR
                     LPARLRRLSELRAEDHLDGRASTLAELKAIVLQEVRAGGVELPDSTRVLLLAHARVFG
                     HVFDPLSVFWCLAPDGRVVAAILEVRNTYAGRHTYVLTPDPQGKAEVDKDFLVSPFND
                     TTGRYAVRLHLTRDRVSVAIRLERNGAQLLSATVTGRPVPLTGASLTRVLVRHPLMPQ
                     RVSALIRVHGVALWLRRLPVLREKPPSSADAQAASGVDLRIAPAGRHTRVTRGFVARN
                     LVRLLVRSLPIQLRLPGGGTWGAGGANALVLQVNRPAALFRRLETHPKIGLGEAFMAG
                     DWEAGEGTDLADLLRPFAARVDRLLPRPVLALRGIVERAIPHHHRNTPDGARANIAAH
                     YDLSNDLFAGFLDETMTYSSALFEGSRPWETQDLATAQRRKIDAALDAANVRAGTRML
                     EIGTGWGELALRAAARGASVTTITLSREQQALARQRVLDAGLADRVDVQLRDYREVTG
                     QFDAVVSIEMIEAVGEEFWPAYVETIDRVLAPGGSAVVQSILMDHDRFLATRNSFGWI
                     QKHIFPGGIIPSLEALEEVATRHTSLRVKGIRTFGAHYAHTLRRWRQTFTDNWPQIAT
                     HGFDDVFRREWEFYLAYCEAGFATGYLDVAQITFGRSGQS"
     gene            6972..7958
                     /locus_tag="PLB86_06220"
     CDS             6972..7958
                     /locus_tag="PLB86_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC64766.1"
                     /translation="MTHPTWTDRLARLVDTALDRSVVLGYPATGHAVRRRLPTWPKDP
                     PRNALSGKHVLVTGASSGLGVRTAVDLAMLGAHVHLVVRDPMKAAPVREQLDEVAAGR
                     HTTWRCDLSDQASVRAFAAAYRATGLPVDGLVHNAGIMPSTRQEDAQGRELAMSVHVL
                     GPVALTEALLPALAAARDGARVVFVTSGGMYSQALDLDDLDFRPEPYRGAVAYARSKR
                     AQVELLPILARRWGRHRISVYAAHPGWAASPGVTTSLPAFARLTAPVLRRGAAGVDTT
                     TWLIAATPAPPSGGLWHDRSERPTSYLSRTRASDAERQELWQWVARHAGIAD"
     assembly_gap    8001..8100
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8200..11778)
                     /locus_tag="PLB86_06225"
     CDS             complement(8200..11778)
                     /locus_tag="PLB86_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRC64767.1"
                     /translation="MTRLTKLSLANRTVVLLVCAILIGLGLFATSSLKQELIPSIETP
                     AATIVSVLPGAAPEVVEREVSRPIEDAAKAVSGITRVTSRSASAVSTVRVEWEFGTGS
                     DKVLADLRSAVDGIQGRLPSDVTPQVVAGRFDDVPIIVLAVSSTESKAILSGKLKDIV
                     AAKLKTLPGVRDVVVSGQEQEQVVVTLRPADVDRLGVDTSTLAQVFAASGIAVPAGSI
                     PSAGTTMDVEVGKTLTSTDAIAAIQLQGTDGPLALKDIADVTMAPVETTSISRANGKP
                     SLTVAVTKEQSGNTVAVSQAVRAELPALMARVGGGATDSIVFDQAPYIEDSVHDLTVE
                     GLLGLFFAVIVILIFLRSVRSTIITAISIPLSLLIAMIGLWTGNYTLNILTLGALTVA
                     IGRVVDDSIVVIENIKRHQGLGEFGPASIIRAVREVAGAVTSSTLTTVAVFLPIGLVS
                     GQTGELFRPFAMTVTIALLASLFVSLTVVPVLASWFMRGRGASAAAEGDDAAYQSAGM
                     PLGSSESPRTGLQRAYLPTLRWALDHRVVTLVLAALVFGGTVGLAPRLKTDFLGSMGA
                     TTLRVVQEMPAGTALDQTDAAAKRLEQVIGADPTVVSYTTTVGGDPSRVFFGTVAGPN
                     QASISVTLSSGSDGVVVGDRLRSALTALSDVGTVEVVSTQSNSDVALVVEGSDPDSLR
                     KGSDQVAEMLASVPGMRNVRTDLTNAKTMLKVDVDAVAAANVGMVQAQIGQAVTQAIR
                     GTKVGTVTVDDTTVDVILRSREPVTTVEGLRALPLPVTQKQTIDARKAAGTTVEDDQK
                     AMQKQAQADQDAAVASQLSGLRESRTKVSETISSLTSQLAALNAQLAQLSALPPGTVT
                     APPVDPIATLRAQIAQLSKSIETAKAQLTATDESIDKVLESKAKTAQSRADSEALSQA
                     AKDAQKAKATPVLLDAVADVTEVSSPASIGRVDGARAATVNGTPQAGDVGGVTSTIQT
                     KLAALALPEGVTVRLAGVSQAQRESFSQLGVAMLAAIAIVYLIMVATFRSLLQPLILL
                     VSVPFAATGALALLLITGTALGIPAMIGLLMLIGIVVTNAIVLIDLINQYREGGAGIS
                     DSIINGARLRLRPIIMTALATIFALLPMGLGVTGGGIFISKPLAIVVIGGLVSSTVLT
                     LVLVPVLYDIVERTRERVRQRRGGGTEGRVATEGAVTEGAVAGVTA"
     gene            complement(11854..13188)
                     /locus_tag="PLB86_06230"
     CDS             complement(11854..13188)
                     /locus_tag="PLB86_06230"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRC64768.1"
                     /translation="MIGSGFGGLFVTKALRKADVEITLIDRTSHHLFQPLLYQVATGI
                     LSEGEIAPSTREILRRQKNVRVVQGLVETIDLESKTIGWRFHDEERTSAYDSLVIASG
                     AGQSYFGNPQYATFAPGMKSIDDALELRARIFAAFELAEIADDPEEVSRLLTFVVVGA
                     GPTGVEMAGQIRELANRTLAREFRRIDPTKARVILVDGAGQVLSAFGEQLGAKAERDL
                     SRLGVDVVLHAMVTSLDATGLTLRHADGSTERIEAVCKVWAAGVEGSPLGRLVADQAG
                     ATVDRAGRVEVNDHLTLPGHPEVYVIGDLMSLPGVPGVAPAAIQSAQYAAASIAARVE
                     DRPAPGPFAYKDKGSMATIAKYEAVVRVGPLRLTGFLAWLAWLLLHLLMIAGFKQQVS
                     TLVRWALSFLSNGRSERVTTNQQLVGRLAMEHLGGHTSGPLMRGEVPPDPVD"
     gene            complement(13307..>13819)
                     /locus_tag="PLB86_06235"
     CDS             complement(13307..>13819)
                     /locus_tag="PLB86_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC64769.1"
                     /translation="AIAEEQGHHPEIDLRWGRVHIATGSHDVGGVTERDIRLGKAIDA
                     VVQEMDLTSQPGRLTEVEIAIDAMDRDAIRPFWAAVLDRPDNGGDELVDPDRVGPAVW
                     FQQMDSPRPQRNRIHLDVTVSHDEAPGRLARALAAGGRLVSDVRAPAFWVLADAEGNE
                     ACICTWQSRD"
BASE COUNT         1973 a         4793 c         4711 g         2242 t
ORIGIN      
        1 ccgatgtcga tgtgctcatg cggtgcatgc aagatggtct cgacgaggtt ctcgctgtgg
       61 cgacgtcgtg acgtcgtggc ggcatgacgg cgcaggaccc cgcgaactcg atgcctgagg
      121 aggcaccaat ggacaccacc acgttcaccg tcacggccac cgacgggacg ccgctgcacg
      181 tcttccgctg gctgcctgac gcgggcgccg cgcccaaggc ggttgtccag atcgcccacg
      241 gcatggctga gcactccgcg cgctacgccc ggttcgccga ggtgctcacc gccgccgggt
      301 atgccgtcta cgccaaggac caccgcggcc acgggcagac cgccggcgac ctcaagcggg
      361 cggggttctt cgcggagcac gacgggtggt cgacggtggt cgaggacatg tatgccgtga
      421 cccgcaccat ccgcgaagag cagccgggcc tgccgatctt cctcttcggt cacagcatgg
      481 gctcgatgct cagccggacc tacgccatcc gccacggggc ggagcttgat gggctgatcc
      541 tctcgggcac cggcggtgac ccggggatgc tcggcaaggt cggtcaggtc gtggcgctcg
      601 tcgaggccaa ggtccgcggc aagggcacgg cgagcccgct gctggacaaa ctgtccttcg
      661 gcaacttcaa caagcccttc gaacccgcgc gcacgccgtt cgagtggctg tcccgtgacc
      721 ccgccgaagt ggacgcctac atcgccgacc cctggtgtgg attcgtctgc acggcaggca
      781 tgttccagga cctgctcagc ggcatcgcgt tgatcaacgc cgacagccag gtcgcgcgga
      841 tcccgcaagg cctgccgatc tacctgttct ccggcgccga ggacccggtg ggagacaaga
      901 ccaagggcgt ccagcaggtc gtcgaccagt tccgccggtt gggcatcaag gatgtcttcg
      961 tgcggttcta cctcggcgcc cgccatgagg tgctcaacga gaccaaccgc gacgaggtca
     1021 tggccgacgt cgtcgactgg ctcgacgacc acctgagcgt cacagaacgc gcttgatcgc
     1081 gccgatcgcg ccgcgcaggt agcgctgcac gatcaccttc tcgtcttcgg tgaaggtcgc
     1141 gtccatcgcc gcgagggcgc cgaacatcgg gacgaggtgc ccgagcacct cctcgcgtgc
     1201 tgactcggtg agcaccacct gggtgcggcg accgtcggtg gcgtgagcct cccggcggac
     1261 atggccgcgg gcttcgagcc ggtcgacgat gccggaggat gccgcagagg tgacccccaa
     1321 ctcgcgggcc aactcgaccg ggccatgcgg tgaccgcacg agcaactcga tcgccttgag
     1381 ctcggtgtgc ccgagctcgg cgcgtcgggc gatggtcggg ccgacctcct cggcgaggtc
     1441 gagcaactcg cgcagggtga ggagggtgtc gccgctgcgc cattgattga cgaacgccgt
     1501 gtggggaagc ggcgcgtccg gcgcccgctc cccttccatg gtgccggtcg tcgcatgctc
     1561 ccggcttgac gtatgatcgc tcattagatt actaatctac ttacttagtt cgtgaagtac
     1621 cagactagcc gatcggattg tcccatgact cgtctcggtc aagccattgt cgacctcatc
     1681 gtgcatcgac gtcgctcgtg gctcgtggcc ctgctcctcg tgctcgttgc gggtggcgtc
     1741 atcggtgcca tgaaggaggc gacccgcacg acgagcgcgc tcgacggatt gccgatcggc
     1801 gccgagagca ccgcggtcgt ggaggtgctg cagaccttcc cccagaagga gggcagcagc
     1861 gccgtcgttc tctacgccgc gaccaacggc accctggacg agccgacgat cgccgccctg
     1921 cgcacccgcc tgcaaggcag gatcgccggt ccgctcgtgg tcagcgagga caagagcgcc
     1981 gcgatcgggg tcgtcccggt cacggcgacg ggcaacgccg ggatctcggc ggccgtcacc
     2041 tccctgcgca ccgacctcaa ggccgacccg ccggcggggg tcaccgtcca ggtcaccggc
     2101 cccgcggcgg tccaggccga cctcggtgcc gtcttccagg gcgccaacac caggctgctc
     2161 ctcgccacgg ccggcgtggt cgccctcctg ctgctcatca cctatcgcag cccgacgctc
     2221 tggctcgtcc ccctcatcgt cgtcgggatc gccgaccggc tcgccgcggt cctcgccacg
     2281 cacgtcctca aggccaccgg cgtcgcgtgg gacgagtcga cgatcggcat cctctcggtc
     2341 ctcgtcttcg gcgcgggcac cgactacgcc ctccttctca tctcgcgcta ccgcgacgag
     2401 ttgcgccgcg agcccgatcg gtatgccgcg atgcgcgcgg ctctgcggca taccggagaa
     2461 gcggtcctgc tcagcgcgac gacggtcgtg ctcggcgtcc tcaccctgct gttgtcggtc
     2521 ttcccgacga cgcgcgggct cggtctggcg agcgccgtcg gcatcgtcgt cgccgccgga
     2581 tatgccctcg tcgttctccc cgcagtgctc gtgctcttcg gccgctgggt gttctggccg
     2641 gtcgtgccgc gagtcgggca gacagccctc gtcgaccaga actcgttctg gcgcaaggtc
     2701 ggcgacctgg tcgctgcgcg gccgcagcgg ttcgtcgtcg ggaccctcgt cgtcctggcg
     2761 ctgatggccg gtgggctcac gcaggtccgc agtggcctct cgcagaccga ccagttcctc
     2821 tccaagccgg aggcgatcgc ggctgccgag cggttggccc agtccttccc ggccggcaac
     2881 tccgacccgc tcgtcgtcat cactgagggc gacccggccg ctgtcgtggc ggccgccaaa
     2941 tccgtcccgt cggtcgcctc cgcgaccccc ggccagcgca ccgatcgcta cgcccaggtg
     3001 gccgtggtcc tcacgcccca gccagggacc gacgcggcgc gcaccgcggt gcgggacgtg
     3061 cgcgcggcgg tgcacggcat accgggaacg atcgtcggtg gcacggaggc gaggggcgtc
     3121 gatgccgcgg acgccgcgtc gcgcgaccgg ggcgtcatct tcccgatcat cttcgtcttg
     3181 gtgctgctcg ccctgctcgt gctgctgcgt tcggtcgtcg caccgatcat cctcgtcggg
     3241 acggtgatcc tcaccttcct cgcggccgtg ggggcgtcct ggtggatctt caccaaggtg
     3301 ttcggcttca ccgcgttgga tgtcggggcg ccgctctata cgttcctgtt cctcgtggcg
     3361 ctcggcgtcg actacaacat cttcctcgtc acgcgggcgc gggaggaggc ggccgtccac
     3421 ggctcgcggg cgggcatgtt gcgggcgctg tcggccaccg gcggcgtcat caccagcgcg
     3481 ggcatcctgc tggctgcggt gttcgccgtc ctcggggtgc tgcccttggt cgtcctcgcc
     3541 cagatcggcg tcatcatctg cgtcggcgtg ctgctggaca ccctcgtcgt gcgcaccgtc
     3601 ctcgtcccgg ccatcgcgtt gatcctcggg gaccggttct ggtggccgcg acaggtggct
     3661 ccggtggagc gagcgtgagt ggtgacccgg ccagtcccgg cgatctcatg acgtccgcac
     3721 gccgggagcg ggtcgcggtc gtcggctccg gggtctcggg gctcaccgcg gcatacctgc
     3781 tctcccgggc ccacgacgtg accctcttcg aggccgatga tcggctgggt ggacacgcgc
     3841 acacgcactc ggtcgccggg tcctcaggac cggtggcggt cgactcgggc ttcatcgtct
     3901 tcaacgaccg gacctatccc gttctgcgtc ggctcttcgc cgagctcggg gtcagcgggc
     3961 ggccgaccga gatgtcgatg agcgtgcgtg atgaccggtc tggcatcgaa ttcgccggcg
     4021 gcagagggct tcccgggttt gtcgctcggc cggggcagtt ggcgcggggc tcctattggc
     4081 ggatgctgac gggggtccgg cggttccacc ggctggcccg ggtgttcctt gagggcagca
     4141 ccgacgagga tcccacgacg ttcggtgagt tcatccgcgc cgcgggattc ggtcccgact
     4201 tcgtccgcct gtatgccgtg cccgtcgtcg cgtgtgtctg gtccactggg ggaggggacg
     4261 cgctcgacta cccggcccgc tacctgttcc gattcctcga ccaccacggg atgctgtcga
     4321 tcggggactc gccgcagtgg ttcacggtgg tcggtgggtc gcggacctat gtcgacgcga
     4381 tcgccgcgcg attgccggac gtccgctccg ggcggccggt ccgggtcgtg cagcggcatg
     4441 ctgacggggt ctcggtgatc gatgcgtcgg gaggccgcac cgaggtcgac cgggtcgtca
     4501 tcgccaccca ccccgatcag gcgctgtcgt tgctggctga cccagggccg ttggaggctt
     4561 cgctgctcgg ctcgttccgg tacaccacga acgaggctgt cctgcatcgc gatccgtccc
     4621 tgctgccgac cgcgtccggg gcgaaagcgt cgtggaacta cctcgtccag ggcgacgacg
     4681 atgggccgcc attggtcacc tactggatga accgcctgca gggtctgccg agcaccgacc
     4741 agttgttcgt caccctcaac ggttccgacc ggatccaccc cgagagtgtc attgcgacga
     4801 tgtcctacgc ccaccctctc tatgacctag cggcggttcg cgcacagcgc cggctgcctg
     4861 agatcagcag cggcagaacg gcattcgccg gggcctatca cgggtggggg ttccacgagg
     4921 atggcgcccg ctccggcgtg gccgccgcgg cggctctcgg ggtgacgtgg gggtgacctg
     4981 gtgactgagc ccctcctgcc ggtgccggcg ctcgtcgtcg gcaccgtgcg gcatacccgc
     5041 ttccgcccct tgcgggcttc gttcgcccac cggcactacc aatggctcgt cgacctcgac
     5101 cacccaccgc gcctgccggc ccggctgcgt cgcctgtccg aactgcgcgc cgaggaccat
     5161 ctcgatggtc gcgcgtcgac cctggcggag ctgaaagcca ttgtcctgca ggaggttcgc
     5221 gccggtgggg tcgagctgcc cgactcgaca agggtgttgc tccttgctca tgcccgagtg
     5281 ttcggacacg tctttgaccc cttgtcggtc ttctggtgtc tggcgccgga cgggcgggtc
     5341 gtcgcggcca ttctggaggt ccgcaacacc tacgccgggc gccacaccta cgtcctgacc
     5401 cccgacccgc agggcaaggc cgaggtcgac aaggacttcc tcgtctcgcc gttcaacgac
     5461 accaccggca ggtatgccgt gcgcctgcac ctcacgcgcg atcgggtgtc ggtggcgatc
     5521 cgcttggagc gcaacggtgc tcaactcctg agcgccaccg tcacaggtcg cccggtcccg
     5581 ctgaccgggg cctccttgac ccgggtcctt gtccggcacc cgttgatgcc gcagcgggtg
     5641 agcgccctga tccgggtcca cggtgttgcc ctctggttgc gccgactgcc ggtcctgcgt
     5701 gagaagccgc cgagcagcgc ggacgctcag gctgcctctg gggtagacct gcggatcgcg
     5761 ccggcagggc ggcatacacg ggtcacgcgt gggtttgtgg cccgcaacct ggtccgcctg
     5821 ctggtgcgca gcctccctat ccagctgcgt cttccgggcg gtggcacgtg gggcgcgggc
     5881 ggtgcgaatg ctcttgtgct gcaagtcaac cggccggccg cgctgttccg gcgactcgag
     5941 acccacccga agatcggcct cggcgaggcc ttcatggcgg gtgactggga ggcgggcgaa
     6001 ggaaccgatc tggccgacct cctcaggccg ttcgccgcgc gggtggaccg gcttttgccg
     6061 cgaccggtcc tggcgctgcg cggcattgtt gagcgggcga tcccgcacca ccaccgcaac
     6121 accccggacg gcgcgcgcgc caacatcgcc gctcactacg acctgagcaa cgacctgttc
     6181 gcgggcttcc tcgacgagac gatgacctac agcagtgcgc tgttcgaggg ctccaggccg
     6241 tgggaaacgc aggatctcgc gactgcccaa cgccgcaaga tcgacgcagc cctcgatgcc
     6301 gccaacgtcc gggcgggcac gcggatgctc gaaatcggca cgggttgggg cgaattggcg
     6361 ttgcgggctg cggcccgagg cgcgtcggtc acgacgatca ccctgtctcg ggagcagcag
     6421 gcgctggctc ggcagcgggt gctggacgcc gggctagccg accgggtgga cgtgcagttg
     6481 cgcgactacc gcgaggtgac cgggcagttc gacgcggtcg tcagcatcga gatgatcgag
     6541 gcggtcggcg aggagttttg gcccgcctac gttgaaacga tcgaccgagt gctggcgcct
     6601 gggggatcgg cagtcgtcca gtcgatcctc atggatcacg accgctttct cgcgacccgc
     6661 aactcgttcg gctggatcca gaagcacatc ttcccgggcg ggatcatccc atcgctggag
     6721 gcgctcgagg aggtggctac gcggcatacc tcgttgcggg tcaaggggat tcgcacgttc
     6781 ggtgcgcact atgcccacac cttgcggcgg tggcggcaga ccttcaccga caactggccg
     6841 cagatcgcca cccacggctt cgacgacgtc ttccgccgtg agtgggagtt ctatctcgcc
     6901 tactgcgagg cggggttcgc caccggctac ctcgacgtcg cgcagatcac tttcggcagg
     6961 agcggacagt catgacgcac ccgacgtgga ccgaccggct ggcccggctc gttgacacgg
     7021 cgctggatcg gagcgtcgtg ctcggctacc cggcgaccgg ccacgccgtg cgccggcgac
     7081 tgccgacctg gccgaaagac ccgccccgca acgctctgag cggcaagcac gtcctggtca
     7141 ctggggcgtc ctcgggcctg ggggtccgaa cggccgtgga tctcgcaatg ctcggtgccc
     7201 acgtgcacct tgtcgtgcgc gacccgatga aggccgcccc ggtccgcgag caactcgacg
     7261 aggtggcggc gggacggcat accacctggc ggtgtgacct ctccgatcag gcgagcgtgc
     7321 gtgcgttcgc ggcggcctat cgcgcgacgg ggctgccggt cgacggactg gtccacaacg
     7381 ccgggatcat gccgtcgacg cgccaggagg acgcgcaggg tcgggagcta gcgatgtcgg
     7441 tgcacgtcct cggcccggtc gcgttgaccg aggcgcttct gcccgccctt gccgccgctc
     7501 gtgacggcgc gcgggtcgtg ttcgtcacct ccggcgggat gtactcgcag gcgctggacc
     7561 tggacgacct ggacttccgc cccgagccct atcgcggcgc ggtcgcttac gcgcgcagca
     7621 agcgggccca ggtggagttg ctcccgatcc tcgcccgccg ctggggtcgc caccgcatca
     7681 gcgtgtatgc cgcccaccca ggctgggcgg ccagcccggg tgtcaccacc tcgctgccgg
     7741 ccttcgcacg gctgaccgct ccggtgctgc gccgcggcgc ggccggtgtc gacacgacga
     7801 cctggctcat cgcggccacc cccgcgccgc cgtcaggggg cttgtggcac gaccggtctg
     7861 agcgtccgac gagctatctg tcccgcaccc gggccagcga tgccgagcgc caggagttgt
     7921 ggcagtgggt cgcccggcac gcaggcatcg ccgactgaat cgcccggccg ccaaacgtgg
     7981 acttcggcca tgtcgtacga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 tgccaatggt cgccacccgc tcgccctccc acgctcaggt atgccgcccg ctccccacca
     8161 atggccttcc ctgtgcgctg cccgcaactc cgacggctgt caggccgtga ccccggcgac
     8221 agcgccctca gtgacggcgc cctcggtggc gacccgcccc tcagtcccgc cgccccgacg
     8281 ctgtcggacc cgctcgcggg tgcgctcgac gatgtcgtag agcaccggca cgagcacgag
     8341 ggtgagcacg gtggaagaca ccaatccgcc gatgacgacg atagccagcg gcttggagat
     8401 gaagatcccg ccgccggtca cgcccagacc catcggcagc agcgcgaaga tcgtcgccag
     8461 cgcggtcatg atgatcggcc gcaaccgcaa ccgggcgccg ttgatgatcg agtcgctgat
     8521 ccccgcgccg ccctcgcgat actggttgat caggtcgatg aggacgatgg cgttggtcac
     8581 gacgatgccg atgagcatga gcaggccgat catcgccggg atccccaacg cggtccccgt
     8641 gatcaacaac agcgccaaag cgccagtggc tgcgaacggc accgacacca aaaggatgag
     8701 cggctgcagc agcgagcgga acgtcgccac catgatgagg tagacgatcg cgatcgccgc
     8761 gagcatcgca acacccaact gggagaacga ctctcgctgg gcctgagaga cacccgccaa
     8821 ccgcaccgtc acgccttccg gcagagcaag ggcagcgagc ttggtctgga tcgtcgaggt
     8881 cactccgccg acatcacctg cctgcggtgt cccgttgacg gtggcggcgc gggccccgtc
     8941 gacccggccg atcgaggctg gcgaggagac ctcggtgacg tcggcgacgg cgtcgagcag
     9001 gaccggagtc gccttcgcct tctgggcgtc cttggccgcc tggctcaggg cctcgctgtc
     9061 cgcgcggctc tgtgcggtct tggccttgct ctccagcacc ttgtcgatgc tctcgtcggt
     9121 cgcggtcagt tgcgccttgg ccgtctcgat cgacttgctc agctgggcga tctgggcccg
     9181 gagggtggcg atcgggtcga cggggggcgc cgtcaccgtt cccggcggaa gggccgagag
     9241 ctgcgccaat tgcgcgttga gcgcggcgag ttggctcgtc agcgagctga tggtctccga
     9301 caccttggtc cgggactcac gcaggcccga cagctgcgac gccactgccg catcctggtc
     9361 ggcctgggcc tgcttctgca tcgccttctg gtcgtcctcg accgtcgtgc cggcagcctt
     9421 gcgggcgtcg atggtctgct tctgggtgac cgggagcggg agcgcccgca gaccctcgac
     9481 cgtggtcacc ggctcgcgcg agcgcaggat gacatcgacc gtcgtgtcat ccacggtgac
     9541 cgtgccgacc ttggtgcccc tgatcgcttg ggtgacggcc tgcccgatct gggcctgcac
     9601 catgccgacg ttggcggcag cgaccgcatc gacgtcgacc ttgagcatcg tcttggcgtt
     9661 ggtgaggtcg gtgcgcacgt tgcgcatccc cggcacgctc gcgagcatct cggcgacctg
     9721 gtcgctgccc ttgcgcaggg agtccgggtc agacccctcg accacgaggg ccacgtcgct
     9781 gttggactgg gtggagacga cctcgaccgt gccgacgtcg ctgagggcag tgagggcaga
     9841 gcgcagccgg tcgcccacga cgacaccgtc cgagccagag gagagcgtca ccgagatcga
     9901 cgcctggtta gggccggcga cggtcccgaa gaacactcgg gaagggtcac cgcccaccgt
     9961 cgtcgtgtag gacacgaccg tcggatcggc gccgatgacc tgctcaagtc gcttggcggc
    10021 agcgtcggtc tggtccagcg cagtgcccgc cggcatctcc tggacgaccc gcagcgtcgt
    10081 tgctcccatg ctcccgagga agtcggtctt cagccgcggg gccagcccga ccgtgccgcc
    10141 gaacaccagc gcggcgagca cgagcgtgac gacgcggtgg tccagcgccc accgcaaggt
    10201 cggcaggtag gcccgctgca gcccggtccg cggcgactcg gaggagccga gcggcatacc
    10261 tgcgctctgg tatgccgcgt cgtcaccttc cgcagccgcg ctcgctcccc ggccgcgcat
    10321 gaaccacgac gcgaggaccg ggaccacggt gagggagacg aacaacgatg ccagcagcgc
    10381 gatggtgacg gtcatggcga acggccggaa caactcgccg gtctggcccg acaccaaccc
    10441 gatcgggagg aagacggcga ccgtcgtcaa ggtcgaggac gtgacggccc ctgcgacctc
    10501 gcggaccgcg cggatgatcg acgcggggcc gaattcgcca agcccctgat gccgtttgat
    10561 gttctcgatg acgacgattg agtcgtcgac gacccggccg atcgccacgg tgagggcacc
    10621 gagggtgagg atgttgaggg tgtagttgcc ggtccagaga ccgatcatcg cgatgagcag
    10681 cgacagcggg atggagatcg ccgtgatgat cgtcgagcgc accgaccgca ggaagatgag
    10741 gatgacgatg accgcgaaga agaggcccag caggccctcg acggtcaggt catggacgga
    10801 gtcctcgata tagggcgcct ggtcgaacac gatcgagtcg gtcgctcccc cgccgacgcg
    10861 cgccatgagg gcgggcaact cggcccgcac ggcctgcgac acggcaacgg tgttgccgct
    10921 ctgttccttg gtcacggcga ccgtcaggga cggcttgcca ttggcgcgcg agatcgacgt
    10981 cgtctcgacc ggggccatcg tcacatcggc gatgtccttg agggccaacg gtccgtcggt
    11041 gccctgcagt tggatggcgg cgatcgcgtc ggtgctggtg agggtcttgc cgacctcgac
    11101 atccatcgtg gttccggctg acgggatcga cccggcgggc acggcgatgc cgctggccgc
    11161 gaagacctgg gcaagcgtcg aggtgtcgac gccgaggcgg tcgacgtcgg cgggacgcaa
    11221 cgtgacgacg acctgctcct gctcctggcc ggagacgacg acatcgcgga ctcccgggag
    11281 agtcttgagc ttggcggcga cgatgtcttt gagcttcccg gagaggatcg ccttgctctc
    11341 ggtggacgac acggccagga cgatgatcgg cacgtcgtcg aaccggcccg cgacgacctg
    11401 cggcgtcacg tcgctcggca gccggccctg tatgccgtcc acggccgaac gcagatcggc
    11461 cagcaccttg tccgagccag tcccgaactc ccactcgacg cgcaccgtcg agacggcgct
    11521 cgcgctgcgg gaggtgactc gggtgatgcc cgagacggcc ttcgcggcgt cctcgatcgg
    11581 gcgactgacc tcacgctcga cgacctcggg agcggcaccc ggcagcaccg acacgatcgt
    11641 cgcggcgggt gtctcgatcg aggggatgag ctcctgcttc aggctgctgg tggcgaacag
    11701 ccccaggccg atgaggatcg cgcagacgag cagaaccacc gtgcggttgg ccaggctcaa
    11761 cttggtcagt cgggtcactt ggacagacta cgtgcgtttg cggcgggcct tgacaccggt
    11821 gtcggcaacc cgagcggcgc acccgcgccc gagtcagtcg accgggtcgg ggggaacctc
    11881 accgcgcatc aaggggcccg aggtgtggcc gcccaggtgc tccatcgcga ggcgcccgac
    11941 gagttgctgg ttggtcgtca cgcgctccga acgaccgttg ctcaggaagc tcaaggccca
    12001 ccgaacgagc gtcgacacct gctgcttgaa cccggcgatc atcagcaagt gcagcagcag
    12061 ccaggccagc caggccagga agccggtgag ccgcagcggc ccgaccctca ccacggcctc
    12121 gtacttggcg atcgtcgcca tgctgccctt gtccttgtag gcgaacgggc ccggtgcggg
    12181 ccggtcctcg acgcgggcgg cgatggacgc cgcggcatac tgcgccgatt ggatcgccgc
    12241 gggagccacg cctggcacgc cgggcaggct catcaggtcg ccgatgacgt agacctccgg
    12301 gtgccctggc agcgtgaggt gatcgttcac ctcgacccgg ccggcccggt cgacggtcgc
    12361 ccccgcctgg tccgcgacga gccgccccag cgggctgccc tccacacctg cggcccacac
    12421 cttgcatact gcctcgatgc gctcggtgct gccgtcggcg tgcctgagcg tcaacccggt
    12481 ggcgtcgagc gaggtgacca tggcgtgcag gaccacgtcg accccgagcc ggctgaggtc
    12541 ccgttcggcc tttgctccca actgctcgcc gaacgccgac agcacctgcc cggcgccgtc
    12601 gacgaggatc acccgagcct tggtggggtc gatccggcgg aactcgcggg cgagcgtgcg
    12661 attggcgagc tcccggatct gtccggccat ttcgacgccg gtcgggcccg cgccgacgac
    12721 gacaaaggtc agcagccggc tcacctcctc cgggtcgtcg gcgatctcgg cgagctcgaa
    12781 ggccgcgaag atccgggcgc gcagctcgag agcatcgtcg atcgacttca tgccgggggc
    12841 gaaggtggcg tactgcgggt tgccgaagta ggactggccg gcaccactgg cgatcaccaa
    12901 cgagtcgtag gcgctcgtcc gctcctcatc gtggaaccgc cagccgatcg tcttggactc
    12961 gaggtcgatg gtctcgacga gcccctgcac gacccggacg ttcttctggc ggcgcaggat
    13021 ctcgcgcgtc gagggggcga tctcgccctc agacaggatg ccggtcgcca cctgatagag
    13081 cagcggttgg aacaggtggt ggctcgtcct gtcgatgagg gtgatctcga cgtcggcctt
    13141 ccgtagcgcc ttcgtcacga acagaccccc gaaccccgaa ccgatcacga ccacacgatg
    13201 tgtcacctcg gcctcccttc gtcactgctc acaacaccac cgaggttgcc gcccttcccc
    13261 gttcggctga cctggttggg cccggtgggc ccggctggat ccgcggttag tcgcggctct
    13321 gccaggtgca gatgcaggcc tcgttgccct cggcgtcggc gaggacccag aacgccggag
    13381 cgcggacgtc gctgacgagg cgacctccgg cggcaagggc gcgggccagg cgaccggggg
    13441 cctcgtcatg gctgacggtc acatccaggt ggatccgatt gcgttgcggc cgaggcgaat
    13501 ccatctgctg gaaccacacg gcgggcccga cccggtcagg gtcgacaagc tcgtcgccac
    13561 cgttgtcggg ccgatcgagc accgcggccc agaacggtcg gatcgcgtcg cgatccatag
    13621 cgtcgatggc gatctcgacc tcagtcaggc ggcccggctg gctggtgagg tccatctcct
    13681 gcacgacggc atcgatggct ttcccgagac gaatgtcccg ctccgtgacg ccgccgacgt
    13741 cgtggcttcc ggtggcgatg tgcacgcgac cccaccgcag gtcgatctcg gggtggtgtc
    13801 cctgctcctc ggcgatggc
//
LOCUS       DAORPW010000053        13592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_920_length_13592_cov_756.152323, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000053 DAORPW010000000
VERSION     DAORPW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13592
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_920_length_13592_cov_756.152323"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="PLB86_06240"
     CDS             complement(<1..1355)
                     /locus_tag="PLB86_06240"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRC64770.1"
                     /translation="MNELETTMWRSERHPQQSSTICSLLILDTVPEWDRLVAAHEWAT
                     SLVPRSRQRVVEPIVPTGPPVWRTDPHFALTYHLRRMHVGPDGSMADLMELVQTLAMA
                     PFDRTRPLWEGTLIEGLDGGRAAYLLKLHHSLTDGLGAIQLMSLVQSRTREHTPDKPT
                     APEPDPYDVPASDDALSVTVEGARHTVGQLPGLALAAADAGISLARNPGGAVAETLRF
                     AASLRRVLSPPPAPPSPLLRNRDGRVWVMRTLECPLADLRAAAKRAGGSVNDAYLAAL
                     LGGLRRYHEHHGVEIDELPITVPVSLRRSDDPMGGNKFAGAMLAAPIGIVDPAERVAA
                     LRGVILASLAEPALDSFSVITPVVNRLPSAVGAAVMGLGAATDLSASNMPGLPYEVFM
                     AGARVEQVYPFGPLPGVAMMAAMMSHVGTCCIGMTIDGTAVADVDVLMRCMQDGLDEV
                     LA"
     gene            complement(1430..>1870)
                     /locus_tag="PLB86_06245"
     CDS             complement(1430..>1870)
                     /locus_tag="PLB86_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC64771.1"
                     /translation="FTVVAKKEARFDPRSLVGSLLLEPAYIDRSNSEQSRATLDALVS
                     RIRGGTSLLIFPEGTRSTTQVLGRFRKGGFHLAIQAGVPVVPIVLRNTGEILPRHGRV
                     LRPGNVDIAVLDPIDDWTVEDLDAQVTALRDRFAATLADWPGAR"
     assembly_gap    1871..1970
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1971..2675)
                     /locus_tag="PLB86_06250"
     CDS             complement(<1971..2675)
                     /locus_tag="PLB86_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HRC64772.1"
                     /translation="MDFAEQLARIETAPQGASVGAFFDLDGTLVSGYTASTFFTDRVK
                     SREVDLGHFMRTFVAVIDGTYLGGEPTRGAIKGYAAMRGQSEETMTELGERLFVQKIA
                     GTIRPEARELVRAHQRRGHTVVIASAASKYQIQPVATDLGIHHLVCTQLAVEDGILTG
                     ELDGRLLWGTQKASGVRSFARSHDIDLKASFAYGNGAEDVAFLGAVGSPIALNPHPGL
                     RGAAKTFGWPVVMLRDP"
     gene            complement(2662..>3051)
                     /locus_tag="PLB86_06255"
     CDS             complement(2662..>3051)
                     /locus_tag="PLB86_06255"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64773.1"
                     /translation="DGSDLYVANLVLRPFVDAYLVVADRLAAAGDSAVNEADVLDEAL
                     RVGQQWELERRIASAESVSLELYRTGLRLARHRGLLGGEGADTAYPGDSLGARRAAFL
                     VELQDVATQLDTIARITQASRSARGLR"
     assembly_gap    3052..3151
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3152..4631)
                     /locus_tag="PLB86_06260"
     CDS             complement(<3152..4631)
                     /locus_tag="PLB86_06260"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRC64774.1"
                     /translation="MTGSGPDIEALLADPGLAASLERYAAEHDLPIGPVRAEAAEHLR
                     EMVATHNPRATQSWDKLGAWIMRAHDVLVDEEDMARLKALDREHSLAIVFSHRSYLDG
                     WVLPNVMSSRRFSPLFTFGGANLDLPVVGTLVSRTGIIFIKRETKEMPVYRLTLRAYI
                     SHLVQRRANLAWSIEGGRTRTGKLRPPVHGILRYLSDAAEASDGPDVMLVPVSIVYDQ
                     LHEVAGMTAEARGSRKRPEDLGWLIRFARSQGGRLGRAYVSIGEPFPLRQRMATLRAE
                     GNDTSQAVERVAIDASHRINRATPVTTVAVVCLALLGADRALTFERVLDTVEPLARYI
                     RDRRWPVAGAANLTDRSTIRRALQELVASGVLTVFEAGTEPVWRIAPDQHLVAAFYRN
                     TVIHILVDRAIGEVALLDAIAAGEGADVERAAWERAKALRDLLKFEFFFPGRDDFERE
                     LRAELALMAPGGAGALTLDSARALLDGSDLYVANLVLRPFVDA"
     gene            complement(4775..5923)
                     /locus_tag="PLB86_06265"
     CDS             complement(4775..5923)
                     /locus_tag="PLB86_06265"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRC64775.1"
                     /translation="MAEAYIVSAVRTPVGRRGGGLAGVHPADLSAHVMRAAVETAGID
                     PAAVDDVVWGCVDQIGAQATNIARTGWLSAGLPESVPGTTIDRQCGSSQQAVSFAAYG
                     ILAGQQDLVLAGGVEAMSLVPIWSPATVGAAQGLGHPFGGQGWTARYGEAEVSQFYGA
                     ELIADRWDVTRDDMERFALASHQRALAAQAAGAFADEIVPYAAVAADEGPRADTSLEK
                     MATLKTVRDGGRVTAAVSSQVSDGAAALLLASADAVRTHGLTPLARIHALTVVGSDPI
                     VMLDGPIAATERVLARAGLTIGDIDQSEVNEAFAPVVLAWLKATGADPARTNPLGGAI
                     ALGHPLGATGARLMVTLVHALRRGGGRYGLQTMCEGIGMANATVIERC"
     gene            complement(6260..6628)
                     /locus_tag="PLB86_06270"
     CDS             complement(6260..6628)
                     /locus_tag="PLB86_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRC64776.1"
                     /translation="MDIQRFESNARMSQAVVYGGLVYLAGQVGAGETVAEQTTNVLAS
                     IDRLLAAAGSDRSRILTATIWLTDMATFGEMNGVWEQWVLPGSTPARATVEARLAAPA
                     YLVEISVTAAVAPCPLTTTL"
     gene            6756..7532
                     /locus_tag="PLB86_06275"
     CDS             6756..7532
                     /locus_tag="PLB86_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRC64777.1"
                     /translation="MTSDSHGPDVSDRQAHAEATFTRLFGPRDVNAPEDDPELMSILR
                     GFIFGDVFDTGVLDDRARELVTVTVLACLQTLPQLKAHTAAALHVGVEPIEIREAVYQ
                     LAPFIGFPRTLNAVATINEAFRAQGIELPLPDQGTVTDADRYARGFAEQGPLYGDEIK
                     ANLADLPEPFNEALPRFLTEFCFGDFYTRGGLSLAQRELLVLCALATIGDTSAQLGPH
                     ARACIQVGNSTAYVIAALVHCFPYIGFPRAVAAIRAVKSL"
     gene            7560..8234
                     /locus_tag="PLB86_06280"
     CDS             7560..8234
                     /locus_tag="PLB86_06280"
                     /inference="COORDINATES: protein motif:HMM:NF007788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HRC64778.1"
                     /translation="MNVAFLTIGQSPRHDMADPIEESLPAGTTVVHSGLLDGLSRPEI
                     EAAYGDGGAGQGLITRLADGSSVTMRVEAVEEGLRTKVASLTDGGVDAIVLLCTGEFP
                     SLRSGDVWLIEPDTVLTGYVVSLFAQVRVGMILPMAGQVDDARVKWRGLADPIFAFAS
                     PYSDDVEALVAAAYDLVDAGARALVLDCMGFSRRHREALRARGVEVPVLVANEVVGGV
                     VRIAFS"
     gene            complement(8281..9051)
                     /locus_tag="PLB86_06285"
     CDS             complement(8281..9051)
                     /locus_tag="PLB86_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRC64779.1"
                     /translation="MSTLANARDVLRLLASHPRDLTVTDVAAGLDAPKSSVSRTLGMM
                     AEFGFLERDPQTLAYRPGPLILEAASHVRGSTDVVALLNEALVRLVDTTGYTGYLNLL
                     EGGETLVIRMRQGRAGRLQAYTPEGTRGTAYASSSGRAILARLTDEDVSGLIGTRLEP
                     AGSAPRTLPELLERLAQVRAEGWSLSRGESVHGVAGVASAVVDPTSGRPYGIGIGLPV
                     QDLETTDPQLLGEAVRDAAHSVGRKVADPFWLGFPTTN"
     gene            9259..10947
                     /locus_tag="PLB86_06290"
     CDS             9259..10947
                     /locus_tag="PLB86_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HRC64780.1"
                     /translation="MAHDTSATTDQVTERHPRALAPANLILTVLLSVFGALIGIQIIA
                     TLGITPSTSLLGALVAMTLSRIPLTIFRGFRNVHAQNLAQTSISSATFGAANSLFLPI
                     GIPFLFGQPSMIMPMLIGVAAAMLLDAWLLYRMFDTPAFPARRPWPLGIAAAEAIKAG
                     DQGGAQAKVLFGGIGVGTLGAIFSLPMSAFGVAMIGGFGAMMAFGIGLLTRAYAKPVA
                     QIDLMALYIPHGMMIGAGLVALVQVGRVVVSAHRNGDKDTDEPESTSTGSKASASSAG
                     SSAASTAVATADPVNAASRLGQSLRVGGIGYLIIAALLAFGTGVFTQMSTGMLISFVL
                     YATVAAFVHELIVGIAAMHSGWFPAFAVALITLIIGIMIGFPPAALVVLAGFTAATGP
                     AFADMGYDLKAGYVLRGGGIDPAFELDGRRQQLIAAMVGFGVAIIAVAAAYPVFFGRN
                     MTAPIDAAYIAAIKAGVSGETARSLVIWAIPGAIIQFLGGSRRQLGVLFATGLLISTP
                     LAGWMVAGGLVVRIVASKVLGPVARERLEVFAGGVIAGDALYSFFSGAVKSLRS"
     gene            10989..12062
                     /locus_tag="PLB86_06295"
     CDS             10989..12062
                     /locus_tag="PLB86_06295"
                     /inference="COORDINATES: protein motif:HMM:NF017812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 family protein"
                     /protein_id="HRC64781.1"
                     /translation="MKRTLTPDDIEAAVYGGAILGGGGGGFIEPGLRTARLALQVGSP
                     QLWSVDEFDEDVLSATVALVGAPAAPNALVQPAHLIRALDLLQSQLPGGRVAAINTNE
                     NGAETTVNGWFHSALTGVPVIDLACNGRAHPSSVMGSMGLHLDPDYTSIQAYAGGHPE
                     FYVEGVTKGRMEATSSVVRRASVEADGWVGVARNPVSVGYAASNGAAGAISFAIELGR
                     VFLDGGVQAVTERLGGRIIASGTVGSYSCEPVEGLDIGKVVLTDAAATTLLFVNEYMV
                     AEHDGERVGAFPDLIMTFDENGVPLPSARVQPGQTISVLVAPASSLILASTMFMADLY
                     EPLEKALGVQFAPRSGVLAGATA"
     gene            12059..13543
                     /locus_tag="PLB86_06300"
     CDS             12059..13543
                     /locus_tag="PLB86_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRC64782.1"
                     /translation="MTDTHSHPSPSGIPLGGGCDPALALGEVQRRWRSDLLDQMGDFI
                     RIPALSPAFDADWVEHGFLDQMVELAAAWLTAQRLPGAVIEILRSPGRTPLLFVDIPA
                     QGTAAEATVLVYGHLDKQPEFQGWREGTGPWTPVFDGIRLFGRGSADDAYAVCLLGAA
                     LGALVAQGASYPRCVAVFETGEESGSPDLAYWLGVLGDRLGQVGLVACLDSGAGDYDR
                     LWAVTSLRGTASATLTVRVLDEGAHSGDAGGVVPSSFRVLRALLDRVESSATGEILLP
                     ELRCDIPADREDQARATAELLGASLWERFSWASSADGRVRACTEDPHEALLRRAWHPS
                     LAVVGAEGMPPLATAGNVLRPFTSIRLSLRLPPLCGAAAALAAVGEVLTADPPYGAVV
                     TFEPSPQVVDGWNAPPFSVELSAALNAASAACFDGAAPAFIGQGGTIPLMGLLGGLFP
                     DAQFLACGVQGPGTNAHGPNESLHIPYTERLTASLAVVINALAL"
BASE COUNT         1990 a         4635 c         4682 g         2085 t
ORIGIN      
        1 gcgagaacct cgtcgagacc atcttgcatg caccgcatga gcacatcgac atcggccacg
       61 gcggttccgt cgatcgtcat cccgatacag caggtgccga cgtgcgacat catcgccgcc
      121 atcatcgcga cgcccggcag cgggccgaac gggtagacct gctccacgcg ggcaccggcc
      181 atgaagacct catagggcag cccgggcatg ttcgacgccg acaggtcggt ggccgccccg
      241 agccccatga ccgcagcgcc gaccgccgac ggcagccggt tgaccaccgg ggtgatgacc
      301 gagaacgagt cgagagcggg ctccgcgagc gaggcaagga tcaccccacg cagcgccgca
      361 acccgctcgg ccgggtcgac gatgccgatc ggcgccgcga gcatggctcc ggcgaacttg
      421 ttgccgccca tcgggtcatc gctgcgtcgc agggacacgg ggacggtgat gggcaactcg
      481 tcgatctcga ccccgtggtg ctcgtgatag cggcgcaggc cgccgagcag ggccgcaagg
      541 taggcgtcgt tgaccgaccc cccagcccgt ttggccgcgg cccgcaggtc tgccagggga
      601 cactcgaggg tgcgcatgac ccacacccga ccgtcgcgat tgcgcagcag cggcgacggc
      661 ggggccggcg gtggcgacag cacccggcgc aacgacgcgg cgaaccgcag agtctcggcc
      721 accgcgccac cagggttgcg cgccaacgag attcccgcat cagccgcggc gagggccaag
      781 cccggcaact gcccgacggt atgccgcgcg ccctccacag tcaccgacag cgcatcgtcg
      841 ctggccggca cgtcataggg gtcgggctcg ggtgccgtcg gcttgtccgg cgtgtgctcg
      901 cgggtgcggc tctggacgag cgacatgagt tggatggcgc cgaggccgtc ggtgagtgag
      961 tggtggagtt tgagcaggta tgccgcgcgc ccaccgtcca gcccctcgat gagcgtccct
     1021 tcccagaggg gtcgggtgcg atcgaacggg gccatcgcca gggtctgcac gagctccatg
     1081 aggtcggcca tcgacccgtc cgggccgaca tgcatccgac gcaggtggta ggtcagcgcg
     1141 aagtgcgggt cggtccgcca cacgggcggg ccggtgggaa cgatcggctc cacgacccgt
     1201 tgccgcgaac gcggcaccag cgacgtggcc cactcgtgag cggcgaccag ccgatcccac
     1261 tcgggcacgg tgtccaggat gagcaggctg cagatcgtcg aggactgttg cgggtgccgc
     1321 tcggagcgcc acatcgtcgt ctcgagctcg ttcatgcggt cgtcctgcgc ccaatcccgt
     1381 tccgggccag gctcgtccgg aaaggccccg ggtccgcgcg gggtggcgtt catcgggcac
     1441 ccggccagtc ggccagcgtc gcggcgaagc ggtcacgcaa ggcagtcacc tgggcgtcga
     1501 ggtcctcgac agtccagtcg tcgatcggat cgagaaccgc gatatcgacg ttgccaggcc
     1561 gaagcacccg gccatgccgc ggcaggatct cgccggtgtt gcgcaggacg atcggcacga
     1621 caggcacccc cgcttggatc gccagatgga aaccgccctt gcggaaacgg ccgagcacct
     1681 gcgtcgtcga gcgggtgccc tcggggaaga tgagcagcga cgtcccgccc cggatccggg
     1741 agacaagagc atcgagcgtc gcgcgggact gctcggagtt gctccggtcg atgtatgccg
     1801 gttccaacag caggctgccg accagactcc gcgggtcgaa ccgcgcttcc ttcttggcca
     1861 cgacggtgaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgggtcgcgc
     1981 agcatgacga ccggccagcc gaaggtcttg gccgctccgc gcagccccgg gtgcggattg
     2041 agcgcgatcg gcgacccgac agccccgagg aacgcgacgt cctcggcacc gttgccgtag
     2101 gcgaagcttg ccttgagatc gatgtcatgg gatcgggcga aagaccgcac cccactggcc
     2161 ttctgggtgc cccacaacag gcgcccgtcc aactcgccgg tgaggatgcc gtcctcgacg
     2221 gccaactggg tgcagacgag gtggtggatg cccaaatcgg ttgccaccgg ctggatctgg
     2281 tacttactcg ccgccgaggc gatgacgacc gtgtggcccc gacgctggtg ggcgcgcacg
     2341 agttcgcgtg cctcggggcg gatcgtcccc gcgatcttct ggacgaaaag tcgctcgccc
     2401 aactcggtca tcgtctcttc gctctggccg cgcatcgcgg catacccctt gatggcccct
     2461 cgcgtcggct ccccgccgag gtacgtgccg tcgatgaccg cgacgaaggt gcgcatgaag
     2521 tgccccaggt cgacctcgcg gctcttcacc cgatcggtga agaaggtgct cgcggtgtag
     2581 cccgagacga gggtgccgtc gaggtcgaag aaggcgccga cactcgcccc ctgcggtgcg
     2641 gtctcgatcc gcgcgagctg ctcagcgaag tccacgggcg ctcctgctgg cctgagtgat
     2701 gcgggcgata gtgtccaact gcgttgccac gtcttggagt tcgacgagga aggccgcccg
     2761 ccgcgccccc agcgaatcgc ccgggtatgc cgtgtcggca ccttcgccac ccagcagacc
     2821 gcggtggcgg gccaggcgca gaccggtgcg atacagctcg agcgacaccg actcggcgct
     2881 ggcgatccgg cgttcgagct cccactgctg gccgacacgg agggcctcgt cgagcacatc
     2941 cgcctcgttg accgctgaat ccccagctgc cgcaaggcga tcggcgacca cgagataagc
     3001 gtcgacgaag ggcctcagca cgagattggc gacatacagg tcgctgccgt cnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagcgtcga cgaagggcct cagcacgaga
     3181 ttggcgacat acaggtcgct gccgtcaaga agcgcccgag ccgagtcgag ggtcagcgcc
     3241 ccggccccgc cgggagccat gagggccagc tcagcgcgca actcacgctc gaagtcgtcg
     3301 cggccgggga agaagaactc gaacttgagc aggtcacgca gtgccttggc ccgttcccag
     3361 gcggcgcgtt cgacgtcggc gccctcgcct gcagcgatgg cgtccaggag cgcgacctcg
     3421 ccgatggccc ggtcgacgag gatgtggatg acggtgttgc gatagaacgc ggcgacgaga
     3481 tgctggtcgg gggcgatccg ccagaccggc tcggtgccgg cctcgaagac cgtgagcacc
     3541 cctgaggcga cgagctcctg cagggcccga cggatcgtcg agcggtcggt gaggttggcc
     3601 gcacccgcga ctggccagcg gcggtcgcgg atatagcgcg ccagcggctc aacggtgtcg
     3661 agcacccgct cgaaggtgag cgcccggtcg gccccgagga gggccaggca gaccaccgcc
     3721 accgtcgtca cgggcgtcgc ccggttgatc cggtggctgg cgtcgatggc gacccgttcg
     3781 acggcctgcg acgtgtcgtt gccttcggct cgcaacgtcg ccatccgctg acgcagcggg
     3841 aagggctcac cgatggagac ataggcccgg ccgagtcggc caccctgcga gcgggcgaag
     3901 cggatcagcc aaccgaggtc ctcggggcgc ttgcggctgc cgcgcgcctc cgcggtcatc
     3961 ccggcgacct cgtggagttg gtcgtagacg atcgacacgg ggacgagcat gacgtcgggg
     4021 ccatcactcg cctccgcggc gtcggagaga taacgcagga tgccatgcac gggtgggcgg
     4081 agtttgccgg tgcgggtgcg acctccctcg atcgaccagg ccaggttggc ccggcgctgc
     4141 acgaggtggg agatgtaggc gcgcagggtg agccggtaga ccggcatctc cttggtctcc
     4201 cgcttgatga agatgatccc cgtgcggctg accagagtgc cgacgaccgg cagatcgagg
     4261 ttggcgccgc cgaaggtgaa caacggcgag aaccggcgcg aggacatgac gttgggcagg
     4321 acccagccgt cgaggtagga gcggtgcgag aacacgatcg ccaggctgtg ctcccgatcg
     4381 agggccttga gccgcgccat gtcctcctcg tcgacgagga catcgtgagc ccgcatgatc
     4441 caggcaccga gtttgtccca ggactgggtg gcgcgggggt tgtgggtcgc gaccatctcg
     4501 cgcaggtgct cggcagcctc ggcgcggacc gggccaatgg gcaggtcatg ctcggcggca
     4561 tacctctcca gcgaggcggc cagccccggg tcggcgagca acgcctcgat gtcgggtccg
     4621 gagcccgtca ccgccaccac cgcctcgcga gcgcgtcgcg tcgctgaacc gacccatcct
     4681 cgggccgttt cggccagtcg ctgcgcatcc acgaggacag gtctaacgcc cggttggcct
     4741 cgggtcaacc gctatccgtc tccggaatca ccgctcagca gcgctcgatc acggtcgcgt
     4801 tggccatccc gatgccttcg cacatcgttt gcaggccata gcggccgccg ccgcgacgaa
     4861 gggcgtgcac cagggtgacc atgagcctcg cgccggtcgc tccgagcggg tggccgagcg
     4921 cgatcgcgcc tccaagcggg ttggtgcgag ccgggtcggc gccggtcgcc ttcagccagg
     4981 cgaggacgac gggcgcgaag gcctcgttga cctccgactg gtcgatgtct ccgatggtga
     5041 ggccggcgcg agccaggacc cgctcggtcg cggcaatcgg gccgtcgagc atgacgatcg
     5101 ggtcggaccc gacgaccgtg agcgcgtgaa tgcgcgccag cggggtgagc ccgtgggtgc
     5161 ggaccgcgtc tgcggaggcg agcaggaggg cggcggcgcc gtcggagacc tggctggaca
     5221 cggccgcggt gactcggccg ccgtcgcgga cggtcttgag agtcgccatc ttctccagcg
     5281 acgtgtcggc gcgcggcccc tcatcggcgg cgacggcggc atacgggacg atctcgtcgg
     5341 cgaacgcacc ggcggcctgc gcggcgagcg cgcgctggtg actggcgagc gcgaaccgct
     5401 ccatgtcgtc acgcgtgacg tcccagcggt cggcgatgag ttcggcgccg tagaactggc
     5461 tcacctcggc ctcgccatag cgcgccgtcc acccctggcc accgaacggg tgcccgagtc
     5521 cctgcgcggc gccgaccgtc gcggggctcc agatcgggac gaggctcatc gcctcaaccc
     5581 cgcccgcgag caccagatcc tgttgtcccg cgaggattcc gtatgccgcg aagctcaccg
     5641 cctgctgcga cgagccgcac tggcggtcga tggtcgtgcc gggcaccgac tcgggcagcc
     5701 ctgcgctgag ccagccggtg cgggcgatgt tggtggcctg agcgccgatc tgatcaacgc
     5761 acccccagac gacgtcgtcg actgcggccg ggtcgatacc tgcggtctcg acggcggccc
     5821 gcatgacgtg ggccgagaga tcggccgggt ggactccggc caggccccct cctcgacggc
     5881 ccacgggggt gcgaacggcg ctgacgatgt atgcctcggc catgactcca ccaaagcacg
     5941 ttccgtggcc ggcggggagg tgaggtgggg gcgcaggtgc ccgccatgac cgatctcacg
     6001 tctcgggccc cgaatggcca cgagcgcggg gcggcggagg tgaggacggg cggcgcgcgg
     6061 catacccggg ggtggggcag cccatgaagc cgcgccaggt ggggtgatca cgcaccctgc
     6121 gactgcttca tgcgcggcct cacccccgcg cgccgtggcg gcgcccatga cggcgtccct
     6181 actgctgctg caacagcagc caccgcgccg tcatcgacgc cgtcatgtgt cgcgcgggca
     6241 ggaggttggc aaccgggctt cacaaagtgg tggtgagtgg gcagggcgcg acggccgctg
     6301 tcaccgagat ctccacgagg tatgccggtg ccgcgagccg cgcttccacg gtggcccgcg
     6361 cgggagtgga accgggcagg acccactgct cccaaactcc gttcatctcg ccgaaggtcg
     6421 ccatatcggt cagccagatc gtggcggtga ggatccgcga ccggtcgctg ccagccgccg
     6481 cgaggagccg atcgatcgac gcgagaacgt tcgtcgtttg ctccgccacg gtctcccccg
     6541 caccgacctg cccggcgagg tagacgaggc cgccgtagac cacggcctgg ctcatgcgtg
     6601 cgttcgactc gaagcgttgg atgtccatgg gcagatcctc ccagcgcggg acacttcctt
     6661 ccaggcgcgg gacgtcgcgg gcaccgcgcg aacgacctct ctctcgcagc agggcctgac
     6721 ttgaccgggg cggggcgcgg gacaaaggtg ctcgcatgac ttccgactcc cacggtcccg
     6781 acgtgtctga ccgtcaggcg catgccgagg cgacgttcac ccggctgttc gggccgcgcg
     6841 acgtcaatgc ccccgaggac gaccccgagc tgatgagcat cctgcgcggc ttcatctttg
     6901 gggacgtctt cgacacgggc gtcctcgacg accgcgcccg cgagctcgtc accgtgacgg
     6961 tcctggcctg cctgcagacc ctgccccagc tcaaggcgca cacggctgca gccctgcacg
     7021 tcggcgtcga gcccatcgag atccgcgaag ccgtctatca gctggcgccg ttcatcggct
     7081 tcccgcgcac cctcaacgcc gtggcgacga tcaacgaggc cttccgggct cagggcatcg
     7141 agttgccgct gcccgaccag ggcacggtca ccgacgcgga ccgttacgcg cggggatttg
     7201 ccgagcaggg gcccctctac ggagacgaga tcaaggccaa cctggcggac ctccccgagc
     7261 cgttcaacga ggccctgccc cggttcctca ccgagttctg cttcggcgac ttctacacgc
     7321 ggggcgggct gagcctggcc cagcgcgagt tgctggtcct gtgcgccctg gcaacgatcg
     7381 gtgacacctc cgcccaactc ggcccgcacg cgagggcgtg catccaggtc ggcaacagca
     7441 cggcatacgt gatcgcggcc ctggtccact gcttccccta catcggcttc ccccgcgccg
     7501 tcgccgcgat ccgcgccgtc aagagcctct agccagccgt cgcaaaggac accgccctca
     7561 tgaacgttgc gttcctgacc atcggccagt cgcctcggca tgacatggcc gacccgatcg
     7621 aggagtcgct tcctgcgggc acgacggtcg tgcacagcgg gctgctcgac ggtctgagtc
     7681 gacccgagat cgaggcggcc tatggcgacg gcggcgcggg gcaggggctc atcacccgac
     7741 tggccgacgg cagcagcgtg acgatgcggg tcgaggcggt ggaggaaggc ctccgaacca
     7801 aggtcgcctc gctcacggat ggcggcgtcg acgcgatcgt cctgctgtgc accggcgaat
     7861 ttccgtcgct ccgttccggc gatgtgtggc tgatcgagcc agacacggtg ttgaccggct
     7921 atgtcgtcag tctgtttgcc caggtccggg tcgggatgat cctcccgatg gccggccagg
     7981 tcgacgacgc gcgagtgaaa tggcgcgggc tcgccgaccc catcttcgcc tttgcgtcgc
     8041 cctactccga cgacgtcgag gcgctggtcg ccgcggcata cgatctcgtc gatgctggcg
     8101 cccgcgcgct ggtcctggac tgcatgggct tcagccgacg gcatcgggag gcgctccggg
     8161 ctcgcggggt ggaggtgccc gtgctggtgg cgaacgaggt cgtcggcgga gtcgtgcgaa
     8221 tcgccttctc ctgaaagcct ggacggcgtc gaaagagtgg cgccgagcgc caggcaccgc
     8281 tcagtttgtg gtggggaagc ccagccagaa cggatcggcg accttgcgtc cgaccgagtg
     8341 cgcggcgtcg cgaacagcct cgccgagcag ctgcgggtcg gtcgtctcga ggtcctggac
     8401 cggaagaccg atcccgatgc cgtagggccg cccggaggtg gggtcgacga cggccgaggc
     8461 cacaccggcc acgccatgca ccgactcgcc gcgcgacagc gaccacccct ccgcgcggac
     8521 ctgggcgagt cgctcgagca actccggaag ggttcgcggc gcggagccgg ccggctccag
     8581 gcgcgttccg atcagccccg acacgtcctc gtcggtcagt cgcgccagga tcgcgcgacc
     8641 actggacgag gcgtatgccg tcccccgcgt cccctcgggc gtgtaggcct gcagccttcc
     8701 ggcccgcccc tgacgcatcc ggatgacgag ggtctcaccg ccttccaaga ggttgagata
     8761 ccccgtgtaa cccgtggtgt cgacgaggcg gaccagcgcc tcattgagca gggccacgac
     8821 atcagtagag ccgcggacat gtgacgcggc ttcgaggatc agggggccag gacggtaggc
     8881 cagcgtctgc ggatcccgtt ccaggaagcc gaattcggcc atcatgccga gggttcgtga
     8941 cacggaactc ttgggtgcgt cgagaccggc ggcgacgtcg gtcaccgtga ggtcgcgggg
     9001 atggctggcc agcaggcgga gcacgtcgcg tgcgttcgcc agcgtgctca tctgcccctc
     9061 cttcgcggtc ttgtcgatcg tagtgggcgt gacggacggc cacagcgcca cacgggccgc
     9121 tgcctccgac ccttgacaag cgtcaccacg accatcacca tgtccatatt ctcgaacccg
     9181 gtcctaaata gtagaacgtg actggggtac ggcaggaaga gtcgcctcaa ggagaacccc
     9241 cggacaagga gtacgtacat ggcccacgac acgtcggcga cgacggacca agtgacagag
     9301 cgtcatcccc gggccctggc gcccgcgaac ctgatcctca ccgtcctgct cagcgtgttc
     9361 ggggcgctga tcggcatcca gatcatcgcc accctaggaa tcacccccag cacgtccctc
     9421 cttggcgcgc tggtggccat gaccctctcg aggatcccgc tcacgatctt ccgcggcttc
     9481 cggaacgtgc atgcccagaa cctcgcccag accagcatca gttcagcgac gttcggtgcg
     9541 gccaacagcc tcttcctgcc gatcggcatc ccgttcctct tcgggcagcc gagcatgatc
     9601 atgccgatgc tcatcggcgt cgccgcggcg atgttgctgg atgcctggct cctctaccgg
     9661 atgttcgaca ccccggcctt cccggcccgt cggccgtggc ccttgggcat cgctgccgcg
     9721 gaggccatca aggccggcga ccagggcggg gcgcaggcga aggtcctctt cggcggcatc
     9781 ggcgtcggca ccctcggagc aatcttctcg ttgcccatgt cggcgttcgg cgtcgccatg
     9841 atcggcgggt tcggggcgat gatggccttt gggatcggcc tgctcacgcg tgcctacgcc
     9901 aagccggtcg cccagatcga cctcatggcg ctctacatcc cccacgggat gatgatcggc
     9961 gccggtctcg tggcgctggt gcaggtgggc cgggtcgtcg tctcagcaca ccgcaacggc
    10021 gacaaggaca cggacgagcc ggagtccacg tccaccgggt caaaggcctc ggcatcgtct
    10081 gcgggatcgt cggcagcgtc gaccgcggtc gcaaccgccg acccggtcaa cgccgccagc
    10141 aggctcggac agtcgctgcg tgtcggcggc atcggttacc tcatcatcgc cgcgctcctt
    10201 gctttcggga ccggcgtctt cacccagatg agcacgggga tgctcatcag cttcgtcctc
    10261 tacgccaccg tcgcggcatt cgtccacgag ttgatcgtcg gcatcgcggc gatgcactcg
    10321 ggctggttcc ccgccttcgc ggtcgctctc atcaccttga tcatcggcat catgatcggc
    10381 ttcccgcccg ccgccctcgt ggtgctggcc ggcttcaccg cggccaccgg cccggccttc
    10441 gccgacatgg gctacgacct caaggctggc tacgtcctgc gcggcggcgg catcgacccc
    10501 gcgttcgagc tcgatgggcg tcgtcagcag ttgatcgctg cgatggtcgg gttcggcgtc
    10561 gcgatcatcg ccgtcgcggc ggcatacccg gtgttcttcg gccgcaacat gaccgcgccg
    10621 atcgacgcgg catacatcgc ggcgatcaag gccggagtct ccggtgagac ggcgcggagc
    10681 ctggtgatct gggcgatccc cggcgcgatc atccagttcc ttggcgggtc gcgtcgccaa
    10741 ctcggcgtgc tgttcgccac cggcctgctg atctccaccc cgctcgccgg ctggatggtc
    10801 gcgggcggtc tcgtggtgcg cattgtcgcg agcaaggtgc tggggcccgt cgccagggag
    10861 cggctcgaag tcttcgccgg tggcgtcatc gccggagacg ccctctactc cttcttctcc
    10921 ggggccgtga agtccctgcg ctcctaacgc ttgatccccc aacactcacc cagacccagg
    10981 aggctcccat gaagcgcacc ctcactcccg acgacatcga agccgccgtc tacggcgggg
    11041 cgatcctggg cggaggtggc ggcggcttca tcgagccggg cctgcgcacc gcccggctcg
    11101 ccctgcaggt cggcagcccc cagttgtgga gcgtggatga gttcgacgag gacgtgctca
    11161 gcgccaccgt cgcgctggtc ggcgcgcccg ctgccccgaa cgccctcgtc caacccgctc
    11221 acctcatccg ggcgctggac ctgctccagt cccagctgcc cgggggccgg gtggccgcca
    11281 tcaacacgaa cgagaacggc gccgagacaa cggtcaacgg ctggttccac tccgctctca
    11341 ccggggtgcc ggtgatcgac ctcgcgtgca acggccgcgc ccatccgtcg agcgtgatgg
    11401 gctcgatggg gctgcacctc gaccctgact acacctcgat ccaggcctat gccggagggc
    11461 acccggagtt ctatgtcgag ggtgtcacca aggggcggat ggaggccacg tcgagcgtcg
    11521 tgcgtcgggc ctcggtcgaa gccgacggat gggttggcgt cgctcgcaac ccggtgagcg
    11581 tcgggtatgc cgccagcaac ggggcggcgg gggcgatctc cttcgccatc gagctgggtc
    11641 gagtgttcct cgacggcggc gttcaggccg tcacggaacg gctcggcggc cgcatcatcg
    11701 cgtcgggcac cgtcgggtcc tactcctgtg agccggtgga gggcctcgac atcggcaagg
    11761 tggtcctcac ggacgcggcc gccacgacct tgttgttcgt caacgagtac atggtcgccg
    11821 agcacgacgg cgagcgcgtc ggcgccttcc cggatctgat catgaccttc gatgagaacg
    11881 gggtccccct gccgtccgcg agggtgcagc cggggcagac gatcagcgtc ctcgtggcgc
    11941 cggcgtccag cctcatcctg gcctcgacga tgttcatggc ggacctctac gagccgcttg
    12001 agaaggcgtt gggggtgcag ttcgcccccc gcagtggcgt gttggctggg gcaaccgcgt
    12061 gacggacacg cactcgcacc cgtccccgag cggcataccc ctcgggggcg ggtgcgaccc
    12121 agcgctggcg ttgggagagg ttcaacggcg gtggcgctcc gacctgctgg accagatggg
    12181 cgacttcatc cggatcccgg cgttgagccc ggcgttcgat gccgactggg tcgagcatgg
    12241 gttcctggac cagatggtgg agctcgccgc agcctggttg acggcgcagc gcctcccggg
    12301 tgccgtgatc gagatcttgc gctcccctgg gcgcacgccg ctgctgttcg tcgacatccc
    12361 cgcgcagggg accgccgccg aggcgaccgt gttggtctac ggccacctcg acaaacaacc
    12421 ggagttccag ggctggcggg aggggaccgg cccgtggact ccggtgttcg acggcatacg
    12481 cctcttcggg cgtggctcgg cggatgacgc gtatgccgtg tgcctcctcg gcgccgcgct
    12541 cggcgccctc gtggcgcagg gcgcaagcta cccccggtgc gtcgccgtct tcgagaccgg
    12601 cgaggagtcc gggagccctg atctggccta ttggttgggc gtcttgggag accgactcgg
    12661 gcaggtcggc ctggtggcgt gcctcgactc gggagccggc gactatgacc gcctctgggc
    12721 ggtgacctcg ctgcgcggga ctgccagcgc cacgctgacg gtccgggtgc tggacgaggg
    12781 cgctcactcc ggcgatgccg ggggcgtcgt gccgtcgtcg ttccgggtgc tgcgcgccct
    12841 gcttgatcgg gtggagtcca gcgcgactgg ggagatcctg ttgcccgagt tgcgctgtga
    12901 catccctgct gaccgcgagg atcaggcgcg cgccacggcc gagttgctgg gcgcctctct
    12961 ctgggagcgg ttctcctggg ccagctccgc ggacggcagg gtgcgggcgt gcacggagga
    13021 tccccacgag gcgttgctgc gtcgcgcgtg gcacccgagc ctcgcggtgg tgggtgctga
    13081 gggtatgccg ccgctggcaa cggccggcaa cgtgcttcga ccgttcacgt cgatccggct
    13141 gtctttgcgc ctcccgccgt tgtgtggtgc ggcggctgct ctggcggcgg tcggggaggt
    13201 tctgacggcg gacccgcctt acggtgccgt cgtgaccttc gagccgagcc cgcaggttgt
    13261 cgatgggtgg aacgcaccgc ccttctcggt cgagttgtcg gctgcgttga acgctgccag
    13321 cgccgcgtgt ttcgacgggg ccgctcccgc gttcatcggg cagggcggca cgatcccgtt
    13381 gatggggctg ttgggcggcc tgttccccga cgcccagttc ctggcgtgcg gcgtgcaggg
    13441 gccggggacc aacgcccacg gaccgaacga atcactccac attccctaca ccgagcgact
    13501 gaccgcgtcc ttggccgtgg tgatcaacgc gctggctctc tgacgtcgag gtggttggcg
    13561 cggccgcgca ccgtggcggc gcccatgacg gc
//
LOCUS       DAORPW010000054        13543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_929_length_13543_cov_691.682533, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000054 DAORPW010000000
VERSION     DAORPW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13543
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_929_length_13543_cov_691.682533"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..1264)
                     /locus_tag="PLB86_06305"
     CDS             complement(302..1264)
                     /locus_tag="PLB86_06305"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HRC64783.1"
                     /translation="MTSTSTEPAARRTPIDLRLDRPFRRRDALLAGLTDEDLRGPRFR
                     RLLHGIYISAAVEITPRIRVLAAMRTTTHPCFASHHTAATLLGATVPDSAWTHLGTTE
                     GAMSVRRQVKLRRYAVPPALAPQSPLPCTDAAQTFLDLARCLELVDLVVLGDGLVRQG
                     HITAPALLKAARQHEGPGAALARLAAGYVRPGVDSPMETRVRMLFVLAGFPEPVVNVE
                     VFDDAGRLRYRIDLAWPSAKVAVEYDGRHHINRQDQWTYDIRRREELEAEGWRFLVLT
                     APDIYRTPGTTLDRAESLLAAGGLRVRRRPDWFAHFPSQPSLSA"
     gene            complement(1532..3679)
                     /gene="glgX"
                     /locus_tag="PLB86_06310"
     CDS             complement(1532..3679)
                     /gene="glgX"
                     /locus_tag="PLB86_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRC64784.1"
                     /translation="MLHRSLTSRDHPPPPGVTLVEGGAQVCVYAGHADGVQVCLFEPG
                     DVHGETERRVPLTERVYGYWFGFVPGMGVGQRYGFRVSGPWAPHDGMRYNPAKLLLDP
                     YARAIEGEVTWVPEVYGHRTSSDLRPEDLYAIDERDSAAYVPRSVVVDGGFDWGDDRP
                     LRRPLTESVIYETHVRNLTMRLPGVPANLRGTYAGLAHPATIDYLTRLGITAVELLPV
                     HTFTTEPEVWRRGLINHWGYNTLGFFAPHARYAAARDPQGALDEFKGMVKLLHAAGIE
                     VILDVVYNHTAEQSARGATLSWRGLDNRAYYRLDGRGDDIDVTGCGNTVDVRHVVAVR
                     MILDSLRYWVQECHVDGFRFDLAVALGRGKNDEFDHDHPLLIALRTDPVLSQVKNIAE
                     PWDLGIQGWRTGQFPPLFSEWNDRFRDRVRSFWLTDLARNLAGQPGHGVQDLATRLAG
                     SADLFHHRDRGTIASINFVAAHDGFTTADLTAYNHKHNEANREDNRDGADNNSSWNHG
                     IEGQIDDGVSMLRHRSIRNLLATLLVSTGVPMLNGGDEFGRSQHGNNNAFCQDNDTSW
                     YDWDLAPWQADLLATTQFLIQLRAQHPVLRQRDFFSGGPVHLDGSKDLEWYGADGHPM
                     TMDRWGDARVRTLQMLLDGAHHGHQSLLVVFHGDAHEGTVTLPRPPGLTAYELLWDSA
                     WDRPPAQAAWVSPDEPAVLLPASMRIYRAVDPT"
     gene            3714..4190
                     /locus_tag="PLB86_06315"
     CDS             3714..4190
                     /locus_tag="PLB86_06315"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRC64785.1"
                     /translation="MTDPTALPNFPPPSDEHPLRGRVLDALQDLGIEPVIDSDGDVAF
                     QVGEPSQQLFVRCQDGDFPVMRIFGQWQIGAPAPEDVLARLMRCNEVNLEWQVAKCGV
                     AGDNLIVTCDQVVMPGADLGQLMQLSVTLVLQVVGAWYGSWDQGRDEAPGAGGSDG"
     gene            4183..4962
                     /locus_tag="PLB86_06320"
     CDS             4183..4962
                     /locus_tag="PLB86_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC64786.1"
                     /translation="MGEFVRLEVADGIGTIRLDRPKMNALNLQVQTEIEECAREATLR
                     DDVAAVIVYGGERVFAAGADIKEMQSMSYTDMAVAVEGLHSFTTTLARMPKPSVAAIT
                     GYALGGGCELAMCCDFRLAGESAQLGQPEILLGIIPGAGGTQRLTRLVGPAKAKDLIY
                     SGRFIKADEALAIGLVDRVVPDDQVYAAAREWMSQFVGGPRFALKAAKEAIDRGGELD
                     LDSGLALEKSLFSALFATHDRFVGMESFVVNGPGKAQFEGR"
     gene            4959..5294
                     /locus_tag="PLB86_06325"
     CDS             4959..5294
                     /locus_tag="PLB86_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64787.1"
                     /translation="MRRRADGLDVLVVYVPRASTEALLAALFAAGAGAVGDYRECAWV
                     TSGLGRFRPLPGANPAVGDVGELVTIEEDRCEVVIPTGRRDEVIAALRAAHPYEEPAF
                     HVIETAPLD"
     gene            complement(5297..6142)
                     /locus_tag="PLB86_06330"
     CDS             complement(5297..6142)
                     /locus_tag="PLB86_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRC64788.1"
                     /translation="MSATIRIVHRTGYSYAGGVTASFNEIRMLPRSTHEQQVVHSRID
                     ISPVPWTYTYTDYWGTTVTAFEVFERHESLNVVATSTVDVQRHTSEEPRLQWGDLTSL
                     DVTDTHCEMLEIGDRVAPPPDLLAAVAELRRTSATPWELAVAVVDLVHAEVKYVFGAT
                     GVHTRASEAWEQRSGVCQDMAHLVLGALRSVGIPARYVSGYLMPSRSGELGVPYAGES
                     HAWIQFWDDGWVGADPTNNIRPGDLHVEVAVGRDYRDVAPLTGIFTGSRTSDMFVSVE
                     MTRLS"
     assembly_gap    6191..6290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6294..7217)
                     /locus_tag="PLB86_06335"
     CDS             complement(6294..7217)
                     /locus_tag="PLB86_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRC64789.1"
                     /translation="MLSRIAESLYWIGRYLERAEDTSRLLDVQLHLSVEDPVIDARAS
                     ADRLLTVMGIDHEGPASEDLVLRRLCHDPDSPASIVSALKGARESARRSRETVSTEMW
                     EAINTTWYAVQRGELAKVRPALAFRWVRERCAVITATADGTMSHDQGWYFLVLGRSLE
                     RIDMTARLAASAATGPAATAWSSTLRACGAHHAFVRGHHDQDSDSEAAEFLLLDRLFP
                     RSIVHTLDTAMDALDRLTPPERRGLTEDEVARTLGRARAELEYRTRGEVLLDLPERMA
                     ELQITCARANAAISARYFEGAVAAEWRGGAQ"
     gene            complement(7211..8818)
                     /locus_tag="PLB86_06340"
     CDS             complement(7211..8818)
                     /locus_tag="PLB86_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRC64790.1"
                     /translation="MDTLFAGYAARERTFDEMFEAAPNEVRSGYTDVHNRLTRMSPAD
                     VRGRADFLARIYVEQGVTFDIGGEERPFPLDIVPRLLDADTWATVEVGLTQRVRALER
                     FLDDVYGPGDLFNDEVMPRTVVTSSPHFHRVVAGLTPANGVRVHVSGIDLVRDGDGRF
                     RVLEDNVRIPSGVSYVMTNRRALTSALPEVFADHRIRPVRSYPARLLAALRASAPSGV
                     SDPTVVVLTPGVYNSAYFEHALLARMMGCRLVEGRDLVCQAGRVMVRTTTGLRPVHVI
                     YRRVDDEFLDPVHFRGDSLLGCAGLVNAARAGNVTLANALGNGVADDKLVYSYVPDLI
                     RYYLGEEPVLPNVDTWRLEDPAHRAEVLDRLAELVLKPVDGSGGKGIVIGPAASAAQL
                     DAVRARIQANPRGWIAQPVVQLSTVPTLVGGQLAPRHVDLRPFAVNDGSSVWVLPGGL
                     TRVALGEGQLIVNSSQGGGSKDTWVLANHEVGDVEPIPAPRARPAAASSVVHPQARSV
                     AIQHSEHSQVEQQQQQQQQRAHQEDPC"
     gene            9051..9833
                     /locus_tag="PLB86_06345"
     CDS             9051..9833
                     /locus_tag="PLB86_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRC64791.1"
                     /translation="MNIVVCVKYVPDAQAERTFNASDSTTDRVNVDGLLSELDEYAVE
                     EALKFVEAGEGEVTVLTIGPDEASDAIKKALQMGADKGVHVIDDAIHGSDALATSLLL
                     AKAIEKIGVPDLIVTGMSSTDGIMGVVPTMLAERLGLPAVTFASELTVGSGTVSARRD
                     GDVASQTIEAALPALVSVTDQINEPRYPSFKGIMAAKKKPVETWTLADLGVSADEVGL
                     DAAWTKVTSFEARPPRAKGTIVKDEGDGGAKLAGFLAERKFI"
     gene            9864..10820
                     /locus_tag="PLB86_06350"
     CDS             9864..10820
                     /locus_tag="PLB86_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRC64792.1"
                     /translation="MAEVLVLVDHVDGALRKTTTELLTIARRLGEPSAVFIGSGFDAA
                     KEGLAAYGAEKVYRIDADLSGFLVVPQAEALAAVAAQASPAAVLVASSPEGKEIAARL
                     AVKLGSGLITDAVDVQSDGAGGVSTTQTVFAGSYTVKATVTKGTPVIAVKPNSAAPEA
                     VAGAAAEVTVPFEPSALAQSAKIVSAKPKEKTGRPELTEAAIVVSGGRGTGGDFSKVE
                     AFADSLGAAVGASRAAVDAGWYPHSHQVGQTGKQVSPQLYVACGISGAIQHRAGMQTS
                     KTIVAVNKDEEAPIFELVDFGVVGDLFAVLPQATDAVAAAKA"
     gene            10924..11466
                     /locus_tag="PLB86_06355"
     CDS             10924..11466
                     /locus_tag="PLB86_06355"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRC64793.1"
                     /translation="MDLLLLRHQPTLWGLAVRLDPMGPEHYDGLRPLFVGDSDSPLAF
                     VLAEKVRVGLAVAKDRPDRADWAIVRIADQRVVGEVVLFSLDADHDAMEFRIALVGPE
                     EFGQGYGTEATRLVRDFAFGPLGLRRVSLEVGASNARARRVYDRAGFIVEGVRRQVRL
                     VDGVWDDVVDMAMLASDPRP"
     gene            complement(11499..12311)
                     /locus_tag="PLB86_06360"
     CDS             complement(11499..12311)
                     /locus_tag="PLB86_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HRC64794.1"
                     /translation="MTHTGPSGTRIAAFFDLDGTLIPGSANIPLAKAAFGAGLVSKRE
                     LALDLARNASFMLRGASDDSSAKVRDRILRGVKGHPGSEVERLADGFLDDLVATITPT
                     MREVLAQHRAEGHELVLVSASPTEIVLRFAVAAGMDYGVGTTAGRDEAGCFDGTLAGP
                     FCYKEGKAEVIRGLAATHGYDLAQCFAYSDSVSDEPMLAIVGNPVAVNPDHELRLMAL
                     AGGWRIVEVGRLRVAVNRTRRAARSAVTSPGKVWTRGRAELTRRREPIADSE"
     gene            12468..>13543
                     /locus_tag="PLB86_06365"
     CDS             12468..>13543
                     /locus_tag="PLB86_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRC64795.1"
                     /translation="MPVYLDHAATTPVFPDVAAAMSAQRDRVGNASSLHTAGRAARRV
                     VEESRERLAGVLGARPSEVVFTSGGTESDNLAVAGVYRARRDADPRRRRLLVSAIEHH
                     AVLDCVQALVDREGAEVDWLPCDADGIVQVADVRAAIAEDPQSVALVSVMWANNEVGT
                     VQDVAAIAAEAHAHGIPMHTDAVQAVGHLPVDFLASGVDLLTLTGHKVGGPIGSGALL
                     VRREQDVVPLVYGGGQERQIRSGTLDTVGILGLSLAVEASVGLLPVEGPRLCRLRDDL
                     IEGAMGLGYDIRPGGMWRPGDWASRSAGNAHLLVPGCEGDSLLYLLDAAGVECSTGSA
                     CQAGVPQPSHVLLAMGLSEREARGA"
BASE COUNT         2038 a         4423 c         4911 g         2071 t
ORIGIN      
        1 gtggcttggg cgggtcgggg ctcggatgtt cccaaggtcc tcacactcgc cggtggtctg
       61 ggcgaggggt atgccggttg gccccgaatt cctcacaccc gccggtggtc tgggcgggtc
      121 ggggctcgga tgttcccaag gtcctcacac tcgccggtga tctgggggag gggtatgccg
      181 gttggccccg aattcctcac acccgccggt gatctgggca gatccaggct cggggcggat
      241 tcacgggtcc ggcatccgcc cggaccagcc ccggacgttg aggcgtcgag gcgtcgacgc
      301 gtcaggccga gaggctcggc tggctcggga agtgggcgaa ccagtcgggg cgccgccgga
      361 ctcggaggcc gccagccgcg aggaggctct ccgcgcggtc gagcgtcgtc ccgggcgtgc
      421 ggtaaatgtc gggagcagtc agcacgagga accgccagcc ctccgcctcc aactcttccc
      481 ggcggcggat gtcgtaggtc cactgatcct gtcggttgat gtggtgacgc ccgtcgtatt
      541 cgacggcgac cttcgcggac ggccaggcca ggtcgatccg atagcgcagc ctgcctgcgt
      601 cgtcgaacac ctcgacgttg accacgggtt cggggaagcc ggccagcacg aagagcatgc
      661 gcacccgggt ctccatggga gagtccaccc cggggcgcac gtatccggca gccagccgcg
      721 cgagagccgc gccgggacct tcgtgctggc gtgccgcttt gaggagcgcg ggcgcggtga
      781 tgtgaccctg gcggaccagc ccgtcgccta ggaccacgag gtcgacgagc tcgagacatc
      841 gagccaggtc gaggaacgtc tgcgcggcgt ccgtgcacgg caggggtgac tggggcgcga
      901 gagccggcgg gacggcatac cggcgcagtt tgacctggcg gcgcacgctc atggcgccct
      961 ccgttgtccc gaggtgggtc cacgcgctgt ccggcacggt cgccccgagg agggtggcgg
     1021 ccgtgtgatg ggaggcgaag caggggtgcg tcgtcgtgcg catcgcggcg agcactcgaa
     1081 tccgcggagt gatctcgact gccgcgctga tgtagatgcc gtgcagcaat cgacggaacc
     1141 tcgggcccct gaggtcctcg tcggtgagcc ccgcgagcag cgcgtctcga cggcggaacg
     1201 ggcggtcgag acggaggtcg atcggggtcc gccgggcggc gggctcggtg ctggttgagg
     1261 tcaaggtcca cactcctgcg gtcgcggtgg ctcctgacga caatgcccga ggttggggag
     1321 tgggtgcagg agttatccac agggggcggc ggcgcgctcg tggggcgtca gacgcggtca
     1381 ccaaggcggc gcgaacacat cgccggcggg tgtggcgaag tcggggcagg gaaggcctgt
     1441 ggaggcccac atcgccggcg agtgtgagga atttgggaaa aggggggcgg ggggcgggaa
     1501 ggggtgggca gggggtggga aggggagggt gtcaggtcgg gtcgacggcg cggtagatgc
     1561 gcatcgaggc cggcagcagg acggcgggct cgtcggggga cacccaggcg gcctgcgcgg
     1621 gcggccggtc ccaggcgctg tcccacagca gttcgtatgc cgtgagcccg ggtgggcgcg
     1681 gcagggtcac ggtcccctcg tgggcatcgc catggaagac cacgagcagc gactggtgcc
     1741 cgtggtgggc gccgtcgaga agcatctgca gggtccggac gcgagcgtca ccccagcgat
     1801 ccatcgtcat gggatgtccg tcggcgccgt accactcgag gtccttggac ccgtcgagat
     1861 ggaccggccc gccactgaag aagtcgcgct ggcgcaacac cgggtgctgg gcccgcaact
     1921 ggatgaggaa ctgcgtcgtg gccagcaggt cggcctgcca gggggcgaga tcccagtcgt
     1981 accaggaggt gtcgttgtcc tgacagaagg cgttgttgtt gccgtgttgg ctccgaccga
     2041 actcgtcgcc gccgttgagc atcggcaccc cggtcgacac gagcagggtg gccaacaggt
     2101 tgcggatcga gcggtgccgc agcatcgaga cgccgtcgtc gatctggcct tcgatgccgt
     2161 ggttccacga ggagttgttg tcggcgccgt cgcggttgtc ctcgcggttg gcttcgttgt
     2221 gcttgtggtt gtatgccgtg aggtcggctg tcgtgaaacc gtcgtgtgcc gcaacgaaat
     2281 tgatcgaggc gatcgtgcct cggtcacggt ggtggaagag gtcggccgat ccggcgagcc
     2341 gggttgccag gtcctgcacc ccgtgccccg gctgaccggc gaggttgcgg gcgaggtcag
     2401 tgagccagaa ggagcgcacc cggtcgcgga accggtcgtt ccactcagag aacagcgggg
     2461 ggaactgccc cgtccgccag ccctggatgc ccaggtccca cggctcggcg atgttcttca
     2521 cctgcgagag caccgggtcg gtgcgcagcg cgatgagcaa cgggtgatcg tggtcgaact
     2581 cgtcgttctt gccgcggccg agcgcgacgg ccaggtcgaa gcggaacccg tcgacgtggc
     2641 actcctgcac ccagtagcgc agcgagtcga ggatcatccg gaccgcgacg acgtggcgca
     2701 cgtcgacggt gttaccgcag ccggtgacgt cgatgtcgtc cccgcgcccg tcaagccggt
     2761 agtaggcgcg attgtccagg cctcgccagg acagcgtcgc gccgcgcgcc gactgctcgg
     2821 cggtgtggtt gtagacgacg tcgaggatga cctcgatgcc ggccgcgtgc agcagtttga
     2881 ccatgccctt gaactcatcg agggcccctt gcgggtcgcg cgcggcggca tacctcgcgt
     2941 ggggggcgaa gaagccgagc gtgttgtagc cccagtggtt gatcagcccg cggcgccaca
     3001 cctccggctc ggtcgtgaac gtatgcaccg gcagcaactc caccgccgtg atcccgagcc
     3061 gcgtgaggta gtcgatggtc gcggggtggg cgagaccggc atacgtgccg cgcaggttcg
     3121 ccggcactcc gggcaggcgc atcgtgaggt tgcgcacatg cgtctcgtag atgaccgatt
     3181 cggtcaacgg ccgtcgcagc gggcggtcgt cgccccaatc gaaacctccg tcgacgacga
     3241 ccgaacgcgg gacgtatgcc gcggagtccc gctcgtcgat cgcgtagaga tcctcggggc
     3301 gcaggtccga ggacgtgcgg tggccgtaga cctcaggaac ccaggtgacc tcgccctcga
     3361 tagcgcgggc gtagggatcg agcagcagtt tggccgggtt gtagcgcatc ccgtcatgcg
     3421 gggcccaggg cccggagacc cggaacccgt accgctggcc cacgcccatg ccgggcacga
     3481 acccgaacca gtagccatag acgcgctcgg tcaggggaac ccggcgttcg gtctcgccgt
     3541 ggacatctcc cggctcgaag aggcacacct gcacgccatc ggcgtgaccg gcatacacac
     3601 acacctgggc gccgccctcg accagtgtca ctcccggggg cggcgggtgg tcgcgactcg
     3661 tgagagaccg atgcaacatg ccctccagcc taggtggaca ctacggttgt ggcatgacgg
     3721 atcccaccgc cctgcccaat ttcccgccgc cctcggacga gcatccgctg cgagggcggg
     3781 tgcttgacgc cctgcaggac ctgggcatcg agccggtcat cgactccgac ggcgacgtcg
     3841 ccttccaggt cggcgagccc tcgcaacaac tgttcgtccg ctgccaggac ggggacttcc
     3901 cggtgatgcg gatcttcggc caatggcaga tcggagcgcc ggcgcccgag gacgtcctgg
     3961 cccgcctgat gcgctgcaac gaggtcaacc tcgagtggca ggtcgccaag tgcggcgtcg
     4021 ccggcgacaa cctcatcgtc acgtgtgacc aggtcgtcat gcctggggcc gacctcggcc
     4081 aactcatgca gttgtcggtg acgctcgtgc tgcaggtcgt cggcgcgtgg tacggatcgt
     4141 gggatcaagg gcgcgacgag gcgccgggag caggaggcag tgatgggtga gttcgtgcgg
     4201 ctcgaggtcg ccgatggcat cggaacgatc cggctggatc gtccgaagat gaacgcgctg
     4261 aacctgcagg tgcagaccga gatcgaggag tgcgcccgcg aggcgaccct gcgtgacgac
     4321 gtcgcggcgg tcatcgtcta tggcggtgag cgggtcttcg cggccggcgc cgacatcaag
     4381 gaaatgcagt cgatgtccta caccgacatg gcggtcgcgg tcgaaggcct gcactccttc
     4441 accacgacgc tggcccggat gcccaagccg agcgtcgccg cgatcaccgg ctatgccctg
     4501 ggcggcggct gcgagttggc gatgtgttgc gacttccggc tcgccggcga gtcggctcaa
     4561 ctgggccagc ccgagatcct gctgggcatc atccctggcg cgggcggcac ccagcgactc
     4621 acccgcctgg tcggcccggc caaggccaag gacctcatct actccggtcg cttcatcaag
     4681 gccgacgagg cactcgcgat tgggctcgtc gaccgggtgg tcccggatga ccaggtgtat
     4741 gccgccgcgc gcgagtggat gtcacagttc gtcggtggtc cgcggtttgc tctcaaggcg
     4801 gccaaggagg ccatcgaccg aggtggcgag ctcgacctcg actccgggct ggccctggag
     4861 aagtcgctct tctcggcctt gttcgccacg cacgaccggt tcgtcggcat ggagtccttc
     4921 gtcgtcaacg ggccgggcaa ggcgcagttc gaaggccggt gagacggcgc gcagacggcc
     4981 tggacgtcct ggtcgtctat gtcccgcggg cgtcgaccga ggcgctgttg gctgccctct
     5041 tcgcggccgg agccggcgcg gtcggtgact atcgcgagtg tgcctgggtg acgtcgggcc
     5101 tgggccggtt ccggcccctg cctggcgcca accccgccgt tggcgatgtc ggggagctcg
     5161 tcacgatcga ggaggaccgc tgcgaggtgg tcatccccac gggtcgtcgc gacgaggtga
     5221 tcgccgccct ccgagcggcc cacccctacg aggagccggc cttccacgtc atcgagaccg
     5281 ccccgctgga ctgacgtcag gacagccggg tcatctcgac ggagacgaac atgtccgagg
     5341 tccggctccc ggtgaagatg ccggtcaacg gcgccacgtc gcgatagtcg cgcccgacgg
     5401 ccacctcgac gtgcaggtcg ccggggcgga tgttgttcgt cgggtcggcg cccacccagc
     5461 cgtcgtccca gaactggatc cacgcgtgcg actccccggc ataggggacg ccgagctcac
     5521 ccgagcggct gggcatgagg tagcccgaca cgtagcgggc cgggatgccc accgatcgca
     5581 gggcccccaa cacgaggtga gccatgtcct gacagacccc agagcgctgt tcccaggcct
     5641 ccgaggcccg ggtgtggact ccggtggcac cgaagacgta cttcacctcg gcgtgcacga
     5701 gatcgacgac cgccacggcg agttcccagg gcgtggcgct ggtccggcgc agttcggcaa
     5761 ccgcagcgag caggtcgggc ggaggcgcga cccggtcgcc gatctcgagc atctcgcagt
     5821 gcgtgtcggt gacgtcgagg ctggtcaggt cgccccactg aaggcgcggc tcctcggagg
     5881 tatgccgttg cacgtcaacg gtgctcgtcg cgacgacgtt gagcgactca tgccgctcga
     5941 acacctcgaa cgccgtgacg gtggtgcccc agtaatcggt gtaggtgtag gtccacggca
     6001 ccggcgagat gtcgatccgg ctgtgcacga cctgctgctc gtgggtggag cgggggagca
     6061 tgcggatctc gttgaacgac gcggtgacac caccggcata cgaatagcca gtgcggtgga
     6121 cgatgcggat ggtggcgctc actgggctcc accgcgccat tcggcggcaa cggcaccctc
     6181 gaagtatcgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgctcactgg
     6301 gctccaccgc gccattcggc ggcaacggca ccctcgaagt atcgcgccga gatcgcggcg
     6361 ttggcgcggg cgcaggtgat ctgcaactcg gccatccgtt ccggcaggtc gaggagcacc
     6421 tctccgcgag tgcgatattc gagctcggcg cgggcccggc ccagggtgcg ggcgacctcg
     6481 tcctcggtca ggccgcgacg ctcgggcggg gtgagccgat cgagggcatc catggcggtg
     6541 tcgagggtgt ggacgatcga gcgcgggaag agccggtcca gcaggaggaa ctcagcggcc
     6601 tcactgtcgg agtcctggtc gtggtggccg cggacgaagg cgtggtgggc cccgcaggcg
     6661 cgcaacgtgc tcgaccacgc agtggccgca ggcccggtgg cggcggacgc cgcgagacgt
     6721 gccgtcatgt cgatgcgttc caaactgcgg cccaggacga ggaagtacca cccctggtcg
     6781 tgggacatcg tgccgtcggc ggtcgccgtg atgacggcgc accgctcccg cacccaccgg
     6841 aacgcgagcg cgggacgcac cttcgcgagc tcgccgcgct gcacggcata ccaggtggtg
     6901 ttgatcgcct cccacatctc ggtggacacg gtctcacggg agcgccgggc gctctcgcgg
     6961 gcgcccttga gggccgagac gatcgacgcg ggtgagtcgg ggtcatgaca gaggcgacgc
     7021 agcacgaggt cctcgctcgc ggggccctcg tggtcgatcc ccatgacggt gagcaaccga
     7081 tcggcggacg cccgggcgtc gatgacggga tcctcgacgg acaggtgcaa ctggacgtcc
     7141 agaaggcgcg aggtgtcctc ggcccgctcg aggtagcggc cgatccagta gagcgactcg
     7201 gcgatgcggc tcagcatggg tcctcctggt gagcgcgctg ttgctgttgc tgttgctgtt
     7261 gctcgacctg ggagtgttcg gagtgctgga tggcgacgct gcgggcctgc ggatggacga
     7321 ccgatgacgc cgccgccgga cgggcgcggg gtgccgggat cggctcgacg tcgccgacct
     7381 cgtggttggc cagcacccag gtgtccttgg aaccgccgcc ctggctggag ttgacgatga
     7441 gctggccctc gccgagagcg acgcgggtca gccctccggg cagcacccac acggacgacc
     7501 cgtcgttgac cgcgaaggga cgcaggtcga cgtggcgagg cgccaactgc cctccgacga
     7561 gcgtcggcac cgtcgagagt tgcacgacgg gctgggcgat ccacccgcgg gggttggctt
     7621 ggattcgggc ccgaacggca tcgagctggg cggctgacgc cgcaggaccg atgacgatgc
     7681 ccttgccgcc cgagccgtcg accggcttga ggaccaactc cgcgaggcgg tcgaggacct
     7741 ctgcccggtg cgcggggtcc tccagacgcc aggtgtcgac gttgggcagc accggctcct
     7801 cacccaagta gtagcggatg agatcgggga cgtaggagta gacgagtttg tcgtcggcga
     7861 cgccgttgcc cagggcgttg gccagggtga cgttgccggc gcgagccgcg ttgaccagcc
     7921 ctgcgcagcc cagcagcgag tcgccgcgga agtgcaccgg gtcgaggaac tcgtcgtcga
     7981 ccctgcggta gatgacgtgg accggccgta gcccggtcgt cgtgcggacc atgacccgcc
     8041 cggcctggca caccaggtcg cgtccctcga cgagccgaca gcccatcatt cgggcgagga
     8101 gggcgtgctc gaagtaggcg gagttgtaga cgcccggggt gagcacgacg acggtcgggt
     8161 ccgacacgcc gctcggcgcg ctggcccgca gcgcggcgag cagacgggcg gggtagctgc
     8221 ggaccgggcg gatccggtgg tcggcgaaga cctcaggcag ggcgctggtg agggcccggc
     8281 ggttggtcat gacgtaggag accccggacg ggatccgtac gttgtcctcg agcacccgga
     8341 accggccgtc gccgtcgcgc acaaggtcga tgccgctgac gtgcacccga accccgttgg
     8401 ccggggtcag cccggcgacc acccgatgaa agtgcggcga actcgtcacc acggtccgcg
     8461 gcatcacctc gtcgttgaag aggtcgcccg gcccgtagac gtcgtcgagg aaccgctcga
     8521 gggcccgcac ccgttgagtc agccccacct cgaccgtcgc ccaggtgtcg gcgtcgagca
     8581 accgcggcac gatgtccagc gggaacggcc gttcctcgcc gccgatgtcg aaggtcacgc
     8641 cctgctcgac atagatgcga gccaggaagt ccgcacgccc acgcacgtcc gcaggactca
     8701 tccgggtcaa ccggttgtgg acgtcggtgt accccgatcg aacctcgttc ggcgccgcct
     8761 cgaacatctc gtcgaaggtg cgctcgcggg cggcataccc ggcgaatagc gtgtccacga
     8821 gggaggacgt tagggccgaa cgatttccaa ctcgtttcgt ccgcgttccg tcgtggagac
     8881 atcgcccgcg caggtatgcc gtgccgacgg tcacgtgggt cgcgcgtgag tcgcgtcacg
     8941 cggcataccg tgaggtggtg ggcatcacgc ccagggcggc ggggagtgtc ggcctgacct
     9001 gacaggatgg tccagaccct aaccagcaca cgggaagagg acgtgtctag atgaacatcg
     9061 tcgtctgcgt gaagtatgtg cccgacgcgc aagcagagcg caccttcaac gcctccgaca
     9121 gcacgaccga tcgcgtcaac gtcgacgggt tgctctccga gctcgacgag tatgccgtcg
     9181 aggaggcgct caagttcgtc gaagcgggcg aaggtgaggt cactgtcctc accatcggac
     9241 ccgacgaggc atccgacgcg atcaagaagg ccctgcagat gggcgccgac aagggagtgc
     9301 acgtcatcga cgacgccatc cacggctccg acgctctcgc gacgtcgctg ttgctggcca
     9361 aggcgatcga gaagatcggc gtgcccgacc tcatcgtcac ggggatgtcc tcgaccgacg
     9421 gcatcatggg cgtcgttccg acgatgctcg ccgagcgcct cggcctgccg gccgtgacct
     9481 tcgcgtccga gctcaccgtg ggttccggca ccgtgtcggc gcgccgcgac ggtgacgtcg
     9541 ccagccagac catcgaggcc gcgctgccgg ccctcgtgtc ggtgaccgac cagatcaacg
     9601 agccgcgtta cccctccttc aaggggatca tggcggcgaa gaagaagccc gtcgagacgt
     9661 ggacgctggc tgacctgggc gtgtccgccg acgaggtcgg cctcgacgcc gcctggacca
     9721 aggtgacctc cttcgaggcg cggccgccgc gcgcgaaggg caccatcgtc aaggacgagg
     9781 gcgacggcgg agccaagctc gccggcttcc tcgccgagcg caagttcatc tgagcggcga
     9841 cgcgggaata ggagatcact gacatggctg aagttctcgt cctcgtcgac cacgtcgacg
     9901 gcgcgctgcg caagacgacc accgaactgc tcaccatcgc ccggcggctc ggcgagccgt
     9961 cggccgtctt catcggatcc ggcttcgatg ccgccaagga ggggctggcg gcatacggtg
    10021 cggagaaggt ctaccgcatc gacgccgacc tgtccggctt ccttgtggtg ccccaggccg
    10081 aggccctcgc cgcggtcgcc gcccaggcgt ccccggcagc ggtgctcgtc gcgagttcac
    10141 ccgaaggcaa ggagatcgcc gcgcgcttgg cggtcaagct gggctccggg ctcatcaccg
    10201 acgcggtcga cgtgcagtcc gacggagcag gcggggtctc gacgacccag accgttttcg
    10261 ccgggtccta caccgtcaag gcgaccgtca ccaagggcac gccggttatc gcggtcaagc
    10321 ccaattcggc tgcgcccgaa gcggttgctg gtgctgctgc cgaggtgacc gtccccttcg
    10381 agccgtcggc gctggcccag tcggccaaga tcgtgtcggc caagccgaag gagaagacgg
    10441 gtcgccccga gctcaccgaa gctgccatcg tcgtctcggg cggacgtggc accggtggcg
    10501 acttctccaa ggtcgaagcc ttcgccgact ccctcggcgc agcggtcggt gcgagccgcg
    10561 ccgctgtcga cgccggctgg tacccgcaca gccaccaggt cggccagacc ggcaagcagg
    10621 tcagccccca gttgtacgtt gcgtgcggca tctccggtgc gatccagcac cgggccggca
    10681 tgcagacctc gaagacgatc gtcgccgtca acaaggacga agaggcgccg atcttcgagc
    10741 tggtcgactt cggagtggtc ggcgacctgt tcgccgtgct cccgcaggcg accgacgcgg
    10801 tggcggcggc caaggcctag tcgcgcacgc ggcatacgct gcggggtccg gaccggtggt
    10861 ggtccgggcc ccgcttccgt atgccggctt gccgccgcgc gctgggattg gttgactccg
    10921 cccgtggatc tcttgctgtt gcggcaccag ccgaccctgt ggggcctggc cgtacgcctc
    10981 gatccgatgg ggcccgaaca ctacgacggg ctgcggccct tgttcgtggg cgactccgac
    11041 agcccgttgg cgttcgtgct ggccgagaag gtccgcgtcg ggctggccgt cgccaaggac
    11101 cgacccgatc gggccgattg ggccatcgtg cggatcgccg atcagcgggt cgtgggggag
    11161 gtcgtgctgt tctccctcga cgccgatcac gacgcgatgg agttccgcat cgcgctcgtc
    11221 ggcccggagg agttcggcca gggctacggg acggaggcga cccgtctggt gcgggacttc
    11281 gccttcgggc cgctggggtt gcgccgggtg agcctggaag tcggcgcgag taatgcgcgg
    11341 gcgcggcggg tgtatgaccg ggcgggcttc attgtcgagg gagtgcgccg tcaggtgcgg
    11401 ctggtcgacg gggtgtggga cgacgtggtc gacatggcga tgctggcctc ggacccgagg
    11461 ccctgaaaga cccgaggccc tgaaagaccc gagggcttct actcggagtc ggcgatgggc
    11521 tcgcgtcgcc gggtcaactc agctcgcccg cgggtccaca ccttgcccgg cgaggtgacc
    11581 gccgaacgag ccgcccggcg ggtccggttg acagcaaccc gcaggcggcc gacctcgacg
    11641 atccgccaac cgccagccaa tgccatcagc cgcagttcgt ggtcggggtt gacggcgacc
    11701 gggttgccga cgatcgccag catcggctcg tcactcaccg agtccgaata cgcgaagcac
    11761 tgcgccaagt cgtagccatg ggtcgccgcg agcccgcgga tgacctccgc cttgccctcc
    11821 ttgtaacaga acggcccggc cagggtgccg tcgaaacacc ccgcctcgtc ccgaccggca
    11881 gtggtcccca cgccgtagtc catgcccgcc gccaccgcga aacgcaggac gatctcggtc
    11941 gggctcgccg acacgaggac gagctcgtga ccctcggcgc gatgttgggc gagcacctct
    12001 cgcatcgtcg gggtgatcgt ggcgaccagg tcgtcgagaa agccatcggc gagccgttca
    12061 acctcggagc cagggtgtcc cttgaccccg cgcaggatcc ggtcgcggac cttggccgag
    12121 ctgtcgtcac tggcgccgcg caacatgaac gacgcgttgc gcgccaggtc gagggccaac
    12181 tcccgcttgc tcaccagccc cgcgccgaag gcagccttgg cgagcggaat gttggccgac
    12241 ccgggaatga gcgtgccgtc caggtcgaag aacgccgcga tacgcgtgcc agaggggccg
    12301 gtgtgggtca tgctcgctga gctcctcggg acggggacga accagcacat catgacgcag
    12361 gtcgcggttg cttgccaggg ttctccctcc cctttccgcc ccgtgaccga ccgtccacgg
    12421 tatgccgcgt cgcggcggca tactcacgcg cggcttagga tcggcgggtg cccgtgtacc
    12481 tcgaccatgc cgcgacgacg ccggtcttcc cggacgtcgc ggctgcgatg tcggcgcagc
    12541 gggatcgggt cggcaacgcc tcgtcgttgc acactgcggg ccgggctgcc cggcgggtcg
    12601 tcgaggagtc ccgcgagcgg ttggccggag tgctcggggc tcgcccgtcc gaggtcgtgt
    12661 tcacctctgg ggggaccgag tcggacaacc tcgccgtggc gggcgtctat cgggctcgcc
    12721 gagacgcaga cccccggcgg cgtcggctgc ttgtcagcgc gatcgagcac cacgccgtgc
    12781 tcgactgcgt gcaggccctg gtcgaccgcg agggcgccga agtcgactgg ctgccctgcg
    12841 atgccgatgg catcgttcag gtcgccgacg tgcgcgccgc catcgcggaa gacccgcagt
    12901 cggtcgcact cgtctcggtg atgtgggcca acaatgaggt cggcaccgtg caggacgtgg
    12961 cggcgatcgc cgcggaggcg cacgcgcacg gcataccgat gcacacggac gcggtccaag
    13021 cggtcggtca cctacccgtg gacttcttgg ccagcggcgt cgacctgctc accctgaccg
    13081 ggcacaaggt gggcgggccg atcgggtccg gcgctttgct cgtgcggcgg gagcaggatg
    13141 ttgtgccgct ggtctacggc ggcggccagg agcgccagat ccgttccgga accctcgaca
    13201 cggtcgggat cctggggttg tcgctcgcgg tcgaggcgtc cgtggggctg cttccggtcg
    13261 agggaccgag gctgtgtcgg ttgcgtgacg acctcatcga gggcgcgatg gggctgggtt
    13321 atgacatccg gcccggtggg atgtggcggc cgggcgactg ggcgagccgg tcggccggca
    13381 acgcgcacct gctcgtcccc ggatgcgagg gtgactcgtt gctctacctg ctcgacgcgg
    13441 ccggggtcga gtgctcgaca gggtcggcct gccaggccgg ggtgccgcag cccagccacg
    13501 tcttgttggc catggggttg agcgagcgcg aggcgcgggg agc
//
LOCUS       DAORPW010000055        13417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_945_length_13417_cov_689.460410, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000055 DAORPW010000000
VERSION     DAORPW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13417
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_945_length_13417_cov_689.460410"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLB86_06370"
     CDS             complement(<1..557)
                     /locus_tag="PLB86_06370"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HRC64796.1"
                     /translation="MTGSLGSGAVTITPLHGIPEIAEGDDLAAVLAAACVRAGVPLAE
                     GDILAVSSKIVSKALGLTAPTADKEAVVAAETVRVVAERAGASGITRIVQGKAGPVMA
                     AAGVDASNTGGRDVLLLLPHDPDAVCRDLHAALRAATGVEVFAVVLTDTAGRPWREGQ
                     VDFALGSHGLRVLDDLRGSVDADGRA"
     gene            complement(554..1528)
                     /gene="cofD"
                     /locus_tag="PLB86_06375"
     CDS             complement(554..1528)
                     /gene="cofD"
                     /locus_tag="PLB86_06375"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HRC64797.1"
                     /translation="MRVTALSGGVGGARFLRGLLAHIDRTPELAGSTVTVIGNTGDDI
                     TLFGLRVCPDIDTVLYTLGGGVHEAQGWGRSDETYAVHGELGALGVPPDWFSLGDKDF
                     GTHIARSLWLGQGLSLSQVTARLAQRWGLPDRGVTLLPMTDTPVETHVVVDEGEGPRA
                     VHFQEWWVRMRAAIPAERFVVAGMDRATPAPGVLDAIRTADVVLLPPSNPVVSIGIIL
                     GVPGVRDALRGTRAPVVGVSPLVSGRPVRGHADACLSAIGIPATSAAVATLYADFLDG
                     WLVDDSDADVAMPDGVRTLARPLLMTDIPAAADIAGAALDLGRDLRGR"
     gene            1788..2051
                     /locus_tag="PLB86_06380"
     CDS             1788..2051
                     /locus_tag="PLB86_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRC64798.1"
                     /translation="MHELLLVPMDGFQDEEMGWQERSLCAQTDPEAFFPEKGGSTREA
                     KRVCVGCEVRVECLEYALANDERFGIWGGLSERERRKLKKRAV"
     gene            2095..5361
                     /locus_tag="PLB86_06385"
     CDS             2095..5361
                     /locus_tag="PLB86_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC64799.1"
                     /translation="MTAATPDPAVSTPTDPTPTASEPTPVARRPFVTAVALCRSGVDS
                     LTVTLAALADQTRRVDRIVVLDLTQGAADLGQVTRTQSGADTGLPEAELVSEPTAGPV
                     GAAVNRLLQTLAQAPDPAAEWWWLLPDDVVPEPAALAKLVDAVRRSPSVGMAGPKVLD
                     RNRPRLLVEVGHQLTRSGRRIAAPGRGEPDQGQYDTRTDVLAVGLPGQLVRREVLTQV
                     GGFEPAFAGPGEALDLGWRAQLAGERVVVVPAARVLAGPETSTTETPQGPEPTVPAET
                     MSERRAARRAARRVALTRCSPWAVPGLALWVLLSSIGSALVLLLLKRPAHAWVELADL
                     GALVRPFSSIAARWRFRTRRRLRRRDLATLFVTSGESLRHTADRVQEALTPDRDGARA
                     GLAAGAGIESGPVAEEAEDLNVLSASLPERVFSNAGVLATLAAGIVAAVGFRSSFRGG
                     LLDARGTGLAGGELARVSTDASGLWHAFRDSWHGAGLGTGADSAPYLGVLATLTWLVE
                     RLPYVGEGRSPASVLLAWILVLGMPVATAAAYLAGRVVTPARWPRALVAFAWGTGGVI
                     AAAVSHGRVTLVLAHILLPLVVAGVVRSARADGTFTAAAAAALGAAVLGAMVPPYLAL
                     VAVAAAVLVIVGPGWGRRVRGLALLVLPPALQGSLLVQLRDPVAWLGAPGALDSAGAT
                     TASWWQLLAGYPEGGSALVVALSASVLLAGVVTLVRRPGSRGAGVAYAALTLLAAVGL
                     GWALLARRVVVGTVLGADGVPVEATPWPGVGLQCFVLAVLGLALLGSVGMRGILGKER
                     WAPRRLLAGAGLLAVTASVAGVAALGGWARLDGIVSVGFDALPAVAVEQARGPEANRM
                     IVLSTSEAAVEYQLVAREPGEILRGLARPSGASDPGVAAAVTALVASQAGDAPGAGAQ
                     LADLGIGFVSVQGSADPEVIRTLDATAGLTRLGNTDDQTLWRVLARPSVTTPTEVVPA
                     SRARITTADGGLSGWVRVDGAHGALTASLPAGSVGRRLVLAEPREWVQHAQVHFDGAL
                     LTATSQGLPTYVLPDRAGVLTVDIPPRDPRWFAGQFALLALTVFLAVPFGNRRSRRIR
                     "
     gene            5358..6848
                     /locus_tag="PLB86_06390"
     CDS             5358..6848
                     /locus_tag="PLB86_06390"
                     /inference="COORDINATES: protein motif:HMM:NF040160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HRC64800.1"
                     /translation="MTPIGWSALRVLGFAAVLGGLVAGSTQLPSRLELTSIAPVVPAD
                     VLSPPSTPASQMTLGCPGPETDGFSGLPAVPGEATSILAATAPSEAMQGIATAPAPGA
                     VAVRSSASAASDPALATSATRGGVVSGDVSGAAAGEVSATGSLAVGLAALQTGLRREG
                     DERGLQSVACQAPRSEVWLLAGGGAPTRRERLVVANAGANPVTVDIQVHGASGPIASV
                     NGARVAVPPHGRVGVLIDAIAPGEASPVVHVTATGGLITAVLEDSWIDGATGRGRDDV
                     GPADGPATEQVIPAAYLTGVATLRIVVPGTDEAVVQSRALSPNGAVPLPADQVVRIPG
                     GSVRDIDLSGLEPGAYALQVRADRPVVAAVVIERRDAGVAQSDLAWVPATAVIPVLAG
                     SPVPAGTVSRLLLAGTAAPWAATVYVVGPSGAVTSIPAQGVADSSWTVDLPAGAAAVW
                     IRPSSGTVRAGISAELADPTGPLVTLVGINPASLTTTAIPVREVRR"
     gene            complement(6857..7288)
                     /locus_tag="PLB86_06395"
     CDS             complement(6857..7288)
                     /locus_tag="PLB86_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRC64801.1"
                     /translation="MNRDDPRAPRTRRRHGRGVRGPLAWPPVPAMRSRAERFDELVMD
                     AATRFEGHWGRPFPAFELGVEDVPPSDPAPWEHEEVPLGRFFASDARREARVVIYRRP
                     IETRAQAPSDLALLVDEVVAEQLAALLGVSPRDLDPGYDGD"
     gene            7390..7785
                     /locus_tag="PLB86_06400"
     CDS             7390..7785
                     /locus_tag="PLB86_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HRC64802.1"
                     /translation="MGLTRQCSKTGCRRSAVATLTYVYADQTAVLGPLATYAEPHTYD
                     LCGQHAERLTVPRGWDVVRLAFDPSPPAPSSDDLLALADAVREAGRPRSRAPLSPPSS
                     PSQSSAPGAAEASPATGARRGHLRALPNN"
     gene            7830..9269
                     /locus_tag="PLB86_06405"
                     /pseudo
     CDS             7830..9269
                     /locus_tag="PLB86_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
     gene            9557..10753
                     /locus_tag="PLB86_06410"
     CDS             9557..10753
                     /locus_tag="PLB86_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRC64803.1"
                     /translation="MPRWDDRLVDDEAERERLDSRGTLRALATAGAQVRQSLGLAQEA
                     GIARVAGGVRPRGVLVAARGGTGVIGDTLQMVAEPGSPVPISTAGTPPLPGWVGTLDL
                     VIAVSESGRAAGPLALAAAAARRGASLLTVGADDSPLAAVCASARGIHVGLPAGATSS
                     RTALWALLTPVLLAADVLGLARTGPDLLEAVADRLDAGAAAYRPSSEAFVNPAKLLAA
                     DLSGGLPVVLGDGAVAGVAARRAAMMLARTARVPATVGELPGGASEVVALLDGPYTRH
                     GAREPDLASAVDDIFRDPFLDGAPDPRLVLLMLRGALAAGMTPAGVGGSMPEDTDDLG
                     DAVATAAMDAGVRVHERRAEPGHPLEQLADLIGLVDFAATYLALGLGLDPGVSAHVRA
                     LRDHGR"
     gene            complement(10834..12462)
                     /locus_tag="PLB86_06415"
     CDS             complement(10834..12462)
                     /locus_tag="PLB86_06415"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRC64804.1"
                     /translation="MDKPFAITLDLGSSLANHTGSWRTEKPLYVDLLPPCNQGCPAGE
                     NIQQWLFHAEEGNYEAAWRQLTQDNPFPAIMGRVCYHPCETACNRAQVDEAVGINAVE
                     RFLGDQALELGWAFEPPAAELLTGKRVLVIGAGPSGLSAAYHLRRLGHEVTVRDAGPM
                     AGGMMRFGIPSYRLPRNILDAEVKRITDIGVTLELNTKVSDLEGALAEGFEAVFLAVG
                     AHIGKRAYIPAGSSAKILDAVSYLHDVETQEEKPMLGRRVVVYGGGNTAIDAARTAKR
                     LGAEEAIIVYRRNRDKMPAHDFEVQEAQEEGILLKWLSTIKHVDEGKMVVEKMALDET
                     GFPQPTGEFEELEADSLILALGQEADLSFLEGVAGITVADGVVAVDGTMMTGRAGIFA
                     GGDMVPAERTVTVAVGHGKKAARYIDGYLRGTAYTPPAKHAVATFEKLTTWYYADAPH
                     QHREHLEGARRASTFDEVVTGLDEETAQFEARRCMSCGNCFACDNCFGVCPDNAVKKT
                     SIGHGYEIDYDYCKGCGMCVAECPCGSIHMVPED"
     gene            complement(12462..>13417)
                     /locus_tag="PLB86_06420"
     CDS             complement(12462..>13417)
                     /locus_tag="PLB86_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRC64805.1"
                     /translation="EVRSVQSDPNRTNSLTSGHRACQGCGEALGARYALDAAMHAADG
                     KIVAVNATGCLEVFSTPYPETSWRVAWLHSLFGNAPAVASGVAAAYKAMGRSDVRVVA
                     QGGDGGTVDIGFGCLSGMFERNDDVLYICYDNEAYMNTGIQRSGATPPAARTATTQAV
                     GEDPGNTFGQGKDLPRIAMAHEIPYVATASVADLRDLEAKVHRAMSMRGARYIHVMVP
                     CPLGWGTASGDTIKVARLATQSGIFPVFEAEYGEVVASSPIRRQVPVEDYLKGQKRFA
                     HLFSPTRRQDVIDRMQAQADRNIRRYNLLPTAGATETKAQD"
BASE COUNT         1697 a         4821 c         4829 g         2070 t
ORIGIN      
        1 gcgcgcccgt cggcgtcgac cgacccgcgc agatcatcaa gcacccgcag cccgtgcgac
       61 cccagcgcga aatcgacctg accctcccgc cacggtcgac cggcggtgtc cgtgaggacc
      121 accgcgaaca cctcgacccc ggtggcggcg cggagcgcgg cgtggaggtc gcggcatacg
      181 gcatcggggt cgtgggggag caggagcagg acgtcgcgcc ctccggtgtt ggaggcgtcg
      241 acccccgcgg cagccatcac cggtccggcc ttgccttgca cgatccgcgt gatgccgctc
      301 gcccccgcac gttcggccac cacccggacc gtttcggcgg cgacgaccgc ctccttgtcg
      361 gcggtcgggg ccgtgaggcc gagcgccttc gacacgatct tgctcgacac cgcgaggatg
      421 tcgccctcag ccagtggcac cccggcccgc acgcacgcgg ccgcgagcac ggctgcgagg
      481 tcgtcccctt ccgcgatctc cggtatgccg tgcagcgggg tgatcgtcac cgcgccggat
      541 ccgagcgagc cggtcatcgc ccgcgcaggt cgcgaccgag gtcgagagcc gccccggcga
      601 tgtcggcagc ggccgggatg tcggtcatga gcagtgggcg ggccagggtc cgcaccccgt
      661 cgggcatcgc cacgtcggcg tcggagtcgt cgaccagcca gccgtcgagg aagtcggcat
      721 agagggtggc gaccgcggcg gaggtggccg ggatcccgat ggcgctcagg caggcatcgg
      781 catgcccgcg caccgggcgg cccgacacga ggggggagac gcccaccacg ggcgctcggg
      841 tgccgcgcaa ggcgtcgcgg accccgggca cgccgaggat gatcccgatc gacacgaccg
      901 ggttgctcgg cgggagcagg acgacgtcag cggtgcggat cgcgtccaac acccctggcg
      961 cgggcgtggc ccggtccatc cctgcgacga cgaaacgctc agcggggatg gcggctcgca
     1021 tccgcaccca ccattcctgg aagtgcaccg ctcgtggccc ctcaccctcg tcgacgacga
     1081 cgtgggtctc gaccggggtg tcagtcatcg gcagcagggt cacgccgcgg tcaggcagcc
     1141 cccagcgctg ggccagccgg gccgtcacct ggctcaggga caggccttgg ccgagccaca
     1201 gcgaccttgc gatatgggtt ccaaaatctt tgtcccccaa cgagaaccag tcgggcggga
     1261 cacccagcgc acccaactcg ccgtgcacgg catacgtctc gtcggagcgt ccccacccct
     1321 gggcctcgtg gacaccgccg cccagggtgt agagcaccgt gtcgatatcg ggacagaccc
     1381 gcagcccgaa gagggtgatg tcgtcaccgg tgttgccgat gacggtgacc gtgctcccgg
     1441 ccaactcagg ggtgcgatcg atgtgggcga gcagcccgcg caggaatcgg gccccgccca
     1501 cgccgccgga caacgcagta acacgcatgg gggaagtctg tcagcccacc gggtgggcac
     1561 ggaccggccg tccacggggt acgccgcagc gcccgcccgg acgtccgaga cgcctgggga
     1621 gcggcgaaaa cccctcaaat atcagattcg gcttgactaa acgggtatga cacgcgtgta
     1681 attccaccag tgtcgttcac acccgaccgg gccgcagcgt ggtctgacag ggcgtcgaac
     1741 ggttgcagga caggtgggtg catgccaacg agggtcgagg aggcgacatg cacgaactgc
     1801 tgttggtgcc aatggacggg tttcaggacg aggagatggg gtggcaggag cgctcgctgt
     1861 gcgctcagac cgaccccgag gcgttcttcc cggagaaggg ggggtccacg cgcgaggcca
     1921 agcgggtctg cgtgggctgt gaggtgcgcg tcgagtgctt ggagtacgcg ctggccaatg
     1981 acgaacggtt cggtatctgg ggcgggttgt ccgaacgcga gcgccgcaag ctgaagaaac
     2041 gcgcggtctg agcgcgccct tgcggccctt ggccgcaacg cctcgaggag tcccgtgacc
     2101 gcagcaaccc cggatcccgc cgtttccacg cctactgatc ccacgcccac cgcgtccgag
     2161 ccgaccccgg tcgcgcggcg tcccttcgtg accgccgtgg ccctgtgccg ctcgggcgtc
     2221 gactcgctga ccgtgacatt ggccgcgttg gctgaccaga cccgccgcgt cgaccggatc
     2281 gtcgtgctcg acctcaccca gggtgcggcg gacctcggcc aggtgacccg gacccagtcc
     2341 ggtgccgaca ccggactgcc cgaggccgag ctcgtcagcg agccgacggc gggtcccgtc
     2401 ggcgcggccg tgaaccggct gttgcagacg ttggcgcagg cacccgaccc ggccgccgag
     2461 tggtggtggc tgctgcccga tgacgtcgtc cccgagcccg cggccctggc caagctggtc
     2521 gatgccgtgc gtcggtcacc gtcggtgggg atggccggcc cgaaggtgct ggaccggaac
     2581 cggcctcggt tgctcgtgga agtgggtcat caactcacca ggtccggtcg ccggatcgct
     2641 gcgccgggtc gcggtgagcc cgaccaggga cagtacgaca cgcgcacgga cgtcctggcg
     2701 gtgggcctgc cgggccaact cgtccgccgg gaggtcctga cgcaggtggg cggtttcgag
     2761 ccggccttcg ccgggccggg tgaagccctc gacctcgggt ggcgcgccca gttggcgggg
     2821 gaacgcgtcg tcgtcgtccc tgccgcccgg gtcctggccg gccccgagac ctcgacgacc
     2881 gaaacgcccc aaggccccga accgaccgtt ccggccgaga cgatgtccga acgtcgcgcc
     2941 gcgcggcggg cggcccgacg ggtcgctctg acccgctgct ccccatgggc cgttcccggg
     3001 ctggcgctct gggtgctgct cagctcgatc ggctcggccc tggtcctgct tctgctcaaa
     3061 cgcccggcgc acgcctgggt ggagttggcc gatctcggcg cgctggtccg gccgttctcc
     3121 tcgatcgccg ctcggtggcg cttccgcacc cgtcgccgac tgcgtcgacg agatctcgcg
     3181 accctgttcg tcacctcggg cgagtcgctg cggcataccg ctgaccgagt ccaggaggcg
     3241 ctcacccctg atcgcgacgg cgcccgcgcc gggctcgcgg ctggggccgg catcgagtcc
     3301 ggcccggtcg ccgaggaggc cgaggacctc aacgtcctct ctgcgtcgct gcccgagcgg
     3361 gtcttcagca acgccggcgt cctggccacc ctggctgcgg gcatcgtcgc ggcggtcggg
     3421 ttccgctcgt cgttccgcgg cgggctgctc gacgcgcgag gcaccggtct cgccggaggg
     3481 gagttggctc gcgtctcgac cgacgcgtcc ggcctctggc acgcgttccg cgattcgtgg
     3541 cacggggcgg gcttgggcac cggtgccgat tcggcaccct acctcggcgt gctcgcgacc
     3601 ctgacctggt tggtcgagcg ccttccctat gtgggcgagg gccgctcgcc cgcgtccgtc
     3661 ctgctcgcgt ggatcctcgt cctcggtatg ccggtcgcga ccgccgcggc atacctcgcc
     3721 ggccgggtgg tcaccccggc ccgctggccg cgtgcgctgg tcgccttcgc ttggggaacc
     3781 ggcggggtca tcgccgccgc ggtgagccac gggcgggtca ccctggtgct cgcccacatc
     3841 ctgttgccct tggtggtcgc cggggtggtg cgctcggctc gggccgacgg gaccttcacc
     3901 gcggcggccg ctgcggcgtt gggggccgcg gtgttgggcg ccatggttcc gccctatctc
     3961 gccctcgtcg ctgtggctgc cgcggtgctc gtcatcgtcg gccccggttg gggacgtcgg
     4021 gtgcgcggcc tggccctgct cgtcctcccc ccagccctgc aggggtcgtt gctcgtccaa
     4081 ctgcgcgacc cggtcgcctg gctcggggcg cctggtgccc tcgactcggc cggcgcgaca
     4141 acggcctcct ggtggcaact gctcgccggc tatccggagg gtggctcggc cctcgtcgtc
     4201 gcgttgtcgg cgtcggtgct gctcgccgga gtggtcaccc ttgtccgtcg cccaggctcg
     4261 cggggcgcag gcgtggcgta tgccgccctc accctgctcg ccgccgtcgg gctcgggtgg
     4321 gccctgctgg ccagacgtgt cgtcgtgggc accgtcctcg gggccgatgg tgtccccgtg
     4381 gaggcgactc cgtggccggg ggtcgggctg cagtgtttcg ttctcgccgt gttgggcttg
     4441 gcgttgctgg gctcggtggg catgcgcggg atcctcggca aggagcggtg ggcgcctcgt
     4501 cggctcctcg cgggagccgg cctgctggcg gtcaccgcat cggtggcggg cgttgccgcg
     4561 ttgggcggtt gggcacgcct cgacgggatc gtcagcgtcg ggttcgacgc ccttcccgca
     4621 gtcgcggtgg aacaggcgcg tggccctgag gccaaccgga tgatcgtcct gtcgacgagc
     4681 gaggccgccg tcgaatacca actcgtcgcc cgtgagccgg gggagatctt gcgcgggctc
     4741 gcccgtccga gtggggcgtc cgacccgggc gtcgccgccg cggtcactgc gctggtcgcc
     4801 agccaggccg gcgatgctcc gggcgccggg gcccaactgg ccgatctcgg catcggcttc
     4861 gtgtcggtcc aaggcagcgc cgaccccgag gtgatccgca ccctcgatgc gacagccggg
     4921 ctcaccaggc tcgggaacac cgatgaccag accttgtggc gggtgctcgc ccgcccgtcg
     4981 gtcacgacgc cgaccgaggt ggtcccggcc agcagggcgc ggatcaccac ggccgatggt
     5041 ggcctgtcgg ggtgggtgcg cgtcgatggc gcccatggcg cgctgacggc ttccctgcct
     5101 gccgggtcgg tcggccgcag gctggtcctg gccgagcccc gcgagtgggt ccagcacgct
     5161 caggtccatt tcgacggagc gctcttgacg gcgacgtcgc agggtctgcc gacctacgtc
     5221 ctgcctgatc gggccggcgt gttgaccgtc gacatccctc cgcgcgatcc ccgttggttc
     5281 gccgggcaat tcgccctcct ggctctgacg gtcttcctcg cagtgccgtt tggcaatcgc
     5341 cgatccagga ggatccgatg accccgatcg ggtggtctgc gctgcgggtc ctcggcttcg
     5401 ccgccgtgct cggcggactc gtggccgggt ccacgcagct tcccagccgg ctggagttga
     5461 cctcgatcgc ccccgtcgtc cccgctgacg tgctctcccc gccgagcacc ccggcgagcc
     5521 agatgacgct cgggtgcccc ggccccgaga cggatggctt ctcggggctg ccggccgtcc
     5581 cgggcgaggc gacaagcatc cttgcggcca ccgcgccgag cgaggcgatg caggggatcg
     5641 ccaccgctcc ggcgcccggc gccgttgcgg tccgctcatc ggcctcggcg gcctctgacc
     5701 cggcgctcgc gacgtcggcc acccgtggcg gcgtcgtctc gggcgacgtg tcgggcgcgg
     5761 ccgccggcga ggtcagcgct accggctcgc tcgccgtggg gctggcggcc ctgcagaccg
     5821 ggctgcgccg cgagggcgac gaacgcggtc tgcagtcagt ggcttgccag gcgccccgct
     5881 cggaggtgtg gctgctcgcc ggtggtgggg ctcccacgcg ccgcgagcgg ctggtcgtgg
     5941 ccaacgccgg ggccaacccg gtgacggtcg acatccaggt gcacggtgcg tccggtccga
     6001 tcgcgtcggt caacggggcg cgggtcgccg tgcccccaca tggccgcgtg ggcgtcctca
     6061 tcgatgccat cgcccccggc gaagccagcc cggtcgttca cgtcactgcc accggtggcc
     6121 tcatcacggc ggtgctcgaa gacagctgga tcgacggggc gaccgggcgt ggtcgtgacg
     6181 atgtcgggcc agccgacggc ccggccaccg agcaggtgat cccggccgcc tatctcaccg
     6241 gcgtcgccac cttgcggatc gtcgtccccg ggaccgacga ggccgtggtc cagtcgcgtg
     6301 ccttgagccc caacggcgcc gttccgctgc ccgccgacca ggtcgtgcgg atcccgggcg
     6361 gcagtgtccg tgacatcgac ctgtcggggt tggagccggg cgcctacgcg ctgcaggtcc
     6421 gcgccgatcg cccggtcgtg gccgcggtcg tcatcgagcg gcgcgacgcc ggtgtggcgc
     6481 agtccgacct ggcctgggtc cccgccacgg ccgtcatccc cgttctggcc ggctctcccg
     6541 tgcctgccgg caccgtcagc cgactgttgc tcgcgggcac cgctgcgcca tgggcagcca
     6601 cggtctacgt cgtcggcccc tcgggcgcgg tgacgtcgat cccggcccag ggggtggccg
     6661 actcctcgtg gacggtcgac cttccggccg gggcggcggc cgtctggatc cggccgtcct
     6721 ccggcacggt ccgtgccggg atctccgccg agctcgccga ccccaccggg cccttggtga
     6781 ccctcgtcgg gatcaaccca gccagcttga cgacaaccgc catcccggtg cgcgaagtcc
     6841 gccgctgacg gcgcgctcag tcgccgtcgt agccaggatc caggtcgcgc gggctcacac
     6901 cgagcagcgc agccaactgc tcggcgacga cctcgtcgac gagcagggcc aaatccgagg
     6961 gcgcctgcgc gcgggtctcg atgggtcgcc gatagatgac gacgcgcgcc tcgcggcggg
     7021 catcggacgc gaagaaccga ccgaggggca cctcctcgtg ctcccacggg gcgggatcgc
     7081 tcgggggcac gtcctcgacc cccagctcga acgccgggaa cggccgtccc cagtgccctt
     7141 cgaaccgggt tgccgcgtcc atgaccaact cgtcgaaccg ctccgcgcgg ctgcgcatcg
     7201 ccggaaccgg cggccacgcc agcggacccc gcaccccgcg gccatggcgg cggcgggtgc
     7261 gcggcgcgcg gggatcgtca cggttcacgc gctccactct acggcgcggg tatgccgtgt
     7321 gcccgggtcc gcgggccggc tgcggcgcgc cgaggcggca tacacccggc ccgggtttag
     7381 agtgcccgcg tgggcttgac tcggcagtgt tccaagacgg ggtgtcggcg gtcggccgtt
     7441 gccaccctga cctacgtcta tgccgaccag accgccgtgc tcggcccctt ggccacctat
     7501 gccgaaccgc acacctacga cctgtgcggg cagcacgccg aacggctgac cgtcccgcgg
     7561 gggtgggatg tggtccgtct ggccttcgac ccgagcccgc cggcaccctc atccgacgac
     7621 ctgctcgccc tcgccgacgc ggtccgcgag gccggacggc cccgctcgcg cgccccattg
     7681 agcccccctt cgagcccctc gcagtcaagc gccccaggcg cggccgaggc ctccccggcg
     7741 accggcgcac gccggggtca cctgagggcc ctgccgaaca actgacccct ggccaccgcg
     7801 ctaggctgcc gttcgtgacg agccgtgaca tccctgacct gacccagttc gtcaaggcat
     7861 acgacgtgcg cggcgtggtc ccggcccaac tcaacaccac cgtcgcccgt gccctcgggc
     7921 gcgccttcgc cgagatcgtc gcgctccccg acggggccgc aacggtggtg gtcggttacg
     7981 acatgcgacc cagctcgccg gagttgtcgg ctgcgttcgc cgacggtgtc gccgagaccg
     8041 ggatcgacgt catcatcatc ggcctcgcct cgaccgacca gctctattac gccagtggcg
     8101 ccctcgacct gccgggcgcg atgttcaccg cgagccacaa cccggcgcag tacaacggca
     8161 tcaagatgtg tcgggccggg gctcgccccg tcggtcagga caccgggttg gcccaggtcc
     8221 gcgacctggc ccagacactg ctcaacaacg ctgccaccga tggtgccgcc gtcgcggcca
     8281 ccgacgcggc ggggcgcctc ggagcggtca ctcaccggga tgtgttggcg gactacgccg
     8341 ccttcctgcg gtcgctggtc gacctgtccg gcaaccgccg gttgcgggtc gtcgtcgacg
     8401 cgggcaacgg catggggggc cacaccgtgc ccgcggtcct cgagacgggc gccgggctgg
     8461 cggaactgcc cctggaggtc attccgctct acttcgagtt ggacggcacc ttccccaacc
     8521 acgaggccaa ccccctcgag ccggagaacc tgcgtgacct ccagcgtgcc gtcgtcgagc
     8581 agggtgccga catcggccta gccttcgacg gtgacgccga tcggtgcttc gtcgtcgatg
     8641 agcgcggtga gccggtgagc cccagcgcca tcacggcgct cgtggccgct cgcgagatcg
     8701 cccgggccgt cgcaggcggc gacgatgtga gccaggtccg gatcgtccac aacgtcatca
     8761 cgtcactcgc cgtgcccgag atcgtcaccg agcttggggc cgtgcccgtc cgcacccgcg
     8821 tgggtcactc cttcatcaag gccgaaatgg cctcggctgc agcggttttc ggcggcgaac
     8881 actcggctca ctactacttc cgcgacttct ggttcgccga cacgggcatg ctcgccgcgc
     8941 tgcatgtctt ggccgccctc ggggagtcgg acgcaccgct gtcggtgctt ggggcggcat
     9001 acgagaggta tgccgcgtcc ggcgagatca actcgacggt cgccgaccgg gaggccgcga
     9061 cgacgcgtgc gctggactgg gcgcgcgcgc aggggccggt cgagatcgac accctcgacg
     9121 gcaccacggt gcgccacctc ggggagccga tgtggtggtt caacgtccgg gcctccaaca
     9181 ccgagccgtt gctgcgcctc aacgtcgagg cggccgacgc cgccacgatg gcttcgatcc
     9241 gcgactgcgt cctcggcctg ctgcgccagt cggcgccagc agtgccggac gctgcggcgg
     9301 ccccggcaca gccgtctgag gaggcggccc acctggagcc ttggctgcgc gagatcctgc
     9361 gctgcccggc ctgtcgcaag ccgctgcgcg acgagaccgg cccagccggt cctgagttgg
     9421 tctgcacctc acccgagtgc gggttggcct atccgatcga gggcgggatc cccgtcctgc
     9481 ttgccgatct ggcccgcaag ccggtctgag cagacgcccg atctcggtcg ggttcgcacc
     9541 acaaggagga gccggtatgc cgcgctggga cgacagactc gtcgacgacg aggccgagcg
     9601 cgagcggctg gacagccggg gaacgctgcg agcgctggcc accgccggag cccaggtccg
     9661 ccaatcgctc gggttggccc aagaggccgg catcgcccgc gtcgccggag gggttcgtcc
     9721 gcgcggggtg ctcgttgccg cgcggggtgg cacgggggtc atcggtgaca ccttgcagat
     9781 ggtggccgag ccggggtctc cggtgccgat cagcacggca gggacaccgc cccttccggg
     9841 gtgggtgggg accctcgacc tggtcattgc ggtgtcggag tcgggtcggg ccgccggacc
     9901 gctggccctg gctgcggcgg ctgcccgacg cggggcatcg ctgctcaccg tgggcgctga
     9961 tgactcgccg ttggccgccg tctgcgcctc ggcccgcggc atccatgtcg ggctgcctgc
    10021 cggtgcgacg tccagccgca cggccctctg ggccctgctg acgccggtcc tgctggctgc
    10081 tgacgtgttg ggcctggcca ggaccggacc cgacctgctc gaagccgtgg ctgaccgcct
    10141 cgatgccggt gcggcggcat accggccgtc ctcggaggcg ttcgtcaacc cggccaagct
    10201 cctggcggcc gacctgtccg ggggacttcc cgtggtgctc ggggacggcg cggtggcggg
    10261 ggtcgccgcc cgccgggccg cgatgatgct tgcccggacc gctcgcgtgc cggcgacggt
    10321 cggcgagttg cccggcggcg cttccgaggt cgtggcgctc ctcgacgggc cctatacgcg
    10381 ccacggagcc cgcgagcccg acctggcctc ggccgtcgac gacatcttcc gcgacccctt
    10441 cctggacggc gcccccgatc cgcgactggt cctgctcatg ttgcggggag cgctggctgc
    10501 cgggatgacg cccgcaggcg tcggcggctc gatgcccgaa gacaccgacg acctcggcga
    10561 tgcggtcgcg acggcagcaa tggacgccgg agtgcgggtt cacgagcgcc gggccgagcc
    10621 gggccacccg ttggaacaac tggccgacct catcggcctg gtcgatttcg cggcgaccta
    10681 tctcgcgctg ggcctgggcc tggatccagg ggtttcggcg cacgtccggg cgctgcgcga
    10741 ccacggccgc tgaccctcag aacgcggatg ccgggtgccc gccgcgacgg cgagcacccg
    10801 gcataccgga gaaatgcgtc agtctgtcgc ggctcagtcc tcggggacca tgtggatcga
    10861 cccgcacggg cactccgcga cgcacatgcc gcagcccttg cagtagtcgt agtcgatctc
    10921 gtagccgtgg ccgatgctgg tcttcttgac cgcgttgtcc gggcagacac cgaagcagtt
    10981 gtcgcaggcg aagcagttgc cgcacgacat gcaccggcgc gcttcgaact gcgcggtctc
    11041 ctcatcgagc ccggtcacga cctcgtcgaa ggtcgacgcc cgacgggcac cttcgaggtg
    11101 ctcgcggtgc tggtgggggg catcggcgta gtaccaggtg gtgagcttct cgaaggtcgc
    11161 caccgcgtgc ttggccggcg gggtgtatgc cgtgccgcgc aggtagccgt cgatgtagcg
    11221 agccgccttc ttgccgtgtc cgacggcgac ggtcaccgtg cgctcggccg gaaccatgtc
    11281 accaccggcg aagatgcccg cgcggccggt catcatcgtg ccgtcgaccg cgaccacacc
    11341 gtcggcgacg gtgatgccgg caacgccctc aaggaagctc aggtcggcct cttgccccag
    11401 agcaaggatg agcgagtcgg cttccaactc ctcgaattcg cctgtgggct gcgggaatcc
    11461 ggtctcgtcg agagccatct tctcgacgac catcttgcct tcgtcgacgt gcttgatcgt
    11521 cgagagccac ttgagcagga tgccttcctc ctgcgcctcc tggacctcga agtcgtgcgc
    11581 cggcatcttg tcgcggttgc ggcggtagac gatgatcgcc tcttccgcgc cgagccgctt
    11641 ggccgtgcgc gcggcgtcga tggccgtgtt gccgccgccg tagacgacga cccggcgccc
    11701 cagcatgggc ttctcttcct gggtctccac gtcgtgcagg tagctcaccg cgtcgaggat
    11761 cttggccgac gagccggccg ggatgtaggc ccgcttgccg atgtgcgcgc cgacggcgag
    11821 gaagaccgcc tcgaagccct cggccagcgc accctccagg tcactcacct tggtgttgag
    11881 ctcgagggtg accccgatgt cggtgatccg cttgacctcg gcgtcgagga tgttgcgcgg
    11941 caaccggtag gaggggatgc caaagcgcat catcccgccg gccatcgggc ccgcgtcgcg
    12001 caccgtcacc tcgtggccga gtcggcgcag gtggtatgcc gccgacagcc ctgaggggcc
    12061 ggcaccgatg acgaggaccc gcttgcccgt gagcagttcg gcggctggag gctcgaacgc
    12121 ccaacccagc tcgagggcct ggtcgccgag gaagcgctcg acggcgttga tgccgaccgc
    12181 ttcgtcgacc tgggcccggt tacaggcggt ctcgcacggg tggtagcaga cccggcccat
    12241 gatcgccggg aacgggttgt cttgggtcag ttgccgccag gccgcctcgt agttgccctc
    12301 ctcagcgtgg aacagccatt gctggatgtt ctcgccggcg gggcagccct ggttgcacgg
    12361 gggcaacagg tcgacgtaga gaggcttctc ggtgcgccag gacccggtgt ggttggccag
    12421 gctcgaaccg aggtcgaggg tgatcgcaaa gggcttgtcc atcagtcctg ggctttcgtc
    12481 tcggtggcgc cggccgtcgg cagcaggttg taacggcgga tgttgcggtc ggcctgggcc
    12541 tgcattcggt cgatgacgtc ctgccggcgg gtcggtgaga acaggtgggc gaagcgcttc
    12601 tggcccttga ggtagtcctc gaccggcacc tgacggcgga tgggggagga cgcgacgacc
    12661 tcgccgtact cagcctcgaa gaccgggaag atgccgctct gggtcgccag ccgggccacc
    12721 ttgatcgtgt cgccggaggc ggtgccccag ccgagcgggc acgggaccat gacgtggatg
    12781 tagcgcgcgc cgcgcatcga catggcccgg tggaccttgg cctccaggtc gcgcaggtcg
    12841 gcgaccgatg ccgttgcgac gtaagggatc tcgtgtgcca tggcgatccg ggggaggtcc
    12901 ttgccctgcc cgaaggtgtt gcctgggtcc tcgccgacgg cctgcgtggt ggcggtccgc
    12961 gctgccgggg gagtggcccc ggagcgctgg atgccggtgt tcatgtaggc ctcgttgtcg
    13021 taacagatgt agagcacgtc gtcgttgcgc tcgaacatcc ctgacagaca gccgaacccg
    13081 atgtcgacgg tgccgccgtc gccgccctgg gcgaccaccc gcacgtcgct gcgtcccatg
    13141 gccttgtatg ccgccgcgac gccggatgcg acggccggag cgttgccgaa cagcgagtgc
    13201 agccaggcca cgcgccacga ggtctcgggg tagggcgtgg agaacacctc aaggcagccg
    13261 gtggcgttga cggcgacgat cttgccgtcc gccgcgtgca tcgccgcgtc gagggcgtag
    13321 cgggcgccga gcgcctcgcc gcatccttgg caggcccggt ggccggaggt gagggagttg
    13381 gtgcggttgg ggtcggactg gaccgagcgg acctcca
//
LOCUS       DAORPW010000056        13350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_954_length_13350_cov_706.214366, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000056 DAORPW010000000
VERSION     DAORPW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13350
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_954_length_13350_cov_706.214366"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLB86_06425"
     CDS             complement(<1..377)
                     /locus_tag="PLB86_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64806.1"
                     /translation="MGPGWGARVAGAFTAPIPPEEAVPRTADAPGTPWWRRFPLWFPL
                     AVYAVTRLIDAVYFAIMQRSQIPMVPGNGAARVIFPTPAAPGYWPLMANWDGQWYHEI
                     AELGYPDPIPTDPVTGKVVQNPWA"
     gene            complement(433..2034)
                     /locus_tag="PLB86_06430"
     CDS             complement(433..2034)
                     /locus_tag="PLB86_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRC64807.1"
                     /translation="MPPSTDPTEAPFAGPSASEGGDPRVDSVRERFAAAYLATSAPLT
                     ESLGAKLVQQNKLYVRDRIALLFDDGSFVEDGRYANALAGDLPADGVVTGRGTVDGRP
                     AIVIANDPTVKAGSWGARTVEKIVRATEMAMREELPVFWFVDSAGARITDQVEMFPGR
                     RGAGRIFHNQVALSGKVPQICCLFGPSAAGGAYIPSFCDLIIMVEGNASMYLGSPRMA
                     EMVVGEKVTLEEMGGARMHCTVSGVGDLLAHDDQEAIELAKLYFSYVPGCWRERPPTY
                     TPEEPSEPLTRHTVPERESQPFDIHTVIDGLVDADSFYPIKPLFAAELVVGFGRMNGE
                     TVGIVANNSAVKGGVLFTDSADKATRFIWLCDAFNVPLLYLADVPGFMIGSEVERGGI
                     IRHGAKMVSAVSEATVPQFCIVVRKAYGAGLYAMGGPGFAPDATLALPTAKIAVMGPE
                     AAVNAVYANKIASIADPDERAAYIAERRAEYEEDVDLERLAADLVVDGVIEADALREE
                     LLHRLRYASRRDRHFSERRRSVPPV"
     gene            complement(2087..3292)
                     /locus_tag="PLB86_06435"
     CDS             complement(2087..3292)
                     /locus_tag="PLB86_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC64808.1"
                     /translation="MFELSADHEEFRALVREFAEAEIEPRVAQWDKDSHFPAELIPKL
                     GELGLFGLVVPEEFGGTRRPEGAGPEVSGGDFTMLCVAIEELGRVDQAIGITLEAGVG
                     LGINPILNYGTPEQKAHWLPDLAAGRALAGFGLTEPDAGSDAGGTKTKATLADGIWTV
                     NGAKSFITNSGSPITSVVSVTARTGTRNDGTPEISAIMIPSGTKGFDIAPPYHKLGWH
                     ISDTHGLSFQDVQVPEANLLGQRGKGFAQFLATLDDGRIAIAALATGCTQRMLEEATA
                     YSKTRLAFGKPIGTYQGVAFQVSDLAVLAETSRLLTYKAAWLKDEMDAGRRTMQEVKQ
                     AASIAKLYTSEAAVTATRIATQVFGGNGFMEEYPVARFYRDAKILEIGEGTSEVQRML
                     IARGLGLPA"
     gene            3412..3807
                     /locus_tag="PLB86_06440"
     CDS             3412..3807
                     /locus_tag="PLB86_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRC64809.1"
                     /translation="MASGRDGERTWSIAQVAAEFGVTHRTVRHYEDIGLIAPERHGTR
                     RVFRRRDRTRLALILRGKRLGFPLEEIRTIIDLYDAPRGKTSQLEYAITQARKRRVDL
                     EERRRDIDESLAELGAFEASCQADLDRLS"
     gene            3931..4407
                     /locus_tag="PLB86_06445"
     CDS             3931..4407
                     /locus_tag="PLB86_06445"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRC64810.1"
                     /translation="MGFFNNAKLEPTPELAPLTKERIKATLEASSWSYHVDSDGDIGS
                     GWDHGVIWFFTYGNQAEILQVRGIWDGKLSPDDLPQALDVVNEWNCDRLFPKVYARIN
                     DEGQLRVISEHTLDYEHGLTDEQLNLHVNTGIATSCQLWERLNEAFPEAAAAAAAE"
     gene            4451..6271
                     /locus_tag="PLB86_06450"
     CDS             4451..6271
                     /locus_tag="PLB86_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HSP90 family protein"
                     /protein_id="HRC64811.1"
                     /translation="MSQRFRVDLGGMVDLLSHHLYSGPHVYLRELLQNAVDAVTARLA
                     EDPAAPATIRLTSDTEADGATVLVVTDSGVGLTSDEATELLATIGRSSKRDPHGLGVA
                     HYLGQFGIGMLAAFMVADRIEVTSRSARPGTVPTVWVGRSDGTFEVSDLPPGPSADAT
                     AVGTTVRLWARRGSEHWLETSTVVALATDYGSLLPFDVAVRVPVEGSDPTWRRITDPV
                     LPWQARHPSAEARSRALTAYCERVLGFTPLGHIDLSVPLAGVSGVAFILPQAVTPGSG
                     RHRVYSKRMLVGARVDGVLPDWAFFTRAVLDSDALSPTASREQIHDDEILLATRDALG
                     AQLKEWATATLTTPSTLSRRFVETHHLALRSLALTDDEMLDLAARVLPFETTDGARTL
                     RDVAEDGEIVFTSTTEAFRRVAAVARAQGLVVVNAGYVYDSDLLTRLGRRPGWAVREL
                     SSEDVTQVLTPVATDRAGQLAQALSMAEKVLEDEDCHVLVRTFDPDSLPAVLLRDPDG
                     EHRRALAREREATPGLWGGLLDSFETDTSTRTRSLVLNDSAPVVRQLLAAPETIVFAA
                     GLRSLYLSAVMLAGEGLRSSEVAGLHDALSVLLARSLDHD"
     assembly_gap    6275..6374
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6435..8399
                     /locus_tag="PLB86_06455"
     CDS             6435..8399
                     /locus_tag="PLB86_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64812.1"
                     /translation="MAPSDAEVRRELREIRAMPYGSARTAAAEVIVRRIDAEGPQERL
                     AEALLDLVEAYSFTGQGHRSFVAFARLLRLWDSQPEVFDESDEHNLFWEFKWVAGDLP
                     DFPEITREQADAFLADMRRRFDLAGKGSGSVLGSRFRWAWHSGDPSMEAARLAWLALP
                     KDDMDSCAACRIGHQVDYLTETGRFEEAIELGLTQHSSCNLEPTRTLHALALAHLNVG
                     ADGPALAAYRRALATLDTSDSDYAPARGQGFEMVARGGHVERALRFLREDHPELLEGA
                     STPLFRLRFLLGVLAGLSANLDHSDLPTGLRSPDAPTLGQLHAWVHEQTAELAGRFDA
                     RGGSDYYARLLDRSLAATRTPVPLDFDISAMASLGPQPGAGDPTHQPDADPAPAALGA
                     DGVAAALTIAERHARARSYPEAAAAYLRAAEVATAAGLLEHAGMAWAESAQCAAQVGD
                     EVTAHARYAAALPLLRAADGDPGAMAQILAAWAPVAAGLGDTDALMTHLDTLLSSDGS
                     HEALLDSDLEERQRRQSENLQASLLETWARVVASRSPGVDGQIDGQVAGQSARDAADA
                     ATRAGELFAQVGRIGSAAQAFWLAGQILREDGDLDGAVLALESAYEGLTIARDAANRA
                     RAASDLIDVLRATGQHDRADQVIATLTSGS"
     gene            8520..10313
                     /locus_tag="PLB86_06460"
     CDS             8520..10313
                     /locus_tag="PLB86_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRC64813.1"
                     /translation="MAKISKVLIANRGEIAVRIARACKDAGIGSVAVYADPDRDALHV
                     KVADEAYALNGSTPGDSYLVQEKLLAIAKEAGADAVHPGYGFLAENAGFAQAVIDAGL
                     IWIGPGPAAIDSLGDKVKARHIALKANAPLVPGTSDPVTGPNEVVEFAKAHGLPVAIK
                     AAYGGGGRGLKVARELEEIPHLYESAVREAVTAFGRGECFVERFLDHPRHVETQCLAD
                     QHGNVVVVSTRDCSLQRRNQKLVEEAPAPFLTEAQHDELVRASKAILTEAGYVGAGTC
                     EFLVGPAGDISFLEVNTRLQVEHPVTEEVTGIDLVREQFRIAEGQSLDYPDPVPHAHS
                     IEFRINGEDAGRGFLPAPGTVTVYKTPSGPGVRVDSGLVEGDTIGGAFDSMLAKLIVT
                     GRDRQQALERSRRALAEFEVEGMPTVIPFHRAVVADPAFAPADPHEAFSIHTRWIETE
                     FNNTIPAYAGGPASLPEAEERQKLVVEVGGKRIEVSLPGGLSLGGGTGGSGSGATRKA
                     APKRSGAAKSGAAASGDSVTAPMQGTIVKVAVEEGQTVESGAIVVVLEAMKMEQPITA
                     HKAGVITSLKATVGETVASGTVLAEIKDAEG"
     gene            10313..10507
                     /locus_tag="PLB86_06465"
     CDS             10313..10507
                     /locus_tag="PLB86_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64814.1"
                     /translation="MPTEGFSFVNLAILAVVALIVVPGYRHLRGTVSARRRERWAQEE
                     ADYRAAVENQDPGQDHPAGA"
     gene            complement(10523..12517)
                     /locus_tag="PLB86_06470"
     CDS             complement(10523..12517)
                     /locus_tag="PLB86_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64815.1"
                     /translation="MSWPQLLPAVLVAVAVFFVPGAVLLRSVGIGWLTAVLAAPAVSV
                     AVIIAAGLGVRAVGVGWSAVTGLIAVVLVVALGLVAARAVRSRWPGSVPRSSDAAPLR
                     AVLAVGAVSLAAAYLVLAGGAASPMAIPQMPDVIFHLGAVEWMVRHGSASALDTYQYA
                     QLDSPLTYPGAFHAVTAATVAWTGLPVTATWHALLIVVVGLVWPFGVMLLARVVLGRG
                     TGILVAAGLLTLVFTSFPTRFLAWGPLWSNLLANALLPAVLAGALCAVAPSAMLAERA
                     FASGRVRAVGYAALGLLVLLGTQPNAVASGVVIGLTVVAAALPQWRHALGGRWGARFV
                     RLPWFLLMVAAAVMASTRFIPTKMYVINSHVRATWALAGSQAATLFDGSWLESGSVVV
                     LVLVGAVWLARDRNRRWLVVSLALFIAIFLLLYAVNGPVVRQLTWLWWNDHYRVRAAL
                     VLPAVLCATAGAVVIGGWVARRRPDPPRVRQGLIALAAVAVVAALGCGIPVHREVVEA
                     TYEEDSADYSWVLPREYAALSQLAAFVPSGSVVAANPYRGGMFFYVAGGTEVLYPTEN
                     SLQLPDRRLLGTSLQAVTSMPDVCAASRRQRVDYVLTGGDMHIWGVLDHTPEYSGVDS
                     AAYDPDFKVVAEAGPYALRRVPDCSTAAPGSGFIGPVTIP"
     gene            complement(12535..>13350)
                     /locus_tag="PLB86_06475"
     CDS             complement(12535..>13350)
                     /locus_tag="PLB86_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC64816.1"
                     /translation="YLGLGSAVTLVSSRHQVLPGEDSDAAAVIEEVFRREGMEVLGRS
                     RMSSVERTADGVIVRLEDGRTVEGSHALLAVGSVPQTAGLGLEDVGVEVGPGGHVLVD
                     GVSRTSVRGVYAAGDCTGVLPLASVAAMQGRIAMWHALGDAVAPLRLDVVSANVFTDP
                     EIATVGVSQQDVDAGTVDAIVITLPLTTNARAKMDGFHDGFVKIFARSGHGTVLGGVV
                     VAPRASELIYPLTIAVENGLTADQLSNAFTVYPSMTGSIGEAARRLHVAARMS"
BASE COUNT         2131 a         4672 c         4585 g         1862 t
ORIGIN      
        1 gcccacgggt tctgcacgac cttgccggtg accggatcgg tggggatcgg gtcgggatac
       61 cccaactcgg cgatctcgtg gtaccactgc ccgtcccagt tggccatgag cggccagtac
      121 cccggcgcgg caggcgtggg gaagatgacc cgcgcggcgc cgttgcccgg caccatcggg
      181 atctgcgagc gctgcatgat cgcgaagtag acggcgtcga tgagccgggt cacggcatac
      241 accgcgagtg ggaaccacag ggggaagcgg cgccaccacg gcgtcccggg agcgtccgcg
      301 gtgcgcggca cggcctcctc aggcgggatc ggtgccgtga aggcgccagc cactcgcgcg
      361 ccccaccctg gtcccatggt gggcaatggt agaggggtat gccgccgccg gtcaccgccc
      421 gggcgaccga cctcagacag gcgggacgga acggcggcgt tcgctgaagt gccgatcccg
      481 tcgggaggcg tagcgcagcc ggtggagcaa ctcctcgcgc agggcatcgg cctcgatgac
      541 cccgtcgacg acgaggtcgg cggccaggcg ttcgaggtcg acgtcctcct cgtattcggc
      601 ccgtcgctcc gcgatgtagg cggcccgctc gtcggggtcg gcgatgctgg cgatcttgtt
      661 ggcgtagacg gcgttgacgg ccgcctccgg ccccatgacg gcgatcttgg ctgtgggcag
      721 ggcaagggtg gcatcggggg cgaaaccagg cccgcccatg gcgtaaagcc ctgcgccata
      781 agcctttcgg acgacgatgc agaactgcgg cactgtcgcc tcggagacgg ccgacaccat
      841 cttggcgcca tgacggatga tcccgccgcg ctcgacctcg gagccgatca tgaagcctgg
      901 gacgtcggcg agatagagga gcggcacgtt gaaggcgtcg cacaaccaga tgaaccgggt
      961 ggccttgtcg gcgctgtcgg tgaacagcac gccgcccttg acggcggagt tgttggccac
     1021 gatgccgacg gtctcgccgt tcatccgacc gaagccgacc accagctcgg cagcgaagag
     1081 cggcttgatc gggtagaacg agtcggcgtc gacgagcccg tcgatgaccg tgtggatgtc
     1141 gaacggctgg ctctcccgct caggcacggt atgccgcgtg agcggctccg agggctcctc
     1201 cggggtgtag gtgggcgggc gttcgcgcca acagccgggg acgtaggaga agtagagctt
     1261 ggccagctcg atggcctcct ggtcgtcgtg tgccagcaga tcgcccacgc ccgagacggt
     1321 gcagtgcatc cgcgcgccgc ccatctcctc gagggtgacc ttctcaccga cgaccatctc
     1381 ggccatccga gggctgccga gatacatcga ggcgttgccc tcgaccatga tgatgaggtc
     1441 gcagaagctc gggatgtagg ccccgccggc cgccgaggga ccgaacaagc agcagatctg
     1501 gggcaccttg ccggacagcg ccacctggtt gtggaagatc cggccggcgc cgcgccgccc
     1561 ggggaacatc tcgacctggt cggtgatccg ggcaccggcc gagtcgacga accagaagac
     1621 cggcaactcc tcgcgcatcg ccatctcggt ggcgcggacg atcttctcga cggtccgggc
     1681 tccccacgag ccggccttga cggtcgggtc gttggcgatg acgatcgcgg ggcgcccgtc
     1741 gaccgtcccc cgaccagtga cgacgccgtc ggcaggtagg tcgccggcga gggcgttggc
     1801 atagcgcccg tcctcgacga aggacccgtc atcgaacagc agcgcgatcc ggtcgcggac
     1861 gtagagcttg ttctgttgta cgagcttggc cccgaggctc tcggtgaggg gggcggaggt
     1921 cgccaggtat gccgccgcga accgctcccg gacgctgtcg acccgagggt cgccaccctc
     1981 ggacgcggac ggaccggcga agggggcttc tgtcgggtcg gtgctcggcg gcatacctca
     2041 tcctctccca gggggaacgc gtcggtgacg tgacgtgcgc tgatcctcag gcgggcagac
     2101 cgaggcctcg ggcgatgagc atccgctgga cctccgaggt gccctcgccg atctcgagga
     2161 tcttggcgtc gcgatagaac cgcgccaccg ggtattcctc catgaagccg ttgccgccga
     2221 acacctgtgt ggcgatccga gtcgcggtga ccgccgcctc gctggtgtag agcttcgcga
     2281 tggacgcggc ctgcttgacc tcttgcatgg tgcgtcgccc ggcgtccatc tcgtctttga
     2341 gccaggccgc cttgtaggtc agcaggcggc tcgtctcggc caggaccgcg aggtcggaga
     2401 cctggaaggc cacgccctgg taggtcccga tcggcttgcc gaaggccaac cgcgtcttgc
     2461 tgtatgccgt ggcctcctca agcatccgct gcgtgcaacc cgtcgcgagg gcggcgatgg
     2521 caatccgtcc gtcatcgagg gtggcgagga actgcgcgaa gcccttgccc cgctgaccga
     2581 gcaggttggc ctcgggcacc tgcacgtcct ggaaggacag gccgtgggtg tcggagatgt
     2641 gccacccaag cttgtggtag ggcggagcga tatcgaatcc ctttgtgccg gaagggatca
     2701 tgatcgcgct gatctcgggc gtcccgtcgt tgcgggtgcc ggtgcgggcc gtgacgctga
     2761 cgaccgaagt gatcggcgag ccggagttgg tgatgaacga cttggccccg ttaaccgtcc
     2821 agatgccgtc ggcgagggtc gccttggtct tcgtgccgcc cgcgtccgag ccggcgtccg
     2881 gctcggtgag gccgaacccg gcgagggccc gtccggcggc aaggtcgggc aaccagtggg
     2941 ccttctgctc aggggtgccg tagttgagga tcgggttgat gccgagcccg accccggctt
     3001 cgagggtgat gccgatggct tgatcgaccc ggccgagttc ctcgatcgcg acgcacagca
     3061 tggtgaagtc gccgccgctg acctcggggc cggcgccctc ggggcggcgg gtgccgccga
     3121 actcctccgg caccaccagg ccgaacaggc cgagctcacc gagtttgggg atgagctcag
     3181 cggggaagtg cgagtccttg tcccactggg cgacccgggg ctcgatctcg gcctcggcga
     3241 actcccgcac tagagcccgg aactcctcgt ggtcggcgct cagctcgaac atggctgcct
     3301 cacttccgtg ccgcaacccg cagcgtcggt gacgggcgtg gcgttggcgg ggacgcggca
     3361 taccgccatc cctagttgac gttaacgtca acgtaaagca tgctgacccc catggcaagc
     3421 ggacgggacg gcgagcggac gtggtctatc gcacaggtgg cggccgagtt cggggtcacc
     3481 catcggacgg tgcggcacta cgaggacatc ggcctcatcg cccccgagcg acatggcacc
     3541 cggcgggtct tccgccgccg cgaccggacc cggctggcgc tcatcctgcg cggcaagcgg
     3601 ctgggcttcc cgctcgagga gattcgcacg atcatcgacc tctacgatgc gccgcgcggc
     3661 aagaccagcc agttggaata cgccatcacc caggcgcgca aacggcgggt cgaccttgag
     3721 gaacgccgtc gggacatcga cgaatcgcta gccgaattgg gcgcgttcga ggcgtcgtgc
     3781 caggccgatc tcgaccggct cagctgaagg ccagcgaggg cggcgccgcg gctgccgcac
     3841 ccggttgaca accggaagga gcgcgcggaa gggtggtctc tccggcgggg catccgcgcg
     3901 gcacactcgg gggcaggaaa gggcacatcg atgggcttct tcaacaacgc caaacttgaa
     3961 ccgacaccag aactggcgcc attgaccaag gagcgcatca aagcaaccct cgaagccagc
     4021 agttggtcgt atcacgtcga ctccgacggt gacatcggca gcggctggga tcacggggtc
     4081 atttggttct tcacctacgg caaccaggcc gagatcctgc aggttcgagg gatctgggac
     4141 ggcaagctca gcccggacga cctgccgcag gccctcgacg tcgtcaacga atggaactgc
     4201 gaccggctgt tccccaaggt ctatgcccgg atcaacgacg agggccagct ccgggtgatc
     4261 tctgagcaca ccttggacta cgagcacggg ctgaccgacg agcagctcaa cctgcacgtc
     4321 aacacgggga tcgccacgag ctgtcagctc tgggaacggc tcaacgaggc cttccccgag
     4381 gcggctgcgg ctgccgccgc ggaatgacgc cgacggccgc ccgctccggc gctgcggcgc
     4441 acggagcctg atgtcgcagc gcttccgggt cgacctgggt ggcatggtcg acctgctgtc
     4501 ccaccacctc tactcgggtc cgcatgtcta cctgagggag ttgctgcaga acgccgtcga
     4561 cgccgtgacc gcgaggttgg ccgaggatcc cgcagccccg gcaacgatcc ggctcacctc
     4621 ggacaccgag gccgacggag cgacggttct cgtggtcacc gacagcgggg tcggactcac
     4681 ctccgacgaa gccaccgagt tgctcgcgac gatcggccgc tcctcgaaac gggatccgca
     4741 cggcctgggg gtggcgcact acctcggcca gttcgggatc ggcatgctcg cggcgttcat
     4801 ggtcgccgac cgcatcgagg tcacgtcccg gtcggcccga cccggcaccg tccccaccgt
     4861 ctgggtgggg cgcagcgacg gcaccttcga ggtgtcggac ctgccgccgg ggccgtccgc
     4921 ggacgcgacg gcagtgggca ccaccgtccg gctgtgggct cgacgcggca gcgagcattg
     4981 gctcgaaacc tccaccgtcg tcgctctggc cacggactac ggctcgctgc tgcccttcga
     5041 tgtcgccgtc cgcgtgccgg tggagggctc ggatcccacg tggcggcgga tcaccgaccc
     5101 ggtcctcccg tggcaggccc gccacccgtc ggcggaggcc cgctcgcggg cgctgacggc
     5161 atactgcgaa cgcgtcctgg gcttcacccc gctgggacac atcgacctga gcgtccccct
     5221 cgccggcgtg tccggggtgg cgttcatcct cccccaagcc gtgacgccgg gctcgggacg
     5281 gcaccgcgtc tacagcaagc ggatgctcgt cggggcgcgc gtcgacggcg tcctgcccga
     5341 ctgggccttc ttcacccggg cggtcctcga ctcggacgcg ctctccccca ccgcgtcccg
     5401 cgaacagatc cacgacgacg agatcctcct ggcgacccgt gacgccttgg gggcgcaact
     5461 caaggagtgg gccaccgcca ctctgacgac gccctcgacc ctgtcacggc ggttcgtcga
     5521 gacccaccat ctggccctgc gctcgctcgc cctgaccgac gacgagatgc tcgacctcgc
     5581 cgcccgggtc ctgccgttcg agaccaccga tggcgcacga accttgcgcg acgtcgcgga
     5641 ggacggcgag atcgtgttca cgtcaacgac cgaggcgttc cgacgggtcg ccgcggtcgc
     5701 ccgcgcccag ggcctggtcg tcgtcaacgc gggctacgtc tacgacagcg acctgctcac
     5761 ccgactcggg cggcgcccag gctgggcggt ccgcgagttg tcgagcgagg acgtcaccca
     5821 ggtcctgaca ccggtggcaa ccgaccgcgc cgggcagctc gcccaggccc tgtcgatggc
     5881 tgaaaaggtc ctcgaggacg aggactgtca cgtcctcgtg cgcacgttcg accccgattc
     5941 gctgcccgcc gtgctgctgc gcgaccctga cggcgagcat cgacgggccc tggcccgtga
     6001 gcgggaggcc acgccgggcc tctggggcgg gctcctcgac tccttcgaga ccgacacctc
     6061 gacgcggacg cgcagcctcg tgctcaacga ctcggccccc gtcgtgcggc aactgcttgc
     6121 ggcaccagag accatcgtgt tcgccgcggg actgcgctcg ctctatctct cggccgtcat
     6181 gctcgccggc gaaggtcttc gatcgagcga ggtggccggc ctgcacgacg cgctgagcgt
     6241 gctgctggcc cgatccctcg accacgactg acctnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnngcactg accacgtctg ccctgaccac gtctgcactg accacgcccg
     6421 agaggacacc tcagatggca ccaagcgacg ccgaggtccg gcgcgaactc cgcgagatcc
     6481 gggccatgcc ctacggcagc gcccgcaccg ccgccgcgga ggtcatcgtc cggcgcatcg
     6541 atgccgaagg ccctcaggag cgtctggccg aggcgttgct ggatctcgtc gaggcctaca
     6601 gcttcaccgg tcaggggcac cgatcgttcg tcgccttcgc ccggctgttg cggctctggg
     6661 acagccaacc cgaagtgttc gacgagtccg acgagcacaa cctgttctgg gagttcaaat
     6721 gggtcgccgg cgatctgccc gacttccccg agatcacccg cgagcaggcc gacgccttcc
     6781 tcgccgacat gagacgccgc ttcgacctcg ccggcaaggg cagcgggtcg gtgctggggt
     6841 cgcggttccg ctgggcctgg cacagtggcg acccgagcat ggaggcggcg cggctggcgt
     6901 ggctggcgct gcccaaggac gacatggaca gttgcgccgc gtgccggatc gggcaccagg
     6961 tcgactacct gaccgagacc gggcgcttcg aggaggcgat cgagctgggc ctgacccagc
     7021 actcgagctg caacctggag cccacccgaa ccctgcacgc cctggccctg gcccacctca
     7081 acgtcggcgc ggacgggccg gcgctcgcgg cataccgtag ggccctggcc acgctggaca
     7141 cctccgacag cgactacgcg ccggcccgcg gacagggctt cgagatggtg gcgcggggcg
     7201 gtcacgtcga gcgggccctg cgcttcctgc gtgaggacca ccccgagttg ctcgaaggcg
     7261 catcgacacc cttgttccgg ttgcggttcc tgttgggcgt gctcgccggg ctctcggcca
     7321 acctcgacca cagcgacctg ccgaccgggc tgcgctcccc tgacgccccg accctcgggc
     7381 agttgcacgc ttgggttcac gagcagaccg ctgagttggc cggtcgcttc gacgctcgcg
     7441 gcgggagcga ctactacgcc cggctgctgg accgttcgtt ggccgccacc cgcacgccgg
     7501 tgcccttgga cttcgacatc tcggccatgg cgtccctcgg cccgcagccg ggggccggcg
     7561 acccgacgca ccagcccgac gccgaccccg ccccggcagc cctcggggca gacggcgtgg
     7621 ccgccgcgct gacgatcgcc gagcgccacg cacgggcacg ctcctacccc gaggcggccg
     7681 cggcatacct gcgggccgcc gaggtggcca ctgcggcggg gctgctcgaa cacgcgggga
     7741 tggcgtgggc ggagtctgcg cagtgcgccg cccaggtggg tgacgaggtg accgcccatg
     7801 ccaggtatgc cgccgcactc cccctgctgc gcgcggccga cggcgacccc ggcgcgatgg
     7861 cgcagatcct cgctgcgtgg gcgcccgtgg ccgccgggct cggcgacacc gacgccctca
     7921 tgacccacct cgacaccctc ctgtcgtccg acggttccca cgaagccctc ctcgacagcg
     7981 acctggagga gcgtcagcgc cgtcagtccg agaacctcca ggcctcgctg ctggagacgt
     8041 gggcgcgcgt tgtcgcgtcg cgcagtccgg gggtcgacgg gcagatcgac gggcaggtcg
     8101 cggggcagtc ggcccgcgat gccgccgacg cggccacccg cgccggcgag ttgttcgccc
     8161 aggtcggccg gatcgggtcg gcagcacagg ccttctggct cgccggccag atcctgcgtg
     8221 aggacggcga cctcgacggg gcggtccttg cgctcgagtc ggcctacgag gggctcacga
     8281 tcgcccgcga cgccgccaac cgggcccggg cggccagcga cctcatcgac gtgttgcggg
     8341 ccactggaca gcacgatcgg gccgatcagg tgatcgccac gctgacgagc ggctcgtgac
     8401 ccggcccgct gtcaggccaa tcgccggctg tagggccaac ggacacccgg cgcaacgtga
     8461 gccatccctc cactgccgct ccggtggtgc ggcacgatcc gagcgctagg ctccccccca
     8521 tggcgaagat ctcaaaggtg ctgatcgcaa accgcggcga aatcgccgtg cgcatcgccc
     8581 gggcatgcaa ggacgctggc atcggatccg tggcggttta cgccgatccc gatcgcgacg
     8641 ccctgcacgt caaggtggcc gacgaggcgt atgcgctcaa cggctccaca cccggtgaca
     8701 gttacctcgt gcaggagaag ttgctggcca tcgccaagga ggccggggcc gacgcggtcc
     8761 accccggcta cggcttcctc gccgagaacg ccgggttcgc ccaggcggtc atcgacgccg
     8821 gcctgatctg gatcggcccc ggcccggcgg ccatcgacag cctcggcgac aaggtcaagg
     8881 cccggcacat cgccctcaag gccaacgccc ccctcgtgcc cggcacgtcc gacccggtga
     8941 ccgggcccaa cgaggtcgtg gagttcgcca aggcgcacgg gctgcccgtg gcgatcaagg
     9001 cggcctacgg cggcggcggg cgcggcctca aggtggcccg cgagcttgag gagatccccc
     9061 acctctacga atccgcggtt cgcgaggcgg tcaccgcgtt cggacgtggc gagtgcttcg
     9121 tcgagcgctt cctcgaccac ccgcgtcacg tcgaaaccca gtgtctggca gaccagcacg
     9181 gcaacgtggt cgtcgtgtcg acccgtgact gctcgctgca gcggcgcaac cagaagctcg
     9241 tcgaagaggc gcccgcgccg ttcctcaccg aggcccagca cgacgagctc gtccgagcct
     9301 ccaaggcgat cctcaccgag gccggctacg tcggcgcagg cacgtgcgag ttcctcgtcg
     9361 ggcccgccgg tgacatctcc ttcctcgaag tcaacacccg cctgcaggtc gagcacccgg
     9421 tcaccgaaga ggtgaccggc atcgacctgg tgcgtgagca gttccgcatc gccgagggcc
     9481 agtcgctgga ctaccccgac ccggtgccgc acgcccactc gatcgagttc cggatcaacg
     9541 gtgaggacgc cggtcgcggc ttcctcccgg cacccggcac cgtcacggtc tacaagaccc
     9601 cgtccggccc gggcgtccgc gtcgactccg ggctcgtcga aggcgacacc atcggtgggg
     9661 ccttcgactc gatgcttgcc aagctcatcg tcaccggccg cgaccgccag caggccctgg
     9721 agcggtcccg tcgcgccctg gccgagttcg aggtcgaggg gatgcccacg gtcatcccgt
     9781 tccaccgtgc ggtcgtcgcc gaccctgcct tcgccccggc cgaccctcac gaggcgttct
     9841 ccatccacac ccgctggatc gagaccgagt tcaacaacac catccctgcg tatgccggtg
     9901 ggccggcgtc gctgcccgag gccgaggagc gccagaagct cgtcgtcgag gtcggcggca
     9961 agcggatcga agtcagcctt ccgggaggcc tgtcgctcgg cggtggaacc ggcggcagtg
    10021 gcagcggagc gacgaggaag gcggcgccta agcgcagcgg cgcagccaag tccggcgccg
    10081 cagcatccgg tgactcggtg accgccccca tgcagggcac catcgtcaag gtcgcggtcg
    10141 aagaaggtca gacggtcgag tcgggcgcga tcgtcgtcgt cctcgaggcg atgaagatgg
    10201 agcagccgat caccgcacac aaggccgggg tcatcacctc gctcaaggcc accgtcggcg
    10261 agaccgtcgc cagcggcacg gtgctcgctg agatcaagga cgccgaaggc tgatgcccac
    10321 ggagggcttc tccttcgtca acctggccat cctcgcggtg gtcgcgctga tcgttgtgcc
    10381 cggctatcgg cacctgcgcg gcaccgtgtc tgcacgtcgt cgcgagcgct gggcgcagga
    10441 ggaggctgac taccgcgcag cggtcgagaa tcaggacccc ggccaggacc accctgcggg
    10501 tgcctgattc accgccgaag cctcagggga tggtcacagg gccgatgaaa ccgctgcccg
    10561 gggcggctgt cgaacaatcg ggcacccggc gcaatgcgta tggaccggcc tcagcaacga
    10621 ctttgaagtc tgggtcgtac gccgccgagt cgaccccgga gtactccggg gtgtggtcca
    10681 gcacccccca gatgtgcatg tctccacccg tcagcacgta gtcgaccctt tgacgacgtg
    10741 atgcggcaca gacgtcgggc atgcttgtga ctgcttggag cgacgttcca agcagtcgcc
    10801 gatcgggcag ttggagggag ttctcggtcg gatagagcac ctcggtgccc cccgcgacgt
    10861 agaagaacat cccgccgcga taggggttgg cggcgaccac cgaaccgctc gggacgaagg
    10921 ccgccagctg ggacagggcg gcatactccc gcgggagcac ccacgaatag tccgcggagt
    10981 cctcctcgta ggtggcctcc acgacctcac gatgcaccgg tatgccgcaa cccaatgcgg
    11041 cgacgactgc gacggcggcg agggcgatca gcccctgacg cacgcggggc ggatctggtc
    11101 gtcgtcgggc cacccatccg ccgatcacca ccgcaccggc cgtcgcgcag aggactgcgg
    11161 gcagcaccag ggcggcccgg acccggtagt ggtcgttcca ccagagccag gtgagctgtc
    11221 ggaccaccgg gccgttgacg gcgtacagca acaggaagat ggcgatgaaa agggcgagcg
    11281 agacgaccag ccagcggcgg ttccggtctc gggcaagcca gaccgcgccg acgaggacca
    11341 gcacgaccac ggagccggac tcgagccagg agccatcgaa gagggtcgcg gcctggctgc
    11401 ccgccaacgc ccacgtggca cgcacatgag agttgatgac atacatcttc gtcgggatga
    11461 accgggttga ggccatgacc gctgcggcaa ccatgaggag gaaccacggc agtcgcacga
    11521 atcgggcccc ccaacggccg cccaacgcat ggcgccactg cggaagcgct gccgcgacga
    11581 cggtgaggcc gatgacgacg cccgaggcga cggcgttggg ctgggtcccc agcagcacga
    11641 gaaggccaag ggcggcatac ccgacggcac ggacccgacc cgacgcgaag gcgcgctcgg
    11701 cgagcatcgc gctcggggcc accgcacaca aagcgccggc gaggacggcg ggcaggagcg
    11761 cgttggcgag caggttcgac cacaacggcc cccaggcgag gaagcgtgtc gggaacgacg
    11821 tgaagaccag cgtgagcagc ccagccgcga cgaggatgcc agtgccgcgg ccgagcacga
    11881 ctcgggccag gagcatgacg ccgaacggcc agacgaggcc gacgaccacg atgagcagcg
    11941 cgtgccaggt cgccgtgacg ggcaacccgg tccacgcgac cgtcgcggcg gtgaccgcgt
    12001 ggaaggcccc tgggtaggtc agcggactgt cgagttgggc gtactgatag gtgtcgagag
    12061 cggaggccga cccgtgccgc accatccatt cgaccgcgcc gaggtggaag atgacgtcgg
    12121 gcatctgcgg gatcgccatc ggcgaggctg ctcccccagc caggacgagg tatgccgccg
    12181 cgagactcac cgcgcccacc gccaggaccg cgcgcagggg cgccgcgtcc gacgaccggg
    12241 gcaccgagcc gggccaccgc gatcggacgg cccgggctgc gaccaggccc agtgcgacga
    12301 cgagcacgac cgcaatcaac cccgtgacgg ccgaccagcc gacgccgacg gcacgcaccc
    12361 cgagcccggc agcgatgatc acggcaacgc tgacggccgg agcagcgagc accgcggtga
    12421 gccaaccgat gccgaccgaa cgcagcagaa cggctccggg tacgaagaac accgcgacag
    12481 ccaccaggac cgccggcagc aattgcggcc aggacatcgt cacgagttct gaactcagct
    12541 catccgggcc gcgacatgca gccgccgcgc ggcctcgccg atcgagccgg tcatcgaggg
    12601 atagacggtg aaggcgttgg acagttggtc ggcggtcagg ccattctcga cggcgatcgt
    12661 cagcgggtag atcaactccg acgctcgcgg cgcgacgacc accccgccga gcaccgtgcc
    12721 gtgtccagac cgggcgaaga tcttgacgaa tccgtcgtgg aagccgtcca tcttggcccg
    12781 agcgttggtc gtcaacggca gtgtgatgac gatcgcgtcg actgtccccg cgtcgacgtc
    12841 ctgctgggac accccgaccg tcgcgatctc ggggtcggtg aagacgttgg ccgacacgac
    12901 gtccagccgc agcggggcga cggcgtcgcc gagcgcgtgc cacatggcga tccggccttg
    12961 catcgcggcc acggacgcga ggggcagcac cccggtgcag tcgcccgcgg catacacccc
    13021 gcgaactgac gtgcgcgaga ccccgtcgac gaggacgtgc ccgcccggac cgacctccac
    13081 accgacgtcc tcgaggccga gccccgcggt ctgcgggacg gacccgacgg ccagcagggc
    13141 gtggctgcct tcgacggtgc gaccgtcttc gaggcgcacg atgacaccgt cggccgtgcg
    13201 ctcgacggag ctcatccggg atcggccgag gacctccatg ccctcgcggc ggaagacctc
    13261 ctcgatgacg gcggcggcgt cgctgtcctc accagggagg acctggtggc gggaggacac
    13321 cagcgtcacg gcgctgccga gacccaggta
//
LOCUS       DAORPW010000057        13210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_969_length_13210_cov_675.589586, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000057 DAORPW010000000
VERSION     DAORPW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_969_length_13210_cov_675.589586"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1205)
                     /locus_tag="PLB86_06480"
     CDS             complement(87..1205)
                     /locus_tag="PLB86_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64817.1"
                     /translation="MTDHTQFRAVLRGYDPAQVDQVVAELTAAAEAARQEAATVSVQA
                     SKLEAGHADLRTQLEAREAQLTKLRESNRTAAAPSFAGLGDRVGQILTLADEEAASLR
                     TTAAAEIAALRTSSENAAAASRADADRYAEDVRTKADAESARILEDAKRKADEILDHA
                     DREASARREEAEAIYEHQRAKAAESAAEFETTLAARRDKAAAEFAAAMSQQEASLVAV
                     QDRASALASEAERSHHTAKSDAATLLEQARSEAGALVASAREQAERIKRDSDRELAAA
                     TARRDSITAQLTNVRQMLATLGGAGASMFSPLTGHDEPTPVTAATAEAVTVEAVTAEA
                     DAVEQSVAQDTDVEQIVDIPDLAEELLAEADGKAGRRG"
     assembly_gap    1341..1440
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1441..2513)
                     /locus_tag="PLB86_06485"
     CDS             complement(<1441..2513)
                     /locus_tag="PLB86_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64818.1"
                     /translation="MSDGSAYANPSVSAGGDVGDVTKHALEILARAQAHADTVKAQAD
                     EVFSAAQTRARELTAEAERLQREASALHAAAVSDRDQAAAVLASAHDDATSLLRDAAA
                     QASLVADAAARSSQAEIEEARRDAEALRAAALSDAEQQRRAAQVDFDTAMARFAAHEA
                     ERSVALDERLSAVEVEEANRLSMANTSASALVAAATADAERRTAAAREEADFIQAAAE
                     ALRERTVAETAQLRRQAAAEAQSRTASARADSDAMLSAAQAHLDWAERTMREIIDGAN
                     AEAERVTMTTARSLADHSRRRRQQLQGVLARVSHTSRQRLDDAAAESSRNRAQAEAIL
                     EAAAADAKRTEAEAAALAARLVA"
     gene            2662..4023
                     /gene="pafA"
                     /locus_tag="PLB86_06490"
     CDS             2662..4023
                     /gene="pafA"
                     /locus_tag="PLB86_06490"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HRC64819.1"
                     /translation="MKRRIFGVETEFGVTCTHEGQRRLTPDEVSRYLFRRVVAWGRSS
                     NVFLGNGSRLYLDVGSHPEYATAECDSIREVVVHDKAGERILEGLAEEAQQRLADEGI
                     AGDIYVFKNNTDSAGNSYGCHENYLIGRTGEFSLVSDMLIPFLISRQIICGAGKLASG
                     SKRATYCVSQRADHIWEGVSSATTRSRPIINTRDEPHADAEKFRRLHVIVGDSNMSET
                     TTLLKVGSADLVLRMAETGVVMRDLALENPIRAIREMSHDMTGRRTVRLADGRSMSAL
                     DMQSEYFTRVRAFADREGLTDGAHARVLELWERTLHAVETNNLALVEREIDWVIKWRM
                     IERYAAKHQLSLDDPRLAQLDLAYHDVNRRRGLFYLLQRRGLVTREASDLEVFRAKTV
                     APQTTRARLRGEFVKAAQAKRRDFSVDWVHLKLNDQAQRTVLCKDPFASVDARVDRLI
                     EAM"
     assembly_gap    4216..4315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4316..4951
                     /locus_tag="PLB86_06495"
     CDS             <4316..4951
                     /locus_tag="PLB86_06495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRC64820.1"
                     /translation="PQSFALNPKNYLPGLANGLAGLNLGSRILLAIPASEGFGTAGRP
                     DLGISGTETLVMVVDIVDVANAIPQATGTPVAPVTGQPTVTFDPTTGPTITIPAGAAA
                     PTTLVNQNLIEGSGTKVTEGMFLQVHYTGVLWKDGSVFDSSWPRAQYYTFQTGTNSVI
                     PAWDKGLVGHTVGSRVLLVVPPADGYGPDGNPPKIAGTDTLVFVVDILAAA"
     gene            5026..5412
                     /locus_tag="PLB86_06500"
     CDS             5026..5412
                     /locus_tag="PLB86_06500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRC64821.1"
                     /translation="MSLDPNRSKPELDYPGDAVPTDLVIEDLIEGDGAAVEAGQTVTT
                     HYVGWAFSTGEEFDASWNRGEPLSFRAGIGQVIRGWDQGLLGMKVGGRRKLTIPSHLA
                     YGERGAGGVIKPGESLIFVVDLVAVR"
     gene            5428..6447
                     /locus_tag="PLB86_06505"
     CDS             5428..6447
                     /locus_tag="PLB86_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRC64822.1"
                     /translation="MSVQPSTAAKTERLLNLVIALLSTRRPLTKAQLRTAVPHYQQTA
                     SDEAFDRMFERDKDELRDLGIPLATQLVAAAYDDEVGYRIDRREYALPEIAFEPDEVA
                     ALALAGRAWNQASLASSAAAALTKLASAGVELDERSVVGIEPRVRTAEAAFASVHAAV
                     KARREISFVYRKANGTVDTRRLQPWGMTQWRGRWYVTGMDVDRQAERVFRLGRIEGKV
                     SMTARVAAYEIPTDHVPRVLVAASEPPVVDQPPAVLRIRRGAGNTLRRRARTVGEVDD
                     EWSQIDLDYRDPEGLAQEVAGFGPDVVVEAPDSVRDAVIRLLGAARERHTATPADANA
                     PGGMP"
     gene            6444..7505
                     /locus_tag="PLB86_06510"
     CDS             6444..7505
                     /locus_tag="PLB86_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRC64823.1"
                     /translation="MTATRAGRPVQESATARLSRLLTMVPWLLARQGVDVDVAAAQFG
                     VSREQLESDLALLFVCGTPGHLPDDLIEADWEDGQIYVGNADAISRPLRFTVDEALTL
                     MVGLRALAATPGVTGASGADGGEAILRALTKLEQATGEAAGVAARVRVTLDDGAAETI
                     LADASRAVQERRRVRLSYQSFGRDEVTEREVDPMRVTSLDARWYLEGWCHRAEDVRLF
                     RLDRVQSLQVLDVDGTPPESAQLRDLAGETYQPSPDDVLVELLVEPAAAWVADYYPVD
                     SVADAGDGRRRILLRAADPSWVRRLAWRLGGHVQILAPQSLAEQVAAGAQAALAAYPV
                     QVVQGLAPAGMPPASDDVR"
     gene            7505..7861
                     /locus_tag="PLB86_06515"
     CDS             7505..7861
                     /locus_tag="PLB86_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64824.1"
                     /translation="MRGLVWTLIWLALVAGAAWYLWRRLRWLWGRTEMLGDTLAEAET
                     LFAAATEREVDPRDGVTDHRPQVELAIFRAPADVAQERDLIRETLRGERAARRRASLP
                     GWARRVDSIDADHRKA"
     gene            7867..8106
                     /gene="tatA"
                     /locus_tag="PLB86_06520"
     CDS             7867..8106
                     /gene="tatA"
                     /locus_tag="PLB86_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HRC64825.1"
                     /translation="MFLREPSHWLIVVLALVLLFGWKRLPDMARSVGRSMRIFKSEVN
                     EMKNDGKEGRPSPAAEDVVPGDVVPPRSTDDRPSV"
     gene            8158..9000
                     /gene="tatC"
                     /locus_tag="PLB86_06525"
     CDS             8158..9000
                     /gene="tatC"
                     /locus_tag="PLB86_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRC64826.1"
                     /translation="MPSRTHMARFRFRRNRDPEGRMSLGDHLRELRRRTVISALAIVV
                     GAVFGWTRWVAIYDALTSPVTEVARTKTAGALTTVSFSTPTDSFSVMITVGLFVGVVV
                     ASPVWLWQIWAFIVPGLTRKEKRITVAFIGAAVPLFLGGCWLAYAVLPRAMGALLSFT
                     PTSGSNIIDVSLYLSFVLKFILVFGLGFLLPVFLVGLNVAHILPGRLMLRAWRGAVFM
                     CFLFTAVMTPDPNPWTMVILAVPLVMLYFAAVGVSLLLDRRRARSDPSAEWLRVADDE
                     ASAL"
     gene            9010..9918
                     /locus_tag="PLB86_06530"
     CDS             9010..9918
                     /locus_tag="PLB86_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HRC64827.1"
                     /translation="MTHFTGPLRRIGLYANPVSGRGRSETAAAQLRGILADSGHIVLD
                     IAAQSAAEGRVRTFDAVRSGAIDALVVAGGDGTANLGANACAGTDIPLVVLPAGTGND
                     NARSLGMPRKDIPAIARLISDGQTRRIDAGRCVTADGELWWLGVLGGGFDTLVNTRGR
                     SLTALHGTPRYLAAVALELPRFTGIPYVVQVDDQRIETEAMLVAVANGGTFGGGMKVC
                     PDASMTDGLFDVMILHKIGRGEFLRIFPKVFSGGHVSHPAVEILRGRRVHLEADGIDS
                     QADGEDFLPLPVDLEVVPGALAVVAP"
     gene            9915..12725
                     /locus_tag="PLB86_06535"
     CDS             9915..12725
                     /locus_tag="PLB86_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC64828.1"
                     /translation="MSELTRFAAGLDFGLDPFQVAGCEALEAGRGVLVAAPTGAGKTV
                     VGEFAVHLALATGRKAFYTTPIKALSNQKYADLCRRHGAANVGLLTGDSSVNGEAPVV
                     VMTTEVLRNMMYAGSPTLAGLGYVVMDEVHYLADRFRGAVWEEVIIHLPEDVLVVSLS
                     ATVSNAEEFGEWLGEVRGDVAVVVSEIRPVPLWQHVMVGKQLFDLFVEESLDSSGRPQ
                     EGRRVQVNPELLERIRSVERALAADSAAHRGLRGRTGPGGGPPGGGRGPRGGRGSWES
                     DSSARGREGAIRPGGGPTRADVIATLDREGLLPAITFVFSRNGCDAAVGQLLARGIRL
                     VSEADGVRNRRYVEERIGALADEDLSVLGYWDFVDGIERGFAAHHAGMLPTFREIVEE
                     LFTQGRVRAVFATETLALGINMPARTVVLEKLVKYNGDTHAELTPAEYTQLTGRAGRR
                     GIDVEGHGVVLWSRGLDPLVVGGLASTRTYPLRSSFRPTYNMAVNLVAQVGRDRAREL
                     LETSFAQFQADRSVVGLAQSVRRGERQLAEYAVEWHCHLGDFGEYAALRASLRDLEKG
                     AAKARSAATRAEAELSLERLRPGDVIRVPTGRRAGYAVVVAANRGAPGRPASPAVVTE
                     DRQLRRLTTVDVPTPVQPLLTVRIPTGFNAKSPKSRRDLAATLRIAVAHEPPPRHTSR
                     GGDDDARRESDAEVTRLRAALREHPCHACPDREEHSRRAEKWWRLRRETDALARQVDG
                     RTNTVARTFDRICDLLEDLGYLGEGGLTVTEAGQDLRQIYAERDLLAAQCIREGVWNR
                     LDAAGLAAVVSALIHEPRREDLAAAPRVPTTDVGEALTAMTALWATLEEQERLHGLPP
                     SGAPDAGIVWMVHRWASGQRLETVLRGSDLAAGDFVRRCKQVVDLLGQISDIAPHPAL
                     SAKARKAVDAVRRGVVAADRID"
     gene            12918..>13210
                     /locus_tag="PLB86_06540"
     CDS             12918..>13210
                     /locus_tag="PLB86_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRC64829.1"
                     /translation="MQQLTGLDEMFLSLDTGRTTGHVAGIAFFDKPAEKRDELAFLRE
                     RVADRLTRLPVLRWRLKSVPLEMDGRYWVDGPVDIKEQVIGIRLPKPGTDAQL"
BASE COUNT         1865 a         4563 c         4521 g         2061 t
ORIGIN      
        1 ccccgtggtg tcagcacacg gcatacgacg aaggcggccc ggaccacact ggtctgggcc
       61 gcctccggtg cttttgcgcg tggggctcag ccgcgccgac cggccttgcc gtcggcctcg
      121 gcgagcagtt cctcggccag gtcggggatg tcgacgatct gctcgacatc ggtgtcctgg
      181 gccacggact gctcgaccgc gtcggcctcg gcggtgactg cttcgacagt gactgcttcg
      241 gcggtggccg cggtaaccgg cgtcggctcg tcgtgaccgg tgagggggct gaacatcgac
      301 gcgccggcgc cgccgagggt ggcgagcatc tggcggacgt tcgtcaactg cgcggtgatc
      361 gagtcgcgtc gggccgtggc cgcagccaac tcgcggtcgg agtcgcgctt gatccgttcg
      421 gcttgctcac gggccgatgc gacgagcgcg ccagcctccg agcgggcctg ctccagcagc
      481 gtggcagcgt cggacttggc ggtgtggtgc gaccgctccg cctccgacgc cagcgccgat
      541 gcgcggtcct gaacggcgac cagggaggcc tcctgctggg acatggcagc ggcgaactca
      601 gcagcggcct tgtcgcggcg agcggccaac gtcgtctcga actcggcggc cgactcggcg
      661 gccttcgcgc gctggtgctc gtagatcgct tcggcctcct cgcgtcgagc cgacgcctcc
      721 cgatcggcat ggtcgaggat ctcgtcggcc ttgcgcttgg cgtcttcgag gatccgggcc
      781 gactcggcgt cggccttggt ccggacgtcc tcggcgtagc ggtcggcgtc ggcgcgggag
      841 gcggccgcgg cgttctccga cgaggtccgc agggcggcga tctcggcagc ggcggtcgtg
      901 cgcagcgagg cggcctcctc gtcggcgagg gtgaggatct ggccgacccg gtcaccgagc
      961 cccgcgaagc tgggggctgc ggcggtgcgg ttgctctcgc gcagcttcgt caactgagcc
     1021 tcacgggcct ccaactgggt gcgcaggtcg gcgtgccctg cttccagttt gctggcctgg
     1081 acgctgacgg tggcggcttc ctgcctggcg gcttcggcgg cggcggtgag ttcggccacc
     1141 acctggtcga cctgagcggg gtcgtagcca cggagcacgg cgcggaactg agtgtggtcg
     1201 gtcatcaaga tcccatcggg tgaatgcgaa ggtcggtcag gagagagggt gaatcagtga
     1261 tcggtcatcc gcggagtagg cgatgacctc gacgggctcc gcgtcgtctt gctcttcggc
     1321 acggtggacg gcgcggtagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 tcggcgacga gccgggcggc cagcgcggca gcctcggcct cggtgcgctt ggcgtcggct
     1501 gctgccgctt cgaggatcgc ctcggcctgg gcgcggttgc ggctggactc cgcagctgca
     1561 tcgtccagtc gctgccgcga ggtgtgggag acgcgggcca gaacaccttg gagttgctgg
     1621 cgccgccgcc gcgagtggtc ggccagggac cgggccgtcg tcatcgtcac tcgctcggcc
     1681 tcggcgttgg cgccgtcgat gatctcgcgc atggtccgct cggcccagtc aaggtgggct
     1741 tgggcggcgc tgagcatcgc gtcggagtcc gcgcgggccg acgcagtgcg agactgcgcc
     1801 tccgccgcgg cctggcggcg caactgggcc gtctcggcga cggtgcgctc gcgcagtgcc
     1861 tcggcggcgg cctggatgaa gtcggcctct tcacgcgctg cagcggtgcg tcgctcggcg
     1921 tctgcggtgg cggcagcgac gagcgccgac gcgctcgtgt tggccatcga caggcggttg
     1981 gcttcctcaa cctcgacggc agacaaccgc tcgtccagcg ccaccgaacg ctcggcctca
     2041 tgagcggcga atcgggccat cgcggtgtcg aaatcgacct gagcggcacg ccgctgctgc
     2101 tcggcgtcac tcaaggctgc ggcccgcaac gcttcggcgt cgcgacgagc ctcctcgatc
     2161 tcggcctggc tgctgcgcgc ggccgcgtcg gcaacgagag atgcctgggc tgccgcgtcc
     2221 cgcagcaacg aggtggcatc gtcgtgggcc gacgccagca ccgcggcggc ctggtcgcgg
     2281 tcggagaccg cggcagcgtg cagtgcgctg gcctcccgtt ggagtcgctc ggcctcggcg
     2341 gtgagctcgc gggcccgagt ctgggcagcc gaaaagacct cgtcggcctg cgccttgacg
     2401 gtatcggcgt gggcttgggc acgcgcgagg atctccaacg cgtgcttggt cacatctccg
     2461 acatcgcccc cggcggagac cgacgggttg gcgtacgccg aaccgtcgga catgtggaca
     2521 tgatctcaga tatgggcgct acttcccaca cccgcgtgtg tcggccaatt ctcggcccaa
     2581 cggacgaact gtctggtcct gtgggcctac tggcgcccaa ggtccctcgg gacgcggcat
     2641 acctgcccgt agggtgaggg aatgaagcgg cggatcttcg gagtcgagac cgagttcggg
     2701 gtgacctgca cccacgaagg acagcgacgt ctgacccccg acgaggtgtc gcgttacctc
     2761 ttccgccgcg tcgtcgcgtg gggacgcagc agcaacgtgt tccttggcaa cgggtcgcgg
     2821 ctctatctcg acgtcgggag ccacccggag tacgccaccg ccgagtgcga ctcgatccgc
     2881 gaggtggtcg tccacgacaa ggcgggcgaa cggatcctcg agggcctggc cgaggaggcc
     2941 cagcagcgcc tggccgacga ggggatcgcc ggcgatatct acgtcttcaa gaacaacacc
     3001 gactcggcgg gcaactccta cggctgccac gagaactacc tcatcgggcg caccggagag
     3061 ttctcgctcg tctcagacat gctcatcccc ttcctgatca gccgtcagat catctgtggc
     3121 gcaggcaaat tggccagcgg cagcaagcga gcgacctact gcgtcagcca gcgggccgac
     3181 cacatctggg agggtgtctc gtcggcgacg acgcgcagtc gcccgatcat caacacccgc
     3241 gacgagccgc acgccgacgc cgagaagttc cggcgcctgc acgtcatcgt cggcgactcg
     3301 aacatgtccg agaccacgac gctgctcaag gtggggtccg ccgacctggt cctgcgcatg
     3361 gccgagacgg gtgtcgtcat gcgcgacctc gcgctggaga accccatccg ggcgatccgc
     3421 gagatgagtc acgacatgac cggtcgccgg accgtccggt tggccgacgg ccgctcgatg
     3481 tcggcgctgg acatgcagtc cgagtacttc acccgggtgc gggctttcgc cgatcgcgag
     3541 gggctgaccg acggcgccca cgcgcgggtg ttggagttgt gggagcgcac cttgcacgcc
     3601 gtcgagacca acaacctcgc cctggtggag agggagatcg actgggtcat caagtggcgc
     3661 atgatcgagc ggtatgccgc caagcaccag ttgtcgctgg acgacccacg cctggcccaa
     3721 ctcgacctgg cttaccacga cgtcaaccgt cgacgtgggc tgttctacct gctccagcgt
     3781 cgcggcctgg tcacgcgtga ggccagcgac ctcgaggtct tccgcgccaa aaccgtggcg
     3841 ccgcaaacca cccgggcgcg gctgcggggc gagtttgtca aggccgccca ggccaaacgc
     3901 cgcgatttca gcgtcgactg ggtccacctc aagctcaacg atcaggcgca acgcaccgtc
     3961 ctgtgcaagg acccgttcgc cagcgtcgac gcccgggtcg accgactcat cgaggccatg
     4021 tagggctccc tgcggccagg cgcccgtccg ccgccacagg acacccggtt agagtggcgg
     4081 ggtttgcccg tccccttcac gacccgaggt cctccccgtg ccccagccgt atgccgccca
     4141 ccgcctccgc gcccagacgc tgcggccgcg tcctcacctg agcgtcgtcg gcctcgccgt
     4201 caccgccctg gtggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncctca
     4321 gtcgttcgcg ctgaacccca agaactacct tcctgggctg gccaacggcc tggccgggct
     4381 gaacctcggc tcgcggatcc tgttggccat cccagcttcc gagggcttcg ggacggctgg
     4441 ccgcccggac ttgggcatct ccggaaccga gaccctcgtc atggtggtgg acatcgtcga
     4501 cgttgccaac gccatccctc aagcgaccgg cacgcccgtc gcgccggtca cgggccagcc
     4561 aacggtgacc ttcgacccaa ccacggggcc aaccatcacc atcccggccg gtgcggctgc
     4621 accgacgacc ctggtcaacc agaacctcat cgaggggagc ggcacgaagg tcaccgaggg
     4681 tatgttcctc caggtccact acacgggagt gctgtggaag gacggcagcg ttttcgactc
     4741 ctcgtggccc cgagctcagt actacacctt ccagaccggc acgaactcgg tgatccccgc
     4801 gtgggacaag ggcctggtgg gtcacaccgt cggctcgcgc gtcctgctgg tcgtcccgcc
     4861 ggccgatggg tatggcccgg acggaaaccc gcccaagatc gccggcaccg acacgctcgt
     4921 cttcgtcgtc gacatcctcg ccgcagcctg accgcgtcac tcactgaccg cttcactcac
     4981 tgaccgcctc accccctgac cactccgccg acaggagaaa ccgacatgtc tctcgacccc
     5041 aaccgcagca agcccgagct ggactacccc ggggacgccg ttcccaccga cctggtcatt
     5101 gaagacctca tcgagggtga cggcgcagcc gtcgaggccg gccagacggt caccacccac
     5161 tacgtcggat gggcgttctc gaccggcgag gagttcgacg cctcgtggaa ccgcggtgag
     5221 ccgctgtcgt tccgcgccgg catcggtcag gtcatccgtg gatgggacca ggggctgctg
     5281 gggatgaagg tcggcggtcg tcgcaagctg accatcccct cgcacctggc ctacggcgag
     5341 cgtggcgcag gtggcgtcat caagccgggg gagtcgctca tcttcgtggt cgacctcgtc
     5401 gccgtccgct gaggtagcgt cgggagggtg agcgtccagc cgagcaccgc agccaagacc
     5461 gagcgactgc tcaacctggt catcgccctg ctctctaccc gtcggccact caccaaggcc
     5521 cagttgcgca cggcggtccc gcactaccag cagaccgcgt cggatgaggc ctttgaccgg
     5581 atgttcgagc gggacaagga cgagttgcgc gacctgggga tccccttggc cacccaactc
     5641 gtcgcggcgg catacgacga cgaggtcggt taccgcatcg accggcgcga gtacgccttg
     5701 cccgagatcg cgttcgagcc cgacgaagtc gccgcgcttg ccctggccgg ccgggcctgg
     5761 aaccaggcga gcctggcctc ttccgcggcg gccgcgctca ccaaactggc ctctgccggg
     5821 gttgagctgg atgagcgttc cgtcgtcggg atcgagcccc gggtccgcac cgccgaggcg
     5881 gccttcgcgt ccgtgcacgc ggcggtcaag gcccgacgcg agatctcctt cgtctatcgc
     5941 aaggccaacg gcacggtcga cacccggcga ctgcaaccct gggggatgac ccagtggcgt
     6001 ggccgctggt acgtcaccgg gatggacgtc gatcgccagg ccgaacgggt gttccgcctc
     6061 ggccggatcg agggcaaggt gagcatgacg gcgcgggtcg cggcatacga gatcccgacc
     6121 gatcacgtcc cgcgggtcct cgtcgccgcg tccgagccgc ccgtcgtcga ccagcctccc
     6181 gcggtcctgc gcatccgccg aggcgccggc aacacgctgc gccgccgggc ccgcacggtc
     6241 ggcgaggtgg atgacgagtg gtcccagatc gacctcgact accgcgaccc cgagggcctg
     6301 gcccaggaag ttgccgggtt cggcccggat gtcgtcgtcg aagcgcccga cagcgtgcgc
     6361 gacgcggtga tccgcctgct cggtgcggcg cgcgagcggc ataccgcgac gcccgctgac
     6421 gccaacgcgc ctggaggtat gccgtgaccg ccacccgcgc aggccgcccc gtgcaggaga
     6481 gcgccaccgc gcggctgtcc cggctgttga ccatggtgcc gtggctgctg gctcgccaag
     6541 gcgtcgatgt cgacgtggcg gccgctcaat tcggagtgag ccgcgagcaa ctcgagtccg
     6601 acctggcgct gctgttcgtc tgcggcaccc ccggccacct gcccgacgac ctcatcgagg
     6661 ccgactggga ggacggacag atctatgtcg gcaatgccga cgccatctca cgcccgctgc
     6721 ggttcaccgt cgacgaggcc ctcaccctca tggtggggct gcgtgccctc gccgcgaccc
     6781 ccggagtcac gggagcaagc ggcgcggacg gcggagaagc gatcctgcgg gccctgacca
     6841 agctcgaaca ggcgacaggt gaggcggccg gtgtcgccgc gcgggtgcgg gtcaccctcg
     6901 acgacggcgc tgccgagacg atcctcgccg acgcctcccg agccgttcag gagcgccgcc
     6961 gcgtgcggct gagctatcag tcgttcggtc gtgacgaggt gaccgagcgc gaggtcgacc
     7021 ccatgcgggt gaccagcctg gatgcccgtt ggtatctcga ggggtggtgc caccgggccg
     7081 aggacgtgcg gctgttccgc ctggatcggg tgcaatcgct gcaggtcctc gacgtcgacg
     7141 ggaccccgcc ggagtcggcc caactgcgcg acctcgccgg cgagacctac caaccctcgc
     7201 cggacgacgt gctcgtcgag ttgctcgtcg agccggcggc cgcgtgggtc gctgactact
     7261 accccgtgga ctccgtggcc gatgccggcg acggacgccg ccggatcctc ctgcgcgccg
     7321 ccgaccccag ttgggtccgc cgattggcct ggcgcctggg cgggcacgtg cagatcctcg
     7381 ccccgcagtc gctggccgag caggtggccg ccggcgcgca ggcagcgctc gcggcatacc
     7441 ccgtgcaggt cgtgcaaggg ctggcaccgg cgggtatgcc gcccgcctcc gatgacgtgc
     7501 gctgatgcgc ggcctcgtct ggacgctgat ctggctcgcg ctggtcgctg gggccgcctg
     7561 gtatctctgg cgccggctgc ggtggctgtg gggccggacc gagatgttgg gcgacaccct
     7621 cgccgaggct gagaccctct tcgcggcggc gacggaacgc gaggtcgacc cccgcgatgg
     7681 ggtgaccgat caccgacccc aggtcgagtt ggcgatcttt cgcgcacccg ccgacgtggc
     7741 tcaggaacgt gacctcatcc gagaaacgtt gcggggggag cgagcggcgc gtcgtcgggc
     7801 atccctacca gggtgggcac gccgcgtaga ctcgatagac gccgaccatc gaaaggcctg
     7861 accagcatgt ttctccgtga acccagccac tggctcattg tggttcttgc tctcgtcctg
     7921 ctcttcggct ggaagcgcct gcccgacatg gcccgcagcg tggggcgctc gatgcggatc
     7981 ttcaagtccg aggtcaacga gatgaagaac gacggcaaag agggtcggcc cagcccggcc
     8041 gccgaggatg tcgtccccgg ggacgtcgtc ccgccccggt cgaccgacga ccgcccctcg
     8101 gtctgagagg acactcggcg cacctgagtc gccgcccccg ggcggtcgct ctcgtgagtg
     8161 ccgagccgca cccacatggc ccgcttccgg ttccgccgca accgtgaccc cgaaggacga
     8221 atgtccctcg gggatcactt gcgtgagttg cgccgacgga cggtcatctc ggcactggcg
     8281 atcgtcgtcg gcgcggtgtt cggctggacc cgttgggtgg cgatctacga cgctctgacc
     8341 agcccggtga ccgaagttgc tcggaccaag accgcaggcg cgctgacgac agtctcgttc
     8401 agcacgccca ccgactcgtt ctcggtgatg atcaccgtcg ggctgttcgt cggggtggtg
     8461 gtcgcgtcgc cggtctggct gtggcagatc tgggcgttca tcgtccccgg cctcacgcgc
     8521 aaggagaagc gcatcaccgt ggccttcatc ggggccgcgg tgccgttgtt cctcggcggc
     8581 tgctggttgg cgtatgccgt gttaccgagg gccatgggag ccctcctcag cttcacgccg
     8641 acgtcggggt cgaacatcat cgacgtctcg ctctacctca gcttcgtcct caagttcatc
     8701 ctcgtcttcg ggttgggctt cctgctgccg gtcttcctcg tcggtctcaa cgtcgcgcac
     8761 atcctccccg gtcgactgat gctgcgagcg tggcgcgggg cggtcttcat gtgcttcctg
     8821 ttcaccgccg tcatgacccc ggaccccaac ccgtggacca tggtcatcct cgccgtcccg
     8881 ctcgtcatgc tctatttcgc ggccgtcggg gtgtcgctgc tgctggaccg tcgacgggcc
     8941 cgctccgacc cctccgcaga gtggctccgc gtcgctgacg acgaggcctc cgccctgtaa
     9001 cctcgccgcg tgactcactt caccgggccg ttgcgcagga tcgggctcta cgccaacccc
     9061 gtgtctggtc gcggacgaag cgagacggcc gcggcgcagc tgcgcggcat actcgccgat
     9121 agcggtcaca tcgtcctgga catcgccgcg cagtcggcgg cggaggggcg ggtgcgcacc
     9181 ttcgacgccg tccggtccgg ggccatcgac gccttggtcg tcgccggcgg cgacggcacg
     9241 gccaacctcg gcgccaacgc ctgcgcgggc acggacatcc ccctcgtcgt cctcccggca
     9301 ggcaccggca acgacaacgc ccgcagcctc ggtatgccgc gcaaggacat ccccgcgatc
     9361 gcgcggctca tctccgacgg ccagacgcga cgcatcgacg ccggccgctg tgtgaccgca
     9421 gacggcgagc tctggtggct cggggtcctc ggtggcgggt tcgacaccct cgtcaacacc
     9481 cgcggtcgca gcctcaccgc cctgcacggc acaccgcgtt acctcgccgc cgtcgccctg
     9541 gaactgccgc ggttcaccgg catcccctat gtcgtgcagg tggacgacca gaggatcgag
     9601 acggaagcga tgctcgtcgc cgtggccaat gggggaacct tcggcggtgg gatgaaggtc
     9661 tgccccgacg cctcgatgac cgacgggctg ttcgacgtga tgatcctgca caagatcggg
     9721 aggggtgaat tcctcaggat cttccccaag gtcttctccg gcggccacgt gagccacccg
     9781 gcggtcgaga tcctgcgcgg gcggcgggtg cacctcgagg cggacggcat cgacagccag
     9841 gccgacggag aggacttcct gccgctgccg gttgaccttg aggtcgttcc cggggccctc
     9901 gccgtcgtcg caccgtgagt gagctgaccc ggttcgctgc cgggctcgac ttcggtctcg
     9961 atcccttcca ggttgcgggc tgcgaggccc tggaggccgg tcgcggggtc ctggtcgcgg
    10021 caccgaccgg tgccggcaag accgtcgtcg gcgagttcgc cgtgcacctg gccctggcca
    10081 ccggccgcaa ggcgttctac acgacgccga tcaaggccct gtccaaccag aagtacgccg
    10141 atctctgtcg gcgccacggc gccgccaacg tcgggctgct caccggggac tcctccgtca
    10201 acggcgaggc tcccgtcgtc gtcatgacga ccgaggtgct gcgcaacatg atgtatgccg
    10261 gttcgcccac cttggcgggc ctcggttacg tcgtcatgga cgaggtccac tacctcgccg
    10321 accgcttccg gggtgctgtc tgggaagagg tgatcatcca cctgcccgag gacgtcctcg
    10381 tcgtctcgct gtcggcgacg gtgtccaacg ccgaagagtt cggcgagtgg ctgggggagg
    10441 ttcgcggcga cgtggccgtc gtcgtctctg agatccgccc ggtgccgctg tggcagcacg
    10501 tcatggtcgg taaacaactc ttcgacctgt tcgtcgagga gtccttggac tcctccggcc
    10561 gaccgcagga gggacgccgg gtccaggtca accccgagtt gttggagcgg atccgctcgg
    10621 tcgagcgagc gctggccgcc gactccgccg cccatcgcgg actgcgcggg cggaccgggc
    10681 ctggcggagg gccgcccggc ggcggacgcg ggccacgagg cgggcggggg tcctgggagt
    10741 cggactcatc agcgcgaggg cgggagggcg ccatacggcc cggtggtgga cccacccgtg
    10801 ctgacgtcat cgcgaccctc gaccgcgaag ggctgttgcc ggcgatcacg ttcgtgttca
    10861 gccgcaacgg gtgcgatgcc gctgtcgggc aactgctggc gcgggggatc aggctcgtca
    10921 gcgaggcgga cggtgtccgc aaccggcggt atgtcgagga gcggatcggc gccctggccg
    10981 acgaggacct gagcgtcctg ggctactggg acttcgtcga cggcatcgaa cggggcttcg
    11041 cggcccacca cgccgggatg ttgccgacct tccgcgagat cgtcgaggag ttgttcaccc
    11101 agggccgggt ccgggcggtc ttcgccaccg agaccctggc cctcgggatc aacatgcccg
    11161 cgcgcacggt cgtcctggag aaactcgtca aatacaacgg cgacacccac gccgagctca
    11221 cccccgcgga atacacccaa ctcaccggtc gtgccggccg gcgcggcatc gacgtcgaag
    11281 gacacggcgt cgtcctgtgg agccgtggcc tggatccgct cgtcgtcggc gggctcgctt
    11341 cgacgcgcac ctacccgttg cggtccagct tccgccccac ctacaacatg gccgtcaacc
    11401 tcgtcgccca ggtcggtcgc gatcgtgcgc gtgagctgtt ggagacgtcg ttcgcgcagt
    11461 tccaggccga ccgttccgtg gtcggcctgg cgcagtcggt gcgccgcggc gagcggcagt
    11521 tggccgagta tgccgtcgaa tggcactgtc atctgggcga tttcggggag tatgccgcgc
    11581 tgcgcgcctc cctgcgtgac ctcgagaagg gcgcggcgaa ggctcggtcc gccgcgacgc
    11641 gggccgaggc cgaactgtcc ctggaacggt tgcgtcccgg cgacgtgatc cgggtgccga
    11701 ccggccgacg cgccgggtat gccgtggtcg tcgccgccaa ccgtggggct ccgggacgcc
    11761 cggcctcacc ggcggtcgtc accgaagacc gccaactgcg tcggctgacc accgtcgacg
    11821 tcccgacgcc ggtgcaaccg ttgctcacgg tgcgcatccc gaccgggttc aacgccaaga
    11881 gccccaaatc gcgacgcgac ctggccgcca cgctgcggat cgccgtggca cacgagccgc
    11941 cgccgcggca tacctcccgg ggtggtgacg acgacgcgcg ccgggagtcc gacgcggagg
    12001 tcacccgact gcgcgcggcc ctgcgggagc acccctgcca cgcctgcccc gaccgggagg
    12061 agcactcccg gcgggccgag aagtggtggc ggctgcgccg ggagaccgat gcgctcgcgc
    12121 gtcaggtcga cggccgcacc aacaccgtcg cgcgcacctt cgaccggatc tgtgacctct
    12181 tggaggatct ggggtatctc ggcgagggcg ggctcacggt caccgaggct gggcaggacc
    12241 tgcgccagat ctatgccgaa cgcgacctcc tggccgctca gtgcatccgc gagggagtgt
    12301 ggaaccggct tgatgccgcc ggcctggctg ccgtcgtgtc ggccctcatc cacgaaccgc
    12361 gtcgcgagga cctcgctgcc gccccgcgcg tgccgacgac ggacgtgggg gaggccctca
    12421 ccgcgatgac ggcgctgtgg gccacgttgg aggagcagga gcggctccac gggctgccgc
    12481 cgagcggcgc acccgatgcc gggatcgtct ggatggtgca ccggtgggcc agcgggcaac
    12541 gcctggagac ggtgctgcgg ggctctgacc tggccgccgg tgacttcgtg cgccgctgca
    12601 agcaggtggt cgacctgctg gggcagatca gcgacatcgc gccgcacccc gccttgagcg
    12661 ccaaggcccg caaggccgtt gacgcggtcc gccgcggggt ggtggccgcc gaccggatcg
    12721 actgaccgcg ttcagccggc cggatcgagt catcggagcg gccaaagcgc tgctgaggtg
    12781 cgcttctcat ggggtattcc acggtccgta gtgatgtccg ccatggcgga cggactgcca
    12841 cgatggggga tcgcctgaaa tactgtcaag gacttgatga ctgaattatc caacgatccc
    12901 cccttacgag gagagacatg cagcaactca ctggcttgga cgagatgttc ctttcgttgg
    12961 acaccgggcg aaccaccggg cacgtcgccg gtatcgcctt cttcgacaag ccggccgaga
    13021 agcgcgacga actggcgttc ctgcgcgagc gggttgccga ccggctgacg cgccttccgg
    13081 ttctgcggtg gcgcctcaag agcgtgccgc tggagatgga cgggcgctac tgggtggacg
    13141 gaccggtcga catcaaggag caggtcatcg gcatccggct gcccaagccc ggcaccgacg
    13201 cccaactcca
//
LOCUS       DAORPW010000058        13171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_971_length_13171_cov_777.172614, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000058 DAORPW010000000
VERSION     DAORPW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13171
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_971_length_13171_cov_777.172614"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1195
                     /locus_tag="PLB86_06545"
     CDS             149..1195
                     /locus_tag="PLB86_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HRC64830.1"
                     /translation="MRATTLHGAFDIRVSDLPDPKLHRPTDALVRVTASCVCGSDLWP
                     YRGINASKPGQRIGHEFVGIVEEVGSEVATVRPGDFVIAPFAWSDNTCRVCRVGIQTS
                     CENGGWWGGRDREGLPVDAGQGEFVRVPLADGTLVATPEVPSADLVPDLLTLSDVMGT
                     GWHCALSAGVRPGDTVAVVGDGAVGLCALLSARLQGATRIIAMSRHEARSAIAVEFGA
                     TEVVAERGEAGVARVRDLTDGFGVDAALECVGTAESMRTALDLVRPGGRIGFVGVPHD
                     VTVPIEDLFARNVHIGGGVAPVREYLPALRDLVLAGEIHPGRVFDLTVPLADVAEAYA
                     AMDQRRATKAMLLP"
     gene            1192..1623
                     /locus_tag="PLB86_06550"
     CDS             1192..1623
                     /locus_tag="PLB86_06550"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HRC64831.1"
                     /translation="MSNHGGEIAGTLIGLFWVLLGWWMIAVGRRRGRVGGDWVPAVGT
                     IVDKSGGTEGLFLRNPHIRYAAPDGSEHIVASRSHGDLWEPGQSVDILVDPTEPGRAA
                     LLTHAARATPYVVIGWFIIVVGLLTLIASLMLAVWVPPPST"
     assembly_gap    1648..1747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1878..2834
                     /locus_tag="PLB86_06555"
     CDS             1878..2834
                     /locus_tag="PLB86_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRC64832.1"
                     /translation="MLDGQPQSHVDLADPGLITFEYVQHFALAIGACSPTAPAPLRVT
                     HVGGAGLTLARWVQHTRPVSPQIVLEPDAALTAAVRERLPLPRGHRIRVRPLDGRTGV
                     RGLATASADVLVLDAYAAGRVPAELATPEFLADVARVLAPGGLLLANLADEPGLRWAA
                     RFLATLASVEGFGGPAVLAVLATHDVLKGRRFGNLVAVASRGADDLANPAPTDAGPAR
                     SDSARLSPTDAEANRSGSARLSPTDAEANRPDSSGLSRTGDGQPRLDLAALRRAGASA
                     PIPTGIRSGPEVTRLIAGARPFADADAQPSPVPPDPGAWRVR"
     assembly_gap    2915..3014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3260..3943)
                     /locus_tag="PLB86_06560"
     CDS             complement(3260..3943)
                     /locus_tag="PLB86_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRC64833.1"
                     /translation="MSVSGVGVSGRGRLVVFDCDGVLVDSERLNVALDVEAIGELGWA
                     ITREEVIARHVGLSEADATADIESVIGRPVPAEWVLRWRSAYHAAYETQLEAVPGVGA
                     AIADLVADGWAVCVATSGERATTWRKLARCGLAQWFTEATVFTAADVARGKPAPDLFL
                     WAGAQLGVAPERCVVVEDSPHGVAAARAAGMPCVGFAGSVTSRGQLVDADVVIDDMSA
                     LVAAICDLV"
     gene            complement(3950..7870)
                     /gene="purL"
                     /locus_tag="PLB86_06565"
                     /gene_synonym="purI"
     CDS             complement(3950..7870)
                     /gene="purL"
                     /locus_tag="PLB86_06565"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRC64834.1"
                     /translation="MGASPAPVLTSFEGGRALSDFRAVGLLRRLRDRVPTITGVTARH
                     VHWVASATPLDADTHTRLAQLLNYGDPYAAPAPDAGGAGDAPVTVLVVAPRLGTVSPW
                     ASKATDIAHNCGFEIRRVERVTEFRLEGVALAGDDLAAAAEVLHDRMTESVLLGHDSA
                     AALFAEREAEPLAHVAVLERGREALVEANATYGLALSDDEVDYLVEAFRGLGRDPSDV
                     ELMMFAQANSEHCRHKIFNARFVVDGEVQDRSLFQMIRNTESVSPRHTVVAYSDNASV
                     MEGGVVPRWLPTEQSEPSRYAAHEDTVHVLMKVETHNHPTAISPFPGAATGAGGEIRD
                     EGATGRGSRPKAGLTGFAVSNLHLPGTDEPWERDPIGRPGHLATPLEIMLDGPIGGAA
                     FNNEFGRPGLGGFFRVYEQTVAGVRRGYHKPIMSAGGLGTISASQTHKVAFPAGTLLV
                     QLGGPGMRIGMGGGAASSMAAGANAAHLDFDSVQRGNPEIERRAQEVVNHCWALGEDN
                     PILAIHDVGAGGLSNAFPELVDGAGLGAHFDLRAIPLEETGLAPKEAWCNESQERYVV
                     ALAPESLALFAQLCERERCPFAVVGVASDDGMLVLADGPDGERAIDMPMEVLLGKPPQ
                     MQRDVERVARTEPELSLEGIDLGVAAFDVLRHPTVASKRFLVTIGDRTVGGLSHRDQM
                     VGPWQVPVGDVAVTLAAYDGFAGEAMASGERMPLAAIDAPASGRMAVGEALTNLLAAP
                     AELERIKLSCNWMAACGEPGEDAALFDTVAAVAMELCPALGVGVPVGKDSLSMRTTWT
                     DESGQGRKVVAPVSLVVTAFAALPDVRGTLTPQLRGGGSDLVLVDLGAGQNRLGGSIL
                     GQVRGGFGGAVPDLDDPARMRALYAAMADLRERDLVLAYHDRSDGGLWATVCEMAFAG
                     RVGVQLGLPAGLSEADVVRALFAEELGVVLEVPRASLGDVVALLREHGLGDCVQVVGR
                     TMKTSAVAVTRGDEVLLHKEIHELQQAWDDVSWRIAALRDNPECADEEHAAAGHPDDA
                     GLQVALSFDAAVDVAAPYVSLGTRPKVAILREQGVNSHVETSYAFDRAGFDTYDVHMT
                     DLQSGRADLAAYQGFVACGGFSYGDTLGAGEGWARSVLFNPALAQAFHEFFHRPDTFA
                     LGICNGCQMMAALADHIPGAEAWPRFTRNRSEQFEGRLSLVEVLDSPSIFFSGMAGSR
                     IPIAVAHGEGLADFSQRGDAAAVHEAMRFVDGAGRPATAYPANPNGSPGGLTAVTTPD
                     GRFTVLMPHPERVLRNAQMSWTSGSVDEMSPWLRMFRNARAWVG"
     gene            complement(7982..9058)
                     /locus_tag="PLB86_06570"
     CDS             complement(7982..9058)
                     /locus_tag="PLB86_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC64835.1"
                     /translation="MTDVAGQGVTLPAPRTPDPQQAPPLRWGLLGAGWIADKFVTSLR
                     AHTTQIPYAVWAPRPLAAAAFAQAHGIPHVHSSVADLLAAPDVDVVYVAGPHHTHHEL
                     TLAAIAAGKPVVVEKPLALNAAQAREIAAAASVAGVFVMEALWSVCLPKFDVVRQVLD
                     AGVLGRIHTVTADIGEYFDRDHRILRRDLAGGPVLDLGTYPVAFATWILGAPSDVRAL
                     AQPDPSGVLSQTAAILRYDEGDHAGALALVHASLATATPTTGEISGELGTLSLDPPFY
                     QPGGVTLRLRDGRAARYDEPRIAHEGLYFEAVEAARCIAAGLTQSPLRPLADSIAMLA
                     VMDEVRRQTGDVFDMEAGGIPDPA"
     gene            complement(9055..10050)
                     /locus_tag="PLB86_06575"
     CDS             complement(9055..10050)
                     /locus_tag="PLB86_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC64836.1"
                     /translation="MSTTAAAETRTKFDRGAFVRQYAIVGILVLFVLGLSLATGGKFL
                     QAQNLLNVVTQVAAIGIIAIGMTFVIITLGIDLSVGSLLAVAAVVATSLAQIPSDTAK
                     FPNMSFSLIIPIVAGLAAGAIGGFTNGFLISAFKIAPFIATLGMMSAARGLALIYSDG
                     RPISRLDPGFNFLGQGSIIGIPVPIIIFAVVAVAAHVVLNYTRFGRHVYAIGGNEQAA
                     RVSGINIKRATIIIYTLCGLLAGIAGIISAGRIGSGNPQLGTGIELDAITAAVIGGTS
                     FKGGIGTIWGTVIGALIIQSLNTGLVLLGMNDFVQMVVKGAIIIIAVILDERKNR"
     gene            complement(10062..11585)
                     /locus_tag="PLB86_06580"
     CDS             complement(10062..11585)
                     /locus_tag="PLB86_06580"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRC64837.1"
                     /translation="MSDEFLLQMQDVSKTFPGVRALAGVNLEVRPGTVHALMGENGAG
                     KSTLMKILIGMYQADPGATIILGGEKVDIPDTAAGLGLGISMIHQELSPVPEMTVAEN
                     IFLGREPVARFGLVSRKQMVSSCRELFQRWEIDIDPRARMRTLSIAQTQMVEIAKAIS
                     FDSRIIIMDEPTSAITEREVEHLHRMIRQLKSTGASIIYITHKMDEVFNISDYVTVFR
                     DGQLVKTLPASELDRDKLITLMVGRELTEMFPKEFVEIGEVVLSVRNLHRGSVVKDVS
                     FDLRRGEILGFAGLMGAGRTEVLETLFGVVAAESGEIVVDGKSVRIRQPKDAIDAGLA
                     LLTEDRKLTGIMGVLSVRDNMIMAALPRYSPGGMLQKALIEEVCQDQRKALALKTPSL
                     NQHIQNLSGGNQQKVLISRWLLTLPEILMIDEPTRGIDVGAKSEIHRLMSALAKTGKA
                     IIMVSSEMPEVLGMSDRVVVMHDGKVSGIVDRADATQERIMQLATGPAGVTGPADAA"
     gene            complement(11719..12753)
                     /locus_tag="PLB86_06585"
     CDS             complement(11719..12753)
                     /locus_tag="PLB86_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRC64838.1"
                     /translation="MAISHGLKVGALLLATTSLFAACSSTAGTTAATTAAATKAAAAT
                     TTTPAAAGSSAVVKPKDGKALKIGVTFPILDQFLQKVADGISARAAEAGVEVNIQASQ
                     EKTDVQLAQVETFIANKVDAIIVVPVDTDAAGPMTAKAQAAGIPLIYVNRKPADLPAG
                     VPYVGSDSLVSGNLEMTQLAKLANGKGNVVILQGDPSNEAAVLRTKGCVDVVAKNPDM
                     KVIKTQAGNWQREKGQSIMETWLQSGDQIDVVCANNDEMALGAINALKAAGKLDKTIV
                     GGVDATGDALAAMKAGELKVTVFQDAKGQGAGGVDAAIKLYNKDTVPAYVDVPYVLVT
                     PENMAQFAGK"
BASE COUNT         1969 a         4569 c         4429 g         2004 t
ORIGIN      
        1 cgcggtcgcg agcgcctccc ggggggccga cgcgaaccct gccgcacggc atacctccag
       61 ggcgatgagg ccctcggcgg gcgggatgcg gcggcggggt ggcaccccca catggtgtca
      121 ccgcgcgcag acacgggagg atggagccat gcgcgcgacc accctgcacg gggccttcga
      181 catccgggtg tccgacctgc ccgaccccaa gctccaccgg ccgaccgacg ccctcgtgcg
      241 ggtgacggcc tcctgcgtgt gcgggtcgga cctgtggccc taccgcggga tcaacgcgag
      301 caagccaggg cagcggatcg gccacgagtt cgtcggcatc gtcgaggagg tcggctcgga
      361 ggtcgccact gtgcgccccg gcgacttcgt catcgccccc ttcgcgtggt cggacaacac
      421 ctgtcgggta tgccgtgtcg gcatccagac ctcgtgcgag aacggcggtt ggtggggagg
      481 ccgcgaccgc gagggcttgc cggtcgacgc cgggcagggc gagttcgtgc gggtgccgct
      541 ggccgatggc accctcgttg cgacccccga ggtgccctcg gccgacctcg tgcccgacct
      601 gctcaccctc tcggacgtga tgggcactgg ctggcactgc gcgctcagcg cgggcgtgcg
      661 accgggcgac accgtggccg tcgtcggtga cggcgcggtc ggattgtgcg cactgctgtc
      721 ggcccggttg cagggcgcga cgcgaatcat cgcgatgtcg cgtcacgagg cccgttcggc
      781 gatcgcggtg gagtttgggg cgaccgaggt cgtggctgag cggggcgagg ccggcgtggc
      841 tcgggtccgc gacctcacgg acggcttcgg tgtcgacgcg gccctggagt gtgtgggcac
      901 cgccgagtcg atgcgcaccg ccctggatct cgtgcggcct ggtggccgga tcggcttcgt
      961 cggcgtcccc cacgacgtca cggtgccgat cgaggacctc ttcgcccgca acgtccacat
     1021 cggcggtggc gtggcgcccg tgcgcgaata tctgcccgcg ttgcgcgacc tcgtcctcgc
     1081 tggcgagatc cacccgggac gcgtcttcga ccttacggtc cccctcgcgg acgtcgccga
     1141 ggcgtatgcc gcgatggacc agcgccgcgc caccaaggcg atgctcctgc catgagcaac
     1201 cacggaggcg agatcgccgg gaccctcatc ggcctgttct gggtgctctt ggggtggtgg
     1261 atgatcgcgg tcggtcgacg ccgcggccgc gtcggcggcg attgggtgcc ggcggtcggg
     1321 accatcgtcg acaagagcgg gggcaccgag ggtctgttcc tgcgcaaccc gcacatccgg
     1381 tatgccgccc ccgacggctc cgagcacatc gtcgccagcc gctcgcacgg cgacctttgg
     1441 gagcccggcc agtcggtcga catcctcgtc gaccccaccg aaccgggtcg cgcggctttg
     1501 ctcacccacg ccgctcgggc gacgccctac gtcgtcatcg ggtggttcat catcgtcgtc
     1561 ggtctgctca cgctcatcgc gtcgctgatg ctcgctgtct gggtgccgcc gccatcgacg
     1621 tgactcggct gactcggctg actcggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnntga ctcggctgac tcggctgact cggcggatgc cgcaacggcg aggcgcgacc
     1801 ccgtgactgg atcagccgaa gggccatcgt ccgagtcggt gcggttcgag ccggacggcc
     1861 acggcgggtg gacggtcatg ctcgacggcc agccccagtc ccatgtcgac ctcgccgacc
     1921 ccgggctgat caccttcgag tacgtccaac acttcgccct cgccatcggc gcgtgctcgc
     1981 ccacggcccc ggcgccgctg cgggtcaccc acgtcggtgg cgccgggctc acgctggctc
     2041 gctgggtgca gcacacccgg ccggtctcgc cgcagatcgt cctggagccc gacgccgcac
     2101 tcaccgcggc cgtccgcgag cggttgccgt tgcctcgcgg gcaccggatc cgggtgcgtc
     2161 ccctcgacgg ccgcaccggg gttcgggggc tggcgacagc gagtgccgac gtcctcgtcc
     2221 tcgatgcgta tgccgcgggc cgagtcccgg ccgagctcgc cactccggag ttcctcgccg
     2281 acgtggctcg agtgctggcc cctggtgggt tgctgctcgc caacctggcc gacgagcccg
     2341 gattgcgttg ggcggcaagg ttcctcgcga cgctggcctc agttgagggg ttcggtgggc
     2401 cagcggtgtt ggcggtgttg gcgacccacg acgtcctcaa ggggcgccgt ttcggcaacc
     2461 tcgtcgcggt ggcatcccgg ggagccgacg acctggccaa tcctgcaccc actgatgctg
     2521 gacccgctcg ttcggactcc gcgaggctct cacccaccga cgccgaagcc aatcgctcgg
     2581 gctccgcgag gctctcaccc accgacgccg aagccaatcg accagactcc tccggcctat
     2641 cgcggaccgg ggatggccag ccccgcctgg acctcgccgc gctgcgtcgg gccggtgcga
     2701 gcgcgccgat cccgaccggc atacggtccg ggcctgaggt gacccgactc atcgcagggg
     2761 cgcgcccatt cgccgacgcg gatgcgcaac cctcacctgt cccgcccgac cccggggcct
     2821 ggcgcgtccg ctgacgctga tccgcctcgc ccgcgcctcc cccctccggc cgggaaactc
     2881 gacactcggt gagttccctc cgagcaccag cccgnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnccccct ccggccggga aactcgacac tcggtgagtt ccctccgagc
     3061 accagcccga ccccggccgg aaagtcgaca ctcgctgact tgcctgccca ccctgcctgc
     3121 cttcgtgtca aaaggtcgac gatctttcgc caggtcgacc gaatttcgtc gagcaacgaa
     3181 aaggtcgtcg acctttcgaa cggcgatgtg gcgcccggcc caagccatcc atccagccag
     3241 tcggccagcg cagggttagc tagaccaggt cgcagatcgc ggcgaccaac gccgacatgt
     3301 cgtcgatgac gacgtcggca tcaaccaact gcccccgcga ggtgacgctg cccgcgaagc
     3361 cgacgcacgg cataccggcg gctcgtgcgg cggccacccc gtgcggcgag tcttcgacga
     3421 cgacgcaccg ctcgggcgcg acgcccaact gcgcgcccgc ccagaggaag aggtcgggcg
     3481 ccggcttccc gcgcgccacg tcggcggcgg tgaagacggt cgcctcggtg aaccactgcg
     3541 ccagcccgca ccgggcgagc ttgcgccacg tcgtcgcgcg ctcgccgctc gtcgcgacac
     3601 acaccgccca cccgtcggcc acgaggtccg cgatcgccgc gcctaccccg ggcacggcct
     3661 ccagttgcgt ctcgtatgcc gcgtgatagg cacttcgcca gcgcagcacc cactcggcgg
     3721 gcacgggccg gccgatgacc gactcgatat cggccgtcgc gtcggcctcc gacagcccga
     3781 cgtgcctggc aatgacttcc tcgcgcgtga tcgcccagcc caactcaccg atcgcttcca
     3841 cgtcgagcgc gacgttgagc cgttcgctgt cgaccagcac gccgtcgcag tcgaagacga
     3901 ccagccgccc cctgcccgac acgcccacgc ccgacacgct catcggcgct cagcccaccc
     3961 aagccctcgc gttgcggaac atccgcagcc acgggctcat ctcgtcgacc gagccgctcg
     4021 tccacgacat ctgcgcgttg cgtagcaccc gctccgggtg cggcatgagc accgtgaagc
     4081 gcccgtccgg ggtcgtcacg gccgtcagcc caccagggct gccgttggga ttcgcggggt
     4141 atgccgtcgc cggcctccca gccccgtcga cgaaccgcat cgcctcgtgc accgccgccg
     4201 catcgccccg ctgcgagaag tcggccagcc cctcaccatg agcgactgcg atcgggatgc
     4261 ggctgccggc catccccgag aagaagatcg acgggctgtc gagcacctcg acgagtgaca
     4321 gccgcccctc gaactgctca ctccggttcc gggtgaaccg cggccacgct tccgctcccg
     4381 ggatgtgatc ggcaagcgca gccatcatct ggcagccgtt gcagatcccc aacgcgaagg
     4441 tgtccggccg gtggaagaac tcgtggaacg cctgcgcgag agcgggattg aacagcaccg
     4501 accgcgccca gccctcgccc gcccccaacg tgtcgccgta ggagaagccg ccacacgcga
     4561 cgaacccttg gtatgccgcg aggtcggctc gcccgctctg caggtcggtc atgtggacgt
     4621 cgtaggtgtc gaagcccgcc cggtcgaagg cgtagctcgt ctcgacgtgc gagttgacgc
     4681 cctgctcacg caggatcgcg accttgggcc gggtgccgag cgacacgtag ggcgctgcca
     4741 cgtcaacagc agcatcgaag ctcaaggcca cctgaagtcc agcgtcgtcc gggtgaccgg
     4801 cagccgcgtg ctcctcatcg gcgcattcgg ggttgtcgcg cagcgcggcg atccgccacg
     4861 agacgtcgtc ccaggcttgc tggagctcgt gaatctcctt gtgcagcaac acttcatcgc
     4921 cccgcgtcac cgcaacggcg gacgtcttca tagtgcgacc cacgacctgc acgcagtcgc
     4981 cgagcccgtg ctcgcgcagc agggcgacga cgtcgcccaa cgaagccctc ggcacttcga
     5041 gcacaacacc gagttcctcg gcgaagaggg cgcgcacgac atcggcttcc gacaggccag
     5101 ccggcagccc gagttgcaca ccgacccgac cggcaaacgc catctcgcac accgtcgccc
     5161 acaggccgcc gtcggaacgg tcgtgatagg ccaagacgag atcgcgctcg cgcaggtcgg
     5221 ccatcgcggc atacaaggcg cgcatccggg ccgggtcgtc gaggtcaggg accgctcccc
     5281 cgaagccgcc gcgcacctgg ccgaggatcg acccgccgag acggttctgg cccgcgccga
     5341 ggtcgacgag cacgaggtcc gaacccccgc ctcgcaactg cggcgtgagc gtgccgcgca
     5401 cgtcgggcag cgccgcgaac gccgtgacga ccaggctgac cggcgcgacg accttgcgcc
     5461 cctgccccga ctcgtcggtc cacgtcgtgc gcatcgacag cgagtccttg ccgactggca
     5521 cgccgacccc caacgcgggg catagctcca tcgccaccgc tgccaccgtg tcgaacagcg
     5581 ccgcgtcttc gccgggctct ccgcaggccg ccatccagtt gcaggacaac ttgattcgct
     5641 caagctcggc gggcgcagcc agcaggttgg tcagcgcctc gccgaccgcc atccgcccgg
     5701 aggcgggggc gtcgatcgcc gcaaggggca tccgctcgcc gctggccatc gcctcgcccg
     5761 caaagccgtc gtatgccgcg agcgtcaccg cgacgtcgcc cacgggcacc tgccacggcc
     5821 ccaccatctg gtcgcggtgg ctcaaccccc cgacggtccg gtcgccgatg gtcacaagga
     5881 aacgcttgct ggccaccgtc gggtggcgca ggacgtcgaa agcagccacc ccgaggtcga
     5941 tcccctcaag gctcaactcg ggctcggtgc gagccactcg ctcgacgtcg cgctgcatct
     6001 gcggcggctt gccgagcaga acctccatcg gcatgtcgat ggcccgctcg ccgtcggggc
     6061 cgtccgccag gacgagcatc ccgtcgtcac tggccactcc gacgacggcg aaggggcacc
     6121 gctcgcgttc gcacaactgg gcgaagagtg ccagcgactc cggggccagg gccacgacat
     6181 agcgctcctg actctcgttg caccacgcct ccttcggcgc aagcccggtc tcctccaacg
     6241 gaattgcccg cagatcgaag tgcgcaccca gacccgcacc gtcgaccaac tcggggaagg
     6301 cgttggacag cccgcccgca ccgacgtcgt ggatcgccag gatcgggttg tcctcgccga
     6361 gcgcccaaca gtggttgacg acctcctggg cccgccgctc gatctcgggg ttgccgcgct
     6421 gcaccgagtc gaagtcgagg tgcgcagcgt tggcaccggc cgccatcgac gacgccgcgc
     6481 cgccacccat gccgatccgc atccccggcc caccgagttg cacgagcagc gtcccggccg
     6541 ggaaggccac cttgtgcgtc tgcgacgctg agatcgtccc gaggccaccg gcgctcatga
     6601 tcggcttgtg gtagccgcgg cgcacgccgg ccaccgtctg ctcataaacc cggaagaagc
     6661 cgcccagccc cggccgcccg aactcgttgt tgaatgccgc gccgccaatc ggcccgtcga
     6721 gcatgatctc cagcggcgtc gccaagtgcc ccggccgacc gatcgggtcg cgctcccacg
     6781 gctcgtcggt gccgggcagg tgcaggttcg acacggcgaa gccggtgagc ccggccttgg
     6841 gtcgcgaccc ccgcccggtc gcgccctcgt cgcggatctc cccgcccgcg ccggtcgccg
     6901 caccagggaa gggcgagatg gccgtcgggt ggttgtgcgt ctcgaccttc atgaggacgt
     6961 gcacggtgtc ctcgtgcgcg gcataccggc tgggctcgct ctgctcggtc gggagccagc
     7021 gtgggacgac cccgccctcc atcaccgagg cgttgtcgga gtaggcgacc acggtatgcc
     7081 gcgggctcac cgactcggtg ttacggatca tctggaagag cgagcggtcc tgcacctcgc
     7141 cgtcgacgac gaaccgggcg ttaaagatct tgtgccgaca gtgctcgctg ttggcctggg
     7201 cgaacatcat caactcgacg tcgctggggt cgcgcccgag cccgcggaac gcctcgacga
     7261 ggtagtcgac ctcgtcgtcg gacagggcca gcccgtaagt cgcgttggcc tcgacgagcg
     7321 cctcgcgccc ccgctcgagg acggcgacgt gcgccagcgg ctcagcctca cgctcggcga
     7381 atagcgctgc ggcactgtcg tgtccgagca gcacgctctc ggtcatccgg tcgtgcaaca
     7441 cctccgcagc ggcggccagg tcgtcgccag caagggcgac accctccagc cggaactcgg
     7501 tcacccgctc gacccggcgg atctcgaacc cgcagttgtg ggcgatgtcg gtcgccttgg
     7561 acgcccacgg cgagaccgtc ccgagacggg gcgcgacgac gaggacggtg accggggcgt
     7621 cgcccgcacc gccggcgtcg ggggccgggg cggcatacgg gtcgccgtag ttgagcaact
     7681 gcgcgaggcg cgtgtgcgtg tcggcgtcga gaggagtcgc actggcgacc caatggacgt
     7741 gtcgggcggt caccccggtg atggtgggga cccggtcgcg caggcgccgc agcagcccca
     7801 ccgcccggaa gtccgagagg gcgcgtccgc cctcgaagct cgtgagcacc ggggcgggcg
     7861 aggctcccat gacaggcgga ctcctcaaca aagggtgggc gcgccgggtc ggcgacggct
     7921 cacgcagagc ctaccgggct gacggggcgg ctcccccggg cgtctcggcg gctacgtgcg
     7981 gtcaggcggg atcgggtatg ccgcccgcct ccatgtcgaa cacgtcgccg gtctggcggc
     8041 gcacctcgtc catgacggcg agcatggcga tcgagtcggc cagcggccgc agcggcgact
     8101 gcgtcagccc agccgcgatg catcgggcag cctcgacggc ctcgaagtac agcccctcgt
     8161 gggcgatccg gggctcgtcg tatcgagcgg cgcggccatc ccgcagccgg agggtcaccc
     8221 cgcccggctg atagaagggc gggtcgagcg agagcgtgcc caactcgccg ctgatctcac
     8281 cggtggtggg cgtcgcggtc gccagcgatg cgtgcacgag ggcgagcgca ccggcgtggt
     8341 cgccctcgtc ataccgcagg atcgcggccg tctggctgag gactccgctg ggatcgggct
     8401 gcgcgagggc gcgaacatcg ctcggcgcgc cgaggatcca cgtggcgaag gcgaccggat
     8461 aggtccccag gtcgaggacc gggccgccgg cgaggtcacg ccgcaggatc cgatggtcgc
     8521 ggtcgaagta ctccccgatg tcggccgtga cggtgtggat acgaccgagg acgccggcgt
     8581 cgagcacctg ccggacgacg tcgaacttgg gcaggcacac gctccagagc gcctccatga
     8641 cgaaaacgcc cgcgaccgag gcagcagcgg cgatctcgcg agcctgggcg gcattgagcg
     8701 ccagcggctt ctcgacgacg accggcttgc ctgcggcgat ggccgccagc gtgagctcgt
     8761 gatgcgtgtg gtggggtccg gccacataga ccacatcgac atcgggggcg gccaacaggt
     8821 ccgcgacgct cgagtggaca tggggtatgc cgtgcgcctg cgcgaacgcg gcggcggcga
     8881 gcgggcgcgg tgcccacacg gcatacggga tctgcgtggt gtgcgcgcga agggacgtga
     8941 cgaacttgtc ggcgatccac cccgcgccca gcaggcccca ccgaagcggt ggggcctgct
     9001 gggggtcggg cgtgcgcggc gccggcagcg tcacaccctg gccggcgacg tcggtcaccg
     9061 gttcttacgc tcgtcgagga tgacggcgat gatgatgatg gcgcccttga cgaccatctg
     9121 gacgaagtcg ttcatgccga gcagcacgag gccggtgttg aggctctgga tgatcagcgc
     9181 gccgatcacc gtgccccaga tcgtgccgat gccgcccttg aagctcgtgc caccgatgac
     9241 cgccgcggtg atcgcgtcga gctcgatgcc ggtgccgagc tgcgggttgc ccgagccgat
     9301 gcggccggcc gagatgatgc cggcgatccc ggcgagcaga ccacagaggg tgtagatgat
     9361 gatcgtcgcg cgcttgatgt tgatgcccga gacacgggct gcctgctcgt tgccgccgat
     9421 ggcgtagacg tgccggccga agcgcgtgta gttgagcacg acgtgagccg caacggcgac
     9481 cacggcgaag atgatgatcg ggacggggat gccgatgatc gagccctggc cgaggaagtt
     9541 gaagcccggg tccagacgcg agatcggtcg gccgtcgctg tagatgaggg cgaggccgcg
     9601 ggccgcgctc atcatgccga gggtggcgat gaacggggcg atcttgaacg ccgagatgag
     9661 gaagccgttg gtgaacccgc cgatcgctcc ggccgccaga cctgcgacga tcggaatgat
     9721 gagcgagaac gacatattcg ggaatttggc tgtgtccgaa ggtatctgag ccaaactcgt
     9781 cgcgacgacc gccgctaccg ccagcaggga cccgacggac aggtcgatgc ccagggtgat
     9841 gatgacgaag gtcatgccga tggcgataat cccgatcgcc gcgacctgcg tcacgacgtt
     9901 gagcaggttc tgcgcctgca ggaacttgcc gccggtggcg agggacaggc cgaggacgaa
     9961 gagcacgagg atgccgacga tggcgtactg ccggacgaag gcaccgcggt cgaacttggt
    10021 cctggtttca gctgctgctg tggtggacat cgagttacct ctcaggctgc gtcagcgggg
    10081 ccggtgacgc ccgcgggtcc ggtggcgagc tgcatgatgc gctcctgggt ggcgtcggcc
    10141 cggtcgacga tgccgctgac cttgccgtca tgcatgacga cgacgcggtc gctcatgcca
    10201 agcacctcgg gcatctccga ggagaccatg atgatggcct tgccggtctt ggccagggcc
    10261 gacatgaggc ggtggatctc ggacttggca cccacgtcga tgccacgggt cggttcgtcg
    10321 atcatgagga tctccggcag cgtgaggagc catcgcgaga tgagcacctt ctgctgattg
    10381 ccacccgaca ggttctggat gtgctggttg agcgaggggg tcttgagggc tagggccttg
    10441 cgctggtcct ggcagacctc ctcgatcagg gccttctgca gcatgccgcc ggggctatat
    10501 ctcggcaggg cggccatgat catgttgtcg cggaccgaca gcacgcccat gatcccggtg
    10561 agtttgcggt cctcggtgag caaggccagg ccggcgtcga tcgcgtcctt aggctgacgg
    10621 atccggacgg acttgccgtc cacgacgatc tcgccgctct cggccgcgac caccccgaag
    10681 agggtctcaa ggacttcggt ccgaccggcg cccatgagcc cggcgaagcc gaggatctcg
    10741 ccgcgacgca ggtcgaagct cacgtccttg acgaccgaac cacggtgcag gttgcgcacc
    10801 gagaggacga cctcgccgat ctcgacgaac tccttgggga acatctcggt gagctcgcgc
    10861 cccaccatga gggtgatgag cttgtcgcgg tccaactccg acgcgggcag ggtcttgacg
    10921 agctggccgt cgcggaagac cgtgacgtag tcggagatgt tgaagacctc gtccatcttg
    10981 tgcgtgatgt agatgatcga cgcaccggtg gacttgagct ggcggatcat ccggtggagg
    11041 tgctcgacct cccgctcggt gatggccgag gtgggctcgt ccatgatgat gatccgcgag
    11101 tcgaagctga tcgccttggc aatctcgacc atttgggtct gggctatgga cagggtgcgc
    11161 atccgggccc ggggatcgat gtcgatctcc cagcgctgga acagctcacg acacgaactg
    11221 accatctgct tgcggctcac caggccgaac cgggccacgg gctcgcggcc gaggaagatg
    11281 ttctccgcca cggtcatctc cgggacgggc gacagctcct ggtggatcat cgaaataccc
    11341 agacccaggc cggcggccgt gtcggggatg tcgaccttct cgccaccgag gatgatcgtg
    11401 gcccccgggt cggcttggta catgccgatg aggatcttca tcaaggtgga tttgcccgcg
    11461 ccgttctcgc ccatgagggc gtggacggtt ccgggtcgaa cctcgaggtt gacgcccgcg
    11521 agcgccctca cccctgggaa tgtcttggac acgtcctgca tctgcaggag gaactcatcg
    11581 ctcacggtta cctcgccctg atcgcggccg cgcctggcgt gcctgggact ggtggctgtg
    11641 ggtttgcggt gggcgggcga ctcgtcgccc gcccaccgcg ggtcactcac tgaccggcaa
    11701 aactactgac cggcaaactc acttaccggc gaactgtgcc atgttctccg gggtcacgag
    11761 gacgtaggga acgtcgacgt aggccggcac ggtgtccttg ttgtagagct tgatcgcggc
    11821 gtcaacgcca ccggcgccct ggcccttggc gtcctggaac accgtcacct tgagctcgcc
    11881 cgccttcatg gcagccagcg cgtcacccgt cgcgtccacc ccaccgacga tggtcttgtc
    11941 gagcttgccc gccgccttga gggcgttgat cgcaccgagc gccatctcgt cgttgttcgc
    12001 acacacgacg tcgatctggt cgccggactg cagccaggtc tccatgatcg actggccctt
    12061 ctcgcgctgc cagttgccgg cctgggtctt gatgaccttc atgtccgggt tcttggcgac
    12121 gacgtccacg cagcccttgg tccgcaggac ggcggcctcg ttggacgggt caccctggag
    12181 gatgacgacg ttgcccttgc cgttggccaa cttggccaac tgggtcatct ccaggttgcc
    12241 ggagacgagc gagtcggaac cgacgtaggg gacgccggcc ggcaggtcag cgggcttgcg
    12301 gttgacgtag atcaggggga tgccggcggc ctgggccttg gccgtcatcg ggccggccgc
    12361 gtcggtgtcg accgggacga cgatgatcgc gtcgaccttg ttggcgatga aggtctcgac
    12421 ctgggccaac tggacgtcgg tcttctcttg cgaagcctgg atgttgacct cgacgccggc
    12481 ctccgccgcg cgggcgctga tgccgtcggc gaccttctgg aggaactggt cgaggatcgg
    12541 gaaggtcacg ccgatcttga gcgccttccc gtccttcggc ttgaccacgg cggagctgcc
    12601 cgcggcagca ggagtcgtgg tggtggcggc agcggccttg gtcgcggcgg ctgtcgtcgc
    12661 ggcggtcgtt ccggccgtgg aagagcaagc ggcgaacagg ctggtcgtgg cgagcagaag
    12721 tgcccccacc ttgagtccgt gcgagatagc catggttcct ctctctgtgg gtgggacagg
    12781 gcccgcaggg cctgcggcta gcccaaacgg gtgccggtga gccacccgtc ctaccggcta
    12841 cgtgtcgcag ctggtaagtc agacgctatt gccgccgtaa cctcctgtca atacatacga
    12901 acaaagatgt tacaaactag tgacccggtt cggcaggccc ctgaggtatg ggccgccctg
    12961 accttaggac gaggcaatac cgttgcaccc caatgatttt cggccgcagt ggtcccgccg
    13021 gccccctcac tgccagccct gtgcgccccc ggagcccgtg gggtggagga ccccttcgac
    13081 cattgatctg tcaccgcgta tgtcctgaca accgtgacaa cagtcgaggc ccgaccgaga
    13141 cgggtctcgg tcgggcctca cctactgacg c
//
LOCUS       DAORPW010000059        13135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_973_length_13135_cov_731.095183, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000059 DAORPW010000000
VERSION     DAORPW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13135
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_973_length_13135_cov_731.095183"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLB86_06590"
     CDS             complement(<1..281)
                     /locus_tag="PLB86_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64839.1"
                     /translation="MSMSLPALAELVADGVSVTQGSRVSIFTNDAAVLPAAAAFVEEV
                     YRRGGLAQIVHADEQFDASAVRWGSESMLRQPPPIEEAAMRWSDVHVSFR"
     gene            complement(307..1815)
                     /locus_tag="PLB86_06595"
     CDS             complement(307..1815)
                     /locus_tag="PLB86_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRC64840.1"
                     /translation="MRSALGADRPLLGVDIGTFEAKGVLATADGTVLARARRRHGLLT
                     PAEGHVEHDPETVWWDGLCAVAAELMARPEARGGVEAVGVSGIGPCVLPVDAQLRPLR
                     SAILYGVDSRASRQIDSLTARLGPGEIFRRSGNSLSSQSAAPKIAWLRDEEPANWEAA
                     RWFLTCQSFLVARLTDEVVIDHATAGYFHPIYDIATQQWDLSDCDDIVEEARLPRLAW
                     SSEIAGTVTAAAHRATGIPEGTRVIVGTADAPAEAVAAGVIDQGSVMAMYGSSGFFIR
                     VGDTLVTDHTLWAAPFVFPGTFVLAAGVATSGTATRWIAEVLDIVGNTDQQTFAALIG
                     LAEQATPGCRGLLALPHFSGERTPFHDPQSRAAFVGLGLSHGRPELARGVIEGVAHAT
                     AAALAAYGKAGAPVRRVAAIGGGTKNDILLRSVSAIAGVTQTVVDSDGAALGDAALAA
                     LAVGAVDSRAQLAAWARIREVVDPDPALSDVLVPDHARFLTLYSALCQWRAR"
     gene            complement(1815..2618)
                     /locus_tag="PLB86_06600"
     CDS             complement(1815..2618)
                     /locus_tag="PLB86_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HRC64841.1"
                     /translation="MSWLSDLFCVTKPVIGMCHLSPLPGDPSYDRGRGVRAIVAHARR
                     EVESLQEGGVDGILISNEFSLPYLTKTEPITAVTMARIIGEINDAIEVPFGVNVLWDA
                     TASIDLAVATDAKFVREVFTGVYAGDFGLWNTNVGQTARHRLAVSGDDVRLLFNIVPE
                     AANYLAGRDLASVTRSTVFNCVPDALCVSGLTAGAPTDNSVLKVVKDNAGDVPVIVNT
                     GVRAETAEPSLRIADAAIVGTALKRDGIFENTVDVARVIELMDVVRSMR"
     gene            complement(2705..3751)
                     /locus_tag="PLB86_06605"
     CDS             complement(2705..3751)
                     /locus_tag="PLB86_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC64842.1"
                     /translation="MNRIAEADGMRRGGAERRDVLTDFVRKIHVNRSVGQMAALLVAA
                     FAIFTILRPAVFLGSYNLQVIALAAPEIGILAIAMMVTMLSGGIDLSVVSIANASAIT
                     ISMTYTAVSSRAGVELANSLALPLVLLGLVVGVAAGAINGLLIAKVGITPILATLGTM
                     QLFNGLELVVTGGRTLYGAPAGLSTLGTTTFLGVPAIFLILVVVAVAIGVLLDRVSLG
                     FRIRYQGANAEASRFSGISPTRTLLATYTIVGLLGGIAGVVFLSRNPSASADYGASYT
                     LLVIVIAVLGGTNPYGGRATVLGVVLATLCLQVVASGFTAMRLSSQQYAMAQGVILIG
                     AMVLDYIRGRGSSR"
     gene            complement(3751..4812)
                     /locus_tag="PLB86_06610"
     CDS             complement(3751..4812)
                     /locus_tag="PLB86_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC64843.1"
                     /translation="MSTPALRQRRSGLLSRWPGRNNEGYLAALLLLVVAVMSAAAPAF
                     FSLHTFFSVIRGATVPWIFAMGVLLVLVSGGIDVSFPAIAVFSAYSAVALTTARGVDA
                     GLLGNFALAMAIGCLLGLVNGILIARFRLPTLIVTLGTAGFFRGAMITFVGATYIAQL
                     PRSLAEQSTRSVISVNTEAGTTFLHVLVVPVLILTVLIALGLKHTMFGRAAYAIGGDP
                     EAARRVGIPVQRTQVVLYVLVGALAAFGGMVFMIQGQAADPQMLIGIELDTIAAVVLG
                     GASIFGGRGSVLGTVLGVLLVQVINNSLVLVGVPTAWQRATVGLLLLLGVGAQAWAGR
                     RPRRAAPAAVKAPAVKAAA"
     gene            complement(4809..6314)
                     /locus_tag="PLB86_06615"
     CDS             complement(4809..6314)
                     /locus_tag="PLB86_06615"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRC64844.1"
                     /translation="MDISPAPRPVLELRDIVKTYGGVHALDTVSFTLLPGEVHCLAGE
                     NGSGKSTLIKIISGVETPDSGVIVVGGERHASMTPASAIAAGVQVIYQDFSLFPNLSV
                     AENIAMPRQRAVGGRFYSARAARPIAQGIVDELGLALDLDADVAALSVADKQLTAICR
                     ALVSDAQIIIMDEPTTALTGAEVTALFALVRRLQSRGVSLIFVSHKLDEVLRVSQRLT
                     VLRNGTWVATGAAGDYDRDAIAGLMTGKDIDTSRVVSAVPADAAIALEVKGLGRPGAF
                     ADVDLVVRCGEIVGLTGLLGSGRGEIAEAIFGVRPATTGTISVGGHPVRIQSIPDAVA
                     AGIGYVPEDRLTQGLFLDDSIADNVVAASVTAFAGRARLLDRARMLAFIQSVASRLRI
                     KAPNLSLAVRSLSGGNAQRVVLAKWLMTNPKVLLLNGPTVGVDVGSKSEILEILRTQA
                     SAGMGVVVISDDIPELYSACHRVLIVQGGRIVCELAGDAVRVETIEQRIAA"
     gene            complement(6444..7493)
                     /locus_tag="PLB86_06620"
     CDS             complement(6444..7493)
                     /locus_tag="PLB86_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009212863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HRC64845.1"
                     /translation="MTRTRLAKPAALLAVGSIALTAGCGSTAGTTSTSASAAAKPVDA
                     KSMTMVTVVKLTGVGWFDRMNQGIKAFGQRTGVDARMDGADTASPEKQIAIIQNLIAQ
                     KPTAITVVPNSPAATESVLKSAREQGIVVVTHEAPGMTNVDADLEAFDNADYGAAIMD
                     AMASCMGKSGKYVQFVGALTAETHLAWVKGAYEKGKSAYPGMTRISDPIESKEDENTA
                     YARAKELLTRDPEIKGFQGSAATDVAGIARAVQETGRAADTCVIGTSIPSIAGKYLTD
                     KSIDQIFFWDPAAAGEAQLALALKIAKGEKITEGTDLGVDGYHKLKKSPTMANVWLGD
                     AQVSVDASTASKYPF"
     gene            7721..8485
                     /locus_tag="PLB86_06625"
     CDS             7721..8485
                     /locus_tag="PLB86_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRC64846.1"
                     /translation="MVRKADILDDLLENGYRSVPDLALAFGVDASTIRRHLARLEKLN
                     LVQRTHGGALPIRHADTPFAVKQSLHTSEKVAIGAAMAARIEDGQTVLLDSGSTTLEV
                     AKRLTARGLTIVTNDLRIGLEVANRQLGHLVMLGGELLPSVYTLHGPATLRHLADIRV
                     SVAVFSADSVMADGVFNSNAHEVELKRKMRDISSRSFLVADSSKFCRESLYKVFDLDN
                     FDAVLTDTGISPKVAGWFTIPVERHDARPAVTVPHL"
     gene            complement(8557..10035)
                     /gene="hflX"
                     /locus_tag="PLB86_06630"
     CDS             complement(8557..10035)
                     /gene="hflX"
                     /locus_tag="PLB86_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRC64847.1"
                     /translation="MTRRTDLLAGKATSLADDDLDPHGWGSAAYPGGPGTDFDGDQLD
                     REERAALRRVAGLSTELEDVTEVEYRQLRLERVVLAAVWTEGTVQDAENSIRELAALA
                     ETAGSTVLDGVIQRRQAPDPATFLGKGKAVELRDIVLAEGADTVICDGELAPGQRRAL
                     EDVVKVKVIDRTALILDIFAQHAQSREGKAQVELAQLQYLLPRLRGWGESMSRQAGGR
                     VAGGEGIGSRGPGETKIELDRRRINTRIAKLKRDIAAMKTGRDTKRHSRRAGHVPSVA
                     IAGYTNAGKSSILNRITGAGVLVENQLFATLDPTVRRAQTSDGREYTLVDTVGFVRAL
                     PHQLVEAFRSTLEEVADADLLLHVVDGSHPDPEGQIAAVREVLAGVKGASDVPEVIVI
                     NKADVADPEVIDRLIRTHKHSIVVSARTGEGLDALLELVADGLPKPDVEVELLLPYDR
                     GDLLSRVHEHGEVLTTEHTETGTRVRAKVHAQALAALAAYRV"
     gene            10096..10698
                     /locus_tag="PLB86_06635"
     CDS             10096..10698
                     /locus_tag="PLB86_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRC64848.1"
                     /translation="MSEHEHYFTATPASPEERRTLTVDLAGRRVTITSAGGIFSPDRV
                     DVGTAVLLAEVPDPPLTGSLLDLGCGWGPVALTLGMLSPAADVYAVDVNERALQLARD
                     NAASLGLNRIQVCRPDEVPADVRFAQIWSNPPIRVGKAVLHDLLRTWLPRLEPGGTAY
                     LVVAKNLGSDSLQRWIATDLGMPCERYVTSRGFRVLTVTG"
     gene            complement(10688..11575)
                     /gene="dapF"
                     /locus_tag="PLB86_06640"
     CDS             complement(10688..11575)
                     /gene="dapF"
                     /locus_tag="PLB86_06640"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRC64849.1"
                     /translation="MSGLAFTKGHGTENDFVLVADPDGRLDLRPEQVRALADRRAGIG
                     ADGVIRVVPTHLADDPDVRAQSGFAPWFMDYRNADGSLSEMCGNGTRVFAAYLVREGL
                     QAPGAFAIATRAGVKAVRTTDTGYAVDLGPARVDESEGAAESGFNAVVHPHGGPELPG
                     LRVDVGNPHTVVVLPPHVALDGLDLTVAPHVAPHPPEGTNVEFVQVVGPGHIAMRVHE
                     RGVGETRSCGTGAVAAATATRWWDGPDDAQHRWTVDVPGGRLTVTFTEAGTAELAGPA
                     VLVADGTWTEGPGSGSVSL"
     gene            complement(11577..12554)
                     /gene="miaA"
                     /locus_tag="PLB86_06645"
     CDS             complement(11577..12554)
                     /gene="miaA"
                     /locus_tag="PLB86_06645"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRC64850.1"
                     /translation="MPTVTTTTSDVPISPLVVAVVGPTATGKSALAVALALALDGEIV
                     NADASALYRGMDIGTAKPTLAERRGIPHHQLDVLEVTQEASVAAYQQGARADVEAIIA
                     RGRLPILAGGSGLYVRAALDHLDIPPTDPAVRARLEAEATADPDGPSHLRSRLAQLDP
                     VAAQRIEPNNVRRIVRALEVIELTGRPFSATMPERRHLLPTVMIGLTLDRASLDARIE
                     ARTRAMWDAGLVAEVEHLLDRGLAQGRTARTALGYAQAIDQLRGALTQTEAIEATAAA
                     TRRFARRQESWFRPDPRITWLPAEAPDLLGRAQEAVKAASQTSAMPENG"
     gene            complement(12526..13134)
                     /gene="def"
                     /locus_tag="PLB86_06650"
     CDS             complement(12526..13134)
                     /gene="def"
                     /locus_tag="PLB86_06650"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRC64851.1"
                     /translation="MAIRPVVIHGEPVLHRVASRVERFDDELAELVQDMYDTMDAAHG
                     VGLAAPQIGVGLRVFVYDMANHDGVPARGVVVNPILAVSKVPSGRPDPDEESEGCLSV
                     PGEHFPLRRAETAKVNGQDLTGAPFEFTATDWFARCMQHEFDHLNGKLYVDRLDDKQT
                     RKARKAIKNNGWGKPGHSWMPGADPDPFGHGDLDADGDDHDL"
BASE COUNT         2093 a         4450 c         4551 g         2041 t
ORIGIN      
        1 cggaaggaca cgtggacgtc cgaccagcgc atggccgctt cctcgatcgg cggcggctgg
       61 cgcagcatcg actcgctgcc ccatcggacg gcggaggcgt cgaactgctc gtcggcgtgg
      121 acgatctggg ccagcccacc ccggcggtag acctcttcga cgaacgccgc ggcggcaggg
      181 agcacagcgg catcgttggt gaagatggag acccgagacc cctgggtcac cgacacgccg
      241 tcggcaacga gttccgctag ggccgggagg ctcatggaca cggggaccgc ctttcgcaca
      301 acgagatcag cgagcacgcc actggcacag cgcgctgtag agagtaagga acctggcgtg
      361 gtccggcacg aggacgtcgc tcagcgccgg gtccgggtcg acgacctcgc ggatgcgggc
      421 ccacgcggcc aactgcgcgc gggagtcgac cgcgccgacc gcgagcgcag ccagggcggc
      481 gtcgcccagc gcggcgccat cggagtcgac gaccgtctgg gtcacgcctg cgatcgccga
      541 cacgctgcgc aggaggatgt cgttcttggt cccgccaccg atggccgcca cccggcgcac
      601 gggcgctccg gccttcccgt atgccgccag cgcggcggcg gtcgcatggg ccactccctc
      661 gatgactccg cgggcgagct ccggccggcc gtgggagagc ccgaggccga cgaaggccgc
      721 tcggctctga gggtcgtgga agggcgtgcg ttcgccgctg aaatgcggaa gggccagcag
      781 gccacgacag ccgggagtgg cctgttcggc aagaccaatc agagcggcga aggtctgttg
      841 gtcagtgttg ccgacaatgt cgaggacctc ggcgatccac cgagtggcgg ttcccgaggt
      901 ggccacgccg gcggcgagga cgaaggtccc cgggaagacg aagggcgcag cccacagggt
      961 gtggtcggtc acgagggtgt cgccgacccg gatgaagaag cccgacgatc catacatggc
     1021 catcaccgaa ccctggtcga tgacaccagc ggccacggct tcggccggcg catcggccgt
     1081 cccgacgatg acgcgagtgc cttccgggat gccggtggca cggtgcgcag ctgcagtcac
     1141 ggtccccgcg atctcgctcg accaggccaa ccggggcaac cgggcttcct cgacgatgtc
     1201 atcgcagtcg ctcaggtccc actgctgcgt ggcgatgtcg tagatcgggt ggaagtagcc
     1261 cgccgtggca tggtcgatga ccacctcgtc ggtcagtcgg gccacgagga aggactggca
     1321 ggtgaggaac caccgcgctg cctcccagtt ggcgggctcc tcgtctcgca gccaggcgat
     1381 cttgggggct gccgactgac tggacagcga gttgccagag cgtcggaaga tctcccccgg
     1441 ccccagccgc gcggtgagtg agtcgatctg gcggctggcc cgcgagtcga cgccgtagag
     1501 gatcgccgag cggagcggcc ggagttgcgc atcgacgggc aggacgcacg gtccgatccc
     1561 gctcactccg accgcttcaa cgccgccccg ggcctcgggt ctggccatca actccgcggc
     1621 cacggcgcac agcccgtccc accacacggt ttcggggtca tgctcgacat ggccctctgc
     1681 gggggtgagc aggccgtgtc tgcggcgggc ccgagccagc accgtcccgt ccgcagtagc
     1741 cagcacgccc ttggcctcga acgtcccgat gtcgaccccc aggagcgggc ggtcggcacc
     1801 gagggcggat cgcatcagcg catgctccgc acgacgtcca tcaactcgat gaccctggcc
     1861 acatccacag tgttctcgaa gatgccatcg cgcttcaagg ccgtcccgac gatcgcggca
     1921 tcggcgatcc gcaggctcgg ctccgcggtc tcggcccgga ctccggtgtt gacgatgacc
     1981 ggaacgtcgc cggcgttgtc cttgaccacc ttgaggactg agttgtccgt cggagcgcct
     2041 gccgtcagtc cggagacgca cagggcatcg ggaacgcagt tgaacaccgt cgagcgggtc
     2101 accgacgcga ggtcgcgccc ggcaaggtag ttggcggcct cgggcacgat gttgaagagc
     2161 aaccggacgt cgtcgccgga cactgccaac cggtggcgcg cggtctgtcc gacgttcgtg
     2221 ttccacagac cgaagtcacc ggcatacacc cccgtgaaca cctcacgcac gaacttggcg
     2281 tcggtcgcga cggcgaggtc gatgctggcg gtcgcgtccc agaggacgtt cacgccgaag
     2341 ggaacctcga tcgcgtcgtt gatctcgccg atgatgcggg ccatggtcac agccgtgatg
     2401 ggctcggtct tggtcaggta gggcaggctg aactcattcg agatgaggat gccgtccacg
     2461 ccaccctcct gaagcgactc cacctcgcgg cgtgcgtggg cgacgattgc ccggacgccg
     2521 cgccccctgt cataggaggg gtccccgggg agcggggaga ggtgacacat gccgatgacc
     2581 ggcttggtca cacagaagag gtcgctgagc caggacacgc tggtactgcc tttcgaaacg
     2641 tagggggtgg tgctggggtg atgggtgagg ttggcgctcg ccgacggtga cgccggatcg
     2701 ggtctcatct cgaggaacca cgacctcgga tgtaatccag gaccatggcg ccgatgagga
     2761 tgacgccctg cgccatggcg tattgctggg agctcaatcg catggccgtg aacccgctgg
     2821 cgacgacctg caggcagagc gtggccagca ccacgccgag gacggtggcc cggccgccgt
     2881 acgggttggt gccgccgagc acggcgatga cgatgacgag cagggtgtag gaggcgccgt
     2941 agtcggcact cgcgctgggg ttgcgcgaca ggaagaccac gccggcgatg ccgcccagca
     3001 gtccgacgat ggtgtaggtc gccaacaggg tccgcgtcgg gctgatcccg gagaaccggc
     3061 tcgcctcggc gttggctccc tggtaacgga tccggaaacc cagcgagacc cggtccagga
     3121 gcacaccgat cgccacggcc accacaacga gaatgaggaa gatcgccggc actccgagga
     3181 acgtcgtcgt ccccagcgtg ctcaaccccg ccggggcccc gtaaagcgtc ctgccgccgg
     3241 tgacgacgag ctcgagcccg ttgaacagtt gcatcgtgcc cagggtggcg aggatggggg
     3301 tgatcccgac cttcgcgatg agcagtccgt tgatcgcgcc cgccgccacc ccgaccacga
     3361 gccccagcag gaccaacgga agagcgagcg agttggccag ctcgaccccg gcccgagagc
     3421 tcacggccgt gtaggtcatc gagatcgtga tcgccgaagc gttcgcgatg gagacgaccg
     3481 acaggtcgat cccgccactg agcatcgtca ccatcatggc gatggcaagg atgccgatct
     3541 cgggggcggc cagggcgatg acctggaggt tgtacgagcc caggaacacg gcagggcgca
     3601 ggatggtgaa gatcgcgaag gcagccacga ggagcgcggc catctgtccg accgagcgat
     3661 tgacatggat cttgcggacg aagtcggtga gcacgtcccg acgctcggcg ccaccacggc
     3721 gcatgccgtc cgcctcggcg atgcggttca tcatgccgct gccttcacgg ccggggcctt
     3781 cactgctgcg ggcgccgcgc gacgcggtct gcgcccggcc cacgcttggg cgccgactcc
     3841 gagcaggagc agcaggccaa ctgtggctcg ttgccaggcg gtcgggaccc cgaccagcac
     3901 gaggctgttg ttgatcacct ggacgagcaa cacaccgagt accgtgccga ggaccgagcc
     3961 gcgtccaccg aagatcgacg cccctccgag gacgacggcc gcgatggtgt ccaactcgat
     4021 gccgatgagc atctgggggt cggcggcctg cccctggatc atgaagacca tcccgccgaa
     4081 ggccgcgagg gcgccgacca gcacgtagag gacgacctgg gtccgctgca ccgggatgcc
     4141 gacccgccgg gccgcctcag ggtcaccccc gatggcgtat gccgcgcggc cgaacatcgt
     4201 gtgtttcagg ccgagcgcga tgagcaccgt gaggatgagc accgggacga cgaggacgtg
     4261 caggaacgtc gtccccgcct ccgtgttcac ggagatcacc gagcgggtgc tctgctcggc
     4321 cagcgaccgc ggcagttggg cgatgtaggt ggctccgacg aaggtgatca tcgcgccgcg
     4381 gaagaagcct gccgtgccca atgtgacgat cagtgtcggc aggcggaacc gcgcgatgag
     4441 gatgccgttc accagcccaa ggaggcagcc gatggccatg gcgagcgcga agtttccgag
     4501 cagtcccgcg tcgacgcctc gcgccgtggt gagggcgacc gcggagtatg ccgagaacac
     4561 ggcgatcgcc gggaaggaga catcgattcc gccggacacc aggaccagca acacgcccat
     4621 ggcgaagatc cacgggacgg tcgcgccacg gatcaccgag aagaaggtgt gcaggctgaa
     4681 gaacgccggt gcggcagcgg acatcacggc cacgacgagc agcaacaggg cggcgaggta
     4741 gccctcgttg ttgcgccccg gccagcggga cagcaacccc gagcgccttt ggcgcagtgc
     4801 cggcgtgctc atgcggcgat ccgctgctcg atggtctcaa cccggacggc atccccggcc
     4861 aactcgcaga cgattcggcc cccctgcacg atgaggaccc gatggcacgc gctgtacaac
     4921 tcggggatgt cgtcggagat gacgacgacg cccatccccg cgctcgcctg ggtccgcagg
     4981 atctcgagga tctcggactt ggagcccacg tcgacgccga cggtggggcc gttgagcagc
     5041 agcaccttgg ggttggtcat caaccacttg gcgagcacga cacgttgggc gttcccgccc
     5101 gagaggctgc gaaccgcgag gctgaggttg ggcgccttga tgcgtagccg gctcgcgaca
     5161 ctctggatga aggcgagcat ccgcgcccgg tccagcagcc tggcccgacc ggcaaatgcg
     5221 gtgaccgagg ccgccacgac gttgtcggcg atggagtcgt cgaggaacaa cccttgggtc
     5281 agtcggtctt ccgggacgta gccgatccct gcggcgaccg cgtccgggat cgattggatg
     5341 cgcaccgggt gaccgccgac ggagatcgtc cccgtggtgg ccgggcgcac cccgaagatg
     5401 gcctcggcga tctcgccgcg ccccgaaccg agcagcccgg tcagaccgac gatctccccg
     5461 caccggacca ccaggtcgac gtcggcgaac gcgcccggtc ggccgagacc cttgacctcc
     5521 agggcgatgg cggcgtcggc cgggacggca gacaccaccc gcgaagtgtc gatgtccttg
     5581 ccggtcatca gcccggcgat cgcatcgcgg tcgtagtccc ccgccgcacc ggtggcgacc
     5641 caggtgccgt tgcgcagcac ggtgaggcgt tgggagaccc ggagcacctc gtcgagcttg
     5701 tggctgacga aaatcagcga cacaccgcgg gactgcaacc ggcgaacgag ggcgaacagc
     5761 gcggtgacct cggctccggt caacgccgtg gtgggctcgt ccatgatgat gatctgggcg
     5821 tcgctgacga gcgcccggca gatggcggtc aattgcttgt cggcaaccga cagggccgcg
     5881 acatcggcat ccagatcaag ggccaggccg agctcgtcga cgatcccctg cgcgatgggg
     5941 cgggctgcgc gggccgaata gaaccggcca cccacagcgc gttggcgcgg catggcgatg
     6001 ttctcggcca cgctgaggtt cggaaagagc gagaagtcct ggtagatgac ttgaaccccg
     6061 gctgcgatgg cgctggccgg ggtcatgctc gcgtgccgct cgccgccgac gacgatcacg
     6121 ccggagtcgg gagtttccac gccgctgatg atcttgatga gcgtcgactt cccgctgccg
     6181 ttctcaccag cgaggcagtg cacctcgccg ggcaggagtg tgaaggacac ggtgtcgagg
     6241 gcgtggacac caccgtaggt cttgacgatg tcgcggagct ccagcacggg ccgtggggcc
     6301 ggtgagatgt ccatggtcgt ccttggttcg gatggagcag cggcctcggt gttgggtcgg
     6361 ggcctccccg gtcagaagcg gtggggggaa accggagagg ccccgacggt tgagcggtct
     6421 gcgcgcttgc gcagccgcag ctcttagaac gggtacttcg aggcggtgct ggcgtcaacg
     6481 gagacctgcg cgtcaccgag ccagacgttg gccatggtgg gcgacttctt gagcttgtgg
     6541 tagccgtcga cacccaggtc tgtgccctcg gtgatcttct cccccttggc gatcttcaag
     6601 gccagcgcga gctgcgcctc accggccgcg gccgggtccc agaagaagat ctggtcgatc
     6661 gacttgtcgg tcagatactt gccggcgatg gaggggatgc tcgtcccgat gacacaggtg
     6721 tcggcggctc ggcccgtctc ctgcaccgcc ctggcgatgc ccgcgacgtc cgtcgcggct
     6781 gacccctgga aacctttgat ctccgggtcg cgggtgagca gttccttcgc cctcgcgtat
     6841 gccgtgttct cgtcttcctt ggattcgatc gggtcgctga tcctggtcat accggggtag
     6901 gcggacttgc ccttctcgta ggcacccttg acccaggcca ggtgggtctc ggccgtcagc
     6961 gccccgacga actggacgta cttcccggac ttgcccatgc acgaggccat cgcatccatg
     7021 atggcggcgc cgtagtcggc gttgtcgaag gcctcaaggt cggcatcgac gttggtcatg
     7081 cccggtgcct cgtgggtgac cacgacgatg ccttgctcac gggcactctt gaggaccgat
     7141 tcggtcgcgg ccggcgagtt cgggacgacg gtgatggccg tcggcttctg ggcgatgagg
     7201 ttctggatga tcgcgatctg cttctcgggg ctcgcggtat cggcaccgtc catccgcgcg
     7261 tccaccccgg tccgctgacc gaaggccttg atgccttgat tcattcggtc gaaccaaccc
     7321 acgccggtga gcttgaccac ggtgaccatc gtcatcgact tcgcgtcgac cggcttcgcc
     7381 gcagcgctgg cgctcgtcga ggtggtccct gcagtgctac cgcagccagc ggtgagcgcg
     7441 atcgacccaa cggcgaggag tgctgccggc ttggcgagcc tggtacgggt catgcggtat
     7501 ctccatgctt cgttcgaacc gccggggctg gcggtcgcca tgacgtgaac gatacgcacg
     7561 tgttcgcgca gtgtcaatac ccttcttggg atcatttgcc cgattacgtt cgtcacagtg
     7621 cctgatatca ggcactcatc tggcgctagg tgggcgaaac gcgctacggt gggcacgtct
     7681 tctccacgag aaggcgcgtg gcacaccacc aaggagcctc atggtccgca aggccgacat
     7741 tctcgatgac ctcctcgaga acggctatcg cagcgtcccc gatcttgcgc tggcgttcgg
     7801 ggtggacgcg tcgaccatcc gccggcatct ggcccggctc gagaagctca acctcgtgca
     7861 gcgcacgcat ggcggcgccc tccccatccg ccacgcggac actccgttcg ccgtcaagca
     7921 gtcgctgcac acctccgaga aggtggcgat cggcgcggcc atggccgccc ggatcgagga
     7981 tggccagacc gtcctgcttg acagcggctc gacgacgctc gaggtcgcca agcgcctcac
     8041 cgcgcgcggc ctaaccatcg tgacgaacga cctgcgcatc ggcctcgagg tcgctaaccg
     8101 acaactcggc cacttggtca tgctcggggg cgagctactc cccagcgtgt atacgctgca
     8161 tgggccggcg acactgcgtc atctggccga catccgcgtc tcggtcgccg tgttcagcgc
     8221 cgactcggtg atggccgacg gggtcttcaa ctccaacgcc cacgaggtcg agctcaaacg
     8281 caagatgcgc gatatctcca gccgatcctt cctcgtcgcc gactcctcca agttctgccg
     8341 ggagtccctc tacaaggtgt tcgacctgga caacttcgac gccgtcctca ccgacaccgg
     8401 gatcagcccg aaggtcgccg gctggttcac gatccctgtc gagcgccacg acgcgcggcc
     8461 cgcggtcacc gtcccgcacc tctgagccat agcccattca ccccgccgac gtgacaccac
     8521 gtcgggggcg ccgcgcggca tacccacggg gcaggatcag acccggtatg ccgccagcgc
     8581 ggccagtgcc tgcgcatgca cttttgcacg aactcgcgta cccgtttccg tgtgctcggt
     8641 ggtgagcact tccccgtgtt cgtgcacccg gctgagcaga tcaccgcggt cgtaaggcag
     8701 cagcaactcg acctcgacat cgggcttggg cagcccgtcg gcgacgagtt ccagcagggc
     8761 atcgagcccc tcgccggttc gggccgacac gacgatcgag tgcttgtggg tgcggatcaa
     8821 ccggtcgatg acctctggat cggcgacgtc ggccttgttg atgacgatga cttcaggcac
     8881 gtcactggca cccttgacgc ccgcgagcac ctcgcgcacg gcggcgatct gaccctcggg
     8941 gtccgggtgc gacccgtcca cgacgtgcag cagcagatcg gcgtcagcga cctcctcgag
     9001 ggtggagcgg aacgcctcga cgagttggtg cggcagcgcc cgaacgaatc cgaccgtgtc
     9061 gacgagcgtg tactcccgac cgtcggacgt ctgggcccgc cggaccgtcg ggtcgagggt
     9121 cgcgaacaac tggttctcga cgagcacgcc ggcaccggtg atccggttga ggatcgagga
     9181 cttaccggcg ttggtgtagc cggcgatcgc cacgctgggg acatgcccgg cccgccgcga
     9241 gtgccgcttg gtgtcgcggc cggtcttcat ggccgcgata tcccgcttga gcttggcgat
     9301 ccgcgtgttg atccggcgcc gatcaagctc gatcttcgtc tcgcccggcc cacgcgaccc
     9361 gatgccctcg ccaccggcga cccggccacc ggcctgacgg ctcatcgact caccccaccc
     9421 acgcaatcgg ggcaggaggt actgcaattg ggcgagctcg acctgggcct tgccctccct
     9481 gctctgggcg tgctgggcga agatgtcgag gatcaacgcg gtgcggtcga tgaccttgac
     9541 cttgacgaca tcctccagcg cgcgccgctg accgggggcc agttcgccgt cgcagatcac
     9601 cgtgtcggcg ccttcggcca ggacgatgtc acgcaactcc acggccttgc ccttgcccag
     9661 gaaggtcgcc ggatcggggg cctggcgacg ctggatcacc ccgtcgagca ccgtcgagcc
     9721 ggcggtctcg gcgagcgcgg ccaactcgcg gatcgagttc tcggcgtcct ggacggtccc
     9781 ctcggtccag acggcggcca gcacgacccg ttcgaggcgc aactggcggt attcgacctc
     9841 ggtgacgtcc tcaagctcgg tcgagagccc cgcaacgcgc cgcagggcgg ctcgctcctc
     9901 gcgatcgagt tggtcaccgt cgaagtcggt gccagggccg ccggggtagg ccgcgctccc
     9961 ccacccgtgc gggtcgagat cgtcatcggc aagcgaggtg gccttgccgg ccagcagatc
    10021 agttcgtcgt gtcatcgtgg gtctaagcct cgcacggcaa acgccgactc gaccaaccgt
    10081 ttaggtttag gggcgatgag cgagcacgag cactacttca ccgcgacgcc ggcctcccca
    10141 gaggagcgac gcaccctcac cgttgacctg gctggccgtc gcgtcacgat cacgagcgcg
    10201 ggaggcatct tttcgcctga ccgcgtcgat gtgggcacgg cggtcctgct cgccgaggtc
    10261 cctgaccctc ccctcacggg atcgctcctg gacctcggct gcgggtgggg accggtcgcc
    10321 ctcacgctcg ggatgctctc ccccgcggcc gacgtctacg ccgtcgacgt caacgagcgg
    10381 gccctgcaac tcgcccgcga caacgcagca tcgttggggc tcaatcggat tcaggtatgc
    10441 cgccctgacg aagttcctgc cgacgtccgg ttcgcacaga tctggtccaa cccgccgatc
    10501 cgcgtgggca aggcggtcct gcacgacctg ctgcgcacct ggctgccgcg gctggaaccc
    10561 ggcgggacgg catacctcgt cgtcgcgaag aacctcggaa gtgactcctt gcagcggtgg
    10621 atcgccaccg acctcggtat gccgtgtgag cgctacgtga cctcgcgcgg cttccgggtg
    10681 ctcacggtca caggctgacc gacccgctcc ccggcccttc ggtccaggtg ccgtcggcga
    10741 caagcaccgc gggaccggca agttcagcgg tcccggcctc ggtgaaggtc accgtgagcc
    10801 ggccaccggg cacgtcgacg gtccaccggt gttgggcatc gtcgggaccg tcccaccacc
    10861 gggtcgccgt ggccgccgcg acggcacccg tgccgcagga gcgggtctcc cccacgcccc
    10921 gctcatggac ccgcatggcg atgtgacctg ggccgacgac ctgcacgaac tcgacgttgg
    10981 tgccctcggg cgggtggggt gccacgtggg gggcgacggt gaggtcaagg ccgtccaacg
    11041 cgacatgcgg cggcaggacg acgacggtgt gcgggttgcc gacatcgaca cgaagcccgg
    11101 gcaactcggg acccccgtgc gggtggacca cggcgttgaa cccgctctcg gcggcccctt
    11161 cggactcgtc aacccgggcg ggacccaggt ccacggcata cccagtgtcg gtggtgcgga
    11221 ctgccttgac gcccgcacgg gtagcgatcg cgaaggcgcc gggcgcctgc aggccctcgc
    11281 ggacgaggta tgccgcgaac acccgcgtcc cgttgccgca catctcgctc agcgatccgt
    11341 cggcgttgcg gtagtccatg aaccacggcg cgaaccccga ctgcgcccgc acgtcggggt
    11401 cgtcggcgag gtgggtcggg acgaccctga tgacgccgtc ggcaccgatg ccggcccggc
    11461 ggtcggcgag ggcgcggacc tgctccgggc gcaggtcgag gcgcccgtca gggtcggcga
    11521 cgaggacgaa gtcgttttcc gtcccgtgtc ccttggtgaa ggccagccca ctcatgtcag
    11581 ccattctcag gcatcgccga cgtctggctc gccgctttga cggcctcctg ggcgcgcccg
    11641 agcaggtcgg gtgcctcggc cggcagccag gtgatgcgcg gatcgggtcg gaaccacgac
    11701 tcctgccgcc gggcgaacct ccgggtggcg gcggcggtcg cctcaatggc ttccgtctgg
    11761 gtgagcgcgc cgcgcagttg gtcgatcgcc tgggcgtaac ccagtgcggt gcgagcggtg
    11821 cggccctggg cgaggccgcg gtcaagcagg tgctccacct cggccaccag cccggcatcc
    11881 cacatcgcgc gggtgcgtgc ctcgatcctc gcatcgaggc tggcgcggtc gagcgtcagc
    11941 ccgatcatga cggtcgggag caggtggcgc cgctcgggca tcgtcgcact gaagggtcgg
    12001 ccggtcagct cgatcacctc caacgcccgg acgatgcgcc ggacgttgtt gggctcgatc
    12061 cgttgcgccg caacgggatc cagctgcgcc aagcgggaac ggaggtggct gggtccgtcg
    12121 gggtcggccg tggcttcggc ctccagccgg gctcggacgg ccgggtcggt cggcgggatg
    12181 tcgaggtggt cgagcgcagc ccgcacatag agccccgacc caccggcgag gatcgggagc
    12241 cggccccgcg cgatgatcgc ctcgacgtcg gcccgggcgc cctgctggta tgccgcgacg
    12301 ctcgcctcct gggtgacctc caggacgtcg agttggtggt gcgggatgcc gcgacgttcg
    12361 gcgagggtcg gtttggcggt gccgatgtcc atcccgcggt agagcgcgct cgcgtcggcg
    12421 ttgacgatct cgccgtcgag ggccagggcc aacgcgaccg ccaaagccga cttcccggtg
    12481 gccgtcggac cgacgacggc cacgacgagc gggctgatcg gcacgtcaga ggtcgtggtc
    12541 gtcaccgtcg gcatcgaggt cgccgtgccc gaacgggtcg gggtcggcgc ccggcatcca
    12601 gctatggccc ggtttgcccc acccgttgtt cttgatggcc ttgcgtgcct tgcgcgtctg
    12661 cttgtcatcg agccggtcga cgtagagctt gccgttgaga tggtcgaact cgtgctgcat
    12721 gcaccgggcg aaccagtcgg tggcggtgaa ctcgaacggc gcccccgtca ggtcctgacc
    12781 gttgaccttg gccgtctcgg cccgacgcaa cgggaagtgt tcgccgggga cggagaggca
    12841 gccctcggac tcttcgtcgg ggtcaggacg gccgctgggc accttggaga cggcgagaat
    12901 ggggttgacg accactcccc gggcggggac gccgtcatgg ttggccatgt cgtagacgaa
    12961 cacccgcagg cccacgccga tctgcggagc ggcgaggccg acgccgtgtg cggcgtccat
    13021 cgtgtcgtac atgtcctgga ccaactcggc caactcatca tcgaaacgct cgacacggct
    13081 ggcgacgcgg tgcaggacgg gctcaccgtg gatgacgacg ggacggatgg ccatg
//
LOCUS       DAORPW010000060        12882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1005_length_12882_cov_673.825368, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000060 DAORPW010000000
VERSION     DAORPW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1005_length_12882_cov_673.825368"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1157
                     /gene="nagA"
                     /locus_tag="PLB86_06655"
     CDS             39..1157
                     /gene="nagA"
                     /locus_tag="PLB86_06655"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRC64852.1"
                     /translation="MDTLLAADRIFTPEEILEPGWVEVAGTTVKAIGSGHHPHREPDW
                     RTTTLAPGLVDTHVHGGGGASFSTTSGDEAALAAMAHQRAGSTSVIASLVSAPIPVLA
                     DQLACLADLCDDGILAGLHLEGPWISPTHRGAHDLRHLARGNAAAVDRLVEAARGHLR
                     MATVAPEILGGFEVIKALVDADVLVAVGHTAADTATTEEAVEAGARIATHLFNGMPPL
                     HHRFPGPVGVLLANPSVTVELIADGVHLAPATLALAARAAAGRVSLVSDAMSGCGQPD
                     GRYVLGSTPVTVVDGVARVTSSGALAGGTRTLAHGIRTLYAAGVPLADAITAATATPA
                     RALGLWRRGRLVVGANADLVALDDDLAVTRVMVNGTWR"
     gene            complement(1188..3203)
                     /locus_tag="PLB86_06660"
     CDS             complement(1188..3203)
                     /locus_tag="PLB86_06660"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HRC64853.1"
                     /translation="MTAYPHLLAPLDLGFVTLPNRALMGSMHVGLEEVPGGFERMGAF
                     YAERARGGAALIVTGGFGPNEAGALLPGAALLTTPEEADRHRVVTDAVHAAGGRICLQ
                     ILHAGRYAAHLGAVAPSPIQAPISPITPRELTTAEVGETVADFARCAALAQAAGYDGV
                     EIMGSEGYLINQFLVTRTNQRTDEWGGSYAARMRFPVEVVRAVRETVGPNFIVVYRLS
                     MIDLVEDGSTLDEVVELARGVEAAGATMLNTGIGWHEARIPTIATSVPRAGFAWVSGK
                     VKAAVGIPVVATNRINTPDVAEQVLADGLADMVSMARPFLADPLFVTKAAAGQADRIN
                     TCIACNQACLDHTFAGKLTSCLVNPQACHETELIIAPTDAPKRIAVVGGGMAGLACAT
                     TAARRGHAVTMFEADSDIGGQFNLARQIPGKEEFSETLRYFRGELVATGVDVRLGTRA
                     GEGELAAYDTVILATGVTPRIPDVEGVDHPTVVSYLQVLRDHVPVGDTVVIMGAGGIG
                     FDVAEYLTQSGPSGALDPTAFNAEWGIDTTYAHRGGVVAAQPEPPARKVFLVQRKDSK
                     VGAGLGRTTGWIHRAQLAARGVAFVAGARYDRIDDAGLHITIGERAAVLPADTIVLAT
                     GQEPNRELYAALEASGVDVRLIGGADVASELDAKRAIKQGTEVAAAL"
     gene            complement(3278..3982)
                     /locus_tag="PLB86_06665"
     CDS             complement(3278..3982)
                     /locus_tag="PLB86_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC64854.1"
                     /translation="MPPSPRTRPPLHRVTVLDAAVELADAEGIEAVSMRRLAERLGVV
                     PMALYKHVSDKEDLLDGMIDRVIAQFQPGDPSADPSADPSAWPDAVRRVLLSTREVVQ
                     RHPWARRVIETRTHRTPAVLAYMEAVTQLFLGGGLSPDLTHHVMHVLGNRIWGFSPEL
                     FNDPGGRPASDRPRTSGQPVPSDFPGILAIAADASARRPGATGCDEEFEFRFALDLLL
                     DGIARLHASEWASPPL"
     gene            4084..4815
                     /locus_tag="PLB86_06670"
     CDS             4084..4815
                     /locus_tag="PLB86_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC64855.1"
                     /translation="MARVLVVDDEPQIRAILRAYLSAEGFQVQEAATGAQALHLLATE
                     PPDLVLLDIGLPDLDGLEVLRTLRKTSDTYVVLVTARAEEVDKLIGLGVGADDYVTKP
                     FSPREVVARVKAVLRRTRPIGPAATDPGDTDVLHFDSVSVDTGRREVRVGGIPAVLTA
                     LEFDLLVALAESPGRVFSRAQLLEKVWGYDFFGDERVVDVHIRGMRKALGDDATAPRL
                     IGTVRSVGYKFLLDPIDRLTTEGAS"
     gene            4812..6029
                     /locus_tag="PLB86_06675"
     CDS             4812..6029
                     /locus_tag="PLB86_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC64856.1"
                     /translation="MNRLVVRLVISHLLVAVVAALATFLVVRQLAPALFDASLRVGQG
                     GGLGQGSGAQGTAGGAGMVLRQQFGDAVDRALAVGAVVGALAAALVGALAAYRLIRPL
                     DALRAATRAMAQGTYAVPLPTPRTTELAELTDDVKLLGQQLGETETQRVRLLGEVAHE
                     MRTPLTVIDGTVEAMIDGILPLEPDQLAVVSDEVRRLRRLSDDLSALSRTDEGRLSLV
                     LADVDLREVVSRAAGRLRAQAEDAGLDFEIDCGDANVPVRVDAERIGQVVTNLVGNAV
                     RATPEGGRVRVRCGPSESAGRHGAASAVIIVEDTGEGLAGADLTRIFERFYRVPGRRA
                     PEHETGSGIGLTIARGIVAAHGGQLTATSAGPGQGATFTVTLPLARPGESHDRSDVTG
                     LASAEVHNPGGRR"
     gene            6171..6878
                     /locus_tag="PLB86_06680"
     CDS             6171..6878
                     /locus_tag="PLB86_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRC64857.1"
                     /translation="MPIPQGGLPSTRGLLRDEVYARLCDAIVDGTFRPGEQLRDNEIA
                     HWLGVSRTPVREALLRLARIGLVISTPGKSTLVADIEPQTVRDAQEVVTALHELAVRT
                     AIPFIRPGDIETMRRANRRFEAALAAGDADAAIRADDDLHGVAVAVTANLAICSVLEQ
                     YVPVLRRAERLRFSSLSGRRSVTWHERLISAMEVGDADAAAAASTAAWSTLARLIDLE
                     RAIDHANGATDADRPTR"
     gene            6888..8246
                     /locus_tag="PLB86_06685"
     CDS             6888..8246
                     /locus_tag="PLB86_06685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRC64858.1"
                     /translation="MRSRRRRIVLRVLGGLLALVLVALVGGYLYAKPLLLTGTGYAAH
                     NDCAVRAIAGRSDPRADLPPNPLVPYLRSSQAADNSSYSATVLGVLAGQTAYYGDAGC
                     TLGTPLTGTGPLTQVPVGANPFDAVAVPAPSSAVAAAVAKAFGDDLTPFEQDLLGTRA
                     VVVVQGGKLVAERYAPGFTAATPQLGWSMAKSAASLLTGRVVKEGKVAVTDAGLRPEW
                     TDDRSKITVDQLMRMTSGLTWNEDYDLGTAITAMLYLEPDMAKFVASQPLAHAPGTYQ
                     QYSSGSTNLLCSVLRQRTGLGVDLPRRELFAPLGLSSAVWEPDGSGLPVCSSYLWATP
                     RDWAALGQFALQDGVWGGQRLLPEGWMAATTRSDAVATTEDPGYAAGWWANRLADGTL
                     DQPTLPADTYRANGHDGQRIYVVPSADLVVVRLGFSPSIKTPEQLRVVDLVATLAGMP
                     RA"
     gene            8396..8821
                     /locus_tag="PLB86_06690"
     CDS             8396..8821
                     /locus_tag="PLB86_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRC64859.1"
                     /translation="MTQPVRDRKTQPTQGDVDAHLASITHEGRRADAQAAVELFREVT
                     GAEPQMWGTSMIGFGRMIYTGSDHKMRETMAVGLAARAAALTLYGLTFYGSNEALLAR
                     LGPHTTGKGCLYLKRLSDVDVDVLRELIARGWTENHQPY"
     gene            complement(8853..9146)
                     /locus_tag="PLB86_06695"
     CDS             complement(8853..9146)
                     /locus_tag="PLB86_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRC64860.1"
                     /translation="MLADEPGLREQRMFGGLGFMLDGHMAVAASSDGGILLRIDPAAA
                     GDLVDGVVARRFEMRGRAMDGWLAVDPAAAQTDEALREWVAHGVAYVRSLPPK"
     gene            complement(9189..9761)
                     /locus_tag="PLB86_06700"
     CDS             complement(9189..9761)
                     /locus_tag="PLB86_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRC64861.1"
                     /translation="MAEILLFHHIQGLTHGVIAFADALRAGGHTVHTPDLFDGRTFDS
                     IEAGQAYLGEVGFDEVRERGILAAEGLPAGLVYAGFSLGVMPAQQLTQNRPGARGALL
                     YHSFADPSYFGTWPVGVPVQIHSMDADPFFVGEGDIEPAEAFVAAHDDAELFLYPGGG
                     HLFADSSLADYDAVATEIVIERSLALLARV"
     gene            complement(9754..10353)
                     /locus_tag="PLB86_06705"
     CDS             complement(9754..10353)
                     /locus_tag="PLB86_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC64862.1"
                     /translation="MTTQPGRARPTDADGFRAAWALAEAEWAQTFARARSFSAQQLHE
                     RVDGEWSFIQTQRHLVFATDAWVRRALLGDPTPWHPLDLPHDDMDDAPGVPRDDAARP
                     TLDEVLALRADRMSTVREVIAGLTDEALDSLTVPVLEPGYPESEAFLVRRCLGAVVNE
                     EWEHRRFAERDLDIIDNGSRSTDQTTETDQPREEETTHG"
     gene            complement(10364..11047)
                     /locus_tag="PLB86_06710"
     CDS             complement(10364..11047)
                     /locus_tag="PLB86_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC64863.1"
                     /translation="MTDLAATVEGIGALAEPVRRALYDFVVASRGPVGREEAAEAVGV
                     PVHTAKFHLDRLADDGLLDVEFQRRTGRTGPGAGRPAKLYRRSTQEFAVSLPPRHYDL
                     AGRILADAVEQAAAGVPLDGALRAAAERAAYRVVESVPTDGDRLPQVLTGLGYEPREE
                     EAGLRLVNCPFHSMAAEHTALVCGVNRDFVQGVLDALGRGDVQARLTPTPGQCCVTVV
                     PTTDVGARA"
     gene            11139..11801
                     /gene="folE"
                     /locus_tag="PLB86_06715"
     CDS             11139..11801
                     /gene="folE"
                     /locus_tag="PLB86_06715"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HRC64864.1"
                     /translation="MSQAATPTVPAGEAPTAYPLLGPRRPRVTGHAPLVDLGRAEAAA
                     TDLLRALGLPIDTEAMCDTPGRLTRAYLELLSVPDFEMTTFANDEGYDELVVVADIPV
                     RSLCEHHMLPFVGVAHVGYVPGDRLLGLSKFARTVDFFARRPQTQERLTSQIADHLDA
                     HLSPQGVGVVISAEHTCMTLRGARAAGTRTRTSATRGVLRNHPATRAEFLSRAATLEE
                     SR"
     gene            11798..>12882
                     /locus_tag="PLB86_06720"
     CDS             11798..>12882
                     /locus_tag="PLB86_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC64865.1"
                     /translation="MNPIVVVGAGLAGATAVTALREGGHDGPLVLLGAESHPPYERPP
                     LSKGYLLGNDPFESAFVHEPQWYAAHDIDLRTGAFVTDLDLGRKSVSLLDGTEIAYDQ
                     LLLATGSRPRRLPLADDSGVPTAYLRTVEDSDRIRAAFGPGHRIVIIGAGWIGLEVAA
                     AARAAGTEVTVFEVAELPLLRVLGPRVAQAFADLHRAHGVDLRLGTAVSAADLAGATL
                     VVVGVGVAPDASLAQRAGLAVDNGVLVDATLRASHPGVYAVGDIANHDHPLLGRRVRV
                     EHWDTAIEQAKVAAHNLVGGQEAEAYDRLPYFFTDQYDLGMEYVGYAAADADTEVVVQ
                     GDLGAEQWRAYWVSGGVVVAGMHVNEWG"
BASE COUNT         1970 a         4573 c         4436 g         1903 t
ORIGIN      
        1 cgagtgagga acgacgaacg gaaggggtgc gaggcgtcat ggacacattg ctggcggccg
       61 accgcatctt cactcccgag gagattctcg agccgggctg ggtcgaggtg gctggcacaa
      121 ccgtcaaagc catcggatca ggccaccacc cgcaccgtga acccgattgg cgcacaacga
      181 ctctcgcccc cggcctggtg gacacgcacg tccacggcgg cggcggggcc tcgttctcga
      241 cgacgtccgg cgacgaggct gccttggccg cgatggccca tcagcgagca ggcagcacga
      301 gcgtcatcgc cagcctcgtc tccgctccca ttccggtgct ggccgaccaa ctggcctgtc
      361 tcgccgacct gtgcgacgac ggcatcctcg ccggtctgca cctcgaaggc ccctggatca
      421 gccccactca tcgcggcgcc cacgacctgc gccacctcgc tcgcggcaac gcggcggccg
      481 tcgaccggct cgtcgaggcg gcccgcgggc atctgcggat ggcgacggtt gcccccgaga
      541 tcctcggtgg cttcgaggtc atcaaggcgc ttgtcgacgc ggatgtcctt gtcgccgtag
      601 gccatacggc cgccgacacg gcaactaccg aggaggctgt cgaggcgggt gcccggatcg
      661 ccacccacct gttcaacggt atgccgccgc tgcaccatcg cttccccggc ccggtgggcg
      721 tcctcctcgc caacccatcg gtcaccgtcg agctcatcgc cgacggagtg cacctggcgc
      781 ccgcgaccct ggccctcgcc gcccgcgcag cagccggacg ggtgagcctg gtcagcgatg
      841 cgatgtccgg ctgcggccag cccgacgggc gctacgtcct cggctcgacg ccggtgacgg
      901 tcgtcgacgg ggtagcccgc gtgacgtcct ccggtgcctt ggcaggcggc acgcggactc
      961 tcgcgcacgg catacgcacc ctgtatgccg cgggcgttcc cctcgcggac gccatcaccg
     1021 ctgccacggc gacccctgcg cgagcgctcg ggctgtggcg ccgtggccga ctcgtcgtcg
     1081 gcgccaacgc cgatctcgtg gccctcgatg acgacctcgc tgtcactcgg gtcatggtca
     1141 acgggacctg gcgctgaccg ctcgacgacg agccccggca gcatcggcta caaagccgca
     1201 gcaacctccg tgccctgttt gatcgcccgc ttggcatcga gttcactggc gacgtcggcc
     1261 ccaccgatga gccgcacgtc gacaccggaa gcctcgaggg cggcatacaa ctcgcggttg
     1321 ggctcctgcc cggtggccag gacgatggtg tcggccggaa ggacggcggc gcgttcaccg
     1381 atggtgatgt gcaggcccgc atcgtcgatc cggtcatacc gagcgccggc gacgaaggcg
     1441 accccgcggg cggccagctg tgcgcggtgg atccagccgg tcgtgcgccc gaggcccgca
     1501 ccgaccttgg agtccttgcg ctgcacgagg aacaccttgc gcgccggcgg ttccggttgc
     1561 gccgcaacga cgccgccgcg atgggcgtag gtggtgtcga tcccccactc ggcgttgaac
     1621 gccgtcgggt ccagggcgcc gctcgggccc gactgagtga gatattcggc gacgtcgaag
     1681 ccgatgccgc ccgcgcccat gatgacgacc gtgtcaccga cggggacgtg gtcgcgcagc
     1741 acctgcaggt aggacacgac cgtggggtgg tcgacgccct cgacgtcggg gatgcgcgga
     1801 gtcacgcccg tggccaggat gacggtgtcg tatgccgcca gctcaccctc cccggcacgg
     1861 gtcccgagcc gcacgtcgac cccggtggcc accaactcgc cccggaagta gcgcaaggtc
     1921 tcgctgaact cctccttgcc ggggatctgg cgcgccaggt tgaactgccc gccgatatcg
     1981 gagtcggcct cgaacatcgt caccgcgtgg ccacgacgcg ccgccgttgt cgcacaagcc
     2041 aatccggcca tgccaccccc gacgacggca atccgcttcg gtgcatcggt gggggcgatg
     2101 atcaactcgg tctcatggca ggcctgcggg ttgaccaggc aagacgtgag cttgccggcg
     2161 aacgtgtggt ccaggcaggc ctggttgcag gcgatgcagg tgttgatccg atctgcctgt
     2221 cccgcagccg ctttggtgac gaacagggga tcggcgagga acgggcgggc catcgacacc
     2281 atgtcggcga gcccgtcggc caacacctgc tcggccacgt caggagtgtt gatccggttg
     2341 gtcgcaacga ccggaatgcc tactgccgct ttgactttgc cgctcaccca cgcgaaccca
     2401 gcgcgcggaa cgctggtggc aatcgtcggg attcgggcct cgtgccagcc gattcccgtg
     2461 ttgagcatgg tcgcgcccgc cgcctcgacg ccccgcgcga gctcgacgac ctcatcgagc
     2521 gtcgagccgt cctcgaccag gtcgatcatc gaaagccgat agacgacaat gaaattcggg
     2581 ccaaccgtct cgcgaaccgc ccggacgact tcgaccggga agcgcatccg cgcggcatac
     2641 gacccgcccc actcgtccgt gcgctggttg gtgcgggtga cgaggaactg gttgatgagg
     2701 tagccctccg accccatgat ctcgaccccg tcgtagcccg ccgcctgcgc gagcgcagcg
     2761 cagcgcgcga agtcggccac ggtctccccc acctcggcgg tcgtcaactc gcgcggggtg
     2821 atcggcgaga tcggggcctg gatggggctc ggcgcgacgg cgccgaggtg cgcggcatac
     2881 cgaccggcat gcaggatttg caggcagatt cggccgcccg cggcgtgaac cgcgtcggtg
     2941 acgacccggt ggcgatccgc ttcctcgggg gtcgtcagta gggccgcacc cggcagcagg
     3001 gcgcccgcct cgttgggccc aaagccgccg gtgacgatga gcgcggcgcc gccgcgagcg
     3061 cgctcggcat agaaggcccc catccgctcg aagccgccgg gcacctcctc cagccccacg
     3121 tgcatcgagc ccatgagcgc tcggttggga agggtgacga agccgagatc gagcggggcc
     3181 agcaagtggg ggtatgccgt cacggcggtc tccgtcgctt cgcctgggac gtccctcggt
     3241 cggcacgccc gcgtgtggcc tcgatgttac tcgtcagtca caacggcggc gaggcccact
     3301 cggaggcatg cagtcgcgcg atcccgtcca ggagcaggtc gagcgcgaag cggaactcga
     3361 actcctcatc gcagccggtc gcgccggggc gccgcgccga ggcatccgcc gcgatcgcga
     3421 ggatgccggg gaagtcgctc ggaacgggct ggccgctcgt tcgcggccga tccgacgccg
     3481 gacggccgcc ggggtcgttg aacaactccg ggctgaagcc ccagatccgg ttgccgagca
     3541 cgtgcatcac atggtgggtg aggtcaggcg agagcccgcc gccgaggaac aactgggtga
     3601 cagcctccat ataggcgagc accgcggggg tgcggtgggt gcgggtctcg atgacccgcc
     3661 gggcccaggg gtggcgttgg acgacctcgc gcgtcgacag cagcacccgg cgaaccgcat
     3721 cgggccacgc gctggggtcc gcgctcgggt ccgcgctggg atcgccaggc tggaactggg
     3781 cgatgacccg gtcgatcatc ccgtcgagga gatcctcctt gtcggacacg tgcttataga
     3841 gagccatcgg gacgacgccc agccgttcgg caagcctgcg catgctcacc gcctcgatgc
     3901 cctcggcatc ggccaactcg acggccgcat caagcaccgt cacccggtgc agcggggggc
     3961 gggtccgggg ggagggcggc atacgggctc ctgggcaggg ggctgacggg tgtacatcgt
     4021 acgctttcgc gcagatgcgg ccggtgtctc tagcctggga gcagacagaa ggaggtgcaa
     4081 cggatggcca gggtcctggt cgtggacgac gaacctcaga tccgcgccat cttgcgggcc
     4141 tacctgagcg ccgaagggtt ccaggtccag gaggcggcga ccggcgccca ggcgttgcac
     4201 ctactcgcca ccgagcctcc cgacctcgtc ctgctcgaca tcgggctgcc cgacctcgac
     4261 ggcctcgagg tcctgcggac cctgcgcaag acctccgaca cctacgtcgt cctcgtcacc
     4321 gcccgggcgg aagaggtcga caagttgatc gggttggggg tcggcgccga tgactacgtc
     4381 accaaaccct tcagcccgcg cgaggtcgtc gcgcgcgtca aagctgtgct ccggcgtacc
     4441 cggccaatcg gcccggcggc gaccgatccc ggcgacaccg acgtgctcca cttcgacagc
     4501 gtcagcgtcg acaccggtcg cagggaggtg cgggtgggcg gcataccggc cgtcctcacg
     4561 gccttggagt tcgacctgct ggtggcgctc gccgaatctc cggggagggt cttctcgcgg
     4621 gcccaactgc tggaaaaggt ctggggctac gacttcttcg gcgacgaacg ggtcgtcgac
     4681 gtccacatcc gcgggatgcg caaggccctg ggtgacgacg cgaccgcgcc gcgcctcatc
     4741 gggaccgtcc gcagcgtcgg ctacaagttc ctcctcgacc cgatcgaccg cctcacaacg
     4801 gagggcgcgt catgaaccgg ctcgtcgtgc gactggtgat ctcgcacctg ctcgtcgccg
     4861 tggttgcggc cctggccact ttcctcgtcg tgcgccaact cgcgccggcc ctcttcgatg
     4921 ccagcctgcg ggtcggccag ggcggtgggc tggggcaagg atccggcgcc caggggaccg
     4981 ccggaggcgc cggaatggtg ctgcgccaac agttcggcga cgccgtcgac cgggccttgg
     5041 ccgtcggagc cgtggtgggc gcactggcgg ccgccctcgt gggggcgctc gcggcatacc
     5101 gactcatccg gccgctcgac gccctccgcg cggcgacgcg ggccatggcg caggggacgt
     5161 atgccgtccc gctccccaca ccgcgcacga ccgagctcgc cgaactcacc gacgacgtca
     5221 agctcctggg gcagcaactc ggcgagaccg agacccagcg ggtccggctg ctcggtgagg
     5281 tggcccatga gatgcggacc ccgctgaccg tcatcgacgg gacggtggag gcgatgatcg
     5341 acggcatcct gcctctggaa cccgaccagt tggccgtggt gagcgatgag gtgcggcggc
     5401 tacggcggct gtccgacgac ctgtcggcgc tgtcgcgcac cgacgagggc cggctgtccc
     5461 tcgtcctggc cgacgtcgac ctgcgggagg tcgtgagtcg ggcggccgga cggctgcggg
     5521 cgcaggccga ggacgcgggg ctggacttcg agatcgattg cggcgacgcg aatgtccccg
     5581 tgcgagtgga cgccgagcgg atcggccagg tcgtcacgaa cctggtcggc aatgccgtcc
     5641 gtgccacccc ggaaggtggc cgggtgcggg tgcggtgcgg gccgtccgag agcgccggac
     5701 gacacggcgc ggcgtcggcc gtgatcatcg tcgaggacac cggcgagggg ctggcaggcg
     5761 ctgacctcac gcggattttc gagcgcttct accgggtgcc ggggcgccga gcccccgaac
     5821 acgaaaccgg atccggaatc ggcctgacca tcgccagagg catcgtggct gcgcacggag
     5881 ggcagctcac agccacgtcc gccggccccg ggcagggggc gaccttcacg gtgacgctgc
     5941 cactcgcgcg gcccggggag agtcatgacc gctccgacgt caccggcctt gcgtcagccg
     6001 aggtccacaa cccgggtggc aggcgctgac tcggccaaac ccccggtgac tcctcccagt
     6061 catagcccac gatctgtgac ttcggacacg aaaggacaac ataagtcttc agacgcccgg
     6121 aaagtccgaa gcctcctacg ggaggcaggg ttgcgtgtat caatagaccc atgcccattc
     6181 cccagggcgg gttgccgtcc acccgcggac tgctgcgcga cgaggtctac gcgcggctgt
     6241 gcgatgcgat tgtggacggg acgttccggc cgggcgaaca gttgcgcgac aacgagattg
     6301 cccattggct cggagtgagc cggactccgg tgcgggaagc cctgctgcgg ctggctcgca
     6361 tcggcctggt gatttcgacg cctggcaagt cgacgctcgt tgccgacatc gagccgcaga
     6421 cggtccgcga cgcccaagag gtcgtgaccg cgctgcacga gttggcggtc cgcacggcga
     6481 tccccttcat ccggcccgga gatatcgaaa ctatgcgtcg ggctaaccgc cgcttcgaag
     6541 cggcgttggc agccggtgac gccgacgcgg cgatccgcgc cgacgacgac ctgcacggcg
     6601 tcgcggtcgc cgtcacggcc aacctcgcga tctgttccgt gctcgagcag tatgtgcccg
     6661 ttctgcgacg ggccgagcga ctgcgcttct catcgctctc gggtcgacga tcagtgacct
     6721 ggcacgaacg gctcatcagc gccatggagg tcggcgacgc cgacgccgcc gccgcggcgt
     6781 cgaccgcggc gtggtcgacg ctggcgcgcc tcatcgatct cgagcgggcg atcgaccacg
     6841 cgaacggcgc gaccgacgcc gaccgaccca cgcggtagcg tccgctcatg cgttcacgtc
     6901 gccgccggat cgtgcttcgg gtgctgggtg ggctgctcgc cctcgtcctc gtcgcgctcg
     6961 tggggggcta cctctacgcc aagccgctac tgctcaccgg cacggggtat gccgcccaca
     7021 acgactgcgc cgtgcgcgcc atcgcgggtc gttccgaccc gagggccgac ctgccgccga
     7081 acccgctcgt gccgtatctg cgcagttcgc aggctgcgga caacagctcc tactccgcca
     7141 ccgtcctcgg ggtgttggcc ggccagacgg cctactacgg cgatgcgggc tgcacgctgg
     7201 ggaccccgct cacgggaacc gggccgctga cccaggtgcc cgtcggggcg aacccgttcg
     7261 acgccgttgc ggttcccgcg ccgtcctcgg ccgtcgctgc agccgttgcg aaagccttcg
     7321 gcgacgacct cacgccgttt gaacaggacc ttctggggac gcgagccgtc gtcgtcgtgc
     7381 agggtggcaa gctcgtggcc gagcgctatg cccccggatt caccgctgcc acaccgcagt
     7441 tggggtggtc gatggccaag agcgccgcga gcctgctcac cgggcgggtc gtcaaagagg
     7501 gcaaggtcgc cgtcaccgac gccgggctgc gtccggagtg gaccgatgac cgctccaaga
     7561 tcaccgtcga ccaactcatg cggatgacga gcgggctgac gtggaacgag gactacgacc
     7621 tcggcacggc gatcaccgcg atgctctatc tcgagcccga catggcgaaa ttcgttgcga
     7681 gccaaccact tgcccatgcc cccggcacct accagcagta ctccagcggc tcgaccaacc
     7741 tgctctgctc ggtgctgcgc caacgcaccg gattgggcgt cgatctgccg cgccgcgagc
     7801 tgtttgcccc cttgggtctc tcgtccgccg tgtgggagcc ggacggctcc gggctgccgg
     7861 tgtgcagttc ctacctctgg gcgacgccgc gcgactgggc ggcgctcggc cagttcgcgt
     7921 tgcaggacgg cgtgtggggc ggacagcgac tgctcccgga gggctggatg gccgcgacga
     7981 cccgctccga cgccgtcgcc acgaccgagg accccggata tgccgccggc tggtgggcca
     8041 accgccttgc ggacggcacg ctcgatcagc cgaccttgcc cgccgacacc tatcgggcga
     8101 acggccatga cggacaacgg atctacgtcg tcccgtcggc cgacctcgtg gtcgtccggc
     8161 tcgggttcag cccctcgatc aagacaccgg agcagctgcg ggtcgtcgac ttggtcgcca
     8221 ctctcgccgg tatgccgcgc gcctgaccca acgccccgcc acggcgagtc agggcgagtt
     8281 catcgggcag gcgttgttcc agtgacgaca cgttcgacaa gacgagcgac gacacaggtg
     8341 gcgacacgag tgacgtcgcg cagggcgagg cgcggggagg cagcacacta ggccgatgac
     8401 ccagcccgtg cgcgaccgta agacccagcc gacccagggg gacgtcgacg cccatctcgc
     8461 gtcgatcacc catgaggggc ggcgagcgga cgcccaggcc gcggtcgagt tgttccgcga
     8521 ggtcaccggc gccgagccgc agatgtgggg aacctcgatg atcggtttcg gccggatgat
     8581 ctacaccggt tcggaccaca agatgcgcga gacgatggcg gtcgggctgg ccgcgcgggc
     8641 ggctgcgctc acgctctatg gcctgacctt ctacggttcg aacgaggcgc tgttggcccg
     8701 gctcggacct cacaccaccg gcaagggatg cctctatctc aagcggttgt ccgatgtcga
     8761 cgtcgacgtc ctgagagaac tcatcgcccg cggctggacg gagaaccacc agccctactg
     8821 acgtcgccga gccgcggcgg gccgggcaag cgctacttcg gcggcaacga gcggacgtag
     8881 gcgaccccgt gagcgaccca ctctcgcaac gcctcatcgg tctgggcggc tgccgggtcg
     8941 acggccagcc agccgtccat cgcccgccca cgcatctcga accgccgcgc cacaactccg
     9001 tcgacgaggt caccggccgc cgcgggatcg atccgcagga gtatgccgcc atcgctactg
     9061 gccgcgacgg ccatgtgccc gtcaagcatg aagcccagcc cgccgaacat ccgttgctcg
     9121 cgcagcccag gctcgtctgc cagcacctca cgcagccggt cggccaactc aggggtcgag
     9181 ggccaccgtc agaccctggc caggagtgcc aacgagcgct caatgacgat ctctgtggcc
     9241 acggcgtcgt aatcggccag cgaactgtcc gcaaagagat gccccccacc gggatagagg
     9301 aacaattcgg cgtcgtcgtg ggccgccacg aaggcctcgg ccggctcgat gtcgccctca
     9361 ccgacgaaga aggggtcggc atccatcgaa tggatctgca cggggacacc gacgggccag
     9421 gtgccgaagt aggacgggtc ggcgaacgag tgatagagca gcgcgccgcg ggcacccggc
     9481 cggttctgag tcaactgctg ggccggcatc acgccgaggg agaacccggc atacaccagg
     9541 cccgcaggga gcccctccgc ggcgaggatg ccgcgctccc gcacctcgtc gaagcccacc
     9601 tcgccgagat aggcctgccc ggcctcgatc gagtcgaagg tccgcccgtc gaacaggtcc
     9661 ggcgtgtgca ccgtgtgccc acccgcccgc agtgcgtcgg cgaacgcgat caccccgtgc
     9721 gtcagccctt ggatgtggtg gaacaacagg atctcagcca tgcgtggtct cctcttcccg
     9781 aggctggtcg gtttccgtgg tctggtcggt gctccgcgaa ccgttgtcga tgatgtccag
     9841 gtcgcgctcg gcgaaccgac gatgctccca ctcctcgttg acgaccgccc ccaggcatcg
     9901 acgcaccagg aacgcttcgg actcgggata tcccggctca agcacgggca ccgtcagcga
     9961 gtcgagggcc tcgtcggtca gcccggcgat cacctcacgc accgtgctca tccggtcggc
    10021 ccgcagggcc agcacctcgt ccagcgtcgg gcgagcggca tcatcccgcg ggacgcccgg
    10081 ggcatcatcc atgtcgtcgt gcggcagatc caacgggtgc cacggcgtcg ggtcaccgag
    10141 cagcgcccgg cgcacccagg catcggtcgc gaagacgagg tgccgttggg tctggatgaa
    10201 cgaccactcg ccgtcgaccc gctcgtgcaa ctgttgcgca ctaaaactcc gcgcccgcgc
    10261 gaaggtctgg gcccactccg cctcggccaa cgcccacgcg gcgcggaagc cgtcggcgtc
    10321 ggtgggccgc gcccgaccag gctgtgtcgt catggccgac accttaggcg cgagcaccga
    10381 catcggtcgt cggaaccacc gtcacgcagc attgccccgg cgtcggggtg agccgcgcct
    10441 ggacgtcacc cctgcccaac gcgtcgagca ctccctgaac gaaatcgcga ttgaccccgc
    10501 acaccagcgc cgtgtgctcc gccgccatcg agtggaacgg gcagttgacc aggcgcagcc
    10561 ccgcctcctc ctcccgaggc tcgtagccca acccggtgag cacctggggc aaccgatccc
    10621 cgtccgtggg cacggactcg accacccggt atgccgcccg ctcggcggcc gctcgcagcg
    10681 caccgtccag gggcacaccg gcagcggctt gctcgaccgc atcggcgagg atgcggcccg
    10741 cgaggtcgta atgccggggc ggcagggaga ccgcgaactc ctgggtcgag cgtcgataga
    10801 gcttggctgg acggccggct cccgggccgg tgcgaccggt gcgccgctgg aactccacgt
    10861 cgagcagacc gtcgtcggcc aaccggtcga ggtggaactt cgcagtgtgc acggggacgc
    10921 cgaccgcctc agcagcctcc tcgcgcccca ccggcccgcg gctggcgaca acgaagtcgt
    10981 agagggcgcg ccgcaccggc tcggcgagcg cgccgatgcc ctcaacggtc gccgcgaggt
    11041 cggtcatgag ccatcctgat ttctaaaaga ttctttgatt gactttagaa cgggaggaac
    11101 gtaacgtcaa ccctagtctc cgttagaaag gggttcagat gtcccaggcc gccactccca
    11161 cggtccccgc aggcgaagcg cccacggcat accccttgct cggcccgcgc cgcccccggg
    11221 tgacggggca cgccccactc gtggaccttg ggcgagccga ggccgcggcc accgacctgc
    11281 tgcgggccct cgggctgccg atcgacaccg aagcgatgtg cgacacccct gggcggctga
    11341 cccgggccta tctcgagctg ctcagcgtgc ccgacttcga gatgacgacc ttcgccaacg
    11401 acgagggata tgacgagctc gtcgtcgtgg ccgacatccc cgtgcggtcg ttgtgtgagc
    11461 accacatgct gccgttcgtc ggggtcgcgc acgtgggtta tgtgcccggt gaccggctgc
    11521 tggggttgtc gaagtttgcc cggaccgtcg acttcttcgc ccgccgaccc cagactcagg
    11581 agcgactcac cagtcagatc gccgaccacc tggacgcgca cctgagcccg cagggggtcg
    11641 gcgtcgtcat cagcgccgag cacacctgca tgaccttgcg gggcgcccgc gccgcaggca
    11701 cccgcacccg cacctccgcc acccgcggag tcctgcgcaa ccacccggcc acccgcgccg
    11761 agttcctttc gcgcgcagcc acattggagg agtcccgatg aaccccatcg tcgtcgtcgg
    11821 cgcaggcttg gcgggcgcca ccgcagtgac cgcgttgcgc gaggggggtc acgacggtcc
    11881 gctcgtcctg ctcggcgccg agagccatcc gccctatgag cgcccgccgc tgtccaaggg
    11941 ttacctgctc ggcaacgacc cgttcgagag tgccttcgtt cacgaaccgc agtggtatgc
    12001 cgcgcacgac atcgacctgc gcaccggcgc cttcgtcact gatctggacc tcgggcggaa
    12061 gtccgtcagc ctgctcgatg gtaccgagat cgcctacgac cagctgctgt tggcgaccgg
    12121 gagccggcca cggcggttgc cgctggcgga cgactcgggg gtgcccacgg catacctgcg
    12181 gacggtcgag gacagcgacc ggatccgtgc ggcgttcggg ccggggcacc ggatcgtcat
    12241 catcggggcg ggctggatcg gactggaggt ggcggccgcg gcgcgcgctg cagggaccga
    12301 ggtgaccgtc ttcgaggtgg cggagttgcc gctgctgcgg gtgctcgggc cgcgggtcgc
    12361 gcaggcgttc gccgacctgc accgcgctca cggggtggac ctgcggttgg ggactgcggt
    12421 cagtgccgcg gacctggcgg gcgccactct cgtggtcgtc ggcgtggggg tcgctcccga
    12481 tgcgtcgctc gcccaacgcg cggggctcgc tgtcgacaac ggggtgctcg tcgacgcgac
    12541 cctgcgcgcc agccaccctg gcgtgtatgc cgtgggcgac atcgccaacc acgaccatcc
    12601 gttgttgggg cggcgggtgc gggttgagca ctgggacacc gcgatcgagc aggccaaggt
    12661 cgcggcgcac aacctggtcg gggggcagga ggcagaggct tacgaccggc tcccctactt
    12721 cttcaccgac cagtacgacc tcggcatgga atacgtcggg tatgccgccg ctgacgccga
    12781 caccgaggtc gtcgtgcagg gcgatctggg ggccgagcag tggcgggcct attgggtgtc
    12841 cggcggcgtg gtcgtcgccg gcatgcatgt caacgagtgg gg
//
LOCUS       DAORPW010000061        12631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1039_length_12631_cov_444.345499, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000061 DAORPW010000000
VERSION     DAORPW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12631
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1039_length_12631_cov_444.345499"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLB86_06725"
     CDS             complement(<1..418)
                     /locus_tag="PLB86_06725"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRC64866.1"
                     /translation="MATEPALAVAAPPAVTGDAQIGLASQLGRFAVVGAVSTVVHLGL
                     FALLHLAWPSQVANVAALVVATVVNTALNRRWTFRVTGGRAWRQHAQGFVVFLLTWGA
                     TSGGLALLDLGWPTAGTPIKLLALVAATGISTVVRFV"
     gene            complement(436..2385)
                     /locus_tag="PLB86_06730"
     CDS             complement(436..2385)
                     /locus_tag="PLB86_06730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRC64867.1"
                     /translation="MTELAMAPTSTTGPDQTTATTAATSSRDPSWVRPALLGLLAATA
                     VFYLWNLTSSGYANAFYSAAAQAGADSWKAFFFGSSDAANAITVDKPPASLWFMALSV
                     RIFGLSSFAILLPEVLMGVATVGVVYATVKRQFGAAAGLLAGGILALTPVAVLMFRFN
                     NPDALLVLAMALAAWATVRSIDESSPRWFALVGVFIGLAFLTKTLQAFLVVPGFALAY
                     LVAARPPLLRRIWHTVIGGLAIVASAGWWVAVVELLPASVRPYIGGSQTNSFLELTFG
                     YNGLGRINGEETGSVGNASARLTQIGLGRMFNSENGGQISWLLPAALILLLVGLAYRA
                     RMPRTDARRAAYLVWGSWLVITTLVFSLMAGIYHAYYTVALAPAIAALVAMGGAEAWE
                     RRDRWIGSVPLAAVTVITGVWSFVLLSRVTSWNGWLRVVVLALGLAAAAGFLLADRLP
                     AKAVPAVLGVALAAGLLGPAAYSVATVATPHTGSIVLAGPATSGGAGRAPGGMPPGGT
                     SRGGAGGGLLNASTPSAAVVQALSADASSYTWVAAAVGSQNAAGLQLGTQLPVMAIGG
                     FNGSDPSPTLAQFQEYVAAKQIHYFAAGGGFGRQNGGSRESLSIASWVSANFRAVTIG
                     GSTFYDLTQPASGGVGSTPAATRTA"
     gene            complement(2524..4089)
                     /locus_tag="PLB86_06735"
     CDS             complement(2524..4089)
                     /locus_tag="PLB86_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC64868.1"
                     /translation="MSEQPPAAAAGTAVAAAPAEFVAPAPTTAARSWHPSTWTLRSKL
                     VASMLVLFTVMTLVIAAASVLSLDRSLTAQLDDQLRSSLSKIPPQLGGTASSTSLRDG
                     DGDLGRGPGDEGFVVVVANGTPYTRQRDAITVAQIQLLQEAGLGNQPRNIDLGGELGE
                     YRVLAGTSARNSGAVVYVGLPRDRQVGTVGNAFRTALFASVLGLLAVGIGGAWLVTRN
                     LRPLTRVADTATRVSQLHLSSGQVALAERVPEEDTDPRTEVGRVGLALNELLDHVDSA
                     LNARHESEQRVRQFVADASHELRTPLASIRGYAELTRKEREPVPAGVVHAISRVESEA
                     KRMSTLVDDLLLLARLDAGRPLDQEDVDLTEIVVNAVSDAHAASPQHKWRLDLPDEPV
                     LVRGDSARLHQIVANLLGNARVHTLPGTTVLTSISREPGTVRLSVHDDGPGVPEAQQA
                     KVFQRFARGDSARSRTGGSTGLGLSIVAAVASAHGGSVQLTSTPGDTVFSVVLPAPDD
                     ASSTPRWAGRVTP"
     gene            complement(4089..4826)
                     /locus_tag="PLB86_06740"
     CDS             complement(4089..4826)
                     /locus_tag="PLB86_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC64869.1"
                     /translation="MTTNPVKPQKLERPDGTPPRVLVVDDEHNLTELLSMALRYEGWE
                     VRSAGTGMAAVRAAREFEPDAVALDMMLPDMDGLEVLRRMREHNDRLPVLFLTARDAV
                     EDRIAGLTAGGDDYVTKPFSLEEVVARLRALMRRTVLVATEEDDSRLVVGDLTLDEDS
                     HEVTRAGAQIQLTATEFELLRYLMRNPKRVLSKAQILDRVWNYDFGGQANVVELYISY
                     LRKKIDAGREPMIHTMRGVGYVLKPAS"
     gene            complement(5002..5139)
                     /locus_tag="PLB86_06745"
     CDS             complement(5002..5139)
                     /locus_tag="PLB86_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64870.1"
                     /translation="MTAYVGRRRGANQAARMRDWTTILLGLLCALVVLSIPLGLLAAR
                     G"
     gene            complement(5303..6310)
                     /locus_tag="PLB86_06750"
     CDS             complement(5303..6310)
                     /locus_tag="PLB86_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64871.1"
                     /translation="MTLDLQLLDPDDERQLDAIVALFAATDAVDAPWRHGATPRSIRQ
                     EHLLGYDGDPEPFYLIRRGSDLVAVGAMALPSRDNLDSGWIYAAVHPDARRQGIGRWT
                     YTELIRLATDAGRTVLGTFGVDLPHTRGFVDAIGFEVRSRSVTRRVTFADLPAGATEA
                     AYAQALPYAADYDLLRVVGALPDDLMAGYVQAVAAINDAPTDDLEQEDEVFDAERVRA
                     YERSHAVRGETLYRVIARHRETGEFAGHTVVTVDPERPTFGDQHDTTVVDAHRGHRLG
                     VLLKTDMVRWLGEVEPACGMLTTSNAESNTHMIAVNDLLGYRPISHGIACQRTPSSPA
                     V"
     gene            complement(6389..7444)
                     /locus_tag="PLB86_06755"
     CDS             complement(6389..7444)
                     /locus_tag="PLB86_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HRC64872.1"
                     /translation="MIREVSGNLLTADVDALVNTVNTAGVMGKGIALQFKRAYPAMFT
                     AYEKAAKAGELQPGHMHVWPTNQLDGPRYVINFPTKRHWRSPSRIDDVAEGLADLRRV
                     ISELGITSVAVPPLGCGNGGLAWADVRPMIIGALGDLDGVEVRVYPPAGAPSAAEMID
                     NRPVAALTRGRAALLAMMRGYQEATFEAPSVIEVQKLMYFLQAAGEALQLKFVRHHYG
                     PYADNLRAVLRELEGQYIVGFGDGSQRVNEAEPLSLVSGHDTDIDAALTHQEETLERM
                     SRVLALADGFESAYGLELLASTHWVALHDSRAARDPKIAAAAVQQWSPRKGRLFTTDH
                     VEVAWEALRRNGWLPAA"
     gene            complement(7461..8102)
                     /locus_tag="PLB86_06760"
     CDS             complement(7461..8102)
                     /locus_tag="PLB86_06760"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HRC64873.1"
                     /translation="MPRPEPTWVCHFTHVEHLASIVQHGLVCDSRAHEPGVLQIEVGN
                     TGIKEGRGRRVVPVGPGGVVADYVPFYYAPRSPMMSSIHHGRVPTYTDGCDRLIYVVT
                     STQRLRELGLNVLGTDRHPLKSVAVFADDDGDIAAMTDWPLMSARYWANTPEDLSRRE
                     RRMAECLVHQVVPFHAILGLVAKSGAIRDEAAGVLASMGVTWVQTAVRPDWYF"
     gene            complement(8089..11328)
                     /locus_tag="PLB86_06765"
     CDS             complement(8089..11328)
                     /locus_tag="PLB86_06765"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HRC64874.1"
                     /translation="MKPFGSEADWEDLALEDLGDLGWEPMGGKAIAPGSGERVSWDSL
                     VIPHRLQDALLRLNPEVPKHYLDQAMAEILAIRSGDALTENHRMHGILIGGYSGLSYV
                     DDEGVQRSPTVHVISANPDRNDWLAANQVRLVDRDHDRRFDVVLYCNGLPVGIIEVKK
                     GGAHHADVSAAHAQLETYVHEFPAEFRFALLTIATDGVISAYGTPFTPLNHFAPWNVD
                     DDGRPVEPGESQPDGSTPTSLSLLLYGVCNVVRILQLQRHFTAFHVDKDNGTLTKRIA
                     KPHQYFAVTKAVGSTIDAVRGDGRAGVVWHTQGSGKSMEMELYTYALSQRPELANPTV
                     VVITDRTDLDGQLFSSFARCELLRSTPVQISTRKQLREVLSETRSGGIYFTTLQKFGR
                     TLGERDAGETHPLLTDRSNVVVIVDEAHRSHYDDLDGYARHLRDALPRATLIAFTGTP
                     IARADHDTRAVFGDVIDVYDLTRAVKDGATVPVYFEPRLIRVALDDGVTPEDLDAAAN
                     EITLGLDDVERARIERSVANLNRLYGAPDRIEALVDDLLAHWDARAEAMTGFIGSRGK
                     AMIVCVTREVCVRVYEAIVRRRPDWANASDDKGRVKVVFSGVPSDPAAYQPHIRRPSQ
                     EKAVKERAANAQDELELIIVKDMLLTGFDSPSLHTLYLDRPMRDALLMQTLARVNRTF
                     RGKRDGLLVAYAPIADSLEHALGEYTVDDQQARPVGRNIAEAADAMIDLLSEFDACLV
                     GFPWRTRLAVGTPRAHVETVVAAANHLRSPANSTSDGADGDGADAEPVGLRFRRLAGQ
                     LSRLWALARGKESLTPEQQREVQFCEYVRVAMAKLDAEARRGRGEPIPEDIQLLLGKV
                     LDDSTDAGEILDIYAAAGLPRMSLAELTPSTMEAMRTAAKQADKAHLAIEQIRNLLLK
                     EAGSMVAGSLVRGRAFSERIDELMRKYTNQQLTAAEVMAALIELAKEVTADAARGAKF
                     TPPLGDDELAVFDALEANDSARLLQGEDTLAQIARELVVVMRRDIRTDWTVRDDVKAK
                     LRSSIKRLLVKYDYPPDQQQGAISLVMEQMEALAPRYAAERKAEGHAPS"
     gene            complement(11330..12541)
                     /locus_tag="PLB86_06770"
     CDS             complement(11330..12541)
                     /locus_tag="PLB86_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRC64875.1"
                     /translation="MARRLRQSIREIAAPAGLVGGPFGSNLVANDYCDQGIPVIRGSN
                     LGQGRYVSGDFAFVSQEKFERDLARNTATAGDIVFTQRGTLGQIALIAPSDLRTYVIS
                     QSQMRLRVDPKKANADFVYYAASTPEFQRLIRERAIATGVPHINLGILGELTLPLPSV
                     LTQQAIADVLGALDDKIASNALTTDSASALAAAEFQGAVRASSRVVRVADVSTLITRG
                     SAPRYVSEDKGLPILNQKCIRNGRVNRMLARSGDPSRIRKDTVLTRGDVLINSTGTGT
                     LGRVGRWTEDLPASLDSHVTLVRPAPHEGVNHVGYALLALEGVFESLAEGSTGQTELS
                     RASVAQTEIRMPSIVKPSLEELLDSLTRFADAATAQASMLTTLRDQLLPKLMSGELRV
                     EDAEKQVEEVV"
BASE COUNT         2041 a         4018 c         4479 g         2093 t
ORIGIN      
        1 cgacgaaccg cacgactgtc gagatcccgg tggcggcgac gagggccagc agcttgatgg
       61 gagtccctgc cgtcggccac ccgaggtcga ggagcgccag ccccccgctc gtcgcccccc
      121 aggtgagcag gaagaccacg aaaccctgcg cgtgctggcg ccacgcccga ccaccggtca
      181 cgcggaaggt ccaccgccgg ttgagggccg tgttgaccac cgtcgcgacg acgagcgcgg
      241 cgacgttggc gacctggctc ggccaggcga ggtgcagcag cgcgaacagg cccaggtgta
      301 cgaccgtgct caccgccccg accaccgcga atcggcccag ttggctcgcg agcccaatct
      361 gcgcgtctcc ggtcacggca ggcggggccg ccactgccag ggcgggttcg gtggccatgg
      421 tgcgcacgcc gacccttagg ccgtccgggt cgcggcaggc gtgctgccga cgcccccgga
      481 ggccggttgg gtgaggtcgt agaacgtcga gccgccgatt gtcaccgcac ggaagttggc
      541 cgacacccac gaggcgatcg acaaggattc gcggctgccg ccgttctgcc gcccgaaccc
      601 gccccccgcc gcgaagtagt ggatctgttt cgccgccaca tactcctgga actgcgccaa
      661 cgtcgggctc gggtccgacc cgttgaagcc gccgatggcc atgaccggga gttgggtgcc
      721 caactgcagg cccgcggcgt tctgtgagcc gacggccgct gcgacccacg tgtaggacga
      781 cgcgtcggcc gacagagcct ggacgaccgc ggccgagggc gtcgaggcgt tgagcagccc
      841 accgcccgca ccgccccggg aggtgccgcc gggcggcata ccccctggtg ctcgtcccgc
      901 accgccggag gtggcggggc cggcgaggac gatcgagccg gtgtggggag tggccacggt
      961 cgccacgctg tatgccgcgg ggccgagcaa cccggccgcg agggccactc ccagcacggc
     1021 gggaactgcc ttggcgggca gtcgatctgc gagcaggaag cccgcggcgg cggccaggcc
     1081 gagcgccagg acgacgacac gaagccagcc gttccacgag gtgacccgcg agagcaggac
     1141 aaagctccag acgccggtga tcaccgtgac cgcagccaac ggcaccgaac cgatccaccg
     1201 gtcgcggcgc tcccacgcct cggcgccgcc catcgccaca agggccgcga tcgctggagc
     1261 tagcgcgacg gtgtagtagg cgtgataaat gccggccatc agcgagaaga cgagggtggt
     1321 gatgacgagc cacgagcccc agacgaggta tgccgcgcgg cgagcgtcgg tgcgcggcat
     1381 acgcgcgcgg taggccagcc cgacgagcag gaggatgagc gccgcgggga gcagccacga
     1441 gatctggccg ccgttctcgg agttgaacat gcggcccagg ccgatctggg tcaggcgggc
     1501 cgaggcgttg ccgaccgagc cggtctcctc gccgttgatc cgaccgagtc cgttgtagcc
     1561 gaaggtcaac tcgaggaacg aattggtctg ggagccaccg atgtaggggc gcacgcttgc
     1621 gggaaggagc tcgacgaccg cgacccacca gccggctgac gcgacgatcg cgaggccccc
     1681 aatgacggtg tgccagatcc ggcgcagcaa cggaggtcgg gcggcgacga ggtaggccag
     1741 ggcgaagccg gggacgacga ggaaggcttg gagggtcttg gtgaggaagg ccaggccgat
     1801 gaagacgccg acgagggcga accacctcgg ggacgactca tcgatcgaac gcaccgtcgc
     1861 ccacgcggct agtgccatgg ccaagaccag cagcgcgtcg gggttgttga agcggaacat
     1921 caacaccgca accggggtga gggcgaggat ccccccagcg agcagccctg cggcagcgcc
     1981 gaattggcgc ttcacggtgg cgtagacgac gccgacagtg gcgaccccca tgagcacttc
     2041 aggcagcaga atcgcgaagg agctgaggcc gaagatccgc accgacaggg ccatgaacca
     2101 cagcgacgcg ggcggtttgt cgacggtgat cgcgttggcg gcgtcggaac tgccgaagaa
     2161 gaacgccttc cacgagtcgg ctccggcctg ggctgcggcg gagtagaacg cgttcgcgta
     2221 accgctggag gtgaggttcc acagatagaa gacggcggtc gccgcgagga ggccgagcag
     2281 ggcggggcgg acccaggacg ggtcacgtga ggaggttgcg gcagtggtgg cggtggtctg
     2341 gtcgggcccg gtggtcgagg tgggggccat ggcgagctcg gtcattcgac cacggtgatc
     2401 gttgccgcta tgggctacct gtgtggctgc aaggactggt ccgtgaggag gaggccaccg
     2461 gtggcacgcc tcgcgcgggg ggaggcgtgc caccggatgc gtcggcccgg attggtggat
     2521 cgatcagggg gtgacccgac cggcccaacg cggggtcgag gaggcgtcgt cgggcgcagg
     2581 cagcaccacg gagaagacgg tgtcgcccgg cgtgctcgtc aactgcaccg acccgccgtg
     2641 ggcactggca acagcggcca cgatcgacaa gcccaaacca gtgctcccgc cggtgcgaga
     2701 gcgggccgag tcgccgcgag cgaagcgttg gaagaccttg gcctgctggg cctcgggaac
     2761 cccaggcccg tcgtcgtgta ctgacaaccg cacggtgcct ggctcgcggc tgatgctcgt
     2821 gagcaccgtc gtgccgggca gggtgtgcac gcgggcgttg cccaggaggt tggcgacgat
     2881 ctgatgcagg cgggcgctgt ctccgcgcac aagcaccggc tcgtcgggca ggtccaggcg
     2941 ccacttgtgc tgcggcgagg cggcgtgggc gtcgctgacg gcgttgacga cgatctcggt
     3001 gaggtcgacg tcttcctggt cgagggggcg gccggcgtcc agccgggcca gcaggaggag
     3061 gtcgtcgacg agcgtcgaca tccgcttggc ctcggactcg acccggctga tcgcgtggac
     3121 cacccccgcg gggacgggct cgcgctcctt ccgggtcaac tcggcatacc ctctgatgga
     3181 ggcgagcggg gtccgtagct catgcgaggc gtcggcgacg aactggcgca cccgctgctc
     3241 actctcgtga cgcgcgttga gggcggagtc gacgtggtcg agcaactcgt tgagggcgag
     3301 gccgacccgg ccgacctccg tgcgcgggtc ggtgtcctcc tcggggaccc gttcggcgag
     3361 cgcgacctgc ccggacgaca ggtgcaactg cgagacgcgg gtggcggtgt cggcgacccg
     3421 ggtcagcggg cgcaggtttc gcgtgaccag ccaggcgccg ccgatgccga cggcgagcag
     3481 gccgagcacc gaggcgaaca gcgcggtccg gaaggcgttg ccgacggtgc cgacctggcg
     3541 gtcccggggg aggccgacgt agacgacggc gccgctgttg cgggccgagg tacccgcgag
     3601 gacccggtat tcgccgagtt cgccgccgag atcgatgttc cggggctggt tgcccagccc
     3661 ggcctcctgc agcagctgaa tctgggcgac cgtgatggcg tcgcgctggc gggtgtaggg
     3721 ggtgccgttg gcgacgacca cgacgaagcc ctcatcgccg ggcccgcggc cgaggtcccc
     3781 gtcgccgtcc cgcagggagg tcgacgacgc ggtccctccg agctggggcg ggatcttgct
     3841 gagtgaggaa cgcagttgat cgtccaactg ggcggtgagg gaacggtcga gggagagcac
     3901 cgatgccgcg gcgatgacga gggtcatcac cgtgaagagc accaacatcg aggcgacgag
     3961 cttgctgcgc agggtccagg tcgacgggtg ccacgagcgg gccgcggtgg tcggcgcggg
     4021 cgcgacgaac tcggcgggtg cggccgctac ggccgtcccg gcggctgctg cggggggttg
     4081 ctcagacatc agctcgccgg cttgaggacg tagcccacgc cacgcatggt gtggatcatt
     4141 ggctcgcggc cggcgtcgat cttcttgcgc aggtaggaga tgtagagctc gacgacgttg
     4201 gcctggccgc cgaagtcgta gttccagacg cggtcgagga tctgggcctt ggacagcacc
     4261 cgcttggggt tgcgcatgag gtagcgcagc aattcgaatt ccgttgccgt cagctggatc
     4321 tgggctccag cgcgggtgac ctcgtgggag tcctcgtcga gggtgaggtc gccgacgacc
     4381 aggcgcgagt cgtcctcctc ggtggccacc aggacggtcc gacgcatgag cgcgcgcagt
     4441 cgagcgacga cctcctcgag gctgaacggc ttggtgacgt agtcgtcgcc gcccgcggtg
     4501 agcccggcga tgcggtcttc gacggcgtcg cgtgcggtga ggaagagcac cgggaggcgg
     4561 tcgttgtgct cacgcatccg gcgcagcacc tccaggccgt ccatgtcggg aagcatcatg
     4621 tccagcgcga ccgcgtcggg ctcgaactcg cgcgcggctc ggacagccgc catgccggtg
     4681 ccggccgagc gcacctccca gccctcgtag cgcagggcca tcgacagcag ctcggtgagg
     4741 ttgtgctcgt catcgacgac gagcactcgc ggcggggtgc cgtcggggcg ctcgagcttc
     4801 tgcggcttga cggggttcgt ggtcatggtg gaagtctctc cgcggtgcgt gtcggcctcg
     4861 tgtgtgtttg ctgtgcgctt cctgtgagcg atcatcgcac tcacggcgca gcgacaggag
     4921 gccaacagcg tcgacacagt gcagcgtggc agcgtgggca gcccgcagca ccccagggcc
     4981 ttgatcggtc cgacgttgag atcagccgcg agcggccaac agtcccaacg gaattgacag
     5041 cacgacgagg gcacacaaaa gcccgagcag gatcgtcgtc cagtcacgca tccgcgcggc
     5101 ctgattggct ccgcggcgtc tccccacata tgccgtcatg gcacctagcc cccttgacac
     5161 tttgcttgat ccccagttgt gctgcctgcc ccgcgacccc cgcggaaccg gcgtggccga
     5221 cctgcgtcgg cggcataccc cgtgcgggtt ccctgcccgc ggaggagatt ctaccgccgt
     5281 tggcgccctt cggacagagc cctcagacgg cggggctgga cggcgtgcgc tggcaggcga
     5341 tcccgtggct gatcgggcga taaccgagga ggtcgttgac ggcgatcatg tgggtgttgg
     5401 actcggcgtt gctcgtcgtc agcatgccgc aggccggctc aacctcgccc aaccagcgca
     5461 ccatgtcggt cttgaggagc actccgaggc ggtgcccgcg gtgggcgtcc acaacggtcg
     5521 tgtcgtgttg gtcgccgaac gtcggccgtt cgggatcgac ggtgacgacg gtgtgaccgg
     5581 cgaactcccc ggtctcgcga tgtctggcga tgacccgata gagagtctcg ccgcgcacgg
     5641 cgtggcttcg ctcgtaggcg cgcacgcgct cggcgtcgaa gacctcgtcc tcctgctcga
     5701 ggtcgtcagt gggcgcgtca ttgatcgccg cgacggcctg cacgtagccg gccatgaggt
     5761 cgtccgggag ggctccgacg acgcgcagga ggtcgtagtc cgcggcatac gggagggctt
     5821 gggcgtatgc cgcctccgtc gcccccgcgg gaaggtcggc gaaggtgacg cgccgcgtga
     5881 cgctcctgga gcgcacctcg aaaccgatcg cgtcgacgaa cccgcgcgtg tgtggcaggt
     5941 cgaccccgaa ggtgcccagg acggtgcggc cggcgtcggt tgcgagcctg atgagctcgg
     6001 tgtaggtcca gcggccgatg ccctggcggc gggcgtcggg gtggacggcg gcgtagatcc
     6061 agccgctgtc gaggttgtcc cggctgggga gtgccatggc gccgacggcg acgagatcgc
     6121 tcccgcggcg gatgaggtag aacggctccg ggtcaccgtc atagccgagc aggtgctcct
     6181 ggcggatgct gcgcggggtc gcgccgtggc gccacggcgc atcgaccgcg tcggtggcgg
     6241 cgaagagtgc cacgatcgcg tcgagttgtc gctcgtcgtc cgggtcgagc agctgcaggt
     6301 ccagggtcat gccttccagg gtgaccctcg gtcgcgggcc gcgtcacctg gttttccgtc
     6361 gtcggtggct ggctcgttgt ggaaccggtc aggccgcagg aagccatccg ttgcgccgca
     6421 aggcctccca cgccacttcg acatggtccg tcgtgaacaa ccgacctttg cgagggctcc
     6481 actgctggac cgccgcagcg gcgatcttcg ggtcgcgggc agcccgggaa tcgtgcagag
     6541 ccacccagtg ggtgctcgcg agaagctcaa ggccataggc gctctcgaag ccgtcagcga
     6601 gggctaggac gcggctcatc cgctcgaggg tctcctcctg atgggtcagt gcggcatcaa
     6661 tgtcggtgtc gtggcccgag acaagggaga gcggctcggc ctcgttgact cgttgagagc
     6721 cgtcgccgaa gcccacgatg tactgccctt ccaactcgcg aagcacagcg cgcaggttgt
     6781 ctgcgtatgg gccgtagtga tgcctgacga acttcagttg cagggcttcc ccggcggcct
     6841 gaaggaagta catgagcttc tggacctcga tgaccgaggg agcctcgaac gtcgcctctt
     6901 gatacccgcg catcatcgcg aggagcgccg cgcggccacg tgtcaaggcg gcgacgggtc
     6961 ggttgtcgat catttccgca gccgagggtg ccccggctgg ggggtagacc cgcacttcga
     7021 cgccatcgag gtcacctagc gcaccgataa tcatgggccg tacgtcggcc caggcgagcc
     7081 cgccgttacc gcacccgagc ggcggcaccg cgaccgaggt gatcccgagc tcgctgatga
     7141 cccgccttag atcggccagg ccctcagcca cgtcgtcgat ccgcgacggg ctccgccagt
     7201 gccgcttggt ggggaagttg atgacgtagc gagggccgtc gagttggttg gtcggccaga
     7261 cgtgcatgtg tcccggctgc agctcgccgg ccttcgcggc cttctcgtat gccgtgaaca
     7321 tcgccgggta ggcgcgcttg aactgcaggg caatgccttt gcccatgacg ccggccgtgt
     7381 tgacggtgtt gaccagggcg tcgacgtccg cggtgagcag gttgcccgat acctcgcgga
     7441 tcatcgtcgt ctccatatgg ctagaaatac cagtcgggtc tgacagccgt ctgtacccag
     7501 gtgacgccca tgctagccag gaccccagcg gcctcgtcgc gaatggcgcc cgattttgcc
     7561 acgaggccaa ggatggcgtg aaaggggacg acctggtgga ccaggcactc ggccatccgt
     7621 cgctcccgtc gggagaggtc ttctggagtg ttcgcccagt aacgtgcgct catcaacggc
     7681 cagtccgtca tggcggcgat gtccccgtca tcgtcggcga acacggcaac ggactttagg
     7741 gggtgacggt ccgttccaag aacgttcagc ccgagctccc ggagtcgttg tgtggacgtc
     7801 accacataga tgagccgatc gcagccgtca gtgtaggtag ggactcggcc gtgatggatg
     7861 gaactcatca tggggctgcg aggcgcgtag tagaacggca cgtagtcggc gaccacgccc
     7921 ccgggcccaa cggggacgac gcgtcgaccc cgtccctcct tgattccggt gttgcccacc
     7981 tcgatctgca ggactcccgg ttcatgagct cgcgagtcac acaccaaacc gtgctggact
     8041 atcgaggcga ggtgctcgac atgcgtgaag tggcagaccc atgtgggctc aggacggggc
     8101 atggccctcc gccttgcgct cggcggcata ccggggtgcg agggcctcca tctgctccat
     8161 cacgagggag atggcgccct gctgctggtc tggggggtag tcatacttga cgaggagccg
     8221 cttgatcgac gagcgcagct tcgccttgac gtcgtcgcga acggtccagt cggtgcggat
     8281 gtcgcgacgc atgacgacga cgagttcgcg agcgatctgg gccagggtgt cctcgccctg
     8341 cagcaaacgg gcggagtcgt tggcctccag cgcatcgaag accgccaact cgtcgtcgcc
     8401 gagcggtggc gtgaatttgg ccccccgtgc ggcgtcggcc gtcacctcct tggccagctc
     8461 gatgagggca gccataacct cggccgcggt gagctgctgg ttggtgtatt tccgcatcag
     8521 ctcatcgatc cgctccgaga acgcccgacc gcgcaccagg ctcccggcaa ccatcgaccc
     8581 ggcttccttc agcagcaggt tgcgaatctg ttcgatcgcc aggtgggcct tgtcggcctg
     8641 cttggccgca gttcgcattg cctccatcgt tgagggagtc aactcggcaa gactcatccg
     8701 cggcagaccg gctgccgcat agatgtcgag gatctcgccg gcgtcggtcg agtcgtcgag
     8761 caccttgccc agcaacagct ggatgtcctc agggatcggc tcgccgcgac cccgtcgggc
     8821 ctccgcgtcg agcttggcca tcgccacccg gacgtactcg cagaactgga cctcccgctg
     8881 ctgctccggg gtcaatgact ccttgccgcg cgccagggcc cacagtcgac tcagctgtcc
     8941 cgccaatcga cggaaccgca gcccgaccgg ctcggcgtcc gccccgtcgc cgtccgcgcc
     9001 gtcggacgtg ctgttggccg ggctccgcag gtggttggcc gctgcgacaa cggtctcgac
     9061 gtgagctcgt ggcgtgccca cagccagtcg ggttcgccat gggaagccaa cgaggcaagc
     9121 gtcgaactcg ctcaggaggt cgatcatggc gtcggccgcc tcggcgatgt ttcgaccgac
     9181 cgggcgtgct tgctggtcat ccacggtgta ttcaccgagc gcgtgctcaa ggctgtccgc
     9241 gatcggagcg taggcgacga gcagcccgtc tcgtttgccc cggaaggtcc ggttgacccg
     9301 ggcgagggtc tgcatgagca gcgcgtcgcg cattggtcgg tccaggtaga gcgtatgcag
     9361 cgatggcgaa tcgaagccgg tgagcagcat gtccttgacg atgatgagct ccagctcatc
     9421 ctgggcgttg gccgcgcgct ccttgacggc cttctcttgg ctggggcgcc ggatgtgcgg
     9481 ttggtatgcc gcggggtcac tggggacgcc ggagaagacg accttgaccc ggcccttgtc
     9541 gtcggacgcg ttggcccagt ctggacgacg acgaacgatg gcctcgtaga cccggacgca
     9601 cacttcgcgg gtcacgcaca cgatcattgc tttgccccga ctgccgatga aacccgtcat
     9661 cgcctcggcg cgggcatccc agtgggcgag cagatcgtcg accagcgctt ctatccggtc
     9721 gggcgcgcca tacagccggt tgaggttggc gactgatcgc tcaatccgag cgcgttcgac
     9781 gtcgtccaaa cccagcgtga tctcgttcgc ggcggcgtcg aggtcttcgg gtgtcacgcc
     9841 gtcgtccagt gcgacacgga tgagcctggg ctcaaagtag accggaacgg tggcgccgtc
     9901 cttgacggct cgagtgaggt catagacatc gatgacgtcc ccgaagacag cacgagtgtc
     9961 gtggtctgcc cgtgcgatcg gcgtgcccgt gaaggcgatg agagtggccc ggggcagggc
    10021 gtcgcgcagg tggcgggcat aaccgtcgag gtcgtcgtag tgactgcggt gcgcttcgtc
    10081 gacgatcacg acgacgttgg aacgatcggt gagcagcgga tgggtttccc ccgcgtcgcg
    10141 ctcgccgagg gtccgtccga acttctgcaa cgtcgtgaag tagatcccgc cgctgcgagt
    10201 ctccgagagc acctcacgaa gttgtttccg tgtggagatc tgaaccgggg tgctgcggag
    10261 gagttcgcag cgcgcgaacg atgagaacaa ctggccatcc aggtcggttc ggtcggtgat
    10321 cacgacgacc gtggggttgg ccaactcggg ccgttgggag agggcgtagg tatagagctc
    10381 catctccatc gacttgcctg agccctgggt gtgccacacg acgccagctc gaccgtcgcc
    10441 gcgcacagca tcgatagtcg agccgaccgc cttggtgacc gcgaagtatt ggtgcggttt
    10501 ggcaatccgc ttcgtcagcg ttccgttgtc cttgtcgacg tggaaggcgg tgaagtgacg
    10561 ctgcaactgg aggatgcgca cgacgttgca gaccccgtag aggagcaggc tcaggctggt
    10621 cggggtgctg ccgtccggtt ggctctcgcc cggctcgacc gggcgcccgt cgtcgtcaac
    10681 attccagggc gcgaagtggt tgagcggggt gaacggcgtg ccgtaggccg agatgacgcc
    10741 gtctgtcgca atcgtgagca gcgcgaagcg gaactccgcc gggaactcgt ggacataggt
    10801 ctccaactgt gcgtgtgcag ccgaaacgtc ggcgtggtgg gcgccgccct tcttcacctc
    10861 gatgatgccg acaggcaggc cgttgcagta gaggacgacg tcgaagcgcc gatcgtgatc
    10921 gcggtcgacg aggcgcacct ggttggcggc gagccagtcg ttgcgatctg ggttggcgct
    10981 gatcacgtgg acggtcggcg accgctgcac gccttcgtcg tcgacatagc tgagcccgga
    11041 gtacccgccg atgagtatgc cgtgcatccg gtggttctcg gtcagcgcgt cgccggatcg
    11101 gatggcgagg atctcggcca tggcttggtc caggtagtgc ttggggacct cggggttgag
    11161 tcgcaacaac gcgtcctgga gccggtgggg gatgacgagg ctgtcccagc tcacgcgttc
    11221 gcccgaaccg ggagcgatgg ctttgccgcc catgggttcc cacccgaggt cgccgaggtc
    11281 ctccagtgcg aggtcctccc agtcggcttc actcccgaag ggcttcatct cacaccacct
    11341 cctcgacctg cttttcggcg tcctcaacgc gtagttcccc tgacatcagc ttgggcaata
    11401 actggtcccg gagggtggtc agcatcgatg cctgggctgt cgctgcatct gcaaaacgtg
    11461 tgagcgagtc gagcaattct tcgagagagg gcttgacgat ggacggcatt ctaatctcgg
    11521 tctgtgcgac agaggcccga gagagctcag tttgaccagt gctgccctcg gccagagact
    11581 cgaaaacgcc ttctagagcc aaaagcgcat atccaacatg attcactccc tcatgcggtg
    11641 ctggccgcac gagggtaacg tgggaatcga gggaagcagg caggtcctcc gtccatctgc
    11701 cgactcgacc taacgtacct gttccagtgg aattgataag aacgtccccg cgtgtcagga
    11761 ctgtatcttt gcgtatcctg ctcgggtctc cgctgcgggc cagcatgcga ttcacccgac
    11821 cgttgcgaat gcacttctga ttcagaatcg ggaggccctt atcctcgcta acgtatcgtg
    11881 gtgccgatcc gcgcgtaatc agagtgctga cgtctgcgac tctcactact cggcttgacg
    11941 ccctaacggc gccctggaac tcggccgcgg ccagcgccga agctgaatcc gtggtcaaag
    12001 cgttggacgc gatcttgtcg tcgagcgccc ccagaacgtc cgcgatcgct tgttgtgtga
    12061 gcacgcttgg tagcggaagt gtgagttcgc cgaggatgcc aaggttgatg tggggcacgc
    12121 ccgtggcgat cgccctctcc cggatgagcc tctgaaattc tggggtagat gcggcgtagt
    12181 agacgaaatc ggcgttcgcc ttcttgggat ctacccgcaa gcgcatctgg ctctgggata
    12241 tgacgtaagt gcgcaaatcg cttggggcga tcaacgcgat ctggcccagc gtgcctcgct
    12301 gagtgaacac gatatctccg gccgtggcgg tgtttcgcgc aaggtcccgc tcaaatttct
    12361 cttgagaaac gaatgcgaaa tctccgctaa cgtatcggcc ctggcccaag ttgctgccgc
    12421 gaatcaccgg gatgccttgg tcacagtagt catttgcgac gagattggac ccgaagggtc
    12481 cgccgacaag tccagccgga gcggcgatct cgcgaatact ctggcgaaga cgcctagcca
    12541 tcgatcctcc ccagttgctt gcggacgacc tgttcgaggc gggcggattc gtcgaagtgg
    12601 gcgaacaact cggcggtgag ccgttcgatc t
//
LOCUS       DAORPW010000062        12417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1065_length_12417_cov_858.369843, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000062 DAORPW010000000
VERSION     DAORPW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12417
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1065_length_12417_cov_858.369843"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PLB86_06775"
     CDS             <1..419
                     /locus_tag="PLB86_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRC64876.1"
                     /translation="SVTAGMAIFDTMQWVPNDVATVAMGMAASMGQFLLSAGTPGKRY
                     ATPHARVMMHQPSGGIGGTASDIKIQAEQMLHIKKQMAALIAEHTGQTLEQIERDSDR
                     DRWFTAEEAKAYGFVDHVVERASSAAHRADNPVTGN"
     gene            446..1093
                     /locus_tag="PLB86_06780"
     CDS             446..1093
                     /locus_tag="PLB86_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRC64877.1"
                     /translation="MTMNAGMAPMAPMGRYILPQFEERTSYGMKRLDPYTKMFEDRII
                     FLGVQVDDASADDIIAQLIVLESQDPDRDILMYINSPGGSFTAMTAIYDTMQYIRPDI
                     QTFVVGQAASAAAVLLGAGAPGKRYALPNARILIHQPALAGGDYGQASDIEIQANEVL
                     RMRTWLEETLALHTGRSAEQTARDIERDKILSSAEAKEYGLIDEVLLSRKGSAQK"
     gene            1283..2566
                     /gene="clpX"
                     /locus_tag="PLB86_06785"
     CDS             1283..2566
                     /gene="clpX"
                     /locus_tag="PLB86_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRC64878.1"
                     /translation="MARVGDGSDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELAETSELGLDELPKPREIFDFLGTYVVGQDIAKKALAVAVYNHYKRIQAGEGM
                     GRKGEDAVDIAKSNILLIGPTGCGKTYLAQTLARMLNVPFAIADATALTEAGYVGEDV
                     ENILLKLIQAADYDVKKAETGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILE
                     GTTASVPPQGGRKHPHQEFIQIDTTNVLFIVGGAFAGLEKLIEARSGKKGLGFGSQLH
                     LAKEEGWSYADVMPEDLMKFGLIPEFIGRLPVITTVTPLDRAALVAILSEPRNALIKQ
                     YQRMFEIDGVELEFTPEAVEAVAEQALLRGTGARGLRAIMEEVLQPVMFDVPSENDIA
                     RVVITREVVLDNVNPTIVRREAARVVKRAPRRQSA"
     gene            2675..3274
                     /locus_tag="PLB86_06790"
     CDS             2675..3274
                     /locus_tag="PLB86_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64879.1"
                     /translation="MAGAPAPQQLDGRTCGPTALTTARMLRDPALADWVRTGDKHGRA
                     FPNGPSAALRLAAYHSLVHARTNALVGPGGRLQVPWPRALGTTPWGVCRELESGAADP
                     GARYRIVPVRRADGSRLAALVGHLASHLRPGRPGVLFVGSATLPRHVALLVAGPDGSV
                     LVHDPSAGSVSELDVASLADPRTAVAGWTHPWFLIGPVG"
     gene            complement(3308..5935)
                     /gene="valS"
                     /locus_tag="PLB86_06795"
     CDS             complement(3308..5935)
                     /gene="valS"
                     /locus_tag="PLB86_06795"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRC64880.1"
                     /translation="MTSTPLRPTSIPDRPSVDGLEEKWGAVWREEGTYTFDRAKAMAG
                     PRSAVFSIDTPPPTASGSLHMGHVFSYTHTDCMARYKRMAGFEVFYPIGWDDNGLPTE
                     KRVQNYYGVRGDASLPYIPDFQPPHQGDVASIKAANQIAISRQNFIELCDVLTVLDEE
                     AFEALFRRMGFSLDWSISYRTIDDHSRATAQQAFLRNLERGEAYTSEAPGLWDVTFQT
                     AVAQAELEAKDYPGAYHRVAFHRPDGSPVYIETTRPELIPACVALIAHPDDERYQPLF
                     GTTVTSPLFGVEIPVRAHPAAEPDKGAGIAMCCTFGDLTDVIWWRELQLPNRSLITRS
                     GRLDAETPDWLSGTGAELYAAKIAGATAYAAREATVAALRESGDLDGEPTPTMRKANF
                     YERGEKPLEIVSSRQWYIRNGGRDAALNAQFVDRGREIAFHPGFMRSRYENWVQGLNG
                     DWLISRQRFFGVPIPVWYRLDDQGEPVYDTPLVPREDQLPLDPAASVPEGFTAEQRGI
                     PGGFVADPDVMDTWATSSLSPQIAAGWRGDPDLFATVFPMDVRPQAHDIIRTWLFSTI
                     VRAHYEHGTVPWTNAAISGWILDPDRKKMSKSKGNVVTPEALVQEHSADAVRYWAASG
                     RLGTDAAFDAGQIKVGRRLAIKILNASKFALSFGTVEGDLGAAVTEPLDRSMLSALAD
                     VVRSATAGFEAWDYTRSLEITESFFWTFCDDYLELVKDRAYGGQGEAAAASARAALRI
                     ALDVLLRLLAPILPYVTEEVWSWWQEGSVHRQPWPTLADVVGAQAADGTSGDPEVLTA
                     VGAALAAVRKVKSEAKMGMRAEVASMTLAVPTEVGERVVLAEADLRAAGRITAMTYAV
                     SDAVEVRDAQLIPVEKPTA"
     gene            6123..6662
                     /locus_tag="PLB86_06800"
     CDS             6123..6662
                     /locus_tag="PLB86_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC64881.1"
                     /translation="MTTTVPAPALPGTREWRLGLPAWIHDARGAELLLRFVTPADAGA
                     IRGLHARCSEQALRWRYFGGGAQVEHLLTWIFDETRSEAVTAWSQGQLVAVGNLLLPD
                     ASGVGEVAFLVEDAWQGRGIGSTLVDVVTDRGRDHGAGLLHADVSLDNGRMRVLLARR
                     GWVGKVVGGDYCMDLVLSD"
     gene            complement(6632..7429)
                     /locus_tag="PLB86_06805"
     CDS             complement(6632..7429)
                     /locus_tag="PLB86_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRC64882.1"
                     /translation="MFRHAPAGDLAGLVRHIWVPVWDIPAGEEWEQHVLNYPCCQVVV
                     GSDYARFYGVVRGLSRVTLAGRGWVVGIGLQPGAGWLVAGRPVSALTDTWVDLDEMGM
                     PWSALVPAVRSVMSPDPSDALAQRRAAGILEDAVRRHVVIDEEGLLVNAIVDAVESDQ
                     SLLRVDQLAARFALTERSLQRLTRRRLGLTPRWLVQRRRLHEAADRLRRGETDLAGLA
                     AYLGYADQAHLSREFKAVTGHTPATFRATATRADPAGDQSESTRSMQ"
     gene            7585..8130
                     /locus_tag="PLB86_06810"
     CDS             7585..8130
                     /locus_tag="PLB86_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HRC64883.1"
                     /translation="MTTTTVTPYRAALTPLSDIVATVTDWAAPSPCEGWSALDVLDHI
                     LTTQRALLTERGLTLPPPADLAADPVAAWAAHAATVAGLLDDPAIAGIAYDGHFGPTT
                     LGETVERFYVFDMLVHRWDLARAAGSDTSFTDAELDRIDESAQSFGPVLYMEGICRPA
                     VPAASDADRQTRVLATLGRRA"
     gene            8186..9004
                     /locus_tag="PLB86_06815"
     CDS             8186..9004
                     /locus_tag="PLB86_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC64884.1"
                     /translation="MDHVYFPVIGIAKTLFALQGLRFTVTGAQNVPKHGGAVLAINHT
                     GYLDFTYAGNAVLPRGRFVRFMAKESIFKNPVAGPLMKGMKHIPVDRAAGGAAYRRAV
                     AALRDGEIVGVYPEATISRAFELKDFKVGAARMARDAGVPIIPCVIWGAQRVWTKDHP
                     KRMGRTNTPIYVDLGTPIDVPPEANPHEVTAQLKATMTAMLEGLWDAYPPVPEDEQVF
                     VPKSRGGQAPTLEEAAELYKQELARKAAKRAAKAAAQDSGQDSGQDSGGATAKG"
     gene            9047..9823
                     /locus_tag="PLB86_06820"
     CDS             9047..9823
                     /locus_tag="PLB86_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HRC64885.1"
                     /translation="MRQREPEGPPPAPAVQKLRIRYAKRGRLRFSSTRDFQRALERAL
                     RRADVPMAFSGGFHPHPRISYANAAATGTASEAEYVEISVTARVDPDALRLALDAALP
                     DGLDVLAVVEAGPGALADRLQASEWVIALRGMPVGQLREAADAFWAQTQVEVTRVLKA
                     GPRTFDARAAVLDLAVDTAASAPVMTGVVPGECAILRLVVRHTTPAVRPDDVLTALRV
                     VAGLEPPTPPLVIRLAQGPLQEGSVRVTDPLAASESDAGV"
     gene            9984..>12417
                     /locus_tag="PLB86_06825"
     CDS             9984..>12417
                     /locus_tag="PLB86_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRC64886.1"
                     /translation="MASTEDQSDPTTAVVGAPEPQASDASDAPAGAATRAARPRRSRA
                     KAAAPQAQAEPSDVTSAAGQPSDGAAGADAGAPADGAAADAGPAPAKKVARRSRKAAA
                     APVAAPAAADAAATDGDAPDAAAQAALAVQTAPAADPIVAAPRKRASRRVAAPTATPE
                     SADGTGSADGAAPTDGATAGEIAASADEVAGGAAPAAATTRTPRKRAPRKTAASAAAA
                     TAAPAPIAETGDPALDEEAAEAAAEAEAAEEATRAATVPSFGLLFQAPVVVTPRRRAT
                     RPSGTPALPVAAADAEAQDTLVPADEPEADDIEVGAADADVLTDTDADDAVDDATSPA
                     ARRRRRRGGKGRRGRLTDGPSTAGEGAAAAPAPAAGDAPDTATASGSADGSGDEGDGD
                     TDVRSDLASRRRTRRRRRGSSAGEVEDAPGVVTRVREVRSARDEVVGVRGSTRLEAKK
                     QRRREGREAGRRRQVITEAEFLARRESVERVMVVRAKEGRTQIGVLEDNVLVEHYVSR
                     ETNASMAGNIYLGKVQNVLPSMEAAFVDIGRGRNAVLYAGEVNWDAAGMEAGASKRIE
                     NALESGQSVLVQVTKDPIGHKGARLTSQISLPGRYLVYVPDSSMTGISRKLPDTERAR
                     LKRILRDVVPESAGVIVRTAAEGASEEELRADVERLTAIWEQIKAKAEAAGSGKKGKG
                     NSPVLLHGEPDLTVRVIRDVFNEDFAQMIVAGDEAWAQINDYVGSVAPDLASRLTKWT
                     GERDIFSNYRIDEQLAKAMDRKVWLPSGGSLVIDRTEAMTVIDVNTGKFVGAGGNLEE
                     TVTRNNIEAAEEI"
BASE COUNT         1945 a         4390 c         4325 g         1757 t
ORIGIN      
        1 ggtcggtgac cgccggcatg gcgatcttcg acaccatgca gtgggtgccc aatgatgtgg
       61 ccaccgtcgc gatggggatg gcggcctcga tgggccagtt cctcctctcg gccggcacgc
      121 cgggcaagcg ctacgccacg ccgcacgccc gagtgatgat gcaccagccc tccggtggca
      181 tcggcggcac ggcctcggac atcaagatcc aggccgagca gatgctgcac atcaagaagc
      241 agatggccgc gctcatcgcc gagcacaccg gccagaccct cgagcagatc gagcgtgact
      301 ccgaccgcga ccgctggttc accgcggagg aggccaaggc ctatggcttc gtcgaccacg
      361 tcgtcgagcg ggcctcgagc gcggcacacc gcgccgacaa ccccgtgacc ggcaactgac
      421 gcccgagacg acggagagac cgaccatgac catgaacgca gggatggccc cgatggcccc
      481 gatgggccgc tacatcctgc cccagttcga ggagcgcacg tcctacggga tgaagcgcct
      541 ggacccctac acgaagatgt tcgaggaccg catcatcttc ctgggtgtcc aggtcgacga
      601 cgcctcggcc gacgacatca tcgctcagct catcgtgctg gagtcccagg accccgaccg
      661 cgacatcctc atgtacatca acagccccgg tggctcgttc acggcgatga ccgcgatcta
      721 cgacaccatg cagtacatcc ggcccgacat ccagacgttc gtcgtcgggc aggccgcctc
      781 ggccgctgcg gtgctgctgg gtgccggcgc gccgggcaag cgctacgccc tgcccaacgc
      841 gcgcatcctc atccaccagc cggccctcgc cggtggtgac tacggccagg cctccgacat
      901 cgagatccag gccaacgagg tgctgcggat gcgcacctgg ctggaggaga cgctggccct
      961 gcacaccggc cgctccgccg agcagacggc ccgcgacatc gagcgcgaca agatcctcag
     1021 ctcggccgag gccaaggagt acggcctcat cgacgaggtt ctcctctcgc gcaagggctc
     1081 cgcacagaag tgacctacgc accggtatgc cgcccgctca cccaggcgcg cggcataccg
     1141 gtgtcgtttg tccgccctca gcggacaatc gctgacgccg ggtggatccg ggcgtgtaat
     1201 gaagagcgca accggcgtgg ggcaggtagc gtcggccttg gacctgaccg cattgggcgg
     1261 tcacgacagc aggaggaccc agatggcgcg cgtgggtgac ggcagcgatc tgctcaagtg
     1321 ctccttctgc ggcaagagcc agaagcaggt caagaagctc atcgcgggcc ctggcgtcta
     1381 catctgcgac gagtgcatcg atctgtgcaa cgagatcatc gaggaggagc tcgccgagac
     1441 ctccgagctg ggcctggacg agctgcccaa gccgcgcgag atcttcgact tcctcggcac
     1501 ctacgtcgtc ggccaggaca tcgccaagaa ggcgctcgcg gtcgcggtct acaaccacta
     1561 caagcggatc caggcggggg aggggatggg ccgcaagggt gaggacgcgg tcgacatcgc
     1621 caagtccaac atcctgttga tcggccccac cgggtgcggc aagacctacc tcgcccagac
     1681 cctggcccgc atgctcaacg tgccgttcgc catcgccgac gcgacggcgc tgacagaggc
     1741 cggatatgtc ggcgaggacg tcgagaacat cctcctcaag ctcatccagg ccgccgacta
     1801 cgacgtcaag aaggccgaga cgggcatcat ctacatcgac gaggtcgaca agatcgcccg
     1861 caagagcgag aacccgtcga tcacccgcga cgtctccggt gagggcgtgc agcaggcgct
     1921 gctgaagatc ctcgagggca cgaccgcctc ggtgccgccg cagggtggcc gcaaacaccc
     1981 gcaccaggag ttcatccaga tcgacacgac gaacgtcctg ttcatcgtcg gcggcgcttt
     2041 cgccgggctc gagaagctca tcgaggcacg ctcgggcaag aagggcctgg gcttcgggtc
     2101 gcagttgcac ctggccaagg aagagggctg gtcctacgcc gacgtcatgc ccgaagacct
     2161 gatgaagttc gggctcatcc ccgagttcat cggccggctt ccggtcatca cgacggtgac
     2221 tccgctggac cgggcggccc tcgtcgcgat cctcagcgag ccgcgcaacg cgctcatcaa
     2281 gcaataccag cggatgttcg agatcgacgg cgtcgagctc gagttcaccc ccgaggccgt
     2341 agaggcggtg gccgagcagg ccctgctgcg cggcaccggc gcccgtgggc tccgggcgat
     2401 catggaggaa gtcctccagc cggtgatgtt cgacgtcccc agcgagaacg acatcgcccg
     2461 ggtcgtcatc acccgcgagg tcgtgctcga caacgtcaac ccgacgatcg tgcgccgcga
     2521 ggccgcccgc gtggtcaagc gcgcaccgcg ccgccagtcc gcctgagcat tcgcccgccc
     2581 gcggtcgccg ctcacagccc tcatgcccgc cttcggctga cgtcgggtcg gtgtcccggt
     2641 ggatcgtgat tcgcagcccc ttgggcttca actcgtggcc ggggcccccg cgccgcaaca
     2701 gttggacgga cgcacctgcg ggccgacggc gttgacgacg gcccggatgc tgcgcgatcc
     2761 cgccttggcc gattgggtcc gcaccgggga caagcacggt cgagcctttc ccaacgggcc
     2821 gagcgcggcg ttgcggctgg cggcatacca ctcgctcgtc cacgcccgca cgaacgcgct
     2881 cgtcggcccc ggcgggaggc tgcaggtccc gtggccgcgc gctttgggca ccactccctg
     2941 gggggtatgc cgtgagctcg agtcgggcgc ggccgacccg ggtgcgcgct atcggatcgt
     3001 gccggtgcgc cgggcggatg ggtctcgcct ggccgcgctg gtcgggcacc tggcatcgca
     3061 tctgcgccca ggccgacccg gtgtgctctt cgtcgggagc gccaccttgc cccggcacgt
     3121 cgcgttgctc gtcgccggtc ccgacggctc ggtgctggtg cacgacccta gcgccgggtc
     3181 cgtgagcgag ctagacgtcg cgtcgctggc cgatccacgc acggccgtcg ccgggtggac
     3241 ccacccgtgg ttcctcatcg gccccgtcgg ctgacgacag ggccgatgcg ccgaccggac
     3301 cgacgggtca ggcggtgggc ttctccacgg ggatgagctg ggcgtcgcgg acctcgacgg
     3361 cgtcggacac ggcatacgtc atcgcggtga tgcgtcccgc ggcgcgcagg tcggcctcgg
     3421 cgagcacgac ccgctcgccc acctcggtcg gcacggcgag ggtcattgag gccacctcgg
     3481 cgcgcatccc catcttggct tcggacttca ccttgcggac ggcagcgagc gcggcgccga
     3541 cggcggtgag gacctcgggg tcacccgacg tcccgtccgc cgcctgcgcg cccacgacat
     3601 cggcaagcgt cggccagggc tgccggtgta ccgaaccctc ctgccaccac gaccacacct
     3661 cttcggtgac gtagggcagg atcggcgcga gcagccgcag gagcacgtcc agcgcaatcc
     3721 gcagggcggc ccgagccgac gcggccgccg cctcgccctg ccccccgtag gcccggtcct
     3781 tgaccaactc gaggtagtcg tcgcagaacg tccagaagaa cgactccgtg atctccagcg
     3841 accgggtgta gtcccaggcc tcgaatcccg ctgtcgcaga acgcaccacg tcggccagcg
     3901 ccgagagcat cgaccggtcg agcggctcgg tcaccgcggc gccgaggtca ccctcgacgg
     3961 tcccgaacga caaggcgaac ttgctggcgt tgaggatctt gatcgccagg cgccgaccca
     4021 ccttgatctg cccggcgtcg aaggcggcgt ccgtcccgag ccgcccggag gccgcccaat
     4081 agcgcaccgc gtcggccgag tgctcctgga ccagcgcctc cggcgtgacg acgttgccct
     4141 tggacttgct catcttcttg cgatcagggt cgaggatcca gcccgagatc gccgcgttgg
     4201 tccacggcac ggtgccgtgc tcgtagtggg cgcgcacgat cgtcgagaac agccaggtgc
     4261 ggatgatgtc gtgagcctgc gggcgcacgt ccatggggaa gaccgtcgcg aacaagtcgg
     4321 ggtcgccacg ccagccggcc gcgatctggg gggagagcga cgaggtcgcc cacgtgtcca
     4381 tcacgtcggg gtcagcgacg aaaccccctg gtatgccgcg ctgctcggcg gtgaagccct
     4441 ccggcacgga tgcggcgggg tcgagcggga gctggtcctc tcggggtacg agaggggtgt
     4501 cgtagaccgg ctcaccctga tcgtcgagtc gataccagac ggggatcggc acgccgaaga
     4561 accgctgccg cgagatgagc cagtcaccgt tgaggccctg cacccagttc tcgtagcggc
     4621 tgcgcatgaa gcccgggtgg aacgcgatct cgcgcccccg gtccacgaac tgcgcgttga
     4681 gcgccgcatc gcggccaccg ttgcggatgt accactggcg cgacgacacg atctccagcg
     4741 gcttctcacc ccgctcgtag aagttggcct tgcgcatcgt cggggtcggc tcgccgtcga
     4801 ggtcgccgct ctcgcgcagt gccgcgacgg ttgcctcacg cgcggcgtat gccgtcgcgc
     4861 cggcgatctt cgcggcatac aactccgcac ccgtccccga caaccagtcc ggcgtctcgg
     4921 cgtcgaggcg tccggaacgg gtgatgaggg agcggttggg caactgcagt tcccgccacc
     4981 agatgacgtc ggtgaggtca ccgaaggtgc agcacatcgc gatgccggcg cccttgtcgg
     5041 gctcggccgc cgggtgggcg cggaccggaa tctcgacgcc gaagagcggc gaggtgaccg
     5101 tcgtgccgaa caacggctgg taacgctcgt cgtcggggtg ggcgatgagg gccacgcagg
     5161 ccgggatgag ctcgggccgg gtcgtctcga tatacaccgg cgacccgtcg ggccggtgga
     5221 aggcgacccg gtgataggcg ccggggtagt ccttggcctc gagctcggcc tgggccaccg
     5281 ccgtctggaa cgtcacgtcc cagagcccgg gtgcctccga cgtgtaggcc tcgccgcgct
     5341 ccaggttgcg caggaacgcc tgctgggccg tcgcccgcga gtggtcgtcg atcgtgcggt
     5401 aggagatgga ccagtcgagc gagaacccca tccgccggaa gagtgcctcg aaggcctcct
     5461 cgtcgaggac ggtcaacacg tcgcacaact cgatgaagtt ctgccggctg atggcgatct
     5521 ggttggccgc cttgatcgag gcgacgtcgc cctggtgcgg cggctggaag tcggggatgt
     5581 agggcagcga cgcgtcacca cgcacgccgt agtagttctg cacccgcttc tcggtcggca
     5641 ggccgttgtc gtcccacccg atcgggtaga agacctcgaa cccggccatc cgcttgtaac
     5701 gggccatgca gtcggtgtgc gtgtaggaga acacgtgacc catgtgcagg ctgcccgagg
     5761 ccgtgggcgg cggcgtgtcg atcgagaaga cggcgctgcg cggcccggcc atggccttgg
     5821 cccggtcgaa cgtgtaggtc ccctcctcgc gccataccgc gccccacttc tcctcgaggc
     5881 cgtcgaccga ggggcggtcg gggatcgagg tcgggcgaag cggcgtgctg gtcatgccag
     5941 gcatcttctc agaaccggca ggagcctccg aaccggtttc ggtgtggacc gtccggcggg
     6001 tgtgatcggg cccaccagtg gcgagggtcg caggtccctg gaccgggccg tgagagcggt
     6061 tacgttaccg agtggtagca ggctgctcgc ctgtcgcccg ccgatcaacg aggaccagcg
     6121 ccatgacgac cacagttccg gccccggccc tgccggggac ccgcgagtgg cgccttggcc
     6181 tgccggcctg gatccacgac gcgcggggcg ccgagttgtt gttgcgcttc gtcacgcccg
     6241 ccgacgcggg agcgatccgg gggctgcacg cgcggtgttc ggagcaggcc ctgcggtggc
     6301 gctatttcgg gggcggtgct caggtcgagc acctgctcac ctggatcttc gacgagaccc
     6361 gcagcgaggc cgtcaccgcc tggagtcaag ggcagctcgt cgcggtgggc aacctgttgt
     6421 tgcccgacgc gtcgggtgtc ggcgaggtgg ccttcctcgt cgaggacgcc tggcagggtc
     6481 gggggatcgg atcgacgctg gtcgacgtcg tcaccgaccg gggccgggac cacggtgcag
     6541 gcctgctgca tgccgacgtg tcgctcgaca acggccggat gcgggtgctg ttggcccggc
     6601 gcggctgggt cggcaaggtc gttggtggcg actactgcat ggacctcgtg ctctccgact
     6661 gatcgccggc cgggtcggcc cgggtggccg tcgcgcggaa ggtcgcggga gtgtgcccgg
     6721 tcacggcctt gaactctcga ctcaggtgcg cttgatcggc ataccccagg tatgccgcca
     6781 gccccgcgag gtcggtctcg ccgcggcgca ggcggtcggc cgcctcgtgc aggcgtcggc
     6841 gctggaccaa ccaccggggc gtcaacccca ggcgccgacg ggtcaaccgc tggagggagc
     6901 gctcggtgag ggcgaagcgg gcggcgagtt ggtcgacccg cagcagcgac tggtcgcttt
     6961 cgaccgcgtc gacgatggcg ttgaccagca gcccctcctc gtcgatgacc acgtgccggc
     7021 gaacggcgtc ttcgaggatt ccggccgccc gccgttgggc gagggcgtcg gacgggtcgg
     7081 gggacatcac cgaccggacg gcagggacca gggccgacca cggcataccc atctcgtcga
     7141 ggtccaccca cgtgtcggtg agtgccgaga cgggtcggcc agcaacgagc caccccgcgc
     7201 ccggttgcag accgatgccc accacccacc cgcgcccggc cagggtcacc cgggacagac
     7261 cacgcactac gccgtagaag cgggcgtagt cggagcccac caccacctga caacaggggt
     7321 agttgagaac gtgttgctcc cactcctcgc cggctgggat gtcccagacg ggcacccaga
     7381 tgtggcggac cagtccggcg aggtcgcccg ccggagcatg gcggaacacc gggggactcg
     7441 gccggccctg cacgcgcagg tgcgccttgg ctaccgggtg atccggggac tgctcggatg
     7501 cggacggctg agggcggctg ccgatgtcgg tttgtcggga atcttcaaga ccggtcagtg
     7561 ccactccctt cacgcttggg cgtcatgacg acaaccaccg tgacaccgta ccgagccgcc
     7621 ctgacacctc tcagcgacat cgtcgccaca gtcaccgact gggctgcgcc gtcgccgtgc
     7681 gaggggtgga gcgccctgga cgtcctggac cacatcctca cgactcagcg tgctctgctc
     7741 accgagcgcg gcctgaccct gcccccgcct gctgacctcg cggccgaccc cgttgcggcc
     7801 tgggccgccc atgccgccac ggtggcgggg ctgctggatg acccagccat cgccgggatc
     7861 gcctacgacg gacacttcgg gccgaccacg ctcggggaga cggtggaacg cttctacgtg
     7921 ttcgacatgc tcgtccaccg ttgggacctg gctcgggcgg ccggcagcga cacgtccttc
     7981 accgacgctg aactcgaccg gatcgacgag tcggcgcagt cgttcgggcc ggtgctctac
     8041 atggagggca tctgccgacc ggccgtgccg gccgcgtcgg acgccgaccg gcagacccga
     8101 gtgctcgcca cgttgggccg ccgcgcgtga ggtgagcggg cggcatacca gcgggtatgc
     8161 cgcccaccca cctacagtgt ggggcgtgga ccacgtctac ttccccgtca tcgggatcgc
     8221 caagaccctc ttcgccctcc aggggctgcg tttcaccgtg accggtgcgc agaacgtgcc
     8281 caaacacggg ggagcggtgc tggcgatcaa ccacaccggc tatctcgact tcacctatgc
     8341 cggcaacgcc gtgctgcctc gcgggcggtt cgtcaggttc atggccaagg agtcgatctt
     8401 caagaacccg gtggccggcc cgctgatgaa ggggatgaag cacattcccg tcgaccgcgc
     8461 ggcgggcggg gcggcatacc gtcgggcggt cgctgcgctg cgtgatggtg agatcgtcgg
     8521 ggtctatccc gaggcgacca tctcgcgggc gtttgagctc aaggacttca aggtgggcgc
     8581 cgcgcggatg gcgcgcgacg ccggtgtccc gatcatcccg tgtgtcatct ggggagccca
     8641 gcgggtgtgg accaaggatc acccgaagcg gatgggccgc accaacaccc ccatctacgt
     8701 cgatctcggc accccgatcg atgtcccgcc agaggcgaac ccgcacgagg tgacggccca
     8761 gctcaaggcg accatgacgg cgatgctcga agggctctgg gacgcctacc cgccggtgcc
     8821 ggaggacgag caggtcttcg tgcccaagag tcgtggcggg caggcgccaa ccctcgaaga
     8881 agccgcagag ctctacaagc aggagttggc ccgcaaggcg gccaagcgcg cggccaaggc
     8941 cgcagctcag gactcaggtc aggactcagg tcaggactcg ggcggcgcga cggcgaaggg
     9001 ctgacacggc atacggcaga gacggcatac tctgtccgcc gtgggaatgc gacagcggga
     9061 gcccgagggg ccacctccgg caccggcggt gcagaagttg cggatccggt atgccaagcg
     9121 cggccggttg cgcttctcgt ccactcgcga cttccagcgg gcgttggagc gggcgctgcg
     9181 gcgagccgat gtgccgatgg cgttctccgg cggcttccac ccccacccgc ggatctctta
     9241 cgccaacgcg gcagctacgg ggacagcgag cgaagctgaa tacgtcgaga tctccgtcac
     9301 tgcgcgcgtc gaccccgacg cgctgcgcct cgccctcgac gcggccctcc ccgacggcct
     9361 ggacgtcctc gccgtcgtcg aggccggtcc cggcgcgctc gctgaccgcc tccaggccag
     9421 cgagtgggtc atcgccctga ggggtatgcc ggtgggccaa ctccgcgagg ccgctgacgc
     9481 gttctgggcg cagacccagg tcgaggtgac gcgcgtgctc aaggccgggc ctcggacttt
     9541 cgatgcccgc gctgcggtcc tggacctggc ggtcgacacg gcagcgtcgg caccggtcat
     9601 gacaggcgtc gtgccaggag agtgtgcgat actccgcttg gtcgtacggc acaccacacc
     9661 ggctgtacgc cccgacgacg ttctgaccgc gctgcgtgta gttgctggtc tggagccgcc
     9721 gacaccgccc ctggtgatcc ggctggccca aggccccctg caagagggca gcgtgagggt
     9781 gaccgatcca ttggccgcca gcgagagtga cgccggcgtc tgactgggtg ccgcgcatcc
     9841 cgcgcgaacc gatcaggcgt agcagagacg acttccgtcc tgccgcatcc gcggcggtga
     9901 cgacgcagac cgcgccacgc ggtgacgaag ggcctggccc tggcggtgtg ggcgtccccg
     9961 cgtgggtgac gagaggacgc cgcatggcat ccaccgaaga ccagtcagac ccgaccaccg
    10021 ccgtcgttgg cgccccggag ccgcaggcgt ccgacgcctc cgacgccccc gcaggtgcgg
    10081 cgacccgcgc agcgcgaccg cgccgcagcc gcgccaaggc ggccgccccg caggcccagg
    10141 ccgagcccag tgacgtgacg tcagcagccg ggcagccctc cgacggggcg gctggcgctg
    10201 acgccggtgc ccccgctgat ggcgcggcag ccgatgccgg accggcgccg gccaagaagg
    10261 tcgctcgacg cagccggaag gcagcggccg ctccggtcgc tgctcccgct gctgctgacg
    10321 ccgccgccac agacggcgac gcccccgacg cagccgctca ggcggcgctc gccgtgcaga
    10381 cggcacccgc ggcggacccg atcgtcgcgg cgccccgcaa gcgggcctcg cgtcgggtcg
    10441 ctgctccgac ggccactccc gagtctgcgg atggcactgg ctcggccgat ggcgctgccc
    10501 cgaccgacgg tgctaccgcc ggcgagatcg ctgcttcagc tgacgaggtc gcgggcgggg
    10561 ccgctccggc cgctgccacg acccggaccc cgcgcaagcg ggctcctcgc aagaccgccg
    10621 catcggccgc tgcagcgacc gcggcacccg cgccgatcgc tgagaccggc gacccggccc
    10681 tcgacgagga ggcagccgag gccgcagccg aggcggaggc agcggaggaa gcaacccggg
    10741 cggccacagt gccgagcttc ggactgctct tccaagcacc ggtcgtcgtc accccgcggc
    10801 gtcgtgccac ccggccgagc ggaactcccg cgcttccggt tgccgccgct gacgctgaag
    10861 cccaggacac cctggtcccg gccgacgagc cggaggcaga cgacatcgag gtcggcgccg
    10921 ccgacgctga cgtcctgacc gataccgatg ccgatgacgc cgtcgacgac gccaccagcc
    10981 ccgccgcccg tcggcgtcgg cgccggggtg gcaagggccg tcgcggtcgc ctgaccgacg
    11041 gcccgagcac cgcgggcgag ggcgccgcgg ctgcaccggc acccgccgct ggcgacgcac
    11101 cggacaccgc gacggccagc gggtcggctg acggatccgg cgacgagggc gacggtgaca
    11161 ccgacgtccg cagcgacctg gccagccgcc gccgcacgcg tcggcgccgt cgtgggtcca
    11221 gcgccggcga ggtcgaagat gctcccggcg tggtcactcg cgtccgcgag gtccgctccg
    11281 cccgcgacga ggtcgtcggt gtccggggtt cgacccgtct ggaggccaag aagcagcgtc
    11341 gtcgcgaggg ccgcgaggcc gggcgtcgcc gtcaggtcat caccgaggcc gagttcctcg
    11401 cccgccgcga gagcgtcgag cgggtcatgg tcgtgcgggc caaggaaggc cgcacccaga
    11461 tcggcgtcct cgaggacaac gtcctcgtcg agcactacgt ctcccgcgag accaacgcgt
    11521 cgatggcggg gaacatctac ctcggcaagg tccagaacgt cctgccctcc atggaggccg
    11581 ccttcgtcga catcgggcgt ggccgcaacg ccgtcctgta tgccggtgag gtcaactggg
    11641 acgcagcggg catggaggcc ggtgcctcca agcggatcga gaacgccctg gagtcgggtc
    11701 agtcggtcct cgtccaggtg accaaggacc ccatcgggca caagggcgcg cggctcacct
    11761 cgcagatctc cctgccgggc cgttacctcg tctacgtccc ggacagctcg atgaccggca
    11821 tctcgcgcaa actgcccgac accgagcggg cccggctcaa gcggatcctg cgtgacgtcg
    11881 ttcccgagtc ggccggcgtc atcgtccgca cggccgccga aggtgcctcg gaggaggagc
    11941 ttcgggccga cgtcgagcgc ctcacggcga tctgggaaca gatcaaggcc aaggccgagg
    12001 cagccggcag cggcaagaag ggcaagggca actcgcccgt cctcctgcac ggcgagcccg
    12061 acctcacggt gcgcgtcatc cgggacgtgt tcaacgagga cttcgcccag atgatcgtcg
    12121 ccggcgacga ggcctgggcc cagatcaacg actacgtcgg ctcggtcgct ccggacctcg
    12181 cgtcccgcct gaccaagtgg accggtgagc gcgacatctt cagcaactac cggatcgacg
    12241 agcagttggc caaggccatg gaccgcaagg tctggctgcc ctcgggtggc tcgctcgtca
    12301 tcgaccgcac cgaggcgatg acggtcatcg acgtcaacac cggcaagttc gtcggcgccg
    12361 gtggcaacct cgaagagacg gtcacccgca acaacatcga ggccgccgag gagatcg
//
LOCUS       DAORPW010000063        12246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1088_length_12246_cov_872.920023, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000063 DAORPW010000000
VERSION     DAORPW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12246
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1088_length_12246_cov_872.920023"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLB86_06830"
     CDS             complement(<1..444)
                     /locus_tag="PLB86_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRC64887.1"
                     /translation="MTSPLLRTIRESVIGDDQVLPGPYGPRRVTYADYTASGRALSFI
                     EDFIRDEVLPRYANTHTESSGTGLQTTRLREDARAIIRDAVGGDEDTVVIFTGSGCTG
                     AIDKLVGILGLRIPSQLEDAHHLAQHIPANARPVVFIGPYEHHSNE"
     gene            complement(603..1019)
                     /locus_tag="PLB86_06835"
     CDS             complement(603..1019)
                     /locus_tag="PLB86_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC64888.1"
                     /translation="MVAFISHTSIDCRNAYTLSVWWEGVLDYVPDPDDPNEPGHEECL
                     IRSRDGSHTLLFIEVPDDKVAKNRIHFDLRPSTGTRDEELERLLAHGARVLDDQRNPN
                     GTGWVVLADPEDNEFCILRSQAEVARHQALAAQPTD"
     gene            complement(1239..2831)
                     /locus_tag="PLB86_06840"
     CDS             complement(1239..2831)
                     /locus_tag="PLB86_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC64889.1"
                     /translation="MSRPTSPPSTEHPLGTRRWWALATIALGVSLIIMDATIVSVALP
                     VVIRDLDLTANQAEWMNAIYSLTFAALLLTVGHLGDLRGRRLLFAGGMGLFMVASVFA
                     GSAQSAEILIGARLLQGVGASAILTSTLATLNSIFRGRERAIAFAVYGATIGGMAAVG
                     PLVGGWLATDVSWRWAFWLNIPFGLLVLAGIWRVVPETRDPGAHSHFDLLGAALSALA
                     MGGAVFALIEGATYGWWRQPSGAPSPVPAVAAAAALSTVLFFVREGRARSAGRPPMID
                     VGLLRLPTLRYGLIAAAVVAFGEFGLMFTLPLLLQGALGYSALGAGWLLVSLAIGAFL
                     VSGATPHLTQAIGQRSVVRIGLALEAVAVAGIALTLSLTIPSWAIAAWLFVYGVGVGM
                     ATAQLSSVIMSDVPPAQGGQASGLQSTVRQLGSALGIAVLGTLLVTSLGAGLQERLDA
                     SGMPPAASVQVVEIVRGSAGAAIPSLAADPQGAGAAYLARAAMVDASRRTAGFAAAAL
                     ALGVLATLALPTPRRPDDDPHL"
     gene            2915..3508
                     /locus_tag="PLB86_06845"
     CDS             2915..3508
                     /locus_tag="PLB86_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC64890.1"
                     /translation="MPRIEAPTVAEHNAMRRRQVIEAAADELRDGGVSSFTPAAVAKR
                     AGLARSSMYQYYPSTDALLGAAITTLLQRSRDRMIAATQAAGSPAERVTAYIEAAIAD
                     AQDGHGASPDLASVAMPEVCRDGVRVLHDQLREPLRSALRDAGVPDPDTAALLVQGLV
                     NAAASAINHGVPLERISAATVDLVLRGTGLLAQADAV"
     gene            complement(3523..4815)
                     /locus_tag="PLB86_06850"
     CDS             complement(3523..4815)
                     /locus_tag="PLB86_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HRC64891.1"
                     /translation="MAKHPAPLPLDEQTVPGGAAEASSAAPSRRSFLGVGAALAGGAG
                     AGLVGYGLGNSQTGVAPSAVAAASSEGAAPDAPWAGSVIPFIGAHQAGISTPPQAHAT
                     FVALNLTPGVGQDGLRRLLRQWTNDASRLQRGIPVLGDPQPDLASPPSRLTITVGLGR
                     GALVEAGLADRAPGWLAPLPAFSVDRLEPRWADGDVLAQVCAEDPIILAHAVRALLLS
                     ATGIAAVHWMQRGFRRPVTSTSAMRNLMGQVDGTVQPAAAELEQSVWIGADGPDWLRG
                     GTSLVLRRIRMDLDTWDALDPQAKEQVIGRRMTSGAPLTGTKETDVPDLAATRDGLPV
                     IPEFAHIRHAAVTTAGQKILRRPYNFDDTPAAGSSAEAGLLFAAFQADPVRQFVPMQQ
                     RLAAGDLLNFWTTPIGSAVFAILPGPAEGEILGQALVG"
     gene            complement(4808..5452)
                     /locus_tag="PLB86_06855"
     CDS             complement(4808..5452)
                     /locus_tag="PLB86_06855"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRC64892.1"
                     /translation="MSNHTIRAVTALAALSVALGLGACGTTTATNPVGAAASTAAGTA
                     ASTAPGSAAKATGQAASAQVADAWVKAVDTGMTGAFFVLKNTGSTPIHLLSASSPISP
                     MVQMHETVMGTGGAMAMQEKQGGFTIPAGGSHEFKAGGDHVMFMGVSTAVKSGTTVTF
                     TLAFEDGSTLPVTAEVRTFTGAKETYVPGATTGTNSMPGMTPTPGTSGTTASHG"
     gene            complement(5595..6230)
                     /locus_tag="PLB86_06860"
     CDS             complement(5595..6230)
                     /locus_tag="PLB86_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64893.1"
                     /translation="MTASPTGGGVRVLRATTLAVVTLALAATAHVLAGGDLPGVLALA
                     LAAGPLALGTVLLTRRRLSLAAVFAWLVPAEITLHVYFGAAAPHPLVPAPAGGHVHGG
                     SDAGMPVAGLPGLDPLAQPIGTTPLGHSSLAMLGAHALATVITGLALSHGERVLWLLW
                     ESLRPALALDPVPPATSPAPAALAPVTRRCSSVFLCSPHPRGPPTRRPAVA"
     gene            6263..6808
                     /locus_tag="PLB86_06865"
     CDS             6263..6808
                     /locus_tag="PLB86_06865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRC64894.1"
                     /translation="MDPAPGDSRHDPAVGSAAGSAVGSTVGSAQGPRTFSWDFQVRFI
                     DLNASGHLDNLQILRAVDEARHRLLGVGEPGAAPLLVGLLADAPAHVATLVAAHRVEY
                     RRELWFAAAPLDITLWVCRVGGSSFDVATEVRQSPAEPIAAVAVTTVVLVDTTTRSPW
                     PLSAPVRAALTAYLGPAPTLR"
     gene            6878..8179
                     /locus_tag="PLB86_06870"
     CDS             6878..8179
                     /locus_tag="PLB86_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRC64895.1"
                     /translation="MADIAYPIAERTLPNGLRVIVSQDRTVPSVTVNLWVKVGSRDEV
                     PGRTGFAHLFEHLMFQGSANVASGEHFSALMGVGGRLNATTWFDRTNYFETVPKGALE
                     LALWLEADRQGRLLDALTQENLDNQRDVVKEEKRQRYDNAPYGHAMRDVYATLFPEGH
                     PYHHPTIGSMEDLEAATLDDVHAFFATHYGPNNTVLTLVGDISPEDGFAAVEKYFGPL
                     PPSAVARTEGSMPPLAPLTGPVRLERSEPVPNDRLYLAFRLPADATPEFHAVGLAMDA
                     IGGLSSSRLVEHLVRQERLVTGLTAHPLGFAGGVSLGLISADISAGVDPADVERAICA
                     ELERFAETGPTDIEMESAVAETERAWLSALASHDDRADHISHYAALHDDTAYINTFLA
                     QVRAVTVEEVRAAAARYLQPSSRAVVAYLMAPGPIADEELS"
     gene            8176..9567
                     /locus_tag="PLB86_06875"
     CDS             8176..9567
                     /locus_tag="PLB86_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRC64896.1"
                     /translation="MTGVPQRTRMSAPDALPRPDVLVPQPYELPVPTRFRLANGASGL
                     AYDLPGQYVLSVRLSVPMPLRVEPRDLEGVATIMARTLDEGTARHTSVEFARLLERRG
                     VAIGCGVGDSGLSVELDVAKHHLAYGLDLMHQCLAEPAFPAEEVARQVSTRLAEIEQE
                     RSVASHRAAAEFAATFYSPVGRSSRPVGGTAETVAAITRDAVVAFHAEHVAPAEATVV
                     VAGDLTGLDVVAELDASLGRWGTPDAYRSPGPWQVTPRADDRARIVVVDRPGSVQTEL
                     MIGAPGPDRRHPGGWAPYPLLGFLVGGSPTARIDAVLREDKGYTYGIRSTFRPRRRGG
                     VFITAGSVRSEVTGDALRLLLDVLEHGREGFTQDEVDHAAQFLVLTAPSRYATADTIA
                     DEAASLSFDGLDTGFTSRNLVEIAGMDAERAGAAYREFIDGRWTVVLVGDAASFLADV
                     QALGRGDVTVVPN"
     gene            9578..10084
                     /locus_tag="PLB86_06880"
     CDS             9578..10084
                     /locus_tag="PLB86_06880"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRC64897.1"
                     /translation="MRSALVVDSPIGPLRLESVDGVAVSAIRFDGRPEASDPTSAQAP
                     TAAVLVEAARQLAAYFAGSLRSFDLPLAPHGTDFQRAVWHELALIPYGTTVTYGEVAL
                     RLGLPVSASRAVGAANGANPIPVVLPCHRVIGADGTLTGYAGGLARKQTLLHLEGVAT
                     ERDQLELF"
     gene            complement(10094..10873)
                     /gene="hisN"
                     /locus_tag="PLB86_06885"
     CDS             complement(10094..10873)
                     /gene="hisN"
                     /locus_tag="PLB86_06885"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRC64898.1"
                     /translation="MSYDDDLRLAHVLADAVERVTMARFRAEDLMVETKPDLTPVSDA
                     DRAAEELIRHQLARTRPRDAVVGEEFGTTGHGPRRWIVDPIDGTKNFVRGVPVWATLI
                     ALVDGDEPVVGLVAAPALARRWWAAKGAGAYAGRSLSAARPLHVSGVSALADASLSYA
                     SLGGWSEIDRREDFLDLMDGCWRTRAYGDFWSYMLVAEGAVDIAAEPSLQTYDMAALV
                     PIVTEAGGRFTGLDGRPGCWSGNAVATNGLLHETVLDLIGD"
     gene            complement(10914..11678)
                     /locus_tag="PLB86_06890"
     CDS             complement(10914..11678)
                     /locus_tag="PLB86_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRC64899.1"
                     /translation="MSTPTPEDAADRETASAQGSGLAAHGVSGHPSTPTARLVGALPV
                     IVGFIALAIGVTVTQETYESVQSGRGAAAFDEPVQTWMVAHRAPWLDTVATWFTHLGG
                     KVGMTILATVTVVALARRWRTWSPVLIMLWATAGSLTLTTLGKTLTARARPPAAQAVP
                     PLETSASFPSGHTLNAVLVTSIIGYLVLLHVRSRRVQVLTWCGLALFATLIGLSRIYL
                     GHHWLTDVIAGAAVGGAWAAAVILGHHLYLRLRADP"
     gene            complement(11659..>12246)
                     /gene="rsgA"
                     /locus_tag="PLB86_06895"
     CDS             complement(11659..>12246)
                     /gene="rsgA"
                     /locus_tag="PLB86_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRC64900.1"
                     /translation="AYDAGLEPLLVLTKADLVPADDFIGQYAPLAVRCVITSRRPDRT
                     LIGLEDVRAQLAGRVSVLVGHSGVGKSTLVNALVPGADRSVGRVNDVTGRGRHTSTSA
                     IALRLPNDPGWVIDTPGVRSFGLAHVDVARIVSHFPDLEPGTETCPRGCTHDEPECGL
                     DEFVANGGAGASGPARLDSFRRLLRARTGDVDSDA"
BASE COUNT         1833 a         4236 c         4432 g         1745 t
ORIGIN      
        1 ctcgttggag tggtgctcgt agggcccgat gaagacgacc ggtctggcgt tggccgggat
       61 gtgctgcgcg aggtggtggg cgtcctcgag ttggctgggg atgcgcaggc cgaggatccc
      121 gaccaacttg tcgattgctc cggtgcagcc cgatccggtg aagatgacga ccgtgtcctc
      181 gtcgccgccg acggcgtccc ggatgatggc tcgcgcgtct tcacgcagcc gggtcgtctg
      241 caggccggtg ccggaagact cggtgtgcgt gttggcgtaa cgggggagca cctcgtcgcg
      301 gatgaagtcc tcgatgaacg acagggcgcg gccggaagcg gtgtagtcgg cgtaggtcac
      361 ccgacgcggg ccatagggac ccggcaggac ttggtcgtcg ccgatgacgc tctcgcggat
      421 ggtgcgcagc aaagggctgg tcacggcgac aggatagcgc caggttttgg ctttccgtca
      481 agccataacc ccgcattatg cagccctcta cccggagata gcgctcgcac ggtcatgttt
      541 cagggtctga aagccgtaat gacccccata aatgaccgtg cgagcgctat ctccgtggtg
      601 ggtcagtcgg tgggctgggc ggcgagggct tggtggcggg cgacctcggc ctggctgcgc
      661 aggatgcaga actcgttgtc ctcgggatcg gccaggacga cccaacccgt cccgttcggg
      721 ttccgctgat cgtcgagcac ccgggctccg tgggcgagca ggcgctcaag ctcctcgtcg
      781 cgcgtgccgg tgctgggccg caggtcgaag tggatccggt tcttggcgac cttgtcgtca
      841 ggcacctcga tgaacagcaa ggtgtggctg ccatcgcggg accggatgag gcattcctcg
      901 tgccccggct cgttcggatc atcggggtcc ggcacgtagt ccagcacgcc ttcccaccag
      961 accgacaggg tgtaggcgtt gcggcagtcg atgctcgtgt gcgagatgaa ggcgaccatc
     1021 ccggcagtgt cgcagcctcc cggagcccgc cgcgagggat ttgtggctgc aacgccccct
     1081 gaggcgccca ccccccacaa cggagatagc gctcgcatgg tcatgtgtgg gggctggaaa
     1141 cccctgaacg gacggaaaga tgaccgtgcg agcgctatct ccgggtctga agtcgggtgt
     1201 gaagggggag gaggggtggg ggcaggggag ggagtcggtt agaggtgggg gtcgtcgtcg
     1261 gggcggcgtg gtgtgggcag ggcgagggtg gccaggactc cgagggccag tgcggcagcg
     1321 gcgaacccgg ccgtccgccg gctggcgtcg accatggccg ctcgagcgag gtatgccgca
     1381 ccagcaccct gggggtcggc ggccagggag gggatggccg ccccggccga gccacgcacg
     1441 atctcgacga cctgcacgga ggcggcgggc ggcatacctg aggcgtcgag gcgctcctgc
     1501 agaccggcgc cgagcgacgt gaccaggagc gtgccgagca ccgcgatccc cagggccgac
     1561 ccgagttggc gcaccgtcga ctgcagcccg gacgcctgcc cgccctgcgc cggcggcacg
     1621 tcgctcatga tgactgaact cagctgcgcg gtcgccatgc cgaccccgac gccgtagacg
     1681 aacagccacg cggcgatggc ccaggacggg attgtcaggg acagcgtgag ggcgatgccg
     1741 gcgaccgcga ccgcctccaa ggccaagccg atccgaacga cgcttcgttg tccgatcgcc
     1801 tgggtgaggt gaggggtcgc gccggagacg aggaaggcgc cgatggcgag gctgaccaac
     1861 aaccaccccg ccccgagtgc cgagtagccg agtgcgccct gcagcaacag cggcagggtg
     1921 aacatcagcc cgaactcgcc gaacgcgacg acggccgccg cgatgaggcc gtagcgcagc
     1981 gtcggcaggc gcagcaaccc gacatcgatc atcggcgggc gcccagccga gcgggcccgc
     2041 ccttcacgca cgaagaacag caccgtcgac agcgcggcag cggcggctac ggcgggcacc
     2101 ggtgacgggg cgccggacgg ctggcgccac cagccgtacg tcgcaccttc gatgagggcg
     2161 aagaccgccc cgcccatcgc gagagccgac agcgccgcgc ccaggaggtc gaagtggctg
     2221 tgggcgccgg ggtcgcgggt ctccgggacg acgcgccaga tcccggccag gacgagcaga
     2281 ccgaagggga tgttgagcca gaacgcccag cgccaggaca cgtcggtggc cagccatccc
     2341 ccaacgagcg gtccgacggc agccataccg ccgatggtgg cgccgtagac ggcgaaggcg
     2401 atcgcgcgct cccggccgcg gaagatcgag ttgagggtgg ccagggtgct cgtgaggatc
     2461 gccgacgcgc cgacgccctg cagcaggcgc gcaccgatga ggatctcggc gctctgggcg
     2521 ctgccggcga acaccgaggc gaccatgaac aggcccatgc cgcccgcgaa gagcaggcgt
     2581 cgcccgcgca ggtcgccgag atgccccacc gtgagcagga gagcggcgaa ggtcagggag
     2641 tagatggcgt tcatccactc ggcctggttg gcggtcaggt cgagatcgcg aatgaccacg
     2701 ggcagcgcga cggagacgat tgtcgcgtcc atgatgatga gcgacacgcc gagggcgatg
     2761 gtcgccaagg cccaccaccg gcgggtgccg agcggatgct cggtcgacgg cgggctcgtt
     2821 ggcctgctca cgctcctcac aaaccctagt ttatcgacac ctctgtcgga atcacaacag
     2881 gaacgacact cgcgcaggcc aatacactct gggcatgccg cgcatcgagg cccccaccgt
     2941 cgctgagcac aacgccatgc gtcgtcgcca ggtcatcgag gcggcggccg acgagttgcg
     3001 cgacggcggc gtctccagct tcaccccggc ggccgtcgcc aaacgagcgg gcctggcccg
     3061 cagcagcatg taccagtact acccgtcgac cgatgccctc ctcggggccg ccatcacgac
     3121 cctgctgcag cgcagccggg accggatgat cgcagcgacc caggccgccg ggtcgccggc
     3181 cgagcgggtc acggcataca tcgaagcggc gatcgccgac gctcaggacg ggcacggcgc
     3241 ctccccggat ctcgcgagcg tcgccatgcc cgaggtatgc cgcgacggcg tccgcgtgct
     3301 gcacgaccag ttgcgcgagc cgctgcggtc ggcgctgcgc gacgccgggg tccccgaccc
     3361 cgacaccgcg gccctgctgg tgcaggggct ggtcaacgcc gccgcgtcgg cgatcaatca
     3421 cggcgtcccg cttgagcgga tctccgcagc gaccgtggat ctcgtgctgc gcggaacggg
     3481 cctgctcgcc caggccgacg ccgtctgacc tcgccccccc cgtcagccca ccagcgcctg
     3541 cccaaggatc tcgccctcgg ccggtccggg caggatcgcg aacacggccg atccgatcgg
     3601 ggtcgtccag aagttgagca ggtcgcccgc ggccaggcgt tgctgcatcg gcacgaactg
     3661 cctgaccggg tccgcctgga aggccgcgaa gagcagcccc gcttcggcgc ttgaccccgc
     3721 cgcaggcgtg tcgtcgaagt tgtagggacg gcgcaggatc ttctgcccag cggtggtgac
     3781 ggccgcgtgc ctgatgtggg caaactccgg aatgaccggc agcccgtcgc gcgtggctgc
     3841 caggtcgggg acgtcggtct ctttggtgcc cgtcagcggt gccccactgg tcatccgccg
     3901 accgatgacc tgctccttgg cctgcgggtc gagcgcgtcc cacgtgtcca gatccatccg
     3961 gatccgccgc agcaccagtg aagtgccgcc gcgcagccag tcggggccgt ccgcgccgat
     4021 ccataccgac tgctcaagct cggccgcggc cggctgcacc gtcccgtcga cctggcccat
     4081 gaggttacgc atcgcgctcg ttgaggtcac gggcctgcgg aagccgcgct gcatccagtg
     4141 cacggcggcg atccccgtgg ccgacagcaa cagggcgcgc accgcgtggg caaggatgat
     4201 cgggtcctcg gcgcagacct gggcgagcac gtcgccgtca gcccaccggg gctcaagtcg
     4261 gtcgacggag aacgcgggca gcggcgcgag ccacccgggt gcccggtcgg ccaagcccgc
     4321 ttcgacgagg gctccgcgcc ccaaccccac ggtgatcgtc agccgggagg ggggcgacgc
     4381 gagatcgggc tggggatccc ccagcacggg tatgccgcgc tgcagccggc tcgcgtcgtt
     4441 ggtccactgg cgcagcagcc ggcgcaggcc gtcctggccc acccctggag tgaggttgag
     4501 cgcgacgaag gtcgcgtgcg cctgaggcgg ggtgctgatc ccagcctgat gcgcgccgat
     4561 gaaggggatc accgaccccg cccatggcgc gtcgggagcc gcgccctcgc tggaggctgc
     4621 ggcgacggca gagggagcaa cgccggtctg gctgttcccc aagccgtagc cgacgagccc
     4681 ggctccggcg ccgccggcca aggccgctcc gacgccaagg aacgaccgcc gcgacggcgc
     4741 ggccgaggaa gcctcggccg caccgcccgg caccgtctgc tcgtccaggg gaagcggagc
     4801 cgggtgctta gccatgagaa gccgtcgtcc cagacgtgcc cggggttggt gtcatgccgg
     4861 gcatggagtt cgtgcccgtc gttgcgccgg ggacgtaggt ctccttggcg cctgtgaagg
     4921 tccgcacctc tgccgtcacc ggaagggtgg agccgtcctc gaaggcgagg gtgaaggtca
     4981 ccgtcgtccc cgacttcacc gccgtgctga cgcccatgaa catcacgtgg tcaccgccgg
     5041 ccttgaactc gtgcgagccg ccggccggaa tggtgaagcc gccctgcttc tcctgcatgg
     5101 ccatggcgcc gccggtgccc atgacggtct cgtgcatctg caccatcggg ctgatcgggc
     5161 tgctcgcgga caggaggtgg atcggtgtgg agccggtgtt cttgaggacg aagaacgccc
     5221 cggtcatgcc ggtgtcgacc gccttgaccc aggcgtcggc gacctgcgcg gaggccgcct
     5281 gcccggtcgc tttcgccgcg ctgccgggtg ctgtcgacgc ggccgtgccc gctgcggtgc
     5341 tcgctgcggc gccgacgggg ttggtcgcgg tggtggtgcc gcaggccccc aggcccaggg
     5401 ccacggagag ggcggcaagc gcggtgactg ctcgaatcgt gtgattgctc aacggaattg
     5461 gtccttgtct gacagaggga agggaaggtc cttcaggcgc ggcgggggac gcctcggcgc
     5521 cgaggaaggg caaaggtgga gggtgccgtc gggacggcgg tgaaccaggc gatgggttcg
     5581 gcgctgatca cgcgctacgc gaccgcgggt cggcgcgtcg gaggcccgcg agggtgcggc
     5641 gagcacagga acaccgagga gcaccgccgc gtgactggtg ccagggccgc gggggccggg
     5701 ctcgtcgcgg gcggcacggg gtcgagggcc agggcaggtc tgagtgactc ccacaacagc
     5761 cagaggacgc gctcgccatg gctgagggcc aggccggtga tcacggtggc gagggcatgc
     5821 gcgccgagca tggccaggct tgagtgcccg agcggggtcg tcccgatcgg ctgcgcgagt
     5881 ggatcgaggc cgggaagtcc ggcgaccggc atacctgcgt cggagccgcc gtggacgtga
     5941 ccgccggccg gcgcgggcac cagcgggtgc ggcgcggccg cgccgaagta gacgtggagg
     6001 gtgatctcgg ccggaaccag ccaggcgaac actgcggcga gggagagccg gcgtcgagtc
     6061 agcaggaccg ttccgagcgc caagggaccg gcagcgaggg cgagtgcgag gacgccaggc
     6121 aggtcgcctc cggccagcac gtgagccgtc gctgcgagag ccagcgtgac gacggccagg
     6181 gtggtcgcac gcagcacgcg cacaccgccg cccgtcgggc tcgccgtcat ggccttcagg
     6241 gtaagggcct agggtttcga gcatggaccc ggcccctggt gactcccgcc acgacccagc
     6301 cgtcggctcg gcagccggct ctgcagtcgg ctcgacagtc ggctctgccc agggtccgcg
     6361 gaccttctcc tgggacttcc aggtccggtt catcgacctc aacgccagcg gtcacctgga
     6421 caacctgcag atcctgcggg ccgtcgacga agcccgccac cgcctgctcg gcgtggggga
     6481 gcccggcgcc gcaccgctgc tcgtcggcct gctcgcggac gcgccggcgc acgtcgcgac
     6541 gctcgtcgca gcccaccgcg tggaataccg ccgggagctc tggttcgccg ctgcgccgct
     6601 ggacatcacc ctctgggtat gccgtgtcgg cggctcctcc ttcgacgtcg ccaccgaggt
     6661 tcgccagtct ccggcggaac cgatcgcagc ggtcgccgtc acgaccgtcg tcctcgtcga
     6721 tacgacgacc agatctccct ggcccctgtc ggcccccgtc agggctgcgc tcacggcata
     6781 cctggggccg gccccgaccc tgcgctgacg gcgcccgctt gggcggggcc ccggcaaccg
     6841 gaacacgggg gtgcctttcg cccgataagg tctgcgggtg gccgacatcg cctacccgat
     6901 cgctgagcgg acgttgccca acggcctgcg cgtcatcgtt tcgcaggacc gcacggtgcc
     6961 atccgtgacc gtcaacctct gggtcaaggt cgggtcgcgc gacgaggtgc cggggcgcac
     7021 tggtttcgcc cacctcttcg aacacctgat gtttcagggc tcggcgaatg tcgcctcggg
     7081 agagcacttc tcggcgctga tgggcgttgg tggccgcctc aacgcgacga cttggttcga
     7141 ccggacgaac tacttcgaga cggttcccaa gggggcgttg gaacttgccc tgtggctcga
     7201 ggccgaccgg cagggtcgac tgctcgacgc gctcacccag gagaacctcg acaatcagcg
     7261 cgacgtcgtc aaagaagaga agcgccagcg ctacgacaac gcgccctacg gtcatgcgat
     7321 gcgcgacgtc tacgcgacgc tcttccccga agggcacccc taccaccacc cgacgatcgg
     7381 gtcgatggag gacctcgaag ccgcgacgct cgatgacgtc cacgccttct tcgccacgca
     7441 ttacggcccg aacaacacgg tgctcaccct cgtcggcgac atttctccgg aggacggctt
     7501 cgctgccgtc gagaagtact tcggaccgct gcctccctcc gcggtcgccc gcacagaggg
     7561 gagtatgccg cccctcgcgc cgctgacggg cccagtgcgc ctggagcgct ccgagccggt
     7621 gcccaatgat cggctctacc tcgcgttccg gcttccggcc gacgcgacgc cggagtttca
     7681 cgcggtcggc ctggccatgg atgccatcgg cgggctgtcc tcgtctcgac tggtcgagca
     7741 cctcgtgcgc caggagcggt tggtcactgg tctcaccgcc catccgctgg gcttcgcagg
     7801 gggtgtgtct ctcggcttga tttcggccga catctcggca ggggtcgacc ccgccgacgt
     7861 cgagcgcgcc atctgcgctg agctcgaacg cttcgccgag accggaccga ccgacatcga
     7921 gatggagtcg gcggtggccg agaccgagcg tgcctggctt tcggcgttgg ccagccacga
     7981 cgaccgggcc gaccacatca gccactacgc ggcgctgcat gacgacacgg catacatcaa
     8041 cacgttcctt gcccaggtgc gagcggtgac cgtcgaggag gtgcgcgcag cggctgcgcg
     8101 ctacctgcag ccgtcgtcgc gggccgtggt cgcctacctc atggcaccgg gccctatcgc
     8161 cgacgaggag ttgtcatgac cggggtcccg cagcgcaccc ggatgtccgc cccggacgcg
     8221 ctaccccgcc ccgacgtcct ggtgccgcag ccctacgagc tcccggtgcc gacccggttc
     8281 cggctggcca acggtgcgtc cgggctggcc tatgacctgc ccggacagta cgtcctgtcg
     8341 gtgcgtctgt cggtgcccat gccgttgcgg gtcgagccgc gcgacctcga aggcgtcgcc
     8401 accatcatgg cgcgcaccct cgatgagggg accgcgcggc atacctccgt ggagttcgcg
     8461 cgcctgctcg aacggcgcgg ggtggcgatc gggtgcggcg tcggcgactc cggcctgtcg
     8521 gtcgagctcg acgtcgccaa gcatcacctg gcctacgggc tcgacctcat gcatcagtgc
     8581 ctggccgagc cggccttccc cgcggaggag gtggcgcgcc aggtctccac ccggctggcc
     8641 gagatcgagc aggagcgttc ggtcgcctcg caccgcgcgg cggcggagtt cgcggccacg
     8701 ttctattcgc cggtgggacg ttcgtcgcgc ccggtcggcg gcaccgccga gacggtcgca
     8761 gctatcaccc gcgacgccgt cgtcgccttc cacgccgagc acgtcgcacc tgcggaggcg
     8821 acggtcgttg tggccggcga cctgacgggt ctcgacgtgg tggccgagtt ggacgcgagc
     8881 ctgggtcggt gggggacccc cgacgcctat cgcagccccg gcccctggca ggtcaccccg
     8941 agggccgacg accgcgcccg gatcgtcgtc gtcgatcggc ccggttcggt gcagaccgag
     9001 ctgatgatcg gtgcccccgg cccggaccgg cggcatcccg gcggctgggc cccctacccg
     9061 ttgctcggat tcctcgtcgg cggctcgccg accgctcgga tcgacgcggt cctgcgtgag
     9121 gacaagggct acacctacgg catccgctcg accttccggc cgcgccgccg tggtggcgtg
     9181 ttcatcaccg ccgggagtgt gcgttcggag gtcaccggcg acgctctgcg gctgctgctc
     9241 gacgttctcg aacatggccg cgagggcttc acccaggacg aggttgacca cgccgcccag
     9301 ttcctcgtgc tcaccgcccc gagtcgctac gcgacggccg acacgatcgc cgacgaagcc
     9361 gccagcctgt cctttgacgg gttggacacc gggttcacca gtcgcaatct cgtcgagatc
     9421 gccgggatgg acgcggagcg agcgggcgcg gcataccggg agttcatcga cgggcggtgg
     9481 acggtcgtcc tcgttggcga cgcagcgagt ttcctcgccg acgtgcaggc cctgggacgg
     9541 ggcgacgtga cggtcgtgcc caactaggct gcgcgacatg cgctcggctc tcgtcgtgga
     9601 cagccccatc gggccgttgc ggctcgagag cgtggacggg gtcgccgtga gcgccattcg
     9661 gttcgacggg cgacccgagg cgagcgatcc gacgtcagcg caggcgccga cggcagcggt
     9721 gctcgtcgag gcggccaggc agttggcggc ctacttcgcc gggtcgctgc gctcgttcga
     9781 cctgccgctc gcgccgcacg gcaccgattt ccagcgcgcc gtctggcacg agttggcgtt
     9841 gatcccctac ggcacgaccg tgacctacgg cgaggtggcc ctccggttgg ggctgcccgt
     9901 gtcggcctcg cgcgcggtcg gcgcggctaa cggcgccaac ccgatccccg tggtgctgcc
     9961 gtgccaccgg gtcatcgggg cggacggcac actgaccggg tatgccggtg ggctggctcg
    10021 caagcagacc ctcctgcacc tggagggcgt ggctaccgaa cgggatcagc tcgagttgtt
    10081 ctgaccccgg ccgtcagtcg ccgatgaggt ccagcaccgt ctcgtgcagc agcccattgg
    10141 tggcgacggc gttgccgctc caacacccgg gccgtccatc gagaccggtg aatcggccgc
    10201 cagcctcggt gacgatcggc acgagggcag ccatgtcata ggtctgcagt gacggctccg
    10261 cagcgatgtc gaccgcccct tcggccacca acatgtagga ccagaagtcg ccatacgcgc
    10321 gggtgcgcca gcagccgtcc atgaggtcga ggaagtcctc gcgtcggtcg atctccgacc
    10381 aaccgcccaa tgaggcatac gacagcgacg cgtcggcgag ggcagagacc cctgacacgt
    10441 gcagcggacg ggcggcggac aggctgcgac cggcatacgc cccggctccc ttggccgccc
    10501 accaccggcg ggcgagagcg ggggcggcga ccagcccgac gacgggctcg tcgccgtcga
    10561 ccaaggcgat gagcgtggcc cacacgggga ctccgcggac gaagttcttt gtcccgtcga
    10621 tcgggtcgac gatccagcgg cgcgggccat gcccggtcgt gccgaactcc tcgcccacca
    10681 cggcgtcgcg cgggcgggtc cgggccagtt ggtggcggat gagttcctcc gccgcgcggt
    10741 cggcatcgct caccggggtg aggtccggct tggtctcgac catcaggtcc tcggcgcgga
    10801 accgggccat ggtcacccgc tcgacggcat cggcaaggac gtgggccagg cggagatcgt
    10861 cgtcgtaact cacggtcaca ggctagaggt atgccgcccg ctcaccgtgg tggctagggg
    10921 tcggcgcgca gtcggagata gaggtgatgg ccgaggatga cggcggccgc ccaggcgccc
    10981 ccgacagcgg cgccggcgat gacgtcggtc agccaatggt ggccgaggta gatccgggac
    11041 aggccgatga gggtcgcgaa cagggccaac ccgcaccagg tgagcacctg cacccgccgg
    11101 ctgcgcacat ggagcaggac gaggtatccg atgatgctgg tcacgaggac ggcgttgagc
    11161 gtgtgcccac tcgggaacga cgcggatgtc tcgagcggtg gcaccgcctg cgccgccggg
    11221 ggacgcgcgc gggcggtcaa cgtcttgccg agggtcgtca acgtcagcga ccccgccgtc
    11281 gcccacagca tgatgagcac cggtgaccag gtgcgccagc gacgggccag ggccaccacc
    11341 gtgacggtgg cgaggatcgt catcccgacc ttgccgccca ggtgggtgaa ccaggtcgcc
    11401 accgtgtcca gccaaggcgc gcgatgggcg accatccacg tctgcacggg ctcgtcaaac
    11461 gcggcggccc cacgcccgga ctgaaccgac tcgtaggtct cttgggtcac cgtcacgccg
    11521 atggccaacg cgatgaaccc cacgatgacg ggcagcgccc cgaccaagcg cgccgttggt
    11581 gtgctcgggt ggccggagac cccgtgggcc gcgagtcccg atccctgggc gctcgccgtc
    11641 tcgcggtccg cggcgtcctc aggcgtcgga gtcgacatcg ccggtgcggg cgcggagcag
    11701 tcggcggaac gagtcgaggc gagccgggcc gctggcgccc gcgccgccgt tggcaacgaa
    11761 ttcgtccagg ccgcactcgg gttcgtcgtg ggtgcagcct cgcgggcagg tctcggtgcc
    11821 cggctcaagg tcggggaagt gcgacacgat ccgggcgacg tcgacgtgcg ccaacccgaa
    11881 ggagcgcacc cctggcgtgt cgatcaccca ccccggatcg ttcggcagcc gcagggcgat
    11941 ggccgacgtg gaggtatgcc gtccgcgccc cgtgacgtcg ttgacccggc ccacgctgcg
    12001 gtcggctcca gggacgagag cgttgacgag ggtggacttg ccgacccccg aatgcccgac
    12061 gagcaccgac acccggccgg cgagttgggc ccgcacatct tcgaggccga tgagggtgcg
    12121 atcgggtcgg cgggaggtga tgacgcagcg cacggccagc ggggcgtatt gcccgatgaa
    12181 gtcgtcggcc ggcaccaggt cggccttcgt gaggacgagc agcggctcca gcccggcgtc
    12241 gtaggc
//
LOCUS       DAORPW010000064        12225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1095_length_12225_cov_800.346836, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000064 DAORPW010000000
VERSION     DAORPW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1095_length_12225_cov_800.346836"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..1508)
                     /locus_tag="PLB86_06900"
     CDS             complement(348..1508)
                     /locus_tag="PLB86_06900"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HRC64901.1"
                     /translation="MGAPRQRPAVTLREPSSGTTERTPQRLGSLDVARGAFVVLSLCA
                     VAVLEPRPSWLTHAAWFGITPIDVVFPTFVTLSGCGLAFAYGRSVAARPTVRRFIVLL
                     LAGLTYNALTGPDRIDLATMWVTGPLQTYALLVLLIALLHLVLRTYAWWALLTIALAG
                     VWDILLYRFAQTCPGGVLLPECNPSAIIDSAVFGVHMYDHGATGHDPVGLVAFIGTFV
                     TAAAGVTAGHLIRAFSPRPRLLAALLAGWSCVVGGAGLALAVWVPAFKRLWTPSFGLQ
                     TAAVGLALLCLCYVSVDLPRGPLLRKATSVAIWPLVALGRNALLVYFGSHVIIVLLIR
                     STGSVKGQTMADDLANSLAINGSSRLGLVVANLLAWWLLAAVLHRSRVYIHA"
     gene            complement(1522..3741)
                     /locus_tag="PLB86_06905"
     CDS             complement(1522..3741)
                     /locus_tag="PLB86_06905"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRC64902.1"
                     /translation="MTSEIVWTQIDEAPALATYSFLPIVQAFTRGTGVDVVTSDISLS
                     GRILSAFPERLAPEQQVPDNLAALGALTLRPEANIIKLPNISASVPQLKAAIKELQDK
                     GYAVPDYPEAPATDEEKAVHAAYAKVLGSAVNPVLREGNSDRRAPLSVKNFAKKHPHR
                     LGAWDPATKARVETMTAGDFYGNEQSVVVDGGGDLRIEHVGADGSVTVLKDKVATLPG
                     EIVDGTFMSVAALRDFYAAQIDAAKADGLLLSLHLKATMMKVSDPVMFGHAVSVFYAD
                     VFAKYADTLAALGVDPNMGMGDLEAKISGLPATERAAIAADIAAVYAARPAIAMVNSD
                     KGITNLHVSSDTIVDASMPVVIRDSGQMWNWDGKLQATLAMVPDRSYGLMYAAVFEDC
                     RVNGALDPATMGSVPNVGLMAQKAEEYGSHPTTFIVPAAGTVRVLGPDGALLMSHEVG
                     AGDIWRMSRVRDIPIQDWVKLAVTRARITGAPACFYLDSARAHDRNVIAKVERYLGDH
                     DLTGVEYRILAPVEAIQWCLARIREGKDTISVTGNVLRDYLTDLFPILELGTSAKMLS
                     VVPLLAGGGLFETGAGGSAPKHVQQFVAEDYLRWDSLGEFSALGASLEHLAQTQGNAK
                     AQVLAVALDKAIATFLDENKSPARKIGQIDNRGSHFYLALFWAEELAAQSDDAELAAR
                     FAPVAAALRSNESTINEELLAVQGKPVDMGGYYRPDTAKTGAAMRPSATLNAVIDAL"
     gene            3947..4723
                     /gene="aqpZ"
                     /locus_tag="PLB86_06910"
     CDS             3947..4723
                     /gene="aqpZ"
                     /locus_tag="PLB86_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HRC64903.1"
                     /translation="MQPALSKRLGAELLGTFWLVFGGCGSAIFAAKFYAPAGVADAQG
                     TEVGIGHLGVSLAFGLTVLTMAYAVGHVSGGHFNPAVTIGLWAARRFDNVRDLFAYIG
                     AQVVGGLLAGACLWGLAKSAKGSGSMVGNMAANGYGDNSPGGYSMWAVLLAEVLLTAF
                     FLFVILGVTTKHAPAGFGPLAIGLTLTLIHLISIPISNTSVNPARSTAVAFFNGAGAP
                     GQLWLFWVAPIVGALIAGWSYEAITGIDASAEDVSGSLAK"
     gene            4788..5423
                     /locus_tag="PLB86_06915"
     CDS             4788..5423
                     /locus_tag="PLB86_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRC64904.1"
                     /translation="MFVVTADQVDSRREADRVPEALTLLAGVRPRAANPFERTAGDEI
                     QGVTDDPADLLDIVTSLIRADRWRIGIGIGPVDLPLPASPRAGRGPAFIAARAAVSAA
                     HPSTGQLAVRFGATPELDPHLRAVRERHTRYAESALLLLARLLRGRTAEGWEVTDLLA
                     DGLTQKEAAARLGVSPSAVSQRVRRSGWSEQVGAVELVQHLLSVADGRTDP"
     gene            5420..5821
                     /locus_tag="PLB86_06920"
     CDS             5420..5821
                     /locus_tag="PLB86_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64905.1"
                     /translation="MTVTQALLGGAILLAAVLAIFGGSPLTGWVFAHVERRSGATPGQ
                     TVAGISAAAVVLRGGQTIGLLERTAVFLGILAGWPEALVGVIALKGLGRFAELRGTTD
                     GAAERFIIGSFTSLLWAAVLGGLARLALEHY"
     gene            complement(5836..6594)
                     /locus_tag="PLB86_06925"
     CDS             complement(5836..6594)
                     /locus_tag="PLB86_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRC64906.1"
                     /translation="MLAVANQKGGVAKTTTVASLGAAFAEMGKRVLLIDLDAQACLTF
                     SLGVDPDTVAVSINEVLIGQAGIREAIVECADGVHLVPSVIDLAGAEAQLLGRPGREF
                     VLRTILEDVAEDYDIVLLDCSPSLGVLTLAALTAAQGLLIPMPCEMLSHRGVGQLLDT
                     VRDVTRLLNPKLEVIGVLPTLFDGRSAHSQAVLADVGQRYGLPVIEPPIPKTIRFAEA
                     PAVGRSILTTSKSSKGARAYRAAAKSLAGRIWGA"
     gene            complement(6614..7600)
                     /locus_tag="PLB86_06930"
     CDS             complement(6614..7600)
                     /locus_tag="PLB86_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRC64907.1"
                     /translation="MSDAQPRAGKATQGEFAGMPTPLYAASPSRLLAWLDCPRRYRFQ
                     YLDKPRPAVRPPRAHTSIGVAAHNALRDWWDQSDRTPEAGARLVRESWVDLGFRDAAQ
                     SATWRERTQGEVTAYLSGVDPRRTPVGIERTAAFKTDALAFFGRIDRLDDRDGELVVV
                     DYKTGRRPLSEEDARTSLPLALYAAAVWRMFRRRCVRVELHHVPTGKVAAYEHSDESL
                     VRKVREAESIAADLRRVDAVYAVDGTDAACFPPRRSPLCQWCDYRAHCREGQQAGPEK
                     SSWAALEESTDDRVTDEAHFGGTGEVDLVVGVREPTGPASDGPQRPDGPIGG"
     gene            complement(7627..8487)
                     /locus_tag="PLB86_06935"
     CDS             complement(7627..8487)
                     /locus_tag="PLB86_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRC64908.1"
                     /translation="MRIDLHAHTTCSDGTQSPTELVAAARAAALDVVAITDHDTLTGW
                     AEAGAAAIEHGIALVPGVEISCERGGRSVHLLGYLVDPAYDPLAAELAKARDSRVTRL
                     ERMVALLAADGIPLTYAAVLAQVPPGATPGRPHIADALVAAGVVATRDEAFERWLHDD
                     SPYYVHHYAPDPLRAVELVRAAGGVAVLAHPFAATRGAVILADEVISHLADAGLGGIE
                     ADHPDHDEAARARARALATRLGLFVTGSSDYHGSGKTTRLGDNLTDPAVFAALERAGS
                     GSAIIRPASH"
     gene            8530..9456
                     /locus_tag="PLB86_06940"
     CDS             8530..9456
                     /locus_tag="PLB86_06940"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRC64909.1"
                     /translation="MAKATGVPAYTLQQLAAFVAVADTGTIAAAAVRLQLSPSAVRAS
                     LGDLERALGAQLCVRHRAKGVELTSGGKAVLAKARVVLHEANDLELDVRGEPGQVTGV
                     LHVGCYTTLGPTVLPPLLDAFRRRYPAARIELREDTLDRLRTAVDARDVDVTIAYDID
                     LPRHWSTAAWLTSAPRVHVAAGHRLAEQDGVRLLDLAEDPMILLDAPPSREHVLDMCR
                     GAGFEPRVALRTSNFETARALVGRGLGWALLVQRTATDVSYEGRQVIARDVLEPLLPS
                     VPIVVAWPRDARLSRAAQAFVQLVARHAAQAG"
     gene            9614..11245
                     /locus_tag="PLB86_06945"
     CDS             9614..11245
                     /locus_tag="PLB86_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRC64910.1"
                     /translation="MTSSLDPNVPAIVLGCGPVGQTAALCLAAWGVPVVMLDARPERD
                     PIGSKALCQQRDVLDVWDHVGAGRQIAAEGVTWTRAVTLYQGKELFTQGFVDRGRSPF
                     PPFVNIGQNRTEELLDEQIRANPLITEKWGHTVESVEQDADSITVVCRVGTHGATETV
                     RITGAYVIAAPGNKGHLVRDMVGMTFEGTTFDDQFLICDIRTTLPGWELERRFYFDPE
                     WNPGRQVLIHPCPDSTYRIDWQVPPNYDLASEEASGALDTRIRQIVGDVDYEIVWKSA
                     YRFHARQVDQMVAGRLLMAGDGAHLVAPFGARGLNSGVPDAENAAWKVAFVMHGWADP
                     SLISTYHDERHAAAAENLDVTAATMRFLVPQNEQEWASRREILEGAAAHPDDLDLRAQ
                     VNSGRMAEPFWYVDSPLTTPSADHPFAGRPPKGEDPVAAPGVIAPDVEVTLAGGDRVR
                     IRALARRGLLVLVGADLDAEAVAEAMAGLRCQTFVLPLAEVDPDGSVAQALGAHPGEA
                     WVFRPDAHIAAVATSTTDAASAAYRAVGATAPATV"
     gene            11343..>12225
                     /locus_tag="PLB86_06950"
     CDS             11343..>12225
                     /locus_tag="PLB86_06950"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRC64911.1"
                     /translation="MAYYRSAGNIPHKRHVQHRDPEGKLYYEELMGEEGFSSDSSLLY
                     HRFIPAAIREVRPWTLPDQTLRANDPMIPRHFKTQSLPGDGAGIDAVNGRRLLLGNGD
                     VRLSYVVAKDPSPYYRNAIGDECLYVHGGTARVETVFGDLDVWEGDYVIVPRATTHRI
                     VPTGDAPVRIYAIEVNSHITPPKRYLSRFGQLLEHSPYCERDLRVPTDPYVVEETDIP
                     VHIKHRGPGANGISGTIHVQPHHPFDVVGWDGCLWPYAFNISDYEPLTGRVHQPPPTH
                     QVFEAHNVIMCNFVPRKV"
BASE COUNT         1967 a         4270 c         4162 g         1826 t
ORIGIN      
        1 agcgatgccg cctggctggg tcctctgatc gcgcctgagg acggttcgga aggtccgtcc
       61 cggcaaagac gaccatgcga gcgctatcac cgggccaaac cccggggtca gcaccgcaaa
      121 gatgaccatg cgagcgctat caccgggcca aaccccggga tcaccgccgc aaagatgacc
      181 gtgcgagcgc tatctccggg atgggagagg cgggacgtct cggtgggcat gagccggaaa
      241 cccagacgtc gggctcaggg gcgagtgcct acccgcgcaa gcgcgcagtc cgcgacaaca
      301 agacccctga cgttgcagga gaaccgcgca gagtcatcac ccgaagctca cgcgtgaatg
      361 tatacgcgcg aacggtgcag caccgctgct agcagccacc aggcaagtag attcgccacc
      421 accagcccca atcgagagga tccattgatc gccaagctgt tggctagatc gtcggccatt
      481 gtctggccct tgaccgaccc cgtggagcga ataaggagca caatgatgac atgtgagccg
      541 aaatagacca agagtgcatt gcggccaagt gcaaccagcg gccatatcgc cactgaggta
      601 gccttgcgaa gtagaggccc tcggggtaag tcaacgctga catagcacaa acacagaagc
      661 gccaggccga ccgcggccgt ttgcagccca aagctcgggg tccagagtcg tttgaaggct
      721 gggacccaaa cggccagcgc gaggccagca ccgcccacga cgcaggacca gccggcgagg
      781 agggcagcga gcagccgagg ccggggagag aaggccctga tgagatggcc tgccgtgacc
      841 cccgccgctg ccgtcacaaa ggtgccgatg aaagccacga gacccacagg atcgtgccct
      901 gtcgctccat ggtcgtacat atggaccccg aacaccgctg agtcgatgat cgcagatggg
      961 ttgcactccg gcagcagaac tccccccggg cacgtttggg caaatcgata caacagaata
     1021 tcccagaccc cggctagggc gatcgtcaaa agagcccacc acgcataggt tcggagaacc
     1081 aggtgcagga gcgcaatgag cagcacgagc aatgcatagg tctgcagcgg tcccgtgacc
     1141 cacatcgtcg ccaaatcgat ccgatcgggg ccggtcaagg cattataggt gagacccgcg
     1201 agcaagagta cgatgaaacg ccgaactgtc gggcgggcag ccacggacct gccatatgca
     1261 aacgccaacc cacacccaga cagcgtgaca aaagtcggga agaccacatc aataggggtg
     1321 atcccgaacc aggcggcatg ggtgagccag gagggacgtg gctcaagcac ggcgactgcg
     1381 cagagtgaga gcaccacgaa ggcgccccgt gccacatcca gagaccctag acgctgcgga
     1441 gttcgttcag tggtccctga cgacggttca cgaagtgtca ccgcgggtcg ctgacgaggg
     1501 gcacccatcg agggtcccgg cctagagagc gtcgatgacc gcgttcagcg ttgcgctggg
     1561 gcgcatcgct gcgccggtct tggcggtgtc ggggcggtag tagccaccca tgtcgaccgg
     1621 cttgccctgc acggcgagca actcctcgtt gatcgtcgac tcgttcgacc gcagcgcggc
     1681 cgccaccgga gcgaaccgcg ccgccaactc ggcgtcatcc gactgcgccg ccaactcctc
     1741 ggcccagaac agcgcgaggt agaagtgcga gccgcggttg tcgatctgac cgatcttgcg
     1801 ggcgggcgac ttgttctcgt cgaggaacgt cgcgatcgcc ttgtcgagcg cgaccgcaag
     1861 cacctgggcc ttggcgttgc cctgggtctg ggccaggtgc tcaagcgagg cacccaacgc
     1921 cgagaactca ccgagcgagt cccaccgcag gtagtcctcg gcaacgaact gctgcacgtg
     1981 cttgggggcc gacccaccgg caccggtctc gaagagccca ccaccggcga gcagcggcac
     2041 gaccgagagc atcttggccg aggtccccaa ctcaaggatc gggaagaggt cggtgaggta
     2101 gtcacgcagg acgttgccgg tcaccgagat ggtgtccttg ccctcgcgga tccgcgcgag
     2161 gcaccactgg atcgcctcga cgggagccag gatgcgatat tccacgcccg tcaggtcgtg
     2221 gtcacccaag taacgctcga ccttggcgat gacgttgcgg tcgtgcgccc gcgcggagtc
     2281 gaggtagaag cacgccgggg cgccagtgat ccgagcgcgc gtcaccgcga gcttgaccca
     2341 gtcctggatc gggatgtccc gcacgcgcga catccgccag atgtccccgg cgccgacctc
     2401 gtgcgacatg agcagggcgc catcaggccc gagcacgcgc acggtcccgg cggcaggcac
     2461 gatgaacgtc gtcgggtgcg agccgtactc ctcggccttc tgggccatca gcccgacgtt
     2521 gggcaccgag cccatggtcg ccgggtcgag ggcgccgttg acgcggcagt cctcgaacac
     2581 cgcggcatac atgaggccgt aggagcggtc gggcaccatg gcgagggtcg cctggagctt
     2641 gccgtcccag ttccacatct ggccggagtc gcggatgacg accggcatcg acgcgtcgac
     2701 gatcgtgtcg ctggagacgt gcaggttggt gatgcccttg tcggagttga ccatcgcgat
     2761 ggccggccgc gcggcataca ccgcagcgat gtcggcggcg atcgccgcgc gctccgtcgc
     2821 cggcagcccg gaaatcttgg cctccaggtc gcccatgccc atgttcgggt cgaccccgag
     2881 cgcggccaac gtgtcggcgt acttcgcgaa gacatcggca tagaacaccg acaccgcgtg
     2941 accgaacatc accgggtcgg agaccttcat catcgtcgcc ttgaggtgca gcgacagcag
     3001 cagcccgtcg gccttcgccg catcgatctg cgcggcatag aagtcccgca gcgccgccac
     3061 actcatgaag gtgccgtcga cgatctcgcc gggcagcgtg gccaccttgt ccttgaggac
     3121 ggtcaccgag ccatcagcgc cgacgtgctc gatccgcagg tcgccaccgc cgtcaacaac
     3181 cacggactgc tcgttgccgt agaagtcacc ggcagtcatc gtctcgacgc gagccttggt
     3241 cgccgggtcc cacgcgccga ggcggtgcgg gtgcttcttg gcgaagttct tcaccgacaa
     3301 gggggcgcgg cggtcggagt ttccttcgcg cagaacgggg ttcaccgcgg agccaagaac
     3361 cttggcgtat gccgcgtgca ccgccttctc ctcgtcggtc gccggcgcct cgggatagtc
     3421 ggggacggca taccccttgt cctgcaactc cttgatcgcg gccttgagct gcggcacgga
     3481 ggcggagatg ttggggagct tgatgatgtt ggcctcgggg cgcagcgtca gcgcgcccag
     3541 cgcggcgagg ttgtcgggca cctgctgctc cggcgcgagc cgctcgggga aggccgacag
     3601 gatgcgcccg gagagcgaga tgtcgctcgt cacgacgtcg accccggtcc ccctggtgaa
     3661 cgcctggacg atcggcagga aggagtaggt cgccagggcc ggcgcctcgt cgatctgggt
     3721 ccacacaatt tctgaggtca tcgcatctcc gtcgattcga agctggccgg acaggggtcg
     3781 cgacccagga tagcttgacg tcaagatacc tcggtatgcc gcgctcggcc cgtcctctcg
     3841 tcgggttgat cacaccacct cccagccggg cgggtggcga gccggtagcg tagacgccga
     3901 cgaccgcggc aaacgcccga tcggagaacg acaagggaga ccttccatgc agcctgcact
     3961 cagcaagcgg ctcggcgccg aactactcgg cacgttctgg ctcgtattcg gcgggtgtgg
     4021 cagcgccatc ttcgccgcca agttctacgc ccccgccggg gtggccgacg cacagggcac
     4081 cgaggtcggc atcggccatc tcggcgtctc gctcgccttc gggctcaccg tgctcacgat
     4141 ggcgtatgcc gtgggccacg tctccggagg gcacttcaac ccggcggtga cgatcggcct
     4201 gtgggcggcc cgccgcttcg acaacgtccg cgacctgttc gcctacatcg gcgcccaggt
     4261 cgtcggcggc ctgctggccg gagcgtgcct ctggggattg gccaagagcg ccaagggctc
     4321 cggaagcatg gtcggcaaca tggccgcgaa cggctacggc gacaactcgc ccggcggcta
     4381 ctcgatgtgg gcggtgctgc tcgccgaggt cctgctcacg gccttcttcc tgttcgtcat
     4441 cctcggggtg accaccaagc acgcgccggc cggcttcggg ccgctggcca tcggtctcac
     4501 cctgacgctc atccacctca tctcgatccc gatcagcaac acgtcggtca acccggcccg
     4561 ctcgacggcg gtcgccttct tcaacggagc cggcgccccc gggcagttgt ggctgttctg
     4621 ggtggccccg atcgtcggtg cgctcatcgc gggctggagc tacgaggcca tcaccggcat
     4681 cgacgccagc gccgaggacg tgtccgggag cctcgccaag taaggcaaga ggcttcacac
     4741 cagcgagtga agttgttatc cttcatttat cggaatgaag gatggacatg ttcgtcgtca
     4801 cagcagacca ggtggacagt cgccgcgagg ccgaccgcgt ccctgaggcg ctgaccctgc
     4861 tcgcgggggt acgcccgcga gcagccaacc ctttcgagcg cacggccggc gacgagatcc
     4921 agggagtcac cgatgatccc gccgacctgc tcgacatcgt cacgagcctg atccgtgccg
     4981 atcgttggcg catcggcatc ggcatcggtc cggtcgacct gcccctgcct gcgtcgccgc
     5041 gagccggccg agggcccgcc tttatcgctg cccgagccgc ggtttcggcg gcccacccct
     5101 cgaccggcca actcgccgtc cggttcggtg ccacgccgga gctggacccc cacctacggg
     5161 cggtgcgcga gcggcatacc cgatatgccg agtcagccct cttgctcctc gcgcgcctgc
     5221 tgcgcgggcg gacggccgaa ggctgggagg tgaccgacct cttggccgac gggttgaccc
     5281 agaaggaggc ggcggcccgc ttgggggtca gcccgtcagc ggtcagccag cgggtccggc
     5341 gctccgggtg gagcgagcag gtcggcgccg tcgagctcgt ccagcacctg ctgtcggtgg
     5401 cagacggtag aactgacccg tgaccgtgac ccaagcgctg ctcggcggcg ccatcctgct
     5461 ggctgccgtg ctggcgatct tcggcggcag cccgctcacc ggctgggtct ttgcccatgt
     5521 cgagcgccgc tccggtgcga cccctgggca gacggtggct ggcatctccg ccgccgcggt
     5581 cgtgttgcgc ggtgggcaga cgatcggcct gctcgagcgc acggccgtct tcctcgggat
     5641 cctcgccggc tggcccgagg ccctcgtggg cgtcatcgcc ctcaaaggct tgggccgatt
     5701 cgccgagttg cgcggcacga ccgacggggc tgccgagcgg ttcatcatcg gcagcttcac
     5761 cagcctgctc tgggctgccg ttctgggcgg gctggcccgg ctcgctctcg agcactactg
     5821 acgccgcgct gtgactcagg cgccccagat tcggccagca agggacttcg cggcagcccg
     5881 gtaggcgcga gcgcccttgg acgacttgga ggtcgtgagg atcgagcgcc ccacggccgg
     5941 ggcctcggcg aaccggatcg tcttggggat cggcggctcg atgaccggca gaccgtagcg
     6001 ctgcccgaca tcggcgagca ccgcctggga gtgcgccgag cgcccatcga acaacgtcgg
     6061 caggaccccg atgacctcga gcttggggtt gagcagccgg gtgacgtcac gcaccgtgtc
     6121 gagcaattgg ccgacccctc ggtggctgag catctcgcac ggcatcggga tgagcaggcc
     6181 ctgggccgcc gtcaacgcgg cgagggtgag cacccccagg ctcggcgagc agtccaacag
     6241 gacgatgtcg tagtcctccg ccacatcttc caggatcgtg cgcaacacga actcccgccc
     6301 gggacgaccg agcagttggg cctctgcccc ggccaggtcg atgaccgacg gcacgaggtg
     6361 gacgccgtcg gcgcactcga cgatggcctc gcggatgcct gcctgaccga tgagcacctc
     6421 gttgatggag acggccacgg tgtcggggtc caccccgagg gagaaggtga ggcaggcctg
     6481 cgcgtcaagg tcgatgagca acacccgctt gcccatctcc gcgaacgccg cacccaggct
     6541 cgcgacggtc gtcgtcttgg ccacgccacc cttctggttg gcgacggcga gcacgaacgg
     6601 cccggtgcgg gcctcaaccg ccgatcggcc cgtccgggcg ctgcggcccg tccgacgctg
     6661 gacctgtggg ctcccggacg ccgacgacga ggtcgacctc ccctgtgccg ccgaagtgcg
     6721 cttcgtcggt gacgcggtca tccgtgctct cctccagtgc ggcccaggac gacttctcgg
     6781 gaccggcctg ctgcccttcc cgacaatggg cacggtagtc gcaccactga cacaacggcg
     6841 agcgccgtgg cgggaagcac gccgcatcgg tgccgtcgac ggcatacacg gcgtcgaccc
     6901 gtcgcaggtc tgcagcgatc gattcggctt cgcgcacctt gcgcacgagg gactcgtccg
     6961 agtgctcgta ggccgcgacc ttccccgtcg gcacgtggtg caactcgacc cggacgcagc
     7021 ggcggcggaa catccgccag acggccgcgg catacaacgc gagcgggagc gacgtgcgcg
     7081 cgtcctcctc ggagagcggg cgtcggccgg tcttgtagtc gacgacgacc aactcgccgt
     7141 cgcggtcatc gagccggtcg atccgaccaa agaacgccag cgcgtcggtc ttgaaggcgg
     7201 ccgtgcgctc gatccccacc ggggtgcggc gcgggtcaac ccccgagagg tatgccgtga
     7261 cctcgccctg agtgcgttcc cgccaggtcg cgctctgggc ggcgtcacgg aagccgaggt
     7321 cgacccagct ctcgcgaacc agccgggcgc cggcctcggg tgtccggtcg gactggtccc
     7381 accagtcgcg cagggcgttg tgggccgcca cgccgatcga ggtgtgggcc cgcggcgggc
     7441 ggaccgcggg gcggggcttg tcgaggtatt ggaagcggta gcgccgcggg cagtccagcc
     7501 aggcgagcag gcgggacggg ctggcggcat acagcggggt gggcatgccg gcgaactcgc
     7561 cctgggtggc cttccctgcc cgtggttgcg cgtccgacac gctggccacg ctagccggtc
     7621 ggggtgtcag tggcttgccg ggcggatgat cgccgagccg gacccggcgc gttcgagcgc
     7681 ggcgaagacg gccggatcgg tgaggttgtc accgagccgg gtggtcttgc cggagccgtg
     7741 gtagtcgctc gatccggtga cgaacagccc caaccgggtt gcgagcgcgc gggcgcgggc
     7801 ccgcgccgcc tcgtcgtggt cggggtggtc ggcctcgatg ccaccgagcc cagcgtcagc
     7861 caagtgcgag atcacctcgt ccgcgaggat gacggcgccc cgcgtcgccg cgaaggggtg
     7921 agccagcacc gcgactcccc cggcggcccg gacgagctcg acggcccgca gcgggtcggg
     7981 cgcgtagtgg tggacgtaat agggactgtc gtcgtgcagc caccgctcga acgcctcgtc
     8041 ccgtgtcgcg acgacccccg cggccacgag ggcgtcggcg atgtgaggac gccccggggt
     8101 ggcgcccggc ggcacttggg cgagcaccgc ggcatacgtc aggggtatac cgtccgcagc
     8161 caacagcgcg accatgcgtt ccagccgggt gacgcgggag tcgcgcgcct tggcgagctc
     8221 ggccgcgagc gggtcgtatg ccgggtcgac gaggtagccg aggaggtgga ccgaccggcc
     8281 tccacgctcg caggagatct ccacgccggg gacgagcgcg atgccgtgct cgatcgcggc
     8341 ggctcccgcc tcggcccagc ccgtcagcgt gtcgtggtcg gtgatggcca cgacgtccag
     8401 ggcggcggcg cgggcagccg cgacgagctc ggtcggtgac tgggtcccgt ccgaacacgt
     8461 cgtgtgggcg tgcaggtcga tgcgcattga cgcgagccta ggggcgggag cagtcgctag
     8521 agtgccgcca tggccaaggc caccggcgtg ccggcataca ccctgcagca actggccgcg
     8581 ttcgtcgccg tcgccgacac ggggaccatt gccgcggcgg ccgttcgact gcagttgtcc
     8641 ccctctgccg tccgggcctc gctcggcgac ctggagcggg ccctcggagc gcaactgtgt
     8701 gtgcgccatc gggccaaggg cgtcgagctg acctcgggcg gaaaggcggt cctcgccaag
     8761 gcgcgggtcg tgctgcacga ggccaacgat ctggagctgg acgtccgcgg tgagccgggc
     8821 caggtcaccg gagtgctcca tgtcgggtgc tatacgacgc tcgggccgac cgtcctgccg
     8881 ccgctgctcg acgccttccg gcggcgctac cccgcggccc ggatcgagtt gcgcgaggac
     8941 accctcgacc gactgcgcac cgcggtggac gcgcgtgatg tcgacgtgac gatcgcctac
     9001 gacatcgacc tgccccggca ttggagcacc gccgcctggc tgaccagtgc cccgcgggtg
     9061 catgtcgccg ctgggcatcg cctagcggag caagacggtg tgcggctgct cgacctcgcc
     9121 gaggacccga tgatcctgct cgatgcgccg ccgagccgtg agcacgtgct ggacatgtgc
     9181 cgcggcgccg gattcgagcc tcgggtggcg ctgcggacga gcaacttcga gacggcccgg
     9241 gcgttggtcg ggcgggggct gggctgggcg ctgctcgtgc agcggacggc gacggacgtg
     9301 agctacgaag gccgacaggt gatcgccagg gacgtcctcg agccgctgct gccgtcggtg
     9361 ccgatcgtcg tcgcgtggcc ccgcgatgcc cggctgtcgc gggcggcgca ggcgttcgtg
     9421 caactggtcg cccggcacgc ggcgcaggcc ggctaagggg ggtcgtcggg tccgcggggt
     9481 cggtggctgg ccgggcgcgc gggcataacc gttccagaac tgcggaacaa cccacataac
     9541 ttgaactttt cagcgcttgt tgaacgcctc accatgagtg ccagccgagc ctcggtgact
     9601 tctggaggac accatgacca gttcccttga cccgaacgtc cccgcgatcg tcctcggttg
     9661 cggccccgtc ggccagaccg ccgcgttgtg cctggccgcc tggggggtgc ccgtcgtgat
     9721 gctcgacgct cgcccggagc gcgatccgat cgggtccaaa gccctgtgcc agcagcgcga
     9781 cgtgctcgac gtgtgggacc acgtcggtgc cggccgtcag atcgccgccg agggcgtcac
     9841 ctggacccgg gccgtcacgc tctaccaggg caaggagctc ttcacccaag gcttcgtcga
     9901 ccgcggccgc tcacccttcc cgccgttcgt caacatcggc cagaaccgca ccgaggagtt
     9961 gctcgacgag cagatccgcg ccaaccccct catcaccgag aagtggggtc acaccgtcga
    10021 gtccgtcgag caggacgccg acagcatcac cgtggtatgc cgcgtcggca cccacggcgc
    10081 gaccgagacc gtgcggatca ccggcgccta cgtcatcgcg gcgcccggca acaagggtca
    10141 cctcgtgcgc gacatggtgg ggatgacctt cgagggcacg acgttcgacg accagttcct
    10201 catctgtgac atccgcacca ccttgccggg atgggagttg gagcggcgct tctacttcga
    10261 ccccgagtgg aacccgggcc ggcaggtcct catccacccg tgccccgact cgacctatcg
    10321 gatcgactgg caggtgccgc cgaactacga cctcgcctcc gaggaggcct ccggagcgct
    10381 ggacacccgg atccgtcaga tcgtcggcga cgtcgactac gagatcgtct ggaagtcggc
    10441 ctaccgcttc cacgcccgcc aggtcgacca gatggtcgcc ggtcgccttc tcatggccgg
    10501 tgacggcgcg cacctcgtag cgcctttcgg tgctcgcggc ctcaactccg gcgtccccga
    10561 tgccgagaac gccgcctgga aggtcgcctt cgtcatgcac ggctgggccg acccgtcgct
    10621 catctcgacc tatcacgacg aacggcacgc cgcggctgcc gagaacctcg acgtcaccgc
    10681 cgcgaccatg cgcttcctcg tcccgcagaa cgagcaggag tgggcgagcc gtcgcgagat
    10741 ccttgagggc gcggcagcgc accctgacga cctcgacctg cgggcccagg tcaactccgg
    10801 ccggatggcc gagccgttct ggtatgtcga ctccccgctg accaccccgt cggccgacca
    10861 ccccttcgcc ggacgcccgc ccaagggcga ggacccggtc gccgctccgg gcgtcatcgc
    10921 cccggacgtc gaggtcactc tggccggtgg cgaccgggtc cggatccggg cgctggcccg
    10981 ccgcggcctg ctggtcctcg tcggcgcaga ccttgacgca gaggccgttg ccgaggcgat
    11041 ggccgggcta cgctgccaga cattcgtgtt gcctctggcg gaggtcgacc ctgacggttc
    11101 cgtcgcccag gcattgggtg cccaccccgg cgaggcctgg gtgttccggc ccgatgccca
    11161 catcgcggcg gtggcgacca gcacgaccga cgccgcgtcc gcggcatacc gcgccgtggg
    11221 tgcgaccgcc ccggcgaccg tgtgacctgc cgactgccga ctgccgaccg gccgacctgg
    11281 caacccgccg acctggcact gaccgacccg acgacccgca ccgaccaagg agactccagg
    11341 acatggccta ctaccgcagc gcaggcaaca tcccgcacaa gcggcacgtg cagcaccggg
    11401 accccgaggg gaagctctac tacgaggagc tcatgggtga ggaagggttc tcgtcggact
    11461 cctcgctgct ctaccaccgc ttcatcccgg cggcgatccg cgaggtccgc ccgtggacgc
    11521 tccctgacca gacgttgcgc gccaacgacc cgatgatccc gcggcacttc aagacccaaa
    11581 gcctgccggg tgacggggcc ggcatcgacg cggtcaacgg ccggcgcctg ctgctgggca
    11641 acggcgacgt tcggctgtcg tatgtcgtcg ccaaagaccc gtcgccttac taccgcaacg
    11701 cgattggcga cgagtgcctc tatgtgcacg gcggcacggc gcgggtcgag acggtcttcg
    11761 gcgacctgga tgtctgggaa ggcgactacg tcatcgttcc ccgcgcgacg acccaccgaa
    11821 tcgtcccgac cggagacgcc ccggtgcgga tctacgccat cgaggtgaac agccacatca
    11881 cgccgccgaa gcgttacctc tcgcggttcg ggcagttgct tgagcactcg ccctactgcg
    11941 agcgcgatct gcgggtgccc accgacccct acgtcgtcga ggagaccgac atcccggtgc
    12001 acatcaagca ccgcggccct ggcgccaacg gcatctccgg caccatccac gtccagcccc
    12061 atcacccgtt cgacgtcgtc ggctgggacg gctgcttgtg gccctatgcc ttcaacatct
    12121 ccgactacga gcccctcacc ggccgcgtcc accagccgcc gccgacccac caggtcttcg
    12181 aggcgcacaa cgtcatcatg tgcaacttcg tcccgcgcaa ggtcg
//
LOCUS       DAORPW010000065        12174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1106_length_12174_cov_752.997112, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000065 DAORPW010000000
VERSION     DAORPW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1106_length_12174_cov_752.997112"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLB86_06955"
     CDS             complement(<1..863)
                     /locus_tag="PLB86_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRC64912.1"
                     /translation="MTIRTLHTVHRGDGFHWVGDGFYVTQLLPASPELQRLTDPFLLM
                     DYNPVKEYPPTTTPRGVGVHPHRGFETVTMAFEGAVAHHDSTGAGGVIRPGDVQWMTA
                     ASGILHKEYHEPEWAARGGRFHMMQLWVNLPAAHKMDAPGYQPLTAESMGKVKLDDGG
                     VVTLVAGELDDVRGPARTVTPISLWDVQLGADESADLAVPDGHTLMTFVLDGELTTST
                     GTIGREEMGIFNREGDRLQVTAGEVGARFLVLGGKPIDEPVVFYGPFVMNTMEEIREA
                     MVDFNNGKFGTL"
     gene            complement(926..1267)
                     /locus_tag="PLB86_06960"
     CDS             complement(926..1267)
                     /locus_tag="PLB86_06960"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC64913.1"
                     /translation="MDRTDLYNRYRTAELLFSQRRYAQAALLLEELLQDLQELPEQTG
                     TGEARLLLARAYYHSAQLGRAERAARELVAADPSDPYAVLLLGRVLCRQARGDEGKGW
                     LRRAEALGLTV"
     assembly_gap    1357..1456
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1457..1811
                     /locus_tag="PLB86_06965"
     CDS             <1457..1811
                     /locus_tag="PLB86_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC64914.1"
                     /translation="PEAEPEPGTPECTVVESVLAFLGRAWAGAVVEALLHGNERFGDI
                     AKAIPEATDGVLSARLKDLCARGLALRDVTPGPPVSVRYTLTAAGRDLGPVLDALTAY
                     GATHPEVLIPKRPRG"
     gene            complement(1870..2703)
                     /locus_tag="PLB86_06970"
     CDS             complement(1870..2703)
                     /locus_tag="PLB86_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC64915.1"
                     /translation="MKIERKVFVVTGGGNGIGREVVLGLLTRGARVAIVDRRTDDILE
                     TIRLAGAGAERVSSHIVDVTDEAAVAALPAQVEQVHGQVDGLVNVAGIIQHFVRFADL
                     TEAEMTSVMDVNFWGVVRMCKGFLPLLSARPEACVVNVSSMGAFVPVPGQTVYGASKA
                     AVKLFTEGLYAELRGTSVAVTAVYPGGVATGIAEHSGARIPGAPEADAGGADADAKKM
                     AASLTSPQDAAAAIIGGIERGSYRVVIGKDATMLDRLSRLAPQRATDLVAKKMASLLG
                     H"
     gene            2867..5494
                     /locus_tag="PLB86_06975"
     CDS             2867..5494
                     /locus_tag="PLB86_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRC64916.1"
                     /translation="MPPTAWPFVGREPVVADVRSRLDGDRHVVITGAAGLGKTRVAQE
                     IIDRYAASGTPVVRVVASPASAPVPLAPLAALVGDAIGIEAMNAARRSVAALARQGSS
                     RPLLSVDDLHLLDEASAIVIHQLLTTGTVRVLATVRSSATCPPAVDRLRQSTGVDHVE
                     LGTMADADIADLVERALGGPLSGHARSVIITSAAGNPMYARELVEGSVAAGAMQRHGG
                     VYGFEGRMVATPLLEEVVLARLAPFEGAERTALELLAVGGRLEQPLVERVAGFEPLER
                     LERLGILVAEEHAGRLVLDVGHPLYRELTRARLGALARMRIYRTLAEVDAALANPSGT
                     STPDEQLRAAVWSIRGGLPVESVILTAAAHRAAATGNTPLAAELAHEASRRTGTTQDA
                     MLASWCLSLDGDHDQAIAVLRTALRNETDPWTRAAVRLRIAEEHWWFGDLDSGRAVLD
                     EAAAEPGPWDALREAQLSAHAALAGDVTESVRLGSPLMDHAHPWVRFVAAVGYCLGMI
                     YGDGADEGLRASRALTESLAGTDVQPIGDPGQHHAIQVLGMVAAGELAAARDAADAAY
                     AGAQLSPSQQARAWAAMLVGLVAELAGDLRSGARALAEAERLWTTVRVWGFAVWCGAA
                     LARAQAECGDLDEAATTLARVQAYPRPPQMLNGHLLDLASAWVAGAGGDRVAAAQHIA
                     AAVRHSAAAGAVANEAEAWHEAARLGVLDLVDHLPDPRGWSRPRSALAAARFDLVQAR
                     LTGDPLAWERAASGFAAMGAHGYAAEAATGAAAAYRRAGSSRDAARLDGFAGTHLART
                     TALATPLVARRAATGPLTPREAEIARLAAGHLSNRQIAEQLVVSERTVENHLYRIFIK
                     LAVTGRDDLAAALAATEQG"
     gene            5594..>6282
                     /locus_tag="PLB86_06980"
     CDS             5594..>6282
                     /locus_tag="PLB86_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64917.1"
                     /translation="MAGLDDALARIANDPAWADAVRTQPQEALRGYDLDPQELGRLEH
                     ALGINPGPPAAIFQAPTTGASPAPFAAAPVAASPAASSGVTMAATAIGVGRFPLVVAG
                     LAVASAAIGVGVGSSGALGSPTGPALRADSATVYGCSDDTTRGPAVATLHRGDRVWVI
                     GRSGSSWLVIRHPERLTTPAWISTASVVNSADPATLPELTCSTASAVAAVGPTAIPTG
                     ATSTTSPTAVPT"
     assembly_gap    6283..6382
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6383..6780
                     /locus_tag="PLB86_06985"
     CDS             <6383..6780
                     /locus_tag="PLB86_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64918.1"
                     /translation="SSSSTSSTSSSSSSTPPPDTTGPSLTLTPDSSYLYSDGGAACAA
                     YRQQVTAAVTATDPSGATVDGVTWKAGTLTGSATKVSPGSYRIGPIYSSHSGTIPMTI
                     TVTAHDGKGNSSTRSTTVDFRQIAETCIG"
     gene            6789..9845
                     /locus_tag="PLB86_06990"
     CDS             6789..9845
                     /locus_tag="PLB86_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64919.1"
                     /translation="MTSTPAPQPVSAAPAGAGRHRRVIGALVVLVMLVAGALVGRALR
                     PATPSLPTAYPVLPASSVTARDGRVSPVTAQASSPAGYAVAPAEYVASAAPPPAVAVG
                     AGLTVGAGRPSPNPGGAPDLRADPEAGVSGSAPPSSAPPSAPDLADEAALALVTERYD
                     ELTGATGVRPTATGSTAPSSAPTTSGSSEGGPAYPGIPTDLSKVPLRAFTDPCVLDAS
                     SAACGRGLSAIVLGIRAMPEFQTQWVGEIRRSDLYAGECARQFPTAPIDWASDTIVAA
                     VVNQPVVARLDLQLNTPGVFGSLIRVSHRGAVVTSEAALPSADLIAAWIDAFERGAPT
                     PGIPLCMKVSRQTMETNRDGCLGEFNYNRWLDCSAPFQLSVHTGCSSTSVAPLCSQVG
                     DLLGGREIPQVRQGPFGFQYSYDPQWALWDPQLRAESGVSEANLRRQVRLTPVDALNV
                     EVAIPTFTVTLGEPTAAADPQAPLTGRLALTGHQMVAAAGYAPGTDCRTVDPAIRSTT
                     LPVTRQASATRYWTPDAGIPQSREAVVNLTMPHLAAGEAVDICVFWYQLPSRSFDDIV
                     VTAIERHTVVPPSRAATEIAFVGVGRGGEAPSTPAGSLAAELARWTFQVIGNRTLTAA
                     CGFPSGNTPDPVILHPVGDPGFLCRVEDSSYLFEPRDIELFTTDLAANAPEGRTRLRI
                     DNRGCTPTDCGTPRQARIELSDGRWMELVVRKVPFATAPLLPGGRIATEWSADQWRID
                     PSGFQGVAPTLRTPVRQSAPALDTFGLRVVPTPDSPTDSLDVHWQADREVTLVVLGHP
                     LVPASLAPTCTDTWVEPTAVANSGTVTLPGLCSNTDYGLRVTVTDAATGASQVYDWLR
                     GGARVDVLTGLTFNLVGRTRARPVPPEFLAAWSVSVAEPTGSTSPTGAGTGAVEGTLA
                     ALSVRVGEHTLVSRTDPATTCTRVAPGTTLASGFDGGQPLGQLRVAVSFTLVAPGTDG
                     CAGPPGTTNFSVQQDVIWDGRSEQTFRLSAPDGRTVALTVRPATRD"
     gene            9896..10558
                     /locus_tag="PLB86_06995"
     CDS             9896..10558
                     /locus_tag="PLB86_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64920.1"
                     /translation="MKDILPGRGRRAGVVTLALALVTSVTGLAGLAGCGSGGGSTTSA
                     AASTAGGAAAQSPAVQSPTAAAPATTPAGGTGASNGSGSQPTWCQPPQGPMEILLGKG
                     TVFEITGDDKRCRFAATGGKRGVVDVSNLTAFDPTVTYEATRKGERAVRCNAPLVEPA
                     GVGEQSWLNATCFAQTFTINLYAAKAGTIYEFIYTGDPSIPVTAAQASTELLQVAKVI
                     LH"
     gene            10653..11912
                     /locus_tag="PLB86_07000"
     CDS             10653..11912
                     /locus_tag="PLB86_07000"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HRC64921.1"
                     /translation="MGMPSHRLRPMTGRDPNEQSRASSTLELLFDLTVVIGLGTAAGQ
                     LAHYLAEGHATVAVIGFLMSAFAVVWAWMNWSWFASAYDTDDWAFRLLTLVQMVGALI
                     LSLGVPAVFSSFEHGGQIDNQTMVSGYVVMRVPMIIQWLRAARSDPAHRGICLVYAQT
                     LAAAQTGWVIVAIAPLTFWPSLLLVLALASTELLGPILLSRRHGGTPWHPHHIAERYG
                     LLIIIALGEGLIGTMATLSVLFDASGWSVDFVVVGLAGVGLTFGIWWAYFLFPSGALL
                     EHFRSRSRLWGNGHIPLFIAVVAVGGGLHLAAYAIDGRSHLGPVPTLLAIAIPLAAYA
                     VLIYALFGSLTRALTRRHLWQLAASLAVALLSVVLVALGSPLSVGLVVLSLVPWVTVI
                     AQELDARRKELSSDALALGASPHHRRV"
     gene            complement(11995..>12174)
                     /locus_tag="PLB86_07005"
     CDS             complement(11995..>12174)
                     /locus_tag="PLB86_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRC64922.1"
                     /translation="DYKATDFRMAELSWLWGVNRNILTPPGLDEQWLVSLAHTDEDMA
                     ALVSDFRDLAVALRA"
BASE COUNT         1751 a         4398 c         4036 g         1789 t
ORIGIN      
        1 agcgtgccga acttgccgtt gttgaagtcg accatcgcct cgcggatctc ctccatcgtg
       61 ttcatcacga acggaccgta gaacacgacc ggctcgtcga tcggcttgcc gccgagcacg
      121 aggaaccgag cgcccacctc gccggcagtg acctgcagac ggtcaccctc gcggttgaag
      181 atgcccatct cctcacggcc gatcgtccct gtcgacgtcg tcagctcacc gtcgagcacg
      241 aacgtcatca gcgtgtgacc gtcgggcacg gcaaggtcgg ccgactcgtc ggcaccgagt
      301 tgcacgtccc acagcgagat cggggtgacg gtgcgagcag gcccgcgcac gtcgtcgagc
      361 tcaccggcga cgagggtcac cacgccaccg tcgtcgagct tgaccttgcc catcgactcg
      421 gcggtcagcg gctggtaacc cggcgcgtcc atcttgtgcg ccgcaggcag gttgacccac
      481 agctgcatca tgtggaaacg cccaccgcgg gccgcccact caggctcgtg atactccttg
      541 tggagtatgc cgctcgcggc cgtcatccac tggacgtcac ccgggcggat gacgccaccg
      601 gcgccggtcg agtcgtggtg ggcgacagcc ccctcgaagg ccatcgtcac cgtctcgaag
      661 ccccggtgcg ggtgcacgcc gaccccacgg ggagtcgtgg tcggcgggta ctccttgacc
      721 gggttgtagt ccatgagcag gaacgggtcg gtgagccgct gcagctcggg cgacgccggg
      781 agcaactggg tcacatagaa cccgtcgccg acccagtgga atccgtcgcc acggtggacg
      841 gtgtgcagag tgcggatggt catgacagaa acctctcaga gaagcagttg ttggtcgggg
      901 gattcagcgt ggtgatgggg gcgggttaga cggtgaggcc gagggcctcg gcgcggcgca
      961 gccagccctt gccctcgtcg ccccgcgcct gccggcacag gacgcgcccg aggaggagca
     1021 cggcatacgg atcgctcgga tcggcagcga ccaactcgcg agccgcacgc tccgcgcggc
     1081 ccagttgggc cgagtggtag taggcgcggg ccagcagcag gcgcgcctcc ccggtgccgg
     1141 tctgctcggg cagctcctgc agatcctgga gcaactcctc gagcaggaga gcggcctggg
     1201 catagcgacg ctgcgagaag agcagctcag cggtgcggta acggttgtag aggtcagtgc
     1261 ggtccatgcg gtcgtccatg gcactatcaa catcatagtg actcggatat tcccaggcac
     1321 tatgagactc ggggtagact gatgcccaac acccgannnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnngccc gaagccgagc ccgaacccgg cacccctgag tgcacggtgg
     1501 tcgagagcgt cctggccttc ctcggccgcg cgtgggcagg cgccgtcgtc gaagccctgc
     1561 tgcacgggaa cgagcggttc ggcgacatcg cgaaagcgat ccctgaggcg accgacggag
     1621 tgctttcggc gcgcctcaag gacctgtgcg cccgcggcct ggccctacgc gacgtgaccc
     1681 cgggcccgcc ggtcagcgtc cgctacaccc tcaccgcggc cggccgtgac ctcgggccgg
     1741 tgctagatgc gctcacggca tacggagcca cccaccccga ggtgctgatc cccaagcgac
     1801 cccgcggctg aagtctcgcg agcgcggagc acccgtcagg cgagggtatg ccgccgcacc
     1861 gccccggggt tagtgcccga ggagggaggc catcttcttg gcgaccaggt cggtggcccg
     1921 ctgcggggcg agccgcgaga gccggtcgag catcgtcgcg tccttgccga tcaccacgcg
     1981 atagctgccg cgctcgatgc cgccgatgat cgcagcggcg gcgtcctgcg gcgaggtgag
     2041 gctggcggcc atcttcttcg cgtcggcgtc ggccccgccc gcatcggcct ccggggcgcc
     2101 cgggatccgc gcccccgagt gctcggcgat acccgtcgca acgccgcccg gatagacggc
     2161 ggtgacggcg accgacgtgc cgcgcaactc ggcatacagg ccttcggtga agagcttgac
     2221 cgcagccttg ctcgcgccgt agaccgtctg ccccggcacc ggcacgaacg cgcccatgct
     2281 cgacacgttg accacacagg cctccgggcg ggcggacagc aacggcagga accccttgca
     2341 catccgcacg acgccccaga agttgacgtc catgaccgag gtcatctcgg cctcggtgag
     2401 atcggcgaac ctcacgaaat gctggatgat cccggccacg ttgaccagcc cgtcgacctg
     2461 gccgtgcacc tgctcgacct gcgccggaag ggccgccact gcggcctcgt cggtgacgtc
     2521 gacgatgtgg ctgctcaccc gctcggcgcc cgcccccgcc agccggatgg tttccagaat
     2581 gtcgtcggtt cgccggtcga cgatagccac gcgagcgcct cgggtgagga gcccgaggac
     2641 cacctcccgg ccgatcccat tgccgccccc ggtgaccaca aacaccttgc gctcgatctt
     2701 catgatcgat cccttcgtcc ccagccgagt ctcttccggg gctcacctca acgctaactc
     2761 tgttgtcgca caccggcggt cgtccagcgt gtctcgtcgc tcacctctgg ccgcgccggt
     2821 aacccagaac acggacgggc cggctctcac gccctaaagt gctgccgtgc ctcccactgc
     2881 ctggccgttc gtcggacgcg agcccgtggt cgccgacgtg cgctcgcggc tcgacggcga
     2941 ccgccatgtg gtcatcaccg gggcagcggg cctgggcaag acccgagtgg cccaagagat
     3001 catcgacagg tatgccgcgt ccggcacccc cgtcgtgcgg gtcgttgcca gccccgcgtc
     3061 cgcgccggtg ccgttggccc ccttggcagc cctcgtggga gacgcgatcg gcatcgaagc
     3121 gatgaacgcc gcccgacggt cggtcgccgc gctggctcgg caaggctcgt cacgtcccct
     3181 gctgtcggtc gacgacctgc acctgctcga tgaggccagc gccatcgtca tccaccaact
     3241 gctcacgacg ggcaccgtgc gcgtcctggc caccgtccgc agctccgcca cctgcccacc
     3301 tgcggtggac cggctgcgcc agtcgaccgg ggtcgatcac gtcgagctgg gaaccatggc
     3361 agacgccgac atcgccgacc tcgttgagcg cgccctcggc ggcccgctgt ctgggcacgc
     3421 gcggagcgtc atcatcacct cggcggccgg taaccccatg tatgcccggg aactcgtcga
     3481 ggggtctgtc gcagctgggg ccatgcagcg ccatggcggc gtctatgggt tcgagggccg
     3541 catggtcgcc acgccgctgc tggaagaggt ggtgctcgcc cgcctggcac cgttcgaggg
     3601 tgcggagcgg acggcgttgg agttgctcgc cgtcgggggt cgcctggaac agccactcgt
     3661 cgagcgggtc gccggcttcg agccgttgga gcgcctcgaa cggctgggca tcctcgtggc
     3721 cgaggagcac gccgggcgac tcgtcctgga cgtcgggcat cccctctatc gcgagctcac
     3781 ccgggcgcgc ctgggagcgc tagcccggat gcggatctac cgcaccctcg ccgaggtcga
     3841 cgccgctctg gccaacccct ccggcacgag caccccggac gagcagttgc gggctgccgt
     3901 ctggagcatc cgcggcgggc tgccggtcga atcggtgatc ctcacggcgg cggcccatcg
     3961 ggcggccgcc acgggtaaca ccccgctagc ggccgagctc gcccatgagg caagccgacg
     4021 caccggcacc acccaggacg ccatgctcgc gtcgtggtgt ctgtcgctcg acggcgacca
     4081 cgaccaagca attgccgtcc tgcgcacggc tctgcgcaac gagaccgacc cgtggacccg
     4141 cgccgcggtg cgcctgcgca tcgcggagga acactggtgg ttcggcgacc tcgacagcgg
     4201 tcgcgcggtg ctcgacgagg cggccgcaga accgggccct tgggacgcgc tgcgggaagc
     4261 acaactgtcc gctcacgccg cccttgcggg cgacgtgaca gagtcggttc ggttggggtc
     4321 accgctcatg gaccacgccc atccctgggt gaggttcgtt gccgccgtgg gctattgcct
     4381 cgggatgatc tacggcgacg gtgccgacga agggttgcgg gcatctcggg cactcactga
     4441 gagcctcgcc ggcaccgatg tccagcccat cggggatccg ggccagcatc acgccatcca
     4501 ggtgctcggc atggtggccg ccggcgaact agccgcggcc cgggacgctg cggacgcggc
     4561 atacgccggc gcccaactgt ccccctcgca acaggcgcgc gcgtgggcag cgatgctcgt
     4621 cggtttggtg gcggagttgg ccggcgacct gcgatcgggg gcccgcgcgc ttgccgaagc
     4681 cgaacggttg tggaccacgg tgcgagtgtg gggattcgcg gtgtggtgcg gagccgcgct
     4741 cgcccgggct caggccgagt gcggagacct cgacgaggcg gccaccacgc tggctcgggt
     4801 gcaggcctac cccagaccac cacagatgct caacggccac ctcctcgacc tggccagcgc
     4861 gtgggtcgcc ggcgccggag gcgaccgagt cgctgccgcc caacacatcg ccgccgcggt
     4921 gcgtcactcc gcagctgccg gggcggtcgc gaacgaagcc gaagcctggc acgaagccgc
     4981 ccgactcggg gtcctcgacc tcgtcgacca cctccccgac ccgcgcggct ggtcccgccc
     5041 acgctcggcg cttgcggcag cccgcttcga cctcgtgcag gcccggctga ccggcgaccc
     5101 cctcgcgtgg gaacgcgccg cttccggttt cgccgccatg ggcgcccacg ggtatgccgc
     5161 ggaggccgcg accggggcgg ccgcggcata ccgtcgggcc ggatcgagcc gagacgccgc
     5221 gcgccttgac ggcttcgcag ggacccacct cgcccgcacc acggccctgg ccacccctct
     5281 cgttgcgcgc cgtgccgcga ccgggccgct gaccccacga gaggccgaga tcgcccgcct
     5341 cgccgccggc cacctgagca atcgccagat cgccgagcag ttggtcgtca gcgagcgcac
     5401 cgtcgagaac cacctctatc ggatcttcat caagcttgcg gtcacggggc gggacgacct
     5461 cgcagcggcg ttggctgcca ccgagcaggg ctgaggcggc ccggtcgggc ctggggggat
     5521 cgagtacccc cgacggtggg ctgagtagtc gccactcatg cggcgacagg cgtgcgccga
     5581 cgaccgtgtg gtcatggccg gactcgacga tgccctcgcg cgaatcgcca acgaccccgc
     5641 gtgggccgac gctgtgcgca cccagcccca ggaggcgttg cgcggctacg acctggatcc
     5701 gcaggagctg ggtcgccttg aacatgccct cggcatcaac ccagggcctc cggctgccat
     5761 cttccaagcg ccaaccaccg gcgcctcccc cgccccgttc gccgcagctc cggtcgccgc
     5821 cagtcccgcc gcctcgagcg gggtcacgat ggccgcaacc gcgatcggcg tcgggcggtt
     5881 cccgctcgtc gttgctggcc tcgccgttgc gagcgccgcc atcggcgtgg gcgtcggctc
     5941 cagcggagcg ctcggctccc ccaccggccc ggccctccgt gcggactcgg ccacggtcta
     6001 cggctgcagc gacgacacga cccgcggtcc ggccgtcgcc accctgcacc gcggcgaccg
     6061 agtctgggtc atcggccgca gcggcagctc atggctggtc attcgtcatc ccgagcgact
     6121 gaccaccccg gcgtggatct ccacggcgag cgtggtgaac tccgccgacc cggcgaccct
     6181 gcccgagctc acctgcagca ccgcgagcgc ggtggccgcc gtcgggccga ccgcgatccc
     6241 gaccggcgcg acatcgacca cgagccccac ggccgtgccg acnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nngctcgtcg tccagcacat ccagcacctc gagctcctcc
     6421 agctcgaccc caccaccgga caccaccggc ccgagcctca cgctcacccc ggactccagc
     6481 tacctctact ccgacggtgg cgccgcgtgc gcggcatacc gccagcaggt gaccgccgcg
     6541 gtgacggcga ccgacccgag cggcgcgacc gtcgacgggg tgacctggaa ggcggggacg
     6601 ctcaccggga gcgctaccaa ggtgtcgccc ggcagctacc ggatcggccc gatctacagc
     6661 tcccacagcg ggaccatccc catgacgatc accgtgacgg cccacgacgg caagggcaac
     6721 tcctcgaccc gatccacgac cgtcgacttc cgccaaatcg ccgaaacctg catcggctga
     6781 ggagcgccat gaccagcacc cctgcccccc aacccgtgtc cgcagccccc gcaggagcgg
     6841 ggcgacaccg ccgcgtgatc ggcgcgctcg tcgtcctcgt catgctcgtc gctggcgccc
     6901 tggtcgggcg cgcgctgcgc cccgcgacgc cgagtctgcc cacggcatac cccgtgctcc
     6961 cggcgtcgtc ggtcaccgcg cgcgacggga gggtctcgcc cgtcacagcg caggcgagca
     7021 gccctgcagg gtatgccgtc gcgcctgcgg agtacgtcgc cagcgccgca ccgccgccgg
     7081 cggttgcggt cggtgccggg ctgacggtcg gcgccgggcg ccccagcccc aaccccggcg
     7141 gcgctcctga tctgcgggcc gatccggagg cgggggtgag cgggagtgcc ccgccgagca
     7201 gcgccccgcc gtcagcaccc gatctggccg acgaggcggc actggcgctg gtgaccgagc
     7261 gctacgacga attgaccgga gccaccgggg tgcgcccgac ggccactggg tcgaccgcgc
     7321 cgtccagcgc ccccacgacg agtggctcca gtgagggagg cccggcgtat ccggggatac
     7381 cgactgacct gagcaaggtt ccgctgcggg ccttcaccga cccgtgcgtc ctggacgcct
     7441 cgtctgcggc ctgcggtcgg ggcctgtcgg cgattgtcct ggggatccgg gcgatgccgg
     7501 agttccagac ccagtgggtc ggtgagatcc gtcgcagcga cctgtatgcc ggtgagtgcg
     7561 cccggcagtt cccgacagcc cccatcgatt gggcgagcga cacgatcgtc gctgccgtcg
     7621 tcaaccaacc ggtcgtcgca cggctggacc tgcaactcaa cactcctggc gtcttcggct
     7681 cactcatccg ggtgtcgcac cggggtgcag tggtcaccag cgaggccgcc ctcccctcgg
     7741 ccgacctgat cgcggcctgg atcgatgcgt tcgaacgtgg ggcgcccacg ccgggaatcc
     7801 cgctgtgcat gaaggtgtcc cgccagacga tggagaccaa ccgagatggg tgcctcggtg
     7861 agttcaacta caaccggtgg ctcgattgct cggcgccgtt ccaactgtcg gtgcacaccg
     7921 ggtgctcgtc cacgtcggtc gcaccgctgt gcagccaggt gggcgacctg ctcggaggtc
     7981 gcgagatccc gcaggtccgt caaggaccct tcggcttcca atactcctat gacccccaat
     8041 gggcattgtg ggacccgcag ctgcgcgcgg agtccggggt ctccgaggcc aacctccgtc
     8101 gccaggtgcg gttgactccc gtggacgccc tcaacgtcga ggtggccatc cccaccttca
     8161 ccgtgaccct cggcgagccc accgccgcag ccgacccgca ggctccgctc accgggcgac
     8221 tcgcgctgac cggacatcag atggtcgccg cggccggcta tgcccccggg accgactgcc
     8281 gcaccgtcga cccggccatc cgcagcacga cgttgccggt gacccgccag gcgagtgcga
     8341 ctcgctactg gactccggac gcaggcatcc cgcagagccg cgaagccgtc gtcaacctga
     8401 cgatgccgca cctggccgcc ggcgaggccg tcgacatttg cgtcttctgg taccagctgc
     8461 cgagccgctc cttcgacgac atcgtcgtga cggcgatcga gcggcatacc gtcgtgcctc
     8521 cgtctcgggc ggcaaccgag atcgcgttcg tcggggtggg tcgcggcggt gaggcgccca
     8581 gcacgccggc aggttcgctg gccgcggaac tggcgcggtg gaccttccag gtgatcggca
     8641 atcggaccct cacggccgcg tgcggattcc cctcgggcaa cacccccgac ccggtcatcc
     8701 tgcaccccgt cggcgacccc gggttcctgt gtcgggtgga ggacagcagt tacctgttcg
     8761 agccgcggga tatcgagctg ttcaccactg acctggccgc caatgcgccg gaggggcgca
     8821 cccggctgcg gatcgacaac cgaggctgca caccgaccga ttgcgggacc ccccggcagg
     8881 cgcggatcga gctcagcgac ggccggtgga tggagttggt cgtgcgcaag gtgccgttcg
     8941 ccactgcgcc gctgctgccg ggtggtcgga tcgccactga gtggtcggca gaccagtggc
     9001 gcatcgaccc ctcgggattc cagggcgtgg caccgacgct gcgcactcca gtccggcaga
     9061 gcgcaccggc cttggacacc ttcggcctgc gggtcgtgcc gactccggac agcccgaccg
     9121 actcgttgga cgtgcactgg caggccgatc gagaggtcac cctggtcgtt ctcggccatc
     9181 cgctcgtccc cgcgagcctg gccccgacct gcacggacac ctgggtggag ccgaccgccg
     9241 tcgccaactc gggcacggtg acgcttcctg ggctgtgttc caacaccgac tacggcctgc
     9301 gcgtgacagt caccgacgcc gcgaccggag cgtcgcaggt ctatgactgg ctccggggtg
     9361 gagccagggt cgatgtgctg accgggctga ccttcaacct cgtcggacgc acccgagccc
     9421 ggcctgtccc gccggagttc ctggccgcgt ggtcggtcag cgttgccgag ccgaccgggt
     9481 cgacttcccc gacgggtgcc ggcacgggtg cggtcgaggg aaccctggct gccctctcgg
     9541 tccgggtcgg cgagcacacc ctggtgtccc gcaccgatcc ggcgaccacc tgcacgcgag
     9601 tcgcccccgg cacgaccctt gccagcgggt tcgacggtgg ccagccactg ggtcagttgc
     9661 gggtcgccgt gtccttcact ctcgtggcac ccgggaccga cggctgtgcc ggaccgccgg
     9721 gcaccacgaa cttcagcgtg caacaggacg tgatctggga cgggcgcagc gaacagacgt
     9781 tccggctgag cgctcccgat ggccgcaccg tcgcgctcac cgtgcgccct gccacacggg
     9841 actagacgaa acgaattccc ttgatgtcag aagaaattca ccgaaggaga acgctatgaa
     9901 ggacatcctg ccgggccgtg gtcggcgagc cggcgtcgtg accctcgcgc tcgcgctcgt
     9961 caccagcgtg accggcctgg ccggcctagc ggggtgcggg tccggtggcg ggtcgaccac
    10021 ctcggccgcc gcatcgacag ccggcggcgc ggccgctcaa tccccagcgg tccagtcccc
    10081 cacggcagcg gcgcctgcaa cgacccccgc gggaggaacc ggcgccagca acggctcagg
    10141 cagtcagccg acctggtgcc agcccccgca aggcccgatg gagatcctgc tcggcaaggg
    10201 caccgtcttc gagatcaccg gcgacgacaa gcggtgtcgg ttcgcggcaa ccggcggcaa
    10261 gcggggagtc gtggacgtca gcaacctcac cgccttcgac cccacggtca cctacgaggc
    10321 gacccgcaag ggcgaacgag ccgtccgctg caatgccccg ctcgtcgagc ccgcaggcgt
    10381 gggagagcag tcctggctca acgccacgtg tttcgcgcag accttcacga tcaacctgta
    10441 tgccgccaag gccggcacga tctacgaatt catctacacg ggcgacccgt cgatcccggt
    10501 gacggccgcc caggccagca ccgagctgct tcaggtcgcc aaggtcatcc tgcactgacc
    10561 acggggacgc ccacgagcgg cggcgcgcgg catacccagg gggtatgccg cgcgcctcga
    10621 gcagcggtga gtcgactcct tacactgctc ggatgggtat gccgtcgcac cgcctccggc
    10681 cgatgaccgg ccgggatccc aacgagcaaa gccgcgcgtc gagcacgctc gagttgcttt
    10741 tcgacctcac cgttgtcatc ggattaggca ctgctgcagg acaattggcg cactacctcg
    10801 ccgaggggca cgcgacggtc gcggtgatcg gcttcctcat gtcggcgttc gcggtcgtgt
    10861 gggcctggat gaactggtcg tggttcgcgt cggcctatga caccgacgac tgggcgttcc
    10921 gactgctcac cctcgtgcag atggtcggcg cgctgatcct ctcgctcggc gtgccggccg
    10981 ttttctcgtc gttcgaacac ggcggccaga tcgacaacca gaccatggtg agcggttacg
    11041 tcgtcatgcg cgtgccgatg atcatccagt ggttgcgcgc cgcccggtcc gatccggccc
    11101 atcgcggcat ctgcctggtc tatgcccaga cgctcgccgc cgcgcaaacc gggtgggtga
    11161 tcgtcgctat cgctcccctg acgttctggc ccagcctgtt gctcgtgctt gctctcgcca
    11221 gcacggaact gctcgggccg atcctcctca gccggcgaca cggcgggacg ccctggcatc
    11281 cgcatcacat cgccgagcgt tacgggctgc tcatcatcat cgccctcggc gagggcctca
    11341 tcggcacgat ggccacgctg tcggtgctgt tcgacgcgtc cgggtggtcg gtcgacttcg
    11401 tcgtcgtcgg gctcgccgga gtcgggttga ccttcggcat ctggtgggcc tacttcctct
    11461 tccccagcgg cgccctcctg gagcacttcc gcagccggtc ccggctatgg ggcaacggcc
    11521 atatccccct cttcatcgcg gtcgtggccg tcggcggcgg actgcacctc gcggcatacg
    11581 ccatcgacgg acggtcgcat ctgggtccgg tgccgaccct cctcgcgatc gcgatccccc
    11641 tcgccgcgta tgccgtgttg atctacgcgc tgttcggctc actgacgcgg gccctcactc
    11701 gcaggcatct gtggcagttg gccgcgtccc tcgcggtggc gctgctcagc gtggtcctgg
    11761 tggcgctggg ctcgccgctg tcggtggggc tggttgtgct ctcgctcgtg ccgtgggtga
    11821 ccgtgatcgc gcaggagttg gacgcccgtc gcaaggaact cagcagtgac gccttggccc
    11881 tgggggcttc cccacatcac cggcgggtgt gagggaatcc gggacgccag ggcccggcga
    11941 gctggaaatc accggcgggt gtgaggaaat ccggaaagag ggcggggcag cgggtcaggc
    12001 gcggagtgcc accgccagat cacggaagtc gctgaccaac gccgccatgt cctcgtcggt
    12061 gtgcgccagc gaaaccagcc actgctcgtc cagcccgggc ggcgtgagga tgttgcggtt
    12121 gacgccccag agccaggaca actcggccat ccggaagtcg gtcgccttgt agtc
//
LOCUS       DAORPW010000066        12167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1107_length_12167_cov_651.613276, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000066 DAORPW010000000
VERSION     DAORPW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12167
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1107_length_12167_cov_651.613276"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLB86_07010"
     CDS             <1..543
                     /locus_tag="PLB86_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HRC64923.1"
                     /translation="DADGGDDEASGPSAGRSRGSRSRSRVALLEREGEVAADFLERLL
                     DIADLDGDIEVDIDGDRAAVSIVDSEEGRVPRRLVGPDGKVLDALQELTRLAVQTATG
                     ERSRLMLDVAGFRADRRTALVTLAQEAIAEVRASGVARALDNMSAFERKVVHDEVTAA
                     GLVSESEGVEPHRHIVISPA"
     gene            540..1313
                     /gene="rsmG"
                     /locus_tag="PLB86_07015"
     CDS             540..1313
                     /gene="rsmG"
                     /locus_tag="PLB86_07015"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRC64924.1"
                     /translation="MTEVDVPVPPLSAAQVFGPREELARAYVGLLADTGISHGLIGPR
                     EVPRLWDRHVLNCAVIQELLPPNALCADIGSGAGLPGLALAIARPDITMHLVEPLQRR
                     ARWLDVAVAELGLSGVTVHCSRADALHGVLTVDVVTARAVAELATLGQWCLPLLPPGG
                     RLLAIKGSKAEAELAEAWPALQRLGCQAGSVRECGGSMIDPPTRVVEVIAGSAPSSGK
                     GRARPPSARAQQRKARAAAARRHRGGFSPTGADEASPSN"
     assembly_gap    1582..1681
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1682..2608
                     /locus_tag="PLB86_07020"
     CDS             <1682..2608
                     /locus_tag="PLB86_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRC64925.1"
                     /translation="DPAAESSSLVDLQDESTPLAAAIAQDVRRRSTLVGAPLPRPPAT
                     RVLTVANQKGGVGKTTSAVNVAAALAQSGMRVLVIDLDPQGNASTALGVDHRAGIDSI
                     YDVLVDGQPMSAVVVESPTVPGLFCAPSTIDLAGAEIELVSLVAREGRLATALNAHLA
                     DTAYDYVFIDCPPSLGLLTVNAFVAAREVFIPIQCEYYALEGLSQLLRNVELIRTHLN
                     PTLAVSTILLTMFDGRTRLSAQVAEEVREHFPEQTLRTTIPRSVRVSEAPSFGETVMT
                     YDPLSSGALAYLEAASQIAQRGAESMNEGAHP"
     gene            2605..3747
                     /locus_tag="PLB86_07025"
     CDS             2605..3747
                     /locus_tag="PLB86_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRC64926.1"
                     /translation="MKEREKRRALGRGLGALIPTAPAGERPVDVFFGPAPSAITVDLG
                     TKHAAADGDPSDLTDSAETTDPTDAAAASSSSSPTDAPSAGTGPADPDNDALRPVPGA
                     TFAELPVESIRPNPRQPRTVFDEDDMAELVHSVSEIGILQPIVVRRVDDGGPLGSGHD
                     AAIATYELVMGERRWRAAQAAGLEHVPAIIRSTHDDDLLRDALLENLHRSQLNPLEEA
                     AAYRQLLDDFGCTQEELATRIGRSRPQVSNTLRLLKLPPLVQRRLAAGVLSAGHARAL
                     LGLTDAAAMERLAQRIVAEGLSVRSVEEIVAVGAVGTAPAKPKSPRAGGRRPQLDDMA
                     ASLSDHLDTRVTIALGQRKGKVVVEFASVEDLNRILDVIGVDRPKR"
     gene            3754..4425
                     /locus_tag="PLB86_07030"
     CDS             3754..4425
                     /locus_tag="PLB86_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC64927.1"
                     /translation="MAATPKRRKGGPEIVPVTADDVPALVEPCRDCAFWERGPGLVAG
                     TASTVSRDKAAWVRRVEAEWGTPGWLVRVDGQPAGHLLIAPAHLAPRSLAFPTSPVAD
                     DAILLLAVRVDPRYAGQGLGRRLVQTAAREVLSHDGRAIEAFGTTRGDQCLTPVDFLT
                     AVGFYVVREHAAYPRLRLDLRTALAWREDVEHALDRILAPVRRLGAGNPVGTANNEAP
                     GAGRP"
     gene            complement(4456..4782)
                     /gene="trxA"
                     /locus_tag="PLB86_07035"
     CDS             complement(4456..4782)
                     /gene="trxA"
                     /locus_tag="PLB86_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRC64928.1"
                     /translation="MGAIAATTDSTFATDVLANTKPVLVDFWAAWCGPCKQIAPILEE
                     IYAEYGDKIEIVKLDTDNNPATTARYGVTGIPTMNLYVGGEVVKTIVGAKPKRLLLRE
                     LEQFLG"
     gene            complement(4896..5885)
                     /gene="trxB"
                     /locus_tag="PLB86_07040"
     CDS             complement(4896..5885)
                     /gene="trxB"
                     /locus_tag="PLB86_07040"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRC64929.1"
                     /translation="MSDAVETATDTGVREVVIVGSGPAGYTAAVYAARANLHPVLFEG
                     SVTAGGALMNTTEVENFPGFEDGIQGPDLMFAMRAQAEKFGTEMVTDDIVAMDLAGDI
                     KTVTDGEGRVWRAKSVILAMGSAYRELGLPEEKRLSGHGVSWCATCDGFFFRDQDIAV
                     VGGGDSAIEEATFLTKFARSVTIIHRRDQLRASKIMADRALANPKIRFTWNAAVTALH
                     GDPKLTHVTLSDTVTGATSDLQVTGLFVAIGHDPRNELIRGVIDLDDAGYVVCEGRST
                     RTNLPGVFACGDLVDHTYRQAITAAGSGCAAALDTQHYLEALSDVAESVAAAH"
     gene            complement(6112..7083)
                     /locus_tag="PLB86_07045"
     CDS             complement(6112..7083)
                     /locus_tag="PLB86_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64930.1"
                     /translation="MSEPTRFDEDPDPTGVRILLASQPDPGPMPADVSDRILAAFAEA
                     GRRADLDADTGSQELSDELGLGVDAFDDFANLVAGQGVSGVDESGPAVPLHLVGRGTQ
                     PAGTSSETSGARTDVRTGSRSGSRSGAGMRGRVVDHRRRLTVLGGVAASVAALGLVGA
                     LLNNQGATSPTASSARAESVVGAAKDSSSLSAPADSATRANAATAVNANGQPMHIELS
                     TRTYTKATLARLAQDMLDAPGRQLGQPAVEAPSVGPIGTLIGLTDCVTTLGESDADAV
                     FADLATFEGAPAVVIVVVDAELKQAYAVSRSCSKGDPGLLFGPVDMP"
     gene            complement(7208..7753)
                     /gene="sigM"
                     /locus_tag="PLB86_07050"
     CDS             complement(7208..7753)
                     /gene="sigM"
                     /locus_tag="PLB86_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HRC64931.1"
                     /translation="MTSGRVELAGRSDAELLAAHCAGDREAFAEIFRRHKDRMWAVAV
                     RTCGDPELASDCVQDAFIAAYRRAGSFRGESAVTTWLHRIVVNACLDRLRRTRPTSPL
                     PDYDLADRSDAHSAVEVRLDVRAALDQLPEGQRAALVLVDMHGLSVAETAEILGVAEG
                     TIKSRCARGRAAMAQLLGIRR"
     gene            complement(7750..9978)
                     /locus_tag="PLB86_07055"
     CDS             complement(7750..9978)
                     /locus_tag="PLB86_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64932.1"
                     /translation="MQHVGPGTLIGDRYAVSHRLQQHPRWERWAAHDTTLGRDVVILT
                     FAGGTPAAAAAVDAARRAAAVTEPRLVRVLDIVTPPTTDAQVPSSPSGSPSEAAGGSA
                     GGPASAAIVCAIIEEPVERSRSLTRILEQGGLPADEARRITGEAAMALDAAAARGLRH
                     IVLTPRNVLLLPDGSVRVRGVAVEAALLGLEDTPADVGARLDATALVSIGYAALTGRW
                     PLGGADSGLPRAPVVGGTVVAADELGADACSDLQRLARATFVEHAGPSTSADVLAGLR
                     PWPKGPTVDLWGDRRPPAVPSATEGQGAGPRAGQGAVAGGGTAAGAGPAAPSLVQPDS
                     PAAQPFSSQQQPSSSAGGGATAEGVSLATGAGGAAGGAGATSAAATAAAVSAAMAKGA
                     RGVGSALGAAGTAASQAASRLGGRARHTVERLSTRPEPATDEGIASAPAAPGAGAPEA
                     SSAQPPGSPGSGTPTAPGPSAPRVAPGAPRVVAPRTLRAPDLIREDATLASVLAPVDS
                     PLEDAVPLVPITGELGRDESRLALAIVAGLVVLLAVLGIWGLPKLTSGGTTGGSVTTA
                     RVTRTATVTSSAGATGAGATAGDGPAVVASLTPVAIIGAATFDASAGQVASSSAPRGY
                     DGNAATMWRSKWYSNEKFGGLKVPGIGYIVDLGQQTEVRRVTVTLPAPQDITVYVANR
                     ASLDGATAIGSSTGQSGQLVFDAPAGPVSSGQLVIVFVTKLGPDGAGNFRAQVSEVAV
                     SR"
     gene            complement(10036..>12167)
                     /locus_tag="PLB86_07060"
     CDS             complement(10036..>12167)
                     /locus_tag="PLB86_07060"
                     /inference="COORDINATES: protein motif:HMM:NF039636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HRC64933.1"
                     /translation="ATPATATTATATTATATVTIPRADPSGDRITLTAVSPAVLRPGD
                     TLLVTGTIDVTSTAADGIPTTVRLVRGRSSLTSRADVDEWSAATGAVSGTELGRQPLD
                     PAAPRNAIPFQFTVPADQVRPGRAYGVSPIAVDVVTAKGTSVSALRTFVGWQRSADYE
                     ALSLAWLLPVTLAPDTALTADDPTARTAAWTKQVGAGSRLAALLRATASQTVGYAVDP
                     AVLGPSGRTPAQDAADPASAVRSAFSMSLSAASATHPVFALPQNDPDLAALTAPDLGP
                     TLSPAASQLLAEAVGQSTRLTDAAVTTRGTLAWPADGALPSGREAALRAAYASRLDAI
                     LVSTQVTDPSARLTPPALGVAPLGTTVLRWDDRLGALLARLKTPREAVLAAQEFVAQT
                     AALLAERPSISRTILAVPPRGFDAGLDADLVSTFFDATKQVPWLRTVGVDSAMLPGRG
                     LDSAPPLPTDAPASRVTPTDPASAAASNGPAAGRSLLARVFQQRSVIDQLSSVLAQGS
                     PTIAAWSDLPGQAASTRWRTDPAGREALLAGLEQSTATVSNGLMVIPQTTNFLADEGV
                     MQLTVVNSLDEPVRGLRAVLAPGNGRIVVVDPGAPVDIAANAKATVSVRLAVLAQGLV
                     PVNAWLTTADGTRIGAVQQLTIRAAPPGAWLYIGMSVLFGLILIAGIVRAVRRPPRMV
                     VDTRGLDPVDPTPEAPVVAPVQRHTAP"
BASE COUNT         1799 a         4165 c         4343 g         1760 t
ORIGIN      
        1 gacgccgacg gtggcgacga cgaggccagc ggtccgtccg cgggtcggtc gcgtggttcg
       61 cggtcgcggt cccgcgtggc tctgctcgag cgtgagggcg aggtagccgc cgacttcctg
      121 gagcggttgc tggacatcgc cgacctcgac ggtgacatcg aggtcgacat cgacggagac
      181 cgcgctgcgg tgtcgatcgt cgactccgag gaaggccgcg ttccgcgccg gctggtgggc
      241 cctgacggca aggttctgga tgccctccag gagctcaccc ggctcgccgt ccagaccgcg
      301 accggcgaac gcagccggtt gatgctcgac gtggccgggt tccgggccga ccgtcgcacc
      361 gcgctggtca ccctggccca agaggcgatc gccgaggtgc gcgcctcggg tgtggcccgg
      421 gcgctggaca acatgtcggc cttcgagcgc aaggtcgtcc acgacgaggt gacggccgcc
      481 ggcctggtct cggaatccga aggggtcgag ccgcaccggc acatcgtcat cagcccggcg
      541 tgaccgaggt tgatgtcccg gtcccgccgc tgtcggcggc acaggtcttc ggcccccgtg
      601 aagagctggc cagggcctat gtcggtctgc tcgcggacac cgggatcagc cacgggctga
      661 tcggcccacg cgaggtccct cgcctctggg accgacacgt gctcaattgc gccgtcatcc
      721 aggaactcct gcccccgaat gctctgtgcg ccgacatcgg ctcgggggcg gggctgcctg
      781 gtctggcgtt ggcgatcgct cggccggaca tcacgatgca tctggtcgag cccctgcagc
      841 ggcgggcccg gtggcttgac gtggctgtcg ctgagctggg tctgtcgggc gtgacggtgc
      901 attgctcccg ggccgacgcg ttgcacggcg tgctcaccgt ggatgtcgtc acagcccgag
      961 ccgtggccga gttggcgacc ctgggtcagt ggtgtctgcc cctactgccg cccggtggac
     1021 gtctgctggc catcaaaggg tccaaggctg aggccgagct cgccgaggct tggccggccc
     1081 tgcagcgtct ggggtgccag gccggttcgg tccgtgagtg cggtgggtcg atgatcgatc
     1141 cgccgacccg ggtcgtggag gtgattgcgg ggtctgcgcc atcgtcgggc aaaggacggg
     1201 cccgcccacc cagcgcccgc gcgcaacaac gcaaggcgcg agccgccgca gcgagacggc
     1261 accgtggagg gttctcgccc accggtgccg atgaggcatc accgagcaac tgacgcccac
     1321 cagcaccgat ctcgcccact acgggcctgg cctgtggatg atggcgaaag ccgagtcggg
     1381 cgctgtggtt ggatgcaggg atggagcggc gcgcacggac cagtcgggga ctggggtggc
     1441 cttggggttc atcccctggc cggcgaagcc ctctggggtg gcccgaccac ggttcggacg
     1501 atgatctggg tgaccagcag ccgttgacca gcgactcgga cccgacggtc gatgtttcac
     1561 gtgaaacacc tgacccgttg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 ngacccggca gctgagagca gcagcctcgt ggacctccag gacgagagca ccccgctcgc
     1741 ggctgccatc gcccaagatg tgcgccgtcg gtccaccctc gtcggagccc ccctgccgcg
     1801 ccccccggcc acccgagttc tcaccgtcgc caaccagaag ggtggcgtgg gcaagaccac
     1861 ctcagcggtg aacgtcgcgg ccgcccttgc gcagtcgggg atgcgggttc tcgtcatcga
     1921 cctcgacccc caaggcaacg cgagcacggc attgggggtg gaccaccggg ccggcatcga
     1981 ctcgatctat gacgtcctcg tggacggcca accgatgagc gcggtcgtcg tcgaatcccc
     2041 caccgtgccg ggactcttct gcgccccgtc gacgatcgac ttggccggcg ccgagatcga
     2101 gctcgtctcg ctcgtcgccc gtgagggccg cctggcgacg gcgctcaatg cgcacctggc
     2161 cgacacggca tacgactatg tcttcatcga ctgtcccccg agtctcgggc tgttgaccgt
     2221 caacgccttc gttgccgcgc gcgaagtgtt catccccatc caatgcgagt actacgcgct
     2281 ggagggcttg tcccagttgc tgcgcaacgt cgagttgatc aggacgcatc tcaaccccac
     2341 gctcgccgtc tcgaccatcc tgttgacgat gtttgacggc cggacccggc tgtcggccca
     2401 ggtggccgaa gaggtgcgag agcacttccc cgagcagacc ctgcgcacga ccatcccccg
     2461 gtcggtccgg gtgtcggaag ctcccagctt cggcgagacc gtcatgacct atgacccgtt
     2521 gagcagtggg gcgctggcgt atctcgaagc cgccagtcag atcgcccagc gaggtgccga
     2581 atcgatgaac gagggagcac atccgtgaag gaacgagaga agcgacgcgc gttgggccgg
     2641 ggcctggggg cgctcatccc cacggccccc gcgggggagc ggcccgtcga cgtcttcttc
     2701 gggccggcgc cgtcagcaat caccgtcgac cttggcacga agcacgccgc ggccgacggc
     2761 gacccgagcg acctgactga ttccgccgag accaccgacc cgaccgacgc ggctgccgcg
     2821 tcgtcgtcga gcagtcccac ggacgccccc tcggcaggca ccggaccggc agacccggac
     2881 aacgacgccc tgcgaccggt ccctggggcc acgttcgccg aactccccgt cgagtcgatc
     2941 cggcccaacc cacgccagcc acgcaccgtc ttcgacgaag acgacatggc ggagttggtc
     3001 cactcggtga gtgagatcgg catccttcaa ccgatcgtcg tgcgccgagt cgacgatggt
     3061 gggcccctcg gctcgggaca cgacgctgct atcgcgacct atgagctggt catgggggag
     3121 cggcggtggc gagccgcgca ggcagccggc ctcgaacatg tgccggcgat catccgctcc
     3181 acccacgacg acgacctgct gcgcgatgcg ctcctggaga accttcaccg cagccaactc
     3241 aacccgttgg aagaggcggc ggcctatcgg caactcctgg atgacttcgg ctgcacccaa
     3301 gaggagttgg ccacccggat cgggcgtagc cgcccgcagg tgagcaacac cctgcggctg
     3361 ctcaaactgc caccgctggt ccagcgccgc ctcgcggccg gggtgttgag cgccggacat
     3421 gcccgcgccc tccttggcct caccgacgcc gcggccatgg agcgcctcgc ccagcggatc
     3481 gtcgccgaag gcctctcagt ccgctccgtc gaggagatcg tcgccgtcgg ggcggtggga
     3541 acggctccgg ccaaacccaa gtccccccga gccggtgggc ggcggccgca actcgacgac
     3601 atggccgcgt ctctgtccga ccacttggac acccgggtga cgatcgcctt aggacaacgc
     3661 aagggcaagg tcgtcgtgga gttcgcctcg gtggaggacc tcaaccggat cctggacgtg
     3721 atcggcgtcg accgacccaa gcgctgaccg gacatggccg cgacacccaa gcgccgcaag
     3781 ggcggtccgg agattgtgcc ggtgacggcc gatgacgtgc ccgccctcgt cgagccgtgt
     3841 cgtgactgcg ccttctggga gcgtggaccc ggcctcgtcg cggggacggc gagcacagtg
     3901 agcagggaca aggctgcgtg ggtgcgcaga gtcgaggccg agtggggcac cccggggtgg
     3961 ctggttcggg tggacggtca gccggctggt caccttctca tcgccccggc tcatctcgcg
     4021 ccccgctccc tcgccttccc gacctccccg gtcgccgacg atgcgattct cctgctcgcg
     4081 gtgcgggtcg atccccggta tgccggtcag ggcctggggc gccgactggt ccagaccgcc
     4141 gcgcgggaag tcctctccca cgacggccgg gcgatcgagg cctttgggac cacccggggc
     4201 gatcagtgcc tgaccccggt cgacttcctc accgcggtgg gcttctacgt cgtgcgggag
     4261 cacgcggcat accctcggtt gcggctggat ctgcgcaccg cactggcctg gcgcgaggac
     4321 gtcgagcatg cgctcgatcg gatcctcgct ccggttcggc gcctgggggc cggcaacccc
     4381 gtcggcaccg cgaacaacga agcgcccggc gctggtcggc cctgaggccg ccgacaccgg
     4441 gcgctgcgat gaggctcagc cgaggaactg ctccaactca cgcagcaaca gccgcttggg
     4501 cttggcgccg acgatggtct tgacgacctc gccgccgacg taaaggttca tcgtcgggat
     4561 gccggtgacg ccatagcgcg cagtcgtcgc cgggttgttg tcggtgtcga gcttgacgat
     4621 ctcgatcttg tcgccgtact cggcgtagat ctcttcgagg atcggggcga tctgcttgca
     4681 cggcccgcac caggcggccc agaagtcaac gaggacaggc ttcgtgttcg cgagcacgtc
     4741 ggtggcgaag gtggagtcgg tggtcgcggc aatggcgccc atgtgaggtc ctttcacggt
     4801 gtgcggccca cggactggta tgccgtgcgc ccggtctggg tctgggtctt ggtctggtcg
     4861 aaaactagcg cgggggtctg acagtcggtg ctggctcagt gcgccgcagc gactgactcg
     4921 gcgacatcgg acagagcctc gaggtagtgc tgggtgtcga gggcggccgc acagccactc
     4981 ccggcagcag tgatggcctg gcggtaggtg tggtcgacga ggtcgccgca ggcgaagacg
     5041 cccggcaggt tggtgcgagt cgatcggccc tcacagacga cgtagcctgc gtcgtccaga
     5101 tcgatgaccc cgcggatcaa ctcgttgcgg gggtcgtgac cgatggcgac gaacaggccg
     5161 gtgacttgca ggtcgctggt ggcaccggtg acggtgtcgg agagggtcac gtgggtgagt
     5221 ttcgggtcgc cgtgcagggc ggtgacggca gcgttccacg tgaaacggat cttgggattg
     5281 gccagcgcgc ggtcggccat gatcttcgac gcccgcaact ggtcgcgccg gtgaatgatc
     5341 gtcacgctcc gggcgaactt ggtgaggaag gtcgcttcct cgatcgcgga gtcaccaccg
     5401 ccgacgacgg cgatgtcctg gtcgcggaag aagaacccgt cgcaggtggc gcaccacgac
     5461 acaccgtggc cggacaagcg cttctcctcg ggcaggccca actcgcggta ggccgatccc
     5521 atcgccagaa tgacgctctt ggcgcgccac acccggcctt caccgtcggt caccgtcttg
     5581 atgtcgccag caaggtccat ggcaacgatg tcgtcggtga ccatctcagt gccgaacttc
     5641 tcggcctgcg cgcgcatggc gaacatcagg tcggggccct ggatgccgtc ctcgaagccg
     5701 gggaagttct cgacctcggt ggtgttcatc aaggcgccgc cggcggtgac cgagccctcg
     5761 aagagcacag ggtgaaggtt ggcgcgggcg gcatacaccg cggcggtgta gcccgcggga
     5821 ccggacccga cgatcacgac ctcgcgcaca cccgtgtcgg tcgcagtctc gacagcgtca
     5881 ctcaccggaa ctcctcgatc gtttgcggcg cttcaccgct gtgggctcgc gacacggcac
     5941 atgtggtgtc gtcgcggggc aggagaccca agatacccgc ccaatccccc caaccatcgc
     6001 ggcgcaccgc agattccccg acggttcacc tcaggggtat gccgtccgct caccgaccgc
     6061 cgtcgcggaa ccattggacg tcacgatcgc ccctcatcgt gtgccagcag gtcagggcat
     6121 gtcgacggga ccgaagagca gccccgggtc gcccttgctg caactgcgac taacggcata
     6181 cgcctgcttg agctcagcgt cgacgacgac gatgaccacc gcgggggcgc cctcgaacgt
     6241 ggccagatcg gcgaacaccg cgtcggcatc gctttcgccc agggtggtca cgcagtcggt
     6301 cagaccgatc agggtgccaa tgggccccac cgagggcgcc tcgacggcgg gttgccctaa
     6361 ctgccggccc ggcgcgtcga gcatgtcctg cgcgaggcgg gcgagcgtgg ccttcgtgta
     6421 ggtgcgggtg ctcaactcga tgtgcatcgg ctgcccgttc gcattcacgg ccgtcgccgc
     6481 gtttgccctc gtcgctgagt cggcaggagc actcagactg ctggagtcct tcgcggcccc
     6541 tacgaccgac tcggcacgcg cgctcgacgc agtaggcgat gtggcgccct ggttgttgag
     6601 gagggcgccg actaggccca aggcagcaac ggaggcggca acacccccga ggacggtcaa
     6661 ccgccgacga tgatcgacga cccgcccgcg catacctgcg cccgagcgcg accccgagcg
     6721 cgaccccgtg cgcacgtcgg tccgagcacc cgacgtctca gatgatgttc ccgctggctg
     6781 cgtcccacgc ccgacgaggt gcaaaggcac cgccggcccg gactcgtcaa ctcccgaaac
     6841 cccttggccc gcaacaagat tcgcaaagtc gtcaaacgcg tcgacaccca ggccgagttc
     6901 gtcgctcaac tcttgcgagc cagtgtcggc atcgaggtcg gcgcgtcgac cggcctcggc
     6961 gaaggcagca agaatccggt cggacacgtc cgccggcatc ggccccggat cgggctggga
     7021 ggcaagcagg atccgcaccc cggtcggatc agggtcctcg tcgaacctgg tcggctcgct
     7081 catcaacact ccctcgggat gtggctcgtc tggcccgccc cccgacggac cggcccgcag
     7141 ctgggcatgg gtttcaggac tgagacgcac gccgggacgc gctggttccc gcggcatacc
     7201 gtggcggtca tcggcgaatc cccagcaact gcgccatcgc ggcccgcccg cgggcgcacc
     7261 gtgacttgat ggtcccctcg gccacgccga ggatctcggc ggtctcggcg acggacaggc
     7321 catgcatgtc gaccagcacg agcgcggccc gctgaccctc cggcagttgg tcgagggcgg
     7381 cgcgcacgtc caggcgcacc tcgacggcag agtgggcgtc gctgcgatcg gccaggtcgt
     7441 agtcgggcag cggactcgtg ggccgggtcc ggcgcagccg gtcgaggcag gcgttgacaa
     7501 cgatccgatg cagccacgtg gtcactgcgg actccccgcg gaaggacccc gctctccggt
     7561 atgccgcgat gaaggcgtcc tggacgcagt ccgaagcgag ctcggggtca ccgcaggtgc
     7621 gcacggcgac cgcccacatc cggtccttgt ggcgtctgaa gatctcggcg aaggcctcgc
     7681 ggtcgcccgc gcagtgtgcg gccagcaact cggcgtcgga gcgcccggcc aactccactc
     7741 gacccgaggt caccggctga ccgccacttc ggacacctgg gcccggaagt tgcccgcgcc
     7801 gtcgggtcca agcttggtga cgaagacgat gacgagttgg ccgctggaca cgggcccggc
     7861 cggggcatcg aagacgagtt ggccgctctg gccggtgctg gagccgatcg ccgtggcccc
     7921 gtcgagcgag gcccggttgg cgacgtagac cgtgatgtct tgcggggcag gcagcgtgac
     7981 ggtcacccgg cggacctccg tctgctggcc gaggtcgacg atgtagccga tcccgggcac
     8041 cttgaggccg ccgaacttct cgttggaata ccacttcgag cgccacatcg tcgcggcgtt
     8101 cccgtcgtaa cccctcggtg cgctgctcga tgccacctgc ccggcgctcg catcgaaagt
     8161 ggcggccccg atgatggcca cgggggtcag cgaagcaaca accgcagggc cgtcgccggc
     8221 cgtggccccg gctccggtcg cgcccgcgct cgacgtgacg gtcgccgtcc tcgtgaccct
     8281 cgcggtcgtg accgacccgc cggtcgtccc acccgacgtc agcttgggca gcccccagat
     8341 ccccagcacc gccaggagca cgaccagacc ggcgacgatc gccagcgcga ggcgggactc
     8401 gtcgcgcccc aactcgccgg tgatcggaac gagcgggacc gcatcctcga ggggactgtc
     8461 caccggagcg aggaccgaag ccagcgtcgc gtcctcgcgg atcaggtcag gggcccgcag
     8521 cgtccgcggc gcgaccaccc tcggcgctcc aggcgccacg cggggcgccg agggcccagg
     8581 tgccgtcggc gtgccgctgc cgggcgagcc gggaggctgc gcggacgagg cttcgggcgc
     8641 tcccgctcct ggtgcggcgg gggcggacgc gatcccctca tcggtggccg gttcgggtcg
     8701 ggttgagagc cgttcgacgg tatgccgtgc ccgcccaccc aggcgtgacg cggcctgaga
     8761 cgctgcggtg ccagcggcac cgagggccga ccccacgccc cgggctccct tggccatagc
     8821 ggcactgacc gcggcagcag tcgccgcggc actcgtggca cctgcgcctc cggcggctcc
     8881 gccagccccg gtggccaacg agacgccctc ggccgtggcg ccaccgccag cactggacga
     8941 cggttgctgc tgcgacgaaa atggctgcgc tgcagggcta tcgggctgaa cgaggctggg
     9001 cgctgctgga cccgcgcctg cagctgtgcc tccacccgca actgcaccct gcccggcacg
     9061 ggggcctgca ccctggcctt cggtggcgct gggaaccgcc ggtgggcgcc ggtcacccca
     9121 gagatcgacc gtcggcccct tgggccaagg tcgcagacca gccaagacat cagcggacgt
     9181 gctgggcccg gcgtgctcga cgaaggtcgc tcgggccaac cgctgaaggt cgctgcacgc
     9241 gtcggctcca agctcgtcgg cagccacgac cgtgccgccg acaacgggcg cccgcgggag
     9301 cccggagtcg gcaccgccga gcggccagcg cccggtgagg gcggcatacc cgatgctcac
     9361 caaggcggtc gcgtccaggc gcgctccgac gtcggcggga gtgtcttcca ggccgagcag
     9421 cgccgcctcg acagcgactc cgcggacccg gaccgagccg tcgggcagca ggagcacgtt
     9481 gcgcggagtg aggacgatgt ggcgcaggcc gcgggcggcg gcggcatcca acgccatggc
     9541 ggcctcgccg gtgatgcgtc gggcctcgtc ggcgggcagg ccgccctgtt caaggatccg
     9601 ggtgaggctg cgggagcgct cgaccggctc ctcgatgatc gcgcagacaa tggcggcgct
     9661 cgccgggcct cccgcggagc ctcctgccgc ctcactcggg ctaccgctcg ggctactcgg
     9721 aacctgagcg tcggtggtcg gcggggtgac gatgtcgagc acccgcacga gccgaggctc
     9781 ggtcaccgcg gcggcccgcc tggccgcatc gacagcggct gcggcggctg gggtgccccc
     9841 cgcgaaggtg aggatgacga catcgcgacc gagcgtggtg tcgtgtgcgg cccagcgctc
     9901 ccaccggggg tgctgctgga ggcggtgcga cacggcatac cgatcgccga tcagggtgcc
     9961 gggaccgacg tgctgcatgg tcgctccctc cttgacgggg gtttggcccc cacctcactg
    10021 cccgctcggc tcatcctatg gtgcggtatg ccgttggacc ggcgcgacaa cgggcgcttc
    10081 gggggtgggg tcaacgggat ccagaccacg ggtatcgacg accatccgcg gcgggcggcg
    10141 gacggctcga acgatgccgg cgatgaggat gaggccgaac aggacgctca tgccgatgta
    10201 gagccaggcc ccgggtgggg ccgcgcggat ggtcagttgt tggacggcgc cgatccgggt
    10261 gccgtcggct gtcgtcagcc aggcgttgac cgggaccaga ccttgggcca gcacggccag
    10321 gcgcacggac acggtcgcct tggcattggc cgcgatgtcg acgggtgcgc cggggtcgac
    10381 gacgacgatc cggccattgc caggtgcgag cacggcgcgc aggccgcgca ccggctcgtc
    10441 gagggagttg acgaccgtga gctgcatgac gccttcgtcc gcaaggaaat tcgtcgtctg
    10501 tgggatgacc atgagcccgt tcgacacggt cgcggtcgac tgctcgagcc cggccagcag
    10561 cgcctcgcgg ccggcgggat cggtgcgcca gcgcgtgctc gccgcctgtc ccggcagatc
    10621 cgaccaggcg gcgatcgtgg ggctgccctg cgccaggacg ctggagagct ggtcgatgac
    10681 ggagcgctgc tggaacactc gggcgaggag ggaacggccg gccgccgggc cgttggacgc
    10741 agcggcactg gccgggtcgg tgggggtgac ccgcgaagct ggcgcatcgg tcggcaaggg
    10801 aggagcagaa tccaggcctc gaccggggag catcgcggag tccaccccga cggtgcgcag
    10861 ccacggcacc tgcttggtgg cgtcgaagaa cgtggacacc aggtcggcgt cgagcccggc
    10921 gtcgaagccg cgcggtggca cggcgagaat ggtgcgagag atcgacggcc gctcagcgag
    10981 cagcgccgcg gtttgcgcca cgaactcctg ggcggccaac accgcctcgc gcggggtctt
    11041 gaggcgggcc agcaaggcgc cgagccggtc gtcccagcgc agcacggtcg tcccgagggg
    11101 ggcgaccccc agcgcgggcg gggtcagtcg cgcagacggg tccgtcacct gggtcgacac
    11161 gaggatggca tctaggcggg atgcgtatgc cgcccgcagc gccgcctcac gtccgctcgg
    11221 cagggccccg tcggccggcc aggcgagcgt cccgcgggtg gtcaccgcgg catcagtcag
    11281 ccgggtggac tggccgaccg cctcggccaa cagttgggag gccgctgggg acagggtggg
    11341 gccgaggtcg ggtgcggtga gagcagccag gtccgggtcg ttctgcggca acgcgaagac
    11401 ggggtgcgtg gcggaggcgg cgctcaacga catcgagaac gccgagcgca ccgcacttgc
    11461 cgggtcggcc gcgtcctggg ccggggtccg acccgacggt ccgagcacgg cggggtccac
    11521 ggcatacccg acggtctgtg aagcggtcgc gcgcaagaga gccgccaggc gcgagccggc
    11581 tccgacctgc ttggtccagg cggccgttcg cgcggtgggg tcgtcggcgg tgagggcggt
    11641 gtcgggggcg agcgtcaccg gcaacagcca ggcgagggag agcgcttcgt agtcggccga
    11701 tcgttgccag ccgacgaagg tgcgtaacgc cgagacgctc gtgcccttgg ccgtgaccac
    11761 gtcgacggcg atcgggctga cgccataggc gcggccgggt cggacctgat cggcggggac
    11821 ggtgaactgg aacgggatgg cgttgcgcgg tgcggccggg tcgaggggct ggcgaccgag
    11881 ctcggtcccc gagaccgcac cggtggcggc cgaccactcg tcaacatcgg ctcgggacgt
    11941 caggcttgag cgaccgcgga ctaggcgcac cgtcgtcggg atcccgtcgg ccgcggtgct
    12001 cgtcacgtcg atcgtccccg tcaccaggag ggtgtctccg gggcgcaaca cggcggggga
    12061 gacggccgtg agggtgatcc ggtcaccgga cggatcggct cgggggatcg tgaccgtcgc
    12121 ggtcgcagta gttgcagtgg cagtagttgc agtggcaggg gtcgcag
//
LOCUS       DAORPW010000067        11947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1146_length_11947_cov_713.747057, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000067 DAORPW010000000
VERSION     DAORPW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11947
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1146_length_11947_cov_713.747057"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1073
                     /locus_tag="PLB86_07065"
     CDS             <1..>1073
                     /locus_tag="PLB86_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRC64934.1"
                     /translation="RAEVGDSNSLTVAAGDLIGGSPFLSGMFHDEPSVESLETLGLDV
                     SSVGNHEFDEGSAELLRMQRGGCHPVDGCYFPDQPYDGASFTWLAANVVKKSNGKPLL
                     PPVAVRAVKGVKIGFIGMTLEATPTLVNPAGVASVDFKDEIETANAQAAALKKRGVKT
                     IVVLLHEGGVNPSGISSCAGISGPILAIAQGITHEVDAIITGHTHEPYICQIPDAGGV
                     PRLVTSAASYGQVVTETTLVVDKATGEVDRSASVAENHLVLRSIGKDPAETAVIAKWN
                     TLAAPMAARIVGSHVEDILGDSNGNRGIETPMADLVADAWLYGTASAANGGAQIAFQN
                     VGGTRASFRVNSTPNGEQPGQIT"
     assembly_gap    1074..1173
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1174..2252)
                     /locus_tag="PLB86_07070"
     CDS             complement(<1174..2252)
                     /locus_tag="PLB86_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HRC64935.1"
                     /translation="MTSEQQVKTDNRPRPTTEQFRAFVREGWAPRSTELPARAEVAPY
                     AAARREALSAAYPGERLVIPAGGLKVRSNDTDYVFRPHTAFAHLTGFGGDREPDAVLV
                     LHPRSEADGGGHDAVLYFRPMAGRDTEEFFADARYGEFWVGARPTLADIEAEFGLVAR
                     HIDDLPDALAKDAGAVTLRVVRDADSDVAALVDQARTEAGVEVTEEITEADVALAAYL
                     SQLRMTKDEWEIQQMRDAVAATKVGFEAVIADLRSAVERGRGERWVEGLFGLHARHQG
                     NGVGYDSICAAGDHANTLHWIKNTGEINEGELLLLDAGVEVDSLYTADITRTLPVDGT
                     FTDAQREIYDAVYAAQEAAMAAIRPG"
     gene            complement(2299..2778)
                     /locus_tag="PLB86_07075"
     CDS             complement(2299..2778)
                     /locus_tag="PLB86_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64936.1"
                     /translation="MTRLRIAAALALAAGLTLLGAGGQGQAHADEVTVTITPTVVIVT
                     DATGRPIRTETIIPTPPAATPADQPGPKTVTVTVAPPNPGVLTLGQNDAGQWIGMVIG
                     AVLGALGTVAVMTRRRRETPVGSDESAPEPEEDGSPEPDVSPDLAAEDLSPPPDPAT"
     gene            complement(2775..4148)
                     /locus_tag="PLB86_07080"
     CDS             complement(2775..4148)
                     /locus_tag="PLB86_07080"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRC64937.1"
                     /translation="MIHRVGRAWLAPGAAFVLALVLRMVDLGRVKALVFDEAYYVPQA
                     WSLLNYGIERKFAKDGQSVFVSGDFAGAFTSGGDFTVHPPLGKWLIALGETAGASQPW
                     TWRISAAVAGALIAALVAAMAIHLTRSSWWGGFGGVLLAVEGTSLVEGRTGILDTFLT
                     LFVVAGAAAALMDRDDPTPGRFRRWRLAAAVCLGLATGVKFSGAVFLVVFILLAIAWD
                     VRRRPLSAARSIGVAARTLVTISVPAAAAYTLSWAGWFLSPDGHGRDGSDGLLMNWIH
                     NQTDMLALGGAISSAHHWQSGPWAWLIQWRPTMFYVVTAPCEGTTGTCSQTITSLGTV
                     PIWWVGIVALVGLGWLLARHRDRDAAFILAFFAAGYLPWWFLTNRTIYAFYTVTIAPF
                     VILAIVLCLYRLQRSQAHRRVLAQPAALLLLAAAIAWLWLYWPVYIGIPIPQGESARL
                     LFWEGWQ"
     gene            complement(4166..5158)
                     /locus_tag="PLB86_07085"
     CDS             complement(4166..5158)
                     /locus_tag="PLB86_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64938.1"
                     /translation="MRIRHLVGAAALSLAATMATLPNAAGAPLPAPIVTQTFSSTETL
                     TATTVAGPVPQTVTHTQTMTTSGEVRTTEWVTVTTTSTAWLDATQTQTTTVDANAEPV
                     IMTATALFTAPGVTVTVPGVPVVTAETRTATDILIGKPTETQTIAVACGTVPTVVVTP
                     TSVHTTTITPTQTAVTSTTTLKDLRSTVTETRSTTSVYTELATGDTAWETVTVSNEEG
                     GAWTMTTIITTTQTPTTTVTDVPVTLTNTVYDQVTDTVHPIGYVAVETGCPTTTTTTP
                     SAETPTTTAPLLANTGSNSAPALVLGLLFILGGSAILALVYRRQPSSHRGSTRR"
     gene            5339..6370
                     /locus_tag="PLB86_07090"
     CDS             5339..6370
                     /locus_tag="PLB86_07090"
                     /inference="COORDINATES: protein motif:HMM:NF021599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HRC64939.1"
                     /translation="MVTFDAGETLAERFRSHMGHSSSLYGYAMRGMADDWEAGGPTRE
                     ACRGWEDSPNGTMVQLRFLAGVFRLVLAGAAPELVPYYPCLGGTLPPDGVWPVMRQVV
                     GRHTEQVHEALAIVPQTNEPGRSTALLVGLFDAVEAWGLDRIRLIEVGASGGLNLHVD
                     RFRYDGPGWSWGPPDSPLVLHTQAPGVTPRAFEIVERLGCDVAPVDAASHEGALHLRS
                     FVWPWQLERHERLTGALAVAAAYPVVVERAGAGEFLQCVLADPPADGVLTVVWQSITR
                     MYWPATESARVAALIEDARSRMPLAHVSMEHADTPDAADAELAVDGRVLGVVHPHGIP
                     LWVRPGTHA"
     gene            complement(6390..7445)
                     /locus_tag="PLB86_07095"
     CDS             complement(6390..7445)
                     /locus_tag="PLB86_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRC64940.1"
                     /translation="MKTATGNFFEDFTLGMRLVHATPRTVSDGDVALYTALYGPRFAP
                     TSGVTMARAMGLERMPLDSMLVFHVVFGKTVPDVSLNAVANLGYAAGRFGVPVYPGDT
                     ITAESEVIGLKANRDGKTGVVYVHSVGTNQDGATVLDYVRWVMVRKRDESAPAPETVL
                     PELPSAVPVGDLDVPWDLRGGHYDTALGGSPYLWDDYAVGERIDHVDGMTIEESEHML
                     ATRLYHNTAKVHFNQHVESQGRFGRRIVYGGHIISIARALSYNGLANAVSIAAINGGT
                     HSNPTFAGDTVYAWSEVLERLELPGRTDMGALRLRTVATKDLACGEFPYRGDDGKYLP
                     NVVLDVDYTVLIPRRQG"
     gene            complement(7559..8410)
                     /locus_tag="PLB86_07100"
     CDS             complement(7559..8410)
                     /locus_tag="PLB86_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC64941.1"
                     /translation="MRHGLTILPEHPWREAAPMWRAAEDFGFDHAWTYDHLVWSGLPD
                     QTWFAAVPTLAAAATVTERIGLGTFVSSPNYRHPYTYARDILTLHDLTEGRFICGVGT
                     GGTLDSGILGETLTVRERVDRFAEYLAVLDRLLRADHVDHSGPWFTTVDARTLPGPVR
                     SSVPLVVAANGPRALRLAAAYGEGWVTTGTGGDDLDAWFQGVAALTARLDDALAATGR
                     AEGFARYLSLDASPRFALESADLYAEMVGRAGELGFTDVITHWPRPDGPYAGDVRVLE
                     TVAATLG"
     gene            complement(8595..10301)
                     /locus_tag="PLB86_07105"
     CDS             complement(8595..10301)
                     /locus_tag="PLB86_07105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HRC64942.1"
                     /translation="MSTPTALSPAYRSAAIDRLASGEELDILVIGGGVTGAGCALDSV
                     TRGLRTGLIEKRDFASGTSSRSSKLIHGGLRYLEMFDFRLVAEALKERGLLLTRLAPH
                     LVRPVPFLYPLTKPVVERPYVEAGLALYDSMARASRHSAGLPWHKHLTRRQALKIAPA
                     LKSTALVGAVQYYDAKVDDARHTMELARTAASYGAHVATRVAAVGFLRQGERVTGVRA
                     LDKVTGREFDILARQVVNATGVWTDDTQALVGERGQFHVRASKGVHLVVPRDRIHSKS
                     GIILRTEKSVLFVIPWGRHWIIGTTDTDWALDKAHPAATSADIDYLLGHVNRILRTEL
                     TQADVEGVYAGLRPLLAGESDQTSKLSREHVVAHPTPGLVVVAGGKYTTYRVMAIDAI
                     DEAARGLGGEVPPSTTEDIPLVGAVGYRAMWNRRARLAHESGLHVERIEKMLMRHGVL
                     TTEVLALVAADRSLGEPLPGGEDYLKAEIVYAASHEGALHLDDILGRRTRLSIESFDR
                     AITASRPAAELVAPILGWDAATVDEEVEHYRQRVEAERMSQTMPDDASADAARLQAPD
                     RG"
     gene            complement(10354..11883)
                     /gene="glpK"
                     /locus_tag="PLB86_07110"
     CDS             complement(10354..11883)
                     /gene="glpK"
                     /locus_tag="PLB86_07110"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRC64943.1"
                     /translation="MADFVGALDQGTTSTRFMIFDHAGREVGKHQLEHEQILPRAGWV
                     EHNPLEIWERTQAVIGSALTTAGLHASDLAAMGVTNQRETTIVWDRHTGRPWHHAIVW
                     QDTRTDAIAAALDADERGEVIRERAGLPPATYFSGGKLQWLLEHVDGLRAAAERGDAL
                     FGTPDTWVIWNLTGGPHGGRHVTDVTNASRTMLMDLRTLDWDDELLGFFNVPRAMLPT
                     ILPSGDADRYGVTATSGGSNAWGGPIKIAGDLGDQQAATVGQVCFAPGEAKNTYGTGN
                     FMLLNTGTEPVRSKAGLLTTVAYQFAGEQAAYALEGSIAVTGSAVQWLRDQLGIISGA
                     ADIERLAAQVPDNGGVYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLEAI
                     CYQTKDVADAMFADSGVRLDTLKVDGGVTANALCMQIQADILGVQVSKPVVAETTALG
                     AAYAAGLAVGFWRTTDELRANWQEDRRWSPQWTDEQRASGYAAWRKAVSRTLDWAPVS
                     G"
BASE COUNT         1754 a         3930 c         4322 g         1841 t
ORIGIN      
        1 cgggccgagg tcggcgactc caacagcctc accgtcgctg cgggcgacct catcggtggg
       61 tcgccgttcc tctccggaat gttccacgac gagccgtcgg tggaatccct ggagaccctt
      121 gggctggacg tcagcagcgt cggcaaccac gagttcgacg aaggctcggc tgaattgctg
      181 cgcatgcagc gcggcggctg ccacccggtg gacgggtgct acttcccgga ccagccctat
      241 gacggggcgt cgttcacgtg gctcgcggcc aacgttgtga agaagtccaa tggcaagccg
      301 ttgctgcccc ctgtggcggt gcgcgcggtc aagggtgtca agatcggctt catcgggatg
      361 accctcgagg ccacgccgac attggtcaac ccggcaggcg tggcgtcggt tgacttcaag
      421 gacgagatcg agacggccaa cgcccaggcg gccgcgctga agaagcgcgg cgtcaaaacc
      481 atcgtcgtcc tcctgcacga aggcggcgtc aacccgagtg gcatcagctc gtgcgccggc
      541 atctcgggcc cgatcctggc tatcgcccag ggcatcaccc acgaggtcga cgcgatcatc
      601 accgggcaca cccacgagcc ctacatctgc cagatccccg acgccggcgg tgtcccgcgg
      661 ctggtcacca gcgcggcctc ctacggccag gtcgtcaccg agacgaccct cgtcgttgac
      721 aaggccaccg gtgaggtcga ccggtccgcg tcggtggcgg agaaccacct cgtgctgcgc
      781 tcgatcggca aggacccggc ggaaaccgcg gtcatcgcca agtggaacac cctggccgcg
      841 ccgatggcgg cccggatcgt cggctcgcac gtcgaggaca tcctgggcga ctccaacggc
      901 aaccgaggca tcgagacgcc gatggccgat ctggtcgccg acgcctggct ctacggcact
      961 gcctcggcgg ccaacggcgg cgcgcagatc gccttccaga acgtcggggg caccagggcg
     1021 agcttccggg tcaactcgac cccgaacggc gagcaaccgg ggcagatcac ctannnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccgggc ggatcgccgc catggccgct
     1201 tcctgcgcgg catacacggc gtcatagatc tcgcgttgcg cgtcggtgaa ggtgccgtcg
     1261 acgggcaggg tgcgagtgat gtcggcggtg tagaggctgt cgacctcgac cccggcatcg
     1321 agcaggagga gctctccctc gttgatctcg ccggtgttct tgatccagtg cagggtgttg
     1381 gcgtggtcgc cggccgcgca gatcgagtcg tagccgaccc cgttgccctg gtggcgggcg
     1441 tgcagcccga agaggccctc gacccagcgc tcgccgcgcc cgcgctcgac cgccgagcgc
     1501 aggtcggcaa tgaccgcctc gaagcccacc ttcgtcgccg cgaccgcgtc gcgcatctgc
     1561 tggatctccc actcgtcctt ggtcattcgc aattgcgaga ggtatgccgc gagcgccacg
     1621 tccgcctcgg tgatctcctc ggtcacctcg acaccggcct cggtgcgagc ctggtcgacg
     1681 agggccgcga cgtcactgtc ggcgtcacgg acgacccgca gggtgacggc gccggcgtcc
     1741 ttggccaacg cgtcgggcag gtcgtcgatg tggcgtgcca cgagcccgaa ctcggcttcg
     1801 atgtcggcca gggtcggccg cgcaccaacc cagaactcgc cgtaacgggc gtcggcgaag
     1861 aactcctcgg tgtcgcggcc ggccatcgga cggaagtaga ggacggcgtc gtgcccgccg
     1921 ccgtcggcct cactgcgggg gtgcagcacc aggacggcat ctggctcgcg gtcgcccccg
     1981 aagccggtga ggtgggcgaa cgcggtgtgc ggccggaaga cgtagtcggt gtcgttcgac
     2041 cggaccttca ggccgccggc ggggatgacc agccgttccc cggggtatgc cgcgctgagg
     2101 gcctcacggc gcgccgcggc atacggcgcc acctcggccc gcgcggggag ctcggtggag
     2161 cgtggtgccc acccctcgcg cacgaaggcg cggaactgct cggtggtcgg ccgcgggcgg
     2221 ttgtcggtct tgacctgctg ttcactcgtc acccgaccaa tgctcgcatg tcggtgccga
     2281 atgggaacgc cggtcgggtc aggttgccgg gtccggcggg ggcgagaggt cttcggcggc
     2341 gaggtcgggg ctgacgtcgg gctcgggtga cccgtcctcc tcgggctcgg gcgcgctctc
     2401 gtccgatccg acgggcgtct cccgacgtcg gcgggtcatc accgcgacgg tgccgagcgc
     2461 gcccagcacg gcgccgatga ccatgccgat ccattgccca gcgtcgttct ggcccagggt
     2521 caacacgcct gggttgggtg gggcgacggt gacggtcacc gtcttgggcc caggctggtc
     2581 ggcgggagtc gcggccggcg gcgtagggat gatggtctcg gtgcggatgg gccgcccggt
     2641 ggcgtcggtg acaatgacga cggtcggggt gatcgtcacg gtgacctcgt cggcgtgggc
     2701 ctgaccctgc ccgcctgcgc ccagcagggt caggccagcg gcgagggcga gggcggccgc
     2761 gatgcgcagg cgcgtcattg ccagccctcc cagaacagca ggcgggccga ctcgccctga
     2821 gggatcggga tcccgatgta gacgggccag tagagccaca gccaggcgat cgcggcggcc
     2881 agcaggagca gggcggccgg ttgggcgagc acgcgccgat gggcctggct gcgctggagt
     2941 cgatagaggc acaggacgat ggcaaggatg acgaacgggg cgatggtcac cgtgtagaac
     3001 gcgtagatcg tgcggttggt gaggaaccac caggggagat atccagcggc gaagaacgcc
     3061 aggatgaagg ccgcgtcacg atcgcggtgg cgggccagca gccaccccag cccgacgagg
     3121 gccacgatgc cgacccacca gatcggcacc gttcccagcg aggtgatggt ctggctgcag
     3181 gtccctgtcg tgccctcgca gggagcggtg acgacgtaga acatcgtcgg gcgccactgg
     3241 atcagccagg cccaggggcc ggactgccag tggtgagcag agctgatcgc accgccgagg
     3301 gcgagcatgt cggtctggtt gtggatccag ttcatcagca ggccgtcgct gccgtcgcga
     3361 ccgtggccat cgggggagag gaaccagccc gcccaagaca gggtgtatgc cgccgcggcc
     3421 gggacgctga tggtcaccag ggtgcgggcg gcaacgccga tcgatctcgc cgcactgagg
     3481 ggacgccggc gcacgtccca ggcgatcgcc aacaggatga acaccacgag gaagaccgct
     3541 ccggagaact tcactccggt cgccagcccc aggcatacgg cagcggcgag tcgccagcgt
     3601 cggaaccggc caggggtggg gtcgtcgcgg tccatcagcg ccgcggccgc tcccgcaacg
     3661 acgaagaggg tgaggaaggt gtcgaggatg ccggtgcgac cctcgacgag cgaggtgccc
     3721 tcgacggcga gcagaacgcc gccgaaccca ccccaccacg aggatcgggt gaggtggatg
     3781 gccatcgcgg cgacgagcgc tgcgatgagc gcccctgcca ctgcggccga gatccgccac
     3841 gtccagggtt ggcttgctcc cgcggtttcc ccgagggcga tgagccattt gccgaggggc
     3901 ggatggacgg tgaagtcacc gccggaggtg aaggctcccg cgaagtcgcc gctcacgaag
     3961 accgactgcc cgtccttggc gaacttgcgc tcgatgccgt agttcagcag cgaccaggcc
     4021 tgcggaacgt agtaggcctc gtcgaacacc agggccttca cccgcccgag atcgaccatc
     4081 cgcaggacga gcgccaacac gaaggccgcg cccggcgcga gccaggcgcg gccaacgcgg
     4141 tggatcatca cggcaccaac gtagatcagc ggcgcgtcga gcctcggtgg gaggacggct
     4201 ggcggcggta gaccagggcc aggatcgccg agccgcccag gatgaacagc aagcccagca
     4261 cgagcgccgg agcggagttc gagccggtgt tggccaggag tggggcggtc gtcgtcggag
     4321 tctcggcgct gggcgtcgtc gtggttgtcg tcgggcagcc ggtctcgacc gccacgtagc
     4381 cgatcggatg caccgtgtcg gtcacctggt cgtagacggt gttcgtcaac gtcacgggta
     4441 cgtcggtcac cgtcgtggtc ggagtctgag tcgtcgtgat gatcgtggtc atcgtccagg
     4501 cgcccccctc ctcgttcgag accgtcaccg tctcccacgc ggtgtcgcca gtggccagtt
     4561 cggtgtaaac ggacgtcgtg gaccgagtct ccgtgaccgt cgaccgcagg tccttcagcg
     4621 tggtcgtcga ggtgacggcc gtctgcgtcg gcgtgatggt cgtcgtgtgc acggacgtcg
     4681 gggtgaccac gacggtgggc actgtgccgc aggccacggc gatcgtctgg gtctcggtgg
     4741 gcttgccgat caggatgtcg gtggcggtcc gggtctcggc ggtgacaact ggcacacccg
     4801 gcacggtgac ggtcacgcct ggtgccgtga agagggccgt tgcggtcatg atgacgggtt
     4861 cggcgttggc atcgacggtc gtggtctgag tctgcgtggc gtcgagccat gccgtgctgg
     4921 tcgtcgtcac cgtgacccac tcggtcgtgc gcacctcgcc cgacgtggtc atcgtctggg
     4981 tgtgggtcac cgtctgaggg accggcccgg ctacggtcgt cgccgtcaaa gtctcggtgc
     5041 tggagaaggt ctgggtcacg atcggcgcgg ggagtggagc acccgccgcg ttgggcaggg
     5101 tcgccatcgt cgccgctagg gacagcgctg ctgctcccac caagtgtcgt atgcgcatgg
     5161 gtgcactccc gtgtcgtgta ctcccagtcc gcccgcgacc ccccgcgtgc ggactcttct
     5221 cccgtgagcg gatcgtaccg cacatcgact aacccatgtt aaggaagtct ctccaggacc
     5281 tgttcggagg tcggtggagg cgaagtcccg ggtgacgagc ccgtgacatg ctgacgtgat
     5341 ggtgaccttc gacgccgggg agacgctcgc cgaacggttc cgttcccaca tgggtcactc
     5401 gtcgagcctc tacgggtatg cgatgcgggg catggccgac gattgggagg caggcggccc
     5461 cacacgtgag gcctgtcgcg gctgggagga ctccccgaac ggcacgatgg tgcaactgcg
     5521 cttcctggcc ggcgtcttcc gcctggtcct ggccggtgca gccccggagt tggtgcccta
     5581 ctacccgtgc ctgggcggga ccctgccgcc cgacggggtc tggccggtca tgcgtcaggt
     5641 ggtggggcgg cataccgagc aggtccacga ggcgctggcg atcgtcccgc agaccaacga
     5701 gccgggccgt tcgactgccc tgctcgtcgg cctcttcgac gcggtggagg cctgggggct
     5761 cgatcggatc cggctcattg aagtgggggc gtccggtggg ctcaacctgc acgtcgaccg
     5821 gttccgctac gacgggccgg ggtggtcgtg gggaccgccc gactctccgc tggtgctgca
     5881 cacccaggcg cccggagtca ccccccgagc gttcgagatc gtcgagcgcc tggggtgcga
     5941 cgtggccccg gtcgacgctg cctcgcacga gggagcgctg catctgcggt ccttcgtctg
     6001 gccgtggcaa ctcgagcgcc acgagcgact caccggcgcg ctcgcggtgg cggcggcata
     6061 cccggtggtg gtcgaacggg caggagcggg ggagttcctg cagtgcgtgc tcgccgatcc
     6121 acctgctgac ggggtgttga ccgtcgtctg gcagtcgatc acccgaatgt attggcccgc
     6181 aacagaatcc gcgcgggtcg ccgcactcat cgaggacgcc cgatcgcgta tgccgctcgc
     6241 gcacgtgagc atggaacacg ccgacacccc cgacgcggcc gatgccgagt tggccgtcga
     6301 cgggcgggtg ctcggggtgg tgcacccgca cggcataccc ctgtgggtgc gtccggggac
     6361 ccacgcgtga cgcgcgggca aactgcgggt cagccctggc ggcgcgggat gaggaccgtg
     6421 tagtcgacgt ccaacacgac gttggggagg tacttcccgt cgtcgccgcg gtaggggaac
     6481 tcaccgcagg cgaggtcctt ggtggcgacc gtccgcaacc gcagcgcgcc catgtcggtg
     6541 cgccccggaa gttccagtcg ctccagcacc tccgaccagg cgtagacggt gtcaccggcg
     6601 aaggtcgggt tgctgtgcgt gccgccgttg atcgctgcga tcgacaccgc gttggccagt
     6661 ccgttgtagg acagtgcacg cgcgatgctg atgatgtggc cgccatagac gatccgccgg
     6721 ccgaaccggc cctgcgactc gacgtgttgg ttgaagtgca ccttggcggt gttgtggtag
     6781 agccgggtcg cgagcatgtg ctcggactcc tcgatggtca tgccgtcgac gtgatcgatc
     6841 cgctcgccga ccgcatagtc atcccagagg taggggctgc cgccgagcgc cgtgtcgtag
     6901 tggccgcccc gcaggtccca ggggacgtcg aggtcgccga ccgggaccgc gctcggcagt
     6961 tcaggcagga cggtctcggg agccggcgcc gactcgtcgc gcttgcggac catgacccag
     7021 cgcacgtagt ccaggaccgt cgcgccgtcc tggttggtgc cgaccgagtg gacatagacg
     7081 acgccggtct tgccgtcgcg gttggccttg agcccgatga cctccgactc ggccgtgatg
     7141 gtgtcacccg ggtagaccgg cacgccgaag cgccccgcgg catacccgag gttggcgacc
     7201 gcgttgagcg agacgtccgg caccgtcttg ccgaagacga cgtggaacac gagcatgctg
     7261 tccagcggca tccgctccaa ccccatggcg cgagccatcg tcacgcctga ggtcggtgcg
     7321 aaccgcggcc cgtagagcgc ggtgtagagc gcgacatcgc cgtcgctcac cgtgcgcggc
     7381 gtggcatgga ccaggcgcat gcccagcgtg aagtcctcga agaagttgcc ggtcgccgtc
     7441 ttcatctcgc ctctcctggg tgctgctgcg cacgcggtgg tgctctggtg gtgctccttc
     7501 agcgatcctc ccactcgctg tgccgagcgt gcggccgtcg acggtggcgt cgatcacgtc
     7561 agcccagcgt cgcggcgacc gtctcaagga cgcgcacgtc accggcatac gggccgtccg
     7621 ggcgaggcca gtgagtgatg acatcggtga agccgagttc gcctgcgcga cctaccattt
     7681 cggcgtagag atcggcagat tccagggcga aacgcgggga ggcgtccagc gacaggtaac
     7741 gcgcgaaacc ctccgcgcgc ccggtcgccg ccaaggcgtc gtcgagtcgc gccgtcagcg
     7801 cggccacacc ctggaaccac gcgtccagat cgtcgcctcc cgtgccggtc gtcacccagc
     7861 cctccccgta tgccgcggcg agacgcagcg cgcgcggccc gttggcagcg acgaccaggg
     7921 ggaccgacga gcgaaccggg ccgggcaggg tccgggcatc gaccgtggtg aaccatggcc
     7981 ccgagtggtc cacatgatcg gcccgcagga gccggtcgag gacggcgagg tattcggcga
     8041 accggtcaac ccgctcgcga acggtcagcg tctcgccgag gatgccggag tcgagggtgc
     8101 ctcccgtgcc gacgccgcag atgaagcggc cctcggtgag gtcgtgcagg gtgaggatgt
     8161 cgcgggcgta ggtgtagggg tggcggtagt tgggggagct gacgaaggtg cccaggccga
     8221 tccgttcggt gaccgtggcg gccgctgcca gggtcggcac ggccgcaaac caggtctggt
     8281 cgggtaggcc gctccacacg aggtggtcgt aggtccaggc gtggtcgaag ccgaagtcct
     8341 cggcagcccg ccacatgggc gcggcttcgc gccaagggtg ttcgggaagg atggtcaggc
     8401 cgtggcgcat ctgctcagtc tgcccaaggt tgaggctgca ctcgtctgag tctgcacatg
     8461 ttggcggggc cgcatggtca cggttccgac tgcgacgtcg gctccgggtg cggccccaca
     8521 tcgactgtaa ccgaaacgtg accggaacgc gcgcggttga agatgaacgg agggtgacgt
     8581 ggctcaaccc acgcttaacc ccggtcgggg gcctgcaggc gagccgcgtc ggccgaggcg
     8641 tcgtcgggca tcgtctggct catccgctcg gcctcgaccc gttgccggta gtgctcgacc
     8701 tcttcatcga ccgtcgcggc gtcccagccg aggatcggcg cgacgagctc ggcggccggc
     8761 cgtgaggcgg tgattgcgcg gtcgaacgac tcgatcgaca atcgcgtgcg gcgcccgagg
     8821 atgtcgtcca ggtgcagcgc gccctcgtgc gacgcggcat acacgatctc cgccttgagg
     8881 tagtcctccc cgccggggag cggctcgccc agcgaccggt cggccgcgac gagagcgagc
     8941 acctcggtcg tcaggacccc gtggcgcatg agcatcttct cgatccgctc gacgtgcagg
     9001 cccgactcgt gggccagccg cgcgcgacgg ttccacatgg cgcggtagcc gaccgctccg
     9061 acgaggggga tgtcctcggt cgtcgacggg gggacctcgc cgccgaggcc gcgggccgcc
     9121 tcgtcgatgg cgtcgatcgc catgacgcgg taggtcgtgt atttgccgcc ggcgacgacg
     9181 accagccctg gcgtcgggtg ggcgacgacg tgctcgcggg acagtttgga ggtctggtcg
     9241 gactcgccgg cgaggagggg acgcagcccg gcgtagacgc cttcgacgtc ggcctgggtg
     9301 agctcggtgc gcaggatccg gttgacgtgg ccgagcaggt agtcgatgtc ggccgaggtc
     9361 gccgccgggt gggccttgtc cagcgcccaa tcggtgtcgg tcgtcccgat gatccagtgc
     9421 cgtccccacg ggatgacgaa gaggaccgac ttctcggtgc gcaggatgat gccgctcttg
     9481 gagtggatac ggtcacgcgg gaccacgagg tgcacgccct tggatgcccg cacgtggaat
     9541 tggccgcgct cgccgaccag cgcttgggtg tcatcggtcc acacaccggt cgcgttgacg
     9601 acctggcggg ccaggatgtc gaactcgcgg ccggtcacct tgtcgagtgc tcgcacccca
     9661 gtgacccgct cgccctgacg caaaaaccct actgccgcaa cgcgggtcgc gacgtgggcg
     9721 ccgtaggacg ctgccgtgcg agcgagctcc atggtatgcc gcgcgtcgtc caccttcgcg
     9781 tcgtagtact gcacggcacc gaccagcgcg gtggacttga gggccggcgc gatcttgagc
     9841 gcctggcgcc tggtgaggtg cttgtgccag ggcagcccgg ccgagtgccg cgacgcccgg
     9901 gccatcgagt cgtagagcgc cagaccggcc tcgacgtagg gccgctcgac caccggtttg
     9961 gtcagcgggt agaggaacgg caccggtcgc accaggtgag gggcgaggcg ggtgagcagc
    10021 agcccgcgct ccttgagcgc ctcggcgacg agccggaagt cgaacatctc caggtagcgc
    10081 aagccgccgt ggatgagctt gctgctgcgg ctggacgtgc ccgaggcgaa gtcgcgcttc
    10141 tcgatgagcc ccgtgcgcag gccccgggtc accgagtcca gggcgcagcc ggcgcccgtc
    10201 accccaccgc cgatgacgag gatgtcgagt tcctcgcccg aggcgaggcg gtcgatggcg
    10261 gctgaacgat aagcggggga cagcgcggtt ggagtgctca ccgtcccatc ctcgcaaatc
    10321 tctacgccgg ccggaccggt gccccgggtc ccgtcagccg gagacgggcg cccagtcgag
    10381 agtgcgggag acggccttgc gccacgcggc atacccgctg gcgcgttgct cgtccgtcca
    10441 ctgcggcgac cagcgacggt cctcctgcca gttggcgcgc aactcgtcgg tcgtgcgcca
    10501 gaagccgacc gccagacccg cggcgtaggc ggcccccagc gcggtcgtct cggcgacgac
    10561 cggcttgctc acctggacgc cgaggatgtc ggcttggatc tgcatgcaga gggcgttggc
    10621 cgtgacgccc ccgtcgacct tgagggtgtc caggcggaca ccggagtcgg cgaacatcgc
    10681 gtcggccacg tccttggtct ggtagcagat cgcctcgagg gtcgcgcggg ccaggtgtgc
    10741 gttggtgttg aagcgcgaga gcccgacgat cgcgccgcgg gcgtccgagc gccagtaggg
    10801 agcgaagagg ccggagaagg cggggacgaa atagaccccg ccgttgtcgg gcacctgcgc
    10861 ggcgaggcgc tcgatgtcgg cggctccgct gatgatgccg agttggtcgc gcagccactg
    10921 cacggccgat ccggtcacgg cgatggagcc ttccagcgcg tatgccgcct gctcgccagc
    10981 gaactggtag gccacggtcg tcagcagtcc agctttcgag cggactggct cggtgcccgt
    11041 gttgaggagc atgaaattgc ctgtgccgta agtgtttttg gcctcgccgg gagcgaagca
    11101 gacttgcccg acggtggcgg cttgttggtc gccgaggtcg cccgcgatct tgatggggcc
    11161 gccccaggcg ttgctgccgc ccgacgtcgc ggtgacgccg tagcggtcgg catcgccgct
    11221 cggcaggatc gtcggcagca tcgcgcgggg cacgttgaag aagccgagga gctcgtcgtc
    11281 ccagtcgagg gtgcgcaggt ccatgagcat cgtgcgactg gcgttggtga cgtcggtgac
    11341 atggcgcccg ccgtgcggac cgccggtgag gttccagatg acccaggtgt cgggggtgcc
    11401 gaagagggcg tcgcctcgct cggccgccgc ccgcaggccg tcgacgtgct ccagcagcca
    11461 ctgcagtttg ccgccggaga agtaggtggc cggggggagc ccggcgcgct cgcggatcac
    11521 ctcgccgcgc tcgtcggcgt cgagggccgc ggcgatggcg tcggtccgcg tgtcctgcca
    11581 gacgatcgcg tggtgccacg ggcgtccggt atgccggtcc cagacgatcg tcgtctcgcg
    11641 ctggttggtg acgcccatgg cggccaggtc gctggcgtgc aggccagctg tggtgagcgc
    11701 cgagccgatg acggcctggg tgcgctccca gatctccagg gggttgtgct cgacccaccc
    11761 ggcccggggg aggatctgct cgtgttcgag ttggtgcttg cccacctcgc gcccggcatg
    11821 gtcgaagatc atgaaccgcg tgctcgtcgt cccttgatcc agggcgccca cgaagtcagc
    11881 catagccaca gattggcaca cgccccccgc ccctgcaccc ctgggcttgc agccccagca
    11941 ccccccc
//
LOCUS       DAORPW010000068        11842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1161_length_11842_cov_748.652244, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000068 DAORPW010000000
VERSION     DAORPW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11842
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1161_length_11842_cov_748.652244"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1779)
                     /locus_tag="PLB86_07115"
     CDS             complement(181..1779)
                     /locus_tag="PLB86_07115"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HRC64944.1"
                     /translation="MPRTSSVPASPTASASSPREAPFAVIVQAADAVAATTSRTAKIG
                     LLADVLGRVTVDEIEPAIGFLTASVRQGRLGVGWRTIADLAEGAAVPADQASLTVTEV
                     DAAIERLAALGGTGSAAARTEELTRLWGRATADEQRLLTGILLGELRIGALEGVLTDA
                     VAKAAGRPLEEVRRAAMLTGSLGRTAYLALTGADLAQVGLTVGVPLLPMLAATASSPG
                     EAVTALGPAPVSVEVKLDGARIQVHRHGGPGSRVEVFSRTLADITARVPELVEVVSGF
                     PGGSLILDGETLTLDEDGAPRPFQETMSRFGSTGARAEILRPWFFDILHADGVDLIDE
                     PLSVRRERLREAVGEHGILALETADPVAAETFSQEALAAGHEGVLVKALDSPYAAGRR
                     GRQWLKVKPVHTYDLVVLAAEWGSGRRTGWLSNLHLGARDPGVPVGEPGAFVMVGKTF
                     KGLTDEVLRWQTTRLLELETKRTRYAVHVTPALVVEIAIDGVQRSSRYPGGLALRFAR
                     VKGYREDKTVADADTIESLRALLR"
     gene            complement(1794..2075)
                     /locus_tag="PLB86_07120"
     CDS             complement(1794..2075)
                     /locus_tag="PLB86_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRC64945.1"
                     /translation="MHGYSERKDAHLKRLRRIEGQVRGVSRMVEEDQYCIDVLTQLSA
                     VTRALQSVALGLLDDHMAHCVVAAAREGGVEQETKLAEASAAIARLVRS"
     gene            complement(2143..3474)
                     /locus_tag="PLB86_07125"
     CDS             complement(2143..3474)
                     /locus_tag="PLB86_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRC64946.1"
                     /translation="MSLAAGAAAGLAACASPGTGAPGAGGTTGSGAGAGSGAGLGADV
                     NLAMSKVARATPAAGAADPAAKAITGFTERMVVSVLAGSSDNLVCSPYSVAAALAMTA
                     NGARGKTATEMLAVLGGLTIDDLDAGLAALSATFASRAGAKTKSDGNKADIALDIANS
                     LWGQRGTTWEQPFLDALAKWFGAGMNLVDYVAATEAARARINGWTAAATHDKINNLLP
                     EGALDPSTRLVLVNAVYLKAPWEEPFAASATQKAPFTLAAGGTVQADMMRGSFERASA
                     ATGPGYTAVSLPYAGGELAMTLVLPGRSNPESLIEWLKSGLLSTALADLRPRPLHLAM
                     PKWTFRSRLALKPVLSALGMPTAFEGSADFSGITREVQLAIDEVFHQGFVAVDEEGTE
                     AAAATAVVMSEVSAPADPLQVTLDRPFLFVVHDVASRTPLFIGRVADPTRT"
     assembly_gap    3843..3942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3980..4960)
                     /locus_tag="PLB86_07130"
     CDS             complement(3980..4960)
                     /locus_tag="PLB86_07130"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HRC64947.1"
                     /translation="MVSETVAHNGLPGPLPGSPPRRAGGVRRTTSLDLTRPLGPDGPI
                     EVNGRARDVRTADAGAMTVLDKALLSLTVTDGIVTRLRSFPERAAAPRLVGRQALVGW
                     RTGLWRELHDDVESGSALHLLLDDLPGGMVVGGFTRRRALAAAGEPVPQPGRRLDVCA
                     GWAADSRAAQIMADTGTPPPPVTPEAPDLVRDDDPNGWHTLAALPPWAMSRRRRVDAH
                     LDGAQIEVDAMFRDSFIDIEGRERVLHEYAVHATLEANEGRVLAVQPAPRVLPHLECP
                     VAGASTQRLIGMPCADLRDLVSSTLFGPSTCTHLNDLMRSIADVPALLTR"
     gene            5377..5745
                     /locus_tag="PLB86_07135"
     CDS             5377..5745
                     /locus_tag="PLB86_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRC64948.1"
                     /translation="MTHVPVPLYQAKAELFRTLGHPARIRVLELLSERDYAVHELLAA
                     IELEPSSLSQQLAILRRTGLVRQRRVGGEVVYSIVVPATKDLLSAGRNVLRAVLADQD
                     QLADLLDPPVLKTPRRGGRG"
     gene            5742..6230
                     /locus_tag="PLB86_07140"
     CDS             5742..6230
                     /locus_tag="PLB86_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRC64949.1"
                     /translation="MSALDIAKLLRRVGRITETDTAASPSDYTPPAALAGSVQVRHVD
                     CGSCNGCEIELAGAFGPVYDAERYGARLVASPRHADVLAVTGAPTRNLAEPLRRSVEA
                     APRPRVVLAIGDCAAGEGAYAQGYAVVGGVADIVPVDLVVRGCPPPPDAIVAALRQVT
                     GK"
     gene            6227..8245
                     /locus_tag="PLB86_07145"
     CDS             6227..8245
                     /locus_tag="PLB86_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRC64950.1"
                     /translation="MTLTDVGLLSQPALAGLAFIAALAVNDSRRSAIAGTLNALLGLA
                     TAATGVGALLGEPGSLTFATGIPGIAHLGQLALAPDRLGGIFLVVVGVVGALSAWFGV
                     GYAHGATATRAAWAAYPIFLWGMSIVPAAGDAVTFLLGWELMALGSTVLLHSEHATRP
                     QVRSAVLWYAAMTHLSFVLLLAGFAVLIASANGTTWEALAASPPTGVSASLAFVLLAG
                     GFATKAGLVPVHVWLPRAHPEAPSHVSAAMSAAMVKLGIYGLLLVTLRLLPDGPRWWA
                     IGLLVLGGVSAVYGILEASVTSDLKRLLAFSTTENLGLMVTGLAVAMMLRSTGQRAVA
                     DVALVAVVLLVISHAAFKTVLFLGAGSILHATGERDLDRLGGLARRMPRTAFATSIGA
                     LAAAALPISSGFVAEWVLLQSLVHGDTRGPTADRVIAIALPITVAVVALTAGLALLTF
                     VKVVGIAFLARGRSPEATNAHENPASMTLPMWVGAGLVLGLGLVPGWVAMVAAAAVGA
                     SGIEPAGLAGVALPSVNALLDPVSLVLMSLAVAVPVAVVTVRAARKAPRRTVDLPWGC
                     GGVRVDPTMQYTATSYAEPLVRIFDDALRPTRDLDVTHTVESDYLVREVAYRQRMVDQ
                     VERGLYEPVIRLLWRIGDQARRLQDGSVHRYLGFSFAALVVVLLVVTW"
     gene            8242..9180
                     /locus_tag="PLB86_07150"
     CDS             8242..9180
                     /locus_tag="PLB86_07150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRC64951.1"
                     /translation="MTALAVAWQILVAVALTPVLVGVMRQVRARLEGRVGAGVGQPWR
                     DVRKLLAKEPLAAEGAGPLMSAVPVILVISSLVSVALVPLLGIATLAWVPDDLFVVVS
                     VLLVGTVALALLGLESGTAFGGMGSSRHMMIAALVEPTVLVAVYALSVPVGTTALSSI
                     LQARMSDPTTVISPVSLLALVALSIVVLAETGRLPVDNPATHLELTMVHEAMILESSG
                     RDLGWLELGSWLRLTALLGLVVNLALPWGVAADLGVGATVIATLAFSVKLLIAGSMIA
                     AAEVFLAKVRLFRVPELLAGSFVLAFLSVTASYLLV"
     gene            9188..9847
                     /locus_tag="PLB86_07155"
     CDS             9188..9847
                     /locus_tag="PLB86_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64952.1"
                     /translation="MTESLFAQTVATATGLLMLTAVLQVWRRSTDASTRLLAVQGAAL
                     ALLVGAIGLAEGHAELVAVAVVVFALKGVLLPWVLARTIARSGIHREDSSAVNPVSGL
                     LAAAGLATLAYAVSRPLVALDAGPAAHAVPAGLTLVLVGFLVLLTRHRAVSQVIGFLV
                     IDNGIATVAFLTAGGVPLVVELGVLLDVVLVVLILRILSARLELAHGELDLHDLRELH
                     D"
     gene            9847..11298
                     /locus_tag="PLB86_07160"
     CDS             9847..11298
                     /locus_tag="PLB86_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRC64953.1"
                     /translation="MSLFLWIPLLVPAACAAAAATRRQAPWTRPLGLASALVILVTGL
                     GLLGSITAAGGEALTAAGDLFRVDALAAYLLMTIGAVATVALWADLSVPAEPDGWFTA
                     LVCAFLAAMTLAVLADNLGVLWVAIEATTITTAFLVGYRSGRRSLEAAWKYVVLGSVG
                     VAIAFLGIVVLYAATRSAGHPTLSWVELVRLAPQLDPAVTRLGIALATLGFATKAGLA
                     PMHSWLPDAHSQAPAPVSGLMSGVLLSVALYAVLRLRTISDLALGPGFMRGLLLVLGV
                     LSLLVAATLTLTQRDYKRLLAYSSIEHMGLMAVGVAIGGTLATTAVLVHILGHGLVKS
                     AMFVQSGRILRAEGSSKVADVVGLSARRPDLAGPLLLGTAALLGFPPFVLFFTEVAII
                     IAGWAAGLAPVMLIVLALLLLTFIGMSRQVLAMTLGPAPDRSVDDSTVARLPRKAQAP
                     VTVALGAAVAAGLVALPAAAALGAAVTGLGVTP"
     gene            11295..>11842
                     /locus_tag="PLB86_07165"
     CDS             11295..>11842
                     /locus_tag="PLB86_07165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRC64954.1"
                     /translation="MSGERAAYREDVVPGALGGKVQALLGSGWRLALVAGHDDGDGFR
                     VVYSFLRPVGQRRELVVRLPHDQPELPSLAGLSYPAGRFERELRDLFGIEPVGHPLPR
                     GLVRHGHWPTTWHPMLRDAGPMPDFEPDVGSYPFVHVQGDGVYEIPVGPIHAGIIEPG
                     HFRFSVVGETILRMKARLWFVHR"
BASE COUNT         1531 a         4342 c         4026 g         1843 t
ORIGIN      
        1 cggccgagcg acgtgcggca ctcatcgccg aggtcggcgc ccagcgagag cggcgctaga
       61 ggccgtcggg gtgccatcca ggacatccgg ttggcacccg agttgatcat cggcgagtca
      121 agcccactca gccagacccg agatgcgtcg cgtgcctcca cccggctctg gccctctcgc
      181 tcagcgcagg agggcccgca gcgactcgat ggtgtcggcg tcggccaccg tcttgtcctc
      241 ccgatagccc ttgacccgcg cgaaccgcag cgccagccca cccggatagc gcgacgagcg
      301 ctgcacgccg tcgatggcga tctcgacgac gagcgcgggg gtgacgtgca cggcataccg
      361 ggtgcgcttg gtctccaatt cgagcaaccg cgttgtctgc caacggagca cctcgtcggt
      421 gagccccttg aacgtcttgc cgaccatgac gaaggcgccc ggctccccga ccggcacccc
      481 cgggtcgcgc gctccgaggt ggaggttcga cagccacccg gtgcgccgcc ccgagcccca
      541 ctcggccgca agcacgacga ggtcgtaggt gtgaaccggc ttgaccttga gccactgacg
      601 gccgcggcgc cccgcggcat acggggaatc gagcgccttg accagcacgc cttcgtgacc
      661 ggcggcgagc gcctcctgcg aaaacgtctc cgccgcaacg ggatcggcag tctccagcgc
      721 gagtatgccg tgctccccca ccgcctcgcg cagccgctcc cgacgcaccg acaacggctc
      781 gtcgatgagg tcgacaccgt cggcgtgcag gatgtcgaag aaccacggcc gcaggatctc
      841 ggcgcgcgca cccgtcgacc cgaagcggct catcgtctct tggaacggcc gcggcgcgcc
      901 gtcctcgtcg agcgtgagcg tctcgccgtc gaggatgagc gatccgcccg ggaagccgga
      961 cacgacctcg acgagctccg gcacccgcgc ggtgatgtcg gccagggtcc ggctgaacac
     1021 ctcgacccga ctgcccggcc cgccatggcg gtgcacctgg atccgagcgc cgtcgagctt
     1081 gacctcgacc gacaccggcg ccggcccgag tgccgtcacg gcctcgccgg gcgaggaggc
     1141 cgtcgccgcc agcatcggca gcagggggac gcccaccgtg agccccacct gtgcgaggtc
     1201 ggcaccggtc agcgcgaggt atgccgtccg ccccaggctc ccggtcagca tcgccgcccg
     1261 ccggacctcc tccagagggc gcccggcagc cttcgccacc gcatcggtga gcaccccttc
     1321 tagcgccccg atccgcaact ctcccaagag gattcccgtg agcaaccgct gttcgtcggc
     1381 cgtcgcgcga ccccagagcc gggtgagttc ttcggtccgc gccgccgccg atccggtgcc
     1441 tccgagagcg gccagccgct cgatcgcggc gtcgacctcg gtgaccgtga gagaggcctg
     1501 atcggcggga actgcggctc cttcggcgag gtcggcgatg gtccgccagc cgacaccgag
     1561 gcgcccctgg cgcaccgagg cggtgaggaa gccgatcgcc ggctcgatct cgtcgaccgt
     1621 caccctgccc aggacgtcgg ccagcagccc gatcttcgcc gtccgcgagg tggtcgccgc
     1681 aacggcgtcg gcggcctgga cgatcacggc gaacggcgcc tcacgcggcg acgacgccga
     1741 ggcggtcggg gacgcgggca cagacgaggt gcgcggcata ccggcgaagg aactcagctg
     1801 cgcacgaggc gcgcgatggc tgcggaggcc tccgcgagtt tggtctcctg ctcgacgccg
     1861 ccctcccgcg cggcggcgac gacgcagtgc gccatgtggt cgtcgagcag ccccagcgcg
     1921 acggactgca gcgcgcgggt caccgccgac aactgggtga ggacatcgat gcagtactgg
     1981 tcctcctcga ccatccggga caccccccgg acctgcccct cgatacgtcg cagccgcttg
     2041 aggtgcgcat ccttgcgctc ggaatacccg tgcatgtcgc ctcctgctcg tgataccccc
     2101 acagggtatg ccgcgcagct cccgttcgcg gtgtggcgcg acctaggtgc gagtcgggtc
     2161 ggcgacgcgt ccgatgaaca ggggtgtgcg actggcgacg tcgtggacga cgaagaggaa
     2221 cgggcggtcc aaggtcacct ggagaggatc cgcgggcgcc gagacctcac tcatgaccac
     2281 agcagtggcg gcagcggcct cggtgccttc ctcatcgacg gcgacgaatc cctggtggaa
     2341 cacctcgtcg atggcgagtt ggacctcgcg ggtgatccct gagaagtcgg ccgacccctc
     2401 gaaagcggtc ggcatcccca gcgccgacaa caccggcttg agcgcaaggc gactgcggaa
     2461 agtccacttc ggcatggcga ggtggagcgg gcgcgggcgc aggtctgcga gcgccgtgct
     2521 caggaggccg gacttgagcc actcgatgag ggactcgggg tttgagcgac ccgggagcac
     2581 cagggtcatg gcaagttcgc ccccggcata cgggagcgag acggcggtgt agccggggcc
     2641 ggtggccgcc gacgcccgct cgaacgagcc cctcatcatg tcggcctgca cggtgccgcc
     2701 ggccgccagg gtgaagggcg ccttctgggt ggccgaggcg gcgaaaggct cctcccacgg
     2761 cgccttgaga tatacggcat tgaccaggac gagtcgggtc gacgggtcga gcgcaccctc
     2821 cgggaggaga ttgttgatct tgtcgtgggt cgccgccgcc gtccatccat tgatgcgagc
     2881 gcgggcggct tcggtggcgg ccacgtagtc gacgaggttc atcccggcgc cgaaccactt
     2941 cgccagggcg tccaggaacg gctgctccca cgtcgtgccc cgttgtcccc acagggagtt
     3001 ggcgatgtcc agggcgatgt cggccttgtt gccgtcggac ttggtcttgg ctccggcgcg
     3061 gctggcgaag gtcgccgaca gcgcagccag cccggcatcg agatcgtcga tcgtcagccc
     3121 gcccagtacg gcgagcatct cggtcgcggt cttgccgcgg gcaccgttgg ccgtcatcgc
     3181 gagcgccgct gccaccgaat agggcgagca gacgaggttg tcggaactcc ctgcaaggac
     3241 agagacgacc atccgctcgg tgaaaccggt gatggctttg gcggccgggt ccgccgcgcc
     3301 ggctgccggg gtggctcggg cgaccttcga catggccagg ttcacgtctg cgcccaatcc
     3361 agcgccagac ccggctccgg cgccgctgcc ggtggttccg cccgccccag gggcaccggt
     3421 tccaggcgag gcgcaggccg cgaggcccgc ggccgcgccg gcggccaggc tcatccagcc
     3481 gacgagccgg ccgaggtccc gacgagagag atctaaacgg gggaagtccc gatgtgtctc
     3541 ccgaccggta agggcgggag gtatgccgtg actgggttcc atgaagggtt ggacgtcgag
     3601 agccgaccct cggttcctgc cgaagcgccc gtcctcaacg tcgtcactct cacaagagtc
     3661 accacaggca aggtcaccgc agaggagtcg ccgcggacga ggtcaccgca gggcctgcag
     3721 ctggacgacc tgggcgggca tggtctggat caccctgcgg gccatcgtcc ggacccggtc
     3781 gtcgccggtc cgccgcacca ccgacccggc gaactcggca gcaccgtcct ggtgagcgat
     3841 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttgcccca cgcgcccgac
     3961 gccctcgggc gcaggaaact caacgggtca gcagggcggg aacgtcggcg atcgagcgca
     4021 tgaggtcgtt gagatgggtg caggtcgacg gcccgaacag cgtgctggag acgaggtcgc
     4081 gaaggtcggc acacggcata ccgatgaggc gctgggtgga tgctccggcg acgggacatt
     4141 ccaggtgtgg gagcacccgc ggtgccggtt ggacggcgag gacccgaccc tcgttggcct
     4201 ccaaggtggc gtggaccgcg tattcgtgca ggacccgttc gcgcccctcg atgtcgatga
     4261 acgagtcgcg gaacatcgcg tcgacctcga tctgggcccc gtcgaggtgg gcatcgaccc
     4321 ggcggcggcg cgacatcgcc cacggcggca gcgcggccag cgtgtgccag ccgttggggt
     4381 cgtcgtcacg gaccaggtcg ggggcctcgg gggtgaccgg cgggggcggg gtgccggtgt
     4441 cggccatgat ctgagcggcc cgcgaatcgg ccgcccatcc ggcgcacacg tccaggcgtc
     4501 gcccgggttg gggcaccggc tcgcccgccg cggcgagcgc ccgccgccgg gtgaacccgc
     4561 cgacgaccat gccgccggga aggtcgtcga ggagcaggtg cagcgccgac cccgactcga
     4621 cgtcgtcgtg caactcgcgc cacagaccgg tccgccagcc cacgagcgcc tggcgaccga
     4681 cgaggcgcgg cgcagcggcc cgctccggaa aactccgcag ccgggtgacg atgccatcgg
     4741 tgacggtcag cgacagcaga gccttgtcga gcacggtcat ggcgcccgcg tcggcggtgc
     4801 gcacgtctcg ggcccggccg ttgacctcga taggcccgtc agggccgagt ggccgagtca
     4861 ggtcgaggct ggtcgtgcgc cgcacgcccc ccgctcgacg cggcggcgag ccgggcaggg
     4921 ggccgggaag cccgttgtgc gccaccgtct cgctcaccat cccccgaggg tatgccgccc
     4981 gcccaccgcc cgcccacggc ctgtccaccg ccgacacaac ggcaggtgcg tcatgccgaa
     5041 cgcgagatag gttgcgcgtc tggcatgacg caactcgccg gggctcgact tgaatacccc
     5101 caggggtata tgtcaccata gccccacacg acgacaagga ggctccacga tgtgcagtcc
     5161 ggctcgctgc tccacctgca acaaggtcac ctggcggggg tgcggcatgc acgtcgacgc
     5221 cgtcatggcc cagttccccg tctcagagcg ctgcacctgc cgcgagaccc agtccaggac
     5281 cccaacgcct cagcgctgac cggcccactt ggccgaccct tcctcatgtg ccagacttga
     5341 acagttgcgc aattggtcac atgaacaggt gaaggggtga cgcacgtgcc ggtcccgctc
     5401 tatcaggcca aggccgagct cttccgcacc ctgggccacc ccgcgcggat ccgggtgctg
     5461 gagctgctct ccgagcgcga ctacgccgtc cacgaactcc tggccgccat cgagctcgag
     5521 ccgagcagcc tctcgcagca actcgccatc ctccggcgca ccggcctcgt gcgccagcgt
     5581 cgagtggggg gcgaggtggt ctattcgatc gtcgtcccgg ccaccaaaga tctgctgagc
     5641 gccggccgaa acgtcctgcg cgcggtcctc gccgaccagg accagctggc cgacctgctc
     5701 gatccgccag tgctcaagac ccctcggcgg ggtggccgcg gatgagcgcg ctcgacatcg
     5761 ccaaactcct gcgtcgcgtc ggtcggatca ccgaaaccga caccgccgca tcaccatccg
     5821 actacacgcc acccgcggcc ctcgccggga gcgtccaggt caggcacgtc gattgcggct
     5881 catgcaatgg gtgcgagatc gagctggcgg gcgcctttgg cccggtctac gacgccgagc
     5941 gctacggggc ccggctcgtc gcctcgcccc ggcacgccga cgtcctggcc gtcactggcg
     6001 caccgacccg caacctcgcc gagccgttgc gccgcagcgt cgaagccgca ccccgcccga
     6061 gggtcgtcct cgccatcggc gactgcgccg ccggggaggg cgcctacgcc caggggtatg
     6121 ccgtcgtcgg cggcgttgcc gacatcgtcc ccgtcgacct cgtcgtgcgc ggctgcccgc
     6181 cgcctcctga cgccatcgtc gccgcactgc gacaggtcac tggcaaatga ccctcaccga
     6241 cgtcgggctg ctcagccagc cggcgctggc aggcctggcc ttcatcgctg cgctggccgt
     6301 caacgactcc cgccgctcgg ccatcgccgg cactctcaac gcgctcctcg gcctggccac
     6361 ggccgccacc ggcgtcggag ccctgctcgg tgagcccggc tcgctgacct tcgccaccgg
     6421 catccccggg atcgcccacc tcggccaact cgcccttgcc cccgaccgac tcgggggtat
     6481 cttcctcgtc gtcgtcgggg tcgtcggggc cctcagcgcc tggttcggag tcggctacgc
     6541 ccacggcgcc acagcgacgc gagccgcgtg ggcggcatac ccgatcttcc tctggggcat
     6601 gtcgatcgtg cccgcggccg gcgacgcggt gaccttcctg ctggggtggg agttgatggc
     6661 gctcgggtcg acggtcctgc tgcactccga gcacgcgacc cggccgcagg tgcgctccgc
     6721 cgtcctctgg tatgccgcga tgacgcacct gagcttcgtc ctgctcctgg ccggcttcgc
     6781 ggtcctcatc gcgtcggcca acggcacgac ctgggaggcc ctcgccgcct cgccgccgac
     6841 cggggtgagc gcttcgctgg ccttcgtgtt gctcgcgggc gggttcgcca ccaaggcggg
     6901 cctggtgccg gtgcacgtct ggctcccccg cgcccacccc gaggcgccca gccacgtgtc
     6961 ggccgcgatg agtgcggcga tggtcaaact cggcatctac gggctgctgc tggtcaccct
     7021 gcgactgctg cccgacgggc cgcggtggtg ggccatcggg ctgctcgtcc tcggcggggt
     7081 gtcggcggtc tacggcatcc tcgaggcctc ggtcacgtcg gatctcaaac ggctgctggc
     7141 cttctcgacc accgagaacc tcggcctcat ggtcaccggc ttggccgtcg cgatgatgtt
     7201 gcgcagcacc ggccagcgcg ctgtcgccga tgtcgcgctc gtggccgttg tgctcctcgt
     7261 catcagccat gccgccttca agacggtgct gttcctcgga gccgggtcga tcctgcacgc
     7321 gaccggcgaa cgcgacctcg accggttggg agggctggcc cgacggatgc cgcggaccgc
     7381 cttcgccacc tcgatcggag ccctcgccgc ggccgccctc ccgatctcct cgggcttcgt
     7441 cgccgagtgg gtcttgttgc agtcgctggt ccatggcgac acccgaggcc cgaccgccga
     7501 ccgggtcatc gccatcgccc tgccgatcac cgtcgccgtc gtggcgctca ccgcgggcct
     7561 cgccctgctg accttcgtca aggtcgtcgg catcgccttc ctcgcccgtg gacgatcgcc
     7621 cgaggccacg aacgcccacg agaacccggc ctcgatgacc ctgcccatgt gggtcggagc
     7681 cgggctcgtc ctcgggctgg ggttggtgcc cgggtgggtg gcgatggtgg ccgcagccgc
     7741 cgtgggtgcc tccggcatcg agccggccgg gttggccggg gtcgccctcc cgagtgtcaa
     7801 cgccctgctg gacccggtgt ccctcgtgct catgtcgctc gccgtggcgg tgccggtcgc
     7861 cgtcgtcacc gtgcgggcgg cgcgcaaggc cccgcggcgc acggtcgacc tcccctgggg
     7921 gtgcggcggc gtccgcgtcg acccgacgat gcaatacacc gcgacctcgt atgccgagcc
     7981 cctcgtccgg atcttcgacg acgccctgcg accgacccgc gacctcgatg tcacccacac
     8041 ggtggagtcg gactacctcg tgcgcgaggt tgcctatcgc cagcgcatgg tcgaccaggt
     8101 ggaacgcggc ctctacgagc ccgtcatcag gctgctctgg cggatcggcg accaggcccg
     8161 gcggctccaa gacggcagcg tgcaccgtta cctcgggttc tcgtttgcgg ccctcgtcgt
     8221 cgtcctgttg gtggtgacct ggtgaccgcc ctcgccgtgg cctggcagat cctcgtcgcc
     8281 gtcgccctca caccggtcct cgtcggggtc atgcgtcagg tgcgcgcccg cctcgaaggc
     8341 cgcgtcggtg ccggggtcgg gcagccgtgg cgcgacgtgc gcaaactgct ggccaaggag
     8401 ccgctcgccg ccgagggggc cggaccgctc atgtccgcag tccccgtcat cctcgtcatc
     8461 agcagcctcg tcagcgtggc cctcgtgccg ctgttgggga tcgccaccct ggcctgggtt
     8521 cccgacgacc tgttcgtcgt cgtctccgtc ctgctcgtcg gcaccgtcgc cctggccctg
     8581 ctcgggctcg agtcgggaac ggccttcggc ggcatggggt ccagccggca catgatgatc
     8641 gccgcgctcg tcgagccgac ggtcctcgtg gccgtgtatg ccctctcggt cccggtcggc
     8701 acgaccgccc tgtcctcgat cctgcaggcc cggatgtccg acccgacgac cgtcatctca
     8761 ccggtgagcc tgctggcgtt ggtcgccttg agcatcgtcg tcctcgccga aaccgggcgg
     8821 ctgcccgtcg acaacccggc cacgcacctc gagctgacga tggtccacga ggcgatgatc
     8881 ctcgaaagca gcggccgcga tctcgggtgg ctcgagttgg gctcctggct tcgcctcacc
     8941 gcgctgttgg ggctcgtcgt caacctcgcc ctgccgtggg gggttgccgc cgacctgggc
     9001 gtcggagcca ccgtcatcgc caccctcgcc ttcagcgtga agttgctcat cgctgggtcg
     9061 atgatcgccg cggccgaggt gttcctcgcc aaggttcggc tgttccgggt gcccgagttg
     9121 ctcgccggct cgttcgtcct ggccttcctg tccgtcaccg cctcctacct gctcgtctga
     9181 gaccaccatg accgagagcc ttttcgccca gaccgtcgcc accgcgaccg gcctgctcat
     9241 gctcacggcg gtgctgcagg tgtggcgccg ctcgaccgac gcctcgaccc ggctcctggc
     9301 cgtccagggg gcggcgctgg ccctcctcgt gggcgcgatc ggcctggccg aagggcacgc
     9361 cgagctcgtc gctgtcgccg tcgtcgtctt cgccctcaag ggcgtcctgc tgccgtgggt
     9421 gctcgcccgc accatcgcgc gctccggcat ccaccgggag gactcctcgg cggtcaaccc
     9481 cgtgagcggg ctcctcgctg cggccggact ggccaccctc gcgtatgccg tgagccgccc
     9541 cctcgtcgct ctcgacgccg gccccgccgc tcacgccgtt cccgccgggc tcaccctcgt
     9601 cctcgtcggt ttcctcgtcc tgctcacccg ccaccgcgcg gtcagccagg tcatcgggtt
     9661 cctcgtcatc gacaacggga tcgccacggt cgccttcctc accgccggcg gggtgccgct
     9721 cgtcgtcgag ctcggggtgc tgctcgacgt cgtcctcgtc gtgctcatcc tgcggatcct
     9781 ttcggcccgg ctggagttgg cccatggcga gctcgatctg cacgacctga gggagctgca
     9841 cgactgatga gcctcttcct ctggatcccc ctcctcgtcc cggccgcgtg cgccgcggca
     9901 gccgccacca ggcgtcaggc cccctggacc cggcccctcg ggctcgcctc cgccctcgtc
     9961 atcctcgtca cgggcctcgg cctgctcggc tccatcaccg ccgccggggg cgaggccctc
    10021 accgcggcag gtgacctgtt ccgggtcgat gcgctcgcgg catacctgct catgacgatc
    10081 ggcgcggtgg cgactgtcgc gctctgggcc gacctctcgg tgcccgccga gcccgacgga
    10141 tggttcaccg cgctggtgtg tgcgttcctc gcggcgatga ccctcgcggt cctcgccgac
    10201 aacctcggcg tgctctgggt cgccatcgag gccacgacga tcacgaccgc cttcctcgtc
    10261 ggctaccgca gcgggcggcg gtcgctggag gcggcgtgga agtacgtcgt gctcgggtcg
    10321 gtcggcgtcg ccatcgcgtt cctcggcatc gtcgtcctgt atgccgccac ccgctccgcc
    10381 ggtcacccca ccctctcgtg ggtcgagctc gtccgcctcg cgccccagct cgaccccgcg
    10441 gtcacccggc tcggaatcgc gctggcgaca ctgggattcg cgaccaaggc cgggctcgcg
    10501 ccgatgcact cgtggctccc cgacgcgcac agccaggcac cggcccccgt gtccggcctc
    10561 atgtccgggg tgctgctgtc cgtcgccctg tatgccgtgc tgcggcttcg cacgatcagt
    10621 gacctcgcgc tcgggccggg tttcatgcgg ggcctgctgc tggtgctcgg ggtgctgtcg
    10681 ctcctcgtgg cggccacgtt gacgttgacg caacgggact acaagcggct gctggcctat
    10741 tcgagcatcg aacacatggg gctcatggcc gtcggggtgg cgatcggcgg gacgctggcc
    10801 acgacggccg tcctcgtcca catcctcggc cacggcctgg tcaagtcggc gatgttcgtg
    10861 cagtccgggc ggatcctgcg cgcggagggc agcagcaagg tggccgatgt cgtcgggttg
    10921 agcgcgcggc gccctgatct ggccgggccg ctcttgctgg gaacggctgc gctgctgggc
    10981 ttcccgccgt tcgtcctgtt cttcaccgag gtggccatca tcatcgccgg gtgggcggcc
    11041 gggttggcgc cggtgatgct catcgtcctg gcgctgctgc tgctgacgtt catcggcatg
    11101 tcccgacagg tgctcgcgat gacccttggc cccgctccgg accgatcggt ggacgactcg
    11161 acggtggctc ggttgccgcg gaaggcacag gcccccgtca cagtcgccct cggggccgcc
    11221 gtcgcagccg ggctggtcgc gttgccagcc gcagccgcgt tgggcgcggc cgtcacgggc
    11281 ctgggggtga cgccgtgagc ggtgagcggg cggcataccg ggaggacgtc gtcccgggcg
    11341 cgctgggcgg caaggtgcag gcgctcttgg gctcggggtg gcgactggcg ctggtcgccg
    11401 ggcacgacga cggtgacggc ttccgggtcg tctacagctt cttacggccg gtcggtcagc
    11461 ggcgcgagct cgtcgtgcgc ctcccccatg accaacccga gctgcccagc ctggccgggc
    11521 tgtcctatcc cgcagggcga ttcgagcgcg agctgcgcga cctgtttggg atcgagccgg
    11581 tcgggcaccc tctgccgcga gggctggtcc ggcacggaca ctggccgacg acctggcacc
    11641 cgatgctgcg cgacgccggc ccgatgcccg atttcgagcc cgacgtcggg tcctatccgt
    11701 tcgtccatgt ccagggtgac ggggtctacg agatcccggt gggcccgatc cacgcgggca
    11761 tcatcgagcc ggggcacttc cggttctcgg tggtcggcga gaccatcctg cggatgaagg
    11821 cccggctgtg gttcgtccac cg
//
LOCUS       DAORPW010000069        11841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1162_length_11841_cov_784.779484, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000069 DAORPW010000000
VERSION     DAORPW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11841
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1162_length_11841_cov_784.779484"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..577
                     /locus_tag="PLB86_07170"
     CDS             92..577
                     /locus_tag="PLB86_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64955.1"
                     /translation="MSFFDADQLQAYLRLSNDDSWDDVTIIAASARQAVEDRCGAVDI
                     RDVSELMTSAALTYGPVIELLSVSRDGAALSVADYRVGRGDVLAALSGSSVAGLTVTY
                     RVGRNPVPEWAGMAARIIARHLWRTQRPSRAGSDEQTSGFAIPNAAASLMAPHATFGI
                     A"
     gene            574..1032
                     /locus_tag="PLB86_07175"
     CDS             574..1032
                     /locus_tag="PLB86_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64956.1"
                     /translation="MIETSSRLPEFVEAIVAAVAPAIPDGLVLYGDPGDQALPDLVVS
                     VMWAPEDGDGATVRTERAEGLGHRYRDVYQVRCVVSIWSGDHYDTVASGMLRRCRDVM
                     DSVELALKQTQGLGVLDDAGLGPDIASGVRQVKDGTAVEVRFSVVGTALR"
     gene            1670..2098
                     /locus_tag="PLB86_07180"
     CDS             1670..2098
                     /locus_tag="PLB86_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64957.1"
                     /translation="MYFLPTIAAGNLTPTRPEITAGTDLSAEIADLAGWAVGVDMIDV
                     PDLGSRFTAQINGSIKPEQSSITFYGDVGGADARTNLPVDQAGFIVFMDGGDVPTKKL
                     DAYPVKVASHSKLRTLTNAFQITISFSITKAPGENLTIPA"
     gene            2095..2550
                     /locus_tag="PLB86_07185"
     CDS             2095..2550
                     /locus_tag="PLB86_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64958.1"
                     /translation="MTTLRDRLAAKSRRRVVVPVQVAEMSEEAAAELLDLQRTALAAA
                     QSGDMASLDGLNDRADSLREGTTVPVAFTATPAFDKVLGAYPDSEGGTDWLAALPMLA
                     GLCAEDESLQDDAVWVELLDSWSHGEKLTLWSALVGVNTVALSPHIPKG"
     gene            2908..>5659
                     /locus_tag="PLB86_07190"
     CDS             2908..>5659
                     /locus_tag="PLB86_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HRC64959.1"
                     /translation="MSTTDLLVRIESQFQDRGFKSAEASAKTLERELNKLEHAERAMA
                     NLQIQAAREDAVRTQTRLAAMEKVGRGATAMGVATAAGYALAAKAAMDWESAWTGVTK
                     TVDGSASDMAKLQSELRGLANTLPTTHEQIAAVAEAAGQLGVKRADVAAFTRTMIDLG
                     ETTNLTSDEAATGLAKLGNIMGVLPSQASRAGSALVALGNDGASTEQDILAMALRIGA
                     AGRTIGLTEAQVMGFASALSSVGIEAEAGGSAISRVFVDVAQAVDKGGVQVERFARVA
                     GMSVDSFSEKFRTDAAGAVTAFVEGLGRVEASGGSAFQTLEDLGLSEIRVRDTLLRAA
                     NASDLLRTSVDLGTKAWTDNLALTQEASKRYETAESKIKMARNQLNNAAIDVGAVVLP
                     AFASAAESVGSLAQWFSALPEPVKHTATVLGMVASAVTLVGGTALIAIPKIQAFKVVL
                     VEMGIASEASTARMGNFAKTAGILGAAIGATTVAGAGMKSVWLNTKGASDDAARSLEA
                     YIKGGAEAEHVTELMRNEFTGLGNAVNNVLNPSIWHAGAAGIDEWTTAYGLFGTSATT
                     DSIAFFRQLDVALTDFVKSGKGDQAAKVFGQITEEAKAQGYTLGQVQGLLPGYISSLD
                     QLSMSQGGLNKSVSPTVDTVEDYVQAATAATAATKAYADAINGFASPILDAREANRQW
                     EESIDSAAAALKENGKTLSDSTDKGRENAQALDGMTRAAIDNVGAMQANGESQGRLQQ
                     VIGESRAAIEAMAIRFGMSKDEARKYTDQLLTIPAVRATQISADTSRATAAIQGLFSY
                     WSSRTITLRVAAQADRVGTATGGVRSFPGGMMSYAEGGSFQGQVPRTAWNQGARGVQW
                     AETGAGAWEAWISGHPGRRQRSLAVHRDVGRKLGASAGIDPAALASAVRQALHGARLD
                     LGP"
     assembly_gap    5660..5759
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5882..7714
                     /locus_tag="PLB86_07195"
     CDS             5882..7714
                     /locus_tag="PLB86_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64960.1"
                     /translation="MSVSQRGYVALSQGAVGRSISWPAGTAAGDVAVLVLTDSDGRAP
                     ASGPSGWTFGRRYVWWRVLTAADVAAALSAPAVVVGVVVWAGAGVPSSSTRQSSVSVP
                     AGGAAFFAGVEWWGNNPGGATGRLGAVLTLSAIDVAVGMWGYTAAAAGVSGVSGASPD
                     ASWSGLVFPALAGPAVPTLVSPTSGQQVASAAAVALQWVHNSTVGGVQTAYRAQVRAV
                     GAGSWSYVAADGTLNASVQTVTSAAGSASINAGLLTAGTSHEWTVSTQEAGVWSAAAA
                     VGTFVPVASPSVSSVSVSSPLGTLAPAVSWSAAAGVGGLTAFQVLVTLSGGSPDAPLY
                     DSGVQVGAGSAWTVPARSDWTNGGSYKAWVRVAQAGGLWSPWVSGSFTVSWTAPAAPS
                     SVTVAQGSPPVVTIGSVPSGHGVGVQWSSDAGATWTTLATLAAGAAGSRSLAVPLAPF
                     GAGVLFRASSILTSGGVDLVSAWTTSAPFTSADEAAYLVSASDPAEYVRVSISSDGGR
                     SVIQSVAVWSPLGGARVRVDRTVPQGESGTTTLQTETDADTQTLLSWLTGDAFWLRWN
                     PETQAGVKLGMAPTLMAVADTPARERIAQIPIGWRRITFPWVQQ"
     assembly_gap    7880..7979
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7980..9780
                     /locus_tag="PLB86_07200"
     CDS             <7980..9780
                     /locus_tag="PLB86_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64961.1"
                     /translation="PGGSRSDMAISYVGANVYAGASAFIPTYQAGDLLIVAGWRSGSF
                     LANPSGWTLVVGATASNYYARAWSKVAASGSSSDMVTLTGASYIGVWVVRGASGVGAS
                     AVNVASTTYPGLTLQQTNGTSWVMGMGFGTTVRPATPAGMTSRSAASLVRASDTNGGV
                     TSRASGSTYGLGHHFAVEILAPPIALSMADTIPTGEAHYQPQWLHDGQILLRPDTIPT
                     AETLFAPTVKVATWRDWQDTPWWVGLVQSRVRTVGSRVELVDSTGAVVADLPVAAASV
                     ELHGTQPEQWAGSVVIRDPDWLPQDPTDLLDPRAGLRARIWWRLLTGVDSGPEVPLAT
                     LVLGDPQVRDREGAWEMTLTGRDVLSLPKRGGYRGLILDLGGMTVTAALKLLFAQLAP
                     TVPVRVADTTLTLPSGYQVGLSDRTPAQDWTDLADVAGWVVRSDRDGVIVAGPRVDRT
                     TPRADWSEGDDATVIELDRDVATSDTVNRVIVRSTSTAGVWAVVEDDDPGSATWVGRY
                     GPYERLIESDAVTTAEGALSMARMQYGRLRLPTESVPVRVPVRPDLDYRDPVILRRDR
                     SGVGGTYLVSGWTIRLGSSPSLMEVAMMPRDML"
     gene            9777..10187
                     /locus_tag="PLB86_07205"
     CDS             9777..10187
                     /locus_tag="PLB86_07205"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRC64962.1"
                     /translation="MSVRRVVERMTPRPAPRVILGTAAGEVSGGRVAVDLGGAVISAA
                     CAVPGVTAGTDVRLLVHGNGATVLHAQDPAGTLVPVGGIIAWPSGPAIPPGWITCTGT
                     YSAATWPRLYAVLGTTTLPSPTAPTGCVWIIRAT"
     gene            10194..10352
                     /locus_tag="PLB86_07210"
     CDS             10194..10352
                     /locus_tag="PLB86_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64963.1"
                     /translation="MTIPEILSGLRRLAEEFRALGLSEYARGRADLHGAWCDAARRLD
                     ELTDDLED"
     gene            10352..11137
                     /locus_tag="PLB86_07215"
     CDS             10352..11137
                     /locus_tag="PLB86_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64964.1"
                     /translation="MPERWIAEAEQLTPSGAPGTMSGVGPPRAICHVTVSRPGSFDAM
                     HRVLTDKMAEPHVLYDWATDRLGQYFALDRSARALRRGTRDVSCNKVGAACIQIEFCS
                     MPDGFTRYWTPGPNFRALMRAIASWGITPQFVARANTSAGDNVRQTWETYLATGGWWG
                     HCHVPENDHWDPGPIDQAAFFAAAGITTQEDDPMAMTPEDRTALAKEIGREVAQALLS
                     APIGTSAADAPADLGWGLYRIQSELMRLRGLVEQALESKAAQP"
     gene            11134..11466
                     /locus_tag="PLB86_07220"
     CDS             11134..11466
                     /locus_tag="PLB86_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64965.1"
                     /translation="MTLPEPTLAEVVRRLDGISTEIAGMRAELGTTYLRQDVHRVVHD
                     GITTALNAKADRETVEEVRKEVSAIQADMAAQEDFRRKIMLAVIGLGLTNGAALVVAV
                     VQRLAGGS"
     gene            11548..11676
                     /locus_tag="PLB86_07225"
     CDS             11548..11676
                     /locus_tag="PLB86_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64966.1"
                     /translation="MVEIALTAGFLFIMLAALAVLSMFITRAEARDRREHDTDHDL"
BASE COUNT         1702 a         3918 c         4101 g         1920 t
ORIGIN      
        1 atccaccaga ccacggccac ggcctgagca acgacaacgc cgcagggcgg gccgatccgg
       61 tccgccctgc ggcccgtctg gagggaacca cgtgtccttt ttcgacgccg accaactcca
      121 ggcgtacctg cggctgagca acgatgacag ctgggatgac gtcacgatca tcgccgcctc
      181 cgcccgtcag gccgtcgagg acagatgcgg agcggtcgac atccgggacg tctcggagct
      241 gatgacctcc gccgcgctca cgtacggccc tgtcatcgag ttgctgtcgg tctctcgcga
      301 cggggcggcc ttgtccgtgg ccgactaccg tgtcggccgg ggtgacgttc tggccgcgct
      361 gtcagggtcc agcgtcgctg gactgaccgt tacttatcgg gtcggtcgca atcccgtccc
      421 ggagtgggct ggcatggcgg cgcggatcat tgctcgccac ctctggcgga cgcagcgccc
      481 gagccgcgct ggctctgacg agcagacgtc cgggttcgcg atccccaatg ccgccgcgag
      541 tctcatggct ccgcacgcga cgtttggcat cgcatgatcg agacatcgag ccgcctcccc
      601 gagttcgtcg aggcgatcgt cgccgccgtg gctccagcca tcccggatgg gctggtgctc
      661 tacggggacc ctggggacca agcgcttccc gacctggttg tctccgtgat gtgggcgccc
      721 gaggatgggg atggcgcgac cgtccggacg gagcgggccg aggggctggg gcaccgttat
      781 cgcgacgtct accaggtgcg ctgtgtggtg tcgatctggt ctggggacca ttacgacacg
      841 gttgcgtctg gaatgctgcg gcgctgccgt gacgtcatgg attcggtgga gttggcgctg
      901 aagcagaccc aagggcttgg cgtgttggat gacgccggct tgggcccgga cattgcgtct
      961 ggggtgcgac aggtcaagga cggcacggca gtcgaggtcc ggttctcggt agtcgggacg
     1021 gcgctgcgct aatgcccgtc gaatacccac ggctcagtgt gctcgtaggc gaactcgggg
     1081 ttctgccgcg agcggtccgc tccgaactcc ggcggagctt tcgcgcggcc ggtgagaccg
     1141 cgctcacgaa ggcccgatca aatgcaggct ggtcgtctcg gatcccccaa gcaatcagcc
     1201 tgcgcacatc gacgcgtggc ccgcgtgctg gcgtggccct gcgagtggat gcccgagtcc
     1261 cacacgcccg cccatacgag ggcctgaggc gcggcggcgg ccgatccttc cggcacccgg
     1321 tctttgggga gggctgggtt atccaggcaa cgcgcccgta cctacgcccc gccgtcgatt
     1381 cagtctcgga ggccgtcctg gccgcctccc gagacgcagt ccagatagcc gcccgatccg
     1441 cgggcttccg ctgaggagac gaacacatgc cgctgtgcgc acaccctgac ctgcctggcc
     1501 aggtcgctga cctcatcaat cccgaggcat tcccgggatg ggaacccatc gagccccccg
     1561 ccgagtccga ccccacccct gagcctcgcg acagcgcggc cgagaccgag gagtaacaat
     1621 gcctgccctg actgccacca cgaggttctt ccagcgcggc accacgaagg tgtacttcct
     1681 gccgaccatc gccgcgggga acctgacccc tacccggccc gagatcacgg ccggtactga
     1741 cctgagtgcc gagatcgcag accttgcggg ctgggctgtc ggcgtcgaca tgatcgacgt
     1801 gccggacctc ggatcgcgct tcacggccca gatcaacggc tcgatcaagc ccgagcagtc
     1861 gagcatcacg ttctacggcg acgtgggcgg cgccgatgcg cggacgaacc tgcccgtcga
     1921 ccaggccggg ttcatcgtct tcatggatgg cggcgacgtc ccgacgaaga agctcgacgc
     1981 ctacccggtt aaggtcgcct cgcacagcaa gctgcgcacc ttgaccaacg cgttccagat
     2041 cacgatttcg ttcagcatca ccaaggcgcc gggcgagaac ctgacgatcc cggcgtgacg
     2101 accctacggg accgcttggc ggcgaagtct cgccgccgag tggtcgtccc ggtgcaggtg
     2161 gccgagatgt ccgaggaggc cgcagcggag ttgctggacc tccagcggac ggctcttgcc
     2221 gctgcgcagt cgggcgacat ggcgtcactg gatggcctca acgatcgggc ggacagcctg
     2281 cgcgaaggca cgaccgtgcc cgtggcgttc acggccacac cggcattcga caaggttctc
     2341 ggcgcgtacc ccgacagcga gggcggcacc gactggctcg cggccctgcc gatgctggcg
     2401 gggttgtgtg cagaggatga gtccttgcaa gacgacgccg tgtgggtcga gctgctcgac
     2461 tcgtggagtc acggggagaa gctcaccctg tggtctgcct tggtcggcgt caacaccgtg
     2521 gccctgtcgc cccacatccc aaaaggctga ggcgggaccg cctgttcgcg gcgcgcatgg
     2581 cgtactgcgg accccgcgga atcccgctcc atgaattcac cgcatggcct gaggagtcgc
     2641 aggcggcggc cctgcactgg caggagtacg aggcgtcgcg ttgcacgacg tgcgggacgt
     2701 tcgatgacga ctggatcgac gcgtccgggg agcctcagga gccgcagcac tggcacggcc
     2761 gggtctgccc tggctgccag cgactccagg ccgcacaaga gtccatgaat gccgagcagg
     2821 gcgaacgggg cctgcacgtc gccgccgcag acggtccagc cgctagctgc gttctctgtc
     2881 gcgccaacac atgaataggg ggtccgggtg agcactaccg acctgctcgt ccggatcgag
     2941 tcccagttcc aagaccgggg cttcaagtca gctgaggcgt ccgccaagac ccttgagcgg
     3001 gagctaaaca agcttgagca cgccgagcgg gctatggcca acctgcaaat ccaggcggcc
     3061 cgcgaggatg ccgtgcggac acagacgcgc ctcgccgcca tggagaaggt gggccgtggg
     3121 gctaccgcga tgggcgtcgc tacagccgcc ggctatgccc tcgcggccaa ggccgccatg
     3181 gattgggagt cggcctggac cggagttacc aagacggtcg acgggtccgc gtcggacatg
     3241 gcgaaactcc agagcgaact ccgcggcctg gccaacacgc tcccgaccac gcacgagcag
     3301 atcgcggcgg tggctgaggc tgccggtcag ctgggcgtga agcgcgctga cgtcgcggca
     3361 ttcacgcgga ccatgatcga cctcggggag accaccaacc tgacgtccga cgaggcggcc
     3421 actggcctgg cgaagctcgg caacatcatg ggggtcttgc cgtcacaggc atccagggcc
     3481 gggtcggcgc tggtcgccct cggtaacgat ggggcgtcga ccgagcaaga catcctcgcc
     3541 atggcgctgc gcatcggggc cgcaggtcgg accatcgggc tgactgaggc ccaggtgatg
     3601 gggttcgcct cggcgctgtc gtcggtcggc atcgaggccg aggcgggcgg ttccgcgatc
     3661 tcccgcgtgt tcgttgacgt cgctcaggcc gtcgacaagg gcggcgtgca ggtcgagcga
     3721 ttcgcgcgcg tcgctggtat gtccgtcgac tcgttctccg agaaattccg cacggacgcg
     3781 gcgggcgccg tgaccgcctt cgtggagggc ttgggccgag tcgaggcgtc tggcggatcg
     3841 gcattccaga ccctcgagga tcttggcctg tccgagatca gggtccggga caccctgctg
     3901 cgcgcagcga acgcgagcga cctgctgcgg accagtgtcg acctgggcac gaaggcatgg
     3961 accgacaacc tcgcgctgac ccaggaggcc agcaagcggt acgagacggc cgagtcgaag
     4021 atcaagatgg ctcggaacca actcaacaac gccgcgatcg atgtcggagc cgtcgtcctc
     4081 cctgcgttcg cgagcgcggc tgagtcggtc gggtcgctcg ctcagtggtt ctcggcccta
     4141 ccagagcccg ttaagcacac ggctacggtc ctggggatgg tcgcgtctgc ggtcacgctc
     4201 gtaggtggga ctgcgctcat cgcaatcccc aagatccaag ccttcaaggt cgtcctcgtc
     4261 gagatgggca tcgcgtccga agcgagcacg gcccggatgg ggaacttcgc caagaccgcc
     4321 ggcatcctcg gcgctgcgat cggtgccacc accgtcgccg gggccgggat gaagtccgtc
     4381 tggctcaaca ccaagggagc cagcgacgac gctgcccgat ccttggaggc ctacatcaag
     4441 ggcggggccg aagccgagca cgtcaccgag ttgatgcgca acgagttcac cggcctgggc
     4501 aacgccgtga acaacgtcct gaacccgtct atctggcacg ccggggccgc gggcatcgac
     4561 gaatggacca ccgcctacgg gctgttcggc accagcgcca ccaccgactc catcgcgttc
     4621 ttccgacagc tcgacgtcgc cctgaccgac ttcgtgaagt ccgggaaggg tgatcaggcg
     4681 gccaaggtgt tcggccagat caccgaggaa gccaaagcgc agggttacac cctgggccag
     4741 gtccaaggcc tgctccccgg gtacatctcc tcactcgatc agctcagcat gtcccaaggc
     4801 gggctcaaca agtcggtctc cccgaccgta gacaccgttg aggactatgt ccaggccgcg
     4861 acggccgcga cggccgcgac gaaggcgtat gccgacgcga tcaacggctt cgcgtctccg
     4921 atcctcgatg cgcgcgaggc aaaccggcag tgggaggagt cgatcgattc cgccgccgcc
     4981 gcgctcaagg agaacggaaa gacgctctcc gacagcaccg acaagggccg cgagaacgct
     5041 caggcgctcg acgggatgac ccgtgcggcg atcgacaacg ttggcgcgat gcaggcgaac
     5101 ggcgagagcc aggggcgact ccagcaagtc atcggtgagt cgcgcgccgc tatcgaggcc
     5161 atggcgatcc ggttcggcat gtccaaggac gaggcgcgga aatacaccga ccaactcctg
     5221 acgatcccgg ccgtccgcgc cacccagatc agcgccgata cgtccagggc tacggccgcg
     5281 atccaggggc tgttcagcta ctggtcgagc cggaccatca cgctgcgcgt cgcagcccag
     5341 gctgatcgcg tcggcaccgc cactggcggg gtgcgctcgt tccctggcgg gatgatgtcc
     5401 tacgccgagg gcgggtcgtt ccagggccaa gtgcctcgga ccgcctggaa ccaaggggct
     5461 cgcggcgtgc agtgggccga gactggcgcg ggcgcgtggg aggcgtggat cagcggacac
     5521 ccggggcgcc gtcaacgatc cctcgcagtg caccgggatg tcgggcgcaa gctcggcgcg
     5581 tccgctggca ttgacccggc cgcattggcc tcagccgtcc gtcaagctct gcatggcgct
     5641 cgcctggatt tgggtccggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     5761 tggcctcagc cgtccgtcaa gctctgcatg gcgctcgcct ggatttgggt ccggtgtcgg
     5821 cgatcactga ccgggtgtgg gcgacattgg atctggcgca gtctcgggag gcggtctatt
     5881 cgtgagcgtc tctcagcggg ggtatgtcgc cctgtcgcag ggtgcggtcg gtcggtcgat
     5941 ttcgtggcct gcgggcacgg ccgctgggga tgtcgcggtg ttggtgttga cggactccga
     6001 tgggcgggct ccggcgtcgg gtccgtccgg gtggacattc ggtcgccgtt acgtgtggtg
     6061 gcgggtattg accgctgcgg atgtggcggc cgcgctgtcg gcgcctgccg tggtggtggg
     6121 tgtggtggtg tgggctggtg ctggggttcc gtcgtcgtcg acgcggcagt cgtcggtgag
     6181 tgtcccggct ggtggtgctg cgttcttcgc gggcgtcgag tggtggggca acaacccggg
     6241 tggggcgacg ggtcgcctcg gtgccgtgtt gaccttgtct gcgattgatg tcgcggtcgg
     6301 catgtggggg tacaccgcgg cggcggctgg ggtgtcgggg gtgtcgggtg cttccccgga
     6361 tgcgtcatgg tcggggctgg tgttcccggc gttggcgggg cctgcggtgc cgactctggt
     6421 gtccccgacg tcggggcagc aggtggcgtc cgcggcggct gtcgcgttgc agtgggtgca
     6481 taactcgacg gtgggtggtg tccagacggc ataccgggcg caggtccgtg cggtgggtgc
     6541 cgggtcgtgg tcgtatgtcg ccgctgatgg gaccctcaac gcgtcggtgc agaccgtcac
     6601 ctcggccgct ggatcggcgt cgatcaacgc gggcctgctg acggcgggca cgtcgcatga
     6661 gtggacggtg tccacccagg aggcgggcgt ctggtccgct gcggccgcgg tgggaacgtt
     6721 cgtgccggtc gcgagcccgt ccgtgtcgtc ggtgagtgtg tcgtccccgt tggggacgtt
     6781 ggctccggcg gtgtcgtggt cggctgcggc tggcgttggc gggctgacgg cgttccaggt
     6841 gctcgtcacc ctctctggtg ggtccccgga tgcgccgctg tatgactcgg gggtccaggt
     6901 cggtgcgggg tcggcatgga ctgtcccggc ccggtccgac tggaccaacg gtgggtccta
     6961 caaggcgtgg gtgcgggtcg cacaggctgg cggcttgtgg tcgccgtggg tgtcggggtc
     7021 gttcaccgtc tcctggacgg ccccggctgc cccgtcatcg gtgacggtgg cgcaaggctc
     7081 accaccggtc gtgacgatcg ggtccgtgcc gtcggggcat ggggtgggtg tgcagtggtc
     7141 atccgacgcg ggggccacgt ggacgacgtt ggcgactttg gcggcaggtg cggcggggtc
     7201 gaggagtttg gcggtgccgt tggcgccgtt cggggcgggc gtgttgttcc gggccagctc
     7261 gatcctcacc tctggcgggg tggacctggt gtcggcgtgg accacgtcgg ccccgttcac
     7321 gtctgccgat gaggccgcgt atctggtgtc cgccagcgac ccggccgagt atgtgcgggt
     7381 gtcgatcagc tccgacgggg gccggtcggt catccagtcg gtggcggtgt ggtcgccgct
     7441 gggcggggcc cgggtgcggg tggatcgcac cgtcccgcaa ggggagtcgg gcaccaccac
     7501 cttgcagacc gagaccgacg cggacacgca aaccctcctg tcctggctga ccggggatgc
     7561 gttctggctg cgatggaatc cggagaccca agcgggcgtg aagttgggga tggccccgac
     7621 gttgatggct gtcgccgaca ccccggcccg ggagcggatc gcccagatcc cgatcgggtg
     7681 gcgccggatc accttcccct gggtccagca atgacgttgc gaaagaggcc gattcgatga
     7741 gtgccctata cccttccgcc cgccagggat tcctcacggg cctgatcgac gctgacaccg
     7801 cggtcatcaa ggctgccctc gtgcgcggct acacctacaa cgctgggcac gtctacatgt
     7861 cggatgtgac tgccgcgggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     7981 ccgggtggtt cacgctctga catggcgatc tcctatgtcg gcgcgaacgt ctatgcgggt
     8041 gcgtcggctt tcatcccgac ctatcaggct ggggacctgc tgatcgtcgc cggatggcgg
     8101 tcggggtcat tcttggccaa cccgtccggg tggacgcttg tggtcggagc gaccgcaagc
     8161 aactattacg ccagggcctg gtccaaggtc gccgcgtctg ggtcgtcgtc tgatatggtc
     8221 accctgaccg gagcgtcgta catcggcgtg tgggtggtcc gtggggcctc aggtgtgggc
     8281 gcgtccgcgg tgaatgtggc ctccacgacc taccccgggc tgacgttgca gcagaccaat
     8341 ggcacgtcct gggtcatggg tatgggcttc gggacgaccg tccggccagc gaccccggcg
     8401 gggatgacat cccggtcggc cgcgtcgctg gtccgcgcct ccgacaccaa cggcggagtg
     8461 acctcgcggg cgtccgggtc aacatacggg ctcggccacc atttcgcggt cgagatcctg
     8521 gccccgccca tcgccctgtc gatggccgac acgatcccca ccggggaggc ccactaccag
     8581 ccccagtggc tgcacgacgg gcagatcctg ctgcgccccg acacgatccc caccgccgag
     8641 acgttgttcg ccccgaccgt caaggtagcg acgtggcggg actggcagga caccccctgg
     8701 tgggtgggct tggtgcagtc ccgtgtccgc acggtcgggt cccgggtcga gctcgtcgac
     8761 tccactggcg cggtcgtcgc cgacctgccc gtcgcggccg cgtccgtcga gctgcacggc
     8821 acccaaccgg agcagtgggc ggggtcggtg gtgatccgcg accctgactg gctgccgcag
     8881 gaccccaccg acctattgga cccgcgggct gggctacggg cccggatctg gtggcggctg
     8941 ctgaccggcg tcgactccgg ccctgaggtg ccgttggcga ccctcgtgct gggggacccg
     9001 caggtccgtg accgtgaggg cgcctgggag atgaccctca ccggccggga cgtcctgtcc
     9061 ttgcccaagc gtggcggata ccgcgggctg atcctggact tgggtggtat gaccgtcacc
     9121 gcggcgctca agctgttgtt cgcccagctg gctccgaccg tgccggtccg ggtcgcggac
     9181 accaccctca ccctgccgtc cgggtatcag gtcgggttgt cggaccggac ccccgcgcag
     9241 gactggacag acctggccga cgtggccggg tgggtggtcc gctccgaccg ggacggggtg
     9301 atcgtcgcgg gcccccgcgt ggatcgcacc accccacggg ctgactggag cgagggcgac
     9361 gacgcgacgg tgatcgagct ggaccgcgac gtggccacct ccgacaccgt caaccgggtc
     9421 atcgtccgat ccacctccac cgccggggtg tgggctgtcg tcgaggatga tgaccccggc
     9481 tcggcgacgt gggtcggacg ttacgggccg tacgaacggc tgatcgagtc cgacgcggtc
     9541 accactgctg agggcgcgct gtcgatggcc cggatgcagt acgggcggct acggctgccc
     9601 accgagtcag taccggtgcg ggtgccggtc cgtcccgacc tggactaccg cgatccggtg
     9661 atcctgcgcc gagaccggtc cggggttggc ggcacctacc tggtgtccgg gtggacgatc
     9721 cgcctcggat cgtcaccatc gctgatggag gtcgccatga tgcctcggga catgctgtga
     9781 gcgtccgccg cgtcgtcgag cggatgactc cccgcccggc accgcgggtc atcctcggca
     9841 ctgccgcggg cgaggtgtcc ggcgggcggg tcgccgtcga cctggggggc gcggtcatct
     9901 ctgccgcgtg cgccgtccca ggggtcaccg cgggcacgga tgtgcggctg ctggtccacg
     9961 gcaatggcgc gacagtcctg cacgcccaag accctgccgg caccctcgtg ccggtcggcg
    10021 ggatcatcgc ttggccttcc ggtcccgcga tccctcccgg gtggatcacc tgcaccggca
    10081 cctactccgc cgcgacgtgg ccgcgcctgt atgccgtgct cggcacgacc accctcccat
    10141 cccccaccgc tcccaccggg tgcgtgtgga tcatccgcgc cacctgagga gccatgacga
    10201 tccctgagat cctctccggt ctgcgtcgcc tcgctgagga gttccgggcg ctcgggctct
    10261 ccgaatacgc ccgcggccgc gccgacctac atggcgcctg gtgcgacgcc gcgcgccgcc
    10321 tggacgagct caccgacgac ctggaggact gatgcctgag cgatggatag ccgaggctga
    10381 gcaactcacc ccgtccggcg cgccgggcac catgtccggc gtcggtccgc cgcgggccat
    10441 ctgccacgtc accgtctccc ggcccggatc tttcgatgcc atgcatcgag tcctgacgga
    10501 caagatggcc gaaccgcacg tgctctacga ctgggccacg gaccgactcg ggcagtactt
    10561 cgcgctggac cgttcggccc gagcgcttcg acgcggcacc cgagatgtct cgtgcaacaa
    10621 ggtcggggcg gcgtgtattc agatcgaatt ctgttccatg ccagacggat tcacgcggta
    10681 ctggactccc ggaccgaact tccgggccct catgcgcgcc atcgcgtcgt gggggatcac
    10741 gccgcagttc gtggcccgcg ccaacacgag cgctggtgac aacgtgcgcc agacgtggga
    10801 gacgtacctc gccacgggcg gctggtgggg tcactgccac gtccccgaga acgaccactg
    10861 ggacccgggt cccatcgacc aggccgcctt cttcgccgcc gcaggcatca ccactcagga
    10921 ggacgacccc atggccatga ccccagaaga ccggaccgcg ctcgccaagg agatcggccg
    10981 cgaggtcgct caagccctgc tgtccgcccc catcggcacc agcgccgcag acgcgcccgc
    11041 cgacctcggg tggggcctct accgcatcca gtccgagctg atgcggctgc gcggcctcgt
    11101 ggagcaggcg ctcgagagca aggccgctca gccgtgacgc tgcctgagcc gaccctcgcg
    11161 gaggtagttc gccgcctcga cggcatctcc actgagattg ccgggatgcg agccgaactt
    11221 ggcacgacat acctgcgtca ggacgtccat cgcgtcgtcc atgacgggat caccaccgcg
    11281 ctgaacgcca aggccgaccg ggagacggtc gaggaggtcc gcaaagaggt ctccgcgatc
    11341 caagcggaca tggccgccca ggaggacttc cgccgcaaaa tcatgctcgc ggtcatcgga
    11401 ctcggcctca ccaacggcgc ggccctggtc gtcgccgtcg tgcagcgcct ggccggtggc
    11461 tcatgatccg cgacgccgcc tgggctgccc tgtcggccgt cctcgcgggc ggcatctggg
    11521 tggtcctcat cgcgcggcgg gtatgccgtg gttgagatcg ccctcaccgc cgggtttctc
    11581 ttcatcatgc tcgccgccct cgccgtcctc tccatgttca tcacgcgcgc cgaagcccgc
    11641 gaccgccgcg agcacgacac cgaccacgac ctctaggagc accccatgaa ccccctctcc
    11701 gaacgccccg atatccgccg ccgcgtctac atcgcgttct gggggttggg cctcacgctc
    11761 ggcgccgccc agaccgggta tgccgccgcg caggccggac agcccgtgtg gctcaacgtc
    11821 gcgctgagcg tctacgcctt c
//
LOCUS       DAORPW010000070        11791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1177_length_11791_cov_637.595688, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000070 DAORPW010000000
VERSION     DAORPW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11791
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1177_length_11791_cov_637.595688"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..842)
                     /locus_tag="PLB86_07230"
     CDS             complement(48..842)
                     /locus_tag="PLB86_07230"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRC64967.1"
                     /translation="MVQIIDRAGWGFTGWTSPRTVVPVGERTVFEVHYDGGAPVGHIV
                     GTAAPQAIDAEHKGNGWAGIGYSFIVDQLGNIFEGRGWDFQGSFCPVHNRTAWAVQVH
                     IGGDEQPSPQALAAVRGLYDEACARRGLALTPIGHRDGYATDCPGRALYAWVHTGMPI
                     TQEDPDMNPWNTVLGPGYTGGKNQVGPAEAQVWVVNTLCALYDLLAAAGRIEAAIGRI
                     EATVATVGTPELPADLSKTIGAAVSSGMADVVVDHLASRLARPSAT"
     gene            complement(860..1576)
                     /locus_tag="PLB86_07235"
     CDS             complement(860..1576)
                     /locus_tag="PLB86_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64968.1"
                     /translation="MDIGAPPEGWTYWDVGGQVNDGAAVVTLYDSAPANQGQYAALDC
                     FLNRLQLTAYSRSGLGAGWSFIPLDVRWDSAGNATLEMRNNAVDPAKRGVVALDVGSG
                     GRVLSGLTRLISESVQAGQLQLIGAKLLWGQLNLPWANGWSNFGGRWRTIDASKDQWG
                     VVRLRGLITNANAAATGNITTLPAGWIPADSEMFSCVAGPGGARVDVERDGTVNIVNY
                     FGGGNANYLSLHGISFQSDI"
     gene            complement(1639..2004)
                     /locus_tag="PLB86_07240"
     CDS             complement(1639..2004)
                     /locus_tag="PLB86_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64969.1"
                     /translation="MSGIYIQPAPEPSNSAGLARPGVATLTSQVDYDGRLAALWTPPG
                     GGDAWITRIRVNCDVACTAWVYFNSRTPANLITGTYAGGLDEYDAAQPIWVPDGTKVL
                     IVWDTQRAVSAHARIEYVR"
     gene            complement(2450..2725)
                     /locus_tag="PLB86_07245"
     CDS             complement(2450..2725)
                     /locus_tag="PLB86_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64970.1"
                     /translation="MTVVLPAVAEWGALAILAAVLGLCLRLLVEQRKELTRLADRVRE
                     LERHDAMHERRIDAHEPWDEQARERIRQLDPAHPLPIPPPLRRERRA"
     gene            complement(2731..3156)
                     /locus_tag="PLB86_07250"
     CDS             complement(2731..3156)
                     /locus_tag="PLB86_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64971.1"
                     /translation="MRKETVVRIPETLVAAVQALTYTVENTAERFAAEMEGTRNAVRI
                     SGCEARAIVDGAGVGLRVTTSPGRLAGWSVRETSGAAPAIVRFLDGPGGDGVAEIALP
                     AGQGSTLWLLPGGVSLTYGLSVQVVQGAVSGAVYVAGGG"
     gene            complement(3153..4139)
                     /locus_tag="PLB86_07255"
     CDS             complement(3153..4139)
                     /locus_tag="PLB86_07255"
                     /inference="COORDINATES: protein motif:HMM:NF039494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRC64972.1"
                     /translation="MALSPAAVFALARSAGFDASTAVIMTAIAGGESGWNPDAVGDVG
                     LQDGTWGPSVGLWQIRSIKAQNGTGGVRDASRLKDPAFNAASAYNIYKSQGLRAWSVY
                     SSGAYKNYLTRAQGATSSGSPAGGGGDAAVAFARAQIGKPYRWGATGPDAWDCSGLAQ
                     GSWKAAGVSIPRTTYQQILAGSSISTDQLAPGDLVFPDAGHVQIYAGGGRIIEAPRTG
                     VPVRDGALGKVMAARRVGTAGTNATNASLASVSGQLVELPGGNWNPLNWPGSILGNTA
                     ASTSQALWAQIQPFLLTGLFVSAGLGLVVIGTGITAWPSVRRGASAVTELTG"
     gene            complement(4140..4733)
                     /locus_tag="PLB86_07260"
     CDS             complement(4140..4733)
                     /locus_tag="PLB86_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC64973.1"
                     /translation="MNLAQVKAWGGSHRTALLGGSAGAVALVAVARRRKTTAVPAPAT
                     DTSAARMAGQSFTGAASVGGVGMDTSATDVYNALQPQIEANQSLLQKVWDKVTGIPEM
                     VNQAVTAADITGQVTNAIKATPIPVAPAPAPAPTPAPPAPAAPEPRTYTVVRGDNLSR
                     IAQRYYGTADWNRIYEANRGIITNPNLIYPGQTLVIP"
     gene            complement(4730..5074)
                     /locus_tag="PLB86_07265"
     CDS             complement(4730..5074)
                     /locus_tag="PLB86_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64974.1"
                     /translation="MATPAAINAIPQLVTTYGPDPRGIDLHTRLAGVLQRGIRGCTGL
                     AYVGDRGAPERSWNGDLGRSPQRFNGAAALGAGMPVHVHTGEIASSYVSGGITDPAMR
                     VFAARARRQANR"
     gene            complement(5078..5218)
                     /locus_tag="PLB86_07270"
     CDS             complement(5078..5218)
                     /locus_tag="PLB86_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64975.1"
                     /translation="MVRKLAGYGFGLIALYLVVVNGTNAGKLIKDGATGSSDLVKAFQ
                     GR"
     gene            complement(5231..5512)
                     /locus_tag="PLB86_07275"
     CDS             complement(5231..5512)
                     /locus_tag="PLB86_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64976.1"
                     /translation="MRTGVSLIGTVLTLTLLRAVLNSDATAGQLGAAGTTIAAIVRRW
                     VDPALPAIPDLRPGAQRSGTPSLDASGQAAPTLLPNRLPSPTAPDTIPA"
     gene            complement(5612..6076)
                     /locus_tag="PLB86_07280"
     CDS             complement(5612..6076)
                     /locus_tag="PLB86_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64977.1"
                     /translation="MSLAVAVKAAAAGKAGGGTRARTPHSRAAVGVARQHVKDAKAGT
                     SVDALRADLTRRGFAPETIDAATAATPAPAQVSPGRAETTAPARPGPQPSRTFGGTGS
                     GILLGLIAYPVLLAYINGGAAKVRQWFAAKWLNKGVGQPESMPTGGSSSGTW"
     gene            complement(6092..6427)
                     /locus_tag="PLB86_07285"
     CDS             complement(6092..6427)
                     /locus_tag="PLB86_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64978.1"
                     /translation="MTQPPGLDAYTSTANPAANPAYAQAGPLAGAANAPEQPPAPTPG
                     PSVAAPVPAAGTIVAHSRHDVNGWPSQALGIVVGVQDTLLGDVVVERVALVCALGEPT
                     RIPLDALTT"
     gene            complement(6424..6660)
                     /locus_tag="PLB86_07290"
     CDS             complement(6424..6660)
                     /locus_tag="PLB86_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64979.1"
                     /translation="MSYVSPSFGPGRLGPPSGSTPAPTETRTLHAPGTPDGVRDNPLV
                     PFAVLAAIAFGLMAFSTTVRVGKTRAAVQIGDTK"
     gene            complement(6675..7862)
                     /locus_tag="PLB86_07295"
     CDS             complement(6675..7862)
                     /locus_tag="PLB86_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64980.1"
                     /translation="MPAPIATTPLGQTQSMQGILVPASSVRPEEFFHRTRRHTLLESA
                     RPYAMSAAAGQDVFELRKSDILSRLLVRFTGSLVITTPTGTAASTARWPYDMLNAKFT
                     ANGASNVINASGLKLKARDAMKRGELSDRGVSQTVGGAAKTQGTLAMATESWGVGSNS
                     TLSAGTYPVDLMWIVPVAEDEIDLVGAIFLATSTADLTLTLDYAPLASLFTLTGTATA
                     ALTGTMQVTSTKFSVPIGSDGQIVVPDLSTFHSLIQSRTTALQNGENEIRIVGQGGGK
                     SLLRTFYQVWNGAVSAPLAMTTANYGKQSWRYGNNETPDEFIDGSTLRYENERLYNTD
                     LGGIWGFGCHEFASELAFRDVVDMGTTSDLRIISNIAAGVTLTNPAIEYVTETVFQAG
                     TAA"
     gene            complement(7862..8014)
                     /locus_tag="PLB86_07300"
     CDS             complement(7862..8014)
                     /locus_tag="PLB86_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64981.1"
                     /translation="MKRAQLPAFWLAVGGVSLIAPVLFNIAADRLGNAVPGLRTLNDY
                     VTRRNG"
     gene            complement(8246..8551)
                     /locus_tag="PLB86_07305"
     CDS             complement(8246..8551)
                     /locus_tag="PLB86_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64982.1"
                     /translation="MPYPPTFDRETIMPSTAFTADDRDFGARQLAKAHEIAAEPWNYG
                     ATDLEHAFITLHSSLAEHPYSLPFEASWQILDAPRREYLATFKSWVMLLGYRMTLSR"
     gene            complement(8555..9241)
                     /locus_tag="PLB86_07310"
     CDS             complement(8555..9241)
                     /locus_tag="PLB86_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64983.1"
                     /translation="MTDDVAEGSGWPARLDPQRNWIVSAFGRKGSGKTTFNRELYRDY
                     PYAKLCIDVNGEADPGPDAKRIREIDQKMPGPDRDGKPANLHWVADPGSSTYAVDLDR
                     AIGMAMYPSAVQVLVWAGEVGEFTTGNRTGPHLRRLLMQSRHYRTSALFDGPRPIDID
                     KLVIAQSDIVAVFDLPDPDDRVRVAKVIGYPPARFAIECDETFRRGDYWFLLWVTATK
                     RLYRCPPLPI"
     gene            complement(9238..9870)
                     /locus_tag="PLB86_07315"
     CDS             complement(9238..9870)
                     /locus_tag="PLB86_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64984.1"
                     /translation="MTTPSQDQDQPARARVGRPRFSSPPAPDPILDQLDATDATSPSE
                     SPNLDTRVDDGAPAPDAEPTPSPKGKSRRGSSRASSPGDAETLRHGIAAAVSGASVVV
                     HEVLARDDYDKVAGVWLADPQDVDGIADPAASIMSRRTGDLGAGNPDVADGISLAIAL
                     VFWLVKQMHKIRFARSMRRSGAAVDGFQPEVAPPTPPTNVPSAYDTAPPA"
     gene            complement(10632..11105)
                     /locus_tag="PLB86_07320"
     CDS             complement(10632..11105)
                     /locus_tag="PLB86_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRC64985.1"
                     /translation="MSAAAFATVGRDHAAPARLPGSGALAKQIRDGATVRQLAEDYGV
                     TSNRVRLVVYRGGWSAATGLPRVDRPKDPMPGVKKPPEPDRSWMDRAACNQMPDFTDR
                     DQREAARVCNGCPVVADCLAYAQALRIHHFVWGGLTDQQRAQLRRRTLRKRSETL"
     gene            complement(11102..11317)
                     /locus_tag="PLB86_07325"
     CDS             complement(11102..11317)
                     /locus_tag="PLB86_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC64986.1"
                     /translation="MPAEVLPDLVPKIAAAAAIARDAEDGWRLAVRLRDQFILAAVDA
                     GLSHREIAAAAGVTKSRIVGIILGAGA"
BASE COUNT         1678 a         3855 c         4287 g         1971 t
ORIGIN      
        1 acctgttata gcaggaggcg aggggccggc accacccccc aaatgggcta tgtcgcggat
       61 ggccgcgcga gccggctcgc caggtggtcc acgaccacgt cggccatgcc agaactcacg
      121 gcggcaccga tggtcttgga taggtccgcg ggcagctccg gcgtgccaac cgtggcgacg
      181 gtcgcctcga tgcgcccaat cgcggcctcg atgcgcccgg ccgcggcgag gaggtcgtac
      241 agggcgcaca gtgtgttcac gacccacacc tgagcctcgg ccggaccgac ttggttctta
      301 cctccggtgt agccggggcc aagcacggtg ttccatgggt tcatatcggg atcctcctgg
      361 gtgatgggca tgccggtgtg cacccacgcg tagagggcgc gtccggggca gtccgtggcg
      421 tagccgtcac ggtggccgat gggagtcagc gccaggccgc gcctggcgca cgcctcgtca
      481 tagaggccgc gcactgcggc cagagcttgt ggcgatggtt gctcatcgcc gccgatgtgg
      541 acctgtacag cccaggcggt gcggttgtgg acgggacaga acgacccttg gaagtcccat
      601 cctcgaccct cgaagatgtt gccgagctgg tccacaatga agctgtatcc gatgccggcc
      661 cacccgttgc ccttgtgctc ggcgtcgatg gcctgagggg cggcggttcc gacgatgtga
      721 ccgactggcg cgccgccgtc gtagtggacc tcgaagaccg tccgctcgcc gacgggcacg
      781 acggttcgcg ggctggtcca gccagtaaac ccccacccgg cccggtcgat gatctgcacc
      841 acggctttgc cctccctcgc tagatgtcgg attgaaagct gatcccgtgc agggacaggt
      901 agttggcgtt tcccccgccg aagtagttca cgatgttgac cgtcccgtca cgctcgacat
      961 cgacacgcgc cccaccgggc cccgcgacac atgagaacat ttccgagtcg gctggtatcc
     1021 agccggccgg aagggtcgtg atgttgccgg ttgccgccgc gttcgcgttc gtgatgagcc
     1081 cccgcaggcg caccaccccc cactgatcct tcgaggcgtc gatggtcctc cagcgcccgc
     1141 cgaagttgga ccatccgtta gcccatggaa ggttgagctg cccccacagc agctttgccc
     1201 cgatgagttg caactgaccg gcctggacgg attcggagat cagccgtgtc agtccactga
     1261 ggacgcgccc gccactgcca acatcaaggg cgacgacccc cctcttggca gggtctacgg
     1321 cgttgttgcg catctctagg gtggcattcc ccgcactgtc ccatcgcaca tcgagcggga
     1381 tgaacgacca gcccgcgccg agcccactcc gcgagtaggc cgtgagttga aggcggttga
     1441 ggaagcaatc gagcgcggca tactggccct ggttggccgg cgccgagtcg tagagggtga
     1501 caacagccgc gccgtcgttg acctgtccac cgacgtccca atacgtccac ccctcggggg
     1561 gcgcgccgat gtccacgcca gggctccctg gcgacgtgat cgtgcgtaca aggcgagtga
     1621 tcggattgtg ccaacccatt agcgcacgta ctcgatccgc gcgtgcgcac tgacggcgcg
     1681 ctgcgtgtcc cacacgatga ggacctttgt cccatcggga acccatatgg gttgggcggc
     1741 gtcgtactcg tccagcccgc cagcgtaggt gccggtgatg agattagccg gcgtgcgcga
     1801 gttgaagtag acccacgcgg tgcaagcgac atcgcagttg accctgatcc gagtgatcca
     1861 cgcatcgccg ccgcctggcg gggtccagag cgcggccagg cggccgtcat agtcgacctg
     1921 agaggtcagc gtggcaacgc cagggcgggc cagccccgca ctgttggagg gctcgggtgc
     1981 cggctgtatg tagatcccgc tcacgtcgcg caggtcctac gcgacggttg ccagacgcac
     2041 caggaagggg cgcgtctggc cccgaccctc atacgcctga tagatgagcc aggagccggc
     2101 cgcgaggagt cctagtccac cccatagggc ggttcggcga tccgtcacga ctggccgacg
     2161 gcgactcatc gcgcaccccc tgccgagccg tcgcccgtca ccacggcgga cgggatcgtc
     2221 gcggaaaggt cgccgggtga tggcctgctg gccgcgggga gcgacgtgtc gccttgatct
     2281 cggggcgacg gcacgaggaa tcgagtgttc gggagcatcg caccgccacc agctccgacc
     2341 gccgtcaggc cgattgcggt cagcagctcg ggcaccggga cgccaagaac cgcgcagatg
     2401 gtgacggcca ctacgacaag gccggccagg aggaaggcgt agacgggatt catgctcggc
     2461 gttcccttcg cagtggtggg gggatgggta gagggtgggc ggggtcgagc tggcggatcc
     2521 gttctcgggc ctgctcgtcc catggctcgt gggcgtcgat gcgccgctcg tgcatcgcgt
     2581 cgtggcgttc cagctcacgc acccggtcgg ccagtcgcgt cagttccttg cgctgttcca
     2641 ccagtagccg caggcagagc ccgagaacgg ccgcgaggat cgccagggcg ccccattcgg
     2701 cgaccgcggg caggacaacg gtcatgaggg tcacccgccg ccagcgacgt acaccgcgcc
     2761 agagacggcc ccctggacga cctggacgct cagcccatag gtgaggctga ctccacccgg
     2821 aagtagccac agcgtggagc cctggcccgc cgggagggcg atttcggcga cgccgtcccc
     2881 gccggggccg tcgaggaagc gcacgatggc cggcgctgct ccgctggtct ctcggaccga
     2941 ccacccggcc aggcggccgg ggctggttgt gacccgcagg cccacgcccg cgccgtcgac
     3001 tatggcgcgg gcctcgcagc cggagatccg caccgcgttg cgagtgcctt ccatttccgc
     3061 ggcgaaccgc tcggccgtgt tctccacggt gtaggtcagc gcctggacgg cggcgacaag
     3121 cgtctcgggg atccgcacga cggtttcctt tctcatccgg tcagctcggt gacggcgctg
     3181 gcgcctcggc gcacgctcgg ccaggcggtg atccccgtgc cgatcacgac aaggccaagg
     3241 ccggcgctca caaacagacc cgtgagcagg aagggctgta tctgtgccca aagggcctgt
     3301 gatgtggacg cggcggtatt gcccaggatc gacccgggcc agttgagcgg gttccagttg
     3361 ccccctggca gctccacgag ttggcccgag acggaggcga gggaggcgtt cgtcgcgttc
     3421 gtgccggcag tgccgacgcg ccgcgcggcc atgaccttcc cgagagcgcc atcacggaca
     3481 ggaacaccgg ttcggggcgc ctcgatgatc cttccgccgc cggcatagat ctgcacgtgg
     3541 cccgcgtcag ggaacacgag atccccgggt gcgagctggt ccgtgctgat cgagctgccg
     3601 gcgaggatct gctggtaggt ggtgcgcgga atgctgaccc ccgcggcctt ccagcttcct
     3661 tgcgccagac ccgagcagtc ccaggcgtcg gggccggtgg ctccccatcg gtagggcttg
     3721 ccgatctgcg ctcgcgcgaa cgcgaccgcc gcatcgccgc cgccgcctgc cggagaacct
     3781 gaggaggtgg ccccttgggc gcgggtgagg tagttcttgt aggccccgga gctgtagacg
     3841 ctccatgccc gaagtccttg gctcttgtag atgttgtagg cgctggcggc gttgaacgcc
     3901 ggatctttga gcctgctcgc atcacggacc ccgccggttc cgttctgtgc cttgatcgag
     3961 cggatttgcc acaggcccac ggatgggccc caggtgccat cttgcaggcc gacgtctccc
     4021 acggcgtcgg ggttccaccc gctctcaccg cctgcgatgg ccgtcatgat gacagcagtg
     4081 gaggcgtcga acccagctga gcgggccaac gcgaagacgg cagcggggga gagtgccatt
     4141 taggggatga cgagcgtctg gccggggtag atgaggttcg ggttggtgat gatgccgcga
     4201 ttggcctcgt agatgcggtt ccagtccgcc gtgccgtagt agcgctgggc gatccggctc
     4261 aggttgtccc cgcggaccac ggtgtaggtc ctcggttcgg gggcggccgg ggcgggtggc
     4321 gccggtgtgg gcgcaggcgc gggcgcaggc gccacaggga tgggggtcgc cttgatggcg
     4381 ttggtcacct ggcccgtgat gtccgcggcc gtgacggcct ggttgaccat ctcgggaatg
     4441 cccgtcacct tgtcccacac cttttgcagg aggctctgat ttgcctcgat ctgcggctgt
     4501 agggcgttgt agacgtccgt ggcgctcgtg tccataccca ccccgccgac actggccgcg
     4561 ccggtgaagc tctgaccggc catcctcgcc gcgctggtat ctgtggccgg cgccgggact
     4621 gctgtcgtct tgcgtcgacg cgcgacggca accagggcca ccgccccggc gctgccgccg
     4681 agcagggcgg tccggtggct accgccccac gccttgacct gggccaggtt catcggttgg
     4741 cctgtcgtcg cgccctcgcg gcgaagacgc gcatggcggg gtcggtgatc ccaccggaga
     4801 cgtacgagct ggcgatttcg ccggtgtgca cgtgcacggg catgccggcc cccagggcgg
     4861 cggcgccgtt gaagcgctgc ggggatcgcc cgaggtcccc gttccagctc cgctcgggtg
     4921 cgccccggtc tccgacgtac gccaggccgg tgcacccgcg aatgccgcgt tgaaggacgc
     4981 cggccaggcg cgtgtgtagg tcgattccgc gtggatcggg gccgtaggtc gtgacgagct
     5041 gggggattgc gttgatcgcg gccggggttg ccatggctta gcgcccctgg aacgccttga
     5101 cgaggtcgga gcttcccgtc gcgccgtcct tgatgagttt gcccgcgttc gtgccgttga
     5161 cgacgacgag gtagagggcg atgaggccga agccgtagcc ggccagtttg cgcaccatgg
     5221 gggggcctcc tcaggcgggg atggtgtcgg gagcggttgg gctgggcagg cggttgggca
     5281 gcaaggtcgg ggctgcctgt ccgctcgcgt ccagcgatgg cgtgcccgag cgctgggctc
     5341 ctgggcgcaa gtcggggata gctggcagtg cgggatcgac ccaccggcgc acgatggccg
     5401 cgatggtggt tccagcagct cccaactggc ccgcggtcgc gtcggagttg agcactgccc
     5461 gcaacagggt gagggtcagc acggtcccga tgaggctcac gccggtcctc atgccagcac
     5521 ccgcagccgg cgtagcgcgt ccccgccgat cacctggacg atgatccacg cgccgatcag
     5581 caccacggcg atcccggaag tgttcacgcg ctcaccacgt ccccgagctt gatccgccgg
     5641 tgggcatact ctccggttgc ccaacgccct tgttgagcca cttcgcggcg aaccattggc
     5701 ggaccttcgc cgcgccgcca ttgatgtagg cgagcagcac ggggtaagcg atgagaccga
     5761 gcaggatgcc cgagccggtg ccgccgaatg tgcgcgacgg ttggggcccg ggccgcgccg
     5821 gcgcggtggt ctcggcgcgg cctgggctga cctgagccgg cgcaggtgtc gcggcagtgg
     5881 ccgcgtcgat ggtctcgggc gcgaacccgc gtcgcgtgag atcggcgcgc agcgcgtcga
     5941 cgctcgtgcc ggccttggca tccttgacgt gctgcctcgc gacgccgacc gctgcgcggc
     6001 tgtggggggt gcgcgcgcga gtgccgccac cggccttacc tgcggcggcg gccttgacgg
     6061 cgacggccag gctcacggcg atgcggtcct ctcaggtcgt gagggcgtcg aggggaatgc
     6121 gcgtgggctc gccgagggcg cacacgagtg cgacccgctc gaccaccacg tcgccgagca
     6181 gcgtgtcctg cacgccgacg acgatgccca gggcctggga cggccacccg ttcacgtcgt
     6241 ggcgcgagtg ggcgacgatg gttccggccg ccgggactgg cgccgcgacg ctgggcccgg
     6301 gggtgggggc agggggctgc tcgggtgcgt tggccgcgcc ggccaggggg ccggcctgcg
     6361 cgtatgccgg attggcggcg ggattggcgg tcgaggtgta ggcgtcgagg ccgggcggct
     6421 gggtcacttc gtgtctccga tctggacggc cgcgcgggtc ttcccgacgc gtaccgtggt
     6481 gctgaaagcc ataagcccga acgcgatggc ggccaggacg gcgaacggga cgagcggatt
     6541 gtcccggact ccgtccgggg tgcccggggc gtgtagggtt cgggtctcgg tcggtgccgg
     6601 ggtcgatccg gacggcgggc cgagtcggcc ggggccgaag ctgggcgaca cgtaggacat
     6661 caggggtcct tcgatcaggc ggccgtgccg gcctggaaga ccgtctcggt cacgtactcg
     6721 atggccgggt tcgtcagggt gaccccggcc gcgatgttcg agatgatccg caggtccgac
     6781 gtggtgccca tgtcgaccac gtcacggaag gcgagttcgc tcgcgaactc gtggcagccg
     6841 aagccccaaa tgccgccgag gtcggtgttg tagagccgct cgttctcgta gcgcagggtc
     6901 gaaccgtcga tgaactcgtc cggcgtctcg ttgttgccgt agcgccacga ctgcttgccg
     6961 tagttcgccg tggtcattgc caggggagcg ctcaccgcgc cgttccacac ctggtagaac
     7021 gtccggagca ggctcttgcc cccgccctgt ccgacgatgc ggatctcgtt ctcgccgttc
     7081 tgtagcgccg tggtccgcga ttggatcagg gagtggaacg tcgagaggtc cgggacgaca
     7141 atctgcccgt ccgagccgat tgggacggag aacttcgtgc tcgtgacctg catggttccg
     7201 gtgagcgccg cagtggcggt gccggtgagg gtgaataggc tcgccagggg cgcgtagtcc
     7261 agcgtgaggg tgaggtcggc ggtcgaggtc gcgaggaaga tcgcgccgac aaggtcgatc
     7321 tcgtcttcgg cgaccggcac aatccacatg agatccaccg ggtatgttcc tgcggagagc
     7381 gtcgagttcg agcccacgcc ccagctttcg gtcgccatcg cgagggtgcc ttgggtcttg
     7441 gcggcgccgc cgaccgtctg agacacaccc cggtcgctca gctcgccgcg cttcatcgca
     7501 tccctggcct taagttttag gccgctcgcg ttgatgacgt tgctcgcgcc gttcgcggtg
     7561 aacttcgcgt tcaacatgtc gtagggccac cgcgccgtgc tggccgcagt gccggttggg
     7621 gtcgtgatga ccagcgaccc ggtgaaccgc acgagcaacc gactgagaat gtcgctcttg
     7681 cgcagctcga acacgtcttg gccggccgcc gcgctcatgg cgtacgggcg ggccgattcc
     7741 agcagcgtgt gccgccgtgt gcggtggaag aactcctcgg ggcgtaccga ggaggcgggc
     7801 accaggatgc cctgcatgct ctgcgtctgg ccgagcgggg tggtggcgat gggcgcgggc
     7861 attagccgtt ccttcgggtc acgtagtcat tgagggtccg caaacccggg acggcgttcc
     7921 cgagacggtc ggcggcgatg ttgaacagca cgggagcgat caggctcacg ccgccgacgg
     7981 cgagccagaa cgcggggagc tgggcgcgct tcacgggggg aaggtcagcg tccgagccgg
     8041 acggcgggag cgccggcgcc acgggacttg tacgcctcgt agctgacgac gacggccagg
     8101 gcgatggccg cggtgtacgc gatccggcga gcgttcatga ggtggggcct ccgttgagcg
     8161 gtggtggtag tgctgcacca cctaacggag caaacaccgc cgtgcctgcg caagggctcg
     8221 ggcacggtta acccgggtga acttgtcagc gggacagggt catccggtag ccgagcagca
     8281 tgacccacga cttgaacgtc gccagatact cacgcctggg cgcgtcgagg atctgccacg
     8341 acgcctcgaa ggggaggctg taggggtgct cggcgagcga ggagtgcagc gtgatgaagg
     8401 cgtgttcgag gtcggtcgcg ccatagttcc acggctcggc cgcgatctcg tgcgccttcg
     8461 cgagctggcg ggccccgaag tcgcgatcat cggcggtgaa ggcggtgctg ggcatgatgg
     8521 tctcccggtc gaaggtgggc gggtagggca tggttcagat gggtaggggt gggcaccgat
     8581 agagccgctt cgtcgcggtc acccatagca ggaaccagta gtccccgcga cggaatgtct
     8641 cgtcacactc gatggcgaac ctcgccggtg gatagccgat gaccttcgcg acgcgcactc
     8701 ggtcgtccgg gtcgggcagg tcgaagacgg cgacgatgtc gctctgcgcg atgacgagct
     8761 tgtcgatgtc gatgggccgt ggcccgtcga acagggcgga cgtgcggtag tgccgggact
     8821 gcatgagcag tcggcgcaga tgtgggccgg tccggtttcc ggtggtgaac tcgccgacct
     8881 cgccggccca cacgagcacc tgcacggctg aggggtacat cgccatgccg atggcgcggt
     8941 ctaggtccac ggcatatgtg ctcgacccgg ggtcagcgac ccaatgcagg ttggccggct
     9001 tgccgtcgcg gtccgggccg ggcatcttct ggtcgatctc gcggatccgc ttcgcgtccg
     9061 ggccggggtc ggcctcgccg ttcacgtcga tgcacagctt cgcgtacggg tagtcccggt
     9121 acagctcgcg gttaaacgtc gtctttccgg agcccttccg gccgaacgcc gagacgatcc
     9181 agttacgttg cgggtcaagg cgggcgggcc aaccgctgcc ctccgccacg tcgtcggtca
     9241 cgccggcggc gccgtgtcgt acgccgatgg cacgttggtg ggtggcgtgg gcggggcgac
     9301 ttccggctgg aacccgtcga ccgcggctcc ggatcgacgc atcgagcgag cgaatcggat
     9361 cttgtgcatc tgcttcacga gccagaacac cagggcgatg gcgagcgaga ttccatcggc
     9421 cacgtcggga tttccggcgc cgaggtcccc ggtgcgccgg gacatgatac tcgcggcagg
     9481 gtcggcgatc ccgtcgacgt cctgcgggtc ggccagccac acgccggcga ccttgtcgta
     9541 atcgtcgcga gcgagtactt cgtggaccac aacgctggca ccactgaccg cggcggcgat
     9601 cccgtggcga agcgtctcgg catcaccggg cgaggatgcc cgggatgatc cacgccggct
     9661 cttgcccttc gggctggggg tcggctcggc gtcgggcgcg ggagcgccat cgtccactcg
     9721 cgtgtcgagg ttggggctct cgctcgggct cgtcgcgtcc gtcgcgtcca gctggtcgag
     9781 gatcgggtcc ggggctgggg ggctgctgaa cctcggtcgt ccgaccctcg cgcgggcggg
     9841 ctggtcctgg tcctggcttg gggtcgtcac ggtcgtactc cacctcgatc acgtcgtgcg
     9901 ggtctggcgg gggcggggat tgcttgccgc cggtgacccc cgaccccgcg ccgggtgagg
     9961 ggtcgggggc gcctgggatg cgccggccct tgacggtgag ggtgcagccc tcggtgctgc
    10021 atggtccctc ccaggtcacc cgccccgggt cgctcaccgc ttcggtgacg tgcccggctt
    10081 tgcaggtgcc tgcgaccttc actcgaccgc gatggtttgg cacgctcccc aacggatcac
    10141 acactcggcc ggtcatggaa gtgaccaggg acagatttcc gcttggcgag ccatcgagca
    10201 tgtccgacgt ggtacagccc gcaccggcag cggtaggtcc cgagcagccc gcggtcggga
    10261 tctttcgatg tactccacag gtgccagagc tggcgtttcg ccgcctccgc ggtcttgaac
    10321 ccccgcttgg acggacgtgg acagttcggc gcgcccatgg tcacgcggcc ctggttggga
    10381 tgtgtcgcat gtccttcgcc gcgccgcatt cgcaacggca cgaacggttt cggtggcccc
    10441 ggactcgggt gcaccggtgc tcactgccgc tgtcccagct cacccaggtc tcgcggcacg
    10501 ggtctcggtc cctggcctga cgggcatact cgccgcgaac cacagcggcg cccgcgatca
    10561 gggcggcgag gtctgtcagg accgtcacgg ggtccatcac tgtccccatt cccacttcgg
    10621 cgacctctcg gtcatagcgt ttcactcctc ttcctgagcg ttcggcggcg tagttgggcg
    10681 cgctgttgat cggtcagccc cccccatacg aaatggtgga tacgcagggc ctgggcgtag
    10741 gcgaggcagt cggcaacgac cgggcagccg ttgcagacac gtgcggcttc gcgctgatcg
    10801 cggtcggtga agtcgggcat ctggttgcag gcggcgcgat ccatccatga ccgatccggc
    10861 tcggggggct tcttgacgcc gggcattggg tccttgggcc tgtcgacccg agggaggcct
    10921 gtcgcggcgg accaccctcc gcggtagacg accagccgta cccggtttga tgtcaccccg
    10981 tagtcctcgg cgagctggcg cacggtcgcc ccgtcgcgga tctgcttggc gagcgctccc
    11041 gagccgggca gtcgtgccgg tgccgcgtgg tcgcgtccca cggtcgcgaa cgccgccgcg
    11101 ctcatgctcc ggcccccagg atgatgccga cgatccggct cttcgtgacg ccggctgcgg
    11161 cggcgatctc ccggtgggac agtcccgcgt cgacggcggc gaggatgaac tgatcgcgca
    11221 gccggacggc caggcgccag ccgtcctcgg catcgcgcgc tatggccgcg gcggcagcga
    11281 tctttgggac gaggtcgggc aagacttcgg cgggcatggg tcatcgcctc tctcgcgcgg
    11341 cggcgatacg cgcgcgcagg tcttcagggg gtggggtcag ggtcgggtcg ggtgttcggg
    11401 aggcgagctg gcgggccagg cgcgcgacgc gatcctcggc gcgctgttgt tcgcgcaccg
    11461 cccgagcctg ggtccgtgag tcagtcccgc agcggcggca accctgcatg gccgttcgtc
    11521 cgtgggggca tttcaagggg gccccaccga ccaggtcggt gggggagtcg ctaggcgctg
    11581 gaagcggcgc cggcaggctt gggggagggg gggtatttct ggtagaaaac tggatacgcg
    11641 cgcgcgcgcg gacggggggg acaaactgac ccccctgggg ggacagactg acccccctgg
    11701 ggggacaaac tgacccccct ggggggacag attcacccgg gtcaaccgtg gagaggttca
    11761 cccgggtttc cccctcggtc agtcgggtga c
//
LOCUS       DAORPW010000071        11484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1239_length_11484_cov_762.628314, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000071 DAORPW010000000
VERSION     DAORPW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11484
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1239_length_11484_cov_762.628314"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLB86_07330"
     CDS             <1..736
                     /locus_tag="PLB86_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRC64987.1"
                     /translation="FLLTLAVVDDLVAITIIALFFSSDVQPLMLAGALIPLAVWGWLL
                     RRGWTYPLLLAVPAVATWALVHEAGVHATVAGIVLGLTVPVARSNDADDSTEGHSLSE
                     QLEHGMRPYSAGLAVPLFAFFAAGVKITSDGLATAFRDPVTIGIIIGLVAGKSLGVFS
                     GTWLVARFTRAQLDEDLAWADIAGLSLLAGLGFTVSLLIGELAFGSGSARDDHVRVAV
                     LLASLVAAVLASIVLRRRDAHYRQLQ"
     gene            806..1216
                     /locus_tag="PLB86_07335"
     CDS             806..1216
                     /locus_tag="PLB86_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRC64988.1"
                     /translation="MATDAPPLISDAEPTLGQLVASASRDVSTILRSEIALAKTEITG
                     AAKFAGKGAGMFAGAAFFGLFGLGFLLTALAWGLVALGLATWLAFLIVAVLLFIGAAV
                     LALVGKKALAHADPTPKRAISEAQQTIATIKGQS"
     gene            1258..2169
                     /locus_tag="PLB86_07340"
     CDS             1258..2169
                     /locus_tag="PLB86_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC64989.1"
                     /translation="MSDAASVLLDGPWEHEYVAANGARFHVALAGDGPLVLLLHDFPQ
                     FWWAWRHQLVALADAGYRAAAVDLRGFGASDKPPRGYDTYTATADAAAVIRSLGEERA
                     VVVGAGLGGWTAWAMPNLQPAFTRGIGAVSMAHPAIMQRAARRDRAQRSALSFVGHLQ
                     WPFRPERSLIRDDTLVRRYLTSWAAPGGTFPSEEEISRYAAALALPFVAHSAAEYYRW
                     FGRNQVRRDGPLFQRRVGSPIQVPVLQVQGSADGCVLADATGGSQRYVSGPYTYQAIE
                     GAGHFLPEEAPTQVNRALIDWLATVPA"
     assembly_gap    2174..2273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2286..3494)
                     /locus_tag="PLB86_07345"
     CDS             complement(2286..3494)
                     /locus_tag="PLB86_07345"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HRC64990.1"
                     /translation="MTGGMLLDIVLVVLLGSYAVSGFGQGLVVSVLSLVGFLGGGALG
                     MAVLPGWFDGWAWAGEHPFESRALLIGGVFFLAALGQGIAVHVARRMRRHLHAGPIVA
                     ADAVLGGVASLIAVAVLVWFVAAGLRGSTASIVARTIGESRVLAVVDALVPPQTGALF
                     AGFRQVLDRGGFPRVFEGLTTEPIIAVDPPSSAVLGGAGVVAAQASMVKVTGVAAACR
                     RGQEGSGWVVAPGRVVTNAHVVAGIEDVTVRVRGTGPALHGSVVVFDPQRDLAVISVP
                     GLNAPALPLGADLARGAEAVVAGFPLDGPLRLDAARVRQVINATGQDIYGQPGVTREI
                     YSLLTRVEPGNSGGPLLSSAGQVVGVVFAKSLDDDQTGYALTLAEAAPVLTAGAAAQG
                     RVDTGACSLG"
     gene            complement(3491..4213)
                     /locus_tag="PLB86_07350"
     CDS             complement(3491..4213)
                     /locus_tag="PLB86_07350"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRC64991.1"
                     /translation="MSSDVSRVAHGTPLVPLVPLGAGMPRPRPAWVDGILEGARAEPE
                     WFTRYLPPDGDQRQSAVLILFGPGPQGEDSLLFIERAHTLRSHAAQISFPGGGVDPED
                     DNVVAAALRESFEEVGLDPTGVDIVGTLPELFLPVSAYTVHPVLAWWKDPSPVYVGHP
                     DEVAQVLSVPVSYLADPANRHTVSHPSGYRGPAWDLGDDLLLWGFTGGIVNKILELAG
                     FAREWDSDHLVPLPERFMRRSS"
     gene            complement(4210..4938)
                     /gene="nth"
                     /locus_tag="PLB86_07355"
     CDS             complement(4210..4938)
                     /gene="nth"
                     /locus_tag="PLB86_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRC64992.1"
                     /translation="MPADRAAPASETPVALTRRARRIYRALLERYPYAHCELDFTTPL
                     ELLVATILSAQTTDVRVNQVTPTLFARYRTAAEYAAADRADLEALLGPLGFFRAKTES
                     LLKLGAALVERFDGEVPGRLDDLVTLPGVGRKTANVVLGNAFGVPGITVDTHFGRLVR
                     RFGWTTEEDPVKVEHAVGALFPRKDWTMLSHVVIFHGRRTCHARKPACGACPVAQWCP
                     AYGTGETDPEKAARLLTYELAPTS"
     gene            5068..5745
                     /locus_tag="PLB86_07360"
     CDS             5068..5745
                     /locus_tag="PLB86_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRC64993.1"
                     /translation="MDHDVVRRAPLFAALDDEAGQALLAQMNSSRLERGDILFREGDP
                     GDTLYVIGEGKVKLGRTSADGRENLIAILGPGEMFGELSLFDPGPRTMTATAVAETQL
                     MGLGNDSLTELLTGRPEVAKALMAALAARLRRTNEHLADLVFTDVPGRVAKALLDLAN
                     RFGRPVEDGIMVAHDLTQEELAQLVGASRETVNKALADFATRGWLKLEARAVLLLDVE
                     RLKRRAR"
     gene            5800..6678
                     /locus_tag="PLB86_07365"
     CDS             5800..6678
                     /locus_tag="PLB86_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4261 domain-containing protein"
                     /protein_id="HRC64994.1"
                     /translation="MGLIDKIRSSLPIPGRSFRHPEPAAQLLAFVLLERTIGVDEIVA
                     LVATVPGASATPMAHSPSSAEATDPTETSGPVEITLGGTTGFVLEISAPVPDGEAEAS
                     AANNAFWPDAAAAVARHRAHLIVSVSDQRADGVGDADGADPGERHTQRYAAFAHLVAA
                     LLGHEATVGVYLGDQGVVYEPAFYIDAVAGAGDGLPWEVAWFTWAAWESEGVSCGSTR
                     GLRVFGHEELEVRGSTAAPSAVMDLLANIASYVVESGAVLMPGETLGYTDDQKLTITA
                     VEASFVEGHTLAIGLP"
     gene            complement(6675..7412)
                     /locus_tag="PLB86_07370"
     CDS             complement(6675..7412)
                     /locus_tag="PLB86_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC64995.1"
                     /translation="MTLDGTNTWVLREPGAQECIVVDPGPLDEAHLTRVLDVVRRDGG
                     RVGLTLLTHDHSDHADAAERFAHLSGAPVRAIRAIGTGHDDLADGDSVSVGGLDLVVV
                     TTPGHTSDSVSFLLPAECAVLTGDTILGRGTTVVAWPDGELAAYLASLERLEALCGSG
                     AVARILPGHGPHLPDAGAVLRYYLEHRAQRLEQVRGAVAAGARTPWEVVEAVYADVPR
                     AVWGAAEWSVRAQLAYLAERGEEGQLG"
     gene            complement(7502..8380)
                     /locus_tag="PLB86_07375"
     CDS             complement(7502..8380)
                     /locus_tag="PLB86_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRC64996.1"
                     /translation="MIRDFSTSNAPALHATALAWLSGEHGEPAPLRPAATVMIVRDAS
                     DDESGNSGGSPVEVFLLRRVASMAFAPSTIVFPGGGVDPRDSAELPWAGPGVLEWSVR
                     LGADEEATRKLVVAAVREVFEECGVLFASADEDGPLVEVSSPQWQQVRAGLVARELSL
                     SDVLREHGLCLRTDLLRAHAHWVTPVCEPRRFDTRFFVALMPAGRAIDGATSEADHAG
                     WFDPASVLADYAAGSAVVLPPTLVCLEDLAASPSAAEFFAQEPVIRRIMPELVVNDAG
                     EAVMRTDFAGTKAPRG"
     gene            complement(8395..8868)
                     /locus_tag="PLB86_07380"
     CDS             complement(8395..8868)
                     /locus_tag="PLB86_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRC64997.1"
                     /translation="MSAVEERLAAMGYSVPDVVPPVAAYVPALRQGDWVFVSGQLPMR
                     DGAMPATGKVGDGPGQVSPEAAKQLAAFCALNGIAAVKSVIGDLDKVVRIVKVVGFVA
                     ADPSFTGVPGVVNGASELLGAVFGDAGVHARSAVGVSVLPLDAPVEVELIVAVRD"
     gene            complement(8865..9032)
                     /locus_tag="PLB86_07385"
     CDS             complement(8865..9032)
                     /locus_tag="PLB86_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HRC64998.1"
                     /translation="MTSWEYATVPLIVHATKAILDNWGRDGWELVQVVPGPDGNGLVA
                     YLKRPLAGGQA"
     gene            9123..>9784
                     /locus_tag="PLB86_07390"
     CDS             9123..>9784
                     /locus_tag="PLB86_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HRC64999.1"
                     /translation="MPPAPDPLRADGFAGVRLHVVTGKGGTGKTTVAAALAVALANEG
                     LRVLLAEVEGRQGISQTFDVPPLPTTEVRVAGGAQGGEVVGLAVDAKTALLEYLQLFY
                     KLGRAGGVLERFGAIDFATTIAPGVRDLLLIGRVYEASRRRHDAKHHRGSPPEELAYD
                     AIVLDAPPTGRIPRFLSVGAEVAGLARMGPIHSQAGSITELLHSDATRVHVVTLLEEM
                     PV"
     assembly_gap    9785..9884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9935..11086
                     /locus_tag="PLB86_07395"
     CDS             9935..11086
                     /locus_tag="PLB86_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HRC65000.1"
                     /translation="MARTPVLDIDAMLADRHTRIVVCCGSGGVGKTTTAAALALRAAE
                     AGREVVVLTIDPARRLAQSLGLTELDNVPRPVSGLADDAVPGGRLDAMMLDMKRTFDE
                     VVLAHSTREAAEQILANPFYQAVSSSFAGTQEYMAMEKLGQLRARADREGTWDLIVVD
                     TPPSRSALDFLDAPKRLGSFLDGRFIRLIAAPAKMGGRAGLRLVNVGIGLVSKTLTTV
                     LGGEMLRDVQTFVNALDTMFGGFRERADLTYALLKEGGTEFIVVAAPERDALREASYF
                     VARLEQEGMPLAGVVVNRMQPSAAPGLTAARARAAAEQLAEADRPAAAAALSLHAEVS
                     ELAARHRNLVRRFTAGHPSIPVVEVASLAQDVHDADGLREVGRRLAGLP"
     gene            complement(11139..11447)
                     /locus_tag="PLB86_07400"
     CDS             complement(11139..11447)
                     /locus_tag="PLB86_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRC65001.1"
                     /translation="MSTPILRGALRQDWAGLGACVSGDPDDLFVKGAAQQQAKEVCQR
                     CPVIAECLAEALDNRTEFGVWGGMTERERRALLRRNPTVTSWWAMFAQAGAGRADVAS
                     "
BASE COUNT         1689 a         4125 c         3898 g         1572 t
ORIGIN      
        1 gttcctgctc accctggccg tcgtcgacga cctcgtcgcg atcaccatca tcgcgctgtt
       61 cttctcgtcc gacgtgcagc cattgatgct cgccggtgcc ctgatcccgc tggcggtctg
      121 ggggtggctg cttcggcgtg ggtggacgta tccgctgctg ctggccgttc cggcggtcgc
      181 cacgtgggcc ctggtccacg aggccggcgt ccacgccacg gtggccggca tcgtgctcgg
      241 cctgaccgtc cccgtcgccc gttcgaacga cgccgacgac tcgacggagg gccactcgct
      301 ctccgagcaa ctcgagcacg ggatgcggcc ctactccgcc ggactcgccg tgccgttgtt
      361 cgcgttcttc gcggcggggg tgaagatcac gtccgacggg ctcgccaccg ccttccgcga
      421 cccagtcacc atcggcatca tcatcggcct ggtcgcgggc aagtcgctgg gcgtcttcag
      481 cggcacctgg ttggtggccc gattcacgcg cgcccaactc gacgaggacc tcgcctgggc
      541 cgacatcgcc ggcctgtccc tgctggccgg actggggttc accgtgtcgc tgctcatcgg
      601 ggagttggcg ttcggctcgg gcagcgcccg cgacgaccac gtgcgcgtcg ctgtgctcct
      661 ggcctcactc gtcgccgccg tgctggcctc gatcgtgctg cgtcggcgcg acgcccacta
      721 ccggcagctg cagtagcgcg acataccgca gtgggacatg cggcgatagg ctcgcctcca
      781 ggagcaccac cccaggagga cacccatggc tactgacgcg ccaccgctca tcagcgatgc
      841 cgagcccacg ctcggccagc tcgtcgcgtc cgcctcccgg gacgtctcga cgatcctgcg
      901 ctccgagatc gcgctcgcca agaccgagat caccggcgcc gccaagtttg cgggcaaggg
      961 cgccgggatg ttcgcggggg cggcgttctt cggcctgttc ggcctgggct tcctgctcac
     1021 ggcgctcgcg tggggtctgg tcgcgctggg gctggccacg tggttggcct tcctcatcgt
     1081 cgcggtcctg ctgttcatcg gggcggcggt cctggccctc gtgggtaaga aggccctggc
     1141 ccacgccgac ccgaccccca agcgagccat ctccgaagcc cagcagacga tcgccacgat
     1201 caagggccag tcctgacctc tcccgcggtg cccctgcagc accgctcaag gaatgacatg
     1261 tccgatgccg cctcggtcct cctggacggc ccgtgggagc acgagtacgt cgctgccaac
     1321 ggtgcccgtt tccacgtggc cctcgctggc gacggtccgc tcgtgctgct cctgcacgac
     1381 tttccgcagt tctggtgggc ctggcgccac caactcgtcg ccctagccga cgcgggatat
     1441 cgagcagcag ccgtcgacct gcgcggtttc ggcgcctcag acaagccgcc gcgaggctat
     1501 gacacctaca cggcgaccgc cgatgccgct gcggtgatcc gcagcctcgg cgaagaacgc
     1561 gccgtcgtcg tcggcgccgg gctcggcggc tggaccgcct gggccatgcc caacctgcaa
     1621 cccgcgttca cccgcggcat cggcgcggtg tcgatggctc atccggcgat catgcaacgg
     1681 gcggcccgcc gcgaccgcgc ccaacgctcg gccctgagct tcgtcggtca cctgcagtgg
     1741 cccttccgcc ccgagcgttc cctgatccgc gacgacaccc tggtccgccg ctacctcacc
     1801 tcttgggccg ccccgggcgg caccttcccg tcggaggagg agatcagcag gtatgccgcg
     1861 gcgctggcgc tcccgttcgt cgcccactcc gccgccgagt actaccgctg gttcggacgc
     1921 aaccaggtgc ggcgcgacgg cccgctgttc caacgccggg tcggtagccc catccaggtg
     1981 ccggtgctgc aggtgcaggg ctcggccgat ggctgtgtcc tggccgacgc cacgggtggc
     2041 tcgcagcgct atgtcagcgg cccctacacc tatcaagcca tcgagggcgc cggccacttc
     2101 ctccccgagg aagccccgac gcaggtcaac cgggccctca tcgactggct cgccaccgtc
     2161 ccggcctgag ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggccgaa
     2281 actggtcacc ccagggagca ggctcccgtg tcgacccggc cctgcgccgc cgcacctgcg
     2341 gtcaacacgg gcgcggcctc cgcgagggtg agcgcatacc cggtctggtc gtcatcgagc
     2401 gacttggcga agacgacgcc gacgacctgg ccggcgctgg acagcagtgg gccgccgctg
     2461 ttgcccggct cgacccgagt cagcagcgag tagatctcgc gggtgacccc cggctgtccg
     2521 tagatgtcct gaccggtcgc attgatgacc tgccggaccc gagcggcgtc cagtcgcaac
     2581 ggcccgtcga gcgggaatcc ggcgacgacc gcttcggccc cacgcgccag atcggcgccg
     2641 agcggcagcg ccggggcgtt caggccgggc accgagatga cggccagatc gcgctggggg
     2701 tcgaagacga cgaccgagcc atgcaacgcc ggcccggttc ccctgacccg gacggtgacg
     2761 tcctcgatgc ccgccacgac atgcgcgttg gtgacgaccc gacccggggc aacgacccag
     2821 ccgctgccct cctgcccgcg ccgacaggcc gcggcgactc cggtcacctt gaccatcgac
     2881 gcctgggccg cgacgactcc cgcgccgccg aggacggccg atgacggggg gtcgacggcg
     2941 atgatcggct cggtcgtcag accttcgaag acccgtggga agccgcctcg gtcgagcact
     3001 tggcggaacc ccgcgaagag cgcacccgtc tggggcggga cgagcgcatc gaccaccgcg
     3061 agcacccggg actcgccgat ggtccgagcg acgatcgacg ccgtgcttcc gcgcaggccc
     3121 gccgcgacga accacacgag gacagcgacg gcgatcaggc tcgcgacgcc gcccagcacc
     3181 gcgtcggccg cgacgatcgg tccggcatgc agatgtcggc gcatccgccg cgcgacgtga
     3241 acggcgatgc cctgacccag cgccgcgagg aagaagaccc cgccgatgag cagggcccgc
     3301 gattcgaagg gatgttcacc ggcccaggcc cacccgtcga accacccggg caacaccgcc
     3361 atgcccaacg ccccgccccc gaggaacccc acgagcgaca acaccgacac gacgagcccc
     3421 tggccgaagc cggacacggc atacgacccg agcaggacga ccagcacgat gtcgaggagc
     3481 atcccgccgg tcacgaggac ctccgcatga accgctccgg caacggcacc aggtgatcgc
     3541 tgtcccactc gcgggcgaag cccgccaact cgagaatctt gttgacgatg cccccggtga
     3601 acccccacag cagcaggtcg tcaccgaggt cccacgccgg tccgcgatag cccgaggggt
     3661 gcgagaccgt atgccggttg gccgggtccg cgaggtaact caccgggacg ctgagcacct
     3721 gagccacctc gtcggggtgt ccgacgtaaa cgggcgacgg gtctttccac caggccagca
     3781 ccgggtgcac ggtgtaggcg gagaccggta agaacaactc gggcaacgtg ccaacgatgt
     3841 ccacacccgt cgggtccagc ccgacctcct cgaacgactc ccgcagggca gcagccacca
     3901 cgttgtcgtc ctcggggtcc actccgccgc cggggaagga gatctgcgcg gcgtgggatc
     3961 gcaaggtgtg ggcccgctcg atgaacagca gcgagtcctc gccctggggg ccaggcccga
     4021 agaggatgag gacggccgac tgacgctggt caccgtccgg tggcagatac cgggtgaacc
     4081 actcgggctc ggcgcgggcc ccctcgagga tcccgtcgac ccacgcgggc cgcggacgcg
     4141 gcataccggc gcccagggga accagcggga cgaggggggt gccgtgggcg acccgcgaga
     4201 cgtcggagct cacgatgtcg gcgccaactc gtaggtgagc agccgggcgg ccttctcggg
     4261 gtcggtctcg ccggtgccgt aggcaggaca ccactgcgcg accgggcagg cgccgcacgc
     4321 gggcttgcgg gcgtggcagg tgcgccggcc gtggaagatg acgacgtggc tgagcatcgt
     4381 ccagtccttg cgggggaaca gcgccccgac ggcgtgctcg accttgaccg ggtcctcctc
     4441 cgtcgtccac ccgaagcggc gcacgagccg cccaaagtga gtatcgacgg tgatcccggg
     4501 gacgccaaaa gcgttgccta gcaccacgtt tgcggtcttg cgccccacgc ccggcaaggt
     4561 caccaggtca tcgagacggc cgggcacctc cccgtcgaag cgttcgacga gcgccgcacc
     4621 cagcttgagc agcgactcgg tcttggcccg gaaaaagccc agcggcccga ggagggcttc
     4681 gaggtcggcc cggtcggcgg cggcatactc ggcggccgtc cggtagcgcg cgaacagggt
     4741 cggggtcacc tggttgaccc gcacgtcggt cgtctgcgcc gagaggatcg tcgcgacgag
     4801 caactccagc ggcgtcgtga agtcgagctc gcagtgggcg tagggatacc gctccagcag
     4861 cgcgcgatag atccgccgag cccgtcgggt cagtgccacg ggcgtctcgg aagcgggggc
     4921 agcacggtct gcgggcacga gggacagggt atgccgcctg cctgggcaga gcgatcatcc
     4981 cggcgcgctg aacagcagag tccggagggc ggcatacggg accaacctcc cggacagttg
     5041 acgacccggc atggcacact gtgcgacgtg gatcacgatg tggtgcgccg ggcgccgttg
     5101 ttcgcggccc tcgacgacga ggccggtcag gcgctgctcg cgcagatgaa ctcttcgcgg
     5161 ctcgaacgag gcgacatcct ctttcgggaa ggcgacccag gcgacaccct ctatgtcatc
     5221 ggcgagggca aggtcaaact cggccgcacc agcgccgacg gccgcgagaa cctcatcgcg
     5281 atcctcgggc cgggcgagat gttcggcgag ctgtccctgt tcgaccccgg gccccggacg
     5341 atgacggcca ccgccgtcgc cgagacccag ctcatggggc tcggcaacga ctcactgacg
     5401 gagctgctca ccggccgtcc cgaagtcgcc aaagccctca tggccgcact cgccgcgcgg
     5461 ttgcgtcgca ccaacgagca cctcgcggac ctcgtcttca ccgacgtgcc cggccgggtc
     5521 gccaaggccc tgcttgacct ggccaaccgg ttcggtcgcc cggtcgaaga cggcatcatg
     5581 gtcgcccacg acctcaccca agaggagttg gcacagctcg tcggggcatc ccgcgagacc
     5641 gtcaacaagg cgctggccga cttcgccacg cgtggttggc tcaagttgga agcccgcgcg
     5701 gtgctgctgc tggatgtcga gcgactcaag cgacgcgcgc gctgacccga acggccgggc
     5761 gggcagctgc ccggccaccc gacgcggagg gggcctgtca tgggcttgat cgacaagatc
     5821 cgttcttcgt tgccgattcc tgggcgcagt ttccgccacc ccgagcccgc ggcccagttg
     5881 ctcgcgttcg tgctcctgga gcggacgatc ggcgtcgatg agatcgtggc gctcgtcgcc
     5941 accgtcccgg gtgcatccgc cacgccgatg gcgcactcgc cgtccagcgc cgaggccacc
     6001 gaccccaccg agacctccgg gccggtcgag atcaccctgg gcggcacgac cggcttcgtc
     6061 ctggagatca gcgctcccgt ccccgacggc gaggccgagg ccagtgccgc caacaacgcc
     6121 ttctggcccg atgccgcggc tgcagtggcc cgccaccggg cacacctcat cgtcagcgtg
     6181 tctgatcagc gagccgacgg cgtcggggac gcagacggcg cggacccggg cgaacggcat
     6241 acccagcggt atgccgcctt cgcgcacctc gtcgccgcgc tgctcgggca cgaggccacc
     6301 gtcggggtct acctgggcga ccagggcgtg gtctacgagc cggccttcta catcgacgcg
     6361 gtcgcgggcg ccggggacgg cctgccctgg gaggtggcct ggttcacctg ggccgcctgg
     6421 gagagcgagg gcgtgtcctg cggctccacg cgggggctgc gagtgttcgg gcacgaggag
     6481 ctcgaggtcc gtgggtccac cgcggcgccg agcgccgtca tggacctgct ggccaacatc
     6541 gccagctacg tcgtcgaatc gggtgcggtg ctcatgcccg gcgagacctt gggttacacc
     6601 gacgaccaga agctgacgat caccgcggtc gaggcctcgt tcgtcgaggg ccacaccctg
     6661 gcgatcggcc tgccctagcc cagctggcct tcctcgccgc gctcggcgag ataggccagt
     6721 tgagcccgca cgctccattc cgcagcgccc cagacggccc ggggcacatc ggcatacacc
     6781 gcctcgacga cctcccacgg agtgcgcgca ccggcggcga ccgccccacg cacctgctcc
     6841 aggcgctgcg cgcggtgctc gaggtagtag cgcagcaccg ctcccgcatc gggcaggtgc
     6901 gggccgtggc cgggcaggat ccgcgccacc gcgcccgacc cacagagggc ttccagccgc
     6961 tccagcgagg cgaggtatgc cgcgagctca ccatcgggcc aggccacgac ggtcgtgccg
     7021 cgcccgagga tcgtgtcgcc cgtgaggacg gcgcactcgg ccggcaagag gaagctcacc
     7081 gaatcactcg tatgccccgg cgtggtgacg acgaccaggt ccagcccgcc gacgctcacc
     7141 gagtcgccgt cggccaggtc gtcgtggccg gtgccgatgg cccggatggc tcgcaccggc
     7201 gcgccggaca ggtgggcgaa gcgctcggcg gcatcggcgt ggtcggaatg gtcgtgggtg
     7261 agcagggtca gcccgactcg gcccccgtcg cgccggacga cgtcgagaac ccgcgtcaga
     7321 tgggcctcgt ccagcgggcc cgggtcgacg acgatgcact cttgcgctcc cggctcgcgc
     7381 agcacccatg tgttggtgcc gtcgagggtc atcaccccgg ggttgggagc taagacgcag
     7441 taggcccgct ccgtcacctg cccggatgcc caggtgggtg gcactggcgg gagcgtgaga
     7501 gtcacccgcg cggagccttg gtgcccgcga aatcggtgcg catcacggcc tcgccggcgt
     7561 cgttgacgac caactcgggc atgatccggc ggatcaccgg ctcctgcgcg aagaactcgg
     7621 cagccgacgg ggaggctgcg aggtcctcca ggcagaccag agtcggcggc agcaccaccg
     7681 cggaaccggc ggcatagtcg gccaggacag atgccgggtc gaaccagccg gcgtggtcgg
     7741 cctcgctcgt cgcaccgtcg atggcccggc ccgccggcat gagcgccacg aagaaccgcg
     7801 tgtcgaagcg tcgcggctcg cagactgggg tcacccagtg ggcgtgggcg cgcaggagat
     7861 cggtgcgcag acacaacccg tgctcacgca gcacgtcact caacgacaac tcgcgggcga
     7921 ccaggccggc gcggacctgc tgccactgcg ggctggacac ctcgaccaga ggcccgtcct
     7981 cgtcggcgct ggcgaacagc accccgcact cctcgaagac ttcccgcacc gcggcaacca
     8041 cgagtttgcg ggtggcctcc tcgtcggcgc cgaggcggac cgaccattcc aggacgccgg
     8101 ggccggccca cggcaactcc gcgctgtcac gggggtcgac tcccccgccg gggaagacga
     8161 tcgtgctcgg ggcgaaggcc atcgacgcga cccggcgcag caggaacacc tcgacggggc
     8221 tgccgccaga gttgccagac tcgtcatccg aggcgtcgcg gacgatcatc acggtggcgg
     8281 ccgggcgcag cggcgcaggc tcgccgtgtt caccggacag ccacgccaag gctgtcgcgt
     8341 gcaacgcggg agcgttgctg gtggagaagt cgcggatcat gcccggcgcc cgcgtcagtc
     8401 gcggacggcg acgatgagct cgacctcgac gggggcatcg agcggcagca cgctcacacc
     8461 caccgccgat cgggcatgga cgccggcgtc gccgaagaca gcgccgagca actcgctcgc
     8521 cccgttgacg actccaggca ctccggtgaa cgacgggtcg gcggcgacga agccgacgac
     8581 cttgacgatc cgcacgacct tgtcgaggtc accgatgacc gatttcaccg cggcgatgcc
     8641 gttgagcgcg cagaaggccg cgagttgctt ggccgcttcc gggctcacct gtccgggacc
     8701 gtcaccgacc ttcccggtcg cgggcatggc gccatcccgc atcggcagtt gccccgacac
     8761 gaacacccag tcgccttggc gcagcgcggg gacgtaggcc gcgaccggcg gcacgacgtc
     8821 agggaccgaa tagcccatcg ccgcaaggcg ctcctcgacg gcgctcatgc ctggccacca
     8881 gccagcggcc gcttgaggta ggccaccagg ccattgccgt ccggcccggg caccacctgg
     8941 acgagctccc agccgtcgcg gccccagttg tccaggatcg ccttcgtcgc gtggacgatg
     9001 agcggcaccg ttgcgtactc ccacgaagtc atgccggtca ctctaacggc gcgcaccgcc
     9061 cacaggcggc ctgcgggcag gacggcagcc gggcagcggc gcgacggcat acatttggcc
     9121 gtatgccgcc cgcccccgac ccgttgcgcg ccgacggttt tgccggtgtg cggctgcacg
     9181 tggtcaccgg caagggcggc accggcaaga cgacggtcgc cgcggccctg gccgtcgcgc
     9241 tggccaacga gggcctgcgg gtcctgctcg ccgaggtcga gggccgccaa ggcatcagcc
     9301 agaccttcga cgtcccgccg ttgccgacga ccgaggtccg cgtcgcgggc ggcgcgcagg
     9361 gcggcgaggt cgtcgggctg gctgtcgacg ccaagacggc gctgctggag tacctgcagt
     9421 tgttctacaa actcggtcgc gccggtgggg tgctggagcg tttcggggcc atcgacttcg
     9481 cgacgacgat cgcccccggg gtgcgcgacc tgctgctcat cggccgggtc tacgaagcca
     9541 gccgccgccg tcacgacgcg aagcaccacc gcggcagccc gcccgaggag ctcgcctacg
     9601 acgccatcgt cctggacgcg cccccgaccg ggcggatccc ccggttcctc tccgtcgggg
     9661 ccgaggtcgc cgggttggcg cggatgggcc cgatccactc gcaggccggc tcgatcaccg
     9721 agttgctgca ctccgacgcg acccgcgtgc acgtggtcac cctcctggag gagatgcccg
     9781 tgcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcggtg accgagatcg
     9901 ccgccctgct cgccgagcaa gggatggtgc cctgatggcc cgcacccccg tcctggacat
     9961 cgacgcgatg ctggccgacc ggcatacccg gatcgtcgtg tgttgtggct cgggcggtgt
    10021 cggcaagacg acgaccgcgg cagcgctcgc cctccgcgcc gccgaggcgg gccgcgaggt
    10081 cgtcgtgctc accatcgacc cggcgcggcg gctggcccag tcgctcgggc tcaccgagct
    10141 cgacaacgtg ccgaggccgg tcagcgggct ggccgacgat gcggtgccgg gtgggcgcct
    10201 cgacgcgatg atgctcgaca tgaagcggac cttcgacgag gtcgtgctcg cccattcgac
    10261 ccgcgaggcc gcggagcaga tcctcgccaa ccccttctat caggcggtgt cctcgtcgtt
    10321 cgcgggcacc caggaataca tggcgatgga gaagctcggg cagctgcggg cccgcgccga
    10381 ccgtgagggc acgtgggacc tcatcgtcgt cgacacgccc ccgtcccggt cggcgctgga
    10441 cttcctcgac gcccccaagc ggctcgggtc gttcctcgac gggcgcttca tccggctcat
    10501 cgccgcgccc gcgaagatgg gcgggcgggc cgggttgcgc ctggtcaacg tcggcatcgg
    10561 gctggtgtcc aagacgctga cgacggtgct cggcggggag atgctgcgcg acgtgcagac
    10621 cttcgtcaac gcgctcgaca cgatgttcgg cgggttccgc gagcgcgccg acctcaccta
    10681 cgcgttgctc aaggagggcg ggaccgagtt catcgtcgtc gccgccccgg agcgggacgc
    10741 gctgcgcgag gccagttact tcgtggcccg gctggagcag gagggtatgc cgctcgccgg
    10801 agtcgtcgtc aaccggatgc agccctctgc cgcaccgggg ctcaccgcgg ctcgggctcg
    10861 ggccgctgcc gagcagttgg ccgaggccga ccggccggcc gcagcggctg ccttgagcct
    10921 gcatgccgag gtctcggagt tggcggcgcg gcatcgcaac ctggtgcgcc ggttcaccgc
    10981 cggccacccg tcgatcccgg tggtcgaagt ggcatcgttg gcgcaagacg tccacgatgc
    11041 cgacgggcta cgcgaggtcg gccgccgatt ggccggccta ccgtgagtgc tcgactgccg
    11101 tgagtgcgcg aacggcaccc tgacggcgaa cctggccgtc agctggcgac gtcggcgcgg
    11161 ccggcaccgg cctgggcgaa catcgcccac caggaggtca ccgtggggtt gcgtcgcagc
    11221 agcgcgcgtc gctctcgctc ggtcatccca ccccacacac cgaactcggt gcggttgtcg
    11281 agtgcctcag cgaggcattc ggcgatgacg gggcagcgct ggcagacctc tttggcctgc
    11341 tgttgcgctg cacctttgac gaaaagatcg tccgggtccc ctgacacgca cgccccgagg
    11401 cccgcccagt cctggcgcaa agcgccacga aggatgggag tggacattcc tcctaccttt
    11461 cccgcagccc gttgaggaca aacc
//
LOCUS       DAORPW010000072        11426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1249_length_11426_cov_671.082842, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000072 DAORPW010000000
VERSION     DAORPW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1249_length_11426_cov_671.082842"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /locus_tag="PLB86_07405"
     CDS             complement(<1..1664)
                     /locus_tag="PLB86_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRC65002.1"
                     /translation="MQLNLTGLSMPRSSHAVTTLLNAVLAVGSGLDLSEVLTRIVRSA
                     CDLVGARYGALGVLRPDGEGLMEFVTSGLTPEEAGRVASQPFGHGVLGTLIRDPRPLR
                     LSAVGEHPDAVGFPPGHPVMTTFLGAPIHVRGEVFGNIYLADKVGGLEFTGEDEELIV
                     ALAAAAGMAIDNARMFHMARSGRQWSDALREMTQALLYGRDQRVALSMLVQRAQLAAE
                     AELAAVALAGEDGQLIIEASTGTDPRSRLIVGTPLAEPHWVTILDSGEPLLLLSREGE
                     AVADSLAAQLREASGLPPHGQTAIVPMSVGTGSLGLLVVAWGEGESHTAYDAAEPLKQ
                     YADLAAVTLTAASAQRDRARMVVLEDRERIARDMHDVVIQRLFATGLGLQSASRVAAH
                     PYVQKRLSEAVDELDLAIKDIRRMIFALHQPESQPLSEELHDLVVSFADPLGFTPRLT
                     VVGPLDDPETVDEWLRLDLLAVIREALSNVARHAQAGEVDVAVVVQADLVQVTVEDDG
                     VGMIETGRRSGLANLSERAAGRRGSLVVLSPMAVLGGTRVIWEVPA"
     gene            complement(1688..2113)
                     /locus_tag="PLB86_07410"
     CDS             complement(1688..2113)
                     /locus_tag="PLB86_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC65003.1"
                     /translation="MSTTRAQQGVPYTPGRSGDVVAGLLNDGRAESVAKAAVTEAVRR
                     GARLRFVQIVEEGLSPDERAEADQATFRAAVHALRGLRGIPCRFEVVTGDPTPTLLEL
                     TRGAAALIVGTDEGTARTIARRCVAHAECPVVTVPISAA"
     gene            complement(2231..2881)
                     /locus_tag="PLB86_07415"
     CDS             complement(2231..2881)
                     /locus_tag="PLB86_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC65004.1"
                     /translation="MTITVFLLDDHEIVRRGLRELLELSGDIEVVGESASALEAERRI
                     PALRPKVCVLDARLPDGSGIDVCRAVRAVDPTLRALILTSYDDDQALKAAVLAGASGY
                     VLKDIKGNDLVDAIRRVAAGEHLIRPEVMARVKSSLGESSVDPRLRSLTPQERRILEH
                     IAEGLTNRQIAEDMFLAEKTVKNYVTSILDKLGMDSRTQAAVYAATHPTRSRPRRP"
     gene            complement(2920..5946)
                     /locus_tag="PLB86_07420"
     CDS             complement(2920..5946)
                     /locus_tag="PLB86_07420"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HRC65005.1"
                     /translation="MARLGFAEPERSAGLLRDAVLLRLFAPGGDPGAGATLDTDALIE
                     STRAVRTSVAEGLATAADPDLAALGLVRLVEAVLGMPPAHPSDPRALAEVLSTDSVRR
                     RRLLLTLGGSRALVDHLAAHPEHWVAATDATPPTGPGLRAALTTAVTPQARGAATAYD
                     ALRVAYRRELVGIAALDLSDPDPVSHLPAAAEALAQLAEAALEAALDIAREEFGPGHE
                     DCRLAIIGMGKTGGGELNYVSDVDVIFVVEPADGVDEERAIKVGTALATATMKACAAQ
                     TGEGTLWPVDAALRPEGKAGPLVRTVASHKAYYERWAKTWEFQALLKAWVSAGDREVG
                     IAYKDALRSMIWGAAGREHFVEDVQAMRRRVEQHVPAAEAARQLKLGPGGLRDVEFSV
                     QLLQLVHGRHDESLRSATTLQALHALASGGYVGREDAATLDAAYRLLRTLEHRIQLYR
                     LRRTHLMPTAPDELRVLGRALGLTSKPAEAVVAMWQSQAREVRRIHERLFYRPLLAAA
                     ARLSSDDTSLSPQQARERLAALGFRDPAGAMRHLETLTAGVSRRAAIQRTLLPVMLSW
                     FADEADPDAGLLAFRRVSDDLGSIHWYLKMLRDEGSAAERLAHVLGRSRYAADLLIRA
                     PESVSILGDPDGLRPRSQESLAITLRSAAARKDNPDKAMAAVGALRRHELLRIVFGDL
                     TGQFAADQVGAALADLAGATVEAALEVATRVVGGGEDGLLVDVLVVGMGSLGGRELGY
                     ASDADVMVIHQPRPGVSDEAAHRQGLAIVQELVRLVAATGSDALVLDTDLRPEGKNGP
                     LTRSLASYAVYYDRWALTWEFQALLRARPIAGDRGLWEAFAALIDPLRYPAAGLDATQ
                     VREIRTMKARVESERLPRGADPRTHLKLGRGGLTDVEWTVQLLQLRHAHEVPSLQTPG
                     TMAGLRAATAAGLVGEEDSATLAAAYENASRLRNANLLWRGRPGDAVPTDIRDADGVG
                     RIVGRDPGTGAGLMEDYLRASRRARAVTESLFYESQ"
     gene            complement(6004..7341)
                     /locus_tag="PLB86_07425"
     CDS             complement(6004..7341)
                     /locus_tag="PLB86_07425"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRC65006.1"
                     /translation="MDRQQEFVLRTIEERDIRFVRLWFTDVVGTLKSVAVAPAELEGA
                     FAEGIGFDGSAIEGFARVYEADMLVKPDPSTFQVLPWRGENPGTARMFCDLLTPDGQP
                     SFADPRHVLRRALSRAANLGFTFYTHPEIEFFLLHPPKGKRITPVPVDDGGYFDHVPH
                     GTAHDFRRAAITLLENMGISVEFSHHEGAPGQNEIDLRYADALSTADNIMTFRTVVKE
                     VALEQGVRASFMPKPFREHPGSGMHTHLSLFEGDTNAFHEAGAPYQLSKTGRQFIAGL
                     LRHGAEITAVTNQWVNSYKRLWGGGEAPAYLTWGHNNRSALVRVPMYKPDKGNSTRVE
                     VRSLDSACNPYLALAVLLAAGLKGIEEGYELPPEAEDDVWSLTDSERRAMGIEPLPSS
                     LGRALELMERSELVAETLGEHVFDFFLRNKRAEWREYRQQITQFELDRYLTRL"
     gene            complement(7428..7643)
                     /locus_tag="PLB86_07430"
     CDS             complement(7428..7643)
                     /locus_tag="PLB86_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminopeptidase"
                     /protein_id="HRC65007.1"
                     /translation="MQYWYNVAKGVVENDDDRGPGEDVLGPYDTADEAARALESAHER
                     TEKWDAEDKAWNESGATTTWDDEELED"
     gene            7748..8611
                     /gene="map"
                     /locus_tag="PLB86_07435"
     CDS             7748..8611
                     /gene="map"
                     /locus_tag="PLB86_07435"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRC65008.1"
                     /translation="MPVSFASVAPHPVGPRRSVPVGIPRPEYVDKPAPARYQGSEVHD
                     AEMIERIRVAGRVAAQAMAAAAAAIAPGVTTDELDRIGHEFLLDHGAYPSTLGYRGFP
                     KSLCTSVNEVVCHGIPDSRRLQDGDIVNIDITAYLNGVHGDTDATYPVGEVDEESRLL
                     IERTHEAMMRGIKAVAPGRPINVIGRVISSYARRFGYGVVRDFTGHGIAEAFHSGLII
                     PHYDAAPSYATVMQPGMTFTIEPMLNLGTHEWDMWDDGWTVVTRDRRRSAQFEHTLVV
                     TETGSEILTLP"
     gene            8646..9398
                     /locus_tag="PLB86_07440"
     CDS             8646..9398
                     /locus_tag="PLB86_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRC65009.1"
                     /translation="MTSRVALGIDIGGSGIKGAPVDLDRGVLLADRVKIETPAESTPQ
                     AVAEIVRQIATHFIDTLPADAPIGVTIPGVVQHGVVRTAANIDKAWLDCPGEALFSAT
                     LGRRCLLVNDADAAGVAELRHGAAKDVEGLVIVTTLGTGIGIALLHNGVLIPNAELGH
                     LELDGHDAETRAAASAREREGLSWNKWAGRLQRYYTHLENLLWPDLFVVGGGVSRKSE
                     KFLPLLSLRTPIVPAELQNAAGIIGAAALAAD"
     gene            complement(9418..10071)
                     /locus_tag="PLB86_07445"
     CDS             complement(9418..10071)
                     /locus_tag="PLB86_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC65010.1"
                     /translation="MRITHLGHSCLFVELADRRILIDPGGFSSGFEDLTDLDAILVTH
                     QHADHLDQKRLPALVRANPGAAVHADPMSAALLVEAGLAVGVLAQGADLHLGDVRVRP
                     VGALHAFNHQWIPTVANVGVRLDAAGEPSLFHPGDAYDGEPGDIDVLAVPINAPWCAV
                     RDSIDFVRRLAPRSMVPIHDGLVAPPGRALYLTHIATNGGDDLTLHDLAGRGAVSIP"
     gene            10157..>11426
                     /gene="pgi"
                     /locus_tag="PLB86_07450"
     CDS             10157..>11426
                     /gene="pgi"
                     /locus_tag="PLB86_07450"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRC65011.1"
                     /translation="MTAPVDPTTTAAWSRLTSAATTLEPDLRAWFATDPTRAQRFSFE
                     CADLHVDLSKNLLTDDILAALVELGREVGLEERRGAMLAGERINVTEGRSVLHTALRR
                     PADAAPGLVLDGQDVDHDVHEVLAKVYAFADRVRSGDWRGVTGKRIETIVNVGIGGSD
                     LGPVMIYEALAPYVQAGLTCRFISNIDPTDAAQKTAGLDPETTLVIVASKTFTTLETI
                     TNARLVRTWLLDSLAASGAIDGSDASQADAVAKHFVAVSTALDKVAAFGIDPANAFGF
                     WDWVGGRYSVDSAIGTAVAVAIGPERFAELLAGFHAVDEHFRTAPLERNVPALMGLLN
                     VWYVNHLDARSHAVLPYAQQLHRFAAYLQQLTMESNGKSVRWDGTPVTTTTGEVFWGE
                     PGTNGQHAFYQLLHQGTQLIPADFIAVATPA"
BASE COUNT         1783 a         3979 c         3942 g         1722 t
ORIGIN      
        1 ctcgccggga cttcccagat caccctggtc ccacccagga ccgccatcgg cgacagcacc
       61 acgagcgacc cgcggcgccc ggcggcgcgc tcggagaggt tggcgaggcc gctgcgccgg
      121 cccgtctcga tcatcccgac gccatcgtcc tcgaccgtga cctgcacgag gtcggcctgc
      181 acgacgacag cgacgtcgac ctcgccggcc tgggcgtgcc gagccacgtt cgacagggcc
      241 tcacggatca cggcgagcag gtccaggcgg agccactcgt cgaccgtctc ggggtcgtcc
      301 aacggaccga cgacggtgag gcggggggtg aagcccaacg ggtcggcgaa gctcacgacc
      361 aggtcgtgca gttcttcgga caacggctgg gactcgggct ggtgcagggc gaagatcatc
      421 cgtcggatgt ctttgatcgc caggtcgagt tcgtccactg cctcgctcag acgcttctgg
      481 acatacgggt gggcggccac ccgcgacgcc gactgcaggc ccagacctgt ggcgaacaac
      541 cgctggatga ccacgtcgtg catgtcccgc gcgatgcgtt cgcgatcctc aaggacgacc
      601 atgcgggccc ggtcccgctg ggcgctggcc gcggtcagcg tgaccgccgc gaggtcggca
      661 tactgcttca acggctcggc cgcgtcgtat gccgtgtgcg actccccttc gccccacgcg
      721 acgacgagca gacccaggct gcccgtgccc acgctcatcg ggacgatggc cgtctggccg
      781 tgcgggggca gcccgctggc ttcgcgcaac tgggcggcca gcgagtcggc gaccgcctca
      841 ccttcgcgcg acaacagcaa cagcggctcg ccagagtcga ggatcgtcac ccagtgcggt
      901 tcggcgagag gggtgccgac gatgagacgc gaccgggggt cggtgccggt cgacgcctcg
      961 atgatcaact ggccgtcctc ccccgcgagc gcgaccgccg ccaactcggc ttcggcggcc
     1021 aattgggcac gctggacgag catcgagagg gccacgcgtt ggtcgcggcc gtaaagcagg
     1081 gcctgggtca tctcccgcag cgcgtccgac cactgtcgcc ccgaccgcgc catgtggaac
     1141 atccgggcgt tatcgatcgc catcccggct gcagcagcca gcgcgacgat gagttcctcg
     1201 tcctcgccgg tgaactccag accacccacc ttgtctgcga ggtagatatt gccgaacacc
     1261 tcgccgcgca catggatggg ggccccgagg aaggtcgtca tcaccgggtg tcccggcggg
     1321 aagccgacag cgtcggggtg ttcgccgacc gcggacagcc gcaacggtcg ggggtcgcgg
     1381 atgagcgtgc ccaggacacc gtgtccgaac ggctgcgaag cgacccgacc ggcctcctca
     1441 ggcgtcaacc ccgacgtgac aaactccatg agcccttcac cgtcggggcg cagcacaccc
     1501 agggcgccgt accgggcccc cacgaggtcg caagcgctac gcacgatccg agtgagcacc
     1561 tctgagagat ccagaccgct tccgaccgcc agcacggcgt tgagcaacgt cgtgacggca
     1621 tgtgaggagc gcggcatgct caagccggtg aggttcaact gcacgtaacg atgctaggcc
     1681 agtgcgctca ggcggcgctg atgggcaccg tgacgacggg gcactcggcg tgcgccacgc
     1741 accgccgggc gatggtccgc gccgtgccct cgtcggtgcc gacgatcagg gcggccgcgc
     1801 ctcgggtgag ttcgaggagg gtcggcgtcg ggtcgccggt gacgacctcg aagcgacacg
     1861 gtatgccgcg cagcccccgc agcgcgtgga cggcggctcg gaaggtggcc tggtctgcct
     1921 cggcccgctc gtccgggctc aggccctcct cgacgatctg gacgaaccgc aaccgcgcac
     1981 cccgccgaac tgcctcggtg acggcggcct tggccaccga ttctgcccgc ccgtcgttga
     2041 gcaggccggc aacgacgtct cccgatcgcc ccggcgtata cgggacgccc tgctgcgccc
     2101 tcgttgtgct cacggggcca tcgtccgacg tggctcaccg tcgaatcagg gcccaaagtc
     2161 cctcagcccg tgccggtccc ggcgtgtcgg ccgggagtgc cccacatgcc ccttgggaga
     2221 acgcttccag tcagggccga cgcggccgac tcctcgtcgg atgggtcgcc gcgtagacgg
     2281 ccgcctgggt gcggctgtcc atcccgagct tgtccaggat cgaggtgacg tagttcttca
     2341 cggtcttctc ggcgaggaac atgtcctcgg cgatctgccg gttggtgagg ccttcggcga
     2401 tgtgctcgag gatccgtcgc tcctgaggtg tgagcgaacg caaccgaggg tccaccgagg
     2461 actcccccag gctcgacttc acccgagcca tgacctcggg gcggatgagg tgctctccgg
     2521 cggccacccg acggatagcg tcaacgaggt cattgccctt gatgtccttg aggacgtatc
     2581 ccgaggcgcc ggcgagcacg gcggccttga gggcctggtc gtcgtcgtac gacgtgagaa
     2641 tgagcgcccg aagcgtcggg tcgacggccc ggaccgcgcg gcatacgtcg atgccggagc
     2701 cgtccggcag gcgggcgtcc aggacgcaga ccttcggccg caaggcggga atgcgtcgtt
     2761 cggcctccaa cgccgacgcg gactcgccca cgacctcgat gtcccctgag agttcgagca
     2821 gttcacgcag gccgcgccgg acgatctcgt gatcgtcgag caggaaaaca gtgatcgtca
     2881 cggggcctag ggaatcacac ttcccgggtg gtctgggggt cactgcgatt cgtaaaagag
     2941 cgactcggtc acggcacgcg cacggcggga cgcacgaagg tagtcctcca tcagcccggc
     3001 gcccgtccct gggtcgcggc caacgatccg ccccacccca tcggcgtcgc ggatgtcggt
     3061 gggaacggca tccccggggc gcccgcgcca caacaggttt gcgttgcgca accggctcgc
     3121 gttctcgtat gccgccgcca acgtcgcgct gtcttcctcc ccgaccagcc cggctgcggt
     3181 agcggctcgc aggcccgcca tcgtcccggg ggtttgcagg ctggggacct catgggcatg
     3241 gcgcagttgg aggagttgga cggtccattc gacgtcggtg aggccgcccc ggccgagctt
     3301 gagatgggtg cgtgggtcgg cgccgcgcgg cagtcgttcg gactccaccc gtgccttcat
     3361 cgtccggatc tcgcgcacct gcgtggcatc caacccggct gcgggatacc tcagagggtc
     3421 gatcagcgcg gcgaaggcct cccacaggcc ccggtccccg gcgatcggcc gggcccgcag
     3481 caacgcctgg aactcccagg tgagggccca ccggtcgtag tagacggcat acgaggcgag
     3541 gctgcgggtc aggggcccgt tcttgccctc cggacgcaga tcggtgtcga ggacgagcgc
     3601 atccgacccg gtcgccgcca cgagccgcac gagttcttgg acgatggcca aaccctgccg
     3661 atgggcggct tcgtcggaca ccccgggccg gggctggtgg atgaccatga catcggcgtc
     3721 ggacgcgtag cccaactcgc gcccgccgag gcttcccatc ccgacgacga gcacgtccac
     3781 gagcaacccg tcctcgcccc caccgacgac ccgggtcgcg acctccaggg cggcctcgac
     3841 ggtcgctccg gcaaggtcgg cgagagcagc cccgacctgg tccgcggcga attgaccggt
     3901 gagatcgccg aagacgatcc gcagcagttc gtgccgccgc aatgccccga ccgcagccat
     3961 cgccttgtcg gggttgtcct tgcgagcagc cgcgctgcgc aacgtgatcg ccagggactc
     4021 ctgactgcgt ggccgcagcc cgtcggggtc gccgaggatc gacaccgact cgggagcgcg
     4081 gatgaggagg tcggcggcat acctgctgcg tccgaggacg tgcgcgagcc gctcggcggc
     4141 actgccttcg tcgcggagca tcttgaggta ccagtggatt gagccgaggt cgtcgctcac
     4201 ccggcggaag gcgagcaacc cggcgtcggg gtcggcctca tcggcgaacc acgagagcat
     4261 caccggcagg agggtgcgct ggatcgccgc acgccgcgag acccccgcgg tcaaggtctc
     4321 caggtggcgc atggcgccgg cggggtcgcg gaagccgagg gcggcgaggc gctcgcgagc
     4381 ctgctggggc gacaggctcg tgtcgtcgct gctcaaccgc gcggcggcgg ccaggagcgg
     4441 ccggtagaag agccgttcgt ggatgcggcg cacctcgcgt gcctgcgact gccacatcgc
     4501 gacgacggcc tcggctggct tgctggtcag acccaacgcg cggccgagga cgcgcaactc
     4561 gtcgggagcg gtcggcatga ggtgggtgcg gcgcagccgg tagagctgga tccggtgttc
     4621 caaggtccgc agcagccggt atgccgcgtc gagggtcgcc gcgtcttcgc gcccgacgta
     4681 gccccccgag gccagggcgt gcagggcctg caaagtcgtc gccgaacgca ggctctcgtc
     4741 gtgtcgaccg tggacgagtt ggagcagttg caccgagaac tcgacgtcgc gcagcccgcc
     4801 cgggccgagc ttgagttggc gggcggcctc ggcggccggc acatgttgct cgacgcgacg
     4861 gcgcatcgcc tggacgtcct cgacgaagtg ctcgcgcccg gcggcgcccc agatcatcga
     4921 ccgcagggcg tccttgtagg cgatgccgac ctcacggtca ccggccgaga cccacgcctt
     4981 gagcaatgcc tggaactccc aggtcttggc ccagcgctcg tagtaggcct tgtggctggc
     5041 gacggtccgc acgagcgggc cggccttccc ttccgggcgc agggcggcat cgaccggcca
     5101 cagggtgccc tcgccggtct gggccgcaca ggccttcatc gtcgccgtcg cgagcgccgt
     5161 cccgaccttg atcgcgcgtt cctcatcgac cccgtcggcc ggctcgacga cgaagatgac
     5221 gtcaacgtcc gagacgtagt tgagctcccc gccgccggtc ttgcccatcc cgatgatggc
     5281 cagccggcag tcctcgtgac cgggcccgaa ctcctcgcgg gcgatgtcga gggcggcctc
     5341 cagcgcggcc tcggccagtt gggcgagcgc ctcggcggcc gctggcaggt gcgaaaccgg
     5401 gtcgggatcg gacagatcca aggccgcgat ccccacgagt tcacggcgat aggccacccg
     5461 caacgcgtcg tatgccgttg cggcacctcg cgcctgcgga gtcacggccg tcgtcagcgc
     5521 agcccgcagg cccggcccgg tcgggggggt cgcgtcggtc gcagcgaccc aatgctccgg
     5581 gtgggcagcg aggtgatcca ccagtgcccg cgacccaccg agggtcagca gcagccgtcg
     5641 gcgccggacg gagtcggtgc tgagaacctc agcgagagcg cgtgggtcgc tggggtgcgc
     5701 gggcggcata ccgaggacgg cctcgaccag ccgcaccagc ccgagcgcgg cgaggtcggg
     5761 gtcggctgct gtggccagcc cttcagcgac ggaggtgcgg accgcgcggg tgctctcgat
     5821 gagcgcgtcg gtgtcgagcg tcgcgccggc gcccgggtca cccccgggag cgaacaggcg
     5881 caacaggacg gcgtcacgca gcagccccgc cgaccgctcg ggctcggcga acccgagccg
     5941 agccatcagg gcgacagcgc tggaggaccg atcgaccacg acgcgctccc cttcttgacg
     6001 gtcctagagc cgcgtgaggt agcggtccaa ctcgaactgg gtgatctgct gacggtactc
     6061 ccgccattcg gcccgcttgt tacgcaggaa gaagtcgaag acgtgctccc ccagcgtctc
     6121 ggcgaccaac tcgctgcgct ccatgagctc cagggcgcga cccagcgacg acggcaacgg
     6181 ctcgatgccc atggcccggc gctcggagtc ggtgaggctc cacacgtcgt cctctgcctc
     6241 tggaggcaac tcgtagccct cctcgatgcc tttgaggcca gcggcgagga gcaccgcgag
     6301 cgcgaggtag gggttgcagg cggagtccag cgagcggacc tccacgcggg tcgagttgcc
     6361 cttgtcgggc ttgtacatcg ggacgcgcac gagcgcggat cggttgttgt gtccccaggt
     6421 gaggtatgcc ggtgcctcgc cgccgcccca caaccgcttg taggagttga cccactggtt
     6481 ggtgacggcg gtgatctcgg cgccgtggcg cagcagaccg gcgatgaatt gccgcccggt
     6541 ctttgacaat tggtagggag cgccggcctc gtggaaggcg ttggtgtcgc cctcgaagag
     6601 gctcaggtgg gtgtgcatgc ccgacccggg gtgctcgcgg aacggcttgg gcatgaacga
     6661 cgcccggacg ccctgctcaa gagcgacctc cttgacgacg gtgcggaagg tcatgatgtt
     6721 gtcggccgtc gagagggcgt cggcatagcg caggtcgatc tcgttctgcc cgggcgcgcc
     6781 ctcgtggtgg ctgaactcca ccgagatgcc catgttctcc agcagcgtga tcgcggcccg
     6841 gcggaagtcg tgggcggtgc cgtgtgggac gtggtcgaag tagccgccgt cgtcgaccgg
     6901 cacgggggtg atccgcttgc ccttcggcgg gtgcagcagg aagaactcga tctcggggtg
     6961 ggtgtagaag gtgaacccca agttggcggc ccggctcaag gcccggcgca ggacgtggcg
     7021 cgggtcggcg aaactgggct gcccgtcagg ggtgagcagg tcgcagaaca ttcgcgcggt
     7081 gcccgggttc tccccgcgcc acggcagcac ctggaaggtc gacgggtcgg gcttgacgag
     7141 catgtcggcc tcatagacgc gggcgaagcc ctcgatggcc gagccgtcga agccgatccc
     7201 ctcggcgaag gcaccctcaa gctcggcggg cgcaaccgcc accgacttca acgtcccgac
     7261 gacgtcggtg aaccacaacc ggacgaaccg gatgtcgcgc tcttcgatcg tgcgcaggac
     7321 gaactcttgc tgacggtcca tgtgacgatc ctgccgcacc ggtgttaccg gcgcgtttca
     7381 ggccccgact ttggtcagtc cagggccagt accggatgag cccgggatca gtcctcgagt
     7441 tcctcgtcgt cccacgtcgt ggtggcgcca gattcgttcc acgccttgtc ctcggcgtcc
     7501 cacttctcgg tgcgctcgtg ggcgctctcc agggcgcggg ccgcttcgtc ggcagtgtcg
     7561 tacgggccca gcacgtcctc gcccggaccg cggtcatcgt cgttctccac gacgcccttg
     7621 gccacgttgt accagtactg cacggcatac ctcccggtct tgtgggaacg acagacacac
     7681 cgtatgccgt ccccgcaact tcgcgcgcgt gccccctgtc ttcgcgcaga tgcatagact
     7741 ccgcggtatg ccggtgagct tcgcgagcgt cgcgccgcac ccagtcggcc cgcgccgcag
     7801 cgtccctgtc ggcatcccta ggccggagta cgtcgacaag cctgccccag cgcggtatca
     7861 ggggtccgag gtccacgacg ccgagatgat cgaacggatc cgggtggccg ggcgagtggc
     7921 tgcgcaggcg atggcggctg ccgccgcggc gatcgcgccc ggcgtgacga ccgacgaact
     7981 cgaccggatc gggcacgagt tcctgctgga tcacggggcc tatccctcaa cgctcggcta
     8041 ccgcggattc cccaagtcgt tgtgcacatc agtcaacgag gtcgtgtgtc acggcatccc
     8101 cgattcccgg cggctgcagg acggcgacat cgtcaacatc gacatcacgg catacctcaa
     8161 cggggtccac ggagacaccg acgcgaccta ccccgtgggc gaggtcgacg aggagtcgcg
     8221 gctgctcatc gagcgcaccc acgaggcgat gatgcgcggg atcaaggccg tggcgccggg
     8281 tcgaccgatc aacgttatcg gccgggtgat ctcttcctac gcaaggagat tcggctacgg
     8341 ggtcgtgcgc gacttcaccg gccacgggat cgccgaggcc ttccacagcg gcctgatcat
     8401 cccccactac gacgccgcgc cgtcctacgc caccgtcatg caaccgggca tgacgttcac
     8461 catcgaaccg atgctcaacc tcggcaccca cgagtgggac atgtgggacg acgggtggac
     8521 cgtcgtcacg cgggaccggc gccgttcggc gcagttcgaa cacaccctgg tggtcaccga
     8581 gacgggtagt gagattctca ccctcccgta gacaagctcg cgcccccata gggaagtatg
     8641 tgcccatgac ctcgcgtgtg gccctcggaa tcgacatcgg cggatcgggc atcaagggag
     8701 ctccggtcga cctcgaccgc ggggtgttgc tcgcagaccg agtgaagatc gagactccgg
     8761 cggagtccac gccccaagcg gtcgcggaga tcgtccgcca gatcgctacg catttcatcg
     8821 acaccctccc cgctgacgcg ccgatcggcg tcaccatccc cggggtcgtg cagcacggcg
     8881 tcgtgcgcac ggctgccaac atcgacaagg cctggctcga ctgcccaggc gaggcgctgt
     8941 tcagcgccac gttgggtcgg cgttgcctgc tggtcaacga cgccgacgcc gcgggtgtcg
     9001 ccgagttgcg ccacggcgcg gccaaggacg tcgaaggttt ggtgatcgtc acgaccctcg
     9061 gaaccggcat cggtatcgcc ctgctgcaca acggcgtgct catccccaac gcggaactcg
     9121 gccaccttga gctcgacggc cacgacgccg agacccgcgc cgcagccagc gctcgcgagc
     9181 gtgaggggtt gtcctggaac aagtgggccg ggcgtctgca gcgctactac acccacctgg
     9241 agaacctcct ctggccggac ctgttcgtcg tcggcggcgg ggtctcgcgc aagtcggaga
     9301 agttccttcc gttgctgtcg ctgcgcacgc ccatcgtccc ggccgagttg cagaacgccg
     9361 ccggcatcat cggcgctgcc gctctcgccg ccgactgagg ccgctacggc ggaactgtta
     9421 agggatggag actgcgcctc gcccggcgag gtcgtgcagg gtgaggtcat cgccgccgtt
     9481 ggtggcgata tgggtgagat agagggctcg tccgggcgga gcgactaacc cgtcgtggat
     9541 cgggaccatc gagcgcggag cgagccggcg cacgaagtcg atggagtctc ggacggcaca
     9601 ccacggggcg ttgatcggca cggccaacac gtcgatgtct ccgggctcgc cgtcgtaggc
     9661 atcgcccggg tggaacaggc tcggctcccc cgccgcgtca aggcgcaccc cgacgttggc
     9721 gacggtcggg atccattgat ggttgaacgc gtgcagcgcc cccaccgggc gcacgcggac
     9781 gtcgccgagg tgcaggtcag cgccctgcgc gagcactccg acggccagcc cggcctcgac
     9841 cagcaacgcc gcactcatcg ggtcggcatg caccgcggcc ccggggttgg cccggaccag
     9901 tgccggaagc ctcttctggt cgaggtggtc ggcgtgttgg tgggtgacga ggatcgcatc
     9961 caggtcggtg aggtcctcga aacccgacga gaagccgccc gggtcgatga ggatgcggcg
    10021 atcggccaac tcgacgaaga ggcacgagtg ccccaggtgc gtgatgcgca tgacggaaac
    10081 gctagccgga cgcacggcat acctggccgc aatagggttg gggactgagg cgaacccacc
    10141 ccggaaggtg atcgacatga ctgcgccggt agacccgacg acgaccgcgg cctggagccg
    10201 actgaccagc gcggcgacca cattggagcc ggaccttcgt gcctggtttg ccactgatcc
    10261 cacacgggcg caacggtttt cgttcgagtg cgccgacctg cacgtcgacc tgtcgaagaa
    10321 cctcctgacc gacgacatcc tcgctgccct cgtcgagctc ggccgcgagg tcgggctcga
    10381 ggagcgtcgc ggcgcgatgc tcgccggaga gcggatcaac gtcacggaag gccggtcggt
    10441 gcttcacacc gcgctgcgtc gcccggcgga cgcggcaccg ggcctggtgc tcgacgggca
    10501 ggacgtcgac cacgacgtcc acgaggtgct cgccaaggtc tatgccttcg ccgaccgggt
    10561 gcgctcgggc gactggcgcg gcgtgaccgg caaacggatc gagacgatcg tcaacgtcgg
    10621 gatcggcggc tcggacctcg ggccggtcat gatctacgag gcgctggcgc cctatgtcca
    10681 agccgggctc acctgccggt tcatctccaa catcgacccg accgatgcgg cccagaagac
    10741 ggccggtctg gaccccgaga cgacgctcgt catcgtcgcc agcaagacct tcaccaccct
    10801 cgaaacgatc accaacgccc gcctcgtgcg cacctggttg ctcgactcac tggcagcgtc
    10861 tggcgcgatc gacgggtcgg acgcctcgca ggccgacgcc gtcgccaagc acttcgtcgc
    10921 ggtgtcgacc gccctggaca aggtcgctgc cttcgggatt gacccggcca acgcgttcgg
    10981 cttctgggac tgggtcggcg gtcgttactc cgtcgactcc gcgatcggca cggctgtcgc
    11041 ggtggctatc gggccggagc ggttcgccga gttgctcgcc ggcttccatg cggtcgacga
    11101 gcacttccgc accgcgcccc tggagcgcaa cgtgcctgcc ctcatgggcc tgctcaacgt
    11161 ctggtacgtc aaccacctcg acgcccgcag ccacgcggtg ctcccctacg cccagcaact
    11221 gcaccggttc gcggcatacc tgcagcagtt gacgatggag tccaacggga agtcggtccg
    11281 ttgggacggc accccggtga ccaccaccac cggagaagtc ttctggggcg agcccggcac
    11341 caacgggcag cacgcgttct accaactgct gcaccagggc acccagctca tcccggcgga
    11401 cttcatcgcc gtggccacac cggcat
//
LOCUS       DAORPW010000073        11103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1315_length_11103_cov_780.193519, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000073 DAORPW010000000
VERSION     DAORPW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1315_length_11103_cov_780.193519"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..4577
                     /locus_tag="PLB86_07455"
     CDS             186..4577
                     /locus_tag="PLB86_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HRC65012.1"
                     /translation="MRFSVVVRDRSAGTEAVVLVDADPDATVAALLPALLGAVGSAIH
                     PGFASKVPVWVDGRPVQPEDTARESGLAAGSVVCLHENDATPSTLPTGIVEVRVVSGP
                     GAGRIHRARLGETVIGCGANGLSLPDPLLPADALTLTVARDGTATVQPADGIAATLDG
                     TDLEGATQWPPGATLSAGQTLLTLEAPWQPDALTVAADGRLGRDVNRPPRLLPAPWIR
                     EFTLPHKPEKPTRMGIPWLMVLSPMLMAVPMVWLMGSWRFAAFALLSPIMAIFNLIQG
                     RRSGAADYKRKLAAYESTLVKVRERAAEALARERTERRAALPDPAQVLLQAVGPGARL
                     WERRRTDPDYLVLRVGTATRASQVRVEDPNVDRNAEDVAPATVADVPVPVALRDFGVL
                     GVTGAADQVDATARWLIGQAAVLHSPRDLRLVVLTDQRGEDRLGWVRWLPHTRTDDET
                     LPAFVGNEQQSIGARVGELTRLVTDRVAAAEEGFGAGAKEMTGPDYLVVLDGARLLRA
                     LPGVVTILRQGPAVGVRVICLDPDRRSLPEECRVVLECSDDESLVTATGVADVPEVRL
                     DLVETAWAARVAQALAPLRDITPSLEDAGLPRSARLLECVGLTDPTPDAVVAGWGQPQ
                     TDVVIGAGFDGMFRIDLRRDGPHALVAGTTGSGKSELLQTIVASLALANRPDQLTFVL
                     VDYKGGSAFKECNKLPHTVGMVTDLDTHLVGRALTSLGAELRRREHLLSVPGAKDLED
                     YWALCRNDPTLPTIPRLVLVIDEFASLKAELPDFVTGLVTIAQRGRSLGIHLILATQR
                     PSGVVSADIRANTNLRIALRVTDESESRDVIDAPEAGRILASTPGRGYARLGPSGVLP
                     FQAGRVGGRRPDAPSETRTIPTPLAWGIPWSRTGIPAPGRPTTAASDTDEGETDLSVL
                     VTAVRGAVDHLGMPPQHSPWLPALPGFVTLDELRARLVERDPAAAADPFTVGWALEDR
                     PEDQAQVPATFTLGTSGHLFLVGGPRSGRSTALRTLVGALAEKVAARDLHVYGLDCGN
                     GALLPLTALAHTGAVVRRTEVERASRLIRRLTEEVARRQSVLGAHGFADIQELRSAAA
                     PDDRLPYVLLVLDRWEGFMSDLADVDVGSLLDGMVTLLREGASVGIQVVVTGDRTLNS
                     ARVASLVEAKLALRLPDRQDYASLGLKVRDLPEEFPEGRGVWGERSIEAQIAVLGADA
                     SGQGQGTSLRELAAATAERDRDLPRALRPMALGALPDQVAWADVAADFDPGARPPSWL
                     PFGIGGDLVEPIGLELDRSPVALVAGESRTGKTATLCFLAAYARRSGRAVLGFVPRDN
                     DLSADLGEGACIVGQEDPNAAVARLRDLPEGALVLVDDVEAYKDAPLTPLMNALVRQA
                     ADRGFTVVLSGRSADLGAGYSGWMFEARRGRHGLVLSPSEAIDGELFGSRVARTALRS
                     RPHAGRGVLFDSTGAQTTVQVPLVERG"
     gene            complement(4637..4936)
                     /locus_tag="PLB86_07460"
     CDS             complement(4637..4936)
                     /locus_tag="PLB86_07460"
                     /inference="COORDINATES: protein motif:HMM:NF017793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HRC65013.1"
                     /translation="MAVGDYVKINHGDIEQQATALAAMKNELEENFKRAQAQVVSLNE
                     SGAFQGLSGSSFTEEYAKWTTSITTTLQLMEQFGQHLGKTSKAFAEVDAAFSLKG"
     gene            5371..5961
                     /locus_tag="PLB86_07465"
     CDS             5371..5961
                     /locus_tag="PLB86_07465"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HRC65014.1"
                     /translation="MIGLAVLLIVGSAAIAGLLAMRIDSRVPVLVAAHEIPVGAQITL
                     ADLATQRVASDGLEVLPATAADQLVGKYAAGTIPAGRLLDQAMFAQTGFLRDGAVAVG
                     VSLAPGRMPASGLRAGDRVQVVDVKDGVGVVLVENATVSSAPDTTSSSGAFGGGSTGG
                     SGSVVATLIVPPSSAPAVAASSAANRIAVVLLRRGA"
     gene            5965..6747
                     /locus_tag="PLB86_07470"
     CDS             5965..6747
                     /locus_tag="PLB86_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65015.1"
                     /translation="MALYAVCSAKGSPGVTTLVAAMAMAWDRPVVMADLDAGGGDLAL
                     RYRDERGRPLDQDLGLVSLAALSRRSTTTTPIGPHLQVPLGGPPILVGVARPEQVGAL
                     GPAWGPLAQSLAAYEGDVLADCGRVTAGAAVTPVLAAADALLFVVRPTMEELYHLRER
                     LGGLAEQLGSRDIHGRRIAVAVVGSDRNRTGASDVQRLLDASGSGATVVGVFVEDAKG
                     ALRLRYDLAGVPARSVVLRSAGVVGSRVRELAAHDLTAATGV"
     gene            6750..8045
                     /locus_tag="PLB86_07475"
     CDS             6750..8045
                     /locus_tag="PLB86_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRC65016.1"
                     /translation="MTDQLLLRRLREDVADIVSRQRREDAANGVPPMSSEDERQFGRA
                     VISRVLETHARTEMAGGRTPPTMEEEAELADGIHAALFGVGRLQPLLDDPMVENVDIN
                     GCDRVFVGYADGREEMVAPIAESDEELIELVQVLAVYSGLTSRPFDTANPQLDLRLPD
                     GSRLSAVMGVTARPSLSIRRARLSRVFLDDLVVNGSMSSEIAEFLSAAVRARKNIMIA
                     GATNAGKTTLLRALANEIPRNERLITVERAMELGLGQFQDLHPNVVAFEERLPNSEGQ
                     GHIGMAELVRRSLRMNPSRVIVGEVLGDEIVTMLNAMSQGNDGSLSTIHANSSLEVFN
                     RICTYAIQSVERLPADATMMLIAGAIDFVVFVERRNEFHSGGGMRRIITSIREVNGVD
                     GRVLSSEVFAEGSDGVAVPAAAISCIDDLLAAGYSGMSR"
     gene            8071..8958
                     /locus_tag="PLB86_07480"
     CDS             8071..8958
                     /locus_tag="PLB86_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRC65017.1"
                     /translation="MFAQLLVPLVVLLGGLCGVGILLLVKGLTPTEVSLRPRSTLAAD
                     LRRMSIMAGRKVPVAVGAGLFTLVITRWIVLAVSVGLVVFFWGALFGGAAAERAAYER
                     LEGLAAWTESLRDTIAGAVGLEQAIPATANAASPPIARAINDLSDRLRVRVPMTTALM
                     RFADDLDDSTADLVVAALILNAKLRGPGLRDVLSSLASSVRSEVEMRGRISAGRRGTR
                     KSVQIVVAITGLFVIGLRVLNPDYVEPYGTPLGQLVLLLVAGIFALGFYWLRRLATFQ
                     MPARFLQAPPTDVTSGAPR"
     gene            8955..9878
                     /locus_tag="PLB86_07485"
     CDS             8955..9878
                     /locus_tag="PLB86_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRC65018.1"
                     /translation="MTLTLVLIAGAICGVGILALVLLLDVRPARGPAALARLDLDRRR
                     FRREAVLEVDALHAYESMRLRRVGAALRESLEARGIRLPAGLQADLAITGRSVENHLA
                     ISLASGLAGLMLPLLLVAPFAAFFGLGMGLVPLWIALLLGALGAILPSLSVQSRAADL
                     RRDFRHVVSSFLDLVAMNLSGGRGVPEALEVASSVSTYWGMSRIREKLRNARLQGVTP
                     WSALGELGQELAIDELRDLASALTLVAEDGAKVRDSLFARASSMRQRELADAEGRAAA
                     RSQSMVVAQMLLAIGFLTFLIFPAVARLLGS"
     gene            10030..10248
                     /locus_tag="PLB86_07490"
     CDS             10030..10248
                     /locus_tag="PLB86_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65019.1"
                     /translation="MYVKSVGAAHYLEAALRARLGHARADRGASVIEWVIISAVLVIL
                     VGVVGGILYTKINSAANAVNLAPAVGGP"
     gene            10211..10663
                     /locus_tag="PLB86_07495"
     CDS             10211..10663
                     /locus_tag="PLB86_07495"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HRC65020.1"
                     /translation="MRSTSLRPSVAPDRRRGDAQPHGGRRERGSSVIELTLYMPILML
                     VVFVAVQFSLTYLGNQVVSSVARETARAVRQGADTGQANAVAVDYAQKIGSGVISNLS
                     VTVTHPDADTVRVRVAADTLHLVSISSLSRVSAEVQGPMETFRPDTGP"
     gene            10698..11072
                     /locus_tag="PLB86_07500"
     CDS             10698..11072
                     /locus_tag="PLB86_07500"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRC65021.1"
                     /translation="MAIEVIVLVPILFSIIMLIVAGGRLVQRQGQVDSAARDAVRAAS
                     MERSIDAARTAASQTANVTLGGQPCQTADLSGSDFRAGGLVRVRLTCTVSYADLGLVG
                     LPGQTTVTSVSTAPLDIFRRAG"
BASE COUNT         1484 a         3878 c         3930 g         1811 t
ORIGIN      
        1 gtaggtcccc tgagcccccg acaatagcga cgctcggaca agcgccttat ccgcacgcgt
       61 actcaggtgg acggcatacc tccgcgtccg tgaccccgtc cgcacggggg ctgcgggacg
      121 tgcgggcgcg actccggtac ggtccagggg tcggcccagc ggtggcggcg tcagggaggt
      181 atgccgtgcg gttcagtgtg gtggtgcggg acaggagcgc cggcacggaa gccgtcgtgc
      241 tcgtcgacgc cgacccggac gccaccgtcg cggcgttgct gccggcgttg ttgggcgcgg
      301 tcggcagcgc catccacccc ggcttcgcca gcaaggtccc cgtgtgggtc gacggtcgcc
      361 ccgtccagcc ggaggacacg gcgcgggagt cgggcctggc ggccggctcg gtcgtgtgtc
      421 ttcacgagaa cgatgcgacg ccatcgaccc tcccgaccgg catcgtcgag gtccgcgtcg
      481 tctcggggcc gggcgcgggg cggatccatc gggctcgcct cggggagacg gtcatcgggt
      541 gcggggcgaa cgggttgtcc ctgcccgatc cgctgctgcc ggccgacgcg ctgaccctca
      601 cggtggcccg cgacggcacc gcgaccgtgc aaccggccga cgggatcgcc gcgaccctcg
      661 atggcactga cctcgaaggc gccacgcagt ggcctcccgg agcgacgttg agcgccggcc
      721 agaccctgct cacacttgag gcgccctggc agcccgatgc cctgaccgtg gcagccgacg
      781 gccgcctggg tcgcgacgtc aaccggccgc cgcgcctgct cccggcaccg tggatccggg
      841 agttcaccct gccgcacaaa cccgagaagc ccacgcggat ggggatcccg tggctcatgg
      901 tgctctcgcc gatgctcatg gccgtgccga tggtgtggct catgggtagc tggcggtttg
      961 ccgcgttcgc cctcctctcg ccgatcatgg cgatcttcaa cctcatccag gggcggcgtt
     1021 ctggtgccgc cgactacaaa cgcaagctcg cggcatacga gagcaccctg gtgaaagtcc
     1081 gcgaacgggc ggccgaggcg ttggcgcggg agcgcaccga gcggcgcgcg gccctgcccg
     1141 acccggccca agtgctcctg caggcggtcg gtcccggcgc tcggctgtgg gagcgccgcc
     1201 gcaccgaccc cgactacctc gtgctgcgcg tgggcacagc aacccgcgcc agccaggtcc
     1261 gcgtcgagga tcccaacgtc gaccgcaacg ccgaggacgt ggccccggcg acagtggccg
     1321 acgtgccagt ccccgtggcg cttcgcgact tcggcgtgct cggagtgacc ggagccgccg
     1381 accaggtgga cgcgacggct cggtggctca tcggccaggc agcggtcctg cacagtcccc
     1441 gcgacctccg cctggtggtg ctcaccgatc aacgcggcga ggaccgcctc ggctgggtgc
     1501 gctggctgcc gcacacgcgc accgacgacg agacgcttcc ggcgttcgtc ggcaacgagc
     1561 agcagtcgat cggggcccgg gtcggcgagt tgacccggtt ggtcaccgat cgggtcgccg
     1621 ccgccgagga gggcttcggc gcgggggcca aggaaatgac cggcccggac tacctcgtcg
     1681 tcctcgacgg tgcccggctg ttgcgggcgc tgccgggcgt cgtgaccatc ctgcgccaag
     1741 gcccggcggt cggagttcgg gtgatctgcc tcgaccccga tcggcggtcg ctgcccgagg
     1801 agtgccgggt cgtgctggag tgctcggacg acgaatcgct ggtcaccgca acaggggtcg
     1861 cggacgttcc cgaggtgcga cttgacctcg tcgagacggc ctgggcggcg cgggtcgcgc
     1921 aggccttggc tcccttgcga gacatcacgc cgtcgctgga ggatgccggc ctgccgcgct
     1981 cggcccggtt gttggagtgt gtcgggctga ccgatccgac tcccgatgcc gtggtggccg
     2041 gatgggggca gccgcagacc gacgtcgtca tcggtgcggg cttcgacgga atgttccgga
     2101 tcgacctgcg ccgcgatggg ccgcacgcgc tggtggccgg gacgacgggc tcgggcaagt
     2161 cagagttgct tcagaccatc gtcgcttcgc tcgccctggc caatcgcccc gatcagttga
     2221 ccttcgtgct cgtggactac aagggcgggt cggcgttcaa ggagtgcaac aagcttccgc
     2281 acaccgtcgg catggtcacc gacctcgaca cgcacctcgt cggccgggcg ctgacctccc
     2341 ttggtgccga gttgcgtcgg cgcgagcacc tgctcagtgt gccgggggcc aaggacctcg
     2401 aggactactg ggcgttgtgt cgcaacgacc cgaccttgcc gacgatcccg cgcctggtcc
     2461 tggtcatcga cgagttcgcc agcctcaagg ccgagttgcc cgacttcgtc accgggctcg
     2521 tcaccatcgc ccaacgcggc cgatcactgg ggatccacct catcctcgcc acccagcgcc
     2581 ccagcggtgt cgtgtcggca gacatccggg ccaacaccaa cctgcggatc gccctgcggg
     2641 tcaccgacga gagcgagagc cgcgacgtca tcgacgcacc ggaggccggg cggatcctcg
     2701 cctcgacccc cgggcgcggc tacgcccggc tcggtccgtc gggcgtcctg cccttccagg
     2761 ccggccgagt cggcggccgg cgacccgacg ccccgagtga gacccgcacc atcccgacgc
     2821 cgctcgcatg ggggattccg tggtcacgca ccggcatacc ggcgccgggt cggcccacca
     2881 cggcggccag cgacaccgac gaaggggaga ccgacctgtc ggtcctcgtc accgccgtcc
     2941 gtggggccgt cgaccacctg ggtatgccgc cccagcacag tccgtggctg cccgccctgc
     3001 ccgggttcgt caccctcgac gaactccgtg cgcggttggt ggagagggat ccggccgccg
     3061 cggccgatcc cttcaccgtc ggttgggcgc tggaagaccg gcctgaggat caggcccagg
     3121 tgccggcgac cttcaccctg gggacgtcgg gacatctttt cctcgtcggc gggccccgct
     3181 caggacgatc caccgcgttg cgcacgttgg tcggcgcgct ggccgagaag gtggcggcca
     3241 gagacctgca cgtctacggc ctggactgcg gcaacggtgc cctgttgccc ctcaccgcgt
     3301 tggcccacac gggggcggtc gtgcgccgaa ccgaggtgga gcgcgccagc cggttgatcc
     3361 ggcgattgac cgaagaggtc gcccggcggc agagcgtgct cggagcccac ggcttcgccg
     3421 atatccagga actgcgtagt gccgcagcgc cggacgatcg gttgccttat gtgctgctcg
     3481 tgctcgaccg gtgggagggc ttcatgtccg acctcgccga cgtcgatgtg ggcagcctcc
     3541 tggacggcat ggtcacgctg ctgcgcgagg gcgcatcggt cggcatccag gtcgtcgtga
     3601 cgggcgatcg gacgctcaac tcggcccggg tcgcctcgct ggtcgaggcc aagttggcct
     3661 tgcggctgcc tgatcggcag gactatgcca gcctcgggct caaggtgcgg gacctgcccg
     3721 aggagttccc cgagggtcgt ggcgtctggg gcgaacgctc catcgaagcc cagatcgccg
     3781 tcctcggcgc ggacgcgagc ggccagggtc agggaaccag cctgcgcgag ctcgcggcgg
     3841 caacggccga gcgcgaccgc gacctgccac gcgccctgcg tcccatggcg ttgggcgcgc
     3901 tgccggacca agtcgcctgg gccgacgtgg ccgcggactt cgaccccggc gcgcggccgc
     3961 cgtcctggct cccgttcggg atcggcggcg acctggtcga gcccatcggc ctcgagctgg
     4021 atcggtcgcc ggtcgcgctc gtggcggggg agtctcgcac cggcaagacc gcgacgttgt
     4081 gcttcctcgc ggcatatgcg cggcggtccg ggcgtgccgt cctcggcttc gtgccgcgcg
     4141 acaacgacct gtccgccgac ctgggtgagg gcgcctgcat cgtcggtcag gaggacccga
     4201 acgcggcagt ggcccggctc cgagacctcc ccgagggggc cctcgtcctc gtcgacgacg
     4261 tcgaggccta caaggacgca cccctgacgc cgctgatgaa cgcgctggtg cgccaggccg
     4321 ccgaccgggg cttcacggtc gtgctctccg ggcgatccgc cgacctgggg gccggctaca
     4381 gcggctggat gttcgaggcc aggcggggtc ggcatgggct ggtcctgtcc ccgtcggagg
     4441 cgatcgacgg ggaattgttc gggtcacggg tggcgcggac ggcgttgcgg tcgcgtccgc
     4501 acgccgggcg cggtgtgctg ttcgactcca cgggagcgca gacgacggtc caggtgccgc
     4561 tggtcgagcg gggctgacgc acgaacgccc cggccggtcg gccggggcgt tcgacgtgcg
     4621 gaggtgaggc gttcgctcag cccttgaggc tgaaggcagc gtcgacctcg gcgaaggcct
     4681 tggaggtctt ccccaggtgc tggccgaact gctccatcag ctgcagcgtc gtggtgatcg
     4741 aggtcgtcca cttggcgtac tcctcggtga acgaggatcc cgacagaccc tggaaggcgc
     4801 cgctctcgtt gagcgagacg acctgggcct gcgcgcgctt gaagttctct tcgagctcgt
     4861 tcttcatggc ggcgagcgca gtggcctgct gctcgatgtc accgtgattg atcttgacgt
     4921 agtctccaac agccatgagt gttccctttc gagttggtat gtcgggaaca gccaaacccc
     4981 ctgagggcgt cggcaacgtc gccgaccagc tgaggttccc tttcgtcgag gggatgcctc
     5041 gacgacgacg acaccatgac acgggtccgg cgcatggtca atgcgtgctc ctacggatgt
     5101 acaaagtcgt cgggactagg tagacctggc ttgctgagta ggtacacctg ctcgctgagt
     5161 aggtactcct gcctagtgcg taggggcatg ggctgagcgc ctaaactgtg cggtcggtag
     5221 ccaaagaccg gcggtcatga ccgccacgaa tcagggggca gcaccatcga cgacgtcagc
     5281 acctcacgca cgcgggtcgc ggcgggagcg gccggggcgg ctcgcgccgt gccacgaccg
     5341 gtgtccgccg ctcgccggcg ccgcccagcc atgatcgggt tggcggtcct gctcatcgtc
     5401 ggttccgcag caatcgcggg cctgctcgcg atgcggatcg acagccgggt cccggtgctc
     5461 gtcgccgctc atgagatccc tgtcggggcg cagatcaccc tcgccgacct ggcgacccag
     5521 cgggtcgcca gcgatggcct ggaagtcctc cccgctactg ccgcagacca actcgtcggc
     5581 aagtacgccg cgggcaccat cccggcggga cggttgctcg accaggcgat gttcgcccag
     5641 accggcttcc tgcgggacgg cgcggtcgcc gtgggtgtct cgctcgcgcc cggtcggatg
     5701 ccggccagcg gcctgcgcgc cggagaccgg gtccaggtcg tcgacgtcaa ggacggggtc
     5761 ggtgtggtcc tcgtcgagaa cgccacagtg tcctcggccc ctgacaccac gtcctcttcc
     5821 ggggcgttcg gggggggctc gaccggaggg tccggcagcg tcgtcgcgac gctgatcgtc
     5881 cctccgtcct cggcacccgc ggtcgccgcc tcgtccgcgg ccaaccgcat cgccgtggtc
     5941 ctgctgcgcc gcggggcctg acccatggcc ctgtatgccg tgtgctcggc caaggggtca
     6001 ccgggcgtga ccaccctcgt cgccgcgatg gcgatggcct gggaccgtcc cgtcgtcatg
     6061 gccgatctgg atgccggcgg gggagacctg gcgctgcgct accgcgacga gcgagggcgt
     6121 cccctcgatc aggatctcgg gcttgtctcg ctggccgcgt tgtcgaggcg ctcgaccacg
     6181 acgacgccga tcggcccgca cctgcaggtg cctctcggag gcccgccgat tcttgttggc
     6241 gtcgcccgcc ctgagcaggt cggtgccctg ggcccggcgt ggggcccgct ggcccagtcg
     6301 ttggcggcat acgaaggcga cgtcctggcc gactgcggtc gggtcacggc cggcgcggcc
     6361 gtcaccccgg tcctcgccgc cgcggacgcg ctgctcttcg tcgtgcgtcc gacgatggag
     6421 gagctctacc acctgcgtga gcgcctcggc ggtctggccg aacagttggg ctcgcgggac
     6481 atccacggtc gacggatcgc cgtcgctgtc gtcggctccg accgcaaccg caccggcgct
     6541 tccgacgtcc agcggctgct ggacgcgtcg gggtccggag cgactgttgt cggggtgttc
     6601 gtcgaggacg ccaagggagc ccttcgcctg cgctacgacc tggccggggt tcctgcccgg
     6661 tcggtggtcc tgcgctcggc aggcgtcgtg ggctctcgcg tgcgcgagct ggccgcccac
     6721 gatctgaccg cagcgacggg ggtctgatcg tgaccgatca actcctgctc cggcggctgc
     6781 gcgaggacgt cgccgacatc gtctcccggc aacggcgtga agacgccgcc aacggcgtgc
     6841 cgccgatgtc cagcgaggac gagcgtcagt tcggccgagc cgtcatctcg cgggtgctcg
     6901 aaacccacgc ccgcaccgag atggccggtg gtcgcacccc tcccacgatg gaggaagagg
     6961 ccgagctcgc cgacggcatc catgcggccc ttttcggcgt cgggcggctg cagccgctgc
     7021 tggatgaccc catggtcgag aacgtcgaca tcaacggctg cgaccgggtg ttcgtcggct
     7081 atgccgacgg gcgcgaggag atggtcgcac cgatcgccga gtccgacgaa gagctcatcg
     7141 agctggtcca ggtcctcgcg gtctactccg gcctgacgag ccggcccttc gatacggcca
     7201 acccgcaact ggacttgagg ttgcccgacg gaagccgact ctcggccgtc atgggcgtca
     7261 cggcccgacc gtcgctgtcg atccgtcgcg cccgcctctc gcgggtgttc ctcgacgacc
     7321 tcgtcgtcaa cgggtcgatg agcagcgaga ttgccgagtt cctgtcggcg gccgtccggg
     7381 cccgcaagaa catcatgatc gcgggggcca ccaacgccgg aaagaccacg ctgctgcggg
     7441 ccttggccaa cgagatcccc cgcaacgaac gcctcatcac cgttgaacgc gcgatggagc
     7501 tcgggctggg tcagttccag gatctgcacc ccaacgtcgt cgccttcgag gagcggttgc
     7561 ccaactccga gggccagggt cacatcggca tggccgagtt ggtgcgtcgg tcgctgcgta
     7621 tgaaccccag ccgggtcatc gtcggggagg tcctcggcga cgagatcgtc acgatgctca
     7681 acgccatgag ccagggcaac gacgggtcgc tctcgacgat ccacgccaac agttcgctcg
     7741 aggtgttcaa ccggatctgc acgtatgcga tccagtccgt ggagcgactg ccggccgacg
     7801 cgacgatgat gctcattgcc ggcgcgatcg acttcgtcgt cttcgtcgag cgacgtaacg
     7861 agttccactc cggcggtggg atgcgacgaa tcatcacctc gatccgcgag gtcaacggtg
     7921 tcgatggccg ggtgctctcg agcgaggtct tcgccgaggg atccgacggt gtcgccgtcc
     7981 cggccgcggc gatcagttgc atcgatgacc tgctcgccgc gggttactcg ggcatgagcc
     8041 gatgacggcg ggccgacgcc gatgaacggc gtgttcgcac aactcctggt cccgctcgtc
     8101 gtgctcctcg gcgggctctg cggcgtgggg atcctgctgc tcgtcaaggg gttgacgccc
     8161 accgaggtct cgctgcgacc gcgcagcacc ctcgcggccg acctgcgtcg gatgtcgatc
     8221 atggccggcc gcaaggtccc ggtcgcggtc ggggccgggc tgttcaccct tgtgatcacc
     8281 cgctggatcg tgctggccgt gagcgtcggg ctggtcgtct tcttctgggg cgccctgttc
     8341 gggggagcgg cagccgagcg cgcggcatac gagcgcctgg agggcctcgc ggcgtggacc
     8401 gagtcgttgc gcgacaccat tgccggcgcg gtcgggctgg agcaagcgat cccggccacc
     8461 gccaatgcgg cgtcgccgcc catcgctcgg gcgatcaacg acctgtccga tcgactgcgg
     8521 gttcgtgtgc cgatgacgac cgccctcatg cgcttcgccg acgacttgga cgactcgacg
     8581 gcagacctcg tcgtggcggc gttgatcctc aacgccaaac tgcgcgggcc gggtctgcgg
     8641 gacgtgctct cgtcgctggc gtccagtgtg cgtagcgagg tggagatgcg cggccggatc
     8701 agcgccggtc ggcgcggcac ccgcaagagc gtgcagatcg tcgtcgccat caccgggttg
     8761 ttcgtcatcg gtctgcgggt gctcaacccc gactatgtcg agccctatgg cacccccctc
     8821 ggccagctcg tgctgctgct cgtcgcaggg atcttcgcgc tcgggttcta ctggctgcgc
     8881 cggttggcga ccttccagat gcccgcccgc ttcctgcagg ctccgcccac cgacgtgacg
     8941 tcgggagccc cccgatgacc ctgaccctcg tgctcatcgc cggagccatc tgcggcgtcg
     9001 gcatcctcgc gctcgtgctc ctgctggacg tccgaccggc ccggggaccg gctgccctgg
     9061 cccggctcga tctggaccgg cgtcggttcc ggcgggaggc cgtcctcgag gtcgacgccc
     9121 tgcacgccta cgaatcgatg cggctacggc gcgtcggtgc ggccctacgc gagagcctcg
     9181 aagcccgtgg catccggttg ccggctggcc tgcaggctga cctggccatc accggacgtt
     9241 cggtcgagaa ccacctggcg atctcgctgg cctcggggct cgccggactg atgctgcccc
     9301 tgctgctcgt cgccccgttc gcggccttct tcgggcttgg aatgggcctg gtgccgctgt
     9361 ggatcgccct gctcctcgga gccctcggcg cgatcctgcc cagcctgagc gtgcagtccc
     9421 gggccgccga cctgcggcgg gacttccggc acgtcgtcag cagcttcttg gatctggtcg
     9481 cgatgaacct cagcggcggg cgcggagtcc ccgaggccct cgaggtcgcg agttcggtgt
     9541 ccacctattg ggggatgtcc cggatccggg agaagctgcg taacgcgcgg ctccagggcg
     9601 tcaccccgtg gtcggctctg ggcgagctcg gccaggagct ggcgatcgac gaactgcgcg
     9661 acctggcgtc cgcgctgacc ctcgtggccg aggacggcgc caaggtgcgt gactcgctgt
     9721 tcgcccgcgc gtcctcgatg cgccagcgcg agttggccga tgccgaggga cgcgccgccg
     9781 cccgctcgca atcgatggtg gtggcgcaga tgctgttggc gatcggcttc ctcaccttcc
     9841 tcatctttcc cgccgtcgca cgactgctcg gcagttgagt gccgggggaa gacgggctca
     9901 ccgagcccgt caagtgccgc cagggcgcca gcgctcgaag cggtttcgca aggtcacact
     9961 gattcggggt aacctgaggc gttcggtcac gccgcaggtc attctcggca actgctgaaa
    10021 ggggacggca tgtacgtcaa gagtgtcgga gctgcgcact atctggaggc cgccctgcgt
    10081 gcccggctgg ggcacgctcg ggccgaccgg ggtgcctcgg tcatcgagtg ggtgatcatc
    10141 agcgctgttc tggtgatcct cgtcggggtc gtcgggggca tcctctacac gaagatcaac
    10201 tcggcagcga atgcggtcaa cctcgctccg gccgtcggtg gcccctgacc gtcggcgcgg
    10261 ggacgcgcag ccacacgggg ggcgtcgcga gcgagggtcc agcgttatcg agttgaccct
    10321 ctacatgccc atcctcatgc tggtcgtctt cgttgccgtc cagttctcgc tgacctacct
    10381 gggcaaccag gtggtgtcgt cggtggctcg cgagaccgcg cgtgcggtgc gccaaggtgc
    10441 cgacaccggt caggcgaacg cggtcgcggt ggactacgcc cagaagatcg gttccggggt
    10501 gatctccaac ctgtctgtga cggtcacgca ccccgacgcc gacacggtcc gtgtccgggt
    10561 cgccgctgac acgctgcacc tggtgagcat ctcttcgctg agccgggtga gcgcggaggt
    10621 ccaggggccg atggagacct tccgcccgga cacagggccc tgacgtgcgc gcgggtcgtg
    10681 gtcgggagcg cgggtcgatg gccatcgagg tcattgtcct cgtgccgatc ctcttctcaa
    10741 taatcatgct catcgttgcg ggcgggcgcc tcgtccaacg ccagggccag gtggattccg
    10801 cggcacgaga tgccgttcgg gcggcgtcga tggagcggtc cattgacgcg gcgaggacgg
    10861 cagccagcca gacggccaac gtcaccctgg gcggccaacc gtgccagacg gccgacctct
    10921 cgggctcgga ctttcgggcc ggtgggctcg tgcgggtgcg tctgacctgc accgtctcct
    10981 acgccgatct gggcctggtc ggactgccgg gccagacgac cgtgacctcg gtgagcaccg
    11041 cccccctcga catcttccgg agggccgggt gacccgcgca gcccggcgac aaccggcctt
    11101 ctc
//
LOCUS       DAORPW010000074        10809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1374_length_10809_cov_712.035057, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000074 DAORPW010000000
VERSION     DAORPW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1374_length_10809_cov_712.035057"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLB86_07505"
     CDS             <1..482
                     /locus_tag="PLB86_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC65022.1"
                     /translation="QLRWLPASGWTPPAYDPGMFLQQLLLPALSLGLVQGAVLTRYVR
                     SAVLDVLREDFLRTARAKGLTPMRALVRHGLRNAAVPVVTVLGLQLATLLVGAVVIER
                     VFVIPGLGSLLLDAVGNRDLIVVQDVVMVLVVAVLIVNFVVDLLYLAIDPRLRTVAR"
     gene            479..1354
                     /locus_tag="PLB86_07510"
     CDS             479..1354
                     /locus_tag="PLB86_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC65023.1"
                     /translation="MSRLSRGAEAVMPPPGRRSRAGVEATLLVGGGIVAAITVLALVS
                     LVWTPYDVGEINATERLATSSARHWLGTDKFGRDVVSLLMVGSRTTLLVGLVSVGVAA
                     VIGVPLGILAAMVPKWWGEVLMRANDLLLAFPALLLAIMLTAVHGQGVVTAMIAIGIS
                     SIPSFARVVRSGAIGVMKTEYVVAARAAGRRPIAIALRHVLPNVGSLVIVQASVSFAI
                     AVLAEAALSYLGLGAPPGTPSWGRMLQEGQELLFVAPRLEVFPGVAIALAVLGFNLLG
                     DGLRDHFDPRLEDRR"
     gene            1393..2430
                     /locus_tag="PLB86_07515"
     CDS             1393..2430
                     /locus_tag="PLB86_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65024.1"
                     /translation="MPPTRTPADTPPPADVARVRDLAVTVGHKPLLIDVDLTVRRGER
                     VGLIGESGSGKSLTALAIMGLLPEDLAPTGSVVVTGQGPGGVARTTSVLEAGEHGLAA
                     MRGRDVAMVFQEPMTALNPTMRVGAQVAEVMTIHRTVADRGAAARRAVELLDKVGLPD
                     AAEAARAYPHQLSGGQRQRVVVAMALANDPALLICDEPTTALDVTVQALVLDLIVRGV
                     EARDAGLLFITHDLAVVASVCERVLVMYGGRVVEAGPVLDVFTRPRHRYTRGLIGASD
                     LDLSRPDGGLYTIQGSVPAAGRFPAGCVFRNRCTHATVECEAVPPWVGSGPEEGFACV
                     HPVVHHGGGGS"
     gene            2442..3218
                     /locus_tag="PLB86_07520"
                     /pseudo
     CDS             2442..3218
                     /locus_tag="PLB86_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            3380..4450
                     /locus_tag="PLB86_07525"
     CDS             3380..4450
                     /locus_tag="PLB86_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRC65025.1"
                     /translation="MTENPAIRPGRSNSLTDVDGIRVGHATRIGDGWLTGTTVVLAPD
                     GGAVGGVDVRGGGPGTRETDLLDPRNMVDRVHAVVLSGGSAFGLAAADGVMVRLASAG
                     VGFPVGGPGEVVPIVPAAVIFDLGRGGSFGKRPDAAMGNDALVVANDGPVQQGCVGAG
                     TGARAGGLKGGVGSASAVLPDGTTVAALIVVNAVGSCVDPLTGELYAARFGLPGEFPS
                     LGSVSEADLAGARERAAAAGPGLGLGPGLATTIGVIATDATLTKAQCAKVSGIGHDGM
                     ARAIRPVHTMFDGDTLFTLASGARGAAGAPDPLAFHALLDAAGDCVTRAIGHAMLSAT
                     TVTPPAGEIRCLRDAFPSAFAH"
     assembly_gap    4486..4585
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4802..5647
                     /locus_tag="PLB86_07530"
     CDS             4802..5647
                     /locus_tag="PLB86_07530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC65026.1"
                     /translation="MDNDAQPLTPTAPATSAPAPADLGALFRLDARRAVVIGAGSGIG
                     QAIALGIAAHGAEVVCVDRDLAAAQTTAHQAGTGPGWGAMTAYQLDVLDTQAVTRAVE
                     DLGAVDILVVTPAVNVRKRLLSYTGEEFDSVVRLNLRAAFDLMTGFGRGMAQRGRGSI
                     IGCSSIRSLTTEPGQGVYAMTKAGLVMLLRTLAAELGPQGVRVNAIAPGVIDTPLTAP
                     IKADEQWYAAYGAKSILGRWGRPHELAGAAVYLASDAASYVTGTVLFVDGGWTAIDGR
                     FTPPS"
     gene            5890..8076
                     /gene="malQ"
                     /locus_tag="PLB86_07535"
     CDS             5890..8076
                     /gene="malQ"
                     /locus_tag="PLB86_07535"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRC65027.1"
                     /translation="MPDPAHSVEAPTAPSLEQLQALAKAYGVGVDFWGFYGDLHAVSA
                     LTLTKVLAALGVDASTEQACERALADRAAQPWRQLLPPTVVMRENAPTLVRAHVHHGD
                     EVRVWVVDEAGAEWPVTQAEDNTSPHEVDGVTLGQASFWLPQELPLGWHLIRAESHGR
                     QAESTLIVVPNRLPTADALPAKAGRARTWGLMTQLYSVRSARSWGVGDVEDLADLAAI
                     SGAAGADWILVNPLHAAEPTPPMEASPYLPTTRRFFSPLYIRIEAIPESIYLTTAARR
                     HISLIWTQATARNHDASEVPRDAAYASKLEALQLVYAVPRSPSRDGRFAAFRAEHGRG
                     LEDFALWCAVRERSAEDAVIWEELRPHDEAAFALRQRLRDRIDFHCWLQWVLDEQLEA
                     AQASALRAGMALGIIHDLAVGVHPLGADAWVLRDVLAQNAEVGAPPDMYNQQGQNWSQ
                     PPWHPQRLAEAGYAPFRDMLRTILRHAGGIRVDHVLGLFRLWWVPLGLPADQGTYVYY
                     DHEALVGILCLEAQRAGAVVIGEDLGTFEPWVRDYLAGRGLLGTSILWFEYAADKAPL
                     APEAYRRGCLASVNTHDLPPTAGYLAGEHIALRDRLGLLTRSVEEEIAADREEQGRVL
                     DMCRRRGLLPEEGEAMPDGSEVGEQQIVEALYALLASAPSVMQGVALVDAVGERRVQN
                     QPGTDKEYSNWKIPLAGPEGAVVSVEELATNPRAQSLFRTVREALA"
     gene            complement(8126..8398)
                     /locus_tag="PLB86_07540"
     CDS             complement(8126..8398)
                     /locus_tag="PLB86_07540"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRC65028.1"
                     /translation="MSHWVLEYRYADPDARARVRPDHLAYMNGLHEDGRVVMGGPVGD
                     GGGALVVLRVDSEDEVHAIIAADPYTAAGVGADHVVRPWTVAIGGN"
     gene            complement(8473..9399)
                     /locus_tag="PLB86_07545"
     CDS             complement(8473..9399)
                     /locus_tag="PLB86_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65029.1"
                     /translation="MARRLEEVTRGDIDAIRYDDLSFDGSHGHVGVTLFMPSIRQLGI
                     KGEPEIISPYSAGWGEGLGAARASCEGLVRSANVMAATIEYPRKRVAVHEILQFRTEV
                     FGEVIRHIRERTMYDWATLVVAGYSRGTAPARMATVEHADVVDGVVLVAPTWFSQQVK
                     PRDLAARGLHESARGMAQAGWLDRLTLVGASVRLAQEMLAHPFELRNDIAAISQEGAA
                     DLQDVLDTGVRVGVVAGRQDELCQVDGIRTVVDRLPEPAAVMYREVDSDHFSYFLSPK
                     PLKVVAEVVTALGDFGTEAEAGDEAQAEGSGA"
     gene            complement(9510..>10809)
                     /locus_tag="PLB86_07550"
     CDS             complement(9510..>10809)
                     /locus_tag="PLB86_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRC65030.1"
                     /translation="ANARPVVFIGPYEHHSNELPWRETIADVVVIPQDLDGHISLSVL
                     RERLQEYAARPLKIGSFSAASNVTGIVSDSAAISTLLHEHGALSFWDFAAAGPYIDIE
                     MYSGGAGTTASNTAYKDAVFLSPHKFIGGPATPGVLVVRRELLTNRVPAMVGGGTVAF
                     VNPTEHRYLADPAHREEAGTPAIIESIRAGLVFQLKAAVGVDTIRAQEERLLHRAVSA
                     WTQEPTIQILGNLDAERLSIVSFVVKAPSGRYLHHNFVVAVLNDLFGIQSRGGCSCAG
                     PYGHALLGIDLDRSHEFEAEIAHGCEGIKPGWVRVNFNYFVSDEVTDYIVEAVRLIAR
                     DGWRLLPDYRFDNATGRWRHREGPVEPPLRLRDVGYAADGSFVFPRHTARAAEGELAA
                     YLIEGARILAERADDAGPQAEVSPGYDDLRWFELPAACMP"
BASE COUNT         1489 a         3691 c         3816 g         1713 t
ORIGIN      
        1 tccagctgcg gtggctgccg gcgagcgggt ggacgccacc ggcatacgac ccgggaatgt
       61 tcctccagca actgctcctg ccggccctct ccctcgggct cgtccagggc gcggtgctga
      121 cccgttatgt gcgttcggcg gtgctcgacg tgctccggga ggacttcctg cgcacggcgc
      181 gcgccaaggg actcaccccc atgcgggccc tcgtgcgcca cgggctgcgc aacgccgcag
      241 tcccggtcgt cacggtcctc gggctgcagt tggcgaccct gctggtcggc gcggtcgtca
      301 tcgagcgggt gttcgtcatt cctggcctcg ggtccttgct gctcgacgca gtcggcaacc
      361 gcgacctcat cgttgttcag gacgtcgtca tggtcctcgt cgtcgccgtc ctcatcgtca
      421 acttcgtcgt cgacctgctc tatctggcga tcgacccgcg cctgcggacg gtcgcccgat
      481 gagccgcctg tcgcgagggg ccgaagccgt gatgccaccg cccggtcggc gttcgcgtgc
      541 gggtgtcgag gcgacgttgc tcgtgggggg cgggatcgtc gcggcgatca ccgtcctcgc
      601 cctcgtgtcg ttggtgtgga cgccctacga cgtcggcgag atcaacgcca ccgagcggct
      661 cgcgacctcg tcggcgcggc actggttggg caccgacaag ttcggccgcg acgtcgtgtc
      721 actgctcatg gtcggttcgc gcaccacgct gctcgtcggt ctcgtctcgg tcggggtggc
      781 tgctgtcatc ggggttccgc tgggcatcct cgcggcgatg gtgcccaagt ggtggggcga
      841 ggtgctcatg cgggccaacg acctgctgct cgcgttcccc gccctgctgt tggcgatcat
      901 gctcacggcg gtccatggcc aaggcgtcgt caccgccatg atcgccatcg gcatctcgag
      961 catcccgtcg ttcgcccggg tcgtgcgcag cggggccatc ggggtgatga agaccgaata
     1021 cgtcgtcgcc gcgcgcgctg ccggtcgccg accgatcgcg atcgcgctgc ggcacgtgtt
     1081 gcccaacgtt ggcagcctcg tcatcgtcca ggcctcggtg tccttcgcga tcgccgtgct
     1141 cgccgaggca gccctgtcct atctcggcct cggcgcccct ccggggactc cctcatgggg
     1201 acggatgctg caggaagggc aggagttgct gttcgtcgcg ccccgcttgg aggtcttccc
     1261 gggggtcgca atcgccctcg cagtgctggg cttcaacctc cttggggacg ggctgcgcga
     1321 ccacttcgac ccgcgactgg aggaccggcg gtgaccgcgg caccggagca cacggcatac
     1381 ccagtggggg gtatgccgcc cacgcggact cccgctgaca cgccgccgcc ggcggacgtc
     1441 gcgcgcgtgc gcgacctcgc ggtgactgtc ggccacaagc cgctgctcat cgacgtcgac
     1501 ctcaccgtgc gccgtggcga gcgggtcggg ctcatcggcg agtcgggctc aggcaagtcg
     1561 ctcaccgcgc tcgcgatcat ggggttgctg cccgaggatc tggcgccgac ggggtcggtc
     1621 gtggtgaccg ggcaggggcc gggtggtgtg gcgcggacga cctcggtgct ggaggcgggg
     1681 gagcacgggc tcgcggcgat gcgtgggcgc gacgtcgcga tggtgtttca ggagccgatg
     1741 accgcgctca acccgacgat gcgggtgggg gcccaggtcg cggaggtgat gacgatccac
     1801 cggacggtgg ccgatcgcgg ggcggcggcg cggcgggcgg tcgagttgtt ggacaaggtg
     1861 ggcttgcccg atgcggcgga ggcggctcgc gcctatccgc atcaactctc cggaggtcag
     1921 cgccagcggg tcgtcgtggc gatggcgctg gccaacgacc cggcgctgct catctgcgac
     1981 gagccgacca cggcgttaga cgtcacggtg caggcgctcg tgctcgacct catcgtgcgt
     2041 ggggtggagg cgcgtgacgc gggactgctg ttcatcacgc acgacctggc cgtggtggcg
     2101 agcgtctgcg agcgggtcct tgtcatgtat ggcgggcggg tcgtcgaggc cggcccggtc
     2161 ctcgacgtct tcacgcggcc ccggcatcgg tatacgcgcg ggctcatcgg agcatctgat
     2221 ctggacctca gcaggcctga tggcgggctc tacacgatcc agggatcggt gccggcggcc
     2281 ggtcgcttcc ctgcggggtg cgtcttccgc aaccggtgca cgcatgccac ggtggagtgc
     2341 gaggcggtgc cgccgtgggt tgggtcaggg cccgaagaag ggttcgcctg cgttcatccg
     2401 gtcgttcacc acggaggtgg tggctcatga cggacgtggc gatctcggtg cgcaacgtgg
     2461 tgcgcgacta tcggcggccg cgaacgtcgc tgaccaagcc gggctcggtc gtgcatgccc
     2521 ttcgcggggt gagcttcgac gtggcgcgtg ggcagcggtt tggcatcgtg ggcgagtcgg
     2581 ggtgtggcaa gtccacgctg ttgcggatca tcgccggcct cgaccaaccg acctcggggt
     2641 cggtccaggt cgggggagtg gagatctccc ggctgcccga gcggcggctg aggttcttgc
     2701 gggcccagtt gcagctcgtc ttccaggacc ccatggggtc gctcgaccca cggatgcgcg
     2761 tccgggacat catcgccgaa ccccttgtgg cgcaagggca tcgggacaac attggccgcg
     2821 tgcacgagct gctggacgcc gtcgggctgc agcctgaggc ggcggatcgg ttcccgcatc
     2881 agttctcggg tggacagcgt caacggatct cgatcgcccg ggcgttggcc cctgacccac
     2941 gcatcctcgt cgccgacgag ccggtcagcg cgctcgacgt gtcggtgcgg gcccaggtgc
     3001 tcaacctgct cgatgagttg gtgcaacggc tctcgctcac cttggtgttc gtctcccacg
     3061 acctgtcagt ggtgcgacat gtctgcgaca cggtggccgt gatgagccga ggtgagatcg
     3121 tcgagatggg gccgatcggc gacgtgtatg acacgccggc ccatccgtat tcgcgccaac
     3181 tcgtcgccgc agctccccgg ctgcaggccg ctctggccgg ccgaaccgcg gcggtcctgg
     3241 cggcggaaac ccttgcggcc cgggccttgt cgcgcgacag cgtgtctcgc acggcctcgt
     3301 ctcgcaacgc cgctgactct gactcgaaac ccactccgcc gacgcccgag caaacgacac
     3361 ccccaccgaa ggacgcgcca tgaccgagaa cccggccatc cgcccagggc ggtccaactc
     3421 gctgaccgac gtggacggca tacgtgtcgg tcatgcgacc cgcatcggcg acggctggct
     3481 caccgggacg accgtggtgc tcgcgcccga cggcggggcg gtcgggggcg tcgacgtgcg
     3541 cggaggcggg cccggcaccc gggagaccga tctgctcgac ccgcgcaaca tggtcgatcg
     3601 ggtccatgcg gtcgtcctgt ccggggggtc ggcgttcggg ctggccgcgg ccgacggcgt
     3661 gatggttcga ttggcttctg cgggagtcgg attcccggtc ggagggcccg gcgaggtcgt
     3721 gccgatcgtg cccgctgcgg tgatcttcga cttggggcgc gggggatcgt ttggcaagcg
     3781 cccggacgcc gccatgggca acgacgcgct cgtcgtcgcc aacgacgggc cggtccaaca
     3841 agggtgcgtc ggggcgggca cgggcgcccg agccggaggt ctcaaaggcg gagtcgggtc
     3901 agcgtcggct gtcctgccgg acgggacgac ggtcgccgcg ctgatcgtcg tcaacgccgt
     3961 cggctcctgc gtcgacccgc tgactggcga gttgtatgcc gcccgcttcg ggcttcccgg
     4021 cgaattccct tctctggggt cggtgtccga ggctgatctg gctggggcgc gggagcgggc
     4081 tgccgctgcc gggccggggt tggggctcgg gcccgggctc gcgacgacga tcggggtcat
     4141 tgcgaccgac gccacgttga ccaaggcgca gtgcgcgaag gtctctggga tcggccacga
     4201 cgggatggct cgagcgatcc ggccggtgca cacgatgttc gacggtgaca cgttgttcac
     4261 gctggcatcc ggcgctcggg gggctgcggg tgctcccgac ccgttggcct tccacgcgct
     4321 gctggatgct gcgggggact gtgtcactcg cgccattggc cacgccatgc tgtcggccac
     4381 caccgtgacg ccgcccgctg gtgagatccg ttgcctgcgc gacgccttcc cctccgcctt
     4441 tgcccactga ccgctcgcgt caggcccgcc tccctcttcc cgaatnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnncctcc gcctttgccc actgaccgct cgcgtcaggc
     4621 ccgcctccct cttcccgaat ccctcacact cgccggtgat ctggacgaaa ccccggcccg
     4681 actcccccga atccctcaca cccgccgatg atctggtgag gcttgggggg tctggacccc
     4741 gaatccctca cactcgccgg cgatctctgg ggcccggagg ctggctgaca caatcgagcg
     4801 catggacaac gacgcccagc ccctcacacc gactgcgcct gccacatcgg cgcccgcccc
     4861 ggctgacctc ggtgccctct tccgcttgga cgctcgccgc gccgtcgtca tcggtgccgg
     4921 gtcaggcatc gggcaggcca ttgccctcgg aatcgcggcc catggcgccg aggtggtctg
     4981 cgtcgaccgc gacctggccg cagcgcagac gaccgcccac caagccggca cgggccctgg
     5041 gtggggggcg atgacggcat accaactcga cgtcttggac acgcaggcag tgactcgcgc
     5101 ggtcgaggac ctcggggccg tcgacatcct cgtcgtcacg ccggcagtta acgtccgcaa
     5161 acggctgctg agctacacgg gtgaggagtt cgactcggtc gtgcggctca acctgcgggc
     5221 ggccttcgac ctgatgaccg ggttcggacg cgggatggcg cagcgcggtc gcggcagcat
     5281 catcggctgc agctcgatcc ggagtctgac gaccgagccg ggccagggcg tctacgcgat
     5341 gaccaaggct ggtctcgtca tgctcctgcg caccctggct gccgagctcg gcccgcaggg
     5401 agttcgcgtc aacgccatcg ctcccggggt catcgacacg cccctcaccg ccccgatcaa
     5461 ggccgacgag cagtggtatg ccgcctacgg cgccaagagc atcctcggcc ggtgggggag
     5521 gccgcacgag ttggcaggcg ccgcagtcta tttggcctcg gacgcggcca gttacgtcac
     5581 ggggacggtc ctattcgtcg acggcggatg gacggcgatc gacggccggt tcacgccgcc
     5641 gagctgagtc ggatcgatcc cctttcgggc gtgagagcgc ccatgaagtc gtcgcaggtg
     5701 gcgtgatcac gcacgtagag gcggcttcat gggcggcttc acgcccggcc acgtcgcact
     5761 gagcggcacc cgagcagcag ccaagtcgca cccaagcagt cccgatccgg cggggcgaca
     5821 cggcataccc ctggggagtg gaagaatcgc cccggacgtt tcccccgcac ccccgacgag
     5881 gcaggaccga tgcccgaccc cgcccactcc gttgaggcgc ccacggcccc ctcgctggaa
     5941 cagttgcaag cactggcgaa ggcctatggc gtcggcgtcg acttctgggg gttctacggc
     6001 gacctccatg cggtctcggc gctgaccctc accaaggtcc tggctgccct tggcgtcgat
     6061 gcctcgaccg agcaggcctg cgagagagcc ctggccgatc gcgccgccca accctggcgc
     6121 caactgctcc ccccgacggt cgtcatgcgc gagaacgcgc cgaccctcgt ccgggcgcac
     6181 gtccaccacg gcgacgaggt gcgcgtctgg gtggtcgatg aggcaggcgc cgagtggccg
     6241 gtcacgcagg ccgaggacaa cacctcgccc cacgaggtcg acggggtgac cctggggcag
     6301 gcgagcttct ggctgcctca ggagcttccc ctcgggtggc acctcatccg cgccgaaagc
     6361 catggccgcc aggccgagtc gaccctcatc gtcgtcccca accggctgcc gaccgccgac
     6421 gcccttccgg ccaaggccgg gcgcgcccgc acctggggcc tgatgaccca gctctactcc
     6481 gtgcggtcgg cccgctcgtg gggcgtcggg gacgtcgagg atctggccga cctggccgcg
     6541 atcagcggtg cggccggcgc ggactggatc ctcgtcaacc ccctgcatgc ggctgagccc
     6601 acccctccga tggaggcctc gccctacctg ccgacgacgc ggcgcttctt cagcccgctc
     6661 tacatccgca tcgaggcgat ccccgagtcg atctacctca cgacggccgc ccgccgacac
     6721 atctcgctca tctggacgca ggcgaccgcc cgcaatcatg acgcatccga ggtgccgcgc
     6781 gacgcggcat acgcgagcaa gctcgaggcc ctccaactcg tgtatgccgt tccgcgcagt
     6841 cccagccgcg acggccggtt cgcggcgttc cgggccgaac acggccgggg cctggaggat
     6901 ttcgcgctgt ggtgcgccgt gcgcgagcgc agtgccgagg acgcggtcat ctgggaagag
     6961 ttgcgccccc acgacgaggc ggccttcgcc ctgcggcagc gcctgcggga ccggatcgac
     7021 ttccactgct ggctgcagtg ggtcctcgac gagcagttgg aggcggccca ggccagcgcg
     7081 ctgcgggcgg gaatggcgct ggggatcatc cacgacctcg ccgtgggcgt gcaccctctc
     7141 ggcgccgatg cctgggtgct gcgcgacgtc ctcgcccaga acgccgaagt cggcgcaccg
     7201 ccggacatgt acaaccagca gggccagaac tggtcacagc cgccgtggca tccgcagcgg
     7261 ctggccgagg cgggttacgc gcccttccgg gacatgctgc gcacgatcct gcggcacgcc
     7321 ggggggatcc gtgtcgacca tgtccttggg ctgttccggc tctggtgggt tccgctgggg
     7381 ctgccagccg accaggggac ctatgtctac tacgaccacg aggccctcgt cggcatcctc
     7441 tgcctggagg cgcaacgcgc cggcgccgtc gtcatcgggg aggacctggg caccttcgag
     7501 ccgtgggtgc gcgactacct cgcggggcgc ggcctgctcg gcacgtcgat cctgtggttc
     7561 gagtatgccg ccgacaaggc gccgttggcc cccgaggcct atcggcgcgg gtgcctcgca
     7621 tcggtcaaca cccatgactt gccgccgacc gccggttatc tcgccggcga gcacatcgcg
     7681 ctgcgcgacc gcctcgggct gctaacccgt tcggtcgagg aggagatcgc cgccgaccgc
     7741 gaggagcagg ggcgggtgct cgacatgtgt cgacgccggg gcctgctgcc ggaggagggc
     7801 gaggcgatgc ccgatggcag cgaggtcggc gaacagcaga tcgtcgaggc gctctatgcc
     7861 ctcctggcgt cggctccctc agtgatgcag ggggtcgctc tcgtcgacgc cgtgggcgag
     7921 cgccgggtgc agaaccagcc gggcaccgac aaggagtact ccaactggaa gatcccgctg
     7981 gccggtcctg aaggcgccgt cgtcagcgtc gaggagttgg cgaccaaccc gcgcgcccag
     8041 tcgttgttcc ggacggtccg cgaggcgctc gcctgaagtc ggccgtcagt cggcagccgg
     8101 acgcccgctg ctgcgagcga cgctgttagt tgccgccgat ggcgacggtc cacgggcgca
     8161 cgacgtgatc cgcgccgacc ccagcggccg tgtagggatc ggccgcgatg atggcgtgga
     8221 cctcgtcctc ggaatcgacg cgcagcacca ccagcgcgcc accgccgtcg ccgaccgggc
     8281 cgcccatgac gacgcggccg tcctcgtgca ggccgttcat gtaggccagg tgatccggcc
     8341 gcacccgggc gcgggcgtca ggatcggcgt aacggtattc caggacccaa tggctcatgg
     8401 cggcagcatg ccagaaccgg tgcgggccca cgcgcagccc tcaggctcgt gcgcgcccac
     8461 gtcgcgcagc cctcaggctc cggaaccctc ggcctgcgct tcgtccccgg cctccgcctc
     8521 ggtcccgaag tcgccgagcg ccgtgacgac ctcggcgacg accttgaggg gtttggggga
     8581 gaggaagtag gagaagtggt cggagtcgac ctcgcggtac atcaccgcgg ccggctcggg
     8641 gaggcgatcg acgacggtcc ggatcccgtc aacctggcag agctcgtcct ggcggccagc
     8701 gacgacgccg accctgacac ccgtgtcgag gacgtcctgc aggtcggccg ccccctcctg
     8761 ggagatggcg gcgatgtcgt tgcgcaactc gaacgggtgg gcgagcatct cttgggcgag
     8821 gcggacactg gcgccgacga gggtgagccg gtcgagccac ccggcctggg ccatcccgcg
     8881 ggcgctctcg tgcaacccgc gcgcggcgag gtcgcggggt ttgacctgct ggctgaacca
     8941 cgtgggggca acgaggacga cgccgtcaac gacgtcagcg tgctcgacgg tcgccattcg
     9001 cgccggggcc gtgccgcgcg agtagcccgc gacgacgagg gtggcccagt cgtacatcgt
     9061 ccgttcgcgg atgtggcgga tgacctcgcc gaagacctcg gtgcggaact gcaggatctc
     9121 gtggacggcg acccgtttgc gggggtattc gatcgtggcg gccatgacgt tggctgaccg
     9181 caccaggccc tcgcacgatg cccgggccgc gccgaggcct tcgccccagc cggccgagta
     9241 ggggctgatg atctcgggct cgcccttgat gccgagttgg cggatcgagg gcatgaacaa
     9301 ggtgacaccc acgtgcccgt gagagccgtc gaaactcagg tcgtcatacc ggatggcgtc
     9361 gatgtcgccc cgggtaactt cctctagtcg ccgtgccatt tgccaatggt gcccttcact
     9421 accgatgagt agcgtctttg gttgcgctga tcaccatcac gatttgggcc acagtatgcc
     9481 cacacctgag tatgaccacg cgtcgcggct cacggcatgc atgccgctgg caactcgaac
     9541 caacgcaggt cgtcataacc cggcgagacc tcggcctgtg gcccggcgtc gtccgcccgc
     9601 tcggcgagga tccgggcacc ctcgatcagg tatgccgcga gctcaccctc cgcggcccgc
     9661 gcggtgtggc gcggaaagac gaaactgccg tccgcggcat acccgacgtc tcgcagtcgc
     9721 agcggcggct cgaccgggcc ttcgcggtgc cgccaccgcc cggtggcgtt gtcgaagcga
     9781 tagtccggga gcaggcgcca cccgtcgcgg gcaatcagtc ggaccgcttc gacgatgtag
     9841 tcggtgacct cgtccgagac gaagtagttg aagttgactc gcacccagcc cggcttgatg
     9901 ccctcgcagc cgtgggcgat ctcggcctcg aactcgtgcg aacgatccag gtcgatcccg
     9961 agcagggcgt ggccgtaggg tccggcgcac gaacaaccgc cgcgggactg gatgccgaac
    10021 aggtcgttga ggacggcgac gacgaagttg tggtgcaggt agcggccgct cggagccttg
    10081 acgacgaacg agacgatcga gaggcgctcg gcgtcgaggt tgccgaggat ctggatggtc
    10141 ggctcctggg tccaggcgga cacggcgcgg tgcaagagcc gctcctcctg ggcgcggatc
    10201 gtgtcgaccc cgacggccgc cttgagttgg aacaccaggc cggcgcggat cgactcgatg
    10261 atcgctgggg tgcccgcctc ctcgcggtgg gcggggtcgg cgaggtaacg gtgctcggtc
    10321 gggttgacga aggcgaccgt gccgccgccg accatggcgg ggacccggtt ggtgagcaac
    10381 tcgcggcgca cgaccagcac gccaggggtg gcggggccgc cgatgaactt gtgcggcgac
    10441 aggaagaccg catccttgta tgccgtgttg ctcgccgtgg tcccggcgcc gccggagtac
    10501 atctcgatgt cgatgtaggg gccggccgcg gcgaagtccc agaaggacag cgcaccatgc
    10561 tcgtgcaaca gggtgctgat cgcggcgctg tccgagacga tgccggtgac gttggaagcg
    10621 gcggagaagg acccgatctt caggggccga gcggcatact cctgcagtcg ttctcgcagg
    10681 acggacaggg agatgtggcc gtcgaggtcc tgcgggataa cgacgacgtc ggcgatcgtc
    10741 tcgcgccacg gcaactcgtt ggagtggtgc tcgtagggcc cgatgaagac gaccggtctg
    10801 gcgttggcc
//
LOCUS       DAORPW010000075        10723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1396_length_10723_cov_897.106862, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000075 DAORPW010000000
VERSION     DAORPW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1396_length_10723_cov_897.106862"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..658)
                     /locus_tag="PLB86_07555"
     CDS             complement(101..658)
                     /locus_tag="PLB86_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65031.1"
                     /translation="MSRIPVSRRPGARLLVALATLMVVNGCASSTPGLVANSASYLAQ
                     GSGDGRITFGIQICAPPGVNVWVTDIQPLAWVGSGFDVLGFRLASYDAPGGITVATSG
                     FPPAIPTRPGEQVYAVGDHVAVQPCESAQRLSEVQVGLARRGPSGGGLSSVRITYGGT
                     VSGEIVTDVGMLFCGPSTTDCPVDR"
     gene            801..1994
                     /locus_tag="PLB86_07560"
     CDS             801..1994
                     /locus_tag="PLB86_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRC65032.1"
                     /translation="MTTAPALGPLAGVKIIEFAGIGPGPFAGMVLADLGADVIGVDRP
                     GGAGPFDLPVLRRGRRSIAVDLKSEAGREVILDLVASADALIEPYRPGVMERLGLGPD
                     AVLARNPRLVYARMTGWGQDGPQSPYAGHDLTYTATSGILSSFVRDPHDGSMPRPVLP
                     GPYLGDVAGGALLLVAGLLAALHSAAATGTGQVIDAAIMDGASYLSTFTHALRLGGMW
                     GGTPGNNILDTGAPFYEVYECADGRHLAVGALEPQFYDELLRLLAEHAGLDLGPDDPR
                     HRLNRLDQRRWPEAKALWEDFFRTRTRDEWCAFLERSDACVAPVLSLEEAPTHPHLGA
                     RAAYLPVGGDHGPELPVPAPRFSATPTQTRPSPARGAHTDELLAEIGYHPARIATLRS
                     TSVVA"
     gene            2083..3957
                     /locus_tag="PLB86_07565"
     CDS             2083..3957
                     /locus_tag="PLB86_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRC65033.1"
                     /translation="MSKPVQQLDRVVIRFAGDSGDGMQLTGDRFTSDAAFHGNDLSTL
                     PNFPAEIRAPQGTIHGVSSFQLHIADHDVLTPGDAPDVLVAMNPAALKANLADLPRGA
                     TIIVDTDEFSRRNLAKVGYAVSPLDDGSLESYHVHALPLTSITVDALAEHPLTRKDKE
                     RAKNMFALGLLSWLYTRPTSTTERFILSKFARKPDLLAANVAALRAGFAFGETTEDFA
                     HTYQVAAASMPDGTYRNITGNVALSYGLVAAAHQAGLRLVLGSYPITPASDILHTLSG
                     LKRFDVTTMQAEDEIAGVGIAIGASFGGALGVTTTSGPGLALKAEAIGLAVSLELPLV
                     VVNVQRGGPSTGLPTKTEQADLLQAMFGRNGESPVAIVAPQSPTDCFDAAIEACRIAT
                     AYRTPVILLSDGYLANGSEPWKVPVPADLPALDVTFTTEPNGVDASGLPVFQPYLRDP
                     ETLARPWAIPGTPGLEHRIGGIEKADVTGNISYDPDNHELMTKLRAGKIAKVADTIPG
                     LEVDDPDGDARVLVIGWGSTYGPIRQAARSARLTGHRVATTHLRHLNPFPLNTGEVLR
                     RYERVIMPEMNLGQCAMLLRAAYLVDVQSHTAVRGLPFKASELAEIIRTAVDGLEATK
                     "
     gene            3954..5033
                     /locus_tag="PLB86_07570"
     CDS             3954..5033
                     /locus_tag="PLB86_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRC65034.1"
                     /translation="MSIDLGMPSSGVAFVPQLAEGSPALGRKDYTSDQDVRWCPGCGD
                     YAILAAMQGFLPELGLRKENIVFISGIGCSSRFPYYLDTYGLHSIHGRAPTFATGLAT
                     AREDLSVWVITGDGDALSIGGNHLIHALRRNVNIKILLFNNEIYGLTKGQYSPTSRAG
                     TITKSSPLGSLDAPFTPVSVALGAEATFVARTMDSERKHLTEVLHAAARHRGAALVEI
                     YQNCPIFNDGAFDVLKDKDEAAARILWLKDGEEVATGDRVAVRADDGSIQVVPRAEAS
                     PTRIVRHSVDTEHPSSAFALSRLTSDGLLAHVPMGVFRDVSRPTYDDMVRGQVSAAVE
                     AAGGPATDADLAALLAGRDTWTVGG"
     gene            5030..5935
                     /gene="rarD"
                     /locus_tag="PLB86_07575"
     CDS             5030..5935
                     /gene="rarD"
                     /locus_tag="PLB86_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HRC65035.1"
                     /translation="MTPALNPGSEVRRGALFGTAAYMLWGFFPLYFHGLSPATPLEVL
                     AHRILWSLFFCLIILAWRRDVTWVRPLFARPRLLAGLTAAALLVAINWGVYVAAVLSG
                     HTRDAALGYFLNPLITVALGVVLLREPLRRLQWMAVAIGLVAAVYLTVAGGQVPVIAL
                     TLAVTFALYGLLKKRIGASLGALHGLTIETAVLLPIALLLLGPLSGLGGTHAGFGSVG
                     QAAGLILTGPITAIPLLLFAAAARRVPLVTIGLLQFLTPVMQLLCGLLLGEVVTPTQW
                     VGFVIVWVALGVLTVDSLRQVARSR"
     gene            5954..6424
                     /locus_tag="PLB86_07580"
     CDS             5954..6424
                     /locus_tag="PLB86_07580"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HRC65036.1"
                     /translation="MTITRLLRVLLVVFAAGLAVGVLMTHWLSLDVAQTYPEVAHLRA
                     PAFWAAVVGLVPLVVAAGAMWRFLALVDRGEAFSGASARLVRRVSVVFLVLAVYIAVA
                     AVVLGGALPQSSPTFTLAVMGAEAVALFLMAACALLGRLLREAGLPLHRNPLPA"
     gene            complement(6551..7549)
                     /locus_tag="PLB86_07585"
     CDS             complement(6551..7549)
                     /locus_tag="PLB86_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRC65037.1"
                     /translation="MSAPQVLSLPGVSPELAAALADGMGRVDALLRSTVDHDDPYVAA
                     ASAHLLAAGGKRFRPTLTLLAAQVCRGVTDEVIAAAVGVELTHLASLYHDDVMDEAAV
                     RRAVPSVNAAYDNTTAILVGDLLFGKASEIVAGLGAEAVRIQAQTFVRLCFGQIRDTR
                     ACPPDVDPIDYYLGVLADKTGALVATAARYGAMFGGGSPEMVEVLRVYGERLGMAFQL
                     ADDIIDIASDGAMSGKTPGTDLREGVDTLAVLFAKASADPADARLQELLAADLTDDVL
                     HAEALALLRANPALERARAHTREVGAQAREALAGLPDSDAKAALVALVDQVVERVG"
     gene            complement(7584..9173)
                     /gene="nuoN"
                     /locus_tag="PLB86_07590"
     CDS             complement(7584..9173)
                     /gene="nuoN"
                     /locus_tag="PLB86_07590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HRC65038.1"
                     /translation="MTTALSAAFTPATLDYVALLPVLIIFSAAMLGVLVEAFVPRTSR
                     YLAQVTITLVGIVAAMAALAFGAVTHRVSTAGIAGFNGTVKGSVVIDGPALFIQATIL
                     LFALLAALSLAERFGGVGADAFTPMGAATPGSPQEAQARKVGAMTAEVFPLFLFSVAG
                     MMLFPAAGDLLTMFVALEVLSLPLYLLCGLARRRRLLSQEASLKYFLLGAFSSAFFLF
                     GTALLYGFAGSVYLPDISAAITAQGSAFQGLLVPGVLLVLVGLLFKVGAVPFHMWTPD
                     VYQGAPTPITGFMAAGTKAAAFGAILRFVYVGVDRAQWDWRPAVVVVAALTMIIGAVL
                     SVTQTDMKRLLAYSSIAHAGFILVGVLSMERDGVSGVMFYIAAYGLTTIAAFAIVGLV
                     REGGSEATHLSQWTGLGKRHPWTAGVFAFLLLAFAGIPLTSGFTAKFAVFKPALESGT
                     AGTILVVLGLLCSAITVFVYVRIIVLMYFTDSTDDSVAVIEPSVFTTVTVATGTLFTL
                     VLGIAPASVLQLAERASQFIR"
     gene            complement(9170..10705)
                     /locus_tag="PLB86_07595"
     CDS             complement(9170..10705)
                     /locus_tag="PLB86_07595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRC65039.1"
                     /translation="MNQFPWLTVIGLIPLVGAVVLWLLPASLADRAKHIALGASLVTL
                     VFAVAAALQFSTSSTSKFQLVEEHSWIPQFGVSYALGVDGIALSLILMSVILVPVCLL
                     AAWNDLPAEEAHRERHYFALMLVLETFMVGVFAATDVFLFYVFFEAMLVPVYFLIGQF
                     GGPRRQYAAVKFLLFSLAGGLIMLAAVIGLYVQGPGGADGFLLSKLAGIQLAPGTEKW
                     LFLGFFIAFAVKAPMVPVHTWLPDAAAESRPATATLLVGVLDKVGTYGMIRFCLELFP
                     GASQWATPVVITLALVSILYGALLAIGQTDLMRLIAFTSISHFGFIVLGIFAMTSTSW
                     AGSSFYMINHGFSTAGLFLVAGMFISRRGSKRIPDFGGWQRVTPALAGVWLLVGLSGL
                     ALPGLGSFISEFLVLAGTFQRYQVVGVIATTSIILAALYILLMYQRTMTGPKPESAAG
                     TPDISYREKWVVGPLIALFLILGFYPKPVLDMVTPAVTTILTSIHVTDPAPAVGAAEG
                     STK"
BASE COUNT         1739 a         3743 c         3657 g         1584 t
ORIGIN      
        1 gcgggtggag ggacctgagg cgttggccgc gcaaagcggc caacgcctca gtgttccttc
       61 gtgatcttct cccgagcccg cggtcgatca gagatccctg ctaccggtcc accgggcagt
      121 cggttgtcga cgggccgcag aagagcatgc cgacgtcggt cacgatctct ccggagacag
      181 tgccgccgta tgtgattcgc acgctcgaca gaccgccgcc cgaaggaccg cgacgagcca
      241 gaccgacttg gacctcgctc agtcgctgcg cactctcgca cggctgcacc gcgacgtgat
      301 cgccgacggc atacacctgc tcccccgggc gcgtcgggat agcaggcggg aaccccgagg
      361 tcgccacagt gatacctccg ggcgcgtcgt aactggccaa ccggaagccg aggacgtcga
      421 agccgctgcc gacccacgcc agcggttgga tatcggtgac ccacacgttc acccccggcg
      481 gcgcacagat ctgaatcccg aaggtgatcc ggccgtctcc actgccctgg gcgaggtaac
      541 tggccgagtt tgccaccagc ccgggggttg acgatgcgca cccgttgacg accatcagcg
      601 tcgcgagcgc cacgaggagt cgcgcaccag gtctgcggct caccgggatc ctgctcatcg
      661 ccacacccct gacaccggac gaaacggatg aatgcgacgg tacgcgcccg accgccgcaa
      721 cggaaggcaa gcccgtggtg tgatccatca gcgctggagc cagacccgcg agtggcaggc
      781 ccacggcata ccgtggatgc gtgaccacag cgcctgccct tggcccgctc gcgggcgtga
      841 agatcatcga gttcgcgggg atcggccccg ggccgttcgc ggggatggtg ctcgccgatc
      901 tcggggccga cgtgatcggg gtcgaccggc ccggcggcgc gggccccttc gacctgccgg
      961 tgctgcgccg tggccggcgg tcaatcgccg tcgacctcaa gagtgaggca gggcgcgagg
     1021 tcatcctcga tctcgtcgcg agcgccgacg cactcatcga gccctaccga ccgggtgtca
     1081 tggagcggct cgggctcggc cccgacgccg tcctggcccg caacccgcgg ctggtctatg
     1141 cgcggatgac cggatggggg caggacggac cgcaatcccc gtatgccggt cacgacctca
     1201 cctacaccgc cacctcgggc atcctctcca gcttcgtgcg ggacccgcac gacgggagta
     1261 tgccgcggcc ggtcctgccg gggccctacc tgggcgacgt cgcgggtggg gcgctgctgc
     1321 tcgtcgccgg cctgttggcg gcgctgcact cggccgccgc aacgggcacc gggcaggtga
     1381 tcgacgcggc gatcatggac ggcgcgtcct atctgagcac cttcacccat gcgctgcgcc
     1441 tcggagggat gtggggtggc actccgggca acaacatcct cgacacgggc gccccgttct
     1501 acgaggtcta cgagtgcgcg gacgggcgac acctggccgt cggcgccctc gagccgcagt
     1561 tctacgacga gttgctgcgc ctgctcgccg agcatgccgg gctcgacctc gggcccgacg
     1621 acccgcgcca ccggctgaac cggctcgacc agaggcgttg gccggaggcg aaggccttgt
     1681 gggaggactt cttccgcacc cggacccggg acgagtggtg cgcatttctg gagcgctctg
     1741 acgcgtgcgt cgcgccggtg ctctcgctgg aggaagcgcc gacgcacccg cacctgggtg
     1801 cgcgggcggc atacctcccg gtgggtggcg accacgggcc cgagttgccg gtgcccgcgc
     1861 cgcgcttctc ggcgacgccg acccagaccc ggccatcccc ggcgaggggt gcccacaccg
     1921 acgaactcct ggccgaaatc ggctaccacc cggcgcggat cgcgacgctt cgcagcacgt
     1981 ccgtggtcgc ctgaacgcca attcctgatc aacgtcctac gatgaggaga ggcgccgtgg
     2041 cccacacggc gatcgggagt cctagggccg ggtgaccacc acatgagcaa gcctgtccag
     2101 cagttggatc gcgtcgtgat ccgattcgcc ggtgattccg gcgacgggat gcaattgacc
     2161 ggcgaccggt tcacctccga cgcggcgttc cacggcaatg acctgtcgac gttgcccaac
     2221 ttccccgcgg agatccgggc gccccagggc actatccacg gggtgtcgag cttccagctg
     2281 cacatcgccg accacgacgt gctcaccccc ggtgacgcgc ccgacgtgct cgtcgcgatg
     2341 aacccggcgg cgctcaaggc caacctcgcc gacctgccgc gcggcgcgac gatcatcgtc
     2401 gacaccgacg agttcagtcg gcgcaacctc gccaaggtgg ggtatgccgt gtcgccgctg
     2461 gacgacggca gcctggagag ctaccacgtc cacgccctgc cgctcacctc gatcaccgtc
     2521 gacgcgctcg ccgagcaccc gttgacccgc aaggacaagg aacgggcgaa gaacatgttc
     2581 gccctgggtc tgttgtcgtg gctctacacc cggccgacat cgacgaccga gcggttcatc
     2641 ctcagcaagt tcgccaggaa gcccgacctg ctggctgcca acgtggccgc cctgcgggcc
     2701 ggcttcgcct tcggcgagac gaccgaggac ttcgcccaca cctatcaggt ggccgcggcc
     2761 tcgatgcccg acggcaccta ccgcaacatc accggcaacg tcgcgctgtc ctacggtctc
     2821 gtcgcggccg cccaccaggc cggcctgcgt ctggtgctcg ggtcctaccc gatcaccccg
     2881 gcctcggaca tcctgcacac gctgtcgggt ctcaagcggt tcgacgtgac gacgatgcag
     2941 gccgaggacg agatcgccgg agtcgggatc gccatcggag cctccttcgg cggggcgctt
     3001 ggcgtgacga caacgtcggg tcctgggttg gccctcaagg ccgaggcgat cggcctggcc
     3061 gtctcgctcg agctccccct cgtggtcgtc aacgtccagc gcggcgggcc ctcgaccggc
     3121 cttcccacca agaccgagca ggccgacctc ctgcaggcga tgttcggccg caacggcgag
     3181 tcgccggtcg cgatcgtcgc gccgcagtcc ccgaccgact gcttcgacgc ggccatcgag
     3241 gcgtgccgga tcgccacggc ataccgcacg ccggtcatcc tgctgtccga tgggtacctc
     3301 gccaacgggt ccgagccgtg gaaggtcccc gtcccggccg atctgccggc cctcgacgtg
     3361 accttcacga ccgagcccaa cggggtcgat gccagcggtt tgccggtctt ccagccctat
     3421 ctgcgtgacc ccgagaccct cgcgcgcccg tgggcgatcc cggggacgcc tggcctggag
     3481 caccgcatcg gcggcatcga gaaggccgac gtcaccggca acatctccta cgaccccgac
     3541 aaccacgagc tgatgaccaa gttgcgcgcc ggcaagatcg ccaaggtcgc cgacaccatc
     3601 cccggcctgg aggtcgatga ccccgacggc gacgcccgcg tgctcgtcat cgggtggggc
     3661 tcgacctacg gtcccatccg ccaggcggcc cgctcggcgc gtctcaccgg gcaccgggtc
     3721 gccacgacgc acctgcgtca cctcaacccg ttcccgctca acaccggcga ggtgctgcgc
     3781 cgctacgagc gggtcatcat gcccgagatg aacctcgggc aatgcgcgat gctgctgcgc
     3841 gcggcatacc tcgtggacgt gcagtcgcac accgcggtcc gcgggctacc gttcaaagcc
     3901 tcggaactcg ccgagatcat ccgcacggcc gtcgacggac tggaggcgac gaagtgagca
     3961 tcgacttggg tatgccgtcc agcggggtcg cgttcgtccc gcaactggcc gaggggtcac
     4021 cggcgctggg gcgcaaggac tacacctccg accaagacgt ccggtggtgc ccgggctgcg
     4081 gcgactacgc gatcctcgcg gcgatgcagg gcttcttgcc tgagctcggg ctgcgcaagg
     4141 agaacatcgt cttcatctcc ggcatcgggt gctcctcgcg gttcccttac tacctggaca
     4201 cctatgggtt gcactcgatc cacggtcgtg cgccgacctt tgccaccggg ctggcgacgg
     4261 cgcgcgagga cctgtcggtg tgggtcatca ccggtgacgg cgacgcgctg tcgatcggcg
     4321 gcaaccacct catccacgcg ctgcgccgca acgtcaacat caagatcctc ttgttcaaca
     4381 acgagatcta cgggctgacc aaggggcagt actcccccac gtcgcgcgcg gggaccatca
     4441 cgaagtcctc cccgctgggc tcgctcgacg cgccgttcac gccggtctcc gtggccctcg
     4501 gggccgaggc gaccttcgtc gcgcggacca tggactccga gcgcaagcac ctcaccgagg
     4561 tcctgcatgc ggcggcccga caccgcggtg cggcgttggt cgagatctat cagaactgcc
     4621 cgatcttcaa cgacggggcc ttcgacgtcc tcaaggacaa ggacgaggcg gccgcccgga
     4681 tcctctggct caaggacggc gaggaggtcg ccaccggcga ccgcgtggcc gtgcgcgctg
     4741 acgacggctc gatccaggtg gtcccgcggg ccgaggccag cccgacgcgg atcgtccggc
     4801 acagcgtcga caccgagcac ccgtcgtcgg ccttcgcgct gtcccgcctt acctctgacg
     4861 ggctgctggc gcacgtgccc atgggtgtgt tccgggacgt gtcgcggccg acctacgacg
     4921 acatggtccg cggtcaggtc tcggctgcgg tcgaggccgc cgggggcccg gcgaccgacg
     4981 ccgatctcgc cgcgctgctc gccggtcggg acacctggac cgtcggcggg tgacccccgc
     5041 cctcaaccca ggctccgagg ttcgacgcgg cgcactcttc ggcaccgcgg catacatgct
     5101 gtgggggttc ttccccctct acttccatgg gctgtccccg gcgacgccgc ttgaggtgct
     5161 ggctcaccgg atcctgtgga gcctgttctt ctgcctgatc atcctggctt ggcgtcggga
     5221 cgtgacgtgg gtgcggccgc tgttcgcccg tccgcggttg ctcgcggggt tgaccgccgc
     5281 ggccctgctc gtcgcgatca actggggggt ctacgtcgct gcggtgttga gcggacacac
     5341 ccgggacgct gcgctcggct acttcctcaa cccactcatc accgtggccc tcggcgtcgt
     5401 gctgctgcgc gaacccctgc gccgcctgca atggatggcc gtcgcgatcg gcctggtggc
     5461 ggcggtctat ctcacggtgg cgggcggtca ggtgccggtc atcgctctca cgctggcggt
     5521 gacgttcgcg ctctatgggc tgctcaagaa gcgcatcggc gccagcctgg gggcgctgca
     5581 cgggctcacg atcgagaccg ccgtgctgct gccgatcgcg ttgctgctgc tcgggccgtt
     5641 gtcggggttg ggcggcaccc atgcggggtt cggctcggtc ggtcaagcgg ctgggctcat
     5701 cctcaccgga ccgatcacgg cgatcccgtt gctgctgttc gctgccgcgg ctcgacgggt
     5761 gcccctcgtg acgatcgggt tgttgcagtt cctcacgccg gtcatgcagt tgctgtgcgg
     5821 cctgttgctc ggtgaggtcg tcaccccgac ccagtgggtg ggcttcgtca tcgtctgggt
     5881 ggccctgggg gtgctgacgg tcgattcttt gcggcaggtt gcccgaagtc gctgaggatc
     5941 ggacaatcag gacatgacga tcacccggtt gctccgtgtc ctgctcgtcg tcttcgctgc
     6001 ggggctcgcc gtgggcgtcc tcatgacgca ctggctgagc ctggatgtgg cgcagaccta
     6061 ccccgaggtc gcccacctgc gggcgcctgc gttctgggct gccgtcgtcg gcctcgttcc
     6121 actcgttgtc gcggccgggg ccatgtggcg cttcctcgcg ctcgtggacc ggggtgaggc
     6181 gttctctggt gccagtgcgc ggctggtccg acgggtcagc gttgtcttcc tcgtgctggc
     6241 ggtctacatc gcggtggccg cggtcgtcct aggcggcgcg ttgccgcagt cgagtccgac
     6301 cttcacgctc gccgtcatgg gtgccgaagc cgtcgcgttg ttcctcatgg ctgcctgcgc
     6361 cctgttgggg aggctgctgc gcgaggccgg attgccgctc catcggaacc ccctgccggc
     6421 gtgacgacgc acattgggcg gggcaggcgg ggatgaagcc gtccgtgaag tcgccagagg
     6481 tggtgtgatc acgcatgctg tcactgcttc acggacggct tcacacccgc gttcgtgacg
     6541 ttgcgggggt tcagccgacg cgctcgacga cctggtcaac cagcgctacc agagcagcct
     6601 tcgcgtcgct gtcgggcagc cccgccaacg cctcgcgagc ctgcgccccg acctcgcggg
     6661 tgtgcgctcg agcccgctcc aacgccgggt tggcccgcag cagcgccagc gcttcggcat
     6721 gaagcacgtc gtcggtgagg tctgcggcca gcaactcctg caaccgcgca tcggccgggt
     6781 cagcagacgc cttcgcgaac agcacggcga gcgtgtcgac cccctcgcgc aggtcggtcc
     6841 ccggcgtctt gccactcatc gccccgtccg aggcgatgtc gatgatgtcg tcggccaact
     6901 ggaacgccat ccccagccgt tcgccgtaga cccgcaacac ctcgaccatc tccggtgacc
     6961 caccgccgaa catcgcgcca tatcgggcgg ccgtcgccac cagcgcaccc gtcttgtcgg
     7021 cgagaacgcc gaggtagtag tcgatcgggt cgacgtcggg cgggcaggcc cgggtgtcgc
     7081 ggatctgccc gaaacacaac cgcacgaagg tctgcgcctg gatgcggacg gcctcggccc
     7141 ccagcccggc gacgatctcc gacgccttcc cgaacagcag gtcaccgacg aggatggccg
     7201 tggtgttgtc gtatgccgcg ttgacgctcg ggacggcccg acgcaccgcc gcctcgtcca
     7261 tcacgtcgtc gtgatagagc gaggccaggt gggtgagctc gacgcccacg gcagccgcga
     7321 tgacctcatc ggtgacgccg cggcatacct gcgccgcaag gagcgtcaac gtgggacgga
     7381 accgcttccc gcctgcggcg agcaggtggg ccgaggctgc ggcgacgtag gggtcgtcgt
     7441 ggtccacggt cgaacgcagc agcgcgtcga cgcgccccat cccgtcggcc agggcagccg
     7501 ccaactccgg gctcaccccc ggcagggaca gcacctgggg tgcactcatc gagtcggctt
     7561 cctggtgtcg gtggcgaccg aggtcaccga atgaactgcg acgcccgctc cgcgagctgg
     7621 agcaccgagg ccggcgcgat gccgaggacc agcgtgaaca gcgtgcccgt cgcgaccgtc
     7681 acggtcgtga agaccgacgg ctcgatgacg gcaacagagt cgtcggtgga gtcggtgaag
     7741 tacatgagca cgatgatccg gacatagacg aacacggtga tcgcgctgca cagcagaccc
     7801 aacaccacga ggatcgtgcc cgccgtgccg ctttcgagcg ccggcttgaa gacggcgaac
     7861 ttggcggtga accccgacgt gagcgggatg cccgcgaacg ccaacagcag gaaggcgaac
     7921 acgcccgccg tccacgggtg acgcttgccc aggccggtcc actgcgagag gtgggtcgcc
     7981 tccgacccgc cctcgcggac cagacccacg atcgcgaacg ccgcgatggt cgtcagaccg
     8041 tatgccgcga tgtagaacat cacgccgctc accccgtcgc gctccatgga gaggacgccg
     8101 accaggatga agcccgcgtg cgcgatcgag gagtaggcca gcagtcgctt catgtcggtc
     8161 tgggtgaccg agaggaccgc accgatgatc atcgtcaggg cggcgacgac aacgaccgcg
     8221 ggacgccaat cccactgcgc ccggtcgacc ccgacataga cgaagcgcag gatcgcaccg
     8281 aacgcggccg ccttggtgcc ggcggccatg aagccggtga tcggcgtggg ggcaccctga
     8341 tagacgtcgg gggtccacat gtggaacggc accgcgccga ccttgaagag caggccgacg
     8401 agcacgagga gcacacccgg gaccagcagg ccctggaacg cggacccctg cgccgtgatc
     8461 gcggcggaga tgtcgggcag gtagaccgag ccggcgaaac cgtagagcag cgcggtcccg
     8521 aagaggaaga acgccgacga gaaggcgccg agcaggaagt acttgagcga ggcctcctgc
     8581 gacaggaggc gacgccgccg ggccaacccg cagagcaggt agagcggcag cgagaggacc
     8641 tcaagcgcga cgaacatcgt cagcaggtca cccgcggccg ggaagagcat catgccggcg
     8701 accgagaaga ggaacagcgg gaagacctcg gcggtcatcg cgccgacctt gcgggcctgc
     8761 gcctcctgcg gcgatcccgg cgtcgcggcc cccatggggg tgaacgcgtc ggcgccgacg
     8821 ccgccgaacc gctcggcgag gctgagcgcg gccagcaggg cgaacaacag gatggtggcc
     8881 tggatgaaca gggccggccc gtcgatgacg accgaaccct tgaccgtccc gttgaaccca
     8941 gcgatccctg cggtgcttac ccggtgggtg accgccccga aggccagcgc ggccatggcg
     9001 gccacaatgc cgaccagcgt gatggtcacc tgggcgaggt agcgcgacgt gcggggcacg
     9061 aacgcctcga cgaggacgcc gagcatcgcc gcactgaaga tgatgagaac cggaaggagg
     9121 gcgacgtagt cgagcgtcgc cggcgtgaag gccgcagaca gcgcggtggt cacttggtgc
     9181 tcccttcggc cgcgccgacg gcgggcgccg gatcggtgac gtggatcgag gtgaggatcg
     9241 tcgtgaccgc cggggtgacc atgtccagaa ccggcttcgg gtagaagccg aggatgagga
     9301 acaaggcgat gagcgggccg acgacccact tctcccgata gctgatgtca ggcgtcccgg
     9361 cggcactctc gggcttcggg ccggtcatcg tgcgctggta catgagcagg atgtagagcg
     9421 cggccaggat gatgctcgtc gtcgcgatga cgccgacgac ctggtagcgc tggaacgtcc
     9481 cggccagcac gaggaactcg gagatgaacg agccgaggcc cggcagcgcg agaccagaca
     9541 ggccgacgag cagccagaca ccggccagcg ccggcgtgac tcgctgccac ccaccgaagt
     9601 cggggatccg cttgctgccg cggcggctga tgaacatgcc cgcgaccagg aagaggcccg
     9661 ccgtcgagaa gccgtggttg atcatgtaga agctcgaccc ggcccaactc gtcgaggtca
     9721 tcgcgaagat gcccaggacg atgaagccga agtggctgat cgacgtgaac gcgatgagcc
     9781 gcatgaggtc ggtctgcccg atcgccaaca gcgcgccgta gaggatcgac accagggcga
     9841 gcgtgatgac cacgggcgtc gcccactggc tggcacccgg gaacagctcc aggcagaagc
     9901 ggatcatccc gtaggtgccg accttgtcca ggacaccgac gagcagcgtc gcggtggccg
     9961 gtcggctctc cgccgcggcg tcgggcagcc acgtgtgcac gggcaccatc ggggccttga
    10021 ccgcaaaggc gatgaagaac ccgaggaaca gccacttctc ggttccgggg gccagttgta
    10081 tgccggccag cttgctcagc aggaagccgt cggctccgcc ggggccctgg acatacagcc
    10141 cgatgaccgc ggccagcatg atgagcccgc cggccaggga gaagagcagg aacttgacgg
    10201 cggcatactg ccgacgcggc ccgccgaact gcccgatgag gaagtagacc gggacgagca
    10261 tcgcctcgaa gaagacgtag aacaagaaca cgtcggtcgc ggcgaacaca ccgaccatga
    10321 acgtctcgag gacgagcatg agggcgaagt agtggcgctc ccgatgcgct tcctcggccg
    10381 gcaggtcgtt ccaggcggcc agcaggcaca ccgggacgag gatcaccgac atgaggatga
    10441 gcgacagggc gattccgtcg acaccgaggg cgtagctgac cccgaactgc gggatccacg
    10501 agtgctcctc gacgagctgg aacttgctcg tgctcgacgt cgagaactgc aacgcagccg
    10561 cgacggcaaa gacgagggtc accaacgacg cgccgagcgc gatgtgcttg gcccggtcgg
    10621 ccagcgaggc aggcagcagc cacaggacca cggccccgac aagcgggatc aggccgatga
    10681 cggtcagcca ggggaactgg ttcatcactg caccacccag acg
//
LOCUS       DAORPW010000076        10664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1405_length_10664_cov_978.763785, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000076 DAORPW010000000
VERSION     DAORPW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10664
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1405_length_10664_cov_978.763785"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..704
                     /locus_tag="PLB86_07600"
     CDS             99..704
                     /locus_tag="PLB86_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65040.1"
                     /translation="MGRLARAYIVAAVAATVSLAGCSSTPEPTLPSFGPTPTATASSP
                     QTPAATASPTATATATAVGAMNFLEMPAGFTAEQRAVGLAYQEYWKLASRAYQTPREP
                     HPDLGKAITGEALVGLNGYVNKLVSTGRHLEGPAEFKVVTVSVSGATAKACGSMLDSS
                     HEIDDVTGESKEAIDPIIRLYSATFTQSGGVWRVATLKRGC"
     gene            797..1837
                     /locus_tag="PLB86_07605"
     CDS             797..1837
                     /locus_tag="PLB86_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65041.1"
                     /translation="MRLVRSALICALAVLGLTALPANAWANNGGGGEASGGGYGVAVR
                     FSGDGAPSGGGSTVVRMPASCWWESVKYDYGGFQQDNPSSWVAWYDDLTNPLASHSYA
                     SDGNYTWGPRSMYQAAAASPNANALDLYSINCRDNATYCSGILMSYAGTPQVYDPGSC
                     PVPVTFRFFAGAPPQPLVDPQDLAQVARDYMVIPDPVAERNPKLDLTGGGTLVSLPTW
                     FWVTNPDAVGAATGGTRTIRAQAGPVWAEVVARTSGLSINSPAGGTSCSPDRAVRVYA
                     PGADDSTGCTVAFDAASVRYADGYPVDLSTSWSATWTGSGGTGGALPGLSRTVTTNVP
                     VAESQALVYSYR"
     gene            2122..2535
                     /locus_tag="PLB86_07610"
     CDS             2122..2535
                     /locus_tag="PLB86_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65042.1"
                     /translation="MTFRDMPAGFTAEQRAVGLAYQEYWKLAWQAYRTPREPHPDLGK
                     AITGEALVGLNGYVNKLVSTGRHLEGPAEFKVVTVSVSGATAKACGSMLDSSHEIDDV
                     TGESKEAIDPIIRLYSATFTQSGGVWRVATLKRGC"
     gene            2628..3668
                     /locus_tag="PLB86_07615"
     CDS             2628..3668
                     /locus_tag="PLB86_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65043.1"
                     /translation="MRLVRSALICALAVLGLTALPANAWANNGGGGEASGGGYGVAVR
                     FSGDGAPSGGGSTVVRMPASCWWESVKYDYGGFQQDNPSSWVAWYDDLTNPLASHSYA
                     SDGNYTWGPRSMYQAAAASPNANALDLYSINCRDNATYCSGILMSYAGTPQVYDPGSC
                     PVPVTFRFFAGAPPQPLVDPQDLAQVARDYMVIPDPVAERNPKLDLTGGGTLVSLPTW
                     FWVTNPDAVGAATGGTRTIRAQAGPVWAEVVARTSGLSINSPAGGTSCSPDRAVRVYA
                     PGADDSTGCTVAFDAASVRYADGYPVDLSTSWSATWTGSGGTGGALPGLSRTVTTNVP
                     VAESQALVYSYR"
     gene            3725..4942
                     /locus_tag="PLB86_07620"
     CDS             3725..4942
                     /locus_tag="PLB86_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRC65044.1"
                     /translation="MTAPVLDLALARLAAIEDMLLDFRRDVHRHPELSFAEHRTTANV
                     ADALTDAGVKVTLLPGSGLVADLGAEHAAYRIALRADLDALALPERTHLPYASVVPGV
                     AHACGHDVHVTVCLGAALALVSVQEHLVSSGLGVRVIFQPAEEVIPGGALPICESEWL
                     DDVDEIYAVHCDPTLDVGSIGLRVGPLTAAADRVTVRLSGSGGHTSRPQLTQDLVFAL
                     GKVITEVPAALGRRVDPRSSASLVWGTVEAGHAANVIPESGSVSGTLRVLDAGAWDNA
                     GALVEELVSSAVAPYGVQMAIEHIRGVPPVVNAPEAVDLLTDAAKRVGVDPVPTEQSM
                     GGEDFAWYLTKVPGAMARLGTRMPGGPTFDLHQGDLVVDEASVTHGARVLTAAVLSRR
                     LPGGVAVGDVPSR"
     gene            5074..6120
                     /locus_tag="PLB86_07625"
     CDS             5074..6120
                     /locus_tag="PLB86_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRC65045.1"
                     /translation="MRHSIKAAALLTVAALGLAACSSSASTTTTTTAASTAGAPAAGA
                     LKVGMAFDTGGRGDGTFNDLAVKGLERAAKELGATAKELSPNADGTNRADLLRQLADG
                     GYSPIFGVGYNYGEPMDQVAKEYPKVQFVRIDGAPSTVANVAVMDFAEQEGSFLVGVA
                     AALKSKTHHVGFIGGNESVVINRFGAGFAQGAKAADPTIVVDDKRLAAADSGAGFGDV
                     PGAKVAAAAMYQNGADIVFTAAGGSFAGSFPAAKDAGKLVIGVDSDQFNTVSDAALKP
                     LILTSMIKRVDNAVFLSVQAFKTTGKLTSHSYNLKDEGVAYSTSGGFVDDIKSKLEDY
                     KAKIIAGTIVVNTK"
     gene            6217..7791
                     /locus_tag="PLB86_07630"
     CDS             6217..7791
                     /locus_tag="PLB86_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65046.1"
                     /translation="MPTEVHPTPPSAQSSPAAAIIELRGITKRFPGVVANRDIDLVVR
                     AGEVHALVGENGAGKSTLMKVLYGMYHPEEGQILVNGTPTPFRGPNDAIAAGVGMVHQ
                     HFMLADNFTVLENIILGSEPQSRGRLDIDAARSRIDQISRDYGLDLDPDALVENLTVA
                     DRQRVEIAKVLFRGARVLILDEPTAVLVPQEVDELFEHLQEMTAEGLAIVFISHKLDE
                     VLKVADVITVIRRGTTVASVDPGSVTARQLAELMVGSELPKPDTRESTVTDHEQLHVT
                     GLALAAPSGKPILDDISFVVHRGEVAGFAGVEGNGQSELVAALLGLVKPDSGTISVDG
                     RDISNETTMGRRNAGIAVVPEDRHRQALLLASPLWENRLLGHTHDPRLFTSWGWLKKG
                     ATVSDTVRIMADYDVRAPGPETLVGSLSGGNQQKLIIGRELESDPSILIAAHPTRGVD
                     VGAQAAIWDRIRAARAKGLATVLISADLEELIGLSDVLYVIYKGRLVAKLDPADVTPQ
                     ILGTYMTGAANKEASA"
     gene            7788..8969
                     /locus_tag="PLB86_07635"
     CDS             7788..8969
                     /locus_tag="PLB86_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC65047.1"
                     /translation="MTRLRRVVLTGVLPATLALLVAVLVLAVVLLVLGVDPVKSLVAL
                     FDFGETPQAQANQMRAWVEGVVPLFLSGLAVSLGFRMGLFNIGVEGQYRIAALVAAWF
                     AATVPLPGALAIILTIIVAMLAGALYALIPAVLKVTRGVNEVITTIMLNSIVGGLIGY
                     LLRGPFRDPKLGETGTGRTADIRTDAWFPGFEDPFGWLGMLTPSRPLGGFIVVALIVG
                     VAVALGLNRTRFGFNLRASGSNPEAAATSGVQSSRMIVQAMLLSGGIAGLVGLPQILG
                     DVHAYGADSFFLGFGFSGIAVALLGRNNPLGIGLAAILFAFLNRSGPSLQNVEVPPSV
                     VSIAQGVIVLSVVIVNEVLRRRLLRMEDRKAAAQTPATPAAPGTSVSGTPGPATAGAS
                     A"
     gene            8966..10231
                     /locus_tag="PLB86_07640"
     CDS             8966..10231
                     /locus_tag="PLB86_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC65048.1"
                     /translation="MSTSTASAPTATTVARRARRNRLLLLAGLGLVALSITRVISGEG
                     VLTASGTINAMVWLTLPVALAGLGGLISERAGVVNIGLEGMMMLGVWGAGFAGWRWGP
                     LAALVAALIGGALGGLLHALATVTFGVDHIVSGVAINLLAAGTVRFLSELTFIPSTGG
                     GPTQSPPMSGNIPTFDIPVLASGPDVLGSVERTGIPVLADAAGVLRGLMVGITPYELL
                     GLAMFPLTAYLLWRTALGLRLRSAGENPWAAESLGVNVVRVKYLAVVWGGALAGLGGL
                     FLVMFTGSYREGMTNGRGYIGLAAMIFGNWRPGGLAAGATLFGYADAVRLRSQNSLAV
                     LALFIFAGIVVIALGVLWLRRGRRLPGAIFIGIGVTFLLGYLGLDSLPNEVTTLTPYI
                     VTLVVLSVSSQNLRMPKADGMVYRKGEGH"
     gene            10279..>10664
                     /locus_tag="PLB86_07645"
     CDS             10279..>10664
                     /locus_tag="PLB86_07645"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRC65049.1"
                     /translation="MPSPDWVALQRAAIEVRDTAYAPYSGFPVGVAGLVDDGRVVVGC
                     NVENASYGVGLCAECGMVSALVAGGGGRLVAVSCVGPEGDTLMPCGRCRQLLWEHGGP
                     DCLVATSTGPRPMSELLPDAFGPANLG"
BASE COUNT         1617 a         3695 c         3516 g         1836 t
ORIGIN      
        1 ggcccgcaaa gatgaccgtg cgagcgctat ctccgggggg tcggttaggg tcgtgggcgt
       61 tggtcaggtg gcgcgtcaag gtaagacagg ggaaccccat gggacggctt gctcgggcat
      121 acatcgtcgc tgcagtggca gcgaccgtct cgcttgctgg gtgcagttca acgccggagc
      181 cgacgttgcc gagcttcggt cccaccccca cggcaacagc gtcgtcaccc cagactccgg
      241 cggcgactgc cagcccgacg gcgaccgcca ccgcgacagc agtcggcgcg atgaacttcc
      301 ttgagatgcc agccggcttc accgctgagc aacgagccgt cgggctggcg tatcaggagt
      361 attggaagct cgcctcgcgg gcgtaccaaa ccccgcgtga accgcacccc gacctcggca
      421 aggccatcac cggcgaggcc ctcgtcgggc tcaacggcta cgtcaacaaa ctggtgagca
      481 ccggacgcca ccttgagggg cccgcggagt tcaaggtcgt cacggtctcg gtttcaggag
      541 cgactgccaa ggcatgcggc tcgatgctcg actcttcgca tgagatcgat gacgtcacag
      601 gtgagtcgaa ggaagcgatc gatcccatca tccgcctcta ctcggcaacg ttcacccagt
      661 cgggcggtgt gtggcgggtc gctacgctca agcgaggctg ctgatcgcct cgctggcgcc
      721 caatcgcgac gacggtaccg tcgggtaaag tgccgagtcg tgaggtcccg actcacaggt
      781 cgacagggga acgcgcatga gactggtcag atcggcactc atctgtgccc tggcagtgtt
      841 gggcctgacc gcgctgcccg cgaatgcttg ggcaaacaat gggggtggag gggaagcctc
      901 gggcggcggc tacggcgtcg cggtccgctt ctccggggat ggcgccccga gcggcggggg
      961 atccacggtc gtgcggatgc ccgcatcgtg ctggtgggag tcagtgaagt acgactacgg
     1021 aggattccag caggacaatc cctcgtcgtg ggttgcctgg tacgacgatc tcaccaatcc
     1081 gctggccagt cattcgtacg cgtccgacgg caactacacg tggggccctc gctcgatgta
     1141 ccaggcggct gcggccagtc ccaacgcgaa cgccttggac ctgtacagca tcaactgtcg
     1201 ggacaatgca acttactgtt ccggcatcct catgtcgtat gccggaactc cgcaggtcta
     1261 cgacccgggg agttgcccgg tcccggtcac cttccgcttc ttcgctggtg cccctcctca
     1321 gccgctggtc gacccgcagg atctcgccca ggttgctcgg gactacatgg tcatccccga
     1381 tcccgttgct gaacgcaacc cgaaactgga cctcaccggc ggcgggaccc tggtctccct
     1441 gccgacgtgg ttctgggtca ccaaccccga cgctgtcggg gcggccaccg gcgggacgcg
     1501 gacgatccgg gctcaggccg ggccggtgtg ggccgaggtc gtcgcgcgca ccagcgggct
     1561 gagcatcaat tcgccggcgg gtgggacgag ttgctcgccg gatcgtgcgg tccgtgtcta
     1621 tgccccaggg gccgacgact cgacgggctg cacggtcgcg ttcgatgcgg cgtcggtccg
     1681 gtatgccgat gggtatcccg tcgatctatc gacgtcgtgg tcggcgacgt ggaccgggtc
     1741 cggtggcact ggaggtgcct tgcccggcct ctcgcggacc gtcacgacca acgtccccgt
     1801 tgccgagtcg caggccctgg tctactccta ccgatgaccg tgcgagcgct atctccgggg
     1861 tgggtggcgt ggggctggtc cgttagggtc tggggcgttg gtcaggtcgg cgcgtcaagg
     1921 caggggagca ccatggcacg gcttgcacga gcacatatcg ctgcagtggc agccgtcgtc
     1981 gccctcgctg ggtgcacctc aacgccggag ccgaccttgc cgagtttcgg tgccacgccc
     2041 tcggcaacag cgtcgtcacc tcagactccg gcggcgacgg ctaacccgac ggcgaccgcc
     2101 accgcgacag cagccgacgc catgacgttc cgcgacatgc cagccggctt caccgccgag
     2161 caacgagccg tggggctggc gtatcaggag tattggaagc ttgcctggca ggcgtaccgg
     2221 acccctcgtg aaccgcaccc cgacctcggc aaggccatca ccggcgaggc cctcgtcggg
     2281 ctcaacggct acgtcaacaa actggtgagc accggacgcc accttgaggg gcccgcggag
     2341 ttcaaggtcg tcacggtctc ggtttcagga gcgactgcca aggcatgcgg ctcgatgctc
     2401 gactcttcgc atgagatcga tgacgtcaca ggtgagtcga aggaagcgat cgatcccatc
     2461 atccgcctct actcggcaac gttcacccag tcgggcggtg tgtggcgggt cgctacgctc
     2521 aagcgaggct gctgatcgcc tcgctggcgc ccaatcgcga cgacggtacc gtcgggtaaa
     2581 gtgccgagtc gtgaggtccc gactcacagg tcgacagggg aacgcgcatg agactggtca
     2641 gatcggcact catctgtgcc ctggcagtgt tgggcctgac cgcgctgccc gcgaatgctt
     2701 gggcaaacaa tgggggtgga ggggaagcct cgggcggcgg ctacggcgtc gcggtccgct
     2761 tctccgggga tggcgccccg agcggcgggg gatccacggt cgtgcggatg cccgcatcgt
     2821 gctggtggga gtcagtgaag tacgactacg gaggattcca gcaggacaat ccctcgtcgt
     2881 gggttgcctg gtacgacgat ctcaccaatc cgctggccag tcattcgtac gcgtccgacg
     2941 gcaactacac gtggggccct cgctcgatgt accaggcggc tgcggccagt cccaacgcga
     3001 acgccttgga cctgtacagc atcaactgtc gggacaatgc aacttactgt tccggcatcc
     3061 tcatgtcgta tgccggaact ccgcaggtct acgacccggg gagttgcccg gtcccggtca
     3121 ccttccgctt cttcgctggt gcccctcctc agccgctggt cgacccgcag gatctcgccc
     3181 aggttgctcg ggactacatg gtcatccccg atcccgttgc tgaacgcaac ccgaaactgg
     3241 acctcaccgg cggcgggacc ctggtctccc tgccgacgtg gttctgggtc accaaccccg
     3301 acgctgtcgg ggcggccacc ggcgggacgc ggacgatccg ggctcaggcc gggccggtgt
     3361 gggccgaggt cgtcgcgcgc accagcgggc tgagcatcaa ttcgccggcg ggtgggacga
     3421 gttgctcgcc ggatcgtgcg gtccgtgtct atgccccagg ggccgacgac tcgacgggct
     3481 gcacggtcgc gttcgatgcg gcgtcggtcc ggtatgccga tgggtatccc gtcgatctat
     3541 cgacgtcgtg gtcggcgacg tggaccgggt ccggtggcac tggaggtgcc ttgcccggcc
     3601 tctcgcggac cgtcacgacc aacgtccccg ttgccgagtc gcaggcgttg gtctactcct
     3661 accgatgacc gtgtctgctg accgtgtcta gtgcgcgctc gcgcgctcac tagactgccc
     3721 cctcgtgacc gccccggtgc tcgacctcgc gcttgcgcgc ctcgccgcga tcgaggacat
     3781 gctccttgac ttccggcgcg acgtccatcg ccaccctgag ttgtccttcg ccgaacaccg
     3841 cacgaccgcg aacgtggccg atgcgctgac cgacgcgggt gtcaaggtca ccttgctgcc
     3901 cgggtcgggg ctggtcgccg acctgggagc cgagcacgcg gcataccgga tcgccttgcg
     3961 ggccgacctc gatgcgttgg cgctgccgga gcggacgcac ctgccctacg cctcggtggt
     4021 gcccggggtg gcccacgcgt gcggtcacga cgtccacgtc acggtctgcc tgggcgcggc
     4081 tctggccctc gtctcggtcc aggaacactt ggtcagcagc gggctgggcg tccgggtgat
     4141 cttccaaccc gccgaggaag tcatccccgg cggcgcgctg ccgatctgcg agtcggaatg
     4201 gctcgacgat gtcgacgaga tctatgccgt ccactgtgac cccaccctcg atgtagggag
     4261 catcggcctg cgcgtcggcc cgctgacggc tgcggccgac cgggtgaccg ttcggctctc
     4321 cggctccggt ggtcacacgt cgcggcccca actcactcag gacctggtct tcgcgctcgg
     4381 caaggtcatc accgaagtcc ctgccgcgct cggccgacgg gttgacccgc gctcgtccgc
     4441 gagcctggtc tgggggacgg tcgaagccgg gcacgcggcc aacgtcatcc ccgagagcgg
     4501 cagcgtgtcg gggaccctcc gggtgctgga cgcgggggcg tgggacaacg ccggtgccct
     4561 ggtcgaggaa ctcgtctcat ccgcggtcgc gccgtacggc gtgcagatgg ccatcgaaca
     4621 catccgcggc gtcccgccgg tcgtcaatgc ccccgaagcg gtagacctgc tcacggatgc
     4681 ggccaagcgc gtcggcgtcg acccggtccc gacggagcag tcgatggggg gcgaggactt
     4741 cgcctggtac ctcaccaagg tgcccggtgc gatggcccga ctgggcacgc gtatgccggg
     4801 tggtccgacc ttcgacctgc accaagggga ccttgtggtg gacgaggcca gcgtgaccca
     4861 tggggcgcgt gtgctcactg cggcggtgct gtcgcgacgg cttccgggag gcgtcgccgt
     4921 gggcgacgtt cccagtcggt aacgaacgtt ccgagtcggt aacaaacccg cacggtgtag
     4981 ggcagtcctc gccggtgcct gtaggcgtgg cgctacgctc gcgatatccg cgcgccggca
     5041 accccggcgc cttcggtcat ggaggagata cctttgcgtc actcaatcaa ggctgcagcg
     5101 ctgctcactg tcgctgcgct cggactcgca gcctgcagca gcagcgcgag caccacgaca
     5161 accacgacag ccgcctcgac ggcaggggcc cctgcggcgg gcgccctcaa ggtcggcatg
     5221 gccttcgaca ccggtgggcg cggcgacggc accttcaacg acctcgcggt caagggtctt
     5281 gagcgcgcag ccaaggagct tggtgccacc gccaaggaac tgagccccaa cgccgacggc
     5341 accaaccggg cagacctgtt gcgccagctg gccgatggcg gctacagccc gatcttcggc
     5401 gttggctaca actacggcga gcccatggac caggtggcca aggaataccc gaaggtccag
     5461 ttcgtccgca tcgacggagc cccgtcaacg gtggcgaacg tggcggtcat ggacttcgct
     5521 gagcaggagg gttccttcct cgtcggtgtg gctgcggccc tgaagtccaa gacgcaccac
     5581 gtcggcttca tcggcggcaa cgaatccgtc gtcatcaacc gcttcggtgc gggcttcgcg
     5641 cagggcgcca aggccgccga ccccacgatc gtcgtcgatg acaagcgtct cgccgcggcc
     5701 gactcgggtg ccggtttcgg cgacgtcccg ggagccaagg tcgctgctgc ggccatgtac
     5761 cagaacggtg ccgacatcgt cttcaccgcc gctggcggct cgttcgcagg gtcgttccct
     5821 gcggccaagg acgccggcaa actcgttatc ggcgtcgaca gcgaccagtt caacaccgtg
     5881 tccgacgcgg cgctgaagcc gctcatcctc acctcgatga tcaagcgcgt cgacaacgcc
     5941 gtcttcctca gcgtccaggc cttcaagacc accgggaagc tcacgtcgca cagctacaac
     6001 ctcaaggacg aaggggtcgc ctactcgacc tctggtggct tcgtcgacga catcaagtcc
     6061 aagctcgagg actacaaggc caagatcatc gcgggcacga tcgtcgtcaa caccaagtag
     6121 ttcgctgtcg atcggacaag gacgtccgcc tgacacgtac ccacgtgggg ggcccggcac
     6181 ctgccgggcc ccccacggct atggaaggat gcgcgcatgc ccaccgaggt gcacccgacg
     6241 cccccgtctg cccagtcctc gccggccgca gcgatcatcg agctgcgagg catcaccaag
     6301 cgatttcccg gcgtcgtcgc gaaccgggac atcgacctgg tcgtgcgcgc cggagaggtc
     6361 cacgcgctcg tcggggagaa cggcgccggt aagtcgacgc tgatgaaggt cctttacggg
     6421 atgtaccacc cggaggaagg gcagatcctc gtcaacggca ctccgacacc gttccgcggg
     6481 cccaacgacg ccattgccgc aggggtcggc atggtgcatc aacacttcat gctggccgac
     6541 aacttcaccg tcctggagaa catcatcctg ggcagcgaac cccaatcccg ggggcggctg
     6601 gacatcgacg ctgcccgcag caggattgac cagatctcgc gggactacgg cctggatctt
     6661 gacccggacg cactcgtcga gaatctcacc gtggccgacc ggcaacgcgt cgaaatcgcc
     6721 aaggtgcttt tccgcggtgc gcgagtcctc atcctcgacg agcccaccgc cgtcctcgtg
     6781 ccgcaagagg tcgacgagct cttcgagcat cttcaggaga tgacggctga gggcctggcg
     6841 atcgtcttca tctcgcacaa actcgacgag gtgctcaagg tggccgacgt catcactgtc
     6901 atccggcgtg ggacgacggt cgccagcgtc gatcccgggt cagtgacggc ccgtcagctc
     6961 gccgagctca tggtcggctc cgaactgccc aaaccggata cccgcgagtc caccgtgacc
     7021 gaccacgagc aactgcacgt caccgggctc gcgcttgccg cgccgtcggg taagccgatc
     7081 ctcgacgaca tctccttcgt cgtccatcgc ggagaggtcg ccgggtttgc cggtgtcgag
     7141 gggaacggcc agagcgagct cgtcgccgcc ctactcgggc tcgtcaagcc cgacagcgga
     7201 acgatctcgg tcgacggtcg cgacatcagt aacgagacga cgatggggcg acgcaacgcc
     7261 ggcatcgcgg tcgttcccga ggaccgccac cgtcaggcgc tccttctcgc ctcgccgctg
     7321 tgggagaacc ggctcctcgg gcacacgcac gacccgcgct tgttcacctc gtggggctgg
     7381 ctcaagaagg gcgccaccgt ctctgacacg gtcaggatca tggcggacta cgacgtgcgg
     7441 gcacccgggc cggagactct cgtcggatcg ctctccggcg gcaaccagca gaaactcatc
     7501 atcggtcgcg agcttgagtc cgacccgtcc atcctcatcg cagcccaccc cacccgcggc
     7561 gtcgacgtcg gggcccaggc ggcgatctgg gatcgcatca gggcggcgcg agccaaaggc
     7621 ctggccacgg ttctcatcag cgccgacctt gaggagctca tcggcctgtc cgacgtcctc
     7681 tacgtcatct acaagggaag gctcgtcgcc aagctagacc cggctgacgt gacaccccag
     7741 atcctcggca cctatatgac cggggctgcg aacaaggagg cctcggcatg acccgcttgc
     7801 gccgggtagt gctcaccgga gtcctccccg ccaccctggc gttgctcgtg gcggtcctcg
     7861 tgctggccgt cgtgctcctg gtgctcggcg tcgacccagt gaagtcgttg gtggccctgt
     7921 tcgacttcgg cgagactccg caggctcagg ccaaccagat gcgcgcttgg gtggagggcg
     7981 tcgtcccgct cttcctctcg ggcctcgcgg tgagcctcgg cttccgcatg gggctgttca
     8041 acatcggtgt ggaagggcag taccgcattg ctgcgctggt ggcggcgtgg ttcgctgcga
     8101 ccgtcccact ccccggcgct ctcgccatca tcctgacgat catcgtcgcc atgctcgccg
     8161 gcgcgctcta tgccctgatc cctgcggtgc tcaaggtgac gcggggagtc aacgaggtca
     8221 tcacgacgat catgctcaac agcatcgtcg gaggcctcat cggctatctg ctgcgcggcc
     8281 cgttccgcga cccgaagctc ggcgaaacgg ggaccggacg aaccgccgac atccggaccg
     8341 acgcctggtt ccccggtttc gaggacccgt tcgggtggct cggcatgctg accccgagcc
     8401 gaccgctcgg cggcttcatc gtcgtggcgc tcatcgtggg cgtcgcagtg gccctcggcc
     8461 tgaaccgcac ccgcttcgga ttcaacctgc gggcgtctgg aagcaacccc gaggcggccg
     8521 ccacgagcgg cgtccagtcg tcccggatga tcgtgcaggc catgctcctg tccggtggca
     8581 tagccgggct ggtgggactg ccccagatcc tcggcgatgt ccacgcctac ggtgccgaca
     8641 gcttcttcct cgggttcggc ttctccggta tcgccgtggc gctcctcggc cgcaacaacc
     8701 ctctgggcat cggcttggcg gccattcttt tcgcctttct caaccggtcc gggccttccc
     8761 ttcagaatgt cgaggtcccc ccatcggtgg tgtcgatcgc ccagggcgtc atcgtcctgt
     8821 cggtcgtcat cgtcaacgag gtccttcgtc gcaggttgct gcgcatggag gaccgcaagg
     8881 ctgccgcgca gacccccgca actcccgcgg cccccggcac ctcggtgagc ggcacccccg
     8941 gaccggcaac cgcaggagcg tcggcatgag cacctccacc gcatcagcgc ccactgccac
     9001 gactgtcgcc cggcgcgctc gacgcaaccg cctgctgctc ctcgccggcc tcggattggt
     9061 cgccctctcg atcactcggg tcatctcggg tgagggcgtc ctcaccgcgt cgggcaccat
     9121 caatgcgatg gtgtggctca cccttcccgt cgccctggcc ggcctcggcg gcctgatcag
     9181 cgagcgggcc ggcgtcgtca acatcggcct cgagggcatg atgatgctcg gtgtctgggg
     9241 ggccggcttc gccgggtggc gctggggacc actggccgcg ctcgtggccg ctctcatcgg
     9301 tggtgccctc ggcggcctgc tgcatgccct tgcgaccgtc accttcggtg tcgaccacat
     9361 cgtcagcggt gtcgccatca accttcttgc cgcaggcacg gttcgcttcc tctccgagct
     9421 caccttcatc ccaagcacgg gcggtgggcc gacccagtcg ccccccatgt ccggcaacat
     9481 tccgaccttc gacatccccg tcctcgcatc cgggcccgac gtgctgggca gtgtggaacg
     9541 gaccggcata cccgtgctcg ccgatgccgc gggcgtcctg cgcgggctga tggtcggcat
     9601 caccccctac gagctcctcg gcctggcgat gttcccgctc acggcatacc tgctctggcg
     9661 gaccgcgctg ggtctgcggc tgcgctccgc aggtgagaac ccgtgggctg cggagtccct
     9721 cggtgtcaac gtcgtccggg tcaagtacct cgccgttgtc tggggcggcg cgctcgcggg
     9781 cctcggtggc ctcttcctcg tcatgttcac gggcagttac cgcgagggca tgaccaacgg
     9841 tcgcggttac atcggcctcg cggcgatgat cttcggcaac tggcggcccg gcgggctcgc
     9901 cgcaggcgcc accctcttcg ggtatgccga tgccgtccgc cttcgttcgc agaactccct
     9961 tgcggtgctc gcgctgttca tcttcgccgg catcgtcgtc atcgcgttgg gtgtcctgtg
    10021 gttgcgccgc ggacgccgac tgcccggtgc gatcttcatc ggcatcggcg tgaccttcct
    10081 gctgggctac ctcggcctgg attcccttcc gaacgaggtc accaccctga ccccgtacat
    10141 cgtcaccttg gtcgtcctat cggtctcctc gcagaacctg cgcatgccca aggccgacgg
    10201 catggtctat cgcaagggcg aaggccattg acccagccgc ccccgcctga ccgcgcggac
    10261 gcggcatacc cctcgggtat gccgtcgccg gactgggtcg cgctgcaacg agcggccatc
    10321 gaggtgcgcg acacggcata cgcgccgtat tcggggttcc cggtcggcgt ggcgggcctg
    10381 gtcgacgacg gccgggttgt cgtgggctgc aatgtggaga acgcgagcta cggcgtgggg
    10441 ctgtgcgccg agtgcgggat ggtgtcggcc ctggtggctg gcggcggcgg tcggttggtg
    10501 gccgtgtcgt gcgtcgggcc cgaaggcgac acgctcatgc cgtgcggccg ctgccgacaa
    10561 ctgctctggg aacacggcgg gcccgactgc ctcgtcgcga cgtcgaccgg gccgaggccg
    10621 atgtccgaat tgcttcccga cgcgttcggt cccgccaacc tcgg
//
LOCUS       DAORPW010000077        10623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1415_length_10623_cov_689.815481, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000077 DAORPW010000000
VERSION     DAORPW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1415_length_10623_cov_689.815481"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1227)
                     /locus_tag="PLB86_07650"
     CDS             complement(100..1227)
                     /locus_tag="PLB86_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65050.1"
                     /translation="MKKSTLPTRLLALGAVFATAATLAVGSAGSAQAAKPGARGDVSS
                     ATASISAEAKAAGVAKHGAKATDAQALEAYWTPERMRAAKPAEEDSTLEASATAYVQK
                     QVADQKAGRKPVTNDGPVRSVEGASSKVFGPAATAGGVAAAYNPNLPYYVPTAYTAGK
                     VFFSKPDGNYVCSGTIINSEGKDTVWTAGHCVHGGSGGTWHTNWTFVPAYDDDLANPR
                     PYGTWSANQLWSRTAWTNSSDFSQDMGVAIMNTNWGWHIVDYFGGQGLTTNRGKSGVW
                     ENAFGYPAEWPFDGGNLMQCWGSTSPEWDAWFFWSQTVKIPCDMTRGSSGGGWLYGYN
                     GDWGQLNGVNSRIDRISGPTIMLSPYFDDDAWSLFNSTRWL"
     gene            complement(1361..1933)
                     /locus_tag="PLB86_07655"
     CDS             complement(1361..1933)
                     /locus_tag="PLB86_07655"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRC65051.1"
                     /translation="MSAGDVPVTGGPASAPADDAAYEDSEQGYAAGLRAWDGLPGIPA
                     SSGALIRDSRGRILVLKPTYKSGWTIPGGVMEANGETPWEACQREVFEETGLRVSAGR
                     LAAVDTRPAKARRAMGLRFLFDCGVVTDEQAASITLQSTELSDHAFLAPSEALARLRP
                     AVSRRVAAVLETGGCRYLEDGRPVAGVPDE"
     gene            1998..>2494
                     /locus_tag="PLB86_07660"
     CDS             1998..>2494
                     /locus_tag="PLB86_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRC65052.1"
                     /translation="MTTPRFAAALNAQIGNEFAAHLQYVAIAVHFDALTMPQMASFFY
                     VHAREERDHAMMMVRYLLDTEAEVTIPGSEAPITRFDGVVEPIELSLSQERTVTEQVY
                     ALTRIAREENDYGAEQFMQWFIKEQVEEVAKMSSLLTVAKRNTDSIADLEAYVAGQQA
                     NGGGV"
     assembly_gap    2495..2594
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2609..3262)
                     /locus_tag="PLB86_07665"
     CDS             complement(2609..3262)
                     /locus_tag="PLB86_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC65053.1"
                     /translation="MTVRVVVIDDQTLVRQGIVGLLRLSGEVEVIGQASDGAEGLEVV
                     AALRPDVVLLDLRMPILDGLGVLQALAAAGDRTPVLVLTTFPDDEALIAAMRAGARGY
                     LLKDVSLDQLVDAITTLAGGGTIIQPSVTEALVRQLAGTSADVDPVGLAPEPLTERET
                     EVLRLLSGGYSNREIAAAMFLAEGTIKNHVSAVLLKLGVRDRTRAVLRGLELGLIQR"
     gene            complement(3259..4440)
                     /locus_tag="PLB86_07670"
     CDS             complement(3259..4440)
                     /locus_tag="PLB86_07670"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC65054.1"
                     /translation="MQERHLEPLGIATAGMAWLSIGVVELIGLSDRASNWWWLAYLGY
                     GAAYLWCVVVAPKGHWPRRERAIALGVVTPLGVAVVALSSSSGFTSISLVMTAILLAN
                     LVSVRVVVGIVLVQTLAVVALLWTTFSDGGMAYAVSTGVAFFAFQLFALLMVVSVRAT
                     EAARNEADRARGEAEAANAALRAAQTQLATTSRTQERLRIARDLHDVVGHQLTALSVN
                     LQVASRTATGPVKDDLEVCRAVASDLLRDVREVVSQLRETHSAGPEFAEELRTIGHSV
                     PSPVVDVDVADDLPPLGAAEADVVRRCIQEIVTNAVRHSGATSVWIAVEELDGVVSVT
                     ARDDGQGSGGDGADVVMGHGLTGMVERFEALGGSVAWTARPGQGFTLQASVPVGRVAA
                     T"
     gene            4503..>4906
                     /locus_tag="PLB86_07675"
     CDS             4503..>4906
                     /locus_tag="PLB86_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC65055.1"
                     /translation="MTTGTDAGVCRPTTVDDMTSTPASGSTTTPAVIRASGLTKRYGD
                     LVAVDALDLVIAPGDIVAILGPNGAGKSTTIEMLLGLRTPTAGEVRVFGDAPNAKATR
                     ARVGAMLQESSAPESLTVAEAVDLVRHYYPFAL"
     assembly_gap    4907..5006
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5007..5411
                     /locus_tag="PLB86_07680"
     CDS             <5007..5411
                     /locus_tag="PLB86_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65056.1"
                     /translation="AEADALADRVILINRGRVIADGTPEQIKATVATRTVRLVTDVGV
                     AALAAFPGVRGVEVAPGESVGATHPTLVVQTATPEDLLARLFAGGHLVSQLTVTDTDL
                     ETAFIHLVGATTQTAPTATPDTTPEHVMESAS"
     gene            5408..6298
                     /locus_tag="PLB86_07685"
     CDS             5408..6298
                     /locus_tag="PLB86_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC65057.1"
                     /translation="MSTAGRTAYPADSSTTDSGDRTPRRLSPEASRMPGALGTLAAQL
                     RAELLSSSRVPEYVVGVVAVPIILFTMFGLPNAGVKLPGGTDVGAMMFASMSAYGVVS
                     LAIFTFGVGVADERGKGWLRRMRATPMPFWAYFVAKVVGSLAFCGLILLGTWAVAALG
                     GKVPFDGVRLAQTVLILLAGTVAFSTMGFSLAYWFRPKAASAIGNLVFLPLSFLSGFF
                     FPLDQLPDVLAKVAPYLPTYHFGQLVWSAIAPAQDVIAFGAHPEGSAWVHVGWVLASF
                     VGFGLLARAGYRRELSRAAQ"
     gene            6376..6936
                     /locus_tag="PLB86_07690"
     CDS             6376..6936
                     /locus_tag="PLB86_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC65058.1"
                     /translation="MTETPAAAAPVGDDLSLSHALLIDAFGRVAEGVPALLDGLGGEE
                     ILWRPDEDSNSIGWLVWHLARVQDDHLAGVAGREQIWTTAGFAKRFDLPYAARSIGYG
                     QSSQEVAAFSVADGSLLAAYHAAVHAETVEIVQGLTAADFARIVDRRWDPPVTAAVRL
                     VSVVNDITQHAGQAAYLRGLVERRRS"
     gene            7017..8312
                     /locus_tag="PLB86_07695"
     CDS             7017..8312
                     /locus_tag="PLB86_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRC65059.1"
                     /translation="MLTAFAVLLCLSALLAWANERWVHLPSTVAVTLAGAASSIVLIL
                     LDTQGWTFGLKQQAIELWQALDFTAFVLNGILSLMLFAGAMSLDATLMMRLRAGILTF
                     AGVSTAISTVLIGFGAWGVFHLVGLDIPLIWSLLFGALLSPTDPVAVLDMLKRAKVPK
                     KIETLIAGESLFNDGVGVVFFIVIASAAGVGGGHTEPSLTGALVVFVQEALGGIAFGA
                     LIGWITYRLCRSIEAASVEILLTLASVVGGYVLAAGLGLSGPLAMVVAGLVMSAGKER
                     AFGEHTRERIEGFWETLDEVLNILLFAFIGLDVLLTPTAVGEVLAGLLMIVVALVARF
                     VSVALPMTLVRRRDGYGPWTIRVLTWGGLRGGIAISLALGLPASPYRSALVTATYVVV
                     LFTIAVQGLTVMPIVRRAAAAEAAAEAAAGETENRAARP"
     gene            8420..8941
                     /locus_tag="PLB86_07700"
     CDS             8420..8941
                     /locus_tag="PLB86_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65060.1"
                     /translation="MLTVFIAIGAAGLALLVISLVVGEIHGGLFEWIDNDLFSGAAVS
                     AFLSALGFVGALVESTSGNTTVATLAGTGAGVGMAYAARGLTRSLQSGEGDTTVRSGD
                     LVGHRGHVVEAIPRDGMGMVSVSVAGHLTRLNARCEQPLSAGTGIEVTSVLSPTAVTV
                     RPIVVTPQDRALD"
     gene            8972..10450
                     /locus_tag="PLB86_07705"
     CDS             8972..10450
                     /locus_tag="PLB86_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRC65061.1"
                     /translation="MSANEFPVILVAIVGLIILLVVVGVLVASRYKVAGPNEAFIVTG
                     RKGKEIRDVETGEVSTDLSGQKVVMGGGIFVVPFVQRLHVLDLSSRRIMIQIRGAVSG
                     QGVKLNLDGVALVKVGGNEDSIRAAAQRFLTQQREIETFTQETLAGSLRSIVGSLTVE
                     QIIRDRAAFAQRVADESETSLTGQGLVLDTFQIQDITDDGSYLSDLGRPEAARVGQTA
                     AIAEANARQAAEQARLAAEQEIAIANRELALKQAEIKAQTDAATAQAAAAGPLAQADR
                     DQAILAEQEKVAVRKAALTERQLDTEVRRPADAARYRVEQEADARKAASIAAAQADAE
                     KARLTGEGEKARRTAEADALRAEGTAQAAAILATGEAEAQAMNKRADAYARYNEAAVL
                     QMLVEVLPKVARELASPMAAIDNLTVISTDGAGALPKQVTANLVQTMSMLKSATGFDL
                     EDIVKRYSGFVPAPATSVTAATTAPAAPAAETAPPATPATSA"
BASE COUNT         1553 a         3743 c         3489 g         1638 t
ORIGIN      
        1 ggtgagccag cggcatacca agaaggctag gcgagccgac cgaaacgcgc actggcgcgc
       61 ccggggattc cgggcgcgcc aggcgagggc taagaacggt cacagccaac gggtggagtt
      121 gaacaggctc cacgcatcgt cgtcgaagta gggcgagagc atgatcgtcg ggccggagat
      181 ccggtcgatc cggctgttga cgccgttgag ctggccccag tcaccgttgt agccgtagag
      241 ccagccgcca ccggacgagc cgcgggtcat gtcacacggg atcttcaccg tctgcgacca
      301 gaagaaccag gcgtcccact cgggtgaggt cgagccccag cactgcatga ggttgccgcc
      361 gtcgaaaggc cactcggccg ggtagccgaa cgcgttctcc cagacaccgc tcttgccgcg
      421 gttggtcgtc aggccctggc cgccgaagta gtcgacgatg tgccagcccc agttggtgtt
      481 catgatcgcg acgcccatgt cctgggagaa gtcagagctg ttcgtccacg cggtcctcga
      541 ccacaactgg ttcgccgacc aggtgccgta cggccgcggg ttggccaggt cgtcgtcgta
      601 ggccggcacg aaggtccagt tggtgtgcca ggtgccaccg ctgccgccgt gcacgcagtg
      661 accggcagtc cagacggtgt ccttgccctc ggagttgatg atggttccgg agcacacgta
      721 gttgccgtcg ggcttgctga agaacacctt gcccgcggtg taggcggtcg gcacgtagta
      781 gggcaggttc gggttgtagg ccgccgcgac gccaccagcc gtcgccgccg ggccgaagac
      841 cttgctcgac gcaccttcaa cactgcggac cggaccgtcg ttggtcaccg gcttgcggcc
      901 ggccttctgg tcggcgacct gcttctgcac gtatgccgtg gcgctggctt ccagcgtgga
      961 atcctcttcg gcgggcttgg cggctcgcat ccgctcgggc gtccaatacg cctcaagggc
     1021 ctgcgcgtcg gtggccttcg cgccgtgctt ggcgacgccg gcggccttgg cctcggcgga
     1081 gatcgacgcc gtggccgacg agacgtcgcc ccgggcccca ggcttggccg cctgtgccga
     1141 cccggcacta ccgacggcga gcgtggccgc ggtggcgaag acggcaccga gggcgagcaa
     1201 tcgagttggg agagtggact ttttcatgat catccttcgg ttggacccag gcaccccggc
     1261 actgggtttc aaccgtaaga gagcccggcg cccccgccct ggatacgcct tcaggtcagg
     1321 atcttccgaa ccccacaccg aaggcggagg tcagccctcg tcactcgtcg gggacgccgg
     1381 cgaccgggcg gccgtcctcg aggtagcggc agccgccggt ctccagcacc gcagcaaccc
     1441 gacggctcac ggccggccgc agtcgggcca gcgcctcact cggagcgagg aaggcgtggt
     1501 cgctgagctc ggtcgactgc agcgtgatcg aggccgcctg ctcgtcggtg accaccccgc
     1561 agtcgaagag gaaccgcagc cccatggccc tgcgcgcctt ggccggtcgg gtgtcgacgg
     1621 cggcgagccg cccggcgctc acccgcagcc ccgtctcctc aaagacctca cgctggcagg
     1681 cctcccacgg cgtctcgccg ttggcctcca tgacgccccc ggggatcgtc cacccgctct
     1741 tgtaggtcgg tttgagcacg aggatccgcc cgcggctgtc gcggatgagc gccccggacg
     1801 aggccgggat ccccgggagg ccgtcccacg cgcgcaaccc cgcggcatac ccctgctcgc
     1861 tgtcctcgta tgccgcgtcg tcggccggcg cgctcgccgg gccaccggtc acggggacat
     1921 ctcctgcgct catgcgccca ctctaggcag gcgggcgagg gggatgccga acggcggtgc
     1981 gccgctacag tggcccggtg accacgcctc gcttcgcggc cgccctcaac gcccagatcg
     2041 gcaacgagtt cgccgcccac ctgcagtacg tcgcaatcgc cgtgcacttc gacgcgctga
     2101 cgatgcccca gatggcgtcg ttcttctacg tgcatgcccg cgaggagcgc gaccacgcga
     2161 tgatgatggt ccgctacctg ctcgacaccg aggccgaggt gacgatcccc ggtagcgagg
     2221 cccccatcac gcgcttcgac ggggtggtgg agcccatcga gttgtccctg agccaggagc
     2281 gcacggtcac cgagcaggtc tacgcgctga cgcggatcgc ccgcgaggag aacgactacg
     2341 gcgcagagca gttcatgcag tggttcatca aggagcaggt cgaggaggtg gcgaagatgt
     2401 cgtcgctgct gacggtggcc aagcgcaaca ccgactccat cgccgacctg gaggcctatg
     2461 tcgccgggca gcaggccaac gggggcggcg tcgannnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnctccgc gaacccggtc accgctggat gagcccgagt tcgaggccac
     2641 gcaggacggc tcgggtccgg tcacggacgc ccaacttgag caggaccgcc gacacgtggt
     2701 tcttgatggt gccctcggcg aggaacatcg ccgccgcgat ctcccggttg gagtaacccc
     2761 ccgacagcag ccgcagcacc tcagtctcac gctcagtgag cggctccggc gccagaccga
     2821 ccgggtccac gtcagccgac gttcccgcga gttgacgcac gagcgcctcg gtgaccgacg
     2881 gctggatgat tgtgcccccg ccggcgagcg tcgtgatggc gtcgacgagt tgatcgaggc
     2941 tgacgtcctt gagcaggtat ccgcgagcgc cggcgcgcat cgccgcgatc agggcctcgt
     3001 cgtcggggaa ggtcgtcagg acgaggaccg gggtgcgatc acccgcggcg gcgagggcct
     3061 gcaggacgcc caggccgtcg aggatcggca tccgtaggtc gagcagcacg acgtccgggc
     3121 gcagcgccgc gacgacctcc aggccttcgg caccatcact ggcctgaccg atcacctcga
     3181 cctcgccgga gagtcggagc agcccgacga tcccctggcg gacgagggtc tgatcatcga
     3241 tgacgacgac gcggaccgtc atgtcgcagc cacccggccc accggcaccg acgcctggag
     3301 ggtgaaccct tggccgggac gcgcggtcca cgccaccgag cctcccagtg cctcgaagcg
     3361 ttccaccatc ccggtcaggc cgtgccccat gaccacgtcc gctccgtcac ccccggaccc
     3421 ttggccgtca tctcgcgcgg tcacgctgac cactccgtcg agctcctcga cagcgatcca
     3481 caccgaggtg gcgccactgt ggcgcacggc gttggtgacg atctcctgga tgcagcgccg
     3541 cacgacgtcg gcctcggcgg cacccagcgg cggcaggtcg tcagcaacgt cgacatccac
     3601 gaccgggctc ggcaccgagt gcccgatcgt gcgcaactct tccgcgaact ccggcccggc
     3661 actgtgggtt tcgcgcagct gactgaccac ctcgcgaacg tcgcgcagca ggtcggaggc
     3721 gacggcgcgg catacctcga ggtcgtcctt gaccggcccg gtggcggtcc gggaggcgac
     3781 ctggaggttg accgacaggg ccgtgagttg gtggccaacg acgtcgtgca ggtcgcgggc
     3841 gatccgcagc cgctcctgcg tgcgactggt cgtcgccaac tgcgtctgcg cggcccgcag
     3901 cgccgcgttg gccgcctcgg cctccccgcg agcccggtcg gcctcgttcc gcgcggcctc
     3961 ggtggcccgc accgacacga ccatgagcag cgcgaagagt tggaaggcga agaacgcgac
     4021 cccggtcgac acggcatacg ccatcccgcc gtcgctgaac gtggtccaca acagagcgac
     4081 cacggccagc gtctggacga ggacgatgcc gaccacgacg cgcaccgaca ccaggttggc
     4141 caacaggatc gccgtcatca ccaacgagat cgaggtgaac ccgctcgagg aggagagcgc
     4201 gacgacggca actcccagcg gggtcaccac gccgagtgcg atggcccgct cccgcctggg
     4261 ccaatgaccc ttcggcgcca ccacgacaca ccagaggtat gccgcgccgt aaccgaggta
     4321 ggccagccac caccagttcg acgcccggtc cgagagcccg atgagctcga cgacgccgat
     4381 cgacagccag gccatcccgg cggtggcgat ccccaacggt tcgaggtggc gttcctgcat
     4441 gacgtcgagc gtagggcggg cgaccccgtc ggcacccgtg ccggtcgtca tgggtgccgg
     4501 tcatgacgac cggcactgac gctggggtat gccgcccaac taccgtcgac gacatgacct
     4561 ccacacccgc ctccggctca acgaccacgc ctgccgtgat ccgtgccagc ggcctcacca
     4621 aacgctacgg cgacctcgtg gccgtcgatg ccctcgactt ggtcatcgcg cccggcgaca
     4681 tcgtggcgat cctcggcccc aacggcgcag gcaagtcgac gaccatcgag atgctgctcg
     4741 gcctgcgcac ccccacggcc ggggaggtcc gtgtcttcgg cgacgccccc aacgcgaagg
     4801 ccactcgggc tcgggtcggc gcgatgctcc aagagagcag cgcccccgaa agcctcaccg
     4861 tggccgaggc ggtcgatctg gtgcggcact actacccgtt cgccctnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnngccg aggccgatgc cctcgccgac cgggtgatcc
     5041 tcatcaaccg cggccgagtc atcgccgacg gcacgcccga gcagatcaag gccacggtcg
     5101 cgacgcgaac cgtccgactc gtcaccgacg tcggcgtcgc ggcgttggcc gccttccccg
     5161 gcgtccgcgg ggtcgaggtc gctccgggcg agtcggtggg tgccacgcac ccgaccctcg
     5221 tcgtgcagac cgccacgcct gaggacctgc tggctcgact cttcgccggt ggccacctgg
     5281 tgtcgcagtt gaccgtcacc gacaccgacc tcgagacggc cttcatccac ctcgtcggtg
     5341 ccaccaccca gaccgcaccg accgccaccc ccgacaccac ccccgagcac gtcatggagt
     5401 ccgcatcatg agcaccgccg gccgcacggc ataccccgcc gactcgtcga ccacggacag
     5461 cggtgaccgc accccccgac ggctgagccc cgaagcatcc cgtatgccgg gtgccctcgg
     5521 cacgttggcc gcgcagctgc gcgccgaact cctttccagc tcaagggttc ccgagtacgt
     5581 cgtcggggtg gtcgccgtgc cgatcatcct cttcacaatg ttcgggctgc ccaacgccgg
     5641 ggtgaaactc cccggcggga ccgatgtcgg ggcgatgatg ttcgcctcga tgtctgccta
     5701 tggcgtcgtc agcctggcga tcttcacctt cggggtgggc gttgcggacg agcgcggcaa
     5761 gggctggctg cggcggatgc gcgcgacgcc gatgccgttc tgggcctatt tcgtcgccaa
     5821 ggtcgtcggc tcgctggcgt tctgcgggct catcctgctc gggacctggg cggtggctgc
     5881 gctgggcggc aaggtgccgt tcgacggcgt gcggctcgct cagaccgtgc tcatcctgct
     5941 ggccgggacg gtggcgttct cgaccatggg tttctcgctg gcctattggt tccgccccaa
     6001 ggcggcgtca gcgatcggca atctcgtctt cctgccactg tcgttcctct ccggcttctt
     6061 cttccccctc gaccaactcc ccgacgtact ggccaaggtg gcgccctatc tgccgaccta
     6121 ccacttcggg caactcgtct ggtcggcgat cgccccggcg caggacgtca tcgccttcgg
     6181 cgcccatcct gaaggcagtg cctgggtgca cgtcggctgg gtcctcgcgt cgttcgtcgg
     6241 gttcggcctg ctggctcgcg ccggctaccg ccgcgagttg tcccgcgccg cccagtagtg
     6301 cggcgggcag caagcgccgg ggcagcagcg cggcgggcgg cactgtgcga gcgccgtccg
     6361 tggcaggctg aaggcatgac cgagaccccc gccgcagccg ctcctgtcgg tgacgacctc
     6421 tccctgtcgc acgccctgct catcgacgcc ttcggtcggg tcgccgaggg cgtccccgcc
     6481 ctcctcgacg gcctcggcgg cgaagagatc ctgtggcggc ccgacgagga ctccaactcc
     6541 atcggctggc tcgtctggca cctcgcccga gtgcaggatg accacctcgc gggagtggcc
     6601 gggcgcgaac agatctggac gacagccggt ttcgccaagc gcttcgacct cccgtatgcc
     6661 gcccgctcaa tcgggtacgg ccagtccagc caggaggtcg cggccttctc ggtcgccgac
     6721 gggagcctgc tcgcggcata ccatgcggcg gtccacgccg agacggtgga gatcgtgcag
     6781 ggtttgaccg cagccgactt cgcgcggatc gtcgaccggc gctgggaccc gccggtcacc
     6841 gcggccgtgc gcctggtgtc ggtcgtcaac gacatcaccc agcacgctgg acaggccgcc
     6901 tacttgcggg gtctggtcga acgccgccgc tcgtgagcgt gggcggggac gtcgcggggg
     6961 tatgccgttc gcctcaggac tacgcgaggg gcggcaagga ctcctaggct gcctaggtgc
     7021 tcaccgcgtt cgccgtcctg ctgtgtctgt cggcactcct cgcctgggcc aacgaacgtt
     7081 gggttcacct gcccagcacg gtggccgtca ccctcgcggg agccgcctca tcgatcgtgc
     7141 tcatcctgct cgacacgcaa ggctggacct tcgggctgaa gcagcaggcg atcgagctct
     7201 ggcaggcgct ggacttcacc gctttcgtcc tcaacggcat cctcagcctg atgctcttcg
     7261 cgggagcgat gagcctggac gccacactga tgatgcgcct gcgggccggc atcctcacct
     7321 tcgccggggt gagcaccgcg atcagcaccg tgctcatcgg cttcggcgcg tggggcgtct
     7381 tccacctggt cgggctcgac atcccgctca tctggtcgct gctcttcggt gcgctgttgt
     7441 cgccgaccga ccccgtggcc gtgctcgaca tgctcaagcg cgccaaggtg cccaagaaga
     7501 tcgagaccct catcgccggg gagagcctct tcaacgacgg cgtcggcgtc gtcttcttca
     7561 tcgtcattgc ctccgccgcg ggggtcggtg gcggccacac cgagcccagt ctcacgggag
     7621 cgttggtcgt cttcgtccaa gaggcgttgg gcggcatcgc cttcggcgcc ctgatcggct
     7681 ggatcaccta tcggctgtgt cgctcgatcg aggcggcgtc ggtcgagatc ctgctgaccc
     7741 tggcgtcggt cgtcggcggc tatgtcctgg ccgcgggcct cggcctcagc ggacccctgg
     7801 cgatggtcgt cgccggtctg gtgatgtcgg ccggcaaaga gcgagcgttc ggagagcaca
     7861 cccgggaacg gatcgaaggc ttctgggaga ccctcgacga ggtcctcaac atcctgttgt
     7921 tcgcgttcat cggtctcgac gtgctgctca cgcctaccgc cgtcggcgaa gtgctcgccg
     7981 ggctcctcat gatcgtcgtg gccctcgtcg cgcgatttgt cagtgtcgcg ctgccgatga
     8041 ccctggtccg ccgccgggac ggctacgggc cgtggacgat tcgggtgctc acgtggggag
     8101 gcctgcgagg cggcatcgcg atcagcctcg ccctcgggct cccggccagc ccctaccggt
     8161 ccgctctggt caccgccacc tacgtcgtcg tcctcttcac gatcgccgtc cagggcctga
     8221 ccgtcatgcc gatcgtccgt cgcgccgcgg cggccgaggc ggcggccgag gcggcggcgg
     8281 gcgagactga gaatcgcgct gctcgtccgt gacgtcaggc tgaggcacgt cgttttcgcg
     8341 ggtgaccacg atcggcgtcg actcgtggaa ccgaatgcac cggcatacca tcgaagaaga
     8401 cggtcgggac aggagggcca tgctgaccgt attcatcgcc atcggcgctg ccggactggc
     8461 actgctcgtg atttcgctgg tcgtcggcga gattcacggc ggccttttcg agtggatcga
     8521 caacgacctt ttctccggcg ccgcggtgtc ggccttcctg tccgcgctcg gtttcgtcgg
     8581 cgcgctcgtc gaaagcacct cgggcaacac caccgtcgcg actctcgcgg gcacgggggc
     8641 cggcgtgggc atggcgtatg ccgcccgcgg tctcacgcgc tcgctgcagt ccggcgaggg
     8701 agacaccacg gtgcgttccg gcgacctcgt cggccaccga ggtcatgtcg tcgaggcgat
     8761 tccccgcgat ggcatgggca tggtcagcgt gagcgtcgct gggcacctca cccggctcaa
     8821 cgcccgctgc gaacagccgc tctccgcggg aaccggaatc gaggtcacct ccgtcctatc
     8881 cccgacggca gtgacggttc gacccattgt cgtcacccct caggatcgcg cgctcgactg
     8941 agcgcgcata gccgccggaa aggcgcgaac aatgtctgcc aatgagtttc ctgtcatcct
     9001 cgttgccatc gtcgggctga tcatcctgct cgtcgtggtg ggggtgctcg tcgcctcgcg
     9061 ctacaaggtg gccgggccaa acgaggcctt catcgtcacc ggacgcaagg gcaaggagat
     9121 ccgcgacgtc gaaaccggtg aggtctccac cgatctgtcc gggcagaagg tcgtcatggg
     9181 tggcggcatt ttcgtcgtgc cgttcgtcca gcgcctgcac gtcctggacc tctccagccg
     9241 ccggatcatg atccagatcc gcggcgccgt ctcgggccag ggcgtcaagc tcaacctcga
     9301 cggggttgcc ctcgtcaagg tcggcggcaa cgaagactcg atccgcgcgg cggcccaacg
     9361 cttccttacc cagcagcgcg agatcgagac cttcacgcag gagaccctgg cgggctcgct
     9421 gcgctcgatc gtgggttcac tgaccgtcga gcagatcatc cgtgaccggg ccgccttcgc
     9481 ccagcgagtg gccgacgagt cggagacctc gcttaccggt cagggtctgg tccttgacac
     9541 cttccagatc caggacatca ccgacgacgg gagctacctc tccgacctcg gccgccccga
     9601 ggccgccagg gttgggcaga cggcggcgat cgccgaggcc aacgcccgac aggctgcgga
     9661 gcaggctcgg ctggccgccg agcaggagat cgccatagcc aaccgtgagt tggccctcaa
     9721 gcaggccgag atcaaggcgc agaccgacgc ggccaccgct caggctgccg ccgccggccc
     9781 cctggctcag gccgaccgcg accaggcgat ccttgccgag caggagaagg tcgccgtgcg
     9841 caaggccgcg ctcaccgagc gccagttgga caccgaggtt cgccgcccgg ccgacgccgc
     9901 ccggtatcgc gtcgagcagg aggccgacgc ccgcaaggcg gcctcgatcg ccgcggccca
     9961 ggccgacgcc gagaaggccc ggctcacagg tgaaggcgag aaggctcggc gcaccgccga
    10021 ggccgacgcc ctgcgagccg aaggcaccgc ccaggccgcc gcgatcctcg ccaccggcga
    10081 ggccgaggcc caggcgatga acaagcgcgc cgacgcctac gcccgctaca acgaagccgc
    10141 cgtcctgcag atgctcgtcg aggtcctgcc caaggtcgcc cgcgagctcg cctcgcccat
    10201 ggcggcgatc gacaacctca ccgtcatctc caccgacggc gccggggccc tgcccaagca
    10261 ggtgacggcc aacctggtcc agaccatgtc gatgctcaag agtgcgaccg gtttcgacct
    10321 ggaggacatc gtcaagcggt actccgggtt cgtcccggct cccgccacgt cggtgacggc
    10381 ggccacgacg gcccctgcgg caccggcggc cgagaccgcg ccgcccgcca cgcctgccac
    10441 ctccgcctga cgggcgcagg ctaggggctc gcgctcgagt tctttctgct cgagccgggt
    10501 cctggtcgcc cgggcggacg ctgcggatcg ttacccgttt gtgttgcgtt tgcctgtgga
    10561 agcggcggtt tggggtgggt ggcctgtcgg tggtcgctgt tagcgtcggg gttatggaca
    10621 gca
//
LOCUS       DAORPW010000078        10434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1456_length_10434_cov_870.218325, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000078 DAORPW010000000
VERSION     DAORPW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10434
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1456_length_10434_cov_870.218325"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1470)
                     /locus_tag="PLB86_07710"
     CDS             complement(19..1470)
                     /locus_tag="PLB86_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRC65062.1"
                     /translation="MAKSARASRRGLRWAIAGSVAVALVASTATGASADPTPVVPSAE
                     QVSAAAAAAGSAEAQVAALDAELAVARATLQEQQERAGAAAEDYNRAAEELDEATSAH
                     TTATAKATAAAQASEEATLALSRYAAEVFQSGGGLGQLDVFFGGGGPAQTLDRAAGLE
                     AVGEERARVMRDAEAARHLARTLAEAAAEAQQRQEQAAQTAHAARDIAQRDNDAAAAA
                     AATAAAHQEQALGQLATLRNTSVELERQRQDGLQAIEQARIAEEARRKAEEAARQAAA
                     QAARLAAAEAARLAEADRQAAAAAAEAARKAAEEAAKKATSTPSPTPTPKPSTTPTPK
                     PSTTLTPTPTPTKSTTPPPTPPPATNVPAPKAGVDAVIAFARAQLGEPYVWAAAGPNS
                     WDCSGLTMVAWAQAGVRLTHYTGSQYKQTARVPVDQLKPGDLVFFGPSVAGIHHVGLF
                     IGNGQMIHAPYTGTVVKISTIWRPDLIPYGGRP"
     gene            complement(1752..3086)
                     /locus_tag="PLB86_07715"
     CDS             complement(1752..3086)
                     /locus_tag="PLB86_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HRC65063.1"
                     /translation="MDTDHLWPEPLRRMPDGTVKQINPYSGTSVWTVPGRGNRPIASG
                     LPAPGPIDPAMEGRHCAFCEGRYFDTPPEKSRLVADPDGWHLLRGLTANRIEDSVAQF
                     RRIPNLFEIVAYDYWRLNYGFEPGSEARERHDHYLSTDLGRKHVDQMIRTRAMAGGMS
                     EAQWAALTPQDKAQRAMSFFAGTHDVIVARRHFVDGASTTDQLAASGSLTPDEHAAYL
                     RFTVDALRSLYDANRYARYVAVFQNWLRPAGASFDHLHKQLVAVDERSVRTEHELRRL
                     RDNLNLYNEFAVDYAARQNLIVAENPYAVAFAGFGHRYPSLEIYSKSEFARPWEQPSD
                     DLRGFSDLLHACHAATGAAVPSNEEWHYQPPDVDLPMPLRTVLKWRVSTLAGFEGGTK
                     IYLNTIDPWHLRDRVVGALFELREQGRIAPDIRIATECACRPNSLKYNPLLH"
     gene            complement(3268..4287)
                     /locus_tag="PLB86_07720"
     CDS             complement(3268..4287)
                     /locus_tag="PLB86_07720"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HRC65064.1"
                     /translation="MKLHEFPRHPLTFGPSPVHPLRRLTQHLSGAQIWAKREDVSSGL
                     AFGGNKVRKLEYIVPDVLATGADTLVSIGGYQSNHTRQVAAVAAYLGLKCRLVQEKWV
                     PWDDPVNDKVGNILLSRMMGADSRLDPHGFDIGIRDSWKDALREVEASGGTPYPIPAG
                     ASEHRLGGLGFANWAFEVAEQEKALGVFFDTIIVCTVTGSTHAGMIAGFAALEDLTGV
                     RRRVIGIDASATLEQTRDQVGRIARHTSELIELGRDLRDDEITVLEGWAGELYGVPVD
                     STMEAIRLGAQLEAMITDPVYEGKSLAGLIDLVRSGDIPATSTVLYAHLGGQPAINAY
                     HRLWS"
     gene            4423..4695
                     /locus_tag="PLB86_07725"
     CDS             4423..4695
                     /locus_tag="PLB86_07725"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HRC65065.1"
                     /translation="MSTTAKDERLALRLTRSQRKALEAAATATGSTLTEFSVAAILAR
                     AEDVLGSRAVFEVDEQVWSEFVNALDQPVTSDRFAALIARPPVWDE"
     gene            4794..5189
                     /locus_tag="PLB86_07730"
     CDS             4794..5189
                     /locus_tag="PLB86_07730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC65066.1"
                     /translation="MRNQDSGASRTFVTTCDGLVVGYYALAASSVAQVGAPAGLRRNM
                     PDPIPVILLGRLAVDRRHAGRGVGASLLRDAALRVATTAGIVGVKAMLVHAIDDGAVA
                     FYLHHGLQPFPGDPTTLFVPVAAIRPPGM"
     gene            complement(5322..5394)
                     /locus_tag="PLB86_07735"
     tRNA            complement(5322..5394)
                     /locus_tag="PLB86_07735"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5359..5361),aa:Thr,seq:cgt)
     gene            5578..6861
                     /locus_tag="PLB86_07740"
     CDS             5578..6861
                     /locus_tag="PLB86_07740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRC65067.1"
                     /translation="MTLRGPLSLGTALAAGLSLTLSSCTGAGAPATLAGGSPTASATV
                     VAKPAASNAGTPAPAPGGTTPTPAPAPATPAKDGSTVTIALTGDVLLHSGVTDQAARD
                     GAANPAQASHGLDYRPLLAGAKPFVEGADLAICNLETPVAPPGGPYTSYPVFSVPPEI
                     LPALVDTGFDACTTASNHTVDKGVPGIQRTLDAMDAAGLKHAGSNRSAEEQSGTTMLD
                     AHGVKVALLAYAYGLNGFRLPADKTWAVDVIDTATILADARAARAAGAQIVLVALHAG
                     TEYQILPTAQQRDVATALAASPDIDLIYGHHAHVVQPFDKIGDTWVAYGLGNMIAHQH
                     RLPKLTMAQHEVMASFTFAQGSDGRWAVTRAEARPAAMSNLNEPLRYVDLATLLADPA
                     VSEARKAAYRPFYDRAVAALTKLGADGKGLVIAGR"
     gene            complement(6913..8538)
                     /locus_tag="PLB86_07745"
     CDS             complement(6913..8538)
                     /locus_tag="PLB86_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC65068.1"
                     /translation="MRLPTARPRSTKVDRSRVDRRLALVASGVVAVLSLSACLVLPFL
                     PNDPATPSGGSTTVPLSTADASAVRQPPAGTTGLDRFYAQQLAWSSCQGGECATLEVP
                     IDYAQPQGATIELALLRVKAKSSNGRLGSLVVNPGGPGGSGVEYASYANQIVGAPVRA
                     AYDIVGFDPRGVGDSAPIDCLDDAGLDAFLGMDPTPDDLAEEQAFVKGAKALADGCKA
                     RGGALLAHVSTVDAAKDMDILRAALGEAKLHYLGKSYGTFLGSTYAGLFPAKVGRFVL
                     DGVVPPDLTSSELSQGQARGFEEATRAYVANCIKRSSCPVGSTVDEGMQWLRDFLKGL
                     DGQPIRVTTDAKITELNEAWAAIGLANAMYSQQAWPSLTSALIAAQNGDGDELMDLAN
                     QYADRTSGGTYAGNIMEVIYAVNCLDRPDTADLTVIEKNADAFAQVAPTWGRFLAWGS
                     VPCGVWPIQTGNTPAKITAAGSGPIVIVGTTRDPATIYEWSVRLRGDLANSVLLTFDG
                     DGHTAYTRSNSCIDKPIDDYYVKGIVPTDGLKC"
     gene            complement(8632..9783)
                     /locus_tag="PLB86_07750"
     CDS             complement(8632..9783)
                     /locus_tag="PLB86_07750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRC65069.1"
                     /translation="MSVWDEVVGQSQTVRTLSHAATHPDAMTHAWLLTGPPGSGRSTA
                     ARAFAAALQCPNHGCGTCRECRTAVEGTHADVRIVATEGLSIKVEDTRALVQEAGLRP
                     SVGAWRVIVIEDADRLTERAADALLKALEEPTPRTVWILCAPSLEDVIVTIRSRCRHV
                     RLRTPPVAAVAELLTRRDGIPEPMARYAARAAQCHVGKARRLARDEGARNRRHEIISL
                     PLRIRGVGDAVGAAADLASIADAEAGASSSSRDAAERTRLLETLGADPEARTQPPHVR
                     AQLAALEKEQKARATRFGRDVVDRSLVDLLSMYRDALVVRAGATVELVNAEALDQVRA
                     LAAAMSAERLLACMDAVGEARERINLNVPPLLALEAMAVSLQVPTPSSR"
     gene            complement(9780..10421)
                     /gene="tmk"
                     /locus_tag="PLB86_07755"
     CDS             complement(9780..10421)
                     /gene="tmk"
                     /locus_tag="PLB86_07755"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRC65070.1"
                     /translation="MSLPSTTTPRLGLFVAFEGGDGAGKSTQVRLLAQALTNAGREVV
                     VTRQPGGTPLGSAIRDLVLHGGHVSPRAEALLYAADKAHHVDELIRPTLERGVDVLTD
                     RYTDSSIAYQGAGRDLGPQEIHELLVWAVAGLFPDLTIVVDVPAAVGRARRGSVHDRI
                     ESEGDDFHERVRQHFLDLAAAAPQRYLVLDGTADPADLHAQVVARLRAEGVAL"
BASE COUNT         1501 a         3667 c         3684 g         1582 t
ORIGIN      
        1 cgctacggga gggcccggtt agggacggcc gccgtagggg atcaagtcgg ggcgccagat
       61 cgtggagatc ttgacgacgg tccccgtgta gggcgcgtgg atcatctggc cgttgccgat
      121 gaagagcccc acatgatgga tgcccgcgac gctggggccg aagaagacca ggtcacccgg
      181 cttgagttgg tcgacgggca cgcgggcggt ctgcttgtac tggctcccgg tgtagtgcgt
      241 gagccgcact cccgcctgcg cccaggcgac catcgtcagg cctgagcagt cccacgaatt
      301 ggggcctgcc gcagcccaga catacggctc gccgagttgt gcgcgggcga aggcgatcac
      361 ggcatccacg ccggccttcg gcgccggcac gttcgttgcc ggcggcgggg tcggcggggg
      421 cgtggtggac ttcgtcgggg tcggcgtggg agtcagggtc gtcgacggct tgggcgtcgg
      481 ggtcgtcgac ggcttgggcg tcggggtcgg gcttggggtc gaggtcgcct tcttggccgc
      541 ctcctccgct gccttgcggg cggcctcagc cgctgctgcc gcggcctggc gatccgcctc
      601 ggccagccgg gcagcctctg ccgccgcgag gcgagcagcc tgcgctgcgg cctggcgggc
      661 ggcttcctcg gccttgcgtc gggcctcctc agcgatcctc gcctgctcga tcgcctgcag
      721 gccgtcctga cgctggcgtt caagctcgac ggacgtgttg cgcaacgtcg ctagttggcc
      781 cagcgcctgc tcctgatggg cggccgcggt cgccgctgct gccgcggccg cgtcgttgtc
      841 acgttgggcg atgtcgcgtg ctgcgtgggc cgtctgcgcg gcctgctcct ggcgctgctg
      901 tgcctcggcc gcggcttccg cgagcgtacg ggccaggtgg cgtgcggctt cggcgtcgcg
      961 catgacccga gcgcgctctt cgccgacggc ttcaaggcca gcggccctgt cgagcgtctg
     1021 agcgggcccg ccccctccga agaagacgtc cagttggccg agtccgccac cgctttggaa
     1081 cacctcggcg gcataccggg acagggcgag ggtcgcctcc tcggaggcct gggcggcggc
     1141 ggtcgccttg gcggtggccg tcgtatgcgc cgacgtggcc tcatcgagtt cttcggcggc
     1201 gcggttgtag tcctccgcgg ccgctccggc ccgctcttgc tgctcctgca gggtggctcg
     1261 ggcgacggcc aactcagcgt cgagtgcggc gacctgagcc tccgccgaac cagcggcggc
     1321 cgcggcggcc gagacctgct cagccgacgg cacgaccggt gtcgggtctg ccgatgctcc
     1381 ggtcgcggtg ctcgcgacca gagcgacagc cacgctccca gcgatggccc agcgcagccc
     1441 gcgtcgcgac gcgcgcgctg acttcgccac gccacacgct ccttcgctgc cgttgacgag
     1501 cacccgaatt gcctacgacc ctctccccca ggccgttgcc gagcgtagaa cccattcgtc
     1561 acacgcgaaa cagcgatcac tccaataccg ttgcgacacg ccgtgattca aaaatgacag
     1621 ccgtgtgatt ggtcgaatca cacggctgtt ttttcgcagg ttatcgagcg aattcgctcg
     1681 cctgttcgaa tgagataacg atatggtaac gaaaggcggg gtgcgtggcc ggtcggacgc
     1741 cggccaggcc ctcagtgcag cagcgggttg tacttcaggg agttcggccg gcaggcgcac
     1801 tcggtggcga tccggatgtc cggggcgatc cgtccctgct cgcgcaactc aaacagggca
     1861 ccgaccaccc ggtcgcgcag atgccagggg tcgatcgtgt tgagatagat cttggtcccg
     1921 ccctcgaagc ccgcgagcgt cgagacccgc catttgagca ccgtccgcag gggcatcggc
     1981 aggtcgacat ccggcggctg gtagtgccac tcctcgttgc tgggcaccgc cgctcccgtc
     2041 gccgcatggc aggcgtgcag gaggtcggag aagccgcgca ggtcgtcgga gggctgctcc
     2101 cacggccgcg cgaactcgct cttgctgtag atctccagcg acggataccg gtggccgaag
     2161 ccggcgaagg ccacggcata cggattctcc gcaacgatga ggttctgccg ggccgcgtag
     2221 tcgaccgcga actcgttgta gaggttgagg ttgtcccgca ggcggcgcaa ctcgtgctcg
     2281 gtccgcacgc tgcgctcgtc gacggcgacc aactgcttgt gcaggtggtc gaaggacgcg
     2341 cccgcgggcc gcagccagtt ctggaagacc gcgacgtagc gcgcatagcg gttcgcgtcg
     2401 tagagcgagc gcagcgcgtc gaccgtgaag cgcaggtatg ccgcgtgctc gtcaggggtg
     2461 agcgaccccg aggcggcgag ttgatcggtc gtgctcgctc cgtcgacgaa atgccggcgc
     2521 gcgacgatga cgtcatgcgt cccggcgaag aacgacatgg cccgctgggc cttgtcctgg
     2581 ggagtcagcg ccgcccattg cgcctcgctc atgccccccg ccatggctcg cgtgcggatc
     2641 atctgatcga cgtgcttgcg ccccaagtcg gtcgagaggt agtggtcgtg ccgctcgcgc
     2701 gcctcgctgc ccggctcgaa cccgtagttg agccgccagt agtcataggc gacgatctcg
     2761 aacaggttgg ggatccggcg gaattgcgcg accgagtcct cgatccggtt ggcggtcagc
     2821 ccccgcagca ggtgccaccc gtcggggtcc gcgaccagcc gtgacttctc cggcggcgtg
     2881 tcgaagtagc ggccctcgca gaacgcgcag tgccgcccct ccatcgcggg gtcgatcggc
     2941 cccggcgcag gcagccccga agcgatcggc cggttgccgc gccccggcac ggtccagacc
     3001 gaggtccccg agtaggggtt gatctgcttg accgtcccgt cgggcatccg acgcagcggc
     3061 tccggccaga ggtggtcggt gtccatcaca tcggccagag tagccagggg gtgctcatcg
     3121 gcccgggccc tcagtcccga cccgcgggtg ccgcgcgcac acggcatacc aatggtcggg
     3181 ccgcgcgccg cgactgagca cgggtatgcc gcccgccccc gaccaatggc attcggtgtg
     3241 ttccgcgggg cggcgccccc cggacgctca ggaccagagg cggtggtagg cgttgatcgc
     3301 cggctggccg ccgaggtggg cgtagagcac ggtggaggtc gcggggatgt cgccgctgcg
     3361 gaccaggtcg atgaggccgg cgagcgactt gccctcgtag accgggtcgg tgatcatcgc
     3421 ctcaagttgg gcgcccaggc ggatcgcctc catcgtcgag tcgaccggca cgccgtagag
     3481 ctcgcccgcc cacccctcca ggacggtgat ctcgtcatcg cgcaggtcgc gtcccagctc
     3541 gatgagctcg gaggtatgcc gcgcgatccg gccgacctgg tcgcgggtct gctcgagtgt
     3601 cgccgaggcg tcgatgccga tgacgcggcg ccgcacgccg gtgaggtcct ccaacgcggc
     3661 aaagccggcg atcatcccgg cgtgggtcga gccggtgacc gtgcagacga tgatcgtgtc
     3721 gaagaagacg cccagggcct tctcctgctc ggcgacctcg aaggcccagt tggcgaatcc
     3781 gagcccgccg agccggtgtt cagaggcgcc ggccgggatc gggtagggcg tgccgccgct
     3841 ggcctcgacc tcgcggaggg cgtccttcca cgagtcacgg atgccgatgt cgaagccatg
     3901 cgggtccagg cgcgagtcgg cgcccatcat ccgcgagagc aggatgttgc cgaccttgtc
     3961 gttcaccggg tcgtcccagg ggacccactt ctcctggacg aggcggcact tcaggccgag
     4021 gtatgccgcg accgccgcga cctggcgggt gtggttggac tggtagccgc cgatcgagac
     4081 cagggtgtcg gcgccggtgg ccagcacgtc ggggacgatg tattcgagtt tgcggacctt
     4141 gttgccgccg aaggccaggc cgctgctgac gtcctcgcgc ttggcccaga tctgggcgcc
     4201 gctgaggtgt tgggtcaggc ggcgcagcgg atgcacgggg ctgggaccga aggtgagcgg
     4261 gtggcgcggg aactcgtgca gcttcatgac gactcctcgg gagtggagcg accgaccgct
     4321 cgtttcgggg gccaggggcg cgtctggtgc tgcaatatat cgcgcttgag gttggggaat
     4381 ccgggaactc ggccgtacgc cttagaggcg tacactgggc cgatgagcac cactgccaag
     4441 gacgaacgcc tcgccctgcg cctgactcgc tcgcaacgca aggcactcga agcagcggcc
     4501 accgccaccg ggagcaccct caccgagttc agcgtcgccg ccatcctggc gcgggcggag
     4561 gacgtccttg gctcgcgcgc ggtcttcgag gtcgacgagc aggtctggag cgagttcgtc
     4621 aacgcgctcg accaaccggt gacctcagat cgcttcgccg ccctcatcgc ccgaccgccc
     4681 gtgtgggacg agtgagtcgc tacacgccgc ctgagcccct gcgaccaacc cacgatctgg
     4741 ctggcttcga cagcgggaac gacgcactga atcgctggct ggtcagtcga gccgtgcgta
     4801 accaggacag cggcgcctcc cgaaccttcg tcaccacctg cgacgggctc gtcgtgggct
     4861 actacgccct ggccgcatcc tccgttgctc aggttggggc ccccgccggc ctgcgacgca
     4921 acatgcccga cccgatcccc gtcatcctcc tcggccgctt ggcggtcgac cggcgacatg
     4981 ctggccgcgg agtcggtgcc tcactgttgc gcgacgccgc tctccgggtg gcgacaacgg
     5041 ccggcatcgt cggggtcaag gcaatgctcg tccacgcgat cgatgacggg gccgtcgcgt
     5101 tctacctcca ccacggtctg cagccgttcc ccggcgaccc gacgaccctg ttcgtccccg
     5161 ttgcggcgat ccgccctcca ggcatgtgag ccggccgcga ggcgagcgag ttgcgtcatg
     5221 ccagacgcgc ggcatacgtc gcggttggca tgacgcaagc cggggcgagc ggggcgggca
     5281 gcagaaacgc cccccggggt gagcccggag ggcgttgtct gagccgcctt ggggaatcga
     5341 accccagacc tattcattac gagtgaatcg ctctgccgac tgagctaagg cggcgtgcgc
     5401 cccagccgag gcgcgggcac cgagtatacg gacaccggcg ccaccccacg aaaccggttg
     5461 cctgaggcgc gcggcgtacc cctcccgcgg caggcctctg gcgaggcgcg tcacccccgg
     5521 ttcgtgtcac acccggaagc ccccggtatg ccgcgagcgt ggcttagtct cggatccgtg
     5581 accttacggg gccctctctc cctcggcacg gcgctagccg ccggcctgtc cctcaccctg
     5641 tcctcctgca ccggagcagg agcgccggcg accctggctg gagggtcccc caccgcctcg
     5701 gcgaccgtcg tcgcgaagcc ggccgcctcg aacgcgggca cgccggcccc cgcaccgggc
     5761 ggaaccacac cgacgcccgc accagcccca gccacaccgg ccaaggacgg ctcgaccgtg
     5821 accatcgccc tcaccggcga cgtcctgctg cactcagggg tcaccgacca ggcagccagg
     5881 gacggcgcgg cgaacccggc ccaggccagc cacgggttgg actaccggcc gctgctcgcc
     5941 ggggccaagc cgttcgtcga aggtgctgac ctggcgatct gcaacctcga gacgcccgtt
     6001 gcaccgccgg gtggtcccta caccagttac cccgtcttct cggtcccgcc ggagatcctc
     6061 ccggcgctcg tcgacaccgg gttcgacgcc tgcacgaccg cgtccaacca cacggtcgac
     6121 aagggtgtcc ccggcatcca gcgcaccctg gacgcgatgg atgccgctgg cctcaagcac
     6181 gccggcagta accgcagcgc cgaggagcag tccggcacga cgatgctcga cgcccacggg
     6241 gtcaaggttg cgctgctcgc ctacgcctac ggcctcaacg gtttccgact ccccgccgac
     6301 aagacctggg ccgtcgatgt catcgacacc gccacgatcc ttgccgacgc tcgtgcggcc
     6361 cgcgcggccg gtgcccagat cgtcttggtc gccctgcacg ccgggacgga gtatcagatc
     6421 ctccccacgg cccagcagcg cgacgtcgcc accgcgttgg cggcgtcacc ggacatcgac
     6481 ctcatctatg gccaccacgc ccacgtcgtg caaccgttcg acaagatcgg cgacacctgg
     6541 gtcgcctacg ggctaggcaa catgatcgcc caccagcacc ggctccccaa gctcaccatg
     6601 gcccagcacg aggtcatggc ctcgttcacc ttcgcccagg gcagcgacgg gcgctgggcc
     6661 gtcaccaggg ccgaggcccg gccggccgcg atgagcaacc tcaacgaacc gctgcggtac
     6721 gtcgacctgg ccaccctgct ggccgacccc gccgtgagcg aggcgcgcaa ggcggcatac
     6781 cggccgttct atgaccgcgc cgtcgccgct ctcaccaaac tgggagccga cggcaagggc
     6841 ctggtcatcg ccggccgctg accgcgccac cgcacccgcg acccgacgcg cgagcgaacc
     6901 gcgcggcata cctcagcact tgaggccgtc ggtcggcacg atccccttga cgtagtagtc
     6961 gtcgatcggc ttgtcgatgc acgagttgga ccgggtgtat gccgtgtgcc cgtcaccgtc
     7021 gaaggtgagc agcaccgagt tggcgaggtc accgcgcaac cggaccgacc attcgtagat
     7081 cgtcgccggg tcccgggtgg tgccgacgat gacgatcgga ccgctgcccg ccgcggtgat
     7141 cttggccggc gtgttgccgg tctggatcgg ccagacgcca cagggcaccg aaccccacgc
     7201 caagaaccga ccccacgtcg gcgcgacctg ggcgaaggca tcggcgttct tctcgatgac
     7261 cgtcagatcg gcggtgtcgg gccggtccag gcagttgacc gcgtagatga cttccatgat
     7321 gttcccggcg taggtcccgc ccgaggtgcg atcggcgtac tggttggcca ggtccatgag
     7381 ttcgtcgccg tcaccgttct gggcggcgat cagggcggac gtgagcgagg gccaggcctg
     7441 ctgggagtac atcgcattgg ccagtccgat ggcggcccag gcctcgttga gctcggtgat
     7501 cttcgcatcg gtggtcaccc ggatcggctg cccatccagc cccttgagga agtcacgcaa
     7561 ccactgcatg ccctcatcga cggtcgatcc gaccgggcag gagctgcgct tgatgcagtt
     7621 ggcgacatac gcgcgagtcg cctcctcgaa gccgcgggcc tggccctggg acaactccga
     7681 ggaggtgagg tccggcggca ccacgccgtc gaggacgaag cggccaacct tggccgggaa
     7741 caacccggca tacgtcgatc cgaggaaggt gccgtaggac ttgccgaggt agtgcagctt
     7801 cgcctcgccg agcgctgccc gcaggatgtc catgtccttg gccgcatcga ccgtcgagac
     7861 gtgcgcgagc agcgcgccgc cgcgcgcctt gcacccgtcg gcgagcgcct tggccccctt
     7921 gacgaaagcc tgctcctcgg ccaggtcgtc cggcgtcggg tccatcccga ggaaggcgtc
     7981 cagacccgcg tcgtcgaggc agtcgatcgg agccgaatcg cccaccccgc gcgggtcgaa
     8041 accgacaatg tcgtatgccg cgcgcaccgg cgcgccgacg atctggttgg cgtagctggc
     8101 gtactccacc ccggaaccgc ccggcccgcc agggttgacg accagcgacc cgaggcgccc
     8161 gttggaggac ttggccttga cccgcagcag cgcgagctcg atcgtcgccc cttgcggctg
     8221 ggcgtagtcg atcggcacct cgagggtcgc gcactcgccg ccctgacagg agctccaggc
     8281 cagttgctgg gcatagaagc ggtcgaggcc ggtcgtcccc gcaggcggtt gccggaccgc
     8341 gctcgcgtcg gccgtcgaca acggcacggt cgtgcttccg cccgacgggg tcgccgggtc
     8401 attcggcagg aacggcagca ccaggcacgc cgagagcgac agcacggcca ccacgcccga
     8461 cgcgacgagg gccagccgcc ggtcgacccg gctgcggtcc accttcgtgc tgcggggccg
     8521 tgcggtcggg agtctcatga ccggtggtgc tccttctcgt ccactcaagc gggcggcata
     8581 cccaatcgtc cggaacgaac cgacccggac atctgccgtc ttggcggaag gttacctgct
     8641 ggagggggtc gggacctgga gtgagaccgc catggcttcc agcgcgagca gcggcgggac
     8701 gttgaggttg atccgttcgc gcgcctcgcc gaccgcgtcc atgcaggcga gtagccgctc
     8761 ggcgctcatc gcggcggcca gcgctcgcac ctggtcgaga gcctcggcgt tgacgagttc
     8821 gacggtcgcc cccgcacgga cgacgagcgc gtcgcggtac atcgacagca ggtcgacaag
     8881 cgaccggtcg acgacgtcgc gcccgaagcg ggtcgcgcgg gccttctgct ccttctccag
     8941 ggccgccagt tgggcccgga catggggcgg ctgagtgcgt gcctccgggt cggccccgag
     9001 ggtctccagc agccgggtgc gctcggcggc gtcccggctg gacgacgacg ctcctgcctc
     9061 tgcgtcggcg atgctcgcga ggtcggcggc ggccccgacc gcgtcgccca ccccccggat
     9121 gcgcagcggg aggctgatga tctcgtggcg ccgattacgg gccccctcgt cgcgggccag
     9181 gcggcgcgcc ttccccacat ggcactgcgc cgcccgagcg gcatacctgg ccatcggctc
     9241 cggtatgccg tcccgccggg tcagcaactc ggccaccgcc gcgacgggcg gggtgcgcag
     9301 gcggacgtgg cggcagcgcg accggatcgt gacgatgacg tcttcgaggg agggggcgca
     9361 gaggatccag accgtgcggg gcgtgggctc ttcgagggcc ttgaggaggg cgtcggcggc
     9421 gcgttcggtg aggcggtcgg cgtcctcgat gacgatgacc cgccaggccc cgaccgacgg
     9481 gcgcagcccg gcctcctgga ccagggcgcg ggtgtcctcg accttgatcg acaggccctc
     9541 ggtggcgacg atccgcacgt cggcgtgggt gccctcgacg gcggtgcggc actcccggca
     9601 ggtgccgcag ccatggttgg ggcactgcag cgccgccgcg aaggcccggg ccgcggtcga
     9661 tcggccggag ccgggtgggc cggtgagcag ccaggcgtgg gtcatcgcgt cggggtgcgt
     9721 ggcggcgtgg ctgagcgtcc ggacggtttg ggactgaccg acgacctcat cccagacgct
     9781 cacagggcga ccccctcggc gcgcaggcgg gcgacgacct gggcatgcag gtcggccgga
     9841 tcagcggtgc cgtccaacac gaggtagcgc tgcggggcgg ccgcggccag gtccaggaag
     9901 tgctggcgga cccgctcgtg gaaatcgtcg ccttcggact cgatgcggtc gtggacggat
     9961 ccgcggcggg ctcggcccac ggctgctggc acgtcgacga cgatcgtcag gtcggggaac
    10021 aacccggcga ccgcccagac cagcagctcg tggatctcct ggggccccag atcgcgcccg
    10081 gcaccctgat aggcgatcga ggagtcggtg tagcggtcgg tgagcacatc gacgccgcgt
    10141 tcgagggtcg ggcggatgag ttcgtcgaca tggtgagcct tgtcggcggc atacagcagg
    10201 gcctcagcgc gcggcgagac gtgcccgccg tgcaggacga ggtcgcggat cgcgctgccc
    10261 aggggcgtgc ctccgggctg gcgagtgacg acgacttcgc ggcccgcgtt ggtgagggcc
    10321 tgcgcgagca gccgcacctg ggtggacttc ccggccccat cgcccccttc gaaggcgacg
    10381 aacagcccca gtcggggcgt cgtcgtcgag ggaaggctca cgtgccagag ccta
//
LOCUS       DAORPW010000079        10294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1487_length_10294_cov_582.548393, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000079 DAORPW010000000
VERSION     DAORPW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1487_length_10294_cov_582.548393"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLB86_07760"
     CDS             <1..219
                     /locus_tag="PLB86_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRC65071.1"
                     /translation="FAFDKVAFEAISAFATVGLSTGITPTLPGIGQLILMALMYVGRV
                     GTVTTAVALALNTRHRHYRLPEERPIIG"
     gene            212..919
                     /locus_tag="PLB86_07765"
     CDS             212..919
                     /locus_tag="PLB86_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002856329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRC65072.1"
                     /translation="MVSNPFRRDDENPPVIIIGLGRFGVSVARSLVAMGQEVMAVDLD
                     EVRVQRYADEFTHVVQADSSDRDALEQLGVESFDKAVIGISSNVESSVMTALALVDYE
                     IPDIWAKASTKTQGRILERLGVHHVVYPERAMGEQVAHMIVGGMTQYLEFDDDFAIAR
                     TMAYHEQWGKTFAETGVRAKYGVTIVGVKRIGEDFIYARPETLVQPQDELVVSGPTKN
                     VEHFCALSKPKIARQPH"
     gene            1064..1483
                     /locus_tag="PLB86_07770"
     CDS             1064..1483
                     /locus_tag="PLB86_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRC65073.1"
                     /translation="MTSPVDVLTEVECWAVLRRQELGRLAFRHGDGLQITPVNFAVAG
                     DHVVFRTGDGGKFSDLVQNPEVAFEVDATVGEVAESVVCRGVVVELTGEQALMTDQLR
                     LRPWVASHKTHVMAIRVSEITGRRFHLTKPWSHIRPV"
     assembly_gap    1655..1754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1755..2743)
                     /locus_tag="PLB86_07775"
     CDS             complement(<1755..2743)
                     /locus_tag="PLB86_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65074.1"
                     /translation="MSRKRASDKTEDLSVFGRLAAARGERGDGTVFGALAQLRESVAP
                     AAQRGRTRAMQAAAPLADRAREVATPLAERAKDATAPYAGRARDAADAAREAAAPYAG
                     RAREAAAPYAGRAREAAAPYAGRARDAAEAAREAAAPYAEKAKEAAGAAREAAAPYAE
                     RAKEAAEAGREWARPRVEHGVEVAAPRLQAAVDEIAPKVDQARDSLVEDLLPKVSAAI
                     AAVAAAAGSAKRDLAAGAPQAFAAATEAVSDAVSSTDAPAVAGSAGKAKGKSKKGGLL
                     LLFGIGAAAAAAIAVIMSRKPKDDPWAAPVTETAYLPPTTGRASTVAPVADQP"
     gene            2981..3502
                     /locus_tag="PLB86_07780"
     CDS             2981..3502
                     /locus_tag="PLB86_07780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC65075.1"
                     /translation="MKATLHTNHGDIVVTLFPNEAPKTVANFVGLATGEKDYRDDAKR
                     TNPTKFYDGLVFHRIISGFMIQGGCPLGQGYGGPGFTFDDEPHPDKTFDRPYLLAMAN
                     AGKRMGKGTNGSQFFITVSTPGYLNFKHTIFGEVADAPSRAIVDAIAAVRTDRNDRPL
                     EPVVIESVTVDQD"
     gene            3707..4462
                     /locus_tag="PLB86_07785"
     CDS             3707..4462
                     /locus_tag="PLB86_07785"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRC65076.1"
                     /translation="MDCVRESARTVRPTRTAFGGRTIDGNPLATKAIIAICAAVYVLQ
                     FLRPVTTEDLAFVPVIGWHEPWRALTGAFLHSPSTVLHLGLNMLMLWQIGPYLESLLG
                     RARFLALYLVSAVGGSAGVLLLAPAPRSILFTSAEAAQYAAWATPVVGASGAVFGLFG
                     ALLVLNRHLGRSSTPLYAVLAVNIVFGFIYPGIAWQAHLGGLVTGTAVAGVFAVLAHR
                     DRRSWQWPAMALVFALVIAAMVIDYLGVPDIYR"
     gene            complement(4566..4868)
                     /locus_tag="PLB86_07790"
     CDS             complement(4566..4868)
                     /locus_tag="PLB86_07790"
                     /inference="COORDINATES: protein motif:HMM:NF018487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HRC65077.1"
                     /translation="MSESQDPTDKKPVVVPPAAKVAQARQAAEAEKRRQAKALDAPNP
                     SWWAPVFVTLLLAGLLWIVVFYISQGLWPVAAFNYWNLVVGFALLIAGFAMTMKWK"
     gene            4978..5718
                     /locus_tag="PLB86_07795"
     CDS             4978..5718
                     /locus_tag="PLB86_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HRC65078.1"
                     /translation="MPPASPRSRPGYWRLLAPAVFALAGLLFATSFQAARGTDLRSDR
                     GLPGLIDGANRDVAAKSAAITTLQTELDALTAAAVPSTTGLAPITDVINLLAPVVGTR
                     AVQGPAVTVALTDSKRNSANLPASVRPDDLVVHQQDVQAVVNALWRGGAEAMMIQDQR
                     IIATSAVRCVGNTLILQGRVYSPPFVISAIGRIDALNEALDQDPQIAIYKEWVDAVGL
                     GYRVTTEAKATFPAYSGPVSQLVAKVAR"
     gene            5715..6437
                     /locus_tag="PLB86_07800"
     CDS             5715..6437
                     /locus_tag="PLB86_07800"
                     /inference="COORDINATES: protein motif:HMM:NF033747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HRC65079.1"
                     /translation="MRVFRATLGVIGELLVTAGVVVLLFVVWQVGYSSLIEGRAQAGV
                     VAELEALGPDGTSLPELPEGPLVGGVAPTLDDGSIMAIIRIPRFGTDWARPVYEGITP
                     GTLAKGPGHYPRSSMPGQIGNMAIAGHRTTHGHPFFDLDALVEGDVVIIETRQSYVVY
                     RMARSTVVRPSQSEVVAPVPQKLGAKPTEAWLTLTTCHPKFSAAQRLIVFAKLDRVVP
                     RSEGAPPEMTTAPSALSLVGGA"
     gene            6439..6627
                     /locus_tag="PLB86_07805"
     CDS             6439..6627
                     /locus_tag="PLB86_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65080.1"
                     /translation="MYAALWRALPGRRGAKLATMILLFLIVVAVLFTWVFPAVAPLLP
                     FDQVTIDGAWAPKGVSGV"
     gene            6590..7252
                     /locus_tag="PLB86_07810"
     CDS             6590..7252
                     /locus_tag="PLB86_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRC65081.1"
                     /translation="MEPGLRKACLVSERPTILVVDNYDSFVFTIVGYLEQLGAECTVV
                     RNDAVTPAEGAGYDGVLVSPGPGTPEDAGVSMAMIAACAERSQPMMGVCLGHQALGVV
                     MGATVGRAPELLHGKTSQVEHDGTGVLAGLPQPFTATRYHSLAIDPATLPAELLANGH
                     TASGLVMAVRHASLPLHGVQFHPESVLTEGGHRILANWLAMCGDSEAVARSAGLSPLV
                     HH"
     gene            complement(7346..9235)
                     /gene="pknB"
                     /locus_tag="PLB86_07815"
     CDS             complement(7346..9235)
                     /gene="pknB"
                     /locus_tag="PLB86_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HRC65082.1"
                     /translation="MNTRRLLGGRYEVGEVIGRGGMADVHAGFDTRLSRQVAIKLLRS
                     DLARDAVFLARFRREAQSAAGLNHPNIVAIFDSGEDDSTDPTTGVAVHVPYIVMEVVD
                     GRTLREHLTATGPMPAAEAGRIVSDVLAALDYSHSKGIVHRDIKPANVMLGRGGAIKV
                     MDFGIARALADSAATMTQTQSVVGTAQYFSPEQAQGKPVDARSDLYSTGCMLFELLTG
                     RTPFVGETAVAIAYQHVGEPATPPSSIRSAVPAAYDAITLHAMVKDRDARYQTALEFR
                     DDLLAAGEGRAISAAARGTAANGTGGFGVGAGGAAVGAVGDAAATTVLSEVGGPGGGS
                     GMPPQDLRYDDDYDDEPLTRAGRRASQDPPKRRGAAYVLLTLAALAALALLFVLGRSI
                     LAQNAKPDQVAVPYLINQTEEQARALLSARKLLIDARNVTNDTVAKGLVVDQDPDSET
                     MVSIGSTVTVQVSSGPGQAAIPSLAGLTKDAAVTALKILNFPTPGVVVLDDPSQAKDA
                     VIGTDPPAGTVVPLTQQITLKVASGKVAVPSLVDQNQVTAQATLAGLKLLYAVAERVP
                     TADTTILEGTVLSQDIVPGTLVDVGSTVNVKIAIRLISTVTVTAPQPPPPPSTSSTAT
                     STTGG"
     gene            complement(9257..>10294)
                     /locus_tag="PLB86_07820"
     CDS             complement(9257..>10294)
                     /locus_tag="PLB86_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRC65083.1"
                     /translation="ANVMVTPEGKVKLTDFGIARAVDAVGHTRTGEVMGTPQYLAPEQ
                     AMGQRVTGATDLYSLGVVGHEMLTGKRPFDAGSAVATALAQISEPPPPLPPHVTDPLR
                     SAIEACLAKDPAQRPSSAASLAALLGMPVSGVAHASLATSVIPLSDLGATQPLATPTP
                     LPTQVYAHPVAPYGHYPATAAGPAYPPQPQPPDRRRGMGWWWLPVIAAFLVAGLFIWQ
                     NLVVGRANTPIPSVTVTRTVPRTSTASTPATTSAAPTTSTAPTTTPVKTVTLVESDYV
                     GQTTGVVRADLTTLGFLDIATKSVPSDRPKGEVLGISPTGPVPVTTTIVITVSAGAAP
                     TASSTAGGTSG"
BASE COUNT         1450 a         3583 c         3504 g         1657 t
ORIGIN      
        1 ttcgccttcg acaaggtcgc cttcgaggcc atctcggcct tcgccaccgt ggggctatcg
       61 accgggatca cccccacgct gcccggtatc ggacaactca tcctcatggc cctgatgtat
      121 gtgggccgag tgggcacggt gaccacagcg gtcgccttgg ccttgaacac tcggcacagg
      181 cattaccggc tccccgaaga aaggccgatc attggttagc aacccgttcc gcagggacga
      241 cgagaaccct cccgtgatca tcatcggcct gggtcggttc ggcgtctccg tcgctcgctc
      301 actcgtcgcg atgggacagg aggtcatggc cgtcgacctc gacgaggtcc gcgtgcagcg
      361 ctacgccgac gagttcaccc acgtcgtcca ggcagacagc tccgaccgcg acgccctgga
      421 acaactcggg gtcgagtctt tcgacaaggc cgtcatcggc atttccagca acgtggagtc
      481 cagtgtcatg acggccctgg ccctggtcga ctatgagata ccggacatct gggccaaggc
      541 gagcaccaag acccagggcc ggatccttga gcggctcggc gtccaccacg tcgtctatcc
      601 cgaacgcgcg atgggtgagc aggtggccca catgatcgtc ggagggatga cgcagtacct
      661 cgagttcgac gacgacttcg ccatcgctcg caccatggcc taccacgagc aatggggcaa
      721 gacgttcgcc gagaccggcg tccgcgcgaa gtatggcgtg acgatcgtcg gggtcaagcg
      781 gatcggtgag gacttcatct acgcccgtcc cgagaccctc gtccagcctc aggacgagtt
      841 ggtcgtgtcc ggccccacca agaacgtcga gcacttctgc gccttgagca agccgaagat
      901 cgcccgccag ccgcactgac acgcctcacc gcgtgtgagt tccgtcgccg tatcggtgtt
      961 tcggtgttga aaccaggacc cttggtcctt ttcccacgcg cgccggagtt actcttgagt
     1021 agtgggcata gctctagctc agcagccgca acgaggcgac ggaatgacca gcccggtaga
     1081 cgttctcacc gaggtggagt gttgggcggt cctgcgccgt caggagctcg gtcggctcgc
     1141 cttccgtcac ggcgacggac tccagatcac cccggtcaac ttcgccgtcg cgggcgacca
     1201 cgtggtcttc cgcaccggtg acggaggcaa gttcagcgat ctcgtgcaaa acccggaggt
     1261 cgccttcgag gtcgacgcca cggtcggcga ggtcgccgag agcgtcgtat gccgcggggt
     1321 cgtcgtcgaa ttgaccggcg agcaggccct gatgaccgac caactgcgac tgcgtccctg
     1381 ggtcgcctcg cacaagaccc acgtcatggc gatccgggtc agcgagatca ctgggcggcg
     1441 cttccatctc accaagccct ggtcgcacat ccgcccagtc tgagcctgcc gggtgacccc
     1501 cggcctgaac gtcctgcgcc ccgcgcggtg gtccggcggg gcacaggggt gtccggggtc
     1561 aactcgaccc ggcgaccgac atcaggcgtc ggtgtcggcc ttgtcatcgt cggcaacacc
     1621 gtccacagcc acagcctccg cagccacatc gtccnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnntcgggc tggtccgcaa ccggggcgac ggtcgacgcg cgtccggtcg
     1801 tcggcggcag gtatgccgtc tccgtcaccg gggcggccca cgggtcgtcc ttgggcttgc
     1861 gggacatgat caccgcaatc gcggcagcgg cagcggcgcc gatgccgaac agcagcagca
     1921 acccgccctt cttgctcttg cccttggcct tgcccgccga cccggccacg gccggcgcgt
     1981 ccgtgctgct caccgcatcg ctgaccgctt ccgtcgccgc ggcgaacgcc tgcggagcac
     2041 cggcagccag gtcgcgcttg gccgagcctg ccgcggcggc aacggcggcg atggcggccg
     2101 acaccttggg caggaggtcc tcgacgaggc tgtcgcgggc ctgatccacc ttgggggcga
     2161 tttcatcgac cgcggcctgc agccgtgggg cggcgacctc gactccatgc tcgacgcgcg
     2221 gccgagccca ctcccgcccg gcctccgcgg cttccttggc ccgctcggca tacggcgccg
     2281 ccgcttcgcg agcagcccct gcggcttcct tggctttctc ggcatacggg gcggccgctt
     2341 cacgggcggc ctccgcggcg tcgcgggccc gaccggcata tggtgcggcc gcctcacgcg
     2401 cgcgaccggc atacggtgcg gccgcctcac gcgcgcgacc ggcatacggt gcggctgcct
     2461 cacgcgcggc atcggcggcg tcgcgcgccc gaccggcata cggggccgtg gcgtccttgg
     2521 cccgctcggc caagggagta gccacctcgc gagcccggtc ggcgaggggg gcagcggcct
     2581 gcatcgctcg ggtgcgcccg cgctgggccg ccggtgccac cgactccctc agctgggcca
     2641 gcgcaccgaa gacggtgcca tcaccgcgct cgccgcgggc cgcggccagc cgcccaaaca
     2701 ccgacaggtc ctcggtcttg tcactcgcac gcttccggga cactgatcct cctggtggtt
     2761 cgtggtggtc gtccatccaa gcacctggac ccctcgcggc gtcgtcgcct catcgtcgtt
     2821 gtcgccgaca ctgtcgtcaa cgcccgccgc tgccggggtc agtggtcaca tcctgccctg
     2881 tcccgggtcc agacacacac ccgcctcccc cgaacgcacg ctggagcgcc gtctgggaca
     2941 cccggcatac cacggggtat gccgtgcgag gatgttcgcc atgaaggcaa ccctccacac
     3001 caaccacggc gacatcgtcg tcaccctctt cccgaacgag gcgcccaaga cggtggccaa
     3061 cttcgtgggg ctcgcgacgg gcgagaagga ctaccgcgac gacgccaagc ggacgaaccc
     3121 gaccaagttc tacgacggtc tggtcttcca ccggatcatt tccggcttca tgatccaggg
     3181 cggctgcccg ttgggtcagg ggtatggcgg gccgggcttc accttcgacg acgagccgca
     3241 ccccgacaag accttcgacc gcccctacct gctggccatg gccaacgccg ggaagcggat
     3301 gggcaagggc accaacggct cgcagttctt catcaccgtc tcgacgccgg gttacctcaa
     3361 cttcaagcac acgatcttcg gcgaggtggc cgacgccccg agccgtgcga tcgtcgacgc
     3421 gatcgccgcc gtgcgcaccg accgcaacga ccgcccgctt gagccggtcg tcatcgagtc
     3481 ggtcaccgtc gaccaggact gagccacccg cagtgggcag gcgcgcgacc gaggtgagcg
     3541 ggcggcatac atgagctatc ccaccctgcc tgagggcgtc ggtggggcga ccccccgccc
     3601 ggtctgcccc cgccaccccg accgagagtc gtgggtgagt tgtcaacggt gtcgtcggcc
     3661 ggtgtgcccg gagtgccaac ggccggcgcc cgtgggcgtg caatgcgtgg attgtgtgcg
     3721 cgagtcggcc cgcacggtcc gtccgacgcg caccgccttc ggtgggcgca ccatcgacgg
     3781 caaccccctg gccaccaagg cgatcatcgc catctgcgcc gcggtctatg tcttgcagtt
     3841 cctgcgcccg gtgacgaccg aggacctcgc cttcgtcccc gtcatcgggt ggcacgagcc
     3901 gtggcgcgcc ctcaccggcg cgttcctgca cagcccgtcc acggtgttgc acctcgggct
     3961 caacatgctc atgctctggc agatcgggcc ctatctcgag agcctgctcg gccgggcccg
     4021 cttcctggcg ctctacctcg tctcggccgt cggcgggtcg gccggcgtgc tgctgctggc
     4081 ccccgcgccg cgcagcatcc tcttcacctc ggccgaggcc gcgcagtatg ccgcctgggc
     4141 cactcccgtc gtcggcgcct ccggggcggt cttcgggctg ttcggggcgc tcctcgtgct
     4201 caaccggcat ctcggtcgat cctcgacgcc gctgtatgcc gtgctggccg tcaacatcgt
     4261 cttcgggttc atctatcccg ggatcgcctg gcaggcccac ctgggtggcc tcgtcacggg
     4321 caccgccgtc gccggggtgt tcgccgtgct ggcccatcgg gatcggcgct cgtggcagtg
     4381 gccggcgatg gccctggtgt ttgctctggt catcgccgcg atggtcatcg attacctcgg
     4441 cgtgcccgac atctaccgct gagcggcccc ggcggagcgg ccggcgaagt tatccacagg
     4501 cttgtccaca gctggggaga actacacgga tgtaactcgc cggacacccg atgagtgggc
     4561 cgaggtcact tccacttcat cgtcatggcg aacccggcga tgagcagcgc gaagccgacg
     4621 acgaggttcc agtagttgaa cgccgcaacc ggccagaggc cctgcgagat gtagaagacg
     4681 acgatccaca gcagccccgc aagcaggagc gtgacgaaga ccggcgccca ccacgacggg
     4741 ttgggcgcat cgagggcctt agcctggcgg cgcttctcgg cctcggccgc ctggcgcgcc
     4801 tgggcaacct tggctgccgg cgggacaacg accggcttct tgtcggtggg gtcctgcgac
     4861 tcggacacga ggtctccttg cgttcttgct ggtgtgcctt agcgtaggtg atggagcacg
     4921 cactcgcgtg ccgctcgctc gcgcgcgggc tcgactagtg ctgcaggagg tgtcggtatg
     4981 ccgcccgcct cccctcgatc ccgtccaggc tactggcggc tgctggcccc ggcggtgttc
     5041 gcgctggccg gcctgctctt cgccaccagc ttccaggccg cccgtggcac cgatctgcgc
     5101 agcgaccgtg gcttgcctgg tctcattgac ggggccaacc gcgacgtggc cgccaagtcg
     5161 gcggccatca cgaccctgca gaccgagctc gacgccctca ccgccgccgc ggtgccctcg
     5221 acgaccggcc tggccccgat caccgacgtc atcaacctcc tcgcaccggt cgtcggcacc
     5281 cgagccgtgc aggggccggc ggttacggtg gcgctcaccg actccaagcg caactcggcc
     5341 aacctgccgg catcggtgcg acccgatgac ctcgtcgtcc accagcagga cgtccaagcg
     5401 gtcgtcaacg cgctgtggcg gggcggggcc gaggcgatga tgatccagga ccaacggatc
     5461 atcgcgacct cggccgttcg gtgcgtgggc aacacgttga tcctgcaggg ccgggtctat
     5521 tcgccgccgt tcgtcatctc ggcgatcggg cgcatcgacg ccctcaacga ggcgctcgac
     5581 caggacccgc agatcgccat ctacaaggag tgggtcgacg cggtcggtct cggctatcgg
     5641 gtgacgaccg aggcgaaggc gaccttcccg gcatactcag gtcccgtgtc ccaactggtg
     5701 gcgaaggtgg cgaggtgagg gtcttccgcg caacgctcgg cgtgatcggc gaactgctgg
     5761 tcaccgcggg cgtcgtcgtg ctgctcttcg tcgtctggca ggtgggctac agcagcctca
     5821 tcgaaggccg ggcgcaagcg ggggtcgtcg cggagttgga ggcgttaggg ccagacggca
     5881 cgagcctccc cgagcttccg gaaggccccc tggtcggggg cgttgcgccc acgcttgatg
     5941 acggctcaat catggcgatc atccgaatcc cgcggttcgg caccgactgg gccaggccgg
     6001 tctacgaagg gatcaccccc ggcaccctgg cgaagggacc gggccactac ccacggtcat
     6061 cgatgcccgg ccagatcggc aacatggcga tcgctggcca ccgcaccact cacggacacc
     6121 cgttcttcga ccttgatgcc ctcgtcgaag gtgatgtcgt catcatcgag acccgccagt
     6181 cctacgtcgt ctatcggatg gctcgctcga cggtcgtccg gccgagccag tccgaggtcg
     6241 ttgctccggt gccgcagaag ctgggcgcca agcccaccga ggcctggctg acgctgacca
     6301 cgtgccaccc gaagttctcc gcggcccagc gcctcatcgt cttcgccaag ctcgaccggg
     6361 tcgtcccccg cagcgaaggc gccccgccgg agatgacgac cgccccgtcc gccctctcgc
     6421 tcgtcggagg tgcctgagat gtatgccgcc ctctggcgag cgctccccgg caggcgcggg
     6481 gccaagctgg ccacgatgat cctgctcttc ctcatcgtcg tcgccgtgct cttcacctgg
     6541 gtcttccccg cggtcgcgcc cctgttgccc ttcgaccagg tgaccatcga tggagcctgg
     6601 gctccgaaag gcgtgtctgg tgtctgagcg ccccacgatc ctcgttgtcg acaactacga
     6661 ctcgttcgtc ttcaccatcg tcggctacct cgagcaactc ggcgccgagt gcacggtggt
     6721 ccgcaacgac gcggtgaccc ctgccgaagg cgccggctac gacggggtgc tcgtctcacc
     6781 gggccccggc acccctgagg acgccggcgt gtcgatggcg atgatcgcgg cctgcgccga
     6841 gcgctcgcag ccgatgatgg gcgtctgcct gggccatcag gcactcggcg tggtcatggg
     6901 ggcgacggtc ggccgggctc cagagttgct gcatggcaag acctcgcagg tcgagcatga
     6961 cggcacgggc gtcctcgccg ggctgccgca gccgttcacc gccacccgct atcactcgct
     7021 ggcgatcgat ccggccaccc tcccggccga gctactagcc aacggccaca ccgccagcgg
     7081 cctggtcatg gcagttcgcc acgccagcct tccgctgcac ggcgtgcagt tccaccccga
     7141 gagcgtgctc accgagggcg gccaccggat cctcgccaac tggttggcga tgtgtggtga
     7201 ctccgaggcc gtcgccagat cggccggcct cagcccgctc gtccaccact gacgcccgcc
     7261 actgacgtcc gccggcgggc cgtcagccac tggcgtccgc cggccggctg tcagcctccg
     7321 cgtacgctcc ggcccctacc tccggctacc ctcccgtggt cgacgtcgcc gtcgatgagg
     7381 tggacggcgg gggcggcggt tggggtgccg tgaccgtgac cgtcgagatg agccggatgg
     7441 cgatcttgac attgaccgtc gagccgacgt cgaccaacgt tccggggacg atgtcctggc
     7501 tgagcaccgt gccctcaagg atcgtcgtgt ctgctgtcgg cacccgctcg gcgaccgcat
     7561 agaggagttt gagtccggcg agcgtggcct gggccgtcac ctggttctgg tcgacgaggc
     7621 tgggcaccgc gaccttgccc gaggccacct tgagggtgat ctgttgggtc aacgggacga
     7681 ccgtgccggc cggcggatcg gtgccgatga cggcgtcctt ggcctgcgac gggtcgtcga
     7741 gcacgacgac gccgggtgtt gggaagttga ggatcttcag ggcggtgact gccgcgtctt
     7801 tggtcaaccc agcgagcgag gggatcgccg cctgacctgg cccggacgac acttggacgg
     7861 tcaccgtgct cccgatgctg accatggtct cggagtcggg gtcctggtcg acgacgagtc
     7921 ccttggcgac tgtgtcgttg gtgacgttac gggcgtcgat gaggagtttg cgcgccgaca
     7981 gcaatgcccg ggcttgctcc tcggtctggt tgatgaggta gggcactgcc acctggtccg
     8041 gcttggcgtt ctgggccagg atgctgcggc cgaggacgaa cagcagggcc agagcggcca
     8101 gtgccgcgag ggtgagcagc acgtatgccg ccccgcgtcg cttgggcggg tcctgcgacg
     8161 cccgacgtcc cgcgcgagtg aggggctcgt cgtcatagtc gtcgtcgtaa cggaggtcct
     8221 gcggcggcat accggatccg ccgcctggac cacccacctc ggagaggacc gtcgtggcgg
     8281 ccgcgtcgcc gaccgcgccg accgctgcgc cccccgcgcc cacgccgaac ccacccgtgc
     8341 cgttcgcagc ggtgccgcgg gcggcggcac tgattgcccg gccctccccc gcggcgagca
     8401 ggtcgtcgcg gaactccaac gcggtctggt agcgcgcgtc gcggtccttc accatggcat
     8461 gcaaggtgat cgcgtcgtat gccgcgggca ccgccgagcg gatcgacgac ggcggcgtgg
     8521 ctggctcgcc gacgtgctgg taggcgatcg cgaccgcagt ctcaccgacg aacggcgtgc
     8581 gcccggtgag cagttcgaag agcatgcacc cggtcgagta gaggtcggag cgggcgtcga
     8641 ccggcttgcc ctgggcctgc tcgggcgaga agtactgcgc ggtgccgacg accgactgag
     8701 tctgggtcat cgtcgctgcg ctgtcggcga gggctcgggc gatcccgaag tccatgacct
     8761 tgatcgcgcc gccgcgaccg agcatgacgt tggcgggctt gatgtcccgg tggacgatgc
     8821 ccttggagtg gctgtagtcc aacgcggcga ggacgtccga cacgatgcgt ccggcctcgg
     8881 cggcgggcat cgggccggtg gcagtcaggt gctcacgcag ggtgcggccg tcgacgacct
     8941 ccatgacgat gtaggggacg tgcaccgcga ccccggtcgt gggatcggtg ctgtcgtcct
     9001 cccccgagtc gaagatggcg acgatgttgg ggtggttgag cccggccgcc gactgcgcct
     9061 cacggcggaa gcgggcgagg aagacggcat cgcgcgccag gtcggagcgc agcagtttga
     9121 tcgccacctg tcgggacagg cgcgtgtcga agcccgcgtg gacatcggcc atgccgccac
     9181 ggccgatgac ctcgcccact tcgtaacgcc ccccgagtag gcgtcgagtg ttcatgcgga
     9241 tcccccgtgc agtgcgctag ccactggtgc ctcctgcggt gctgctggct gtcggtgccg
     9301 ctcccgcgga caccgtgatg acgatggtgg tggtgaccgg caccgggccg gtcggactga
     9361 tcccgaggac ctcgcccttg ggacggtcgg agggaacgga cttcgtggcg atgtccagga
     9421 agcccaaggt ggtgaggtcg gcccgcacga ctcccgtcgt ctgaccgacg tagtcggact
     9481 cgacgagggt caccgtcttg accggagtgg tcgtcggggc ggtgctggtc gtcggggcgg
     9541 cgctggtcgt cgcgggcgtg ctggccgtgc tcgtccgggg gacggtcctc gtcaccgtca
     9601 cggaggggat cggcgtgttg gcccggccga cgacgaggtt ctgccagatg aacaggccgg
     9661 cgacgaggaa ggcggcgatc accggcagcc accaccagcc catcccgcgc cggcggtcgg
     9721 gtggctgcgg ctgcggcggg taggccggcc ccgctgccgt cgctggatag tgcccgtagg
     9781 gcgccaccgg atgggcgtag acctgggtcg gcagcggagt gggagtggcc aacggctggg
     9841 tggcgcccag gtctgacaac gggatgacgc tggtcgccag gcttgcgtgc gcgaccccgc
     9901 tgaccggcat accgagcagg gcggcgagcg aggcggcgct ggaggggcgt tgggcagggt
     9961 ccttggcgag gcaggcctcg atggccgacc gcagcgggtc ggtgacgtgc ggcggcagcg
    10021 gcgggggcgg ctcgctgatc tgcgcgaggg cggtggcgac ggccgacccg gcgtcgaagg
    10081 ggcgcttgcc cgtgagcatt tcgtggccga cgaccccgag ggagtagagg tcggtggcgc
    10141 cggtcacccg ttgacccatg gcctgctcgg gcgcgaggta ttgcggcgtg cccatgacct
    10201 cgccggtgcg ggtgtgcccc acggcgtcga cggcgcgcgc gatgccgaag tcggtgagct
    10261 tgaccttgcc ctcgggcgtg accatgacgt tggc
//
LOCUS       DAORPW010000080        10169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1515_length_10169_cov_813.114198, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000080 DAORPW010000000
VERSION     DAORPW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1515_length_10169_cov_813.114198"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLB86_07825"
     CDS             <1..719
                     /locus_tag="PLB86_07825"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRC65084.1"
                     /translation="AGGVDPSGQRTYHLLVRQHQLGLLRQGPIRRTGAGPASFEPTFV
                     LAEGEADGAAGGCGSGSVRRPERAEALAILADRYVASHGPVTERDFAGWCDQPLRLVR
                     EAVAATEGRVRVETVGEAAYLVHEDAPPAASEQTSPVLLLPGFDEWLLGYKDRSAQLT
                     REQERAVVPGGNGVFRGTLVADSAVVGTWRRAVARGTITVEVTPFTRPSRALRSGADE
                     AAAAYGRFWELPCTLRWVDG"
     gene            712..1737
                     /locus_tag="PLB86_07830"
     CDS             712..1737
                     /locus_tag="PLB86_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRC65085.1"
                     /translation="MARGGIPPSPLPQREGVDAVRLRMPQGGPWALLRDHLIERIPLP
                     PHQIDAMLAAGEFVGPDGMPLAPDAPFVPRSVVWVHRDLPDEVPVPFKVTVLHHDERI
                     VVVDKPPFLATMPRGRHIRQTVLARLRVELGLPRLAPAHRLDRLTSGVLVLTTERRWR
                     GPYQQVFATGGADKDYLACAPLPKGGALPSGVVLPATVEVHLSKPHGQLQSHVLADRI
                     PNSRTDIDLIGRDEEAGVGLYHLRPHSGRTHQLRAHLAWLGIPICGDPLYPEIRDVDP
                     DDFQTPLGLVARRLRFRDPVDGTPRAFESDRVPWPWSGAAPTLAVAPVIDGSIPEVAS
                     PSGETKE"
     assembly_gap    2095..2194
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2195..3934
                     /locus_tag="PLB86_07835"
     CDS             <2195..3934
                     /locus_tag="PLB86_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRC65086.1"
                     /translation="LDENVEAGDADFFAVGASEMSPDGRLLAWSADREGDERYDLVVR
                     EVDSGAILDAAVRGIGDPLAWSLDNRHIFYTRLDDSWRSHEIWRHEVGTAVEADELVL
                     AESDERFFLSVGATADDRWIVLSATSKTAAQVWLIDAADPRQAPRSVLPRRDEVIYDV
                     DPLADGLLVLHNAGRANFQVDWLPSAGAPVEDLVDLGWTTPDEFVTGMDAFATFVVLS
                     LRVDGLTALRFVPVTGEGPGGFGMPHDLTFPGETGTLTLGSTPDPTSPTVQVAHTSMA
                     TPPAIYDYDVRARTLSLLKAHPVPGVDLSALRETRIWATAPDGVAVPVSLVHHADVRP
                     DGTAAVLLYGYGAYGIPSDPWFSVARLSLLHRGVVFAIAHVRGGTELGWDWYVQGRLE
                     HKPHSFTDFLACADALVDQGWAAADRVAAEGGSAGGLLVAAAATQAPARFRAVLAQVP
                     FVDVLTTMLDPSLPLTVTEQQEWGDPIADPAAYDLIASYSPYETVPVGDFPALLITTS
                     ANDTRVFVTEPAKWMARLRDRVTDDPVCRPLLMRTQLTSGHGGRSGRYQAWREIAWEW
                     AVLLDLLGAADQG"
     gene            complement(3925..4707)
                     /locus_tag="PLB86_07840"
     CDS             complement(3925..4707)
                     /locus_tag="PLB86_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC65087.1"
                     /translation="MRIPTQGLVSVDTTADLVRLGLVLAVLVALAAAVSRLAGLGYSR
                     AQVTAALRATLQLALVGSVIAAVLDSWWLTAGFAALMLAVASVTAGRRVAPPGSRGWV
                     WGFVPICAGALPTAAILLATGVVPLVPIAVVPVVGILVGGAMTATTLSGRRVADALRS
                     RHGEVEAALSLGLLDRDARLLVSRSDAGLALVPGLDQTRTVGLVTLPGAFVGMLLGGA
                     TPLQAAAVQLVVLVALLLVAVVATAITLELSARGTFARPTQP"
     gene            4789..6153
                     /gene="hisD"
                     /locus_tag="PLB86_07845"
     CDS             4789..6153
                     /gene="hisD"
                     /locus_tag="PLB86_07845"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRC65088.1"
                     /translation="MNRTDLRGSRLSRRTLREVLPRAEFDVAAAAEAVRPVVQDVAHR
                     GAAALRELSERFDGVVPPQLRVPAEVLEAAVAQLDPALRAALEESIRRARIVHHDQRR
                     ADVTTRVVEGGTVTERWVPVERVGLYVPGGLAVYPSSVVMNVVPAQAAGVGSLAVTSP
                     PQRENVGVFTGYPNPTILATCALLGVDEVYAAGGAQAIAMLAYGFDETVTHADGSTET
                     LVCEPVSLITGPGSIWVTAAKRLVQGLVGIDAEAGPTEIAVLADDSADPEHVAADLLS
                     QAEHDPLAASVLVTVSEALADAVDAALARRVPDAKHGERIREALTGRQSRVVLVDDLD
                     AGILVCDVYAAEHLEIQTRDAAEVAGRIHNAGAIFVGPWSPVSLGDYAAGSNHVLPTA
                     GSAVHSSGLSVQSFLRGIHVVAYDEAALRGVAHHVVTLAQAEDLPGHGQAVTARFGDA
                     VPTP"
     gene            6150..7343
                     /locus_tag="PLB86_07850"
     CDS             6150..7343
                     /locus_tag="PLB86_07850"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRC65089.1"
                     /translation="MSAPAVPTDVAQRVARLLRDDLRGQTAYGAPQLDVPVRLNTNEN
                     SYAVPPDVVAAITAAVAGCASDLNRYPDREFTALREDLAAWLTEQTGVAVEAAQVWAG
                     NGSNEVLQHVVQAFAGPGRVALGFTPAYSMHPIISRTMGSTWVDGHRGGPDGPFDLTE
                     GDVVAQVLAAAPHVVFLCSPNNPTGTALDLEIVRAVLDAAPDAVVVVDEAYAEFARPG
                     TPSALSLLAAYPQLVVTRTMSKAFALAGGRLGYLVAAPEVIDALRLVRMPYHLSTPTQ
                     EIARAALAHRLSLLAEVEAIKNQRDRIVETLAAQGFPPVPSDANFVLFGGLADAGRTW
                     EALLAHGVLVRDVGIAHHLRVTAGTPAETTAFLDAMAALPAEHRSQTAPEPSAEPSAS
                     QGAPA"
     gene            7340..7957
                     /gene="hisB"
                     /locus_tag="PLB86_07855"
     CDS             7340..7957
                     /gene="hisB"
                     /locus_tag="PLB86_07855"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRC65090.1"
                     /translation="MSDQPARRATVRRATSESSVELTVDLDGTGRSTVSTGVGFYDHM
                     LASLAKHALLDLDVAAVGDVDIDAHHTVEDVAICLGDALREALGDKRGISRFGDATVP
                     LDEALVQAVVDVAGRPYCVHTGEPAGQEFVVIGGSYIGSLTRHVFETLAHHAGIALHV
                     RVLAGRDPHHIVEAQFKAVARALRAAVAPDPRVHGIPSAKGTLSR"
     gene            7954..8619
                     /locus_tag="PLB86_07860"
     CDS             7954..8619
                     /locus_tag="PLB86_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65091.1"
                     /translation="MSPDAGLGDGAASGAGSSGGGLLLVRAGVSTVREWALDGLGPVI
                     VVPDPSGWTILVPDGDRTFAAPPFDDGVGALLGRPVRRRLLPTIGVALVGNRLAISVV
                     PAVLRPGRSWLVWEPGAGLVRPAGLTPASVAQLAAAADRREHRDLVAAMMADGRGSAA
                     RVLRDVFEALSLPGAEFATGERVPVDEPGAVRIEPSREDVARFDRTVHEVRRWREEVE
                     GSP"
     gene            8616..9254
                     /gene="hisH"
                     /locus_tag="PLB86_07865"
     CDS             8616..9254
                     /gene="hisH"
                     /locus_tag="PLB86_07865"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRC65092.1"
                     /translation="MSPTVVVFDYGSGNVRSAVRSLERVGAEVTLTAHADEALAADGL
                     VVPGVGNFHACMAGLRAADGARIIEVRLAGNRPVLGVCVGMQVLFDGSTEPAAHEEPG
                     LGEWPGMVERLVAPVVPHMGWSPVQVPDGSRLFAGVESERFYFVHSYAVQSWTLEPHG
                     PFAPRAPMVTWSEHGAPFVAAVENGPLAATQFHPEKSGDAGAHLLENWVRSL"
     gene            9251..9589
                     /locus_tag="PLB86_07870"
     CDS             9251..9589
                     /locus_tag="PLB86_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65093.1"
                     /translation="MNAKRASNTRRTTTTRRRRSTTTSSGYRRRRRPGLSSTVGSAIG
                     MLAVTTLLNLSWPARIGLLVLVLVVGLAYMLWRHRAEIAAGADGIPEDAAAPPTPQDR
                     PTPSPEENPS"
     gene            9586..>10169
                     /locus_tag="PLB86_07875"
     CDS             9586..>10169
                     /locus_tag="PLB86_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRC65094.1"
                     /translation="MTESPRLQLLPAVDISDGGAVQLQQGVAGSGWKFGDPWLAAKRW
                     QDDGAEWIHLVDLDAAFGRGNNRELIADIVGRLDLQVELSGGIRDADSLAVALATGCR
                     RVNVGTAALENPEWTARAIAEHGDRVAVGLDVRGTRLAARGWTSEGGDVWETLDRLDA
                     EGCSRYVVTDVTKDGMLAGPNLALLREVAARTDRP"
BASE COUNT         1370 a         3639 c         3577 g         1483 t
ORIGIN      
        1 ccgcgggtgg cgtcgacccg agcggccaac gcacctatca cctcctggtc cgtcagcatc
       61 aactcggcct gttgcgccaa gggccgatcc gccgcaccgg ggccggtcca gcctccttcg
      121 agcccacctt cgtgctcgcc gagggcgagg ccgacggggc tgcagggggc tgcggttccg
      181 gctccgtgcg ccgaccggag cgtgccgagg cgctcgccat cctcgccgat cgctatgtcg
      241 ccagccacgg cccggtcacc gagcgtgact tcgccggctg gtgcgaccaa cctctgcggc
      301 tcgtccggga ggccgtggcc gccaccgagg gacgggtgcg ggtggagacg gtgggggagg
      361 cggcatacct cgtccacgag gacgccccgc ccgcggcgag cgagcagacg tccccggtgc
      421 tgctgctgcc cgggttcgac gaatggttgc tcggttacaa ggaccgcagc gcccaactca
      481 cccgcgagca ggagcgcgcg gtcgtccctg ggggcaacgg cgtgttccga gggaccctgg
      541 tggcggacag tgcggtcgtc gggacctggc gacgtgccgt ggcccgcggc acgatcacgg
      601 tcgaggtgac gcccttcacc cggccctcgc gcgccctgcg gtcgggagcg gacgaggcgg
      661 cggcggcata cgggaggttc tgggagttgc cctgcacgct gcggtgggtc gatggctaga
      721 ggtgggatcc cgccgtcccc gctgccgcaa cgtgaggggg tcgacgcggt ccggttgcga
      781 atgccccagg gcgggccgtg ggctctgctt cgcgatcatc tgatcgagcg gatccccttg
      841 cctccccacc agatcgacgc gatgctcgcc gcgggggagt tcgtcggccc cgacggtatg
      901 ccgctcgccc cggacgcgcc gttcgtgccc cgatcggtgg tgtgggtcca ccgagacctg
      961 cccgacgagg tgccggtgcc gttcaaggtg acggtcctgc accacgacga gcggatcgtc
     1021 gtcgtcgaca aaccgccgtt cctggccacg atgccgcgcg gccgccatat ccgtcagacc
     1081 gtgctcgcgc ggctgcgggt cgagttgggg ttgccgaggt tggccccggc tcatcgcctc
     1141 gaccggctca cctcgggggt gctcgtcctc accaccgaac gacggtggcg gggtccctat
     1201 cagcaggtgt tcgccacggg cggcgcggac aaggactacc tcgcctgtgc gccgctgccc
     1261 aagggtggcg ccctgccgtc cggcgtcgtg cttcccgcaa cggtcgaggt ccacctcagc
     1321 aaaccgcacg ggcaactgca atcgcacgtc ctggcggatc gaatccccaa cagccgcacc
     1381 gatatcgacc tcatcggccg agacgaggag gcgggcgtgg gcctctacca cctgcgccca
     1441 cactcgggcc ggacccacca acttcgcgca cacctcgcgt ggctggggat cccgatctgc
     1501 ggcgacccgc tctaccccga gatccgcgat gtcgatccgg acgacttcca gaccccgttg
     1561 ggcctcgttg cccggcgcct gcgcttccga gacccggtcg atggaacacc ccgggcgttc
     1621 gagtccgacc gggtgccgtg gccgtggagc ggagcggcgc cgaccctcgc tgtcgcacct
     1681 gtgattgatg gatcaattcc tgaagtggct tcaccatcgg gtgagactaa ggaataatcc
     1741 cctccgggag gtatgaacct gggggctttc gggggtttac ctgtttgcca tggttcaaag
     1801 gggcttcctc gccgtttcgt cgattcgcct gtcgtgggga gacgacgcgt gggtcggtgc
     1861 ggtggagggg gaatagccgc ggtgggtggg tcgtgttgac acggcatacc cgccgcggtt
     1921 ccccctgtcc gacgctcact agagtgaccg gatgcaaccg ccccgtgccc cccgcgagcc
     1981 gcagtcgcgc atcgtgcacg ggcatgcggt cctcgacgac tacgcgtgga tggccgaccg
     2041 cgacgatccc cggctgcgcg cctacctcga agccgagaac gcgtatgccg cggannnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctcgat gagaacgtcg aggccggcga
     2221 cgccgacttc ttcgccgtcg gtgcgagcga gatgagcccg gacgggcgac tgctcgcctg
     2281 gtcggccgat cgtgaagggg acgagcgcta cgacctggtc gtgcgggagg tcgattccgg
     2341 cgcgatcctc gatgccgccg tccgggggat cggcgacccg ctggcctggt cgctggacaa
     2401 ccgccacatc ttctacacgc ggctggacga ctcctggcgc agccacgaga tctggcggca
     2461 cgaggtgggc acggcggtcg aggccgacga gttggtcctc gccgagtcgg acgagcgctt
     2521 cttcctcagc gtgggggcca cggccgacga tcggtggatc gtcttgagcg cgacctccaa
     2581 gacagccgcc caggtgtggc tcatcgatgc ggccgacccg aggcaggcgc cgcgcagcgt
     2641 cctgccccgg cgggacgagg tcatctacga cgtcgacccg ctcgccgacg gcctgctcgt
     2701 cctgcacaac gcgggccgcg ccaacttcca ggtggactgg ctgcccagcg ccggagcccc
     2761 ggtcgaggac ctcgtcgacc tcggctggac cacgcccgac gagttcgtca ccggcatgga
     2821 cgcgttcgcg accttcgtcg tcctcagcct gcgcgtcgac gggctcaccg ccttgcgctt
     2881 cgtgccggtg acgggcgagg ggccgggcgg tttcggtatg ccgcacgacc tcaccttccc
     2941 cggcgagacc ggcaccctca ccctgggctc gacgcctgac cccacgtcgc cgacggtcca
     3001 ggtcgcgcac acctccatgg ccacgccgcc ggcgatctac gactacgacg tgagggcccg
     3061 caccctgtcg ctgctcaagg cgcatccggt gccgggggtc gacctcagcg cacttcgtga
     3121 gacccggatc tgggccaccg cgcccgacgg ggtggcggtg ccggtgtcgc tcgtgcacca
     3181 cgccgacgtg cgccccgacg ggacggcggc cgtcctgctc tacggctacg gcgcctatgg
     3241 catcccgtcg gacccgtggt tctccgtcgc ccggctctcg ctgctgcacc gcggggtcgt
     3301 cttcgcgatc gcccacgtcc ggggtggcac cgagttgggc tgggactggt atgtccaggg
     3361 ccggctggag cacaagcccc acagcttcac cgacttcctc gcctgcgccg acgcgctggt
     3421 ggaccagggc tgggcggcag ccgatcgggt tgctgcagag ggaggttcgg ccggtggact
     3481 gttggtcgcc gccgcagcga cgcaggcccc cgcgcggttc cgggccgtgc tcgcccaggt
     3541 gcccttcgtc gacgtcctca cgacgatgct cgacccgagc ctgccgctga cggtcaccga
     3601 gcagcaggag tggggcgacc cgatcgccga ccccgcggca tacgacctca tcgcctccta
     3661 ctcgccctac gagaccgttc cggtcggcga cttcccggcg ctgctcatca cgacgagcgc
     3721 caacgacacc cgcgtgttcg tcaccgaacc cgccaagtgg atggcgcggc ttcgcgaccg
     3781 ggtgaccgac gatccggtat gccgcccgct cctcatgcgc acgcaactca cctcgggtca
     3841 cggggggcgt tcggggcgct atcaggcctg gcgtgagatc gcctgggagt gggcggtgct
     3901 gctcgatctg ttgggcgccg cagatcaggg ctgagtcggc ctggcgaagg tcccgcgggc
     3961 ggacagctcc aaggtgatcg ccgtggcgac gacggcgacg agcagcaggg ccacgaggac
     4021 gacgagttgg accgcggccg cctgcagggg cgttgcgccg ccgagcagca tcccgacgaa
     4081 ggcgcccggg agggtcacca acccgaccgt tcgggtctgg tccagaccgg ggacgagggc
     4141 gagcccggcg tcgctgcgag acaccagcag ccgggcgtcg cgatcgagca gtcccaagga
     4201 cagggccgcc tccacctcgc cgtgacgcga gcgcaacgcg tctgccaccc ggcggccgga
     4261 aagcgtggtc gccgtcatgg ccccgccgac gaggatgccg accaccggga cgacggcgat
     4321 cggcaccaga ggcaccaccc cggtcgcgag caggatcgcc gccgtgggca gggccccggc
     4381 gcagatgggc acgaagcccc agacccagcc gcgtgaccct gggggcgcga cccgacgccc
     4441 ggctgtcacc gacgcgacgg cgagcatgag ggcggcgaag ccggcggtca gccaccagga
     4501 gtcgaggacg gccgcgatga ccgagccgac gagggccaac tggagcgtcg cccgcagggc
     4561 agcggtcacc tgggctcgcg agtagcccag cccggccaat cggctcaccg cggcggcgag
     4621 cgcgacgagg acggccagga cgaggcccag ccggacgaga tcagccgtcg tgtcgaccga
     4681 aaccaggccc tgggtgggga tccgcatacg gcccatcatc gccggtggcc ggtcggctgt
     4741 ggacgggcca aggggaggtc ggtggacccg cctagacttt gccccgtgat gaaccggacc
     4801 gacctgcgtg gctcacgcct gtcgcggcgc accttgcgtg aggtgctccc gagggccgag
     4861 ttcgacgtgg ctgcagccgc cgaagcggtg cgccccgtcg tccaggacgt cgctcatcgt
     4921 ggggccgcgg cgctgcgcga gttgtctgag cggttcgacg gggtggtccc gccgcagttg
     4981 cgggtccccg ccgaggtgct cgaggccgcg gtcgcccaac tcgaccccgc cctgcgcgcg
     5041 gcactcgagg agtcgatccg gcgagcccgg atcgtccacc acgaccagcg ccgggccgac
     5101 gtcaccaccc gcgttgtcga aggtggcacc gtcaccgagc ggtgggtgcc ggtcgagcgg
     5161 gtcggcctct acgtccccgg tggcctcgcg gtctatccct ccagcgtcgt catgaacgtc
     5221 gttcccgcgc aggcggccgg agtcggctcg ctcgccgtca ccagcccgcc gcagcgcgag
     5281 aacgtcggcg tgttcaccgg ctaccccaac ccgacgatcc tcgccacgtg cgcgttgcta
     5341 ggggtcgacg aggtgtatgc cgccggcggc gcccaggcga tcgcgatgct cgcctacggc
     5401 ttcgacgaga ccgtcacgca cgccgatggc agcaccgaga ctctggtctg cgagccggtc
     5461 tccctcatca ccgggcccgg gtcgatctgg gtgacggccg ccaagcgtct cgtccagggc
     5521 ctggtcggca tcgacgccga ggccggaccg accgagattg ccgttcttgc tgacgacagc
     5581 gccgaccccg agcacgtcgc cgccgacctg ctcagccagg ccgagcacga ccccctcgcc
     5641 gcatccgtgc tcgtcaccgt cagcgaggcg ctcgccgatg ccgtcgatgc ggcgctggcc
     5701 cggcgggtcc ctgacgccaa gcacggagag cgcatccgtg aggcgctcac cggacgtcag
     5761 tcgcgggtcg tcctcgtcga cgacctcgat gccgggatcc tcgtctgcga tgtctatgcc
     5821 gccgagcacc tggagatcca gacccgcgac gccgccgagg tggccggccg gatccacaat
     5881 gccggcgcga tcttcgtcgg cccctggtca ccagtgagcc tgggcgacta tgccgccggg
     5941 tccaaccacg tcctgcccac ggcggggtcg gcggtccact ccagtgggct gtccgtgcag
     6001 tcgttcctgc ggggcatcca cgtcgtcgcc tacgacgagg cggcgttgcg tggggtcgcc
     6061 caccacgtcg tcacgctcgc ccaggccgag gacctgcccg gccacggcca ggccgtcacg
     6121 gcccggttcg gcgacgcggt gccgacgcca tgagcgcgcc ggcggtgccg accgacgtgg
     6181 cgcagcgggt cgccaggctc cttcgcgacg acctgcgtgg ccagacggca tacggggctc
     6241 cgcagttgga cgtccccgtg cgcctgaaca ccaacgagaa ctcctatgcc gtcccgcccg
     6301 acgtcgtcgc cgcgatcacc gctgccgtcg ccgggtgtgc gagcgacctc aaccgctacc
     6361 ccgaccggga gttcaccgcg ctgcgggagg acctcgctgc gtggctcacc gagcagaccg
     6421 gggtggcggt cgaggccgcg caggtgtggg cgggcaacgg ctccaacgaa gtgctccagc
     6481 acgtcgtaca ggctttcgcc gggccggggc gggtggcgct gggcttcacc ccggcctact
     6541 ccatgcaccc gatcatcagc cgaaccatgg gttcgacgtg ggtcgacgga caccgcggcg
     6601 ggccggacgg gcccttcgac ctgaccgagg gcgatgtcgt cgcccaggtg ctggccgcgg
     6661 ccccgcatgt cgtcttcctc tgctcgccca acaaccccac cggcaccgcg ctcgacctgg
     6721 agatcgtccg ggccgtgctc gacgcggcgc ccgacgcggt ggttgtcgtc gacgaggcgt
     6781 acgccgagtt cgccaggccc ggcacaccgt cggcactcag cctgctcgcg gcatacccgc
     6841 aactcgtggt gacccggacg atgagcaagg ctttcgcgct ggccgggggg cggttgggtt
     6901 atctcgtggc cgcgcccgag gtgatcgacg cgttgcgcct cgtgcggatg ccctaccacc
     6961 tctccacccc gacgcaggag atcgcccggg ccgccctggc gcaccgcttg agcctgctgg
     7021 cggaggtcga ggccatcaag aaccagcgcg atcgcatcgt ggaaaccctt gcagcacaag
     7081 gcttcccgcc cgtgcctagc gatgccaact tcgtcctgtt cggtgggttg gccgacgctg
     7141 gccgcacctg ggaggcgttg ctcgcccacg gtgtgctcgt ccgcgatgtc gggatcgccc
     7201 accaccttcg ggtgaccgct ggcacgcccg cggagacgac cgcgttcctc gacgcgatgg
     7261 ccgccctgcc tgccgagcac cggagccaga ccgcccccga accctctgcc gagccgtccg
     7321 ccagccaagg agcccctgca tgagcgacca gcccgcccgc cgcgccaccg tgcgtcgggc
     7381 cacgagcgag tcgagtgtcg agctcaccgt cgatctcgac ggcaccggcc gcagcaccgt
     7441 gtcgaccggg gtcgggttct acgaccacat gctcgcctcg ctggccaagc acgccctgct
     7501 cgacctcgat gtcgcggcgg tcggggatgt cgacatcgac gcccaccaca ccgtcgagga
     7561 cgtcgccatc tgcctcggtg atgcgctccg tgaggcgctg ggggacaagc gcgggatcag
     7621 ccgcttcggt gacgcgacgg tgccgctgga cgaggcgttg gtccaggccg tcgtcgatgt
     7681 cgccgggcgc ccctactgcg tccacacagg ggagccggcc gggcaggagt tcgtcgtcat
     7741 cggcgggtcc tacatcgggt cgttgacccg ccacgtcttc gagactctgg cccaccacgc
     7801 cggcatcgcc ctgcatgtgc gggtgctcgc cggacgcgac cctcaccaca tcgtcgaggc
     7861 ccagttcaag gccgtggcta gggccctgcg ggctgctgtc gcccccgatc cgcgggtgca
     7921 cggcataccc agtgccaagg ggacgctgtc gcggtgagcc ccgacgcggg cctcggtgac
     7981 ggcgcggcct cgggagcggg gtcgagcggc ggcggtctgc tgctcgttcg tgccggggtg
     8041 tcgacggtgc gggagtgggc gctggacggg ctcggcccgg tcatcgtcgt gcctgacccg
     8101 agcgggtgga cgatcctggt cccggacggg gaccgaacct tcgcggcgcc gcccttcgac
     8161 gacggcgtgg gtgccctgct cggtcgaccc gtgcggcggc gactgctacc gacgatcggg
     8221 gtcgcgctgg tcggcaaccg attggcgatc agcgtcgtcc cggcggtgtt gcgaccgggg
     8281 cggtcgtggc tggtctggga gccgggggca gggctggtcc gcccggccgg cctcaccccg
     8341 gcaagcgtcg cgcaactggc ggctgcggcc gaccgccgcg agcaccgcga cctcgtcgca
     8401 gcgatgatgg ccgatggccg tggctcggcc gctcgggtcc tgcgggatgt cttcgaggcg
     8461 ctcagccttc ccggggccga gttcgccacc ggcgagcgag tgccagtgga tgaaccgggt
     8521 gccgtgcgca tcgagccgtc gcgagaagat gtcgccaggt tcgatcgcac ggtgcacgag
     8581 gttcggcgct ggcgcgagga ggtggaaggg agcccatgag tcccacagtg gtcgttttcg
     8641 actatggcag tggcaacgtc cgctcggcgg tccggtccct cgagcgggtc ggtgccgagg
     8701 tcaccctcac ggcacacgcc gacgaggcgc tggccgccga tggtctggtc gtcccggggg
     8761 tgggcaactt ccatgcctgc atggccgggc tgcgtgctgc tgacggtgct cggatcatcg
     8821 aggtgcggct ggccggcaat cggccggtgc tgggcgtctg cgtcgggatg caggtgctct
     8881 tcgacggctc caccgagccc gccgcccacg aggagcccgg cttgggggag tggcccggca
     8941 tggtcgagcg gctcgtcgct ccggtcgtcc cccacatggg atggtcgccc gtgcaggtcc
     9001 ccgacgggtc gcggctgttc gccggcgtcg agtccgagcg cttctacttc gtccactcgt
     9061 atgccgtgca gtcctggacg cttgaaccgc acggaccctt cgcccctcga gcgccgatgg
     9121 tcacctggtc cgagcacgga gcgccgttcg tcgctgctgt cgagaacggt ccgctggcgg
     9181 ccacgcagtt ccaccccgag aagtccggcg atgccggcgc acacctgctg gagaactggg
     9241 tgaggtccct atgaacgcca aacgcgcctc caacacccgc cggacgacca ccaccagacg
     9301 acgcaggtcg acgacgacgt cctcggggta tcggcgtcgt cgccgccccg gcctgtcgtc
     9361 gacggtcggc tcggcgatcg ggatgctcgc cgtgacgacc ctgctcaacc tgtcgtggcc
     9421 ggctcggatc gggctgctcg tgctggtcct cgtggtcggc ctggcctaca tgctctggcg
     9481 gcaccgggcc gagatcgcgg cgggggcgga cggcataccc gaggacgcag cggcaccacc
     9541 caccccgcag gaccgaccca cccccagccc ggaagagaac ccgtcgtgac cgagtcgccc
     9601 cgactgcaac tgctgcccgc cgtcgacatc tccgacggag gtgccgtgca actgcagcag
     9661 ggcgtcgccg gctcggggtg gaagttcggc gacccctggc tggccgcgaa gcgttggcag
     9721 gacgacggcg ccgagtggat ccacctggtc gatctcgacg cggccttcgg ccgagggaac
     9781 aaccgcgaat tgatcgctga catcgtcggc cggctggacc ttcaggtgga actctccggc
     9841 gggatccgcg acgccgattc gctggccgtt gccctcgcga ccggctgccg tcgggtcaac
     9901 gtcggcaccg ccgccttgga gaacccggag tggactgccc gcgcgatcgc cgagcacggg
     9961 gaccgggtcg ccgtcggcct cgacgtccgt ggcacccggc tggccgcgcg cggttggacc
    10021 tcggagggcg gcgacgtgtg ggagaccctg gaccggctcg acgccgaggg ctgctcgcgc
    10081 tacgtcgtca ccgacgtcac caaggacggc atgctggccg gccccaacct cgccctgctg
    10141 cgtgaggtcg cggcccggac cgaccggcc
//
LOCUS       DAORPW010000081        10112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1525_length_10112_cov_836.540320, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000081 DAORPW010000000
VERSION     DAORPW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1525_length_10112_cov_836.540320"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1263)
                     /locus_tag="PLB86_07880"
     CDS             complement(64..1263)
                     /locus_tag="PLB86_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC65095.1"
                     /translation="MPELPTPGEVTLVTVGLLAALLLGAVVMRLVGRRRFVDPVDRAT
                     YETLHTASLATRSLRDGLTPEGATRATRHLRSMLGSTAVAIVDTATTLAWDGAGSHHR
                     GDVRAHAEPVLSSGRTLVLTSDQITCSDPDCSIRSGIIAPIVADDRVVAALAAYGRTT
                     STGLVRATEELAGWISTQIELAELGRQRTRVMEAELRALRAQISPHFIYNCLGAIASF
                     VRTDPDRARELLLEFADFTRYALRRGGAYTTLRDELQNIERYLALENARFGDRLVVSL
                     TIAPEVLAVSVPFLAIQPLVENAVRHGIEHKAGIGHVTITASDVGANAEITIEDDGAG
                     SDPDTIRAVLAGESSRDSVGLGNVDARLRQAFGDEYGLVVETAPGAGTKVTFRVPKFA
                     PGVHSGP"
     gene            1307..2149
                     /locus_tag="PLB86_07885"
     CDS             1307..2149
                     /locus_tag="PLB86_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRC65096.1"
                     /translation="MSDARAAVLTLLDLEGVREAVDAARDACTQLRWHEALRRRIPEA
                     SAESRVRGSRASAFLDGAELPVDLVRDLVRGARPWPEHLDPVEEVLRGAIQATAETEH
                     IRGLLVRSPSQALARLHVAAASGLLPVEQVGRPRQGEETSREFVDLGMPPAPAELPAR
                     LAGVAELIGLAGEVPVVLAAALVHAELAVVRPFVRGNGLVARAAERALVAAAGLDPTG
                     VAVPEAGWLAGGGTAYLGALTAYASGTPQGVALWLRQAAQAMTQAAAEGARVADAVRV
                     GRLT"
     gene            2597..3412
                     /locus_tag="PLB86_07890"
     CDS             2597..3412
                     /locus_tag="PLB86_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65097.1"
                     /translation="MDGSAGRSARGVVLAVVGASGGCGASTLAAACALHAAESGWSVG
                     VVDGAAGGAGIDVVLGIEHVPGVRWPDLAGCRGRVDGLGLLSRMPRAGAVAVLAHCRD
                     SPAQIGADAVRGVFAGLVEVLDLVVVDVPRSWALQDWALPDGSLREAAVREAALLDFA
                     PREAALPSLRAVVVAGAGIVELAALAATAPRVAARVPETYLVLRGRRLSRSLLDDVGA
                     ALDLPVLGVLDDDPSVGRDLARGIPPGRSRGPLADLARAVVEALGGDRREAAS"
     gene            3409..>4307
                     /locus_tag="PLB86_07895"
     CDS             3409..>4307
                     /locus_tag="PLB86_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HRC65098.1"
                     /translation="MTLDPMVWEQIRAGRAPDAEVVERIARSELAVLGSAGAADAGAR
                     LSASVLGAGPLERLTEDPAVTDIAVNGDGRVWVDRGAGMEQVAVSLGSVAVRRAMAVR
                     LAALAGRRLDDTAAYVDAVLPTGVRLHAILPPLVSGGPHVTLRVPARVPLSFEDLVAA
                     GSVPVGWGPVLRGLVERRVSFLVSGGTGAGKTTLLGALLGLADQRERILVVEDVRELA
                     VEHPHVVHLEGRSPNVEGVGEVTMVTLLRQALRMRPDRIVVGEVRGAEVRELLSALNT
                     GHEGGAGTIHANAASDVVARIEAL"
     assembly_gap    4308..4407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4408..4976
                     /locus_tag="PLB86_07900"
     CDS             <4408..4976
                     /locus_tag="PLB86_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65099.1"
                     /translation="AIVRSWWSRRPSARERARSALVAQTCEALDLCVAGLRAGLDLPA
                     VLDFADAEIGLSAEVRDALRAPPGRLASGQVFRVISQAQELSSSAGVPLAEAWESAAA
                     LLREHEGLRRKIAVALAGPRATMRVLTLLPLAGPVVGIVFGIDPWRLYSGSPITVACL
                     GLGLGLLFVGRWWCARLVSRLGLAQALG"
     gene            4973..5521
                     /locus_tag="PLB86_07905"
                     /pseudo
     CDS             4973..5521
                     /locus_tag="PLB86_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
     gene            complement(5482..7266)
                     /locus_tag="PLB86_07910"
     CDS             complement(5482..7266)
                     /locus_tag="PLB86_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC65100.1"
                     /translation="MRTVVVIGGVAGGMSAAARLRRLDPSARIVVVEQGSEVSFANCG
                     LPYFLSGEIANRSDLLLRTPGSLAATLSLEVRVDTLATAIDPAAREVTVRGPEGREER
                     IGYDALVLAPGAYAVRPPIPGIDHPRVRTLRTVPDADTIRGWVQQGAARAVVLGAGFI
                     GLEAAEALRHAGLDVSIVEAAPHVLPVIDAELAAYVTDELRTHGVNVHEGVAARAILD
                     VEGTPVVELADGTVLPADLVILSVGVRPRSELAAAAGLALTEQGAIIVDDDQRTSDPH
                     IWAVGDATASTHAITGAVAPVALATPANRGGRVAADSIAGGGRRSPRPLGTAVVRVFD
                     LTIAMTGASRRSLGDRPFHTVRVHAGHHAGYYPGAETMHLLLHFDGEGRILGAQGVGR
                     EGVDKRIDVIATAARGGLRVDDLVDLDLSYAPPFGAAKDPVMMLGMIADNVLSGRLTQ
                     HDPVAPLDPETTLVLDVRNPGEWDRGHIEGAMLVPQPDVAASIEEIRRAAAGRPIVVH
                     CASGFRSYLANRTLAGAGIDSANVSGGFTTLAIAQPQLVAHPQTSPASATTSGTTVSS
                     TNAGRNANPSGSTIVTPSLAAASSISCS"
     gene            7465..7668
                     /locus_tag="PLB86_07915"
     CDS             7465..7668
                     /locus_tag="PLB86_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HRC65101.1"
                     /translation="MGKALRWKLIDGWREARRRMDAGMTTAEYAVGTLAAVAFAAALI
                     AVVKSDAVRSALARIIQSALSTG"
     gene            7712..8038
                     /locus_tag="PLB86_07920"
     CDS             7712..8038
                     /locus_tag="PLB86_07920"
                     /inference="COORDINATES: protein motif:HMM:NF041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HRC65102.1"
                     /translation="MATAEFAVALPAVVLVLVVALSAIGAGIDQIRCVDAARLAARAL
                     ARGDSQGAAAALARGAAPSGASVTLSAGGDLATAMVSVSRRAPVVGFSWVVAASATAP
                     REAVPP"
     gene            8035..8379
                     /locus_tag="PLB86_07925"
     CDS             8035..8379
                     /locus_tag="PLB86_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65103.1"
                     /translation="MSRERGQSTVAVLGVLGALVASTVGALDLARAVNAAHVARAAAD
                     LAALAGAGAGVRGAGAGEACAQASRLAALNHAQLVACAVPGEGSVEVSVTATSGGPWP
                     RTARASARAGPG"
     gene            complement(8393..8692)
                     /locus_tag="PLB86_07930"
     CDS             complement(8393..8692)
                     /locus_tag="PLB86_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65104.1"
                     /translation="MVAFGLILLLIGVGAGGWLAWLALQSETGVTLGATGLQLTVLPI
                     TLFVAGAASMLLLWLGVRFMALGTKRRVARRKELKELRTAPPASGGAGPRSTDGL"
     gene            complement(8761..>10112)
                     /locus_tag="PLB86_07935"
     CDS             complement(8761..>10112)
                     /locus_tag="PLB86_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HRC65105.1"
                     /translation="VGSGAVIAAYRGGYLPEERRILEGQLRSGHLRGLAATTALELGI
                     DVSGLDAVLLAGWPGTVSSFWQQVGRAGRAGRESLAVLVAADDPLDGFLVRNPEAIFD
                     RPVEVAVVDPDNPHVLAPHLVCAAAELPVTDADLAPDLFGPGAGPLLDRLVADGVVRR
                     RPTGVYWARDEPPAASVSLRGIGQTVRVVERSTGRVVGLVDESRADAAVHPGAVHVHQ
                     GRSFVVTALDLDEGSALVVAGDPGWSTQARSVSEFNLGKVREQVSSGPLTAAFGEVTV
                     HHQVMSFLRRLPDGEVIGEHPLDLPVRTLSTTGIWWTLPEETLLDVGVDASGIPGALH
                     AAEHAAIGMLPLVASADRWDIGGVSTARHPDTGLPTVLVYDGYPGGAGFAQRGYAALH
                     DWLAATRDVVAGCACRDGCPSCVQSPKCGNGNHPLDKAGAVRVLAHVVSLLERCGTE"
BASE COUNT         1243 a         3369 c         3885 g         1515 t
ORIGIN      
        1 tacaccctca tacaagtgcc accaccctca tacaagcggc gcgtctgccg gcgcccgttc
       61 acctcagggg ccggagtgga ctccaggcgc gaacttggga acgcggaagg tgaccttggt
      121 gccggcgccg ggggcggtct cgacgaccag gccgtattca tccccgaagg cctgccgcaa
      181 ccgggcgtcg acattgccca ggccgacgct gtcccgcgag ctctcgccgg ccagcacagc
      241 ccggatcgtg tcggggtcgc tgccggcgcc gtcgtcctcg atggtgatct cggcgttcgc
      301 gccgacgtcg gacgcggtga tggtgacgtg gccgatcccg gccttgtgct cgatgccgtg
      361 ccgcacggcg ttctcgacga gcggctggat cgccaggaac ggcaccgaca ccgcgagcac
      421 ctcgggcgcg atggtgagcg acacgacgag gcggtcaccg aaccgggcgt tctccagcgc
      481 gaggtagcgc tcgatgttct gcaactcatc gcgcagggtc gtgtaggcac cgccgcggcg
      541 caacgcgtag cgggtgaagt cggcgaactc cagcaacagc tcgcgggccc gatcagggtc
      601 ggtccggacg aaggacgcga tcgcgcccag gcagttgtag atgaagtgcg gcgaaatctg
      661 cgcgcgcaag gcccgcagct cggcctccat cacccgggtg cgttggcgac ccagctcggc
      721 gagctcgatc tgggtggaga tccacccagc gagctcctcg gtggcccgga ccagcccggt
      781 cgaggtggtt cgtccgtatg ccgcgagcgc cgccaccacg cggtcgtcgg cgacgatcgg
      841 cgcgatgatc cccgagcgga tcgagcagtc ggggtccgag caggtgatct ggtcggaggt
      901 gagcacgagg gtgcgccccg aactgagcac cggctcggcg tgggcgcgga catcgccgcg
      961 gtggtgggac ccggcgccgt cccaggcgag ggtggtggcc gtgtcgacga tcgccacggc
     1021 ggtggagccg agcatcgagc gcaggtgccg ggtggctcgg gtggctccct cgggggtgag
     1081 tccgtcgcga agtgatctcg tggccagcga cgccgtgtgg agggtttcgt aagtcgcccg
     1141 gtcgacaggg tcgacgaacc ggcgcctccc gaccagccgc atgacgacgg cgccgagcaa
     1201 cagggcagcg agcagcccga cggtgacgag ggtgacctcc ccgggagtgg gcagctcggg
     1261 catggcctca ctctaggagg cgcgcggacg gcatacgctg cggttcgtgt ccgatgctcg
     1321 cgctgctgtc ctgaccctgc tggatcttga gggggtccgc gaggcggtcg acgccgcgcg
     1381 cgacgcctgc acccaactgc gctggcacga agcgctgcgc cgacggatcc ccgaagcgtc
     1441 ggccgagtcg cgggttcgtg gctcccgggc gagcgcgttc ctcgacgggg ccgagctgcc
     1501 cgtcgacctg gtccgtgacc tcgtgcgggg cgcgcgtccg tggcccgagc acctcgaccc
     1561 ggtcgaggag gtgctgcgcg gggcgatcca ggcgaccgcc gagaccgagc acatcagggg
     1621 tctgctcgtg cgctcaccat cgcaggcgtt ggctcggctg catgtcgccg cggcctcggg
     1681 gctgctgccg gtcgagcagg tggggcgccc gcgccagggc gaggagactt cccgcgagtt
     1741 cgtcgatctc ggtatgccgc ccgcccccgc cgaactcccg gctcgcctgg ccggggtggc
     1801 cgagttgatc gggctggccg gcgaggtccc cgtcgtcctg gcggcagcgc tcgtgcacgc
     1861 cgagttggcc gtggtgcgac cgttcgtccg gggcaacggt ctggtggcgc gggctgccga
     1921 acgggccctc gtggcggccg ccgggctcga ccccaccgga gtggctgtgc ctgaggcggg
     1981 gtggctcgcg ggtggtggga cggcctacct cggggcgctc acggcatacg cctcggggac
     2041 tccgcaaggc gtggccctgt ggttgcgcca ggccgcgcag gcgatgaccc aagcggcggc
     2101 cgagggcgcg cgggtcgccg acgcggttcg ggtcggtcgg ctgacctgag cgtgcggggc
     2161 cgcttgggga ctgtgtcgag gccgacacgc caagcgtgtg ttgcgaatgg ggtaccgttt
     2221 gcctttcggg ctcggaagcc ggagaatagc catgctcccc ctcggggttg agcaggtgcg
     2281 cactggcccc tcccccccgg tgccagcgcg ccagacggcc cccgttgtcc ccccagcggg
     2341 ggccgtcgca cgtccccctt ccggccccgg ccagtggtgt ggtgccctct ttctgccgtc
     2401 agtgggctga ggtggagtcc gcggcgtcgt gctgtcggtg ggccaaggcg tgggcgcggc
     2461 ggtgggtgtc aggatcacca tcggcgggtc gcgcgcttgc tgagttgtcc acatcccctt
     2521 ggtcatccgt cccaccatcc acagggccgc cggtggtggg tgcgggccgg gacgggcgca
     2581 ggcgaggctg gccggcatgg acggatctgc tggcaggagc gcgaggggtg tcgtcctcgc
     2641 ggtcgtgggt gcctcgggag gctgcggggc gtcgacgctc gccgcagcgt gtgccctgca
     2701 cgccgcagaa tcgggctggt cggtgggcgt ggtcgacggg gctgctggcg gtgccgggat
     2761 cgacgtggtt ctcggcatcg agcacgttcc gggcgtgcgg tggcccgacc tcgcggggtg
     2821 ccgcgggcgg gtcgacgggc tggggctgct ctcccgtatg ccgcgcgccg gcgccgtcgc
     2881 ggtgctcgcc cactgccgcg actcgccggc gcagatcggg gcggacgcgg tccgtggggt
     2941 gttcgcgggt ctggtggagg ttctcgacct ggtggtcgtc gatgtgccgc gctcgtgggc
     3001 tctgcaggac tgggctctgc cggacgggtc cttgcgggag gcggctgtgc gggaggcggc
     3061 tctgctggat ttcgctccgc gggaggcagc gctgccgtcg ctgcgtgcgg tcgtcgttgc
     3121 gggcgcgggg atcgttgagc tggcagcgct cgcggccacg gctccaagag tggcggctcg
     3181 cgtgcccgag acctatctcg tgctgcgcgg tcggcggttg tcccgatcgc tcctcgacga
     3241 cgttggggca gccctcgacc tgccggtcct tggcgtgctc gacgacgacc cgagcgtcgg
     3301 gcgggacctg gcgcgcggca tacccccggg tcgtagccgt gggccactgg cggatctcgc
     3361 gcgggcggtc gtggaagcgc tgggcgggga tcggcgcgag gcggcctcat gacccttgac
     3421 ccgatggtgt gggagcagat ccgcgccggc cgagcgcccg acgcagaggt ggtcgagcgg
     3481 atcgcgagat ccgagttggc ggtgcttggg tcggcgggag cggccgacgc tggagcacgg
     3541 ctgtccgctt ccgtgttggg tgcggggccg ttggagcgtt tgacggaaga ccctgcggtc
     3601 accgacatcg cggtcaacgg cgacggtcgg gtctgggtgg accggggcgc cgggatggag
     3661 caggttgcgg tgtcgctagg gtcggtggct gtccgccggg cgatggcggt gcggctggcg
     3721 gcgctggccg ggcgccgcct ggatgacacc gcggcatacg tggatgcggt cctgccgacg
     3781 ggggtgcggc tgcatgcgat cctgccgccg ctggtgtccg gtggtcctca cgtgacgttg
     3841 cgggtgccgg cgcgcgtccc gttgtccttc gaggacctcg tcgctgccgg gagtgtgccg
     3901 gttgggtggg gcccggtgct tcgcgggttg gtggagcgac gggtgtcgtt ccttgtcagc
     3961 ggcggcaccg gggccgggaa gacgacgctg ctcggcgcgc tgttggggct ggccgatcag
     4021 cgggaacgga tcctcgtggt cgaggacgtg cgcgagttgg ctgtcgagca tccgcatgtc
     4081 gtccatctgg agggtcgttc acccaatgtc gaaggggtcg gcgaggtgac gatggtgacg
     4141 ttgttgcgtc aggccctgcg gatgcgtccg gatcggatcg tcgtcggaga ggtgcgtggt
     4201 gccgaggtcc gtgagttgct gtcggcgctc aacaccgggc atgaaggcgg cgcgggcacg
     4261 atccacgcga atgccgcctc cgatgtcgtc gcccgcatcg aagcgctnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnncag cgatcgtccg cagttggtgg tcgcgccgtc
     4441 cgagcgcccg ggagcgggcc cgatcagccc tggtggcgca gacctgcgaa gccctcgacc
     4501 tgtgcgtggc ggggctgcgc gccggactgg acctgccggc cgttctcgac ttcgcggacg
     4561 ccgagatcgg tctgagcgcc gaggttcgtg atgcgctacg tgcgcccccg ggtcgccttg
     4621 cgagtggtca ggtcttccga gtgatcagtc aggcccagga gttgtccagc tcggcgggtg
     4681 tcccgctggc tgaggcgtgg gagagcgccg cagccctgct gcgggagcac gaggggctgc
     4741 ggcgcaagat cgccgtggcc ctcgccggcc cgcgcgcgac gatgcgggtg ctcacgctgc
     4801 tgcccctcgc cggtccggtg gtgggcatcg tgttcggcat cgacccgtgg cggctctatt
     4861 cagggtcacc gatcaccgtc gcctgtctcg gccttgggct gggactgctg ttcgtcgggc
     4921 gatggtggtg cgcccgcctc gtcagccgcc ttggcttggc tcaggcgctg ggatgacggc
     4981 tggcgtgccg acatcagtgg ccgaagcctt cgccggggtg gaaggcctgg tggcgctcct
     5041 gctggccggc gcggtcctcg cctggccgtg gcggtccaca gccctccccg cgggcctgga
     5101 ctcaagcgcc cgcctggccc ggcccgtgcc agatgccgca aggccgaggt cggtgcgggc
     5161 agtcgggcgg cggcacggca tacctggtgc gcctgctgcc cccgcgatag ccgacgccct
     5221 ggagttggtg gcgctcgtcc tcggggccgg cggcggcctc gtcgatgcgg tcgaggcggt
     5281 cgcgcggacg tcgaagggcg tggtgtgcga gcacctcacc atcgtcgccg cggccgtgcg
     5341 gtggggtatg ccgtggtccg tcgcctgggg ggccgtcgac gacgcgtggg caccggcacg
     5401 ccgagcgatg gcgctcgccg acgcggcggg ggtcgccccg tcggcggcgt tacgtgccgc
     5461 cgccgccgac ctgcgcgcca ctcagctgca agagatggag ctcgcggccg ccaggctggg
     5521 cgtgacaatc gtgctgccgc tggggttggc gttcctgccg gcgttcgtgc tgctgaccgt
     5581 ggtgccgctg gtcgtcgcgc tggccgggga ggtctggggg tgagcgacga gttgaggctg
     5641 ggcgatcgcg agcgtcgtga agccgccgga cacgttggcg ctgtcgattc ccgctccggc
     5701 cagcgtgcgg ttggccaggt aggagcggaa ccccgacgcg cagtgaacga cgatgggccg
     5761 accggccgcg gcccgccgga tctcctcgat cgacgccgcg acgtcgggct gcggcacgag
     5821 catcgcgccc tcgatgtgtc cgcggtccca ttcgccgggg ttccgcacgt cgagcacgag
     5881 cgtggtctcg ggatccagcg gagcgaccgg gtcgtgctgg gtgaggcggc cggacaacac
     5941 gttgtcggcg atcatcccca gcatcatcac cgggtccttc gccgcaccga agggcggggc
     6001 ataggacagg tcgagatcga ccagatcgtc gacccgcaac ccgccccggg cggcggtggc
     6061 gatgacgtcg atgcgcttgt cgacgccctc gcggccgact ccctgggctc cgaggatgcg
     6121 gccctcgccg tcgaagtgca gcagcaggtg catcgtctcg gcgccggggt agtagccggc
     6181 gtggtgaccc gcgtgcaccc ggacggtgtg gaagggccgg tctcccaagg agcgccgaga
     6241 agcgcctgtc atggcgatgg tgaggtcgaa gacccgcacg acggccgtgc cgaggggccg
     6301 gggtgagcgc cgcccacccc ccgcaatcga gtcggcggcc actcggccac cccggttggc
     6361 gggggtggcc agggcgacgg gagccacggc tccggtgatc gcgtgcgtgc tggccgtggc
     6421 gtcgccgacg gcccagatgt gtgggtcgga ggtgcgctgg tcgtcatcga cgatgatcgc
     6481 cccctgctcg gtgagggcca ggccggcggc cgcagcgagc tctgagcgtg gcctgacccc
     6541 gaccgacagg atgaccaggt cggcgggcag aaccgtgccg tcggcgagct cgacgaccgg
     6601 ggtgccctcg acgtcgagga tggcgcgagc ggcgactccc tcgtgcacgt tgaccccgtg
     6661 ggtgcgcagt tcatcggtga cgtatgccgc gagctcggca tcgatgactg ggagcacgtg
     6721 gggggccgct tcgacgatgc tcacgtccag gccggcgtgg cgcagggcct cggcggcctc
     6781 caggccgatg aacccggcgc ccaggacgac ggcgcgtgcg gcgccctgct gaacccagcc
     6841 gcggatcgtg tccgcgtcgg ggaccgtccg aagggtccgc acccgcgggt ggtcgatgcc
     6901 ggggatcggt gggcggacgg catacgcccc gggggccagg acgagcgcgt cgtagccgat
     6961 gcgctcctcg cggccttcgg gaccgcgcac ggtgacctca cgggccgccg ggtcgatcgc
     7021 ggtggcgagg gtgtcgacgc ggacctcgag gctgagggtg gcggcgagtg accccggcgt
     7081 gcgcaggagc aggtcgctgc ggttggcgat ctcgccgctg aggaagtagg ggagcccgca
     7141 gttggcgaac gacacctccg agccctgctc gacgacgacg atgcgcgcgc tggggtccag
     7201 tcggcgcagc cgcgcggcgg cactcatccc cccggcgact ccaccgatga ccacgacggt
     7261 tctcacgact gccacgctcc ttgactcttc agttcgggtt gaagcatacc cctaggggta
     7321 tgcagcgcgc gcgcgggtcc gggttgtcca cacccgcctg gtcagttgtc ccatcatcca
     7381 caggccgggc gggaggcgct gacaacaggt cgggtcggcg gtgaggatcg agctatcggt
     7441 ctgcgactga agggacgtgt gatgatggga aaagccttga ggtggaagct gattgacggc
     7501 tggcgtgagg ctcgccgccg catggacgcc ggcatgacga cggcggagta tgccgtgggc
     7561 acgttggccg cggtggcgtt cgccgcggcg ctgatcgcgg tcgtgaaatc cgatgcggtg
     7621 cgttcggcct tggctcgaat catccagtcc gcgctgagca cggggtgagg gcgccccggt
     7681 gaggcggccg cgtggtcgga gcgagcgcgg gatggcgacg gccgagttcg cggtggcgct
     7741 gcctgcggtg gtgctcgtgt tggtggtggc gctgagcgct atcggagccg ggatcgacca
     7801 gatccggtgt gtcgatgccg cccggcttgc agcgcgggcg ttggccaggg gcgactccca
     7861 gggcgccgcg gccgcgttgg cgcgtggcgc ggctccctcg ggtgcatcag tgaccctgag
     7921 cgccggtggc gacctggcga cagcgatggt ctcggtgtcg cgccgggcgc ccgtggtggg
     7981 cttctcctgg gtcgtggcgg cgtcggcgac tgctccccgt gaggcggttc cgccgtgagc
     8041 cgtgagcgtg gtcagagcac cgtggcagtg ctcggagtcc tgggcgcgct ggtggcgtca
     8101 acggtcggag cgctcgacct ggccagagcg gtcaacgccg cccacgtcgc gcgggctgcc
     8161 gctgatctcg ccgcgctggc gggtgcgggc gcaggggttc gcggtgctgg cgcgggagag
     8221 gcctgcgctc aggcctcccg gttggccgcg ctcaaccacg cgcagctcgt ggcgtgtgca
     8281 gtgccgggcg agggttcggt cgaagtctcc gtcacggcga cttcgggcgg gccgtggccg
     8341 aggacggccc gagcctccgc gcgggctggc cctggctgag cccgtcggga ccttagagac
     8401 cgtcagtgct tcgcggaccc gcgccaccgg aggccggggg cgcggtgcgc aactccttga
     8461 gttccttgcg ccgggcgacc cgacgcttgg tcccgagggc catgaatcgc accccgagcc
     8521 acaacagcaa catcgacgcg gcaccggcga cgaacagcgt gatcggcagc acggtcaact
     8581 gcagcccggt cgctcccagc gtcaccccgg tctcgctctg caaggccaac cacgcgagcc
     8641 agccacccgc gcccaccccg atgaggagca ggatcaaccc gaatgcgacc acgttgtcct
     8701 cctgatgtcc cacccacgcc gtcgaccgcg gccggcgatt cgacggcttc ggtggcgaca
     8761 ctactcagtg ccgcaacgtt ccagcaggct caccacgtgg gcaaggaccc gcacggcgcc
     8821 cgctttgtcc agcggatggt tgccgttgcc acacttgggc gactggacgc acgacgggca
     8881 gccgtcccgg caggcgcacc ccgccacgac gtcacgggtc gccgcgagcc agtcgtgcag
     8941 ggcggcatac ccgcgctgcg cgaacccggc cccgcccggg tagccgtcgt agacgagcac
     9001 cgtcggcaac cccgtgtccg ggtgccgcgc ggtcgacacc ccaccaatgt cccagcggtc
     9061 ggcgctcgcc acgaggggca gcatcccgat cgccgcgtgc tcggcggcgt gcagagcccc
     9121 cggtatgccg ctcgcgtcca cgcccacgtc gagcagcgtc tcctcgggga gggtccacca
     9181 gatgccggtc gtcgacaggg tgcggaccgg gaggtcgagt gggtgctccc cgatgacctc
     9241 gccgtcgggc agtcggcgca ggaaggacat cacttggtgg tgcacggtca cctcgccgaa
     9301 cgcggcggtc aacgggccgc tcgacacctg ctcacgcacc ttccccaggt tgaactcgct
     9361 caccgacctc gcctgcgtgc tccagcccgg gtcgccggcg acgacgaggg ccgagccttc
     9421 atcgaggtcg agcgcggtga cgacgaaact gcgcccctgg tggacgtgga cggcgcctgg
     9481 gtggaccgcc gcgtcggcgc gcgactcgtc gacgaggccc acgacccggc cggtcgagcg
     9541 ctccacgacc cgcacggtct gcccgatccc gcgcaacgac accgacgcag ccggcggctc
     9601 gtcgcgggcc cagtagacgc cggtgggtcg gcgccgcacg acaccgtctg ccaccaaccg
     9661 gtcaagcagc ggcccggcac ccggcccgaa caggtccggc gcgaggtcgg cgtcggtcac
     9721 cggcaattcg gccgccgcgc agacgaggtg gggggccaag acatgtgggt tgtccgggtc
     9781 gacgaccgcg acctcgacag gccggtcgaa gatggcctcc gggttgcgca cgaggaagcc
     9841 gtccagcggg tcgtcggcgg cgacgagcac cgccagcgac tcgcggccgg cccgcccggc
     9901 tcggcccacc tgttgccaga acgacgacac cgtccccggc caacccgcca ggagcaccgc
     9961 gtccaggccg ctgacatcga tgccgagttc gagcgcggtc gtcgctgcga ggccgcgcag
    10021 gtgaccggaa cgcaactgcc cctccaagat ccgccgttct tcgggcagat acccgcctcg
    10081 gtatgccgcg atcaccgctc ccgacccgac gg
//
LOCUS       DAORPW010000082         9802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1604_length_9802_cov_819.246537, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000082 DAORPW010000000
VERSION     DAORPW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9802
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1604_length_9802_cov_819.246537"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2156)
                     /gene="pepN"
                     /locus_tag="PLB86_07940"
     CDS             complement(<1..2156)
                     /gene="pepN"
                     /locus_tag="PLB86_07940"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HRC65106.1"
                     /translation="MPGTNLTRDEAASRAALLHVNSYDVVLDLTTGPTTFLTSTTVRF
                     AARAAGVGTWIDFVGDSVTSVTLNGVALDPAEVFADSRIALPALAPDNVLVVDAVGRY
                     TNSGEGLHRFVDPVDNEVYLYSQFEVPDSRRVYAVFEQPDLKATFAFTATVPDHWVVV
                     SNQPAPEPVPAGEGRATWAFEPTPRISSYITALCAGPYTVRWDEVQGRDAVIPMGIFA
                     RRSITEYVDYGNLFDCTKAGILFFEKEFDGAFPFAKYDQLFVPEYNAGAMENAGCVTI
                     TDAYVFRGQPSEPLVERRALTILHELAHMWFGDLVTMKWWDDLWLNESFAEWASSVCQ
                     AEATEWPDAWTTFHSHEKTWAYQQDQQSDTHPVYADMRDLVDVTVNFDGITYAKGGSV
                     LKQLVAYVGRDAFREGLRSYFRKHAWGNTTMGDLFAEFEAGSGRDLSDWAQRWLKTSG
                     PSTLALDITTDDNGVITAAAMTQTVAGSDPTLRPHRVGIGRYDLLGGRLDRVGYVEVD
                     VDGPRTELPELVGLPRPALLLPNDEDLTYAKIALDPLSLQTAMAHIDAFDDSLARSLV
                     LGALWEMLRDGALAPSDFVDVLLRVIPVEEHTVVLRTILRTTKQIPSMLLATLRWFLP
                     EAARPAMVDKSVDVIRTALDAAVPGSDKQQQLVVAYAALARHTDDLTRLQGILDGSVV
                     VPGLSVDQDLRWALLTQIAASGGASEDDIAAELDRDP"
     gene            2308..2946
                     /locus_tag="PLB86_07945"
     CDS             2308..2946
                     /locus_tag="PLB86_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRC65107.1"
                     /translation="MTASPEARPTRVRRQPVEMWFDPMCPWAWMTSRWLMQVETVRPV
                     RVTWSVMSLAVLNEGRDLPEQYRALMEHAWGPVRVIIAAQAAHGDKVIKKLYDALGTR
                     IHLQARTDYDQVIAEALAECKLPAELAKAAHQKKYDAALKRSHKRAISLVGTDVGTPV
                     ISVGGVAFFGPVVSPAPKGEAAGRLWDGCVLVAGTPGFFELKRTRSIGPIFD"
     gene            2962..3426
                     /locus_tag="PLB86_07950"
     CDS             2962..3426
                     /locus_tag="PLB86_07950"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HRC65108.1"
                     /translation="MRVHLGGDHAAYALQRALVDHLTAAGHEVIDHGPLAYDAMDDYP
                     VFVLRAAQAVAADPGSAGIVLGGSGNGEVMAANKVPGIRAAYAASIELAELAREHNDA
                     QIISMGGRFTDIETGCAIADAFLATDFSGDERHARRLAMVSRFEADGSLPEG"
     gene            3435..4562
                     /locus_tag="PLB86_07955"
     CDS             3435..4562
                     /locus_tag="PLB86_07955"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRC65109.1"
                     /translation="MTAQTARREVDPGSTRVSVLRAEPWPARLRVVLQARQPVRAFGL
                     VVAGWVLLACMVAWPLNVAWSMYAPLVVLAGLFLTPGWFAVVSASYAVCLGISAFMVR
                     GWSVLQGFTLLALALTMTLMVMRTRSRERLGVQGNSGEDMLVDLRDRLLALGDMPSLP
                     TGWHAESAIRSAYGDKFSGDLAVTSLSPDGHRLEVALVDLSGKGRTAGTRSLLCSGAI
                     GGLLGQVEPERLLPAANHYLVRQEWPEGFATAVHLAVDLRTGDFTLGNAGHPAPIQFT
                     AGSGRWSVLESAGGPVLGIIPEIQFPRSAGRLGRGDALVLYTDGVIEARGHDLSRGLD
                     RLLGNAERLITSGFAGGARRLCADAQAGDSDDRAVVVIWRD"
     assembly_gap    4569..4668
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4669..4881)
                     /locus_tag="PLB86_07960"
     CDS             complement(4669..4881)
                     /locus_tag="PLB86_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HRC65110.1"
                     /translation="MGLFDEIKDKAEELKDKAVELVQGNSDKVDEAVEKAGDLIDEKT
                     GGQFSDKVDMAQEKAKEVADDLDQAN"
     gene            5007..5678
                     /locus_tag="PLB86_07965"
     CDS             5007..5678
                     /locus_tag="PLB86_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRC65111.1"
                     /translation="MPEQRVRTFHARRGRRSGLTESRLATLLPRYAVPAAVLAPPGTP
                     GRPPHVVLEIGCGHGAAAVGYAATHPDTHLIALDVHPPGVARMLAAAADEGLTNLSAE
                     LADAVEFLADRVPEGGLDAVHLFFPDPWPKVRHHRRRFVSPQTLDLLASRLTPTGQVL
                     VATDIADYAAYVRRVVADHGTFVARDVPRPTWRPLAGYEAKGLDAGRAITELVLERAT
                     RIELA"
     gene            complement(5647..5720)
                     /locus_tag="PLB86_07970"
     tRNA            complement(5647..5720)
                     /locus_tag="PLB86_07970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5686..5688),aa:Gly,seq:tcc)
     gene            5862..5938
                     /locus_tag="PLB86_07975"
     tRNA            5862..5938
                     /locus_tag="PLB86_07975"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5896..5898,aa:Pro,seq:tgg)
     gene            6214..7572
                     /gene="tig"
                     /locus_tag="PLB86_07980"
     CDS             6214..7572
                     /gene="tig"
                     /locus_tag="PLB86_07980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRC65112.1"
                     /translation="MKSALETLSPTRVKLTIEVSAEEFQPSLEQAYKTIGSQIQVPGF
                     RRGKIPARIIDQRLGKGAVLQEAVNDALPTFYGQALEEHSVRPLGQPEVDVTEVPLDA
                     GQGLTFTAEVDKRPEIELPDYSTLAVEVDAIEVGDADIEERLTTLRQRFGTLKGVERA
                     AATGDFVSIDLSAQIDGEEIDTVTGVSYEVGAGNMLEGMDEALVGMTAGETKTFEAPL
                     AGGDHAGAAAECTVTVQSVKERELPELDDEFAQLASEFDTLEELKADLVKDAERGKRF
                     GQGMQARDKVMEALLAAVEIPIPEGVVNDEVHSHLEGEGRLEDDEHRAEVDAETRKGL
                     AAQFLLDAIVEKEEIRVEQPELIEYLLMSAQQYGMDPNQFAQAIDAQGQIPAMVSEVA
                     RRKALAKVLEQASVTDSNGAAVDLEAVMAPRAPFGDDHEGHDHEGHDHEGHDHDGHDH
                     EH"
     gene            7633..8925
                     /locus_tag="PLB86_07985"
     CDS             7633..8925
                     /locus_tag="PLB86_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC65113.1"
                     /translation="MTFTEPSTPREPSAPDAPHVRAAVIAVYAVFLLAGVQFAAFAAR
                     IPQVKDLLGLSAGELGVTLLAVSAGSILGLPAAGWVVHRFGAARTVLAGVIASVLALA
                     TVGATTEILHSRPLTMAALFFAGLGIGIWDMSMNLEGATVERAAGRAIMPRFHAAFSA
                     GTVFSALVGSGLTALGVGVLPHLAGTSLVVLLAALWATPRFLTTGAGAHGIPDAADST
                     GTEPSTSGLGMRAAWTERRTLLIGLVVLVAAFTEGSANDWLAIAFVDGHHLPAWAGVL
                     AFATFLASMTVGRIAGTKLLDRYGRVRVLRATFLTAVLGSVLVVFGGPWLAFLGAALW
                     GVGASLGFPVGMSAGADDPARAAARVSVISTIGYGAFLGGPPLLGFLGDHIGVLRAML
                     VVGLAVLLAFAALPAVREPAPEEDFPQISPQVGFPDGS"
     gene            8966..9415
                     /locus_tag="PLB86_07990"
     CDS             8966..9415
                     /locus_tag="PLB86_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRC65114.1"
                     /translation="MDADIASYLDKVKQRRSELGQSMAELQEVIDEPVGDAEHWRDVA
                     RAAAADVRRDLDEHIAVTEANGGLYDSLRAEAARLAGPLRHLQGEHPDLVALADELIA
                     VLDRPAAADRADAPELVKKLLRRLSHHRQRGADLVFEAYQVDVGGTG"
     gene            9561..>9802
                     /locus_tag="PLB86_07995"
     CDS             9561..>9802
                     /locus_tag="PLB86_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRC65115.1"
                     /translation="MAGAPMPGSLDDHIYNRLLKERIIFLGSEVRDENANAICAQMLL
                     LAAEDPEKDIWLYINSPGGSVTAGMAIFDTMQWVPN"
BASE COUNT         1412 a         3374 c         3400 g         1516 t
ORIGIN      
        1 gggtcgcggt ccaactcggc ggcgatgtcg tcctcagagg cgccgccgga agcggcgatc
       61 tgggtgagca gcgcccagcg caggtcctgg tcgaccgaca ggccaggcac gacgacggat
      121 ccgtcgagga tgccctgcag ccgggtgagg tcgtcggtat gccgcgcgag ggcggcatac
      181 gcgacgacga gttgctgctg cttgtcgctg ccggggaccg cggcgtccag cgccgtgcgg
      241 atgacgtcga cgctcttgtc gaccatcgcg ggacgggcgg cttcgggaag gaaccagcgc
      301 agggtggcca gcagcatcga ggggatctgc ttggtggtgc gcaggatggt gcgcaggacg
      361 acggtgtgct cctcgacagg gatgacccgc agcagcacgt cgacgaaatc cgacggggcc
      421 agggcgccgt cgcggagcat ctcccacagc gcgccgagga cgaggctgcg ggcgaggctg
      481 tcatcgaagg cgtcgatgtg cgccatcgcg gtctgcagcg acaacgggtc gagcgcgatc
      541 ttggcgtagg tgaggtcctc gtcgttgggc agcagcagcg cagggcgggg cagcccgacg
      601 agctcaggca gctcggtgcg cgggccgtcg acgtcgacct cgacgtagcc gacgcggtcc
      661 agccgcccgc cgagcaggtc gtagcgcccg atgcccaccc ggtgcggacg cagcgtcggg
      721 tcagagccgg cgaccgtctg cgtcatggca gcagcggtga tgacaccgtt gtcgtcggtc
      781 gtgatgtcta gtgcgagcgt gctggggccg gaggtcttga gccagcgctg ggcccagtcg
      841 gacaggtcgc gcccggagcc ggcctcgaac tcggcgaaga ggtcacccat cgtcgtgttg
      901 ccccaggcat gcttgcggaa gtagctgcgc aagccctcgc ggaaggcgtc gcgcccgacg
      961 taggccacga gctgcttgag gaccgacccg cccttggcgt aggtgatccc gtcgaagttc
     1021 accgtcacgt cgaccaggtc ccgcatgtcc gcatagacgg ggtgggtgtc gctctgctgg
     1081 tcctgctgat aggcccaggt cttctcgtgg ctatggaacg tcgtccacgc gtccggccac
     1141 tcggtcgcct cggcctggca gaccgacgag gcccactcgg cgaacgactc gttgagccac
     1201 aggtcgtccc accacttcat cgtcacgagg tcgccgaacc acatgtgcgc caactcgtgc
     1261 aggatcgtca gcgcccggcg ctcgacgagc ggctcggagg gctgcccgcg gaagacgtag
     1321 gcgtcggtga tcgtcacgca gccggcgttc tccatggccc cggcgttgta ctcggggacg
     1381 aacaactggt cgtacttcgc gaacgggaag gcgccgtcga actccttctc gaagaacaga
     1441 attcccgcct tggtgcagtc gaataggttc ccgtagtcga catactccgt gatcgagcgg
     1501 cgcgcgaaga tccccatggg gatgacggcg tcgcggccct gcacctcatc ccaacgcacc
     1561 gtgtagggcc cggcacacaa cgccgtgatg taggaggaga tccgcggcgt cggctcgaac
     1621 gcccaggtcg cccgaccctc gccggccggg accggctcgg gagccggctg gttggagacg
     1681 acgacccagt ggtcggggac cgtggccgtg aacgcgaagg tggccttgag gtcgggctgc
     1741 tcgaacacgg catacacccg gcggctgtcg gggacctcga actggctgta gaggtagacc
     1801 tcgttgtcga cggggtcgac gaagcggtgc agtccctcgc cgctgttggt gtagcgcccc
     1861 accgcgtcga cgaccaggac gttgtcgggg gcgagcgccg gcagcgcgat cctgctgtcc
     1921 gcaaagacct cggccgggtc gagggcgacg ccgttgaggg tcaccgacgt caccgagtca
     1981 ccgacgaagt cgatccacgt gccgaccccc gctgcgcgcg cagcgaaccg caccgtcgtc
     2041 gacgtgagga acgtcgtggg tccggtggtg aggtcgagca ccacgtcgta actgttgacg
     2101 tggagcaacg cagcgcggga tgcggcttcg tcgcgggtga ggttcgtgcc gggcacaggg
     2161 acgctccttg ggtgggtggc caagctgctg ggtatgccgc gcggccgggt cagcggtccg
     2221 cgccgcggtg gtttgcccag ccatcttccc acgggccggg cagatcactt cagggacgga
     2281 tggcggggga gtgacacaat gcgccctatg accgccagtc ccgaagcgcg ccccacgcgg
     2341 gtccgccgtc agcccgttga gatgtggttc gacccgatgt gtccctgggc ctggatgacc
     2401 tcccgctggc tcatgcaggt cgaaacggtc cgtccggtgc gggtgacctg gtcggtgatg
     2461 agcttggcag tgctcaacga gggtcgcgac ctgcccgagc agtaccgcgc gctcatggag
     2521 cacgcctggg ggccggtgcg ggtcatcatc gccgcgcagg cagcccacgg cgacaaggtc
     2581 atcaagaagc tctacgacgc cctgggcacc cggatccatc tgcaggcccg gaccgactac
     2641 gaccaggtca tcgccgaagc gctcgccgag tgcaagctcc cggccgagtt ggccaaggcg
     2701 gctcaccaga agaagtacga cgcggccctc aagcgcagcc acaagcgggc catctcgctc
     2761 gtcggcaccg acgtcggaac cccggtgatc tcggtggggg gcgtcgcctt cttcgggcct
     2821 gtcgtctccc cggcccccaa gggtgaggcc gccggccgcc tctgggacgg ctgtgtcctg
     2881 gtcgccggga cgccgggctt cttcgagctc aagcgcaccc gctccatcgg ccccatcttc
     2941 gactgaaagc gattccctca catgcgagtt cacctcggcg gcgaccacgc ggcatacgcc
     3001 ttgcagcgcg cgctggttga ccatctgacc gcagccggac acgaggtcat cgaccacgga
     3061 ccgctcgcct acgacgcgat ggacgactac ccggtgttcg tgctgcgggc ggcccaggcc
     3121 gtggctgccg acccgggatc ggcgggcatc gtcctcggcg ggtccggcaa cggcgaggtc
     3181 atggcggcca acaaggtccc cgggatccgc gccgcgtatg ccgcgagcat cgagttggcc
     3241 gagttggccc gcgagcacaa cgacgcccag atcatctcga tgggcggccg gttcaccgac
     3301 atcgagaccg gctgcgcgat cgccgacgcg ttcctcgcga cggacttctc cggtgacgag
     3361 cggcacgccc gccgactggc gatggtgagt cgcttcgagg ccgacgggag cctgccggaa
     3421 ggctgacgcc ccagatgact gcacagacgg cacgtcgcga ggtcgacccg gggtcgacca
     3481 gggtgtcggt gctgcgtgcc gagccgtggc cagcgcgcct gcgggtggtg ctgcaggcga
     3541 ggcagccggt gcgcgcgttc gggctggtcg tcgccggctg ggtcctgctg gcctgcatgg
     3601 tcgcctggcc cctcaacgtc gcctggtcga tgtacgcgcc actcgtcgtc ctcgccgggt
     3661 tgttcctcac ccctggctgg ttcgcggtgg tcagcgcctc gtatgccgtg tgcctgggga
     3721 tctcggcgtt catggtgcgc gggtggtcgg tgctgcaggg attcaccttg ctggcgctcg
     3781 cgctgacgat gaccctcatg gtgatgcgca ctcggtcacg ggagcggctc ggggtgcagg
     3841 gcaacagcgg tgaggacatg ctcgtcgacc tgcgcgatcg gttgctggcg ctgggcgaca
     3901 tgccgtcgct gccgaccggt tggcacgccg agtcggccat ccggtctgcc tacggcgaca
     3961 agttctccgg cgatctggcc gtgacctcgt tgtcgcccga cggtcatcgc ttggaggtcg
     4021 cgctcgtcga cctgtccggg aagggtcgca ccgcgggcac ccggtcgctg ctgtgttcgg
     4081 gagcgatcgg cgggttgctt ggccaggtgg agcctgagcg gctgctgccc gcggccaacc
     4141 actacctcgt gcgccaggag tggccggaag gtttcgcgac cgccgttcac ctggccgtcg
     4201 acctgcgcac gggcgacttc acgctcggca atgccgggca cccggcgccg atccagttca
     4261 ccgcaggctc ggggcgctgg tcggtcctcg agtcggccgg tggcccggtc ctggggatca
     4321 tcccggagat ccaattcccg cgtagcgcag ggcggctcgg ccgcggggac gcgctggtgc
     4381 tctacaccga cggggtcatc gaggcccgcg gtcacgatct gagccggggc ctggaccgtc
     4441 tgttgggcaa tgccgagcga ctgatcacgt ccgggttcgc cgggggcgct cgtcggttgt
     4501 gcgccgacgc gcaggccggt gactccgacg accgagccgt cgtcgtcatc tggcgcgact
     4561 gaccggccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc agttggcctg
     4681 gtccaggtcg tcagcgactt ccttggcctt ctcctgcgcc atgtcgacct tgtccgagaa
     4741 ctgaccaccg gtcttctcgt cgatcaggtc gccagccttc tccacagcct cgtcaacctt
     4801 gtcgctgttg ccctggacaa gctcgacagc cttgtccttg agctcctcag ccttgtcctt
     4861 gatctcgtcg aaaaggccca tgttggccct cctccccact cgttgtcgcc gcccgtgcgg
     4921 acggccttga cccaccttaa gcgagcccgc gccaccggaa gttcccgccg cgtgcctggc
     4981 gagcgcgagc ggcataccct gtgcgcgtgc cagagcagcg ggtccggacc ttccacgcgc
     5041 gacgggggcg ccgctccggc ctcaccgaga gccgattggc caccctgctg ccccggtatg
     5101 ccgtgcccgc cgcagtcctc gcccctcccg gcacgcccgg gcggccccca cacgtcgtcc
     5161 tcgagattgg gtgcggccat ggcgccgccg ccgtcgggta tgccgccacc caccccgaca
     5221 cgcacctcat cgccctcgat gtgcacccgc cgggcgtcgc ccggatgctc gcggcggccg
     5281 ctgacgaagg gctgaccaac ctgtcggcgg agttggccga cgcggtggag ttcctcgccg
     5341 accgggtgcc cgagggtggt ctcgacgcgg tgcacctgtt cttccctgac ccgtggccca
     5401 aggttcggca tcaccggcgc cggttcgtgt cgccgcagac gctcgacctg ttggcctccc
     5461 gcctgacccc gaccgggcag gtcctcgtgg cgaccgacat tgccgactac gcggcatacg
     5521 tgcggcgggt ggtggccgac cacggaacgt tcgtggcgcg ggatgtgccg cggccgacgt
     5581 ggcgaccgct ggccggttac gaggccaagg gcctggacgc cgggcgagcg atcaccgagc
     5641 tggtgctgga gcgggcgacg agaatcgaac tcgcgtagcc agtttggaag actggggctc
     5701 taccattgag ctacgcccgc gtgcggtggc cgtcgcattg tgacggggcc accacggggg
     5761 gctagcctag tcccgcccgc ccggctcgcg cgaaccacca cgtccgcgcg tcccgtagcg
     5821 ggctcggtgc gtcggccacg gtatgccgtg tgcccaccga ccggggtatg gcgcagtttg
     5881 gtagcgcgtc cgctttggga gcggaaggcc gtcggttcga atccggctac cccgaccatc
     5941 tggtccggcg acgcatgggg gcgcccatga agtcgccgtg gctgtcgttg gaacagcagt
     6001 agcgacgact tcatcggcga cttcatgtgt cgtgactgcc gcccgggcca gctcccaggc
     6061 cgtcggccgc tctcggattc ggcccggccg cacacaggcg gctagcatgg accggattgc
     6121 ccgccgtcgt ccgcgccggc cgccacgcct cgtgcacgca cgggcgcgag ctagccggac
     6181 ggcatacctt cctcccgatc tggagtatcc gcagtgaaga gtgcgctcga gaccctcagc
     6241 ccgacccggg tgaagctgac catcgaggtc tccgccgagg agttccagcc cagcctggag
     6301 caggcgtaca agaccatcgg cagccagatc caggtcccgg ggttccggcg cggcaagatc
     6361 ccggcgcgga tcatcgacca gcgcctgggc aagggcgccg tcctgcagga ggccgtcaac
     6421 gacgcgctcc cgaccttcta cgggcaggcc ctcgaggagc actcggtgcg cccgctgggt
     6481 cagcctgagg tcgacgtcac cgaggtgccg ctcgacgccg gacagggcct caccttcacc
     6541 gccgaggtcg acaagcgccc cgagatcgag ctccctgact actccaccct ggccgtcgag
     6601 gtcgacgcga tcgaggtcgg cgacgctgac atcgaagagc gcctcaccac gctgcgccag
     6661 cgtttcggca ccctcaaggg cgtcgagcgc gccgccgcca cgggcgactt cgtgtcgatc
     6721 gacctgtcag ctcagatcga cggcgaggag atcgacaccg tcaccggggt gtcctacgag
     6781 gtcggcgccg gcaacatgct cgaaggcatg gacgaggcgc tcgtcgggat gaccgcgggc
     6841 gagaccaaga cgttcgaggc cccgctcgcg ggcggcgacc acgccggcgc agccgccgag
     6901 tgcaccgtca ccgtccagtc ggtcaaggag cgcgagctcc ccgagctcga cgacgagttc
     6961 gcccagctgg cgagcgagtt cgacaccctg gaggagctca aggccgacct cgtcaaggac
     7021 gccgagcgcg gcaagcggtt cggccagggc atgcaggccc gcgacaaggt gatggaggcc
     7081 ctgctggccg ccgtcgagat cccgatcccc gagggcgtcg tcaacgacga ggtccacagc
     7141 cacctcgagg gcgaggggcg ccttgaggac gacgagcacc gcgccgaggt cgacgccgag
     7201 acccgcaagg gcctggccgc gcagttcctc ctcgacgcca tcgtcgagaa ggaagagatc
     7261 cgcgtcgagc agcccgagct catcgaatac ctgctcatgt cggcccagca gtacggcatg
     7321 gaccccaacc agttcgccca ggccatcgac gcgcagggtc agatccccgc gatggtcagc
     7381 gaggtcgccc ggcgcaaggc cttggccaag gtgcttgagc aggcctcggt gaccgacagc
     7441 aacggtgcgg ccgtcgacct cgaagccgtc atggccccgc gcgcgccgtt cggcgacgac
     7501 cacgagggcc acgaccacga gggtcacgac cacgagggtc acgaccacga cggtcacgac
     7561 cacgagcact gagcccggcg aaccttgccg accgggtcgc agcgctgacc cgggtcacct
     7621 cacttcaccc tcatgacgtt cactgagccg tccactcctc gcgaaccgtc ggcgcccgac
     7681 gcacctcacg tgcgcgccgc cgtcatcgcg gtgtatgccg tgttcctcct tgcgggggtg
     7741 cagttcgcgg cgttcgcggc ccggatcccg caggtcaagg atctgctggg cctgagcgcc
     7801 ggcgagctcg gagtcaccct cctggccgtc tcggcgggct cgatcctcgg gttaccggcc
     7861 gcggggtggg tcgtccatcg gttcggcgcc gcgcgcacgg tccttgccgg agtcatcgcc
     7921 tcggtcctcg cgctggcgac ggtcggtgcc accaccgaga tcctgcactc ccggccgctc
     7981 accatggccg cgctcttctt cgcgggcctg gggatcggca tctgggacat gtcgatgaac
     8041 ctcgaaggcg ccaccgtcga gcgcgcggcg ggacgggcga tcatgccccg cttccacgct
     8101 gcgttcagcg ccggcacggt gttctcggcc ttggtcggca gcggcctcac cgccctgggc
     8161 gttggcgtgc tgccgcacct ggcgggcacc tcgctcgtcg tgctcctcgc cgcgctctgg
     8221 gcgacgccac ggttcctcac cacgggggcg ggggcgcacg gcataccgga tgccgctgac
     8281 tccaccggca cggaaccgtc gacgtcgggg ctggggatgc gtgcggcctg gaccgagcgg
     8341 cggaccctgc tcatcgggct ggtggtcctc gtggcggcct tcaccgaggg cagcgccaac
     8401 gactggctgg caatcgcgtt cgtcgacggc caccaccttc ccgcctgggc cggggtcctc
     8461 gcctttgcga cgttcctggc cagcatgacc gtcggccgga tcgccggcac gaagttgctg
     8521 gaccgttacg gccgagtgcg ggtgctgcgc gcgaccttcc tcaccgccgt cctcggctcg
     8581 gtcctcgtcg tcttcggcgg gccctggctg gcgttcctcg gtgcggccct gtggggtgtc
     8641 ggcgcgagcc tcggcttccc cgtgggcatg tccgccggag ccgatgaccc agcgcgcgca
     8701 gcggcccggg tgagcgtcat cagcaccatc gggtatggcg cgttcctcgg tgggccgccg
     8761 ctgctgggct tcctcggcga ccacatcggg gtgcttcggg ccatgctcgt cgtcgggttg
     8821 gccgtcctgc tggcctttgc cgccctgccg gccgtgcggg agccggctcc ggaggaagac
     8881 ttcccgcaaa tctcaccgca ggttgggttc cccgacggct cctgatgcgt cacgatggga
     8941 gttccctcac gtgcggaggt ggcccatgga cgcggacatc gcgagttacc tggacaaggt
     9001 caaacaacgg cgctcggagc tcgggcagtc gatggccgaa ttgcaggagg tcatcgacga
     9061 acccgtcggt gacgccgagc actggcgcga cgtggcccgc gccgcagcag cggatgtgcg
     9121 tcgcgacctc gacgaacaca tcgccgtgac cgaagcgaac ggcgggctct acgactcgct
     9181 gcgcgcggag gccgcccggc tggccgggcc actgcgtcac ctgcagggcg agcaccccga
     9241 cctcgtcgcc ctggcagacg agttgatcgc cgtcctggat cgacccgctg ccgccgaccg
     9301 ggccgatgcc cccgagctgg tcaagaagct cctgcgccgg ctgtcccacc accgacagcg
     9361 aggcgccgac ttggtgttcg aggcctacca ggtcgacgtc ggagggaccg gctgacccgt
     9421 gccggtgtgc gctcagggcg aacacgcggc ataccgggat tgtcggcgcg accccggacg
     9481 ttagggtcgg gagagcgggc aagccgtccc gcaccgaccg acgcatggag tcacaggtga
     9541 gccagagcaa cgaggtcgtc atggccgggg cgccgatgcc cggcagcctc gacgatcaca
     9601 tctacaaccg gctgctcaag gagcggatca tcttcctggg ctccgaggtc cgggacgaga
     9661 acgccaacgc gatctgcgcg cagatgcttc tccttgcggc cgaagacccc gagaaggaca
     9721 tctggctcta catcaactcc ccgggtgggt cggtgaccgc cggcatggcg atcttcgaca
     9781 ccatgcagtg ggtgcccaat ga
//
LOCUS       DAORPW010000083         9680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1637_length_9680_cov_790.119481, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000083 DAORPW010000000
VERSION     DAORPW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1637_length_9680_cov_790.119481"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..146)
                     /locus_tag="PLB86_08000"
     tRNA            complement(60..146)
                     /locus_tag="PLB86_08000"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:Ser,seq:tga)
     gene            complement(207..1448)
                     /locus_tag="PLB86_08005"
     CDS             complement(207..1448)
                     /locus_tag="PLB86_08005"
                     /inference="COORDINATES: protein motif:HMM:NF017000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HRC65116.1"
                     /translation="MSTSAVSTAEAVAERALGALVGAKVEDGSAVGWPAWALTDPSIP
                     ARMAVDDVTLYNGDAGIAWALAGLGQALDRRELTALAGRAATRVLGNPDALSGGGLLA
                     GRAGGALAAYSIGLPGGAMSSPRRPMTCDLTDGAAGVLLAQVRTGAAPDHDLVRALAR
                     RASAQEVGWAWADAPTESSAGEGSAGEGSAGDGDRGAPAPLLGLAHGASGVALALVES
                     AAVAPEMRPALALAAGGLAWESGWFDPLQGWPDLRGDESTFPVWWCHGAAGMTAVRLR
                     LLELAEQGVDLGMPLSSLRAQAHAGLELCTDHVEAAVVAADAGELPSAGLTLCHGLGG
                     ALDALGYASQVTGDEAHRLLAADALGRVAGVMGEDPMQWPSGTREAGSSSLFLGLAGV
                     AMIAARLAWPDAGVPCPSLLR"
     gene            complement(1445..2431)
                     /locus_tag="PLB86_08010"
     CDS             complement(1445..2431)
                     /locus_tag="PLB86_08010"
                     /inference="COORDINATES: protein motif:HMM:NF037672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector HopA1 family protein"
                     /protein_id="HRC65117.1"
                     /translation="MSAISLAHEAQLARALEVAGAALAGRAPEAVPVDELAAVLYGQW
                     YAVPNAPAALLAAAASERSSRHTLPLAAILRATHPDAEVWESAVVVRSGLHGTVVVRM
                     EAGGIRAVARGDLTPDVTGKVGRAPEPGDRVAVRARAGGLVEGGWWRTWGGGCRAGQA
                     PAGDLTRIYLGPRADLIHLLVPAVLGALRDLDVPWLFKVGSEWPMLARPDGAVLYLPD
                     TAVGLAAGDATPVVAQLVGAVGGLVSGSGPALSVPVAQGISWVQDPGDGSSFGETVCR
                     ALAVAFLSRPELLRPDDGALRSERFAVLAAALGEAGIDPQAPHLRQRPKEAA"
     gene            complement(2431..3450)
                     /locus_tag="PLB86_08015"
     CDS             complement(2431..3450)
                     /locus_tag="PLB86_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRC65118.1"
                     /translation="MTSAPGLFAAAIARGLVSSGEVLTGRVSVTDQSRSNVVRRIDLD
                     GRPVAFVKSRGAAATLDGDDPIGAEVRVLTALAGVAVVPPLIGGPDTDLWTGAVDGIP
                     LYAVHGTAAHLVAVVRAWGSAVAGLHQAVGVDTLVAQGVPMVARPWVLDPDRLPTSMG
                     EPVVGSPLAVLLDAARSAPVRAIASEVAAGWTADALLHGDLGAANVIVEDSAAPLVRF
                     VDLEGAGLGPPEWDLACALDTLGGLVRQWGCPDVVPAFVAGYRAGGGRGMVDPRHLAI
                     RALVSGWQAAAGLLVRGDLAGAGAEARIHLERSRHWMVVWQRGIARSAEPAVGAPETR
                     ERGAA"
     gene            complement(3452..4840)
                     /locus_tag="PLB86_08020"
     CDS             complement(3452..4840)
                     /locus_tag="PLB86_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65119.1"
                     /translation="MDAQQETRALIRPVKESIEDDLLAREGVVGVDIAEKVSDGKPTG
                     QLSIVVFVQKKKPGARIARGQLIPKEIDGVPTDVQELEIELQSAMVKLDDVVGLVDAT
                     AYPSLHGGISMGPRRSVYLTPPEVPAAGNYVFVGTLGAMVRDRASGATMALTNFHVAC
                     PRGWAVGDRMAQPSLVDNAPAATDFGSLTRSVLSENVDGAVVTLDAGKAWSASIETIG
                     NVAGTTAATIGMPVQKRGRTTEHTFGTVRSTDFTVTINYGGDVGSRTLRNQVRIETDT
                     TRSARFSQKGDSGSVIVADDRNVIGLLFAGSTDGQWTFGNPIAAVQDELGVDIIRYEA
                     PPIITRPSVCDPIRTAVIVCNKVTRPALCEPIQTRVVICNKITQPAVCEPIQTRVVIC
                     NKITQPAVCRPTRIPICDLQLTSAACDFPIPPQENPFGDEAAYGEEFLAGYLAALEAV
                     ATEEAADSDGQG"
     gene            5059..6684
                     /locus_tag="PLB86_08025"
     CDS             5059..6684
                     /locus_tag="PLB86_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC65120.1"
                     /translation="MSAIQPPAQPPAATSLPAPSAIPPTPQLSPEVAQRYGARILDPV
                     TGMRRSDGSAPAATVYVGERLLMRTTPGRALDDTVQALNEVTKDFGVRAERIPTPPAK
                     GERALHPEAMAVLQRHWVDGVRLLPVDDQVRMPVDAWPVLAAVASRDAAAAAPLALDH
                     LMTTAPSIGGTPYWLGTPYWLGTPYWLGTPYWLGTGSAAGEYGRPGSGGRTPVSVVLP
                     DPALTAPPVGRRPVVAVPDTGLGNHPWFPAGGAATSEVRWGGILLGINGPAGGGYPPA
                     PQNPLDPVIESDTGHGTFIAGLIRQLCPQAEVLAIPVMRGDGVVPESVLLNTLTLLLA
                     RHVEALATGSTDGLVDVLSLSLGYYHESPADAAMDAPLGALLRDFGDHGVAVIAAAGN
                     SSTLAPMFPAGLSADVTGFAPDSVPLASVGALNPDGETVAFYSNDGDWVSTFRTGSSL
                     VSTFPVDVTASAQASARVDFAGRARTTQDPDDYRGGFAAWSGTSFAGPVLAGEVAAAL
                     ARDADLATVDKAAAVARGRRAMFGQVTEWKGKE"
     gene            6681..7271
                     /locus_tag="PLB86_08030"
     CDS             6681..7271
                     /locus_tag="PLB86_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC65121.1"
                     /translation="MTPSAPPTLADRAAAAFAAYREGDRERMGELVDLLTPILWHTAR
                     GQRLDQATSEDVIQTAWIRLVEGAEAIAEPQAVLAWLITTVRRESWRVLKRDGRSIPV
                     EEFPETEPDGLLAALDPAAEAMLREDQRVLWRAVSSLSERCQALLRIIAFIDRPDYGA
                     VSEALGMPVGSIGPTRGRCLAKLRGQLAADPTWSTP"
     gene            7268..7774
                     /locus_tag="PLB86_08035"
     CDS             7268..7774
                     /locus_tag="PLB86_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRC65122.1"
                     /translation="MTPEDRSSVADGPLDGTDTLVLGAVRELFSRTDPMPSGLTDRIK
                     FELTLAALHAEVAELQELTLTGVRADSASFTPTESVTFTSSTLSLMVSISPAGEGARP
                     GTVRIDGWVTGGPATVELRAGEKSIAAEADTNGRFSVDEVPRGPVRFILRPVDARGRP
                     VITPVVEI"
     gene            7775..>9680
                     /locus_tag="PLB86_08040"
     CDS             7775..>9680
                     /locus_tag="PLB86_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65123.1"
                     /translation="MTDATDTQPRAVAEAESLRRQAVSAIEDYQPDLAASLLDQAWEL
                     LEDLPRACAALPEACETRARIRLAQSWTTFEREGQVAAAPVLADALDLARAQDRLDLV
                     ALCLMQGATMSGRSGDLPGALTLMRQAEAGLTLLPLPDQVRLVLNRGLIAAQVGQLDD
                     ARDDLGRAADLAARAGAPPMEFMARHNRGYVEYLRGDLPAALSLMESADAMDVAVSRS
                     VSLLDQARVLLEAGLIDEAAHSLRRARDLASAEGGGQDLGEIELDLARTLLLLGDPHG
                     SAELAGQARRRFHDRAAAPWRRQAQLVELEAGSAARDHPERTARIAAALAEAASRHGE
                     VHVCRRARLIEADARLDLGEIEPARAAYTLARPLLTSASLPTRLHLRLVAARLATADP
                     GSPTRRATRHLTEAADDLARTQRRVSSLDVRTALAVHSNRLALLDLGIGVEQGSPASL
                     LARTERWRAVSDRVPLVRPPRDSRAADLLTQLRRTREDLRGAPAEAQVDLRSAATDLE
                     RRIRALDWARSDSGTPDWRPARPVSYPAALAAVRAADATLVSYVPHGPDLYAVCLTPR
                     GGRVLRVARSAEVSGATRRLRADVEAVSLPSLGPMQSAVEASLATQLAALDRLLIGPI
                     AANSARSGSRS"
BASE COUNT         1440 a         3728 c         3202 g         1310 t
ORIGIN      
        1 gcgagcgcca gcgagcggag ggcgtggtgg gtcctacggc caccgcgagc gccagcgagc
       61 ggagggcgtg ggattcgaac ccacgatgcc gttgccgaca tagcggtttt caagaccgcc
      121 gcactaggcc actatgcgag ccctccacga gcagcgtagt gaaccgcggc ccgacgaatc
      181 atcgtagacg agcgtcacga ccaacctcac cgcaacagcg acggacaggg cacacccgca
      241 tccggccagg cgagccgggc cgcgatcatc gccacaccgg ccaacccaag gaacagactc
      301 gacgacccag cctccctcgt ccccgacggc cactgcatcg ggtcctcgcc catcacaccg
      361 gccacccgcc ccaacgcatc ggcagccaac agccgatgcg cctcgtcacc ggtgacctgc
      421 gacgcatacc ccagcgcatc aagcgcccca cccaacccgt gacacagggt cagcccggcg
      481 gacggcaact ccccggcgtc ggcagccacc acggccgcct cgacgtgatc ggtgcacaac
      541 tcgagacccg catgagcctg cgcccggagt gagctcagcg gcataccgag atcgacgcct
      601 tgctcagcca actcgagcag acgcagtcgc acagcggtca tgccagcagc cccgtgacac
      661 caccacaccg ggaacgtcga ctcgtcgcca cgcaggtccg gccacccttg caacggatcg
      721 aaccacccac tctcccaagc aagtccaccc gccgcgagag ccaaggcagg gcgcatctcc
      781 ggggccaccg ccgcgctctc gaccagcgcc agcgccacgc cgctagcccc atgggccaac
      841 ccgagcagcg gagccggcgc gccccggtcg ccgtccccgg ccgacccctc ccccgcagaa
      901 ccctcccccg cagaactctc agtcggcgcg tccgcccacg cccagccgac ctcctgagcc
      961 gaagctcgcc gggccaacgc gcgcaccaga tcgtggtcag gcgccgcccc ggtccgcacc
     1021 tgggcgagca acacgcccgc agcgccatcc gtcaggtcac aggtcatcgg acgcctcggc
     1081 gaagacatcg cgccgcccgg caacccgatc gagtatgccg cgagcgcacc ccccgcccgc
     1141 ccggccagca agcctccgcc ggacaacgcg tccgggttgc ccaggacccg cgtggccgcc
     1201 cgtccagcca aggccgtcaa ctctcgccga tccagcgctt gacccaatcc agccagggcc
     1261 caagcgattc cggcgtcgcc gttgtagaga gtgacgtcat ccaccgccat ccgcgcgggg
     1321 atcgacgggt cggtcagcgc ccacgccggc cagcccacgg cggatccgtc ctcgaccttt
     1381 gccccgacga gggcaccgag agcgcgctcg gcgacggctt cggcggtgct gacggcagaa
     1441 gtgctcatgc ggcttccttc ggacgctggc gcaggtgcgg cgcttggggg tcaataccgg
     1501 cctcgcccaa ggcagcagcg aggacggcga agcgttcgga ccgcagcgcc ccgtcgtcag
     1561 gtcggagcaa ctcgggccgt gaaaggaagg ccacggcgag agcgcggcat accgtctcgc
     1621 cgaagcttga cccgtcgcca gggtcttgca cccacgaaat cccttgcgcg acaggcacgc
     1681 tcaatgccgg tcccgagccg ctgacgagac ctccgacagc ccccaccagc tgcgcgacaa
     1741 ctggtgtcgc gtcccctgca gccagcccca ctgctgtgtc cggcaaatat aggaccgcgc
     1801 cgtccggacg cgccagcatc ggccattcgg aacccacctt gaacagccac gggacgtcga
     1861 ggtcgcggag ggctcccagg acggccggga cgagcaggtg gatcaggtcg gcgcgaggcc
     1921 cgaggtagat ccgggtgagg tcacccgcag gcgcttgtcc ggcgcggcag ccgccgcccc
     1981 acgtgcgcca ccaaccgcct tcgaccagac caccggcgcg ggcccgcacg gccacccggt
     2041 cgcccggctc cggtgcccga cccaccttgc cggtcacgtc tggcgtgagg tcgccacgcg
     2101 ccaccgcccg tatgccgccc gcctccatcc gcacgacgac ggtcccgtgc agacctgacc
     2161 ggacgacgac cgccgactcc cacacctcgg cgtccgggtg ggtcgcccga aggatcgcgg
     2221 ccaggggcag cgtgtgtcgt gatgaccgct cggacgcagc agcagccagg agcgccgccg
     2281 gggcgttcgg cacggcatac cactggccgt agagcactgc cgcgagctca tcgaccggga
     2341 cagcctccgg ggcccgcccg gccagcgcgg cgccagcgac ctcgagggcg cgagcgagtt
     2401 gggcctcatg agcgagggag atcgcactca tcaggcggca cctcgctcgc gcgtctcggg
     2461 tgcccccacc gcaggctcgg cagatcgagc aatgcccctc tgccacacca ccatccagtg
     2521 ccgcgaacgc tccagatgga tgcgggcctc ggcgcccgcg ccggccaggt cgccccgcac
     2581 caacaggcct gccgcagcct gccagccgct gaccagggcc cggatcgcca ggtgccgggg
     2641 gtcgaccatg ccgcggccgc cgcccgcgcg atacccagcg acgaacgcgg gcacaacgtc
     2701 agggcaaccc cactgccgca ctagaccgcc gagggtgtcc aacgcgcagg caagatccca
     2761 ctcgggcggc ccgagcccgg caccttcgag atcgacgaac cgcaccagcg gcgctgcgct
     2821 gtcctcgacg atcacgttcg cggccccgag gtcaccgtgc agcagggcat cggccgtcca
     2881 gccggccgcg acctccgacg cgatcgcccg cacgggcgca ctgcgcgccg cgtcgagcag
     2941 gaccgccagg ggcgagccga ccaccggctc acccatcgag gtcgggagcc gatccggatc
     3001 caagacccac ggcctcgcga ccatcggaac tccttgagcg acaagggtgt cgaccccgac
     3061 cgcctgatgg agtccggcca ctgcggaccc ccacgcccgc acgaccgcca ccaggtgcgc
     3121 ggcggtgccg tgaacggcat acaggggtat gccgtccacg gcaccggtcc acaggtcggt
     3181 gtctggtccg ccgatgaggg gcggcacgac ggccaccccg gccagcgccg tcaacacgcg
     3241 cacctcggcc ccgatggggt cgtcgccgtc gagggtggct gcggcgcccc gtgacttgac
     3301 gaaggcgacc ggccgaccgt cgaggtcgat ccggcgcacg acgttgctcc gggattggtc
     3361 ggtcaccgac acgcgaccgg tcagcacctc acctgaggac accagcccgc gcgcgatggc
     3421 ggcggcgaac aaccccggag cgctcgtcac gtcagccctg cccgtccgag tcggccgcct
     3481 cttcggtggc gacggcttcc agcgcagcga ggtagcccgc gaggaactcc tccccgtatg
     3541 ccgcctcgtc accgaacgga ttctcctgcg gcgggatcgg gaagtcgcac gcggcactcg
     3601 tcaactgcag gtcgcagatc gggatgcggg tcggccggca gacggccggc tgggtgatct
     3661 tgttgcagat gacgacgcgg gtctggatcg gctcacaaac cgccggttgg gtgatcttgt
     3721 tgcagatgac gacgcgggtc tggatcggct cacatagagc cggtcgggtg accttgttgc
     3781 agacgatgac tgccgtgcgg atcgggtcgc acaccgacgg tcgggtgatg atgggcggtg
     3841 cctcgtaccg gatgatgtcg acgcccaact cgtcctgaac cgcggcgatc gggttgccaa
     3901 aggtccactg accgtcggtg gaaccggcga acagcaggcc gatgacattg cggtcgtccg
     3961 cgacgatgac cgaacccgag tcacccttct gggagaaccg cgccgaccga gtcgtgtcgg
     4021 tctcgatgcg cacctggttg cgcagggtgc gggagccgac gtcgccgccg tagttgatcg
     4081 tcactgtgaa gtcggtcgat cggactgtcc cgaaggtgtg ctccgtcgtg cggccccgct
     4141 tctggaccgg cataccgatc gtcgcggccg tggtcccggc gacgttgccg atggtctcga
     4201 tcgacgcaga ccacgccttg cccgcatcga gcgtgacgac ggccccgtcg acgttctcgg
     4261 acaacaccga cctggtgagc gagccgaagt cggtcgctgc gggcgcgttg tccaccaggg
     4321 agggctgggc catccggtct ccgaccgccc agccccgcgg acaggccacg tggaagttcg
     4381 tcaaggccat cgtcgctccg ctggcccggt cccgaaccat ggccccgagg gtgccgacga
     4441 agacgtagtt gcccgccgcc ggcacttcgg gcggggtgag atagacactg cgccgcgggc
     4501 ccatgctgat cccgccgtgc aggctcggat aggcggtggc gtcgaccagg cccacgacat
     4561 cgtcgagctt gaccatcgcc gactggagct cgatctcgag ctcctggaca tccgtgggca
     4621 cgccgtcgat ctccttgggg atgagctgac ccctggcgat ccgggctccc ggcttcttct
     4681 tctggacgaa gacgacgatg gacagttgcc ccgtgggttt gccgtcgctg accttctcgg
     4741 cgatgtcgac accgaccacg ccctcgcgcg cgagtaggtc gtcctcgatc gactccttca
     4801 ccgggcggat caacgcccgg gtctcctgct gtgcatccat gtctggtgct ccttgagctg
     4861 gggagagagt ggtcaccccg aacgggctga cacgtccgga agagcccccg ccgacgcccc
     4921 agatacccgc tggcgccgat tggctcatgt gaggggacct gtccggtatc tggcacggtg
     4981 cccccgactc ctcaccgatg agcaccccga cccctaggct cggagatgag gccacgcatc
     5041 tcccgtgagg acctgaccat gagcgccatc cagccccccg cccagccccc agcggcgacg
     5101 tcgctgcctg cgccgtccgc gatcccgccg acccctcagc tgtcgcccga ggtggcccag
     5161 cgctacggcg cgcggatcct cgacccggtg acggggatgc gtcgcagcga cggcagcgcc
     5221 ccggcggcca ccgtctatgt cggcgaacga ctcctcatgc gcaccacccc tggccgggcc
     5281 ctcgatgaca cggtgcaggc gctcaacgag gtcaccaagg atttcggggt ccgcgccgag
     5341 cgcatcccca ccccgccggc gaagggtgag cgtgccctgc acccggaggc gatggcagtg
     5401 ctgcaacggc attgggtcga cggcgtgcga ctcctccccg tggatgacca ggtccgtatg
     5461 ccggtcgacg cgtggccggt gctggcggcc gtggccagcc gcgacgctgc agccgcagcc
     5521 ccgctggccc tcgatcacct catgacgacc gctccctcga tcggcggcac cccgtattgg
     5581 ctcggcacgc cctactggct cggcaccccc tattggctgg ggacccctta ctggctcggt
     5641 accggttcgg ctgccggcga atacggccgg cccggttcgg gcggacgcac cccggtcagc
     5701 gtggtgctcc ccgacccggc tttgactgca ccgcccgtcg ggcgtcgccc cgtcgtcgcc
     5761 gtccctgaca ccgggttggg caaccacccg tggttccccg cgggcggcgc cgccacgagc
     5821 gaggtgcgtt ggggcggcat actcctcggg atcaacgggc ctgcgggcgg tggctacccg
     5881 cccgccccgc agaaccccct cgacccggtc atcgagagcg acaccggcca cggcaccttc
     5941 atcgccgggc tgatccgcca gttgtgcccg caggccgagg ttctcgctat tccggtgatg
     6001 cgcggcgacg gggtggtccc cgagagcgtg ctgctcaaca ccctgaccct gctgttggcg
     6061 cggcatgtcg aggccctggc caccggctcg accgacggcc tggtcgacgt gttgtcgctc
     6121 tcgctcggtt actaccacga gagcccggcc gacgctgcga tggatgcccc gctgggggcg
     6181 ctgttgcgcg acttcggcga ccacggcgtc gcggtgatcg ccgcagcggg caactcctcg
     6241 accctggccc cgatgttccc cgccgggctg tccgccgacg tcaccggttt cgcgcctgac
     6301 agcgtgccgc tggccagcgt cggcgccctc aaccccgacg gcgagactgt ggccttctac
     6361 tccaacgacg gagactgggt gtcgaccttc cgcaccggga gctcgctggt cagcaccttc
     6421 cccgtcgacg tcacggcatc cgcgcaggcc tcggctcggg tcgacttcgc cggtcgggcg
     6481 cgcacgaccc aggaccccga cgactaccgg ggcggcttcg cggcctggag cggcaccagc
     6541 ttcgccgggc cggtcctcgc cggcgaggtc gcggccgccc tggcccgcga cgccgacctc
     6601 gccaccgtcg acaaggccgc cgctgtggcc cgaggtcggc gcgccatgtt cggtcaggtc
     6661 accgagtgga agggcaagga atgacaccct ccgcaccacc caccttggcc gaccgcgcgg
     6721 ccgccgcgtt cgcggcatac cgcgaggggg atcgcgagcg catgggtgag ttggtcgacc
     6781 tgctcacccc gatcctctgg cacaccgcac gcggccagcg gctggaccaa gccacctccg
     6841 aagacgtcat ccagaccgcg tggatccgcc tcgtcgaagg tgccgaagcg atcgccgagc
     6901 cacaggccgt gctggcctgg ctcatcacga cggtccggcg cgagtcgtgg cgagtcctca
     6961 agcgcgacgg gcgctccatc cctgtggagg agttccccga gaccgagccg gacgggctgc
     7021 tcgccgcact cgaccccgcc gccgaggcga tgctgcgcga ggaccagcgc gtcctgtggc
     7081 gcgcggtcag ctcgctgtcg gaacgctgcc aggcgctcct gcggatcatc gcctttatcg
     7141 accgccccga ttacggggcc gtctcggaag ccctgggtat gccggtcggc tcgatcggcc
     7201 ccacccgcgg gcgctgtctc gccaaactgc gcggccaact ggctgccgac cccacctgga
     7261 gcaccccatg acccccgagg accgcagcag cgtggccgac ggtcccctcg acggcacgga
     7321 caccctggtg ctcggcgctg tgcgtgagct gttcagccgc accgatccga tgcccagcgg
     7381 cctcaccgac cggatcaagt tcgagctcac cctcgctgcg ctgcatgccg aggtagctga
     7441 gttgcaggag ttgacgctca cgggggttcg cgccgacagt gcgtcgttca cacccaccga
     7501 gtcggtgacc ttcaccagtt ccacgctcag cctcatggtg tcgattagcc cggccggtga
     7561 gggcgctcgg ccgggcacgg ttcggatcga cggctgggtc accggggggc ccgcgacggt
     7621 cgaactgcgg gctggcgaga agtcgatcgc ggccgaagcc gataccaacg gcaggttctc
     7681 ggtcgacgag gttccacgcg ggccggtgcg gttcatcctg cgacccgttg atgccagggg
     7741 acggccggtc atcacccctg tcgtcgagat ctaagtgaca gacgccaccg acacccaacc
     7801 gcgggccgtc gccgaggccg agagcctgcg gcgtcaggcc gtctcggcta tcgaggacta
     7861 tcagcccgat ctggccgcca gcttgctgga ccaggcgtgg gaactccttg aggaccttcc
     7921 ccgggcgtgt gcggcgttgc cggaggcgtg cgagacccga gcccggatca ggctggcgca
     7981 gagctggacg acgttcgagc gggagggcca ggtggccgcc gcccccgtcc tcgccgacgc
     8041 tctcgacctc gcgcgggcgc aggaccggct cgatctggtc gccctctgcc tcatgcaggg
     8101 ggccacgatg agcgggcgca gcggcgacct gcccggtgcc ctcaccctca tgcgccaggc
     8161 cgaggccggc ctcaccctgc tgcccctccc cgaccaggtg cgcctcgtcc tcaaccgcgg
     8221 cctcatcgcc gctcaggtgg gccaactcga cgacgctcgc gacgacctgg gccgggccgc
     8281 cgacctggcc gcgcgcgctg gggctccgcc gatggagttc atggcgcggc acaaccgggg
     8341 ctacgtcgaa tacctgcgcg gcgacctccc cgcggccctg tccctcatgg agtcggccga
     8401 cgcgatggac gtggccgtca gccgcagcgt ctccctgctc gatcaggccc gagtgctcct
     8461 ggaagccggg ctgatcgatg aggccgccca ctcgctgcgc cgggcccgcg acctggccag
     8521 cgccgagggc ggcggccagg acctcggcga gatcgagctc gacctcgctc gcacgctgct
     8581 gctgctcggc gacccgcacg gctcggccga gttggccggt caggcccgcc gccggttcca
     8641 cgaccgcgct gctgcgccgt ggcgccgcca ggcccaactc gtcgagttgg aggccgggtc
     8701 cgccgcccgc gatcaccccg agcgcaccgc ccgcatcgcc gccgccctcg ccgaagccgc
     8761 gtcccggcac ggggaggttc acgtatgccg ccgtgcccgg ctcatcgagg ccgacgcacg
     8821 gttggatctc ggcgagatcg agccggcgcg ggcggcatac accttggctc ggccgctgct
     8881 cacctcggcg tcgcttccga cgcgactgca cttgcgtctc gtcgccgcgc ggctggcgac
     8941 tgctgatccc ggcagcccga cgaggcgggc gacccggcac ctgactgagg ccgccgacga
     9001 cctggcccgc acccagcgac gggtctccag tctcgacgtg cgcaccgcgc tagccgtcca
     9061 cagcaaccgc ctggccctgc tcgatctcgg catcggggtc gaacagggct ctcccgcttc
     9121 gctgttggcc cggaccgagc gatggcgggc cgtgtccgac cgggtcccgt tggtccgccc
     9181 gccccgagac tcccgggccg ccgacctcct cacccaactg cgtcgcaccc gcgaagacct
     9241 gcgcggcgca cctgccgagg ctcaggtgga cctgcgctcg gccgccaccg acctggagcg
     9301 ccggatccgg gcgctcgact gggcccgcag cgactccggc acgcccgact ggagacccgc
     9361 gcgtccggtc agctatccgg ccgcgctcgc cgcggtgcgc gccgctgacg ccactctcgt
     9421 cagctacgtc ccccacggcc ccgacctgta tgccgtgtgc ctcacccccc gcggcggccg
     9481 cgtgctgcgg gtggccaggt cggccgaggt gagcggagcc acccgacggc tgcgggccga
     9541 cgtcgaagcc gtctcactgc cgagccttgg ccccatgcag tcggccgtcg aggcgtcgct
     9601 ggccacccaa ctcgccgcgc tcgaccgcct gctgatcggc ccgatcgccg ccaactccgc
     9661 tcgctccggg tcgcgctccg
//
LOCUS       DAORPW010000084         9408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1702_length_9408_cov_753.851278, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000084 DAORPW010000000
VERSION     DAORPW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1702_length_9408_cov_753.851278"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2621)
                     /locus_tag="PLB86_08045"
     CDS             complement(<1..2621)
                     /locus_tag="PLB86_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRC65124.1"
                     /translation="MPTWTMAFGDPGLDLPTGGGKAVNLGRMTRSGFPVPDGFVVTTT
                     AYRHAVAATAGLADLIDAALAEGDPTDPATAESASRAIRAAFRAAPVPAEVAEAVRAA
                     YLALDEGPVAVRSSATAEDLPDLSFAGQQDTYLGIVGEAAVLDAIRDCWSSLWTARAI
                     VYRRRNLVPDAAAALAVVVQRLVPADVSGVLFTANPLTGHRGQMVLDATFGLGEALVS
                     GQVEPDHVVADAASGRVLSRTVGAKAVATVVSPTGGVTTETRQASGESAVTDSQVAAL
                     VALGRRVQAEYAAPQDIEWAIHDGTIHLLQTRAITSLYPVPDGAPPDSVWLSFGAVQG
                     MLAPMTPLGRDAIRCILSGGASVFGHTVDERTNPYLGVAGERLWIRLDRLLRNPLGRR
                     GAPEFLRFVDPSAAAIADTLLGEHWLRPLPRATSRRVAAQTARDLARFGRVAVPRIAR
                     ALARPLPARDRFDDDIDDLIATAREREADAAAQAEPVARLAARARALRADLRGAFPTL
                     LPDAAPLVAPAVVMLRVLSELTDATTDGHGVSPLAMEVTRAIPRNPTTEMDLTLWAVA
                     EAIRADPEATFAFQALTPTELAARYAARRLPDGAQAALDAFLTAYGMRGVGEIDLGRP
                     RWREDPTDLLQTLTVYLDLPEDAAPPAVFRRGEQAAAVAVERLAAVASGMPRGHLRAR
                     TVRWLASRIRALAGGREQPKFAVVQVMGVIREGLLASGADLVALGVLDRPDDVFFLHL
                     SELTGLTQASLGGLRPAVEARRAAWSTEIRRARVPRVLVGDGRAFHEGVARTADLADL
                     PPGTLVGSPVSPGVVEGLVRVVHDPAPSGLQPGEILVCLGTDPAWTPLFLTAGGLITE
                     VGGMMTHGSVVAREYGIP"
     gene            2716..3555
                     /locus_tag="PLB86_08050"
     CDS             2716..3555
                     /locus_tag="PLB86_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HRC65125.1"
                     /translation="MSVRVRLDLGYDGTDFAGWAAQPGQRTVEGELSRALTTLLRAPE
                     PVRLTVAGRTDAGVHARGQVVHADVDEAAYLAVVGRSRRSPEEAAVVRLRGLLGSDIL
                     VHRVCRAPAGFDARFAAVWRRYSYRIADSLATADPVRRRDTVWLPDPVDVDVMNQASA
                     GLLGLKDFAAFCKRRVGATTVRTLLDFTWARGADGVVEARVVADAFCHSMVRSLVGSV
                     VPVGLGREDAAWPGQVQAAAARNGRVKVMPAHGLCLEEVGYPADAALADRARESRARR
                     DGA"
     gene            3587..4954
                     /locus_tag="PLB86_08055"
     CDS             3587..4954
                     /locus_tag="PLB86_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC65126.1"
                     /translation="MTSEVLLPPGVERGPATVRAFRFVLLNTLIANVTTSYLWFALTF
                     WAYLETRSVLATSIIGGSFMLLIAVFGTVFGSVVDRHVKKNVMIGSNLVTLVTFVAAG
                     GLFLAFPTERLIDWNGPVFWVFVLVILVGGVVESMRNIALSTTVTLLIPADDRDKANG
                     FVGTVQGLAFMVTSVFSGLSIGLLGMGWTLVIAIGLTALSLGHLLTIPIPEVPPTRHE
                     SDSAGHFDVRSALEAIRAVPGLMALVLFATFNNLVGGVFMALMDPYGLTLFSVESWGI
                     VLGVTSIGFIIGGAWVAKFGLGATPLRSLMLANVVAAVLGMAFTIREVWWLYAGGILL
                     YMCVIPIAEAAEQTILQRVVPYRTQGRVFGFAQSVEAAASPVSAFLIGPIAQFWLIPY
                     MASDAGKERFGWLLGAGEARGIALVFLLAGAIMLVTVLLALASRPYRQLSRAYASAPP
                     SEASA"
     gene            5024..>6531
                     /locus_tag="PLB86_08060"
     CDS             5024..>6531
                     /locus_tag="PLB86_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC65127.1"
                     /translation="MGHVDVNQVSFDLPDGRPLLREVSLRVGEGARIALVGPNGSGKT
                     TLARIVSGDLAAHSGAVTRSGGLGVMRQFIGSVRDESTVRDLLLSVASDKVRAAAAEI
                     DRTELAVMEHDTERDQLAYAQALVDWGDAGGYEAETLWDTCTVAALGVPYERAQWRPV
                     ATLSGGEQKRVVLEALLRGPEEVLLLDEPDNYLDVPGKRWLEDALIASPKTVLFISHD
                     RELLARVATRVATLEPGAAGSTLWVHPGPFGTYAAAREDRNSRLEELRRRWDEEHIKL
                     KALVVMYRQKAAYNSDMAPKLQAAETRLRRFVEAGPPEAVPVTQRLRMRLTGGRTAKR
                     AIVCERLELTGLMQPFDSEIWFGERLAVLGSNGSGKSHFLRLLACGGSDPDVEHQPVG
                     ETRPEPVRHTGSARLGARVRPGWFAQTHHQPALLGRTLAEILHRGDEHRDGRGREEAA
                     KVLDRYGLAKAAEQTFESLSGGQQARFQILLLELSGATMLLLDEPTDNLDLDS"
     assembly_gap    6532..6631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6632..7572)
                     /locus_tag="PLB86_08065"
     CDS             complement(<6632..7572)
                     /locus_tag="PLB86_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC65128.1"
                     /translation="MADTAVQTAPHRVVGLLVGEVVMFDLAIADQVFGRTDGYTWSVC
                     GVRRGWLPTTTGLAVRVDQGLAALDEADTIVVPGIGDEAWPVPAAALRRLRAAHARGA
                     RVISVCTGAFVLAAAGLLDGRRATTHWRYAGLLASSFPAVEVDPAVLYVDHGTIATSA
                     GLAAGIDLCLHLVARDLGADRAAAVARNMVVPPHRRGGQAQFVEALSATHEGTGLAEV
                     LDFMTVRCAEPLTLADLARRAAMSPRTFTRHFRAQTGVSPKQWLLARRLEAARRLLES
                     SDLSVAEIAVRAGFGTPLALRQQFTRVLRTTPTAYRRT"
     gene            7645..8247
                     /locus_tag="PLB86_08070"
     CDS             7645..8247
                     /locus_tag="PLB86_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRC65129.1"
                     /translation="MNIEILLFDGFDEFDAMAPWEVFAGLAQVTDRVRASFVTLDGAG
                     PVRGDHGAVVMAQGALSERPDLLWVPGGGWLDRSPTGAWGLVQAGEVPRAIAAAHAAG
                     TVVASVCTGALILAAAGVLAGHRATTNPHALDDLRAFAGVTVLEARVVDDGDVVTGGA
                     PACALDVALHLVGRFVGPDIAAAAARELQYVVPAVDAFVG"
     gene            complement(8302..8931)
                     /locus_tag="PLB86_08075"
     CDS             complement(8302..8931)
                     /locus_tag="PLB86_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC65130.1"
                     /translation="MLGFAPLTPDQIRVLAHPLRSRLVDRLRRRGPATATTLAKELDT
                     NSGATSYHLRRLAAYGLVQPAGGGRGRERRWEASARRHTLPGGADAGVGGSGGGASGP
                     IASTRGADPDTESSLDWLDRDYVRHFAEKAELWLDAAPEWPAGWTDPCGLRDGMVLVT
                     APQLADLRAELDGVLARYRRVGQGNPKAKRVAVYTSCQPLDLDSPPLTP"
     gene            9191..>9408
                     /gene="rplM"
                     /locus_tag="PLB86_08080"
     CDS             9191..>9408
                     /gene="rplM"
                     /locus_tag="PLB86_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRC65131.1"
                     /translation="MRTYTPKPGEVTRAWLVIDATDVVLGRLASQTAVLLRGKHKPTF
                     APHVDTGDFVIIINAEKVALTGNKAQLKR"
BASE COUNT         1303 a         3192 c         3428 g         1385 t
ORIGIN      
        1 ggtatgccgt attcgcgggc gacgactgac ccatgcgtca tcatgccgcc cacctcggtg
       61 atcagcccgc cggccgtgag gaacaggggg gtccacgccg ggtcggtccc taggcagacg
      121 aggatctcgc cgggctgcag gcccgacggc gccggatcgt gcaccacccg gaccagcccc
      181 tcgacgacgc cgggggagac cggactgcca acgagggtgc cgggaggcag atcggcgagg
      241 tcggctgtcc gagcgacgcc ctcgtggaac gcccgtccgt caccgacgag cacccgcggc
      301 acccgggccc gtcggatctc ggtgctccac gcggcccgac gggcctcgac cgcggggcgg
      361 aggccaccaa gcgaggcctg ggtgagaccg gtcaactcgc tcaggtgcag gaagaagaca
      421 tcgtcgggcc ggtcgaggac cccgagggcg acgaggtcgg caccgctggc cagcagcccc
      481 tcacggatga cgcccatgac ctgcacgacg gcgaacttcg gctgctcgcg gccgccggcc
      541 agggctcgga tccgcgaggc gagccaccgc acggtgcgcg cgcgaaggtg accacgcggc
      601 ataccggatg ccacggcggc caggcgctcg acggcgaccg ccgccgcctg ctcgccccgc
      661 cggaagaccg cgggcggagc ggcgtcctcg gggaggtcga ggtagaccgt cagcgtctgc
      721 aggaggtcgg tcgggtcctc acgccagcgc ggtcgcccga ggtcgatctc gcccaccccg
      781 cgcatcccgt atgccgtgag gaaggcatcg agggcggcct gggcgccgtc ggggaggcgg
      841 cgggcggcat accgagcggc gagttcggtc ggggtgagcg cttggaaggc gaaggttgct
      901 tcggggtcgg cgcggatcgc ctcggccacc gcccacagcg tgaggtccat ctcggtcgtg
      961 gggttgcggg ggatggcccg ggtgacttcc atggccaggg gcgacacccc gtgcccatcg
     1021 gtcgtggcat cggtgagttc ggagagcacc cgcagcatga cgacggccgg agccacgagc
     1081 ggcgccgcgt cgggcaacag ggtcgggaac gcaccccgca ggtcggctcg aagggcccgg
     1141 gcgcgggctg ccagccgggc caccggctcg gcctgcgccg ccgcatcggc ttcgcgttcg
     1201 cgagcggtgg cgatcaggtc gtcaatgtca tcgtcgaagc ggtcgcgcgc cgggagcggt
     1261 cgagcgaggg ctcgggcgat ccgcggcact gccactcgac cgaaccgggc caggtctcgc
     1321 gcggtctgcg cggcgacgcg ccgggaggtc gctcggggca gggggcgcag ccagtgctcg
     1381 ccaagcaacg tgtcggcgat tgctgcggcg ctgggatcga cgaaccgcag gaactccggg
     1441 gcgccgcgtc ggccgagggg gttgcgcagc agccgatcca gccgaatcca caaccgttcg
     1501 cccgccaccc cgagataggg gttggtccgc tcgtcgacgg tgtgcccgaa gacgctcgca
     1561 cctccggaga ggatgcaccg gatggcgtcg cgaccgaggg gagtcatcgg ggcgagcatg
     1621 ccttggacgg caccgaagga cagccacacc gagtccggtg gcgccccgtc cgggacgggg
     1681 tagagcgagg tgatcgcccg ggtctgcagg agatggatcg tcccgtcgtg gatggcccac
     1741 tcgatgtctt ggggagcggc atactccgcc tgcacccggc gcccgagcgc gacgagggcg
     1801 gcgacctggc tgtcggtgac ggcggattct ccggaggcct ggcgggtctc ggtggtgaca
     1861 ccaccggtgg gcgagacgac ggtggcgacc gccttggcgc cgacggtgcg gctgagcact
     1921 cgcccgctgg cggcatcggc gaccacgtgg tcgggctcga cctggccgga cacgagcgcc
     1981 tcgccgaggc cgaaggtcgc gtcgagaacc atctggccgc ggtgcccggt gagcgggttg
     2041 gcggtgaaca ggacgccgga gacgtcggcg gggacgagtc gctggacgac gaccgccagg
     2101 gcggcggcgg catcggggac caggttgcgg cgtcgataga cgatggctcg cgcggtccac
     2161 aagctggacc agcagtctcg aatggcgtcg aggaccgcgg cctcgccgac gatcccgagg
     2221 taggtgtcct gctgaccggc gaaggacagg tccggcagat cctcggccgt cgcggaggac
     2281 cgcaccgcca ccggtccctc atcgagggcg aggtatgccg cgcgcaccgc ctcggcgacc
     2341 tccgccggga cgggcgcggc tcggaacgcg gcccggatcg cgcgggaggc cgactcggcc
     2401 gtcgccgggt ctgtggggtc gccctcggcg agggcagcgt cgatgaggtc ggccagtccg
     2461 gcggtcgcgg ccaccgcgtg gcgataggcg gtggtggtga cgacgaatcc gtcgggcacc
     2521 gggaaccctg accgggtcat ccgcccgagg ttgacggcct tgccgccgcc agtgggcaga
     2581 tccagccccg gatcgccgaa tgccatggtc caggtgggca taggtccacg ctagtccgcg
     2641 cacggcatac gcgatgatgg tccgcgtgtg tgcgttgatc tgcctgcggc ggccggcctc
     2701 gggacgtggt gaccgatgag cgtgcgggtg cgcctcgacc tgggctatga cgggaccgac
     2761 ttcgcggggt gggccgccca gcccgggcag cggaccgtcg agggtgagtt gtccagggcc
     2821 ctgaccaccc tgctgcgcgc acccgagccg gttcggctca ccgttgccgg tcgcaccgac
     2881 gcgggggttc acgcccgtgg tcaggtggtg cacgccgacg tggacgaggc ggcatacctc
     2941 gccgtcgtcg ggcgctcacg ccggtcgccg gaggaggcgg cggtggtgcg gctgcgcggg
     3001 ctgctcggct ccgacatcct cgttcatcgg gtatgccgcg cacccgccgg tttcgacgct
     3061 cgcttcgcgg cggtgtggcg gcgctactcg tatcggatcg ccgactcgct cgcgacggcc
     3121 gacccggtcc ggcggcgtga cacggtgtgg ctgcccgatc cggtcgacgt cgacgtgatg
     3181 aaccaggcga gtgctgggct gctggggctc aaggactttg cggcgttctg caaacgccgg
     3241 gtgggcgcga cgacggtgcg gacgctcctg gacttcacgt gggctcgggg agcggacggt
     3301 gtcgtcgagg cccgggtcgt ggcggacgcg ttctgccact ccatggtgcg ctcgctggtg
     3361 gggtcggtcg tgccggtggg gctcggtcgg gaggacgcgg cttggccggg gcaggtgcag
     3421 gcggcggcgg cccgcaacgg ccgcgtcaag gtcatgccgg cgcacggcct ctgcctggag
     3481 gaagtcggct atcccgccga tgcggccctg gccgaccgcg ctcgcgagtc ccgggcccga
     3541 cgcgacggcg cctaggggtg tcagcggctg tcgttagggt gtcccgatga cttcggaagt
     3601 gttgttaccg cccggtgtgg agcgcggccc ggccacggtg cgggcgttcc ggttcgtcct
     3661 gctcaacacc ctgatcgcca acgtgaccac ctcctacctg tggttcgccc tgacgttctg
     3721 ggcttatctg gagaccaggt cggtcctcgc gacgtcgatc atcggcgggt cgttcatgct
     3781 gctcatcgcc gtgttcggga cggtgttcgg gagcgtggtc gaccggcacg tcaagaagaa
     3841 cgtcatgatc ggctccaacc tcgtgacgct cgtgaccttc gtggcggcgg gcgggttgtt
     3901 cctcgccttc cccaccgagc gactcatcga ctggaacggc ccggtcttct gggtcttcgt
     3961 cctcgtgatc ctcgtcggcg gggtggtcga gagcatgcgc aacatcgcgc tgtcgacgac
     4021 ggtcacgctg ctcatcccgg ccgacgaccg cgacaaggcc aacggtttcg tgggcacggt
     4081 ccaagggttg gcgttcatgg tgaccagcgt gttcagtggc ttgtcgatcg gcctgctggg
     4141 catggggtgg acgttggtca tcgcgatcgg gctcaccgcc ctgtccctcg ggcacctcct
     4201 gacaatccct atccctgagg ttccgccgac ccggcacgag agtgactcag cggggcactt
     4261 cgacgtccgc agcgccctcg aggcgatccg cgccgtcccc ggcttgatgg ccctcgtgct
     4321 gttcgcgacg ttcaacaacc tcgtcggcgg cgtgttcatg gcgctgatgg acccctacgg
     4381 gttgaccctg ttctccgtcg agagttgggg catcgtgctg ggggtcacga gcatcggttt
     4441 catcatcggc ggcgcgtggg tcgccaagtt cggactgggc gcgacaccgc tgcgctcgct
     4501 gatgctggcc aacgtcgtgg ccgcagtcct cgggatggcg ttcaccatcc gcgaggtctg
     4561 gtggctctat gccggcggga tcctgctcta catgtgcgtc atcccgatcg ccgaagcggc
     4621 tgagcagacg atcctgcagc gagtcgtgcc ctatcggacc caggggcggg tgttcggctt
     4681 cgcacagagt gtcgaggctg cggccagccc cgtctcggcc ttcctcatcg ggccgatcgc
     4741 ccagttctgg ctcattccct acatggcctc cgatgccggg aaggaacgat tcggctggct
     4801 gctcggcgcg ggcgaggccc gcggcatcgc cctggtgttc ctgctggccg gggcgatcat
     4861 gctggtcacc gtgctgcttg ccctggcgtc ccggccctac cggcaactct ctcgggccta
     4921 cgcgtcggcg ccgccgagtg aggcgtcagc ctgagggtcg gccccccaac gctgacgaaa
     4981 ccgagatgac gggggcgggc ggcatacggg agactgccgc gttgtgggac acgtcgacgt
     5041 caaccaggtg agcttcgacc ttccggacgg aaggccgctg ctgcgggagg tgagcctgcg
     5101 cgtcggcgaa ggcgcccgga tcgcgctggt gggtcccaac gggtctggca agacgactct
     5161 cgcgcgcatc gtcagcggcg acctggccgc gcactccggt gccgtgaccc gctcgggcgg
     5221 gctgggggtg atgcggcagt tcatcggctc ggtgcgggac gagtcgacgg tgcgcgacct
     5281 gctcctctcg gtcgcttccg acaaggtgcg cgccgcggcc gccgagatcg accgcaccga
     5341 gttggccgtc atggagcacg acaccgagcg ggaccaactc gcctacgccc aggcgcttgt
     5401 cgactggggt gacgcagggg gttacgaggc cgagacgctg tgggacacct gcacggtggc
     5461 ggccctcggg gtgccctacg agcgggccca atggcggcct gttgcaacac tgtccggcgg
     5521 cgagcagaag cgagtggtgc tcgaagcgct cctgcgcggc cctgaggagg tgctgctgct
     5581 cgacgagccg gacaactacc tcgacgtgcc cggcaaacgg tggctcgagg acgcgctcat
     5641 cgcctcaccc aagacggtgc tgttcatctc gcacgaccgc gagttgctcg cccgggtcgc
     5701 gacgagggtc gccaccctgg agccgggtgc cgcaggctcg acgttgtggg tgcatccggg
     5761 ccccttcggc acgtatgccg ctgctcgcga ggatcgcaac tcgcgcttgg aagagctgcg
     5821 acggcggtgg gacgaggagc acatcaagct caaggcgctc gtcgtcatgt atcgccagaa
     5881 ggcggcctac aactccgaca tggcgcccaa gttgcaggcc gccgagaccc ggctgcgccg
     5941 gttcgtcgag gccggaccgc cagaggcggt gcccgtcacc caacgactgc ggatgcggct
     6001 gaccggcgga cgcaccgcca agcgggcgat cgtctgcgag cggcttgagc tcaccgggct
     6061 gatgcagccg ttcgactccg agatctggtt cggcgagcgg ttggcggtcc tgggctccaa
     6121 cggctcaggc aagtcgcact tcctgcggtt gctcgcctgc ggtgggtctg accccgacgt
     6181 ggagcaccag cccgtggggg agacgcgacc ggagccggtg cggcataccg gctcggcccg
     6241 gttgggcgcc cgggtgcggc cgggctggtt cgcccagacc caccaccaac ccgcgctgct
     6301 cgggcggacc ctcgccgaga tcctgcatcg cggcgacgaa caccgcgacg ggcgcggccg
     6361 cgaggaggcg gccaaggtgc tggaccgtta cggcctggcc aaggcggcgg agcagacctt
     6421 cgagtcgctc tccggtgggc agcaggcgcg gttccagatc ctgctgctcg agttgtccgg
     6481 cgccaccatg ctgctgctcg acgagccgac cgacaacctc gacctggact cnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtgcggcgg taggccgttg gcgtcgtccg
     6661 cagcacccgg gtgaactgct gccgtagggc gagaggggtc ccgaagcccg cgcggacggc
     6721 gatctcggcg accgacaggt cgcttgactc cagcaggcga cgagccgcct ccagtctgcg
     6781 cgccagcagc cactgcttgg gtgagactcc ggtctgcgct cggaagtgcc gggtaaacgt
     6841 ccgcgggctc atcgcggccc ggcgagccag gtcggccaga gtgagcggct cggcgcaccg
     6901 gacggtcatg aaatccaaca cctcggcgag cccggtgcct tcgtgcgtcg cgctcagtgc
     6961 ctcgacgaac tgcgcctgac ctcctcgccg gtgcggcgga acgaccatgt tcctggccac
     7021 ggccgccgcg cgatcggccc ccagatcccg ggcgaccagg tgcaggcaca ggtcgatccc
     7081 cgccgccaga cccgctgacg tggcgatggt cccgtgatcg acatagagca ccgccgggtc
     7141 cacctcgacc gcagggaaag agctcgccag cagccccgca tagcgccagt gcgtcgttgc
     7201 gcgtcgacca tccagaagcc cggcagccgc gagcacgaag gcaccggtgc agacggagat
     7261 cactcgggcc ccccgggcgt gcgcggcgcg cagcctgcgc agcgccgcag cggggaccgg
     7321 ccaggcctcg tcgccgattc ccgggacgac gatcgtgtcg gcctcgtcga gcgccgccag
     7381 tccttggtcg acgcgcacgg ccagcccggt ggtggtgggc agccacccac gacgcactcc
     7441 gcaaacactc caggtgtacc cgtcggtgcg cccgaacacc tggtcggcga tggccaggtc
     7501 aaacatgacg acctcaccga caagcaggcc gacgacgcgg tgcggcgctg tctggactgc
     7561 agtgtcagcc atggctcaaa actatcgcag aatgtctttc cagccactcc cgagagagca
     7621 tcgaccgggc cgaggatggg gggcatgaac atcgagatcc tgctctttga cggcttcgac
     7681 gagtttgatg caatggcgcc ctgggaggtg tttgccggcc tggcccaggt caccgatcgg
     7741 gtccgtgcct cgttcgtcac gctcgacggc gcaggcccgg tgcgcggcga tcacggagcc
     7801 gtggtcatgg cccagggcgc gctgtctgag cgcccggatc tgctgtgggt tcccggtggc
     7861 ggctggcttg atcgctcgcc gaccggggcg tgggggctcg tgcaggctgg cgaggtgccc
     7921 cgagcgatcg ccgcggccca cgccgcgggg accgttgtcg cctcggtctg cacgggagcg
     7981 ctgatcctgg ccgctgcggg tgtgctcgcc gggcatcggg cgacgaccaa cccccacgcg
     8041 ctcgatgact tgcgggcgtt cgcgggggtg acggttctcg aggcccgcgt cgtcgatgat
     8101 ggagacgtcg tcaccggcgg ggcccccgcg tgtgccctgg acgtcgccct ccacctggtc
     8161 ggtcgattcg tcggcccgga catcgcggcc gccgctgccc gggaactgca gtacgtcgtc
     8221 ccggccgtcg acgccttcgt cggctgatcc gagccacgac ggggccgcct cctgaccctc
     8281 cccctcggac agcgtcgttc cctagggggt caggggcggg gagtccagat ccaggggctg
     8341 gcaggaggtg taaacggcaa cccgcttcgc cttggggttg ccctgcccga cccggcggta
     8401 gcgcgccagc accccgtcga gctcggctcg cagatcggcc agttgtggtg cggtgaccag
     8461 caccatcccg tcgcgcaggc cgcaggggtc ggtccagccc gccggccact ccggggcggc
     8521 gtcgagccag agctcggcct tctcggcgaa gtgccgcacg tagtcgcggt cgagccagtc
     8581 caggctcgac tcggtgtcag ggtcggcgcc gcgggtcgac gcgatcggtc cgctggcacc
     8641 cccgcccgac ccaccaacgc ctgcgtcggc gccgcccggc agggtatgcc gccgcgcgct
     8701 cgcctcccag cgccgctccc gtccgcgccc gcctcccgca ggttggacca gcccgtatgc
     8761 cgcgagccgg cgtaggtgat aactcgtcgc tcccgagttg gtgtcgagct ccttggccag
     8821 ggtggtggcg gtcgccggcc cgcgccgacg cagccggtcg accaggcggg agcgcagggg
     8881 atgggccagg acccggatct ggtcgggggt caatggggcg aaaccgagca tgcacacaag
     8941 ttttgcacac tgtctttgca cactctcacg ccacgccagt cagggcggca tcgtcgcagg
     9001 tcagcggccg attcgcggcg aattgggcgt ggcccgtccg cccggtagac ttgaggactg
     9061 ttgtgccacg cgcggcggta tgccgggtgg gtgccacacc gacccggacc acccgcgagg
     9121 gggagccggc cgcggtgcca ggcacacggc ataccccgaa gctgtgaacc cgaaacggaa
     9181 agtgacgcac atgcgcacct acacccccaa gcccggcgag gtcacccgcg cctggctcgt
     9241 catcgacgcg acggacgtcg tgctcggtcg tctggccagc cagaccgccg tgctgctgcg
     9301 cggcaagcac aagccgacct tcgcgccgca cgtcgacacc ggcgacttcg tcatcatcat
     9361 caacgccgag aaggttgccc tcaccggcaa caaggcccag ctcaagcg
//
LOCUS       DAORPW010000085         9366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1720_length_9366_cov_892.116314, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000085 DAORPW010000000
VERSION     DAORPW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9366
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1720_length_9366_cov_892.116314"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLB86_08085"
     CDS             complement(<1..596)
                     /locus_tag="PLB86_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRC65132.1"
                     /translation="MSDIDQLLTQIPLAAIAQELGVDESQAAASSRTALEALLGGLQA
                     NASDPAGALSLGQALGTHDGSLLGADLGDIDQEDGSAIVHNIFGGNTSDVIAQLGGVK
                     GSGGSGLIAKLLPILAPFVLAWLGQKLRGQGGMGDILGQVLGGAAGGAGGSSSGAGGG
                     GLGDILGQVLGGAAGGAGGSSSGAGGGGLGDILGQVLGG"
     gene            758..1684
                     /locus_tag="PLB86_08090"
     CDS             758..1684
                     /locus_tag="PLB86_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRC65133.1"
                     /translation="MRTVTLPGQSWITDVGPVDGLAMRAWDLSDDLDDPSVRVVVPPM
                     GTTERLARLTALPGLAVVQVFTAGYDHVLAHLPSGVTLCNAAGVHDASTAEFAVALML
                     AAQRGIPEFARAQERGEWIGQRAWPALGDRRVLIVGYGRIGRALAARLAPFEVSLTAV
                     ASRPRAGDDVVERVHGFDELPWLLPHHDIVVLLTPLTDTTRGLADAAFLASMPDGALV
                     VNVARGQVLDTAALLAETATGRLRAALDVTDPEPLPPDHPLWHAPGVLVAPHVGGAGD
                     TFRPRMVRLLREQLTRYAAGAPLLHVVAGPGA"
     gene            1723..2247
                     /locus_tag="PLB86_08095"
     CDS             1723..2247
                     /locus_tag="PLB86_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRC65134.1"
                     /translation="MISDQATKRLLLLRHAKAEQTFGVLDHERELTDRGRRDAHAVGE
                     WLAAQEIDADLVICSTALRTRQTWDHVAAGGARAEAITFDSAIYHGGPTGVLQALVEG
                     AGTAATVVIVGHAPTMPMLAEALTAGRGSAQAHSELAAGFPTTGLAVLRYAGHWADLG
                     QGTAELEAFFIGRG"
     gene            complement(2388..2993)
                     /locus_tag="PLB86_08100"
     CDS             complement(2388..2993)
                     /locus_tag="PLB86_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC65135.1"
                     /translation="MSVATAYRHLHSDAVRLLAGWTPPDARQERLRADYLTHLGAHPD
                     GVAKAGPPAHLTASALVFDSRLERVLLTHHGKARVWLQLGGHLEPEDTSLYAAAQREI
                     REESGLAGVHVIPVIAQLDRHTLVGAFGHCREHLDVRFAAVVPAGSEDAVSASAESID
                     LRWWPLEALPEPTSAEVGALAKACLAVLHAAPSVSPEPSRT"
     gene            complement(2990..4522)
                     /locus_tag="PLB86_08105"
     CDS             complement(2990..4522)
                     /locus_tag="PLB86_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRC65136.1"
                     /translation="MSEPNDDAAAVPDDEASVSSGSQHPAGGPKDGPGATGANQPGGI
                     PDLEPLVRELFGPDATLPPELTELLGALRADPTGSPLAGMLQAQFRSLFAPASTADRV
                     ATATDLARKVVAAKGDPSVGPVQRRAVEEAVAVARLWLDPVTALDSPTGPALALSGAE
                     WVQATTPRWYALVEPVSDGVTAAAGAALRAQVEQLGGLSGGLEGVPELQALLGAIPGG
                     AGLASGAGLWAQIGPALDHLSAGMFGAQLGQAVGTLAGDVLSGTEVGLPLMDSGAVAL
                     LPEQVSEFARGLEVDEGQVRLYLAVREAARVRLFAEVPWLAAQLEAAVRDYGRNVAID
                     TDAIEEAVRSVELTDLSNLSAMQEAFSQAQLFGRRTTPEQDAALIRLEATLALVEGWV
                     DLVTDQAVAAHLPQAHALGEAVRRRRAGGPAQRAFAGLVGLELRPRRLRDARNLWAAL
                     EDAGGVELRDGSWAHPDLAPAAADLDDPLGYVDRRQHPAAPDEMDAELDRLLSGQDPD
                     AL"
     gene            4585..5712
                     /locus_tag="PLB86_08110"
     CDS             4585..5712
                     /locus_tag="PLB86_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRC65137.1"
                     /translation="MSSRQAPRSREVSAGAVRPAGALPRVAVVGAAAVPGARILDTIA
                     RAARASGDGRPPVAIDRDRGRVATAEWRLVDPTDPALVRALRDVDIAVWVAAETDLQS
                     ALRVRPTERRDLVVRTAQTLVTAAAAAGVSRLVVVTSAKVYGATPDNPVPLPEDSDLN
                     GVRDGGVVGDLLAVEEVIQDARRVHPGLSITVVRPAALVGPGIDTVVTRHFEAPRLLT
                     VKGANPAWQFCHVDDLASGVRVVCEQDLGPVVTVGAPGWLSQEQVESLTGMRRVQLPE
                     ATALGTAARLHRFGVLPAPASELAHALYPWVIDSARLRTVGWAAAYDNETCVGAMLES
                     IKGRHAVAGRRVDRMDAALGAASAAVALVGTAAILRRARRP"
     gene            5736..6173
                     /locus_tag="PLB86_08115"
     CDS             5736..6173
                     /locus_tag="PLB86_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRC65138.1"
                     /translation="MDAEVGGAVALARVQDQPLSVDEVLAAVRHDRAGAVALFVGQVR
                     DHDGGRGVVALDYSAHPSADSVAETITQRYAARPGIERVAMVHRVGSLVVGDLAIVAA
                     VSAAHRGEAFEACSQLVEEVKAALPIWKHQQFADGTHEWVGIG"
     gene            6170..7318
                     /locus_tag="PLB86_08120"
     CDS             6170..7318
                     /locus_tag="PLB86_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRC65139.1"
                     /translation="MSGSSSWSGSWTPPEETMPVVESRGARHTRRFWPPWAGLATGMF
                     LAMVLAVLASVIPLPYAILKPGPATDVLASHDGGDGQQTPRIAIDGAPTFATTGSLEF
                     TTVRVEGGPGFPVNAWDLLTAWLSPAQDVYHVDELFPPAATQEQVAEENKAEMAGSQQ
                     EAAAVALRALGQEVPQVVVVRQVVKGAPAEGELQAGDVIVSIGGAAARDSTAIRTAIQ
                     GVTPGDTVEVVVERSGAQVTTHPDTGKADDGRTVLGIVLGVDYKLPFSVKIDVGNVGG
                     PSAGLMFSLGIYDKLTDGALTGGSVIAGTGTIDEKGSVGPIGGIAQKMVGAREGGATF
                     FLAPADNCAEAAGREPSGLQVIKVSSFDDALAAVTAIGKGNTSGLPHC"
     gene            7514..>9366
                     /locus_tag="PLB86_08125"
     CDS             7514..>9366
                     /locus_tag="PLB86_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRC65140.1"
                     /translation="MTVAVLIGLGIVVTAVAQVWTEVMWFDSVNLRSVFTTQLGAQLL
                     LGIGGGLITAALVWSSLHFAHRARPIYPPVTPQQDALDRYREALEPIRRLATLVIPLI
                     AGLATGLTASSQWETFLLWRNAQPFGITDPQFGLDLGFFVFSLPWLTFAVGFVTMAAV
                     LAAIAAAFTHYVYGGLQLANRGRDTTRFALLHLSSLFAVIVLLRAASYWLERYTLSLS
                     TSALMTGIQYTDANAVLPTKAILAVASLMCAAMFLSVIWTRSWRLPVVGTVLLLITSV
                     VVGGIFPALIQSLKVKPSEKSLEAPYIARNIEATRAAYGLGAIEVSKYVPSDEASAEN
                     LRATAAAIPGIRIVDPNVVSPTVRQKHAVRNYYAFPDALDVDRYTIDGKLRDVVVAAR
                     ELDLTGVPAGQRNWLNDHTVYTHGYGIVAAYANQQDSEGNPVYVEAAIPAGTALGEYE
                     PRIYFGEQSPDYAIVGAPEGAAPREFDYPAQVESQQVNNTYAGTGGVPLDNTIRKLAY
                     GLKYRELNFMLSDAVNASSRIMDYRTPKERVGRVAPWLTLDGNAYPAIVDGRVLWIVD
                     GYTTTANYPNSGLTSMAASTRDSTTSTRRSVTSLEQGQLNYIRNSVKATVDA"
BASE COUNT         1338 a         3310 c         3368 g         1350 t
ORIGIN      
        1 ccgccgagga cctgaccgag gatgtcaccg agaccgccgc cgcccgcacc ggacgagctg
       61 ccaccggcgc cgccggccgc tccgccgagg acctggccga ggatgtcacc gagaccgccg
      121 ccacccgcac cggacgagct gccaccggcg ccgccggccg ctccgccgag gacctggccg
      181 aggatgtcgc ccatcccgcc ctgaccgcgc agcttctggc ccagccaggc cagcacgaac
      241 ggggccagga tcggcagcaa cttggcgatg aggccggagc cgccggaccc cttgacgccg
      301 ccgagctggg cgatgacgtc ggaggtgttt cctccgaaga tgttgtggac gatcgccgag
      361 ccgtcctcct ggtcgatgtc cccgaggtcg gcgccgagca ggctgccgtc gtgggtgccg
      421 agcgcctggc cgagggacag cgcgcccgcc gggtcgctcg cgttggcctg cagcccgccg
      481 aggagcgcct caagcgccgt gcgcgaggat gccgcggcct gggactcgtc gacccccagt
      541 tcctgggcga tcgcagccag tggaatctgc gtgagcagtt ggtcgatgtc ggacatgggt
      601 tccctcccgt ggatgcagcc cctgcggccg cctgctcacc ctaacgatcg acgcctgtcg
      661 gcggatcccc cagcgggccc gcaccgaccg tcggcgggtc actcgcgccc gccggtcgcg
      721 ggctggccca tcggcgtccg ggcggtagcg tcggaccgtg cgaaccgtga ccctgcccgg
      781 gcagtcctgg atcaccgacg tggggcccgt ggacgggctg gccatgcggg cctgggactt
      841 gtccgacgac ctcgacgatc cgtcggtgcg cgtcgtcgtg ccgccgatgg gcacgacaga
      901 gcggctggct cgcttgactg cgcttccggg gctcgcggtt gtccaggtgt tcaccgccgg
      961 ctacgaccac gtcttggcgc acctgccgtc gggggtgacc ttgtgcaatg ccgcgggcgt
     1021 ccacgacgcc tccaccgcgg agttcgcggt cgcgctcatg ctcgccgcgc aacgcggcat
     1081 accggaattc gcgcgggctc aggaacgcgg cgagtggatc gggcagcgtg cctggcccgc
     1141 gttgggcgac cggcgggtgc tcatcgtcgg ctacgggcgg atcgggcgcg ccctggccgc
     1201 tcggctcgcg ccgttcgagg tgagcctcac cgcggtggcc tcgcgtccgc gtgccgggga
     1261 cgacgtggtc gagcgggtcc acgggttcga cgagctcccg tggctgctgc cccatcacga
     1321 catcgtcgtg ctgctcacgc cgctcaccga cacaacccga gggcttgcgg atgccgcgtt
     1381 cctcgcgtcg atgcccgacg gcgccctggt cgtcaacgtc gcccgcggcc aggtgctcga
     1441 cactgccgcg cttctggccg agaccgccac gggccggctg cgggctgcgc tggacgtcac
     1501 cgaccccgag ccgctcccgc ccgaccatcc gctgtggcac gccccgggcg tcctcgtcgc
     1561 gccgcacgtc ggcggggcgg gcgacacctt ccggccgcgg atggtgcgtc tgctgcgtga
     1621 gcaactgacc cggtatgccg cgggcgcacc cctgctgcac gtcgtcgccg gaccgggggc
     1681 ctgagggtgg cgagcgcggg ccggaccggg catcctcagg gggtgatcag cgaccaagcg
     1741 accaagcgac tgctgctcct gcgccatgcc aaggccgagc aaaccttcgg tgtcctcgac
     1801 cacgagcggg agctcaccga ccgcggccgc cgggacgccc acgccgtcgg cgagtggctg
     1861 gcagcccagg agatcgacgc cgacctggtc atctgctcca cggctttgcg cacccgccag
     1921 acctgggacc acgtcgcggc gggtggcgcg cgagcggagg cgatcacctt cgactcggcg
     1981 atctatcacg gcggaccgac cggggtgctg caggctctcg tcgaaggcgc gggcaccgct
     2041 gccaccgtcg tcatcgtcgg tcatgccccg acgatgccga tgctcgccga ggcgctcacg
     2101 gccgggcgtg gctcggcgca ggcccattcc gagctcgccg ccggcttccc gacgacgggc
     2161 ctcgccgtgc tgcggtatgc cggtcactgg gccgacttgg gtcagggaac cgccgagttg
     2221 gaggccttct tcatcggtcg cggctgaccg attcgtggac ggcgccgctg cccggggatg
     2281 aagccgctca tgaagccgcg acagctggcg tgatcacgcc agctgtcgcg gcctcatgga
     2341 cggccccagg cccggcacca ctgtccggca cgggtgcggg ccttggatca ggtgcgcgag
     2401 ggctccgggg acaccgacgg cgcagcgtgc agcacggcga ggcaggcctt ggccaaggca
     2461 ccgacctcgg cgctggtggg ctcgggcaac gcctccaggg gccaccaacg caggtcgatg
     2521 gactcggcgc tggctgacac ggcatcctcc gagccggccg gcacgaccgc cgcgaaccgg
     2581 acgtccaaat gctcccggca gtgcccgaag gctccgacga gggtatgccg gtccaattgc
     2641 gcgatcaccg ggatcacgtg gaccccggcc agcccggact cctcgcggat ctcgcgttgc
     2701 gcggcggcat acaggctggt gtcctcaggc tcgagatgac cacccaactg caaccagacc
     2761 cgggccttgc catggtgggt gagcaggacc cgctcgagcc gcgagtcgaa gaccagggcg
     2821 ctcgcggtca ggtgggcagg cggcccggcc ttggcgacgc cgtcggggtg ggcacccagg
     2881 tgggtgaggt agtcggcccg caggcgctcc tgtcgggcat cgggcggggt ccaccccgcc
     2941 agcagacgga ccgcgtcgga gtgcagatgc cggtatgccg tcgcgacgct cacaacgcgt
     3001 ccgggtcctg gcccgacagg agccggtcca actcggcgtc catctcgtcg ggcgcggcgg
     3061 ggtgttgacg gcggtcgacg tagcccagcg ggtcgtcgag gtcggccgcg gcgggggcca
     3121 ggtccgggtg agcccaagac ccatcgcgca actcgacgcc gccggcgtcc tccaatgcgg
     3181 cccacaggtt gcgcgcgtcc cgcaaccgtc ggggccgcaa ctccaggccg accagaccgg
     3241 cgaaggcgcg ctgggcgggc cccccggcgc gacgtcgcct gaccgcctcg ccgagcgcgt
     3301 gagcctgggg caggtgggcg gcgacggctt ggtcggtcac gaggtcgacc cagccctcga
     3361 cgagcgcgag ggtggcctcc aagcggatga gggcggcgtc ctgctcaggg gtcgtgcgtc
     3421 gaccgaagag ctgggcctgc gagaaggcct cctgcatggc cgacaggttg ctcagatcgg
     3481 tgagctcgac ggaccggacg gcctcctcga tcgcgtcggt gtcgatcgcg acgttgcgcc
     3541 cgtagtcgcg taccgctgcc tccaattgag ctgccagcca agggacttcg gcgaagagcc
     3601 ggacccgggc cgcctcacgc acggcgaggt agaggcggac ctgaccctcg tcgacctcca
     3661 gaccacgagc gaactcggag acctgctcag gcagcagcgc gacggccccg ctgtccatga
     3721 gggggagccc gacctcggtg ccggagagga catcaccggc aagcgtgccg accgcctggc
     3781 cgagctgggc gccgaacatt ccggccgaga ggtggtcgag ggcggggccg atctgagccc
     3841 acagcccagc acctgaggcc aggcctgcgc cgccggggat tgctcccagc aacgcctgca
     3901 actcgggaac cccttcgaga cctccggaga gcccacccaa ctgctcgacc tgcgctcgca
     3961 gcgccgcccc ggccgcagcc gtgaccccgt cggagaccgg ttcaacgagg gcgtaccagc
     4021 gcggggtcgt cgcctgcacc cactcggcgc cgctgagcgc cagcgccggt ccggtggggg
     4081 agtcgagcgc ggtgaccggg tcgagccaga gccgggcgac cgccacggcc tcctcgacgg
     4141 cacggcgctg caccggcccg accgacgggt cgcccttggc ggcgaccacc ttgcgggcca
     4201 gatcggtcgc ggtcgcgacc cgatcggccg tcgaggccgg ggcgaagagc gagcggaact
     4261 gagcctggag catgccggcg aggggggatc cggtggggtc ggcgcgcaac gcgccgagca
     4321 actcggtcag ctcgggagga agggtcgcgt cgggaccgaa gagctcgcgc acgaggggtt
     4381 cgagatcagg tatgccgccc ggctggttgg cgccggtcgc cccggggccg tccttcggac
     4441 cgcccgcggg gtgctgcgaa ccggacgaaa cggaggcctc gtcatcgggg acggctgcgg
     4501 cgtcgtcgtt cggctcggac ataaaatcgt gctcctaagc aggggctgag aagatggcac
     4561 ccaagacaac cacaggaggg cggcgtgagt tcccggcagg ctccacggtc tcgggaggtc
     4621 tccgcagggg cggtgcgccc cgcgggtgcc ttaccgcgcg ttgccgtcgt cggtgccgcc
     4681 gccgttccgg gtgcccggat cctcgacacg atcgcccgcg cagcccgagc ctccggcgac
     4741 gggcgtccgc ccgtggccat cgatcgggac cgtggccggg tcgccacggc cgaatggcgt
     4801 ctggtcgatc cgaccgaccc cgccctggtc cgggcgctgc gggacgtcga catcgccgtc
     4861 tgggtggccg ccgagaccga tctgcagagc gccctgcggg tgcgcccgac cgaacgccgg
     4921 gacctcgtcg tgcgcacggc ccagaccttg gtgaccgccg ccgcggcagc cggggtgagc
     4981 cggctggtcg tcgtcacgtc ggccaaggtc tacggcgcca cgcccgacaa cccggtgccg
     5041 ctcccggaag actcggatct caacggggtc cgtgacggtg gcgtcgtcgg tgatctgttg
     5101 gccgtcgagg aggtcatcca ggacgcccgt cgggtccatc ccggcctgtc gatcacggtg
     5161 gtgaggccgg cggccctcgt cgggccgggc atcgacaccg tcgtcacccg acacttcgag
     5221 gcaccccggc tgttgacggt caagggtgcc aatcccgcgt ggcagttctg ccacgtcgat
     5281 gacctggcca gcggcgttcg ggtcgtctgc gagcaagatc tcgggccggt cgtcacggtc
     5341 ggcgctccag gctggttgag ccaggagcag gtcgagtcac tcaccgggat gcgccgggtc
     5401 cagctgcccg aggcgaccgc cctcggcacg gcggcgcgtc tgcaccgctt cggggtgctg
     5461 cccgccccgg ccagcgagtt ggcgcatgcg ctctatccgt gggtcatcga ctcggcgcgg
     5521 ctgcggacgg tcgggtgggc cgcggcatac gacaatgaga cctgcgtggg cgccatgctg
     5581 gagtcgatca agggtcgtca tgcggtcgcc ggtcgccggg tcgaccggat ggacgcggcg
     5641 ttgggtgccg cgagcgcagc cgtcgctctg gtgggcacgg cggcaatcct gcgacgggct
     5701 cgacggccat gacggcgacc gagggaggta gcggcgtgga cgctgaggtg ggcggcgccg
     5761 ttgcgctggc tcgggtgcag gatcagccgt tgtcggtcga cgaggtgctc gctgccgtgc
     5821 ggcacgaccg ggcaggcgcg gtcgccctct tcgtcggcca agtgcgcgac cacgacggtg
     5881 ggcggggcgt cgtcgcgctg gactattcgg cgcatcccag cgctgactcg gtcgccgaaa
     5941 ccatcacgca gcggtatgcc gcccgccccg gtatcgagcg ggtcgcgatg gttcaccggg
     6001 tcggatcgct tgtggtcgga gatctcgcca tcgtcgccgc ggtgagcgcg gcgcatcggg
     6061 gcgaggcgtt cgaggcgtgc tcccaactcg tcgaagaggt caaggcagcg ctcccgatct
     6121 ggaagcatca gcagttcgca gacggaacgc acgaatgggt gggcatcgga tgagcggctc
     6181 aagctcgtgg tcagggtcgt ggaccccgcc cgaggagacg atgcccgtgg tcgagagccg
     6241 gggcgcacgg catacccgac ggttctggcc accctgggcg ggtctggcca ccgggatgtt
     6301 cctggcgatg gtcctcgccg tgctggcctc ggtgatcccc ctgccctacg cgatcctcaa
     6361 accgggcccg gccaccgatg tactggcctc ccacgacggt ggggacgggc agcagacgcc
     6421 gcgaatcgcc atcgatggtg ccccgacctt cgcgacgacc gggtcgttgg agttcacgac
     6481 ggtccgggtc gagggcgggc cgggcttccc ggtcaacgcg tgggacctgt tgacggcctg
     6541 gctgagcccg gcgcaggacg tctatcacgt cgatgagttg ttcccgccgg cggccacgca
     6601 ggagcaggtg gccgaggaga acaaggccga gatggccggc tcgcagcagg aggcggcagc
     6661 ggtcgctctc cgagcgctcg gccaagaggt ccctcaggtc gtcgtcgtgc gccaggtcgt
     6721 caagggggcg ccggccgaag gagagctgca ggccggtgac gtcatcgtgt cgatcggcgg
     6781 ggcggccgcc cgcgactcga ccgcgatccg gacggcgatc caaggggtga cgcccggcga
     6841 caccgtcgag gtcgtcgtcg aacggtcagg ggcgcaggtg accacccatc ccgacaccgg
     6901 caaggccgac gacggccgga cggtgctcgg catcgtgctg ggcgtcgact acaaactgcc
     6961 gttctcggtg aagatcgacg tcggaaacgt cggcgggccg tcggcggggc tgatgttctc
     7021 gttggggatc tacgacaagc tcaccgacgg ggcgctcacc ggtgggtcgg tgatcgccgg
     7081 gaccggcacc atcgacgaga aggggtcggt cgggccgatc ggaggcatcg cccagaagat
     7141 ggtgggagcc cgtgagggcg gcgcgacctt cttcctggcc ccggcggaca actgtgccga
     7201 ggcggcgggc cgcgagccgt ccgggctgca ggtcatcaag gtcagcagtt tcgatgacgc
     7261 actggctgcg gtgaccgcga tcgggaaggg aaatacgtcg gggctgccgc actgctgagc
     7321 cgttcgcgtc gcggtcggcc acggatgttc accagccgtt gccgaatggg tgaatgcgaa
     7381 gcgcgtcgct agcggtaggt agattgacct ggtggatgag gaaaagtggt ttgacggcga
     7441 taccgaccag cccccgaaca ccccgcctcc aggtggccga ccagtagtag cgccccgacg
     7501 cagtccactc ggggtgacgg tcgcggtgct catcggtctc gggatcgtcg tcacggccgt
     7561 ggcccaggtc tggaccgagg tcatgtggtt cgactcggtc aacctgcgct cggtgttcac
     7621 gacgcagctc ggggcccagc tcctgctggg catcggtggc ggcctcatca ccgccgcgct
     7681 ggtctggtca agcctgcact tcgcccaccg ggcccggccc atctacccgc cggtcacgcc
     7741 ccaacaggat gccctcgacc gttaccgcga ggcgctcgag ccgatccggc gactggcgac
     7801 cctcgtcatt ccgctcatcg ccgggctggc caccgggctc actgcgtcgt cgcagtggga
     7861 gaccttcctg ctctggcgca acgcccagcc gttcgggatc accgatccgc agttcggcct
     7921 cgacctgggc ttcttcgtct tctcgctgcc gtggctgacc ttcgcggtgg gcttcgtcac
     7981 catggcggcc gtcctggccg ccatcgccgc ggccttcacc cactacgtct acggcgggct
     8041 gcagttggcc aaccgggggc gtgacacgac ccggttcgcc ctcctgcacc tatccagcct
     8101 gtttgccgtc atcgttctct tgcgcgcggc gagctactgg ctggagcgct acaccctctc
     8161 gctgtcgacg tcggcgctga tgaccggcat ccaatacacc gacgccaacg cggtcctgcc
     8221 gaccaaggcg atcctcgccg tggcctcgct catgtgcgcc gcgatgttcc tgtcggtgat
     8281 ctggacccgg tcctggcggc tgcccgtcgt cggcaccgtc ctgttgctca tcacctcggt
     8341 cgtcgtcggc gggatcttcc cggccctcat ccagagcctc aaggtcaagc cgagcgagaa
     8401 gtcgctcgag gccccctaca tcgcccgcaa catcgaggcg acccgggcgg catacgggct
     8461 cggggctatc gaggtgtcga agtacgtgcc gagcgacgag gcgagcgccg agaacctccg
     8521 cgcgaccgcg gcagccatcc cgggcatccg catcgtcgac cccaacgtcg tctcgccgac
     8581 cgtgcgccag aagcacgccg tgcgcaacta ctacgcgttc cccgatgccc ttgacgtcga
     8641 ccgctacacg atcgacggga aactgcgcga cgtcgtcgtc gccgctcgcg agcttgacct
     8701 caccggtgtg cctgccgggc aacgcaactg gctcaacgac cacaccgtct acacgcacgg
     8761 ctacggcatc gtcgcggcat acgccaacca gcaggactcc gaggggaacc cggtctacgt
     8821 cgaggcggcg atcccggcgg ggaccgccct gggggagtac gagccccgga tctacttcgg
     8881 tgagcagtcc ccggactacg ccatcgtcgg ggctcctgag ggcgcggcac cgcgggagtt
     8941 cgactacccg gcccaggtcg agtcgcagca ggtcaacaac acgtatgccg gcaccggcgg
     9001 cgtcccgctg gacaacacga tccgcaagct ggcctacggc ttgaagtacc gcgagctcaa
     9061 cttcatgctg tccgacgcgg tcaacgcctc gtcgcggatc atggactacc gcacccccaa
     9121 ggagcgggtc gggcgggtcg ctccgtggtt gaccctcgac ggcaacgcct acccggcgat
     9181 cgtcgacggg cgcgtgctgt ggatcgtcga cggctacacg acgacggcca actaccccaa
     9241 ctcggggctc acctcgatgg cggcctcgac gcgtgactcg acgacgagca cccgacgcag
     9301 cgtcacgagc ctggagcagg gccagctcaa ctacatccgc aactcggtca aggcgaccgt
     9361 cgacgc
//
LOCUS       DAORPW010000086         9061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1825_length_9061_cov_854.110593, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000086 DAORPW010000000
VERSION     DAORPW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1825_length_9061_cov_854.110593"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLB86_08130"
     CDS             <1..402
                     /locus_tag="PLB86_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65141.1"
                     /translation="QASSAQLVTPRDLRLLTGFVAGVGVIVGAILYLSWATLSVGNQG
                     GFAISMSLYAAALALLLVIKSRARAIPRGFRRTYNIGFAVTMTAYCTGILWIFGHQSP
                     WPSASVVIPMSIVTAVPCLIAAALVHRTARA"
     gene            399..704
                     /locus_tag="PLB86_08135"
     CDS             399..704
                     /locus_tag="PLB86_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRC65142.1"
                     /translation="MTDQEQHARFRLDEVIHAPVRFSIVAALAHVEEADFAAVRDAVQ
                     VSDSVLSKQVTLLERAGYVEVKKGYVGKRPRTWLKLTPVGEAAFNGHLQALRAIAGP"
     gene            complement(723..2090)
                     /locus_tag="PLB86_08140"
     CDS             complement(723..2090)
                     /locus_tag="PLB86_08140"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012244011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HRC65143.1"
                     /translation="MSDAAFEGLLAYDRDHVWHPYSSALAPSQPYFVESAAGVRLRLR
                     LPSGEVRECIDAMSSWWCAIHGYAVPELDAAARAQLGRMSHVMFGGLTHEPGIELARR
                     LIDLAPRTTADGESPLQHVFFADSGSVSVEVAMKMALQFHVAAGRPRTRFFTIRGGYH
                     GDTFQPMSVTDPVGGMHSLFSGILPAHVFAPKPPPAWGTGNEAGAGGSESEREAALAE
                     WESATRALYAAHADSIAAVIFEPILQGAGGMSFHDPRVVQVLADLAREHGALVIFDEI
                     ATGFGRTGTMWAADQCGVVPDIMCAGKALTGGYLTLAAVLCTHEVALGVSAGEAGGLM
                     HGPTFMANPLACAVALASLELLQRNDVHRQVAGLEAGLSAGLAPARDLASVADVRVRG
                     GVGVVQLREPVRVREVAAYAIERGVWVRPFRDLVYTMPPYVTSPDDLATVCRVLTDAV
                     AAVHG"
     gene            complement(2087..2365)
                     /locus_tag="PLB86_08145"
     CDS             complement(2087..2365)
                     /locus_tag="PLB86_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65144.1"
                     /translation="MGAHDGSPLASDSLAASDGLAESDSPVAVFCPRCGIPLEHGDHT
                     VCRAALALEPPRYCPTCRRRMVVQVTPSDWTARCVEHGETTRSTWAPA"
     gene            complement(2365..3378)
                     /gene="bioB"
                     /locus_tag="PLB86_08150"
     CDS             complement(2365..3378)
                     /gene="bioB"
                     /locus_tag="PLB86_08150"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRC65145.1"
                     /translation="MTAEAVDTDILHVAREQVLERGERLTYDQIVQVLQTSDDQLDEL
                     LAIAHEVRMRFMGPDVEVEGIISLKTGGCPEDCHFCSQSGQFTSPVRSVWLNVPELVR
                     AAKLTRDTGASEFCIVAAVRGPDAKLMEQLREAVAAIRDSVDINVAASIGILTAEQAA
                     EMADMGIHRYNHNLEAGRSFFPQVVTTHSWEERWDTCQLIKDSGMELCCGGLVGMGES
                     IEQRAELADQLGELEPHEVPLNFLNPRPGTPFGDLPVMPANDALRCIAAFRLALPRTI
                     LRYAGGRELTLGDLGTRSGLLGGINAVIVGNYLTTLGRDPKEDLALLDDLKMPIKALT
                     DTF"
     gene            complement(3476..5224)
                     /gene="cydC"
                     /locus_tag="PLB86_08155"
     CDS             complement(3476..5224)
                     /gene="cydC"
                     /locus_tag="PLB86_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HRC65146.1"
                     /translation="MADAPETQQTTSAVVEAAFEAQAPGIKRAAVIGGLASASGIALT
                     ATSGWLIVAASHQPVILTLLAVIVAVRTFGIARPVLRYAERVRSHDSALDLLARRRVA
                     AYQALVPLTPARLGRRSRADVLTTAVEDLEDLVFAKVRVVVPVLGLAVAAVLTVLALG
                     LFVPAGSLVLVAVLLAQVGVSALGRRMELAAQQDWLRARAEVARTATLVTGYAAQLRG
                     IGAGPQSLGWVREAHLSLSVATRRLARARATGVALGHVVTGVGTILAALVAHWAMSVG
                     LNDAVAAVVVLAPLAVGDAFVGLPDAMGAWARARGSEARLRDLLDQPPAVHDPAPLPD
                     PLPIRGISPLPGDAGLPRISGGESSGSGLSIDVTGVAARWWPEHEGAAYDVGPVTLSI
                     GAGEHLVLTGANGSGKSTLLAVLARHLDPAAGSYCLGGVDVTTVPLAEIRAHVAVVDD
                     EPHIFASSLRENIRLARPDADDAAIEAAICAAGLAGWFDSLTEGLDTMLGTGGRGVSG
                     GERARLALARAHVSNRPVVLLDEPVAHLDHATALAVLADAHRAFAGRTVVMVSHRPDG
                     LDGFDRVVDLADLADT"
     gene            complement(5217..6809)
                     /gene="cydD"
                     /locus_tag="PLB86_08160"
     CDS             complement(5217..6809)
                     /gene="cydD"
                     /locus_tag="PLB86_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HRC65147.1"
                     /translation="MRPIDPRVFQALPEARRIMAGLGALGVALGVAAVAQAFAVSAVV
                     VAVVRFGMPGAPGLLTALLALAAVFAVRATLGALNEAYAARAGIRVSAALRERLLARW
                     LAAPVDRMPPPATASTLATQGVTSVEPYVARFLPAMVGAVVLPVFVVVTIVFLDWVSA
                     LIVVLTVPLLPVFAALIGRATQDRTERRWQALTALSGHFLDVVRGLPTLVTYGRAERQ
                     AESIRTVSEAHRAATVRTLRVAFLSSAALELLATISVAIVAVTVGLRLTYGTMELGAG
                     LACILLAPEAYWPIRRVGTEYHAAADGVAALGAALDELADAPAGVSAPAIPTRFGTDG
                     VSYRYPGTERVVVDGVTLLLPGAGLVTVTGPSGVGKSTLLELLAGLREPTAGVVSAPE
                     AHLVGQRPLLVTGSLRTNLTLGLDTPPNDDALWSALDEVGLGDDLRALAGGLDAPLGD
                     DGFGLSAGQRARLALARAILSEAAMVLLDEPTAHLDDRSAGLAHNAIQRLARTRSVVV
                     VTHRPELVELADAEVRLGGDRG"
     gene            complement(6850..7926)
                     /gene="cydB"
                     /locus_tag="PLB86_08165"
     CDS             complement(6850..7926)
                     /gene="cydB"
                     /locus_tag="PLB86_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRC65148.1"
                     /translation="MSDLSTLQIVWFVLIAVLWIGYLALEGFDFGVGMLLPILGRSHQ
                     KGAAGAADTEARKRVMLNTIGPVWDGNEVWVLTAGGAMFAAFPQWYATLFSGFYLALA
                     LILVALIVRGMGLDYRHKVDSDTWRKRWDLAITTGSFLAALLWGVGLTNIVYGTPIRP
                     NPDVKGDYFFDGNLFTLLGVVPLIGGLTFVGLFLTHGAFFIALKTDGPIRQDARALGI
                     KTGLVTAVLAVVLLLAINVRHGSLVSWIGTVVAAVALLGGIFMATKGREGWAFLGTFV
                     CIAFAVVAYFGALFPNAMPTTLADGADLTLAAASSTPKTLTIMTWAAAVFTPIVLLYQ
                     GWTYWVFRKRISTKNIPAPVTVGK"
     gene            complement(7943..>9061)
                     /locus_tag="PLB86_08170"
     CDS             complement(7943..>9061)
                     /locus_tag="PLB86_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRC65149.1"
                     /translation="ESTFLGLWIFGWDRLPKKIHLASIWLVHIGTVFSAFFILAANSF
                     MQNPVGYTYNAQTNRAELDDFAAVLFNKVQLVTFPHVVLGAYMTAGAFVMGIALYHLR
                     KQALDKGPDISMYRKAVRFGAIVALVASIGVLVSGDVQGKVMTEVQPMKMAAAEGLYE
                     TRSGAPFSILTIGSLDGTSARPILEVPGLLSFLGTGSFSGEIQGINDLQAADKAEFGN
                     NPQTASASYVPVIWMTYWNFRLMMGAGFLTMLFAAMALWKTRGQGVVTSGMWKHIAIW
                     TPLLPVLANSFGWIFTEVGRQPWLVNGLMTTETGVSTSVSATSVWISMIIYTLLYGAL
                     AVVELRLLLRYIQPGAVPVEAPKDPADRADDEPFVFAY"
BASE COUNT         1525 a         3075 c         3215 g         1246 t
ORIGIN      
        1 caggcgtcga gtgcccaact cgtgacccct cgtgacctgc gtctcctcac cggcttcgtc
       61 gcaggtgtcg gcgtgatcgt gggggcgatc ctctatctca gctgggccac tttgtcagtg
      121 ggcaaccagg gcggcttcgc catcagcatg tccctgtatg ccgccgccct cgccctcctg
      181 ctcgtcatca agtcccgggc aagggccatc cctcgagggt tccggcggac ctacaacatc
      241 ggcttcgccg tgacgatgac cgcctactgc accgggattc tgtggatctt cgggcatcag
      301 tcgccgtggc cgagcgcgtc cgtcgtcatc ccgatgagca tcgtgacggc agtgccgtgc
      361 ctcatcgccg cggcgctggt tcaccggacg gcacgggcat gaccgaccag gagcagcacg
      421 cgcggttccg gctcgacgag gtgatccacg cgccggttcg attctcgatc gtcgcggcgc
      481 tcgcccacgt cgaggaggcc gatttcgcgg cagtgcgcga tgcggtccag gtgagcgact
      541 cggtcctgtc caagcaggtg accctgctcg agcgcgctgg ctacgtcgag gtgaagaagg
      601 ggtacgtcgg caaacggccc cgcacctggc tcaaactgac gcctgtggga gaggccgcgt
      661 tcaacgggca cctccaggcc ctgcgcgcga tcgccggacc gtgaccgcag cgccgacgac
      721 cgtcagccgt ggacagcggc gacggcgtcg gtgagcacgc ggcataccgt cgcgaggtcg
      781 tcgggggagg tgacgtaggg gggcatcgtg tagacgagat cgcggaacgg ccgcacccac
      841 accccccgct cgatcgcgta tgccgcgacc tcccgcacgc gcactggctc gcgcagttgg
      901 acgacgccaa cacccccgcg cacccggacg tcggcgaccg acgccaggtc gcgagccggc
      961 gccagtcccg ccgacagccc cgcctcaagc cccgccacct gacgatgaac atcgttgcgt
     1021 tgcaacaact ccaggctcgc cagggccacc gcgcaggcca gcggattggc catgaacgtc
     1081 ggcccgtgca tcagcccacc ggcctcgccg gcactgaccc ccaacgcaac ctcgtgggtg
     1141 cacaacacgg cggcgagcgt gaggtagccc ccggtcagcg ccttccccgc acacatgatg
     1201 tcgggaacca cgccgcactg gtcggccgcc cacatcgtcc ccgtccggcc gaacccggtc
     1261 gcgatctcgt cgaagatcac cagcgccccg tgctcgcggg ccaggtcggc cagcacctgc
     1321 acgacccggg ggtcgtggaa gctcatccca ccggcgcctt gcaggatcgg ctcgaagatg
     1381 accgcggcga tggagtcggc gtgcgcggca tacagggctc gcgtggcgct ctcccactcg
     1441 gcgagggccg cctcgcgctc gctctcgctg ccgcccgcgc cggcttcgtt ccccgtgccc
     1501 cacgcgggcg gcggtttggg ggcgaagacg tgcgccggga gtatgccgct gaacaggctg
     1561 tgcatcccgc ccaccgggtc ggtgaccgac atgggttgga aggtgtcgcc gtgatagccg
     1621 ccgcggatcg tgaagaagcg agtgcgtggc cgtccggccg caacatggaa ctgcagagcc
     1681 atcttcatgg cgacctcgac cgagacagag cccgagtcgg cgaaaaacac atgctggagc
     1741 ggggattcac catcggctgt ggtcctcgga gccaggtcga tgagtcgccg ggccaactcg
     1801 atccccggct cgtgggtgag cccgccgaac atcacgtggg acatgcgccc cagctgagcc
     1861 cgcgcggcgg cgtcgagctc gggcacggca tacccatgga tcgcgcacca ccacgaggac
     1921 atggcgtcga tgcactcgcg gacctcgccg ctagggagtc gcagccgcag ccgcacgccc
     1981 gccgccgact cgacgaagta gggctgggac ggggcgagcg cggaggagta ggggtgccag
     2041 acgtggtcgc ggtcgtaggc gagcagcccc tcgaatgcgg cgtcgctcac gccggcgccc
     2101 acgtgctgcg ggtcgtctcc ccgtgctcga cgcagcgcgc cgtccagtcc gacggggtca
     2161 cctggacgac catccggcgc cggcacgtgg ggcagtagcg cggcggctca agggcgagcg
     2221 ccgcgcggca taccgtgtgg tcaccgtgct ccagaggtat gccgcatcgc gggcagaaca
     2281 ccgcgaccgg gctgtccgac tcggcgaggc cgtcagatgc ggccaggctg tcggaagcga
     2341 gcggagagcc gtcgtgcgca cccatcagaa cgtgtccgtg agggccttga tcggcatctt
     2401 gaggtcatcg agcagcgcga ggtcttcctt ggggtcgcgg cccagcgttg tgaggtagtt
     2461 gccgacgatg accgcgttga tgccgccgag caggccgctg cgggtgccga ggtcgccgag
     2521 ggtcaactcg cgcccaccgg cataccgaag gatcgtgcgg ggcagcgcga ggcggaaggc
     2581 ggcgatacag cgcaaggcgt cgttggccgg catgacgggg aggtctccga acggcgtccc
     2641 ggggcgcggg ttgaggaagt tgagcggcac ctcgtgcggc tccaactcgc ccaactggtc
     2701 ggcgagttcg gcgcgctgct cgatgctctc gcccatgccg acgagcccgc cgcagcacag
     2761 ctccatgccg ctgtctttga tgagttggca ggtgtcccaa cgctcttccc acgagtgggt
     2821 ggtgaccacc tgggggaaga acgagcggcc ggcctcgagg ttgtggttgt aacggtggat
     2881 gcccatgtcg gccatctcgg ccgcctgctc ggcggtgagg atgccgatcg acgcggcgac
     2941 gttgatgtcg acgctgtcgc ggatcgcggc gacggcctcg cgcagctgct ccatgagctt
     3001 ggcgtcaggc ccgcgcacgg cagcgacgat gcagaactcg ctggcgcccg tgtcgcgggt
     3061 cagcttggcc gcgcggacca actcgggcac gttgagccac accgaacgca ccggcgaggt
     3121 gaactgcccc gactgcgagc agaagtggca gtcctcgggg caaccgcccg ttttgaggct
     3181 gatgatgccc tcgacttcga cgtcgggccc catgaaccgc atccgcacct cgtgcgcgat
     3241 ggcgagcaac tcgtcgagct ggtcgtcact ggtctgcagg acctgcacga tctggtcata
     3301 ggtcagccgc tcgccgcgct cgaggacctg ctcgcgcgcc acgtggagga tgtcggtgtc
     3361 cactgcctct gccgtcatga ccgcgattct tccgtatgcc gcgcccgctc gctcccagcc
     3421 gttggtcccg aaccgttgtc gggggtcagt tgacctcgat ttcgggcccg gccagtcagg
     3481 tgtcggcgag gtcggccagg tcgacgaccc ggtcaaagcc gtcgaggccg tccgggcggt
     3541 ggctgaccat gacgacggtg cgcccagcga aggctcggtg cgcgtcggcc aacacggcca
     3601 gcgctgtggc gtggtccagg tgcgcgaccg gttcgtcgag caggacgacc ggacggttgc
     3661 tgacgtgggc ccgcgcaaga gccagcctgg cccgctcacc gccagacacg ccgcgcccac
     3721 cggtgccgag catggtgtcc agcccctcgg tgaggctgtc gaaccatccg gcaagcccgg
     3781 cggcgcagat cgcagcctcg atcgcggcgt cgtctgcgtc gggccgggcg aggcggatgt
     3841 tctcgcgcag ggacgacgcg aagatgtgtg gttcgtcgtc gacgacggcc acgtgggcgc
     3901 ggatctcggc cagcggcacc gtggtgacgt cgaccccgcc gaggcaatag ctgcccgccg
     3961 ccgggtccag atggcgcgcc agcacggcga gcagggtcga cttgccgctc ccgttggcgc
     4021 cggtgaggac aagatgctcc ccggcaccga tgctcaaagt caccggcccg acgtcgtatg
     4081 ccgccccctc gtgctccggc caccagcggg cggccactcc cgtcacgtcg atgctcagcc
     4141 ccgaaccgct gctctcccct ccgctgattc gaggtaatcc ggcatcgcct ggcaatggcg
     4201 aaatacctcg aatgggcagg gggtccggca ggggggcggg gtcgtgcacc gccgggggct
     4261 ggtcgagcag atcacgcagt cgggcctcgc ttcctcgcgc cctggcccac gcacccatcg
     4321 cgtccggcag ccccacgaac gcatcaccca ccgctagcgg ggccagcacc acgacggccg
     4381 ccaccgcatc gttgagcccg acactcatcg cccaatgggc cacgagggcc gcgaggatcg
     4441 tcccgacacc cgtcacgaca tgccccaacg ccaccccggt cgcccgggct cgagccagcc
     4501 gtcgcgtcgc aaccgacaaa gacaggtgtg cttcgcggac ccagccgagc gactgcgggc
     4561 cggcgccgat gccccggagc tgcgcggcat acccggtgac gagagtcgcg gtgcgcgcca
     4621 cctcggcgcg agcgcgcagc caatcctgtt gtgccgcaag ctccatacga cgcccaagcg
     4681 cgctgacacc gacctgcgcg agcaggacgg cgacaagcac cagcgatccg gccggcacga
     4741 agagtcccag agccaagacg gtgagcacag ccgcgacggc cagcccgagc accggcacga
     4801 cgacccgtac cttggcgaac accaggtctt cgaggtcctc gaccgccgtc gtgaggacgt
     4861 cagcgcgcga gcgtcgcccc agccgggccg gggtgagcgg cacgagtgct tggtatgccg
     4921 ccacccgccg ccgcgcgagg aggtccaggg cgctgtcgtg tgagcgcacc cgctcggcgt
     4981 aacgcagcac cgggcgggcg atgccgaagg tccggaccgc gacgatgacg gccaagaggg
     5041 tgaggatcac gggttgatga ctcgcggcga cgatgagcca gccagaggtc gccgtgaggg
     5101 cgatgcccga cgccgaggcg agcccaccga tgaccgctgc gcgcttgatg cctggggcct
     5161 gcgcctcgaa cgcggcctcg acgaccgcag aggtcgtttg ctgggtttcg ggagcgtcag
     5221 ccacggtcgc ctcccaaccg aacctcggcg tcagcgagct cgaccagctc gggacgatgg
     5281 gtgacgacga cgaccgatcg ggtccgcgcc agacgctgaa tcgcgttgtg ggccagccct
     5341 gctgagcgat cgtccaggtg cgcggtcggc tcatcaagca gaaccatcgc ggcctccgac
     5401 aagatcgccc gagccaacgc cagccgggcg cgctgaccgg cggacagtcc gaaaccgtcg
     5461 tcgcccagcg gtgcgtcgag cccacccgcc agcgctcgca agtcgtcgcc gagcccgacc
     5521 tcgtcgagcg ccgaccagag ggcgtcgtcg ttgggcggtg tgtccagccc gagggtgagg
     5581 ttggtccgca gcgagccggt gaccagcagc ggccgctgtc cgacgaggtg cgcttcgggg
     5641 gcggacacga ctccggcggt cggctcccgc aggcccgcga gcagttcaag cagcgtcgat
     5701 ttgccgaccc cggacggtcc ggtgacggtc accaagccag cacccgggag gagcaaggtc
     5761 acgccgtcca caacgacccg ttcggtgccg gggtagcgat aactcacccc atcggtcccg
     5821 aaccgagtgg ggatcgcggg agccgacaca cctgctggag catcggccaa ctcgtcgagg
     5881 gcagcgccca gggcggcgac cccgtcagca gctgcgtgat actcggtccc gacccggcgg
     5941 atcggccaat aagcctctgg ggcaaggaga atgcacgcca agccagcccc gagctccatc
     6001 gtcccgtagg tcagccgaag cccaaccgtc accgcgacga tcgccaccga gatcgtcgcc
     6061 aacaactcca gcgcagccga ggacaagaag gccacgcgca acgtccgcac cgtggccgcc
     6121 cgatgcgcct cgctcaccgt ccggatcgac tcggcctgcc gctcggcccg cccataggtc
     6181 accaacgtcg gcagcccccg gacgacgtcg aggaagtgcc cggacagtgc cgtgagcgcc
     6241 tgccagcgac gctcggtgcg gtcctgggta gcccgaccga tcagtgcggc gaagaccggc
     6301 agcaacggca ccgtcaggac gacgatgagg gcgctcaccc agtcgaggaa cacgatcgtc
     6361 accacgacga agaccggcag gaccaccgcc ccgaccatgg ccgggaggaa tcgggcgaca
     6421 tagggctcga cgctcgtgac cccctgtgtt gcaagggtgc tcgcggtggc gggcggcggc
     6481 atacggtcga cgggagcagc gagccaccgg gccaggaggc gctcacgcaa ggcagcagac
     6541 actcgtatgc cggcccgcgc ggcatacgcc tcgttgaggg ctcccagcgt ggcgcggacc
     6601 gcgaagacgg ccgcgagcgc gaggagggcg gtgagcaggc cgggggcacc cggcataccg
     6661 aagcgaacga cggcgaccac gacggccgag acggcgaagg cttgggcgac ggcggcgacg
     6721 ccgagtgcca cgcccaacgc cccgaggccc gccatgatgc ggcgggcctc ggggagggcc
     6781 tggaagaccc gggggtcgat cggtcgcacg ggttgatgct aggtgagccg cgaggcggtc
     6841 acccgctcgt cacttgccga cggtgaccgg ggcggggatg ttcttcgtgg agatccgctt
     6901 gcggaacacc cagtaggtcc agccctggta gagcaggacg attggagtga agaccgcggc
     6961 ggcccaggtc atgatcgtca gcgtcttcgg tgtgctcgac gcggcggcga gggtgaggtc
     7021 ggcgccgtcg gcgagcgtcg tgggcatcgc gttcgggaac agcgccccga agtaggccac
     7081 gacagcgaag gcgatgcaga cgaaggtgcc gaggaacgcc cagccctcac gacccttggt
     7141 ggccatgaag atgccgccga gcagggcgac tgccgccacg accgtgccga tccagctgac
     7201 cagcgacccg tgccgcacgt tgatggccag caacaggacg acggcgagga ccgcggtgac
     7261 gaggccggtc ttgatgccca acgcgcgggc gtcctgacgg atcgggccgt cggtcttgag
     7321 cgcgatgaag aaggcgccgt gggtgaggaa cagcccgacg aaggtgagcc caccgatgag
     7381 gggcacgacg cccagcaggg tgaacaggtt gccgtcgaag aagtagtcac ccttgacgtc
     7441 cgggttgggc cggatcgggg tgccgtagac gatgttcgtc aggccgacgc cccacagcaa
     7501 cgcggcgagg aacgacccgg tggtgatggc gaggtcccag cgcttgcgcc acgtgtcgga
     7561 gtcgaccttg tggcggtagt ccaggcccat gccgcggaca atgagggcga cgaggatgag
     7621 ggccagggcg aggtagaagc cgctgaagag ggtggcgtac cactgcggga aggccgcgaa
     7681 catcgcgccg cctgcggtga ggacccagac ctcgttgccg tcccagaccg ggccgatggt
     7741 gttgagcatg acgcgcttgc gggcctcggt gtcggccgcg ccggcggcgc ccttctggtg
     7801 gctgcggccg aggatgggca ggagcatgcc gaccccgaag tcgaagcctt cgagggcgag
     7861 gtagccgatc cagaggacgg cgatgaggac gaaccagaca atctggaggg ttgacaggtc
     7921 agacatggtc agggtccttg gctcagtagg cgaagacgaa gggttcgtcg tcggcccgat
     7981 cggcggggtc cttgggtgcc tcgacgggca ccgcccccgg ctggatgtag cgcagcagga
     8041 gccgcaactc gacgacggca agcgcgccgt agagcagggt gtagatgatc atcgagatcc
     8101 acacgctggt ggccgagacc gaggtggaga cgccggtctc ggtcgtcatc aggccgttga
     8161 cgagccaggg ctgacggccg acctcggtga agatccaccc gaaggagttg gccagcaccg
     8221 gcagcagcgg cgtccagatg gcgatgtgct tccacatgcc gctggtgacg acgccttggc
     8281 cgcgggtctt ccacagcgcc atggcggcga acaacatcgt gaggaacccg gcgcccatca
     8341 tcaggcggaa gttccagtag gtcatccaga tcaccgggac gtaggacgcc gaggcggtct
     8401 gcgggttgtt gccgaactcg gccttgtcag ccgcctggag atcgttgatg ccttggatct
     8461 cgccggagaa cgagccggtg ccgaggaagg acagcagacc ggggacctcg aggatcgggc
     8521 gggccgacgt gccgtcgagc gagccgatcg tgaggatcga gaacggcgcg ccggagcggg
     8581 tctcgtacag tccctctgcc gcagccatct tcatcggctg gacctcggtc atcaccttgc
     8641 cctggacgtc gccggagacc aggacgccga tgctggcgac gagggcgacg atcgcgccga
     8701 agcgaacggc cttgcggtac atcgagatgt ccgggccctt gtcgagcgcc tgcttgcgca
     8761 ggtggtagag cgcgatgccc atgacgaacg cgccggcggt catgtaggcc cccagcacga
     8821 cgtgcgggaa cgtcaccaac tgcaccttgt tgaacagcac ggcggcgaag tcgtcgagct
     8881 cggcgcggtt ggtctgagcg ttgtaggtgt agccgaccgg gttctgcatg aacgagttgg
     8941 cggcgaggat gaagaacgcc gagaacaccg tgccgatgtg cacgagccag atggaggcca
     9001 ggtggatctt cttgggcaac cggtcccaac cgaagatcca caggccgagg aaggtcgact
     9061 c
//
LOCUS       DAORPW010000087         9046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1828_length_9046_cov_686.983428, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000087 DAORPW010000000
VERSION     DAORPW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1828_length_9046_cov_686.983428"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1502)
                     /locus_tag="PLB86_08175"
     CDS             complement(3..1502)
                     /locus_tag="PLB86_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRC65150.1"
                     /translation="MSVPTHTTPPPPIPQEVRDWVPATRTLLVGGSMVEPRGETWDVF
                     NPATEAVIATVSGASADQVDAAVLAARNAFPAWAALSGAERGAYIHRFADALEAAADR
                     LLPSIVNEVGTPVSLAEYLQVKMAVQEHLRWAADAAATDRTVHLGRYETPWPTMSDVV
                     HEPVGVVAAITGYNYPLNLAIFKFGAALAAGCTVVLLPSPRTPLTTLLLGDLIQESGL
                     PPGVMNVIIGGADVGRQLSSHPDVDRVSFTGSDGVGALIMEQAARNLAGVTLELGGKS
                     PSIVMPDVDVAKVAVEMHLRWSRNAGQGCAALARLLVHESRFDEFVEAGASAFDQMVV
                     GDPWDRATNIGPLIRPDHQARVQSFITDSLAVGGRKLLEVTKPLPEKGWYVNPVLLGG
                     LDHSARACQEEIFGPIAVLVPFKDTEDAIRLANDTKYGLAANVWCNDPIEARRVADRV
                     RAGTVWINGGGGMRPDAPFGGFGRSGIGRELGEWGMREYLEVKHVQWRL"
     gene            complement(1499..3241)
                     /locus_tag="PLB86_08180"
     CDS             complement(1499..3241)
                     /locus_tag="PLB86_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC65151.1"
                     /translation="MSQPISQPMTYRDLVPTFPDPTTWTLDQVLRHHAQVRPDALALE
                     CPEESVTLTYAAGLADAERLASGMLAAGARPGDRVVIMAANSSRFVRSWWATAVSSLV
                     EVPVNTNYEGEFLRHQVTVVQPRFAVIDDTYAERFVAIKEQVRVIEEFWVIDTGAGIH
                     DDAIDLLREHGWVAGDWEELLQAEPVALTAPSPQDLGAIFFTSGTTGPSKGVAMPNSQ
                     LYFFAQEVACFTRLTPEDVYLTTTPLFHGNAQFMAAYPALLAGGRAVVRPKFSASRWI
                     DHVRDSGVTVTNFVGVMMDFAWKQAPRPDDRNNHLRCVYAAPTASSIVQEFKERYGIE
                     AFVDAFGLTETSAPIISPYGVPRPAGAAGLQASDWFDIRLVDPETDREVAVGEIGELV
                     VRPVHPWTCSMGYYGMPEKTVEAWRNLWFHTGDALRKDADGWFYFVDRYKDALRRRGE
                     NISSYEVEQAILGHPAVTECAVIGVPADVEAGEDEVLAAVVVDGPVDPAEILAWCAGR
                     IPAFAVPRFMRVVDALPKTPSEKVRKAALRADGIPPGTYDRERSATVPAASPPATPAP
                     ATPAPATQTEEAHA"
     gene            complement(3238..4293)
                     /locus_tag="PLB86_08185"
     CDS             complement(3238..4293)
                     /locus_tag="PLB86_08185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRC65152.1"
                     /translation="MVNVGLLISDIPRSTPSKQHFSDLLRFVEAAQRNGFTYLAMGQH
                     FLYGDLRWLQPVPLFARLAAEVDPDFKLVTQIIIAPLYHPVLLAEEIATLDIVTEGRL
                     IFGAGGGYRPEEFTFLDVPYKERGARFDESLELMTKLWTQETVTHHGRFWNLDGVQPH
                     IQPVQQPHPPIWIGAHAEAGVRRAAKYGDAYACPPETPVREVARRYAIVRDGFAARGK
                     AFTSQPLRRNIQVGDTREEAMVEYARVAQGRYLTYLNKGLDVMANTDLENEFAKAVSS
                     HAVIGTDDQVAAELCDLVQTLPVDPILLRPTWPTMSSDEMIAALDRLGRTVVPALRAI
                     TPRTDIDEALLAPQEDQ"
     gene            complement(4327..>5088)
                     /locus_tag="PLB86_08190"
     CDS             complement(4327..>5088)
                     /locus_tag="PLB86_08190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC65153.1"
                     /translation="ADENPGRGVVVTGAARGMGKAIAQRFAASGHVVIGVDRDADALA
                     ATFAELGAPHVGIAGDVCDEELLARVCEQAVEVGGGLRCFVANAGIIGPGPSTEQPVA
                     QWDGLLGVNLTGVFLGARHAARVMRPGASIVATSSICGSLGFGGRAAYGASKAGINGL
                     VWALAVEWGPLGIRVNAVAPGAIRTELAAAMIATGRVDESVYEARIPMGRRGRPEEVA
                     AAVHFLASDDASYVSGVVLPVDGAWAINGLPAQAG"
     assembly_gap    5089..5188
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5218..5982)
                     /locus_tag="PLB86_08195"
     CDS             complement(5218..5982)
                     /locus_tag="PLB86_08195"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC65154.1"
                     /translation="MAVVVGGGSGMGRAISERLASEGAIVYVADAREQAAYEVASAIT
                     ASGSAAIPYAAIPHVVDATSVDALRALFERVEADHGVLHVFHHQVGAPGAGGIDFSEN
                     EFDDAVAVNIKSATFGASLAWPLLSRAEGKASVTFTASTAALVGSPFSPLYSLTKGAL
                     VAFARSLALVGAPVGIRVNVVAPGPVDTPMLPTFFGREPGASIADLMDSFLATVPLGR
                     PGTPGEVAGTVAFLASDDAGFITGITIPVDGGLTAR"
     gene            complement(6069..6587)
                     /locus_tag="PLB86_08200"
     CDS             complement(6069..6587)
                     /locus_tag="PLB86_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65155.1"
                     /translation="MGRVGIDDLEALQGLGLRHEERAELLETQSECTFVFTSEAGWPS
                     GVVMTYIHIDGVFWLTAVEGRGQTKGIAKDPRATIVVSNRGTDLPGRQMAAYRCVVTV
                     HRDRRTKAWFIDEFTKRLQPGNEAPWMKLLDSPNRVIFKAQPVALAVSHDSRKIPGGG
                     RNTTGSSAGGGE"
     gene            6755..>7203
                     /locus_tag="PLB86_08205"
     CDS             6755..>7203
                     /locus_tag="PLB86_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRC65156.1"
                     /translation="MSNAVIVEAVRTPMGRGKAGGALADLHPVDLLAQTLTALVARTG
                     IDPGIVDDVIIGCVGQNLEQSATPGRQAWLAAGFPEHVPSVTIERKCGSGQQAIEFAA
                     AGIVAGLYDIVIAGGVESMSRVPIMSARMGADPHGPGVRARYPGLVP"
     assembly_gap    7204..7303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7304..7645
                     /locus_tag="PLB86_08210"
     CDS             <7304..7645
                     /locus_tag="PLB86_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65157.1"
                     /translation="VGDDPQLMLTGPIAATTKVLARAGLTLRDIDTVEVNEAFASVPL
                     AWLAEHPYDEERLNPRGGAIALGHPLGASGARIMTSMLHHLEQTGGRYGLQTICEAGG
                     MANATILERLG"
     gene            7735..8499
                     /locus_tag="PLB86_08215"
     CDS             7735..8499
                     /locus_tag="PLB86_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC65158.1"
                     /translation="MHLENSSAVVTGGASGLGLATVQALLARGVLVTILDLPTSQGAQ
                     VAESLGAAASFAPGDVRDDEAVAAAVALADGRAPLRTLVHCAGRGGTVRVLDKAGAPG
                     DYDLYREIVAVNLFGTFNVLRHAASAMATHEPIDGERGVCVLTASVAAFEGQIGQIPY
                     ASAKAGVVGMTIVAARDLAQRQIRVCTIAPGVFDTPILSRFSQDIKDGLAAQVPHPSR
                     LGRPEEYAATAMHIVDNAMLNGETIRLDGAIRMSPR"
     gene            8496..>9046
                     /locus_tag="PLB86_08220"
     CDS             8496..>9046
                     /locus_tag="PLB86_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC65159.1"
                     /translation="MTEGLGGSLGEGGAMGSPVHTEVRGGVLLIVLNRPEIKNAINTE
                     TAEAVAAALDRLDSDPALTCGVLTGAGGTFCAGMDLKAFLAGERPSVPVRGFAGIVER
                     PSDKPLVAAVEGAAVAGGFEIALACDLVVAGESARFGLPEVRRGLVAAGGGLLRLAER
                     IPTAIAMEWALTGALVGAQRAFEV"
BASE COUNT         1292 a         3061 c         3183 g         1310 t
ORIGIN      
        1 catcagagcc gccactggac gtgcttgacc tcgaggtatt cgcgcatgcc ccactcaccg
       61 agctcgcgcc cgatgccgga gcggccgaac ccgccgaagg gggcgtccgg gcgcatcccg
      121 ccgccgccgt tgatccacac cgtgccggcc cgcacccggt cggcgacccg gcgggcctcg
      181 atcgggtcgt tgcaccagac gttggcagcc aggccgtact tcgtgtcgtt ggccagccgg
      241 atcgcgtcct cggtgtcctt gaacggcacc aacaccgcga tcgggccgaa gatctcctcc
      301 tggcaggccc gcgcactgtg gtcgagcccg ccgaggagca cggggttgac gtaccaaccc
      361 ttctccggca gcggcttggt cacctcgagc agtttgcgcc caccaacagc gagcgagtcg
      421 gtgatgaagg actgcacccg agcctggtga tccggacgga tgagcgggcc gatgttggtc
      481 gcgcggtccc acgggtcgcc gacgaccatc tggtcgaagg ccgacgcccc cgcctcgacg
      541 aactcgtcga accggctctc gtggacgagc aagcgcgcca gcgccgcaca accctggccg
      601 gcgttgcgcg accagcgcag gtgcatctcg accgcgacct tggcgacgtc gacatcgggc
      661 atgacgatgc tcggcgactt gccgccgagc tccagcgtga ccccggccag gttgcgcgcg
      721 gcctgctcca tgatgagcgc gccgaccccg tcggacccgg tgaacgacac ccggtcgacg
      781 tcggggtgcg aggacaactg ccgccccacg tcggcgccgc cgatgatgac gttcatgacg
      841 cccgggggca gccccgactc ttggatgagg tcgccgagca acagggtcgt cagcggcgtg
      901 cgcggcgacg gcagcaacac gaccgtgcac cccgcggcca gcgccgcacc gaacttgaag
      961 atcgccaggt tgagtgggta gttgtagccc gtgatcgccg cgacgacccc gaccggctca
     1021 tgcacgacgt cgctcatcgt cggccacggc gtctcataac gccccaggtg gacggtgcga
     1081 tcggtcgccg ccgcgtcggc cgcccagcgc agatgctcct gcacggccat cttgacctgc
     1141 aggtattccg cgagcgagac cggcgtcccg acctcgttga cgatcgaggg cagcagtcgg
     1201 tcggcggcgg cttcgagcgc gtcggcgaag cggtggatgt aggcgccgcg ctcggcaccc
     1261 gacagcgccg cccacgccgg gaaggcgttc cgcgcagcca gcacggcggc gtcgacctga
     1321 tcggcgctcg ccccgctgac ggtcgcgatg acggcctcgg tcgcggggtt gaagacgtcc
     1381 cacgtctcgc cgcgcggctc gaccatcgag ccgccgacga gcagtgtgcg ggtcgccggg
     1441 acccagtcgc gcacttcctg cgggatcggc ggcggcggag tggtgtgggt gggcacgctc
     1501 atgcgtgggc ctcctcggtc tgggtcgcgg gagcaggggt cgcgggagca ggggtggcag
     1561 ggggagacgc agcaggaacg gtcgccgacc gctcccggtc atacgtgcca gggggtatgc
     1621 cgtccgcccg cagcgcggcc ttgcgcacct tctcggacgg ggtcttcggc agggcgtcga
     1681 cgacccgcat gaaccgcggg acggcgaagg cggggatccg gccggcgcac cacgcgagga
     1741 tctcggcggg gtcgacgggg ccatcaacga cgaccgccgc gagcacctcg tcctcgccgg
     1801 cctcgacgtc ggcgggcaca ccgatgacgg cgcactcggt gaccgccggg tggccgagga
     1861 tggcctgctc gacctcgtag gagctgatgt tctccccgcg ccgacgcagc gcgtccttat
     1921 agcggtcgac gaagtagaac cacccgtcgg cgtccttgcg cagcgcgtca ccggtgtgga
     1981 accacaggtt gcgccaggcc tcgacggtct tctcgggcat cccgtaatag cccatgctgc
     2041 aggtccacgg atgcaccggc cgcacgacca actcgccgat ctcgccgact gccacctcgc
     2101 gatcggtctc agggtcgacc agccggatgt cgaaccagtc cgacgcctgc agccccgccg
     2161 cacccgcagg ccgcggcacg ccgtagggcg agatgatcgg cgcgctcgtc tcggtcagcc
     2221 cgaacgcgtc gacgaacgcc tcgatgccat accgctcctt gaactcctgg acgatcgagg
     2281 aggcggtggg ggcggcatac acacagcgca ggtggttgtt gcggtcgtcc gggcgcggcg
     2341 cctgcttcca cgcgaagtcc atcatcacgc cgacgaagtt ggtgaccgtc acgcccgagt
     2401 cgcgcacgtg gtcgatccac cggctcgccg agaacttcgg ccgcaccacc gcgcgccctc
     2461 ccgcgagcaa ggcggggtat gccgccatga actgcgcgtt cccgtggaag agcggtgtcg
     2521 tcgtgaggta gacgtcctcg ggcgtgaggc gggtgaagca ggccacctcc tgggcgaaga
     2581 agtagagctg cgagttgggc atcgcgacgc ccttgctcgg cccggtcgtg cctgaggtga
     2641 agaagatcgc gccgaggtcc tgcggcgagg gcgcggtcag cgcgacgggc tcggcctgca
     2701 ggagctcctc ccaatcgccg gccacccagc cgtgctcgcg cagcaggtcg atggcgtcgt
     2761 catggatccc ggccccggtg tcgatgaccc agaactcctc gatgacccgc acctgctcct
     2821 tgatggcgac gaagcgctcg gcatacgtgt catcgatgac ggcgaaccgt ggctggacga
     2881 cggtcacctg gtggcgcagg aactcgccct catagttggt gttgaccggc acctcgacga
     2941 ggctggacac ggccgtcgcc caccaactgc gcacgaaccg cgaggagttg gcggccatga
     3001 tgacgacccg atcgcccggc cgggctccgg ccgcgagcat ccccgaagcg agccgttccg
     3061 cgtcggccag ccccgcggca tacgtgaggg tgacggactc ttccgggcac tccagggcga
     3121 gcgcgtcggg ccgcacctgc gcgtggtgac gcagcacctg gtcgagcgtc caggtcgtgg
     3181 ggtcggggaa ggtcgggacg aggtcccgat aggtcatcgg ctgactgatc ggctggctca
     3241 ctggtcctcc tgcggggcga gcagcgcctc gtcgatgtcg gtgcgggggg tgatcgcgcg
     3301 cagcgcgggg acgacggtgc gaccgagccg gtccagggcc gcgatcatct cgtcgctgga
     3361 catcgtcggc caggtcgggc gcagcaggat cgggtcgacg ggcaacgtct ggacgaggtc
     3421 gcacaactcg gcggccacct gatcgtcggt cccgatgacg gcgtgcgacg acacggcctt
     3481 ggcgaactcg ttctccaggt cggtgttggc catgacgtcc aggcccttgt tgagataggt
     3541 gagataacgc ccctgggcga cccgggcgta ttccaccatc gcctcctcgc gggtgtcgcc
     3601 gacctggatg ttgcgccgca gcggctgcga ggtgaaggcc ttgccgcgcg cggcgaatcc
     3661 gtcgcggacg atcgcatagc ggcgagcgac ctcgcgcacc ggggtctcgg gcgggcaggc
     3721 ataggcgtcg ccatactttg ccgcgcggcg gacgccggcc tcggcatggg ccccgatcca
     3781 gatcggcggg tgcggctgct ggacgggctg gatgtgcggc tggacgccgt cgaggttcca
     3841 gaaccggccg tggtgggtga ccgtctcctg ggtccagagc ttggtcatca gctcgagcga
     3901 ctcgtcgaag cgggcgccgc gctccttgta aggaacgtcg aggaaggtga actcctcggg
     3961 ccgatatccg ccacccgccc cgaagatcag ccgaccttcg gtgacgatgt cgagggttgc
     4021 gatctcctcg gcaagcagca ccgggtgata gagcggcgcg atgatgatct gggtgacgag
     4081 cttgaagtcg gggtcgacct cagccgccag ccgcgcgaac aacggcaccg gctgcagcca
     4141 gcgaaggtcg ccgtagagga agtgctgccc catcgccaga taggtgaacc cgttgcgttg
     4201 ggccgcctcg acgaagcgca gcaggtcgct gaagtgctgc ttggacggcg tcgaccgggg
     4261 gatgtcgctg atgagcagcc cgacgttgac catgaggttc ctcttccttc tgcttcgttc
     4321 cgcggctcag ccggcctgcg ccggaaggcc gttgatcgcc cacgcgccgt cgaccggcag
     4381 gacgaccccc gacacatagc ttgcgtcgtc cgaggcgagg aagtgcacgg cggccgcgac
     4441 ctcctcggga cgaccgcgcc gccccatggg gatgcgcgcc tcgtaaacac tctcgtcgac
     4501 ccgaccggtc gcgatcatcg cggcggcgag ctcggtgcgg atcgcgccgg gggccaccgc
     4561 gttgacgcgg atcccgagcg gcccccactc caccgccagc gcccacacca gtccgttgat
     4621 ccccgccttg gacgcaccgt atgccgcgcg cccaccgaag cccagcgacc cacagatcga
     4681 gctggtcgcc acgatgctgg cgccgggtcg catgacccga gcggcatgcc gagcgccgag
     4741 gaagacgccc gtgaggttga cgccgagcag gccgtcccat tgggccaccg gttgctcggt
     4801 gctcggtccg gggccgatga tgccggcgtt ggcgacgaag cagcgcagcc cgccgccgac
     4861 ctcgaccgcc tgctcgcaga cccgcgcgag caactcctcg tcgcagacgt cgccggcgat
     4921 gccgacgtgg ggggcgccga gctcggcgaa ggtcgcggca agcgcgtcgg cgtcgcggtc
     4981 gaccccaatg acgacgtggc cggacgcggc aaagcgctgt gcgatcgcct tacccatgcc
     5041 cctggcggcg ccggtgacga cgaccccccg gccggggttc tcgtcggcnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg tgctgtcctg tggggtgctg tggtcggtca
     5221 tctcgcggtc aaccctccgt cgacggggat ggtgatgcca gtgatgaaac ctgcatcgtc
     5281 actggcgagg aaggccaccg ttccggcgac ctccccgggg gttcccggcc ggccgagagg
     5341 cacggtggcg aggaaggagt ccatgaggtc ggcgatgctc gcgccgggct cgcggccgaa
     5401 gaaggtcggc agcatcgggg tgtcgaccgg cccgggcgcg acgacgttga cccggatccc
     5461 gaccggggcc ccgacgagcg cgagtgagcg ggcgaaggcc acgagcgcgc ccttggtcaa
     5521 cgagtagagc gggctgaacg ggctgccgac gagggccgcg gtcgaggccg tgaaggtcac
     5581 cgacgccttg ccttcggcgc gcgagagcag cggccaggcc agcgacgcac cgaaggtcgc
     5641 cgacttgatg ttaacggcga cagcatcgtc gaactcgttc tcggagaagt caattcctcc
     5701 ggcgccaggc gcgccgacct ggtggtggaa cacgtgcaga acgccgtggt cggcctcgac
     5761 ccgctcgaaa agcgcgcgga gcgcgtcgac ggaggtggcg tccacgacat gggggatcgc
     5821 ggcatacggg atggcagcgc tgccgctcgc cgtgatggcg ctggcgacct cgtatgccgc
     5881 ctgctcgcgc gcatcggcaa cgtagacgat cgcgccctcg gaggccaggc gctcgctgat
     5941 cgcgcggccc atgccggagc cgccgccgac gaccacggcc acgcggccga ccaaccggcg
     6001 gcacgggggt ccgctggtgg ggctgggcgt agggcgggtc gccgtcaggg ccgggtcctc
     6061 gggcgcgctc actcgccgcc cccggcgctg ctgcccgtcg tgttgcggcc gccgccgggg
     6121 atcttgcggg agtcgtggct gaccgcgagc gcgaccggct gggccttgaa gatgacccgg
     6181 ttggggctgt cgagcagctt catccacggg gcttcgttgc ccggctgcag tcgcttggtg
     6241 aactcgtcga tgaaccacgc cttggtgcgc cggtcgcggt ggacggtgac gacgcagcgg
     6301 tatgccgcca tctgtcgccc cggcaggtcg gtccccctgt tgctgacgac gatcgtcgcg
     6361 cgcgggtcct tggcgatgcc cttggtctga ccgcgacctt cgaccgcggt gagccagaag
     6421 acgccgtcga tgtggatgta ggtcatgaca accccgctgg gccacccggc ctcactggtg
     6481 aagacgaagg tgcactcgga ctgcgtctcc aggagctcgg cgcgctcctc gtgccgcagc
     6541 cccagaccct ggagggcctc gaggtcatcg atcccgaccc gtcccatgtc cgcacctcct
     6601 ccacgttgtt ctctagccgg ggtgatgcct agcccgcgct ttgatgatat tcatgttcat
     6661 gatttgcggt caagggctcc ccgtggccaa gagcggttgt gtatgctaac gataatcaga
     6721 gatcgggccc gtgcgggctc ccgaggaggt cgtcgtgagc aacgcggtca tcgtcgaagc
     6781 ggtgcggacg cccatgggtc gcggcaaggc gggcggcgcc ctcgccgacc tgcatccggt
     6841 tgacctgctc gcccagactc tcacggcgtt ggtcgctcgg acgggcatcg accccggcat
     6901 cgtcgacgac gtcatcatcg gctgcgtcgg gcagaacctc gagcagtcgg ccaccccggg
     6961 ccggcaggcg tggctggcgg ccgggttccc cgagcacgtc ccatcggtga ccatcgagcg
     7021 caagtgcggc tccgggcagc aggcgatcga gttcgccgcg gcagggatcg tcgcggggct
     7081 ctatgacatc gtcatcgccg gcggggtcga gtcgatgagc cgggtgccga tcatgtccgc
     7141 gcggatgggc gccgacccgc acggccccgg cgtccgggcg cgctaccccg gcctcgtccc
     7201 gcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcggcg acgaccccca
     7321 actcatgctc accggcccga tcgcggcgac gacgaaagtc ttggctcggg ccgggctcac
     7381 gctgcgcgac atcgacacgg tcgaggtcaa cgaggccttc gcatcggtgc cgctcgcgtg
     7441 gctggccgag cacccctatg acgaggagcg gctcaacccc cgcggcggcg cgatcgccct
     7501 ggggcacccg ctcggcgcct ccggggcccg gatcatgacg agcatgctcc accacctgga
     7561 gcagaccggc ggccgctacg gcctgcagac gatctgcgag gccggcggca tggccaacgc
     7621 gaccatcctc gaacgcctcg gctgacccgc gaaccgggct gaccggcata cctctgccgt
     7681 gggcccccac cacccaagcc ccccttccgc cccggaacca aggagactgc ccgcatgcac
     7741 ctggagaact cgtccgccgt cgtcaccggt ggggcctccg ggctcggcct ggccacggtc
     7801 caggccctgc tcgcccgcgg ggtcctcgtg accatccttg acctgccgac ctcccagggg
     7861 gcgcaggtcg ccgaatccct cggcgctgca gcctcattcg cgcctggtga cgtgcgcgac
     7921 gacgaggcgg tcgccgctgc cgtggccctc gccgacggtc gggcgccgct gcgcacgctc
     7981 gtccactgtg cgggtcgcgg cggcacggtg cgggtgctcg acaaggctgg ggcaccgggc
     8041 gactacgacc tctatcgcga gatcgtcgcg gtcaacctct tcgggacgtt caacgtgctg
     8101 cggcacgcgg cgtcggcgat ggcgacccac gagccgatcg acggcgagcg cggcgtctgc
     8161 gtgctcacgg cgtcggtggc ggcgttcgag gggcagatcg gccaaatccc ttacgcctca
     8221 gccaaagccg gtgtcgtcgg catgacgatc gtcgccgcgc gcgacctcgc gcagcggcag
     8281 atccgcgtgt gcacgatcgc accgggggtg ttcgacacgc cgatcctcag ccgcttctcg
     8341 caggacatca aggacgggct ggcggcgcag gtcccccacc cgtcgcggct gggtcggccc
     8401 gaggagtatg ccgccaccgc gatgcacatc gtcgacaacg cgatgctcaa cggcgagacg
     8461 attcgtcttg acggagcgat ccggatgagc ccccgatgac cgagggtctg ggcggctctc
     8521 tgggggaggg tggcgcgatg gggagcccgg tgcacaccga ggtgcgcggc ggcgtgctgc
     8581 tcatcgtgct caaccgcccg gagatcaaga acgcgatcaa cacggagacg gccgaggcgg
     8641 ttgccgcggc gctcgatcgg ctcgacagcg acccggccct cacgtgtggg gtgctgaccg
     8701 gtgcgggcgg aacgttctgc gcggggatgg acctcaaggc gttccttgcg ggcgagcggc
     8761 cgtcggtgcc ggtgcgtggg tttgccggga tcgtcgagcg gccgtccgac aagccgctcg
     8821 ttgcggccgt cgagggtgcc gcggttgcgg gtgggttcga gatcgcgctg gcctgtgacc
     8881 tggttgtcgc cggtgagagc gctcggttcg ggttgccgga ggtgcgtcgt ggcctggtcg
     8941 ccgctggtgg cgggctgttg cggctggccg agcggatccc gacggcgatc gccatggagt
     9001 gggcgctcac gggtgccttg gtcggcgcgc agcgggcgtt cgaggt
//
LOCUS       DAORPW010000088         9001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1846_length_9001_cov_661.142186, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000088 DAORPW010000000
VERSION     DAORPW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1846_length_9001_cov_661.142186"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1427
                     /gene="radA"
                     /locus_tag="PLB86_08225"
     CDS             36..1427
                     /gene="radA"
                     /locus_tag="PLB86_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRC65160.1"
                     /translation="MATNNSVRARERGTAYRCSECGWSTVKWVGRCGECQAWGTVGEA
                     GDGRPRTTAPARAVPPPARIGEVDRTAAVARPTGVSEFDRVLGGGLVPGAVVLVAGEP
                     GIGKSTLLLDVAARAARSGRDVLYVSGEESAAQVRLRAERIEAMASSLFLAAETDLAT
                     ILGQIEAVTPDLVIVDSVQTIASAQVEGAAGNVAQVREVAASLIHVAKTRGIAVLLVG
                     HVTKDGSIAGPRLLEHLVDVVVQFEGERHSRLRLVRAAKNRYGPTDEVGCFDLSDVGI
                     VGLPDPSGLFLSSREVAVSGTCVTVTLEGRRPLVAEIQGLVTASTLPTPRRATHGLDA
                     SRIAMILAVLQQRAGAQIGGKDAYVATVGGVKVTEPAADLAIALAVASALRDEPFPSD
                     GIAFGEIGLAGEIRSVAGMPRRLAEAARLGFRRAWVPRGVAGSGPMPDGIRIIEVSQV
                     WEAVGRALSSSSR"
     gene            1507..2583
                     /gene="disA"
                     /locus_tag="PLB86_08230"
     CDS             1507..2583
                     /gene="disA"
                     /locus_tag="PLB86_08230"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HRC65161.1"
                     /translation="MERTDDDLLIATLAAVAPGTELRDGLERILRSGTGALIVIGSDK
                     SVEAICSGGFRIDAEFSATRLRELTKMDGAVVIDKDVAKIIRANTQLVPDSRIETSES
                     GTRHRTAERVAKQTGLPVISVSKSMQTIALYIGGRRHVVENSASILSRANQALQTLER
                     YKGRLDEVTGTLSALEIEDLVTVRDVAAVLQRLEMVRRISEEIHGYAVELGTDGRLLS
                     LQLEELVGGLGNDRELVIRDYLPVGKGAKTVDAAMADLVGLSATELLDLAAGARAVGF
                     AGPADGLDTSVSPRGYRLLTRVPRLPGAIIDRLVEHFDSLQRLLAANIEDLMAVEGVG
                     ESRARAVREGLSRMAESSILERYV"
     gene            2588..>3042
                     /locus_tag="PLB86_08235"
     CDS             2588..>3042
                     /locus_tag="PLB86_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRC65162.1"
                     /translation="MPHPSPVRLARVHADVLRWYAVHARPLPWRAPGTSPWGVYVSEI
                     MAQQTPVARVEPVWREWLTRWPTPADLAAATPGDAVRAWGRLGYPRRALRLHDAARVM
                     VDRHDGQVPTELTDLLALPGVGAYTAAAVACFAHGIPEAVVDTNVRRVLA"
     assembly_gap    3043..3142
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3143..3358
                     /locus_tag="PLB86_08240"
     CDS             <3143..3358
                     /locus_tag="PLB86_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRC65163.1"
                     /translation="GPARRGQAWVGTDRQARGALLQALRDAPGTVRSMTLEAVWPDPI
                     QRARCLDGLVADGLVEPLAGQRFRLPG"
     gene            3479..>4662
                     /locus_tag="PLB86_08245"
     CDS             3479..>4662
                     /locus_tag="PLB86_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRC65164.1"
                     /translation="MGRHQTDAPPVRPRWVIPLVAFAVAVGGGGGAWWAISRAGGPST
                     EAGPASSASGGTAAAVTTPCTSSVAVQVAVAGPLVPAVRTAADLATASGACATYAVTA
                     QSAPTTAAAIAGGTGPNVWIPDSSAWVADLTAKVPNAVTAGQSVALSPLVVAIPKALA
                     TSVTSAPTWAQLVAFTLPVQFADPTASASSRLALRSAYAALGTSSEARIALGAAMIRL
                     SRTKVASEDVLYAQATAGATPASAFPAEEAAVAAYNAANAGTRLVAAIPAGGTSAFDH
                     PFVTVGAPSASVKAGADALLAALQSPAGKAAVAAAGFRSGPTDAAPKVDGLIGGAPTY
                     LPEPPPEAATALARSWAAVGTEMRMLAVVDTSGSMKDPAGSSTRMALAHQAGLVALSA
                     FPP"
     assembly_gap    4663..4762
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4796..5602
                     /locus_tag="PLB86_08250"
     CDS             4796..5602
                     /locus_tag="PLB86_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HRC65165.1"
                     /translation="MRAYLWTYWLLARAGFRRQATYRMALAAGIFTNSVFGVVRASIL
                     VGAVAGAGAAVAGYDALAVSSYAWWGQVLLGSVSMWGWSEVADRVRTGDIAADFLRPV
                     PPYLTYLAVDLGRAALALLARGAPMLLIGALYSGITFATGAVEWVLGLTAVALAVAVG
                     FLGNYLVNCAAFWLVEIRGLLMAYSVVATLLAGLYVPVHWFPEWLRTVAAATPFPSLI
                     QTPIDILSGYAAGPAAVRAVAVQVGWVLALGVAGALATAAGRRRLEVQGG"
     gene            5595..6398
                     /locus_tag="PLB86_08255"
     CDS             5595..6398
                     /locus_tag="PLB86_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HRC65166.1"
                     /translation="MASGFTAYRAVLRSRVRAQQGYRVSFATDLFATVLTGLAELLEV
                     YVIFHNTPRLGGLDFDAALLVFALANLTFAAADLLVGHCDRLPTYVREGRIDVFYLRP
                     LSILGQLMTSEVSLRRLGRISVAVVVLGIALVRGEVAPTPTTVGMLALSIVCGTALMA
                     GLFVAVAGLQFWLVDGREFANAFTYGGAYAAQQSSLVFPDPVRIFFTFVVPAAFTAYL
                     PTLVILGEPGGDFADPALAWWLPAATAWVWGVALLTWRHGVRHYQGAGG"
     gene            6401..7390
                     /locus_tag="PLB86_08260"
     CDS             6401..7390
                     /locus_tag="PLB86_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC65167.1"
                     /translation="MSVDPVRPVSASGPVSAPIVAVEGLTRVFRRRGAPALTAVDAMT
                     FRIAPGEAVGYIGANGAGKSTTIKMLTGILAPTSGTARTVGLDPMRDRRELARRVGVV
                     FGQRSQLWWDLPLRESFDLLAAIHRLDRLRATRRTGQLVELLDLGPTLAQPVRSLSLG
                     QRMRAEVAAALLHEPELVILDEPTIGLDVLSKARLREFLIQTRREHGTTLLLTTHDMG
                     DIERLCDRVLVVDHGRLAFDGTLAGLAGTVGARRVLVVDLDRPSEPLHGIPHTQWLGA
                     EGEGVRQRLAFDPADTTAAAVLAAVSERVTLRDLSIEEPDIEDVVRRIYSASS"
     gene            complement(7416..7916)
                     /locus_tag="PLB86_08265"
     CDS             complement(7416..7916)
                     /locus_tag="PLB86_08265"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HRC65168.1"
                     /translation="MTGLSTRPARTSDVRAIREIVAPLVAKRRLVAKDAVAYYEGIQQ
                     FRVVQDGDTVIGCGALHVMWEDVAEVRTLAVAAAYLGRGAGHLLLEALIEDARTLGVQ
                     RVFCLTFETTFFADHGFQVIEGQAVPPDVYAEVVRSYDEGVAEFLDLERVKPNTLGNT
                     RMLLTL"
     gene            complement(7942..8361)
                     /locus_tag="PLB86_08270"
     CDS             complement(7942..8361)
                     /locus_tag="PLB86_08270"
                     /inference="COORDINATES: protein motif:HMM:NF039679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6188 family protein"
                     /protein_id="HRC65169.1"
                     /translation="MADVGLEPLLREEGAHYVLPLVGREVDQLCIDYAVTLVVDGTDT
                     IRLESPFTLHVDGTTQVVDPERLETVAAVLQLFRAVVTRAEAGKDGSLTIDFDSDRWL
                     RAEAIEDGEAWEIGGGLPPVTPSYFIAAQSGGGVRLS"
     gene            complement(8409..>9001)
                     /locus_tag="PLB86_08275"
     CDS             complement(8409..>9001)
                     /locus_tag="PLB86_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC65170.1"
                     /translation="MVDFKNTVIIMTTNLGTRDITKGSLGFSAGPDTRSEYERMKNKV
                     TDELKNHFRPEFLNRVDDVIVFPQLTQDEIVAIVDLEIAKLDKRLRDKDMGLELTMAA
                     KALLAKKGYDPVLGARPLRRTIQREIEDMLSERILYGELRSGEFVLVDATGDEKDSTF
                     TFTGSLRKDTIPDIPDVPPIDEVGGPQDAQASASGT"
BASE COUNT         1178 a         3208 c         3017 g         1398 t
ORIGIN      
        1 acccgctgtc ggaccctggc cctagggtca cgatcatggc gaccaacaac tcagtgcgag
       61 cccgcgagcg aggcacggca taccgatgct cggagtgcgg ctggagcacg gtcaagtggg
      121 tgggccgctg cggcgaatgt caggcctggg ggacggtggg cgaggcgggc gatggtcggc
      181 cacgcacgac tgcccctgcc cgcgcggtgc cgccacccgc gcggatcggt gaggtggacc
      241 gcaccgccgc tgtagccagg ccgaccgggg tcagcgagtt cgatcgggtg ctcgggggcg
      301 gtttggtccc gggagcggtc gtgctcgtgg ccggcgagcc ggggatcggc aagtcgaccc
      361 tgttgctcga tgtcgccgcc cgcgcggccc gatccggacg agacgtcctc tacgtcagcg
      421 gtgaggagtc tgccgcccag gtgcggctgc gcgccgagcg gatcgaggcc atggcctcgt
      481 ccctcttcct cgccgccgag accgacctcg ccacgatcct cggccagatc gaggcggtca
      541 cacccgacct cgtcatcgtc gactcggtgc agacgatcgc cagcgcccag gtggaggggg
      601 ccgccggcaa cgtcgcccag gtccgcgagg tcgccgcgag cctcatccat gtcgccaaga
      661 cccgaggcat cgccgtgctg ctcgtcgggc acgtcaccaa ggacgggtcg atcgccggcc
      721 cgcgcctgct ggagcacctc gtcgacgttg tcgtccagtt cgagggcgag cggcactccc
      781 ggttgcgcct ggtccgagcc gccaagaacc gctacggccc gaccgacgag gtgggctgct
      841 tcgacctgtc cgacgtcggc atcgtcgggc tccctgaccc gagcgggctg ttcctctcca
      901 gccgcgaggt agccgtctcg gggacctgcg tcaccgtcac cctcgagggc cgccgccccc
      961 tcgtcgccga gatccaaggc ctggtcaccg cctcgacctt gcccacccct cgccgagcga
     1021 cccacggcct ggacgcctcc cggatcgcga tgatcctcgc ggtcctgcaa cagcgcgcag
     1081 gagcccagat cggcggcaag gacgcctacg tcgcgaccgt tggcggggtc aaggtcaccg
     1141 agcccgcggc cgacctcgcg atcgccctcg cggtcgcctc agcgctgcgg gacgagccgt
     1201 ttccctccga cgggatcgcc ttcggcgaga tcggcctcgc gggggagatc cggtcagtcg
     1261 cgggtatgcc gcgtcgcctg gccgaggccg cgaggctcgg cttccgccgc gcctgggttc
     1321 cccgaggagt ggccggcagc gggccgatgc cggacggcat acggatcatc gaagtgagcc
     1381 aggtgtggga ggcggtgggc cgggcactgt cctcctcatc gcgctgagac gcggcggcct
     1441 ccgaccgtgt cgtccgtgtc ctgcgtcgac cccgcccccg acccagccac agctagactc
     1501 cgagccgtgg aacgaaccga cgacgacctg ctcatcgcga cgctggctgc cgtcgcgccg
     1561 ggcacggagc ttcgcgacgg gcttgagcgc atcctgcgca gcggcaccgg cgcgctcatc
     1621 gtcatcggca gcgacaagtc ggtggaggcg atctgctcgg gcgggttccg gatcgacgcc
     1681 gagttctcgg cgacccggct gcgggagctc accaagatgg acggcgccgt cgtcatcgac
     1741 aaggacgtcg ccaagatcat ccgcgccaac acccaactcg tccccgactc ccggatcgag
     1801 acctccgagt ccggcacccg gcaccgcacg gccgagcggg tggccaagca gaccggcctg
     1861 cccgtcatct cggtgagcaa gtcgatgcag acgatcgccc tctacatcgg cgggcgacgg
     1921 cacgtcgtcg agaactccgc gagcatcctc tcccgcgcca accaggccct gcagaccctc
     1981 gaacgctaca agggccgcct cgacgaggtc accggcaccc tgtcggcact ggagatcgag
     2041 gatctcgtca ccgtccgcga cgtcgccgcc gtcctgcagc gcctggaaat ggttcgccgg
     2101 atcagcgagg agatccatgg gtatgccgtg gagctcggca ccgacggccg cctgctcagc
     2161 ctgcagttgg aggagctcgt cggcggcctg ggcaacgacc gcgagttggt gatccgcgac
     2221 tacctccccg tgggcaaggg cgccaagacc gtcgacgctg cgatggccga cctcgtcggc
     2281 ctgtcggcta ccgagctgct cgacctggcc gccggtgctc gcgcggtcgg cttcgccggc
     2341 ccggccgacg gcctggacac ctcggtctcg ccgcgcggtt atcgcctgct cacccgggtg
     2401 ccgcgtctgc cgggagcgat catcgaccgg ctcgtcgagc acttcgactc gctccaacgc
     2461 ttgctcgcgg ccaatatcga ggacctcatg gcggtcgaag gggtcggtga gagcagggcc
     2521 cgtgcggtcc gcgagggcct gtcccggatg gccgagtcga gcatcctcga gcgctacgtc
     2581 tgaccgcgtg ccgcacccct cccccgtccg gcttgcccgg gtgcacgccg acgtccttcg
     2641 ttggtatgcc gtccacgcca gaccgctgcc ctggcgggct cccgggacga gcccttgggg
     2701 ggtctacgtc agcgagatca tggcgcagca gaccccggtg gcccgcgtcg agccggtctg
     2761 gcgggagtgg ctgacccgct ggcccacacc cgccgacctc gcggcagcga cacccggtga
     2821 tgcggtgcgg gcctgggggc ggctgggcta cccgcgccgc gcactgcgac tgcacgacgc
     2881 cgccagggtg atggtggatc ggcacgacgg tcaggtgccg accgagctga ccgacctgct
     2941 cgccctgccc ggcgtgggcg cctacaccgc cgccgccgtg gcctgcttcg cacacggcat
     3001 acccgaggct gtcgtcgaca ccaacgtccg gcgcgtgctc gcnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnggcccggc ccggcgtggt caggcctggg tcggcaccga
     3181 caggcaggcc cgcggcgctc tcctgcaggc gctgcgagac gcgcccggca ccgttcgctc
     3241 gatgacgctg gaagcggtct ggcccgaccc gatccaacgg gctcgctgcc tggacggact
     3301 cgtcgcggac ggcctggtcg agccgttagc cggccagcgg ttccgcttac caggctgacc
     3361 gctcgcaccg gatcggcagg caactatcgg gtaggtgctc cggacaacta gcgtgagccc
     3421 cgcggttgct cactctcccc acctctgccc actctcccag ctcggaggac acacacgcat
     3481 gggtcgccat cagaccgacg ctccgcccgt ccggcctcga tgggtcattc cactggtcgc
     3541 gttcgccgtg gctgtcgggg gcggaggcgg cgcgtggtgg gcgatctccc gcgctggagg
     3601 cccgtccacc gaagcggggc cagcgagcag cgccagcggg ggcaccgctg ctgcggtgac
     3661 caccccgtgc acgtccagcg tcgcggtcca ggtcgccgtt gcggggccgc tggtccccgc
     3721 ggtgcgcacc gccgccgacc tcgccacggc ctccggggcc tgcgcgacgt atgccgtgac
     3781 cgcccagtcc gcgcccacca ccgccgccgc catcgccggg ggcacggggc ccaacgtctg
     3841 gatccccgac tcctccgcct gggtcgccga cctcaccgcc aaggtgccga atgccgtcac
     3901 cgcgggtcag tccgtggccc tgtcgccgct cgtcgtggcg atccccaagg ccctcgccac
     3961 gtcggtgacc tcggctccga cgtgggccca actcgtcgcc ttcaccctcc ccgtccagtt
     4021 cgccgacccc accgccagcg ccagttcgcg gctggccctg cgctcggcgt atgccgccct
     4081 cggaaccagc agcgaagccc gcatcgccct tggcgccgcg atgatccggc tgagccgaac
     4141 caaggtcgcc tccgaggacg ttctctatgc ccaggcgacc gcaggcgcca ccccggcgag
     4201 cgccttccct gccgaggaag ccgcggtcgc ggcatacaac gccgccaatg ccggcacgcg
     4261 cctggtcgcg gcgatccccg ccggtgggac cagtgccttc gaccacccgt ttgtcacggt
     4321 cggcgcgccg tcagcctcgg tgaaggccgg ggccgacgcc ctgctcgccg cgctccagag
     4381 cccggcaggt aaagcggccg tcgcagcggc cggcttccgc agcggtccca ccgatgccgc
     4441 gcccaaggtc gacggtctca tcggtggggc cccgacctac ctccccgaac cgccccccga
     4501 ggcggcaacc gccctggccc ggtcctgggc cgccgtcggc accgagatgc gcatgttggc
     4561 agtcgtcgac acgtcgggct cgatgaagga cccggcgggg tcgagcacgc ggatggcgct
     4621 ggcccaccag gccgggctgg tcgccctgtc ggccttcccg ccnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nngcgcggca taccccggcg tagggtcgcg agtttgtgcg
     4801 cgcctacctt tggacctact ggctgttggc cagagccggg ttccgtcggc aggcgaccta
     4861 tcggatggcg ctggccgcgg ggatcttcac caactcggtc ttcggggtcg tccgagcctc
     4921 gatcctcgtg ggcgcggtgg cgggagcggg tgccgccgtg gccggttacg acgccctagc
     4981 cgtctcgtcc tacgcctggt ggggtcaggt gcttctcggc tcagtgtcca tgtgggggtg
     5041 gagcgaggtc gccgaccggg tccggacggg tgacatcgcc gccgacttcc tgcggcccgt
     5101 gcccccctac ctcacctacc tcgccgtcga cctcggccga gccgccctgg cgttgctcgc
     5161 ccgtggggcg ccgatgctcc tgatcggcgc cctctattca gggatcacct tcgccaccgg
     5221 cgccgtcgag tgggtgctgg ggctgaccgc cgttgcgctg gccgtcgccg tcgggttcct
     5281 cggcaactat ctcgtcaact gtgccgcctt ctggctggtg gagattcgcg gcctgctgat
     5341 ggcctactcg gtcgtggcga cgctcctggc cggcctctac gtgccggtcc actggttccc
     5401 cgagtggttg cgcaccgtcg cggccgccac ccccttcccg tcgctcatcc agaccccgat
     5461 cgacatcctc agtgggtatg ccgcggggcc cgcggccgtc cgcgccgtcg ccgtgcaggt
     5521 cggttgggtg ctcgcgctgg gcgtcgccgg tgcgctcgcc actgcggccg gacgacgccg
     5581 gttggaggtc cagggtggct agcgggttca cggcataccg agcggtcctg cgttcgcggg
     5641 tccgcgctca gcagggctac cgcgtgtcgt tcgcgaccga cctcttcgcc accgtgctca
     5701 ccggtttggc cgagctcctc gaggtctacg tcatcttcca caacaccccg cgcctcggtg
     5761 gcctggactt cgatgcggcc ctgctcgtct tcgcgctggc caacctcacc ttcgctgccg
     5821 ccgacctcct ggtggggcat tgcgaccgac tgcccaccta cgtgcgggaa ggccggatcg
     5881 acgtcttcta cctgcggccc ctgtcgatcc tcggtcagtt gatgacgagc gaggtgtcgc
     5941 tgcgtcggct cgggcggatc tccgtggccg tcgtcgtgct cggcatcgca ctggtccggg
     6001 gcgaggtcgc gccgaccccc acgacggtcg ggatgctcgc cctgtcgatc gtctgcggca
     6061 ccgcgctgat ggccggcctc ttcgtcgccg tggccgggtt gcagttctgg ctcgtcgacg
     6121 gacgcgagtt cgccaacgcg ttcacctacg gcggcgcgta tgccgcgcag cagagttcgc
     6181 tggtcttccc cgatccggtg cggatcttct tcacgttcgt cgtgcccgca gcgttcacgg
     6241 catacctgcc gaccctggtg atcctcgggg agccgggcgg cgacttcgcc gatccggcgc
     6301 tggcgtggtg gctgcccgcc gcgaccgcgt gggtctgggg ggtcgcgctg ctgacctggc
     6361 ggcacggcgt gcggcactat caaggcgcag gaggatgagc atgagcgtcg atcccgttcg
     6421 gccagtgtcg gcatcagggc cggtgtcggc accgatcgtg gccgtcgagg gcctcacgag
     6481 ggtgttccgc cgtcgcggcg ctcccgcgct caccgccgtc gacgcgatga ccttccggat
     6541 cgcccctggc gaggcggtcg gctatatcgg agccaatggg gctggcaagt ccacgaccat
     6601 caagatgctc accgggatcc tcgcgcccac ctcaggcacg gcgcgcaccg tcggcctcga
     6661 cccgatgcgc gatcgccgcg agttggcccg gcgcgtcggg gtcgtgttcg ggcagcgctc
     6721 tcagttgtgg tgggacctcc ctctgcggga gtcattcgac ctgctcgccg cgatccaccg
     6781 gctcgaccgg ttgcgcgcca cccggcggac gggccaactc gtcgagttgc tcgacctcgg
     6841 gccgacgctg gcgcaaccgg tgcggtcgct gtcgctgggc caacggatgc gcgccgaggt
     6901 tgccgctgcg ctgctgcacg agccggagtt ggtcatcctc gacgagccga ccatcggcct
     6961 ggacgtgctg tccaaggccc gactgcggga gttcctcatc cagacccgcc gcgagcacgg
     7021 cacgaccctg ctcctgacga cccacgacat gggagacatc gagcgcctct gcgaccgggt
     7081 gctcgtcgtc gaccatgggc ggctggcctt cgacgggacg ctggctggcc tggccggcac
     7141 cgtcggggcc aggagggtgc tcgtcgtcga cctggaccgg cccagtgagc cgctgcacgg
     7201 cataccccac acgcagtggc ttggcgccga aggtgagggc gtgcgacagc ggttggcctt
     7261 cgacccggcc gacacgacgg cggcggccgt cctcgccgcg gtctccgagc gggtgacgct
     7321 acgcgacctg agcatcgagg agccggacat cgaggacgtc gtccggcgga tctactccgc
     7381 gtccagttga ccgtcagggc gcctggaggt gccggtcaga gcgtcagcag catccgggtg
     7441 ttgccgaggg tgttgggctt gacccgctcg agatcgagga actccgccac gccctcgtcg
     7501 taggaccgga ctacctcggc atacacatcg ggcgggacgg cctgcccctc gatcacctgg
     7561 aagccgtggt cggcgaagaa cgtcgtctcg aaggtgaggc agaagacccg ctgcacgccc
     7621 aaggtccgcg cgtcctcaat gagcgcctcc agcagcaggt ggccggctcc gcggcccagg
     7681 tatgccgccg ctaccgcgag cgtgcggacc tcggcgacgt cctcccacat gacgtgcagg
     7741 gcgccgcagc cgatcaccgt gtcgccgtcc tgcacgacgc ggaactgctg gattccctcg
     7801 tagtaggcga ccgcgtcctt ggccacgagt cgtcgcttcg ccacgagggg ggcaacgatc
     7861 tcgcgaatgg cccgcacgtc gctcgtgcga gccggtcggg tgctcaggcc ggtcacgaca
     7921 gacgatcgta aggggtccgg ctcaggacag gcggacgcct ccgccggact gggccgcgat
     7981 gaagtagctc ggggtgaccg gcggaaggcc gccgccgatc tcccaggcct cgccgtcctc
     8041 gatggcctcg gcacgcaacc agcggtcgct gtcgaagtcg atggtcaacg acccgtcctt
     8101 gcccgcctcg gcgcgcgtga cgacagcccg gaacaactgc agcaccgcgg cgaccgtttc
     8161 cagtcgctcg gggtcgacga cctgggtggt cccgtccacg tgcaacgtga acgggctctc
     8221 caggcggatg gtgtcggtgc cgtcgacgac aagcgtcacg gcgtagtcga tacagagctg
     8281 gtcgacctcg cgcccgacga ggggcagcac ataatgcgcc ccctcctcgc ggaggagggg
     8341 ttcaagcccc acgtcggcca tgacctgagg gtacgacacc caccgacctc ctgagtgctt
     8401 ctccggcttc aggttccaga agcgctggcc tgcgcatctt gcggcccacc gacctcgtcg
     8461 atcggaggaa cgtccggaat gtcaggaatc gtatcctttc gcagcgaccc ggtaaacgtg
     8521 aaggtggagt ccttctcgtc gccagtggca tcgaccagga caaactcacc gctacggagc
     8581 tctccgtaga gaatcctttc ggagagcatg tcctcaatct cacgctggat cgtgcgccgc
     8641 agaggccgcg caccgagcac cgggtcgtag cccttcttgg ccagcaaggc cttggcggcc
     8701 atggtcaact cgaggcccat gtccttgtcg cgcaagcgct tgtcgagctt ggcgatctcg
     8761 aggtcaacga tggcgacgat ctcgtcctgg gtgagctgcg ggaagacgat gacgtcgtcg
     8821 acacggttga ggaactcggg ccggaagtgg ttcttcagct cgtcggtgac cttgttcttc
     8881 atccgctcgt attccgagcg ggtgtccggt ccggccgaga agccgagcga ccccttggtg
     8941 atgtcccggg tgccgaggtt ggtcgtcatg atgatgacgg tgttcttgaa gtcgaccatc
     9001 c
//
LOCUS       DAORPW010000089         8841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1900_length_8841_cov_839.790121, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000089 DAORPW010000000
VERSION     DAORPW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1900_length_8841_cov_839.790121"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PLB86_08280"
     CDS             <1..705
                     /locus_tag="PLB86_08280"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC65171.1"
                     /translation="AGLPLLVAIVCVIPLVVGVAVLLERMTIAPVKKMTPLTSIILTL
                     GVSTAVKAVMLLIWGPEAKGLPNFPGSDMTLGGVSIRSQELWVLGIGALVAYAVIWFY
                     EHTVRGKSLRACAEQPIAARLVGISPRTATVVAFAVAGFTGAVAGIVGSPIYLSSWQS
                     GLSLGLKGFVAATLGGLTSLRVAMIGGMALGVLENLVAGYLSSGYRDAVAFVVLVLVL
                     LFRPNGLAGKTSAVRV"
     gene            702..2606
                     /locus_tag="PLB86_08285"
     CDS             702..2606
                     /locus_tag="PLB86_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HRC65172.1"
                     /translation="MNPLFKRQIPFIALLVGLLVFGAAAPQQMVDVGIWALIYGFAAI
                     GLSLLMGLAGQVSLGHAAFIAVGAYTQALLVAKANLNPLLAAPISVVAAMLVALLVGL
                     PLLRLRGHFLALATLGLGIIVGVFVTEQEFTGGTSGIFGLPNISFGGRVYDTSQEYFL
                     LLTPVVVIGLWLASNLVHSRTGRALSAVNDSEVAAECLGVDTFALRLRVFVVSAGYAG
                     LAGVFYTHWLTIVNPSVSHFELSVKILLMVVLGGLGTVWGALIGAVAVQLLDDGLRGL
                     IPILIPGAKGEIQLIGFGVVLVLVIILMPGGLAQLWARLMSAVRQRKHPIVRGEHETP
                     VSQEEQRALLEESLKLLARAEHMPAGQTILAIRGLTKRYGGVTALDNLDLDVKAGEIM
                     ALIGPNGAGKTTAFNMITGVMVPTEGSVLLQGTEVAGKRPHIAAEIGATRTFQNLQTF
                     KSTTVIGNVKVARHLRSKAGLVRGMLMLDRTEERLIDQASQAAIDAMGLTSLADKQIA
                     DLAFGKQRQVEVARALALEPSVLLLDEPMAGLSGPERDSLSWLLRRVKASGVTVLLVE
                     HDVAAVMALADRVAVLDDGKLISLGTPAHVTSDPKVIAAYLGEEDDQSGGPATGAPAA
                     TSGAAAGGLA"
     gene            2603..3397
                     /locus_tag="PLB86_08290"
     CDS             2603..3397
                     /locus_tag="PLB86_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65173.1"
                     /translation="MTTDHIPRIGLRVEAIDAAYDRLQVLWDVSLHVEPGELLALVGS
                     NGAGKTTVLRSVSGLLKPVRGRVLLGDTDITSTSAEKIAELGLAHVPENRLVFPSLTV
                     EDNLVLGGYVRRRDGRYAVRRDEALHLFPRLRDRINLLAGALSGGEQQMLAMARALMS
                     DPSVIILDEPSLGLAPKVVTEIMSALGHLRDEKGLAVLLVEQNVRAAFKIADRVLVMD
                     RGRVVAEGTPAELASDERVRAAYLGGSSTSSSGVAAPVTRSGGTSA"
     gene            3448..4407
                     /locus_tag="PLB86_08295"
     CDS             3448..4407
                     /locus_tag="PLB86_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC65174.1"
                     /translation="MSKPFASSTDLAAKTETLEVLADGVYALTAEGDPNVGAIEGEDF
                     LVGFEARATPAAARDWIAKLREHTDKPFRYLVLSHYHAVRVLGAEAFEADEIIAHERT
                     RFLVQERGMQDWASEYGRMPRLFKEPEGIKGLTWPTLTFSDEMTIPLGGDRGDLVLKY
                     LGRGHTEGDIVAWLPKHKIMFAGDLVEAKAALYTGDAFHQEWASTTLDAVKAMGAEAI
                     VGGRGEIPRGEAAVNAAIEQSRGFITSIIDNVRATQAAGGTLKQAFDACHAALSPTYG
                     HWPIFEHTLPFDVARTWEELQGVERPTIWTAERDQEIWAQLQG"
     gene            4459..6078
                     /locus_tag="PLB86_08300"
     CDS             4459..6078
                     /locus_tag="PLB86_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRC65175.1"
                     /translation="MSQDHGTEGRGRVLVIGNGPVGQTAALCLARWGIPVLVLDSRPE
                     RDPIGSKALCQQRDVLDLWDACGAGAVATEGLTWSRARTFYKDKELFCWEIPDPGRSP
                     LPPFVNISQARSEAILDEAIAAQPLIEVWWGTEVVGVAGGKSDSDGLATVVCRSGFGE
                     FEVAGEYAVVCAGSKGGPLREALGVTFPGRTFDDSFLICDIETTLKGWENERRFYFDP
                     EWNPGRQVLIHPCPNSIFRIDWQVDTDFDLAAAQADGSLDKRIRKVIGKKRDYRIDWV
                     SVYRFHARSADRFRVGRFLLAGDVAHLVAPFGARGLNSGVPDVENAAWKIAFAMKGWA
                     GPGLVGSYESERQAAARENLAVTAATMDFLVPQTKKAKHRRKEILKRARKDKKVIPLV
                     DSGRLAEAFWYVDSPLTTPDPSRFWPGRPPRGQAPTPVPGVILPDAVTASAGHGKSRV
                     RELAREGLLILVGEAVNTHEVEGLLSGVVPAGLPVRVLHFSDLGDEDVVATGLQAVAE
                     ELWLIRPDAHTAACVRTPEALADAVHTLLGH"
     assembly_gap    6079..6178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6181..8127
                     /locus_tag="PLB86_08305"
     CDS             6181..8127
                     /locus_tag="PLB86_08305"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HRC65176.1"
                     /translation="MTSGIIRANLDLSIRPQDDLFGYTNGTWFAQTEIPADRGRYGTF
                     DHLREQAEHDLREILDEAVASGAAPDTSMGRVALLYGAFLDETAVQAAGLGAVAGLLA
                     EVDGVTNADDLVALLARLDRIGIGGAFHHYVNTDDRASDRYIGYLHQGGLGLPDESYY
                     REESYAETRAAYLRHVTAMLTLAGRPEPEAAAVRVLSLETRLAAGHLDKVADRDPVAT
                     YNLLTAAALADLAPHVDWAGYAAALGSPDALTEVVVRQPDYLRALSDALAEVSIGTWQ
                     DWLAWQILHSRAPFLPQEFVEENFDFYGRTLAGTPQLRERWKRGVELIDRTLGEALGE
                     LYVARHFPPAAKARMSELVSNLVEAFRRSFADRQWMGEDTKAQALAKLEQFTPKIGYP
                     ETWRDYSALVLTGDLLADVAAANAFESDRSLAKLGRPVDRGEWFMSPQTVNAYYNPGM
                     NEIVFPAAILQPPFFDVEADDAVNYGGIGGVIGHEVGHGFDDQGSQYDGSGNLRNWWT
                     DDDREAFDALGAKLVEQFNRLEPRAAPGRTVNGALTLGENLGDLAGLGVGHAAYRIAC
                     GDQQPPVLDGLTGDQRFFVGWAQVWCGKAREETAVMLLAVDPHSPAEHRANVVRNIDA
                     FHEAFGVGEGDGMWLDPADRVSVF"
     gene            8172..>8841
                     /locus_tag="PLB86_08310"
     CDS             8172..>8841
                     /locus_tag="PLB86_08310"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HRC65177.1"
                     /translation="MAIAWWARLGFQVEFEHRFEPHLPLYVGLRRDDAIVHLSEHAGD
                     ARAPGLVYVYVPDIDRIAAEFGVSVNEAPWAREVQLVDPDHNRVRCGCVPPASDAPQP
                     QLRVSTVNLSAADVPGLSRFYAQLLGYAVDEDNPDWHYLAASDGSVALSVQHEPTHLP
                     PVWPQSAEAQQMQAHLEIRVDGDLGAAVDRAIALGATLAAYQPQDDVRVLLDPAGHPF
                     CLWVP"
BASE COUNT         1281 a         3037 c         2990 g         1433 t
ORIGIN      
        1 gccgggttgc ccctgctcgt agccatcgtc tgcgtcattc ccctcgtcgt cggtgtcgcc
       61 gttcttctgg aacggatgac catcgcgccc gtcaagaaga tgaccccgct cacctcgatc
      121 atcctcaccc tgggcgtatc gacagcggtg aaggcggtca tgctgctcat ctgggggccc
      181 gaagccaagg gcctgccgaa cttccccggc tccgacatga cccttggcgg ggtgagcatc
      241 cgcagccagg agttgtgggt gctcggcatc ggggcgctcg tggcctatgc cgtcatctgg
      301 ttctacgagc acaccgtgcg gggtaagtcc ctgcgcgcgt gcgccgagca accgattgct
      361 gcccggctcg tcggcatctc gccccggacg gccaccgtcg tcgcgttcgc ggtcgccggc
      421 ttcaccgggg ctgtcgccgg catcgtcggc agcccgatct acctcagctc gtggcagagc
      481 ggcctgagcc tgggtctcaa gggcttcgtc gcagccaccc tcggcggtct gacgtcgctg
      541 cgggtcgcca tgatcggcgg catggccctc ggcgtcctgg agaacctcgt cgccggttac
      601 ctgtcctcgg gttaccgcga tgcggtggcc ttcgtcgtcc tcgtcctggt gctgctcttc
      661 cggcccaacg gtttggccgg caagacctcg gcggtccgcg tatgaacccg ctgttcaagc
      721 gccagatccc cttcatcgcc ctgttggtcg ggctgctcgt gttcggagcc gccgcgccgc
      781 aacagatggt cgatgtcggg atctgggcgc tcatctatgg tttcgcggcc atcgggctgt
      841 cgctgctcat gggtctggcc ggtcaggtca gcctcgggca cgccgccttc atcgccgtcg
      901 gtgcctatac gcaggcgttg ctcgtggcca aggccaacct caacccgctg ctcgcggcgc
      961 cgatctcggt cgtcgcagcc atgctcgtcg ccctgctcgt ggggttgccg ctcctgcggt
     1021 tgcgcggtca cttcctcgcg ctggccaccc tcggcctggg catcatcgtc ggggtgttcg
     1081 tcaccgagca ggagttcacc ggtggcacgt ccggcatctt tggtctgccc aacatctcgt
     1141 tcggcggccg ggtttacgac acatcccagg agtacttcct cctgctgacc cccgtcgtcg
     1201 tcatcgggct ctggctggcc agcaacctcg tgcacagccg caccgggcgc gcactcagcg
     1261 cggtcaacga ctccgaggtc gcggccgagt gcctcggcgt ggacacgttc gccctgcggc
     1321 ttcgcgtctt cgtcgtcagc gccgggtatg ccggtctggc cggtgtgttt tacacacact
     1381 ggttgaccat cgtcaacccg tcggtgtcgc acttcgagtt gtcggtgaag atcctgctga
     1441 tggtcgtgct cggcggcctc ggcaccgtgt ggggcgctct catcggtgct gtcgcggtgc
     1501 agttgctcga cgacgggctg cgtgggctca tccccatcct catcccgggg gccaagggcg
     1561 agatccagct catcggcttc ggcgtcgtcc tcgtgctcgt catcatcctc atgcccggtg
     1621 gactggctca actctgggcg cggctcatga gcgcggtccg ccagcgcaag caccccatcg
     1681 ttcgcgggga gcacgaaacc cccgtttcgc aggaggagca gcgggcgctc ctcgaggagt
     1741 cgctcaaact gctcgccaga gccgagcaca tgccggcggg gcagaccatc ctcgccatcc
     1801 gcggcctcac caagcgctac ggcggggtga cggcgctgga caacctcgac ctggacgtca
     1861 aggccggcga gatcatggcg ctcatcggtc ccaacggtgc cggcaagacg acggcgttca
     1921 acatgatcac cggcgtcatg gtgcccaccg agggttcggt gctgctgcaa ggcaccgagg
     1981 tcgccggaaa gcggccgcat atcgcggctg agatcggcgc cacccgcacc ttccaaaacc
     2041 tgcagacctt caagagcacg accgtgatcg gtaacgtcaa ggtcgcccgc cacctgcgca
     2101 gcaaggccgg gctcgtgcgc ggcatgctga tgttggaccg gaccgaggag cgcctcatcg
     2161 accaggcgtc ccaggcggcc atcgacgcga tggggctgac cagcctggca gacaagcaga
     2221 tcgccgacct ggccttcggc aagcaacgcc aggtcgaggt ggcccgcgcc ctcgcgctcg
     2281 agccgtcggt cctgctgttg gacgagccga tggccggcct gtccggtccg gagcgcgaca
     2341 gcctcagttg gctgctgcgt cgggtcaagg ccagcggcgt caccgtcctg ctcgtcgaac
     2401 acgacgtcgc ggcggtcatg gccctggccg accgagtggc cgtgctcgat gacggcaagc
     2461 tgatctcgct gggaactcct gcccacgtga ccagcgatcc gaaggtcatc gcggcatacc
     2521 ttggtgagga agacgaccag agcggcggtc ccgccacggg agcacccgcc gcgacatccg
     2581 gtgcggcagc gggaggcctg gcatgacgac cgaccacatt ccgcgcatcg ggctgcgggt
     2641 cgaggcgatc gacgcggcct acgaccggct ccaggtcctt tgggacgtca gcctgcacgt
     2701 cgagccgggg gagttgctcg ccctcgtcgg atccaacggc gcgggtaaga cgacggtcct
     2761 gcggtcggtc tcgggtctgc tcaagccggt ccggggtcgc gtgctgcttg gtgacaccga
     2821 catcacgtcg accagcgccg agaagatcgc cgagttgggg ctggctcacg tcccggagaa
     2881 ccgcctggtg ttcccgtcgc tcacggtcga ggacaacctc gtcctcggtg gctatgtccg
     2941 ccgacgcgac ggccggtatg ccgtccggcg cgacgaggcg ctgcatctgt tcccgcggtt
     3001 gcgcgaccgg atcaacctgc tggccggggc gctgtccggt ggcgagcagc agatgctcgc
     3061 gatggcgcgc gcattgatgt ctgacccgtc ggtcatcatc ctcgacgagc cgagcctggg
     3121 cctggcgccc aaggtcgtca ccgagatcat gagcgcgctc ggccacctgc gcgatgagaa
     3181 ggggctagcg gtgctgctcg tcgagcagaa cgtccgggcc gctttcaaga tcgccgaccg
     3241 ggtcctggtc atggaccgtg gccgggtcgt ggccgagggc acgcccgcgg agctggcctc
     3301 cgacgagcgg gtccgcgcgg catacctcgg cgggtcgtcg acctcatcga gcggcgtcgc
     3361 cgcccccgtc acccgctcag gcggcacgtc tgcctgagcc ctgtcccgca cgaactcgac
     3421 tagtcgctcg acgaaggaag tcctgccatg agcaagccgt tcgcctcttc caccgacctg
     3481 gccgctaaga cggagaccct cgaggttctc gccgacggcg tctacgccct caccgccgag
     3541 ggtgacccga acgtcggggc gatcgagggg gaggacttcc tcgtcgggtt cgaggcgcgg
     3601 gcgacccctg cggccgcgcg cgactggatc gccaagctgc gcgagcacac ggacaagccc
     3661 ttccgttacc tcgtgctctc gcactaccac gcggtgcggg tgctgggcgc ggaggcgttc
     3721 gaggccgacg agatcatcgc ccacgagcgc acccggttcc tcgtccagga gcgcggcatg
     3781 caggactggg cgagtgaata cggccggatg ccaaggctgt tcaaggagcc cgagggcatc
     3841 aagggtctca cctggccgac gctgaccttc tcggacgaga tgaccatccc gctgggtggc
     3901 gaccgcggtg acctggtcct gaagtacctc gggcgaggtc acaccgaggg cgacatcgtg
     3961 gcctggctgc ccaagcacaa gatcatgttc gccggcgacc tcgtcgaggc caaggccgcg
     4021 ctctacaccg gcgatgcgtt ccaccaggag tgggcctcga ccacgctcga cgcggtcaag
     4081 gcgatgggtg ccgaggccat cgtcggtggg cgcggcgaaa tcccgcgcgg cgaggcggcc
     4141 gtcaacgcgg ccatcgagca gtcccgtggc ttcatcacgt cgatcatcga caacgtccgg
     4201 gccacgcagg ccgcgggggg caccctcaag caggccttcg acgcgtgcca cgccgcgctg
     4261 tcgccgacct acggtcactg gccgatcttc gagcacaccc tgcccttcga cgtggcccgc
     4321 acgtgggaag agctgcaggg cgtcgagcgg ccgacgatct ggaccgccga gcgcgatcag
     4381 gagatttggg cccaactgca gggctagcgt gtgggctagc ctccgacgat cggttactcc
     4441 actcccggca ggagcgacat gtcgcaggat cacggcaccg aggggcgcgg gcgcgtcctc
     4501 gtcatcggta acggcccggt cggtcagacc gcggccctgt gtctggcccg atgggggatc
     4561 cccgtcctcg tgctggactc acgcccggag cgggacccca tcgggagcaa ggcgttgtgt
     4621 cagcagcgcg atgtcctgga tctgtgggac gcctgcgggg cgggtgctgt cgcgaccgaa
     4681 gggctgacct ggtcgcgggc gcgcaccttc tacaaggaca aggaactctt ctgctgggag
     4741 atccccgacc cggggcgctc cccgctgccg cccttcgtca acatctccca ggcccggtcc
     4801 gaggcgatcc tcgacgaggc gatcgctgcc caaccgctca tcgaggtgtg gtgggggacc
     4861 gaggtcgtgg gggtcgctgg gggcaagagc gacagtgacg gcttggcaac ggtggtgtgc
     4921 cgcagcggtt tcggtgagtt cgaggtcgcg ggggagtatg ccgtcgtctg cgccggatcc
     4981 aagggtggcc ccttgcggga ggcgctcggg gtgaccttcc cggggcgcac cttcgacgac
     5041 tcgttcctca tctgcgacat cgagaccacc ttgaaggggt gggagaacga gcgccggttc
     5101 tacttcgacc ccgagtggaa cccggggcgt caggtgctca tccacccgtg cccgaacagc
     5161 atcttccgga tcgactggca ggtcgacacc gacttcgacc tggcggccgc gcaggccgac
     5221 gggtcgctgg acaagcgcat ccgcaaggtc atcggcaaga agcgtgacta tcggatcgac
     5281 tgggtgtcgg tctatcgctt ccatgcgcgc agcgccgatc gcttccgggt tggacggttc
     5341 ctcctcgcgg gcgacgtcgc ccatctggtg gcgccgttcg gggcgcgggg cctcaacagc
     5401 ggcgtgccgg atgtcgagaa cgccgcctgg aagatcgcct tcgccatgaa ggggtgggct
     5461 ggtcctgggc tcgtcggcag ctacgagagc gagcgacagg ccgctgctcg cgagaacctc
     5521 gcggtgacgg ccgcgacgat ggacttcctt gtcccgcaga cgaagaaggc caagcatcgc
     5581 cgcaaggaga tcctcaagcg ggctcgcaag gacaagaagg tcatcccgct ggtcgactcc
     5641 ggccgactgg cggaggcctt ctggtacgtc gacagcccct tgaccacgcc cgatccgagc
     5701 cgtttctggc cgggtcgccc gcctcgcggg caggcgccga cccccgtgcc gggcgtcatc
     5761 ctccccgatg ccgtgacggc ttccgccggg catggcaaga gccgggtgcg cgagttggcc
     5821 cgggaggggc tgctcatcct cgtgggcgag gcagtcaaca cccacgaggt cgaggggctg
     5881 ttgtctgggg tcgtgccggc cggtctgccg gtgcgggtgc tgcacttctc ggacctcggt
     5941 gacgaggacg tcgtggccac cggcttgcag gcggtggccg aggagttgtg gctgatccgg
     6001 cccgacgcgc acaccgcggc gtgcgtccgc acccccgaag ctctggccga cgccgtccac
     6061 accctcctcg gccactgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     6181 atgacgtccg gaatcattcg cgcgaacctc gacctgtcga tccgacccca ggacgacctg
     6241 ttcggctaca ccaacggcac gtggttcgca cagaccgaga tccccgccga tcgaggtcgc
     6301 tacggcacct tcgaccacct gcgcgagcag gccgagcatg acctgcgcga gatcctcgac
     6361 gaggccgtgg cgtcgggcgc cgcacccgac acgtcgatgg gccgggtcgc cctgctctat
     6421 ggcgcgttcc tcgatgagac ggccgtgcag gccgccgggc tcggagccgt ggcgggcctg
     6481 ctggctgaag tcgatggggt gaccaacgcc gacgacctcg tggccctgct cgcccggctc
     6541 gaccggatcg gcatcggcgg tgccttccac cactacgtca acaccgatga ccgcgcctcg
     6601 gatcgctaca tcggctacct ccaccagggc ggtctcggcc tgcccgacga gtcctactac
     6661 cgcgaggaga gctacgccga gacgcgggcg gcatacctgc gccatgtgac cgcgatgctg
     6721 accctcgccg ggcgcccgga gcccgaggcg gccgcggtgc gcgtcctgag cctggagacc
     6781 cggctggccg cgggtcatct cgacaaggtc gccgaccgcg acccggtcgc cacctacaac
     6841 ctgctcaccg ccgccgcgct cgccgacctc gcgccgcacg tcgattgggc ggggtatgcc
     6901 gctgcgctgg gttcgcccga cgccctcacc gaagtggtcg tgcgccagcc ggactacctg
     6961 cgtgccctgt ccgacgcgct ggccgaggtg tcgatcggga cgtggcagga ctggctggcc
     7021 tggcagatcc tccactctcg ggcgccgttc ctcccgcagg agttcgtcga ggagaacttc
     7081 gacttctacg gccgcaccct cgccggcacg ccgcaactgc gcgagcgctg gaagcgcggt
     7141 gttgagttga tcgaccgcac gctgggcgag gccctgggcg agctctatgt cgcccggcat
     7201 ttcccgccgg ctgccaaggc ccggatgagc gagctcgtca gcaacctcgt cgaggcgttc
     7261 cggcgctcgt tcgccgaccg gcagtggatg ggcgaggaca ccaaggccca ggccctcgcc
     7321 aaactcgagc agttcacgcc gaagatcggc taccccgaga cgtggcgcga ttacagcgcc
     7381 ctcgtgctca ccggcgacct gctggctgac gtggccgcgg cgaacgcctt cgagagcgac
     7441 cgcagcctcg ccaagcttgg ccggcccgtc gatcgtggcg agtggttcat gagcccgcag
     7501 acggtcaacg cgtactacaa cccgggcatg aacgagatcg tcttccccgc cgcgatcctg
     7561 cagcccccct tcttcgacgt cgaggccgac gacgcggtga actacggcgg catcggcgga
     7621 gtcatcgggc acgaggtcgg gcacgggttc gacgaccagg gcagccaata cgacggctcg
     7681 ggcaacctgc gcaactggtg gaccgacgat gatcgggagg ccttcgacgc cctcggtgcc
     7741 aagctcgtcg agcagttcaa ccggctcgag ccccgcgccg ctccgggacg gaccgtcaac
     7801 ggagcactca ctctgggcga gaacctcggt gacctggccg ggctgggagt ggggcacgcg
     7861 gcataccgga tcgcctgcgg tgaccagcag ccgcctgtgc ttgacgggct gaccggcgac
     7921 cagcgcttct tcgtcggctg ggcccaggtg tggtgcggca aggcgcgcga ggagaccgcc
     7981 gtcatgctcc tcgcggtcga cccgcactcg ccggccgagc accgcgccaa cgtcgtgcgc
     8041 aacatcgacg ccttccacga ggcgttcggc gtgggggagg gcgacggcat gtggctcgac
     8101 cccgccgacc gggtgtccgt cttctgatgc gggccaacgc ggctcccgtt ctgcgggtcc
     8161 gagatgcccg ggtggcgatc gcgtggtggg cccggctggg gttccaggtc gagttcgagc
     8221 accgtttcga gccgcacttg ccgctctatg tcgggctgcg acgcgacgac gcgatcgtcc
     8281 acctctctga acacgcgggc gacgcccgcg cgccgggcct cgtctatgtc tacgtgcccg
     8341 acatcgatcg gatcgcggcg gagttcgggg tgagcgtgaa cgaggcgccg tgggcacgcg
     8401 aggtgcaact ggtcgaccct gaccacaatc gggtgcgctg cgggtgcgtc ccgccagcct
     8461 cggatgcgcc gcaaccacag ttgcgcgtct ccacggtcaa cctctcggcc gccgatgtcc
     8521 cgggtctctc gcgcttctac gcccaactgc tcgggtatgc cgtggacgag gacaatcccg
     8581 actggcacta tctggccgcc tcggacggct cggtggcgct gtccgtgcaa cacgagccca
     8641 cccatttgcc gccggtctgg ccgcagtccg ccgaagccca acagatgcag gcgcatctgg
     8701 agatccgggt agatggcgac ctgggagcgg ctgtcgaccg ggcgatcgcg ctgggagcca
     8761 cgctcgcggc ataccagccg caggacgatg tgcgcgtgct gctcgacccg gcgggacatc
     8821 cgttctgcct atgggtgccg a
//
LOCUS       DAORPW010000090         8821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1909_length_8821_cov_694.444444, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000090 DAORPW010000000
VERSION     DAORPW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8821
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1909_length_8821_cov_694.444444"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..946
                     /locus_tag="PLB86_08315"
     CDS             89..946
                     /locus_tag="PLB86_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65178.1"
                     /translation="MDSDHSIAVRNTYRYLRLAMIAVVLMLFLAVGIERLASTGWQTS
                     ISAYYFTAVHAVFIAALCTIGACLIVYQGNTDTEEVVLNFSGFLAFVVAFVPTQREPL
                     YGPGLPATYEVGMGIRNNVLALIITGVVVEIARIIINRNVDRRPLSPWAKRATLIGWA
                     VIGVGILGYAAFPANFEAKGHTVAAVTMFVGIIAVIVLNALSAQSAQTGPSYVGSYKL
                     IAGLMALLVLAIVVIRLTLPEFAHIVIWIEGVLILAFAAFWLVQTKELWEVVDRRDLA
                     PNTTDPTGH"
     gene            1034..2212
                     /locus_tag="PLB86_08320"
     CDS             1034..2212
                     /locus_tag="PLB86_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018763778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HRC65179.1"
                     /translation="MNQPGPSSAAAAPTASPAERAAYAAASVTTLFGRRMLGVPGTFL
                     PLVTSPGPRRWRGPWHYWWLAHYLDCLVDQALREHAAGDLAGARATTATARRLLRTIR
                     IRNVAIFTNHYYDDMAWLLLAVHRLDRLTARLSPGTSSALTHSAGRALRAAVTRGHTI
                     DLGGGLFWNDHHDFKNVAATGPAALFFARIGDRARARSLLDWLHDHLRDPATGLFVDG
                     LRIAPDGTTTLVPDVFTYNQGTVLAALITLGDPISLARAEALVSAVADHLTTDPDHRV
                     LRTHGDGDGGLFTGILARYLALAAHSPALADGARQVARELVRGTAEAFWSGRQLRSAP
                     TTSGMPSAPATALPSVAVFSPDPTVSAEQTQPRGIPLELSTQLQAWMTLEAAATLATA
                     "
     assembly_gap    2297..2396
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2397..2609
                     /locus_tag="PLB86_08325"
     CDS             <2397..2609
                     /locus_tag="PLB86_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65180.1"
                     /translation="FTLPGPFTRNRWVVAVLYLAVLVLGAFPTVAEPLGAAPIQVAAV
                     LLIGLVVLGHGLTWEFMTDSSDAVAD"
     gene            2734..4128
                     /locus_tag="PLB86_08330"
     CDS             2734..4128
                     /locus_tag="PLB86_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC65181.1"
                     /translation="MSTAGGAAATRAPKSAETREQKSWYWYDWANSAYVTTTATVLFA
                     PYLTSVATAAACPDLADGQRCAATLSVLGIPVSPGSLVAYTATVSTIISAIFLIFVGA
                     IADRSPHPTKLFATFAWTGALSATLMCLVTGTNWQMGVLLFVIANICLGSSLVIYDSL
                     LVRIAGPNDRDRVSSKGWAFGYLGGGLLLLLNFVLVAKPDLVGLDKGGAVRVSLLMAG
                     VWWAAFTIIPVRGLRNVTGTVAPPVAPGEGVVGGSLRQLRETFRDLALHYPQTKLFLL
                     AYLFFNDGIQTVIGNSSLYGIEELEFPQETVLGIFLFVQFVAFFGARFFGQVAARIGA
                     WKTVLSSLGVWTGIVTVAFFVPAKSLVLFLALAFFIGMVLGGSQALSRSLYSQLIPAG
                     KEAEYFSLYQAMERGTSWFGTLIFGLVYQATLSYRAAILALIVFFILGGVLLSRVKMR
                     EGIIQAGNEVPRVI"
     gene            4268..4624
                     /locus_tag="PLB86_08335"
     CDS             4268..4624
                     /locus_tag="PLB86_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HRC65182.1"
                     /translation="MADRSLRGTRLGALSMESDVNVVPSARQITVYVCPQGHRSELPF
                     SIEADIPPTWECRCGASARLLNGESVPDAKPARPPRTHWDMLLERRSIPELEELLAER
                     LALLRASRGETSKRSA"
     gene            complement(4724..5218)
                     /locus_tag="PLB86_08340"
     CDS             complement(4724..5218)
                     /locus_tag="PLB86_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HRC65183.1"
                     /translation="MAQRVTRSGGRRRWPWVLTLALLVVPTAELAIILWVGQQIGGWP
                     TLGLLVAESALGAWLVKREGSRTWQALASALNTGRMPSRQLSDAALVLVGGTLLLTPG
                     FLTDLVGFFCILPITRAIARTVLETIVTRKLLGGIVSSGDRPPRSPGGSGPGDVIEGE
                     IIDG"
     gene            complement(5203..6000)
                     /locus_tag="PLB86_08345"
     CDS             complement(5203..6000)
                     /locus_tag="PLB86_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRC65184.1"
                     /translation="MTESSTRGALRRIVVLIPTYNERENLPRIVGRVRAAVPAVDILV
                     LDDNSPDGTGAVADDLAAADAAVHVLHRAGKQGLGAAYKAGFAWAIERGYDAAVEMDA
                     DGSHQPEQLPSLLRAAENADLVIGARWIKGGSVVNWPIHRKVLSVGANIYTKVMLGLP
                     VNDATGGYRVYRTSALQTMDLGSVSSAGYGFQVDMTVALVRAGLVAVEVPIEFVEREI
                     GESKMSGNIVGEAFVNVGKQGLAHRAGQLRGAAARLRSPREGRWHSA"
     gene            complement(5997..>6320)
                     /locus_tag="PLB86_08350"
     CDS             complement(5997..>6320)
                     /locus_tag="PLB86_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRC65185.1"
                     /translation="TNNATFGFTNESVQQLAISRIRAIEHGRSVVHVSTVGVSALILP
                     DGTLLQPTSLFTPAALAADLPLRTERTLAGWVGAWPEYCAVFGMLALLAGSALSRRQR
                     KELTS"
     assembly_gap    6321..6420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6421..6695)
                     /locus_tag="PLB86_08355"
     CDS             complement(<6421..6695)
                     /locus_tag="PLB86_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRC65186.1"
                     /translation="MRRLLLALVGGAVLSLAFPGYGLWWAAPPAIAILALVTVGVRGR
                     AGFGLGLAFGLAFFVPTLSWSGIYLGVVPWAGLAVCQALYVGLLGWG"
     gene            complement(7195..7590)
                     /locus_tag="PLB86_08360"
     CDS             complement(7195..7590)
                     /locus_tag="PLB86_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRC65187.1"
                     /translation="MSTNPSRAEVGRTVVHRRYVPYSHAHYAGNLVDGAYSLAAFGDV
                     ATEMCILTDGDEGLFASYSDVQFLAPVRAGDVLEIEARLVRVGRRSREMDFAVRVVCR
                     GTPSEGASAAEVLDPPVVATTARGTVVVP"
     gene            complement(7587..8384)
                     /locus_tag="PLB86_08365"
     CDS             complement(7587..8384)
                     /locus_tag="PLB86_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HRC65188.1"
                     /translation="MTNRKHLRETPRKVTPQIVRPYGDTTGDGMVQMSFTLPLPHDKR
                     AEGAALQLAKKMGMDPALVVHAKPIGPDFTFFVVYGSSNHLVDLSQVHVQEREFPLLT
                     PKEVNVAVKRGLRRPLVVVGACIGTDAHTVGIDAILNIKGFAGEKGLEYYSEITVVNL
                     GAQVLVPELVAKARAAHADAVLVSQVVTQKDAHIHNVSQMTAAFREAYPAGKVPLLVA
                     GGPRFEDGSAQRLGVDRIFGRGTTPGEVASYLVHAIVPPATPRKKAS"
     gene            complement(8381..>8821)
                     /locus_tag="PLB86_08370"
     CDS             complement(8381..>8821)
                     /locus_tag="PLB86_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HRC65189.1"
                     /translation="PTKHMTGDVFRGYLLDGFFNLAGTMTGQGILLVGMMTEAVVTPF
                     LSDRDLALQNVRYVLDAAGGLREDFHPPRDGFIQTRSRHVLGEAVDLLDRIATDGLLD
                     AIADGTFGLMKRPADHGKGLDGVIVRAPDYDNPAVTLLETGATR"
BASE COUNT         1334 a         3082 c         2827 g         1378 t
ORIGIN      
        1 gaataccgcg ccgcgacagc cgcgttgcgg gacactcagc gggtacctgc cctgcctctc
       61 ccccgcagca cccccggaag ggctcacgat ggactctgat cacagcatcg ccgtccgaaa
      121 cacctaccgc tacctgcgac tagccatgat cgccgttgtg ctcatgctct tcctcgccgt
      181 cggcattgaa cggctggcgt ccaccggctg gcagacctcg atcagcgcct attacttcac
      241 cgcggtccat gccgtcttca tcgcggcgct gtgcacgatc ggcgcctgcc tcatcgtcta
      301 ccagggcaac accgacacgg aggaggtcgt gctcaacttc tccggcttcc tcgcgttcgt
      361 cgtggctttc gtccccaccc agcgcgagcc gctctacggc cccggcctac ctgcgacata
      421 cgaagtcggc atggggatcc gcaacaacgt cctggcgctc atcatcacgg gcgtcgtcgt
      481 cgagatcgcc cgcatcatca tcaaccggaa cgtcgatcgc cggccgctca gcccctgggc
      541 caagcgagcg accctcatcg ggtgggcggt catcggcgtc ggcatcctcg gctacgccgc
      601 cttccccgcc aacttcgagg ccaagggcca cacggtggct gcagtgacga tgttcgtcgg
      661 catcatcgcc gtcatcgtcc tcaatgccct gtcggcgcag agcgcacaga ccggcccgag
      721 ctacgtcggc agctacaagc tcatcgccgg gctcatggcg ctcctcgtgc tggccatcgt
      781 cgtcatccgc ctcaccctgc ccgagttcgc ccacatcgtc atctggatcg aaggcgtcct
      841 catcctcgcc ttcgcggcct tctggctggt gcagaccaag gagctgtggg aagtcgtcga
      901 ccgtcgcgac ctcgccccaa acaccacgga ccccaccggc cactgaaccg tccgccacgg
      961 ggagccggca cgacccgctc ggtatgccgc gtgccccaca cgccgccatc gtggcccctg
     1021 gcaggatgac cgcgtgaacc agccgggacc ctccagcgcc gcagcggcgc caacggcatc
     1081 cccggccgag cgggcggcat acgcggcggc ctcggtgacc accctcttcg ggcgacggat
     1141 gctcggggtc cccggcacgt ttctccccct cgtgacctcc cccggcccca gaaggtggcg
     1201 cggcccgtgg cactactggt ggctggcgca ctacctcgac tgcctcgtcg accaggccct
     1261 ccgggagcac gcggccggtg atctcgccgg tgcccgggcc accacggcca ccgcgcggcg
     1321 actgctgcgc acgatccgca tccgcaacgt cgcgatcttc accaaccact actacgacga
     1381 catggcctgg ctgctgctgg ccgtgcatcg cctcgaccga ctcacggccc ggctctcccc
     1441 cggcacctcg tccgcgctga cgcacagtgc cggccgcgcc ctgcgagccg cggtcacccg
     1501 gggccacacc atcgacctgg gcggcggcct gttctggaac gatcaccacg acttcaagaa
     1561 cgttgccgcg acgggcccgg cggcgctctt cttcgcccgg atcggtgatc gcgcgcgggc
     1621 gcggtccctg ctcgactggc tgcacgacca cctgcgtgac ccggcgacag gcctgttcgt
     1681 cgacggccta cgcatcgccc cggacggcac caccaccctc gtgcccgacg tgttcaccta
     1741 caaccagggc accgtcctgg ccgcgctcat cacgctcgga gaccccatca gcctcgcgcg
     1801 cgccgaggca ctggtgagcg cggtcgccga ccacctgacg accgaccccg accatcgggt
     1861 cttgcgcacg cacggcgacg gtgacggtgg gttgttcacc ggcatcctgg cccgctacct
     1921 cgctctcgcg gcccactccc ccgccctcgc cgacggtgcc cggcaggtgg ctcgcgagct
     1981 cgtgcgcggc accgccgagg cgttctggtc aggtcgccaa ttacgctccg cgccaacgac
     2041 atccggtatg ccgtccgccc ccgcgacggc gctcccctcg gtcgcggtgt tctcccccga
     2101 cccaacggtg tcggccgagc agacccagcc gcgcggcata cctctggagt tgtcgaccca
     2161 actgcaggcc tggatgaccc tcgaagcggc tgcgaccctg gcgactgcgt gatggatcgg
     2221 ccgcgccggc ccggtctgcg gcgctacctt ggcccatgga catcggcacc ttctactcgt
     2281 tgttctcggc cacgtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttca
     2401 ccctccccgg cccgttcacg cgcaaccggt gggtggtggc cgtgctctat ctcgccgtcc
     2461 tggtcctggg cgccttcccc accgtggccg aaccgctggg tgccgctccg atccaggtgg
     2521 ccgcggtcct gctcatcggc ctggtcgtcc tcggccacgg cctgacgtgg gagttcatga
     2581 ccgattcttc tgacgcggtc gccgactgaa gggcgcgcgt cgtcaccggg atgcccggga
     2641 tgacggagat gcccgagatg ctcgccgggg cgctcggcgt ggcgcacggc atacccctgc
     2701 cgcgatcccg gcgggtgtga cacgcttcac gacatgagca ccgccggggg ggccgccgct
     2761 acgcgcgccc caaagtcagc agagacccgt gagcagaagt cctggtactg gtacgactgg
     2821 gcgaactcgg cctacgtcac gacgacggcg accgtgctct ttgctccgta tctcacctcg
     2881 gtcgcgacgg ccgctgcctg ccccgacctc gccgacgggc agcgatgcgc cgcaacgctg
     2941 tcagtgttgg ggatccccgt ctcccccggg tcgctcgtgg cctacaccgc aaccgtctcg
     3001 acgatcatct cggccatctt cctcatcttc gtcggagcga tcgcggaccg ctccccccac
     3061 cccacgaaac tgttcgcgac ctttgcctgg accggcgccc tctccgcgac cctcatgtgt
     3121 ctggtcacgg gcaccaactg gcagatgggt gtgctgctgt tcgtcatcgc caacatctgt
     3181 ctgggctcgt cgctggtcat ctacgactcc ctcctcgtgc gcatcgccgg tccgaacgac
     3241 cgcgaccggg tgtcctcgaa aggctgggcc ttcggctatc tcggcggagg gttgctgctg
     3301 ctgctcaact tcgtcctcgt cgccaaacct gatctggtcg gactggacaa gggtggcgcg
     3361 gtccgggtga gcctgttgat ggccggggtg tggtgggccg ccttcaccat catccccgtg
     3421 cgcgggctgc gcaacgtcac cggcacggtt gccccaccag tggccccggg cgagggtgtc
     3481 gtcggaggca gcttgcggca gttgcgtgag accttccggg acctggcgct gcactatccc
     3541 cagacgaagc tgttcctgct ggcctatctg ttcttcaacg acggcatcca gaccgtcatc
     3601 ggcaactcca gcctctatgg gatcgaggag cttgagttcc cgcaggagac agtgctgggg
     3661 atcttcctct tcgtccagtt cgtcgccttc ttcggtgccc gcttcttcgg gcaggttgcc
     3721 gccaggatcg gtgcctggaa gaccgtgctg agcagcctgg gcgtctggac gggcatcgtc
     3781 acggtcgcct tcttcgtccc cgccaagtcg cttgtgctct tcctggcgct ggcgttcttc
     3841 atcggcatgg tgctcggcgg atcacaggcc ctgtcgcgct cgctctacag ccagctgatc
     3901 ccggcaggca aggaggccga gtacttcagc ctttaccagg cgatggagcg cggcacgagc
     3961 tggttcggca cgctgatctt cgggttggtc taccaagcga ccctgtccta ccgcgccgcc
     4021 atcctcgcgc taatcgtctt cttcatcctc ggcggcgtgt tgctgtcccg cgtgaagatg
     4081 cgcgaaggca tcatccaggc tggcaacgag gtacctcgcg tcatctgacg ggcgttatgt
     4141 cgggtgaatg ctgacgactt cgggaacaaa ggcaaccctt tgcgccgttg gaacgtctga
     4201 gttggtgagc agggggctgc ggcatccgac cggacccctc cccggctcgc ccgtcaggag
     4261 gttcagcatg gccgaccgtt ccctccgagg cacccgcctc ggcgcactga gcatggagtc
     4321 cgacgtcaac gtcgttccga gcgcacgaca gatcacggtc tacgtctgcc cccaggggca
     4381 ccgctccgag ttgccgttca gcattgaggc cgacatcccc ccgacctggg agtgccgttg
     4441 cggcgccagc gcgcgcctgc tcaacggcga gtcggtgccg gacgccaagc cggcgcgacc
     4501 cccgcggacc cactgggaca tgttgctcga acgtcgatcc atccccgagc tcgaggagct
     4561 gctcgccgag cgccttgccc tgctgcgcgc ctcgcgcgga gagaccagca agcgcagcgc
     4621 ctgagtccgc acccggcata ccaaggaacc cgcaccttca ccccggaagg tgcgggttct
     4681 gcctgtccct ccccaaacaa ggctgagcaa ccgccaagcg ggctcaaccg tcgatgatct
     4741 cgccctcgat gacgtcgccc ggcccgctcc cgccaggtga ccgagggggc cggtctcccg
     4801 agctgacgat ccccccgagg agtttgcggg tgacgatcgt ctcgagcacg gtgcgagcga
     4861 tcgcccgggt gatcggcagg atgcagaaga accccacgag gtcggtgagg aagcccgggg
     4921 tgagcagcaa cgtgccgccc accaacacca gcgccgcgtc actgagctgg cgtgagggca
     4981 tccggccggt gttgagggcc gacgcgaggg cctgccaggt gcgggaccct tcacgtttga
     5041 ccagccacgc gcccaacgcc gactcggcca cgagcaggcc cagcgttggc cagccaccga
     5101 tctgctgacc gacccagagg atgatggcca actcggccgt gggcacgacg agcagggcca
     5161 gcgtgagcac ccaaggccag cggcgacggc cgccggagcg ggtcacgcgc tgtgccatcg
     5221 accctcccgc ggactgcgca gccgggcggc cgcgccgcgc agttgcccgg cccgatgtgc
     5281 caggccctgc ttgccgacgt tgacgaacgc ctcgccgacg atgttcccgc tcatcttcga
     5341 ctcaccgatt tcgcgctcga cgaactcgat gggaacctcg acggccacca ggccggcccg
     5401 caccagggcc acagtcatgt cgacctggaa gccatagccc gccgagctga ccgaacccag
     5461 gtccatggtc tgcagcgccg acgtgcgata gacccggtaa cccccggtcg cgtcgttgac
     5521 cggcagcccg agcatgacct tggtgtagat gttcgccccg acgctgagga ccttgcggtg
     5581 gatcggccaa ttgacgaccg agccgccctt gatccaccga gccccgatga caaggtcggc
     5641 gttttccgcc gcccgaagca ggctcggcag ttgctccggt tggtgggagc cgtcggcgtc
     5701 catctcgacg gccgcgtcgt agccccgctc gatcgcccac gcgaagcccg ccttgtatgc
     5761 cgcgccgaga ccctgcttgc ccgcgcggtg caggacgtgc accgcagcgt cggccgccgc
     5821 caggtcatcg gcgacggcac cggtgccgtc gggcgagttg tcgtccagga cgagaatgtc
     5881 cacggcgggc acggcggcgc gcacccggcc gacgatgcgt ggcaggttct cccgctcgtt
     5941 gtaggtggga atgagcacga cgatgcgtcg cagggctccc cgggtcgacg actcggtcat
     6001 gaggtcagtt cctttcgctg ccgcctgctc aaggcggagc ccgcgagcag ggcgagcatc
     6061 ccgaacactg cacaatattc cggccaggcc cccacccaac cggcgagggt ccgctcggtg
     6121 cgcaggggaa ggtcggcagc cagggcagcc ggtgtgaaca acgacgtcgg ctgcaggagt
     6181 gttccgtcag gaaggatgag cgcgctcaca ccaacggtgg acacgtggac gaccgagcgg
     6241 ccgtgctcga tggcccggat ccgtgagatc gccagttgct ggaccgattc gttggtgaac
     6301 ccgaaggtcg cgttgttggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 ctcccccagc caagcagacc cacatacagg gcctgacaca ccgcgagccc ggcccacggc
     6481 acgaccccga ggtagatgcc cgaccaactc aacgtcggga cgaagaaggc cagcccgaag
     6541 gccagcccga ggccgaagcc agcgcggccg cgcactccga cggtgaccaa cgcgaggatc
     6601 gcgatcgcgg gcggcgcggc ccaccacagc ccgtaaccgg ggaaggccag ggacagcacg
     6661 gcaccaccga cgagagcaag gaggaggcgg cgcacaggtg caaccgtagg tcagcgcggc
     6721 gagaactccg aacgcccctg gtggtccgcc gacgtcgggt gcggacgtcg ctgtgcccgt
     6781 gctgctgttg ggaccaaccg ccacccctcc ggatgaggga tgcccggttg ttgtctacta
     6841 ggcatgcacg ggcacaaccg acgtccgcca cacagttacg acaggcgatc ccgccccgtg
     6901 gcagcgagcc tggtcgcccg gtggccgcgc gcgcaggcca ccgtggactc ccaccccggg
     6961 acgttgccgc ttttcgtagc agagccaccc aaaggcgaac aacgtgaccg aacttacccg
     7021 ctgtgtctcg gctgtcaact cgaccctgac ctgcacaaat gcgcgaaagc gcaggtcagg
     7081 aggcgtccgt gacgggtgtt ctcggaaagg gaaatccgac gtacacggcg tgtcgtgaac
     7141 gacacgccgg ggtgccttcg gagtgacctg cgagtgaccg gcgggtgtcc agactcaggg
     7201 gacgacaacc gtcccacgag cagtcgtggc gaccaccggc gggtcgagga cctcggcggc
     7261 cgaggccccc tccgaggggg tgccgcggca taccactcgg acggcgaagt ccatctcacg
     7321 ggaccggcgc ccgacccgca cgaggcgggc ttcgatctcg aggacgtcac ccgcgcgcac
     7381 cggcgcgagg aactggacgt cggagtagga ggcgaacagc ccctcgtcgc cgtcggtgag
     7441 gatgcacatc tcggtcgcca cgtctccgaa ggcagccaac gagtaggcgc catcgacgag
     7501 gttgccggcg tagtgggcgt ggctgtaggg gacgtagcgg cggtggacga cggtgcggcc
     7561 gacctcggcg cgggacgggt tggtgctcat gaggccttct tccggggcgt cgcgggcgga
     7621 acgatcgcgt ggacgagata gctggcgacc tcgccgggtg tggtgccccg gccgaagatg
     7681 cgatcgacgc ctaggcgctg cgccgacccg tcctcgaagc gcggtccccc ggcaaccagc
     7741 agcgggacct tccccgccgg gtaggcctcc cggaaggcgg ccgtcatctg cgagacgttg
     7801 tggatgtgag cgtccttctg ggtgacgacc tggctgacca ggacggcgtc ggcgtgggcg
     7861 gcacgggcct tggcgacgag ttcgggcacg aggacctggg cgccgagatt gacgacggtg
     7921 atctcggagt agtactccag acccttctcc cccgcgaacc ccttgatgtt gaggatggcg
     7981 tcgatcccca ccgtgtgggc gtcggtgccg atgcaggccc cgacgacgac gaggggtcgg
     8041 cgcaggccgc gcttgacagc gacgttgacc tccttggggg tcaacagtgg gaactcgcgc
     8101 tcctgcacgt gcacctggga caggtcgacg aggtggttgg acgagccgta gacgacgaag
     8161 aaggtgaagt cggggccgat cggcttggcg tgcacgacca gggccgggtc catgcccatc
     8221 ttcttggcca gttggagggc ggcaccctcg gctcgcttgt cgtgcggcag cggcagggtg
     8281 aaggacatct ggaccatccc gtccccagtc gtgtcgccat aggggcggac gatctgcggg
     8341 gtgaccttgc ggggcgtctc gcgcagatgc ttgcgattgg tcatcgcgtg gctcccgtct
     8401 caagcagggt gaccgctggg ttgtcgtagt caggcgcgcg gacgatcacg ccgtccaggc
     8461 ccttgccgtg gtcagccggc cgcttcatca gcccgaaggt gccgtcggcg atggcgtcga
     8521 gcaacccgtc ggtggcgatc cggtcgagca ggtcgacggc ctcgccgagg acgtggcgcg
     8581 aacgggtctg gatgaagccg tctcgcggcg ggtggaagtc ttcgcgcagc cccccggcgg
     8641 cgtccaggac gtagcggacg ttctgcaacg ccaggtcacg gtcggacagg aacggggtga
     8701 ccacggcctc ggtcatcatc ccgacgagca ggatgccctg gccggtcatg gtgccggcaa
     8761 ggttgaagaa cccgtcgagc aggtagccgc ggaacacgtc gccggtcatg tgcttggtcg
     8821 g
//
LOCUS       DAORPW010000091         8807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1917_length_8807_cov_718.924931, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000091 DAORPW010000000
VERSION     DAORPW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1917_length_8807_cov_718.924931"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="PLB86_08375"
     CDS             <1..1280
                     /locus_tag="PLB86_08375"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRC65190.1"
                     /translation="RNTGKLCFATLQEGAGTRLQVMLSLAEVGEAALADWKALVDLGD
                     HVAVEGRVVSSKRGELSVMATSWQMASKALRPMPTLHKDLSEETRVRQRYADLVVRQE
                     ARDMVRLRARATRAIREVLDTQGYLEIETPVLQLVHGGAAARPFRTHLNAFDQQMTLR
                     IALELNLKKAVVGGVDRVYEMGRIFRNEGVDGTHSPEFTMLEAYQAYGDQTTIAALIR
                     DLYLGVADALGTRVVQTPRGEVDLGGEWRWLPVYDAVSDAVGEEVTLATPVETLRRYA
                     AAHDVPLDPAWGADKVVLELLSELVEPKVLQPTFFCDYPAIAQPLARPHRSVPGLIEA
                     WDLIIAGVERGTGFSELIDPVIQRQVLEAQSLLAAGGDPEAMALDEDFLRALEFGAPP
                     MGGLGLGIDRLVMLFTGASIRETILFPLLKPED"
     gene            1283..1474
                     /locus_tag="PLB86_08380"
     CDS             1283..1474
                     /locus_tag="PLB86_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
                     /protein_id="HRC65191.1"
                     /translation="MWDAIYPYLAAILPTIGLLVIAYVVFKTIFEADRRERKAVAEWE
                     RSHDASPISSTDDESRPAT"
     gene            1835..2164
                     /locus_tag="PLB86_08385"
     CDS             1835..2164
                     /locus_tag="PLB86_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HRC65192.1"
                     /translation="MAKRVQVVLEDDVDGSPADETVSFGLDGVSYEIDLSAANASALR
                     DQLAKWIGAAQRTGGRRAAGRRAGGSARRTDVSAVRAWARANGHQINERGRVPAAVQA
                     AYDKAHA"
     gene            complement(2228..3130)
                     /gene="ligD"
                     /locus_tag="PLB86_08390"
     CDS             complement(2228..3130)
                     /gene="ligD"
                     /locus_tag="PLB86_08390"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HRC65193.1"
                     /translation="MPEPTDPAKVTVDAGGRRLVVSNLDKVIYPATETTKGEVLHYYA
                     RIAPVLLPHLAGRPVTRVRFPEGVEGLNFFEKNLPSGAPAWLPRVTLTHTSEPITYPM
                     VTGLDALTYLANLASLEFHVPQWQIGADGLPAFPDRLVVDLDPGPGAGLQECSIVALL
                     ARERLRALGLDPIPVTSGSKGMQLYAALPGSHASADIALVARTLAQELTAAHPDLVVW
                     KMTTSLRPGKVFFDWSQNVAAKTTISPYSLRGRPAPFVAAPRTWDEVQEGAATPGALA
                     QVTATEVLDRVERLGDLAAAALPR"
     gene            complement(3123..4067)
                     /gene="ligD"
                     /locus_tag="PLB86_08395"
     CDS             complement(3123..4067)
                     /gene="ligD"
                     /locus_tag="PLB86_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HRC65194.1"
                     /translation="MRPMLATPTRRVPTGPEWAHEVKWDGMRILADIHDGQVRLTSRT
                     ERDVTVAFPELAGLANEFEDMLLDGEAVVLRDGIPSFAALAERFHVTSPTKAAALAAR
                     APVTLMVFDLLRLYGVDLTGRSWTDRRATLERLELQNPHWTVPPTFTDGQALWEATRE
                     QGLEGIVSKRRSGRYAAGARTEDWVKLPHRTSQSVVIGGWRPETDQVGRLGAVLVGVP
                     GVGGLRYLGRVGSGIAGKTARALAADLAPLTSAGSPFVDEVPPEDASGTTWVHPVLVC
                     DVQALGLTDSRRLRQPAYRGLRPDLTPGDIDPAELPDA"
     gene            complement(4073..4552)
                     /locus_tag="PLB86_08400"
     CDS             complement(4073..4552)
                     /locus_tag="PLB86_08400"
                     /inference="COORDINATES: protein motif:HMM:NF020544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HRC65195.1"
                     /translation="MTWTARALTEEEHEVVRSNGKAAATLIEDEPPDPKRGVDLDTAW
                     HGIHWLLTGTAYDTETLAGQAIFGGDAVGTDLGHGPAHLIDPRTVKKISAALDALPAS
                     EVAARVDFEAMRGADIYPGFWDEQDVFHTWLRPRYKDLRKFYRRAARASSAVLVAIL"
     gene            4713..5708
                     /locus_tag="PLB86_08405"
     CDS             4713..5708
                     /locus_tag="PLB86_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HRC65196.1"
                     /translation="MRAMWKGAVSFGLVNVPVKLYSATENHDISFRQVHRADGGQIRY
                     QRVCSVCGDVVAFDDVAKGYETPDGQMVVLTDEDLAALPNQSSKEVAVAKFVPAGQID
                     PMLFDKPYYLEPEKAATKAYALLRDALLAADRVALVTVSIRQRMTMAVLRVHDDVIVL
                     QTLMWPDEIRAADQVNLGDTGHASDKELAMASMLVDSLSGDYDPAEFEDDYAGAVAAL
                     VDSKVTGAAVIEAPAAAADTGEVVDLLEALQRSVDRAKAARGEPVTPAVPAASSAASA
                     AAAPAQRAAAAKTTPAKTAATKATAAKTAAAKTTAAKTTAAKTAATKKTATRKAG"
     gene            5713..6597
                     /locus_tag="PLB86_08410"
     CDS             5713..6597
                     /locus_tag="PLB86_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HRC65197.1"
                     /translation="MPEGHTLHRLAGSLNGAFAGTLPTASSPQGRFAEGAAYLTGREL
                     HEAAAWGKHLFVEFEGAAYLHVHLGLIGTFDVTPFGLDESDAAPPPPVGAVRLRLATP
                     THVADLRGATVCELATPDQVDAVIARLGPDPLRADADPDRAWRRIAKSPRSIADLLMD
                     QAVLAGVGNVYRSEILFRHRVDPHRPGREIRPQTWKALWRDLLLLMPIGVQLGQIVTM
                     SDQVDAVLAGDFDPSAVPTSPTGVSAWRPNPHGALERRYYVYKRQGGACRVCGSRVRM
                     ADVAGRNLYWCGRCQRRR"
     gene            6647..7477
                     /locus_tag="PLB86_08415"
     CDS             6647..7477
                     /locus_tag="PLB86_08415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC65198.1"
                     /translation="MDDYLDINRANWDERAPAHAVSEGYAVRRLVSDPAALSGVVEFD
                     RPLLGDIAGLRGVHLQCHIGTDTLSLHRLGARMSGLDFSPAALHEARALAAEAGAEID
                     YVESTVDGAVAAFGAGGFDLVYTGIGALCWLPRIRDWAQTVATLLRPGGRLFIREGHP
                     MLWAMADPTGPESPVAVGYTYFEQPDAVVWDEPGTYVETEVEFKHNVTHSWNHGLGEI
                     VTALLDVGMVITGLVEHDSVPWEALPGLMVVDERGEWRLRENPERLAASYTLQAVKAP
                     "
     gene            complement(7533..8435)
                     /locus_tag="PLB86_08420"
     CDS             complement(7533..8435)
                     /locus_tag="PLB86_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC65199.1"
                     /translation="MTKKQSRVQSFSDAVVVGVDGHDSTHAALLWAAAEAGRRKTRLV
                     VMTIQEAYTHDLPFGAGVDEATAIADADTDVTARLAAQAARDAVPGLKVSTVRPEGQA
                     ASRLIKASKSAAMIVLGSHGRHKRAYAALGTTASTTAMHAKCPVVIVHPDRSAAHAAG
                     PAHVVVGVDGSRDSSLAIDAAADLAGKDGHVNVVNAWWFSMAESGSLVGGDVVEEDKI
                     RARHARGVRNIAAAAQKRHPKVTFTAVSVEGEPVAALVEQAKEADLLVVGARGRGGFG
                     GLLLGSNALKALAASPVPVAVIHK"
BASE COUNT         1377 a         2993 c         3096 g         1341 t
ORIGIN      
        1 tccgcaacac gggcaagctc tgcttcgcga ccctccagga gggggccggg acgcggctgc
       61 aggtcatgct cagcctcgcc gaagtgggtg aggcggccct cgccgactgg aaggccctgg
      121 tcgacctcgg cgatcacgtc gccgtcgaag ggcgggtcgt cagcagcaag cggggcgagt
      181 tgtcggtcat ggcgacctcc tggcagatgg cgtccaaggc gctgcggccg atgccgaccc
      241 tgcacaagga cctctccgag gagacgcggg tgcgtcagcg ctacgccgac ctcgtcgtgc
      301 gtcaggaggc ccgcgacatg gtccggttgc gggcgagggc cacccgcgcg atccgcgagg
      361 tgctcgacac gcagggctac ctcgagatcg agacgcccgt cctgcaactc gtccacggcg
      421 gcgccgcagc gcgccccttc cgcacgcatc tcaacgcctt cgaccagcag atgaccctgc
      481 gcatcgccct tgaactcaac ctcaagaagg cggttgtcgg cggcgtcgat cgggtctacg
      541 agatgggccg catcttccgc aacgagggcg tcgacggcac ccattcgccc gagttcacca
      601 tgttggaggc ctatcaggcc tacggcgacc agacgacgat cgccgcgctc atccgcgacc
      661 tctacctcgg cgtggccgac gccctcggca cgcgggtcgt gcagacgccc cgtggcgagg
      721 tcgacctcgg cggcgagtgg cgttggcttc cggtttacga cgcggtcagc gacgccgtcg
      781 gcgaggaggt caccctcgcc acgcccgtgg agacgctgcg ccggtatgcc gccgcgcacg
      841 acgtcccgct cgaccccgcc tggggggcgg acaaggtcgt cctggagttg ctgtccgagt
      901 tggtcgagcc aaaggtcctc cagccgacct tcttctgcga ctatccggcg atcgcccagc
      961 cgttggcgcg cccccaccgc tcagtgccgg ggctcatcga ggcctgggac ctcatcatcg
     1021 cgggggtcga gcgaggcacc ggattctccg agctcatcga cccggtgatc cagcgccagg
     1081 tcctcgaagc tcagtcgctg ctcgccgccg ggggagaccc cgaggccatg gcgctcgatg
     1141 aggacttcct gcgggccctg gagttcggtg cccctccgat gggcggtctg gggctcggaa
     1201 tcgaccggct cgtcatgctc ttcaccggcg cctcgatccg cgaaaccatc ctcttcccgc
     1261 tcttgaagcc ggaggactga ccatgtggga cgcgatctac ccctatctcg ccgccatcct
     1321 gccgacgatc ggcctgctgg tgatcgccta tgtcgtcttc aagacgatct tcgaggccga
     1381 ccggcgcgaa cgaaaagccg ttgcggaatg ggaacgcagc cacgacgcgt cgccgatcag
     1441 ttctaccgat gacgagtcgc gaccggcgac ttagtcgtcc gcggccggct ccgcgacctt
     1501 cggacaattc tgccgggagg cctccgatgc atcgaggtca cgacccattc accgagatca
     1561 aaggggacgg gccaggatca ccggaggcga tgaaccggtg accgacgcgc aacgaccatc
     1621 gggcgccacg gacccccttc ctggagtttg tcgatttccg cgtgttacga tcacccgcgg
     1681 tgatcgacga agtttcgcgg gattcttcgc caaccgccag gtaagacggc acggagggtt
     1741 agccttgacc cacggtccgt agagtgacat tcaacaaagc cgggcaaccg gcgttttcct
     1801 ccgcagcagt tccctcatga atggagcacc caccgtggcc aagcgtgttc aggtcgtcct
     1861 cgaagacgac gttgacggca gccccgcaga cgagaccgtg tctttcggtc tcgacggcgt
     1921 ttcttacgag atcgaccttt cggccgcgaa tgcctcggcg ttgcgtgacc agctcgccaa
     1981 atggatcggt gctgcccagc gcaccggtgg tcggcgggcc gcagggcgtc gcgccggggg
     2041 ctcggcgcga cggactgacg tttcagccgt tcgggcgtgg gcacgggcca acggccacca
     2101 gatcaacgag cgtggccggg tgccggcggc ggtccaggcg gcctacgaca aggcacacgc
     2161 ctgatcgagc ggttccgcac ggtccggacg ggccgcactc cttcgggagg gcggcccgtc
     2221 cgctgtttca ccggggcagc gccgcagcgg caagatctcc gaggcgctcg acccgatcga
     2281 gcacctcagt ggccgtgacc tgagccaggg ccccgggagt cgccgcgccc tcctgcacct
     2341 cgtcccacgt gcgcggagca gcgacgaacg gtgccggtcg gccgcgcaat gaatagggcg
     2401 agatcgtcgt cttggccgcg acattctggc tccagtcgaa gaacaccttg cccggtcgca
     2461 acgaggtcgt catcttccag acgacgaggt cgggatgggc ggccgtcaat tcctgggcca
     2521 acgtccgggc gaccagcgca atgtccgccg acgcgtggga gcccgggagg gcggcataca
     2581 actgcatgcc cttggacccg ctggtgacgg ggatcgggtc caaccccagg gcccgaagcc
     2641 gctcccttgc cagcagggcg acgattgagc actcctgtag ccctgctccg gggccgggat
     2701 cgaggtcgac gacgagccga tcgggaaagg ccggcagccc gtccgcgccg atctgccatt
     2761 gcggcacgtg aaattccagc gaggcgaggt tggccaggta ggtgagcgcg tccagccccg
     2821 tcaccatcgg ataggtgatg ggctcgctgg tgtgcgtgag ggtgacccgg ggcagccatg
     2881 ccggcgcccc cgacggaagg ttcttctcga agaagttgag gccctcgacc ccctcgggga
     2941 accggacccg agtgactgga cggcccgcga gatgtggcag cagcaccggc gcgatccgcg
     3001 cgtaatagtg caacacctcg cccttggtcg tctcggtcgc cgggtagatg actttgtcga
     3061 ggttcgatac gacaagtcgc cgtccaccgg catcgacggt cacctttgcg gggtcggtcg
     3121 gctcaggcat cgggcaactc ggccgggtcg atgtcaccgg gcgtgaggtc cggacgcaat
     3181 cctcggtatg ccggttggcg cagtcggcgg ctatcggtga gtcccagcgc ctggacgtcg
     3241 cagacaagca ccggatggac ccaggtggtg cctgaggcgt cctctggggg cacctcgtcg
     3301 acgaacgggc tcccggcgga ggtcaggggc gccaggtcgg cggcgagcgc cctggcggtc
     3361 ttgccggcga tccccgaccc gacccgcccc aggtaacgca gccctccaac ccccggcacg
     3421 ccgacgagga cggccccgag ccggcctacc tggtcggtct ccggtcgcca gcctccgatg
     3481 acgaccgatt gcgaggtgcg gtgcggcagt ttgacccagt cctcggtgcg ggcgccggcg
     3541 gcataccgac cagatcggcg cttgctgacg atgccttcca ggccctgctc ccgggtggcc
     3601 tcccacagcg cctgcccgtc ggtgaaggtg ggcggcaccg tccagtgcgg gttctgaagt
     3661 tccagccgct cgagggttgc ccggcgatcg gtccacgacc ggccggtgag gtcgaccccg
     3721 tagaggcgca agaggtcgaa gaccatcagg gtcaccggcg cgcgggcggc cagggccgca
     3781 gccttcgtcg ggctggtcac gtggaaccgc tcggccagag ccgcaaacga gggtatgccg
     3841 tcgcgcagca ccaccgcttc gccgtcgagc agcatgtcct cgaattcgtt tgccagacct
     3901 gctagttcgg ggaaggcgac ggtgacatcc cgttcggtcc gagacgtgag gcgcacctgg
     3961 ccgtcgtgga tgtcggcgag gatccgcatc ccgtcccact tgacctcatg ggcccactcg
     4021 ggacccgtcg gcactcgacg ggtgggcgtg gcgagcatcg ggcgcatgcg gctcagagga
     4081 tggcgacgag aacggcggag ctggcccgcg ccgcccgccg atagaacttg cgcaagtcct
     4141 tgtaacgcgg tcgcaaccac gtgtggaaca cgtcctgctc gtcccagaac ccggggtaga
     4201 tgtcggcgcc ccgcattgcc tcgaagtcga cccgggcggc gacctccgag gccgggaggg
     4261 cgtcaagcgc cgcactgatc ttcttgaccg tgcgcgggtc gatgaggtgg gcgggaccgt
     4321 gccccaggtc ggtccccact gcgtcccccc cgaaaatcgc ctggcccgcg agggtctcgg
     4381 tgtcgtaggc cgtgcccgtc agcagccagt ggattccatg ccatgcggtg tcgagatcga
     4441 caccgcgctt gggatcagga ggttcgtcct cgatgagggt cgcggcggcc ttgccattgg
     4501 agcgcacgac ctcgtgctcc tcttcggtca gcgcacgcgc cgtccacgtc atcaccacag
     4561 gggcgattat gccgtccttg ggcaaacggc agcgtcccgc cgggtgatcg ggaggtggac
     4621 gggcggcata cccgatggac gtcggcgttg tggggaggcg agcggtgagc catgctggtg
     4681 acgtacgtgg ccactgggaa gggtggggtt cgatgcgggc gatgtggaag ggcgcggtgt
     4741 ccttcgggct ggtcaatgtg cccgtcaagc tctactcggc gaccgagaac catgacatct
     4801 ccttccgcca ggtccaccgg gccgacggcg ggcagatccg ctaccagcgg gtctgcagcg
     4861 tctgtggtga cgtcgtggcc ttcgacgacg tcgccaaggg ctacgagacg cccgacgggc
     4921 agatggtcgt gctcaccgac gaggacctgg ccgcgctgcc caaccagtcg agcaaggagg
     4981 tcgcggtcgc caagttcgtg ccggccggtc agatcgaccc gatgctcttc gacaagccgt
     5041 attacctcga gcccgagaag gccgccacca aggcgtatgc gctgttgcgc gacgcgctgc
     5101 tggccgccga ccgggtggcc ctggtgaccg tgtcgatccg tcagcggatg accatggcgg
     5161 tgctgcgggt ccacgacgac gtcatcgtcc tgcagaccct catgtggccc gacgagatcc
     5221 gggcggccga ccaggtcaac ctcggcgaca ccgggcatgc cagcgacaag gaactggcga
     5281 tggcgagcat gctggtggac tcgctgtccg gcgactacga cccggccgag ttcgaggacg
     5341 actacgccgg ggcggtggcc gccctggtcg actccaaggt gacgggcgcc gccgtgatcg
     5401 aggccccggc ggctgctgcc gacacgggcg aggtcgtcga cctcctggaa gccctacagc
     5461 gcagcgtcga ccgagccaag gccgctcgcg gcgagccggt aaccccggcc gttccggccg
     5521 cctcgtccgc cgcctcggcc gccgcagcgc cggcgcagag ggctgcggca gccaagacca
     5581 cgccagcgaa gaccgctgcc accaaggcga cggcggccaa aacagcggca gccaagacga
     5641 cggcagccaa gacgacggca gccaagaccg ctgcaaccaa gaagaccgcg acccgcaagg
     5701 ccggctgacc ggatgcctga aggccatacc ctgcaccggc tggcggggtc actcaacggt
     5761 gcgttcgctg gcactctgcc cacggcgtcg agcccgcagg gacgcttcgc cgagggagcg
     5821 gcatacctca ccggtcgcga gttgcacgag gcggcggcgt gggggaagca cctgttcgtc
     5881 gagttcgagg gcgcggccta cctgcacgtg catctcggtc tcattggcac cttcgacgtg
     5941 accccgttcg gcctggacga gtcggacgcc gcgccgccgc cgcccgtggg tgcggtccgg
     6001 ctgcgattgg ccacgccgac ccatgtcgcg gacctgcgcg gcgcaaccgt gtgcgagctg
     6061 gccacgcccg atcaggtcga cgcggtcatc gcccggctcg ggcctgaccc gttgcgggcc
     6121 gacgctgacc cagaccgggc ctggcgacgg atcgccaaga gcccgcgcag catcgccgac
     6181 ctgctcatgg accaggcggt cctcgccggc gtgggcaacg tctatcgcag cgagatcctc
     6241 ttccggcatc gcgtcgaccc ccaccggccc ggccgcgaga tccggccgca gacgtggaag
     6301 gccctgtggc gagatctgtt gctgctcatg cccatcggtg tccaactcgg ccagatcgtc
     6361 acgatgagcg atcaggtcga cgcggtgctg gcgggagact tcgacccgag cgcggtgccg
     6421 acctcgccca ccggggtgtc ggcttggcgt cccaacccgc acggggcgct cgagcggcgc
     6481 tactacgtct acaagcgtca gggcggggcg tgtcgggtct gtggctcccg ggtgcggatg
     6541 gccgacgtgg ccggtcgcaa cctgtactgg tgtggccgct gccaacgccg ccgctgagtc
     6601 gtaatcgtcg ctgagcccgc gggcggcata cctagactcg gcgaccatgg acgactacct
     6661 ggacatcaac cgcgcgaact gggacgagcg cgctcccgca cacgcggtct ccgaggggta
     6721 tgccgtgcgc cggctggtca gcgacccggc cgccctctcc ggcgtggtcg agttcgaccg
     6781 cccgctgctg ggcgacatcg cgggtttgcg cggagtccac ctgcagtgcc acatcggcac
     6841 cgacacgctg tccctgcacc gactcggggc ccggatgagc ggcctggact tctcacccgc
     6901 agccctccac gaggcgaggg ccctggccgc cgaggcgggc gccgagatcg actatgtcga
     6961 gtcgacggtc gacggggcgg tggccgcgtt tggcgccgga ggcttcgacc tggtctatac
     7021 cggcatcggg gcgctgtgct ggctgccgcg gatccgcgac tgggcccaga cggtggcgac
     7081 cctgctgcgc ccaggcggtc ggctgttcat ccgcgagggc cacccgatgc tgtgggcgat
     7141 ggccgacccg accgggccgg agagccccgt ggcggtcggt tacacctact tcgagcagcc
     7201 cgacgcggtg gtgtgggacg agcccgggac ctatgtcgag accgaggtcg agttcaagca
     7261 caacgtcacc cactcgtgga accacgggct cggcgagatt gtcacggcgc tgttggacgt
     7321 cgggatggtg atcaccgggc tcgtggagca cgacagcgtt ccgtgggagg cgctgcccgg
     7381 cctcatggtc gtcgacgagc gcggcgagtg gcggctgcgg gagaaccccg agcgcctagc
     7441 cgccagttac acgctgcagg ccgtcaaggc accctgacgg aatggcggtg gggcggcgcc
     7501 cgcgcaggca ccaccccacc tcgtccatat gcctacttgt ggatcaccgc gacggggacg
     7561 ggggaggcag ccagggcctt gagggcgttg gagccgagga gcagcccgcc gaagccgccg
     7621 cgcccacggg ctccgacgac gaggaggtcg gcctccttgg cctgctcgac gagcgccgcg
     7681 acgggctcgc cctcgaccga caccgcggtg aaggtcacct tggggtgacg cttctgggca
     7741 gcggcggcga tgttgcggac cccacgagcg tgccgagccc ggatcttgtc ctcctcgacc
     7801 acgtcaccgc cgacgaggga gccgctctca gccatgctga accaccaggc gttgacgacg
     7861 ttgacgtggc cgtccttgcc ggccaggtcc gcggcggcgt cgatggccag cgaggagtca
     7921 cgcgagccgt cgaccccgac gacgacatgc gccggcccgg ccgcgtgggc ggcggaacgg
     7981 tcggggtgaa cgatgacgac cgggcacttg gcgtgcatcg ccgtggtgga ggcggtggtg
     8041 ccgagcgcgg catacgcccg cttgtgtctg ccgtggcttc cgagcacgat catggcggcc
     8101 gacttggagg ccttgatgag gcggctggcg gcctggccct cggggcgcac ggtgctcacc
     8161 ttgaggcccg gaacggcgtc gcgtgcggcc tgggcggcca ggcgtgccgt gacatcggtg
     8221 tcggcgtcgg cgatcgcggt ggcctcatcg acccctgctc cgaagggcag gtcgtgggtg
     8281 taggcctcct ggatcgtcat gaccaccaag cgggtcttgc gacggccagc ctccgcggcc
     8341 gcccacagca gcgcggcatg cgtgctgtcg tggccatcca cgccgaccac gacagcatcg
     8401 gagaacgact ggactctgga ctgtttcttc gtcatgcagc cattatctga ttgtcagtga
     8461 tctgacaact gggtctgagg gcctgatcgg ctcagatgtg acgagcggaa cgtctgggct
     8521 gggggaggtg gggaggtccg ctcagagcga acacgggagt ggcgattggg gggaacaggg
     8581 cggcataccg gggcgttgcc agaggggtca cccccgtaac gtgccgttca ccacgaaggc
     8641 cgcgaagtgt gacacggtta ccatcgaggc aactcggaac gacggcagga gtgttcaacg
     8701 atgttcgaac ggttcacgga ccgcgcccgg cgagtggtcg tgctcgcgca ggaagaggcg
     8761 cgcatgctca accacaacta catcggcacc gagcacatcc tcctcgg
//
LOCUS       DAORPW010000092         8715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1956_length_8715_cov_587.711547, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000092 DAORPW010000000
VERSION     DAORPW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1956_length_8715_cov_587.711547"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLB86_08425"
     CDS             <1..624
                     /locus_tag="PLB86_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRC65200.1"
                     /translation="KINAAFAFGGARLLTETVQQATGLHIDGYLEIGFGGFARVVDSL
                     GGVDICVARDMKDELAGIDLKAGCQVLDGKNALGYVRSRYSDPRGDLGRAERQRQFLG
                     AVMKKAATPATVLNPSRYLAFADAASAGLIVGSDTSLGDAVAIMQALRAVGNGEGLSL
                     QVPIETAALQTKNAGVAVKWNTTQAKALFTALRNDESLTAPPPGTAP"
     gene            complement(655..2499)
                     /locus_tag="PLB86_08430"
     CDS             complement(655..2499)
                     /locus_tag="PLB86_08430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC65201.1"
                     /translation="MASDDPAAPPGIGGVVAVVVTWNRRELLTRCLDAVLGQTHRPSR
                     VVIVDNASDDGTAALLADRYAALPGVQIVRTIENIGGAGGFALGLEFALGGAGDTAWL
                     LDDDTVPTAGALQALVGARTAYRTLTGTTPVLVASRAVWTDGRDHPMNTPRPKPGASA
                     DEATAAAAVGCIPIRTASFVSILVDLDRTREVGLPEAGFFLWNDDFEFTARLVRGHRA
                     LYCPGSTVVHETKKFGGADADPGPRFHLEVRNKVWTFGRSRGLSAPEKVLYGGATARR
                     WARTIMRSTDRSTLIRGLGQGLRAGLSGGPRPTSVVLTEALGPDPAVGSLQPRPPQPR
                     REDGFAVLMSVYAGDRADFFEAAFRSVTADQTVPPDQVVLVIDGPLPQALNSRIEEVV
                     AATQVPVTLVRLPVNGGLGPALRAGLAHSEHELVARMDADDVSLPTRFEVQVPMVRGG
                     LDLVGSALLEFEDDPQVISRRRAVHTDPAQIQTGARFRQTLNHPSVVFRASAVEAAGG
                     YQDLPSLEDYLLFARMIHAGARVGNVAEPLVLYRVGAGSYARRGGLRLLRSELALQRE
                     FRRTGFTTRLQMMRNLVIRGGYRVAPEGLRKAGYGTFFARRDRGSGRG"
     gene            complement(2585..3754)
                     /locus_tag="PLB86_08435"
     CDS             complement(2585..3754)
                     /locus_tag="PLB86_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC65202.1"
                     /translation="MWRVNPDFDAFQLSDSHRQLRAAVRELAEAQIEPYAADVDENAR
                     YPQEAHDALVASDFFAPHVPEEYGGAGADALAVCIIIEEISRVCASSSLIPAVNKLGS
                     MPLILAASEDLKHRYLTPMAEGKSGFCYGLSEREAGSDTSGMTCTATEQPDGSWILDG
                     HKAWITNAGVNDFYTVLAVTDPEGRRGANISAFVVEKSDPGFTFSEKERKLGIKGSPT
                     RELWFDNCTIPGDRMIGSRGEGLKIALRTLDHTRITIGAQAVGIAQGALDKAVTYVKE
                     RQQFGKNIAEFQGVQFMLADMAMELEAARQLVYVAAAKSERGDRDLSFFGAAAKCFAS
                     DVAMKVTVDAVQLMGGMGYTRDFPVERMMRDAKITQIYEGTNQIQRIVMARSVLA"
     gene            3889..4170
                     /locus_tag="PLB86_08440"
     CDS             3889..4170
                     /locus_tag="PLB86_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HRC65203.1"
                     /translation="MRAIWNGTVLAESDDTVVVEGNHYFPTAALRHDLVRPSDTHTVC
                     PWKGTASYFSVEVDGQVNEDAVWYYPDPKPKAAMVADRVAFWKGVTVSE"
     gene            complement(4190..5080)
                     /locus_tag="PLB86_08445"
     CDS             complement(4190..5080)
                     /locus_tag="PLB86_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRC65204.1"
                     /translation="MSWTVDEIPDQSGRVVLITGANSGIGLESASVLADRGAHVILGC
                     RDPHKGAAAQRQIAGSTEVLTIDLSSLASVSEAAEALGGRQIDVLVNNAGIMAVPQEA
                     SVDGHELQFATNVLGPFALTSLLLDQITDRVVWVSSMMHRAGKLDLADPSFRHHRYNA
                     WRAYSASKLADLMLSYELQRRLTLAGSTVRSYAAHPGYAQTGLQRHTAVLSWPMVHQL
                     ESLLKVAQSADQGALPILYAATAPDLPPGSYVGPAGFGEMSGPPRIVGSSAASHDRDA
                     QRTLWSLCEGLTGTSPGLPG"
     gene            complement(5085..6107)
                     /locus_tag="PLB86_08450"
     CDS             complement(5085..6107)
                     /locus_tag="PLB86_08450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRC65205.1"
                     /translation="MRFGAFVPQGWRLDLVGIDPALHWGVMDGLARRFDAVHAWESLW
                     VYDHFHTVPKPSDEATHEAWTLMAAFAASTNRIRLGQMCTCMAYRNPAYLAKVAATVD
                     IISGGRVEMGIGAGWYEHEWRAYGYGFPEARDRLGALRDGVEIFRQMWTTGSATYAGT
                     YYTVDGAICAPRPLQGTAAPGSPANGIPMWVAGGGEKVTLKIAAKYADYTNFFGDLEE
                     FTRKNEILKAHCAALGRDEAEIVRSINVNVFIGETEVELNDRLEWYADHRIRVCGLPE
                     AAVRREIDGLHGSVGCGTPEQVVERLAALRRAGLGYTIAYFADAAYDTTSIDLFERAV
                     IPELKG"
     gene            6221..6661
                     /locus_tag="PLB86_08455"
     CDS             6221..6661
                     /locus_tag="PLB86_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRC65206.1"
                     /translation="MTAHHNHLEVIRALLTTPATWAVVGLSDNEGRTAYRIAQWLRAR
                     LDMQVLPIHPSAPFDPPVLGSPGYAALAAIPDGVRVAVVDCFVRSELVGAVVDEAIAQ
                     RERLGIGAVWLQLGVIDEAAAARAEAAGLAVVMDTCPRIEWPRL"
     gene            complement(6871..7425)
                     /gene="purE"
                     /locus_tag="PLB86_08460"
     CDS             complement(6871..7425)
                     /gene="purE"
                     /locus_tag="PLB86_08460"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRC65207.1"
                     /translation="MSERRSAATSSGDSLGDDPVVGIVMGSDSDWPVMALAAEALDEF
                     GVAYEVDVVSAHRMPEEMIAYGKGAAERGLRVIIAGAGGAAHLPGMLASVTPLPVIGV
                     PVPLAYLDGMDSLLSIVQMPAGVPVATVSVGGARNAGLLAARILGAGEGAEAARMRAE
                     MVRFQGRLRDQAHAKGAALRASRA"
     gene            complement(7418..8659)
                     /locus_tag="PLB86_08465"
     CDS             complement(7418..8659)
                     /locus_tag="PLB86_08465"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRC65208.1"
                     /translation="MTSPRRAPGGFPVVGVIGGGQLARMLQAPAVALGVQLSVLAEAV
                     DASAALVIPSAPVGDHADLDAVCRFAASCDVVTFDHEHVPADVLAALEADGVPLHPSP
                     AALRFAQDKLAMRHRLTEIGVACPRWAQARTRADIETFAAVVGWPIVAKTPRGGYDGK
                     GVRVLRGPEDLDVLDAPGEWLEQVGAPGALADGILVEELVPFERELAVLVARSPSGQA
                     SAWPVVQTVQTDGICTEVLAPAPDLDPATAAEATHAALRIAGALGVTGVLAVEMFQVA
                     RAAPGHGDGPAVGERPAYLVNELAMRPHNSGHWSIDGAVTSQFEQHLRAVLDLPLGDP
                     RPVASWTVMANVLGGDYAEIYPAYRHVMARDPGAKVHMYGKGVRPGRKIGHVNVSGPD
                     LADARARAQHAADYLQGVIDE"
BASE COUNT         1403 a         3031 c         2993 g         1288 t
ORIGIN      
        1 aaaatcaacg cagcgtttgc tttcggcgga gctcggttgc tcaccgaaac ggtccaacag
       61 gccacgggtc tgcacattga cggctacctt gagatcggtt tcggagggtt cgctcgggtc
      121 gtcgacagcc ttggcggagt cgatatatgc gtggcgcgtg atatgaaaga cgaattggcc
      181 gggatcgacc tcaaggccgg atgtcaggtc ctggacggca agaacgccct cggttacgtg
      241 cgatcgcgtt acagcgaccc ccggggcgat ctgggccgag ccgaacgtca gcgccagttc
      301 ctcggggcgg tgatgaagaa ggccgccacc cccgccaccg tcctcaatcc cagccgctac
      361 ctggcttttg ccgacgccgc gagtgccggg ctgatcgtgg gttccgacac ctcgctcggc
      421 gacgcggttg ccatcatgca ggcgctgcgg gcggtgggaa acggtgaagg cctgtccttg
      481 caggttccga tcgagacagc agcgctccag acgaagaacg cgggggtggc tgtcaagtgg
      541 aacaccaccc aggccaaggc cctcttcacc gcgctacgca acgacgagag cctgactgcg
      601 ccgccccctg gcactgctcc ctgaccccgt gcgccagcgc ccgcgagacg cccctcagcc
      661 ccgtcctgaa ccgcggtccc gacgagcgaa gaaggtgccg tagcccgcct tgcgcagccc
      721 ttcgggagca acccgatagc cgcccctaat gaccaggttg cgcatcattt ggagccgcgt
      781 cgtgaaaccc gtgcgccgga actcccgttg cagagcaagt tcggaccgca acaaccgcag
      841 cccaccgcgg cgggcgtagg accccgctcc gacgcgatac agcacgaggg gttcggcgac
      901 attgccgacc cgcgccccgg catggatcat ccgggcgaac agcaggtagt cctcaaggga
      961 gggaagatcc tggtagccgc ccgcagcctc gacggcactg gcccggaaga cgaccgacgg
     1021 atggttgagg gtctgccgga accgagcccc ggtctggatc tgggccggat cggtgtggac
     1081 cgcacggcgt cggctgatga cctgtgggtc gtcctcgaac tcgagcaggg ccgaccccac
     1141 gaggtccagg cccccacgca ccatcggcac ttggacctcg aatctggtgg ggagcgacac
     1201 gtcgtcggca tccatgcggg cgaccaactc gtgctcggag tgggccagcc cggctcgcag
     1261 cgccgggccg aggcccccgt tgaccggtag gcgaaccaac gtaaccggca cctgcgtggc
     1321 cgcgacgacc tcctcgattc gggaattcaa tgcttggggc aacggtccgt cgatgacgag
     1381 gacgacctgg tccgggggga cagtctgatc cgccgtgacg gatcggaagg ccgcctcgaa
     1441 gaaatccgcc cggtcgccgg cgtagacgct catgaggaca gcgaagccgt cctcgcgtcg
     1501 gggctgtggc ggccgcggct gcaacgagcc gaccgccgga tcgggtccca gtgcctcggt
     1561 gagcacaacg ctggtgggtc ggggcccacc gctcagaccg gcgcgcaggc cttgacccag
     1621 gccccggatc agcgtggacc ggtcggtgga cctcatgatg gtccgagccc aacggcgcgc
     1681 agtggcaccg ccgtagagga ccttctccgg agcgctgagc ccacgactgc gcccgaacgt
     1741 ccagactttg ttacgcactt cgaggtggaa gcgcgggccg gggtcggcgt cggcgccgcc
     1801 gaacttcttc gtctcgtgga cgacggtcga cccagggcag tagagggccc ggtgcccacg
     1861 gacgagcctg gcggtgaact cgaagtcgtc attccacaga aagaatcccg cttccggcaa
     1921 gcccacctca cgagtgcggt ccaggtcgac gaggatggag acgaacgacg ccgtccgaat
     1981 cgggatacat cccaccgcgg cggcggccgt tgcctcgtcg gcggacgctc cgggtttggg
     2041 ccgcggggtg ttcatcggat ggtctcggcc atcggtccac actgcgcgac tggccaccag
     2101 gaccggggtg gttccggtga gcgtccggta tgccgtccgc gcacccacca gggcctgcag
     2161 ggctcccgcc gtggggaccg tgtcatcgtc gagcaaccag gcagtgtcac cggcgccgcc
     2221 caaggcgaac tcgagcccga gggcaaaacc gcccgcaccg ccgatgttct cgatggtccg
     2281 gacgatctgg acgcccggca gggcggcata ccgatcagcg agcagcgcag cggtgccgtc
     2341 gtcagacgcg ttgtcgacga tcacgacccg cgacggtcga tgggtctgac ccagcaccgc
     2401 atccaggcag cgggtcagca gttcccgccg gttccacgtg acgacaacgg cgacgacccc
     2461 gccgatgcct gggggtgcgg cggggtcgtc tgaagccata cgcgacatcc tagggagccg
     2521 agaactgatg gccggaccac gcccggcaga cccggatggc cccgcctatc ggtcaaccgg
     2581 atcgtcaggc caagacgctg cgcgccatga cgatgcgctg gatctggttg gttccttcgt
     2641 agatctgggt gatcttggcg tcgcgcatca tccgctcgac ggggaagtcc cgggtgtagc
     2701 ccatgccgcc catcaactgg acggcgtcga cggtcacctt catggccaca tcgctggcga
     2761 agcacttggc cgcggcaccg aagaaggaca ggtcgcggtc accgcgctcg gacttggccg
     2821 ctgcgacata gaccaactgc cgcgccgcct caagctccat cgccatgtcg gcgagcatga
     2881 actgcacacc ctggaactcg gcgatgttct tgccgaactg ctgacgttcc ttgacgtagg
     2941 tgaccgcctt gtccagcgcg ccctgggcga tgccgacggc ctgggcaccg atggtgatgc
     3001 gggtgtggtc gagcgtgcgc agcgcgatct tcaggccctc accgcgggag ccgatcatcc
     3061 ggtcgcccgg aatcgtgcag ttgtcgaacc acaactcacg ggtcgggctg cccttgatgc
     3121 cgagcttgcg ctccttctca ctgaaggtga agcccgggtc gctcttctcg acgacgaagg
     3181 ccgagatgtt ggcgccgcgg cgcccctcgg ggtcggtgac ggcgagcacc gtgtagaagt
     3241 cgttgacacc ggcgttggtg atccacgcct tgtgtccgtc gaggatccac gacccgtccg
     3301 gctgctcggt cgccgtgcag gtcattcccg acgtgtccga cccggcctcg cgctcggaca
     3361 ggccgtaaca gaacccgctc ttgccctcgg ccatcggggt caggtagcgg tgtttgaggt
     3421 cctcgctcgc ggcgaggatg agcggcatac tcccgagttt gttgactgcg gggatgaggg
     3481 aactggacgc gcagacccgc gagatctcct cgatgatgat gcacacggcc aacgcgtcgg
     3541 caccggcgcc gccgtactcc tccggcacgt ggggtgcgaa gaagtcggac gccacgaggg
     3601 cgtcgtgagc ctcttgcggg tagcgggcgt tctcgtcgac atccgcggca tacggctcga
     3661 tctgcgcctc ggcgagctcg cgcactgcgg cgcgcagctg acggtgggag tcggaaagct
     3721 ggaaggcgtc gaagtccggg ttcacgcgcc acacgctact ccggggtatg ccgcgccggc
     3781 cggcggtgcg gcgaggttca ccctcgtgtg cgaaggaaca cctcgaccga gctcggcgtt
     3841 cggactagca cgtcacccgc ccgttgacgc cacccgaagg agcccctgat gcgtgcgatc
     3901 tggaacggaa cggtcctggc agagagtgac gacacggtgg tcgtcgaagg caaccactac
     3961 ttcccgacgg cggcgctgcg ccacgacctg gtgcggccgt cggacaccca caccgtgtgc
     4021 ccatggaagg gcacggcgag ctacttctcg gtggaggtcg acggccaggt caacgaggac
     4081 gccgtctggt actaccccga ccccaagccg aaagcggcca tggtcgccga ccgggtggcc
     4141 ttctggaagg gcgttaccgt cagcgagtga cctgtcagcg agtgcccggt cagccgggca
     4201 gcccggggga cgtcccggtg agcccctcgc acagcgacca cagggtgcgt tgggcgtcgc
     4261 gatcgtgcga ggcggccgac gagccgacga tccgcggcgg gcccgacatc tcaccgaacc
     4321 ctgcgggccc gacatacgag cccgggggca ggtcgggtgc cgttgcggca tagaggatcg
     4381 gcagggcgcc ctggtcggcc gactgcgcca ccttgagcag gctctccagt tgatgcacca
     4441 tcggccagct caggacggcg gtatgccgtt gcagccccgt ttgcgcgtaa ccggggtgcg
     4501 cggcatacga gcggaccgtc gagccggcga gcgtgagccg acgttgcaac tcgtaggaga
     4561 gcatgaggtc ggcgagtttg gacgcggagt aggcccgcca cgcgttgtag cggtggtggc
     4621 ggaagctggg gtcggcgagg tcgagcttgc cggcccggtg catcatcgag ctgacccaga
     4681 cgacccggtc ggtgatctgg tcgagcaaca ggctggtgag cgcgaacggc ccaagcacat
     4741 tggtcgcgaa ctgcaactcg tgcccgtcga ccgacgcctc ctgcggcacc gccatgatgc
     4801 cggcgttgtt gaccaggacg tcgatctggc gcccacccag cgcctcggca gcctcgctga
     4861 ccgacgccaa cgacgacagg tcgatcgtca gcacctcggt gcttccggcg atctggcgct
     4921 gcgcggccgc acccttgtgc gggtcacggc acccgaggat gacgtgcgcg ccgcggtcgg
     4981 ccaggacgct ggcggactcc aggccgatgc cactgttggc accggtgatg aggacgaccc
     5041 gcccgctctg gtcggggatc tcgtcgacgg tccacgacat gggttcagcc cttcaactcg
     5101 gggatgacgg cgcgctcgaa caggtcgatc gacgtcgtgt cataggccgc gtcggcgaag
     5161 taggcgatgg tgtagcccag cccggcccga cgcagtgcgg ccagccgctc cacaacctgc
     5221 tccggcgtgc cgcagccgac cgagccgtgc agcccgtcaa tctccctgcg gaccgccgcc
     5281 tccggcaggc cgcagacccg gatccggtgg tcggcatacc actcgagccg gtcgttgagc
     5341 tcgacctcgg tctcgccgat gaagacgttg acgttgatcg agcggacgat ctcggcctca
     5401 tcgcgcccga gcgcggcgca gtgggccttg aggatctcgt tcttgcgagt gaactcctcg
     5461 aggtcgccga agaagttggt gtagtcggca tacttcgcgg cgatcttcag cgtcaccttc
     5521 tcgccgccac cggccaccca catgggtatg ccgttcgccg ggctccccgg agcagcggtg
     5581 ccttgcagcg ggcgcggcgc gcagatggcg ccgtcgacgg tgtagtacgt gccggcatac
     5641 gtcgcgctgc ccgtggtcca catctgccgg aagatctcga cgccgtcgcg tagggcgccg
     5701 agccggtcgc gggcctcggg gaagccgtaa ccgtaggcgc gccactcgtg ttcgtaccag
     5761 ccggcgccga tgcccatctc aacgcggcca ccgctgatga tgtcgaccgt cgccgcgacc
     5821 ttggcgaggt aggcggggtt gcggtaggcc atgcaggtgc acatctggcc gagccggatc
     5881 cggttggtcg aggccgcgaa ggccgccatg agggtccagg cctcgtgggt cgcctcgtcg
     5941 gacggcttgg gcaccgtgtg gaagtggtcg tagacccaga gcgactccca cgcgtggacc
     6001 gcgtcgaacc gccgcgccag cccgtccatg acgccccaat gcagggccgg atcgatgccc
     6061 accagatcca gccgccaacc ctgtggaacg aacgctccga accgcatgtc ctgcctcccg
     6121 gtcgtgacct cggcgcccgg cgcaacggct gcgcgctggg cctgaccgtc atcatgtcac
     6181 cgaccgtcga caccgacgca gccgtgtgag actggcgccc atgaccgctc accacaacca
     6241 cctcgaggtc attcgcgcgc tgctcacgac cccggccacc tgggcggtgg tcggcctgtc
     6301 cgacaacgag gggcgcacgg cataccggat cgcgcagtgg ctgcgcgccc gactggacat
     6361 gcaggtgctg ccgatccatc cgagcgcgcc gttcgacccg cccgtgctcg gttcgccggg
     6421 gtatgccgcg ctcgccgcga tccccgacgg ggtccgcgtg gccgtcgtgg actgcttcgt
     6481 gcggtcggag ttggtggggg ccgtcgtcga cgaggcgatc gcccagaggg agcggttggg
     6541 gatcggcgcg gtgtggctgc aactcggggt gatcgacgag gcggcggcgg cccgagccga
     6601 ggctgccggc ctggccgtcg tcatggacac ctgcccccgg atcgaatggc cccgcctcta
     6661 gcccgctgcc cccacgcgcc ccacgactcc cacgaccccc gccacgcgcc ccacgtgccc
     6721 cacgcgcccc acgactcccg ccacggagat agcgctcgca cggtcatctt tccgtcaact
     6781 caggcccgaa atgaccccac agatgaccgt gcgagcgcta tctccgggtc aggatggggg
     6841 tgggagacgc gcgaatacag atgtatgccg tcaggctcgg gaggctcgta gcgcggcgcc
     6901 cttggcgtgg gcctggtcgc gcaaccgccc ctggaagcgc accatctcgg cgcgcatccg
     6961 agccgcctcg gcaccctcac cggcgccgag gatccgcgcc gccaacaggc cggcgttgcg
     7021 tgcgccgccc accgacacgg tcgcgaccgg caccccggca ggcatctgga cgatcgacaa
     7081 cagggagtcc atcccgtcga gataggccaa cggcaccggc acgccgatga cgggcagcgg
     7141 cgtgaccgac gcgagcatcc ccggcaggtg ggccgctccc ccggccccgg cgatgatgac
     7201 ccgcaggccc cgctcggccg cccccttgcc gtaggcgatc atctcctcgg gcatccggtg
     7261 ggcagacacg acatcgacct cataggcgac cccgaactcg tcgagagcct cggctgccaa
     7321 ggccatcacc ggccagtcgc tgtcgctgcc catgacgatg ccaacgaccg ggtcgtcccc
     7381 gagcgagtca ccggaagacg ttgcagcaga acgacgttca ctcatcgatg actccttgca
     7441 gatagtcggc ggcatgctga gcacgagcgc gggcatcggc caggtcgggc ccgctgacgt
     7501 tgacatggcc aatcttgcga ccgggccgga cccccttgcc atacatgtgc accttcgccc
     7561 ccgggtcacg cgccatgacg tgccggtaag cggggtagat ctcggcatag tcgccgccga
     7621 ggacgttcgc catgaccgtc cacgaggcca ccggccgcgg gtcaccgagc ggcagatcga
     7681 gcacggcccg caggtgttgc tcgaactggc tggtcacggc gccgtcgatg ctccaatgcc
     7741 cactgttgtg ggggcgcatc gcgagctcgt tgacgaggta tgccggtcgc tcgccgaccg
     7801 cggggccgtc cccgtggccg ggcgcggcgc gggcgacctg gaacatctcg acggcgagca
     7861 cccccgtcac gccgagtgcg ccggcgatgc gcagcgcggc gtgggtggcc tcggcggcag
     7921 tcgccggatc cagatccggc gcgggagcga ggacctcggt gcagatgccg tcggtctgga
     7981 cggtctgcac gaccggccag gcggatgcct gcccactggg gctgcgggca acgagcaccg
     8041 ccagctcgcg ttcgaacggc acgagctctt ccaccaggat cccgtcggcg agcgcccctg
     8101 gcgcaccgac ctgctccaac cactcgcctg gcgcatcgag gacgtcgagg tcctccgggc
     8161 cgcgcagcac ccggacgccc ttgccgtcgt atccaccccg gggggtcttg gcgacgatcg
     8221 gccagccgac aaccgcagcg aacgtctcga tgtccgcccg ggtgcgggct tgcgcccagc
     8281 gcgggcaggc cacgccaatc tccgtcagcc gatgacgcat ggccaacttg tcctgagcga
     8341 agcgcagcgc tgccgggctg ggatgcaacg ggaccccgtc ggcttccaga gcggccagca
     8401 cgtcggccgg gacgtgctcg tggtcgaagg tgacgacgtc acaggaggcg gcgaaccggc
     8461 atacggcatc gaggtcggcg tggtcaccca ctggagccga cgggatgacc agggccgcag
     8521 acgcgtcgac cgcttcggcg agaacgctca gctgcacgcc gagcgcgacc gccggggcct
     8581 ggagcatccg cgctagttgc ccaccaccga tgacccccac gacggggaag ccgccgggag
     8641 cgcgccgcgg cgacgtcacc gctccccctg ccgggcgcga ggtgcgccgt cggcgacgtg
     8701 gctcgaaccg accgt
//
LOCUS       DAORPW010000093         8640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1981_length_8640_cov_683.245661, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000093 DAORPW010000000
VERSION     DAORPW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1981_length_8640_cov_683.245661"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="PLB86_08470"
     CDS             complement(<1..176)
                     /locus_tag="PLB86_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRC65209.1"
                     /translation="MPTINQLVRKGRQDKVGKTKTPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVKLTS"
     gene            complement(455..1357)
                     /locus_tag="PLB86_08475"
     CDS             complement(455..1357)
                     /locus_tag="PLB86_08475"
                     /inference="COORDINATES: protein motif:HMM:NF033745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HRC65210.1"
                     /translation="MLLVFVGMGVLVYPVAATQFNNTKQQQFAETYNQRVAQAPTATL
                     AQQLEGAHAYNAALKGVQAKDPWGTLLASGPSSAAYADYLSRLSGLGAMARLRIPSIG
                     VDLPVYHSTSDETIAKGIGHLYGTALPVGGVGTHSVLTSHTGMANATLLDHLIDVREG
                     SAIYVDVAGETLAYQVDQIKVVLPTDIEDLAVDPATDLLTVFTCTPYAVNTHRLLVRG
                     HRIAFDPVVAAAAQPVRPGLVMEPWMYWLIGAAAASFLSLGVALGTAVRARSRRPAPA
                     LGGRASVVRQRRRGNRFGRGIAGR"
     gene            complement(1600..>2220)
                     /locus_tag="PLB86_08480"
     CDS             complement(1600..>2220)
                     /locus_tag="PLB86_08480"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HRC65211.1"
                     /translation="TIHKHLGLPVDQTAYPANGTALVLPTSSAPLAGVVFTVTRVAGV
                     DLTTNAGWYAALAYQDNLEGARAQGGTVHTSTPTGADGSTVVSGLPVGLYLVHEASVR
                     LPDGSEDPTIVKGADFLVTIPMTDPVTRSRWVFDIHVYPKNVRTPTPTPTPTPTPTPT
                     PTKPGTLPWTGSDTASALLVAGLLLGTGAIMAGLGYRRPRERHDGP"
     assembly_gap    2221..2320
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2321..2868)
                     /locus_tag="PLB86_08485"
     CDS             complement(<2321..2868)
                     /locus_tag="PLB86_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HRC65212.1"
                     /translation="MTATLQVQLDPGATGPTQRRRGRHRARRVWVDRLIPVLLMLIGA
                     VVLAYPVVATFYNNYKQSEFAREYAAKVATADPAALNAEFAAATRYNTALNPKLLKDP
                     WDEASSALNADYRDYLSQLDLLEAMGRIRIPALDVDLPVLHGTSDDTLAKGVGHLFGS
                     SLPVGGPSTHAVLTGHSSLANAT"
     gene            complement(3048..4751)
                     /locus_tag="PLB86_08490"
     CDS             complement(3048..4751)
                     /locus_tag="PLB86_08490"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HRC65213.1"
                     /translation="MKSLRMAAAAFAAVALAGLGLIAPTSAMALPSPIIDPTATVTLN
                     IAKFLDNTTGQPNNGTQQTVSGTPLEGVDFDVYKFTTIDLTTNAGWAASIVLQDHTLT
                     AGEIADGFVTVNSVNYPFTLVTTVTTDAAGLATFTQAAGAGLYLVNENLGTSTTTPAA
                     STITPSAPFLVTLPMTDPVNLTSWMYDVYVYPKNQADSITKSVIDGNVGTANQDAYTV
                     GQNLTYKLDSTIQATDTNGSGSITGADLGYYYVGDNLSTYVDAQSVTVSVGGTPLIGC
                     DATAGAGTDCDYYYWLDYAAAAGDEVKIIMSTSGLDKIVAAKVFDAAAVVTTDIVATV
                     TSVPSTGIIPNKGEFIPSEGWWIGQGGTDLTTPGTTTPPTTVPPTTTTPGIPSNEVVS
                     KYGDMQFIKYDAKTSAPLTGAEFTVYRDLNNDDACAPAEMILANQIGSPVTTDASGAV
                     LFTDLQLSNFYNNATQTDLHYYCLVETKAPVGYNLNAQPIPFTVTVEGDVTNLAYTVG
                     TADAQEVQVANEPSNLGNNLPLTGGAGVAAVSIVGLLLVGGGLGYYLVGARRREIEQD
                     V"
     gene            complement(5400..8087)
                     /gene="ppdK"
                     /locus_tag="PLB86_08495"
     CDS             complement(5400..8087)
                     /gene="ppdK"
                     /locus_tag="PLB86_08495"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRC65214.1"
                     /translation="MTTRYVFDFSEGDKEQKALLGGKGANLAEMVKIGLPVPPGFTIT
                     TEACKAYLTKGKAPAELAAEVTAHLATVEQAMGRTLGDPINPLLVSVRSGAAFSMPGM
                     METVLNIGLNDDSVLGLAGMAGERFAWDSYRRLLQMFGKTVLGIDSEVFEARIEQVKH
                     ARRIVEDTELTAADLAALCGSFKTAIFNATGERFPQDPRQQLDAAVEAVFRSWSGDRA
                     VLYRRQERIPNDLGTAVNIQTMVFGNMSDDSGTGVCFTRDPSTGDVGVYGDFLMNAQG
                     EDVVAGIRNARPLHELEGESPAAYRQLLDNMSALEAHYHDLCDIEFTIERGTLWMLQT
                     RVGKRTAEAAFRIAGQLIDEGVISEEEALTRVNGAQLSQLMFPRFDASATSALLTRGV
                     AASPGAAVGKIVFSSIAAKEWADRGEDVILVRTETNPDDLVGMIASKGILTSRGGKTS
                     HAAVVARGMGTTCVCGADELVIDSTHKTITIRGRAGENLKEGDLISIDGGTGQVFRGA
                     VPVMPSAIVEYFEGNIDPEAPETSDTVKAVHRIMTIADKVRRLRVRANADTPEDAARA
                     VRFGAEGIGLCRTEHMFLGDRRELVERLILAETDETRVAALEALRPLQRQDFQEIFAA
                     MGERPVTVRLIDPPLHEFLPNIVEMATKVAIAEERGTATDADRTMLKAIERLHESNPM
                     FGLRGVRLGIVTKGLFEMQVRAILEAFQLARKSGSVGTAEIMVPLIDTYQELELVRTQ
                     ALKIAREKGELVGPGENVMIGTMMELPRACLTAGQIAESADFFSFGTNDLTQTTWGFS
                     RDDVEGSFFSTYLEKGIFPVSPFETIDEDGVGRLVTFAVQQGRETKPGLKTGVCGEHG
                     GDPESVHFFHKVGLDYVSCSPFRVPVARLEAGRAAVMNR"
     gene            complement(8412..>8640)
                     /locus_tag="PLB86_08500"
     CDS             complement(8412..>8640)
                     /locus_tag="PLB86_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65215.1"
                     /translation="DPLRGLKENVILGKLIPAGTGLARYRNIEVEPTEEAKAAMYSMP
                     NYDSYDYSHFGGGTGEAVRLDDLNLDDYGRL"
BASE COUNT         1338 a         2758 c         2983 g         1461 t
ORIGIN      
        1 gaggtcaact tgacgcgggc gaccttgcgc agcgccgagt tgggcttctt gggggtggtc
       61 gtgtagaccc gcgtgcacac gccgcggcgc tggggcgaac ccttgagcgc cggggtcttg
      121 gtcttgccga ccttgtcctg gcgccccttg cgcaccagtt ggttgatcgt aggcaaccta
      181 aactccgtct ttcgatgtgg tcagacccgt ctggcgtgcg gacggactcc ttggtcgttc
      241 cagcttcccc accccggacc cgcgaacggg tcgatcgggc gacgatggcc gggtgccctc
      301 acctgcacgt cccgacgcac tccggcaccc cttgcgaggt gacgtggagg ccgtcgggct
      361 gcccggcgag agcggggcga acgcaggcgt caccggtatg ccggtcgcgc ccaggttctc
      421 gtgtccggag cgagcccaga cacggctaac caatctaccg gcccgcgata ccccgaccaa
      481 atcggttgcc acgtcggcgt tggcggacca cgctcgcccg ccctccgagg gccggcgccg
      541 ggcgacgtga ccgggcgcga accgcagtgc ctagggccac gcccagggag aggaagctcg
      601 ctgcggccgc gccgatgagc cagtacatcc acggctccat gaccaggcct ggccggaccg
      661 gctgggccgc ggcggcgacc acggggtcga aggcgatccg gtgtccgcgc acgagcaggc
      721 ggtgggtgtt gacggcatac ggagtgcagg tgaagacagt gagaaggtca gtcgcggggt
      781 ccaccgcgag gtcctcgata tccgtcggga ggacgacctt gatctggtcg acctgatagg
      841 ccaacgtctc acctgcgacg tcgacgtaga tggccgagcc ctcacggacg tcgatgagat
      901 ggtccagcaa cgtggcattg gccatccccg tgtggctggt gagcacggag tgtgtcccca
      961 caccgcccac gggcagggcg gtgccataca ggtgaccgat gcccttggcg atcgtctcgt
     1021 cgctggtgct gtggtaaacg ggaaggtcga ccccgatcga gggaatccgc agccgggcca
     1081 tcgctccgag cccggacagc cgggacaggt agtcggcata ggccgccgac gacgggccgc
     1141 tggccaggag cgtgccccag gggtctttcg cctgcacacc cttgagcgcg gcgttgtagg
     1201 cgtgggcacc ttcaagttgc tgagcgagcg tcgcggtcgg ggcctgcgcg acccgttggt
     1261 tgtatgtctc ggcgaactgc tgctgcttgg tgttgttgaa ctgggtagct gccacggggt
     1321 agacgagcac gcccataccg acaaagacga ggagcaccga gaccagcgcg cgagccagcc
     1381 ccagcctgcg ccgagcccgg cgctgggcga tcggcccgcc gtgccgagga cgatagccac
     1441 gcgcatcacg caggccccgc tgcggcggac cgggtgcggc atgacgggga cgacgcgacg
     1501 cgtcgggacg ggtgcgcctc gagcgggcag gccgagatcc ccggccggcg ttcctggcgg
     1561 aacgacggct gggaacggcc ccgctcaagg cgtcgtcgct catggtccgt cgtgtcgctc
     1621 tcgtggccgt cggtagccga gcccggccat gatcgccccg gtgccgagca agagcccagc
     1681 gaccaacagc gccgatgccg tgtccgatcc ggtccagggc aacgtccccg gcttcgtcgg
     1741 ggtgggtgta ggcgtgggag tcggggtcgg cgtgggcgtg ggcgtccgga cgttcttggg
     1801 gtagacgtgg atgtcgaaga cccaacgact gcgggtgacc ggatcggtca tcggaatggt
     1861 gaccaggaag tcggcaccct tgacgatcgt cgggtcctcg gagccgtcgg gcagccggac
     1921 gctggcctcg tggacgaggt agaggccgac cgggagcccg gagacaacgg tcgaaccgtc
     1981 ggcccccgtc ggcgtggacg tgtgaaccgt cccgccctgg gcccgggccc cctccaggtt
     2041 gtcctggtag gcgagcgcgg cataccaacc ggcgttggtg gtgaggtcga cgccagcgac
     2101 gcgcgtcacg gtgaagacca ccccggcaag gggcgcagag ctggtgggca gcacgagggc
     2161 ggtgccattg gcagggtatg ccgtctggtc aaccgggagc ccgagatgct tgtggatggt
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtggcgttcg ccaaagaact
     2341 gtgtccggtg agcacggcgt gtgtgctcgg tccgcccacc ggcagcgaac tgccgaacag
     2401 gtggccgaca cccttggcca gcgtgtcatc gctggtgccg tgcaggaccg gaagatcgac
     2461 gtccagcgcc ggaatccgaa tcctgcccat cgcctcgagc agatcgagct ggctcaggta
     2521 gtcgcggtag tccgcgttga gcgcgctgct cgcttcgtcc caggggtcct tcaagagctt
     2581 cgggttcagt gccgtgttgt agcgcgtcgc cgcggcgaac tccgcgttca acgcggccgg
     2641 atcggccgtt gccaccttgg cggcgtactc acgggcgaac tcggactgtt tgtagttgtt
     2701 gtagaacgtt gcgacgaccg ggtaggccag gacgaccgct ccgatcagca tcagaagcac
     2761 cgggatgagc cgatcgaccc acaccctgcg ggcgcgatgc cggccccgac ggcgctgggt
     2821 tggcccagtc gctccggggt cgagctgcac ctgcagggtg gcggtcattc gtctcctcct
     2881 ggatcgggtg gtggggaggg ggttgacgac ctccccacca ccctgatggg tgcggcatgg
     2941 aaggcgaggg ggggttcctt cgcatgccgc tgtcaaacgg gcggcgcttt cacaccgtgg
     3001 ttctcagctc accaatccgg tctgggagga ccgaggttgg agacctgtca gacgtcctgc
     3061 tcgatctcgc ggcgacgggc gccgacgagg tagtagccga ggccaccacc gacgagcagg
     3121 agaccgacga tgctcaccgc agccacgccc gcaccaccgg tgagggggag gttgttgccg
     3181 aggttggacg gctcgttggc cacctgaacc tcctgagcgt cagcggttcc gaccgtgtag
     3241 gccaggttcg tcacgtcacc ctcgaccgtc accgtgaagg ggatcggctg ggcgttgagg
     3301 ttgtagccga cgggcgcctt cgtctcgacc aagcagtagt agtgcaggtc ggtctgggtg
     3361 gcgttgttgt agaagttcga cagctgcagg tcggtgaaga gcacggcacc actcgcgtcg
     3421 gtggtcaccg gcgacccgat ctggttggcc aaaatcatct cggccggggc gcacgcgtcg
     3481 tcgttgttga ggtcacggta gacggtgaac tcagcaccgg tgagcggagc cgacgtcttg
     3541 gcgtcgtact tgatgaactg catgtcgccg tacttcgaga cgacctcgtt cgacgggatg
     3601 ccgggagtcg tggtcgtcgg cggcacggtc gtcggcgggg tcgtcgtccc cggggtggtc
     3661 aggtcggtcc caccctggcc gatccaccag ccctcgctgg ggatgaactc gcccttgttg
     3721 gggatgatgc ccgtcgaggg gaccgaggtg acggtcgcaa cgatgtcggt cgtgacgaca
     3781 gccgccgcgt cgaaaacctt ggccgcgacg atcttgtcca ggccggaggt cgacatgatg
     3841 atcttgacct cgtcgccggc ggcggcggcg tagtcgagcc agtagtagta gtcacagtcg
     3901 gtgcctgccc cggccgtcgc gtcgcaaccg atgagcgggg tgcctccgac ggagacggtc
     3961 accgactgcg cgtccacgta ggtcgacagg ttgtcaccga cgtagtaata gccgaggtcg
     4021 gcacccgtga tggagccgct gccgttggtg tcggtggcct ggatcgtcga gtcaagcttg
     4081 taggtcaggt tctggccgac ggtgtaggcg tcctggtttg ccgtgccgac gttgccgtcg
     4141 atgaccgact tcgtgatcga gtcggcctgg ttcttcgggt agacgtagac gtcgtacatc
     4201 cagctcgtca ggttgaccgg gtcggtcatc ggcagagtga cgaggaacgg cgccgacggg
     4261 gtgatggtcg acgccgcagg ggtcgtggtg gacgtcccga ggttctcgtt gaccaggtag
     4321 aggccagcgc cggcggcctg cgtgaacgtc gccaggcccg ctgcgtcagt ggtcacggtc
     4381 gtgaccaggg tgaacgggta attcacgctg ttgaccgtga cgaaaccgtc ggcgatctca
     4441 cctgcggtga gggtgtggtc ctgcaacacg atcgaggcgg cccaaccggc attggtcgtc
     4501 aggtcgatgg tggtgaactt gtagacatcg aagtcgacac cctccagcgg ggtgcccgac
     4561 accgtctgct gggtgccgtt gttcggctgg cccgtcgtgt tgtcgaggaa cttggcgatg
     4621 ttcagggtga ccgtggcggt cgggtcgatg atcgggcttg gcagcgccat ggccgacgtc
     4681 ggggcgatga gcccgagccc tgccagggcc acggccgcaa aggcagccgc tgccattctg
     4741 agtgatttca tgcgcattcc ttgacactcc tccaaagaga gttttccgtt gttgttctag
     4801 ggagttcgcg gctgcgacct gtcccgtcat cctccgagag cgaccacgtg ggtggcccgg
     4861 ttctggaagt cctcgtccct tcacccgtcc tcgttgagag cccggcggct gcctcacggc
     4921 acccaggaac cctcctcagc ggaggctcat cggctgctcc caaccgatga gacaccaggc
     4981 ggggtcggac gcggacgtcg aagagtggca taccctcggg ggtatgccgt aggcacggca
     5041 gagcaaggct cagcacggct gactccaacg catcgtgttt tgaatcccgc agtcagccgt
     5101 ctcatccggg cgacccctgc gcgtcccccc ggacggcgtc aagactgttc ccgtagatgc
     5161 gcccccacag cgccgaccaa ctgcgtcaat gttaacccag atatacccgc gttgtccagc
     5221 acgacgcgtg cttgctcaag gacgcctcaa gcgcttgcac gtattcacag ccgttcacta
     5281 ttgcttcccc ctagcaaccg gcggccactc gggcctgaag tgcgggagtc atcgtccccg
     5341 atacacagag aacgggcgcg aaccccgcag gggttcacgc ccgttcttgg cgaagtcggt
     5401 cagcgattca tgacggccgc acgcccggcc tcgagacgcg ccaccggcac gcggaacggc
     5461 gagcaggaga cgtagtccag acccaccttg tggaagaagt gcaccgattc ggggtcgccg
     5521 ccgtgctcac cgcagacgcc cgtcttgagc ccaggcttgg tctcccggcc ctgctggacg
     5581 gcgaaggtca ccaaccgccc gacgccatcc tcatcgatgg tctcgaaggg agacaccggg
     5641 aagatgccct tctcgagata ggtcgagaag aacgacccct cgacgtcgtc ccggctgaag
     5701 ccccacgtcg tctgggtgag gtcgttggtc ccgaaggaga agaagtcagc cgactcggcg
     5761 atctgaccgg ccgtcaggca ggcccgcggc aactccatca tcgtgccgat catgacgttc
     5821 tcacccggcc cgaccaactc acccttctcg cgggcgatct tcagcgcctg ggtgcgcacc
     5881 aactcgagct cctggtaggt gtcgatgagc ggcaccatga tctcggccgt cccgaccgaa
     5941 ccactcttgc gggccagttg gaaggcctcg aggatcgccc ggacctgcat ctcgaacagg
     6001 cccttggtga cgatgcccag ccgcaccccg cgcaacccga acatcgggtt ggactcgtgc
     6061 agccgctcga tggccttgag catcgtgcgg tcggcgtcgg tggccgtgcc ccgctcctcg
     6121 gcgatcgcga ccttggtcgc catctcgacg atgttgggca ggaactcgtg caacggcggg
     6181 tcgatgagcc gcacggtgac cggccgctcc cccatcgccg cgaagatctc ctggaagtct
     6241 tgacgctgca gcggacgcag cgcttcaagg gccgccaccc gggtctcgtc ggtctcggcg
     6301 aggatgagtc gctcgaccaa ctcgcgccgg tcgccgagga acatgtgctc ggtgcggcat
     6361 aggccgatgc cttcggcgcc gaagcggacc gcgcgcgctg cgtcttcggg cgtgtcggcg
     6421 ttggcgcgca cccgcagccg gcgcaccttg tcggcgatcg tcatgatgcg gtgcaccgcc
     6481 ttgacggtgt ctgacgtttc gggtgcctcg gggtcgatgt tgccctcgaa gtactcgacg
     6541 atggcgctcg gcatgaccgg cacggcgccg cggaacacct ggccggtgcc cccgtcgatc
     6601 gagatgaggt cgccttcctt gaggttctca ccagcccgac cgcggatggt gatcgtcttg
     6661 tgcgtgctgt cgatcaccaa ctcgtcggcg ccacagacac aggtcgtgcc catgccacga
     6721 gccacaacag cggcgtgcga ggtcttgccg ccccgcgagg tgaggatgcc cttgctggcg
     6781 atcatgccca cgaggtcgtc agggttggtc tcggtgcgga cgaggatgac gtcctcgcca
     6841 cggtcggccc actccttggc ggcgatcgag ctgaagacga tcttgcccac cgccgcgccg
     6901 gggctggccg cgacgcccct ggtgagcagg gccgaggttg ctgacgcgtc gaaccgcggg
     6961 aacatcagct gggacaactg agcgccgttc acccgggtga gcgcctcctc ctcgctgatg
     7021 acgccctcat cgatgagctg gccggcgatc cggaaggccg cctccgcggt gcgcttgccg
     7081 actcgggtct gcaacatcca cagggtgccg cgctcgatgg tgaactcgat gtcgcacagg
     7141 tcgtggtagt gcgcctccag ggcgctcatg ttgtccagca attgccggta tgccgcgggc
     7201 gactcgccct cgagctcgtg cagcgggcgg gcgttgcgga tgccggcgac gacgtcctcg
     7261 ccctgggcgt tcatgaggaa gtcgccgtag acgcccacgt cgcccgtgga ggggtcgcgg
     7321 gtgaagcaga ccccggtgcc cgagtcgtcg gacatgttgc cgaagaccat cgtctggatg
     7381 ttgacggccg tgccgaggtc gttcggaatg cgctcctggc gacggtagag gacggcgcgg
     7441 tcaccactcc aggaccggaa gacggcctcg accgccgcgt cgagctgctg gcgcgggtcc
     7501 tgcgggaagc gctcccccgt ggcgttgaag atcgccgtct tgaagctgcc acacagcgcg
     7561 gccaggtcgg cggcggtcaa ctcggtgtcc tcgacgatcc ggcgggcgtg cttgacctgc
     7621 tcgatgcgcg cctcgaagac ctcggagtcg atccccagga ccgtcttgcc gaacatctgc
     7681 agcagtcggc ggtaggagtc ccaggcgaac cgctcgccgg ccattccggc cagcccgagg
     7741 accgagtcgt cgttgagccc gatgttgaga acggtctcca tcatgccggg catcgagaac
     7801 gctgcgccgg aacgcaccga caccagcagg gggttgatcg ggtcgcccag agtgcgtccc
     7861 atcgcctgct ccaccgtggc gaggtgcgcg gtgacctcgg ccgccaactc cgccggcgcc
     7921 ttgcccttgg tgaggtaggc cttgcaggct tcggtcgtga tggtgaaacc ggggggcacc
     7981 ggcagcccga tcttcaccat ctcggcaagg ttggccccct tcccgcccag gagcgccttc
     8041 tgttccttat caccctcgga gaagtcgaag acgtagcggg tggtcatggg tcgtccttcc
     8101 ggggaaaagg ggcggggggt gccgcagtgc actcacccca tcttggaccc atttgggcct
     8161 tccgaagcgg ctctccgggg gacgaaggtc ccgcagactt gacggcaccg tggagtcgcg
     8221 aacgcgcttg cgcggcaccg ggttggagcc gcgacgctcc ctgatcgtca attccctgac
     8281 tagataagga ctttcgcgtc gaggggcctc cacaacacgg tatgccgccc cccacaacgc
     8341 ggtatgccgc ccccgcaggt gcggggacgg cataccgagg actgcgccgg tgacccggca
     8401 gcgactgggg cctacaggcg cccgtagtcg tcgaggttga ggtcgtccag acggaccgcc
     8461 tcgcccgtgc ccccaccgaa gtgggagtag tcgtaggagt cgtagttggg catggagtac
     8521 atggccgcct tcgcctcctc ggtcggctcg acctcgatgt tgcggtagcg ggccaggccc
     8581 gtgccggcgg ggatgagctt gccgaggatg acgttctcct tgaggccgcg cagcgggtcg
//
LOCUS       DAORPW010000094         8601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_1988_length_8601_cov_676.667915, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000094 DAORPW010000000
VERSION     DAORPW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8601
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1988_length_8601_cov_676.667915"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..601)
                     /locus_tag="PLB86_08505"
     CDS             complement(2..601)
                     /locus_tag="PLB86_08505"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC65216.1"
                     /translation="MLALAGRSDWSGATVLDIGCGAGFHLPRLAESAEAVIGVEPHAP
                     LVALAQDRTAGLDNVSVRLASAQATGLPSESVDVATARWAYFFGPGCEPGLVELTRIM
                     RPGGLAFFVDVDATRSTFGAWFRGSLPDYDPAAVERFWRRQGWVQERLEVAWEVSTRS
                     DFEAVVRLEFAPTYAGRILAEDPDRTGVDYAVNLWWRRF"
     gene            complement(755..1552)
                     /locus_tag="PLB86_08510"
                     /pseudo
     CDS             complement(755..1552)
                     /locus_tag="PLB86_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1608..2552
                     /locus_tag="PLB86_08515"
     CDS             1608..2552
                     /locus_tag="PLB86_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRC65217.1"
                     /translation="MRLGVIDVGSNTVHFLVVDAYYGGHPLPAYSHKVELKLSENLLP
                     DGTIGAEFGRALTQFLTECQEIAEEQGVIDLMAFATSAIRDAQGGQELIETVSRESGV
                     DLQVLSGRDEARLTYLAVRRWFGWSSGRLLVVDIGGGSLELASGLDEEPDAAISLPLG
                     AGRLTRDLLADDLPKPKDIKAARAYVRAEIGGAVRELLRFGPPTRAVGTSKTIRSLAR
                     IAGAAPSGEGIYVTRRLRREHLGDIVKRLGRMSVAERAELPGVSGTRAHQLLAGALVV
                     EGAMDLLRLDRLDIAPWALREGIILRRLDHLEADGPLS"
     gene            2594..3370
                     /locus_tag="PLB86_08520"
     CDS             2594..3370
                     /locus_tag="PLB86_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRC65218.1"
                     /translation="MHIRVSLSTASVYPGTCATAFELASRLGYDGIEVMVWTDVVSQE
                     AGALRALSRHYGIPVLSVHAPTLLITQRVWGPEPWTKIDRSIELAADLDAPTVVLHPP
                     FRWQKEYAAEFVDGVAAREAESGVRLAVENMFPWRARGRDLQAYLPHWDPVPMSYDHV
                     TLDLSHTATAGSDALAMAADLGERLTHVHLADGSGSVRDEHLVPGRGGQPCAEFLQLL
                     PGSGFEGDVVVEVGTRKHTDLQRAEDLRESLDFARAHLGE"
     gene            complement(3429..4241)
                     /locus_tag="PLB86_08525"
     CDS             complement(3429..4241)
                     /locus_tag="PLB86_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HRC65219.1"
                     /translation="MPASLSFAAYGEGIGNASAVVRANAVSAGLTAPVPSCPGWTVLD
                     LVLHLGTAHRWASAIIEGVPQDRWPTEKSGRAEGLASGDVLEWFDDGMAHLLHVLASA
                     PADLKAFFFLKDAGRPREAWARRQAHETTIHGVDAMAARLGRVPTASQTWVTAALAAD
                     GVDELLTGFVPRKSGKLRTAPGEPPLTVLVRATDIDRAWTLRVSADPVITTTGTRAQE
                     EAHGIPDVVWEGTAAALYLGLWNRGDEITEIGETGERSFLDRWRTDQRVQWT"
     gene            4367..5314
                     /locus_tag="PLB86_08530"
     CDS             4367..5314
                     /locus_tag="PLB86_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRC65220.1"
                     /translation="MEASDLLRTSLLQLAKMHRVRDVVERAPVSRAVVRRFVAGSAVA
                     DAVRVAGELAAVGRSSTIDYLGEDTTQLDQARHTRDTYLTLLRELSARGLSHGGKAEV
                     SVKLTALGLHLPDGPAVALGHAREIAQAAVNAGTTLTVDMEDHTTTDSTLRIVAQLRE
                     DFPSVGAVLQAYLHRTEADCRELANAGSRIRLCKGAYQEPETVAYQDGAEVDRAYVRC
                     LKILMAGDGYPMVASHDPRLVEIAAALAGRAGREPKTFEYQMLYGIRPDEQQRIADRG
                     DQMRVYIPFGEEWYGYLMRRMAERPANVAFFLRGVATKG"
     gene            5441..6247
                     /gene="proC"
                     /locus_tag="PLB86_08535"
     CDS             5441..6247
                     /gene="proC"
                     /locus_tag="PLB86_08535"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRC65221.1"
                     /translation="MATTAIFGAGVMGETLLSGLLRSGRPADDIVITEKRADHAATLR
                     ERYGVRVLDNAQAAEVAEVLVLVVKPQDMDGLLGEIREHIAPGNLVVSLAAGIPTAYL
                     ESRLPEGSSVVRVMPNTPALVDQGMAAISPGRNCTADHLAEAEALLASCGKVLQIAEK
                     HQDAVTAISGSGPAYIFYVVESMIEAGVLLGLPRATSTELVVQTLYGAATMLRETGEH
                     PTVLRERVSSPGGTTMAAIRQLEDHKVRAAFMTAMEAAAARSAELAAGLT"
     gene            6244..6828
                     /locus_tag="PLB86_08540"
     CDS             6244..6828
                     /locus_tag="PLB86_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC65222.1"
                     /translation="MTDAVSAGLPVGLSAALSMVRLTPDDWEVHRDLRLEALATDPEA
                     FGATYADNAAYDEATWRARLAAVTYWQVREAGMPLGMVGLWDPLADGVADEAGVQGDA
                     GEGADDERVPFVIGMFVRAAARGRGVGSALIDQAIAEAGERGCARLALDVHAGNTAAR
                     ALYERHGFQVFADAVLSEDRRASGCELTMVRRLR"
     gene            6884..7414
                     /locus_tag="PLB86_08545"
     CDS             6884..7414
                     /locus_tag="PLB86_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC65223.1"
                     /translation="MSESIDPITVRRLKDADWPEFRRVRLAALGESPEAFSATLAEEE
                     ASDEASWLERVQRSPRFLAESGGAVVGMASLGRYQGAEGESENSGELYGLWVTPALRG
                     TGVATALVNAASRQASDDGRSHVVYWVSTSNGRAVAFASGLGFRPTDDRRPMRGVGHA
                     DEEEIAMTLPLGLDRG"
     gene            7559..>8601
                     /locus_tag="PLB86_08550"
     CDS             7559..>8601
                     /locus_tag="PLB86_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HRC65224.1"
                     /translation="MPSQARVVWDPDFTKYNFGPSHPMQPVRLALTARLCEEFGIFTD
                     GDVEVLAPDVVDDAWLQTVHEPYFIDAVKAASNDPEHTNEHLGIGTDDVPTFAGMHEV
                     SARIVEGTRAVCEAVWTGETAHGVNFCGGLHHAMAANASGFCVYNDIAVGIQWLLDHG
                     ADRVAYVDVDVHHGDGVESIFWNDPRVLTVSIHETGRALFPGTGWATDIGGEAALGHA
                     VNVSLPPGLSDSGWLRALHSVAMPVVRAFRPQVLVTQHGADTHFQDPLAHFALSLDAQ
                     REAMVALHRLSHEVCGGKWVALGGGGYEVIDVVPRAWTHLTAIAAHRPILPGTAVPQA
                     WRDYVESLFGRPGP"
BASE COUNT         1252 a         2967 c         3090 g         1292 t
ORIGIN      
        1 gtcagaagcg gcgccaccac aggttgacgg cgtagtccac cccggtccgg tcgggatcct
       61 cggccagaat ccgaccggca tacgtggggg cgaactccaa ccgcacgacc gcctcgaagt
      121 ctgagcgcgt gctcacttcc cacgcgacct cgagccgttc ctgcacccag ccctgccgtc
      181 gccagaaccg ctcgacggca gccgggtcgt agtcgggcag cgagccgcgg aaccaggcgc
      241 cgaacgtcga tcgcgtcgcg tccacgtcca cgaagaacgc gaggccgccg gggcgcatga
      301 tccgggtcag ctcgaccagg cctggctcgc accccggccc gaagaagtag gcccaccgag
      361 cagtcgcgac gtcgacggac tcgctcggca acccggtggc ctgggccgag gccagtcgca
      421 cgctgacgtt gtccaggcca gcggtgcggt cctgtgccag cgcgaccagc ggcgcgtgcg
      481 gctccacccc gatgactgcc tcggccgact cggcgagccg cggcaggtga aagccggccc
      541 cgcagccgat gtcgagcacc gtcgccccgg accagtcgga gcggccggcg agggccagca
      601 tggtcgcgtc gatgaggccg gcccggtcaa gggcgcggtt ctcgagctcg tagatctcgg
      661 gggagttcca gatgttagga ctgcgaatgg cgccgtcggc gtccatcgtg ttgcccttga
      721 ccgtcaacgg tttgcgacgc agccgccgcc gggactcggc catcaacgtg gccgggtgca
      781 ccagcaaggg caacagctcg cgccccgacc cccgccgcgc cgagttgacg ctgcgcaggt
      841 atgccgtgca cctcaccaca agttggtcgt aggcggcctc gcccatgagc tcgacgagct
      901 cgtcgcggtt ggaccggaac accggttcga ggccgacgtg ggcgtccgag ctgccggagc
      961 agaaccagtc gaggtcgtgc ccccccgggc cccacccacg acgatcgaac tcggtgatcg
     1021 aaatctcgag atagctcgtg ccgtcctgca gctccaccgt gcggtaggac cggcgaatgg
     1081 gcagctgcca gcagacctcg ggcttgacct cgtgaggttt ggtcccgtcc ttgacggcgc
     1141 gctggtgcag ggcacagccg ggaccggccg cgaagccggg ccggttgagg aagatgcagg
     1201 cgccctcgac gaccttggtc ttgcgggcgt cgtcctcccg ctcggtccag ttgcgctgcg
     1261 acagtgaccc accggcatac gcgaggtgtc ggaactgcca ctcgtcctcg gtgagggcgc
     1321 tcgcagcgtc gcgcacccgc tggtagtcgg ccttctccgt gaagtgggcc ccgagggtgc
     1381 agcagccgtc gtcgggtcgg ttggcgtaga ggcctcggca cgccggtgtc ccgaagacgc
     1441 acgtccagcg ggaggtgagc cacgtgaggt cgcaccggaa gcgttggcgc gggttgtcag
     1501 gatcggtgaa ctcgacccac gttcgggcca ggtccagtgg agcttcaggc acggcgcaca
     1561 ctctaaccgc ggtcgtcgcg gtccgtggcg ctcggtaccg ttcgggtatg cgcctcggag
     1621 tgatcgacgt cggttccaat accgtccact tcctcgtggt cgacgcctac tacggaggtc
     1681 atccgctccc ggcctactcg cacaaggtcg agctcaaact cagcgagaac ctcctgccgg
     1741 acgggacgat cggggccgag ttcggcaggg cactgaccca gttccttacc gagtgccagg
     1801 agatcgccga ggagcagggc gtcatcgacc tcatggcctt tgccacctcg gcgatccgcg
     1861 acgcccaagg cggtcaggag ctgatcgaga ccgtctcgcg ggagtcgggt gtcgatctgc
     1921 aggtgctcag cggtcgggac gaggcccgcc tcacctatct cgcggtgcgt cggtggttcg
     1981 ggtggtccag cggccggctg ctcgtcgtcg acatcggtgg cggatcgctc gagctcgcct
     2041 cgggtctgga cgaggagcct gatgcggcga tcagcctgcc gctgggcgcg gggcggctca
     2101 cccgggacct cctggccgac gacctgccca aacccaagga catcaaggca gcccgggcct
     2161 acgtgcgcgc cgagatcggc ggcgcggtcc gtgagttgct gcgcttcggg ccacccaccc
     2221 gcgccgtcgg cacgtccaag acgatccgct cgctggcccg gatcgccggc gcggcgccga
     2281 gcggtgaggg catctacgtc acccggcggc tgcggcgcga acacctcggc gacatcgtca
     2341 agcggctcgg tcggatgtcg gtcgccgaac gcgcagaatt gcccggcgtc tcgggcactc
     2401 gtgcccatca gttgctcgcc ggcgcgctcg tcgtcgaggg cgccatggac ctgctgcgcc
     2461 tggaccgact ggacattgct ccctgggcgc tccgggaagg catcatcctg cggcgcctgg
     2521 atcacctgga agctgacggg ccgctgagct gacccggcgc acgcgggcca gcgggaccgt
     2581 tcacaatgag ggcatgcaca tccgggtgag cctgtcgacc gcttcggtct acccgggcac
     2641 gtgcgcgacg gcgttcgagt tggcctcccg gctgggttat gacggcatcg aggtcatggt
     2701 ctggaccgac gtcgtctccc aggaggccgg tgccctgcgc gccctgtccc ggcactacgg
     2761 catacccgtg ctgtcggtcc atgcacccac gctgctcatc acgcaacgcg tctgggggcc
     2821 ggagccctgg accaagatcg accggtcgat cgagttggcc gccgacctgg acgcaccgac
     2881 ggtcgtgctg cacccgccgt tccgttggca gaaggagtat gccgcggagt tcgtcgacgg
     2941 ggtcgctgca cgcgaggccg agtcgggggt gcgcctggcg gtggagaaca tgttcccctg
     3001 gcgggcccgg ggccgcgacc tgcaggccta cctcccgcat tgggacccgg tgccgatgag
     3061 ctacgaccac gtcacgctcg acctctcgca cacggcgacc gccggctcgg acgccctggc
     3121 gatggccgcc gacctggggg agcgcctgac tcacgtccac ctggccgacg gctcggggtc
     3181 ggtgcgcgac gagcacctcg ttccgggtcg gggcgggcag ccgtgcgcgg agttcctgca
     3241 gttgctcccc ggctcggggt tcgagggcga tgtcgtcgtc gaggtgggga cccgcaagca
     3301 caccgacctc cagcgcgccg aagacttgcg cgagtcgctc gacttcgcgc gggctcacct
     3361 gggggagtaa acgactggcg gtgcgccgac ggaccgccgg accgtcgggc cgtcagcggc
     3421 tcagccggtc acgtccactg gacccgttgg tcggtgcgcc accggtcaag gaaggaccgc
     3481 tcgcccgtct cgccgatctc ggtgatctcg tcaccgcggt tccacaaccc gaggtagagc
     3541 gccgccgcag tgccttccca cacgacatcc ggtatgccgt gcgcctcctc ctgcgcgcgg
     3601 gttccggtcg tggtgatgac cgggtcggca gacacccgca acgtccaggc ccggtcgatg
     3661 tcggtggctc ggaccaacac cgtcaacggc ggctcgccgg gagcggtgcg gagcttgccg
     3721 gacttgcgcg ggacgaaccc ggtgagcaac tcgtcgaccc cgtcggcggc caacgctgcc
     3781 gtcacccagg tctggctggc ggtgggaacc cggcccaggc gggcagccat ggcatccacg
     3841 ccgtggatcg tcgtctcgtg cgcctgccgc ctggcccagg cctcgcgtgg ccgtccggcg
     3901 tccttgagga agaagaacgc cttgaggtct gcgggtgcgc tggccaggac gtgcaggagg
     3961 tgggccatgc cgtcgtcgaa ccactccagg acgtcgccgc tggccaaacc ctccgcgcgg
     4021 cccgacttct cggtcggcca gcggtcctgc ggcacgccct cgatgatggc gctcgcccac
     4081 cggtgggccg tgccgaggtg caggaccagg tcgaggacgg tccacccggg gcaggacggg
     4141 acgggcgcag tgaggccggc gctcacggcg ttggctcgca cgacggccga ggcgttgccg
     4201 atcccctccc cgtaggcggc gaaggacagg ctcgcaggca tgaagcaact gtacgagacc
     4261 gacccagcgc cgtcacggcc gcgcgtcgcc accgcgcgtt gccagcgtgg gttccgcgag
     4321 cgcttcgaca ccccaccacc cccgcggcgg tcgtagagtg gtcgccgtgg aagccagtga
     4381 cctcctgcgc acttctctcc tgcagctggc caagatgcac cgggtgcgcg acgtcgtcga
     4441 gcgggcgccg gtcagccgag cggtcgtgcg acgcttcgtc gccggatcgg cggtggccga
     4501 cgcggtccgg gtggccggcg agctcgccgc ggtcggccgg tcgagcacca tcgactacct
     4561 cggcgaggac accacccaac tggaccaggc gcggcatacc cgcgacacct atctcaccct
     4621 cctgcgcgag ttgtccgcgc ggggtctgag ccacggtggc aaggccgagg tgtcggtgaa
     4681 gttgaccgcc ttgggcttgc acctgcccga cggccccgcg gtcgcgctgg gccacgcgag
     4741 ggagatcgcc caagcggcgg tcaatgccgg gacgacgctg accgtcgaca tggaagacca
     4801 cacgacgacc gactcgacgc tgcgcatcgt cgcgcaactg cgtgaggact tcccctccgt
     4861 gggggccgtg ctgcaggcct acctgcaccg caccgaggcc gactgccgcg agttggcgaa
     4921 cgcgggctcg cggatccggt tgtgcaaggg ggcctaccag gagccggaaa cggtcgccta
     4981 ccaggacggg gccgaggtcg accgggccta cgtgcgctgc ctgaagatcc tcatggccgg
     5041 cgacggatac cccatggtgg ccagccacga tcctcggctc gtcgagatcg ccgcggcact
     5101 ggctggccgg gccgggcggg agcccaagac cttcgagtac cagatgctct acggcatccg
     5161 ccccgacgag cagcagcgca tcgccgaccg cggcgaccag atgcgcgtct acatcccctt
     5221 cggcgaggag tggtacggct acctcatgcg ccggatggcc gagcgccccg ccaacgtcgc
     5281 gttcttcctt cgcggggtgg ccaccaaggg ctgagggtat gccgcgcgcc gataaagctc
     5341 ggcaggcaga ctctcccggt cggtaggctc gcggcgccga cgtgcggcgg cagaatcgtt
     5401 gcgcgggaac tcggcgagac ctcgacggaa gtgacacatc atggcgacga cggcgatctt
     5461 cggagcgggc gtgatggggg agaccctgct gtccgggctg ctgcgctcgg ggcgtccggc
     5521 cgatgacatc gtcatcaccg agaagcgggc cgaccatgcc gcaaccttgc gggagcgcta
     5581 cggcgtgcgg gtcctggaca acgcgcaggc ggccgaggtg gccgaggtcc tcgtgctcgt
     5641 cgtcaagccg caggacatgg acggcctgct cggggagatc cgtgagcaca tcgcgccggg
     5701 caacctggtc gtgtccctgg cagccggcat ccccacggca tacctcgaga gtcggctgcc
     5761 cgagggctcc tcggtcgttc gggtcatgcc caacacgccg gccctcgtcg accaggggat
     5821 ggcggcgatc tccccgggac gcaactgcac cgcggaccac ctcgccgagg cggaggcact
     5881 gctggcctcc tgcggcaagg tcctgcagat cgcggagaag caccaggatg ccgtcacggc
     5941 gatctccggc agcggaccgg catacatctt ctatgtcgtc gagtcgatga tcgaggcggg
     6001 ggtgctgctg ggtctgccgc gcgccacctc gaccgagctc gtcgtgcaga ccctttacgg
     6061 ggcggcgacg atgctgcgtg aaacgggcga gcacccgacc gtcctgcgcg agcgggtgtc
     6121 cagccccggc ggcacgacga tggcggcgat ccgccagctc gaggaccaca aggtgcgggc
     6181 ggccttcatg accgccatgg aggcggccgc ggcgcgctcg gcggagctcg ctgccggact
     6241 gacatgacgg acgcggtgtc ggcgggtctc ccggtgggtc tgtcggcggc gctctcgatg
     6301 gtccggctga cccccgatga ctgggaggtc catcgggatc tgcgcctgga agcgctggcg
     6361 accgaccccg aagccttcgg cgcgacctac gccgacaacg cggcatacga cgaggcgacc
     6421 tggcgcgcgc ggttggcggc ggtgacctat tggcaggtcc gcgaagcggg tatgccgttg
     6481 ggcatggtcg gtctgtggga cccgctcgcc gatggtgtcg ccgacgaggc tggggtgcag
     6541 ggtgatgcgg gggagggtgc tgatgacgag cgagtgccgt tcgtcatcgg gatgttcgtc
     6601 cgcgccgcgg cgcgtgggcg cggtgtgggc tcggccctca tcgaccaggc gatcgccgag
     6661 gcgggtgagc gcggctgtgc gcgcctggcc ctcgacgtcc acgccggcaa caccgcggcc
     6721 cgcgccctct atgaacggca cggcttccag gtgttcgctg atgccgtgct gagcgaggat
     6781 cgccgggcga gcggctgcga gctgaccatg gtgcgccgcc tgcgatgaga gcgcccgcgg
     6841 acaaccccct gcgcagccgt cccgccgtga aagggtgagc cccatgagcg agagcatcga
     6901 tccgatcacc gtgcgccggc tgaaggacgc cgactggccg gaattccgac gggtgcgtct
     6961 ggccgccctc ggtgagtcgc ccgaggcatt ctcggcgact ctggcggagg aggaggcctc
     7021 ggacgaggcg tcctggctgg agcgggtgca gcggtcccct cgcttcctcg ccgagtccgg
     7081 cggcgccgtc gtgggcatgg ccagcctcgg ccgctaccaa ggcgcggagg gcgaatcgga
     7141 gaattcgggc gagctctacg ggctgtgggt cacgcccgcg ttgcgtggca cgggggtggc
     7201 gacagccctg gtcaacgccg ccagccgtca ggcaagcgac gacggacggt cacacgtcgt
     7261 ctattgggtg agcacgtcga acggccgagc ggtcgccttc gcgtcgggct tgggcttccg
     7321 gcccaccgac gaccgccgtc cgatgcgtgg cgttggccac gctgacgagg aggagatcgc
     7381 catgaccttg ccgctggggt tggaccgcgg ttgaccgatc gctcgggtcc gcgtgcgcgc
     7441 gcgaccacct ggaagaacgg cataccggat cgcgttccgc tgcgcggcat cggtgcccgt
     7501 gagaggtctg tgtcggccgt cgtcgtgacc gcaacggaga aggggtagcg tcgtccttat
     7561 gcctagccag gcccgggtcg tctgggaccc ggatttcacg aagtacaact tcgggccctc
     7621 gcaccccatg cagccggtcc gcctggccct gaccgctcgg ctctgtgagg agttcggcat
     7681 cttcaccgac ggcgacgtcg aggtgctggc tcctgacgtc gtcgacgacg cctggctgca
     7741 gacggtgcac gagccctact tcatcgatgc ggtgaaggcc gcctcgaacg accccgagca
     7801 caccaacgag cacctgggca tcggcaccga cgacgtgccg acgttcgcag ggatgcacga
     7861 ggtgagcgcg cgcatcgtcg agggcactcg ggccgtgtgc gaggccgtct ggaccggtga
     7921 gaccgcccac ggcgtcaact tctgcggcgg cctgcatcac gcgatggcgg ccaacgcctc
     7981 aggcttctgt gtctacaacg acatcgcggt cggcatccag tggctgctgg accatggggc
     8041 cgaccgggtg gcttacgtcg acgtcgacgt ccaccacggc gacggcgtcg agtcgatctt
     8101 ctggaacgac ccccgggtgc tcaccgtgtc gatccacgag acggggcggg cgctcttccc
     8161 tggcaccggg tgggcgaccg acatcggcgg cgaggcggcc ctgggccatg cggtcaacgt
     8221 gtcgctgccg ccagggctgt ccgactccgg atggctgcgc gcgttgcact cggtggcgat
     8281 gccggtggtt cgggccttcc gcccgcaggt gctcgtcacc cagcacgggg cggacacgca
     8341 cttccaggac ccgttggccc acttcgccct ctcgctggat gcgcagcggg aagcgatggt
     8401 cgccctgcac agactcagcc acgaggtgtg cggtgggaag tgggtggcgt tgggtggcgg
     8461 cggatacgag gtcatcgacg tcgtcccgcg cgcctggacg cacctgacgg cgatcgccgc
     8521 gcatcgcccg atccttccgg gaaccgccgt gccgcaggcc tggcgggact acgtcgagtc
     8581 gttgttcggt cgtccggggc c
//
LOCUS       DAORPW010000095         8548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2006_length_8548_cov_852.437890, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000095 DAORPW010000000
VERSION     DAORPW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2006_length_8548_cov_852.437890"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1102)
                     /locus_tag="PLB86_08555"
     CDS             complement(62..1102)
                     /locus_tag="PLB86_08555"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC65225.1"
                     /translation="MRIAVAGAGLIGRRHIEELVKSPHASLASIIDPSPEAGALAAEL
                     GVPHYVTLERALADDRPDGVILATPNRLHVEGGLTCIAAGVPVIVEKPLAETAAGAKQ
                     LVEAAEAAHVPLLTGHHRNYSANMAAAREVISSGVLGRLVAIVGQATFYKPDDYFDVG
                     DGWRRKAGGGPILLNLTHEVNNLMALAGDITAVQATTSSATRGFEVEDTAAMIFHFAG
                     GVLGTFMLSDTAASPRSWEQTSGENTSYATYPDEDSYHVMGTVGSLSIPTMRLKTYPG
                     VQSWWSATDDSRIDVAVEDPLANQIAHFAAVIRGEAEPICDGRAGLKTIQVCDAVQEA
                     ARTGAVVNTGIS"
     gene            complement(1231..2538)
                     /gene="mshA"
                     /locus_tag="PLB86_08560"
     CDS             complement(1231..2538)
                     /gene="mshA"
                     /locus_tag="PLB86_08560"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HRC65226.1"
                     /translation="MTPEEIRRVAMFSVHTSPLEQPGTGDAGGMNVYIAEVAKELARQ
                     NREVEIFTRATTGALPPTVELVPGVTVRHITAGPYEGLAKNDLPGQLCAFAAGVMRAG
                     AARPEGWYDLVHSHYWLSGQVGWLAADRWRVPLVHTMHTMARVKNAALADGERPEPAG
                     REIGEAQVVEAADLLTANTADEACQLVSLYDADPARVVVVPPGVDLKVFTPGDRYAAR
                     ARLGLPQGAHVVLFVGRIQPLKAPDLLVKAARRLLDAHPDWTPDDLVVGILGGPSGTG
                     LEHPESLATLAAELGMTAYVRFVPPADRATLAQWYRASDLVTVPSYNESFGLVAIESL
                     ACGTPVVAARVGGLPTAVGPGGVLVDGHDVDDWAATLDRMLADPGLRAELGRRAVRHA
                     RSFGWGATTERLLQAYGEACRTRTPSPIFEASLLTGVPAAVVP"
     gene            2653..3465
                     /locus_tag="PLB86_08565"
     CDS             2653..3465
                     /locus_tag="PLB86_08565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC65227.1"
                     /translation="MNRTRAEGGPPLGRITRGTTNPNRLRRCDRWLAGTQAWRLRAPM
                     DPPVVVDLGYGASPVTAVELFERLRRVRGDVEVLGLEIDPERVAAATGLERPGLSFRR
                     GGFEVPTADGRAVTVVRAFNVLRQYEEDEVEAIWAKVRSRLAPDGVLIDGTCDELGRL
                     ASWIAVEPAAGPVSLTLSMRLRELSTPADVAERLPKALIHRNVPGERVHGFLVALDQA
                     WRYAAPQAAYGVRARFLETAARMRANGWPVLDGPARWRLGELSIRWDAVRPG"
     gene            complement(3484..>3903)
                     /locus_tag="PLB86_08570"
     CDS             complement(3484..>3903)
                     /locus_tag="PLB86_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HRC65228.1"
                     /translation="HHRTEYVEGLRDPRVDIFVANRPWGSFTQFVSNETVTVKTILVE
                     AGHRLSLQRHEHRGEMWTMLDVPIDVTVDDRTWTAGVGETVWVPRGATHRIANPGEKA
                     GRILEVGFGFFDESDIERLEDDYARHDDAEGSDHAEA"
     assembly_gap    3904..4003
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4103..4555)
                     /gene="dtd"
                     /locus_tag="PLB86_08575"
     CDS             complement(4103..4555)
                     /gene="dtd"
                     /locus_tag="PLB86_08575"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRC65229.1"
                     /translation="MRVVLQRVSAARVEVDGVVVGDLDGPGLVALVGVTHGDGPTQVE
                     WMARKIAHLRVFDGPDGDESPGAERSAVEAGLPVLVVSQFTLYGDVRKGRRPSWSAAA
                     PGPVAEPLVEAVAEALRAEGLRVATGTFGAHMRVHLVNDGPVTLVLDV"
     gene            complement(4563..5570)
                     /locus_tag="PLB86_08580"
     CDS             complement(4563..5570)
                     /locus_tag="PLB86_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRC65230.1"
                     /translation="MSNPSSHAAAHPVSPALTQGPVLAHVVRCGVVESVHRGSAVVTS
                     ADGSIEQAWGDPTGLIFPRSASKPIQAAAMVAAGLDIAPHQLALVAASHSGEAFHLAA
                     ALEILHGAGLDETHLLNTPDYPVDEQARDEWIRAGHPKTSLAQNCSGKHSGMLATTAA
                     NGWDLATYLDPEHPLQQAITAAVARLSGTAVGAIAVDGCGAPLHGIPLTGLAHAFGRI
                     AAAPAGSPEARVADAIRAHPEYLGGTRRGVTTLIRETPADQRLIAKDGAESVYAVGLG
                     DGRGVAVKIADGHMRAKPMVLAAILRRIGVGSEAGYAALEDAPVLGHGQPVGSIVAVG
                     I"
     gene            complement(5754..6527)
                     /locus_tag="PLB86_08585"
     CDS             complement(5754..6527)
                     /locus_tag="PLB86_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65231.1"
                     /translation="MARKTRMDEMKKDLQKTATEARTQLMAVVGATDLAVEKIRVAQE
                     HFVQVAREMDAKSLQKDAERFQKDVKKVQKNLAKIQADLTKRLSKTAKDVQTVPANAV
                     GHSIELATKAQATVDELAERGEALVQRIRTTRSTQDLKSQVETTVAAGKGAVTTARKA
                     AVSTERAAKATIKSAAEEAKVVAAKVAETVEKDAETVASTVKASAARTRAAARTTTTT
                     AQAGVKRTTARTRATGTGVRKTAAKTTTAAKTAAKKVGD"
     gene            complement(6543..6980)
                     /locus_tag="PLB86_08590"
     CDS             complement(6543..6980)
                     /locus_tag="PLB86_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC65232.1"
                     /translation="MTPTLKLDLGSYLREQRRAAKLSLRQLSELAGISNPYLSQIERG
                     LKKPSAEILQQIARGLEVSAESLYVRAGILDESSPTSAPSVLAAIAADGRLTDRQRQV
                     LVDVYRSFVDIADEPRTAPATGSASDGDVAPAPAEQSTETPES"
     gene            7057..>8548
                     /locus_tag="PLB86_08595"
     CDS             7057..>8548
                     /locus_tag="PLB86_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HRC65233.1"
                     /translation="MAGLLVAGTSSDAGKSLIVAGLCRAWARQGVRVAPFKAQNMSNN
                     SMVCADGSEIGRAQHLQAIAAGVEPSALHNPVLLKPSTDRRAFIVLRGQPGGTLEAGE
                     YVGGRTRLADAAYEAYAVLAGTHELVVCEGAGSPAEINLRAGDYVNFGLARRFGLPTL
                     LVGDIDRGGVLASIFGHWGIVDDSDRALLAGYLINKFRGDQSVLDPGLDELTRRTGLR
                     CFGVVPWLDQVWLDSEDALAFAKWPRLPGRAGTLRVAAIRLPRISNATDLDAIASEPG
                     VDVFATAEPADIEHADLVVLPGSRATVADLEWLRRLGLADALVRRAAEGRPILGICGG
                     YQMLTAEIDDPVESPSGGAAGLGLLPARVRFQPEKVLGRPRGRWRGHDVVAYEIHHGV
                     VEPLAGSGGEGGLGGERSERGAGSEPFLDGIRDGAVWGTIWHGAFENDGFRRAWLAEI
                     AAAVGSTWVPRADAPGFSERRERMLDDLADALEACVDLPALLAAARG"
BASE COUNT         1178 a         2764 c         3177 g         1329 t
ORIGIN      
        1 caagtcgtgt tcttgggcca cgatcacaac ccgcgcgctg gatcgaccgt gatgtctcgc
       61 ttcacgagat gccggtgttg acgacggcac ccgtgcgcgc cgcttcttgc accgcgtcgc
      121 acacctgaat cgtcttgagc ccagcgcgcc cgtcgcatat cggctcggct tcgccgcgga
      181 tcactgcggc gaagtgcgcg atctggtttg ccagcggatc ttcgacggca acgtcgatgc
      241 ggctgtcgtc agtggcagac caccacgact gcactccggg atacgtcttg agtcgcatgg
      301 tcgggatcga gagcgaccca accgtgccca tgacgtgata gctgtcctcg tccggatagg
      361 tggcgtagga ggtgttctcc ccgctggttt gctcccatga tcgaggagag gctgcggtat
      421 cggagagcat gaaggtgccg agcacgccgc cagcgaagtg aaagatcatg gcggcggtgt
      481 cttcgacctc gaagccccgg gtcgcagagc ttgtggtggc ctgcacagcg gtgatgtcac
      541 cggccagcgc catcaggttg ttcacctcgt gcgtgaggtt gagcaggatc gggccacccc
      601 cggccttgcg ccgccacccg tcgccgacat cgaagtagtc gtccggcttg tagaacgtgg
      661 cctgaccgac gatagccacc aagcgtccga gtacgcctga actgatcacc tcacgggcgg
      721 cggccatgtt ggcggagtag ttgcggtgat ggccggtgag cagcggaacg tgagcagcct
      781 ctgcggcctc gacgagctgc ttggccccgg cggcagtctc ggccaaaggc ttctcgacga
      841 tgaccggcac ccccgccgcg atgcaggtca agccgccttc gacatggagg cgattggggg
      901 tggcgaggat cacgccgtcg ggccgatcgt cggccagcgc tcgctcgaga gtgacgtaat
      961 gaggcacccc gagctcggcg gcgagggcac cggcttccgg cgacggatcg atgatcgagg
     1021 ccaagctcgc gtgcggcgac ttgacgagct cttcgatgtg acggcgcccg atcagacctg
     1081 ctcctgcaac ggctatgcgc atgtcctgga gactaccgaa ttgctggcgc ggaatcggct
     1141 cagatgtcgg agtcggggct gaatgtcatg ctggtcagaa atggttcgca gccgggcttg
     1201 cccatccgcc agcgggccag gccgggccgg tcacgggacg accgctgccg gcactcccgt
     1261 caacaggctc gcctcgaaga tcggtgacgg ggtccgtgtg cgacatgctt cgccgtaagc
     1321 ctgcagcagc cgctcggtcg tggctcccca cccgaaactg cgcgcgtggc gcacggcgcg
     1381 gcggcccaac tcggcccgca ggccggggtc ggcgagcatc cggtccaggg ttgccgccca
     1441 gtcgtcgaca tcgtgcccgt cgacgagcac tcccccagga ccgaccgccg tcggcagccc
     1501 gccgacccgc gcggcaacca ccggggtccc gcacgcgagc gactcgatcg cgaccaggcc
     1561 gaacgactcg ttgtaggagg ggacggtcac caggtcggac gcgcggtacc actgcgcgag
     1621 agtcgcccgg tcggccggtg gcacgaagcg cacgtatgcc gtcatcccca gctccgcggc
     1681 cagcgtggcc aggctctcgg ggtgttccaa accggtgccg ctcgggccac cgaggatgcc
     1741 gacgaccagg tcgtcgggcg tccagtcggg gtgcgcgtcg agcagccgcc gggccgcctt
     1801 gacgagcagg tcgggagcct tgagcggctg gatgcgaccc acgaagagga ccacgtgcgc
     1861 accctgcggg agcccgaggc gggcgcgcgc ggcataccga tcgccggggg tgaagacctt
     1921 gaggtcaacg cccgggggta cgacgaccac gcgggccggg tcggcgtcgt agagcgacac
     1981 caactggcag gcttcgtcgg cggtgttggc ggtgagcagg tcggcggctt cgacgacctg
     2041 ggcctcgccg atctcgcggc cggccggctc gggacgctcg ccgtcggcga gcgcggcgtt
     2101 cttgacccgg gccatcgtgt gcatcgtgtg gacgagcggg acgcgccacc ggtcggccgc
     2161 cagccagccg acctggccgg agagccaata gtgcgagtgg acgaggtcgt accagccttc
     2221 agggcgggcc gcgccggcgc gcatgacgcc tgcggcgaac gcgcacagct gaccgggaag
     2281 gtcgttcttg gccaacccct catacggccc cgcggtgatg tgccggacgg taactccggg
     2341 gacgagctcg accgtgggcg ggagggcgcc ggtcgtggcc cgcgtgaaga tctcgacctc
     2401 gcggttctgg cgggccaact ccttggcgac ctcggcgatg tagacgttca tcccgccggc
     2461 gtcaccggtg cccggctgct ccagcgggct ggtgtggacg ctgaacatcg cgacccgacg
     2521 gatctcctcc ggcgtcacag ctccccgatc ctttccacag atgcacagaa gatgcacaca
     2581 tgtcagcggc acacgtttct cgacaacacc gacgccgctg tcgagattcc cgggcggcat
     2641 acgctgcggt gtgtgaaccg caccagagcc gagggtggac cccctttggg caggatcacc
     2701 cgagggacga cgaaccccaa tcggctgcgc cgctgcgacc gttggctggc cggaacgcag
     2761 gcctggcggc tgcgcgcgcc gatggacccg ccggtggtcg tggacctcgg ctacggtgcc
     2821 tccccggtga cggccgtcga gttgttcgag cggcttcgcc gggtgcgcgg cgacgtcgag
     2881 gtgctcggcc tggagatcga cccggagcgc gtggccgccg ccaccgggtt ggagcggccg
     2941 gggctgagtt tccgacgcgg cggcttcgag gtgccgaccg ccgacgggcg agcagtgacc
     3001 gtcgtgcgag ccttcaacgt cctgcgtcaa tacgaggagg acgaggtcga ggcgatctgg
     3061 gccaaggtgc gttcacggct ggctccggac ggggtcctca tcgacgggac ctgcgacgaa
     3121 ctcggtcggc tcgcgtcgtg gatcgccgtc gagcccgcgg ccggcccagt cagcctcacg
     3181 ctgtcgatgc gactgcgcga gctgagcact ccggccgacg tggccgagcg gctgcccaag
     3241 gccctcatcc accgcaacgt ccccggcgag cgggtccacg gcttcctcgt cgcacttgac
     3301 caggcctggc ggtatgccgc gccgcaggcg gcatacgggg tgcgcgcgag gttcctggag
     3361 acggcggcgc ggatgcgggc caacgggtgg cccgtgctcg acgggcccgc gcgatggcgc
     3421 ctcggggagc tgagcatccg ctgggatgcc gtccggcccg ggtgaccttc tgcgcagcgt
     3481 gacctacgcc tcggcgtggt cggatccttc ggcgtcgtcg tggcgggcgt aatcgtcctc
     3541 gagtcgctcg atgtcgctct cgtcgaagaa gccgaacccg acctcaagga tgcgcccggc
     3601 cttctcgccc gggttggcga tccggtgggt cgcaccgcgc ggcacccaga cggtctcgcc
     3661 gacgcccgcc gtccacgtgc ggtcatcgac ggtgacatcg atcggcacgt cgagcatcgt
     3721 ccacatctcg ccgcggtgct cgtgccgctg caggctcagc cggtggccgg cctcgacaag
     3781 gatcgtcttg accgtcacgg tctcgttgga cacgaattgg gtgaacgacc cccacggccg
     3841 gttggccacg aaaatgtcca cccgtgggtc gcgaaggcct tcgacgtatt cggtgcggtg
     3901 gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgccca gggcggccag
     4021 acccaggacc aggacgacca cgccctgaac cgaagagatc gaggaaaaca tgggtggcct
     4081 tccgacggcg tgagtgaacc tcttagacgt ccagcaccag ggtaaccggg ccgtcgttca
     4141 ccagatgaac cctcatatgg gcaccgaagg ttcccgtggc gacccgcagg ccctctgcgc
     4201 gcagggcctc ggcgacggcc tcgacaagcg gctcggcgac cggtccgggc gccgcggccg
     4261 accacgacgg ccgccgaccc ttgcgcacat cgccatagag ggtgaactgg ctgacgacca
     4321 ggaccggcag ccccgcctcg accgcggacc gctccgcccc cgggctctca tcgccgtccg
     4381 gcccgtcgaa gacccgcaga tgggcgatct tgcgggccat ccactcgacc tgggtcgggc
     4441 cgtcgccatg ggtgacgccg acgagggcga ccaggcccgg gccgtcgagg tcgccgacga
     4501 cgacgccgtc aacctcgacc cgcgcggcgg agacccgttg caggacgacc cgcacggcgg
     4561 gctcagatcc cgacggcgac gatcgagccg accggctgac cgtggccgag gacgggagcg
     4621 tcctcgaggg cggcataccc cgcctcggac ccgaccccga tgcggcgcag gatcgccgcg
     4681 aggaccatcg gcttggcgcg catgtgaccg tcggcgatct tgacggcgac gccgcgaccg
     4741 tcacccagcc ccacggcata caccgactcg gcgccgtcct tggcgatgag gcgctggtcg
     4801 gcgggagtct cccggatgag cgtggtgacg ccgcggcggg tgccgccgag gtattcgggg
     4861 tgggcgcgga tcgcgtcggc gacccgcgcc tcggggctgc ccgcgggggc cgcggcgatc
     4921 cggccgaacg cgtgcgcaag ccccgtcagg ggtatgccgt gcagcggcgc cccgcagccg
     4981 tccaccgcga tcgcgccgac cgcggtgccg gagaggcggg caaccgccgc ggtgatggcc
     5041 tgctggagcg ggtgctcggg gtcgaggtag gtcgcgaggt cccacccgtt ggcggcagtc
     5101 gtggcgagca tgccggagtg cttgcccgaa cagttctgtg cgagtgaggt tttggggtgc
     5161 ccggcgcgga tccactcgtc gcgcgcctgc tcgtcgaccg ggtagtcggg ggtgttgagc
     5221 aggtgggtct cgtcgagccc ggcaccgtgg aggatctcga gggccgcggc gaggtggaag
     5281 gcctcgccgg agtggctggc ggcgacgagc gcgagctgat ggggcgcgat gtcgaggccg
     5341 gcggcgacca tggctgcggc ctggatcggc ttgctcgccg accgggggaa gatcaggccg
     5401 gtcgggtcgc cccacgcctg ctcgatcgag ccgtcggcgc tggtcacgac ggcggagcct
     5461 cggtggacgc tctcgacgac gccgcaccgc acgacgtggg cgagcacggg gccctgcgtg
     5521 agcgcgggcg aaacggggtg tgcggctgcg tggctggacg ggttcgacac gcgcgccagt
     5581 gtgccagagg caggtgcccc gcggacccga aggcggcggg acggcatacc cccaagaccc
     5641 cgggtatgcc ggtgggccgg cttccctggg aaaccggccc acctctgtgc acccccccta
     5701 cggggagttc cgcctgtggt cgaggcggat gccgcccccg acgacagggt tgatcagtcg
     5761 ccgaccttct tggcagccgt cttggcggcg gtcgtggtct tggcggcggt cttgcggaca
     5821 ccggtgccgg tggcgcgggt gcgagcggtg gtgcgcttga ccccggcctg agccgtcgtg
     5881 gtggtggtgc gggccgcggc gcgggtccgg gcggcggagg ccttgacggt cgacgcgacg
     5941 gtctcggcgt ccttctcgac agtctcggcg accttggcgg cgacgacctt ggcctcttcg
     6001 gcggcggact tgatggtggc cttggcggcg cgctcggtgg acacggcggc cttgcgggcg
     6061 gtggtgacgg cacccttgcc agcggcaacg gtggtctcga cctgcgactt gaggtcctgg
     6121 gtcgagcgcg tggtgcggat gcgctgcacg agggcctcgc cgcgctcggc gagctcgtcg
     6181 accgtggcct gggccttggt ggccaactcg atgctgtgac cgacggcgtt ggccgggacg
     6241 gtctggacgt ccttggccgt cttggagaga cgcttggtca ggtcggcctg gatcttggcg
     6301 aggttcttct ggaccttctt gacgtccttc tggaagcgct cggcgtcctt ctgcaggctc
     6361 ttggcgtcca tctcgcgggc gacctggacg aagtgctcct gggccacgcg gatcttctcg
     6421 acggccaggt cggtcgcgcc gacgacggcc atgagttggg tgcgggcctc agtggcggtc
     6481 ttctgcaggt ccttcttcat ttcgtccatg cgggtcttgc gggccatggt gggtaatcct
     6541 cctcaggatt ctggtgtctc ggtggactgt tctgcgggtg cgggtgccac gtctccgtct
     6601 gatgcggacc ccgtggccgg ggcagttctg ggttcgtcgg cgatgtccac gaacgagcgg
     6661 tagacatcga cgaggacctg gcgttggcga tcggtcagcc gaccgtcggc ggcgatcgcg
     6721 gccaggacac tgggtgcgct cgtggggctg gactcgtcga ggatgccggc gcggacatag
     6781 agcgactcgg ccgacacctc caagccgcga gcgatctgtt gcaggatctc cgcgctgggc
     6841 ttcttgagcc cgcgttcgat ctgggagagg taggggttgg agatcccggc caactcggac
     6901 agctgccgca acgagagctt ggctgcccgg cgctgttcgc gcaggtagct gcccaagtcg
     6961 agcttgaggg tcggggtcat gacaccatta tgcttgctct agttagcaaa acgcaaactg
     7021 gaggcagcgg gccgcgcgga tgcgaggatg acgcgcatgg cgggactcct ggtggcaggc
     7081 acgagctccg acgccgggaa gtcgctcatc gtcgccggac tgtgccgggc gtgggcgcgc
     7141 caaggcgtgc gggtggcgcc gttcaaggcg cagaacatga gcaacaactc gatggtctgt
     7201 gccgacggct cggagatcgg gcgcgcgcaa cacctgcagg cgatcgccgc cggagtcgag
     7261 ccctccgcgc tgcacaaccc cgtgctgctc aagccgtcga ccgaccgccg cgcgttcatc
     7321 gtcctgcgcg gtcagccggg cggcacattg gaggcgggcg agtacgtcgg cgggcggacg
     7381 cggttggctg acgcggcata cgaggcgtat gccgtgctgg cgggaactca cgagttggtc
     7441 gtctgcgagg gggccgggtc gccggcggag atcaacctgc gggcgggtga ctacgtcaac
     7501 ttcgggctgg cgcggcggtt cggactgccc acgctgctcg tcggcgacat cgatcgcggc
     7561 ggggtgttgg cgtcgatctt cggccactgg gggatcgtcg acgactctga tcgggcgctg
     7621 ctggcggggt atctcatcaa caagttccgc ggcgaccagt cggtgctcga ccccgggctg
     7681 gacgagctga cccggcgaac cgggttgcgg tgcttcggag ttgtcccgtg gctggaccag
     7741 gtgtggctcg actcagagga tgcgctcgct ttcgcgaagt ggccgcggtt gccggggcgg
     7801 gccgggacgc tgcgggtcgc ggcgatccgg ttgccgcgga tctccaacgc caccgatttg
     7861 gacgccatcg cgtcggagcc gggggtcgat gtgttcgcca ccgccgagcc cgccgacatc
     7921 gagcatgcgg acctcgtcgt gttgccaggg agccgggcca cggtggccga cctggagtgg
     7981 ctgcgtcggc tcgggctggc cgatgccctg gtgcggcggg cggcggaggg gcggccgatc
     8041 ctggggatct gtgggggtta tcagatgctc acggccgaga tcgacgaccc ggtcgagtcg
     8101 ccgagcgggg gtgctgccgg gcttgggctg ctgccggcgc gggtgcggtt ccagccggag
     8161 aaagtgttgg ggcggccgcg gggtaggtgg cgggggcatg acgtggtcgc ctacgagatc
     8221 catcacgggg tggttgagcc cttggcgggt tctggcggcg agggcggttt gggcggcgaa
     8281 cgcagcgaga ggggcgcggg gagcgagccg ttcctggacg gcatacgcga tggcgctgtg
     8341 tggggcacga tctggcacgg ggcgtttgag aacgacggct tccggcgcgc atggctcgcc
     8401 gagatcgccg ccgccgtggg gagcacgtgg gtgccgcgcg ccgacgcgcc ggggttcagc
     8461 gagcggcgcg agcggatgct cgatgacctg gccgacgcgt tggaggcgtg cgtcgatctc
     8521 ccggccctgt tggccgcggc caggggcg
//
LOCUS       DAORPW010000096         8414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2053_length_8414_cov_559.857040, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000096 DAORPW010000000
VERSION     DAORPW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2053_length_8414_cov_559.857040"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..990)
                     /locus_tag="PLB86_08600"
     CDS             complement(4..990)
                     /locus_tag="PLB86_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HRC65234.1"
                     /translation="MSPVDIEEAVRRYAASWPSLVPVADAFVTTVTGILDDAGINYLT
                     VTGRAKAVASFAAKAARERDGARAHPDPMRDITDQVGVRVITYVRSDVDAVAELLADQ
                     LTVLDDRDMGRETARAGRFGYASRHLLVSLDSGEHTAYEPTRCASIQLRTVLQHAWAE
                     FEHEIRYKGTVPTEFASDLDRRFTLAAGLLELADREFSTIRDTIAAELGGGETPEADD
                     RDPRISAQELTTFLAGRYASAGWSRTEHYEWLSGLLLELGITSLDELSATLRDIDTVG
                     VTERMDYRYPAGAVRRLDDDLLAKVGEPYLQLHGNAHRVEGLRARLAKLRGE"
     assembly_gap    1165..1264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1282..2256
                     /locus_tag="PLB86_08605"
     CDS             1282..2256
                     /locus_tag="PLB86_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65235.1"
                     /translation="MTLRGLTKSYGTTEVLVDVSATLEPGRIYGLLGANGTGKTTLLA
                     CIAGHTFRDGGEIRIDGHDPLEHAPTLARLCFVHEDQAYNDAFSVADILAVMPTFYPE
                     WDDEIATRLMRRFRIPRRTRSRKLSRGQRSALAIVLALASGAAYTFLDEPYLGLDATA
                     RGIFYDELLAVYAASHERAAAGTTAPRTFIMSTHLIDEAAELIEEVLVLDRGRIVLQA
                     DVEDARRTAFLARGFEADVLALAAGRELLSTRRLGGIVTATIRGELSSDERQRAEAAR
                     VSIEPTTLQEFVAAIGQHGESDQPAPHLAPHPAPLPASQSASESRSLQ"
     gene            2253..2972
                     /locus_tag="PLB86_08610"
     CDS             2253..2972
                     /locus_tag="PLB86_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65236.1"
                     /translation="MITYSRRSFRLYLANRQYVLAVPIYTLGLMLLLSILIVIVVGIA
                     TGFPVSKDFEEGFRANSGAILSLPGFLVSVGVLAATRNFAMALAFGSTRRHFWLGTTA
                     GFVVTAAVTAAAAVLMLGLERLTGHWFVGAYAFDVAVLGNGNIAQTFSVTFVLALLSL
                     TVGALFGTVLRAFGTKATAVLSIGVLLILFAAVALVVWKWSVVAPLLASWGAWATVII
                     GAAFLVLASIGSYAACRRATV"
     gene            complement(3102..6068)
                     /locus_tag="PLB86_08615"
     CDS             complement(3102..6068)
                     /locus_tag="PLB86_08615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRC65237.1"
                     /translation="MRRRIAGLAALSLAVAPLVTTLGALSAAAAPQKKSYIVVMKADP
                     LVRSMSAKSLGTPTAKSRKGDLRRVQDKVLAAAGLPASAKSTSYTNALNGFAVRATDA
                     QVDSLSKRPEVAAVLVDELRQPTAHDTSRSIAVDRGGPGNRLYDFLGLDGRSESWRSG
                     LTGAGVTVGVIDTGIWPEHPSFADDGSYPVHAPLDTSVGSACAFGNTAHNPGDAAFTC
                     NNKLVGARVFLDTYRANSGLTAEEFDSARDNEGHGTHTASTAAGNANVQAQILGVDKG
                     MTSGIAPRAQVIAYKALGEGGGYTSDLVAAIDQAVADGVDVINYSIGGGAATVAADTL
                     AFLFAADAGVYSAVSAGNSGPGAKTIGGPADVPWVTSVGANTQPTFYQGTIEAGGKRY
                     LGASVTKGVGPAKFVDAAAAGSELCLEGQLNPAKVAGAIVLCKRGGNGRVAKSAEVLR
                     AGGVGMVLFNAVDVDTLFSDTFFVPTVMIDFTPGTQLKAWIAGTPNPVGRLVSGGIGT
                     SPFYSPTMAYFSSRGENPTAGDIIKPDITAPGVQIMAGNTPKPSGNSQPSGELFQAIA
                     GTSMSSPVVAGSYALLKQAHPTWSPAAAKSALMTTANTAVRDNDRVSAAGPFAMGSGM
                     VDLGEPRSRGSAYQPGLVYDAGFTDYLGFLCAEGPSVFGNPAATCASLAASGIPTRAA
                     DLNLSSIGVEAVAGSTTVTRTVTSVADRRTSFEAKVQAPAGYRVTVTPSRITLAPGAS
                     ATFTVTITNTGSAPAGQWRTGALTWRGDGYEVRSPIAVKGTTLSAPTSVSGTGTSGSA
                     SLALQFGFAGPYTATAEGLLAATHTPGTIAQDPDTTYPSPDDGAGVVSVPFTLSGVGV
                     AKWALNMPDPTDLDIYLFGPGGNLVGASTNGGTKESILLNSPADGTYTMVIHGWAVGA
                     TPLQFDLRSWLVPAEDGAGSLSISSGASTTATIGGSHTVGIAWTGLTAGEEYFGSVTH
                     RNGTAVVGRTVVSVTG"
     gene            complement(6193..6657)
                     /locus_tag="PLB86_08620"
     CDS             complement(6193..6657)
                     /locus_tag="PLB86_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65238.1"
                     /translation="MRRTIWLVGVMGIWVLALGACATSVSGPASAPSSGSLDPATLRD
                     QLRSAAASLKPTAGYVPTPAASGLDPALGAYVAIARSDLAARLSIAPDRIGVATARVV
                     TWPNAALGCPKPGMAYADQVIDGALIILRVDERSYEYHSGGDTAPFLCETVG"
     gene            6719..7714
                     /locus_tag="PLB86_08625"
     CDS             6719..7714
                     /locus_tag="PLB86_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC65239.1"
                     /translation="MGIDKTDDSAMAQQYAVVAASQRLGRPFYTPQPQASSIADLRHD
                     DPGERCDLWGLYVGPDMVGTVTLWLPLHDNTDTVWLQLDVHPDHRGRGYGGLAVAAVV
                     DFARDLGRTRVVTTARYPADRPVDHPYRRFAEQHAFRLGSLDVNRRLPLPVPAPVLSG
                     LAERARAAYLPAYRIETFTRVPDALLAPLCACMSRVESDAPSGELEWEEEALTPERLR
                     GFAELDEHVGRLRITSVAREAESGEVVGYTELFIIGGTTKALQSGTFVVSEHRGHRLG
                     LGLKVANLVALQAHRPDAVEIFSINNAENTWMVAVNEELGFEPVELVGQFYRQLP"
     gene            complement(7731..8381)
                     /gene="idi"
                     /locus_tag="PLB86_08630"
     CDS             complement(7731..8381)
                     /gene="idi"
                     /locus_tag="PLB86_08630"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRC65240.1"
                     /translation="MTDVSAVVDVPPESADLSGELVVLVDDGGAAIGVAPKASVHGAD
                     TPRHLAFSCYVIDASGRLLVSRRATTKPTFPGVLTNSLCGHPGPGESLPDAVCRRAGS
                     ELGLDLAALPIRLVLPQFSYRAEMNGVVENEACPVFVVTVPDGIPLAPDADEVASVEW
                     MPWAAYRDRVLDGSLQVSSWSTDQVRQLVALGADPATWPEAPASDLPPAAQPHPAG"
BASE COUNT         1297 a         2806 c         2873 g         1338 t
ORIGIN      
        1 ggctcactcg ccgcggagct tggccaatcg ggcgcgcagc ccctcgacgc ggtgggcatt
       61 gccgtgcaac tgcagatagg gctcgccgac cttggccaac aggtcgtcat ccagacgccg
      121 cacggccccg gcggggtagc ggtagtccat ccgctcggtc accccaacgg tgtcgatgtc
      181 acgcaacgtc gcgctcaact catcgagcga ggtgatcccc aactccagca gcagcccgct
      241 cagccattcg tagtgctcgg tccgcgacca gcccgcgctc gcatagcgcc ccgcgaggaa
      301 ggtcgtcagc tcctgcgcgc tgatccgggg atcgcgatcg tccgcctcgg gggtctcgcc
      361 gccgccgagc tcggccgcga tcgtgtcgcg gatcgtcgag aactcgcggt cggccagctc
      421 cagcagccca gctgccagcg tgaaccgtcg gtccaggtcg gaggcaaact ccgtgggcac
      481 cgttcccttg taacggatct cgtgctcgaa ctcggcccag gcatgttgca ggacggtccg
      541 caactggatc gacgcgcacc tcgtcggctc gtatgccgtg tgctcacctg agtcgagcga
      601 caccagcagg tgcctgctcg cgtaaccgaa ccggccggcg cgggcggtct cgcggcccat
      661 gtctcgatcg tcgagcaccg tgagttggtc ggccagcaac tcggcgacag cgtcgacgtc
      721 actgcggacg taggtgatga cccgaacccc gacctggtcg gtgatgtcac gcatcgggtc
      781 gggatgcgcc cgggcgccgt cgcgctcgcg ggcggccttg gcggcgaacg acgcgacggc
      841 cttggcccgg ccggtgacgg tcagatagtt gatgcccgcg tcgtcgagga tgcccgtcac
      901 cgtggtgacg aaggcgtcgg cgacagggac cagcgagggc cagctcgcgg catacctgcg
      961 cacggcctcc tcgatgtcga ccggactcat gaacgaactc tcccacgccg cgcgagcgac
     1021 ccgcttgaca ccccgccgcc accatggtta gttagtggag taacgaacca ctgaaggaac
     1081 cactgaagga ggcgggatga cggactggac cgacgggcgg ccgatcttca tggacctcgc
     1141 cgaccggatc gccgccgaca tcctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnccacgg caagcgccgg gatcaccctg cgcggtctga ccaagagcta cggcacgacg
     1321 gaggtgctcg tcgacgtctc ggccaccctg gagcccgggc ggatctacgg gctgctcggc
     1381 gccaacggca cgggcaagac cacgctgctc gcgtgcatcg ctgggcacac cttccgcgac
     1441 ggcggtgaaa tccgcatcga cggccacgac ccgctggagc acgctccgac cctcgcccgg
     1501 ctgtgcttcg tgcacgaaga ccaggcctac aacgacgcct tctcggtcgc cgacatcctc
     1561 gcggtcatgc ccaccttcta ccccgagtgg gacgacgaga tcgcgacccg cctgatgcga
     1621 cgcttccgga tcccgcgccg cacccgatcg cgcaagctct cccgcggcca gcgctccgcc
     1681 ttggcgatcg tcctcgcgct cgccagcgga gcggcataca ccttcctgga cgagccctac
     1741 ctcggactgg acgcgaccgc gcgcggcatc ttctacgacg aactgctggc cgtgtatgcc
     1801 gcgagccacg agcgggcggc ggccggcacc actgcgccgc gcacgttcat catgtccacc
     1861 catctcatcg acgaggcggc cgagctgatc gaggaggtgc tcgtcctcga ccgcgggcgg
     1921 atcgtcctgc aggccgatgt cgaggacgcc cggcgcaccg ccttcctggc gcgcgggttc
     1981 gaggccgatg tcctcgcgct cgcggctggt cgtgagctgc tctcgacgag gcggctcggc
     2041 ggcatcgtca cggcgaccat ccgcggcgag ctgagctccg acgaacggca gcgcgccgag
     2101 gcggcgcggg tgtcgatcga gcccaccacc ctgcaggagt tcgtggcggc gatcggtcag
     2161 cacggcgagt ccgaccagcc cgctcctcac ctcgcgcccc acccggctcc cctccccgcc
     2221 agtcagtccg cgtccgaatc gaggtcactc cagtgatcac ctactcccgc cgctccttcc
     2281 ggctctatct cgccaaccgc caatacgtgc tggctgtccc gatctacacg ttgggcctca
     2341 tgctgctgct cagcatcctc atcgtcatcg tggtgggcat cgcgacgggc ttccccgtgt
     2401 cgaaggattt cgaggagggg ttccgggcca actcgggcgc gatcctgtcc ttgccgggct
     2461 tccttgtctc cgtgggagtg ctggcggcca cccgcaactt cgcgatggcg ctggccttcg
     2521 gcagcacccg tcggcacttc tggctgggca cgacggccgg gttcgtcgtc actgccgccg
     2581 tcaccgcggc cgcggcggtg ctgatgctcg gtctggagcg gctcactggc cactggttcg
     2641 tcggcgccta tgccttcgat gtggcggtgc tcggcaacgg caacatcgcg cagacgttct
     2701 cggtcacctt cgtcctcgcg ctgctgtcgc tgaccgtcgg cgccctgttc ggcaccgtgc
     2761 tgcgggcgtt cggcacgaag gcgacggcgg tgctcagcat cggtgttctg ctcatcctgt
     2821 ttgcggccgt cgcgctcgtg gtgtggaagt ggagcgttgt cgcgccgctg ttggccagtt
     2881 ggggagcatg ggccacggtc atcatcggtg cggcattcct cgtgctcgcg agcatcggct
     2941 cgtatgccgc gtgccgccgc gcgaccgtgt gaccgagtga ccgagtgacc gagtgaccga
     3001 gtgaccgtcg cgctcgggcc gagcgcgacg ggctcaggcc caacgccgag gacgaggagt
     3061 catcgacgcc ggacccgagc ccgatcgctg tcggaggttg gctaaccggt tacggacacc
     3121 acggtccggc cgacgaccgc cgtgccgttg cggtgcgtca cgctgccgaa gtattcctcg
     3181 ccagcagtga ggccggtcca ggcgatcccc acggtgtggc ttccgccgat ggtcgcggtt
     3241 gtgctggctc cgctgctgat cgacagactt ccagcgccgt cctctgccgg aacgagccaa
     3301 ctacgcaggt cgaactgcag cggggttgca cccacggccc aaccgtggat gaccatcgtg
     3361 taggtgccat ctgcggggct gttgagcaag atcgactcct tcgtcccacc gttcgtgctc
     3421 gcaccgacga ggttgccgcc tggaccgaac agatagatgt ccaggtcggt cggatcgggc
     3481 atgttcagtg cccatttggc cactccgacc ccgctcaggg tgaacgggac agacaccacg
     3541 ccagcgccgt cgtcagggct ggggtaggtc gtgtccgggt cctgagcgat ggttccggga
     3601 gtgtgcgttg ccgccagcag cccttcggcg gttgcggtgt aagggcctgc gaagccgaat
     3661 tgcagagcca gcgacgccga tccggaggtg ccagttccgg acacgctcgt cggagccgac
     3721 aaggtcgtcc ccttgacggc gatggggctg cgaacctcgt atccatcgcc cctccacgtg
     3781 agtgcaccgg tgcgccattg gcccgccggc gctgatcccg tgttggtgat ggtcaccgtg
     3841 aaggtcgcgc tggccccggg cgcaagggtg atccgcgacg gagtcaccgt cacgcggtaa
     3901 cccgccggtg cctggacctt ggcctcgaac gacgtccggc gatcggcgac gctcgtgacc
     3961 gtgcgcgtca cggtcgtcga cccggccact gcttccacgc cgatggacga gaggttcagg
     4021 tcggccgccc gggtggggat accgctcgcg gccaacgacg cacacgtcgc cgccgggttc
     4081 ccgaacactg acggaccctc ggcgcagagg aagcccaagt agtccgtgaa ccccgcgtcg
     4141 tagacgaggc cgggctggta ggccgagcct cgggaccggg gctcacccag atccaccatt
     4201 ccggaaccca tggcgaacgg cccggccgcg ctgacccggt cgttgtcgcg gaccgctgtg
     4261 ttggctgtgg tcatcagtgc ggacttggcc gcggcgggtg accaggtggg gtgtgcctgt
     4321 ttgagcagcg cgtaactccc ggcgacgacc gggcttgaca tcgaggtccc ggcaatcgcc
     4381 tggaagagct cacccgaggg ctggctgttg cccgatggct tgggcgtgtt gcctgccatg
     4441 atctgcactc cgggtgcggt gatgtcgggc ttgatgatgt ccccggccgt cggattctcg
     4501 ccgcgcgagg agaaatacgc catcgtcggg gagtagaagg gggacgtccc gatgcctccc
     4561 gagacgagtc ggccgaccgg gttgggcgtc ccggcgatcc aggccttgag ctgggtgccg
     4621 ggggtgaagt cgatcatcac cgtcggcacg aagaacgtgt cgctgaagag ggtgtcgaca
     4681 tcgaccgcat tgaacaggac catgcccacc ccaccggccc gcagcacctc ggcgctcttg
     4741 gccacgcgcc cgttgccgcc gcgcttgcac aggacgatgg cgccggcgac cttggccggg
     4801 ttgagttgac cctcgaggca caactcactt ccggcggcgg cggcgtcaac gaatttcgcc
     4861 ggcccgacgc ccttggtgac ggaggcgcca aggtagcgct tgccgccggc ttcgatggtg
     4921 ccctggtaga aagtcggctg cgtgttggca cccactgagg tgacccacgg aacgtccgcc
     4981 ggtccaccga tcgtcttggc tccggggccg ctgttgccgg ccgagacggc cgagtacacg
     5041 ccagcgtcgg ccgcgaaaag gaaggccaac gtgtcggcag ccacggtcgc agcgccgccc
     5101 ccgatcgagt agttgatgac gtcgaccccg tcagcgacgg cctggtcgat cgcagcgacg
     5161 aggtcggagg tgtagccacc gccctcaccc agggccttgt aggcaatcac ttgggcccgc
     5221 ggagcgatgc cggaggtcat tcctttgtcg actcccagaa tctgggcctg gacgttggcg
     5281 ttgcccgcag cggtcgaggc cgtgtgagtt ccgtgtccct cgttgtcgcg agcggagtcg
     5341 aactcctcag cagtcagccc gctgttggca cgataggtgt caaggaagac gcgggcgccc
     5401 acgagcttgt tgttgcaggt gaaggcggca tcgcccgggt tgtgggccgt attgccgaag
     5461 gcgcacgccg agcccacgct cgtgtcgagc ggcgcatgca cggggtagct gccgtcatcg
     5521 gcgaaactcg ggtgttcggg ccagatcccg gtgtcgatga ctccgacggt gacgccagca
     5581 ccagtgaggc cggagcgcca cgactcggag cgtccgtcga ggcccaagaa atcgtacagc
     5641 cgatttccgg ggccgccccg gtcgaccgca atcgagcgag aggtgtcatg cgcggtgggc
     5701 tgacgcaatt cgtcgaccag caccgccgca acctcagggc gtttggacag tgaatcgacc
     5761 tgggcgtcgg tggcccggac cgcgaagccg ttgagggcat tggtgtagct ggtggacttg
     5821 gccgaggcgg gcagtcccgc cgcggcgagc accttgtcct gaactcggcg aaggtcgccc
     5881 ttgcgcgatt ttgccgttgg ggtgccgagc gacttggcgg acatcgaccg gaccagaggg
     5941 tcggccttca tgaccacgat gtaggacttc ttttgcggcg ctgcggccgc agacagggct
     6001 cccagggttg tgaccagggg tgcgactgcc agggagaggg cagccagacc ggcaatccgt
     6061 cgtctcattc catcctccgg gtgctggatg agcacggcaa cgaacacggg tcgatatatc
     6121 ccgagttccg tgcgggtggg gcgaccctat tcccgaggag ggccgggcgc caccgttttg
     6181 ggtcggagaa ggtcagccga ctgtctcgca caggaagggg gcggtgtccc cgcctgagtg
     6241 gtattcgtag ctccgctcat cgacgcgaag gatgatgagc gccccatcga tgacctgatc
     6301 ggcatacgcc attcctggtt tcgggcaccc caacgccgcg ttcggccagg tgaccacgcg
     6361 cgcggtagcg acgccgatgc ggtccggggc gatgctcaat cgggcagcga ggtcggagcg
     6421 ggcgatcgcg acgtacgcgc cgagtgccgg atcgagaccc gaggcagcag gcgtgggaac
     6481 gtaccccgcg gtgggcttga gggatgccgc ggcgctgcga agttgatcgc gcaatgtggc
     6541 cggatcgagg gagcctgagg aaggcgccga ggccgggccc gatacggatg tggcgcacgc
     6601 gccgagggcg agcacccaga ttcccatcac tccgaccagc caaatggtcc gccgcatacc
     6661 tcaggctaac tcccgcgaac ccgaacacct ccgccagtag cctgccgggg tggagatcat
     6721 gggcatcgac aagaccgacg acagcgcgat ggcgcagcag tatgccgtcg tcgccgcgag
     6781 ccagcgcctc ggacggccgt tctacacccc acagccacag gcgtcttcca tcgccgacct
     6841 gcgccacgac gacccggggg agcggtgcga cctctggggc ctatacgtcg gtccggacat
     6901 ggtggggacc gtcaccctct ggctgccgct tcacgacaac accgacaccg tgtggctgca
     6961 actcgacgtc cacccggacc accgagggcg tggctacggc ggtcttgcgg tggccgccgt
     7021 agtggatttt gcccgggacc tcggccggac ccgagtggtc accaccgcgc ggtatccggc
     7081 cgaccgcccc gtcgaccacc cctaccgccg gttcgccgag caacacgcct tccgtctggg
     7141 aagcctggac gtcaaccggc ggctgccgct gccggtgccc gcgccggtgc tgagcggcct
     7201 ggcggagcgg gcgcgggcgg catacctgcc cgcctatcgg atcgagacgt tcactcgtgt
     7261 ccccgacgcc ttgctggcgc ccttgtgtgc gtgcatgagc cgggtcgaat ccgacgctcc
     7321 cagtggggaa ctcgaatggg aggaggaggc gctgacgccc gagcggctgc gcgggttcgc
     7381 tgagttggac gaacacgttg ggcggctgcg gatcacctcg gtagcccgcg aggctgaaag
     7441 cggcgaggtg gttgggtata ccgagttgtt catcatcggg ggcacgacca aggcgcttca
     7501 gtcgggcacc ttcgtggtga gcgaacatcg cgggcatcgg cttgggttgg gcctcaaggt
     7561 ggcgaatctc gttgcgctgc aggcacatcg accggatgcg gtcgagatct tctcgattaa
     7621 caacgcggag aacacctgga tggtggcggt caacgaggag ctgggcttcg agcccgtgga
     7681 gctggtcggc cagttctatc gccaactgcc ctgagcgctc ccgggagggg tcagccagcc
     7741 gggtgcggct gagccgcggg cgggaggtcg gaggctggcg cctcgggcca ggtggcgggg
     7801 tcggcgccga gggccaccaa ctgtcgcacc tggtcggtcg accacgacga gacctgcagc
     7861 gagccgtcga gcacccggtc gcggtaggcc gcccacggca tccactcgac gctcgccacc
     7921 tcgtcggcat cgggagccag aggtatgccg tccggcaccg tcacgacgaa caccgggcag
     7981 gcctcgttct cgaccacgcc gttcatctcg gcgcgatagg agaactgggg cagtacgagc
     8041 cggatcggca acgccgcgag atcgaggccg agctcggagc cagcccgacg gcataccgcg
     8101 tcgggaagtg actcacccgg acccggatgg ccgcacagcg agttggtgag caccccgggg
     8161 aaggtgggtt tcgtggtggc gcggcgcgac acgagcagtc ggccgctcgc gtcgatgaca
     8221 tagcaggaga aggccaggtg gcgcggagtg tcagcgccgt ggacgctcgc cttgggagcg
     8281 accccgatcg cggcgccacc gtcgtcgacc agcacaacca gctccccgga gaggtcggca
     8341 gactctgggg gcacgtcgac cactgcggac acgtcggtca ttcaaacaga ggaacgtcga
     8401 ggtcgtaggc cagc
//
LOCUS       DAORPW010000097         8308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2094_length_8308_cov_605.091724, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000097 DAORPW010000000
VERSION     DAORPW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2094_length_8308_cov_605.091724"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1105
                     /gene="argC"
                     /locus_tag="PLB86_08635"
     CDS             59..1105
                     /gene="argC"
                     /locus_tag="PLB86_08635"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRC65241.1"
                     /translation="MMWTAAVAGASGYAGGEILRLLLGHPQIQIGAVTADSSAGKRLG
                     EVHPNLVPLGERVIEPTNPDTLAGHDLVFLALPHGASAALAAQLPDGVIVLDCGADFR
                     LVDEAAWTTFYDTPYAGQWPYGLPELPGVAGAKARTALTGATRIAVPGCYPTACSLAL
                     APGFTAGLLDPTDVVIVAASGTSGAGKSLKPHLLSAEVMGAMSPYGVGGVHRHTPEIE
                     QNLTAAAGSPVTVSFTPTLAPMSRGILATCTARLLSGADPAAVRAAWEAAYADEAFVH
                     LLAPGVWPRTADVLGSNSVHIQVTVDERVGRVIAVAAVDNLAKGTAGGAIQCANIALG
                     LPETTGLPVAGVAP"
     gene            1102..>1721
                     /locus_tag="PLB86_08640"
     CDS             1102..>1721
                     /locus_tag="PLB86_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HRC65242.1"
                     /translation="MTSTVNLPQGFRAAGVTAGLKASGRSDLALVVNDGPDHHAAAVF
                     TSNRVEAAPVTWSRQVVIDGRVDAVILNSGGANACTGSPGFLDTHRTAEKVGEVLGVS
                     AGDVVVCSTGLIGERLPMDLLLPGVDAVATALSVDGHDAAATAIMTTDTVSKQATFVG
                     DGWSVSGMAKGAGMLAPALATMLVVITTDAVTHPKEMDLVLRGATAV"
     assembly_gap    1722..1821
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1822..2130
                     /locus_tag="PLB86_08645"
     CDS             <1822..2130
                     /locus_tag="PLB86_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HRC65243.1"
                     /translation="ARAIARNNLFKCAVFGNDPNWGRVLAAVGTTRAAFDPARLAVTM
                     NGVQVCRDGGVGEDRSLVDLTPRHTSVIVDLHEGEAHATVWTNDLTHDYVHENSAYST
                     "
     gene            2151..3083
                     /gene="argB"
                     /locus_tag="PLB86_08650"
     CDS             2151..3083
                     /gene="argB"
                     /locus_tag="PLB86_08650"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRC65244.1"
                     /translation="MSSLRGVIDAAAVRVAASKAATLVEALPWLERFRGALVVVKYGG
                     NAMTDDTLKAAFAQDIAFLRYAGLHPVVVHGGGPQIGAMLKRMGLTSEFKGGLRVTTP
                     EVMEIVRMVLTGQVQRELVGLVNQHGALAVGLSGEDAHLFEARKRGTVVDGEEVDLGL
                     VGDVVGVNPAAVLDIIAAGRIPVVSTVAPDLDEPGQVLNVNADTAAAALAVALEARKL
                     VVLTDVEGIYADWPNRDSLLSQVTLSEARLLLERVDSGMVPKLEACIRAVEQGVPQAH
                     VIDGRAAHSVLLEVFTDEGIGTMVLPDPTGEVWA"
     gene            3080..>3396
                     /locus_tag="PLB86_08655"
     CDS             3080..>3396
                     /locus_tag="PLB86_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC65245.1"
                     /translation="MNDLAGGSARGDASGTSHTGEWLGRYAASLIPVFGVPQTVLSHG
                     QGCYVVDVDGKRYLDLLAGIAVNTLGHGHPALVDAVTKQASQLVHVSNFFTSQPQVEL
                     AEAL"
     assembly_gap    3397..3496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3518..3994
                     /gene="argR"
                     /locus_tag="PLB86_08660"
     CDS             3518..3994
                     /gene="argR"
                     /locus_tag="PLB86_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HRC65246.1"
                     /translation="MIPATPTARRAKIAGLLADRSVRSQGELLTLLADAGIEVTQATL
                     SRDLEALGAVKVRAGRGLVYAVPGASPSPVEQVPSRLRRLLEELLVTADASGNLVVLR
                     TPPGAANHLASTIDASHLTDVLGTIAGDDTILLVTSSAKGGRAVATRLLALAGASA"
     gene            4058..5515
                     /gene="argH"
                     /locus_tag="PLB86_08665"
     CDS             4058..5515
                     /gene="argH"
                     /locus_tag="PLB86_08665"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRC65247.1"
                     /translation="MNTERPGMPHEDSAGTSARVSLWGGRFAGGPADALAELSRSTHF
                     DWRLAAYDIAGSQAHARVLHRAGLLSDGDLATMLGALDELLVDVHTGAFVPSPEDEDV
                     HTALERGLIERAGADVGGRLRAGRSRNDQIATLLRMCLRDAARDLAAQVLDLVDALVG
                     QAQSHLGVAMPGRTHMQHAQPVLLSHHLLAHAWALLRDVDRLRDWDRRADSSPYGSGA
                     LAGSSLGLDPEAVARDLGFSRSVENSIDGTASRDVAAEFAFVMAMTAVDVSRIAEEIV
                     VWATKEFGYVTLDDAYSTGSSIMPQKKNPDIAELARGKAGRVVGDLTGLLTTLKGLPL
                     AYNRDLQEDKEPVFDAIDTLAVLLPAFTGMIQTLVFNRERLESLAPQGFALATDIAEW
                     LVRQGVPFRVAHEVAGACVRECEGRGIELWELTDAELAAVNPALTPAVREVLSVAGSL
                     ASRDARGGTAPVRVSEQLAEAQAAVAAARAFAAPA"
     gene            complement(5710..6666)
                     /locus_tag="PLB86_08670"
     CDS             complement(5710..6666)
                     /locus_tag="PLB86_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRC65248.1"
                     /translation="MPSTATTGQVTLRFLAAPTDAGQGGSVSGGRILEWIDKAGFACA
                     AGWSGRYCVTAYVGNIDFHRPVQVGDLVEVSAQLVLTGRSSMTIVVDVSSGNPRGGQV
                     EHAFECRMVFVAVDDEGRTVDVAQWDPPGAVLRAAQAEARRRIDLRERVKSLVAAQDW
                     EGATLAHGATLRFLAAPTDVNWGGKVHGGILMRWLDETGYVCASGWSQTHVVSRFSGG
                     VSFLRPIRIGDVVEIQSRLVHTGRTSMHVALRVLAGSPRTGELALATQCTSVYVALDA
                     DGRPCPVPTWTPTAAVDLAAQERALEFMALRADPAFHSSPRS"
     gene            6706..7323
                     /locus_tag="PLB86_08675"
     CDS             6706..7323
                     /locus_tag="PLB86_08675"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRC65249.1"
                     /translation="MSRLPSGFYDRPVLDVAVDLLGARISHGGVTIRLTEVEAYAGVG
                     DPGSHAFRGETPRTTVMFGAPGGLYVYFTYGMHWCANLVCGPDGQASAVLMRAGEVVD
                     GEEIARTRRGEACATRDLARGPARLASALGLTGEVNGTLTFEPDSPVRVQAAAEAPDP
                     ARIRTGPRVGVSGPGGDGTAYPWRFWIDGEPTVSVYRPGKPRRRP"
     gene            complement(7342..>8308)
                     /locus_tag="PLB86_08680"
     CDS             complement(7342..>8308)
                     /locus_tag="PLB86_08680"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRC65250.1"
                     /translation="GNALASGTTDWWDEPMEPLHDDEADTGSEVVLDLLRSQAPHLAA
                     LSLARISGTAGNTGSDNVLYRLGTELVVRLPRTPSAAASLRTETRLLPHLAGRLPVAL
                     PELVHLGSASARYPHPWAVLRWLPGRDAWADRATLDDPHGFRLAEDLAAVVDALGKVP
                     WAAYGDIVEVRPPGRRGGGLAPLTEDIDEWLSEAHGLVDERGVRASWQESLSAREWDG
                     DAVLCHGDLIPGNLLVQGNRLTAVIDWGGAGAGDPALDLVPAWAVLGPQGRERFRDGV
                     AADDDSWLRARGYALQQAVAGVIYYTPRRHPLADVMRRTLHAILR"
BASE COUNT         1174 a         2889 c         2815 g         1230 t
ORIGIN      
        1 gcgtccacgt ggcgggacgg gtcgacgcat accttctcgc cgctctgtat gatcatgcat
       61 catgtggacg gcagcagtgg cgggtgcgag cgggtatgcc ggaggcgaga tcctccggct
      121 gctccttggc cacccccaga tccagatcgg tgcagtcacg gcggattcct cagccggcaa
      181 acgactcggt gaggtgcacc ccaacctcgt cccgctgggg gagcgcgtca tcgagccgac
      241 gaaccccgac acgttggccg gccacgacct ggtcttcctc gccctgcccc acggcgcctc
      301 cgccgcgctc gcggcgcaac tgcccgacgg cgtcatcgtc ctggactgcg gggccgactt
      361 ccggctcgtc gacgaggccg cgtggacgac cttctacgac accccgtatg ccggccagtg
      421 gccttacggt ctccccgagc ttcccggggt ggccggggcc aaagcccgca ccgccctgac
      481 cggagccacc cggatcgccg tgccgggctg ctacccgacc gcctgctcgc tggctctcgc
      541 ccccggcttc accgccggac tcctggaccc gaccgacgtc gtcatcgttg ccgccagcgg
      601 cacctcaggc gctggcaagt ccctcaagcc gcacctgctc agcgccgagg tgatgggcgc
      661 gatgtcgccc tacggcgtgg ggggagtgca ccggcatacc ccggagattg agcagaacct
      721 caccgcagcc gctggcagcc cggtgaccgt gtcgttcacc cccaccctcg cgccgatgtc
      781 ccgcgggatc ctcgcgacgt gcactgcgcg actgctctcc ggcgcggacc cggcggccgt
      841 ccgcgcggcg tgggaggcgg catacgccga cgaggccttc gtccacctgc tcgccccggg
      901 cgtctggccc cgcaccgccg atgtcctcgg cagcaacagc gtccacatcc aggtgaccgt
      961 cgatgaacgc gtcggccggg tcatcgcggt cgccgcggtt gacaacctcg ccaagggcac
     1021 ggccggcggc gcgatccagt gcgccaacat cgccctcggc ctccccgaaa ccaccggact
     1081 tcccgttgca ggagtggcac cgtgacctcg accgtcaacc ttccccaagg cttccgggcc
     1141 gccggcgtga cggccgggct caaggcgagc ggccggtcag acctcgccct cgtcgttaac
     1201 gacggccccg accaccatgc cgccgccgtg ttcacgagca accgggtcga ggcggcgccg
     1261 gtgacctgga gccgccaggt cgtcatcgac ggtcgcgtcg acgcggtgat cctcaactca
     1321 ggaggggcca acgcctgcac gggctcgccc ggcttcctcg acacccatcg cacggccgag
     1381 aaggtcggcg aggtcctcgg cgtttccgcc ggcgacgtcg tggtctgctc gaccggtctc
     1441 atcggtgagc ggctgccgat ggatctgctc ctccccgggg tcgacgcggt cgccacggcc
     1501 ctgagcgtcg acgggcacga cgccgcggcg accgcgatca tgacgaccga caccgtctcc
     1561 aagcaggcga ccttcgtcgg cgacggctgg tcggtgagcg gcatggccaa gggcgccggc
     1621 atgctcgcgc ctgctctcgc aacgatgctc gtcgtcatca cgacagacgc cgtgacccac
     1681 ccgaaggaga tggacctcgt cctgcgcggg gcgacggccg tnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn ngcccgcgcg atcgcccgca acaacctctt caagtgtgcg
     1861 gtcttcggca acgaccccaa ctggggtcgg gtgctcgctg cggtcggtac cacgcgcgcc
     1921 gccttcgacc cagcccgcct cgcggtgacg atgaacggcg tccaggtatg ccgcgacggc
     1981 ggcgtggggg aggaccgctc gctggtcgac ctgacgccac ggcatacctc cgtgatcgtc
     2041 gacctgcacg aaggggaggc gcacgccacg gtgtggacca acgacctcac ccacgactac
     2101 gtccacgaga actccgccta cagcacctga tgtcgaagga ccctccgatg atgagctccc
     2161 tgcgtggtgt catcgacgcc gccgccgtgc gcgtcgccgc aagcaaggcc gccaccctcg
     2221 tcgaagccct gccgtggctg gaacgcttcc gtggggcgct ggtcgtcgtg aagtacggcg
     2281 gcaacgcgat gaccgacgac accctcaagg ccgccttcgc ccaggacatc gccttcctcc
     2341 ggtatgccgg cctgcatccg gtcgtcgtcc acggcggcgg cccgcagatc ggggcgatgc
     2401 tcaagcggat gggcctgacg agtgagttca agggcggcct gcgggtgacc acccccgagg
     2461 tcatggagat cgtgcgcatg gtgctcaccg gccaggtgca gcgcgagctc gtcggcctag
     2521 tcaatcagca tggcgcgctc gcggtcgggc tgtccggcga ggacgcccac ctgttcgagg
     2581 cgcgcaagcg cggcacggtc gtcgacggcg aggaggtcga cctcggcctc gtcggcgacg
     2641 tcgtgggggt caaccccgcc gcggtgctcg acatcatcgc ggccgggcgg atcccggtgg
     2701 tctcgaccgt cgcgcccgac ctcgacgagc cgggccaggt cctcaacgtc aacgccgaca
     2761 cggcggcggc ggcgctcgcg gtggcactgg aggcccgcaa actcgtcgtg ctcaccgacg
     2821 tcgagggcat ctatgccgac tggcccaacc gtgactcgct gctctcgcag gtcaccctct
     2881 cggaggcccg gctgcttctc gagcgcgtgg actcggggat ggtgcccaag ctcgaggcct
     2941 gcatccgggc ggtcgagcaa ggcgtgccgc aggcgcacgt catcgacggc cgcgccgcgc
     3001 actcggtgct gttggaggtc ttcaccgacg agggcatcgg cacgatggtc cttcccgatc
     3061 cgaccggaga ggtgtgggca tgaacgacct ggcaggcggg agcgcgaggg gcgacgcgag
     3121 cggcacctcc cacacgggcg agtggctcgg ccggtatgcc gcctcgctca tccccgtctt
     3181 cggcgtccca cagacggtcc tctcgcacgg gcaggggtgc tacgtcgtcg atgtcgacgg
     3241 caagcggtat ctcgacctgc tcgccgggat cgccgtcaac accctcgggc acgggcatcc
     3301 ggctctcgtc gatgccgtga cgaagcaggc atcccagctc gtccatgtct caaacttctt
     3361 caccagccag ccgcaggtcg agctggccga ggcgctnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnncgcg ctggccggtg acccgccgtg atccccgcaa cgccgaccgc
     3541 ccgtcgggcc aagatcgcgg ggctgctcgc cgaccgctcg gtgcgctcgc agggcgagtt
     3601 gctcacgctg ctcgccgacg cgggcatcga ggtcacccag gcgacgctct cgcgcgatct
     3661 ggaagcgctc ggcgcggtca aggtgcgcgc tggccgcggc cttgtgtatg ccgtccccgg
     3721 cgcctccccg tcgcccgtcg agcaggtgcc gtcccggctg cgccggttgt tggaggagtt
     3781 gctcgtcacc gctgacgcgt ccggcaacct cgtggtgttg cgcaccccgc cgggagccgc
     3841 caaccatctc gcgtcgacta tcgatgcgtc gcacctcacc gacgtcctgg gcaccatcgc
     3901 cggggatgac acgattctgt tggtcacgtc gtcggccaag ggcggccggg cggtcgcgac
     3961 gcggctgctc gccctggccg gggcgtcggc ctgacgtggc ggcggacctc atgcacgacc
     4021 agaatctccg tgcacccgtg agcgatccgg tgcacccatg aacacagaaa ggcccggtat
     4081 gccgcacgag gactccgctg gaacgagcgc ccgagtgagc ctgtggggtg gtcggttcgc
     4141 cggcggtccg gccgatgccc tcgccgaact ctcccgcagc acgcacttcg actggaggct
     4201 cgcggcatac gacattgccg ggtctcaggc acacgcgcgg gtgctgcatc gcgctggtct
     4261 gctgtccgac ggtgacctcg cgacgatgct tggggcgctg gacgagttgc tcgtcgacgt
     4321 ccacacgggg gcgttcgtgc cttcgccgga ggatgaagac gtgcacaccg cgctggagcg
     4381 cggcctgatc gagcgggctg gcgcggacgt cggcgggcgg ttgcgtgcgg ggcgctcgcg
     4441 caacgaccag atcgccactt tgctgcggat gtgtctgcgg gacgcggctc gtgacctcgc
     4501 tgcgcaggtg ctcgatctgg tcgatgcgtt ggtcggtcag gcgcagtctc atctcggggt
     4561 agcgatgccg gggcggacgc acatgcagca cgcgcaaccg gtgctgctgt cccatcatct
     4621 gctcgcccat gcgtgggcgc tgttgcgtga tgtcgatcgg ctgcgcgact gggaccgacg
     4681 cgccgactcc tcgccgtatg gctctggggc gttggcgggg agttcgctcg ggctggaccc
     4741 ggaggcggtg gcccgtgact tggggttctc gcggtcggtc gagaactcga tcgacgggac
     4801 ggcctcgcgc gacgtggcgg ccgaattcgc gttcgtgatg gcgatgacgg cggtcgacgt
     4861 gtcccggatc gccgaggaga tcgtcgtgtg ggcgaccaag gagttcggat acgtcacgct
     4921 cgacgacgcc tattcgacgg ggtcgagcat catgccgcag aagaagaacc ccgacatcgc
     4981 cgagttggct cgagggaagg ccggtcgggt cgtcggggac ctgactgggc tgctgacgac
     5041 gctcaagggc ttgccgctgg cgtacaaccg ggacctgcag gaggacaagg agccggtgtt
     5101 cgacgccatc gacaccttgg cggtgttgtt gccggccttc accgggatga tccagaccct
     5161 ggtcttcaac cgtgagcgtt tggaaagcct tgcgccccaa gggtttgccc tggccacgga
     5221 tatcgccgag tggttggtcc gtcagggggt gcccttccgg gtcgcccatg aggtggccgg
     5281 tgcgtgcgtg cgcgagtgcg agggccgggg gatcgagttg tgggagctga cggatgccga
     5341 gcttgcggcg gtgaatccgg ccctgacgcc ggcggtgcgt gaggtgctca gtgtggccgg
     5401 gtcgctggcc tcgcgggatg ctcgtggcgg cactgccccg gtccgggtga gcgagcaact
     5461 cgccgaagcc caagccgccg tcgccgccgc ccgagccttc gccgctcccg cctgaccctt
     5521 tgccgtcgcc cgcccccctt tgccgggtcg aggtattccg ccatcgtcag gtgatagcga
     5581 aatacctcga cccgctgttg gagctcggcg ggccggaaag atgaccgtgc gagcgctatc
     5641 tccgggtcga aacgaggctg ggggccggga agatgaccgt gcgagcgcta tctccggggt
     5701 gggtcagggc tagctgcgag gggagctgtg gaaggcgggg tcggcccgca gcgccatgaa
     5761 ctccagcgct cgctcctgag cggccaggtc gactgccgcc gtcggcgtcc acgtgggcac
     5821 gggacacggc cgcccatcgg catccagcgc gacatagacg ctcgtgcact gggttgccag
     5881 agccaactcg ccggtgcggg ggctgccggc caacacccgc agcgcgacat gcatcgaggt
     5941 ccgccccgtg tgcaccagcc gtgactggat ctcgacgaca tcgccgatcc ggatgggccg
     6001 caggaacgac accccgccgg agaaccgcga caccacgtgg gtctggctcc atcccgaggc
     6061 gcacacgtag ccggtctcat ccagccagcg catgaggatc ccgccatgca ccttgccgcc
     6121 ccagttaacg tcggtcggag cggcgaggaa gcgcagcgtc gccccgtgag ccagcgtcgc
     6181 tccctcccag tcctgcgcgg ccaccagtga cttgacccgc tcgcgcaggt cgatccgccg
     6241 ccgcgcctcg gcctgagcag cacgcagcac cgcgccgggc gggtcccatt gggccacgtc
     6301 aaccgtccgc ccctcgtcgt cgacggccac gaacaccatc cgacactcga acgcgtgctc
     6361 cacctgcccg ccgcgcgggt tccccgacga cacgtccacg acgatcgtca tgctcgaacg
     6421 cccagtgagg accagttgcg ccgacacctc caccaggtct cccacctgca ccggccggtg
     6481 gaagtcaatg ttccccacgt atgccgtcac gcaatagcgc cccgaccagc ctgcggcaca
     6541 agcgaacccg gccttgtcga tccattcgag gatccgacct ccgctcaccg acccgccttg
     6601 gcccgcgtca gtcggtgcgg cgaggaagcg cagggtgacc tggcccgtgg tggcggtgga
     6661 cggcataccg gagagtatgc cgcgcggctg cgcgagactg tcggcgtgtc ccgcctgccc
     6721 agtggcttct acgaccgacc ggtgctcgac gtggccgtcg acctgctcgg cgcgcgaatc
     6781 tcgcacggcg gcgtgacgat tcggctcacc gaggtcgagg cgtatgccgg ggtcggagac
     6841 ccgggatcgc atgccttccg aggtgagaca cctcgcacga cggtgatgtt cggagccccg
     6901 ggaggcttgt acgtctattt cacctacggc atgcactggt gcgccaacct ggtgtgtgga
     6961 ccggacggtc aggcttcggc ggtcctcatg cgcgccggcg aggttgttga cggagaggag
     7021 atcgcccgaa cccggcgcgg ggaggcttgt gcgacaaggg atctcgcccg tggcccggct
     7081 cggctcgcgt cagcgctcgg gctgaccggg gaagtcaacg gcacgctgac cttcgagccc
     7141 gacagcccgg tgagggtgca ggcagcggct gaggcgccag acccggcccg catccgcacg
     7201 ggaccccgcg tcggggtcag tggccccggg ggtgacggga cggcataccc ctggcggttc
     7261 tggatcgacg gcgaacccac ggtgtcggtc tatcgccccg gcaagccgcg acggcgcccc
     7321 tgacgcgggg gccgatcgac atcagcgcag gatcgcgtgc agcgtgcggc gcatgacatc
     7381 ggccagcgga tgccgccgcg gcgtgtaata gatgacgccg gccaccgcct gttgcagggc
     7441 gtagccgcgt gcccgcagcc acgagtcatc gtccgctgca acgccgtccc ggaagcgctc
     7501 gcgcccctgc ggaccgagaa cagcccaggc agggacgagg tcgagcgcgg ggtcgcccgc
     7561 ccccgcaccg ccccagtcga tcactgcggt gagccgattg ccttgcacga gaaggttgcc
     7621 cgggatgaga tcgccgtggc agaggacagc gtcaccgtcc cactcccgcg cggacaacga
     7681 ctcctgccac gacgcgcgca cgccgcgctc gtcgacgagg ccgtgcgcct cactcaacca
     7741 ctcgtcgatg tcctcggtca gcggggcgag cccgccccca cgtcgcccag gtgggcggac
     7801 ctcgacgatg tccccgtatg ccgcccacgg caccttcccg agggcgtcca ccaccgccgc
     7861 gaggtcctcc gcgagccgga agccgtgtgg atcgtcaagc gttgcccgat cggcccaggc
     7921 gtcccgtccg ggcaaccatc gcagcaccgc ccaggggtgg gggtagcgcg cgctcgcgga
     7981 accgaggtgg accagttcgg gaagagcgac tgggagccgc cctgcaaggt gggggagcag
     8041 ccgcgtttcg gtgcgcaggc tcgcggcggc tgacggggtg cgaggcagtc gcacgaccaa
     8101 ctccgttccg agccgataca gcacgttgtc cgacccggtg ttgccggcgg tgccgctgat
     8161 tcgcgcgagg gagagcgccg ccaggtgggg cgcctggctt cggagcaggt cgagcacgac
     8221 ctccgagccg gtgtcggctt cgtcgtcgtg cagcggttcc atcggctcat cccaccagtc
     8281 ggtggttccc gacgccagcg cgtttcct
//
LOCUS       DAORPW010000098         8306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2096_length_8306_cov_855.080960, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000098 DAORPW010000000
VERSION     DAORPW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2096_length_8306_cov_855.080960"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1311
                     /locus_tag="PLB86_08685"
     CDS             112..1311
                     /locus_tag="PLB86_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HRC65251.1"
                     /translation="MPFAHPRVRVPLRRSPVRRDPLRKNRFAIPGAVLAVALTASLSA
                     CSLSSTTTATAPATTSGTATASQVSSPSSTAAPRCGAVTEVLLVSHDSFGLSDELTAA
                     FTKATGCTIKVLASGDAGELTNKLALTKASPIGDAVFGIDNTFASRAVTEGVLADYSP
                     TLPAGASAHALPGAAATKLTPIDYGDVCVNVDHTWFAAKGKPVPATLDDLTQPAYKDL
                     FVTPGATTSSPGLAFLLATIGAKGDGWQAYWKALMANGTKITAGWTDAYSVDFSAGEG
                     HGTRPIVLSYASSPPFTIPKGGSEPTTKALLDTCFRQVEYAGVLAGARNPEGARALVS
                     FLLSPAVQASIPGSMYMFPVDSTATLPTDWAKWAKVADKPFVVSPDQIAAQRDTWLRQ
                     WSEVTAK"
     gene            1308..2972
                     /locus_tag="PLB86_08690"
     CDS             1308..2972
                     /locus_tag="PLB86_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRC65252.1"
                     /translation="MTRDRAGRIAATTVAAILPLGFLAIFFALPVAGMLARGFAPGGV
                     LDLGGFAEVLGRARTQRIIGFTLAISAAATVISVVVGVPVAHALYRLALPGRRTLRAI
                     VAMPFVLPTVVVGVMFRSLLAEGGALGGLGLDGEWGGILAAFVFFNLAVVVRTVGAAW
                     EGLDRRPEEAAGTLGAGPATVFRTVTLPALAPSIISAASIVFLFCATAFGVVLTMGGL
                     AYGTVETEIYLLTTALFDLRGAAVLSVVQLVAVVAMLLLADRARSRDAGTQRAAKSAI
                     APGRVTAYAMGSVIATVLAVAFVAVPVLALVVRSLRRQGEWTLDYYLALGRVGGADGR
                     SALPVTVWEALGTSWRIAIDATILAVVLGLLVAVLVSRPARSPGGLRWRRLLDGVVML
                     PLGVSAVTVGFGFLITLDRPPLDLRTSPVLIPIAQAIVALPLVVRTLAPVLRAIDDRQ
                     RQVAATLGARPWQVLATVDLPIIWRPLLAAVGLALAVSLGEFGATSFLARPDSPTLPV
                     VIYRLIGRPGVDNLGMALAASVILAVVTVTVMGVVERLRVSSVGAF"
     gene            2972..3631
                     /locus_tag="PLB86_08695"
                     /pseudo
     CDS             2972..3631
                     /locus_tag="PLB86_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3767..4357
                     /locus_tag="PLB86_08700"
     CDS             3767..4357
                     /locus_tag="PLB86_08700"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRC65253.1"
                     /translation="MRLLLIRHGQTASNVTRLLDTAAPGAPLDEVGLEQARALADALA
                     DEPIEAVYASDLTRSQQTAAPLAARHGVEVVVRGGIREIQAGEDEMSADWVRYLTTIM
                     SWNADLDARMPGGESGREALERFDAVLDEAHAAGHGTVAVISHGAMIRTWAAARATNL
                     TADFLRTTGLDNTIVLELTRTPQRTWTLVRWGDKTP"
     gene            complement(4365..4787)
                     /locus_tag="PLB86_08705"
     CDS             complement(4365..4787)
                     /locus_tag="PLB86_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65254.1"
                     /translation="MRVTMLLADHAVVAEGKLYVNGGGWSIRPAGPTPMFLALKFDVP
                     WDLAGRPIEVELRLVDEDGHLVAQRTPQGDQPVLVNARLEVQRPPGLTPGAPIDAAMA
                     IGVPPFGLPPGRRYSWRLSVDGHQREDWVIEFATRPEA"
     gene            4882..6096
                     /locus_tag="PLB86_08710"
     CDS             4882..6096
                     /locus_tag="PLB86_08710"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRC65255.1"
                     /translation="MRELTQSQKLADVCYDIRGPVLEEAKRLEDEGHRILKLNIGNPA
                     PFGFEAPDEILVDMIAQLPLAQGYSDSKGIIGARRAVTQYYRLKGVEGVSTEDVYLGN
                     GVSELIVMAMQAMLDNGDEVLIPAPDYPLWTAATALAGGTPVHYLCDESEGWVPDLAD
                     MAAKITPRTKAIVVINPNNPTGAVYPDHVLRGIIALAREHELVLMADEIYDKILYDDA
                     THTALASLAPDVFCLNFNGLSKAYRVAGFRSGWLALSGPKHHARSFIQGLNILANMRL
                     CANVPAQHAIATALGGYQSITELILPGGRLLAQRNLAWELINSIPGVSCTKPGGALYL
                     FPRLDPEVHEIHDDERFALDLVRQAKILVVQGTGFNWPRPDHFRIVTLPHVQELEEAI
                     GRIGNFLASYRQ"
     gene            complement(6176..6832)
                     /locus_tag="PLB86_08715"
     CDS             complement(6176..6832)
                     /locus_tag="PLB86_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HRC65256.1"
                     /translation="MSTDPTASEPSPLDPTAPLSSGYDPAATTPMSPPPPVTPPPAPV
                     GPWDSTPAAPPAPVAPPVAAAPPVPDYGQPAAYGQPAPYGQPAPYGQQAPYGQQPPYG
                     QQPGYGQPAPYASPYAAPPAYGGAAYAGIEHPQGTLILILGILSIVFCGFTGPFAWVM
                     GRKAMREIDTSGQPYSNRSQVQAGMITGIIGSVILVLGVLYFVFVFFIVGMAASSSIR
                     "
     gene            6878..7327
                     /locus_tag="PLB86_08720"
     CDS             6878..7327
                     /locus_tag="PLB86_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRC65257.1"
                     /translation="MTSTDPSLGLRSVRLERVAAGRYAVTNPRGGTVTLGRGQDSDYT
                     PVELLLAAIAGCTSVDVDEVTTRRSEPESFVVDCSGVKVSDNLGNHLSEVEVTFRISF
                     PDTESGAAAAALVPRLVSLSHEKLCTVSRTVMLPTPVRTVIADEGQE"
     gene            7324..>8306
                     /locus_tag="PLB86_08725"
     CDS             7324..>8306
                     /locus_tag="PLB86_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRC65258.1"
                     /translation="MSDVLGVLDPAAAPSLRWGIIAPGGIANKFAEAVSQFTAGQVVA
                     VSSRDEGRARSFAERHGIPRAYAGHEALGADPEVDAVYVASPHSAHREHAVAMARAGK
                     HVLIEKALARNLVEVNEIFDAARGAGVFAMEAMWTRHLPHIAAVRRWIADGRIGEVVT
                     VVADHGQSLDLGPDHRLKAPELAGGALLDLGVYPVAFVVDLLGAPTSVHAAGHRTETG
                     VDASVALTLGYPDPVFAQASCTLKVKTPTTAVVCGTGGRIELDGDFYGGTVARLLEPG
                     KVREVVEEFDGRVANGFQFEIAEVARCVADGRVESERMRWDDSRAVMSVLD"
BASE COUNT         1165 a         2925 c         2893 g         1323 t
ORIGIN      
        1 cgccgcagac cctcgaacct gctccggtta gcaccggcga agggagtcga gtctcacccg
       61 tcgaccttcg atgggtcgac ggcccctcct gaacaaggag gagatgtcag tatgccgttc
      121 gcccacccgc gcgtccgggt tccgttgcgc aggagtcccg tccgcaggga tcccttgcgc
      181 aagaaccgtt tcgcgatccc cggcgccgtt cttgcggtcg ccctcactgc gtcgctctcg
      241 gcctgttcgc tgagctcgac gaccaccgcc acagcgcccg caacgacgtc ggggaccgcg
      301 accgcgtccc aggtcagcag cccgtcgagc acggccgcac cccggtgcgg cgccgtgacc
      361 gaggtcctgc tggtcagcca cgactcgttc gggctgtccg acgagttgac cgcggccttc
      421 accaaggcga ccggctgcac gatcaaggtg ctggccagcg gcgacgcggg cgagttgacc
      481 aacaaactcg ccctcaccaa ggcctcgccg atcggcgacg ccgtcttcgg gatcgacaac
      541 accttcgcca gccgggccgt caccgagggt gtcctcgcgg actactcccc gaccctgccc
      601 gccggcgcgt cggcccacgc gctgcccggc gccgcagcca ccaaactgac gccgatcgac
      661 tacggcgacg tgtgcgtcaa cgtcgaccac acgtggttcg cggccaaggg caaaccggtc
      721 ccggcgaccc tcgacgacct cacccaaccg gcatacaagg acctcttcgt gaccccgggc
      781 gcgacgacct ccagccccgg tctggccttc ctgctcgcga ccatcggtgc gaaaggcgat
      841 ggctggcagg cctattggaa ggcgttgatg gccaacggca cgaagatcac cgccggttgg
      901 accgacgcct actcggtcga cttcagcgcc ggcgagggcc acgggacccg cccgatcgtc
      961 ctgtcctacg cctcctcgcc gccgttcacg atccccaagg gcggcagcga gccgacgacg
     1021 aaggcgctcc tggacacgtg cttccggcag gtggagtatg ccggtgtgct ggccggcgcc
     1081 cgcaaccccg agggggcgcg tgctctggtc tcgttcctgc tgagcccggc cgtgcaggcg
     1141 tcaatccccg gctcgatgta catgttcccc gtcgactcga ccgccaccct gccgaccgac
     1201 tgggcgaagt gggccaaggt cgcggacaag ccgttcgtcg tgtcgcccga ccagatcgcc
     1261 gcccagcggg acacctggtt gcgtcaatgg agtgaggtca cggccaagtg acccgggacc
     1321 gagccgggag gatcgccgcg accacggtcg cggcgatcct cccgctcggc ttcctcgcca
     1381 tcttcttcgc ccttccggtc gcaggcatgc tcgcccgggg cttcgcgccg ggcggggtcc
     1441 tcgacctcgg tggcttcgcc gaggtccttg gccgggcccg gacccaacgg atcatcggct
     1501 tcaccctggc catctcggcg gccgcgaccg tgatctcggt cgtcgtcggg gtgccggtgg
     1561 cccacgccct ctatcggttg gccctgcccg gccgtcggac gcttcgggcc atcgtcgcga
     1621 tgcccttcgt cctgccgacc gtcgtcgtcg gcgtgatgtt ccggtcgttg ctcgccgagg
     1681 gcggcgcgct cggtgggctg gggctggacg gggagtgggg cggcatactc gccgccttcg
     1741 tgttcttcaa tctcgcggtc gtcgtgcgca ccgtcggagc ggcctgggag ggcttggatc
     1801 gccggcccga ggaggccgcc ggcaccttgg gcgccggacc ggcaacggtc ttccgcaccg
     1861 tgacgctgcc cgccctggca ccgtcgatca tctcggcggc gtccatcgtg ttcctcttct
     1921 gcgccacggc ttttggtgtc gtgctgacga tgggcgggct ggcctacggc accgtcgaga
     1981 ccgagatcta cctgctgacg actgccctgt tcgacctgcg cggggcggcg gtgttgtcgg
     2041 tcgtccaact cgtcgccgtc gtcgccatgc tgctgctggc cgatcgggcc cgctcccgcg
     2101 acgccgggac ccagcgggcc gcgaagtccg cgatcgcgcc ggggcgggtc acggcatacg
     2161 cgatgggctc ggtgattgcc acggtgctgg cggtcgcgtt cgtcgcggtt cccgtgctgg
     2221 cgctggtggt gcgctcgctg cggcgccagg gggagtggac cctggactac tacctcgcgt
     2281 tgggacgggt cggcggggct gacggtcgta gcgcgctgcc ggtcaccgtg tgggaggcgc
     2341 tgggcacgtc gtggcggatc gccatcgatg ccacgatcct cgccgtcgtc ctcggcctgc
     2401 tcgtcgccgt cctcgtctcg cgcccggccc gctcgccggg cggtctgcga tggcgccggt
     2461 tgctcgacgg ggtggtcatg ctgccattgg gggtgtctgc ggtgacggtc ggtttcggct
     2521 tcctcatcac gttggaccgg ccgccgttgg acctgcggac ctcgccggtg ctcatcccga
     2581 tcgcccaggc gatcgtcgcc ttgcccctcg tggtgcggac ccttgcgccg gtgctgcggg
     2641 ctatcgacga ccggcaacgg caagtcgccg cgacgttggg tgctcgcccg tggcaggtgc
     2701 tcgccaccgt cgacctgccg atcatctggc ggccgttgct cgcggccgtc gggctggcgc
     2761 tcgcggtctc gctgggggag ttcggtgcga cgagcttcct ggcccgaccc gacagcccga
     2821 ccctccccgt ggtcatctat cgcctcatcg ggcggccggg ggtcgacaat ctcggcatgg
     2881 cactcgcggc gtcggtcatc ctcgccgttg tcaccgtcac cgtcatgggt gtcgtcgagc
     2941 ggttgcgggt gtcgtcagtg ggtgccttct gatgctctcg atcagccgtg tgtcggtgtc
     3001 tttcggggcg acccgcgcgg tcgacgatgt cagccttgag gtcccctcgg gcacggtgat
     3061 ggcgatcctt gggccgtcag ggtgcggcaa gtccagcctg ctgcgggccg tcgccgggct
     3121 cgaaccgctc gcgtcgggct cggtgagctt cgacggtgcg gatctggcgg gggtgcccac
     3181 ccatcggcgt ggcttcgcga tgatgttcca ggacgggcag ctgttcggtc acctcgatgt
     3241 ggccgggaac gtcggctatg ccttggcgcg caagggaatc cgcggatcgc aggcgcgaac
     3301 tgaggtcgac cgcctcctcg agctcgtcgg cctcgcgggg tatgccgcgc gccgccccgc
     3361 caccctcagc ggcggtgagg gtcagcgcgt cgcgttggcc cgggcgctgg ctgctcggcc
     3421 ccggctgctg ttgctcgatg agccgctgtc ggcgctggac cggtcgctgc gcgagcggct
     3481 ggccactgac ctgcggcacg tgctcaccga gactgggacg acggccatga tggtgaccca
     3541 cgaccatggc gatgccttca cggtggccga tcggacggcg atcatgcgag ccggacggtt
     3601 ggtgcagcaa ggtgagacgg ccgaggtctg ggcgcacccc atcgatgccg acaccgccca
     3661 cttcctcggc gtctcggtcc cctcggacct cgcggagccc tgagctcaga ccgctgcgtg
     3721 gcgataaccg cgccgatcgc aagccgggag cggctagcct cgatgggtgc gactgttgct
     3781 catccgacac ggccagaccg cgtcgaacgt cacccgactg ctcgacactg cggcccccgg
     3841 tgccccactc gacgaggtcg gcctcgagca ggcccgggcc ctggctgacg cgctcgccga
     3901 cgagccgatc gaggcggtct acgcctccga cctgacccgc agccagcaga cggccgcgcc
     3961 gctggccgct cgccacggtg tcgaggtcgt cgtccgtggc ggcatccggg agatccaggc
     4021 gggcgaggac gagatgtccg ccgactgggt ccgctacctc acgacgatca tgtcctggaa
     4081 cgcagatctc gacgctcgga tgccaggcgg ggagtcgggg cgggaggctc tagagcggtt
     4141 cgacgccgtt ctcgacgagg ctcacgctgc cggccacggg accgtcgccg tcatcagcca
     4201 cggcgctatg atccgcacgt gggccgcggc ccgcgcgacc aacctcacgg ccgacttcct
     4261 gcgcacgacc ggcctggaca acaccatcgt cctcgagttg acccggacac cgcagcggac
     4321 gtggaccctc gtgcgctggg gcgacaagac tccctgagcg tcccctacgc ctcggggcgg
     4381 gtggcgaact cgatgaccca gtcctcgcgc tggtggccgt cgactgacaa ccgccaggaa
     4441 taccgccgac cggggggcag cccgaagggc ggcacaccga tcgccatcgc ggcatcgatc
     4501 ggcgcaccgg gcgtgaggcc cgggggccgc tgcacctcca gccgggcatt gacgagcact
     4561 ggttggtcac cctgcggggt gcgctgggcg accaggtgcc cgtcctcgtc gaccaggcgt
     4621 agttccacct cgatgggacg accggccaga tcccacggca cgtcgaactt cagcgccagg
     4681 aacatcggtg tggggccggc cggacggatc gaccaaccgc ccccgttgac atagagcttg
     4741 ccttcggcga cgaccgcatg atcggccaac agcatcgtga cccgcatacc tgccagcatc
     4801 gcacgcctgg ctgaacccgg tctgctcacg tggtgagaaa cccgtgcggg ggcggcatac
     4861 cggccgtggg aggctggacc catgcgcgag ttgacccaga gccagaagct ggccgacgtc
     4921 tgttacgaca tccgggggcc ggtgctcgag gaggccaagc ggcttgagga cgagggccac
     4981 cgcatcctca agctcaacat cggcaacccg gcacccttcg gcttcgaagc ccccgacgag
     5041 atcctcgtcg acatgatcgc ccagctgccg ctggcccagg gctacagcga ctccaagggc
     5101 atcatcgggg cccgccgcgc cgtcacgcag tactaccggc tcaagggcgt cgagggcgtg
     5161 agcaccgagg acgtctacct cggcaacggc gtcagcgagc tcatcgtcat ggccatgcag
     5221 gccatgctcg acaacggcga cgaggtcctc atccccgcgc cggactaccc gctgtggacc
     5281 gccgcgaccg cgctcgctgg tggcaccccg gtgcactacc tgtgcgacga gagcgagggg
     5341 tgggtccccg acctggccga catggcggcc aagatcaccc cgcgcaccaa ggcgatcgtc
     5401 gtcatcaacc ccaacaaccc caccggcgcg gtctatcccg accatgtcct gcgcggcatc
     5461 atcgcgctgg cccgcgagca cgaactcgtc ctcatggccg acgagatcta cgacaagatc
     5521 ctctacgacg acgcaaccca cacggccctg gcgtccctgg cgcccgacgt cttctgcctc
     5581 aacttcaacg ggctgtccaa ggcctatcgg gtcgccggtt tccggtccgg ctggctggcg
     5641 ctctcgggcc ccaagcacca cgcccggtcc ttcatccagg gcctcaacat cctggcgaac
     5701 atgcggctat gcgccaatgt gcctgcgcag catgcgatcg ccacggcgct cggcggctac
     5761 cagtcgatca ccgagctgat cctccccggc ggccgcctgc tcgcccagcg caacctcgcc
     5821 tgggagctca tcaactcgat ccccggcgtc tcgtgcacca agcccggcgg cgccctctac
     5881 ctgttccccc ggctggaccc cgaggtccac gagatccacg acgacgagcg cttcgccctc
     5941 gatctggtcc gccaggccaa gatcctcgtc gtgcagggca ccggcttcaa ctggccgcgg
     6001 cccgaccact tccggatcgt cacgctgccg cacgtccaag agctcgaaga ggccatcgga
     6061 cgcatcggca acttcctggc cagctaccgg cagtagccga ttcgcgcacc aacgcgaggg
     6121 ggcggcatac cgactcggta tgccgccccc tcacgttcgc ggggttgagg gtgggtcagc
     6181 gaatgctgct gctggccgcc atcccgacga tgaagaagac gaaaacgaag tagaggactc
     6241 cgaggacgag gatgacggag ccaatgatgc cggtgatcat gccggcctgg acctggctgc
     6301 ggttgctgta gggctggccg ctggtgtcga tctcccgcat ggccttgcga cccatgaccc
     6361 aggcgaacgg cccggtgaat ccgcagaaga cgatcgacag gatgccgagg atgaggatca
     6421 aggtgccctg cgggtgctcg atgccggcgt aggcagcacc gccataggcg ggcggcgcgg
     6481 catacggaga ggcgtagggg gcgggctggc cgtaacccgg ctgctggcca tacggcggct
     6541 gttgcccgta gggagcctgc tggccgtaag gggcgggttg tccgtaagga gcgggttggc
     6601 cgtaggcggc gggttgcccg tagtcgggaa ccggcggggc tgcggcaacg ggcggggcga
     6661 cgggggcagg gggcgcggcc ggggtgctgt cccagggacc gacgggtgca gggggcggcg
     6721 tcaccggcgg gggcggggac atcggcgtgg tcgccgccgg atcgtatccg gaggagaggg
     6781 gggccgtggg gtcgagcggg ctgggctcgc tcgccgtcgg atcagtgctc atgaacacca
     6841 ccctacttgc ccccgttgcc cgtggcaggc tgtccccatg acctcgactg acccttccct
     6901 tggcctgcgt tcggtccgct tggagcgcgt cgccgccggc cggtatgccg tgaccaaccc
     6961 ccgcggcggc acggtcacgc tcggccgtgg ccaagactcc gactacacgc cggtggagtt
     7021 gttgctcgca gcgatcgccg gttgcacgag cgttgacgtc gatgaggtga ccacccggcg
     7081 cagcgagccg gagtccttcg tcgtggactg cagtggggtc aaggtctccg acaacctcgg
     7141 caaccacctg tcggaggtcg aggtgacctt ccgaatctcc ttcccggaca ccgagtccgg
     7201 ggcggctgcc gcggcgctcg tccctcggct ggtctcgctc tcgcacgaaa aactgtgcac
     7261 ggtcagccgg acggtcatgc tgcccacccc ggtgcgcacc gtcatcgccg acgagggcca
     7321 ggaatgagcg acgtgctggg cgtcctggac cccgctgctg caccgagcct gcgctggggc
     7381 atcatcgcgc cgggtggcat cgccaacaag ttcgccgagg cggtgagcca gttcactgcc
     7441 gggcaggtcg tcgcggtgtc ctcacgcgat gagggtcggg cgcgctcgtt cgcggagcga
     7501 cacggcatac cgagggcgta tgccgggcac gaagccctcg gtgccgaccc cgaagtcgac
     7561 gcggtctatg tcgcgtcgcc gcactcggcc caccgcgaac acgcggtcgc catggcgcgg
     7621 gccggcaagc acgtcctcat cgaaaaggcg ttggcgcgca acctcgttga ggtcaacgag
     7681 atcttcgacg cggcgcgcgg cgcgggcgtg tttgcgatgg aggcgatgtg gacccggcac
     7741 ctgccgcaca tcgccgccgt gcgtcgctgg atcgctgacg ggcggatcgg cgaggtcgtc
     7801 accgtcgtcg ccgaccacgg tcagagcctg gacctcgggc cggaccaccg gctcaaggca
     7861 cccgagttgg ccggcggcgc gctgcttgac ctcggggtct atccggtggc cttcgtggtg
     7921 gacctgctcg gtgcgccgac gagcgttcat gcggccgggc accgcaccga gaccggggtc
     7981 gacgcgagtg tggcgctgac gttgggctac cccgacccgg tgttcgcgca ggcgtcgtgc
     8041 acgctcaagg tcaagacgcc gacgacggcc gtggtctgtg ggaccggcgg gcggatcgag
     8101 ctcgatggcg acttctatgg cgggacggtg gctcgacttc ttgagcccgg caaggtccgc
     8161 gaggtcgtcg aggagttcga cgggcgggtg gccaacgggt tccagttcga gatcgccgag
     8221 gtcgcgcggt gcgtggcgga cgggcgggtc gagagcgagc ggatgcgctg ggatgactcg
     8281 cgcgccgtca tgagcgtgct cgacga
//
LOCUS       DAORPW010000099         8091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2192_length_8091_cov_699.627427, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000099 DAORPW010000000
VERSION     DAORPW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2192_length_8091_cov_699.627427"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1012
                     /locus_tag="PLB86_08730"
     CDS             2..1012
                     /locus_tag="PLB86_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC65259.1"
                     /translation="MAITPIDLADDALMSRMYAVVSAVRVFERPHFVSPPEASAIGDL
                     RHVDEGESTHLWGIWEGDALVGVITAWLPLSDNTDTVWMELDVHPGHRRRGHGTRAIE
                     TIVGFARANDRTRIVTEAHYPVDRADDHPYRLFAEHNGFRLGNTEMIRRLTLPVDPHL
                     LARLGDGARAAYQGRYRVEAFTEVPRALWPSLCECMNRVGTDAPTGEIDWEPETLTPE
                     RYGGYMELDRATGRVRLTAVAIDEASGEVVAFSELALPPTITRAQQWGTLVRADHRGH
                     RLGMAVKVANLVALQELYPERVDVTTGNANDNEWMVSINEQLGFRPLELCPAFYRTLD
                     PV"
     gene            1066..1368
                     /locus_tag="PLB86_08735"
     CDS             1066..1368
                     /locus_tag="PLB86_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HRC65260.1"
                     /translation="MLVAFSVAPSAPDHDGSYSRAIADVVRLVRASGLPQRTDSMFTT
                     IEGEWDECMAVVKGAVDLLLEHSPRVSLVLKADIRPGHTGELDGKVNRIEAVLSEG"
     gene            1481..4606
                     /locus_tag="PLB86_08740"
     CDS             1481..4606
                     /locus_tag="PLB86_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65261.1"
                     /translation="MTWAALRYRPAQALLLAVLTALVVGCAAFAPLYERALAQSLLRD
                     GLSRMPPAATAIELTTSREQGARVRAERVRDIFPTSLAGVYGPGVERWSGRVFVTGSQ
                     GVSTVTMWGSPTPCDGLVLDAGRCPQAPFEVLASTAEARLQGWAPGAVISAVEARGIA
                     SGPAFASPVTVVGTYTQEELAAGPWAGLRLGGRAGTVSAGLAATPLMDALVTGDGTFA
                     PDEQASSFVPGAPGWFQVAVSVTYPLNRDAISLESLDGIPAALSALGDAAARELPRIA
                     VSSAVGDLADELAEGRRQVLVIVPLLMAQLALLGVVVLGLVAESAVEQRRPEAALSRL
                     RGGGASGARRLLVAELGAAASVGAPLGLLGALGVTTLVRASWLAPGVPAELPWPTFAA
                     AGLGLILAWAAIIIAVGRVVREPVADLLRRVPSRQRGRRMGLVDAGVVGVSVAGVVAL
                     ASGNLAGPLALATPALVALAVGIVLAHLLTPAATLIGRRSLDRGRLTATLTAVQIARR
                     PAVRRIVTIITVAAALATFAVNALVVGQHNRELRARVETGAAVVLTTDSDDPAEVETA
                     LRQVDPAATKATPVAWIRQGSSDSITTMAVIPEEFARVADLAVDPAAFDLRPLQVAGT
                     LPPVITGTRLSVTIGSSAIEELDEVGDAPQRAGEGPIFAVRLGKPGGAEQTLSLDPIP
                     LTSSVPVVFTAEVDCPQGCSLLAIGVDRVGLDYRVLRGRFTITELRGDAGPPAAIGRP
                     DTWIGSGDKNADVLAPYAVPGDLGSPTAVALDLLSARTGVRVTAKGAVGNLPAFVVGS
                     LPPGTTSGGTFQGAGLDGIAVQFQPVGTLPYTPGGPSNVAIVSLRALLDRASTLAPVV
                     GRLQVYLADSSFEAPVRDALGAQGISVTTTVRESDRQALYDGSASAWGLRLAVVVGLL
                     AIVMAALVLVLVVTTAWRPRSRDYAALRMSGVPLAVLRRASALEQGTVVVVAGVAGIL
                     CGLLASRLALPMVPLFTRPSATFLADLRPAWSAVGGAAALVLGLLGIVAIVVGTRLVS
                     RAIPARLRERL"
     assembly_gap    4686..4785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4786..5286
                     /locus_tag="PLB86_08745"
     CDS             <4786..5286
                     /locus_tag="PLB86_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65262.1"
                     /translation="GARQHGHDVPHPSEVLDLVGILPLANAPLATLTPGQLQLAALAV
                     GIAAYPGVLLADEPTSQLDHAARDTVLAAIREVNAQVGTTVLIVTHDPQVAAALPRTV
                     TIRDGRVGGEGRSGQEYAVVSADGSLPLPPDALDRLPPGTLVRVHPESDGRWIIIPEE
                     DGHGPA"
     gene            5273..5989
                     /locus_tag="PLB86_08750"
     CDS             5273..5989
                     /locus_tag="PLB86_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC65263.1"
                     /translation="MGRHELTGAERGRSSAGRVERGPAQSVECRDVTVRFEPVTALAG
                     VSLRVQPGEFVAVTGPSGAGKSTLLWTLAGAVRPSEGQVTVGSTAIVSRDQAARLGVA
                     VVPQGNGLAAVLTALENVTLPLLAAGHGSAAARAAAADALAEVGLAESVGHLVEELSG
                     GQQQRVAVARGLALAGGVLLADEPTSELDHGNREIVLTLLRAYAAAGNVVVMATHDPE
                     AAAVADRVVRLDEGLLSVER"
     gene            complement(5999..6994)
                     /locus_tag="PLB86_08755"
     CDS             complement(5999..6994)
                     /locus_tag="PLB86_08755"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRC65264.1"
                     /translation="MEQRRWERLGRDLGVVGLGCWQLGAGWGAVSEDDAMATLHAAAD
                     AGVTCFDTADVYGDGRSERLVGRMRAERIADEEEPVFVATKMGRRANPHVPEAFTLEA
                     FRAWVDRSRANLDMDTLDLVQLHCPPPSVLDSDQVFDALDELVQEGRIAAYGVSVETC
                     AEALAAISRPGVVSVQIILNAFRPKPLEEVLPAAAAAGVAIIARVPLASGLLTGRYDE
                     STTFPAEDHRAFNRHGDAFDVGETFSGVPYEVGVQAAREIAALTPPGWSTAQLALRWV
                     LDQPGVSVVIPGARNPAQALDNVVAAHLDPLPQATLTAMAEIYDHHIREHIHARW"
     gene            7075..7608
                     /locus_tag="PLB86_08760"
     CDS             7075..7608
                     /locus_tag="PLB86_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC65265.1"
                     /translation="MPLAFDPIRRAASLWRARWGPDAQVSAMAAATSIMRVHQALLAE
                     FDGIVGRHGLTFARYEALVLLAFTRSGELPMSKVGERLMVHPTSATNIVQRLEAAGLV
                     ERRPNPSDGRGTLAAITETGRAVMEAATTDLVEAGFGLGMLDDADRDQLFGVLRRVRL
                     AKGDFLEGETEARSAES"
     gene            7685..>8091
                     /locus_tag="PLB86_08765"
     CDS             7685..>8091
                     /locus_tag="PLB86_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRC65266.1"
                     /translation="MEQTSGADRWQARYDKATARGQVRDADFTTLSGMPVEPVYGPGL
                     HGIPEPQTEAERERMERIGWPGEFPFTRGLYPTGYRGRTWTIRQFAGFGNAVQTNERY
                     KMILERGGGGLSVAFDMPTLMGRDSDDPHSLGEV"
BASE COUNT         1088 a         2810 c         2850 g         1243 t
ORIGIN      
        1 ggtggccatc acccccatcg acctcgccga tgacgcgctc atgtcccgca tgtatgccgt
       61 ggtctcggca gtgcgcgtct tcgagcgacc ccacttcgtg agtccgcccg aagcgtccgc
      121 gatcggcgat ctgcggcacg tcgacgaggg ggagagcacc cacctgtggg ggatctggga
      181 gggcgatgcg ctcgtcggtg tcatcacggc gtggctgccc ttgagcgaca acaccgacac
      241 cgtctggatg gagctcgacg tgcatccggg ccaccgccgg cggggccacg gcacccgggc
      301 gatcgagacg atagtcggct tcgcccgagc caacgaccgc acccgaatcg tcaccgaggc
      361 ccactaccca gtcgaccgtg ccgacgacca cccgtatcga ctcttcgccg agcacaacgg
      421 attccggctc ggcaacaccg agatgatccg acggctcacc ctgccggtcg acccgcacct
      481 gctggcccgg ctcggcgacg gtgcgcgggc ggcataccag gggcgttatc gggtggaggc
      541 gtttacggag gtgccgcgcg cgctgtggcc ctcgctgtgc gagtgcatga accgggtggg
      601 gaccgatgcc ccgaccggcg agatcgactg ggagcccgag acgctgaccc cggagcgcta
      661 cgggggatac atggaattgg accgcgcgac ggggcgggtg cggttgaccg cagtcgccat
      721 cgacgaggca agtggcgagg tcgtggcctt cagcgagttg gctctcccgc ccacgatcac
      781 ccgggcccaa cagtggggca cgctcgtgcg ggccgaccat cgcggtcatc gactgggcat
      841 ggccgtcaag gttgccaatc tcgttgcact gcaggagctt taccccgagc gtgtcgatgt
      901 cacgaccggc aacgccaacg acaacgagtg gatggtgtcg atcaacgaac agctggggtt
      961 ccggccgctg gagttatgcc cggcgttcta tcggacgctc gaccccgtct gatccggctc
     1021 gctccgccag ccccacgggc gcgcgacgcg gcatacggtg ggggcatgct cgtcgccttc
     1081 tccgtcgccc cgtccgctcc cgaccacgac ggctcctaca gccgcgcgat cgccgacgtc
     1141 gtccgactcg tgcgagcctc cgggctcccc caacgcaccg actcgatgtt caccacgatc
     1201 gagggcgagt gggacgagtg catggccgtg gtcaagggag ccgtcgacct gctgctggag
     1261 cactcaccga gggtgagcct ggtcctcaag gcggacatcc gccccggtca caccggcgaa
     1321 ttggacggca aggtcaaccg catcgaggcc gtgctctccg aaggctgacc tgcccctcag
     1381 tgtcgcgccc caccccggcc cgccgctgcg agcccagacc ggacggcata ccgtggtggg
     1441 ggagccgtca ccaggcgaac gagaggtcag ggggtgcggg atgacgtggg ctgcgttgcg
     1501 ctaccgtccc gcgcaagccc ttctcctcgc cgtgctcacc gcgctggtgg tgggctgcgc
     1561 ggcgttcgcg ccgctttacg aacgggcgct cgctcagtcg ctgctgcgcg acggcctgtc
     1621 ccgtatgccg cccgcggcga ccgcgatcga gctcaccacc tcgcgggagc agggggctcg
     1681 ggtgcgggcc gaacgggtgc gcgacatctt cccgacatcg ctggccgggg tctacggccc
     1741 gggggtcgag cgatggtccg gtcgggtctt cgtcaccggc tcgcagggcg tgtcgaccgt
     1801 gacgatgtgg ggaagtccga cgccgtgcga cgggctggtg ctcgacgctg ggcgttgccc
     1861 gcaggcgccg ttcgaggtgc tggcctcgac cgcagaggcg cgcctgcagg ggtgggcccc
     1921 cggagcggtg atctctgccg tcgaagcccg cgggattgcc agcggccccg ccttcgccag
     1981 cccggtgacg gtggtcggca cctatacgca ggaggaactg gccgcaggcc cctgggcggg
     2041 gcttcggctc ggtgggcggg ccgggaccgt gtcggcgggc ctggctgcga ccccgctcat
     2101 ggatgcactc gtcaccggcg acggcacctt cgcaccggac gagcaggcct cgtcgttcgt
     2161 cccgggcgca ccgggatggt tccaggtcgc cgtcagcgtg acctacccgt tgaaccgtga
     2221 cgcgatcagc ctcgagagcc tggacggcat cccggcagcg ctgagcgccc tgggggatgc
     2281 ggcagcgcga gagctacctc gaatcgccgt ctcgagtgcc gttggcgatc tggcggacga
     2341 gttggctgaa ggtcgccgcc aggtgctcgt catcgtcccg ttgctcatgg cccaacttgc
     2401 cctgctcggt gtcgtcgtgc tgggactggt ggcggagtct gcggtggagc agcgccgtcc
     2461 cgaggcagcg ttgagccgat tgcgcggagg tggggcttcg ggagcgcggc ggttgctcgt
     2521 cgccgaactc ggtgccgcag ccagcgtcgg tgcgcccctc ggattgctcg gcgctctcgg
     2581 agtcaccacc ctcgttcgag ccagctggct ggcccccgga gtcccggccg aactgccctg
     2641 gccgacgttc gcggccgcgg gtttgggcct gatcctggcc tgggcggcga tcatcatcgc
     2701 cgtcggaagg gtggtccgcg agcccgtggc ggacttgttg cgccgggtgc ccagtcgcca
     2761 gcgaggccgg cggatgggtc ttgtcgatgc cggcgtcgtc ggggtgtcgg tcgccggggt
     2821 ggtggcgctg gccagcggca acctcgccgg accgctcgcc ctcgcgaccc ctgccctggt
     2881 cgcgctggcc gtggggatcg tgctggccca cctgctcacc ccggccgcca ccctcatcgg
     2941 gcgacgatcg ctggaccgag gtcggctcac ggcgacgctt accgcggtcc agatcgctcg
     3001 acgtccagcg gtccggcgga tcgtcacgat catcaccgtg gcggcggcgc tcgcgacctt
     3061 tgcggtcaac gcactcgtcg tggggcagca caaccgggaa ctgcgcgcgc gggtggagac
     3121 cggggcggcc gtcgtcctga cgaccgactc cgatgacccg gccgaggtcg agacggcact
     3181 gcgtcaggtc gaccccgcgg caaccaaggc gaccccggtc gcgtggatcc ggcagggttc
     3241 ctccgactcg atcacgacga tggcggtcat ccccgaggag ttcgctcgag tggccgacct
     3301 ggcggtggac cccgctgcct tcgacctgcg ccccctccaa gtggccggca ccctcccccc
     3361 ggtgatcacc ggaacccgct tgtcggtgac catcggctcc agcgcgatcg aggagctcga
     3421 cgaggttggc gacgcacccc aacgcgccgg cgaggggccg atcttcgccg tccgactggg
     3481 caaacccggc ggagccgagc agacgttgag cctcgacccg atccccttga cgagcagcgt
     3541 gccggtcgtg ttcacggccg aggtcgactg tccccagggg tgctcgctgc tggccatcgg
     3601 ggtcgaccgg gtggggttgg actatcgggt cctgcggggc cgattcacca tcaccgaact
     3661 gcgtggagac gcgggtccgc cggcagctat cggccgcccc gacacgtgga tcggctcggg
     3721 cgacaagaac gccgacgtgc tcgctcctta cgcggtccca ggcgatctcg gcagccccac
     3781 cgccgtggcc ctcgacctgc ttagcgcccg cacgggcgtc cgggtcaccg ccaagggcgc
     3841 cgtcggaaac cttccggcgt tcgtcgtcgg ctcgttgcct ccgggcacca cctctggagg
     3901 gacgttccag ggggctggcc tggacgggat cgccgtgcag ttccagccgg tgggcacgct
     3961 gccctacacc ccgggcggcc cctccaacgt cgcgatcgtc agcttgcgcg cgctgctcga
     4021 ccgggccagc acccttgcgc ccgtcgtcgg tcggctgcag gtctatctcg ccgactccag
     4081 ctttgaggcg ccggtgcgcg atgcccttgg cgcccaaggg atctcggtca ccaccacggt
     4141 gcgagagagt gaccggcagg cgctctacga cgggtcggcg tcggcgtggg gcctgcgtct
     4201 ggccgtcgtc gtcggactgc ttgccatcgt catggccgcc ctcgtcctcg tcctggtcgt
     4261 gaccacggcc tggcgcccgc gctcgcggga ctacgccgcc ctgcgaatgt ccggcgtgcc
     4321 gcttgccgtg ctccgtcgtg caagcgccct cgaacagggg acggtcgtcg tggtcgccgg
     4381 ggtggccggc atactctgcg gactcctggc gtcgcgcctg gccctgccga tggtgccgtt
     4441 gttcacccgg ccttcggcga ccttcctggc cgacctgcgc ccggcgtggt ccgccgtcgg
     4501 cggggccgcg gcgctcgtcc tcgggctgct ggggatcgtc gccatcgtcg tgggcactcg
     4561 cttggtttct cgagccatcc cagcccgatt gagggaacgc ctgtgagcgc cccgacccgc
     4621 accggcctgg ccatcacgac ccacggcctc gttcacatct accacgcgga cgggcacgac
     4681 ctcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggagc tcgacagcat
     4801 ggccacgacg tccctcaccc gagcgaggtc ctcgacctgg ttggcatcct cccgctggcc
     4861 aatgccccgc tcgccacgct cactcccggg cagttgcagc tcgcggcgct cgcggtgggg
     4921 atcgcggcat acccgggggt gctccttgcc gacgagccga ccagccaact ggaccacgcc
     4981 gcccgcgaca ccgtgctggc cgcgatccgc gaggtgaacg cccaggtagg gaccaccgtc
     5041 ctcatcgtca cccatgaccc gcaggtggcg gccgcgctgc cgcgcacggt gacgatccgc
     5101 gacggtcggg tcggcgggga gggacgcagc ggccaggagt atgccgtggt gtcggcggac
     5161 gggtcgttgc cgctgccgcc cgacgccctc gaccgactgc cgccaggcac cctcgtgcgc
     5221 gtccatccgg agtctgacgg ccgctggatc atcatcccgg aggaggacgg ccatgggccg
     5281 gcatgagctg acgggcgccg aacgcgggcg ttcgtcggcc ggtcgagtgg aacgcgggcc
     5341 ggcgcagtcg gtggagtgcc gagacgtcac ggtccgcttc gagccggtca ccgcgttggc
     5401 gggagtgtcg ctgcgggtgc agccggggga gttcgttgcc gtcaccggtc cgtcgggagc
     5461 cgggaaatcg accctgctct ggaccctggc gggtgccgtg cgaccgagcg agggccaggt
     5521 gacagtgggg tcgacggcca tcgtgagccg cgaccaggct gcgcgtctcg gagtggccgt
     5581 cgtgccgcaa ggcaatggcc tggctgcggt gctcaccgct cttgagaacg tcactctgcc
     5641 gctgctcgcg gcgggccacg gatccgccgc ggcccgtgcc gccgctgctg acgcactggc
     5701 ggaggtcggt ttggccgagt cggtggggca cctcgtcgag gagttgtcag gcggtcagca
     5761 acagcgggtc gccgtggccc gcggactggc cctcgccgga ggagtgttgc tcgccgatga
     5821 gccgacgtcc gagctcgacc acggcaaccg cgagatcgtc ctgaccttgc tgcgggcgta
     5881 tgcggctgcc ggaaacgttg tcgtcatggc gacccacgac ccggaggcag cggccgtcgc
     5941 cgaccgggtc gtccgactcg acgaaggcct gctcagcgtc gaacggtgat gcgtgtgctc
     6001 accaacgggc gtggatgtgt tcgcggatgt ggtggtcgta gatctcggcc atggccgtca
     6061 gggtggcctg cggcaacggg tcgaggtggg cggcgacgac gttgtccaga gcttgcgccg
     6121 ggttgcgggc gcccgggatg accacggaca ctcccggctg atcgagcacc cagcgcagcg
     6181 cgagttgcgc cgtcgaccag ccgggtgggg tgagcgccgc gatctcgcga gccgcctgga
     6241 ccccgacctc gtagggcacg cctgagaagg tttcgccgac atcgaacgcg tccccgtgtc
     6301 ggttgaacgc gcggtggtcc tccgcgggga acgtggtcga ctcgtcgtaa cgcccggtca
     6361 gcagcccgct ggccagcggc acccgcgcga tgatcgcgac cccagcagcg gccgccgccg
     6421 gcaggacctc ctccagcggc ttgggccgga aggcgttgag gatgatttgc acactcacca
     6481 cgccgggtcg cgagatcgcg gcgagggctt ccgcgcaggt ctcgaccgac actccgtatg
     6541 ccgcgatccg cccctcctgc acgagctcgt cgagagcgtc gaaaacctgg tcgctgtcca
     6601 ggacgctcgg cggcgggcag tgcaactgga cgaggtcgag ggtgtccatg tcgaggttgg
     6661 cccgactacg gtcgacccat gcgcggaaag cctccagcgt gaacgcttcc ggcacatggg
     6721 gattggcacg tcgacccatc ttggtcgcca cgaagaccgg ctcctcctcg tcggcgatcc
     6781 gctcggcgcg catccggccg acaagtcgtt ccgagcgccc gtcgccatag acatcggcgg
     6841 tgtcgaagca ggtcactccc gcatcggctg ccgcgtgcaa ggtcgccatg gcgtcgtcct
     6901 cgctgaccgc gccccagccc gcgcccaact gccagcaacc caacccgaca acgccgaggt
     6961 cgcgtcctag ccgttcccac cgtcgctgtt ccatgggcgc caacggtatc cccgcggcat
     7021 acctgctgtt gggccgcctc ccggcgggtg gtccgggcgc gctagcctgc ggccgtgccc
     7081 ttggccttcg atcccatccg ccgcgccgcg tcgttgtggc gcgctcgatg ggggccggac
     7141 gcccaggtga gcgcgatggc ggccgcaacc tcgatcatgc gggtgcacca ggcgctgctc
     7201 gccgagttcg acgggatcgt cgggcggcac gggctgacct tcgcgcgcta cgaggcgttg
     7261 gtgctgctgg ccttcacgcg ttccggtgaa ctgccgatga gcaaggtcgg tgagcgcctc
     7321 atggtccacc cgacctcggc gaccaacatc gtccaacggc tggaggcagc cgggctggtc
     7381 gagcggcgcc ccaaccccag cgacgggcga ggcaccctgg cggcgatcac cgagacgggc
     7441 cgggcggtca tggaggctgc gaccacagac ctcgtcgagg ccggtttcgg gctcggcatg
     7501 ctcgacgacg ccgatcgtga ccagctcttc ggggtgctgc gtagggtccg gctcgccaag
     7561 ggcgacttcc tcgaaggcga gaccgaggcc agatccgctg agagctgagt gcggtgccgt
     7621 gacggacgtc gcaaagtagt aggacgtcct agtaaatctg ccatttcccg gttaccctgt
     7681 ccgcatggag cagacctcag gcgcggaccg ctggcaagcc cgctacgaca aggcaacggc
     7741 gcgcgggcag gtgcgcgacg cggacttcac caccttgtcc ggtatgccgg tcgagccggt
     7801 ctacgggccg gggctgcacg gcatacccga gccgcagacc gaggctgagc gggagcggat
     7861 ggagcggatc gggtggccgg gggagttccc cttcacccgc ggtctctacc ccacgggcta
     7921 tcgcgggcgg acgtggacga tccggcagtt cgccgggttc gggaacgcgg tccagaccaa
     7981 cgagcgctac aagatgatcc ttgagcgtgg cggcggcggg ctctcggtcg ccttcgacat
     8041 gccgaccctc atgggccgcg actccgacga cccgcacagc ctcggtgagg t
//
LOCUS       DAORPW010000100         8042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2205_length_8042_cov_788.733442, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000100 DAORPW010000000
VERSION     DAORPW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2205_length_8042_cov_788.733442"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1277
                     /locus_tag="PLB86_08770"
     CDS             33..1277
                     /locus_tag="PLB86_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC65267.1"
                     /translation="MNSAVARRRPGPQTRRAIERGAGTLSDAALRRIETQHDWYRALP
                     PAERSWVGLVAQAGIAAFVAWLADESGTAPVTADVFGTAPRELTSAITLRQTLDLIRS
                     VVDVVEDEVHVLAVPGDEQAVREGVLRYSREIAFAAAQVYAQAAEARGAWDARLEALV
                     VDAVLRGEADDSMQSRATALGWGSVSDVTVVAGRTPSGSSASAVDAIRRAAARRQVEA
                     LTAVQGRRLVIILGRTTRPAEIVTAVADLFGDGPIVLGPTVPHLFAAGRSARAAINGL
                     AAADGWPQAPRPVSADELLPERVLVGDVHARRVLVERIATPLRAHEVLLDTATAYLDS
                     GGLESAARVLFVHPNTVRYRLGKIAELTGYDLTHPRDGYAVRLALALARLSGSPPAAR
                     RPVPTPAVPRDGAAGDGIPQSL"
     gene            1357..2541
                     /locus_tag="PLB86_08775"
     CDS             1357..2541
                     /locus_tag="PLB86_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HRC65268.1"
                     /translation="MLAIVCPGQGSQAPGLLAPWLELPSVHDRMQWFTTVAGIDLLEH
                     GTESDADTIRDTAIAQPLIVASGLAALLELFPKPSEAFTQVSVGAGHSVGEITAAAGT
                     GVITAEQALVFVRERGRQMAKASAVTPTGMSAVVGGNAEEVAAAIAAQGLTAANMNTA
                     GQVVAAGTLEQLRALAAAPPAKARVIPLQVAGAFHTQHMAPAVDRLAAYARAMTTREA
                     HTKLISNRDGHVVRSGREVLNRLVTQVSSPVRWDLCMQTMLDLGVTGLIEIPPAGTLV
                     GLAKRAMPGVELLAIKGPEDLDAARRMVADHGDRPDPSADPEWSLVIAPTKGTLSFGS
                     VAVGDQISRGTVLAQVRTADGDVAVTAPHSGWLSQWLVRDGDDVGPGQPVLRLAPKGA
                     LS"
     gene            2538..3563
                     /locus_tag="PLB86_08780"
     CDS             2538..3563
                     /locus_tag="PLB86_08780"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRC65269.1"
                     /translation="MTVEALRPKGSGSIHPGTGASHARIWGLGGFRPERVVPNSELVE
                     AIDSSDEWIQERSGIKSRRWAGPDDTVVSMSEAAARDAIAAAGIEISQIEAIIVSTVT
                     HPYQTPAAAPIIADRLGLRCAAFDISAACAGYCHGISLANDMVRGGSAKFVLVIGVEK
                     LTDFTDLTDRGTAFIFADGAGAAVVGPSDFPGIGPTIWGSDGSQWETIRQTESWLDVR
                     DQGLGHPTIGMAGQSVFRWAVWSMAPVARQAIEAAGITPDDLDAFIPHQANMRIIDAM
                     LKQLKLPADIPVARDIAETANTSAASIPLATERMLREGEIPHGGLALQIGFGAGLVYA
                     AQVIVLP"
     gene            3639..3887
                     /locus_tag="PLB86_08785"
     CDS             3639..3887
                     /locus_tag="PLB86_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRC65270.1"
                     /translation="MAQSTQEILEGLAEIVNEETGIDTGSVELDKSFTDDLDIDSLSM
                     MTIVVNAEEKFNVRIPDDEVKNLKTVRDAVDFIAAAQG"
     gene            4038..5285
                     /gene="fabF"
                     /locus_tag="PLB86_08790"
     CDS             4038..5285
                     /gene="fabF"
                     /locus_tag="PLB86_08790"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRC65271.1"
                     /translation="MSSSDIRVVVTGLGATTPVGGTVPETWEAILAGRSGARTMDFDW
                     VAKYELPVTFAAQIHTPPDQVLAKVETRRLDPSSQYALIAAREAWADAGAPELDPERL
                     AVAVGSGIGGVWTLLSQWDTLREKGPRRVYPLSVPMLMPNGPSAAISLELGARAGAHT
                     PVSACASGAEGMGYAVQMIRSGRADVVVAGGTEAAIHPMPIAGFAAMQALSTRNDDPA
                     AASRPYDKDRDGFVLGEGAAIIVLESEAHARARGAKIYAELAGVGMSADAYHITAPEP
                     EGAGASRAMLEAVSTAGGTLRDVVHINAHATSTPVGDVAETNAIRRAFGDLADSIPVS
                     ATKSMTGHLLGAAGALEAIFTILALHHRLAPASINLDTMDPQISLDVVHGAHRQLPAG
                     DLLALDNSFGFGGHNVALAFRTV"
     gene            complement(5374..5889)
                     /locus_tag="PLB86_08795"
     CDS             complement(5374..5889)
                     /locus_tag="PLB86_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HRC65272.1"
                     /translation="MSVTASRNATRGVVFIHSTPTALCPHITWALENVLGQPLHLDWT
                     IQPAGPRMVRAEVAWTGDPGLGARLASALRGWDHVRYEVTEEPSPGVDGSRWSHTPTL
                     GLHHTWTSASGDAMVNEDRLRDVFETHRGDVPALHAAIDALLGAPWDRELEAFRYAGD
                     GAPVRWLHKVG"
     gene            complement(6085..6594)
                     /locus_tag="PLB86_08800"
     CDS             complement(6085..6594)
                     /locus_tag="PLB86_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65273.1"
                     /translation="MKKQTLVSAVAAVATAAVLGMGGAALAQAGQSVPTSVPTSVQAD
                     ASPSATATPGAPAGQGRERAPGLTKEKLKDWIAKHGLTGDTAVKVTEAAVAKEPTAYV
                     LRVGKLADGTYVARMMRPDGTRIVLMIDANFAVTSVEDAPERVPGQGRGAGKGAKNNA
                     STASPTASS"
     gene            6908..6981
                     /locus_tag="PLB86_08805"
     tRNA            6908..6981
                     /locus_tag="PLB86_08805"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6942..6944,aa:Ile,seq:cat)
     gene            complement(7088..>8042)
                     /locus_tag="PLB86_08810"
     CDS             complement(7088..>8042)
                     /locus_tag="PLB86_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRC65274.1"
                     /translation="VTCATTPPPASVKFAAVGDIGTDSENGSTAASSMLKGLGANGYN
                     FALNLGDLGYNNNPGVEQNYCTFLKNNAGSSLQWLMVPGNHEGTDPTYGGPEGDYRKY
                     VAYCAGSTPPAGVVGNYPYDYYVDRGNVRIIMLSPNISNPTGKLTYRNGTAEQAKLGT
                     WIKGAKSAGKFVVVGAHEPYLTIGAHRLTPNNLADPQFAAFLVTQGVDLSLSGHDHNI
                     SRAYQIGGPVTSQTSPTVVDRDGSFVSGAGTVFAVLGTGGHSPREIGSLGSIWAMGSG
                     TNSPGGISFGFGEITVSGNTLTYQLRTVSGPKLTDVFTIIR"
BASE COUNT         1216 a         2876 c         2649 g         1301 t
ORIGIN      
        1 ggttgtgggc ggcatacaaa taggatgctg gcgtgaacag cgccgttgcc cgtcgccgcc
       61 cggggccaca gacccggcgc gcgatcgagc gtggggccgg caccctctcg gatgccgcat
      121 tacgcaggat cgagacccag cacgactggt accgcgcgct cccgcctgcc gaacgctcgt
      181 gggtcggtct ggtcgcccag gccgggatcg ccgccttcgt cgcgtggctg gctgacgagt
      241 cgggcaccgc gccggtgacc gctgacgtct tcggcacggc acctcgggag ctcacgtcgg
      301 ccatcacctt gcgccagact ctcgacctca tccgcagcgt cgtcgacgtc gtcgaggacg
      361 aggtccatgt gttggccgtg ccgggcgacg agcaggcggt gcgcgagggg gtgctgcgct
      421 actccaggga gatcgccttc gctgcagcgc aggtctatgc ccaagccgcc gaggctcgcg
      481 gcgcgtggga cgcccggctg gaagcactcg tcgtcgacgc ggtcctgcgc ggcgaggccg
      541 acgactcgat gcagtcgcgc gccacggccc tgggctgggg ctcggtgtcg gacgtcaccg
      601 tcgtcgcggg ccgaactcct tctggcagta gtgcatccgc ggtcgatgcc atccgtcgag
      661 ccgccgcgcg ccggcaggtc gaagcgctca ccgcggtcca aggtcgacgt ctggtcatca
      721 tcctcgggcg cacgacccgc ccggccgaga tcgtcaccgc agtggccgac ctcttcggcg
      781 acggcccgat cgttctcggc ccgacggtgc cccacctctt cgccgccgga cgctctgcgc
      841 gcgccgccat caacggtctc gccgctgccg atggatggcc gcaggcgccc cgaccggtgt
      901 cggccgacga gctcctcccg gagcgcgtcc tcgtcggtga cgttcacgcc cgacgggtgc
      961 tcgtcgagcg gatcgcgacg ccgttgcgcg cccacgaggt gctgctcgac acggccacgg
     1021 catacctcga ctccgggggt ctcgagtcgg cggcgcgggt cctcttcgtc catcccaaca
     1081 ccgtgcgcta ccgcctcggg aagatcgccg aactcaccgg ttacgacctc acccaccccc
     1141 gcgacgggta tgccgtccgc ctggctctcg ccctcgcccg cctctccggg tcccctccgg
     1201 cggcccgccg tccggttccg acgcccgcgg tgccccgcga cggagcggcc ggagacggca
     1261 taccccaatc tttgtaggaa acctccaaac ctgccccccg gaacttcgtc ggtttcaggt
     1321 cacgagaaga tcacggcttc gtgacacgct ggtgacgtgc tcgcgatcgt ctgccccggc
     1381 cagggctcac aggccccagg actgctcgct ccgtggttgg agctgccctc cgtccacgac
     1441 cggatgcagt ggttcaccac cgtcgctggc atcgacctgc tcgaacacgg caccgagtcc
     1501 gacgccgaca cgatccggga cacggcgatc gcccaaccgc tgatcgttgc gtccggcctc
     1561 gccgcgctgc ttgagctctt cccgaagccc tcagaggcgt tcacccaggt gtcggtcgga
     1621 gccgggcact ccgtcggcga gatcacggcc gcggcaggga ccggggtcat caccgccgag
     1681 caggcgctgg tgttcgtccg tgagcgcggt cgccagatgg cgaaggcgag cgcggtgact
     1741 ccgacgggga tgagcgcggt cgtcggcggg aacgccgaag aggtcgcggc ggccattgcc
     1801 gcccaggggc tcaccgccgc caacatgaac accgccggcc aggtcgtcgc cgctgggacg
     1861 ctcgagcaat tgcgggccct cgccgccgcg ccaccggcca aggcccgcgt catcccgttg
     1921 caggtggcgg gcgcgttcca cacccaacac atggcccccg ccgtggatcg gctcgcggcc
     1981 tacgcccgcg cgatgaccac ccgcgaggcc cacaccaagc tcatctccaa ccgcgacggg
     2041 cacgtcgtgc gctcgggccg cgaggtgctc aaccgcctcg tgacccaggt cagctcgccg
     2101 gtgcgttggg acctgtgcat gcagacgatg ctcgacctcg gcgtcaccgg cctcatcgag
     2161 atcccgcctg ccggcaccct cgtcggtctg gcgaagcggg ccatgcccgg ggtcgagttg
     2221 ctggcgatca aggggcccga agacctcgac gcggcccggc gcatggtcgc cgaccatggc
     2281 gatcgcccgg acccgtcggc cgaccccgag tggagcctgg tcatcgcccc gaccaagggc
     2341 acgctgagct tcggttcggt cgccgtcggc gaccagatca gccggggcac cgtcctggcg
     2401 caggtgcgca ccgcggacgg cgacgtcgca gtgaccgcgc cgcacagcgg ctggctgagc
     2461 caatggctcg tccgcgatgg agatgacgtc ggcccgggcc aaccggtcct gcggttggcc
     2521 ccgaagggag cactgtcgtg actgtcgagg cacttcggcc caagggctcc ggctcgatac
     2581 acccgggaac gggtgcgagc cacgcccgca tctggggcct cggcgggttc cggcccgagc
     2641 gggtcgtgcc caactccgag ctcgtggagg ccatcgactc ctccgacgag tggatccagg
     2701 agcggtccgg catcaagtca cggcgttggg ccggtccgga cgacaccgtg gtgtcgatgt
     2761 ccgaggcggc cgcgcgtgat gccattgcgg ctgcaggcat cgagatctcc cagatcgagg
     2821 cgatcatcgt ctcgacggtg acccacccct atcagacccc ggcggcggcg ccgatcatcg
     2881 ccgaccggct cgggctgcgc tgcgccgcct tcgacatctc ggccgcctgc gccggctatt
     2941 gccacggcat ctcgttggcc aacgacatgg tccgcggcgg cagtgcgaag ttcgtcctcg
     3001 tcatcggcgt cgagaagctc accgacttca ccgatctcac cgaccgcgga acggcgttca
     3061 tcttcgccga cggcgccggt gcggccgtgg tcggcccctc ggacttcccg ggtatcggcc
     3121 cgacgatctg gggatcggac ggctcgcagt gggagaccat ccgccagacc gagtcgtggc
     3181 tggacgtgcg cgaccagggc ctggggcacc ccaccatcgg catggccggt cagtcggtgt
     3241 tccgctgggc cgtgtggagc atggcaccgg tggcccggca ggcgatcgag gccgccggca
     3301 tcaccccgga cgacctggac gccttcatcc cccaccaggc caacatgcgg atcatcgacg
     3361 cgatgctcaa gcagctcaag ctcccggccg acatccccgt cgcccgcgac atcgccgaaa
     3421 ccgccaacac ctcggcggca tcgatccctc tggccaccga gcggatgctg cgcgaaggtg
     3481 agatcccgca cggcggcctc gctctgcaga tcggcttcgg ggccgggctg gtgtatgccg
     3541 cgcaggtcat cgtcctgccc tgacagattc ccgcgcctcg cgggtcaacg aggcacccag
     3601 atcctcgaaa agttttgtcc acgaaggaga tacacaccat ggcacagagc acccaggaga
     3661 tcctcgaagg tctcgccgag atcgtcaacg aagagaccgg gatcgacacc ggctcggtcg
     3721 agttggacaa gtcgttcacc gacgacctgg acatcgactc gctgtcgatg atgacgatcg
     3781 tcgtcaacgc cgaggagaag ttcaacgtgc gcatcccgga cgacgaggtc aagaacctca
     3841 agaccgtgcg cgacgctgtc gacttcatcg cggccgccca gggctgagcc ccacccggtc
     3901 gagcgctgcc acgcgatcga ccagcgccac cgcttgtggc gggaccccgc tggaaccgca
     3961 gccggggcac ccgccgcacg cggacggcat acccaatgaa tcacgtgctg tccccacgaa
     4021 ccaggagccg aaaccccatg tcctccagcg acattcgcgt cgtcgtcacc ggcctcgggg
     4081 ccaccacgcc cgtgggcggc accgtcccag agacctggga ggccatcctc gccggtcgct
     4141 cgggcgcccg gaccatggac ttcgactggg tggccaaata cgaactgccg gtgaccttcg
     4201 ccgcccagat ccacactccc ccggaccagg tgctggccaa ggtggagacc cgacggctcg
     4261 acccgtcgtc gcagtacgcc ctcatcgccg cccgcgaggc ctgggccgac gcaggtgccc
     4321 ccgagctcga ccctgagcgg ctggccgtcg cggtcggctc cgggatcggc ggcgtgtgga
     4381 ccctgctgtc gcagtgggac acgctgcgcg agaagggccc ccgccgcgtc tacccgctgt
     4441 cggtccccat gctcatgccc aacggacctt ccgcggcgat ctcgctcgag ctcggcgcgc
     4501 gcgccggtgc gcacactccc gtcagcgcct gtgcgtccgg tgccgagggc atggggtatg
     4561 ccgtgcagat gatccgcagc ggccgcgccg acgtggttgt cgccggcggg accgaagcgg
     4621 cgatccaccc gatgccgatc gccggtttcg ctgcgatgca ggccctttcc actcgcaacg
     4681 acgaccccgc cgccgcctca cgcccctacg acaaggaccg tgacggcttc gtgctcggcg
     4741 agggcgcggc gatcatcgtc ctggagtccg aagcacacgc gcgggcccgc ggcgccaaga
     4801 tctatgccga gttggcaggg gtcgggatga gcgctgacgc ttaccacatc accgctcccg
     4861 agcccgaggg cgccggcgcc tcccgcgcca tgctggaggc cgtgtcgacc gccgggggca
     4921 ccctgcgcga tgtcgtccac atcaacgccc acgcgacctc gaccccagtc ggcgacgtgg
     4981 ccgagaccaa cgcgatccgt cgcgccttcg gcgacctggc cgactccatc cccgtcagtg
     5041 ccaccaagtc gatgaccgga cacctgctcg gtgccgccgg ggcgctggag gccatcttca
     5101 cgatcctcgc cctgcaccac cggctcgccc ccgcctcgat caacctcgac acgatggacc
     5161 cgcagatctc cctcgacgtc gtccacggcg cgcaccggca gttgccggcg ggtgacctgt
     5221 tggccttgga caactcgttc ggcttcggcg ggcacaacgt ggccctcgcc ttccgcaccg
     5281 tctgaggccc ctcgatcgcc caccgcccag cgagcctttg gctgctgggc ggtggcgcct
     5341 tgccggagcc gccgccagag cgggtgaccc cggtcagccc accttgtgca gccagcgcac
     5401 gggagccccg tcaccggcat accggaatgc ctccaactcg cgatcccacg gggcgccgag
     5461 gagcgcgtcg atggccgcat gcagcgcggg gacgtccccg cggtgggtct cgaagacatc
     5521 gcgcagccgg tcctcgttga ccatggcgtc gccgctggcc gaggtccagg tgtgatgcag
     5581 cccgagggtg ggggtgtgcg accagcgtga gccgtcgacg ccgggagagg gctcctcggt
     5641 cacttcgtag cgcacgtggt cccagccacg cagcgcgctg gccagccggg cgccaaggcc
     5701 cgggtcaccc gtccaggcga cctcggctcg gaccatccgc ggtccagccg gttgaatcgt
     5761 ccagtcgagg tgcaggggtt ggccgaggac gttctccagg gcccaggtga tatgcgggca
     5821 caacgcagtc ggggtcgagt gaatgaacac gaccccgcgc gtcgcgttcc gggacgcggt
     5881 gacagacatc cggtactcct cacgtgcgag ggacgtcttc cccaacgtct cgctcgatcg
     5941 gtatgccgtg gtgctggctt gcgctcggag ggtgtcgctc acttggtggt gcgacacgcg
     6001 tcacgtgagt cacattctgc cccaaacaca tctcccgcac cagcgcggcc tggtgcggga
     6061 gatgtgtgtg gagttctaga gcggttagct cgatgcggtc gggctcgcgg tcgacgcgtt
     6121 gttcttggcg cccttgcccg ccccccgacc ctgtccggga acccgctccg gagcgtcctc
     6181 gaccgacgtc acggcgaagt tggcgtcgat catcaggacg atccgggtcc cgtccggtcg
     6241 catcattcgc gccacgtagg tcccgtcagc taacttgccg acccgcagca cgtaggccgt
     6301 cggctccttg gcgacggctg cctccgtgac cttgacggcg gtgtcgccgg tgagaccgtg
     6361 cttggcgatc cagtccttga gcttctcctt ggtcaagccg ggggcacgct cgcgcccttg
     6421 gcccgcgggt gctcccggcg tcgcggtcgc cgaggggctt gcgtcggcct gcaccgacgt
     6481 ggggacagag gtgggcaccg actggcccgc ctgggcgagc gcggcgccgc ccatgcccag
     6541 cactgccgcg gtcgccactg cggcgactgc gctcacgaga gtctgcttct tcactgacgg
     6601 ctcctttgtg tggcgtctgg tgtccacatc atgcgaacag ccgtgttacg gcccgtcgtg
     6661 acaatcctgt caagaacctg tgactcgctc tgcgcggcat gaagcgcatg gcactgtcct
     6721 cctcgtcgat ggtctcgcgt cagggtgacc gtctaagcgg ttgtgaacgg gtgtcgcttc
     6781 catgggcagg cggcggtgag tccctgacct ccccctggga cgggtaggct cgcgtccgca
     6841 cgaacgaccc cctcatcgat atgccgcccg cgcgctgagc gtcggatggc caggccccga
     6901 ggggtgcggg gcggtagctc agccggtcag agcagcggac tcataatccg tcggtcgtgg
     6961 gttcaagccc cacccgcccc acgatataac cgcaggtcag aggtatgacg gccgattgca
     7021 cgtccgtccg ccaccgcttc gcttagcatg accctgcgat accgagagcg ccgcagggtc
     7081 acacgcatca tcgaatgatc gtgaagacgt ccgtcagctt cgggccgctg acagtccgga
     7141 gttgatacgt gagggtgttg ccactcacgg tgatctcacc gaacccgaac gagatacctc
     7201 caggactgtt ggtccccgag cccatggccc agatggagcc caaagagccg atctcccttg
     7261 gcgagtgccc accagtgccg aggacggcga atacagtccc tgcgccggag acgaacgatc
     7321 cgtccctgtc cacgactgtg ggagaggtct gcgatgtgac gggccctccg atctgatagg
     7381 cacggctgat gttgtggtcg tgcccagaca gtgacaggtc aaccccctgc gttaccagga
     7441 acgccgcgaa ctgagggtct gcaaggttgt taggggtcag acggtgggcg ccgatggtca
     7501 agtatggttc atgggcacca acgaccacga acttgccagc cgattttgcc cccttaatcc
     7561 aggtgcccag tttggcctgt tcggccgtac cattacggta cgtcagcttc cctgtcggat
     7621 tgctgatatt tggggagagc atgatgattc tgacgttccc ccggtctacg tagtagtcgt
     7681 acgggtaatt tccgacaaca ccggctggtg gggttgatcc tgcacagtag gcgacgtact
     7741 tcctgtagtc gccctccggg ccgccgtatg tcgggtcagt tccctcgtgg ttgccgggga
     7801 ccatcagcca ttgaagactg ctaccggcat tgttcttgag gaacgtgcag tagttctgct
     7861 cgaccccggg gttgttgttg tacccgagat cccccaggtt cagtgcgaaa ttgtagccgt
     7921 tggctcctag tcctttgagc atggaactcg cggcagtgga accattctcc gagtcggtcc
     7981 cgatatctcc cacagcggcg aatttcacgc tcgcaggagg tggcgttgtc gcgcatgtga
     8041 cg
//
LOCUS       DAORPW010000101         7948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2251_length_7948_cov_716.930318, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000101 DAORPW010000000
VERSION     DAORPW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2251_length_7948_cov_716.930318"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLB86_08815"
     CDS             complement(<1..681)
                     /locus_tag="PLB86_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC65275.1"
                     /translation="MTTTTLSPPSGQPSSPGLPLEVRGLTKNFGSLVAVDHLDFSVEP
                     GRVTGFLGPNGAGKTTTLRMLLGLVRPTSGTATIGGSTYHDLARPLRTVGAALESSSF
                     HPGRSGRDHLRVLAAAGGIPTQRVDELLELVGIPAAARQRAGGYSMGMRQRLALAAAL
                     LGDPQVLLLDEPANGLDPEGIRWLRQFLRHLAAQGKTILVSSHMLSEVEQTVDDVVII
                     ANGRSVAQG"
     gene            complement(795..>1844)
                     /locus_tag="PLB86_08820"
     CDS             complement(795..>1844)
                     /locus_tag="PLB86_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRC65276.1"
                     /translation="DVPRVATWLRENATDARGLDGIPEVGQFGGGASNLTYLLRYAAS
                     PTAPAGRDLVLRRPPAGTKAKGAHDMRREHDLQAALAPVFPLVPRMVACCQDHEVLGS
                     DFYVMERVAGIILRRNLPAELGLDADTTRRLCVAAVDTLADLHAVDVDAARLAGLGRG
                     PGYVGRQVAGWSERYQKARTPDVPDAEAVMAWLAAEQPPDVATVLIHNDFRFDNLILD
                     PTDPTRIVGVLDWELATVGDPLMDLGSALAYWVQADDDLVMQRMRRQPTHAPGMLTRA
                     EVVERYTERAGLVVSPQQWRFYEVFGLFRLAVIAQQIYYRFVHGQTTNPAFAEFGAAV
                     AYLDYRSRSILESRG"
     assembly_gap    1845..1944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1945..3158)
                     /locus_tag="PLB86_08825"
     CDS             complement(<1945..3158)
                     /locus_tag="PLB86_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC65277.1"
                     /translation="MDFTPSPRSADLTARVRDFIDSRIDPVEATILADVQARRDRGEN
                     PWTVHPLIPELQALARTQGLWNLFLPAGHEGPYAARFGTDGGVGLSNVDYAPIAEQTG
                     RSSLAPLVFNCNAPDTGNMEVLLKYGSQEQKDTWLEPLLDGRIRSAFLMTEPDVASSD
                     ATTMAATARIEGDEVVLDGRKWWSTGVGHPDCAVGIFMGVTDPQAPKHARHSMVLVPL
                     DAPGVRIERVMPALGQWDEPLGHGEVSLTDVRLPASAIIGGPGRAFVIAQGRLGPGRV
                     HHCMRLIGLAERSLESACERASTRVAFGKPLANLGGNRERLAEARIAINQARLHVLHC
                     AWKLDAFGVEGAASEVSEIKAAVPRMACQVIDLAIQLHGGAGVSADTPLAAAYATARA
                     LRLADGPDEVHLG"
     gene            3253..4713
                     /locus_tag="PLB86_08830"
     CDS             3253..4713
                     /locus_tag="PLB86_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRC65278.1"
                     /translation="MGEWLLVLLAVILTVGTAIFVATEFALVALDRPTVQQAIDAGDT
                     RARGVLASLRRLSTQLSAAQVGITVTTLLVGYLAQPSIGHLLEAPLAAVLPAGSVESV
                     AAMLALVLVTLFSMVFGELVPQFLGISAPLPTAKVVAAPVRWFSMLAKPLIWVLNGSA
                     NGFLRGMGIEPQEELSGARTPQELASLVRRSALVGTMERETAERVTRTLDFGDRTAAD
                     VMTSRVRAVSIDRTASAADVVALARQTGHSRFPVVGDDWDDVDGIVHVKRALAVPPER
                     RVLVPVSALMVAHVTVPETIRLDALLVQLRQSGLQLAVVVDEYGGTAGVVTLEDVVEE
                     IVGDISDEHDWSGVSGRRAKDGSWSVPGTWRPDEVRSRLGAPVPDHAAYATLGGFLMA
                     ALGRVPERGDTVSLPGWKVRVQRMEGRRVARVRLVPVTPAVTDDPANATGREGDAGAP
                     GASRGPGVQRDPGSPRAPRGPLQPSQPASPPGEVTG"
     gene            4710..5753
                     /locus_tag="PLB86_08835"
     CDS             4710..5753
                     /locus_tag="PLB86_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRC65279.1"
                     /translation="MSHSMALWISLGLLLANAFFVGAEFAAMAARRSALEPLAAAGSA
                     RAATCLAALEQMGSMLATAQLGITVCSVGLGALAEAALHEVLHPVFAALPLGEAWANG
                     LSFAGALLIVVYLHVVAGEMIPKNLALAGPERAALVLVPALLMVSRVLRPVVAVMEWV
                     AKGLVRAIFRIEPKDEIASAFTVEEVTHILAESEREGLIEEHREGLVRSALEFSAKLA
                     ADVAVPVDRLVTVAKGVTPEDVERLVARTGFSRYPFLDKEGHVAGYLHLKDILYAADA
                     AARTERVPRKRLRRLATVAPTEEIEDVLATMRGNGTHLARVVDPTGEVIGVIFLEDIV
                     EELVGTISDAETR"
     gene            complement(5835..5906)
                     /locus_tag="PLB86_08840"
     tRNA            complement(5835..5906)
                     /locus_tag="PLB86_08840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5871..5873),aa:Arg,seq:ccg)
     gene            5999..6439
                     /locus_tag="PLB86_08845"
     CDS             5999..6439
                     /locus_tag="PLB86_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC65280.1"
                     /translation="MEPTLGAGWGGNGVVLVCDDTESIRRLLRINLELDGFEVLEACD
                     GASAIALLRALQLEDRLPDVILLDAHMAPLDGWWALSEIRSCPRLSRIPVVMATADTV
                     CEDKAAMLARGLDACVQKPFDPEAVVDLVAGFVREGRAHVPQLK"
     gene            6451..7188
                     /locus_tag="PLB86_08850"
     CDS             6451..7188
                     /locus_tag="PLB86_08850"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HRC65281.1"
                     /translation="MADAVADAAAYLSGMPATPVPLERTDRAPWVWVSAQALRSRVDA
                     QALADAVREHPAVEAVAARADGWLEITLAPEWVDRTLADLAAPAGMPPAHLPAAPPAV
                     LGRRTLDNPGFVVLLAHSRACRVAGQALAGQERTGQATDQDDAAAFDPRALRDLSDPL
                     DVVLLTEILDAPRRLVATTPHQCAAALLAVAAAYLPWEERCPAIATRPGEPSTPQHWA
                     RARISAAGLGVLERGMALLGITAPERI"
     gene            7192..>7948
                     /locus_tag="PLB86_08855"
     CDS             7192..>7948
                     /locus_tag="PLB86_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRC65282.1"
                     /translation="MRAHEAGALHADGYRGPTWLQKPADVNALHPSLWATSVGRSRDG
                     VLSVGGVSVTDLATQFGTPAYIVDEADFRARAAEFVQTYNEIFEDLCGGADVYYAGKA
                     FLCTEIARWIRDDGLFLDVCSGGELAVAIRAGMPGSRIGMHGNNKSLAELEAAVDYGV
                     GRIIVDSFEEIDRLAAVAARLGRTASVLVRVTVGVEAHTHEFIATAHEDQKFGFSLAS
                     GAAAEAVRRILARAPHLRLLGLHSHIGSQIFDTS"
BASE COUNT         1097 a         2721 c         2790 g         1240 t
ORIGIN      
        1 cccttgggcg acgctgcgtc cgttggcgat gatgacgacg tcatcgacgg tctgctcgac
       61 ctcggagagc atgtggctcg agacgaggat cgtcttgccc tgcgccgcga ggtggcgcag
      121 gaactggcgc agccaacgga tcccttcggg gtccaggcca ttggccggtt cgtcgagcag
      181 cagcacctgc gggtcaccca acagggcggc tgcgagggcg aggcgttggc gcatgcccat
      241 cgagtagcca ccggcgcgct gccgagcggc ggccgggatg ccgacgagtt cgagcaactc
      301 gtcgacccgc tgggtcggga tgccgccggc cgcggcgagc acccgcaggt ggtcgcggcc
      361 gctccgacca gggtggaagc tgctcgattc gagcgctgcc ccgacggtcc gcagcgggcg
      421 ggcgaggtcg tggtaggtcg acccgccgat ggtggcggtg ccagaggtgg gccgcacgag
      481 cccgagcagc atccgcagag tggtcgtctt gccggctccg ttgggcccga ggaagccggt
      541 gacgcggccg ggctcgacgc tgaagtcgag gtggtcgacg gccaccaggc tgccgaagtt
      601 cttggtgagt ccgcgcactt ccagaggaag gccggggctg gatggttgtc cgctgggggg
      661 agagagggtc gtggtcgtca tcgtcggtcg atcgtcccct gagtagtagt cataccctcc
      721 atccaaacct gcccggcgac ctcggtgcca gtcgaacacg ctgttttcag ccgccgatca
      781 gggtcgggcc agggtcaacc ccggctctcg aggatcgacc gggagcggta gtcgaggtag
      841 gcgacggccg ccccgaactc ggcgaaggct gggttcgtcg tctgcccgtg gacgaatcgg
      901 tagtagatct gttgggcgat gacagccagc cggaacaggc cgaagacttc gtagaaacgc
      961 cactgctgag gcgacacgac gagcccggcg cgctcggtgt agcgctcgac gacctcggcc
     1021 cgggtgagca ttcccggggc gtgcgtgggc tgacgccgca tccgctgcat cacgaggtcg
     1081 tcgtcggcct gcacccagta ggccagcgcc gaccccaggt ccatcagcgg gtcgccgacg
     1141 gtggccaact cccagtccag gacaccgacg atccgggtcg gatcagtcgg gtcgaggatg
     1201 aggttgtcga agcggaaatc gttgtggatc aacaccgttg ccacgtcggg gggttgctcg
     1261 gcagccaacc acgccatgac cgcttcggca tccgggacgt cgggcgtgcg cgccttctgg
     1321 tagcgctcgg accagccggc gacctggcgc ccgacatagc cagggccgcg gccgagtccc
     1381 gccagccggg cggcgtcgac atcgacggca tgcaggtcgg ccagggtgtc gaccgcggcg
     1441 acgcacaacc gccgggtggt gtcggcgtcg agccccaact ccgcaggcag gttgcgccgc
     1501 aagatgattc ccgccacccg ctccatgacg tagaagtcgc tgccgagcac ctcgtggtcc
     1561 tgacagcagg cgaccatgcg cgggacgagc gggaagacgg gggccagcgc agcttgcagg
     1621 tcgtgctcac ggcgcatgtc gtgcgcaccc ttggccttgg tgcccgccgg cggtcggcgc
     1681 aggacgagat cgcggcctgc cggggcggtg ggggaggcgg cataccgaag gaggtaggtg
     1741 aggttagacg ccccgccgcc gaactgcccg acttccggta tgccgtccaa cccccttgcg
     1801 tccgtggcgt tctcgcgcag ccaggtagcg acgcgcggca cgtcnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnccgagg tggacctcgt ccggcccgtc ggcgaggcgc
     1981 agcgcccgag ccgtcgcgta tgccgccgcc agcggggtgt cggcgctgac gccggcgccg
     2041 ccgtgcaact ggatggccag atcgatgacc tggcatgcca tccgggggac ggcggccttg
     2101 atctcgctca cctcggacgc ggcgccctcg accccgaagg cgtcgagttt ccaggcgcag
     2161 tgcaggacgt gcagtcgggc ctggttgatg gcgatgcgcg cttccgccag gcgctcgcgg
     2221 ttgccaccga ggttggccaa gggcttgccg aaggccacgc gggtggacgc tcgttcgcag
     2281 gccgattcga gggagcgctc ggccaggccg atgagccgca tgcagtggtg cacccggccg
     2341 ggaccgagcc ggccctgggc gatgacgaag gcccgaccgg ggccgccgat gatcgccgag
     2401 gcgggcagtc ggacgtcggt gagcgagacc tcaccgtggc cgaggggttc gtcccattgc
     2461 cccagggccg gcatcacgcg ctcgatccgc acgcctgggg cgtcgagcgg gacgaggacc
     2521 atcgagtgtc gagcgtgctt gggggcctgc ggatcggtga cgcccatgaa aatgccgact
     2581 gcgcaatcgg ggtgccccac gccggtcgac caccacttgc gcccgtcgag gacgacctcg
     2641 tccccctcga tccgagcggt ggcggccatc gtcgtcgcgt ccgacgacgc gacgtccggc
     2701 tcggtcatga gaaacgcgct gcggatcctc ccgtccagca gtggctccaa ccaggtgtcc
     2761 ttctgctcct gcgagccgta cttcagcagg acctccatgt tgccggtgtc cggggcgttg
     2821 cagttgaaga ccaaaggggc caaggaactg cgcccggtct gctcagcgat gggggcgtag
     2881 tcgacgttgg acaaccccac gccgccatcg gtcccgaacc gcgcggcata cggaccctcg
     2941 tggccggctg ggaggaagag gttccacagc ccctgggtgc gggcgagggc ctgcaactcg
     3001 gggatgaggg ggtgaacggt ccacgggttc tcgcctcgat cacggcgggc ctggacgtcg
     3061 gcgaggatcg tggcctcgac ggggtcgatc cggctgtcga tgaagtcccg cacgcgggcg
     3121 gtgaggtcgg cagacctcgg tgacggggtg aagtccacag gccgacctta accccgcgtc
     3181 gatgcgcgct tccttccgct cccctgagcg cccgccgccg tagggctcgc ggttcattac
     3241 agtggagtga ctatgggcga atggctgttg gtgctcctcg cggtgatcct caccgtgggg
     3301 accgcgatct tcgtcgcgac cgagtttgcc ctggtcgccc tcgaccggcc gaccgttcaa
     3361 caggccatcg atgccggcga cacccgcgcc cgaggagtgc tcgcctccct gcgtcggctc
     3421 tcgacgcagt tgtccgcggc ccaggtcggg atcaccgtga cgacgctgct cgtcggttac
     3481 ctcgcccaac cctcgatcgg tcacctcctc gaggcgccgc tggccgccgt cctgccggcc
     3541 gggtccgtgg agtcggtcgc cgcgatgctc gccctggtgc tggtgaccct cttctcgatg
     3601 gtgttcggcg agttggtccc gcagttcctc ggcatctcgg cgcccttgcc gacggccaag
     3661 gtcgttgccg cgccggtccg gtggttttcg atgctggcca agccgctcat ctgggtgctc
     3721 aacggctcgg ccaacgggtt cctacgcggg atgggcatcg agccgcagga ggagttgtcc
     3781 ggcgctcgca ccccgcagga gttggccagc ctggtccgcc ggtccgcgct cgtcgggacg
     3841 atggagcgcg agacggccga acgcgtcact cgcaccttgg atttcggcga ccgcaccgcc
     3901 gccgatgtca tgacctcacg ggtgcgggcc gtcagcatcg atcgcaccgc cagcgccgcc
     3961 gatgtcgtgg ccttggcccg ccagaccggg cattcgaggt tccccgtcgt cggggacgac
     4021 tgggatgacg tcgatggcat cgtccacgtc aagcgggccc tcgccgtgcc gccggagcgt
     4081 cgcgtgttgg tcccggtgtc cgcgctcatg gtcgcgcacg tgacggttcc cgagaccatc
     4141 cgcctcgacg cgttgctcgt ccaactccgc caatcaggtc ttcaactggc cgtcgtcgtc
     4201 gacgaatacg gcggcacggc cggagtcgtc acccttgagg acgtcgtcga ggagatcgtc
     4261 ggcgacatct ccgacgagca cgactggtcg ggggtcagcg gtcgtcgggc caaggacggg
     4321 tcctggtcgg tgccgggcac ctggcgccct gacgaggtgc gcagccggct cggtgccccc
     4381 gtgccggacc acgcggcata cgcgacgctt gggggcttcc tcatggccgc gttggggcgg
     4441 gtccccgaac gtggtgacac ggtgtcgctg cccggctgga aggtgcgggt gcagcggatg
     4501 gaggggcggc gggtggctcg ggtccgcctc gtgccggtga cgccggcagt caccgacgat
     4561 ccggcgaacg ccacagggcg agaaggtgac gcgggcgcgc cgggcgcctc ccgagggccg
     4621 ggtgtccagc gagatccggg cagtcctcgg gcgccgcgcg gaccgctgca gccgagccag
     4681 cccgcgtcac cacctgggga ggtgacggga tgagtcactc gatggcgctc tggatctccc
     4741 tcgggctcct cctggccaat gcgttcttcg tcggcgcgga gttcgcggcg atggcggctc
     4801 gacgctcggc gctggagccg ttggccgctg ccggcagcgc ccgagccgcg acctgcctcg
     4861 ccgccctgga gcagatgggc tcgatgctcg ccaccgccca actcgggatc acggtgtgct
     4921 cggtcggcct gggcgcgctc gccgaggcgg ccctgcacga ggtgctgcac ccggtcttcg
     4981 cggctctgcc actgggggag gcctgggcca acggtctgtc cttcgccggt gcgctgttga
     5041 tcgtcgtcta tctgcacgtc gttgccggcg agatgatccc caaaaacctc gccctcgccg
     5101 ggccggagcg ggcggccctc gtgctcgtgc cggcgctcct catggtgtcg cgcgtgctac
     5161 gcccggtggt cgccgtcatg gaatgggtcg ccaaggggct ggtgcgggcg atcttccgga
     5221 tcgagcccaa ggacgagatc gcctcggcgt tcacggtcga ggaggtcacc cacatcctcg
     5281 ccgagagcga gcgcgagggg ctgatcgagg agcatcgcga ggggctcgtg cgctcggcgc
     5341 tggagttctc ggccaagctg gccgccgacg tcgccgtccc cgtcgatcgg ctggtcaccg
     5401 tcgccaaggg cgtcaccccg gaggacgtcg aacggctcgt cgcccgcacc ggcttctcgc
     5461 gctatccctt tctcgacaag gagggtcacg tcgcgggata cctgcacctc aaggacatcc
     5521 tgtatgccgc ggacgctgcc gcccgcactg agcgcgtccc ccgcaagcgg ctgcgccgtc
     5581 tggccaccgt cgcccccacg gaggagatcg aggacgtgct ggcgacgatg cgcggcaacg
     5641 gcacccacct ggcccgggtt gtcgacccca ccggtgaggt cattggggtg atcttcctcg
     5701 aagacatcgt cgaagaactc gtcggcacca tctcggacgc tgagacccgc tgacccgtcg
     5761 gccaagccgc cacacacccg gaaacgcgag aaccggccgt ccgcactctc gtgcggcgac
     5821 cggttctcac tacgtgcccc cggcaggatt cgaacctgcg cccccgcctc cggagggcgg
     5881 tgctctatcc cctgagctac gggggcctga accttcggtg tccatcatgt ggacagctca
     5941 tcagtggatg atgccgattg tcacgcctgg aggagggtat cagcctaggc tgaccaacgt
     6001 ggaacccacg ctgggggcag ggtggggggg gaatggtgtt gtgctcgttt gcgacgacac
     6061 cgaatccatc cgacggctgc tcagaatcaa ccttgagctt gatggcttcg aggttcttga
     6121 ggcatgcgac ggcgcctcgg ccatcgccct gctgcgcgcg ctccagctcg aagatcgcct
     6181 ccccgacgtc atcctgctgg acgcccacat ggctccgctg gacgggtggt gggcgctctc
     6241 ggagatccgt tcctgcccgc gactgtcgcg tatcccggtc gtcatggcca cggccgacac
     6301 ggtctgcgaa gacaaggcgg ccatgctcgc tcgtggcctg gacgcctgtg tccagaagcc
     6361 gttcgacccc gaggccgtcg tcgacctcgt cgccggtttc gtccgggagg gtcgggccca
     6421 cgtcccgcag ttgaagtgac actggcgcgc gtggccgacg cggtcgccga cgcggcggca
     6481 tacctgagcg gtatgccggc cacccccgtt cccctcgaac gcaccgaccg cgccccctgg
     6541 gtctgggtca gcgcccaggc ccttcgcagc cgcgtggacg cccaggcgtt ggccgatgcg
     6601 gtccgtgagc accccgccgt cgaggcggta gcggcccgcg ccgacggctg gctggagatc
     6661 accctcgccc ccgagtgggt cgaccgcacc cttgccgatc tcgcagcgcc cgccggtatg
     6721 ccgcccgctc acctcccagc cgcgccaccg gctgtccttg gccggcggac gttggacaac
     6781 cccggcttcg ttgtcctgct cgcccactcc cgggcctgcc gggtcgccgg tcaggcgctc
     6841 gccggtcagg agcgcaccgg tcaagcaacc gatcaggacg acgccgctgc cttcgacccg
     6901 cgagccctgc gtgatctgag cgatcccctc gacgtggtcc tgcttaccga gatcctcgat
     6961 gccccgcgtc ggctggtcgc cacgaccccg caccagtgtg cggcggcgct gctggcggtc
     7021 gcggcggcat acctgccctg ggaggagcgc tgccccgcga tcgcgacccg accgggggag
     7081 ccgagcaccc cccagcactg ggcccgagcg cgcatctcag cagccggatt gggcgtcttg
     7141 gagcgtggaa tggcgctgct cgggatcacc gcccccgaga ggatatgagg catgcgcgca
     7201 cacgaggccg gtgcccttca cgccgatggc taccggggac cgacctggct gcagaagccg
     7261 gcggacgtca acgccctgca ccccagcctg tgggccacga gcgtggggcg ttcccgcgac
     7321 ggtgtcctct cggtcggggg cgtgtcggtc accgacctgg cgacgcagtt cgggacgccg
     7381 gcctacatcg ttgacgaggc agacttccgg gcccgcgcgg cggagttcgt gcagacctac
     7441 aacgagatct tcgaggacct gtgcggcgga gccgacgtct actacgcggg caaggcgttc
     7501 ctgtgcaccg agatcgcccg ctggatccgt gacgacggcc tgttcctcga cgtgtgcagc
     7561 ggcggcgagt tggccgtcgc catccgggcg gggatgcccg ggtcgcgaat cggcatgcac
     7621 ggcaacaaca agagcctggc ggagctcgaa gcggccgtcg actacggcgt cgggcggatc
     7681 atcgtcgact ccttcgagga gattgatcgg ttggccgccg tcgccgctcg gcttggtcgc
     7741 acggcgtcgg tcctcgtccg ggtcacggtc ggcgtcgagg cgcacaccca cgagttcatc
     7801 gccaccgccc acgaggacca gaagttcggg ttctccctcg cctcgggtgc cgcggccgag
     7861 gcggtccggc ggatcctcgc ccgcgccccc catctgcggc tgctcggcct gcacagccac
     7921 atcggctcgc agatcttcga cacctccg
//
LOCUS       DAORPW010000102         7616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2423_length_7616_cov_867.412379, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000102 DAORPW010000000
VERSION     DAORPW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2423_length_7616_cov_867.412379"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLB86_08860"
     CDS             <1..477
                     /locus_tag="PLB86_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRC65283.1"
                     /translation="LRHVGLKALGSLRMEKAYRDYGHDIDNTDNLAMVGLGFAVAKDK
                     PAPWIGRGPTLAALAAGPPQSRLVQVLLADPEPLLFHAEILLRDGVAVGDVRSGSYGW
                     TLGGAVGLAMVTNPDGPATADWLSAGTWEVDIAGQRYAAHVSLRPLYDPTSARVNA"
     gene            663..1058
                     /locus_tag="PLB86_08865"
     CDS             663..1058
                     /locus_tag="PLB86_08865"
                     /inference="COORDINATES: protein motif:HMM:NF025379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HRC65284.1"
                     /translation="MPTITETLKTKLDEFELERRIDELTVATEKVVKRAIEQAGDLAH
                     DNRDKVTTLLDKAGSAIDQRTDGKYHDTVGKVRAQVVSGVDKLATKRPTHHTPFDTDN
                     EATEANEAPTSNETPQDAPTTWSAHTDPV"
     gene            1172..1660
                     /locus_tag="PLB86_08870"
     CDS             1172..1660
                     /locus_tag="PLB86_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC65285.1"
                     /translation="MTSPRDVVTVQRLIPAPASAIFALLADPSRHPEIDGSGTVLHPS
                     SPGSRHLGLGDTFGMSMKLGVPYSTRNVVIEFEEDRRIAWQTLAAAPIDKVLTGRVWR
                     YELEPVDGGTIVRETWDISREATLSRPIVRRLMGAQTRINMARTLERIESAVAGTDLR
                     SI"
     gene            1682..4801
                     /locus_tag="PLB86_08875"
     CDS             1682..4801
                     /locus_tag="PLB86_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018769559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRC65286.1"
                     /translation="MHDQRSIILARVRRVLAERIRPAETTVVTPLDAAAWEVTEPDGI
                     PGQGEPVPAQVALAADFTPTAVGMPWGPPWGTTWFRFRASVPPDLRGAPLEAVIDLGW
                     TSGSVGGQAEGLVFDKGGRIIKGIHPRNAWVPVRPNDKGKVRFYVEAAANPEVLRSRS
                     FAPTSVGRKETADPTPLYRLARADLVEVHHEVRELIADVVTLLGLAEVLPETATRAWT
                     VLRALDEAMDALDLADIVGTAAAARAVLAPALAVRAAEGTHEISAIGHAHIDSAWLWP
                     VRETRRKVARTVADVLHLAESRPGVVFALPAAQHGAWLEEDQPDLFARLQAAVAAGAV
                     VPVGGMWVEPDGNLLAGESVCRQLTFGARWFAERLGHRCDEVWLPDSFGYSAALPQLA
                     RLAGMRWMLTQKISWNEVDRFPHHSFLWEGIDGTRIFTHFPPADTYSSELTSVDLTRA
                     ASAFADKGRARRSLLPFGYGDGGGGPTREMFAQADRVADLDGSPRVRVESPAAFFERA
                     EAEHRDPSVWVGELYLERHRGTFTSQARTKAGNRRCEGLLREAELWWTSVALRGLSDY
                     PAQALDRLWRQVLLGQFHDILPGSSIGWVHDEVERTHAEVEAELEDLITRALALLCNE
                     GEAPQDADADGPTEWVSFSADPLAGDGVALGGTAYLGDSEPSAPAVTVVATGTAAVLD
                     NGRLQVTIDARGLVTSVRDLAADRELIPPGTVGNLLQCHPDLPNAWDAWDLDPFYRNT
                     VVDLTEVDELGVQTLDDGRAEVRVVRPLGTRGSQATQTLSLAPGAEHLDIEVEVDWRE
                     RDTILKLAWPLDVHTAEARFETQFGHVTRPIHENTTWDRYRFEVPALRWVHVGEPGYG
                     IAIANARTYGWDVTRSARAGGATFTTVRASLLRGSQFPDPDADLGTHRFGFRLVPGAD
                     VGGAVAAGYAVAHPPRVVAGRAGRHTEPVVRVEGAVVETVKPAEDGSGALVVRLYEHL
                     GRRAAATLALAPDVPLDLAHAHEVDLHEQPRDTIDQPSAVRGLDEFGSLRLALGPFQI
                     LTVRISTKS"
     gene            4849..6117
                     /locus_tag="PLB86_08880"
     CDS             4849..6117
                     /locus_tag="PLB86_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC65287.1"
                     /translation="MSRAPDEAVRLRTPWQSRPFRVLTIGMVISTFGSSITPVAIAFA
                     VLSLGGTATQLGLVVAAYALAEVATVLVGGVLGDRLPRAAVVQGAAMASAVTQGVLGL
                     SLVKGWSSIGLVALLSALTGIVGSLSIPSASAMTQQMVPGYLLQRAISIRRLLGNSAS
                     VIGFGAGGMLVAAVGPGWAILVDAATFLVASILLASVSLPSPAAEPGRSVLADALVGA
                     REVMRHTWLWVLIAVALVYHLFYASAQGVLGPIVVQMGYGEAAWGWALAVLMIGFMAG
                     GLVTLRWMPRHALYIGTAFLTLTACFPAALAANLNLGLVLTGAFLHGFGLELFSVAWD
                     LSIQENVAPDKLARVYGFDVLGSFIARPVGLALTGPLAEAVGYADWLWVVTGVVLAAT
                     LLALTVPAVRTLERQLPARTNDPDVQPTGG"
     gene            6127..6861
                     /locus_tag="PLB86_08885"
     CDS             6127..6861
                     /locus_tag="PLB86_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRC65288.1"
                     /translation="MLTDQPAHRVVATVSDTPVGDIDVLTWSPADAHIAEPLPMLFAH
                     DGPELDVFAELTALIGSCVASGRLPRMRVTLLAPGDRNPRYAASAAYAAALADHVVPL
                     QLRAHPSEHPPVLVGPSLGGLAALHAEWTHPGTFGGLLMLSGSFFTPELDPQEKGFEF
                     WEQVVGFVDRVHTSPAPSRPEVALGWGTQEENQFNNALMAQRLGELGLPVQTATVDNG
                     HNFTCWRDLLDLLLPDLLTRTWMPRT"
     gene            6865..7611
                     /locus_tag="PLB86_08890"
     CDS             6865..7611
                     /locus_tag="PLB86_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRC65289.1"
                     /translation="MDTARVELSAAGFDRPGTVLRYGHWGRPVLVFPCEAGRAWDFAD
                     RGMLAAVTPLVDAGRVKLYCVDSFDEATWSNRGLSIEDRARGHLAYEGWITGPVLDWV
                     RHDSPGAGDPVTTGVSMGAFHAVQFALRRADLFPQALAMSGSYDPSWWHAWGEPGDAA
                     YATNPSWYVPDLEGGHLDWLRSRLDLVLVVGRGPFETEPTRALPSTRLLAGMLQDKGI
                     RAELDEWGEDVAHDWPWWQQQLAHHLPRFL"
BASE COUNT         1060 a         2825 c         2567 g         1164 t
ORIGIN      
        1 ctgcgccacg tcggcctcaa ggcgctcggc agcctgcgga tggagaaggc ctaccgcgac
       61 tacggccacg acatcgacaa caccgacaac ctcgcgatgg tcggactcgg cttcgccgtc
      121 gccaaggaca agccggcccc gtggatcggc cgcggaccga cgctcgccgc cctggccgcc
      181 ggccccccgc agagccgact cgtccaggtg cttctcgccg accccgagcc gctgctcttc
      241 cacgccgaga tcctgctgcg cgacggagtg gcggtcggcg acgtccgctc cggctcctac
      301 ggctggacgc tcggcggcgc cgtcggcctg gcgatggtca ccaaccccga cggcccggcg
      361 accgcggact ggctctcggc cggcacctgg gaggtcgaca tcgcgggcca gaggtatgcc
      421 gcccacgtct cgttgcgccc gctctatgac ccgacgagcg cccgggtcaa cgcctgaccg
      481 agcggcgacg cctgtcccag gcaacgcctc ggcgccgctt ccggccgggt tccgatggcc
      541 agggggaggc cagggtgaac cctgagcgct ccgagggaac accgccccag cagggcacgt
      601 tgtgctggac ggctccggaa gttcccggcg ccgcacacca cgcaccgcag gggagacccc
      661 caatgcccac catcaccgag accctcaaga ccaagctcga cgagttcgag cttgagcgcc
      721 gcatcgacga gctcaccgtc gccaccgaga aggtcgtcaa gcgcgccatc gagcaggccg
      781 gcgacctcgc acacgacaac cgcgacaagg tcaccacgct cctggacaag gccggctcgg
      841 cgatcgacca gcgcaccgac ggcaagtacc acgacaccgt cggcaaggtc cgcgcccagg
      901 tggtgtccgg cgtcgacaag ctcgccacca agcgcccgac ccaccacacc ccgttcgaca
      961 ccgacaacga ggcgaccgag gccaacgagg cgcccacgtc caacgagacc ccgcaggacg
     1021 ctccgaccac ctggtcggcg cacaccgacc cggtctgatc ccctgccgcc cgtcgcggcg
     1081 ctgatttagg tgtggtcgaa cgtgggccct ggcccccgtt cgaccacact catgttccgg
     1141 ccccgtccgg gccgcgatag cgtcatcgac catgaccagc ccccgcgatg tcgtcaccgt
     1201 ccaacgcctc atcccggcac cggcgtcggc gatcttcgcc ctgctggccg acccctcccg
     1261 ccaccccgag atcgacggat ccggcaccgt gctgcaccca tcgagccccg gctcccgaca
     1321 cctgggcctg ggtgacacct tcggcatgtc gatgaagctg ggcgtcccct actccacccg
     1381 caacgtcgtc atcgagttcg aggaggaccg ccggatcgcc tggcagaccc tcgcggccgc
     1441 ccccatcgac aaggtgctca ccggccgggt ctggcgctac gagctcgagc ccgtcgacgg
     1501 cggcaccatc gtgcgcgaga cgtgggacat ctcccgcgag gcgaccctgt cgcgaccgat
     1561 cgtccggcga ctcatgggcg cccagacccg gatcaacatg gcccgcacgt tggagcggat
     1621 cgaaagcgcg gtcgccggga ccgacctacg ctctatctga cacctagtca gaggagtccc
     1681 ggtgcacgat cagcgctcca tcatcctggc ccgggtgcgc cgagtcctcg ccgagcggat
     1741 ccgcccggcc gagaccacgg tggtcacccc gctcgatgcc gcagcctggg aggtgaccga
     1801 gccggacggc ataccggggc aaggggagcc cgtccccgcg caggtggctc tcgccgccga
     1861 cttcaccccc accgcagtgg gtatgccgtg gggtcccccc tgggggacga cctggttccg
     1921 cttccgcgcg agcgtgccgc cggacctgcg cggcgccccc cttgaagcag tcatcgacct
     1981 cggctggacg agcgggtcgg tcggtgggca ggccgagggc ctggtgttcg acaagggcgg
     2041 ccggatcatc aagggcatcc acccgcgaaa cgcgtgggtt ccggtgcggc ccaacgacaa
     2101 gggcaaggtg cgcttctacg tcgaggccgc cgccaacccc gaggtgctgc ggtcccgctc
     2161 cttcgcgccc acgagcgtcg ggcgcaagga gaccgccgac ccgaccccgc tctatcgcct
     2221 ggcccgagcc gacctggtcg aggtgcacca cgaggtccgc gagctcatcg ctgacgtggt
     2281 gacgctgctc ggcctggccg aggtgctgcc cgagaccgct acccgcgcct ggaccgtcct
     2341 gcgcgccctc gacgaggcga tggatgccct cgacctggcc gacatcgtcg gcaccgccgc
     2401 ggccgcccgc gccgtcttgg cgccggcgct cgccgtgcgg gccgccgagg gcacccacga
     2461 gatcagcgcc atcgggcacg cccacatcga ctcggcctgg ctctggccgg tccgtgagac
     2521 ccgacgcaag gtcgcccgga ccgtcgccga cgttctccac ctggccgagt cccgtcccgg
     2581 cgtcgtcttc gcgctgcccg ccgcccagca cggagcgtgg ctggaggagg accagcccga
     2641 tctcttcgcg cggctgcagg cggccgtcgc cgccggtgcc gtcgtcccgg tcggggggat
     2701 gtgggtcgag ccggacggca acctgctcgc cggcgagtcg gtatgccgcc agctcacctt
     2761 cggcgcgcga tggttcgccg aacgcctggg tcaccgttgc gacgaggtgt ggttgcccga
     2821 ctcgttcggc tattcggcgg ccctgcccca gctcgctcgg ctggccggaa tgcgctggat
     2881 gctcactcag aagatctcgt ggaacgaggt cgaccgcttc ccgcaccact cgttcctctg
     2941 ggagggcatt gacgggacgc gcatcttcac gcatttccct cccgccgata cctattcgtc
     3001 cgagttaacc tcggttgatc tcactcgggc ggccagcgct ttcgccgaca agggccgggc
     3061 acgccgatcc ctgctgccgt ttggttacgg cgatggtggc ggcggcccga cccgagagat
     3121 gttcgcccag gccgaccgcg tcgccgacct ggacggctcg ccccgggtgc gggtcgagag
     3181 cccggccgcg ttcttcgagc gggccgaggc cgaacaccgg gatccgtcgg tgtgggtggg
     3241 cgagctctat ctcgaacgcc accgtggcac cttcaccagc caggcccgca ccaaggccgg
     3301 caatcggcgc tgcgaagggc tgctgcgcga ggccgagctc tggtggacga gcgtcgcgtt
     3361 gcgaggtctc agcgactatc cggcgcaggc cctggatcgg ctctggcgtc aggtgctgct
     3421 cggtcagttc cacgacatcc tgcctggctc gtcgatcggc tgggtccacg acgaggtcga
     3481 gcggacccat gccgaggtcg aagccgagct cgaggacctc atcacccggg cgctcgccct
     3541 cctctgcaac gaaggcgagg caccccagga cgccgatgcc gacggcccga ccgagtgggt
     3601 gtcgttcagc gccgacccgc tggccggcga cggggtcgcg ttggggggca cggcatacct
     3661 gggcgacagc gagccgagtg ccccggcggt gacggtcgtc gccaccggga ccgcggcggt
     3721 cctggacaac ggccggttgc aggtcaccat cgacgctcgc gggctggtca cctcggtgcg
     3781 cgatctcgcg gccgaccgcg agctcatccc gcccgggacc gtcggcaacc tcttgcaatg
     3841 ccaccccgac ctgcccaatg cctgggatgc ctgggacctc gacccgttct accgcaacac
     3901 cgtggtggac ctcaccgagg tcgacgagct cggcgtccag accctcgacg acggcagagc
     3961 cgaggtccgc gtggtccgtc ccctgggcac gcgcggctcg caggcgaccc agaccctctc
     4021 cctcgcccct ggcgccgagc acctggacat cgaggtcgag gtcgattggc gcgagcgcga
     4081 cacgatcctc aaactcgcct ggccgctgga cgtccacacc gccgaggcgc gcttcgagac
     4141 ccagttcggg cacgtcacgc gccccatcca cgagaacacc acgtgggacc gctaccgctt
     4201 cgaggtgccc gcgctgcggt gggtgcacgt cggcgagccc ggctacggca tcgccatcgc
     4261 caacgcccgc acctacgggt gggacgtcac ccgcagcgcc cgcgccggtg gcgcgacgtt
     4321 cacgacggtg cgggcgagcc tgctgcgcgg ctcgcagttc cccgacccgg acgccgacct
     4381 cggcacccac cgcttcggtt tccgcctcgt ccccggcgcc gacgtcggag gtgccgtcgc
     4441 cgcggggtat gccgtggccc acccgccgcg cgtcgtcgcg ggccgggccg ggcggcatac
     4501 cgagcccgtg gttcgcgtcg agggagcggt ggtcgagacg gtcaaacccg ccgaggacgg
     4561 cagtggcgcg ctggtcgtgc ggctctacga gcacctcggc cgccgtgccg cagcgacctt
     4621 ggccctcgcg ccggacgtgc ccctggacct cgcccacgcg cacgaggtcg acctgcacga
     4681 gcaaccccgg gacacgatcg accagccatc ggccgttcga ggcctcgacg agttcggcag
     4741 cctgcgcttg gcgctggggc ctttccagat cctcaccgtg agaatttcga cgaaatcctg
     4801 aacattctgt gacattccat cgtccgctga ccctttacgg tggaacctat gtcgcgcgcc
     4861 cctgacgaag cggtgaggtt gcgcacgcca tggcagtcac gaccgttccg ggtgctgacg
     4921 atcggcatgg tgatcagcac cttcggaagc tcgatcaccc cggtcgccat cgcgttcgcg
     4981 gtgctctccc tcggcgggac ggcaacgcaa ctgggtctgg tcgtcgcggc gtatgccctg
     5041 gctgaggtcg ccaccgtcct tgtcggtggc gtgctcggtg accggctgcc gagggcggcc
     5101 gtcgtgcaag gtgccgcgat ggcgtcggcg gtgacccaag gagtgctcgg gctgagcctg
     5161 gtcaagggct ggtcgagcat cggcctcgtt gcgctcctca gtgccctcac cgggatcgtc
     5221 gggtcgctgt cgatccccag cgcctcggcg atgacccagc agatggtccc cggatacctg
     5281 ctgcagcgag cgatctcgat ccggcgactg ctcggcaact ccgcgagcgt catcgggttc
     5341 ggggccggag gcatgctcgt cgccgccgtc ggcccggggt gggcgatcct cgtcgacgcg
     5401 gcaaccttcc tcgtggccag catcctgctc gcctcggtgt cgctgccctc gcccgccgcc
     5461 gagcccggtc gctcggtgct ggccgatgcc ctcgtcggcg cccgggaggt gatgcggcat
     5521 acctggttgt gggtgctcat cgcggttgcc ctcgtttatc acctcttcta cgccagcgcc
     5581 caaggcgtcc tgggcccgat cgtcgtccag atgggctacg gcgaagcggc ctggggctgg
     5641 gctctggcgg tgctgatgat cggcttcatg gccggcgggt tggtcacctt gcggtggatg
     5701 ccccggcatg cgctctacat cgggaccgcg ttcctcacgc tcaccgcctg cttcccggct
     5761 gcgttggctg ccaacctcaa cctcgggctg gtcctcaccg gggccttcct gcacggtttc
     5821 ggcctggagt tgttcagcgt tgcctgggat ctgtcgatcc aggagaacgt cgcccccgac
     5881 aaactggcgc gggtctacgg cttcgacgtc ctcgggtcgt tcatcgcccg gcccgtcggc
     5941 ctggccctca ccgggccgct cgcagaggcg gtggggtacg ccgactggtt gtgggttgtc
     6001 acaggggtcg tcctcgcggc gaccctgctg gccctcactg tgcccgcggt gcgaaccctc
     6061 gagcgtcaac ttccagcccg gaccaacgac ccggacgtcc agcccaccgg cggataacgt
     6121 ctggggatgc tcaccgacca accggcgcac cgagtcgttg ccacggtgtc cgacactccg
     6181 gtcggcgaca tcgacgtgct cacctggtca ccggccgacg cgcacatcgc cgaaccgctg
     6241 ccgatgctct tcgcccacga cggcccggag ttggacgtgt tcgccgagct caccgccctc
     6301 atcggctcct gcgtcgcctc cggacggctg ccacggatgc gggtcacctt gctcgccccg
     6361 ggagaccgca atccccggta tgccgcgtcc gcggcatacg ccgctgccct cgccgaccac
     6421 gtcgtgccgc tgcagttgcg cgcccacccg agcgagcacc cgccggtgct cgtcgggccg
     6481 agcctcggcg ggcttgcggc gctgcatgcc gaatggaccc acccggggac cttcgggggc
     6541 ctgctcatgc tgtcggggtc gttcttcacg ccggagttgg acccccagga gaaggggttc
     6601 gagttctggg agcaggtcgt cgggttcgtc gatcgcgtgc acacctcgcc ggctccgtcg
     6661 cggccggagg tggccttggg gtggggcacc caggaggaga accagttcaa caacgccctc
     6721 atggcccagc ggctgggaga gttgggcctg cccgtgcaga cggcgaccgt cgacaacggg
     6781 cacaacttca cctgttggcg tgacctgctc gacctgttgc tgcccgatct gttgacccga
     6841 acctggatgc cgaggacctg acgaatggac accgctcgcg tggagctttc cgcagccggc
     6901 ttcgaccggc ctgggacggt gctgcgttac gggcactggg ggcggccggt gctggtgttc
     6961 ccctgtgagg ccggtcgggc gtgggacttc gccgaccgag ggatgctcgc cgcagtcacg
     7021 cccctcgtcg acgccggtcg ggtcaagctc tactgcgtcg actccttcga cgaagcgacc
     7081 tggtccaacc gcgggttgtc gatcgaggac cgggcccgtg gacacctggc ctacgaggga
     7141 tggatcaccg ggccggtgct ggattgggtg cgccacgact cgccgggggc cggcgacccg
     7201 gtgacgaccg gcgtgagcat gggcgccttc cacgccgtcc agttcgccct gcgccgcgcc
     7261 gacctcttcc cgcaggccct ggcgatgtcg ggaagctacg acccctcctg gtggcacgcg
     7321 tggggcgaac ccggcgacgc ggcatacgcg acgaatccgt cctggtatgt cccggatctg
     7381 gagggtgggc acctcgactg gttgcgctcg cgactggacc tcgtgctcgt cgtcggtcgc
     7441 gggccgttcg agaccgagcc gacccgggcg ctgccgtcga cccggctgct ggcggggatg
     7501 ttgcaggaca aggggattcg ggcggagctc gacgagtggg gcgaggacgt cgcccacgac
     7561 tggccgtggt ggcagcagca actggcccac cacctcccgc ggttcctctg acgcct
//
LOCUS       DAORPW010000103         7577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2447_length_7577_cov_885.840601, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000103 DAORPW010000000
VERSION     DAORPW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2447_length_7577_cov_885.840601"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLB86_08895"
     CDS             complement(<1..395)
                     /locus_tag="PLB86_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC65290.1"
                     /translation="MAQRNHRVPVSYGASIMALEVLPARPVAEGVPTVVLAHGWCLDH
                     TSWHKVVADLQARRNVRVVLFDQRGHGKSSMGEVGDPTVRILGDDLREVIDAVAPEGD
                     LVLAGHSMGGMSVMAYAGMHYEHFTERVKG"
     gene            complement(434..1270)
                     /locus_tag="PLB86_08900"
     CDS             complement(434..1270)
                     /locus_tag="PLB86_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC65291.1"
                     /translation="MKSVQGKTVLITGAAMGMGRLFAERAIAEKAKAVVLWDLNEVAL
                     QRTLEELTGRGSEVTAYLVDVGDLAAVEASAAAVLAEVGAVDVLVNNAGVVRGNQYFW
                     ESDSGRDTRLTVAVNTLAPMYVARAFLPGMIAGSGEARLLNLASAAGFTANPRMAVYA
                     ASKWAVIGWSDSVRLELKQAGVEHVKVTTVCPYYVNTGMFDGAKSAPLLPILEPAHVV
                     DEAWRAMCAGGSFVVLPRTVMLSEVLKGLVPISVRDFISDNVIGVYHTMEDFTGRAEQ
                     QA"
     gene            complement(1343..3403)
                     /locus_tag="PLB86_08905"
     CDS             complement(1343..3403)
                     /locus_tag="PLB86_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HRC65292.1"
                     /translation="MPRKRLVVVNITLQGPEWDFDEKVTFAGRDFRLIRVGTTGDLDA
                     AEKLVWQWSLKADAVAVTGVREAQAAGVFKGDPLEVRQVLETTARVPVRDGSMLADVL
                     QEWAIRRVQAEMPGYFNNARTVVLSGKNYERTIRVLREFTANIDFVDPALRTDVPIAL
                     NTNPVVAKAAEVGMWTWRLTPGFFKDHSQGPVEWVSDKLAHRAARDADIIVGSYAELM
                     HFGLEDLAGKTVVTNAVSDGRLAELTSRGVDLIIDATPRPFQFMVVTAMLEAMAAAIN
                     TEERELTTDDLLELIQAAELEPRLLRPNGDKRKSRFAFVIHPLSAQYFNNVEAIGALS
                     RVPGMGDVVEKAMAYIPPFIYSHVTGIESPTGAEAEGWLISVGGTPKEMLAHSPEFTY
                     SRLLEAADLARKLGAQVMGLGAFTKVVGDAGVTVAKRAPLPVTTGNSYSASGALWAAH
                     AALRKLGIARVDDHGIIHGKAMVVGATGAIGSVCARLLALVTDELWLVSPETAKLLAI
                     KKDIESENPRAVLHVTARPGDALAEMDLIVTATSAAGNKVLDIMKVKPGCVITDVARP
                     LDMSAEDVAKRPDVLVIESGEIELPGDVHMGNIGLPAGVAYACLAETVVLALEGRYET
                     FTVGRNIEWPKVKEIYQLGLKHGMKLATISGVGGVFTDDDFARVREAALAARATASSD
                     ANAT"
     assembly_gap    3495..3594
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3595..4707)
                     /locus_tag="PLB86_08910"
     CDS             complement(<3595..4707)
                     /locus_tag="PLB86_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRC65293.1"
                     /translation="MGTRIAASDLTWLLMDRPNNLMHVHGLLTFDELPEWEALRDAIF
                     ERVVTKYRLLSQVAVQHDGHWEWEDDVDFSVDRHVLRVVLADSQPETLRAYVSSQFSV
                     PFDRSRPLWDMQLISGPPEDEGGGAMLTRFHHGLGDGIRLVQLLIGTCDPTDGATPHT
                     VGRGSDGGSHGPLDAVRHLAEHSVKDTIDAVTHSGSLVAQAGRTVIATMNPLALPHHL
                     ESALDLVRHPTKLIDVLTGFTSDDNETSNSWREVGRLLLHEKADAGAWSGHPDVDKGV
                     AWTEGISLSSVKAVAQAFNGTVNDALMAAVSLALTDYLGERGVTDITDVAWMMPVSLQ
                     PVDGSLPSTLGNHFAVVLFEMPLGIADPAELIEQIHH"
     gene            4892..6514
                     /locus_tag="PLB86_08915"
     CDS             4892..6514
                     /locus_tag="PLB86_08915"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HRC65294.1"
                     /translation="MTPASSGNRSSAHRPGPLDPEHVLAATYALDQSYVRGLTDQLVA
                     TSGTSSRLLAPATGQPLGNIPTSTPADVLSAMARAKEAAKDWGLSSFAERSEVLMRLH
                     ELILDRREEIMDLICWESGKSRIHAFDEPLHVALTARYYARTLDRHLAPKRRDGVVPF
                     LTQIEEHRTPKGVVAIISPWNYPFTMALVDGIAAIAAGNAVVSKPDHHTPLSALLGLA
                     LLEEAGLPEGVWQMVTGAGRVLGTPMIENADYVCFTGSTATGRVIARQCADRLIGCSL
                     ELGGKNPILVLRDADLDRAAEGAVRAAFSNGGQLCVSTERLYVDAEVHDEFVEKFVAR
                     TKAMRLGAGHEWGIDMGSLISAEQLTTVADHVDDAVHHGARVLAGGRARPDLGPYFYE
                     PTILDGVTPDMACHADETFGPVISVYPFTTEDEAVDLANAGDYGLNAAVFSRDNKRAR
                     LIANRIKCGTVNINEAFSATFASLDAPMGGMRSSGIGRRQGAEGIHRYTEVQSVATQY
                     LLRFGPHWGLTDEAYAAVMTGAVKLLTRLGRA"
     gene            6514..>7577
                     /locus_tag="PLB86_08920"
     CDS             6514..>7577
                     /locus_tag="PLB86_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRC65295.1"
                     /translation="MTTAFDYDVVIVGSGFGGSVAALRLTEKGYSVAVLEAGPRFEDA
                     DLPDTSFDVRNYLFAPSLGCYGIQRIDLLNDCLILSGAGVGGGSLVYANTLYQPPAAF
                     YDDPQWRDITDWKTELAPYYDQATRMLGVITNPWHTPSDEVIRQVAEEMGKGDTFRYT
                     PVGVLFPRDGESKGQPVDDPYFGGEGPRRHTCTGCGECMTGCRHNAKNTLVKNYLYLA
                     EKHGAHVHPLTTVTRVVPRHGGGYDVHTTNTRAKTKRRAARQSRTYAAQQVIFAASAL
                     GTQRLLHTMKFTGELPRLSDTLGRLTRTNSESILGAGAPKDDPRDFTEGVAITSSFHP
                     DEHTHIEPVRYGRGSNVMSLL"
BASE COUNT         1222 a         2606 c         2450 g         1199 t
ORIGIN      
        1 cccttgaccc gctcggtgaa gtgctcgtag tgcatcccgg cgtaggccat gaccgacatg
       61 ccacccatcg agtgcccggc caggacgaga tcaccctcgg gcgccaccgc gtcgatgacc
      121 tcgcgcaggt cgtcaccgag gatgcggacc gtggggtcgc cgacttcgcc catgctcgac
      181 ttgccgtgcc cccgctggtc gaagagcacc acccggacgt tccgccgggc ctgaaggtcg
      241 gcgacaacct tgtgccaact cgtgtggtcc aggcaccacc cgtgggccag cacgacggtc
      301 ggcacaccct ccgcgaccgg gcgcgcaggg aggacctcca gggccatgat gctggcaccg
      361 taggagaccg ggacgcggtg attgcgctgc gccaaggctc agtccttccg acctgctggg
      421 tccgacgacc tggtcacgcc tgctgctcgg cgcggccggt gaagtcctcc atcgtgtggt
      481 agaccccgat gacgttgtcc gagatgaagt cgcgaacgct gatcggcacc aagcccttga
      541 gcacctcgct gagcatgacg gtgcgcggca ggacgacgaa cgatccgccg gcacacatcg
      601 cccgccaggc ctcgtcgacg acatgcgccg gctcgaggat cggcaggagc ggcgcggact
      661 tggcgccgtc gaacatgccg gtgttgacgt aataggggca cacggtcgtc accttgacgt
      721 gctcgacccc ggcctgcttg agctccagcc gcaccgagtc ggaccagccg atgaccgccc
      781 acttggacgc ggcatacacg gccatccgcg ggttggccgt gaaaccggcg gccgaggcga
      841 ggttgagcag gcgcgcctcc ccgctgccgg cgatcatccc cggaaggaag gcccgcgcga
      901 catacatggg ggcgagggtg ttgaccgcca ctgtcaggcg agtgtcccgc cccgagtcgg
      961 actcccagaa gtactggttg ccgcggacga caccggcgtt gttgaccagg acgtccactg
     1021 ccccgacctc ggccaggacg gcagcggcgc tggcctccac cgcggcgaga tcgccgacgt
     1081 cgacgaggta tgccgtgacc tcacttccgc gtcccgtcaa ctcttcgagg gtgcgctgca
     1141 gagcgacctc gttgagatcc cagaggacca ccgccttggc cttttcggcg atggctcgct
     1201 cggcgaacaa gcgccccatg cccatcgcgg caccggtgat caggaccgtc tttccttgga
     1261 ctgacttcac gacactcctc catcgggtct ctccccatcc tgacaggaag agcacctggt
     1321 gacatggccc aggcgacgtg gcctacgtcg cgttggcgtc gcttgacgcg gtggcgcgtg
     1381 cggccagggc ggcctcgcga actcgggcga aatcgtcgtc ggtgaatacg ccgccgacgc
     1441 cggagatcgt cgccaacttc atgccgtgct tgagcccgag ttggtagatc tccttgacct
     1501 tgggccactc gatgttccgt ccgaccgtga acgtctcgta gcgcccttcg agcgcgagga
     1561 cgacggtctc ggcgaggcag gcataggcga cgccagcagg caggccgatg ttgcccatgt
     1621 ggacgtcacc gggcaactcg atctcgccgg actcgatgac gagcacgtcg ggacgtttgg
     1681 cgacatcctc ggcagacatg tccaacggcc gggccacgtc ggtgatgacg caccctggct
     1741 tgaccttcat gatgtcgagc accttgttcc ccgcggcgga ggtggcggtg acgatgaggt
     1801 ccatctcggc gagtgcgtcg cccggtcgag cggtgacgtg caagacggcc cgggggttct
     1861 cgctctcgat gtccttcttg atggccagca acttggcggt ctcgggcgag accaaccaca
     1921 actcgtcggt gacgagcgcc aacagccggg cacagaccga gccgatcgcc ccagtggccc
     1981 cgacgaccat ggccttgccg tggatgatgc cgtggtcgtc gacgcgggcg atgcccagtt
     2041 tgcgcagggc cgcgtgggct gcccagagtg ccccggaggc gctgtagctg ttccccgtcg
     2101 tcaccggcag cggtgcccgc ttggccacgg tgacgccggc gtcgccgacg accttggtga
     2161 aggcaccgag ccccatcacc tgggcgccca acttgcgggc caggtcggcg gcctccagca
     2221 gacgtgagta ggtgaactcc gggctgtggg caagcatctc cttgggcgtg ccgcccaccg
     2281 agatcaacca gccctcggcc tcggcaccgg tgggtgactc gatacccgtg acgtggctgt
     2341 agatgaacgg cgggatgtag gccatcgcct tctcgacgac gtcgcccatc ccgggaaccc
     2401 ggctcagggc accgatcgct tcgacgttgt tgaagtactg cgcgctcaac ggatggatga
     2461 cgaaggcgaa tcggctcttg cgcttgtcgc cgttgggacg caggagccgg ggttcgagtt
     2521 cggcggcctg gatcagctcg aggaggtcgt cggtggtgag ctcccgctcc tcggtgttga
     2581 tggctgcggc catcgcctcg agcatcgcgg tgaccaccat gaactggaag gggcgtggcg
     2641 tcgcgtcgat gatcaggtcg acaccacggc tggtgagctc ggccagtcgg ccatcggaca
     2701 cggcgttggt cacgacggtc ttgccagcga ggtcttcgag cccgaaatgc atcagctcgg
     2761 cgtaactccc gacgatgatg tccgcgtccc gcgcggcccg gtgggcgagt ttgtcgctca
     2821 cccactcgac cggcccctgg ctgtggtcct tgaagaaccc gggggtaagg cgccaggtcc
     2881 acatcccgac ctcggccgcc ttggcgacca ccgggttggt gttgagcgca atggggacgt
     2941 cggtccgcag ggccgggtcg acgaagtcga tgttggccgt gaactcccgc aggacccgga
     3001 tcgtccgctc gtagttcttc ccgctgagaa cgaccgtccg ggcattgttg aagtagccag
     3061 gcatctcggc ttgcacccgg cggatcgccc actcctgcaa cacatcagcg agcatcgacc
     3121 catcgcggac gggcacccgt gcggtcgtct caagaacctg acgcacttcg agagggtcac
     3181 ccttgaagac gccagcggcc tgggcctcac ggacaccggt gacggcgacg gcgtcggcct
     3241 tgagcgacca ctgccacacg agcttctcgg cagcgtcgag gtcgccggtc gtcccgaccc
     3301 ggatgagtcg gaagtcccga ccggcgaagg tcaccttttc gtcgaagtcc cactcggggc
     3361 cctgcagggt gatgttgacg acgacaagcc gtttgcgcgg cataccggat ctcctaggcc
     3421 ttcgagcggg tggggcgacg agacgttgcg ggggctggtg gctcgtcgcg cagttcggcc
     3481 cccgcgacga gtcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtggtg
     3601 gatctgctcg atgagttcgg cgggatcggc gatccccagg ggcatctcga acagcacgac
     3661 ggcgaagtgg ttgcccaacg tcgacggcag gctgccgtcg accggttgca gcgacacggg
     3721 catcatccag gccacgtcgg tgatgtcggt gacgccacgc tcgccgaggt agtccgtcag
     3781 cgccagggac accgccgcca tgagcgcgtc gttcaccgtg ccgttgaagg cctgcgcgac
     3841 cgccttgacc gacgagaggc tgatcccctc ggtccacgcg acgcccttgt cgacgtcggg
     3901 atgcccgctc cacgcgccgg catcggcctt ctcgtgcaac agcagtcggc cgacctcgcg
     3961 ccacgagttg gacgtctcgt tgtcgtccga ggtgaagccg gtgagtacat cgatgagttt
     4021 ggtggggtgc cggaccaggt cgagggcgga ctcaaggtga tgcgggagcg cgagggggtt
     4081 catcgtcgct atgaccgtgc gaccggcctg ggccacgagc gaacccgagt gggtcaccgc
     4141 atcgatggtg tccttgaccg agtgctccgc aaggtgccgg accgcatcga gcgggccatg
     4201 cgatccgccg tcgctgcccc gtcccacggt gtgcggcgtc gcgccgtcgg tggggtcgca
     4261 cgtcccgatg agcaactgca cgaggcgtat gccgtcgccg agcccgtggt ggaagcgggt
     4321 gagcatcgct cccccgccct cgtcctcggg cggccccgag atcaactgca tgtcccacag
     4381 cgggcgagac cggtcgaagg gcaccgagaa ctggctggag acatacgcgc gcagggtctc
     4441 gggctgggag tcggcgagga caacccgcaa cacgtgccgg tccaccgaga agtcgacgtc
     4501 gtcctcccac tcccaatgcc cgtcgtgctg gacggccacc tggctcaaca ggcggtactt
     4561 cgtcaccacg cgctcgaaga tggcgtcgcg caacgcctcc cactcgggaa gctcgtcgaa
     4621 ggtgagcagg ccgtggacat gcatcaggtt gttgggccga tccatcaaca gccaggtgag
     4681 gtcggacgcc gcgatccggg ttcccatcca gagacctttc tcatgagccc atacgtcgtt
     4741 gcgccatgcg gaaagcacgt cgctcgtctg acaccgagtg tggctgtcga gtcgaccgtg
     4801 cgtcacggac tcacgtcccg tccccaggag tgagggatgt cacgtgccgt tggcctgacg
     4861 gggaggccac ccctggtgcc taggctcgat catgaccccc gcttcctccg ggaaccgttc
     4921 ctctgcccac cgtcccggac cgctggaccc ggaacacgtc ctcgccgcga cctatgcgct
     4981 cgatcagagt tacgtgcggg ggctcaccga ccagttggtg gccacgtccg ggacctcgag
     5041 caggctcctc gccccagcca cgggtcagcc gctcggcaac atccccacct cgacacctgc
     5101 cgatgtcctc tcggccatgg cgcgcgcgaa ggaggccgcc aaggactggg ggctctccag
     5161 cttcgccgag cgctccgagg tcctcatgcg cctgcacgag ctgatcctcg atcggcgcga
     5221 ggagatcatg gacctcatct gctgggagtc gggcaagtca cgcatccacg cgttcgacga
     5281 acccctgcac gtcgcgctca cggcgcgcta ctacgcccgg accctcgacc ggcacctggc
     5341 acccaagcgc cgcgacggag tcgtcccgtt cctcacccag atcgaggagc accgcacccc
     5401 caagggcgtc gtggcgatca tctcgccgtg gaactacccg ttcaccatgg cgctcgtcga
     5461 cggcatcgcc gcgatcgccg cggggaacgc cgtcgtctcc aagcccgacc accacactcc
     5521 cctgtcggcc ctgctcggcc tcgcgctgct cgaggaggct gggctcccgg agggtgtctg
     5581 gcagatggtg acgggggcgg gccgggtgct cggcaccccg atgatcgaga acgccgacta
     5641 cgtgtgtttc accggctcga ccgcaacggg ccgggtcatc gcccggcagt gcgccgaccg
     5701 acttatcggc tgctcactcg aactcggcgg caagaacccg attctcgtcc tgcgcgacgc
     5761 cgacctcgac cgggcggccg agggagccgt ccgggccgcc ttctccaacg gtgggcaact
     5821 gtgcgtgtcg accgagcgcc tctatgtcga cgccgaggtt cacgacgagt tcgtcgagaa
     5881 gttcgtcgcg cggaccaagg ccatgcggtt aggagccggc cacgagtggg gcatcgacat
     5941 gggctcgttg atctcggcgg agcagttgac gacggtcgcc gatcacgtcg atgacgccgt
     6001 ccaccacggc gcgcgggtcc tcgccggagg tcgagcccga cccgatctcg gcccctactt
     6061 ctacgagccg accatcctcg acggggtcac ccccgacatg gcctgtcacg ccgacgagac
     6121 gttcggtccg gtcatctcgg tctacccgtt caccaccgag gatgaggcgg tggacctggc
     6181 caacgccggc gactacggtc tcaacgccgc cgtcttcagc cgcgacaaca agcgggcccg
     6241 cctgatcgcc aaccggatca agtgcggcac cgtcaacatc aacgaggcct tcagcgcgac
     6301 cttcgccagc ctcgacgccc ccatgggcgg gatgcgctcc tccggcatcg gccggcgcca
     6361 gggtgccgag ggcatccacc gctacaccga ggtccagtcg gtcgccacgc aatacctgct
     6421 ccgcttcggg ccgcactggg gcctgaccga cgaggcgtat gccgccgtga tgaccggagc
     6481 ggtcaagctc ctcacccgcc tcgggagggc atagtgacca ccgccttcga ttacgacgtc
     6541 gtgatcgtcg gctcgggctt cggaggatcg gtcgccgcgc tacgcctcac cgagaagggc
     6601 tactcggtcg ccgtgctcga agcgggccca cgtttcgagg acgccgacct gcccgacacg
     6661 agcttcgatg tgcgcaacta cctgttcgcg ccgtcgttgg gctgctacgg gatccagcgg
     6721 atcgacctgc tcaacgactg cctcatcctc agcggagccg gggtcggcgg tggctcgctc
     6781 gtctacgcga acaccctcta ccagcctccg gccgccttct acgacgaccc acagtggcgc
     6841 gacatcaccg actggaagac cgagttggcg ccctactacg accaggccac ccggatgctc
     6901 ggcgtcatca ccaacccgtg gcacacgccc tcggacgagg tgatccgcca ggtcgccgag
     6961 gagatgggca agggagacac cttccgctac acgccggtcg gggtgctctt tccgcgcgac
     7021 ggcgagagca agggccagcc cgtcgacgac ccctacttcg gtggcgaagg gccgagacgg
     7081 catacctgca cggggtgtgg cgagtgcatg accggctgcc gccacaacgc gaagaacacc
     7141 ctcgtgaaga actacctcta cctcgccgag aagcatggcg cgcacgtcca cccgctgacg
     7201 accgtgaccc gcgtcgtccc ccgccacggc ggcggatacg acgtccatac gactaacacc
     7261 cgcgcgaaga ccaagcgccg cgcagcgcgt cagagccgca cgtatgccgc gcagcaggtg
     7321 atcttcgcgg cgtcggcact agggactcaa cgcctcctgc acacgatgaa gttcaccggc
     7381 gaactccccc gtctctccga cactctgggc cggttgaccc ggaccaactc cgagtcgatc
     7441 ctcggcgccg gcgcacccaa ggacgacccg cgcgacttca ccgagggtgt ggcgatcacc
     7501 tcgtcgttcc atccggacga gcacacccac atcgagccgg tccgctatgg ccggggctcc
     7561 aacgtcatgt cgctgct
//
LOCUS       DAORPW010000104         7534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2473_length_7534_cov_793.792753, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000104 DAORPW010000000
VERSION     DAORPW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2473_length_7534_cov_793.792753"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="PLB86_08925"
     CDS             <1..1877
                     /locus_tag="PLB86_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRC65296.1"
                     /translation="HQAIEAKEGVEIKNENQTLATITLQNYFRMYSKLAGMTGTAQTE
                     AAELNSIYKLGVITIPTNMPMIRMDQADLIYRTADAKFDAVVEDIVERHAEGQPILVG
                     TVSVEKSEHLSSLLRRRGVRHEVLNAKQHAREASIVAEAGRKGAVTVATNMAGRGTDI
                     MLGGNSEFRAVQELKRRGLDPEETPQEYEDAWDDALAAAEAAVAKEHDEVKSLGGLYV
                     LGTERHESRRIDNQLRGRSGRQGDPGESRFYLSLQDELMLRFNAGMVDRVMQTARIED
                     DVPIESKIVSRSIAGAQSQVEAQNYEIRKNVLKYDDVLNRQRQVIYAERRQVLEGADM
                     EEQIRHFINDVVAAYVNSATAEGYSDTWDLDQLWTALGTLFPVSITVEDVATRAGGRS
                     AVSRDVLLEELTSDAHHAYDQRQELLGEENMREIERRVVMSVLDRKWREHLYEMDYLQ
                     EGIGLRAMAQRDPLVEYQREGFQLFEAMMDGIKEESVGFLFNAPVQIEQTPAPAPAPT
                     PIRVSDMVRNLAASGANPQDASDEDDDLDADDDEGMPSAPADPLAGTAFSRSTPGVSV
                     KGLEATPQATRLHYSAPGETGSVEHREVDAAGGAVPDSGGYRRPTPRRSSKKARRRGK
                     "
     assembly_gap    2027..2126
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2278..3102)
                     /locus_tag="PLB86_08930"
     CDS             complement(2278..3102)
                     /locus_tag="PLB86_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC65297.1"
                     /translation="MSHSPSLPWRAFGVALAGAVGCSLVAVVFVRIGVSSWALASRPG
                     TGRPEDALLALIALLGALLTAWLAMATVLAALAEVPGAVGSACARLSSRITPAVVRRL
                     VTLAVGSALVAATAPATASAVTAATAARHTVVATLDQPPDPTFRPLAEPAPGPAVAVS
                     TPDPGFVPLAPSAPVATATSIDTLERAPRQLPEAGEMHVVRRGDTLWDIAAARLGPGA
                     TAGDIAAAWPRWYAANRRLIGPDPSHIVPGQRLVPPPEGALAGSPAAVLTSPGASR"
     gene            3243..3791
                     /locus_tag="PLB86_08935"
     CDS             3243..3791
                     /locus_tag="PLB86_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65298.1"
                     /translation="MRWERLFDDLEAQAAAEADLELAAEVADRTRRERAVLGLHERLA
                     GMRRVGAISAEVAGLGAVRAVVADVGADWVLLAPTPERSVLVPFGALRSVVGLAGRIA
                     PVGAVAKGFTVGAALRAISRDRAVVTIVDRDGQRRSGTIDAVGQDAVDLAEHPIDLPR
                     RPEHVGDVRTIPFAWIGAVLRV"
     gene            complement(3806..4054)
                     /locus_tag="PLB86_08940"
     CDS             complement(3806..4054)
                     /locus_tag="PLB86_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC65299.1"
                     /translation="MPPRFLQLADVAEILNISSAQAYALVRSGDLPAIKVGGRGQWRV
                     ESEQLEGYIQRMYAATREFVVAHPLGGGTGEDDDADEG"
     gene            4174..4860
                     /locus_tag="PLB86_08945"
     CDS             4174..4860
                     /locus_tag="PLB86_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65300.1"
                     /translation="MSTHGGQTGPGVSVRATRLQRPSWRDSRLIVGIVLVLLATALGA
                     KAVASADDRVPRYAAATTLKPGDRLDAGTIVRVDVLLDDAVAAYLSAQTPPPADSFVL
                     REVRAGELVPVSAVGSRSDVSLQPVTVRVDGDSVAGLISGSVVDVWVSPRDPESAQER
                     YLGAALGLHAVAVAWIPTDQGSFGVSTSSSAVTLLVPAADVQKLIAAQDARSRLTLVP
                     VPGSLHGRGS"
     gene            4857..6329
                     /locus_tag="PLB86_08950"
     CDS             4857..6329
                     /locus_tag="PLB86_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65301.1"
                     /translation="MTPGAAGLPSVLTAVSARWDGPVVAAVEASRQLTIARRCADLAD
                     LLAAAASGLGRVALVSGQLRGLTLSTVATLRDGGVEVVGLADPDDDQGELRLWQLGVR
                     SVVSATAAPHDLVAALLDAAGAGHHAGAGADRSEVGGGWVPESLGEAAGADPILASFA
                     AADIDPIPVRDTLGHVVAVWGPTGSPGRTTVAVNLAVELALLGREVLLIDADTYGGCV
                     AQTLALLDEAPGIATAARAAEQGTLDLAALARLALQVRPGLRVLTGISKAERWPEIRG
                     AALERVLQVCRALVDDVVVDVGFCLEDDEELSYDTQAPRRNQATLTTLQLADAAVVVG
                     GCDPVSLQRLVRGLQELGTVRAPEPIVVVNRVRSGPVGSQPERLVADALERFAGVVQV
                     HTIPDDPAAFDAAMLAGRALAEIVPAAPARRAFLELVGEVVRRAPLPTTSEFEDSAGR
                     SGVSTGPGATRAQGLRRRGSDASGRRGRRHTSRGWGRSRG"
     gene            6374..>7534
                     /locus_tag="PLB86_08955"
     CDS             6374..>7534
                     /locus_tag="PLB86_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRC65302.1"
                     /translation="MADRLTSLDTSFLYMEDRATVMHVGEVMVFDPPGGRLDHERLMG
                     LFAQRIAHLPRYRQKVRWVPGNLANPVWIDDETFDLSYHVRRAALPKPGGDAQLEEFV
                     ARVQPRPLDRGRPLWEAYIVEGLEHGRFAIVTKTHHALVDGAQPLDIGQVLVSDTPGW
                     EKPLPDLWRARHSPSDLSLLTSAVVDSVKGPRALLRSVRGGSQELVDLGGRVVSGVGS
                     LVSTVARGAARPAPTSPLNAHVGQARRFVMVGTDLAAYRAVRRRLSKGPHADDVTVND
                     IVLACIAGALRTWLLTRGSPVHSTTVVRALVPVSVEDESGQGLHQLAACLIDLPVGEA
                     AASMRLHQVAFLMRQQIEGGHAVGADRLVGLAGFAPPTLHAMGARLGSDMSKR"
BASE COUNT         1059 a         2526 c         2738 g         1111 t
ORIGIN      
        1 tgcaccaggc catcgaggcc aaagagggcg tcgagatcaa gaacgagaac cagacgctcg
       61 cgacgatcac cctgcagaac tacttccgga tgtactccaa gctcgccggc atgacgggca
      121 cggcccagac cgaggctgcc gagctcaact cgatctacaa gctgggcgtc atcacgatcc
      181 cgacgaacat gccgatgatc cggatggacc aggccgacct catctaccgc accgccgacg
      241 cgaagttcga cgcggtcgtc gaggacatcg tcgaacggca cgccgagggc cagccgatcc
      301 tcgtcggcac ggtgtcggtc gagaagagcg agcacctgtc gagcctgttg cgccggcgcg
      361 gggtccgcca cgaggtgctc aacgccaagc agcacgcccg tgaggcgtcg atcgtcgccg
      421 aggccggccg taagggcgcc gtcacggtcg ccaccaacat ggccggtcgt ggcaccgaca
      481 tcatgctggg cggcaactcc gagttccggg ccgtccagga gctcaagcgc cgtggcctgg
      541 acccggagga gaccccgcag gagtacgaag acgcgtggga cgacgcgctg gctgcggccg
      601 aagcggccgt cgccaaggag cacgacgagg tcaagtcgct gggcggcctc tacgtccttg
      661 gcaccgagcg gcacgagtcg cggcgcatcg acaaccagtt gcgcggccgg tccggacgtc
      721 agggtgaccc gggggagtcg cggttctacc tgtccctgca ggacgagctc atgctgcggt
      781 tcaacgccgg catggtcgac cgagtcatgc agaccgcccg gatcgaggat gacgtcccga
      841 tcgagtccaa gatcgtcagc cgttcgatcg ccggcgccca gagccaggtc gaggcgcaga
      901 actacgagat ccgcaagaac gtcctcaagt acgacgacgt gctcaaccgg cagcgccagg
      961 tcatttacgc cgagcgccgt caggtgctcg aaggcgccga catggaagag cagatccggc
     1021 acttcatcaa cgacgtcgtc gccgcctatg tcaactcggc cacggccgag ggctactccg
     1081 acacgtggga cctcgaccag ttgtggacgg ccctgggcac cctcttcccc gtgtcgatca
     1141 ccgtcgaaga cgtggccacc cgcgccggtg gccggtcggc ggtctcgcgc gatgtcctcc
     1201 tggaggagct gaccagcgac gcccaccatg cctacgacca gcgccaggag ttgctgggcg
     1261 aggagaacat gcgcgagatc gagcgccgcg tggtcatgtc ggtgctggac cgcaagtggc
     1321 gcgagcacct ctacgagatg gactacctcc aagagggcat cgggctgcgg gccatggctc
     1381 agcgcgaccc gctcgtggaa taccagcgcg aaggcttcca actgttcgaa gcgatgatgg
     1441 acgggatcaa ggaggagtcg gtcggcttcc tcttcaacgc cccggtccag atcgagcaga
     1501 ccccggctcc ggcccccgcg ccgacgccca ttcgggtcag cgacatggtc cgcaacctcg
     1561 ccgcctccgg tgcaaacccg caggacgcca gcgacgagga cgacgacctc gacgccgacg
     1621 acgacgaggg tatgccgtcc gccccggccg acccgttggc cggcacggcg ttctcgcgct
     1681 cgacgccggg tgtcagcgtc aagggtctgg aggccacgcc gcaggcgacc cggctgcact
     1741 actcggcgcc gggggagacc ggatcggtcg agcaccgtga ggtcgatgcg gctggaggtg
     1801 ccgtgcccga ctcgggtggg taccgtcggc cgacgccgcg ccggtcgagc aagaaggctc
     1861 gtcgccgcgg caagtagtcg aggtccggcg agcaactcgc cagcctggtt cggtgcggtc
     1921 agccgaccga caactcggtg acgatccagc gccggtccag gcccgtcagg cgcagggcca
     1981 gggcgcggac ccggtcctgt tggatgacga cggcacacgc ctcgacnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnntcat cgcgggccag gaagtccaag gccaggctgt
     2161 cctggacgaa ttcgctctcc ctgcgggcga agacctcgtc cggaggcacc accgggggcc
     2221 ggctctccgg cagcggcacg acgtggaccc gccgcgggct cgatgcgagg gctgagctca
     2281 tcgcgaggct ccgggcgagg tcagcaccgc cgcgggcgag ccggccaggg caccctcggg
     2341 cggggggacg aggcgttgac cggggacgat gtgcgagggg tcgggcccga tgagccgccg
     2401 attggcggca taccaccgcg gccaggcggc ggcgatgtcg cccgctgtcg cgcccggccc
     2461 gaggcgcgcg gcggcgatgt cccacagggt gtcgcctcga cgcacgacgt gcatctcgcc
     2521 ggcctcgggg agctggcgtg gggcgcgttc gagcgtgtcg atgctcgtcg cggtggcgac
     2581 aggagcgctg ggggccaggg gcacgaagcc tgggtcgggc gtggacaccg ccactgcagg
     2641 gcccggggcc ggctcggcca agggccggaa cgtggggtct ggcggctggt cgagggtggc
     2701 gaccacggta tgccgcgcgg cggtggccgc ggtgacggct gaggcggtcg ccggcgcggt
     2761 tgccgcgacg agtgctgaac cgacggcaag ggtgacgagt cgacggacga cagcaggtgt
     2821 gatgcggctg gacaaccgcg cacaggccga cccgaccgcc cccgggacct cggcgagggc
     2881 ggctagcacc gtcgccatcg ccagccaggc cgtcagcagg gcaccgagca gggcgatcag
     2941 cgccaacaag gcgtcctccg gccggccggt gccaggccgg ctggccagcg cccaactgct
     3001 cacgccgatc cgaacgaaga cgacagcgac gagcgagcag cccacggcgc cggccaaggc
     3061 gacgccgaag gcccgccacg gaagcgacgg tgaatggctc atgggtcccc cagatctcac
     3121 ggttgatgtc gtttgcctat gtttgcctgt gaatgagacc attgtcaaga gtttgcgagg
     3181 gtgtccagag aggcatgtgc tgtcgagacg cgtcggatgg gtgcgggtgg aaggatgacc
     3241 ggatgcgatg ggagcggctc tttgacgacc tggaggccca ggcggctgcc gaagccgacc
     3301 tggagttggc cgccgaggtc gccgaccgca cccgtcgcga acgagcggtg ctggggttgc
     3361 acgagcggtt ggcggggatg cgtcgggtcg gcgccatctc tgcggaggtc gcagggttgg
     3421 gcgcggtgcg cgcagtcgtg gcggacgtcg gggccgactg ggtcttgctg gcgcccactc
     3481 cggagcgttc cgtcctcgtt ccgttcggcg cccttcgatc ggtcgtgggg ctggccgggc
     3541 ggatcgcccc ggtcggtgcg gtcgccaagg ggttcaccgt cggagccgcg ttgcgcgcga
     3601 tcagtcgcga tcgggccgtc gtgaccatcg ttgatcgaga cgggcagcgg cggtcaggga
     3661 cgatcgacgc ggttggtcag gatgccgtcg acctggctga gcaccccatc gatctgcccc
     3721 gtcggcccga gcacgtgggc gatgtgcgaa cgattccgtt cgcctggatc ggcgcagtcc
     3781 tgcgggtctg acgggacggc ttcggtcagc cctcgtcggc gtcgtcgtcc tcgccggtgc
     3841 cgccaccgag gggatgggcg acgacgaact cgcgggtcgc ggcatacatg cgttggatgt
     3901 agccctcgag ctgctcgctc tccacccgcc actgcccgcg gccgcccacc ttgatggccg
     3961 ggaggtcgcc cgagcggacg agcgcatagg cctgggccga tgagatgttg aggatctcgg
     4021 cgacgtcggc gagctgcagg aagcgcggcg gcatgggctc agcataggcg cgtccgccgg
     4081 ccgggaccgg cgtcctccac agggcacccg cagttgtcca cagcctgatc gcggtcggtg
     4141 gaagggaagc cgacaaacca ccacaatggg cgcgtgagca cacatggggg gcaaaccgga
     4201 ccaggcgtga gcgtccgggc gacgcgcttg cagcgcccgt cgtggcgcga cagccgactg
     4261 atcgtcggca tcgtcctggt cctgctggcg accgcgctcg gggccaaggc ggtggccagc
     4321 gccgatgacc gcgtccctcg gtatgccgcg gccaccaccc tcaaacccgg tgatcgcctc
     4381 gacgcgggca cgatcgtgcg cgtggacgtg ctgctcgacg atgcggtggc ggcatacctg
     4441 tcggcccaga ccccgccacc ggccgactcg ttcgtgctgc gtgaggtgcg tgccggtgag
     4501 ctcgtccccg tgtcggccgt gggctcgcgt tcggacgtgt ccctacagcc ggtcaccgtc
     4561 cgggtcgatg gtgactccgt ggccgggctg atctccgggt cggtcgtcga cgtgtgggtg
     4621 agcccacgcg accccgaatc ggcccaggag cgctacctcg gcgcggcgtt ggggctgcac
     4681 gccgtcgcgg tggcctggat cccgaccgat caggggtcgt tcggagtgtc gacgagctcg
     4741 tccgccgtga ccttgttggt gcctgcggcc gacgtccaga aactcatcgc ggcacaggac
     4801 gcccgatcgc ggctcaccct cgtgcccgtg cccggctcgc tgcacggacg gggctcatga
     4861 ccccgggggc cgctggcctg ccgtcggtcc tcacggctgt ctcggcccgg tgggatgggc
     4921 cggtcgtggc tgcggtcgaa gcctctcggc agctcacgat cgcgcgccgg tgtgccgacc
     4981 tggccgatct gctggcggcg gctgcctcag ggctgggccg ggtggcattg gtgtcaggtc
     5041 agttgcgtgg cctgaccctc tcgaccgtcg ccacccttcg tgacggtggc gtcgaggtgg
     5101 tgggtctcgc cgaccccgac gacgatcagg gtgagctgcg gttgtggcag ctcggggtgc
     5161 gctcggtcgt ctctgcgacg gcggccccgc acgacttggt ggcggcgttg ctggacgccg
     5221 cgggggccgg ccaccacgcg ggcgctggtg ctgatcgctc ggaggttggc ggcgggtggg
     5281 tcccagagtc gctcggcgag gcggccggtg cggacccgat cctggcgtcg ttcgccgccg
     5341 ccgacatcga cccgatcccg gtgcgcgaca ccctcggcca cgtggttgcg gtctggggcc
     5401 cgaccggctc gccagggcgc accacggtcg ccgtcaacct cgccgtcgag ctcgctctgc
     5461 tggggcgcga ggtgttgctg atcgacgccg acacctatgg cgggtgcgtc gcgcagaccc
     5521 tcgcgctgct cgacgaagcc ccaggcatcg cgaccgcggc ccgagcggcc gagcagggca
     5581 ccctcgacct cgccgccctc gctcggctgg cgttgcaggt ccgtcccggt ttgcgggtcc
     5641 tcaccgggat atccaaggcc gaacgctggc ccgagatccg cggggccgcg ttggagcggg
     5701 tccttcaggt atgccgcgcg ctcgtcgacg atgtcgtcgt ggacgtcggc ttctgcttgg
     5761 aggacgacga ggaactcagc tacgacacgc aggcgccgcg ccgcaatcag gcgacgctca
     5821 cgacgctgca gctggctgac gccgctgtcg tcgtcggagg ctgcgatccg gtgtccctgc
     5881 agcggctcgt ccgggggttg caggagctcg gcacggtccg agcccccgag cccatcgttg
     5941 tcgtcaaccg cgtccggtcc ggaccggtcg ggtcgcagcc ggagcggctc gtcgcggatg
     6001 cgctcgaacg tttcgccggg gttgtgcagg tgcacaccat ccctgacgat ccggccgcct
     6061 tcgatgccgc catgctcgcc ggtcgggcct tggccgagat cgttcctgca gccccggcca
     6121 ggcgtgcctt cctggaattg gtcggggagg tggtccgtcg ggcacccctg ccgaccacgt
     6181 ccgagttcga ggattcggcg ggtaggtccg gggtctcgac cggcccgggg gccacgcgcg
     6241 cccagggtct tcggcgccgt gggtcagacg cctcggggcg ccggggacgg cggcatacct
     6301 cgcgtgggtg ggggcggtcg cgggggtgag tcggcggcgg ccgggggtcg gagcgatctg
     6361 acaggatggt ccggtggctg accgcttgac ctcgctggac acctcgttcc tttatatgga
     6421 ggaccgggcc acggtcatgc acgtcggcga ggtgatggtc ttcgacccgc cgggtgggcg
     6481 gctggatcat gagcggctga tgggcttgtt cgcgcagcgg atcgcccacc tgccccgtta
     6541 ccggcagaag gtgcgctggg tgccgggcaa cctcgccaat ccggtgtgga tcgacgatga
     6601 gactttcgac ctcagctacc acgtccgccg ggccgcgctg ccgaagcccg gtggcgatgc
     6661 ccagttggag gagttcgtcg cccgggtgca accccggccg ctggaccgag gacgcccgct
     6721 ctgggaggcg tacatcgtcg aagggctcga acacggccgc ttcgccatcg tcaccaagac
     6781 ccaccacgcg ctcgtcgacg gcgctcagcc gctggacatc ggtcaggtgc tggtcagcga
     6841 cacccctggg tgggagaagc cgctgcccga cctgtggcgg gcccggcaca gcccaagcga
     6901 cctcagcctg ctgacctcgg cggtggtcga ctcggtcaag ggcccgcggg ccttgctgcg
     6961 ctcggtgcgc ggcgggtctc aggagctggt ggacctcggt ggacgtgtgg tctccggcgt
     7021 cgggtcgctg gtgtcgaccg tcgcgcgcgg cgcggcgcgt cccgctccca cctcacccct
     7081 caacgcccat gtcggacagg cgcgtcggtt cgtcatggtc ggcaccgacc tcgcggcata
     7141 ccgggccgtc cgtcgccggc tctccaaggg gccgcacgcc gacgacgtga ccgtcaacga
     7201 catcgtgctg gcctgcatcg ccggggcgtt gcgcacctgg ctgctgacgc gcggttcgcc
     7261 ggtccactcg acgacggtgg tccgtgccct cgttccggtc agcgtcgagg acgagtcggg
     7321 ccagggcctg catcaactgg ccgcctgtct gatcgacctg cccgtcggcg aggccgccgc
     7381 gtcgatgcgc ctgcaccagg tcgccttcct catgcgccag cagatagaag gcggccatgc
     7441 cgtcggtgcc gaccggctcg tcgggctggc cggtttcgct ccgccgacgc tgcatgccat
     7501 gggtgctcgg ctgggcagcg acatgtccaa gcgg
//
LOCUS       DAORPW010000105         7517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2482_length_7517_cov_699.817743, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000105 DAORPW010000000
VERSION     DAORPW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2482_length_7517_cov_699.817743"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="PLB86_08960"
     CDS             <1..1325
                     /locus_tag="PLB86_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRC65303.1"
                     /translation="CVKNVKQLHELMGDLLTDDFYADLERDQNERATMSMLVPPQMLN
                     TMVPATGDGDLGMPAAGAAYTAAYYGDPIRRYMLPVFSDRRTDWPSHPKSSRDSLHEH
                     DMWAVEGLTHRYPTKVLAELLPTCPQYCGHCTRMDLVGNSTAQVTKLKFGMKPVDRYQ
                     AMLDYLAATPTVRDVVVSGGDVANLPWKNLESFLDRLLEIDNIRDIRLATKALMALPQ
                     HWGAPDVVEGVGRIAAKAHARGVHVAIHTHVNHVNSVTPLVAETARALMDAGLFNIRN
                     QGVLLRGINDTPKDLLDLSFALQDNASITPYYFYMCDMIPFSEHWRLSLAEAQHLQHA
                     IMGYLPGFATPRIVCDVPFVGKRWVHQVEEYDREHGISYWRKNYRTSIEAADMDALSR
                     EYVYYDPIYTLPEEGQKWWARETNAAAAHAAAFDAAALSRLASAQSLT"
     gene            complement(1291..2778)
                     /locus_tag="PLB86_08965"
     CDS             complement(1291..2778)
                     /locus_tag="PLB86_08965"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRC65304.1"
                     /translation="MSHQRVLIVGSGFSGIAMAARLAQAGIDDFLILEKADDVGGTWR
                     DNRYPGCACDVPSRLYSLSFAPKADWSRDYAPAGEIWDYLRDCVERFGLRSRIVFGAE
                     VTEATWKRGRWEVLAADGREWTAGALVLGVGGLHVPRRPDLPGLGSFAGRVLHTADWP
                     EDDHLSGMRVGVVGTGASAVQLVPSLADRVGALEVFQRTPSWILPRDDRPWSRARQQS
                     YARLPLLGRLARWRTYVRLETRVLGFGRLDRARRLVQEQALARLAEAVPDPATREALT
                     PHYALGCKRVLLSDDYWPTFARPDVALVTAPIDRVEPWGVVTEDGEHHELDALVFATG
                     FDVRGSFDRVRITGLRGRTLADEWASSRSTHLGIEVAGFPELYLLLGPNTALGHNSVL
                     VMIEAAIEHILKALHRADEEGPHVVTRSAQARFGRWVRQRTRHTVWGSGCQSWYLDER
                     GRNVAIWPASSIRYRVATRRLRARSYERVTEDGPVRSTTGPTRVD"
     gene            2905..3390
                     /gene="ybaK"
                     /locus_tag="PLB86_08970"
     CDS             2905..3390
                     /gene="ybaK"
                     /locus_tag="PLB86_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRC65305.1"
                     /translation="MAKASGTPATTALTRAKVAFTVHTYEHDPAAPSYGLEAAAALGV
                     DPARVFKTLLVEADGRLGVGIVAADRLLDLKAIASALGAKKAVMADPAVAERSTGYVV
                     GGISPIGQKRALPTVLDADATAYPTILVSGGRRGLDVELSPADLLALTAGRTAPIARD
                     R"
     gene            3472..4716
                     /locus_tag="PLB86_08975"
     CDS             3472..4716
                     /locus_tag="PLB86_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HRC65306.1"
                     /translation="MIVRRASDRGIVVLLAALTMLGPFTVDTIFPGFPAVQREFTVDA
                     VATQQIVSVYLFTFAVMSLLHGPLSDALGRRPVIVGGLAMFTVTSVLCALAPSMTWLL
                     VGRALQGVFAGGGMIVGRTVVRDVFSGHPAQRAMSQMSMIFGVAPALAPIVGGWLLGW
                     SSWRSIFWFLAIFAVVVLAAVMLLLPETHPPTRRTPLSTRALVSAVIDSSKDPSVRRL
                     LLVSSFNFAALFTYISAAPAFIVDHLHLGPGDFGWLFVPVVTMMIVGSWLTGRLAGRI
                     SGPRFLTLGFGVAVLGGVVQLALDVVGLRTLPWVLVGPVTTGLGIALVFPIVTLVLLE
                     VRPRHRGTLSSMQSFSNTMLNAIVAGALVPLVSASLAGLTGAAVTFSIAGWLVWARHH
                     RMAHPDVSAPPDPESYEPTDRM"
     gene            complement(4793..5254)
                     /locus_tag="PLB86_08980"
     CDS             complement(4793..5254)
                     /locus_tag="PLB86_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRC65307.1"
                     /translation="MEKLDRKLVQLLAADGRMSYTDLGKATGLSTSAVHQRVRRLEER
                     GVVQGYQAVIDPAQVGLPLTAYISLSPLDPAAPDDIPDRIRGFSEIESCHSVAGDFNY
                     ITKVRVATPLDLEDLLTRLRGAAHVQTRTTVVLSTAWEGRPVTGRDTPPKA"
     gene            5379..6398
                     /locus_tag="PLB86_08985"
     CDS             5379..6398
                     /locus_tag="PLB86_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HRC65308.1"
                     /translation="MDQAGRLLLLDSASLYFRAFYGVPDQRTDPSEPPTNAIRGFLDM
                     VATLVDRHRPTHLAACWDNDWRPQFRVQAIPSYKTHRVAAGADPSAPGGVAGAGGAAA
                     SGASGASGDGPSGLTSEEAPDDLAPQVPLIAAALAALGIARVGVDGFEADDVIGALAH
                     RHRGTLAVDIVTGDRDLFQLVDDALGVRVLYTSRGGVRDAEIVDQAVLQERYAVPTGD
                     AYADLAVLRGDASDGLPGVAGIGEKTAAALLATYGSLAGIRAAVDGGDPRIKGAQRTR
                     LEAGAAYLDVAPGVVRVAVDAPLPDVDLRLPTQVADPVLMSRLAVDYGLTGAFNRVLS
                     ALGVG"
     gene            complement(6496..>7517)
                     /locus_tag="PLB86_08990"
     CDS             complement(6496..>7517)
                     /locus_tag="PLB86_08990"
                     /inference="COORDINATES: protein motif:HMM:NF018657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HRC65309.1"
                     /translation="DQLSDHPTEVLPEVPMSAEPRGGKAEMLARGLIGLAKKPVKGIK
                     LQAETAAWAAGKVRKEGLRFLPDSVVRMLPGELSKPVAALANKGRADGDAKAGSLVPS
                     LAPPKSPFNGTVTGNVSFSYHDFAIADLRKVGKLVGGTINDAVLAVTAGALRAYMADH
                     GGIVERPLITSTPISWRTGKEKERFANQVFMLFQPLATHIADPLERLKFARETASAAK
                     ANWDGIPSHLTRRASEWMPVVALGPSMKVMSYLPGTYAPKMFNVSVSNVKGPAVKPVF
                     GGVEIGQYVIFGFLPPGCGLLLGGQSLGDRIIFSATACTDIVTDYRDLPRYLKESLDE
                     LLALA"
BASE COUNT         1081 a         2667 c         2586 g         1183 t
ORIGIN      
        1 actgcgtgaa gaacgtcaag cagttgcatg agctcatggg tgacctgctc accgatgact
       61 tctacgccga cctcgagcgc gaccagaacg agcgggcgac gatgtcgatg ctcgtcccgc
      121 cgcagatgct caacacgatg gtcccagcca cgggcgacgg cgacctcggt atgccggccg
      181 cgggcgcggc atacacggcc gcctactatg gcgacccgat ccggcgctac atgctccccg
      241 tcttctccga ccggcgcacc gactggccga gccaccccaa gtcgtcgcgc gactcgctgc
      301 acgagcacga catgtgggcc gtcgaaggcc tgacccaccg ctacccgacc aaggtgctgg
      361 ccgagttgct gccgacctgc ccgcagtatt gcgggcactg cacccggatg gacctcgtcg
      421 gcaactccac ggcacaggtg accaagctca agttcgggat gaagccggtc gaccgctatc
      481 aggcgatgct cgactacctc gccgcgaccc cgacggtgcg cgatgtcgtg gtctccggcg
      541 gcgacgtcgc caacctgccg tggaagaacc tcgagtcctt cctcgaccgg ctcctggaga
      601 tcgacaacat ccgcgacatc cgcctggcga ccaaggcgct catggctctg ccgcagcact
      661 ggggagcccc cgacgtcgtc gagggcgtcg gtcggatcgc cgccaaggcg cacgcgcgtg
      721 gggtccatgt cgcgatccac acccacgtca accacgtcaa ctcggtcacc ccgttggtgg
      781 ccgagacggc tcgggcgctg atggatgcgg gcctgttcaa catccgcaac cagggcgtgc
      841 tgttgcgcgg gatcaacgac accccgaagg atctgctcga cctgtcgttc gcgctgcagg
      901 acaacgcgtc gatcacgccg tactacttct acatgtgcga catgatcccg ttcagcgagc
      961 actggaggtt gtcgctggcc gaggctcagc acctgcagca cgcgatcatg ggctacctgc
     1021 ctgggttcgc cacgccgcgc atcgtctgcg acgtgccttt cgtcggtaag cgctgggtgc
     1081 accaggtcga ggagtacgac cgggagcacg ggatctccta ctggcgcaag aactaccgca
     1141 cgtccatcga ggccgccgac atggatgcgc tctcgcgcga gtacgtctac tacgacccga
     1201 tctacaccct ccccgaagag ggtcagaagt ggtgggcccg cgagaccaac gccgccgcgg
     1261 cgcacgctgc cgcgttcgac gccgctgccc tcagtcgact cgcgtcggcc cagtcgttga
     1321 cctgaccggc ccgtcctcgg tgacgcgctc gtaggagcgc gcccgaaggc gtcgggtcgc
     1381 cacccggtag cggatcgagg acgccggcca gatggcgacg ttgcggccgc gttcgtcgag
     1441 gtaccagctc tggcaccctg agccccacac cgtgtggcgc gtccgttgcc tgacccagcg
     1501 cccgaaccgg gcctgggcgc tgcgggtgac gacgtgtggg ccctcctcgt cggctcgatg
     1561 cagggccttg aggatgtgct cgatcgcggc ctcgatcatg acgagcaccg agttgtggcc
     1621 gagcgccgtg ttcggcccga gcagcagata gagctcgggg aaaccggcga cctcgatgcc
     1681 caggtgcgtc gagcggctgg acgcccactc gtcggcgagg gtgcgtcccc gaaggccagt
     1741 gatgcggacc cgatcgaaac tcccccgcac gtcgaatccg gtcgcgaaca ccagggcgtc
     1801 gagctcgtgg tgctcgccgt cttcggtgac gaccccccaa ggctcgaccc ggtcgatcgg
     1861 tgcggtcacc aacgcgacgt ccgggcgggc gaaggtcggc cagtagtcgt cggacagcag
     1921 gacccgtttg caccccaacg catagtgcgg ggtcagtgcc tcgcgggtcg ccggatcggg
     1981 gaccgcctct gccaggcgcg ccagggcctg ctcctgcacc aagcgacggg cgcggtccag
     2041 tcgcccgaag cccagcaccc gcgtctccag acgcacatag gtgcgccacc gtgccagccg
     2101 cccgagcagc ggcagccggg cgtagctctg ctggcgggcg cgggaccacg ggcggtcatc
     2161 ccgcggcaag atccacgagg gcgtccgctg gaaaacctcc aacgcgccga cccgatcggc
     2221 caggctcggc acgagttgga cggccgaggc gccggtgccg accacgccga cccgcattcc
     2281 ggagaggtgg tcgtcctcgg gccaatcggc ggtgtgcagc acccgaccgg cgaacgatcc
     2341 gagcccaggc agatccggcc gacgcgggac gtgcagcccg ccgacaccga gcacgagggc
     2401 ccccgcggtc cattcccggc cgtcggccgc cagcacctcc caacggcccc gcttccaggt
     2461 cgcctcggtc acctcggcgc cgaagacgat ccgtgagcgc agtccgaacc gctcgacaca
     2521 gtcgcgcagg tagtcccaga tctcgccggc cggcgcgtag tcgcggctcc agtcggcctt
     2581 cggggcgaaa ctcagtgaat agaggcggct gggcacgtcg caggcgcacc cggggtagcg
     2641 gttgtcgcgc caggtccccc cgacatcgtc cgccttctcc agaatgagga agtcatcgat
     2701 gccggcctgc gccaaccggg cagccatggc gataccggaa aagcctgacc ccacgatgag
     2761 cacccgctgg tgagacacag ccgcagcgta tgccgcctcg gccccggacg tcacactccg
     2821 ggagcagtcc ggcgacagcg gtcgggctgg atacagcagt gccgcggctt cggcgatggg
     2881 cgggagacgg catactcgcg tctcatggcg aaggcgtccg gaaccccggc gaccaccgcg
     2941 ctcacgcggg cgaaggtggc gttcaccgtc cacacctacg agcacgaccc agcggcgccg
     3001 tcctacggcc tggaagccgc tgccgcgctc ggcgtcgacc ccgcgcgggt cttcaagacc
     3061 ctgctcgtcg aggccgacgg gcggctcggc gtcggcatcg tcgccgccga ccggttgctc
     3121 gatctcaagg cgatcgcgtc ggccctcggc gccaagaagg ccgtcatggc cgacccggct
     3181 gtcgccgagc gctcgaccgg ttacgtcgtg ggcggcatct cgcccatcgg gcaaaagcgg
     3241 gcgttgccaa cggtattgga tgccgacgcg acggcatacc cgacgatcct cgtgtccggc
     3301 ggacgccgtg ggctcgacgt ggaactctcc cccgccgacc tactcgccct gaccgcgggc
     3361 cgcaccgcac ccatcgcccg agaccgctga gccgcctagc gcgcgatggg ccggcgagtc
     3421 atcgggtgga ccgctccgcg ccgctgtggc cacgcggcgt agcgtgcttg agtgatcgtc
     3481 cgccgagcat ctgaccgagg aatcgtcgtc ctgctggcgg ccctgaccat gctggggccg
     3541 ttcacggtcg acacgatctt ccctggcttc ccggcggtcc aacgcgagtt caccgtcgat
     3601 gctgtcgcca cccagcagat cgtctcggtc tacctcttca ccttcgccgt gatgagcctg
     3661 ttgcacgggc cgctgtccga cgccctgggc cgacgcccag tcatcgtcgg cgggctggcg
     3721 atgttcacgg tgacctcggt gctctgcgcg ctcgccccct cgatgacctg gctgctcgtc
     3781 ggccgggccc ttcagggggt cttcgccgga ggcggcatga tcgtggggcg caccgtggtc
     3841 cgcgacgtgt tctccggcca cccggcccaa cgcgccatgt cgcagatgtc gatgatcttc
     3901 ggggtggccc cggctctggc ccccatcgtc gggggctggc tgctcggctg gtcgagctgg
     3961 cgcagcatct tctggttcct cgcgatcttc gcggtggttg tcctcgccgc agtgatgctg
     4021 ctgcttcccg agacccaccc gcccactcgt cggaccccgc tgtcgacgcg ggcgctcgtg
     4081 agcgcggtca tcgactccag caaggacccc agcgtgcggc gcctgctgct ggtgtcgtcc
     4141 ttcaacttcg cggcgctgtt cacctacatc tccgcggccc ccgccttcat cgtcgatcac
     4201 ctccatctgg ggccgggcga cttcggctgg ttgttcgtgc ccgtggtgac gatgatgatc
     4261 gtcggatcct ggctgaccgg gcggctggcc gggcggatca gcgggccccg cttcctcacc
     4321 ctcgggttcg gggttgccgt gctgggcggt gtggtccagt tggcactgga cgtggtcggt
     4381 ctgcggactc tcccctgggt gctggtcggt cccgtgacca cgggtttggg gatcgcgctc
     4441 gtcttcccca tcgttaccct cgtgctgctt gaggtgcggc cgcgccatcg agggacgctg
     4501 tcatcgatgc agtcgttcag caacacgatg ctcaacgcca tcgtcgccgg agcactcgtg
     4561 cccctcgtca gcgccagcct tgccgggctg accggcgcgg cggtgacctt cagcatcgcc
     4621 ggatggctgg tgtgggcacg gcatcaccgg atggcgcacc ctgacgtgtc cgcgccgccg
     4681 gaccctgagt cctatgagcc gaccgaccgg atgtagaggc cgcgcggacg tcggctgagc
     4741 ggcgggccgc tgggcggcat acctccgggt gctaggtgcg agagcgctgc ggtcaggcct
     4801 tcggcggggt gtcgcgtccg gtgaccgggc ggccttccca cgcggtggag agcacgacgg
     4861 tcgtgcgcgt ctggacgtga gcggcaccgc gcaggcgggt cagcaggtcc tccaggtcaa
     4921 ggggcgtggc caccctgacc ttggtgatgt agttgaagtc gccggccacc gagtggcacg
     4981 actcgatctc ggagaagccg cggatccggt cggggatgtc gtcgggcgcg gcggggtcga
     5041 gaggcgacag cgagatgtat gccgtgagcg gcagccccac ctgggccggg tcgatgacgg
     5101 cttggtaacc ctgcaccacg ccacgctctt ccagacgccg gacccgctga tgcacggcgg
     5161 aggtcgacag gcccgtcgcc ttgccgaggt ccgtgtagct catccggccg tccgccgcga
     5221 gcagctgcac gagcttccgg tcgagtttct ccatgggggc agcctacgca gtcaacggga
     5281 ccggccgctg gcccgtttgg ggggtgggac gtcgggcctt cgcacggtcg ccgaacgcgg
     5341 ctgtcgcacc cctgcggacc gaactagggt gaggccccat ggaccaggct ggcagactcc
     5401 tcctccttga ctcggcgagc ctgtatttcc gggccttcta tggcgtgccc gatcagcgca
     5461 cggacccgtc cgagcccccg acgaatgcga tccgcggctt cctcgacatg gtggccaccc
     5521 tggtggaccg gcaccggccg acccatctcg ccgcgtgttg ggacaacgac tggcggccgc
     5581 agttccgggt gcaggcgatc ccgtcctaca agacccatcg ggtcgctgcg ggtgccgacc
     5641 cgagcgctcc cggcggcgtg gccggagccg gcggggccgc ggcgtcgggc gcgtcaggcg
     5701 cgtcgggaga tggcccgtcg gggttgacct ccgaagaggc tcccgacgac ctcgcgcccc
     5761 aggtcccgct catcgctgcc gccctggccg ccctggggat cgcgcgcgtg ggggtcgacg
     5821 ggtttgaggc cgacgacgtc atcggggcgc tggcccaccg ccaccggggc accctcgcgg
     5881 tcgacatcgt caccggcgac cgtgacctct tccaactggt ggacgacgcg ctgggtgtgc
     5941 gggtcctcta cacctcccgc ggcggggtgc gcgacgcgga gatcgtggac caggccgtgc
     6001 tgcaggagcg gtatgccgtg cccaccggcg acgcctatgc cgacctcgcc gtcctgcgcg
     6061 gcgatgcgtc cgacgggctc cccggagtgg ccgggatcgg cgagaagaca gcagcggccc
     6121 tgctggcgac ctacgggtcg ctcgcgggca tccgggctgc cgtcgatggc ggcgaccccc
     6181 ggatcaaggg agcccagcgc acccgactgg aggccggggc ggcatacctc gatgtcgctc
     6241 ctggcgtcgt gagggtggcg gtggacgcgc cgcttcccga cgtcgacctg cgcctgccca
     6301 cccaggtggc cgacccggtg ctcatgtcgc gcctggccgt cgactacggc ctcaccggag
     6361 cgttcaaccg ggtgctgtcc gccctcggcg tcggttgatc gagcggggcg agcgggcgct
     6421 gcccttgccg gagcgttagc cacatacgga tgtggccaac gctccgagga tcacgccttc
     6481 ccgctgccgg ttgcctcagg cgagggcgag gagctcgtcc aggctctcct tgagatagcg
     6541 cggcaggtcg cggtagtcgg tgacgatgtc ggtgcaggcc gtcgcgctga aaatgatccg
     6601 gtcgcccagg ctctggccac ccagcagcag accgcagccg ggcgggagga agccgaagat
     6661 gacgtactgg ccgatctcga cgccgccgaa caccggcttg accgccgggc ccttgacgtt
     6721 ggacaccgag acgttgaaca tcttcggcgc gtaggtgccg gggaggtagg acatcacctt
     6781 catgctcggg ccgagggcga cgaccggcat ccactcactg gcgcgccggg tgaggtgact
     6841 cggtatgccg tcccagttcg ccttggccgc gctcgccgtc tcacgggcga acttgaggcg
     6901 ctccagcggg tcggcgatgt gcgtggccaa cggctggaag agcatgaaca cctggttggc
     6961 gaaacgctcc ttctccttgc cggtgcgcca ggagatgggc gtcgacgtga tgagcggccg
     7021 ctcgacgatg ccgccgtggt cggccatgta ggcccgcagc gccccggcgg tgaccgcgag
     7081 aacggcgtcg ttgatcgtgc ctccgacgag cttgccgacc ttgcgcaggt cggcgatcgc
     7141 gaagtcgtga tagctgaagc tcacgttgcc cgtcacggtg ccgttgaacg gtgacttcgg
     7201 cggagccagc gagggcacca acgacccggc cttggcatcc ccgtccgcgc ggcccttgtt
     7261 ggccagggcg gcgaccggct tgctcagctc gcccggcagc atccgcacga cgctgtccgg
     7321 caggaagcgc agcccctcct tgcgcacctt cccagcggcc catgcggcgg tctcggcctg
     7381 caacttgatc cccttgaccg gcttcttggc cagaccgatc agaccgcggg cgagcatctc
     7441 ggccttgccg ccacgcggct cggcgctcat cgggacctcg ggcagcacct cggtggggtg
     7501 gtcggagagt tggtcga
//
LOCUS       DAORPW010000106         7467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2510_length_7467_cov_560.451970, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000106 DAORPW010000000
VERSION     DAORPW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2510_length_7467_cov_560.451970"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLB86_08995"
     CDS             complement(<1..408)
                     /locus_tag="PLB86_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65310.1"
                     /translation="MIRVLQVTPEIGPGTGVGSVAFHLEREWAARGIPTARFTMAEAR
                     GAWLPAAGAPGLRGRLIVATRVVWFSTVGTVLARRAAKRQAPGTVVICHNDALAGDVY
                     VNHGIVLQAMRNRGGAFARVLRNPLHPFTITRDL"
     gene            complement(405..1733)
                     /locus_tag="PLB86_09000"
     CDS             complement(405..1733)
                     /locus_tag="PLB86_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HRC65311.1"
                     /translation="MTTVWRVGQWTLRASGVVVAVGLIAVAGLLVPSQPLVSAGIALG
                     ALALGLTLLDPIALPTLAIPALLVVSRVGAGGTNLAMSDFVLAAAFVPAVLLCQRPVS
                     REFASVIGLNAVYQFATLLTVVANPYLANVVEWFHAWMLVSGALFVGWAIGRGGRGPL
                     AMKLLIAGTIVMAVGTLATALVQYAKGDFGPVYPAWPYPMHKNFAGTVLGLAAVVVYL
                     RPSWLGWSARLANWLLVILLAGVAVTLSRQALLGLVVVFFLVVIWPRVDRRRSRAILV
                     VGAALLGVVGVMVQDQLQSGNRFNSTLQRVDWFQSSATVWLTDPWLGVGLRWWYTDRF
                     EARFQPPNIELELLTSVGVVGLVAFFVMFGGTLRVLRRIDPAYGLLPSMVLICRLAQA
                     QFDLFWAAVLGSLPWLIVGLALGALAHDQAERAENQVRDLVAPKKPGVTR"
     gene            complement(1730..>3096)
                     /locus_tag="PLB86_09005"
     CDS             complement(1730..>3096)
                     /locus_tag="PLB86_09005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC65312.1"
                     /translation="GLAERVSFVGALDTDALADFYRGLDVLAVPSLTTDGWVEQFGRV
                     AVEAMACGIPVVASDSGALPDVVGGAGRLVPPGDAAALGRALVTICDDPTEWASLRAN
                     GFARSQEATWGAVAARYRRLYDLAVHPGHAAHSAHGAHPTEARAVEVIVVAYGTPELL
                     GRALASVAGLPVTVVDNSGSADVERVCRKADVRYLDSGANLGFGGGVNVGLADRLLPG
                     ADVLLLNPDAVLHPGAVAALSARLAADAGLASVGPRQVDETGREARVGWPFPTPARSW
                     IAALGISRALGLVFRRGPGRAGGDGQPGQGAERIDYAIGSVLLLRAEALEQVGGFDED
                     FFLYAEETDWAKRAALLGWRHTVVAEASALHTGAATSVDTAARDTHFAAGLERYLRKH
                     HGAGGWAVARSGEVVGAAVRAVVLPGDRGAAARARLRLLRTGPLRAERALVLSRRRPL
                     VSAP"
     assembly_gap    3097..3196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3197..3478)
                     /locus_tag="PLB86_09010"
     CDS             complement(<3197..3478)
                     /locus_tag="PLB86_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 glycosyl transferase"
                     /protein_id="HRC65313.1"
                     /translation="MNHSAVVDAWRERERLLRHAGLDVQTLSARVWDEAGMPVRLVPR
                     PGEPVEGVATVGRHPALFLFDPRPLWRALGEHWDVIDLHEEPFALATAEV"
     gene            3569..5062
                     /locus_tag="PLB86_09015"
     CDS             3569..5062
                     /locus_tag="PLB86_09015"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRC65314.1"
                     /translation="MGRWVFEFSQAGECINVQLRELLGVLRARWRTVAACLLLVVGLT
                     AAVTVTTTPTYTASARVYLTIDRPADTARGDAGGVSVMHYNDLKTLAEVLYSPTVMEP
                     LHAELGLPPGAGVGVSLNISDTASVMDVVGTSSDPQMAAAIANTTGPVLAKVAVQFSP
                     LLSQSAPNATCVSITKAAIPGLPTSPDVRRNLALGILIGLALGVGLAMVRHAMDTKVR
                     TEADLKVASDRPVLAKIPLEASAVDHPLTIESDPHGAHAESIRRLRTNLMFVDVTTGK
                     HSFVLTSAMPGEGKTTTTINLALAIADAGSRVILVDGDLRNPSVAKALGIEGGVGLTT
                     ALLGRASLAEVIQPWGETGLSILAAGEIPPNPSELLGSRPMAELFEKLAADFDFVLID
                     SPPLVPVIDAVLLQRLTGGLVMVVAAERTRKRDLATALTSLRTVDVAPAGFVLNFVQV
                     DASDSYRYGKYRYQPSQADTNSTPAPRKAPSALGTRASRRQARRAGS"
     gene            complement(5059..5340)
                     /locus_tag="PLB86_09020"
     CDS             complement(5059..5340)
                     /locus_tag="PLB86_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65315.1"
                     /translation="MTHQQHWELAHDTGIVERNGLTYLAALPDGPIVVLDAVASVVLQ
                     VALDVSADDVTSEVAAAYGKVPTEVDAAVSECLSGLERARLLRRVGARG"
     gene            complement(5337..6209)
                     /locus_tag="PLB86_09025"
     CDS             complement(5337..6209)
                     /locus_tag="PLB86_09025"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRC65316.1"
                     /translation="MTNLDAAGSIPPSIRVELAHAAVCNLARRAGVDLLVVKGPGMDP
                     LVGFKGRASADVDVLVRPDEVGRFLDLVKAHGWWARDRFETGSPFGHSTTLFHPTWGD
                     LDVHRLIPGIGVDPSVAFDVFWASRIDVTLGGQPCPHPQPAAQALVLVLHAARSEGSP
                     RAGQDVVHAWTDAPSEQQAAILALVDRLDAHVAWAAGTGDLEAFRGDSSYRLWAVASR
                     GGGRLEEWRARVEAERSWRAKAMIALRAPLVNTDHLAMLLGHRPTRTEVLVEFVDRFR
                     RGAVEMARRGKGRP"
     gene            complement(6206..7345)
                     /locus_tag="PLB86_09030"
     CDS             complement(6206..7345)
                     /locus_tag="PLB86_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65317.1"
                     /translation="MPIPLSGRAHLRFGLFGQTLRLACEPGDLPMLREQWRRLLAPER
                     DEPADREVDEPREGGTTGYPYAVQMRVTLAAIAVGTGRLFMWHAAGLSDPQGRVLGLV
                     AASGTGKTTAAVYLATHGWGYVTDETLACDLDGRVRPYPKPLAFRRPPGDDGALEHKA
                     SVGPDELGLGHPGDELTLHRMVLLDRRQGWSEAPTLTRVPLPEAVRALVGQSSGFVAL
                     DAPLQRLCTVLDQVGGGWRLTYAEIVDAAPALRYLMASPTAPDRTGGQAWRAESPDQP
                     DWRGPVDPQGEIVWRAPYADAVACGNDLTEILVLVGSVPAHVRGIGTDIWRAAGDGVA
                     EADLVGLVEAAHGPHPEAAAMVRAAVDALVQASVLVRGHRRGERR"
BASE COUNT         1258 a         2682 c         2468 g          959 t
ORIGIN      
        1 caggtcacgc gtgatggtga agggatgaag ggggttgcgc aggacccgag cgaacgcgcc
       61 accgcggttg cgcatcgctt gcagcacgat gccatggttg acatagacat ccccagcgag
      121 agcgtcgttg tggcagatga ccaccgtccc tggcgcctgc cgcttcgcag cccgccgcgc
      181 cagcactgtg cccacagtcg aaaaccacac cacacgagtg gcgacgatca gtcggccgcg
      241 cagacccggc gcgccagccg cgggtagcca ggccccccgc gcttccgcca tcgtgaaccg
      301 cgcagtcggt atgccgcgtg cggcccattc tcgttcgagg tgaaacgcca cagagccgac
      361 gcccgttccg gggccaattt ccggtgtgac ttgaaggacg cggatcacct ggtcacccca
      421 ggcttcttgg gagcaacgag gtcgcgcacc tggttctccg ctcgttccgc ctggtcgtgg
      481 gccagcgcac cgagggcgag ccccacgatc aaccacggga gcgatccgag cactgcggcc
      541 cagaacagat cgaactgcgc ctgggctagt cgacagatga gcaccatgga cggaaggaga
      601 ccgtacgcag ggtctatccg gcgcagcacg cggagagtgc cgccgaacat gacgaaaaag
      661 gcaacgagcc cgacgacacc taccgacgtg agcaactcca actcgatatt gggtggttga
      721 aaccgcgcct cgaaccggtc ggtgtaccac catcgcaagc cgactccgag ccacgggtcg
      781 gtcagccaga cagttgcgga ggactggaac cagtccaccc gctgaagcgt cgagttgaag
      841 cggttcccgg actgcagctg gtcctgaacc atgaccccga caaccccaag gagggcagcg
      901 ccgaccacaa ggatcgcccg ggagcggcgg cggtccacgc gtggccagat gacaacgagg
      961 aagaagacaa cgaccaaccc cagcagagcc tgacgcgaaa gagtcacggc gaccccggct
     1021 aacaggatga cgagcagcca gttggccagt cgagcgctcc acccgagcca gctcggccgc
     1081 agatagacga caacggcagc taaccccagg accgtcccgg cgaagttctt gtgcatgggg
     1141 tagggccagg ctggatagac gggcccgaaa tctcctttgg cgtactgcac gagagcagtt
     1201 gccagggttc ccacagccat gacgatggtg ccggcaatca agagcttcat ggccagtggc
     1261 ccacggccac cgcggccgat cgcccacccg acgaacaggg ctccgctgac gagcatccac
     1321 gcgtggaacc actccacgac attggccagg taggggttcg ccacaacggt caggagcgtt
     1381 gcgaattgat agaccgcgtt aagcccgatg acggaagcga actcccgact caccggacgc
     1441 tggcacagca gcactgcggg aacgaaggcg gccgcaagca cgaagtcgga catggccagg
     1501 ttcgttcctc ccgcgccgac acgtgagacc acgaggaggg cggggatggc aagggtcggc
     1561 aaggcgatcg gatcgagcag ggtcagcccc agcgccaacg cgcctaaagc gatgcccgcc
     1621 gacaccaggg gttggctcgg gaccagcagg ccggcaacgg cgatcaggcc cacggcaacg
     1681 accacgcccg acgcgcggag cgtccattgg cccacccgcc acaccgtcgt catggggcac
     1741 tcaccagggg tcggcggcgc gagaggacca gcgcccgttc ggcgcgcagc ggcccagtgc
     1801 ggagcaaccg caaccgcgcg cgtgccgccg ctcctcggtc cccggggagc acgaccgcac
     1861 gcaccgcggc cccgacgacc tcgccggagc gggccacggc ccagcccccc gcgccatggt
     1921 gcttgcgcaa gtagcgttcg agcccggcag caaagtgcgt gtcacgtgcc gccgtgtcaa
     1981 cgctcgtcgc tgctccggtg tgcagggcgc tcgcctcggc gacgacggta tgccgccagc
     2041 ccagcagggc ggcccgcttg gcccagtcgg tctcctcggc atagaggaag aagtcttcgt
     2101 cgaagccgcc cacctgctcg agggcctcgg cccgcagcag caacaccgag ccgatggcgt
     2161 agtcgatgcg ttcggccccc tgccctggct gcccatcgcc cccggcccga ccgggcccac
     2221 gacggaacac gagaccaaga gctcgcgaga tccccagcgc cgcgatccaa ctgcgcgcgg
     2281 gagtcgggaa cggccagccg acccgcgctt cccgtccggt ctcgtccacc tgccggggcc
     2341 cgacgctcgc cagccccgca tcggcggcca gtcgcgccga cagcgcggcc accgcgccgg
     2401 ggtggagcac cgcatcgggg ttgagcagga gcacgtcggc gcctgggagc agccgatcgg
     2461 ccagcccgac gttgacgcct ccgccgaagc cgaggttggc ccccgagtcg aggtatcgga
     2521 cgtcggcctt gcgacagacc cgctcgacgt cggccgaccc ggagttgtcg acgacggtca
     2581 ctggcagacc cgcgaccgag gcgagggccc ggccgagcaa ctcgggggtc ccgtaggcga
     2641 cgacgatgac ctcgacggcc cgagcctcgg tcgggtgggc gccgtgggca gagtgggccg
     2701 catggccagg gtggacggcg aggtcgtaga gccgccggta ccgggccgcg accgcacccc
     2761 aggtcgcctc ttgcgagcgg gcgaatccgt tggcccgcaa ggatgcccac tcggtggggt
     2821 cgtcacagat cgtcaccagg gcgcggccca acgccgccgc gtcccccgga gggaccagcc
     2881 gcccggcccc gccgacaaca tcaggcaggg cgccgctgtc actcgccacc acgggtatgc
     2941 cgcacgccat cgcctcgacg gcgacccgcc cgaactgctc cacccagccg tcagtcgtca
     3001 gcgacgggac ggcgagcacg tccagcccgc gatagaagtc ggccaacgca tccgtgtcga
     3061 gcgcgccgac gaagctcacc cgctcggcca gccccannnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnncacc tcggcggtgg ccagggcgaa cggctcctcg tgcaggtcga
     3241 tgacgtccca atgttccccc agcgcccgcc acagcggccg cgggtcgaag aggaacaggg
     3301 cggggtggcg gccgacggtg gcgaccccct cgaccggctc gccggggcgc ggaaccagac
     3361 ggaccggcat accggcctcg tcccagacgc gcgccgagag cgtctggacg tcgagaccgg
     3421 cgtgccgaag aagccgttcg cgctcgcgcc aggcgtcgac gacggcactg tggttcaccc
     3481 gcagaacccg cacgcatgtc ctccagaccc gtcggctgag gtcagcctag gccgacttcc
     3541 gaggattcca aggtcgttca cggcaggtgt gggacgatgg gtattcgagt tttctcaagc
     3601 cggggagtgc atcaacgtgc agttgcgtga gttgctcggc gttctccgcg cgcggtggag
     3661 gaccgtcgca gcatgtcttc tcctcgtcgt cggcctcacc gccgcggtga cggtgacgac
     3721 gacgccgacc tacacggcgt ccgcacgcgt ctatctcacg atcgatcgac ccgccgacac
     3781 ggcccgcggc gacgccggcg gggtgtcggt catgcactac aacgacctca agaccctcgc
     3841 cgaggtgctc tactctccga ccgtcatgga gccgctgcac gccgagctcg ggctgccgcc
     3901 gggagcgggc gtcggcgtct cgctcaacat ctccgacacc gcctcggtca tggacgttgt
     3961 cggcacctcc agcgacccgc agatggccgc ggcgatcgcc aacaccaccg gccccgtgct
     4021 ggccaaggtc gccgtgcagt tctcgccgct gctgtcccag tcagctccca acgcgacctg
     4081 tgtgagcatc accaaggccg ccattccggg gctcccaacc agccccgacg tgcgccgcaa
     4141 cctggccctt gggatactca tcggcctggc tctcggagtc gggctggcca tggtgcgcca
     4201 cgcgatggac accaaggtcc gcaccgaggc cgacctcaag gtggcatccg accgcccggt
     4261 tctggccaag atcccgctgg aggccagcgc cgtcgaccat ccgctcacca tcgagagcga
     4321 cccgcacggc gcccacgccg agagcatccg ccggctgcgc accaacctca tgttcgtcga
     4381 tgtgacgacc ggcaagcact cgttcgtgct cacctcggcg atgcctggcg agggcaagac
     4441 gacgacgacg atcaacctcg ccctcgcgat cgccgatgcc ggctcccggg tcattcttgt
     4501 cgacggcgat cttcgcaacc cgtcagtcgc taaggcgctc ggcatcgagg gtggcgtcgg
     4561 cctcacgacg gccttgttgg gacgggcctc gctcgccgag gtcatccaac cgtggggcga
     4621 gaccgggctg tcgatcctcg cggccggcga gatcccgccg aaccccagcg agttgctcgg
     4681 gtcgcggccg atggccgagt tgttcgagaa gctggccgct gacttcgact tcgttctcat
     4741 cgactcgccg cccctcgtcc cggtcatcga cgcggtgctc ctgcagcgcc tcaccggggg
     4801 tctggtcatg gtcgtcgctg ccgaacggac ccgcaagagg gacctcgcga ccgccttgac
     4861 ctcactgcgg accgtcgacg tcgcgcccgc gggtttcgtg ctcaacttcg tccaggtgga
     4921 cgccagcgac tcctatcgct atggcaagta ccgctatcag ccgtcgcagg cagacacgaa
     4981 cagcaccccg gctccacgga aggcacccag cgccctgggc acccgcgcct cgcgacggca
     5041 agcccgccgc gccggcagct agccccgcgc gccgacccgt cgcaggagcc tggcccgctc
     5101 cagcccagac aggcactcac tcaccgctgc gtcgacctcc gtgggcacct tcccgtatgc
     5161 cgccgccacc tccgacgtga cgtcgtcggc cgatacgtca agagcgacct gcaagacgac
     5221 gctcgcgacg gcgtcgagga cgacgatcgg gccgtcgggc aaagccgcga ggtaggtgag
     5281 cccgttgcgc tcgacgattc cggtgtcgtg tgccaactcc caatgctgct gatgggtcac
     5341 ggccggccct tgccgcggcg cgccatctcg acggcgccgc gtcggaaacg gtcgacgaac
     5401 tcgaccagga cctcggtgcg cgtcgggcgg tgcccgagaa gcatcgcgag atggtcggtg
     5461 ttgaccagcg gcgcacgcaa cgcgatcatc gccttggccc gccaggaccg ctcggcctcg
     5521 acccgggccc gccactcctc caggcgcccg ccgccacgag aagcgaccgc ccacagccga
     5581 tagctcgaat cgccgcggaa ggcctccagg tcgcccgtgc cggccgccca cgcgacgtgg
     5641 gcatccagtc ggtcgaccaa cgcgaggatc gcggcctgct gctcggacgg ggcgtcggtc
     5701 cacgcgtgga cgacgtcttg accggcgcga ggtgacccct cggaacgggc ggcgtgcagg
     5761 acgagcacga gggcttgggc ggccggctgc ggatgcgggc atggctgacc gcccagcgtc
     5821 acgtcgatcc gggatgccca gaacacatca aaggccacgc tcgggtcgac cccgatgccg
     5881 gggatgagcc gatgcacgtc gaggtcaccc cacgtcgggt ggaagagcgt cgtcgagtgc
     5941 ccgaagggcg agcccgtctc gaagcggtcg cgggcccacc acccatgcgc cttgacgagg
     6001 tcgaggaacc gaccgacctc atcgggccgt acaaggacgt cgacgtccgc tgacgcccgc
     6061 cccttgaacc ccacgagggg atccatgccg ggccccttga cgacgaggag gtcgaccccc
     6121 gctcggcgag cgaggttgca gaccgcggcg tgggccaact cgaccctgat cgagggtgga
     6181 atgctacccg cggcatccag attcgtcatc gacgctcgcc tcgacggtgg ccccggacga
     6241 gcacggaggc ctggacgaga gcatcgacgg ccgcccggac catcgcggct gcttcggggt
     6301 gcgggccatg cgcagcctcg accaggccga cgaggtcggc ctcagccact ccgtcgccgg
     6361 ccgcccgcca gatgtcggtc ccgatgccac gaacgtgggc ggggaccgac ccgacgagca
     6421 cgaggatctc ggtgagatcg ttgccgcagg cgaccgcgtc ggcgtaagga gcccgccaca
     6481 cgatctcgcc ctgcgggtcg acagggccgc gccaatccgg ctgatcgggt gactcggccc
     6541 gccaggcctg gccaccggtg cggtccggcg cggtcggcga cgccatcagg tagcgcagcg
     6601 caggagccgc atcgacgatc tcggcgtagg tcagccgcca cccgccgccg acctggtcga
     6661 gcacggtgca gagccgttgc aggggggcgt cgagggcgac gaagccggag gactgcccga
     6721 ccaaggcccg caccgcttcg ggcaggggca cccgcgtcag ggtcggcgcc tcggaccatc
     6781 cctgccgccg atcgagcagg accatccggt gcagtgtgag ttcgtcgcca gggtggccca
     6841 gacccaactc gtccggccca acgctcgcct tgtgttccag ggccccgtcg tcacccggcg
     6901 gccgacggaa ggccagcggc ttgggatagg gccgcacccg gccgtcgagg tcgcaggcca
     6961 gggtctcatc ggtgacatag ccccagccat gcgtcgccaa gtagaccgcc gctgtcgtct
     7021 ttcccgtccc agacgcagcg accaggccga ggactcgccc ctgcgggtcg gacaacccgg
     7081 cggcatgcca catgaacagt cgacccgtgc ccactgcgat ggcggcgagc gtgacccgca
     7141 tctgcacggc atacgggtag cccgtggtgc cgccctcgcg cggctcgtcg acctcgcggt
     7201 cagcgggctc gtcacgctca ggtgccagca ggcgccgcca ctgctcgcgc agcatcggaa
     7261 ggtcgccggg ctcgcaggcc aggcgcaagg tctgcccaaa caggccaaag cgcaggtgag
     7321 cgcggccgga cagtggaatt ggcacgctcc cgagtatgac gggtggctac atcccggctg
     7381 gacaccgcgg cggcgtcgcg ccgatggtgg cgctctcgac gtacatcacg ttgcaggtcg
     7441 ccgggtcgat gaccgcccac ttgatga
//
LOCUS       DAORPW010000107         7402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2546_length_7402_cov_437.842112, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000107 DAORPW010000000
VERSION     DAORPW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2546_length_7402_cov_437.842112"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLB86_09035"
     CDS             complement(<1..482)
                     /locus_tag="PLB86_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC65318.1"
                     /translation="MGESVLDRFSPATADWFRGSFLAPTPAQEGAWDAISSGRHAVVV
                     APTGSGKTLAAFLWALDRLAVAPVPEDRARRCRVLYISPLKALAVDVERNLRSPLVGI
                     GHAAARLGMPLPQVRVAVRSGDTPAADRRGFVRDPSDILITTPESLFLMLTSAARESL
                     V"
     gene            554..775
                     /locus_tag="PLB86_09040"
     CDS             554..775
                     /locus_tag="PLB86_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HRC65319.1"
                     /translation="MRYSELATMVAQEFGQGYAVSLLRSHVLVALGNRTGDAALADGI
                     PPRQVWAAICDDFDIPAERRFGQDRPKRR"
     gene            complement(802..2097)
                     /locus_tag="PLB86_09045"
     CDS             complement(802..2097)
                     /locus_tag="PLB86_09045"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRC65320.1"
                     /translation="MSRTAKLELDGKSYELPIVEGTEKELAIDISSLRAQTGYITLDD
                     GYGNTGSCSSAITFIDGDKGILRYRGIPIEEIAGQSSFVETAWLVMFGHLPTLDDRER
                     FSKLLTENSRLDESMRKHFEGFPPNGHPMAILSAMINALSTHEPVIIEDERDIEEAAA
                     RLMSKVRTIAAAAYKASVGEPIVYPRYDMKYVENFLHMMFSIPNRDYEPTPEVSRALN
                     LFLSLHADHEQNCSTSTVRMVASSQANMFASCSAGVCALWGPLHGGANVAVIEMLEMI
                     RESGMPVKEYVARVKDKQSGIKLMGFGHRVYRNFDPRSIILKQAADDMLAKMEVHDPL
                     LDIAVELADVALSDPYFVERKLYPNVDFYSGTILRAIGIPLNMFTVMFAIGRMPGWIA
                     NWKEIHDDPKGRIYRPRQVYVGHTGEHWMPREERHYDED"
     gene            complement(2168..4159)
                     /locus_tag="PLB86_09050"
     CDS             complement(2168..4159)
                     /locus_tag="PLB86_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRC65321.1"
                     /translation="MTTTVTLAKRVLVGAPMRSENLGETLLPKRVALPIFSSDALSSV
                     AYATEAILKVLTVGGLAYLYLTPWVALAVALLMTIVVVSYRQVIHAYPDGGGSYEVVR
                     QNLGRGPGMVVAASLMVDYVMTVAVSVAAGVDNVISAVPSLNSQRVLLAVGCVAVLTL
                     VNLRGVRESGRAFAVPTYLFIVGIIAMVGIGLFQTAVGHAPVAVSATYQIREEAFGLT
                     GIALLMLGLRAFSSGCTALTGVEAISNGVPAFQAPKARNAARTLGLMGAIAIVMFVGI
                     TILALVAQVRVAEDTCALVGFAGDCASDPQLTVIAQLAGSVFGADSPAFYFVQGTTAL
                     VLVLAANTAYNGFPMLTSVLARHDLVPRQLRNRGDRLSFSNGIIALAAAAVALLVVYG
                     ADVDQLLHLYVLGVFTSFTLSQIGMVKHWNQQLSEPTTLPSDFARIRLARVVNGVGAV
                     LTAVVLVIVLVTKFTSGAYLVVIAVPLLCLLMREVSRRYARVRRLLASQGNPTRLPSR
                     VHAVVLASTLHEPTLRAVAFARATRPSTLTALTVQVDQDETEALCAAWADAEIPVTLT
                     VVGSPFRDITTPIVDYLQRIRLESPRDLVAVYLPEYVVSHWWEELLHNQSALRLKLRL
                     RLMGNVMLTSVPYVLDGADDPKNTDDGEPTRPRVAPITP"
     gene            4228..6768
                     /locus_tag="PLB86_09055"
     CDS             4228..6768
                     /locus_tag="PLB86_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRC65322.1"
                     /translation="MGPEPRSGLVSEWEPKEASGMPRGRLRVYVGVAPGVGATYAMLA
                     EAARRRARGADVVIGVADTHGRAPLEALMVGSERREPGALDVPALLERRPRIVAVDNL
                     GAIDATTGRARWESVEQLIEAGINVLATTEVRAVASLAEVVAAVTQGPPPPPVPDRIL
                     LGADQVELIDMAPEALRRRLAHGGLRSAGQVDATTASLYSVETLSRLRELGFSWMTGL
                     LAAARPESADPGEVGERILVAVPSGPDAVPLLHRAARLANRAPASELAAVHVVSGRSL
                     PGSADLDLVALRRVAESVGARFHHVVGEDVPQALAEVAMAEHATALVVGGGAPDEGLR
                     RVWGGRHTGALAVRVLDQALGVDVHVVPTASTRSHTRSKPERIPLSRVRVASGTALAV
                     VLPAGLTPLLLMAGDRVGLAGHSLAYLLTVVVVALVGGLWPALVCAVAASTLLNYYFI
                     PPTHTFQVADLHNTVTLVGFLVVAGLVGAIVHRAATMTLAAARASARARTLAAVAEGT
                     IRGEEALPALLEQLRSSFGMTSVSLLAGSDDSHDQSDEPWSVLRSVGPDAPTDPRQSD
                     VQVSAGPGLTLALAGRALVAEERATLRAFAAQVRVVHERDQLSREAAVAERLEATGRL
                     RDALLAAVGHDLRTPLAAATAAVTSLRSADVAWSPSERDELLATAEESLHRLTALVAD
                     LLDLSRLRSGTLTVRAETVWLDDVLGPALDELGVVPGGVQIDIPEGLPPVVADAALLT
                     RALVNVLANALRHSPAGERVIITASPSLGRVEIRVVDRGPGIPAADHARVFQPFQRLG
                     DSDNRTGLGLGLALSRGLVEAMSGALTPEDTPGGGLTMVIGLPEGVTA"
     gene            6765..>7402
                     /locus_tag="PLB86_09060"
     CDS             6765..>7402
                     /locus_tag="PLB86_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC65323.1"
                     /translation="MSRVLIVDDDPQLLRALRINLVARHHDVQVATTGADALQLAAIH
                     LPDLVLLDLGLPDLDGVDVIHGLRGWTDVPIIVLSGRSGGPDKVAALDAGADDYVTKP
                     FGVEELLARMRAVARRGSGAEGGDPVVRFGDLSIDLVNRTVTGPTGAGPSGAGAVSAD
                     SEPIHLTPTEWHVLEVFLRQPGALLSQRYLLGKVWGAAYLNQPANLRLYVSQL"
BASE COUNT         1104 a         2411 c         2729 g         1158 t
ORIGIN      
        1 accagcgact cgcgagccgc ggaggtgagc atgaggaaca gcgactcggg agtggtgatg
       61 aggatgtcgg acgggtcgcg gacgaagccg cgccggtcgg ctgccggggt gtcgccggac
      121 cggacggcga cgcggacctg ggggagcggc atacccaatc gggcggcggc gtggccgatg
      181 ccgacgagcg gggaccgcag gttgcgctcg acgtcgaccg cgagcgcctt gagcggcgag
      241 atgtagagga cccggcagcg gcgggcccgg tcttcgggca ccggcgccac cgccaatcgg
      301 tccagtgccc acaggaaggc cgccaacgtc ttgcccgacc cggtgggcgc gacgacgacg
      361 gcgtggcggc cggaggagat ggcgtcccag gcgccttctt gcgcgggcgt gggcgcgagg
      421 aaggaccccc ggaaccagtc agcggtcgcg ggggagaacc ggtccagcac gctctcgccc
      481 atgtcggggt ccaccctgcc acgacgcacc gacagccgcg ccggtcacgc ggtccacgtg
      541 gcacactcac ccggtgcggt acagcgaatt ggccaccatg gtggcgcagg agttcggcca
      601 ggggtatgcc gtgagcctcc ttcgttcgca tgtcctggtc gccctcggga accgcaccgg
      661 cgacgcggcg ttggcggacg gcatacctcc gcgtcaggtg tgggccgcga tctgtgacga
      721 cttcgacatc ccagcggagc ggcgcttcgg tcaggatcga ccgaagcgcc gctgagggtg
      781 ccgggggact ggctcgaagg gtcagtcctc gtcgtagtgg cgctcttcgc gcggcatcca
      841 gtgctcgccg gtgtggccga cgtagacctg gcgcgggcgg tagatgcggc ccttggggtc
      901 gtcgtggatc tctttccagt tggcgatcca gccgggcatc cggccgatgg cgaacatcac
      961 ggtgaacatg ttcagcggga tgccgatggc gcgcaggatc gtgccggagt agaagtcgac
     1021 gttggggtag agcttgcgct cgacgaagta ggggtcggac agcgccacgt cggccaactc
     1081 gaccgcgatg tccagcagcg ggtcgtggac ctccatcttg gcgagcatgt cgtcggccgc
     1141 ctgcttgagg atgatcgagc gcgggtcgaa gttgcggtag acccggtgac cgaagcccat
     1201 gagcttgatc ccggactgct tgtccttgac ccgggcgacg tactccttca cgggcatccc
     1261 cgactcgcgg atcatctcga gcatctcgat gaccgcgacg ttggcgccac cgtgcagcgg
     1321 cccccagagg gcacagacgc cggccgagca cgaggcgaac atgttggcct ggctggaggc
     1381 gaccatgcgc accgtcgagg tcgagcagtt ctgctcgtgg tcggcgtgca gggagaggaa
     1441 gaggttgagc gcccgagaca cctcgggggt cggctcgtag tcgcggttgg ggatcgagaa
     1501 catcatgtgc aggaagttct cgacgtactt catgtcgtag cgcgggtaga cgatcggctc
     1561 gccgacggag gccttgtatg ccgccgcggc gatcgtccgc accttggaca tgagccgcgc
     1621 ggccgcctct tcgatgtcgc gctcgtcctc gatgatgacc ggctcgtggg tcgagagggc
     1681 gttgatcatc gccgagagga tggccatcgg gtggccgttg ggggggaacc cctcgaagtg
     1741 cttgcgcatg ctctcgtcga ggcggctgtt ctcggtgagg agtttggaga accgctcgcg
     1801 gtcatcgagg gtgggcaggt ggccgaacat gaccagccag gcggtctcga cgaaggacga
     1861 ctgcccggcg atctcctcga tggggatgcc gcggtagcgc aggatgccct tgtcgccgtc
     1921 gatgaaggtg atggccgacg agcacgagcc ggtgttgccg tagccgtcgt cgagggtgat
     1981 gtagccggtc tgggcacgca gtgagctgat gtcgatcgcc agctctttct ccgtgccttc
     2041 gacgatgggc aactcgtagg acttaccgtc gagttcgagc ttggccgtac gggacatcgg
     2101 tgcgcctcca catgccgtct tttgggttgc ctgcatcgta gtcaggaccc ggcgccacct
     2161 agaccggtca gggagtgatc ggcgccaccc tcggccgggt tggctcgccg tcgtcggtgt
     2221 tcttggggtc atccgcgccg tccagcacgt aaggcacgct ggtcagcatg acgtttccca
     2281 tcaaccgcag ccgcaacttg agccgcaggg cgctctggtt gtgcagcaac tcctcccacc
     2341 agtgggagac gacatattcc ggcaggtaga cggcgacgag gtcccgcggg ctctccaggc
     2401 ggatccgctg gaggtagtcg acgatcggcg tggtgatgtc gcggaacggg gagccgacga
     2461 ccgtgagcgt gacggggatc tcggcgtcgg cccaggcggc gcacagagct tcggtctcgt
     2521 cctggtcgac ctgcaccgta agggcggtga gggtcgaagg ccgggtcgcg cgggcgaagg
     2581 ccaccgcacg caaggtcggc tcgtgcaggg tcgaggccaa gacgacggca tgcacccggc
     2641 tcggcaaccg ggtcgggttg ccctgcgatg ccagcagtcg gcgcacccgt gcgtagcggc
     2701 gggacacctc gcgcatgagc agacacagca gcggcaccgc gatcaccacg aggtaggccc
     2761 cgctcgtgaa cttggtgact agcacgatga cgagcaccac agcggtgagc accgcaccga
     2821 ccccattgac gaccctcgcc agacggatcc gtgcgaagtc gctggggagg gtagtcggct
     2881 cgctcagctg ctggttccag tgcttcacca tgccgatctg gctgagcgtg aacgaggtga
     2941 acacgcccag gacgtagagg tgcaggagtt ggtcgacatc ggccccgtac acgacgagca
     3001 gagcgacggc ggctgcggcc agggcgatga tgccgttgct gaaggacagt cggtcgccgc
     3061 ggttgcgcag ttgccggggc acgagatcgt gccgcgccag caccgaggtg agcatcggga
     3121 acccgttgta tgccgtgttc gcggccagga cgaggacgag ggctgtcgtc ccctgcacga
     3181 agtagaaggc cgggctgtcg gcgccgaaga ccgatccggc gagttgggcg atgacggtca
     3241 gttgagggtc ggaggcgcaa tcgccggcga atcccaccag cgcacaggtg tcctcggcga
     3301 cccggacctg agccacgagg gccaggatcg tgatgccgac gaacatgacg atcgcgatgg
     3361 ctcccatgag ccccagcgtc cgcgccgcgt tgcgcgcctt gggcgcttgg aaggcgggca
     3421 ccccgttgct gatggcttcg acgccggtca gggccgtgca cccggaggaa aacgcgcgca
     3481 atccgagcat gagtagggcg atcccggtga ggccgaaggc ctcctcgcgg atctgatagg
     3541 tggcgctgac ggccactggt gcgtggccga ccgcggtctg gaacagcccg atcccgacca
     3601 tggcgatgat cccgacgatg aacagatacg tggggacggc gaacgcccga cccgactcgc
     3661 gcaccccgcg caggttgacc agggtcagca cggcgacgca ccccacggcg agcaggaccc
     3721 gctgggagtt gagcgacggg acggccgaga tgacgttatc gacgccggcc gccaccgaga
     3781 cggccaccgt catgacgtag tcgaccatca gcgacgcggc gacgaccatg ccgggcccgc
     3841 ggccgaggtt ctgccggacg acttcatagg agccgccgcc gtccggatag gcgtggatca
     3901 cctgccggta ggacacgacg acgatcgtca tcaggagggc gaccgccaag gcgacccacg
     3961 gagtgagata gaggtaggcc aaccccccga ccgtgagcac cttgaggatc gcctcggtgg
     4021 cgtaagccac cgacgagagg gcgtcggagg agaagatggg cagggcgacc cgtttgggga
     4081 gcagcgtctc gccaagattt tcgctgcgca tcggagcccc gacgaggacc cgcttggcca
     4141 gggtcaccgt tgttgtcacg tttcgacgct aggtggacgc ccgtgagcgt cgtgcatgga
     4201 acctgctgct tgttgcaagg atcctgcatg ggtcccgaac cacggtccgg gctcgtgtca
     4261 gagtgggaac cgaaggaggc ttccggtatg ccgcgtggcc ggttgcgcgt gtatgtcgga
     4321 gtcgcgcccg gggtcggggc gacctacgcc atgctcgccg aggcggcccg tcgccgtgcc
     4381 cgaggggcgg acgtcgtcat cggcgtcgcc gacacgcatg gccgggcgcc cctggaggcc
     4441 ctcatggtcg ggtccgagcg acgtgagccg ggtgccttgg acgtccctgc gctgctggag
     4501 cgacggcctc ggatcgtcgc cgtcgacaac ctaggcgcga tcgacgcgac gacagggcgc
     4561 gcccgctggg agtcggtcga gcagttgatc gaggcaggga tcaatgtcct tgccacgacc
     4621 gaggtacgtg ctgtggcctc gctggccgaa gtcgtcgccg cggtcaccca ggggccgccg
     4681 cccccaccgg tgccggaccg gatcctgctc ggcgccgacc aggtcgagct gatcgacatg
     4741 gcaccggagg cattacggag gcgactggcg catggggggc tgcgctcggc gggtcaggtc
     4801 gatgccacca cggcgtcgct ctactcggtc gagacgttga gccgcctgcg cgagctcggg
     4861 ttcagctgga tgacgggcct gctcgccgcg gcgcgcccgg agtcggccga ccctggcgag
     4921 gtcggcgaac gcatcctcgt cgcggtgccg tctggcccgg acgcggtgcc cttgcttcat
     4981 cgagccgccc ggctggccaa tcgggcgccc gcgtcggagt tggcggcggt ccacgtcgtc
     5041 agcgggcggt cgttgccggg gtcggccgac ctcgacctgg tcgccttacg ccgggtggcc
     5101 gagagcgtcg gcgcccggtt ccaccacgtc gtcggcgagg acgtcccgca agcgctcgcc
     5161 gaagtcgcca tggctgagca cgcgaccgct ttggtcgtgg gaggcggcgc gcccgatgag
     5221 ggcctacggc gggtctgggg cgggcggcat accggggcgt tggctgtgcg ggtgctggat
     5281 caggcgctcg gggtcgacgt ccatgtcgtg ccgaccgcgt cgacccgctc acacacccgg
     5341 tccaagccgg aacggatccc gctgtctcgg gtccgggtgg ccagtgggac cgcgctggcg
     5401 gtcgtcctcc cggccgggtt gacccccctg ctgctgatgg cgggggaccg ggtcggcctg
     5461 gccggccaca gcttggccta tctgctgacg gtggtcgtgg tcgcgctcgt cggcggcctc
     5521 tggccggccc tggtctgcgc ggtcgcggcg tcgaccctgc tcaactacta cttcatccca
     5581 ccgacccata cgttccaagt ggccgacctg cacaacacgg tgaccctggt cggattcctc
     5641 gtggtggctg ggctcgttgg ggcgatcgtc catcgggccg caacgatgac cttggctgcg
     5701 gcgcgggcgt cggcgcgcgc gcggaccctc gcggccgtgg ccgagggcac gatccgcggc
     5761 gaggaggccc tcccggccct actcgagcaa ttgcgcagca gcttcgggat gacgtcggtg
     5821 agcctgctcg ccggctccga cgacagccac gaccagagtg acgagccgtg gtcggtgctc
     5881 cggtcggtgg ggccggacgc gccgaccgat ccgcggcaga gcgacgtgca ggtgtccgcc
     5941 gggccagggc tcaccctggc cctcgcgggt cgcgctctcg tcgcggagga gcgggcgacc
     6001 ctgcgggcct tcgcggcgca ggtccgggtg gtgcacgagc gtgaccaact gtcccgcgag
     6061 gctgcggtgg ccgagcggct cgaggccacc ggacggctgc gtgacgccct gctggcagcc
     6121 gtcgggcacg atctgcgaac cccgctggcg gcggcgaccg cggcggtgac cagcctgcgc
     6181 agcgctgatg tggcctggtc gccctccgag cgggacgagt tgcttgcgac ggcggaggag
     6241 tcgctgcacc ggttgaccgc attggtcgcc gacctattgg acctgtcccg gctgcgatca
     6301 ggcaccctca ccgtgcgcgc tgagacggtg tggctcgacg acgtcctagg cccggcgctg
     6361 gacgagctcg gggtggtgcc tggtggagtg cagatcgaca tccccgaggg cctgccaccg
     6421 gtcgtggccg acgcggccct gctcacccgc gctctcgtca atgtgctggc gaacgccctt
     6481 cggcacagcc ccgccggcga gcgcgtcatc atcactgcca gcccgtcgct gggacgggtg
     6541 gagatccggg tggtcgaccg cggcccgggg atcccggcgg ccgaccatgc gcgggtcttc
     6601 cagcccttcc agcgactggg cgacagcgac aatcgcaccg gcctcggtct gggattggcc
     6661 ctttcccgag gcttggtcga agcgatgtcc ggcgcgctca cccctgagga cactccaggc
     6721 ggcgggctga cgatggtcat cggcctaccg gaaggggtca cggcatgagt cgggttctca
     6781 tcgtcgacga cgacccgcag ttgcttcgtg ccttgcggat caacctggtc gctcgacacc
     6841 acgacgtcca ggtcgccacc accggcgccg atgcgctgca attggcggcg atccacctgc
     6901 ctgacctcgt gctgctcgac ctcgggctcc ccgatctgga cggggtcgat gtcatccacg
     6961 gcctgcgggg ctggaccgac gtgccgatca tcgtgctgtc gggccggtcc ggggggccgg
     7021 acaaggtggc cgcactcgac gcgggggccg acgactatgt caccaagccg ttcggcgtcg
     7081 aggagttgct ggcccggatg cgggcggtgg cccgtcgcgg gtccggtgcc gagggcggtg
     7141 accccgtcgt caggttcggc gacctgtcga tcgacctggt caaccggacc gtgacggggc
     7201 cgacgggcgc tgggccgtcg ggcgctgggg cggtgagcgc cgactcagag ccgatccacc
     7261 tgacccccac cgaatggcac gtgttggagg tcttcctgcg ccagcccggt gccttgctgt
     7321 cccagcgcta cctcctcggg aaggtgtggg gcgcggcata cctcaatcag ccggcaaacc
     7381 tgcgcctcta tgtctcccaa ct
//
LOCUS       DAORPW010000108         7387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2551_length_7387_cov_744.507092, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000108 DAORPW010000000
VERSION     DAORPW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2551_length_7387_cov_744.507092"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..514)
                     /locus_tag="PLB86_09065"
     CDS             complement(92..514)
                     /locus_tag="PLB86_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRC65324.1"
                     /translation="MVIYLDTSALAKLVVEEAESADLAAWLDARPDTPLVTSTLTRVE
                     LLRAARRRGRSTVPRAIALLAELALIPLDDPVLDAAVLQDPAELRTLEALHTASAALL
                     GAELDALVTYDERMAVAATAVGIPVVSPGSTSAASTPR"
     gene            complement(508..786)
                     /locus_tag="PLB86_09070"
     CDS             complement(508..786)
                     /locus_tag="PLB86_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRC65325.1"
                     /translation="MSTVGIRELRQEASRHVAAAAAGAAVTVTDRGVPVAKLVPLSPL
                     EHHLAQMLARHGIRPPARPRRRFVGDPRLTGPSLTAEILDARAERDAW"
     gene            complement(836..2248)
                     /locus_tag="PLB86_09075"
     CDS             complement(836..2248)
                     /locus_tag="PLB86_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC65326.1"
                     /translation="MKGLLSTLGARVTALTLAVVLATATVGSIAAVLLVQRANDAAAA
                     RSLAATADVAQSLATLATSPELGLARAKRALDGVQVQVAIVRSGTPRGTVVTGDPLPR
                     RLLTEAVLAQLVAGQDVSLRVPDDDGQVFLEGRATEAGAIVLAQRRSDAVALGQDAVA
                     RMRWAFALVALVSVLVGWAVSRRMTTPLRRMAAAAGDLASGARDVAVPESGPAEVAAV
                     GAALNELARSLAHSEARQREFLLSVSHDLRTPLTAVTGYAESLADGVIPPEGVAEVGR
                     IVGAEAARLDRLVQDLLDLARLDAHEVTLTMAAVDLAGLLASAEPVWRRRCDAVGVAF
                     GLELPAVPLVVTADPDRLRQALDGLVDNALRATPVGRPVIVGAHGESAYAVLEVRDGG
                     PGLAPEDFPVAFERSVLHERYRGVREVGTGLGLAIVARIVARHGGTVAARPAPEGGAC
                     FAITLPVATPIVPTTSPVGQ"
     gene            complement(2245..3006)
                     /locus_tag="PLB86_09080"
     CDS             complement(2245..3006)
                     /locus_tag="PLB86_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC65327.1"
                     /translation="MAQPPTPDRRHLVLVVEDEPAIADLVVLHLHHAGFATHVERDGA
                     AAVEAVARHTPAVVILDIGLPGQDGIEVCRALRSAGDWTPVLFLTARDDHVDRVVGLE
                     LGADDYITKPFSPRELVARVQAVLRRSRPLHPDAELLRSGAVECDLAGRRVLVDGAEV
                     PFTATEFDLLTHLITTPGRVFTRTQLLRAAWGEDALLTLAGERTVDVHVAQVRAKLAP
                     HEVIRTVRGVGYAALTDRERADLPGHTDSEPRGRA"
     gene            3127..4389
                     /locus_tag="PLB86_09085"
     CDS             3127..4389
                     /locus_tag="PLB86_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HRC65328.1"
                     /translation="MTSVSVVTDTMGDEQRRRGLRQMRTVALGLLLFAAVVYVLTLGK
                     DGAWGFVNAGAEASMVGAIADWFAVTALFRHPMGLPIPHTALIPKKKDMLARSLQDFM
                     GDNFLREEIIRDRVLAAHISARAAAWALVPANSLRATNEVVGVTIAALNRLSDASVTG
                     LLDEAVLPRLREEPIAPIAGTFLGEVVRDRAHHGLVDLAVSEAHRWLLANEETFAAVL
                     EERAPWWAPTRVNEMVIKRLHVEALAWVEDIRDNPHHHARVALDDLLTQLADDLQHDP
                     ETMERAERIKVRVLDHPQLLQTATSLWAAFRTALITSLGESESPLRARIEGQLRAFAE
                     HVRDEAPLQARLDGYAADLATYFVDRYGDELMTVISHTIERWDGQETAEKVELHVGRD
                     LQFIRINGTIVGGLVGVLIHAVSLLLGR"
     gene            4462..5649
                     /locus_tag="PLB86_09090"
     CDS             4462..5649
                     /locus_tag="PLB86_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC65329.1"
                     /translation="MHLGAYRHVIALPAVRSVLLLGFLVRMPMFATGIVLTLHVVTTL
                     GRSYGEAGLVSAAFTIALAASAPWRGRLLDRHGLRRVVAPSIVATGLVWTTLPFLPYA
                     VLLPASVLGGLFVVPTFSILRQALLAAVPEDRRRTALSLDSVFIELSFMIAPAVAVWA
                     AATYDTQWVLLAVAILNLVAASALYVVNPPITSEAVREVGATVDAPGDKRWVSVGALA
                     VLAAAAATSIVLAGTDVSIVAALRETGDTAAIGWVLSVWGAGSLVGGLVYGAWHRSIS
                     VFWLLAGLAASTVPVALAGSLPWLVVLLFLCGVLCAPTITATLDHLARLVPEGSRGEA
                     MGWHGSAMTAGQAIGAPLAGFAIDHAGWGWGFVSVSLVALAIAVVGGVAERIRRATRL
                     RPA"
     gene            complement(5709..6215)
                     /locus_tag="PLB86_09095"
     CDS             complement(5709..6215)
                     /locus_tag="PLB86_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65330.1"
                     /translation="MAAALVALCATAPAAVADHGGTALSGRPDNAKHYVDRNDLTSAA
                     NAAAVHGVAQLDRSDMDATFTGSGDVEVYDGYYGDIGWYGATNCQTPSTRAGVCDVYR
                     VRFNLTTMDGLSLSKWRSLGCHEFGHTAGLGHRYFYDDSNDNSCMRIEIWPENYDTHD
                     LNAINATV"
     gene            complement(6251..6973)
                     /locus_tag="PLB86_09100"
     CDS             complement(6251..6973)
                     /locus_tag="PLB86_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65331.1"
                     /translation="MTGIRTCAIGLLAGVLLAGCSGSGSTDSTSPQKGSSSAGAAASA
                     PSLSPAAAPDAAPGASAGASAAAPAGGLAAAPSVRAFGKEVLAPESLAAMASASDLVF
                     RGTVTAVAPGRAVGPSASEQIRFREVTIRVDEVVRSTASTPASVLVEEEGWLASGVGY
                     TMNDIGWSAVGDTGYFFLHQKSDRPGAYRLTASNGRALIAGGAVRVSGEDHSPWPQGV
                     GPDQLDRAVRAATKPTTTGGAR"
BASE COUNT         1072 a         2530 c         2638 g         1147 t
ORIGIN      
        1 ccgacgggga gttgcgcgcc cggatcgacg ggatcgcctt cgccgttcgc gccgaatcgc
       61 cggccgcttc gtctgagtga ccctgcgctg actaacgcgg cgtcgaggcc gccgaggtcg
      121 acccggggga caccacaggt atgccgaccg ccgtggctgc caccgccatc cgctcgtcgt
      181 aggtgacgag cgcgtccaac tcggctccga gcagggccgc gctggcggtg tggagggcct
      241 ccaacgtgcg cagctcggct gggtcctgga ggacggccgc atcaaggacc ggatcgtcca
      301 gcgggatgag tgccagctct gccagcaggg cgatggcgcg cggcaccgtc gatcgacccc
      361 gccgtcgggc ggcccgaagc aactcgaccc gagtcagggt gctcgtgacg agaggcgtgt
      421 ctgggcgcgc gtccaaccac gccgcgaggt cagccgattc ggcctcctcc acgaccaact
      481 tcgccaaggc cgacgtgtcg agatagatca ccatgcgtcg cgttcggccc gcgcatccag
      541 gatctcggcc gtcaaactcg ggccggtcaa tcgggggtca ccgacgaagc gtcgacgtgg
      601 ccgagccggt ggccggatgc catgccgtgc cagcatctgg gccaggtggt gttccagagg
      661 ggacaacgga accaacttgg ccaccgggac gcctcgatcg gtcaccgtga cggctgcccc
      721 cgcagccgct gccgcgacat ggcggctggc ttcctggcgc agctcgcgaa tgccgacggt
      781 gctcatctgc cgagagtagc actccatgtg ctacaaagaa ccaccgaggt tggggtcact
      841 gtccaaccgg tgaggtggtg ggaacgatcg gtgtcgcgac gggaagggtg atcgcgaagc
      901 aggcaccacc ctccggcgct ggccgggccg ccaccgtgcc gccgtggcgg gcgacgatcc
      961 gggccacgat cgccagcccg agaccggtcc cgacctcgcg gacccctcga tagcgctcgt
     1021 gcagcaccga tcgctcgaac gccaccggga agtcctccgg cgccaggccg gggccgccgt
     1081 cgcgaacctc gaggacggca tacgcactct cgccgtgagc acccacgatg acgggccgac
     1141 cgacgggggt ggcgcgcagg gcgttgtcga ccaggccgtc gagtgcctgg cgcaagcggt
     1201 cggggtctgc ggtgacgacc agcggcactg cgggaagctc gagcccaaac gcgacgccga
     1261 cggcgtcgca gcggcggcgc cacaccggtt ccgccgacgc gagcagaccc gccaggtcga
     1321 ccgcggccat cgtgagggtg acctcgtgag cgtccaggcg ggccagatcg agcagatcct
     1381 ggacgagccg gtccaggcgc gcggcctcgg ccccgacgat ccgtccgacc tcggccacgc
     1441 cctccggagg gatgacgcca tcggcgagcg actcggcata cccggtgacg gccgtgagcg
     1501 gagtgcgcag gtcgtgcgag acggacagga ggaactcgcg ctgccgggcc tccgagtgcg
     1561 ccagcgatcg ggccaactcg ttgagcgctg cccccacggc ggccacctcg gcagggccgc
     1621 tctccggcac ggcgacatcg cgggcacccg aggcgaggtc gccagcagcc gctgccattc
     1681 ggcgcagggg cgtggtcatg cgacgggaca ccgcccagcc gacgagcact gacaccagcg
     1741 caacgagggc gaacgcccac cgcatccgtg ccacggcgtc ctggccgagc gcgaccgcat
     1801 cgctgcggcg ttgcgccagc acgatagctc cggcctcggt ggcccggccc tcaaggaaga
     1861 cctggccatc gtcgtccgga acccgcaggg acacgtcctg acccgcgacc aactgcgcca
     1921 gcaccgcctc ggtgagcagg cgtcggggga gcgggtctcc ggtcacgacc gtgccccgag
     1981 gggttccgga tcggacgatg gcgacctgaa cctgcacgcc gtccaaggcc cgtttggccc
     2041 gcgccagacc cagctcgggt gaggtcgcga gcgtcgccag cgactgggcg acatcggccg
     2101 tcgcggccag cgaacgggcg gcggccgcgt cgttggcccg ttggacgagc aggactgcgg
     2161 cgatcgagcc cacggtggcc gtcgccagga caacggccag ggtgagcgcc gtgacccgcg
     2221 cgcccagggt cgacagcaga cccttcacgc ccggccccga ggctcggagt cggtgtggcc
     2281 ggggaggtcg gcgcgctcgc ggtcggtcag ggcggcatac ccgactccgc ggacggtgcg
     2341 gatcacctcg tgaggggcga gtttggcgcg cacctgggcg acgtggacat cgacggtgcg
     2401 ctcgccggcg agggtgagca aggcgtcctc gccccaggcg gcccgcagca gttgggtcct
     2461 ggtgaagacc cgcccgggcg tcgtgatcag atgggtgagc aggtcgaatt ctgttgctgt
     2521 gaaaggaacc tcagctccgt cgacgaggac ccgccgcccg gccagatcgc actcgacggc
     2581 gccagagcgc aggagttcgg catcggggtg cagaggtcgg ctgcggcgca gcaccgcctg
     2641 cacccgcgcg accagctcgc gcggcgagaa cggcttggtg atgtagtcgt ccgctcccaa
     2701 ctccagcccg accacccggt cgacgtggtc gtcgcgcgcg gtgaggaaca gcacgggggt
     2761 ccagtcgccg gcggaccgga gcgcgcggca tacctcgatg ccgtcctgcc caggcaagcc
     2821 gatgtcgagg atcaccaccg cgggggtatg ccgtgccacg gcttccacag ccgcagcacc
     2881 gtcgcgctcc acgtgggttg cgaaccccgc atggtgcagg tgcaggacga cgagatcggc
     2941 gatggccggc tcgtcctcga cgacgagcac caggtgccgt cggtcggggg tgggaggctg
     3001 cgccacagcg ccactctagg agccgcggga aggggcgcgg tcacgacggc gtctcggtcg
     3061 tgtcagggtt gtgtcagggc ggttgtgtca gggcgcccgg ttgactggcc gatagaaggg
     3121 acactcatga cgagtgtgag cgtcgtgacc gacacgatgg gcgacgagca gcgccgtcga
     3181 gggctgcggc agatgcgcac cgtggcgttg ggtctgctgc tgttcgccgc ggtcgtctat
     3241 gtcctcaccc tgggcaaaga cggcgcctgg gggttcgtca acgccggcgc cgaggcatcg
     3301 atggtcggcg cgatcgccga ctggttcgcg gtgacggcgc tgttccggca cccgatgggt
     3361 ctgccgatcc cgcacaccgc gctcatcccc aagaagaagg acatgctggc ccggagcctg
     3421 caggacttca tgggtgacaa cttcctgcgg gaggagatca tccgcgaccg ggttctggcc
     3481 gcccacatct cggcgcgggc cgcggcctgg gccctcgtgc cggccaactc ccttcgggcc
     3541 accaacgagg tcgtcggcgt gacgatcgcc gcgctcaacc ggctcagcga cgcatcggtg
     3601 accgggctgc tggacgaggc ggtgctgccg cgactgcgcg aggagccgat cgcgcccatc
     3661 gccggcacct tcctcggcga agtggtgcgc gaccgcgcgc accacgggct ggtcgacctc
     3721 gcggtgagcg aagcccaccg gtggttgctc gccaacgagg agaccttcgc ggcggtcctc
     3781 gaggaacgcg ccccgtggtg ggcgccgacg cgggtcaacg agatggtcat caagcgactg
     3841 cacgtcgaag cgctcgcctg ggtcgaggac atccgcgaca acccgcacca ccatgctcgg
     3901 gtcgccctgg acgacctgct cacccaactc gccgatgacc tccagcacga cccggagacg
     3961 atggagcggg ccgaacggat caaagtccgc gtgcttgacc acccgcagct gctgcagacc
     4021 gcgacctcgc tctgggcggc cttccgcacg gcgctgatca cgtcgttggg ggagagcgag
     4081 tcgccgctgc gcgcgcggat cgaggggcag ttgcgagcgt tcgctgagca cgtgcgcgac
     4141 gaggctcccc tgcaggcccg gctcgacggg tatgccgccg acctcgccac ctacttcgtc
     4201 gaccgctacg gcgatgagtt gatgaccgtc atcagccaca ccatcgagcg ctgggatggc
     4261 caggagacgg ccgagaaggt cgaactccat gtggggcgtg acctgcagtt catccggatc
     4321 aacggcacga tcgtcggcgg gctcgtcggc gtgctcatcc acgcggtgtc gctcctgctg
     4381 ggtcggtgag gtcgtggtga gcgggcggca taccgagtaa ctcggttggg tggcgcgtgc
     4441 cggtggtgag aaactgctct gatgcactta ggcgcctacc gtcacgtcat cgcccttccg
     4501 gcggtgcggt cggtgctgct gttgggcttc cttgtgcgga tgccgatgtt cgccacgggc
     4561 atcgtgctca ccctccatgt ggtcaccacg ctgggtcgtt cctacggcga ggcggggctg
     4621 gtgtccgcgg ccttcaccat cgcgctcgcg gcgtccgccc cctggcgagg gcggctgctg
     4681 gaccgccacg gtctgcgtcg ggtcgtcgcc ccgtcgatcg tcgccacggg cctcgtgtgg
     4741 acgaccctgc cgttcctccc gtatgccgtg ctgcttccgg catcggtgct cggtgggctg
     4801 ttcgtcgtgc cgaccttctc gatcctgcgt caggcgttgc tggccgcggt gccggaggac
     4861 cgccgccgca cggccctctc gctcgactcg gtcttcatcg agttgtcgtt catgatcgct
     4921 ccagccgtgg cggtgtgggc tgccgcgacc tatgacaccc aatgggtcct tctcgcggtg
     4981 gccatcctga acctggtcgc cgcttccgct ctctacgtcg tcaacccgcc gatcacgtcc
     5041 gaagccgtgc gcgaggtcgg ggcgacggtc gacgcgcccg gcgacaagcg ctgggtgagc
     5101 gtcggggcgc tggccgtcct cgcagccgcc gcggcgacga gtatcgtgct ggccggaacc
     5161 gacgtcagca tcgtcgccgc cctgcgtgaa accggggaca ctgccgcgat cggctgggtc
     5221 ttgagcgtgt ggggcgcggg atccttggtc ggtgggctgg tctacggcgc ctggcatcgg
     5281 tcgatctcgg tgttctggtt gttggccgga ctggccgcct ccaccgtgcc ggtcgccctc
     5341 gccggatcgc tgccctggct ggtagtcctg ctcttcctgt gtggcgtgct gtgcgctccc
     5401 accatcaccg ccacgctcga ccacctggcg cggctggtcc ccgagggctc gcgcggggag
     5461 gccatggggt ggcatggatc ggcgatgacc gcgggtcagg cgatcggtgc gccgctcgca
     5521 ggattcgcca tcgaccacgc cggctggggg tggggcttcg tctccgtgtc gctcgtcgcg
     5581 ctggcgatcg ccgtggtcgg tggggtggcc gagcggatcc gtcgggccac gcgccttcga
     5641 ccggcctagg ccgccaggaa acaccgccgc gacggcgccg actctgccgc acgtcgcggc
     5701 ggctcccgct agaccgtggc gttgatcgcg ttgagatcgt gcgtgtcgta gttctccggc
     5761 cagatctcaa tgcgcatgca ggagttgtcg ttgctgtcgt cgtagaagta gcgatgcccc
     5821 aggccggcgg tgtgaccgaa ctcgtgacag ccgagcgagc gccacttcga caacgagagg
     5881 ccgtccatgg tggtcaggtt gaagcgcacc cgataaacgt cgcacacccc ggcccttgtg
     5941 gagggggtct ggcagttggt ggctccatac cagccgatgt cgccgtagta gccgtcgtag
     6001 acctcgacat ctccgcttcc cgtgaaggtc gcatccatgt cgcttcggtc gagctgggct
     6061 acgccgtgga ccgccgccgc gttggctgcc gatgtcaggt cgttacggtc gacatagtgc
     6121 ttggcgttgt cgggtctgcc tgacaacgcc gtgccgccgt ggtcggcgac ggcggcggga
     6181 gcggtcgcgc acagggccac gagtgcggcg gccaccccgc gagagatcct gaccgtcgtc
     6241 aatggggacc tcatcgtgcg cctccggtcg tcgtgggctt ggtggccgca cggaccgcgc
     6301 ggtcgagctg gtcgggtccg acaccttggg gccacggact atggtcctca ccgctgaccc
     6361 ggacggcgcc gccggcaatc aaagctcgcc cgttcgacgc ggtgagccga taggcacctg
     6421 gacggtcgct cttctggtgc aggaagaagt agcccgtgtc gccgacggcg ctccatccga
     6481 tgtcgttcat cgtgtatccg acgccggatg ccagccagcc ctcctcctcg acgaggaccg
     6541 aggccggcgt cgaggcggtg gaccgcacca cctcgtcgac cctgatggtg acctcccgga
     6601 agcgaatctg ctcggaggcg ctcgggccga cggcacgacc gggtgcgacg gccgtgaccg
     6661 tgccccggaa cacgaggtcc gaggcgctcg ccatggcggc gagggactcc ggcgcgagca
     6721 cctccttgcc gaatgcccgg acggatggcg cggcggccag ccctccggca ggtgctgctg
     6781 cggaggctcc ggccgacgcg cctggggcgg catcgggcgc agcggctggg ctcaacgagg
     6841 gcgcagaggc cgctgcccca gcgctcgatg accctttctg cgggctcgta gagtcggtgc
     6901 tgcccgaccc actgcacccg gcgagcagca ccccggccaa caggccgatg gcgcacgtgc
     6961 gtattccggt catgtggtga aaccccctgt ggtctctcgc agaaccgacc acttgcacca
     7021 tcctggaggt ggtcggtttg tccaggtttg gcagccttcc gggtcggcag caggcctggc
     7081 aaggcgcgtc ccatatgtcg gatggagccg gtcgcgggcg gcataccgtg cctgggctgc
     7141 gctgacttat cgtcctgctc ttcgtgtgcg gcttcatctc cgtgtcactc gtcgggcccg
     7201 tggtgacaag agcggctgct cgtcgggtga aacggcgctc cctcagggcg aaaaggactg
     7261 ccccgtcacg gctgaaacgg ctttctcgtc gcgatgaaaa agcctgtcgc acaacgcttt
     7321 atcggtccga aatacttgcc ataggacgtg tgttcgacta gaatcggacc tatgaccacg
     7381 accgtcg
//
LOCUS       DAORPW010000109         7277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2623_length_7277_cov_698.447383, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000109 DAORPW010000000
VERSION     DAORPW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2623_length_7277_cov_698.447383"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLB86_09105"
     CDS             complement(<1..575)
                     /locus_tag="PLB86_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HRC65332.1"
                     /translation="MTIWDDIATERLGIAEVLEGLTEAQWQTPSLCGKWTVRELAGHL
                     VVPFHTSIPNFAVAMVKARGSFHRANDSLARAQSRRPTADLIADLRANANHHFTPPRQ
                     GPLAPLTDALVHGQDLRIPLGINDPRDPDKWQQSLDFLVTPAARAGFTRAELPPVEFV
                     ATDLDWHFGEGPQVSGPAYAIALALTTRAASL"
     gene            659..1768
                     /gene="proB"
                     /locus_tag="PLB86_09110"
     CDS             659..1768
                     /gene="proB"
                     /locus_tag="PLB86_09110"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRC65333.1"
                     /translation="MPRTAVARARRLVVKVGSSSLTTPRGGLDPKRLLALVDALAERH
                     GRDTQLVLVSSGAIAAGMGPLGLARRPRDLATQQAAASVGQGALIAAYQQAFGAHTIT
                     VGQVLLTIDDVTRRTHYLNARRTLERLLDLRVLPVVNENDTVATHEIRFGDNDRLAAL
                     VALLIQADALVLLSDVDALYDGPPSQPGAQRIPIVRGAHDLADVVVAGAGSQVGTGGM
                     ATKVEAAAIANAAGIPTLLTATDRVSDALRGKDVGTVFLPGSRRPPSRLLWLAHATAP
                     HGQLVLDDGAVHAVVTRRKSLLPAGIVRVEGTFVGGDPVDICDLHGKAVARGIVNYGS
                     DELPALLGRSTRDLARELGPEYQREVVHRDALVVL"
     gene            complement(1765..2778)
                     /locus_tag="PLB86_09115"
     CDS             complement(1765..2778)
                     /locus_tag="PLB86_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRC65334.1"
                     /translation="MAHTVSVTTKATTRHTPGVPRTPRVAWQVKFAILVLIWGSSFLF
                     MKVGLEAMTPIQIATMRVLTGMVVVLALLAMTGGRLPTTWRVWRHMIVIGFFLSAFPF
                     TLFAISETRVSSALAGIGNSTTPIATVLATMVLIPNARTSTRKIVAVLIGFVGVILIA
                     QPWTVAERPDLVGFAMAVVGGASYGVGWTYYHRFLSGADLGGLSQPAAVLVVGAGFLV
                     PASLVVWWLDRGHQATPWATHGTADGFQAWLPLLAVIALGALGTGLAFMFQYDVVRAA
                     GPVVGSTITYLIPIVSVLLGTLVLGEHLSWSAVAGFVVVLGAALVINAPERRAESEPV
                     ASA"
     gene            2848..4140
                     /locus_tag="PLB86_09120"
     CDS             2848..4140
                     /locus_tag="PLB86_09120"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRC65335.1"
                     /translation="MVDRTAPDASVTALVAELAGRAKTASRRLALLSRADKDAALHAI
                     ADALDAAIPAVVQANADDLTQGRERGMAEGLLDRLMLDEARIRAIAEAVREVAALPDP
                     VGEVVRGSTLANGLQIRQVRVPMGVVGMIYEARPNVTVDAAALGLKSGNAVILRGGSA
                     AAASNRALVAVIRAALAERGLPEDAVTLLDEGGHDAARALMTARGLVDLVIPRGGADL
                     IRTVVMESTVPVIETGIGVNHVYVDAAADLDKALALVMNSKTHRPSVCNAAESLVVHE
                     SVADEFLPKALTALAGAGVVLRVAPEVSRHAEAAGAAYQLASEADYATEWHRLEMNVR
                     TVADLDEALEHIRVNGSMHTEVIVTESRSAARRFVAEVDAAVVMVNASSRFTDGGQFG
                     MGAEIGISTQKLHARGPMALPELTTTKWIVDGDGQVRA"
     gene            complement(4163..5119)
                     /locus_tag="PLB86_09125"
     CDS             complement(4163..5119)
                     /locus_tag="PLB86_09125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC65336.1"
                     /translation="MTAEPSTERPELSVVIPMFNEQEVLPLLAERLRPVLDGLTDHYE
                     VVCVDDGSSDLTPALLHKLSREWTQVRVIRLRANAGHQAAISAGLARARGDYVVTIDA
                     DLQDPPEVIAEMLQAARRDGVDVVYGVRSDRSTDSVFKRTTARMFYSFIRRISGTTAQ
                     SDAGDFRLMSRATVEAINSLPDTNRVLRFVVPALGFPSATVTYRREERAAGTSKYPFL
                     KMLTLSIDSVTGFSLFPLRLATYLGLIGGVLMFVASIVLVVASRTPGTTIPGWTSTVV
                     IVAGVGAVQLFCLGLLGEYVGRMYTHLQGRPAYFIASDSGAD"
     gene            5295..5489
                     /locus_tag="PLB86_09130"
     CDS             5295..5489
                     /locus_tag="PLB86_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65337.1"
                     /translation="MSASVLPALVRAEGEVHRQLPMPAEMYGVIALVLFVLLLALLWS
                     FRGTAYKVRDRHSKPGGGHH"
     gene            5489..6079
                     /gene="nadD"
                     /locus_tag="PLB86_09135"
     CDS             5489..6079
                     /gene="nadD"
                     /locus_tag="PLB86_09135"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRC65338.1"
                     /translation="MQRIGVMGGTFDPVHHGHLVAASEVQALLSLDEVIFVPTGQPWQ
                     KDGSAVSSAEHRYLMTVIATASNPKFTVSRVDLDRDGPTYTIDTLRDLRAQRPDAELF
                     FITGADALAQILTWKDVDELWSLAHFVGVTRPGHVLSDNGLPTDKVTLLEVPAMAISS
                     TDCRNRVGRGEPVWYLVPDGVVQYIGKHHLYHPRNP"
     gene            6076..6483
                     /gene="rsfS"
                     /locus_tag="PLB86_09140"
     CDS             6076..6483
                     /gene="rsfS"
                     /locus_tag="PLB86_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRC65339.1"
                     /translation="MSATPRAIDLARAAAVGASEKKAENIIAIDVSSQLALTDIFVIA
                     SATNERQVGAIVDEVEERVRLAGAKPLRREGGRDDRWVLIDFGDIVVHIQHQEERAFY
                     QLERLWRDCPTVELGLDEPVSAPAGRSSGDAAD"
     gene            6502..7170
                     /locus_tag="PLB86_09145"
     CDS             6502..7170
                     /locus_tag="PLB86_09145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRC65340.1"
                     /translation="MSGFPSGDTRKLVLVRHGRTDWNLAKRAQGHTDIPLDEVGHAQA
                     QAMAPFVAALRPSRIWSSDLARASQTAAYVAALTGVTVEVDARLREYSVGERSGLTRV
                     EFQERFPREYAAWLAEDETCLVPGAESTADVLARMVPALRSFLAALGPGEVGVVVTHG
                     ACLRAAMLELIGLPLSADAAFATMGNCTWSTLQEAEFGGRLRVAAYNEGVPAHDFASG
                     DEVG"
     gene            7204..7276
                     /locus_tag="PLB86_09150"
     tRNA            7204..7276
                     /locus_tag="PLB86_09150"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7237..7239,aa:Ala,seq:ggc)
BASE COUNT         1152 a         2524 c         2556 g         1045 t
ORIGIN      
        1 agcgaggccg cgcgcgtcgt gagggcgagg gcgatcgcgt atgccggtcc gctcacctga
       61 gggccctcgc cgaagtgcca gtcgagatcg gtggcgacga actcgacagg tggcaactcg
      121 gcccgcgtga atccggcgcg agcggcagga gtgaccagga aatccagcga ctgctgccac
      181 ttgtcggggt cgcgagggtc gttgatacct aaagggattc gcagatcctg cccgtgcacc
      241 aaagcatcgg tcagcggtgc gagtggccct tggcgcggcg gggtgaagtg gtggttggcg
      301 ttggcccgca ggtcggcgat gaggtcggcc gtcggccgcc gcgattgagc ccgagcgagc
      361 gagtcgttgg cccgatggaa gctgcccctc gccttgacca tggccacggc gaagttgggt
      421 atcgacgtat ggaacgggac cacgaggtgg cccgccaact cgcgaacggt ccacttgccg
      481 cacaggctcg gcgtctgcca ctgggcctcg gtcagcccct ccaacacctc ggcgatcccc
      541 aaccgctcag tggcgatgtc gtcccagatg gtcatgctcg cagcctacgg tcttcggtca
      601 tttcgggccg cggatcgcgc ggacgcgcta ggtgccgccc tcgctagact cgtctgccgt
      661 gccccgaacc gccgttgccc gggcccgtcg cctcgtggtc aaggtcgggt ccagctcgct
      721 gaccactccc cgtgggggtc tcgaccccaa gcgcctgctc gccctcgtcg acgccctcgc
      781 cgaacgccac gggcgagaca cccagctcgt cctggtgtct tcgggagcca ttgccgcggg
      841 catgggcccg ctggggttgg ccaggcgccc ccgtgatctg gcgacccagc aggcggcagc
      901 cagcgtgggg cagggagcgc tcatcgcggc ataccaacag gcgttcggtg cccacacgat
      961 cacggtcggt caggtgctgc tcaccatcga tgacgtgacg cgacgcaccc actacctcaa
     1021 cgcgcggcgc acgctggagc ggctgctcga cctgcgggtc ctgccggtcg tcaacgagaa
     1081 cgacaccgtg gccacccacg agatccgctt cggcgacaac gaccggctcg ccgccctggt
     1141 cgccctgctc atccaggccg acgcgctcgt cctgctctcg gacgtcgatg cgctctatga
     1201 cggacctccg agccagcctg gcgcgcaacg gattccgatc gtccgagggg cgcacgacct
     1261 cgccgatgtc gtcgtcgccg gcgctggcag ccaggtgggc accgggggga tggcgaccaa
     1321 ggtggaggcc gcggcgatcg ccaacgcggc cggcataccg actctgctca cggcgaccga
     1381 ccgcgtgtcc gacgcgttgc gcggcaagga cgtggggacg gtcttcctgc cggggtcgcg
     1441 caggccaccg tcgcggctgc tctggttggc ccatgcgacc gctccccatg gtcaactcgt
     1501 gctcgacgac ggcgcggtcc acgcggtcgt cacccgccgc aagtcgttgc tcccagccgg
     1561 cattgtccgg gtcgagggga ccttcgtcgg cggcgacccc gtcgacatct gtgacctgca
     1621 cggcaaggcg gtcgcgcgcg gcatcgtcaa ctacggctcg gacgagttgc ccgcgctgct
     1681 gggccgctcg acccgcgacc tggctcgtga gcttggcccg gagtatcagc gcgaggtcgt
     1741 ccaccgggac gcgctcgtcg tcctctaggc ggacgccacc ggctccgact cggcccgccg
     1801 ctcgggcgcg ttgatcacca gcgccgcgcc cagcaccacg acgaagccag ccaccgccga
     1861 ccagctgagg tgctcgccga ggacgagggt gccgagcagg accgacacga tcgggatgag
     1921 ataggtgatc gtcgacccga cgaccggccc ggcggccctc accacgtcgt actggaacat
     1981 gaacgccaat ccggtcccca gcgcgccgag cgcgatgacg gccagcagcg gcaaccaggc
     2041 ctggaaccca tcggcagtcc cgtgagtcgc ccagggagtc gcctggtgcc cccggtcgag
     2101 ccaccagacc accagcgagg ccgggacgag gaaccctgcg cccaccacca gcaccgcggc
     2161 cggctgcgac agcccgccca gatcggcacc ggagaggaac cggtggtagt aggtccagcc
     2221 gacaccgtag gacgccccac cgacaacggc catcgcgaag cccacgaggt cgggacgctc
     2281 ggcgaccgtc cacggctgcg cgatgaggat gacgccgacg aaaccgatga ggacggcgac
     2341 gatcttgcgg gtcgacgtcc gcgcgttcgg aatgagcacc atcgtggcga gcaccgtcgc
     2401 gatgggcgtc gtcgagttgc cgatgccggc cagcgccgag gagacgcggg tctcggagat
     2461 cgcgaaaagc gtgaacggga acgccgacag gaagaacccg atgacgatca tgtgccgcca
     2521 cacccgccac gtggtcggca gccgcccgcc ggtcatggcc agcagcgcca ggaccaccac
     2581 cattccagtg agcacgcgca tggtggcgat ctggatcgga gtcatcgcct cgagcccgac
     2641 cttcatgaac aggaagctcg acccccagat gaggacaagg atcgcgaact tgacctgcca
     2701 cgccacccgg ggggtgcgtg gaaccccagg ggtatgccgt gtggtcgcct tcgtcgtcac
     2761 cgagacagtg tgagccatac cgggacaacg ctgcgcaccc gtccgatgtg agcgggaggt
     2821 gggcgagggc ggcataccct tggacgcatg gttgaccgca ccgcgcccga cgcctccgtg
     2881 accgctctcg ttgccgagtt ggccgggcgc gccaagaccg cctcgcgccg tctcgcgctg
     2941 ctctcgcgcg ccgacaagga cgccgcgctg cacgccatcg ccgacgcgct cgacgcggcg
     3001 atcccggcgg ttgtccaggc caacgctgac gacctgaccc aagggcgcga gcggggcatg
     3061 gccgagggac tgctcgaccg gctcatgctc gacgaggccc ggatccgggc gatcgccgag
     3121 gcggtccgtg aggtcgccgc gctgcccgac ccggtcggcg aggtggtccg cggctcgacc
     3181 ctggccaacg gcctgcagat ccgccaggtg cgggtgccga tgggagtcgt ggggatgatc
     3241 tacgaggcgc gccccaacgt cacggtcgac gccgccgcgc ttgggctcaa gtcgggcaac
     3301 gccgtcatcc tgcgcggtgg ctcggcggcc gccgcgagca accgggcgct cgtcgccgtc
     3361 atccgcgcag ccctggccga gcgcggcctc cccgaggacg ccgtcaccct gctcgacgaa
     3421 ggcggccacg acgccgctcg cgcgctcatg accgcgcgcg gactggtcga cctggtcatc
     3481 ccgcgcggcg gcgccgacct catccgcacc gtcgtcatgg agtcgaccgt ccccgtcatt
     3541 gagaccggga tcggcgtcaa ccacgtctat gtcgacgcgg ccgccgacct cgacaaggcg
     3601 ctcgcgctgg tgatgaactc caagacgcac cggcccagcg tgtgcaacgc cgccgagtcc
     3661 ctcgtggtcc acgagtcggt ggccgacgag ttcctgccca aggctctcac tgcgctcgcg
     3721 ggcgccgggg tggtcctgcg cgtcgcgccc gaggtgagcc gacatgcgga ggcggcgggc
     3781 gcggcatacc aactggcgtc cgaagcggac tacgcgaccg agtggcaccg gctggagatg
     3841 aacgtccgca cggtcgcgga tctcgacgag gcgctggagc acatccgggt caacgggtcg
     3901 atgcacaccg aggtgatcgt caccgaaagt cgttcggcgg caaggcggtt cgtcgccgag
     3961 gttgacgcag cggtggtcat ggtcaacgcg tccagccgct tcaccgacgg cgggcagttc
     4021 gggatggggg ccgagatcgg catctccacc cagaagctgc acgcccgcgg cccgatggcg
     4081 ctgcccgagc tcacgacgac caagtggatc gtcgacggcg acggccaggt ccgcgcgtag
     4141 cggtcagtcg gcgccggagc gctcagtcgg cgcccgagtc agaggcgatg aagtatgccg
     4201 gtcgcccctg caggtgggta tacatccgtc cgacgtactc accgagcaga cccagacaga
     4261 acagctgaac cgcgccgacc cccgcaacga tgacgacggt cgaggtccaa ccggggatgg
     4321 tcgtgcccgg tgtccgcgac gcgaccacca ggacgatcga ggcgacgaac atgaggacgc
     4381 cgccgatcaa cccgagatag gtcgcgaggc gcagcgggaa aagggagaag ccggtcactg
     4441 agtcgatcga cagggtgagc atcttgagga acgggtactt cgacgtgccc gcggcccgct
     4501 cctcgcgtcg ataggtgacc gtcgccgacg ggaaacccag cgccggcacg acaaaccgca
     4561 gcacccggtt ggtgtcagga aggctgttga tggcctcgac ggtggcccgc gacatgaggc
     4621 ggaagtcgcc cgcatcggac tgcgccgtcg tgcccgagat ccgccggatg aaggagtaga
     4681 acatccgcgc cgtggtccgc ttgaagaccg agtcggtgga tcgatcagag cgcacgccat
     4741 agacgacgtc gaccccgtcg cgccgggcgg cctggagcat ttcggcgatc acttccgggg
     4801 ggtcctgcag gtcggcgtcg atcgtcacga cgtagtcgcc gcgcgctcga gccagccccg
     4861 ccgagatcgc cgcctggtgc ccggcgttgg cgcgcagccg gatcacccgc acctgggtcc
     4921 actcgcgaga cagcttgtgc agcagggccg gcgtgaggtc gctgctgccg tcatcgacgc
     4981 agacgacctc gtagtgatcg gtgagcccgt cgagcaccgg tcgcaggcgc tccgcgagca
     5041 gcggcagcac ttcctgctcg ttgaacatgg ggatgaccac cgagagttcg gggcgctcgg
     5101 tgctcggttc cgcggtcatg gtgccaaggg tacgggccgg gacctggccc acctgcgagg
     5161 ccgctgtgca ggccccatga agggcagtcc gcgggcgcgc ggacgggggc aggcggcata
     5221 cctcacacgc gtggtgacag ccaagaaggt atgccgcgcg cacggggagg ggggtcaccc
     5281 gctagcatgt ggccatgtcc gcgagcgtgc ttcccgccct tgtccgtgcg gagggcgagg
     5341 tccaccgcca gttgccgatg ccggcagaga tgtacggcgt catcgccctg gtgctgttcg
     5401 tgctcctgct cgcgctgttg tggtccttcc gcggcaccgc ctacaaggtg cgcgaccgcc
     5461 actccaagcc cggcgggggc caccactgat ccagcggatc ggggtgatgg gcgggacgtt
     5521 cgatcccgtc caccacggtc acctggttgc cgcgagtgag gtccaggccc tgctgagcct
     5581 cgacgaagtc atcttcgtcc ccaccggtca gccatggcag aaggacgggt cggcggtgtc
     5641 gtcggccgaa catcgctatc tcatgacggt cattgcgacc gcctcgaacc cgaagttcac
     5701 cgtcagccgg gttgatctgg accgggacgg tccgacctac acgatcgaca ccctgcgcga
     5761 tctgcgcgcc cagcggcccg atgccgagct gttcttcatc accggcgccg acgccctcgc
     5821 ccagatcctc acctggaagg acgtcgacga gctctggtcg ctcgcccatt tcgtcggggt
     5881 cacccggccg gggcacgtgc tcagcgacaa cggcctgccg accgacaaag tcaccctgtt
     5941 ggaggtgccg gccatggcca tctcgtccac cgactgccgc aaccgggtcg gccgcggcga
     6001 gcccgtgtgg tacctcgtgc ccgatggcgt ggtccaatac atcgggaagc accacctcta
     6061 ccaccctagg aacccgtgag cgccacaccc cgagccatcg acctcgcccg agccgcagca
     6121 gtcggcgcgt ccgagaagaa ggccgagaac atcatcgcca tcgacgtctc cagtcagctg
     6181 gccctcaccg acatcttcgt catcgcctcg gcgaccaacg aacgccaggt cggtgcgatc
     6241 gtcgacgagg tcgaggagcg tgtccggctg gccggcgcca agccgctgcg tcgcgagggc
     6301 gggcgtgacg accgctgggt cctcatcgac ttcggcgaca tcgtcgtgca catccagcac
     6361 caggaggagc gcgccttcta ccaactcgag cggttgtggc gggactgccc gaccgtggag
     6421 ttggggctcg acgagccggt ctcggcgcct gcagggcgtt cgagcggcga cgccgccgac
     6481 tgaccggctg tgccgacccg gatgagcggg ttcccgtcgg gggacacgcg caaacttgtg
     6541 ctggtgcgcc acggccggac cgactggaac ctcgccaagc gtgcccaggg ccataccgac
     6601 atccccctcg atgaggtcgg gcacgcgcag gcacaggcga tggcaccgtt cgtcgcggcc
     6661 ctgcgcccca gccggatctg gtcctcggac ctcgcgcgcg cgagtcagac cgcggcatac
     6721 gtggcggcgc tgaccggtgt gaccgtggag gtggacgcgc gactgcggga gtactccgtg
     6781 ggggagcgct ccgggctgac gcgcgtcgag ttccaggagc ggttcccgcg ggagtatgcc
     6841 gcgtggctgg ctgaggacga gacgtgcctg gtcccggggg ccgagtccac cgccgacgtg
     6901 ctggcccgga tggtgcctgc gctgcggtcg ttcttggcgg cgttggggcc tggtgaggtc
     6961 ggtgtggtcg tcacccacgg ggcgtgcctg cgagcggcga tgctggagct gatcgggctg
     7021 ccgctgagcg ccgatgcggc gttcgcgacg atgggcaact gcacgtggag cacgttgcag
     7081 gaggccgagt tcggcggccg gctgcgggtc gcggcctaca acgagggcgt gccggcacac
     7141 gatttcgcgt ccggcgacga ggttgggtag gattcacggg ttgcccggtg cgagccgggg
     7201 agcggggctg tagcgcagtt ggtagcgcac ctccatggca tggagggggt caggggttcg
     7261 aatcccctca gctccac
//
LOCUS       DAORPW010000110         7248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2636_length_7248_cov_689.739052, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000110 DAORPW010000000
VERSION     DAORPW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2636_length_7248_cov_689.739052"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1182
                     /gene="tdh"
                     /locus_tag="PLB86_09155"
     CDS             136..1182
                     /gene="tdh"
                     /locus_tag="PLB86_09155"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HRC65341.1"
                     /translation="MRALTKVNAGPGLELVDRPEPTCGPLDVKVRVLRAGLCGTDLHL
                     EQWDDWAASTVKAPMVIGHEFYGEIVEVGEDVPAGTAVAVGARVSGEGHIVCGHCRNC
                     RAGRRHVCINTIGIGVNRDGAFADFIVLPATNVWVQPDSIDPDLGAIFDPLGNATHTA
                     LQWPLVGEDVLITGAGPIGVIAAAIARHAGAKNVVVTDISDYRLGLAKAAGADLTVNV
                     ATTRIKDAQRELGMREGFDIGLEMSGAPSAVAEMLANLNHGAKVAMLGLPKDPYPIDW
                     GRVITHMITLKGIYGREMFETWYLMSSMLATSETFTSAVSSVITHRFPASQWADAFAA
                     ARSGECGKVVLNWS"
     gene            1248..1781
                     /locus_tag="PLB86_09160"
     CDS             1248..1781
                     /locus_tag="PLB86_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC65342.1"
                     /translation="MTPSDDLMDAALAVVGEAGVPGLTWKRIDERAGAAEGATFSLYP
                     TMDLLVEAMLGRLITLDGQAWLATGTALNPTSAREFADRMAALTVSHIELMGQATRAR
                     FALFLGRPQAMAQGQAALLDVITVILDSIGVPDPAARAQHVVDLVGGTALHALTVRQG
                     EPIDEAALAAAFWRLLR"
     gene            1821..3020
                     /locus_tag="PLB86_09165"
     CDS             1821..3020
                     /locus_tag="PLB86_09165"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRC65343.1"
                     /translation="MYGSIKDELLATLAEIDAAGLYKRERQLTTAQSAHVGVGGGSPA
                     VLNFCANNYLGLADHPDVVAAAKAALDEWGFGMASVRFICGTQTQHTLLEDRLSAFLG
                     TEATILYSSCFDANGGVFEVLFGEGDAIISDELNHASIIDGVRLAKAARYRYRNADMA
                     DLRVQLEAARAAGARRVVIVTDGVFSMDGSYAPLPQICDLAEEYAAMVLVDDSHAVGF
                     VGEGGRGTPELFGVADRVDIVTGTLGKALGGASGGYVSSHREVVDLLRQRSRPYLFSN
                     SVAPAVVAGSLAALDLVASSGEARAALRANSTLFRELMTAAGFDLLPGSHPITPVMFP
                     GEDGARQATMIADHMLGNGVYVIAFSFPVVPKGKARIRVQLSAAHSEADVRACVAAFV
                     AARDAVG"
     gene            complement(3041..4168)
                     /locus_tag="PLB86_09170"
     CDS             complement(3041..4168)
                     /locus_tag="PLB86_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann fold nucleotide-binding protein"
                     /protein_id="HRC65344.1"
                     /translation="MEIDRLEQLRALLARGGALGGVRLQDLDLHAVEEQLLDRTDVTD
                     LVVLGGRLSPALTLHLIRHGAIVFPSDPKAPVEVYRARLYTADELYAGLSGPLGYAGT
                     PDARAFDWTMDRRSGRDAYVTMLRAIHDESMTDTLDEVLDGLPVVGVMGGHEAQRGSG
                     VYAAAARLGHLLAEGGCVVATGGGPGAMEAANLGAYAASPADLDAAIAALSKVPSYRP
                     DVGPWAELGLRVREQLGRRPDSGELRGLPLRSIGIPTWFYGHEPPNVFGQGIAKYFSN
                     ALREDGLLARCTAGTVVLPGAAGTVQEIFQGVTRLYYGRLADPTGTLPPLVLVGRSHW
                     GRLVPTWPLVTALAGNRPMADVVHLVDTIEEAAAILLPSAD"
     gene            4302..6203
                     /locus_tag="PLB86_09175"
     CDS             4302..6203
                     /locus_tag="PLB86_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HRC65345.1"
                     /translation="MSTGAYSTAYAASLANPTAFWGEAAKDIDWITPPTTVLDDSRPP
                     FYRWFPDGTLNTCFNAIDRHVAGGRADQVAIHYDSPVTGRKDSFTYAELLAHVSRLAG
                     ALVGAGVGKGDRVLVYMPMVPEAVFAMLACARIGAIHSVVFGGFAPAEVAARIDDAKP
                     KLILSASCGIEPNRVVPYKPFLDEGIARSEHKPDQVVVLQREQARAELGERDVDWAEF
                     ISTPAADAGADCVEVAATDPLYILYTSGTTGKPKGIYRDNGGHAVALRWSMRNIYDVA
                     PGETMFTASDVGWVVGHSYIVYAPLLTGATTILYEGKPVGTPDAGAFWRIISEYGAVT
                     MFTAPTAFRAIKKEDPEATLLAGYDLSHFRQLFLAGERLDPDTYHWATDKLGKPVIDN
                     WWQTETGWPIAANCVGLERLPIKAGSPSVAVPGYDVQILDPTGEQLPAGTEGAIAIKL
                     PLPPGTFPTLWHDDERYVSSYLSAYPGYYLTGDGGFIDEDGYIYVMGRTDDVLNVAGH
                     RLSTGSIEAALAGHPAVAECAVIGVHDDFKGQIPRALVVVKSGVDVEADGDRIRKELV
                     DRVRSEVGAVASLRQVDIVAALPKTRSGKILRKTMREIADGHTPAVPATIEDAGVLDR
                     LAPVLRPHG"
     gene            6290..7210
                     /locus_tag="PLB86_09180"
     CDS             6290..7210
                     /locus_tag="PLB86_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRC65346.1"
                     /translation="MLGALQGFSTIAAVISVGFVLAHVGVLGMEARNILADLAFFVAS
                     PALLLVVLSKAPVSAVLSWGLVVAAGGALLVMAIAAAIAHWRWGMGLGETVMAALCSG
                     YVNSGNLGLPIALYVLGDITYAAPVLLLQLLVLTPVSMTVLDSQLPFAKPGVMSRVAG
                     LLRNPITVGALIGLVLSVTGRQLPSALLDPITLIGNLAVPCMLIAFGVALRLGQRPGK
                     DLGRVATLSGLKLVGQPVIAASLGAALGLRGHELFAVVVMAALPTAQNIFTYSLRYDR
                     GVYLTRDTIFVTTLASAPVILVAALVFGGA"
BASE COUNT         1044 a         2573 c         2462 g         1169 t
ORIGIN      
        1 gatcctccga acgtcccgat gagtgggctc ggcccccgct agattcggta tgccgcccgc
       61 tcacccccgc gggtcgcctc cggtccgcca gggcactcgc gagccggcgt cacggcatac
      121 gggaaggatc cccacatgcg cgccctgacc aaggtgaacg ccggacccgg cttggaactg
      181 gtcgaccgac ccgagccgac ctgcggcccg ctcgatgtca aggtgcgcgt gctgcgcgcc
      241 ggcctgtgcg gcaccgacct gcacctggag cagtgggacg actgggccgc ctcgacggtc
      301 aaggcaccga tggtcatcgg gcacgagttc tacggcgaga tcgtcgaggt cggggaggac
      361 gtgccggcgg ggacggccgt cgccgtcggc gcgcgggtgt cgggcgaggg gcacatcgtc
      421 tgcggtcact gccgcaactg ccgggccggc cgccgccacg tgtgcatcaa caccatcggc
      481 atcggcgtca accgggacgg agcgttcgcc gacttcatcg tcctgcccgc gacaaatgtc
      541 tgggtccagc cggactccat cgaccccgac ctgggcgcga tcttcgaccc gctgggcaac
      601 gcgacccata cggcgctgca atggccgctt gtgggtgagg acgtcctcat caccggggcc
      661 gggccgatcg gggtcatcgc cgcggccatc gcccggcacg cgggagccaa gaacgtcgtc
      721 gtcacggaca tctccgacta tcggctgggg ctggccaaag ccgccggtgc ggatctcacg
      781 gtcaacgtgg ccacgacccg gatcaaggac gcgcagcgcg agttgggcat gcgggaaggc
      841 ttcgacatcg gcctggagat gtccggtgcc ccgtcggccg tggccgagat gctcgccaac
      901 ctcaaccatg gcgccaaggt cgcgatgctc ggcctgccga aggaccccta cccgatcgac
      961 tggggtcggg tgatcaccca catgatcacc ctcaagggca tctacggccg cgagatgttc
     1021 gagacctggt atctcatgtc gtcgatgctc gccacctccg agaccttcac gtcggcggtg
     1081 tcgtcggtca tcacccatcg attccctgcc agccagtggg ccgacgcctt cgcagcggcc
     1141 cgctcaggcg agtgcggcaa ggtcgtcctt aactggagtt aatcctcggc aacctctctg
     1201 gccaatcctg cgttcttgac ccgcgccgac ggaataagct tccggccgtg actccctccg
     1261 acgacctcat ggacgccgct ctggcggtcg tcggagaggc cggagtgccg gggctgacct
     1321 ggaagcggat cgatgagcgg gccggggccg cggagggcgc gaccttctcg ctctacccga
     1381 cgatggacct gctggtcgag gccatgttgg ggcgactgat caccctcgac gggcaggcct
     1441 ggctcgccac cggaaccgcg ctcaacccga cctcggcccg cgagttcgcc gaccggatgg
     1501 ccgccctcac cgtgtcccac atcgagctga tggggcaggc cacccgggcg cggttcgccc
     1561 tgttcctcgg tcggccgcag gcgatggctc agggtcaggc cgccctgctg gacgtcatca
     1621 ccgtcatcct tgactccatc ggcgtccccg accccgccgc gcgagcccaa cacgtcgtcg
     1681 acctcgtcgg tggcaccgcg ttgcacgcct tgaccgttcg tcagggcgag cccatcgatg
     1741 aggccgcgct ggctgccgct ttctggcggt tgctgcgctg accccgacgc gctaactgct
     1801 ctcacccgaa ggagacctcc gtgtacgggt ccatcaagga cgaacttctc gccaccctcg
     1861 ccgagatcga cgctgcgggg ctctacaagc gggagcgtca gctcaccacg gcgcagtcgg
     1921 ctcacgtcgg cgtcggtggt gggtcgccgg ctgtgctcaa cttctgcgcc aacaattacc
     1981 tcggcttggc cgatcacccc gacgtcgtcg ccgcagccaa ggcggccctc gacgagtggg
     2041 gtttcgggat ggcatcggtg cgcttcatct gcggcaccca gacccagcac accctgctcg
     2101 aagatcgcct gtcggccttc ctcggcaccg aggcgacgat cctctactcc tcgtgcttcg
     2161 acgccaacgg tggggtgttc gaggtgctct tcggcgaggg cgacgcgatc atctccgacg
     2221 agctcaacca cgcctcgatc atcgacgggg tgcgcctggc caaggccgcg cgctatcgct
     2281 accgcaacgc cgacatggcc gacctccggg tccagttgga ggccgcgcgt gccgctgggg
     2341 ctcgccgggt cgtcattgtc accgacgggg tcttctcgat ggacggctcc tacgcgccac
     2401 tcccgcagat ctgcgacctc gcggaggagt atgccgcgat ggtcctcgtc gacgactcgc
     2461 acgccgtcgg gttcgtcggg gagggcggcc gggggacgcc ggagctcttc ggcgtcgccg
     2521 atcgggtcga catcgtcacc ggcacgttgg gcaaggccct cggcggtgcc tctggtggct
     2581 atgtctcgag ccaccgggaa gtggtcgacc tgctgcgtca gcgctcgcgt ccctacctgt
     2641 tctccaactc ggtcgctccg gccgttgtcg ctggctcgtt ggccgccctc gacctcgtgg
     2701 catcctccgg tgaggcccga gcggccctgc gcgccaacag cacgctcttc cgggagctga
     2761 tgacggcggc cggcttcgac ctgctgcccg gctcgcaccc catcacgccg gtgatgttcc
     2821 caggcgagga cggggcccgg caggcgacga tgatcgccga ccacatgctc ggcaacggcg
     2881 tctatgtcat tgcgttctcc ttccccgtcg tccccaaggg caaggcacgg atccgggtcc
     2941 aactctcggc cgctcattcg gaggccgacg tgcgcgcctg tgtcgccgcc ttcgtcgctg
     3001 ctcgcgacgc ggtgggctaa aggcgccgcc cgacgctgcg tcagtcagct gaaggaagca
     3061 ggatcgccgc ggcttcctcg atcgtgtcga cgagatgcac gacgtcggcc atcggccgat
     3121 tgcctgccaa ggcggtgacc agcggccagg tcggcacgag acgcccccag tggctacgac
     3181 cgacgagcac cagcggcggc aatgtcccgg tcgggtcggc caaccggccg taatacagcc
     3241 gggtgacccc ttggaagatc tcctgcaccg tcccggcggc ccctggcagg acgactgtcc
     3301 ccgcagtgca tcgagcgagc aacccgtcct cgcgcagggc gttcgagaag tacttcgcga
     3361 tgccctggcc gaacacattg ggcggttcgt ggccgtagaa ccaggtcggg atgccgatgc
     3421 tgcgcaacgg caacccgcgc agttcgccgc tgtcaggccg acgtcccaac tgctctcgga
     3481 cccgcaaccc gagctcggcc cagggcccga cgtcgggccg ataggacggc acctttgaca
     3541 gcgcggcgat ggcggcgtcg aggtccgcgg gggacgcggc atacgccccg aggttggcgg
     3601 cctccatcgc gccggggccg cccccggtgg cgaccacgca gccgccctcg gcgagcaggt
     3661 gccccagccg ggccgccgcc gcgtagacgc cgctgccgcg ttgggcctcg tggccgccca
     3721 tcaccccgac gacggggagc ccgtcgagca cctcgtcgag ggtgtcggtc attgactcgt
     3781 cgtggatcgc ccgcagcatg gtgacgtagg cgtcccggcc cgagcggcga tccatcgtcc
     3841 agtcgaaggc ccgcgcgtcg ggggttccgg cgtagccgag cggaccggac agcccggcat
     3901 acaactcgtc ggcggtgtag aggcgcgcgc ggtagacctc gacgggagcc ttggggtcgc
     3961 tggggaagac gatggcgccg tgcctgatga ggtgcagggt gagcgcgggg gagagccgac
     4021 cgccgagcac gaccaggtcg gtgacatccg tgcggtcgag cagctgctcc tcgacggcgt
     4081 gcagatcgag gtcctgcagc cgcacaccac cgagggcccc gccgcgcgcc agcagcgcgc
     4141 gcaactgctc gaggcggtcg atctccacga tggcactctg ccagagccgc tcgacgggca
     4201 cgaccgttcg gcgcaggtgg gcgcgttccg ggcgcaaagg acggggtagg catccggcgt
     4261 ccggtggcgc gcgggcggca taccgggcac cctcatgacc catgagcact ggcgcttact
     4321 cgaccgcata cgcggcaagc ctggccaacc cgaccgcctt ctggggcgag gcggcgaagg
     4381 acatcgactg gatcacgcca ccgacgacgg tcctggacga cagccggccg ccgttctacc
     4441 ggtggttccc cgacgggacg ctcaacacct gcttcaacgc gatcgaccgg cacgtcgcgg
     4501 gcgggcgcgc ggatcaggtc gccatccact acgactcacc ggtgacgggc cgcaaggact
     4561 ccttcacgta tgccgagttg ctggctcacg tgagtcggct cgccggtgcg ctggtcgggg
     4621 cgggtgtcgg caagggcgac cgggtcctgg tctacatgcc gatggtcccc gaagcggtct
     4681 tcgcgatgct ggcctgtgcg cggatcgggg cgatccactc ggtggtcttc ggcgggttcg
     4741 caccggccga ggtcgccgcc cgcatcgacg acgccaagcc caaactcatc ctctcggcat
     4801 cgtgcggcat cgagcccaac cgggtcgtgc cctacaagcc gttcctcgac gagggcatcg
     4861 cccgatccga gcacaagccc gaccaggtcg tcgtcctgca gcgtgagcag gcgcgggccg
     4921 agttggggga gcgggacgtc gactgggccg agttcatctc gacgccggcc gcggatgctg
     4981 gagccgactg tgtcgaggtc gctgccaccg acccgctcta catcctctac acctcgggaa
     5041 ccaccgggaa gcccaagggc atctaccgcg acaacggcgg gcacgccgtg gccctgcgct
     5101 ggtcgatgcg caacatctac gacgtcgcgc ccggcgagac gatgttcacc gccagcgacg
     5161 tcggctgggt cgtcgggcac tcctacatcg tctacgcgcc actgctcacg ggcgcgacga
     5221 cgatccttta cgagggcaag ccggtcggca cccctgacgc gggcgcgttc tggcggatca
     5281 tctcggagta cggcgcggtg acgatgttca ccgcccccac cgcgttccgg gcgatcaaga
     5341 aggaagaccc cgaggcgacg ctgctcgcgg gctacgacct gtcgcacttc cggcagttgt
     5401 tcctcgcggg ggagcgcctc gaccccgaca cctaccactg ggccaccgac aagctcggca
     5461 agccggtcat cgacaactgg tggcagaccg agaccgggtg gccgatcgcc gccaactgcg
     5521 tggggctcga gcgactgccg atcaaggccg ggtcgccgtc ggtcgccgtg ccgggctatg
     5581 acgtgcagat cctcgacccg accggcgagc agttgccggc gggcaccgag ggcgcgatcg
     5641 cgatcaagct cccgctgccc ccagggacct tcccgacgct gtggcacgac gacgagcgtt
     5701 acgtctcgtc ctacctgtcg gcctaccccg gctactacct caccggtgac ggtggcttca
     5761 tcgacgagga cggctacatc tacgtcatgg gccgcaccga cgatgtcctc aacgtcgctg
     5821 gacaccggtt gtcgaccggc tcgatcgagg ctgccctggc cggccacccg gccgtcgccg
     5881 agtgcgcggt catcggcgtg cacgacgact tcaagggcca gatcccgcgg gccctcgtcg
     5941 tcgtcaagtc tggggtcgac gtcgaggccg acggtgaccg gatccgcaag gagctcgtcg
     6001 accgggtccg ctcagaggtc ggtgcggtcg cgtcgctgcg tcaggtcgac atcgtcgccg
     6061 cccttcccaa gacgcgatca ggcaagatcc tgcgcaagac gatgcgggag atcgccgacg
     6121 gccacacgcc ggccgtgccg gccaccatcg aggacgccgg agttctcgac cggctcgccc
     6181 cggtgctgcg cccgcacgga taggcgcgcg ggcggcatac cggccggtcc gtggggtcaa
     6241 cccacggacg ggtatgccgc cctcccgcgc gcggtgtcac catgcttcgg tgctcggtgc
     6301 gctccagggg ttctcgacga ttgccgcggt catttccgtg ggtttcgtcc ttgcccacgt
     6361 cggggtgctc gggatggaag cccgcaacat cctcgccgac ctggcgttct tcgtcgcctc
     6421 gccggccctg ctgctcgtgg tgctgtccaa ggctccggtg tcggcggtgc tctcgtgggg
     6481 gctggtcgtc gccgcgggcg gggcgctgct cgtcatggcc atcgccgccg cgatcgcgca
     6541 ttggcgctgg gggatggggc tcggcgagac cgtgatggcg gcgctctgct cgggttacgt
     6601 caactcgggg aacctcggcc tgccgatcgc gctctatgtc ctcggcgaca tcacgtatgc
     6661 cgcgcccgtc ctgctcctgc aactgctcgt gttgacgccg gtgagcatga ccgtgttgga
     6721 ctcccaattg ccttttgcca agccgggtgt gatgtcaagg gtggccgggc tgttgcgcaa
     6781 cccgatcacg gtgggggcac tgatcggtct ggtcctgtcg gtgaccggcc gccagcttcc
     6841 gtcggcgctc ctcgacccga tcacgctcat cggcaacctc gcggtgccct gcatgctgat
     6901 cgcgtttgga gttgcgctgc ggttggggca gcggccgggg aaggacctcg gccgggtggc
     6961 aacgttgtcg ggcctcaaac tcgtcgggca gccggtgatc gccgcgtcgt tgggggccgc
     7021 cctcgggttg cgggggcacg agttgttcgc tgtcgtcgtc atggccgcgc tgcccaccgc
     7081 gcagaacatc ttcacctatt cactgcgcta tgaccggggc gtctatctca cccgcgacac
     7141 catcttcgtc acgaccctgg cctcggcgcc ggtgatcctc gtggccgcgc tggtcttcgg
     7201 tggggcctga ccgaccctgg gctgccggtc gcgcccgaat tccccaca
//
LOCUS       DAORPW010000111         7222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2651_length_7222_cov_678.315753, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000111 DAORPW010000000
VERSION     DAORPW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2651_length_7222_cov_678.315753"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2021)
                     /locus_tag="PLB86_09185"
     CDS             complement(<1..2021)
                     /locus_tag="PLB86_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRC65347.1"
                     /translation="MTSQDEMVGATHTALIAVLTLEEKVRLLTGRDTWSTWPIERIGL
                     RSMVFSDGPSGVRGPLWDERSPSLNLPSATALASSWDPEIARRYGNVSAQEARRKGVD
                     VVLGPTINLHRSPLGGRHFEAFSEDPWLTAELAAAYVMGVQENGVGATVKHYVANEYE
                     TERFTASSEVDERTLREVYLRAFEKAVVEARVWVVMSSYNSINGVTASENDLLETPLN
                     SEWGFDGVVISDWTAVRSVAAASASQDLAMPGPDGAWGAALVEAVRDGRVSEAAIDRK
                     VARLLTLAQRVGALESYAGGPADAGGLLRVEDGVAFAREAAAEGTVLLANDGVLPLDV
                     ARLRRVAVIGQNAVAARTQGGGSATVIPERVVSPWEGIVAALPDAEVTYRLGAAAAMG
                     ITALPLAELTNPSTGGPGVLVRFLDDAGVELMREDRFATSLVFFAGNAPTLTAAVLEL
                     SCRWTPTENGDVTVGFASIGRGRISVDGALVLDDVAVGDSTDVGANILDPPKAVAPVA
                     VSVTAGRPVDVVLELDLAIMQETTDGAFGITLGVAPSDTDPEARIADAVAVAAASDVA
                     IVVVGTNAQVESEGFDRSDLRLPGRQDDLVAAVAATGTPTVVVVNSGSPVELPWRDDV
                     SAIVLPYFGGQEMGNALADVLLGRVEPGGRLPTTWAASMADVPVLDVTPV"
     gene            complement(2052..2726)
                     /locus_tag="PLB86_09190"
     CDS             complement(2052..2726)
                     /locus_tag="PLB86_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65348.1"
                     /translation="MPTDVMRAARPLVATGVVYELSDGRRVLDDVALALAPGTMTALT
                     GPSGAGKSTLLWILAGALAPTSGTVMYAAAPVGDRVAAARAGIVLVPQGVALVTALTA
                     RENLVVPLLMHGVGAGEAHARADAALASVLLAESGNHLVEELSGGEQQRVGVARALAL
                     RHSVLLADEPTSELDGATRETIVALLRAEADAGAVVLLATHDSWCADQADAEYHLDEG
                     SLRRVR"
     gene            2815..5928
                     /locus_tag="PLB86_09195"
     CDS             2815..5928
                     /locus_tag="PLB86_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65349.1"
                     /translation="MLGALRYRRAQAVVVVVLSALVTACLVLAPLYTRALEQSSVTSL
                     LRAATPAESGIRLASFSSAEPGIALDPDDLAELIPSSIRARYGTAIAATSVGVRRMPL
                     GPEPGGVLLARDNACAHVTFVEGQCPSAAGEIAVSADQAKVYEHAIGTAVFVGEFDGA
                     VSLPTASPRTTLHVVGIYEQVDGSYWFGERLTGQASARLGFDTMLTPLTTLTERVTAV
                     DGQLTTWFERHYALDLPLLTDTVGVDQIGPLGATVADLVRYPMGVERSGTHVADTVTA
                     RSGLPAIAEQVRVGSAQAGITVPLLMAQASLLLVAVLWLVLVAAADQRRSEVAVARLR
                     GRGSRGARRILLAETLPPIILGAPLGAALAAAAVSAARHTLLPSQPPFEVPVGAVVAL
                     AASLALMLTLAVVSVRRVCREPVAALLRTVPPRRGSVRLGVLEAMLVAAAAAAFVALV
                     TGSVGGAVGQIAPTLLALAVGVIAARVLSAVLAAGGRRLLLAGRSTSGAALLTASRRG
                     TTRWLVPVVTIALCIVVVSADSLAVGARNWSDRAGAEVGAATVLTLDSADLSAVAAAV
                     RAVDPTGEHVTPVALLQPAAEGGTTTVGVVPGAFRRIALWPGIDVAALPWDRLTAPTA
                     PPLVATGSRISYHVTAPALTVVGPAIRPAPTSLALALRVVRADGSVESVPLGTIPATG
                     IDADQDVAVGCSEGCRISGIGVLGPPGAAGVSGTVAVSELAVDGRPFDLGGAGSWHAP
                     APVDPGGTPSGGATVSGSFAPGSTTLTYDTNGADKAFLAHASRPEVVVALTTPTSTPS
                     ATAATFGGSYVDGSSLLLTSAGGVAFVPGGPAAASIVNLDNLLAQGWRGRGSATLTAY
                     LDTADPDAVRRVTTALQANGIHVVATRHAAAVAAAYGETAAAWSLKLALAVGVLAILV
                     AGVGIVVLASTARRARSRDYAALALVGQRPRSLALLAQLETVPVIVASALIGAAVGWW
                     AAPVAVSMMPLFPAASATFPLDLRTAVAPALVAGLGGLVALTLVGVATSHAAARRADL
                     QQLREAG"
     assembly_gap    5950..6049
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6050..6989
                     /locus_tag="PLB86_09200"
     CDS             <6050..6989
                     /locus_tag="PLB86_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC65350.1"
                     /translation="EVTRAQVTRPQVTRAHAITTVGLVHIYRSEGHDVAALSGVDLSV
                     RAGEVVGLLGPSGSGKSTLLALLGGLFRPSAGKIFVGDRELSALTAAELDDFQARDTS
                     LMLQGARRNFVPYLTVRENVEFAQAQARRLGAQVDPVERTLGLVGASDFADARLGRLS
                     QGQAQMAALAVAMAPSPGVLLADEPTSALDRRAREVIIEALLRINAEQGTTVVVVTHD
                     PEVAARLPRTVTIRDGRIGGEGRSGQEYAVVTADGFVPLPVHLREELPPGTLLRIERV
                     EDGYLLAPEVLGHEVLGHEVLGHEVSGHESDGATGA"
BASE COUNT          970 a         2746 c         2349 g         1057 t
ORIGIN      
        1 acgggagtga catcgaggac cggcacatcg gccatgctcg ccgcccaggt cgtgggcaac
       61 cggccacccg gctcaacccg accgagcagc acgtcggcca gcgcattccc catctcctgc
      121 ccaccgaagt agggcagcac gatcgccgaa acgtcgtcac gccagggcaa ttcgaccggt
      181 gacccggagt tgacgaccac cacggtcggg gtgcccgtcg ccgccaccgc cgcgaccagg
      241 tcgtcctgtc gaccgggcag ccgcaggtcg ctgcggtcga agccttcaga ctcgacctgc
      301 gcgttggtcc cgacgacgac gatggccacg tccgaggcgg ctgcgacagc caccgcgtcc
      361 gcgatccgag cctccgggtc ggtgtcggac ggcgccacgc ccagggtgat cccgaacgcg
      421 ccatcggtcg tctcctgcat gatcgccagg tcgagctcaa ggaccacatc gacgggccgc
      481 ccggcagtga ccgacacagc gaccggagcc accgccttcg gcgggtcgag gatgttggct
      541 cccacgtcgg tgctgtcgcc gaccgccaca tcgtccagca ccagcgcccc gtccacgctg
      601 atccgcccgc ggccgatcga cgcgaatccc actgtcacat caccgttttc ggtcggcgtc
      661 caccggcacg acaactccag cacagccgcc gtcagggtcg gagcgttgcc tgcgaagaac
      721 accaacgagg tcgcgaaccg gtcctctcgc atcaactcga caccagcatc gtcgaggaag
      781 cgcaccaaca caccaggccc accggtcgac ggattggtca actccgccaa cggcagcgcc
      841 gtgatcccca tcgccgccgc cgcccccagc cgataggtca cctcggcgtc cggcagcgcc
      901 gcgacaatcc cctcccacgg gctgacgacg cgctccggga tgaccgtcgc cgacccgccg
      961 ccctgggtcc gcgcggccac cgcgttctga ccgatcaccg cgacccgacg cagccgcgcc
     1021 acatccaacg ggagcacccc atcgttggcc aacaacaccg tgccctcagc cgccgcctcc
     1081 cgcgcgaagg caacgccgtc ctcgacccgc agcaagcctc cagcatcagc cggcccaccg
     1141 gcatacgact caagcgcccc gacccgctgc gcgagcgtca acaaccgcgc gaccttccgg
     1201 tcgatcgccg cctccgacac ccgcccgtcc cggaccgcct cgaccaacgc ggcaccccac
     1261 gccccgtccg gccccggcat cgccaggtcc tgcgacgccg acgcggccgc aacgctgcgg
     1321 accgcggtcc agtcgctgat caccaccccg tcgaagcccc actcggagtt cagcggcgtc
     1381 tccaacaggt cgttctccga cgcggtcacc ccgttgatcg agttgtagga cgacatcacc
     1441 acccacaccc gcgcctcgac gaccgccttc tcgaacgccc gcagatagac ctcgcgcaac
     1501 gtccgctcgt ccacctcgct gctcgcggtg aacctctccg tttcatactc gttggccacg
     1561 tagtgcttca ccgtggcccc aacgccgttc tcctgcaccc ccatcacgta tgccgcggcc
     1621 aactccgcgg tcagccacgg gtcctcgctg aacgcctcga aatgccggcc acccagcggc
     1681 gaccgatgca ggttgatcgt cgggcccagc acgacatcga cgcccttgcg ccgggcctcc
     1741 tgcgccgaga cgttgccata acgccgggcg atctccgggt cccacgacga cgccagcgca
     1801 gtggccgacg gcaggttcaa cgacggcgag cgctcatccc acagcggtcc gcgcactccc
     1861 gacggcccat ccgaaaacac catggaccgc aacccgattc gctcgatcgg ccacgtcgac
     1921 caggtgtccc ggccggtgag cagcctgacc ttctcctcca gggtgagcac cgcgatgagc
     1981 gcggtgtgag tcgccccaac catctcgtcc tgcgatgtca tccccgcgcc tctccctgac
     2041 tggtcaactg gtcatcgaac ccgccgcaac gatccctcgt cgaggtgata ctccgcgtcc
     2101 gcctgatcgg cgcaccacga gtcatgcgtc gccagcagca cgaccgcccc cgcgtcagcc
     2161 tcggcgcgca acaacgccac gatcgtctcg cgggtcgccc cgtccaactc gctcgtcggc
     2221 tcgtccgcga gcagcacgct gtgtcgcaac gccaaggccc gcgcgacccc gacccgctgc
     2281 tgctcgccgc cggacaactc ctcaaccagg tggttgcccg actcggccag cagcaccgac
     2341 gcgagcgccg cgtccgcccg cgcgtgcgcc tcccccgcgc cgacgccgtg catcagcagc
     2401 ggcacgacga ggttctcccg cgccgtcagg gccgtcacga gggcgacccc ctgcggcacg
     2461 aggacgatcc ccgcacgcgc ggcagcgacc cgatcgccta ctggcgcagc ggcatacatc
     2521 accgtccctg acgtcggagc gagcgcgccg gcgaggatcc acagcaaggt cgacttgcct
     2581 gcccccgacg gtccggtgag cgcggtcatc gtccccggcg cgagcgcgag cgcgacgtcg
     2641 tccaggaccc gccgcccgtc actcaactcg tagacgacgc ccgtcgccac cagcggccgg
     2701 gcggcccgca tcacgtcggt cggcacggct cccgctcctc ctgcctgcca gtgggtatgc
     2761 cgtgtacggt acaggcgcgg gcgccgcacc gaccgtgccg cacggggagg gcagatgttg
     2821 ggagcgttgc gctaccgacg agcacaggct gtcgtcgtcg tcgtgctctc ggccctcgtc
     2881 acggcgtgcc tcgtgctcgc ccccctttac acccgggcgc ttgagcagtc gtccgtcacc
     2941 agcctcctgc gcgccgccac tccggcagag tccggcatcc gcttggcctc gttcagctcg
     3001 gccgagcccg gcatcgcgct cgaccccgac gacctggccg agctcatccc gagcagcatc
     3061 cgcgcccgct acgggacggc gatcgcagcg acgtcggtgg gcgtccggag gatgccgctg
     3121 gggccagagc ccggcggggt gctcctcgcc cgggacaacg cctgcgccca cgtcaccttc
     3181 gtcgagggtc agtgcccgag cgccgccggc gagatcgccg tgtccgccga ccaggccaag
     3241 gtctatgaac acgccatcgg gacagccgtg ttcgtcggcg aattcgacgg cgccgtcagc
     3301 ctgcccaccg cctccccccg caccaccctc cacgtcgtcg gcatctacga acaggtcgac
     3361 gggagctact ggttcggcga gcgcctgacc gggcaggcct ctgccaggct cggattcgac
     3421 acgatgctca ccccgctgac cacgctcacc gagcgggtga ctgccgtcga cggccagttg
     3481 accacgtggt tcgagcggca ctacgccctc gatctgccgc tgctcaccga caccgtggga
     3541 gtcgatcaga tcggcccgct cggagcgacc gtcgccgacc tcgtccgcta cccgatgggc
     3601 gtcgaacggt ccggcaccca tgtcgccgac acggtcaccg cgcgcagcgg cctgcccgcg
     3661 atcgccgagc aggtccgcgt cggcagcgcc caggccggca tcacggtgcc gctgctcatg
     3721 gcccaggcga gcctgctgct cgtcgctgtc ctctggctgg tcctcgtcgc cgccgccgac
     3781 cagcgccgca gcgaggtcgc cgtggcccgg ctgcgcggcc gcggctcgcg tggcgcccgc
     3841 cggatcctgc tcgccgagac cctcccaccg atcatcctcg gcgctcccct gggagcagcc
     3901 ctggcggcgg ccgcggtgtc ggcggcacgg cataccttgc tgccgtccca gccgcccttc
     3961 gaggtgccgg tcggagcggt cgtcgcgctc gccgcgtcgc tcgcgctcat gctcactctc
     4021 gcggtcgtct cggtccggag ggtatgccgc gagcccgtcg ccgccttgct gcgcaccgtc
     4081 ccgccgcggc gaggaagcgt gcggctgggc gtcctcgaag cgatgctcgt cgcggcggct
     4141 gcggcggcgt tcgtcgcctt ggtcaccgga tcggtcggcg gcgcggtcgg gcagatcgcg
     4201 ccgacccttc tcgcgctcgc tgtcggggtg atcgcggcgc gcgtcctgtc ggccgtcctc
     4261 gccgccgggg gccggcgact cctgctcgcc ggacggtcga cgtcaggtgc tgccttgctg
     4321 accgccagcc gccgcgggac cacccggtgg ctcgtccctg tcgtcaccat tgcgctgtgc
     4381 atcgttgtcg tcagcgcgga ttcgctggcc gtcggggctc gcaactggag cgatcgggcc
     4441 ggcgcagagg tgggtgccgc caccgtgctg accctcgact ccgccgacct gtccgccgtc
     4501 gccgctgcag tgcgggccgt cgacccgacg ggcgagcacg tcacgccggt tgccctcctg
     4561 cagccggccg cggagggcgg cacgaccacc gtcggcgttg tcccgggggc gttccgtcgg
     4621 atcgccctgt ggcccggaat cgacgtcgcc gccctgccgt gggaccggct cacggccccg
     4681 accgctcccc cgttggttgc caccgggagc cggatctcct atcacgtcac cgctccggcc
     4741 ttgacggtcg tcgggcccgc gatccggccc gccccgacct cgttggccct ggccctgcgc
     4801 gtcgtccgag ccgacgggtc ggtcgagtcg gtccccctcg ggacgatccc cgcgactggc
     4861 attgatgccg atcaggatgt tgccgtcggc tgctcggagg gctgtcggat cagcgggatc
     4921 ggggtcctgg gcccacctgg agcggcgggc gtttccggca ccgtggcggt gagcgagttg
     4981 gccgtcgacg ggcgcccgtt cgacctcggc ggcgcaggct cgtggcacgc accggcgccc
     5041 gtagacccgg gcggaactcc ctcgggcgga gccacggtca gcgggagttt cgcgcccgga
     5101 tcgaccacgc tgacctatga caccaacggc gccgacaagg cgttcctcgc tcacgcctcg
     5161 cggcccgagg tcgtggtcgc gctgaccacc ccgacctcca caccgagcgc cactgccgcc
     5221 accttcggtg ggtcctacgt cgacggaagc tcgctgttgc tcacgtccgc tggcggggtg
     5281 gcattcgtcc ccggcggccc cgccgcagcg agcatcgtca acctcgacaa cctgctggcc
     5341 cagggatggc gcgggcgcgg gtcggccacg ctcacggcat acctcgacac ggccgacccc
     5401 gacgctgtca ggcgagtgac aacggccttg caggccaacg ggattcacgt ggtggcgacg
     5461 cggcatgcgg cggcggtggc cgcggcatac ggggagaccg cagccgcgtg gagtctcaaa
     5521 ctggcccttg cggtgggtgt cctggcgatc cttgtcgccg gggtggggat cgtcgtcctc
     5581 gcctcgaccg cccgtcgcgc ccgcagccgt gactatgccg cgctggccct cgtcgggcag
     5641 cgtccccgca gcctcgcgct gctggcccag ctcgagaccg tgcccgtcat cgtcgcgtca
     5701 gcgctgatcg gcgccgccgt cgggtggtgg gcggccccgg tggccgtgtc gatgatgccg
     5761 ctcttcccgg ccgcgtcggc gaccttcccc ctcgacctgc gcacggcggt ggcgcccgcg
     5821 ctcgtcgccg gtctcggcgg cctggtcgcc ctcactctcg ttggcgtcgc cacaagccac
     5881 gcggccgcgc gccgggccga tctgcagcag ttgcgggagg ccggatgact cgcgccgagg
     5941 tcacccgcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gaggtcaccc
     6061 gcgcccaggt cacccgcccc caggtcaccc gcgcccacgc gatcacgact gtggggctgg
     6121 tccacatcta ccggtccgag gggcacgatg tcgctgccct gtccggggtc gacctcagtg
     6181 tccgcgcggg cgaggtggtc gggctgctcg gtccgtcggg gtcggggaag tcgacgttgc
     6241 ttgcgctgct cggcggtctg ttccgaccga gcgcgggcaa gatcttcgtt ggtgaccgcg
     6301 agctatccgc gctcaccgca gccgaattgg atgacttcca ggcgagggat acgtcgctca
     6361 tgctccaagg ggcgcgacgc aacttcgtgc cctatctgac ggtgcgcgag aacgtcgagt
     6421 tcgcgcaggc gcaggcccgc cggctcggcg cgcaggtcga cccggtcgag cggaccctgg
     6481 ggctggtcgg ggccagcgac ttcgccgacg cgcgcctggg tcgcttgtcg cagggacagg
     6541 cgcagatggc ggcgcttgcc gtggcgatgg caccgtcgcc tggggtgctg ttggccgacg
     6601 agccgaccag tgcactggac cggcgcgctc gcgaggtcat catcgaggcg ttattgcgga
     6661 tcaacgcgga gcagggcacg actgtcgttg tcgtcaccca cgaccccgag gtcgccgccc
     6721 gcctcccgcg cacggtgacg atccgcgacg gccggatcgg gggtgagggc cgcagcggcc
     6781 aggagtatgc cgtcgtcacg gccgacgggt tcgtccccct gccggtgcac ctgcgggagg
     6841 agttgccgcc gggcacgctg ttgcgcatcg agcgggtcga ggacggctat ctgttagctc
     6901 ccgaggtctt gggtcatgag gtcttgggtc atgaggtttt gggtcatgag gtttcgggtc
     6961 acgagtcgga cggtgcgaca ggcgcttagg gcgtgccgac gcggtcggcc gcgccgttgg
     7021 gcgccggtga ggtgggacga ctcgcccgac tgaccagccg actgcggcag acggcgacgg
     7081 ctgacgacga cagccgaccg tcagatcgtt atcattctgt gatgtgtttg cctgtggaag
     7141 aggctgtttg agacctattg cctgtcagtg gtcgctgtta gcgtcggggt tatggacagc
     7201 agcgaagcga ctcgtgactc gg
//
LOCUS       DAORPW010000112         7179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2676_length_7179_cov_735.279057, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000112 DAORPW010000000
VERSION     DAORPW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2676_length_7179_cov_735.279057"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLB86_09205"
     CDS             complement(<1..605)
                     /locus_tag="PLB86_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HRC65351.1"
                     /translation="MTGRGTTGPILNGIPSQLPDTDPEETQEWLDSLQGVIDEQGRTR
                     ARYLMLQLLAAARGQQVGVPSLTATDYINTIPPEREPWFPGDEDIERAYRRWIRWNAA
                     VMVHRAQRPGVGVGGHISTYASAATLYEVGMNHFFRGQDHPGGGDQVFFQGHAAPGMY
                     SRAFLEGVLEEEDLDGFRQERSHVVDGKIRALSSYPHPRLMP"
     gene            820..1248
                     /locus_tag="PLB86_09210"
     CDS             820..1248
                     /locus_tag="PLB86_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HRC65352.1"
                     /translation="MHGSAGVAAFTSGLSKLGFTPGQVVQELGWDDDVDEALRAGVEQ
                     VVGSALEDESYADGADGVLVWFRSYDGDLVDVLVDALTNLVDRGCLVLATPRADQDGH
                     VEPSEIEDAAVAAGLHPSGVANLSREWTVQRLVAPRSQRR"
     gene            1297..1806
                     /locus_tag="PLB86_09215"
     CDS             1297..1806
                     /locus_tag="PLB86_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRC65353.1"
                     /translation="MSAEPDAVAPAAPEVGDVPPDFTLPDQYGRQVSAATWRGDRNVV
                     LVFYPFAFTGICTGELREIRDTLEDFQHDGIQVLAISCDPVPALRAWADQEGYFFPLL
                     SDFWPHGEVSRAFGVFDADGGFATRGTFLLDRDGVVRWRMLNGPGDARDFGSYREALA
                     GLRADAARA"
     gene            1849..1921
                     /locus_tag="PLB86_09220"
     tRNA            1849..1921
                     /locus_tag="PLB86_09220"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1882..1884,aa:Val,seq:tac)
     gene            1929..2765
                     /locus_tag="PLB86_09225"
     CDS             1929..2765
                     /locus_tag="PLB86_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRC65354.1"
                     /translation="MTLGQIVALLDGFYPPATALSWDRVGLVTGDPDQPVGHVRFAVD
                     PTLAVIEEAIADGADLLVTHHPLLLRGIHSVATTTAKGRAVTRAVVADLAILCAHTNA
                     DVADPGVCDALAAACGLVDTEPLDIEQGQGVGRVGRLAQPTSLRAFAEGLAAALPPTN
                     VGVRVSGDPDAQVCRVAVLGGSGDDRFGAARRSGVDVYVTADLRHHPALEAREESSFG
                     PPYLIDAGHYATEWLWLTGAAARLSQALADQGTTVETSISTLRTDPWDFLVGVNADGG
                     TP"
     gene            2762..3502
                     /locus_tag="PLB86_09230"
     CDS             2762..3502
                     /locus_tag="PLB86_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRC65355.1"
                     /translation="MKAQPHQQARLIEVQLLDTRLDQLAHARAHLPALTKLAGLDQAQ
                     VTLDRELALARTALSDVQREVDKAEADVQLVRARAARDQARLDSGTGTPKDLQALQSE
                     LVSLARRQGDLEEIELEVMERAESLEADVTSLETRRSALLEELAQVRAERDAAFEVSD
                     AEAADVTERRSALVPEVGAELLALYDKIRASVGGTGASELRQRRCGGCRLELNQVDLS
                     RIKAAPADEVIRCEECRRILVRTAESGL"
     gene            3505..4662
                     /locus_tag="PLB86_09235"
     CDS             3505..4662
                     /locus_tag="PLB86_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional RNase H/acid phosphatase"
                     /protein_id="HRC65356.1"
                     /translation="MARRFIVEADGGSRGNPGVAGFGALVRDADTGAVLLERAEPLGL
                     ASNNVAEYAGLLAGLEGALRLDPAPDIEVRMDSKLVVEQMSGRWKIKHEDMRRLAGQC
                     QDVVRRILAGGGRVRYTWIPREKNKAADALSNHAMDGKTIDREPGKSQPPATAAAVPA
                     STAAVRASAAAVPAPAATLVGEARVILVRHGVTDFTVSGRLDGRGGADPELNAEGLAQ
                     AAAVARIIPGLVGDQQVRLVTSSLRRTQMTGAAIAAALGVVPEADPDWDERAFGEWDG
                     LTSAQIQERSPMKLARMRVDAAYPPPGGESRSEVAARVGRAFERATAAAGTVVVVTSR
                     VPILVVLGQVLQVGAERFWALETEPASVSIVTLWPDGNVSVPLVNRTDHLH"
     gene            complement(4692..5441)
                     /gene="yaaA"
                     /locus_tag="PLB86_09240"
     CDS             complement(4692..5441)
                     /gene="yaaA"
                     /locus_tag="PLB86_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HRC65357.1"
                     /translation="MIVLLPPSETKHQPSRGKALDLAGLSFPALGPTRAAVLAELADL
                     SGQPSALEVLGVSAGLADQVARNQRLATAPTATAGAIYTGVLYDALDLASMDSSARRR
                     AARRLVVVSALFGALRITDRIPAYRLSMGVTLPAQGRLAALWREPLAQVLPDEVGRGL
                     IVDARSAAYAAAWTPTGALAERWVHIRVPGVSHLAKHTRGLVARAACESASDPRHPAG
                     LATILTDAGFRVELGAPAKRGAPWILDVTAP"
     gene            5481..6254
                     /gene="hisF"
                     /locus_tag="PLB86_09245"
     CDS             5481..6254
                     /gene="hisF"
                     /locus_tag="PLB86_09245"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRC65358.1"
                     /translation="MTVATRVIACLDVDAGRVVKGVNFANLRDAGDPVELARSYDAQG
                     IDELTFLDVTASSGDRETTYDVVRRTAEQVFIPLTVGGGVRAVDDVDKLLRAGADKVG
                     VNTAALARPELISEIADRFGAQVLVLSADVRRRRGPDGSLVGGFVMTTHGGRVSVEQD
                     AVAWCARAAELGAGEILLNSMDADGTKDGFDLDLIAAVRAEVAIPVIASGGAGKVEHF
                     APAVRAGADAVLAASVFHFGELTVAQVKAALRAEGFEVR"
     gene            6251..6889
                     /locus_tag="PLB86_09250"
     CDS             6251..6889
                     /locus_tag="PLB86_09250"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC65359.1"
                     /translation="MSTDPAREPTLWEQKLAVDPGHSSWYVKRFRRLASDGVDIVGEA
                     RTVDAMLDRRSRVLDAGCGPGRVGGHLHAAGHTVVGVDIDPVLIEAAEHDHPGPTWLV
                     GDLAVLDLAARGIPEPFDVVVSAGNVLPFAAPSTRADILRRFWEHLVPGGRAIIGFGT
                     DRGYEADTFREHAAAAGFHEDLLLSTWDLRPYAASSDFLVAVLRRGSGVAPA"
     gene            6905..>7179
                     /locus_tag="PLB86_09255"
     CDS             6905..>7179
                     /locus_tag="PLB86_09255"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRC65360.1"
                     /translation="MSVALGMPSIPSQPVIASRRASRKIQVGKVAVGGDAPISVQSMC
                     TTLTADVNATLQQIAELTASGCDIVRVACPSQDDADALGDIARKSQIP"
BASE COUNT         1011 a         2506 c         2577 g         1085 t
ORIGIN      
        1 ggcatgagcc gcgggtgcgg gtaggacgac agcgcccgga tcttgccgtc gacgacgtgg
       61 gagcgctcct gacggaaacc gtcgaggtct tcctcttcaa gcacgccctc gaggaaggcc
      121 cgcgagtaca tccccggagc cgcatgcccc tggaagaaga cctggtcacc gccacccggg
      181 tggtcctggc cgcggaagaa gtggttcatc ccgacctcgt agagcgtcgc agccgacgca
      241 taagtcgaga tgtgcccgcc gacgcccacg cccgggcgct gagcccggtg gaccatcacg
      301 gccgcgttcc agcggatcca ccggcggtag gcccgctcga tgtcctcgtc accggggaac
      361 cagggctcac gctccggcgg gatggtgttg atgtagtcgg tcgcggtcag cgacggcacc
      421 ccgacctgtt gaccacgcgc ggcagccagc aactgcaaca tgagatagcg cgcccgggtc
      481 cggccctgct catcgatcac tccttggagc gagtcgagcc attcctgcgt ctcttccgga
      541 tcggtgtccg ggagttggct cggaatgccg ttgaggatcg gcccggtggt gccacgacct
      601 gtcacggggg acttcctttc tgagcaacat ggatgcgccc atcctgtacc ccgatgcgcg
      661 ctgcgtcaca ttcgtcccgc cagtcgatcc gacgcggacc cgatttcggt gacgatgcgg
      721 catacgtgag cgtcaacaca gcccgtcggc gtcccgcctc gggcagactg ggaccgagcg
      781 agcggctagt ggagggtcgc cacggcagga ggggtcggcg tgcacgggtc ggcgggtgtc
      841 gccgcattta catcgggtct cagcaaactc gggttcaccc ccgggcaggt cgtccaggag
      901 ttgggttggg acgacgacgt cgacgaggcg ttgcgcgccg gagtcgagca ggtggtcggc
      961 tcggcccttg aggacgagag ctacgccgac ggcgccgacg gggtgctcgt ctggttccgc
     1021 tcctatgacg gagacctcgt cgatgtcctc gtcgatgcgc tgaccaacct cgtggaccgc
     1081 ggctgcctcg tcctggccac gccccgagcc gatcaggacg ggcacgtcga gcccagcgag
     1141 atcgaagacg cggccgtggc tgccggactg cacccgtcgg gtgtcgccaa cctcagccgg
     1201 gagtggaccg tgcagcgact ggtggcccct cgatcgcagc gtcgctgacc ctcacgtcga
     1261 ccggtcgggc tcgctcgggt ggcagaatcg atcgtcatga gcgctgaacc cgacgccgtg
     1321 gcgcccgccg cgcccgaggt cggggacgtt cccccggact tcacccttcc cgaccagtac
     1381 ggccgccagg tcagcgcggc cacctggcgc ggggaccgca acgtcgtcct ggtcttctac
     1441 ccgttcgcct tcacgggcat ctgcaccggg gagttgcgcg agatccgcga caccctcgaa
     1501 gacttccagc acgacggcat acaggtgctc gcgatctcct gcgacccggt gcccgcgttg
     1561 cgcgcttggg ccgaccagga ggggtatttc ttccctctcc tgtcggactt ctggccacac
     1621 ggcgaggtct cccgggcctt cggggtcttc gacgccgacg gcggcttcgc cacgcgcggc
     1681 accttcctgc tcgaccggga cggcgtggtc cgttggcgga tgctcaacgg gccgggcgac
     1741 gctcgcgact ttggtagcta ccgggaagcc ctcgcgggcc tgcgcgcaga cgccgcgcgc
     1801 gcctaactga cacaatgggc gccgctcgcc cccgtgggcg gcgagttggg gcctgtagct
     1861 cagttggtag agcaccgcgc ttacaccgcg ggtgtcgtcg gttcgagccc ggccgggccc
     1921 acatgacggt gacgcttgga cagatcgtcg cgctgctcga cggcttctat ccgccggcga
     1981 cggcgttgtc gtgggatcgg gtcggcctgg tgacggggga ccccgaccag ccggtcggcc
     2041 acgtgcggtt cgctgtcgac cccaccttgg cggtcatcga ggaggccatc gccgacgggg
     2101 cggacctatt ggtgacccac cacccgctgc tgctgcgcgg catacattcc gtcgcgacga
     2161 ccacagcgaa gggccgcgcg gtgacccgag ccgtggtcgc cgacctcgcg atcctctgcg
     2221 cccacaccaa cgccgacgtc gcggacccgg gcgtgtgcga cgcgctcgcc gccgcctgcg
     2281 ggctggtgga caccgagccg ctcgacatcg agcagggcca aggggtcggt cgggtgggtc
     2341 gcctcgcgca gcccacgtcg ctgcgtgcct tcgctgaagg gctcgctgcc gcccttccgc
     2401 cgaccaacgt cggcgtccgc gtgtctggag accccgacgc ccaggtatgc cgtgtcgccg
     2461 tcctcggcgg ttccggcgac gaccgcttcg gcgcggcccg gcgaagcggc gtcgatgtct
     2521 acgtcaccgc cgatctgcgt caccacccgg cgctggaggc tcgtgaggaa tcgtccttcg
     2581 gcccgccgta tctcatcgat gcgggtcact acgccaccga gtggctctgg ctgaccgggg
     2641 ccgccgcccg cctgtcgcaa gcgctggctg accagggcac tacggtggag acgtcgatct
     2701 cgaccttgcg caccgacccg tgggacttcc tcgtcggtgt gaacgccgat ggaggtaccc
     2761 cctgaaagcg caaccgcacc agcaggcccg gctgatcgag gtccaactgc tcgacacccg
     2821 gctcgaccaa ttggcccatg cccgcgctca cctgccggcc ttgaccaaac tggccggcct
     2881 ggaccaggcc caggtcactc tcgatcgcga gctcgcgctg gcccggacgg cgctgtcgga
     2941 cgtgcagcgc gaggtcgaca aggccgaggc cgatgtccaa ctcgtgcgtg cgcgggccgc
     3001 ccgcgatcag gctcgtctcg actcgggcac cgggaccccc aaggatctgc aggccctgca
     3061 gagcgagctc gtttcgctgg cccgccgcca aggcgacctc gaggagatcg agttggaggt
     3121 catggagcgg gccgagtcgc tcgaggccga cgtcacgtcg ctcgagaccc gtcgcagcgc
     3181 gttgctggag gagttggcac aggtgcgcgc cgaacgcgac gccgccttcg aggtgtccga
     3241 cgccgaggcc gctgacgtca ccgaacgccg gtcagccctg gtgccggagg tgggcgcgga
     3301 gttgctcgcc ctctacgaca agattcgtgc gagtgtcggc gggaccggtg cctccgagtt
     3361 gcgccagcgc cgttgcggcg gctgccgttt ggagctcaac caggtcgacc tgtcccgcat
     3421 caaggccgcg ccggccgacg aggtcatccg ctgcgaggaa tgccgtcgca tcctcgtgcg
     3481 caccgccgag tccggactgt aggcgtggct agacggttca tcgtcgaggc ggacggcggc
     3541 tcacgcggca accccggggt ggccggtttc ggcgcgctgg tgcgcgacgc ggacaccgga
     3601 gcggtccttc tggagcgcgc cgaacccctg gggttggcca gcaacaacgt cgctgagtat
     3661 gccggtctgc tcgccggtct cgaaggtgcc cttcgcctcg acccggcgcc cgatatcgag
     3721 gtccggatgg actcgaagct cgtcgtcgag cagatgtcgg ggcgttggaa gatcaagcac
     3781 gaggacatgc gccgcctcgc cgggcagtgt caggacgtcg tgcgtcggat cctcgccgga
     3841 ggcggccggg ttcgctacac gtggatcccg cgggagaaga acaaggcagc cgacgcgctg
     3901 tccaaccacg cgatggacgg caagaccatc gaccgcgagc cggggaagtc acagcccccg
     3961 gcgaccgcag cagctgtccc ggcgtccacg gcagcagtcc gggcgtctgc agcagcggtc
     4021 ccggcgccag cggccaccct cgtcggtgag gcccgggtga tcctcgtgcg gcacggcgtc
     4081 accgacttca ccgtgtcggg gcgcctggac gggcggggcg gcgctgaccc ggaactcaac
     4141 gccgaaggtc tggctcaggc ggccgcggtc gcgcggatca tccccgggct ggtgggtgac
     4201 cagcaggtgc ggctggtcac gtcatcgttg cgccggaccc agatgaccgg ggcggcgatc
     4261 gctgcagcgt tgggtgtggt tcccgaggcg gatcccgact gggacgagcg cgcattcggt
     4321 gaatgggacg ggctcacctc ggcgcagatc caggagcgca gcccgatgaa gttggctcgg
     4381 atgcgggtcg acgcggcata ccctccgccg ggcggtgagt cgcgcagtga ggtcgccgcg
     4441 cgcgtgggac gagccttcga acgggcgacc gccgcggcgg gcaccgtggt cgtcgtcacg
     4501 agccgcgtgc cgatcctcgt cgtgctcggg caggtgttgc aggtgggtgc ggagcggttc
     4561 tgggccctgg agaccgagcc ggcgtcggtg agcatcgtca ccctgtggcc cgacggcaac
     4621 gtgtcggtgc cattggtcaa ccggaccgac cacctgcact gatcgctggt gccgacggcc
     4681 tgtgaccgtg gctaaggcgc ggtgacgtcc aagatccagg gtgcgccgcg tttagccggt
     4741 gcccccaact cgacccggaa gcccgcatcg gtgaggatcg tggccagccc cgccggatga
     4801 cgtgggtcgg acgcgctctc gcaggcggcg cgggcaacca gcccccgagt gtgcttggcc
     4861 aggtgactca cgcccggcac gcggatgtgg acccatcgct cggccagcgc gcccgtgggg
     4921 gtccaggcgg cggcatacgc ggccgatcga gcgtcgacga tcagcccgcg gccgacctcg
     4981 tcgggaagga cctgcgccag cggctcgcgc cacaacgcgg ccagtcgccc ctgggccggg
     5041 agcgtgacgc ccatcgacag gcgataggcg gggatccggt cggtgatccg cagcgccccg
     5101 aagagggccg agacgacgac caggcgccgg gcggcgcggc ggcgcgcgga ggagtccatg
     5161 ctggccaggt cgagggcgtc gtagaggacg ccggtgtaga tcgctcccgc cgtcgcggtc
     5221 ggtgccgtcg ccaggcgctg gttgcgggcc acctggtcgg cgagtccggc cgagacgccg
     5281 agcacctcga gggccgacgg ctgcccactg aggtcggcca gttcggcgag cacggccgcg
     5341 cgagtggggc cgagggcggg gaaggacagc ccggcgaggt ccagggcctt gccgcgcgag
     5401 ggctggtgct tggtctccga cggcggcagc aggacgatca ccgcgccagc ctagggttcg
     5461 ggggcaacta gatttggcgg gtgaccgttg ccacgagagt gatcgcctgt ctcgacgtcg
     5521 acgccggccg ggtggtcaag ggcgtcaact tcgccaacct gcgcgacgcc ggggacccgg
     5581 tcgagctcgc ccgcagctat gacgcccagg ggatcgacga gctgaccttc ctcgatgtca
     5641 cggccagcag cggcgaccgg gagacgacct acgacgtcgt ccggcgcacc gccgagcagg
     5701 tcttcatccc gttgacggtc ggcgggggag tgcgggccgt cgacgacgtc gacaaactgc
     5761 tgcgcgccgg ggccgacaag gtcggcgtca acaccgcggc cctggcccga cctgagctga
     5821 tctccgagat cgccgatcgg ttcggcgcgc aggtgctcgt cctgtccgcc gacgtgcgcc
     5881 gccgccgagg ccccgacggg tccctggtcg gcggcttcgt catgacgacc cacggtggcc
     5941 gggtctcggt cgagcaggat gctgtcgcct ggtgcgcccg cgcggcggaa ctcggggcag
     6001 gcgagatcct gctcaactcg atggacgccg acggcacgaa ggacggtttc gacctcgacc
     6061 tgatcgctgc ggtgcgcgct gaggtggcga tccccgtgat cgcgtcgggt ggggcgggca
     6121 aggtcgagca cttcgcccct gcggtgcgag ccggcgccga cgccgtgctg gccgcgagcg
     6181 tcttccactt cggtgagctc accgtggccc aggtcaaggc ggccctacgc gccgaggggt
     6241 tcgaggtgcg atgagcaccg acccggcccg cgaaccgacc ctctgggagc agaaactcgc
     6301 cgtcgatccg ggccactcgt cgtggtacgt caagcggttc cgacgtctgg cctccgacgg
     6361 cgtcgacatc gtcggtgagg cccgcaccgt cgacgcgatg ctcgaccggc gctcgcgggt
     6421 gctcgatgcc gggtgcggtc ccggtcgggt tggcggccac ctccacgcgg ctggccacac
     6481 tgtcgtcggc gtcgacatcg acccggtgct catcgaggcg gccgagcacg accaccctgg
     6541 accgacctgg ctcgtcgggg acctggcggt cctcgatctg gccgcgcgcg gcatacctga
     6601 gccgtttgac gtcgtcgtgt ccgcggggaa cgtcctaccc ttcgcggccc cgtcgacccg
     6661 cgccgacatc ctgcgccgct tctgggagca cctcgtcccg ggcggccgcg cgatcatcgg
     6721 cttcggcacc gaccgcggct acgaggccga cacgttccgt gagcacgccg cggccgccgg
     6781 gttccacgag gacctgctgc tgtcgacgtg ggacctgcgc ccgtatgccg cctcgtccga
     6841 cttcctcgtc gccgtcctgc gtcgcggctc gggggtcgcc cccgcgtaga ggacaatggt
     6901 gggcatgtcc gtcgctctcg gtatgccgtc catcccctcg cagcctgtca tcgcttcgcg
     6961 gcgagcgtcg cgcaagatcc aggtgggcaa ggtggccgtc ggcggtgacg ccccgatctc
     7021 ggtgcagtcg atgtgcacga ccctgaccgc cgacgtcaac gcgacgctgc agcagatcgc
     7081 ggagttgacc gcgtccgggt gcgacatcgt ccgggtcgcg tgccccagcc aggatgacgc
     7141 cgacgcgctc ggggacatcg cccgcaagag ccagatccc
//
LOCUS       DAORPW010000113         7155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2686_length_7155_cov_848.972958, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000113 DAORPW010000000
VERSION     DAORPW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2686_length_7155_cov_848.972958"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PLB86_09260"
     CDS             <1..980
                     /locus_tag="PLB86_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRC65361.1"
                     /translation="TDVAEALRIQRELLGDHDEQADGVRGIPSLPELPARGPGGDIIG
                     RTAGLLVDLAVDLQPAGWRFVDRPGRDAARTAAFWGEDLDELAAAFDGYAGPLKVAVA
                     GPFTLAADIWLHRGERAIVDPGATRDLSESLAEGLRTLLVRLTRLVPGAELIVQVDEP
                     SLPAVLDGRLPTASGFGRLRAIDVQDALPALQTVLAAAGEHGTIVHCCAAYPPLPLLR
                     RSGAGGLSVDTTLITPRGWEGLAVAVEDGVRLHAGCVPTAPEAVVDPVAVADHLASAW
                     SRVGMPPAHLDDLVVTPTCGLAGLTPSAAIERQRACLQVAAELVTRSRG"
     gene            1039..3195
                     /gene="ligA"
                     /locus_tag="PLB86_09265"
     CDS             1039..3195
                     /gene="ligA"
                     /locus_tag="PLB86_09265"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRC65362.1"
                     /translation="MRQDDAVSSSEAPAGAPDVPSEVRHAWTELAETIRAHQFAYHVQ
                     DSPTVSDGEYDALIRDLAALEESHPSLRTPDSPTQQVGAPSFSTEFQAVDHLERMLSL
                     DNAFSPEELAAWAARVERDAGGPGGAAYRFLCELKVDGLAINLLYERGRLTRALTRGD
                     GRTGEDVTHNVRTIRGIPAVLTPTAQAPVPELVEVRGEVFFPVEQFEELNASLVEAGR
                     APFANPRNAAAGSLRQKDPRVTASRPLRMLVHGLGARRGFDVARQSEAYAVLHDWGLP
                     TSPHVRVVDTVAEVQAFIDHFGEHRHSVEHEIDGVVVKVDEVAVQRSMGSTSRAPRWA
                     IAYKYPPEEVNTVLRAIEVGVGRTGRVTPFGVMDPVVVSGSTVSNATLHNGWEVARKD
                     VRIGDTVVLRKAGDVIPEIVAPVLALRPADAVPWEMPTHCPACGSLLAAEKEGDKDIR
                     CPNARSCPAQLRERLFGLASRGALDIEALGWEGAAALLDAGVIEDEGGLFALTAAEIA
                     TVPLYTRLAKKTDPEADVIDGRVLSANGEKLLDNLAKAKEQPLWRVLVALSIRHVGPT
                     AARALATHFGSMEGIRAASAEELSGVDGVGGVIAESVRDWFDAPDNTWHRAIVDAWAA
                     AGVRMVDAVDASVARTLDGATVVVTGSLEGFTRDGAKEAIIARGGKAAGSVSKNTDWV
                     IVGPGAGSKEAKARDLGLRILDEAGFVRLLETGSGD"
     gene            3261..3557
                     /gene="gatC"
                     /locus_tag="PLB86_09270"
     CDS             3261..3557
                     /gene="gatC"
                     /locus_tag="PLB86_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRC65363.1"
                     /translation="MSSLSRDDVAHLAMLARIELTDAELDRMLGELSVILDAVALVQQ
                     APTEGVEPMSHPLPLVNVTRPDEVRPGLTSAQALSGAPEADQQRFSVPRILSDD"
     gene            3680..5173
                     /gene="gatA"
                     /locus_tag="PLB86_09275"
     CDS             3680..5173
                     /gene="gatA"
                     /locus_tag="PLB86_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRC65364.1"
                     /translation="MADSLARREISAVELTQAHLDRIAAVDGSVHSYLHVDEAGVLAQ
                     ARAVDDARARNEDLHVMAGVPIAVKDVVATEGLPTTCGSRILSGWIPPYDATLVTKLK
                     AAGMPILGKTNMDEFAMGSSTEHSAYGPSRNPWDLERIPGGSGGGSSSVLASFQAPLA
                     IGTDTGGSIRQPASVTGTVGTKPTYGGVSRYGLVALASSLDQAGPCARTVLDTALLHA
                     VIGGYDPRDSTSIHAPVPEVVRAAQSADVSGMRIGVVKQLSGEGYQAGVQARFNEAVA
                     LLVDAGATVVEVDCPNFQYALAAYYLILPSEASSNLARFDAMRYGLRALPEGVDDPSA
                     EQVMAATREVGFGAEVKRRIILGTYALSSGYYDAYYGQAQKVRRLIAQDFDRAFEVAD
                     VLVSPTAPTTAFKIGEKLDDPLAMYLNDVATIPANLAGIPGMSLPSGLATEDGLPTGF
                     QILAPATKDERLYAVGSALERELVSRWGGHLLEKAPTLATVGAEVAR"
     gene            5170..6729
                     /gene="gatB"
                     /locus_tag="PLB86_09280"
     CDS             5170..6729
                     /gene="gatB"
                     /locus_tag="PLB86_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRC65365.1"
                     /translation="MSEHTSDDLALDGGTEAVLTWDETIAAYDPVLGLEVHVELGTAT
                     KMFCGCPTEFGAEPNSQVCPVCLGLPGSLPVVNEKGVESAIRIGLALNCTIAPWSRFA
                     RKNYFYPDMPKNFQTSQYDEPIAFNGWLDVELEDGSAYRVEIERAHMEEDTGKSLHVG
                     GATGRIHGADYSLVDYNRAGIPLIEIVTKPLTGFGDRAPEVAKAYVATLRDLLRALGV
                     SDVKMEQGSLRCDANVSLRPRKKKPEKQAEVPFGTRTETKNVNSLRSVERAVRYEMTR
                     HAAVLAGGGSILQETRHWHEDTGVTTSGREKSDAEDYRYFPEPDLVPIAPTADSIEAL
                     RATLPEPPVQRRRRLQGEWGYSDLEMRDVINAGAIEIIEATVAAGATPAGARKWWSGE
                     IARRANLDGRELTDMGITPRAVAELDALVQGGRLNDSMARQVLDGVLAGEGNPGAVAD
                     ARGLALVSDDGALGAAVDEVIAANPDLVEKIRSGKVQAAGALIGQVMKAMAGQADAAR
                     VRELIMERVGS"
     gene            complement(6811..>7155)
                     /locus_tag="PLB86_09285"
     CDS             complement(6811..>7155)
                     /locus_tag="PLB86_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65366.1"
                     /translation="GSSSGAGGGGLGDILGQVLGGSGTSAGSADSADSGLGLPEQPTG
                     PLIPTDGSDMSDVTDGSSNRSTGAQDNPMGGVLGDILGQVLGGGSSSGSGSSSSPNIT
                     DILGGLLGGGKR"
BASE COUNT         1037 a         2562 c         2497 g         1059 t
ORIGIN      
        1 ggaccgatgt cgccgaggcc ctgcggatcc agcgggagtt gctcggcgat cacgacgagc
       61 aggcggacgg ggtgcgcggc ataccgtcgc tgcctgagtt gcccgctcgc ggaccgggcg
      121 gggacatcat cggccgcacc gccgggttgc tcgtggacct cgccgtcgat ttgcaaccgg
      181 cgggatggcg tttcgtcgat cgaccgggcc gagacgctgc gcggacggcc gccttctggg
      241 gcgaggacct cgacgagttg gccgccgcct tcgacgggta tgccggtcca ctcaaggtcg
      301 cggtggccgg accgttcacc ctcgcggcgg acatttggct gcatcgcggc gaacgcgcca
      361 tcgtcgatcc gggtgccacc cgcgacctgt ccgagtcact cgccgaggga ctgcgcaccc
      421 tcctggtccg actgacccgg ctcgtgcctg gggctgagct catcgtccag gtcgacgagc
      481 cctccctgcc tgcggtgctt gacgggcgat tgcccacggc atcggggttc ggcaggttgc
      541 gggccatcga cgtccaggac gcgctacctg ccctgcagac ggttctggcg gcggcgggcg
      601 agcacgggac gatcgtgcat tgctgcgcgg cataccctcc gctgcccctg ctgcgccgaa
      661 gtggcgcggg cgggctctcg gtcgacacga cgctgatcac tccgcgcggc tgggagggcc
      721 tggccgtggc ggtcgaggac ggggttcgcc tgcacgccgg ctgtgtgccc accgcacccg
      781 aggcggtggt ggaccctgtc gccgtcgccg accacctcgc ctcggcctgg tcgcgcgtcg
      841 gtatgccgcc cgctcacctg gacgacctgg tcgtcacccc gacctgtggg ctggcggggt
      901 tgaccccgtc cgccgccatc gaacgccaac gcgcctgcct gcaggtggcc gcggagctcg
      961 tcacccgctc ccggggctga gcgaccgcac cccgccgtca agcgcccgtc gccgcccgtc
     1021 gaggggattg tcggcgcgat gcggcaggat gacgccgtgt ccagctccga agcgcccgca
     1081 ggcgcccccg acgtgccatc cgaggtccgg catgcctgga ccgagttggc cgaaacgatc
     1141 cgagcccacc agttcgccta tcacgtccag gattccccga ccgtgtccga cggggagtac
     1201 gacgcgctca ttcgcgacct cgcggccctg gaggagtccc accccagcct gcggacgccg
     1261 gacagcccga cccaacaggt gggagcgccg agcttctcga ccgagttcca ggcggttgac
     1321 cacctcgagc ggatgctcag cctcgacaac gccttctcgc ccgaggagct ggcggcctgg
     1381 gccgcgcggg tggagcggga cgccgggggg cccggcggcg cggcataccg gttcctctgc
     1441 gagctgaagg tcgacggcct ggcgatcaac ctcctctacg agcgcggccg cctcacccgc
     1501 gcgctcaccc gcggcgacgg gcgcaccggc gaggacgtca cccacaatgt ccgcacgatc
     1561 cgcggcatcc ccgccgtgct cacgcccacc gcccaggcgc cggtccctga gctcgtcgag
     1621 gtgcgcggcg aggtcttctt ccccgtcgag cagttcgagg agctcaacgc gagcctcgtc
     1681 gaggccggcc gcgcgccgtt tgccaacccc cgcaacgccg ctgccggttc gctgcgccag
     1741 aaggacccgc gggtcaccgc gagccgcccc ctgcggatgc tcgtccacgg gctcggggct
     1801 cgtcgcggct tcgacgtggc ccgccagagc gaggcgtatg ccgtgctgca cgactggggg
     1861 ctgcccacct ccccgcacgt gcgggtcgtc gacaccgtcg ccgaggtcca ggcgttcatc
     1921 gaccacttcg gagagcaccg acactcggtc gagcacgaga tcgacggggt ggtcgtcaag
     1981 gtcgacgagg tcgccgtcca acggtcgatg ggctcgacct cgcgtgcgcc gcgctgggcg
     2041 atcgcttaca aatacccgcc ggaagaggtc aacacggttc ttcgggccat cgaggtcggg
     2101 gtcggtcgca ccggtcgagt gaccccgttc ggcgtgatgg atcccgtggt cgtcagcggc
     2161 tcgaccgtct ccaacgccac cttgcacaac ggctgggagg tggcccgtaa ggacgtccgg
     2221 atcggtgaca cggtcgtgct gcgcaaggcc ggcgacgtca tccccgagat cgttgccccc
     2281 gtcctggcgt tgcgtccggc cgacgccgtc ccgtgggaga tgccgacgca ctgccccgcc
     2341 tgcgggagcc tgctggcggc cgagaaggag ggcgacaagg acatccgttg ccccaacgcc
     2401 cggtcctgcc cggcccagtt gcgcgagcga ctgttcggcc tggcctcccg gggcgccctg
     2461 gacatcgagg ccctcggctg ggagggggcg gctgcactgc tggacgctgg tgtcattgag
     2521 gacgaagggg ggctcttcgc gctcacggcc gccgagatcg ccaccgtgcc gctctatacc
     2581 cggctggcca agaagaccga ccccgaggcc gacgtgatcg atggccgggt cctgtcggcc
     2641 aacggcgaga aactgctcga caacctcgcc aaggccaagg agcagccgct ctggcgggtg
     2701 ctcgtcgccc tgtcgatcag gcatgtcggc cccactgcgg cccgcgccct cgccacccac
     2761 ttcggctcga tggagggcat ccgggccgcg agcgccgagg aactctcggg cgtcgacggg
     2821 gtgggcggcg tgatcgccga gtcggtgcgc gattggttcg acgccccgga caacacctgg
     2881 caccgggcca tcgttgatgc ctgggcagcg gccggggtcc ggatggtcga cgcggtcgat
     2941 gcatcggtgg cccgcaccct ggacggcgcc accgtcgtcg tcaccggctc cctcgaaggc
     3001 ttcacccgcg acggcgcgaa agaggcgatc atcgcccgcg ggggcaaggc ggccggatcg
     3061 gtctccaaga acaccgattg ggtgatcgtc gggccgggtg ccgggtccaa ggaggccaag
     3121 gcccgcgact tggggctgcg catcctcgat gaggcagggt tcgtccggct cctggagacc
     3181 gggtctggcg actaacggcc agggtgggct aagccgaacg tcccgcaagg gtcctcaggc
     3241 agcgccaata ggatggggcc atgtcttccc tgtcccgcga cgatgtcgcc catctggcca
     3301 tgctggcgcg gatcgagctg accgacgccg agcttgaccg gatgctcggc gagctgtcgg
     3361 tcatcctcga cgccgtcgca ctggtccagc aggcgcccac cgaaggcgtc gagccgatgt
     3421 cgcacccctt gcccctggtc aacgtcacca ggcccgacga ggtccgcccc gggctcacct
     3481 ccgcgcaggc cctctcgggg gctcccgagg ccgatcagca gcgcttctcc gtcccccgga
     3541 tcctctccga cgactgaccc accgcctctg gtatgccgcc tcgcggccca ggcgcgcggc
     3601 atacctgcag cttccgaccc gctccccaac gtaaggcacc accgtgacca acgatctcac
     3661 ccgctggagc gccgcccgga tggccgactc tctggcccgt cgggagatca gcgccgtcga
     3721 gctcacccag gcccacctgg accgcatcgc ggctgtcgat ggctccgtcc actcctacct
     3781 gcacgtcgac gaggctggcg tgctcgctca ggcgcgcgcc gtcgatgatg cccgcgcccg
     3841 caacgaagac ctgcacgtga tggctggcgt gccgatcgcg gtcaaggacg tcgtggcgac
     3901 cgaggggctg ccgacgacgt gtggcagccg gatcctctcg gggtggatcc cgccgtacga
     3961 cgcgaccctc gtgaccaagc tcaaggccgc cgggatgccg atcctcggca agaccaacat
     4021 ggacgagttc gcgatgggct cctcgacgga gcactcggcc tatggcccca gccgcaaccc
     4081 gtgggacctc gagcgcatcc ccggcggctc cggcggcggc tcgtcgtcgg tgctggcatc
     4141 cttccaggcc ccgttggcga tcggcaccga caccggtggc tcgatccgtc agccggcgtc
     4201 ggtgacgggg acggtcggca ccaagccgac ctacggcggg gtctcgcgct acggcctcgt
     4261 cgcgttggcc tccagcctcg accaggccgg gccgtgcgcc cggaccgtcc tggacaccgc
     4321 gctgctgcac gccgtcatcg ggggttacga cccgcgcgac tcgacgtcca ttcacgcccc
     4381 ggtgcccgag gtcgtgcggg cggcgcagag cgctgacgtc agcgggatgc ggatcggggt
     4441 cgtcaagcag ctgtccggcg agggctacca ggcgggggtg caggcccgct tcaacgaggc
     4501 ggtcgccctg ctcgtcgatg ccggcgcaac ggtcgtcgag gtcgactgcc ccaacttcca
     4561 gtacgcgctg gccgcctact acctcatcct gccgtccgag gcgtcctcca acctggctcg
     4621 cttcgacgcc atgcgctatg gcctgcgggc cctgcccgag ggagtcgatg acccgagcgc
     4681 cgagcaggtc atggcagcca cccgcgaggt cgggttcggc gccgaggtca agcgccggat
     4741 catcttgggc acctatgccc tctcgtcggg ctactacgac gcttactacg gtcaggccca
     4801 gaaggtccgt cgcctcatcg cccaggactt cgaccgcgct ttcgaggtgg ccgacgtcct
     4861 cgtctccccg accgcgccga cgaccgcgtt caagatcggc gagaagctcg acgacccgtt
     4921 ggccatgtat ctcaacgacg tcgcgacgat cccggccaac ctcgccggca tcccggggat
     4981 gtcgctgccg tccggtctcg cgaccgagga cgggctcccg accgggttcc agatcctcgc
     5041 cccggcgacg aaggacgagc gcctgtatgc cgtggggtcg gctctggagc gtgagctcgt
     5101 cagccggtgg ggcgggcact tgttggagaa ggcgccaacc ttggcgaccg tgggcgcgga
     5161 ggtggctcga tgagcgagca cacgagtgat gacctggccc tcgacggcgg caccgaggcc
     5221 gtgctgacgt gggacgagac gatcgcggca tacgacccgg tgctcgggtt ggaggtccac
     5281 gtcgagttgg gcactgcgac caagatgttc tgcggttgtc cgaccgagtt cggtgccgag
     5341 cccaactctc aggtgtgccc ggtctgtctc gggctgccgg ggtcgctgcc ggtggtcaac
     5401 gagaagggcg tcgagtcggc catccggatc gggctggcgc tcaactgcac catcgcgccg
     5461 tggagccggt tcgcccggaa gaactacttc tatccggaca tgcccaagaa cttccagacc
     5521 agccagtacg acgagccgat cgccttcaac gggtggctcg acgtcgagtt ggaggacggc
     5581 agtgcctacc gggtcgagat cgagcgggcc cacatggagg aagacaccgg caagtcgctg
     5641 cacgtcggcg gcgccaccgg gcgcatccac ggtgccgact actcgctggt cgactacaac
     5701 cgagccggca tcccgctcat cgagatcgtc accaagccgt tgaccggctt cggtgatcgc
     5761 gcgcccgagg tggcgaaggc ctacgttgcg accctgcggg acctgttgcg ggcccttggc
     5821 gtgtccgacg tcaagatgga gcaggggtcg ctgaggtgtg acgccaacgt ctcgcttcgc
     5881 ccgcgcaaga agaagcccga gaagcaggcc gaggtcccct tcggcacccg caccgagacc
     5941 aagaacgtca actcgttgcg gtcggtggag cgggctgttc gttacgagat gacccggcat
     6001 gcagcggttc tggccggtgg cggttcgatc ctgcaggaga cccggcactg gcacgaggac
     6061 accggggtca cgaccagtgg ccgcgagaag tccgacgccg aggactaccg ctacttcccc
     6121 gagcccgacc tggtccccat cgctccgacg gccgactcga tcgaggcatt gcgggcgacc
     6181 cttcccgagc cgccggtgca gcgtcgccga cggctgcagg gggagtgggg ctactccgac
     6241 ctggagatgc gcgacgtcat caacgccggt gccatcgaga tcatcgaggc caccgtggcc
     6301 gccggtgcga ccccggccgg tgctcgcaag tggtggtcgg gcgagatcgc ccggcgcgcc
     6361 aacctcgacg gccgcgagct caccgacatg ggcatcacgc cgcgtgctgt cgccgagttg
     6421 gacgcgctcg tgcagggcgg ccgactcaac gactcgatgg cccgccaggt gctcgacggt
     6481 gtgctcgccg gcgagggcaa ccccggtgcg gtcgccgatg cccgtggtct ggcgctcgtg
     6541 tccgacgacg gcgcgctggg tgccgcggtc gatgaggtca tcgccgccaa ccccgacctc
     6601 gtcgagaaga tccgcagcgg caaggtccag gccgccggcg ccctcatcgg tcaggtcatg
     6661 aaggcgatgg ccggtcaggc cgacgccgcg cgggtccgcg agctcatcat ggagcgggtc
     6721 ggcagctagg acgcacgctc caccccacac acaccagcgg gtatgccgcg cgcggacgtg
     6781 ccgcgcggca tacccgctgc gggtggtggg tcagcgctta ccgccgccga gcagcccgcc
     6841 gaggatgtcg gtgatgttgg ggctgctgct ggacccggag cctgacgagc taccgccgcc
     6901 gaggacctga ccgaggatgt cgccgagcac gccacccatc gggttgtcct gcgcgccggt
     6961 ggagcggttg ctcgagccgt cggtgacgtc ggacatgtcg ctgccgtcgg tcgggatgag
     7021 gggaccggtg ggctgttcgg gcagccccaa acccgaatcg gccgaatcag ccgagccggc
     7081 cgacgtgccg gagccgccga ggacctgacc gaggatgtca ccgagaccgc cgccgcccgc
     7141 accggacgag ctgcc
//
LOCUS       DAORPW010000114         7153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2689_length_7153_cov_809.663849, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000114 DAORPW010000000
VERSION     DAORPW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2689_length_7153_cov_809.663849"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2062)
                     /locus_tag="PLB86_09290"
     CDS             complement(<1..2062)
                     /locus_tag="PLB86_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65367.1"
                     /translation="MILTQEVLSRWLHRGACALTGVFVVAVPGLQLIDPVAYPPLLFQ
                     TWLVLALATLGWALWDAYRCARVPWGLRAAVAVAIAAGLTQMVGGWRNSDSLTIPPLL
                     QAMAPAFGVVGFALSPRAAVPIGLICGTAFAVSEWGVDPGMPAVSSGLVVLGSGLIAG
                     GVVSLLYRAAAKVEQALDARWDAREAIARSVVRAEQTQLWDGLVHDKVLGALRLAARA
                     RDDSDRRAAGELAREALITGSAPATLGGGGSGRALRSRVERVCHRLGLQLRWTVEERD
                     GSGPTDGAAAAVLAAAEEALVNVSRHTGCRAVTIAGSVGGDLRLDIIDAGPGFDLLAP
                     RPGRLGIDRGIIGRLSRVGGSAQVLTRPGHGTTVRLVVPAAGRDLRPQLPPPLETWRQ
                     QDFVGLFLMGVALTGVYAAAAIATVDRTRSAGVVAICLALVVALGLTASLAPGHRDDV
                     AALIVVGVGVVCALGMWNLRRPFDEGWATWFVGALNATVVVLTGRFRTRWGFLAVASA
                     ALGLAVGQVLAAGVVHLGVITALVPQQVAFVCAIWGVRRALDLATSAINEAAAAGGEV
                     RLADAQAEEAAEVAQARSRGVLAAAGPLLERISAREAFDAAGRERCLLAEAEVRDGLV
                     AAPLLTPELIATLRDCRQRGVIVAVTAEEREGDVGLAEFRGILGSILAVAPIGSRVNA
                     RLRPD"
     gene            complement(2059..2730)
                     /locus_tag="PLB86_09295"
     CDS             complement(2059..2730)
                     /locus_tag="PLB86_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC65368.1"
                     /translation="MSEGLRLGVVDDHPAILRGILTILVELCPEITETFTTSTVPALL
                     GAHRSLDIVVLDVRLADGSDPAANVAAVVDAGLPVLLYTQEQRRGVLARCFRAGALGV
                     VDKSEEPGVLAAAIRVVAAGEPYLSSGWAAAVEDADDWPVPELAPRESEALGLYATGL
                     PLKSVARRMHLSEDTVKEYLDRVRRKYTRAGRPAYTKVDLYQRAVEDGYLPPPGEGAP
                     ADPAR"
     gene            complement(2979..4169)
                     /locus_tag="PLB86_09300"
     CDS             complement(2979..4169)
                     /locus_tag="PLB86_09300"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRC65369.1"
                     /translation="MTWLGVVASAALNYRRGSVPLIWWVLCVIVLVTFLALGGSTPRT
                     STRDRVLFTILTLSAVAACGVWGADLMSGVLLVLVAGVAGWVVPPKVSIPLMLAMTAI
                     LAFFMWTAKSTVVWALLYGSLLFFASLMVSVVVREQAARRIAATVAAELEVANARLLG
                     ANDALRVAHGRLAEASKAEERLRISRDLHDGMGNQLTALSLQLDLIGRLVDGAAVTHL
                     DQAKALTTELIRDTRGVVSQLRDTRVSPRDKIARLARSMPRPQTSLDLTEALDEVPEP
                     VAETVVRLVQEGLTNAARHADAEHAWVVVSCAPDGLRLQVRDDGRGAPTLVPGNGLSG
                     MAERVALLGGDLTWSSPSSGGFIVTASIPVTQASPDTPVEAAGPDVPDTHNTGSPMSD
                     SVVG"
     gene            complement(4327..4797)
                     /locus_tag="PLB86_09305"
     CDS             complement(4327..4797)
                     /locus_tag="PLB86_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC65370.1"
                     /translation="MLSDQRAGGRRAAYQPSDDSPVFVISVAAQLAGMHSQTLRQYDR
                     LGLVTPSRTRGGGRRYSTRDVALLREVQRLSQEEGISLAGIQRILRLEHEVAALRARV
                     SELTDELGAARHTVGHRVFAAGPAGDIVAMRPGQRPSRPAGPSRALVVWRPQRG"
     gene            complement(4833..5891)
                     /locus_tag="PLB86_09310"
     CDS             complement(4833..5891)
                     /locus_tag="PLB86_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRC65371.1"
                     /translation="MASQDWLSKDFYAVLGVPKEADEAAIKKAYRKLARKLHPDQNAG
                     DPKAEERFKEIGEAYAVLSDPDERHQYDQLRAMTTGGARFTAGPGGAGGGFEDVFSGM
                     FGNGGNMRFNTGGRGGAGGAGPTPDLQDLLGGLFGGAGQGGAGGFGGDPYGGFGGDPY
                     GGYGAPRGPRRGADVSARTTLDFRQAVQGDTVTLATGDGDRLTTRIPAGVKDGQKIRL
                     RGKGQPGGPGAERGDLILTVAVTPHPVFGRDGDNLTVDLPVTFAEAALGATVSVPTLS
                     GTAVRVKVAPGTPSGRVLRIKGRGVKHAKGDGDLLAKVQVIVPQRLTDEAREALERLR
                     DQEEGLDPRAELFAKAAH"
     gene            complement(5928..6605)
                     /gene="grpE"
                     /locus_tag="PLB86_09315"
     CDS             complement(5928..6605)
                     /gene="grpE"
                     /locus_tag="PLB86_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRC65372.1"
                     /translation="MTHGHEHDAEGRGHHGPAHPGDSGSAEPGGFDEFDHPDFADIAA
                     AAAASLREEHPEGTGIPVVSDGTAAQGDGAEPSLAPLTGDAALAAERLEDLRRLQAEY
                     VNYKKRVDRDRSVARDQGISHVLESMLPVLDEIHLAREHGDLGEGPAATIVDKLEAVL
                     AKLGIERFGAAGDEFDPNHHEALMRMEADLPEGATTTTVVQVLQPGYRLGERIVRAAR
                     VAVADPQ"
     gene            complement(6602..>7153)
                     /locus_tag="PLB86_09320"
     CDS             complement(6602..>7153)
                     /locus_tag="PLB86_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRC65373.1"
                     /translation="PRGIPKIEVTFDIDANGIVHVSAKDMGTGKEQSMTISGGSALPK
                     EEIERMVREAEQHAEEDRQRVEEIETRNQAEQVVYSTEKFLADNADKIPADVKGEVET
                     ALADVKTVIAKDSTASVEDIKAKVATLDEVRSKMGQAMYAAAAASGEGDGSADAGPQD
                     AESAAGPQDDVVDAEVVEDEDKK"
BASE COUNT         1136 a         2597 c         2423 g          997 t
ORIGIN      
        1 ggtcagggcg aagtcgcgcg ttgacccgcg agccgatcgg ggccaccgca aggatgctgc
       61 cgaggatccc tcggaactcc gccaggccga catcgccttc gcgttcctcg gcggtgacgg
      121 cgacgatcac gccccgctgt cggcaatcgc gcagcgtcgc aatcaattcg ggggtcagca
      181 gcggcgcggc aactaaccca tcccgcacct cggcctcggc cagcaggcac cgctcgcggc
      241 cagccgcatc gaaggcctcc ctggcggaga tccgctccag cagggggccc gcagcggcca
      301 ggacaccccg gctgcgggcc tgggcgacct cggccgcttc ctcggcctga gcgtcggcca
      361 ggcgcacctc tccccccgcg gcggcagcct cattgatcgc gctcgtggcg aggtcgagag
      421 cccggcggac cccccagatc gcgcacacga aggcaacttg ttgcggcacc agggccgtga
      481 tgacgccgag gtggacgaca ccggccgcca gaacttggcc caccgcaagg ccgagcgcag
      541 cactggcgac ggcaaggaac ccccaccggg tccggaaccg cccggtcagc acgacgacgg
      601 tggcgttgag cgccccgacg aaccaggtgg cccacccctc gtcgaacggt ctgcgcagat
      661 tccacatccc cagtgcgcag accactccca ccccgacgac gatcagtgcg gccacgtcgt
      721 cccgatggcc cggggccaac gaggccgtca accccagcgc cacgaccaag gcaaggcaga
      781 tcgcgacgac gcccgccgac ctcgtccggt cgaccgtggc gatggcggca gcggcataga
      841 ccccggtgag ggcaactccc atcaggaaga gcccgacgaa gtcctgctgc cgccacgtct
      901 caagcggtgg cggcagctga ggccggagat cacgaccagc agcgggcacc accaaccgga
      961 ccgtcgtgcc atgcccggga cgggtgagga cctgagcgga ccccccgacc cgggacagcc
     1021 gtccaatgat gcctcgatcg atgcccaggc ggccggggcg cggggccagc aggtcgaagc
     1081 ccggtccggc gtcgatgatg tcgaggcgca ggtcaccgcc gaccgaccct gcgatggtga
     1141 cggcccggca tccagtgtgt cgggacacgt tgaccagcgc ctcttcagca gcggccagga
     1201 ccgctgcagc ggcaccatcg gtcggccccg agccgtcgcg ttcctccacc gtccaccgca
     1261 gctgaagtcc gagccgatga cagactcgct caacgcggga tcgcaaggcg cgcccggagc
     1321 cgccgccgcc gagggtagcc ggggcgctac cggtgatgag cgcctcgcgc gcaagctccc
     1381 ccgccgcccg tcggtcgctg tcgtcccgcg ctcgggccgc caaccgcaga gcacccaaca
     1441 ccttgtcatg aacgagtccg tcccacagct gcgtctgctc agccctgacc acagagcggg
     1501 cgatcgcttc ccgggcgtcc cagcgcgcgt ccaacgcctg ctcgaccttt gccgcggctc
     1561 ggtagaggag actgacgacc ccacccgcga tcagccccga cccgaggacg accaggccgg
     1621 aagacacggc gggcatcccc ggatcgacgc cccactcgct gaccgcgaac gcggtgccgc
     1681 agatcagccc gatgggaacc gccgcgcgag gagagagcgc aaaccccacg acaccgaagg
     1741 caggggccat ggcttgcagc agcggaggga tggtcaacga gtcgctgttg cgccagcccc
     1801 ccaccatctg cgtcaacccc gcggcgatgg cgaccgcgac tgctgctctc aggccccaag
     1861 ggacgcgagc gcagcggtag gcgtcccaca aggcccagcc cagcgttgcg agggccaaca
     1921 cgagccacgt ttggaagagc agcggcggat acgccaccgg atcgatgagc tgcagacccg
     1981 ggaccgcgac gacgaagaca cccgtcagag cgcacgcgcc gcgatgcaac caccggctga
     2041 ggacctcttg ggtcaggatc atcgggccgg gtcggcgggc gcgccctccc ccgggggcgg
     2101 caggtagccg tcctccaccg cccgctgata gaggtcaacc ttcgtgtatg ccggtcggcc
     2161 ggctcgcgtg tatttgcgcc gcacccgatc gaggtactcc ttgaccgtgt cctccgacag
     2221 atgcatgcga cgagcgacgg acttgagcgg aaggcccgtg gcgtagagcc ccaaggcctc
     2281 gctctcgcgc ggagccagct cggggaccgg ccaatcgtcg gcgtcctcca ccgctgcggc
     2341 ccagccggag ctgaggtagg gctctcctgc agcgacgacg cggatcgccg ccgcgaggac
     2401 gcccggctcc tcgctcttgt cgaccacgcc cagggccccg gctcggaagc accgggccag
     2461 cacaccgcgg cgttgctcct gggtgtagag caggacggga aggccggcat cgaccaccgc
     2521 cgcgacgttg gcggccgggt cactgccgtc agccaggcgc acgtcgagga ccacgatgtc
     2581 gaggctgcga tgagccccga ggagcgcggg gaccgtcgag gtcgtgaacg tctcggtgat
     2641 ctccgggcac aactcaacaa ggatcgtgag gattccgcgc agaatcgccg ggtgatcgtc
     2701 aacgacaccg agccgcagcc cctcgctcat actgcgagtc tgccattagc gacaattctc
     2761 atagcacgtg ggaatgtccc tgaaacgggg gacttactcc agcgcggaga ttggagcagg
     2821 ctaggtattg cgtcgcaggg gggattcgag cctgcggagg cgctcgagtc tcgagttgac
     2881 gtgacggtcg gagggtatgc cgccccgctg gggcaagccg ggcggcatac cctctgccac
     2941 gtcgagggag ctccgactcg caggctcgct gcctggactc agccgaccac gctgtcggac
     3001 atcggcgacc cagtgttgtg agtgtcgggg acatccgggc cggccgcttc gaccggtgtg
     3061 tccggggacg cctgggtcac cgggatcgag gccgtcacga tgaacccgcc gctcgacggg
     3121 cttgaccacg tcaggtcacc gccgagcagt gcgacccgct cagccatccc ggacaggccg
     3181 ttccccggga ccagagtggg agcgcccctg ccgtcgtcgc ggacctgcaa acgcagcccg
     3241 tcgggcgcac agctgaccac gacccaggca tgctcagcat ccgcgtgcct ggcggcgttg
     3301 gtcaacccct cctggaccag cctgacgacg gtctcggcca ctggctcggg gacctcatcg
     3361 agggcttcgg tcagatcgag cgaggtctgg ggccggggca tcgagcgggc caggcgagcg
     3421 attttgtcgc gaggggacac ccgcgtgtcg cgcaactggc tgacgactcc ccgcgtatcg
     3481 cggatcaact cggtggtcag tgccttggcc tggtcgaggt gggtgaccgc ggcgccgtcg
     3541 acgagccgtc cgatcaggtc cagctgcaga gagagtgccg tcagttgatt gcccatgccg
     3601 tcgtgcaggt cgcgcgagat gcgcaggcgc tcctccgcct tgctcgcctc ggccagccgg
     3661 ccgtgggcaa cacgcagggc atcgttggcg ccgagcaggc gggcgttggc cacctcaagc
     3721 tccgcggcga cggtggcggc gatccgccgg gcagcttgct cacggacgac caccgagacc
     3781 atgagcgagg cgaagaacag caggcttccg tagagcagcg cccacaccac cgtggacttc
     3841 gccgtccaca tgaagaaggc gaggatcgcc gtcatcgcga gcatgagcgg gatcgagacc
     3901 ttgggcggga cgacccaacc ggctacgccg gcgaccagga caagcaggac ccccgacatc
     3961 aggtcggcgc cccacacccc gcaggcggcg acggcggaca gggtgaggat cgtgaagagc
     4021 acccgatcgc gggtcgaggt ccgaggggtc gaaccaccga gggccaagaa ggtcaccagg
     4081 acgatgacgc agagcaccca ccagatcagc gggacggaac cccgacggta attcagggca
     4141 gcgctggcca ccaccccaag ccaggtcacc gccccaccga gtgcggagcc ccaagcgacg
     4201 aaatcgttct ttcgccacgg gaccgcccgc caatcgccgc ccgccacagg gccagagtag
     4261 gaggcggcag ggggatttac cagagcaccc aggcctattc gcgggcgccg ttgcagacgg
     4321 tccgggtcag ccgcgctggg gtcgccaaac gaccagcgcc cgcgacggcc ccgcagggcg
     4381 cgacggtcgc tgacccggac gcatcgcgac gatgtcgccc gccggtcccg ctgcgaacac
     4441 ccggtgcccg acggtatgcc gcgccgcgcc gagttcgtcg gtcagctcac ttactcgcgc
     4501 ccgcagcgcc gcgacctcat gctcgaggcg caggatgcgc tggatgccgg ccaggctgat
     4561 gccctcttcc tgcgagagcc gctgcacctc gcgcaggagc gcgacgtcgc gcgtggagta
     4621 acgccgccca ccaccccttg tccgcgacgg cgtcacgagc ccgagccggt cgtactggcg
     4681 cagggtctgc gagtgcatcc cggccagttg cgcggcgacc gagatgacga acacgggaga
     4741 gtcgtcggac ggttggtatg ccgcccgccg acctcccgcg cgctggtcac tcaacatgct
     4801 ggtcaccgac catgggggtc accttccggg cctcagtggg cggccttggc gaagagctcg
     4861 gcccgggggt cgaggccctc ctcctggtca cgaagccgct cgagggcctc gcgggcctcg
     4921 tcggtgaggc gttgcgggac gatcacctgg accttggcca gcagatcacc gtcacccttg
     4981 gcgtgcttga ccccacgacc cttgatgcgc agcacccggc cactgggcgt gcccggcgcg
     5041 accttgaccc gcaccgcggt tccgctgagg gtcgggaccg acacggtggc accgagcgcc
     5101 gcctcggcga aggtcaccgg caggtcgacc gtgaggttgt caccgtcgcg cccgaacacc
     5161 gggtgcgggg tgacggcgac ggtgaggatg agatcgcccc gctcagcgcc gggtcccccc
     5221 ggctgaccct tgccgcgcag cctgatcttc tgaccgtcct tgaccccggc cgggattcga
     5281 gtggtcagtc gatcaccgtc accggtggcc agcgtcaccg tgtcgccctg gacggcctga
     5341 cggaagtcga gcgtcgtgcg tgccgacacg tcggcgccgc gtcgcggacc ccgaggggcg
     5401 ccgtaaccgc cgtaggggtc accgccgaag cccccgtacg ggtcaccgcc gaagccgccg
     5461 gcgccaccct gtccggcccc gccgaagagg ccgccgagca ggtcctgcag gtcgggcgtc
     5521 ggaccggcgc caccggcacc cccccgccca cccgtgttga accgcatgtt cccgccgttg
     5581 ccgaacatcc ccgagaagac gtcctcgaag ccaccgccgg caccacctgg cccggccgtg
     5641 aaccgggcac cgccggtggt catcgcccgc aactggtcgt actggtgacg ttcgtcggga
     5701 tcggacagca cggcatacgc ctcgccgatc tccttgaagc gctcctccgc cttggggtca
     5761 ccggcgttct ggtcggggtg cagcttgcgg gccagcttgc ggtaggcctt cttgatggcg
     5821 gcctcgtcgg cctccttggg cacgccgagc actgcgtaga agtccttcga caaccaatcc
     5881 tgactagcca attcgacgcg ccctcctctc agtgaaaacg gttggtgtta ttggggatcc
     5941 gcgaccgcaa cgcgggccgc gcgaacgatc cgttcgccca gtcgataacc gggttggagc
     6001 acctggacga ccgtggtggt cgtggcgccc tccggcaggt cggcctccat ccgcatgagc
     6061 gcctcgtggt ggttggggtc gaactcgtcc cccgccgcac cgaaccgctc gatgcccaac
     6121 ttggccagca ccgcctcaag cttgtcgacg atcgtggctg ccggcccctc gccgagatcg
     6181 ccatgctcgc gggcgagatg gatctcgtcg agcaccggca gcatcgactc gaggacatgg
     6241 ctgatgccct ggtcgcgcgc aacggaccgg tcccggtcga cgcgcttctt gtagttgacg
     6301 tattcggcct gcaaccgacg caggtcctcg aggcgctcgg ccgcgagggc cgcgtcgccg
     6361 gtcagcggcg cgagggacgg ctcggcaccg tcaccttggg cggcggtccc gtctgagacc
     6421 acaggtatgc cggtcccctc ggggtgctcc tcccgcaggc tcgcagccgc agccgccgcg
     6481 atgtcggcga agtccgggtg gtcgaactcg tcgaagccgc cgggctcggc cgagcccgag
     6541 tcgcccggat gggcggggcc gtgatggccc cgcccctccg cgtcgtgctc gtgcccgtgg
     6601 gtcacttctt gtcctcgtcc tcgacgacct cggcgtcaac cacgtcgtcc tgcggaccgg
     6661 cagcggactc ggcgtcctgc gggccggcgt cggcggaacc gtcgccctcc ccggaagccg
     6721 cggccgcggc atacatggcc tgccccatct tggagcgcac ctcgtcgagc gtcgcgacct
     6781 tcgccttgat gtcctcgacg gaagcggtcg agtccttggc gatcaccgtc ttgacgtcgg
     6841 cgagcgcggt ctcgacctca cccttgacgt cggccgggat cttgtcggcg ttgtcggcga
     6901 ggaacttctc ggtggagtag acgacctgct cggcctggtt gcgggtctcg atctcctcga
     6961 cgcgctggcg gtcctcctcg gcgtgctgct cagcctcgcg aaccatccgc tcgatctcct
     7021 ccttgggcag cgcgctgccg ccggagatcg tcatcgactg ctccttgccg gtgcccatgt
     7081 ccttggcgga cacgtgcacg atgccgttgg cgtcgatgtc gaaggtgacc tcgatcttcg
     7141 ggatgccgcg agg
//
LOCUS       DAORPW010000115         7145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2695_length_7145_cov_793.375882, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000115 DAORPW010000000
VERSION     DAORPW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2695_length_7145_cov_793.375882"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="PLB86_09325"
     CDS             <1..1391
                     /locus_tag="PLB86_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRC65374.1"
                     /translation="MPTLMGRDSDDPHSLGEVGHCGVAIDSAADMEVLFGGIPLGDVT
                     TSMTISGPAVPVFCMYLVAAERQGVDIGVLNGTLQTDIFKEYIAQKEWLFEPGPHLRL
                     IGDLMEFCTERIPDYKPLSVSGYHIREAGSTAAQELAFTLADGFGYVELGLSRGLDVE
                     TFAPGLSFFFDAHVDFFEEIAKFRAARRIWARWMRDVYGAKTDKAQWLRFHTQTAGVS
                     LTAQQPYNNVVRTAVEALAAVLGGTNSLHTNALDETLALPTEQAAEIALRTQQVIMHE
                     TGVTNVADPLGGSWYVEALTDRMEAEAEAIFTKIKDLGVLGRRPGPSGQIEHEIGPIT
                     SGLLRGIEEGWFMSEIADAAFQYQISLEKGDKKVVGVNLLTESVSHDLEILRVSHQVE
                     VDQVADLTARRSRRDEAAVQAGLAAMLAAARGGVNMIPAMLDAVRAEATLGEICDVLR
                     DEWGIYREPAQF"
     gene            1480..2547
                     /locus_tag="PLB86_09330"
     CDS             1480..2547
                     /locus_tag="PLB86_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65375.1"
                     /translation="MTLHHRSRAANLAALAVTGIALSVAVSACSSSTPSTASATSKAT
                     ARATATAKPSESTKPANGAVGAETPEQANAEVAKAITAQIAAAAVPGTKGKALREAAY
                     VDQALAAATAQGKLVSTLTAAQKADLALSPTDPVVLAVSRSAAYPRVILAKATFAGSG
                     SPALVTLVTPGADTAYRIASIARMLPSATVGKFDAVTAGSAPIGDAAGLAVAPDALLA
                     AYSAALAYPAPAAAEGPFTEDAFFAAVRKGAADQAASLGSAVTLKQTHAPAGVVAALR
                     AATGQGAYVVAVIERKDSLTEKTANALTPSKAFTILSGKSVINKSAVLSTYEFVVFHI
                     PASGKATVVAAAEQLYAASGT"
     gene            2569..3603
                     /locus_tag="PLB86_09335"
     CDS             2569..3603
                     /locus_tag="PLB86_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC65376.1"
                     /translation="MSSSPFTAAALRGAVDLSAFKRPANGPGARPGGAAGAGSAGSAG
                     AAGAAGGSGAPGASASSGGDLVVSVNDATFQDIATTSLRVPIVVVLWSARLAQTASFV
                     GVMADVARDNGGRFQVASVDVDSSPGLLRAFQIQSVPATIGLVQGQPVPLFVGALPPS
                     EIQPWIDELLKLAVQMGVTGRVSVGDDAPAAAEGADGVDGAEAAEDELPPLIAAAYDA
                     IERGDLDAAIAAYEEALVQNPADTEAALGLGQVRLLKRTEGVDAVAARAAAAADPLDV
                     AAQTVVADLDVLGGHVPDAFARLVDTVRATAGDDRNAARQHLLGLFEVVGASDERVVK
                     ARRALMSALF"
     gene            complement(3683..6169)
                     /locus_tag="PLB86_09340"
     CDS             complement(3683..6169)
                     /locus_tag="PLB86_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRC65377.1"
                     /translation="MDQVTTHVGHPGLTSAEVADRVRDGRTNDMPARSGRSTGEIVRA
                     NVLTRINAILGILLVIVLTTGKLINAAFGLLIIANSAIGIIQELRAKRTLDNLAVIGE
                     ARPTVRRDGVSRPVTRTDIVADDVIELGPGDQLIVDGDVLEAASLEVDESLLTGEADP
                     VVKGPGDQVLSGSFVVAGSGAYRATKVGREAYAARIVEEAAKFTLVGSELRDGINRIL
                     LVVSWLLVPVGLLTIWTQLFGLGQPWREGLTGMVAALVPMVPEGLVLMTSIAFAVGVI
                     RLGQRQCLVQELPAIEGLARVDVVCADKTGTLTESGMRLSEVRGFDGSGMPSGPAPAE
                     VTEILAQLTATEPNPNASLAAIAEAIAAPSVIWTETHHAPFTSARKWSGSTFDGQGTW
                     VLGAPDVLAPEGSAAREAAEEIGASGLRVLLLAEGSLPVTDPAAPGTVTPAALVILEQ
                     RVRPDARSTLEFFAEQHVQVKVISGDNARSVGAVTTLLGVVGGSDPIDARTLSQVDPD
                     DADTQAGQPAALGFVGGHHDQITHLSEAFVDAVARHTVFGRVTPDQKRAMVLALKSRG
                     HTVAMTGDGVNDVLALKKADIGVAMGAGSPATRAVAQIVLLDNKFATLPYVVAEGRRV
                     IGNIERVANLFLTKTVYSVLIALLVGIWQLPFPLQPIHVTITGWFTIGVPAFLLSLAP
                     NHERARPGFARRVLQLALPSGVVVAICTFTTYLLCRPPVGSGEALRTQASTASLACLI
                     IVATWVLSVVARPYEWWKVALLVLSVAAYVAIFLVPFTQSLFLLDAGNAAMMRTGLTV
                     GLAGAAAIEAIWWIQGRLRGEPRRWWVADD"
     gene            6240..6800
                     /locus_tag="PLB86_09345"
     CDS             6240..6800
                     /locus_tag="PLB86_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HRC65378.1"
                     /translation="MDVGARAQARRREGAGAVDLEGFVNAHYAQLLRRAYLLTGSRAD
                     AEDLVQEALARTWEAARGRAVESPGAYVHRTMVNLYVSRWRRRRLRREDPVAEIPEPV
                     AGGARHTADAVGEVDERDRLWRVLGSLPAKQRAVLVLRYYEDLSEGAAADLLGVTVGT
                     VRSQTWKGLRRLEAALGSRVEGDGRG"
     gene            6793..>7145
                     /locus_tag="PLB86_09350"
     CDS             6793..>7145
                     /locus_tag="PLB86_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65379.1"
                     /translation="MADLEAQVRATLADRAGRLEGPLPYAAPPALVDAVATRAAQLHR
                     RRTLVRVGGAAAAVAVIGLAAATLVRGPSVAGPLGPAGSVTATATATRTVDSATATAT
                     ATATATATRTVDSGTS"
BASE COUNT         1035 a         2467 c         2598 g         1045 t
ORIGIN      
        1 acatgccgac cctcatgggc cgcgactccg acgacccgca cagcctcggt gaggtcgggc
       61 actgcggtgt cgccatcgac tcggcggccg acatggaggt gttgttcggc ggcatacccc
      121 tgggtgacgt gaccacctcg atgacgatca gcgggcccgc ggtgccggtc ttctgcatgt
      181 acctcgtcgc ggccgagcgg cagggcgtgg acatcggcgt cctcaatggg accctgcaga
      241 ccgacatctt caaggaatac atcgcccaga aggagtggct cttcgagccc ggcccgcacc
      301 tgcggctcat cggcgatctc atggagttct gcaccgagcg gatcccggac tacaagccgc
      361 tgtcggtgtc gggctatcac attcgcgagg ccggctcgac ggccgcgcag gagttggcgt
      421 tcaccctcgc cgatggtttc ggttacgtcg agttggggct ctcgcgcggg ctggacgtcg
      481 agaccttcgc gccggggctg tcgttcttct tcgacgccca cgtcgacttc ttcgaggaga
      541 tcgcgaaatt ccgtgcggcg cgccggatct gggcccgctg gatgcgtgat gtctatggcg
      601 ccaagaccga caaggcgcaa tggctgcggt tccacaccca gaccgcgggt gtgtctctca
      661 ccgcgcagca gccctacaac aacgtcgtcc gcactgcggt cgaggcgctc gcggcggtgc
      721 tcggcggcac caactcgctg cacaccaacg ccctcgacga gaccctcgcc ctgccgaccg
      781 agcaggccgc cgagatcgcc ttgcgcaccc agcaggtgat catgcacgag accggcgtga
      841 ccaacgtcgc cgacccgctc ggcggctcct ggtacgtcga ggccctcacc gaccggatgg
      901 aggccgaggc cgaggcgatc ttcacgaaga tcaaggacct cggcgtgctg ggccgccggc
      961 ccggaccgtc cggccagatc gagcacgaga tcggcccgat cacctcaggc ctgctgcgtg
     1021 gcatcgagga gggctggttc atgtcggaga tcgccgacgc ggccttccaa taccaaatct
     1081 cgctcgagaa gggcgacaag aaggtcgtcg gcgtcaacct cctgaccgag tcggtgagcc
     1141 acgacctgga gatcctgcgg gtcagccacc aggtggaggt ggaccaggtc gccgacctga
     1201 ccgctcgccg ctcgcgccgc gacgaggccg cggtccaggc cggtctggcg gcgatgctcg
     1261 cggcggctcg cggcggcgtc aacatgattc ccgcgatgct cgacgcggtc cgcgccgagg
     1321 ccactctcgg cgagatctgc gacgtgctgc gcgacgagtg gggcatctat cgggagcccg
     1381 cacagttctg agcgggagtg cgagcggggg accgatttgg tccctcacgg gttgctgcgc
     1441 ctaccctgga cgaaccccac cgggaggatc agaccccaca tgacgctcca ccatcgctct
     1501 cgtgctgcca acctggccgc gctcgccgtg accgggatcg ctctgtccgt agctgtctcc
     1561 gcgtgctcgt catcgacccc gtcgacggcg tcggcgacca gcaaggcgac tgcgcgcgcg
     1621 acggcgaccg cgaagccgag cgagtcgacg aagcctgcca acggtgcggt cggcgccgag
     1681 acgccggagc aggccaacgc ggaggtcgcc aaggcaatca ccgctcagat cgccgcggct
     1741 gccgtgccgg ggaccaaggg gaaggccctg cgcgaagccg cctatgtcga ccaggccctg
     1801 gcggcggcca ccgcacaggg caagctcgtg agcactctga cggctgccca gaaggccgat
     1861 ctcgcgctga gccccacgga cccggtcgtc ctggcggtat cgcgtagcgc ggcatacccc
     1921 agggtgatcc tcgccaaggc gaccttcgcc gggagcgggt cacccgccct cgtgacgctc
     1981 gtgacgcccg gcgccgacac cgcctatcgg atcgcctcga tcgcgcggat gctgccgtcg
     2041 gcaacggtcg gcaagttcga tgcggtgacc gccggctcgg ccccgatcgg ggacgctgcg
     2101 ggtctggccg tcgccccgga tgccctgctc gcggcatact cggcggccct ggcctatccc
     2161 gcgcctgccg cagccgaggg gccgttcacg gaggacgcct tcttcgcggc ggtccgcaag
     2221 ggggccgctg accaggccgc ctcgctgggc tcggccgtca ccctcaagca gacccatgcc
     2281 cccgcggggg tcgtcgctgc gctgcgcgcg gccacgggcc agggcgccta cgtcgtcgcc
     2341 gtgatcgaac gcaaggactc actgaccgag aagaccgcca acgcgctgac gccgtcgaag
     2401 gccttcacga tcctgtccgg caagtcggtc atcaacaaga gcgccgtgct gagcacctac
     2461 gagttcgtgg tcttccacat ccccgccagc ggcaaggcca ctgtcgtcgc cgcggcggag
     2521 caactgtatg ccgcgtccgg cacctgagcg atcggcgaga atgacctcat gagctcgtct
     2581 cccttcactg ccgctgccct gcgcggggcc gtcgacctgt ccgccttcaa acgcccggcc
     2641 aacggcccgg gagcccgccc gggtggggca gccggagcag gttcggccgg ttcggccggg
     2701 gctgctggcg ctgccggggg ttctggcgcg cctggcgcgt cggccagctc gggcggcgac
     2761 ctggtcgtct cggtcaacga tgcgaccttc caggacatcg cgacgaccag ccttcgggtg
     2821 ccgatcgtcg tcgtgctctg gtctgcgcgg ctggctcaga cagcctcctt tgtcggcgtc
     2881 atggccgacg tcgcccgcga caacgggggc cgcttccagg tggcgtcggt cgacgtggac
     2941 agcagcccgg ggctgctgcg ggccttccag atccagtcgg tgccggccac catcggcctc
     3001 gtccaagggc agccggtgcc gctgttcgtc ggagcgctac cgccgtcgga gatccagccg
     3061 tggatcgacg agctgctcaa gttggccgtg cagatgggcg tcacgggtcg ggtctcggtc
     3121 ggggacgacg cgccggcagc cgccgagggc gccgacggcg tcgacggtgc cgaggctgcc
     3181 gaggacgagt tgccgcccct gatcgcagcg gcctacgacg cgatcgagcg tggcgacctg
     3241 gacgcggcga tcgcggcata cgaggaagcc ctcgtgcaga accccgccga cacggaggcc
     3301 gcgctgggcc tcggtcaggt ccggctgctc aagcgcaccg agggcgtcga cgcggttgcg
     3361 gcccgggcgg cggccgcggc cgacccgctc gacgtggcgg cgcagacggt cgtcgccgac
     3421 ctcgatgtcc tcggggggca cgtgcctgac gcgttcgccc ggttggttga cacggttcgg
     3481 gcgacggcgg gcgacgaccg caacgcggcg cgccagcacc tgctcgggct cttcgaggtt
     3541 gttggggcga gtgacgagcg ggtcgtcaag gcccgacgcg cgttgatgag cgcgctgttc
     3601 tgacgcttgg cgtgcgagtc ggctgacggc gcggctggcg ggcaggcggg ttgacgggtt
     3661 gaccgccggt ggtgcgagtc ggtcagtcgt cggcgaccca ccagcggcgc ggttcgccgc
     3721 gcagccggcc ctggatccac cagatcgcct cgatggcggc cgcccctgcc agcccgaccg
     3781 tcagccccgt ccgcatcatc gcggcgttcc cggcgtcgag caggaacagc gactgcgtga
     3841 acggcacgag gaagatcgcc acgtaggccg ccaccgacag caccagcagg gccaccttcc
     3901 accactcgta gggccgggcg acgaccgaca gcacccaggt cgccacgatg atgaggcagg
     3961 ccagggacgc cgtcgaggcc tgggtgcgaa gggcctcgcc ggacccgacc ggcggtcggc
     4021 acagcaggta ggtcgtgaag gtgcagatgg ccacgacgac gccggaggga agggccagtt
     4081 gcaggacccg tcgggcgaac cccggccggg cccgctcgtg gttgggcgcc agcgacagca
     4141 ggaacgccgg cactccgatg gtgaaccacc cggtgatcgt cacgtggatg ggctgcaggg
     4201 ggaacggcag ttgccagatc ccgacgagca gcgcgatcag caccgagtag acggtcttgg
     4261 tgaggaacag gttggcgacc cgttcgatgt tgccgatgac ccggcgcccc tcggcgacga
     4321 cgtaggggag ggtcgcaaac ttgttgtcca gcaacacgat ctgcgcgacg gcccgagtcg
     4381 caggcgatcc cgcgcccatc gcgaccccga tgtccgcctt cttcagggcg aggacgtcgt
     4441 tgaccccgtc accggtcatc gcgaccgtgt gtccacggct cttgagcgcc aggaccatcg
     4501 cgcgcttctg atcgggggtg acccgtccga acacggtatg ccgtgccacg gcgtcgacga
     4561 aggcctccga caggtgcgtg atctggtcgt ggtggccgcc gacgaacccg agcgccgccg
     4621 gctggcccgc ctgggtgtcg gcgtcgtcgg ggtcgacctg tgacagggtc cgggcgtcga
     4681 tcggatcgct gccaccgacg acgccgagca gcgtcgtcac cgcgccgacg gatcgagcgt
     4741 tgtcacccga gatcaccttg acctgcacgt gctgctcggc gaagaactcc agggtgcttc
     4801 gggcgtcggg ccggacccgc tgctcgagga tgacgagcgc cgcaggggtc acggtgccgg
     4861 gtgccgcggg gtcggtgacc ggcagcgacc cctcggccag cagcagcacc cgcagcccgc
     4921 tcgcgccgat ctcctcggcg gcctcgcgag ccgctgaccc ttccggtgcc aggacgtcag
     4981 gggcaccgag cacccaggtg ccctgcccgt cgaacgtcga ccccgaccat ttgcgggccg
     5041 aggtgaaggg ggcgtggtgg gtctcggtcc agatgaccga cggcgcggcg atggcctcgg
     5101 cgatggcggc caggctcgcg ttcgggttgg gctccgtggc tgtgagttgg gcgaggatct
     5161 cggtgacctc ggctggggcc gggccggacg gcataccgga gccgtcgaaa ccgcgcacct
     5221 cggacaggcg catccccgac tcggtgaggg tcccggtctt gtccgcgcag acgacgtcga
     5281 cgcgtgccag gccctcgatg gccggcaact cttggacgag gcactggcgc tgcccgagcc
     5341 ggatgacccc gaccgcgaag gcaatcgacg tcatgagcac gaggccttcg gggaccatcg
     5401 ggaccagcgc ggcgaccatg cccgtcaggc cctcgcgcca cggttgcccg aggccgaaca
     5461 actgggtcca gatcgtcagc aggccgaccg ggacgagcaa ccacgagacg acgaggagga
     5521 tccggttgat cccgtcgcgc aactcggacc cgacgagggt gaacttggcg gcctcctcga
     5581 cgatccgcgc ggcatacgcc tcgcggccga ccttcgtcgc gcggtaggcg ccggaaccgg
     5641 cgacgacgaa actgcccgag agcacctgat cgccgggccc cttgacgacc gggtcggcct
     5701 cgccagtgag cagcgactcg tcgacctcca ggctggccgc ttcgagcacg tcgccatcga
     5761 cgatgagttg gtcgccgggg ccgagctcga tgacgtcgtc ggcgacgatg tcggtgcgag
     5821 tgaccgggcg gctcaccccg tcgcgccgga cggtcgggcg ggcctcgccg atgaccgcga
     5881 ggttgtccag ggtgcgcttg gcgcgcaact cctggatgat cccgatcgcg ctgttggcga
     5941 tgatgagcag cccgaaggcg gcgttgatga gtttgccggt cgtgaggacg atgaccagca
     6001 ggatgccgag gatggcgttg atccgggtga ggacgttggc ccggacgatc tcgccagtcg
     6061 agcgtccgct gcgggcgggc atgtcattcg tgcgcccgtc gcgcacccga tccgccactt
     6121 ctgcgctggt caggccggga tgaccaacgt gcgtcgtgac ctggtccatg gttgggatcc
     6181 taggtgagtg gggcggcggc atagttctgc gaccctggtg cgtcgaacct gatgtgacga
     6241 tggatgtggg tgcgcgggcg caggctcgtc ggcgagaggg ggcgggggcg gtggacctcg
     6301 aaggtttcgt caacgcccac tacgcccagt tgctgcggcg ggcctatctg ctgaccggat
     6361 cacgcgcgga cgccgaggac ctggtgcagg aggcgttggc ccggacctgg gaggccgcgc
     6421 ggggacgggc ggtcgagtcg ccgggggcct acgtccaccg gacgatggtc aacctctatg
     6481 tcagtcgctg gcgccggcgc cggttgcgcc gcgaggatcc ggtggccgag atccccgagc
     6541 cggtggctgg cggcgcgcgg cataccgcgg acgcggtggg cgaggtcgac gagcgggatc
     6601 ggctgtggcg ggtgttgggg tcgctgccgg ccaagcaacg tgcggtgctc gtgctgcgct
     6661 attacgagga cctcagcgag ggcgcggccg ccgacctgct cggggtcacc gtcggaaccg
     6721 ttcgcagtca gacctggaag ggcctgcgcc ggctggaggc cgcgctcggc tctcgcgtgg
     6781 aaggagacgg ccgtggctga cctcgaggcg caggtgcggg cgaccctcgc cgaccgagcg
     6841 ggtcggctgg agggaccgct cccgtatgcc gctccgccgg ctctcgtcga cgccgtggcg
     6901 acccgggctg cgcagttgca ccggcggcgc acccttgtgc gggtgggcgg tgcggctgct
     6961 gcggtggccg tcatcgggct cgcggcggcg accctggtcc gtggcccgtc agtggctggg
     7021 ccgcttggtc cggccggctc ggtcacggcc acggccacgg ccactcgcac ggtcgactcg
     7081 gccacggcca cggccacggc cacggccacg gccacggcca ctcgcacggt cgactcgggc
     7141 acgtc
//
LOCUS       DAORPW010000116         7142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2697_length_7142_cov_669.505432, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000116 DAORPW010000000
VERSION     DAORPW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2697_length_7142_cov_669.505432"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1679
                     /locus_tag="PLB86_09355"
     CDS             <1..>1679
                     /locus_tag="PLB86_09355"
                     /inference="COORDINATES: protein motif:HMM:NF019927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65380.1"
                     /translation="PRTEAENPDQMADDNTQEYFLSYLQWLDADQAGLPERYRKRLGA
                     ALARYGVAGLSRTKALEAATVWLYRAFARIPEIAPAIVAILNRRLTHHDELADMATLD
                     ARARLDRLVRATEGRQQNVSDLARDIIFHFFEEPEMLAATAQMQDQVRADLAALRDLP
                     SGPDREARIEALVAAPHPVRTDLLDAWLQTDADAAGTAYRDVVLEVYARRFYRIRDLQ
                     DFGTRSVGAFRIAHADYEHAGMPVRLVNSYLPLEQLPPFADALRGYLSDVPAGREVVV
                     DLVVWQNGKLPDADALVEQVHGIIEHCDFGHALHRLDLTVTGQVGDSSGRSTAHMTFR
                     QGADGAFTEDRLYRNLHPMLAKRLDLWRLSNFTLERLPSTEDVYLFFGVAHENPKDRR
                     LFAVAEVRDLVAVRDDQTGAQTYPRLGRIGLQALAAMRTALSHYPPRERPTANRLVLN
                     VRPVWEIPADEWPALAANYLPLAKGAGLEKVVMHLRTPVPAEDGGTTLVEKVMTIDGI
                     GRTGTTIRFGDPGANPIRPLTRYAQKVLTAERFGTPYPYEIIHMLTPAEGEP"
     assembly_gap    1680..1779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1780..3186
                     /locus_tag="PLB86_09360"
     CDS             <1780..3186
                     /locus_tag="PLB86_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010143676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRC65381.1"
                     /translation="AAERKIPVEWYACSSGALIAMDSGTENMDFIALTLRRIIEFTQA
                     GHEINIVVTGINVGGQPYWNAEATMLMHTKGILIMTPASTMVLTGKQALDFSGAVSAE
                     DNFGIGGYDRVMGLNGQGQYWAPSFQDACRLLLEHYNYTYVVPGERFPRRGLTSDPVE
                     RDVRESAHEQVPGTRFTTVAEVFDFATNPDRKQPFDMRSVMRAVSDTDCEPLERWKHW
                     QDGENSIVWDVAVGGIPVCMIGLSSRSLPRKGFVPADGPPAWTSGTLFPQASRKTARA
                     INATTGNRPLVVMANLSGFDGSPESMRKWQLEYGAEIGRAITNFNGPIVFIVVSRYHG
                     GAFVVFSKALTESMEIAAIEGSYASVIGGAPAAATVFARDVKIRTDKDPRVVEALAAA
                     AAAKGPEAGRLRAKAAAVAAAVRSEKLGEVADEFDSIHTIERALAMGSVDRIITAEEL
                     RPYIIGALERGMAKFTTS"
     gene            3186..>3469
                     /locus_tag="PLB86_09365"
     CDS             3186..>3469
                     /locus_tag="PLB86_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRC65382.1"
                     /translation="MVLTRDDVLAARERTKGYLRRTPLLPPSRTEAGVWLKCEFLQHT
                     GVFKARGAFNLLLAARERGELDPQAGVVIASGGNAGLATAYAAGVLRVPAT"
     assembly_gap    3470..3569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3570..3827
                     /locus_tag="PLB86_09370"
     CDS             <3570..3827
                     /locus_tag="PLB86_09370"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760894.1"
                     /note="catalyzes the formation of 2-oxobutanoate from
                     L-threonine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HRC65383.1"
                     /translation="DSLGARRVGEIAFAMATASPPISLLVDDEEILRARDLMWRDYRI
                     AAEAGAATAYAALTGGAYRPEPSERVALIVCGANTDPATLR"
     gene            3885..6416
                     /locus_tag="PLB86_09375"
     CDS             3885..6416
                     /locus_tag="PLB86_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRC65384.1"
                     /translation="MPHTASDGMVHVRGAREHNLKNVDVDIPRDALVAFTGVSGSGKS
                     SLAFGTLYAEAQRRYLESVSPYARRLFHQMAVPEVDEIDGLPPAVALQQQRGSSTTRS
                     SVGSVTTLSNLLRMLYSRAGDYPPGQPLLYAESFSPNTPQGACPTCHGLGRVYEVTEK
                     SLVPDDSLTIRERAIAAWPTAWGGQNLRDILVSMGIDVDRPWRDLPQDQREWILFTDE
                     QPVVPVYAGLTPAETRRARKHKEEPSYQGTFSSARRHVFTSFANTESPKMKKRALHFM
                     LSDTCPLCHGKRLRPESLSVTFAGLDLADLSRLPLEELARTIEPYADGSSPRLAEVAA
                     EHPEQALVIGRITGDLRARLSTLLQLGLGYLNLERSTPTLSPGELQRLRLATQVRSNL
                     FGVVYVLDEPSAGMHPADTEALLTALDRLKAAGNSLFVVEHDLDVIRRADWIVDVGPA
                     AGEHGGTILYSGPPEGLRDVASSQTRHFLFESDEARAGRAPREPEDWLRLRGVTRNNV
                     HDLEIAFPKGVFTTVTGVSGSGKSSVVSQALVELVAQALGSDAPAEDAEGDELEIAPV
                     LTLGGEIVSGMEGIKRLVRVDQKPIGRTPRSNLATYTGVFDHIRRLFAATPAARERHY
                     GAGRFSFNVPKGRCPNCNGEGFVMVELLFLPSVYAPCPVCKGTRYNPKTLEITYRGKS
                     IADVLGMTVDGACDFFDDEPAVRRSLTVLCDVGLQYLRLGQPATEFSGGEAQRIKLAT
                     ELQRPQRGDSLYILDEPTTGLHPADVVRLTRQLDSLVASGNTVIAVEHDMSMVAASDW
                     VIDIGPGAGAKGGQIVASGPPREVAQGPGRTAPYLASYLGSPSAG"
     gene            complement(6429..>7142)
                     /locus_tag="PLB86_09380"
     CDS             complement(6429..>7142)
                     /locus_tag="PLB86_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRC65385.1"
                     /translation="WLSAQEAARAATMRFTKRRTEYRLRRLAGKRAVASVLGRPVNPT
                     SLAGIEVLNRRGGAPYVVVDGEHAQVDVSLTDRAGWAVCLVGPEGELTGGTLGVDLEA
                     VEPRSRGFVTDYLTAYEQEYVRSQGELGSEGWDVAANLLWSAKESALKVLRVGLRADT
                     RSVEVTLGDYVRPDGWSPLVVHAGPSERFPGWWRRDGVFLLTLASREVTDPPALLAGG
                     DDLATGEPTHSWMANPLAD"
BASE COUNT         1068 a         2534 c         2281 g         1059 t
ORIGIN      
        1 ccgcgcaccg aggccgagaa ccccgaccag atggccgatg acaacacgca ggagtacttc
       61 ctctcctacc tgcagtggct cgatgccgac caggccgggc tgcctgagcg ctaccgcaag
      121 cgcctcggcg ctgcgctggc ccgctacggc gtcgccggac tgtcgcggac caaggccctc
      181 gaagcggcca ccgtctggct ctaccgcgcc ttcgcccgga tcccggagat cgctccggcc
      241 atcgtggcga tcctcaaccg gcgcctcacc caccacgacg agttggccga catggccacc
      301 ctcgacgcgc gggcccgcct cgaccggctc gtgcgcgcca ccgaggggcg ccagcagaac
      361 gtctccgacc tggcccgcga catcatcttc cacttcttcg aagagcccga gatgctcgcg
      421 gcgacggccc agatgcagga ccaggtgcgg gccgatctcg ccgcgctgcg cgacctgccg
      481 tccgggccgg atcgcgaagc acgcatcgag gcactcgtcg cggcccccca cccggtccgc
      541 accgacctgc tcgacgcgtg gttgcagacc gacgcggacg cggcgggcac ggcataccgg
      601 gatgtcgttc tggaggtcta cgcgcggcgc ttctaccgca tccgcgacct gcaggacttc
      661 ggcacccgct ccgtcggcgc cttccgcatc gcgcacgcgg actacgagca cgccggtatg
      721 ccggtccgcc tggtcaactc ctacctcccc ctggagcagt tgccaccgtt cgccgacgcg
      781 ctgcgcggct acctgagcga cgtgccggcg ggccgggagg tcgtcgtcga cctcgtcgtc
      841 tggcagaacg gcaagctccc tgacgccgac gcactcgtcg agcaggtcca cggcatcatc
      901 gagcactgcg acttcggcca cgccctgcat cgcctcgacc tcaccgtgac cggccaggtc
      961 ggcgattcga gcgggcggtc gacggcacac atgaccttcc gtcagggcgc cgacggtgcc
     1021 ttcaccgagg accggctcta tcgcaacctg cacccgatgc tcgccaagcg gctggacctc
     1081 tggcggttgt ccaacttcac cctggagcgg ctgccgtcca ccgaagacgt ctacctcttc
     1141 ttcggcgtcg cgcacgagaa ccccaaggac cggcgcctgt tcgccgtggc tgaggtccgt
     1201 gacctcgtcg ccgtccgcga cgaccagacc ggagcccaga cctacccgcg gctcgggcgg
     1261 atcgggctgc aggccctggc cgcgatgcgg accgcgttgt cgcactaccc gccccgcgag
     1321 cgcccgacgg ccaaccggtt ggtgctcaac gtgcgaccgg tctgggagat cccggccgac
     1381 gagtggcccg cgctggcggc caactacctg ccgctggcca agggtgccgg gctggagaag
     1441 gtcgtcatgc acctgcggac gccggtgcct gccgaggacg gcggcaccac cctggtcgag
     1501 aaggtcatga ccatcgacgg catcggccgc acgggcacga ccatccgctt cggcgacccg
     1561 ggggccaacc cgattcggcc gctgacgagg tatgcccaga aggtgctcac cgcggagcgc
     1621 ttcggcacgc cctaccccta cgagatcatc cacatgctca cgccggccga gggcgagccn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cggccgagcg gaagatcccc
     1801 gtcgagtggt atgcctgctc gtccggcgcc ctcatcgcga tggactcggg caccgagaac
     1861 atggacttca tcgcgctcac cctgcgacgg atcattgagt tcacccaggc cggtcacgag
     1921 atcaacatcg tcgtcaccgg gatcaacgtc ggcggccagc cctactggaa cgccgaagcg
     1981 acgatgctca tgcacaccaa gggcatcctc atcatgaccc cggccagcac gatggtcctc
     2041 accggcaagc aggccctgga cttctccggg gcggtctcgg ccgaggacaa cttcggtatc
     2101 ggtggctacg accgggtcat ggggctcaac ggccagggcc agtactgggc gccgagcttc
     2161 caggacgcct gccgtctgct gctggagcac tacaactaca cctatgtcgt gcccggggag
     2221 cgtttcccgc ggcgcggcct gacgagcgac ccggtcgagc gggacgtccg cgagtccgcc
     2281 cacgagcagg tgccgggcac ccgcttcacg acggtcgccg aggtcttcga cttcgcgacc
     2341 aaccccgacc gcaagcagcc cttcgacatg cgctcggtca tgcgcgcggt gtccgacacc
     2401 gactgcgagc cgttggagcg gtggaagcac tggcaggacg gcgagaactc catcgtctgg
     2461 gacgtggccg tgggcggcat accggtctgc atgatcggtc tgtcctcccg ttcgctgccg
     2521 cgtaagggct tcgtccccgc cgacgggccg cccgcctgga cctcgggcac gttgttcccg
     2581 caggcgtccc gcaagacggc gcgtgccatc aacgcgacga cgggcaaccg cccgctggtc
     2641 gtcatggcca acctgtccgg cttcgatggc tcgccggagt cgatgcgcaa gtggcagttg
     2701 gagtacggcg ccgagatcgg ccgggccatc accaacttca acggcccgat cgtcttcatc
     2761 gtcgtctccc gctaccacgg cggcgccttc gtcgtgttct ccaaggccct caccgagtcg
     2821 atggagatcg ccgcgatcga ggggtcctac gcctcggtca tcggcggcgc ccccgcggcc
     2881 gcaacggtgt tcgcccgcga cgtcaagatc cgcaccgaca aggacccgcg ggtcgtcgaa
     2941 gccttggctg ccgccgcggc ggccaagggt ccggaggccg gtcggttgcg ggccaaggct
     3001 gctgcggttg cagcggccgt gcgctcagag aagctcggtg aggtggccga cgagttcgac
     3061 tcgatccaca ccatcgagcg ggccctggcg atggggtcgg tcgaccggat catcacggcg
     3121 gaggagttgc ggccctacat catcggcgca ctcgagcgcg ggatggccaa gttcacgacg
     3181 agctgatggt cctcaccagg gacgacgttc tcgccgcccg cgagcgaacg aagggttacc
     3241 ttcgacgcac cccgctcctg cctccgtcac gcacggaggc aggggtgtgg ctcaagtgcg
     3301 agttcctcca gcacacgggt gtgttcaagg cgcgtggagc cttcaacctg ctgctggccg
     3361 cgcgcgagcg gggcgagctc gacccacagg cgggtgtcgt catcgcctcc ggtggcaacg
     3421 ccggtttggc gacggcgtat gccgcgggcg tcctccgcgt cccggcgacn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng actcgctggg tgcccggcgg gtgggtgaga
     3601 tcgcgttcgc catggcgacc gcgtcgccgc ccatcagcct cctggtcgac gatgaggaga
     3661 tcctgcgggc tcgcgacctg atgtggcggg actaccgcat cgccgcggaa gccggcgccg
     3721 cgacggcata cgccgcgttg actggcggtg cttaccgccc ggagccgagc gagcgcgtcg
     3781 ccctcatcgt ctgcggcgcc aacaccgacc cggccacgct gcgctgaccg caccggtccg
     3841 gcgttcaccc tgcttcgggt cggtggcagg aatacagtcg tcgcatgccc cacacggcct
     3901 ccgacgggat ggtgcacgtt cgtggagccc gtgagcacaa cctgaagaac gtcgacgtcg
     3961 acatccctcg cgatgcgctg gtggcgttca caggcgtctc cggctccggg aagtcctcgc
     4021 tcgcgttcgg cacgttgtat gccgaggcgc agcgccgcta cctcgaatcc gtctccccct
     4081 atgcgcgccg cctcttccac cagatggcgg tgccggaggt ggacgagatc gacggcctgc
     4141 caccggccgt tgccctgcaa cagcaacgcg gctcgtccac gacccgctcg tcggtcggca
     4201 gtgtcaccac gctgtcgaac ctcctgcgca tgttgtattc gcgagcaggc gactatccgc
     4261 cgggtcagcc gctgctgtat gccgagtcgt tctcaccgaa caccccgcag ggggcctgcc
     4321 cgacgtgcca cggcctgggg cgggtctacg aggtcaccga gaagtccctc gtcccggatg
     4381 acagcctgac catccgcgag cgggccatcg ccgcctggcc gaccgcctgg ggcggccaga
     4441 acctgcgcga catcctcgtc tcgatgggga tcgacgtgga tcgaccgtgg cgcgacctgc
     4501 cgcaagatca acgcgagtgg atcctcttca cggacgagca gcccgtggtg cccgtgtatg
     4561 ccggcctgac acctgcggag acacgtcgcg cgcgcaagca caaggaggag ccgagttacc
     4621 aggggacgtt ctccagcgcc cgccggcacg tcttcacctc gttcgcgaac accgagagcc
     4681 cgaagatgaa gaagcgggcg ctgcacttca tgctcagcga cacgtgcccg ttgtgccacg
     4741 gcaagcggct gcgtccggaa tccctgtccg tgacgttcgc cggcctggac ctggccgacc
     4801 tgtcgcggct ccccctggag gaactcgccc ggaccatcga gccctacgct gacggctcct
     4861 cgcctcggtt ggcggaggtt gcggccgagc atcccgagca ggcgctggtc atcggccgga
     4921 tcaccggcga tctgcgggcc cgcctgagca ccctgctgca actcgggctc ggctatctca
     4981 acctcgagcg cagcacgccc accctctccc ccggggagtt gcaacgtctg cggctggcga
     5041 cccaggtccg ctccaacctg ttcggcgtcg tctatgtcct ggacgagccc tcggccggga
     5101 tgcacccagc cgacaccgaa gctctgctga ctgcgctcga tcgcctcaag gctgcgggca
     5161 actcgctctt tgtcgtcgag cacgatctgg acgtcatccg tcgcgccgac tggatcgtcg
     5221 acgtcggacc ggcggcgggc gagcacggcg gcaccatcct ctacagcggt ccgcccgaag
     5281 gcctacggga cgtggccagt tcgcagaccc ggcacttcct cttcgagagc gacgaggccc
     5341 gggcggggcg tgcccctcgc gagcccgagg attggctgcg cctgcgaggg gtgacgcgca
     5401 acaacgtcca cgacctggag attgccttcc ccaagggcgt cttcacgacg gtgaccggcg
     5461 tgtccggttc gggcaagtcc agcgtcgtga gccaggcgct cgtggagttg gtcgcgcagg
     5521 ccctgggctc cgacgcccct gccgaggatg ccgaaggcga cgaattggag atcgctcccg
     5581 tgctcacgct gggcggcgag atcgtttccg gcatggaggg catcaagcgg ctggtccgcg
     5641 tcgatcagaa acccattggg cggacgccgc gctccaacct ggcgacctat accggcgtgt
     5701 ttgaccacat tcgtcgcctg ttcgccgcga cgccggccgc gcgggaacgc cactacggcg
     5761 ctggccggtt ctccttcaac gtccccaagg gtcgatgccc gaactgcaac ggcgagggct
     5821 tcgtcatggt cgagttgctg ttcctgccga gcgtctatgc gccgtgcccg gtgtgcaagg
     5881 ggacccgtta caaccccaag accctggaga tcacctaccg cggcaagtca attgccgatg
     5941 tcctcggcat gacggtcgat ggcgcgtgcg acttcttcga tgacgagccg gcagtgcgac
     6001 ggtcgctgac ggtgttgtgc gacgtcggcc tgcaatacct gcggcttggg caacccgcga
     6061 ccgagttctc cggcggggag gcccaacgga tcaagctcgc caccgagttg cagcggccgc
     6121 agcgcggcga ctccctctac atcctcgatg aaccgaccac ggggctgcac ccggcagacg
     6181 tggtgcggct gacccgtcag ctcgattccc tggtggcctc aggcaacacc gtcatcgcgg
     6241 tggagcatga catgagcatg gtggccgcga gcgactgggt gatcgacatc ggtccgggcg
     6301 ccggcgcaaa gggtggtcag atcgttgcgt ccggtccccc gcgagaggtg gcgcagggcc
     6361 cgggccgcac cgcgccctac ctggcgtcct atctgggttc cccatccgcg gggtgacccg
     6421 acgacacctc agtcggcgag cgggttggcc atccacgagt gggtcggctc cccagtggcc
     6481 aggtcgtccc cgcccgcgag cagagcaggc gggtcggtca cctcgcgact ggccagcgtc
     6541 agcaaaaaca ccccgtcgcg ccgccaccag ccggggaacc gctcgctcgg gccggcgtgc
     6601 acgacgaggg ggctccagcc atcggggcgc acatagtcac cgagagtgac ctcgaccgag
     6661 cgagtgtccg cccgcagccc cacgcgcagc accttgagcg ccgactcctt cgccgaccac
     6721 agcaggttgg cggcaacgtc ccagccctcg ctgccgagct caccctggga gcgcacatac
     6781 tcctgctcgt atgccgtgag gtagtcggtc acgaaccctc ggctgcgcgg ctccacggcc
     6841 tccagatcga cccccagcgt gccgcccgtc aattcgccct cgggcccgac cagacacacc
     6901 gcccagcccg cccggtcggt cagcgagaca tccacctggg cgtgctcgcc gtcgacgacg
     6961 acgtatggcg ctcccccacg ccgattgagc acctcgatcc cggccaggct ggtggggttc
     7021 accggacggc ccaggacgga tgcgaccgca cgcttgccgg ccagtcgccg cagccgatac
     7081 tcggtgcgcc gcttcgtgaa gcgcatcgtc gccgcgcggg cggcctcctg cgcggagagc
     7141 ca
//
LOCUS       DAORPW010000117         7136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2703_length_7136_cov_617.062985, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000117 DAORPW010000000
VERSION     DAORPW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2703_length_7136_cov_617.062985"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..441)
                     /locus_tag="PLB86_09385"
     CDS             complement(64..441)
                     /locus_tag="PLB86_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65386.1"
                     /translation="MKLFNEIIVVFHLLGMAAIVGGWLAVRHDLKIVPAILWGARVQL
                     LTGLILAGFASMDKEDPPNNAKLGVKLLVALVVVALSESTWAAQKKAVAGAAQAAVAS
                     KVNLVGGLAVLNVLIAVLWRSYS"
     gene            484..>2795
                     /locus_tag="PLB86_09390"
     CDS             484..>2795
                     /locus_tag="PLB86_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HRC65387.1"
                     /translation="MAPLTHLEVVTAPLVNHAMAHNGIRVVRQITLVSSPDLDGGHTD
                     VVLTARLVDAHSTVLTRPWQHHVDRLDAGGRVRLDNPDITLDPAAIASLDEETGADLI
                     VEATRAGQTFVTSTTPMRVLAARQWLVDPDAPVLSLELLAAFVQPNHPALGPLVARAA
                     AYLEAETTSGSLAVDHVLPGRIDAIVEAVCAAVHASGIYYAQPPASWGYGQKVRTPGD
                     VLEQKVGTCLDTTLLVASALEHLGIHPVLWIVRGHAFLGYWRTTDTGLPDAASLQCAQ
                     AVNAVGLGLMGVVETTMLTQERRPPSDLFRRASQAPRDTYLHGDPAALIGVVDVYLAR
                     LMRVFPMPARRIREDGVVEVIEYRPPTAPTDSVAAQRDSQAPPAQSGIPAPTAATPRR
                     PEPPPRVQAWKNSLLDLTLRNRLLNSARGVTQLPLLMPGEHLGALADLLTAGTPVVVR
                     AADDLSGAVLAQEVRDAYGLPADVLRTMLRDKATVYAAADGETHRLMVARLRYRARTG
                     LQETGANPLALTLGRLDWRLGDRELTAPLLLAPVELKGIVQPFKIVPDETAGLTLNLS
                     LMEKLRIEFGFVVPGLDELPIRPAGVRGEGGVDVDEVVRRLRQALLDAGLPFTVETEA
                     RLAIVGFTGYLLWRDLNEHWERFLTQPVARHLALTPTDRFTDPAAAEVDTSLATLDGV
                     VADVPIPADGSQAEAIAFARGGRTFVLEGPPGTGKSQTITNILADQVARGRRVLFVAE
                     KGAALDVVRRRLAEVGLAPFALDLHDEHATP"
     assembly_gap    2796..2895
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2896..>4283
                     /locus_tag="PLB86_09395"
     CDS             <2896..>4283
                     /locus_tag="PLB86_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65388.1"
                     /translation="FSLVEDADLAVAELERRLAGCSEPVRAALATAIIPDDLERIGWL
                     LTNHAVSPEVLDETRSRRWREARDELEARQTHLDTLAAPVLAVLTPEALSVPVEPIRQ
                     AVREAASSFFIGRKGRLLHAAAPLLVHALPGADIPPKTLPTLVEQVAAVAGETASLAH
                     AWHSLPGLGVVPPDANLLAPQSRSALTDALTAIDRDFAILDRLMSPTANAVREARDNG
                     EVLLDEAYAALRAASSCVAAVMAATGTPVGTVNGLHRLWAEHNVRGVLKAWQAGNRAR
                     LADRGAGATLRQWIEAVTALEPLDRSGLSMAQEAILSGEVPASGAVAALDRGLAGAAL
                     EERLGTRAMAGFDGEAHDRIVDRFVGASDALRGTLRGILPSRVVQERPFKPGATFGTV
                     AALEREVGRTRGGLSVRRLVQTYGEVVGELTPCVLVSPDSLARFIPPGSIDFDLVVFD
                     EASQITVPDAIGA"
     assembly_gap    4284..4383
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4384..>5619
                     /locus_tag="PLB86_09400"
     CDS             <4384..>5619
                     /locus_tag="PLB86_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRC65389.1"
                     /translation="GEPEATAADFRIVPDEESILSEMVHAGVDRLWLSWHYRSQDESL
                     IAFSNGHYYDNRLSSFPAYPGEVTDTGLSFTRVDGTFYRTSSRAARGPGGPGGASPGG
                     PLRTNPVEAAAVVEEVRRRWGQGERSIGVVTFNLQQRTLIEKLLWDLGDETIADSLAS
                     GKDGLFVKNLENVQGDERDVIIFSTGFAKTASGVLPLNFGPLNRTGGERRLNVAITRA
                     RRRVMVFSSFEPEDLRVEQTSSVGVQHLRAYLEVAKYGVGHAPTGGGGLVGTAAGLLS
                     DGDRSAPRGRIDAVDRHKDDVAAALRAEGLSVTVGVGLSDFKVDLAVGPPGKPPVLAI
                     LLDGPEWAERRTTNDRDGLPTTVLQHIMGWPMVMRIWLPSWLFSRDDVVATVCERAEI
                     ASRLPRHVGERRVSTGYAAA"
     assembly_gap    5620..5719
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5720..5926
                     /locus_tag="PLB86_09405"
     CDS             <5720..5926
                     /locus_tag="PLB86_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65390.1"
                     /translation="RELANALVAIARSAMGIDLDELLKQTLTVFNGTRLTEVPRRRLM
                     AALQVAQDRGQLNVSGDIVTATDG"
     gene            5933..>7136
                     /locus_tag="PLB86_09410"
     CDS             5933..>7136
                     /locus_tag="PLB86_09410"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HRC65391.1"
                     /translation="MAADPLTVARPFQRDLLIVIGLAASPLVALGCSRFAYALLLPAM
                     RSDLGWSFTTAGLMNTVNAVGYLVGALGTAWAAAKLGQRPAFVGSLVITVLALAATAA
                     SSSLEVLLAVRTILGIAGAGAFVLGGAITSTISRRHTPHRAAVLIGTYFAGGGVGIVA
                     SGLLVPAVFERLGPAGWPTGWLVLAVLAAVGTVAATIAAYAAPTTPAAAPGSRSFWVS
                     GLGRLASGYLLFGAGYIGYMTFIIAMLTQVGLSPKEIAAFWVALGVAGAVSGQVWARL
                     LRGARGGSAAAVLFVLLAVATGIPALTSATPALYASGVLFGLCFLSLVGAVTAAGRDA
                     VEPADTTAALGMLTTVFALGQVIGPWFTGLLADRTGGQQWGLGVSAVVLLIGAVLCRL
                     QPPRRIGHD"
BASE COUNT          950 a         2578 c         2244 g         1064 t
ORIGIN      
        1 gccgggtcga ggtattcggc catcgtctgg tgacggtgga atacctcggc ccggcaagct
       61 ggctcagctg taggagcgcc agagcacggc gatcaggacg ttgaggaccg ccaaaccccc
      121 gacgaggttg accttcgagg ccacggcggc ctgggccgcg cccgcgacag ccttcttctg
      181 cgccgcccag gtcgactccg acagcgccac gacaaccaac gcgaccagga gcttgacccc
      241 cagcttggcg ttgttgggcg ggtcctcctt atccatggaa gcaaaccccg cgaggatcag
      301 cccggtcaac aactgaaccc gcgcgcccca caggatggcg ggcacgatct tgaggtcgtg
      361 ccggacggcc agccacccac cgacgatggc ggccatgccc aacaggtgga agacgacgat
      421 gatctcgttg aacagcttca tggcgtcagg ctatccgcac tccccacggt ctagagtctg
      481 gccatggcgc ccttgaccca ccttgaggtg gtcacagcgc cgctggtcaa ccacgcgatg
      541 gcccacaacg ggatccgcgt cgtgcgccag atcaccctgg tctccagccc cgacctggac
      601 ggtggccaca ccgacgtcgt cctcaccgcc cggctcgttg acgcccacag caccgtgctc
      661 acccggccct ggcaacacca cgtcgaccgc ctcgacgccg gagggagggt gcgcctcgac
      721 aacccggaca tcaccctcga cccggccgcc atcgccagcc tcgacgaaga gaccggcgcc
      781 gacctcatcg tcgaagccac ccgcgccggc cagaccttcg tcacctcgac cacgccgatg
      841 cgggtcctcg cggcccgcca atggctggtc gacccggacg cccctgtcct ctccctcgag
      901 ttgctcgccg ccttcgtcca gcccaaccat cccgcgctag gccccctcgt ggcgcgggcg
      961 gcggcatacc tggaagcgga gacgacctcc ggctcactgg ccgtcgacca cgtcctgcct
     1021 ggccggatcg acgcgatcgt cgaggcggtg tgcgcggccg tccacgcgtc aggcatctac
     1081 tacgcccagc cgccggcctc gtgggggtat ggccagaagg ttcgcacccc cggcgacgtc
     1141 ctggagcaga aggtcggaac ctgcctggac accacgttgc tcgtcgcctc agcgctggag
     1201 cacctcggca tccacccggt cctctggatc gtccgcggcc acgccttcct cggctattgg
     1261 cgcacgaccg acaccgggct gcccgacgcg gccagcctgc agtgcgcgca ggccgtcaac
     1321 gcggtcgggc ttgggctcat gggtgtcgtc gagacgacca tgctcaccca ggagcgacgc
     1381 cctcccagcg acctgttccg acgcgcgtcg caagcgcctc gggacaccta tctgcacggt
     1441 gacccggcgg cgctgatcgg cgtggtcgac gtctacctcg cccgactcat gcgggtgttc
     1501 cccatgcctg cgcgacgcat tcgcgaggac ggtgtcgtcg aggtcatcga ataccgaccc
     1561 ccgaccgcgc ccaccgactc cgttgcggcc caacgcgact cgcaagcccc accggcgcaa
     1621 agcggcatac ccgctcccac cgcggccacc ccgcgccgac ccgagccgcc cccgcgggtg
     1681 caagcgtgga agaactccct gctggacctc accctgcgca accggctgct caactctgcg
     1741 cgcggcgtca cccaactgcc cctgctcatg cccggcgagc acctcggcgc cctggccgat
     1801 ctgctcaccg cgggcacccc cgtggtcgtc cgcgcggctg acgacctctc tggtgcggtg
     1861 ctcgcccaag aggtccgcga cgcctacgga ctgcccgccg acgtcctgcg caccatgctg
     1921 cgcgacaagg ccacggtgta tgccgccgcc gacggcgaga cccatcggct catggtggcg
     1981 aggcttcgtt accgcgcccg gaccgggctg caggagaccg gagccaaccc gctggcgctc
     2041 accctcgggc ggctggactg gcgactcggc gaccgcgagt tgaccgcgcc gctgctcctg
     2101 gcacccgtcg agctcaaggg cattgtccaa cccttcaaga tcgtccccga cgagacggcc
     2161 ggcctcacgc tcaacctctc gctgatggag aagctgcgga tcgaattcgg tttcgtggtg
     2221 ccgggattgg acgagctccc catccgtcca gcgggagtcc gcggcgaggg cggggtcgat
     2281 gtcgacgagg tcgtgcggcg cctgcgccaa gccctgctcg atgcgggcct gcccttcacc
     2341 gtcgagaccg aggcgcgcct ggccatcgtc ggtttcaccg gctacctgct ctggcgagac
     2401 ctcaatgagc actgggaacg gttcctcacc cagccggttg cccgccacct cgccctgacc
     2461 ccaaccgacc ggttcaccga tcccgctgcg gcagaggtgg atacgagcct ggctaccctc
     2521 gacggcgtgg tcgccgacgt gcccatcccc gcggacggct cgcaggccga ggcgattgcc
     2581 ttcgcccgcg gtgggcgcac cttcgtcctg gaaggcccgc cgggcaccgg caagtcccag
     2641 accatcacca acatcctcgc cgaccaggtc gcgcgggggc gccgggtgct gttcgtcgcc
     2701 gagaagggcg cggcgctcga cgtcgtgcgc cgccgcttgg cggaggtcgg cctcgccccg
     2761 ttcgcgctcg acctgcacga cgagcacgcg acgccnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnttcag cctcgtcgag gacgccgatc ttgcggtcgc cgagctcgag
     2941 cgccggctgg ccggctgcag cgagcccgtc cgggccgccc tggccacggc catcatccct
     3001 gacgacctgg agcgcatcgg ctggctcctg accaaccacg ccgtctcccc ggaggtcctc
     3061 gacgagaccc gctcccgacg ctggcgcgag gcccgagacg aactcgaggc ccgccagacc
     3121 cacctcgaca cgctggccgc tcccgtgctc gccgtcctga ctcccgaggc gctgagcgtc
     3181 cccgtggaac ccatccgcca ggcggtccga gaggcggcct cgtcgttctt catcggccgc
     3241 aagggccgcc tgctgcatgc cgccgccccc ctgctcgtgc atgcccttcc aggtgccgac
     3301 atcccgccca agaccctgcc tacgcttgtt gagcaggtcg ctgcggtggc cggcgagacg
     3361 gcatccctcg cgcacgcctg gcactctctc ccggggctcg gcgtagtccc acccgacgcg
     3421 aatctccttg ccccccaaag tcgtagcgcg ctcaccgatg cgctcaccgc catcgaccga
     3481 gacttcgcca tcctggaccg gttgatgtcc cccacggcga acgcggttcg cgaggcgcgc
     3541 gacaacgggg aggtcctgct cgatgaggcg tatgccgccc tgcgcgccgc ctcgtcgtgc
     3601 gttgccgccg tcatggccgc caccgggacc cctgtgggga ccgtcaatgg actgcaccgg
     3661 ctgtgggccg agcacaatgt ccggggcgtg ctgaaggcct ggcaggccgg caaccgggcg
     3721 cggctcgccg acagaggcgc aggtgccaca ttgcgccaat ggatcgaggc agtcacagcc
     3781 ctcgaacccc tggaccggtc cggtctgtcc atggcgcagg aggcaatcct gtcgggcgag
     3841 gtgcccgcgt ccggggccgt cgcggcactc gaccggggct tggcgggtgc cgccttggag
     3901 gaacgactgg gcacgagagc gatggctgga ttcgacgggg aggcgcacga ccggatcgtc
     3961 gaccgcttcg tcggggcatc ggacgcgctg cgggggaccc tgcgcggcat actccccagc
     4021 cgggtggtgc aggagcggcc gttcaagccg ggagcgacgt tcgggaccgt ggccgccctg
     4081 gaacgcgagg tgggtcgcac gcgaggaggg ctgagcgttc ggcggttggt gcagacctat
     4141 ggcgaggtcg tcggggagct caccccatgc gtgctcgtca gccccgactc cctggcgcgc
     4201 ttcatcccgc cagggtcgat cgacttcgac ctggtcgtgt tcgacgaggc ctcgcagatc
     4261 acggtgccgg atgcgatcgg cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnggcgagc ccgaggcgac ggctgccgac ttccggatcg tgcccgacga ggagtcgatc
     4441 ctctcggaga tggtccacgc aggcgtcgac cggctgtggc tgtcgtggca ctaccgcagc
     4501 caggacgagt cgctcatcgc gttctcgaac ggtcactact acgacaaccg gctcagctct
     4561 ttcccggctt atccgggcga ggtcaccgac acggggttgt cgttcacccg ggtcgacggc
     4621 accttctatc gcacctcgtc gcgcgccgct cgcggcccgg gcggcccggg cggcgcgtcg
     4681 cccggcggcc cgttgcggac caatcccgtc gaggctgctg ccgtggtcga ggaggtgcgc
     4741 cgccggtggg gccagggcga gcgctcgatc ggcgtcgtca ccttcaacct ccaacagcgc
     4801 actctcatcg agaaactgct ctgggacctc ggcgacgaga ccatcgccga cagcctggcc
     4861 agcggcaagg acggcctgtt cgtcaagaac ctcgagaacg tccagggcga cgagcgcgac
     4921 gtcatcatct tctcgaccgg gttcgccaag accgcgtccg gtgtcctacc gctcaacttc
     4981 gggccgctta accgcactgg gggcgagcgg cgcctcaacg tcgccatcac ccgcgcgcgc
     5041 cgtcgggtca tggtgttcag ttccttcgag cccgaagacc tgcgggtcga gcagacctcg
     5101 tcggtcggcg tccaacacct gcgcgcctat ctcgaggtgg ccaaatatgg cgtcggacac
     5161 gcgcccacgg gcggaggcgg gctcgtcggc actgccgccg gcctcctcag cgacggcgac
     5221 cgctcagctc cccgcggacg catcgacgcc gtcgaccggc ataaggacga cgtcgccgcc
     5281 gccctgcggg ccgagggcct gtccgtgacc gtgggcgttg gtctgtccga cttcaaggtc
     5341 gatctcgcgg tcggcccgcc cgggaaacct cccgtgctcg cgatcctgct cgacgggccc
     5401 gagtgggcgg agcgccgaac gaccaacgat cgcgacggcc tccccacaac tgtcctgcag
     5461 cacatcatgg gctggccgat ggtcatgcgg atctggctgc cctcgtggtt gttctctcgg
     5521 gacgatgtgg tcgccaccgt gtgcgagcgg gccgagatcg cctctcgcct gccgcgccac
     5581 gtcggtgagc gccgggtgag cacggggtat gccgccgccn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnc gcgagctcgc caacgccttg gtcgccatcg ctcggtcggc
     5761 gatgggcatc gatctcgacg agttgctcaa gcagaccctg acggtcttca acggcacccg
     5821 gctcacagag gtcccccggc gccgcctgat ggccgccttg caggtcgcac aggaccgtgg
     5881 gcagttgaac gtcagcggcg acatcgtcac agccacggac ggctaggcgg tcatggccgc
     5941 cgacccgctc actgtggcac gccctttcca gcgcgacctg ttgatcgtca tcggcctcgc
     6001 cgcctccccg ctcgtggccc tgggttgctc gcggttcgcc tatgcgttgc tgctgcccgc
     6061 catgcgctcg gacctcgggt ggtcgttcac cacggccggg ctgatgaaca cggtcaacgc
     6121 cgttggttac cttgtgggtg cactcggaac cgcctgggcc gcagcgaaac tcggccaacg
     6181 ccccgccttc gtcggctcgc tcgtcatcac cgtgcttgcc ctcgccgcga ccgccgcgtc
     6241 aagttccctg gaagtcctcc tggcggtccg cacgatcctc ggcatcgcgg gcgcaggcgc
     6301 cttcgtcctc ggtggcgcga tcacctcgac gatcagcagg cggcataccc cgcaccgcgc
     6361 ggccgtgctc atcgggacct acttcgcggg cggcggggtg gggatcgtcg cctccggcct
     6421 gctcgtcccc gcggtgttcg agcggctcgg gccggcggga tggcccaccg ggtggctggt
     6481 tctcgcggtc ctcgcggcgg tcggcacggt ggccgccacc atcgccgcgt atgccgcccc
     6541 caccactccc gctgcggccc ccgggtcgcg gagcttctgg gtcagcggcc tgggccgact
     6601 tgctagcggc tacctgttgt tcggtgccgg ctacatcggc tacatgacgt tcatcatcgc
     6661 catgttgacc caggtcgggc tgtctcccaa ggagattgcc gcgttctggg ttgcgctcgg
     6721 cgtggccggg gcggtgagcg ggcaggtctg ggcgcggctg ctgcggggtg ctcgcggagg
     6781 ctccgcggcg gcagtcctct tcgtgctgct cgcggtcgcg accggcatac ccgccctcac
     6841 gtccgccacc cccgcgctct atgcgtcggg cgtgctgttc gggttgtgct tcctctcgct
     6901 cgtcggggcc gtcaccgccg caggtcgcga cgccgtcgag ccagccgaca cgaccgccgc
     6961 gctcgggatg ctcaccacgg tgttcgccct gggccaggtc atcgggccct ggttcaccgg
     7021 actgctcgcc gaccgtaccg gcggccaaca atggggcctc ggagtctcgg ccgtcgtgct
     7081 cctcatcggc gccgtcctct gccgcctcca acccccgcgc cggatcggac acgacg
//
LOCUS       DAORPW010000118         7109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2714_length_7109_cov_767.559682, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000118 DAORPW010000000
VERSION     DAORPW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2714_length_7109_cov_767.559682"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PLB86_09415"
     CDS             complement(<1..913)
                     /locus_tag="PLB86_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRC65392.1"
                     /translation="MALNPRTRGPRRTLWTFAAIIIALFVLIFSVNRWGTGQLTPKLG
                     LDLEGGTQMVLEPVLADPNAEVSSGQLEKARDIIAQRVDSNGVAEAEVSTQGGRNIVI
                     SIPGQPDAKTLDQIRKPSQLRFRAVLVTGSGIPATVTTPTSTATGTQTSEPTATTPKP
                     VFTMDPAPTATSPATSGNAVVPPALVSETSPTAPAATSTPTATAAATPTPANASDLAW
                     ITPEIEAEFTALDCTKAGAINDFVDDPAKPLVTCSTDRVEKYILGPVELDGTDIADAT
                     SGYQSGNNGQPTNIVEVRLNFTGEGGQK"
     gene            complement(925..1239)
                     /gene="yajC"
                     /locus_tag="PLB86_09420"
     CDS             complement(925..1239)
                     /gene="yajC"
                     /locus_tag="PLB86_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRC65393.1"
                     /translation="MTSNDLSSLLFFAIPVGFIAYIFLGQRKRVRAMQAMQASIVVGE
                     EVRTTSGLFGRVTALTDSEIHLEVAPGVVVRFDRRAVDARTSGAAPSSSNGPADAPAQ
                     GE"
     gene            complement(1365..2495)
                     /gene="ruvB"
                     /locus_tag="PLB86_09425"
     CDS             complement(1365..2495)
                     /gene="ruvB"
                     /locus_tag="PLB86_09425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRC65394.1"
                     /translation="MSTSDDPTETDGPGGPTWADGSWEATARVVDPSGLDRERQVEAA
                     LRPRRLAEFPGQTRVRDQLALVLEAARRRGTPPDHVLLSGPPGLGKTTLAMIIAAELE
                     QPIRVTSGPAIQHAGDLASILSSLAEGEVLFLDEIHRMSRAAEEMLYLAMEDFRVDIV
                     VGKGPGATAIPLELPPFTVVGATTRSGLLPAPLRDRFGFTGHLDFYETADLVTILRRS
                     ARLLDVRAEDAGIEEIAKRSRGTPRIANRLLRRVRDWAQIHGQDVVDLDAAHEALALF
                     DVDVRGLDRLDRAVLDALCRRFGGGPVGVANLAVAVGEEADTVETVAEPFLVREGLIV
                     RTSRGRAATALAWEHLGLAPPSQAAAYQASLLTPDDVGRFGT"
     gene            complement(2492..3091)
                     /gene="ruvA"
                     /locus_tag="PLB86_09430"
     CDS             complement(2492..3091)
                     /gene="ruvA"
                     /locus_tag="PLB86_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRC65395.1"
                     /translation="MIASVRGEVTHVGLDNVVVEVGGVGVLVHVTPATAATLRTGSTA
                     YLATSLVVREDSLTLYGFADADGRSVFETVQTVTGVGPRLALAMLAVHSPDGIRAAVA
                     SGDLTALMKVPGIGRKGAERLVLELRDKMGMPSGPVGSPTAAAAGPDWAHQVREALVG
                     LGWSAKQAEDTVEAITPEAGERPDVSGLLRTALQRLART"
     gene            complement(3088..3639)
                     /gene="ruvC"
                     /locus_tag="PLB86_09435"
     CDS             complement(3088..3639)
                     /gene="ruvC"
                     /locus_tag="PLB86_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRC65396.1"
                     /translation="MRVLGVDPGLTRCGVGVVEGDLGRRLAMVDVTVVRSDSKTPTPE
                     RLLALDLALTELVGRHRPDAIAVERVFADVNVLSVMGTAQASAVAMLVAARHGIPVYL
                     HTPTEVKAAVTGSGRAEKAQVTAMVTRLLRLPTAPTPADAADALALAICHLWRGAATN
                     RIEQAAATVRAQQAAALAGRTGR"
     gene            3770..4171
                     /locus_tag="PLB86_09440"
     CDS             3770..4171
                     /locus_tag="PLB86_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRC65397.1"
                     /translation="MSTKSTGGRQRASSGSPFEATIGFSRGLRDGEVIAIAGTAPVWP
                     DGSVDPDPLVQARRAWAIALTALSQLGGRPQDVVRTRTYLTTTEVAEVAAEAHGEIFS
                     DVRPASTMVVVAALLDPRWVIEVELDAIVRG"
     gene            complement(4217..4972)
                     /locus_tag="PLB86_09445"
     CDS             complement(4217..4972)
                     /locus_tag="PLB86_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRC65398.1"
                     /translation="MSGHSKWATTKHKKAAIDAKRGKLFAKLIKNIEVAARTGGGDPL
                     GNPTLWDAIYKAKKSSVPNDNIDRAVKRGSGAEAGGAEYKEITYEGYAPNGVALYIQC
                     LTDNRNRAAAEVRTALTRNGGNLADPGSVAYMFTRKGVVIVPKGSHTEDTLLEVVLDV
                     GAEEINDLGEAFEIVSEASDFVEVRKALQAAGIDYDSAEATFIPSVEVPLDLEGARKM
                     MRVIDALEDSDDVQNVYANGDISDEVLAQLDEE"
     gene            complement(5026..5631)
                     /gene="pdxT"
                     /locus_tag="PLB86_09450"
     CDS             complement(5026..5631)
                     /gene="pdxT"
                     /locus_tag="PLB86_09450"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HRC65399.1"
                     /translation="MTTAPTIGVLALQGDVREHVHALEAVGATAVPVKLESELHGIQG
                     IVLPGGESTVIDKLLRSFGLFEPLRDRLRGGLPAYGSCAGMILLADRVTDATADQQTL
                     GGIDMTVRRNAFGRQVDSFEQDVAYADWPAPVHAVFIRAPWVEQVGESVEVLARVDGG
                     RADGRIVAVRHGALLATSFHPEVTGDHRVHAQFVDMVRERG"
     gene            complement(5643..6230)
                     /locus_tag="PLB86_09455"
     CDS             complement(5643..6230)
                     /locus_tag="PLB86_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65400.1"
                     /translation="MADHWVSNTLTVVGILAAVAFSVWLAWFGWSRFLPVAAELRQRE
                     RAAQIPPSAERSTVNATFDVLAFNARLPVRRPRHLALADGWLTFTVPGHSRPTLQVAA
                     EHLWMQIDEKDRLLVYGPSIARITISRVADVDGALQSRPIDDSPLVATLAANGVRVPL
                     GSRPRLPQGPKPERANLTALRRRGPSWPWAHRPSR"
     gene            complement(6303..>7109)
                     /gene="pdxS"
                     /locus_tag="PLB86_09460"
     CDS             complement(6303..>7109)
                     /gene="pdxS"
                     /locus_tag="PLB86_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRC65401.1"
                     /translation="TPDQAKIAEDAGAVAVMALERVPADIRAQGGVSRMSDPDMIDGI
                     IAAVSIPVMAKARIGHFVEAQVLQSLGVDYIDESEVLTPADYTNHIDKWAFNVPFVCG
                     ATNLGEALRRITEGAAMIRSKGEAGTGDVSNATTHMRQIRGEINRLRSMTPDELYVAA
                     KELQAPYDLVKEVALAGKLPVVLFTAGGIATPADAAMMMQLGAEGVFVGSGIFKSGNP
                     AQRAEAIVKATTFYNDADVIAKVSRGLGEAMVGINVEELPQSHRLAERGW"
BASE COUNT         1079 a         2438 c         2501 g         1091 t
ORIGIN      
        1 acttctggcc gccctcaccg gtgaagttca accgaacctc gacgatgttg gtcggttgac
       61 cgttgttgcc cgactggtag cccgacgtcg cgtcggcgat gtcggtgccg tcgagctcaa
      121 cgggaccgag gatgtacttc tcgacccggt ccgtcgagca ggtgaccagc ggcttggcgg
      181 ggtcgtcgac gaagtcgttg atcgcgccgg ccttggtgca gtcgagggcc gtgaactccg
      241 cctcgatctc gggcgtgatc caggccaggt cggaggcgtt ggccggcgtc ggggtggccg
      301 ccgcagtggc cgtgggcgtt gacgtggctg ccggcgccgt cggggacgtc tcggagacca
      361 acgccggcgg aacgacggcg ttgccactcg tggccgggct cgtcgccgtg ggggccgggt
      421 ccatggtgaa gaccggcttg ggcgtcgtcg cggtcggctc gctcgtctgc gtcccggtgg
      481 ccgtcgaagt gggcgtcgtg acggtggcgg gaatgccgga cccggtcaca agaacagcgc
      541 ggaagcgcaa ctgcgacggc ttgcggatct ggtcgagcgt cttggcgtcg ggctgaccgg
      601 ggatcgagat gacgatgttc cgcccgccct gggtcgacac ttcggcttcg gcgacgccgt
      661 tggagtcgac ccgctgggcg atgatgtcgc gggccttctc gagctgcccg gagctcacct
      721 cagcattggg gtcggcgagc accggctcaa ggaccatctg ggtgccgccc tcgaggtcga
      781 ggccgagctt gggcgtcaac tgcccggtcc cccagcggtt gaccgagaag atgagcacga
      841 agagggcgat gatgatcgcc gcgaaggtcc acagggtgcg acggggcccg cgcgtccggg
      901 ggttcaatgc catgactggt tccctcactc gccctgggcc ggggcgtcgg ccggcccgtt
      961 actcgacgac ggtgcagcac cggacgtgcg ggcgtccacg gcgcggcggt cgaacctcac
     1021 gacgactccg ggggccacct ccaggtggat ctccgagtcg gtcagcgcgg tgacccggcc
     1081 gaagaggccc gaggtggtcc gcacctcctc accgacaacg atcgatgcct gcatcgcctg
     1141 catggcgcgg acccgcttgc gctggccgag gaagatgtag gcgatgaagc ccacggggat
     1201 cgcaaagaac agcagcgaac tcaggtcgtt gctcgtcatg tctcgtcgtt acctttatca
     1261 ccggtgaagc acgccactgc cgaacaggca gaccagcgtc ccattctggc ctatcaaccg
     1321 gccccaggcg aaccagccgc gccggaaagc acgcggcata cgcctcaggt gccgaaacga
     1381 ccgacgtcgt cgggggtcag caacgacgcc tggtatgccg ccgcttggct cggcggggcg
     1441 aggccgaggt gctcccacgc gagggcggtc gcggctcggc cacgggacgt gcgcacgatg
     1501 agcccttccc gcacgaggaa cggttcggcg accgtctcga cagtgtcggc ctcctccccc
     1561 acggcaacgg caaggttggc cactccgacc ggaccgccgc cgaagcgccg gcacagcgcg
     1621 tcgagcacgg cccggtcgag ccggtccaag ccgcggacgt cgacgtcgaa gagggccagc
     1681 gcctcgtggg ccgcgtcaag gtcgacgacg tcctgaccgt ggatctgcgc ccagtcacgc
     1741 acccggcgta gcaggcggtt ggcgattcga ggtgttccgc gggaccgctt ggcgatctcc
     1801 tcgatcccgg cgtcttcggc acgcacgtcg agcaaccggg ccgaacggcg caggatcgtc
     1861 accagatcgg ccgtctcgta gaagtccagg tgcccggtga agccgaaccg gtcgcgcagc
     1921 ggagccggca gcagcccgga gcgggtcgtc gcgccgacga cggtgaacgg cggcaactcc
     1981 aacgggatgg ccgtcgcccc cggacccttt ccgacgacga tgtcgacccg gaagtcctcc
     2041 atcgccagat agagcatctc ctcggcggcg cgggacatcc ggtggatctc gtcgaggaag
     2101 agcacctcac cctcggccag cgaggagagg atcgaggcca ggtcaccggc gtgttggatg
     2161 gccggccccg acgtcacccg gatcggttgc tcgagctcgg ccgcgatgat catcgcgagg
     2221 gtggtcttgc ccaggccagg aggcccagag agcagcacgt ggtccggtgg cgtgccacgg
     2281 cggcgcgcgg cctcaaggac gagggccaac tggtcgcgca cccgcgtctg cccagggaac
     2341 tcggccaggc gccgggggcg caaggcggcc tcgacctggc gttcacggtc caggccgctg
     2401 gggtcgacga ctcgcgccgt cgcctcccac gacccgtcgg cccaggtcgg gcctccggga
     2461 ccgtccgtct cggtggggtc gtcgctcgtg ctcacgtgcg ggccaaccgt tgcagggcgg
     2521 tgcgcagcag ccccgacacg tcgggccgct caccggcctc gggcgtgatc gcctcgacgg
     2581 tgtcttcggc ctgcttggcc gaccatccga ggccgaccaa ggcctcgcgg acctggtgcg
     2641 cccagtcggg gcccgcagcg gcggcggtcg ggctgccgac cgggccggac ggcataccca
     2701 tcttgtcgcg aagctcgagc accagccgct cggcaccctt gcggccgatg ccgggcacct
     2761 tcatcagcgc ggtgaggtcg cctgaggcga cggccgcacg gatgccgtca gggctatgga
     2821 cggcgagcat cgccagcgcg agtctcggcc ctacaccggt caccgtctgg acggtttcga
     2881 agaccgagcg gccgtctgcg tcggcgaagc catagagcgt cagcgagtcc tcgcgcacga
     2941 cgagcgaggt cgcgaggtat gccgtgctgc cggtccgcag ggtcgccgcg gtggcgggcg
     3001 tgacgtgcac cagcaccccg acgccgccca cctcgacgac aacgttgtcc aacccgacgt
     3061 gggtgacctc gccacggacg gaggcgatca tcggccggtc cttcctgcca gtgcggcagc
     3121 ctgctgggcc cggacggtcg ccgcggcctg ctcgatccgg ttggtcgccg cgccgcgcca
     3181 caggtggcag atcgccaggg cgagggcgtc ggcggcgtcg gccggggtgg gtgcggtcgg
     3241 cagccgaagc agccgggtca ccatggccgt cacctgcgcc ttttcggcgc ggccgctgcc
     3301 ggtgactgcc gccttcacct cggtcggggt gtgcaaatag acgggtatgc cgtgccgcgc
     3361 cgcgacgagc atcgccaccg ccgaggcctg cgcggtcccc atgaccgaca ggacgttgac
     3421 gtcggcgaaa acccgctcga cggcgatggc atccgggcgg tgtcggccga ccaactcggt
     3481 gagggccagg tcgagcgcca acaggcgctc gggcgttggg gtcttggagt cgctgcgcac
     3541 gacggtgaca tcgaccatgg ccagtcggcg gccgaggtcg ccctcgacga caccgacccc
     3601 gcaccgggtc aggcccgggt cgacgcccag gacgcgcacg gcatacctcc catgtctggt
     3661 gcgaacacgt gttcgggagt cacgctaccc cgttggggcc ggtggctcct ttcgcgacgc
     3721 gccgggacgg ggtgcgccga ggcctccgac gtgccggaga atcactgcca tgagcactaa
     3781 gtcgaccggc gggcggcagc gcgcgtcatc cggatcaccg ttcgaggcaa ccatcggttt
     3841 cagccgggga ttgcgcgacg gcgaggtcat cgccatcgcg ggcacggccc ccgtctggcc
     3901 agacggcagc gtcgacccgg acccgctcgt ccaggccaga cgtgcctggg cgatcgcgct
     3961 caccgccctg tcacagctgg ggggccgacc gcaggacgtc gtgcgaaccc gcacctacct
     4021 gacgacgacc gaggtcgccg aggtggcagc agaggcccac ggcgagatct tcagcgacgt
     4081 gcgaccggct tcgacgatgg tcgttgtcgc tgcgctgctg gacccccgct gggtcatcga
     4141 ggtcgagttg gacgccatcg tgcggggtta gtgcccggca tcggtgggtg cgccggcgga
     4201 gggccgagaa ggggctttac tcctcgtcga gctgagcgag gacctcgtcg ctgatgtcgc
     4261 cattggcata gacgttctgg acgtcgtcgg agtcctccaa cgcgtcgatc acccgcatca
     4321 tcttgcgcgc accctcaaga tccagcggca cctcgacgct ggggatgaag gtcgcctcgg
     4381 ccgagtcgta gtcgatgccg gccgcctgca gcgccttgcg gacctcgacg aagtcactgg
     4441 cctccgacac gatctcgaac gcctcaccga ggtcgttgat ctcctcggcg cccacgtcga
     4501 gcaccacctc aaggagcgtg tcctccgtgt ggctgccctt ggggacaatg acgacgccct
     4561 tgcgggtgaa catgtaggcc accgagccgg gatcggcgag gttgccgccg ttgcgcgtga
     4621 gagcggtgcg cacctcggcg gcggcacggt tgcggttgtc ggtgaggcac tggatgtaga
     4681 gggcaacacc gttgggggcg tagccctcgt aggtgatctc cttgtactcc gctcccccgg
     4741 cctcagcacc cgaaccgcgc ttgaccgcac ggtcgatgtt gtcgttgggg accgacgact
     4801 tcttcgcctt gtagatggcg tcccagaggg tggggttgcc gagggggtca ccgccgccgg
     4861 tgcgggccgc gacctcgatg ttcttgatga gcttggcgaa caacttgccg cgcttggcgt
     4921 cgattgccgc cttcttgtgc ttcgtggtcg cccacttgga gtgaccgctc atgaccccat
     4981 cctcggattc tcgtgcgtcg gaacctgtcg atcctatccg ccgactcagc cgcgctcgcg
     5041 caccatgtcg acgaactggg cgtggactcg gtggtcgccg gtcacctcag ggtggaacga
     5101 ggtcgccaga agcgcgccgt ggcgcacggc gacgatccgc ccgtcggcgc gcccgccatc
     5161 gacccgggcc aacacctcca ccgactcccc gacctgctcg acccaggggg cccggatgaa
     5221 gacggcgtgc acgggagccg gccaatcggc atacgcgacg tcctgctcga acgagtcgac
     5281 ctgacggccg aatgcgttgc gccgcaccgt catgtcgatc ccgccgaggg tctgctgatc
     5341 ggccgtcgcg tcggtgaccc ggtcggcgag caggatcatc ccggcacagg agccgtaggc
     5401 gggtagcccg ccgcgcagcc gatcccgcaa cggctcgaac agcccaaagg agcgcagcag
     5461 tttgtcgatg accgtggact ccccgcccgg aagcacgatg ccctgtatgc cgtgcagctc
     5521 gctctcgagc ttgaccggca cggcggtcgc gccaaccgcc tccagggcat ggacgtgctc
     5581 tcggacgtcg ccctgcaacg cgaggacgcc aatggtcggt gcggtagtca caaccagcca
     5641 ggctatctcg acgggcggtg cgcccagggc cacgaggggc cacggcgacg gagtgcggtg
     5701 aggttggctc gttccggctt gggtccttga gggagccgtg gccggctgcc caagggaacg
     5761 cggacaccgt tcgccgcgag ggtggccacc agcgggctgt cgtctatcgg tcgggactgg
     5821 agagcaccat cgacgtccgc cacccttgag atggtgatcc tggcgatcga gggtccgtag
     5881 accagcagtc gatccttctc gtcgatctgc atccataagt gttcggccgc gacctgcagg
     5941 gtgggccgcg agtggcctgg aacggtgaag gtgagccagc cgtcggccag ggcgaggtgc
     6001 cgaggccgcc gcactggcag gcgtgcgttg aaggcgagca cgtcgaaagt cgcgttcacc
     6061 gtggaacgct ctgccgacgg cgggatctgg gcggcccgtt cgcgctgtcg cagctccgcc
     6121 gcgaccggga gaaaacgcga ccaaccgaac cacgccaacc agacgctgaa cgccacggcg
     6181 gccaggatgc ccaccaccgt gagcgtgttg gagacccagt gatccgccat cggaacaatc
     6241 accgtgacca tcacatcagc gcccgcccag gttggggtcg gttcgcgagc cgcaccgagg
     6301 gtctaccagc cgcgctcggc gaggcggtgg gactgcggca gctcttcgac gttgatgccg
     6361 accatggcct cacccagacc gcgcgacacc ttggcgatga cgtcagcgtc gttgtagaac
     6421 gtcgtcgcct tgacgatggc ctcggcccgc tgcgcggggt tgcccgactt gaagatgccc
     6481 gagccgacga agacaccctc ggcacccagc tgcatcatca tcgccgcgtc agccggggtg
     6541 gcgatgccac cggcggtgaa cagcacgacg ggcagcttgc cggccagagc cacttccttg
     6601 accaagtcat acggcgcctg caactccttc gccgcgacat acaactcgtc gggggtcatc
     6661 gaccgcagcc ggttgatctc gccgcggatc tgacgcatgt gggtcgtcgc gttggacacg
     6721 tcacccgtgc ccgcctcacc cttcgagcgg atcatcgccg cgccctcggt gatccggcgc
     6781 agcgcctcgc cgaggttggt cgccccgcac acgaacggga cgttgaacgc ccacttgtcg
     6841 atgtggttgg tgtagtcggc cggggtcagc acctcggact cgtcgatgta gtcgacgccc
     6901 aggctctgca gcacctgcgc ctcgacgaag tgaccgatcc gggccttggc catcaccggg
     6961 atcgacaccg ccgcgatgat cccgtcgatc atgtcggggt cgctcatccg cgacacgccg
     7021 ccctgagccc ggatgtcggc cgggacccgc tccagcgcca tgacggcgac ggcgccggcg
     7081 tcctcggcga tcttggcctg atccggggt
//
LOCUS       DAORPW010000119         7002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2778_length_7002_cov_778.511876, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000119 DAORPW010000000
VERSION     DAORPW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2778_length_7002_cov_778.511876"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLB86_09465"
     CDS             <1..609
                     /locus_tag="PLB86_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRC65402.1"
                     /translation="GTESLQTFGGSGFLQEYPLEQYVRDAKIDTLYEGTTAIQGLDFF
                     FRKIVRDHGQSMTGLSMQIAQFAKDLGAQAGYFDSEREALGKALENVQGIVGYMAGAA
                     FQSDPRNGGDVKNLYKVGQNSTRLLLGAGDLIVGWLLMRQAEVAKAALDAGASPKDVD
                     FYTGKIAAAKFFVHQVLPRLAAERAMAESTDLSLMDVPEAAF"
     gene            809..1744
                     /locus_tag="PLB86_09470"
     CDS             809..1744
                     /locus_tag="PLB86_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65403.1"
                     /translation="MLDNPTTDVSQLAKTLKMKRGEVAAELDRLAALRLVTTDEGGVN
                     VVSPVAPDQAIESLLAQQERRLAQMQELVDTSRQNVAHYVEAFVDSRVRRDDHDLVEI
                     IDEAEVVRSRLYQLVREARVSAVTIHGGAPLPSDAIESSRRLDRELLERKVVLRAIVA
                     TPSLTSEAWRTHLVEASSIGMQVRVHPSPGPHTIVIDGSVALIPRTSRPGALILHGPD
                     LVAPVAQLFTQVWNAAQPWDDIVNLGAEGEFSEARLRQVVSLLAQGLKDDAIARKTAT
                     SVRTVRRLVAAATAALQAESRFQAGVEALRRGWVD"
     gene            complement(1892..3229)
                     /locus_tag="PLB86_09475"
     CDS             complement(1892..3229)
                     /locus_tag="PLB86_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRC65404.1"
                     /translation="MAAATPADRDRWADALRVGSLLVVIVGHWLMVAVTPTGEITNTL
                     NLIPWLQPVTWVLQVMPLFFLVGGVAHAHSLGSLARRGGTGPGGYAAFIRARAARLLR
                     PTLAFLAVWVLLGVIADRTGLTSGSSWQAALVVAALVMVPQLLWFVGIYLAVAAFAPA
                     LYRAHGRWGLWAVVALCSAALLVDLARFGAGLGILGNLNFALVWLALHQCGFAWRDGS
                     LTNGVAFVLVGVGVSGLALSIAVGPYPVSMVGLPGDEISNMSPPTVALLCQGLALIGI
                     AALARGPMARVLARPRVWRGVVTAGAFAMTAFLWHLTALLFTLLSAWALGILLPEVGS
                     AAWWWSRPVWFAVLAIPTAALVAIFIRFDRGPRSAAGVVTGQPRWVDWVAVAGVIVTV
                     FGILMVSIVGVDILGNRAQFFLFGMLTPGQAFIVLLVGLGLLRLAAPRAASRT"
     gene            complement(3501..4352)
                     /locus_tag="PLB86_09480"
     CDS             complement(3501..4352)
                     /locus_tag="PLB86_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HRC65405.1"
                     /translation="MRIAVVQVAHGDDERVAERVARVAATVRGLGGHDLVVLPELWPV
                     GGFDYRRWAEQAEAADGPIAATMAQAARDAGVVLHAGSIVERGPAPGPEGKWLWNTSL
                     VFGPDGSELARYRKIHRFGFATGEPELMEAGDEVVVFDLPVGVSAGGAGADGGPATRT
                     VRAGLSTCYDLRFPELYRRQLDLGAQVLLVPAAWPAARVGHWSLFARARAVEEQCLVI
                     ACNTAGTHCGVTMGGRSVVVLPTGDVVAEAGADDEEVLSVEVDLEVIERTRAVFPVLA
                     DRRLRMP"
     gene            complement(4358..6400)
                     /locus_tag="PLB86_09485"
     CDS             complement(4358..6400)
                     /locus_tag="PLB86_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRC65406.1"
                     /translation="MSIAASDKRPLLPLAPLLATGVVALAACLVVIGVGGAAAPAAPG
                     LQDPGAVVRWGLPIIRTVHDLSAALTVGLFFLAAVAIPDRASAALARAPRWGVAAGIT
                     WVVSGLIGVVLGFADIAGTPLGSSGFFAQFQRFVWSVETLREGLISAFLAAIAVTVAA
                     MWSSRRAVLWAGIVSVVAILPLALAGHGASTVEHETAVNGLAFHLVGTALWVGGLGAL
                     VLLRPVLGKWLPIVVERYSKVAAWSLLTVALSGVVSAAVRMSGLSDLATTYGAIILAK
                     VVAIVVLGQLGLAQRTKVIARLRETPSSAPLFARLIAVELVVMGAAIGIATALARTGN
                     PSLERPAPASLAEGLTNYPEPAAPDATSWLTMWRWDWLWGTVAVVAILLYVGGVVRLM
                     RRGDRWPVSRTIVWVLGWATFIWSVCGAPGVYGRVSFSWHMTSHMTIAMIVPILLVLA
                     APITLIARVESPRKDGTYGPREIVLGLVHSKYVAFWANPIVAGVNFVFGLIIFYYTPL
                     FELALTTHTGHVLMVIHFLLAGYLFAMVLIGVDPGPRKWAPALRLVVLFATMSFHAFF
                     GVAIQSSTSLLAPGFFSIIHIPWIPDPLLDQQQGGAIAWGIGDVPSLLLAMLLVLSWV
                     RSDDAEARRHDRQADRDHDADLTAYNARLASIAEHDAQEAARDAARLSGMPREGGS"
     gene            6495..6992
                     /locus_tag="PLB86_09490"
     CDS             6495..6992
                     /locus_tag="PLB86_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC65407.1"
                     /translation="MQATISPDAGPPAVTVGTGGALGAELVRLVKLLVAMRQHAPTTH
                     GAIDTTHYPVLFALANGPLRISDLAGCAHSDVSTVSRQVSFLVQHHLLAKVPDPADGR
                     AHRVSLTDEGRAAIDQALAARGQWLSAIMTDWSDEDARQFLHQVSRFTASLEAAHGRF
                     ERAAR"
BASE COUNT         1287 a         2489 c         2347 g          879 t
ORIGIN      
        1 ggcaccgagt cgttgcagac cttcggcggc tcgggcttcc tgcaggaata cccgcttgag
       61 caatacgtcc gcgacgccaa gatcgacacc ctctacgaag gcacgaccgc gatccagggc
      121 ctggacttct tcttccgcaa gatcgtccgc gaccacgggc agtcgatgac cggcctgtcg
      181 atgcagatcg cccagttcgc caaggacctc ggcgcgcagg cgggctactt cgactccgag
      241 cgcgaggccc tcggcaaggc gctggagaac gtccagggca tcgtcggtta catggccggc
      301 gccgccttcc agtccgaccc ccgcaacggc ggcgacgtca agaacctcta caaggtcggc
      361 cagaactcca cccgtctgct gctcggtgcc ggtgacctca tcgtcggctg gctgctcatg
      421 cgccaggccg aggtcgccaa ggccgcgctc gacgcgggcg cctcccccaa ggacgtcgac
      481 ttctacaccg gcaagatcgc cgccgcgaag ttcttcgtcc accaggtcct gccgcgcctg
      541 gccgccgagc gcgccatggc cgagtcgacc gacctgtcgc tcatggacgt cccggaggcc
      601 gccttctagc ctcaccccac gaagcgcgcg acgcggcata cctgatccct ggtatgccgc
      661 gtcgccgctg tgtggtgacc tcgcacccgg caccggtcac tgagcgccat cgaacggttc
      721 caactacggt gggcacaaca gtcgatcagg ggccgacatg ctagagatct ttggcgttca
      781 cggaacgacg gaacgggttt atcgagccat gcttgacaac ccgaccacag atgtctcgca
      841 actcgccaag acgctgaaga tgaagcgcgg cgaggtcgct gccgagctcg atcggctagc
      901 cgcgctcagg ctcgtcacca ccgacgaggg cggcgtcaac gtcgtcagtc ccgtcgctcc
      961 cgaccaagcc atcgaatcgc tgctcgcgca acaggaacgc cgcctcgcgc agatgcagga
     1021 gttagtcgac acttctcgtc aaaacgtggc gcattacgtc gaagctttcg tcgacagtcg
     1081 cgttcgacgg gacgaccacg acttagttga aatcatcgac gaagcggagg tcgtgcgctc
     1141 acgcctctac caactcgtcc gcgaagcgcg ggtgagtgcc gtcaccattc acggtggggc
     1201 accgcttccg agcgatgcga tcgaatcgtc gcgtcggctg gatcgcgagt tgctcgagcg
     1261 gaaggttgtc cttcgggcca ttgtcgcgac cccatcgctg acctcggaag cctggcgcac
     1321 ccacctggtc gaggcgtcct cgatcgggat gcaggtgcgg gtccacccca gcccagggcc
     1381 gcacaccatc gtcatcgacg gctccgttgc gctcatccca cggaccagcc ggccgggtgc
     1441 gttgatcctg cacggaccgg acctcgtcgc gccggtcgcg caactgttca cccaggtgtg
     1501 gaacgccgcg cagccctggg acgacatcgt caacctgggt gccgagggcg agttctccga
     1561 ggcccgcctt cgtcaggtcg tcagcctgtt ggcccagggg ctcaaggacg atgccatcgc
     1621 ccgcaagaca gccacctcgg tccggacagt gcgccgactg gtggcagcgg ccaccgcggc
     1681 gttgcaggcc gagagccgct tccaggccgg agtcgaagcg ctccgccgcg gctgggtcga
     1741 ctgacccgcg ccgccgagac tgacccgcgc tgccgaggcg gcaggggtat gccgcgacca
     1801 tggcgggtct ggcgggaagc acgagcgagc gcagcgagag agcgcgcatg gtctagccga
     1861 ggcatacccc tgccgtcgac cacgaggaga ctcaggtgcg agaggccgcg cgaggggcgg
     1921 ccaggcgcag caagcccaac ccgaccagca ggacgatgaa cgcctggccg ggggtcagca
     1981 tcccgaagag gaagaactgc gcccggttgc cgaggatgtc gacgccgacg atcgacacca
     2041 tgaggatgcc gaagaccgtc acgatgaccc cagcgaccgc gacccagtcg acccaacgcg
     2101 gctgccccgt gaccaccccg gccgccgagc gaggaccgcg gtcgaaccgg atgaagatcg
     2161 cgaccagggc agcggtcggg atcgcgagca cggcgaacca caccggccga ctccaccacc
     2221 aggctgcgct gcccacctca ggcaacaaga tgccgagcgc ccaggcgctc aacagggtga
     2281 agagcagcgc cgtcagatgc cagaggaagg cggtcatcgc gaacgcaccc gcggtgacca
     2341 caccgcgcca cacccgtggg cgcgccagca ctcgggccat cgggccacgc gccaacgccg
     2401 cgatcccgat gagcgccagg ccctggcaca gcagggcaac cgtcggcggg gacatgttcg
     2461 agatctcgtc gccgggcagc ccgaccatcg acaccggata gggccccacg gcgatcgaga
     2521 gcgcgagccc cgagactccg acgccgacga gcacgaaggc caccccattg gtcaacgagc
     2581 cgtcgcgcca ggcgaacccg cactggtgca gcgccaacca gacgagcgcg aagttgaggt
     2641 ttccgaggat gcccaggcct gcgccgaacc tcgcgagatc gacgagcagc gctgctgagc
     2701 acagggcaac gaccgcccag agcccccatc gaccgtgggc gcgatagagc gcgggggcga
     2761 aggccgccac ggccaggtag atcccgacga accacagcaa ctgcggaacc atgacgagcg
     2821 ctgccacgac gagcgccgcc tgccacgagg accccgacgt cagcccggtg cggtcggcga
     2881 tgacaccgag caggacccac acggcaagga acgcgagcgt tgggcgcaac aggcgcgcgg
     2941 cccgggcccg aatgaacgcg gcataccctc cgggaccggt gccgccgcgg cgggcgaggg
     3001 agccaagcga gtgggcgtgc gcgacgccgc ccacgaggaa gaacagtggc atgacctgca
     3061 ggacccaggt gaccggctgc agccagggga tgaggttgag ggtgttggtg atctcgccgg
     3121 ttggtgtcac ggccaccatg agccagtgcc cgacgatgac gacgagcagg gagccgacgc
     3181 gcaaggcatc ggcccaccgg tcgcgatcag cgggcgtggc cgcggccacc gacgcaacgg
     3241 tcgacggagc gtctgatgga gcggccgacg caacggtcga cccaagattc ggggccggag
     3301 actcgggcgc gggaggcgta gctggcgaac tcacgcggcc cactgtccca catggcaccc
     3361 tccgaggcct caagctggcg ggcttcccgg ctgggtcgag gtattccgcc atcgtcaggt
     3421 gatggcggaa tacctcgacc cggcgagagg tgtgacgctt gtatgaggga ctatggcgcg
     3481 actctgcgga ggatccgcgg tcagggcatc cggaggcgac ggtcggcgag caccgggaag
     3541 acggcccggg tgcgctcgat gacctcgagg tcgacctcga cggacagcac ctcttcgtcg
     3601 tcagcaccgg cctcggcaac cacgtcgccg gtgggaagca cgaccaccga ccgaccgccc
     3661 atcgtcaccc cgcagtgggt gccagccgtg ttgcacgcga tgacgaggca ttgttcctcg
     3721 acggcacgcg ctcgggcgaa gaggctccag tgcccgaccc gagcagccgg ccaggccgcg
     3781 gggacgagca acacctgtgc accgaggtcg agttggcggc gatagagctc ggggaagcgc
     3841 aggtcgtaac aggtggacaa cccggcccgg accgtccggg tcgcaggtcc accatcagca
     3901 ccagcaccgc cagcgctcac acccaccggc aggtcgaaca cgaccacctc atcgccggcc
     3961 tccatgagct ccggctcccc cgtcgcgaac ccaaaccggt ggatcttgcg atagcgcgcc
     4021 aactccgacc cgtctgggcc gaacaccagc gaggtgttcc acagccactt gccctccggc
     4081 ccgggcgccg gaccgcgctc gacgatcgac ccggcgtgca acaccacgcc cgcatcgcgc
     4141 gcggcctgcg ccatcgtcgc cgcgatcggc ccgtcagcgg cctcggcctg ctccgcccac
     4201 cgccggtagt cgaacccgcc caccggccac agctcaggca acaccaccag gtcgtgcccg
     4261 cccagtcctc gcaccgtcgc cgcgacccgg gcgacccgct cggcgaccct ctcgtcgtcg
     4321 ccgtgggcga cctggacgac cgcgatccgc agcggggtca tgagccgccc tcgcgcggca
     4381 taccggagag gcgggccgcg tcacgtgcgg cctcctgcgc gtcatgttca gcgatgctcg
     4441 ccaggcgcgc gttgtatgcc gtgaggtcgg catcgtggtc acggtcggct tggcggtcgt
     4501 gccggcgtgc ctcggcgtcg tccgagcgaa cccaggacag caccaggagc atggcgagca
     4561 gcagggaggg gacatcgccg atgccccacg cgatggcgcc gccctgttgc tgatccagca
     4621 gcgggtcggg gatccacggg atgtggatga tcgagaagaa ccccggcgcg agcagcgacg
     4681 tgctcgactg gatggcgacg ccgaagaacg cgtggaagga catcgtcgcg aacaacacga
     4741 ccaggcgcag ggccggcgcc cacttgcggg ggcctgggtc gacgccgatg agcaccatgg
     4801 cgaagagata cccggccagg aggaagtgga tgaccatgag gacgtggccg gtgtgcgttg
     4861 tgagggccaa ctcgaacagc ggcgtgtagt agaagatgat cagcccgaag acgaagttga
     4921 ccccggccac gatcggattg gcccagaacg caacgtattt cgagtggact aggccgagga
     4981 cgatctctcg tgggccatag gtgccgtcct tgcgaggcga ctccacgcgg gcgatgaggg
     5041 tgatcggcgc cgcgaggaca agcaggatcg ggacgatcat ggcgatcgtc atgtggctgg
     5101 tcatgtgcca ggagaaggac acccgcccat agacgcccgg cgcgccacac accgaccaga
     5161 tgaacgtggc ccagcccaac acccagacga tcgtgcgact cacaggccac cggtcgccgc
     5221 ggcgcatcag acgcacgaca ccaccgacgt agagcaggat cgccacgacg gcgaccgtcc
     5281 cccagagcca gtcccagcgc cacatcgtca gccacgacgt ggcatcagga gcggccggct
     5341 cggggtagtt ggtcagtccc tcggcgaggc ttgccggggc cggacgctcc aggctggggt
     5401 tgcccgtgcg ggccagggcc gtggcgatgc cgatggccgc gcccatgacg accaactcga
     5461 ccgcgatgag acgggcgaaa aggggcgcgg acgacggcgt ctcgcgcagc cgcgcgatca
     5521 ccttggtccg ctgagccagg cccagttggc ccaggacgac gatcgccacg accttggcca
     5581 ggatgatcgc gccgtaggtc gtggccaggt cactgagccc tgacatccgg acggccgcgc
     5641 tcaccacgcc ggagagagca acggtgagca gcgaccaggc ggcgaccttg gagtagcgct
     5701 cgacgacgat cggcagccac ttgcccaaca ccggccgaag caggaccagc gcgcccaggc
     5761 caccgaccca gagcgcggtg ccgacgaggt ggaaggcgag cccgttgacg gcagtctcgt
     5821 gctcgaccgt gctcgcgccg tggccggcca gggccaacgg gaggatggcg acgaccgaga
     5881 cgatccctgc ccagagcacg gcccgtcggc tgctccacat cgctgcgacg gtgaccgcga
     5941 tcgcggcgag gaacgcgctg atgaggccct cgcgcaacgt ctcgaccgac cagacgaagc
     6001 gctggaactg ggcgaagaat cccgaggacc ccagcggcgt gccggcgatg tcggcgaagc
     6061 ccaacaccac accgatcagc ccggagacca cccacgtgat gccggccgcg acgccccacc
     6121 gaggggcccg ggccaacgct gcggaggcgc ggtcggggat cgcgaccgcg gccaggaaga
     6181 acagcccgac ggtcagagcg gcagacaggt catggaccgt ccggatgatc ggcaatcccc
     6241 accggacgac ggctcccggg tcctgcaatc cgggagcagc gggagcggcc gcccctccaa
     6301 ccccgatgac gacaaggcac gccgcgagcg cgaccactcc ggtcgcgagc agcggggcca
     6361 acggcaggag agggcgcttg tccgaggcag caatggacat gactccaccc taggtctgtt
     6421 ccctcacgct gcagcacccg gtgcccctcc ccgaaccatt tggttgcggc gtgcaaacag
     6481 tttatagttg cgtggtgcaa gcaactatct ccccggacgc cgggccgccg gccgtgacag
     6541 tcggcaccgg aggggccctc ggtgccgagc tcgtccgact ggtcaagctc ctcgtcgcga
     6601 tgcgccagca cgcacccacc acccacggag cgatcgacac gacgcactac ccggtcctgt
     6661 tcgccctcgc gaacggaccg ctgcggatct ccgatctcgc cgggtgcgcc cattccgatg
     6721 tctcaacggt gagccgtcag gtgtccttcc tcgtccagca ccacctgctc gccaaggtgc
     6781 ccgaccccgc cgacggacgg gcccaccggg tctccctcac cgatgagggc cgagccgcca
     6841 tcgaccaggc cctcgccgcc cgcggccagt ggctctccgc catcatgacc gactggtccg
     6901 acgaggacgc tcgccaattc cttcaccagg tcagcaggtt caccgcgtcg ctggaagccg
     6961 cccacgggcg cttcgagcgc gcggcgcgct gaccggcata cc
//
LOCUS       DAORPW010000120         6908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2848_length_6908_cov_769.778783, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000120 DAORPW010000000
VERSION     DAORPW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2848_length_6908_cov_769.778783"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..682)
                     /locus_tag="PLB86_09495"
     CDS             complement(44..682)
                     /locus_tag="PLB86_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRC65408.1"
                     /translation="MNINRLAERARTARDELDEILDAGWVGTLSVSVDGSPQVVPILY
                     ARVGDAVILHGSTGAGSLRAAAAGSPVAFCVAHLDGFVYAASLFESSANYRSAVVYGS
                     CERLTGDAAEAALVGLSEHVMPGRAGEVRANTAKERAATIVLRLPIVDGQWLAKARTG
                     SASVEEGDDPSVWTGVLPVRTVLGLPQPSPETAERGIPVAGSVARRVADQSG"
     gene            766..1944
                     /locus_tag="PLB86_09500"
     CDS             766..1944
                     /locus_tag="PLB86_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HRC65409.1"
                     /translation="MPRRSRDAGREVAPIKELDRREVAGAVADLGVLVPIVVALILVN
                     GLSASAVLLPAGLLYVAAGWFYRLPIPVQPLKAFGAIAIAQGLGADVIAAGSLLMALV
                     FIPLGLSGLLDRAAAIFPKPLVRGVQLSVGILLIRLAWGLVVEPPRQFADAGAPTALL
                     VAGALVAGAAAYVWRARGITVVLVGIALVWVVARAHLPMTLGPSELRLHTPSASDFAT
                     AAVVLVVPQLPLTFANSCIATADAARGYFGAAASRVTAGRLAVSLGLGNVLAGLLGGM
                     PVCHGAGGLTAHRSFGARTGRAPIFLGTILVVLALVAGQVTGGALAGFPVWILAALLG
                     VAGVLHIQLLRDLRGRTEWVFAIAVGVLGALVNLAVALAVGLSAWWVAAALRRSRASG
                     "
     gene            complement(2027..2923)
                     /locus_tag="PLB86_09505"
     CDS             complement(2027..2923)
                     /locus_tag="PLB86_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65410.1"
                     /translation="MDADPSVDARLDEAVDELYGLTGDAFLPRRGQLAAAARAGGDTE
                     LAKAITALRKPTQSAWAINLLSRECADDLDRLADLAARLRTAQERLDGAALLTLGRER
                     TALVDELVAQAHVVVTQAGAKFSPAMARDIGTTFVAALASTEATAAVTSGRLTRALEY
                     AGFGEIDLTEATARPLRLVRSDERASLMSSGVRADAHRGASSPDGALADDADPEVSAA
                     EADLHDAMATATAAASRHGVLSAELELAENRVAQLQAELARARAHRDKTADALGEADS
                     AQAAAQRELRRARTALEDLRNG"
     gene            2957..3721
                     /gene="dapB"
                     /locus_tag="PLB86_09510"
     CDS             2957..3721
                     /gene="dapB"
                     /locus_tag="PLB86_09510"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRC65411.1"
                     /translation="MSQPKVAVIGSRGRMGATVCRAVEAASDLEFVGGFDMGDELGDL
                     GGADVVVEFSVPSASLANVVACVSRGVHVVVGTTGWDDAKLDELRQQLLAAPEARAGG
                     PVGVLIAPNFALGAILMMSFAAQAARFFESVEVVELHHPNKVDAPSGTAARTAELIGA
                     ARDAAGVGPVPDATTTDPDGARGARVHGIPVHSLRVRGLVAHQEVILGNVGETLTIRH
                     DSLDRESFMAGVLAGVRGVAGHPGVTVGLEHYLGLG"
     gene            3859..4569
                     /locus_tag="PLB86_09515"
     CDS             3859..4569
                     /locus_tag="PLB86_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65412.1"
                     /translation="MKRAAIVLASAIAVAAIGGCSSTATTTSTTPAATPATASAAPTA
                     GRMGSGGPPSDRGPGNGVGGGMGNGNGPARATNGAVTAAPPAGTVAAAAWEALMSPEG
                     EYAAAASYQAVIAKFGDVEPYVSIEAMEQNHIQALTRQLSRFGFVPPANPYAGTIAAP
                     ADLVTAAQAWVDGERKNVALYDRLAAAATGDAGVTRVFTNLRRSSLDAHLPLFEAAVK
                     AGGSLTEPQMAELGFVRH"
     gene            complement(4645..4959)
                     /locus_tag="PLB86_09520"
     CDS             complement(4645..4959)
                     /locus_tag="PLB86_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRC65413.1"
                     /translation="MSLWFTVLAACALAFLTKLAGHYVPSAKLEGRRMRRVTALLPVA
                     LLTSLVVIQTFAASGGGFTVDARIVGLAAAVIALLLRAPFIVVVIIGAAVAAGLRALG
                     WN"
     gene            complement(4956..5720)
                     /locus_tag="PLB86_09525"
     CDS             complement(4956..5720)
                     /locus_tag="PLB86_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRC65414.1"
                     /translation="MREEAGKEGAEASGQRPGDLDPATRTSVLRQAWSVGIATGAYGI
                     SFGALGVASGLSVAQTCALSLLMFTGGSQFAFVGVIGAGGTGVAAIATATLLGIRNGL
                     YGLQVSEFLHDRGWRRGVAAHLTIDESTAVGIAQPTVRASQLGFWQTGIAVFVFWNLF
                     TLLGAWLGNALGDPRRWGLDAAAAAAFVALLWPRLHSRDAAATAVVAVVLTVAVAPWA
                     PAGIPVLIAAFAAVAVGLWPRAAARDDDSTGGEVAP"
     gene            5845..6243
                     /locus_tag="PLB86_09530"
     CDS             5845..6243
                     /locus_tag="PLB86_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65415.1"
                     /translation="MAVVVAVVVALAGLTACSSAPVGETPESTVAGFYRALGAKDSAA
                     ACALVAYDGKPLAGDDVLLCRSGFDTIITDVATPEELAALTSASVTGAAVDGDQATVT
                     ADQMTGVPAAYREDVNLVRVGGRWYIESPL"
     gene            6320..>6908
                     /gene="paaA"
                     /locus_tag="PLB86_09535"
     CDS             6320..>6908
                     /gene="paaA"
                     /locus_tag="PLB86_09535"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRC65416.1"
                     /translation="MYGNDFAEPDDPGAGPLNAADGGAVEAAAAHVDTKAREGLASFD
                     AIIAADQRIEPRDAMPEEYRQTLIRQIAQHAHSEIIGMQPEGNWITRAPSLRRKAILI
                     AKVQDEAGHGLYLYSAAETLGITREELLDRLHTGKQKYSSIFNYPTLTWADCGAIGWL
                     VDGAAIMNQVPLCRCSYGPYARAMIRTCKEESFHQR"
BASE COUNT          978 a         2453 c         2469 g         1008 t
ORIGIN      
        1 ccaaccccga ggcgaacccc tggttcgacg cgggccctgc cgcctaaccc gactggtccg
       61 cgacccgacg ggccaccgac cccgccaccg gtatgccgcg ctcggccgtc tcgggggagg
      121 gctgcggaag gccgaggacc gtgcgcaccg ggaggacccc ggtccatacc gacgggtcgt
      181 ccccctcctc cacgcttgcc gacccggtgc gtgccttggc cagccattgg ccgtcgacga
      241 tgggcaggcg caggacgatc gtcgccgcgc gttccttggc ggtgttggcc cggacttcac
      301 cagcgcgccc aggcatgacg tgctccgaga gccccaccaa ggcagcctcg gcggcgtcgc
      361 cggtcaggcg ctcacagctg ccatagacga ccgccgagcg gtagttggcg ctggactcga
      421 acagcgacgc ggcatacacg aagccgtcga gatgagccac gcagaaggcg accggcgacc
      481 cggccgcggc ggcgcgcagg ctgccggctc cggtggagcc gtggaggatg accgcgtcgc
      541 cgacgcgcgc atagaggatc gggacgacct gcgggctccc gtcgacgctc accgacaggg
      601 tgcccaccca accggcgtca aggatctcgt cgagctcgtc gcgagcggtg cgggcgcgtt
      661 cggcgaggcg gttgatgttc atgggtcgat cctctcggtt tgcgccaccc ggtcgcacag
      721 acatttcacg tcggggaggc tgcgtcggca tgggaccatc ggcgggtgcc ccgccgatcc
      781 cgagacgccg gccgcgaggt ggcgcccatc aaggagctgg accgccgcga ggtcgccggt
      841 gccgtcgctg acctcggcgt gctcgtcccg atcgtcgtgg cgctcattct tgttaacggg
      901 ctctcggcca gcgcggtgct gttgccggcc ggcctgctct atgtggccgc gggctggttc
      961 taccgtctgc cgattcctgt gcagccgctc aaggccttcg gagcgatcgc catcgcccag
     1021 gggctcggcg cggacgtcat cgcggccggg tcgctgctca tggcgctggt attcatcccg
     1081 ctcgggctta gcggtctgct cgatcgtgcc gcggcaatct ttcccaagcc gctcgtccgt
     1141 ggagtgcagc tgtcggtcgg catcctgctc atccgccttg cctggggtct ggtggtcgag
     1201 ccgccccgcc agttcgccga cgcaggagca ccgaccgcgc tcctcgtggc cggtgctctc
     1261 gtcgccgggg ccgcggcata cgtctggcgg gcccggggga tcaccgtcgt cctcgtcgga
     1321 atcgcgctgg tgtgggtggt tgcccgagcc cacctcccga tgaccctcgg cccctcggaa
     1381 ctgcggctgc acacgccgtc tgcaagcgac tttgctacgg ccgcagtggt gttggtcgtg
     1441 ccgcagttgc cgctcacctt cgccaactcc tgcatcgcca ccgccgacgc agctcgcggc
     1501 tacttcggtg cagccgcatc gcgggtcacc gccggacggc tggccgtgtc gctcgggctg
     1561 ggcaacgtcc tcgccggact cctcggaggt atgccggtct gtcacggcgc ggggggcctc
     1621 accgctcacc ggtccttcgg cgctcggacc ggccgggcac cgatcttcct cggcaccatc
     1681 ctcgtcgtgc tggccttggt ggccggtcaa gtcaccgggg gtgcgctcgc gggtttcccc
     1741 gtgtggatcc tcgccgcgct gctcggggtt gccggagtgc tgcacatcca actcctgcgt
     1801 gatctgcggg gtcgcaccga gtgggtcttc gccatcgcgg tcggcgtgct gggagcgttg
     1861 gtgaacctcg cggtggcgct ggcagtgggg ctgagcgcct ggtgggtggc tgccgccttg
     1921 cgccggagcc gggccagcgg ctgaccactg caggatgacg ggggccgtga gcaggcggtc
     1981 ggccggggct tggccagtgc ccggggagtg cacagccggt tgacactcag ccgttgcgca
     2041 ggtcctcaag tgccgtgcgg gcccggcgca actcgcgttg ggctgcagcc tgcgcgctgt
     2101 cagcctcgcc gagcgcgtcc gcggtcttgt cccggtgcgc tcgggcccgg gcgagctcgg
     2161 cctggagttg agcaacgcgg ttctcggcga gctcgagctc ggcgctgagg actccgtgac
     2221 gggatgccgc cgctgtggcg gtggccatgg cgtcgtgcag gtcggcctcg gccgccgaca
     2281 cctcgggatc ggcgtcgtcc gcgagtgccc catccggtga cgaggcgccc cggtgggcgt
     2341 cggcacggac gcccgacgac atcagcgagg cgcgctcgtc ggatcgcacg agacgaagcg
     2401 ggcgagcggt ggcttcggtg aggtcgatct cgccgaaacc ggcatactcc agggctcggg
     2461 tgagccgacc ggaggtgacg gccgccgtgg cctcggtgga ggcaagggcc gcgacaaagg
     2521 tggtgccgat atcgcgcgcc atcgccgggc tgaacttggc ccccgcctgg gtgacgacca
     2581 cgtgggcctg cgccaccaac tcgtcgacga gcgcggtgcg ttcgcggccg agggtgagca
     2641 gcgcggcgcc gtcgaggcgc tcctgagccg tgcgcagccg cgctgcgaga tccgccagcc
     2701 gatccagatc gtcggcacac tcgcgggaga gcaggttgat cgcccaggcc gactgggtgg
     2761 gcttgcgcag cgccgtgatc gccttggcga gctccgtgtc accaccagcc cgggcggccg
     2821 ccgccaactg acctcggcgc ggcaggaacg cgtcgccggt caacccgtag agctcgtcga
     2881 ccgcctcgtc gaggcgggcg tccacgctcg ggtcggcgtc catgggcgga atcgtcccac
     2941 ggctaccctt gcgctcatga gccagccgaa ggtcgccgtc atcggatccc gcggtcggat
     3001 gggagcgacg gtatgccgcg ccgtcgaggc cgcgtccgat ctggagttcg tcggtggttt
     3061 cgacatgggc gacgagctgg gcgatctcgg cggagctgac gtcgtcgtcg agttctcggt
     3121 gccctcagcg tcgctggcca atgtcgtcgc ctgcgtcagc cggggtgtgc acgtcgttgt
     3181 ggggacgacc gggtgggacg acgccaaact cgacgagttg cgccaacaat tgctcgcggc
     3241 gcccgaggcg cgcgccggcg gaccggtcgg ggtgctcatc gcccccaact tcgccctcgg
     3301 cgccatcctc atgatgtcgt tcgccgctca agcggcgagg ttcttcgagt cggtcgaggt
     3361 cgtcgaactg caccacccca acaaggtcga tgcaccctcg ggcaccgccg cgcgcaccgc
     3421 cgagctcatc ggggccgcgc gggacgcagc cggagtcggg ccggttcccg acgccaccac
     3481 gaccgatccg gacggtgccc gcggcgcgcg cgtgcacggc ataccggtgc actcgctgcg
     3541 agtgcgcggg ctggtggcgc accaggaggt catcctcggc aacgtggggg agacgctgac
     3601 gatccggcac gactcgctgg accgcgagtc gttcatggcg ggcgtgctcg ccggcgtgcg
     3661 aggcgtcgcc gggcatccgg gcgtcacggt cggcctggag cactacctcg gcctcggctg
     3721 acgggggcgg cccgcggcgc cgctgcggtc gctcgcccct cggcgagatc tctcgcaacg
     3781 cccacgcgcg cttcacagtg gcttcatgaa tgcctcggag actgaggtcg atcccagctc
     3841 cgagcaaagg aacccgtcat gaagcgcgca gccatcgtcc tcgcctcagc catcgccgtg
     3901 gccgcgatcg gtgggtgttc cagcaccgcg acgaccacca gcaccactcc cgccgcaacg
     3961 ccggcgactg cctcggcggc cccgaccgct gggcggatgg gctccggcgg cccgccgtcc
     4021 gaccgtggcc ccggaaacgg tgtcggaggc gggatgggca acggaaacgg ccctgcccgc
     4081 gccacgaacg gcgcggtcac cgccgcccca cccgccggca ccgtcgccgc ggcagcctgg
     4141 gaggcgctca tgagcccgga aggggagtat gccgccgcag cctcctatca ggcggtcatc
     4201 gcgaagttcg gagacgtcga gccctacgtc agcatcgaag cgatggagca gaaccacatc
     4261 caggcgctga cccggcaact gagtcggttc ggcttcgtcc cgccggccaa cccctatgcc
     4321 ggcacgatcg cggccccagc cgacctggtc accgcggccc aggcgtgggt cgacggggag
     4381 cgcaagaacg tcgcgctcta tgaccggctc gcggcggccg cgaccggcga tgccggggtg
     4441 accagggtct tcaccaacct gcgccgttcc tcgctcgacg cccacttgcc cctgttcgag
     4501 gcggcagtga aggccggcgg ctccctcacc gagccgcaga tggccgagtt ggggttcgtg
     4561 cggcactgac gtcgagtggt gtgatgagca ggacgttggg ctggaggtgg ccggttggac
     4621 cggtcagttc cagcccaacg cgcgttagtt ccagcccaac gcgcgaagtc cggcggcgac
     4681 ggcagcgccg atgatgacga cgacgatgaa cggcgcgcgc agcaacagcg cgatcacggc
     4741 ggcggccagg ccgacgatcc gggcatcgac ggtgaagccg ccaccggagg cggcgaaggt
     4801 ctggatgacg accaacgacg tgagcagcgc cacgggcagc agggccgtga cccggcgcat
     4861 ccgccggccc tcgagcttgg ccgacggcac gtagtgcccg gcgagtttgg tgaggaacgc
     4921 gagcgcgcag gccgcgagga cggtgaacca gaggctcacg gcgcgacctc cccaccggtg
     4981 ctgtcgtcgt cgcgggcagc agcgcgcggc cacagcccga cggccacggc cgcgaacgcg
     5041 gcgatgagca cgggtatgcc ggccggcgcc cacggcgcga cggccacagt gagcacgacc
     5101 gccaccacgg cggtggcggc cgcatcccga gagtgcagcc tcggccagag cagggcgacg
     5161 aaggcagcgg cggcggctgc gtccaggccc catcggcgtg ggtcgcccag ggcattgccg
     5221 agccacgccc cgaggagggt gaacaggttc cagaagacga agacggcgat ccccgtctgc
     5281 cagaagccca gctgggacgc ccgaaccgtg ggctgagcga tcccgacggc cgtcgactcg
     5341 tcgatcgtca ggtgggcggc gaccccacgt cgccagccgc ggtcgtggag gaactccgac
     5401 acctgcaggc cgtagagccc gttacggatc ccgagcaggg tcgccgtggc gatggccgcg
     5461 acccccgtgc caccggcgcc gatgacgccg acgaaggcga actgcgaccc tcccgtgaac
     5521 atcagcaggg acagggcgca ggtctgcgcc acgctcagcc ccgacgccac gcccaaggct
     5581 ccgaacgaga tcccgtaggc ccccgtcgcg atccccaccg accaggcctg gcgcagcacc
     5641 gacgtgcgcg tcgcgggatc gaggtcgcct ggccgttgcc cgctcgcctc cgcgccctcc
     5701 ttgcccgcct cctcgcgcat ccgccgatgg tgacagaggg ccgtgtgcgc atgtttggat
     5761 ggtccggtgg gtgatcgtct accgcgtgtt cgggcccttg tgggcgccaa agccgacgcc
     5821 gctgccaccg ccagagccgt cgccttggcg gtcgtcgtgg cggtcgtcgt ggcgctggcc
     5881 gggttgacgg cgtgctcgtc agcacccgtc ggcgagacgc cggaatcgac ggtcgcgggg
     5941 ttctatcgcg cgctgggcgc caaggactcg gccgccgcgt gcgcgttggt cgcctacgac
     6001 ggcaagcccc tggcaggcga tgacgtcctg ctgtgccgat cgggcttcga cacgatcatc
     6061 acggacgtgg caacacccga ggagttggct gctctcacct cggcgtccgt cacgggtgca
     6121 gcggtcgacg gcgaccaggc aacggtcact gccgaccaga tgaccggcgt gcccgcggca
     6181 taccgggagg acgtgaacct cgtgcgcgtg ggcggacgct ggtacatcga aagcccgctc
     6241 tgaccttggt ctgacggctg ttgtgatgtt cacccctcca gagggggcga ggcgatcccg
     6301 gcccggccat actgtgctca tgtacggcaa cgacttcgcc gagcccgacg acccgggggc
     6361 aggccccctc aacgcggcgg acggaggagc ggtcgaagcc gcggcggccc acgttgacac
     6421 caaggcccgt gaaggcctgg cgagcttcga tgcgatcatc gccgccgatc aacggatcga
     6481 gccgcgcgac gcgatgcccg aggaatatcg ccagaccctc atccgccaga tcgcccagca
     6541 cgcgcactcc gagatcatcg ggatgcagcc ggagggcaac tggatcaccc gggcaccctc
     6601 gctgcggcgt aaggcgatcc tcatcgccaa ggtccaggac gaggccggcc acgggctcta
     6661 cctctactcc gcggcggaga ccctcggcat cactcgggag gaactgctcg accggctgca
     6721 caccggcaag cagaagtact cctcgatctt caactatccg acgctcacct gggccgactg
     6781 tggcgccatc ggctggctcg tcgacggcgc ggcgatcatg aatcaggtgc cgctgtgtcg
     6841 gtgctcctat ggtccctacg cccgggcgat gatccgcacc tgcaaggagg agtccttcca
     6901 ccagcggc
//
LOCUS       DAORPW010000121         6844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2897_length_6844_cov_718.238032, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000121 DAORPW010000000
VERSION     DAORPW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2897_length_6844_cov_718.238032"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLB86_09540"
     CDS             <1..188
                     /locus_tag="PLB86_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRC65417.1"
                     /translation="EQVRLSWHASEDGPRSLVDLHLAPQGEQTYVSIRHEHVEGDLDS
                     LKARWAAALSRLEAAAG"
     gene            304..>2057
                     /gene="sulP"
                     /locus_tag="PLB86_09545"
     CDS             304..>2057
                     /gene="sulP"
                     /locus_tag="PLB86_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HRC65418.1"
                     /translation="MTAAPASPEPSLQEPPPHAGLQRWLPILTWLPTYERRWIKDDLL
                     AGLGLTALLVPQGMAYAQLAGLPPITGLYTSIMCLVAYAVFGPSRVLVLGPDSSLGPM
                     IAASILPLVAANGDPMRAVVLASAQSVLVGIFMIAAGAAKLGFVADLLSKPTMVGYMN
                     GLALTILVGQVRKLCGFSISADGLIGEIVATVEKILAGATNLWALGIGVVALVIMRVL
                     QKTLPQVPAVLVAVIVGIGASTYLDLPGKGVKVVGDLPQGFPPFTLPLVSVGDIGLLV
                     VGALGITLVALADTISTASAYARRTKQPVDGNQEMVGIGAANVLAGLFQGFAVSTSGS
                     RTAVAFKAGARSQVTGVVGALAILLLLVVAPNLLSNLPQPVLGAIVILAAFSLAEVPA
                     ARKLWSQRRTEFWLSIAATLAVAVMGVLPGIGVAVGLSILNVFRRAWWPYQAVLGREK
                     GHPGYHDMTHRPRLTSMPGLIIFRFDAPLFFANARTFGEQITRLAATEPAPKWILVAA
                     EPISDIDTTAADMLASLVADLDERGTKLAFAELKSVVRGKLEAYALDGVIDNVLYFPT
                     LNAAGKAYRAQFGTDWGDT"
     assembly_gap    2058..2157
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2158..2655
                     /locus_tag="PLB86_09550"
     CDS             <2158..2655
                     /locus_tag="PLB86_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRC65419.1"
                     /translation="GGTDQAVYAVARDELAHWAAEVGRDLPPGAFGENLTITGLDVDG
                     ALVGEIWAIGDVVRLQVTGPRIPCATFARAMGEERWVKRFTVRGRTGAYLRVLTPGPV
                     QAGDAVVVESRPDHEITVPLMFRALTTERHLLPRLSEVGPDLSEEGRRELAAYREGTT
                     DGRMP"
     gene            2758..2955
                     /locus_tag="PLB86_09555"
     CDS             2758..2955
                     /locus_tag="PLB86_09555"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRC65420.1"
                     /translation="MKLNVGTTDRKIRIAIAIVAAILAVVVGGTFGVVLWVVAAIMLA
                     TGIVRVCPLYLPFKISTNKGA"
     gene            3025..3834
                     /locus_tag="PLB86_09560"
     CDS             3025..3834
                     /locus_tag="PLB86_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC65421.1"
                     /translation="MGITHEEITAAVPGLLRYARVLTRDAATAEDLVQDTVVRALERA
                     ADFRGDSSAATWLHRILHHRFVDVVRASRSDPVDAEELAERVEADWRDDSYTVDAETV
                     LLRAETAEELRDAIIHLPAAYRSAVVLHDVQGLTSAAVADIQDVSLAAAKQRISRARA
                     MLVTHLARGTERRAALGGIPLRCWDARSRVSDYLDGDLDSATRVALETHLGSCPTCPA
                     LYAGLVGVRAALGALRDPDTVVPPALAARLRAAEQASVAKDVAKDSGQARR"
     gene            3800..5107
                     /locus_tag="PLB86_09565"
     CDS             3800..5107
                     /locus_tag="PLB86_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRC65422.1"
                     /translation="MSQRIRAKPGGDMGEFRLDGLMLGVATAATQIEGGCDTTNWADW
                     ALTPGHIADGSSPVRATGHWDRWREDTDLMASLGIQTYRFGVEWARLEPQPGVFSDEA
                     FSHYRDELTALRDKGIRPLLTLHHFNNPRWFEALGAFERADNVAHFLRFVERVVTELG
                     DLVEDWCTINEPNVYATFGYLLGRFPPGVRSLPRAVRVMTNLAHAHITAYELIHALRA
                     GQPTRVTFAVHLRVFDPANPANPAHRALARAQEYLFQTALTEAMCTGRFGMPLRRPAG
                     IHEGRYYDALGINYYSRSWVSTIGDGTRPGAPLNDLGWEIYPEGLARVARWAYDRWPA
                     PIWVTENGTCDATDRFRAQYLHDHLEVMAAARATGLPFERYYHWCFVDNWEWAEGEVP
                     RFGIVDLDYATLERTVKSSGRFYADVIAEGGVTDEIAARYLSS"
     gene            complement(5092..6687)
                     /locus_tag="PLB86_09570"
     CDS             complement(5092..6687)
                     /locus_tag="PLB86_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC65423.1"
                     /translation="MPVESAGPSAGADSGADLDRCPQVLLRNVRVVPVGGVPAPQGSV
                     DVRMRAGRVVDLAPTVTPDPRDSEVHDGEGRWIIPGLWDHHVHLAQWADTLSRLDLSG
                     TTSPDEVTDRIRRHLDTLPANDVDSVVVGFGHRSATWARQPTVAELDAVSGRHPVALV
                     SGDGHNGWLNSAALALLGAPATEGALVENDWYPVWTRIGSLPGSQEARDAAYRLAVSR
                     AAALGIVGVGDMEFGDGFLDWPRRFAAGVQDLRVRVATYPTGLDAVLAAGLRSGDVLG
                     DSGGLVRMGPLKIISDGSLNTRTAYCHEEYAGGSGLAEPRGRANYSGADLAELARRAT
                     QAGLRMAVHAIGDAAITQALDVFESTGARGTIEHAQLMQPSDVVRMARLGVGASVQPA
                     HLLDDRDVTALCWPDRADRCFPLREMVEAGVALLLGSDAPVSPLDPWLAMAAAVHRSA
                     DERDPWNAGQSLTAAQALAGSTDGCTTVTLGSRADLVVLEADPLDGAAGDSAAVGQRL
                     RTMPVAATYLGGRRTFGSGHDDR"
BASE COUNT          978 a         2514 c         2246 g         1006 t
ORIGIN      
        1 tggagcaggt gcggctgtcc tggcacgcct ccgaggacgg cccgcgttcg ctcgtcgacc
       61 tgcacctggc gccccagggc gagcagacct acgtgtcgat tcgccacgag cacgtcgagg
      121 gcgacctcga ctcgctcaag gcccgctggg cagcggcgct gagccggctg gaagccgccg
      181 caggctgagt agtcccggcg cgatgcgtcg cggggccggg tcaacgggcc acgcgacgcg
      241 tcgcgaccgt gtgtcgagag cgtttcccgt gacgctgagg cgcaatcgcg gcacactcgc
      301 ctggtgaccg ccgcaccagc ctcgcctgag ccatcgctcc aggaaccgcc gccccacgcg
      361 gggctccaac ggtggcttcc gatcctcacc tggctgccga cctacgagcg ccgctggatc
      421 aaggacgacc tcctggcggg gttggggctc accgccctcc tcgtgccgca aggcatggcc
      481 tacgcgcagc tcgcgggcct gccgccgatc accggcctct acacgtcgat catgtgcctc
      541 gttgcgtatg ccgtgttcgg cccctcccgc gtcctcgtcc tcggcccgga ctcctccctc
      601 ggcccgatga ttgccgcgtc gatcctcccc ctggtcgcgg ccaacggtga ccccatgcgg
      661 gcggtcgtcc tcgcctcggc ccagtcggtc ctcgtcggca tcttcatgat cgcggccggt
      721 gccgccaagc tcgggttcgt cgcggacctg ctgtccaaac cgacgatggt cggctacatg
      781 aacgggctcg cgctcacgat cctcgtcggc caggtccgca agttgtgcgg cttctccatc
      841 tcggccgacg ggttgatcgg cgagatcgtc gcgaccgtcg agaagatcct cgctggggcg
      901 accaacctct gggcgcttgg catcggcgtc gtggccctgg tcatcatgag ggtcctgcag
      961 aagaccctgc cgcaggtgcc ggccgtcctc gtggccgtca tcgtcggcat cggcgcctcg
     1021 acctacctcg acctgcccgg caagggggtg aaggtcgtcg gtgacctgcc gcagggcttc
     1081 ccacccttca ccctgcccct ggtctcggtc ggggacatcg gcctgctcgt cgttggcgcc
     1141 ctcggcatca cgctggtggc ccttgccgac acgatctcga cggcctcggc atacgcccga
     1201 cggaccaagc aacccgtgga cggcaaccag gagatggtgg ggatcggcgc cgcaaacgtc
     1261 ctcgcggggc tcttccaggg gttcgccgtg agcacgagcg gctcgcggac ggctgttgcc
     1321 ttcaaggcag gcgcccgcag ccaggtcacc ggcgtcgtcg gcgctctcgc gatcctgctg
     1381 ctcctggtgg tcgcccccaa cctgctctcg aacctgccac aacccgtcct gggagccatt
     1441 gtcatcctcg ccgccttctc gctggccgag gtccccgctg cccgcaagct ctggtcacag
     1501 cggcgaacgg agttctggct gtcgatcgcc gcgaccctgg ccgtggccgt catgggcgtc
     1561 ctccccggca tcggggtcgc cgtcgggctg tcgatcctca acgtgttccg ccgggcctgg
     1621 tggccgtatc aggcggtgct cggccgggag aagggccacc cgggctacca cgacatgacg
     1681 catcggccgc ggctcacgtc gatgccgggg ctcatcatct tccgcttcga cgcaccgctg
     1741 ttcttcgcca acgcccggac cttcggggag cagatcaccc gactggccgc gaccgaaccg
     1801 gcacccaaat ggatcctcgt cgcagccgag ccgatctccg acatcgacac aacggccgcc
     1861 gacatgctcg ccagcctggt tgccgatctg gacgagcgcg ggacgaaact ggccttcgcc
     1921 gagctcaaga gcgtcgtcag gggcaagctc gaggcctacg cgctcgacgg ggtcatcgac
     1981 aacgttctct acttccccac cctcaacgcg gcggggaagg cctatcgggc gcagttcggg
     2041 accgactggg gcgacacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc
     2161 ggcaccgacc aggcggtgta tgccgtcgcg cgcgacgagc ttgcgcactg ggctgccgag
     2221 gtgggtcggg acctcccgcc cggagcgttc ggcgagaacc tcaccatcac tggcctcgat
     2281 gtcgacggtg ccctcgtcgg ggagatctgg gcgatcggtg acgtcgtccg cctgcaggtg
     2341 accgggccgc gcatcccgtg cgcgaccttc gccagggcca tgggggagga acgctgggtc
     2401 aagcgcttca cggtgcgcgg ccgcaccggc gcctacctgc gcgtcctcac tccgggcccg
     2461 gtgcaggccg gcgacgcggt cgtcgtcgag agccgacccg accacgagat caccgtgccg
     2521 ctgatgttcc gggcgctgac gaccgagcgg cacctcttgc cgcggctcag cgaggtcggt
     2581 cccgacctga gcgaggaggg ccgccgggag ctcgcggcct accgcgaggg gaccaccgac
     2641 ggccggatgc cgtagcgcat cgagtgtgac ccacgacata cccggttcct gtcacctttg
     2701 cgcggtccgc accgtccatg cggcgttcga ccctctgacc taaggaaacg acgcaccatg
     2761 aagctcaacg tcggcaccac cgaccggaag atccgcattg ccatcgccat cgtcgccgcg
     2821 atcctcgcgg tcgtcgtcgg cggcaccttc ggggtggtcc tgtgggtcgt cgcggcgatc
     2881 atgctcgcca ccggcatcgt ccgcgtgtgc ccgctctacc tccccttcaa gatcagcacc
     2941 aacaagggcg cctgacaccc tccggtatgc cgtccgcccg cctcccgctc accgcggggg
     3001 gaccggacgg cataccgttg tcgcatgggg atcacgcacg aggagatcac ggccgccgtc
     3061 cctggcctgt tgcgctacgc gcgcgtcctg acccgggatg ccgcgacggc cgaggacctc
     3121 gtgcaggaca ccgtggtgcg cgccctggag cgcgcggccg acttccgtgg tgactcctcc
     3181 gccgcaacct ggctgcaccg gatcctgcac caccggttcg tcgacgtcgt ccgggcctcg
     3241 cgctccgacc ccgtcgatgc cgaagaactc gccgaacgcg tcgaggccga ctggcgcgac
     3301 gattcctaca ccgtcgacgc cgagaccgtt ctcctgcggg ctgagaccgc cgaggaactg
     3361 cgcgacgcga tcatccacct tccggcggcc taccgctcgg ccgtcgtcct gcacgacgtc
     3421 cagggcctga cctcggcggc ggtcgccgac attcaggacg tcagtctggc cgccgccaaa
     3481 caacggatca gccgggctcg ggcgatgctc gtcacccacc tcgcacgcgg caccgaacgg
     3541 agggcagcct tgggcggaat tcccttgcgc tgctgggacg cccgctcacg ggtgagtgac
     3601 tacctcgacg gcgacctcga ctcggccacg cgcgtcgcgc tcgaaacgca cctcggctcg
     3661 tgccccacgt gccccgccct gtatgccggt ctcgtcggcg tgcgcgccgc actcggcgcc
     3721 ctgcgcgacc ccgacacggt cgtccccccg gccctcgccg cacggctccg ggcggccgag
     3781 caggccagcg tcgcaaagga tgtcgcaaag gattcgggcc aagcccggcg gtgacatggg
     3841 cgagttccgg ctcgacggcc tgatgctcgg cgtcgccacc gcggccaccc agattgaggg
     3901 tggctgcgac acgacgaact gggccgactg ggcgttgact cccgggcaca tcgccgacgg
     3961 atcctcgccc gtccgggcca cggggcactg ggatcggtgg cgcgaggaca ccgatctgat
     4021 ggcctcactc ggcatccaga cctaccgttt cggggtcgag tgggctcggc tggaacccca
     4081 gccgggcgtc ttctccgacg aggccttcag ccactatcgc gacgagctga ctgccttgcg
     4141 ggacaaggga atccggccac tgctgacgtt gcatcacttc aacaaccccc gatggttcga
     4201 ggcgttggga gccttcgaac gagccgacaa cgtggcgcac ttcctgcgct tcgtcgagcg
     4261 ggtcgtcacc gagctcggcg acctcgtcga ggactggtgc acgatcaacg aacccaacgt
     4321 ctatgcgacg ttcggctacc tcctcgggcg gttccccccg ggcgtccggt cgctgccgcg
     4381 agccgtgcgg gtgatgacca acctcgcgca cgcccacatc acggcatacg agctcatcca
     4441 cgcgttgcgc gcgggtcagc cgacgcgcgt caccttcgcg gtccatctgc gcgtcttcga
     4501 ccccgccaac ccggccaacc ccgcacaccg cgccctcgcg cgggctcagg aatacctctt
     4561 ccagacggcc ctcaccgagg cgatgtgcac gggccgattc ggtatgccgc tgcgccgccc
     4621 cgcggggatc cacgaggggc gctattacga cgcgctcggg atcaactact actcgcgctc
     4681 ctgggtgagc acgatcggcg acgggactcg gccgggggcg ccgctcaacg acttggggtg
     4741 ggagatctac cccgaggggc tggcgcgcgt ggcgcggtgg gcctatgacc ggtggccggc
     4801 cccgatctgg gtcaccgaga acggcacctg cgacgccacc gaccgcttcc gtgcccaata
     4861 cctgcacgat cacctcgagg tgatggccgc ggcgcgcgcg accggcctgc cgttcgagcg
     4921 ctactaccac tggtgcttcg tcgacaactg ggagtgggcc gaaggcgagg tgccgaggtt
     4981 cgggatcgtg gacctggact acgccaccct ggagcgcaca gtgaagtcga gcggacgctt
     5041 ctatgccgac gtcatcgccg agggcggcgt caccgacgag atcgccgcgc gctacctgtc
     5101 gtcgtgaccc gagccgaacg tccgcctgcc gccgaggtag gtggcggcca ccggcatcgt
     5161 ccgcagccgc tgccccaccg cggctgagtc gccagccgct ccgtccaacg gatcagcttc
     5221 cagcacaacg agatccgcac ggctgcccag cgtcacggtg gtgcagccgt cggtgctgcc
     5281 ggccagcgcc tgcgccgcag tgagagactg cccggcgttc cacgggtcac gctcgtccgc
     5341 gctgcggtgc acagccgccg ccatcgccag ccacgggtcc agcggcgaca ccggcgcgtc
     5401 ggagccgagc agcagcgcga cgccggcctc gaccatctca cgcaggggga agcaccgatc
     5461 ggctcgatcg ggccaacaca acgcggtgac gtcgcggtcg tccagcagat gggccggctg
     5521 cacactggcc cccacgccga ggcgagccat ccgcacgaca tccgacggct gcatcaactg
     5581 cgcgtgctcg atggtcccgc gggcgcccgt cgactcgaac acgtccaacg cctgcgtgat
     5641 cgccgcgtca ccgatggcgt gcaccgccat ccgcaggccc gcctgggtcg ctcgccgggc
     5701 caactcggcg aggtccgcac cggagtagtt cgcgcgacca cgcggctccg cgagaccgga
     5761 cccaccggca tactcctcgt ggcagtaggc cgtgcgggtg ttgagcgagc cgtccgagat
     5821 gatcttcagc ggtcccatcc gcaccagccc gcccgagtcg ccaaggacgt cgccgctgcg
     5881 caggccggct gcgagcaccg cgtccagccc ggtggggtag gtcgccacac ggacccgcag
     5941 gtcttgaacc ccggccgcga atcgccttgg ccagtcgagg aatccgtcgc cgaactccat
     6001 atcaccgacg ccgacgatcc ccagcgcggc ggcacgcgac accgcgagtc ggtatgccgc
     6061 gtccctcgcc tcctgcgacc ccggcaggct gccgatccgg gtccacacgg gataccagtc
     6121 gttctcgacg agggcccctt cggtcgccgg tgcgccgagc agggcgagcg ccgccgagtt
     6181 gagccacccg ttgtgtccgt caccgctgac gagagcgacc gggtgccgcc cggacacggc
     6241 gtcgagctcg gccacagtgg gctggcgggc ccaggtcgcc gaccgatgac cgaagccgac
     6301 gaccacactg tccacgtcat tcgctggcag tgtgtcgagg tgtcggcgga tccggtcggt
     6361 gacctcgtcg gggctcgtcg tgcccgacag gtcgagacgc gagagggtgt cggcccactg
     6421 ggccaggtgg acgtggtgat cccacagccc agggatgatc caccggccct cgccgtcgtg
     6481 gacctcgctg tcgcgcgggt ccggcgtgac cgtcggggcc aggtcgacga cgcgaccggc
     6541 gcgcatccgc acgtcgacgc tcccctgcgg agccggcacc ccgccgaccg gaacgacccg
     6601 cacgttgcgc aggagcacct gggggcagcg gtcgaggtcg gcaccggagt cagccccggc
     6661 gctcggcccg gcggactcga caggcatgcg gaggcctttc gcgcggggag cagacgccct
     6721 cacgctagcg cccgaccccg ccgctcgcct cgccctgcgg gggcaccgag acgcccatgg
     6781 gagacttggg cgcaggggaa ccagccgcgc agggaggatg tcgatgtcgt cgccgatcac
     6841 cagg
//
LOCUS       DAORPW010000122         6841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2902_length_6841_cov_818.236369, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000122 DAORPW010000000
VERSION     DAORPW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2902_length_6841_cov_818.236369"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..2395
                     /locus_tag="PLB86_09575"
     CDS             140..2395
                     /locus_tag="PLB86_09575"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRC65424.1"
                     /translation="MTTGIVLVALAVGIAMNALWVPAPSSGLADTYGWGLPAFARGEW
                     WTLLVGAFIAPAPWMYLLILPLIAVGGGFLEYKYGAWRMLAALFVTHVASVLIVGLII
                     YLLAPLGVGWAVRLVDVRDGGLSNAGFGAIGAMTAALAPSWRRRARTGLFLYALVMVL
                     YVGFIWDLTHLVALLVGFAVGPLVVKRPYARFSYALTTQEKRVLVAQIVVFNIITLVV
                     TYLVPQGGLLESSDTYQRDAGSTVVVFGLSIFLGLLAYGTYAGRKIAWRILLTLTILL
                     FLIGLILAETPAGVFGVFVNAVLLVLLVVFRRVFTIKGDRVVRSGIYKRLAALALGLF
                     MLNAVAIFAMRAQFSPEPDFGMAVVESIWLIFGASSLDFVPQTPAASFLTQGIGYVWG
                     FALLGGIGALVFTTLRDRDGRGQFAEYDRLLHEHGSTSIGWMARWPGMSYWVDPARTA
                     GFAYRLENNIAIVLSDPVGSPDAVATSLGGFTEMCDRRGWRPVFYSVTESFRALAAQH
                     GYQAIVVGEDTVIDLPDLAFTGKSWQSVRTAVNQAGKLGISMRPIRYAEATAGMRVQL
                     QAIAASWSDDKSLPPLGFTLGTLAEASDPEVIMHVAVDAEGTVHGMTSWMPVYGQGTV
                     DGWTLDIMQRGLFEDTMRGVVEFLIAESAMAFKQEGYRFISLSASPLSRGEGQTGPIE
                     ALLDMVAKRLEPLYGFQSLHSFKAKFKPRFEPMYLCYRDEAQLPAISIAIIKAYLPDN
                     ALRGVVASKIK"
     gene            complement(2473..3879)
                     /locus_tag="PLB86_09580"
     CDS             complement(2473..3879)
                     /locus_tag="PLB86_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC65425.1"
                     /translation="MTAGPRWFQRLSIRWRLMTVGLVGLTVALVAAGSLLYAVLSATL
                     ANTLGAEANSAAREVATLVEQGRLSDPVPVSGALVVQVLDSSQRVVAGSLVADRLTPL
                     LTADEVRRTLAGEQLTVPGSRSGLSGRLHVAAVSAGPGSAPVTVIAGVPTGEVDVSLG
                     LVRTLLFVGLPLLLLALAAVAWRVIGAALAPVEALRLGAERIGADARPRRRRATVDRL
                     PIPVADDEIHSLATTLNGMLDRLDGLAERQQAFLDDAAHELRSPLASLRMQLEVAEHV
                     GDGGSLPADLLPDVERLSRLVDDLLVLARSGNGEAAPSLETVDLRPLLREIVSRYAAA
                     RVPVTLTDVAPGATAIEVPGNRADLRRVVTNLVDNAVRHASTAVTLSVGADPSGTWLR
                     VTDDGHGIPLADRERVFERFTRLDEARDRDSGGTGLGLPIARELMRRNGADVTLTEAA
                     PGVIAVVAWPRSTEKPSS"
     gene            complement(3876..4529)
                     /locus_tag="PLB86_09585"
     CDS             complement(3876..4529)
                     /locus_tag="PLB86_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC65426.1"
                     /translation="MRVLVVEDERGMAAALHRGLSAEGFVVDVCRDGPSGLEAARFGG
                     YDAVLLDLMLPGMSGYTVVRTLREEANWVPVIVLSAKDGEYDQADALDYGADDYLTKP
                     FSFVVLLARLRALVRRDPTPRPPTVSAHGIRLDPATREVTVDGVAVELALREFAVLER
                     LLTAAPAVVSKEELLDAVWGDAAEGINVVEVYVGYLRRKLGRERIETVRGVGYRIAP"
     gene            4635..>5510
                     /locus_tag="PLB86_09590"
     CDS             4635..>5510
                     /locus_tag="PLB86_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65427.1"
                     /translation="MTASPLRSRFGRWLVPAAAVVLVAGVSLAGSRMASADSDLTPRT
                     AAELLADLQRARLDHLSGTVVQTADLGLPALPGVSGMSGNVGAGASELTSIISGSHTW
                     KVWYGGENQQRLALVGTLGESDIIHNGSDVWIWSSKAQTATHVVIDPAKAAAAKSEAG
                     KTGGPLMTAGPTGTGLPTTPEDAAKAALAAIDPTTRAEVGPAVVVAGRTAYELVLTPK
                     DSTTLVAQVRLAVDGERHLPLRVQVFSTKQATPAIEVGFTSIDFSAPEARQFEFTPPP
                     GTTVTEGMPEGLGDKA"
     assembly_gap    5511..5610
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5611..5877
                     /locus_tag="PLB86_09595"
     CDS             <5611..5877
                     /locus_tag="PLB86_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65428.1"
                     /translation="GTGWSRVVVATMPATTDAAPGGGTAADGGLGQLQSLLGALPQVS
                     GAWGSGRVLEGTLFSLVLADDGRVALGAVAPDALYAALAAPAAG"
     gene            5888..6817
                     /locus_tag="PLB86_09600"
     CDS             5888..6817
                     /locus_tag="PLB86_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC65429.1"
                     /translation="MLAVQTWELTKRFGSQTVVDGIDLAVPAGSVYGFLGPNGSGKTT
                     TIRMLLGLIGPSAGRSELLGLPVPGQAEAALHRVGALVEGPAFHPYLSGRANLARLDV
                     ADRRADPRTSRARVDAALDRVGLLAAAEKRYRAYSLGMRQRLAIAGALLMPRELLVLD
                     EPTNGLDPQGTREVRALIGSLGSEGTTVLVSSHLLSEVAQVCSHLGVMREGRLVAQGP
                     ITELQGAATRATVRTDDGEQAAYVLRELGATGVSVDAEHAQVRGELGAIAPEKAVAAL
                     VHAGVAVRGFSSGAPDLEDWFVGLTGEGFDVSG"
BASE COUNT          977 a         2316 c         2389 g         1059 t
ORIGIN      
        1 cctgggaagg tgactccgtt gcccgacgta gctgtgcgac aatcttccgc gcccatccca
       61 aggctcgagg cgtacggcac tgaaggaatc catgaggacg gtactccggg aaacgctgtg
      121 gtggatcaga aaggcgcccg tgacaacggg aatcgtcctg gtcgcgctcg ctgtcggcat
      181 cgctatgaac gcactgtggg tgcccgcccc ttcgtccggc ctggcggata cctacggctg
      241 gggtctgcct gcctttgccc gcggtgaatg gtggacgctt ctggtggggg cgttcatcgc
      301 accggcgcct tggatgtacc tgctgatcct gccgctcatc gccgtcggcg gcggcttcct
      361 tgagtacaag tacggcgcct ggcggatgct cgccgccctc ttcgtcacgc acgtcgcctc
      421 ggtgctgatc gtcggcctga tcatctacct gctggcgccc ctgggcgtcg ggtgggcggt
      481 caggctggtg gacgtccgag acggcgggct ctccaacgcc ggcttcgggg cgattggtgc
      541 catgaccgcg gccctcgccc cgagttggcg gcgacgtgct cgcaccggcc tgttcctgta
      601 cgccctggtc atggtgctgt acgtcgggtt catctgggat ctgacccact tggttgcgtt
      661 gctggtcggc ttcgctgtcg gcccgctggt ggtcaaacgc ccctacgcga ggttcagcta
      721 cgcgctgacg acccaggaga agcgcgtcct ggtcgcccag atcgtggtct tcaacatcat
      781 caccctggtg gtcacgtacc tcgtgccaca aggtggcctg ttggagtcga gcgacacgta
      841 ccagcgggac gcgggcagca ccgtcgtggt gttcggtctc tcgattttcc tgggtctgct
      901 ggcctacgga acgtacgcgg gccgcaagat cgcctggcgg attctgctga cgctgacgat
      961 tctgctcttc ctcatcggct taatccttgc ggagactccg gcgggggtct tcggcgtctt
     1021 cgtcaacgca gtcctcctcg ttctcctcgt cgtcttccgg cgcgtcttca cgatcaaggg
     1081 cgaccgggtc gtccgatcgg gcatctacaa gcgtctcgcg gcgctcgccc tcggcctgtt
     1141 catgcttaac gcggtggcca tcttcgccat gcgcgcacag ttctcgcccg agccggactt
     1201 cggcatggcg gtcgtcgagt cgatctggct gatcttcggc gccagctctc tcgacttcgt
     1261 cccacagacg ccggccgcga gcttcctgac ccagggcatc ggatatgtct ggggtttcgc
     1321 gctgctcgga ggaatcggag cgctggtgtt caccaccctg cgcgacaggg acgggcgtgg
     1381 ccagttcgct gagtacgacc gccttctcca cgagcatggc agcacctcga tcggctggat
     1441 ggcgcgttgg cccggcatga gctactgggt ggacccagcc aggaccgctg gcttcgccta
     1501 ccggttggag aacaacatcg cgatcgtcct gagcgatccc gtcggcagcc cggacgccgt
     1561 ggccacgagc ctgggcggct tcaccgagat gtgtgacagg cgcggttggc gtccggtgtt
     1621 ctacagcgtg accgagtcct tccgcgcgct ggcggcgcag cacggctacc aggcgatcgt
     1681 ggtcggcgag gataccgtca tcgatctccc cgacctggcg ttcaccggaa agtcgtggca
     1741 gagcgtccgg acggccgtga atcaggcggg caagctcggg atcagcatgc gtcccatcag
     1801 gtacgccgag gccaccgccg ggatgcgcgt tcagctccag gcgatcgccg cgagttggtc
     1861 cgacgacaag tcgctgcccc cgctgggctt caccctgggc acgctggcgg aggcgtccga
     1921 tcctgaggtg atcatgcacg tcgccgtcga cgccgagggc accgtgcacg ggatgaccag
     1981 ctggatgccc gtctacggcc agggcacagt ggacggctgg acgctcgaca tcatgcagcg
     2041 cggcctgttc gaggacacga tgcgtggcgt cgtcgagttc ctgatcgccg agagcgcgat
     2101 ggcgttcaag caggagggtt accggttcat cagcctgtcg gcgtcgccgc tgagtcgggg
     2161 tgaggggcag accggcccga tcgaggcgct gctcgacatg gtggccaagc ggctggagcc
     2221 gctctacggg ttccagagcc tgcacagctt caaagccaag ttcaagccgc ggttcgagcc
     2281 gatgtacctg tgctaccggg acgaagccca gcttccggct atctcgatcg ccatcatcaa
     2341 ggcctacttg cccgacaacg cactccgcgg cgtcgttgcc tcgaagatca agtaggacgg
     2401 cagcgcgctc aggaggtggg cggcaccgct gggagccggc tgccgagtcc gtctggtcca
     2461 gctcgcggtc ggttagcttg agggcttttc ggttgaccgc ggccaggcca cgacggcgat
     2521 cacgccgggt gctgcctcgg tcagggtgac gtcggcgccg ttgcggcgca tcaactctcg
     2581 cgcgatcggc agacccaacc cggtgccgcc gctgtctcga tcgcgggctt cgtcaaggcg
     2641 cgtgaacctc tcgaagacgc gctcccggtc agccaggggt atgccgtgcc cgtcgtctgt
     2701 cacgcgcagc caggtcccgc tcgggtcggc tccaaccgag agggtcaccg ccgtcgaggc
     2761 gtggcggacg gcgttgtcga cgaggttggt caccacccgc cgcaggtcgg cccgattgcc
     2821 gggaacctcg atggccgtgg cgcccggcgc gacatcggtg agggtcacgg ggactcgggc
     2881 ggcggcatac cggctgacga tctcgcgcag cagggggcgc aggtcgacgg tttccaggct
     2941 cggcgcggcc tcgccgttgc cggagcgcgc caacacgagc aggtcatcga cgagccggct
     3001 caaccgttcg acgtcgggca gcaggtcagc cggcagcgag ccgccgtccc cgacgtgctc
     3061 ggccacctcc agctgcatgc gcagggaggc cagcgggcta cgcaactcgt gggccgcgtc
     3121 gtcgaggaag gcctgctggc gctcggcgag cccgtccagt cgatcaagca tgccgttgag
     3181 agtcgttgcc agagagtgga tctcgtcatc agcgacgggg atcggcaggc ggtcgaccgt
     3241 cgcacggcgc cgacggggcc gagcgtcggc gccgatccgc tcggccccga ggcgcagcgc
     3301 ctcgacgggg gccagggcgg caccgatgac ccgccaggcg acggcggcga gcgcaagcag
     3361 cagcaggggc agcccgacaa agagcagcgt tcggaccagg ccgaggctca cgtcgacctc
     3421 accggtgggc acgccggcga tgacggtcac cggcgcagac cccgggccgg ccgacaccgc
     3481 cgcaacgtgc agtcgccccg agagaccgga gcgcgagcca ggcaccgtga gttgctcgcc
     3541 ggccagggtg cgccgcacct cgtcggcggt cagcaggggc gtgagccggt cggcgaccag
     3601 cgacccggcg acgacccgct gagaggagtc caacacctgc acgacgagcg cccctgacac
     3661 gggcaccggg tcagacaatc ggccctgctc gacgagggtg gcgacctcac gagccgctga
     3721 attcgcttcc gcgccaaggg tgttggcgag ggtggcggac agcacggcat acaggagact
     3781 gccggcggcc acgagcgcga cggtgaggcc gacgagcccg acggtcatga gccgccagcg
     3841 gatggacagc cgctggaacc agcgcggccc ggccgtcatg gcgcgatccg atagccgacg
     3901 ccgcgcaccg tttcgatccg ctcgcgcccg agtttgcggc gcaggtagcc gacgtagacc
     3961 tcgacgacgt tgatgccctc ggccgcgtcg ccccacaccg cgtcgagcag ctcctccttg
     4021 ctcaccacag ccggggcggc cgtcaacagc cgctccagga cggcgaattc gcgcagcgcg
     4081 agctcaacag ccaccccgtc gacggtcacc tcacgcgtcg ccggatccag ccgtatgccg
     4141 tgcgccgaca ccgtgggcgg gcgcggcgtc ggatcccgcc ggaccaaggc ccgcagccgg
     4201 gcgagcagga cgacgaagga gaacggtttg gtgaggtagt cgtcggcgcc gtagtcgagg
     4261 gcgtcggcct ggtcgtattc gccgtctttc gccgacagga cgatgaccgg cacccagttg
     4321 gcctcctcac gcagggtccg caccacggtg tagcccgaca tgcccggcag catgaggtcc
     4381 agcagcaccg cgtcgtagcc cccgaaccga gcggcctcca gaccggaggg gccgtcgcgg
     4441 catacatcga cgacgaagcc ctccgcgctc aggccgcggt gcagcgcagc ggccatcccg
     4501 cgctcgtctt cgaccaccaa cacccgcaca aggacacgat gacacgtgca cagcggcgga
     4561 tcgtctgcgc cgggcccgag ccgccggtat gccgtctcag ggtcctctca ggtggcgtcg
     4621 ggcatcctgg gcccatgacc gcttccccgc tgcgttcccg tttcggccgt tggctcgtgc
     4681 ccgccgccgc cgtcgtcctc gtcgccggcg tctcgctggc cggtagccgg atggcgtcgg
     4741 ccgacagcga cctgacccca cgcacggccg ccgagttgct cgccgatctg cagcgggctc
     4801 gcctcgacca cctgtcgggg accgtcgtgc agacggccga cctcgggctg ccggccctgc
     4861 ctggcgtgag cggcatgtcg gggaacgtcg gtgccggtgc gtccgagctg acctcgatca
     4921 tcagcggcag ccacacctgg aaggtctggt acggcggcga gaaccagcag cgcctggccc
     4981 tggtcggcac gctgggcgag tcggacatca tccacaacgg cagcgacgtg tggatctggt
     5041 cgagcaaggc ccagaccgcc acgcacgtgg tcatcgaccc cgcgaaggcc gccgcagcca
     5101 agagtgaggc cgggaagacc ggcgggccgt tgatgacggc cgggccgacg ggcaccgggc
     5161 tgcccaccac gcctgaggac gccgccaagg cggcgctcgc ggccatcgac ccgacgaccc
     5221 gggccgaggt cgggcccgcg gtggtcgtcg cggggcgcac ggcatacgag ctggtgctca
     5281 cgcccaagga ctcgaccacc ctcgtcgccc aggtgcgcct ggccgtcgac ggcgaacgac
     5341 acctgccctt gcgggtgcag gtcttctcga ccaagcaggc cacgccggct atcgaggtcg
     5401 ggttcacgtc gatcgacttc tcggcgccgg aggctcggca gttcgagttc accccgccgc
     5461 ccgggacgac ggtgactgaa gggatgccgg aaggcttggg agacaaggct nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcaccggct ggtcgcgggt cgtcgtggcc
     5641 acgatgcctg ccaccacgga cgcagcgccg ggcgggggaa ccgccgccga cggtgggctg
     5701 ggtcaactgc agagcctcct gggcgcgctg ccgcaggtct cgggtgcgtg gggcagtggc
     5761 cgggtccttg aagggacgct gttctcgctc gtcctcgccg acgacggccg cgtcgcgctc
     5821 ggggcggttg cccccgacgc cctgtatgcc gccctcgccg cgcccgcggc cggctgacgg
     5881 cgcgcggatg ctggcagttc agacgtggga gttgaccaaa cggttcggct ctcagacggt
     5941 ggtcgacggc attgacctcg cggtgcctgc cgggtcggtc tatgggttcc tcggccccaa
     6001 cgggtcgggc aagacgacga cgatccggat gctgcttggg ctgatcggcc catcggctgg
     6061 gcgctcggag ctgctcgggc tgccggtgcc cggccaggcc gaggcggcgt tgcaccgggt
     6121 cggcgccctg gtcgaagggc cggccttcca cccctacctg tccggtcggg ccaacctggc
     6181 ccgccttgat gtcgccgacc gccgggccga cccgcgcacg tcccgtgcgc gggtcgacgc
     6241 ggcgctcgat cgagtcggcc tgctcgccgc cgcagagaag cgctaccggg cctattcgct
     6301 cggcatgcgc cagcggctgg ccatcgcggg cgccctgctc atgccgcgcg agctgctcgt
     6361 cctcgacgaa cccaccaacg ggctggaccc ccaaggcacc cgtgaggtgc gggccctcat
     6421 cgggtcgctc ggcagcgaag gtacgacggt gctcgtctcc agccacctgc tctcagaggt
     6481 cgcccaggtc tgctcgcatc tcggtgtgat gcgcgaaggc cggctggtcg cccaaggccc
     6541 gatcaccgag ttgcagggcg cggccacccg ggcgacggtg cggaccgacg acggtgagca
     6601 ggcggcatac gtgctgcgtg agctcggcgc gacgggggtg agcgtcgatg cggagcatgc
     6661 tcaggtgcga ggtgagctcg gcgcgatcgc cccggagaag gcagtggcgg cgctcgtcca
     6721 cgcgggggtc gcggtccgcg gattctccag tggggcaccg gatctcgagg attggttcgt
     6781 tggcctgaca ggggagggtt ttgatgtcag cggctgaggt gtcgacggct ggcgcggtga
     6841 c
//
LOCUS       DAORPW010000123         6737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_2964_length_6737_cov_735.934002, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000123 DAORPW010000000
VERSION     DAORPW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2964_length_6737_cov_735.934002"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLB86_09605"
     CDS             complement(<1..269)
                     /locus_tag="PLB86_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65430.1"
                     /translation="MENNSGVSSSTPSQEPSAAEARRALEALAADRRELADRMATPTW
                     YHPALAALVFILVAGYGFERLFLIALVLYSAGLVALVTAYRRITGV"
     gene            complement(314..1375)
                     /locus_tag="PLB86_09610"
     CDS             complement(314..1375)
                     /locus_tag="PLB86_09610"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRC65431.1"
                     /translation="MSAPSLPTPYLRLDVAKAQASIEAMAARARALGVGLRPHAKTHK
                     SVRLGRLQLEAGAVGLTVATVGEAEVFAAGGCADLFIAYPLWVDAPKARRLAAVLEWA
                     SVAVGVDSTASLDAMAAHGIRPEVVVEVDSGHHRSGVSPEAAGEIGRYAADAGFVVRG
                     VFTFPGHSYTPQTRASAAADEAIALAVAAESLAGQGIAVSVRSGGSTPSAQFAVGSGG
                     GGLTELRPGVYLFGDAQQVELGTCAMEDVALTVVATVVSHAGGRVILDAGSKALGADR
                     APWASGYGRLAEAPDARIVALSEHHATVEWSGGRLPQLGSRVLVVPNHVCNTVNLADV
                     YVIGDGPEAWPVDARGCNS"
     gene            complement(1383..1958)
                     /locus_tag="PLB86_09615"
     CDS             complement(1383..1958)
                     /locus_tag="PLB86_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65432.1"
                     /translation="MDGPNLWIVLALGLVAIGWYLTYTAARLDRLHARVQGTLAALDA
                     QLVRRAEATLELANARVLDAASALMLASAASDSLSAAEDEDTDADRQRFSNERETVES
                     RLSEALGLALTADTLASLPGAGGFGDDIVSRVRATGLRVQLARRFHNDAVTDVRRVRR
                     QWAVKWFRLAGHADMPQTVEFVDALPHGLRG"
     gene            complement(1958..3139)
                     /locus_tag="PLB86_09620"
     CDS             complement(1958..3139)
                     /locus_tag="PLB86_09620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC65433.1"
                     /translation="MRIGIVCPYSFDIPGGVQFHVRDLAEILRSRGHTVSVLAPADEG
                     TELPDYVVSAGRAVPVSYNGSVARLNFGLMTNAKVTAWLDRGHFDLVHIHEPITPSIG
                     ALALWAADCPIVATFHTSNLRSRAMQVSYPFLRSSMEKILGRIAVSEDARRTLTTHFG
                     GDAVVIPNGVYVDRFASTLPRPQWQGQPGRPTIAFLGRMGEPRKGMPVLAAALPAVLA
                     QVPGLRLLVAGPGDADEVRAAMTPEVAAATEFLGAVDDAAKAALLRSVDVYIAPNTGG
                     ESFGIILIEAMSAGAAVLASDIPAFVRVLDGGTVGATFVNEDPDDLARQLVRLLRDDT
                     ERARLAAAGRARADLFDWSVVADAITLVYETVCEGAAAARAMPGHGVLGRLRRGGRSL
                     P"
     gene            complement(3146..4102)
                     /locus_tag="PLB86_09625"
     CDS             complement(3146..4102)
                     /locus_tag="PLB86_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HRC65434.1"
                     /translation="MGGLSGVLATARARGTVWGYRAGWSAVRRMPERAAYRVFDTVAD
                     ASVRRGGKGLARMRANYAKVRPELDDAALDALVREGMRSYTRYFCEAFRLPDCTHDDL
                     DRMCRAVNDAAVRANLDAGRSAVIFVPHMGNWDIAGAWSTGHLAPVTTVAERLEPEEV
                     FREFFEFRESLGMTILPLTGGPNPFNGLREAVERGDFVALVSDRDLTSNGVEVDFFGH
                     RARMAKGPALLALLTGAPLYAASIHFAPAAPGRGAAGRETVVTFSDEIPVPTEGDTAA
                     KAQAMTQACAAYVEGPIREHTSDWHMMQRVFVDDLDPTRLGR"
     gene            complement(4102..4761)
                     /locus_tag="PLB86_09630"
     CDS             complement(4102..4761)
                     /locus_tag="PLB86_09630"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRC65435.1"
                     /translation="MLNRLLRASWTKVMTPIAKAFLAIGLTPDAVTIIGTLAVCVSAL
                     AFYPRGEFLTGTLLITLFIFSDNVDGVMARLSGRQGVWGAFLDSTLDRVGDAAIFGGL
                     VLWFAGPGANWLMAGLALACLILGMVVSYAKARAEGLGMTCNVGIAERADRLFAVLLA
                     TGLVGLGLPLWVLTVVLALLSVASFITVIQRMATVHTQAMAALRADGAGTAGDSGPTE
                     T"
     gene            complement(4860..5426)
                     /locus_tag="PLB86_09635"
     CDS             complement(4860..5426)
                     /locus_tag="PLB86_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRC65436.1"
                     /translation="MTSDDPGRATADLESASDFARVEDGFERLWTPHRMAYIEGVRPT
                     EEAGDGCPFCAAPLRSDIEGLIVARGELCFVVMNLFPYNPGHLLICPYRHVPLYVDLT
                     DEETTEFTAMTKTALAALTRTSAPMGFNIGMNQGRVAGAGVAAHLHQHVVPRWGGDSN
                     FLPIIGQTKALPLLLEDVRTRLAEVWPA"
     gene            complement(5478..>6737)
                     /gene="thrS"
                     /locus_tag="PLB86_09640"
     CDS             complement(5478..>6737)
                     /gene="thrS"
                     /locus_tag="PLB86_09640"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRC65437.1"
                     /translation="DLTAYLDRLAEAERRDHRKLGIELDLYSFPDELGSGLPVFHPKG
                     GVLKREMEDYARRRHIEEGFLYVGTPHISKEGLFHTSGHLPYYADTMFPPMSMEGSDY
                     YLKAMNCPMHNLIYRSRGRSYRELPLRLFEFGSVYRYEKSGVIHGLTRVRGLEMDDSH
                     SYVTPEQAPAEIKHLLNFVLSLLRDFGLDDFYLELSTRDDSGAKKGKFIGSEEQWAEA
                     TQILADVAAESGLDLVPDPGGAAFYGPKISVQARDAIGRTWQMSTIQYDFNQPERFGL
                     EYQAADGSRQQPVMIHSAKFGSIERFMGVLVEHYAGAFPPWLSPVQVLGVPVAEEYAG
                     YLREVLGGLAAQGVRVELDESDDRFPKKIRNASKSKVPFVLIAGEDDRAAGAVSFRFR
                     DGSQRNGIPVAEAAAEILAAIASRTQV"
BASE COUNT         1112 a         2336 c         2354 g          935 t
ORIGIN      
        1 acaccggtga tccgccggta tgccgtcacg agagcgacca ggcccgccga atagagcacc
       61 aaagcgatca ggaacaaccg ctcgaagcca tagccggcca cgaggatgaa gaccagcgca
      121 gccagggccg ggtggtacca ggtgggcgtg gccatgcggt cggccaactc ccgacgatcg
      181 gcggcgagcg cctccaaggc ccggcgggct tccgctgcgg acggctcctg tgacggtgtc
      241 gatgacgaca cacccgagtt gttttccata ccggaaaact agcacaaggt ttccggaacg
      301 gaaagcccca gggttacgag ttgcagcccc gcgcgtcgac cggccaggcc tccgggccgt
      361 caccgatgac gtagacgtcg gcaaggttca ccgtgttgca cacgtggttg ggcacgacca
      421 gcacccgcga gcccaactgc ggcagtcgcc cgccggacca ctcgacggtg gcgtggtgct
      481 cggacaaagc gacgattcga gcgtcgggag cctccgccaa ccggccataa ccgctggccc
      541 acggcgcccg atcggcaccg agcgccttgc tgcccgcatc gaggatcacc cggccgccgg
      601 cgtgcgagac gaccgtcgcg acaacggtga gcgcgacgtc ctccatcgcg caggtgccca
      661 actcgacctg ttgggcgtca ccgaagaggt agaccccggg cctcagctcg gtgagtcctc
      721 ccccaccact tcccacggcg aactgcgccg agggcgtcga accgccactc cgcactgaca
      781 ccgcaatccc ttggcccgca agggattccg cggcgacggc cagagcgatc gcctcgtctg
      841 ccgccgccga tgcccgggtc tgcggcgtgt aggagtgccc ggggaaggtg aacacgccgc
      901 ggaccacgaa accggcgtcc gcggcatacc ggccgatctc gcccgccgcc tcgggcgaga
      961 cgccgctgcg gtggtgaccg gagtcaacct cgaccacgac ctccgggcgt atgccgtgcg
     1021 cggccatcgc gtccaggctc gccgtcgagt cgaccccgac cgccacgctg gcccactcca
     1081 gcaccgccgc caaccgtcgg gccttgggtg cgtcgaccca gagcgggtag gcgatgaaga
     1141 ggtcggcgca gccgccggcc gcgaagacct cggcctcccc caccgtggcg acggtcagcc
     1201 cgaccgcacc ggcctccagt tgcaggcgac ccagccgcac cgacttgtgg gtcttggcat
     1261 ggggtcgcag cccaacaccg agcgctcggg cccgagcagc catcgcctcg atcgacgcct
     1321 gggccttcgc cacgtctagg cggaggtagg gcgtgggcag ggaaggagcc gacatcccgg
     1381 cgtcagcctc gaagcccgtg cggcagcgcg tcgacgaact ccacggtctg cggcatgtcg
     1441 gcgtgaccgg ccagccggaa ccacttgacg gcccactgac ggcggacccg gcggacatcg
     1501 gtcaccgcgt cgttgtggaa gcggcgggcc aactggaccc gcaacccggt ggctcgcacc
     1561 cgcgacacga tgtcgtcgcc gaaacccccc gccccgggca gggacgccag tgtgtcggcg
     1621 gtcagggcca ggccgagggc ctcggacagg cgcgactcga ccgtctcgcg ctcgttgctg
     1681 aaccgctgcc ggtcggcgtc ggtgtcctcg tcctcggcag ccgacaacga gtcggacgcg
     1741 gcggaggcca gcatgagagc gctcgcggcg tcgaggactc gcgcgttggc caactccaag
     1801 gtggcttcgg ctcgccggac gagttgcgcg tccagagcgg ccagcgtgcc ctggacccgg
     1861 gcgtgcaagc ggtcgagccg ggcggcggtg taggtcaaat accacccgat cgctaccagg
     1921 ccgagggcca gcacgatcca caggttggga ccatccatca cggcagactc cttccgccgc
     1981 ggcgcaagcg accgaggacc ccatgaccag gcatggctcg agccgcggcc gccccctcgc
     2041 acaccgtttc gtagaccagc gtgatcgcgt cggccacgac tgaccagtcg aagaggtcgg
     2101 cacgggctcg gccggcagcc gccaggcggg cgcgttcggt gtcgtcgcgc agcagccgga
     2161 ccagttggcg ggccagatcg tccgggtcct cgttgacgaa cgtcgccccg accgttcccc
     2221 cgtcgagcac ccgcacgaag gcggggatgt cgctggcgag gacggcggcc ccggcgctca
     2281 tcgcctcgat gaggatgatc ccgaaactct ccccaccggt gttcggtgcg atgtagacgt
     2341 cgaccgagcg cagcagagcg gccttcgcgg cgtcgtcgac cgcgcccagg aactcggtcg
     2401 cggcggcgac ctcgggggtc atcgccgcgc gcacctcgtc cgcgtcgccg ggaccggcca
     2461 ccagcaaccg caacccgggc acctgcgcga gcaccgcagg gagcgccgca gcgagcacgg
     2521 gcatcccctt gcggggctcc cccatccggc cgaggaaggc gatcgtcggc cgacctggtt
     2581 gaccctgcca ctgcggccgg ggcagggtcg atgcgaatcg gtcgacgtac accccgttgg
     2641 ggatgaccac ggcgtcacca ccgaaatggg tcgtcagggt gcggcgggca tcctccgaca
     2701 cggcgatccg cccgaggatc ttctccatgc tcgagcgcag gaacggatag ctcacctgca
     2761 tggcgcgcga gcgcaggttc gaggtgtgga aggtcgccac gatcgggcag tcggcggccc
     2821 acagtgcgag cgcaccgatg ctcggcgtga tcggctcgtg gatgtggacc aggtcgaagt
     2881 gaccacggtc caaccacgca gtgaccttgg cgttggtcat cagcccgaag ttgagccgcg
     2941 ccaccgagcc gttgtagctc accggcacgg cgcgcccggc cgagacgacg tagtcgggta
     3001 gttcggtgcc ctcgtcggcg ggggcgagca ccgacacggt gtgaccgcgt gagcgcagga
     3061 tctcggccag gtcgcgcacg tggaactgca ccccgccggg gatgtcgaac gagtaggggc
     3121 agacgatgcc gatccgcacc cttggtcacc gccccagacg ggtcgggtcg aggtcgtcga
     3181 cgaaaacccg ctgcatcatg tgccagtcgg acgtgtgctc gcggatcgga ccttccacgt
     3241 atgccgcgca cgcctgggtc atcgcctggg ccttggccgc ggtgtcgccc tcggtgggca
     3301 ccgggatctc gtcggagaag gtcacgacgg tctcgcggcc tgcggcgcca cgccccggcg
     3361 ccgcgggcgc gaagtggatc gacgcggcat acagcggggc gccggtgagc agcgcgagga
     3421 gcgcggggcc cttggccatc cgggcgcggt ggccgaagaa gtcgacctcc acgccgttgc
     3481 tcgtcaggtc acggtcggag accagggcca cgaagtcgcc tcgctcgacg gcttcgcgta
     3541 gcccgttgaa cgggttgggc ccaccggtca ggggcaggat cgtcatgccg agcgactcgc
     3601 ggaactcgaa gaactcccgg aagacctcct cgggctccaa ccgctcggcg accgtcgtga
     3661 cgggagccag gtgcccggtc gaccacgccc cggcgatgtc ccagttgccc atgtgcggca
     3721 cgaagatcac ggcggagcgc cccgcgtcga ggttcgcacg cacggcagcg tcgttgaccg
     3781 cgcggcacat ccggtcgagg tcgtcgtggg tgcagtcagg cagccggaac gcctcacaga
     3841 aatagcgcgt gtaggaccgc atgccctcgc gcaccagggc atccagcgcg gcatcgtcga
     3901 gctcggggcg caccttggcg tagttcgcgc gcatccgcgc caacccctta ccgcctcgac
     3961 gcaccgatgc gtcggccacg gtgtcgaaga ctcggtatgc cgcccgctcc ggcatccgac
     4021 gcacggcact ccaaccggcg cggtagcccc agacggtgcc gcgggcccga gcggtcgcca
     4081 ggactcctga cagaccaccc atcaggtctc cgtcggcccg gagtcgccgg ccgtgcctgc
     4141 cccgtcggcc cggagggccg ccatggcttg ggtgtgcacc gtggccatcc gttggatcac
     4201 ggtgatgaaa ctggcgacgg acaggagggc gaggacgacg gtcagcaccc acaacgggag
     4261 gcccaatccg accaggccgg tggccagcag gacggcgaac aatcggtcgg cgcgctcggc
     4321 gatccccacg ttgcaggtca tgccgaggcc ttcggcccgg gccttggcgt aggacacgac
     4381 catgccaagg atgaggcaag ccagcgcgag cccggccatc agccagttgg cgcctggccc
     4441 ggcgaaccac agcacgaggc caccgaagat cgccgcgtcg ccgacccggt cgagggtcga
     4501 gtcgaggaat gctccccaga cgccttgccg accggacagg cgggccatca ccccgtcaac
     4561 gttgtcgctg aagatgaaga gcgtgatgag cagcgtgccg gtgaggaact ccccgcgggg
     4621 atagaacgcc agcgccgaga cacacacggc caaggtgccg atgatcgtca ccgcgtcggg
     4681 cgtcagcccg atggcgagga atgccttggc gatcggcgtc atgaccttgg tccaggacgc
     4741 gcgcagaagt cggttcagca tgagggcctc ggggtcggca tggtgcccac cagcctaggg
     4801 gtatgccgtg tggtcccggt gccgcggcgc accggtgggc gcgcggcata ccgtgcggct
     4861 caggccggcc agacctcggc gaggcgcgtc cgcacgtcct cgaggagcag cggcaacgcc
     4921 ttggtctggc cgatgatcgg caggaagttg gagtcgcctc cccaccgcgg cacgacgtgt
     4981 tggtgcaggt gggcggccac ccccgcgccg gcgacccggc cctggttcat gccgatgttg
     5041 aagcccatcg gagccgaggt ccgggtgagc gcggcgaggg cggtcttggt catcgccgtg
     5101 aactcggtgg tctcctcgtc ggtgaggtcg acgtagaggg ggacgtgccg ataggggcag
     5161 atgagcaggt ggcccgggtt gtaggggaag aggttcatga cgacgaagca caactcaccg
     5221 cgagcgacga tcagcccttc gatgtcgctg cgcagcgggg ctgcgcagaa cgggcacccg
     5281 tcgccggcct cctcggtggg ccggacgccc tcgatgtagg ccatccgatg cggcgtccac
     5341 aaccgctcga acccgtcctc gacgcgcgca aaatcgctcg cgctctcaag gtcggcagtc
     5401 gccctgccgg ggtcgtcgga cgtcacgacc cgagtctaga gggcggcttc ggcgaccggt
     5461 gaggggtctc tccgaaccta aacctgggtc cgggaggcga tcgcggcgag gatctcggcg
     5521 gcagcctcgg caacaggtat gccgttgcgc tggctcccgt cgcggaaccg gaaggacacc
     5581 gcgccggcgg cccggtcgtc ctcccccgcg atgagcacga acggcacctt cgacttggag
     5641 gcgttgcgga tcttcttggg gaagcggtcg tcgctctcgt ccaactcgac ccggacaccc
     5701 tgcgccgcga ggccgcctag gacctcccgg aggtaaccgg catactcctc ggcgacgggc
     5761 accccgagga cctggaccgg ggacagccag ggcgggaagg cgcctgcgta gtgctcgacc
     5821 aagaccccca tgaagcgctc gatcgacccg aacttcgcgg agtggatcat caccggttgc
     5881 tggcgggagc cgtcggcagc ctggtattcc aacccgaagc gctccggctg gttgaagtcg
     5941 tattggatcg tcgacatctg ccaggtgcgg ccaatggcgt cgcgggcctg gacggagatc
     6001 ttcggcccgt agaacgccgc gccgcccggg tcgggcacga ggtcgaggcc agactcggcc
     6061 gccacgtcag cgaggatctg cgtcgcctcg gcccactgct cttccgagcc gatgaacttg
     6121 cccttcttgg cgccggagtc gtcgcgcgtg gacagctcga ggtagaagtc gtccaggccg
     6181 aagtcacgca gcagcgacag cacgaagttc aacaggtgct tgatctcggc gggggcctgc
     6241 tcgggcgtga cgtaggagtg cgagtcgtcc atctccagac cgcgcacccg ggtgaggccg
     6301 tggatgaccc ccgacttctc ataccgatag accgacccga actcgaacag ccgcaacggc
     6361 aactcgcggt aggaacgtcc ccgcgagcgg tagatgaggt tgtgcatcgg gcagttcatc
     6421 gctttgaggt agtagtccga cccctccatg ctcatgggag ggaacatcgt gtcggcgtag
     6481 tagggcaggt gcccggaggt gtggaagagc ccctccttgg agatgtgcgg cgtgccgacg
     6541 tagaggaagc cctcctcgat gtgccgccga cgcgcgtagt cctccatttc ccgcttgagc
     6601 accccgccct tggggtggaa caccggaagc ccgctgccga gctcgtccgg gaaggagtag
     6661 aggtcaagct cgatcccgag tttgcggtga tcgcgccgct ccgcctcggc cagccggtcg
     6721 aggtatgccg tgaggtc
//
LOCUS       DAORPW010000124         6415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3203_length_6415_cov_1027.079403, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000124 DAORPW010000000
VERSION     DAORPW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3203_length_6415_cov_1027.079403"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /gene="ftsH"
                     /locus_tag="PLB86_09645"
     CDS             complement(<1..1375)
                     /gene="ftsH"
                     /locus_tag="PLB86_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRC65438.1"
                     /translation="MTTKRAIRPQWLIFILLAVGLTAFLMFFSDSGGYTRVDTSAALD
                     LIATDKVDTARVVNNERIELALKDGVTYSDGARVKDATKVYAFYVDARGPQIVKALDD
                     HPPAKGFTDQIDTQNWFASMLSLVLPMIIVLGLFWFLMGQMQGGGNRVMQFGKSRAKL
                     ASKDMPKVTFADVAGADEAVEELHEIKEFLQDPARFLAVGAKIPKGVLLYGAPGTGKT
                     LLARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFEQAKANAPAIVFVDEIDAVG
                     RHRGAGLGGGHDEREQTLNQLLVEMDGFDVKTNVILIAATNRPDILDPALLRPGRFDR
                     QIAVEAPDMIGRHRILQVHSQGKPMAPDVDLLAVARRTPGFTGADLANVLNEAALLTA
                     RTNKRAIDNQALDEAIDRVIAGPQKRTRIMSAKERKITAYHEGGHAIVAAAMNHTDPV
                     TKVTILPR"
     gene            complement(1528..2079)
                     /gene="hpt"
                     /locus_tag="PLB86_09650"
     CDS             complement(1528..2079)
                     /gene="hpt"
                     /locus_tag="PLB86_09650"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRC65439.1"
                     /translation="MDAPSMGSGLTRVLLTEEQILTRLDELATQIWADYAGKDLLLVG
                     VLKGAVLVMADLMRAMPGSVRMDWMAISSYGSGTKSSGVVRIIKDLDTDITNKHVLVV
                     EDIIDSGLTLSWIMANLSSRKPASLEICTLLRKPEVAKVKIDVKYVGFDIPNEFVVGY
                     GLDFAEDYRNLRCIGTLAPSVYS"
     gene            complement(2140..3210)
                     /gene="tilS"
                     /locus_tag="PLB86_09655"
     CDS             complement(2140..3210)
                     /gene="tilS"
                     /locus_tag="PLB86_09655"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRC65440.1"
                     /translation="MPGPPAAVAAVRLAVRGVLADLPTWSTVLVACSGGADSLALAAA
                     LAFEAPGAGLRAGAVIVDHGLQEGSADVAAQAAQACRGLGLDPVEVVRVAVPDSPTGP
                     EDAARTARYAALADAATRHTAALVLLGHTRDDQAEQVLLGLARGSGARSLSGMPPSRG
                     PYARPLLGVTRAQTEAACREAGLEPWRDPHNSDPTYARVRARAALGELDAVLGPGLGA
                     ALARSADLLRDDADLLDELAAQARADVDLDPAGERVVAAQAGAGDVPAVRVAVAGLAA
                     LPPALRRRVWRIVAAEAGARALSAAHVALLDGLVLRWHGQGAIDLPGGVVALRVGGTI
                     QMGPRPADVVARPVAQTSQPVR"
     gene            complement(3227..4318)
                     /locus_tag="PLB86_09660"
     CDS             complement(3227..4318)
                     /locus_tag="PLB86_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRC65441.1"
                     /translation="MSDQPVDRPSDHPGDAPGPFIDWDFARVTAARLAAPGPKVNAGE
                     AAGVVAGLRQAALAAHDPVAETSRLTSPPGSAPVLIVDRAGWASANIDSMRELIDPVV
                     AKMAAGMPSQSGEGMAAMGGKITGAETGALLAFFSSKILGQYDLAPSGQPRLLLVAPN
                     IVSAEREMGVSPRDFRLWVAMHEETHRVQFTAVPWLREHMIERSRTLALELAPTPSEF
                     AERVQQIAQGLPGAFRPGSTGLAEVFLTPEQKGKIAEITAVMSLLEGHADVIMDDVGP
                     SVIPTVAEIREKFTQRRAGLGQLDRLIRRLLGLEAKMRQYADGAVFVRAVVDRVGIDG
                     FNAVWTSPQTLPTAAEMADPQAWVARVHG"
     gene            complement(4338..5753)
                     /gene="dacB"
                     /locus_tag="PLB86_09665"
     CDS             complement(4338..5753)
                     /gene="dacB"
                     /locus_tag="PLB86_09665"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRC65442.1"
                     /translation="MRRVTAVALAGLLAIGGYGTADVLDLAPGILTLAPEAAAYPTAP
                     SPTTSAGATAHLPLPSAAPSGMPVAPVDTTAPLPSVAGIKAAVAPALADPDLKGAAVV
                     VRDALTGADLLDVSGSEPRIPASTLKLIAALAVQQVFPDGTTLVTRVVQGASPDTVVL
                     VAGGDMFLAPDKGDPAAVVGRAGLGDLADSVATALRAKAITQVTVQVDTSYAPGPQVA
                     VGWQPAFRTLGIVGPVAMLGLSTQWAQSGKPGPADPVAEVVAAFAARLTERGITAVAG
                     GPASGAGVSLGEVRSAPVTDVLGLALERSDNTVTESLARQAAFRVGRPSDFEGTAAYL
                     REALTLAGIDPRGMSVHDASGLSRTSHVPARVLADVLDLGVKGTVPGFTQALRHLPVA
                     GLTGTLGTRFTAAPEAPAAGLVRAKTGSLNGVNSLAGTVVTADGRMLVFAIMQEGPVG
                     TTDARAALDRFGAALATCGCR"
     gene            5834..6355
                     /locus_tag="PLB86_09670"
     CDS             5834..6355
                     /locus_tag="PLB86_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRC65443.1"
                     /translation="MEFDVTIEIPKGHRNKYEVDHATGRIRLDRMLFTSMAYPGDYGY
                     IEDTLGEDGDPLDAVILLDEPTFPGCVVRARAIGVFQMTDEAGGDDKILCIPAGDPRQ
                     AHITELDQINKFETAAIGHFFDTYKDLEPEKSVDASRWFGRVVAERVIIEAIERARAA
                     GHTTARWRPTESH"
BASE COUNT          982 a         2212 c         2331 g          890 t
ORIGIN      
        1 cgcgcggcag gatcgtcacc ttggtcacgg ggtcggtgtg gttcatcgcc gcggccacga
       61 tggcgtgacc accctcgtgg tatgccgtga tcttgcgttc cttggcggac atgatccggg
      121 tgcgcttctg cgggccggcg atgacccggt cgatggcttc gtccagggcc tggttgtcga
      181 tcgcccgctt gttggtgcgg gcggtgagca gcgcggcctc gttgaggacg ttggccaggt
      241 cggcgccggt gaagccgggg gtgcgtcggg cgacggccaa caggtcgacg tcgggagcca
      301 tcggcttgcc ctgcgagtgg acctgcagga tccggtggcg accgatcatg tcgggggcct
      361 cgacggcgat ctggcggtcg aagcggcctg gacgcagcaa cgccgggtcg aggatgtcgg
      421 ggcggttggt cgccgcgatg aggatgacgt tggtcttgac gtcgaagccg tccatctcga
      481 ccaggagctg gttgagggtc tgctcacgct cgtcgtgccc accaccgagg ccggcaccac
      541 ggtggcgacc gacggcgtcg atctcgtcga cgaagacgat ggccggggcg ttcgccttgg
      601 cctgctcgaa gaggtcccgc acgcgggagg cgccgacacc gacgaacatc tcgacgaagt
      661 ccgagccgga gatcgaatag aacgggacgc cggcctcgcc ggccacggcc cgggccagga
      721 gggtcttgcc ggtgccaggc gcgccgtaaa gcaggacgcc cttggggatc ttggcgccga
      781 ccgcgaggaa gcgggccggg tcttggagga actccttgat ctcgtggagc tcctcgacgg
      841 cctcgtcggc accggccacg tcggcgaagg tcaccttggg catgtccttg gaggccagct
      901 tggcgcgcga cttgccgaac tgcatcaccc ggttgccgcc accctgcatc tggcccatga
      961 ggaaccagaa caggccgagg acgatgatca tcggcaggac caggctgagc atcgacgcga
     1021 accagttctg ggtgtcgatc tggtcggtga agcccttggc cggcgggtgg tcatcgagcg
     1081 ccttgacgat ctgcggaccg cgggcgtcga cgtagaaggc gtagaccttc gtcgcgtcct
     1141 tgacccgggc gccgtcggag taggtgacgc cgtccttgag cgcgagctcg atccgttcgt
     1201 tgttgacgac gcgggcggtg tcgaccttgt ccgtggcgat gagatccagg gcggcggacg
     1261 tgtcgacccg cgtataaccc cccgagtctg agaagaacat caggaaggcc gtcaaaccga
     1321 cggcaagcag gatgaagatc agccactggg ggcgaatcgc gcgtttcgta gtcatgcgtg
     1381 tgcggggcgc tgccccagcc tcctggtcgt cgtgcgtcgc tcaccggcgc tgcggccacc
     1441 acagggacgc tcgccgaaag ggattctcgc aggtcaacgc ccggctgtgc gcccgtgttc
     1501 cgccgtcacg cgatcactgt cacgcgatca gctgtagacg gaaggagcca gggtgccgat
     1561 gcagcgcagg ttgcggtaat cctcggcgaa gtccagcccg tagccgacga cgaactcgtt
     1621 ggggatgtcg aagccgacgt acttgacgtc gatcttcacc ttggcaacct cgggcttgcg
     1681 cagcagcgtg cagatctcca gcgaggccgg cttgcgggag ctgaggttgg ccatgatcca
     1741 cgagagggtc aggccggagt cgatgatgtc ctcgacgacg aggacatgct tgttggtgat
     1801 gtcggtgtcg aggtccttga tgatgcgcac gacacccgag gacttcgtgc ccgagccgta
     1861 gctcgagatg gccatccagt ccatccggac cgatccgggc atcgcccgca tgaggtcagc
     1921 catgacgagc acggccccct tgagcacgcc aacgagcaac aggtccttgc ccgcgtagtc
     1981 ggcccagatc tgcgtggcca actcgtcgag ccgggtgagg atctgctcct cggtgaggag
     2041 gaccctcgtc agtcccgacc ccatgctcgg tgcgtccacg acgtagtcct cctggcgtta
     2101 gccggcctcg tgggccgtcc ggtcgcggtc gagtcgaccc tatcgaaccg gttgcgatgt
     2161 ctgggcaacg ggacgagcca ccacgtctgc ggggcggggt cccatctgaa tggtcccacc
     2221 cacccgcagc gcgaccacgc cacccggcag gtcaatcgcc ccttgcccgt gccaccgcag
     2281 gaccagtccg tcgagcaggg cgacgtgggc ggccgacagc gcgcgcgccc cggcctccgc
     2341 ggcgacgatc cgccagaccc tccggcgcaa ggcggggggc agggcggcca ggcccgccac
     2401 cgctacccga acggccggca cgtccccggc gcccgcctgg gcagccacga cccgttcccc
     2461 cgccgggtcg aggtccacgt cggcgcgcgc ctgagcggcc aactcgtcca acaggtcggc
     2521 gtcatcacgc agcaggtcgg ccgagcgggc cagcgccgcc ccgagcccgg ggcctagcac
     2581 cgcgtccaac tcccccaggg ccgcccgggc gcgcacccgc gcataggtcg ggtcggagtt
     2641 gtgcggatcc cgccacggct ccaggcccgc ctcgcggcag gcggcctcgg tctgcgctcg
     2701 agtcaccccc aagagggggc gggcataggg accgcgggag ggcggcatac cggagaggga
     2761 tctggccccg gacccgcggg cgaggccgag cagcacctgc tccgcctggt cgtcgcgggt
     2821 gtggccgagc aacaccaagg ccgcggtatg ccgcgtggcc gcgtccgcga gcgcggcata
     2881 ccgggccgtc cgggcggcgt cctcgggacc ggtcggtgag tcgggcacgg cgacccgcac
     2941 gacctcgacc ggatccagac ccaacccgcg acaggcctgc gccgcctgcg ccgcgacgtc
     3001 ggcggagccc tcctgcaacc cgtggtcgac gatcacggca ccggcgcgca ggccggcccc
     3061 aggggcttcg aaggcgagcg ctgccgcgag cgcgagcgaa tcggcacctc ccgagcaggc
     3121 caccagcacc gtcgaccagg tcggcaggtc ggccaagacc ccccgcaccg cgaggcggac
     3181 cgccgccacc gctgccgggg gaccaggcac aggtatgccg cccggctcag ccgtggacgc
     3241 gcgcgaccca cgcctgcggg tcggccatct cggcggcggt cgggagcgtc tgcggggagg
     3301 tccagacggc gttgaagcca tcgataccca cgcggtcgac gacggcgcgg acgaacacgg
     3361 cgccatcggc atactggcgc atcttggcct ccaacccgag cagccggcgg atcagccggt
     3421 cgagttgccc gaggccggcg cggcgttggg tgaacttctc gcggatctcg gcgaccgtgg
     3481 ggatgaccga cgggccgacg tcgtccatga tcacgtcggc gtgcccctcg agcagcgaca
     3541 tcacggcggt gatctcggcg atcttgccct tctgctcagg cgtgaggaac acctcggcca
     3601 gcccggtgct gcctggccgg aaggctcccg gcagaccctg ggcaatctgc tggacccgct
     3661 cggcgaactc gctcggcgtc ggcgccaact cgagggccag ggtgcgggag cgctcgatca
     3721 tgtgctcgcg cagccacggg acggcggtga actggacccg gtgggtctcc tcgtgcatcg
     3781 ccacccacag gcggaaatcg cggggtgaga cgcccatctc acgctcggcc gacacgatgt
     3841 tgggcgcgac gaggagcagc cgcggctggc ccgagggggc gagatcgtat tggccgagga
     3901 tcttgctgga gaagaaggcc agcagcgcac cggtctcggc tccggtgatc ttgccgccca
     3961 tggcggccat cccctcaccg gactgggacg gcataccggc ggccatcttg gccacgacgg
     4021 ggtcgatgag ctcgcgcatc gagtcgatgt tggccgacgc ccacccggcg cggtcgacga
     4081 tgaggaccgg cgccgacccg gggggcgagg tcagtcggga cgtctcagcg accgggtcat
     4141 gggccgccag ggccgcctgg cgcaagccgg caaccactcc tgcggcctcg ccggcgttga
     4201 ccttggggcc gggggcagcc aggcgcgccg cggtgactcg cgcgaagtcc cagtcgatga
     4261 atggccccgg cgcgtcaccg gggtggtcgg acggtcggtc gacaggctgg tcgctcaccg
     4321 cgggaatccc ttcatggtca acggcacccg caggtcgcga gcgccgctcc gaaccggtcc
     4381 aacgcggccc gtgcatcggt cgttcccacc ggcccctcct gcatgatcgc aaagacgagc
     4441 atccggccgt ctgcggtgac gaccgttccg gcgagcgagt tcaccccgtt gagcgagccg
     4501 gtcttggccc gaacgaggcc ggcggccggg gcctcggggg ccgcggtgaa gcgcgtcccc
     4561 aaggtgcccg tcagccccgc caccggcaag tggcgcagcg cctgggtgaa gcccgggacg
     4621 gtgcccttca caccgaggtc gaggacgtcg gccaggaccc tggccgggac gtgactcgtg
     4681 cggctgagcc ccgacgcgtc gtgcacgctc atcccgcgcg gatcgatccc cgcgagggtg
     4741 agggcctcgc gcaggtatgc cgccgtcccc tcgaagtcgc tcggccgccc cacccggaag
     4801 gcagcctgac gggcgagcga ctcggtcacg gtgttgtcgc tccgctccag ggccaggcca
     4861 aggacatcgg tgacgggcgc ggagcggacc tcgccgagcg acaccccggc cccggacgcg
     4921 ggccccccgg ccacggcggt gatgcctcgt tcggtgagcc gcgcagcgaa ggccgcgacc
     4981 acctcggcaa cgggatcggc cgggcccggc ttgccgctct gggcccattg ggtcgacagg
     5041 ccgagcatcg ccaccgggcc gacgatcccg agggtgcgga acgccggttg ccagccgacc
     5101 gccacctgcg gacccggcgc gtaggaggtg tccacctgaa ccgtcacctg ggtgatcgcc
     5161 ttggcgcgca acgcggtggc caccgagtcg gccaggtcac ccagcccggc ccgacccacg
     5221 accgcggccg gatcgccctt gtcgggtgcc aggaacatgt ctcccccggc gacgagcacc
     5281 acggtgtcgg ggctggcgcc ctggacgacg cgggtcacga gtgtcgtccc gtcggggaag
     5341 acctgctgga ccgctagggc ggcaatgagt ttgagcgtcg acgccgggat ccggggctcg
     5401 ctgccggaca cgtcgagcag gtcggcgccg gtgagcgcgt cgcgcacgac gacggcggct
     5461 cccttgaggt ccgggtcggc caaggcgggt gcgacggcgg ccttgatccc cgccacgctg
     5521 ggcagcggcg ccgtcgtgtc gacgggggcg accggcatac cggatggggc ggccgagggc
     5581 agcgggaggt gagccgtggc acccgccgag gtggtcggcg agggggcggt cgggtatgcc
     5641 gcggcctccg gcgcgagggt gaggatgcct ggcgcgaggt ccaggacatc ggccgtgccg
     5701 tagcccccga tggcgaggag gccggccaag gcgaccgccg tgacgcgacg caaggtctca
     5761 ccccctagtc tgtgaactga ccaacgacaa ccgaagcgta ggtcacccga gaccagcgaa
     5821 gaggggcacg gacgtggagt tcgacgtcac catcgagatc cccaagggac accgcaacaa
     5881 gtatgaggtc gaccatgcga cgggccggat ccggctcgac cgaatgctgt tcacctcgat
     5941 ggcctacccg ggtgactacg gctacatcga ggacaccctc ggcgaagacg gcgacccgct
     6001 cgacgcggtg atcctgctcg acgagccgac cttccccggg tgtgtcgtgc gggcccgcgc
     6061 gatcggcgtc ttccagatga ccgacgaggc cggcggcgac gacaagatcc tgtgcatccc
     6121 ggccggtgac ccgcgccagg cgcacatcac cgagctcgac cagatcaaca agttcgagac
     6181 cgcggcgatc gggcacttct tcgacacgta caaggacctc gagccggaga agtcggtcga
     6241 cgcctcgcgc tggttcggtc gcgtcgtggc ggagcgggtc atcatcgagg cgattgagcg
     6301 ggctcgggct gccggccaca cgactgctcg ctggcgtccc accgagagcc actgaggcct
     6361 cgctacggga gggcccggtt agggacggcc gccgtagggg atcaagtcgg ggcgc
//
LOCUS       DAORPW010000125         6395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3225_length_6395_cov_694.482334, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000125 DAORPW010000000
VERSION     DAORPW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3225_length_6395_cov_694.482334"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1179
                     /locus_tag="PLB86_09675"
     CDS             67..1179
                     /locus_tag="PLB86_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HRC65444.1"
                     /translation="MGLFDKVEKGLERTVNRAFAKAFRSEVQPVEIAGAIRNAMDDRA
                     SVLGPGRTMVPNHYTIELSTTDFGRLDEYSDSITEDLIAAAAEHAETQRYLPGGPIEI
                     VLAEGSDLDTGVFRVRPETSKRGQASGMPPAPVPAPPHRPAAPVHKPAPVRAPAPAAP
                     AAPPAAPAAQAPALAPQPAPDALAAPDLDAGHLNAGHVEPPEVIGDWANELSHPAYDA
                     SSHAADPDVELLPDRALAPDQPAVAPPPPPPLAAPPAAAKPAPAPPAKLPRINPAQRP
                     WLDIDGERYPLLGSLTVIGRDATADVILDDPGISRRHSEVRVTHDGPHLVSSIRDLAS
                     TNGTFVNGERIASTRLIDGDQITVGRTAITFRAGKR"
     gene            1184..1756
                     /locus_tag="PLB86_09680"
     CDS             1184..1756
                     /locus_tag="PLB86_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRC65445.1"
                     /translation="MSELTLTLMRLGLLVMLWLFVFAVVGVLRSDLFGTRVSRRTRRR
                     AAQTGVPVGAAPASGYAAPAPSPHGAPPARQAARPAPARQSGRGGAGARGIPTSVAVT
                     EGRLAGTVIPLTPAGILVGRNPECSLVLDDDFTSGRHLRLFLREGSWWVEDLGSTNGT
                     FLDAARLTTAQPVGVGSVLRIGRTALELHG"
     gene            1756..3150
                     /locus_tag="PLB86_09685"
     CDS             1756..3150
                     /locus_tag="PLB86_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRC65446.1"
                     /translation="MTTALRYAARTHLGLGPKSRNEDSGYAGPHLLVLADGMGGHAAG
                     DVASSMIVGALAPLDEEIVSGEQALRVLESELREANAQLRAAMREDSGLAGMGATTIA
                     MLRSGNKLAMAHIGDSRAYLLRDGTFSQITKDHSFVQQLVDEKRITEAEAQHHPQRSL
                     VTRVMTGNPDDQPDLSIRELIPGDRYVLCSDGLTDFVSPDIVAEIITAAPTPEAAAER
                     CIEVALKASTRDNVTVVVADVVDSNELADLPSTPEIVGAAAVRGRARRKTRAIPVSPA
                     EKAAALTREVSGTPDDEPLQLAEEVASPRVLWLRRTAVGLAAAVLVVGAGYAGYDWTQ
                     RQYFLGAQDGYVTLYRGVNQTLGPIGLATFVRGTDILVTDLPARYQELITASISLGGL
                     AEADGRIASLREVAAECRAQNAAGIACGTTLPVATPSVTLPTSTSTATSTSTATSTST
                     GTGTVGFPVTPGAT"
     gene            3147..>4462
                     /locus_tag="PLB86_09690"
     CDS             3147..>4462
                     /locus_tag="PLB86_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRC65447.1"
                     /translation="MTTTVETIPTKSRRNTELLLLLFAVAIVLFAWVDTEVANRGAVP
                     AGLVAIGGGLLVLVLLFHLVLRWKASYADPLLLPIATLLNGLGLVMIHRLDLAAGDYG
                     FDGDAFRQLIWTGVSIIVAGALLWTLRDHRTLRRYTYTAMALGVLLLVLPLVPGLGSE
                     QLGSRIWIRVGPMSFQPGEIAKIALAVFFAGYLVQARDVLSLAGRKVLGFTFPRGRDL
                     GPIMVAWLVSVAVLVFEKDLGSSLLFFGLFVAMLYVATERVSWIAIGLMLFAAGAYAA
                     YLLFPHLQRRVMLWLDPFSSDSLAQSDQLAKGLMGMAAGGLFGTGLGKGRPWLVAFAE
                     SDFIVPSLAEELGLTGLFAILLLYGLFVQRGLRTALGVRDGFGKLLALGLAFSVALQV
                     FVVLGGVTRVIPLTGLTTPFMSLGGSALLANWSITALLLRISDQARR"
     assembly_gap    4463..4562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4598..6055
                     /locus_tag="PLB86_09695"
     CDS             4598..6055
                     /locus_tag="PLB86_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRC65448.1"
                     /translation="MNTPILRMSRLVGALFAILLISATIIQFVQAPSLRARPDNRRTL
                     LDNYSRDRGSILVGDTAIATSTPSEGELAYLRTYPQGPLYSHVTGYYSYVYGAGGGLE
                     GSSDGLLSGSSDALFYKRIADLLTGKQPTGASLELTLNAKAQEAASKALGNQRGAVVA
                     LDPRTGAILALVSHPEYDPNTLANHDSNAQKTAWDGMLDDRTRPLVDRTISGNLYPPG
                     SVFKIVTTAAALSTGAVNETTQIPGPAVMDLPQTTVGLPNHDGKSCGPGDLTTLQHAL
                     EISCNTAFGWIGLKVGGDALRDQAAKFGFGESLRIPMRVTPSVFPSSLNPPQTAQSAI
                     GQFDVRVTPLEVAMVSAAIANRGVVMRPYLVQQVVGANLEILDTHTPEQMSVAVTPEI
                     AAIISRMMVGVVDQGSGKPARINGVSVAGKTGTAEQGNGLPAHAWFTSFAPADNPQIA
                     VAVIVEDGGNAGDEAGGGRTAGPIAKAVMEAVITR"
     gene            6061..>6395
                     /locus_tag="PLB86_09700"
     CDS             6061..>6395
                     /locus_tag="PLB86_09700"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002858197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRC65449.1"
                     /translation="MTPGLGDVLGGRYTLTDRIAAGGMGDVWAATDRVLGRTVAIKIM
                     RPNTTDEGNFAARFRDEARHTAALSHPNIAAVYDYGEDDGTAYLVMELVPGLPLSSMI
                     AEGPMPPEQV"
BASE COUNT          916 a         2498 c         1964 g          917 t
ORIGIN      
        1 acaggcgccg ccggatacca tgtcaagacc gccgaaccac ggccgacgac acggaaggag
       61 gcgggcatgg gcttgttcga caaggtcgag aagggcctgg agcgcaccgt caaccgcgcc
      121 ttcgccaagg ccttccgctc cgaggtgcag ccggtggaga tcgccggggc catccgcaac
      181 gccatggacg accgcgccag cgtcctcggg ccgggccgca cgatggtccc caaccactac
      241 accattgagc tctcgacgac cgatttcggc cgcctcgacg aatactccga ctccatcacc
      301 gaagacctca tcgccgccgc cgccgagcac gccgagaccc agcgctacct ccccggcggc
      361 ccgatcgaga tcgtcctcgc cgagggctcc gacctcgaca caggcgtgtt ccgcgtccgt
      421 cccgagacgt ccaagcgcgg ccaggcatcc ggtatgccgc ccgcccccgt cccagccccg
      481 ccgcaccgcc cagccgcccc ggtccacaaa ccggccccgg tccgtgcgcc agcaccggca
      541 gccccggcgg cgcccccggc cgcacccgca gcccaagccc cagccctcgc acctcaaccg
      601 gcgccggacg ccctcgccgc ccccgacctc gacgccggcc acctcaacgc cggccacgtc
      661 gagccgcccg aggtcatcgg tgactgggcc aacgagctga gccacccggc atacgacgcc
      721 tccagccacg cggccgaccc ggatgtcgag ttgctgcccg accgggccct cgcacccgac
      781 cagcccgcgg tcgcgccccc gccgccaccc ccgctcgcgg ccccgcccgc agccgccaaa
      841 cccgcaccag cgccccccgc caagctgccc cggatcaacc cggcccagcg cccctggctc
      901 gacatcgacg gcgagcgcta ccccctgctg ggctccctca ccgtcatcgg ccgcgacgcc
      961 accgccgacg tcatcctcga cgacccggga atctcgcggc gacacagcga agttcgcgtc
     1021 acgcacgacg gcccgcacct cgtgtcgagc attcgcgacc tggcctcgac caacggcacc
     1081 ttcgtcaacg gcgagcggat cgccagcacc cggctcatcg acggcgacca gatcaccgtc
     1141 gggcggaccg ccatcacgtt ccgcgccggg aagaggtgag ccggtgagcg aactcaccct
     1201 caccctcatg cggttggggt tgctcgtcat gttgtggctg ttcgtcttcg cggtcgtcgg
     1261 ggtgctgcgt agcgacctgt tcggcacccg agtctcccgc cgcacccgac gccgggccgc
     1321 ccagaccggc gtccccgtcg gcgcagcacc cgcctccggg tatgccgccc ccgcaccctc
     1381 gccgcacggc gcaccccctg ctcgtcaggc cgcccgtccg gcgccggccc gccagagcgg
     1441 tcgaggcggc gcgggagcac gcggcatacc gacgagcgtg gccgtcaccg aaggtcggct
     1501 cgcggggacg gtcatcccgc tcaccccggc ggggatcctc gtcggacgca accccgaatg
     1561 ctcgctggtc ctcgacgacg acttcacctc tgggcgacac ctgcggctgt tcctgcgcga
     1621 agggtcctgg tgggtcgagg acctcggctc gaccaacggc accttcctcg acgccgcccg
     1681 actgaccacg gcccagccgg tgggcgtcgg cagtgtgctg cggatcgggc gcaccgcgct
     1741 ggagctgcac ggctgatgac gacggccctg cgctatgccg cgcgcaccca cctcggcctc
     1801 ggccccaaga gtcgcaacga agactccggg tatgccggtc cccacctgct cgtcctcgcc
     1861 gacggcatgg gtgggcacgc tgccggcgac gtcgccagct cgatgatcgt cggggccctc
     1921 gccccgttgg acgaggagat cgtctcgggc gagcaggcgt tgcgggtgct cgagagcgag
     1981 ttgcgcgagg ccaatgccca actgcgcgcg gcgatgcgcg aggacagcgg cctggccggc
     2041 atgggcgcca cgacgattgc catgctgcgc tcgggcaaca agctggccat ggcccacatc
     2101 ggcgactccc gggcctacct gctgcgcgac ggcaccttca gccagatcac caaggaccac
     2161 tcgttcgtcc agcaactcgt cgacgagaag cgcatcaccg aggctgaggc gcaacaccac
     2221 ccgcagcgct ccctcgtcac ccgggtgatg accggcaacc ccgacgacca gcccgacctg
     2281 tcgatccgcg agctcatccc cggcgaccgt tacgtcctgt gctccgacgg gctcaccgac
     2341 ttcgtcagcc ccgacatcgt cgccgagatc atcaccgcgg cgcccacccc cgaggcagcc
     2401 gccgagcgct gcatcgaggt cgctctcaag gccagcaccc gcgacaacgt caccgtggtg
     2461 gtggccgacg tcgtcgacag caacgagctg gccgacctgc ccagcacgcc tgagatcgtc
     2521 ggtgccgccg ccgtgcgcgg ccgcgcccga cgcaagaccc gcgccatccc ggtgagcccc
     2581 gccgagaagg ccgccgcact cacccgcgag gtgtccggca cccccgacga cgagccgctg
     2641 caactcgccg aggaggtcgc ttcgccccgc gtgctgtggt tgcgccgcac cgcggtcggg
     2701 ctcgctgccg ctgtgctcgt cgtcggcgcg gggtatgccg gttacgactg gacccagcgg
     2761 cagtatttcc tcggcgccca ggacggctac gtcaccctct accgcggggt caaccagacc
     2821 ctcgggccga tcggcctcgc gaccttcgtg cggggcaccg acatcctcgt caccgacctg
     2881 cccgcgcgct atcaagagct gatcaccgcc tcgatctcgc tcggcgggct cgccgaggcc
     2941 gacggccgga tcgcgtcgct gcgtgaggtc gctgcggagt gccgggccca gaatgccgcc
     3001 ggaatcgcct gcggcacaac gcttccggtg gccacgccga gcgtgaccct gccgacgtcc
     3061 acctccaccg ccacgtccac ctcgaccgcc acgtccacgt cgaccggcac cggcaccgtc
     3121 ggctttccgg tgacgcccgg agcgacatga cgaccacggt cgagaccatc ccgacgaaat
     3181 cgcggcgcaa caccgagttg ctgctgctgc tcttcgcggt cgcgatcgtc ctcttcgcct
     3241 gggtcgacac ggaggtcgcc aaccgcgggg ccgtccctgc cgggctagtg gccatcggcg
     3301 gcggcctgct ggtcctcgtc ctgctcttcc acctggtgct gcggtggaag gcgtcgtatg
     3361 ccgacccgct gctgctgccc atcgcgaccc tgctcaacgg gctcggcctg gtgatgatcc
     3421 accgcctcga cctcgcggca ggtgactacg gcttcgacgg cgacgcgttc cggcaactca
     3481 tctggaccgg cgtctcgatc atcgtggcgg gcgcgctgct ctggaccctg cgcgaccacc
     3541 ggaccctgcg ccgctacacc tacaccgcga tggccctcgg tgtcctgctg ctcgtcctgc
     3601 ccctcgtgcc ggggttgggc tcggaacaac tcggctcacg gatctggatc cgggtcggtc
     3661 cgatgtcctt ccagcctggg gagattgcga agatcgctct cgcggtgttc ttcgctggct
     3721 acctcgtgca ggcccgcgac gtgctctcct tggccgggcg taaggtcctt ggcttcacct
     3781 tcccgcgcgg gcgtgacctg ggcccgatca tggtcgcctg gctggtgagc gtcgccgtcc
     3841 tcgtcttcga gaaggacctg ggctcgtcgc tgctcttctt cggcctcttc gtcgcgatgc
     3901 tctacgtcgc caccgagcgg gtctcctgga tcgccatcgg cctcatgctg ttcgctgccg
     3961 gcgcctacgc ggcatacctg ctcttcccgc acctgcagcg gcgcgtgatg ctctggctgg
     4021 acccgttctc gtccgactcc ctcgcccagt ccgaccaact ggccaagggc ctcatgggga
     4081 tggccgcggg cgggctgttc ggcaccggtc tgggcaaggg acggccgtgg ctggtcgcct
     4141 tcgccgagag cgacttcatc gtcccctcgc tggccgagga gctcgggctg accgggctgt
     4201 tcgcgatctt gctgctctac ggcctgttcg tccagcgcgg tctgcgcacg gccctggggg
     4261 tgcgtgacgg cttcggcaag ttgctcgccc tggggctggc gttctcggtc gcactgcagg
     4321 tcttcgtcgt cctcggtggc gtcacgcggg tcatcccgct caccggactg acgacgccgt
     4381 tcatgtcgct cggcgggtcg gccctgctgg ccaactggtc gatcaccgcg ttgctgctgc
     4441 ggatctccga ccaggcccga cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nngccgtcgc tgcgccgacc gaggcggtgc cacgaccgtg aacaccccca tcctgcggat
     4621 gtcccgcctc gtcggcgccc tcttcgccat cctgctcatc tcggcgacga tcatccagtt
     4681 cgtccaagcc ccctcgttgc gagcgcggcc ggacaatcgg cgcaccctgc tggacaacta
     4741 ctcccgcgac cgtgggtcga tcctcgtcgg ggacaccgcg atcgcgacct cgacgccgtc
     4801 cgagggcgag ctggcctacc tgcgcaccta cccccaggga ccgctctact cccacgtcac
     4861 cggctactac tcctacgtct acggcgcggg cggtggcttg gagggcagct ccgacgggtt
     4921 gctgtccgga tcatcagacg cgctgttcta caagcggatc gccgacctgc tcaccggcaa
     4981 gcaacccacc ggcgccagcc tggagctgac cctcaacgcc aaggcccagg aggccgccag
     5041 caaggccctg ggcaaccagc gcggtgcggt cgtcgccctc gacccccgca ccggcgccat
     5101 cctcgccctc gtcagccacc ccgaatacga ccccaacacg ctggccaacc acgactccaa
     5161 cgcccagaag actgcctggg acggcatgct cgacgaccgg acccgccccc tggtcgaccg
     5221 aaccatcagc ggcaacctct acccgcccgg ctcagtgttc aagatcgtca ccaccgcagc
     5281 cgcgctgtcc acgggggccg tcaacgagac gacgcagatc cccggcccgg cggtcatgga
     5341 tctgccgcag accaccgtcg gcctgcccaa ccacgacggc aagtcgtgcg gccctggcga
     5401 cctcaccacg ttgcagcacg ccctcgagat ctcctgtaac accgccttcg gatggatcgg
     5461 cctcaaggtc ggcggcgacg ccctgcggga ccaggccgcc aagttcggct tcggtgagag
     5521 cctgcggatc cccatgcggg tcactcccag cgtcttcccc agctccctca acccgcccca
     5581 gaccgctcag tcggccatcg ggcagttcga cgtgcgagtc acccccctgg aggtcgccat
     5641 ggtgagcgca gccatcgcca accgcggcgt cgtcatgcgc ccctacctcg tccagcaggt
     5701 ggtcggcgcc aacctcgaga tcctcgacac tcacaccccc gagcagatgt cggtggccgt
     5761 cacgcccgag atcgccgcca tcatctcgcg gatgatggtg ggcgtcgtcg accagggctc
     5821 cggcaagccg gcccgcatca acggcgtctc ggtcgccggc aagaccggca ctgccgagca
     5881 gggcaacggg ctcccggcgc acgcgtggtt cacgtcgttc gcgcccgccg acaaccccca
     5941 gatcgccgtc gcggtcatcg tcgaggatgg cggcaacgcg ggcgacgagg ccggcggcgg
     6001 ccgcacggcc ggcccgatcg ccaaggccgt catggaagcg gtgatcaccc gatgagcccg
     6061 atgactccag gcctgggcga cgtcctcggc ggccgctaca ccttgaccga ccggatcgcc
     6121 gccggcggga tgggcgacgt ctgggccgcc accgaccgcg tccttggccg caccgtcgcc
     6181 atcaagatca tgcgtcccaa caccaccgac gaaggcaact tcgccgcgcg cttccgggac
     6241 gaggcacggc ataccgcggc gttgtcccac cccaacatcg cggcggtcta cgactacggc
     6301 gaagacgacg gcacggcata cctcgtcatg gagttggtcc ccgggctgcc gctgtcgagc
     6361 atgatcgccg aggggccgat gccccccgag caggt
//
LOCUS       DAORPW010000126         6325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3288_length_6325_cov_814.132855, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000126 DAORPW010000000
VERSION     DAORPW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3288_length_6325_cov_814.132855"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLB86_09705"
     CDS             complement(<1..197)
                     /locus_tag="PLB86_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65450.1"
                     /translation="MTTNDTTTDPAGFHHGIVPEVLHEQPASKPVKFYQVGSFAVGNR
                     LLPLEVRSVQSDPNRTNSLTSG"
     gene            complement(214..1467)
                     /locus_tag="PLB86_09710"
     CDS             complement(214..1467)
                     /locus_tag="PLB86_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65451.1"
                     /translation="MRTQIEGSLAVAQAVAACRPQVICAYPISPQTHIVEGLSAIVKR
                     GELPGCEYLNVESEFAAMSGAIGASAAGARSYTATASQGLLYMVEAVYNASGLGLPIV
                     MTVANRAIGAPINIWNDHTDSMSQRDSGWIQLYAETNQEAVDLHVQAFRIAEELSLPV
                     MVCMDGFILTHAVEEVDVPEAADVARFLPPFEPRQVLDPANPVSIGAMVGPEAFTEVR
                     YLAHERQMQALDLIPAVAAEFQAVFGRDSGGLLHTYEIEDAETVVIALGSVLGTIKDV
                     VDERRARGEKIGVLGITSFRPFPLKAVEAAIGNAKRFVCLEKAFSVGIGGIVSSHVRM
                     AMSSHPIKNYTVVAGLGGRPILKTSLNDMLDQATTDSLEPLTFLDLDTDLVEREIARN
                     RAERRSGPAAENMLRDLGGSRPVTV"
     gene            complement(1478..2053)
                     /locus_tag="PLB86_09715"
     CDS             complement(1478..2053)
                     /locus_tag="PLB86_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRC65452.1"
                     /translation="MFEVRIHGRGGQGVVTAAELLSVAAFDEGRHAQAFPSFGSERTG
                     APVVAFCRVADQEIRVREPVVTPDALVVQDSTLLHQVDVFAGLKADGYALINSPLTPS
                     ELGLDELAARLGVGRLLTIPATDIAREHIGRPVPNLVMLGGLAAFTGLVHLPALLEAM
                     QDHFPGALGAKNVAAATAAYELVQSLVPSHT"
     gene            2312..3817
                     /locus_tag="PLB86_09720"
     CDS             2312..3817
                     /locus_tag="PLB86_09720"
                     /EC_number="2.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA carboxytransferase subunit 5S"
                     /protein_id="HRC65453.1"
                     /translation="MSKKIDVTELALRDAHQSLFATRMALEDMVPICEDIDQAGYWSV
                     ECWGGATFDACIRFLNEDPWERLRTFRSLLPNSKLQMLLRGQNLLGYRHYEDDVVDRF
                     VGKAVENGMDVFRTFDALNDVRNVKRAIAAVKREGAHAQGTLCYTVSELHTVEGYVQM
                     ARDLMDLGCDSLCLKDMAALLQPQPAYDIVKAIKDDLGQDVRIHIHCHATTGVTLVSL
                     MKGIEAGADCVDAAISSMSLGPGHNPTESLVEMLPGTGFHTELDEGRLIKIKDYFAGI
                     RPKYAEFASNIVGVETEIFKSQIPGGMISNMESQLKAQGAAHRIKEVLEEVPRVRADA
                     GYPPLVTPSSQIVGTQAVFNVMMGRYKVLTGEFADVMLGYYGSTLGTPNPEVVEIARA
                     QAKKDPITCRPADLLPAEWEKDREEAGALRGFNGSDEDVLTYAMFPKVAPTFFGTRDE
                     GPKNVGKDPDTLAAEKAATEAGAQPGQPVRTPIRYKVIVNGAPHSVQVQPS"
     gene            3843..5381
                     /locus_tag="PLB86_09725"
     CDS             3843..5381
                     /locus_tag="PLB86_09725"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRC65454.1"
                     /translation="MQEKIDQLHQRRASLAAAGGEQRVAKQHEQGKLTARERIEELLD
                     EHSFQESAVYAVHRANRFGMSEKDVPSDGVVVGQGTVLGRLVHVASQDFTILGGTIGE
                     LHADKIAQAANMALRTGSPFVCINDSGGARVQEGVDALNGCGHIFYANVLLSGVVPQI
                     SILAGPCAGAAAYSPALTDFIIQTKQAQMYITGPDVIKQVTGEEVSHEDLGGAAVHAG
                     RSGVVHFVAEDDAQAALIAKKLLSFLPQNNTEDPPEIEHDGNADDDPFLDSVIPDDYR
                     KPYDVRDVIARVVDDADFLEVHASWAANAVVGFGRIVGRTVGVIANQPNQLSGVLDIN
                     ASDKIARFVRFCNAFNIPLLTFVDTPGFMPGVDQEYNGIIRHGAKVLFAYSAATVPKV
                     TVVLRKAFGGAYIAMCARALGADKVFAWPSAEIAVMGAEGAAGIVFRKEISQADDPAG
                     KRQELIDEYRGTFANPYVASEHGLVDDVIVPAETRRFVAQALESLTTKRDMRPPKKHG
                     LMPM"
     gene            5426..5704
                     /locus_tag="PLB86_09730"
     CDS             5426..5704
                     /locus_tag="PLB86_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65455.1"
                     /translation="MTSTPMDVARLADLVEEMQRRITKLEKQLAEAQGPQGPVPIDTI
                     LAISAAVAAYLGKKATVRQIHYSHTNSWARMGRSNIHASHTFTHTRSH"
     gene            5728..6111
                     /locus_tag="PLB86_09735"
     CDS             5728..6111
                     /locus_tag="PLB86_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRC65456.1"
                     /translation="MRLKVTVDGVEYDVLVDVEAEPKPALGGFVFSSTGTATMPRSGP
                     AASAGEAAGGGGGENALRAPIAGTVLQVNVQPGAEVKEGDTLIILEAMKMETEITAPV
                     AGTVKSVPVQVGSPVQGGDILVDFE"
BASE COUNT         1095 a         2126 c         2132 g          972 t
ORIGIN      
        1 ccggaggtga gggagttggt gcggttgggg tcggactgga ccgagcggac ctccaacggc
       61 agcagtcggt ttccgacggc gaaggagccc acctggtaga acttcaccgg cttgctcgcc
      121 ggctgctcat gcagcacttc gggaacaatc ccgtggtgga aacccgccgg gtcggtcgtg
      181 gtgtcgttgg tcgtcatgtc agcgtcctcc tggtcagacc gtcacggggc gggagccacc
      241 caggtcgcgg agcatgttct cggcggccgg tcccgagcgg cgctccgccc ggttgcgggc
      301 gatttcgcgc tcgacgaggt cggtgtccag gtcgaggaag gtcagcggct ccaacgagtc
      361 ggtggtcgcc tggtcgagca tgtcgttcag cgaggtcttg aggatcggtc gaccgccgag
      421 gccggcgacg accgtgtagt tcttgatcgg gtgtgaggac atcgccatcc gcacgtgcga
      481 ggagacgatg ccgccgatgc ccaccgagaa ggccttctcg aggcagacga agcgcttggc
      541 gttgccgatc gcggcctcga cggccttgag cgggaacggc cggaacgagg tgatcccgag
      601 gacgccgatc ttctcgcctc gggcccgtcg ctcgtcgacg acgtccttga tcgtgcccag
      661 caccgacccg agcgcgatga cgacggtctc ggcgtcctcg atctcgtagg tgtgcagcag
      721 cccgccggag tcgcggccga agaccgcctg gaactcggca gccaccgcgg ggatgaggtc
      781 aagggcctgc atctggcgct cgtgggcgag gtagcggacc tcggtgaacg cctcggggcc
      841 gaccatcgca ccgatcgaca cggggttggc cgggtcgagc acctggcgcg gctcgaacgg
      901 cggcaggaac cgcgcgacgt cggcggcctc gggcacgtcg acctcttcga ccgcgtgggt
      961 gaggatgaag ccgtccatgc agaccatgac gggcagggac agctcttcgg cgatccggaa
     1021 ggcctgcacg tgcaggtcga ccgcctcctg gttggtctcg gcatacagct ggatccagcc
     1081 ggagtcgcgc tgactcatcg agtcggtgtg gtcgttccag atgttgatcg gcgcgccgat
     1141 ggcccggttg gcgaccgtca tgacgatcgg caacccgagc ccgctggcgt tgtagacggc
     1201 ctccaccatg tagagcaggc cctggctggc ggtggccgtg taggaccgcg ccccggctgc
     1261 cgaggccccg atggccccag acatcgcagc gaactcgctc tcgacgttga ggtattcgca
     1321 gccgggcaac tcgccacgct tgacgatggc cgagaggcct tcgacgatgt gcgtctgggg
     1381 cgagatgggg taggcacaga tgacctgcgg ccgacacgcg gcgaccgcct gggcaaccgc
     1441 gagcgagccc tcgatctggg tgcgcaacgg acgtctccta ggtgtgggac ggaacgaggg
     1501 actgaacgag ctcgtatgcc gcggtcgcgg ctgccacgtt cttggcaccg agggcgccgg
     1561 ggaagtgatc ctgcatcgcc tcaaggagcg cgggcaggtg gaccaggccg gtgaacgcgg
     1621 ccaggcctcc gagcatcacg aggttgggca ccggtcgccc gatgtgctcg cgtgcgatgt
     1681 cggtcgccgg aatggtgagg aggcggccga cgccgagtcg ggccgccagc tcatccaggc
     1741 cgagttcgct cggcgtgagg ggcgagttga tgagtgcgta gccgtccgcc ttgaggccgg
     1801 cgaagacgtc gacctggtgc agcagggtcg agtcctgcac cacgagggcg tcgggagtca
     1861 cgacgggctc ccggacgcgg atctcctggt cggcgacgcg acagaacgcg acgacagggg
     1921 ctccggtgcg ctcggaaccg aaactgggga aggcctgggc atggcgacct tcatcgaatg
     1981 cggcaacgga cagcaactcg gcggcagtga ccacgccctg tccgcctcgg ccgtggatgc
     2041 ggacctcgaa catgcgttga acgtatgccg cggatcacga tgaggcctag ggaccaatgg
     2101 tcctcacttc gaccgccatg ctcagccgca ggtgaacatc ggatggacac ctgccgcggg
     2161 gcggcatacc gcatggggaa ggtgaccaaa gccgggccag aggtcccagg atcgagtccg
     2221 ttttgcgtgc cacattgacg ggcacgaggg tatgccgctc cccgcatgtc gcggcgcccg
     2281 tcgactgccg ccgtcggaag gtgtcgccac gatgagcaag aagatcgacg tcacggagct
     2341 cgccctgcgc gatgcccacc agagcctgtt cgccacccgg atggccttgg aagacatggt
     2401 cccgatctgc gaggacatcg accaagccgg gtattggagc gtcgagtgct ggggcggcgc
     2461 gaccttcgac gcctgcatcc ggttcctcaa cgaagacccg tgggagcggc tgcgcacttt
     2521 ccgcagcctg ctgcccaact ccaagctgca gatgctcctg cgcgggcaga acctcctcgg
     2581 ctaccgccac tacgaggacg acgtcgtcga ccgcttcgtc ggcaaggccg tcgagaacgg
     2641 catggacgtc ttccgcacgt tcgacgccct caacgacgtg cgcaacgtca agcgcgccat
     2701 cgctgcggtc aagcgtgaag gggcgcatgc ccagggcact ctctgctaca ccgtgagcga
     2761 gttgcacacg gtcgagggct acgtccagat ggcccgtgac ctcatggatc tcggatgcga
     2821 ctcgctgtgc ctcaaggaca tggcggcgct gctgcagccg caaccggcat acgacatcgt
     2881 caaggcgatc aaggacgacc tcgggcagga cgtccgcatc cacatccact gccacgcgac
     2941 gaccggcgtc accctcgtgt cgttgatgaa gggcatcgag gccggcgccg actgcgtcga
     3001 tgccgcgatc tcctcgatga gcctcggccc gggtcacaac cccaccgaga gcctcgtcga
     3061 gatgctgccc ggcaccgggt tccacaccga gctcgacgag ggtcgactca tcaagatcaa
     3121 ggactacttc gccgggatcc gcccgaagta cgccgagttc gcgtcgaaca tcgtcggtgt
     3181 cgagaccgag atcttcaaga gccagatccc cggcgggatg atctccaaca tggagagcca
     3241 gctcaaggct caaggggctg cccaccggat caaggaggtg ctcgaggagg ttccgcgggt
     3301 gcgcgccgac gccggctacc cgcccctggt caccccgtcg agccagatcg tcggcaccca
     3361 ggcggtcttc aacgtcatga tgggccgcta caaggtcctc accggtgagt tcgccgacgt
     3421 catgctcggc tactacggca gcacgctcgg cacgccgaac cccgaggtcg tcgagatcgc
     3481 ccgggcgcag gccaagaagg acccgatcac ctgccggccg gctgacctgc tcccggccga
     3541 gtgggagaag gaccgcgagg aggccggcgc gctgcgcggc ttcaacggtt ccgacgagga
     3601 cgtgctcacc tacgcgatgt tcccgaaggt cgctccgacc ttcttcggca cccgtgacga
     3661 gggccccaag aacgtcggca aggaccccga cacgctcgcc gccgagaagg ctgccaccga
     3721 ggccggcgcc cagcccggcc agccggttcg tacccccatc cgctacaagg tcatcgtcaa
     3781 cggcgccccc cacagcgtcc aagtccagcc gtcctgaaag gtcagccgtg gcagcgcaga
     3841 gcatgcagga gaagatcgac caactgcacc agcgtcgggc atcgctcgcc gccgccggag
     3901 gcgagcagcg ggtcgccaag cagcacgagc agggcaagct caccgcccgt gagcggatcg
     3961 aggagctcct ggacgagcac tcgttccagg agtcggcggt gtatgccgtg caccgcgcca
     4021 accgcttcgg catgtccgag aaggacgttc cgagcgatgg cgtcgtcgtc ggtcagggca
     4081 cggtgctcgg gcggctcgtc catgtggcca gccaggactt caccatcctc ggcggaacca
     4141 tcggtgagtt gcacgccgac aagatcgcgc aggccgccaa catggcgttg cgcaccgggt
     4201 cgccgttcgt gtgcatcaac gactcgggtg gtgcgcgggt ccaggagggc gtcgacgctc
     4261 tcaacggctg tggccacatc ttctacgcca acgtgctgct ctcgggtgtg gtcccgcaga
     4321 tctcgatcct ggccggcccc tgcgccgggg ccgcggcata ctcgccggcc ttgacggact
     4381 tcatcatcca gaccaagcaa gcgcagatgt acatcaccgg ccctgacgtc atcaagcagg
     4441 tcaccggtga ggaggtcagc cacgaggacc tcggcggcgc ggcggtccac gccggccgct
     4501 cgggggtcgt tcacttcgtc gccgaggacg acgctcaggc ggcgttgatc gccaagaagc
     4561 tcctgagctt cctcccgcag aacaacaccg aggacccgcc cgagatcgag cacgacggca
     4621 acgccgacga cgacccgttc ctcgactccg tcatccctga tgactaccgc aagccctacg
     4681 acgtgcgtga cgtcatcgcc cgcgtcgtcg acgacgcgga cttcctcgag gtccacgcca
     4741 gctgggccgc caacgccgtc gtcggcttcg gccggatcgt tgggcgcacg gtcggcgtca
     4801 tcgccaacca gcccaaccag ctctccggtg tcctggacat caacgcgtcc gacaagatcg
     4861 ctcgcttcgt gcggttctgc aacgccttca acatcccgct gctcaccttc gtcgacaccc
     4921 ccggcttcat gcctggcgtc gatcaggaat acaacgggat catccggcac ggcgccaagg
     4981 tgttgttcgc ctactcggcg gccaccgttc cgaaggtgac ggtcgtcctg cgcaaggcct
     5041 tcggcggtgc ctacatcgcc atgtgcgcgc gagcgctggg tgccgacaag gtgttcgcct
     5101 ggccgtcggc cgagatcgcc gtcatgggcg ccgagggtgc cgccggtatc gtcttccgca
     5161 aggagatcag ccaggctgac gacccggccg gcaagcgcca ggaactcatc gacgaatacc
     5221 gcggcacctt cgccaacccg tatgtggcga gcgagcacgg tctggtcgat gacgtcatcg
     5281 ttccggccga gacccgccgg ttcgtggccc aggcgttgga gtccttgacg accaagcgcg
     5341 acatgcgtcc gcccaagaag cacggcctca tgccgatgtg acctggcgac cgacgacgac
     5401 gaacagataa ccacgagagg ttcttatgac aagcaccccg atggatgtcg ctcgcttggc
     5461 cgacctggtc gaggagatgc agcgacggat caccaagctg gagaagcagc tggccgaggc
     5521 gcaggggccc caggggccgg tgccgatcga cacgatcctc gccatctccg ccgcggtcgc
     5581 ggcctacctg ggcaagaagg cgaccgttcg ccagatccac tacagccaca ccaactcctg
     5641 ggcacggatg ggccgctcca acatccacgc ctcgcacacc ttcacccaca cccggtccca
     5701 ctgacccgcg actgacaagg aacgcccatg cgactcaagg tgaccgttga cggggtcgaa
     5761 tacgacgtgc tcgtcgacgt cgaggccgag ccgaagcccg cgttgggcgg cttcgtcttc
     5821 tcctccaccg gcacggcgac gatgccgcgc tccgggccgg cggcatctgc cggtgaggcg
     5881 gctgggggtg gcggcggcga gaatgcactg cgggcgccga tcgccggcac cgtgctgcag
     5941 gtcaacgtcc agccgggcgc cgaggtcaag gagggcgaca ccctcatcat cctcgaggcc
     6001 atgaagatgg agacggagat caccgcgccc gtggcgggca cagtgaagtc ggtcccggtc
     6061 caggtcggct cgcccgttca gggcggggac atcctcgtcg acttcgagta gcccaccagc
     6121 ccactcaccc acccgcgcgg ccggtccgcg ccgacagtat gccgcccgct cacctgcggg
     6181 cggtatacct gtgtttacgg gtcaccgcgg agggcgcagc caaatctgag gttgcttggc
     6241 agtttgggtc atttgcccac gcgagtgccc ggcagggtca tagcgtcgaa gcaacggagt
     6301 cggtcgtagg ggcttggcct gggtt
//
LOCUS       DAORPW010000127         6288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3320_length_6288_cov_753.857051, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000127 DAORPW010000000
VERSION     DAORPW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3320_length_6288_cov_753.857051"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..393
                     /locus_tag="PLB86_09740"
     CDS             94..393
                     /locus_tag="PLB86_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65457.1"
                     /translation="MTVLVQIRDVDASVRDELKARAARSGVSFNSYLRDLLAVAAARP
                     TREEVLARIQARTGGATASATELIRAERDARERPPTARASGRTAGSRRGHSGAAP"
     gene            390..794
                     /locus_tag="PLB86_09745"
     CDS             390..794
                     /locus_tag="PLB86_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRC65458.1"
                     /translation="MTIRSGDVVLDASVVVDLLVAPDRGRLGSALAASAWHAPVHLDV
                     EVVSALRGLVLGRHLSAERAVDALVDFADLEITRWAPDLAVTRRIFALRDRLSAYDAA
                     YLVCAEGIAAPLATRDQRLARAVADLVDVTVL"
     gene            complement(903..2669)
                     /locus_tag="PLB86_09750"
     CDS             complement(903..2669)
                     /locus_tag="PLB86_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65459.1"
                     /translation="MSVSAGVPSARRTLPVATLASVRAQTAALLREHRGALIEVVGLH
                     AIAAVAGLAAPFVVGRLVDEVTAGTAYESVDRQALWLAGAILAQTVLVWAARRSSFRL
                     GERVFAQLREQFMTRVMALPLSTVERAGTGDLVSRTTNDVEALSHVVRFGIPSIFVAV
                     VTLAITVAAAVATGWLVALPVVVGVPIIWLSTRRYLRFAPDGYLRERATYAVMNGVVT
                     ETVDGARTVDALGLSARRRRRMDEALRASFDAEMYTLRLRLIWFPQVEFAYLLPVAAT
                     LVWGGWLVSQGHASIGAVTTVALYVHQLANPLDDLLMWLDEIQVGATSLARVIGIADV
                     PPDREATGETPVDAHLVVDDVSYAYRAGRDVLRGVSLDLAPGERLAIVGPSGAGKSTL
                     GRLMAGIDGPRLGRVDVGGVRLVDLDLDRLRGEVALVTQEHHVFVGTLADNLLLARPS
                     ASRDELLDALTAVDGREWALGLADGLDTRVGSGAHSLTTAQSQQLALARLVLADPHTL
                     VLDEATSLLDPRAARHLERSLAAVVTGRTVVAIAHRLHTAHDADRVAVVDGGLVTEIG
                     SHDELIAAGGAYAALWRSWRDE"
     gene            complement(2669..4417)
                     /locus_tag="PLB86_09755"
     CDS             complement(2669..4417)
                     /locus_tag="PLB86_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65460.1"
                     /translation="MRPLPYDHPGTPVLTSPMAFLGWVGRRQWRTLLGGVTFGVIWMV
                     AQALIPATLAKAIDEGIIPGDTGALLRWSGALLGLAAVTALAGAARHQLAVANWLRAA
                     FRSVQLIGWHSADTGEALPRDTPTGDVVATVASDSMRLGGAYDTTARFAGAIVSYIVV
                     AVILLRASMPLGLLVLIGVPVLVGLLSFIVRPLQRRQAQQREESGRLIGLGADTVAGL
                     RVLRGIGGETTFLARYAAQSQQVREVGYRVAGVQAALDAAQVLLPGIFVVLVTWLGAR
                     FAIDGRITPGQLVAFYGYSAFLVTPLRTATEFVDRLTRAHIGARKIIRVLKVEPDHVD
                     HPDVVTPGATEPPADSALRDERSGVVVEPGQFLAVVSGRPEESAALADRLGRFGTDVE
                     GVTLGGIPLTHLPLATVRRRILVSETDPRLFTGVLRDELDPWGAHSDEEILAALTIAS
                     GEDVLEALADGLDSEVEERGRSFSGGQRQRLALTRALLANAPILVLVEPTSAVDAHTE
                     ARIADRLATFRAGRTTVVMTASPLVLDRADRVLLLQDDAILAEGTHHELMHRRDGASA
                     AYRAVVVRGADEEETR"
     gene            4498..5325
                     /locus_tag="PLB86_09760"
     CDS             4498..5325
                     /locus_tag="PLB86_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRC65461.1"
                     /translation="MLRTALTPRWLVWLVVVLGLGAGFVWLGLWQMNVAREDAAAEVL
                     REAAARPVLELTATIQPHAAFPADGSNQRVQAVGRFEPARQVLVADRRLAGADGFWVV
                     QPFVVQETGARIPVVRGFVRSLAAADAVAANSAAGSGATEPLTLVGSLAPTESSSAGK
                     KRLPDGQIGSIDVGALLNDWGGEVYNAFLFDISETPDQGVAGTTGASASGGMERVPPP
                     DLPSGLTLRNAAYALQWWVFAAFGLWMWVKMVRDDYRRHQPVPPLGAGPTADVGATQ"
     gene            5328..5672
                     /locus_tag="PLB86_09765"
     CDS             5328..5672
                     /locus_tag="PLB86_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HRC65462.1"
                     /translation="MYPTNLAKPLQIRRALGIYRVLAIVAGIALFILVVEMVMKYGMH
                     QENWFTKNWSYIHGFIYMAYAASIANLGLKLAWPMMRIVVNLLSGFVPLAPFFAERKI
                     TAETNGILARVY"
     gene            5733..>6288
                     /gene="guaA"
                     /locus_tag="PLB86_09770"
     CDS             5733..>6288
                     /gene="guaA"
                     /locus_tag="PLB86_09770"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRC65463.1"
                     /translation="MSETLQSRPVLVVDFGAQYAQLIARRVREAKVYSEVVPHTMSVA
                     AMLAKNPAAVILSGGPASVYAAGAPSLDPALLAAGVPVFGMCYGFQAMVQALGGTVAH
                     TGTGEYGHTDAVVTPDGSLLFDGQPKEQSVWMSHGDSVTEAPAGMAVTATTPGATVAA
                     FEDADRRLFGVQWHPEVLHTAYGQR"
BASE COUNT          965 a         2221 c         2219 g          883 t
ORIGIN      
        1 atacatcgat gcggagttag acctcctgct cgccgccatc cgcgcctgac ccggcaggtt
       61 ggggatgcat gagtttcaat ctattctgta tgcatgaccg tcctggtgca gatccgcgat
      121 gtcgatgcgt cggtgcgtga cgagttgaag gcccgcgccg cccgctcagg cgtctcgttc
      181 aacagctacc tgcgtgacct gctcgcggtg gcagcggcgc gtccgacgcg ggaggaagtt
      241 ctggcgcgga tccaggcccg gaccgggggg gcgaccgcct cggcaacgga actcatccgg
      301 gccgagcggg atgcccggga acggccaccc acggcgcgag cgtccgggcg aaccgccggc
      361 tctcgacgcg gccattcagg cgcggccccg tgacgattcg gtccggcgat gtcgtcctgg
      421 atgcttccgt cgtcgtcgac ctcttggtgg cgccagaccg cgggcggctg ggctcggcgt
      481 tagccgcctc cgcgtggcac gcgccggtgc acctcgacgt cgaggtcgtc tcggcattgc
      541 gtggactggt gctgggtcgg catctcagcg ccgagcgggc agtggacgct ctcgtggact
      601 tcgccgactt ggagatcact cgctgggctc ccgacctggc agttacccga cggatcttcg
      661 ccctgcgtga tcggctcagt gcctacgacg cggcatacct cgtctgcgcc gaagggatcg
      721 ccgcgccatt ggccacgcgc gaccagcggt tggctcgcgc cgtggctgac ctcgtcgacg
      781 tcaccgtcct ctagcccctc ccgccaccga ccccgtccca ccacccggtc gaggtattcc
      841 gccatcgtca gaagatggcc gaatacctcg acccggcggg ggaggggcaa gcagtgaccc
      901 ggctactcgt cgcgccagga gcgccagagg gcggcatacg ccccaccggc ggcgatgagc
      961 tcgtcgtgcg agccgatctc ggtcaccaac ccgccatcga ccacagccac ccggtcggcg
     1021 tcgtgagccg tgtgcaggcg gtgggcgatc gccacgacag tgcgcccggt aacaaccgcg
     1081 gccagcgagc gctccaggtg ccgcgccgcc cgagggtcta gcagcgaggt cgcctcgtcg
     1141 agcaccaacg tgtgcgggtc ggcgagcacc agccgagcca gcgccaactg ctgcgactgg
     1201 gccgtcgtca acgagtgagc accggacccg acccgggtgt ccagcccgtc ggccagccct
     1261 aacgcccact cgcgcccgtc gacggcggtc aacgcgtcca acaactcgtc gcgactagcc
     1321 gagggccggg ccagcagcag gttgtccgcc aacgtcccga cgaacacgtg gtgctcctgc
     1381 gtcaccaagg ccacctcacc ccgcaaccgg tcgaggtcga gatcgaccaa cctcaccccg
     1441 ccgacatcga cccgaccgag ccgcggcccg tcgatccccg ccatgagccg cccgagcgtc
     1501 gacttgccgg cccccgacgg cccgacgatc gccagtcgct ccccgggcgc cagatccagc
     1561 gacactccgc gcagcacgtc gcgcccggcg cgataggcgt aggacacgtc gtcgacgacg
     1621 agatgcgcat ccaccggcgt ctcgccggtc gcctcacggt ccgggggcac gtcggcgatc
     1681 ccgatgaccc gggcgagcga cgtcgcccca acctggatct cgtccagcca catgagcagg
     1741 tcgtccaggg ggttggccag ttggtggaca tagagcgcca ccgtcgtcac ggccccgatg
     1801 ctcgcgtgcc cctgcgagac cagccaaccg ccccagacca gggtcgccgc caccggcaac
     1861 agataggcga actcgacctg ggggaaccag atcaaccgca gccgcagggt gtacatctcg
     1921 gcgtcgaacg atgctcgcaa cgcctcgtcc atccggcgcc gacgccgggc cgacaacccg
     1981 agcgcatcga cggtccgagc gccgtcgaca gtctcggtga cgacgccgtt catcacggca
     2041 tacgtcgcgc gctcgcgcag ataaccgtcg ggggcaaagc gcagatagcg ccgggtcgag
     2101 agccagatga tcggcacgcc gacgacgacg ggcagcgcga cgagccaccc ggtggccacc
     2161 gcggccgcga cagtgatcgc cagcgtgacc accgcgacga agatcgacgg gatgccgaac
     2221 cggacgacgt ggctgagcgc ctcaacgtcg ttggtcgtcc gcgatacgag gtcaccggtg
     2281 cccgcccgct cgacggtcga gagcggcagc gccatcaccc gcgtcatgaa ctgctcacgc
     2341 aactgggcga agacccgctc gccgagccgg aacgacgacc gccgcgccgc ccacacgagc
     2401 actgtctggg cgaggatcgc accggccagc cacagggcct gccggtcgac gctctcgtat
     2461 gccgtgcccg cagtgacctc gtcgacgagc cgccccacga cgaacggcgc cgccagccct
     2521 gcgaccgcgg cgatggcatg cagcccgacg acctcgatga gcgcgccgcg gtgctcgcgc
     2581 aacagcgccg ccgtctgggc acgcaccgag gccagcgtgg ccacgggcag agtgcgtcgg
     2641 gcgctcggga ctccggcgga gacggacatc agcgggtctc ctcctcgtcg gcgccgcgca
     2701 cgaccaccgc acggtatgcc gcgctcgcac cgtcgcgtcg gtgcatgagc tcgtggtggg
     2761 tcccctcagc gaggatggcg tcgtcctgca gcagcagaac gcggtcggcc cgatcgagga
     2821 cgagcgggct ggcggtcatg acgaccgtcg tccgcccggc ccggaaggtc gccagccggt
     2881 cggcgatgcg ggcctcggtg tgggcgtcga cggcactggt cggctcgacg agcacgagga
     2941 tcggcgcgtt ggccagtagc gcccgggtca gcgccaggcg ttggcgttgg ccgccggaga
     3001 acgaccggcc gcgctcttcg acctcgctgt ccaggccgtc agcgagcgcc tccaggacgt
     3061 cctcgccgct ggcgatcgtc agggccgcga ggatctcctc gtcggagtgg gcgccccacg
     3121 ggtcgagctc gtcgcgcagc accccggtga acaggcgcgg gtcggtctcg ctcacgagga
     3181 tccggcgccg caccgtcgcg agcggcaagt gagtgagggg tatgccgccc agcgtcaccc
     3241 cctcgacgtc ggttccgaag cgccccaacc ggtcggcgag cgcggccgac tcctcggggc
     3301 gtccgctgac caccgcgagg aactgtccgg gctcgacgac gacgccgctg cgttcgtcgc
     3361 gcagggcgga gtcggcggga ggctcggtgg cgccaggagt cacgacatcg gggtgatcga
     3421 cgtgatcagg ctcgaccttg agcacccgga tgatcttgcg ggcgccgatg tgggcgcggg
     3481 tcaaccggtc gacgaactcg gtcgcggtgc gcagcggggt gacgaggaag gccgagtagc
     3541 cgtagaacgc aaccaactga ccgggcgtga tccggccgtc gatcgcgaac cgagccccga
     3601 gccacgtgac gaggacaacg aagatgcccg gcaggagcac ctgggccgca tcgagggcag
     3661 cctggacgcc ggccacccga tagccgacct cgcgcacctg ctggctctgg gcggcatacc
     3721 gggcaaggaa cgtcgtctcg cccccgatgc cgcgcagcac ccgcaaccca gccacggtgt
     3781 cggccccgag cccgatcagc cgaccggact cctcgcgctg ctgggcctgg cggcgctgca
     3841 gcggccggac gatgaaggac agcaaaccga cgaggaccgg cacgccgatg aggacaagca
     3901 ggccgagcgg catcgaggcg cgaaggagga tgacggcgac gacgatgtag gacacgatcg
     3961 ctccggcgaa ccgcgcggtg gtgtcgtagg ccccacccag ccgcatcgag tccgaggcga
     4021 ctgtggcgac cacgtcgccg gtgggggtgt cacgaggcag cgcctcgccc gtgtcggcgc
     4081 tgtgccagcc gatgagttgc acggaccgga acgccgcccg cagccagttg gcgaccgcga
     4141 gttggtggcg ggccgcgccg gcgagcgccg tgacggcagc cagacccaag agggcacccg
     4201 accaacgcag caaggccccg gtgtcgcccg gaatgatccc ttcgtcgatg gccttggcga
     4261 gggtcgccgg gatcagcgcc tgggccacca tccagatgac accgaaggtc actccgccca
     4321 ggagggtgcg ccactgccgc cggcccaccc agccaaggaa ggccatcggg ctggtgagca
     4381 ccggggtccc gggatggtcg tagggcagag ggcgcacaag gatccacgct acccgcgccg
     4441 ccagaccgct tcaccccgtt ttctccgggg tatgccgcgc gccgtagcct gggaggcgtg
     4501 ctgcggaccg ccctcacccc tcgctggttg gtgtggcttg tcgtggtgct ggggctgggc
     4561 gccgggttcg tctggctcgg gctgtggcag atgaacgtcg cccgcgagga cgccgccgcg
     4621 gaggtcctgc gcgaggccgc ggcccgcccg gtgctcgaat tgaccgcgac gatccagccg
     4681 catgcggcgt tccctgccga tgggtccaac caacgggtgc aggccgtggg ccgtttcgag
     4741 ccggcccgcc aggtgctcgt cgccgaccgt cggctggccg gggcggacgg gttctgggtc
     4801 gtgcagccgt tcgtcgtgca agagaccggt gcgcggatcc ccgtggtgcg cggcttcgtt
     4861 cgatcgctcg ctgcggccga cgccgtggcg gcgaactccg cggctggttc gggcgctact
     4921 gagccgctca ccctcgtggg gagcctggcc cccaccgagt cgtcgtccgc tggcaagaag
     4981 cgtctgcccg acgggcagat cggatcgatc gacgtcggcg ccctgctcaa cgactggggc
     5041 ggcgaggtct acaacgcctt cctcttcgac atcagcgaga cgccggacca aggggtggcg
     5101 gggaccacgg gagcctcggc atcgggcggg atggagcggg tgccgccccc cgacctgccg
     5161 tccgggctga ccctgcgcaa cgcggcatac gccctgcagt ggtgggtctt tgcggcgttc
     5221 ggcctgtgga tgtgggtgaa gatggtgcgc gacgactacc gtcgtcatca gccggtgcct
     5281 ccgctgggcg ctgggccgac cgccgatgta ggagccacgc agtgagcatg tatccgacca
     5341 acctggccaa accgttgcag atccgccggg cgctggggat ctaccgggtg ctcgccatcg
     5401 tcgccggcat cgcgttgttc atcctcgtcg tcgagatggt catgaagtac gggatgcacc
     5461 aggagaactg gttcacgaag aactggtcct acatccacgg cttcatctac atggcgtatg
     5521 ccgcgtcgat cgccaatctc ggcctcaaac tcgcctggcc gatgatgcgg atcgtcgtca
     5581 acctgctcag cgggttcgtg ccgctggcgc cgttcttcgc cgagcgcaag atcacggcgg
     5641 agaccaacgg catcctcgct cgggtctact gagcgcgacg tccaccgagg cgaccgccac
     5701 gcggggggcc cgcgcctcgg taggcttggc gggtgagtga gacgttgcag tcccggccgg
     5761 tccttgtcgt cgatttcggc gcgcagtatg cgcaactcat cgcgcgccgc gtccgtgagg
     5821 cgaaggtcta cagcgaggtt gtcccgcaca cgatgtcggt ggcggcgatg ctcgccaaga
     5881 atcccgctgc ggtcatcctc tccggcggcc ccgcgagtgt gtatgccgcg ggcgcaccgt
     5941 cgcttgaccc cgccctgttg gcggccgggg tcccggtgtt cgggatgtgt tacggcttcc
     6001 aggcgatggt ccaggcgttg ggtggcacgg tcgcccacac ggggacgggt gagtatggcc
     6061 acaccgacgc ggtggtgacc ccggacggct cgctgctctt cgacgggcag ccgaaggagc
     6121 agtcggtgtg gatgtcgcac ggcgactcgg tgaccgaggc tccggccggg atggccgtga
     6181 cggcgacgac cccaggggcc acggtggcgg ccttcgagga cgcggaccgg cggttgttcg
     6241 gagtccagtg gcacccggag gtgctgcaca cggcatacgg gcagcgcg
//
LOCUS       DAORPW010000128         6249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3356_length_6249_cov_662.533581, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000128 DAORPW010000000
VERSION     DAORPW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3356_length_6249_cov_662.533581"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /locus_tag="PLB86_09775"
     CDS             complement(<1..1677)
                     /locus_tag="PLB86_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRC65464.1"
                     /translation="MRVPFFRPAAALAVGVLVVVGSLAACTSAPEPPTPEPVAAALAA
                     GLTSGDLNAVVFDGATPAAATDSVRNAVGALSSITRTVTVESVTRAADDANSATAKLR
                     WTWNLTGADGAWAYSVDAKLARAADQSWHVAWSPTLVEPKLTATERLALERRQAARGS
                     ILGANGEVLVGLKDVYRVGIDKGRISADQAQISATALATLMGTDATAYAKTVAASGPA
                     QFVPAITLRIDDPRIAGKTAAVQAIPGGAAMPGQAMLAPTATFARALLGTVGPATAEL
                     VSKSGGRIVAGDTSGLSGLQLRYDLPLSGQPGVVVLAKGTDSGGAASSRTLFTKAPVP
                     GVSLTTTLDRTAQDAAEQVLTGASGVASLVAIRPSTGEIVAVANSPATNGQAVATTGQ
                     AAPGSTFKIVTSLALLRAGLTPESPMSCTDTVTVDGRVFKNYDAYPASKLGQLPLRLA
                     LANSCNTAFISQRGTVSQDDLADAAAALGIGVAYDTGYSGFVGSIPREATQTEHGASM
                     IGQGRVTASALTMAIVMATIVHGSTLLPRLVDRPAATSPAPGGDADQSGSA"
     gene            complement(1759..2922)
                     /locus_tag="PLB86_09780"
     CDS             complement(1759..2922)
                     /locus_tag="PLB86_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC65465.1"
                     /translation="MPLDLHALTPDQLRARRTLKWTAYPADLLPMWVAEMDFPVCREV
                     TAALTRAVAAESFGYPSPVVHAELADALVEWQAQHHGWAIDPAGVHSVGDVMHGVDLA
                     IRYFSGPDDPVIIPTPVYMPFFDIVRLTGRPQVHVPMSWDGARWTFDLAAIDAAFAAG
                     GRTVLISSPHNPLGRVFDAAELAGLAAVVARHGGRVISDEIHAPLVFDRPHLPYAAAS
                     PEAADHSITVLSASKAWNLPGLKCAQVITTNAADTAAWKAMPYWETVGVSTLGMEASI
                     AAYREGGEWLAQVVATLAEHRDLVAQAVASWDGVTTVPNEGTYLQWLDFTGLGLDVEP
                     SAWLVEQARVLVNPGVPFGGEPLRFARLNFATTRSLLDEGLGRIEQAVRSRRP"
     assembly_gap    2950..3049
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3050..3978)
                     /locus_tag="PLB86_09785"
     CDS             complement(<3050..3978)
                     /locus_tag="PLB86_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC65466.1"
                     /translation="MPLRTVAVLLTERPLAFEFGVLAEVFGIDRTADGVPPFEFRVCA
                     ERPGEPLPMDNGVSLTVPFGLDGLRGADVVAVSSTQAKTFSEPVLEAVRQAHAAGATI
                     LATCSGGFVLAEAGLLDGRTATCHWYHAAEFAERYPATTVDATVLFVEDGTILTSAGT
                     AASIDACLHLVRREFGADVATRIARRMVVPPQRDGGQRQFVEQPIPTIAAESLEPLLT
                     WMMEHLEEDLPVRELARHAHLSERTFARRFLAETGTTPAKWIASQRLHHACRLLERTD
                     LPIEQVALRSGFGTAALLRDHFARNVGVPPADYR"
     gene            4073..4204
                     /locus_tag="PLB86_09790"
     CDS             4073..4204
                     /locus_tag="PLB86_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65467.1"
                     /translation="MDLILALATVIGALIVPMEITHRRTRSLPRLTQLERCGIRQWW"
     gene            complement(4254..4649)
                     /locus_tag="PLB86_09795"
     CDS             complement(4254..4649)
                     /locus_tag="PLB86_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC65468.1"
                     /translation="MSGWTGNIERATLDNDTFRTVVATGAHLQLVVMSLAPGEEIGLE
                     VHHDRDQFLRVEEGAAMVTLGPDDEVIEKTIALGEDWACIIPAGRWHNVINCGHGPLK
                     LYSIYSPPEHPDGTVHATKADADADEHEL"
     gene            4730..5866
                     /locus_tag="PLB86_09800"
     CDS             4730..5866
                     /locus_tag="PLB86_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2183 domain-containing protein"
                     /protein_id="HRC65469.1"
                     /translation="MARPHASAVVEDAWNSQRERVLRKRGWGTRVISHTGYGSTDFVR
                     VFARVVLGRALGDDDEPRQDATYGGMRSPYLRRRGWRAFFTAPAMNVPVEVHVGHHVA
                     HGRSDRSGHIDLTFRGHGMPPGWHEVTVSAQGCDPVQASVYIVAPDARFGLISDIDDT
                     VITTSLPRPMIAAWNTFFRHENARRVVPGMGPLYRTIMAEHPGAPIIFVSTGAWNTAP
                     TLTRFLKRNGFPIGPLLLTDWGPTNTGWFRSGQDHKRACLDRLAKDFPKIKWLLIGDD
                     GQHDPKIYQDFLEARPKRVAAVGIRQLTPAEQVLSHGLPVSTEELTGKAPRPRTVAPI
                     VYAPDGYGLLRLLRAAGLLAPSPAGEAGLGAVDGSATSDPLDSH"
     gene            5882..>6249
                     /locus_tag="PLB86_09805"
     CDS             5882..>6249
                     /locus_tag="PLB86_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HRC65470.1"
                     /translation="MPELPEVQALVDFLAERAVGQAVVAVELGSISVLKTYDPPPSAL
                     AGGPIDGVHRHGKFLDVDVDGIHLVFHLARAGWLRWSEAIPATVIRPGKSPIALRVRL
                     SDGSGFDLTEAGTKKSLAAYL"
BASE COUNT          898 a         2077 c         2260 g          914 t
ORIGIN      
        1 cgcggaaccc gactggtcgg catcgccgcc cggcgcgggc gaggtcgcag ccggccggtc
       61 gaccaatcga ggcaacagcg tcgagccgtg gacgatggtc gccatgacga tcgccatcgt
      121 cagggccgag gcggtgacgc ggccctgacc gatcatcgag gcgccatgct cggtctgcgt
      181 cgcctcgcgg gggatcgatc cgacgaagcc gctgtatccg gtgtcatagg cgaccccaat
      241 ccccaaggcg gctgccgcgt cggcgaggtc gtcctgcgag acggtccctc gctgcgagat
      301 gaaggcggtg ttgcacgagt tggccagcgc cagccgcaag gggagctgcc cgagtttcga
      361 ggcgggatag gcgtcgtagt tcttgaagac ccgcccgtcg acggtcaccg tgtcggtgca
      421 ggacatcggc gactcaggcg tcaggccggc gcgcagcagg gcgagggacg tgacgatctt
      481 gaaggtcgat ccgggcgcgg cctggcccgt cgtggcgacg gcctgcccat tggtggccgg
      541 tgagttcgcg acggcgacga tctcgccggt cgagggccgg atggcgacca ggctggccac
      601 cccggatgcg ccggtgagca cctgttcggc cgcgtcctga gccgtgcggt cgagggtggt
      661 cgtcagcgag acaccgggga cgggagcctt cgtgaacaag gtccgcgagc tggccgctcc
      721 gccgctgtca gtccccttag cgaggacgac cacgcccggt tggcccgaca gcggcaggtc
      781 gtagcgcaac tgcagcccgg acaggcccga ggtgtcgccg gcgacgatgc gcccgccgga
      841 cttgctgacc aactcggccg tcgcgggccc gacggtcccc agcagcgccc gtgcgaaggt
      901 cgccgtcggc gcgagcatcg cctggccagg catcgccgcg ccgcccggga tcgcttggac
      961 cgccgcggtt ttgcccgcaa tccgcgggtc gtcgatacgc agggtgatgg cgggcacgaa
     1021 ctgcgccggt cccgaggcgg ccacggtctt cgcgtatgcc gtggcgtcgg tccccatgag
     1081 cgtcgccaaa gcggtggctg agatctgcgc ctggtcggca ctgatccgac ccttgtcgat
     1141 gccgacccga tagacgtcct tgagcccgac gaggacctcg ccgttggcgc cgaggatcga
     1201 tccgcgagcg gcttggcgac gctcaagggc gagacgttcg gtggcggtga gcttgggttc
     1261 gacgagcgtg ggtgaccagg cgacgtgcca gctctggtcg gcggcgcggg ccagcttggc
     1321 gtcgacggag taggcccagg caccgtcggc gccggtgagg ttccaggtcc agcgcagctt
     1381 ggcagtggcc gagttggcgt cgtccgccgc tcgggtgacg ctctcgacgg tcacggtccg
     1441 ggtgatgctc gacagcgcgc cgacggcgtt gcgcacagag tcggtggctg ctgcgggcgt
     1501 ggcgccgtcg aagacgacgg cgttcaggtc tcccgaggtc aggccggcag ccagggcagc
     1561 cgcgacgggc tcgggtgtgg ggggctcggg agcactggtg caggccgcca aggagccgac
     1621 cacgacgagc acgccgacag cgagggctgc tgcaggtcga aagaacggga cgcgcatggc
     1681 agacatcctc tcccggatca ggggcgacgg ccgcgcaccg ctcccacgga ccaccggcgt
     1741 tgccgcctga ctcccggatc aggggcgccg gctgcgcacc gcctgctcga tccggccgag
     1801 cccctcgtcg agcagcgacc gagtcgtcgc gaagttgagc cgggcaaagc gcaggggctc
     1861 cccgccgaac ggcacccccg ggttgaccag cacgcgcgcc tgctcgacca gccacgccga
     1921 cggctcgacg tccagcccca gcccggtgaa gtcgagccac tgcagatagg tcccctcgtt
     1981 gggcacggtc gtcaccccgt cccagctggc caccgcctgc gcgaccagat cgcggtgctc
     2041 ggctagcgtt gccaccacct gggcgagcca ctcgccgccc tctcggtatg ccgcgatgct
     2101 cgcctccatc cccaaggtcg agaccccgac cgtctcccag tagggcattg ccttccacgc
     2161 ggcggtgtcg gcggcgttcg tcgtgatgac ctgggcgcat ttgaggcccg gcaggttcca
     2221 cgccttcgat gccgagagga ccgtgatgct gtggtcggcc gcctcgggtg aggcggcggc
     2281 atacgggaga tgtgggcggt cgaagaccaa cggagcgtgg atctcgtcgg agatgacccg
     2341 accgccgtgc cgcgcgacga cggccgcgag cccggcaagc tcggcggcgt cgaagacccg
     2401 accgagcggg ttgtgcggtg acgagatgag gacggtgcga ccaccggcgg cgaacgccgc
     2461 gtcgatggcg gccaggtcga aggtccaccg ggcgccgtcc caggacatcg ggacgtggac
     2521 ctgcgggcgg ccggtgaggc gcacgatgtc gaagaacggc atgtagacgg gcgtcgggat
     2581 gatcaccggg tcgtccggcc cgctgaagta gcggatcgcc aggtcgacgc cgtgcatgac
     2641 gtcgccgacg ctgtggaccc cggccggatc gatggcccag ccgtggtgct gtgcctgcca
     2701 ctcgacgagc gcgtcggcca actcggcatg gaccaccggc gaggggtagc cgaagctctc
     2761 ggccgcgacg gcgcgggtga gcgcggcggt gacctcacgg catacgggga agtccatctc
     2821 ggccacccac atgggcaaca gatccgcggg gtatgccgtc cacttgaggg tccgccgcgc
     2881 ccgcaactgg tcgggggtga gggcatgcag gtccagcggc acgggtcaac tcgcttcggg
     2941 ggtgtcggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggtagtcggc
     3061 cggggggacg ccgacgttgc gcgcgaagtg gtcgcgcagc agggctgccg tgccgaaccc
     3121 gctgcgcagg gcgacctgct cgatcggcag atcggtccgc tcgagcagcc ggcaggcgtg
     3181 gtgaagtcgt tggctggcaa tccatttcgc gggcgtcgtc ccggtctcgg cgaggaaccg
     3241 acgtgcgaag gtgcgttcgg acaggtgcgc gtgccgggcc agctcgcgga ccggcaggtc
     3301 ctcctcgagg tgctccatca tccaggtgag cagcggctcg aggctctcgg cggcgatcgt
     3361 ggggatcggc tgctcgacga actgccgctg accgccgtcg cgttgcgggg gaacgaccat
     3421 gcgccgggcg atccgggtcg cgacgtcggc gccgaactcg cggcggacca ggtgcaggca
     3481 ggcgtcgatg ctcgctgcgg tgcccgcgct ggtgaggatg gtgccgtcct cgacgaagag
     3541 gacggtcgcg tcgacggtgg ttgcgggata gcgctcggcg aattcggccg cgtggtacca
     3601 gtggcaggtc gccgtgcggc cgtcgagcag gcccgcctcg gcgaggacga agccgcccga
     3661 acatgtggcc aggatcgtgg cgccggcggc gtgggcctgg cgaacggcct caagcaccgg
     3721 ctcggagaag gtcttggcct gggtcgagga gacggcgaca acatcggcgc cgcgcagccc
     3781 gtcgaggccg aaggggacgg tgaggctcac cccgttgtcc atggggaggg gctcacccgg
     3841 ccgctcggcg cagacccgga actcgaacgg cggcaccccg tcagcggtcc ggtcgatgcc
     3901 gaaaacctcc gcgaggacgc cgaattcgaa cgcgagcggg cgttcggtga gcagcacggc
     3961 aacggtgcgc aacggcatac ccctgagtat gccgcgggtt tggcagaaaa gccacggtga
     4021 gggactgtcc tgccactatc ggcagtaatc agatgagggg atgattactg ccatggacct
     4081 catcttggca ctcgcgaccg tgatcggcgc gctcatcgtc cccatggaaa tcacccaccg
     4141 tcgcacgcgg agcctgcccc gcctgaccca actggagcgg tgcggcatcc gccagtggtg
     4201 gtgagccgac ccgcgtgtcg gccggcgcgc cagcccgcgc gtcggccgcc gcgttacagc
     4261 tcgtgctcgt cggcgtccgc gtcggccttg gtcgcgtgga ccgtgccgtc cgggtgctcc
     4321 ggcggcgaat agatcgaata cagcttgagc gggccgtgac cgcagttgat gacgttgtgc
     4381 caccggccgg cggggatgat gcaagcccaa tcctcgccca gcgcaatggt cttctcgatg
     4441 acctcgtcgt ccggcccgag cgtcaccatc gcggcaccct cctcgacgcg caggaattgg
     4501 tcgcggtcgt ggtgcacctc gagcccgatc tcctcgccgg gcgcgaggct catgacgacc
     4561 agttgcaggt gggctccggt cgccaccacc gtccggaagg tgtcgttgtc gagcgttgcg
     4621 cgctcgatgt tcccagtcca gccactcatg ggcctctcct tcgtctgctg actcgacgat
     4681 aaccccgggg ggcggcgccg cggcataccg gatgccatag ccttccccca tggcacgccc
     4741 gcacgcctcg gcggtagtcg aggacgcttg gaactcccaa cgggagcgcg tcctacgcaa
     4801 acgcgggtgg ggaacccggg tcatcagcca taccggctac ggctcgacgg acttcgtccg
     4861 ggtgttcgcc cgggtcgtgc tcggccgcgc gctgggcgac gacgacgagc cgcgtcagga
     4921 cgcgacctac ggcgggatgc gctcgcccta cctgcgccgg cgtggctggc gcgccttctt
     4981 cactgccccc gcgatgaacg tgccggtcga ggtgcacgtc ggccaccacg tggctcacgg
     5041 ccgcagtgac cgcagcggtc acatcgacct caccttccgc ggacacggta tgccgcccgg
     5101 ctggcacgag gtgaccgtca gcgcccaggg gtgcgacccc gtgcaggcga gcgtctacat
     5161 cgtggcgccg gacgcgcggt tcgggctgat cagcgatatc gacgacacgg tcatcacgac
     5221 gtcgctgccc cggccgatga tcgccgcgtg gaacaccttc ttccggcacg agaacgcccg
     5281 ccgggtggtg ccggggatgg ggccgctcta tcggacgatc atggccgagc atccgggcgc
     5341 gccgatcatc ttcgtgtcga cgggggcgtg gaacaccgcg ccgacgctga cccgcttcct
     5401 caagcgcaac ggtttcccca tcgggccgct gctgctcacc gactgggggc cgacgaacac
     5461 gggctggttc cgaagcggtc aggaccacaa gcgggcctgc ctggaccggc tggccaagga
     5521 cttccccaag atcaagtggc tgctcatcgg tgacgacggc cagcacgatc cgaagatcta
     5581 tcaggacttc ctcgaggcgc ggcccaagcg ggtggctgcc gtcgggatcc gtcagttgac
     5641 gccggccgag caggtgctct cgcacggttt gccggtgtcg accgaggagc tcaccggcaa
     5701 ggcgccgcgc ccgcgcacgg tggcgccgat cgtctatgcg ccggacggtt acgggctgtt
     5761 gcgcttgctg cgcgccgcgg gcctgctcgc gccgtcccct gccggtgagg cgggcctcgg
     5821 ggctgttgac ggctcggcca cgtccgaccc actcgactcc cactagagcc aggaggtcgc
     5881 cgtgccggag cttcccgaag tgcaggccct tgtcgacttc ctcgccgagc gtgcggtcgg
     5941 ccaggcggtg gtcgctgtcg agttgggctc gatctcggtg ctcaagacct acgacccgcc
     6001 gccatcggcg ctcgcgggcg ggcccatcga cggggtgcat cggcacggga agttcctcga
     6061 tgtcgacgtg gacggcatac atctggtgtt ccatctcgcg cgggccggct ggttgcggtg
     6121 gtcggaggcg atccccgcga cggtgatccg gccggggaag tcgccgatcg cgctgcgggt
     6181 gcggctgtcg gatgggtcgg gtttcgacct cacggaggcc gggacgaaga agtcgctcgc
     6241 ggcatacct
//
LOCUS       DAORPW010000129         6235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3367_length_6235_cov_641.608900, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000129 DAORPW010000000
VERSION     DAORPW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3367_length_6235_cov_641.608900"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLB86_09810"
     CDS             complement(<1..638)
                     /locus_tag="PLB86_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRC65471.1"
                     /translation="MTSPQLPRRSLLLGGLGVIGVGALAACTSSGAGHAGDAAHATNT
                     PHVGMDMGNGKTIGPVPSAGPSAGQKLVTATLTPKRTTLDLGGVTVDTWAYGDSVPGQ
                     AIRATAGDFLRVTVDNQLPADTTVHWHGIRLRNAADGVPGVTQDAIRPGARYVYEFTA
                     PDPGTFFFHPHVGLQLDRGLYAPLILDDPAEPGGYDAEWVVVLDDWLDGVGTT"
     gene            complement(643..894)
                     /locus_tag="PLB86_09815"
     CDS             complement(643..894)
                     /locus_tag="PLB86_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRC65472.1"
                     /translation="MMGWNGMGSGWMWLVMLAVIVGFWAVVVVLLRAVLRDRARDRDR
                     DAVEPTLESPLVILDRRLVSGEIDIDEYVQLRRAITSGH"
     gene            1019..1723
                     /locus_tag="PLB86_09820"
     CDS             1019..1723
                     /locus_tag="PLB86_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC65473.1"
                     /translation="MTASAGRVLVVEDEKDLAAMVGSYLQRAGYAVDLAFTGPDGVEA
                     ARSAEPDVVVLDLGLPGLDGIEVCRQLRTFTDCYVLMLTARTDEIDKIVGLSVGADDY
                     ITKPFSPRELVARVQVVLRRPRRPVVSGDDAQPPRVFGDLKVDLDGRQVRVAGHPVEL
                     TRTEFDILALLASSPKVAFSRRRILDEVWDPGWVGDEHVVDVHMAHLRRKLGDDPAEA
                     AYITTVRGVGYRMVTS"
     gene            1743..>2797
                     /locus_tag="PLB86_09825"
     CDS             1743..>2797
                     /locus_tag="PLB86_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC65474.1"
                     /translation="MNLRAARAGWSLRTRLMLAQGIVVGAGILTAALVAAIVGPQLFH
                     EHLLQAGQEPNSPELMHSEEAYASANTIALGAALLIALTAGFVVTWQLSRRMTQPLRD
                     LATVATRLSNGDYSARAPRPSAGPELDRLADTLNDVAERLESTEDTRRRLLADLAHEM
                     RTPLAVIGAHLDGLEDGVTEWTPETARVLRDHTERLVRLSSDVAEVSRAEEGRLEERA
                     IVEVSVGDLVRAAVEGVRSEFAAKGVELVGAGGGAAQVTGATGVDVAAGLEVLVTVDR
                     QRILQLLTNLLTNALRHTPAGGTVRVDAQTSGEHVELTVTDTGDGISATHLPHVFERF
                     YRGDTARDREHGGSGIGL"
     assembly_gap    2798..2897
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3024..3416
                     /locus_tag="PLB86_09830"
     CDS             3024..3416
                     /locus_tag="PLB86_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRC65475.1"
                     /translation="MTESAQKQQSSGHRIFATSFASIYPHYVAKAVRKGHTAAEVDEV
                     IAWLTGYDEAGLAIVLANEVDLQTFFAQAPAWNPNAALIRGTICGYRVQEIEDPLMQK
                     IRYLDLLVDEVARGKKMSAIQRGSTPTA"
     gene            complement(3594..5009)
                     /locus_tag="PLB86_09835"
     CDS             complement(3594..5009)
                     /locus_tag="PLB86_09835"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRC65476.1"
                     /translation="MTTHLPRPTPTRRAVLSMLGLGVLGLAGCAAGSGTDSTATSTGS
                     SGTSSTGASSAGASAGRGTGIFAADVIHTVTAAAAASVFTGALATFKSSGTKEWIDVE
                     VTLDGTTFQHVGMRLKGNSSLRSVAAGTAPQLLPWLVKLDKFVKGQAHEGVSEFVVRS
                     NSSATALNEAVALDLLAASGLSAQRAAYIALSVNDSAPALRLIVENPGDEWVDRVFNA
                     DGKLYKAEATGDYTYRGTDPAAYDEVFDQEAGDKDLTPLMGFLQFVNDSSDADFVAGL
                     ADRLDTAAFARYLAFETLVDNFDDIDGPGNNSYLWWDADTNKMTVVAWDHNLAFGLRP
                     NGGGARGGGAVPGGVPNGAVPDGAVPGGAVPDGAVIDGAVPGGGAPPGGGIGGPQGGG
                     RGPNGKVNPLVTRFTAQAQWQQAVTAAKTELTTTLVTSGAGATALARWQALLSGDSRG
                     LVPAATITTEAAAITTKLSAT"
     gene            complement(5209..5646)
                     /locus_tag="PLB86_09840"
     CDS             complement(5209..5646)
                     /locus_tag="PLB86_09840"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRC65477.1"
                     /translation="MDPALLDLTAGACRRLIEERFPDDDHHGAAAMLLRDGTILTGTS
                     PNAINPSVEVCHEIEPYCAAYRLDQSVVASVCLHRQPGGRFVVLSPCGVCRERLAVHG
                     PAVLVAVADPNDPSVIVWKTLKDVLPDYWMTAFPDEVDPAWSA"
     gene            complement(5769..>6235)
                     /locus_tag="PLB86_09845"
     CDS             complement(5769..>6235)
                     /locus_tag="PLB86_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRC65478.1"
                     /translation="SQVVDFVTVSTAGLESSPHAAALAGLRANESRYFKNKYDHDFTV
                     TPADDAPEVVAWVATILQERNIAIAARPLEVSVADVDGIRWVHVFYESGLAINVLYGV
                     EAGAKRAVGFKLSEGMEVPEELSGFKFARQKSKLAGIMRGSFFVIKGEYDPT"
BASE COUNT          983 a         2117 c         2090 g          945 t
ORIGIN      
        1 gtggtgccga cgccatcgag ccaatcgtca agcacgacaa cccattccgc gtcatagcca
       61 cctggctcgg ccgggtcgtc gaggatgagc ggcgcataga gcccacggtc gagttgcagg
      121 cccacatgcg ggtggaagaa gaacgttccc gggtcggggg cggtgaactc atagacatag
      181 cgagcgcccg gccggatcgc gtcctgcgtc actcccggga cgccgtcggc cgcattgcgc
      241 agtcgtatgc cgtgccagtg caccgtggtg tccgcaggca attggttgtc gaccgtcact
      301 cgcaggaagt cgcccgccgt ggcccgaatc gcttgtcccg gaacgctatc gccgtatgcc
      361 caggtgtcga cggtgacccc gccgaggtcg agggtggtgc gcttgggggt gagggtagcc
      421 gtgaccaact tctgtcccgc gctcggacca gccgacggca ccggcccgat cgtcttgccg
      481 ttacccatgt ccatgccgac gtgcggcgtg ttggtggcgt gtgcggcgtc gccggcatgc
      541 ccggcgccgc tcgaggtgca ggcggcaagc gcaccgacgc caatcacgcc caggcccccg
      601 agcaggaggg aacgacgagg cagctggggg gaggtcatcg gctcagtgtc cactggtgat
      661 ggcccggcgc aactgaacgt attcgtcaat gtcgatctcg ccgctgacca accgccgatc
      721 gaggatcacc agcggcgatt cgagcgtggg ctccactgcg tcgcggtcac ggtcacgggc
      781 ccggtcgcgc agcaccgcgc gcagcaagac gacgacaacc gcccagaacc cgacgatgac
      841 ggccagcatc accagccaca tccagcccga gcccatccca ttccaaccca tcatgggttg
      901 atgctcgcgc ctgcgggtgt cggacgggcg ctgcgaaacc tgaagatctc gtcaagatct
      961 gtgcgcacgt tgccgcaacg cgggccccca gcacgccacg cccgcgatga tgggtggcat
     1021 gacagcgtcg gccggccggg tgctcgtcgt cgaggatgag aaggacctcg cggcgatggt
     1081 cggctcctac ctccaacgcg ccgggtatgc cgtggacctc gccttcaccg gcccggacgg
     1141 tgtcgaggcg gcccgcagcg ccgagccgga cgtcgtcgtc ctcgacctgg gcctgcctgg
     1201 cctggacggc atcgaggtat gccgccaact gcgcaccttc accgactgct acgtgctcat
     1261 gctgaccgcc cgcaccgacg agatcgacaa gatcgtcggg ctgtcggtgg gcgccgacga
     1321 ctacatcacc aagccgttca gcccccgcga gttggtggct cgcgtccagg tggtcctgcg
     1381 tcgaccccgt cggccggtcg tgtccggcga cgacgcccag ccccccaggg ttttcggcga
     1441 tctcaaggtt gacctcgacg gtcgacaggt gcgggtcgcc ggccatcccg tcgagttgac
     1501 ccgcaccgag ttcgacatcc tcgccctgct cgccagctct ccgaaggtgg ccttcagccg
     1561 ccgacggatc ctcgacgagg tctgggatcc gggctgggtc ggcgacgagc acgtggtcga
     1621 cgtccacatg gcccatctgc gacgcaagtt gggagacgat ccggccgagg cggcatacat
     1681 caccacggtc cgaggcgtcg gctatcggat ggtgacgtcg tgaaccctgc gcgggtgacg
     1741 cggtgaacct ccgtgccgcg cgcgcgggct ggtcgctgcg cacgcggctc atgctggctc
     1801 agggcatcgt cgtcggcgcg ggcattctca ccgctgcgct cgtggcggcg atcgtcgggc
     1861 cgcagttgtt ccacgagcac ctgttgcagg cgggtcagga gcccaactcc cccgagctca
     1921 tgcactcgga ggaggcctac gcgagcgcca acaccatcgc gctcggcgcc gcactgctca
     1981 tcgcgttgac ggcgggattc gtcgtcacct ggcagctgtc ccgccgaatg acccaaccgt
     2041 tgcgcgacct ggcgacggtg gccacccggc tgtcgaacgg tgactactcg gcgcgggctc
     2101 cgcgtccgtc ggccgggccc gagctcgacc ggctggccga cacgctcaac gacgtcgccg
     2161 agcggttgga gtcgaccgag gacacccgac gccggctgtt ggccgacctc gcccacgaga
     2221 tgcgaacgcc gctcgccgtc atcggtgctc acctggacgg gctcgaggac ggcgtcaccg
     2281 agtggacgcc cgagaccgct cgcgtgctgc gcgatcacac cgagcgactg gtgcggctgt
     2341 ccagcgatgt cgccgaggtg tcgcgcgccg aggaaggccg cctcgaggag cgcgcgatcg
     2401 tcgaggtctc ggtgggcgac ctcgtgcgcg ctgccgtcga gggggtgcgg tcggagttcg
     2461 cggccaaagg ggtcgagttg gtgggtgccg gcggcggtgc cgcgcaggtg actggggcca
     2521 cgggagttga cgtcgcagcc ggactggagg tcctggtcac cgtggaccgc caacgcatcc
     2581 tccaactgct gacgaacctg ctcaccaacg cgctccggca cacgcccgcc ggtgggaccg
     2641 tccgggtcga cgcgcagaca tcgggcgagc acgtcgagct cacggtcacg gacaccggcg
     2701 acggcatctc cgccacgcac ctcccccacg tcttcgagcg cttctatcgc ggcgacaccg
     2761 cgcgcgaccg cgagcacggc ggcagcggca tcgggctnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnngca tcgtcggctt cgtgggcatc gtcggcacct ccggtcggct
     2941 ggctctcgac gaaagggcca gcgtagttgg ctcggcgggg cgatggtcgg ttagcgttgc
     3001 gccccaacga aagggggtgc cgaatgactg agtcggcgca gaagcagcag tcgagcgggc
     3061 accgaatctt cgcgaccagc ttcgccagca tctatccgca ctacgtggcc aaggccgtgc
     3121 gcaagggcca caccgccgct gaggtggacg aggtcatcgc ctggctcacc gggtatgacg
     3181 aggcgggcct ggcgattgtg ttggccaacg aggtcgacct gcagaccttc ttcgcgcagg
     3241 caccggcgtg gaaccccaac gctgccctca tccgcgggac gatctgcggc tatcgggtcc
     3301 aggagatcga ggacccgttg atgcagaaga tccggtatct cgacctgttg gtcgacgaag
     3361 tcgcccgggg caagaagatg tcggctatcc agcgcggcag cacccccacg gcctgaccct
     3421 ttcagccccc ggcggacggg cggccggtca gggccaacag gtgcgtctgc atgggtttcg
     3481 cgggcgggaa gccgcggatg aagccgcgac tagtggcgtg atcacgcatg cagcggcggc
     3541 ctcatgggcg gcttcatcac cgcaggcacc gtttgagcac cgaccatgca gcgtcaggtc
     3601 gcgctgagtt tcgtggtgat cgccgcggcc tcggtggtga tcgtcgccgc cgggaccagt
     3661 cctcgagagt cgcccgacag cagggcttgc caccgtgcca aggccgtggc gccggcaccg
     3721 gaggtcacca acgtggtggt gagttcggtc ttcgctgcgg tgactgcctg ctgccattgc
     3781 gcctgagcag tgaaccgggt gaccagcggg ttcaccttgc cgttcgggcc tcggccaccg
     3841 ccctgcggac cgccgatccc tccacctgga ggtgcgccac cgccgggcac tgcaccatca
     3901 atcacggcgc cgtcaggcac ggcaccacca ggcacggcac catcgggcac ggcaccattg
     3961 ggcacaccgc caggcacggc gccaccgccc cgcgcaccgc cgccgttcgg ccggagcccg
     4021 aacgccagat tgtggtccca ggccacgacg gtcatcttgt tcgtatcggc gtcccaccac
     4081 agatacgagt tgttgcccgg cccgtcgatg tcgtcgaagt tgtcgaccag cgtctcgaac
     4141 gccaggtacc tcgcgaaggc cgccgtgtcc aaccgatcag ccagacccgc gacgaagtcg
     4201 gcgtcgctgg agtcgttgac gaactgcagg aaccccatca gcggggtcag gtccttgtcg
     4261 ccggcctcct ggtcgaacac ctcgtcgtat gccgcggggt cggttccccg gtaggtgtag
     4321 tcgccggtcg cctcagcctt gtagagcttg ccgtcggcgt tgaacacccg gtccacccac
     4381 tcgtcaccgg ggttctccac aatcagccgt agcgcgggcg ccgagtcgtt gacgctcaag
     4441 gcgatgtatg ccgcgcgctg agccgacagt ccactggcgg cgagcaggtc aagcgccacc
     4501 gcttcgttga gtgccgtcgc ggaactgttg gaccggacga cgaactcgct caccccctcg
     4561 tgagcctgac ccttgacgaa cttgtcgagc ttgaccagcc acggcagcag ttgcggtgcg
     4621 gtgcccgcgg cgaccgagcg cagcgaggag ttgcccttca atcgcatccc cacgtgctgg
     4681 aacgtcgtgc cgtccagggt cacctcgaca tcgatccact ccttggtgcc gctgctcttg
     4741 aaggtcgcca acgcccccgt gaagacggac gccgcggccg cagccgtcac cgtgtggatg
     4801 acgtcggccg cgaagatccc cgtgccgcgc cctgccgagg cacccgccga cgacgctccg
     4861 gtcgatgagg tgccggacga accggtcgag gtcgcggtcg aatccgtgcc ggagccggcg
     4921 gcacacccgg ccaggccgag gacgccaagt ccgagcatcg agagcacagc ccgtcgggtc
     4981 ggggtgggcc ggggcaggtg cgttgtcatc gcccaaagac accctgggag cctgtgaacc
     5041 ccctgtgcgc agagcctccg tttcacgtga agcaggcgaa cggcatacag acgaatgcgg
     5101 cgacaaccgg gcgagaagcc gcccttcaat cgcgagctgc ccgagttgtc gaagcagggc
     5161 catgggccag cacaagcgcg gcggggcgcg gggggcacta gcgccggctc aggcgctcca
     5221 cgcggggtcg acctcgtcgg ggaacgctgt catccagtag tccggcagca cgtccttgag
     5281 cgttttccac acgatgaccg acggatcgtt cggatccgcg accgcgacca gaaccgccgg
     5341 gccgtgcacc gccaggcgct cacggcatac cccgcaggga ctgagcacca cgaacctgcc
     5401 cccgggctgg cggtgcaagc agaccgacgc gaccacagat tgatcgaggc ggtatgccgc
     5461 gcagtagggc tcgatctcgt ggcagacctc gacggacggg ttgatggcgt tcggggaggt
     5521 tcccgtgagg atcgttccgt cgcggagcag catggcggct gcaccgtggt ggtcgtcgtc
     5581 cgggaatcgc tcctcgatca accgtcggca ggcgccggct gtcaggtcaa ggagtgcggg
     5641 gtccatgccg tgaccatagg gcgcgtcgat gcggctgcgg gcgggaagcc gctcatgaag
     5701 ccgcgaccgg tggcgtgatc acgcacgcta cggcgacttc atgggcgact tcatcccctg
     5761 ccccgcgctc aggtggggtc gtactcacct ttgatgacga agaaggagcc gcgcatgatg
     5821 ccggcgagct tggacttctg ccgggcgaac ttgaaccccg acaactcctc gggcacctcc
     5881 atcccctcgg acagcttgaa accgaccgcc cgcttggccc ccgcctcgac gccgtagagc
     5941 acgttgatcg ccaggcccga ctcgtagaac acgtgcaccc agcggatgcc gtcgacgtcg
     6001 gcgaccgaca cctccaacgg ccgcgcggcg atcgcgatgt tgcgctcctg caggatcgtg
     6061 gccacccacg cgacgacctc gggggcatcg tcggccggcg taacggtgaa gtcgtggtcg
     6121 tacttgttct tgaagtagcg cgactcgttc gcccgcaggc ccgcgagcgc ggcggcgtgc
     6181 ggcgaggact ccaggcctgc cgtggacacg gtgacaaagt cgacgacttg gctca
//
LOCUS       DAORPW010000130         6146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3453_length_6146_cov_913.095879, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000130 DAORPW010000000
VERSION     DAORPW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3453_length_6146_cov_913.095879"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3467
                     /gene="smc"
                     /locus_tag="PLB86_09850"
     CDS             <1..3467
                     /gene="smc"
                     /locus_tag="PLB86_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRC65479.1"
                     /translation="AWVMGEQGAKSLRGGKMEDVIFAGTAGRAPLGRAEVALTIDNTD
                     GALPIDYSEVTISRTMFRNGGSDYAINGTSCRLLDIQELLSDSGIGREMHVIVGQGQL
                     DAVLRATPEERRGFIEEAAGVLKHRKRKERALRKLETMEANLTRVQDLTTEIRRQLGP
                     LGRQAETARKAGVIQADVRDARLRLIADDLVQLTATLEQEVADETALIARRTEVETAL
                     ASLGARLTALEVEADETRPQLAAAQQRWFALTRLVERFTSTATLAGERVRLLAEDEAD
                     NAGPASGPAIPRDPDALRAQAAALRTEHEELLAEITAATAALTAAVTARTESERRYAA
                     EQQRLAALVRAAADRREGLAKLAGQVAAKASRVEAGEAEVGRLQAGVGQILARSLEAE
                     REFARLEASIAVDEEGEEGLDAAYEKANAELTALQAEQERLREQERTADRDRQSATAR
                     AEALALSLTRRDGAATLLGSDGNPREGVLGAVSSLIEVVDGHEAAIAAALGWAADAVA
                     VASIDAAVAGVAALRAAESGRAGMLVGATARPGASGVRAALPAGARWAADVVSAPDAI
                     APAVHQLLDGVALVADVAAARTLVAEGITAVTPDGDVYAPGFVVGGDRGAPSLLEMES
                     ALEAARAAAAAAGRLVEQAKFALAGVRDRIADVQERVEASLERLHESDARMSAVGEQL
                     GHLGSVVRGAKGEAERTERAIAAAVAALDADRAAHAELAQRLAAASEEVPVDAEPDPS
                     VRDALEAEASSARTQETEARLTLRTREERARAVAERAETLEAAAVAELAARERQAARR
                     ERRARESRVATAVRSGAAYAAARLRESAERAGEAARAGEAARAERDAERSRLRREMGG
                     FQDELRELTDSVHRDEVARAQQRLRIETLTGRAMEEYGVDAEVLVEEFGPHQLVPVIP
                     DPNDPDTDPDALPEPTPFIREAQEKRLRSAERKLAALGRVNPLALEEYAALEERHAFL
                     TTQLEDLKASKRDLLDIVREIDERVEQAFVSAFHDTAAQFEGVFSRLFPGGEGRLLLT
                     DPDNMLTTGLEVEARPPGKKIKRLSLLSGGERSLVAVALLVAIFKARPSPFYIMDEVE
                     AALDDANLGRLLTLFEELRDSSQLIVITHQKKTMEIADALYGVTMRGDGVTTVVSQRM
                     RDVALATP"
     gene            3595..3831
                     /locus_tag="PLB86_09855"
     CDS             3595..3831
                     /locus_tag="PLB86_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65480.1"
                     /translation="MAALVLTMVVCLLLGLAVVLAVAVPARREGKDLLTPRGEEVVAR
                     VRERTEHVATATKERTEEVLATAKDKVGEVLPSR"
     gene            complement(3856..4548)
                     /locus_tag="PLB86_09860"
     CDS             complement(3856..4548)
                     /locus_tag="PLB86_09860"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC65481.1"
                     /translation="MTRPTVDSDLRPASQTLDRGLRVLEHITESLTPLTVAEVARDVG
                     MHRSITYRMLRTLEDHGLIARDELGRFQAGAGLAVLAGRFTPALRAVAGRHLLKLAVA
                     TEKTAFLVVRHGDEAVTIEVVEPPAAAAHVSYRPGLRHPITTGAPGIALLAGEPPHAD
                     ERPAVTQARSLGWATSTSEVIEGFRAVAAPVIDASGVCRAAIAVVFAGSGNVSGVADR
                     VMAHALALGLDL"
     gene            4742..5920
                     /locus_tag="PLB86_09865"
     CDS             4742..5920
                     /locus_tag="PLB86_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC65482.1"
                     /translation="MQRSMRWLSMGLAIGLAGSLAACSESKSTGAATGTDAGGEIKVG
                     VVLDITGAGASLGVPERQTVELLAEQVNAAGGINGRKLKLIIEDNQSTEDGAAKVTSK
                     LLNTEKVDILLGASRTGPSLAMRPLAEKAEKPMISFAANAKIVDGSKWVFKTAQNDRV
                     VLEKMVDDMKAKNITKVAVARDASGFGEGIPEMLTEIGKSAGITVTTVQKFAPDATDF
                     TSQMLGLRDSNPQAVLIWGIPPAAGLAQKAYKQLALNVPVYQSHGIGNQAFLDTAGDS
                     ANGLLAPMGRMLVADQLKADDPQKAVVTKFIADFKAKYNANPSTFAGHAYDGFMIAVE
                     SLKKAGTDPVKLRDAIEATSKWPGISGVFTMTPTDHSGLTKDALMIVTVDNKAWKVAG
                     "
     gene            5927..>6146
                     /locus_tag="PLB86_09870"
     CDS             5927..>6146
                     /locus_tag="PLB86_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65483.1"
                     /translation="MTDLLQLVFSGLAQGSVYGLVALGFVAIFAVREIVNLVQGEYAA
                     LAGLAAISAVGAGLPLLVAIVCVIPLVVG"
BASE COUNT          971 a         2166 c         2139 g          870 t
ORIGIN      
        1 tcgcctgggt catgggggag cagggcgcca agagcctgcg cggcggcaag atggaagacg
       61 tcatcttcgc cggcaccgcc gggcgggcac cgctgggccg cgccgaggtc gccctcacca
      121 tcgacaacac cgacggcgcg ctgccgatcg actactccga ggtgacgatc agccggacga
      181 tgttccgcaa cggcgggtcc gactacgcca tcaacggcac gtcctgccgg ctgctggaca
      241 tccaggagtt gctctccgac agcggcatcg gccgagagat gcacgtcatc gtcggccagg
      301 gccaactcga cgcggtcctg cgggcgaccc ccgaggagcg ccgcggtttc atcgaggagg
      361 ccgccggggt cctcaagcac cgcaagcgca aggaacgcgc gctgcgcaag ctcgagacga
      421 tggaggccaa cctcacccgg gtccaggacc tcacgaccga gatccgccgc cagctcggcc
      481 cgctgggccg ccaggccgag accgctcgca aggccggcgt catccaggcc gacgtgcggg
      541 acgcgcggct tcggctcatc gccgacgacc tggtccaact caccgcgacc ctcgagcagg
      601 aggtcgccga cgagacggca ctcatcgccc ggcgcaccga ggtcgagacc gcgctggcct
      661 cgctcggagc ccgcctcacc gcgctcgagg tcgaggccga cgagacccgc ccccaactgg
      721 ccgccgctca gcaacgctgg ttcgccctca cccggctcgt cgaacggttc acgtcgaccg
      781 ccacgctggc aggggagcgg gtgcgcctgc ttgccgagga cgaagccgac aacgccggcc
      841 ctgcgtccgg gcctgccatc cctcgcgacc ccgacgcgct gcgcgcccag gcggccgccc
      901 tgcgcactga gcacgaggag ctcctcgccg agatcaccgc cgcgacagca gccctcacgg
      961 ccgccgtcac cgcgcgcacc gagtccgaac gccggtatgc cgctgagcag caacgcctcg
     1021 cggccctcgt ccgcgcggct gcagaccgcc gcgaaggcct ggccaaactc gccggtcagg
     1081 tcgcggcgaa ggcgtcccgg gtcgaagcgg gcgaggccga ggtcgggcgc ctgcaggccg
     1141 gcgtcggcca gatcctcgct cgatcactcg aggccgagcg cgagttcgcc cggctcgagg
     1201 cgtcgatcgc cgtcgacgag gagggcgagg agggtctcga cgcggcatac gagaaggcca
     1261 atgctgagct gacggcgttg caggccgagc aggagcggtt gcgcgagcag gaacgcaccg
     1321 ccgaccgtga ccggcagtcc gcgacggcgc gcgccgaggc gctggccctg tccctcaccc
     1381 gacgggacgg cgcggccacc ctcctcggct cagacggcaa cccgcgcgag ggcgtcctcg
     1441 gagcggtgtc gtcgctcatc gaggtcgtcg acggtcatga ggcagcgatc gccgccgccc
     1501 tggggtgggc ggccgatgcg gtcgccgtgg cgtcgatcga tgccgcagtg gcgggcgtgg
     1561 cggctttgcg cgcagccgag agcgggcggg ccgggatgct cgtcggtgcg accgcgcgac
     1621 ccggtgcgtc cggggtccgg gccgcgctgc cggccggggc gcggtgggcc gccgatgtcg
     1681 tcagcgcgcc ggatgccatt gcgccggccg tgcaccaatt gctcgacggg gtggccctgg
     1741 tcgccgatgt cgcagcggcc cgaacgctcg tggccgaagg gatcactgcg gtcactcccg
     1801 acggcgacgt ctatgcgccc ggcttcgtcg tcggtggtga ccgtggcgcg ccgagcctgt
     1861 tggagatgga gtccgcgttg gaggcggctc gcgccgctgc tgccgccgct ggacgcctgg
     1921 tcgagcaggc caagttcgcg ctggcgggcg tgcgggatcg catcgccgac gtgcaggagc
     1981 gggtcgaggc cagcctggag cggctgcacg agtccgatgc ccggatgagc gccgtcggcg
     2041 agcagttggg ccacctcggc tccgtcgtcc gcggcgccaa gggcgaggcc gagcgcaccg
     2101 aacgcgccat cgccgccgcg gtcgctgctc tcgacgccga ccgagccgcg cacgccgagt
     2161 tggcgcagcg actggctgcg gcgtcggagg aggtccctgt cgacgccgag cccgacccga
     2221 gcgtgcggga cgccctggag gccgaggcca gcagtgcccg cacccaggag accgaagccc
     2281 ggttgaccct gcgcacccgc gaagagcgcg cccgcgccgt tgctgagcgc gccgagaccc
     2341 tcgaagccgc agccgtcgct gagcttgccg cgcgtgagcg ccaggccgct cgtcgggagc
     2401 gtcgggccag ggagtcacgg gtggcgaccg cggtccggtc gggggcggca tacgctgcgg
     2461 cccggttgcg ggagagcgcc gaacgcgcgg gggaggcggc ccgcgcgggg gaggcggccc
     2521 gcgccgagcg cgacgccgaa cgctcccggc tgcgccgtga gatgggtggg ttccaggacg
     2581 aattgcgcga gctcaccgac tcggtgcacc gcgacgaggt cgcccgcgcc cagcaacggc
     2641 tgcggatcga gaccctcacg gggcgcgcga tggaggagta cggcgtcgac gccgaggtgc
     2701 tcgtcgagga gttcggcccg caccaactcg tcccggtcat ccccgacccc aacgaccccg
     2761 acaccgaccc cgacgcgctg cccgagccga cccccttcat ccgggaggcc caggagaagc
     2821 ggctgcggtc ggccgagcgc aagctcgccg ctctcgggcg ggtcaacccc ctcgcgctgg
     2881 aggagtatgc cgccctcgaa gagcggcacg cgttcctcac cacccaactc gaagacctca
     2941 aggcctccaa acgcgacctg ttggacatcg tccgggagat cgacgagcgg gtcgagcagg
     3001 ccttcgtgtc ggcgttccac gacacggccg cgcagttcga gggggtcttc tcgcggttgt
     3061 tccccggagg cgaggggcgg ctgctgctca ccgaccccga caacatgctc acgaccggtc
     3121 tcgaggtcga agcgcgcccg cccggcaaga agatcaagcg gttgtcgttg ctgtccggtg
     3181 gggagcggtc actggtggcg gtggccctgc tcgtggcgat cttcaaggcc cggccgagcc
     3241 cgttctacat catggacgag gtcgaggcgg ccctcgacga tgccaacctt gggcgactgc
     3301 tgacgctttt cgaggagttg cgcgactcca gccaactcat tgttattacc caccagaaga
     3361 agacgatgga gatcgccgac gccctctatg gcgtgaccat gcgcggggac ggcgtgacca
     3421 ccgtggtgag tcagcggatg cgcgacgtcg cgctcgcgac cccctgatct tgacagccgc
     3481 atcccgaatc gtgagatcgg gggcgcccgg cccgttcagg tatgccgtga tccatgtcac
     3541 gtcataggcg cgccgaggcc ccgcttcggc cggtgatttg ggacacttgg tcccatggct
     3601 gcactcgtcc tcacgatggt tgtgtgcctc ctactcgggt tggctgtggt tcttgctgtc
     3661 gctgttccgg cccgtcggga gggaaaggac ctgctcaccc cccggggtga ggaggtcgtt
     3721 gcgcgcgttc gcgagcgcac tgagcacgtc gccacggcga ccaaggaacg cacggaagag
     3781 gtcctggcca cggccaagga caaggtcggc gaggtcctgc ccagccgctg acctgtcagc
     3841 gatcgcgctg ggccttcaga ggtccaggcc gagcgccagg gcgtgtgcca tcacgcgatc
     3901 ggccacaccc gacacgttgc cgcttccggc gaagacaacg gcgatggccg cacggcatac
     3961 ccctgaggcg tcgatgaccg gtgccgcgac cgcgcggaag ccttcgatca cctcgctggt
     4021 gcttgttgcc cagcccagac tccgtgcctg cgtgactgcg ggccgctcgt cggcgtgcgg
     4081 gggttcgccc gccaagaggg cgattccggg cgcgccggtg gtgatcgggt gacgcaggcc
     4141 gggtcggtag ctcacgtggg ccgcggccgc cgggggctcg acgacctcga tggtgaccgc
     4201 ctcgtcgcca tgtcgcacga cgaggaacgc cgtcttctcg gtggccaccg cgagtttgag
     4261 caggtgccga ccggcgacgg cgcgcagggc tggggtgaac cgcccggcga ggaccgccag
     4321 cccggcaccg gcttggaagc ggccaagctc atcgcgcgcg atcagtccgt gatcctccaa
     4381 cgtccgcagc atgcggtagg tgatcgaacg gtgcatcccc acgtcgcgcg cgacctcggc
     4441 cacggtgagg ggagtgagtg actcggtgat gtgctccagc acccgcagcc cgcggtcaag
     4501 cgtctgggat gccggtcgca ggtctgaatc gaccgttggc cgcgtcacat ttcgcgtgcg
     4561 cggaacaccc gttgccatcg tcaccccggt cgtctagagt ttgaccattc ccatatcgaa
     4621 cagcgctgtc cgattataga atgcctagac ggcggtgcgg tagagctcgg gacacagcaa
     4681 cacagcagtt ttccgacaca cagctgacgg cggggattcc cgccctggtt caggaggcaa
     4741 catgcagcgc agcatgcgtt ggctttcgat gggtctggca atcgggttgg cgggttccct
     4801 cgccgcctgt tccgagagca agtccaccgg cgccgcgacc ggcaccgatg ccggcggcga
     4861 gatcaaggtc ggcgtcgtcc tcgacatcac tggcgcggga gccagcctcg gtgtcccgga
     4921 gcgccagacc gtcgaattgt tggctgagca ggtcaacgct gcgggcggga tcaacggccg
     4981 caaactcaag ctgatcatcg aggacaacca gtccaccgaa gacggcgccg ccaaggtcac
     5041 cagcaagctg ctcaacaccg agaaggtcga catcctcctc ggcgcctcgc ggaccgggcc
     5101 cagcctggcc atgcggccgc tggccgagaa ggccgagaag ccgatgatct ccttcgcggc
     5161 caacgccaag atcgtcgacg gctccaagtg ggtcttcaag accgcccaga acgaccgcgt
     5221 cgtcctggag aagatggtcg acgacatgaa ggccaagaac atcaccaagg tcgccgtcgc
     5281 tcgtgacgcc tccggcttcg gcgaaggcat ccccgagatg ctcaccgaga tcggcaagtc
     5341 cgccggcatc acggtgacga cggtgcagaa gttcgccccg gacgccaccg acttcacgtc
     5401 gcagatgctc ggcttgcgtg actcgaaccc gcaagccgtc ctcatctggg gcatcccgcc
     5461 ggcagccggc ctggcccaga aggcctacaa gcagctggcc ctcaacgtgc cggtctacca
     5521 gtcgcacggc atcggcaacc aggccttcct ggacaccgct ggtgacagcg ccaacggcct
     5581 gctcgccccc atgggccgca tgctcgtcgc cgaccagctc aaggccgacg acccgcagaa
     5641 ggccgtcgtc acgaagttca tcgccgactt caaggccaag tacaacgcca acccgtcgac
     5701 cttcgccggg cacgcctacg acggcttcat gatcgccgtc gagtccctca agaaggccgg
     5761 caccgatccc gtcaagttgc gtgacgccat cgaggcgacg agcaagtggc cgggcatcag
     5821 cggcgtcttc acgatgactc cgacggacca ctcgggtctg accaaggacg cgctcatgat
     5881 cgtcacggtc gacaacaagg cctggaaggt cgcaggctaa gcactcatga ccgaccttct
     5941 ccagctcgtc ttctcagggc tggcgcaggg atccgtctac gggctggtgg cactcggttt
     6001 cgtcgctatc ttcgccgtcc gcgagatcgt caacctggtc cagggcgagt atgccgccct
     6061 ggccgggttg gccgcgatca gtgcggtcgg agccgggttg cccctgctcg tagccatcgt
     6121 ctgcgtcatt cccctcgtcg tcggtg
//
LOCUS       DAORPW010000131         6146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3454_length_6146_cov_648.073223, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000131 DAORPW010000000
VERSION     DAORPW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3454_length_6146_cov_648.073223"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..2000)
                     /locus_tag="PLB86_09875"
     CDS             complement(129..2000)
                     /locus_tag="PLB86_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65484.1"
                     /translation="MSSKVHKVNPKDRAQLNQTPVTFARVAGLFRPYRSKLLVVSLII
                     VTSSVISLGQPLLLRLIIDDALPYGRTTLLELAVAGMILVSIITGVLGVLQAWLATSI
                     GQQVMHRMRADVFANLQSQSMDFFKKTRGGEVQSRITSDITGMQSVITSTATTIAANV
                     TTVVATAIAMAALSWRLSLLSMVIIPPAVWMTRRVAIIRQSVTRQRQEAMSDLLSQVE
                     EALTVSGAQLIKTLGIADARQRQFESASASLSELELKSKLAGKWRTAVMNVTFASVPA
                     LIYLAAGFEATSSGLTLGTLIAFATLQVNVFKPTLGLLNIGAEWIASLALLSRIFDYL
                     DLPVDVPAPRHGIQVPGDHLLGPVRFEHVSYRYPDSETNILDDIDITVAPGTSIAVVG
                     ETGSGKSTLAALLVRLADPTEGRVCIGGFDVRELDHRSLTALVGIVSQDPYLSHTTIR
                     HNLLQARPEATEDMIWNVLTAAQIAETVERLPEGLDTVVGARGHRFSGGERQRLVIAR
                     TLLRDPKILILDEATSALDNATEKEVQVALDRLVLGRTTITIAHRLSTVRRADRIYVL
                     SGGRITEAGSHTDLLALGGLYAALASASQTNNGLDSSMPTPSAPQGADEHRLSFSPN"
     gene            complement(2215..>6146)
                     /locus_tag="PLB86_09880"
     CDS             complement(2215..>6146)
                     /locus_tag="PLB86_09880"
                     /inference="COORDINATES: protein motif:HMM:NF009589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRC65485.1"
                     /translation="PLSLEDKFTVDEGIIHLTGVQALVRLPMVQRRLDLAAGLNTATF
                     ISGYPGSPLGGFDLELQRRRGLMDAHHVVHQMGVNEELGATAVMGSQLAMQLPGPRFD
                     GVLGLWYGKANGFDRALDALRQANLAGTVRTGGALALVGDDPTAKSSPTPGASEVAAA
                     AIMMPMLYPGDVQEVLDLGQHAVALSRSCGLWVAVKMSTTVADGSGSAVVAPGRVQPI
                     MVDIEENGRPYLHRPSTHMYGARVMEMEHSMVYGRMRAALAYARANDVNRITLRTDAD
                     RVGIISSGKTYFEMRQTLRELGLDDRELQRLGVRLLQLRMPYPLEGGIIREFAAGLEE
                     ILVLEDKRPFVELFVKDELYGLADRPLVLGKLDEDGKWLVPIHAELDTVSIARLVAER
                     LLKRAAPGELAVLEERLERITRPRSLAPLAMSRTPYFCSGCPHNSSVAGVPAGTTVGA
                     GTGCHVLAVFMRPDEVGDILGITAMGNEGAQWIGASPFCSLPHFIQNLGDGTYAHSGS
                     LAIRASVAAGVNITYKLLYNDHVAMTGAQPAIGISGVPDLVTTLLAEGVRQVIITTED
                     LRRYAGVRLPDGAAVWDRSRLGEAVSVLSAVEGVTVLVHDQECATEKRRRRKRGTLEE
                     PKTRILINEAVCEGCGDCGIKSNCLSVHPVQTELGRKTQIHQPSCNLDYSCVQGNCPS
                     FVKVIPGTAVRPRRGASATLGADDLPEPTLRVPADEFGMRIAGIGGTGVVTVAQTLAT
                     AALLDGRFVRGLDQTGLAQKGGPVISDLRITTSPMDQANKLTVADCDLFLACDVLVGA
                     QQQLLTVTDSERTVAVTSTAQVPTGQMVVDTQLAFPDVDGLVARIDASTRSDLNVALD
                     APGIAERLFGTDQVANILLVGAAYQIGALPLRAESIEAALTLNGVAVETNLQAFRRGR
                     QAVADRAALDELVGASRVPAGAADLSLSATPTSGSSGSRPGIVLPRELRAITDLVGAE
                     PGSELARMVAVRVPMLVDYQDAAYARRYAVRLEAVRAAEAQRTPGRSTLAEAAAFSLF
                     KLMAYKDEYEVARLHRDPAFQADLAERFGGDAAYSVMLLPPVLKTIGLKRKVGLSRPV
                     AKVAFGALAPMKRLRNTRFDPFGRDHVRVVERALIAEYDALLDEIIQRLSVENHAVAV
                     ELAALPDLVRGYDEVKMRNVDRYREQVTRLRVQLTDGRLIVGRLDVDGATEAHREAES
                     QAGGASARPAAPEHSGSSAAWAGADSERGASASDPSPRPKRRRRSMHDNLEHDAASEG
                     RIEPSTAALMEGAPPGGTSGRVQPPRRTRTGVHDEGTNEGDTGNLPG"
BASE COUNT         1032 a         2091 c         2046 g          977 t
ORIGIN      
        1 agcccgacgc gcaggcgttg gagtcctgtt ggaacctcgg cgccaccctc gccgcgtccc
       61 tcatgcccgg gtagctacac ggcccctcac acccggccac ggcgccgatc ctggcccgcc
      121 gagccgcgtc agttgggaga gaaggacagg cggtgctcgt ccgccccctg gggagccgac
      181 ggtgtcggca tcgacgagtc gaggccattg ttggtttggg acgctgatgc cagagcggcg
      241 tagaggccgc caagcgccag tagatccgtg tgtgagcccg cttcggtgat acgccccccg
      301 ctgaggacat agatccggtc ggctcgtcgg acggtgctga gtcggtgggc aatggtgatc
      361 gtcgtgcggc caagaacgag ccggtccaaa gcgacttgaa cttccttctc cgtggcgttg
      421 tcgagagcgc tagtcgcttc atcaaggatg aggatcttgg ggtccctgag caaggtccgc
      481 gcgatgacca accgctggcg ctcgcctccg ctgaaacggt gaccgcgagc accgacgacg
      541 gtgtcgagac cctcaggtaa ccgctcaacg gtctccgcga tctgcgccgc ggtaagaacg
      601 ttccagatca tgtcctcggt cgcctcaggc cgcgcctgca ggaggttgtg gcgaatcgtc
      661 gtgtgggaca gatagggatc ttgagaaacg atacccacca acgcggtgag cgaccgatga
      721 tcgagttcgc ggacatcgaa cccgccgatg cagaccctcc cttccgtcgg gtcggcaagg
      781 cggacaagga gcgccgcaag ggtggacttg cccgaacccg tctcacccac gaccgcaata
      841 gacgttccag gcgcgacggt gatgtcgatg tcgtccagga tgttggtctc ggagtcggga
      901 tacctatagc tgacgtgctc aaagcgcacc gggccgagga ggtggtctcc cgggacctga
      961 atcccgtggc gaggagctgg cacgtcgacc ggcaggtcca ggtagtcaaa gatcctgctc
     1021 agcaatgcga gagaggcgat ccactccgcg ccgatgttca agagcccaag tgtgggcttg
     1081 aagacgttca cctggagcgt ggcgaaggcg atgagcgtgc cgagggtcag cccgctggag
     1141 gtggcctcga accccgcggc caagtagatg agggcaggca ccgaggcaaa agtgacgttc
     1201 atgaccgcag tccgccactt ccctgcaagt tttgacttca actccaactc gctgaggctc
     1261 gccgacgccg actcgaactg ccgctgacgg gcgtctgcga ttcccaacgt cttgatgagt
     1321 tgtgccccgc tgacggtcaa ggcttcctca acctgggaca acagatctga catcgcctcc
     1381 tgtcgctgac gggtgaccga ctggcggatg atcgccaccc gccgggtcat ccatacggcc
     1441 ggcgggatga tcaccattga cagcagagac agccgccagc tcagcgcggc catggcgatc
     1501 gccgtcgcca caacggtcgt tacattcgcg gcaatcgtcg tcgcggtgga ggtgatcact
     1561 gattgcatcc cagtgatatc gctcgtgatg cgggactgca cctcgccgcc gcgggtcttc
     1621 ttgaagaagt ccatcgactg tgactgaaga ttggcaaaga catccgcgcg cattcggtgc
     1681 atcacctgtt gcccgatgga cgtcgccagc catgcctgca gcaccccgag aaccccggtg
     1741 atgatggaga cgaggatcat ccccgccacg gctagttcaa gtagcgtggt cctcccatac
     1801 ggaagcgcgt cgtcgatgat gagtcggagc agcaggggct gcccaaggga gatgacggac
     1861 gaggtcacaa tgatcagcga caccacgagt agtttgctcc gatatgggcg gaacagcccc
     1921 gccacacggg cgaacgtgac cggcgtttgg ttgagttgcg ctcgatcttt tgggttgacc
     1981 ttgtggacct tggaactcac gtcgccctcc tgtgatacgc gtgacgaacc cgcgaacatc
     2041 tagtcgctca gaccccgatc ggcgacaacc atcatgacga gttcccccgt ttggtgtcgt
     2101 ggagtctcac ccgcggcgcc agcaaccaat gtagcccccg cctacgtgtc tcctgagtgg
     2161 cgttgaggta aacacgaggt gtccgccagg ttcgggacgg ccggaccgga gcggtcagcc
     2221 ggggaggttg ccggtgtctc cctcgttggt gccctcgtcg tgaacgccgg tccgcgtccg
     2281 ccgcggcggc tggacccgtc cgctcgtccc gccgggaggg gcgccttcca tgagcgcggc
     2341 cgtgctgggc tcgatccggc cctcgctggc tgcgtcgtgt tcgaggttgt cgtgcatcga
     2401 gcgccgccgc cgcttgggcc ggggcgaggg gtccgacgca ctggcgccgc gttcggaatc
     2461 ggcaccggcc cacgccgcgc tcgaccctga gtgctcaggc gccgcaggcc gagccgaggc
     2521 cccgcccgct tggctctcgg cctcgcggtg ggcctccgtt gcaccgtcga cgtcgagccg
     2581 cccgacgatc aagcggccgt cggtgagctg gacccgcaac cgggtcacct gctcgcgata
     2641 gcggtcgacg ttgcgcatct tgacctcgtc gtaccctcgc accaggtccg gcagcgccgc
     2701 caattcgact gccacagcat gattttcgac actcagccgc tgaatgatct cgtccagcag
     2761 cgcgtcatac tcggcgatga gggcgcgctc aaccacccgt acatggtcac gcccgaacgg
     2821 gtcgaaccgg gtgttccgca accgcttcat cggcgccagc gccccgaagg caaccttcgc
     2881 caccggccgc gacaacccca ccttgcgctt gaggccgatc gtcttgagca cgggcggcag
     2941 gagcatgacg gagtatgccg cgtcgccccc gaaccgctcg gccagatcgg cctggaaggc
     3001 cggatcccga tgcagccggg caacttcgta ttcgtccttg taggccatga gtttgaacag
     3061 cgagaacgct gcggcctcgg ccagcgtgct tcggcccggc gtgcgctggg cctcggccgc
     3121 gcggacggcc tccaggcgca cggcataccg gcgggcgtat gccgcgtcct ggtagtccac
     3181 gagcatggga acccgcaccg cgaccatccg agcgagctcc gaacccggct ctgcgccaac
     3241 gagatcggtg atcgcacgca gctcgcgcgg caggacgatc cccggccgtg accccgacga
     3301 ccctgaggtt ggcgtcgccg acagcgacag gtcggcagcg cccgcaggca cgcgcgaggc
     3361 gcctacaagc tcatcgagcg cagcccgatc ggcaacggcc tggcgcccgc gccggaaggc
     3421 ttgcaggttg gtctcgacgg ccacgccgtt gagggtgagg gccgcctcaa tcgactcggc
     3481 ccgcaagggc agcgccccga tctgatatgc cgcccccacc agcaggatgt tggcgacctg
     3541 gtcggtgccg aagagccgct cggcgatgcc cggggcgtcg agggccacgt tgaggtccga
     3601 ccgggtggag gcgtcgatcc gggccacgag cccgtcgacg tcggggaagg cgagttgggt
     3661 gtcgacgacc atctgcccgg tcggcacctg agccgtcgag gtcaccgcca ccgtgcgctc
     3721 ggagtcggtc accgtcagca actgctgctg agccccgacc aggacgtcac aggcgaggaa
     3781 gagatcgcag tcggcgacgg tgagcttgtt agcctgatcc atcgggctgg tcgtgatccg
     3841 caggtcggag atgaccggcc cgcccttctg ggccaacccg gtctggtcca gaccgcgcac
     3901 gaagcgcccg tcgagcaggg ccgcagtggc caacgtctgg gccacggtga cgacacccgt
     3961 gccgccgatg ccggcgatcc gcatcccgaa ctcgtcggcc ggcacccgca gggtcggctc
     4021 cggcaggtcg tcggcgccga gggttgccga ggcgccccgc cggggccgca ccgccgtgcc
     4081 cggaatgacc ttgacgaacg acggacagtt gccctggaca cacgagtagt cgaggttgca
     4141 cgacggttgg tggatctggg tcttgcggcc caactcggtc tggaccgggt gcaccgacag
     4201 acagttcgac ttgatgccac agtcgccgca gccttcacag accgcctcgt tgatgaggat
     4261 ccgggtcttg ggctcctcga gtgtcccgcg cttgcgccga cgacgcttct cggttgcgca
     4321 ctcctggtcg tgcaccagga cggtgactcc ttcgaccgcg ctgagaacgc tcacggcctc
     4381 ccccaaccgg gagcggtccc acaccgcagc gccgtcgggg agtctcacac cggcataccg
     4441 gcgcaggtcc tcggtcgtga tgatgacctg gcggaccccc tcggccagca gcgtggtcac
     4501 caggtcgggg acgcccgaga tgccgatggc cggctgcgcg ccggtcatcg ccacgtggtc
     4561 gttgtagagc agtttgtagg tgatgttgac cccggcggcg accgaggcgc gaatggccaa
     4621 agaccccgag tgggcgtagg tgccgtcgcc gaggttctgg atgaagtgcg gcaggctgca
     4681 gaagggcgac gcgccgatcc actgggcacc ctcgttgccc atcgcggtga taccgaggat
     4741 gtcgccgacc tcgtcggggc gcatgaagac ggcgagcacg tggcaccccg tcccggcgcc
     4801 gaccgtcgtc cccgcgggga ctcccgccac cgaggagttg tgggggcacc cggagcagaa
     4861 atagggggtc cgcgacatgg ccagcggcgc cagggagcgc gggcgggtga tccgctccag
     4921 ccgctcctcc aacaccgcga gctcaccggg agcggcccgc ttgagcagcc gttcggcgac
     4981 caaccgggcg atcgacaccg tgtctagctc ggcgtggatc gggacgagcc acttgccgtc
     5041 ctcgtcgagt ttgcccagca cgagcggccg gtcggccagc ccatagagct cgtccttgac
     5101 gaacaactcg acgaaggggc gcttgtcctc caggacgagg atctcctcca gcccggcggc
     5161 gaactcccgg atgatcccgc cctcgagcgg gtagggcatc cgcagctgca gcagccgcac
     5221 accgaggcgc tgcaactccc ggtcgtccag ccccaactcc cgcaaggtct ggcgcatctc
     5281 gaaataggtc ttgccggacg aaatgatccc cacccggtcg gcgtcggtgc gcagggtgat
     5341 ccggttgacg tcgttggccc gggcataggc cagggctgcc cgcatccggc catagaccat
     5401 ggagtgctcc atctccatga cccgggcgcc gtacatgtgg gtcgagggtc ggtgcagata
     5461 gggccgcccg ttctcctcga tgtccaccat gatcggctgg acgcgccccg gagccaccac
     5521 cgcagacccg gacccgtcgg cgaccgtggt cgacattttc accgccaccc acagaccaca
     5581 cgaccgcgag agggccacgg cgtgctgccc caggtcgagg acctcttgga catcgcccgg
     5641 atagagcatc ggcatcatga tcgctgccgc cgcgacctca ctggcccccg gcgtcggcga
     5701 cgacttggcg gtcgggtcgt ctccgaccag ggccaacgcg ccgcccgtac gcaccgtgcc
     5761 ggcgaggttg gcctggcgca gcgcatccag ggcccggtcg aacccgttgg ccttgccgta
     5821 ccagaggccc aacaccccgt cgaaacgcgg gcctggcaac tgcatcgcca gttggctgcc
     5881 catgacggcg gtcgccccga gctcctcgtt gactcccatc tgatggacga cgtgatgggc
     5941 gtccatcagc ccccggcgcc gctgcagttc caggtcaaaa ccgcccaacg gcgagcccgg
     6001 atagcccgag atgaaggtgg cggtgttgag accggccgcg aggtccagcc gtcgctgcac
     6061 catgggcaac cggacgaggg cctgcacccc cgtgaggtgg atgatcccct cgtcgacggt
     6121 gaacttgtcc tccagcgaca gcggtg
//
LOCUS       DAORPW010000132         6128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3474_length_6128_cov_649.241067, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000132 DAORPW010000000
VERSION     DAORPW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3474_length_6128_cov_649.241067"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="PLB86_09885"
     CDS             <1..1567
                     /locus_tag="PLB86_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC65486.1"
                     /translation="TSTRAGSVSMSYAASPADSAPSAPAPPGSTPQTSGPQMSGAERA
                     VFALGDVFGGGAAATLAVLYLFYLTDIVGLPPGLAGTTVVVSKVWDAINHPLMGIITD
                     RTRTRWGRRRPWIFAGALLLPPAMAGLWAPIGDWDSQSAKVWFVILAHLFWTTVASIV
                     AVPYGSLSTEITTDPDERSRVNVLRLGCATFSAVGCTLVMNALVNAFTEGHLSMTTLY
                     LSLFLGFGTFFAVPLLFVARFTHERAPVAEHPDAFNLKVVISPFRIASFRRLTAMYLC
                     PSITIDIVTSVILYYALYAVPGVRTVLFAGIFAVVTLVMLPVLLRLVDRVDKNVIYYT
                     GIPLSILAMVAIAFYPSGTIVWPVYVLAGIFAIGLAAAQVMIWVMFPDVVDDGEIAQG
                     ARNAGSFSGVMVLVRTMASALATFAVGWVLEFTGYQHARHGEARLAQPESALWGIRLT
                     MLITVVVLMGLAWFLARGYTLNRARVLQLSVDLERARAARHTVAPEHAAHHGAHEPGE
                     HEAGEHEPDQPHI"
     gene            1691..3010
                     /locus_tag="PLB86_09890"
     CDS             1691..3010
                     /locus_tag="PLB86_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HRC65487.1"
                     /translation="MTSPATVVVLGAAGRDFHNVNVVFRDDPAYRVVAFTATQIPDID
                     DRRYPPELAGPLYPDGIPIVPETDVVELIEREGIDLAVFAYSDVPHSRVMHLASQCLA
                     AGADFTMLSGRHTMLTSSKPVVAVTAVRTGVGKSQTTRYLAKLLREKGLRVVAVRHPM
                     PYGDLAAQAVQRFAAYADLDRYECTIEEREEYEPHIDAGCVVYAGVDYERILRQAETE
                     ADVILWDGGNNDLPFYRPDLHVCLVDPLRPGDERRYHPGEANVRMADLVIVNKCDAAR
                     PEDIDAVEASVREMNPLAQIHRCDSPVTVEGGAAVEGKDVIVVEDGPTLTHGSMTFGA
                     GVVGASAARVSRIVDPSPYAVGSLAATYAKYPNACGVLPAMGYSSEQIRDLEATIEAT
                     PADAIVSGTPIDLTRVLTVSKPIVRASYELREREPGRLRAALDAVLG"
     gene            3115..3315
                     /locus_tag="PLB86_09895"
     CDS             3115..3315
                     /locus_tag="PLB86_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HRC65488.1"
                     /translation="MAGKFEVYQDSAGKFRFRLKATNGQVIAVGQAYETKKSCLNGID
                     SIRKNAPDAKLDDQTAAPAAKA"
     gene            3341..>3762
                     /locus_tag="PLB86_09900"
     CDS             3341..>3762
                     /locus_tag="PLB86_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC65489.1"
                     /translation="MNPAHLQVDRVHAWLSQEAYWALGRTRETVEKAIAGSRTMGVYR
                     DRGDGMPGEQVAFARAVTDGATFAWVCDVFVDSDVRGLGVGNAMVGQLVTALEDLGIG
                     RVLLATRDAHEVYARLGFEPLANPTFWMERDRRPQRFVP"
     assembly_gap    3763..3862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3937..>4937
                     /gene="argF"
                     /locus_tag="PLB86_09905"
     CDS             3937..>4937
                     /gene="argF"
                     /locus_tag="PLB86_09905"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRC65490.1"
                     /translation="MAVDLTNRHFVKEIDFTPTEWMGLVGLTRSLKAARREGREERML
                     VGKTIALIFEKTSTRTRCAFEVAAYHLGAQLTYLDPHGSQIGHKESFADTGRVLGRLY
                     DAIEFRGSRHEDCETLARYAGVPVYNGLTDMWHPTQALCDIVTMVEHSPKPAAEISYA
                     YVGDARYNMGNSLLVAGAMSGMDVRIVAPRQLWPSVEIVGHARRLAETTGARIALTED
                     LDEGVAGVDFVHTDVWVSMGEGDAVWGERIELLTPYQVTSTVMASTGNPTTKFMHCLP
                     SFHNRETAVGEKIFAEFGLEAMEVTDEVFESPASIVFDQSENRMHTIKAVLAATLVG"
     assembly_gap    4938..5037
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5132..5617
                     /locus_tag="PLB86_09910"
     CDS             5132..5617
                     /locus_tag="PLB86_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC65491.1"
                     /translation="MARFNWEALPTRWQRADLDDPLNVVILLRQALLGAERAVHEVTD
                     GLLREAVGPGDEIALLHILRRVRGRETVVAQLADFLRCTHPVISRRVKRLEDAGLVLR
                     SGSWRDARSSSLTLTAAGSELVDDLNSALGELAQLWGDEIETDRWVVLMKALDALADA
                     A"
     gene            5619..6059
                     /locus_tag="PLB86_09915"
     CDS             5619..6059
                     /locus_tag="PLB86_09915"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HRC65492.1"
                     /translation="MTSSTAVVAAAIVDSLDCPSRLLAARRTYPVEVAGRWELPGGKV
                     EPGETATDALHRELAEELGIHLTLGGQIVGPLAGGAWPLTPAHHLLVWLAQVREGGIP
                     TPLQDHDEVRWLTADTLYAVPWLPGDLPVVEALAPLMVGEPLNT"
BASE COUNT          915 a         2087 c         1945 g          981 t
ORIGIN      
        1 cacgtccacc cgagcaggga gcgtgtccat gtcgtatgcc gcgtcgccgg ccgacagcgc
       61 gccctccgca cccgcaccgc ccgggtccac cccccagacg tccgggccgc agatgtccgg
      121 ggccgagcgg gcggtcttcg ccctcggcga cgtcttcggg ggaggggccg cggcgactct
      181 cgcggtcctc tacctgttct acctcaccga catcgtcggg ttgccccccg gcctggccgg
      241 cacgaccgtc gtcgtctcca aggtctggga cgcgatcaac cacccgctca tgggcatcat
      301 caccgaccgc acccgcaccc gctggggccg gcgccgcccg tggatcttcg ccggggcgtt
      361 gttgctgcca ccggccatgg ccggcctctg ggcgccgatc ggcgattggg acagccagag
      421 cgccaaggtg tggttcgtca tcctcgccca cctgttctgg accaccgtgg cgagcatcgt
      481 cgccgttccc tacggatcgc tgtcgaccga gatcaccacc gacccggacg agcgcagccg
      541 ggtcaacgtc ctgcgccttg gctgcgccac cttctcggcc gtcggctgca ccctcgtgat
      601 gaacgccctc gtcaacgcgt tcaccgaggg ccacctgtcg atgacgacgc tctacctgtc
      661 gttgttcctc ggcttcggca ccttcttcgc ggtgccactg ctgttcgtcg ccaggttcac
      721 ccacgaacgg gcaccggtcg cggagcaccc cgacgcgttc aatctcaagg tcgtcatctc
      781 accgttccgg atcgcctcct tccggcggct caccgcgatg tacctctgcc cgtcgatcac
      841 catcgacatc gtcacctcgg tgatcctcta ctacgccctg tatgccgtgc cgggagttcg
      901 caccgtcctc ttcgccggga tcttcgccgt ggtgaccctc gtcatgctcc cggtgctcct
      961 gcgcctcgtg gaccgggtcg acaagaacgt catctactac accgggatcc cgctctcgat
     1021 cctcgcgatg gtggccatcg ccttctaccc atcgggcacc atcgtctggc cggtctacgt
     1081 cttggcaggg atcttcgcca tcggcttggc ggcagctcag gtgatgatct gggtgatgtt
     1141 ccccgacgtc gtcgacgacg gtgagatcgc tcaaggggcc cgcaacgccg gatcgttcag
     1201 cggggtgatg gtcctcgtcc gcaccatggc ctcggcgctc gcgaccttcg cggtcggttg
     1261 ggtgctggag ttcacgggct atcagcatgc ccggcacggc gaggcccggc tcgcccagcc
     1321 cgagagtgcg ctctggggga tccggttgac catgctcatc accgtggtcg tcctcatggg
     1381 cctggcctgg ttcctggctc ggggttacac cctcaaccgg gcccgggtgc tgcaactcag
     1441 cgtcgacctg gagcgggccc gggcggcacg gcataccgtc gctcccgaac atgccgcgca
     1501 ccacggtgcg catgagccgg gggaacacga ggccggcgag cacgagcctg accaacccca
     1561 catctgagga cgggccgaag gtccctgccc tcgcggcagc cgctcataca gtggattacg
     1621 gcgagtccca cggtcggggc gccacgaggg catcccggcg ccggtgttcg acccagccac
     1681 ggagcgcgtt atgacaagcc ctgccacggt agtcgtcctc ggcgcagcgg gtcgtgactt
     1741 ccacaacgtc aacgtcgtgt ttcgcgacga tccggcgtat cgcgtagtcg ccttcaccgc
     1801 cacccagatc cctgacatcg acgaccggcg ttatcccccg gagttggccg gcccgctcta
     1861 tcccgacggg atcccgatcg ttcccgagac cgacgtcgtc gagctcatcg agcgcgaagg
     1921 aatcgacctg gcggtcttcg cctactccga cgtcccgcac agccgggtca tgcatctggc
     1981 gtcccagtgc ctggccgcgg gtgccgactt caccatgctc agcggtcgtc acaccatgct
     2041 cacctccagc aagccggtcg tcgccgtgac cgcggtgcgc acgggggtcg gcaagagcca
     2101 gaccacccgc tatctggcga agttgttgcg cgagaagggt ttgcgtgtcg tcgccgttcg
     2161 ccatccgatg ccctatggcg acctggccgc gcaggccgtc cagcggttcg ccgcctatgc
     2221 cgacctcgac cgctacgagt gcacgatcga ggagcgcgag gagtacgaac cccacatcga
     2281 cgcgggctgc gtcgtctatg ccggcgttga ctacgagcgg atcctgcgcc aggccgagac
     2341 ggaggccgac gtcatcctct gggacggcgg caacaacgac ctcccgttct accggccgga
     2401 cctgcacgtc tgcctcgtcg acccactgcg ccctggtgac gagcgccgct accacccggg
     2461 cgaggccaac gtccgcatgg ccgacctggt catcgtcaac aagtgcgacg cggctcggcc
     2521 cgaggacatc gacgccgtcg aggcgtcggt ccgcgagatg aacccgttgg cccagatcca
     2581 ccggtgcgac agccccgtca ccgtcgaggg tggcgcggcc gtcgaaggca aggacgtcat
     2641 cgtcgtcgag gacgggccga ccctcacgca cggctcgatg accttcggcg ccggagtggt
     2701 cggggcgagc gcagcccgcg tctcgcgcat cgtcgacccc tccccgtatg ccgtcgggag
     2761 cctggccgcg acctacgcga agtaccccaa cgcgtgcggg gtcctgcccg caatgggcta
     2821 ctcgtccgag cagattcgcg accttgaggc caccatcgaa gcaacgccgg cggacgccat
     2881 cgtcagcggc acgcccatcg acctcacccg ggtcctcacc gtcagcaagc ccatcgtccg
     2941 ggccagttac gagttgcgtg agcgcgagcc tgggcgactg cgggcggcgt tggacgcggt
     3001 gttgggctga ccccaggccg cccgagtcgg cgtggcgtgt ccgatgggcg cgccggaacg
     3061 agtggcgcgt cctacgctgc gaatgtcaat gtcactcgtg agtgggaggt tcacatggca
     3121 ggcaagttcg aggtctacca ggacagtgcc gggaagttcc gcttccgtct caaggccacc
     3181 aacggtcagg tcatcgccgt gggtcaggcc tacgagacga agaagagctg cctcaacggc
     3241 atcgactcga tccgcaagaa cgccccggat gccaagctcg acgaccagac ggcggctccc
     3301 gctgccaagg cctgacgtcc tggacggctt cgtcttcagc atgaacccgg cccacctgca
     3361 ggtggaccgg gttcatgcgt ggttgtcgca agaggcctat tgggccctcg ggcgcacgcg
     3421 agagaccgtc gagaaggcga tcgcggggtc gcgcaccatg ggtgtctacc gcgaccgcgg
     3481 cgacggtatg ccgggtgagc aggtcgcgtt cgcccgcgcc gtcaccgacg gggccacctt
     3541 cgcgtgggtg tgcgacgtgt tcgtcgactc cgacgtgcga ggcctcgggg tgggcaacgc
     3601 gatggtgggc cagttggtga cggcgctgga ggacctgggg atcggacggg tcttgttggc
     3661 cacccgcgac gctcacgagg tctacgcccg gctcggtttc gagccgctgg ccaatccgac
     3721 gttctggatg gaacgcgacc gtcgaccgca gcggttcgtg ccnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nntgatccac cccgggtgat ccacctcgcg tgggcagcga
     3901 cgccgcggtg gcgagcggac ggcatacggt tggcgcatgg ctgtggacct caccaaccgg
     3961 cacttcgtca aggagatcga cttcaccccc accgagtgga tggggctcgt ggggctgact
     4021 cgcagcctca aagctgcccg ccgcgaaggt cgcgaggagc ggatgctcgt cggcaagacg
     4081 atcgcgttga tcttcgagaa gacctcgacc cggacccgat gtgccttcga ggtggcggcc
     4141 taccacctgg gtgcccagct cacctacctc gacccgcacg gcagccagat cgggcacaag
     4201 gagtcgttcg ccgacaccgg tcgggtgctc ggccggctct acgacgccat cgagttccga
     4261 gggtcacggc acgaggattg cgagaccctg gcgaggtatg ccggtgtgcc cgtctacaac
     4321 gggctaaccg acatgtggca cccgactcag gcgctgtgcg acatcgtcac catggtcgag
     4381 cactcgccca agccggccgc cgagatctcc tacgcctacg tcggggatgc ccgctacaac
     4441 atggggaact cgttgctcgt cgccggcgcc atgtcgggga tggacgtgcg gatcgtcgcc
     4501 ccccggcagt tgtggcccag cgtcgagatc gtcggacacg cccggcgact cgccgagacc
     4561 acgggtgccc ggatcgcgtt gaccgaggac ctcgacgaag gcgttgccgg agtcgacttc
     4621 gtgcacaccg acgtgtgggt gtcgatgggt gagggtgacg cggtgtgggg cgagcggatc
     4681 gaactcctca cgccttacca agtgacctcg accgtcatgg cgtccaccgg aaacccgacc
     4741 acgaagttca tgcactgcct gccctcgttc cacaaccgcg agacggcggt gggggagaag
     4801 atcttcgccg agttcggtct tgaggcgatg gaggtgaccg acgaggtgtt cgagtcgccg
     4861 gcgtcgatcg tcttcgacca gagcgagaac cggatgcaca ccatcaaggc ggtcctcgcc
     4921 gcgaccctgg tgggtcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggg
     5041 ggtatgtccc ctggctgtgg cagcgccgac gcctttcggc ccctgtggat agcgcccggc
     5101 gtccccagtg acagtgcgct tcgatagggg catggcgcga ttcaactggg aggccctgcc
     5161 cacccgctgg cagcgagcag acctcgacga cccgctcaac gttgtcatcc tgcttcgcca
     5221 ggcactgttg ggtgccgagc gcgcggttca cgaggtgact gacggcctcc tccgcgaggc
     5281 cgtcggccca ggcgacgaga tcgccctcct ccacatcctg cgccgcgtca ggggtcgaga
     5341 aaccgtcgtc gcacagttgg cggacttcct ccgctgcacc cacccggtca tcagccgacg
     5401 ggtcaaacgg ttggaggacg cggggctggt cctgcgatcc ggttcgtggc gagacgcccg
     5461 gtcgtcgtcc ctcacgctga cagccgccgg aagcgagctc gtcgacgact tgaacagcgc
     5521 gctcggggag ctggcgcagc tgtggggcga cgagatcgag accgacaggt gggtcgtact
     5581 aatgaaggcg ctggacgcct tggccgatgc ggcctgagat gaccagttcg acggcggtcg
     5641 tggccgcagc gatcgtggac tcactcgact gcccctcccg cctgctggcc gccaggcgca
     5701 cctacccggt cgaggtcgcc ggccgatggg agcttcccgg cggcaaagtc gagcccggtg
     5761 agaccgcgac cgacgccctg catcgtgaac tcgccgaaga gctcggcatc cacctcacgt
     5821 tgggcggcca aatcgtcggc ccactcgcag gcggcgcctg gccgctcacc cccgctcacc
     5881 acctgctcgt gtggctggcg caggtgcggg agggcggcat accgacgccg ttgcaggacc
     5941 acgacgaggt gcgctggctg accgccgaca ccctgtatgc cgtgccctgg ctccccggcg
     6001 atctccccgt tgtggaggcc ctggcccccc tgatggtcgg tgagcccctg aatacctgag
     6061 agactgatcc ttatgcccac gcagcaccgc ggcgacatca gaaacgtcgc catcgtcgcc
     6121 cacgtcga
//
LOCUS       DAORPW010000133         6112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3486_length_6112_cov_759.591217, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000133 DAORPW010000000
VERSION     DAORPW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3486_length_6112_cov_759.591217"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1961)
                     /locus_tag="PLB86_09920"
     CDS             complement(<1..1961)
                     /locus_tag="PLB86_09920"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRC65493.1"
                     /translation="MNFFSIYDHGFARVAACTVPVRLVDPAANVAATLAVARECHDEG
                     VAVAVFPELGLTGYSLDDLVLADPLLDAVEVALDELVRASAELLPVLVVGAPLRARGR
                     VYNCAVVVHRGRVLGVAPKSYLPTYREFYESRTIAAGDDVRGTIRVGEADVPFGPDLL
                     FRAVDVPDLVVHVEVCEDMWVPIPPSAEAALAGATVLVNVSGSPITVAKARDRAMLAE
                     SASSRCLAAYLYAAAGEGESSTDLSWDGQTMVWENGSLLAEGERFPSGPRRSVADVHL
                     ARLRQERMRQGTFDDNRRTHAARVGAFREVTFRLDPPRGDLGLRRAVDRYPFVPDDAA
                     RLAQDCFEAYSIQVSGLEQRLRSIGRPTVVIGVSGGLDSTHALIVACRAMDRLGRPRS
                     DVLAFTMPGFATSDHTKGNAIRLMESLGVTWEELDIRPAATQLLRDLGHPVGRGEEVY
                     DVTFENVQAGLRTDYLFRIANQRKGIVLGTGDLSELALGWCTYGVGDQMSHYGVNTGV
                     PKTLIQHLIRWVIASGQFSDEVGATLRSILDTTITPELVPTREGEAAQSTEAMIGPYA
                     LQDFTLYYVLRHGLRPSTIAFLAWHAWHDTASGQWPEGFPEGARTAYTLGEIRHWLEV
                     FLTRFFANQFKRSALPNGPKVVRGGSLSPR"
     gene            2208..3452
                     /locus_tag="PLB86_09925"
     CDS             2208..3452
                     /locus_tag="PLB86_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRC65494.1"
                     /translation="MPPAPAPSRGPRFEPFRALRYAPGIDLDAVIAPPYDVLSDADVA
                     ALRARDPHNIVHADIPAGTDPTRYAASAHLLRDWQETGVLVRDEVPTLSIYRMRFVDE
                     AGQRRELVGVLGGLEVVAEGAGRVLPHERTTPKASTDRLDLTLATATNLSPVWGLSLA
                     QGLTAALAEPAEPMGSLVVDGVEHVVERVVDPDRIAAISAIIGADDVLIADGHHRYGV
                     ARRYRDEMAEAKEPEGAAGAGATLAFVGELVADQLAIDAIHRIVRGLTPAALREALAR
                     DFELVPAPAAQPGPALLAELNRSGRLLLIESPEVATWLVPRPGAFDDVRALDGAWLEH
                     TLAGTGAVLDYQHGVAEVLDVLHADPQALAILIRPTSLEQIERTAREGLLMPPKSTFF
                     TPKLRTGLVLRPLDEAGYVATA"
     gene            complement(3449..4495)
                     /locus_tag="PLB86_09930"
     CDS             complement(3449..4495)
                     /locus_tag="PLB86_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRC65495.1"
                     /translation="MSLAIGSLLVITVYGVVGYMILGYNLLDAIYQAVMTLTTVGYGE
                     VLEATPVRKAFSTSLMILGVGIALYNLTVMVGTVTEGDVRKYFERRRMDKRISGMSGH
                     VIICGHGRVGRSAREHLKNIGSQVVVVDNVPDRVMHLEDPHLIGDCTRDEVLRAAGIE
                     RARALIATLETDADTVYLTLSARALRPDLVIIARARTVDSKEKLVLAGATRAVNPQLI
                     GGRRMAAFALNAHVAEFLDVVMHDEELDFRIEQIRIPVDSPLAGRSLGELDLEQSSGA
                     RLLAHRPGPRMPFAPHPSADTVLEPGSVLIAFGTMAQIEHLMDQASPDPGTLGAGPAA
                     PGGPVSPIIARTSA"
     assembly_gap    4535..4634
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4813..>6112
                     /locus_tag="PLB86_09935"
     CDS             4813..>6112
                     /locus_tag="PLB86_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC65496.1"
                     /translation="MGSADRDVSRVAIVGAGPSGLFAAQSLVGQSDLPVQVDLIDRLP
                     TPYGLLRYGVAPDHTSIKSVANALARTFDSSGVRFIGNVELGRDISREQLLAAYDAVV
                     YAAGAAEDAQLGVPGEDLAGSRSAREFVAWYSGHPDAPAQDLGGVRTAAVIGVGNVAV
                     DVARVLAKTADSLEVTDMPPAVLAELHRHQADDIHVIARRGPQHAAFTTVELRELVHT
                     PGLAVSVNEDAFDGIPDDLERRPKANVEILREAAAKVVENPRRRLHFWFWRKPIAIEG
                     DGRVAALVLERTRLDETGKVVGTGETQRLATELVLRSIGYRSTALAGVPFDERAAVIP
                     NVEGRIVGADGEVLPREYVVGWIKRGPTGVIGTNKSDASQTVRHLVADLVAARAGDGG
                     AAGGVTGGVTGGVTGADRPDLPTALAEQGHEVISLDGWRAI"
BASE COUNT          929 a         2066 c         2135 g          882 t
ORIGIN      
        1 cggggcgaca gcgaaccgcc gcggaccacc ttcgggccgt tgggcagcgc cgagcgcttg
       61 aactggttgg cgaagaaccg ggtgaggaag acctccagcc agtgccggat ctcccccagg
      121 gtgtatgccg tgcgcgcacc ttcggggaaa ccttccggcc actgcccgct cgccgtgtcg
      181 tgccaggcat gccaggccag gaaggcgatc gtcgacggtc gcaagccgtg gcgcaggacg
      241 taatacagcg tgaaatcctg cagggcatag ggcccgatca tggcctcggt cgactgggcc
      301 gcctcaccct cacgggtcgg caccaactcg ggcgtgatcg tcgtgtcgag gatcgagcgc
      361 agggtcgccc ccacctcatc gctgaactgg cccgaggcga tgacccaccg gatgaggtgc
      421 tggatgagcg tcttgggcac ccccgtgttg accccgtagt gcgacatctg gtcaccgacg
      481 ccataggtgc accaccccag cgcgagctcg gacaggtcac ccgtcccgag cacgatcccc
      541 ttgcgctggt tggcgattcg gaacaaatag tcggtgcgca ggccggcctg gacgttctcg
      601 aaggtgacgt catagacctc ctcgccgcgc cccaccgggt gcccgaggtc gcgcagcaac
      661 tgggtggccg ccggccggat gtcgagctcc tcccaggtga ccccgagcga ctccatgagc
      721 cggatcgcgt tgcccttcgt gtgatccgag gtcgcgaagc ccggcatggt gaaggccagc
      781 acgtccgagc ggggccgccc caaccggtcc atagcccggc acgcgacgat cagcgcgtgg
      841 gtcgagtcga gcccacccga caccccgatg accaccgtcg gccggccgat cgagcgcagc
      901 cgctgctcta gccccgacac ctggatcgaa tacgcctcga aacagtcctg cgccaaccgg
      961 gccgcgtcgt ccggcacgaa cggatagcgg tcgactgccc gacgcagccc gagatcgccc
     1021 cgcggcggat ccaaccggaa ggtcacctcg cggaacgccc cgacccgcgc cgcgtgagtg
     1081 cgccggttgt cgtcgaacgt cccctgacgc atccgctcct gccgcagcct cgccaggtgc
     1141 acgtcggcga ccgaccgtcg cggccccgac gggaagcgct cgccctcggc cagcagcgac
     1201 ccgttctccc agaccatggt ctggccgtcc cacgagaggt cggtgctcga ctcgccttct
     1261 ccggcagcgg catacaggta tgccgcgagg cagcgtgagg aggccgactc ggcgagcatc
     1321 gcgcggtcgc gcgccttggc gaccgtgatc ggcgaccccg acacgttgac gagcacggtg
     1381 gctccggcga gcgcggcctc ggccgacggt gggatcggga cccacatgtc ctcgcagacc
     1441 tcgacgtgga cgacgaggtc aggcacatcg acggcccgga agagcagatc cggcccaaac
     1501 ggcacgtcgg cctcgccgac ccgaatggtc ccgcgcacgt cgtcgccggc cgcgatggtc
     1561 cgcgactcat agaactcgcg ataggtcggc aggtaggact tcggcgccac cccgagcacc
     1621 cgcccccggt gcacgacgac ggcgcagttg tagacccgcc cacgtgcccg caggggcgcg
     1681 ccgacgacca gcacgggcag caactcggcc gatgcgcgca ccaactcgtc gagcgcgacc
     1741 tcgactgcgt cgagcagtgg gtcggccagc acgaggtcgt ccagcgagta gccggtcagc
     1801 cccagttccg ggaacaccgc gacggcaacg ccctcgtcgt ggcactcgcg cgccacggcc
     1861 aacgtggcgg cgacattggc ggccgggtcg acgagacgca ccgggaccgt gcaggcagcg
     1921 acccgcgcga agccgtggtc atagatggag aagaagttca tgcggctacc gcgcgcctcc
     1981 ctggtccggc ggccccgggt cggagcccgt cccgagtctc ccacgacccg agggcatggg
     2041 tatgccgcgt gcggtgaggc caggggcatg gcccgagcga tggacacgag ggggagcgcc
     2101 accgatcgcc gacacgcggc atacataagc attgtgtccc acgtcttgca gttccgcatc
     2161 tcgaactgct cacggaggca ggctcgctcg ttgcctagac tcctggtatg ccgcccgcac
     2221 ccgccccttc acgcgggcct cggttcgagc cgttccgggc tctgcgttac gcgcccggaa
     2281 tcgacctcga cgccgtcatc gcaccgccct acgacgtgtt gtccgacgcc gacgtcgccg
     2341 cacttcgggc tcgcgacccg cacaacatcg tccacgcgga catccccgct ggcaccgacc
     2401 cgacccggta tgccgcctcc gcgcacctgc tgcgcgactg gcaggagacc ggcgtcctgg
     2461 tccgtgacga ggtgccgacg ctgtcgatat atcggatgcg tttcgtcgac gaagccggcc
     2521 agcgacgcga gttggtcggg gtcctcggcg ggttggaggt ggtcgccgag ggggccggtc
     2581 gggtgcttcc gcatgagcgg accacaccca aggcgtcgac cgaccgtctc gatctgaccc
     2641 tggccaccgc caccaacctc tcgccggtct gggggctgtc gctggcgcag ggtttgaccg
     2701 ctgcgctcgc cgagcccgcc gagccgatgg ggagtctggt cgtcgacggc gtcgaacatg
     2761 tcgtcgagcg ggtcgtcgac cccgaccgga tcgccgcgat ctcggcgatc atcggcgccg
     2821 acgacgtgtt gatcgccgac ggtcaccacc gttatggcgt ggcccggcgc taccgcgatg
     2881 agatggcgga ggccaaagag cccgagggcg cagccggggc gggggccacg ttggccttcg
     2941 ttggagagct cgtcgccgat cagctggcca tcgacgccat ccaccgcatc gtccggggcc
     3001 tgaccccggc cgccttgcgg gaggcgttgg cgagggactt cgagttggtg cccgcccccg
     3061 ccgcccagcc cggcccggcg ctgctcgccg agctcaaccg ctcagggcgg ttgttgctca
     3121 tcgagtcacc ggaggtggcg acctggttgg tgccgcggcc gggggctttc gacgacgtgc
     3181 gggcactcga tggcgcctgg ctggagcaca cgctggccgg gacaggggcg gtgctggact
     3241 accagcacgg ggtggccgag gtgctcgatg tgctgcacgc cgacccgcag gccctggcga
     3301 tccttatccg cccgaccagc ctcgagcaga tcgagcggac ggcgcgcgag ggcctgctca
     3361 tgccacccaa gtcgacgttc ttcacaccca aactgcgcac cggccttgtg ctgcgccccc
     3421 tcgacgaggc tggatacgtc gcgactgcct aagccgacgt gcgggcgatg atgggggaga
     3481 cggggccacc gggggcggcg ggccctgccc cgagcgtccc ggggtcggga gaggcctgat
     3541 ccatgaggtg ctcgatctgg gccatcgtgc cgaacgcgat caacaccgac ccgggctcca
     3601 gcacggtgtc cgccgacggg tggggggcga acggcatacg cgggcctggt cgatgggcga
     3661 gcaggcgcgc gccggacgat tgctcgaggt ccaactcgcc gaggctgcgt ccggccaacg
     3721 gcgagtcgac tgggatccgg atttgttcga tccggaaatc gagctcctcg tcgtgcatga
     3781 cgacgtcgag gaactccgcg acatgggcgt tgagcgcgaa ggccgccatg cggcgaccgc
     3841 cgatgagttg cgggttgacg gctcgggtcg ccccggcgag gacgagcttc tccttggagt
     3901 cgacggtgcg ggcgcgggcg atgatgacca gatcgggacg cagggcccgc gccgagaggg
     3961 tgagatagac ggtgtcggcg tcggtctcca gggtggcgat gagggcacga gcgcgctcga
     4021 tgccggcggc gcgtagcacc tcgtcgcgcg tgcagtcgcc gatgaggtgc gggtcctcga
     4081 ggtgcatgac ccgatcgggg acgttgtcga cgacgaccac ctgcgaaccg atgttcttca
     4141 ggtgctcgcg cgccgagcga cccacccggc cgtgaccaca gatgatgacg tggccactca
     4201 tgcccgagat tcgcttgtcc atccgacgcc tttcgaagta cttccggaca tccccctcgg
     4261 tgaccgtccc gaccatcacc gtgaggttgt aaagagcgat accgaccccg aggatcatca
     4321 ggctggtcga gaacgccttg cgcaccggcg tcgcttccaa gacctcgcca tagcccacag
     4381 tcgtcagggt catgaccgct tggtagatcg cgtcgagcag gttgtagccg aggatcatgt
     4441 aacccacgac gccatagacc gtgatgacaa gcagcgaccc gatcgcgagg gacatgcggc
     4501 ggacgatgct cacgttggct attgaaccgc gtccnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnggacga tgctcacgtt ggctattgaa ccgcgtccag ggcgttccgg
     4681 atagcagcgc aggtatgccg cgcggcatac ctcgtccggc gtgtaggtgg gacctcggtc
     4741 acgcgacgtt cgcggcggtg cccgttaggc tcgggccgtc aacgtcgacg ctcgtggcac
     4801 cggaggtgcg cgatgggcag tgcagatcgg gacgtcagcc gggttgcgat tgtcggggcc
     4861 ggcccgtccg ggctgtttgc cgcccaatcg ctggtcgggc agagcgacct gccggtgcag
     4921 gtggacctca tcgaccggct gccgacgccc tacggcctgc tgcgctacgg cgtcgcccct
     4981 gaccacacga gcatcaagtc ggtcgccaac gcgctggccc gcaccttcga cagcagcggc
     5041 gtccgcttca tcggcaacgt cgagctcggt cgagacatct cgcgcgagca gcttctcgcg
     5101 gcctacgacg ccgttgtgta tgccgcgggc gcggctgagg atgcccagct cggcgtcccg
     5161 ggggaggacc tggcgggcag ccggtcggcg cgcgagttcg tcgcttggta ttccgggcat
     5221 ccggacgccc ccgcgcagga cctcggcggc gtgcggactg cggcggtgat cggcgtcgga
     5281 aacgtcgccg tcgatgtcgc ccgggtcctg gccaagacgg ccgactcgct cgaggtgacc
     5341 gacatgcccc cggcggtgtt ggctgaactg caccggcacc aggccgacga catccacgtc
     5401 atcgcgcggc gcggtccgca gcacgcggcc ttcacgacgg tcgagctgcg cgagctcgtc
     5461 cacacgccgg ggctggcggt gagcgtcaac gaggacgcgt tcgacggcat acccgatgac
     5521 ctggagcgcc gaccgaaggc gaacgtcgag atcctgcggg aggcggccgc gaaggtcgtc
     5581 gagaacccgc gtcggcggct gcacttctgg ttctggcgca agccgattgc catcgagggc
     5641 gacggccgag tcgcggcgct cgtgctggag cggacccggc tggacgagac cggcaaggtc
     5701 gtcgggaccg gcgagaccca gcggctggcg accgagttgg tgctgcgctc gatcgggtat
     5761 cggtcgaccg cgctggcggg ggtgccattc gacgagcgtg ccgcggtgat ccccaacgtc
     5821 gaggggcgga tcgttggggc ggacggggag gtgctgcctc gggagtatgt cgtcgggtgg
     5881 atcaagcgcg gcccgaccgg ggtcatcggc accaacaagt cggatgcgtc gcagaccgtg
     5941 cgccacctcg tcgccgactt ggtggccgct cgggccggcg atggcggggc ggccggtggg
     6001 gtgactggtg gggtgaccgg tggggtgacc ggggcggacc gaccggacct gcccactgcg
     6061 ttggccgagc agggccacga ggtgatcagc ctcgacgggt ggcgggccat cg
//
LOCUS       DAORPW010000134         6097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3496_length_6097_cov_757.303045, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000134 DAORPW010000000
VERSION     DAORPW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3496_length_6097_cov_757.303045"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLB86_09940"
     CDS             <1..599
                     /locus_tag="PLB86_09940"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HRC65497.1"
                     /translation="ALLVGVAGWVAVSAARPAPVEGGIPVLVAVRDLAAGARVGSGDL
                     RAVRVPPDAVPARAILDASAAAGALLAGPLSEGEVLTATRLQGPGLLAGASSDTVAVA
                     VPLADPSILAALRPGDRVRVLAVGTGAVVGDATVLVATAPTDEVSGLVATGPGSGSLI
                     AALDRQAASAMAAAQGPSGVSGGFVIALLNSDTIRAPM"
     gene            781..1146
                     /gene="mscL"
                     /locus_tag="PLB86_09945"
     CDS             781..1146
                     /gene="mscL"
                     /locus_tag="PLB86_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRC65498.1"
                     /translation="MLKGFKDFLMRGNVVDLAVAVIIAGAFGKVIEAFVTIVMDIIGK
                     LGGQPDFSAWAPGGVHLGSFITVTISFLIIAAAVYAIVVVPLNKLAERRKAGEVVEDE
                     APSAEVALLTEIRDALRTR"
     gene            1364..2086
                     /locus_tag="PLB86_09950"
     CDS             1364..2086
                     /locus_tag="PLB86_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRC65499.1"
                     /translation="MTWLVLGIALIAAFSAFTQIITGFGFALVGLPLLALVTDPVTAV
                     VSMTVLSTALTLGASIQQRRDIEWRPAGSVTATALLGMPVGLLALRLVDARWLSVLIG
                     IVLLAFTFALVRGIRLQGRAVTPTAGFVSGALLMSTGMNGPPLVVAFQNKEMSPPQFR
                     ATLQAVLTIQGFFGLVGFAAVGLFSPPVWSVVAYGAPAMLVGWWVGHLVSRRMDAALF
                     RRLVLVTLAASAATSIATGLLG"
     assembly_gap    2261..2360
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2361..6067)
                     /locus_tag="PLB86_09955"
     CDS             complement(<2361..6067)
                     /locus_tag="PLB86_09955"
                     /inference="COORDINATES: protein motif:HMM:NF037405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC65500.1"
                     /translation="MSVAQTIASWARELSEVGGRNTLLWAPEGPDRDGYLDLTTAHPG
                     GVSMLLAGRPTSLSDLVREAAAFEEARDTVRAIHAKASELLNERGLVVGFVAIGVATW
                     DPPRARAIVEAPVLLRTCTLRPTSPAQHDFDLDLGPTVEVNPVLVNYLRSVAGIAVDP
                     TALAALTTLKGSAGGFDPYPVYAALGRLCADLPGFAVTPRLVLGTYPYGKPDMVADVS
                     GQAQWLAQVDLVAALAGDEPAITRLRQALPEPAETPDPELEMRVLDLDATQESVIEAV
                     RAGRQLVLTAPTGTGATQTVAALVAALAHDGKQVLYVTSQRRSLRELQDRLASVGLDD
                     LVLDLTGAGEDRRAVTSELGQALARLATLDDRDLADAADPRGRLDRAATVGQSEQTLA
                     DHVAAIHEVRRPWGVTAYEIQEAMGALAARHPAPGSRVRIGGAALEELSRARVTELAE
                     HLQAAAAAGAWSAEGGDPWYGATIRSEAEVERARDIVTRLTGGDLAATTADLDSILVE
                     SSLRPARSAADWRHALVTMRGVHETLEVFRPEIFDVPLDEHVVATGSRAYRAESAVEL
                     GGWSRSRVRRQARRMLRPGRPPADLHAELVRARNQRIAWHDLVGAGGRPEISPRLEEG
                     EAAYQGLVADLTWLDERLRPGPDDGPMLTTSFPLLRARLTRLAERLDRLDILPQVTAV
                     VDELRAARMGEVMDDFAARGVAAEHVPAEVEHVWWASLGQEIRARDPRYANHDGGALR
                     LAADQLAGLDEVGRTSDAGRVRAVVDRRARQRAREHPRQTDLLHAQAGLGHGHLPFAA
                     LHREADRVIGALRPCLAVSPYAVAQVLAPGTAVDVVVLDDATTVSVAEAVSALSRGRQ
                     TLVIGRPDGAVPSAFRASAGTRPVTDPSAAPPSLYAAATAVLPERRLAWWHTDLDARL
                     ALLPPEGITHGAPSPLRVPALRLDHVEGTAAVLPGTDSAIEWTAAEVARVVELVLEHA
                     RHRRDQTLAVLTLTADMAHLVRDGVRDGIGRLDGDVAAGEFLAGVGVEPFVVARVEEA
                     DGLLRDVVLLAVGYGRTPHGRVLHRFPALNLPGGEAALIAATARARQELVVVSTLRSG
                     DLDAGRLRATPAGRLVELLAHAEAGGIHEGESTTAQGGDPLMSYLASRLRQEGLTVQA
                     QMGVGPHRVELAVGHPSVADRWLVAVESDGPGYAALPGVRARDVLRPRQLRRLGWEPI
                     RVWSTDLYRDPAPEVVRVVAAVTATLRARNA"
BASE COUNT          772 a         2082 c         2310 g          833 t
ORIGIN      
        1 cggcactcct cgtgggggtc gccgggtggg tggccgtgtc ggcggcccgc cccgctcccg
       61 tcgaaggcgg cataccggtg ctcgtggcgg tccgagacct ggcagcgggg gcgcgggtcg
      121 gatcagggga cctccgcgcg gtgcgggtgc cgccggacgc ggtcccggcg cgggcgatcc
      181 tcgacgcgag cgccgcggcg ggcgccctcc tcgccggccc gctgagcgag ggcgaagtgc
      241 tcactgcgac ccgcctgcag ggtccaggcc tgctcgccgg ggcgtcgtcc gacacggtcg
      301 cagtggccgt cccgttggcc gatccatcga tcctcgcggc cctgcgaccc ggagatcgcg
      361 tccgggtgct ggccgtgggc actggcgccg tcgtcggcga tgcgaccgtc ctcgtcgcga
      421 cggcgccgac cgacgaggtc agcgggctgg tcgcgacggg tcctggctcc ggcagtctta
      481 tcgcggccct ggaccggcag gcggcctcgg ctatggcggc tgcccaaggc ccgtcggggg
      541 tgtctggggg gttcgtcatc gcgctgttga actccgacac gatacgcgcg ccgatgtgac
      601 ctaattccca ttttgtgcac ggacggagta gcctgaacgt cgtgctcggg gaagctctcc
      661 tctggcatgc caagcgaaac acttcatgct cccctgggcg cacctgccgc ccacgccacc
      721 ctcaactgag gctggatctc ccagggtcgg gagcccaccc cacaaaagga tcacgcaccc
      781 atgctcaagg gattcaaaga cttcctcatg cgcggcaacg tcgtcgacct cgcggtcgcc
      841 gtcatcatcg ccggcgcgtt cggcaaggtc atcgaggcct tcgtcacgat cgtcatggac
      901 atcatcggca agctcggcgg tcagccggac ttctccgcat gggccccggg cggcgtgcac
      961 ctcggcagct tcatcaccgt gacgatctcg ttcctcatca tcgcggccgc cgtctacgcc
     1021 atcgtcgtcg tcccgctcaa caagctcgcc gagcgccgca aggccggcga ggtcgtcgag
     1081 gacgaggccc cctcggccga ggttgccctc ctcaccgaga tccgcgacgc cctgcgcact
     1141 cgctgatctc ggcctcgcag gacagcacca tcggcacgac ctgaaccact ggcacgacct
     1201 gaaccacacg caccacctgt tcgaccacga aacgccccgg cggggcggga gccattggca
     1261 cccgccccgc cgtcggcttt cacgggcggg taggcctctg ggaccacgtg cccacatcgt
     1321 ccgaccgggt gccaacctca cgctgggaag gcacacttga tgggtgactt ggcttgtcct
     1381 cggcatcgct ctgatcgccg cgttcagcgc gttcacccag atcatcaccg gattcggctt
     1441 cgccctcgtc ggccttccgc tcctcgccct cgtgaccgac cccgtgacgg cagtggtctc
     1501 gatgactgtc ctgagcactg cgctcaccct cggcgcctcg atccagcagc gccgcgacat
     1561 cgaatggcgc ccagccggaa gcgtcacggc cactgccctg ctgggtatgc cggtcgggct
     1621 gcttgccctg cgcctcgtcg acgcgcgctg gctcagtgtc ctcatcggca tcgtgctgct
     1681 ggcgttcacc ttcgcgctcg ttcgcgggat ccgattgcag ggtcgcgccg tgaccccgac
     1741 cgcgggcttc gtcagtggcg cgctgctcat gtcgacgggc atgaacggcc cgcccctggt
     1801 cgttgcgttc cagaacaagg agatgagccc gccgcagttc cgcgccaccc tgcaggcggt
     1861 cctcacgatc caaggcttct tcggcctcgt aggcttcgcc gccgtggggt tgttcagccc
     1921 gcccgtctgg tcggtcgtgg cctacggagc gccggcaatg ctcgtcggct ggtgggtcgg
     1981 gcatctcgtc tcccgacgga tggatgccgc gctgttccga cggctcgtcc tggtcacgtt
     2041 ggccgccagc gcagccacct cgatcgccac cggcctgctc ggctgagcag gcccgagcgg
     2101 catacctgcc gggtcactcc cagtgcggag ggcgctgttc ctcgagccaa cggtcgtgct
     2161 cgtcgccgct gcggggccgc tcgccccacc cggtgtcggt gtcgtcggcg ctgcgctcgg
     2221 cagaactgcg cttgagcttc ttgcgcttgc ccggcttggc nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn tgggcgttcc gcgcccgcag cgtggccgtc acggcggcga
     2401 caacgcgcac cacttcgggc gccgggtcgc gatagagatc ggtgctccag acccgaatcg
     2461 gctcccagcc gagtcggcga agttgccgcg ggcgcaggac gtcgcgggcg cggacgcccg
     2521 ggagggcggc atacccgggt ccgtcgctct ccaccgcgac cagccagcgg tcggcgacgg
     2581 aggggtggcc gacggcgagt tcgacgcggt gggggccgac acccatctgg gcctggacgg
     2641 tgaggccctc ctggcgcagc cgcgaggcga ggtaggacat gagcgggtcg ccaccttggg
     2701 cggtggtcga ctccccctcg tgtatgccgc ccgcctcggc gtgcgcgagc aactcgacca
     2761 ggcggccggc cggggtcgcc cgcagccggc cggcgtcgag atcgccggag cgcagcgtcg
     2821 acacgaccac gagttcctgc cgggcccgcg ccgtggcggc gatgagggca gcttcgccac
     2881 cgggaaggtt gagcgccggg aagcggtgca gcacccggcc gtgcggcgtg cggccgtagc
     2941 cgacggcgag cagcacgacg tcgcgcagca ggccgtcggc ctcctcgacc cgggccacga
     3001 cgaacggctc gaccccgacg ccggcgagga attcgcccgc cgcgacgtcg ccgtcgagcc
     3061 ggccgatccc gtcgcgaacg ccgtcgcgca ccaggtgggc catgtcggcg gtgagggtga
     3121 ggacggccag ggtctggtcg cggcggtgcc gggcgtgctc caggacgagc tcgacgaccc
     3181 gcgcgacctc ggcagcagtc cactcgatgg cgctgtcggt gccgggcagc acggccgcgg
     3241 tgccctcgac atggtcgagg cgcagcgcgg ggactcgcag cggcgacggg gcaccgtggg
     3301 tgatcccctc cggaggcaac aacgcgagtc gggcgtcgag gtcggtgtgc caccaggcga
     3361 ggcgacgctc gggcaggacg gcggtggcgg cggcatacag ggagggcggg gcggccgacg
     3421 ggtcggtgac gggacgggtt ccggcgctgg cgcggaaggc gctcgggacg gctccgtcgg
     3481 gacgaccgat gacgagcgtc tggcggcctc gggacagcgc cgacacggct tcggcgacgc
     3541 tgaccgtggt ggcgtcgtcg aggacgacca cgtcgacggc ggtgccgggg gcgaggacct
     3601 gcgcgacggc atacgggctg acggccaggc acgggcgcaa ggcgccgatg acccggtcgg
     3661 cctcgcggtg cagggccgca aacggcaggt gcccatggcc gagccccgcc tgcgcgtgca
     3721 ggaggtcggt ctggcggggg tgctcgcgag cgcgctggcg ggcgcgacgg tcgacgaccg
     3781 cgcggacccg gcccgcgtcg ctcgtgcgcc cgacctcgtc gagcccggcg agttggtcgg
     3841 cggccaggcg cagcgcgccg ccgtcgtggt tggcgtagcg cgggtcgcgg gcgcggatct
     3901 cctggcccag cgacgcccac cagacgtgct cgacctcggc gggcacgtgt tcggcggcga
     3961 ccccgcgtgc ggcgaagtca tccatcacct cgcccatccg cgcggcccgc aactcgtcga
     4021 cgaccgcagt gacctggggc aggatgtcga gccggtcgag ccgctcggcc agccgggtca
     4081 ggcgggcgcg cagcagcggg aacgaggtcg tgagcatggg cccgtcgtcg ggtccggggc
     4141 gtagccgctc gtcaagccag gtgaggtcgg caacgagccc ttggtatgcc gcctcgccct
     4201 cctccaaccg cggggagatc tcgggccggc cgccggcgcc gacgaggtcg tgccaggcga
     4261 tccgttggtt gcgggcgcgg accaactcgg cgtgcaggtc ggccgggggg cgaccgggac
     4321 ggagcattcg gcgggcctgg cggcgcacgc gcgagcgcga ccagccgccg agctcgacgg
     4381 ccgactcggc gcgataggcg cggctgccgg tggcgacgac gtgctcgtcg agcgggacgt
     4441 cgaagatctc gggccggaag acctcgaggg tctcgtggac gccgcgcatg gtgacgaggg
     4501 cgtggcgcca gtcggcggcg gagcgggccg ggcgcaggct ggactcgacg aggatcgagt
     4561 cgagatcggc ggtcgtcgcg gccaggtcgc cgccggtgag ccgcgtgacg atgtcgcggg
     4621 cccgctcgac ctcggcctcg gagcggatgg tggcgccgta ccaggggtcg ccgccctcgg
     4681 ccgaccaggc tccggcggcg gctgcggcct gcaggtgctc ggccaactcg gtgacccggg
     4741 cgcgggagag ctcttcgagg gcggcgccgc cgatccgcac ccgggagccc ggcgcggggt
     4801 gacgcgcggc gagggcgccc atcgcctcct ggatctcgta tgccgtgact ccccacgggc
     4861 ggcgcacctc gtggatcgcg gcgacatggt cggcgagggt ctgctcggac tgcccgacgg
     4921 tggccgcccg gtcgaggcgc ccgcgagggt cggcggcgtc ggcgaggtcg cggtcatcga
     4981 gggtcgcgag gcgggcgagt gcttggccta gctcgctcgt gacggcgcgg cgatcctcac
     5041 cggcgccggt gaggtccagg acgaggtcgt cgaggccgac ggaggcgagc cggtcctgca
     5101 gttcgcgcaa cgaccgacgc tgggaggtga cgtagaggac ctgcttgccg tcgtgggcga
     5161 gggcggcgac gagggcggcg accgtttggg tggcaccggt gccggtcggg gcggtgagga
     5221 cgagttggcg gcccgcgcgg accgcctcga tcaccgactc ctgggtggcg tcgaggtcga
     5281 ggacccgcat ctccaactcg gggtcggggg tctcggcggg ctcagggagg gcctgacgca
     5341 gccgggtgat ggccggctcg tccccggcga gggcggcgac gaggtcgacc tgggcgagcc
     5401 attgggcctg accgctgacg tcggcgacca tgtcgggctt gccgtagggg taggtcccga
     5461 gaaccaacct cggggtgacg gcgaagccgg ggaggtcggc gcagaggcga ccgagggcgg
     5521 catacacggg gtaggggtcg aagccacccg cggagccctt gagcgtcgtg agcgcggcga
     5581 gggcggtggg gtcgacggcg atgcccgcga ccgagcgcag gtagttgacg agcacggggt
     5641 taacctcgac ggtcggccca aggtcgaggt cgaagtcgtg ctgggccggg ctggtggggc
     5701 gcagggtgca ggtgcgcagc aggaccgggg cctcgacgat cgcgcgggcg cgcggcgggt
     5761 cccaggtggc gacgccgatg gcgacgaacc cgacgaccag gccgcgctcg ttgaggagct
     5821 cggaggcctt ggcgtggatc gcccggacgg tgtcgcgggc ctcctcgaag gcggcggcct
     5881 cgcgcaccag gtcggacagg ctggtgggac ggccggcgag cagcatcgag acgccgccgg
     5941 ggtgggcggt ggtgagatcg aggtagccgt cgcggtcagg cccctcgggc gcccagagca
     6001 gggtgttgcg gccgccgacc tcggacagct cccgggccca gctggctatc gtctgcgcaa
     6061 cactcaccgg gtcaggctaa ccggggcagc gcggttg
//
LOCUS       DAORPW010000135         6056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3536_length_6056_cov_734.157640, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000135 DAORPW010000000
VERSION     DAORPW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3536_length_6056_cov_734.157640"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLB86_09960"
     CDS             complement(<1..200)
                     /locus_tag="PLB86_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:1990904 - ribonucleoprotein complex
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRC65501.1"
                     /translation="MSIHEKNPRDILIAPVVSEKSYGLLDEGKYTFVVDPRANKTEIK
                     IAVEAVFGVKVDKVHTLNRQGKT"
     gene            complement(197..1210)
                     /gene="rplD"
                     /locus_tag="PLB86_09965"
     CDS             complement(197..1210)
                     /gene="rplD"
                     /locus_tag="PLB86_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRC65502.1"
                     /translation="MATTTALPADIFDVQVNVPLIHQVVAAQLAAARQGTHSTKTRGL
                     VSGGGKKPYRQKGTGRARQGSTRAPQFAGGGVVHGPQPRDYSQRTPKKMKAAALRGAL
                     SDRARNGRIHVIDVLVSGDAPSTSTAAAVLAGLTDRTKFLVVLERGDQVGLKSVRNLD
                     SVHVLAVDQLNTYDVLCADDVVFTAGALATLTASSKAAATAPVAATVEKADKAEKPAK
                     VEKAEKPAKAEKAASLVDAAEAAPAPKATKAAKVAEAPAEAAAELPAAAAAPLADGSA
                     PEGYSVKGNADSGLYHEPDGRWYDATVAEFWFKSAADAEAAGFKKAGSRAKSTAKDGE
                     DAK"
     gene            complement(1216..1869)
                     /gene="rplC"
                     /locus_tag="PLB86_09970"
     CDS             complement(1216..1869)
                     /gene="rplC"
                     /locus_tag="PLB86_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRC65503.1"
                     /translation="MSNATTRTVKGVLGEKLGMTQVWDADNRLVPVTVIKVGPNVVTQ
                     VRGDADGYPAVQIGYGEIDPRKVNKPMKGHFERAGVTPRRHLVELRTADASDYALGQE
                     ITVSTFEAGQLVDATATTKGKGFAGVMKRHGFHGVSSSHGAHRNHRKPGSIGACATPG
                     RVFKGVRMAGRMGGERQTTQNLAIQAVDADKGLLLIKGAVPGPRGAIVLVRSASKGA"
     gene            complement(1880..2188)
                     /gene="rpsJ"
                     /locus_tag="PLB86_09975"
     CDS             complement(1880..2188)
                     /gene="rpsJ"
                     /locus_tag="PLB86_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRC65504.1"
                     /translation="MAGQKIRIRLKSYDHEVIDSSARKIVDTVTRAGATVVGPVPLPT
                     EKNVFVVIRSPHKYKDSREHFEMRTHKRLIDIIDPTPKAVDSLMRLDLPADVNIEIKL
                     "
     gene            complement(2536..3417)
                     /locus_tag="PLB86_09980"
     CDS             complement(2536..3417)
                     /locus_tag="PLB86_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRC65505.1"
                     /translation="MTPRDRLLALAVVTVWGLNFVVIDWGLPGVPPLVFVTMRFVLVL
                     LPAIFFVPRPAGRWQDIALVGLLLSVGQFGLLYSAMSAGMPPGLASLVLQIQAAFTVV
                     FAATLLRERPSRRQVIGVGLGLLGLGIVALGRGGSVPLGALGLCVAAAASWAGGNIAV
                     RRLPGVGGLSLTVWSGLVVPIPLFALALLVEGPETVMHALTHLSVPSLLSTAYTAVLA
                     SLFGYGVWNSLMGRHRAAEVAPYSLLVPVVGMTAALVFRGERPGLLALVGGALLVAGV
                     AVTTLRRRQVAGELVSR"
     gene            3490..4413
                     /locus_tag="PLB86_09985"
     CDS             3490..4413
                     /locus_tag="PLB86_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRC65506.1"
                     /translation="MIDLAAVRTLDAVREHGSVVAAAAALGYTPSAVSQQIKRLERHT
                     GVQLLERVGRGVALTELGRRLADDGNRVLGDLEAIEAQLAAAHGSAPLHGQVRIAAFS
                     TGVRGLVAPALATLRHTDPGLDVRVVESDPHEALDLVAGGQVDTALVHNWGDLPLPIP
                     AHVEDVHLGIDTADVLVPRDHPLATRSVITAADLDGLVWTCAPVGSVCHGWLVHLHDA
                     HGLRPDVRYWAMEFSSQIGLVEHGNAVALVPRLGRESLPSGVIAVPVDDPVTRRILQV
                     WRRTMAPSPAIRRVHDVLTSAMDAVAVAHPR"
     gene            complement(4504..5838)
                     /locus_tag="PLB86_09990"
     CDS             complement(4504..5838)
                     /locus_tag="PLB86_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HRC65507.1"
                     /translation="MHTAFDPDSTAQGLIDFVDASPTPFHAVDSAIRLLAAEGFTALD
                     ESAPFPSQPGRYYIARGGALVAWIETGGMPPTAPFRVIGTHTDSPNLRVKPRPDLVKA
                     GWQMVAVEPYGGLLINSWLDRDLGLAGRVAVRDNSAPTGVSTRLFHDRAPLLRVAQLA
                     IHLDRGVNERGLVLNPQQHLVPLWGVGEAPASVAAYLGEQVGADPGDVLAWDAMLHDT
                     QPSARIGRAQELIAAPRLDNLASSYAAVRALLAVAASGGTSADQEADAAYRPVIVLFD
                     HEEVGSRSERGADSPMLRGILERIVLAAGGGRDDLWRALAGTIVASADMAHATHPNYS
                     ELHEPGHRIEMNGGPVLKVQTELRYATDAVGAAALHLACEQADVPLQVFVTRSDLPCG
                     STIGPITAGQVGVTTVDLGAPMLSMHSIRELAGVADQAMYAAALAAFLAPVP"
BASE COUNT          895 a         2112 c         2145 g          904 t
ORIGIN      
        1 gtcttgccct gccggttgag ggtgtggacc ttgtcgacct tgacgccgaa caccgcctcg
       61 acggcgatct tgatctcggt cttgttggcg cgggggtcga cgacgaaggt gtacttcccc
      121 tcgtcgagca ggccgtagga cttctcggag acgaccgggg cgatcaggat gtcgcgcggg
      181 ttcttctcgt ggatgctcac ttggcgtcct ccccatcctt ggccgtgctc ttggcgcggc
      241 tgccggcctt cttgaagcct gcggcctcgg cgtcggcagc gctcttgaac cagaactccg
      301 cgacagtcgc gtcataccaa cggccatccg gctcgtggta aagacccgag tcggcgttgc
      361 ccttgaccga gtaaccctcg ggagccgagc cgtccgccag cggcgccgca gcagcggccg
      421 gcaattcggc agcggcctca gccggagcct cggcgacctt ggccgccttg gtggccttcg
      481 gcgccggggc ggcctcggct gcgtcgacca gcgacgcggc cttctccgcc ttggccggct
      541 tctcagcctt ctcgaccttg gccggcttct cagccttgtc ggccttctcg accgttgctg
      601 ccacaggggc agtcgccgca gccttcgacg aggcagtcag cgtcgcgagc gcaccggccg
      661 tgaagaccac gtcgtcggcg cacagcacgt cgtaggtgtt gagctggtcg acagccagga
      721 cgtgaaccga gtcgaggttg cgcacgctct tgagaccgac ctggtcgcca cgctcaagca
      781 cgacgaggaa cttggtgcgg tcggtcagac cggccagcac cgccgccgcg gtcgaagtcg
      841 agggcgcgtc accggagacc agcacgtcga tgacgtggat ccggccgttg cgggcccggt
      901 ccgagagggc cccgcgcagg gcggcggcct tcatcttctt gggggtccgc tgcgagtagt
      961 cacgcggctg cgggccgtga acgacgccac cgccggcgaa ctggggcgca cgggtcgaac
     1021 cctgacgggc gcggccggtg cccttctggc ggtagggctt cttgccaccg ccggagacca
     1081 ggccccgggt cttggtggag tgcgtgccct gccgggcggc cgcgagctga gccgcgacga
     1141 cctggtggat cagcgggacg ttgacctgga catcgaagat gtcggccgga agtgcagtgg
     1201 tggtcgccat ggctgtcagg ctcccttcga cgcggagcga acgaggacga tcgcaccccg
     1261 ggggccggga acggcaccct tgatgagcag caggcccttg tcggcgtcga cggcctggat
     1321 cgcgaggttc tgggtggtct ggcgctcgcc gcccatccgg ccggccatcc ggacgccctt
     1381 gaagacgcga ccgggggtcg cgcaggcgcc gatcgaaccg ggcttgcggt ggttgcggtg
     1441 ggcaccgtgc gaggaggaga caccgtggaa gccgtgtcgc ttcatcacac cggcgaagcc
     1501 cttgcccttg gtcgttgcgg tggcgtcgac cagctgaccg gcctcgaagg tggagaccgt
     1561 gatctcctgc cccagggcgt agtcgctggc gtcggcggtg cgcagctcga ccaggtgccg
     1621 gcgcggggtg accccggcgc gctcgaagtg gcccttcatc ggcttgttga ccttgcgcgg
     1681 gtcgatctcg ccgtagccga tctgaacggc ggggtacccg tccgcgtcgc cgcggacctg
     1741 ggtgaccaca ttgggaccga ccttgatgac ggtcacggga accagccggt tatcggcgtc
     1801 ccacacctgg gtcatgccga gcttctcgcc cagcacaccc ttcactgtgc gtgtcgtcgc
     1861 gttgctcatc gtgatgtcct cagagcttga tctcgatatt gacatccgcc ggcagatcga
     1921 gacgcatcag cgagtcgacg gccttgggcg tcgggtcgat gatgtcgatg aggcgcttgt
     1981 gcgtgcgcat ctcgaagtgc tcgcgggagt ccttgtattt gtggggcgac cgaatcacga
     2041 cgaagacgtt cttctccgtc ggcagcggca ccggaccgac gaccgtcgca cccgcgcggg
     2101 tcaccgtgtc gacgatcttg cgtgccgaac tgtcgatgac ctcgtggtcg taggacttca
     2161 agcggatgcg gatcttctgt cccgccatcg tcgtaccgtc tctcttctcg ccaacacctg
     2221 cccttggtcg gccaggtcga acctccgacc cccgcggtcg ggcgtgtcgc gcccgctccg
     2281 cggcccgagc ctgggcacct aagtgccccg aacgatcggg tgtcttcagt ccggccgtag
     2341 ccggcgcctg cgcttccctc gggccaccag tggtgtccgt gccgccgcag acccgtagat
     2401 cgtgcgtccg gccgagaggc ggcggcatcg gctctggttc cacccagggc acgacgccgc
     2461 gcgtcaggca accggtctag tgtgccagat ggcgtggtga gcagccaaat cgccgaggat
     2521 ccacggtcac tcgcctcatc gactgacgag ttcgcccgcg acctgtcggc gccgcagggt
     2581 cgtcacagcg acccccgcga cgagcagcgc cccgcccacg agggccagca gaccgggccg
     2641 ctcgccgcgg aacaccaggg cggccgtcat gccgaccacg ggcaccagga gcgagtaggg
     2701 cgcgacctcg gcggcccggt gccgacccat gagggagttc cacacgccgt agccgaacag
     2761 cgaggccaac accgcggtgt atgccgtgga cagcaggctc ggcaccgaca ggtgggtcaa
     2821 tgcgtgcatg accgtctcgg gtccttcgac gagcaacgcg agggcgaaga gcgggatcgg
     2881 gacgaccagc cccgaccaca ccgtcagcga taggccgccc acgcccggca gacggcgcac
     2941 ggcgatgttg ccgcccgccc aggacgcggc ggccgccaca cacaacccga gcgctcccaa
     3001 cgggaccgat cccccgcgac ccaacgcaac gatccccaac cccagcagcc cgagcccgac
     3061 cccgatgacc tggcgacggc tgggccgctc ccgcaacagc gtcgcggcga acacgacggt
     3121 gaaggcggct tggatctgca ggaccagcga ggcgagccca ggcggcatac ccgcggacat
     3181 cgcgctgtag agcaacccga actgtccgac gctgagcagc agcccgacga gggcgatgtc
     3241 ctgccaccgc cctgcggggc gcggaacgaa gaagatcgcc ggcagcagca cgaggacgaa
     3301 gcgcatcgtc acgaagacca acggcggcac tccgggcaga ccccagtcga tgacgacgaa
     3361 gttgagcccc cagaccgtga cgacggcgag ggcgagcaga cgatcacggg gggtcacggg
     3421 tccaccctgt cgtgtctgac gtttcaggac aagcgcatat tcctgcacct gtatcggtag
     3481 tgtcgctaca tgatcgatct cgccgcagtg cggacgctcg atgccgtccg cgagcacggc
     3541 tccgtcgtcg cggccgccgc agcgctcggc tacaccccct cggcggtgtc ccagcagatc
     3601 aagcggctcg aacggcatac cggagtgcag ttgctcgaac gggtcggccg cggggtggcg
     3661 ctcaccgagt tgggccgccg ccttgccgac gacggcaacc gggtcctcgg cgacctcgag
     3721 gcgatcgagg cgcagctcgc ggcggcccac gggagtgctc ccctgcacgg tcaggtgcgg
     3781 atcgcggcct tctccacggg ggtgcgcggg ctggtcgccc ccgccctggc cacgttgcgc
     3841 cacaccgatc ccgggcttga cgtccgggtg gtcgagagcg acccgcacga ggcgctcgac
     3901 ctcgtcgccg ggggccaggt cgacaccgcg ctcgtgcaca actggggcga cctgccgctg
     3961 ccgatccccg cgcatgtcga ggacgtccac ctggggatcg acactgcgga tgtgctggtc
     4021 ccccgcgacc atcccttggc cactcgatcg gtcatcactg ccgcggacct ggacgggttg
     4081 gtgtggacct gcgccccggt cggcagcgtg tgtcacggct ggctcgtcca cttgcacgac
     4141 gcccatggcc tgcggcccga cgtgcgctac tgggcgatgg aattctcctc acagatcggc
     4201 ctggtcgaac acgggaacgc cgtcgccctg gtgccgcggt tgggccggga gtcgctgccg
     4261 tctggcgtca tcgccgtgcc ggtcgacgac ccggtgaccc ggcgcatcct gcaggtctgg
     4321 cgccggacga tggcgccgag cccggccatc cgccgggtcc acgacgtcct cacctcggcg
     4381 atggacgccg tggccgtcgc ccacccgcgc tgacgggggc gccgacagcg gcagcggcac
     4441 cggcgccggc accggcgccc gcgatcaagg cggcttcggc acccgcgatc aaggcggctt
     4501 cggtcagggc accggggcga ggaaggcagc gagcgccgcg gcatacatcg cttggtcggc
     4561 gacgccggcg agctcgcgga tgctgtgcat cgacaacatc ggcgcgccga ggtcgaccgt
     4621 cgtcaccccc acctggccgg ccgtgatcgg gccgatggtc gagccgcagg gcaggtcgct
     4681 gcgggtgacg aacacctgca acggcacatc cgcctgctcg cacgccaggt gcaacgccgc
     4741 tgcgcccacc gcgtcggtgg catagcgcag ctcggtctgg accttgagca cggggccgcc
     4801 gttcatctcg atccggtgcc cgggctcgtg cagctccgag tagttggggt gcgtggcgtg
     4861 ggccatgtcg gccgacgcga cgatcgtccc ggcgagcgcc cgccagaggt cgtcgcggcc
     4921 cccaccggcg gccaggacga tgcgttcgag gatcccgcgc agcatcggtg agtcggcgcc
     4981 gcgctcgctg cggctgccca cctcctcgtg gtcgaagagc acgatgaccg gccggtatgc
     5041 cgcgtcggcc tcctggtcgg ccgacgtgcc accggaggca gcgaccgcca acagggcgcg
     5101 gacggcggca tacgagctcg cgaggttgtc caagcgcggc gcggcgatga gctcctgggc
     5161 gcggccgatg cgggccgacg gctgggtgtc gtggagcatc gcgtcccagg cgagcacgtc
     5221 acccgggtcg gcgcccacct gctccccgag gtatgccgcg acgctcgctg gcgcctcccc
     5281 caccccccac aggggcacca ggtgttgttg cgggttgagg accagacccc gctcgttgac
     5341 gccgcggtcc agatggatcg cgagctgggc gacccgcagg aggggggccc ggtcgtggaa
     5401 cagccgggtc gacaccccag ttggggctga gttgtcgcgc acggcgaccc gtccggcgag
     5461 cccgaggtcg cggtcgagcc acgaattgat cagcagcccg ccatagggct cgacagccac
     5521 catctgccag ccggccttga ccaggtcggg gcgtggcttg acccgcagat tgggcgagtc
     5581 ggtgtgggtg ccgatgaccc ggaaaggtgc ggtgggcggc ataccccctg tctcgatcca
     5641 ggccacgagg gcgccgccgc gggcgatgta gtagcggcct ggctggctgg ggaacggcgc
     5701 cgactcgtcc agcgcggtga agccttcagc cgcgagcagc cggatcgcgg agtcgaccgc
     5761 gtggaagggc gtcggggagg catcgacgaa gtcgatcaac ccctgggccg tggagtcggg
     5821 gtcgaaggcg gtgtgcaggc tcacccatcg accttacccg cgctgccggt cagcagctcg
     5881 gtgggtctgt ccggcgtttg gccgccatgg cggtcaaact cgtgaagggc ggcctgatct
     5941 caccgccatc ggccgatgtg agcgccatgg cggccaaact cggagggctg agccgaggcc
     6001 ggggacgcgg cccgggaccc cacacgatgt gacgccgatg ggccccagcc gagtgg
//
LOCUS       DAORPW010000136         6040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3547_length_6040_cov_665.536675, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000136 DAORPW010000000
VERSION     DAORPW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3547_length_6040_cov_665.536675"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1565)
                     /locus_tag="PLB86_09995"
     CDS             complement(3..1565)
                     /locus_tag="PLB86_09995"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC65508.1"
                     /translation="MSINAFAALGVPASLADLLAKAGIDTPTPIQAATLPDSLAGRDL
                     LGRGRTGSGKTYAFLLPILARLAPNGVAPRNARTPRAPRALIVVPTRELAVQIEAALT
                     PLARLAGLNSLTIFGGVGQSPQVRALRAGVDIVIACPGRLEDLIGQGECRLDRVEVTV
                     LDEADHMSDLGFLPAVRRLLELTPASGQRLLFSATLDGAVTVIVKRYLRNPVTHEADS
                     PESATPDVEHHVLQVDSEQRMPVLVDLTSGSRRSVVFTRTKHRAKTLARKLNALGVSS
                     VELHGNLAQNARTRNLAAFHSGEASTLVATDIAARGIHVDDVELVIHADPPSEFKAYV
                     HRSGRTARAGAKGTVITLMEREQAAEVRALVRAAGIRPTTTSVGLGHPLLSELVPGER
                     RLVPVSARPAETVAAPEGRGSGTGGGGRGARSGRSGSGGSASPSAGSGRGGAARSGGT
                     RGSAPRSGAPRAGGSAPGAPRSDAGRSGAPRSGSARSGSAGGQRSGAARAGSLSAARM
                     SEQSGGRRRSGR"
     gene            complement(1757..>3633)
                     /locus_tag="PLB86_10000"
     CDS             complement(1757..>3633)
                     /locus_tag="PLB86_10000"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRC65509.1"
                     /translation="STEWPQFLAELAADGVLPADIVATHPTLHIAGIVGSIDNDMVGT
                     DMTIGTDSAMHRIVEALDAISSTAASHQRTFVVEVMGRRCGYLALTAAIAGGADFALI
                     PEAPPGDGWEEELCESLRRARERGRRESIVVVAEGARDRSGEPITADRICAVLEERLG
                     EQARVTILGHVQRGGSPSAFDRSMATMMGYDAVDQVLTAGPNDGAQLVGVHRNRVRRV
                     PLMRAVQENQAVARMVDEGHYEQALAARGDSYADLFGLLRTLSHPRTTLPADPKRIAV
                     LHAGGLAPGMNAAVRAVTLLGADAGHEILGVDGGFTGLCDGRLRTLTPRDVDGWAGLG
                     GAELGRRRGVLAPEQLYAVARAVDTHRIDALLVVGGLDGYESAQRIVRERGRYPSLDI
                     PVVCVPATIDNNLPGTELTVGADTALNAIVEAIDRIKQSALAAHRCFVIEVMGRECGY
                     LTLMSALSAGAERAYLPEDDLTLTDLAADVRSMTQRFRDGHGLFLAIRAEGAHPYYTT
                     DVLARIFEAEADDLYDVRQAVLGHVQQGGNPSPFDRVLAARLAADAVAAVSAALGAGR
                     SDAVAIGLDNGEIARTPLRRVDDLVDWARRRPRDQWWLRLRRVMRDLAEPAVGTRHTP
                     "
     assembly_gap    3634..3733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3802..4773)
                     /locus_tag="PLB86_10005"
     CDS             complement(3802..4773)
                     /locus_tag="PLB86_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HRC65510.1"
                     /translation="MEPEDWTVEDLVRTRHTEGSRPGERSDPCRLALLIEGGSSRSAY
                     SSGMVVAIEDLGLTTVFDAVYGVSGGALNGAWLLCGRAAQVMDTWWNREVMRGVIAPR
                     RALRGRPVVDTDYLVNTVYVDLVPMDFAAILGNSITFHPIATDAESGLAVDLAPLIHD
                     ESTLRLALRSSTGIPLLAGPPVHLAGRRFMDGGLAEMVPVRTALAQGATHAVVLRTRR
                     PGEDLRPPGNMQFHIIRRYLKRHAPGAIPAWRNRYAVAQDEEKLLATHPRVRQIRPPS
                     HGPTVPGTSRGARRLKATVDLGRATARSTLTRWLANSDPVGSTHSRG"
     gene            complement(4804..5457)
                     /locus_tag="PLB86_10010"
     CDS             complement(4804..5457)
                     /locus_tag="PLB86_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRC65511.1"
                     /translation="MTDQNLTHLYFLLDRSGSMASIRSETEAGFNAFIAEQRTAPGRC
                     LVTLAQFDDHYEEVYAARPVNEVPPLHLAPRGTTALLDSIGRLVTTAGERLAALPERD
                     RPGTVIVGIMTDGHENASHEWGFGAVRALIEQQTTIYSWDFLYMGADQDAIEVGAQLG
                     VSREQSLTYDRHRVGSAYASASGNISAMRAARAAGASPAAAREKLAFTEAQRADAEA"
     gene            complement(5474..5878)
                     /locus_tag="PLB86_10015"
     CDS             complement(5474..5878)
                     /locus_tag="PLB86_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65512.1"
                     /translation="MSTAPEPLAAAITAAQRSLADVDPGDPEQALPHLREAQLKVTEA
                     LDEAMAAAVLTEGSTIRQTATLAGLTENAVGPRLARTSLLAAYRDGDRVTRTGVERAR
                     YDLEEGRHKVSPPTPDEERPPLRFRARRPESR"
BASE COUNT          866 a         1924 c         2310 g          840 t
ORIGIN      
        1 ggtcagcgac cggagcggcg acggccgccg gactgctcac tcatgcgggc agccgagagg
       61 cttccggcgc gcgcggcgcc ggagcgctgc ccgcccgcgg atcccgagcg cgcggatccc
      121 gaacgcggag cgccggagcg tccggcgtcc gagcgcgggg cgcccggagc ggacccaccc
      181 gcacgcggcg cgcctgagcg gggggccgag ccacgagtgc caccggagcg agcagcgcca
      241 ccacggccgg acccggccga cggagaagcc gagccgcctg agcccgagcg acccgagcgt
      301 gcgccacgcc cgccaccgcc ggtgccacta ccgcggccct ccggtgcggc aacggtttcg
      361 gcgggtcgag cggagaccgg gactagccgg cgctcgcccg gcaccagctc ggacagcaac
      421 gggtgaccga gcccgaccga ggtcgtcgtc gggcggatcc cggcagccct gacgagggcg
      481 cggacctcgg cggcctgctc ccgctccatg agggtgatga ccgtgccctt ggcgccggcg
      541 cgcgcggtgc ggccggagcg gtggacatag gccttgaact cgctcggcgg gtcggcgtgg
      601 atgaccaact cgacgtcgtc gacgtggatc ccgcgcgcag cgatgtcggt ggccaccagc
      661 gtgctcgcct cgccggagtg gaaggccgcg aggttacggg tgcgggcgtt ctgggcgagg
      721 ttgccgtgca actcgaccga cgacactccg agcgcgttga gcttgcgggc gagggtcttg
      781 gcccggtgct tggtgcgcgt gaagacgacc gagcggcgcg aaccactggt cagatccacg
      841 agcactggca tccgctgctc ggagtcgacc tgcaacacgt ggtgctcgac gtccggagtc
      901 gccgactcgg gcgagtcggc ctcgtgggtg accgggttgc gcaggtagcg cttgacgatg
      961 acggtcactg ccccgtcgag ggtggccgag aagagcagcc gctgaccgct cgcaggggtg
     1021 agctcgagca ggcgtcgaac cgcgggaagg aagccgaggt cagacatgtg gtcggcctca
     1081 tcgaggacgg tcacctcgac ccggtccagg cggcactcgc cttggccgat gaggtcctcc
     1141 aaccgacccg ggcaagcgat gacaatgtcg acgccggcac gcagggcacg cacctggggc
     1201 gactggccga cgccgccgaa gatggtcagc gagttcaggc cggccaagcg agccagcggg
     1261 gtgagggcgg cctcgatctg gaccgccagc tctcgcgtgg ggacgacgat gagggcgcgg
     1321 ggtgcacggg gggtgcgggc gttgcgcgga gccactccgt tcggggcaag ccgcgcgagg
     1381 atcggcagga ggaaggcgta ggtcttcccc gatccggtgc ggccgcggcc gagcaggtcg
     1441 cgcccggcca acgagtcggg aagcgtggcc gcttggatgg gcgtcggggt gtcgataccg
     1501 gccttggcga gcaggtccgc cagcgaggcg ggcacgccga gagcagcaaa agcattgata
     1561 gacaaggaaa aatctccgtc gtggaaggtg ggaccgtctc gccggcgcac gtcgactcgt
     1621 ggacttctcc accagcggtg cggctcagat cgctacaacg tcaccaggca acccgaagcg
     1681 agactggccg ggctgctgcc tcaaccgtac ggcatacccg ccgggttccc cgcatcccgc
     1741 gttcgggccg ggtcgctcag ggggtatgcc gggtgcccac cgcgggctcg gcaaggtccc
     1801 gcatcacgcg ccgcagccgc aaccaccact ggtcgcgcgg gcggcgacgc gcccagtcga
     1861 cgaggtcgtc cacgcggcgc agcggagtgc gagcgatctc cccgttgtcc aggccgatcg
     1921 ccacggcatc cgagcgtcca gcgccgagtg ctgcagacac ggcggccacc gcgtcggcgg
     1981 ccaagcgcgc ggcgagcacc cggtcgaacg gtgaagggtt gccgccctgc tggacgtgcc
     2041 cgaggacggc ttggcgcacg tcatagaggt cgtcggcctc ggcctcgaag atccgggcca
     2101 gcacgtcggt cgtgtaatag ggatgggctc cctcggcgcg gatcgcgagg aacagcccgt
     2161 gcccgtcgcg gaaccgctgg gtcatcgacc ggacgtcggc ggcgaggtcc gtgagggtga
     2221 ggtcgtcctc cgggaggtag gcgcgctcgg cgccggccga cagggccgac atgagggtga
     2281 ggtagccgca ctcgcggccc atgacctcga tgacgaagca gcggtgggcc gccagggccg
     2341 actgcttgat ccggtcgatg gcctcgacga tcgcgttgag ggcggtgtcg gcgccgacgg
     2401 tcagctcggt gccggggagg ttgttgtcga tcgtggcggg cacgcagacg acggggatgt
     2461 ccagcgacgg atagcggccg cgttcgcgaa cgatccgttg ggcgctctca tacccgtcga
     2521 gtccgccgac gacgagcagc gcatcgatcc ggtgggtgtc aacggcgcgg gcgacggcat
     2581 acaactgctc gggggcgagc accccgcgac gcctgccgag ctcggccccg cccagccccg
     2641 cccagccgtc gacgtcgcgc ggcgtgaggg tgcgaaggcg accgtcgcac aggccggtga
     2701 acccgccgtc gacgccgagg atctcgtgac cggcgtcggc gccgagcagg gtgacggcgc
     2761 ggacggcggc attcatgccg ggggccagcc cgccggcatg caggacggcg atccgcttgg
     2821 ggtcggcggg cagggtcgtc cgggggtgcg acagggtgcg gagcaacccg aacaggtcgg
     2881 catagctgtc gccgcgggct gccagggctt gctcgtagtg accctcgtcg accatccgag
     2941 cgacggcctg gttctcctga acggctcgca tgagtggcac ccggcgcacc cgattgcggt
     3001 gcacgccaac gagttgcgcg ccgtcattgg gtccggcggt gagcacctgg tcgacggcgt
     3061 cgtagcccat catcgtggcc atcgaccggt cgaaggcgct ggggctgccg ccgcgttgga
     3121 cgtggccaag gatggtgact cgggcctgct cgccgaggcg ttcctcaagg acggcgcaga
     3181 tccggtcggc ggtgatcggc tcaccggagc ggtcccgcgc gccctcggcg acgacgacga
     3241 tcgactcgcg acgaccgcgt tcgcgggccc gtcgcaggct ctcgcagagc tcctcctccc
     3301 agccgtctcc ggggggtgct tccgggatga gggcgaagtc ggcgccaccg gcgatggcgg
     3361 ccgtgagcgc gaggtagccg cagcgtcgcc ccatcacctc gacgacgaag gtccgctggt
     3421 gactggcggc ggttgaggag atggcatcga gcgcctcgac gatgcggtgc atggcgctgt
     3481 cggtgccgat ggtcatgtcc gtgccgacca tgtcgttgtc gatcgagccg acgatcccgg
     3541 cgatgtgcag ggtcgggtgg gtggcgacga tgtcggcggg caagactccg tccgcggcga
     3601 gttcggcgag gaactgcggc cactcggtgg acannnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnntgggcgt cgccgccgct ggtgaggacc ccgacgcgtc gtgtgacagg
     3781 cgaaaccatg gagcccatcc ttcacccgcg ggagtgagtg gagccaaccg ggtcggaatt
     3841 ggcgagccag cgggtcaagg tggagcgggc cgtcgctcgg cccaggtcca ccgtggcctt
     3901 gagtcgtcgg gcgccacggg acgtcccggg cacggtgggg ccgtgcgagg gtggccggat
     3961 ctggcggacc cgagggtggg tggcgaggag cttctcctcg tcctgggcga cggcatacct
     4021 gttgcgccag gcggggattg cgccaggtgc gtggcgtttg aggtagcgac ggatgatgtg
     4081 gaactgcatg ttgcccgggg gccgcaggtc ctcgcccggc cgccgcgtgc gcaggacgac
     4141 ggcgtgggtc gcgccctggg cgagtgcggt gcgcacgggg accatctcgg ccaggccacc
     4201 gtccatgaac cgtctgcccg cgaggtgcac gggtgggccg gcgagcaggg gaatgccggt
     4261 cgaggatcgg agggccaggc gcagggtcga ctcgtcgtgg atgagggggg ccagatcgac
     4321 ggccaggccg ctctccgcgt cggtggcgat cgggtggaag gtgatcgaat tgccaagaat
     4381 ggcagcgaaa tccatcggca cgagatcgac atagacggtg ttgacgagat agtcggtgtc
     4441 aacgaccggc cgcccgcgca aggcccggcg tggggcgatg actccgcgca tgacctcgcg
     4501 gttccaccag gtgtccatga cctgggcggc gcggccgcac agcagccacg cgccgttgag
     4561 tgcgccgccg gagactccat agaccgcatc gaaaaccgtt gtgagaccaa ggtcttcgat
     4621 ggcgacgacc atgccggagg agtaggccga tcgggaactg ccgccttcga tgaggagggc
     4681 gagccggcac ggatcgcttc gttccccagg gcggctgccc tcggtatgcc gcgtccgcac
     4741 gaggtcctcg accgtccagt cctcaggctc caccgttgga gcctacgcgg atcgcggcgg
     4801 cggtcaggcc tcggcgtcgg cgcgttgggc ctcggtgaag gcgagcttct cgcgggcagc
     4861 ggcggggctg gcgcccgcgg cgcgggctgc gcgcatggcc gagatgttgc ccgaggccga
     4921 cgcgtatgcc gatccgaccc ggtggcggtc gtaggtgagc gactgctccc ttgagacacc
     4981 cagttgagcc ccaacctcga tcgcgtcctg gtcggccccc atgtagagga agtcccagga
     5041 gtagatcgtg gtctgctgtt cgatgagtgc tcggacggcg ccgaatcccc attcgtgaga
     5101 ggcgttttcg tgaccgtcgg tcatgattcc gacgatgacc gtccccgggc ggtcgcgctc
     5161 gggaagggcg gcgagccgct cgcccgcagt cgtcacaagg cggccgatgg agtcgagcag
     5221 cgcggtggtg ccgcgggggg cgaggtgcag gggcgggacc tcattcacgg ggcgcgcggc
     5281 atacacctcc tcgtagtggt catcgaactg cgcaagggtc accaggcaac ggcccggcgc
     5341 ggtgcgctgc tcggcgatga acgcgttgaa gccggcctcc gtctcggacc ggatcgaggc
     5401 catcgagccg gaccggtcga gcaggaagta gaggtgggtg aggttctgat cggtcatcgg
     5461 atctccttgt ggatcaacgg gattcggggc gacgcgcgcg gaatcgcagc ggcggtcgct
     5521 cctcgtccgg ggtgggcggg gacaccttgt ggcgcccctc ttcgaggtcg tagcgcgcgc
     5581 gctccacgcc cgtgcgggtc acccgatcgc cgtcccggta tgccgcgagc aggctggtgc
     5641 gcgccaggcg cgggccgacg gcgttctcgg tgaggccggc gagggtggcg gtctgacgga
     5701 tcgtcgaccc ctcggtgagc accgcggcgg ccatcgcctc gtcgagcgcc tcggtgacct
     5761 tgagttgggc ctcgcgcagg tgcgggaggg cctgctccgg gtcgccaggg tcgacatcgg
     5821 ccaacgaccg ctgggcggcg gtaatggctg cggcgagtgg ctcaggtgcg gtgctcatga
     5881 tgtgatggta acgcagtaac tgcggtgccg caactggagc catgaggtga cgatgccccg
     5941 gtccgccgcg cgggcggcat accggggcat cgtcggctgt gggtcgcgct cagtgctgag
     6001 cgtcctgcgg gctcggtgcg gtggtgccgt aggggtgctg
//
LOCUS       DAORPW010000137         6010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3573_length_6010_cov_854.636608, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000137 DAORPW010000000
VERSION     DAORPW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3573_length_6010_cov_854.636608"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..640)
                     /locus_tag="PLB86_10020"
     CDS             complement(128..640)
                     /locus_tag="PLB86_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65513.1"
                     /translation="MDLGNLLGGIDTDAIADVVKVVVDNRAMIEQLGRLPELFGQFAD
                     NLQGAGQEAGSAALALVGADGTSGARGALASAATALAGIVTTLDSGIARIAETAEGAA
                     KVPLMGGPAGHLAEAAKDMGESTAQLGTLAASMGSIAEVLDEVASALRNVGVYLTDSG
                     TQARGFVGTS"
     gene            complement(707..>1954)
                     /locus_tag="PLB86_10025"
     CDS             complement(707..>1954)
                     /locus_tag="PLB86_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC65514.1"
                     /translation="AVPTSAMLTGDEVAAVVADARPSLVIAAPEHAATVPHGIPLVGP
                     EHLAKWRADLAPAPWRDSRADDPAFLVYTSGTSGEPKGVLHAHRSAWGRRPMYAGWEG
                     LRPGDVMLHAGAFNWTYTLGVGLTDPWANGGTAVVYNGPRDPAVWGRLVKRCGATIFA
                     AVPGVYRQLLASGALAPADLATLRHGLTAGEALSREMYAAWQEATGLPLYEALGMSEI
                     STYVSSGPTVPTRPGSPGKPQEGRCIAILPVEPTDPAEGDAPLPTGHTGLLAIHRTDP
                     GLMLGYWERPDEDAATRRGEWFVGGDLAHLDDDGYLIHHGRADDVMNPGGFRVSPQEV
                     EHVLAAAPGVADVGVTEVEIRAGVRVVGAFVVARPQGCDPEAVRAYAAEHLAAYKVPR
                     VVRLVSTLPRTANGKLVRRELSS"
     assembly_gap    1955..2054
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2055..3226)
                     /locus_tag="PLB86_10030"
     CDS             complement(<2055..3226)
                     /locus_tag="PLB86_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRC65515.1"
                     /translation="MSNESGSEGTPAPRGHARVPGDSDRRPTWVALAQSKRVLWVLVA
                     AGLALLIGVSVFQALGSFLFLLLLAWLLSIAMEPMVGWLIRRGMSRAMASAAVLFGLM
                     AVTVGIAAMFGQVFISQAQELKDAFPDAVRQAVAWVNTTFNTSFNTDQIYTALQLSPE
                     RISEFAQRFGGGLLGVFGAVVTFIFNLVTILVFAYYLSADSIKLRRTIGSYLPPRYQP
                     MLMTIWTIAIDKTGGYVVSKVILSTFSSVAYALFFLILDVPFWLPLGVLMGISGQFIP
                     IIGTYIGVVVPALFSLLRDPLDAVWIVVFATIYQQFESYWLTPKISNRTMDVHPAVAL
                     GSVFVGVALMGPIGAVIGIPVAAAVITIADTFRRRHELLPELETLQDDDGGSDGDSA"
     gene            complement(3437..3622)
                     /locus_tag="PLB86_10035"
     CDS             complement(3437..3622)
                     /locus_tag="PLB86_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HRC65516.1"
                     /translation="MLGAGEERDPLLTPGEVAGLFRVDPKTVTRWAKAGKLTAIRTLG
                     GHRRYRSSEVHRLLGGQ"
     gene            3892..4131
                     /locus_tag="PLB86_10040"
     CDS             3892..4131
                     /locus_tag="PLB86_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009213040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HRC65517.1"
                     /translation="MGRGRAKAKQTKVARELKYFSPDTDLSALERELHSATHRGGQSF
                     NQTDDVDDDEDDEEEDDDADDGEVDDDYASWAAGR"
     gene            complement(4245..4784)
                     /locus_tag="PLB86_10045"
     CDS             complement(4245..4784)
                     /locus_tag="PLB86_10045"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC65518.1"
                     /translation="MSDATPPAGGMDPALDPPAVATVGTERELLQAWLDFHRQTLRWK
                     CSGLTPEQLCEASAPPSRMSLAGLMRHMTDVEVWWFGRVAVGDESATDRYPGQDEDLC
                     EATAPTVAADWEAFEAAIRRSDEVFATLNLDATAAWMHPRRGPVSNRWILIHMVEEWA
                     RHNGHADLIRERIDGAAGD"
     gene            complement(4820..5923)
                     /gene="purM"
                     /locus_tag="PLB86_10050"
     CDS             complement(4820..5923)
                     /gene="purM"
                     /locus_tag="PLB86_10050"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRC65519.1"
                     /translation="MTYAAAGVDVEAGDKAVELMKESIRRATRPEVLGGLGGFAGLFD
                     ASALARMTRPILATSTDGVGTKVAIAQALDRHDTIGFDLVGMVVDDIVVCGAEPLFMT
                     DYIATGKVVPERIAAIVGGIARACEQAGVALVGGETAEHPGLLDPDEYDVAGAATGVV
                     EYAGILGPQRVEAGDVVLALASSGLHSNGYSLVRRVIAAAGWSLDRHVEEFGHTLGEE
                     LLTPTRVYAADLLALIRTPGIDIHALSHVTGGGLAANLARVLPRGSYARVDRSTWTPP
                     AVFEVIGALGRVPRSDLERTLNLGIGFVAVLPAAHAAAAEQVCRARGIPAWVLGEVGE
                     EATWTPQPDVEVTRGAKGVDGGAVQVVGEHPKP"
BASE COUNT          943 a         2044 c         2086 g          837 t
ORIGIN      
        1 gtgaggtgga tgatcccctc gtcgacggtg aacttgtcct ccagcgacag cggtggaagc
       61 agggtgctgc tcatctcggc atcctatgtc cgcggcatac cggcaaccgg tgctgcgaac
      121 gcgcggatca ggacgttccg acgaagcccc tggcctgagt gccgctgtcc gtgaggtaga
      181 caccgacgtt ccgcaaggca gaagcgacct cgtcgagcac ctcggcgatc gaccccatcg
      241 aggccgccag cgtgcccaac tgcgccgtcg actcgcccat gtccttggcg gcctccgcga
      301 ggtgccccgc cgggccaccc atgagcggca ccttggcggc cccctcggcg gtttcggcga
      361 tccgcgcaat gccactgtcc agcgtggtga cgatgccggc gagcgccgtc gcggccgacg
      421 ccagcgcgcc gcgggcgccg gacgtgccgt cggcgccgac cagcgcgagc gcagcggatc
      481 cggcctcctg gccggccccc tggaggttgt cggcgaactg cccgaacaac tccggcaggc
      541 ggcccagttg ctcaatcatc gcgcggttgt cgacgaccac cttgacgacg tcggcgatgg
      601 cgtcggtgtc gatcccgccc agcaggttac ccaggtccat gcgcgcatcc tagggccgcc
      661 ggcgcggccg gttctcggcc cagaactgcg cgggacccag cggaagtcac gagctcaatt
      721 ctcggcgcac cagtttgccg ttggcggtcc ggggtagcgt cgagaccagt cggaccaccc
      781 gcgggacctt gtatgccgcg aggtgctccg cggcatacgc ccggaccgcc tcggggtcgc
      841 acccctgtgg gcgcgcgacg acgaacgcgc cgacgacccg gaccccggcc cggatctcga
      901 cctcggtgac cccgacgtcg gccacgccgg gtgcggcggc gaggacgtgc tcgacctcct
      961 ggggcgagac ccggaatccg ccggggttca tgacatcgtc ggcccgcccg tggtggatca
     1021 ggtagccgtc gtcgtccagg tgggccaggt cgccgccgac gaaccactca ccgcggcggg
     1081 tggcggcatc ctcgtcgggc cgctcccaat agccgagcat gaggccgggg tcggtgcgat
     1141 ggatcgccag caacccggtg tggcccgtcg gcagcggcgc gtcgccttca gccggatccg
     1201 tcggctcgac cggcaggatg gcgatgcagc ggccttcctg cggcttgccc ggcgagccgg
     1261 gccgggtcgg cacggtcggc cccgacgaga cgtaggtgga gatctcgctc atccccagcg
     1321 cctcgtagag cggcagcccg gtggcctcct gccacgcggc atacatctcg cgcgagagcg
     1381 cctcgccggc ggtgaggccg tgccgcaggg tggccagatc ggccggtgcg agcgccccgc
     1441 tcgcgagcag ttgccgatag acgcccggaa ctgccgcgaa gatcgttgcg ccacaacgct
     1501 tcacgagcct cccccagacg gccgggtcgc gggggccgtt gtagacgacc gccgtcccgc
     1561 cgttggccca cgggtcggtc agcccgacgc cgagcgtgta ggtccagttg aacgcacccg
     1621 cgtggagcat gacgtcaccc ggtcgcagac cctcccaccc ggcatacatg ggccgccgtc
     1681 cccacgcgga gcggtgggcg tgcaggacgc ccttcggctc gcccgaggtc cccgaggtgt
     1741 agacgaggaa ggccgggtcg tcggctcggc tgtcgcgcca cggagcgggg gcgaggtcgg
     1801 cgcgccactt cgcgaggtgt tccggaccga caaggggtat gccgtgcggc acggtcgctg
     1861 cgtgctccgg cgccgcgatc accagggaag ggcgggcgtc ggcgaccacc gcggccacct
     1921 cgtcgccggt gagcatcgcc gaggtcggca cggcnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnngcagag tcaccgtcgg agcctccgtc gtcgtcctgc agggtctcca
     2101 actcggggag caactcgtgg cgccgtcgga acgtgtcggc aatggtgatc accgccgcgg
     2161 cgacgggaat gccgatgacg gccccgatcg gccccatgag ggccacgccg acgaacaccg
     2221 acccgagggc caccgctggg tggacgtcca tcgtccggtt gctgatcttg ggggtcagcc
     2281 agtagctctc gaactgctgg tagatcgtgg cgaacacgac gatccacacg gcgtcgagcg
     2341 ggtcgcgaag gagcgagaac aaggcgggga cgacgacacc gatgtaggtg ccaatgatcg
     2401 ggatgaactg gccggagatc cccatgagca ccccaagggg cagccagaag ggcacgtcaa
     2461 ggatgaggaa gaacagcgcg tacgcgactg aggagaaggt cgacaggatg accttcgaga
     2521 cgacgtaacc gccggtcttg tcgatcgcga tggtccagat ggtcatgagc atcggctggt
     2581 accggggcgg caggtaggag ccgatcgtgc ggcgcagttt gatgctgtcg gccgacaggt
     2641 agtaagcgaa gacgaggatc gtcacgaggt tgaagatgaa cgttacgacc gcaccgaaga
     2701 caccgagcag gccgccgccg aagcgctggg cgaactcgga gatccgctcg ggcgagagtt
     2761 ggagggcggt gtagatctgg tcggtgttga acgacgtgtt gaacgtggtg ttgacccacg
     2821 cgacggcttg gcggacggcg tcagggaagg cgtccttgag ctcctgcgcc tgtgagatga
     2881 agacctgacc gaacatggcc gcgatgccga cggtgacggc catcagcccg aacaggacgg
     2941 cggctgacgc catggcccga ctcatgccgc ggcggatgag ccacccgacc atcggctcca
     3001 tggcgatcga cagcagccag gccagcagca ggaggaacag gaagctgccc agcgcctgga
     3061 agaccgacac gccgatgagc agggcgagcc ccgcggccac gaggacccag aggactcgct
     3121 tggactgcgc gagcgcgacc caggtcggcc gacgatcgct gtctcccggc accctcgcgt
     3181 gcccccgggg ggccggggtg ccctcagaac cgctctcatt gctcacagaa accactgtag
     3241 ttgggccgcg gcgcaggggg cagagatggc gtccggtatg ccgcgagctg gttcagggca
     3301 gcacgaatcg cagaacgtcg ttaacccgca ttatgcggcg aacgttctgt ttgctctagc
     3361 cgtgcggcgc cttcccccga aggcactccg atcgctccgc acccccacga tgcgtcacga
     3421 ccgcgttccg cttggcctac tgcccgccga gcagcctgtg cacttccgag ctgcggtagc
     3481 gccggtgtcc gcccaatgtg cggatcgccg tcaacttgcc cgccttagcc catcgggtga
     3541 ccgtcttcgg gtccactcgg aagagccctg ccacttcacc aggcgtgagc aaggggtctc
     3601 gctcctcgcc cgctcccaac atcattcttt ggtactccat tcggatgacc ccgcgtcaat
     3661 cccctgcgcg ggacactctc gcacgaccga ggcacctctg gtgggaaacg ctcccaactg
     3721 atcaccagga aggacccgtc agacagaacc gccaacgtca ggtagcgttg tccccacgga
     3781 gacacacatt tcccggggcc gctccgcggc gacccgccgt gcactgccga tcggcacgtg
     3841 accggcgggc ctggcgatgc cgccccggca gacgcatgag ggggtcacgc catggggcgc
     3901 ggccgggcca aggccaagca aactaaggtc gcccgggagt tgaagtattt ctctcccgac
     3961 accgatctca gcgcgcttga gcgcgaacta cacagcgcca cccaccgtgg cggccagtcg
     4021 ttcaaccaga ccgacgacgt cgatgatgac gaagacgacg aggaagagga cgacgacgct
     4081 gatgacggtg aggtggacga cgactacgcg tcgtgggcgg ctggtcgctg aagcgaccgg
     4141 ccctcacccg tagcggcacc tcatgcggtc ctagaccgtg agttgctgac acccgaccgc
     4201 cggtcgggcc caaggcatgc cctcaagcgc cggggactgg cgactcagtc gccggccgcg
     4261 ccatcgatcc gttcccggat gaggtcggca tggccgttgt gccgcgccca ctcctcgacc
     4321 atgtggatga ggatccaccg gttgctcacc ggcccgcgcc tggggtgcat ccacgcggct
     4381 gtcgcgtcca ggttgagcgt ggcgaacacc tcatccgacc gacggatggc tgcctcgaac
     4441 gcctcccagt cggctgccac ggtcggtgcc gtcgcctcgc agagatcttc gtcctggccg
     4501 ggatagcgat cggttgccga ctcgtccccc accgcgaccc gtccgaacca ccagacctcg
     4561 acgtcggtca tgtgccgcat cagcccggcc agactcatcc gcgagggcgg cgcgctggcc
     4621 tcgcacagct gctccggcgt caaccctgag cacttccagc gcagcgtctg acgatggaag
     4681 tcgagccagg cctgcagcaa ctcgcgctcg gtgccgacgg tcgccactgc gggcgggtcg
     4741 agggcggggt ccatgccgcc tgcgggcggg gtggcgtcgc tcatcgcccc accgtaggcc
     4801 agtgccgggc gcccgcgcct cagggtttcg ggtgctcccc gacgacctgc acggctcccc
     4861 cgtcgacgcc cttcgcccca cgggtcacct cgacgtccgg ctgcggcgtc cacgtcgcct
     4921 cctcgccgac ctcccccagg acccacgccg gtatgccgcg cgcgcggcat acctgctcgg
     4981 cggcggctgc gtgcgcggcg gggaggacgg ccacgaagcc gatgccgagg ttgagggtgc
     5041 gctcaaggtc gctgcggggc acccggccga gtgcgccgat gacctcgaag accgccggcg
     5101 gggtccaggt cgaccggtcg acccgggcgt agctgccgcg gggcaggacg cgggcgaggt
     5161 tggcggccag gccaccgccg gtgacgtgcg agagcgcgtg gatgtcgatg ccgggggtgc
     5221 ggatgagcgc gaggaggtcg gcggcataca cccgcgtggg ggtgagaagc tcctcaccca
     5281 gcgtgtgccc gaactcctcg acgtgccggt ccagcgacca gccggccgcc gcaatgaccc
     5341 ggcggaccag ggagtagccg ttggagtgca ggcccgagga cgcgagcgcg aggacgacat
     5401 cgccggcctc gacgcgctgc gggccgagaa taccggcgta ttcgacaacc ccggtcgcgg
     5461 cccccgcgac gtcgtactcg tcggggtcca gaagccccgg gtgctcggcc gtctcgccgc
     5521 cgacgagggc gaccccggcc tgctcgcagg cgcgggcgat gccgccgacg atggccgcga
     5581 tccgttcggg gacgaccttg ccggtggcga tgtagtcagt catgaagagg ggctcggcgc
     5641 cacagacgac gatgtcgtcg acgaccatgc cgaccaggtc gaagccgatg gtgtcgtgcc
     5701 gatcgagggc ctgggcgatg gcgaccttgg tgccgacccc gtcggtcgac gtggcgagga
     5761 tcgggcgggt catccgggcg agggcgctgg cgtcgaagag cccagcgaaa ccgccgaggc
     5821 cgccgagcac ctcgggtcgg gtggcccggc ggatcgactc cttcattaac tcgacggcct
     5881 tgtcgcccgc ttcgacgtcg actcctgccg cggcatacgt gatggggccc ggctccgagg
     5941 tcgaggtcga ggtcgaggtc gaggaggctg gcggcggggt cgacggagca gtcacgtgct
     6001 tagcctaggg
//
LOCUS       DAORPW010000138         5950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3625_length_5950_cov_635.971671, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000138 DAORPW010000000
VERSION     DAORPW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3625_length_5950_cov_635.971671"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PLB86_10055"
     CDS             complement(<1..530)
                     /locus_tag="PLB86_10055"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRC65520.1"
                     /translation="MSVATGLQRHRRSLRQFVKFGIVGGSGVLVNMLVTIVMHKLHGG
                     TANGRDPLFAIPGTPYFARYLHLVWIGAFLVANVWNFQLNRGWTFRASGHSGWWRQFW
                     PFLAVGSAAAVVGLFVITALTHPQSPIYLPDPYFSESAGLRSRFYWAQLITIVVTMPI
                     NFVANKLWTFRAVRHHP"
     gene            complement(604..1656)
                     /locus_tag="PLB86_10060"
     CDS             complement(604..1656)
                     /locus_tag="PLB86_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRC65521.1"
                     /translation="MQRIMARAARSALLMGLACAGLPFFGALLWLSTRPDLIDEWLAQ
                     PLGIRALHLAGLLLAMMLIGGTVATVVAGRATESIQAPIDELSRRAEQVGGGETQVPF
                     TPTGVAEIDLVSDALSRSAQRLSRALATERSFASDASHQLRTPLTALMMRIEEIAATD
                     DLAVAHEEAAVAIAQVDRMSRVVDDLLQRRRSDPEGQLPEVSLDTVIATLQREWQPAF
                     ETARRSVRVHGERGLVVRATPIALSQVLSTLLENSLVHGRGTVDVHARRSGPSAVVEV
                     RDEGDGIPAALAPHIFERSVSSGAGSSTGLGLALARDLAESCGGRLDLVARTPATFAL
                     FLSEARDGYQPSASGR"
     gene            complement(1663..2355)
                     /locus_tag="PLB86_10065"
     CDS             complement(1663..2355)
                     /locus_tag="PLB86_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC65522.1"
                     /translation="MTRVLLVEDDPGISEPLARALRREGYDVDVREDGRAALEGARER
                     PDLVVLDLGLPHLDGLEVCRRMRADGRTMPVLILTARADEVDTVVGLDAGADDYVTKP
                     FRLAELLARVRALLRRTPDGNPVSDTEDLRIDPEGRRAWFQGEELQLTAKEFDLLRVL
                     LREQGRVVTREQLMKEIWDTTWYGSTKTLDMHISVLRRKLGDDVAQPRHISTVRGLGF
                     RFERPESASEPS"
     gene            complement(2434..3216)
                     /locus_tag="PLB86_10070"
     CDS             complement(2434..3216)
                     /locus_tag="PLB86_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC65523.1"
                     /translation="MLVRIERHGDGGHVAELVLDRPEAMNAVSTAMAEAIAAATSALA
                     ADPSVRCVVLTSSHPKAFCVGADLKERNTMTDADLMAQRPLARAAYGGVLALPMPAVA
                     AVDGFAFGGGLELALSCDLLVAGDGAIVGLPEVSVGVIPGGGGTQLLARRIGWSRAAR
                     LIFTAVKLPAAEAATFGVIDELVPAGTARERALAIATQIAANSPVGLRSAKRAMRLGL
                     DVDLASGLEIEDACWRATAFSGDRAEGVAAFAEKRAPRWPGR"
     gene            complement(3279..4373)
                     /locus_tag="PLB86_10075"
     CDS             complement(3279..4373)
                     /locus_tag="PLB86_10075"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRC65524.1"
                     /translation="MTVGPAAHNEGMLPSTAAAARTADSLNSDDVASLLVGTSREFSR
                     SDVSRLAGVSVVGARKFWHALGFASTTSSDRMFTEADLRALRRMNMLVRSSVIDEATA
                     LALTRAFARNTDRLAAWQVQLISEAMTASSGVADVDGPLNEAIAQRTARLIVDLADEL
                     EPMLVYAWRRHLADAVSRMLADVGSGSESDAPGQLRCVGFADLVSFTTLVRQLSERDL
                     ARTVQRFEALATDVVTAHGGRVVKTVGDEVLFSTRTPAPGAAIALDLVDALAEDEVLP
                     DLRVGVSFGTVVSRLGDVFGTTVNRAARLTALAHPNAVLVDGPLATALTSQSGFRLTA
                     MRRRTLRGVGSVTPHVLARTSTPTRRGRYE"
     gene            complement(4381..5214)
                     /locus_tag="PLB86_10080"
     CDS             complement(4381..5214)
                     /locus_tag="PLB86_10080"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRC65525.1"
                     /translation="MAHPMGNRSDLDVARLTSRLCRPPRTWASVDVFDELMSTNALAM
                     VRGQVWAVVVAEHQFSGRGRLDRRWFAPAFTALTFSVSVPVPPGATGWVPLVAGLAVA
                     EAVLTVTGLPAELKWPNDVLLPADDHRKVAGILCEYRPPADGADELIVIGVGLNVSQT
                     RDQLPVDTGTSLTLAGAEPVDREDLLVTVLDRIAVRYAALQAGGAAADGVRAAYRAAC
                     STLGTEVRLERPGAGTSTAHALDIDADGALVVDEGSGPTRYAAGDVTHVRPASGGGGL
                     A"
     gene            5238..>5950
                     /locus_tag="PLB86_10085"
     CDS             5238..>5950
                     /locus_tag="PLB86_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRC65526.1"
                     /translation="MSNAGVPDAAAPTLDLSTTAGKLAELRRRVAEVANAGSTTAVEK
                     QHAKGKKTARERIAMLLDEGTFIEMDKYARHRSNQFGQEKHRPYGDGVVTGYGQVDGR
                     QIAVFAQDFTVFGGSLGEVFGEKIVKVMDWAAKIGVPVVGLNDSGGARIQEGVVSLGL
                     YGEIFFRNVRSSGVVPQISVIMGPCAGGAVYSPAITDFTVMVDKTSHMFITGPDVIKT
                     VTGEEVEFEDLGGARAHNT"
BASE COUNT          978 a         2070 c         2068 g          834 t
ORIGIN      
        1 gggtggtgcc ggacggcacg gaaggtccac aacttgttgg cgacgaagtt gatgggcatg
       61 gtcacgacga tggtgatcaa ctgggcccag taaaaccgcg agcgcagacc ggccgactcg
      121 ctgaagtacg ggtcgggcag gtagatcggt gactgcgggt gggtgagcgc cgtgatgaca
      181 aacaggccga caaccgcggc ggcgctgcct acggcgagga acggccagaa ctgacgccac
      241 cagccagagt gcccggaggc ccggaacgtc cagccacggt tgagctggaa gttccagacg
      301 ttggcgacga ggaaggcccc gatccacacc agatgcaggt agcgggcgaa gtagggcgtc
      361 ccggggatag cgaacaacgg atcccgcccg ttggccgtgc ccccgtggag tttgtgcatg
      421 acgatggtca ccaacatgtt gaccaggacg cccgagccgc cgacgatgcc gaacttgacg
      481 aactggcgaa ggctgcgccg gtgtcgctgc agtcccgtcg ctacgctcac ggtctcggag
      541 cctaccctcc ggcccggtga ccgcctcccg agcgaccgct ccctgggcgg cccaccaact
      601 ccgtcaccga ccgctcgccg acggttgata cccgtcgcgg gcctcggaga ggaacaacgc
      661 gaaggtggcg ggcgtccggg cgacgaggtc tagccgcccg ccacacgact cggccaggtc
      721 gcgcgccaac gccaacccga gcccggtgct tgatccagct ccgctgctca ccgaccgctc
      781 gaagatgtgc ggcgcaagag cagcgggtat gccgtctccc tcgtcgcgca cctcgaccac
      841 cgcggacgga ccgctgcgtc gagcatgcac gtcgacggtg ccccgtccgt ggaccaagga
      901 gttctccagc agcgtcgaga ggacttggct caaggcgatc ggggtcgctc gcacgaccag
      961 gccgcgctcg ccgtggacgc ggacactgcg tcgagcggtc tcgaacgcgg gctgccactc
     1021 gcgttgcagg gtcgcgatga cggtgtcgag cgacacctcc ggcaactgcc cttccgggtc
     1081 gctgcgccgc cgctgcaaca ggtcgtcgac gacccggctc atccggtcga cctgggcgat
     1141 ggcgacggcg gcctcctcgt gggccaccgc gaggtcgtcg gtcgcggcga tctcctcgat
     1201 ccgcatcatc agtgcggtga gcggggtgcg cagctggtgc gaggcgtcgg aggcgaagct
     1261 gcgctcggtg gccaacgccc gagagagccg ctgcgctgat cgggacaggg catcggagac
     1321 gaggtcaatc tcggcgaccc ccgtcggcgt gaacggcacc tgggtctccc cgccgccgac
     1381 ctgctcggcc ctgcggctca gctcgtcgat cggggcctgg atcgactcgg tcgcgcggcc
     1441 ggcgaccacc gtcgcgaccg tccctccgat gagcatcatc gcgagcagga gcccggctag
     1501 gtgcagggcg cggatgccga gcggttgggc caaccactcg tcaatcaggt cggggcgggt
     1561 tgacagccac agcagggctc cgaaaaacgg caggcccgcg caggccaaac ccatgagcag
     1621 ggcggatcgg gccgcgcgcg ccatgatccg ctgcatgaca ggtcagctcg gctcgctggc
     1681 gctctcggga cgttcgaagc ggaacccgag tccgcgcacc gtcgagatgt gccgaggttg
     1741 ggccacgtcg tcgccgagct tccgtcgcaa tactgagatg tgcatgtcga gggtcttggt
     1801 cgacccgtac cacgtggtgt cccagatctc cttcatgagc tgttcgcggg tgaccacccg
     1861 gccctgctca cgaagcagca cccgcaacag gtcgaattcc tttgcggtca gctgaagttc
     1921 ctcaccctgg aaccacgcgc gccgaccctc ggggtcgata cgaaggtcct ccgtgtcgga
     1981 gacggggttg ccgtcagggg tccgccgcag cagggcgcgg acgcgagcga gcagctcggc
     2041 caggcggaac ggcttggtga cgtagtcgtc agcgccggcg tcgagcccga cgacggtgtc
     2101 gacctcatcg gcccgcgccg tgaggatgag caccggcatg gtgcggccgt cagcccgcat
     2161 gcggcggcat acctccaggc cgtccaggtg cgggaggccg aggtccagga caaccagatc
     2221 aggacgttct cgggcgcctt cgagggcagc ccggccgtct tcacgcacat cgacgtcgta
     2281 cccctcacgt cgcagcgcgc gcgccagggg ctcagagatg cctgggtcgt cctcgacgag
     2341 cagcacccgg gtcatgggcc agatggtatg ccttcggacc tcacgccgca tgtacggccg
     2401 gtgcggcccg acctcctatg agcgcaattc gggtcagcga ccgggccacc ggggagcccg
     2461 cttctcggcg aaggcggcaa ccccttcggc acggtcaccg ctgaaggccg tagcccgcca
     2521 gcaggcgtcc tcgatctcca ggccggaagc caggtcgaca tcaaggccca ggcgcatggc
     2581 tcgcttggcc gagcgcaacc ccaccggtga gttggccgcg atctgggtgg cgattgccag
     2641 ggcccgctcc cgagccgtcc cggccggcac gagctcgtcg atgaccccga acgtcgcggc
     2701 ctcggcagcc gggagtttga ccgcggtgaa gatcaaccgg gccgctcggg accagccgat
     2761 ccgacgcgcg agcagttggg tgccgccgcc tccggggatc actccgacag acacctcagg
     2821 caggccgacg atcgcgccgt caccggcgac gaggaggtcg caggagaggg cgagctccag
     2881 cccgccgccg aaggcgaacc cgtcgacggc cgccaccgcg ggcatcggca gggcaagaac
     2941 cccaccgtat gccgcccgcg ccaggggacg ctgggccatg aggtcggcgt cggtcatcgt
     3001 gttgcgttcc ttgaggtcgg cccccacgca gaacgccttc gggtgcgagg aggtcaggac
     3061 gacgcagcgc acgctcgggt cggccgcgag ggcactcgtc gctgcggcga tcgcctcggc
     3121 catggccgtc gagaccgcgt tcatcgcttc aggccgatcc aggacgagct cggcgacgtg
     3181 gccgccgtcg ccgtgccgtt cgatgcgcac caacaggcgc ggttcttgcg gacagacggt
     3241 cgtgccgatg ggctgggggt ccgggccggc gctgctggtc actcgtatct ccctcgtcgg
     3301 gtcggggtgc tcgtccgagc gagcacgtgg ggggtcacgg agcccacgcc gcgcagcgtt
     3361 cgacgccgca tcgcggtgag ccggaagccg gactgactgg tgagagccgt cgccagcggt
     3421 ccatcgacga ggacggcgtt gggatgggcg agggcggtca gccgggcggc ccggttgacg
     3481 gtcgtcccga agacgtcgcc caaccgggac acgacagtgc cgaacgagac cccgacccgc
     3541 aggtccggca gcacctcatc ctcggcgagc gcgtcgacca ggtcaagggc gatggccgcc
     3601 ccgggcgcag gggtgcgcgt cgagaagagc acctcgtcgc cgaccgtctt gaccacccgt
     3661 ccgccgtgcg cggtgaccac atcagtggcc agggcctcga accgctggac ggtccgagcg
     3721 aggtcgcgtt cggacagttg gcgcaccagc gtcgtgaacg acacgagatc ggcgaaaccc
     3781 acacatctca gttggcctgg ggcgtcgctc tcggagcccg agcccacgtc ggcgagcatg
     3841 cgcgacacag catcggcgag gtgccgacgc caggcataga cgagcatcgg ctcgagttcg
     3901 tcggccaggt cgacgatcag tcgggcggtg cgctgcgcaa tcgcctcgtt gagcggcccg
     3961 tccacgtcgg ctacccccga ggacgccgtc atcgcctcgg agatgagttg cacctgccag
     4021 gccgccagcc gatcggtgtt ccgggcaaag gctcgggtca gcgcgagcgc ggtcgcctcg
     4081 tcgatgacgc tgctgcgcac cagcatgttc atccggcgca gggcccgcag gtcggcctcg
     4141 gtgaacatcc gatcgctgga ggtcgtcgag gcgaagccga gggcgtgcca gaacttccgc
     4201 gcgccgacga ccgacacccc cgccagtcgg ctgacatcgc tgcgggaaaa ctcgcggctc
     4261 gtgcccacca gcaacgacgc gacgtcgtcg ctgttcaagg agtcggcggt ccgggcggct
     4321 gcggccgtcg acggcagcat gccctcgttg tgcgccgccg gaccgaccgt catagaagca
     4381 tcatgccaac ccgccgcccc cggaagcggg ccgcacgtgc gtcacatcgc ccgcggcata
     4441 cctcgtcggt ccggaaccct cgtcgacgac gagcgcgccg tcggcatcga tgtccagtgc
     4501 gtgcgccgtc gaggtacccg ccccggggcg ctccaaccgc acctcggtgc ccagggtcga
     4561 acaggccgcg cggtatgccg cccgcacccc gtccgccgcc gccccacccg cctgcagcgc
     4621 ggcataccgg acggcgatcc ggtccagaac ggtgacgagc aggtcctcgc ggtcgaccgg
     4681 ctcggcgccc gcgagggtga gtgacgttcc cgtgtcgacg gggagttggt cccgggtctg
     4741 gctgacattg aggccgacgc cgatgacgat gagttcgtcg gccccgtcgg cgggaggacg
     4801 gtattcgcac aggattccgg cgaccttgcg gtgatcgtcc gcgggaagca gcacgtcgtt
     4861 gggccatttg agctccgctg gaaggcccgt cacggtcaag acggcttcgg ccaccgccaa
     4921 ccctgcgacc aacggcaccc accctgtcgc acccggcggg acgggcacgg agaccgagaa
     4981 ggtgagcgcg gtgaacgccg gcgcgaacca ccggcggtcc agccgccccc gcccggagaa
     5041 ctgatgctcg gcgacgacga ccgcccagac ctgcccgcgg accatcgcga gcgcgttggt
     5101 cgacatcaac tcgtcgaaga catcgaccga cgcccaggtt cgcgggggtc gacacaaacg
     5161 ggaggtgaga cgagcgacat cgaggtcact ccggttcccc atgggatgcg ccatgagtca
     5221 aggctattgt tcggatcatg tcgaacgctg gtgttcctga cgcggccgct cccaccctcg
     5281 acctgtcgac caccgccggc aagcttgccg agctgcggcg ccgggtcgcc gaagtcgcca
     5341 atgccggctc gacgactgcg gtggagaagc agcacgccaa gggcaagaag accgcccgcg
     5401 agcggatcgc gatgctcctg gacgagggca cgttcatcga gatggacaag tacgcccgcc
     5461 accggagcaa ccagttcggg caggagaagc accggcccta tggggatggc gtcgtcaccg
     5521 gctacggcca ggtcgacggt cgccagatcg cggtgttcgc ccaggacttc accgtcttcg
     5581 gcggctcgct cggcgaagtc ttcggcgaga agatcgtcaa ggtgatggac tgggccgcca
     5641 agatcggcgt ccccgtcgtc ggcctcaacg actccggcgg cgcccgcatc caggagggtg
     5701 tcgtctcgct cgggctctac ggcgagatct tcttccgcaa cgtccgctcc tcgggagtcg
     5761 tcccgcagat ctccgtcatc atgggcccgt gtgcgggcgg ggccgtctac tcccccgcga
     5821 tcaccgactt cacggtcatg gtcgacaaga cctcacacat gttcatcacc ggccccgacg
     5881 tcatcaagac cgtcaccggt gaggaggtcg agttcgagga cctcggcggc gcgcgcgccc
     5941 acaacaccaa
//
LOCUS       DAORPW010000139         5917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3667_length_5917_cov_650.353122, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000139 DAORPW010000000
VERSION     DAORPW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3667_length_5917_cov_650.353122"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLB86_10090"
     CDS             <1..317
                     /locus_tag="PLB86_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65527.1"
                     /translation="SGATMLGSGQAPFGAVRVGHGNNGQDHTEGARTHNVIGSYLHGP
                     ILPANPAVADALIAAAAERATGRPFEPEQVDDSLADEARTRQITRLRARGTGKLKDTA
                     VH"
     gene            complement(366..1109)
                     /locus_tag="PLB86_10095"
     CDS             complement(366..1109)
                     /locus_tag="PLB86_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRC65528.1"
                     /translation="MPLTLLAFHAHPDDEAILTGGTLARAAAAGHRVILVTATDGALG
                     LTSSRYAAQGLASVRAAEVRESARLLGVSRVEQLGYADSGLGPVTLDDPPGQVRFVRV
                     PVAEAAERLAAILREESVDTLLTYDVNGGYGHPDHLQVHRVGAAAAQLAQTPRVLEVA
                     VSQRVARLMKPRGLALAPVRPVTVEVDVREQLDAKVAAMRAHESQLRSDSALPRNIDL
                     LTRLPRPALERLLGRERFADLSGSSDPVL"
     gene            1148..2263
                     /locus_tag="PLB86_10100"
     CDS             1148..2263
                     /locus_tag="PLB86_10100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC65529.1"
                     /translation="MRIAMLTDCYLPRLGGIEVQVADLSARLVARGHEVEVFTLTPGA
                     VGFGQTETLDGIRVHRLGVRLPRQLLVNPLATRGLRAQLEGFDVAHIHMGVVSPFASD
                     CGFVTSRMGLPTTMTWHCVLDKAESAVRAAGIVRRWAQSGMAMNAVSDVAATPLRRIV
                     DGPPVTVLPNGIDVDAWRPTSGRPLLGDGVVRFVSAMRLEARKRPVQLVEAMAKVRAA
                     APRAGVRLEILGEGSERAKVERTVDRLGAKDWVSLPGRVPRDTLKERYAASDVFVSPS
                     VLESFGIAALEARVAGLPVVGRVGSGISEFVTDDVNGFLANDDEELVRRLTTLAVHPS
                     VRSRMTAYNLKTDPAQGWDQVVQAAEAEYARARALAG"
     gene            2260..2997
                     /locus_tag="PLB86_10105"
     CDS             2260..2997
                     /locus_tag="PLB86_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC65530.1"
                     /translation="MTAWALWLEGRLADGRVVGPWLLEHGNDPGITLWDNGLVMDELL
                     DVWSEAGEARCVLTFGVREVTDADPTPAARVPARDPDVVQAPGEVAERVQRLAAYAFV
                     HSHRGVLLAQLSDRTHAPGQWGLPGGGVDPGESPQDAVLREVAEESGQRVRLVGVLGV
                     VDGHWVGRAPDGRLEDFHAVRLVWQAVCDDPTDPVVHDVGGTTAAAAWVPIEHVASLP
                     LSDAWRGVLIDLTAPTTDGAGGSAHQT"
     gene            complement(3018..3248)
                     /locus_tag="PLB86_10110"
     CDS             complement(3018..3248)
                     /locus_tag="PLB86_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65531.1"
                     /translation="MSQPPPQIIPAPRGPNSRLLIHSLLLLLGAAALLGHLAGIEFVT
                     SVSLPWPMVLIIGGAALVVIGQLGLLRRRHRS"
     gene            complement(3245..4720)
                     /locus_tag="PLB86_10115"
     CDS             complement(3245..4720)
                     /locus_tag="PLB86_10115"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRC65532.1"
                     /translation="MTGNPADAPMPGGPAGPAGPAGPVGPSGIDVFFERLRRLGVARA
                     TQGKVAGGVCAGLARRWNIDPLVARLVFVVLTVFGGFGLLAYLVALAMLPDEKGTIFA
                     EEALRRRHGGAIFLVFIIAVMTVGEFFDRWWVWLAIPLALAAWWIVRGAAAGKSPRQL
                     GSEAKTHAAEAADAVRGWGAATPPTPAPPLSAPYQPTYQAPPPYQPYQATPVSHATPT
                     SPAADSPWGHLPQDQPAPMTAPHGMGPGRTWSPAPQAPAPLVVREKRRRGGFLVFITA
                     LGLAAITTGTLSSTNALGDRVAQPALFAVIAGCAVAALVLFVVALRGMRAPFTAFLLT
                     LVLAVSAAAAFVPNPVGFLAGAGERSWTPVSGTGSTAYALGFGQGTLDLKGISPTADT
                     TAGLSAQQVSVQLGFGELVVIVPDGVTARINLAIGAGDVVAEQPGGADPSSHLYSGGA
                     GQQTYLVGTGSPDVVVNVSVGFGSVRLQSTTAPSVIRDGVS"
     gene            4836..>5917
                     /locus_tag="PLB86_10120"
     CDS             4836..>5917
                     /locus_tag="PLB86_10120"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRC65533.1"
                     /translation="MTSSPPHARPPLARSSADAVVGGVCAGLGEFLGMPAVAVRAAAV
                     LLTVFGGGLGVAIYLVLWAVLPVSAAPAVIAESVEERRQPRFSVFHWLVLGGIVLVTG
                     GISLASDAGSWFTDARYAVPALAIAAGAFVAWYQLDDPTGRRRGRTRARWVGVVQVVV
                     GVALAMVGVVVIVTQGQGTRGVVNGALAGAAVLAGAVVIAAPFALRLYRGLQREQAER
                     VRATERADIAAHLHDSVLQTLALIQRRADDPTVVAGLARRQERELRAWLYGGESRESN
                     SLAAAVAAEIHEVEDEHGVPVEIVVTGDRTMEEAGDALVKATREAVLNAVRHGRPPVS
                     VFMEVGPSGVEVFVRDHGDGFDMSDIG"
BASE COUNT          842 a         2013 c         2208 g          854 t
ORIGIN      
        1 attccggcgc aacgatgctc ggctccggcc aggccccctt cggcgccgtc cgggttggcc
       61 acggcaacaa cggccaggac cacaccgagg gcgctcgcac ccacaacgtc atcgggtcct
      121 acctgcacgg gccgatcctc cccgccaacc ccgcggtcgc cgacgccctc atcgcggcgg
      181 ctgcggaacg ggccaccggt cggcccttcg agcctgagca ggtcgacgac tccctcgccg
      241 acgaggcccg cacccgtcag atcacccggc tgcgggcacg ggggaccggc aaactcaagg
      301 acaccgcggt tcactaggcc atcgcggccc accggttcac cgctccacga agcctcgcgg
      361 gttcgctaga gcacggggtc gctggacccg gagaggtcgg cgaaccgttc ccggcccaac
      421 aaccgttcga gggcaggccg gggcagtcgg gtcagcaggt cgatgttgcg cggcagcgcg
      481 ctgtcggagc gcagttgaga ctcgtgagcc cgcatggccg ccactttggc gtccagctgc
      541 tcccgcacgt cgacctccac cgtcaccggt cggacaggag ccaacgccag accgcgcggc
      601 ttcatcagcc tggccacccg ctgcgagaca gcaacctcca atacccttgg tgtctgggcc
      661 aattgggctg cggccgcacc gacccggtgg acctgcaggt ggtcggggtg gccatacccg
      721 ccgttgacgt cataggtgag tagcgtgtcc accgattcct cgcgcaggat ggccgcgagc
      781 cgctcggccg cctcggccac cggcacccgc acaaaccgca cctgccccgg cgggtcgtca
      841 agagtcaccg gccccaggcc gctgtcggca taccccaact gctcgacccg cgacaccccg
      901 agcagccggg cgctctcgcg cacctcggcc gcgcggacgc tcgccagccc ttgcgcggca
      961 tacctgctgc tggtgaggcc gagagcgccg tcggtggcgg tgacgaggat gacccggtgg
     1021 ccggcggcgg cggcgcgcgc gagggtcccc ccggtgagga tggcctcgtc gtcggggtgg
     1081 gcgtggaagg cgagcagtgt cagtggcatg gcgagaccca ttctccggca catgaaagag
     1141 tggggccgtg cggatcgcca tgctcaccga ctgctacctc ccgcgcctgg gcggcatcga
     1201 ggtgcaggtc gccgacctgt cggcgcggtt ggtcgcccgt ggccacgagg tcgaggtctt
     1261 cacgttgacg ccgggcgcgg tcggcttcgg tcagaccgag acactcgacg ggatccgggt
     1321 ccatcggctc ggggtccggc tgccgcgcca actcctcgtc aacccgctcg cgacccgcgg
     1381 tttgcgggct caactcgagg gtttcgacgt cgcccacatc cacatggggg tcgtcagtcc
     1441 gttcgcgtcg gactgcggct tcgtcaccag tcggatgggc ctgccgacga cgatgacctg
     1501 gcactgcgtc ctggacaagg cggagtcggc cgtgcgcgct gccgggatcg tccggcgttg
     1561 ggcgcagtcg ggaatggcga tgaacgccgt ctccgacgtc gccgccacac cgctgcgccg
     1621 gattgtcgat gggccaccag tcacggtgct gcccaacggt attgacgtcg acgcgtggcg
     1681 ccccaccagc ggccgcccac tgctcggcga cggcgtcgtg cgcttcgtct cggcgatgcg
     1741 cctggaggcc cgcaagcgcc ccgtccaact cgtcgaagcg atggccaagg tgcgcgccgc
     1801 agcgccgcga gcaggcgtgc gcctggagat cctcggtgag gggtccgagc gcgcgaaggt
     1861 cgagcgcacg gtcgaccggt tgggcgcgaa ggattgggtg tccctgcccg gccgggtgcc
     1921 gcgcgacacc ctcaaggaga ggtatgccgc gagcgacgtt ttcgtctcgc cgtcggtcct
     1981 cgagtccttc ggtatcgcgg cgctggaggc tcgggtcgct ggcctgccgg tcgtcggacg
     2041 ggtggggtcg ggcatctcgg agttcgtcac ggacgatgtc aacggcttcc tggccaacga
     2101 cgacgaggag ttggtgcgtc ggttgacgac gctggccgtc cacccgagcg tgcggtcccg
     2161 gatgacggca tacaacctca agaccgaccc ggcgcagggc tgggatcagg tcgtgcaggc
     2221 ggccgaggcc gaatacgccc gggcgcgcgc gctcgccgga tgaccgcctg ggccctgtgg
     2281 ctggaagggc gcctggccga cggtcgggtc gtggggccct ggctgcttga gcatggcaac
     2341 gaccctggaa tcacgctgtg ggacaacggt ttggtgatgg atgagttgct cgacgtttgg
     2401 tctgaggcag gcgaggcccg ctgcgttctg accttcggcg ttcgagaagt caccgacgcg
     2461 gacccgacac ctgcagcgcg cgtgccggcg cgtgacccgg acgtggtgca ggctccgggg
     2521 gaggtggccg agcgcgtgca gcggctcgcg gcatacgcct tcgtgcactc gcaccgcgga
     2581 gtgctgctgg cccaactgtc cgaccgcacc cacgcgcccg ggcagtgggg gttgcccggt
     2641 ggtggtgtcg acccggggga gtcgccgcaa gatgcggtgt tgcgtgaggt cgccgaggag
     2701 agcggtcagc gggtgcgcct ggtcggtgtg ctcggtgtcg tggacggtca ctgggtcggg
     2761 cgcgcacccg acggccggtt ggaggacttc cacgccgtgc ggctggtgtg gcaggcggtg
     2821 tgtgacgacc cgaccgaccc ggtggtccac gacgtcggcg ggacgacggc tgcggccgca
     2881 tgggtgccca tcgagcacgt ggcgagcctg ccgctcagtg acgcatggcg tggcgtgctc
     2941 atcgatctga ccgctccgac cacggacggc gccgggggct cagcccacca gacatgagcc
     3001 tggggggccg ccgcccgtca ggaccggtgg cgtcggcgca gtagccccaa ctgcccgatg
     3061 acgaccaggg cagcgccgcc gatgatgagg accatcggcc acggcagcga caccgaggtg
     3121 acgaactcga tcccggcgag gtggcccagc agggccgcgg cgccgaggag tagcagcagt
     3181 gagtggatca gcaggcgcga gttgggaccg cgcggggcgg ggatgatctg gggcggtggc
     3241 tgactcatga gactccatcc cggatgactg acggggccgt ggtgctctgc aaccggacgc
     3301 tgccgaagcc gacgctgacg ttgacgacga cgtctggtga tccggtgccg acgagatagg
     3361 tctgctggcc cgccccgccg gagtagaggt ggctcgacgg gtcagcaccg ccgggctgct
     3421 cggcgacgac gtcgcccgct ccgatggcta ggttgatgcg ggcggtgacc ccgtcgggca
     3481 cgatgacgac gagctcaccg aaccccaact gcacggagac ctgctgggcg ctcaagcccg
     3541 ccgtggtgtc ggccgtgggg gagatcccct tcagatcgag ggtcccctga ccgaaaccca
     3601 gcgcgtatgc cgtggagccg gttcccgaga cgggcgtcca ggagcgctcc ccggcaccgg
     3661 ccaggaagcc gacggggttg gggacgaagg cggctgcggc gctgacggcc aggacgagcg
     3721 tcaggaggaa cgccgtgaag ggggcccgca tgccgcgcag agcgacgacg aagagcacca
     3781 gtgcggcgac ggcgcagccg gcgatgaccg cgaagagggc gggttgcgca acccggtcgc
     3841 ccagcgcatt ggtcgagctc agcgttcccg tggtgatggc ggccagcccc agggcggtga
     3901 tgaagacgag gaatccaccg cggcggcgct tctcgcgcac gactagcggg gcaggcgcct
     3961 ggggagcggg gctccacgtc ctgccgggcc ccattccgtg tggcgcggtc atcggcgccg
     4021 gctggtcttg cggcagatgc ccccagggcg agtcggcggc gggcgacgtg ggagtcgcgt
     4081 gagacaccgg ggtggcctga tacggctgat acggcggggg tgcctgatac gtcggctgat
     4141 acggcgcaga caggggaggc gctggagtcg gcggtgtcgc ggcgccccac ccgcggaccg
     4201 cgtcggctgc ctcagcagcg tgcgtcttcg cctcagaccc cagttggcgc ggcgacttgc
     4261 ccgcagccgc gccgcgaacg atccaccagg cggccagcgc caaggggatc gccagccaca
     4321 cccaccatcg gtcgaagaac tctccgaccg tcatgacggc aatgatgaag accaggaaga
     4381 tggcaccccc gtggcggcga cgcagcgcct cctcggcaaa gatcgttccc ttctcgtccg
     4441 gcagcatggc caacgcgacc aggtaggcca gcaggccgaa gccgccgaag accgtgagga
     4501 cgacgaagac caggcgcgcg acgagcgggt cgatgttcca ccgtcgagcc aggcccgcac
     4561 agactccgcc ggcgaccttg ccctgcgttg cccgagccac tccgagccgg cgcagccgct
     4621 cgaagaagac gtcgatcccg ctgggaccga ccggacccgc gggaccagcc gggccggcag
     4681 ggccgccggg catcggcgcg tcagcagggt ttcccgtcat gcccctatcg tcgctggtcg
     4741 gaggcccgcc ctgccatgag ggtggaccct gaaagcaccc tgaacccgcc ccggtgaggg
     4801 gagcagacgg catacccatg ccccaagatg gggacgtgac ttcctcgccg ccgcatgctc
     4861 gccccccgtt ggcgcgctcc agcgcggacg cggtcgtcgg cggcgtgtgc gcggggctgg
     4921 gtgagttcct gggtatgccg gccgtcgccg tccgcgcggc ggccgtcctg ctcacggtgt
     4981 tcggtggtgg cttgggcgtc gcgatctacc tggtgctgtg ggcggtgctg cccgtgtcgg
     5041 ctgcccccgc ggtcatcgcc gagtcggtcg aggagcggcg gcaaccgcgg ttctcggtct
     5101 tccattggct cgttctcggc ggaatcgtcc tggtcaccgg gggcatctcg ctcgccagtg
     5161 atgcggggtc gtggttcacc gacgcgaggt atgccgtccc cgccctggcc atcgcggccg
     5221 gcgcgttcgt cgcgtggtac caactggacg accccaccgg gcgtcgccgc ggccggaccc
     5281 gagcccgttg ggtcggcgtc gtgcaggtcg tcgtcggcgt cgcgttggcg atggtcggcg
     5341 tcgtcgtcat cgtgacgcag gggcaaggca cgcgtggggt tgtcaacggc gcgctcgccg
     5401 gtgccgccgt gctcgcgggg gcggtcgtca tcgcggcgcc gttcgcgctg cggctctatc
     5461 gcgggctgca gcgcgagcag gccgagcggg tgcgggcgac cgagcgggcc gacatcgctg
     5521 cgcacctgca tgattcggtc ctgcagaccc ttgcgctcat ccagcgccga gccgatgacc
     5581 cgacggtggt cgccgggctg gcccgacgcc aggagcgcga gttgcgggcc tggctctatg
     5641 gcggagagtc ccgcgagagc aacagcctgg ctgccgctgt tgctgccgag atccacgagg
     5701 tcgaggacga gcacggggtg ccggtcgaga tcgtcgtgac cggcgatcgg acgatggagg
     5761 aggccggcga cgcgctggtc aaggccaccc gcgaggcggt gctcaacgcg gtgcggcacg
     5821 gtcgtccgcc ggtgtcggtg ttcatggagg tcgggccgag tggcgtcgag gtgttcgtcc
     5881 gcgaccacgg cgacggcttc gacatgagcg acatcgg
//
LOCUS       DAORPW010000140         5854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3744_length_5854_cov_674.992412, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000140 DAORPW010000000
VERSION     DAORPW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3744_length_5854_cov_674.992412"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PLB86_10125"
     CDS             <1..717
                     /locus_tag="PLB86_10125"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HRC65534.1"
                     /translation="LREQVPYLAEVCTRTVNDRKGGHLARRRSDGRIVLRDNAMVVPG
                     EEHFFADETRHSTFHANNLWVDLDVVARMLDERAGVLGLPIIVNRKTVDPTDKSSTKV
                     IQIECAMGTAIEAIDGSRAMHVPRSRFRPVKTTNELTLVRSDLFRFEPDWRVAATTER
                     PDPLIDLSSEFAFVDDLERLFPAGVPSLRECDSLRVRGDVTFGAGVVCRGDVLVQGPV
                     AVPDAAVLTAESAGASGSGG"
     gene            710..1930
                     /locus_tag="PLB86_10130"
     CDS             710..1930
                     /locus_tag="PLB86_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRC65535.1"
                     /translation="MADRTVEQHLAAVLAVARPLPPAALPLGAAVGLVAAANVVCLVD
                     LPGFDNSAMDGYAVRAADLLGATPNTPVRLPVVADLAAGDAPARPVGPGEAARIMTGA
                     MVPDGADTIVKVEDTDAGMPVVAVFVETPLGTSIRRRGEDLPVGGPVLASGTVLTARH
                     VGLLAASGHATVTVHPRPRVAVVSTGAELIAPGKPLQPGQIHDSNSYLLAALVEAAGG
                     HTAYRGSIGDHPDEVRALLDRLATEVDVIVTTGGVSMGVHDVVKAVLRERGEVDFVTV
                     AMQPGKPQGFGVLGERRVPFFGLPGNPVSAFVSFEAFVRPLLRRLLGLEPALAPTTRV
                     RLTAALRSPAGRMQFARATVSRDPHDGRWLATPVAGQGSHFVADLAAADALLLVPAEV
                     THLQVGDEVESILL"
     gene            1927..2427
                     /gene="moaC"
                     /locus_tag="PLB86_10135"
     CDS             1927..2427
                     /gene="moaC"
                     /locus_tag="PLB86_10135"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRC65536.1"
                     /translation="MTDPVSEAAPGLTHVRADGSAHMVDVSAKPITAREASAAGRVEL
                     SPAAVAALRGGTVPKGDALAVARIAGLQAVKRTPELIPLAHPVAVHAASVDLSVDDAG
                     VEIRATVRTADRTGIEMEALTAVAVAALALVDMVKAVDRHARITEIRVTAKSGGRSGD
                     WREPGS"
     gene            2424..2924
                     /locus_tag="PLB86_10140"
     CDS             2424..2924
                     /locus_tag="PLB86_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRC65537.1"
                     /translation="MSSAPPRRAVVIVASTRAADGTYEDRTGPTLVDALTTWGYAATG
                     PIVVSDGDPVGLALATALRDGPEVVLTTGGTGVSPSDRTPEQTRPFLDRELPGIPELI
                     RAQGFAGGQPLAAISRGLAGVSGRTLVVNLPGSRGGVRDAIDVLGPILDHVVEQLAGG
                     DHEAVG"
     gene            2942..3532
                     /locus_tag="PLB86_10145"
     CDS             2942..3532
                     /locus_tag="PLB86_10145"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRC65538.1"
                     /translation="MSLRVADGLTPSLVLRPLHRRDQSAWEALRRDNTAYLSRWEPTP
                     PDGTIVRTGFGGYVRSLNRDARAGNIAPFAVEVDGELAGQVHLFGVMRGSLLSGAAGY
                     WLGERFAGQGVATRALAALIDYALGPFGLHRVEVNIRPENVASLAVVAHLKFREEGVR
                     ERYLHIEGDWRDHRTFALTVDDLGGLPTIERWHLPG"
     gene            3624..4697
                     /locus_tag="PLB86_10150"
     CDS             3624..4697
                     /locus_tag="PLB86_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65539.1"
                     /translation="MQPSSLIFVAIIAIWAAFLVQHWVRRREALSTARSVDRFSEAIR
                     LLDRTPARVSAEPRPAPQVYAALASPLVARSGGGSGAVVAAGSGAAAGVGSGSGAAAA
                     EVLAGPAPAGPRPTATAPNRRGSRLALWGRRVRGLSFLGLVALVPVTVVLSALSVLRW
                     TSVLVAVLSLVLGLVMLRYAAVHERTLAKVGRSMAASELSGQRAAAATGSAARPAPRR
                     RSAAPVRRVVVTPGGAAPAVAPAASAADAPAQETSESATATADQTPAAQTPAALTPAV
                     QVPAAAAVTIEPAGERASRRAAYAQYSADGTWQPVAVPPPTYTLKARAAHPQPEPAEV
                     TATPEPMVAESAADDTHVRAVGD"
     gene            complement(4716..>5854)
                     /locus_tag="PLB86_10155"
     CDS             complement(4716..>5854)
                     /locus_tag="PLB86_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRC65540.1"
                     /translation="RTAYPVAMSSTPSPIPAPVRAASEWAWRLLAIAGAVVAFAYLLG
                     FVSEAVIPVIVAVLLAAMLNPVNQWLRARLGRPAPAAALTVLATILAIGALLFLVGSQ
                     LTGGFPQMAAQVGTGIETIKTWLQTSFKISDTQFTQYLDDAKNALQTSEGLRSGLTKA
                     GLGASHVVAGLFISLFALFFFLFEGARIWGWVVRLFPRTARVRVAGAGEVAWTQLTAF
                     TRATILVALVDAVGITVVALLLRVPFAPAIGAIVFLGAFIPIVGALASGMVAVLLALV
                     AHGPIAALLMLAGVLAVQQIESHVLQPILLGRAVSVHPLAVILAITAGVVVAGVVGAL
                     VAVPLAAVVNAVVKQLTGSGAASGESMGSESGGEPDEDPALSPS"
BASE COUNT          774 a         2139 c         2080 g          861 t
ORIGIN      
        1 ttgcgcgaac aggtgcccta cctcgccgag gtctgcaccc gcacggtcaa cgaccgcaag
       61 ggcggacacc tcgcccgccg ccgcagcgat ggacgaatcg tgttgcggga caacgcgatg
      121 gtcgtccccg gtgaagagca cttcttcgcc gacgagaccc gacacagcac cttccacgct
      181 aacaacctgt gggtcgacct cgacgtcgtc gccaggatgc ttgacgagcg cgccggggtg
      241 ctggggctgc cgatcatcgt caaccgcaag accgtcgacc cgaccgacaa gtcctcgacg
      301 aaggtcatcc agatcgagtg cgccatgggg acggccatcg aggcaatcga cggttcgcgg
      361 gcgatgcatg tgccgcgcag ccggttccgg ccggtgaaga cgaccaacga gctcaccctc
      421 gtgcgctcag acctgttccg gttcgagccc gactggcggg tggcggcgac gaccgaacgt
      481 cccgatccgc tcatcgatct gtcgagcgag ttcgcgttcg tcgacgacct tgagcggctc
      541 ttcccggccg gggtgccctc cctgcgtgag tgcgactcct tgcgggtgcg tggcgacgtc
      601 accttcgggg ccggggtggt ctgccggggt gacgtgctcg tgcagggccc cgtcgccgtg
      661 cccgacgccg ccgtgctgac ggctgagtcg gccggcgcga gcgggtccgg tggctgaccg
      721 cacggtcgag caacacctgg cagcggtcct cgcggtcgct cgcccgctgc cgcccgccgc
      781 cctcccgctc ggcgcggcgg tcggtctcgt ggctgctgcc aatgtcgtct gcctcgtcga
      841 ccttcccggc ttcgacaact ccgcgatgga tgggtatgcc gtgcgcgcgg cggacctgct
      901 cggagcgacc ccaaacaccc ccgtgcggtt gcccgtcgtg gccgatctgg ccgccggcga
      961 cgcccccgca cgcccggtcg gccccggcga ggcggcccgg atcatgaccg gagccatggt
     1021 ccccgatggc gccgacacca tcgtcaaggt ggaggacacc gacgccggta tgccggtcgt
     1081 cgccgttttc gtcgagaccc cgctcggcac gtcgatccgt cggcgcggtg aggacctgcc
     1141 cgtcggcggc ccggtgctgg cgtccgggac cgttctcacg gcccgccatg tcggtctgct
     1201 cgcggcctct gggcacgcca ccgtcacggt ccacccccgc cccagggtgg ccgtcgtctc
     1261 gaccggtgcc gagctcattg ccccgggaaa accgttgcag cctgggcaga tccacgattc
     1321 caacagctac ctgctcgctg ccctcgtcga agcggcaggt gggcacacgg cataccgggg
     1381 gagcatcggc gaccaccccg acgaggtgcg tgcgctgctg gaccggctgg ccaccgaggt
     1441 cgacgtcatc gtcaccaccg gcggggtcag catgggggtc cacgacgtcg tcaaggcagt
     1501 gttgcgcgag cgcggcgagg tcgacttcgt caccgtggcc atgcaaccgg gcaagccgca
     1561 ggggttcggg gtgctgggcg agcggcgggt gccgttcttc gggctgccgg gcaatccggt
     1621 gtcggcgttc gtgtcgttcg aggcgttcgt ccggccgctg ctgcgccggc tgctcgggct
     1681 cgagccggcg ctcgcgccga ccacccgggt gcgcctcacc gcggcgctgc gctcgcccgc
     1741 gggccggatg cagttcgccc gcgccaccgt gtcccgcgac ccgcacgacg ggcggtggct
     1801 ggcgaccccc gtggccgggc agggctcgca cttcgtcgcc gacctggccg cggccgacgc
     1861 gctgctgctc gtgcccgccg aggtcaccca cttgcaggtg ggcgacgagg tcgagagcat
     1921 cctcctatga ccgacccggt gagcgaggcg gcgccggggc tgacccatgt ccgcgccgat
     1981 ggcagcgcgc acatggtcga cgtcagcgcg aagccgatca cggcccgcga ggcctcggcc
     2041 gccggacggg tggagttgtc ccccgcagcg gtggcggccc tgcgcggggg caccgtgccg
     2101 aagggcgatg ccctcgccgt cgcgcggatc gccgggctgc aggccgtcaa acgcaccccc
     2161 gagctcatcc cgctcgcgca tcccgtcgcg gttcacgccg cctccgtcga tctgtcggtc
     2221 gacgatgcgg gcgtggagat ccgggccacg gtccgcacgg ccgaccggac cgggatcgag
     2281 atggaggccc tcacggctgt tgcggtcgct gccctggccc tcgtcgacat ggtcaaggcc
     2341 gtcgaccggc acgcgcgaat caccgagatc cgggtcaccg ccaagtctgg tgggcgctcc
     2401 ggtgactggc gggagccggg ctcgtgagct cagcgccccc gcgccgagcg gtggtcatcg
     2461 tcgcctccac ccgggccgcc gacggcacct acgaggaccg caccggcccc accctcgtcg
     2521 acgccctcac cacctggggg tatgccgcca ccggcccgat cgtcgtctcc gacggtgacc
     2581 cggtcggctt ggccttggcg accgcccttc gcgacggacc ggaggtcgtc ctcacgaccg
     2641 gcggcaccgg ggtgtccccg agcgaccgga cccctgagca gacccgaccg ttcctcgatc
     2701 gcgagctgcc cggcataccg gagctgatcc gggcccaggg gttcgccggc ggacagccgc
     2761 tggccgcaat ctcgcggggg cttgcgggcg tgagcggccg gaccctcgtg gtcaacctgc
     2821 ccggctcgcg cggcggggtg cgcgatgcga tcgacgtcct cgggccgatc ctggatcacg
     2881 ttgtcgagca actcgccggc ggcgaccacg aggctgtcgg atgagccccg ggcgatggcc
     2941 cgtgagcctc cgggtcgccg acgggctgac cccgtcgttg gtcctgcggc cgttgcaccg
     3001 ccgcgaccag tcggcgtggg aggccctgcg ccgggacaac acggcatacc tctcccgctg
     3061 ggagcccaca ccccctgacg ggacgatcgt gcgaaccggc ttcggtgggt atgtccgctc
     3121 cctcaaccgg gacgcgcggg ccggaaacat cgcgccgttc gccgtggagg tcgacgggga
     3181 gttggccggg caggtccacc tcttcggggt gatgcgcggg tcgctgctct ccggggcggc
     3241 cggctattgg ctgggggagc gtttcgctgg gcaaggcgtg gccacccggg cgctcgcggc
     3301 gctgatcgac tatgcccttg gtccgttcgg gctgcatcgg gtcgaggtca acatccggcc
     3361 cgagaacgtc gcgtcgttgg ccgtcgtcgc gcacctgaag ttccgcgagg agggggtgcg
     3421 agagcgttac ttgcacatcg agggggactg gcgcgatcac cggaccttcg cgctgacggt
     3481 cgatgacctc ggtggcctcc cgacgatcga acggtggcat ttaccgggct gatcggcgtt
     3541 ttcacagcag tcacacgcgc ctcatccgcg acacaccggg ccacgtccgc cggggtgccg
     3601 tcaggggtcc atacggtagt cgcgtgcagc cctcgtccct gatcttcgtc gccatcattg
     3661 cgatctgggc tgccttcctc gtccagcact gggtgcgccg ccgcgaggcc ctctcgaccg
     3721 cgcggtccgt cgaccggttc agcgaggcca tccgcctgct cgaccgcacc cccgcgcggg
     3781 tgtccgccga gccccgccca gccccccagg tgtatgccgc cctcgcctcg ccgctcgtgg
     3841 cccggagtgg tggcggttct ggtgctgttg ttgctgctgg gtctggtgct gcggctggtg
     3901 ttgggtctgg gtctggtgct gcggcagccg aggtgctcgc cgggcccgcg cccgctggtc
     3961 ctcgccccac ggcgacggcc ccgaaccgcc gaggttcccg acttgcgctg tggggtcgcc
     4021 gcgtccgcgg gctgtccttc ctcgggctgg tggcgctcgt tccggtcacc gtcgtgctct
     4081 cggcgctatc ggtgttgcgc tggacctcgg tcctcgtcgc ggtcctctcg ctggtcctcg
     4141 gtctggtcat gctccggtat gccgccgtcc acgagcgcac cctggccaag gtcggacggt
     4201 cgatggccgc ctccgaactc tcggggcagc gtgccgccgc agcgaccggg tcggctgctc
     4261 ggccggcgcc ccggcgacgg tcggccgctc cggttcgccg cgttgtcgtg acgccgggcg
     4321 gtgccgcacc cgccgttgcc cccgcggcct cggctgctga cgctcccgcc caggaaacct
     4381 ccgagagcgc gacggcgacg gctgatcaga cgcctgccgc tcagacccct gctgcgctga
     4441 cccctgcagt tcaggtgccg gctgcggccg cggtcacgat cgaacccgcc ggagagcggg
     4501 cctcgcggcg cgcggcatac gcccagtatt ccgccgacgg gacctggcag ccggtggctg
     4561 tcccgccgcc tacttacacg ctcaaggcac gcgctgccca cccgcagccc gagcctgccg
     4621 aggtgaccgc gacccccgag ccgatggttg ccgagtcggc cgcggacgac acccacgtgc
     4681 gagccgtcgg cgactgaccc ggccgagtgc gccgatcagg agggcgacag cgcggggtcc
     4741 tcgtcgggct ccccgcccga ctcgctgccc atgctctcgc ctgaggcggc accggagccg
     4801 gtgagttgtt tgacgaccgc gttgaccacg gcggccaggg gcactgcgac gagcgcgccg
     4861 acgacccctg caacgacgac gccagcggtg atcgcgagga tcaccgcaag ggggtgcacg
     4921 ctcaccgctc ggccgagcag gatcggctgg agcacgtgcg actcgatctg ctggaccgcg
     4981 agaaccccgg caagcatgag caaagcggcg atcggaccgt gcgcgacgag cgcgagcagc
     5041 acggccacca tgccggacgc gagcgcgccc acgataggga tgaacgcgcc gaggaagacg
     5101 atcgcaccga tcgccggggc gaacggcacc cgcaggagca gagcgaccac ggtgatgcca
     5161 acagcgtcga cgagggccac gaggatggtc gcgcgcgtga aggcggtgag ctgagtccag
     5221 gcgacctctc ctgcgccggc gacccgcacc cgggccgtcc gcgggaacaa ccggacgacc
     5281 cacccccaga tccgggcacc ctcaaagagg aagaagaaca gcgcgaacag gctgatgaac
     5341 agcccggcaa cgacgtgcga ggccccgagt ccggccttgg tgagccccga gcgcagcccc
     5401 tcacttgtct gcaacgcgtt cttggcgtcg tcgagatact gggtgaactg cgtgtcgctg
     5461 atcttgaagc ttgtctgcag ccaggtcttg atcgtctcga tcccggtgcc gacctgcgcg
     5521 gccatctgcg ggaagccgcc ggtcaactgg ctgcccacga ggaacagcag tgccccgatc
     5581 gcgaggatcg tcgccaggac cgtgagcgcg gcggccgggg cgggccgccc gagtcgggcc
     5641 cgcagccact ggttgaccgg gttcagcatc gcggcgagca gcaccgcgac gatgaccggg
     5701 atcacggcct cgctgacgaa gcccagcagg taggcgaacg cgacgaccgc gcccgcgatc
     5761 gccagcaggc gccaggccca ctcgctcgcg gcgcggaccg gggcggggat cggtgagggg
     5821 gtgctcgaca tggccacagg gtatgccgtg cgcc
//
LOCUS       DAORPW010000141         5826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3777_length_5826_cov_647.278115, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000141 DAORPW010000000
VERSION     DAORPW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3777_length_5826_cov_647.278115"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2597
                     /gene="metH"
                     /locus_tag="PLB86_10160"
     CDS             <1..2597
                     /gene="metH"
                     /locus_tag="PLB86_10160"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRC65541.1"
                     /translation="IDVNMDEGMIDGVAAMDRFLKLVASEPDICRVPVMVDSSKWEVI
                     EAGLKTIQGKPIVNSISMKEGVEAFVKHARLCRKYGAAIVVMAFDEQGQADNLARRRE
                     ISAAAYRILVDEVGYPAEDIVIDPNVFAVATGIEEHSAYGIDFIEATRWIKENLPGAL
                     VSGGVSNVSFSFRGNNPVREAIHAVFLLHAIRAGMDLGIVNAGALVVYDEIDAELRER
                     IEDVILNRRADAAERLLEVAERFNVAGTQAEVADEAWRSLPVGQRITHALVKGLDAHI
                     EADTEELRADLAASGGRPIEVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKKAV
                     GYLVPFIEAERLAAGGSADDAEAHTNGTIVMATVKGDVHDIGKNIVGVVLQCNNYEVI
                     DLGVMVPAQKIIDAAREHRADIIGLSGLITPSLDEMVTFAAELQRLGLTLPLLIGGAT
                     TSKAHTAVKVSPRYDGPVIWVKDASRSVPVAAALLSDEQRPGLLVDVKADYDAIRVRH
                     AAKAGDRQLISLEAARANATPVDWAAYKPLRPHLLLQQARDVSAATSGANPTHVTQFT
                     RVFRDYPVAELREYIDWQPFFAAWELKGRFPDLLNNPASGETARKLYADALTMLDRIE
                     AERWLRPAGVIGLFPANSVGEDIEVYAAESRSEVRTVLHHLRQQGAHRDGIPNRSNAD
                     FVAPKSTGLRDYMGAFAVTAGVDLPARVAAFKADLDDYSAILLESLADRLAEAFAERL
                     HQRVRREFWGYHPGERLGNEALIAEDYQGIRPAPGYPACPDHTEKALLWDLLDVEAAT
                     GMVLTESMAMWPGASVSGWYFAAPHAQYFVVGRLGRDQIAEYADRKGWTIAQAERWLS
                     PNLGYDPE"
     gene            complement(2628..3719)
                     /locus_tag="PLB86_10165"
     CDS             complement(2628..3719)
                     /locus_tag="PLB86_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC65542.1"
                     /translation="MATAIRVEMLPARLGDCLLVECLRPGRRPWRMLVDGGPPDTWPL
                     LKARLDRLPASNPAIDVAVVTHIDSDHIGGMIPFLASDFARTHVGDVWFNGHRQASSP
                     RAVPRSVAQGETLTATLSGAPGAQPGMPWNVAFAGAAIATPVDPGFAEVTIPGGPRIT
                     VLSPNGRRLAALAKVWTEALDKALHPEPDRPVPDLPGPLGDLATLAARKTSKDGTPPN
                     GSSIALLVEHRGASVLLGADAFGPVLTDALTALAGARGIPAVAVDAFKLPHHGSEANV
                     LADLVAAAPATHYLVSSNGDTFGHPDDPAIARVVTGAPPGATVWFNYRNARTERWADP
                     ALRTAHGYAVRYPSDPAAGVLVKLPVRAG"
     gene            3823..4668
                     /locus_tag="PLB86_10170"
     CDS             3823..4668
                     /locus_tag="PLB86_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRC65543.1"
                     /translation="MVAALSPSRASDFKQCPLLYRFRVIDRLPSPTSAAAARGTLVHA
                     VLERLFDLPAHERTPAAAAALVAPQWERLVAEEPELAGLLDADDDGAADAWYAQAVGL
                     VERWFTLEDPTRLEPAERELYVEAEVDGLVLRGYVDRLDVAPDGSMRVVDYKTGRSPS
                     ELFERSSLFQMKFYALVLWRLRGVVPRMLQLVYLGNGEVVRYEPDEADLLATERGIKA
                     LWAAIERAAATGDWRPRVSRLCDWCDHKSLCPEWGGTPPPLPTDAPARALDPAVTGRV
                     VPADD"
     gene            complement(4703..5383)
                     /locus_tag="PLB86_10175"
     CDS             complement(4703..5383)
                     /locus_tag="PLB86_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRC65544.1"
                     /translation="MSARLPAAVLWDLDGTLVDSEAYWVLEERALVEDFGGVWTEADA
                     FALVGRALTDSAAYIKDHSPVDLPIDELVDRLVTGVVRRIRAEVPWRPGARELLTALR
                     EAGVPTALVTMSYRPITDAIGDALPGGAFDLIVSGEDVTHGKPHPEPYLRGAAGLGLA
                     PEECIAIEDSEPGARSAAAAGAQTIVVPSVKPVPRMPGVVQIPTLVGVSPTDLVGLPG
                     FAALPTRS"
     gene            5563..>5826
                     /locus_tag="PLB86_10180"
     CDS             5563..>5826
                     /locus_tag="PLB86_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HRC65545.1"
                     /translation="MLRSIPELREPVLVAAFEGWNDAGEAASAAAAHLIEVWDAQQVG
                     ELDPEDYYDFQVNRPKITLVDGQRRITWPTTRIFVCQGTTGPHD"
BASE COUNT          860 a         1984 c         2096 g          886 t
ORIGIN      
        1 tcatcgacgt caacatggac gaggggatga tcgacggggt cgcggcgatg gaccgcttcc
       61 tcaagctcgt ggcaagcgaa ccggacatct gccgggtgcc ggtcatggtc gactcgtcca
      121 agtgggaggt catcgaggcc ggcctcaaga ccatccaggg caagccgatc gtcaactcga
      181 tctccatgaa ggaaggcgtc gaggcgttcg tcaagcacgc ccggctctgc cgcaaatacg
      241 gcgccgccat cgtcgtcatg gccttcgacg agcagggcca ggccgacaac ctggcgcgcc
      301 gacgcgagat cagcgccgcg gcataccgga tccttgtcga cgaggtcggc tacccggccg
      361 aagacatcgt catcgacccc aacgtcttcg cggtggcgac cggcatcgag gagcactcgg
      421 cctacgggat cgacttcatc gaggccaccc ggtggatcaa ggagaacctc cccggcgcgt
      481 tggtgtccgg cggcgtctcc aacgtgtcct tcagcttccg cggcaacaac ccggtccgtg
      541 aggcgatcca cgcggtgttc ctcctgcacg cgatccgcgc cgggatggac ctcgggatcg
      601 tcaacgccgg tgccctggtc gtctacgacg agatcgacgc cgagctgcgc gagcgcatcg
      661 aggacgtcat cctcaaccgc cgggccgacg ccgccgagcg cctgctcgag gtggccgagc
      721 gtttcaacgt cgccggcacc caggccgagg tcgccgacga ggcctggcgg tccctgcccg
      781 tggggcagcg gatcacgcac gccctcgtca agggtctcga cgcgcacatc gaggccgaca
      841 ccgaggagtt gcgtgccgac ctcgccgcca gcggtggccg accgatcgag gtcatcgaag
      901 gcccgctgat ggacggcatg aacgtcgtcg gcgacctctt cggcgccggc aagatgttcc
      961 tcccccaggt cgtcaagtcg gctcgggtga tgaagaaagc cgtcggctat ctcgtcccct
     1021 tcatcgaggc cgagcggctc gcggcgggcg gcagcgccga cgacgcggaa gcgcacacca
     1081 acggcacgat cgtcatggcg acggtcaagg gcgacgtgca cgacatcggc aagaacatcg
     1141 tcggggtcgt cttgcagtgc aacaactacg aggtcatcga cctcggcgtc atggtgcccg
     1201 ctcagaagat catcgatgcc gcccgcgagc accgggccga catcatcggg ctctccggcc
     1261 tcatcacgcc cagcctcgac gagatggtca ccttcgccgc cgagttgcag cggctcgggc
     1321 tgacgctgcc gttgctcatc ggcggggcga cgacctccaa ggcccacacg gccgtcaagg
     1381 tgtccccgcg ctacgacggg ccggtcatct gggtcaagga cgcctcccga tcggtgccgg
     1441 tggcagccgc gctgctctcc gacgaacagc gacccgggct gctggtcgac gtcaaggccg
     1501 actacgacgc gatccgggtc cggcatgccg ccaaagccgg tgaccggcag ttgatctcgt
     1561 tggaagcggc ccgggccaac gcgaccccgg tggactgggc ggcatacaag ccgctgcggc
     1621 cccacctgct gctgcagcaa gctcgagacg tctccgcggc aacctcgggc gcgaacccca
     1681 ctcacgtcac ccagttcacc cgcgtgttcc gcgactaccc cgtggccgag ttgcgcgaat
     1741 acatcgactg gcagccgttt ttcgccgcgt gggagctcaa gggtcgcttc cccgacctgc
     1801 tcaacaaccc cgccagcggc gagaccgcgc gcaagctcta cgccgacgcg ctgaccatgc
     1861 tcgaccggat cgaggctgag cgttggctgc ggccggcggg cgtcatcggg ctgttccccg
     1921 ccaactccgt gggcgaggac atcgaggtgt atgccgcgga gtcccgttcc gaggtccgca
     1981 ccgtcctgca ccacctgcgt cagcaggggg cgcaccgcga cggcataccg aatcggtcca
     2041 acgccgactt cgtcgccccg aagtccactg gcctgcgcga ctacatgggc gccttcgcgg
     2101 tgaccgctgg cgtcgacctg cccgcccggg tggccgcgtt caaagccgac ctggacgact
     2161 attcggcgat cctgctggag tccctcgccg accggctcgc cgaggccttc gccgagcgtc
     2221 tgcaccagcg ggtccggcgt gagttctggg gttaccaccc cggggagcgg ctgggcaacg
     2281 aggccctcat cgccgaggac taccaaggca tccggccggc tccgggctac ccggcctgcc
     2341 ccgaccacac cgagaaggcc ctcctgtggg acctgctcga tgtcgaggcg gccacgggga
     2401 tggtgctcac cgagtcgatg gcaatgtggc cgggagcctc ggtgtccggg tggtacttcg
     2461 ccgcaccgca cgcgcagtac ttcgtggtcg gccgccttgg gcgcgaccag atcgccgagt
     2521 atgccgaccg caaggggtgg acgatcgccc aggccgagcg ttggctgtcg ccgaacctcg
     2581 gctacgaccc ggagtaggcc ggggtaagtc ggagtgggcc ggagtagtca gcccgctcgc
     2641 accggaagct tcaccaggac cccggcggcg gggtcgctgg gatagcgcac ggcataccca
     2701 tgggcggtcc tcagcgcggg gtcggcccaa cgctcggtgc gggcgttccg gtagttgaac
     2761 cacacggtcg cccccggcgg tgctccggtg acgacccgcg cgatcgccgg gtcgtctggg
     2821 tggccgaagg tgtcgccgtt gctcgagacg aggtagtgcg tcgcgggcgc ggccgcgacg
     2881 aggtcggcga ggacgttcgc ctcactgccg tggtgcggca gtttgaaggc atcaaccgca
     2941 accgcaggta tgccgcgcgc cccggcgagg gcggtcagcg catcggtgag gacgggtccg
     3001 aacgcgtcgg cgccaaggag cacgctggcg ccgcggtgct cgacgagcag ggcgatgctg
     3061 ctgccgttgg gtggggtgcc gtccttcgag gtcttgcggg cagcgagggt ggccagatca
     3121 cccaacgggc cgggcaggtc ggggaccggt cggtcgggct cgggatgcag ggccttgtcc
     3181 agggcctcgg tccagacctt ggcgagggca gccaggcggc ggccgttcgg ggacaggacg
     3241 gtgatgcgag gcccgccagg gatcgtcacc tccgcgaagc ccggatcgac cggggtggcg
     3301 atggcggcgc ctgcgaaggc gacgttccac ggcatacctg gctgggctcc gggagcaccg
     3361 ctcaaggtgg ccgtgagcgt ttctccttgg gcgacgctgc gcgggactgc gcgtggcgag
     3421 ctggcctgcc gatgcccgtt gaaccagaca tcgccgacgt gggtgcgggc gaagtcggag
     3481 gcgaggaagg ggatcatccc gccgatgtgg tcgctgtcga tgtgggtgac gacggcgacg
     3541 tcgatggcgg ggttgctggc tggcagccgg tccaggcggg ctttgaggag cggccaggtg
     3601 tccggtggcc cgccgtcgac gagcatccgc cagggtcggc ggcccggccg gaggcattcg
     3661 acgagcaggc agtcgccgag tcgggccggc agcatctcca cgcggatcgc ggtggccatg
     3721 gtggcagcgt cctccctcgc tgcacggcgc cgcaaccgat tctgcgaact gccctgtgga
     3781 tgagcgcgac cgggtgtcag ggggacggca tacggtgggt ccatggtcgc cgcactgtcc
     3841 ccgagccggg cctcggactt caagcagtgt ccgctgctct atcgcttccg ggtcatcgac
     3901 cggctgccgt cgccgacgtc cgctgcggcg gcgcgcggca ccctggtcca cgcggtgttg
     3961 gagcggttgt tcgacctgcc agcccacgag cggacccccg ccgcggccgc cgcgctggtg
     4021 gcgccgcaat gggagcggct ggtggccgag gagcccgagc tcgcgggcct gctggacgcc
     4081 gacgacgacg gcgcagccga cgcctggtat gcgcaggcgg tcgggctggt cgagcggtgg
     4141 ttcaccctcg aggacccgac ccggttggag ccggccgagc gcgagctcta tgtcgaggcg
     4201 gaggtggacg gcctggtcct gcgtggctat gtcgatcgtc tcgacgtggc ccctgacggg
     4261 tcgatgcgag tggtcgacta caagaccggg cgatccccgt cggagttgtt cgagcgttcg
     4321 tcgctgttcc agatgaagtt ctatgccctc gtgctgtggc ggttgcgcgg cgtggtgcct
     4381 cgtatgctcc aactggtcta cctcggcaac ggcgaggtag tgcgctacga gccggacgag
     4441 gccgacctcc tggccaccga gcgtggcatc aaggccctgt gggcggcgat cgagcgcgcc
     4501 gctgcgaccg gcgattggcg gccccgggtg tcacgcctgt gcgactggtg cgatcacaag
     4561 tcgctctgcc ccgaatgggg tgggactccc ccgccgctgc cgaccgacgc gcccgctcgg
     4621 gctctcgatc cggccgtcac cggtcgggta gtgccggccg acgactagtc ggtcagctgc
     4681 gggtcggtca gctgcgggtc ggtcagctgc gggtcggaag ggccgcgaag cccggcagcc
     4741 cgaccagatc cgttgggctc accccgacca gggtcgggat ctgcaccacg ccaggcatcc
     4801 ggggcaccgg cttcacgctg ggcaccacga tggtctgggc gccggccgcc gcggccgagc
     4861 gggctcccgg ctcggagtcc tcgatggcga tgcactcctc tggcgccagc cccagcccgg
     4921 ctgcacctcg gaggtagggc tcagggtgcg gtttgccgtg ggtgacgtct tcgcccgaga
     4981 cgatcaggtc gaaggcgcca ccgggcaagg cgtcgccgat ggcgtcggtg atcgggcggt
     5041 aggacatcgt caccagcgca gtcgggacgc cggcttcgcg tagcgcggtg agcagttccc
     5101 gcgccccggg gcgccacggc acctctgccc ggatccggcg gacgacgccg gtcaccagtc
     5161 ggtcgacgag ctcatcgatc ggcaggtcca cggggctgtg gtccttgatg tatgccgccg
     5221 agtcggtcag cgcgcgaccc accagagcga aggcatcggc ctcggtccac acgccgccga
     5281 agtcctcgac cagagcgcgt tcctcgagga cccagtaggc ctctgagtcg acgagagtgc
     5341 cgtcgaggtc ccacaacacg gccgctggca ggcgggcaga catcttggtg agaccccttc
     5401 gcaggtggtg gtgcgttcgc cgccgccgga gggtgacgag cccaccaagt ctaccgaccg
     5461 cgccgtgttg gtccgccggg cgaggcgcgg cggcataccg gctgggggcc gtggccctag
     5521 ggtggtgtcc tccgattcct gccctgcgag gtgactgtgc ctgtgctccg ttccatccca
     5581 gagttgcgcg agccggtgct cgtcgcggcc ttcgagggct ggaacgacgc cggtgaggcc
     5641 gcgtctgcgg cggccgccca cctcatcgag gtgtgggatg cgcagcaggt cggtgagctc
     5701 gacccggagg actactacga cttccaggtc aaccggccga agatcaccct cgtggatggt
     5761 cagcgtcgga tcacctggcc gacgacgcgg atcttcgtct gccagggcac gaccgggccg
     5821 catgac
//
LOCUS       DAORPW010000142         5798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3810_length_5798_cov_524.068083, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000142 DAORPW010000000
VERSION     DAORPW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3810_length_5798_cov_524.068083"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLB86_10185"
     CDS             <1..558
                     /locus_tag="PLB86_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HRC65546.1"
                     /translation="GNICRSPAGERLLRHSWPAAALTVTSAGTYGVVGHPVEPAMARL
                     LQADGVDVSGFAARRLTEGLIARAGLVLTMETGHRTSVVQTHPQALARTFTLREFAHL
                     LEVCRVAEPAAYLAALGTDPTTRLREFTRLAADARSRRLAPPCAEPDIDDPYGRGDEA
                     YQRAYAEIRAAVDVIVRHASRVDDR"
     gene            complement(684..1670)
                     /locus_tag="PLB86_10190"
     CDS             complement(684..1670)
                     /locus_tag="PLB86_10190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC65547.1"
                     /translation="MLESQALTAAVVIITYRRPHFVRTCLEHLAALTRAPEETYVIDA
                     SPDGLTRDVVADFPTVRYLHSDAGPGTMATSRAMALAATTCDVLVFLDDDANADPDWL
                     KQLLLPYADPTVAAVGGRARNGQPGEETEGLDQIGLLTADGKLLGFFAADPGHDVDVD
                     HLLGANMSVRREVLLALGGIRDNYPGTCLREESDMFLRVRQAGHRIVYTPSAVVTHVA
                     APYVHGRRFDLRYHYYGHRNHIVLLTSTIGTRDPRTRRYVASVLREDIAGGLKEGAGA
                     LVDSDLAPRARIRRAGGRVLRAGVAAAGLVTGGVAAGRIAVAARRSASTPAP"
     gene            complement(1684..2688)
                     /locus_tag="PLB86_10195"
     CDS             complement(1684..2688)
                     /locus_tag="PLB86_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65548.1"
                     /translation="MQNHNVGSTQGWRTAYLVLTHTDAAAVVRLVAAIRRSSPRCVVI
                     VAHDARVQPPPAFADEHVLVWCHGLATDWGSWELVEATLGAVAQARAFADPDFFVLVS
                     GQDYPARHLGTWEDEVRAAGGGWIGRPRPLRYRPTWGRRPGVGDDDLTRYSFRWWPLP
                     PAVARMGRRFPERARHGAARVRDAVLLRAAPVVAVRFVSRGRGAHLGLPRSRRALGGR
                     VFVKCDQWIAFDRDLAAVVLRDLAAGMPLRRLFERSIIPDESAIQTALHAVEPPTIPH
                     PVSFVRWLPSLDRPAVLTLADLPELQASRSPFCRKVSSGHSADLLAALDDLVTGAGAR
                     "
     gene            2735..3757
                     /locus_tag="PLB86_10200"
     CDS             2735..3757
                     /locus_tag="PLB86_10200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC65549.1"
                     /translation="MPAPGFTLVIPTYHRPDLVAEAVASALAQVVPFARIIVVCDGDQ
                     PAVGERLAGLPVDVVTVAHGGVAAARNHGLSLVDTSWVCFLDDDNLLHPAYLASVQEF
                     VSTRDAVQALNTYYWVFATDFLPGVDVVAHNLAECLAAAATETPVNDLSYLHIAGQSY
                     RLLLERLRGSVSGCAITTEVLRAAGGFPAGRTCAEDWTMFVNVARLTEWQVIEDRLVF
                     FRDHPLTNTRTGLSANGVDTVRAMHEFWTDPRLHEPCPVDLGDYRAAYRTTVRFALEA
                     ARVDGSSGRFAEIQRLAPVVLPRRVDRILARVPPRWWDRLRVARSLIGVPRREADARV
                     AAKSVG"
     gene            3776..4987
                     /locus_tag="PLB86_10205"
     CDS             3776..4987
                     /locus_tag="PLB86_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HRC65550.1"
                     /translation="MSAATAISVRGLGKTYRIAHASDRPTRATEAIVARLRHPLRRTT
                     FDRFEALTEVSFDVAAGTALGIIGRNGAGKSTLLKILTRITPPTRGQAELAGRVGSLL
                     EVGTGFHPELTGRENVFLNGTLLGMRRQEITRRFDEIVDFSGCGQFLDTPVKRYSSGM
                     YVRLAFAVAAHLETEILVIDEVLSVGDADFQRRSVQKMRDAATQGRTVLYVSHQLHTV
                     TALCSEALLLDRGTVSAYGAVEDVLATYRASVAEHAVVQRETRAQVPAGILHVVAATT
                     TRLTYLPAEPKRFTFTIQAEAAPDGPYFLSCHINDANGGVVAQCDSRLGGLWFHPDQP
                     QEVTLDLRQPWLKPGTYTVDVFLCRVGIVDVWDGAVTFDVEQELPYAVTGTAESFGRG
                     MVLADFDFGVR"
     gene            4987..>5798
                     /locus_tag="PLB86_10210"
     CDS             4987..>5798
                     /locus_tag="PLB86_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC65551.1"
                     /translation="MAPHTVIGPPGPLHLPRWSELWEAREVLWRFGQRDILLRYRQTL
                     IGVAWVILQPIVTAGVFALVFGRVAQLPTGGIPYFLFGYVGTLLWTVFASTVNRAAGS
                     LVSNQALVSKVFFPRLLVPLSTCVSVLVDFLVALVIGFGLLWWFGVWPGWAILTIPVW
                     VTLCVALAMGIGLAAAAIMVRYRDIAYVLPWAVQTLLYASPVAYSLEAVPDAVRPYLL
                     ANPLTWVLEGMRWAMLGSAAPPVWQIVGAVVVSGLVLFAGLLVFQRYEREFA"
BASE COUNT          831 a         2033 c         1997 g          937 t
ORIGIN      
        1 ggcaacatct gccgctcccc cgcgggtgaa cggttgctgc gccactcctg gcccgccgcc
       61 gcgctcacag tgaccagcgc aggcacgtat ggcgtcgtcg gacacccggt ggaaccagca
      121 atggcgcggc tgctacaggc cgacggggtg gacgtctcgg gcttcgcggc ccgccggctc
      181 acggagggcc tgatcgcccg cgccggcctc gtactcacca tggagaccgg acatcggact
      241 tcggtcgtcc agacccaccc ccaggcgttg gcccgcacct tcaccctgcg cgagttcgct
      301 cacctcctgg aggtatgccg cgtggccgag cccgcggcat acctcgccgc cctcgggacc
      361 gacccgacga ctcgactgcg cgagttcacc cggctggcgg ccgacgcgcg gtcgcgacgc
      421 ctggccccac cgtgtgccga gcccgacatc gacgacccct acggccgcgg ggacgaggcc
      481 taccaacggg cgtatgccga aatccgcgct gccgtggatg tcatcgttcg acacgcaagc
      541 cgggtcgacg accgatagtg tcgccgccgc gtccgacccg ccggggtgag ggatcaccgc
      601 ggtgttcggc tctgtcgcct atcgactgat cgagcacccg tgcttgcgct gggcccggtc
      661 ggtgccctcg cccaccgtgc tgactacggc gcgggagtcg atgccgaacg gcgggcggca
      721 accgcgatcc gaccggcggc gaccccaccc gtaaccaggc cggcggcggc gacaccggcg
      781 cgcagaaccc ggccacccgc ccgacggatc cgggcgcgcg gcgccaggtc gctgtcgaca
      841 agggctcccg cgccctcctt cagccccccg gcgatgtcct cgcgcaggac gctggcgacg
      901 tagcggcggg tccgcgggtc gcgcgtgccg atcgtcgagg tcagcagcac gatgtggttg
      961 cggtgtccgt agtagtggta gcgcaggtcg aagcgccgcc cgtgcacgta gggcgcggcg
     1021 acgtgcgtca ccaccgcgct gggcgtgtag acgatgcggt gaccggcctg gcgcacccgc
     1081 aggaacatgt cggactcctc gcgcaggcag gtgccggggt agttgtcccg gatgcctccc
     1141 agggccagca ggacctcccg ccgcaccgac atgttggcgc ccaacaggtg gtcgacgtcg
     1201 acgtcgtggc cggggtctgc ggcgaagaag cccaggagtt tgccgtcagc cgtcagcagc
     1261 ccgatctggt ccaggccctc ggtctcctcg cccggctgcc cgttgcgggc cctgccgccc
     1321 actgcggcga cggtcgggtc ggcatagggc agcaggagtt gcttgagcca gtcggggtcg
     1381 gcgttggcgt cgtcgtcgag gaacaccagg acgtcgcagg tcgtcgccgc cagcgccatg
     1441 gcccgcgagg tcgccatcgt ccccgggccg gcgtcgctgt gcaggtaacg gacggtgggg
     1501 aagtcggcca cgacgtcacg ggtcagcccg tccggcgacg cgtcgatgac gtaggtctcc
     1561 tcgggcgccc gggtcagcgc ggccaggtgt tccaggcagg tgcggacgaa gtgcggcctg
     1621 cggtaggtga tgatgacgac ggcggccgtc agggcttggc tttcaagcat gcttcgcagt
     1681 ctctcatctg gcgccggctc cggtcacgag gtcgtccaac gccgcgagca gatctgctga
     1741 gtgacccgac gacaccttcc ggcagaacgg cgagcgcgag gcctggagct ccggaaggtc
     1801 ggccaaggtg aggacggcgg gccgatcgag cgacggaagc caccgcacga aactcaccgg
     1861 gtgggggatc gtcggcggct ccaccgcgtg cagcgccgtc tggatcgccg actcgtccgg
     1921 gatgatggac cgctcgaaga ggcgacgcag cggcataccc gcggcaaggt cgcgaagcac
     1981 gacggcggcg aggtcgcggt cgaaggcgat ccattggtcg cacttgacga acacccgccc
     2041 gccgagcgcc cgccgcgatc ggggcagccc cagatgagcg ccccggccgc gcgagacgaa
     2101 ccggacggcc acgacgggag cggcccggag aaggaccgcg tcgcggaccc gggccgcgcc
     2161 gtggcgggct cgttccggga accgtcggcc catccgcgcc acggccgggg gaagcggcca
     2221 ccaccggaag gagtagcggg tcaggtcatc atcacccacg cccggtcggc gtccccaggt
     2281 tggccgatat cgaagagggc gcgggcgacc gatccaccca ccacccgctg cccggacctc
     2341 gtcctcccac gtcccgaggt gccgcgcggg gtagtcctgc cccgagacca gcacgaagaa
     2401 gtccgggtcg gcgaaggccc tcgcctgcgc caccgccccg agggtcgcct cgacgagctc
     2461 ccaggacccc cagtcggtcg ccagcccgtg gcaccacacg aggacgtgct cgtccgcgaa
     2521 cgccgggggt ggctgcacgc gggcgtcgtg cgccacgatg acgacgcacc gggggctcga
     2581 ccgacggatc gcggcgacca gccggacgac cgccgccgcg tcggtgtggg tgagcacgag
     2641 gtatgccgtg cgccacccct gcgtcgatcc gacgttatgg ttctgcacga ccgtcagtat
     2701 tcccgcgtcg cccccgcatg acatcatcag ggcaatgcct gctccaggat tcacgctcgt
     2761 catcccgacc tatcatcgcc cggatctggt ggccgaggca gtggcgagcg cactcgccca
     2821 ggtcgtgccc ttcgcacgga tcatcgtcgt ctgtgacggc gaccagcctg ccgtcgggga
     2881 gaggttggcc ggcctgcccg tcgatgtcgt cacggtggcg cacggtgggg tcgcggcagc
     2941 ccgcaaccac gggctctccc tggtcgacac gtcctgggtg tgcttcctcg acgatgacaa
     3001 cctgctccac ccggcctacc tcgcgtcggt ccaggagttc gtgtcgaccc gcgacgcggt
     3061 tcaagcactc aacacctact actgggtgtt cgccaccgac tttctccccg gcgtggacgt
     3121 cgtggcccac aatctggcgg agtgcctggc tgcggccgcc accgagacac cggtcaacga
     3181 tctgagttat ctgcatatcg ccggccagag ctaccgcctg ctgctcgaac ggctgcgcgg
     3241 ttcggtgagc ggctgcgcca tcaccaccga ggtcctgcgg gccgccgggg ggttcccggc
     3301 gggccgcacc tgcgccgagg actggaccat gttcgtcaac gtcgcccggc tgaccgaatg
     3361 gcaggtcatc gaggaccgcc ttgtcttctt ccgggaccac ccgttgacga acacccgaac
     3421 cgggttgtcc gccaacggtg tagacaccgt gcgggccatg catgagttct ggaccgaccc
     3481 gcgcctgcac gagccctgcc cggtcgacct cggcgactat cgcgcggcgt acagaacgac
     3541 ggtccggttc gcgctggagg cggcgcgggt ggacgggtcc agcgggcggt tcgccgagat
     3601 ccagcgactg gcgcctgtcg tgctcccccg ccgcgtcgac cggatcctcg cgcgcgtccc
     3661 gccgcgatgg tgggaccggt tgagggtggc gaggtcgctc atcggggtcc ctcgacgcga
     3721 ggccgatgct agggtcgcgg caaagagtgt ggggtgaagg gcgggtacgg gagctatgtc
     3781 ggcggctacg gccatcagcg tccgcggact gggcaagacc taccggatcg ctcacgcctc
     3841 ggaccggcct actcgcgcga ccgaggccat cgtggctcgg ctgcgccacc cgctgcgtcg
     3901 gaccacattt gatcgcttcg aggcgctcac cgaggtgagc ttcgacgtcg ccgccggcac
     3961 cgcgttgggc atcatcggcc gcaatggcgc cgggaagtcg acgctgctga agattctgac
     4021 gcggatcacg ccgcccacgc ggggccaggc cgagttagcc ggccgggtcg ggagcctcct
     4081 cgaggtaggg acggggttcc acccggagtt gaccgggcgg gagaacgtct ttctcaatgg
     4141 cacgctgctg ggaatgcggc gtcaggagat cacccggcgc ttcgacgaga tcgtcgactt
     4201 ctccggatgt gggcagttcc ttgatacgcc agtcaagcgg tactcctcag ggatgtatgt
     4261 ccgattggcg ttcgccgtcg ctgcccacct cgaaaccgaa atcctcgtca tcgacgaggt
     4321 gctcagcgtg ggcgacgccg acttccaacg gcgctcggtg cagaagatgc gcgacgccgc
     4381 gacccagggg cggaccgtcc tgtacgtcag ccaccaactg cacaccgtca cggccctgtg
     4441 ttccgaagca ctgctgttgg accgtggcac ggtgagcgct tacggggccg tcgaggacgt
     4501 gctagccacc tatcgggcgt cagtggccga gcatgccgtc gtccagcgag agacccgcgc
     4561 acaggtgcca gccgggatcc tgcacgtcgt cgcggcgacg acgacccgcc tgacctatct
     4621 gccagccgag cccaaacgat tcaccttcac gatccaggcc gaagcggcgc cggacgggcc
     4681 ctacttcctc tcctgccaca tcaatgacgc caacggcggc gtggtcgcgc agtgcgactc
     4741 ccggctgggc gggctgtggt tccaccccga ccaaccgcag gaggtgaccc ttgacctgcg
     4801 ccaaccctgg ctcaagcccg gaacctacac cgtcgacgtc ttcctgtgtc gggtggggat
     4861 cgttgatgtg tgggacggcg cggtcacctt tgatgtcgag caggaactgc cgtatgccgt
     4921 gacggggacc gcggaatcct tcggtcgggg catggtgttg gcggatttcg atttcggtgt
     4981 gcggtgatgg caccgcacac cgttatcggt ccccccgggc ccctgcacct gccacgctgg
     5041 tcagagttgt gggaggcccg cgaggtcctc tggaggttcg gtcagcggga catcctcctg
     5101 cgctatcgcc agacactcat cggggtggct tgggtcatcc tgcagccgat cgtcaccgcc
     5161 ggggtgttcg cgctggtctt cgggcgcgtg gcccaactcc ccacgggggg cattccctac
     5221 ttcctcttcg gttatgtcgg caccctgctc tggaccgtct tcgcgtcgac cgtgaaccga
     5281 gcggccggct cgcttgtcag caaccaggcc ctagtgtcca aggtgttctt cccgcggttg
     5341 ttggtgccgc tgtcgacctg cgtgtcggtt ctcgtggact tcctcgtcgc gctcgtgatc
     5401 ggcttcggac tgctgtggtg gttcggtgtg tggcctggct gggccatcct caccatcccc
     5461 gtgtgggtca ctctgtgcgt ggctctcgcg atggggattg ggctcgctgc agcggccatc
     5521 atggttcgct atcgcgatat cgcctacgtc ttgccgtggg ccgtccagac cctgttgtat
     5581 gcctcccccg tcgcgtattc cctcgaagcc gttcccgacg cggtccggcc ctacctcctg
     5641 gccaatcccc tcacctgggt gctcgaaggg atgcggtggg ccatgctcgg ctcggccgct
     5701 cctccggtct ggcagatcgt tggcgccgtc gtcgtcagcg gtctcgtcct gtttgccgga
     5761 ctgctcgtgt tccagcgcta cgagcgcgag ttcgcgga
//
LOCUS       DAORPW010000143         5778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3824_length_5778_cov_653.092085, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000143 DAORPW010000000
VERSION     DAORPW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3824_length_5778_cov_653.092085"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="PLB86_10215"
     CDS             <1..132
                     /locus_tag="PLB86_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HRC65552.1"
                     /translation="WLSVGGADFAPRADLASRAEQITVANRLYAQRGLQPWGCRSAA"
     gene            129..995
                     /gene="rsmA"
                     /locus_tag="PLB86_10220"
     CDS             129..995
                     /gene="rsmA"
                     /locus_tag="PLB86_10220"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRC65553.1"
                     /translation="MTSGAALLGAAQIRELAATLGLRPTKAWGQNFVVDANTVRRIVR
                     LAEVGPDDSVVEVGPGLGSLTLALLDSGARVSAVEVDPVLAAALPDTVARHAPERAAY
                     LTVVTADALQVHSLPDPQPTALVANLPYNVSVPVVLSFLERFPTLDRVLVMVQLEVAE
                     RLVAGPGSKAYGVPSVKAAWYAAGRLAGTVPRSVFWPVPNVDSGLVELRRRPVPTTTA
                     SRDEVFAVVDAAFAQRRKTLRAALSGWAGGAAAAETVLRAAGIDPGARGEALPVTAFA
                     AIAAARRDRVTP"
     gene            1006..1944
                     /locus_tag="PLB86_10225"
     CDS             1006..1944
                     /locus_tag="PLB86_10225"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRC65554.1"
                     /translation="MSTVSAPPRSVTVRVPAKVNCELRVGPLGADGYHELSTVFMAVG
                     LYDEVTVTRAERWSVTTTGPYAAHVPSGSDNLAMRAARLLARHMRVTRAPVAIRIDKN
                     IPVAGGMAGGSADAAGALVACEHLWGLRMDRDELMELAAELGSDVPFCLAGGIAIGSG
                     RGERLAPVLARGTYHWVFALSSQGLSTPEVFGEFDRLTTDISVPQPEPSPAMMTALRS
                     GDPEALGDALVNDLQEPAFSLRPALRAVLDAGLEFGALGGIISGSGPTVAFLTGSSEH
                     ALDLCVALTASGVVDQVTRAKGPVHGVQILPGPTRE"
     gene            1960..>3526
                     /locus_tag="PLB86_10230"
     CDS             1960..>3526
                     /locus_tag="PLB86_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRC65555.1"
                     /translation="MGILVSLERVTVVHGVAHVLDALTVGVLGGQRIGVVGRNGGGKS
                     TLLRVLAGRQEPDSGRVTSVGGLTVGLLAQDDRLDPAATVRHALVGDRPEHEWAGDAR
                     VREVLDGLLGGVGMLAVGGPDALVGPLSGGERRRLALAALLVADPDLLLLDEPTNHLD
                     VEGVAWLAEHLATCRTRPDTALVAVTHDRWFLDAMATLTWEVDSGAVHSYDGGYAAYV
                     LAKAERSRVAAVTAERRDNLLRKELAWLRRGAPARTTKPKFRIDAANALIENEPPPRD
                     GVELVRFATRRLGKDVVDLFDATVRYGERTLLDKVTWQLAPGERVGIVGVNGAGKSTL
                     LQAISGEVPLAGGRRKVGKTVSLAHLTQEVRELDELAAYRVIEAIEAVRHSIMLGDRE
                     VSASQLATRLGFTGQRQQTRVGDLSGGERRRLQILRLLMAEPNVLLLDEPTNDLDIET
                     LTSLEDVLDGWAGTLLVVSHDRYLLERVCDRQVALLGDGRVRDLPGGVAEYLTLRAAM
                     PPTTGYAVPPAASS"
     assembly_gap    3527..3626
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3627..3892
                     /locus_tag="PLB86_10235"
     CDS             <3627..3892
                     /locus_tag="PLB86_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65556.1"
                     /translation="ASSGALGGGSGDAGPTQIQQREARKEMARIERALTRLAEREDRV
                     HAQMAKEATDHLAVLRLNGDLRAIVDERDDLEMAWLAAAEIVG"
     gene            complement(3945..4469)
                     /locus_tag="PLB86_10240"
     CDS             complement(3945..4469)
                     /locus_tag="PLB86_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC65557.1"
                     /translation="MSARAPGEVGDRPPDDVDRIVAAWRRELPHLDVEPLLVLSRITR
                     LARRLDLARGVAFATHHLESWEFDVLSALRRAGRPYQLSPGQLVTETLVTSGTMTNRV
                     DRLEQRGLVRRGPDPGDRRGVKVRLTRVGREAVDAALSDLLAHERELLGELTPEEQAT
                     LAGMLRRLNAPLEP"
     gene            4615..>5778
                     /locus_tag="PLB86_10245"
     CDS             4615..>5778
                     /locus_tag="PLB86_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC65558.1"
                     /translation="MSAPAVLWGQGWVDADDSTLLALVRDGNAEAYAELWRRHLPAAY
                     GVAHRYRGRTSAEDVVAEASLRVYDLIRAGKGPLTNFRSYFLSAVRTIAVDHARGDLR
                     AVPTEDASLEAAAPATAAYDPSPTVDHALVRAAFARLAERDQQVLWQTAVEGTTPAAV
                     ADSMGLTANTVSVIALRARDSLRAKYLDAHADRAISRAQDAECAWVLGQMGRFVRGKL
                     PVRQRARVEEHLRSCRHAQGLAYEMAEVNRALPAVIVPLIFLAGSSLVGVWAGAAGAA
                     GGVGASGASVGPREEGKAAVAAALAANVGTKIAALGVAAVLATGYAAAAGVGGGGGGA
                     AGGSGNGGAGPVAAAPVVPGAVPAQPRTAAPSQEAAPSQTAVPSPTAVPSATSS"
BASE COUNT          760 a         2044 c         2011 g          863 t
ORIGIN      
        1 tggctgtccg tcggcggtgc cgacttcgcc ccgcgggccg acctggccag ccgtgcggag
       61 cagatcaccg tcgccaaccg cctctacgcc cagcgcgggc tgcagccctg gggctgccgc
      121 tcggccgcgt gacctccggg gcagcgctgc tgggtgctgc ccagatccgc gaactcgccg
      181 cgaccctcgg gctgcggccg accaaggcgt ggggacagaa cttcgtcgtc gacgccaaca
      241 ccgtgcgccg catcgtgcgc ctggccgagg tcggccctga cgacagcgtc gtcgaggtcg
      301 gccccggact cggctcactc accctcgccc tgctcgacag cggggcgagg gtgagcgccg
      361 tcgaggtcga cccggtcctc gcagccgcgt tgcccgacac ggtcgcgcga cacgcgccgg
      421 agcgggcggc atacctcacg gtcgtgaccg ccgacgctct gcaggtgcac tccctgcccg
      481 acccgcagcc gaccgcgctc gtggcgaacc tgccctacaa cgtgtcggtg ccggtcgtcc
      541 tgtccttcct cgaacgcttc ccgaccctcg accgggtgct tgtcatggtc cagttggagg
      601 ttgccgaacg gctcgtggcc gggccgggga gcaaggccta cggtgtgccg agcgtcaagg
      661 ccgcttggta tgccgcgggg cgcctggccg gcaccgtccc gcgaagcgtc ttctggccgg
      721 tgcccaacgt cgactccggt ctcgtcgagc ttcgccgcag gccggtccca acgacaaccg
      781 cgtcccgtga cgaggtcttc gccgtcgtcg acgccgcctt cgcccaacgt cgcaagaccc
      841 tccgtgccgc gttgtccggg tgggccggtg gcgccgccgc cgccgaaacc gtgctgcgag
      901 ccgccggtat cgaccccggt gcccgcggcg aggccctgcc cgtgacggcc ttcgccgcca
      961 ttgccgccgc tcgtcgagac agggtgaccc cctagggtgg tgcccgtgag taccgtcagc
     1021 gccccgcccc ggtcggtgac ggtgcgggtg cctgccaagg tcaactgcga gttgcgggtc
     1081 ggccctctgg gggcggacgg ctaccacgag ttgtcgacgg tcttcatggc ggtcggcctc
     1141 tacgacgagg tcacggtcac ccgcgccgaa cgctggtcgg tgacgaccac cggcccgtat
     1201 gccgcccacg tcccgtccgg cagcgacaac ctggcgatgc gcgcggctcg actgctcgcc
     1261 cgacacatgc gggtcacccg cgcgccggtg gcgatccgca tcgacaagaa catcccggtc
     1321 gcggggggca tggccggagg gtccgccgac gcagccggcg ctctcgtggc ttgtgagcac
     1381 ttgtggggcc tgcggatgga ccgcgacgag ttgatggagt tggccgccga gctcggcagc
     1441 gatgtcccgt tctgcctcgc cggtggcatc gccatcggct cggggcgcgg cgagcgtctc
     1501 gcgccggtcc tggctcgcgg gacttaccac tgggtgttcg cgctcagctc gcagggcctg
     1561 tccacccccg aggtcttcgg ggagttcgac cggctcacca cggatatttc ggtgccgcaa
     1621 cccgagccat caccggcgat gatgacggcc ctgcgctccg gtgacccgga ggccctcggg
     1681 gatgccctcg tcaacgacct ccaggagcca gccttctcgc tacgccccgc gcttcgggcg
     1741 gtcctcgacg ctggcctcga gttcggagcc ctcggcggga tcatcagcgg cagcggcccg
     1801 actgtcgcct tcctcaccgg gtcgtccgaa cacgccctgg acctgtgcgt cgcgctgacc
     1861 gcctcggggg tcgtcgatca ggtgacccgc gccaaggggc cggttcacgg cgtccagatc
     1921 cttcccggcc cgacgcgcga gtgagttgct gacacctgca tggggatcct cgtctcactt
     1981 gaacgcgtca ccgtcgtcca cggcgttgcg cacgtcctcg atgccctcac cgtcggcgtg
     2041 ctcggtggac agcggatcgg ggtcgtcggg cgcaacggtg gcggcaagtc gacgctgctg
     2101 cgggtgctcg caggccgtca ggagcccgac tccggacggg tcacctcggt cggcgggctc
     2161 accgtcggcc tgctggccca ggacgaccgg ctcgaccccg ccgcgaccgt ccggcacgcc
     2221 ctggtcggtg atcgtcccga gcacgagtgg gctggtgacg cccgggtccg ggaggtcctc
     2281 gacggtttgc tcggtggggt gggcatgctc gccgtcggcg gtccggatgc cctcgtcgga
     2341 cccctgtcgg gtggcgagcg acggcgtctc gcgctggccg cactgctcgt ggccgacccc
     2401 gacctgctgc tgctcgacga gcccaccaac catctcgacg tcgagggggt cgcctggctc
     2461 gctgagcacc tggccacctg ccgcacccgc cccgacacgg cgctcgtcgc cgtcacccac
     2521 gaccggtggt tcctcgacgc catggccacc ctcacctggg aggtcgacag cggcgccgtc
     2581 cacagctacg acggcggcta cgcggcatac gtgctggcca aggccgagcg ctcgcgcgtg
     2641 gcagcggtga cggccgagcg ccgcgacaac ctgctgcgca aggaactcgc ctggctgcgc
     2701 cgcggggcac ccgcccgcac gaccaagccg aagttccgca tcgatgccgc caacgccctc
     2761 atcgagaacg agccgccacc gcgcgacggg gtggaactcg tccgcttcgc gacccggcgc
     2821 ctgggcaagg acgtcgtcga cctgttcgac gcgacggtgc gctacggcga gcggaccctg
     2881 ctcgacaagg tgacctggca gttggctccc ggcgagcggg ttggcatcgt cggagtcaac
     2941 ggagccggga aatcgactct tctgcaagcg atttcgggcg aggtcccact cgctggcggt
     3001 cgtcgcaagg tcggcaagac ggtctccctg gcccacctca cccaggaggt gcgcgagctg
     3061 gacgagctcg cggcataccg ggtcatcgag gcgatcgaag cggtccggca cagcatcatg
     3121 ctcggtgacc gcgaggtgag cgcgagccaa ctggccactc gcctgggctt caccgggcag
     3181 cggcagcaga cccgtgtcgg cgacctctcc ggtggcgagc ggcggcggct gcagatcctg
     3241 cgactgctca tggccgagcc caacgtcctg ctcctcgacg agccgaccaa cgacctggac
     3301 atcgagaccc tcacctcgct cgaggacgtc ctcgacgggt gggcgggcac gctgctcgtc
     3361 gtgtcgcacg accgctacct gctcgagcgg gtctgcgacc ggcaggtcgc gctgctcggt
     3421 gacggccggg tccgcgacct gcccggcggg gtcgcggaat acctcaccct gcgtgccgcg
     3481 atgcccccca ccaccgggta tgccgtgccg cccgccgcgt cgtcggnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnccgc gtcgtcgggg gctttgggcg gaggctcggg
     3661 cgacgccggc ccgacccaga tccaacagcg cgaggcgcgc aaggagatgg cgcggatcga
     3721 gcgggcgttg acccggctgg ccgagcgcga ggatcgcgtg catgcccaga tggccaagga
     3781 ggcgaccgat cacctcgcgg tgctgcgcct caacggcgat cttcgggcga tcgtcgacga
     3841 acgtgacgac ctcgagatgg cctggctggc cgctgccgag atcgtcggct gaacagggtc
     3901 ggggcatgac gatgcgccgt gaccaggtgc acctcaggag tagctcaggg ctcgagcggg
     3961 gcgttgagcc gccgcagcat cccggcgagg gtggcctgct cctcgggggt caactcgccg
     4021 agcaactccc gctcgtgggc caggaggtcg gacagggcgg cgtcgaccgc ctcgcggccg
     4081 acccgggtga ggcgcacctt gacgccacgg cggtcaccgg gatcgggccc gcgccggaca
     4141 agtccgcgtt gctctagccg atcgacgcgg ttcgtcatcg ttcccgaggt gaccagggtt
     4201 tcggtgacga gttggccggg ggacagttga tagggccgtc cggcccggcg cagcgccgag
     4261 aggacgtcga actcccagct ttccaagtga tgcgtggcga aggcgacccc tcgggccagg
     4321 tccagccgtc gggccagtcg agtgatgcga gagagcacga ggaggggctc gacgtccagg
     4381 tgcggcaact cgcgccgcca agccgcgacg atacggtcga cgtcatcggg cggtcggtcc
     4441 ccgacctcgc ccggcgcacg tgcgctcacc tcacccaccc actcaatgta gcgagagcgt
     4501 caagagtctt gacttcgaga gatccagaac ttccgccgaa acgggtgtca tgggcacaat
     4561 ggttctgcgt ctgtcatatg caggggtcta gcttgaggaa gggaggggtc tgacatgtcc
     4621 gcgccagccg tactgtgggg gcagggatgg gttgacgctg atgacagcac cctcctggcc
     4681 ctggttcgtg atggcaacgc cgaggcgtat gccgagttgt ggcgccgcca cctcccggcg
     4741 gcctatggcg tcgcccaccg ctaccgcggg cggacctcgg ccgaggacgt cgtcgccgag
     4801 gccagccttc gggtctatga cctcatccgg gccggtaagg gcccgctgac caacttccgg
     4861 tcgtacttct tgtcggcagt gcgcaccatc gccgtcgacc atgcccgagg tgacctgcgt
     4921 gccgtcccga ccgaggacgc ttctctggag gcagcggctc cggccacggc ggcatacgac
     4981 ccgagcccca ccgtcgacca cgccctggtc cgcgcggcct tcgctcgact cgccgagcgt
     5041 gatcagcagg tcctctggca gaccgcagtc gagggcacca cgcctgccgc cgtcgccgac
     5101 tccatggggc tgaccgccaa caccgtcagc gtcatcgcgc tgcgggctcg cgacagcctt
     5161 cgtgcgaagt accttgacgc acatgccgat cgggcgatca gccgggccca agacgccgaa
     5221 tgcgcatggg ttctgggcca gatgggtcgc ttcgtccggg gcaagctccc ggtccgtcag
     5281 cgcgcccgcg tcgaagaaca cctgcgctcg tgtcggcacg cccagggcct ggcctacgag
     5341 atggccgagg tcaaccgcgc cctgcctgcg gtcatcgttc cgctgatctt cctggctggg
     5401 tcgtcactcg tcggcgtctg ggccggtgct gccggtgctg ccggtggcgt gggtgcgtcc
     5461 ggcgcctctg tggggccccg cgaggagggc aaggcggcgg tcgcggccgc gctcgccgcc
     5521 aacgtcggga ccaagatcgc tgcgttgggt gtggccgccg tgcttgccac ggggtatgcc
     5581 gcggcggcgg gcgtcggtgg cggcggaggt ggcgctgctg gagggtccgg caatggggga
     5641 gccggtcccg tcgctgctgc gccagtggtg cccggtgcgg tgcctgcgca gccgcgcacc
     5701 gccgcgccct cgcaagaagc ggcgccgagc cagaccgcgg tgccgtcgcc gacggcggtt
     5761 ccttctgcga catcgagc
//
LOCUS       DAORPW010000144         5732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3874_length_5732_cov_712.270389, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000144 DAORPW010000000
VERSION     DAORPW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3874_length_5732_cov_712.270389"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLB86_10250"
     CDS             complement(<1..587)
                     /locus_tag="PLB86_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC65559.1"
                     /translation="MSRYAGHVTSGGASDVRELRELIIRKCSVGEYDNNAYLLTCRAT
                     GEQLLVDAAADAPRLRAFVAEGTGSLGGIVTTHRHGDHVRALPEIAAWSGAPTASGAE
                     DAAALPLAPDRALVHGDVLRVGAVDLDVVHLRGHTEGGVALAYADPAGFTHLITGDSL
                     FPGGVGNTKMPGQSFEQLYADVTARIFDVYGDDTWV"
     gene            complement(589..1284)
                     /locus_tag="PLB86_10255"
     CDS             complement(589..1284)
                     /locus_tag="PLB86_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HRC65560.1"
                     /translation="MTTDTSWADVAAATAALLETAYGMREGDQAADSLCAGWTRGHVL
                     THVARNADAIGNLVTWAVTGVPTPMYASAEAREADIEAGAARSVADLVADLEASAARL
                     ADQTADLAPAHDAVEIELRGGWRIRASELAWRRLREVAYHHVDLDAGFGFADLPPDLT
                     SAFLADEVAGLRRHPQAPALTLHTTTGEEFTVGAGTAYVTGTPAALLGWLARGLTEGL
                     ATDGALPHLPKGS"
     gene            1357..2094
                     /locus_tag="PLB86_10260"
     CDS             1357..2094
                     /locus_tag="PLB86_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC65561.1"
                     /translation="MGRSPVVSLVESQSEGWAVWRVRMLGDFVNGFAFRDANGAVTLL
                     DVGLPWSTRRVLAALAAIDSGPSDVARIMVTHAHSDHVGAAASLAARTGRAVDVHEAD
                     AAYVAAGESPPMSGLGGLVSRVVPGFAAAPVGVRLVDGQLLGVAGGLRVLHTPGHSPG
                     HVSFLHEPSRLLITGDAIFNIRGPRWPVKAFCTDFQLNRQTARLLGDLDYTMAAFTHG
                     PELRDSPREAIRDFLRREASGAVGGGT"
     gene            2258..>3726
                     /locus_tag="PLB86_10265"
     CDS             2258..>3726
                     /locus_tag="PLB86_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HRC65562.1"
                     /translation="MAERQPGRHSRDTLIVRGAREHNLKDISVELPRDSLIVFTGLSG
                     SGKSSLAFDTIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNR
                     NPRSTVGTITEVYDYLRLLFARVGRPHCPICDEPITRQSPQQIVDRLLELPERTRFQI
                     LAPVVKARKGEFVDLFAELQTKGFARARVDGEVIALADPPKLEKQIKHTIEVVIDRLV
                     AKGDDAAAKRRLTDSVETALVLAGGILLVDFVDREEGEPDKERRFSERMACPNDHPLA
                     IDEVEPRTFSFNSPFGACPACTGLGTELEVDPELCIPDEDLSIAQGAVAPWGSGSGSA
                     DYFMRVVQAVADDLKFSLDTPWRALPERAKEALLFGQNYKVHVTYRNRYGRDRSYTTG
                     FEGVVPFVKRRHSETDSEWSRDRYEGYMREVPCPVCRGARLKPEVLAIRVGGQNISQV
                     CALAINECAEFLRSVDFTDRERQIAERVIKEIGARLGFLL"
     assembly_gap    3727..3826
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3827..5131
                     /locus_tag="PLB86_10270"
     CDS             <3827..5131
                     /locus_tag="PLB86_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC65563.1"
                     /translation="EDTIATADWVVDIGPGAGEHGGHVVHCGPVAGLLAHPDSLTGMY
                     LSGRKSIPVPDVRRPQVKGREVTVIAARENNLRDVTVSFPLANLVAVTGVSGSGKSTL
                     VNDILYTVLANKLNKARQVPGRHKSVTGLEHLDKVVHVDQSPIGRTPRSNPATYTGVF
                     DHMRRLFAETTEAKIRGYLPGRFSFNVKGGRCDNCSGDGTIKIEMNFLPDVYVPCEVC
                     HGARYNRETLDVHFKGKTIADVLDMPIEEAAEFFAAVPAIARHLTTLNDVGLGYVRLG
                     QPAPTLSGGEAQRVKLAAELQKRSSGRTVYVLDEPTTGLHFEDISKLLGVLQGLVDKG
                     NTVIVIEHNLDVIKSADWIVDLGPEGGSGGGRVVAEGTPEQVAAHPDSHTGRFLAPVL
                     AKAAAGMPSARPSVERASARPRKATAVASGTAKRAVRGRHTE"
     gene            5134..5730
                     /locus_tag="PLB86_10275"
     CDS             5134..5730
                     /locus_tag="PLB86_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRC65564.1"
                     /translation="MPEARRTVTGEDWYGREVQGERFADTDFVAVDFGDLRSVGAVFE
                     NCTFRRCRFNTSVHTDSAFTGCTFAYCTFFDARFVRCKLVGSTFTGCEFDLVQVDGGN
                     WGFVALAGAALGSTQWRGVRLTEADLDRVQAVGAVWRDLDLTGASLVGIDLRQADLRG
                     SSLWSLDPAAAQLSGAIVTPEQALVIAAALGLDVRVDD"
BASE COUNT          809 a         2007 c         1949 g          867 t
ORIGIN      
        1 acccacgtgt cgtcgccgta gacgtcgaag atccgcgccg tcacatcggc atacaactgc
       61 tcgaacgact gccccggcat cttggtgttg ccgaccccgc cggggaagag cgagtcgccg
      121 gtgatgaggt gcgtgaagcc cgccgggtcg gcatacgcca acgcgacccc gccctcggtg
      181 tgcccgcgca ggtggacgac gtcgaggtcg accgccccga cccgcagcac gtctccgtgg
      241 acgagcgccc gatcaggagc cagcggcagg gcagcggcgt cctcggcccc cgaggccgtg
      301 ggggcgcccg accaggccgc gatctccgga agtgcgcgca cgtggtcgcc gtgacggtgc
      361 gtggtgacga tcccgccgag gctgcccgtg ccctcggcga cgaaggcccg cagccggggc
      421 gcgtcggccg ccgcatcgac gagcagctgc tcgccggtgg cccgacaggt gagcaggtag
      481 gcgttgttgt cgtactcgcc gacgctgcac ttgcggatga tcaactcgcg cagctcgcgg
      541 acgtccgacg cgcctccgga ggtgacgtgc ccggcatacc tgctcatctc aggacccttt
      601 cggaaggtgg gggagcgcgc cgtccgtggc gagcccctcg gtcagcccgc gcgccagcca
      661 cccgagcagc gcggcggggg tgccagtgac gtatgccgtc ccggcaccga ccgtgaactc
      721 ctcgcccgtc gtcgtgtgca gggtgagcgc cggtgcctgc gggtgccgcc gcaacccggc
      781 cacctcgtcg gcgaggaagg ccgaggtgag gtctggtggc aggtcggcga acccgaaccc
      841 ggcatcgaga tccacgtggt ggtaggccac ctcgcgcagc cggcgccagg ccagctccga
      901 cgcacggatc cgccagccgc cgcgcaactc gatctcgacc gcgtcgtggg ctggggcgag
      961 gtccgcggtc tggtcggcga gtcgggccgc gctggcctcg agatcggcga ccaggtcggc
     1021 cacagaccga gctgcaccgg cctcgatgtc cgcctcgcgg gcctcggcgg acgcatacat
     1081 cggggtgggc acgccggtca cggcccaggt gacgaggttg ccgatggcgt cggcgttgcg
     1141 ggccacgtgg gtgagcacgt ggccacgcgt ccaccccgcg cacagcgagt cggccgcctg
     1201 gtcaccctcg cgcatcccgt atgccgtctc caacagggcc gcggtcgccg cggcgacgtc
     1261 ggcccatgag gtgtcggtgg tcatggcctg acgctacccg gcggatccgg gtccggcgct
     1321 gtcgcggcgc ggcggcgtcg gcctatggtg tgtggcatgg gtcgttcccc ggtcgtgtcc
     1381 ctggtcgagt cgcagtccga gggctgggcg gtgtggcggg tgcggatgct cggcgacttc
     1441 gtcaacgggt tcgcctttcg cgacgcgaac ggtgcagtga ccctgctcga tgtcggcctc
     1501 ccgtggtcga cgaggcgggt cctggcggct ctggccgcga tcgactccgg tccgtcggac
     1561 gtcgcccgga tcatggtcac ccacgcgcac tccgatcatg tgggcgcggc ggcctcgctg
     1621 gccgctcgca ccggccgggc cgtggacgtt cacgaggcgg acgcggcata cgtcgcggcg
     1681 ggggagtcac ccccgatgtc tgggctgggc gggctcgtgt cgcgggtcgt gccgggcttc
     1741 gccgccgcgc cggtgggggt ccggctggtc gacggccagc tgctcggcgt agccggcggc
     1801 ctgcgggtcc tgcacactcc tggccattcg ccggggcatg tgtcgttcct gcacgagccg
     1861 agtcggttgc tcatcacggg cgatgccatc ttcaacatcc gtgggccgcg ttggccggtc
     1921 aaggcgttct gcaccgactt ccaactcaat cggcagaccg ccagactcct gggcgacctc
     1981 gactacacga tggcggcctt cacccatggg ccggagctgc gcgacagccc ccgggaggcg
     2041 atccgggact tcctccgccg ggaagcgtcg ggggctgtcg gtgggggcac ctaggctgtc
     2101 ctgcgtgtca gtcgtgcccc cttcgcccac ccggaaacgc tccggatcca ccccgcgatc
     2161 cgccgcacct tcggcggtgc cgtccaccaa gtcgtccaca tcgtcgtcca ccaagccgga
     2221 cccggcgacg gccggcgggg ctgctgtcgc gcatctcgtg gcggaacgac agccgggccg
     2281 acacagccgc gacaccctga tcgttcgggg ggcccgcgag cacaacctca aggacatctc
     2341 ggtcgagttg ccccgcgaca gcctcatcgt cttcacgggc ctgtccggct cggggaagtc
     2401 gtcgctggcg ttcgacacga tcttcgccga ggggcagcgt cgttacgtcg agtccttgtc
     2461 ggcctatgcc cgccagttcc tcggccagat ggacaagccc gacgtcgact tcattgaggg
     2521 cctctccccg gccgtgtcga tcgaccagaa gtcgaccaac cgcaacccac gctcgacggt
     2581 ggggacgatc accgaggtct acgactacct ccgtctgttg ttcgcccggg tcggtcgccc
     2641 gcactgcccc atctgcgacg agccgatcac ccgccagagc ccccagcaga tcgtcgaccg
     2701 actgcttgag ctccccgagc gcacccgttt ccagatcctc gcgccggtcg tcaaggcccg
     2761 taagggcgag ttcgtcgacc tgttcgccga gttgcagacc aagggcttcg cccgggcccg
     2821 ggtcgacggt gaggtcatcg cgctggccga cccgcccaag ctcgagaagc agatcaagca
     2881 caccatcgag gtggtcatcg accggttggt cgccaagggt gacgacgcgg cggccaagcg
     2941 acggctcacc gactcggtgg agaccgccct cgtgctggcg ggcggcatac tcctcgtcga
     3001 cttcgtggac cgcgaggagg gcgagcccga caaggagcgg cgcttctccg agcggatggc
     3061 ctgccccaac gaccacccct tggccatcga cgaggtcgag ccccggacct tctcgttcaa
     3121 ctcgcccttc ggcgcctgcc cggcctgcac cggtctgggc accgagctcg aggtcgaccc
     3181 cgagttgtgc atccccgacg aggacctctc gatcgcgcag ggcgcggtgg ctccgtgggg
     3241 gagcggctcc ggctcggccg actacttcat gcgcgtcgtc caggcggtgg ccgacgacct
     3301 gaagttctcg ctcgacaccc cctggcgggc gctccccgaa cgggccaagg aggccctgct
     3361 cttcgggcag aactacaagg tccacgtcac gtatcgcaac cgctacggcc gcgaccggtc
     3421 ctacaccacc ggtttcgaag gggtcgtgcc gttcgtcaag cgtcggcact ccgagaccga
     3481 ctccgagtgg agccgggacc gctacgaggg ctacatgcgt gaagtgccct gccccgtatg
     3541 ccgcggtgcc cggctcaagc cggaagtgct cgccatccgg gtgggtgggc agaacatctc
     3601 gcaggtctgt gccctggcga tcaacgagtg cgccgagttc ctgcgctcgg tcgacttcac
     3661 cgaccgcgag cggcagatcg ccgagcgggt catcaaggag atcggggccc ggttggggtt
     3721 cctgctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngagg acaccatcgc
     3841 gacggccgac tgggtcgtcg acatcgggcc gggagcgggg gagcacggcg gccatgtcgt
     3901 gcactgcggg ccggtcgccg ggctgctggc ccaccccgac tcgctcaccg ggatgtatct
     3961 ctcgggtcgc aagtcgatcc cggtccccga cgtgcgtcgc ccgcaggtca agggccgcga
     4021 ggtgacggtg atcgccgccc gcgaaaacaa cctgcgcgac gtcaccgtgt cgttccccct
     4081 ggccaacctc gtggcggtca ccggggtgtc cggctcgggg aagtcgacgc tggtcaacga
     4141 catcctctac acggtgttgg ccaacaagct caacaaggcc cgccaggtcc cggggcggca
     4201 caagtcggtc accggcctcg agcatctcga caaggtcgtc cacgtcgacc agagcccgat
     4261 cggccggacc ccgcggtcca acccggccac ctacaccggg gtcttcgacc acatgcgccg
     4321 cctgttcgcc gaaaccaccg aggcgaagat ccgcggctac ctgccgggcc ggttctcgtt
     4381 caacgtcaag ggcgggcgct gcgacaactg ctcgggcgac ggcacgatca agatcgagat
     4441 gaacttcctg ccagacgtct atgtgccgtg cgaggtctgt cacggggcgc gctacaaccg
     4501 ggagaccctc gacgtccact tcaagggcaa gacgatcgcc gacgtcctcg acatgcccat
     4561 cgaggaggcc gccgagttct tcgcggcagt gccggcgatc gctcgccacc tgacgacgtt
     4621 gaacgatgtc gggctgggct atgtccggct cggccaaccc gcgccgacgc tctcgggtgg
     4681 tgaggcccag cgggtcaagc tcgccgccga gttgcagaag cgctccagcg gccgcaccgt
     4741 ctatgtcctc gacgagccga ccaccggcct gcacttcgag gacatcagca aactcctcgg
     4801 cgtgctccag ggcctggtcg acaagggcaa cacggtcatc gtcatcgagc acaacctcga
     4861 cgtcatcaag tcggccgact ggatcgtcga cctcggtccc gagggcggct ccggcggcgg
     4921 ccgcgtcgtc gccgaaggca cccccgagca ggtcgccgcg catccggact cccacacggg
     4981 tcgcttcctc gcccccgtgc tcgccaaggc cgcagcgggt atgccgtccg cgcgccccag
     5041 cgtcgagcgg gccagtgccc gaccccgcaa ggccacggcg gtggccagcg gaacggcgaa
     5101 gcgggcggtg cgcgggcggc ataccgaatg accgtgcccg aggcgcgccg gacggtcacg
     5161 ggggaggact ggtacggccg cgaggtgcag ggtgaacggt tcgccgacac ggacttcgtg
     5221 gcggtcgatt tcggcgatct gcggagcgtc ggggctgtct tcgagaactg cacgttccgg
     5281 cggtgccggt tcaacacctc ggtgcacacc gactcggcct tcaccggctg cacgttcgcc
     5341 tactgcacct tcttcgacgc ccggttcgtc cgctgcaaac tcgtcggatc gaccttcacc
     5401 gggtgcgagt tcgacctcgt ccaggtcgac ggtggcaact ggggtttcgt ggctctggca
     5461 ggcgcggccc tcgggtccac ccagtggcgc ggtgtccgcc tcaccgaggc cgacctcgac
     5521 cgggtgcagg cggtcggcgc cgtgtggcgc gacctcgacc tcacgggtgc ctcgctggtc
     5581 gggatcgacc tgcgtcaggc agacttgcgc ggaagttcgc tctggtcgct cgatccggct
     5641 gccgcacagc tgagtggagc gatcgtgacg cctgagcagg cgctcgtcat cgccgccgca
     5701 ctcgggctgg acgttcgcgt cgacgactga gg
//
LOCUS       DAORPW010000145         5707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3898_length_5707_cov_783.916667, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000145 DAORPW010000000
VERSION     DAORPW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3898_length_5707_cov_783.916667"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLB86_10280"
     CDS             <1..988
                     /locus_tag="PLB86_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC65565.1"
                     /translation="RGRLYPYEGNYSTYLEKKAERLQIQGRKDVKLAKRLRDELEWVR
                     SNAKGRQTKSKARLARYEEMAAEADRTRKLDFEELAIPPGPRLGSQVIEVRKLRKGFG
                     DRVLIDNLSFTLPRNGIVGVIGPNGVGKTTLFKTIVGLEAPDSGEVKVGETVQISYVD
                     QGRSGLDPAKTLWEVVSDGLDFIKVGQVEIPSRAYVSQFGFKGPDQQKPAGVLSGGER
                     NRLNLALTLKQGGNLLLLDEPSNDLDVETLGSLENALLEFPGCAVVISHDRWFLDRVA
                     THILAYEGDEEDPAKWYWFEGNFEAYEANKVERLGIEATRPHRVTYRKLTRD"
     gene            complement(1019..1597)
                     /locus_tag="PLB86_10285"
     CDS             complement(1019..1597)
                     /locus_tag="PLB86_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HRC65566.1"
                     /translation="MTGPVSPTGPPTTAGHSVLVVPIPALETLVAARHAHYDRDYVSA
                     DPTFAHAHVTILGPWLTQADLADPVRANAALTAVRDIASATRGFDVDFAHVDTFPNGI
                     IHLVPEPAEPFAALTAAVYREFPAYPPYAGQFGVVQPHLTLDAVGAGVTEAVVRGWVA
                     DRLPLRVRADRLHVSWYEAGRCQTLAVFPFGR"
     gene            complement(1594..2388)
                     /locus_tag="PLB86_10290"
     CDS             complement(1594..2388)
                     /locus_tag="PLB86_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC65567.1"
                     /translation="MPTSATPPPAAQPPARRRTGRFGEKEWEVYDAWTPVTLRGDVTV
                     VLVHGGLWQAAYDRAHLRPLGAALARDGWPVASVEYTRVGMPRGGWPGTAESVLAAID
                     AVAADPELPDRLVAVGHSAGGHLAVWAASNGRCPALTGVVSLAGVLDLRLADTDDLGS
                     GAVRALLGGEPTECPQAWVEADPMAARLDVPAVLLHGRSDDLVPSEVSASYLRSRGPL
                     DAPCRLELVPACDHFGIIDPRHAAYERVLAAIGEVAGTSPAGEPRS"
     gene            2500..3414
                     /locus_tag="PLB86_10295"
     CDS             2500..3414
                     /locus_tag="PLB86_10295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRC65568.1"
                     /translation="MRLGTQISYFTWPDAPASIGPTFGRIARDADAAGMDSLWVMDHF
                     FQISMIGPAELDMLEGYTALAFAAGQTQRIQLGTMVTGVTYRYPGVLAKTVTTLDVLS
                     GGRAWLGIGAAWNEEEHLGLGVPYPALAERFERLEETLQIALQMWRGDESPYAGQHYQ
                     LTRPLNVPQSLTRPHPPILIGGGGEKKTLRLVAQYADACNIFDMGPAAITAKYDVLRG
                     HCDALGRNYADIEKTVLSRLTLGDGSPDATGTPTVSVAEGVERMGRLAEIGTDHVILG
                     FANVATPGAFDQVAELVRQVAPIVPAGR"
     gene            3433..4377
                     /locus_tag="PLB86_10300"
     CDS             3433..4377
                     /locus_tag="PLB86_10300"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRC65569.1"
                     /translation="MLSVLAITIPIYACVAVGHVAVRRGLLSRSDMRVLTTFVISISL
                     PCMLFLAIGTRPVRDILNPTYLAAYALGSVLAYAVGYGYGRLRGAGPAGRAFDGLGMS
                     GSNSGFVGYPLLLLTLPDVAGLVLGLSMIVENLLVLPLMFLLAEGRAQGHDSRAKVLA
                     TYATRLTRNPLLIGLVAGLIVSLSGVHLPEAVLRTADLFSRAGTAVALFAIGGLLATF
                     PRGAELRRVLALSLGKLLLHPLLVAGALLVLVGLGLPALDPALRIGLLVTGALPIMST
                     MPVLAHPYGESEPIAGVLLVTTGVSFLTLSGLLGILTG"
     gene            complement(4346..4996)
                     /locus_tag="PLB86_10305"
     CDS             complement(4346..4996)
                     /locus_tag="PLB86_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC65570.1"
                     /translation="MLSTVSTTPAAVTPASETVQRLFSAAAEAFAAKGFHATTTRDIA
                     SRAGLSPAGVYVHFASKEDLLFHLSRDGHEEALALVTEAVAGADSPAEALAATMSRFS
                     AWHAENYAIARVVQHEFPHLSDAHRAEVLALRKRIDQAVRDVLAAGKQSGDFLLDDIP
                     DTALALLSIIVDVARWYTPALHRTSAQIGATNAALGLRLVGYRGGQINQSGSRAIH"
     gene            5067..>5707
                     /locus_tag="PLB86_10310"
     CDS             5067..>5707
                     /locus_tag="PLB86_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC65571.1"
                     /translation="MPRNLYSEEHEDFRASVREFVERSLKPRVEEMISLKSIPRDIWL
                     EAGKQGFFGMDIPEEFGGAGAGDYRFNAVLAEEWAHFNAATAACFGIHADVCPPYIVD
                     LGTEEQKQRWLPGIASGEKIIAIGMTEPSGGSDLAALKTTAVRADDGSGDWIINGSKT
                     FITNGYQCDLCIVAARTDPTKGPKGITLFMVERGMDGFVNGRKLDKVGQDESDT"
BASE COUNT          896 a         1943 c         1977 g          891 t
ORIGIN      
        1 ccgcggccgg ctgtatccct atgagggcaa ctactcgacc tacctggaga agaaggccga
       61 gcgcctgcag atccagggcc gcaaggacgt caagctcgcc aagcggctgc gcgacgagct
      121 ggagtgggtc cgctccaacg ccaagggtcg ccagaccaag agcaaggccc gactggctcg
      181 atacgaggag atggcggccg aggccgaccg gacccgcaag ctggacttcg aggagcttgc
      241 catcccgccg ggcccgcgcc tggggtcgca ggtcatcgag gtccgcaaac ttcgcaaggg
      301 tttcggcgac cgcgtgctca tcgacaacct gtcgtttacg ttgccccgca acgggattgt
      361 cggcgtcatc ggccccaacg gcgtcggcaa gacgacgttg ttcaagacca tcgtcggtct
      421 ggaggcgccg gactctggcg aggtcaaggt cggcgagacg gtgcagatct cctatgtcga
      481 ccagggccgc tccggcctcg acccggccaa gaccctctgg gaggtcgtct ccgacgggct
      541 ggacttcatc aaggtcggcc aggtcgagat cccgtcgcgg gcctatgtct cgcagttcgg
      601 gttcaagggc cccgaccagc agaagcctgc cggggtgctg tccggtggcg agcgcaaccg
      661 gctcaacctc gcgctcaccc tcaagcaggg cggcaacctc ctgctcctcg acgagccgtc
      721 caacgacctc gacgtcgaga cgttgggctc gctggagaac gcccttctgg aattccccgg
      781 ctgcgctgtc gtcatcagcc acgaccggtg gttcctcgac cgggtcgcga cccacatcct
      841 ggcctatgaa ggcgacgagg aagacccggc caagtggtat tggttcgagg ggaacttcga
      901 ggcctacgaa gccaacaagg tcgagcgact gggcatcgag gcgactcgtc cgcaccgggt
      961 cacctaccgc aagctcaccc gcgactgacg agtcggcagc cctgcgcgtc gggtgcggtc
     1021 agcgcccgaa cgggaacact gccagggtct gacaccggcc cgcttcgtac caggacacgt
     1081 gcagtcgatc ggcgcgcacc cgcagcggta gccggtcggc gacccaccct cgaacgacgg
     1141 cttcggtgac tcccgcgccc acggcgtcga gcgtgaggtg cggctgcacc acgccgaact
     1201 gaccggcata cggggggtat gccgggaact cgcgatacac agcggcggtg agcgcggcga
     1261 acggttcggc tggctcggga acgaggtgga tgatcccgtt cgggaacgtg tccacgtgcg
     1321 cgaagtcgac gtcgaatccc cttgtcgccg aagcgatgtc gcggactgcg gtgagcgccg
     1381 cgttcgcccg gaccgggtcg gccaggtcgg cctgggtcag ccaggggccg aggatcgtca
     1441 cgtgggcatg cgcgaaggtc ggatcagccg agacgtagtc gcggtcgtag tgcgcgtgcc
     1501 gggccgccac gagtgtttcc agtgcaggaa tgggcacgac gagcacgctg tgtccggccg
     1561 tggtgggcgg tccggtgggg gagaccgggc cagtcatgac ctcggctctc ctgccgggga
     1621 ggtgccagcc acctccccga tcgcggccag cacccgctcg tatgccgcgt gcctcgggtc
     1681 gatgatcccg aagtggtcgc aggccgggac gagctccaac cggcaagggg catccagggg
     1741 accccgggag cgcaggtagg acgccgagac ctccgagggg accaggtcgt cgctgcgccc
     1801 gtgcaggagg accgctggga cgtccaggcg ggcggccatc ggatcggcct cgacccacgc
     1861 ctgcgggcac tcagtgggct ccccacccag caaggcccgg accgcaccag agccgagatc
     1921 gtcggtgtcg gccaggcgca ggtcgagcac cccggcaagc gacaccacac cggtcagggc
     1981 agggcaacgg ccattgctcg ccgcccacac cgcgaggtgg ccgccagccg aatgtcccac
     2041 cgcgacaagc cggtcgggca gctcggggtc agcagcgacg gcgtcgatgg cggccaggac
     2101 ggactcggcc gtgcccggcc agccgccgcg cggcataccg acgcgggtgt attccacgga
     2161 ggcgacgggc catccgtccc gcgccagcgc cgctcccagc gggcgcaggt gggcccggtc
     2221 gtatgccgcc tgccacaggc cgccgtggac caggacgacc gtcacgtcac cgcgcagcgt
     2281 gactggggtc caggcgtcgt agacctccca ttccttctcc ccgaaacggc cggttcggcg
     2341 gcgggcggga ggctgggccg cgggaggcgg ggtcgcagag gtgggcacgc caccatccca
     2401 ccatcctttc cccgtgcctc tttccccgtg acctaagggg ccggccggtc ttcgggcaac
     2461 ccgttatgcg cgcctgccgg ttacccgcag actgtcccca tgcgccttgg tacccagatc
     2521 tcctacttca cctggcccga cgccccggcg tcgatcggcc ccaccttcgg ccggatcgcg
     2581 cgggacgccg atgccgcggg gatggactcg ctgtgggtga tggaccactt cttccagatc
     2641 tcgatgatcg ggccggccga gctcgacatg ctcgagggct acaccgcgct cgcgttcgcg
     2701 gccggtcaaa cccagcggat ccagctcggc acgatggtca ccggcgtgac ctatcgctac
     2761 cccggcgtgc tcgccaagac cgtcacgacg ctggacgtgc tctccggagg gcgcgcgtgg
     2821 ctgggcatcg gcgcggcctg gaacgaggag gaacacctcg gcctcggcgt cccgtatccg
     2881 gcgctcgccg agcgcttcga aaggcttgag gagaccctgc agatcgccct gcagatgtgg
     2941 cgaggtgacg agagcccgta tgccggtcag cactatcaac tcacccggcc cctcaacgtc
     3001 ccgcagtcgc tgacccgacc gcacccgccg atcctcatcg gcggcggtgg cgagaagaag
     3061 acgttgcgac tggtcgcgca gtacgccgac gcctgcaaca tcttcgacat gggcccggcg
     3121 gcgatcaccg cgaagtacga cgtgctgcgc ggacactgcg acgcgctcgg acgcaattac
     3181 gccgacatcg agaagaccgt gctcagccgg ctgaccctcg gggacggttc gccggatgcc
     3241 accgggacgc cgacggtgtc ggtcgccgag ggggtcgagc ggatgggccg actcgccgag
     3301 atcggtaccg accacgtcat cctcgggttc gccaacgtcg ccacacctgg ggcgttcgac
     3361 caggtggccg agctcgtccg gcaggtggcc ccgatcgtcc cggcgggccg ctgactcaat
     3421 agcctggccg ggatgctttc ggtcctggcc atcaccatcc cgatctatgc ctgtgtcgcg
     3481 gtcgggcacg tggccgtgcg gcggggtctg ctgagccgct cggacatgcg agtccttacg
     3541 acgttcgtca tctcgatctc gttgccgtgc atgctgttcc tcgccatcgg gacccgcccg
     3601 gtcagagaca tcctcaatcc gacgtacctc gcggcatacg cgctgggatc ggtcctggcc
     3661 tacgccgtgg gctacgggta tgggcggctg cgcggcgccg gtccggcggg gagggccttc
     3721 gacgggctgg gcatgtccgg gagtaacagc ggtttcgtgg gctatccgct gctgcttctc
     3781 acgctgcccg acgtcgctgg gctggtcctg gggttgagca tgatcgtcga gaacctcctc
     3841 gtgctgccgc tgatgtttct gctcgccgaa gggcgcgctc agggacacga ttcccgagcc
     3901 aaggtgttgg ccacttacgc gacccgattg acccgcaacc cgttgctcat cggcctggtc
     3961 gccgggctga tcgtctcgct ctccggggtg cacctgcctg aggcggtcct gcgcacggct
     4021 gacctgttct ccagggctgg aacggccgtc gcgctgttcg ccatcggagg gctgttggcg
     4081 accttcccgc ggggcgccga acttcgtcgg gtgctcgcac tgagcctagg caaactcctc
     4141 ctgcatccgc tgctcgtggc gggggccctg ctcgtcctcg tgggtttggg actgccggcg
     4201 ctcgacccgg ccctgcgcat cgggttgctc gtcaccgggg cgcttccgat catgtcgacc
     4261 atgccggtgc tggcccaccc gtacggcgag agcgaaccca tcgccggggt gctgctggtg
     4321 acgaccggcg tgtccttcct caccctcagt ggattgctcg ggatcctgac tggttgatct
     4381 ggccgccgcg gtagccgacc aggcgcagcc cgagggcggc gttcgtcgca ccgatctgag
     4441 ccgaggttcg gtgcaacgcg ggggtgtacc accgggcgac gtcgacgatg atcgacagga
     4501 gcgcgagggc cgtgtcgggg atgtcgtcca acaggaagtc gccggactgc ttgcctgccg
     4561 cgagaacgtc gcgcacggcc tggtcgatcc gcttgcgcag ggccaggacc tcggctcggt
     4621 gggcgtcgga caggtgcggg aactcgtgct gaacgacgcg ggcgatcgcg tagttctcgg
     4681 cgtgccaggc gctgaagcgg gacatcgtcg cggcgagcgc ctcggcgggg gagtcggcgc
     4741 cagcaaccgc ctcggtgacc agcgcgaggg cttcctcgtg gccatctcgg gagaggtgga
     4801 agaggagatc ttccttggac gcgaagtgca cgtagacccc ggcgggagac aaaccggcgc
     4861 gcgaggcgat gtcgcgcgtc gtggtggcgt ggaagccctt ggcggcgaag gcttccgccg
     4921 cagcggagaa cagtcgctgc acggtctcgc tggctggggt caccgccgca ggagtggtcg
     4981 acacagtgga cagcatgccg cacctatgtc atgctaagca agcgcttact tacgtccatc
     5041 gtcggacccg catctggagg tcactcatgc cgcgcaacct gtattccgag gagcacgagg
     5101 acttccgggc cagcgtccgg gagttcgtgg agcgctcgct caagccccgc gtcgaggaaa
     5161 tgatcagcct caagtcgatt ccccgcgaca tctggctcga ggccggcaag cagggcttct
     5221 tcggcatgga tatccctgag gagttcggtg gggccggcgc cggtgactac cgcttcaacg
     5281 ccgtgctcgc cgaggagtgg gcccacttca acgcggccac ggccgcgtgc ttcggcatcc
     5341 acgccgatgt ctgcccgccc tacatcgtcg acctgggcac cgaggagcag aagcagcgct
     5401 ggctccccgg catcgcgtcc ggcgagaaga tcatcgccat cggcatgact gagccgtccg
     5461 gcgggtcgga tctcgccgca cttaagacga cggcggtgag ggccgacgac ggctccggcg
     5521 actggatcat caacggctcc aagaccttca tcaccaacgg ctaccagtgt gacctgtgca
     5581 tcgtcgcggc ccgcaccgac ccgaccaagg gcccgaaggg catcacgttg ttcatggtcg
     5641 agcgcggcat ggacggcttc gtcaacggcc gcaagctcga caaggtcggc caggacgagt
     5701 ccgacac
//
LOCUS       DAORPW010000146         5667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3936_length_5667_cov_869.509622, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000146 DAORPW010000000
VERSION     DAORPW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3936_length_5667_cov_869.509622"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            460..756
                     /locus_tag="PLB86_10315"
     CDS             460..756
                     /locus_tag="PLB86_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65572.1"
                     /translation="MATPRLRPVAPGERAKKDAPKTIAQAAESGSARDLLVAMRSRIA
                     KQVDDLNTPARDLAALTKRLIEVVRDIEAIDAAHEQDGDDDAEVEDGEFDASAV"
     gene            710..2134
                     /locus_tag="PLB86_10320"
     CDS             710..2134
                     /locus_tag="PLB86_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65573.1"
                     /translation="MTQRSKTESLTPRLSEYARTFTYPDGIVRTVWPRVEAKGRELGL
                     GFDWWQQQAGTVILGYGADGRYVATVGGVGMSIPRQVGKTYFVLAMIVILCILFPGLQ
                     VVWTAHHLRTSTKTFTTLRGICRRKKVAPLVRSIRSANGEQQVEFSNGSTIMFGARAQ
                     GFGRGFDEIDIEVFDEAQILDTKALEDMVAATNQARHIHGALLLFMGTPPRKSDPSSE
                     FRLRRSEAQAGEAHDAIWIEIGADPESDPNDQSQFPLMNPSFPLRTPLESLQRLRKNL
                     KDPDSWNREGRGIWDPEVTGGALSHARWLTLADPHAERGSDVVFGVDLTGDRDVWIAV
                     AWTRDDGATQVMLANEGRPVAAYSAVSECKRLTGEWGGTVASSAFGDDFEREGVPFEP
                     VNGTEFAAACGLVEDAIKDSSVRHGNQAALNDGVKAAKWRPQTTSGERAFVLRDAPEV
                     GPVAAVARALWALNNGKGGGFNVW"
     gene            2124..2321
                     /locus_tag="PLB86_10325"
     CDS             2124..2321
                     /locus_tag="PLB86_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65574.1"
                     /translation="MSGDAVERIGIVAVLLGVASVSVAAFLVGVVLGLVVAGALALAL
                     GVTLVGWAHSATVEPTPEPTP"
     gene            2360..3544
                     /locus_tag="PLB86_10330"
     CDS             2360..3544
                     /locus_tag="PLB86_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRC65575.1"
                     /translation="MPLTADNLMTLIGGDKPVSSGAVVTPRSSLGVSAVWRAVNLLSG
                     TIASLPLHVYRPRPDGGRERVLSGRAYGLVDDPHRDLTRFEWWEFAIASMLLWGNAYF
                     WRRNASRLGPLAWLDPIDPSRVKVGRASDGTKVFTVNDVPYTEDEIFHLPGFGYDGTS
                     GLSPITIARQGIGLALSSEEYAARLFGSGSLATGILQTDQRLTQEQADTIQSRWAAKR
                     SGLGSAHGTIVLDNGAKFFQLTIPPVDAQFLESRSFQVSEVARMFGIPPHMLMDTEKS
                     TSWGTGIEQQSIVWVVYTLRPWLSRLEQRITRLIKPQSVYARWSVEGLLRGDSAQRSA
                     FYQALWGIGVLSTNEVRALEDMEPVEGGDVRYRPLNMGVLGTADAADNPDPATPQEAT
                     SA"
     gene            3537..4292
                     /locus_tag="PLB86_10335"
     CDS             3537..4292
                     /locus_tag="PLB86_10335"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HRC65576.1"
                     /translation="MRNSPFRFRGSSMPAAGVRASILSSESDGGVATLRLYDPIDSWG
                     GDWGVSAKEFAAALAVIPRDTTEIRLHINSPGGEVFEAVAILNQLRAHPAKVVAVVDG
                     LAASAASFLATGADETIMGQNTQLMIHDAWGLCVGNAGDMRGLADLLDRLSNNIADVY
                     AAKAGGTTEDWRTAMLAESWYSADEAVAAGLADSVAGDEPQPSAAFDLSTFKFAGRAE
                     APGPLTPVASGRRLSSAARATDLQAKRFIPLPQ"
     gene            4395..5639
                     /locus_tag="PLB86_10340"
     CDS             4395..5639
                     /locus_tag="PLB86_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HRC65577.1"
                     /translation="MSTSNLREQRANIWEQMKALSDRATAENRDLSAEETVSYSKMEA
                     DLDALGNRIEREEKLAARAPEFDRGASPASGPPPVVDEPHGDADYQAAFAAYIRNEPM
                     SYEQQKALQGGFDSSIKNAAGVGTGAAGGYTVPPEFRDKFIETQKWYGPMLQEAEVIY
                     TDSGANLQWPTNDDTANVGAILAENSQVTEQDVTFGTASIDSYMYTSKLVRASLQFLQ
                     DNAINADSWLAERLGVRVGRILNQHATTGTGTAQPDGLVTGATVGATSTGSFATTGGI
                     SYDSLIDLVESLDPAYGGGPGLKFMAHQTARKAIRKIKDSQGRPLWEPSLQVGQPDTL
                     LGYGLVLNNDMPAMAQSSKSLGFGNIRAAYVIRIVKGLTVLRLTERYADFLQVGFLGF
                     ERADGTLQDSSAFKIHQTTATA"
BASE COUNT          923 a         1940 c         1912 g          892 t
ORIGIN      
        1 gatgtgttcg ggggctgaac tgaggtattg tctgccggga agagtcatgc ccaaagccag
       61 cccggcctag cgccgggctt cctgctgtca ggggggtgcg tggctggcaa gtacggccat
      121 gagcaccgca aggtcagagc ccaactgcag cgtctcatcg acgcagggca aggcttgtgt
      181 gctgagccca tctgcctgat gccgagccgc gagataccgc caggctccgg ccccgacgac
      241 tggcacgtct gccacgaccc gagcggcacc cagatcatcg gaccagggca cgcacggtgc
      301 aacgagagcg aagcggccaa gcgtggcaac gcgatgcgac gaccagcgga cgtgacgacc
      361 ctcacctggt aggggggtcg gaatcttaag cgggacggcc ccacgacccc gcagccgaca
      421 aaaatctctc tccgggtttt tccaccaaag ggggtccccg tggcgactcc tcgccttcgc
      481 cccgtcgctc ccggagagcg ggccaagaag gacgcgccga agaccatcgc gcaggctgcc
      541 gagtccggct ctgctcgtga cttgctcgtg gcgatgcgct cacggatcgc gaagcaggtc
      601 gatgacctga acactccggc gcgcgacctc gccgcgctga ccaagcgact gattgaggtc
      661 gtccgagaca tcgaggcaat cgacgccgcc cacgagcagg acggggacga tgacgcagag
      721 gtcgaagacg gagagtttga cgcctcggct gtctgagtac gcgcggacgt tcacctaccc
      781 agatggaata gtccgcaccg tctggcctcg tgttgaggcg aagggccgtg agctcgggct
      841 tggcttcgac tggtggcagc agcaggcggg gacggtcatc ctcggctatg gcgcggatgg
      901 gcgctacgta gcgacggtcg gcggggtcgg tatgtcgatc cctcggcagg tcggcaagac
      961 gtacttcgtc ctggccatga tcgtcatcct ctgcatcctg ttccctgggc tacaggtcgt
     1021 ttggaccgcg caccatctgc gcacgtcgac caagacgttc acgacgttgc gcggcatctg
     1081 ccggcgcaag aaggtcgcgc cgctggtgcg gtcgatccgg tcggcaaacg gcgagcagca
     1141 ggtcgagttc tcgaacggct cgacgatcat gttcggcgcg cgagcccagg gctttgggcg
     1201 tggcttcgac gagatcgaca tcgaggtatt cgatgaggcc cagatcctcg acaccaaggc
     1261 gcttgaggac atggtcgccg cgaccaacca ggcgcgccac atccacggtg cgctgctgct
     1321 gttcatgggc accccgccgc gcaagtctga cccgtcgagc gagttccggt tgcggcgctc
     1381 ggaggcgcag gctggcgagg cgcacgacgc gatctggatc gagatcggcg cagaccccga
     1441 gtctgacccg aacgaccagt cgcagttccc gctcatgaat ccgtcgttcc cgctgcgcac
     1501 tccgctggag tcgttgcagc ggttgcggaa gaacctcaag gaccccgact cgtggaaccg
     1561 cgagggtcgg ggcatctggg acccggaggt cacgggcggc gctctctcgc acgcccgttg
     1621 gctcaccctc gctgacccgc acgcggagcg cggaagtgac gtcgtgttcg gcgtcgacct
     1681 gaccggcgac cgtgacgtgt ggattgccgt ggcgtggacg cgcgatgacg gggccactca
     1741 ggtgatgctc gccaacgagg ggcgtccggt cgcggcatac agcgcggtct ccgagtgcaa
     1801 gcggctgact ggcgagtggg gcggcaccgt cgcgtcttcg gcattcggcg atgacttcga
     1861 gcgcgagggc gtcccgttcg agccggtcaa cggcaccgag ttcgcggcgg cgtgcgggct
     1921 cgttgaggac gcgatcaagg actcgtcggt gcggcacggc aatcaggccg cgctcaacga
     1981 cggggtgaag gccgccaagt ggcggcccca aacgacaagc ggggagcggg cgttcgtcct
     2041 gcgcgacgcg ccagaggttg gcccggtcgc cgcggtggcg cgggcgctgt gggctctcaa
     2101 caacgggaag ggaggtggct tcaatgtctg gtgacgccgt cgagcgcatc ggaatcgtgg
     2161 ccgtcctcct gggagtggcc tccgtctcgg tcgccgcgtt cctcgtggga gtcgtgctcg
     2221 gcctcgtcgt tgccggcgcg cttgctctgg cgctcggcgt gacgctggtc ggttgggcgc
     2281 acagtgccac cgttgagccg actccggagc cgacgccgtg atcctccccc gcgtcaaggc
     2341 ctcgatcgag tctccgactg tgcccctgac tgccgacaac ctcatgaccc tgatcggcgg
     2401 ggacaagccg gtctcgtctg gtgccgttgt cacgccgcgg tcgagcctgg gcgtctctgc
     2461 tgtgtggcgc gccgtcaacc tcctctcggg caccatcgcc tcactccccc tgcacgtcta
     2521 ccggcctcgg cccgatgggg gtcgtgagcg ggtcctgtcc ggccgtgcgt acggcctggt
     2581 ggacgaccca caccgcgacc tcactcgatt cgagtggtgg gagttcgcta tcgcttcgat
     2641 gctcctgtgg gggaacgcct atttctggcg gcgcaacgcg agccgtctcg gcccgctcgc
     2701 gtggctcgac ccgatcgacc cgtccagggt caaggttggg cgcgcatcgg acgggaccaa
     2761 ggtgttcacg gtcaatgacg tgccgtacac cgaggacgag atcttccatc ttcccggctt
     2821 cggctacgac ggcaccagcg gcctctcgcc catcaccatc gctcgtcagg gcatcggcct
     2881 cgcgctgtcg tccgaggagt atgccgcgcg cctgttcggg tccgggtcgc tcgcgaccgg
     2941 catcctgcag acagaccagc gactcactca ggagcaggcc gacacgatcc agtcgcggtg
     3001 ggctgcgaag cgctccggcc ttgggtcggc gcacggaacc atcgtgctcg acaacggggc
     3061 caagttcttc cagctcacga tcccgcccgt cgacgcacag ttcctcgagt ctcggtcctt
     3121 ccaggtgtcc gaggtcgcgc gtatgttcgg catcccgccg cacatgctca tggacaccga
     3181 gaagtcgacg tcgtggggaa cgggcatcga gcagcagtcg atcgtttggg tggtctacac
     3241 cctgcggccg tggctgtctc ggttggagca gcggatcacc cgcctcatca agcctcagtc
     3301 ggtgtatgcc cgctggtccg ttgagggcct gctgcgtggt gactccgccc agcggtcggc
     3361 gttctatcag gcgctatggg ggattggcgt gctgagcacc aacgaggtgc gcgcgcttga
     3421 ggacatggag ccagtggagg gcggagacgt gcgataccgc ccactcaaca tgggcgtcct
     3481 cggcactgcc gacgccgccg acaatcccga cccggccacg ccccaggagg cgaccagtgc
     3541 gtaattcccc gttccgcttc cgtggctcct cgatgccagc ggccggcgtt cgcgcgtcca
     3601 tcctctcgtc cgagtcggac ggtggcgtgg cgaccctgcg cctgtatgac cccatcgatt
     3661 cgtggggtgg cgattggggt gtctcggcca aggagttcgc cgccgcgctc gccgtgatcc
     3721 cgcgggacac gaccgagatc cggctgcaca tcaactcccc tggcggcgag gtcttcgagg
     3781 cggtcgcgat cctcaaccaa ctgcgcgccc acccggcgaa ggtggttgcc gtcgtggacg
     3841 gcctcgccgc ctctgccgcc tcgttcctcg cgacgggcgc ggacgaaacg atcatgggtc
     3901 agaacacgca gttgatgatc cacgacgcct gggggctctg cgtcggtaac gcgggcgaca
     3961 tgcgcggcct ggctgacctg ctcgaccggc tgagcaacaa catcgccgac gtctacgccg
     4021 cgaaggcggg cgggaccacc gaggactggc gcacggccat gctcgccgag tcgtggtact
     4081 ccgctgatga ggcggtcgcc gctggcctcg ccgactcggt cgctggcgac gagccccaac
     4141 cgtctgccgc gttcgacctg tccacgttca agttcgccgg ccgcgcggaa gcgcctggac
     4201 ctctgacccc ggtcgcatcc ggtcgccggc tgagcagcgc cgcgcgcgcc accgacctgc
     4261 aggccaagcg gttcatcccg ctcccgcagt aaccactcca ccccagcaac ccaccgggac
     4321 agcccgagtg ggtcactccg ccgcgtccgc gtgcggggga accccatccg cgacgcggaa
     4381 ggaaggcaag gaccatgagc accagcaacc tgcgtgagca gcgcgccaac atctgggagc
     4441 agatgaaggc actgtccgac cgggcaacgg cggagaaccg cgacctgtcg gccgaggaga
     4501 ccgtctccta cagcaagatg gaggccgacc tcgacgcgct cggcaaccgg attgagcgcg
     4561 aggagaagct cgccgcccga gccccggagt tcgaccgcgg cgcttccccc gcatccggcc
     4621 cgccccccgt cgtcgacgag ccccacggcg acgccgacta ccaggccgcg ttcgcggcct
     4681 acatccgcaa cgagccgatg tcctacgagc agcagaaggc gctgcagggc ggcttcgact
     4741 ccagcatcaa gaacgcggct ggcgtcggaa cgggagcggc gggcgggtac accgttcccc
     4801 cggagttccg ggacaagttc atcgagaccc agaagtggta cggcccgatg ctgcaggagg
     4861 ccgaggtcat ctacaccgac agcggtgcga acctgcagtg gccgaccaac gacgacactg
     4921 cgaatgtcgg cgcgatcctc gccgagaact cgcaggtcac ggagcaggat gtcaccttcg
     4981 gcacggcgtc gatcgactcc tacatgtaca cgagcaagct ggtgcgcgcc tccctgcagt
     5041 tcttgcagga caacgccatc aacgccgact cgtggctggc ggagcggctc ggcgtccgcg
     5101 tcggtcgcat cctcaaccag cacgccacca ccggcacggg caccgcccag ccggacggcc
     5161 tcgtcacggg cgcgacggtc ggggccacga gcaccgggtc cttcgcgacc acgggtggca
     5221 tctcctacga cagcctcatc gacctggtcg agtctctcga cccggcatac ggcggcgggc
     5281 cgggcctcaa gttcatggcg caccagacgg cgcgcaaggc gatccgcaag atcaaggact
     5341 cgcagggtcg gcccctgtgg gagccgtcgc tgcaggtcgg tcagccggac accctgctcg
     5401 gctacggcct cgtcctgaac aacgacatgc ccgcgatggc gcagtcgtcc aagtcgctgg
     5461 gattcggcaa catccgcgcg gcctacgtga tccgcatcgt caagggcctc acggtcctgc
     5521 gcctcacgga gcgctacgcc gacttcctgc aggtcgggtt cctcggcttc gagcgggcgg
     5581 acggcaccct gcaggacagc agcgccttca agatccacca gaccacggcc acggcctgag
     5641 caacgacaac gccgcagggc gggccga
//
LOCUS       DAORPW010000147         5663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3940_length_5663_cov_842.067582, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000147 DAORPW010000000
VERSION     DAORPW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3940_length_5663_cov_842.067582"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PLB86_10345"
     CDS             <1..312
                     /locus_tag="PLB86_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRC65578.1"
                     /translation="LTDVAASKVKSLLAQEGRDDLRLRIGVQPGGCSGLIYQLYFDER
                     TLDGDALRDFDGVEVVVDRMSAPYLDGATIDFADTIEKQGFTIDNPNAGSSCACGDSF
                     S"
     gene            complement(410..946)
                     /locus_tag="PLB86_10350"
     CDS             complement(410..946)
                     /locus_tag="PLB86_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65579.1"
                     /translation="MSAYEWFRIRRTMLAYDYWLDVEGVTGRARKDLRAELVGNLTDA
                     TAAVGYPAARRSLGSVRTLATAAAPDRDSRPRWVVGSYAAIVVFAIGAFAALGLITAW
                     ADGVMAAGGGQGLWIGHPFFLPWVEITVRQGETLQILATFNPWSVVVPPLVAFLIGSR
                     IWRLATRQSSPTPAAQVR"
     gene            complement(948..1295)
                     /locus_tag="PLB86_10355"
     CDS             complement(948..1295)
                     /locus_tag="PLB86_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRC65580.1"
                     /translation="MTTHDPQMIKGVLGLLLLQLLDEGDGYGYGIVTRLQENGFEDLA
                     EGTVYPALARLEAAGHLDSYLLRSAAGPARKYYRITAGGRTELIRARRDWDALVERVR
                     LLTPTQLTAQTGA"
     gene            1398..2384
                     /locus_tag="PLB86_10360"
     CDS             1398..2384
                     /locus_tag="PLB86_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRC65581.1"
                     /translation="MRVAVTGSIATDHLMTFGGKFADSLVVEQLDKISLSFLADDLQI
                     RRGGIAANIAFGMANLGQHPVLIGSVGKDFDDYRSWLERHGVDCQSIHVSGKSHTARF
                     VCTTDVDMAQIATFYPGAMTEARGIELKPVADRVGGIQLVLIGPNDPVAMQRHTDECR
                     TRGIPFAADPSQQLAWLDGRGIRDLIEGAAYLFTNEYEAHMIAQKAGWDERDLADAVS
                     VQVTTKGKDGATIRQKGHDPIHVDVVPGVQPLDPTGVGDAFRGGFLTGLAAGLDHRRC
                     AELGSTLAAYVIETVGTQEYSLTTSAFVQRIHEAYGADAATAIRSHIRCPKP"
     gene            2408..3082
                     /gene="aat"
                     /locus_tag="PLB86_10365"
     CDS             2408..3082
                     /gene="aat"
                     /locus_tag="PLB86_10365"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRC65582.1"
                     /translation="MPLDPGPSRYAFDIERADAGEDLVGAGADLEPATILAAYRAGAF
                     PMGLGRHGAGPMGWWSPDPRGVLRPEDLRVSRSLRRSCARLQVTVDTAFDAVVRGCAD
                     PDREGRWITRPVTRAYTRLHALGWAHSVEVWSGDQLVGGLYGIAIGGLFAGESMFHRV
                     TDASKVALVALRELITADGDPRRIIDVQWVTPHLASLGVREVPRAAYLGLLREALKAP
                     LPAPWR"
     assembly_gap    3175..3274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3275..4284)
                     /locus_tag="PLB86_10370"
     CDS             complement(<3275..4284)
                     /locus_tag="PLB86_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRC65583.1"
                     /translation="MAAAWEAGWADPRRLHAEGRRARRLLDEARSVLAQGLGVAPETL
                     SFTPGGPTALRLGLEGLRYAARRTGARLVAATVEHSAILLPGRHAAAAAGDPGQFGPV
                     PVDALGRVDLAAWATALDSPGTAVAALQSANGEVGTRQPLAAAHEECRRRGIPLLVDA
                     TAGLGRDATPESYDVLVGDARSFAGPPGIGLLVLPPRTRWRLPGPPTEVEGGRVEIEP
                     VLPLALGAAEAWAQTVAARDADARAARALVDTIRAAAAAIPDTEVVGDPLDRLPHVVT
                     CSFLLADGEALVTELDRRGIAVASGSACTSSTLEPSHVLAAMGALTHGNIRVTLPLES
                     VSP"
     assembly_gap    4369..4468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4603..5514
                     /gene="coxB"
                     /locus_tag="PLB86_10375"
     CDS             4603..5514
                     /gene="coxB"
                     /locus_tag="PLB86_10375"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRC65584.1"
                     /translation="MRKHADTSARVTRRGLTIAGLAASATLLLSSCANVNSSASSGWL
                     PEGTAGAHVTSETGRITTLWVGTWIAGLAVGALVWGLAIWCMIAYRRKKDDPELPPQL
                     RYNVPIELLYTVVPVLMIAVLFYYTARDEAALMDVSTKPDYTINVVGKQWSWDFNYVD
                     DNVYSSGVHSELNPNGGVTGTPQPVLYLPVNKRVEFVLNARDVIHSFWVPAFLVKLDM
                     IPGKTNRLQITPTQVGEFQGKCAELCGAYHSQMLFKVKVVSQEDFDAEMAKLKSLGQE
                     GQLPNTLNREPLETKDLKMVPTPTGSK"
BASE COUNT          860 a         1880 c         1896 g          827 t
ORIGIN      
        1 ctcaccgacg tcgcggcctc gaaggtcaag agcctgttgg cccaggaggg ccgcgacgac
       61 ctgcgccttc gcatcggcgt ccagcccggt ggctgctcag gtctcatcta ccagctctac
      121 ttcgacgagc gcacccttga tggcgacgca ctgcgcgact tcgacggggt cgaggtcgtc
      181 gtcgaccgga tgagcgcccc ttacctcgac ggcgcgacga tcgacttcgc cgacaccatc
      241 gagaagcagg gcttcaccat cgacaacccc aacgcaggca gctcgtgcgc atgcggtgac
      301 agcttcagct gacctgctcc cgcagagcga acgaaggccc ccggaccatt gaggtctcgg
      361 gggccttcgc gtacgccgtg acgcgaggtc ccgacacgac gtcccgacgt cagcgcacct
      421 gggcagcagg tgtgggggag gactgccggg tggccagtcg ccagatccgc gacccgatga
      481 ggaaggcgac gagcgggggg accaccacgc tccaggggtt gaaggtggcg aggatctgca
      541 gcgtctcgcc ctggcggacc gtgatctcga cccaggggag gaagaaggga tgcccgatcc
      601 acagcccctg accgccgcct gccgccatga caccgtcagc ccacgccgtg atcaggccga
      661 gcgcggcaaa ggctccgatc gcgaagacga cgatcgcggc atacgacccg acgacccagc
      721 ggggccggct gtcccggtcc ggcgctgcgg cagtggcgag ggtgcggaca ctgcccagcg
      781 accgccgagc ggccgggtaa ccgaccgcgg cggtggcgtc cgtgagattc ccgacgagtt
      841 ccgcgcgcag gtccttgcgg gctcgacctg tcacgccttc gacatcgagc cagtagtcgt
      901 aggcgagcat cgtgcggcgg atccggaacc actcgtatgc ggacatgtca ggcccctgtc
      961 tgtgcggtga gttgagtcgg cgtgaggagt cggacgcgct cgacgagtgc gtcccagtcg
     1021 cgacgggcgc ggatcagctc cgtgcggcct ccggcggtga tccggtagta cttgcgggcc
     1081 ggcccagcgg cggagcgcag gaggtaggaa tcgaggtggc cggccgcctc caaccgggcc
     1141 agcgcgggat acacggtgcc ttcggcgagg tcctcgaagc cgttctcctg caatcgggtg
     1201 acgatgccgt agccgtagcc gtcaccctca tcgagcagtt gcagcaggag cagcccgagc
     1261 acccctttaa tcatctgtgg gtcgtgcgtt gtcatggcta tgagagtatg actagtacct
     1321 agacatgtca agtagtggtg attcctcagt tccggccctg cgatccaggg ggtatgccgt
     1381 ccactagcgt tgcgggcatg cgcgtcgccg tgacggggtc catcgccacc gaccacctga
     1441 tgaccttcgg ggggaagttc gccgactccc ttgtcgtcga acagctggac aagatctcgt
     1501 tgtccttcct cgccgacgac ctgcagatcc gtcgcggggg catcgccgcc aacatcgcct
     1561 tcggcatggc caacctcggc cagcaccccg tgctgatcgg ctcggtcggc aaggacttcg
     1621 acgactatcg atcctggctg gagcgccacg gggtggactg ccagtcgatc cacgtgtccg
     1681 ggaagtccca caccgcccgg ttcgtgtgca cgaccgatgt cgacatggct cagatcgcga
     1741 cgttctatcc cggcgcgatg accgaggccc gtggcatcga gctcaagccg gtggccgatc
     1801 gggtcggcgg gatccagttg gtcctcatcg gccccaacga cccggtggcg atgcaacgcc
     1861 ataccgacga gtgccgcacg cgcggcatac cttttgcggc cgatccgtcc cagcaactgg
     1921 cgtggttgga cgggcgcggg atccgcgacc tcatcgaggg cgccgcctac ctgttcacca
     1981 acgagtacga agcgcacatg atcgcccaga aggccggctg ggacgagcgc gacctcgccg
     2041 atgccgtcag cgtccaggtg acgacgaagg gcaaggacgg cgcgacgatc cgccagaagg
     2101 gccacgaccc gatccacgtc gatgtcgtcc cgggcgtcca gccgctggac ccgacggggg
     2161 tcggtgacgc cttccgcggc ggcttcctca ctgggttggc tgcgggcctg gaccaccgtc
     2221 ggtgcgccga gctcggctcg acgctcgcgg catacgtcat cgagacggtc ggcacccagg
     2281 agtattcgct gacgaccagc gccttcgtcc agcgcatcca cgaggcctac ggagccgacg
     2341 ccgccacggc gatccgctcc cacatccgct gccccaaacc ctgacggacc cgggtcagcc
     2401 atccgccatg ccgctcgatc cggggccttc gcgctatgcc ttcgacatcg agcgcgcaga
     2461 cgcgggcgag gatctcgtcg gcgccggtgc cgatcttgag ccggcgacca tcctcgctgc
     2521 ctaccgagcc ggggccttcc ccatggggct gggtcggcat ggtgcggggc cgatggggtg
     2581 gtggtcgccc gatccgcgcg gcgtgctccg acccgaggac ctgagggtca gccgctcgct
     2641 gcggcgctcc tgcgctcggc tgcaggtgac ggtcgacacg gccttcgatg cggtcgtccg
     2701 cggctgtgcc gaccccgacc gcgagggccg gtggatcacc cgtccggtga cccgggccta
     2761 cacccgactg catgcgctcg ggtgggcgca ctcggtggag gtctggtccg gggatcaact
     2821 cgtcggcggg ctctacggca tcgcgatcgg tgggttgttc gcgggggagt cgatgttcca
     2881 ccgggtgacc gacgcctcca aggtcgcgct ggtcgccttg cgcgagctca tcacggccga
     2941 cggcgatcct cggcggatca tcgacgtcca gtgggtcacg cctcacctgg cgtcgttggg
     3001 cgtgcgggag gtgccgcggg cggcatacct gggtctgctg cgggaggccc tgaaggcgcc
     3061 gcttccggcg ccgtggcgct gactccgcgc acgaggtatg ccgtgtgctc gccctcgacg
     3121 cgcacgccga gcaggagcag cccccgcatc cggacgaagg ccgcaacgtc ggtgnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcgac accgactcga ggggcaaggt
     3301 cacccggatg ttgccgtggg tgagggcgcc catcgccgcg agcacgtggc tcggctcaag
     3361 ggtgctcgat gtgcacgccg agccggacgc gaccgcgatg ccgcgccgat cgagttcggt
     3421 gacgagggct tcgccgtcgg ccaagaggaa cgaacacgtc acgacatggg ggagtcggtc
     3481 gagcggatct ccgaccacct cggtgtcagg gatcgctgcc gccgcggccc ggatcgtgtc
     3541 gacgagggcg cgagccgcgc gggcgtcggc gtcgcgtgcg gcgaccgtct gcgcccaggc
     3601 ttccgcggca cccagcgcca aggggagcac cggctcgatt tcgacccggc cgccctccac
     3661 ctcggtgggc gggcccggca ggcgccagcg ggtccgcggc gggaggacca gcagaccgat
     3721 acccggggga ccggcgaacg agcgggcatc cccgaccagg acgtcgtagg actcgggggt
     3781 ggcgtcccgg cccaggcccg ccgtggcatc gacaagcagg ggtatgccgc gccgccggca
     3841 ctcctcgtgc gcggccgcga ggggctggcg ggtgccgacc tcgccgttgg cgctctgcag
     3901 cgcggccacg gccgtgccgg gggagtccag ggccgtcgcc cacgcggcga ggtcgacccg
     3961 cccgagggca tcgaccggga ccggaccgaa ctgtccaggg tcgcccgcgg ccgcggcggc
     4021 gtgtcgcccg ggcagcagga tcgccgagtg ttcgaccgtg gcggcgacca gtcgcgctcc
     4081 ggtgcggcgg gcggcatacc gcagaccctc cagcccgagg cgcaaggcgg tcggcccacc
     4141 gggggtgaag ctcagcgtct cgggggcgac tccgaggccc tgcgcgagca cggatctggc
     4201 ctcatcaagc agtcgccggg cccggcggcc ctcggcgtgc aggcgccgcg gatcggccca
     4261 cccagcctcc caggccgcgg ccatcgtctc gcgtgccgcc gggtggacgg gaccctgcgc
     4321 ggcatcgagg agcgcccggc gggagtgcct gaaaggaggc tccgttgann nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnag gctccgttga gggcccgaat gagggatacg
     4501 ccacgtcgag aggttatcgg cacccctgcc gtgatccgtc gattccatcg cggtagggtg
     4561 gccccacaca cgagcagtca ctctgtattc ggaaggtgcc cgttgcgtaa gcacgcagac
     4621 acctccgctc gcgtcacgcg gcggggattg accattgccg ggctggcggc ctccgccacg
     4681 ctcctccttt ccagttgtgc caatgtcaac agcagcgcct ccagtgggtg gctgccggag
     4741 ggcacggctg gagcgcacgt cacgagcgag acgggtcgga tcaccacgct gtgggtgggc
     4801 acctggatcg ccggcctcgc ggtcggcgcc ctcgtctggg gcttggcgat ctggtgcatg
     4861 atcgcctacc ggcgcaagaa ggacgatccg gaactgccgc cccagttgcg ctacaacgtg
     4921 ccgatcgagt tgctctatac ggtcgtgccg gtcctcatga tcgccgtgtt gttctactac
     4981 acggcgcgtg acgaggccgc gctcatggac gtctcgacca agccggacta cacgatcaat
     5041 gtcgtcggca agcagtggag ctgggacttc aactacgtcg atgacaacgt ctactccagt
     5101 ggcgtccact ccgagctcaa ccccaacggg ggagtgacgg ggacgccgca gccggtgctc
     5161 tacctgccgg tcaacaagcg cgtcgagttc gtcctcaacg cccgcgacgt catccactcg
     5221 ttctgggtgc ccgcgttcct ggtcaagctc gacatgatcc ccggcaagac caaccggttg
     5281 cagatcaccc cgacccaggt tggcgagttc cagggcaagt gcgccgagtt gtgcggtgct
     5341 taccactcgc agatgctctt caaggtcaag gtcgtctcgc aggaggactt cgacgccgag
     5401 atggccaagc tcaagagcct cggccaggag ggtcagctgc ccaacacgct caaccgtgag
     5461 cccctcgaaa ccaaggacct caagatggtc ccgaccccga ccgggagcaa gtgatgactg
     5521 cgacgatcga gcgccccgag gccgcggccc gggtgcagca cccgcggcca cggccggggc
     5581 agacgttcta cagctggatc acgacgaccg atcacaagaa gatcggcaac ctctacttca
     5641 tcacgtcgtt cgtcttcttc ctg
//
LOCUS       DAORPW010000148         5645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3962_length_5645_cov_860.064401, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000148 DAORPW010000000
VERSION     DAORPW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3962_length_5645_cov_860.064401"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLB86_10380"
     CDS             <1..462
                     /locus_tag="PLB86_10380"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRC65585.1"
                     /translation="PLNDLLADFKRKRQQMAIVIDEYGGTEGIITLEDILEEIVGDYE
                     DEFTRGTRRVKKLTGSAYEIDASMRISDLVDLVSFPFPEDDDYVTLAGLFYKAFGGVP
                     SVGDVVTLESGRLTVLEMDNHRITLVKFEDTAHQADGSVGLVESTSAPDPV"
     gene            498..1703
                     /locus_tag="PLB86_10385"
     CDS             498..1703
                     /locus_tag="PLB86_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010080005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC65586.1"
                     /translation="MPSAPVPASPPVRLSARGAVEPFHVMEVIKAASLRQASHGDVIA
                     LCAGQPSTPAPVAARVAAVRALEGEVLGYTEATGIRPLREAIARHYGDSYGLAVDPPQ
                     VVVTTGSSAAFTALFLAAFDVGDTVAMSRPGYPAYRNTLQALGARVVELDCGPDTRFQ
                     PTVAMLEALPEPPAGLVVASPSNPTGTIIDPDELAAIARWCEAHGTLLVSDEIYHGIS
                     FGRRCASAWESSRSAVLVGSLSKYFSMTGWRVGWMVLPPELVRPVEVLIGNLNICPPA
                     VSQAAGVAAFSASAREELDGHVRRYAVNRDLLLRRLPEIGVAKLAPPDGAFYLYFDVS
                     HLTDDSTRWCQEVLDATGVALTPGVDFAPATPGGDPALDGRAFVRLCFAGTTADLDDA
                     MDRLTAYLT"
     gene            1843..3306
                     /locus_tag="PLB86_10390"
     CDS             1843..3306
                     /locus_tag="PLB86_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRC65587.1"
                     /translation="MLPLAAALLATACTGSPTPPGDPTASTAPTGSAGVPGVSGMPPT
                     ELSVAGITVVQSMGNDPSLPVAVRRPVAPGQGAMTDAIAAWANATESSFRADNAPSEQ
                     APAELNVGWQVITASGPVLGIRMETFEFGGASGQTTHRVFYGDGSTTWTGMDLVTTAG
                     RAEVAALVRSAVRASGHATLDPDEVGAAGVDALLTDLTFSAAGDLVLLLDQGTVAPFS
                     AGNLTVTVPQATAEPLLSERGRRVADAVRSGLPYAVSVPTSAAPTPVAPATPVAPAPV
                     PPATPAVAPPPAAAVDCTKLKCVALTFDDGPGSLTGTLLDRLEAAGVVATFFVLGQNV
                     RIHPDLVTRMVRSGMVVANHTWDHRDMKRLSLDEARSEVDRTSAEIARVTGVSTTLLR
                     PPYGSMSPQTATLGQAIILWDVDTEDWKNRDASITTSRALATVRPGSIILMHDIHAST
                     IEAVPAIIAGLKARGFTLVTVPQLLGNPQPGKVYTRR"
     gene            complement(3291..3968)
                     /locus_tag="PLB86_10395"
     CDS             complement(3291..3968)
                     /locus_tag="PLB86_10395"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HRC65588.1"
                     /translation="MSTSQSPQPGIPLAVPTEEALAPVPAWGVLSAAAAPVVLAAGYL
                     YAASLTPGFQPVSQPLSDLGAANAGTRWLMAVTLMLVGVSYIVTALGLRPADIAGRWL
                     LGGAGVMMFWVALIPNNVVGKWTLRHILGSAFVFGLLALWPAMSGHLGPRVPWPLRRR
                     VGVMISIVAFVLIELTLWGIILRADTDGAREVLLYAVTALWPLVVVVWTKVRGAGWAY
                     PAVQRRV"
     gene            4012..>5645
                     /locus_tag="PLB86_10400"
     CDS             4012..>5645
                     /locus_tag="PLB86_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HRC65589.1"
                     /translation="MPDTDVIVIGAGLAGLVAATEAVAAGKRVVVVDQESRENLGGQA
                     FWSLGGLFLVDTPEQRRMGIKDSHELAWSDWKGTAGWDRLGSGGEDYWGKQWARAYVE
                     FASGEMRAWLSGLDVKFFPLVGWAERGDGTAGGHGNSVPRFHVTWGTGPGICEPFVRL
                     AKEAEVAGRLRYAFRHRVDDLVFTRGVVSGVRGTRLAPTHVARGVATSREPAGEFELS
                     AGAIIVASGGIGGNLDLVRAAWPPRLGLPPGEAVVGVPAHVDGRMVGITARAGGRIVN
                     GDRMWHYVEGVRNWDPVWAQHGIRIIPGPSSVWLDGAGDRLPAPLYPGFDTLGTLEYL
                     RQSGFDHSWFVLNQRIIEKEFVLSGSEQNLDLTNRSVRDVIGRARGGVPASVQAFLDH
                     GEDFVVAPTVRELVAGMNALVPEAPLDPDKVERILRERDLQVDNEFGKDFQIMAIRAA
                     RAFRGDRMTRTVPPHPILDPAAGPLIAVRLSVLTRKTLGGVQTDLAGRVLRMDGTAVP
                     ALYAAGEVAGFGGGGVHGYRALEGTFLGGCLYSGRVA"
BASE COUNT          809 a         1961 c         2002 g          873 t
ORIGIN      
        1 ccgctcaacg acctgctcgc cgacttcaag cgcaagcgcc agcaaatggc catcgtcatc
       61 gacgaatacg gcggcaccga ggggatcatc accctcgagg acatcctcga ggagatcgtc
      121 ggcgactacg aggacgagtt cacccgggga acgcggcggg tcaagaagct caccggcagt
      181 gcctacgaga tcgacgcctc gatgcggatt tccgacctcg tcgatctggt gagtttcccg
      241 ttccccgagg acgacgacta cgtcacgctc gccggcctgt tctacaaggc cttcggcggg
      301 gtgccctcgg tcggcgacgt cgtcacgctc gaatcggggc ggctcaccgt cctggagatg
      361 gacaaccacc ggatcaccct cgtcaagttc gaggacaccg cccaccaggc cgacgggtcg
      421 gtcggtctcg tcgagagcac ctcggcgccg gacccggtct agggtcacca ccgcgacggt
      481 cggctaggtt tttcggtatg ccgtccgccc ccgtccccgc gtccccgccg gtccgactgt
      541 cggcgcgcgg agcggtcgaa ccgttccatg tcatggaggt catcaaggca gccagcctgc
      601 gccaggccag ccacggcgat gtcatcgcgt tgtgcgccgg ccaaccctcg accccggctc
      661 cggtcgccgc tcgcgtggca gcggtgcgcg cgctggaagg tgaggtgctc ggctataccg
      721 aggcgaccgg catacgcccg ctgcgtgagg cgattgcgcg gcactatggc gactcctacg
      781 ggctggccgt cgaccccccc caggtggtcg tgacgaccgg ttcgtcggcc gccttcaccg
      841 cgttgttcct tgccgccttc gatgtcggcg acacggtggc gatgagccga cccggctacc
      901 cggcataccg aaacacgttg caggcgttgg gtgctcgcgt cgtggagctc gactgtggtc
      961 ccgacacgcg cttccagccg accgtcgcga tgctcgaagc cctgcccgag ccgcccgcgg
     1021 gtctcgtcgt cgcgagcccg agcaacccga cggggacgat catcgacccc gacgagctcg
     1081 ccgcgatcgc ccgttggtgc gaggcgcacg ggacgctgct ggtgagcgac gagatctatc
     1141 acggcatctc gttcgggcgg cggtgcgcca gcgcgtggga gtcctcgcgc tcggccgtgc
     1201 tcgtcgggtc gctgtcgaag tacttctcga tgaccggctg gcgggtcggc tggatggtcc
     1261 tgccgccaga gctggtgcgc ccggtcgagg tgcttatcgg caacctcaac atctgcccgc
     1321 ccgcggtctc gcaggccgcc ggcgtcgccg ccttctccgc gtcggcgcgc gaggagctcg
     1381 acggacacgt gcgccggtat gccgtcaacc gtgacctgct gctgcggcgc ctccccgaga
     1441 tcggcgtggc caaactggcg ccaccggacg gcgcgttcta cctctacttc gacgtcagcc
     1501 acctcaccga cgactcgacg cgctggtgtc aggaggtgct cgacgcaacg ggagtggcct
     1561 tgactcccgg tgtcgatttc gcgccggcga cgccgggagg ggacccggcc ctggatggcc
     1621 gggccttcgt gcggctgtgt ttcgcgggga cgaccgcgga tctggacgac gcgatggatc
     1681 ggctcaccgc ctacctcacg taagcgcttc caccgcctag cgtgggctgc gttgccctat
     1741 tctccctgat cgactgatgt cgatatcgct tgacgaagag ctggacagaa aatgatgcgc
     1801 ggacggttcg cgatgacggt tggtcgacgg tcgaggcgat gggtgctccc cctggccgcc
     1861 gcgctgctgg ccaccgcgtg cacggggtca ccgaccccac ccggcgatcc aaccgcgtca
     1921 acagccccga ccggcagcgc aggcgtcccc ggagtctcag gtatgccgcc caccgagctc
     1981 tccgtggcgg ggatcaccgt ggtgcagagc atgggcaacg acccgtcgct gccggtggcg
     2041 gtgcgccgcc ccgtcgcccc aggacagggg gctatgaccg atgcgatcgc ggcctgggcc
     2101 aacgccaccg agtcgagctt ccgggccgac aacgcgccca gtgagcaggc gccggctgag
     2161 ctcaacgtcg ggtggcaggt catcacggca tccggtcccg tgctcggcat ccggatggag
     2221 acgttcgagt tcggtggtgc cagcgggcag accacccacc gggtcttcta cggtgacggg
     2281 tcgacgacgt ggaccggcat ggacctcgtg accacggcgg ggcgcgctga ggtcgcggct
     2341 ctcgttcggt cggcggtgcg cgcgtcgggt cacgccacgc tcgaccccga cgaggtcggc
     2401 gcagccgggg tcgacgcgtt gctcaccgac ctcaccttct cggccgcggg tgacctggtg
     2461 ctcctgcttg atcagggcac ggtcgcgccg ttctctgcgg ggaacctcac cgtgaccgtc
     2521 ccccaggcga ccgcggagcc gttgttgtcc gagcggggcc gacgggtggc cgacgccgtg
     2581 cgatccggcc tgccgtatgc cgtgtccgtc ccgacctcgg cggcgcccac cccagtcgcg
     2641 ccggctaccc cggtcgctcc tgctccggtg ccgccggcca ctcccgccgt ggcaccgccg
     2701 ccggccgccg ctgtcgactg caccaagctc aaatgcgtcg cgttgacctt cgacgacggc
     2761 ccgggctcac tgaccgggac cctgctcgat cggctcgagg ccgccggagt cgtggccacc
     2821 ttcttcgtcc tgggtcagaa cgtgcggatc catcccgacc tggtgacccg gatggttcgc
     2881 agcggcatgg tcgtggccaa ccacacctgg gaccaccgcg acatgaaacg actctctctc
     2941 gatgaggcgc gctccgaggt cgatcggacc tcggccgaga tcgctcgggt cacgggtgtg
     3001 tccacgacct tgttgcgccc gccctacggc tcgatgtcac cgcagacggc gacacttggg
     3061 caggccatca tcctctggga cgtcgacacc gaggactgga agaacaggga cgcctcgatc
     3121 accacctccc gggctctggc aaccgtgcgc ccggggtcga tcatcctcat gcacgacatt
     3181 cacgcgagca ccatcgaggc cgtgcccgcc atcatcgccg ggctcaaggc gcgtgggttc
     3241 acgttggtga ccgtccccca gttgctgggc aacccccaac cgggcaaggt ctacacccgg
     3301 cgctgaaccg ccggataggc ccagcccgcg ccgcggacct tcgtccacac gacgaccacg
     3361 agcggccaga gggcggtcac ggcatacagg agcacctcgc gcgccccgtc ggtgtccgcg
     3421 cgcaggatga tcccccacaa ggtgagctcg atgagaacga acgcgacgat cgagatcatc
     3481 acgccgaccc gccggcgcag cggccacggc acgcgcgggc cgaggtggcc gctcatcgcc
     3541 ggccacaagg cgagcagccc gaagacgaac gccgacccga ggatgtgccg cagcgtccac
     3601 ttcccgacga cgttgttggg gatcaacgcc acccagaaca tcatcacgcc ggccccgccg
     3661 agcagccagc gcccggcgat gtcggccggc cgcagcccga gcgcggtgac gatgtaggag
     3721 acgccgacga gcatgagcgt gaccgccatg agccagcggg tccccgcgtt ggcggcaccg
     3781 aggtccgaca gcggctggct cacaggctgg aagccggggg tgagcgacgc ggcatacaga
     3841 taacccgcgg cgagcacgac gggagcggcg gccgcgctga gcacgcccca cgcagggacc
     3901 ggcgccagcg cctcctcggt cggcactgca aggggtatgc cgggttgcgg gctttgcgaa
     3961 gtgctcacgt tcctaccgta gacgccgcgc gcgggtgcgt acgatcggcc catgcccgac
     4021 accgacgtca tcgtcatcgg agccggcctc gccggcctgg tcgccgccac tgaagccgtc
     4081 gctgccggca agcgggtggt cgtcgtcgac caggagtctc gggagaacct cggcgggcag
     4141 gcgttctggt ccttgggcgg gctgttcctc gtcgacaccc ccgagcaacg ccggatgggg
     4201 atcaaggact cgcacgagtt ggcgtggtcg gactggaagg gcacggccgg ctgggaccgg
     4261 ctggggtccg gcggcgagga ctactggggc aagcagtggg cccgggccta tgtcgagttc
     4321 gcctcgggcg agatgcgcgc ctggttgtcc gggctggacg tcaagttctt ccccctcgtc
     4381 gggtgggccg agcgcggcga tggcaccgcg ggcgggcacg gcaactcggt gccgcgtttc
     4441 cacgtgacgt ggggcaccgg gccggggatc tgcgagccgt tcgtgcgcct cgccaaggag
     4501 gccgaggtgg ccgggcggct gcgctatgcg ttccggcacc gggtcgacga cctcgtgttc
     4561 acccgaggag tcgtcagcgg cgtccgcggg acccggctag cgccgaccca cgtggcgcgg
     4621 ggtgtcgcga cctcgcgaga gccggcgggc gagttcgagt tgtcggccgg ggcgatcatc
     4681 gtggccagcg gcggcatcgg cggcaacctc gacctcgtgc gggcggcctg gccaccgcgt
     4741 cttggattgc cacccggcga ggcggtcgtc ggggtccccg cccatgtcga cgggcggatg
     4801 gtgggcatca ccgctcgcgc cggtgggcgg atcgtcaacg gcgaccggat gtggcactac
     4861 gtcgaggggg tccgcaactg ggacccggtg tgggcgcagc acggcatacg gatcattcct
     4921 gggccgtcgt ccgtgtggct ggacggggcc ggcgaccggc tgcccgcgcc gctctacccc
     4981 ggtttcgaca cgctcggcac gctggagtat ctgcggcagt cggggttcga tcactcctgg
     5041 ttcgtgctca accagcggat catcgagaag gagttcgtcc tgtcgggctc cgagcagaac
     5101 ctcgacctga ccaatcggtc ggtgcgcgac gtcatcgggc gggcgcgtgg gggcgtgccg
     5161 gcgtcggtgc aggcgttcct cgatcacggc gaggatttcg tcgtggcgcc aaccgtgcgc
     5221 gagctcgttg ccgggatgaa cgccttggtt cctgaggcgc cgctggaccc ggacaaggtc
     5281 gagcggatcc tgcgtgagcg cgatctgcag gtggacaacg agttcggcaa ggacttccag
     5341 atcatggcga tccgggcggc gcgggccttc cggggcgacc ggatgacccg gacggtgccg
     5401 ccgcatccga tcctcgaccc ggcggctggg ccgctcatcg cggtgcggct gtcggtgctg
     5461 acccgcaaga cgctcggggg agtgcagacc gacttggcgg ggcgggtgct gcggatggac
     5521 ggcacggcgg tccctgccct gtatgccgcg ggagaggttg ccgggttcgg cggcggtggg
     5581 gtgcatgggt accgggcgtt ggagggcacc ttcctcggcg ggtgcctgta ttccgggcga
     5641 gttgc
//
LOCUS       DAORPW010000149         5623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_3986_length_5623_cov_751.499282, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000149 DAORPW010000000
VERSION     DAORPW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3986_length_5623_cov_751.499282"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /locus_tag="PLB86_10405"
     CDS             <1..1776
                     /locus_tag="PLB86_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRC65590.1"
                     /translation="RLRQANALDFDDLIMTTVHLLQSFPDVAEHYRRRFRHVLVDEYQ
                     DTNTAQYQLVRELVGVTSDAAGEGGSGASGGSGAGGERAAYAIPPAELVVVGDADQSI
                     YAFRGATIRNIVEFEQDYPNARTILLEQNYRSTQTILRAANSVIARNPDRRPKNLWTD
                     SGDGARIIGYVGDNEHDEAAFIARTIDRLGDEHGVKPKDVAVFYRTNAQSRAIEEVFV
                     RVGLPYKVVGGTRFYERKEVKDALAYLRVISNPTDAVNLRRILNTPRRGIGERAEACV
                     AQLADRERIPFVAALGRAVDAPGIATRSVAAIEAFTRLLEDLRTVFEGGSGVAGLLEA
                     VLEQSGYLRELRNSPDPQDETRVENLAELVAVAQEFDEGRAETGEPASLEDFLEQVSL
                     VADADEIPDPEADAGGVVTLMTLHTAKGLEFPVVFVTGLEDGTFPHLRSLADPKELEE
                     ERRLAYVGITRARERLHLSRAAVRSAWGQPQFFPGSRFLDEIPEELVAWEREAAASDS
                     ARFGRRSSEPAGATLAARPGVRSPGNRAVPVLVAGDRVSHDSFGLGTVLRLEGEGERT
                     LAHVDFGASVGVKRLLLRYAPVEKL"
     gene            complement(1852..2688)
                     /locus_tag="PLB86_10410"
     CDS             complement(1852..2688)
                     /locus_tag="PLB86_10410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRC65591.1"
                     /translation="MTDAKYRGRHRSRRGAGLSPRIVGTGLVLPTTATLAVVSVTGGA
                     PMALTSTTLAAGASTVDDATTDRAELTQDKSTDSTLLARGVQDRVARDSERVRVDTVA
                     QENAKRAATEASLAANDVPVQVSLVGAPGSKLAASAASLKGTNLGSKAWVNPLGGNYS
                     LSSNYGWRWGRLHSGQDMACPTGTPVRSISSGVVTFSGWAGNLGYKVEILHWDGTVSW
                     YGHNSKLRVSEGQRVEPGQLIALAGSTGNSTGPHVHLEIHPNGGNAVPPKRWLAARGV
                     NI"
     gene            2964..3365
                     /locus_tag="PLB86_10415"
     CDS             2964..3365
                     /locus_tag="PLB86_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRC65592.1"
                     /translation="MSDSPLRVVVAKPGLDGHDRGAKVVARALRDAGMEVVYTGLHQT
                     PEQIVEAAIQEDADAVGLSVLSGAHMTLFAKVVELLKVRDAADIVVFGGGIIPDDDLL
                     ALQKLGVAKVFTPGAATHEIVDWVRANVPSH"
     gene            3702..4718
                     /locus_tag="PLB86_10420"
     CDS             3702..4718
                     /locus_tag="PLB86_10420"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HRC65593.1"
                     /translation="MIRPAFAIGLSAAVLVACSSTPSASAPSNPTTTTAGSISTGAAA
                     PTNTLPAVAVGTPLLGPGPVLAVKIDNTSPARPRIGVDAADIVYVEPVEAGLTRLLAI
                     YASTLPPEVGPVRSARESDAVLLGNYGRVAFAFSGASPITTSEIVTGPQVNVSMDSGG
                     AGYRRASDRKAPYNVIGTPAALVARAGGSVPAGDIGFRTGPGAPGGRPATSLATAYPL
                     ARVAFTWDATLGRYLVTTDGRPEVTAAGTPVSAASILVQMIRTHPSNNRDVNGSATPV
                     LELLGTGEAKVLRGGAVYDATWERATNASPTRLTGASGAEVTLAAGPLWVLLVPVGQG
                     VTIS"
     gene            4929..>5623
                     /locus_tag="PLB86_10425"
     CDS             4929..>5623
                     /locus_tag="PLB86_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRC65594.1"
                     /translation="MDLYEYQARDMFEAHGVPVLGGEVAVTPEEAFAAAQRVGAKSGG
                     VVVVKAQVKTGGRGKAGGVKVAKSPEEAQALAAQILGMDIKGHTVKKVMIAQGAKIAE
                     EYYFGLLLDRANRTYLAMCSKEGGMDIETLAVEHPEKLARIAVDAIDGLDEAKAREII
                     AAAGFDEADVPALVNVLVTLGDVYAGEDATLVEVNPLVKTEDGQIVALDGKVTLDDNA
                     RFRHPEHEVLIDHS"
BASE COUNT          882 a         1900 c         1970 g          871 t
ORIGIN      
        1 cggctgcgcc aggccaacgc gctggacttc gacgacctca tcatgacgac ggtgcatctg
       61 ctgcagtcgt tccccgatgt cgctgagcac tatcggcggc ggtttcggca tgtgctggtc
      121 gatgaatatc aggacaccaa cacggcgcag taccaactcg tccgtgagct ggtcggggtc
      181 acgagcgacg ctgcaggaga aggtggttcg ggcgcgagcg gtgggtcggg cgcgggtggt
      241 gagcgggcgg catacgcgat cccgcctgct gagctcgtcg tcgtcggtga cgcagaccag
      301 tcgatctatg ccttccgtgg ggcgacgatc cgcaacatcg tggagttcga gcaggactac
      361 cccaacgcgc ggaccatcct gctggagcag aactaccgct cgacccagac gatcctgcgg
      421 gcggccaact cggtcatcgc gcgcaacccg gaccggcggc ccaagaacct ctggaccgac
      481 tcgggcgacg gggctcggat catcggctac gtcggcgaca acgagcatga cgaggcggcg
      541 ttcatcgcgc ggaccatcga tcggctcggc gacgagcatg gggtgaagcc caaggacgtc
      601 gctgtgttct atcggaccaa tgctcagagc cgggcgatcg aggaagtgtt cgttcgggtc
      661 gggttgcctt acaaggtggt tggcggcacc cggttctacg agcgcaagga ggtcaaggac
      721 gccttggcct atctgcgggt gatctccaac ccgaccgatg cggtcaacct gcggcggatc
      781 ctcaacaccc cgcggcgggg catcggcgag cgggccgagg cgtgtgtcgc ccagttggcc
      841 gaccgcgagc ggatcccctt cgtcgcagcg ctcgggcggg ccgtcgatgc acccggcatc
      901 gccacccgat cggtcgccgc gatcgaggcg ttcacccgac tgttggaaga cctgcggacc
      961 gtcttcgagg gcggctcggg agtggctggt ctgctcgaag cggtgctgga gcagagcggc
     1021 tatctgcgtg agctgcgcaa cagccccgac cctcaagacg agacacgcgt cgagaacctc
     1081 gccgagttgg ttgctgtcgc acaggaattc gacgaggggc gggccgagac cggtgagccg
     1141 gccagcctcg aagacttcct tgagcaggtg tcgctggtcg ctgacgccga cgagatcccc
     1201 gacccggagg ccgatgccgg gggagtggtc acgctgatga ccctgcacac cgccaagggc
     1261 ctggagttcc cggtcgtctt cgtcaccggt cttgaggacg gcaccttccc gcatctgcgc
     1321 tcgctcgcgg accccaagga gctcgaggag gagcggcgcc tggcctatgt cggcatcacc
     1381 cgcgctcgcg agcggctgca tctgtctcgg gctgcggttc gctcggcgtg gggtcagccg
     1441 cagttcttcc ccgggtcacg gttcctcgac gagatccccg aggagttggt ggcgtgggag
     1501 cgcgaagctg cggcgtcgga ttcggcgcgg ttcggccgcc ggtccagcga gccggccggg
     1561 gcgacgctgg cggctcggcc aggggtgcgc tctccgggca accgggcagt gcccgtgctt
     1621 gtcgcgggtg atcgggtgag ccacgactcg ttcgggctcg gaacggtgct gcgcctggag
     1681 ggggaggggg agcgcacgct cgcccacgtc gacttcgggg cgtcagtggg ggtcaagcgg
     1741 ctgctgctgc gttacgcgcc ggtcgagaag ctctgaggcg agtggcgacc ggcgtcagga
     1801 cggagccgac cgtcaggacg ccggaatcgc tcacctgcgg agcacgaagc gtcagatgtt
     1861 gacgccgcga gcagccagcc accgcttcgg cgggacggcg ttgcccccgt tggggtggat
     1921 ctccaggtgc acgtgcggcc cggtcgagtt accggtgctg ccggccaggg cgatgagctg
     1981 acccggctcg actcgctgac cctcgctgac ccgaagcttg ctgttgtggc cgtaccagga
     2041 gacggtgccg tcccagtgga ggatctcgac cttgtagccg aggttccccg cccagccgga
     2101 gaaggtgacg acgccgctgg agatcgaacg caccggcgtg cccgtcgggc aggccatgtc
     2161 ctgaccggag tgcaggcgac cccaacgcca gccgtagttc gagctgaggc tgtagttgcc
     2221 cccgagcggg ttgacccagg ccttggaacc gaggttcgta cccttgaggc tggccgcgga
     2281 agcagcgagc ttggagccag gggcgccgac gagggacacc tggacgggga cgtcgttggc
     2341 ggccagcgac gcttcggttg ccgcccgctt ggcgttctcc tgggcgaccg tgtcgacccg
     2401 cacgcgctcg ctgtcgcggg cgacccggtc ctgcacgccg cgcgccaaca gggtgctgtc
     2461 ggtcgacttg tcctgggtga gctcggcacg gtcggtcgtg gcatcgtcca cggtgctggc
     2521 acccgcggcc agagtggtgc tggtgagggc catcggggct ccgccggtca ctgacaccac
     2581 cgcgagggtg gccgttgtcg ggagcacgag cccggtcccg acgatgcgag ggctgagccc
     2641 ggcacctcgc cgagagcggt ggcgcccacg gtacttggcg tcggtcacgt cgttggtctc
     2701 ttcccgcagc tggaactggc cttgtggagg ttagcgtgat gtattcgtta tctccaagcc
     2761 gggtccacgg gtcgggcgcc catgaccgca gtgtgcgcgg ggccaacgca cctcgtgtga
     2821 tgaggatcac gggaactcgc tgtccaatgg ccgggtgggt cggcggccgg gctggcggct
     2881 gggctggcga cgatggatcc tccgcggtgg cctgggccac tggtcccgct ccacgtgcct
     2941 ggggcttcgc tagagtgcga cgcatgagtg actctccgct gcgcgttgtc gtcgccaagc
     3001 ccgggttgga cggtcatgac cggggggcca aggtcgttgc acgcgccctg cgcgacgccg
     3061 ggatggaagt tgtctacacc ggcctccacc agacgcccga gcagatcgtc gaagcggcca
     3121 ttcaggagga cgccgacgcc gtcggcctgt ccgtcctgtc gggcgcccac atgaccctgt
     3181 tcgccaaggt ggtcgagttg ctcaaggtcc gcgacgcggc cgacatcgtg gtcttcggag
     3241 gcgggatcat ccccgacgac gacctgctgg cgctgcagaa gctcggggtc gccaaggtgt
     3301 tcaccccggg tgcggcgacc cacgagatcg tcgactgggt ccgcgccaac gtcccgagcc
     3361 actaaacctc ggtgtacctc aagccctccg agagtaaaaa gatagtaaga attccctgat
     3421 ctggcaggcg gtggccgggg acagggacca tgatagttcc cagacgtagt cgacacctcc
     3481 cccccaggcg ccggcccgtc gagtcgagaa cgaagtggat gggcccccgg tactcccgca
     3541 tcccgggcga ggccctcgcg ccggcaacgg tgctcactga cttgttcagt gccttcgctt
     3601 cacgacgttg gttgtggggc cacggttacc gtggccccac aacaacgtct agggcagatt
     3661 cgctccagcc cataagttct ccagccccgt agtctgcatt ggtgatccgg cccgcgtttg
     3721 ccatcggcct gagcgccgcc gtgctcgttg cctgctcaag caccccatcg gcctcggccc
     3781 cgtcaaaccc gaccacgacc accgctgggt cgatctcaac gggcgccgcg gcgccgacca
     3841 acaccctccc ggcggtcgcg gtgggcacgc cgttgctcgg cccgggaccg gtgctcgccg
     3901 tcaagatcga caacacgtcg ccggcaaggc cccggatcgg cgtggacgcc gccgacatcg
     3961 tctacgtcga gccggtggag gccggcctca cccggttgct cgccatctat gcctcgaccc
     4021 tcccacccga ggttggtccg gtccggagcg cccgtgagtc cgacgccgtt ctgctgggca
     4081 actacggccg ggtggccttc gccttctcgg gcgcgtcgcc gatcaccacc tcggagatcg
     4141 tcacagggcc gcaggtcaac gtctcgatgg actccggcgg ggctggctac cggcgggcca
     4201 gcgaccgcaa ggccccctac aacgtcatcg gcaccccggc tgcccttgtg gcccgggccg
     4261 gtggcagcgt cccggcagga gacatcggct tccgaaccgg accgggcgcc ccaggcggcc
     4321 gaccggcgac gagtctcgca acggcatacc cactggcgcg ggtcgccttc acctgggacg
     4381 cgaccctcgg ccgctacctg gtgaccaccg acgggcggcc ggaagtgacc gcagccggca
     4441 caccggtcag cgccgcgagc atcctcgtcc agatgatccg aacccacccc tcgaacaatc
     4501 gggacgtcaa cggctccgcc acccccgtgc tcgaactcct cgggacgggc gaggcgaagg
     4561 tgctgcgcgg cggcgcggtc tatgacgcga cctgggagcg ggcaacgaac gcgagtccga
     4621 cccgactcac cggggcgtcc ggcgccgagg tcaccttggc cgccggcccg ctgtgggtgc
     4681 tccttgtccc agtcggccaa ggcgtgacga tctcctgagc ggcggcgcaa ccacggcgca
     4741 acttcgaggc aaccactcgg caaagactcc gcaaccactc cgcggacacc ggccgtgaca
     4801 ttggtcatgg ctggcctccc gacgggcgaa gggcccgaat ccgtgcacta gagtccgaat
     4861 gctgcccgag cggcccaggc ccacggtcgc tgcgctggtc accccttcga cgacgaggac
     4921 ggacgcccgt ggatctgtac gaataccagg cacgtgacat gttcgaagcg cacggggttc
     4981 ccgtgctggg cggcgaagtg gcggtgaccc cggaggaggc cttcgcggcg gcgcagcggg
     5041 tcggcgccaa gagcggcggg gtcgtcgtgg tcaaggccca ggtcaagacc ggtggccgcg
     5101 gcaaggccgg tggcgtcaag gtcgccaagt cacctgagga ggcgcaggcc ctcgccgcgc
     5161 agatcctcgg catggacatc aagggccaca ccgtcaagaa ggtgatgatc gcccaaggcg
     5221 ccaagatcgc cgaggaatac tacttcggcc tgcttctgga ccgggcgaac cgcacctacc
     5281 tggcgatgtg cagcaaggag ggcggcatgg acatcgagac cctcgccgtc gaacaccccg
     5341 agaagctggc ccggatcgcg gtcgacgcga tcgacgggct cgacgaggcc aaggcgcgcg
     5401 agatcatcgc tgcggccggc ttcgacgagg ccgacgtgcc cgcactcgtc aacgtgctgg
     5461 tcaccctcgg cgatgtgtat gccggtgagg acgccacgct cgtcgaggtc aacccgctgg
     5521 tgaagaccga ggacggccag atcgtcgccc tcgacggcaa ggtcaccctc gacgacaacg
     5581 cccggttccg tcaccccgag cacgaggtcc tcatcgacca ctc
//
LOCUS       DAORPW010000150         5590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4017_length_5590_cov_757.392954, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000150 DAORPW010000000
VERSION     DAORPW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4017_length_5590_cov_757.392954"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLB86_10430"
     CDS             complement(<1..698)
                     /locus_tag="PLB86_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HRC65595.1"
                     /translation="MSQVKSDSALAGAVDLARDAAIAIAEPGAVGAHLEVVMEAERLA
                     THYFACDHTAYRGWRWSVSVARAPRQKFATVCETSLVPGADAILAPEWLPYAARIAPG
                     DVGAGDVLPYREDDPLLQAGFEATGDDEVDQLAFAELGLGRKRVLSPEGRVAAAERWY
                     AGSHGPRSEVAEKATDRCVSCGYYLPMAGALRRSFGVCANEWSPSDGAVVSLDHGCGA
                     HSEIDVEAVPAVVVP"
     gene            complement(698..1093)
                     /locus_tag="PLB86_10435"
     CDS             complement(698..1093)
                     /locus_tag="PLB86_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRC65596.1"
                     /translation="MPTGKVKWYDAEKGFGFLSDDDGGDVFLHANALPEGVSTLKGGT
                     RVEFGIVEGRRGAQALSVKVLDPAPSVVASQRAAHRKPSEDMVLIIEDLIKLLDNTSE
                     SLRRGKYPDKNHAGKVAQVLRAVAADLEA"
     gene            complement(1240..1875)
                     /locus_tag="PLB86_10440"
     CDS             complement(1240..1875)
                     /locus_tag="PLB86_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRC65597.1"
                     /translation="MVFTGFPPQALDFYAGLEADNSKAFWTANKGTWESAARDPMLAL
                     LDELEDEFGPATPFRPYRDVRFSADKSPYKTHLGAVAGPTKGVGCYVQLSAEGLLVGG
                     GFHSHGSAQTARYRAAVDAPASGTALEAIVGGLRDKGFAVRGASVKTAPRGFAKDHPR
                     IDLLRMAELMVIREIGTPEWLATPRVVTEVAEAWRAVGPLADWVVEHVGAD"
     gene            1927..4299
                     /locus_tag="PLB86_10445"
     CDS             1927..4299
                     /locus_tag="PLB86_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HRC65598.1"
                     /translation="MGGRPPRAVTGGGRAAYPMVGACDDDLMQRRRGTTPRAPHPAAR
                     SLADDLRHRTDEQLTTLLLARPDLARPTPADLVALAARATTRTSVQRALERLDRGQLQ
                     TVEALVVAAEPNRAGTAAVEDVAALLGGDPTEALDRLWSVGLVWWTSSGPHPVRAVTE
                     VLGPHPGGLGLPAGEVSSEYAAHTPPLPDDLPALLAEAPVAARAILDTLTWGPPIGAV
                     PPSGPAHQGASWLVTHGLCAPSGIGHITLVREVGLALRGGRLHREPALQAPALTDAQV
                     DPAVADRVAGAGARDLLGLLDQLGDLWTALPPRVLRSGALGVRDLAGLARSLDVETAQ
                     AGWLAEVALAAGLIADDGELTPAFSPTPEFDVWRSRSAGARWATVAQAWWESPRAAYL
                     VGTAPNGGATVGALGPEVRWPAVRGVRADVLGELAGLPAGTAPSAESVIERLLWRRPL
                     GDRRALTDAAEAVLREAEWLGARALGTLTSLGRALVDRVPTAELAAAADATLPASVDH
                     VLLQADLTAIAPGPLDGALGRFMRLVSDVESRGGATVLRLSGESVRRALDTGWTADEV
                     LDTLRDSSRTPVPQPLDYLVRDVARRHGQTRVGAATAYIRSDDEAVLAALLADRSLAR
                     AVLRRIAPTVLVSQADPATLVEVLRAGGYSPARESFDGTVVVERPATRRARPPVARPA
                     PEPTLSPVDAALVRSVVVTLRNAPERPVAGAAPPVTLSGSPQETSALLRQAVSDSLAV
                     WIGYADSNGRTARHLVRPVRIDGGRVYAVSGESDSEQLFMLHRITGATTG"
     gene            complement(4469..5401)
                     /locus_tag="PLB86_10450"
     CDS             complement(4469..5401)
                     /locus_tag="PLB86_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRC65599.1"
                     /translation="MRAPQVQADPGLPARVTIYEVGARDGLQNEKSVVPASVKAELIR
                     RLGAAGLPVVETTSFVNPAWVPQLADAAEVLELLGPDGVGSNRPVLVPNIRGLDRALE
                     AGCQAIAIFGSATETFAQRNLGRSIAESVAMFAPVVERARAEGLWVRAYVSMCFGDPW
                     EGDVPVGQVVDAARRLMDLGCDELSLGDTIGVATTGQVPALLDGLDAAGIGRERIAVH
                     FHDTYGQALANTMAALRDGVTIVDSSAGGLGGCPYAKSATGNLATEDLVWALHGAGVE
                     TGVDLEALVATSVWLAGLLGRPSASRVVTALAGS"
BASE COUNT          774 a         2067 c         1947 g          802 t
ORIGIN      
        1 gggaccacga cggcgggaac ggcttcgacg tcgatctccg agtgcgcacc gcagccgtgg
       61 tcgaggctga cgacagcgcc gtccgagggc gaccattcgt tggcacacac tccgaacgag
      121 cggcgcaagg ccccggccat cggcaggtag tagccacagc tcacacagcg atcggtggcc
      181 ttctcagcga cctcggagcg agggccgtgc gaaccggcat accaacgctc ggcggcggcc
      241 acgcgcccct cgggtgagag gacccgcttg cggcccaggc caagctcggc gaaggcgagt
      301 tggtcgacct cgtcgtcgcc cgtggcctcg aagcccgcct gcagcagcgg gtcgtcctcg
      361 cggtagggca gcacgtcgcc cgcgccaacg tcgccggggg cgatccgcgc ggcatacggg
      421 agccactcgg gtgcgaggat cgcgtcggcg ccgggcacga gggacgtctc gcagaccgtc
      481 gcgaacttct ggcgcggtgc gcgggccacg ctcacagacc accgccagcc tcggtatgcc
      541 gtgtggtcgc acgcgaagta gtgcgtcgcc agccgctcgg cctccatgac gacctcgagg
      601 tgagcgccga cggcgccggg ctcggcgatg gctatggctg cgtcacgggc caggtcgacc
      661 gccccggcga gcgccgagtc ggacttgacc tgactcatca cgcctccagg tcggcggcga
      721 cggcgcgcag gacttgggcg accttgccgg cgtggttctt gtcggggtac ttcccccggc
      781 gcaacgactc cgacgtgttg tcgagcagct tgatgaggtc ttcgatgatg aggaccatgt
      841 cttcgctggg cttgcggtgc gcagcccgct gcgaggcgac gaccgacgga gccgggtcga
      901 gcaccttgac cgagagcgcc tgagcgccgc ggcgaccctc aacgatgccg aactcgaccc
      961 gggtgccgcc cttgagggtg ctcacacctt caggaagcgc gttggcatgc agaaagacgt
     1021 cgccgccatc gtcgtcggag aggaagccga aacccttttc cgcgtcgtac cacttgacct
     1081 tgcctgtggg caccgtctca cctcgagctc gtgggccggc gggtccggcc atccgcgcac
     1141 gtcgcgcgag gccaagccta atggggctgg caagtcgatt cgcagcccat gtcgatgagc
     1201 agcccgtcgg cccgtcggcc ggtcagcccg gtcaagcgct cagtcggctc cgacgtgctc
     1261 gaccacccag tccgccaagg gcccgacggc gcgccaggcc tcggcgacct cggtgacgac
     1321 ccggggggtg gccagccact cgggcgtgcc gatctcccgg atgaccatca attcggccat
     1381 ccgcaacaag tcgatccgcg gatggtcctt ggcgaaccct cgcggcgccg tcttcacgct
     1441 ggcgccccgc acggcaaaac ccttgtcccg taggcccccg acgatggcct ccagtgccgt
     1501 tcccgaggcc ggcgcgtcga ccgcagcccg gtagcgagcg gtttgtgccg atccgtgtga
     1561 gtggaagcct ccgccgacga gcaacccctc ggcgctgagc tggacataac acccgacccc
     1621 cttggtcggt ccggccaccg ctccgagatg ggtcttgtag ggggacttgt cggcgctgaa
     1681 ccggacgtcc cgatagggcc gaaagggcgt cgccgggccg aactcgtcct ccaactcgtc
     1741 cagcagggcg agcatcgggt cgcgcgcggc cgactcccag gtgcccttgt tcgccgtcca
     1801 gaacgccttg ctgttgtcag cctccagccc ggcatagaag tccagagcct gtggcgggaa
     1861 ccccgtgaag accatctccg caccctgaca gccgcctgcc gagtaggcaa ctcccgagca
     1921 ggcacggtgg gcgggcggcc tccccgcgcg gtcactgggg gtgggcgggc ggcatacccg
     1981 atggtcgggg cgtgcgacga tgatctgatg cagcggaggc gaggtacgac cccacgcgca
     2041 ccgcatccgg cggcgcgcag cctggccgac gacctccgtc atcgcaccga cgagcaactc
     2101 acgacgctgt tgcttgcccg ccccgacctg gcccgaccga ctccggccga cctcgtcgcg
     2161 ctcgccgctc gggcaacgac ccgcacgagt gtccaacggg ctcttgagcg cctggatcgc
     2221 ggtcagttac agaccgtcga ggccctcgtc gtcgccgccg agccgaaccg cgccggcacc
     2281 gccgcggtcg aggacgtcgc ggccctcctc gggggcgacc ccaccgaggc actcgatcga
     2341 ctctggtcgg tcggcctcgt ctggtggacc tcgtccgggc cgcaccccgt gcgcgccgtg
     2401 acggaagtgc tcgggccgca cccgggtggg ctcggcctcc ccgcgggcga ggtctcctcg
     2461 gagtatgccg cccacacccc tccgctcccc gacgaccttc ccgccctgct cgccgaggcg
     2521 ccggtggcag cccgagcaat cctcgacacg ctcacgtggg gtccccccat cggcgccgtt
     2581 ccgccgtcgg gtccggctca tcagggtgcc tcgtggctgg tcacgcacgg gctgtgtgcg
     2641 ccgagcggga tcgggcacat caccctcgtg cgcgaggtgg ggttggccct gcgtgggggt
     2701 cggctgcatc gcgaacccgc gttgcaggcc cctgcgctga ccgacgctca ggtcgatccg
     2761 gccgtggccg atcgggtggc cggggccggt gcgcgtgacc tgctgggcct gctcgatcaa
     2821 ctcggcgatc tgtggacggc tctgccgccg cgagtgcttc gctcgggggc cctcggcgtc
     2881 cgcgacctcg ccggcctggc tcgctccctc gatgtcgaga ccgcccaggc cggatggctg
     2941 gccgaggtgg cgctcgccgc cgggctgatc gccgacgacg gcgagctcac cccggccttc
     3001 tcgccgaccc ctgagttcga cgtctggcgc tcacgctcgg ctggggcccg gtgggcgacg
     3061 gtcgcccagg cctggtggga gagtccgcgc gcggcatacc tcgtggggac ggcaccgaac
     3121 ggcggggcga ccgtgggcgc actcgggcct gaggtccgct ggccggcggt gcgcggcgtg
     3181 cgcgcggatg tgcttggcga gttggccggg cttccggcgg ggactgcacc gagcgcggag
     3241 tcagtgatcg agcggctgct ctggcggcgg ccgttgggcg accgacgggc gttgaccgac
     3301 gctgccgagg cggtgctgcg cgaggccgaa tggctcgggg ctcgggccct gggcaccctg
     3361 acgagcctcg gtcgggcgct cgtcgatcgg gtgccgacgg ccgagttggc ggcggcggcc
     3421 gacgcgacgc ttccggcgtc agtcgaccac gtgctgctgc aggccgacct caccgcgatc
     3481 gcccccgggc cgctcgacgg cgcgctcggc cgcttcatgc gcttggtctc cgatgtcgaa
     3541 tcccgcggtg gcgcaacggt tctgcggctg tccggcgagt cggtgcgccg agccctcgac
     3601 accgggtgga ccgccgacga ggtcctcgac accttgcgcg actccagccg gacgcccgtc
     3661 ccccaaccgc tggactacct cgtccgcgac gtcgcccggc ggcatgggca gacgcgggtc
     3721 ggcgccgcga cggcatacat ccgctctgat gacgaggcgg tccttgcggc gctgctggcc
     3781 gaccgcagcc tggcccgcgc agtgctgcgc cggatcgccc cgacggtgct cgtgtcgcag
     3841 gccgaccccg ccaccctcgt cgaggtgctg cgggcgggcg gctactcgcc ggcgcgcgag
     3901 tccttcgacg ggacggtggt cgtcgagcgc ccggccacgc ggcgggcgcg accgccagtc
     3961 gcacgccctg ctcctgagcc cacgctgtcg ccggtcgacg cggcgctggt gcgctccgtg
     4021 gtcgttacct tgcgcaacgc acccgagcgg ccggtcgccg gagcagcgcc cccggtgacg
     4081 ttgtccggct cgccgcagga gacgagcgcg ctactgcgcc aagcagtttc cgactcgctt
     4141 gcggtgtgga tcgggtatgc cgacagcaac ggccgcaccg cgcggcacct cgtccgccct
     4201 gtgcgcatcg acggcggccg ggtgtatgcc gtgtccggcg agtccgactc cgagcagttg
     4261 ttcatgctgc accggatcac tggcgccacc accggctgac cgatcactcg gcacctgccg
     4321 cggggcgccc ccatacgccc tgaaaggctc aacttcaggc cgcgggccga ccctcatacc
     4381 ccctgaaaaa agctggtact tcaggtactc actccgtgcc tgaagtacgg ggaaatttca
     4441 cccctcctgc cgcttctcgc ccagggcgtc aggaccccgc gagggcggtg acgacccgcg
     4501 acgcggaggg acgcccgagc agtccggcca gccagacgct cgttgcgacg agggcttcga
     4561 ggtcgacccc ggtctcgaca cccgcaccat gcagggccca gacgaggtcc tcggtcgcga
     4621 ggttgccggt cgccgacttg gcataaggac agccacccag cccaccggcg gacgagtcga
     4681 cgatcgtcac gccgtcgcgc agcgccgcca tggtgttggc caacgcctgc ccgtaagtgt
     4741 cgtggaaatg cacggcgatc cgctcgcgac cgatcccggc cgcatcgagc ccgtcgagca
     4801 gcgccggcac ctggccagtg gtcgccaccc cgatggtgtc ccccaggctg agttcgtcgc
     4861 acccgaggtc catgaggcgg cgagcggcgt cgacgacctg acccaccgga acatcgccct
     4921 cccacgggtc gccgaaacac atcgagacgt aggcccgcac ccacagaccc tccgcccggg
     4981 cgcgctcgac cacgggggcg aacatcgcga ccgactcggc gatgctgcgc ccgaggttgc
     5041 gctgggcgaa ggtctcggtg gccgacccga agatcgcgat cgcttggcaa ccggcctcca
     5101 gggcccggtc cagcccgcga atgttgggca cgagcaccgg gcggttgctc ccgacgccgt
     5161 ccgggccgag caactccagc acctccgcgg catcggccaa ctgcggcacc caggccgggt
     5221 tgacgaacga cgtggtctcg acgacaggca gccccgcggc cccgagacgg cggatgagct
     5281 cggccttgac gctggccggg acgaccgact tctcgttctg cagaccgtcg cgcgccccga
     5341 cctcgtagat cgtcacccga gcgggaagcc ctgggtccgc ctgcacctgt ggagccctca
     5401 tcgccgcctc ctcgcccatg ccacgttgcg ctcgactctc gtccggggat tgtctcaggg
     5461 ctcggcaacc atgagcgatc tcgtgtgagc gggtagtctc gccgcgagga caggagcacg
     5521 cgatgagcga cgtgggcaac acgcccccag gggagtcagc accagacccg gtgggtatgc
     5581 cgcccgcccc
//
LOCUS       DAORPW010000151         5554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4058_length_5554_cov_691.965994, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000151 DAORPW010000000
VERSION     DAORPW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4058_length_5554_cov_691.965994"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLB86_10455"
     CDS             complement(<1..389)
                     /locus_tag="PLB86_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HRC65600.1"
                     /translation="MLRWRRLRRQRLLLSTRHTETPANRERRLTMSAAATFLRDLPRF
                     VFFTGKGGVGKTSLACASAVHLADAGQRVLLVSTDPASNVGQVFGLSIGATITPVPGV
                     PRLDAIEIDPEQAADAYRERIVGPVRDL"
     gene            complement(347..847)
                     /gene="arsD"
                     /locus_tag="PLB86_10460"
     CDS             complement(347..847)
                     /gene="arsD"
                     /locus_tag="PLB86_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HRC65601.1"
                     /translation="MTTAPAPAIRVFEPALCCNTGVCGPDVDQALVTFTADLDHLRGL
                     GVDIERHNLANDPGSFAAEPAVRDFLRVAGSAGLPLTLVDGVTVATGGYPSRERLLSL
                     AGRSEADAVAVAAAVTTQALPTPTTARVDLGLTAAARSTATTSSTATASGGCCGGGDS
                     GGSGCC"
     gene            955..1341
                     /locus_tag="PLB86_10465"
     CDS             955..1341
                     /locus_tag="PLB86_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRC65602.1"
                     /translation="MTATLSSVPGALTTEADVCCPGSACDLLPAAPAAQVAEVFKALA
                     DPTRVRLLHYLAYSASGTACACHLPEALGISQPTLSFHMKKLHDAGLVTREKRGRWVH
                     YTVRAEGLALIREFLDLPTGTDAQCC"
     gene            1332..2063
                     /locus_tag="PLB86_10470"
     CDS             1332..2063
                     /locus_tag="PLB86_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009356599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HRC65603.1"
                     /translation="MLLSSRRAIAEAIGTGGLVATVVGSGIMATRLTQDVALQLLVNA
                     LATVAALAVLIWALGPISGAHFNPVVTLVAVVRREIGAAEAARYVLAQIVGGLAGTAI
                     GNVMFDLPAWQASAKVRSSPSLWLGEVIATAGLLALIGGLTRTGQGRLGPALVPAWIG
                     AAYFFTSSTSFANPAVTIARSVTDTFSGIAPASVPGFVLAQLIGAAIGAALTEFFYPR
                     DGVPEPLDLPAAVHPAPSDAVSHGG"
     gene            2073..2573
                     /locus_tag="PLB86_10475"
     CDS             2073..2573
                     /locus_tag="PLB86_10475"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRC65604.1"
                     /translation="MTRPSPSAQSNEPVSPELFAYLATDPVADLLGIVFEDIRPGYAR
                     ASMPVTHRLLNAPGTAHGGAMMALIDVVHAAVSNSHGTLAVAQDVHTEFLSAGVEGDV
                     LRCEGVEISRTQRTAVFRIDVFADDRPGHPDEKLLATALARVFRIGTPLLPESAASAE
                     SAGAVS"
     gene            2570..3256
                     /locus_tag="PLB86_10480"
     CDS             2570..3256
                     /locus_tag="PLB86_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRC65605.1"
                     /translation="MSSTPEVAEASPDADVLRWPTVVFDLDGTLVDTINLIVSSYQHA
                     FRSVLGHEEDEARIRAWIGQPLLRCFQEASAPHADALFAAYTAWNEANTERLVRPYAG
                     IAELLTDLAGRGARVAVATSKRRPPAQWALALTGLTDLVPVLVTMEDTAVHKPDPTPV
                     LLAVERLGGEPGRAAYIGDAVVDVRAAQAAGMAGIGVLWGAGTDGDLRAAQPHAVAPD
                     VAALRALLLS"
     gene            3390..3677
                     /locus_tag="PLB86_10485"
     CDS             3390..3677
                     /locus_tag="PLB86_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HRC65606.1"
                     /translation="MADTAERVVAAHAPLSDRDREVLAFERQWWKYAGAKEQAIKELF
                     DMSATRYYQVLNALIDNPSALETDPMLIKRLRRLRASRQRQRSARRLGLNP"
     gene            complement(3756..4061)
                     /locus_tag="PLB86_10490"
     CDS             complement(3756..4061)
                     /locus_tag="PLB86_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65607.1"
                     /translation="MNMIPLALEGAWKVLAAGLVLGAGLPIIYAFAVRGLAMAQGAGA
                     IIGEKTAKPFGKVIWWGLLLVILLAVSYGIAFIVATGVGKAVTFDHIIPWFVDKPKK"
     gene            complement(4081..5358)
                     /locus_tag="PLB86_10495"
     CDS             complement(4081..5358)
                     /locus_tag="PLB86_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRC65608.1"
                     /translation="MTTATIILALVVVVALAFDFTNGFHDTGNAMAASIATGALSPKG
                     AVTLAAILNLVGGLLSVEVALTVSNAVVNIQDSKGLPKPELMANGGTMLLLIVFGGLI
                     GGIIWNLATWLLGLPSSSSHALLGGLVGGTIGALGFAGVKWAGDGTKLDGVIGKVLLP
                     SLLSPVIAMIVAATATRVIFMVTARIEDKFREKRFRWGQVGAASMVSLAHGTNDAQKT
                     MGVITLALVAGGSISSTAVYVPFWVKLSAASAIALGTYLGGWRIIHTMGKGLVDISSP
                     QGLAADASSATVILASSQLGFALSTTHVATGSIMGSGIGRKGATVRWSVAGRMVAAWA
                     ITFPAAAVVGAIMYWIAAGLGGLVGPLVVFAILCGFSWYMWKRSRHNVVDHTNVTDEW
                     HDGSKPEVAVAAHDIIDPHHELDEAQPARPTAH"
BASE COUNT          866 a         1845 c         1977 g          866 t
ORIGIN      
        1 aggtcgcgca ccggcccgac gatccgctcg cgataggcgt cggcggcctg ctcggggtcg
       61 atctcgatgg catcgagccg cggcacacca gggaccggcg tgatggtcgc gccgatgctc
      121 agcccgaaga cctgtcccac gttggacgcc gggtcggtgc tgacgaggag cactcgctgc
      181 cccgcgtcgg cgaggtgcac ggcgcttgcg caggccaacg acgtcttacc gaccccgcct
      241 ttgcccgtga agaacacgaa ccggggcagg tcgcgcaaga acgtcgccgc cgcgctcatc
      301 gtcaaccttc gttctcggtt tgcgggtgtc tcggtatgcc gcgtgctcag cagcaaccgc
      361 tgccgccgga gtcgccgcca ccgcaacacc cgccggacgc cgtggccgtt gatgaggtcg
      421 tggccgtcga tcgagccgcg gcggtgaggc cgaggtcgac ccgcgctgtg gtgggagtcg
      481 gcagagcctg ggtggtgaca gcggcggcca cggcgacggc gtcagcctcg gagcggcccg
      541 cgaggctcag caggcgctcg cggctggggt agccgccggt ggcgaccgtc accccgtcga
      601 cgagggtgag cgggagccca gccgacccgg cgacccgcag gaagtcgcgg acggctggct
      661 cggcggcgaa cgacccgggg tcgttggcga gattgtgccg ctcgatgtcg acgccgaggc
      721 cgcgcaggtg gtcgaggtcg gcggtgaagg tgaccagcgc ctggtcgacg tccggcccac
      781 agacgccggt gttgcagcac agcgcgggct cgaagacccg aatggcgggg gctggggcgg
      841 tggtcatggc acgtccttcg tcgaggcaac cggagagcaa cgtatcgacg actctcgata
      901 gaaagtcaac ggcatacatg gacgattgtc aatgtgacgg ctaccatcgg aaacgtgacc
      961 gcgaccctgt ccagcgtgcc tggagccctg acgaccgagg cggacgtctg ctgcccgggg
     1021 tcggcctgtg acctgttgcc ggccgccccg gccgcccagg tggccgaggt gttcaaggcg
     1081 ctcgccgacc ccacccgggt gcgcctgctg cactacttgg cctactccgc gagcggcacg
     1141 gcgtgtgcct gccacctccc cgaggcgctc gggatcagtc agccaaccct gtcgttccac
     1201 atgaagaaac tgcacgacgc ggggctggtc acccgcgaga agcgcggccg ctgggtccac
     1261 tacacggtcc gcgccgaggg actcgcgttg atccgtgagt tcctcgactt gccgaccggc
     1321 accgacgcgc aatgctgctg agctcgcgcc gggccatcgc cgaggccatc ggcaccggag
     1381 gtctggttgc gacggtcgtg ggctcgggga tcatggccac ccggctcacc caggacgtcg
     1441 ccctgcaact tctcgtcaac gcgctcgcga ccgttgctgc cctggccgtc ctcatctggg
     1501 cgctcgggcc gatctcggga gcccacttca acccggtcgt gacgctcgtc gccgtcgtgc
     1561 gccgcgagat cggcgccgcg gaggcggccc gctacgtgct ggctcagatc gtgggtgggt
     1621 tggccgggac cgccatcggc aacgtgatgt tcgacctgcc cgcctggcag gcctccgcga
     1681 aagtgcggtc ctcgccgagc ctttggctgg gcgaggtcat cgcaaccgcg ggcctactcg
     1741 cgctcatcgg cgggctcacc cgcaccggcc agggacggct ggggccggcc ctcgtccctg
     1801 cgtggatcgg cgcggcatac ttcttcacct cttcgacgtc gttcgcgaac ccggcggtga
     1861 ccatcgcccg ttcggtgacc gacacgttct ccgggatcgc gccggcgagc gtcccggggt
     1921 tcgtcctggc tcaactcatc ggagcggcga tcggggcggc cctcaccgag ttcttctacc
     1981 cgcgcgatgg ggttcccgag ccgctcgacc tgccggcagc ggtccacccc gcgcccagcg
     2041 atgcggtgtc tcacggcgga tagggtcggg ccatgacccg cccgtcgcct tccgcgcaat
     2101 ccaacgagcc ggtgagcccc gagttgttcg cctacctcgc caccgacccg gtggccgacc
     2161 tgctcggcat cgtcttcgag gacatccggc cgggttacgc ccgagcgagt atgccggtca
     2221 cccaccgcct gctcaacgcc cccggcaccg cgcacggcgg cgcgatgatg gcgctgatcg
     2281 atgtcgttca tgcggccgtg agcaacagtc acggcacgct cgcggtcgcc caggacgtgc
     2341 acacggagtt cctctcggcc ggggtcgagg gcgacgtgtt gcgctgtgaa ggcgtcgaga
     2401 tcagccgcac gcagcgcacc gccgtcttcc ggattgacgt gttcgccgac gatcggcccg
     2461 gccatcccga cgagaagttg ttggccacgg cgttggcccg ggtgttccgg atcgggacgc
     2521 ccctgctgcc ggagtcggcg gcgtctgccg agtctgcggg ggcggtgtca tgagctcgac
     2581 tccggaggtc gctgaggcgt cgccagacgc ggacgtgttg cgctggccca ccgtcgtctt
     2641 cgacctggac ggcaccctgg tcgacacgat caacctcatc gtctcgtcct accagcacgc
     2701 gttccggtcg gtgttgggcc acgaggagga cgaagcccgg atccgggcct ggatcggcca
     2761 gccgctgttg cgctgcttcc aggaggcctc ggcgccgcac gccgacgcgc tgttcgcggc
     2821 atacaccgcg tggaacgaag ccaacaccga gcggctggtg cggccctatg cggggatcgc
     2881 cgagttgctg accgacctcg caggccgggg ggcgcgggtc gccgtggcga cctccaagcg
     2941 gcgcccgcct gcgcagtggg ccctcgcgct caccgggctg accgaccttg tccccgtcct
     3001 ggtgacgatg gaggacactg cggtccacaa gcccgacccg acgccggtgt tgctcgcggt
     3061 cgagcggctc ggcggggagc cggggcgggc ggcatacatc ggggatgctg tcgtcgacgt
     3121 gcgggcggcg caggcggccg ggatggccgg gatcggggtc ttgtggggcg cggggacgga
     3181 cggggacctg cgggcagcgc agccgcacgc ggtggcgccg gatgtggcgg cgctgcgggc
     3241 gttgttgttg tcctgaccgc gctgcggcag ccccatctgt cacacgtgtc acaggcgcag
     3301 ggcaatcgca aggcgacgcg ccgaggggaa tgacatcgat gtgattcccc tctatagttg
     3361 ccgggggagt cactcagcgg agggttcgaa tggcagacac agcagagcgg gtcgtcgcgg
     3421 cgcatgcgcc gttgagcgac cgcgaccgcg aggtgctggc cttcgagcgg cagtggtgga
     3481 aatacgcggg cgccaaggaa caggccatca aggagctgtt cgacatgtcg gccacgcggt
     3541 actaccaggt cctcaatgcc ctcatcgaca acccgtcggc gcttgagacc gaccccatgc
     3601 tcatcaagcg gctgcgccgg ttgcgcgcga gccgccagcg tcagcgcagt gcgcggcgcc
     3661 tcggcctcaa cccctgactg aaagcacctg cacaaacgcc gaacgggccg gccgcttgat
     3721 tgcgaccgac ccgcccggcg tttctgggct tgggactact tcttcggctt gtccacgaac
     3781 cagggaatga tgtggtcgaa ggtgacggcc ttgccgacgc cggtggcgac gatgaacgca
     3841 atgccgtagg acactgccaa gaggatcacg agcagcaggc cccaccaaat gaccttgccg
     3901 aacggcttgg cggtcttctc gccgatgatc gcaccggcgc cctgggccat cgcgaggcca
     3961 cggacggcga atgcgtagat gatcgggagc cccgcgccga ggacgagccc ggcagcgagc
     4021 accttccacg cgccttcgag ggcgagagga atcatgttca tgatgcgttg ccttcctggc
     4081 tcagtgagcg gtcgggcggg cgggctgcgc ctcgtccaac tcgtggtggg ggtcgatgat
     4141 gtcgtgcgcg gcgacggcga cctcaggctt gctgccgtcg tgccactcgt ccgtcacgtt
     4201 ggtgtggtcg acgacgttgt gccgcgagcg cttccacatg taccaggaga agccgcagag
     4261 gatggcgaag acgacgagcg ggccaacgag gccgccgagt ccggccgcga tccagtacat
     4321 gatcgccccg acgacggctg ctgccgggaa cgtgatcgcc caggcggcga ccatccggcc
     4381 ggcaacggac cagcgcacgg tggcaccctt gcggccgatg ccgctaccca tgatcgagcc
     4441 ggtggccacg tgggtggtcg agagggcgaa gccgagctgg ctcgaggcca ggatgaccgt
     4501 cgccgaggag gcgtcggcgg ccaggccctg gggcgaggag atgtcgacca ggcccttgcc
     4561 catcgtgtgg atgatgcgcc atccgccgag gtaggtgccc agcgcgatgg ccgaggccgc
     4621 ggacaacttg acccagaacg ggacatacac cgcggtcgag ctgatcgatc cgccagcgac
     4681 cagggcgagg gtgatgacac ccatcgtctt ctgggcgtcg ttggtgccgt gggcgaggga
     4741 gaccatcgag gccgcgccga cctgacccca gcggaagcgc ttctcgcgga acttgtcctc
     4801 gatgcgtgcc gtcaccatga agatgacccg cgttgcggtg gccgccacga tcatggcgat
     4861 gactggtgag aggagtgaag gtaggagaac cttgccgatg acgccgtcga gtttggtgcc
     4921 gtcgccggcc cacttcacgc cggcgaagcc gagggcgccg atggttccac cgacgaggcc
     4981 gccgagcagg gcgtgagagg acgaggacgg caggccgaga agccaggtcg cgaggttcca
     5041 gatgatgccg ccgatgagcc cgccgaagac gatgagcaac agcatggtgc cgccgttggc
     5101 catcagctca ggtttgggca aacccttgga gtcttggatg ttgacgacgg cgttgctgac
     5161 ggtgagggca acctccaccg agagcagtcc gccgacgagg ttgaggatcg ctgcgagagt
     5221 cacagcgccc ttcggagaga gcgcccctgt ggcgatggac gccgccatgg cgttcccggt
     5281 gtcatggaag ccgttggtga agtcgaacgc caaggcgacg acgactacca aggcaaggat
     5341 gatggttgcg gtggtcaccc gctcatgatg gggttgacca catggtgtcg ccaagacgaa
     5401 agtcctgaga agttcccgat aacaggcggt aaataggccc tctcaccaag gtttcccgac
     5461 ccgttcatat tccgttcatc cacaaggaac tggcgcccgt gtcctgtgtg ttctgcgtca
     5521 tagccgaggg tggttcggag gccgccgtcg tcct
//
LOCUS       DAORPW010000152         5476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4156_length_5476_cov_702.444567, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000152 DAORPW010000000
VERSION     DAORPW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4156_length_5476_cov_702.444567"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLB86_10500"
     CDS             <1..425
                     /locus_tag="PLB86_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRC65609.1"
                     /translation="FTEVSGTVEVGETLAETSVRAVASAASVSTNQADRDGHLRSADF
                     FDVETYPELTFVSTSVSSDSMIGDLTIKGVTRSVTFDLEFDGVQADPWGNTKAGFTAT
                     TAINRSDWGLTWNAAIEGGGVLVSDKIAITLEVELLKG"
     gene            complement(515..2191)
                     /locus_tag="PLB86_10505"
     CDS             complement(515..2191)
                     /locus_tag="PLB86_10505"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRC65610.1"
                     /translation="MPEPVATRVTLGQAPVACGRVAFVGAGPGDPGLMTVRSRELLGM
                     ADVVVLDDLGEDFVRNFTCADVRVVRVSRHTGSTSGDGATASADGLTDESTDDLGEQL
                     VAEARRLGAGSLVVRLMYGDPMAFHGLAAEALSLRRAGVSFDVVPGVSPLTAAPAYAG
                     VPLTSGCATSVQVVHAGREPMSWGPAVEASVTVVVVGSGPRIAHTLEGLLTAGRDPLT
                     PVAVIEGGTTTLQASRVTTLAKASTLLADNGTGFPVVAVASQTVGLREALSWFETKAL
                     FGWDVLLPRTKEATDSVEERLSAYGARTTVVPTIAVELPRTPQQMERAVKGLVTGHYE
                     WVAFTSANTVRAVRTKVEEFGLDARAFAGVRIAAVGGLTVAALRDWGITADLVPSGEQ
                     SAAGLLEDWPEFDEDVDPMRSVLVPRADIATEALASGLADLGWEVDDVTAFRTVRAAP
                     PAATVREAIKGGAFDAVIFTSSSTVRNLVGIAGKPHPQTIVACVGPATAATAREHGLR
                     VDVVAEVPSSEALIDALAAHGTMLTVQAREAGLPPRRPSEPRPGSRRRSR"
     gene            complement(2293..2964)
                     /locus_tag="PLB86_10510"
     CDS             complement(2293..2964)
                     /locus_tag="PLB86_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRC65611.1"
                     /translation="MVGTLAPRRDIPSATVSRLPVYLRALHEFATRDVTRVSSEELAA
                     QAGVRPTQLRKDLSHLGSHGVRGVGYDVGRLTAELTNALGLNRSWPVAVVGMGNLGRA
                     LAAYSGFATEGFHVEALFDHDPSVIGDLVGRHRIASMGELTDVVARLGIAIGVIATPA
                     NSAQDVADALVGAGVRAILNFAPVALALPEGVACRSVDLATELHVLAFLRQPERVGAD
                     VGRGQ"
     gene            complement(3125..3400)
                     /locus_tag="PLB86_10515"
     CDS             complement(3125..3400)
                     /locus_tag="PLB86_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRC65612.1"
                     /translation="MFGRRKQSVRDAGTPRIVLVGRVGCHLCDEAEAIISRVAAETGA
                     TWVKVSVDDDSALLARWSDQVPVTLVDGRPHDFYRVSEARLRAALKS"
     gene            complement(3417..5042)
                     /locus_tag="PLB86_10520"
     CDS             complement(3417..5042)
                     /locus_tag="PLB86_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRC65613.1"
                     /translation="MPGTSRDAFAAGAARAAGERRKRSSTPLVPADGMPPLPTDEARS
                     APHRPVPQVTPTASANATTVDAVVRERRPRPVLAGLTNVGEATVTQLPTTRGSDRAAG
                     SPRLTPARRTGRRTHREGLPIAEGALTSAVSAPDTSIAPELSSLAADQPAAATSVIQS
                     AATAEAAGESAATTVQASTQASTQASTQASSQSALQASPRVRGDRTGTAYRSRARTPV
                     QAEISESLLASALTQQELTPDIEDIVAAIIGVLRTVAAASGLGADEVEQAVARTLAFL
                     RRRLTGDYSVDDFGLDSDYIENIFLPLMRVLYRQWFRVEVRGIENIPAEGSALIVANH
                     SGTIALDSHMVQVAIHDEHPAARHLRLLGADLVFRTPIMGSLARKQGATLAANSDAER
                     LLSAGEVVGVFPEGFKGVGKPFSERYKLQRFGRGGFVSAAVRTGAPIIPCSIIGAEET
                     YPLLGDVKVLARLFGAPYFPVTPTWPWLGPLGLLPLPSKWIMEFGEPVETARYGAAGA
                     DDPMLVFDLTDQVRETIQQTLYSLLMQRRSVFS"
     gene            complement(5042..>5476)
                     /locus_tag="PLB86_10525"
     CDS             complement(5042..>5476)
                     /locus_tag="PLB86_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HRC65614.1"
                     /translation="VHEDDATAAMLLAVRGPAVQIVNIAGDGIITVTQAAAMVGRPAV
                     PIPFGAAYLGSLVRRAGLADFSTDQLSFLSYGRGLDTTRMREVMKFTPRHTTREAFLD
                     FADHVPPVLPGADTAASLLGRAAGSALQSAGGLAGRIRQVAG"
BASE COUNT          856 a         1917 c         1979 g          724 t
ORIGIN      
        1 gcttcactga ggtcagtggc accgtcgagg tcggcgagac cctcgccgag acctcggtgc
       61 gcgccgtcgc ctctgccgcg tcggtctcca ccaaccaggc cgaccgtgac ggtcacctgc
      121 gcagcgccga cttcttcgac gtcgagacct accccgagct caccttcgtc tccacctccg
      181 tgtcctccga ctcgatgatc ggtgacctga cgatcaaggg cgtcacccgc tcggtcacct
      241 tcgacctcga gttcgacggt gtccaggccg acccgtgggg caacaccaag gccggcttca
      301 ccgcgacgac cgcgatcaac cggtccgact ggggcctgac ctggaacgcg gccatcgagg
      361 gcggcggcgt cctggtgtcc gacaagatcg ccatcacgct cgaggtcgaa ctgctcaagg
      421 gctgacccac cgcggtcgaa cccaccccag ggctcaccac acacgccccg gtatgccgcc
      481 cacgcggcat accggggcgt tcgcgtgtgg gggctcagcg cgagcggcgc cgggacccgg
      541 ggcgcggttc gctgggccga cgaggcggca acccggcctc ccgcgcctgc acggtgagca
      601 tggtgccgtg cgccgcgagc gcgtcgatga gggcctcgga tgacgggacc tcggcaacga
      661 catcgacccg caggccgtgc tcgcgggccg tggcagcggt tgccggaccg acgcaggcca
      721 cgatggtttg cgggtggggc ttgccggcga tgccgacgag gttgcgcacg gtggaactcg
      781 acgtgaagat caccgcgtcg aacgccccac ccttgatcgc ctcgcgcacg gtggcggccg
      841 gcggagccgc ccgcaccgtc cggaaggccg tgacgtcgtc gacctcccag cccagatcgg
      901 ccaggccgga ggcgagcgcc tcggtggcga tgtcggcgcg cggcacgagc accgagcgca
      961 tcgggtcgac gtcctcgtcg aattccggcc agtcctcgag cagcccggcc gccgactgct
     1021 cgccgctggg gacgagatcg gcggtgatgc cccagtcgcg cagcgccgca accgtgagcc
     1081 cgccgacggc ggcgatccgg acgccagcga aggcgcgcgc gtccaggccg aactcttcga
     1141 ccttggtccg caccgcgcga accgtgttgg ccgacgtgaa ggccacccac tcgtagtgcc
     1201 ccgtcaccag gcccttgacc gcgcgctcca tctgctgcgg agtgcgaggc agctcgacgg
     1261 cgatcgttgg aacgacggtg gtgcgcgccc cgtaggccga cagccgctct tcgaccgagt
     1321 cggtcgcttc cttggtgcgg ggcagcagga catcccagcc gaacaacgcc ttggtctcga
     1381 accacgacaa ggcttcgcgc aggccgaccg tctggctggc caccgcaacg acggggaagc
     1441 cggtgccgtt gtctgccaac agggttgacg ccttggccaa ggtggtgacc cggctggcct
     1501 gcagggtcgt cgtcccgcct tcgatgacgg cgaccggggt gagcgggtca cggccggccg
     1561 tcagcaggcc ctcaagggtg tgggcgatcc gcggccccga gccgacgacg acgacggtca
     1621 ctgaggcctc gacggcaggg ccccatgaca tcggctcccg gccggcgtgg acgacctgca
     1681 ccgaggtggc gcagccggag gtgagcggga cgccggcata agcgggagcg gcggtcagcg
     1741 ggctgacccc ggggacgacg tcaaaggaga cacccgcgcg gcgcagcgag agggcctcgg
     1801 cggccaaacc gtggaaagcc atggggtcgc cgtacatcag cctgacgacg agacttccgg
     1861 ccccgagtcg gcgcgcctcg gcgaccaact gctcgccgag atcgtcggtg gactcgtcgg
     1921 tgagcccgtc ggcggacgcg gtcgcgccgt cacctgaggt cgagccggta tgccgcgaca
     1981 cccgcaccac tcgcacatcg gcgcaggtga agttgcggac gaagtcttcg ccgaggtcgt
     2041 cgagcacgac gacatcggcc atcccgagca actcccgcga gcgcaccgtc atgaggccgg
     2101 ggtccccggg cccggcaccg acgaaggcga cccggccgca agcgaccggg gcctgaccca
     2161 gcgtcacccg ggtcgcaacc ggctcgggca cggccagcgc cgttgcggca gaggcggatc
     2221 cagatccgtt tagcgcttgg ccagccagcc tcggctcgtt ggaacgcccc cacccggcga
     2281 gatcgggtcc cttcattggc ccctcccaac gtcggcgccg acccgctcgg gctggcgcag
     2341 aaatgccaag acatgcaatt cggtggccag gtccacggac ctgcaggcca ccccctcagg
     2401 cagcgcgagg gccacgggtg cgaagttgag gatcgcgcgc acccccgccc cgacgagggc
     2461 gtcggccacg tcctgggccg agttggcggg cgtggcgatc acgccgatgg cgatcccgag
     2521 gcgagcgacg acatccgtca actcgcccat ggaggcgatg cggtgacgcc cgaccaggtc
     2581 accgatgacc gacgggtcgt ggtcgaacag cgcctcgacg tggaagccct cggtggcaaa
     2641 cccggagtat gccgcgaggg cgcggccgag gttgcccatc cccacgacgg cgaccggcca
     2701 ggagcggttg aggccaaggg cattggtcaa ctcagccgtg agccgtccga cgtcgtagcc
     2761 aaccccccgg acaccgtgtg agcccaggtg gctgaggtct ttgcgcagtt gggtgggtcg
     2821 cacgcccgct tgagcggcaa gttcctcact ggaaacccgg gtgacgtcac gggtggcgaa
     2881 ctcgtgcaaa gcacgcaggt agaccgggag gcgggacacg gtcgctgaag ggatgtcgcg
     2941 gcgaggggcg agcgtgccga ccacagtcca ccgactcctt cctgcgcagc gttccgggcg
     3001 cagcagggcg cgacgccgcg ggtctggcct ccaatgtagg ggtttgtgaa gaaatgcaca
     3061 aagtgcggaa cgtcacctag aacacccccc ggaatcgctt gcctcccaac gggtttgtcc
     3121 gcgatcagga cttcagggcg gcgcgcaggc gagcctcgga cactcgatag aagtcatggg
     3181 gtcggccgtc gacgagcgtg acgggcacct ggtcgctcca ccgggccagc agggccgagt
     3241 cgtcgtcgac cgacaccttg acccaggtcg ccccggtctc ggccgcgacc cgggagatga
     3301 tcgcctcggc ctcgtcgcac agatggcagc ccacccgtcc gaccaggacg atgcgcgggg
     3361 tgccggcatc ccgcaccgac tgcttgcgcc gcccgaacac cgaggtcgcc gacccgtcag
     3421 gagaagaccg agcgacgctg catgagcaac gagtagagcg tctgctggat ggtctcgcgg
     3481 acctggtcgg tgaggtcgaa gacgagcatc gggtcgtcgg cgccggccgc gccgtagcgg
     3541 gccgtctcga ccggctcacc gaactccatg atccatttcg acggcagcgg caacagcccc
     3601 aacggaccca gccacggcca ggtgggggtc accgggaagt aaggagcgcc gaagagccgg
     3661 gcgagcacct tgacgtcgcc cagcagcgga taggtctcct cagccccgat gatgctgcac
     3721 gggatgatcg gcgcaccggt gcggaccgcc gcgctgacga acccgccacg accgaaccgc
     3781 tggagcttgt agcgctcgct gaacggcttg ccgaccccct tgaaaccctc ggggaacacc
     3841 cccaccactt cgcccgccga caacaggcgc tcggcatcgg agttggcggc cagcgtcgcg
     3901 ccctgtttgc gggccagtga ccccatgatc ggcgtccgga agaccagatc ggccccgagc
     3961 agccgcaggt gccgcgccgc agggtgctcg tcgtggatgg ccacctgcac catgtgcgag
     4021 tccagggcga tggtgcctga gtggttggcg acgatgagcg cggagccctc ggccgggatg
     4081 ttctcgatgc cgcggacttc gacgcggaac cactgccgat agagcacccg catgaggggc
     4141 aggaagatgt tctcgatgta gtccgagtcg aggccgaagt catcgaccga gtagtcgccg
     4201 gtcagccgtc gtcgcaggaa ggccagggtg cgggcgaccg cctgctcgac ctcgtcggcg
     4261 ccgagcccgg aggcagcggc gacggtgcgc aggacgccga tgatcgccgc gacgatgtcc
     4321 tcgatgtcgg gcgtcaactc ctgctgggtg agggccgagg cgagcaggga ctcagagatc
     4381 tccgcctgga ccggggtgcg cgcccgggag cggtatgccg tgccggtccg gtccccccgc
     4441 acgcgcgggg acgcctgcag ggccgattga ctggacgcct gggtggacgc ctgcgtggac
     4501 gcctgagtgg acgcctgaac ggtcgtcgcc gccgactccc ccgccgcctc ggccgtcgca
     4561 gcggactgga tgacactggt cgccgcagcc ggctggtcgg cagccaggct cgacagctcc
     4621 ggcgcgatcg aggtgtccgg cgcgctcacc gccgaggtca gggcgccctc ggcgatcggc
     4681 aggccctcgc ggtgggtgcg tcgaccggtg cgacgcgccg gcgtcagccg ggggctcccg
     4741 gccgcccgat cggaccctcg agtcgtgggg agttgggtga cggtagcctc gccgacgttc
     4801 gtcagcccgg cgaggacggg acggggtcgg cgttcacgaa ccaccgcgtc caccgtcgtc
     4861 gcgttggccg atgcggtcgg agtcacctgg gggacgggcc ggtgtggcgc ggagcgcgcc
     4921 tcgtcggtgg gcaggggcgg cataccgtcg gccgggacca gtggggtgct gctgcgcttg
     4981 cggcgctctc cggcggcccg ggcggcgccc gcggcgaagg cgtcccgcga cgtgccgggc
     5041 atcagccggc cacctgacgg atccggccgg ccagaccgcc ggccgactgc agggcgctac
     5101 cggcggcccg gcccaggagg ctagcggcgg tgtcggcgcc cggaagcacc ggcggaacgt
     5161 ggtcggcgaa gtcgaggaac gcttcgcgag tggtgtgacg gggggtgaac ttcatgacct
     5221 cgcgcatcct cgtcgtgtcc aggccgcgcc cgtaggagag gaaggacagt tggtcggtgg
     5281 agaagtcggc gagcccggcg cggcgcacga gcgacccgag gtatgccgcc ccgaacggga
     5341 tcggcacggc cggccgcccc accatcgcgg cggcctgggt gaccgtgatg atcccgtcgc
     5401 cggcaatgtt gacgatctgg accgccggcc cgcggaccgc cagcaacatg gcggccgtcg
     5461 catcgtcctc gtggac
//
LOCUS       DAORPW010000153         5397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4255_length_5397_cov_701.200861, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000153 DAORPW010000000
VERSION     DAORPW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4255_length_5397_cov_701.200861"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /gene="pheS"
                     /locus_tag="PLB86_10530"
     CDS             <1..710
                     /gene="pheS"
                     /locus_tag="PLB86_10530"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRC65615.1"
                     /translation="TQERLADIFIGMGWEVAEGPELESEWMNFDALNLGKDHPARQMQ
                     DTFFVDPPDGGLVLRTQTSPIQIRSMLTRKPPIYVVAPGKVFRTDDLDATHTPVFHQI
                     ELLAVDKGLTMAHLRGAIDHFVRAMFGDVKTRVRASYFPFTEPSMETDFQCFVCRGAD
                     DACRLCGGTGWIELGGSGMVNRKVFAACGIDPDVYTGFAFGFGIERALQLRNGVSDMR
                     DMVEGDVRFSGQFGMAV"
     gene            712..3270
                     /gene="pheT"
                     /locus_tag="PLB86_10535"
     CDS             712..3270
                     /gene="pheT"
                     /locus_tag="PLB86_10535"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRC65616.1"
                     /translation="MRVPLSWLGELVDLAPGARGAEVAASFVSVGLEEEALHGGDISG
                     PLVVGRVLTFDEQIQKNGKPIRFCTVDVGNHGQRVTDGIPQEIVCGATNFEVGDLVVV
                     VLPGAVLPGGFAISARKTYGRVSNGMICAEDELGLGEDHSGIIVLTDYLGESVAAGLT
                     PGDDALALLGLGEQVLELNITPDRGYCFSMRGIAREYWHSQGSPAGAFRDPGVVPTNP
                     PANLDGYAVHLTDAAPIDGAPGCDRYVARIVRGIDPSASSPAWMRTRLTQAGMRPISL
                     AVDVTNYVMLLVGQPLHAFDLDTLSGSITVRRARVGERLTTLDDVERECSTEDLLITD
                     GGQVPLALAGVMGGATSEVTATTTNILIEAAHFDPVTIARTSRRHRLVSEASKRFERG
                     VDPRVADDAAQLAVDLLVEFGGGTVDPGVTDVDTTSPRTAYPLDVTLPGRLVGVDYSR
                     ARVVQILTDLGCTVEDPGVAPTEVVFVTPPSWRPDLSDGPDYAEEVARVDGYDRIPSV
                     LPTPSDGRGLTHAQVVRRIVANALVGRGFTEVLSYPFVAADFADVLGMTPDDPRRNAV
                     RLANPLSDQAPLMRTSVLDTLLDSLRRNVSRGQRDVALFELGMVTRPLPGSSNTAPVP
                     GVLERPDDETIASIDAAVPPQPRHVAVVLSGDADRAGWWGPGRAADWTDAIDAVRAVA
                     GALSVPVEVTAADLAPWHPGRCAKVALADGTLLGYAGELHPKVLGRLGLPARTCAAEI
                     SVEVLTAASEEPTRLRAFSTQPVALTDVALTVAESVAAGELQAVLREAAGEFLESVIL
                     FDVYRGEQVGADKKSMAFRLAFRAPDRTLTTDEVSRFRDAAVAAAAARFGAVQR"
     gene            complement(3283..4251)
                     /locus_tag="PLB86_10540"
     CDS             complement(3283..4251)
                     /locus_tag="PLB86_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRC65617.1"
                     /translation="MTGPVSLTPPTFDDIQRAADAIHARLPVTPAWSYPALDALAGCE
                     VVVKHENTQPTGAFKVRGGLALAATLTPAHLAAGLVTASTGNHAQSIAYAARLHGATA
                     TIVVPESAPEAKVAAVRLLGANVVVHGPTMTEAVAKAQELAGRDGMTYVDPGNTPAIV
                     AGHATVYLELLTQRPDLEAIYVPIGSGSGAAGACIVRDRLAPDCRIIGVQSSAAPAAY
                     LSWRAGQIETAPCLTRHSGLATGRGFELPQSVIGAGLADFIIVDDAEISDAARVFAHV
                     AHTLAEGAGAAALAGLLKATSHPDRVAVICTGGNASADEIADLARG"
     gene            4337..>5397
                     /locus_tag="PLB86_10545"
     CDS             4337..>5397
                     /locus_tag="PLB86_10545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC65618.1"
                     /translation="MTRRRGIPPSWVVLAAGVATALHLGKLPPAVPVLQAELGMTLVQ
                     AGFMLALIQLAGMFLGLAFGIGADGIGLKRSMVIGLSILSVAGVASGFSHHVGGLLVL
                     RAVEGFGFLLATVPAPGLLRRIVPPDQTTRVLGVWGAYVPFGTALGMLVGPRVLAAFT
                     WPIWWWLVAAGTAVMAAAVWVRVPADPPRSASGSSSVAGPAVPWRERASLTVRSPGPW
                     LGALTFGVYGAQWMAVIGFLPTLYAASGWRGALGATLTATVAAVNVVGNVSAGVLLHH
                     GWRAEVVVGVGFAGQVIGAFLAFATLTESQPVVRFAGALIFSTVGGLVPGGIFALAPT
                     LAPGESTISTTVGWIQQWSS"
BASE COUNT          680 a         1838 c         1974 g          905 t
ORIGIN      
        1 tcacccagga gcggctggcc gacatcttca tcggcatggg ctgggaggtc gccgaaggcc
       61 ccgagctgga gtcggagtgg atgaacttcg acgcgctcaa cctcggcaag gaccacccgg
      121 cccgccagat gcaagacacc ttcttcgtcg acccgcccga cggcgggctg gtgctgcgca
      181 cccagacctc gccgatccag atccgcagca tgctcacccg caagcccccg atctatgtcg
      241 tcgcgccggg caaggtgttc cgcaccgacg acctcgacgc cacgcacacg ccggtcttcc
      301 accagatcga gttgctggcg gtcgacaagg gcctgacgat ggcgcacctg cgtggggcca
      361 tcgaccactt cgtccgggcc atgttcggcg acgtcaagac gcgcgtgcgc gcgtcctact
      421 tcccgttcac cgaaccttcc atggagaccg acttccagtg cttcgtatgc cgtggggccg
      481 acgacgcgtg tcgcctctgc ggagggacgg gctggatcga gctcggcggg tcggggatgg
      541 tcaaccgcaa ggtgttcgcg gcgtgcggga tcgaccccga tgtctatacg ggcttcgcgt
      601 tcggcttcgg catcgagcgc gccctgcaac tgcgcaacgg cgtgagcgac atgcgggaca
      661 tggtcgaggg cgacgtgcga ttctccggtc agttcgggat ggcggtgtga catgagggtt
      721 cccctgtcct ggctcggcga gctcgtcgat ctcgcacccg gtgcccgcgg cgcagaggtc
      781 gccgcgtcgt tcgtctcggt cggtctcgag gaggaggcgc tgcacggcgg cgacatctcg
      841 ggcccgttgg tcgtcgggcg cgtcctgacc ttcgacgagc agatccagaa gaacggcaag
      901 ccgatccggt tctgcacggt cgatgtgggc aaccacgggc agcgcgtcac cgatggcatc
      961 ccgcaggaga tcgtttgcgg tgcaacgaac ttcgaggtcg gggacctcgt ggtggtcgtg
     1021 ctgcccggcg ccgtcctgcc tggcgggttc gccatctcgg cgcgcaagac ctatggccgg
     1081 gtctccaacg ggatgatctg cgccgaggat gagttgggcc tcggcgagga ccactcgggg
     1141 atcatcgtgc tgaccgacta cctcggtgag tcggtggccg caggcctgac gccgggggat
     1201 gacgccttgg cgctcctcgg gctgggtgag caggttctcg agctcaacat cacgcccgac
     1261 cggggctatt gcttctcgat gcgcggcatc gcccgcgaat actggcactc gcagggcagc
     1321 cccgcgggcg ccttccgcga cccgggtgtc gtccccacca acccgccggc caacctcgac
     1381 gggtatgccg tgcacctcac cgacgcggca cccatcgacg gcgcacccgg atgtgaccgc
     1441 tacgtcgccc ggatcgtgcg gggcatcgac ccgtcagcgt cctcaccggc gtggatgcgg
     1501 acccggctca cccaggccgg gatgcggccg atctcgttgg cggtcgacgt caccaactac
     1561 gtcatgctcc tcgtcgggca gcccctgcat gccttcgacc tggacaccct gtcggggtcg
     1621 atcaccgtcc gccgcgcgcg ggtgggggag cggctcacca ccctcgatga cgtcgagcgg
     1681 gagtgctcga cggaagacct gctcatcacc gatggcggtc aggtcccgct ggctctggcc
     1741 ggcgtcatgg gcggggccac cagcgaggtt acggcgacga cgacgaacat cctcatcgag
     1801 gccgcccact tcgacccggt gacgatcgcg cgcacgtcgc gtcggcaccg gctggtcagc
     1861 gaggcctcca agcggttcga gcggggcgtc gatccgcggg tcgctgacga cgccgcccag
     1921 ttggccgtcg acctgctcgt tgagttcggc ggtggcacgg tcgacccggg cgtgaccgat
     1981 gtcgacacga caagtccgcg cacggcatac cccttggatg tcaccctgcc cgggcggttg
     2041 gtgggggtcg actactcccg cgcccgcgtc gtgcagatcc tcaccgactt gggctgcacc
     2101 gtcgaggacc cgggagtggc ccccacggag gtcgtcttcg tcacgccccc gtcgtggcgg
     2161 ccggacctca gcgatggccc ggactacgcc gaggaggttg ctcgcgtcga cggctacgac
     2221 cggatcccgt cggttttgcc gactccgagc gacggccgcg ggctgacgca cgcccaggtg
     2281 gtccggcgga tcgtggccaa cgcgctggtc gggcggggct tcaccgaggt gctcagctat
     2341 ccgttcgtcg cggcggactt cgctgacgtg ctcgggatga ctcccgacga cccccgccgt
     2401 aacgccgtcc gcctggccaa cccgctgtcc gaccaagcgc cactgatgcg gacttcggtc
     2461 ctcgacacgc tgctggattc gttgcgccgc aacgtctctc gtgggcagcg agatgtcgcg
     2521 ctcttcgagc tgggcatggt gacgcggcca ctgccgggtt catcgaacac cgctccggtg
     2581 cctggcgtcc tggagcggcc tgacgacgag accatcgcgt ccatcgatgc ggccgtgccg
     2641 ccgcagcctc ggcatgtcgc ggtcgtcctg tccggtgacg ccgatcgggc agggtggtgg
     2701 ggccccggcc gcgcggccga ctggaccgac gcgatcgacg ccgttcgcgc cgtggccggt
     2761 gcgctctccg ttcctgtcga ggtgaccgcg gccgacctcg cgccctggca ccctggacga
     2821 tgcgcgaagg tcgccctcgc cgacggcacc ctgctcgggt atgccggtga gctgcacccc
     2881 aaggtgctcg gtcgcctggg gctgccggcc cgcacgtgtg cggcggagat ctccgtcgag
     2941 gtgctcaccg cagccagcga agagccgacc cggctgcgcg ccttctcgac ccagccggtg
     3001 gcgttgaccg acgtcgcgct cactgtggcc gagtcggttg ccgcgggcga gttgcaggcg
     3061 gtcttgcgtg aggcggccgg cgagttcctc gagtcggtga tcctcttcga cgtttatcgc
     3121 ggcgagcagg tcggggccga caagaagtcg atggctttcc gcttggcctt ccgcgcccct
     3181 gatcggaccc tgacgaccga cgaggtcagc cggttccgcg acgcggcggt cgctgctgct
     3241 gcggcccggt tcggagccgt ccagcgctga cgacccgctg ggtcagccgc gggcgaggtc
     3301 ggcgatctcg tcggcgctcg cgttgccgcc ggtgcagatc acggccacgc ggtcggggtg
     3361 gctggtggct ttgagcaggc cggcgagggc cgcggcgccg gcgccctcgg cgagagtgtg
     3421 ggccacgtgg gcgaacaccc gggccgcgtc gctgatttcg gcgtcatcga cgatgatgaa
     3481 gtcggccagc ccggccccga tcaccgactg gggcagttcg aatccccgcc ccgtcgccag
     3541 cccgctgtgg cgggtgaggc agggtgccgt ctcgatctgt ccggcccgcc aggagaggta
     3601 tgccgcgggc gccgcactgg actgcacgcc gatgatgcgg cagtccgggg cgagccggtc
     3661 gcggacgatg catgctcccg cggcgccgct gccggacccg atcgggacat agatcgcctc
     3721 gaggtccggg cgctgggtga gcagttcgag atagacggtg gcgtggccgg cgacgatcgc
     3781 cggggtgttg ccgggatcga cgtaggtcat cccgtcgcgg cccgccaact cctgggcctt
     3841 ggcgacggcc tcggtcatcg tcgggccatg gacgacgacg ttcgcgccga gcagccggac
     3901 cgcggccacc ttggcctcgg gtgcactctc gggcacgacg atggtcgcgg tcgctccgtg
     3961 caggcgcgcg gcatacgcga tggattgagc gtggtttccc gtggaagcgg tgacgaggcc
     4021 cgcggcgagg tgcgcggggg tcagggtcgc ggcgagcgcc aggccgccgc ggaccttgaa
     4081 cgccccggtg ggctgggtgt tctcatgctt gacgaccacc tcgcagccgg cgagcgcatc
     4141 gagggccggg tacgaccacg ccggggtgac gggcagtcgg gcgtggattg cgtcggccgc
     4201 tcgctggatg tcgtcgaagg tcggaggagt gagactcacg gggccggtca cccgatgagt
     4261 gtggatcgcc ggtgcccctg cggtccaatc cgccgctgcc cacaacgggt gtcggcggcc
     4321 cggtagattg ccccgagtga cccggcgccg cggcatacct ccctcatggg tggtcctcgc
     4381 ggcgggagtc gcgaccgcgt tgcacctggg caagttgccc ccggcggtgc ccgtccttca
     4441 ggccgagctg gggatgaccc tcgtgcaggc gggcttcatg ctcgccctca ttcagctcgc
     4501 tgggatgttc ctcggattgg ccttcgggat cggtgccgac gggatcgggc tcaagcgcag
     4561 catggtcatc ggcctgagca tcctgtcggt cgcgggggtg gccagcggtt tcagccatca
     4621 cgtcggcgga ctgctcgtcc tgcgcgcggt cgagggcttc gggttcctcc tggcgacggt
     4681 gcctgcgccg gggttgctgc ggcggatcgt cccgccggat cagaccacgc gggtcctggg
     4741 ggtctggggt gcttacgtgc cgttcggaac cgcgctcggc atgctcgtcg ggccgcgagt
     4801 cctggccgcg ttcacgtggc cgatctggtg gtggctcgtg gcggcgggca ccgcggtgat
     4861 ggcagccgct gtctgggtac gcgtgcctgc cgacccgcct cggtccgcgt cggggtcgtc
     4921 gtcggtggcg gggccagcgg tgccctggcg cgagcgggct tcgctcaccg tgcgctcgcc
     4981 gggcccgtgg ctgggcgcgc tcaccttcgg ggtgtacggc gcccagtgga tggccgtcat
     5041 cggcttcctg cctacgttgt atgccgcgtc cggctggagg ggcgcgctgg gcgccacgct
     5101 cacggcgacc gtggccgcgg tcaatgtggt cggcaatgtc agcgctggcg tgttgttgca
     5161 ccatggctgg cgggcggagg ttgtcgtcgg ggtgggcttc gcaggccagg tgatcggcgc
     5221 gtttctggcg ttcgccacgc tgaccgagag ccagccggtg gtgcgattcg ccggggcgct
     5281 gatcttctcg accgtcggcg gcctcgtccc ggggggaatc ttcgcgctgg cgccgacgct
     5341 cgcgccgggc gagtcgacga tctcgacgac ggtcgggtgg atccagcaat ggtcctc
//
LOCUS       DAORPW010000154         5363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4297_length_5363_cov_783.848531, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000154 DAORPW010000000
VERSION     DAORPW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4297_length_5363_cov_783.848531"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLB86_10550"
     CDS             complement(<1..866)
                     /locus_tag="PLB86_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC65619.1"
                     /translation="MLGLDEAELAAYRMLVTGPSGTATEVSGALGVSVGKATRVIASL
                     EAKGLVARSGTAIDHVVASPPDIALGALIVEHQEGIRQAELEMRALRERYLAASSSRA
                     EQVVDVVRGREAVRQRFNQLQRGARREVLEFVKGDVVAVSVDENVEEEAAISRGVTYR
                     FLLERQTLERPGLMAAVSESIRDGVELRVTRELPIRLIVVDREIAMIPLAGATDDQSG
                     GALLLRAGGLLDLAVSLFEVKWRDATRLDAVVDGAVEGSELSARERQILLMLNAGLTD
                     RTVATRLGLSART"
     gene            996..>1531
                     /locus_tag="PLB86_10555"
     CDS             996..>1531
                     /locus_tag="PLB86_10555"
                     /inference="COORDINATES: protein motif:HMM:NF017709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I9 family protein"
                     /protein_id="HRC65620.1"
                     /translation="MKRHQPPGKTLVTAALAISAFGVPLILSASPTQAAGAPPAPAAA
                     SALSQEQRTALTRLTGLQAPAIYVDAKTRASDDSVDVIVELRQGPAASEQARAAAAGR
                     VLSAATAQREAKEAQGRFKQFLTDTFAAKSVTYNIRKTYSDAYNGFALSISGKQLDRL
                     LESADVAAVWEDALVREAT"
     assembly_gap    1532..1631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1632..5057
                     /locus_tag="PLB86_10560"
     CDS             <1632..5057
                     /locus_tag="PLB86_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRC65621.1"
                     /translation="AEGVTGKGLKVAILDTGIDYNHPALKDVYKGGYDFVNDDSDPME
                     TTYADWKASGRAEKDFNGNTYYTRHGSHVAGIIAGKPVAGADAMGIAPEADLYSYRVL
                     GPYGGGTTSNILAGMDRALKDGANVVNMSLGANLNNPHSPLSAAADNLTLAGVTTVLA
                     AGNDGQNGAFSLGSPAASALSITVGANDTPVTVPTLKAAVGSGTADLRLLAQPFGDAL
                     TPLVGTTSDIIDVGRGLSADYSGKVVTGKVVFMRRGDSALENKITLAKTKGAKAVLLA
                     NNLADEGFIPYYLGEGLNFLPTFTLTMAGGTALTDQLAAGATQVTLSDLGTTTFGGGI
                     LATFSSRGPAKLTNDIKPEITAPGVSILSSVPPSAVDTAHLDDYTNAYQRLSGTSMAT
                     PYAAGVAALVLQARPGLKPHEVKVAFMNTADALATEYSVFEMGAGQLDPYQAVHAATE
                     LVVQDRISIADGNSSHDWIPDLTGGLGFGLHATGEVVHDKRDVTVSNKTDQDITYSVR
                     TVYSTSAGVADAAANGVRVSVPSDLRVKAKANMGLTVRLDVPATAAKGSYSGYLLIEG
                     GNAAYRMPFGFRVADKGIAALTALKPVMSTRTENVYSPQLSFAVNLKSHMRNLDAYIV
                     DPASGQDIGYLGALDPIGMREDVNYGPFAWTGKYFPITKDPRAPLGNRLTVLGEGHYQ
                     LRMVGTDDSDQQYSAITDFYVDNTPPKFTTELDGQRVVEVPNGATKYALNGSVIDGEV
                     DKIKAAGIAITQADNLVLRFTFSTIQPSSAHAPQADGTFALTPSFGPSPSATDRYAAI
                     DMAGNVGGRREFLVIKEGAPYIDSKSDKLALAPDQMATHSFTGQNADQWGEVKVTLRY
                     SISNTELGEIRKPAALAAYSTSPLVITKNPLPDYPTVQITIPLDGPQVATGDNLPLLE
                     VETIAKDGNWAAATGFMTVSVTAKKRDGTSVNVNRLFDSVEMLNPTALLRGAFVAQGL
                     LTEDSAIDNTIDYSTIGAAASLTAPDGTVTQVPVSANRALSIDGVAIAADPHLLTVSI
                     PGHFTSYVPVASSFVRPEGLGGRVMAPTVQAAAGDVNNDSVIDILDAIAIRDARGTST
                     RAADINFDGTVDAADLRFVVINFLLRNPEADTVPYPQSTYRGVTLEAVKATFN"
BASE COUNT          918 a         1993 c         1598 g          754 t
ORIGIN      
        1 gtgcgcgccg acagacccaa ccgcgtggcg acggtccggt cggtcagccc ggcgttgagc
       61 atcaacagga tctgccgctc cctcgcggag agctcgctgc cctcgacggc cccgtcgacc
      121 accgcgtcca ggcgcgtggc atcccgccac ttcacctcga acaggctgac ggcaaggtcc
      181 agcagcccgc cggcccgcaa cagcagcgcc ccaccggact ggtcgtcggt ggcgccggcg
      241 agcgggatca tcgcgatctc gcggtcgacg acgatgaggc ggatgggcag ctcgcgggtc
      301 actcggagct ccactccgtc gcggatcgac tccgacacgg ccgccatcag accgggccgc
      361 tccagggtct gacgctcgag gaggaatcgg taggtcaccc cgcgactgat cgccgcctcc
      421 tcctcgacgt tctcatcgac ggacaccgca accacgtcgc ccttgacgaa ctccaacacc
      481 tcccgccggg cgccgcgctg caactggttg aaccgttggc gcaccgcctc gcggcctcgc
      541 acgacgtcga ccacctgctc ggcccggctg ctggacgcgg cgaggtaacg ctcgcgcagt
      601 gccctcatct ccaactccgc ctgccggatg ccctcctggt gctcgacgat gagggcgccg
      661 agcgcgatgt ccggcggcga cgcgacgacg tggtcgatgg ccgttccgga gcgcgcgacg
      721 agccccttgg cctccaacga ggcgatcacc cgggtggctt tgcccacgga cacaccgagg
      781 gcgccggaca cctccgtcgc cgttccggag ggcccggtga cgagcattcg gtatgccgcg
      841 agctcggctt cgtctagtcc caacacactc agcaagcctg caccctcttt cggtgtctga
      901 ccgtggcgag ttcccgccgt gtcgtattca cgatgttgtc acgcctgtgg gccggtgtca
      961 ctatcaagac accccccact cagttcggag cccacatgaa acgacaccag ccccccggta
     1021 agacgcttgt caccgctgca ctggcgatct cggcattcgg cgtccccctg atcctgtcgg
     1081 cctctcccac gcaggcggcc ggcgctcccc cggcccccgc ggcagcatcg gccttgagcc
     1141 aagaacaacg cacggcgctc acccgcctga ccggcctgca ggccccagcc atctatgtcg
     1201 acgccaagac tcgggcctcc gacgactccg tcgacgtcat cgtcgagttg cgccaaggcc
     1261 ccgccgccag cgagcaggct cgggccgccg ccgccggccg ggtgctctcc gcagccaccg
     1321 cccagcgcga ggccaaagag gcccagggcc gcttcaagca gttcctcacc gacacgttcg
     1381 ccgccaagtc ggtgacctac aacatccgca agacctactc cgacgcctac aacggtttcg
     1441 ccctgtcgat ctcgggcaag caactcgatc ggctccttga gtccgccgac gtggcagcgg
     1501 tctgggagga cgccctcgtc cgcgaggcga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn ngccgaaggc gtgaccggca agggcctcaa ggtcgccatc ctcgacaccg
     1681 gcatcgacta caaccacccc gcgctcaagg acgtctacaa ggggggctac gacttcgtca
     1741 acgacgacag cgaccccatg gagacgacgt atgccgactg gaaggcttcc ggccgcgccg
     1801 agaaggactt caacggcaac acctactaca cgcggcacgg ctcgcacgtc gccggcatca
     1861 tcgcgggcaa gccggtcgcc ggcgcagacg cgatggggat cgcccccgag gccgacctct
     1921 actcctaccg cgtgctcggc ccctacggcg gtggcacgac ctcgaacatc ctcgcgggga
     1981 tggaccgggc gctcaaggac ggcgcgaacg tcgtcaacat gtcgctcggc gccaacctca
     2041 acaaccccca cagcccgctc agtgccgcgg ctgacaacct cacgctcgcc ggtgtgacca
     2101 cggtcctcgc tgcgggcaac gacggccaga acggcgcgtt ctccctcggc tcaccggcgg
     2161 catcagcgct gtccatcacc gtgggcgcga acgacacccc cgtcaccgtc cccaccctca
     2221 aggcagcggt cgggtccggc acggccgacc tccgactgct cgcgcagccc ttcggcgacg
     2281 cgctcacccc gctcgtcggg accaccagcg acatcatcga cgtcggccgc ggcctcagcg
     2341 ccgactacag cggcaaggtc gtgacgggca aggtcgtgtt catgcgccgc ggcgacagcg
     2401 cgctcgagaa caagatcacc ctggccaaga ccaagggcgc caaggccgtc ctgctcgcca
     2461 acaacctcgc cgacgagggc ttcatcccct actacctcgg cgagggcctg aacttcctgc
     2521 ccaccttcac cttgacgatg gccggcggga ccgcactgac cgaccagctc gccgccggcg
     2581 ccacccaggt gacgctcagc gacctcggca cgacgacctt cggcggcggc atactcgcca
     2641 ccttcagttc gcgcggcccg gccaagctga ccaacgacat caagccggag atcaccgccc
     2701 ccggcgtgag catcctgtcc tccgtgccgc cgtcggctgt cgacaccgcg cacctggacg
     2761 actacaccaa cgcctaccag cggctctcgg gcacctccat ggccacgccg tatgccgcgg
     2821 gcgtcgccgc cctcgtgctg caggcccggc ccgggctcaa gccccatgag gtcaaggtcg
     2881 ccttcatgaa caccgcggac gcgctcgcca ccgagtacag cgtcttcgag atgggggccg
     2941 gccagctcga cccctaccag gccgtgcacg cagccaccga gctcgtggtc caggaccgga
     3001 tcagcatcgc cgatggcaac agcagccacg actggatccc tgacctcacc ggcgggctcg
     3061 gcttcggcct gcacgccacg ggcgaggtcg ttcacgacaa gcgcgacgtc accgtgtcca
     3121 acaagaccga ccaggacatc acctactcgg tgcgcaccgt ctacagcacg tccgccggag
     3181 tggccgatgc cgctgcgaac ggggtccgcg tgagcgtgcc gtccgacctg cgggtcaagg
     3241 cgaaggcgaa catgggcctc accgtccgtc tcgacgtccc ggccaccgcc gcgaagggct
     3301 cctactcggg ttacctcctc attgaaggcg gcaacgcggc ataccgtatg ccgttcggct
     3361 tccgggtcgc cgacaagggg atcgccgcgc tgacggcgct caagccggtg atgtcgaccc
     3421 gcacggagaa cgtctactcc ccgcagttgt ccttcgcggt caacctcaag tcgcacatgc
     3481 gcaacctcga cgcctacatc gtcgacccgg cgagcggtca ggacatcggc tacctcggag
     3541 ccctcgaccc gatcgggatg cgtgaggacg tcaactacgg gcccttcgcc tggaccggca
     3601 agtacttccc gatcaccaag gacccgcggg cgccgctcgg caaccgcctg accgtgctcg
     3661 gtgaaggtca ctaccagctg cggatggtcg gcaccgacga ctccgaccag cagtactcgg
     3721 cgatcaccga cttctacgtc gacaacaccc cgccgaagtt caccaccgag ctcgacggac
     3781 agcgggtcgt ggaggtgccc aacggcgcca cgaagtacgc cctcaacggc agcgtcatcg
     3841 acggtgaggt ggacaagatc aaggccgccg gcatcgcgat cacccaggcc gacaacctgg
     3901 tgctccggtt caccttctcg acgatccagc cgagctcggc ccacgccccg caagcggacg
     3961 gcacctttgc gctgaccccg agcttcggcc cgagcccgtc cgcgacggac cgctacgccg
     4021 ccattgacat ggccggcaac gtcggcgggc ggcgggagtt cctcgtcatc aaggagggcg
     4081 cgccctacat cgacagcaag agcgacaagt tggcgctggc acctgaccag atggcgaccc
     4141 actccttcac cgggcagaac gccgaccaat ggggtgaggt caaggtgacc ctgcgctaca
     4201 gcatcagcaa caccgagctg ggcgagatcc gcaagcccgc agcgctcgcg gcatacagca
     4261 cctccccgct ggtcatcacg aagaacccgc ttccggacta cccgacggtc cagatcacca
     4321 tcccgctcga cggaccgcag gtggcgacgg gcgacaacct ccccctgctt gaggtggaga
     4381 ccatcgccaa ggacggcaac tgggctgcgg cgaccgggtt catgacggtc agtgtcacgg
     4441 cgaagaagag ggacggcacg tcggtcaacg tcaaccgcct cttcgactcg gtggagatgc
     4501 tcaaccccac cgcgttgttg cggggcgcgt tcgtcgccca ggggctgctc actgaggaca
     4561 gcgccatcga caacacgatc gactactcga cgatcggtgc tgctgcgagc ctgaccgccc
     4621 ccgacgggac ggtgacccag gtgccggttt cggccaaccg ggcgctctcg atcgacgggg
     4681 tggccatcgc cgccgacccg cacctgctga ccgtgtccat ccctggccac ttcaccagct
     4741 atgtgccggt cgcctcgtcc ttcgtccggc ccgagggcct cggaggtcgg gtgatggctc
     4801 ccacggtgca ggctgctgcc ggtgacgtca acaacgacag cgtcattgac atcctcgacg
     4861 ccatcgccat ccgggatgca cgtggcacct cgacccgggc cgcggacatc aacttcgacg
     4921 gcacggtcga cgccgccgac ctgcggttcg tggtgatcaa cttcctcctg cgcaacccgg
     4981 aggcggacac ggtcccctac ccgcagtcga cctaccgcgg cgtcaccctc gaagcggtga
     5041 aggccacctt caactgacag cacacgatga gcccggtggg aacctcttcc caccgggctc
     5101 attcgttggg tggaggtata tctggtcggc acgtccgacc ggatatacct ccaatcagct
     5161 ccatcggggt cggcgggaag gcgacctgca cggcataccg tggagttcat gagcgacgag
     5221 gcactgcgcg acatcctggc gatccccggc ggccgcgggg tcctcaccac tatcaagcgc
     5281 gacgggcgcc cgcagttgtc ggtcgtcttc caccgctggg acgccgacgc gagcaccctg
     5341 tcgatctcgg tgaccgacga tcg
//
LOCUS       DAORPW010000155         5356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4303_length_5356_cov_647.089228, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000155 DAORPW010000000
VERSION     DAORPW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4303_length_5356_cov_647.089228"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..806
                     /locus_tag="PLB86_10565"
     CDS             96..806
                     /locus_tag="PLB86_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65622.1"
                     /translation="MTAAVDIVSLTRRFAAVTACDGMSWSAPRGSITIVLGPNGAGKT
                     TAIECAVGLQRPDDGAVRVLGIDPWLASAEHRAEVGVMLQAGGLPNGSKPLQLLRHLS
                     SFYARPADVGALAARLGIDGFARTTVRRLSGGQKQRVALAAALVGRPQVVFLDEPTAG
                     LDPHGRLDVYDLLKAEQARGATLVVTTHSFEEAATLATHLVVLADGRTVAQGSPAEVV
                     GSATLERVYFDLTRQARR"
     gene            803..1582
                     /locus_tag="PLB86_10570"
     CDS             803..1582
                     /locus_tag="PLB86_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC65623.1"
                     /translation="MTTSTPATPLSTPGPAPARARVLAQARFELATLLANGEQLLVSL
                     VLPLGALVGLAVTTVPSLGDGRRIDLAVPGVLALCVISAAFTAQAIATGFDRRYAVLR
                     LLGTTPLGRDGLLWGKACATLGVEVLQWVVIGAVGVALGWRPVPIGLLYAVPFLLAGT
                     WAFVSLALLLAGALRAEGVLALANLLWVVFLVLGGVIVPRTVLPDALAGVVGILPSAA
                     LADGLRAALSTGAFSIGALVILLAWGAVASAIASRTFRWSD"
     gene            1590..3740
                     /locus_tag="PLB86_10575"
     CDS             1590..3740
                     /locus_tag="PLB86_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRC65624.1"
                     /translation="MNRAPSRTWNSTRVVEALITAGAAAFGLGLGVFLVTGRTMALLE
                     GGIATLVLLLNEAVSRPLLRGLARRGSAVLAVVLGLLSQLVAITLVVALGIRASAGLS
                     FGVGMSLPQAATILAIDAVVISGAQWLVSSNDAEYVLGYVSRRRRRRTTGAGSGLVIV
                     QLDGLGVQVFERALAAGQMPTVARWLHDGTHRLTPWWVPIPSTTPASQAAILFGDDTQ
                     IPSFRFWDRATGRLLVTNRPGDAEVIESGFDGRSGLLAGGGTAISTAFTGGADNSYLV
                     FSRALKPGGLGSGDAFVPLFSSPFLLPRTVMLTVGEVIKELYQSRRQRIRQVQPRIAR
                     RFSFVVLRGLTNVFLRTMNLTIIADKIYEGAPVIFVDFVDYDEIAHHAGPERPESMRS
                     LEGLDGILAALERVIADTPTHYEIVIWSDHGQSLGSTFEQLNGFTLGQRVDQLMDDAT
                     GGSTVVESSTGDEWGPINTLITATVNAMRRGPTSGSGSKELVLGPDRPARTAGRSSDA
                     TPDDGSVPAVIVVGGGNLGTIWFPRLSTRPTLAQIEQHWPALVTGLLATPGVGVVMAS
                     AGGDACVVLAADGVRWIGGDRDGQIEGLDPMRAYDSRGIADLHRLSRLPRCADLVLLS
                     TVDEAGMIHAFEGQVGSHGGLGGPQNQALFISPTALPLDDDLLTQPGDAGERPSFYGA
                     VPIHQQLQRWRTRLAEQQGARQGFSDEQARVWPS"
     gene            3728..4564
                     /locus_tag="PLB86_10580"
     CDS             3728..4564
                     /locus_tag="PLB86_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC65625.1"
                     /translation="MAELSITWLGHSTLRIEVGGARVLTDPVLTHRVGPLSRAVPKPS
                     VRQWADPDVVLLSHLHHDHAHIPSLIRTAPAPILTGAANRAWVIRHRLPGPHPDATPD
                     DWVAITPGVGIRLVRADHESRPMPHRPNDAHGFLIRHDLPTGGHRVVWFAGDTSLYPE
                     MDRLGDLAGADIDVALLPIGGWGPRLSPGHLGPAEAAAAAALSGARHVIGIHYGTLYP
                     RGWPTWHLEWMTRPLVDLAGHLPVRAPAATLHPLSPGATANFSSRSGQLELDVVDESG
                     PA"
     gene            complement(4517..5353)
                     /gene="panC"
                     /locus_tag="PLB86_10585"
     CDS             complement(4517..5353)
                     /gene="panC"
                     /locus_tag="PLB86_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRC65626.1"
                     /translation="MRRHILEWRRAGLRIGFVPTMGNLHAGHYSLVELARAHADRVIS
                     SVFVNPTQFGPSEDFTRYPRTPEADTSGLEAAGCDALWLPTVEGMYPFGIDLASNIRL
                     PGVADVLEGVHRPGHFDGVCTVVARLFNQVQPDVAAFGKKDFQQLAVIAQMVTDLAYP
                     LELLRGEIVRESDGLAMSSRNQYLSPAERSTATVIHRTLRGMRDEMLAGTARLVVEAR
                     AGVELAEAGFEVDYTAVRTPDLVEPSAAPQVYAAPGRRVALIAARLGRTRLIDNIEFE
                     LP"
BASE COUNT          746 a         1976 c         1774 g          860 t
ORIGIN      
        1 cacggcaccc caccgcacca ccgcagccgc ccccgagaca ccagcggggg cggctgcggc
       61 gcgtccgagg cgggccccct cgtagactgc caagcgtgac cgcagcagtt gacatcgtca
      121 gcctcacccg ccgattcgct gcagtcacgg cctgcgacgg catgtcgtgg tcggctccac
      181 gcggctccat caccatcgtc ttgggaccga acggggctgg taagacgacg gcgatcgagt
      241 gcgctgtcgg cctgcaacga ccggacgacg gagccgtgcg ggtcctgggc atcgacccct
      301 ggctcgcctc ggccgagcat cgcgccgagg tgggagtcat gctccaggca ggtgggctgc
      361 ccaacggcag caaacccctc caactgctgc gccacctttc ctcgttctat gcgcggccgg
      421 ccgacgtcgg cgccctggcc gctcggctcg gtatcgacgg gttcgcccgg accaccgtgc
      481 ggcgactgtc gggcgggcag aagcagcggg ttgctctcgc tgcggccctg gtgggtcgac
      541 ctcaggtcgt cttcctcgac gaaccgacgg ccgggctcga cccgcatggg cgcctggacg
      601 tctacgacct gctcaaggcc gagcaggccc gcggcgccac cctcgtcgtc accactcact
      661 cgttcgagga ggccgccacc ttggccacgc atctcgtcgt cctggccgac ggccggactg
      721 tcgcgcaggg ctcgcccgcc gaggtcgtcg gatcagccac cctcgaacgt gtctacttcg
      781 acctcacccg tcaggcccgc cgatgacgac gtccacaccc gccacgcccc tgtcgacccc
      841 cggtccggca ccggcgcgtg ccagggtgct ggcccaggct cgattcgagc tcgccaccct
      901 gctggccaac ggcgagcaac tcctcgtctc actcgtcctg cccctcggcg ccctcgtcgg
      961 gttggcagtc acgaccgtgc ccagcctggg tgacgggcgc cggatcgacc tggctgtccc
     1021 cggagtcctc gccctctgcg tcatctccgc cgcgttcacc gctcaggcga tcgccacggg
     1081 cttcgaccgt cggtatgccg tgctgcgcct gctcggcacc actcccctcg gccgcgacgg
     1141 actgctctgg ggcaaggcct gcgccaccct cggggtcgaa gtcctccaat gggtcgtcat
     1201 cggggcagtg ggcgttgccc tcggttggcg tccggtcccc atcggcctgc tgtatgccgt
     1261 cccgttcctc ctcgccggga cctgggcgtt cgtctcgctg gcgttgctcc tggccggggc
     1321 actccgggcc gaaggcgtcc tcgcgttggc caacctgctc tgggtggtct tcctcgtgct
     1381 tggcggcgtg atcgtgccgc gcaccgtgct gccagacgcg ctcgccgggg tggtcgggat
     1441 ccttccgtcg gcagccctag ccgacggctt gcgagccgcc ctgagcaccg gcgccttctc
     1501 gatcggggcc ctggtcatcc tgctcgcctg gggtgcggtt gcctcggcga tcgccagccg
     1561 caccttccgc tggtcggact gatccgacga tgaatcgagc gcccagccgc acgtggaact
     1621 cgacccgcgt cgtcgaggcg ctgatcacgg cgggggcggc ggccttcggc ctggggctgg
     1681 gggtcttcct cgtcacgggc cgcacgatgg cgctgctcga gggtgggatc gccaccttgg
     1741 tgctgctcct caacgaggcc gtgagccgac cgctgttgcg cggtctggcc cggcgaggca
     1801 gcgctgtcct cgccgtggtt cttggactgc tcagccagtt ggtcgccatc accctggtgg
     1861 tggccctggg gatccgggcc agcgccgggc tctccttcgg cgtcgggatg agcctgcctc
     1921 aggccgcgac gatcctcgcg atcgatgccg tcgtgatctc gggagcccag tggctggtca
     1981 gttccaatga cgccgagtac gtcctggggt atgtcagccg ccgccgacgc cgacggacca
     2041 ccggagccgg cagcggattg gtgatcgtgc agctcgacgg gctgggggtg caggtcttcg
     2101 aacgagccct cgccgcgggg cagatgccga cggtggcacg ctggctccac gacggcacgc
     2161 accgcctgac tccgtggtgg gtccccatcc cttcgaccac gcccgccagc caggccgcga
     2221 tcctcttcgg cgacgacacc cagatcccgt cgttccgctt ctgggaccgc gccaccggca
     2281 ggctcctggt caccaatcgt cccggggacg ccgaggtgat cgaatcagga ttcgacggcc
     2341 gctccgggtt gctcgccgga ggcgggacag cgatcagcac cgccttcacc ggcggtgcag
     2401 acaactccta cctcgtcttc agccgggctc tcaagcctgg cgggctcggc tcgggcgacg
     2461 ccttcgtgcc gctgttctcc agcccattcc tgctgccgcg caccgtcatg ttgaccgtcg
     2521 gagaggtgat caaggagctc tatcagtctc ggcgccagcg gattcggcag gtgcagcccc
     2581 ggatcgcccg ccggttctcg ttcgtcgtcc tgcgcggatt gaccaacgtc ttcctgcgca
     2641 cgatgaacct cacgatcatc gccgacaaga tctacgaggg agcgccggtc atctttgtgg
     2701 acttcgtcga ctacgacgag atcgcccacc atgccggtcc cgaacggccc gaatcgatgc
     2761 gctcgctcga ggggttggac ggcatcctgg ccgccctgga acgcgtgatc gccgacaccc
     2821 ccacccacta cgagatcgtc atctggtccg accacggaca gagcctgggc tcgacgttcg
     2881 aacaactcaa cgggttcacc ctcgggcagc gggtcgatca gttgatggac gatgccaccg
     2941 gtggcagcac cgtcgtggag tcgagcacgg gtgacgaatg ggggccgatc aacaccctca
     3001 tcacggccac ggtgaacgcc atgcgccggg gacccacgtc gggctccggc tccaaggagt
     3061 tggtcctcgg ccccgaccga ccggcccgaa cggccggcag gtcctccgac gccacgccgg
     3121 atgacggctc agtgccagcg gtgatcgtcg tcggcggcgg caacctcggg accatctggt
     3181 tcccccggct gtcgacccga cccacgttgg cgcagatcga gcaacactgg ccggcgctag
     3241 tgaccggcct gctcgccaca ccaggggtcg gggtcgtgat ggcgagtgcc ggcggagacg
     3301 cgtgcgtggt gttggccgcc gacggtgtgc ggtggatcgg cggggaccgc gacggccaga
     3361 tcgagggcct cgacccgatg cgtgcctatg actctcgtgg catcgccgac ctgcaccggc
     3421 tctcgcggct gccacgctgc gccgacctcg tgctgttgtc gacggttgac gaggcgggga
     3481 tgatccacgc cttcgaaggc caggtcggca gccatggcgg cctcggcggt ccgcagaacc
     3541 aggcattgtt catctccccg accgcgctgc cgctggacga cgacctgttg acccaaccgg
     3601 gcgacgccgg ggaacggccg agcttctatg gggcggtgcc gatccaccag cagttgcagc
     3661 gctggcggac caggttggcc gagcagcaag gtgcccggca gggcttttcg gacgagcagg
     3721 cccgcgtgtg gccgagctga gcatcacctg gttgggccat tccaccctgc gcatcgaggt
     3781 gggcggcgcg agggtgctca ccgatccggt cctcacccac cgggtcggtc cgttgtcgcg
     3841 ggccgtgccg aagccgtcgg tccgtcagtg ggccgacccc gatgtcgtgc tcctctcgca
     3901 cctgcatcac gaccacgccc acattccgtc gttgatccgg acggcgcccg ccccgatcct
     3961 cacgggcgcc gcgaaccggg cctgggtgat caggcaccgt ctgcccggtc ctcaccctga
     4021 cgccaccccc gacgactggg tggcgatcac accgggtgtc ggcatccggt tggtgcgcgc
     4081 cgatcacgag tcacggccga tgccccaccg ccccaacgac gcccacggct tcctcatccg
     4141 ccacgacctt cccaccggcg ggcaccgggt cgtgtggttc gccggggaca cctcgctcta
     4201 ccccgagatg gaccgactcg gcgacctcgc cggtgccgac atcgacgtgg ccctgctgcc
     4261 catcggtggc tgggggccgc gcctgtcacc gggccacctc ggccctgccg aggccgctgc
     4321 agcggcagcc ctcagcggcg cccgtcacgt catcggcatc cactacggca ccctctaccc
     4381 gcgcgggtgg ccgacctggc acctcgagtg gatgacccgt cccctcgtcg acctggccgg
     4441 ccaccttccg gtcagggccc cggccgccac cctgcacccg ctgtcgcccg gtgccaccgc
     4501 gaacttctcg tcgcggtcag ggcaactcga actcgatgtt gtcgatgagt cgggtccggc
     4561 ctagccgggc ggcgatcaac gcgacccggc gaccgggggc ggcatacacc tgcggggcgg
     4621 ccgacggctc gaccaggtca ggcgtgcgca ccgcggtgta gtcgacctca aaccccgcct
     4681 cggccaactc cactcccgcg cgggcctcaa cgaccaaccg agccgtgccc gcaagcatct
     4741 cgtcgcgcat cccccgcagt gtgcgatgga tgacggtggc cgtggaccgc tctgcgggcg
     4801 acaggtattg gttgcgcgag ctcatcgcca gaccgtcgga ctcgcggacg atctcgccgc
     4861 gcaacaactc caacggatag gccaggtcgg tcaccatctg cgcgatgacc gcgagttgct
     4921 ggaagtcctt cttgccgaac gcggcgacat cgggctggac ctgattgaac agtcgagcca
     4981 ccaccgtgca caccccgtcg aagtggcccg gccgatgcac tccctcaagc acatcggcga
     5041 ccccaggcag ccggatgttg gaggcgagat cgatcccgaa gggatacatc ccctcgaccg
     5101 tcggcaacca caaggcgtca cacccggccg cttccaagcc gctcgtgtcg gcttccgggg
     5161 tgcgtgggta gcgggtgaag tcctcgctcg ggccgaactg ggtcgggttg acgaacacgc
     5221 tggagatgac ccggtcggcg tgcgcgcggg ccaactccac gagcgagtag tgacccgcgt
     5281 ggaggttgcc catcgtcggg acgaagccga tccgcagccc ggcccgacgc cactcaagga
     5341 tgtggcggcg caacgc
//
LOCUS       DAORPW010000156         5338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4322_length_5338_cov_764.347908, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000156 DAORPW010000000
VERSION     DAORPW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4322_length_5338_cov_764.347908"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLB86_10590"
     CDS             complement(<1..221)
                     /locus_tag="PLB86_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRC65627.1"
                     /translation="MSETIPTVAGDGSIVAGLYRVGDPVEDTSAPAGPIDKKWTTRKF
                     DAHLVNPANRRKLSVIIVGTGLAGGAAAAT"
     gene            complement(235..951)
                     /locus_tag="PLB86_10595"
     CDS             complement(235..951)
                     /locus_tag="PLB86_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRC65628.1"
                     /translation="MATTTLLQGESTARRTSVFMKFLMAVTGALFVLFVLMHMYGNLK
                     ILWGTEAFDEYAEHLRTLLTPILPYSGFLWLFRAVLVLALLVHAYAAFTLWSRANGAR
                     PQRYAVKSAAKGAVRSKMMRWGGVALLAFIAFHLIQFTIVKVNFNGNFSAAQLHGSTG
                     ELVIASFQLWWVTLIYIIAMGALAMHLFHGVWSAAQTLGWTNSAGSRRLAYATAHVVA
                     AVVALGFLVPPLAIFFRLVK"
     gene            1049..2176
                     /locus_tag="PLB86_10600"
     CDS             1049..2176
                     /locus_tag="PLB86_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRC65629.1"
                     /translation="MSGPTQLPGFWAIVPAGGAGTRLWPLSRKGSPKFLHDLTGSGRT
                     LLQQTWDRLTPLAPDRVVVVTGRVHAPEVARQLPGLPAAYLLAEPSPRDSMPAIAWAA
                     AVIAARDPEAVIGSFAADHVISAVEGFHAAVGEAVALARTGRLVTLGITPTYAATGFG
                     YIREGMPLGVDGAPSGRAVVEFVEKPDAERAAAYLASGEFRWNAGMFVVRASVLLELL
                     EQWHPTMVEGVRALAAAPERLDEVWPTLTKIAIDHAVAEPAAAAGRVAVVPATFGWDD
                     IGDFDSLAGLLASDDSDASDDGDASVGGLSIVGESGDVLALDSTGLVVARSGRLVALI
                     GVEDLVVVDTPDALLVVPRARAQEVKGLVDRLADLGREHLR"
     gene            complement(2198..3799)
                     /locus_tag="PLB86_10605"
     CDS             complement(2198..3799)
                     /locus_tag="PLB86_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide antibiotic transporter"
                     /protein_id="HRC65630.1"
                     /translation="MTASLVGTGTLARLAVRRDRVLLPVGILGITIFAVSSAKATLAL
                     YPTVASMDAGIKTIFASPSAVALYGPISDPTDPDALAVVKTTMFGAVLLAIFGYAVVR
                     RHTRSEEEDGRFELVGAGVVGRRAPLTAAVAVATAAVLATCLLTGLGYAAIGMDVAGS
                     LAAAAGWAATGLAFVGLAAVAAQLASTARGCAGLTMGALGVLFLLRAIADAGTGVPGW
                     LGWVSPVGWTSKAEAFGADRSWVVLLGVGLLAVCLVAAFALLQRRDLGAGLLPARRGR
                     ASAGATLAGPVGLAWRLGRSSTIGWTVGMLVGGLVIGSLAKSAMDMFADPAIADLLRK
                     MGGGVGLLSDVYLSTELGFIAIIAAAYGVTTVLRWRAEETQGHTELVLATADSRTRYA
                     ASHLLVALLGTAALVFALGLGTVLADASGGGTLGGAGRVLPAALIRLPAVWVCVGVAV
                     AAIGWFPRWTAAIGWGALAFFLVVGEFGGVFGMPQWLMDLAPFTHVPRMPVEAFAWPP
                     TIALTAVAAGLCIVGVLGFRRRDIG"
     gene            complement(3796..4683)
                     /locus_tag="PLB86_10610"
     CDS             complement(3796..4683)
                     /locus_tag="PLB86_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65631.1"
                     /translation="MSAPIEISGLRKSFGTTVALDGLDLTVEQGEIHGFLGPNGAGKS
                     TTIRVLLGLLRADGGQARLLGGDPWTDVAQLHRRIAYVPGDVALWPNLTGGEVIDLLA
                     RLRGGLNAARRDDLLARFDLDPTKKCRAYSKGNRQKVALVAALSSDAELLLFDEPTAG
                     LDPLMDRVFRDHVDEVRTEGRTVLLSSHILSEVEALCDRVSIIRAGRTVESGSLAQLR
                     HLTRTSVTATLEHPPSAAELAALTGVHDAVLDGSRVTLSADTDHIGAVLSALGAHGIQ
                     TLTSQPPTLEELFMRSYAS"
     gene            4818..>5338
                     /locus_tag="PLB86_10615"
     CDS             4818..>5338
                     /locus_tag="PLB86_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRC65632.1"
                     /translation="MADARSESDLPIKPMYAATDLAAFDAATQLGDAGKYPFTRGVYP
                     SMYTGRPWTMRQYAGFGTAKESNERYHQLVAAGTGGLSVAFDLPTQMGYDSDESISSG
                     EVGKVGVAIDSIDDMRVLFDGLPLDKISTSMTINAPGSTLLLLYQLVAEEQGISGDQL
                     TGTIQNDVLKEYIA"
BASE COUNT          859 a         1800 c         1913 g          766 t
ORIGIN      
        1 gtggcggcag ccgcgccgcc ggccaggccg gtgccgacga tgatcaccga gagcttgcgc
       61 cggttggccg ggttgaccaa gtgggcatcg aacttccggg tggtccactt cttgtcgatc
      121 ggccctgcgg gggcgctggt gtcctcgacc gggtcgccga cccggtagag gcccgcgacg
      181 atcgacccgt cgcccgcgac ggtggggatg gtttcgctca tggtcgtcag ttcctcactt
      241 cacgagccgg aagaagatgg ccaacggcgg caccaggaag ccgagggcga cgacggcggc
      301 gacgacgtgg gccgtcgcgt aggccaggcg ccggctcccg gccgagttgg tccagcccag
      361 ggtctgcgcg gcactccaca ccccgtggaa caggtgcatg gccaacgcgc ccatggcgat
      421 gatgtagatg agcgtgaccc accacaactg gaagctcgcg atgacgagct cgcccgtgga
      481 cccgtggagt tgggccgcgc tgaagttgcc gttgaagttc accttgacga tggtgaactg
      541 gatgaggtgg aaggcgatga acgccagcag cgcgacacct ccccagcgca tcatcttgga
      601 cctgaccgca cccttggcgg cgctcttgac ggcataccgc tgggggcgag ccccgttggc
      661 ccggctccac agcgtgaacg cggcatacgc gtgcacgagc agggcgagga caagcaccgc
      721 gcggaacagc cagaggaatc cggagtacgg caggatcggc gtgaggaggg tgcgcaggtg
      781 ttcggcgtat tcgtcgaagg cctccgtccc ccacaggatc ttgaggttgc cgtacatgtg
      841 catgaggacg aagaggacga agagcgcacc ggtaaccgcc atgaggaact tcatgaagac
      901 ggaggtgcgg cgcgccgtcg actcgccctg aaggagcgtc gtagtggcca cggggtccac
      961 cgtatcgtct gctcacgggc cactggcgcc tcgctacgca cgctgctgtg agatgcctct
     1021 ctcagtcgct ggacgttagc gttgcgccat gagtggtccg acgcagctac caggtttttg
     1081 ggccatcgtc cccgccggtg gtgccggcac gcggttgtgg ccgttgtccc gcaagggttc
     1141 cccgaagttc ctccacgacc tcaccgggtc gggccggacg ttgctgcagc agacgtggga
     1201 ccggttgacc ccgctggcgc ccgaccgggt cgtcgtggtc accggtcgcg ttcacgcacc
     1261 cgaggtggcg cggcaacttc cggggctgcc cgcggcatac ctcctggctg agccgagccc
     1321 ccgtgactcg atgccggcga tcgcctgggc ggctgcggtc atcgcggcgc gcgacccgga
     1381 ggccgtcatc ggatcgttcg cggccgacca cgtgatctcg gccgtcgagg gattccacgc
     1441 ggcagtgggg gaggcggtcg cgctcgcgcg cacgggccgt ctcgtcaccc tggggatcac
     1501 cccgacgtat gccgccaccg gcttcggtta catccgcgag ggtatgccgc tcggtgtcga
     1561 cggcgcgccg tccggtcggg cggtggtgga gttcgtcgag aagcccgacg ccgagcgtgc
     1621 cgcggcctat ctcgcctcag gtgagttccg ttggaacgcg gggatgttcg tcgtcagggc
     1681 gagcgtgctg ctggagttgc tggagcagtg gcatccgacg atggtcgagg gcgtgcgggc
     1741 gctggccgcc gccccggagc gactggacga ggtctggccg accctgacga agatcgccat
     1801 cgaccacgcg gtcgccgagc ccgctgctgc ggcggggcgg gtggcggtgg tgccggcgac
     1861 gttcggatgg gatgacatcg gcgatttcga ctcgctggcc gggctgctcg ccagtgacga
     1921 cagtgatgcc agtgacgacg gtgacgcgag tgtcggcggg ctctcgatcg tcggggagtc
     1981 gggggacgtc ctcgcgttgg actcgaccgg gttggtcgtc gcccgctcgg gtcggctggt
     2041 ggccctgatc ggtgtcgagg acctcgtcgt cgtcgacacc ccggacgcgc tcctcgtcgt
     2101 gccgagggct cgggctcagg aggtcaaggg gctcgtcgac cgacttgccg acctcggccg
     2161 cgaacacctg cgctgagcca cgggtgccct gagcccctca gccgatgtcg cggcggcgga
     2221 agccgaggac gccgacgatg cacaacccgg cagcgaccgc tgtcagcgcg atcgtggggg
     2281 gccacgcgaa cgcctcgacg ggcatccgcg gaacgtgggt gaacggcgcg agatccatca
     2341 gccactgcgg catcccgaac acgccaccga actcgccgac gacgaggaag aaggccagcg
     2401 ccccccaccc gatcgccgct gtccatcggg gaaaccaccc gatggctgcg accgcgacgc
     2461 ccacgcacac ccagaccgca gggagccgga tcagcgcagc gggcaggacc cgaccggctc
     2521 caccgagggt cccgccgccg gacgcatcgg ccaggacggt gccgaggccg agcgcgaaga
     2581 ccagggcggc ggtgccgagc agggcgacaa gcaggtggct cgcggcatac cgagtgcgcg
     2641 agtcggccgt ggcgaggacg agctcggtgt gcccctgcgt ctcctccgcg cgccagcgca
     2701 gcacggtcgt gacgccgtat gccgcggcga tgatcgcgat gaagcccaac tccgtggaca
     2761 gatagacatc ggacaacagg ccgacgccgc cacccatctt gcgcaacagg tcggcgatcg
     2821 ccgggtcggc gaacatgtcc attgcactct tggcgagcga gccgatgacc aggccgccga
     2881 cgagcatccc caccgtccac ccgatcgtcg aactgcgacc gagccgccag gccaacccga
     2941 ccggcccggc gagggtggcg ccggcgctgg ctcgaccccg ccgcgcaggc agcagacccg
     3001 cgccgaggtc gcgtcgttgc agcaacgcga aggcggccac gaggcagacg gccagcagcc
     3061 ccacgccgag gagcacgacc caggaacggt ccgccccgaa ggcttcggcc ttgctcgtcc
     3121 acccgaccgg cgagacccag cccaaccagc ccgggacccc cgtgccggcg tcggcgatgg
     3181 cgcgcagcag gaacaacacg ccgagggcgc ccatcgtcag accggcgcag ccgcgagcgg
     3241 tggaggcgag ttgggcggcg acggctgcga ggccgacgaa cgccaatccc gttgccgccc
     3301 agccagctgc ggccgcgagt gagccggcca cgtccatgcc gatggcggca tacccgaggc
     3361 cggtgagcag gcacgtggcc aggactgcgg cggtcgccac ggccacggcg gcagtgagcg
     3421 gagcccggcg cccgaccacg cccgccccga cgagctcgaa gcggccgtcc tcctcctcgg
     3481 agcgggtgtg gcgacgcacg acggcatacc cgaagatcgc gaggagcact gccccgaaca
     3541 tcgtcgtctt gacgacggcg agggcgtcgg ggtcggtcgg gtcggagatt gggccataga
     3601 gcgccacggc cgacgggctg gcgaagatcg tcttgatgcc ggcgtccatc gacgcgacgg
     3661 tcggatagag cgcgagggtc gccttggcac tgctcaccgc gaagatcgtg atcccgagga
     3721 tgccgacggg cagcagcacc cggtcgcgtc gcacggccag gcgggcgagc gtccccgtgc
     3781 cgaccaggct cgcggtcatg acgcgtagct cctcatgaac agttcctcga gcgtgggcgg
     3841 ctggctggtc agggtctgta tgccgtgtgc cccgagcgcg ctcagcaccg cgccgatgtg
     3901 gtcggtatcg gccgagaggg tcaccctcga cccgtcgagc acggcatcgt gcacgcctgt
     3961 cagcgcggcc aattcggctg cgctgggcgg gtgttcgaga gttgccgtca ctgaggtccg
     4021 ggtgagatgg cgcaactgag cgaggctgcc ggactcgacg gtgcgtccgg cccggatgat
     4081 gctcacccgg tcacagagcg cctcgacctc ggagaggatg tggctggaga gcaggaccgt
     4141 gcgcccctcg gtgcgcacct cgtcgacgtg gtcgcggaag acgcggtcca tcagcgggtc
     4201 gagccccgct gtcggctcgt cgaacaacag caactcggcg tctgaggaca gtgctgcgac
     4261 aagggcgacc ttctgccggt tgcccttgga gtaggcacga cacttcttgg tcgggtcgag
     4321 gtcgaagcgc gcgagcagat cgtcccgcct ggccgcgttg aggcccccgc gcagtcgggc
     4381 gagcaggtcg atgacctcgc cgccggtgag gttgggccag agggcgacat cgccggggac
     4441 gtaagcgatc cggcggtgca actgcgccac gtcggtccac gggtcgccgc ccagcaatcg
     4501 ggcctggcca ccgtcggcgc gcaacaaccc gagcaggacc cggatcgtcg tcgacttgcc
     4561 cgcgccgttg ggtccgagga aaccgtggat ctcgccctgc tcgacggtga ggtccaggcc
     4621 atcaagggcg acggtggtgc cgaaggactt tcgcagcccg ctgatctcga tcggagcgct
     4681 catagttcca tggttacacc acgaccgccg gcctgcctcg actgccctcg gtgttcgccg
     4741 cggctgtcgg cacacggcat acgagagtga catcagtccc atgaccgcgg tggtgagccg
     4801 ccactaggct gaaacgcatg gctgacgcgc gctccgagtc cgaccttccg atcaagccga
     4861 tgtatgccgc gactgacctc gcggcattcg acgcagcgac ccagttgggt gacgcaggca
     4921 agtacccgtt cacccgtggc gtctacccgt cgatgtatac gggccggccg tggacgatgc
     4981 gtcagtacgc cgggttcggg acggccaagg agtccaacga gcgctaccac caactcgtcg
     5041 cggcgggcac tggcgggctg tcggtggcct tcgacctgcc gacccagatg ggctatgact
     5101 ccgacgagtc gatctcctcc ggcgaggtcg gcaaggtcgg cgtggccatc gactcgatcg
     5161 acgacatgcg ggtgctgttc gacgggctgc cgctggacaa gatctcgacg tcgatgacga
     5221 tcaacgcgcc cggctcgacg ctgctgctgc tctaccagct ggtcgcagag gagcagggca
     5281 tctccggcga ccaactcacc gggacgatcc agaacgacgt gctcaaggag tacatcgc
//
LOCUS       DAORPW010000157         5280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4398_length_5280_cov_873.030431, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000157 DAORPW010000000
VERSION     DAORPW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4398_length_5280_cov_873.030431"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLB86_10620"
     CDS             <1..428
                     /locus_tag="PLB86_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HRC65633.1"
                     /translation="AMGGNMLDLALGFIIGAAFATLVESLASNILMQLVAAIFGTPDF
                     TELSLTLNNAEITYGAFLTDFVGFILMALVLFAIVKLLKKAGMGNFRAQGQRECPMCK
                     EFVAVDALKCKWCTADIDPDQAEEEDLEIIEARRKPEQP"
     gene            425..754
                     /locus_tag="PLB86_10625"
     CDS             425..754
                     /locus_tag="PLB86_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HRC65634.1"
                     /translation="MSADYAFRAPLWRWQGESPWHFVTLPEEQSDEIDERTRDVQRGF
                     GSIRVEVTVGTTVWATSLFPSTNHGAYLLPVKKAVRVAEGLTADEPVDVRLRLLDLPG
                     GAPGSRG"
     gene            1100..4402
                     /gene="ileS"
                     /locus_tag="PLB86_10630"
     CDS             1100..4402
                     /gene="ileS"
                     /locus_tag="PLB86_10630"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRC65635.1"
                     /translation="MVYPKASTDPEATGVPPTPRFPTIEERVLAYWAKDGTFLASVEA
                     RDGGTDGSNEFVFYDGPPFANGLPHYGHLLTGYVKDLVPRYRTMRGRRVERRFGWDTH
                     GLPAELEAQRQLGLKTTQEITDLGIATFNDACRNSVLRYTKEWEEYVTRQARWVDFEH
                     DYKTLDTDYMESVIWAFKQLHEKGLAYEGFRILPYCWNDQTPLSNHELRMDDDVYQMR
                     QDPAITVGYRLETGELALIWTTTPWTLPSNMGVAVHPDVDYVVVQGDKDGGSDRYLLA
                     QARLAAYTRELGDDAAERIVARYKGSELVGRRYTPPFTYYLGYAGAHQISAAGFVTTE
                     DGTGLVHMSPGFGEDDKLALDALGVETVVPVGPDGCFVFPVTDYAGLHVFDANHPVID
                     HLKARTRGHGEMGSTTEGTVLVRHETYDHSYPHCWRCREPLIYMAVSSWFVATTAIRD
                     RMLELNQEITWVPEHTKDGQFGKWLENTRDWSISRNRFWGSPIPVWKSDDPTYPRVDV
                     YGSFAELERDFGVPVTDLHRPFIDSLTRPNPDDPRPEGERSTMRRVTDVLDCWFESGS
                     MSFAQVHYPFENADWFEHHFPGDFIVEYIGQTRGWFYTMHVLATALFDRPAFKTCISH
                     GIVLGSDGQKMSKSLRNYPDVFEVFDRDGADAMRWFLMSSPILRGGNLVVTEGGIRDG
                     VRQVMIPLWNAWHFFALYANAAGGGKGYAATASTASTDLLDRYLLAKLREFVEAMTAQ
                     LDSYDIAAACDTMRSFLDVLTNWYIRRSRDRFWDSDNSLDASTTAAFDTLYTALEVLT
                     RAVAPLLPLTTEEIWRGLTGGRSVHLEDWPDAAALPADHALVAGMDRAREVCSAVSSL
                     RKGASLRARLPLAELVVVTTDPAALEPFSAIIADEVNVRSVVLRDVELAHESDFGVMR
                     RLSVNARVAGPRLGKEVQTAIRAAKAGDWSTAPDGTVTAGGIALVDGEYAVETVVAGQ
                     DARTAGSTDQGSGPSHAVAVLPAGGFVVLDTEVTPELAVEGVANDLVRAVQQTRRESG
                     LHVSDRISLSLAGAELMQRAAHEHRDRLMAQTLAEHLSVATHVEDLALGDGVVETSVG
                     DALPVRIKVAKR"
     gene            4402..4899
                     /locus_tag="PLB86_10635"
     CDS             4402..4899
                     /locus_tag="PLB86_10635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC65636.1"
                     /translation="MGVVIRPATAYDVDAVVDVGRRTWPQTYTPLAGEDYVRMGLDKW
                     WTVAATRPLVLAGKATVAELDGEVVGVAVVGPLTGDLVLFRLYVVPEHQGMGIGRLLL
                     EDVMTTARETGHRILRLSYLDGNAGAERFYQGFGFVESHREPTGDGIPDSIWVVRDLW
                     EQEQS"
     gene            4896..>5280
                     /locus_tag="PLB86_10640"
     CDS             4896..>5280
                     /locus_tag="PLB86_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRC65637.1"
                     /translation="MTPPPSDPAVAARLAQVEAEIMGRAPEHDLVPTLDRVAAVMELL
                     GDPQRSFRVIHLTGTNGKTSTTRMIEALCREHGLRTGRFTSPHLHSMRERIAVDGEPV
                     SAEVLVETWDEVAPIIEIVDARSAAE"
BASE COUNT          846 a         1853 c         1773 g          808 t
ORIGIN      
        1 tcgccatggg cggcaacatg ctcgacctgg cgctcggctt catcatcggg gcagcgttcg
       61 cgacgctcgt cgagagcctg gccagcaaca tcctcatgca gttggtggcg gcgatcttcg
      121 gcacgcccga cttcaccgaa ctgagcctga cgctcaacaa cgccgagatc acctacggcg
      181 cgttccttac cgacttcgtg ggcttcatcc tcatggcgct cgtgctcttt gcgatcgtca
      241 aactgctcaa gaaggccggc atgggcaact tccgggccca gggccagcgc gagtgcccga
      301 tgtgcaagga gttcgtcgcc gtcgacgcgc tcaagtgcaa gtggtgcacc gctgacatcg
      361 acccggacca ggccgaggag gaggacctcg agatcatcga ggcccgacgc aagcccgagc
      421 agccttgagc gcggactacg cgttccgcgc gccgttgtgg cggtggcaag gcgagagccc
      481 gtggcacttc gtcacgctgc ccgaggagca gtccgacgag atcgacgagc gcacccgtga
      541 cgtccaacgg ggcttcggct cgatccgggt cgaggtgacc gtcgggacga cggtctgggc
      601 gacctcgttg ttcccgtcga ccaaccatgg ggcctacctg ctgcccgtca agaaggccgt
      661 ccgggtcgcc gagggcctga cggccgacga gcccgtcgac gtgcggctgc ggttgctcga
      721 tctgccgggc ggagcaccgg gctcacgcgg ttaaccgttc ggcgacgtcc ccgcactcac
      781 gtagaatcat tggcagcggc gacgacccgg ccatcaccgg cgagcctccg gaagaaccgt
      841 ccacctccac gcgggggtgg gctcagtaga accggacggg tggcccgtca cagccggaac
      901 gacaagagcg gtcgtatgcc gcgtgctccc caccgggcga aacccgggcg gtgcgcgggc
      961 ggcatacgac aagcgaggtg gtaccgcggc aactccggtg gtgatcgacg tgatcaccgt
     1021 gatccgggag cgtcgtcctc gtggcacgta ggagccgggc gaccggcata ccggaccgag
     1081 agacgaggca ggcagaccag tggtctaccc caaggcgagc accgaccccg aggcaacggg
     1141 cgtgcccccg accccccgat tcccgacgat cgaggagcgg gtcctggcct attgggccaa
     1201 ggacgggacg ttcctcgcga gcgtcgaggc gcgcgacggc ggcaccgacg ggtccaacga
     1261 gttcgtcttc tacgacgggc ctcccttcgc caacggcctg ccgcactacg ggcacctgct
     1321 gaccggctac gtcaaggacc tcgtcccgcg ctaccgcacg atgcggggcc gacgggtcga
     1381 gcgccgcttc gggtgggaca cccacgggct gcccgccgag cttgaggcgc agcgccagtt
     1441 ggggctcaag accacgcagg agatcaccga cctggggatc gccaccttca acgacgcctg
     1501 ccgcaactcg gtgctgcgct acacgaagga gtgggaggag tacgtcaccc gccaggcccg
     1561 gtgggtcgac ttcgagcacg actacaagac tctcgacacc gactacatgg agtcggtcat
     1621 ctgggccttc aagcaattgc acgagaaggg cctggcctac gaaggcttcc ggatcctgcc
     1681 ctactgctgg aacgaccaga ccccgctgtc caaccacgag ttgcggatgg acgacgacgt
     1741 ctaccagatg cgtcaggacc cggcgatcac cgtcggctac cggctcgaga ccggcgagct
     1801 ggccctcatc tggacgacga ccccgtggac cctgccgtcg aacatgggcg ttgccgtgca
     1861 ccccgacgtc gactatgtcg tcgtgcaggg tgacaaggac ggcggcagcg accgttacct
     1921 cctcgcgcag gcgcggctcg cggcatacac ccgcgagctc ggggacgacg cggccgagcg
     1981 gatcgtcgcg cgctacaagg gatcagagct ggtcgggcgc cgctacacgc cgccgttcac
     2041 ctactacctg gggtatgccg gcgcgcacca gatctcggcg gcgggcttcg tcacgaccga
     2101 ggacggcacc gggctggtcc acatgagccc cggcttcggc gaggacgaca aactcgccct
     2161 cgacgccctc ggggtggaga ccgtcgtgcc ggtgggcccg gacgggtgct tcgtcttccc
     2221 ggtgaccgac tacgccgggt tgcacgtctt cgatgccaac cacccggtca tcgaccacct
     2281 caaggcccgc acccgcgggc acggtgagat gggctccacg accgaaggca ccgtcctcgt
     2341 tcgacacgag acctacgacc actcctaccc gcactgctgg cgctgccgcg aaccgctgat
     2401 ctacatggcg gtctcgtcgt ggttcgtcgc gacgacggcg atccgcgacc ggatgctcga
     2461 gctcaaccag gagatcacct gggtgcctga gcacaccaag gacggccagt tcggcaagtg
     2521 gctggagaac acccgcgact ggtcgatctc ccggaaccgc ttctggggca gcccgatccc
     2581 ggtgtggaag tccgacgacc ccacctaccc gcgagtggat gtctatggct cgttcgctga
     2641 gttggagcgc gacttcggcg tgcctgtcac cgacttgcac cggccgttca tcgactcgct
     2701 cacccggccc aaccccgacg acccgcgccc cgagggggag cgctcgacga tgcgccgggt
     2761 caccgacgtc ctggactgct ggttcgagtc ggggtcgatg agcttcgccc aggtgcacta
     2821 cccgttcgag aacgcggact ggttcgagca ccacttcccc ggcgacttca tcgtcgaata
     2881 catcggccag acgcggggct ggttctacac catgcatgtc ctggcgacgg cgttgttcga
     2941 ccgcccggcg ttcaagacct gcatctcaca cggcatcgtc ctgggctcgg acggacagaa
     3001 gatgagcaag agcctgcgca actaccccga cgtcttcgag gtcttcgacc gcgacggcgc
     3061 cgacgcgatg cgctggttcc tcatgtccag cccgatcctt cgcggcggca acctcgtcgt
     3121 caccgagggc ggcatccgcg acggcgtgcg ccaggtgatg atcccgctgt ggaacgcctg
     3181 gcacttcttc gcgctctacg ccaacgctgc cggcgggggc aaggggtatg ccgccacggc
     3241 ctccaccgcg tcgacggacc tgctcgaccg ctacctgttg gccaagctgc gcgagttcgt
     3301 cgaggcgatg acggcgcagt tggacagcta cgacatcgcg gctgcctgcg acaccatgcg
     3361 cagcttcctc gacgtcctga cgaactggta catccgccgc tcgcgggacc gcttctggga
     3421 cagcgacaac tcgctcgatg cctcgacgac ggccgcgttc gacacgctct acaccgcgtt
     3481 ggaggtgctc acccgcgcgg tcgccccgtt gctgccgctg acgaccgagg agatctggcg
     3541 cggcctcacc gggggtcgct cggtccacct cgaggactgg ccggacgccg ccgcgctgcc
     3601 cgccgaccac gccctcgtcg ctggcatgga ccgcgcccgg gaggtgtgct ccgcggtgtc
     3661 gtcgttgcgc aagggcgcga gcctgcgggc ccgcctgccg ctggccgagt tggtcgtcgt
     3721 cacgaccgat ccggctgcgc tggagccctt ctcggcgatc atcgccgacg aggtcaacgt
     3781 ccggtcggtg gtcctgcgcg acgtggagtt ggcgcacgag agcgacttcg gcgtgatgcg
     3841 ccgcctgtcg gtcaacgccc gggtcgccgg tccgcgcctc ggcaaggagg tccagaccgc
     3901 gatccgcgcc gccaaggcgg gggactggtc gacggccccg gatggcaccg tcaccgccgg
     3961 gggcatcgcc ctcgtggacg gcgagtatgc cgtcgagacg gtcgtcgccg gtcaggacgc
     4021 caggaccgcc gggtcaactg accagggctc cggtccctcg cacgcggtcg ccgtgctccc
     4081 ggccggcggg ttcgtcgtcc tcgacacgga ggtcaccccc gagttggccg tcgaaggtgt
     4141 cgccaacgac ctggtccggg ccgttcagca gacccgccgt gagtcgggcc tgcacgtcag
     4201 cgaccggatc tccctgtcgc tggcgggcgc tgaactcatg caacgggccg ctcacgagca
     4261 ccgggaccgg ctcatggccc agaccctcgc cgagcacctg agcgtcgcca cccatgtcga
     4321 ggacctggct ctcggtgacg gcgtcgtcga gacgtcggtc ggcgacgccc tgcccgtccg
     4381 gatcaaggtg gccaagcgct gatgggtgtc gtgatccgac cggcaacggc atacgacgtc
     4441 gacgcagtcg tcgacgtcgg tcggcgcacc tggccgcaga cctacacccc gctcgccggt
     4501 gaggattacg tgcggatggg gctggacaag tggtggaccg tggccgccac ccggccattg
     4561 gtcctcgccg gcaaggcgac tgtcgccgag ttggacggtg aggtcgtcgg cgtcgccgtc
     4621 gtggggccgc tcacgggcga cctcgtcctg ttccggctct acgtcgtccc cgaacaccaa
     4681 gggatgggga tcggccggtt gctcctggaa gacgtcatga cgaccgcccg cgagacgggg
     4741 caccggatcc tgcggttgtc ctacctggac ggaaacgccg gcgccgagcg cttctaccaa
     4801 ggcttcggct tcgtcgagtc ccatcgggaa ccgaccgggg acggcatacc cgactcgatc
     4861 tgggtcgtgc gcgacctgtg ggagcaggag cagtcgtgac cccgcccccg agcgatcccg
     4921 cggtggccgc ccgcctggct caggtggagg ccgagatcat gggccgcgcc cccgaacacg
     4981 acctcgtgcc gaccctcgat cgggtcgcgg cggtcatgga attgctgggc gacccgcagc
     5041 gcagcttccg ggtcatccac ctgaccggca cgaacggcaa gacctcgacc acccgcatga
     5101 tcgaggctct gtgccgcgaa cacggtttgc gcacaggccg attcacctcc ccacacctgc
     5161 actccatgcg ggagcggatc gccgtcgacg gtgagcccgt gtcggctgag gtgctggtgg
     5221 agacctggga cgaggtggcc ccgatcatcg agatcgtcga cgcgcgttcg gctgccgagg
//
LOCUS       DAORPW010000158         5260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4424_length_5260_cov_1007.911047, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000158 DAORPW010000000
VERSION     DAORPW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4424_length_5260_cov_1007.911047"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLB86_10645"
     CDS             complement(<1..446)
                     /locus_tag="PLB86_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRC65638.1"
                     /translation="MSWDHLDESDVRVRPGRGSRPRTKDRPAHGDALVGTVVTVDRGR
                     YGVLMAAGVGGRKVPERMVVAMKARELGRKGIVVGDDVGLVGDSSGHPDALARIVRVE
                     PRRTVLRRSADDTDPIERVLVANADQLVVVSSLTDPPPRPRLIDRCL"
     gene            complement(443..1813)
                     /gene="aroA"
                     /locus_tag="PLB86_10650"
     CDS             complement(443..1813)
                     /gene="aroA"
                     /locus_tag="PLB86_10650"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRC65639.1"
                     /translation="MSIDVDQQWWPAPVAPGPLDAVVRLPGSKSLTNRYLVLAALADG
                     PSRLRAPLRSRDTLLMAAALGTLGADIRDTGALNAVAPESSGERAAYPVSDWVVTPGA
                     VRGGGHLDCGLAGTVMRFLPPVAALADGPVAFDGDAGARRRPMGPVLDALRALGVGVD
                     DDGSGCLPFTVQGRGRDGVPGGEVTLDASASSQFVSALLLAGARYDRGVTIHHDGKPV
                     PSQPHIEMTVQTLREAGVVVDDSDANTWRVAPGSIRGLDVVIEPDLSNAGPFLAAALV
                     AGGTVLVPDWPERTTQAGDLLREILAAMGARVQLGSDGLRVTGRGPIEGIDIDLHEAS
                     ELTPTVAALAALAGSPSWIRGVAHIRGHETDRLAALDTELSALGTQVTQTEDGLRIVP
                     APLRGNRFGTYHDHRMATAGAILGLRVPGVLVEDVATTAKTLPDFTGRWTAMLGASHG
                     GAEGNG"
     gene            1891..2727
                     /locus_tag="PLB86_10655"
     CDS             1891..2727
                     /locus_tag="PLB86_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRC65640.1"
                     /translation="MCGRYAAAKDQADLVLEFDVEVVATAEPSRRVLVKPQDPPAGAP
                     DYNVAPSKQAPVVLTRASRSDPEGPSSRQLRLLTWGLVPSWAKDVKIGMRFINARAET
                     ALESGPFARAALARRCLVPADGWYEWQASPTVLDAKGKPRKQPFFIHRGDAAPLAFAG
                     LYEFWRDPAVPDRDDPLAWLTTFTIITTAAEPGLDRIHDRQPLVLEPDNWAAWLDPAL
                     TDPAAVERMLRFERAGRFAAYPVSTAVNGSAGNGAHLLDALPAAALRGVVDPTTGEII
                     GS"
     gene            2724..3413
                     /locus_tag="PLB86_10660"
     CDS             2724..3413
                     /locus_tag="PLB86_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65641.1"
                     /translation="MTSTQPLTPASLNPAPTVLEVPTSVGPARVHLRRARGSRGVLLL
                     GHGAGPSITTTDLIATTEAAVGAGWAVALVEQPWLVAGGRVAARPPILDTAWVSVAAA
                     LRARGGALATARGPWLVAGRSAGARVACRTALSVGADAVLCLSFPLHPPGKPESSRAQ
                     ELEGALADGLPVSVVQGERDPFGTPAEVLAAVSLPPTRMYAVPGTHTIPRGSAAAVGA
                     AITEVLRGIPG"
     gene            3517..4302
                     /locus_tag="PLB86_10665"
     CDS             3517..4302
                     /locus_tag="PLB86_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC65642.1"
                     /translation="MPVATRGSVAAGSHTGPLLWGAMTTDPASPTPPDATGSLAASST
                     EVLPETDLPVVADAAPALDLATETTEERRARFEREAMPLLDQLYSAALRTARNAADAE
                     DLVQETYAKAWAAFHQYQPGTNLKAWMYRILTNTYINSYRKKQREPLQSDAAEIEDYQ
                     LHRADSHSSAGGRSAEDVALDHLPDSDVKRALLAIPEEFRLAVYFADVEGYAYKEIAE
                     IMQTPIGTVMSRLHRGRRQLRDLLTEYAAERGYVRTDVQTEGR"
     gene            4461..4703
                     /gene="rsrA"
                     /locus_tag="PLB86_10670"
     CDS             4461..4703
                     /gene="rsrA"
                     /locus_tag="PLB86_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HRC65643.1"
                     /translation="MDCSEALLRAFEFLDGEMEANDFAKVRAHLDACAECLRQYDLDH
                     MVKLMIKRSCAPEPAPTALRATIVSRLTVIRIETTN"
BASE COUNT          780 a         1907 c         1791 g          782 t
ORIGIN      
        1 aggcaacggt cgatcaaccg gggccgcggt ggcgggtcgg tgagggatga gacgacgacg
       61 agttggtcgg cgttggcgac gagcacccgc tcgatggggt cggtgtcgtc ggcgctgcgc
      121 cgcaacacgg tgcggcgcgg ctcgacgcgc acgatccggg cgagggcatc ggggtgtccg
      181 gaggagtcgc cgaccagccc gacgtcgtca ccgacgacga ttcccttgcg ccccaactcg
      241 cgggccttca tcgcgacgac catgcgttcg gggaccttgc gccccccgac cccggccgcc
      301 atgagcacgc cgtagcggcc tctatcgacg gtgacgacgg tgcccaccaa ggcgtcgccg
      361 tgggccggac ggtccttcgt ccgtggccgc gacccgcgac ccgggcggac tcgaacgtcc
      421 gactcgtcga ggtggtccca gctcacccgt tgccctcggc tccgccgtga ctcgcgccca
      481 acatcgccgt ccaacgtccg gtgaagtcgg gcagcgtctt ggccgtcgtg gcgacatcct
      541 cgacaaggac acccgggacg cgcagcccga gaatggcccc ggcagttgcc atacggtggt
      601 cgtggtaggt gccgaaccga ttgccgcgca acggtgccgg aacgatccgt aacccgtcct
      661 cggtctgggt cacctgggtg ccgagggcgc tcaactcggt gtcgagggcg gccagccgat
      721 cggtctcgtg cccgcggatg tgggcgactc ctcggatcca cgatggcgac ccagccagtg
      781 cggcgagcgc ggcgacggtg ggggtgagct cgctggcttc gtgcaggtcg atgtcgatgc
      841 cctcgatcgg cccgcggccc gtcacccgca gcccgtcgga gcccaactgc acccgcgccc
      901 ccatggcggc gaggatctcg cgcagaaggt cgcccgcctg tgtcgtgcgc tccggccagt
      961 cgggcacgag caccgtcccg ccggccacga gggcggcggc aaggaacggc ccggcattgg
     1021 acaggtcggg ctcgatgacg acatccagac cgcggatcga ccccggcgcc acccgccagg
     1081 tgttggcgtc ggagtcgtcg acgaccaccc cggcctcgcg cagcgtctgc accgtcatct
     1141 cgatgtgcgg ctggctcggc accggcttgc cgtcgtgatg gatggtcacc ccgcggtcgt
     1201 agcgggcgcc cgccagcaac agcgccgaca cgaactgcga gctcgccgag gcatcgaggg
     1261 tgacctcgcc gccgggcacc ccgtcgcgcc cccgcccctg gacggtgaac ggcagacagc
     1321 ccgagccgtc gtcgtccaca ccgacgccca gggctcgcaa ggcgtcgagg accggaccca
     1381 tcggtcgtcg ccgcgcaccc gcgtcgccgt cgaaggccac cggcccgtca gccagagccg
     1441 ccaccggggg aaggaaacgc atgacggtgc cggccagtcc acagtcgagg tgcccgcccc
     1501 cgcgcaccgc acctggggtg accacccagt cgctcaccgg gtatgccgcc cgctcaccgc
     1561 tgctttcggg tgccactgcg ttgagggcgc cggtgtcgcg gatgtctgct ccgagagtgc
     1621 cgagggccgc ggccatgagc agcgtgtcgc gagatcgcag cggggcccgc aaccgcgaag
     1681 gcccgtcggc caacgcggcc aacacgagat agcggttggt cagcgacttg ctgcccggca
     1741 ggcgcacgac agcgtccaac ggcccgggcg ccacgggcgc aggccaccac tgttggtcga
     1801 catcgatgct cacccgacaa ccgtagttgg tcgcaccctg gaggctgctg caactgcgtg
     1861 ccgtcgggag cgcggcatac cctgtctctc atgtgtggga ggtatgccgc tgccaaggac
     1921 caggccgacc tcgtgctgga gttcgacgtc gaggtggtcg ccacggccga gccctcgcgc
     1981 agagtcctgg tcaaaccaca ggatccgccg gccggtgccc ccgactacaa cgtcgcgcca
     2041 agcaaacagg cgcccgtcgt cctcacccgg gcgtcgcgct cggacccaga aggtccctcg
     2101 agccggcaac tgcgactgct cacctggggc ctggtgccca gttgggccaa ggacgtcaag
     2161 atcgggatgc ggttcatcaa cgcccgcgcc gagaccgcgc tcgaatcggg cccgttcgcc
     2221 cgggccgcgc tggcccggcg gtgcctcgtc cccgcagatg gttggtatga gtggcaggcg
     2281 tcgccgacgg tgctcgacgc caaaggcaag ccccgcaagc agcccttctt catccatcgg
     2341 ggagatgcgg cgcccctcgc tttcgccggt ctctatgagt tctggcgcga ccccgcggtc
     2401 cccgaccggg acgacccgct cgcctggctg acgaccttca ccatcatcac gaccgccgcg
     2461 gagcccggcc tggaccggat ccacgaccgc caaccgctcg tcctggagcc ggacaactgg
     2521 gccgcctggc tcgaccccgc cctgaccgac ccggccgctg tcgagcggat gctgcgcttc
     2581 gagcgggccg ggaggttcgc ggcatacccc gtctcgacgg cggtcaacgg gtcggcgggc
     2641 aacggcgcgc accttctcga cgccctcccg gcggccgcgc tgcgcggggt cgtcgacccg
     2701 acgaccggcg agatcatcgg ctcatgacat cgacccagcc gctgactccg gcgtcactga
     2761 acccggcgcc cacggtcctg gaagtgccca cctccgtcgg tccggcgcgg gtgcacctgc
     2821 gccgggcgcg cggctcccgt ggggtgctcc tgctcggtca cggggccggc ccgagcatca
     2881 ccaccaccga cctcatcgcc accaccgagg ccgccgtcgg cgcgggttgg gcggtcgcgc
     2941 tcgtcgaaca accgtggctc gtcgcaggcg ggcgggtcgc tgcgcggccg cccatcctcg
     3001 acacggcctg ggtgtcggtc gccgctgctc tgcgggcccg cggcggggcg ctcgcgaccg
     3061 cgcgcggccc ctggctggtc gccggccgaa gcgcgggcgc gcgggtcgcc tgccggaccg
     3121 ccctcagcgt cggcgccgac gcggtgctgt gcctgagttt cccgctgcac ccgccgggca
     3181 aacccgaatc gagccgcgcc caggagttgg agggcgcgct cgccgacggg ctcccagtgt
     3241 cggtcgtgca aggcgagcgc gaccccttcg ggaccccggc cgaggtgctc gccgccgtgt
     3301 cgctcccacc gacccggatg tatgccgtcc ccggcaccca cacgatcccg cggggctcgg
     3361 ccgcagcggt cggtgcggcg atcaccgagg tgctgcgcgg catacccggc tgaggaggct
     3421 gggggagtga actgggaatg aacgatgccg tcgggggcgt tgcacgtcac gatccgcaac
     3481 cacccccggg aagctaggag catcgtcgtg ttcgtcatgc cggtcgccac cagaggttcg
     3541 gttgctgccg gcagtcacac gggaccgtta ctgtggggcg cgatgaccac cgatcccgcg
     3601 tccccgaccc ctcccgacgc caccggctcg ctcgccgcct cgtccaccga ggtgctgccc
     3661 gagaccgacc ttcccgtcgt cgctgacgct gcgcctgccc tcgacctggc caccgagacc
     3721 accgaggagc ggcgcgcgcg cttcgagcgc gaagccatgc cgctgcttga ccagctctac
     3781 tcggccgcgc tgcgcaccgc gcgcaatgcc gctgacgccg aggacctcgt ccaggagacc
     3841 tacgccaagg cctgggccgc gttccaccag taccagccgg ggaccaacct caaggcgtgg
     3901 atgtaccgca tcctcaccaa cacttacatc aactcctacc gcaagaagca gcgcgaaccg
     3961 ctgcagtcgg atgcggccga gatcgaggac taccagctgc accgggccga ctcgcactcc
     4021 tcggcgggcg gtcggtcggc tgaagacgtg gcgttggatc atctgcccga cagcgacgtc
     4081 aagcgcgccc tgctcgcgat tcccgaggag ttccggttgg cggtctattt cgccgacgtc
     4141 gaggggtatg cctacaagga gatcgccgag atcatgcaga cccccatcgg gaccgtgatg
     4201 tcacggctac accgcgggcg ccggcaattg cgcgatctgc tgaccgagta tgccgccgag
     4261 cgcggctacg tccgtaccga cgtccagacc gaggggcgat gatgaccagc gagcaccgtg
     4321 agcccggtcc gctcggctcc agttcggtgg gtccgcgatc cgcgggccct gcggccacgc
     4381 ccgctggggc gttcgtctcg gagtccgcct cggggtccgt cgaccctgga catcaccatc
     4441 cgcatggcgg tccgacggcg ttggactgtt cagaggcgtt gctgcgagcc ttcgagttcc
     4501 tcgacggcga gatggaggcg aatgacttcg ccaaggtgcg ggcacatctg gacgcgtgcg
     4561 ccgagtgcct acgtcagtac gacctcgacc acatggtcaa gttgatgatc aagaggtcgt
     4621 gtgcgccgga gcccgccccc acggcgctgc gcgcgaccat cgtcagccgc ctgaccgtca
     4681 ttcggatcga gacgaccaac taggtcgcgc gcgggtgcgg ccgctcaggc gttgggcttg
     4741 cgcccgtggt tggcggcgtg gctcttgcgg gaacgccgct tgcgggaacg cttgctcatg
     4801 gtgactccag ggtgtttgcg atgcggtctt cggtagttgg tgtagcccgc gaatgatagc
     4861 ggttcctgcc gaggccgcgt ccgcggtttc gactggaatg tccgagttcc gtggcgcaca
     4921 ctaaggggca gacgagtaca cctcaggctc gtgtacggct tcagtagccg actctcccgg
     4981 acttcgagga tcgtatgaag gccatccaac gcctgtttgt ggcgatgttg acccaggacg
     5041 tcactccgtt gtggggacct gcgtccgcac cactgccatg acgcagtggc gtgctcaccc
     5101 ctgccgctgt cagtacgcct gccgtcacct ggccatgcgg atgccgacca gcgatacccc
     5161 ttttcaatac caccgaaagt caaaggaact gacatgaaga ctgtctccaa gttgttcgaa
     5221 gccatgctga ccaaggagac gaccccggtg tggggtggcc
//
LOCUS       DAORPW010000159         5235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4460_length_5235_cov_652.044015, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000159 DAORPW010000000
VERSION     DAORPW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4460_length_5235_cov_652.044015"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..638)
                     /locus_tag="PLB86_10675"
     CDS             complement(3..638)
                     /locus_tag="PLB86_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HRC65644.1"
                     /translation="MTFSIAIAAAAFALIFPVEFPDKTFVATLVLATRFRPLLVWIGV
                     ASAFLVQTVVAVLAGQLLANLPRTPVEIASGLLFLIGGVILLRGAAKADDEEAEAEAE
                     FTEVAASKAGATGVKAVATSFLVLFLAEWGDLSQLLTANLVVRYGEPLSVGVGAFLAL
                     ATVSGLAAALGHQLLRRVKLATIRRIGGGVCLALAALSVLGLFGVDVPILG"
     gene            714..1631
                     /gene="pheA"
                     /locus_tag="PLB86_10680"
     CDS             714..1631
                     /gene="pheA"
                     /locus_tag="PLB86_10680"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRC65645.1"
                     /translation="MTRYGFLGPEGTFCEMALRQWAASHEGDHHPFGSVGAALRALRE
                     GRIDAAMVPIENSVEGGVSATLDALSVGEQLHIIGEVLVPITFVLAARPGMPLSRVRA
                     VGTHSHAWAQVRGWMDANLPDAAYLPTLSTAASAQTLATETDPPFEAAVCAPGAATRF
                     GLDVLAEDIGDNGGAVTRFVLVSRPGHLPPASGADKTTVVLFQKLDRPGGLLELLEQF
                     AVRGVNMVRLESRPTGEAMGSYCFSIDFEGHLAEERVRESLIGLKRVCADVRFLGSYP
                     RADGRQGIIDAGMGDDDFVVARAWIDALS"
     gene            1736..2596
                     /locus_tag="PLB86_10685"
     CDS             1736..2596
                     /locus_tag="PLB86_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HRC65646.1"
                     /translation="MFFSNALIGLREGLEAALVVVILIAFLVKTDRRWALRYVWIGVG
                     AAVALSVVIGAVLTFGTSGLDDRTAELVGGLASLLAVVLVTGMIFWMRSVGRRFAGEL
                     TGKLDRALDAGPFAIAAVAFLGVGREGLESALFFYATVESAGETGIAPALGWVVGIGA
                     AVALGIAIYLGAVRIDLAQFFRWTGVGLVIVAAGILAYAVHELQEAGVLPGENVYAFD
                     LRSTIDPTSPLAMVVRGIFNLRPRMAVLEIGAWAAYLVVVLPLFLRGVGRRPTATAAA
                     AAESVPAAKR"
     gene            complement(2593..3693)
                     /locus_tag="PLB86_10690"
     CDS             complement(2593..3693)
                     /locus_tag="PLB86_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRC65647.1"
                     /translation="MPENLLWWIVGAVVLFAGIAVAAAMVNQGVVDYDALTQRLRRQR
                     PHRDSFRDTPVVSDEPPRRAAVIVNPTKVDDLSEVRARIAGVCAAHGWGPPIWAETTA
                     EEPGTAQARQAVLDGAVLVCPLGGDGTVRAVAKALVDTETPLGLLPGGTGNLLARNLD
                     LPLDSVEKALVVALTGHNKSVDVGQIIIDRGNGAEPEEDTFLVMAGIGFDAAVMAGAP
                     EHLKRQVGNAAYILAGLRNMKGPQFKARMSVDGGMDFTRRTRTVLLGNCGKIFGGLVL
                     MPEAKIDDGLIDAVAMSPKGIVGWTAVLARIVSRQRKGHPIVDHHTGREIRVRMDRPE
                     ELQLDGDTLGPVRSVTASVRSGALVVRVAGSG"
     gene            3772..4305
                     /locus_tag="PLB86_10695"
     CDS             3772..4305
                     /locus_tag="PLB86_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC65648.1"
                     /translation="MAPKPQAPPPDDKDWTWVLTQPCQECGFDPATIRSKRLADAVRE
                     TASRWPAVLVQPAVAERPSKAVWSPLEYACHVRDVMRLFAQRIELMLAEDNPTFANWD
                     QDRTAREDRYWDQDPAKVAKQIGQAGDKLADLFERVSKEDWQRRGTRSNGSEFTVATI
                     GVYALHDLRHHVWDVRG"
     gene            4423..>5235
                     /locus_tag="PLB86_10700"
     CDS             4423..>5235
                     /locus_tag="PLB86_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRC65649.1"
                     /translation="MIDIKLVRDNPDVVRRSQQARGEDPAIIDAILAADETRRSSLAQ
                     FEAARAEQKSFGKQVAAAAPADKPALLAQVKDLAERVKALDAAAATAGEDAVRLLASV
                     PNVVLDGVPTGGEEDYVVRETVGTPRDFAAEGFEPRDHVALGESLGAIDIERGAKVAG
                     ARFYFLTGVGARLELALLNLAVARAVDAGFTPMITPTLVRPEIMRGAGFLDHHSDEVY
                     YLPADDLYLTGTSEVALSGYHRDEIIDLAEGPKRYAGWSACYRREAGSHGKDT"
BASE COUNT          762 a         1890 c         1797 g          786 t
ORIGIN      
        1 cctcagccga ggatcggcac atccaccccg aacaggccga gcacgctcaa ggcggccagc
       61 gccaggcaga ccccgccacc gatgcggcgg atcgtcgcca acttcacccg gcgcagcaac
      121 tggtgcccca acgccgccgc gagccccgac acggtcgcca gcgcgaggaa cgccccgacg
      181 ccgaccgaca acggctcgcc ataacggacg acgaggttgg ccgtcagcag ctgcgacagg
      241 tcgccccatt cggcgaggaa cagcacgaga aaactcgtcg cgacggcctt gacccccgtg
      301 gccccggcct tgctcgcggc gacctcggtg aactcggcct ccgcctcggc ctcctcgtcg
      361 tcggccttcg ccgccccgcg cagcaggatg accccgccga tgaggaacag caggcccgac
      421 gcgatctcca cgggcgtccg cggcaggttg gccagcagtt gcccggcgag cacggcgacc
      481 accgtttgca ccaggaacgc gctggccacg ccgatccaca cgaggagcgg tcggaagcgg
      541 gtcgccagca cgagcgtcgc gacgaacgtc ttgtccggga actccaccgg gaagatgagc
      601 gcgaacgcgg cagcggcaat ggcgatactg aatgtcataa cgagcatcga gcgtaatacc
      661 ctgccgaacg tgcccactcg ccagcccaac cgacgtcccg aggcgtggac ccgatgaccc
      721 gctacggctt cctcgggcct gaggggacct tctgcgagat ggccctgcgg cagtgggcgg
      781 cctcgcacga gggtgaccac catccgttcg gctcggtcgg tgccgccctg cgcgccttgc
      841 gcgagggccg catcgacgca gccatggtgc cgatcgagaa ctcggtcgag ggcggcgtca
      901 gcgcgacgct cgacgccctg tcggtgggtg aacaactgca catcatcggc gaggtgctcg
      961 tccccatcac gttcgtgctc gccgcccgac cgggtatgcc gttgagccgg gttcgcgccg
     1021 tcggcaccca ctcgcacgcc tgggctcagg tgcgcggctg gatggacgcg aacctgccgg
     1081 acgcggcata cctgcccacc ttgtcgaccg cggcctcggc gcagacgctg gccaccgaga
     1141 ccgacccgcc cttcgaggcg gcggtgtgcg cccccggagc ggcgacccgc ttcggcctgg
     1201 acgtcctcgc cgaggacatc ggcgacaacg gtggcgcggt gacccgcttc gtcctcgtca
     1261 gccggccggg gcacctgccg cccgcgagcg gggccgacaa gacgaccgtg gtgctgttcc
     1321 agaagctcga ccggccgggt ggcctgctgg agttgcttga gcagttcgcg gtccgcggcg
     1381 tcaacatggt gcgcctggag tcgcgaccca ctggcgaggc gatgggttcc tactgcttct
     1441 cgatcgactt cgaggggcac ctcgccgagg agcgcgtccg cgagtcgctc atcggcctca
     1501 agcgggtctg cgccgacgtg cgattcctcg gttcctaccc gcgcgccgac ggccgccagg
     1561 ggatcatcga cgcggggatg ggtgacgacg acttcgtggt cgcccgcgcc tggatcgacg
     1621 ccctctcgtg actccaacgc tcgggatgcc gcccaggacc gcagttgtta ggtaaccctt
     1681 acctgtgcca tcctgaacag gtaaggcgta cctaactttc ctcctaagga gtgccgtgtt
     1741 cttctccaac gccctcatcg gcctacgcga gggtctggaa gcggcgctcg tcgtcgtcat
     1801 cctcatcgcc ttcctcgtca agaccgaccg tcggtgggcg ctgcggtacg tctggatcgg
     1861 cgtgggcgcc gcggtcgcgc tctcggtggt gatcggcgcc gtcctcacct tcggcaccag
     1921 cgggctggac gatcgcaccg ccgagttggt gggtgggctg gcctcgctgc tggccgttgt
     1981 cctggtcacc ggcatgatct tctggatgcg ctcggtgggg cggcggtttg cgggtgagtt
     2041 gaccgggaag ctcgaccgag ccctggacgc cggcccgttc gcgatcgccg ccgtggcctt
     2101 cctcggcgtt ggccgcgagg ggctggagtc ggcgctcttc ttctatgcca ccgtcgagtc
     2161 cgccggtgag accggcatcg cccccgcgct cggctgggtg gtcgggatcg gcgccgcggt
     2221 cgccttgggc atcgcgatct acctcggggc ggtgcggatc gacctcgcgc agttcttccg
     2281 gtggacgggc gtcggcctgg tgatcgtcgc ggccggcatc ctcgcgtatg ccgtgcacga
     2341 actgcaggag gccggcgtgc tcccgggcga gaacgtctac gccttcgacc tgcgctcgac
     2401 catcgacccg acctcgccgt tggccatggt cgtccgcggc atcttcaacc tgcggccacg
     2461 gatggccgtg ctcgagatcg gggcgtgggc ggcatacctc gtggtcgtgc tgccgctgtt
     2521 ccttcgcggg gtgggccgtc gtccgacggc gaccgcagcc gccgcagccg agtcggtccc
     2581 ggccgcgaag cgctaaccgc ttccggcgac ccgcacgacg agcgcacccg agcggaccga
     2641 cgcggtcacc gatcgcaccg ggccgagggt gtcgccgtcg agttgcagct cttcgggccg
     2701 atccatccgc acccggatct cgcgaccggt gtggtgatcg acgatggggt gacccttgcg
     2761 ctggcggctg acgatgcggg cgagcacggc ggtccagccg acgattccct tgggagacat
     2821 ggcgaccgcg tcgataaggc cgtcgtcgat cttggcctcg ggcatgagca ccaagccgcc
     2881 gaagatcttg ccgcagttgc ccaacagcac ggtccgggtg cgtcgggtga agtccatccc
     2941 accgtcgacg ctcatgcgcg ccttgaactg cgggcccttc atgttgcgca ggcccgcgag
     3001 gatgtatgcc gcgttgccca cctgccgctt gaggtgctcc ggcgcccccg ccatgaccgc
     3061 cgcgtcgaag ccgatgccgg ccatgacgag gaaggtgtcc tcctccggct cggcgccgtt
     3121 gccgcgatcg atgatgatct ggcccacatc gacgctcttg ttgtggccgg tgagggccac
     3181 gacgagggcc ttctccaccg aatccagcgg caggtcgagg ttgcgggcga gcaggttgcc
     3241 tgtgccgccg ggcagcagcc cgagcggggt ctcggtgtcg acgagagcct tggccaccgc
     3301 gcgaactgtc ccgtccccgc cgagtgggca gaccaggacg gcgccgtcga ggacggcctg
     3361 gcgggcttga gcggtgcccg gctcctctgc cgtcgtctcg gcccagatgg gcggacccca
     3421 gccgtgggca gcgcagacgc cggcgatcct cgcgcggacc tcgctgaggt catcgacctt
     3481 ggtcgggttg acgatcacgg cggcccggcg gggcggctcg tcggacacga ccggggtgtc
     3541 ccggaagctg tcgcgatggg ggcgctggcg ccgcaggcgc tgggtgagcg cgtcgtagtc
     3601 gaccacgccc tggttgacca tggccgcagc aacggcgatg cccgcgaaga gcaccaccgc
     3661 tccgacgatc caccacagca ggttctcggg cacccaccga ccgtatacga cgccaccaac
     3721 tcgactcgct cgaccccgcg gcctgacggg gcttgacggc atacttcatg catggccccg
     3781 aaaccccaag cgccgcctcc cgacgacaag gactggacct gggtgctcac ccagccctgc
     3841 caggagtgtg gcttcgaccc ggcgaccatc cgctccaagc gcctggccga cgcggtccgg
     3901 gagaccgcct cacgctggcc ggcggtgctc gtgcagccag ccgtcgccga gcgtccgtcc
     3961 aaggcggtgt ggtcgccgct ggagtacgcc tgtcacgtcc gcgacgtcat gcggttgttc
     4021 gcccagcgga tcgagttgat gctcgccgag gacaacccga cgtttgccaa ctgggaccag
     4081 gaccgcacgg ctcgcgagga ccgctactgg gaccaggacc cggcgaaggt cgccaagcag
     4141 atcggtcagg ccggcgacaa actggccgac ctgttcgagc gggtgagcaa ggaagactgg
     4201 cagcggcgcg gcacccgcag caacggctcg gagttcaccg tcgccaccat cggcgtctac
     4261 gcgttgcacg acctgcgcca ccacgtctgg gacgtgcgcg gctgacccgg ccgcgctccc
     4321 actgaccgag gtggccggca taccccaggg gtatgccgcc cgctcaccgc ctgcatgaaa
     4381 ctgggccgag gcggtggccg gggcctcggt aggctggcgg gcgtgatcga catcaagctc
     4441 gtccgtgaca accccgacgt ggtccgccgc tcgcagcagg cccgcggcga ggaccccgcg
     4501 atcatcgacg cgatcctcgc ggccgacgag acccgccggt cgtccctcgc gcagttcgag
     4561 gccgctcgcg ccgagcagaa gtccttcggc aagcaggtcg ccgcggcggc gcccgccgac
     4621 aagccggccc tgctggccca ggtcaaggac ctcgccgagc gggtcaaggc gctggatgcc
     4681 gccgctgcca cggccggcga agatgccgtt cggctgctcg cgagcgtccc caacgtcgtc
     4741 ctcgacggcg tgccgaccgg aggcgaagag gactacgtcg ttcgcgaaac cgtcggcacc
     4801 ccaagggatt tcgctgccga gggcttcgag ccccgcgacc acgtcgcgct gggggagtcg
     4861 ctcggggcca tcgacatcga gcgcggcgcc aaggtcgccg gtgcccggtt ctacttcctc
     4921 accggcgtcg gggccaggct ggagctcgcg ctgctcaacc tcgccgtggc gcgggccgtc
     4981 gacgcgggtt tcaccccgat gatcaccccg accctggtgc ggcccgagat catgcgcggt
     5041 gccggcttcc tcgaccacca ctcggacgag gtctactacc tccccgccga cgacctttac
     5101 ctcacgggga cctccgaggt ggcgctctcg ggctaccacc gcgacgagat catcgacctc
     5161 gccgagggcc ccaagaggta tgccgggtgg tcggcctgct accgccgcga ggccggcagc
     5221 cacggcaagg acacc
//
LOCUS       DAORPW010000160         5209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4487_length_5209_cov_730.891929, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000160 DAORPW010000000
VERSION     DAORPW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4487_length_5209_cov_730.891929"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..1138)
                     /locus_tag="PLB86_10705"
     CDS             complement(299..1138)
                     /locus_tag="PLB86_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRC65650.1"
                     /translation="MTGFAAGNFAVVALASALAVAALMVGTAYLARRVGKVSIVDVTW
                     GLAFVAISWVALALGTGDLARRVLLAVLVTLWGARLAWHIGRRGHGAPEDPRYVALLA
                     DAPGGDIHRYAVLRVFVPQGAIAWFVGLPVQVSASVTGGDGTGGTGGTGWLGGLGVLG
                     VLGVAVWIVGFAFEAVGDAQLRRFKADPANRGRVMDRGLWAWTRHPNYFGDAMVSWGL
                     WLVAAGHWPGVLTVLSPVLMTVLLVRVSGAKLLEKAMGARPGYADYIARTSSFLPRPP
                     RRR"
     gene            complement(1135..1959)
                     /locus_tag="PLB86_10710"
     CDS             complement(1135..1959)
                     /locus_tag="PLB86_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC65651.1"
                     /translation="MPPTPRPPRRALVTGGVSGLGLALVRALAARGDQVMAADLADAA
                     PDGVLPAGVTYRQLDVRSDADWAAACEAIERDWGGLDLLVNNAGIAAGGRIEVAEMSE
                     WEATVAVNLLGVVRGCRTFTPMLKRQQSGTIVNIASLAGLTQAPAMSAYNATKAGVVA
                     LSETLLHELGPHGIQVSVVCPSFLRTNLAVSLQGADTLAEEGKERYLNRAKRTPEVVA
                     ARILRGVDAGRYLILTDADGRVGYWTKRLATPLYRRAMTVPGARMAAVERRGKTGG"
     gene            2084..3418
                     /locus_tag="PLB86_10715"
     CDS             2084..3418
                     /locus_tag="PLB86_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRC65652.1"
                     /translation="MSDTTPRDLDIVIFGATGFVGRLTAAYLAERAPAGVRIALAGRN
                     HERLEEVRAGLPDAAAGWEIIVADASEPVTLAALAARTRVVATTVGPYVRLGMPLVEA
                     CAIAGTHYCDLTGEVLFVRQSADTWHETARETGARIVHSCGFDSIPSDLGVLVTADTV
                     AADGAGELTETVLSVVSMRGGVSGGTIDSMRQQAILMRADAAVRAIVADPYGLSPDRP
                     AEPRSRGAEAGAEADAGADAGAGAGALAKTVWTSAVRLVRRSPIRRDPVTGHWTGPFV
                     MAGFNTRIVRRSNALLGWRYGRAFRYREVVDFGNSAKAPVLATGMSAGLLGLAGAMAF
                     EPTRAVVDRFLPKPGEGPSEENQANGRFRMVIRTTTTTGAAYRTKVGADRDPGYSGTA
                     VMLGESALALALDGDLLPGGGGVLTPATGLGSVLVDRLVAQGFTFDCERVDS"
     assembly_gap    3477..3576
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3577..4105)
                     /locus_tag="PLB86_10720"
     CDS             complement(<3577..4105)
                     /locus_tag="PLB86_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRC65653.1"
                     /translation="MAAPITYVLVDGENVDATLGTSILMRRPASEERPRWERLLSFAE
                     TTWSQPARGLFFLAANADLPMAFVQALLAIGYRVIPLRGAPGEKVVDLAIQRTLEEIG
                     RRSGDVILVSNDGDFIPQVSDLCSTRRVGIIGFTEFRNQGLTALSQRGVELFDLEYDV
                     QAFNSRLPRVRIIPIE"
     gene            complement(4115..>5209)
                     /locus_tag="PLB86_10725"
     CDS             complement(4115..>5209)
                     /locus_tag="PLB86_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC65654.1"
                     /translation="WWLDRGVDGFRFDVINLVSKQFNADGTGWFFNGPRIHEFVAEMH
                     ENAWGGKQLITVGEAPGATVDDAVRFTDPARGEFDMVFQFEHMGVDHGMGGRYDIRPV
                     DWVRLKSIMARWQDGLADVGWNSLYWCNHDQPRPVSRYGAGSTAYREVSAKALATVLH
                     LHRGTPYVYQGEELGMVNYPFTSLDQLDDVESRNWSAEAVGKGMDPAAALAAVNVQSR
                     DHARTPMQWDASEHAGFTTGTPWLPVNPAYREINAAAQVGDPESVFAHYQRLIRLRRE
                     LPVVVEGRFELLLADHPSVYAFTRALEGDELLVVANLSGESVEVALPDGWEDAEVVIS
                     SGDSDDSGMPPAHLGVLAPWQAWVRRRQVG"
BASE COUNT          785 a         1767 c         1830 g          727 t
ORIGIN      
        1 ctgtcatgac catggggggt caccgtccta catccgactc gaagaactga ggcccggggg
       61 gatctcagag gcgggagcgg gtcagcggtt cgcccttccg gagggttcgg aggggaccag
      121 ccggaagggc gaaccgcagt accacacacg tggtgggtgg gccagaaggg agagctggcc
      181 catccaccat gaccagcgca ccggatgcag cccacatctg gagggctagt caatgcatgg
      241 cgggtggggc ggcgggggat tgccgtccca cccgctatgc atttctgccc gcacgcgatc
      301 agcggcgccg aggcggccgc ggcaggaaac tactggtccg cgcgatgtag tcggcgtaac
      361 ccggtcgggc acccatcgcc ttctcgagca gcttcgctcc agacacccgc acgagcaaca
      421 cggtcatgag cacgggtgag agcacggtga gcacgcccgg ccagtgcccg gcggccacga
      481 gccacaggcc ccaactgacc atggcgtcgc cgaagtagtt ggggtggcgg gtccacgccc
      541 acagcccccg gtccatgact cggccacgat tggccggatc ggccttgaac ctgcgcaact
      601 gcgcatcgcc gaccgcctcg aacgcgaacc cgacgatcca cacggccacc ccgagcacgc
      661 cgagcacccc cagcccgccg agccacccgg tcccgccggt cccgccggtg ccgtcgccgc
      721 cggtgacgct cgcgctcacc tggacgggca accccacgaa ccacgcaatg gcgccctgcg
      781 ggacgaagac gcgcagcacg gcataccgat gaatgtcccc gcccggagcg tcggcgagga
      841 gtgcgacgta gcgggggtcc tcgggggcgc cgtgtccgcg ccggccgatg tgccaggcca
      901 gccgcgcccc ccagagggtg acgaggacgg cgaggagcac gcggcgggcg aggtctccgg
      961 tcccgagcgc gagcgccacc cacgagatgg cgacgaaggc caggccccac gtgacgtcga
     1021 cgatgctcac tttgcccacg cgacgggcaa ggtatgccgt gccgaccatg agcgccgcga
     1081 ccgccagggc cgaggcaaga gcgacaaccg cgaagttccc ggccgcgaag ccggtcatcc
     1141 gccggtcttc ccccggcgtt cgaccgcggc catccgcgcg cccggcaccg tcatggcgcg
     1201 gcgatagagg ggcgtcgcca ggcgcttggt ccagtagccg acccgcccgt cggcatcggt
     1261 gaggatgagg tagcggccgg cgtcgactcc gcgcaggatg cgtgcggcga cgacctcggg
     1321 ggtgcgcttg gcccggttga ggtagcgctc cttgccctcc tcggccagcg tgtcggcccc
     1381 ctgcagcgag accgcgaggt tggtccgcag gaacgacgga cagaccacag acacctgtat
     1441 gccgtgcggc cccaactcgt gcagcagcgt ctcggacagg gcgacgaccc cagccttggt
     1501 cgcgttgtag gcactcatcg ccggggcttg ggtgagcccg gcgagcgagg cgatgttgac
     1561 gatcgtgccg gactgctgcc gcttgagcat cggggtaaag gtccggcagc cccgcacgac
     1621 gccgagcagg ttgaccgcga cggtggcctc ccactcggac atctcggcga cctcgatccg
     1681 cccgcctgcc gcgatgccgg cgttgttgac caagaggtcc agaccgcccc aatcgcgctc
     1741 gatcgcctcg catgcggccg cccagtcggc gtcggagcgg acgtcgagtt gccgataggt
     1801 gacgccggcc gggagcacgc cgtcgggcgc tgcgtcggcg agatcggcgg ccatgacctg
     1861 atcgccccgg gcggcgaggg ctcggacgag ggccagcccg aggcctgaga ctccgcccgt
     1921 gacgagcgcg cgacgcggcg ggcggggggt gggcggcata ccgggacctc ctggggtgct
     1981 gcggacgtgc gggtcggcgc gagcgggagt ggtgtgaggc gtgtgcgtgg gggtgcgcgt
     2041 gggcgtggtg tgaggtcgaa cctaccggcg ctacggtggc tcggtgagcg acaccacccc
     2101 acgggacctg gacatcgtca tcttcggcgc gaccggcttc gtcggccggc tgaccgcggc
     2161 atacctcgcg gagcgggccc ctgcgggcgt gcggatcgcg ctcgccggtc gcaaccacga
     2221 gcgcctcgag gaggtgcgcg ccgggttgcc ggacgcggcg gccgggtggg agatcatcgt
     2281 cgccgacgcc tccgagcccg tgacgctggc ggcgctcgcg gcgcggacgc gggtcgtggc
     2341 gacgacggtt gggccctacg tccgactggg tatgccgctc gtcgaggcct gcgcgatcgc
     2401 gggcacgcac tactgcgacc tcaccggcga ggtgttgttc gtgcggcaga gtgccgacac
     2461 ctggcacgag accgctcggg agacgggggc gcggatcgtg cattcatgcg ggttcgactc
     2521 gatcccgtcc gacttggggg tgttggtcac ggccgacacc gtggctgccg atggggcggg
     2581 cgagctcacc gagaccgtgc tgtcggtggt gtcgatgcgc ggcggggtga gcggcggcac
     2641 gatcgactcg atgcgccagc aggcgatcct catgcgggcc gacgctgcgg tccgggcgat
     2701 cgttgccgat ccgtacggct tgagccccga ccggcccgcc gaaccgcgct cccgtggtgc
     2761 tgaggccgga gccgaggctg acgccggggc tgacgccggg gctggggccg gggcgctagc
     2821 taagacggtc tggacgtcgg cggttcgact ggttcggcgg tcaccgatcc gccgcgaccc
     2881 ggtgaccggg cactggaccg ggccgttcgt catggccggg ttcaacacgc ggatcgtccg
     2941 gcgcagcaac gcgttgctgg gctggcggta tggccgggcg ttccgctatc gcgaggttgt
     3001 cgacttcggc aacagcgcca aggctccggt gctggcgacc gggatgtcgg caggtttgct
     3061 cggcttggct ggggcgatgg ccttcgagcc gactcgggcg gtggtcgacc ggttccttcc
     3121 caagcctggc gagggaccga gcgaggagaa ccaggcgaac ggccggttcc ggatggtgat
     3181 ccgcacgacg acgaccacgg gcgcggcata ccggacgaag gtgggggccg atcgggaccc
     3241 tggctacagc gggaccgcgg tcatgctcgg cgagtcggcg ctggccttgg cgttggacgg
     3301 tgacctgctg ccggggggcg gaggcgtgct gacgccagcg accggcttgg gttcagtgct
     3361 cgtcgaccgc ctggtagccc aaggcttcac tttcgactgc gagcgcgtcg actcctgacc
     3421 ccagcctgat ctcgcccaag cctgccctac ctgacctgcc cgacccctgc cgacccnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttc gatggggatg atccgcaccc
     3601 ggggaaggcg cgagttgaag gcctgcacgt cgtattcgag gtcgaacaac tcaacccctc
     3661 gctgactcag agcggtgagc ccctggttgc ggaactcggt gaatccgatg atcccgaccc
     3721 gccgcgtcga gcacaggtcg ctcacctgcg ggatgaaatc cccgtcgttg ctgaccagga
     3781 tgacgtcgcc cgatcggcgg ccgatctcct cgagggtccg ctggatggcc agatcgacga
     3841 ccttctcgcc gggggcgccg cgcaacggaa tgacgcgata gccgatggcc agcagcgcct
     3901 gcacgaacgc catcggcagg tcggcattgg cggcgaggaa gaacaacccg cgggccggct
     3961 gagaccacgt cgtctcggcg aagctcagca gccgctccca gcgaggacgt tcttcacttg
     4021 ccggacgccg catgaggatc gacgtcccca acgtagcgtc gacattctcg ccatcaacaa
     4081 ggacgtacgt gatcggcgcg gccatgcacc gaacctagcc gacctgtcgc cgccgcaccc
     4141 acgcttgcca aggggcaagg acaccgaggt gagcgggcgg cataccggag tcgtcagagt
     4201 ctcccgacga gatgacgacc tccgcgtcct cccagccatc cggcagcgcg acctcgaccg
     4261 actccccgct gaggttcgcg acgaccaaca actcgtcgcc ctccagcgcc cgcgtgaacg
     4321 catagacgct cgggtggtcg gcgagcagca gctcgaaccg cccctcgacg accaccggca
     4381 gctcgcgccg cagccggatc agccgctggt agtgcgcgaa gaccgactcc gggtcaccca
     4441 cctgcgcggc cgcgttgatc tcccggtatg ccgggttgac cggcagccac ggcgtccccg
     4501 tcgtgaaccc cgcgtgctcg gacgcgtccc actgcatcgg cgtccgcgcg tggtcgcggc
     4561 tctggacgtt gaccgcggcg agcgccgccg cagggtccat gcccttgccc accgcctccg
     4621 ccgaccagtt gcgcgactcg acatcatcga gttgatcgag cgaggtgaag gggtagttga
     4681 ccatccccag ctcctccccc tggtagacgt agggggtgcc gcggtgcagg tgcagcaccg
     4741 tcgcgagcgc cttcgccgag acctcgcggt atgccgtgct gccggctccg tagcgcgaca
     4801 ccggtcgcgg ttggtcgtgg ttgcaccagt agagactgtt ccagccgaca tcggccaggc
     4861 cgtcctgcca gcgggccatg atcgacttca gccggaccca gtcgacgggg cggatgtcgt
     4921 agcgaccgcc catcccgtgg tcgacgccca tgtgctcgaa ctggaagacc atgtcgaact
     4981 cgccgcgcgc cgggtcggtg aaccgcaccg cgtcgtcgac ggtcgcgccc ggggcctcgc
     5041 cgacggtgat gagttgcttt ccgccccaag cattctcatg catctcggcg acgaactcgt
     5101 ggatccgcgg cccgttgaag aaccacccgg tgccgtcggc gttgaactgc ttggagacga
     5161 ggttgatgac gtcaaagcgg aagccgtcga cgccgcggtc gagccacca
//
LOCUS       DAORPW010000161         5174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4539_length_5174_cov_740.923813, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000161 DAORPW010000000
VERSION     DAORPW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4539_length_5174_cov_740.923813"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLB86_10730"
     CDS             <1..339
                     /locus_tag="PLB86_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRC65655.1"
                     /translation="PFFRDVEDHLARTNDHVESYDRLLSEILSAHLAKVTVQQNEDMR
                     RISAWVAMAAVPTMIAGIYGMNFEFMPELTAYVYIGDAKFYWGYFAIMGLMAVACGGL
                     FAFFRRHGWL"
     gene            complement(400..1254)
                     /locus_tag="PLB86_10735"
     CDS             complement(400..1254)
                     /locus_tag="PLB86_10735"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRC65656.1"
                     /translation="MSDLSYVQSVILGIVEGLTEFLPVSSTGHLTVTEKLLGLDVADP
                     AVTAYTAIIQLGAIVATFVYFWQDIVRLARAWFRGITRPDERALPDYALAWAVIVGSI
                     PVALVGFALRKVIAGPLRSLWVVAAALIIWSVVMWLAESRHAKLVREGAERGESTVTM
                     KDGLIIGLVQCFSLVPGVSRSGATISAGLFRGIDRVTATRLSFFLAIPALTAAGLFEA
                     ATTDLKGLGIGQMAVGVVVAFGVAYASIAWLLKFVAGHKLTAFVLYRVVLGALIVVAL
                     GAGWLTAQ"
     gene            complement(1261..2328)
                     /locus_tag="PLB86_10740"
     CDS             complement(1261..2328)
                     /locus_tag="PLB86_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRC65657.1"
                     /translation="MRLGLNLGYWGAGNDQSNVTLAEEADRLGYSVAWVAEAYGSDSP
                     TVLSWVGARTQRIHLGAAVMQIPARTPAMTAMTAATLDSLSGGRFRLGLGVSGPQVSE
                     GWHGVRFDAPLGRTREYVDIVRMALRRDVVSYEGRHFTLPLPDGPGKALKLGVRPVRD
                     GQIPIYLAAVGPKNLELTGEIADGWLAIFFSPEFAGDLIGSLETGARRAGKGADGSPP
                     LAGFDIVPTVPVVVTDDLDYAYAAVAGYAALYIGGMGSREKNFYNQLACRMGFEKEAK
                     LIQDLYLEGKARDAAAAVPREFLDATSLLGPRERIRDRLAAYQEAGVTTLSVAPYGPS
                     MEHRRAIVHTMAEVAQEAGLG"
     gene            2380..3321
                     /locus_tag="PLB86_10745"
     CDS             2380..3321
                     /locus_tag="PLB86_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRC65658.1"
                     /translation="MLSRHLGRSGLAVSRLGLGTLTWGVEVDEEGARDQLTAFVAAGG
                     TLVDTAHGYGAGAAETMLGTLLKARAVAREDLVLVTKAGIRQEGDRRIVDVSRRGLLR
                     ELDGSLARLGTDHVDVWLAHAWSDSVPLEETLGALEYAVSSGRARYVGVSNYSGWQTA
                     RAFSLLESARIPLVANEIEHSLLHRDGEHEVLPAAQALGFGVLAWAPQGRGILTGKYR
                     GGIPPQSRAASRVFPRFLERYLDERAVSISDAVVTAARGLDVPPSQVALAWARDTPGV
                     SAVLVGARTLTQLQTALGSESIDLPGPVRDALDDVSA"
     gene            complement(3328..3714)
                     /locus_tag="PLB86_10750"
     CDS             complement(3328..3714)
                     /locus_tag="PLB86_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65659.1"
                     /translation="MTADPRAALALLVSALERHLEVATTRRDPDDPGVIAAAEALAEA
                     FDDYDEVLFAATEVATPLAVYGDEDDFDDDDDEVVDPRAAVEGKVTVYAGLDDEDVDF
                     DDTDDEDDDDDEEDDDEDDEEEDERS"
     gene            complement(3785..5134)
                     /locus_tag="PLB86_10755"
     CDS             complement(3785..5134)
                     /locus_tag="PLB86_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRC65660.1"
                     /translation="MTSSQANGIRPEDEVARLCAELIRIPSVNAGDNQGPGERAAAEW
                     VMEQLHEVGLEPALVESSPGRASVMVRIEGADPSLPGLVVHGHTDVVPANAADWQVDP
                     FGAEERDGCIWGRGAVDMKDMDAMILASVRDLARTGAKPARTTTFVFFADEEAGGVYG
                     SQWVVANHPEWFEGASEAISEVGGFSVTVPTRHTGAPTRAYLLQTAEKGIAWLRLHAH
                     GRAGHGSVPNDSNAVVRLAQALARIDAHVWPREYVASVSGLLDGLSEITGIPYAAHDP
                     SVFPTDLLQTLGGAAGFVLGTLRDTAQPTMLSSGYKMNVIPQTATAAVDCRFLPGHEE
                     SLMATIRELAGEHVEVEVVHHDRALDAPFTGSLVQAMKQALLAEDPGAHVLPYCLSGG
                     TDNKALARLGITGYGFAPLRLPPDLDFVSMFHGIDERVPVDSLQFGARVLRRFLDLA"
BASE COUNT          754 a         1754 c         1868 g          798 t
ORIGIN      
        1 cccttcttcc gcgacgtcga ggaccacctg gcccgcacca acgaccacgt cgagtcctat
       61 gaccgactgc tctccgaaat cctcagcgcc cacttggcca aggtcaccgt ccagcagaac
      121 gaagacatgc gccggatctc ggcctgggtc gcgatggcgg ccgtgccgac gatgatcgcc
      181 ggcatctacg ggatgaactt cgagttcatg cccgagttga cggcctacgt ttacatcggc
      241 gacgccaagt tctactgggg ctatttcgcg atcatgggtc tcatggccgt cgcctgtggc
      301 gggctgttcg ccttcttccg caggcacggc tggctctgag cgcacgcgcc tcccgaccac
      361 caggtatgcc gcgtgcgcgg catacctcgc gggcttgcct cactgggcgg tgagccagcc
      421 cgctcccagc gcgacgacga tgagcgcgcc gagcacgacg cggtagagga cgaacgccgt
      481 caacttgtgc ccggcgacga acttgagcaa ccaggcgatc gaggcatagg cgaccccgaa
      541 ggccacaacc acgccgacgg ccatctgccc gatgcccagc cccttcaggt cagtggtggc
      601 cgcctcgaac aggcccgcag cggtcagcgc ggggatggcg aggaagaagg acagccgggt
      661 cgcggtcact cggtcgatcc cgcggaacag cccggccgag atcgtcgcgc cggagcggct
      721 gacgccgggg acgagcgaga agcactgcac gagcccgatg atcagcccgt ccttcatcgt
      781 caccgttgac tcgccacgct cggcgccctc gcgcaccaac ttggcgtggc gcgactcggc
      841 cagccacatg acgacgctcc agatgatgag tgccgcggcg acgacccaga ggctgcgcag
      901 cggcccggca atcaccttgc gcagggcgaa gcccacgagc gccaccggga tcgagccgac
      961 gatgaccgcc cacgccagcg cgtagtccgg cagcgcccgc tcgtcgggcc gggtgatccc
     1021 gcggaaccac gcgcgcgcca gccgcacgat gtcctgccag aagtagacga aggtcgccac
     1081 gatcgcgccg agctggatga tcgcggtgta tgccgtgacc gccgggtccg cgacgtccag
     1141 cccgagcagc ttctcggtca ccgtgaggtg cccggtcgag gacacgggca ggaactcggt
     1201 gagcccctcg acgatgccga ggatgaccga ctggacgtag ctgagatccg acacgaggga
     1261 tcagcctagc ccggcctcct gcgccacctc ggccatcgtg tggactatgg cgcgccggtg
     1321 ctccatcgac ggcccgtagg gcgcgaccga cagggtggtc accccggcct cctggtatgc
     1381 cgccaaccgg tcccggatcc gctcgcgcgg cccgagcagg ctggtggcgt cgaggaactc
     1441 ccgaggcacc gcggcggcgg catcgcgggc cttgccttcg aggtagaggt cctggatgag
     1501 cttggcttcc ttctcgaagc ccatccggca ggcgagttgg ttgtagaagt tcttctcccg
     1561 cgaccccatg cccccgatgt agagggcggc ataaccggcg accgcggcat acgcgtagtc
     1621 caggtcgtcc gtgacgacga cggggacggt gggcacgatg tcgaagccgg cgagcggcgg
     1681 gctgccgtcg gcgcccttgc ccgcgcggcg ggcgccggtc tcgagtgacc cgatgaggtc
     1741 accggcgaac tcgggcgaga agaagatcgc cagccagcca tcggcgatct cgccggtgag
     1801 ctcgaggttc ttggggccga ccgcggccag gtagatgggg atctgcccgt cgcggaccgg
     1861 tcgcacgccg agtttgaggg ccttgccggg cccgtccggc agcggcaggg tgaagtggcg
     1921 gccctcgtag ctaacgacgt cgcgtcgcag cgccatccgc acgatgtcga cgtattcgcg
     1981 ggtgcggccc agcggggcgt cgaagcgcac accgtgccag ccttcgctca cctgcggccc
     2041 gctgacgccc aggccgagcc ggaaccggcc gccggagagc gagtcgagcg tggccgcggt
     2101 catggcggtc atcgccgggg tgcgggccgg gatctgcatg acggccgcgc cgaggtggat
     2161 gcgctgggtg cgggcgccga cccaggacag cacggtcgga gagtccgagc cgtaggcctc
     2221 ggcgacccag gccaccgagt agcccagccg atccgcttcc tcggccagcg tgacgttgga
     2281 ttggtcgttg ccggcacccc agtagccgag gttcagtccg aggcgcatcg tgtgggtcac
     2341 cggaccaggc tagcggcgcg ggcggatagg ttgtgggtca tgctttcccg tcacctgggc
     2401 cgcagcggct tggctgtctc gcgcctgggc ctgggcaccc tcacgtgggg cgtcgaggtc
     2461 gatgaggagg gcgcccgcga tcagctcacg gccttcgtcg cggccggtgg caccctggtg
     2521 gacacggccc acgggtatgg cgcaggggcg gccgagacga tgctcggcac tctgctcaaa
     2581 gcccgcgccg tggcgcgcga ggatctcgtc ctggtcacca aggccggcat ccggcaggag
     2641 ggcgaccgac ggatcgtcga cgtgtcccga cgtgggttgt tgcgtgagtt ggacgggtca
     2701 ctggcgcggt tgggcaccga tcacgtcgac gtgtggctgg ctcacgcgtg gagcgacagc
     2761 gtcccgttgg aggagacgct cggagccctg gagtatgccg tgtcgtccgg tcgcgctcgc
     2821 tacgtcgggg tctccaacta ctcgggttgg cagaccgcac gcgccttctc gctgttggag
     2881 tcggctcgga tcccgttggt ggccaacgag atcgagcatt cgctgctgca ccgcgacgga
     2941 gagcacgagg tgttgccggc tgcgcaagcg ttgggcttcg gggtgttggc ctgggccccc
     3001 caggggcggg gcatcctgac cgggaagtat cggggcggca taccgccgca gtcccgggcc
     3061 gcatcccggg tcttcccacg gttcctggag cgctacctcg acgagcgggc ggtctcgatc
     3121 tccgatgcgg tcgtcacggc cgcacgtggg ttggacgtcc cgccgtcgca ggtcgcgctt
     3181 gcttgggctc gcgacacgcc gggggtgagc gccgtgctcg tgggtgctcg gacgctgact
     3241 cagttgcaga cggcgctggg cagtgagtcg atcgaccttc cgggcccggt ccgcgacgcg
     3301 ttggacgacg tctcggcctg agccggctca gctgcgctcg tcctcttcct cgtcgtcctc
     3361 gtcgtcgtct tcctcgtcgt cgtcgtcgtc ctcatcgtcg gtgtcgtcga agtcgacgtc
     3421 ctcgtcgtcg aggccggcat agacggtgac tttgccctcg accgcggcgc gcgggtcgac
     3481 cacctcgtca tcgtcgtcgt cgaagtcgtc ctcgtcgcca taaaccgcca gcggcgtagc
     3541 gacctccgtg gccgcgaata gcacctcgtc gtagtcgtcg aaggcttccg ccagggcctc
     3601 ggccgcggcg atgacgcccg ggtcgtcggg gtcgcggcgg gtcgtggcaa cctcgaggtg
     3661 gcgctccagc gcagaaacga gcagggcgag tgcggcacgc gggtcggcgg tcatgctacg
     3721 aaggtatcgg gtccacgtca cgaatgggcc accggtccgg cgaacgccct gtggacggtg
     3781 cctgtcaggc caggtcgagg aaccgccgca acaccctcgc gccgaactgc agggagtcga
     3841 ctggcacccg ctcgtcgatg ccgtggaaca tcgacacgaa gtcgaggtcc ggaggcagtc
     3901 gcagcggcgc gaagccgtat ccggtgatcc cgagccgggc cagcgccttg ttgtcggtgc
     3961 cgccggagag gcagtagggc aggacgtggg cgccggggtc ttcggccagc aaggcctgct
     4021 tcatcgcctg caccagtgag ccggtgaagg gcgcatcgag cgcacgatcg tggtggacga
     4081 cctcaacctc gacgtgctcc cccgcgagct cgcggatcgt cgccatgagc gactcctcgt
     4141 gcccgggcag gaaccggcag tcgaccgcgg cggtcgccgt ctgcgggatg acgttcatct
     4201 tgtagccgct ggagagcatc gtcggctggg cggtgtcgcg cagcgtcccg agcacgaatc
     4261 cggctgcgcc cccgagggtt tggagcaggt cggtggggaa gacggagggg tcgtgcgcgg
     4321 catacgggat gccggtgatc tccgagagac cgtcgaggag gccgctcacg gaggcgacgt
     4381 attcgcgggg ccagacgtgc gcgtcgatgc gcgcgagagc ctgcgcgagg cggacgaccg
     4441 cgttggagtc gttggggacc gagccgtggc cggcgcggcc gtgggcgtgc aggcgcagcc
     4501 aggcgatgcc cttctcggcc gtctgcagga ggtaggcgcg ggtgggtgct ccggtatgcc
     4561 gcgtcggcac cgtcacgctg aagccgccga cctcgctgat cgcctcactg gccccctcga
     4621 accactcggg gtggttggcg acgacccact ggctcccgta gacgccgccg gcctcctcat
     4681 cggcgaagaa gacgaaggtc gtcgtgcggg cgggcttggc gccggtgcgg gccaggtcgc
     4741 ggaccgacgc gagaatcatc gcgtccatgt ccttcatgtc gacggcgccg cggccccaga
     4801 tgcacccgtc gcgctcctcg gcgccgaacg gatcgacctg ccagtcggcc gcgttggcgg
     4861 ggacgacatc ggtgtgaccg tgcacgacga ggccgggcag gctcgggtcg gcgccctcga
     4921 tgcggaccat gacgctcgcg cggccggggc tgctctcgac gagggcgggc tccaggccca
     4981 cctcgtgcag ttgctccatg acccactcgg cggcggcccg ctcccccggc ccctggttgt
     5041 caccggcgtt gaccgacggg atgcggatga gctcggcaca cagccgggcc acctcgtcct
     5101 cgggacgtat gccgttcgcc tggctgctcg tcatggcccc aacctaacct ggcgagggct
     5161 ctgcaggcgg ggtg
//
LOCUS       DAORPW010000162         5154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4569_length_5154_cov_677.939400, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000162 DAORPW010000000
VERSION     DAORPW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4569_length_5154_cov_677.939400"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PLB86_10760"
     CDS             complement(<1..1034)
                     /locus_tag="PLB86_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRC65661.1"
                     /translation="MGAFSWKLKGDGHTLAPGDVVAPDERLAWGKTIGLGAQHVVAMF
                     GATFVFPLLMGLNAQLAIMMSGIATIIFLLMVSGKVPSYLGTSASFVGVAAAIRAQPG
                     ADSRTVTGAILIGGVVLALVGLLVHVAGSGIIHKVLPPVVTGAVVMLIGFNLAPVVAN
                     VYWPQDQWVALLVMTFAIVCAVALKGFLGRISIFLSLVFGFVLSWLFDRGFGQITSFD
                     AGAGKVTTHWRVNLDGVASSPWLGFPPATTTGADGKELVGLHAPTISFAAVLVVLPAI
                     IALIAENTGHVKAVAEMTGADLDHVMGRAIAADGVATAVASSVGGSPTTTYAENIGVM
                     AATRIYSTAA"
     assembly_gap    1130..1229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1230..1843)
                     /locus_tag="PLB86_10765"
     CDS             complement(<1230..1843)
                     /locus_tag="PLB86_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRC65662.1"
                     /translation="MSEPSGMPPRLTLQPLDESFERALVIVAHPDDIEYGTAAAVDKW
                     TKAGKTVTYLLATRGEAGIDSIAPEEAGPLRAQEERDSASEVGVDIVEFLDYDDGVVV
                     YGTDLRRDIARVIRHRRPQVIVTLTHDESFAGGMTNQADHRAVGLAAIDAARDAANRW
                     IFRELLEQEGLEPWGGITAVLSSAANGPTHYVDVTGHLEPAIASL"
     gene            1905..2315
                     /locus_tag="PLB86_10770"
     CDS             1905..2315
                     /locus_tag="PLB86_10770"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRC65663.1"
                     /translation="MSAPDLQAFLAREFPDIDRLGILVERVDERSITLRLPAESAMIR
                     PGGTISGPTMFSLADVTVWLLILAQIGPVALAVTTSVGMNFLRKPPPGDLVAEGELLK
                     LGRRLAIGHVRLHAEGSRDLVADASITYSIPPRG"
     gene            complement(2375..3145)
                     /locus_tag="PLB86_10775"
     CDS             complement(2375..3145)
                     /locus_tag="PLB86_10775"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HRC65664.1"
                     /translation="MTSAPIAPEIPGAIIALARAARRITVLTGAGISAESGVPTFRDA
                     QTGLWTQVDPEDLATPAAWARDKPYVNAWFLWRVHLISAATPHAGHRALADWARRPDT
                     DLRIVTQNIDDLHERAGSEVLAHLHGSLFAWRCDRCERPAPTPQAPAQPRERIEPDTC
                     PHCAFGEIRPGVVWFNEALPERELADAIRVCRDADLVVVVGTSGIVQPAASLPHLAGA
                     RGIPVIEIDPRPTEFSPFATHVWRATAGAALPALVAAL"
     gene            3300..4025
                     /locus_tag="PLB86_10780"
     CDS             3300..4025
                     /locus_tag="PLB86_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRC65665.1"
                     /translation="MDVNAYALFQARAESPPSATRRFFELFGGGPLPDWEGLDEATES
                     ISADAGQVIFDRGTEHPYVYVVLDGSVKLLAIDERANEHLVGLARAGELVASVGALAP
                     QGLARYVDEDLLGGNWSAESAMGTTDIRAVAIRACRLERVDFRVVHAKMREHGGTWAV
                     AVFNAALHYALVEEHRARQFLMLTAEDRYRHFLRRYPDLVGVLPQKDIGNYVGVTPVG
                     ISRIAARVRDEDRRRPIRTRAAQ"
     gene            4185..4889
                     /locus_tag="PLB86_10785"
     CDS             4185..4889
                     /locus_tag="PLB86_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65666.1"
                     /translation="MPLASDTGIDLPGDAAIPNAEPPPSSYTYRWLAHLADRPLPRRE
                     VFERAITQMTFEPGEAVFDSDQDLPYLFMVLRGGVKVADPTRENERLTGVAGVGDLVG
                     SLPSVLPAGMPDLLSAKVAESTWQPQPQWGQTNRSAIAIVDTVVERMDMRVLMSLMRR
                     HAAWAMVGYHAMALHALGQERRARDLLTLSPQERYLNFLVQAPELIGLLPQKDIAAYI
                     GVTPVGLSRIAARSRR"
     gene            complement(4943..>5154)
                     /locus_tag="PLB86_10790"
     CDS             complement(4943..>5154)
                     /locus_tag="PLB86_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65667.1"
                     /translation="LGADAGTALTNYVFRARDDERLGSATFVAAVVEALGGQPAVPPR
                     EEDRVDLAGMIGVAWRLRTALTATS"
BASE COUNT          797 a         1655 c         1829 g          773 t
ORIGIN      
        1 taggcggccg tcgaatagat ccgggtggcg gccatgacgc cgatgttctc ggcgtaggtc
       61 gtcgtcgggg agccgccgac cgaggaggcg accgcggtgg cgacaccgtc ggccgcgatc
      121 gcgcgaccca tgacgtggtc gaggtcggca ccggtcatct cggcgacggc cttgacgtgg
      181 ccggtgttct cggcgatgag ggcgatgatc gcgggaagga cgacgaggac cgcggcgaag
      241 gagatcgtcg gggcgtggag gccgacgagt tccttgccgt cggcgccggt ggtcgtcgcc
      301 ggcgggaagc ccagccacgg cgagctcgcg accccgtcga ggttgacccg ccagtgcgtc
      361 gtcaccttgc cggcgcccgc gtcgaagctg gtgatctggc cgaagccccg gtcgaacagc
      421 caggacagca cgaagccgaa gaccaggctg aggaagatcg agatccgccc gaggaagccc
      481 ttgagcgcga ccgcgcacac gatggcgaag gtcatgacca acagggcgac ccactggtcc
      541 tgcggccagt aaacgttggc gacgaccggc gcgaggttga agccgatgag catgacgacc
      601 gcgccggtga cgaccggagg cagcaccttg tggatgatgc ccgagccggc cacatggacg
      661 agcagaccga cgagcgcgag gacgacaccg ccgatgagga tggcgccggt gacggtgcgg
      721 gagtcggcgc ccggctgggc ccggatcgcg gcggcgaccc cgacgaacga cgccgacgtg
      781 ccgaggtagg acggaacctt gccgctcacc atgagcagga agatgatcgt cgcgatgccg
      841 ctcatcatga tcgccaactg agcgttgagg cccatgagca gcgggaacac gaaggtggcg
      901 ccgaacatcg cgaccacgtg ttgggcaccg agcccgatcg tcttgcccca ggcgaggcgt
      961 tcatcggggg cgacgacgtc gccgggggcg agggtgtgcc cgtcgccctt gagtttccaa
     1021 ctgaatgcgc ccatggatgc tgggctcctt cccgtcacga gatagtcgtt ccggggtttc
     1081 accctctcac gtccaggggt atgccgcgtg cctgccacgc ggcatacccn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcgaggcgat cgccggctcg aggtgcccgg
     1261 tgacgtccac gtagtgggtc ggcccgttgg cggcgctgga caggacggcg gtgatgccgc
     1321 cccacggctc gaggccctcc tgctcaagca actcgcggaa gatccagcgg ttggcggcgt
     1381 cccgcgcagc gtcgatggcg gccaggccga ccgcccggtg gtcggcctgg ttggtcatcc
     1441 ctccggcgaa cgactcatcg tgggtcagag tgacgatgac ctgcgggcgg cggtggcgga
     1501 tcacccgggc gatgtcgcga cgtagatcgg tgccgtagac gaccaccccg tcgtcgtagt
     1561 cgaggaactc cacgatatcg acgccgacct cgctggccga atcacgctcc tcctgggcgc
     1621 gcaacggacc ggcctcctcc ggggcaatgc tgtcgatgcc cgcctcgccg cgggtcgcca
     1681 gcaggtaggt caccgtcttg ccggccttgg tccacttgtc gacggcggcg gccgtgccgt
     1741 attcgatgtc gtccgggtgg gccacgatga cgagggcgcg ctcgaagctc tcgtcgagcg
     1801 gttgcagggt gaggcggggc ggcataccgg agggctcgct catggacgga cgatagcccg
     1861 cttcggcccc cgatggcggt gattaacttg aggtcgtgac cgcgatgagc gcacccgatc
     1921 tgcaggcgtt cctggcgcgc gagtttcccg acatcgatcg cctgggcatc cttgtcgagc
     1981 gggtggacga acggtccatc acgctgcgac tgccagcgga gtcggcgatg atccggcccg
     2041 gcgggacgat ctcgggcccg acgatgttct cgctcgccga tgtcaccgtg tggctgctca
     2101 tcctcgccca gatcgggccg gtcgccctcg cggtgacgac aagcgtcggg atgaacttcc
     2161 tgcgcaaacc cccgcccggt gatctcgtgg ccgaggggga actgctcaag ctcggtcggc
     2221 gcctggcgat cgggcacgtg cggttgcatg ccgagggcag ccgcgacctc gtggccgacg
     2281 cgtcgatcac gtactcgatc ccgccgcgcg gctgagcggg gttggctgag cggggttcgc
     2341 gagccgggtt cgcgagccgg gttcgcgagc cgggtcagag ggccgcgacg agggcgggta
     2401 acgccgcccc ggccgtcgca cgccacacgt gggtcgcgaa cgggctgaac tccgtggggc
     2461 gcgggtcgat ctcgatgacc ggtatgccgc gtgccccagc caggtgcgga aggctcgctg
     2521 ctggttggac gatgcccgag gtgccgacaa cgacgaccag gtcggcgtca cggcataccc
     2581 gaatggcgtc cgcgagctcg cgctcgggaa gcgcctcgtt gaaccacacc actcccgggc
     2641 ggatctcccc gaaggcgcag tgcgggcacg tgtccggctc gatccgctcg cggggctgcg
     2701 cgggggcctg cggcgtcggc gcggggcgct cgcagcggtc gcagcgccag gcgaagaggc
     2761 tcccgtgcag gtgcgcgagg acctcggaac cggcccgttc gtgcaggtca tcgatgtttt
     2821 gggtgacgat gcgcaggtcc gtgtccggcc ggcgggccca gtctgccagc gcacgatggc
     2881 cggcgtgcgg ggtggccgcc gagatgaggt gcacccgcca gaggaaccag gcgttgacgt
     2941 agggcttgtc gcgggcccag gcggccggag tggccagatc ctcggggtcg acctgggtcc
     3001 acaggccggt ctgtgcgtcg cggaaggtcg gcacaccgga ttcggcggaa atccccgcac
     3061 cggtgaggac ggtgatgcgc cgggccgctc gggcgagggc gatgatcgcg ccgggaatct
     3121 ctggcgcgat aggggccgag gtcataaatg ccatcatgcc agcgcccgaa atagcatcga
     3181 cgagagtgtc ggcaaaaccc cccgggagtc aaaccgtgct gcgataatga ccgcgaagcg
     3241 gaacgggacc gaaggcgcgc cgaccgcgtt gacgtgccgt gaactgccca ggagcgacca
     3301 tggacgtcaa cgcctacgcg ctgttccagg cccgggccga gtcgccgccg tcggccaccc
     3361 gacggttctt cgagttgttc ggtggtggcc cgctgcccga ttgggagggc ctggacgagg
     3421 ccactgagtc gatcagcgcc gacgcgggac aggtgatctt cgaccggggc acggagcacc
     3481 cctacgtcta cgtcgtgctg gatggctcgg tgaagttgct cgcgatcgat gagcgtgcga
     3541 acgagcacct cgtgggtctg gcccgagccg gcgagttggt cgccagcgtg ggggccctgg
     3601 caccccaagg cttggcacgg tacgtcgatg aggacctgct cggcgggaac tggagcgctg
     3661 agagcgctat gggaaccacc gatatccggg ccgtcgccat tcgcgcctgc cgccttgagc
     3721 gggtcgactt ccgggtggtc cacgcgaaga tgcgtgagca cggggggacg tgggccgttg
     3781 ccgtcttcaa cgcggcgctg cactacgcgc tcgtcgagga acaccgggcc cgccagttcc
     3841 tcatgctgac ggctgaggat cgctatcggc acttcttgcg gcgctacccc gacctggtcg
     3901 gggtgcttcc ccagaaggac atcggcaact atgtcggcgt cacaccggtc gggatcagtc
     3961 ggatcgccgc gcgcgtccgc gacgaggatc gtcgacggcc gatccgcacg cgtgccgctc
     4021 agtgacgtca caaaacacgc agagcacgcg aaggggacag aacgtcggca tcgacggctg
     4081 aatctttgtc ttgaggccac ccctggcatt cgtagaacgc gcaacgatcg gtatggtgca
     4141 tatggagggg agggactcgg ggagagaacc tgcaaggagc gcgtatgccg ttggcatcag
     4201 acaccggtat tgacctgccg ggcgatgcgg cgattcccaa cgccgaaccg cctccgtcca
     4261 gctacaccta tcggtggctc gcccacctcg ccgaccgccc cctgccccgc cgtgaggtgt
     4321 tcgaacgggc catcacccag atgacttttg agccgggcga ggcggtgttc gacagcgacc
     4381 aggacctgcc gtacctgttc atggtgctcc gagggggcgt caaggtcgcc gacccgacgc
     4441 gcgagaacga gcggctgacg ggggtggccg gtgtcggtga cctggtgggc agccttcctt
     4501 ccgtcctgcc cgccggaatg cccgacctgc tctccgccaa ggtcgccgag agcacgtggc
     4561 agccccagcc gcaatggggt cagaccaacc gctcggccat cgccatcgtc gacaccgtcg
     4621 tcgagcggat ggacatgcgg gtgttgatgt cgttgatgcg ccggcacgcg gcctgggcca
     4681 tggtcggata ccacgccatg gcgctgcatg ccttggggca ggagcgtcgg gcccgtgacc
     4741 tgctgaccct ctcgccgcag gagcgctatc tcaacttcct cgtccaggcg cccgagttga
     4801 tcggtctcct gccacagaaa gacattgccg cctacatcgg ggtcaccccg gtcgggttga
     4861 gccggatcgc cgcacgctcg cgccgctgag tcgctgagtc gctgagtcgc tgggtcgctg
     4921 agttgccgtc aggacgttgc cgtcaggacg ttgccgtcag ggcagttcgc agccgccagg
     4981 ccacgccgat catgccagcg aggtcgacgc ggtcctcctc gcgtggaggg acggccggct
     5041 ggccccccag cgcctcgacc accgcggcga cgaaggtcgc tgacccgagc cgctcatcgt
     5101 cgcgcgcccg gaagacgtag ttggtcaggg ccgtccccgc gtcggctccc aaca
//
LOCUS       DAORPW010000163         5153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4571_length_5153_cov_779.386622, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000163 DAORPW010000000
VERSION     DAORPW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4571_length_5153_cov_779.386622"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLB86_10795"
     CDS             <1..302
                     /locus_tag="PLB86_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HRC65668.1"
                     /translation="HVFIDYNQNARDHTIAAAYSVRGVPDGTVSTPIRWDEIADVEPR
                     SLTLATVPARYAALGDLHAGIDDTAYRLDALLEWAERDERAGADTPPEPDAGPDT"
     gene            520..1317
                     /locus_tag="PLB86_10800"
     CDS             520..1317
                     /locus_tag="PLB86_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRC65669.1"
                     /translation="MARRALAWGRRWWILTVVLVALAGLSGTLLVRDLRAPAPLTQAE
                     IDKAAAKVVDKALAEAARAPSAASTAYAQVAASLVIIETVGGGGDPAAQGLGTGVIVS
                     QEGAILTANHVVSGGGAITVTFADGTRAPARVQSAQPENDIAVLVADQLPQVVVPAVL
                     GGGVRVGDPVYAVGHPLGLTASLSSGVVSATGRTIEAEGLKLADLIQFDAAVNPGNSG
                     GPLLNSAGQVVGIVTALANPSKQPFFVGIGFAVPIATAGGAAGGPSI"
     gene            1326..2408
                     /locus_tag="PLB86_10805"
     CDS             1326..2408
                     /locus_tag="PLB86_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRC65670.1"
                     /translation="MSTPFAAGTSAAAPSPGPGAHPLEQMLYQVKRTIVGQDQLLERM
                     LVALLARGHLLVEGVPGLAKTMAIKTLAEAIGVDFKRIQFTPDLVPADLIGTRIYNQQ
                     TGDFQVSLGPVFANLVLADEINRAPAKVQSALLEVMQERQVTIGRETFSAPDPFLVMA
                     TQNPIESEGTYPLPEAQVDRFMLKVLVGYPSATEEYVVVERMIRAAERIDAVLDADGL
                     RGLQAAVDTVYVDPGVIQYAVALAGATRDPGVVGLPELARYLSFGASPRASINIVLAA
                     RALAYLRGRDYVIPMDARELAPDVLRHRMVLSYEALADDVSADDLLGPILAAVPLPET
                     AGGRHTSGVAEPIPANVDPAWYRRPE"
     gene            2387..3283
                     /locus_tag="PLB86_10810"
     CDS             2387..3283
                     /locus_tag="PLB86_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC65671.1"
                     /translation="MVPTTGVAAPERLLRRLEWTVVRRLDGRLQGDYRTIFRGTGVDF
                     RDLREYEPGDDLRHIDWNVTARTDVPFVREYTEDRELTAWLLLDRSASMAFGPTDRPK
                     HTVLLELATTLALVLARGGNRVGAVLYGDGVDRTVPPRQGRDQVLRIARELLADAPTA
                     GHPTDLGGLLRAALGMARQRSLIVIVSDFVTTPGWERPLGLLARRHDVVAIQLVDPRE
                     HDLPEAGLIALTDPETGEHVLVDTGDVEFRSRLWELAQARQRQLEAVAAHAGIALHPV
                     STDEDLVGALLRMARLRSKWRR"
     gene            3289..4275
                     /locus_tag="PLB86_10815"
     CDS             3289..4275
                     /locus_tag="PLB86_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC65672.1"
                     /translation="MTFASPWALLGLLLVPGLAAAYRIVRARRAARRAELAALGFAPV
                     LAGGRRRRGWVVPTALGLTLGLLVLAAARPQATVPDLRREGTVILAIDASTSMTATDV
                     APTRMAAAKAAAKAFVARQPSTIRIGVVSFGDSAVVLQAPTLVQAEAVAAIDRLTPQG
                     GTSLGQGIFASLKAIAGGKLAITEEQLAGDIESLDIGYFGSSAIVLLSDGEDTSRLDP
                     LALAELAGVAGVKINTVGLGDPAGTTVTVNGFTAATQLDEATLTAIADATGGRYRAAP
                     DEASLADVYGSIDLAMTSTPVHIEISALFAAAALLILVAAASTSILRTGRVI"
     gene            4276..>5153
                     /locus_tag="PLB86_10820"
     CDS             4276..>5153
                     /locus_tag="PLB86_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC65673.1"
                     /translation="MSFAWPYALLAVLLAPALLAAYLWSLRRRRRYAVRHPDLALVRA
                     ALPARSRWRPHLPVALLVLGVGLLGLAVARPQASVEVPIQRSTIILALDVSRSMCATD
                     VAPNRLSAARTAVQEFVETQPRDTRFGLVAFSGSAALVVPPTTDSAAVLTSLDGLSTG
                     RGTAIGSAILTSVDAIAAINSQVQPIGEVPLGPDTMQQPGVPAPVPSSIDPPPGATGG
                     FVSDIVVLLTDGANTRGVLPVAAAKLAAERRVRVYPIGFGTTNPTQMVCTAEQLGGDA
                     FGEFGPRSGGQVPGGQA"
BASE COUNT          702 a         1838 c         1803 g          810 t
ORIGIN      
        1 ggcatgtctt catcgactac aaccagaacg cgcgcgacca caccatcgcg gcggcctact
       61 cggtccgcgg cgtccccgac ggcaccgttt cgacgccgat ccggtgggac gagatcgccg
      121 acgtggagcc ccgatccctc acgctcgcaa cggttcccgc gaggtatgcc gccctcggtg
      181 accttcacgc ggggatcgac gacacggcat accggctgga tgccttgttg gaatgggccg
      241 agcgggacga gcgtgcggga gccgacacgc cacccgagcc ggacgccgga cccgacacct
      301 gatcgccgac gcctggacgc cgacgcctgg acgccgacgc ctgaccctgc ggcccgcccg
      361 acgccggaac gcgccaacct gtgaaatttc gatgaatccg caaccaccgg cgctcaggag
      421 gtaaggggcg gcttaccctc agtcatggcc ccgttgccac ctcggccggc acccccggct
      481 ccggctcccg aaacgccgca acccgcccgt gcgcgggtca tggcccggcg cgctctggcc
      541 tggggccgcc gctggtggat cctcacggtg gtcctcgtgg cactcgccgg cctctcgggc
      601 accctgctcg tgcgggacct acgcgccccc gcgccgctca cccaggccga gatcgacaag
      661 gccgccgcga aagtcgtcga caaggcgctg gccgaagccg cgcgggcccc gtcagccgcc
      721 agcacggcat acgcccaggt tgctgcgagc ctcgtgatca tcgaaaccgt tggcggggga
      781 ggcgatccgg ctgcgcaggg cctggggacg ggcgtcatcg tcagccagga gggcgcgatc
      841 ctcaccgcca accacgtcgt cagcggcggt ggcgcgatca cggtgacctt cgccgacggc
      901 acccgtgcac ccgctcgggt ccagagcgcg cagccggaga acgacatcgc ggtgctcgtg
      961 gccgaccaac tcccgcaggt cgtcgtgcca gcggtcctcg gtgggggcgt gcgcgtcggc
     1021 gacccggtgt atgccgtcgg ccatcccctc ggtctcaccg cgtcgctgtc gtccggtgtc
     1081 gtgtcagcga ccgggcgaac catcgaggcg gaagggctca aactggccga cctcatccag
     1141 ttcgacgcgg ccgtcaaccc gggcaattcc ggtgggccac tgctcaattc ggcaggtcag
     1201 gtcgtcggca tcgtcactgc gctcgccaac ccgtccaagc aacccttctt cgtcggcatc
     1261 ggtttcgccg tgccgatcgc gacagccggc ggcgccgccg gcggaccctc gatctaagga
     1321 gccagatgtc gactcccttt gccgccggca cctctgccgc cgccccctcg ccggggccag
     1381 gcgcccaccc gctcgagcag atgctctacc aggtgaagcg gaccatcgtc ggccaggacc
     1441 aactcctcga gcggatgctc gtcgcgctcc tggcccgtgg gcacctgctt gtcgagggcg
     1501 tccccggtct ggccaagacg atggcgatca agaccctcgc cgaggcgatc ggggtggact
     1561 tcaaacggat ccagttcacg cccgacctgg tccccgccga cctcatcggc acccggatct
     1621 acaaccagca gaccggcgac ttccaggtgt cgctcggccc tgtcttcgcc aacctcgtcc
     1681 tcgccgacga gatcaaccgg gcgccggcca aggtgcagag cgccctgctg gaggtcatgc
     1741 aggaacgtca ggtgacgatc ggccgcgaga ccttctccgc gccggacccg ttcctcgtca
     1801 tggccaccca gaaccccatc gagtccgagg gcacctatcc gctccccgag gcacaggtcg
     1861 accggttcat gctcaaggtg ctcgtcgggt atccctctgc cacagaggaa tacgtcgtcg
     1921 tcgagcggat gatccgcgcg gcggagcgga tcgacgcggt cctcgacgcg gacggacttc
     1981 gggggctgca ggccgccgtc gacacggtct acgtcgaccc cggagtgatc cagtatgccg
     2041 tcgcgctcgc gggtgcgacc cgtgacccag gcgtcgtggg gcttcccgaa ctggcccgct
     2101 atctgtcctt cggtgccagt cctcgcgcgt cgatcaacat cgttctcgcg gctcgggcgt
     2161 tggcctacct gcgtggccgt gattacgtca tcccgatgga tgcccgggag ctcgcgcccg
     2221 acgtcctgcg tcatcggatg gtgctcagct acgaggccct ggccgatgac gtcagcgccg
     2281 acgacctgct cggcccgatc ctcgcggcgg tgccgttacc cgagactgcg ggagggcggc
     2341 atacctcagg ggtggccgag ccgatccccg ccaacgtgga ccccgcatgg taccgacgac
     2401 cggagtagcc gcccccgagc gactgctgcg ccgtctggag tggacggtgg tgcggcggct
     2461 cgacgggcgg ttgcagggcg actaccggac catcttccgt ggcaccgggg tcgacttccg
     2521 cgatctgcgc gaatacgagc cgggcgacga cctgcgccac atcgactgga acgtcaccgc
     2581 gcgcaccgac gtgccgttcg tccgcgaata caccgaggat cgtgagctga ccgcctggct
     2641 gctgctcgac cggtcggcgt cgatggcctt cgggccgacc gatcggccca agcacacggt
     2701 gctgctcgag ctggcgacta ctctcgcact ggttctcgcg cgtggaggca accgggtggg
     2761 cgccgtcctc tatggcgacg gggtcgatcg cacggtgccg ccgcggcagg ggcgggatca
     2821 ggtgctgcgg atcgcccgcg agttgctcgc cgacgccccg actgcggggc acccgaccga
     2881 cctgggcggg ttgctgcggg cggcgttggg catggcccgg cagcgctccc tgatcgtcat
     2941 cgtgtcggac ttcgtgacga cgccggggtg ggagcggccg ttgggactgc tcgcccgtcg
     3001 gcatgacgtg gtggccatcc agttggtcga cccgcgggag catgacctgc cggaggccgg
     3061 gctcatcgcg ctcaccgacc ccgagaccgg ggagcatgtc ctcgtcgaca ccggcgacgt
     3121 tgagttccgt tctcggctat gggagttggc gcaggcgcgg cagcgccaac tggaggcggt
     3181 cgccgctcat gccgggatcg cgctgcaccc cgtgtcgacc gacgaggatc tcgtcggggc
     3241 gttgctgcgg atggcccgtc tgcgcagcaa gtggcggcgc tgatgccagt gaccttcgct
     3301 tcgccctggg cgctgctcgg gctccttctc gtgcccggtc tcgccgcggc ataccgaatc
     3361 gtgcgagccc gccgagcggc ccggcgcgcc gagctcgcgg cactcgggtt cgctccggtg
     3421 ttggccgggg gtcgtcgtcg acgaggttgg gtcgtcccga ccgcgctggg tctgacgctg
     3481 gggctgttgg tgctcgccgc cgcgcgtccg caggccaccg tgcccgacct gcgccgcgag
     3541 gggacggtga tcctcgcgat cgacgcctcg acgagcatga cggccaccga cgtggcgccg
     3601 acccggatgg cagcggccaa ggcggcggcg aaggccttcg ttgcgcggca gccgagcacg
     3661 atccggatcg gggtcgtgtc cttcggggac agcgcggtgg tcctgcaggc tccgacgctc
     3721 gtgcaggccg aggccgtggc ggccatcgat cggttgacgc cccaaggggg cacgtcgttg
     3781 gggcagggaa tcttcgcgtc gctcaaggca attgctggag ggaagctcgc gatcaccgaa
     3841 gagcagttgg cgggggacat cgagagtctc gacatcgggt acttcgggtc atcggccatc
     3901 gtcctgctgt ccgacggtga ggacacctcg cgcctggacc ccctggcatt ggccgagctc
     3961 gccggtgtcg ccggtgtcaa gatcaacacc gtcgggctcg gcgacccggc cgggacgacg
     4021 gtgaccgtca acggcttcac ggcggcgacg cagttggacg aggcgacgct gacggcgatc
     4081 gccgacgcga cgggtgggcg ctatcgggcg gcacccgacg aggcgtccct cgccgatgtc
     4141 tacggctcga tcgacctcgc gatgaccagc acccccgtgc acatcgagat ctcggcgctg
     4201 ttcgcggcgg ccgccctgct catcctggtc gccgccgcca gcacgtcgat cctgcggacc
     4261 gggcgggtga tctgagtgtc gttcgcgtgg ccctacgccc tgctcgcggt gctgctcgct
     4321 ccggcgctgc tcgcggcata cctctggtcg ttgcgacgga ggcgcaggta tgccgtccgg
     4381 catccggatc tcgcgctcgt gcgtgccgcg ctgcccgcgc gttcccgctg gcgtccgcat
     4441 ctgccggttg cgctgctcgt gctgggcgtc gggctgttgg ggctggcagt cgcccggccg
     4501 caggcctcgg tcgaggtccc gatccagcgc tcgacgatca tcctcgcgct ggacgtgtcg
     4561 cgctcgatgt gtgccaccga cgtcgcgccc aaccggcttt cggccgcgcg gacggccgtc
     4621 caggagttcg tcgagaccca gccgagggac actcggttcg ggctggtcgc gttctcggga
     4681 tcggctgcac tcgtcgtgcc gccgacgacc gactcggcgg cggtgctgac gtcgctcgac
     4741 gggctgagca ccgggcgcgg gacggcgatc gggtcggcca tcctcacgtc ggtcgatgcg
     4801 atcgccgcga tcaactccca ggtgcaaccg atcggcgagg tgccgctcgg gcccgacacc
     4861 atgcagcagc cgggcgtccc ggcgccggtg ccgagttcga tcgacccgcc gcctggagcg
     4921 actggcggct tcgtgtcgga catcgtcgtg ctgctcaccg acggcgcgaa cacgcgtggg
     4981 gtgctgccgg tggcggcggc caaactcgcg gccgagcgac gcgtcagggt ctatcccatc
     5041 ggtttcggca cgacgaatcc gacccagatg gtgtgcaccg ccgagcagtt gggcggggac
     5101 gctttcggtg agtttgggcc gcgatccggc gggcaggtcc cgggtggaca ggc
//
LOCUS       DAORPW010000164         5084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4685_length_5084_cov_875.689004, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000164 DAORPW010000000
VERSION     DAORPW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4685_length_5084_cov_875.689004"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="eno"
                     /locus_tag="PLB86_10825"
     CDS             complement(<1..977)
                     /gene="eno"
                     /locus_tag="PLB86_10825"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRC65674.1"
                     /translation="MASIEAVGAREILDSRGNPTVEVEVLLDDGTVARAAVPSGASTG
                     AFEAYEKRDGDKGRYLGKGVEKAVDAVLDEINPKIMGFDASEQRLIDAEMLAMDGSKN
                     KENLGANAILGVSLAVARAAADSANLPLFRYVGGPNAHVLPVPMMNILNGGSHADTNV
                     DIQEFMIAPIGAPTFKEALRWGAEVYHTLKKVLHDRGLSTGLGDEGGFAPNLESNRAA
                     LDVIVEAIEKAGYVPGKDIALALDVAATEFYKDGAYQFEGAAKTSAEMTAYYGELVAA
                     YPLVSIEDPMSEEDWDGWKAITVALGDKVQIVGDDLFVTNPERLQRGIDT"
     gene            complement(1074..1736)
                     /locus_tag="PLB86_10830"
     CDS             complement(1074..1736)
                     /locus_tag="PLB86_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HRC65675.1"
                     /translation="MTGGPGPSVPSGAALLDLVAVMDRLRSPGGCPWDAEQTHASLVP
                     YLVEETYEAVEAIESGERDHMAEELGDVLLQVVFHARVAQEDEVAPFDIDDVAAGIAA
                     KLRRRHPHVFGDVVADTPEAVAANWSVIKAAEKAAKGSGTAPPGPLHGIPAGMPPLAK
                     AAAMVARLDRAGRGDLVDAAAAGSDPGSRLLAVVADVVRRRDDPDAVLRATLRSLSDA
                     AG"
     gene            complement(1747..2277)
                     /locus_tag="PLB86_10835"
     CDS             complement(1747..2277)
                     /locus_tag="PLB86_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65676.1"
                     /translation="MSPRTPRRLGTLPAALIGLLVATLIAGCGAAGRPGTALVVDGQS
                     YTPSQIVETTAQINEALKAGGEAATEAQIVNLLMISPFVLAEATSRGLWGPDNQYNSF
                     MTAIHNPTPVTVAVVQASAAYASLDEEGKAALLKDIKAASIAVDPRYGSVDYSTGFLG
                     MPTPDWIVRTTTAPEK"
     gene            complement(2274..>5084)
                     /gene="mfd"
                     /locus_tag="PLB86_10840"
     CDS             complement(2274..>5084)
                     /gene="mfd"
                     /locus_tag="PLB86_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRC65677.1"
                     /translation="AVEGMESLQPVLVEAMQTLPDTLGAGSLIVLADPERIRTRAHDL
                     VATSEEFLEASWSNAAAGNAVPIDLRGVLDEGSYRTLAGLRQDAITSGMPWWSLTAFA
                     SDAELTDFLDDDTSEQRLDLGLREPDAYRGDTEAAVADLGRWIHDGWTVLTVTEGPGL
                     ARRVHEVLAERGIPSRLVGHEAESAPGVVTVTTGALGRGFVDETGQFALLTETDLTGS
                     AGSATTTKDMRRMPSRRRAQVDPLSLRPGDYVVHEQHGVGRFVEMTARTVGGATREYL
                     VLEYAASKRGQPGDRLYVPTDQLDQVTKYVGGEAPTLHRLGGSDWAQTKARARRHVKE
                     IAADLIRLYSVRMSTVGHAFAPDTPWQRELEDAFAYVETPDQISSIDEVKADMEREVP
                     MDRLICGDVGYGKTEIAVRAAFKAVQDGKQVAILVPTTLLVQQHLQTFTERYAQFPVT
                     VKALSRFQSTTEAGRVVDGLADGSVDVVIGTHRLLTDKIRFKDLGLVVVDEEQRFGVE
                     HKEKLKTFRTAVDVLAMSATPIPRTLEMAVTGIREMSTLATPPEERHPVLTFVGSYDE
                     KQITAALRRELLREGQVFYVHNKVQSIEKAAARLRELVPEARIATAHGKMTEGRLEQV
                     VLDFWEKRFDVLVCTTIVETGLDISNANTLIVERADLLGLSQLHQLRGRVGRGRERAY
                     AYFLFPPEKPLTETAHDRLATIASHTDLGSGMQVAMKDLEIRGAGNLLGGEQSGHIEG
                     VGFDLYVRLVGEAVAAYKGEAQAPPAEVKIELPVDAHLPHDYVPGERLRLEAYQKLAS
                     VQDEAGVAAIEEELRDRYGMPPEPVTNLIEVARLRTVARLAGLSDISAQGAFVRFGPV
                     PAEAMPDSRRLRLQRLYPGSIVKDQLGHLLIPRPMTARVGGKPVRDTAVLRWAADVIR
                     AVLVDNVGDAARVATNGKDLRP"
BASE COUNT          713 a         1708 c         1845 g          818 t
ORIGIN      
        1 gtgtcgatgc cgcgctgcag ccgctcgggg ttggtgacga acaggtcgtc gccgacgatc
       61 tggaccttgt cgccgagggc gacggtgatg gccttccagc cgtcccagtc ctcttcgctc
      121 atcgggtctt cgatcgacac cagcgggtag gccgcgacga gctcgccgta gtaggcggtc
      181 atctcggcgg acgtcttggc ggcaccctcg aactggtagg cgccgtcctt gtagaactcg
      241 gtggccgcca cgtcgagggc cagcgcgatg tccttgcccg gaacgtagcc ggccttctcg
      301 attgcctcga cgatgacgtc gagagcagcg cggttgctct cgaggttcgg ggcgaagcca
      361 ccctcgtcac ccaggccggt ggacaggcca cggtcgtgca ggaccttctt gagggtgtgg
      421 tagacctcgg cgccccagcg cagggcctcc ttgaaggttg gcgcaccgat cggagcgatc
      481 atgaactcct ggatgtccac gttggtgtcg gcgtgcgagc caccgttgag gatgttcatc
      541 atcgggacgg gcaggacgtg cgcgttgggg ccgccgacgt agcggaacaa cgggaggttg
      601 gccgagtcgg ctgccgcacg ggcgaccgcg agcgagacgc cgaggatggc gttggcgccg
      661 aggttctcct tgttcttgga gccgtccatg gcgagcatct cggcgtcgat gagccgctgc
      721 tcgctggcgt cgaagcccat gatcttgggg ttgatctcgt cgaggaccgc gtcgacggcc
      781 ttctcgacac ccttgccgag gtaacggccc ttgtcgccgt cacgcttctc gtaggcctcg
      841 aacgcaccag tcgacgcacc cgacgggacg gcggcgcggg ccacagttcc gtcgtcgagg
      901 agcacctcga cctcgaccgt ggggttgccg cgcgagtcga ggatctcgcg agcgcctact
      961 gcttcgatgc tggccacttc ggtgcctccg aacgtgttgg gatggggata aaggtcggcc
     1021 cggtgttgat ggcgcgcggc actgcgcagc cgcacccagc cgtcgcgccc accctatccc
     1081 gcagcgtcgc tcagggacct cagcgtggcg cgaaggacag cgtcaggatc gtcacgtcgg
     1141 cgcaccacgt cggccaccac ggccagcagt cgggagcccg ggtcggaccc ggcggccgcc
     1201 gcatccacga ggtcaccgcg cccggcccgg tcgaggcggg ccaccatggc cgcggccttg
     1261 gcgaggggcg gcatacccgc gggtatgccg tgcagcgggc ctggtggcgc ggtgccggac
     1321 cccttggcag ccttctcggc cgccttgatc accgaccagt tggcggcgac cgcctcgggg
     1381 gtgtcagcga cgacgtcccc gaagacgtgc gggtggcggc ggcgcaactt ggcggcgatc
     1441 ccggcggcga catcgtcgat gtcgaacggg gcgacctcgt cctcctgcgc aacgcgcgcg
     1501 tggaagacga cctgcagcag gacgtcgccg agttcctcgg ccatgtggtc gcgttcaccc
     1561 gactcgatcg cctcgaccgc ctcgtaggtc tcctcgacga ggtagggcac gagcgaggcg
     1621 tgggtctgct cggcgtccca cgggcagccg ccgggcgagc gcaaccggtc catgaccgcg
     1681 accaggtcca gcagcgccgc gccggatgga acgctcgggc ctgggccgcc cgtcacccgc
     1741 ggtcggtcac ttctcggggg cggtggtggt ccggacgatc cagtcgggcg tgggcatccc
     1801 caggaagccg gtcgagtagt ccaccgatcc gtagcgcggg tcgaccgcga tagaggcggc
     1861 cttgatgtcc ttgaggaggg cggccttgcc ctcctcgtcg agcgaggcgt aggccgcgct
     1921 ggcctgcacc acggcgaccg tcacgggggt cgggttgtgg atcgcagtca tgaaggagtt
     1981 gtactggttg tccggccccc agagaccgcg gctcgtcgcc tcggcgagga cgaacggcga
     2041 gatcatgagg aggttgacga tctgcgcctc ggtggcggcc tcgccgccgg ccttgagcgc
     2101 ctcgttgatc tgcgcggtcg tctcgacgat ctggctcggc gtgtagctct ggccgtcgac
     2161 gacgagagcc gtgcccgggc gtccggcggc accgcacccg gcgatcaggg tggcgacgag
     2221 cagtccgatg agggctgccg ggagcgttcc gaggcgacgc ggggtccgcg ggctcatggg
     2281 cgcaggtcct ttccgttcgt ggcgacgcgt gcggcgtccc cgacattgtc caccaagacc
     2341 gcacggatca cgtcggcggc ccagcgcagc accgcagtgt cgcgcaccgg cttgcccccg
     2401 acgcgggcgg tcatcgggcg agggatgagc agatggccga gttggtcctt gacgatcgac
     2461 cccggataga gccgctgtag gcgcagccgg cgactgtcgg gcatggcttc ggccggcacc
     2521 ggtccgaacc gcacgaaggc cccctgcgcc gagatgtccg acagcccggc caggcgggcg
     2581 acggtacgca gccgcgccac ctcgatgagg ttggtgaccg gctcgggcgg cataccgtag
     2641 cggtcgcgca actcctcctc gatcgcggcg acgcccgcct cgtcctgcac ggacgcgagc
     2701 ttctggtagg cctccaggcg caaccgctcg ccggggacgt agtcgtgcgg gaggtgcgcg
     2761 tcgaccggca actcgatctt cacctcggcg ggtggggcct gcgcctcccc cttgtatgcc
     2821 gcgaccgcct caccgacgag gcgcacgtag aggtcgaagc cgacgccctc gatgtgcccc
     2881 gactgctcgc cgccgaggag gttgcccgcg ccgcggatct cgaggtcctt catcgccacc
     2941 tgcatgcctg agcccaggtc ggtgtggctg gcgatcgttg cgagccggtc gtgcgcggtc
     3001 tcggtgagcg gcttctcagg cgggaagagg aagtaggcgt aggcgcgctc ccggccgcga
     3061 ccgacccgcc cgcgcaactg gtgcaactgg ctcaggccga gcaggtcggc gcgctcgacg
     3121 atgagggtgt tggcgttgga gatgtccagg ccggtctcga cgatcgtcgt gcacacgagc
     3181 acgtcgaacc gcttctccca gaagtcgagg acgacctgct ccaaccggcc ctcggtcatc
     3241 ttgccgtggg ccgtggcgat gcgggcctcg gggacaagct cgcgcagccg ggcggcggcc
     3301 ttctcgatcg actgcacctt gttgtggacg tagaacacct ggccctcgcg cagcagctcg
     3361 cggcgcaacg ccgcggtgat ctgcttctcg tcgtaggagc cgacgaaggt gaggaccggg
     3421 tgccgctcct cgggaggcgt tgcgagagtt gacatttcgc gtatgccggt cacggccatc
     3481 tcgagggtgc gcgggatggg ggtggctgac atcgccagca cgtcgacggc cgtccggaag
     3541 gtcttgagct tctccttgtg ctccacgccg aagcgctgct cctcatcgac gacgaccagg
     3601 ccgaggtcct tgaagcggat cttgtcggtg agcaaccggt gggtgccgat gacgacgtcg
     3661 accgacccgt cggccagccc gtcgacgacc cgcccggcct cggtcgtcga ctggaagcgg
     3721 ctgagcgcct tgaccgtcac ggggaactgc gcataccgct cggtgaaggt ctgcaggtgc
     3781 tgttggacca gcagcgtggt cggcacgagg atggcgacct gcttgccgtc ctgcacggcc
     3841 ttgaaggcag cccggacggc gatctcggtc ttgccgtaac cgacgtcgcc acagatgagc
     3901 cggtccatgg ggacctcgcg ctccatgtca gccttgacct cgtcgatgct ggagatctga
     3961 tcgggggtct cgacgtaggc gaaggcgtcc tcgagctcac gctgccaggg cgtgtccggg
     4021 gcgaaggcat gcccgaccgt cgacatccgc acggagtaga gccggatgag gtcggccgcg
     4081 atctccttga cgtggcgccg ggctcgggcc ttggtctggg cccagtccga gccaccgagt
     4141 cggtgcagcg tcggggcctc gccgccgacg tacttcgtca cctggtcgag ttggtcggtg
     4201 ggcacgtaga ggcggtcgcc gggctgcccc cgcttgctcg cggcatactc cagcacgagg
     4261 tattcgcggg tggcgccgcc gacggtgcga gcggtcatct cgacgaaccg acccaccccg
     4321 tgctgctcgt ggacgacgta gtcgccgggc cgcagtgaga gcgggtcgac ctgcgcacgc
     4381 cgccgggacg gcatccggcg catgtccttg gtggtcgtcg ccgaccccgc cgacccggtg
     4441 aggtcggtct cggtgagcag cgcgaactgg cctgtctcgt cgacgaatcc gcgccccagg
     4501 gcgcccgtcg tcaccgtcac gacccctggc gccgactccg cctcgtgccc gacaagccgc
     4561 gacggtatgc cgcgctcggc caggacctcg tgcacccgtc gggccagacc cggcccttcg
     4621 gtgactgtga ggacggtcca gccgtcgtgg atccagcgac cgaggtcggc caccgcagct
     4681 tcggtgtcgc cgcgataggc gtccggctcg cgcagcccga ggtcgaggcg ctgctcggag
     4741 gtgtcgtcgt cgaggaagtc ggtcagctcg gcatcagaag cgaaggcggt gagggaccac
     4801 cacggcatac cactggtgat ggcgtcctgg cgcagtcccg cgagcgtgcg gtaggagccc
     4861 tcgtcgagca ccccccgcag gtcgatcggg acggcgttcc cggccgcggc gttggaccaa
     4921 ctggcttcga ggaactcttc gctcgtggcc acgaggtcgt gggcgcgggt gcggatgcgt
     4981 tcggggtcgg ccaggacgat gagcgagccg gcgccgagcg tgtcggggag ggtctgcatg
     5041 gcctcgacga ggaccggttg cagcgactcc atgccctcga cggc
//
LOCUS       DAORPW010000165         5038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4760_length_5038_cov_763.298615, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000165 DAORPW010000000
VERSION     DAORPW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4760_length_5038_cov_763.298615"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLB86_10845"
     CDS             complement(<1..188)
                     /locus_tag="PLB86_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65678.1"
                     /translation="MSLTRIRLLVWKEFLQLRRDPLLLRLLLLMPIIQLVGFGYVVSV
                     DVRNLPTAVVDLDQTVVSR"
     gene            complement(185..1324)
                     /locus_tag="PLB86_10850"
     CDS             complement(185..1324)
                     /locus_tag="PLB86_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC65679.1"
                     /translation="MSGAGVRIGAVVRKEFIHVGRDPRLLAVVLIMPIIQLLLFAYAI
                     SFDVRHLPTVVVDLDHTPDSAAYLRAYEASGLFDVVGHGADPTAVDTAFDRNEAQVAV
                     IVPAGFAASLARGEQAQVGVLVDGSEPNSARVAQTYSMALNQLYGRALAVEWASTQGV
                     DLGQIGLVEPRLRTWYNPDRASSIFLVPGLMVVIIMIVTAQQTAVTLVREREQGTAEQ
                     LMVSPLRRIELMIGKLTPWVIMAFLDIVVIAVLGVVLFGVPMRGDPVALAVGATLFVA
                     SCLGIGLIVSAVAPSLDTANMAAFLVAFLPAFLLSGFAFALDQIPPVLEWISRLFPAR
                     YMVTISRSVLLKGAGFPEVWPQVAALAAYALISLLIAVRLHGRRA"
     gene            complement(1321..2271)
                     /locus_tag="PLB86_10855"
     CDS             complement(1321..2271)
                     /locus_tag="PLB86_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65680.1"
                     /translation="MAITGASPAAPAVSTQGLTRRFGDVVAVDGVDLVVQRGAVFGLL
                     GPDGAGKSTLIRMLATVLLPDAGEALVLGHSTASEASAITGRIGYMSQRFSMYPDLTV
                     AENLDFFATVRGVPKQVRRERSAALLDSMGLAEFGGRQARHLSGGMKQKLMLATTLMH
                     EPELLLLDEPTTGVDPVSRREFWRVLAGLHRGGTTVVVATPYMDEAERCSAIAFLQDG
                     RIQQQGTPEEITALVPGTLVEVSGADPRAALAALADLPGVGSPHVFGEVVRVLWEGSL
                     ASVEGAITTHLRAAGFPTVRVTPTRVDMETAFAVLAEAGR"
     gene            complement(2273..3058)
                     /locus_tag="PLB86_10860"
     CDS             complement(2273..3058)
                     /locus_tag="PLB86_10860"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC65681.1"
                     /translation="MAGHGRKHPPIPVIVVLVLALAGGGWWWWRTANQPEASASTALN
                     GQVEARQYDISSAIAGRVLTVPVAEGDQVTKGQPIVTLDTAALDLQLKQAQQGVIAAQ
                     AAVTNAKDSGTKADVTAAEARVEQANAAVSLAQVQLGYASVSAPRDGRVVTVVTNVGQ
                     NAAPGRILASIVDLHDLFVRVYVPETRIGTVSVGQAVSVSSDSGGAAIAGTVSFVSAQ
                     AEFTPNTVQTAEQRAKLVYEVRVTISDSSGTLKPGMPVDVTLR"
     gene            complement(3100..4554)
                     /locus_tag="PLB86_10865"
     CDS             complement(3100..4554)
                     /locus_tag="PLB86_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRC65682.1"
                     /translation="MSGHLPRPVCRPAGHSSTLATRGATPTTATRLAAIAALAVFATA
                     LGACSRDTATLHLVGTVQESLTVVAMPAIGMPTVDLDAGFAATQTTAQSQAPAQQANV
                     RTVSPTVIITGLGGVWRLATVTVREGSQVTAGQPIATVEDGILAAGVRAAEATAATAA
                     AQVPVLADRIAEVDDQRATLTDKHGQLTTALATIASSRATLTTNRTQLNANQTALQGN
                     RAGLAQTLEDLTATQTTLRAILAGLPTDPSVPLPPGTPTRAELTAQLAALATGIATAT
                     AGLAKLDAGLTEMRSGLKQIDSGLAQLATKASEVRTGLSTVEEGRAALADARVQLVDA
                     QAISRVMADTAAVAVDLAREQQARTTVTAPVTGVVVKAASAGDLLPPGAVLATIRPTG
                     PATIQVWLAPDQLATICRDDPAQVRGDWMPDGVAVPGTVTLIGDRADYPPSSNVTDEV
                     HLTRAVPVDVTTQNPLPPGVPVEVTLRGCHPHTK"
     gene            complement(4551..>5038)
                     /locus_tag="PLB86_10870"
     CDS             complement(4551..>5038)
                     /locus_tag="PLB86_10870"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HRC65683.1"
                     /translation="YSYFPSKGELFADFLDGELGHLRVDVGAPAPITSTAQLRATLLA
                     VATALVRRFMRDDTINLLRVVLGDAYRVPEVRDAVRDVIPNQLFGLVEAILGAAAAEG
                     VVEPPQPGLTARMFIGPVVSFVILDGLLVDGPPRLPDAHALGYVVDAFLATLAPAGDR
                     P"
BASE COUNT          795 a         1631 c         1888 g          724 t
ORIGIN      
        1 cgcgagacga ccgtctggtc gaggtcgacg acggccgtcg gcaggttgcg cacgtcaacg
       61 gacacgacgt acccgaagcc gacgagttgg atgatcggca tgagcagcag cagccgcaac
      121 aacagcgggt cacgccgcag ctggaggaac tccttccaca ccagcaggcg gatgcgggtc
      181 aggctcatgc gcgcctcccg tgcagccgga ccgcgatgag caggctgatg agggcgtatg
      241 ccgcgagcgc cgccacctgc ggccacacct cggggaagcc ggcgcccttg agcagcaccg
      301 agcgcgagat cgtcaccatg tagcgggccg ggaagagccg actgatccac tcaaggaccg
      361 gcgggatctg atcgagcgca aaggcgaacc cggacaacag gaacgccggc aggaacgcga
      421 cgaggaaggc cgccatgttg gccgtgtcga ggctcggcgc gacggccgag acgatgaggc
      481 cgatgcccag gcaggaggcc acaaagaggg tcgcaccgac cgccaacgcc acggggtcgc
      541 cccgcatcgg caccccgaag aggaccaccc cgagcacggc gataacgacg atgtcgagga
      601 acgccatgat gacccacgga gtcagcttgc cgatcatgag ctcgatccgc cgcagggggc
      661 tgaccatgag ttgctcggcg gtgccctgct cccgctcgcg caccagcgtc acagcggtct
      721 gctgggcggt gacgatcatg atgatgacca ccatgagccc gggcacgagg aagatcgacg
      781 atgcccgatc agggttgtac cacgtgcgca atctcggctc caccagcccg atttggccga
      841 ggtcgacccc ttgggtcgag gcccattcga cagcgagcgc gcgcccatag agctggttga
      901 gagccatgct gtaggtctgc gcgacgcgcg ccgagttggg ctcgctgccg tcgacgagga
      961 ctcccacctg ggcctgctca ccgcgcgcga gagacgcagc gaacccggcg gggacgatga
     1021 cagccacctg ggcctcgttg cggtcgaacg ccgtgtcgac cgcagtcggg tcggcgccgt
     1081 gcccgaccac gtcgaacagc ccggacgcct catacgcccg caggtatgcc gcgctgtcgg
     1141 gcgtgtggtc caggtcgacg acgacggtgg gcaggtgtcg gacatcgaag ctgatcgcgt
     1201 aggcgaagag cagcagttgg atgatcggca tgatgaggac gaccgcgagc agccgcgggt
     1261 cgcgcccgac gtggatgaac tccttgcgca ccacggcgcc gatccggact cctgcgccgc
     1321 tcatcggccg gcctcggcga ggaccgcgaa ggcggtctcc atgtcgacgc gcgtgggagt
     1381 cacccggacc gtggggaatc cggccgcccg caagtgggtc gtgatggccc cctcgacgct
     1441 cgcaagcgag ccctcccaga gcactcggac gacctcgccg aagacgtggg gtgagccgac
     1501 tcccggcagg tcggcgagcg cggcgagagc ggctcgcggg tcggctcccg acacctcgac
     1561 cagggtgccg ggaacgaggg cggtgatctc ctccggcgtg ccctgttgct ggatccgtcc
     1621 gtcctggagg aaggcgatcg ccgagcaacg ctcagcctcg tccatatagg gcgtggccac
     1681 gacgacggtg gtgccgcccc ggtgcaaccc ggccagcacc cgccagaact cccggcgcga
     1741 caccgggtcg acaccagtgg tgggttcgtc gagcaggagc aactcgggct cgtgcatgag
     1801 ggtggtggcg agcatgagct tctgcttcat tccccccgac agatgccgcg cctggcgacc
     1861 gccgaactcg gccagcccca tgctgtccag caaggccgcg gatcgctcgc gccggacctg
     1921 cttcgggacg cctcggacgg tcgcgaagaa gtcaaggttc tcggcgacgg tgaggtcggg
     1981 atacatcgaa aaccgttgcg acatatagcc gatccggccg gtgatggccg aggcctcaga
     2041 ggcggtcgaa tgccccagga ccaaggcctc ccccgcgtcc ggcagcagca cggtggccag
     2101 catccggatg agcgtcgact tgccggcccc gtccggaccg agcagaccaa agaccgcgcc
     2161 gcgctgcacg acgaggtcga ccccgtcgac cgccacgacg tcgccgaagc gtcgggtgag
     2221 tccctgcgtc gacaccgccg gtgcggcagg gctggctcca gtgatggcca tgtcagcgca
     2281 aggtgacgtc gaccggcata cccggtttga gggtccccga ggagtcagag atggtgaccc
     2341 gcacctcgta aacgagcttc gcccgctgct cagccgtctg cacggtgttg ggggtgaact
     2401 ccgcctgcgc cgagacgaag gagacggtgc ccgcgatggc ggcaccgccg gagtcggagg
     2461 acaccgacac cgcctgaccg accgacaccg tgccgatgcg ggtctcggga acatagaccc
     2521 ggacgaacag gtcgtgcaga tcgacgatcg aggcgaggat ccgcccaggg gcggcgttct
     2581 ggccgacgtt ggtgacgacc gtgacgaccc ggccgtcgcg aggtgcgctc acggaggcgt
     2641 agccgagttg cacctgcgcg agcgagacgg cggcgttggc ctgctcgacc cgcgcctcgg
     2701 cggcggtgac gtcggccttg gtgccggagt ccttggcgtt ggtgaccgca gcctgggcgg
     2761 cgatcacccc ctgctgggcc tgcttgagct gcagatcgag cgccgcggtg tcgagggtga
     2821 cgatcggctg gcccttggtc acttggtcgc cctccgcgac aggcacggtg agcacccggc
     2881 cggcgatggc cgaggagatg tcgtactgcc tggcttcgac ctgcccgttg agcgcggtgc
     2941 tggccgatgc ctcgggctgg ttggccgtgc gccaccacca ccagcctccg cccgcgagcg
     3001 cgaggacgag aacgacgatc acggggatgg gagggtgctt gcgaccgtgg cctgccatgg
     3061 acgtcacctt gcttcgtgga tcagcgggtt gaaggggggt tatttcgtgt gtgggtggca
     3121 gccgcggagg gtgacctcca cgggcacgcc tgggggcagc gggttctggg tcgtcacgtc
     3181 gacgggcacg gcccgcgtga ggtgcacctc gtcggtgacg ttggagctcg gtgggtagtc
     3241 ggcgcggtcg ccgatgaggg tgaccgtgcc ggggacggcg acgccgtcgg gcatccagtc
     3301 gccccgcacc tgggcggggt cgtcccgaca gatggtggcg agttggtccg gcgcgagcca
     3361 gacctggatg gtcgccggcc cggtgggccg gatcgtcgcg aggacggccc caggtggcag
     3421 caggtcgcct gcggaggcgg ccttgacgac cacaccggtc accggagcgg tgacggtcgt
     3481 ccgcgcctgc tgctctcggg ccagatcgac ggcaaccgca gcggtgtcgg ccatgacccg
     3541 gctgatggcc tgggcgtcga cgagttgcac ccgggcatcg gcgagggcgg cgcggccctc
     3601 ctcgacagtc gacagcccgg tgcggacctc gctcgccttc gtggcgagtt gggccagccc
     3661 ggagtcgatc tgcttgaggc ccgagcgcat ctcggtgagg ccggcgtcga gtttcgccag
     3721 gcctgcggtg gcggtggcga tccccgtggc gagtgcggcg agttgcgcgg tgagctctgc
     3781 cctggtgggc gtgccgggcg ggagcgggac gctcgggtcg gtcggcaggc ccgcgaggat
     3841 ggcgcgcagc gtggtttggg tggcggtcag gtcttcgagt gtctgcgcga gaccagcccg
     3901 attgccctgc agcgcagtct ggttcgcgtt gagctgggtg cggttggtcg tgagcgtggc
     3961 gcggctcgag gcgatcgtcg ccagcgccgt ggtgagttgg ccgtgcttgt cggtgagggt
     4021 ggctcgctgg tcgtcgacct cagcgatccg gtcagcgagc accggaactt gagcggcggc
     4081 cgtcgcggcg gtcgcttccg cagcccgcac cccagccgcg agtatgccgt cctccaccgt
     4141 cgcgatcggc tgtcccgcag tcacctggga accttctcgg actgtcaccg tcgccagtcg
     4201 ccacactccc ccgaggccgg tgatgatcac tgtcggcgag acggtgcgga cgttcgcttg
     4261 ctgagctggt gcctggctct gggctgtggt ctgcgtggca gcgaagccgg cgtcaaggtc
     4321 gacggtgggc atgccgatgg ccggcatggc gacgaccgtg agggactcct gcacggtgcc
     4381 gacgaggtgc agcgtggccg tgtcgcgcga gcaggcaccg agtgccgtcg cgaaaacagc
     4441 aagcgccgcg attgccgcga gccgagtcgc tgtcgtgggc gtggcgccac gggtggcgag
     4501 cgtcgacgag tggccggcgg gacggcatac cggacgaggg aggtgaccac tcatggtcga
     4561 tcacctgcgg gcgcgagggt ggcaaggaag gcgtcgacga cgtaccccag cgcatgggcg
     4621 tccggtaggc gcggagggcc gtcgacgagc agaccatcga ggatgacgaa gctgacgacc
     4681 gggccgatga acatgcgcgc ggtgaggcca ggctgcggcg gttcgacgac gccttcggcc
     4741 gccgccgcgc cgaggatcgc ttcgacgagc ccgaagagtt ggttggggat cacgtcgcgg
     4801 acggcgtcgc ggacctcggg cacgcgatag gcgtcgccga ggacgactcg gagcaggttg
     4861 atggtgtcgt cgcgcatgaa ccggcggacg agcgcggtcg caacggcgag cagtgtcgcg
     4921 cggagttggg ccgtgctggt gatcggcgcc ggggccccga cgtcgacgcg cagatgccca
     4981 agctcgccgt cgagaaagtc ggcgaacagc tcacccttgc tggggaagta gctgtaga
//
LOCUS       DAORPW010000166         5036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4769_length_5036_cov_751.623369, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000166 DAORPW010000000
VERSION     DAORPW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4769_length_5036_cov_751.623369"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1623)
                     /gene="dop"
                     /locus_tag="PLB86_10875"
     CDS             complement(94..1623)
                     /gene="dop"
                     /locus_tag="PLB86_10875"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HRC65684.1"
                     /translation="MGVQRVMGVETEYGVAAPGDPTANPMWLSGQVVTAYARAKGLLT
                     GRGAWDYADEAPLRDARGWELGRAWADPSQLTDIDDPTMANVVLTNGARFYVDHAHPE
                     YSGPEVTTPRAVVAWDRAGDLVMQEAARLLNPDPARPTVALYKNNTDGKGASYGTHEN
                     YLMRRETPFHDIIRHLTPFFVARQVIAGAGRVGIGQDSRVAGYQLTQRADFFETEVGL
                     ETTLKRPIINTRDEPHASADLYRRLHVIIGDANQCDVANLLKVGTTSLVLALIEDRAM
                     PGDLTVLNPVSTLHAISHDPTLRTLITLRDGRKLTALELLWIYHDACAAYLQERAGDP
                     LGGDADTHEVMTLWADVLTRLGSDPMSCARELDWVAKLRLLQAYRDRDGLAWTEPRLA
                     ALDIQWSDIRPNKSVFAKLRAAGQVTELVGAAEAQAAVTTPPEDTRAWFRGQCLSRYA
                     ANVVSASWDSVIFEVPGQTSLQRIPMLEPLRGTRAQVGEVLERSPDVATLLRELIRSG
                     D"
     gene            1732..2718
                     /locus_tag="PLB86_10880"
     CDS             1732..2718
                     /locus_tag="PLB86_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC65685.1"
                     /translation="MPLRTAIFTVVLDRFGRSSILDLDESEIATARQWAAPARWPHTW
                     VTGGVSGRVSIREAEFTARDGVERALRIYAPLYSPGPLPIVAFFHGGGYVLGNTRMYD
                     PLCAFLAKSVPAVVVSVDYRQAPEHRAPKAVEDSIDGLRWAYENAAAFRGDPARIAVC
                     GDSAGGGLAALVCHAAYDEGGPAIAHQSLLYPSTDATQSFPSAAEHGGAPVLSAEKIR
                     VFTDHYLGDSVDPADPGVSAYWRKDLHGMPPALIQTADLDPLRDEGLAYAERLADAGV
                     PVRATNYLRTVHGFMSFPGATTIGEQARYELLSELRRHLNPTRLAHLVPVDV"
     gene            2777..3760
                     /locus_tag="PLB86_10885"
     CDS             2777..3760
                     /locus_tag="PLB86_10885"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC65686.1"
                     /translation="MPRMPLRTRLLTFVLDHGTHPKITDLDVPGIQHARTRVAPLRRP
                     FTWVTGGMPPDVQTRDLWFPVRDGSQRPARAYLPSTPGPHPVIVFFHGGGWVLGSTRQ
                     YDPLCGLLAARVDALVLSIDYRMGPEHHAPQAVLDCVDATRWAATAAADLGGDPARMA
                     VCGDSAGGNLAALVCHAAYDSGGPAIAHQALIYPATDMSKYFPSVRENANAPMLTEAK
                     MDAFIAHYVGPDGLDLRDPQISPYWRADLTGLPPALVQTADIDPIRDEGLAYAARLAD
                     AGVPVRVTNYLGAVHGFASIPGATVIGKQAQHELVVELRRHLHPVTVPTLD"
     gene            3803..4810
                     /locus_tag="PLB86_10890"
     CDS             3803..4810
                     /locus_tag="PLB86_10890"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRC65687.1"
                     /translation="MADVVVFTGNAHPALAERICDHLGVKLSGVNISRFSNDCLQAQL
                     LANCRQRDVYIIQPLVPPTQEHLMELLLMIDAARGASAASITAVMPHYAYARSDKKDA
                     SRISIGGRLVADLLATAGVTRVLTMALHAPQVHGFFSVPVDHLTAIGVLADHFRQRNL
                     KDAIVVSPDLGNAKTATLFARLLNLPVAAGSKQRMADDKVVIDSIVGDVSGRRAIVLD
                     DEIATGGSIVELLERLKDFGCLEASVACTHGLFTGKAVERLRDHPMITEVVTTDTVPP
                     PLGWPQLQVRSVAELFAEAIARIHAGRSISKLFDGVDPTHGPPQPRLPFGDLAPTLPT
                     L"
     gene            4870..>5036
                     /locus_tag="PLB86_10895"
     CDS             4870..>5036
                     /locus_tag="PLB86_10895"
                     /inference="COORDINATES: protein motif:HMM:NF022700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 family protein"
                     /protein_id="HRC65688.1"
                     /translation="MPWAPVIDALLVIVFAALGRASHAEQNPVVGALATAWPFLVGLG
                     LGWGLVRWRSGR"
BASE COUNT          689 a         1810 c         1709 g          828 t
ORIGIN      
        1 cgcgccgctg cggacggctc tgctcctggc tggccatggg tcctccacct gctcgtgcgt
       61 cgatgtcggc ctcggggacc gacactgcca aacctagtcg ccggagcgga tgagttcacg
      121 cagcagcgtg gcaacgtcgg ggctgcgttc gagcacctca ccgacctggg cgcgcgtgcc
      181 gcgcaagggc tccagcatgg ggatgcgttg cagcgacgtc tgaccaggca cctcgaagat
      241 caccgagtcc cacgacgcgg agacgacgtt cgcggcatac cggctcaggc actggccgcg
      301 gaaccacgcc cgggtgtcct ccggcggggt ggtgaccgcc gcctgcgcct cggccgcgcc
      361 gaccaactcg gtgacctggc cggcggcgcg gagtttggcg aagacgctct tgttgggccg
      421 gatgtcactc cactggatgt cgagggcggc cagccgcggc tcggtccagg cgagcccgtc
      481 ccggtcgcga taggcctgca gcagacggag tttggcgacc cagtccagct cgcgggcgca
      541 gctcatcggg tcggagccca ggcgggtgag aacgtcggcc cacagggtca tcacctcgtg
      601 cgtgtcagcg tcaccgccca gggggtcgcc cgcacgctcc tgcaggtatg ccgcgcacgc
      661 gtcgtggtag atccacaaca gctccagtgc ggtgagtttg cggccgtcgc gcagcgtgat
      721 gagggtgcgc agcgtcgggt cgtgcgagat ggcgtgcagg gtcgacaccg ggttgaggac
      781 ggtgaggtcc ccgggcatgg cgcggtcttc gatgagcgcc aacacgaggc tggtcgtgcc
      841 gaccttgagc aggttggcca cgtcgcactg gttggcgtcg ccgatgatga cgtgcagccg
      901 gcgatagagg tcggcgctcg cgtgcggctc gtcgcgtgtg ttgatgatcg gccgcttgag
      961 cgtggtctcc aggccgacct cggtctcgaa gaagtcggcc cgctgagtga gctgatagcc
     1021 cgcgacccgc gagtcctgcc cgatgccgac ccggccggcg cccgcgatga cctgccgggc
     1081 cacgaagaac ggtgtcaggt gacggatgat gtcgtggaag ggcgtctcgc ggcgcatgag
     1141 gtagttctcg tgggtgccat aggaagcgcc cttgccgtcg gtgttgttct tgtagagcgc
     1201 cacggtcggc cgcgccgggt cggggttgag cagccgcgcc gcctcctgca tgaccaggtc
     1261 accggcccgg tcccaggcga caacggcgcg gggcgtggtg acctccgggc cggagtactc
     1321 cgggtgggcg tggtcgacgt agaaccgcgc cccgttggtg agcacgacgt tggccatcgt
     1381 cgggtcgtcg atgtcggtca actggctcgg gtcggcccac gcgcggccca actcccagcc
     1441 gcgcgcgtcg cgcagggggg cctcgtcggc gtagtcccac gcgcctcgcc ccgtgagcag
     1501 ccccttggcc cgcgcatagg ccgtgacgac ctggccggag agccacatgg ggttggcggt
     1561 ggggtcaccg ggcgccgcga cgccgtactc ggtctcgacc cccatgaccc gctggacacc
     1621 catgtcccca ccctatggcg gctgtcacct cctgcgatcc gggccccggt gtggtgacaa
     1681 tggagggtgc ctcgtcccac cgctcgtctc ggccgcgtcc ctcgtccccg tatgccgctg
     1741 cgcacggcga tcttcaccgt cgtcctcgac cggttcggcc gatcgtcgat cctcgacctg
     1801 gacgagagtg agatcgcgac cgctcggcaa tgggcggcgc cggcccgctg gccgcacacc
     1861 tgggtgaccg gcggggtgtc tggtcgggtg agtatccgag aggctgagtt caccgcccgt
     1921 gacggggtcg agcgggcgct gcggatctac gcccccctct actcccccgg gccgctgccg
     1981 atcgtcgcgt tcttccacgg cggcggttat gtcctgggca acacccggat gtacgacccg
     2041 ttgtgtgcct tcctcgccaa gagcgttccg gcggtcgtcg tgtcggtcga ctatcgccag
     2101 gccccggagc accgcgcgcc gaaggccgtc gaggacagca tcgacgggct gcgctgggcc
     2161 tacgagaacg ccgcggcgtt ccgcggcgat cccgctcgga tcgcggtctg cggcgacagc
     2221 gcaggcggcg ggctagccgc gctggtgtgc cacgcggcat acgacgaggg tggtccggcg
     2281 atcgcgcacc agtcgctgct ctacccgtcg accgacgcga cccagtcctt cccgtctgcc
     2341 gccgagcacg gcggcgcgcc cgtcctgagc gccgagaaga tccgcgtgtt caccgaccac
     2401 tacctcggcg actccgtgga ccccgccgac ccgggcgtct cggcctactg gcgcaaggac
     2461 ctgcacggta tgccgcccgc gctcatccag accgccgacc tcgaccccct gcgcgacgag
     2521 ggcctggcgt atgccgagcg gctcgccgat gccggcgtcc cagtgcgggc caccaactat
     2581 ttgcgcaccg tccacggttt catgagcttc ccgggggcga cgacgatcgg cgagcaggca
     2641 cggtacgagc tgctcagcga gcttcgccgc cacctcaacc ccactcggct tgcccacctc
     2701 gtcccggtcg acgtctgacc tccctcacga agttgaccgc tgcgcccggg gaggcggctt
     2761 tgacagactg acccgcatgc cccggatgcc attgcggacc cgtctgctca ccttcgtcct
     2821 cgatcacggc actcacccca agatcaccga cttggacgtt cccggcatcc agcacgcgcg
     2881 cacgcgggtc gctccgctgc gccgtccgtt cacgtgggtc acggggggta tgccgcccga
     2941 cgtgcagacg cgcgatctgt ggttccccgt ccgcgacggg tcacaacgcc cggcccgggc
     3001 ctacctcccg tcgacccccg gcccgcatcc ggtcatcgtc ttcttccacg gcggcggctg
     3061 ggtcctcgga agtacacggc agtacgaccc gctctgcgga cttctcgccg cgcgggtcga
     3121 tgccctcgtc ctgagcatcg actaccggat gggtccggag caccacgctc cccaggctgt
     3181 cctcgactgt gtcgatgcca ctcgctgggc cgccacggcg gcggccgacc tcggcggcga
     3241 cccggccagg atggccgtgt gcggggactc cgcgggaggg aacctggccg cgctcgtctg
     3301 ccacgcggca tacgactcgg gtgggccggc gatcgcgcac caagccctca tctaccccgc
     3361 aaccgatatg tcgaagtact tcccgtcggt gcgggagaac gcgaacgctc ccatgctcac
     3421 cgaggcgaag atggatgcct tcatcgccca ctacgtcggg ccggacgggc tggacctgcg
     3481 tgacccgcag atctccccct actggcgtgc cgacctcacc gggctgccgc ccgcgctggt
     3541 ccagacggcc gacatcgacc ccatccggga cgagggactg gcgtatgccg cgcgcctcgc
     3601 cgacgccggc gtgcccgtgc gagtgaccaa ctatctgggc gcggtgcacg gttttgcgag
     3661 cattcccggg gccaccgtca tcggcaagca ggcccaacac gagttggtcg tcgagctgcg
     3721 tcggcacctg catccggtga ccgtgccgac gctggactag ggtgtccttg cacagttcaa
     3781 ccgttgcagc atgggggtga ttgtggccga cgtcgtggtg ttcaccggta acgcacaccc
     3841 cgcgctcgcc gagcggatct gtgaccattt gggggtcaaa ctctccgggg tcaacatcag
     3901 ccggttctcc aacgactgtc tgcaggccca gttgctggcc aactgtcgcc agcgtgacgt
     3961 ctacatcatc cagccgctcg ttccgccgac gcaggagcac ctcatggagc tcctcctcat
     4021 gatcgatgcg gcgcgcggag cctcggcggc ctcgatcacg gcggtcatgc cccactacgc
     4081 ctatgcccgc tcggacaaga aggacgcctc ccgaatttcc atcggcggcc ggttggtggc
     4141 cgacctcttg gccaccgccg gggtgacacg cgtgctgacc atggcgctgc atgcgccgca
     4201 ggtccacggc ttcttctcgg tgccggtgga tcacctgacg gccatcggcg tcctggccga
     4261 ccacttccgt cagcgcaacc tcaaggacgc gatcgtcgtc tcccccgacc tcggcaacgc
     4321 caagacggcc accctcttcg cccggctgct caacctgccg gtcgcagcgg gcagcaagca
     4381 gcggatggcc gacgacaagg tcgtcatcga ctccatcgtc ggcgacgtgt ccgggcgtcg
     4441 cgccatcgtc ctggacgacg agatcgcgac tggtgggtcg atcgtcgagt tgctcgaacg
     4501 gctcaaggac ttcgggtgtc ttgaggcgtc ggtcgcctgc acgcatgggc ttttcaccgg
     4561 caaggcggtc gagcggttgc gcgaccaccc gatgatcacc gaggtcgtca cgaccgatac
     4621 cgtgccgccg ccgctgggct ggccgcagtt gcaggtgcgc tcggtggctg agctcttcgc
     4681 cgaggcgatc gcccgcatcc acgcgggccg ctccatctcg aagttgttcg acggtgtcga
     4741 ccccacccac gggccgccgc agccgcgcct ccccttcggt gacctcgccc cgaccctgcc
     4801 gacgctgtga gcgagcagcc cggctcggca ggcggcggtc gcgggtcagt cgcctccgcc
     4861 cttctcggta tgccgtgggc gcccgtgatc gacgctcttc tggtcatcgt gttcgcggct
     4921 ctgggacgcg cgagccacgc cgagcagaac cccgtcgtcg gagccctcgc gacggcctgg
     4981 cccttcctcg tgggtctcgg cctgggctgg ggactggttc gctggcggtc gggacg
//
LOCUS       DAORPW010000167         5033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4782_length_5033_cov_673.713138, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000167 DAORPW010000000
VERSION     DAORPW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4782_length_5033_cov_673.713138"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLB86_10900"
     CDS             <1..1035
                     /locus_tag="PLB86_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRC65689.1"
                     /translation="WDEVAPIIEIVDARSAAEGGPRMTFFEVLVVLAYAVFADAPVDV
                     AVVEVGMGGRWDATNVADGDVAVVTPIDIDHTHFLGSTVEEIAFEKAGIVKPGAVLVT
                     AAQDEAVAAILTDRCDEVGARRYAADEDFAVTDREVALGGQLLAIRGVAGNYDEIFLP
                     LHGEHQGANAALALVAVELFLGGGERRLDPEVVRAGFAAVTSPGRLEVVRRSPTILVD
                     AAHNPHGATALALALGEAFSFARLVGVVAIFADKDAGAMLEALEPALDHVVVTRNSSP
                     RSMNVASLGEIASGIFGAERVSVVPALPDALEEAVTVAESEGMGGGVVVTGSVVTAAD
                     VRLLLGVKET"
     gene            1035..1433
                     /locus_tag="PLB86_10905"
     CDS             1035..1433
                     /locus_tag="PLB86_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HRC65690.1"
                     /translation="MAGLVFFGTLGKFTWRMCAAVLAAQSIAVFFGALVARGIAVADS
                     SGAATAYLLVGSGLAILCVIAAGAMRSPFGVTLGWLIQVATFAATVIVPLMGLVGVIF
                     GALWVLCLIQGHRIDGLQRAHAQESAAPEE"
     gene            1444..1866
                     /gene="ndk"
                     /locus_tag="PLB86_10910"
     CDS             1444..1866
                     /gene="ndk"
                     /locus_tag="PLB86_10910"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRC65691.1"
                     /translation="MTTERSLVLVKPDGFARGLTGEVIRRIERKGYRLVALRILTPTR
                     EMLGRHYAEHAEKPFFGELVDFMSSGPVTAIVIEGNQCVPGFRSLAGATDPTKAAPGT
                     IRGDLGRDWGKGSTENVVHGSDSPESAAREMAIWFPDL"
     gene            complement(1873..2325)
                     /locus_tag="PLB86_10915"
     CDS             complement(1873..2325)
                     /locus_tag="PLB86_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRC65692.1"
                     /translation="MIPLDELVGRAEALARSAHAGQVDKAGAPYAQHPERVAAAVTGD
                     PQAQAAAWLHDVVEDTSTSLADLRAAGFPEPVIAAVDALTRRPDEPGDNYYARVAADP
                     LALRVKRADIADNSSPERLGRLDEATQIRLRHKYAHALATLAAYELPR"
     gene            2468..3589
                     /locus_tag="PLB86_10920"
     CDS             2468..3589
                     /locus_tag="PLB86_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HRC65693.1"
                     /translation="MAALAFRDPRLEAAVERAWARSRLSLRTRVDRLVDKSWHVVQCA
                     VAAGVAWWFASGVLHHAMPFFAPIVAVVCLGMTYGQRLRRVAEVTVGVAVGIAVADIF
                     VGVVGSGPWQITVVVLVSMSLALLLDAGLLLVMQSAVQSIVVTVLVPTGSQALGRWVD
                     AVIGGVVALVFAMVVPRAPLRRPRRQAARVGRVMARLLREAAQSAREGDVERSAQVLA
                     DARQTDPLIRELQAAADEGMSVIASTPFTRTDAGSVRSIASVVDPLDRAMRSTRVLVR
                     RMSIAADSDIEVPDDYVDVIVALADAVDILDRVWAENRSAEFARPGFVALAERTATLD
                     PGEYHTTVILAQLRSLVVDLLELSGMDHEDAVAAVPPLG"
     gene            complement(3679..4674)
                     /gene="rfbB"
                     /locus_tag="PLB86_10925"
     CDS             complement(3679..4674)
                     /gene="rfbB"
                     /locus_tag="PLB86_10925"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRC65694.1"
                     /translation="MKVLVTGGAGFIGANFVLRVRETRPDWEVTVLDALTYAGNRASL
                     APVEGQIEFVQGSIADADLVDRLVADTDLVVHFAAESHNDNSLDNPWPFMEANIIGTY
                     RLLEAVRKHGKRIHHISTDEVYGDLELDDPNRFSESTPVNPSSPYSASKASADLLVRA
                     WIRSFKIAATLSNCSNNYGPRQHVEKFIPRQITGILDGVKPKLYGAGANVRDWIHVDD
                     HNDAVIAIIERGRLGETYLIGADGERNNREIIALLLELMGKPADWFDHVNDRPGHDLR
                     YAIDSSKLRAETGWTPVYQDIHAGLRQTIDWYAANEAWWRTAKESTEQVYARLGR"
     gene            complement(4671..>5033)
                     /locus_tag="PLB86_10930"
     CDS             complement(4671..>5033)
                     /locus_tag="PLB86_10930"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HRC65695.1"
                     /translation="HEWVPEEFAGRLARADAADPGLWASLTRDGHSIATVHQTITAGL
                     ASGADADLLHTAVGVPLLLVRRVAMGADGIPLALSDHRYLAHRFALEVEFNSGPSASS
                     PEPPGLRAVADPPQEIPS"
BASE COUNT          666 a         1636 c         1849 g          882 t
ORIGIN      
        1 tgggacgagg tggccccgat catcgagatc gtcgacgcgc gttcggctgc cgagggtggc
       61 ccccggatga cgttcttcga ggtcctcgtc gtgctcgcct acgccgtctt cgccgacgcg
      121 cccgtcgatg tggcggtcgt cgaggtgggg atgggtggcc gttgggatgc gaccaacgtg
      181 gcggacggcg atgtcgccgt cgtgacgccg atcgatatcg atcacaccca cttcctcggc
      241 tcgaccgtcg aggagatcgc cttcgagaag gcggggatcg tcaagccggg tgccgtcctg
      301 gttacggctg cccaggacga ggcggtcgcc gcgatcctca ccgaccgctg tgacgaggtc
      361 ggagcccgcc ggtatgccgc ggacgaggac ttcgcggtca ccgaccgtga ggtggccctc
      421 ggaggccagt tgctggcgat ccgcggggtg gccggcaact acgacgagat cttcctgccc
      481 ctgcatggcg agcaccaggg cgccaatgcc gccttggccc tcgtcgccgt cgagctcttc
      541 ctcggcggag gtgagcgacg cctggacccc gaggtggtcc gcgccgggtt cgccgcggtg
      601 acctccccgg gccggctcga ggtcgtgcgt cggtcgccga cgatcctcgt tgacgcagcc
      661 cacaacccgc atggggccac ggccttggcg ctggccctgg gcgaggcgtt ctccttcgcc
      721 cggttggtcg gcgtcgtggc gatcttcgcc gacaaggacg cgggcgccat gttggaggcg
      781 ctcgagccgg cccttgacca tgtggtcgtc acccgcaaca gctcgccccg ctcgatgaac
      841 gtggcttctc tgggcgagat cgcctcgggc atcttcggcg ccgagcgggt gtcggtcgtc
      901 cccgcccttc ccgacgcgct cgaagaagcg gtgaccgtcg ccgagtcgga gggcatgggc
      961 gggggagtcg tcgtgaccgg ttcggtcgtc accgcggccg acgtgcggct gctgctcggg
     1021 gtgaaggaga cctgatggct ggcctcgtgt tcttcggcac cctgggcaaa ttcacctggc
     1081 ggatgtgcgc ggccgtcctt gcggcgcagt cgatcgcggt cttcttcggt gccttggtgg
     1141 cccgcgggat tgccgtcgcc gacagcagcg gggcggcgac ggcatacctg ctggtcggaa
     1201 gcggcctcgc aatcctctgc gtcatcgcgg ccggggcgat gcgctcgccg ttcggggtga
     1261 ccctcggctg gctgattcag gtggcgacct tcgcggcgac cgtgatcgtc ccactcatgg
     1321 gcctggtcgg cgtcatcttc ggggcgctgt gggtgctgtg cctcatccag ggacaccgca
     1381 tcgacgggtt acagcgcgca cacgcgcagg agtctgccgc cccggaggaa tagggtgggc
     1441 cgcgtgacta ctgagcgctc cctggtcctg gtcaagcccg acggtttcgc ccgcggcctt
     1501 accggtgagg tgatccgtcg aatcgaacgc aagggctacc gcctggtcgc cctgcgcatc
     1561 ctcacgccca cccgcgagat gctcggccgg cactacgccg agcacgccga gaagccgttc
     1621 ttcggtgagt tggtcgactt catgtcctcc ggccccgtga cggcgatcgt catcgagggc
     1681 aaccagtgcg tcccgggctt ccggtcgttg gctggcgcga ccgaccccac caaggcggcg
     1741 ccgggcacga tccgtggaga cctcgggcgc gactggggca agggctcgac cgagaacgtc
     1801 gtccacggct ccgactcccc ggagtcggcc gcccgcgaga tggcgatctg gttccccgac
     1861 ctctgagtcg cgtcaccgcg gcagttcgta tgccgcgagc gtcgccaacg cgtgtgcgta
     1921 cttgtgccgc aggcggatct gggtagcctc gtccaggcga ccgagtcgct cggggctgct
     1981 gttgtccgcg atgtcggctc gcttgacccg caacgccaac gggtcggccg cgacccgggc
     2041 gtagtagttg tcgccgggct cgtcggggcg ccgggtcagg gcatcgaccg cggcgatcac
     2101 cggctcgggg aagccggcgg cacgcaggtc ggccaacgag gtggacgtgt cctcgacgac
     2161 gtcatgcaac caggccgccg cctgagcctg cgggtcacct gtcacggccg ccgccacccg
     2221 ttccgggtgc tgggcatagg gtgcgccggc cttgtcgacc tggccggcgt gggccgaccg
     2281 agcgagcgct tcggctcgcc ccacgagctc atccagaggg atcatccgtt ggcctttcga
     2341 cgtcgcgtcg ggtcgggtcg gggcgagccg cgcggcatac ctgcggccca tccgagccgc
     2401 atccctcgcc gatcacggct cgtggccagc ctacgggcgg gtccttggga gcgaggagaa
     2461 tggacccatg gccgcccttg cattccgtga ccctcgactc gaagccgccg tggagcgggc
     2521 ctgggcgcgc agtcgactgt cgttgcggac ccgggtggac cgcctcgtcg acaagagctg
     2581 gcatgtcgtg cagtgcgccg ttgctgccgg ggtcgcctgg tggttcgcct cgggcgtgtt
     2641 gcaccacgcg atgccgttct tcgcccccat cgtcgccgtg gtctgcctgg gcatgaccta
     2701 tgggcaacgg ctgcgccggg tcgcggaggt gacggtcggc gtcgccgtcg gcatcgccgt
     2761 cgccgacatc ttcgtggggg tcgtcggtag cggcccgtgg cagatcaccg tcgtcgtgct
     2821 cgtgtcgatg tcgctggcgc tgctgctgga cgcggggttg ttgctcgtca tgcaatctgc
     2881 cgtgcagtca atcgttgtca ccgttctcgt ccccacgggt tcgcaggcgt tgggccgatg
     2941 ggtcgatgcc gtgatcggag gagtggtcgc cctcgtcttc gccatggtcg tgccccgagc
     3001 gccgctgcgt cgccctcgcc gtcaggccgc ccgggtcggc cgggtgatgg cccggctgct
     3061 gcgtgaggcg gcccagagcg cgcgggaggg agatgtcgag cgctcggcgc aggtgctcgc
     3121 cgacgcccga cagaccgatc cgctcatccg cgagttgcag gccgccgccg acgagggaat
     3181 gtcagtcatc gcctcgactc cgttcacccg cacggacgcc ggcagcgtgc gcagcatcgc
     3241 ctctgtcgtc gacccgttgg accgggccat gcgcagcacc cgggtgttgg tgcgtcggat
     3301 gagcatcgcg gccgacagcg acatcgaggt gccggacgac tacgtggacg tcatcgtcgc
     3361 gttggccgat gccgtcgaca tcctcgaccg ggtctgggcg gagaaccgct ccgcggagtt
     3421 cgcccggcca ggtttcgttg cgctggccga acggaccgcg acgctggatc cgggggagta
     3481 ccacacgacg gtgatcctcg cgcagttgcg ctcgctcgtc gtggacctgc tggagctcag
     3541 cggcatggac cacgaggacg cagtggctgc cgtcccaccg cttggatgag gctagtgaca
     3601 cttgtatgag ggtgcagcct cctacaagtg acatcaacct catccaagcg ggtgatgtgc
     3661 ccgtgggggg tgaggcggtc agcggccgag gcgcgcgtag acctgctcgg tgctttcctt
     3721 ggccgtgcgc caccaggctt cgttggcggc ataccagtcg atggtctggc gcagccccgc
     3781 gtggatgtcc tggtagacgg gggtccaccc ggtctcggca cgcagcttgc tggagtcgat
     3841 ggcgtagcgc aggtcatggc cggggcggtc gttgacgtga tcgaaccagt cggccggctt
     3901 gcccatgagt tcgagaagca gcgcgatgat ctcgcggttg ttgcgttcgc cgtcggcccc
     3961 gatgaggtag gtctcgccga ggcggcctcg ctcgatgatg gcgatgacgg cgtcgttgtg
     4021 gtcgtcgacg tgaatccagt cgcggacatt ggcgccggct ccatagagct tcggcttgac
     4081 gccgtcgagg atgccggtga tctggcgggg gatgaacttt tcgacgtgct gacgcggccc
     4141 gtaattgttc gagcagttcg agagggtcgc ggcgatcttg aaggaccgga tccaggcgcg
     4201 gacgagcaga tcggcgcttg ccttgctcgc cgaatagggc gaggaggggt tgaccggggt
     4261 ggattcggag aaccggttgg ggtcatcgag ctcaaggtcg ccgtagacct cgtcggtcga
     4321 gatgtggtga attcgcttgc cgtgcttgcg gactgcttcg agcaaacggt aggtcccgat
     4381 gatgttggcc tccatgaacg gccacgggtt gtccagcgag ttgtcgttgt gtgactcggc
     4441 ggcgaagtgc accaccaggt cagtgtcggc aacgaggcgg tcgacgaggt cggcgtcggc
     4501 gatcgagccc tggacgaact cgatctgccc ctcgacgggg gcgagggagg cccggttgcc
     4561 ggcatatgtc agcgcgtcca gaacggtcac ctcccagtcg ggtcgggtct cgcgcacgcg
     4621 caggacgaag ttggcaccga tgaatccggc gccgccggtg acgaggacct tcatgacggg
     4681 atctcctggg gtgggtcggc cacggcccgc aggccggggg gttctgggga gctggcggac
     4741 gggcccgagt tgaactccac ctcgagcgcg aatcgatggg cgagatagcg gtggtcggac
     4801 agggcaaggg gtatgccgtc cgcccccatc gcgacgcggc gcaccagcag gagcggcacg
     4861 ccgacggcgg tgtgcagcag gtcggcatcg gccccgctgg ccaggccggc ggtgatcgtc
     4921 tggtgcaccg tggcgatgga gtggccatcg cgggtcaggc tggcccagag gccggggtcg
     4981 gcggcatcgg cccgcgccag gcgccccgcg aactcttccg ggacccactc gtg
//
LOCUS       DAORPW010000168         5020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4807_length_5020_cov_246.152266, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000168 DAORPW010000000
VERSION     DAORPW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4807_length_5020_cov_246.152266"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PLB86_10935"
     CDS             <1..877
                     /locus_tag="PLB86_10935"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRC65696.1"
                     /translation="ICCAAAPESVEGIPVVGCEEDAAELAQRVGADVVAYAASSRLGA
                     TGLRKLGWALEGTGIDLVVAPGMTDVAGPRIHARPVAGLSLLYVEAPRFTGTRLLLKS
                     AFDMLAAFILMVALAPLFVVLSALVWFEDHGPVFYTQRRVGHGGRSFVMTKFRSMTVG
                     ADQVLPEGNDADGPLFKLRDDPRVTRIGRFLRRSSLDELPQLLNVLRGEMSLVGPRPP
                     LESEVAAYEEDVHRRLLVKPGLTGLWQVSGRSDLSWEDSVRLDLYYVENWSFTSDLRI
                     LMRTLRAMHESNGAY"
     gene            complement(897..3170)
                     /locus_tag="PLB86_10940"
     CDS             complement(897..3170)
                     /locus_tag="PLB86_10940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC65697.1"
                     /translation="MTELVVLSLEAWDEVWRRNQHLVAGLLRTDPDLRVLFVEPAADP
                     VYAALRARVLPRSGVGLRRGPRLPEVDGDRLWLLQPTKWLPRKVDSRADARLAGAVVA
                     AAREVGFSDPVLWVNDPAGAGTLAATGWRALYDITDDWLLAPRTPAEHARLVADEAML
                     LARSVEVVVCSPALAASKAATRPVRLVPNAVDLAAYQRDSARPADLPDGAVALYVGTV
                     HTDRMDLDLLAATARALAGTASVVCVGPAPLAPTDATAVTAAGVRLLGPRPAETIPAY
                     LRHADVLVVPHAVTPFTDSLDPIKRYEYQAAGRPVVATPVAGFADSGMPRLTVAARAD
                     FPAAVAVAVAAAVAAEFEVTQALPPVDASVPTWAERVDAMSAVVNTVVASRDSRRRPP
                     VAIAHDYLTQRGGAERVVLAMLTAFPDATVYTTLYDPAGTYPEFAEARIVTSPLDRIG
                     ALRRHHRAALPLLAWGTGRLHPDADIVVVSSSGWAHGVRSTGRRIVYCHAPARWLYQT
                     EAYLGAAGRWAPARLGLATLRPALLRWDRRAARSADRYLANSRVVRDRVRAAYGIDAT
                     VLPAPHAVGTEGQREPVATLTDWAEGGFHLIVSRLLPYKNVDVVAAAFAGMPSRRLVV
                     VGDGPGRAALARDLPGNVRLVSGLRDAQLRWVYAQARALVAPSLEDYGLTPLEAAAFG
                     VPTLALEAGGYLDTVAPGVSGLFFPEPTPAAVAEAVEAGERHTWSAEAIRAHAALFSA
                     EVFVAALRAHVDALRST"
     gene            complement(3167..4207)
                     /locus_tag="PLB86_10945"
     CDS             complement(3167..4207)
                     /locus_tag="PLB86_10945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC65698.1"
                     /translation="MTRVVHVVRSDGFAGVEGFIARLAAEQARAGHQVSVIGGEVSRM
                     RGALGPTVPHTPDETVLGVVRSLLALPQPPDVLNVHMTAAELAATVAAALRRWGARPA
                     VVATCHFAGRRGSGSRAFGPLVAAVARHRVVAQIAVSRFVAAEVGGESDVVHTGVPNR
                     AMPDGPRDRTVLVVQRLEPEKRTTDALRVFAESGVAHRGWRLQVAGDGVDRGAVVSLA
                     AELGIAASTDLLGRRDDVPTLMSQAGILLAPTPREGLGLAVVEAMAAGLPILAAGSGG
                     HLETVGSIPEGQLYTDLTDAAERLGALADDEGGRAAYGAALWAAQREKFTLARQAAAT
                     EAVYRRALEARS"
     gene            complement(4204..>5020)
                     /locus_tag="PLB86_10950"
     CDS             complement(4204..>5020)
                     /locus_tag="PLB86_10950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC65699.1"
                     /translation="FARVLRNPLHPFTITRDLMRYRSRRIHQVVVNLTSTDAAALQHT
                     YGRVRPRSVVIGNGVDTDRFRPPTEQERSVARARLAIPPDQPAVLFVGHEFGRKGLPE
                     LVDAIAGLVAPVHLVVVGGTPDLIADIARHPAAKSLGARLHLLGPLADPLWVFHGADV
                     FVLASAYEAYPLVVLEALATGLPVVATPVGPVPDLVVNGHNGQIVDRTPEAIRAGIDR
                     VLSGDPAGLAEAARATALEHTWGAVADRYLVLLRELAQVSPPDRGNEPDGAGR"
BASE COUNT          783 a         1759 c         1814 g          664 t
ORIGIN      
        1 catctgttgc gcggccgcgc cagagtccgt cgagggcatc cccgtggtcg gctgcgagga
       61 ggacgccgcg gagttggctc agcgggtcgg cgccgacgtc gtggcgtatg ccgcctcgtc
      121 gcgcctgggt gccaccggcc tgcgaaagct gggctgggcc ttggagggca ccggcatcga
      181 tctcgtcgtc gcgcccggca tgaccgatgt cgccggccct cggatccatg cgcgacccgt
      241 cgccggcctg tcgctgctct atgtcgaggc accccgcttc acggggaccc gcctgctgct
      301 caagtcggcc tttgacatgc tcgctgcgtt catcctcatg gtcgcgctcg cgccactgtt
      361 cgtcgtcctc agcgccctcg tctggttcga ggaccatggg ccggtgttct acacccagcg
      421 tcgggtcggg cacggcggtc gcagtttcgt catgacgaag ttccgctcca tgacggtcgg
      481 tgccgatcag gtactgccgg agggcaacga cgccgacggg ccgctgttca aactgcgtga
      541 cgacccccga gtgacccgga tcgggcggtt cctgcgccgg tcgagcctcg atgagctccc
      601 ccagttgctg aacgtcctgc gcggtgagat gagcctggtc gggccgcgtc cgccgctgga
      661 gagcgaggtc gccgcctacg aagaggacgt gcatcgtcgg ctgctcgtca agcccgggct
      721 cacggggctc tggcaggtga gcggtcgcag cgacctgtcg tgggaggaca gcgtccgatt
      781 ggacctctac tacgtggaga actggtcttt cacgtccgac ctgcgcatcc tcatgcgcac
      841 gctgcgcgcc atgcacgaga gcaacggcgc ctactgactc agccggtgat cccggctcag
      901 gtgctccgca acgcatctac gtgggctcgc aacgctgcca cgaacacctc ggccgagaag
      961 agcgccgcat gggcgcggat cgcctcggcc gaccaggtat gccgctcccc cgcctcgacc
     1021 gcttcggcca ccgccgccgg ggtcggctcc gggaagaaca acccgctgac gcccggcgcc
     1081 accgtatcga gatagccgcc agcctccagc gccagggtcg gcaccccgaa ggcggccgcc
     1141 tccagcgggg tgaggccgta gtcctccagg ctcggggcca cgagcgcccg cgcctgggca
     1201 tagacccagc ggagttgggc gtctcgcagc cccgacacca gacgcacgtt gcccggcagg
     1261 tcgcgggcga gggcggcgcg cccgggcccg tcaccgacga cgaccaggcg gcgggacggc
     1321 ataccggcga acgcggcggc cacgacgtcg acgttcttgt agggcaacag ccgcgacacg
     1381 atgaggtgga agcccccctc ggcccagtcg gtcagggtcg cgaccggctc gcgctggccc
     1441 tcggtgccca cggcgtgcgg agcgggaagg accgtcgcgt cgatcccgta tgccgcccgc
     1501 acccgatccc ggacgacccg cgagttggcg aggtaacggt ctgcagaccg ggcggcccgc
     1561 cggtcccagc gcagcagcgc cgggcgcagg gtagccaggc cgagccgggc gggagcccat
     1621 cggccggccg cgccgaggta ggcctcggtc tggtagagcc agcgagcggg cgcgtgacag
     1681 taaacgattc gccgcccggt cgagcggacc ccatgagccc acccactgct ggacaccacg
     1741 acgatgtcgg cgtccggatg caaccggccg gtcccccaag cgagcagcgg gagtgcagcg
     1801 cgatggtggc gacgcagagc gccgatccgg tcgagcgggg aggtgacgat gcgggcctca
     1861 gcgaactcgg ggtaggtgcc ggctgggtcg tagagcgtcg tgtagacggt cgcgtccggg
     1921 aaggcggtga gcatcgcgag gacgacccgc tccgccccac cccgctgggt gagatagtcg
     1981 tgggcgatcg cgacgggggg gcgacgtcgc gaatcccggc tcgccaccac ggtattcacc
     2041 accgcgctca tcgcgtcgac ccgttcggcc caggtgggga ccgacgcatc gaccggcggg
     2101 agtgcctggg tcacctcaaa ctcggcggcg accgcggcgg caactgcgac ggcgaccgcg
     2161 gcagggaagt cggcgcgggc tgccacggtg aggcgcggca taccggagtc ggcgaagccg
     2221 gccacgggag tggcgacgac gggtcggccg gccgcttggt attcgtagcg tttgatcggg
     2281 tcgaggctgt cagtgaaggg cgtgacggcg tgcgggacga cgagcacgtc ggcgtggcgc
     2341 aaataggccg ggatcgtctc ggcggggcgc ggcccgagca accggactcc ggcagccgtc
     2401 actgctgtcg cgtcggttgg cgcaagcggc gcggggccga cgcacacaac gctcgccgtc
     2461 ccggcgaggg cgcgtgccgt cgctgccagg aggtccaggt ccatccggtc ggtgtggacg
     2521 gtcccgacgt agagcgcgac cgcaccatcc ggcaggtcgg caggtcgggc cgaatcacgt
     2581 tgataggcag cgagatccac ggcgttgggc acgagccgga caggccgggt cgcagccttc
     2641 gaagcggcca gggccggcga acagacgacc acttcgacgg accgggcgag cagcatcgcc
     2701 tcgtcggcga ccaaccgggc gtgctcggcc ggggtccggg gagcgagcag ccagtcgtcg
     2761 gtgatgtcgt agagcgcccg ccatccggtg gccgcgagcg tccccgctcc cgccggatca
     2821 ttgacccata gcaccgggtc gctgaacccg acctcgcgcg cggccgcgac gacggcgccg
     2881 gccagccggg cgtctgcacg cgaatcaacc ttgcgcggaa gccatttcgt cggctgaagc
     2941 agccagagcc ggtcaccgtc gacctccggc agccgggggc cgcgacgcag ccccacgccc
     3001 gagcgaggga gcacgcgagc ccgcagcgcg gcatacaccg ggtcggcggc cggctcgacg
     3061 aagaggaccc gcaggtcggg gtcagtgcgc agcaaccccg ccacgaggtg ctggttgcga
     3121 cgccacacct cgtcccaggc ttcgagggac agtacgacga gctcggtcac gagcgggcct
     3181 ccagtgcacg ccgatagacc gcctcggtcg ccgcggcctg ccgcgccaag gtgaacttct
     3241 cgcgttgggc cgcccacagg gccgccccgt atgccgccct cccgccctcg tcgtcggcca
     3301 gggcgcccag ccgctcggcg gcgtcggtga ggtcggtgta gagctgaccc tcggggatcg
     3361 acccgacggt ctccaggtgc ccgcccgagc cggctgccag gatgggcagc ccagcggcca
     3421 ttgcctcgac gacagccagg ccgaggccct cccgaggcgt gggggcgagc aagatcccgg
     3481 cctgggacat cagggtcggc acgtcatcgc gccggcccaa cagatcggtg ctcgcggcga
     3541 tccccaactc ggcggccagg ctcaccactg cccctcggtc gacgccgtcg cctgcgacct
     3601 gcaaccgcca accgcggtgc gccacgcccg attcggcaaa gacgcgcagg gcatccgttg
     3661 tgcgcttctc cggttcgagg cgctggacga cgagaacggt ccggtcgcgc ggaccatccg
     3721 gcatcgcgcg gttcggcacc cccgtgtgga cgacgtccga ctcaccgccg acctccgcag
     3781 ccacgaagcg actgaccgcg atctgtgcga caactcggtg ccgcgcgacg gcagccacga
     3841 gtggaccgaa agcccgcgag cccgacccac gccgtccggc gaagtgacac gttgcgacga
     3901 ccgcggggcg agctccccag cgccgcaacg ctgcagcaac ggtcgcggcc agctcggctg
     3961 cggtcatgtg gacgttgagc acatccggcg gctggggtag cgcgagaagg cttcgaacga
     4021 cgcccaggac cgtctcatcg ggggtgtgcg ggaccgtcgg gccgagggca ccgcgcatcc
     4081 tggatacttc gccgccgatg acgctgacct gatgtcctgc ccgggcctgc tcggccgcca
     4141 accgggcgat gaatccctcg acccccgcga agccgtcaga gcggaccacg tggacgaccc
     4201 gagtcatcgc cccgctccgt cgggctcatt gcctcgatcg ggcggggaga cctgtgccaa
     4261 ttcccggagg aggacgagat agcgatcggc gaccgcaccc caggtgtgtt ccagcgcggt
     4321 ggcacgggcg gcctcagcca acccggccgg atcaccggac aacacccgat caatgcccgc
     4381 acggatggcc tcaggcgtcc ggtcgacgat ctggccgttg tgtccgttga caacgagatc
     4441 tggaaccggc cccaccgggg tggctacgac cggcaggccg gtggccaagg cttccaggac
     4501 gacgagcggg taggcctcat atgcgctggc caggacgaac acgtcggcgc catggaacac
     4561 ccagagaggg tccgccagtg gaccgagcag gtgcaacctg gccccgagtg acttcgccgc
     4621 ggggtgccgc gcgatgtcgg cgatcaggtc cggggtgcct ccaacgacga ccaggtgcac
     4681 cggcgccacc aggccggcga tggcgtcgac gagctcagga agacccttgc gaccgaactc
     4741 atgccctacg aagagcacag cgggttggtc gggggggatc gcgagccggg cccgggcgac
     4801 agacctctcc tgctcggtcg gtggacggaa ccggtcggtg tcgacgccgt tgccgatgac
     4861 gacgctgcgg ggccgcaccc gtccataggt gtgttggagc gcggctgcgt cggtcgaggt
     4921 gaggttgaca acgacctgat ggatacgccg agatcgatac cgcatcaggt cacgcgtgat
     4981 ggtgaaggga tgaagggggt tgcgcaggac ccgagcgaac
//
LOCUS       DAORPW010000169         4990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4861_length_4990_cov_835.628166, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000169 DAORPW010000000
VERSION     DAORPW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4861_length_4990_cov_835.628166"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLB86_10955"
     CDS             complement(<1..896)
                     /locus_tag="PLB86_10955"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRC65700.1"
                     /translation="MARRGANTKVLAIVLAGGEGKRLMPLTRDRAKPAVPFGGIYRLI
                     DFALSNVVNSGYLKVVVLTQYKSHSLDKHVTKTWRMSTILGNYVAPVPAQQRVDKNWY
                     LGSADAIFQSLNLVDDEKPEIVVVVGADHVYRMDFSQMVQQHIETGAALTVAAIRQPI
                     HMADQFGVIEVDPTDTRKIGAFREKPTDPVGLPDAPHEILASMGNYVFTASKLVQAVT
                     EDHDLEGSKHDMGGDIVPRFVRESDAYVYDFKDNDVPGATERDRGYWRDVGTMDSYYD
                     AHMDLISVHPIFNLYNYDWPIYT"
     gene            994..>2180
                     /gene="glgA"
                     /locus_tag="PLB86_10960"
     CDS             994..>2180
                     /gene="glgA"
                     /locus_tag="PLB86_10960"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HRC65701.1"
                     /translation="MRVDIISKEYPPAIYGGAGVHVAELVRALRDRGDVDVQVRCFGA
                     PRDEAGTTAYPDLPELASANPALQTFGVDVTIAQDCQGADIVHSHTWYANLAGHLGGL
                     LAGAPHVVTAHSLEPMRPWKAEQLGGGYRVSSFAERAAYEGAAAVIAVSAGMRADILR
                     SYPSLDPAKVPVVHNGIDSQMWQPIHDADVCRKHGMDPDRPAVTFVGRITRQKGLPYL
                     MRAAARLPEEVQVVLCAGAPDTPEIKAEVEGLLADLRAQRDGVVWIPEMLPRSEVISL
                     LTASTVFACPSVYEPLGIVNLEAMACELPVVATATGGIPEVVVPGETGWLVPIEQVQD
                     GTGTPVDPEKFVGDLAEALTNAVSDRERARTMGLAGRKRAVEQFSWESIGDKTMAVYQ
                     DVLG"
     assembly_gap    2181..2280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2304..3062)
                     /locus_tag="PLB86_10965"
     CDS             complement(2304..3062)
                     /locus_tag="PLB86_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65702.1"
                     /translation="MLALPIVVRCALWGTRWLGGRGDLDDVVGNVHSGAATDIAGPFK
                     ATLRDWAERGETAVFLALPRPGLLTGMPRAPLAVTAAATDAGQAAYAPTVGGALVPAL
                     TRFGPEGDTGVLATWAAYETEPVPRHIAEAPDVGDLSRRLTEAVLEATTRLATVGGIP
                     WRGGEATPPPRASVPGLPPGLAPRPLHLLDRAGDVRALALAGLGVEESGAALDASTSA
                     ERTAVLRRLLTEAEATLVGATNVLSMSVAGWRPA"
     gene            3202..4011
                     /locus_tag="PLB86_10970"
     CDS             3202..4011
                     /locus_tag="PLB86_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65703.1"
                     /translation="MSDVLAFAGVSVVRGHNRLLDDVTWEVEEGERWVILGPNGAGKT
                     TLLQIAAARMHPTTGVVGILGEVLGTVDVFELRPRVGLASASIAERIPGGESVGNVVV
                     TASYGIIGRWRESYDRQDYARAMGLLDALGVAHLADRLYGTLSEGERKRVQIARALMT
                     DPELMLLDEPAAGLDLGGREDLVARLGVLAEDTEAPALVLVTHHVEEIPPGFTDVLLL
                     REGRIVAAGPLPLALTSANLSATFGLPLVVERHGKRWSARAADPESTPSTG"
     gene            4098..4559
                     /locus_tag="PLB86_10975"
     CDS             4098..4559
                     /locus_tag="PLB86_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRC65704.1"
                     /translation="MFQGSSWLIWVGVALALGAIEAATVDFIFLMLAGGALAGAVAAA
                     FGADFPGQAIVASVSAVALLVGVRPWVKRRFNVKTERPTVGIAGNVGRTATVLETVTL
                     AGGRINLSGETWSARSQGGDAILPGDKVEVIAIDGATAVVAPVTLPPQPLP"
     gene            4632..>4990
                     /locus_tag="PLB86_10980"
     CDS             4632..>4990
                     /locus_tag="PLB86_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRC65705.1"
                     /translation="MPRIETTIVLLLLLVFAITVVVRTIRIVPQQTAVIIERLGSYSR
                     TLDDGMHVLVPFIDKPRATIDLREQVVTFPPQPVITSDNLVVSIDTVIYYTVTDAKSA
                     VYEIANFIQGIEQLTVTT"
BASE COUNT          721 a         1654 c         1711 g          804 t
ORIGIN      
        1 gtgtagatcg gccagtcgta gttgtagagg ttgaagatcg ggtggaccga gatgaggtcc
       61 atgtgggcgt cgtagtagga gtccatcgtc cccacgtcgc gccagtagcc acggtcgcgt
      121 tcggtcgcgc cgggcacgtc gttgtccttg aagtcgtaga cataggcgtc ggactcgcgg
      181 acgaaccgcg gcacgatgtc gccacccatg tcgtgcttgc tgccctcgag gtcgtggtcc
      241 tcggtgaccg cctggacgag tttgctcgcc gtgaagacgt agttgcccat ggaggcgagg
      301 atctcgtggg gcgcgtcggg gaggcccacc gggtcggtcg gcttctcgcg gaacgccccg
      361 atcttgcggg tgtcagtggg gtcgacctcg atgacgccga actggtcggc catgtggatc
      421 ggctgacgga tggcggcgac ggtcagggcc gcgcccgtct cgatgtgctg ctggaccatc
      481 tgcgagaagt ccatgcggta gacgtggtcg gcaccgacga cgacgacgat ctcgggcttc
      541 tcgtcatcga cgaggttgag gctctggaag atcgcgtctg cgctgccgag ataccagttc
      601 ttgtccacgc gctgctgggc cggcaccggg gcgacgtagt tgccgaggat cgtcgacatg
      661 cgccacgtct tggtgacgtg cttgtccagg ctgtgggact tgtactgcgt caacacgacg
      721 accttgaggt agccggagtt gacgacgttg gacagggcga agtcgatgag tcgatagatg
      781 ccgccgaacg gcactgcggg tttggctcgg tctcgcgtga gcggcatcag ccgcttgccc
      841 tccccacccg ccaggacgat ggccagaacc ttggtgtttg cgcctctgcg tgccatgggt
      901 cccaccctag gggtaggccg ctgcggaaga aacgggtgat ccacgttggg cttgccactg
      961 tctggcgagg gcctcgatca ctacggttga cccatgcgcg tcgacatcat cagcaaggaa
     1021 tatccgcccg ccatctacgg aggcgcgggg gtgcatgtgg ctgagctcgt cagagccctg
     1081 cgcgaccgtg gcgacgtcga cgtccaggtc cgctgtttcg gcgcaccgcg cgacgaagcc
     1141 ggcaccacgg cataccccga tctgcccgaa ttggcctctg ccaacccggc tttgcagacg
     1201 ttcggggtcg acgtcacgat cgcccaggac tgtcagggcg cagacatcgt tcactcgcac
     1261 acgtggtacg ccaacctcgc cgggcacctg ggtggactgc tcgccggagc cccgcacgtc
     1321 gtcaccgcgc acagcctcga gccgatgcgt ccgtggaagg ctgagcagct cggcggcggc
     1381 taccgcgtga gctcgttcgc cgagcgggcg gcctacgaag gggctgcggc ggtcatcgcc
     1441 gtgagcgccg ggatgcgcgc cgacatcttg cgcagctatc cgtcgctcga cccggccaag
     1501 gtgcccgtcg tgcacaacgg gatcgactcg cagatgtggc agccgatcca cgacgccgac
     1561 gtctgccgca agcacggcat ggacccggac cggcccgcgg tgaccttcgt cggccggatc
     1621 acccggcaga agggcctgcc ctatctcatg cgggccgccg cgcggctgcc cgaggaggtc
     1681 caggtcgtcc tgtgcgcagg cgcccccgac acgcccgaga tcaaggccga ggtcgagggg
     1741 ctgctggccg acctgcgcgc ccagcgcgac ggcgtcgtct ggatccctga gatgctgccc
     1801 cgcagtgagg tcatctccct gctcacggcg tcgacggtct tcgcctgccc gtcggtctac
     1861 gagcccctcg gcatcgtcaa cctcgaggcg atggcctgcg agttgcccgt cgtcgcgacc
     1921 gcaaccggcg gcattcccga ggtcgtcgtc cccggcgaga ccggctggct cgtgccgatc
     1981 gagcaggtcc aggacggcac cggcacgccc gtcgacccgg agaagttcgt cggcgacctc
     2041 gccgaggctc tcaccaacgc ggtgagcgac cgcgagcggg ctcggacgat ggggttggcc
     2101 ggccgcaagc gtgcggtcga gcagttctcg tgggagtcga tcggcgacaa gacgatggcc
     2161 gtctatcagg acgtcctcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 cgccgaggcc cctcggcgag ggcttaggcc ggtcgccagc cggccacgct catcgacagg
     2341 acgttcgtcg ctcccacgag ggtggcttcg gcttcggtga gcaggcggcg cagcacggca
     2401 gtccgttccg cagaggtgct cgcgtcgagg gcggcgccgc tctcttcgac gccgagcccg
     2461 gccagggcca acgcacgcac gtcgccggcg cgatcgagca ggtgcaacgg ccgcggggcg
     2521 agaccgggcg gcaggccagg gacgctcgcc cgcggcggtg gcgtcgcttc accaccgcgc
     2581 cacggtatgc cgcccaccgt cgccaaccgg gtcgtcgcct ccaacaccgc ttcggtgagt
     2641 cgtcggctga gatcacccac atccggggcc tcggcgatgt ggcgggggac cggctcggtc
     2701 tcgtatgccg cccacgtcgc cagcacgccg gtgtcgcctt ccgggccgaa gcgggtcagc
     2761 gccggcacga gcgccccacc gaccgtcggc gcgtatgccg cctggccggc gtccgtcgct
     2821 gccgcggtca ccgcaagggg ggcacgcggc atacccgtga gaagtcctgg gcgggggagg
     2881 gcgaggaaga cggccgtctc gccgcgttcg gcccagtcgc gcagggtcgc cttgaagggc
     2941 ccggcgatgt cggtcgcggc gcctgagtga acgttgccga cgacatcgtc gaggtcgcct
     3001 cgaccgccca gccatcgggt gccccagagg gcacaccgga cgacgatcgg gagcgcgagc
     3061 accggcacac cataaccatc ggctcggccg aatgcccgcc tccaccggcg ggtttggacc
     3121 tggggctgaa cctgaatggg ggtgccgaca cgctccctcg gcgcggggcc gcgaactcct
     3181 cgcgggctag ggtgggtgac catgagtgat gtgctcgcct tcgcgggcgt cagcgtggtg
     3241 cgcggccata accgcctcct cgacgacgtc acctgggagg tcgaggaagg cgaacgctgg
     3301 gtgatcctcg gccccaacgg ggccggcaag accactctgt tgcagatcgc cgccgcgcgc
     3361 atgcacccca ccaccggtgt cgtcgggatc ctcggcgagg tgttgggcac cgttgacgtc
     3421 ttcgagttgc ggccgcgggt cgggttggcc agtgcctcga tcgccgagcg gatccccggc
     3481 ggtgagagcg tcggcaacgt cgtcgtcacc gcgtcctacg ggatcatcgg gcgctggcgt
     3541 gaaagctatg accgccagga ctacgcgcgg gcgatggggc tgctcgatgc tctcggcgtc
     3601 gcccatctgg ctgatcggct gtatggcacg ttgtccgagg gcgagcgcaa gcgggtgcag
     3661 atcgcccggg cgctgatgac cgaccctgag ctgatgctgc tggacgagcc ggccgcgggg
     3721 ctggaccttg gcgggcgtga ggatttggtc gcccgccttg gggtgttggc ggaagacacc
     3781 gaggccccgg ccctcgttct cgtcacccac cacgtcgagg agatcccgcc gggcttcacc
     3841 gatgtgctcc tgctgcgcga ggggcggatc gtcgcggcgg gcccgttgcc gctggcgctg
     3901 acctcggcca acctgtcggc gaccttcggt ctgccgttgg tcgtcgagcg gcacggcaag
     3961 cggtggagtg cccgcgcggc cgacccggaa tcgacacctt ccacaggctg acccacccaa
     4021 ggaacctctg tcgtggggta ggcgtcgtag ggtgaaggag tcggtcgtct cggccggctc
     4081 tgcagagagg tgaagcggtg tttcaggggt ctagttggct catctgggtc ggagtggcct
     4141 tggcgctcgg tgcgatcgag gccgcgaccg tcgacttcat cttcctcatg ttggccggcg
     4201 gtgcattggc tggagcagtc gccgccgcct tcggagcgga cttcccgggc caagcgatcg
     4261 tcgcgtcggt ctcggcggtc gcgctcctcg tcggagtccg gccgtgggtc aagcggcgat
     4321 tcaacgtcaa gaccgagcgc cccacggtgg gcatcgccgg aaacgtcggg cgcacggcaa
     4381 ccgtccttga gaccgtgact cttgcgggtg gccggatcaa cctctccggt gagacgtggt
     4441 cggcccgctc ccagggcggg gacgcgatcc tgcccggcga caaggtcgag gtcatcgcca
     4501 tcgacggcgc gacggccgtg gtcgcgcccg tgactctgcc accccagccc ctgccctgat
     4561 cggcgggctc ccactcctgc gcgttcgacc gttcgaccgg cgagtagccg ccaagcccca
     4621 tcggaggaac catgcccaga atcgaaacaa cgatcgtcct gcttctgctg ctcgtgttcg
     4681 caatcactgt cgtcgtccgg accattcgga tcgtgccgca gcagacggcg gtgatcatcg
     4741 agcggctcgg gtcctactcc cgcaccctcg acgacggcat gcacgtcctc gtgccgttca
     4801 tcgacaagcc ccgagcgacc atcgacctgc gtgagcaggt cgtcaccttc cctccgcagc
     4861 cggtcatcac cagcgacaac ctcgtcgtgt ccatcgacac ggtcatctac tacacggtga
     4921 ccgacgccaa gtcggcggtc tacgagatcg ccaacttcat ccagggcatc gagcagctca
     4981 ccgtcaccac
//
LOCUS       DAORPW010000170         4968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4882_length_4968_cov_737.854264, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000170 DAORPW010000000
VERSION     DAORPW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4882_length_4968_cov_737.854264"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /gene="arc"
                     /locus_tag="PLB86_10985"
     CDS             complement(<1..1533)
                     /gene="arc"
                     /locus_tag="PLB86_10985"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HRC65706.1"
                     /translation="MAQEPTPHPATDDHAEAVAAQVAAEITHLQAEVAELRRGRTDPA
                     RVRELEHRIAAQASSMATLSAQNERLVRTLKDARSQIVNLKSEVDRLAQPPNAYGLVL
                     EPAADGVVDILTSGRKMRVAVGAAIESQVLPGREVMLNEALNVVAVKGYEKVGEVVLV
                     KEVLDDDRVLVVAHADEERVCRRAETLRGEHIRVGDALTIDPRSGFVFERVPKAEVQD
                     LVLEEVPDIAYEDIGGLAGQIEAIRDAVELPYLHPELYREHHLKPPKGVLLYGPPGCG
                     KTLIAKAVAASLARKVAEKTGKPVEKSYFLNIKGPELLNKYVGETERHIRLIFQRARE
                     KASDGTPVVVFFDEMDSLFRTRGSGVSSDVETTIVPQLLSEIDGVERLENVIVIGASN
                     REDMIDPAILRPGRLDAKIKIERPDREAARDIFSKYLTVDLPLHEDDVAEHGGDRERC
                     VEAMIQTAVERMYAESDENRFLEVTYAGGDKEILYFKDFNSGAMIQNVVDRAKKNAIK
                     EFL"
     gene            complement(1638..2684)
                     /locus_tag="PLB86_10990"
     CDS             complement(1638..2684)
                     /locus_tag="PLB86_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HRC65707.1"
                     /translation="MTAEPRRGTQAQRGAFVVGDRVQLTDAKGRLHTITLEAGREFHT
                     HQGRLAHDELIGAPEGSVVANSAGVEYLALRPLLADYVMSMPRGAAVIYPKDAGQIVA
                     MGDIHPGARVVEAGVGSGALSMSLLRAVGDEGELISFERRADFAGIAQGNVRAFFGRE
                     HPAWRVVVGDLVESLPGEVEPDSVDRVVLDMLAPWECIDVVATALRPGGVLVCYVATT
                     TQLSRVAEGLREHSGWTEPSAWESLVRGWHLEGLAVRPEHRMHGHTGFLISARRLADG
                     VVTPRRTRRPAKGAYGETGADGIPDGGGEASGSEAVAGVADADWTPEALGERPISDRK
                     VRRAHRTVTGTSAE"
     gene            complement(2696..3040)
                     /locus_tag="PLB86_10995"
     CDS             complement(2696..3040)
                     /locus_tag="PLB86_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRC65708.1"
                     /translation="MAAERAEDPVLNEVHLAGRVGSEPEERELPSGDLVVTLRLVVPR
                     GAARRSPRGPTVDAIDVSCWTAATRRSALRLRPNDQAVVDGSLRRRFFRTGGGVQSRY
                     EVEAVKVRRRAT"
     gene            complement(3212..4345)
                     /locus_tag="PLB86_11000"
     CDS             complement(3212..4345)
                     /locus_tag="PLB86_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRC65709.1"
                     /translation="MSPAADPQYGWLLGRVAGIPVYIGRSWPIIAVVVVMTFGPQLAG
                     ALPGGAGYAVAAAYALLLLVSVLVHEAGHALAARSTGSEVSRIVANLWGGHTVFGSGQ
                     RRPGTAALIAIAGPAGNLLLAGVGWLLLQVDLPAVPALLAWALAFSNVFVAVFNLLPG
                     LPLDGGFVVEAVVWKVTGDRWLAMVVAGWLGRVVTVLAVVYLLAEPLLSGGRPSLVTI
                     VWVALIGAFLWQGATQAIRAGTARRAVARVRVGQVLRPVVVVPGSASAATALEGFARL
                     PGAERAVLVDPMGTPVGFLDLAALSAVPVDQLPVVPATAVLVRPPSGWVVQADPGDDA
                     SLVVSALAGHVEGENVKEAVLVCDSLGQLIGTVSLADVEAALR"
     gene            4523..>4968
                     /locus_tag="PLB86_11005"
     CDS             4523..>4968
                     /locus_tag="PLB86_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRC65710.1"
                     /translation="MPETVSAPVRATEVPSSPRPDATVALTQALRTRILVLDGAMGTL
                     IQRHELTEADYRGARFADWGQDLKGNNDLLTITQPQIIADIHRAYLDAGSDLIETNTF
                     NANRISLADYGMSDLAYELNIAAAALARTEADAATARTPGQPRYVAG"
BASE COUNT          816 a         1863 c         1582 g          707 t
ORIGIN      
        1 gaggaactcc ttgatggcgt tcttcttggc ccggtcgacg acgttctgga tcatcgcgcc
       61 ggagttgaag tccttgaagt agaggatctc cttgtcgccc ccggcgtacg tcacctcgag
      121 gaagcggttc tcgtcgctct cggcatacat ccgctcgact gcggtctgga tcatcgcctc
      181 gacgcagcgc tcccgatcgc caccgtgctc ggcgacgtcg tcctcgtgca ggggcaggtc
      241 gacggtcagg tacttggaga agatgtcgcg cgccgcctca cggtcgggac gctcgatctt
      301 gatcttggcg tccaaccggc ccggacgcag gatcgccggg tcgatcatgt cctcgcggtt
      361 ggaggcaccg atgacgatga cgttctcaag ccgttcgaca ccgtcgatct cggagagcaa
      421 ctgcggcacg atcgtcgtct cgacgtcgct ggacacgccc gacccgcggg tgcggaacag
      481 cgaatccatc tcatcgaaga acaccaccac gggcgtcccg tccgaggcct tctcacgagc
      541 ccgctggaag atcaaccgga tgtgccgctc agtctccccg acgtacttgt tgagcagttc
      601 ggggcccttg atgttgagga agtagctctt ctcaaccggc ttcccggtct tctccgcgac
      661 cttgcgggcc aacgacgccg ccacggcctt ggcgatgagg gtcttcccgc agccgggagg
      721 cccgtaaagc aggacgccct tcggcggctt gaggtggtgc tcgcggtaga gctcggggtg
      781 cagataagga agctcgacgg cgtcacgaat ggcttcgatc tgcccggcca gcccgccgat
      841 gtcctcgtag gcgatgtcgg ggacctcctc cagcacgagg tcctgaacct ccgccttagg
      901 cacccgttcg aagacgaaac ccgacctcgg gtcgatcgtc agcgcgtcac cgacgcggat
      961 gtgttcgccg cgcagcgtct cggcgcgacg gcatacccgc tcctcgtcgg cgtgcgcgac
     1021 gaccaggaca cggtcgtcgt caagcacttc cttgaccagg acgacctcgc cgaccttctc
     1081 gtagcccttg acggcgacga cgttgagggc ctcgttgagc atgacctcgc ggccaggcag
     1141 gacctgcgac tcgatcgccg caccgacggc gacgcgcatc ttgcgaccgg aggtgaggat
     1201 gtcgacgaca ccgtctgcgg ccggctcgag gaccaggcca taggcgttcg gcggttgcgc
     1261 gagccggtcg acctcggact tgaggttgac gatctggctg cgagcgtcct tgagcgtgcg
     1321 cacgaggcgc tcattctggg ccgagagggt ggccatcgag ctggcctgcg cggcgatccg
     1381 gtgttcgagc tcgcggacgc gggccgggtc ggtgcgccct cggcggagct cggccacctc
     1441 ggcctgcagg tgggtgatct cggccgccac ttgggcggca acggcttcgg catggtcgtc
     1501 ggtggccggg tgcggagtgg gctcctgtgc catgacgcct cctgtcctgg gttgggccgt
     1561 cgccggggga acgcggtcgg tcagcggtgc tctcttgaca ctaacccccg actcttgaca
     1621 ttaactcccg ccggccccta ctccgcggac gttccggtca cagttcggtg tgcccgtctc
     1681 accttccggt cgctgatggg gcgttcgccg agcgcttcgg gcgtccagtc ggcgtcagcc
     1741 acgcccgcga ccgcctcaga cccgctcgcc tcgccacccc catcgggtat gccgtccgcc
     1801 cccgtttcgc cgtaggcccc cttcgcgggg cggcgggtcc ggcgcggagt gacgaccccg
     1861 tcggccagcc gccgagccga gatgaggaag ccggtgtggc catgcatccg gtgctccggg
     1921 cggaccgcca gaccctccag atgccagccg cgcacaaggg actcccacgc cgacggctcg
     1981 gtccacccgc tgtgctcgcg cagcccttcg gcaacccggg acaactgggt cgtcgtcgcg
     2041 acgtaacaga cgagcactcc cccaggccgc aacgccgtgg cgacgacgtc gatgcattcc
     2101 cagggggcga gcatgtccag gacgacccgg tccacgctgt caggctccac ctcaccgggc
     2161 aacgactcga ccaggtcgcc gaccaccacg cgccacgccg ggtgctcccg gccgaagaag
     2221 gccctgacat tgccctgggc gatcccggcg aagtcggccc gacgctcgaa ggagatcaac
     2281 tccccctcgt cgcccacggc acggagcagc gacatcgaca gtgccccgga accgacgccg
     2341 gcctcgacga cgcgggcgcc cgggtggatg tcgcccatcg cgacgatctg cccggcgtcc
     2401 ttgggataga tcaccgcagc gccgcgtggc atcgacatga cgtagtcggc cagcagcggt
     2461 cgcagcgcga ggtattcgac ccccgctgag ttggcgacga cgctgccctc gggtgccccg
     2521 atcagctcgt cgtgggccag ccgaccctgg tgggtgtgga actcgcggcc cgcctccagg
     2581 gtgatcgtgt ggaggcgtcc cttggcatcg gtgagttgca cccgatcacc gacgacgaac
     2641 gcccctcgct gggcttgggt gcctcgcctc ggctctgcag tcatggcgag tgagtctagg
     2701 tggcccgccg ccgcaccttc acagcctcca cctcgtatcg gctctgcact cccccgccgg
     2761 tacggaagaa cctccggcgc aacgagccat cgacaacggc ctgatcgttg ggacgtaggc
     2821 gcagcgccga gcgtctcgtg gccgccgtcc agcaggagac gtcgatcgcg tccactgtcg
     2881 ggccgcgcgg tgatcgccgc gcggcccctc gtggcacgac gagccggagc gtcaccacca
     2941 ggtcgccgct gggcaactcc cgctcctccg gctctgaacc cacccgaccg gccaagtgga
     3001 cctcgttgag cacgggatcc tccgcgcgtt ccgccgccat ctcgactcct cccggtgccc
     3061 gcgggcccgt gtcgcgggcg ccgtgtggtc gcgccgacac cgacacctca cacccaccag
     3121 ctgggtgaat ccagcgctcc aggggaacct gtggacagtg ggacgaccga ccacgggcat
     3181 gtggacaacc agagccagcc cccacaaagg ctcagcgcag agcggcctcg acatcggcga
     3241 gtgagacggt cccgatcagc tggcccaacg agtcgcagac cagcacggcc tccttgacat
     3301 tctcaccctc gacatggccg gcgagcgcgg aaacgaccaa agacgcatcg tcaccagggt
     3361 cggcctgcac gacccacccc gagggcggac gaaccaagac cgccgtcgca ggaaccacag
     3421 gcaactgatc gacgggcacc gcactcaagg cagccaggtc caggaacccc acgggcgtcc
     3481 ccatcgggtc gaccaggacg gcgcgctcgg cccccggcag ccgcgcgaac ccttccagcg
     3541 cagtggccgc gctggcagac cccggcacga cgacgaccgg acgcagcacc tgtcccaccc
     3601 gcacccgcgc caccgcccgg cgggccgtcc ccgcgcggat cgcctgggtg gccccctgcc
     3661 agaggaacgc cccgatgagc gcgacccaca caatcgtcac cagggagggt cgaccgcctg
     3721 acagcagggg ctcggcgagg aggtagacga ccgcaagcac cgtgacgacg cggcccaacc
     3781 atcccgcgac gaccatggcc agccaccggt cgccggtgac cttccagacc accgcctcga
     3841 cgacgaaacc accgtccagc ggcagccccg gcagcaggtt gaagaccgca acaaatacgt
     3901 tcgagaaggc caacgcccaa gcgagcagcg cgggcaccgc cggcaggtcg acctgcagca
     3961 ggagccaccc gactcccgca agcagcaggt tccccgcggg cccggcgatc gcgatcaacg
     4021 cggcggttcc gggccgccgt tgccccgacc caaagacggt gtgcccgccc cagaggttcg
     4081 cgacgatccg actgacctcg ctgccggtcg accgagccgc gagcgcgtgg cccgcttcgt
     4141 ggacgagcac cgacaccaac aacagcagcg cgtatgccgc cgcgacggca taccccgcgc
     4201 ctcccggaag cgctccggcc agttgcgggc cgaaggtcat cacgacgacg acggcgatga
     4261 tcggccagga ccgaccgata tagacaggta tgccggccac tcgccccagc aaccagccgt
     4321 actgaggatc tgcggccggg ctcatggtcg caaaccctac gtcaccaccc tttccgctcc
     4381 tgcccgctcc acccgcccca gcaccccagc tcgccccagc gcgccccgaa cgtccgggtc
     4441 gcggtcgatc ccccgcaggc gtccgcgtgc gcggcatact ggtccggccg ccccgcccac
     4501 cccatcaagg acccttgtga ctgtgcccga aaccgtgtca gcgcccgtgc gagcgacgga
     4561 ggtgcccagc agcccccgtc ccgacgcgac tgtcgcactc acgcaggcgc tgcgcacccg
     4621 gatcctcgtc ctggacggcg cgatgggcac cctcatccag cgccacgagt tgaccgaggc
     4681 cgactaccgc ggagcccggt tcgccgactg gggtcaggac ctcaagggca acaacgacct
     4741 gctgacgatc acccagccgc agatcatcgc cgacatccac cgggcctacc tcgatgcagg
     4801 cagcgacctc atcgagacca acacgttcaa cgccaaccgg atctcgctgg ccgactacgg
     4861 catgtccgac ctcgcctacg agctcaacat cgctgcggcg gcgctggccc gcaccgaggc
     4921 cgacgcggcg accgcccgca cccccggcca gccgcgctat gtcgccgg
//
LOCUS       DAORPW010000171         4940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4935_length_4940_cov_603.876970, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000171 DAORPW010000000
VERSION     DAORPW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4935_length_4940_cov_603.876970"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(456..935)
                     /locus_tag="PLB86_11010"
     CDS             complement(456..935)
                     /locus_tag="PLB86_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65711.1"
                     /translation="MASFRDIPGLEPPDAPLWVDHVLRWCVVATFLGYFAFIFRWIDS
                     ATWWGPWLPALPGVLGPLVVLPWWWPPERRPADALRLIVAGLIPAMFPAFALLSLPIA
                     LAGLLIVGLVVLAPRLATEGMRSVGAPSGRIRLWTHSRYLRKSAATSRARRRARGKQ"
     gene            complement(1009..1215)
                     /locus_tag="PLB86_11015"
     CDS             complement(1009..1215)
                     /locus_tag="PLB86_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65712.1"
                     /translation="MATFRGIPGLEPPDALRLIVAGLIPAGAPAFALLSLPIALAVLL
                     SMFTIGRFRRQALANYRASRRERR"
     gene            complement(1305..2708)
                     /locus_tag="PLB86_11020"
     CDS             complement(1305..2708)
                     /locus_tag="PLB86_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65713.1"
                     /translation="MSSIARSTHWPPLCATDPTPGDDEIVRSAGRKFQGVADEIERQI
                     AELGKIADGSSSLQGQYVATLQEACEAVKGDLGKIKRRYREVGGLLVGWAPELVDFQR
                     EAERLLDDAQAAKRGLDGLVTITVPYDQMTDEQKADAQQRQRRASELNGDLNEARRKV
                     HDLQDRRDRAAKRVADKIEEASADDLTDSWWDNVKDWFAAHKELIDGICKVLGVIALV
                     ALVVCLVVPGLNIAAGAALATAATYVGIGATSASLLLHTGQAATGTGTWLDVGLDAFS
                     LATFGAGRFLSVGAREAAGAVRVEAATVAGERAAAPILAQAAAAVDDLAKARAAIPWW
                     KIWQWGKAARLEDAMALVKQAGQTEAAAARVAAEEARLAEQVTIKVRNVLRYGDGDLA
                     KIGMQLREDAKYLKGLTESVQLVDEAVGATGKAAAMFLTSGGTTVVSAGSSFYSPEWW
                     NTMRETFVWEVGSLHRP"
     gene            complement(2705..3019)
                     /locus_tag="PLB86_11025"
     CDS             complement(2705..3019)
                     /locus_tag="PLB86_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65714.1"
                     /translation="MTIEVNYAVLTDSANALRRIAGEFNDAVTIKDHYGKAMGAGSIE
                     RALGTFGGNWDRHAKDLVERLQKTESTFRSVAESFREQDRIGAGSIQETGSAPSGGNT
                     PR"
     gene            complement(3093..3650)
                     /gene="ispF"
                     /locus_tag="PLB86_11030"
     CDS             complement(3093..3650)
                     /gene="ispF"
                     /locus_tag="PLB86_11030"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016996018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRC65715.1"
                     /translation="MSLEPVVPVIPRVGVGVDVHAFAPEGSERELWVAGLHWPGERGL
                     DGHSDADVACHAACDALFTAAGIGDLGTHFGTDRPEYAGASGRALLAEAARLVREAGF
                     EIGNVAVQVIGNRPKIGRHRELAEVALSAACGASVSLSGTSTDGLGLTGRGEGIAAIA
                     TAIVYAVRAPTPPPTSPRSDPPTGI"
     gene            complement(3701..4489)
                     /locus_tag="PLB86_11035"
     CDS             complement(3701..4489)
                     /locus_tag="PLB86_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC65716.1"
                     /translation="MAPVAVITGGGTGIGAATAARLAQDGFDVVVCGRRPGPLQETAA
                     AIRAAYPSVTARAIVLDVTDAASVAAAAAEIGPCDVLVNNAGGAIGSERVEDGKPDEW
                     RAMYESNVIGVLQVTQALLPALRRSPTGGTIVVVSSIAGLLVYEGGGGYTAAKHGATA
                     VVETLRLELAGEPIRVIEIDPGMVWTDGFSLTRFHGDQGKADAVYAGVDRPLVAADVA
                     ECVAFCVRLPQHVNVDRLIVKPVAQAALHKVIRTPIDWDHSATS"
     gene            complement(4501..>4940)
                     /locus_tag="PLB86_11040"
     CDS             complement(4501..>4940)
                     /locus_tag="PLB86_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRC65717.1"
                     /translation="ERGDSVLAGVRWLPEACDVVLVHDAARAFAPSEVFERVAAAVTE
                     AVPAVVPVLPVVDTVKVVDAEGFVVSTPDRAALRIVQTPQGFRRDVLLAAHAAHGSLA
                     TDDAGLVELLGLRVGTVPGDPAAYKITSPADLAHAERLVDHRG"
BASE COUNT          809 a         1796 c         1633 g          702 t
ORIGIN      
        1 tgccgccccg cctcaccgga gcttccgcct gttggccctg tctgcggcca ggatcagcgc
       61 gatgacaccg aggaccccgg cgacgacgca gacccgaaag acatgctcga cccaccgttg
      121 cgcaccgggg ggatctgcac ccggtatgcc gtgaaaggtc gccacggcgt cagcctaacg
      181 cccagcggct cccacccctt ggcgcgcgaa cccgtgccgc ccagcgcctt cctgctgtcg
      241 cctacgcaca acatcgtcaa tgttctgcac actcaccgaa acatcgtcga gcaaacgaaa
      301 gttcgtcgac gttgccgggg ccgagtccga ctacaggtca cgcaccgaga agtcgccgat
      361 gaagtcgccg ttactgctgt cccaacgaca gtcacgacga cttcatgggc gccttcatgc
      421 gcgcagcgaa cgacatcgag gtgctcgagt cccgctcatt gctttccgcg agcgcgccga
      481 cgcgccctgc tggtggcggc cgacttgcgc agatatcggg agtgggtcca gagccggatc
      541 ctcccgctcg gcgccccgac agagcgcatc ccctcggtcg ccaggcgcgg cgcgagcacg
      601 accagcccga cgatgaggag cccggcgagg gcgatcggca gcgacagcag ggcgaatgcg
      661 gggaacatgg ccggaatcaa cccagccacg atcagcctga gcgcgtcagc ggggcgccgc
      721 tccggtggcc accaccacgg caacaccacc agcggcccga ggaccccggg cagcgccggc
      781 aaccaggggc cccaccacgt ggccgagtcg atccagcgga agataaaggc gaagtagccc
      841 aggaaggtcg cgacgacgca ccaccgcagg acatggtcga cccacaacgg ggcatcaggc
      901 ggttcaaggc ccggtatgtc gcggaagctc gccacggact caggctaacg cccggcagct
      961 cccaccacct gatggcaccg gctgggcggg cacagagccg cggcccactc accgacgctc
     1021 tcgccgggaa gcccggtagt tggccagggc ctggcgacga aagcgcccaa tggtgaacat
     1081 ggacaggagc acggcaaggg cgatcggcag cgacagcagg gcgaatgcgg gagccccggc
     1141 cggaatgagc ccggccacga tcagcctcag cgcgtcaggc ggttcaagcc caggtatgcc
     1201 gcggaaggtc gccactcaga cagcctaggc ggatacgtct gcccatcccg agacctgagt
     1261 ctcgatctga ccgtggacgg tgccgttggc ccgcctccga cgccctaggg ccggtggaga
     1321 ctgcccacct cccaaacgaa tgtctctctc atcgtgttcc accactcagg ggagtagaag
     1381 ctactcccgg ccgagaccac ggtggtgcct ccgctggtca ggaacatcgc cgctgcctta
     1441 ccggtcgcac cgaccgcctc gtcaacgagt tgaaccgact ccgtcaggcc cttcaaatac
     1501 tttgcgtcct cgcgaagttg catgccgatc ttcgccaaat ctccgtcccc gtagcgaagg
     1561 acgttgcgga ccttgatagt cacctgctcg gcaagcctgg cctcctcggc ggcgacacgc
     1621 gcggcggcgg cctctgtttg accggcctgc ttcacgaggg ccatcgcgtc ctcaagcctc
     1681 gcagccttgc cccattgcca gatcttccac caagggatcg ccgcgcgagc cttagccaag
     1741 tcgtccaccg cggcagcggc ctgcgcgagg atgggagcag cagcccgctc gccggccacc
     1801 gtcgctgcct cgacccggac cgcgccggcc gcctcgcgag ccccgacgct gaggaaccgg
     1861 cccgcgccga aggttgccag cgagaaggcg tccaggccga catcgagcca cgtccccgtc
     1921 ccggtcgccg cttggccggt atgcaagagc aacgaggccg acgtggcacc gatgccgaca
     1981 taggtcgccg cggtggcgag cgccgcgccc gcggcgatgt tgagccccgg gacaaccaga
     2041 cagaccacga gcgcgaccag cgcgatgacg ccgaggacct tgcagatccc gtcgatgagc
     2101 tctttgtgag cggcaaacca gtccttgacg ttgtcccacc acgagtcggt gaggtcgtcg
     2161 gcgctggctt cctcgatctt gtcggcgacc cgcttggcgg cgcgatctcg gcggtcctgc
     2221 aggtcgtgca ccttgcgccg ggcctcgttg aggtcgccgt tcaactcgct cgcccgacgt
     2281 tggcgttgct gcgcgtcggc cttctgctcg tcggtcattt ggtcataggg gaccgtgatg
     2341 gtgacgagcc cgtcgagccc ccgtttggcc gcctgagcgt cgtcgagcaa ccgctcggcc
     2401 tcgcgctgga agtccaccag ctctggcgcc caaccgacga gcagcccacc cacctcgcga
     2461 tagcgacgct tgatcttgcc cagatcgccc ttcacggcct cgcaggcctc ctgcagggtg
     2521 gcgacatact gcccctgcaa ggacgacgag ccatccgcga tcttgccgag ctcagcgatc
     2581 tggcgctcga tctcgtcggc gacaccctgg aacttgcgcc ccgccgaccg gacgatttcg
     2641 tcgtcgcccg gcgtcgggtc agtggcgcac agtggtggcc agtgtgtcga tctcgcgatg
     2701 ctgctcatcg aggagtgttc cccccgctgg gggccgagcc ggtctcctgg atcgagccgg
     2761 cgccaatccg atcctgctca cggaacgact cagcgaccga acggaaggtc gactcggtct
     2821 tctgcagacg ctcgaccagg tccttcgcgt gccggtccca gttgcccccg aaggtcccga
     2881 gcgcccgctc gatcgagccc gcccccatgg ccttgccgta gtggtccttg atggtcacgg
     2941 cgtcgttgaa ctcaccggcg atccgccgca gcgcgttggc gctgtcggtg agcacggcgt
     3001 agttgacctc gatcgtcacc agagaccttt ctgtctttcc ttcgtccgga gtgtttcacc
     3061 gccgccagcg gctcaccagt ccgtactcgt actcagatgc cggtgggcgg gtccgagcgc
     3121 ggggacgtcg gcggaggcgt cggcgcgcgc acggcataca cgatggcggt ggcgatggcg
     3181 gcgatcccct cgccgcgacc ggtgagcccg aggccgtcgg tcgacgtccc cgacaacgac
     3241 accgaggcgc cgcaggcggc cgacagggca acctcggcga gctcgcggtg ccgaccgatc
     3301 ttcgggcggt tgccgatcac ctgcacggcg acgttgccga tctcgaagcc cgcctcgcgg
     3361 acgaggcgcg cagcctcggc gagcagggcg cgcccggacg cgccggcata ctccgggcgg
     3421 tcggtgccga aatgggtgcc gaggtcgcca atcccggccg cggtgaagag cgcgtcgcag
     3481 gcggcgtggc aggcgacgtc ggcatccgag tgcccgtcca acccgcgctc ccctggccag
     3541 tgcaggccgg caacccagag ctcacgctcc gagccctccg gtgcaaaggc gtggacgtcc
     3601 accccgaccc ccacccgcgg aatgaccggc acgaccggct caaggctcat cgcccgagcg
     3661 tcccacacca gggcgcgcgc cggagcacgg accacgcgcg tcaggaggtt gcgctgtggt
     3721 cccagtcgat cggcgtgcgg atcaccttgt gcaaagccgc ctgagcgacc ggcttgacga
     3781 tgagccggtc gacgttgacg tgctgcggca gccggacgca aaacgcgacg cactcggcga
     3841 catcggcggc cacgagcgga cggtcgacac cggcatacac cgcgtcggcc ttgccctggt
     3901 cgccgtggaa gcgggtgagc gagaagccgt cggtccacac catgcccggg tcgatctcga
     3961 tcacccggat cggctcaccg gcgagttcga ggcgcagcgt ctcgacgact gccgtcgcgc
     4021 cgtgcttggc cgccgtgtag cccccgccgc cctcgtaaac cagcagtccc gcgatggacg
     4081 agacgacgac gatggtgccg ccggtcggtg agcgtcgcag ggccggcaac agcgcctggg
     4141 tgacctgcaa gacgccgatg acgttcgatt cgtacatcgc gcgccactcg tcaggcttgc
     4201 cgtcctcgac ccgctcgctg ccgatcgcgc cgcccgcgtt gttgaccagg acgtcgcacg
     4261 gcccgatctc cgcggcagcc gcagccacgc tcgccgcgtc agtcacgtcc aggacgatcg
     4321 cgcgggcggt caccgagggg tatgccgcgc ggatcgcggc cgcggtctcc tgcagcggcc
     4381 cggggcggcg gccacagacg acgacatcga agccgtcctg ggccaggcga gcggcagtgg
     4441 cagcaccgat cccagtgccc cctccggtga taacagcaac gggtgccatg gcgggtcctt
     4501 tcaccctcgg tggtcgacga ggcgctcggc gtgggcgagg tctgcgggcg aggtgatctt
     4561 gtatgccgcg gggtcacctg ggacggtgcc gacgcgcagg ccaagcaact cgacgagccc
     4621 ggcatcgtcg gtggccaggc tgccatgcgc cgcgtgggcg gcgagcaaca catcgcggcg
     4681 gaagccctgc ggagtttgga cgatccgcag ggcggcccgg tccggagtcg acaccacgaa
     4741 accctcggcg tccacgacct tgacggtgtc gaccaccggc agcaccggca cgacggccgg
     4801 gaccgcctcg gtcaccgcgg cggcgacccg ctcgaagacc tcgctcgggg cgaacgcccg
     4861 cgccgcgtcg tgcacgagga cgacgtcgca cgcctcgggc agccaccgca ccccggccaa
     4921 aacagagtcg ccgcgctcgg
//
LOCUS       DAORPW010000172         4934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4944_length_4934_cov_561.912687, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000172 DAORPW010000000
VERSION     DAORPW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4944_length_4934_cov_561.912687"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..652)
                     /locus_tag="PLB86_11045"
     CDS             complement(83..652)
                     /locus_tag="PLB86_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRC65718.1"
                     /translation="MSRREELLGQVVAWFAAHGIGDTSLRTLARELGTSHRMLIYHFG
                     SREGLLTEVVGAVEAGERAALAALLEVAHEDPYAAGAAFWTHVADAATTFAPLFFEVS
                     GLAMQGASYAAGLRSWLADGWLDGLAAGFEAGGFTPSRSRELAQESLAMARGLLFELA
                     VSGDRQGVDAAMGRYVEEVRFRGNSLGCI"
     gene            complement(649..1104)
                     /locus_tag="PLB86_11050"
     CDS             complement(649..1104)
                     /locus_tag="PLB86_11050"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC65719.1"
                     /translation="MSISVTSSTIVNPSLAWQVLSDVRSWPEWLPTVIEVVPETPEAP
                     DGMGAAYLVRQPRLAAARWEITEWRPGAGFTWVSRRPGVTTTGTHQLVEVPGGVQAQL
                     GIAWSGPLAWLVRASYGGLTRGYVASEAAAIVAQCQVQQSEGETAQTSQ"
     gene            complement(1146..2951)
                     /locus_tag="PLB86_11055"
     CDS             complement(1146..2951)
                     /locus_tag="PLB86_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65720.1"
                     /translation="MSRFEPLTDHSGISSGGELSAWATIRRGAAISPELTKGLAVTVL
                     LAGLSTVGRVLIPFVVQRTTDEGILAPGGPDASRVLLWVALALAGVVVTAVSSYAVNV
                     RLYTASESGLASLRLAAFRHIHDLSMLTQNTERRGSLVSRVTSDVDTISAFVQFGGLM
                     LIVSTGQILLATVLMVVYSPVLAAVVWGCFVPLFFLIRHFQGVVGRAYTRVRQRVGEM
                     LGAISEAVVGAATIRAYGIEERTAARIDAAIEAHRREAVGAQVRSVIAFTAGQFVSGL
                     TSAVVLVIGTVLAVRGDVTIGTLLAFLFLVNLFTMPVQQATEILNEMQNAVAGWRRVI
                     GIIDTPADVADPGPGGVELPRGPIIVRFDDVSYAYPGGGPVLREVTLDIAPRARIAVV
                     GETGSGKSTLAKLLTRLMDPTEGQVLVDGVDLRRVRFSSLRSRVVLVPQEGFLFDASL
                     LENIRFGRPEASVDDVHLALTELGLDEWAEGLPLGLDTAVGQRGESLSAGERQLVAIV
                     RAYLADPDLLVLDEATSAVDPSTEVRVQRALDSLTRGRTSIAIAHRLSTAEAADEVIV
                     VDAGRIVERGPHVELVNAGGVYAALHASWAAQQRA"
     gene            complement(2948..4807)
                     /locus_tag="PLB86_11060"
     CDS             complement(2948..4807)
                     /locus_tag="PLB86_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65721.1"
                     /translation="MTTAPIPAPAVPAATGTTLRRGLAVIARGVREEPRWFSLAVLGS
                     TIYGIMTGLMAWAIGWCTSTIVTPAVAARTVTSGQLWTIAGVISGVVTVNVLGIILRR
                     TAAGFAMYSLGAAYRRRVSRQYLDLPLRWHHKHPSGQLLSNANADVEATWNIFAPLPM
                     ALGVIVMLVFGAVQMLIVDPVLAAVGMVVFPLLFLANAVFQRRMSPLITRAQQLRAEV
                     SEVAHESFEAALVVKSMGREDQEARRFAEAAYTLRDANILVGRTRGTFDPAIESIPTI
                     GTLAVIAVGAWRAGQGAVTAAEVVQIAYLFSVLAFPVRAFGWVLGELPRSVVGWERVN
                     AVLQATGAMAYGPAQLPDRDAATVAARRLDFAYEIDLPEGGTELSPAIRDVTITVPAG
                     ATIALVGPTGSGKTTLTNLLLRLVDPDSGQVLVDEVDLRTVRQGGVSRVAALVPQQTF
                     MFEDTVRDNVTLGADHTDDEVWEALRVAQARRFVRSLPDELDTRVGERGTNLSGGQRQ
                     RIALARAVIRRPQLLVLDDATSAVDPSVEQEILTALRGVSSATTVLLVAYRMATIALA
                     DEIVYIEEGQVRGHGSHEALVTTCPGYAALVSAYAREAADRAAVAADEEEVAS"
BASE COUNT          825 a         1695 c         1749 g          665 t
ORIGIN      
        1 cggggggacc tgagggctgg ttccaacgtt cgcagtgttg cgccgaatcg cgcgtcgcgt
       61 caatggcccg accggtcaga aattagatgc atcccaacga attcccccgg aatcggactt
      121 cttcgacata tcgacccatg gccgcgtcga ccccctggcg atcaccgctg accgccagtt
      181 cgaacagcag accgcgagcc atcgccaacg actcctgggc caattcacgc gaccgcgacg
      241 gggtgaaacc gcctgcctcg aaccccgccg ccaagccgtc caaccagccg tcggcgagcc
      301 aggatcgcag ccccgcggca tacgaagcac cttgcatggc caagccgctg acctcgaaga
      361 agagcggcgc gaacgtggtg gccgcgtcgg cgacgtgggt ccagaatgcg gcgccggcgg
      421 catacgggtc ctcgtgcgcc acctcgagca gggcggcaag cgctgcccgc tcaccggctt
      481 cgacggcgcc gacgacttcg gtgagcagac cctcgcggga cccgaagtga tagatgagca
      541 tccgatggct cgtgccgagt tctcgggcga gggtgcgcag gctggtgtcg ccgatcccgt
      601 gcgccgcgaa ccacgccacc acctgaccca ggagttcctc ccggcggctc actgcgaggt
      661 ctgcgccgtc tcgccctcgg actgctggac ctggcactgc gcgacgatcg cggccgcctc
      721 gctcgcgacg tacccccgcg tcagcccgcc atacgacgcc cgaacgagcc aggccagcgg
      781 cccgctccag gcgatgccga gctgggcctg gaccccgccg gggacctcga cgagttggtg
      841 ggtgccggtg gtcgtcacgc cgggccgccg cgacacccag gtgaagcctg ccccgggacg
      901 ccactcggtg atctcccaac gcgccgccgc gagccgcggc tgccggacga ggtatgccgc
      961 ccccatcccg tcgggtgcct cgggggtctc ggggacgacc tcgatgacgg tcgggagcca
     1021 ctcgggccag cttcgaacgt ccgacaggac ctgccaagcc aggctcggat tgacgatggt
     1081 tgacgacgtg acggaaatag acatgtacca gatggtacaa gtgcgatcgg gcgctcgcag
     1141 ggctttcagg ctcgttgttg ggccgcccag gaggcgtgca gcgcggcata cacgccgccg
     1201 gcgttgacca actctacgtg cgggccgcgt tcgacgatcc gacccgcgtc gacgacgatc
     1261 acctcgtccg cggcttcggc cgtggagagc cggtgagcga tggcgatcga cgtccggccc
     1321 cgggtgaggg agtccaaggc ccgctggact cgcacctcgg tcgaggggtc gacggcagac
     1381 gtggcctcat cgaggacgag caggtcggga tcggcgagat aggcccggac gatggcgacg
     1441 agttggcgct cccccgccga caacgactcg ccgcgctggc cgaccgccgt gtcgaggccg
     1501 agtggcaggc cttctgccca ctcgtccagc cccagctcgg tgagcgccag gtgcacgtcg
     1561 tcgaccgagg cttccggtcg tccgaagcgg atgttctcga gcaacgaggc gtcgaagagg
     1621 aagccctcct gcgggaccag caccacgcga gaacgcaacg aggagaagcg aactcgtcgc
     1681 aggtccactc cgtcgaccag tacctgaccc tcggtcgggt ccatgagtcg ggtgagcagt
     1741 ttggccagcg ttgacttgcc cgaaccggtc tcccccacca cggcgatccg ggctcgcggg
     1801 gcgatgtcga gggtgacctc ccgcaggacc ggcccgccgc cggggtaggc ataggagacg
     1861 tcgtcgaagc gcacgatgat cggcccgcga ggcaactcca ccccgccggg gccggggtcg
     1921 gccacgtcgg ccggagtgtc gatgatgccg atgactcggc gccagccggc gacagcgttc
     1981 tgcatctcgt tgaggatctc ggtggcctgc tgcactggca tggtgaagag gttgacaagg
     2041 aagaggaagg ccagcagggt cccgatcgtc acgtcgccgc ggacggccag aactgtgccg
     2101 atgacgagga cgaccgccga ggtcagcccg gacacgaact ggcccgcggt gaaggcgatg
     2161 acggagcgca cctgggcgcc gaccgcctcg cgccgatggg cctcgatcgc agcgtcgatg
     2221 cgagcagcgg tgcgctcctc gatgccgtag gcgcgaatgg tcgcagcccc gacgacggcc
     2281 tcggagatcg ccccgagcat ctccccgacc cgttggcgca cccgggtgta ggccctcccg
     2341 acaacgccct ggaagtgtcg gatgaggaag aacagcggga cgaaacagcc ccaaaccacg
     2401 gccgcgagca cggggctgta gaccaccatg agcacggtgg cgagcaggat ctggccggtg
     2461 ctgacgatga gcatcagccc gccgaactgg acgaacgccg agatggtgtc cacgtcggag
     2521 gtcacccgcg agacgaggga accccgccgc tcggtgttct gggtgagcat cgacaggtcg
     2581 tggatgtgtc ggaacgcagc caaccgcagg gaggccagcc cgctctcgga cgcggtgtag
     2641 aggcgcacgt tgacggcata cgaactgacg gcggtgacca cgacccccgc cagcgcgagg
     2701 gcgacccaga gcagcacccg gctcgcgtcg ggaccgccgg gcgcgaggat cccttcgtcg
     2761 gtggtgcgct gcacgacgaa ggggatgagc accctcccga ccgtcgagag accggcgagc
     2821 aacacggtga cggccaggcc cttggtgagc tccggagaga tggccgcgcc acgtcggatg
     2881 gtggcccagg cgctcagctc accgcccgaa ctgatccctg agtggtcggt caacggctcg
     2941 aagcggctca cgaggccacc tcttcctcgt cggccgcgac ggccgcccgg tcggccgcct
     3001 cgcgggcata ggccgagacg agcgcggcat acccagggca ggtggtgacc agggcctcgt
     3061 ggctgccatg cccgcggacc tggccctcct cgatgtagac gatctcgtcg gccagggcga
     3121 tagtcgccat ccggtaggcg acgagcagca ccgtcgtcgc gctggagacc ccgcgcaggg
     3181 cggtgaggat ctcctgctcg acactcgggt cgacagccga agtcgcgtcg tcgagcacga
     3241 gcagctgggg acggcgaatc acggctcgcg ccaaggcgat tcgttgacgc tgaccgcctg
     3301 agaggttggt gccccgctcc cccacccgag tgtccagctc atccggcagg gagcggacga
     3361 agcgccgcgc ctgggcgacc cgaagggcct cccacacctc gtcgtcggtg tggtccgcgc
     3421 cgagcgtcac gttgtcgcga acggtgtcct cgaacatgaa ggtctgctgc ggcacgaggg
     3481 cggccacccg agagacgccg ccctggcgca ccgtgcgcag gtcgacctcg tcgacgagta
     3541 cctgaccgga gtcggggtcg acgaggcgca gtaggaggtt ggtcagggtc gtcttgcccg
     3601 agccggtggg gccgacgagg gcgatggtcg cgccggcggg caccgtgatg gtgacgtcgc
     3661 ggatcgccgg ggagagctcg gtgcccccct cgggcaggtc gatctcgtag gcgaagtcca
     3721 gccggcgcgc tgcgaccgtg gccgcgtcac ggtccggcaa ctgggccggg ccgtaggcca
     3781 tcgcgccggt ggcctgcagg acggcattga cccgctccca gccgacgacc gaccggggca
     3841 actcgccgag cacccagccg aaggcgcgga ccgggaaggc caacaccgag aacaggtagg
     3901 cgatctggac cacttcggcg gccgtcaccg ccccctgtcc ggcccgccag gccccgacgg
     3961 cgatgaccgc gagggtgccg atcgtcggga tcgactcgat cgccgggtcg aaggtgccgc
     4021 gggtgcgccc cacgaggatg ttggcatcgc gcaaggtgta tgccgcctcg gcgaaccgcc
     4081 gcgcctcctg gtcttcgcgg cccatcgact tgacgaccag ggcggcctcg aagctctcgt
     4141 gggcgacctc ggacacctcg gcccgcaact gttgggcgcg ggtgatcaac ggcgacatcc
     4201 ggcgttggaa gactgcgttg gcgaggaaga gcagcgggaa gacgaccatc ccgaccgcgg
     4261 cgaggaccgg gtcgacgatg agcatctgga ccgcgccgaa gacgagcatg acgatgaccc
     4321 cgagggccat cggcagcggc gcgaagatgt tccacgtcgc ctcgacgtcg gcgttcgcgt
     4381 tggacagcaa ctgtccggac gggtgcttgt ggtgccagcg cagtggcagg tcgagatact
     4441 gccgggagac cctgcgccgg tatgccgcgc ccaggctgta catcgcgaag cccgccgcgg
     4501 tgcggcgcag gatgatgccc aggacgttga ccgtgacgac gccggagatg accccggcga
     4561 tcgtccagag ttggccggag gtcaccgtgc gggccgcgac tgccggggtg acgatggtgc
     4621 tcgtgcacca accgatggcc caggccatga ggccagtcat gatgccgtag atcgtcgagc
     4681 ccaacaccgc gagcgagaac cagcggggtt cctcgcggac acctcgggcg atgaccgcca
     4741 gacctcgccg gagcgtcgtg ccggtcgctg caggaactgc cggcgccggg atcggcgcag
     4801 tcgtcatgag ggagtccttc gggtgctcgg ggttgcctcc agcctctcat gctgcgccga
     4861 cacccacggt cgctcgcgcc tgactgccgg tcagccccgc tcgcgaacca tcaccgccgg
     4921 ggcgagggcg tcac
//
LOCUS       DAORPW010000173         4933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4945_length_4933_cov_718.854859, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000173 DAORPW010000000
VERSION     DAORPW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4945_length_4933_cov_718.854859"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..737
                     /locus_tag="PLB86_11065"
     CDS             3..737
                     /locus_tag="PLB86_11065"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HRC65722.1"
                     /translation="MAYTLILLRHGHSEWNAKNLFTGWVDVDLNDQGREEAVRGGRLL
                     KDKGVLPDIVHTSVLRRAITTANLALDAADRHWIPVRRDWRLNERHYGALQGLDKAAT
                     RAKYGDEQFMLWRRSFDTPPPPIDLDNEWAQTGDPRYAGIDIPRTECLKDVIARMMPY
                     WESAICPDLASGKTVLVTAHGNSLRALVKHLDGISDADIAELNIPTGQPLVYDIGEDF
                     MPTGPGQYLDPEAAAAAAAAVANQGR"
     gene            complement(758..2062)
                     /locus_tag="PLB86_11070"
     CDS             complement(758..2062)
                     /locus_tag="PLB86_11070"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HRC65723.1"
                     /translation="MPTSKPPQSLFVWTWLPGDTTPVVAGRLIERSGRVDFVYGESYL
                     RRDNAISLYRPELPLGPGLIAPLPGLDLAGSIRDGSPDAWGRRVIDSRLGVGDVDLTE
                     LSYLIASGSNRLGALDFQTSPTRYVPRDETATLDELHEAAIMVEEGRALPQPLAEALI
                     RGTSIGGARPKALLVDGDGTQWIAKFSSSSDTHFSVPNAEGATMILARHAGIDVCDVR
                     VTSSLGREVLLVRRFDRTASGGRIHAVSALTMSGESEYGARYVTYPQILNVLTRLSAS
                     RMPVGPALFRRIVFNVAVSNSDDHARNHAALWDGEHLRLSPAYDLCPGPRSGQTAEQA
                     MAFGRSGIRTSTFHAAISVAAEYGLSTSDARSVVDDVVSAITDHYAEAADQARLSEAD
                     RTAMLGRQFLNPGVWHGLPTTFALPASTAASPSPDQGSGEGD"
     gene            complement(2049..2393)
                     /locus_tag="PLB86_11075"
     CDS             complement(2049..2393)
                     /locus_tag="PLB86_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC65724.1"
                     /translation="MAKRAIVAQPEVLDAARTLGVQIRIARHERGWTAADLASKIGVS
                     AKTVLAIEAGAVSTSIGTVFNAAVMVGVPLFGVEDRFEMARMRRRGEERLALIGQRVR
                     VPKLDETDLANF"
     gene            complement(2503..3216)
                     /gene="phoU"
                     /locus_tag="PLB86_11080"
     CDS             complement(2503..3216)
                     /gene="phoU"
                     /locus_tag="PLB86_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRC65725.1"
                     /translation="MRDAFHEQLDSITAQLVDMTRLAGSAMARATTALLDADLHLAED
                     VITGDRTLDNAREHLDSLAIDVLARQQPVATDLRTVVTALRMSGDLERMGDLARHVAK
                     VARLRFPNSAVPASFRAHILQMGQVAELVVTKSGSIIAARDVTEARNLERDDDVMDEL
                     HRRLFTVLATTELPTETIVDLTLLGRYYERFADHAVSVARRVVYLVTGEYVPETSGDH
                     DVSEEGARIALLRRSSPRG"
     gene            3409..4734
                     /locus_tag="PLB86_11085"
     CDS             3409..4734
                     /locus_tag="PLB86_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC65726.1"
                     /translation="MEVLLAVILGAVFGAVVVSARFGRTRVDRRRALAAGATPDPEDV
                     VEQLPAEVTDLLAALTSSGIVLDSRQSVVACSPTAISHGLVRGTAVSHPPLLALARRA
                     VIEGGVVEEMLGLPRGPRADARRVVQARATHLGLEHILLLVEDRSHAYLVEEVRRDFL
                     ANVSHELKTPVGGLMLLAEAVRDARDDPEAVARFAKRMRKETRRLDQLVSDIVQLSRL
                     QSEDRLSDPDAVDLGGVVRQAVDDVRITAEERRIDLVVRCDANCWVWGDAGLLTTAVR
                     NLVVNAVAYSASGTRVAVRALIAPDGAREIIVSDQGQGIPESELGRIFERFYRIDAAR
                     SRATGGTGLGLSIVKHVCANHGGDVTVWSEVGRGSTFTIRLPAPAYGPDDQSTTGSIG
                     VLAPGDVRGGEGGTAASTADPASPDHHLDGALASTGPQSSDVTREVRPA"
     gene            4731..>4933
                     /locus_tag="PLB86_11090"
     CDS             4731..>4933
                     /locus_tag="PLB86_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC65727.1"
                     /translation="MSRILIVEDEESFSDPLSYLLEKEGFAVAVAATGPDALAEFDAN
                     GADLVLLDLMLPGLSGVEVCRALR"
BASE COUNT          776 a         1713 c         1689 g          755 t
ORIGIN      
        1 gcatggcata caccctcatc ctgctgcgtc acggccactc cgagtggaac gccaagaacc
       61 tcttcaccgg gtgggtcgac gtcgacctca acgaccaggg ccgcgaggaa gccgtccgcg
      121 gcgggcggct gctcaaggac aagggcgtcc tgcccgacat cgtccacacg tccgtgctcc
      181 gccgggcgat caccactgcc aacctggcgc tcgacgccgc cgaccggcac tggatcccgg
      241 tgcgccggga ctggcggctc aacgagcggc actacggcgc gttgcagggc ctggacaagg
      301 ccgcgacccg cgcgaagtac ggcgacgagc agttcatgtt gtggcgccgc tccttcgaca
      361 ccccgccgcc gcccatcgac ctggacaacg agtgggccca gaccggcgac ccccggtatg
      421 ccggcatcga catcccgcgc accgagtgcc tcaaggacgt catcgcccgg atgatgccct
      481 actgggagag cgcgatctgc cccgacctgg cttccggcaa gacggtgctc gtcaccgccc
      541 acggcaacag cctgcgggcc ctggtcaagc atctcgacgg gatcagcgac gcggacatcg
      601 ccgagctcaa catcccgacc ggccagccgt tggtctacga catcggcgag gacttcatgc
      661 cgaccgggcc ggggcagtac ctcgaccccg aggccgctgc tgccgctgct gcggcggtcg
      721 ccaaccaggg ccgctgagcc gacgcggatc gagccagtca gtcgccctcg ccggaccctt
      781 ggtctggcga gggcgacgcg gctgttgagg ccggcaacgc gaaggtggtc ggcaatccgt
      841 gccagacgcc agggttgagg aactgcctcc caagcatggc cgtccggtcg gcttcgctca
      901 gccgggcctg gtcagcggcc tcggcgtagt ggtcggtgat cgctgacacc acgtcgtcca
      961 ccacggatcg ggcgtcgctg gtgctcaggc catattcggc ggccacgctg atggcggcgt
     1021 ggaaggtgga cgtgcggatc ccgctgcgtc cgaacgccat cgcctgctca gcggtctgtc
     1081 cggagcgcgg ccctgggcag aggtcatacg ccggtgagag gcgaaggtgt tcgccgtccc
     1141 acagcgccgc gtggttgcgc gcgtggtcgt cggagttgct gacggcgacg ttgaagacga
     1201 tccgtcggaa gagggctggc ccgaccggca tacggctggc ggaaagccgc gtgaggacgt
     1261 tgaggatctg cggataggtg acatagcggg cgccatactc gctctcaccc gacatggtga
     1321 gggcggacac cgcgtggatc cggccgccgg aagcggtgcg gtcgaaccgc cggaccagaa
     1381 ggacctcgcg ccctaacgac gaggtcaccc gcacatcaca gacgtcgatc ccggcgtgcc
     1441 gcgccaggat catcgtggcc ccttcggcgt ttgggacgct gaagtgggtg tctgacgacg
     1501 atgagaactt ggcgatccac tgggtgccgt cgccgtcgac cagcaacgcc ttcggccggg
     1561 caccgccgat cgacgtgccg cggatcagtg cctcagccag cggctggggg agagcgcgcc
     1621 cttcctccac catgatcgcc gcctcgtgca gctcgtccaa ggtcgcggtc tcgtctcgcg
     1681 ggacataccg ggtcgggctg gtctggaagt ccagcgcccc taacctgttc gagccggatg
     1741 cgatgaggta gctcaactcg gtcaggtcga catccccaac acccaggcgc gagtcgatga
     1801 cgcgccggcc ccaggcgtcg ggggatccgt cacggatgct ccccgccagg tcgagcccag
     1861 ggagcggtgc gatcaggccg gggccgagcg gcaactccgg gcggtagagg ctgatcgcgt
     1921 tgtcgcggcg caagtaggac tcgccataga cgaagtcgac cctgccggag cgctcgatca
     1981 ggcgcccggc gacgacggga gtggtgtcgc cgggcagcca ggtccagacg aacagcgact
     2041 gtgggggctt agaagttggc aaggtcggtc tcgtccagct tcggcacccg gacccgctgg
     2101 ccgatcaggg ccagacgttc ctctccgcgt cggcgcatcc gagccatctc gaaccggtcc
     2161 tcgaccccga acagcggcac gccgaccatg acggccgcgt tgaagaccgt gccgatcgaa
     2221 gtggacacag ccccggcttc gatcgcgaga acggttttgg ccgagacgcc gatcttgctg
     2281 gcgaggtcgg cggcagtcca cccgcgctcg tgccgcgcga tgcggatctg cacgcccagc
     2341 gtccgagcag cgtcgagcac ctcgggttgc gcaacgatgg cgcgcttggc cataggtcct
     2401 ccaataaagc accctttaac actcgtaagg tgcagtatat aactcctgcc cggcaggagt
     2461 cagcggtcaa gcccgatacg gcaaggaggg cgtgagcact cgtcagcccc gcgggctgct
     2521 gcggcgaagg agcgcgatcc gcgcgccctc ctcggagacg tcgtgatccc ctgacgtctc
     2581 tgggacgtat tcgcccgtga cgaggtagac gacgcgacgc gccaccgaca cggcatggtc
     2641 ggcgaaccgc tcgtagtagc gacccaacag agtgaggtcg acgatcgtct cggtcggcaa
     2701 ctcggtggtc gcgagcacgg tgaacaaccg gcggtgcaac tcgtccatga cgtcgtcgtc
     2761 gcgctcaaga ttgcgcgctt cggtcacgtc gcgagcagcg atgatcgagc ccgacttggt
     2821 gaccaccaac tcggcgacct ggcccatctg caggatgtgc gcgcggaacg acgcggggac
     2881 ggccgagttc gggaagcgca gtcgggcgac cttggccacg tgtcgggcga ggtcccccat
     2941 ccgctcgagg tccccactca tccgcagcgc cgtgacgacg gtgcgcaggt cggtggcgac
     3001 cggttgctga cgggccagga cgtcgatggc caggctgtcc aggtgctccc gggcgttgtc
     3061 caacgtgcgg tcgccggtga tgacgtcttc agccagatga aggtcggcgt ccaacagcgc
     3121 ggtggtggct cgcgccatcg ccgaaccagc caggcgggtc atgtcgacca gttgggccgt
     3181 gatgctgtcc aactgctcgt ggaaggcgtc gcgcacgtct gctccttcgc ggatccgggt
     3241 gggaagcggg ggtcgtggct gggcgcggcc acggtctaca gcatcggccc ggccacggtg
     3301 aacggatatc ggcgcccagg tgaacatcgt gcgatgcagg ggcccgcaaa ggcgccggac
     3361 acgccccacc cgaacctcgg catcgtgggt gccctctagc ctaggggcgt ggaggtcctt
     3421 ctcgcggtga tcctcggcgc tgtgttcggc gccgtcgtgg tctccgcgcg gttcggacgg
     3481 acccgtgtcg accgtcgtcg agcgttggcc gcaggtgcca cacccgaccc tgaggatgtc
     3541 gtcgaacagt tgcccgcgga ggtcaccgac ctgctcgccg cgctcacgtc cagcggcatc
     3601 gtcctggatt cccggcagtc ggtcgtcgcc tgttctccca ccgcgatctc gcacggtctg
     3661 gtccgcggca ccgccgtctc gcacccaccc ctgctggccc tggcccgacg cgccgtcatc
     3721 gagggcggcg tcgtcgaaga gatgctcggg ctgccgcgcg gcccccgggc cgacgcccga
     3781 cgggttgttc aggcgagagc gacccacctg ggcttggagc acatcctctt gctcgtcgag
     3841 gaccgctcgc acgcctacct cgtcgaagag gtgcgccgcg acttcctcgc caacgtgagc
     3901 cacgagctca agacccctgt tggtggcctc atgctcctgg ccgaggccgt gcgcgacgcg
     3961 cgtgacgacc cggaggccgt cgcccgcttc gccaagcgga tgcgcaagga gacccgtcgc
     4021 ctggaccaac tcgtcagcga catcgtccag ctctcccgcc tgcagtctga ggaccggctg
     4081 tccgacccgg atgccgtcga cctggggggc gtcgtccgcc aggccgtcga cgacgtccgg
     4141 atcaccgccg aggagcgccg catcgacctc gtcgtgcgct gcgacgccaa ctgctgggtc
     4201 tggggcgacg cgggcctgct gaccaccgct gtgcgcaacc tcgtcgtcaa cgcggtcgcc
     4261 tactccgcct ccggcactag ggtcgccgtg cgggccttga tcgcccctga cggtgcccgc
     4321 gagatcatcg tctccgacca gggacaaggc atccctgaga gcgagttagg gcgcatcttc
     4381 gagcggttct atcggatcga cgcggctcgt tcgcgcgcga ccggaggcac ggggttgggc
     4441 ctgtcgatcg tcaagcacgt gtgcgccaac cacggcggcg acgtcaccgt gtggagcgaa
     4501 gtcggccggg gatcgacgtt cacgatccgg ctgcctgcac cggcatacgg acctgacgac
     4561 cagtccacga ccggatccat cggcgtgctc gccccaggcg atgtgcgcgg gggcgagggt
     4621 ggcaccgccg ccagcaccgc cgacccagct tcacccgatc accacctcga cggggcgttg
     4681 gcctcgacgg gtccacagag cagcgacgtc accagagagg tgcgacccgc atgagccgaa
     4741 tcctcatcgt ggaagacgag gagtccttct ccgacccgtt gtcctacctg ttggagaagg
     4801 agggcttcgc ggtcgcggtc gccgcgaccg gccccgacgc cctcgccgag ttcgacgcca
     4861 acggcgccga cctcgtgctg ctggacctca tgctcccggg cctgtccggc gtggaggtat
     4921 gccgtgcgct ccg
//
LOCUS       DAORPW010000174         4926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4956_length_4926_cov_775.626976, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000174 DAORPW010000000
VERSION     DAORPW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4956_length_4926_cov_775.626976"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /gene="moeB"
                     /locus_tag="PLB86_11095"
     CDS             complement(<1..1163)
                     /gene="moeB"
                     /locus_tag="PLB86_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HRC65728.1"
                     /translation="MDTGSGPPPDVLDDAEARTRYARHLLLPEVGLAGQRRLAAARVL
                     VLGAGGLGSPALLYLAAAGVGTIGIADDDVVELTNLQRQIVHGFPDVGRSKVSSAAAA
                     VARVNPGVRVETFDQRVSAQTADALVAAYDVVLDGTDNFPTRYAVNDACARAGIPLVW
                     ASVLRFDAQVAVWWAGHGPCLRCVFPHQPAEGDVPSCAEAGVLGALCGTIGSLQAAEA
                     LKLVLGIGEPLVGRMLVHDALRQSWDTLALQADPECPACGSPVGGGRGARLEAVALAL
                     PVPAEPVAVVTARELSIEMRRPHPPVLIDVRPEPERAIVSIPGAVAIDLADFRSGEAF
                     SRPELGSLGTPLVLYCKTGVRSTEATRLVAAAGWGYAANLTGGVLAWVREVDPG"
     gene            1288..2013
                     /locus_tag="PLB86_11100"
     CDS             1288..2013
                     /locus_tag="PLB86_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC65729.1"
                     /translation="MVNDLRSGASAAPRATPGAAASPPAPLAAEAPIRGQRLPRSARR
                     AQLLEAAQGAFVESGYYAAAMDDIAERAGVSKPVLYQHFPSKLELYLALLDDRAGELV
                     AAVRSALASSTDNKARVFATVAAYFEFVAKEGSAFRLLFESDLINEDAVSSRIDGALT
                     QCGEAFADAIAADTNMSRADAMVLAMALTGMAQVAARNWATLDTTVGRAEAARLVGQL
                     AWRGLGGFPKAPAEGAGEHPATS"
     gene            2147..2371
                     /locus_tag="PLB86_11105"
     CDS             2147..2371
                     /locus_tag="PLB86_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HRC65730.1"
                     /translation="MEVRIGVRDVAREIAFETNETPAAIGAAVAVALRDGSVLDLADD
                     KGRRYFIPAAVIGFVHVGEAEKGKVGFGAH"
     gene            2551..2805
                     /locus_tag="PLB86_11110"
     CDS             2551..2805
                     /locus_tag="PLB86_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRC65731.1"
                     /translation="MSILGAIVGGLIVGLLARFILPGRQGLSLPITAAIGIVAALIGW
                     WVAGKLGVQTTSGIDWLRWIISTIIAAVGIVIYGKVTSKA"
     gene            complement(2925..3617)
                     /locus_tag="PLB86_11115"
     CDS             complement(2925..3617)
                     /locus_tag="PLB86_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HRC65732.1"
                     /translation="MTDDTGVAGSAAVGEVPHEAVVDLLGALSYAQLSGFEALAADAA
                     MTPHLRAKLAFARMGVEMFHRCEQVGLRLRELGADHESAMAPFVTSVDAFHERTKPSD
                     WLEGIVKAYVGDGIVRDFYSEMATHLDPANEAFVAQMLGQAGGAEAIVHEVREAIAGD
                     ARRSGRLALWARRIMGEAIHATQSVAVERDSLTALLVGGEPGSDLNEVGRMFARISAA
                     HTVRMEALGLSA"
     gene            3873..4313
                     /locus_tag="PLB86_11120"
     CDS             3873..4313
                     /locus_tag="PLB86_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65733.1"
                     /translation="MSTAAPTAPTGTFGDHVAESHLAASASSGTAKKVELFARGASIG
                     WLGENGGQWCVVTGSDAALSLESYLYGGILYFRNAADTSRYLSVSDGYHYVGFYNWIG
                     ATGWVLNGNVLTSLYTNAQLSYWSTDNGYLYANSGYTPLTVVFH"
     gene            4768..>4926
                     /locus_tag="PLB86_11125"
     CDS             4768..>4926
                     /locus_tag="PLB86_11125"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRC65734.1"
                     /translation="MSRYAHRLVPAAAADDVVQEALVRAWSRRATYDPARGSAAPWLL
                     AIVADQARR"
BASE COUNT          796 a         1801 c         1611 g          718 t
ORIGIN      
        1 cccgggtcga cctcgcgcac ccaggccagc accccgccgg tgaggttcgc ggcatacccc
       61 cagccggcgg cggccaccag ccgagtggcc tccgtcgagc gcacccccgt cttgcagtag
      121 aggaccaacg gagtccccag actccccagc tcgggtcgcg aaaaagcttc tcccgagcgg
      181 aaatccgcca gatcgatcgc cacagctccg gggatcgaga cgatcgcacg ctcgggctcc
      241 gggcgcacgt cgatgagcac gggcggatgc gggcgccgca tctcgatcga caactcgcga
      301 gcggtcacca ccgcaacggg ctcagccggg acagggagcg cgagcgcgac ggcctcaagc
      361 cgcgccccac gccctccccc gacgggcgac ccgcaggccg ggcactccgg atctgcctgc
      421 agcgcaagcg tgtcccagct ctgtcgcaac gcgtcgtgca cgagcatccg cccgacgagg
      481 ggttcgccga tgcccagcac gagtttgagc gcctccgcgg cctgcagcga gccgatcgtc
      541 ccgcacaagg cccccagcac ccccgcctcc gcgcaggacg gcacgtcgcc ctcggccggt
      601 tggtgcggga acacgcaccg caggcatggc ccgtgcccgg cccaccacac cgccacctgc
      661 gcgtcgaaac gcagcaccga cgcccacacg agggggatgc ccgcgcgcgc gcaggcgtcg
      721 ttgacggcat accgcgtcgg gaagttgtcg gtcccgtcga gcacgacgtc gtatgccgcg
      781 accagggcgt ccgcggtctg cgcactcacc cgctggtcga aggtctcgac ccgcacgccg
      841 gggttgaccc gcgcgacagc cgccgcagcc gacgacacct tggaccggcc gacatcgggg
      901 aagccgtgga cgatctggcg ctgcaggttg gtcaactcga ccacatcgtc atcggcgatc
      961 ccgatggtcc cgaccccggc ggcagcgagg tagagcagcg ccggcgagcc cagccctccc
     1021 gcgccgagga cgaggacccg ggccgcagcg agccgccgct gtccggcgag gcccacttcc
     1081 ggcagcaaca agtgccgggc ataacgcgtg cgggcctcgg cgtcgtcgag cacgtcgggc
     1141 ggcgggccgg atccggtgtc catcgctagc gaggttacgt catagggctg ggggataccg
     1201 gtgagtacga tggcacctgg cggccggacg gtcgtcggcg gtcgatcgtt cgcacagtcg
     1261 tgcgatacgc acaaggtggt gttgacggtg gtgaacgacc tgcgatcggg agcgtcggct
     1321 gccccgcgtg cgacgccggg agcggcggcc tcgccgcccg ccccgctcgc tgccgaggca
     1381 cccatccgcg gccaacggct gccgcgctcg gcccgccggg cccaactgct ggaggccgcg
     1441 cagggcgcct tcgtcgagtc gggctactac gccgccgcga tggacgacat cgccgaacgc
     1501 gcaggggtgt ccaaacccgt tctctaccag cacttcccga gcaagctgga gctctatctc
     1561 gcactcctgg acgatcgcgc cggcgagttg gtggccgccg ttcgcagcgc gctggccagt
     1621 tccacggaca acaaggcacg ggtcttcgcg acggtcgccg cctacttcga gttcgtcgcc
     1681 aaggaggggt cggccttccg gctcctcttc gagagcgacc tcatcaacga ggacgctgtc
     1741 agcagccgca tcgacggggc gctgacccag tgcggcgagg ccttcgccga cgccatcgcc
     1801 gccgacacca acatgtcccg cgccgatgcg atggtcctgg cgatggccct gacggggatg
     1861 gcacaggtgg ccgcccgcaa ctgggccacc ctcgacacca ctgtgggccg cgccgaagcg
     1921 gcccgcctgg tcggccaact cgcctggcgc gggttggggg gcttccccaa ggcaccggcc
     1981 gagggcgccg gggaacaccc cgccacctcc tagagttgag gggttcgaca ccccaacccg
     2041 ggtatgccgt gcgcccgggt tccccgtgcc accgtcacct ccgcgagggg gcggcgaggc
     2101 gcgaggcgag cacacggcat acgtgaactg acgaagggaa ccccacgtgg aggtcaggat
     2161 cggcgtgcgc gatgtcgccc gcgagattgc gttcgagacc aacgagaccc cggccgccat
     2221 cggcgccgcc gtggccgtcg cgctgcgcga tggcagcgtg ctcgacctcg ccgacgacaa
     2281 gggacggcgc tacttcatcc ctgctgccgt catcggcttc gtccacgtcg gggaggccga
     2341 gaagggcaag gtcggcttcg gcgcgcactg acgtcggggc acccagcgcc ctcgtcccga
     2401 accggccctg ccaccaggtc acgtcaagat cacgagcggc aatcccagga atgcgtctca
     2461 cttcgtgaga gttttgaatc ctggggagcc cgcccccacc cggaccaccg ggcttgacag
     2521 cggccgcggg agcggccaga aggagtggac atgagcatcc tgggggccat cgtcggcggt
     2581 ttgatcgtcg gactgctggc acgtttcatc ctgcctggac ggcaggggct gagcctgccg
     2641 atcacggcag cgatcggcat cgtcgccgct ctcatcggct ggtgggtcgc cggcaagctc
     2701 ggcgttcaga ccacgtcggg gatcgactgg ctgcgctgga tcatcagcac gatcatcgcc
     2761 gccgtcggca tcgtcatcta cggcaaggtc accagcaagg cctgaggcca agcgccgaca
     2821 acagcagtgg ggcccagccg aaacggctgg gccccactgc tgttgcccac tggtgttacc
     2881 cggcgcgttc ttactgcgtg tgctgaccag cgccgtcggg acgatcaggc cgacaggccc
     2941 aacgcctcca tccgcacggt gtgggccgcc gagatccgcg cgaacatccg gcccacctcg
     3001 ttgaggtccg accccggctc gccgccgacc aggagcgcgg tcagcgagtc gcgttcgacg
     3061 gccacgctct gggtcgcatg gatcgcttca cccatgatcc gccgagccca cagcgccagc
     3121 cggccgctgc gccgggcgtc accggcgatg gcttcgcgca cctcgtggac gatcgcttca
     3181 gcgccaccgg cctgtccgag catctgcgcg acgaacgcct cgttcgccgg gtcgaggtgg
     3241 gtggccatct cggagtagaa gtcgcggacg atcccgtcgc cgacgtaggc cttgacgatg
     3301 ccctccagcc agtcgctggg cttggtgcgc tcgtggaagg cgtcgaccga ggtgacgaag
     3361 ggggccatgg cgctctcgtg gtcggccccg agctcgcgca gccgcagccc cacctgctcg
     3421 caccggtgga acatctccac ccccatccgg gcgaaggcca gcttggcgcg caggtgcggg
     3481 gtcatcgcgg cgtcagccgc gagtgcctcg aacccgctga gctgggcata gctcagcgct
     3541 cccagcaggt cgacgacggc ctcgtgcggc acctcgccca cggcagcact ccctgcgacc
     3601 ccggtgtcat cggtcatagg gctgactgta tcggcggtgc ctcaaccgac caacgggccg
     3661 cccgagtcat tgacaggtgc ggtcgccctt cagcggcgca ctcggcgcac ggaaagcaaa
     3721 gaaggaccga attcctttgg gccgtaaggc tcgctggcgg cacagcggtg ctacattcac
     3781 gtcagagacg tcctcactct ccccagcaat tcatgaggac ctccggcaac ccggccgggc
     3841 aactccaact catcattcga atggagaaag ccatgtccac tgcagcaccc accgccccca
     3901 ccggcacgtt cggtgaccat gtcgcagagt cgcacctggc ggcttcggcc tcttccggca
     3961 cagccaagaa ggtcgaactc ttcgcccgcg gtgcgtccat cgggtggctc ggtgagaacg
     4021 gcggccagtg gtgcgtcgtc accggcagcg acgcggccct ctcgctcgag tcgtacctct
     4081 atggcggtat tctctacttc cgcaacgccg cagacacttc tcggtacctg tcggtgagcg
     4141 acggctacca ctacgtcggg ttctacaact ggatcggagc gacaggctgg gtcctgaacg
     4201 gcaacgtgct cacatcgctc tacaccaacg cccaactgtc gtactggtcg accgacaacg
     4261 gctacctcta cgccaacagt ggctacacgc ccttgacggt ggtcttccac tgacagtggc
     4321 cttccactga cgccgacctg atcgtcccct cacgactatc tgagggcgcc tcgatccgaa
     4381 cgtcactaca gtccattcac cgacgcatac tcagccatgc catttcgggg atggcaggtc
     4441 gagaattgca gcgggcattc ctgcagctgc gcctgagtaa tgcccgctgc ccaaatgcgg
     4501 cgaccaacat cgacacacct ccaacaacac caagtggggc ctagccgttt ggctgggccc
     4561 cactgagttg tgcacggctc gtcagccctg caccttccgc cccgcctgca ccagagatga
     4621 cacagtggtg ttgggcgcgg gcactacacg gaccacctcg ctcccggcgt aagaggggtg
     4681 tgacgatgac cggagtgggg cggcataccc ataagatgcc cgacgccgcc gccttcgggg
     4741 cctgggtcgc ccccccacct gccggcgctg tcccgctatg cccaccgcct cgtgcccgcg
     4801 gctgccgctg acgatgtcgt ccaggaggcc ctcgtgcggg cgtggtcgcg gcgggcaacc
     4861 tacgatccgg cccgaggctc ggccgccccc tggttgctcg cgatcgtcgc cgatcaggcc
     4921 cgccgg
//
LOCUS       DAORPW010000175         4903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_4987_length_4903_cov_536.855198, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000175 DAORPW010000000
VERSION     DAORPW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4987_length_4903_cov_536.855198"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..493)
                     /locus_tag="PLB86_11130"
     CDS             complement(206..493)
                     /locus_tag="PLB86_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65735.1"
                     /translation="MDGSVVRHAPGCALDPVNWFRHAIAVAARLALQDVDRAQHDQVS
                     AAALLDTLGLIAELPEWGEVTLREVARQATIMTDDVFVGMCTCGAARDAGL"
     gene            617..>1916
                     /locus_tag="PLB86_11135"
     CDS             617..>1916
                     /locus_tag="PLB86_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRC65736.1"
                     /translation="MSPTPSGLFHDVPIEDLFFSTTDAKGVIDEANEVFARNSRYERS
                     ELLGAPHNIIRHPDMPGAVFKATWDLLGAGHPVCAYVKNLARDGSTYWAFATIVPIAG
                     RFLSVRATPCDRTARDLFESLYATVREAEIAARDAGASAREAADVGSEVLGKALAENG
                     FGSYTELQLDLVPVEVTAREQVCPPLPQLPDGSKTARKILAQVTAARSSLGAFATDIA
                     DSLTLTDTLSRDLRRLGAALAGLDRAVASAAQTLTAATPAMATGSTMVTYASDPVADS
                     ADMTVGAVGAVGTVEADVAALVPIIQSRLGSLRDDVERAGDELASVVRARKHLALGSA
                     IARMQAEAIGRYVVAVEAGAEDPRVSERALSSLSTALLAILDADLLTDREATTAYVDR
                     VARLATEVAGARETTVAWRSLLESTMPRAATNDGSVTDDGS"
     assembly_gap    1917..2016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2017..2304
                     /locus_tag="PLB86_11140"
     CDS             <2017..2304
                     /locus_tag="PLB86_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65737.1"
                     /translation="STMPRAATNDGSVTDDGSEAGNGAVTGDRPEDGSDDHSDAAHES
                     RLAFDVALSAANDQMGRVADAVGAVAATNAALDRDELAAVLGKIVELAAKI"
     gene            complement(2378..3274)
                     /locus_tag="PLB86_11145"
     CDS             complement(2378..3274)
                     /locus_tag="PLB86_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC65738.1"
                     /translation="MSGDRRVHLDRLAVSLVIGCTVVWGLGQVASKVALDEVPPLTQA
                     GLRSIGAALLVLAWAKLRGIPLWERDRTLGPGLVAGALFAAEFAAVYSALQFTTAGRM
                     TVFLYTAPFVVAIGMPFIARAERLTGLALAGLIAAFAGVGFAFADSFTSAGAGSQQWL
                     GDLLAGLAALGWGATTLVIRATALAPIHPAKTLLYQLGVSGVGLTAIGWFVGERAQWP
                     LSMTVAAALVFQVVVVGSTSFVVWFWLIRTYSATKLSAFTLLTPVVALVGGAVLLREP
                     VSPRIVVALVGVCLGLVAVNRR"
     gene            complement(3362..3748)
                     /gene="trxA"
                     /locus_tag="PLB86_11150"
     CDS             complement(3362..3748)
                     /gene="trxA"
                     /locus_tag="PLB86_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRC65739.1"
                     /translation="MSTIDLTAATFEQTVTENDLVLVDFWAAWCGPCRMFAPVFQKAS
                     DENPDVVFAKVDTEAEQALAAMAGITSIPTLMAFREGVLVFAQPGALPAPALAQVIEG
                     VRGLDMAEVHGQVAAQRAQSGAVADA"
     gene            complement(3786..4238)
                     /locus_tag="PLB86_11155"
     CDS             complement(3786..4238)
                     /locus_tag="PLB86_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRC65740.1"
                     /translation="MTFRRLAVIVATVAVAALTVGACSGSPGSGSAPLASAAAATAAP
                     ANGAEVDAAGFAAALKRPGTVVLDVRTPAEFAAGHLAGAVNVDVNAADFRTIVGGLAK
                     DVPYAVYCRSGNRSATALAIMKGLGFTNTFHLGGGIQAWTAAGGAVTQ"
     gene            complement(4415..4660)
                     /locus_tag="PLB86_11160"
     CDS             complement(4415..4660)
                     /locus_tag="PLB86_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65741.1"
                     /translation="MNRTSVGCTERCFTVAPGRSQTVKFTFTRSTAALNTYAGGQLTW
                     SDGTHKVRIWATKYLGSVADAGEASATNPTIVRVDMP"
BASE COUNT          819 a         1676 c         1642 g          666 t
ORIGIN      
        1 gcgtgcgcca cggagtgcgt ctggtggggt gatcaccccc tcgatcgcac tccgtcacac
       61 cctcgggagc ctcagccccc acaaatgacc gtgcgagcgc tatctccggc ccccactgcg
      121 ccccgcgccc ccacaaatga ccgtgcgagc gctatctccg ggtcaggagc gcgatggggg
      181 tgccgcggcg accgacgtac cgaccctaga ggccggcgtc gcgggctgcg ccgcaggtgc
      241 acatcccgac gaacacgtcg tcggtcatga tcgtcgcctg acgagcgacc tctcgcagcg
      301 tcacctcgcc ccactcgggc aattcggcaa tcagcccgag ggtgtccaac agcgccgcgg
      361 ccgacacctg gtcgtgctga gcccggtcga cgtcctgcag ggcgagtcgg gcagcaacgg
      421 cgatggcgtg tcggaaccag ttcaccgggt cgagcgcaca ccctggcgca tgccgcacca
      481 ctgatccgtc catgggccca ttgtgccgat tcacccggtt tctctcgacg tgcgccgacg
      541 cgatggctag gctcgccccg tctcccccgg cgcgcttcgg cccgccatcc gtttcgcacg
      601 ccgaggtctc tcccccatgt caccgacgcc atccggcctg ttccacgatg tcccgattga
      661 ggacctgttc ttctcgacga ctgacgccaa aggcgtcatc gacgaggcca acgaggtctt
      721 cgcccgcaac tcccgctatg agcgaagtga gctcctcgga gcgccccaca acatcatccg
      781 ccaccccgac atgcctggtg ccgtcttcaa ggccacctgg gatctgctcg gcgcgggcca
      841 tccagtgtgc gcgtacgtca agaacctcgc gcgcgacggc agcacctatt gggcgttcgc
      901 gaccatcgtt cccatcgcag ggaggttcct ctcggtgcga gcaacgccgt gcgaccggac
      961 cgcaagggac ctgttcgagt cgctctatgc cacggtgcgc gaagccgaga tcgcagcccg
     1021 ggacgccggt gccagcgccc gcgaagcggc cgacgtgggc agtgaggtgc tcgggaaagc
     1081 gctcgcggag aacggtttcg gctcctacac cgaactccaa ctggatctcg tcccggtcga
     1141 ggtgacggcc cgcgaacagg tctgtccccc gttgccgcag ttgcctgacg ggtccaagac
     1201 ggcacgcaag atcctcgccc aggtcaccgc ggcacgcagt tcactcgggg ccttcgccac
     1261 cgatatcgcc gattcgctca cgctgaccga caccctgtct cgcgacctgc gccgcttggg
     1321 ggccgccctc gccgggctgg atcgcgcggt ggccagtgca gcccagactc tgaccgcagc
     1381 cacgcctgcc atggcgaccg ggtccacgat ggtgacctac gcgtccgacc cggtggccga
     1441 ttcggcagac atgaccgttg gggccgtggg ggcggttggg accgttgagg ccgacgtggc
     1501 cgcactggtg cccatcatcc agagccgact cggctcgttg cgcgacgacg tcgagcgcgc
     1561 aggcgacgaa ctggcttccg tggtccgggc ccgcaagcac cttgcgctcg gcagcgcgat
     1621 cgctcgcatg caggcggagg cgatcgggcg atacgtggtc gctgtcgagg ccggcgccga
     1681 ggacccgcgc gtgtccgagc gcgcgctgtc gagtctgagc acggcactgc tggccatcct
     1741 tgatgccgac ctgctgaccg atcgagaggc gaccactgcc tatgtcgatc gggtggctcg
     1801 gctggcgacc gaggtggccg gggcgcgcga gacgaccgtc gcgtggcgct ccctcctcga
     1861 gagcaccatg ccgagggctg ccaccaacga cgggtcggtt accgacgacg ggtcggnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagca ccatgccgag ggctgccacc
     2041 aacgacgggt cggttaccga cgacgggtcg gaggctggca acggggcggt caccggcgac
     2101 cgccccgaag acggctccga cgaccactcc gacgccgccc acgagtctcg actggccttc
     2161 gacgtcgccc taagtgcagc aaatgatcag atgggccgag tcgccgacgc cgtcggtgcc
     2221 gtggctgcga cgaacgccgc gctggaccgt gacgagttgg ctgccgtgct gggcaagatc
     2281 gtcgagttgg ccgccaagat ctagctcggt cgaggtattc cgccatcagc aggtaatggc
     2341 ggaatacctc gacccggcaa agggcggcga agagggatca gcggcggttg accgcgacga
     2401 gaccgaggca gacgccgacg agggcaacca cgatgcgagg actgaccggc tcgcgcagga
     2461 gcaccgcgcc accgacgagc gcgacgaccg gagtcaacag cgtgaaggcc gagagtttcg
     2521 tcgcggagta ggtccggatg agccaaaacc acacgacgaa gctcgtggag ccgacgacga
     2581 cgacctggaa caccagggcc gcggcgaccg tcatggacag cggccactgc gctcgttccc
     2641 cgacgaacca gccgatggcg gtcaggccga ccccagaaac gccaagctga tagagcaggg
     2701 tcttggccgg gtggatgggc gcgagagccg tcgcacggat caccagcgtc gtcgctcccc
     2761 acccgagtgc ggcgagcccg gctagcaggt caccgagcca ctgctgtgat ccggcgccgg
     2821 ccgaggtgaa gctatcggcg aacgcgaagc ccacaccggc gaaggccgcg atcaggccgg
     2881 ccagggccag cccagtgagc cgctcggcgc gcgcgatgaa gggcatgccg atggcgacga
     2941 cgaacggcgc cgtgtagaga aacaccgtca tccgccccgc ggtcgtgaat tgcagggccg
     3001 aatacacggc ggcgaactcg gccgcgaaca gggctccggc cacgagcccc ggcccgagcg
     3061 tgcggtcccg ttcccacagg ggtatgccgc gcagcttcgc ccaggcgagc accaacaggg
     3121 ccgcgccgat cgagcggagc cccgcttggg tcagcggtgg cacctcgtcc agggccacct
     3181 tggaagccac ctggcccagg ccccagacga ccgtgcaccc gatgacgagc gacaccgcca
     3241 gccgatcgag gtgcaccctg cggtcgccgc tcaccgaggc accctatccg gccgcgacgt
     3301 gggtatgccg ccctgccgtc ccgcggcact cacagcccga gccgcaccag ccaagcgtgg
     3361 gtcaggcgtc ggcgacggcc ccggactgcg cccgctgggc ggccacctgc ccgtgaacct
     3421 cggccatgtc cagacccctg acgccctcaa tgacctgcgc gagggccggc gcgggcagcg
     3481 ccccaggctg agcgaacaca aggacgcctt cgcggaaggc catcaaggtg gggatcgacg
     3541 tgatgccggc catggcggcg agggcctgct cggcctcggt gtcgaccttg gcgaacacga
     3601 cgtcggggtt ctcgtccgag gccttctgga acaccggcgc gaacatccgg cagggcccgc
     3661 accaggcggc ccagaagtcg accaggacga ggtcgttctc ggtcacggtc tgctcaaacg
     3721 tggcagccgt gaggtcgatg gtgctcatga ggctccttgt gcgaatcggt ggaacgtcag
     3781 ccggatcact gggtgacggc ccctccggct gcggtccacg cttgaatgcc gccgccgagg
     3841 tggaaggtgt tggtgaagcc caggcccttc atgatggcca acgcggttgc cgaccgattc
     3901 ccgctgcggc agtacacggc atacgggacg tccttggcca ggccacccac gatggtgcgg
     3961 aagtccgcgg cgttgacatc gacgttgacg gcaccggcga ggtgtccggc agcgaactcg
     4021 gccggggtgc ggacgtccag gacgaccgta ccggggcgct tgagggcggc ggcgaagcca
     4081 gcggcatcca cttcggcccc gttggcggga gccgcagttg ccgccgcggc ggacgccagg
     4141 ggggccgagc ccgagcctgg ggagccggaa cacgcaccga cggtgagcgc cgcgacggcg
     4201 acggtggcga cgatgacggc caacctgcgg aaggtcataa caaggggctc ctgggactcg
     4261 cgcgacacgg ctcgctgggg aagaacaacc aacctgacta gtatagcgca tacccccagg
     4321 ggtatatccc agagggtagg tgacaggccc caaacatcgg ccagggtggc ccggcgagcg
     4381 ccggaaccac cctggcccaa ggagagctct gcggttacgg catgtccacc cgaacgatgg
     4441 tggggttggt tgccgaggcc tcgcccgcgt cagcgaccga accgaggtac ttcgtggccc
     4501 agatgcggac cttgtgagtc ccgtcagacc acgtgagctg cccaccggca tacgtgttga
     4561 gcgctgccgt ggaccgcgtg aaggtgaact tcaccgtctg cgaccggccc ggagcaaccg
     4621 tgaagcagcg ttcggtgcag cccaccgacg tgcgattcat cgatgctcct gacgcgcgta
     4681 gagtgacctg cagggccctg gcagggccga tcctgagaca gggaccttgg tgccgaggct
     4741 aacggccgcc ggcgctcggt gcggggtcaa tcaggtaaag aggctttcag ctcgactgcg
     4801 agcgcctcca gcgtaagagc atcagcgcgg tgcggcagcg tctcggcgag ccctcagagc
     4861 gggtcccccg tcgggcccgc accgctactc gccgggactt ccc
//
LOCUS       DAORPW010000176         4840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5100_length_4840_cov_756.361964, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000176 DAORPW010000000
VERSION     DAORPW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5100_length_4840_cov_756.361964"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="PLB86_11165"
     CDS             <1..1251
                     /locus_tag="PLB86_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC65742.1"
                     /translation="LYAALSTSALGDSILLLVLSMWVKTITGSNAAAGLTFLFMLLPA
                     LVAPALGIWVDRVRAKPLLVWGNVLSALAVLPLVFVRSADQLWIIWTVAFLYGVSFIV
                     LPAGVNGLLKELIADDVLVDANAALQTTKEGFRLFGPLAGAALFAWTGGWLVAVIDAA
                     SFLIAAGVIASVKVQEAAPAPEGGHIWVQLTAGVRHLVGDRALRHTLVGFGIMLLVLG
                     FTESSIYAVLDAFGKPAAFAAVLVTFQGVGAVIGGLTSNLVVRRLGEVGTTALGMVVL
                     AVPLGVIASATNVNVALAALVVVGVSIPWLFVAVTTLVQRRTPQAVIGRASMALEVLM
                     GTPQAISLAVGALLVVFVSFRTIFVIIALATLAGASYIAIMLRREIAADLATSRDRRA
                     EIGGLGPAVDAAPRADLPTPESPA"
     gene            complement(1240..1995)
                     /locus_tag="PLB86_11170"
     CDS             complement(1240..1995)
                     /locus_tag="PLB86_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRC65743.1"
                     /translation="MDPNPAVAHALDVLQLLARHTEPLPAAAIARDLGLPRSSTYRIL
                     MTLADRGFVSHLRESGRFGLGAAAYELGSAYRRQAPLQRIAAPLLARLVDATTHNAHL
                     AVMHGRDVLYVVEERAAGRPRLVTDVGVRLPAELTASGLAMLSRLPAAQVRALYPAAS
                     QLIRREEHGPRTLTELRRALTQTRARGYAVESGTVTEGLASVAVAVVDHTDHPVAAVA
                     VTYPSDVVDTAGRDRLVEQVRRVAAALTARLRG"
     gene            2096..3649
                     /gene="hutH"
                     /locus_tag="PLB86_11175"
     CDS             2096..3649
                     /gene="hutH"
                     /locus_tag="PLB86_11175"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRC65744.1"
                     /translation="MSTPDHPAVTVGDGPLTIDHVVAVARHDARVVISAAAVARMAQT
                     RAVIDGLADDVEPHYGVSTGFGALATRHIPLELRTQLQRSLVRSHAAGSGAEVEREVV
                     RGLMVLRLATLATGRTGIRPVVAQTYAAMLNAGITPVVHEFGSLGCSGDLAPLAHCAL
                     AAMGEGPVRLADGTPSDAASALQDNGIEPVVLREKEGLALINGTDGMLGMLCLAISDL
                     RGLLTVADVTTAMSVEGLLGTDDVFAADLQALRPQPGQALSAANIRRVIADSPIRHSH
                     DTEDCPRVQDAYSLRCAPQVTGAARDTVEHAARIAAYELASAVDNPVVTLDGRVESNG
                     NFHGAPVAYVLDFLAIVAADVASMSERRTDRFLDRSRSNGLPAFLAGDPGVDSGHMIA
                     QYTQAAIVSEMKRLAAPASVDSIPSSAMQEDHVSMGWSAARKLRRSVDGLTRVLAIEL
                     LTAARGLDLRAPLQPSPATGAVVAALREGVAGVGPDRFLAPEIEYAVQLVASGRALAA
                     AESVTGPLA"
     gene            complement(3686..4114)
                     /locus_tag="PLB86_11180"
     CDS             complement(3686..4114)
                     /locus_tag="PLB86_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRC65745.1"
                     /translation="MILDSSAVCAIVLKEAEALAYSNAASEAESVRMAAPTFVEAAMV
                     IDGKGRPELTRSFDEVLDWLHVEIAPFTERQARLAREAFRDFGRGSGHRARLNLGDCF
                     AYALAKDVGEPLLYKGDDFVHTDVRAVFPRGYVEGVSPRP"
     gene            complement(4111..4368)
                     /locus_tag="PLB86_11185"
     CDS             complement(4111..4368)
                     /locus_tag="PLB86_11185"
                     /inference="COORDINATES: protein motif:HMM:NF019324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRC65746.1"
                     /translation="MSLNIKNERVCRLAKEAAALTGQTQVRVLEQALERFIEERSKAA
                     EAQERFRRVQEILASYTVTDEERAATKRFMEEMYDENGLPV"
     gene            4423..>4840
                     /locus_tag="PLB86_11190"
     CDS             4423..>4840
                     /locus_tag="PLB86_11190"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494134.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRC65747.1"
                     /translation="MEGARPVRAPRGTTLTARSWQTEAPLRMLMNNLDPENAERPDDL
                     VVYGGTGRAARDWRSFDAMVRTLTTLAPDETMLVQSGKPVGVFQTHEWAPRVLIANSN
                     LVGDWATWPEFRRLEHLGLTMYGQMTAGSWIYIGTQG"
BASE COUNT          659 a         1752 c         1656 g          773 t
ORIGIN      
        1 ctgtatgccg cgctgagcac ctccgcgctc ggcgactcga tcctgctgct cgtcctgtcg
       61 atgtgggtca agacgatcac cggctcgaac gccgcggctg gcctgacctt cctcttcatg
      121 ctcctcccgg cgctcgtcgc cccggctctg ggcatctggg tcgaccgggt ccgcgccaag
      181 ccgctgctcg tctggggcaa tgtcctctcg gccctcgcag tcctgcccct cgtcttcgtc
      241 cgctcggccg accaactctg gatcatctgg accgtcgcgt tcctctacgg cgtgtccttc
      301 atcgtcctgc ccgccggagt caacggcctc ctcaaggaac tcatcgccga cgacgtcctc
      361 gtcgatgcca acgctgccct gcagacgacc aaggagggct ttcggctctt cggcccgctc
      421 gcgggggcgg ccctgttcgc gtggacgggg ggttggctcg tggcagtgat cgacgccgcg
      481 tcattcctca tcgctgcagg cgtcatcgcc tccgtcaagg tgcaggaggc ggcgcccgca
      541 cccgagggcg ggcacatctg ggtccaactc accgccggag tccgtcacct cgtcggagac
      601 cgagccctgc ggcataccct cgtggggttt gggatcatgc tgctcgtcct ggggttcacc
      661 gagtcgtcca tctatgcggt gctggacgcc ttcgggaagc cggcggcgtt cgccgcggtg
      721 ctggtgacct tccagggggt gggagcggtc atcggtgggc tcacctccaa cctcgtggtc
      781 cgtcggctcg gtgaggtcgg gaccaccgcc ctgggcatgg tggtgcttgc ggtccccctc
      841 ggcgtcatcg cgagcgcgac caacgtgaac gtcgcgctgg ccgcgctcgt cgtcgtcggt
      901 gtctcgatcc cctggctgtt cgtcgccgtg accaccctcg tccagcgccg cacccctcag
      961 gccgtgatcg gccgggcgtc gatggcgctc gaggtgctga tgggcacccc gcaggccatc
     1021 tcgctggccg tcggcgcgct gctggtcgtc ttcgtctcgt tccgcacgat cttcgtcatc
     1081 atcgcgctgg ccacgctggc gggcgccagc tacatcgcga tcatgctgcg ccgcgagatc
     1141 gcggccgacc tcgccacctc gcgtgatcgg cgcgccgaga tcggtgggct cggcccggcc
     1201 gtggatgcgg cgccaagagc tgacctcccg acgcccgagt cacccgcgta gtcgggccgt
     1261 cagcgcagcc gccacccggc gcacctgctc gaccaaccgg tctcggcctg cggtgtcgac
     1321 gacgtcggac gggtaggtca cggcgaccgc ggccaccgga tggtcggtgt ggtcgacgac
     1381 ggcgacggcc accgacgcca agccctcggt cacggtgccg gactccacgg cataccccct
     1441 ggcgcgggtc tgcgtcagcg cgcggcgcag ttcggtgagg gtgcgtgggc cgtgctcctc
     1501 gcgccggatc agttgggacg cagcgggata cagcgcgcgc acctgtgcgg cggggagtct
     1561 cgacagcatg gcgaggccgc tcgcggtgag ctcggcgggc aggcgcaccc cgacgtcggt
     1621 gaccagtcgc ggacgcccgg cggcgcgctc ctcgacgacg tagaggacgt cccggccgtg
     1681 catgacggcg aggtgcgcat tgtgcgtcgt cgcatcgacc agtcgggcga gcagcggcgc
     1741 ggcgatccgc tgcagcggcg cctgccgccg ataggcggag cccaactcgt aggccgcggc
     1801 acccagcccg aaccggcccg actcgcgcag gtggctgaca aagccgcggt ccgcgagggt
     1861 catgaggatg cggtaggtgc tcgaccgggg caggcccagg tcgcgggcga tcgcggcggc
     1921 cggcagcggc tcggtatgcc gtgccagcag ttgcagcacg tcgagggcgt gcgcgacggc
     1981 cgggttcgga tccatgcggt caggctctca cggcttgacc agggtgtctc atatctggga
     2041 cacgatctgg tgtcggcctc tcccagaagg cgacaccgca ccctctaatg ggcacatgag
     2101 cactccagac caccccgcgg tgaccgtcgg cgacggaccg ttgacgatcg accatgtcgt
     2161 cgccgtcgct cggcatgacg cccgcgtcgt catctctgcc gccgccgtgg cccggatggc
     2221 ccagacccgg gcggtcatcg acgggctggc cgacgatgtc gagccgcact acggagtctc
     2281 gaccggcttc ggcgccttgg ccacgcggca catccccctc gagttgcgca cccaactgca
     2341 gcgctcgctc gtccgctcgc acgcggccgg cagcggtgcc gaggtcgaga gggaggtcgt
     2401 ccgcgggctc atggtgctgc gcctggccac cctcgccacc ggccgcacgg ggatccgccc
     2461 cgtcgtcgcg cagacgtatg ccgcgatgct caacgccggg atcacgcccg tcgtgcatga
     2521 attcggctca ctcgggtgct caggcgacct ggctccgctg gcccactgcg cgctggccgc
     2581 catgggcgaa gggccggtcc ggctggccga cggaactccc tcggacgccg cgagcgccct
     2641 gcaggacaac ggcattgagc ctgtggtcct gcgagagaag gaaggcctgg ccctcatcaa
     2701 cggcaccgac ggaatgctcg gcatgctgtg cctggcgatc tcggacctgc gcgggctgct
     2761 caccgtggcc gacgtgacga ccgcgatgag cgtcgagggc ctgctcggca ccgacgacgt
     2821 cttcgccgcc gacctgcagg ccctgcggcc gcagccgggc caggccttgt cggccgcgaa
     2881 cattcgccgg gttatcgccg acagcccgat ccggcactcg cacgataccg aggactgccc
     2941 ccgcgtccag gacgcctact cgctgcggtg cgccccccag gtgacggggg cggcgcggga
     3001 cacggtcgag catgcggcgc ggatcgcggc atacgaactc gcctctgccg tcgacaatcc
     3061 cgtggtgacg ctcgacgggc gcgtggagag caacggcaac ttccacgggg cgccggtcgc
     3121 ctacgtcttg gacttcctcg cgatcgtcgc ggccgacgtc gcgagcatga gcgagcggcg
     3181 caccgaccgc ttcctggacc gctcccgcag caacggactg ccggccttcc tcgcgggcga
     3241 cccgggtgtc gacagcggcc acatgatcgc gcaatacacc caggccgcga tcgtctcgga
     3301 gatgaagcgg ctggccgcgc ccgcgagcgt cgactcgatc ccgtcgtcgg cgatgcagga
     3361 ggaccacgtc tcgatgggct ggtcggctgc ccgcaaactg cggcgctcgg tcgacgggct
     3421 cacccgcgtg ctggccatcg agttgctcac tgcggcacgg gggttggacc tgcgagcgcc
     3481 gctgcagccg agccctgcca ctggcgcggt cgtcgccgcc ctgcgcgaag gcgttgccgg
     3541 ggtgggtcct gaccgcttcc tggcacccga gatcgagtat gccgtgcagc tcgtcgcgtc
     3601 gggccgcgcc ctcgcggcgg ccgagtccgt caccgggccg ttggcctgag gtgtgctgat
     3661 gaggccgagc agcgtccggc gcccatcagg ggcggggcga cacgccttcg acataacccc
     3721 gggggaacac cgcgcggacg tcggtgtgga caaagtcgtc gcccttgtag aggagcggct
     3781 cgccgacgtc cttggccagg gcgtaggcga agcagtcgcc aaggttgagc cgggcccgat
     3841 ggcccgagcc gcggccgaag tcgcggaacg cctcgcgggc gaggcgcgcc tgacgctcgg
     3901 tgaacggcgc gatctcgacg tgcagccagt ccaggacctc atcgaacgac ctcgtcaact
     3961 ccggtcggcc cttgccgtcg atcaccatgg cggcctcaac gaaggtgggt gccgccatgc
     4021 gcaccgactc cgcttcgctc gcggcgttgg agtaggccaa ggcttcggcc tccttcagca
     4081 cgatggcgca caccgccgaa ctgtcgagga tcacaccggg agcccgttct cgtcgtacat
     4141 ctcctccatg aaccgtttcg tggccgctcg ctcctcgtcg gtgaccgtgt atgaggccaa
     4201 gatctcctgg acccgacgga accgctcctg cgcctcggca gccttgctgc gctcctcgat
     4261 gaaccgctcc aaggcttgct cgagcacgcg cacctgggtc tgcccggtga gcgcggccgc
     4321 ctccttggcc aggcggcata cccgctcgtt cttgatgttg agactcaccg gttacacctt
     4381 tctacccata ctgtctacca atctaccctg aaggagtccg ccatggaagg cgcccgcccc
     4441 gtccgtgccc cccgcggcac caccctcact gcccgttcgt ggcagaccga ggctccgttg
     4501 cggatgctca tgaacaacct cgaccccgag aacgccgagc ggcccgacga cctggtcgtc
     4561 tatggcggca ccgggcgggc ggcgcgcgac tggcgcagtt tcgacgcgat ggtgcgcacg
     4621 ctgacgacgc tcgcgcccga cgagacgatg ctcgtccagt cgggcaagcc ggtcggcgtc
     4681 ttccagaccc acgagtgggc gccgcgcgtg ctcatcgcca actccaacct cgtcggcgac
     4741 tgggcgacgt ggccggagtt ccggcgcctg gagcacctcg ggctgacgat gtacggccag
     4801 atgactgccg ggtcgtggat ctacatcggc acccagggca
//
LOCUS       DAORPW010000177         4807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5164_length_4807_cov_974.635943, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000177 DAORPW010000000
VERSION     DAORPW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5164_length_4807_cov_974.635943"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLB86_11195"
     CDS             complement(<1..315)
                     /locus_tag="PLB86_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HRC65748.1"
                     /translation="MTDTIEAAADRKSTKAGLTGLKLAELKDVASSLGITGTAKMRKD
                     DLVAAISASRGGGSSRGSGRASAGSAATSAAAARSAEPAASSDSAASGNSASGGSSAS
                     GGS"
     gene            complement(557..1570)
                     /locus_tag="PLB86_11200"
     CDS             complement(557..1570)
                     /locus_tag="PLB86_11200"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRC65749.1"
                     /translation="MRKVPVGTAIRVRTPASSANLGPGFDSVGLALGVWDQLDVTVVA
                     EPGLRIEVTGSGADGVPRDESHLVYRSMVRGLRELGVEVPSGLLVQTVNAVPHGRGMG
                     SSATAIVSGVAAAQGLSACAAAVAAAAGEGRPGAAGEAVTIDLEAVNSLASELEGHPD
                     NASATVFGGMTLSWSAQDDLPIAVGASEGEAGTRHTVTVRLPLHPDLTAVVFVPETTL
                     ATHTARALLPERVLLRDAARNSARAALLVEAATRSPELLVPATHDWLHQEARRPSYAA
                     SMDLVDRLRADGHAATISGAGPSVLVLTTTDRVDSVRSGADEIWTRLVPGIPAEGVTV
                     RLR"
     gene            complement(1579..2685)
                     /gene="thrC"
                     /locus_tag="PLB86_11205"
     CDS             complement(1579..2685)
                     /gene="thrC"
                     /locus_tag="PLB86_11205"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRC65750.1"
                     /translation="MAHQWRGVIREYADRLPMLAGAPVVTLLEGGTPLIPAAYLSERV
                     GAQVWLKFEGLNPTGSFKDRGMTTAISVAAARGAKVVICASTGNTSASAAAYATKAGM
                     ACAVLVPDGKIAMGKLSQAIAHGATLLQVAGNFDDCLTVARKLAEAYPVELVNSVNPA
                     RIEGQKTAAFEIVDALGDAPDIHCLPVGNAGNITAYWQGYQEYAAGTPGAPGEAGTGG
                     PATHLPRMWGFQAAGAAPIVLGHPVDEPETIATAIRIGNPASWAQAVAARDDSGGRID
                     AVTDEQILDAHRILSGKEGVFVEPGSAASVAGLLARSESGDVPAGARIVCTVTGHGLK
                     DPQWALKRADGTEVVPTQIAAEAYAAAVALGLES"
     gene            complement(2687..4051)
                     /locus_tag="PLB86_11210"
     CDS             complement(2687..4051)
                     /locus_tag="PLB86_11210"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRC65751.1"
                     /translation="MVSEREDVQGAPVRVALIGCGVVGSAVARLLIEHADDLAARVGR
                     PLELVGIGVRRPGRNRGDGLDPALFTTDSADLVTRADVVIEVVGGIEPARTLILRAMA
                     HGASVVTANKALLAEDGPTLYAAAAEHGVDLYFEAAVAGGIPLLRPLRESLAGDSVRK
                     VMGIVNGTTNYVLDKMDTTGQGFADAVSQAQALGYAEADPTADVEGFDAAAKAAILAS
                     LAFHTRVAGKDVHREGITEVTAADIQAAKDMDCVVKLLAICERTDGEDGSPAGVSVRV
                     HPAMIPRRHPLAGVREAFNAVFVEAEAAGQLMFYGRGAGGEPTASAVLGDVVAVARHR
                     VLGGRGPGESAYADLPLLDMGQALTRYHISLDVADRPGVLAQVASAFADHEVSIETVR
                     QQQVVGGAAADAASGSGGSTARASLVVVTHVAPEAALSATVDALAALPVVRGVNSVMR
                     VEGV"
     gene            complement(4091..>4807)
                     /locus_tag="PLB86_11215"
     CDS             complement(4091..>4807)
                     /locus_tag="PLB86_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRC65752.1"
                     /translation="LRLLGLHSHIGSQIFDTSGYEVSARRILRLHAEIAREHGIEMPE
                     LDLGGGFGIAYTSEHDPLPPKSLAAEMADIVDRECRANGVGIPRISIEPGRAIAGPST
                     FTLYQVGTVKQVRLDGGASRSYVSVDGGMSDNVRTALYDADFSCTLAGRASNATPVLT
                     RVVGKHCESGDIVVKDEFLPADIVAGDLLAVPGTGAYCRALSSQYNHTPRPPVVAVRD
                     GKARVIVRRETVEDLLALDV"
BASE COUNT          683 a         1687 c         1766 g          671 t
ORIGIN      
        1 gctgccgccg gacgcgctgc tgccgccgga cgcgctgttg ccactggctg cgctgtcact
       61 ggaggcggcg ggctcggcgc tgcgagccgc tgcggccgac gtggcggccg aacccgcgct
      121 cgcgcgtccc gaaccgcggg aggagccccc gcctcggctc gccgaaatgg cggccacgag
      181 gtcgtccttg cgcatcttcg ccgtgccggt gatgcccagg ctcgatgcca cgtccttgag
      241 ctcggcaagc ttgagccccg tgaggcctgc tttggtcgac ttgcggtcgg ccgcggcctc
      301 aatggtgtct gtcacgaagg atccttcccc tcgttcgtcg gcatcccctg acgggagccg
      361 ggtgctgcgg ccagcatcag ctgaccggtg agttgacccc acggtggacg cgcgcaaggc
      421 ggaccgaggc tgtcaaggcc tgtcgcgtgg gggtggtgcc gccgcgccgt cgcgggccac
      481 tcggccctcc ggtgcggtgc acgctcaatg taacacctcc gccgtcgccc tgcccgcgtc
      541 ccgccgcaga cgcgtgtcaa cgcaaccgaa ccgtcacacc ctcggcgggg atgcccggca
      601 ccaggcgggt ccagatctcg tcggccccgg aacgcacgga atcgacccga tcggtcgtcg
      661 tcaggacgag gacgctcggc ccggcgccgg agatggtcgc cgcgtgtccg tcagctcgca
      721 accggtcgac gaggtccatc gacgcggcat acgaggggcg tcgcgcctcc tggtgcagcc
      781 agtcgtgggt cgcggggacc agcagttcgg ggctgcgggt ggccgcctcg acgagcagcg
      841 ccgcgcgagc cgagttgcgg gccgcgtcgc gcagcagcac gcgctcgggc agcagcgcgc
      901 gggccgtgtg agtggccaag gtcgtttccg gcacgaagac gacagcggtc aggtcggggt
      961 gcaggggcaa ccgcacggtg acggtatgcc gtgtgcccgc ctccccctcc gacgccccga
     1021 ccgcgatggg caggtcgtcc tgggccgacc acgagagggt catccccccg aagacggtcg
     1081 ccgaggcgtt gtccgggtgc ccttcgagct cactggcgag agagttgacc gcctcaaggt
     1141 cgatcgtgac ggcctcccct gccgcgcccg ggcgcccctc gcccgcagcc gccgccacag
     1201 ccgcagcgca cgctgaaagt ccttgcgccg cagcgacccc cgagacgatc gccgttgccg
     1261 acgaacccat cccacgtccg tgcggcaccg cgttgaccgt ctgcacgaga agtcccgacg
     1321 gcacctcgac gccgagctcg cgcaggcctc ggaccatcga gcgatagacc agatgggact
     1381 cgtcgcgggg caccccgtcg gcgcccgacc cggtgacctc gatgcgcaac cccggctcgg
     1441 cgaccacggt gacgtcgagt tggtcccaga ccccgagagc caggccgacg gagtcgaaac
     1501 ccggcccgag gttggcgctg ctggccgggg tccgcacccg gatcgccgtg ccgacgggga
     1561 ccttgcgcat gatgagggtc agctttcgag accgagggcg acggccgcgg catacgcctc
     1621 ggcggcgatc tgggtcggga cgacctcggt gccgtcggcg cgcttgagag cccactgcgg
     1681 gtccttgagg ccgtggccgg tgacggtgca gacgatgcgg gcccccgcgg gcacgtcgcc
     1741 ggactccgag cgggccagca ggcccgcgac cgaggccgcc gagccgggct ccacgaacac
     1801 gccctccttg cccgagagga tccggtgggc gtcgaggatc tgctcgtcgg tgaccgcgtc
     1861 gatccggccg ccggagtcgt cacgggccgc gacggcctgg gcccacgagg ccgggttgcc
     1921 gatccggatg gcggtggcga tggtctcggg ctcgtcgacc gggtggccga ggacgatcgg
     1981 cgccgcgccg gcggcctgga acccccacat tcggggcagg tgggtggccg ggccacctgt
     2041 gcccgcctcg ccgggagcgc cgggggtgcc cgcggcatac tcctggtaac cctgccaata
     2101 ggcggtgatg ttgcccgcgt tgccgaccgg caggcagtgg atgtcggggg cgtcgcccag
     2161 cgcgtcgacg atctcgaagg ccgcggtctt ctgcccctcg atgcgggcgg ggttgaccga
     2221 gttgaccaac tcgaccgggt aggcctcggc gagcttgcgc gccacggtga gacagtcgtc
     2281 gaaattgccc gccacctgca gcaacgtggc cccgtgggcg atcgcctggc tgagcttgcc
     2341 catggcgatc ttgccgtcgg gcaccaggac ggcgcaggcc atcccggcct tggtggcata
     2401 ggcggccgcg ctcgcgctcg tgttgcccgt cgaggcgcag atgacgacct tggcaccgcg
     2461 cgcggcggcc accgagatgg ccgtcgtcat cccgcggtcc ttgaacgagc cggtcgggtt
     2521 gaggccctca aacttcagcc acacctgcgc gccgacgcgc tcggagaggt atgccgcggg
     2581 gatgagtggg gtgccgccct cgaggagggt gacgaccggg gcaccggcga gcatcggcaa
     2641 ccgatcggca tactcgcgga tgaccccgcg ccactggtgg gccatgtcag acaccttcca
     2701 cacgcataac ggaattgacg cctcgaacga cgggcaacgc ggcgagcgcg tcgacggtgg
     2761 ccgagagggc cgcctcgggg gcgacgtggg tgacgacgac gaggcttgcc cgcgcggtcg
     2821 acccgcccga gccgctcgcg gcatcggcgg cagcgccgcc aacgacctgc tgctgacgca
     2881 ccgtctcgat cgacacctcg tggtcggcga aagccgaggc cacctgggcg aggacgccag
     2941 ggcgatcggc gacgtcgagg ctgatgtggt agcgagtgag ggcctggccc atgtcgagca
     3001 ggggcaggtc ggcgtaggcg ctctcccccg gcccgcggcc accgaggacc cggtgccgag
     3061 cgacggcgac gacatcgccg aggacggcgc tggcagtggg ctcgccaccg gcaccgcgcc
     3121 cgtagaacat cagttgaccg gcggcttcgg cctcgacgaa gacggcgttg aaggcctcac
     3181 ggacgccggc gagcgggtgc cgccgcggga tcatcgccgg gtggacgcgc acggaaaccc
     3241 ctgcaggaga gccgtcttcg ccatcggtgc gctcgcagat cgcgagcagc ttgacgacgc
     3301 agtccatgtc cttggcggcc tggatgtcgg ccgcggtgac ctcggtgatg ccctcgcggt
     3361 ggacgtcctt gccggcgacc cgagtgtgga aggcgaggct ggcgaggatc gccgccttgg
     3421 ccgccgcgtc gaagccctcg acgtcggccg tcgggtcggc ctcggcataa ccgagcgcct
     3481 gggcctggct caccgcgtcg gcgaagccct gcccggtcgt gtccatcttg tccaggacgt
     3541 agttggtcgt gccgttgacg atccccatga ccttgcgcac ggagtcgccg gcaagcgatt
     3601 cgcgcagcgg ccgcagcaga ggtatgccgc ccgccaccgc cgcctcgaag tagaggtcga
     3661 cgccatgctc ggcggcggcg gcatacaggg tcgggccgtc ctcggcgagg agagccttgt
     3721 tggccgtgac gaccgaggca ccgtgcgcca tggcgcgcag gatgagggtg cgggcgggct
     3781 cgatgccgcc gacgacctcg atgacgacgt cggcgcgggt gacgaggtcg gcggagtcgg
     3841 tggtgaagag ggccgggtcg agcccgtcac ccctgttgcg ccccggccgg cggacgccga
     3901 tgccgacgag ttccaggggg cgacccaccc gggcggccag atcgtcggcg tgctcgatga
     3961 gcagccgggc caccgccgag ccgaccactc cacagccgat gagggcgacc cgcaccgggg
     4021 cgccctgcac gtcttcgcgt tcactcacca ttgcgtttac tcaccacgag gcaccatgat
     4081 cccgcatccc tcagacgtcc aacgccagga ggtcctcgac ggtctcgcgg cggacgatga
     4141 cccgcgcctt gccgtcccgg acggcgacaa ccggcggcct cggcgtgtgg ttgtactggc
     4201 tggacagggc gcggcaatag gcgccggtgc ccggcactgc gagcaggtcg cccgccacga
     4261 tgtcggcagg gaggaactcg tctttgacga cgatgtcacc actctcacag tgctttccga
     4321 cgacgcgggt gagcaccggc gtcgcgttgg acgcgcgtcc ggccagcgtg caggagaagt
     4381 cggcgtcgta gagcgcggtg cggacgttgt cgctcatgcc cccgtcgacg gagacgtaac
     4441 tgcggctggc acctccgtcg aggcggacct gcttgaccgt gccgacctgg tagagcgtga
     4501 aggtgctcgg cccggcgatc gcccggcccg gctcgatcga gatgcgcggg atgccgaccc
     4561 cgttggcccg acactcccgg tcgacgatgt cggccatctc cgcagccaag gacttgggcg
     4621 gcagcgggtc gtgctcgctg gtgtaggcga tgccgaaccc accaccgagg tcgagctcgg
     4681 gcatctcgat gccgtgctcg cgggcgatct cggcgtgcag gcgcaggatc cggcgggccg
     4741 agacctcata gccggaggtg tcgaagatct gcgagccgat gtggctgtgc aggccgagca
     4801 gccgcag
//
LOCUS       DAORPW010000178         4802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5175_length_4802_cov_774.001475, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000178 DAORPW010000000
VERSION     DAORPW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5175_length_4802_cov_774.001475"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1097)
                     /gene="hisC"
                     /locus_tag="PLB86_11220"
     CDS             complement(3..1097)
                     /gene="hisC"
                     /locus_tag="PLB86_11220"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRC65753.1"
                     /translation="MSTAADGSPAPTLRAALDAVPAYIPGKPASAPEGVTAYKLSSNE
                     NPFPPLDAVRVVLDKTLDVVNRYPDMASVDLVAAIAAFVGCDPANIAVGTGSVGILGQ
                     IVQATCDAGDEIVFAWRSFEAYPIVAALADARAVMVPVDAGQRHQLGLMADAVTERTR
                     VIIVCSPNNPTGTVVHRDELEEFLARVPRSVLVVLDEAYLEFVRDVDAPDAVAVWRAH
                     PNVMVLRTFSKAYGLAGLRVGYAVAHAPVAAALRKCAVPFGINTLAQVAAVASLEARD
                     QLMVRVDSIVAERSRVVAGLAEQGWKLAPSEANFVWFPLGADSGPFAAACEAAGLMVR
                     RYGDDGVRVTIGEPEANTRFLQVCRAFAPR"
     gene            1185..1571
                     /locus_tag="PLB86_11225"
     CDS             1185..1571
                     /locus_tag="PLB86_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRC65754.1"
                     /translation="MKNIAIKTLVNGLALWLAAIVVDGIEFGGGAEWTETLKTVVLVA
                     LVFGVVNAVVKPILKFFATPLIWLTLGLGLLVVNALMLQLTSWLAERLTLAFHVTEFF
                     WDAVIGALIITFVSMVLHAVIPDPKN"
     gene            complement(1607..3316)
                     /gene="paaN"
                     /locus_tag="PLB86_11230"
     CDS             complement(1607..3316)
                     /gene="paaN"
                     /locus_tag="PLB86_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HRC65755.1"
                     /translation="MTTLATTPAHPLVEKYQTVLDEASEALATRSYYSRYPESPSPKV
                     YGENAAAEGLAAYQQHLDAPYAALASQPSDGTEVGSEVSPYGPVLGVRYPRLDLDAAL
                     AAATAAIPAWRDAGARVRAAVCVEMIERINARSFEMANAVMHTSGQPFVMSFQAGGPH
                     AQDRALEAVIAGMVEMERIPASAIWEKPGRDAPLRMRKDYQVVPRGVALVIGCNTFPT
                     WNGYPGLFASLVTGNPVIIKPHPRAALPLAITVEIARDVLRENGFDPALVQLAAENDG
                     DGLAKTLAERPEIAIIDYTGGPFFGAWLEQHGAASGQLVFTEKAGVNTVVIDSTDNLK
                     GMLGNLAFSFTLFSGQMCTTPQNVYVQAEVPTDEGVLTFDEIADRIGAAVARFTADDA
                     KAVEMLGATVNPQVRANAAALEQIASDADGRVVLASREVAHPAYPDAVCRAPGLVALD
                     ASAEATYTKEWFGPVAFVIKTASTEASLERLVSTIREHGAMTAAVYSSDEAVLEQARA
                     AASAAGVALSENLTSSVFVNQTAAFSDYHGTGANPAANASYTDAAFVANRFRVITSRR
                     HIA"
     gene            3416..4597
                     /locus_tag="PLB86_11235"
     CDS             3416..4597
                     /locus_tag="PLB86_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRC65756.1"
                     /translation="MGADVDKLGPVGVIGAGAMGSGIAQVAASAGHPVFLCDAFEGAA
                     ERAQTRIAADLAKRAAQGRITAEDAAAIAARITPVAAATHLPACIVVIEAIAEDLDAK
                     SALFEELGRTQPATTILATNTSSLSIDAIAPAAAHPGRVLGLHFFNPPPAMKLVEVIR
                     GETTTQAVLEAATALVVSWGKVAVRCASTPGFIVNRVARPFYGEAQRMVQEGVADAAT
                     IDACLRSAGFRMGPLELTDLIGQDVNLAVGTSVWNQTSRDPRYAPTPFQQGLVAAGHL
                     GRKSGQGVYTYAADGTSPDNVPDEARVAELLASGVLTNPVARTLAMLVNEAVDLVDRG
                     EAGPADVDLAMTLGTGYPKGPIAWGREIGFLKVRDQLLELAAAFPGGRYRPSPALEKI
                     S"
     gene            4594..>4802
                     /locus_tag="PLB86_11240"
     CDS             4594..>4802
                     /locus_tag="PLB86_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65757.1"
                     /translation="MSTPAAKRTPPPLPGFDLGFLEDQWDDDQASRHIGVEIQLLELD
                     HAVFSLKITDHLVNGHGIVHGGYIFT"
BASE COUNT          733 a         1723 c         1639 g          707 t
ORIGIN      
        1 ggtcagcggg gggcgaaggc gcggcatacc tgcaggaagc gggtgttggc ctccggctcg
       61 ccgatggtga cgcggacccc gtcgtcgccg tagcggcgga ccatcagccc ggccgcctca
      121 cacgccgccg cgaacggccc cgagtcagcc cccaacggga accacacgaa gttcgcctcc
      181 gacggcgcca acttccagcc ctgctcggcc aaccccgcca ccacgcgcga ccgctcggcg
      241 acgatcgagt ccacccgcac catcaactgg tcgcgcgcct ccaacgacgc caccgccgcg
      301 acctgcgcca acgtgttgat cccgaacggc accgcgcact tgcgcaaggc ggccgcgacg
      361 ggagcgtggg cgacggcata cccaacgcgc aagccggcca gcccgtaggc cttggagaag
      421 gtccgcaaca ccatgacgtt cgggtgcgcg cgccacacgg ccaccgcgtc gggggcgtca
      481 acgtcgcgga cgaactccag ataggcctca tcgagcacca cgagcaccga ccgcggcacc
      541 cgcgcgagga actcctccaa ctcgtcgcgg tgcacgaccg tccccgtggg gttgttcggc
      601 gagcacacga tgatgacgcg ggtccgctcg gtcaccgcgt cggccatcag cccgagttgg
      661 tggcgctgcc ccgcgtcgac cggcaccatg accgcgcgcg catcggcgag cgccgcaacg
      721 atcgggtagg cctcgaacga gcgccacgcg aacacgatct cgtcgcccgc atcgcaggtc
      781 gcctggacga tctggccgag gatgccgacc gagccggtgc cgaccgcgat gttcgcgggg
      841 tcgcagccga cgaacgccgc gatcgctgcg acgaggtcga cgctggccat gtccgggtag
      901 cggttgacca cgtcgagcgt cttgtcgagg accacgcgga ccgcgtccag cggcgggaag
      961 gggttctcgt tggacgacag tttgtatgcc gtgaccccct cgggcgcgct cgcgggcttc
     1021 ccggggatgt aggccgggac tgcgtcgagg gcggcgcgca gggtcggggc gggggatccg
     1081 tcggcagcag tgctcatggc gacaaatcta cgacccggcg cgaccggagc agccgcggga
     1141 gccggcccgc cggcgtgacc tggcaccatg acgccatgaa gacgatgaag aacatcgcga
     1201 tcaagaccct cgtcaacggg ctcgcgctgt ggttggcggc gatcgtcgtc gacgggatcg
     1261 agttcggtgg cggggcggag tggacggaga cgctcaagac cgtcgtcctc gtcgcgctgg
     1321 ttttcggcgt ggtcaatgcg gtcgtcaagc cgatcctcaa gttcttcgcg acgcccctca
     1381 tctggctgac cctgggcttg ggcctgctgg tcgtcaacgc gctcatgctg cagttgacct
     1441 cgtggctggc cgagaggttg accctcgcct tccacgtcac cgagttcttc tgggatgccg
     1501 tgatcggcgc gctcatcatc acgttcgtgt cgatggtgct gcacgcagtc atccccgacc
     1561 ccaagaactg atcctcgggg tcgggtgacc gcacgaccga acccaatcag gcgatgtggc
     1621 gccggctcgt gatgacccgg aaccggttcg cgacgaacgc cgcatccgtg taggacgcgt
     1681 tcgccgccgg gttggcgccc gtgccgtggt agtccgagaa cgccgccgtc tggttgacga
     1741 acaccgagct ggtgaggttc tccgacaacg ccacgcccgc cgctgatgcc gccgcgcgcg
     1801 cctgctccaa cacggcctca tcggacgaat agaccgccgc cgtcatcgcg ccgtgctcac
     1861 ggatcgtcga gacgagccgc tccagcgacg cctcggtcga ggccgtcttg atgacgaagg
     1921 ccaccggccc gaaccattcc ttcgtgtagg tcgcctccgc ggaggcgtcc agcgcgacca
     1981 ggccgggcgc gcggcatacg gcatcggggt atgccgggtg cgcaacttcg cgggacgcga
     2041 ggacgacgcg accgtcagcg tcggacgcga tctgctcgag agccgcggcg ttggcccgca
     2101 cctgcgggtt gaccgtcgcg ccgagcatct cgacggcctt ggcgtcgtca gccgtgaatc
     2161 gcgcgactgc cgcgccgatc cggtcggcga tctcgtcgaa ggtcagcacg ccctcgtcgg
     2221 tcgggacctc ggcctggaca tagacgttct gcggggtcgt gcacatctgc ccggagaaga
     2281 gcgtgaacga gaaggcgagg ttgccgagca tccccttgag gttgtcggtc gagtcgatga
     2341 cgaccgtgtt gacgccggcc ttctcggtga agaccaactg gccgctggcc gcgccgtgct
     2401 gctcgagcca cgcgccgaag aacggcccgc ccgtgtagtc gatgatcgcg atctcgggcc
     2461 gctcggcgag ggtcttggcc aggccgtcgc cgtcgttctc agcggcaagc tgcacgagcg
     2521 ccgggtcaaa cccgttctcg cgcaggacat cccgggcgat ctcgacggtg atggccagcg
     2581 gcagcgcagc gcgcgggtgc ggcttgatga tgaccgggtt gcccgtgacg agcgaggcga
     2641 acaggcccgg atagccgttc cacgtcggga aggtgttgca cccaatgacc agcgcgaccc
     2701 cacgcggcac gacctggtag tccttgcgca tccgcagcgg ggcgtcacgc ccgggcttct
     2761 cccagatcgc cgacgcgggg atccgctcca tctcgaccat cccggcgatg accgcctcga
     2821 gcgcgcggtc ctgggcgtgc ggcccaccgg cctggaacga catgacgaac ggctggcccg
     2881 acgtgtgcat gaccgcgttg gccatctcga aggaccgggc gttgatccgc tcgatcatct
     2941 cgacgcacac ggccgcgcgc acccgagcgc cggcgtcgcg ccacgcgggg atggccgcag
     3001 tggccgccgc gagcgccgcg tcgaggtcca gccgcgggta gcgcaccccg agcaccggcc
     3061 cgtaggggga gacctcgctg cccacctcgg tgccgtcgct cggctggctc gccagcgcgg
     3121 catacggggc atcgaggtgc tgctggtatg ccgcgagccc ctcggccgcg gcgttctcgc
     3181 cgtagacctt cggcgacggg gactcggggt agcgggagta gtagctgcgc gtggccaggg
     3241 cctcactggc ctcgtcgagg accgtctggt acttctcgac gagggggtgg gcgggcgtcg
     3301 ttgccaaagt cgtcacaatc ttgatgatat ctgcgagcgc aagagtgcgt cacgtcgaca
     3361 agtggtcggc aacctcacgt ggccagccgc ccgggggtac accaagatgg gccccatggg
     3421 tgctgacgtc gacaagttgg ggccggtcgg agtgatcggc gcgggggcca tgggctccgg
     3481 catcgcacag gtggctgcct cggcgggtca cccggtgttc ctctgcgacg cgttcgaggg
     3541 ggccgccgag cgggcccaga cgcggatcgc cgccgacctg gccaagcggg ccgcccaggg
     3601 gcggatcacc gccgaggacg ccgccgccat cgccgctcgc atcactccgg tggccgccgc
     3661 cacccatctg cccgcctgca tcgtcgtcat cgaggcgatc gccgaggacc tcgacgccaa
     3721 atcggcactc ttcgaggagt tgggccgcac ccagccggcc acgacgatcc tcgccaccaa
     3781 cacctcgagc ctgtcgatcg acgccatcgc cccggctgcg gcgcacccgg gccgggtgct
     3841 cggcctgcac ttcttcaacc cgccgccggc gatgaaactc gtcgaggtca tccgcggcga
     3901 gacgaccacc caggccgtcc tcgaagccgc gaccgcgctg gtggtctcgt ggggcaaggt
     3961 cgcggtccgc tgcgcgtcga cgccggggtt catcgtcaac cgagtggcgc gcccgttcta
     4021 tggcgaggcg cagcggatgg tccaggaggg cgtcgccgac gccgccacca tcgacgcgtg
     4081 cctgcgctcg gcgggcttcc ggatggggcc gctggagctc accgacctca tcggccagga
     4141 cgtcaacctc gccgtcggca cctccgtgtg gaaccagacc tcccgcgacc cccgttatgc
     4201 cccaacgcct ttccaacagg gacttgtcgc cgccggtcac ctcgggcgca agtccggtca
     4261 gggcgtctac acgtatgccg cggacggcac ctcccccgac aacgtccccg acgaggcacg
     4321 cgtcgctgaa ctgctggcca gcggggtgct caccaacccg gtcgcgcgca cgctggcgat
     4381 gctcgtcaac gaggccgtcg acctcgtcga ccggggcgag gccggcccgg ccgacgtcga
     4441 cctcgcgatg acattgggta ccggctaccc caagggtccg attgcctggg gccgcgagat
     4501 cggcttcctt aaggtccgcg accagttgct tgagttggcc gcggccttcc ccggcggccg
     4561 ctaccggccg agccccgcgc tggagaagat ctcgtgagca cccctgccgc caagcgcacg
     4621 ccgcccccgc tgccgggctt tgacctcggc ttccttgagg accagtggga cgacgaccag
     4681 gcctcccggc acatcggcgt cgagatccaa ctcctcgagc tcgaccacgc ggtcttctcg
     4741 ctcaagatca ccgaccacct cgtcaacggg cacggcatcg tccacggcgg ctacatcttc
     4801 ac
//
LOCUS       DAORPW010000179         4770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5245_length_4770_cov_841.334464, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000179 DAORPW010000000
VERSION     DAORPW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5245_length_4770_cov_841.334464"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1146
                     /locus_tag="PLB86_11245"
     CDS             298..1146
                     /locus_tag="PLB86_11245"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRC65758.1"
                     /translation="MRQYLDLLDRVLADGVTKSDRTGTGTMSVFGHQMRFDLAEGFPV
                     LTTKRLHLRSIIGELLWFLRGDTNVRWLQERGITIWDEWADEHGDLGPVYGYQWRSWP
                     TPDGRQVDQIAHVVEAIRRNPDSRRHIVSAWNVADVESMALPPCHALFQFYVRPDVSP
                     AALEAAARGEHTAYPAFLDCQLYQRSADIFLGVPFNIASYALLTAMVAQVTGLRPGHF
                     VHTLGDAHLYLNHLDQARLQLTREPRPLPTLRLTPGLGLDEFDLADVELVGYDPHPGI
                     KAPIAV"
     gene            1171..1635
                     /locus_tag="PLB86_11250"
     CDS             1171..1635
                     /locus_tag="PLB86_11250"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRC65759.1"
                     /translation="MTVGMIAAVSRNGVIGDGVRMPWHLPEDLAHFKHTTMGHTLLMG
                     RRTFDSIGRALPGRRSIVLTRNTRWSHADVEVAHTFAEGLALAGPTELWVVGGGEVYR
                     LAMPYAARLVITEVDQDAEGSVTFPAIDARVWVETSRMPHEGFAFVGYERRA"
     gene            complement(1654..2778)
                     /gene="paaK"
                     /locus_tag="PLB86_11255"
     CDS             complement(1654..2778)
                     /gene="paaK"
                     /locus_tag="PLB86_11255"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HRC65760.1"
                     /translation="MTGLLSPTAGTTTRRATFHPLTVAKVERLTPEATAITFAVPDDL
                     AEAYRFAPGQHLTLRRTVDGAEVRNSYSICQSAHGGRPGTLRVAAAKVPGGRMSPWLV
                     DELAAGDVVDVMTPLGNFTCPVQPDAARHHVAIAAGSGITPILALVSTALEEEPGSRV
                     TLLFGNRRTSTVMFLEELEDLKDRYGARLHLIHVLSREAPDVELFHGRIDKERLTRIL
                     DALVPVEGIDEVYLCGPFGMVQDAQALLSERGFGKEHVHHEIFHVDEDGAPKPAPVIV
                     DTSAPPAAVVTVALDGRTTVIPMPTREESILDATLRARPDAPYSCTGGVCGTCRARVV
                     SGEVRMDRNYALEPDEVAAGIVLACQAHPVSDTVELDYDA"
     gene            complement(2779..3303)
                     /gene="paaJ"
                     /locus_tag="PLB86_11260"
     CDS             complement(2779..3303)
                     /gene="paaJ"
                     /locus_tag="PLB86_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HRC65761.1"
                     /translation="MMATATDVLEAVRAIPDPEIPVITIEGLGILRECRLDEAAGVVH
                     VVITPTYSGCPAMRAIEDQIARVCRAHGLRAVVTSQLSPAWTTDWISPDAREALRRFG
                     IAPPGPADADAGAGERAPRPVSVMIGGGRHTVACPRCGSEATSVISQFGSTACKALRR
                     CDSCGDPFDEFKAL"
     gene            complement(3306..>3478)
                     /locus_tag="PLB86_11265"
     CDS             complement(3306..>3478)
                     /locus_tag="PLB86_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HRC65762.1"
                     /translation="PAQASVEAVVAEATLTMPATPKWRSRGGREGVHSEPLGFLLAEM
                     QYLPRSVPGARW"
     assembly_gap    3479..3578
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3579..3941)
                     /locus_tag="PLB86_11270"
     CDS             complement(<3579..3941)
                     /locus_tag="PLB86_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HRC65763.1"
                     /translation="MSEKETGAAYSAYVLGLADDALIYSHRLGEWLTWAPALEEDMAL
                     ANVSLDLIGQARSLLTHVGSLDGTGRSEDDLAYLRDERDFRNVHLVEQRRGDFGQEMA
                     RMLWFSAYQKALYAALADS"
     gene            complement(3938..4225)
                     /gene="paaB"
                     /locus_tag="PLB86_11275"
     CDS             complement(3938..4225)
                     /gene="paaB"
                     /locus_tag="PLB86_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HRC65764.1"
                     /translation="MSEGTLPLFEVFVRAKRGLSHVHVGSVHASDAAMAVRNARDLYT
                     RRQEGVSIWVVAASDIVASSPDEKDSFFDPAGDKIYRHPTFYEVPEDVEYL"
     gene            complement(4222..>4770)
                     /gene="paaA"
                     /locus_tag="PLB86_11280"
     CDS             complement(4222..>4770)
                     /gene="paaA"
                     /locus_tag="PLB86_11280"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRC65765.1"
                     /translation="PYARAMIRTCKEESFHQRQGFELLWELMRGTPEQQAMAQDATNR
                     WWWPALAMFGPRDTGEHAVTGGHTEQSMAWGIKRFSNDDLRQKFVDMTVPQAARLGVT
                     LPDPDLLWNEERGHYDFGEIDWDEFWRVIKGDGACNAARIERRVSSHEQGAWVREAAT
                     AYAAKQRERADGAVGAGEGSAA"
BASE COUNT          697 a         1645 c         1600 g          728 t
ORIGIN      
        1 cgtcatcggg gtgaccatcg cggtgagcac gcggccgaag atggggctcg tcatgggctc
       61 aggctatcgc gcacgagttc cggggccgga cactgcacac gcgctgcagt cggccgctgc
      121 gggatctggg gcaagaaaca gaaaggtcga ggcgctaccc gctcgggggt ccgggtagcg
      181 cctcgacaac acctacatcg ctggggccgg gtggttcgtt acggcgctac acctcagtta
      241 agtgtgatct aggtcacact gtctgggctg tccccgccgg gtgttacggt gccgaacgtg
      301 cgccagtacc tcgacctcct cgaccgtgtc ctcgccgacg gtgtgaccaa gtccgaccgg
      361 accggcaccg ggacgatgag tgtgttcggc caccagatgc gtttcgacct ggccgagggc
      421 ttcccggtgc tgaccaccaa gcggctgcac ctgcgctcca tcatcggcga gttgctgtgg
      481 ttcctgcgcg gcgacaccaa cgtgcgctgg ctccaagagc gcggcatcac catctgggac
      541 gagtgggccg acgagcacgg cgaccttggg ccggtctatg gctaccagtg gcgctcctgg
      601 cccacccccg acggccgcca ggtcgaccag atcgcccacg tcgtcgaggc aatccggcgc
      661 aaccccgact cccggcgaca catcgtctcg gcgtggaacg tcgccgacgt cgagtcgatg
      721 gcgctgcccc cgtgccacgc cctcttccag ttctacgtcc gccccgacgt ctcccccgcg
      781 gcactcgagg cagccgcgcg aggtgagcac acggcatacc ccgcgttctt ggattgccag
      841 ctctatcagc gctcggccga catcttcctg ggcgtgccgt tcaacatcgc ctcctacgca
      901 ctgctcacgg ccatggtcgc ccaggtgacc gggctgcggc cggggcactt cgtccacacc
      961 ctcggtgacg cccacctcta cctcaaccac ctcgaccagg cccgcctgca gctgacccgc
     1021 gagccgcggc ccctgccgac tctgcggctg accccgggcc tcggcctgga cgagttcgac
     1081 ctcgctgacg tcgagctcgt gggatatgac ccgcacccag gcatcaaggc ccccatcgcg
     1141 gtctgaccgg cgccagagga gactggtcct atgacggttg gcatgatcgc ggcggtgtct
     1201 cgcaacggtg tcatcggcga cggcgtccgg atgccctggc acctgcctga ggacctcgct
     1261 cacttcaagc acacgacgat gggccatacg ttgctcatgg ggcggcggac cttcgactcg
     1321 atcggccgag cgctgccggg gcggcgcagc atcgtcctca cccgcaacac ccgctggtcg
     1381 cacgccgacg tcgaggtcgc ccacaccttc gccgaggggc tcgccctcgc cggccccact
     1441 gagttgtggg tcgtcggcgg cggcgaggtc tatcggctcg cgatgccgta tgccgcccgc
     1501 ctggtcatca ccgaagtcga ccaggacgcc gagggctcgg tgaccttccc cgcgatcgac
     1561 gcgcgggtct gggtcgagac ctcacgtatg ccgcacgagg gcttcgcgtt cgtcggttac
     1621 gagcgccgcg cctgacgcct gacgcctgac tcctcaggcg tcgtagtcga gctcgactgt
     1681 gtcgcttacc gggtgcgcct ggcaggccag gacgatcccc gcggcgacct cgtcgggctc
     1741 aagggcgtag ttgcggtcca tccgcacctc accggacacc acgcgcgcgc gacaggtgcc
     1801 gcagacgccg ccggtgcacg aatagggcgc gtcgggccgg gcccgtagcg tcgcatcgag
     1861 gatgctctcc tcgcgggtcg gcatggggat gaccgtcgtg cgtccgtcga gcgccacggt
     1921 gacgaccgcc gccggcgggg ccgaggtgtc gacgatgacc ggcgccggct tgggcgcccc
     1981 gtcctcgtcg acgtggaaga tctcgtggtg gacgtgctcc ttgccgaaac cacgctcgga
     2041 cagcagcgcc tgcgcgtcct ggaccatccc gaacggcccg cagagataga cctcgtcgat
     2101 gccttcgacc ggcaccagcg cgtcgaggat ccgggtgagt cgctccttgt cgatccgccc
     2161 gtggaacaac tcgacgtcag gagcctcgcg tgaaagcacg tggatgaggt gcagccgggc
     2221 gccgtaacgg tccttgaggt cctcgagctc ctcgaggaac atcaccgtcg aggtccgcct
     2281 gttgccgaac agcagggtga cccgggagcc gggctcctcc tccagcgccg tggagacgag
     2341 cgcgaggatc ggagtgatcc ccgacccagc ggcgatcgcg acgtggtggc gggctgcgtc
     2401 gggttggacc gggcaggtga agttccccag cggggtcatg acatcgacga cgtcgcccgc
     2461 ggcaagctcg tcgaccagcc agggtgacat ccgcccgccg ggcaccttgg ccgccgccac
     2521 ccgcagggtc cccgggcggc caccgtgggc cgactggcag atcgagtagg agttgcggac
     2581 ctcggcgcca tcgaccgtgc ggcgcagcgt gaggtgctga cccggggcga agcgataggc
     2641 ctcggcgagg tcgtcgggga cggcgaaggt gatcgccgtc gcctccggcg tgagccgctc
     2701 gaccttggcc acggtcaagg ggtggaaggt ggctcgccgc gtcgtcgtgc cggcggtggg
     2761 gctgagcagt ccggtcatct agagcgcctt gaactcgtcg aagggatcgc cgcaactgtc
     2821 gcaacggcgc agggctttac aggcggtgga gccgaattgg gagatcaccg aggtcgcctc
     2881 gctgccgcag cgcgggcacg cgacggtatg ccgtccgccc ccgatcatca ccgacaccgg
     2941 gcggggtgcc cgctcaccgg caccggcgtc ggcatcggcc ggcccgggtg gggcgatccc
     3001 gaaccgacgc agggcctcgc gggcatccgg cgagatccag tcggtcgtcc acgccgggga
     3061 cagctgggag gtgacgacgg cccgcaggcc gtgggcacgg catacccggg cgatctggtc
     3121 ctcgatggca cgcatcgcgg gacaacccga ataggtgggc gtgatgacca catggaccac
     3181 cccggccgcc tcgtcgagcc gacactcccg caggatgccc agcccctcga tggtgatgac
     3241 ggggatctcc gggtcgggga tcgcccgcac cgcttccagg acgtcggtgg ccgtggccat
     3301 catcgtcacc agcgggctcc gggcaccgag cgcgggagat attgcatctc ggcgagcagg
     3361 aagcccaacg gctcggagtg cacaccttca cgcccgccgc gcgagcgcca cttgggcgtt
     3421 gccggcatgg tcaacgtggc ttcggcgacg accgcctcga cggacgcctg tgccgggcnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg agtcggcgag cgcggcatac
     3601 agggccttct ggtaggccga gaaccacagc atccgggcca tctcctggcc gaagtcgccg
     3661 cggcgctgct ccacgaggtg gacgttgcgg aagtcgcgtt cgtcgcgcag gtaggccagg
     3721 tcgtcctcgg agcgcccggt gccgtccagg ctcccgacgt gggtcagcag cgagcgcgcc
     3781 tgaccgatga ggtcgaggga gacgttggcc agggccatgt cctcctcgag cgccggcgcc
     3841 caggtgagcc actcccccag ccggtgggag tagatgaggg catcgtcagc gaggcccagg
     3901 acataggcgc tgtatgccgc gccggtttcc ttctcgctca caggtattcg acatcctcgg
     3961 gaacttcgta gaacgtcggg tggcggtaga tcttgtcgcc cgccgggtcg aagaaggagt
     4021 ccttctcgtc ggggctgctg gcgacgatgt cgctggccgc cacgacccag atcgagacac
     4081 cctcttggcg gcgggtgtag aggtcacggg cgttgcgcac ggccatcgcg gcatcggagg
     4141 catgcaccga ccccacgtgg acgtgtgaca ggccccgctt ggcccgcacg aagacctcga
     4201 acagcgggag ggttccctcg ctcatgccgc gctcccttca cccgcgccga cggcgccgtc
     4261 ggcacgctca cgttgtttgg cggcatacgc cgtcgctgcc tcgcgcaccc acgcgccttg
     4321 ctcgtgcgag gagacccggc gctcgatccg ggcggcgttg caggcgccgt cccccttgat
     4381 gacccgccag aactcgtccc agtcgatctc gccgaagtcg tagtgcccgc gctcctcgtt
     4441 ccataacagg tcagggtcgg gcagcgtcac accgagccgg gcggcctgcg gaacggtcat
     4501 gtcgacgaac ttctgacgca ggtcgtcgtt gctgaaccgc ttgatgcccc acgccatcga
     4561 ctgctccgtg tggccgccgg tgacggcgtg ctcacccgtg tcgcgcggcc cgaacatcgc
     4621 cagcgccggc caccaccacc ggttggtcgc gtcctgagcc atcgcctgct gctcgggcgt
     4681 gccccgcatg agttcccaca gcaactcgaa gccctgccgc tggtggaagg actcctcctt
     4741 gcaggtgcgg atcatcgccc gggcgtaggg
//
LOCUS       DAORPW010000180         4698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5379_length_4698_cov_646.804221, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000180 DAORPW010000000
VERSION     DAORPW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5379_length_4698_cov_646.804221"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLB86_11285"
     CDS             <1..285
                     /locus_tag="PLB86_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRC65766.1"
                     /translation="SPYREITRPIIDYVKSLRTDNPRDVVIVYVPEYVVGHWWEQLLH
                     NQSALRLRVRLHFIPGVMVASVPWQLRSSEGQSDDWDDRSVPGRAGNIAP"
     gene            282..1565
                     /locus_tag="PLB86_11290"
     CDS             282..1565
                     /locus_tag="PLB86_11290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRC65767.1"
                     /translation="MSPVSRSSGRQRRYAGPRKPAGPRGESLVGEVFEVEVGPVAHGG
                     FCVARHDGRVVFVRHTLPGERVRARVTEGGTEDRFLRADAVEVLSASADRVDPVCRHA
                     GPGGCGGCDWQHVDLAAQRRLKGAVVAEQLRRLAGIDRSVEVLPVPGDEGGFGWRTRV
                     EFAVDRAGRAGLRPTRSHTVLPIVECPIADPRVLASGVLADDWSGAKAVDVVAPSGGG
                     AVVVVPIPAAAGSVPEIRERVESASWTGEFDVSARGFWQVHPGAAATFVDRVLAELDP
                     RPGERVLDLYAGVGLFAVPLADAVGPGGAVLAVEGDVLACELAVRNAGNRTWIEVVPD
                     RVEEVLASTSPDAWSSADLVVLDPPRTGAGRAVIEAVARLQPRAVAYVACDPAALARD
                     LGYAREAGYVLGGLTAYDAFPMTHHVECIAVLKPT"
     assembly_gap    1720..1819
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1854..2096)
                     /locus_tag="PLB86_11295"
     CDS             complement(1854..2096)
                     /locus_tag="PLB86_11295"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRC65768.1"
                     /translation="MTVLPVNVDDDLIELLDAQAAESGRSRSEVLAAAIRRGLGGGRL
                     AQTLAAYRTGEEISEEDAMALAKDELAASRAERATA"
     gene            2279..>4698
                     /gene="acnA"
                     /locus_tag="PLB86_11300"
     CDS             2279..>4698
                     /gene="acnA"
                     /locus_tag="PLB86_11300"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_170844367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRC65769.1"
                     /translation="MGNVAASVNSFGAKGQLEVGDASYTIFRLAAVDGSATLPYSLKV
                     LLENLLRTEDGANITAEHIRAIGNWDENADPDTEIQFTPARVVMQDFTGVPCVVDLAT
                     MREAVVALGGDPSKINPLAPAELVIDHSVQIDVFGNAGAFEKNVELEYTRNRERYQFL
                     RWGQTAFEEFKVVPPGTGIVHQVNIERLARTVMVRDGLAYPDTCVGTDSHTTMVNGLG
                     VLGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGATTPGVTATDVVLTITDMLRKHGV
                     VGKFVEFYGEGVGTIPLANRATIANMSPEFGSTCAIFPIDDKTIDYLRLTGRADDAVA
                     LVEAYAKDQGLWLDPAAEPRFSEKLELDLSTVVPSIAGPKRPQDRIELSSAKAAFATS
                     LPTYTSDPGKRVPVQMADGRSFELFNGAVSIASITSCTNTSNPSVMIAAGLVARKAAA
                     KGLARKPWVKTSMAPGSKVVTGYYEAAGLWPDLEAIGFYLVGYGCATCIGNSGPLEPE
                     ISEAINANDLAVTAVLSGNRNFEGRISPDVKMNYLASPPLVIAYALAGTMDFDFETDS
                     LGTDQDGNPVYLRDIWPSPEEIQSLMDSSIKKELFTKEYADVFAGDSRWQSLQTPVGK
                     TFEWDGESTYVRKPPYFEGMALEPSPVADIAGARVLAFLGDSVTTDHISPAGSIKVDS
                     PAGKYLAAHGVERKDFNSYGSRRGNHEVMIRGTFANIRLKNLLLDGVEGGFTRDFLDG
                     GAQTTIYDASAAYQAAGVPLVVLAGKEYGSGSSRDWAAKGTRLLGVKAVIAESYERIH
                     RSNLIGMGV"
BASE COUNT          689 a         1570 c         1583 g          756 t
ORIGIN      
        1 tctccctacc gcgagatcac ccggccgatc atcgactacg tcaagtcgct acgcaccgac
       61 aacccccggg acgtcgtcat cgtctacgtg ccggaatacg tcgtcggcca ctggtgggag
      121 caactcctgc acaaccagag tgcgctgcgt ctgcgggtgc gtctgcactt catccctggg
      181 gtcatggtcg ccagcgtgcc ctggcagttg cgctcgtccg aggggcagtc ggacgactgg
      241 gacgaccgct cggtccctgg ccgcgccggc aacatcgccc cgtgagcccc gtctcccgtt
      301 cgtccggccg acagcgccgg tatgccggtc cgcgcaagcc cgcgggtccc cgcggagagt
      361 cgctcgtcgg cgaggtcttc gaggtcgagg tcgggccggt cgcccatggt ggcttctgcg
      421 tcgcccggca cgacggccgc gtcgtctttg tgcggcatac cttgccgggg gagcgagtgc
      481 gcgcgagggt gaccgaagga ggcactgagg accgcttcct gcgggctgac gccgtcgagg
      541 tcctctccgc gtcggcggac cgcgtggatc cggtatgccg ccacgccggc ccgggcgggt
      601 gcggcggatg cgactggcag cacgtcgacc tggccgccca gcgccggctc aaaggtgcag
      661 tcgtggccga acaactgcgg cggctcgccg gcatcgaccg ctcggtcgag gtgctgccgg
      721 tgcctggtga cgagggcggg ttcggctggc gcacgagggt ggagttcgcg gtcgatcggg
      781 ccgggcgggc ggggctgcgt ccgacgcgct cccacacggt cctgccgatc gtggagtgcc
      841 cgatcgccga cccgcgggtg ctggcgagcg gggtgctcgc ggacgactgg tccggtgcga
      901 aggcggtcga cgtcgtcgcg ccctcaggtg gaggagccgt ggtcgtcgtg ccgattccgg
      961 ctgctgccgg cagcgttccc gagatccgcg aacgagtcga atccgcctcg tggacaggtg
     1021 agttcgacgt ctcagcgcgt ggcttctggc aggtgcaccc gggcgcggcg gccaccttcg
     1081 tcgaccgggt cctcgccgag ctcgacccgc gtccggggga gcgggtgctc gacctgtatg
     1141 ccggtgtcgg cctgttcgct gtgccgctgg ccgatgcggt gggcccgggc ggtgcggtgc
     1201 tggccgtcga gggggatgtc ctcgcgtgcg agttggccgt gcgtaatgcc ggcaaccgca
     1261 cctggattga ggtcgtccct gaccgggttg aggaggtgct ggcctcaacg tcgccggacg
     1321 cgtggtccag cgccgatctg gtcgtcctcg acccacctcg gacgggggct gggcgcgcgg
     1381 tcatcgaggc cgtggctcgg ttgcagccgc gggccgtcgc ctatgtcgcc tgtgatccgg
     1441 ccgcactggc tcgagatctg ggctatgcgc gcgaggccgg ctacgtgttg ggtgggctca
     1501 cggcatacga cgccttcccg atgacccacc acgtggagtg catcgccgtg ctgaagccaa
     1561 cctgatccga cctgcggtgc gtccgcgcag cgtgcgaacc agcacccgtc cgtgtcagag
     1621 gccgaaaagg gtcaggagtt cgcgcggtgt gaggacttca atgccgagat tcttggccgc
     1681 atgcaggtcg tcgtcgccgc tggtgagggc gtccgctgcn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnng ggtcgatgac gacccgacgc ggtggagcac cgctcacgcg
     1861 gtggcgcgct cggcccggga ggcggcgagc tcgtctttcg cgagagccat cgcgtcctcc
     1921 tccgagattt cctcgccggt ccggtaggca gccagcgtct gggccaaacg accgccaccg
     1981 agcccgcggc gaatggcagc agccagcacc tcgctgcgtg acctgcccga ctcggccgcc
     2041 tgggcgtcga gcaactcgat caggtcgtcg tcgacgttga ccggaagaac tgtcatcggg
     2101 cacctcctag ggctggtgtc aacaggtggg tgcctagtct acgcgggcgg taccgacagg
     2161 gcggagcctg cgcttggagg gtgttgctgt tctgcaggcg gacaggtagt gcaggatcgt
     2221 aggtggacgt gctaatttat cttgacgtca agatatgtga accaggaagg gagccgacgt
     2281 gggcaacgtc gcagcatcgg tgaacagctt cggggccaaa ggacagctgg aggtcggtga
     2341 tgcgtcctac acgatcttcc gccttgctgc ggtcgacggg agcgcgacgc tgccctacag
     2401 cctgaaggtt ctcttggaga atctcctgcg gaccgaggac ggcgcgaaca tcaccgccga
     2461 gcacatcagg gccatcggca actgggacga gaacgctgac ccggacacag agatccagtt
     2521 cacccccgcc cgcgtcgtca tgcaggactt caccggcgtg ccgtgtgttg tcgacctggc
     2581 gaccatgcgt gaggccgtcg tcgcgctcgg cggcgacccg tccaagatca acccgctcgc
     2641 tccggccgag ttggtcatcg accactcggt gcagattgac gtcttcggca acgcgggcgc
     2701 cttcgagaag aacgtcgagc tggagtacac ccgcaaccgc gagcgctacc agttcctgcg
     2761 ttgggggcag acggcgttcg aggagttcaa ggttgtcccg ccgggcaccg gcatcgtcca
     2821 ccaggtcaac atcgagcgcc tggcccgcac ggtcatggtc cgcgacggct tggcctaccc
     2881 cgacacctgc gttggcactg actcgcacac gacgatggtc aacggcctgg gtgtcctggg
     2941 ctggggcgtc ggcggcattg aggccgaggc cgcgatgctc gggcagccgg tgtcgatgct
     3001 catcccgcgg gtggtgggct tcaagctgac cggcgcgacg acccctggcg tcaccgcgac
     3061 ggatgtcgtc ctcaccatca ccgacatgct gcgcaagcac ggcgtggtcg gcaagttcgt
     3121 cgagttctac ggcgagggtg tcggcacgat cccgctggcc aaccgcgcga cgatcgccaa
     3181 catgagcccc gagttcggct cgacctgtgc gatcttcccc atcgacgaca agacgatcga
     3241 ctacttgcgc ctcaccggcc gggccgatga cgccgtcgcc ctcgtcgagg cctacgccaa
     3301 ggaccagggc ctgtggctcg acccggccgc cgagccccgc ttcagcgaga agctcgagct
     3361 tgacctgtcc acggtcgtcc cgtccatcgc cggcccgaag cgtcctcagg accgcatcga
     3421 gctgtcctcg gccaaggcgg ccttcgccac gtcgctgccg acctacacga gcgaccccgg
     3481 caagcgggtc ccggtgcaga tggccgacgg gcgttccttc gagctgttca acggggcggt
     3541 gtccatcgcg tcgatcacct cgtgcaccaa cacgtccaac ccgtcggtga tgatcgccgc
     3601 gggtctggtc gctcgcaagg cggccgcgaa gggtctggcc cgcaagccct gggtgaagac
     3661 ttcgatggcc cccggctcca aggtcgtcac cggctactac gaggccgccg gcctgtggcc
     3721 ggacctcgag gccatcggct tctacctcgt cggttacggc tgcgcgacgt gcatcggcaa
     3781 ctctggcccc ttggagccgg agatctccga ggcgatcaac gccaacgacc tggccgtgac
     3841 cgcagtcctg tcgggcaacc gcaacttcga gggccggatc agccccgacg tcaagatgaa
     3901 ctacctcgcc tcgccgccgc tggtcatcgc ctacgccttg gcagggacga tggacttcga
     3961 cttcgagacc gactcgctcg gcaccgacca ggacggcaac ccggtctacc tgcgcgacat
     4021 ctggccctcg cccgaggaga tccagtcgct catggactcc tccatcaaga aggagctgtt
     4081 caccaaggag tatgccgacg tcttcgccgg cgactcgcgg tggcagtcgc tgcagacccc
     4141 cgtcggcaag accttcgagt gggacggcga gtcgacctac gtccgcaagc ccccctactt
     4201 cgagggcatg gccctggagc cctcgcccgt cgccgacatc gccggcgctc gcgtcctggc
     4261 gttcctcggt gactcggtga cgaccgacca catcagcccg gccggctcga tcaaggtcga
     4321 cagcccggcc ggtaagtacc tcgcggccca cggcgtcgag cgcaaggact tcaactccta
     4381 cggctcgcgg cgaggcaacc acgaggtgat gatccgcggc accttcgcca acatccggct
     4441 caagaacctc ctgctcgacg gggtcgaggg tggcttcacc cgcgacttcc tcgatggtgg
     4501 ggcgcagacg acgatctacg acgcgtccgc ggcataccag gccgccgggg tgccgctggt
     4561 cgtcctcgcg ggcaaggagt atggctccgg gtccagccgc gactgggccg ccaagggcac
     4621 ccggttgctg ggcgtcaagg ccgtcatcgc cgagagctac gagcggatcc accgctccaa
     4681 cctcatcggc atgggcgt
//
LOCUS       DAORPW010000181         4692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5394_length_4692_cov_710.451369, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000181 DAORPW010000000
VERSION     DAORPW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5394_length_4692_cov_710.451369"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1805)
                     /locus_tag="PLB86_11305"
     CDS             complement(<1..1805)
                     /locus_tag="PLB86_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HRC65770.1"
                     /translation="MTLAIAVVALVATVLTVTELARRLDVSAPLLLTIVGVGASFLPF
                     VPPLVVSPELVLVGLLPPLLYAASVKTSLVDLGANKRNIGMLSVGLVAFSAIGVGLAL
                     WWLFDVPLPLGIAVGGVVAPPDAVAATAVGRRIGLPRRIVTVLEGESLFNDATALVLV
                     RLGILALGSALSVGAVVGQVAWTSIGGVGIGLVVAQVLTWVRRRVTDPISGTAISFLA
                     PWLAYLPAEELHASGVLAVVVAGVLLGHRAPVIQSATSRVADRINWATAQFILENGVF
                     LLIGLQAKGIIERITATPAGVSRALFMSVVVLAAVVVLRPLWVIPLRFLMGDPQDRRD
                     QLRSSVIVSWAGMRGVVTLAAAFTLPVGVPDRDAFVLAALVVVAGTLLVQGSSLPWVA
                     RRLDVHGPDPREDVLQTATVMRSAVTAGLRELDRLAAPTEPPLPDGLVDQLRTMAERR
                     VHAVWEQLGAPSGTERPDGSGGAMVETPSESYRRARTAMLQAERTEVLRLRDLGTIDH
                     EVLSAVLASLDVEESVVTSVSERQGALRERLVLTPESSKGDCLHLREAPVGIEPVTPD
                     GCADCLRDGTTWVHLRLCLTCGNVGCCDSSTGRHAT"
     gene            complement(1856..3568)
                     /locus_tag="PLB86_11310"
     CDS             complement(1856..3568)
                     /locus_tag="PLB86_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRC65771.1"
                     /translation="MTSPSSATTPAPTRTPTAVDAIAENYLARYAALSPIEATYLGLP
                     GHDEDLDDLSPAGYAAMSALRRETLASLADAVAVDDIDLVTIASMRDRLGVAEEKYAA
                     GMDEMSLNVIASPLQNVRDAFDLMPQDTDEHWRTVAIRMAKVPTAMAQYAESLTLARS
                     RGDVAPRRQVDACIVQARDLTADDGYFVTLVAEAKAGDTALDPEVASDLAAAAEAARA
                     AYVQIADFLETELAPVAPAADACGRERYALHSRDFVGAVVDLEETYRWGQEELARITA
                     QMQDVAEQIKPGASIEEAIEVLSADPAYLLEGTDALRTWMQGKADEVITNLADVHFDI
                     PGPVRTIECRIAPTQTGGIYYTGPSDDFTRPGRMWWSVPKGESRFYTWSELTTVYHEG
                     VPGHHLQVAQTVFRRELLNTWRRLASWTSGHGEGWALYAERLMSELGYLDDPGNRMGW
                     LDAQSLRAARVVIDIGVHCGFEAPAEVGGGEWTYDKAWAFLTAHAHESEGKLRFELDR
                     YLGWPGQAPSYKIGERLWLSLREQVRERDGADFDVKAFHRKALDLGGVSLDTLRDAVL
                     GNLG"
     gene            3765..>4692
                     /locus_tag="PLB86_11315"
     CDS             3765..>4692
                     /locus_tag="PLB86_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65772.1"
                     /translation="MFEGLPWEVGQDQLEVLAGRSLRDVSPLLAADALLGVDPLSLLD
                     LDQSVAEEVVAQLQVALNQVAALQAIVIEAVARRVEHDVELAKDSDRLAGHFPAGWGT
                     GDDYTPSVLAPLLHLPPRTMAGRLGEARVLVNELPRTLRAALDGDLEPWRVGAVVAGS
                     FGVASERMPDFEVALFGEGGLGDATAGVLRKRAERVAAKVDAGVLEQRVAEGKRSRCV
                     RVAPGVLPGTTEWTAIAAADRSQLAWSAVCDLADEYLRGKPGLTPGQARADAFLDLLL
                     RNVDVQTVVTIGVTDATIVALLEANEQVQGSGQ"
BASE COUNT          726 a         1562 c         1729 g          675 t
ORIGIN      
        1 gtggcgtgcc gacccgtcga ggagtcgcag cagccgacgt tgccgcaggt caggcacagc
       61 cgcaggtgga cccaggtggt gccgtcgcgc aggcagtcgg cgcagccgtc cggcgtgact
      121 ggctcgatgc cgacgggggc ctcccgcagg tgcaggcagt cgcccttgct cgactcggga
      181 gtgagcacga gccgctcccg cagggcgccc tgtcgctcgg agacggacgt gacgaccgac
      241 tcctcgacgt cgaggctggc cagcaccgct gagagcacct catggtcgat ggtgccgagg
      301 tcgcgcaggc ggaggacctc ggtgcgctca gcctgcaaca tggctgtccg cgcccgccgg
      361 tagctctcgc tgggagtctc gaccatggcg ccacccgagc cgtcgggacg ttctgtcccg
      421 gacggcgcac ccaactgctc ccagacggca tgcacccggc gctcggccat cgtgcgtaac
      481 tggtcgacca acccgtcggg aagcggcggc tcggtcgggg cggccagccg gtccaactcg
      541 cgcagcccag cagtgactgc gctgcgcatc accgtggcgg tctgcaggac gtcctcacgg
      601 gggtccgggc cgtggacgtc gaggcgacga gccacccagg gcaacgacga cccttggacg
      661 aggagggttc ctgcgaccac cacgagggcg gccagcacga aggcgtcccg gtcgggcacc
      721 cccacgggca gggtgaaggc ggccgcgagg gtcacgacgc cgcgcatgcc cgcccacgac
      781 acgatcaccg atgagcggag ctgatcgcgg cggtcctgcg gatcacccat cagaaagcgc
      841 agcgggatca cccagagcgg tcgcagcaca acgaccgcag ccaggacgac aacggacatg
      901 aacagggcac ggctgacccc agcaggtgtc gccgtgatcc gttcgatgat ccccttggcc
      961 tgcagcccga tgagcaggaa gacgccgttc tccaggatga actgggctgt cgcccaattg
     1021 atccggtcgg ccacccgcga ggtggccgac tggatgacgg gggcccggtg gccgaggagc
     1081 acaccggcga cgaccacggc cagcacgccg gacgcgtgca actcctcggc ggggagatag
     1141 gccagccacg gtgcgaggaa ggagatcgcc gttccactga tcgggtcagt gacccggcgg
     1201 cgcacccagg tgagcacctg cgcgacgacg agcccgatcc ccacgccacc gatgctggtc
     1261 caggcgactt gcccgaccac cgcaccgacg ctcaacgcgc tgccgagtgc caggatcccg
     1321 agtcgcacga ggaccagcgc cgtcgcgtcg ttgaagagcg actcgccctc aaggacggtg
     1381 acgatccgcc gcggcagccc gatccggcgg cccacagccg ttgccgccac cgcgtccggg
     1441 ggcgcgacga ccccgccgac ggcaatgccg agcggcagcg gcacatcgaa gagccaccag
     1501 agggccagcc ccacgccgat ggcggagaag gccacgaggc cgaccgagag catcccgatg
     1561 ttgcgcttgt tcgctccgag gtcaaccagt gaggtcttga cggacgcggc atacaacagc
     1621 gggggcagca accccacgag gaccagctcc ggcgacacga cgagcggagg gacaaacgga
     1681 aggaagctcg cgccgacgcc gacaatcgtc agcagcaggg gtgctgacac gtctaggcgc
     1741 cgcgcgagct ccgtcaccgt gagcacggtg gcgaccaacg cgaccacagc gatggcaagg
     1801 gtcacggggc gaatccttca gacactcggg tatgccgccc ggtcggtggc gggggtcagc
     1861 ctaggttgcc caggaccgcg tcacgcaacg tgtcgaggct gacgccgccc aggtcgaggg
     1921 ctttgcggtg gaaggccttg acgtcgaagt ccgcaccgtc gcgctcgcgc acctgctcgc
     1981 gcagcgacag ccacagtcgc tcaccgatct tgtaggacgg cgcctgcccc ggccagccga
     2041 ggtaacggtc caactcgaaa cgcagtttgc cctcggactc gtgggcgtgg gcggtgagga
     2101 aggcccaggc cttgtcatag gtccattcgc cgccgccgac ctccgcgggt gcctcgaagc
     2161 cgcagtgcac gccgatgtcg atgacgaccc gcgccgcacg cagcgactgc gcgtccagcc
     2221 accccatccg gttgccgggg tcgtcgaggt agccgagctc ggacatgagc cgctcggcat
     2281 acagggccca accttcgccg tgccccgagg tccacgacgc gaggcgtcgc caggtgttga
     2341 gcaactcgcg gcggaagacc gtctgggcga cctgcaggtg atgcccggga acgccctcgt
     2401 ggtagacggt cgtcagctcg ctccacgtgt agaacctcga ctcgcccttg ggcaccgacc
     2461 accacatccg cccgggacgg gtgaagtcgt ccgagggccc cgtgtagtag atgccgccgg
     2521 tctgggtcgg cgcgatgcgg cactcgatcg tgcgcaccgg gccggggatg tcgaagtgga
     2581 cgtccgcgag gttggtgatg acctcgtcgg ccttcccctg catccaggtg cgcagcgcgt
     2641 cggtgccttc gaggaggtag gcggggtcgg cgctgaggac ctcgatggcc tcctcgatgg
     2701 aggcaccggg cttgatctgc tcggcgacgt cctgcatctg agccgtgatc cgggccaact
     2761 cctcctggcc ccaccggtag gtctcttcga ggtcgacgac cgccccgaca aagtcgcgcg
     2821 agtgcagcgc atagcgctcg cgcccgcagg cgtcggctgc cggggcgacc ggagccagtt
     2881 ctgtctcgag gaagtcggcg atctgcacgt atgccgcccg cgcggcctcc gccgccgcgg
     2941 ccaggtcgct ggcgacctcc gggtcgaggg ccgtgtctcc cgccttggcc tcggcgacca
     3001 gggtgacgaa gtagccgtcg tccgcggtga ggtcgcgggc ctggacgatg caggcatcga
     3061 cttggcggcg cggcgcgaca tcgccgcggc tgcgagccaa cgtgagcgac tcggcatact
     3121 gcgccatcgc cgtggggacc ttcgccatcc tgatcgcgac cgtgcgccag tgctcgtcgg
     3181 tgtcctgcgg catgaggtcg aaggcgtcgc ggacgttctg cagcggcgag gcgatgacgt
     3241 tgaggctcat ctcgtccatg ccggcggcgt acttctcctc tgccaccccg agccggtcgc
     3301 gcatcgaggc gatggtgacc agatcgatgt cgtcgacggc caccgcatcg gcgaggcttg
     3361 cgagggtctc gcggcgcagc gcggacatcg ccgcgtagcc cgcgggggac aggtcgtcca
     3421 ggtcctcgtc gtggccgggc agcccgaggt aggtggcctc gatggggctc aacgcggcat
     3481 accgggcgag gtagttctcg gcgatggcgt cgacggcggt gggggtgcgg gtgggcgccg
     3541 gggtggtggc gctggaagga gacgtcatgg gctcgaacct acctgctccg gtatgccgcg
     3601 tggcggcaat tgcgcgccgt tgtgtttggg gcgctggggg ccttttgacg agggtggggg
     3661 agcggtgagg aaggtggggc gcgtgtgacg aaagatgttg gcggacaatc taattggcgc
     3721 gaatgatggt tgacatacga acgggtgttc gatagaatgg gggcatgttc gaagggctgc
     3781 cgtgggaggt cggccaggac cagttggagg tcctggccgg ccgatccctg cgcgatgtca
     3841 gcccgttgct ggccgccgat gcgctccttg gcgtcgaccc gctctccctg ctcgacctcg
     3901 accagagcgt cgcggaagag gtcgtcgcgc agttgcaggt tgcgctcaac caggtggccg
     3961 ccttgcaggc gatcgtcatc gaggcggtgg cccgccgggt cgagcatgac gtcgagcttg
     4021 ccaaggacag cgaccggctg gccgggcact tcccggcggg gtggggcacc ggcgatgact
     4081 acacgccatc ggtgctggcg ccgttgctgc atctgccgcc gcggacgatg gccgggcggt
     4141 tgggcgaggc gcgcgtgctg gtcaacgagt tgccgcgcac cctgcgggcg gcgctcgacg
     4201 gcgacttgga gccgtggcgg gtgggggccg tggtcgcggg ctcgttcggg gtggcctcgg
     4261 agcggatgcc cgactttgag gtggcgctgt tcggcgaggg tggcttgggc gacgcgaccg
     4321 ccggcgtcct gcgcaagcgg gccgagcggg ttgccgccaa ggtcgacgcc ggggtgttgg
     4381 agcagcgggt cgccgagggc aagcgctcgc ggtgcgtgcg ggtcgcgcca ggggtcctgc
     4441 cggggaccac ggagtggacc gcgatcgcgg ccgccgaccg gtcccaactg gcgtggagtg
     4501 cggtgtgcga cctggccgat gaatacctgc gtggcaagcc cggtctcacc cccggccagg
     4561 cccgcgccga tgcgttcctc gacctgctct tgcgcaacgt cgacgtccag accgtcgtca
     4621 cgatcggcgt caccgatgcc acgatcgtgg ccttgctgga agcgaatgag caggttcaag
     4681 ggtctgggca gc
//
LOCUS       DAORPW010000182         4674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5423_length_4674_cov_1015.826586, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000182 DAORPW010000000
VERSION     DAORPW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5423_length_4674_cov_1015.826586"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..816)
                     /locus_tag="PLB86_11320"
     CDS             complement(112..816)
                     /locus_tag="PLB86_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65773.1"
                     /translation="MLEVKDLRVSYGKIEAVKGISFSVGQGEVVCLIGTNGAGKTTTL
                     RTISGLIRPTSGEITFEGKRLDGVPAHEIVTLGLAHSPEGRRIFPRLSVEENLLLGAF
                     SRNDGEIKSDLQAAYDLFPILGERSKQPAGTFSGGEQQMLAMGRAMMSRPKLLMLDEP
                     SMGLSPIMMQRIMSTVKTLQSQGTTILLVEQNARAALKLADKGYVLEVGHVVLEGSGH
                     DLLHSDAVRKAYLGED"
     gene            complement(822..1670)
                     /locus_tag="PLB86_11325"
     CDS             complement(822..1670)
                     /locus_tag="PLB86_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC65774.1"
                     /translation="MTTTTQDRPSAPAASGKAVLEATGVIMRFGGLTAVNNVNLRVNE
                     GEIVGLIGPNGAGKTTFFNCLTGMYKPTEGEVKFQGTVLPPKPRAVVQAGMARTFQNI
                     RLFANMTALENVMVGRYCRTSAMALTSIIRGPKYRREEEATRARAQELLNFVGLGKTS
                     EHLARNLPYGDQRRLEIARALATDPKLILLDEPTAGMNPKETEEAKELIFRIRDMGLA
                     VVVIEHDMRFIFTLCDRVLCLVRGETLIEGSPQVVQSDPRVIEAYIGKPDDDELEEEA
                     AKEDQA"
     gene            complement(1667..3376)
                     /locus_tag="PLB86_11330"
     CDS             complement(1667..3376)
                     /locus_tag="PLB86_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC65775.1"
                     /translation="MTTTQSRAAVSNPRPSSQWLLTLVGAVLLVVGCLLPWAFVPAIL
                     GNVTVLLYPGAMQILMIIVGAIAVFFLLLRTGRFPAADRLLDSARGLRALSVTAAAFM
                     VLAVVIIAMQAPGLANADPGVYVSLVGALLMVAGSFRMPSDPKVDPITVHLPSWVEIV
                     VIVVMLAIALFGAAYALGQEDGTIFSLFLLFLAGSVVALARAGLFSWIGVIAQRNRRV
                     ITLGAFAVAFLFPFTQNGSDANMSIATQVLIFATTALGLNIVVGLAGLLDLGYIAFLG
                     AGAYVSAMMSHSAFATIGWKPPFIVVVVIAACVSSILGLIIGSPTLRVSGDYLAIVTL
                     AFGEIFRLTVNNLDGNNGPDLTNGPNGIPGIPELEIGSFNFGDTHTLLGIPLGRFANY
                     YFLMLVVAAIIITIFTRLNNSRIGRGWVAIREDEKAAEAMGVNVFGLKLFAFAGGAFL
                     AGMAGSIKAHQDVSVTPDQYIFLESAFLLAAIVLGGMGTVAGVLVGATVLKLLPEKLR
                     FVADYRLLTFGLLLVLMMRFRPEGLVPSRRRALEFHEDDTKLAERIEEAHLEGMTKTE
                     GRA"
     gene            complement(3373..4296)
                     /locus_tag="PLB86_11335"
     CDS             complement(3373..4296)
                     /locus_tag="PLB86_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC65776.1"
                     /translation="MDQFLQQLLNGVTLGSLYALIAVGYTVVYGIVQLINFAHGEVFM
                     IGAFGSLATWTLFFKGQTGLWVLPFMIIGAVLASVAVALLLERVAYRPLRNAPRLAPL
                     ITAIGISVFLQEAVRLFYPGAKSALPFPQIDLVTGAAFQVGGITVRRSAIFVIGALVV
                     CAVLLWFFINRTKMGRGMLAVSQDPDTARLMGINVDRIIAVAFAIGAALAGVAGVAQG
                     LQNSNIDFFIGFMAGLKAFTAAVLGGIGNIYGAVVGGLVLGVVEAMAVQFIPGTFGGS
                     PWKDVWAFVLLIVVLVFRPQGLMGARVVDRA"
     gene            complement(4453..>4674)
                     /locus_tag="PLB86_11340"
     CDS             complement(4453..>4674)
                     /locus_tag="PLB86_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRC65777.1"
                     /translation="AKAIIEAAKTALKDAKDVKSARTAELTALGKVSFDGVTGKVAFD
                     EFGDTTSKVLTVYKVDGGKWIAAKTDTFK"
BASE COUNT          854 a         1447 c         1631 g          742 t
ORIGIN      
        1 ggcgcggccc gaacagcacg acagtggggc ggggccaact ggccccgccc caccggcata
       61 ccgtgctggt cttgctgcgt tgcgctgtgc tgtcctgacg tgccgtgagc gctagtcctc
      121 gccgaggtac gccttacgga cggcgtcact gtgcagcagg tcgtgtccgg agccttcgag
      181 gacgacgtgg ccgacctcga gcacgtaccc cttgtcagcc aacttgagtg cagcgcgcgc
      241 gttctgctcg acgagcagga tcgtcgtgcc ctggctctgc agggtcttca cggtcgacat
      301 gatgcgctgc atcatgatcg gcgacagacc catggacggc tcgtccagca tgaggagctt
      361 gggacggctc atcatcgccc ggcccatggc gagcatctgc tgctcgccac cggagaacgt
      421 cccggcaggt tgcttggacc gctctccgag gatcgggaac aggtcgtagg ccgcctgcag
      481 gtcgctcttg atctcgccgt cgttgcgcga gaaggcgccc agcaggaggt tctcctcgac
      541 cgagagccgg gggaagatcc gccgaccctc cgggctgtgg gccaggccca gggtgacaat
      601 ctcgtgggcc gggacgccgt ccagacgctt gccctcgaag gtgatctcac ccgatgtggg
      661 cctgatgaga ccggagatcg ttcgcagcgt cgtcgtcttg ccggcaccgt tggtcccgat
      721 gaggcagacc acctcgccct gaccgacgct gaaactgatc cccttgacgg cctcgatctt
      781 gccgtaggag acccgaaggt ccttgacctc gagcatggcg ctcacgcctg gtcctccttt
      841 gcggcctctt cctcgagctc gtcgtcatcg ggtttgccga tgtacgcctc gatgacccgt
      901 ggatcgctct ggacgacctg cgggctgccc tcgatgaggg tctcgccacg gacgaggcag
      961 agcacccggt cgcacagggt gaagatgaac cgcatgtcgt gctcgatgac gacgacggcc
     1021 agccccatgt cgcggatccg gaagatcaac tccttggcct cttcggtctc cttggggttc
     1081 atgccggccg tcggctcatc caggaggatg agcttcgggt cggtggccag cgcccgcgcg
     1141 atctcgaggc gacgctggtc gccgtagggg aggttgcgag cgaggtgttc gctggtcttg
     1201 cccaaaccga cgaagttgag caactcctgg gcgcgagccc gggtggcctc ctcctcgcga
     1261 cggtacttcg ggccacggat gatcgaggtg agggccatgg cgctggttcg gcagtagcga
     1321 ccgaccatga cgttctccaa cgccgtcatg ttggcgaaga ggcggatgtt ctggaaggtg
     1381 cgagccatcc ccgcctggac caccgcccga ggcttgggtg gcaacaccgt gccctggaac
     1441 ttcacctcgc cctcggtggg cttgtacatg ccggtgaggc agttgaagaa cgtcgtcttc
     1501 ccggcaccgt tgggcccgat gaggccgacg atctcgccct cgttgacccg gaggttgacg
     1561 ttgttgacgg ccgtgaggcc gccgaaacgc atgatgacgc cggtcgcctc aaggacggcc
     1621 ttgccggacg ccgcgggggc gctgggacgg tcttgcgtgg tggtggtcat gcccgtccct
     1681 ccgtcttcgt cataccttcc agatgggctt cttctattcg ttcggccaac ttggtgtcgt
     1741 cctcatggaa ctccaaggcg cggcgccgac tcggaaccag gccctccggg cggaaccgca
     1801 tcatgagcac gagcagcaac ccgaaggtga gcaaccggta gtcggccacg aagcgcagtt
     1861 tctcggggag caacttgagc acggtggcac cgacgaggac gccggccacc gtccccatgc
     1921 caccgaggac gatcgcggcg agcaggaagg cggactcgag gaagatgtac tggtcggggg
     1981 tgaccgacac atcctggtgc gccttgatcg acccggccat gccggcgagg aacgcaccgc
     2041 cggcgaaggc gaacagcttg agcccgaaga cgttgacgcc catggcctcg gcggccttct
     2101 cgtcctcacg gatcgcgacc cagccacgcc cgatccggct gttgttgagc cgggtgaaga
     2161 tcgtgatgat gatcgccgcg acgacgagca tgaggaagta gtagttggcg aaccggccga
     2221 gcgggatgcc cagaagggtg tgcgtgtcgc cgaagttgaa cgacccgatc tccaactctg
     2281 ggatgcccgg gatgccgttc gggccgttgg tgaggtccgg gccgttgttg ccgtcgaggt
     2341 tgttgacggt cagccggaag atctcaccga aggcgagggt gacgatggcc aggtagtcac
     2401 cggacacccg cagggttggt gagccgatga tgaggccgag gatcgacgac acgcaggcag
     2461 cgatgacgac gacgacgatg aacggtggtt tccacccgat cgtcgcgaac gcggagtgcg
     2521 acatcatcgc cgacacgtag gccccggcgc cgaggaaggc gatgtagccg aggtcgagca
     2581 ggccggccag gcccacgacg atgttcaggc cgagggcggt cgtcgcgaag atgagcacct
     2641 gggtggcgat ggacatgttc gcgtcggacc cgttctgggt gaacgggaac aggaaggcca
     2701 cggcgaaggc acccagggtg atgacccgac ggttgcgttg cgcgatgaca ccgatccagg
     2761 agaacagccc ggcgcgcgcg agggcgacca cgctgccggc gaggaacagc aggaacagcg
     2821 agaagatcgt gccgtcctcc tgaccgaggg cgtatgccgc gccgaacaac gcgatcgcga
     2881 gcatgacgac gatgacgacg atctccaccc aggacgggag gtgaaccgtg atcgggtcga
     2941 ccttggggtc cgacggcatc cggaaactgc cggcgaccat gagcagtgcg cccacgagcg
     3001 agacgtagac cccagggtcg gcgttggcca gtcccggcgc ttgcatggcg atgatgacga
     3061 ccgcgaggac catgaaggcc gcggccgtca ccgacagcgc acgcaggccg cgggcgctgt
     3121 ccaacaaccg gtcggcggca gggaagcggc ccgtgcgcag gaggaggaag aacacggcga
     3181 tggcgccgac gatgatcatg aggatctgca tcgcgcctgg gtagagcagg acggtgacgt
     3241 tccccaagat ggccgggaca aaggcccagg gcagcaagca gccgacgacg agcagcaccg
     3301 caccgacgag ggtgaggagc cactggctgc tggggcgcgg gttggacacc gcggcacggg
     3361 actgggtggt cgtcatgcgc gatccaccac ccgggcgccc atgaggcctt ggggccggaa
     3421 cacgagcacg acgatgagga gcacgaaggc ccacacgtcc ttccacgggg atccaccgaa
     3481 cgtgccgggg atgaactgga cggccatcgc ctcgacgacg ccgaggacca acccgccgac
     3541 gaccgcacca tagatgttgc cgatgccgcc gaggacggcc gcggtgaagg ccttgagtcc
     3601 ggccatgaag ccgatgaaga agtcgatgtt gctgttctgc agaccctgcg cgacgccggc
     3661 cacgccggca agcgcggcgc cgatggcgaa cgcgaccgcg atgatccggt cgacgttgat
     3721 ccccatgagg cgagcggtgt cggggtcctg ggacacggcg agcatcccgc ggcccatctt
     3781 ggtgcggttg atgaagaacc acagcaggac ggcgcagacg acgagggcac cgatgacgaa
     3841 gatcgccgac cgacggacgg tgatcccgcc gacctggaag gccgcgccgg tgacgaggtc
     3901 gatctgcggg aagggcaggg cgctcttcgc gccgggatag aacagtcgca cggcctcttg
     3961 gaggaacacc gagataccga tggcggtgat gagtggcgcc agccgggggg cgttccgcag
     4021 cggtcggtag gcgacccgct cgaggagcaa cgcgacggcg accgatgcga ggacggcgcc
     4081 gatgatcatg aacggcaaca cccacagccc ggtctggccc ttgaagaaca aggtccatgt
     4141 cgctaaggaa ccgaacgctc cgatcatgaa cacctcgccg tgggcgaagt tgatgagctg
     4201 gacgattccg tagacgacgg tatagccgac agcgatcagt gcatacagcg atccgagggt
     4261 gaccccgttg agtagctgtt gcaagaactg atccatgtca cctcctgagg acgagacggg
     4321 tgttgatgca tggtgattgg ccgaggcccc cctacgtgga acgtaaggcg agagtgtggc
     4381 agcacacagg ggcgagcgct agcgctcgcc cctgtgtgtc tgactattcg tgctatgcgc
     4441 ggttcgggcc gatcacttga aggtgtcggt cttggcggcg atccacttgc caccgtcgac
     4501 cttgtagacc gtgaggacct tcgaggtcgt gtcaccgaac tcgtcgaagg cgaccttgcc
     4561 ggtcacgccg tcgaaggaga ccttgcccag cgcggtcagc tcggcggtgc gagcggactt
     4621 gacatccttg gcgtccttga gggccgtctt ggcggcctcg atgatcgcct tggc
//
LOCUS       DAORPW010000183         4639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5476_length_4639_cov_876.689572, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000183 DAORPW010000000
VERSION     DAORPW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5476_length_4639_cov_876.689572"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLB86_11345"
     CDS             complement(<1..491)
                     /locus_tag="PLB86_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HRC65778.1"
                     /translation="MSTLSPGVAALLAKGVQMPLPETVNVGPEVDPDRVSGRGVTIHP
                     GCRVSGSETVISAGVTLGAEGPVTAIDVRLGPGASIAAGYAAKTVLLEGASLGSGAHA
                     REGTLLEEQASTAHTVGLKQTILLPFVTLGSLINFCDCLMSGGTSRRDHSEVGSSYIH
                     FNFT"
     gene            628..>2567
                     /gene="topA"
                     /locus_tag="PLB86_11350"
     CDS             628..>2567
                     /gene="topA"
                     /locus_tag="PLB86_11350"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRC65779.1"
                     /translation="MAAGKGRKLVIVESPAKAKKIAEYLGGDFEVEASVGHIRDLPNP
                     RDLPPDLKKGPFGKFAVDVDNGFEPYYVVDADKRKKVADLKRLVKESSELYLATDEDR
                     EGEAIAWHLLEALQPRIPVKRMVFHEITKEAIQRAVIDTRELDTRLVDAQETRRILDR
                     LYGYEVSPVLWRKVRQGLSAGRVQSVATRMVVERERERMAFVAASYWDVEAEVTPRAS
                     REPFTARLTGVDGRKVATGRDFGDDGRLKSAAVHLDQAAAHAIAGRLREADVTVTDVA
                     EKPYTRRPAAPFTTSTLQQEASRKLRMSSKHAMRVAQRLYENGYITYMRTDSVTLSGA
                     AVSAARRQARDLYGADYVPGSPRVYTAKVKNAQEAHEAIRPAGDHFRTPAQVAGELRG
                     EDFALYELIWKRTIASQMTDARGSTATVKLGASLGLPAAGVVELSASGTVITFRGFLA
                     AYEEGVDEDRLARAREDEAERRLPQLAVGVPMEVLDAQADGHETTPPPRFTEATLVKA
                     MEEKGIGRPSTYASTVGTIQDRGYVRTRGSALIPTWLAFAVTRLLEEHFTELVDYDFT
                     ASMEEDLDRIANGDEHRAEWLRRFYFGDEASSGQGLRELVEDLGEIDAREISTIHLGD
                     GMVVRVGRYGPYVEELPVEGSTP"
     assembly_gap    2568..2667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2668..3604
                     /locus_tag="PLB86_11355"
     CDS             <2668..3604
                     /locus_tag="PLB86_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HRC65780.1"
                     /translation="DPATPAATPRRATITDDIAPDEMTPAKARELLDAAGDDGRVLGN
                     DPLTGHEIVAKAGRYGPYVTEVIPDPLEAGAGTKHAKKNAKGAKDVKPRTASLFKDME
                     LATIELDVALKLLSLPRVVGLDAEGVEITAQNGRYGPYLKRGTDSRSLVTEAQLFDVT
                     LEEALAIYAQPKTRGGRTAAAPLREFGPDPVSGRPIVLKDGRFGPYVTDGETNATLRK
                     ADDPATMPTERAHELIAEKRARGPVTRKRAAPARKATTKTAAKATTAKTTAAKTTARK
                     TAATKTTAAKTTARKTAATKTTARVAKSTAPRVAD"
     gene            3849..4343
                     /locus_tag="PLB86_11360"
     CDS             3849..4343
                     /locus_tag="PLB86_11360"
                     /inference="COORDINATES: protein motif:HMM:NF039475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6508 domain-containing protein"
                     /protein_id="HRC65781.1"
                     /translation="MARDLRPKDLRSLDPLTTDQVTQAARLHACLQAHHGEWGHWEGG
                     KVGADGAQTPHRVILTPIAQETYDWLLTSGVMPDFNWLAWPAKRELVQGNVSTLEPDV
                     ARALLTALIVQEHSSDGSLLGIFRHGIITTLLGVLGTSPHDPEPEATLDPVDDGQPLV
                     LEPI"
BASE COUNT          776 a         1612 c         1519 g          632 t
ORIGIN      
        1 gtgaagttga agtggatgta gctcgacccc acctcggagt gatcgcgccg agacgtcccg
       61 ccggacatga ggcagtcgca gaagttgatg aggctcccca acgtgacgaa gggcagcagg
      121 atcgtctgct tgagcccgac cgtgtgcgcg gtgctcgcct gctcctccaa gagcgtcccc
      181 tcgcgggcat gcgctcccga cccgagcgag gcgccttcca ggagaacggt tttggcggca
      241 tacccggcgg cgatcgacgc cccggggccg agccgcacgt cgatcgcggt gaccggcccc
      301 tccgccccga gcgtgacgcc tgccgagatg accgtctcgg agcctgacac gcgacaccca
      361 ggatggatgg tcaccccgcg gccactcacg cggtcggggt cgacctccgg cccgacgttg
      421 acggtctccg gcaggggcat ctggacgccc ttggcgagca gggcggcgac ccccggcgac
      481 agcgtgctca tgaccagcca gtatgccgcc cgccgagggg gctgttggaa tccccctggt
      541 cgggtccgct acggtggcgc gggcactgac gtccgagcag cgacgaatcc gctgcacgtg
      601 gtgctcagga aaagcaaggg gtatgccgtg gcagcaggga agggccgcaa gctcgtcatc
      661 gtcgagtcgc cggccaaggc caagaagatc gccgagtacc tcggcggtga cttcgaggtc
      721 gaagcatccg tcggtcacat ccgtgatctg cccaacccga gggaccttcc gccggacctc
      781 aagaaggggc cgttcggcaa gttcgccgtc gacgtggaca acgggttcga gccgtactac
      841 gtggtggacg cggacaagcg caagaaggtc gccgacctca aacgcctggt caaggagtcc
      901 tccgagctct acctcgccac cgatgaggac cgcgaaggcg aggcgatcgc ctggcacctg
      961 ctggaggcgc tgcagccgcg gatccccgtc aagcgcatgg tgttccacga gatcaccaag
     1021 gaagccatcc agcgcgccgt catcgacacc cgagagctgg acacccggct ggtcgatgcg
     1081 caggagaccc gccgcatcct cgaccggctc tacggctacg aggtctcgcc ggtgttgtgg
     1141 cgcaaggtcc gccagggcct gtcggccggg cgtgtgcagt cggtcgcgac gcggatggtc
     1201 gtcgagcgcg aacgcgagcg gatggcgttc gtcgccgcgt cctactggga tgtcgaggcc
     1261 gaggtcaccc cgagggccag ccgcgagccg ttcactgccc gcctcaccgg cgtcgacggc
     1321 cgcaaggtgg ccaccggccg tgacttcggc gacgacggcc ggctcaagag tgccgccgtc
     1381 cacctcgacc aggccgccgc ccacgcgatc gccggccggt tgcgcgaagc cgacgtcacc
     1441 gtcaccgacg tcgccgagaa gccctacacc cgtcgcccgg ccgcgccgtt caccacctcg
     1501 acgctgcagc aggaggcctc ccgcaagctg cggatgtcgt ccaagcacgc gatgcgcgtc
     1561 gcccaacggc tctatgagaa cggctacatc acctatatgc ggaccgactc ggtcaccctg
     1621 tcgggtgccg ccgtctcggc cgcgcgacgc caggcccgcg acctctacgg cgccgactat
     1681 gtgccgggtt cgccgcgcgt ctacacggcg aaggtgaaga acgcccagga ggcccacgag
     1741 gccatccgcc ccgccggtga ccacttccgc accccggcgc aggtcgccgg agagttgcgc
     1801 ggcgaggact tcgcgctcta cgagttgatc tggaagcgca cgatcgcctc ccagatgacc
     1861 gacgcgcgcg gatccaccgc gaccgtcaaa ctcggcgcct cgttgggcct gcctgccgcc
     1921 ggtgtcgtcg aactgtcggc gagcgggacg gtcatcacct tccgtggctt cctcgcggca
     1981 tacgaggaag gcgtcgacga ggaccgcctg gcccgcgccc gcgaggacga ggccgagcgt
     2041 cgccttccgc agttggccgt gggcgtgccg atggaggtgc tcgacgcgca ggcggacggt
     2101 cacgagacca cgccgccgcc gcgtttcacc gaggccaccc tcgtcaaagc catggaggag
     2161 aaggggattg gtcgaccgag cacctacgcc tccacggtcg gcaccatcca ggaccgcggc
     2221 tatgtgcgca cccgcggctc ggccctcatc cccacgtggt tggcgtttgc ggtgacccgc
     2281 ctgctcgaag agcacttcac cgagttggtg gactacgact tcaccgcctc gatggaggag
     2341 gacctcgacc gcatcgccaa cggtgacgag caccgggcgg aatggctgcg gcggttctac
     2401 ttcggtgacg aagcctcgtc gggccagggc ctgcgcgagc tcgtcgaaga cctcggcgag
     2461 atcgatgccc gggagatctc gacgatccac ctcggcgacg ggatggtcgt gcgggtcggg
     2521 cgctacggcc cgtatgtcga ggagcttccc gtcgagggct cgacgccnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnaga tccggcgacc cctgcggcca cgcctcgacg
     2701 agcgacgatc accgacgaca tcgcccccga cgagatgacg ccggccaagg cccgcgaact
     2761 gctcgacgcc gccggcgacg acggccgggt gctcgggaac gacccgctga ccggccacga
     2821 gatcgtggcc aaggcgggcc gatacggccc ctacgtcacc gaggtcatcc ccgacccgct
     2881 cgaggccggc gccgggacga agcacgccaa gaagaacgcc aagggcgcca aggacgtcaa
     2941 gccgcggacg gcgtcgctgt tcaaggacat ggagctcgcc acgatcgagc tggacgtcgc
     3001 cctcaagctg ctctccttgc ctcgcgtcgt cggtctcgac gccgaagggg tcgagatcac
     3061 cgcgcagaac ggccgctatg gcccctatct caagcgcggc accgactctc ggtccttggt
     3121 gacggaggcg caactcttcg acgtcaccct cgaggaggcg ctggcgatct acgcccagcc
     3181 caagacccgc ggaggccgca cggccgccgc gccgttgcgc gagttcggtc ctgaccccgt
     3241 ctccggccgt ccgatcgtcc tcaaagatgg tcgcttcggg ccctacgtca ccgatggcga
     3301 gaccaacgcc acgctgcgca aggccgacga tccggcgacg atgcccaccg agcgcgcgca
     3361 cgagctcatc gccgagaagc gggcccgtgg tccggtcacc cgtaagcggg ccgctccggc
     3421 gcgcaaggcg acgaccaaga ccgccgccaa ggccacgact gctaagacga cggcagccaa
     3481 gacgaccgcg cgcaagaccg cggcgaccaa gacgaccgcg gccaagacga cagcgcgcaa
     3541 gaccgcggcc acgaagacga ccgctcgggt cgccaagtcc acggcacctc gcgtcgcgga
     3601 ctgaggtcgt ccgggagtat gccgtccggc cacctcccgc ccgaatctcg gcttgcagca
     3661 acgggactca tcgtcccgcg gggtgatttg ccgaacgtta atccacggtt caacgaccag
     3721 ggctacccgc gtgtgggttc gcgagtaacc tggggaggtt ggccagagtc gcccgatcgt
     3781 tgaccggacg gcatacgagc gttcgcacct ccgtgaggcg gtggaccgag cgcgagtgag
     3841 gaggaaccat ggcgcgtgat ctgcgaccga aggacctgcg atctctggac cccctgacca
     3901 ctgatcaggt cacccaggcc gctcggttgc acgcctgcct gcaggcccac cacggtgagt
     3961 gggggcactg ggagggcggc aaggtcggag ccgacggggc gcagacccct catcgcgtca
     4021 tcctcacgcc gattgcgcag gagacctacg actggctgct gacctcaggg gtcatgccgg
     4081 acttcaactg gctggcgtgg ccggccaagc gtgagttggt ccaaggcaac gtgtcgacgt
     4141 tggagcccga cgtcgcccgg gcgttgctga cggcgctcat cgtgcaggag cactcgtccg
     4201 acgggtcgct gctggggatc ttccggcacg ggatcatcac caccctgctc ggggtcctcg
     4261 gcactagtcc gcacgaccct gagcccgagg cgaccctaga cccggttgac gacgggcaac
     4321 ccctggtcct ggaacccatc tgacccagca gacggctggg tggcggcata ccctgcgtgg
     4381 gtactacgac caaagcccga tggcggctgc ccgcgctcct ggcgcacctt taagtcgtgg
     4441 ccggaccacc ccggcccacc acctgaaggg aacccaccat gaagcgcatc gttatggcga
     4501 tcgcagcgac ggcggcagtc gtgcccgcag ccctggggtt gctcggcaac gaatcgttcg
     4561 cccagtcgtt gcccaccgca gtcccggcct cagtgcaact cgtcagcagc tcgtcgagca
     4621 ctccagcggg gaccgctga
//
LOCUS       DAORPW010000184         4631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5495_length_4631_cov_608.613199, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000184 DAORPW010000000
VERSION     DAORPW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5495_length_4631_cov_608.613199"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..682)
                     /locus_tag="PLB86_11365"
     CDS             complement(74..682)
                     /locus_tag="PLB86_11365"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC65782.1"
                     /translation="MTEDSRRRVGRPADEPDARDRIMAAAIAEFSRNGFNGTSLERIA
                     RGAGLSKAGVLHHFGSKAAIPFAFLGDRDDVGLRLTEHEADFPEVLTSLVTVARRDVA
                     ASTETRVYAVLSGEAVGIDSPLRPWFQQRYAALQRQVAGGLRRAQDRGRLREGVDIDA
                     AAAEALAVMDGLQLQYLLDDDADTYLARFAAYIDRLVVSLSA"
     gene            762..2129
                     /locus_tag="PLB86_11370"
     CDS             762..2129
                     /locus_tag="PLB86_11370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC65783.1"
                     /translation="MSQRNSAAAPLATEQREPTKAFLWIYVLAYFGGWLGILGPAILA
                     LYTRLADLFPPAGQGTTAVSALAVVSGVGAVAATLANPFIGRLSDRTTSRFGMRTPWL
                     IISSVIFVLGSLVVAFGPANIAVVTIGWSLSQVGFNGMLAIFTAVLPDQIPERMMGRV
                     SSVLGIAMNVASLAAVWLVGLFAAGTITFDDAGVITGGDPSSPLRFIVPVAVAVATSM
                     ILVVALRPIDRHIQRSQVPPYSVGEFFGSFVFNPRKNPDFTWAWVSRFMFMLGIAYLL
                     VYQAPYSSAHLKFEGADLDRVVLWGTVVTVVGTVLTGYVAGQLSDRFKRRKPFVIGSA
                     MGYAVSLVFLSLAAPDNSGLPMALVGLFLGGLAQGVYFGIDLALVTDVLPDREADAAK
                     DLGVFNIASAGPQFIAPFIAPVFLGLNLLGSGANQDNFAALFVFAAIFSAIGALLVLP
                     IKAVR"
     assembly_gap    2161..2260
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2261..>4631
                     /locus_tag="PLB86_11375"
     CDS             <2261..>4631
                     /locus_tag="PLB86_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRC65784.1"
                     /translation="IDELLGRLTLEQKVELITGASFWTTAACPEIGLRSLTLSDGPAG
                     VRGRFWSEQHPSAALPNAVCLAATFDPELVERAGRLIGSEARRNGVDVLLGPTVNLHR
                     SPHGGRNFEAFSEDAVLTAQTGGALVDGIQSQGVAATAKHFIGNDSETERMTLDAVID
                     EATLRSTYALPFEELVVDHDVAAVMSSYNSINGTSGTESPLLESLLRAEWGFASLVIS
                     DWGAVRSVEAAAVARTDLAMPGPESPWSEGLVAAVRAGRVDEADVDVKVASLLGLAAR
                     LGALSGIPPRQDPTALDDPHDPSSPAVRDLLATIAARGAVLLRNEGATLPLEPSPASI
                     AVIGPAATRLRVGGGGSATVVPPHVVSVVTGIEAAFPDALVHAVEGVTQHRLLRPIGD
                     RSTNDLGAAGYDVELLDLEGTVLAAQARTGGELIYGMGFPDGVDGDLVTRIRVTMTLH
                     ETGPRRIGVAGIGDAEIWIEGQRVAAQTLRNEHDDEIAGLSRPPQLVLDVSLTGGERV
                     VIEFAPAPDTIAALRIGLEGPARSTADLLEEAVAAARAADVAVVVVGTTEAEESEGFD
                     RESLSLGADIDALVEAVAAVNPRTVVVLNVGAPVLLPWRERVAAILLAHFPGQEGGTA
                     IGRILAGAEEPGGRLPSSWPRTADSRVPSVTPVAGTLAYQEGEAFGYRDPEGDFAFVF
                     GDGLGFTTWSRSPSSAALLGDGSLRVSASVENTGSRRGRQPVLVFVAPAGSPEALRFA
                     GSTMVDLAPGTSTVAEVVVPSRRLQRLLPSPEAGLIVSLGFSVRDREPAR"
BASE COUNT          594 a         1627 c         1552 g          758 t
ORIGIN      
        1 acccggcaac cgggtgtgat tccgcttcac caccagtctg gcatggcccg ctcgctccgg
       61 ctggtcgacg tgttcaggcg ctcaggctca cgaccaggcg gtcgatgtat gccgcgaacc
      121 tggccaggta ggtgtcggca tcgtcgtcga gcagatactg caactgcaga ccgtccatga
      181 cggccaatgc ctcggcggcg gcggcgtcga tgtcgacccc ctctcggagg cggcccctgt
      241 cttgcgcccg tcgcaggcca cctgcgactt gccgctgcag cgcggcatac ctctgctgga
      301 accagggacg cagggggctg tcgatgccca cggcctcgcc ggagagcacg gcgtagaccc
      361 gggtctcggt ggaggccgcg acgtcccgtc gagcgacggt gaccaggctg gtcaacacct
      421 cggggaagtc ggcttcgtgc tcggtcagcc gcaatcccac gtcgtcgcgg tctccgagga
      481 aggcgaacgg gatcgcggcc ttgctgccga agtgatgcag cacacccgcc ttggagagac
      541 ccgcgccgcg cgcgatccgc tccagcgagg tgccgttgaa accgttgcgg gagaactccg
      601 cgatcgccgc tgccatgatc cggtctcggg cgtcgggctc gtcggccgga cgcccgaccc
      661 ggcggcggga gtcctcggtc atcacccgag cataaagggt cttgcgctta ccaaccggtt
      721 ggtgggtagt atgcgccatc acccctctcg aggagacaac gatgtcccaa cggaactcag
      781 cggccgcgcc cctcgccacc gagcagcgcg agcccaccaa ggccttcctg tggatctatg
      841 tcctggcgta tttcggcggc tggctgggca tcctcggccc ggccatcctc gccctctaca
      901 cccgccttgc cgacctcttc ccgccggccg gccagggcac gaccgcggtc tccgcgctcg
      961 cggtggtctc cggcgtcggg gccgtggcgg ccaccctggc caaccccttc atcgggcgcc
     1021 tgagtgaccg caccacctcg cgcttcggga tgcggacccc ctggctgatc atcagctcgg
     1081 tgatcttcgt cctcggctcg ctcgtcgtgg ccttcggacc ggccaacatc gccgtcgtca
     1141 cgatcggctg gtcgctctcg caggtgggct tcaacggcat gctggccatc ttcaccgccg
     1201 tgcttcccga ccagatccct gagcggatga tgggccgggt gtcctcggtg ctgggcatcg
     1261 cgatgaacgt cgccagcctc gccgccgtct ggttggtcgg cttgttcgcc gcgggcacca
     1321 tcaccttcga cgacgccggc gtcatcaccg gaggcgaccc gtcgagcccc ctgcgcttca
     1381 tcgtccccgt ggccgtcgcc gtcgccacct cgatgatcct cgtcgtggcc ttgcgcccga
     1441 tcgaccggca catccagcgc tcccaggtgc cgccctacag cgtgggcgag ttcttcgggt
     1501 ccttcgtctt caacccccgc aagaaccccg acttcacctg ggcctgggtc agccggttca
     1561 tgttcatgct cggcatcgcc tacctgctcg tctaccaggc cccctactcc tccgcgcacc
     1621 tcaagttcga gggtgccgat ctcgaccggg tcgtcctgtg gggcaccgtc gtcaccgtcg
     1681 tgggcacggt gctcaccggc tacgtcgccg gccagttgtc cgaccggttc aagcgccgca
     1741 agccgttcgt catcggatcg gcgatggggt atgccgtgag cctggtcttc ctgtcgctcg
     1801 ccgcaccgga caactccggt ctgcccatgg cgctcgtcgg cctgttcctc ggcgggctgg
     1861 ctcagggtgt ctacttcggc atcgacctcg ccctggtgac cgatgtcctg cccgaccggg
     1921 aggccgacgc ggccaaggac ctcggggtgt tcaacatcgc ctcggccggc ccgcagttca
     1981 tcgcaccgtt catcgccccg gtgttcctcg gcctgaacct gctcggctcc ggcgccaacc
     2041 aggacaactt cgccgcgctg ttcgtcttcg ccgcgatctt ctcggcgatc ggtgccctgc
     2101 tcgtgctccc gatcaaggcc gtgcggtagg tcgacgatgt ccgcacccga agccccatcg
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgatcgacga actgctcggc
     2281 cggctcaccc tcgagcagaa ggtcgagctg atcaccggcg cgagcttctg gacgacggcc
     2341 gcctgcccgg agatcgggct gcgctcgctc accctgtcgg acggtccggc gggggtgcgc
     2401 ggccggttct ggtccgagca gcacccgtcg gctgcgctgc ccaacgcggt ctgcctcgcg
     2461 gcgaccttcg accccgagtt ggtcgagcgg gccggccgtc tcatcgggtc ggaagcgcgg
     2521 cgcaatggcg tcgacgtgct gcttggcccg acggtcaacc tgcaccgatc gccccacggc
     2581 gggcgcaact tcgaggcgtt cagcgaggac gccgtgctga ccgcacagac cggtggcgct
     2641 ctggttgacg gcatccagag ccagggtgtc gcagccaccg ccaagcactt catcggcaac
     2701 gactccgaga ccgagcggat gaccctcgat gcggtcatcg acgaggcgac gctgcgcagc
     2761 acctatgcgc tgcccttcga ggagctcgtc gtcgaccatg acgtggccgc ggtgatgagc
     2821 agttacaaca gcatcaacgg cacctcgggt acggagtcgc cgctgctgga atcgttgctg
     2881 cgggcggagt ggggattcgc ctcgctcgtc atctccgact ggggcgcggt tcgctcggtc
     2941 gaggccgctg ccgtggcacg cacggatctg gccatgccgg ggccggagtc accgtggagt
     3001 gagggcctgg ttgcggcggt gcgcgccggg cgcgtcgacg aggccgacgt cgatgtcaag
     3061 gtggcctccc tgctgggtct ggccgcacgg ctcggtgcgc tgagcggcat acctcctcgg
     3121 caggacccga ccgcgctgga cgatccccac gacccgtcgt ccccggccgt ccgggatctc
     3181 ctggcgacga tcgccgcccg cggcgccgtc ctgctgcgca acgagggcgc gaccctgccc
     3241 ttggagccga gtccggccag cattgccgtg atcggcccgg cggccacgcg gctgcgggtc
     3301 ggcggcgggg gcagcgcgac cgtggtgccg ccgcatgtcg tctccgtcgt gacggggatc
     3361 gaggcggcct tcccggacgc cctggtccac gccgtcgagg gcgtcaccca acatcgcctg
     3421 ttgcggccga tcggggatcg atcgaccaac gacctggggg cagcgggata cgacgtggag
     3481 ctcctcgacc tggaaggaac ggtccttgcc gctcaggcac ggaccggggg cgagctcatc
     3541 tacggcatgg ggttccccga tggcgtcgac ggcgatctgg tcactcgtat ccgggtgacc
     3601 atgacgctgc acgagacggg gccgcgaagg atcggggtcg ccggaatcgg ggacgccgag
     3661 atctggatcg agggtcagcg cgttgccgca cagaccctgc gcaacgaaca cgacgatgag
     3721 attgccgggc tgtcgcgccc accgcagctc gtcttggatg tctcactcac cggtggtgag
     3781 cgcgtcgtca tcgagttcgc tcccgcccct gacaccatcg ccgcgctgcg gatcggcctc
     3841 gaaggcccgg cgcgatccac ggcggacctg ttggaggaag cggtggcggc ggcccgagcc
     3901 gcggacgtgg ccgtggtcgt cgtcggcacg accgaggccg aggagtccga aggtttcgac
     3961 cgggagagcc tcagcctcgg cgccgacatc gacgccctgg tcgaggccgt ggccgcggtg
     4021 aacccccgca ccgtcgtcgt cctcaacgtc ggcgcgcccg tcctgctccc ttggcgggag
     4081 cgggtggccg cgatcctgtt ggcccacttc ccggggcagg agggcggcac cgccatcggt
     4141 cggattcttg cgggcgccga ggagccgggc ggtcggttgc cgagcagttg gccgcgcacc
     4201 gccgactccc gggttccgtc ggtgacgccg gtggccggca ccctcgccta ccaggagggt
     4261 gaggcgttcg gctaccgcga cccggagggc gacttcgcgt tcgtcttcgg tgacgggctg
     4321 ggcttcacga cctggagccg gtcgccctcc agcgcagcgc tgttgggtga cgggtcactg
     4381 cgggtcagcg cgtccgtgga gaacacgggc tcgcggcgcg ggcgtcagcc ggtcctcgtg
     4441 ttcgtcgctc ccgccggctc gccggaggcg ctgcggttcg cggggtcgac gatggtcgac
     4501 ctggcaccgg ggaccagcac ggtggccgag gtcgtggtgc ccagccgccg gcttcagcga
     4561 ctgctcccga gccccgaggc gggcctgatc gtcagcctgg gcttctcggt ccgcgaccgc
     4621 gaacccgccc g
//
LOCUS       DAORPW010000185         4615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5525_length_4615_cov_910.875877, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000185 DAORPW010000000
VERSION     DAORPW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5525_length_4615_cov_910.875877"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /gene="dnaN"
                     /locus_tag="PLB86_11380"
     CDS             complement(<1..496)
                     /gene="dnaN"
                     /locus_tag="PLB86_11380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRC65785.1"
                     /translation="MKFRVEREVLADAVAWVARGLPNRPPVPVLSGVLIEADRAGTVT
                     LSAFDYEVSAKVTVAADVDEGGTVLVTGRLLSEISRALPARPVDVSTEGNKVTVTCGT
                     SRFSLLMMQADDYPTLPAAPQPSGTIAGDVFTQAVHQVAIAADRGDTLPILTGVRMQI
                     EGDKV"
     gene            complement(1007..2479)
                     /gene="dnaA"
                     /locus_tag="PLB86_11385"
     CDS             complement(1007..2479)
                     /gene="dnaA"
                     /locus_tag="PLB86_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRC65786.1"
                     /translation="MTGGDVDLTDLWQRTLVALERTGVSPSERAFVGFARLMAVVEQT
                     ALIAVPNDYTKKVLESDVREHLVATLSGALGRDIGLAVTVDPTLEHATPSSNGVDPAN
                     GFDTANGDERHESPDAARMERFHPEGSGRHTPGVIAFPRRDGDQDRSRLNPKYTFDTF
                     VAGSSNRFAHAAANAVSEAPAKAYNPLFIYGSSGLGKTHLLHAIGHYVRQIYPGFRVR
                     YVNSEEFTNEFINSIRDDRASTFQSLYRDVDVLLIDDIQFLAGKVQTQEEFFHTFNAL
                     HNNSKQVVITSDLPPKQLSGFEERLRSRFEWGLITDVQAPDLETRIAILRKKTLQERM
                     SAPDEVLEYIATRITSNIRELEGALIRVTAFASLNRQPVDFTLAEVVLKDLVPTDQPV
                     QITIGTIQAETASYYALTVDDLCSSSRSRQLVTARQIAMYLCRELTELSLPKIGQAFG
                     GRDHTTVMHADRKIRELMSERRAIYNQVTELTARIRNHSR"
     gene            2942..3079
                     /gene="rpmH"
                     /locus_tag="PLB86_11390"
     CDS             2942..3079
                     /gene="rpmH"
                     /locus_tag="PLB86_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRC65787.1"
                     /translation="MSKRTFQPNNRRRAKTHGFRLRMRTRAGRAILAARRRKGRSELS
                     A"
     gene            3089..3445
                     /gene="rnpA"
                     /locus_tag="PLB86_11395"
     CDS             3089..3445
                     /gene="rnpA"
                     /locus_tag="PLB86_11395"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRC65788.1"
                     /translation="MLPARNRLRESGQFRAVLRGPGGSRAGGPLMVVHAAMTDPMGAR
                     APRVGFVVSGAVGGAVVRNRVKRRLRALAGTALGGIPAGCDLVVRAAPAAATASFAEL
                     QAELDRCLRRALAKVA"
     gene            3450..3752
                     /gene="yidD"
                     /locus_tag="PLB86_11400"
     CDS             3450..3752
                     /gene="yidD"
                     /locus_tag="PLB86_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRC65789.1"
                     /translation="MTLAELLGPGLRGPWTWRKAAAAPLLVLVRIYQLAISPLLPQRC
                     RFYPSCSAYAVTALTRFGPLRGGWLAARRLGRCHPWNPGGVDHVPEREACRTHAAT"
     gene            3844..>4615
                     /gene="yidC"
                     /locus_tag="PLB86_11405"
     CDS             3844..>4615
                     /gene="yidC"
                     /locus_tag="PLB86_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRC65790.1"
                     /translation="MGDFINLILSPLTIGEAWVMLAWHKVWTTIGIGGGWAWALSIVG
                     LTVVVRIVLIPLFVKQIHSSRRMQLIQPEMQKIQAKYKGKTDPESRQAMTQETMDLYK
                     KTGTNPFSSCLPILLQSPFFFALFTVLNNLKGIAEGTHPAIGPITPAVAHEIESSNIF
                     GAPFSSTFMGSTDTATKIVTVVLIILMSVSTFTTQRQLMRKNLPDSALDNPFMKQQTV
                     LLYLMPIFFALSGINFPIGVLLYWLTTNVWSMGQQFYVI"
BASE COUNT          702 a         1452 c         1626 g          835 t
ORIGIN      
        1 tgaccttgtc gccctcgatc tgcatccgga cgccggtgag gatcggcagg gtgtcacccc
       61 ggtcggccgc gatggcgacc tggtgcacgg cttgagtgaa cacgtcgccg gcgatggtgc
      121 cggagggctg cggcgcggcc ggcagggtgg ggtagtcgtc ggcctgcatc atcagcaggc
      181 tgaaccggct cgtcccgcag gtgaccgtga ccttgttgcc ctcggtcgag acgtcaaccg
      241 ggcgagcggg gagggcgcgc gagatctccg agagcaggcg cccggtgacc agcacggtgc
      301 cgccttcgtc aacgtcggcg gcgacggtga ccttggcgga cacctcgtag tcgaaggctg
      361 acagcgtgac cgtgccggcc cgatcggctt cgatcagcac gccggacagg acgggcaccg
      421 gaggtcggtt cggcagaccg cgagccaccc aggccacggc atcagcgagc acctcgcgct
      481 caacgcggaa cttcacgatc gtctccaccc tcgtgcttct tcgatccaga ccccggctca
      541 accggcagaa cggcgcgggc gcgcgcacca ccgccgtgtc ggggggaact ccgaccctag
      601 tcggttgggc gggaactgca agagggaagg gtcaccgcag ttgcgcacgc tcctgcctga
      661 tccccagcgt tgtcggggca atggagagaa ttggtggact cgtcgtcgtc ataggtgttg
      721 tggattctgt ggataaccct caagtccgca ggtcagcggc ccaagcggcc tgggtatgcc
      781 gcgtgcatga gtcggtgggt aactcaccga gcctgttgac gggcaccacg gacccacagc
      841 cagtgcactg tgagggtgtt gttgtccccc aaatggtgga cgttgtccac agctgtccac
      901 agccgagtgt ggacaactta ggggagggct cagggcagag gaagctgcag gggggagcgt
      961 gaggcgggag gagcgtgacg cgacggagcg tgccggagcg aatgggtcag cgcgagtggt
     1021 tgcggatgcg cgcggtgagc tcggtgacct ggttgtagat cgcccgacgt tcactcatca
     1081 gctcgcggat cttgcgatcg gcgtgcatga cggtcgtgtg gtcgcggccg ccgaaggcct
     1141 ggccgatctt gggcagcgac agctcggtga gctcgcggca caggtacatg gcgatctgcc
     1201 gggccgtcac caactgccgc gagcgcgaag aactgcacag gtcatcgacg gtgagggcgt
     1261 aatagctcgc cgtctcggcc tggatggtcc cgatggtgat ctgcaccggc tggtcggtgg
     1321 ggacgaggtc cttgaggacc acctcggcga gggtgaagtc gaccggctgc cggttgaggc
     1381 tggcgaacgc ggtgacccgg atgagggccc cttcgagctc gcggatgttc gaggtgatcc
     1441 gggtggcgat gtattccagc acctcgtccg gggcgctcat ccgctcctgg agggtctttt
     1501 tgcgcaggat cgcgatgcgg gtctcgaggt cgggtgcctg cacgtcggtg atgaggcccc
     1561 actcgaaccg actacgcagc cgctcctcga aaccactgag ttgcttgggc ggcaggtcac
     1621 tggtgatgac gacctgcttg ctgttgttgt gcagcgcatt gaaggtgtgg aagaactcct
     1681 cctgcgtctg caccttgccg gcgaggaact ggatgtcgtc gatgagcagc acgtcgacat
     1741 cgcggtagag gctctggaag gtgctcgcgc ggtcgtcacg gatcgagttg atgaactcgt
     1801 tcgtgaattc ttcggagttg acgtagcgca cccggaagcc gggatagatc tggcggacgt
     1861 agtggccgat tgcgtgcagc aggtgggtct tgcccagccc cgaggagccg tagatgaaca
     1921 acgggttgta ggccttggcc ggcgcttcgg acacggcgtt agccgcggca tgcgcgaacc
     1981 ggttgctcga cccggcgacg aaggtgtcga aggtgtactt cgggttgagc cggctgcggt
     2041 cctggtcacc gtcgcgtcgg gggaaggcga tcaccccagg ggtatgccgc ccgctcccct
     2101 cgggatggaa gcgttccatc cgcgcggcgt cggggctctc gtggcgctcg tcgccgttgg
     2161 cagtgtcgaa cccgttggcg gggtcgaccc cgttggagct gggggtggcg tgctcgaggg
     2221 tggggtcgac cgtgacggcc agcccgatgt cgcggccgag cgctccgctg agcgtcgcga
     2281 cgaggtgctc gcggacgtcc gactcgagca ccttcttggt gtagtcgttg ggcacggcga
     2341 tgagggcggt ctgttcgacg acggccatca ggcgggcgaa accgacgaag gcacgctcac
     2401 tgggggagac accggtgcgt tccagagcca cgagggtgcg ttgccacagg tcggtgagat
     2461 cgacgtctcc gcctgtcacg tgtggctccc ctccttgccg acgccgggcc cgttgggtcg
     2521 ggcctccaga cgttcgacat tacccgtcat ccacagctct atccacaggg tggggacaaa
     2581 ccaccgatgg gggaaggcct cgcagccgga agcatcgtct gtccgggggc tgcgtggaga
     2641 gattacaccc cttgatgggc gtgtcgctcc cctaaaccgg aatgacgagg ggggagaatg
     2701 ctgggttttc gttgctggtc gaggtcccgg gtttgaccct gcccgccgac gggccgtacc
     2761 ctgatctgcg gcccatgtgt cgactctgac tccggccgct ttcgcgcgcc cgtgcttgtc
     2821 accgcgggct tccgattgcg gccggcggac ggggtgacgc ggcatacggg cgccccgcag
     2881 tccaccgcag tccgcccgca ggctacccgc cctgggcctg ccgatcgacg gagacagacc
     2941 cgtgagcaag cgcaccttcc agccgaacaa ccgccgtcgc gccaagacgc acggcttccg
     3001 cctgcggatg cggacgcgcg ccggtcgcgc gatcctcgcc gctcgccgtc gcaagggccg
     3061 ctccgagctg tccgcctgag gcggatatgt gctcccggcc cgcaatcggc tgcgtgagtc
     3121 gggccagttc cgtgccgtcc tccgcggccc tggtgggtcg cgagccggcg gaccgttgat
     3181 ggtggttcac gcagcgatga ccgatccgat gggtgctcgc gccccccggg tcggtttcgt
     3241 cgtgtccggc gccgtcggag gcgccgtcgt gcgtaatcgg gtcaagcgcc ggttgcgcgc
     3301 gctggccggg actgcgctgg gcggcatacc ggccgggtgt gacctggtcg tgcgggcggc
     3361 tccggccgcg gcgacagcgt cgttcgccga gttgcaggcc gagttggatc ggtgcctgcg
     3421 tcgcgccctg gccaaggtgg cgtgagctga tgacgctggc agagctgctc gggcccggat
     3481 tgcgcggtcc gtggacgtgg cgcaaggcgg ctgcggcacc cctgctcgtg ctcgttcgga
     3541 tttaccaact ggcgatctcg ccgctgttgc cgcaacggtg ccgcttttac ccctcctgct
     3601 cggcgtatgc cgtcaccgcc ctcacgcggt tcggtccgct gcgcggtggc tggctggctg
     3661 cgcgccggct gggccgctgc cacccgtgga accctggcgg cgttgaccac gttcccgagc
     3721 gggaagcctg ccgcactcac gcggcgacct agaccacctg gtccgcgagc ggcatacctg
     3781 gactgctgac cggaccgaac catccggcga gatgagagtc tcgcctttcg agcaaggacg
     3841 acagtgggcg acttcatcaa cctcatcctc tcgccgctga ccatcggcga ggcctgggtg
     3901 atgcttgcct ggcacaaggt ctggacgacg atcggcatcg gtggtggctg ggcctgggcc
     3961 ttgtcgatcg tcgggctgac ggtcgtcgtg cgaatcgtgc tcatcccgct cttcgtcaag
     4021 cagatccact cgtcccggcg gatgcagttg atccagccgg agatgcagaa gatccaggcg
     4081 aagtacaagg gcaagaccga tcccgagtct cggcaggcga tgactcagga gacgatggat
     4141 ctctacaaga agaccgggac caacccgttc tcctcgtgtc tgccgatcct gctgcagtcg
     4201 ccgttcttct tcgcgctgtt cacggtgctc aacaacctca agggcatcgc ggaggggacc
     4261 cacccggcga ttgggccgat cacgccagcg gtggcccatg agatcgagtc gtcgaacatc
     4321 ttcggggcgc cgttctcctc gacctttatg gggtcgaccg acacggccac gaagatcgtc
     4381 acggtcgtgc tcatcatcct catgtcggtg tcgacgttca cgacgcagcg gcagttgatg
     4441 cgcaagaacc tgcccgactc ggcgctggac aacccgttca tgaagcagca gacggtgttg
     4501 ctctacctca tgccgatctt cttcgccctc tcgggcatca acttcccgat cggcgttctc
     4561 ctctactggc tgacgaccaa cgtgtggtcg atgggccagc agttctacgt catcc
//
LOCUS       DAORPW010000186         4611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5537_length_4611_cov_721.808824, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000186 DAORPW010000000
VERSION     DAORPW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5537_length_4611_cov_721.808824"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..570
                     /locus_tag="PLB86_11410"
     CDS             61..570
                     /locus_tag="PLB86_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRC65791.1"
                     /translation="MSNAASPGGTSEPNATGSGWLLRRIRDQQVAFLLVGATNTAVGF
                     LVFVGFDRLYAAVAPAWGSVLHNTVTLACSHVVSVIIAFVLYRTLVFRVTGHLWRDLA
                     RFESVYLVSLAANWLLLNLLTVVIGLSVIVAQALVVAVIAMVSYFGHKHFSFRRPDPA
                     APDPPGGAE"
     gene            567..1421
                     /locus_tag="PLB86_11415"
     CDS             567..1421
                     /locus_tag="PLB86_11415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRC65792.1"
                     /translation="MTASQSSPRVSVVVPTFHNAQFVEATIESILAQTFTDFELLVSD
                     HSSTDGTWDRLQRFAADPRVRLWQTPTGGGAPANWAAVTDAARGEFVKLVCGDDLLYP
                     TCLREQVDAMDAHPSATLVCARRDLIDAQGGIVMGGRGMSRIRGFHSGRAAIRRTVAL
                     GTNIFGEPASVLMRREALVGAGGWWPDFPYVIDEATYALVLLRGDLYAVPHSLAAFRI
                     SAGQWSVDLADQQAAQVIAFHEELDRRVPGLLTRADLARGKAMARGLSTARRLVYLAM
                     RRRMTTSE"
     gene            1531..2340
                     /locus_tag="PLB86_11420"
     CDS             1531..2340
                     /locus_tag="PLB86_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRC65793.1"
                     /translation="MDKVVSSAAAALAGIESGMSLAVGGFGLCGIPSVLIQALHDSGV
                     DHLEVISNNCGVDDWGLGILLRDRRIRRMISSYVGENKEFERQYLSGELEVELTPQGT
                     LAERMRAGGSGIPAFFTMTGVGTQVAEGGMPWRYAADGTVAVASPAKEVREFDWMGRR
                     REFVLEQAIVADYALVRAARGDRHGNLVFKESTRNFNPLAAMAGRVTIAEVEQLVEPG
                     ELDPDHIHLPGIFVQRVLALSPEQASVKRIERRTVRPKPAAPSAATTEQEA"
     gene            2344..2985
                     /locus_tag="PLB86_11425"
     CDS             2344..2985
                     /locus_tag="PLB86_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HRC65794.1"
                     /translation="MALTREEMAARAARELHDGDYVNLGIGLPTLVPNYVPDDVEIVL
                     QSENGILGVGAYPFEGEEDPDLINAGKETVTIRRGASFFDSATSFGMIRGGKVNAAIL
                     GAMQVSAAGDIANWMIPGKMVKGMGGAMDLVHGAQHVIVLMEHVARDGAHKIVQECSL
                     PYTGKRVVHRIITDLAVMDVTPDGLVLRELAPGVTLDEVRAATGPDFRVELDA"
     gene            complement(2991..3704)
                     /locus_tag="PLB86_11430"
     CDS             complement(2991..3704)
                     /locus_tag="PLB86_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03089 family protein"
                     /protein_id="HRC65795.1"
                     /translation="MPTRSSMPADVLAAMLRLDPGRPRITCYDDASGERIELSAKTFA
                     NWVAKAANLLQDSGDVTVGNTVGLDLPAHWRTAYWAMAAWSVGCIVVVGPEARDADVV
                     VTVSPDRAAECVSDGRYAVLVTLAALARGNAQTPQDVLDEAKELAGYGDRFDPWDTPE
                     ADEPALVGDGDDAAYAQVVVRRDWPERPRVLVSGDRLGSALRAFLSAWAADGSVLLVL
                     NPAGDQDRRRAIESVTATA"
     gene            3737..>4611
                     /locus_tag="PLB86_11435"
     CDS             3737..>4611
                     /locus_tag="PLB86_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRC65796.1"
                     /translation="MAEPAVVRQWRSARPVQLAAILAPFRRGGGDPTFRVGPDGSVVR
                     GIQTPDGPATLELDCAPKESEISAQAWGPGAAWAVEQAPALVGEGDDPSGFVGLHPPV
                     VDAIRRFPGWRVPRSGLVLDALVPAIIEQKVTGKEAFGGYHRLVRIHGDVAPGPWGAA
                     GLRVPPDAAGWRAIPSWEFLRASVDASRSDTIQRVCRVAGSLERLTSGSTEAGRAGLR
                     SVSGVGVWTAAEVAQRAWGDADAVSFGDYHVARTIGWTLVGQELDDAGLATLLEPYAG
                     HRFRVQRLLELAGHQR"
BASE COUNT          660 a         1589 c         1635 g          727 t
ORIGIN      
        1 atcacctcgc cggcggcctg ggcgcggacc ttcgggctgg acgacccggc gtgacgtgtg
       61 atgagcaacg cggcgagtcc aggcggcacg agcgaaccga acgcaacagg ttcaggctgg
      121 ctgctgcgcc gcattcgtga ccagcaggtg gcgttcctcc tcgtgggcgc caccaacacc
      181 gccgtcggct tcctcgtctt cgtcggtttc gaccggttgt atgccgccgt cgcccctgcc
      241 tggggcagcg tccttcacaa caccgtcacg ctggcctgct cccacgtggt gtcggtcatc
      301 atcgccttcg tgctctaccg caccctcgtg ttccgcgtca ccgggcacct ctggcgagac
      361 ctggcccggt tcgagagcgt ctatctggtg tcgctcgccg ccaactggct gctgctcaac
      421 ctcctcaccg tcgtcatcgg cctctcggtg atcgttgcgc aggccctggt cgtcgccgtc
      481 atcgccatgg tgagctactt cgggcacaaa cacttctcgt tccgccgccc tgaccccgcg
      541 gcccccgacc cgccgggagg tgcggagtga cggcctcaca gtcgagcccg cgggtctcgg
      601 tcgtcgtccc cacgttccac aacgcgcagt tcgtggaggc gacgatcgag tcgatcctcg
      661 cccagacctt caccgacttc gagttgttgg tgagcgatca cagctcgacg gacggcacct
      721 gggatcgact gcagcggttc gccgccgacc ctcgggtgcg gctgtggcag acgccgaccg
      781 gaggcggtgc gccagccaac tgggctgccg tcaccgacgc cgcacggggg gagttcgtca
      841 agctcgtctg cggcgacgac ctgctgtatc ccacctgtct gcgtgagcag gtcgacgcga
      901 tggacgccca cccgagcgcc accctcgtgt gcgcgcgacg cgacctcatc gacgcgcagg
      961 gcgggatcgt catggggggc cgcgggatga gccgcattcg tgggttccac agtgggcgag
     1021 ccgcgatccg gcgcacggtc gccctcggca cgaacatctt cggcgagcca gcctcggtcc
     1081 tcatgcgccg cgaggccctc gtcggcgcgg gcggctggtg gccagacttc ccctacgtca
     1141 tcgacgaggc cacctacgcg ctggtcctgc tgcgcgggga cttgtatgcc gtgccccaca
     1201 gcctggctgc gttccggatc agcgcagggc agtggagtgt cgacctggcc gatcaacagg
     1261 ctgcccaggt gatcgcgttc cacgaagagt tggaccgacg ggtgccgggt ctgctcaccc
     1321 gtgccgacct ggcccggggc aaggcgatgg ccaggggact gagcaccgcg aggcggctgg
     1381 tctatctcgc gatgcgacgc cggatgacga cgagcgagta gccgaacgcg cgcgctggcg
     1441 cggttccacc gcgtgaactc cacgcaccga caacgtgtgc tggggctccc ggcctgattc
     1501 cgctgccgac gcgcggctag agtcgggggc atggacaaag tcgtctcttc cgctgccgca
     1561 gccctcgcag gcatcgagtc ggggatgtcg ctcgccgttg gcgggttcgg cctctgcggg
     1621 atcccctcgg tgctcatcca agccctgcac gacagcggcg tcgaccacct ggaggtcatc
     1681 tccaacaact gcggagtcga cgactggggg ttgggaatcc tcctgcggga caggcggatt
     1741 cggcgcatga tctcctccta cgtgggtgag aacaaggagt tcgagcggca atacctctcc
     1801 ggcgagcttg aggtcgagct caccccccag ggcacgctgg ccgagcggat gcgagccggg
     1861 gggagcggca taccggcgtt cttcacgatg accggggtcg ggacgcaggt cgctgagggg
     1921 ggcatgccct ggaggtatgc cgcggacggc accgtcgccg tcgcatcgcc ggccaaggag
     1981 gtccgtgagt tcgactggat ggggcgccga cgcgagttcg tcctcgagca agccatcgtc
     2041 gccgattacg ccctcgtccg agccgcacgc ggcgaccggc acggcaacct tgtcttcaag
     2101 gagtcgaccc gcaacttcaa cccgctggct gccatggccg gccgggtgac cattgccgag
     2161 gtcgagcagc tcgtcgagcc gggggagttg gaccccgacc acatccacct gcccgggatc
     2221 ttcgtccaac gggtgctggc cctgagcccc gagcaggcct cggtcaagcg gatcgaacgc
     2281 cgcaccgtgc gacccaagcc cgccgcgccg agcgcagcga cgaccgagca ggaggcctga
     2341 gccatggcgc tcacccgtga ggagatggcg gcccgagccg cccgcgagtt gcacgacggc
     2401 gactacgtca acctcggcat cggcctgccg accctcgtgc ccaactacgt ccccgatgac
     2461 gtcgagatcg tcctgcagtc cgagaacggc atcctcggcg tgggcgccta tcccttcgag
     2521 ggcgaggagg accccgacct catcaacgcc ggtaaggaga ccgtgacgat ccggcgcggg
     2581 gcgtcattct tcgactccgc gacgagcttc gggatgatcc gcggcggcaa ggtcaacgcg
     2641 gcgatcctcg gcgccatgca ggtgtcggcg gctggcgaca tcgccaactg gatgatcccc
     2701 ggcaagatgg tcaaggggat gggcggggcg atggacctcg tccacggcgc ccagcacgtc
     2761 atcgtcctca tggagcacgt cgctcgcgac ggtgcccaca agatcgtgca ggagtgctcg
     2821 ctgccctaca ccggcaagcg ggtcgtccac cggatcatca ccgacctcgc ggtcatggac
     2881 gtcacccccg acgggcttgt cctgcgcgag ctcgcgcccg gggtcactct cgacgaggtg
     2941 cgcgctgcca ccggccccga cttccgggtc gagctcgacg cctgaccgga tcaggctgtg
     3001 gcagtgacgc tttcgattgc ccgtcgacgg tcctgatcgc ccgccgggtt gagcacgagc
     3061 agcaccgatc cgtcggcggc ccaggcgctg aggaaggctc gcagcgcgga cccgagccgg
     3121 tcgccgctga ccaggacccg tggccgctct ggccagtccc gacggacgac gacctgcgcg
     3181 tatgccgcgt cgtcaccgtc gccgaccagg gccggctcgt ccgcctccgg ggtgtcccac
     3241 gggtcgaacc ggtcgccata accggccagt tccttggcct cgtcgaggac gtcctgcggc
     3301 gtctgcgcgt tgccgcgggc tagcgccgcg agggtgacga gcacggcata ccggccgtcg
     3361 gagacgcact cggcggcacg gtcgggggag accgtgacga ccacgtcggc gtcccgggcc
     3421 tctggtccga cgacgacgat gcagcccacc gaccaggccg ccatcgccca gtaggccgtg
     3481 cgccaatggg cgggcaggtc caggccgacg gtgttgccga cggtgacgtc gccgctgtcc
     3541 tgcaagaggt tggcggcctt ggcgacccag ttggcaaagg tcttggcgga caactcgatc
     3601 cgctcaccgc tcgcatcgtc gtaacacgtg atgcgagggc gccccgggtc taagcgcaac
     3661 atcgcggcga ggacgtcggc gggcatggag gatcgggtcg gcacgagcgt cagcctacgt
     3721 cggtagagtc cgcggcatgg cggagcccgc agtcgtgcgt cagtggcgca gcgcccggcc
     3781 ggtgcaactc gcggcgatcc tcgcgccgtt ccgtcgtggt ggtggcgacc cgacgttccg
     3841 ggtggggccg gacgggtcgg tggtgcgcgg catacagacc cctgatgggc cggccaccct
     3901 cgaacttgat tgtgccccaa aggagtccga gatctcggcg caagcctggg ggcctggcgc
     3961 ggcctgggcg gtggagcagg cgccggcgtt ggtcggtgag ggcgatgacc cgagcgggtt
     4021 cgtcgggttg cacccgcctg tcgtggatgc gatccggcgc ttcccggggt ggcgggtgcc
     4081 gcgcagcggg ttggtcctcg acgccctcgt gccggccatc atcgagcaga aggtgacggg
     4141 caaggaggcg ttcggcggct accaccggct ggtccggatc catggcgatg tggcgccagg
     4201 gccgtggggc gccgctgggc tgcgggtccc accggatgct gcgggctggc gggcgatccc
     4261 gtcgtgggag ttcctacgcg cgtcggttga cgccagtcgc tcggacacga tccagcgggt
     4321 atgccgtgtc gccggctccc tcgagcggct cacctcgggg tcgaccgagg cgggccgggc
     4381 ggggctgcga tcggtttccg gggtcggggt gtggacggct gccgaggtgg cccaacgggc
     4441 ctggggcgat gccgacgcgg tgagcttcgg ggactatcac gtggctcgca ccatcggctg
     4501 gaccctggtg gggcaggagc tcgatgacgc cggtctggcc accctcctcg agccgtatgc
     4561 cgggcatcgc ttccgggtcc agcgactgct ggagttggcc ggccaccaac g
//
LOCUS       DAORPW010000187         4586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5593_length_4586_cov_848.632311, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000187 DAORPW010000000
VERSION     DAORPW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5593_length_4586_cov_848.632311"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLB86_11440"
     CDS             complement(<1..416)
                     /locus_tag="PLB86_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRC65797.1"
                     /translation="MSRRGDLLEFAVLGLLHESPMHGYELRKRLNTALGAFRALSYGS
                     LYPCLRELLGKGWIFESSSVEGDALSGSRRARIVYELTADGKERFQDMAETAGPTAWD
                     DEEFDVHFAFFARTKADVRLRILEGRRSRLEERLAN"
     gene            743..2944
                     /locus_tag="PLB86_11445"
     CDS             743..2944
                     /locus_tag="PLB86_11445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRC65798.1"
                     /translation="MSHDHTQPDPRATRSGRGNGAGTSAGTGSSGSKAKRSWWRAPRR
                     TWIKRVVWTVVGFLALGASAVAVAFATIQVPDVANGLAEAQTSIVYYDDGKTEMARIA
                     ELDREPLPLASIPKHLQHAVIAAEDRDFYTNAGISPTGIARSVWQAVSGSDVQGGGST
                     ITQQYVKNYFLTQDRTLDRKLREWVISIKIDRDMTKDQILEGYLNTIYFGRGAYGVKT
                     AARAYFGKDVSALTVQESAVLASVMNAPSLFDPALGAKQQTNLTKRVDYVLDGMVTMG
                     WLSAADRAPLTGLPTIAAKAPSRALSGPTGYIVAAVRRELTTKLQLSDDDIDRGGLRI
                     TTTISAPAQQAAQDAVAKDFPTTGDVKDVYAGLVAVRPGDGAIVAMYGGADYQARQFS
                     SATDAVIQGGSIFKAFGLIGALQQGISTKTQFDGNSPLRDPALGNTPVRNVGGRSYGT
                     VDLRKATASSVNTAFVRLNIKMGPSVTKTAAIAAGIPESTPGLGDDPTNVLGTASPHV
                     LDVANAYATIAAQGRRATPYLIKTVTSNAIDVTYQAVPNVVDAFSRDVAADVIDAMQQ
                     VTAAGGTGSRASAVGRPVAGKTGTSEEAKSVWFSGFTPQLSASVAMFKDVNGVPQPLT
                     DIGGLDGLTGGSYPLSIWIDFMKGALAGQDKVDFPKRVGIGDDKVHTAAPTPTAPATS
                     VPTSTPDPTNSPKPEPTPSPTNSSPTSIPPRPTNTGGPTNANKPTTPPGQG"
     gene            3009..4415
                     /locus_tag="PLB86_11450"
     CDS             3009..4415
                     /locus_tag="PLB86_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HRC65799.1"
                     /translation="MTAADRVVVPSREDPVARSASVVAGGPLGRYAVPLARGWRRLAA
                     GLVAASCVPMAASVALRGYCIDAGWNSPDQFFHMCYSDLPATFGDQNLGAGLGAFLAG
                     GVGAPTPVQPPLTALLLAFTGGLVPDGSAQARARFYFGIWAVLIALLLAVTVWFTAAA
                     VRRMPMRAAHVALSPVVALTAVVSPDLLGVALASGALWAWSRSRPVISGVLFGLAISA
                     RSYPVLLLVAIALVAVRAGRGRTVLPVAGAAIVTFAAVVALVALGNPGGAMRAYSTWV
                     GAEAGFGSPWVLGQLARPDGAQSLGFSLPEPLSPSALTLLAAAGWLVAILVGAWLAWS
                     GERRPGVAEVSLVMVAIVLVTGKSFPVQASIWLVPLVALVGIQWRDHLIWSGAEALHF
                     GAVWLYLAGSSVADRGLPSGWYLLLLLVRLLAIGWLVVQVWWQARDRMPHVVSPRETD
                     PLAGPLRDAPDALIVRVG"
BASE COUNT          667 a         1669 c         1537 g          713 t
ORIGIN      
        1 atgttggcca ggcgctcctc cagccgcgaa cggcgcccct cgaggatgcg cagccggaca
       61 tcggccttgg tccgggcgaa gaaggcgaag tggacgtcga actcctcgtc gtcccacgcc
      121 gtcggcccgg ccgtctcggc catgtcctgg aagcgttcct tgccgtcggc cgtcagctcg
      181 tagacgatcc gcgcccgccg gctgcccgag agcgcatcgc cctcgacact cgacgactcg
      241 aagatccagc ccttgccgag cagctcgcgc agacacggat atagcgaccc ataggacaac
      301 gcccggaacg cacccagagc cgtgttgagt cgcttgcgca gctcgtagcc gtgcatcggg
      361 ctctcatgga gcaacccgag gacggcgaac tccagcagat caccacgtcg agacaccgtc
      421 actcacctcc gcctctggtc cggtccgttg atccggtatg ccgtgtgcct gggtccgcgt
      481 cggacccggg ctgtcgttcg tcgcaccttc ggatgggtac gcgcgtgcgt cgatgaccag
      541 cagactctag caccagatat atcgaaccga tacatcatcc cgatgtgtcg cgtgtcgcgg
      601 cggggtgccg acgggcagcc ggtggccagt ttgggtgtgt gcgattcggt agctcacagc
      661 ccgtccgctc atactggatc tcctgacggg gaatcgaccg gaaccctcca acgggcgacg
      721 acggcaggaa aggcactctc gcgtgagcca cgatcacacc cagccggacc cgcgggccac
      781 ccgctccggc aggggcaacg gcgcaggcac cagcgcaggc accggcagct ccggcagcaa
      841 ggccaagcgc agttggtggc gcgctccccg acggacctgg atcaagcggg tcgtctggac
      901 cgtcgtcggc ttcctcgccc tcggcgccag cgcggtcgct gtggccttcg ccaccatcca
      961 ggtgcccgac gtcgccaacg ggctggccga ggcgcagacc tcgatcgtct actacgacga
     1021 cggcaagacc gagatggccc ggatcgccga gctcgaccgc gagcctcttc ctctggcgag
     1081 catccccaag catctgcagc acgcggtcat cgccgccgag gaccgcgact tctacaccaa
     1141 cgcggggatc tcacccaccg gcatcgcccg ctccgtctgg caggccgtca gcggcagcga
     1201 cgtccagggt ggcgggtcga cgatcaccca gcaatacgtg aagaactact tcctcaccca
     1261 ggaccgcacc ctcgaccgca agcttcgcga gtgggtcatc tcgatcaaga tcgaccggga
     1321 tatgaccaag gaccagatcc tcgagggcta cctcaacacc atctacttcg ggcgcggcgc
     1381 ctatggcgtc aagaccgccg cccgggccta cttcggcaag gacgtcagcg cgctcacggt
     1441 ccaggagtcg gccgtcctgg ccagcgtcat gaacgccccg agcctcttcg acccggccct
     1501 cggtgccaag cagcagacca acctcaccaa gcgggtcgac tacgtcctgg acggcatggt
     1561 gacgatgggg tggctctcgg ccgccgaccg cgcgccgctc accggcctgc cgacgatcgc
     1621 cgccaaggcc ccgtcgcggg ccctctccgg tccgaccggc tacatcgtcg ccgcggtccg
     1681 ccgcgagctg accaccaagc tgcaactgtc cgacgacgac atcgaccgcg gcggcctgcg
     1741 gatcacgacg acgatcagtg cccccgcgca gcaggccgca caggacgccg tcgccaaaga
     1801 cttccccacg accggggacg tcaaggatgt gtatgccggg ctggtcgccg tccgccctgg
     1861 cgacggcgcc atcgtcgcga tgtatggcgg tgccgactac caggcccgtc agttctcctc
     1921 ggcgaccgac gcggtgatcc agggcggctc gatcttcaag gcgttcgggc tcatcggcgc
     1981 cctgcagcag ggcatctcca ccaagaccca gttcgacggc aacagcccgc tgcgcgaccc
     2041 cgccctaggc aacaccccgg tccgcaacgt cggcggccgg tcctacggca ccgtcgacct
     2101 gcgcaaggca accgcctcgt cggtcaacac ggcgttcgtt cgactcaaca tcaagatggg
     2161 gccgtcggtg acgaagacgg cggcgatcgc ggccggcata ccggagagca ctccggggct
     2221 gggagatgac ccgaccaatg tccttgggac ggcctcgccg cacgtgctcg acgtcgccaa
     2281 cgcctacgcc acgatcgcgg cgcaaggccg ccgagcgacg ccgtacctca tcaagacggt
     2341 gacctccaac gccatcgacg tcacctacca ggcggtcccc aacgtcgtcg acgcgttctc
     2401 ccgcgacgtc gccgccgacg tcatcgacgc catgcagcag gtgaccgcgg ccggcggcac
     2461 ggggtcgcgc gcgagcgcgg tcggccgtcc ggtggcgggc aagaccggca cctccgagga
     2521 ggcgaagtcg gtgtggttca gcggcttcac cccccagctg tcggcatcgg tcgcgatgtt
     2581 caaggacgtc aacggggtgc cccaaccgct caccgacatc ggcggtctcg acgggctgac
     2641 cggcggctcc taccccttgt cgatctggat cgacttcatg aagggcgcgc tggcgggtca
     2701 ggacaaggtc gacttcccca agcgcgtcgg catcggcgac gacaaggtcc acacggccgc
     2761 gccgaccccg acggcccctg cgacgtcggt gcccacgagc acgcccgacc ccaccaactc
     2821 gccgaaaccc gagcccaccc cgtcgccgac caattcgtcg ccgacgagca tcccgccgcg
     2881 gccgaccaac accggcggcc cgaccaacgc caacaaaccg acaacgccac ccggtcaggg
     2941 gtagcgcccc gcaccgacaa accggcgcgc aggcggcata cctcaggcgg gcggcatacc
     3001 ctgtgggcgt gacagctgcg gaccgcgtcg tcgtcccctc ccgggaggac cccgttgcgc
     3061 gctcggcgag cgtcgtggcg gggggaccgc ttggccggta tgccgtcccg ctggctcgcg
     3121 ggtggcgccg cctggccgcc gggttggtgg ccgcgtcgtg cgtgccgatg gcggcgtcgg
     3181 tggcgctgcg cggctactgc atcgatgcgg ggtggaacag ccccgaccag ttcttccaca
     3241 tgtgttactc cgaccttccg gcgaccttcg gcgaccagaa cctcggcgcc ggcctcgggg
     3301 ccttcctcgc tggcggcgtg ggcgcgccga ctccggtgca gccgccgctg accgcgctgc
     3361 tgctggcctt caccggcggc ctcgtgcccg acgggtcggc gcaggctcgg gcccggttct
     3421 acttcggcat ctgggcggtg ctcatcgccc tgctcctcgc cgtcaccgtc tggttcacgg
     3481 cggcagcggt ccggcgtatg ccgatgcgcg cggctcacgt cgctctgtca ccggtcgtcg
     3541 cgctcaccgc ggtcgtctcc ccggacctgc tcggtgttgc cctcgcgtcg ggggccctgt
     3601 gggcctggtc gcgctcgcgg ccggtgatca gcggagtgct gttcggcctg gcgatctcgg
     3661 cgcgttccta tcccgtcctg ttgctcgtgg cgatcgcgct ggtcgcggtg cgggcggggc
     3721 gggggcggac cgtcctgccg gtcgcgggag cagccatcgt cacgtttgca gcggtggtgg
     3781 cgttggtcgc cctgggtaat cccggcggag cgatgcgggc ctattcgacg tgggtcgggg
     3841 ccgaggcggg gttcggctcg ccgtgggttc tcgggcagtt ggcccggccc gacggcgctc
     3901 agtcgttggg tttcagcctg cccgaaccgt tgtccccgag cgccctcacg ctgttggcgg
     3961 ccgccgggtg gctcgtcgca atccttgtcg gggcctggtt ggcctggagc ggtgagcgtc
     4021 ggccaggggt cgccgaggtg tcgctcgtca tggtggcgat cgtcctcgtg acggggaagt
     4081 ccttccccgt gcaggcgtcg atctggctcg tccccctcgt cgccctcgtc gggatccagt
     4141 ggcgcgacca cctcatctgg tccggggccg aggccctgca tttcggggcg gtctggctct
     4201 atctcgctgg gagttcggtg gccgaccgcg ggctgccctc ggggtggtac ctgctgctcc
     4261 tgctggtgcg cctgttggcg atcggatggc tcgtggtgca ggtctggtgg caggcccgcg
     4321 accggatgcc gcacgtggtg tcgccccggg agaccgatcc gttggccggg ccgctgcgcg
     4381 acgccccaga cgccttgatc gtccgcgtcg gctgaaacga gcgcgattag gtccccctgc
     4441 gggggtgccg taacctagga cggttcgcct gcggcatacc cctgtggaaa acttccggcc
     4501 ccctgtggcg cggtgaggca ggatcggtgg tcgaggcgcg gacacatgca accctcctgt
     4561 cacggagaga ccgtgaccgc gaagac
//
LOCUS       DAORPW010000188         4529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5718_length_4529_cov_737.551632, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000188 DAORPW010000000
VERSION     DAORPW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5718_length_4529_cov_737.551632"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="PLB86_11455"
     CDS             complement(<1..1418)
                     /locus_tag="PLB86_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HRC65800.1"
                     /translation="MTDATSSATPESAAPQPLSSAPTHTPSATSANPAGAAPDGKAAE
                     PEPTDELVTTRHTVTLSDGQVLDYSATTGRLVLREEVYEDGVFGGHPAKAQVSITSYV
                     REGLSGRERAARPVTFAFNGGPGSSSVWLHLGLLGPRRVVMGDVGALLPPPYALADNA
                     ETLLAESDLVFIDPVSTGYSRAVKGGKPGVYHGYQGDLESVAEVIRLWTSRNGRWMSP
                     KFLAGESYGTLRAAALADHLQTRYGMYLNGVILISSVLDLSSIDFAEQRNDRAHAAYL
                     PLYAAVAHYHGKHPGRSLDEVVAEASEYAARDYPWALARGARLRADERAEVAATVARL
                     AGVSADYVDRAELRIEHWRFFGELLRDERLTVGRLDSRFTGPAASSIAEEMDADPSFD
                     AIVGPYAAAMNHYVRAELEFGSDLHYEQISERVNPWSYKDFEGRPIDVSPRLERAMRA
                     NPHLQVHVAYGIYDGATPPAAA"
     gene            complement(1492..2667)
                     /gene="galK"
                     /locus_tag="PLB86_11460"
     CDS             complement(1492..2667)
                     /gene="galK"
                     /locus_tag="PLB86_11460"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRC65801.1"
                     /translation="MAMAPIDRALRLFQQAFGGEPAGVWSAPGRVNLIGEHTDYNGGL
                     ALPIALPQRTYAAVRVRPDGVLRVVSDGMPGGPVSVPLTEVGPGSPGGWAAYVAGVFW
                     ALRDAGDAVPGADVALSSSVPVGAGLSSSAAVECSVGAALSDLTDLGLLADLDGRRRL
                     ATVCQRAENDIAGAPTGGMDQLASMLGTAGHALLLDCRDGSTQAVPFDLAAHGLALLV
                     VDTRAPHALVDGQYAARRHTCQEAATALGVSSLREVGLPYLAHTLARLPDDVTRRRVR
                     HVVTEIDRVGRFADAVEAADWRLAGTLMVASHESLRDDYEVSCQELDLVVETAMAAGA
                     WGARMTGGGFGGCAIALVDAASASSVEAAVLAAFAGAGWSSPVPFLVTPADGAGRDQ"
     gene            2723..3328
                     /locus_tag="PLB86_11465"
     CDS             2723..3328
                     /locus_tag="PLB86_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HRC65802.1"
                     /translation="MSADLRSRDAVTMGPGADWSADAQDHRQTIGVAIRVPAPFGPLL
                     QAKRAEFKDPLADLIPAHVTLLGPTVIHQVARPALVEHLEQVSREVGPFPMALRGTGT
                     FRPVSDVVFVQVARGISGCERLEQRIRSGEYAAELTFPYHPHVTVAHDVAEADLDRAF
                     DDLADFRADFDVEEIWLFEQDAAGQWRAVRSFRLTGEEQTR"
     gene            3325..4515
                     /locus_tag="PLB86_11470"
     CDS             3325..4515
                     /locus_tag="PLB86_11470"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRC65803.1"
                     /translation="MTAAEASRAEPEAGRAEAGRAELSAIRRLWARAQRTEAWRAWQR
                     YGVAQGGLLAAGIGYFSFFSLFPAVALAAVVFGFILQGRPDLLAAIGDALNAALPGFV
                     KTDANPSGLVALTAPETATLSWTGAVAIVTLVAGGLGWIGSLGEGLRAMFGVRKHPGN
                     IVVAKARDLVVLGLLGVALLVSATLTSAVGAAGAWSAQHLGLGEHPWLVGIAGVIVSL
                     LVDMAIMVVLLRVLTGLKLPWPVVRAGALIGGGAMTLLKLVGAQLVTRATSNPVFGSL
                     VVVVGLLFWLNLMAKVVLLSAAWAAGDLDDSGMPSTRAAAAASTQAAGRDMAGPDDAD
                     GALDGASGGIDAGDSRARAVNGIPNLGVRSKDRVTLAAGAVLGASAAIAGGAVRRLAR
                     PRQR"
BASE COUNT          655 a         1622 c         1593 g          659 t
ORIGIN      
        1 tcagccgccg ccgggggagt ggccccgtca tagatcccat aggccacatg cacctgcagg
       61 tgcgggttgg cccgcatcgc ccgctccaac cgtggcgaga catcgatcgg ccgaccctcg
      121 aaatccttgt aggaccacgg gttcacccgc tcggagatct gctcgtagtg caggtcgctg
      181 ccgaactcca gctcagcgcg cacgtagtgg ttcatcgccg cggcatacgg cccgacgatc
      241 gcgtcgaagg acgggtccgc gtccatctcc tcggcaatgc tgctggccgc cggcccggtg
      301 aaccgcgagt ccaaccgccc gaccgtcagc cgctcgtccc gcaacaactc cccgaagaac
      361 cgccagtgct cgatccgcag ctcggcgcga tcgacgtagt cggccgacac ccccgcgagc
      421 cgcgctaccg tcgccgcgac ctccgcacgt tcgtcggcgc gcagccgagc cccacgcgcc
      481 agcgcccacg ggtagtcgcg ggcggcatac tcgctcgcct cggcgaccac ctcgtcgagg
      541 gaacggccgg gatgcttgcc gtgatagtgg gcgacggcgg catacaaggg gaggtatgcc
      601 gcgtgcgccc ggtcgttgcg ctgctccgcg aagtcgatcg acgacaggtc caagaccgac
      661 gagatgagga tgacgccgtt gaggtacatg ccgtagcggg tctgcaggtg gtcggcgagt
      721 gccgccgcgc gcagggtgcc gtaggactcg ccggcgagga acttgggcga catccagcgg
      781 ccgttgcgcg aggtccacag gcggatgacc tcggcgacgc tctcaaggtc gccctgatag
      841 ccgtggtaga caccgggttt cccgcccttg accgcccgcg agtagccggt cgacaccggg
      901 tcgatgaaca ccaggtcgct ctcggcgagc agtgtctcgg cgttgtcggc cagggcatac
      961 ggcggcggca gcagggcacc gacatcgccc atgaccaccc gccgcggccc gagcaggccc
     1021 aggtgcagcc acaccgacga cgaccctggc ccgccgttga acgcgaacgt cacgggccgc
     1081 gccgcccgct cccgcccgga gagaccttcg cggacgtagg acgtgatcga cacctgcgcc
     1141 ttggccgggt ggccgccgaa cacgccgtcc tcgtagacct cctcgcgcaa caccagtcgc
     1201 ccggtcgtcg cgctgtagtc caggacctgc ccatcggaga gcgtcacggt atgccgtgtc
     1261 gtcaccaact cgtcggtcgg ctcgggctcg gcagccttgc cgtccggtgc agccccggct
     1321 gggttggcgc tcgtggccga gggagtgtgg gtgggtgccg aagagagggg ctgcggcgcc
     1381 gcggactcgg gcgtcgcgga cgaggtcgcg tcggtcatca cctcacccta gccgtggccg
     1441 cttcacgtcg gcccgtcacg ctgggttgcc ggtcgcgccc gtcccgccgc attactggtc
     1501 gcgccctgct ccatcggccg gcgtgacaag gaagggaacc gggctagacc acccggcccc
     1561 ggcgaacgcc gccagcaccg ccgcctcgac ggagctcgcg gaggcggcat cgaccagcgc
     1621 gatcgcgcac ccgccgaacc cgccgcctgt catccgtgcc ccccacgccc cggcagccat
     1681 cgccgtctcg acgacgagat cgagctcctg acaactcacc tcgtagtcgt cgcggaggga
     1741 ctcgtgcgag gccaccatga gcgtccccgc gagccgccag tcagcggctt ccacggcgtc
     1801 ggcgaaccgg ccgacgcggt cgatctcggt gacgacgtga cggactcgac ggcgggtcac
     1861 gtcgtcgggg agccgtgcga gcgtgtgggc gaggtaagga agcccgacct cacgcaggct
     1921 gctcaccccg agcgccgtgg cggcttcctg gcaggtatgc cgtcgcgcgg catactgccc
     1981 gtccacgagg gcgtgcgggg cgcgcgtgtc gacgacgagc aacgccaacc cgtgagcggc
     2041 caggtcgaaa ggaaccgcct gggtcgaccc gtcacggcag tccaacagca gggcgtgtcc
     2101 ggccgtgccg agcatcgacg ccagttggtc catgccgccg gtgggtgccc cggcgatgtc
     2161 gttctcggcc cgctggcaga ccgtggccag ccgacgacgt ccgtcgaggt cggcgagcag
     2221 gccgaggtcg gtgaggtcgg acagcgcggc gccgaccgaa cactccacgg ccgccgacga
     2281 ggacaggccg gctcccacgg gcaccgaact ggacagagcc acatcggcgc cgggcacggc
     2341 atcccccgcg tcacgaaggg cccagaagac tccggcgacg taggccgccc acccgccggg
     2401 ggagccgggg cccacctcgg tgagcggcac gctgacgggg ccacccggca taccgtcgct
     2461 gacgacccgc aggacgccgt cggggcgcac gcggacggcc gcgtaggtgc gttgcgggag
     2521 cgcgatgggc agcgccaggc cgccgttgta gtcggtgtgc tcaccgatga ggttgacccg
     2581 accgggtgcc gaccagaccc cggcgggctc gccgccgaac gcttgctgga agagtcgcag
     2641 cgctcgatcg atcggtgcca tcgccatgtc gggaagcgta gcgatgacga acgggcccaa
     2701 cgaagtcgat acgctgacac ccatgtccgc cgacctgagg tctcgagacg ccgtcacgat
     2761 ggggcctggt gcggactggt ccgcggacgc gcaggatcac cgccagacca tcggcgtggc
     2821 catccgcgtc cccgctccgt tcggtccgtt gttgcaggcc aaacgggccg agttcaagga
     2881 cccgctcgcc gatctcatcc cggcccacgt cacactcctg ggcccgaccg tcatccacca
     2941 ggtggcgcgt ccggctctgg tcgagcacct ggagcaggtg agccgcgagg tgggcccgtt
     3001 cccgatggcg ttacgcggga cgggcacgtt ccggccggtc tcggatgtcg tgttcgtcca
     3061 ggtggcccga ggcatctccg gatgcgagcg gcttgagcag cgcatccgca gcggggagta
     3121 tgccgcggag ctcaccttcc cctaccaccc gcacgtcacg gtcgcccacg acgttgccga
     3181 agccgacctc gaccgggcct tcgacgacct cgccgatttc cgtgccgact tcgacgtcga
     3241 ggagatctgg ctcttcgagc aggatgctgc gggacagtgg cgggcggtgc gatcgttccg
     3301 gctcaccggt gaggagcaga cccgatgacc gcggctgaag ccagtcgtgc ggagcctgaa
     3361 gccggtcgcg cggaagcagg tcgcgccgag ctcagcgcga tccgccggct atgggcccgg
     3421 gcgcagcgca ccgaagcctg gcgggcctgg cagcgctatg gggtggccca ggggggcctg
     3481 ttggccgccg ggatcggcta cttctcgttc ttctcgctct ttcccgctgt ggcactggca
     3541 gcagtggtct tcggcttcat cctgcagggc cggcctgacc tgctcgcggc catcggcgat
     3601 gccctcaacg ccgcgctccc gggcttcgtc aagaccgacg ccaacccgag cgggctggtg
     3661 gcgttgaccg ctcccgaaac cgccaccctc agctggacgg gagcggtcgc gatcgtcacc
     3721 ctcgtggccg gtggtctcgg ctggatcggc tcgctaggcg aagggttgcg ggcgatgttc
     3781 ggggtgcgca aacatcccgg caacatcgtc gtcgccaaag cgcgcgacct cgtcgtcctg
     3841 ggcctgttgg gggtggccct tctcgtgtcg gccaccctca cctcggccgt cggtgcggcc
     3901 ggagcgtgga gcgcccagca cctcgggctg ggtgagcatc cgtggctggt cggcatcgcc
     3961 ggggtcatcg tctcgctgct cgtcgacatg gcgatcatgg tcgtcctgct gcgcgtgctc
     4021 accggtctga aactgccctg gccggtcgtc cgggccggcg ccctgatcgg cggcggcgcc
     4081 atgacgctgc tcaaactcgt tggggcacaa ctcgtgacgc gggccacgag caacccggtc
     4141 ttcgggtcgc tcgtcgttgt cgttgggctg ttgttctggc tcaacctgat ggccaaggtc
     4201 gtgctcctct cggcagcgtg ggcggccggt gacctcgacg actcaggtat gccgtccacc
     4261 cgcgctgccg cagccgcctc gacccaggcg gccggccgag acatggcggg gcctgatgac
     4321 gccgacgggg cgctcgatgg ggccagcggc gggatcgatg ccggcgactc gcgggcccga
     4381 gcggtgaacg gcatacccaa cctgggagtc cggagcaagg accgggtcac gctcgctgcg
     4441 ggcgccgtgc tgggcgccag cgccgcgatc gcgggcggcg cagttcgccg cctggcgcgc
     4501 cctcgtcagc gctgatagac ccctcgggc
//
LOCUS       DAORPW010000189         4509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_5773_length_4509_cov_900.368208, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000189 DAORPW010000000
VERSION     DAORPW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5773_length_4509_cov_900.368208"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1389
                     /gene="glyA"
                     /locus_tag="PLB86_11475"
     CDS             115..1389
                     /gene="glyA"
                     /locus_tag="PLB86_11475"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRC65804.1"
                     /translation="MSEFYGSDFDALTAFDPDIAGVLLSELARIRGGLQLIASENISS
                     PAVLTALGSTLSNKYAEGYPGRRYYGGCAEVDKAEQIAIDRCAALFGADHANVQPHSG
                     ASANQAVYGAFLKPGETILAMSLPHGGHLTHGSKVSFSGKWFNAVHYGVDKDTEDIDY
                     DQVEALAKEHRPKVILAGGSAIPRLIDFARFRAIADEIGAIFWVDAAHFIGLVAGKAI
                     PSPVPYADVVSFTTHKVLRGPRSGAIVCTAQHAAAIDKAVFPMMQGGPQMHTIAAKAV
                     NFKECGTPEYAEYAARVVRNAAVLATELGEHGIRPTTGGTDTHLSLHDLQGVGVTGKD
                     AEARADAAGIVLNKNAIPFDPAPPSVASGIRVGTPCVTTQGMGEEEMRRIARLIATAV
                     TKGDADPEHAVSKAVRAEVTELVTAFPAYPRP"
     gene            1441..2541
                     /locus_tag="PLB86_11480"
     CDS             1441..2541
                     /locus_tag="PLB86_11480"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRC65805.1"
                     /translation="MREYLLICVVALAVTYLMTPFVRALAVVIKAVTPLRERDVHTVP
                     IPRLGGVAIFAGLAAATVVARQLPYLKGVYVSGQMTGVLLGAAVICLIGAIDDVRELD
                     WMTKFAGQVLAAGVMAYKGVIMLHVPFFGTSTVLPQPVLIGVTVLIVLVTTNAVNFID
                     GLDGLAAGIVAIAALAFFIYSYLLSYTFNPPNVFSTATFVTAATLGCCLGFLPHNFNP
                     ARLFMGDSGALLLGLLLAAATISITGNVDPSAVSGDQVTAALLPIIAPLAVLSIPFLD
                     MVLAVFRRARAGQKPWHPDSKHLHHRMLQIGHGHRGAVLILYLWAAVLAFGTVSWAIV
                     GGWPTLAGIAVAVVVAVTLTAYLPRWMRNRRL"
     gene            2594..3061
                     /locus_tag="PLB86_11485"
     CDS             2594..3061
                     /locus_tag="PLB86_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65806.1"
                     /translation="MTPTPGPLDGATAGPADEARESDAFAELLRGAFRPTALVGVLAV
                     VGSVFSGVTAAWSAALGAAIVLGFFTASLLVMKRTAHLPPTTVMAVVMITYTVKVIVL
                     GIVLFAVRDASWLNGYAAGVTITVCALVWLFFEMRAYKRLRIFAFSPDEKAGA"
     gene            3519..4310
                     /gene="atpB"
                     /locus_tag="PLB86_11490"
     CDS             3519..4310
                     /gene="atpB"
                     /locus_tag="PLB86_11490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRC65807.1"
                     /translation="MSIIALGMEGGGDGFTAPDASIFWEPLIGEGATAITRPVFLMVI
                     AGALVAWFLLATTKRRALVPSKGQMMTEGAYGLVRNSIAKDIIGSHEFLRFVPLLFTM
                     FLLILVNNFFGVIPPFNYPTMSRIGFPVALALVIWVVFHVVGMKKHGFVGYWKSLVPS
                     GLPGGMVPAIFILELITDLFTRPVTLGLRLFGNMFAGHILMALFISGGWYMLQAGGIL
                     PVAGAVTWAFAIVMTLFEMLVEFLQAYVFTLLSALYIAGAVADSH"
BASE COUNT          661 a         1612 c         1422 g          814 t
ORIGIN      
        1 gcggggcggg gcgccgcgcc acgctccgtg agcggacggg gcgggaggtg cggcacacgg
       61 catacagtgg cttcttgccg gtcccccctt ccccgagagg cgcagcgaga ccccatgagc
      121 gagttctacg gcagcgattt cgacgcactg acggccttcg acccggacat cgcgggcgtc
      181 ctgctgtccg agctcgcccg gatccgcggc ggcctgcaac tcatcgccag cgagaacatc
      241 tccagccccg cggtgctcac cgccctgggc tcgaccctct ccaacaagta tgccgagggc
      301 taccccggcc gtcgctacta cggcggctgc gccgaggtcg acaaggcgga gcagatcgcc
      361 atcgaccgtt gtgccgcgct cttcggcgcc gaccacgcca acgtccagcc gcacagcggg
      421 gccagcgcca accaggccgt ctacggcgcg ttcctcaagc ccggcgagac catcctggcg
      481 atgagcctgc cgcacggcgg ccacctcacc cacggcagca aggtctcctt cagcggcaag
      541 tggttcaacg cggtgcacta cggcgtcgac aaggacaccg aggacatcga ctacgaccag
      601 gtcgaggccc tcgccaagga gcaccggccc aaggtcatcc tcgccggcgg ctccgcgatc
      661 ccgcggctca tcgacttcgc gcggttccgc gccatcgccg acgagatcgg tgcgatcttc
      721 tgggtcgacg cggcgcactt catcggcctg gtcgcaggca aggcgatccc cagcccggtg
      781 ccctacgccg acgtcgtgtc cttcacgacc cacaaggtgc tgcgcggccc gcgctcgggc
      841 gcgatcgtct gcaccgcgca gcacgccgcc gctatcgaca aggccgtctt cccgatgatg
      901 cagggcgggc cgcagatgca cacgatcgcg gccaaggcgg tcaacttcaa ggagtgcggg
      961 accccggaat acgcggagta tgccgcgcgg gtcgttcgca acgcggccgt cctcgcgacc
     1021 gagttgggtg agcacgggat ccgtccgacc accggtggca ccgacaccca cttgtcgctg
     1081 cacgacctgc agggtgtggg cgtgacgggc aaggacgccg aggcccgggc cgacgcggcg
     1141 ggcatcgtcc tcaacaagaa cgccatcccc ttcgaccccg cgccgccgag cgttgcctcg
     1201 ggcatccggg tcggcactcc gtgtgtgacc acccaaggca tgggggagga ggagatgcgt
     1261 cggatcgccc ggctcatcgc caccgccgtc acgaagggcg acgccgaccc cgagcacgcc
     1321 gtctccaagg cggtgcgcgc ggaggtgacc gagttggtca ctgccttccc ggcataccct
     1381 cgcccctagt cctccaccgc cctgctgggc gatcccgacc agacgaagcg gccaccctct
     1441 gtgcgcgagt acctgctcat ctgtgtggtg gcgctggccg tcacctacct gatgacgccg
     1501 ttcgtccgcg ccctggcagt ggtcatcaag gcggtcaccc cgctgcggga acgcgacgtg
     1561 cacaccgtcc cgatcccgcg gctgggcggg gtggcgatct ttgcggggct cgctgcggcg
     1621 accgtcgtcg cacgccaact gccttatctc aagggcgttt acgtcagtgg gcagatgacc
     1681 ggcgtgctcc tgggcgctgc ggtgatctgc ctcatcggcg ccatcgacga cgtccgtgag
     1741 ttggactgga tgacgaagtt cgccggtcag gtgctggccg cgggggtcat ggcctacaag
     1801 ggcgtgatca tgctgcacgt gccgttcttc ggcacgagca ccgttctgcc gcaaccggtt
     1861 ctcatcggtg tcaccgtgct catcgtgctc gtgacgacca atgcggtgaa cttcatcgac
     1921 ggccttgacg ggctggcggc gggcatcgtc gccatcgcgg ccctggcgtt cttcatctac
     1981 tcctacttgt tgagctacac cttcaaccca ccgaacgtct tctccactgc gacgttcgtc
     2041 accgcggcga ccctgggctg ttgcctgggc ttcctgccgc acaacttcaa ccccgcccgg
     2101 ctcttcatgg gcgattcggg cgcgctgctg ctcggcctgc tcctcgccgc cgcaacgatc
     2161 tcgatcaccg gcaacgtcga tcccagcgcc gtcagcggcg atcaggtgac ggcggccctg
     2221 ctgccgatca tcgccccgct ggccgtgttg tcgattccgt tcctcgacat ggtgctggcc
     2281 gtgttccggc gcgcccgggc cggccagaag ccgtggcacc ccgacagcaa gcatctgcac
     2341 caccgcatgc tgcagatcgg ccacgggcac cgaggagcgg tcctcatcct ctatctgtgg
     2401 gccgcggtcc tcgcgttcgg gacggtgtcg tgggcgatcg tcggcggttg gcctaccctg
     2461 gccggcatcg ccgtggcggt cgtcgtggcg gtgacgctca cggcatacct gccgcgttgg
     2521 atgcgcaacc gacgcttgtg atacctttca caagctcccc tcgcaggccc cacgaccacc
     2581 cccaaggcgg acgatgaccc ccacccccgg accgttggac ggcgccaccg ctggcccggc
     2641 cgatgaggcg cgcgaaagcg acgccttcgc cgagttgctg cgcggggcct tccgtcccac
     2701 ggccctcgtc ggagtcctgg cggtcgtcgg atcggtgttc tcgggcgtca ccgcagcgtg
     2761 gtcggccgcc ctcggcgcgg ccatcgtcct gggcttcttc accgccagcc tgctggtcat
     2821 gaaacgcacc gcgcacctgc cgccgacgac cgtgatggct gtggtgatga tcacctacac
     2881 cgtcaaggtg atcgtcctcg gaatcgtcct cttcgcggtc cgcgacgcct cctggctcaa
     2941 cgggtatgcc gcgggcgtca ccatcaccgt gtgcgcgctg gtgtggttgt tcttcgagat
     3001 gcgcgcctac aagcggctgc gcatcttcgc gttctcgccg gatgagaagg cgggggcgtg
     3061 atcctgctca ccgaccccct ccggtatgcc gcgcacaccc gtgcgcgggc ccggcacgac
     3121 ggccgtctgc gagaggtttc cagccatgtc tccgacccct ccatcgcccc cttccggcgg
     3181 gacccccgag gagcgagaag cgttgcgcga gttcaatgtg cgcgaagcgg actcggcatg
     3241 gaaggctgtg gcgtacttga ttacgggacc tgcgatctac ggggggctgg ggtggcttct
     3301 cgatcgttgg tggggcacgt ccttcatgac tccggtaggc atcgtcgggg gcatggcgct
     3361 gtcgatctac ctcatctggt tccggtacgg ttctcactga aaccggttct gacctatccc
     3421 tgcttcgcgg cgccctcggc ggcttcgcaa cagccctcgg aacgaacggg tttcagccga
     3481 agcggccacc cggtcctgac cactcgaagg agagtctcgt gagcatcatc gctctcggta
     3541 tggagggtgg gggcgacggc ttcactgctc ccgacgcctc gatcttctgg gagccgctca
     3601 tcggtgaagg agccaccgcg atcacgcggc cggtcttcct catggtgatc gctggagccc
     3661 tcgtggcctg gttcctgctg gccacgacca agcgccgcgc gctcgtgccg agcaagggtc
     3721 agatgatgac cgagggcgcc tacggcctgg tgcgcaacag catcgccaag gacatcatcg
     3781 gcagccacga gttcctgcgc ttcgtgccgc tgctgttcac gatgttcctg ctgatcctcg
     3841 tcaacaactt cttcggcgtc atcccgccgt tcaactaccc gacgatgagc cgcatcggtt
     3901 tcccggtcgc gctggctctc gtcatctggg tcgtcttcca cgtcgtgggc atgaagaagc
     3961 acggcttcgt cggctactgg aagtcgctgg tccccagcgg tcttccgggc ggcatggttc
     4021 cggcgatctt catccttgag ctcatcaccg acctgttcac ccggccggtc acgctcggct
     4081 tgcgactgtt cggcaacatg ttcgccgggc acatccttat ggcgctgttc atctccggcg
     4141 gttggtacat gctgcaggcc ggagggatcc tccccgtcgc cggcgccgtc acctgggcgt
     4201 tcgccattgt catgaccctg ttcgagatgc tggtcgagtt ccttcaggcc tacgtcttca
     4261 cgctgctgtc cgcgctctac atcgccggcg ccgtcgccga ctcgcactga cctgctcgac
     4321 ctgcttgacc cgcactgacg ttgtttgaac cgcactgacc tgcacgacct cagaccaccc
     4381 gattgacccc accgggctca cccggttgga ccaaacgaaa aggaaatgga atcgacatga
     4441 ccggcaacat tgcgatcctc ggttacggcc tctccgccat cggccccggc atcggtgtcg
     4501 gcctgatct
//
LOCUS       DAORPW010000190         4413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6004_length_4413_cov_1002.390776, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000190 DAORPW010000000
VERSION     DAORPW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6004_length_4413_cov_1002.390776"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..972)
                     /locus_tag="PLB86_11495"
     CDS             complement(16..972)
                     /locus_tag="PLB86_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRC65808.1"
                     /translation="MTVVALPAGLVAAAAGRPADVLLAGPSARHAIDGDSWLRGLPRT
                     VADVCADWELTPDGPARHGACAIALPVVSADGPAMLKVTWPHHEAAGEHLALRHWGGQ
                     AAVRLLRADPSRWALLLERLDADRDLHGIPMDEACEVVGDLLRHLKVPAPPTVPTLRE
                     YAARKADAYADVLAGGAGPVPRRFVDQARALAADFATDDSGGGWLLHTDLHCSNVLRG
                     IRDGRPAWVAIDPKPAAGDRAFDVVALLWDRPDELGTGADLRWGLRRRVEIVCEAAEI
                     DEDRARSWAIVREMDNALEVAHDPAAADRVSLAVAIIKAMQP"
     gene            complement(969..2009)
                     /locus_tag="PLB86_11500"
     CDS             complement(969..2009)
                     /locus_tag="PLB86_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65809.1"
                     /translation="MSRAPLTAGLGGIPRRMPPRRTVLRAAGLAGLSAVAGIGLAGCG
                     IRLEDDAPTIPFVSRQPIPDEALLVAAYRRATALAGVAGRATGVPLAPEVTQRHTRQA
                     QVLRAILETGQVPERIISGPPGSTATATSGPTAPVDGTATTASTSAPTNAVPPPVTAG
                     ELARAARDTADASIAAAGSFVTQRALGVAIAAHDAAGAASLAAPPVWPTRDPLPAPVA
                     TPLVGVTRACEYALEVAAAHLLDTDRESLLATVAALTRREATLAAALTPDPLPALGYH
                     LPFPVTGPDDARRLVSVVLRNLVDRGLDDLDRIPQGSSGLLEVVRLEAEAVTLAVAHG
                     VAWPTMPGLALG"
     gene            2152..2802
                     /locus_tag="PLB86_11505"
     CDS             2152..2802
                     /locus_tag="PLB86_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRC65810.1"
                     /translation="MQASLVRSIVEAPLAAVDLVVEDVTVTAAGRRSVVRIAVDRSLA
                     GLANTDTTSFVAPLSLDEVADATRVVDAALDSDTRVGNAPYVLEVSSPGVDRPLTQPR
                     HLRRNVGRLVRLRLKEGAERSGRIASVSDLVIVLTPDPAADPAATAAGPVEIPVDQVR
                     DGRVIIEFGRIDEVDLDDTDAEIDETDETDETDDTGELDETDETDDELDEPSSEEG"
     gene            2802..3992
                     /gene="nusA"
                     /locus_tag="PLB86_11510"
     CDS             2802..3992
                     /gene="nusA"
                     /locus_tag="PLB86_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRC65811.1"
                     /translation="MDIDLAALRALERERDLSLEVLIPAIEQALLTAYHRTDGSFKIA
                     RVELDRKTGHVVVLAREEGPLLPPTEEGERPTRGEPGPEFDDTPDGFGRVAAATARQV
                     IVQRLRDLEDEAILGDFKGREGDIVSGIVQQGSDPRNVLVDFGTVEGMLPQAEQVPGE
                     VYRHGERLRCYVVSVKRGPKGPQIALSRTHPNLVRSLFKLEVPEIADGTVEIAAMIRE
                     AGHRTKIAVRSRVPGVNAKGACIGPMGARVRAVMNELGGEKIDIVDYSDDPREFIAAA
                     LSPARVQSVEVVDPVLRAARAIVPDYQLSLAIGKEGQNARLAAKLTGWRIDIRSDTAV
                     AENPPTSDAPPASATRPAGDRRPQQGGGGRHTGSRGTGRGGSGAGPSGPPRRGGPTGH
                     PATG"
     gene            3997..4395
                     /locus_tag="PLB86_11515"
     CDS             3997..4395
                     /locus_tag="PLB86_11515"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRC65812.1"
                     /translation="MGTDRTGLRSASFPYAAQQGSPVRTCVGCRRPDSWLVLVRVVAF
                     VEAGHARLVPDVRHRLPGRGAWLHPETACLEVAVRKRAFGRALRLQVPADAAALTAYL
                     RDLVPPLEPTMSEQPQGGLDADEHAMSTQQ"
BASE COUNT          687 a         1573 c         1572 g          581 t
ORIGIN      
        1 cgcccatcgc cccgctcatg gctgcatcgc cttgatgatc gccaccgcaa ggctgacgcg
       61 gtccgcagcc gcggggtcgt gagccacctc aagtgcgttg tccatctcgc ggacgatagc
      121 ccaggaccga gcccggtcct cgtcgatttc cgcggcctcg cacacgatct ccacccgtcg
      181 acgcagcccc caccgaaggt cggccccggt gcccaactcg tcgggccggt cccacaacag
      241 cgcgaccacg tcgaaggcgc ggtcgccggc cgccggcttg gggtcgatcg ccacccatgc
      301 cggccggccg tcgcggatcc cgcgcaggac gttggagcag tgcagatcgg tgtgcagcag
      361 ccatccaccg cctgagtcat cagtggcgaa atctgcggcc aacgccctcg cctggtcgac
      421 gaaccgccga ggcaccgggc cggcgccacc ggcgagcaca tccgcgtacg catccgcctt
      481 ccgcgcggca tactcacgca gcgttggcac ggtcggggga gcgggcacct tcaggtggcg
      541 cagcaggtca ccgacgacct cgcacgcctc atccatgggt atgccgtgca ggtcgcggtc
      601 ggcgtcgagg cgctcgagca gcagggccca ccgggacggg tcggctcgca ggagccgaac
      661 cgccgcttgg ccaccccagt gccgcaacgc gagatgctcg cccgcggcct cgtgatgcgg
      721 ccaggtgacc ttgagcatgg ccgggccgtc ggcgctgaca accgggaggg cgatcgcgca
      781 ggccccgtgc cgggccgggc cgtcgggcgt caactcccaa tcggcgcaca cgtcggcaac
      841 cgtccgcggc agcccgcgca accacgagtc gccgtcgatc gcgtgtcgtg cggacgggcc
      901 ggccagcagg acatccgccg gacgccccgc cgccgcggcg acgagaccgg cgggaagcgc
      961 gaccaccgtc accccagcgc cagcccgggc atcgtcggcc aggcgacgcc gtgggcgacg
     1021 gccagcgtca ccgcttcagc ctcaagccgg accacttcga gcaggccaga ggacccctgc
     1081 gggatccgat cgagatcgtc caacccgcgg tccacgaggt tgcgaaggac gacgctcacc
     1141 agccgccgag cgtcgtccgg cccggtgacg gggaagggca ggtgataacc caaagcagga
     1201 agcgggtccg gggtcagcgc ggcggccagc gtcgcctcgc ggcgggtcag cgcggcgacc
     1261 gtggcgagga gcgactcacg gtccgtgtcg agcagatgcg ctgcagcgac ctcgagggcg
     1321 tattcgcagg ctcgcgtcac ccccaccaac ggcgtggcga cgggggcggg cagcgggtcg
     1381 cgggtcggcc agacgggcgg cgccgcgagg cttgccgcgc ccgcagcgtc atgggcggcg
     1441 atggcgacac ccagggctcg ctgggtgacg aacgacccgg cagccgcgat cgaggcatcg
     1501 gcggtgtcgc gagccgcgcg ggccaactcc cccgcggtga ccggcggcgg caccgcgttc
     1561 gtgggtgccg atgtggacgc cgtggtcgcg gttccatcga cgggcgcggt cggccccgag
     1621 gtcgccgtcg cggtcgagcc gggcggcccg ctgatgattc gttcggggac ctgccccgtt
     1681 tcgaggatcg cacgcagcac ctgggcctga cgggtatgcc gttgcgtcac ctcaggcgcg
     1741 agaggcaccc ccgtagcccg gccggcgact ccagccaacg ccgtcgcccg tcgataggcc
     1801 gcgacgagca acgcctcgtc ggggatcggt tggcggctga caaacgggat ggtgggagcg
     1861 tcgtcctcca accggatccc acacccggcc aggccgatgc ccgccacggc cgacaggccg
     1921 gccaaccccg ccgcgcgcaa cacggtgcgg cggggcggca tacgacgggg tatgccgccc
     1981 aaaccggcgg tgaggggggc ccgagacacg gcccgatcat ggcatgggac ggtgcccgcg
     2041 ccgctcgact gacgtcaggg cagcggtagc gccgttacag tggtgtcacc cttcggctgg
     2101 ccgacgggtc ccgcgagtgc ggaaaatcga caagagcagg aggtgtgcgc cgtgcaggca
     2161 agccttgtcc gctccattgt cgaagcgccg ctggccgcgg tggatctggt cgtggaggac
     2221 gtcaccgtga ccgctgccgg gcgtcgctcg gtggttcgga tcgcggtcga ccgctccctg
     2281 gccggcctcg ccaacactga cacgacctcg ttcgtggcgc ctctgtcctt ggacgaggtc
     2341 gccgatgcca cccgagtcgt cgacgcagcc ctcgactccg acacccgggt gggcaacgcg
     2401 ccctacgtgc tcgaggtgag ctcgcccggc gtggataggc cgctgaccca gcctcgtcac
     2461 ctgcgccgca acgtcgggcg cctcgtgcgg ttgcgcctga aggagggcgc ggagcgctcc
     2521 gggcggatcg cctccgtgag cgatctggtc atcgtcctga caccggaccc ggctgccgat
     2581 ccggcggcca ccgccgcagg gccggtcgag atcccggtgg accaggtgcg cgacggccgc
     2641 gtcatcatcg agttcgggcg gatcgacgag gtcgacttgg acgacacaga cgccgagatc
     2701 gacgagaccg acgagaccga cgagaccgat gacacaggcg agcttgacga gacagacgag
     2761 acagacgacg agttggacga gccgagcagt gaggagggct gatggacatc gacctggcag
     2821 cgctacgggc gttggaacgc gaacgcgacc tcagccttga ggtgttgatc ccggcgatcg
     2881 agcaggccct gctgaccgcc tatcaccgca ccgacgggtc gttcaagatc gctcgcgtgg
     2941 agttggaccg caagaccggc catgtggttg tcctcgcgcg cgaggagggc ccgctgctgc
     3001 ccccgaccga ggagggcgaa cgccccactc gcggcgagcc cggcccggag ttcgacgaca
     3061 cgccggacgg tttcgggcga gtcgctgcgg cgaccgcgcg gcaggtcatc gtccagcgac
     3121 tgcgcgacct ggaggacgag gcgatcctcg gtgacttcaa gggtcgcgag ggcgacatcg
     3181 tctccgggat cgtccagcag gggtcagacc ctcgcaacgt cctggtcgac ttcgggacgg
     3241 tcgaaggcat gctcccgcag gccgagcagg tgcccggcga ggtctaccgg cacggtgagc
     3301 ggctgcgctg ctacgtcgtc agcgtcaagc gtggtcccaa gggcccgcag attgctctgt
     3361 cacgcaccca ccccaacctc gtccggtcgc tgttcaaact cgaggtgccc gagatcgcgg
     3421 acggcaccgt cgagatcgcc gcgatgatcc gggaagccgg ccaccgcacc aagatcgcgg
     3481 tgcgctcccg ggtccccggg gtcaacgcga agggggcatg catcggcccg atgggtgccc
     3541 gggtccgggc cgtcatgaac gagttgggcg gggagaagat cgacatcgtc gactactccg
     3601 acgacccgcg cgagttcatc gcggcggccc tgtcgccggc acgcgtgcag tcggtcgagg
     3661 tcgtcgaccc ggtgctgcgt gccgcccggg caatcgttcc cgactaccaa ctgtcattgg
     3721 cgatcggcaa ggagggccag aacgcccgac tggccgccaa actcaccggt tggcggatcg
     3781 acatccgctc cgacaccgct gtcgccgaga accctccgac gtctgatgcg ccgcctgcca
     3841 gcgccacccg gccggccggt gaccgccgcc cgcagcaggg cggcggcgga cggcataccg
     3901 gctcgcgcgg cactggacgc ggtggctctg gcgccggtcc gtccggcccg ccgcgccggg
     3961 ggggacccac ggggcacccc gccacggggt agactgatgg ggaccgaccg gactggactc
     4021 cgttcggcgt ccttcccgta tgccgcgcaa cagggatccc ctgtgcgcac ctgcgtggga
     4081 tgtcgccgcc cggatagctg gttggttctc gtgcgagtgg tcgccttcgt ggaagccgga
     4141 catgcccggt tggtccccga tgtgcgacat cggctccccg ggcgcggcgc atggctgcac
     4201 ccggagaccg cttgtctaga ggtcgccgtc cggaaacggg cgttcggaag agccctgcgc
     4261 ctgcaggtcc ccgccgacgc cgcagccctg acggcatacc tgcgagatct cgttccaccg
     4321 ctcgagccga cgatgtccga acaaccacaa ggcgggttag acgctgatga gcacgcgatg
     4381 agcactcagc aatgagcaag ccccagcact aac
//
LOCUS       DAORPW010000191         4402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6033_length_4402_cov_564.180354, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000191 DAORPW010000000
VERSION     DAORPW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6033_length_4402_cov_564.180354"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="PLB86_11520"
     CDS             complement(<1..100)
                     /locus_tag="PLB86_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRC65813.1"
                     /translation="MSVQDQTSTATHGVVLTDVAASKVKSLLAQEGR"
     gene            complement(307..1545)
                     /locus_tag="PLB86_11525"
     CDS             complement(307..1545)
                     /locus_tag="PLB86_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRC65814.1"
                     /translation="MSPVRVLIAPEGYAGLLAPAEAAAAIARGWSLGAPHDSIEVLPV
                     SDGGPGFIDTVARALGGEVQVVMVDDPWGRPTPATMLVSTDGATVYLEAAQAGGGQLQ
                     QPGFGAQATQATHSPDGTAGDDPAAASSGGVGDLLEAALRLRPRRIVLGLTGSLVLDA
                     GLGMLGRLIPDQPSTAAGRAALSGGAAHLVLLRADNLGALDTAIGRFGATQLVALGDL
                     HQPLLGLAGVAATHGPALGADPEHAQLIEAALGHAVDLVRRARPERRDLLSGTSVRLD
                     RQPGAGAGAGLGFGLAVLGAALRDGATEGPQVVGLAQAVARADLVVTGSTILGWETLR
                     SSASAAVAHAAQEGGIPAIALCGRVEAGRRETMAAGYSGTYAVASSLATWPAFAADPA
                     RALAVRAESVARTWSPPTPA"
     gene            complement(1580..3166)
                     /locus_tag="PLB86_11530"
     CDS             complement(1580..3166)
                     /locus_tag="PLB86_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HRC65815.1"
                     /translation="MLDLLRLLSQQPILLLAILLGLGSLLGSFRYKGVHLGPAAVLFG
                     AIAISSLGTAYGLKLELPEVVGTLGLVLFTYTVGLLSGPNFFASLRRGWVAMVAVVGV
                     FVVVGFVGVLGGRLLGLSSPVIAGTFAGALTNTPALAAATERAGDTTGPTIGYSIAYL
                     YGVAAMLVAAMLVLRRSSDDRARDPLTNLTIRVDLDAPICAHEIVRDSGGTVVFSRIQ
                     HGEENPIEVIDEHEILQAGDLVTVVGPAGAVSGVAERLGHRSSHALQSQRRDLDFRRI
                     TVSQAALAGRTIAELGLDERFGAVATRVRRGDLDMVASEDFVLQMGDRVRVTAPAGRL
                     KEVSAYLGDSERGLSDINPIGFALGLAVGVALGLVHIPAPGGGFTLGAAAGTLLVGLV
                     FGRLVRVGPLVVSMSTSSATSLSTFGMITFLAYAGSRAGERFVASVTSDLGWKVLVLG
                     ALLTTVAAVAIAFVGRSVLKTNSRQLAGMLAGAQTQPAVLAFANDRTGFDLRVGLGYA
                     MVYPAAMIAKILLAQVIAGL"
     gene            3252..>4402
                     /gene="nadA"
                     /locus_tag="PLB86_11535"
     CDS             3252..>4402
                     /gene="nadA"
                     /locus_tag="PLB86_11535"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRC65816.1"
                     /translation="MSIETVTPKHTPLGLLVLGQGRDLHSERGVECPGDLPAPSDPDL
                     VRRARAAKAALGDKVFVLGHHYQRDEVIEFADVTGDSFKLAQQAAARPEAEWIVFCGV
                     HFMAESADILTTDAQTVVLPDLAAGCSMADMATAAQVDDCWDDLIDAGVAEHTIPVSY
                     MNSSAAIKAFTGRHGGTICTSSNARTALEWAFEQTGGVEGTGKVLFLPDQHLGRNTAV
                     RELGLSLDDCVVYDPHRPGGGLTKEAMQAARVILWRGHCSVHGRFTVDAVQAARREIP
                     GVRVIVHPECQYEVCEAADVIGSTEQIIKAVAAAPAGTSWVVGTELNLVKRLSTMFPE
                     QSIAFLEKNVCYCSTMNRIDLPHLVWTLESLAEGRIVNPIRVDPEIAHWA"
BASE COUNT          712 a         1547 c         1556 g          587 t
ORIGIN      
        1 cgcggccctc ctgggccaac aggctcttga ccttcgaggc cgcgacgtcg gtgaggacga
       61 cgccgtgggt cgcggtgctg gtctggtcct ggacgctcat ggtgaatttc ctcggtggtc
      121 gggtgcgtgg gaagtcgtgt cagacggtgc tggaagggtc aaccgtggcc gattgacggt
      181 tgttcccccg cctgcggcat cggtccaatg tacgacgttc gcggctgatt ccctagtccg
      241 tacgtcccgg ttgtgcgcgt ctgtgacgca ccggcgcccg cgtgtggcag cgagtcaggt
      301 gccgcgtcag gcgggggtcg gcggcgacca ggtccgggca acactctcgg cccggacggc
      361 cagggcgcgg gccgggtcgg cggcgaaggc gggccaggtg gccagggagc tcgcgacggc
      421 atacgtcccg gaatagcccg cggccatggt ctcgcgacgg cccgcctcca cgcgcccaca
      481 cagggcgatg gccggtatgc cgccctcctg ggccgcgtgt gcgaccgccg cgctcgcact
      541 ggagcgcagg gtctcccaac ccaggatcgt cgagccggtg acgacgaggt cggcccgagc
      601 gacggcttgg gccaggccga cgacctgcgg tccctcggtc gcgccgtcac gcagcgcggc
      661 tccgagcacg gccagaccga aaccgagccc tgctccggct ccggcgcccg gctgacgatc
      721 cagccgcacg gaggtgccgg acagcaggtc ccgccgctcg ggacgggctc ggcggaccag
      781 atcgacagcg tggcccaggg ccgcctcgat gagttgggcg tgctcggggt cggcgccgag
      841 cgcagggccg tgggtcgccg cgacaccggc gaggccgagc agcggctggt gcaggtcgcc
      901 gagggcgacg agctgggtgg cgccgaatcg cccgatcgcc gtgtcgaggg ctccgaggtt
      961 gtctgcgcgc aggagcacca ggtgggcagc accaccgctc agcgcagccc ggcccgccgc
     1021 agtggacggc tggtcgggga tgagccggcc cagcatcccg agaccggcgt cgagcaccag
     1081 cgatccggtg aggcccagga cgatccgtcg tgggcgcagg cgcagagccg cctccagcag
     1141 gtcacccacg ccgcccgagc tggcggcggc ggggtcatcg ccggccgtgc catcggggct
     1201 gtgggtggcc tgggtggcct gggcgccgaa accgggctgc tgcaactgcc ctccgccggc
     1261 ttgggcggct tcgagataga ccgtggcgcc gtccgtgctg acgagcatcg tcgccggggt
     1321 cgggcgaccc caggggtcat cgaccatcac tacctgcacc tcgccgccca aggccctggc
     1381 cacggtgtcg atgaagcccg gcccgccgtc cgacaccggc agcacctcga tgctgtcgtg
     1441 cggtgccccc aaggaccagc cgcgcgcgat cgccgcagcc gcctcggcag gggcgagcag
     1501 gccggcatac ccctccgggg cgatgagcac acgcacgggc gacatcacac cacgagtcgg
     1561 gccgtctgcg ggaggtgggc tacaacccgg cgatcacttg tgccaggagg atcttggcga
     1621 tcatcgccgc gggatagacc atcgcgtagc ccagcccgac ccggaggtcg aagccggtcc
     1681 ggtcgttggc gaaggcgagc accgccggct gggtctgcgc gccggcgagc atccccgcca
     1741 actgccgcga gttggtcttg agcaccgacc ggccgacgaa cgcgatcgcc acggcggcca
     1801 cggtggtgag gagcgcccca aggacgagca ccttccaccc caggtccgag gtgaccgacg
     1861 cgacgaaccg ctcaccggct cgcgaaccgg catacgcgag gaaggtgatc atgccgaagg
     1921 tcgacaacga ggtcgccgag gacgtggaca tcgagacgac caggggtccc acccgcacca
     1981 ggcgcccgaa gaccaacccg acgagcaggg tcccagccgc agcaccgagg gtgaagccac
     2041 cgccgggcgc agggatgtgg accaacccga gggcaactcc gacagcaagg ccgagggcga
     2101 acccaatggg gttgatgtcc gacaggccgc gctcgctgtc accgaggtag gcgctgacct
     2161 ccttgagccg accggccgga gcggtcaccc gcacccggtc gcccatctgc aggacgaagt
     2221 cttcgctggc caccatgtca aggtcgccgc gacgcacccg cgtggcgaca gcaccgaagc
     2281 gctcgtcgag ccccaactcg gcgatcgtcc ggcccgccaa cgctgcctgc gacacggtga
     2341 tccggcggaa gtcgaggtcg cgacgctggg actgcagcgc gtgcgaggac cgatggccca
     2401 ggcgctcggc gacgccgctc acggcacccg ccgggccgac gaccgtgacg aggtctcctg
     2461 cctgcagaat ctcgtgctcg tcgatcacct cgatggggtt ctcctcgccg tgctggatgc
     2521 gcgagaagac gacggtgccg ccactgtccc ggacgatctc gtgggcgcag atcggcgcgt
     2581 cgaggtcgac ccggatggtg aggttggtca gcggatcacg agcgcgatcg tcactgcttc
     2641 ggcgcaggac gagcatcgcg gcaacgagca tggcggcaac gccgtaaagg taggcgatgg
     2701 agtagccgat ggttggaccc gtcgtgtcgc ccgcgcgttc ggtcgctgca gcaagtgcgg
     2761 gtgtgttggt cagcgcaccg gcgaacgtcc cagcgatgac cgggctgctc aggccgagca
     2821 gccggcctcc gaggacgccc acgaacccga cgaccacgaa cacgccgacg accgcgacca
     2881 tcgccaccca gccgcggcgc agcgaggcga agaagttggg tcccgacagc aggcctaccg
     2941 tgtaggtaaa caggaccagc ccgagcgtcc ccaccacttc gggaagctcg agcttgagcc
     3001 cgtatgccgt cccaaggctg gagatcgcga tcgcgccgaa gaggaccgcc gccggcccga
     3061 ggtgaactcc cttatatcgg aaggatccca acagcgagcc gagccccaga aggatggcaa
     3121 gcagcaggat cggttgttgg gacagcaagc gaagcaggtc aagcacgtac tccacccttg
     3181 cacgtcgaag ggtcccagcg ccaaccgtgc agcgggccgc ggactgctgc ggacggcata
     3241 cccttgcctt tgtgagcatc gaaacggtga ccccgaagca cactcccctc ggcctgctcg
     3301 tcctggggca gggccgcgac ctgcacagcg aacgcggcgt cgaatgcccc ggcgacctgc
     3361 ccgccccgtc cgaccccgat ctggtccgac gcgcccgcgc ggccaaggcg gcgctcgggg
     3421 acaaggtgtt cgtgctcggg caccactatc agcgcgacga ggtcatcgag ttcgcggacg
     3481 tgaccggcga ctcgttcaag ctggcgcagc aggccgccgc ccgcccggag gcagagtgga
     3541 tcgtcttctg cggcgtgcac ttcatggccg aatcggccga catcctcacc accgatgccc
     3601 agacggtcgt gctccccgat ctggcggccg gctgctcgat ggccgacatg gccaccgcgg
     3661 cccaggtcga cgactgctgg gacgacctca tcgacgccgg ggtcgcggag cacaccatcc
     3721 ccgtgtcgta tatgaactcc tcggccgcga tcaaggcgtt cacgggtcgg cacggcggca
     3781 cgatctgcac gtcgtccaac gcccgcactg cgctggagtg ggcctttgag cagaccggcg
     3841 gagtcgaggg caccggcaag gtgttgttcc tgcccgacca gcacctgggt cgcaacacgg
     3901 cggtccggga gttgggtctg tcgctggacg actgcgtcgt ctacgacccg caccgccccg
     3961 gcggcgggct caccaaggag gccatgcagg ccgcccgagt catcctgtgg cgggggcact
     4021 gctcggtcca cggccggttc acggtcgacg cggtccaagc ggcccggcgc gagatccccg
     4081 gggtccgagt gatcgtccac cccgagtgcc agtacgaggt ctgcgaggcg gccgacgtca
     4141 tcggctcgac cgagcagatc atcaaggccg tggcggctgc accggccggg acctcctggg
     4201 tggtcggcac cgagctcaac ctggtcaagc gcctgtcgac catgttcccc gagcagtcga
     4261 tcgccttcct cgagaagaac gtctgctact gctcgacgat gaaccgcatc gacctgcccc
     4321 acctggtgtg gacgcttgag tcgctcgccg aggggcggat cgtcaacccg atccgggtcg
     4381 accccgagat cgcccactgg gc
//
LOCUS       DAORPW010000192         4391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6061_length_4391_cov_863.118542, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000192 DAORPW010000000
VERSION     DAORPW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6061_length_4391_cov_863.118542"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..970)
                     /locus_tag="PLB86_11540"
     CDS             complement(8..970)
                     /locus_tag="PLB86_11540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRC65817.1"
                     /translation="MGKIGWVHAWQEALYGPRGFYRGQVPARDFATATHPPLGAVLAE
                     AIWHYADLLELSGVVDIGAGRGELIGALHAHRPDRPLLGVDVVERPADLPDGVGWLRG
                     PGGAPLPDELSDLTDVLVVAHEWLDVVPCVIGQLDADGVVREVLVDPTSGDEDLGDPL
                     ADAELAWQQRHWPLLAEGDRVEVGLSRDLAWADLLSRVCRGAAIAIDSGHVAAARPRN
                     GTLAAYREDAAVRPVPDGSCDLSAQVAVDSLHHDEIHTQTTAFESFGVWAPMPDPALA
                     RCDETAYRRRLLRATSAATLTDPAGHGAFRWVVVRQAGAMTKRS"
     gene            1025..1798
                     /locus_tag="PLB86_11545"
     CDS             1025..1798
                     /locus_tag="PLB86_11545"
                     /inference="COORDINATES: protein motif:HMM:NF022187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRC65818.1"
                     /translation="MSTPTGPTARLRVGVIGAGRAGVALGAALARAGHLPVAVAAVSD
                     ASRERAEAVLPGVPIREALDVVAEADLVLLAVPDDVLPGLVAGLAAAGAFHPGQVLLH
                     TSARYGIGVLEPAATHDVLPVAIHPAVALTGELGDGERLAAALFAVTTLKSLRPLGEA
                     LVMEMGGEPVWVEEEDRPAYAAALGAVRDGLEGVLGAASRVLADCGIPHPARALAPLV
                     LTALEDALRTGSVARDPGVYAAPVVDRPSDGPEPLEDDQ"
     gene            1795..3519
                     /locus_tag="PLB86_11550"
     CDS             1795..3519
                     /locus_tag="PLB86_11550"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRC65819.1"
                     /translation="MTPIHIPRWLRAPSPGWSTSADVIVVGSGIAGLTAALRLRARGA
                     TVLLVTKTLLTEGSTAWAQGGIAAALADDDSPGDHLHDTLVAGVGLCDIPAVTALVHE
                     GPTRVRELVTLGAEFDRGSDGAISLTREGGHHRDRILHAGGDATGKEISRALIEALHR
                     VTDDPGIEIIEHALVVDLLQDAGGRVCGATIHVIGEGQIDGVGAAQARAVVLATGGLG
                     QIYSSTTNPPVATGDGMAIALRAGAVMADMEFVQFHPTVLFLGDDSTGQQPLISEAVR
                     GEGAFLVDGFGVRFMQGVHPMADLAPRDVVSRAIVRQMAATGSDHVFLDARHLGAEFL
                     ERRFPSIVASCREHGFDPATTLLPVAPAQHYASGGIRTDLVGRSSLDGLYACGECSCT
                     GVHGANRLASNSLLEGLVFAHRIAEDIAERFAHGELPSVTATAAAPQSDGPDGSGIGE
                     GRMRDTVLLRSVHRKAIQRAMTSGSGVVRSASTLESTASALATLAAEADGDEGGPKSW
                     ETTNLLHLGQLLTQVAALREETRGGHVRSDFVDRDDAHFLGHVRAVRAADGILATSFH
                     PTAPQDLS"
     gene            3516..>4391
                     /gene="nadC"
                     /locus_tag="PLB86_11555"
     CDS             3516..>4391
                     /gene="nadC"
                     /locus_tag="PLB86_11555"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRC65820.1"
                     /translation="MTPDLAFPAADARALVRLALDEDLGPRHVDVTTLSTIPADQERT
                     AHVVARAAGVIAGVPVVAEVFGAVADLIGCATPEVELLVADGTAVVRGDVVARVSGPV
                     RAILIGERTMLNIVSRLSGVATHTRRWADRLDGTGAMVLDTRKTTPGMRHLEKYAVRA
                     GGGTNKRMGLYDVAMIKDNHKIAAGGLAPAYAAVRRTFPEVDIQVEVTTVAEALEAYA
                     AGARFIMCDNMSVALLGETVAALRAQDEPVEIEATGGLTLEIARDYALTGVDYLSVGG
                     LTHSSPILDVALDLVD"
BASE COUNT          600 a         1561 c         1578 g          652 t
ORIGIN      
        1 tgcgcggcta cgaacgcttg gtcatcgcgc cggcctgacg cacgaccacc caccggaagg
       61 caccgtgacc agccggatcg gtgagcgtcg ccgccgaggt cgcccgaagc aaccgacgcc
      121 ggtatgccgt ctcgtcacat cgcgccaggg ccgggtcggg catcggcgcc cacaccccga
      181 acgactcgaa cgccgttgtc tgagtgtgga tctcgtcatg gtgaagggag tcgacggcga
      241 cctgcgcgga gaggtcgcaa ctgccgtctg gcacgggtcg cacggccgcg tcctcccggt
      301 atgccgcgag cgtcccgttc cggggccgcg ccgccgcgac gtgaccggag tcgatcgcga
      361 tggcggcgcc gcggcatacc cgagagagca ggtcagccca cgcgaggtca cgggagagac
      421 cgacctcgac ccggtcaccc tccgccaaca acggccaatg ccgttgctgc caggcgagtt
      481 ccgcgtcggc cagcggatcg ccgaggtcct cgtcaccgct ggtcgggtcg acgagcacct
      541 cgcggacgac accgtcagcg tcgagttggc cgatgacaca cgggacgacg tcgagccatt
      601 cgtgggccac gacgaggaca tcggtgaggt cgctcaactc gtcgggcagc ggcgcgccgc
      661 cgggcccacg caaccagccc accccgtcgg gcaggtcggc cgggcgttcg acgacatcga
      721 ccccgagcag gggacggtcc ggccgatggg cgtgcaacgc cccgatgagc tcaccacggc
      781 cagcaccgat gtcaacgacg ccggagagct cgagcaggtc ggcgtagtgc cagatcgcct
      841 cggcgaggac ggcgcccaga ggtggatggg tcgcggtggc gaagtctcgc gccggcacct
      901 gcccgcggta gaacccgcga gggccgtaga gcgcctcttg ccatgcgtgg acccagccga
      961 tcttgcccat agtcggaaac ctactcgcgc acgcccccac tccagaggag ggtaggttcg
     1021 gaggatgagc acgccaacgg ggccgacggc ccggcttcgc gtcggggtca ttggcgccgg
     1081 gcgcgccggc gtcgccctcg gagcagccct cgcccgcgcc gggcaccttc ccgtggctgt
     1141 cgccgcggtc agcgacgcct cccgcgagcg ggccgaggcg gtgctgcccg gggtgcccat
     1201 ccgtgaggcg ctcgacgtgg ttgccgaggc tgatctggtg ctcttggccg tccccgacga
     1261 cgtgctgccc gggttggttg ccgggctggc cgcggctggt gcctttcacc ccggacaggt
     1321 gctcctgcac acgagcgctc ggtatgggat cggcgtgctc gagcccgccg cgacccacga
     1381 cgtcctgccg gtggccatcc acccggcggt cgccctcacc ggtgagcttg gtgacggcga
     1441 acgcctcgcc gccgcgttgt tcgcggtgac cacgctcaag tcgctgcggc ccctcgggga
     1501 ggcgctcgtc atggagatgg gcggcgagcc cgtctgggtc gaggaagagg accgaccggc
     1561 gtatgccgcc gcgctgggtg ccgtccgcga cggtcttgag ggggtgctcg gggcggcctc
     1621 ccgcgtgctg gccgactgcg gcatacctca ccctgcccgt gcgctcgcgc cgctcgtcct
     1681 caccgcgctc gaggacgcgc tgcggaccgg tagcgttgcc cgcgatccgg gggtgtatgc
     1741 cgcgccggtg gtggatcggc ccagcgacgg gccagagccc ttggaggacg atcagtgacg
     1801 ccgatccaca tcccgcgctg gctgcgggcg ccctctccgg ggtggtcgac gtcggccgac
     1861 gtcatcgtcg tcgggtcggg gatcgcgggt ctcaccgcgg cactgcgact gcgcgcacgt
     1921 ggtgccacgg tgctgctggt caccaagacc ctgctcaccg aggggtccac cgcgtgggcc
     1981 cagggcggta tcgcggccgc gctggccgac gacgactccc caggcgacca cctgcatgac
     2041 acgctcgtcg caggcgtggg gctgtgcgac atccccgctg tgacagctct ggtccacgaa
     2101 gggccgacgc gggtccgcga attggttact ctcggtgcgg agttcgaccg ggggagcgac
     2161 ggggcgatca gcctgacccg ggaaggcggc caccatcgtg accgcatcct gcacgccggt
     2221 ggcgatgcga ccggcaagga gatctcgcgg gccctcatcg aggccctgca ccgcgtcacg
     2281 gatgacccgg gcatcgagat catcgagcac gccctcgtcg tcgacctgct ccaggacgcc
     2341 ggagggcggg tgtgcggggc gacgatccac gtcatcggcg aggggcagat cgacggggtc
     2401 ggtgccgcgc aggcccgcgc cgtcgtcctc gcgacgggcg gcctcggcca gatctactcc
     2461 tcgacgacca acccgccggt ggcgaccggt gacggcatgg cgatcgcctt gcgggccggg
     2521 gcggtcatgg ccgacatgga gttcgtccag ttccacccca cggtgctctt cctcggggac
     2581 gactcgaccg gccaacagcc gctcatctcc gaggcggtcc gcggtgaggg ggcgttcctc
     2641 gtcgatggct tcggggtgcg tttcatgcag ggcgtgcacc cgatggccga cctggccccg
     2701 cgcgatgtcg tgtcccgggc gatcgtgcgg cagatggctg cgacgggctc cgaccacgtc
     2761 ttcctcgatg cgcgtcacct gggtgccgag ttcctcgagc gacgcttccc gtcgatcgtt
     2821 gcgtcgtgcc gcgagcacgg cttcgatccg gcgacgacgc tgttgccggt ggctccggcg
     2881 cagcactatg ccagcggcgg gatccgcacc gacctggtcg gccggtcgtc gctcgacggg
     2941 ctctatgcct gcggcgagtg ctcctgcacc ggagttcacg gtgccaaccg cctggcgtcc
     3001 aactcgctcc tggagggcct ggtgttcgcc caccggatcg ccgaggacat cgccgagcgc
     3061 ttcgcccacg gcgagttgcc gtcggtcacg gcgacggccg ccgccccgca gtccgacggc
     3121 ccggacggtt caggcatcgg cgagggccgg atgcgcgaca ccgtgctgct gcgctcggtc
     3181 caccgcaagg ccatccaacg cgcgatgacc agcggttccg gggtggtgcg ctcggcctcg
     3241 acgctggaga gcaccgcttc ggccctggcg acgctggctg cggaggccga tggcgacgaa
     3301 ggcggcccca agtcgtggga gaccaccaac ttgttgcacc tgggccaact cctcacccag
     3361 gtcgccgccc tgcgtgagga gacacgtggg ggccatgtcc gcagcgactt cgtcgaccgc
     3421 gatgacgcgc acttcctcgg ccacgtccgc gcggtccggg cggcggacgg catactcgcg
     3481 acctccttcc accccaccgc gcctcaggat ctttcgtgac cccagacctc gccttcccgg
     3541 ctgccgatgc ccgtgccctc gtgcgcctcg ccctcgatga ggacctcggc ccgcgccacg
     3601 tcgatgtgac gacgttgtcg acgattcccg ccgatcagga gcgcacggcc cacgtcgtgg
     3661 cccgcgcggc cggagtgatc gcaggcgtgc cggtcgtcgc agaggtgttc ggggcggtcg
     3721 ccgacctcat cgggtgtgcc accccggagg tcgagttgct cgtcgcggat gggaccgcgg
     3781 tcgtgcgcgg ggacgtcgtg gctcgggtgt ccggcccggt ccgagccatc cttatcggag
     3841 agcgcacgat gctcaacatc gtctctcggc tctcgggggt cgccacccac acccggcgct
     3901 gggccgatcg gctcgatggc acgggggcga tggtcctcga cacgcgcaag acgacgccgg
     3961 ggatgcgtca cctggagaag tacgccgtgc gcgccggcgg aggcaccaac aagcggatgg
     4021 gcctctacga cgtcgcgatg atcaaggaca accacaagat cgcggcgggt gggctcgcgc
     4081 cggcgtatgc cgcggtgcgc cgcaccttcc ccgaggtcga catccaggtc gaggtcacca
     4141 ccgtcgcgga ggctctcgag gcgtatgccg cgggcgcccg attcatcatg tgcgacaaca
     4201 tgtcggtcgc gctgttgggt gagacggtgg cggccctgcg ggcgcaggat gagccggtgg
     4261 agatcgaggc gaccggtggg ctgacgctgg agatcgcccg ggactatgcc ctgaccgggg
     4321 tcgactatct gtcggtcggc gggctgaccc actcgtcgcc gatcctcgac gtggccctcg
     4381 acctcgtcga c
//
LOCUS       DAORPW010000193         4390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6065_length_4390_cov_451.394464, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000193 DAORPW010000000
VERSION     DAORPW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6065_length_4390_cov_451.394464"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLB86_11560"
     CDS             <1..440
                     /locus_tag="PLB86_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC65821.1"
                     /translation="PDQMSGGQQQRVAIARALVGDRRLVLADEPTGALDSLTGEGVLA
                     VLRARCDAGAAGLLVTHEARHAAWADRVVYLRDGRVVDSTEAYSAASMPRARHTPAPS
                     GTSPSPESGASAGMPPAPGPSAAVTPLGDTASVGAHSESGREG"
     gene            444..3482
                     /locus_tag="PLB86_11565"
     CDS             444..3482
                     /locus_tag="PLB86_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRC65822.1"
                     /translation="MATITTPPLAPAPADPSRDGVGEWRAGWRVALRLARRDVRRHVG
                     RSLLIVLMVAVPVLLLVGGNIVFSSQAVSSVERIPYAMGQTQAIAAYYGGAVVAPQVD
                     PWAPYYGGSSTKDERAKPIPGWGTDPIANAQALGALVGATAIPIAQGNGFTVIGRRQV
                     EVLLRGIDGGRFAAQAQGIARLESGHWPTSSTEAVVTKVGVYHGLPTSGTLTVTAAAG
                     TVTTYTIVGVGEGWIANYDLRAADLITLPTADAQEGGEAAYLIDRPAPVTWDEVKRLA
                     TYGLQMTSRQVILNPPSREELALPDGVQMMADDAAAGQAVTSAIMAIGLLLETTLLVG
                     PAFAVSAARQRRTLALAASNGATTAQLRRTVLAQALVLGVLAALVGAVVGVLGAWAVI
                     WWSRTYRPDTVFGPFDVPVLPVAIIVGCAVVSAVIAAMIPSRGLGRLDIVAVMRGQSV
                     SPPALVRTPIAGIVLAGLGAVGVFWAVAFTSGAERTWIDDVVPLVAMGGAIMLVIGAL
                     LLVPMVLVLAGRVARSAPVAIRMALRDAARQRGRATSTVAAILGGTALLSAILVVAAS
                     DTAYRAKTYVPQLPMGQALLSPTTAMDGSLADPRWTQNVTDIAHGIDQALQVREVAVV
                     DASRTSGAVQKPSGPGSPMVMPFFVALRTGCTPAQALQMGAPTDLNSAPDTGCLSLSG
                     MGMGDARSAILVGTVAQLVQTYTLDATAEATLRAGGMVVVGDKAVTPTWTKYPEGGWG
                     ASGPILGQVDIVDGRVSFARGTMTVTHGADGGIALTDLATLSLPATPVPAATLNATTA
                     GMAGPSYGGMQMVGALMTLETAKSLALPVATIELMIVDPSGPLSATDEAALTSAVSDS
                     NLGWFYVERGFQPYDTVLAIIVLSFIGLIILVATLVSTALSTAETQSMMGTFAAVGAT
                     RLTRRNLAAAQAGSLGVIGALLGTLVGFVPGIALARAYTRYPMEAMTYGEYTAPALVD
                     PTVVIPWLQLAIPVIAVPALAALLAWLAIRKAPTVTRRLT"
     gene            3633..4250
                     /locus_tag="PLB86_11570"
     CDS             3633..4250
                     /locus_tag="PLB86_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial proteasome activator family protein"
                     /protein_id="HRC65823.1"
                     /translation="MSDQPETSGSAPDENRIVVITPDGMGVASSGHGDGEGHDSRDSR
                     EVTNPADLVEQPAKVMRIGSMIKNLLDEVRNAPLDEAGRLRLAEIHRRSIDELKDGLA
                     PELVEELDRIALPFGAGAPSDAELRIAQAQLVGWLEGLFHGIQTALVAQQMAAQQQLA
                     GMRRALPPGHPDAGPAGPGMPPGFPGAPGQGQGEQGHPGPTGQYL"
BASE COUNT          624 a         1561 c         1542 g          663 t
ORIGIN      
        1 accccgacca gatgtccggc gggcagcagc agcgcgtcgc catcgcccgc gcgcttgtcg
       61 gcgaccggcg tttggtcctc gccgacgaac ccaccggtgc gctcgactcg ctcaccggcg
      121 agggcgtgct cgcggtgctg cgggcccgct gcgacgcggg cgcggccggg ctgctcgtca
      181 cccacgaggc acggcacgcc gcctgggccg accgggtcgt ctacctgcgc gacggtcggg
      241 tcgtcgactc caccgaggcc tacagcgccg cgagtatgcc gcgcgcgcgg catacccctg
      301 cgccgtccgg cacgtcgcct tcgcccgagt cgggcgcttc tgcgggtatg ccgcccgccc
      361 ccggccccag cgcggccgtc acgcctctcg gggataccgc gtcggtcggc gcccacagcg
      421 agtccgggcg cgagggctga gcgatggcca ccatcaccac tcctcccctg gcccctgccc
      481 ccgcagaccc gagccgcgac ggtgtcggcg agtggcgggc cggctggcgg gtcgccctgc
      541 gactggccag gcgcgacgtg cgacgccacg tcgggcgcag cctgctcatc gttctcatgg
      601 tggccgtgcc tgtgctgctg ctggtcggcg gcaacatcgt cttctccagc caggctgtgt
      661 ccagcgtcga gcggatccct tacgccatgg gccagaccca ggccatcgct gcctactacg
      721 gcggcgccgt ggtggccccg caggtcgatc cgtgggcccc ctactacggc gggagctcga
      781 ccaaggacga acgggccaag cccatcccgg gctggggcac cgatccgatc gcgaacgccc
      841 aggccctcgg agcgctcgtc ggagcgacgg cgattccgat cgcgcaaggc aatggcttca
      901 cggtgatcgg gcgtcgccag gtggaggtgc tcctgcgcgg gatcgacggg ggccggttcg
      961 cggcccaggc ccagggcatc gcccggctgg aatcgggcca ctggccgacc tccagcacgg
     1021 aggccgtcgt caccaaggtc ggcgtctacc acgggctgcc cacgagcggc acgctcaccg
     1081 tgaccgccgc ggccgggacc gtcaccacct acacgatcgt cggagtcggc gaagggtgga
     1141 tcgccaacta cgaccttcgg gccgccgacc tcatcaccct gcccaccgca gacgcccagg
     1201 agggcggcga agcggcatac ctcatcgacc ggcccgcccc cgtgacgtgg gacgaggtga
     1261 agcggttggc gacctacggc ctgcagatga ccagccgcca ggtgatcctc aacccgccgt
     1321 cgcgcgagga gttggcgctt cccgatgggg tgcagatgat ggcggacgat gcggcggccg
     1381 gtcaggcggt gacgtcggcg atcatggcca tcgggctgct gctggagaca acgctgctcg
     1441 tcgggccggc ctttgcggtg agtgcggccc ggcagcgacg cactctcgcc ctcgcggcga
     1501 gtaacggggc gacgactgcg cagttgcgcc ggaccgtact ggcccaggcg ctggtgctcg
     1561 gcgtcttggc cgcccttgtc ggtgccgttg tcggtgtgct cggcgcgtgg gcggtgatct
     1621 ggtggtcgcg gacctatcgg cccgacacgg tcttcggccc gttcgacgtg ccggtcctgc
     1681 cggtcgcgat catcgtcggg tgcgccgtgg tgtccgccgt catcgccgcg atgatcccct
     1741 ctcgggggct cgggcggttg gacatcgtcg cggtgatgcg tggccaaagc gtgtcgccac
     1801 cagcgctcgt gcgcacgccc atcgcgggca tcgtgctcgc cgggctgggg gccgttgggg
     1861 tcttctgggc ggtggcgttc acctccggcg ccgagcggac ctggatcgac gatgtcgtgc
     1921 cgctggtcgc gatgggtgga gcgatcatgc tcgtcattgg cgccctcctg ctggtgccga
     1981 tggtgctcgt gctcgccggc cgcgtcgccc ggtccgcgcc cgtggccatc cggatggcgt
     2041 tgcgcgacgc cgcgcggcag cggggaaggg ctacctcgac ggtggcggcg atcctcggcg
     2101 ggaccgccct gttgtcggcg atcctcgtcg tggccgccag cgacacggcc tatcgagcga
     2161 agacctacgt gccccagttg cccatgggtc aggccctgct cagccccacg accgcgatgg
     2221 acggcagcct ggcggaccca cggtggaccc agaacgtcac tgacatcgcc cacgggatcg
     2281 accaggccct gcaggtccgc gaagtcgcgg tcgtcgacgc gagtcgcacc agcggcgccg
     2341 tccagaagcc gagtgggcct gggtccccga tggtcatgcc gttcttcgtg gccctgcgga
     2401 ccggttgcac cccggcacag gctttgcaga tgggggctcc aaccgacctc aacagcgcac
     2461 ccgatacggg ttgcctctcg ctcagcggca tgggaatggg cgatgcacgg tccgccatcc
     2521 ttgtgggaac cgtcgctcaa ctcgtccaga cctacacgct ggacgcaacc gccgaggcga
     2581 ccttgcgggc cggcgggatg gtcgtagtgg gcgacaaagc ggtgaccccg acctggacga
     2641 agtaccccga gggcggctgg ggcgcctccg gcccgatcct gggccaggtg gacatcgtgg
     2701 acggacgagt ctcgtttgcc cgaggcacga tgacggtgac ccacggcgct gacggcggga
     2761 tagcgctgac ggacctggcg accctgtcgt tgcctgcgac cccggtgccg gccgcgaccc
     2821 tcaacgcgac caccgccggg atggccggcc cgagctacgg cgggatgcag atggtcggtg
     2881 cgctcatgac gctcgagacg gcgaagtcgc tggcgttgcc ggtggcgacc atcgagctga
     2941 tgatcgtcga cccgagcggc ccactcagcg ccaccgacga ggcggccctc acctcggccg
     3001 tgagcgatag caacctcggc tggttctatg tcgagcgggg cttccagccc tacgacacgg
     3061 tgttggcgat catcgtcctg tcgttcatcg gcctgatcat cctcgtggcg accctcgtct
     3121 cgacggcgct gtcgacggcc gaaactcagt cgatgatggg caccttcgcg gcggtcggtg
     3181 cgacgcgatt gacccgacgc aatctcgctg cagcgcaggc aggttcgctg ggcgtcatcg
     3241 gcgcgctact gggcacgctc gtgggtttcg tgccggggat cgccctcgct cgggcataca
     3301 cgcgctatcc gatggaagcg atgacctatg gcgagtacac cgcacccgcc ctcgtggatc
     3361 cgacggtcgt catcccctgg ctgcagttgg ccatcccagt catcgcggtc cctgccctgg
     3421 ccgcgctgtt ggcctggctg gcgatccgca aggccccgac ggtgacccgc cgcctgacct
     3481 gagcgcttct gcacgcaacc gatcggccga cggcgacgtc tcaaccgaca caggggatcg
     3541 acctccgcgg acgcgtgggc ggcatacaca gggggtatgc cgcccaccga gtcccggagc
     3601 ccccgtcggg ccgagtgcgg caagatggag ccatgagcga ccagcctgag acctctggat
     3661 cggcccccga cgagaaccgg atcgtcgtca tcacgcccga tgggatgggc gtcgcgtcct
     3721 cggggcacgg cgacggtgag ggtcacgact cacgcgactc gcgcgaggtg accaacccgg
     3781 ccgacctggt cgagcagccg gccaaggtca tgcggatcgg ctcgatgatc aagaacctcc
     3841 tcgacgaggt gcgcaacgcc ccgttggacg aggcgggtcg gctgcggctg gccgagattc
     3901 accgccgctc gatcgacgaa ctcaaggacg gtttggcgcc ggagttggtc gaggaactcg
     3961 accggatcgc gctgccgttc ggtgcgggag caccgagcga cgccgagttg cggatcgcgc
     4021 aggctcagct tgtcggctgg ctcgagggcc tgttccacgg gatccagacc gcgttggtcg
     4081 cccagcagat ggccgcccaa cagcagttgg ccgggatgcg ccgcgcgctg ccgccgggac
     4141 accccgacgc tggcccagca ggccccggta tgccgcccgg cttccccggc gcgcctggcc
     4201 aaggccaagg cgaacaaggc caccccggcc caaccggcca gtacctctaa ccggatttgg
     4261 tgacgtgcgt catgccaaac gcgacgtatg ccgcgcgtct cgcatgacgc actcgccggt
     4321 cagaccttgc gggctaggac gtaaccgaaa gcgaccgaac gaacgccttc gtcaacaatg
     4381 gtgaacccgg
//
LOCUS       DAORPW010000194         4376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6101_length_4376_cov_764.496644, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000194 DAORPW010000000
VERSION     DAORPW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6101_length_4376_cov_764.496644"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..440
                     /locus_tag="PLB86_11575"
     CDS             66..440
                     /locus_tag="PLB86_11575"
                     /inference="COORDINATES: protein motif:HMM:NF021525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HRC65824.1"
                     /translation="MSAWTASTLAAFATVEEVRIVTQRQDGSLRRPRIIWVVALSDRL
                     FIRSVNGRDADWFRWAIATGTGQLEASGTAYDVTFTEAQEGDLDAVDAAYQGKYGRRY
                     ASIVRDLCRPVPRAATLQVHLA"
     gene            complement(501..2057)
                     /locus_tag="PLB86_11580"
     CDS             complement(501..2057)
                     /locus_tag="PLB86_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC65825.1"
                     /translation="MPRAERSGAAYPLGYMLERRTRPDRPLGQPKEAAAMFFTQYYLD
                     CLSQASYLVGDASSGLAVVVDPRRDVADYLADARAAGLRIVGVINTHFHADFVSGHLE
                     LAAETGAWIGYGEAATPEFTHRPLADGERISLGDVTLQIMATPGHTPESISVLVFERA
                     DDDVAYGVLTGDALFIGDVGRPDLLASVGVSADALGRMLYDSVQHKLMGLADEVRVFP
                     AHGAGSACGKNLSTEKQSTIGAQRALNYACQPMTEDEFLAVVTEGQPSAPAYFLYNAT
                     LNKQERATRGLDAVISALDDEAVAAALADGAVVHDARDAVAFTAGHLRGAVSVPADGR
                     LSETAGMVFRPDQPIVVMAPEGQEQEIALRLARIGFDRVVGYVADVEAYLVAHADQVT
                     QASRLTVAQADTALAAQSAAQPAAGGTVAAGPAAAGPAAAGGVQLVDIRNPGETAGGT
                     IPGARTIPLPELPRRLGELDATAPVIVYCAGAWRSSVAASLLRANGFVDVSDILGGYN
                     AWRQAHPIAG"
     gene            2132..2749
                     /locus_tag="PLB86_11585"
     CDS             2132..2749
                     /locus_tag="PLB86_11585"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC65826.1"
                     /translation="MTHELRDHWDRAYALGDHTRSWFQEEAAPSVAAFDAAGVTPADS
                     VIDVGGGASRLVEGLLDLGFDDVTVLDVSPAAIQIARTRAGLRAARVTWLVTDLLEWI
                     PTRTYAVWHDRAVLHFLVTDEDQRRYAAALDRATEPGSVAVIAGFAPDGPTQCSGLPT
                     ARHDEVSLAALVGPKWELASSGREVHRTPNDAEQSFLWTTFRRIG"
     assembly_gap    2797..2896
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2944..>4376
                     /locus_tag="PLB86_11590"
     CDS             2944..>4376
                     /locus_tag="PLB86_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65827.1"
                     /translation="MPGWYVHMEAAHDTARSMRNGTIPPGFPISPAEAMDIGNHCHTW
                     RNYLALGSLGPDLFYLLPDFKNTTGQVIRQVVQWCLDVWEVVDSEFVSKWEKWIDPIS
                     TNNSQLASQLTGGLSTQLAQVLDELTSAILSAFKGLLAQMGDWFGVLTSGVPQGFGDD
                     AFYWSDMFHYRKTYQFPFVLFQQAQAALASATTDDERADAQARIAFAVGWMSHCATDV
                     TGHPYTNAKSGGPYRDHWQRHHLVENHMDSENYVARHPGSLYAEYGTSALHFRLAFRH
                     RNDAPYNGRDDAPAYDYWTGFPAYDNGDGPTPTANRHTFFDLDTGPLPGHLVDGLLEA
                     MADVYGVDGPHILMQDPPFSATDPGTGQPDGRPNEPAMAQMWEITYRYLKMTSSDAIS
                     LRLPPPPSVFTDHSFPTPPGGGGSGIDDDPSRGADVDDDDSFNLLDLLLALFAWIVYL
                     AQVVVWLLTILPGLILDITTFPAREVI"
BASE COUNT          677 a         1557 c         1383 g          659 t
ORIGIN      
        1 caaggctgct ccgcgtaggc tcgtggatca gcccgcagct caagcccgga cgagcaggag
       61 gtcccatgtc agcgtggacg gcatcgacgc tcgcggcgtt cgccaccgtt gaagaggtgc
      121 gcatcgtgac gcagcgacag gacggaagcc tgcgacgccc gcggatcatc tgggtggtgg
      181 cgctgagcga ccggctgttc atcaggtcgg tcaacgggcg cgacgccgac tggttccgat
      241 gggcgatcgc cacgggtacc ggccaactcg aggcctcggg cacggcatac gacgtgacct
      301 tcaccgaggc ccaggagggc gacctcgatg cggtcgacgc ggcataccaa gggaagtatg
      361 ggcgtcgcta cgcctccatc gtgagggact tgtgtcggcc cgtgccgagg gcagcgaccc
      421 tgcaggtgca cctcgcctga ctgagatccg gcggtgacac cggcgtcgat ccgtggcgat
      481 gctgagccgg cggaccgggc tcagccagcg atggggtgag cctgacgcca cgcgttgtag
      541 ccgccaagga tgtctgacac gtcgacaaaa ccgttggcgc gcaagagact tgccgcaacg
      601 ctggaccgcc aggcgccagc gcagtagacg atgacgggcg ccgtcgcgtc gagctcaccc
      661 agccgccgag gcagctcggg caacgggatc gtccgggcgc ccgggatcgt gccgccggcc
      721 gtctcgccgg ggttgcggat gtcgaccagt tgaaccccac cggctgcggc cgggccagcc
      781 gccgccgggc cagcggcgac tgttcccccc gcagccggct gggcagccga ctgggcagcc
      841 agagccgtgt cggcctgggc gaccgtgagc cggctcgcct gcgtgacctg gtcggcgtgg
      901 gccaccaggt aggcctcgac atcggcgaca tagcccacca cgcgatcgaa accgatccgg
      961 gccagacgca acgcgatctc ctgctcctga ccctcgggcg ccatgacgac gatcggctga
     1021 tcgggccgga acaccatgcc ggcggtctcg gacagccgac cgtcggccgg cacgctgacc
     1081 gctccacgaa ggtgcccggc cgtgaaagcg accgcgtcac gggcatcgtg caccacggct
     1141 ccatcggcca gagcggccgc cacagcctca tcatcgaggg ccgagatcac ggcgtcaagc
     1201 ccgcgggtgg cgcgctcctg cttgttgagc gtcgcgttgt agaggaagta cgccggagcg
     1261 ctcggctggc cctcggtgac gacggcgagg aactcgtcct ccgtcatggg ttggcaggcg
     1321 tagttcagcg cccgttgggc gccgatggtc gactgcttct cggtggagag gttcttaccg
     1381 cacgcggagc cggcgccgtg ggccgggaac acccgcacct catcggccaa ccccatcagc
     1441 ttgtgctgca ccgagtcgta gagcatccgc ccgagcgcgt cggcgctcac cccgaccgac
     1501 gcgagcaggt cgggccggcc gacatcgccg atgaacagcg cgtccccggt aagcaccccg
     1561 taggccacgt cgtcgtcggc gcgctcgaac accaggacgc tgatcgactc gggggtgtga
     1621 ccgggggtgg ccatgatctg cagggtcacg tcaccgaggc tgatccgctc tccgtcggcc
     1681 agtggtcggt gcgtgaactc cggggtggcc gcctcgccgt agccgatcca tgcgccagtc
     1741 tcggccgcca gctccagatg ccccgagacg aagtcggcgt ggaagtgcgt gttgatgacc
     1801 cccacgatgc ggagccctgc cgcccgggcg tcggcgagat agtcggccac atcgcggcgc
     1861 ggatccacga cgacggccag gccgctcgac gcgtcgccca cgaggtagga cgcctgcgac
     1921 aagcaatcaa ggtagtactg cgtgaagaac atcgcggcgg cctccttcgg ctggcccagc
     1981 ggtcggtcgg ggcgggtgcg ccgctccagc atatacccca gggggtatgc cgcgccagat
     2041 cgttccgcgc gcggcatacc cagcctgtcc gcggcatacc catcctgccc ggggcatacc
     2101 caacggcctc tcgcggggct aacctgcaga tgtgacgcac gagttgcgtg atcactggga
     2161 ccgggcctac gcgctcggtg accacacgcg cagttggttc caggaggagg ccgccccctc
     2221 ggtggccgcc ttcgacgcgg ccggagtgac accggccgat tcggtcatcg acgtcggtgg
     2281 cggtgcctcc cgactcgtcg agggcctgct cgatctcggc ttcgacgacg tcacggtcct
     2341 tgacgtctcg ccggccgcca tccagatcgc ccgcacgcgt gccggtctgc gcgcggctcg
     2401 ggtcacctgg ctggtcaccg acctgctcga atggatcccg acccgcacgt atgccgtgtg
     2461 gcacgaccgc gcggtcctgc acttcctcgt caccgacgag gatcaacgcc gctatgccgc
     2521 cgcgctggat cgcgccaccg agccgggcag cgtggccgtc atcgccggtt tcgcccccga
     2581 cggcccgact cagtgctccg ggctgcccac ggctcggcac gacgaggtct ccctggccgc
     2641 gcttgtcggg ccgaaatggg agttggcctc cagcgggcgc gaagttcatc gcactccgaa
     2701 cgatgccgag cagtccttcc tctggaccac tttccgccgc atcggctagc cggcgtatct
     2761 ggccggcata ccgggactcc ttcactccat cagggcnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnngcac tctcccgcac tctcccgcac ggttccgctt cagaggagtc
     2941 accatgcccg gttggtacgt ccacatggaa gcggcccacg acaccgcccg atcgatgcgg
     3001 aacggcacga tccctccggg ctttcccatc agccccgccg aggcgatgga catcggcaac
     3061 cactgccata cctggcgcaa ttacctcgcg ctcggctccc tcgggccgga cctcttctac
     3121 ctgctgcccg acttcaagaa cacgactggc caggtgatcc gccaggtcgt ccagtggtgc
     3181 ctcgacgtct gggaggtcgt cgacagcgag ttcgtcagca agtgggagaa gtggatcgac
     3241 ccgatctcga cgaacaactc ccaactcgcc tcccagctca ccgggggcct ctcgacccag
     3301 ctcgcccagg ttctcgacga gctcacctcg gcgatcttgt ccgcgttcaa ggggctgctc
     3361 gcgcagatgg gcgactggtt cggcgtgctg acctccggtg tgccgcaagg gtttggcgac
     3421 gacgccttct attggtcgga catgttccac taccgcaaga cctaccagtt cccgttcgtc
     3481 ctcttccagc aggcgcaggc cgcgctcgcg tccgccacca ccgacgacga gcgcgccgac
     3541 gcccaggccc ggatcgcctt cgcggtcggc tggatgtcgc actgcgccac cgacgtcact
     3601 ggccacccct acaccaacgc caagtccggc ggcccctacc gcgaccactg gcagcgtcac
     3661 cacctcgtcg agaaccacat ggactccgag aactacgtgg ctcgccaccc cggctccctg
     3721 tatgccgagt acggcacctc cgcgctgcac ttccggctgg cgttccggca ccgaaacgac
     3781 gccccctaca acggccggga cgacgcaccc gcctacgact actggaccgg cttcccggcc
     3841 tacgacaacg gtgacggacc cacaccgacg gcgaaccggc ataccttctt cgatctggac
     3901 accgggccct tgccggggca tctcgtcgac ggactgctcg aggcgatggc cgatgtctat
     3961 ggggtcgacg ggccacacat cctcatgcag gacccgccgt tctcggcgac cgacccgggg
     4021 accgggcagc ccgacggtcg cccgaacgag ccggcaatgg cgcagatgtg ggagatcacc
     4081 taccgctacc tcaagatgac cagcagcgat gcgatcagcc tgcggctgcc gccgccgccc
     4141 tcggtgttca ctgaccactc gttcccgacg cctcctggcg ggggcgggtc ggggatcgac
     4201 gacgacccca gccgcggcgc cgacgtcgac gatgacgact ccttcaacct gttggacctg
     4261 ctcctcgccc tgttcgcgtg gatcgtctat ctcgcccagg tcgtcgtctg gctgctgacg
     4321 atcctgcccg gcctcatcct cgacatcacg accttcccgg cccgcgaagt catcta
//
LOCUS       DAORPW010000195         4337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6194_length_4337_cov_799.373657, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000195 DAORPW010000000
VERSION     DAORPW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6194_length_4337_cov_799.373657"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLB86_11595"
     CDS             <1..630
                     /locus_tag="PLB86_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRC65828.1"
                     /translation="VGSSNPIRDLDLMLVPYRPGEHRLVVANRGLAGIDGTVSTAIGV
                     ALGRAGRIPPPGRGSPPSPDDGGIPRPGRSLAVMGDLTFLHDSGGLLIGPDEPRPDLT
                     IVVVNDDGGSIFALLEQGTPDYAAAFERVFATPVHADLEALAGASHTAYRRVESRREL
                     REALTEPATGLLVIEARVGRADRRETAAALGALAADVPLSWPPDQGGMP"
     gene            627..2411
                     /locus_tag="PLB86_11600"
     CDS             627..2411
                     /locus_tag="PLB86_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRC65829.1"
                     /translation="MSRPLWVVGADGSVVSRALGVLRLSLLALAIGQGLVANAFATAS
                     LLPYLISLVVTGGILGSTLLPQLLPQFVTSDREDPAGDADKTGDADKSDDVQVAPVDE
                     AGSTAPAPNGGANDAAPSLRFVQRLGTRLDIDRGLAIILGGLLLLTIACIVGASTLVR
                     GLGSGLTGSLGDLTASFAAVTLVQVFLYGVCAVFGRVLAARRRLGALGWAPAIGELVG
                     IGGLLAFMRLFQGHVSSAEWTPEMVWWLAGAATAGIAVQALVLAFAVWRSGPGTATLV
                     AAWSVSRLFGGRSVTALVTVASYAVAAQVMWRATGYDEVSRGPGQTFVAGLASLVNAL
                     VVVLLPHALLTRPALGSVHQRVAAALQADDRAAAGRRFASGLAVPAVVTIPGSIALVV
                     FALPIMGLLFTSKDPAEVLAGSTVLACLGPALFPMGIALLGRRVCEAVGAHWSAGALT
                     AVLALTSIGVSVGAGFAPPEWTTPLVALGLVLGNTLAAAYGVWVVRRRIGTVALAGVV
                     RTWVRILLAGAGGGYVAWGLVMVLTRALGGLGRLGYALTLLIGGTAFIVVYLLVARLL
                     RVDEVSVVAAPLVNRLDHRPPGRHRPGD"
     gene            complement(2545..3561)
                     /locus_tag="PLB86_11605"
     CDS             complement(2545..3561)
                     /locus_tag="PLB86_11605"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HRC65830.1"
                     /translation="MPPLASSVGLPPLGELLDGLVVVRISLRVRFRGVIEREVALLRG
                     PRGWGEFGPFLEYGPRESSRWLACAIEAAYAGWPAPVRSAVPVNATVPAVAADSVAGV
                     LARFPGCTTAKVKVAEAGQGLADDLDRVAAVREILGPDGRIRVDANGGWDVASAQRAL
                     GALAAYRLEYAEQPCAEVSDLVVLRTLLARNGIDVPIAADESIRKAADPMRVAREGAA
                     DLIVVKVAPLGGVRRALEIVADCGLPAVVSSALDTSVGMAAGVALAAALPELPWACGL
                     GTLALLEGDVVADPAVQHGGVLSVSAEPPQVDEALVARWSAEPERVAWWTERVAAAYA
                     HLLA"
     gene            3661..>4337
                     /locus_tag="PLB86_11610"
     CDS             3661..>4337
                     /locus_tag="PLB86_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRC65831.1"
                     /translation="MSTGALPFVSATFAADAWAPVAGFELTDITYHRCVLPGPARGTV
                     RIAFDRPEVLNAFRPHTVDELYRVLDHARMTPDVGCVLLTGNGPSPREGSSPTTEGWA
                     FCTGGDQRIRGRSGYQYAADPDGDATAQGIDARRVAAEGGRLHILEVQRLIRTMPKVV
                     IAVVNGWAAGGGHSLHVVCDLTIASREHARFKQTDADVGSFDGGYGSAYLAKMVGQKF
                     AREIFFLG"
BASE COUNT          573 a         1671 c         1459 g          634 t
ORIGIN      
        1 gtcggctcgt ccaacccgat ccgcgacctg gacctcatgc tcgtccccta tcggccaggc
       61 gaacaccggc tcgtcgtcgc caaccggggc ctggccggca tcgacggcac ggtctcgacc
      121 gccatcgggg tcgcgctggg ccgggccggc cgcattcccc cgccgggtcg aggtagtccc
      181 ccatcccctg acgatggcgg aatacctcga ccgggcagga gcctggccgt catgggcgac
      241 ctgaccttcc tgcacgacag cggggggctg ctcatcggtc cggacgaacc ccgtccggac
      301 ctgacgatcg tcgtcgtcaa cgacgacggc ggctcgatct tcgcgctgct cgagcagggc
      361 acccctgact acgcagccgc cttcgagcgg gtgttcgcga cgccggtgca cgccgatctg
      421 gaggcgctcg cgggcgccag ccacacggca taccgacggg tcgagtctcg ccgcgagctc
      481 cgggaggcgc tcaccgagcc agccaccgga ctcctggtca tcgaggcgcg ggtggggcgc
      541 gcggaccggc gcgagaccgc tgccgcgctc ggggcactcg ctgccgacgt gccgttgtcg
      601 tggccgcccg accaaggggg tatgccgtga gccgcccctt gtgggtcgtc ggggcggacg
      661 ggtcggtcgt gtcccgggcg ttgggcgtcc ttcgactctc actcctggca ctggccatcg
      721 ggcagggtct ggtggccaac gcgttcgcca cggcgagcct gctgccctat ctcatctcgc
      781 tggtcgtcac cggcggcatc ctcggctcga cgttgttgcc ccagctcctg ccgcagttcg
      841 tcacgtccga ccgggaggac ccggctggcg acgcagacaa gaccggcgac gcagacaagt
      901 ccgacgacgt ccaggtcgcg cctgtcgacg aagccggctc gaccgccccg gcaccaaacg
      961 gtggtgccaa tgacgcggcg ccttccctcc gcttcgtcca acggctgggg acccgcctgg
     1021 acatcgaccg agggctggcg atcatcctcg gcgggctgct gctgctgacg atcgcctgca
     1081 tcgtgggggc gagcaccctc gtgcgcggac tgggcagcgg gctcaccggc agcctcggcg
     1141 atctgaccgc ctcgttcgca gcggtcaccc tggtccaggt cttcctctac ggcgtctgcg
     1201 ccgtcttcgg ccgggtgctc gccgcccgtc ggcggctcgg cgcgttgggc tgggctcccg
     1261 ccattggcga actcgtcggc atcggcgggc tgctggcctt catgcgactc ttccaggggc
     1321 acgtctcctc cgcggagtgg acccccgaga tggtgtggtg gctcgcgggt gcggccaccg
     1381 cgggaatcgc cgtgcaggcg ttggtgctcg cgttcgccgt gtggcgcagc gggccgggca
     1441 cggccaccct cgtggctgcc tggagcgtgt cgcggctgtt cggggggcgc tcggtgacgg
     1501 ccctggtgac cgtggcgagt tacgccgtcg cagcccaggt catgtggcga gccacgggct
     1561 acgacgaggt ctcccgcggg ccgggtcaga cctttgtggc gggtctggcg agcctcgtca
     1621 acgccttggt ggtcgtccta ctgccgcatg cgctgctcac ccgcccggca ctcgggtcgg
     1681 tgcatcagcg cgtggccgca gcactgcagg ccgacgaccg ggccgccgcc ggccgccgtt
     1741 tcgcctcggg tctcgcagtg cctgcggtcg tcaccatccc cggatcgatc gccctcgtgg
     1801 tcttcgccct gccgatcatg gggctgctgt tcacgtcgaa ggacccggcg gaggttctcg
     1861 ctggatccac cgtcctggcc tgcctaggtc cggcgctgtt ccccatgggc atcgccctgc
     1921 tgggccggcg cgtctgcgaa gccgtgggtg cgcactggtc ggccggggct ctgacggccg
     1981 tgctcgcact gacctcgatc ggcgtcagcg tcggggccgg gttcgcgccg cccgagtgga
     2041 cgactcccct ggtggcgctc gggctcgtgc tcggcaacac cctcgcggcg gcatacgggg
     2101 tctgggtcgt ccgccgccgg atcgggacgg tcgctctggc gggcgtcgtc cgcacctggg
     2161 tgcggatcct cctggccggc gcgggtggcg gctatgtggc ctgggggttg gtcatggtcc
     2221 tcacccgggc gctgggcggg ctgggccgcc tcgggtatgc cttgacgctc ctcatcggag
     2281 gcaccgcctt catcgtcgtc tacctgttgg tggcccgcct gctgcgggtc gacgaggtgt
     2341 ccgtcgtcgc tgcgcccctg gtcaaccggc tcgatcatcg gccccccggc cggcatcgcc
     2401 ccggcgactg atccgccggg gaccgcccct cgcagctgag ggcgcgacgg agtgcgatcg
     2461 acggggtgat caccccacca aacgcacacc gtcccgccct cgactcgcct gcgtgagatg
     2521 ccagcccgtc gccacccagc cgactcaggc cagcaggtgc gcgtatgccg ccgccacccg
     2581 ttcggtccac cacgcgaccc gctccggctc ggccgaccac cgcgccacca gggcttcatc
     2641 aacctgcggc ggctcggccg acaccgacaa gactcccccg tgctgcaccg ccggatcagc
     2701 cacgacgtca ccctcaagca acgccagcgt ccccagccca cacgcccaag gcagttcggg
     2761 caacgccgca gccagcgcca ccccagccgc catgccaacc gacgtgtcga gcgccgacga
     2821 caccaccgcg ggcagcccac aatcagccac gatctccagc gcccgacgca ccccgcccaa
     2881 cggcgcgacc ttgacgacga tgagatccgc cgccccctca cgagccaccc gcatcgggtc
     2941 ggccgccttg cgaatcgact cgtcagcggc gatgggcacg tcgattccgt tacgcgccaa
     3001 caaagtccgc aacaccacga gatcagagac ctccgcgcac ggctgctcgg catactccag
     3061 ccggtatgcc gcgagcgcac cgagcgcccg ctgcgcggac gccacgtccc acccgccatt
     3121 cgcgtcgacc cggatccggc cgtcgggccc gaggatctcg cgcaccgccg ccactcggtc
     3181 gagatcgtcg gccagcccct ggccagcctc agccaccttg accttggccg tcgtgcaccc
     3241 ggggaatcgc gccaacactc cagccaccga gtcggccgcc accgccggaa ctgtcgcatt
     3301 gaccggcacc gccgagcgga cgggcgccgg ccacccggca tacgccgcct cgatcgcgca
     3361 ggccagccaa cgggaggatt cgcgcgggcc gtattccagg aacgggccga actcacccca
     3421 cccgcggggt ccgcgaagta gcgccacctc acgctcaatg acgccgcgga agcgcactcg
     3481 cagggagatc cggacgacga cgagaccatc aagcagctcg cccagcggcg gcaacccgac
     3541 ggagctggcg agcggcggca tacctgcgag tgtgccagcc cgcgcctccc ccacgggagg
     3601 ccaggccgcc gcgatggact cgacgggcgg ggggtgcaag cgaggccata tccttcgggg
     3661 gtgagcaccg gcgccctccc cttcgtctcg gcgaccttcg ccgccgacgc gtgggccccg
     3721 gttgcgggtt tcgagctcac cgacatcacc taccaccggt gcgtgctccc cggcccggct
     3781 cgcggcaccg tccgcatcgc cttcgaccgg cccgaggtgc tcaacgcctt ccggccgcac
     3841 accgtcgacg agctctaccg ggtgctcgac cacgcgcgga tgacccccga cgtcggctgc
     3901 gtcctgctca ccggcaacgg cccgagcccc cgcgagggct cctcgccgac caccgagggc
     3961 tgggcgttct gcaccggcgg tgaccaacgc atccggggcc gctcgggcta ccaatacgcc
     4021 gccgaccccg atggcgacgc caccgcgcag ggcatcgacg cccgacgcgt cgccgccgag
     4081 ggcgggcgcc tgcacatcct cgaggtccag cggctcatcc gcaccatgcc caaggtcgtc
     4141 atcgcggtcg tcaacggctg ggcggccggc ggtgggcaca gcctgcacgt cgtctgcgat
     4201 ctgacgatcg ccagccgcga gcacgcccgg ttcaagcaga ccgacgccga cgtggggtcg
     4261 ttcgacggcg gctacggctc ggcctacctc gccaagatgg tcggccagaa gttcgcccgc
     4321 gagatcttct tcctcgg
//
LOCUS       DAORPW010000196         4329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6214_length_4329_cov_901.663781, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000196 DAORPW010000000
VERSION     DAORPW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6214_length_4329_cov_901.663781"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..812)
                     /locus_tag="PLB86_11615"
     CDS             complement(9..812)
                     /locus_tag="PLB86_11615"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRC65832.1"
                     /translation="MSTGRDGWRLAVGTLTTIPVRPPSVVDRAVAGRAMLLAPLAALP
                     LALIPLLAHTAIRWLGMPPLLAAALTVAALALANRGLHVDGLADTADGLAASYDRDRA
                     LEVMRRGDVGPAGVATVVLVLLIQVVALADLLQLPSWDRPLLAALAVAASRHTLAWVC
                     GRAFPAARPEGLGAAVAGSVPSGAAAAAGLALAAVGAVAISVLAPTDTPWSSVGWYAA
                     PVTIAVAVVAALAVALRARSRLGGVTGDVLGAGVEISLAAALTVAVCLG"
     gene            complement(809..1345)
                     /locus_tag="PLB86_11620"
     CDS             complement(809..1345)
                     /locus_tag="PLB86_11620"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRC65833.1"
                     /translation="MSVTVVTGGVRSGKSRHAEALLAERDDVTYIATGQPADADDAEW
                     RERVARHQAARPATWTTVETLDLVPLLGMPGGPLLVECLGTWVTGLVDRAGLWDDLPA
                     AAALVEAEGAALAEALTRTSRDVVLVTNEVGWSLVPLTPSGRFFTDALGRVNTAVAGV
                     ADHAVLVVLGRALTLGSL"
     gene            1385..2452
                     /gene="cobT"
                     /locus_tag="PLB86_11625"
     CDS             1385..2452
                     /gene="cobT"
                     /locus_tag="PLB86_11625"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRC65834.1"
                     /translation="MTIALSSLLATGVPPLDAAATAAADARQAQLTKPAGSMGRLESL
                     GSRLAGMYAVCPPPIPEPAVVAVFAGDHGVHAQGVSPWPQEVTVQMVANVAAGGAVVN
                     ALARQVGASVCVIDVGVAGALPPGVDAVGARIVDGTADFTTGPALTREQAEAAVLVGA
                     QTADRLIDEGARLLVTGDLGIGNTTASAALIAVFAGADPGAVTGPGAGSDARMLAHKI
                     AVIRAGIERHQPDPADPIGVLAAFGGAEHAALAGFLVAAAARRVPVILDGVIAGAAAL
                     AAAALCPGVTAYLLAGHRSTEPGATAALAALRLEPILDLGMRLGEGSGGVLAVPVVQA
                     AARLLREVATFDSAGVAGRED"
     gene            complement(2850..3614)
                     /locus_tag="PLB86_11630"
     CDS             complement(2850..3614)
                     /locus_tag="PLB86_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC65835.1"
                     /translation="MRITDSTVALVTGGASGLGGATVRRLVADGAKAVVLDLPSSPGA
                     AFAAELGDAVRFVPTDVRDEAQVQAAVDAARELGELRIAVNCAGVATPGRVIGRGGPL
                     SLDTFRSVVEINLIGTFNVLRLAANAMLANEPVDGDRGVVVMTASIAAYDGQVGQAAY
                     AASKGGIVGLTLSAARDLADKAIRVVTVAPGTFETPMMAGLPGETKAILEKQVPHPSR
                     LGNPAEYAALVRHIVENPMLNGEVIRLDGALRMPPR"
     gene            complement(3621..>4329)
                     /locus_tag="PLB86_11635"
     CDS             complement(3621..>4329)
                     /locus_tag="PLB86_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC65836.1"
                     /translation="GWRIDGTKAWISHAGHANYYTTFARIPDLGAKGITCFLVPDGTP
                     GLSFGKPEDKMGLHGDPVAQVIFDGVHLGAHHLIGAEGQGMAIALHALDNGRLGIAAA
                     ATGLAQSALDAATAYAMERRQFGKAIIEFQGLAFLLADMEAAVTSARAAYLYAARLKD
                     AGRPYTKEASVAKLVATDAAMKVTTDAVQVFGGYGYTRDFPVERYMREAKVTQIFEGT
                     NQIQRLVISRQLQRERG"
BASE COUNT          582 a         1495 c         1647 g          605 t
ORIGIN      
        1 tcgcacgttc agccgaggca gaccgccacg gtgagcgccg ccgccagcga gatctcgacc
       61 ccggcaccga gcacgtcgcc cgtcacgccg ccgagtcgtg agcgagcccg cagcgccacc
      121 gccagcgccg cgacgacagc caccgcgatg gtgacggggg cggcatacca gccgaccgac
      181 gaccacggcg tgtcggtggg ggcgaggacg gagatcgcga cggcccccac cgcggccagc
      241 gcaagccccg ccgcagccgc cgcacccgag gggacgctcc cggccacggc cgcgccgagg
      301 ccttcggggc gagccgccgg gaaggcgcgc ccacagaccc acgcgagggt atgccgtgac
      361 gccgccaccg cgagcgccgc cagcaggggc cggtcccaac tcggcaactg cagcaggtcg
      421 gcgagggcga cgacctggat gagcaggacg agcacgaccg tcgccacccc ggccggaccg
      481 acgtcgcccc ggcgcatgac ctcaagcgcc cggtcccggt cgtaggaggc agccaagccg
      541 tcggccgtat cagccagccc atcgacgtgc aggccccggt tggccagcgc gagcgcagcg
      601 acagtgagtg cggcggcgag caggggcggc ataccgagcc agcggattgc tgtgtgcgcg
      661 aggaggggga tgagcgccag tggcagggcg gcgaggggag cgagcagcat ggcgcgaccg
      721 gccacggccc gatcgacgac cgacggtggc cggaccggga tggtcgtcag ggtgcccacg
      781 gcgagccgcc agccgtcgcg gccggtgctc acagcgaccc caacgtcagc gcgcggccca
      841 gcacgacgag cacagcgtgg tcggcgaccc ccgctacggc cgtgttgact cgcccgagag
      901 catcggtgaa gaaccggccc gacggcgtga gcgggacaag ggaccaaccg acctcgttgg
      961 tgacgaggac gacgtcgcgc gacgtgcggg tgagggcctc ggccagcgcc gcgccctcgg
     1021 cctcgacgag ggccgccgcc gcggggaggt cgtcccagag gccggcccgg tcgaccaggc
     1081 cggtgaccca cgtgccgaga cattcgacga gaaggggacc acccggcata ccgaggagtg
     1141 ggaccagatc gagggtctcg acggtcgtcc aggtcgcggg gcgggcggct tgatggcgcg
     1201 cgacccgctc gcgccactcg gcgtcgtcgg cgtcggcggg ctggccggtg gcgatatagg
     1261 tgacgtcgtc gcgctcggcg agcagcgctt cggcatggcg gctcttgccg ctgcgcaccc
     1321 cacccgtcac gactgtcacc gacacgcgac caggctatgc ccgacgtcgg gcaggatgga
     1381 tcttgtgacc attgctctga gctccctcct cgccacgggt gtcccgccgc tggacgccgc
     1441 cgctaccgcc gccgccgatg cgcgtcaggc gcaactcacc aaaccggccg gctcgatggg
     1501 gcggctcgag tcgctggggt cacggctggc cgggatgtat gccgtgtgcc caccccccat
     1561 ccccgaaccg gccgtcgtcg cggtcttcgc cggcgaccac ggggtgcacg cccaaggggt
     1621 gtcgccgtgg ccgcaggagg tgaccgtgca gatggtcgcc aacgtcgccg cgggcggggc
     1681 ggtcgtcaac gcgttggccc gtcaggtggg ggcgagcgtc tgtgtcatcg acgtcggcgt
     1741 cgccggggcg ttgccgcccg gggtcgacgc ggtcggtgcg cggatcgttg acggcacggc
     1801 cgacttcacg acggggccgg ccctgacccg cgagcaggcg gaggctgccg tgctcgtcgg
     1861 cgcccagacc gctgaccggc tcatcgacga gggcgctcgg ttgttggtca ccggcgacct
     1921 ggggatcggc aacaccaccg cctcggctgc gctcatcgcg gtcttcgccg gtgccgaccc
     1981 gggcgcggtc acgggcccag gcgcgggcag cgatgcccga atgctggccc acaagatcgc
     2041 cgtcatccgg gcggggatcg agcggcacca gcccgatccc gccgacccga tcggtgtcct
     2101 ggccgccttc ggcggtgctg agcacgccgc gctcgcgggc ttcctcgtcg cggctgcggc
     2161 tcggcgggtg ccggtgatcc tcgacggcgt gatcgccggg gcggctgcgc tggcggcggc
     2221 ggcgctgtgt cccggggtga cggcatacct gctggcgggg catcgctcca cggagccggg
     2281 ggcgacggcg gcgctggccg cgctgcggct cgagccgatc ctcgacctgg gcatgcggtt
     2341 gggcgagggg tccggcggcg tgttggccgt gccggtcgtc caggcggccg cgcggctgtt
     2401 gcgtgaggtg gccacgtttg actcggccgg ggtcgccggt cgcgaagact gaccctcgcg
     2461 gggtgcgggc tggccggggt gattctcgtg acacgccgga ccgaagtccc actctcgcgg
     2521 gctgtggcgc ggtagaaagg gtgaggcccc accaagtatg agttggtggg gcctcgtgcg
     2581 tccgtgccgt gacgccgact cgaactcgtc cagcgcgcct gatcctccgg tgtcgtccta
     2641 ccggcccgct tgggattgct cccggggatt gctcccgcgg cgtggaacct cgtttgccgt
     2701 ccgaccgctt ccgcctttcg gcttctgcgt agggataggt ctacgccgtt gggaggggct
     2761 tcgcaagggt tctggcgaag ttttgtcgaa gtaatttcgg gcttttcggc gtgtcgcctc
     2821 gacacgcgca ggtatgccgt tcggtcgggt cagcgagggg gcatccgcag ggcgccgtcg
     2881 agccggatga cctcgccgtt gagcatgggg ttctcgacga tgtggcggac gagggcggca
     2941 tactcggccg ggttgcccag ccgcgagggg tgcgggacct gcttctcgag gatcgccttg
     3001 gtctccccgg gcaggccggc catcatcggc gtctcgaagg tgcccggtgc gacggtgacg
     3061 acgcgaatcg ccttgtctgc caagtccctt gccgccgaca gggtgaggcc gacgatgccg
     3121 cccttgctcg cggcatacgc ggcctgaccg acctgaccgt cgtaggcggc gatgctcgcg
     3181 gtcatgacga ccaccccgcg gtcgccgtcg acgggctcgt tggcgagcat cgcgttggcg
     3241 gccaggcgca gcacgttgaa ggtgccgatg aggttgatct cgacgaccga gcggaaggtg
     3301 tcgagcgaca gcgggccgcc gcgaccgatc acccggccgg gagtcgcgac gccggcgcag
     3361 ttgacggcga tccgcagctc gccgagctcg cgcgcggcgt cgacggccgc ctgcacttgc
     3421 gcctcgtcgc gaacgtcggt gggcacgaag cgcaccgcgt cgccgagctc ggccgcgaag
     3481 gcggcgccgg gggagctggg caggtccagg acgacggcct tggcgccgtc ggcgaccagg
     3541 cgccggacgg tcgctccgcc gaggccggac gcccctccgg tgacgagggc gacggtggaa
     3601 tcggtgatgc gcatgcggga tcagcctctc tcgcgctgca gctggcggga gatgaccagc
     3661 cgctggatct ggttggtgcc ttcgaagatc tgggtgacct tggcctcgcg catgtagcgc
     3721 tcgacgggga agtcacgggt gtagccgtag ccgccgaaca cctggacggc atcggtggtc
     3781 accttcatcg ccgcgtcggt cgccacgagc ttcgcgaccg acgcctcctt cgtgtagggg
     3841 cggcccgcgt ccttgagccg cgcggcatac aggtaggccg cccgcgccga cgtcaccgcc
     3901 gcctccatgt cggcgaggag gaacgcgagc ccctggaact cgatgatcgc cttcccgaac
     3961 tggcgacgtt ccatcgcgta tgccgtcgcc gcgtcgagcg cggactgggc caggcccgtc
     4021 gcggcggcag cgatcccgag gcgtccgttg tcgagggcgt gcagggcgat cgccatgcct
     4081 tggccttcgg cgccgatcag gtgatgggcg ccgaggtgga cgccgtcgaa gatcacctgg
     4141 gcgaccgggt cgccgtgcag ccccatcttg tcctcgggct tgccgaacga caggcccggt
     4201 gtgccgtcgg ggacgaggaa gcaggtgatg cccttggcgc cgaggtcggg gatccgggcg
     4261 aaggtcgtgt agtagttggc gtgccccgca tgggagatcc acgccttggt gccgtcgatt
     4321 cgccagccg
//
LOCUS       DAORPW010000197         4300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6277_length_4300_cov_570.383746, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000197 DAORPW010000000
VERSION     DAORPW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6277_length_4300_cov_570.383746"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /gene="recO"
                     /locus_tag="PLB86_11640"
     CDS             complement(<1..724)
                     /gene="recO"
                     /locus_tag="PLB86_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRC65837.1"
                     /translation="MPLYRDEAVVLRTHQLGEADRIITALTRSRGKIRTVAKGVRRTS
                     SRFGARLEPFMVVDLQCYEGRNLDTVTQAETLAPHGDAICRDYTAYTAATAVVETCDR
                     LTEEGEPATQQYLLVAGAMAALAAGEHDPGLVLDAYLLRALAVAGWAPSFHDCAKCGA
                     PGPHRAFNVAAGGCVCLVCRPPGSASPSPDTMRLLSALLTGDWVLADGSSTGHRREGS
                     GLVAAFLQWHLERGVRSLRLVER"
     gene            complement(752..1801)
                     /locus_tag="PLB86_11645"
     CDS             complement(752..1801)
                     /locus_tag="PLB86_11645"
                     /inference="COORDINATES: protein motif:HMM:NF025393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="questin oxidase family protein"
                     /protein_id="HRC65838.1"
                     /translation="MMTGEGNYDEALERFHATGPEFQGWLSNHGPMVIEAMDRRGAGG
                     RIHRWTDDYLDRLEDRPRSLFPIGPDEWRDPLGDPARTGDWIEYFLKEIREQPWSDVV
                     ALWWPRLLPGIAAGATHGVIRVGHALRSIREEDTQPRRDEVAHALAYWAARWQPVPVV
                     SAAGQRRPADLDDLIDAIPAIPDQRFGIRFRLRQLGDAAGFAATAAQLARPTQTADVP
                     GALAQVVDAAVAAYPRLAHGNATMLVHAATAPNAVLLALPSLPERLWPDSFDAAWTAT
                     AAVVAAYRPAEPARVPQPRDGADDSWAPDSWERAVADGGEHIVKLADTALSAVERTGD
                     LIGLAAIDTAIRLEA"
     gene            1842..2288
                     /gene="soxR"
                     /locus_tag="PLB86_11650"
     CDS             1842..2288
                     /gene="soxR"
                     /locus_tag="PLB86_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012246428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HRC65839.1"
                     /translation="MDSRDLLTIGEVAHRSGLAPSALRYYESLGLIRSHRTSGNQRRY
                     TRSVLRRLAVVHAAQQVGLSLEEVCRAFAAFDPEHAPTKREWVALSRRWRPALDARIK
                     ELEQVRDGLSMCVGCGCLTMRQCAIYNPQDALGDTGSGARRIFPTQ"
     gene            2403..3035
                     /locus_tag="PLB86_11655"
     CDS             2403..3035
                     /locus_tag="PLB86_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HRC65840.1"
                     /translation="MSLSVQGSLLDLDDHPAFGELGDTVQRITLGLGAWLDVRPGWLT
                     GSDTLFEQVRTAVPWQAERRQMYDNVVDVPRLTRFYRTGEALPLALLDDARDRLSAHY
                     RPELGEDFTSAGLCLYRDGNDSVAWHGDTIGRGATTDTMVAILSLGAARPLCLRPRGG
                     GRTAYRIPLGHGDLAVMGGSCQRTWEHAIPKTAKQVGARISVQFRPRGVA"
     gene            complement(3123..>4300)
                     /locus_tag="PLB86_11660"
     CDS             complement(3123..>4300)
                     /locus_tag="PLB86_11660"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRC65841.1"
                     /translation="LEFAVRVCNEVIDVIDPTPDHKMIINLPATVEMITPNVYADSIE
                     WMVRHLDRRDSVVVSLHPHNDRGTGVAAAELGYLAGADRIEGCLFGNGERTGNVDLVT
                     LGMNLFSQGIDPQIDFSNIDEIRRTVEHCNQLPVHERHPYGGDLVFTAFSGSHQDAIK
                     KGFEWLEKDAAAAGTDVNGVPWAVPYLPIDPHDLGRSYEAVVRVNSQSGKGGVAYIMK
                     TEQGMDLPRRLQIEFSAVVQRDVDQDGGEVTPADLWHKFEDEYLPTPEHPWGRFRLAS
                     AKVEADEDGETLLTLGIRDRSASATEVMTLTGHGNGPIAAFCEAINQLGVDVRVLDYA
                     EHAMSSGGDATAAAYVECAVGERVLWGVGIDSSIVRASLKAIVSAVNRALRETTVLV"
BASE COUNT          628 a         1508 c         1495 g          669 t
ORIGIN      
        1 tgcgctccac gagacgcaag gatcgcacac cgcgttccag gtgccactgc aggaaggccg
       61 cgaccaggcc gctgccctct cgccggtgtc cggtgctgga cccgtcggca aggacccagt
      121 cgccggtgag cagcgccgac agcaaccgca tcgtgtccgg cgagggcgag gcggagccgg
      181 gcgggcggca taccaggcag acgcagccgc cggcggcgac gttgaaggcg cgatgcgggc
      241 cgggggcacc gcacttggcg cagtcgtgga aactcggcgc ccagcccgcg acggcgagcg
      301 cccgcagcag gtaggcgtcg agcaccagcc ccgggtcgtg ctcgccggcc gcgagcgcgg
      361 ccatcgcgcc ggcgacgagg aggtattgct gggttgccgg ctcgccctcc tcggtcagcc
      421 ggtcgcaagt ctcgacgacg gccgtcgccg cggtgtatgc cgtgtagtcc cggcagatcg
      481 catcgccatg gggcgcaagg gtttcggcct gggtgacggt gtcgaggttg cggccttcgt
      541 agcactgcag gtcgaccacc atgaagggct ccagccgcgc cccgaagcgc gacgacgtgc
      601 gccgcacccc tttggcgacg gtgcggatct tgccgcgcga gcgggtgagc gccgtgatga
      661 tccggtcggc ctcgcccaac tggtgggtgc gcaggacgac cgcctcgtca cggtagagcg
      721 gcaccaggcc attgtccctt gtcgcgggga ctcaggcctc caggcggatc gccgtgtcga
      781 tggcggccaa cccgatcagg tcgcccgtgc gctcgacggc cgatagcgcc gtgtcggcga
      841 gtttgacgat gtgctcgccg ccgtcggcca ccgcccgttc ccacgagtcg ggtgcccacg
      901 agtcgtctgc cccgtcgcgc ggctggggaa cccgcgcggg ctcggccggt cgataggccg
      961 cgacgaccgc ggccgtcgct gtccacgccg cgtcgaaact gtccggccac aggcgttcgg
     1021 gcaggctcgg caaggccaag aggacggcgt tgggtgccgt cgccgcgtgg acgagcatcg
     1081 tcgcgttccc atgcgccagc cgcgggtatg ccgcgaccgc cgcgtccacg acctgcgcca
     1141 aggcgccggg cacgtctgcc gtctgagtcg gccgcgcgag ttgggcggcc gtcgccgcga
     1201 agcctgcagc gtcgccgagc tgacgcaacc ggaagcggat cccgaaccgc tggtccggga
     1261 tggcggggat tgcgtcgatc aggtcgtcca ggtcggccgg ccggcgttgt cccgccgccg
     1321 acaccaccgg aaccggctgc caacgcgccg cccaataggc caaggcgtgc gccacctcgt
     1381 cacggcgcgg ctgcgtgtcc tcctcacgga tcgatcgcaa cgcgtgcccc acccggatca
     1441 cgccgtgggt tgcccccgca gcgatgcccg gaagcagtcg tggccaccac agggccacca
     1501 cgtctgacca tggctgttcg cgaatctcct tgaggaagta ttcgatccag tcgccggtcc
     1561 gagccgggtc gcccagtggg tcgcgccact cgtcgggccc gatgggaaag agggatcgcg
     1621 gccggtcctc gagccggtcg agataatcgt ccgtccagcg gtggatccgc ccgcccgcac
     1681 cccgccggtc catcgcctcg atcaccatcg ggccatggtt ggacaaccat ccctggaact
     1741 cgggcccggt cgcgtggaag cgctcaagcg cctcgtcgta gttgccttcg cctgtcatca
     1801 tgagtccatt ccaaaacatc aagtgcactt gaggtcaagc cgtggattcc cgtgatcttc
     1861 tgacgatcgg tgaggtggcg caccgcagcg gcctggcccc ttcggcgctg cgctactacg
     1921 agtccctcgg gctcatccgc agccaccgca ccagcggcaa ccagcgccgc tacacccgct
     1981 cggtgttgcg ccggttggcc gtcgtccacg cggcgcagca ggtgggcctg tccctggagg
     2041 aggtatgccg cgccttcgcg gccttcgatc ccgagcacgc accgaccaag cgcgaatggg
     2101 tcgccctctc ccgacggtgg cggccggccc tcgatgcgcg gatcaaagaa ctcgagcagg
     2161 tgcgtgacgg gctgtcgatg tgcgtcgggt gcggctgcct gacgatgcgt cagtgtgcga
     2221 tctacaaccc gcaagatgcc ctcggcgaca ccggcagcgg tgcccgacgc atcttcccga
     2281 cccaatagcc gactcgtagg tgagcagaac cgtcgctggg acgtcggttc tgctcacctg
     2341 accgctcagc cccacgcggc cgccttccca ccttactcgt acacatgttc tataatggcc
     2401 tcatgtctct cagcgtccag ggctccctgc tcgacctcga cgaccacccg gcgttcggtg
     2461 agttgggcga cacggtccag cgcatcacgc tcggcctcgg cgcctggctc gacgtccgcc
     2521 caggctggct cacgggatcg gacaccctct tcgagcaagt gcggacggcc gttccgtggc
     2581 aggccgagcg acgccagatg tacgacaacg tcgtcgacgt cccccgtctc acccgcttct
     2641 accgcaccgg ggaagcgctc cccctggcct tgctcgatga cgcccgcgac cgactctcgg
     2701 cgcactatcg gcccgagttg ggcgaagact tcaccagcgc cgggctgtgc ctctatcggg
     2761 atggcaacga cagcgtggcc tggcacggcg acaccatcgg ccgtggcgcg acgaccgaca
     2821 cgatggtcgc gatcctgtcc ctcggcgccg ctcgcccgct gtgtctgcgc ccgcgcggag
     2881 gtgggcggac ggcataccgg atcccgctcg ggcacggcga cctcgcagtg atgggcggct
     2941 cctgccaacg cacctgggag cacgcgatcc cgaagaccgc caagcaggtg ggcgcgcgga
     3001 tctcggtcca attccgcccg cgcggcgtcg cctgagatcc cgccccccca cacaggggta
     3061 tgccgccctc tcacggtgag cgggcggcat acctgatgtg cgtggtgaga gcgcgagaag
     3121 cctcagacga ggacggtggt ctcgcgcagg gcccggttaa cggccgagac gatggccttg
     3181 agcgaggcgc ggacgatcga ggagtcgatg ccgacgcccc agaggacccg ctcgccgact
     3241 gcgcactcga cgtaggcggc cgccgtggcg tcgccgccgg aggacatggc gtgctcggcg
     3301 tagtcgagga ctcgcacgtc gacgcccaac tggttgatcg cctcgcagaa cgcggcgatc
     3361 gggccgttgc cgtggccggt cagggtcatc acctcggtgg ccgacgcgct gcggtcgcgg
     3421 atgccgaggg tgagcagggt ctcgccgtcc tcgtcggcct caaccttggc cgaggcgagc
     3481 cggaagcggc cccacgggtg ctcgggcgtc gggaggtact cgtcctcgaa cttgtgccac
     3541 aggtcggcgg gcgtgacctc gccgccgtcc tggtcgacgt cgcgctggac gactgcggag
     3601 aactcgatct gcagccggcg cggcaggtcc atgccctgct cggtcttcat gatgtaggcg
     3661 acgccgccct tgcccgactg gctgttgacg cgcacgaccg cctcatagct gcggccgagg
     3721 tcgtgcgggt cgatcggcag gtagggcacc gcccacggca cgccgttgac gtcggtgcct
     3781 gcggcagcgg cgtccttctc cagccactcg aagcccttct tgatggcgtc ctggtgcgag
     3841 ccggagaagg ccgtgaagac gaggtcgccg ccgtaggggt ggcgctcgtg caccggcagt
     3901 tggttgcagt gctcgacggt gcgacggatc tcgtcgatgt tcgagaagtc gatctgcggg
     3961 tcgatgccct gcgagaagag gttcatcccg agggtgacca ggtcgacgtt tccggtgcgt
     4021 tcgccgttgc cgaacaggca gccctcgatg cggtcggcgc cggcgaggta gcccaactcc
     4081 gccgccgcga ccccggtccc ccggtcgttg tgcgggtgca gcgacacgac gaccgagtcg
     4141 cgacggtcca ggtgtcggac catccactcg atggagtcgg catagacgtt cggggtgatc
     4201 atctcgacgg tcgcggggag gttgatgatc atcttgtggt cgggggtcgg gtcgatgacg
     4261 tcgatgacct cgttgcacac ccggacggcg aactccaact
//
LOCUS       DAORPW010000198         4288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6302_length_4288_cov_843.253957, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000198 DAORPW010000000
VERSION     DAORPW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6302_length_4288_cov_843.253957"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /gene="glgX"
                     /locus_tag="PLB86_11665"
     CDS             complement(<1..1423)
                     /gene="glgX"
                     /locus_tag="PLB86_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRC65842.1"
                     /translation="MQIWPGKPYPLGATYGGSGVNFALFSEVAERVELCLISDDGTET
                     RVEMPEHDAFVWHVYLPTIQPGQRYGYRVHGPYDPAKGHRCNPDKLLLDPYATAIDGE
                     MQGSQALFGYSFGDPALGLSSVPNAVDSLGHTMYSVVTNPFFDWGKDRPPGHAYHDSV
                     IYEAHVKGLTMLHPDVPKEIRGTYAGIGHPATIEHLRRLGVTAIELMPVHQFVQDFTL
                     LDKGLRNYWGYNTIGFLAPHNGYAAYGTRGQQVLEFKTMVKALHEADIEVILDVVYNH
                     TAEGNHLGPTLAFRGIDNDNYYRLVGGDLAHYYDTTGTGNSLLMRSPHVLQLIMDSLR
                     YWVTEMHVDGFRFDLAATLARQFHEVDKLSAFFDLIQQDPIVSQVKLIAEPWDLGDGG
                     YQVGGFPPLWTEWNGKFRDTVRDFWRGEAATLGEFASRFTGSSDLYAHSGRRPVASVN
                     FVVAHDGFTMRDLVSYNDKHNDAN"
     gene            1621..2196
                     /locus_tag="PLB86_11670"
     CDS             1621..2196
                     /locus_tag="PLB86_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65843.1"
                     /translation="MGTLRNILILTAIAALSACAGPATSPPSSASTSPSSLGGFENSP
                     ASTTPLLDPAQLAVEAFLRTDPEVVRRFGATPHDGRLACAFAPIVPRRDNHLFARFLC
                     LEVMGTGPGAYSISGSASSIAMIIDPASGAVRDWRTPRDGADGRTDLLEMFPADVMNA
                     FRSADTATANRQTSEMQSRCLAIANELAAAS"
     assembly_gap    2206..2305
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2406..3368)
                     /gene="meaB"
                     /locus_tag="PLB86_11675"
     CDS             complement(2406..3368)
                     /gene="meaB"
                     /locus_tag="PLB86_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRC65844.1"
                     /translation="MPPRPVDVPALVTAARAGSPRAVARLISLVEDAHPALREVMAAL
                     APHTGKAHVIGLTGSPGVGKSTSTSALVAAFRARGKRVGILAVDPSSPFSGGALLGDR
                     VRMQEHALDPEVYIRSMASRGHLGGLAWTTPQAIRVLDAAGCEVVLVETVGVGQSEVE
                     IAGLADTTIVLLAPGMGDGIQAAKAGILEIGDVFVVNKADRDGADATVRDLRHMISLG
                     DRTEPGLWRPPVCKTVASRGEGLDEVMEAIDKHEAWLDATGTRHTRRLRRASDEIEAI
                     ALAALRARMGDLRAGHGIAEHAAAVVAGTTDPYAAADDVVAHLA"
     gene            complement(3506..>4288)
                     /locus_tag="PLB86_11680"
     CDS             complement(3506..>4288)
                     /locus_tag="PLB86_11680"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRC65845.1"
                     /translation="VKMRDHMDYDALWDVYTDQGMGNLTESANSGDREFTREEQDAFS
                     ARSHELAAKAWKDGLFDDEVVPVMIPQRKGDPIEFKADEGVRAATTAESLAGLRPAFR
                     KDGTITAGSASQISDGAAAVVVMSKAKAEALGLTWLAEIGAAGYVAGPDSTLQSQPAH
                     AIVKACAKEGITPADLDLLELNEAFAAVGLASTKELGVDPDKVNVNGGAIAMGHPVGM
                     SGARIVLHLANELKRRGGGVGAAALCGGGGQGDALIVRVPKA"
BASE COUNT          650 a         1456 c         1439 g          643 t
ORIGIN      
        1 cattggcgtc gttgtgcttg tcgttgtagg acaccaggtc ccgcatggtg aagccgtcgt
       61 gcgcaacgac gaagttgacc gacgcgaccg gccgacgacc ggagtgggca tagaggtcag
      121 acgacccggt gaaccgcgag gcgaactccc ccagggtcgc cgcctcacca cgccagaagt
      181 cgcgcaccgt gtcgcggaac ttgccgttcc actcggtcca cagcggcggg aagccaccca
      241 cctggtaacc accgtcgccg aggtcccacg gctcggcgat gagcttgacc tggctgacga
      301 tcgggtcctg ctggatgagg tcgaagaacg ccgagagctt gtcgacctcg tggaactgcc
      361 gcgccaacgt cgccgcgagg tcgaaccgga acccgtcgac gtgcatctcg gtcacccagt
      421 agcgcagcga gtccatgatg agttgcagga cgtgcgggct gcgcatgagc aggctgttcc
      481 cggtgcccgt tgtgtcgtag tagtgcgcga ggtcgccgcc gaccagtcgg taatagttgt
      541 cgttgtcgat cccccggaag gccaacgtcg ggcccaggtg gttgccctcg gcggtgtggt
      601 tgtagacgac gtcgaggatc acctcgatgt cggcctcgtg cagcgccttg accatcgtct
      661 tgaactccag cacctgctgc ccgcgggtgc cgtaggcggc atacccgttg tgaggggcga
      721 ggaagccgat ggtgttgtag ccccagtagt tgcgtaatcc cttgtccagc aacgtgaaat
      781 cctggacgaa ctggtgcacc ggcatgagct cgatcgccgt cacgcctagg cggcgcaggt
      841 gctcgatcgt cgcggggtgg ccgatgccgg catacgtccc gcggatctcc ttgggcacgt
      901 cggggtggag catcgtcagc cccttgacgt gcgcctcata gatgaccgag tcgtgatagg
      961 cgtgccccgg cgggcggtcc ttgccccagt cgaagaacgg gttggtcacg acggagtaca
     1021 tcgtgtggcc gagggagtcg acggcgttgg ggacgctcga caggcccaac gccgggtcgc
     1081 cgaaggagta gccgaacagc gcctggctgc cctgcatctc cccgtcgatc gccgtggcgt
     1141 aggggtcgag caagagcttg tccgggttgc accggtgacc cttcgcgggg tcgtaggggc
     1201 cgtggacgcg gtagccgtag cgctgaccgg gctggatcgt cggcaggtac acgtgccaca
     1261 cgaaggcatc gtgttcgggc atctcgacgc gagtctcggt gccgtcgtcg gagatgagac
     1321 acaactcgac gcgctcggcg acctcggaga acagcgcgaa gttgaccccc gaaccgccgt
     1381 aggtcgcccc cagggggtag ggctttccgg gccagatctg catcagtgcc ttcacaagtc
     1441 tctaggtggg gacgagaggg tatgccgtcc gtccggctcc cggcctcccc gccggacacc
     1501 cccatcctgc cagcacaccc accttccccg ctgcggcccg gccccaaccc cctccgagcg
     1561 ggccgcgctg gcggcttcgc gcgcggtcaa cgtcaaggcg accggatgcg tctatgagac
     1621 gtgggcacat tgcgaaacat cctgatattg actgcgattg ccgcgctgtc agcctgcgcg
     1681 ggacccgcga cgtcaccgcc gtcgagcgcc tcaacctcac cctcgtccct cggtggtttc
     1741 gaaaactcgc cagcctcaac gacgccgctc ctcgaccctg cacagctcgc cgtcgaggcg
     1801 ttcctgcgca cggaccccga ggtcgtcagg cggttcggcg ccaccccaca cgatggccgc
     1861 ctggcctgtg ccttcgcgcc gatcgttccc cgccgggaca atcacctctt tgcgcggttc
     1921 ctctgcctgg aggtcatggg gacagggcca ggggcgtaca gcatctccgg gagcgcgtcg
     1981 tcgatcgcca tgatcatcga cccagcgtcc ggcgccgtgc gcgattggcg cacgccgcgg
     2041 gacggcgccg acggcaggac agatctcttg gagatgttcc ccgctgacgt catgaacgcc
     2101 ttccgcagcg ccgacacggc aaccgccaac cggcagacct ccgagatgca gagccgatgc
     2161 ctcgccatcg cgaatgaact cgccgcagcg tcctgacggc gacgcnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnncgcga gtgcgtgacg gagtgcgatc gagggggtga
     2341 tcaccccgtc aggcgcactc cgtcacgcac tcactcgggt ggaaccagcc aaacgagccg
     2401 aaagctcagg cgagatgggc cacgacatcg tcggcggcgg catacgggtc ggtcgtgccg
     2461 gccaccacag cggcggcgtg ttcggcgatg ccgtgcccgg cccgcaggtc acccatccgg
     2521 gcccgcagcg ccgccagcgc gatcgcctcg atctcatcgg acgcccgccg caaccgacgg
     2581 gtatgccgcg tgcccgtcgc atcgagccag gcctcgtgct tgtcgatggc ctccatgacc
     2641 tcgtccagcc cctcgccgcg cgaggcgacg gtcttgcaca ccggcggccg ccacaggccc
     2701 ggctcggtgc ggtcgccgag agagatcatg tgccgcaggt cgcggaccgt cgcgtcggcc
     2761 ccgtcgcggt cggccttgtt gaccacgaac acgtcgccga tctcgaggat ccccgccttg
     2821 gcggcctgga tgccgtcgcc catccctggg gcgagcagga cgatcgtcgt gtcggccaga
     2881 ccggcgatct cgacctcgga ctgcccgacg ccgacggtct ccacgaggac gacctcgcag
     2941 ccggccgcgt cgagcacccg gatcgcttgc ggcgtggtcc acgccagccc accaaggtgc
     3001 ccgcgagagg ccatcgagcg gatatagacc tcggggtcga gcgcgtgctc ctgcatccgc
     3061 acccggtcac cgagcagcgc gccaccggag aacggcgagg acgggtcgac cgcgaggatc
     3121 cccacccgct tgccgcgggc ccggaacgcc gccaccaacg cgctcgtcga ggtcgacttg
     3181 ccgaccccgg gcgagccggt gagaccgatg acgtgagcct tgcccgtgtg aggggccagc
     3241 gccgccatca cctcacgcag tgcgggatgg gcatcctcga ccaacgagat caggcgcgcg
     3301 acggcgcggg gtgacccggc tcgggcagcg gtgaccagtg cggggacatc gaccgggcgg
     3361 ggcggcatac cgacctctcg ggagcggggt gaaggacacg gcgaccgaca gggtatgccg
     3421 cgcggtgccc gcagggggac gggcagccgc gcggcataca cgatgacaac ccgggaggac
     3481 gccgtgcggt tcccccgggg gaagatcagg ccttcgggac ccgcacgatg agcgcgtcgc
     3541 cctggccgcc gccgccgcag agggcagccg caccgacgcc gccgccgcga cgcttgagct
     3601 cgttggccag gtgcaagacg atccgggcgc ccgacatgcc gaccgggtgg cccatggcga
     3661 tggcgccgcc gttgacgttg accttgtcgg ggtcgacgcc gagctccttg gtggaggcca
     3721 gaccaacggc tgcgaaggcc tcgttgagct cgagcaggtc gaggtctgcg ggcgtgatgc
     3781 cctccttggc gcacgccttg acgatcgcgt gggcgggctg ggactgcagc gtcgagtcgg
     3841 ggccagcgac atagccggca gcgccgatct cggccagcca ggtcaacccg agagcctcgg
     3901 ccttcgcctt gctcatgacg acgaccgcag cggccccgtc ggagatctgc gaggcgctgc
     3961 ccgcggtgat cgtgccgtcc ttgcggaagg ccggccgcag cccggcaagg ctctcggccg
     4021 tcgtcgcggc gcgcacgccc tcgtcggcct tgaactcgat cgggtcgccc ttgcgctgcg
     4081 ggatcatcac cgggacgacc tcgtcgtcga acaggccgtc cttccatgcc ttggcggcca
     4141 actcgtggct gcgcgcggag aaggcgtcct gctcctcgcg ggtgaactcg cggtcaccgc
     4201 tgttggccga ctcggtgagg ttgcccatgc cctggtcggt gtagacgtcc cacagggcgt
     4261 cgtagtccat gtggtcgcgc atcttgac
//
LOCUS       DAORPW010000199         4266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6357_length_4266_cov_764.015673, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000199 DAORPW010000000
VERSION     DAORPW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6357_length_4266_cov_764.015673"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLB86_11685"
     CDS             complement(<1..491)
                     /locus_tag="PLB86_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRC65846.1"
                     /translation="MSTRVWWLRRDLRVADHPALLAAADGGAVVPLFVLDPALWAAAG
                     APRQARLLASLRSLDADLRDRYAVSLVVRVGSPLTVVPALATEVGAAAVHVTAETTPY
                     GRRRDTAVRAALNAQGRELVATGTAYAIGPGTVRTGGGTPYRVFTPFARAWRALGAPG
                     PAPA"
     gene            511..1257
                     /locus_tag="PLB86_11690"
     CDS             511..1257
                     /locus_tag="PLB86_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRC65847.1"
                     /translation="MTDMIDTTEMYLRTIFELEEDGIPPLRARIAERLGHSGPTVSQT
                     VARMERDGLLAVTDHRHLELSESGRRAATRVMRKHRLAERLLVDVLGLEWELAHAEAC
                     RWEHVISDEVERRILALVGDSTRSPYGNAIPGLAELGGQVGRDTTAERDSEEPLTEVL
                     GVSDHGPIAGAKASGESVVVLTSRIGEPVQVDHTALSLLTVAGLVPGRSARVSLQGNE
                     IRAIPEGVADREPVVVPTEVAAYVFVFRRA"
     regulatory      1445..1621
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1624..2478
                     /locus_tag="PLB86_11695"
     CDS             1624..2478
                     /locus_tag="PLB86_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRC65848.1"
                     /translation="MAKHVGRHRAPGFSPLTELTQIAGDSAKPAVRATAVMAVSTGML
                     AGMVVPAQAATIVAGVDGVSSDGQALDTPVSDLLGISGLPSTDGPGLAPAAPAPVAAT
                     GLVEAVEAAVGPVVTAPDTVAGLPTFGALGFKTKTITVPAVTQVSRSTAPRTSPAPAA
                     KAATPAPAPAPVSGGSVLSVAASLLGIPYRYGGSSPSTGFDCSGFTSYVYRIARGITI
                     PRTAEGQRKGSARVTSPVPGDLVFFGVPATHVGIYAGNGMMYDSPHTGTVSQLRKIYS
                     GNVSYGRF"
     gene            complement(2556..2825)
                     /locus_tag="PLB86_11700"
     CDS             complement(2556..2825)
                     /locus_tag="PLB86_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65849.1"
                     /translation="MSTSTTTTPTTTTERSGFWASVRDDLRERREARAIRAQMFRELE
                     GYQSRGDILDLLAAADRHEGPEAELMRGVLQSKLADVNRSSRRAS"
     gene            2904..3848
                     /locus_tag="PLB86_11705"
     CDS             2904..3848
                     /locus_tag="PLB86_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRC65850.1"
                     /translation="MDTEVLRWFQQVADGATVTEVADLWMVSQPKVSRDLMALQREVG
                     APLLVKSGRVLRLTHAGAVFKRHADAIVNRLDDALAAVDQLVDPERGTVTLAYERTLG
                     TWLVPSLVGEFLAVHPQVSFTLVVGGEDLAVLDEGRVDLAVLASRPNAPLFGGAREGG
                     GASEASVRWEPLFREPLFLAVGPGHRFEDIETVPLAELADEPWVVLPPGMVIRSALDQ
                     LCHGAGFEPKVAFECDDLATVRGFVGAGLGVSIVPAMGEDPLPRYAAAPRLVALADAG
                     ARREVGLAWSSQRRLLPAAELFRTFVLRSSGAGRTIAS"
     gene            3877..4212
                     /locus_tag="PLB86_11710"
     CDS             3877..4212
                     /locus_tag="PLB86_11710"
                     /inference="COORDINATES: protein motif:HMM:NF026933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm51 family immunity protein"
                     /protein_id="HRC65851.1"
                     /translation="MDPLNLIETAPDDVALTMYTGELVVDAMVRKLGHEPNGTFWDGV
                     AEWLVETEAPTLEGRFDYDSEGGMFCAYGDRADLEALRALMLPVVTDPKRVRSLIKAA
                     EDADFEFDD"
BASE COUNT          607 a         1472 c         1498 g          689 t
ORIGIN      
        1 gcgggcgccg gcccaggcgc ccccagcgct cgccaagcgc gcgcgaacgg cgtgaacacc
       61 cgatagggcg tcccacctcc cgtccgcact gtcccaggcc cgatcgcgta tgccgtgccc
      121 gtcgccacga gctctcgacc ctgggcgttc agagcggccc ggacagcggt gtctcggcgg
      181 cgtccatagg gcgtggtctc ggccgtgaca tgcacggcag ccgcaccgac ctcagtcgcc
      241 agggccggga ccacagtcaa aggtgagccg acccggacga cgagcgacac ggcatacctg
      301 tcgcggaggt cggcgtcgag cgagcggaga gacgcgagca agcgggcctg ccgcggcgca
      361 ccagcggccg cccagagggc tgggtcgagg acgaagagcg gaaccacggc cccgccgtcg
      421 gccgcggcga gcagggcagg gtggtcagcg acgcgcaggt cgcgacgcag ccaccacacg
      481 cgtgtgctca tcggtgagag gctagccccc gtgaccgaca tgatcgacac cacggagatg
      541 tatctgcgga ccatcttcga gctcgaggaa gatgggatcc cgccgctgcg cgctcgcatc
      601 gccgagcgcc tggggcattc tgggccgacg gtgtcgcaga cggtcgcgcg aatggagcgc
      661 gacggcctcc tcgccgtgac cgaccaccgc cacctcgagc tgtccgagtc gggtcgccgg
      721 gccgccacgc gggtgatgcg caagcaccgc ctcgccgagc gactcctcgt cgacgtgctc
      781 ggattggagt gggagttggc gcacgccgag gcctgccggt gggagcacgt catcagcgat
      841 gaggtcgagc ggcggatcct ggctctcgtc ggagattcca cgcgctcgcc ctatggcaac
      901 gccatccctg ggctggccga gctcggcggg caggtgggcc gcgacacgac ggcggaacga
      961 gacagtgagg aacccctcac tgaggtgctc ggcgtcagtg atcacggccc catcgcgggg
     1021 gccaaggcgt ccggcgagag cgtcgtcgtg cttaccagcc ggatcgggga gccggtccag
     1081 gtggatcaca ccgcgttgtc gctgctcacg gtggctggcc tcgtgcctgg tcggagcgcg
     1141 cgagtctcct tgcagggcaa tgagattcgc gcgatccctg agggtgtcgc cgatcgcgaa
     1201 cccgttgtag tgccgaccga ggtcgccgcc tacgtgttcg tctttcggcg cgcctgagcg
     1261 ggcggtcacg acgatccggt atgccgccgc gcggcatacc tgctgatcgc cagccgaggc
     1321 ggtcgtgacc ttgtggacgc cattccgtga ccttggcgtg acattcgggg gtcccgtcgg
     1381 ctaaagtttg ggcactcgcc cagcaccggg cgggccccga agcggatcgc cgagtcctgc
     1441 caccgccgag ggtttgctca aaccgccagg caggagcggg ggacccactt agtgggcgtc
     1501 gtgagacgcc cttggggtga agttcgcgga tcggcccctc accgggccgg gccgagagcc
     1561 gggtgatcaa ctcccacccg aacccgacag ctcacctcgt aggcgttctt ggagaggtac
     1621 atcgtggcaa agcacgttgg gcgccaccgc gccccggggt tctcccccct gaccgagctg
     1681 acccagatcg ctggcgattc cgccaagcct gccgttcgcg ccactgccgt catggctgtg
     1741 tccaccggca tgctcgcggg catggtcgtg ccggcacagg cggccaccat cgtggccggc
     1801 gtggatggcg tgtcttcgga cggtcaggca ctcgacactc ccgtcagcga ccttcttggc
     1861 atcagcggcc tgccgtcgac cgatggcccg ggcctggcgc ctgcggcccc ggccccggtt
     1921 gcggccaccg gtctggttga ggccgtcgag gctgcggtcg gccccgtcgt gaccgctccc
     1981 gacaccgttg cgggcctgcc caccttcggt gcgttggggt tcaagaccaa gacgatcacc
     2041 gtccccgcgg tcacccaggt gtcgcgctcg accgcgccgc gcacctcgcc ggctccggcc
     2101 gccaaggctg cgacgcccgc cccggccccg gcgcctgtgt ccggtgggtc ggtcctctcg
     2161 gttgcggcca gcctgctcgg catcccctac cgttacggcg gttcttcgcc gtcgaccggt
     2221 ttcgactgct ctggtttcac ctcttacgtc tatcggatcg cccgtggcat cacgatcccg
     2281 cgcacggccg agggccagcg caagggctcg gcgcgcgtca cctccccggt gcctggcgac
     2341 ctcgtcttct tcggcgtccc ggccacacac gtgggcatct acgccggcaa cggcatgatg
     2401 tacgactcgc cgcacaccgg cacggtcagc cagctgcgca agatctactc cggcaacgtc
     2461 tcgtacggcc ggttctgatc gaggcgaact aggcccagag acacaacgaa aggccccgcc
     2521 cgggttgccg ggcggggcct ttcgctctgg ttgggtcagc tggcccgacg cgacgagcgg
     2581 ttgacgtcgg ccaacttgct ctgcagcacg ccgcgcatca gctcggcttc ggggccctcg
     2641 tgccggtcgg cggcggcgag gaggtcgagg atgtcgccgc ggctctggta gccctcaagc
     2701 tcgcggaaca tctgggcacg gatcgcgcgg gcctcgcggc gctcgcggag gtcatcgcgg
     2761 acgctggccc agaacccgct gcgctcggtg gtggtggtcg gggtggtggt cgttgaagtg
     2821 ctcatggcct caatggtgcc ccgaccagat tcatacgtcc aatgtgatga tgctattgaa
     2881 tccatgcgct agcgtcatga gtcatggata ctgaggtgct gcgctggttc cagcaggtcg
     2941 ccgacggggc aaccgtcacg gaggtggccg atctctggat ggtcagtcag cccaaggtct
     3001 cgcgcgacct catggccctg cagcgtgagg tgggggcgcc gttgctcgtc aagtccgggc
     3061 gggtgctgcg cctcacccac gccggtgccg tcttcaagcg ccacgccgac gccatcgtca
     3121 accgcttgga cgacgccctc gcggccgttg accaactcgt cgacccggag cgaggcacgg
     3181 tcaccctcgc ctacgagcgg accctgggca cctggttggt gccgtcattg gtcggggagt
     3241 tcctcgcggt gcatccccag gtgtcgttca cgctcgttgt cggcggtgag gatctggccg
     3301 tgctcgatga gggccgggtc gatctcgctg tgctcgccag ccgacccaac gcaccgctgt
     3361 tcggcggggc gagagagggg ggtggggcga gcgaggcgag cgtgcgctgg gagccgttgt
     3421 ttcgcgagcc attgttcctc gcggtcggcc ccggccaccg gttcgaggac atcgagacgg
     3481 tgccgctggc cgagttggcc gacgagccgt gggtggtcct gccgccgggc atggtgatcc
     3541 gctcggccct cgaccagttg tgccatggag caggcttcga gccgaaggtc gccttcgaat
     3601 gcgacgacct cgccacggtg cgcggcttcg tcggagcggg cctgggcgtc tcgatcgtcc
     3661 ccgcaatggg ggaggacccc ctgcctcggt atgccgccgc gcctcgcctt gtcgcgctcg
     3721 ccgacgccgg ggcccgtcgc gaagtcggac tcgcctggtc gtcccagcgg cggttgctcc
     3781 cggcggccga gttgttccga acctttgtcc tgcgttcgtc gggggcgggt cggaccatcg
     3841 catcatgagg ccgggcgggt ctgcaacgat gctccgatgg atcccctaaa cctcatcgag
     3901 accgccccgg atgatgtcgc cttgacgatg tataccggcg agttggtcgt cgatgccatg
     3961 gtgcgcaaac tcggtcacga gccgaatggc accttctggg acggcgtggc cgaatggctc
     4021 gtcgagaccg aggcgcccac gctggaaggg cgattcgact acgacagtga aggcgggatg
     4081 ttctgcgcct acggcgaccg cgccgacctc gaagcgctgc gggccctgat gctccccgtc
     4141 gtcacggacc ccaagcgggt ccggtcactc atcaaggccg ccgaggacgc cgacttcgag
     4201 ttcgacgact gacggcgcct cggcggaaac tacggcaacg gcggcagcgg caacggcggc
     4261 aacggc
//
LOCUS       DAORPW010000200         4258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6375_length_4258_cov_616.004759, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000200 DAORPW010000000
VERSION     DAORPW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6375_length_4258_cov_616.004759"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PLB86_11715"
     CDS             complement(<1..743)
                     /locus_tag="PLB86_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRC65852.1"
                     /translation="MSEMLSIDFMQRALLAALLVGLSAPLVGVFLVQRRLALIGDGMG
                     HVALAGVAVGVWLGQSPVWTALIGAVLAAIIVELIRTSGSAHGDVALAIIFYGGIAAS
                     VVIIGKSPNGTPASLTAYLFGAITTVTAADLWVFGVLTVVILLVVGVLRWRLFAVASD
                     EEYARSAGLPVLALNLALAVLTAVTVVVSMRIVGLLLISALMIVPNAAAQLLGHSFRS
                     SLAWAVAIGVACAVGGVVVSYAADTPSGGT"
     gene            complement(740..1585)
                     /locus_tag="PLB86_11720"
     CDS             complement(740..1585)
                     /locus_tag="PLB86_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRC65853.1"
                     /translation="MSTPTAPPTPDPPATAPPVIALRGASFGYGDHAVVHDVDLTITA
                     GEVVLVLGPNGSGKSTIAKGLFGLTDLLAGEVEVFGMPRADFRDFPRLGYVPQRHTLA
                     SSVTVTVSEVVSSGRLSRQSWLGIATRADRQQVRRALELVGLADRAGIDVGALSGGQQ
                     RRVLIARALAGEPDVLVMDEPTAGVDIASQHGLVEVLARLVAEGLTLVVVTHELAAFA
                     DLVTRVVVVDAGRIAFDGSRDEFVAAGSDVLHDHDWHHHLDRPDRRPAVISAEGPLSQ
                     GGSRE"
     gene            complement(1582..2547)
                     /locus_tag="PLB86_11725"
     CDS             complement(1582..2547)
                     /locus_tag="PLB86_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRC65854.1"
                     /translation="MPRTAPARPRPTSRANRTRVIRAAAWGAVAVLALVSACASPRAA
                     QESAEAATAQVPVSAAFYPLAYLLEQVGGPGVRVWTITKPGVEPHDLELTPKDLVDLP
                     KMSLVAYLKGFQPAVDEAVAQQAPSAAYDVSAAADLTLGAPAGEASATDLHFWLDPIR
                     YAAVGTALGERLALADPTHAADYRARAKTFAASMEQLDKTFATGLASCATKQLVTSHA
                     AFGYLAARYGLTQVPIAGLSPDQEPSAKQLADVAATVKAAGVKTIFSETLVDPKFAQT
                     IAQETGATLALLDPVEGITDQSAAKDYPGVMLANLAALRTGLGCS"
     gene            2688..3587
                     /locus_tag="PLB86_11730"
     CDS             2688..3587
                     /locus_tag="PLB86_11730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC65855.1"
                     /translation="MSALEWRPLTSDDLGQIVALAQECLEADGGLPQLGTDETIQALF
                     LQGEGVTGCDETGEIVAAAAMSRDSAGRVVATGLVHPSMRRQGHGEALVDWCRAHADG
                     QPIRVVAETMSIEAESLFAANNLHRTFAEAVMRHKLKHISRVALPEGLHTEPFRDDTA
                     HAFYAAYRSSFGDRPGFPDPPEEEWLRWLVDDSGFRPEDSRVALTEDGEPAGFITVSD
                     EWIDQVGVVPEWRGQGLGAHLVVRSLTALKKAGASRVWLCVGVDNGARALYERLGFAH
                     KGTRARYEERGAVRRAAREAGLV"
     gene            complement(3606..3893)
                     /locus_tag="PLB86_11735"
     CDS             complement(3606..3893)
                     /locus_tag="PLB86_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65856.1"
                     /translation="MSLSFRSKVEHASVQWVTRLNRVPRWAAFIGVLALMVAGILVPK
                     VGFLFTLVIAAFLGWLIFLTWPRLAAPEKLMRIAVLALVVAVAVMQAFPKA"
     gene            4027..>4258
                     /locus_tag="PLB86_11740"
     CDS             4027..>4258
                     /locus_tag="PLB86_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRC65857.1"
                     /translation="MASSTSTVDTVVSLCKRRGFVFPCGEIYGGTRSAWDYGPLGVEL
                     KENIKRQWWKAMVHAREDVVGLDSSIILPRQVW"
BASE COUNT          696 a         1342 c         1637 g          583 t
ORIGIN      
        1 gtgccgcccg agggggtgtc ggcggcatac gacacaacga ccccgccgac cgcgcaggcg
       61 acgccgatcg ccacggccca cgcgagcgac gaccggaagc tgtggccgag gagttgggcg
      121 gccgcgttgg ggacgatcat caaggcgctg atgagtagca agccgacgat ccgcatcgac
      181 acgacgacgg tgactgcggt gaggaccgcc aaggcgaggt tgagggcgag gacgggcagg
      241 ccggccgatc gggcgtactc ctcgtcgctg gcgaccgcga acagccgcca ccgcaggacc
      301 ccgacgacga ggaggatgac gaccgtcagc acgccgaaga cccacaggtc agcggccgtc
      361 accgtcgtga tcgctccgaa gaggtatgcc gtgaggctgg ctggggtgcc gttgggggac
      421 ttcccgatga tgacgacgct ggcggcgatg ccgccataga agatgatcgc caacgcgaca
      481 tcaccgtgcg cgctgccgga tgtgcggatg agctcgacga tgatcgcggc cagcaccgcg
      541 ccgatgagcg cggtccacac gggtgactga ccgagccaca cgccgacggc caccccggcg
      601 agggcgacat gccccatccc gtcgccgatg agggccaggc gccgttggac gaggaagacg
      661 ccgacgaggg gagcggacaa ccccaccagc agggcagcca gcagggcccg ttgcatgaag
      721 tcgatggaca gcatctcgct cattcgcgcg aacctccctg ggagagaggg ccttccgcgg
      781 agatcaccgc aggccgacgg tcggggcggt cgaggtggtg atgccagtcg tggtcgtgca
      841 ggacgtccga tccggccgca acgaactcgt cccggctgcc gtcgaacgcg atccgcccag
      901 catcgacaac gacgacccgg gtcaccaggt cggcgaaggc ggccaactcg tgggtgacga
      961 cgacgagggt gaggccctcg gcgacgagcc gcgcgagcac ctcgaccaac ccgtgctggc
     1021 tggcgatgtc gacgccggcc gtcggttcgt ccatgacgag gacgtcgggc tcgccggcca
     1081 atgcgcgggc aatgagcacg cggcgctgtt ggcccccgga cagggcgccg acatcgatgc
     1141 cggcccggtc ggcgaggccg acgagttcga gcgcccggcg gacctgctgc cggtcggcgc
     1201 gggtggcgat gcccaaccag gactgccggg acagtcggcc cgaggacacg acctccgaga
     1261 ccgtgacggt cactgaggag gcgagggtat gccgctgggg cacgtacccg aggcgcggga
     1321 agtcgcggaa gtccgcgcgc ggcataccga agacctccac ctctccggcg agaaggtcag
     1381 tgagcccgaa cagccctttg gcgattgtcg atttcccgga gccgttgggc cctaggacga
     1441 ggacgacctc accggcagtg atggtgaggt cgacgtcgtg gacaacggcg tgatcgccgt
     1501 agccgaagga ggctccgcgc agcgcgatga ccgggggagc cgtggcgggt ggatcgggcg
     1561 tggggggagc ggtgggcgtg ctcatgagca gcccagtccg gtgcgcaggg ccgcgaggtt
     1621 ggcgagcatg acgccggggt agtccttcgc ggccgactgg tcggtgatcc cctcaaccgg
     1681 gtcgagcagg gccagcgtgg cgcccgtctc ctgggcgatg gtctgggcga acttcgggtc
     1741 gacgagggtc tcggagaaga tcgtcttgac cccggccgcc ttgaccgtcg cagcgacgtc
     1801 ggccaactgt ttggcggacg gttcctggtc gggggacagg ccggcgatcg ggacctgggt
     1861 gaggccgtag cgcgcggcga ggtagccgaa agcggcatgg ctggtgacga gttgcttggt
     1921 ggcacacgag gccaggccgg tcgcgaaggt cttgtccaac tgctccatcg aggccgcgaa
     1981 cgtcttggcg cgggctcggt agtcggcggc atgggtgggg tcggcgaggg cgaggcgttc
     2041 cccgagggcg gtgccgacgg cggcataccg gatggggtcg agccagaagt gcaggtcggt
     2101 cgcgctcgcc tcgcccgcgg gtgcgccgag cgtcaggtcg gctgcggcgg aaacgtcgta
     2161 tgccgcgctt ggcgcctgct gcgcgaccgc ctcgtcgacg gcgggctgga agcccttgag
     2221 gtaggcgacg agcgacatct tgggcaggtc gacgaggtcc ttcggggtga gttcgagatc
     2281 gtgcggctcg acccccggct tggtgatggt ccagacgcgc acccctggac cgccgacctg
     2341 ctcgaggagg taggcgagcg gatagaacgc cgcggagacg gggacctgtg ccgtggccgc
     2401 ctcggcgctc tcctgggccg cccgggggga cgcgcacgcg ctcaccaacg ccaggacagc
     2461 gaccgcgccc caagcggccg cgcgaatgac acgtgtccgg ttggctcggc tggtcgggcg
     2521 ggggcgggcg ggagcggtgc gcggcatacc tccacggtcg cggaaatgag aatcattgtc
     2581 aacttggggt cggtgatgac gcgctagagg gccctgtgct ctcatggtgg gagcgcggtg
     2641 ggcccgaccc gccgcagccg gactggaaca gcgggaagag gaagcctgtg tcagcactgg
     2701 agtggcgccc gctcacgagc gacgatctgg gccagatcgt tgccctggcc caggagtgtc
     2761 tggaggccga cggcgggttg ccgcaactgg gcaccgacga gaccatccag gcgctgttcc
     2821 tgcagggtga aggcgtcacc ggctgcgacg agaccggcga gatcgtcgcc gcggctgcga
     2881 tgagtcgcga ctcggccgga cgcgtcgtcg ccaccggcct ggtgcacccg tcgatgcggc
     2941 gccaaggtca tggggaggcg ttggtcgact ggtgtcgtgc gcacgccgac ggccagccga
     3001 tccgggtcgt ggccgagacg atgtcgatcg aggccgagtc gttgttcgcc gccaacaacc
     3061 tgcaccgcac cttcgccgag gcggtcatgc ggcacaaact caagcacatc tcccgagtcg
     3121 cgctgcccga aggcctgcac accgagccgt tccgggacga cacggcgcac gcgttctacg
     3181 cggcataccg gagctccttc ggcgaccgac cggggttccc ggacccgccc gaggaggagt
     3241 ggctgcgctg gttggtcgac gactcgggtt tccgccccga ggactcgcgg gtcgcgttga
     3301 ccgaggacgg cgagccggcg ggcttcatca cggtgtcgga cgagtggatc gaccaggtgg
     3361 gggtcgtgcc ggaatggcgc ggccagggtt tgggggcgca cctcgtcgtc cgctcgctga
     3421 cggcgctcaa gaaggccggg gcgagccggg tgtggctgtg tgtcggcgtc gacaacgggg
     3481 cgcgggcgct ctatgagcgg ctgggtttcg cccacaaggg gacccgggcg cggtacgagg
     3541 agcgtggggc cgtgcgtcga gccgcccgcg aggccgggct ggtctgacgg gtttctgacc
     3601 gggtttcagg ccttggggaa ggcctgcatg acggcgacgg cgacgaccaa agccaggacc
     3661 gcgatgcgca tgagcttctc gggggcggcc aggcgcggcc aggtgaggaa gatcaaccag
     3721 ccgaggaagg ccgcgatgac cagcgtgaag aggaacccga ccttgggcac gaggatcccg
     3781 gcgaccatga gggcgaggac gccgatgaag gctgcccacc gtggaacccg gttgagccgc
     3841 gtcacccact ggacggaggc gtgctcaacc ttggaccgga acgaaagact cacggcggac
     3901 agcctaaggg agcgtctgcg cggtgctgaa cgccggtgcc gaacgccggt gccgaaggct
     3961 ggcggcctac acccggcgcc gaaacccagt cagggggtat gccgctgggc cgataacctg
     4021 tcctgcatgg cttcgagcac ctccaccgtc gacaccgtcg tcagcctctg caagcgcagg
     4081 ggcttcgttt tcccgtgcgg ggagatctac ggagggacgc gctcggcctg ggactacggg
     4141 ccgttgggtg tcgagctcaa ggagaacatc aagcggcagt ggtggaaggc gatggtccac
     4201 gctcgcgagg acgtcgtcgg cctggactcc tcgatcatcc tgccccgcca ggtgtggg
//
LOCUS       DAORPW010000201         4255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6385_length_4255_cov_678.196429, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000201 DAORPW010000000
VERSION     DAORPW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6385_length_4255_cov_678.196429"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLB86_11745"
     CDS             <1..711
                     /locus_tag="PLB86_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65858.1"
                     /translation="DPGRRQGQQAEAPVRRGGIPAGGEPEPQVGALFAVTYDSRRWIP
                     APPSRRIPSDGADHFDRWVTLAGSRFLTPADDPAGPKAAAIGRILRTLGSPDTPSWDP
                     FWLQKWIHWPSLADLPTMVIVSGFPATGGGTTDELRGYCGEGVPGVLEEPIIEPVSST
                     RLGSGLRSLAYTAEMADGLAEVHATLAYVWRIRLLDDGRPTLGIVRLWASGSPRQIIA
                     AEPDIHDLALTLRMETSG"
     gene            704..>1341
                     /locus_tag="PLB86_11750"
     CDS             704..>1341
                     /locus_tag="PLB86_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65859.1"
                     /translation="MADDDLPETDDDAPVLRAVPDSAVGYRLVLPPGWLRLDARADAG
                     RARLDAELDRVLPKTYQPTLAPVVAQARAQAHSALHQARDAGAIDLYLPLGGVRGIPI
                     PASFLVAVRRFAAAPPPTLAAQGPEALAVALIANLAATMPDAKTRMMAAGPAVRSTRP
                     VTGAASDTSPALTGVRVDYIWPVPGDAALWVMASFTTMRTPELPASITDQLVA"
     assembly_gap    1342..1441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1522..3051)
                     /locus_tag="PLB86_11755"
     CDS             complement(1522..3051)
                     /locus_tag="PLB86_11755"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRC65860.1"
                     /translation="MPRNKFRTVAEAVALISDGATVGLVGGGSGLQEAGALFAEVERR
                     FLETGHPAGLTLVHALGIGDKAERGLNRFAHRGLVSAVIGGHWVWSPRMQALAADNEI
                     EAYVLPGGVVGQLYREIGAGRPGLFTHVGLGTFVDPRHGGGRMNAAATRTLADVLEID
                     GREVLRYRPFPVDIALLRGSYADADGNISFAQECANLDMFDLALAAHNSGGKVIVQVR
                     TAVDRGTIPARDVHIPGAWVDAVVVDPTQSMTYDIVYDPTMSGEVRGPVPPRPPEPFT
                     ERVAVARRAALELRDDAVVNYGFGMPDLVARIVADRGDTERYYQTIEHGVYGGDLLTG
                     SLFGFGRNATAYIHGPSQFDFYAGGGLDLAFLGFGEFDAQGNVNVSMLGGVTVGPGGF
                     MDIAANARKVVFCGTFEAKGLRVRPVDGRLVVDQPGSVPKVVAQVDQVTFSGPQAVAR
                     GQEVLYVTERAVFRLTPEGIVLAEVAPGIDVQTQVLDRMGFAPLLPQPPAVIDGAIFR
                     E"
     gene            complement(3068..>4255)
                     /locus_tag="PLB86_11760"
     CDS             complement(3068..>4255)
                     /locus_tag="PLB86_11760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC65861.1"
                     /translation="PRTAAMDFTLTDEQRMLVKTIRRFVYDELIPLEDDIEATGALDP
                     AAARSIFEKSRDLGFYAMNIPEEFGGGGLSALETMLVEEQFGHCKDILIRRAFGNVYE
                     VLLEGDAAQRERWLLPTVSGERTCSIAITEPGAGSDAASITTRAERTATGWTLNGGKY
                     FVSDGLYSDFFIVSAVTDAAARPRHISLFLVDKNLPGVRVGQDQKMMGLAGSSHIELF
                     FDDVHLGPETLIGGEGNGLRLALTALGRVRLAQVGARAVGKASRLLGYMADYAGQREQ
                     FGSPIGSFQMVQAMLADSAIEINRSRLLLHRAAAALDAGEDYRSWISMVKVDAAEMLG
                     RVADRAVQVFGGTGYTHGFPVERMYRDARIFRIFDGTSEIHRTVVAAGVLKGGAKTWD
                     IGA"
BASE COUNT          644 a         1425 c         1488 g          598 t
ORIGIN      
        1 gatcctggcc gcagacaggg ccaacaggcg gaagctccgg tgaggcgggg cggcatacct
       61 gcgggtgggg agcccgagcc ccaggtcggc gcgctcttcg ccgtcactta cgactctcgg
      121 cgctggatcc cggccccgcc gagtcgtcgg atccccagcg acggcgcgga ccacttcgac
      181 cgctgggtca cgctggccgg cagccggttc ctcactcccg cagacgaccc ggcgggcccc
      241 aaggcggcgg cgatcgggcg gatcctgcgc accctcggca gccccgacac tccttcatgg
      301 gacccgttct ggttacagaa gtggatccac tggccgagcc tggcggacct gccgacgatg
      361 gtcatcgtca gtgggttccc ggcgaccgga ggtgggacga ccgatgagct gcggggctac
      421 tgcggcgagg gcgtgcccgg cgtgttggag gagccgatca tcgagccggt gtcctcgaca
      481 agactcgggt ccgggctgcg cagcctggcc tacacggcgg agatggccga tgggctggcc
      541 gaggtccacg cgacgctggc ctacgtctgg cggatccggc tgctcgacga cgggcggccg
      601 acgctaggca tcgtgcggct gtgggccagc gggtcgccgc gccagatcat cgcggctgag
      661 ccagacatcc atgaccttgc cctgaccctt cggatggaga ccagtggctg acgacgacct
      721 tccagagacc gacgacgacg cccccgtcct gcgggcggtg cccgacagcg cggtgggcta
      781 tcgcctcgtc ctgccgcccg gatggctgcg actggacgcc cgggccgacg ccgggcgcgc
      841 ccggctggac gccgagcttg accgggtcct gcccaagacc taccaaccga cgctcgcgcc
      901 ggtcgtggcc caggctcggg cgcaggccca ttcggcgctg caccaagccc gtgacgccgg
      961 ggccatcgac ctctacctgc cgttgggggg cgtgcgcggc ataccgatcc cggcgtcgtt
     1021 ccttgtcgcg gtccggcgct tcgcggcggc gcccccgccc accctggccg cccagggccc
     1081 ggaagcgctc gccgtcgcgc tcattgccaa cctggccgcc accatgcccg acgcgaagac
     1141 ccggatgatg gcggcggggc cggcggtgcg ctcgacccgg ccggtcactg gggccgcctc
     1201 cgacacttcg ccggccttga ccggggtgcg ggtcgactac atctggccgg tgccgggcga
     1261 tgccgcgctg tgggtgatgg cgtcgttcac gacgatgcgg acgccggagt tgccggcgag
     1321 catcaccgat cagttggtcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 ncccctctgc cgggtcgagg tattccgcca tcaccaggcg atggcggaat acctcgaccc
     1501 ggcaagagag gggggcgaga gtcactcgcg gaagatggct ccgtcgatga ccgcgggcgg
     1561 ttgtggcagc agcggagcga aacccatccg gtcgagcacc tgggtctgga cgtcgatccc
     1621 aggcgcgacc tcagccagca cgatgccctc aggggtgagc cggaagaccg cacgctcggt
     1681 cacatagagc acctcctgtc cgcgggccac cgcctgcggc cccgagaagg tcacctgatc
     1741 gacctgggcg accaccttgg gcaccgaccc gggctgatcg accacgagtc gaccgtcgac
     1801 cggccggacc cgcaaaccct tggcctcgaa ggtcccgcag aacaccacct tgcgcgcatt
     1861 cgccgcgatg tccatgaagc cgcccggccc gaccgtcact ccgccgagca tcgagacgtt
     1921 gacgttcccc tgcgcgtcga actcaccgaa gccgaggaag gccagatcca gcccgccgcc
     1981 ggcatagaag tcgaactggg acggaccatg gatgtatgcc gtggcgttgc gaccgaagcc
     2041 gaagagcgac ccggtgagca ggtcgccgcc atacacgcca tgctcgatgg tttggtagta
     2101 gcgctcggtg tcgcctcggt cggcgacgat gcgggcgacg aggtccggca taccgaagcc
     2161 gtagttgacc accgcgtcgt cgcgcagttc caaggcggcc cggcgggcga cggcgactcg
     2221 ctcggtgaac ggctcgggcg ggcgcggcgg cacggggccg cggacctcgc cggacatggt
     2281 cgggtcgtag acgatgtcat aggtcatcga ctgggtgggg tcgacgacga ccgcatccac
     2341 ccaggcgccg gggatgtgga cgtcgcgggc cgggatggtg ccgcggtcga ccgcggtgcg
     2401 gacctggacg atgaccttgc cgccgctgtt gtgggcagcc agggcgaggt cgaacatgtc
     2461 gaggttggcg cactcctggg cgaaggagat gttgccgtcg gcatcggcat acgacccgcg
     2521 cagcagggcg atgtcgacgg ggaacgggcg gtagcgcagg acctcgcggc cgtcgatctc
     2581 cagcacatcg gcgagcgtgc gcgtagccgc ggcgttcatc cggccaccgc catgccgggg
     2641 gtcgacgaag gtgcccagcc cgacgtgggt gaacaggcct ggccgcccgg cgccgatctc
     2701 gcgatagagc tggccgacga caccaccggg caggacgtag gcctcgatct cgttgtcggc
     2761 ggccagggcc tgcatcctcg gggaccacac ccagtggccc ccgataaccg ctgagaccag
     2821 gccgcgatgg gcgaaccggt tgaggccccg ctcagccttg tcgccgatgc cgagggcgtg
     2881 gacgagggtc agccccgcgg ggtgacccgt ctcgaggaac cgacgctcga cctcggcgaa
     2941 cagggcaccg gcctcctgca gaccgctgcc gccaccgacg agcccgacgg tcgcgccgtc
     3001 ggagatgagt gccacggcct cggcgaccgt gcggaacttg ttgcgcggca taccaactgg
     3061 cctttcccta tgccccgata tcccaggtct tcgcgccgcc tttgagcact ccggcggcga
     3121 cgaccgtgcg gtgaatctcg gaggtgccgt cgaagatgcg gaagatgcgg gcgtcgcggt
     3181 acatccgctc gacggggaag ccgtgggtgt agcccgtgcc gccgaagacc tgcacagctc
     3241 ggtcggcgac ccggccgagc atttcggccg cgtcgacctt gaccatggag atccacgagc
     3301 ggtagtcctc gcccgcgtcg agtgcggcag ccgcgcggtg cagcagcaac cgcgaccggt
     3361 tgatctcgat ggcgctgtcg gcgagcatcg cctggaccat ctggaaactc ccgatcggcg
     3421 acccgaactg ctcacgttgg ccggcgtagt cagccatgta accgagcagc cgcgacgcct
     3481 tgccgaccgc ccgcgcaccg acctgggcca gccggacgcg acccagtgcg gtgagggcca
     3541 ggcgcaggcc gttgccctcg ccgccgatga gggtctcggg cccgaggtgg acgtcgtcga
     3601 agaacaactc gatgtgggag gacccggcca ggcccatcat cttctggtcc tgaccaaccc
     3661 ggacgccggg caggttcttg tcgacgagga agagcgagat gtggcgcggg cgggcggcgg
     3721 cgtccgtgac cgccgagacg atgaagaagt cggagtagag gccgtcgctg acgaagtact
     3781 tgccgccgtt gagggtccag ccggtcgccg tgcgttcggc gcgggttgtg atggaggcgg
     3841 cgtcggagcc cgcccccggc tcggtgatgg cgatcgaaca ggtgcgctcg ccgctcacgg
     3901 tgggaagcag ccaacgctca cgctgcgcgg cgtcgccttc gaggagcacc tcgtagacgt
     3961 tgccgaaggc ccgccggatg aggatgtcct tgcagtggcc gaactgctct tcgacgagca
     4021 tggtctcaag ggctgacagc ccgccgccgc cgaactcctc aggaatgttc atcgcgtaga
     4081 agccgagatc acgggacttc tcgaagatcg accgggccgc ggcggggtcg agggcgccgg
     4141 tcgcctcgat gtcgtcctcg agcgggatca gctcgtcgta aacgaatcga cggatcgtct
     4201 tgacgagcat ccgctgctcg tcggtgagcg tgaagtccat ggctgccgtc cgggg
//
LOCUS       DAORPW010000202         4246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6409_length_4246_cov_779.642567, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000202 DAORPW010000000
VERSION     DAORPW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6409_length_4246_cov_779.642567"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PLB86_11765"
     CDS             <1..671
                     /locus_tag="PLB86_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC65862.1"
                     /translation="QTLGEASHRLSFWLLAGTFFICGWSTNGLIGTHFIPAAHDHGMP
                     PTTAAGLLALIGIFDIAGTVASGWLTDRVDPRLLLVGYYVLRGLSLLVVPSVLGPGVE
                     PSLFLFIVFYGLDWVATVPPTMALCRAHFGLERSSIVFGWVFAAHMVGAGIAATFAGW
                     VRQSSGDYFWAWLTAGILCLIAGFACLAIPRRAPEGARSPGTTPDDAGPSAGQRGSVT
                     NSSA"
     gene            complement(638..2386)
                     /locus_tag="PLB86_11770"
     CDS             complement(638..2386)
                     /locus_tag="PLB86_11770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC65863.1"
                     /translation="MPPAPLPADPSASPAVTYPDCVPELSDGVVTLRAHRPQDAERIV
                     QQCVDGDSQAFLPLPSPYGLPEAQAFLDKVAAAWLDEWGAREWAITDAADPSGQFLGS
                     INLHERSPYRAEVGFGLHPEGRGRGLVARAVRLLAARAFDEGAEVLRWRAVAGNWASR
                     RVAWACGFEAPTTLIGGRLDSDGHPGDEWHAVLRPGEPMAPRTPWLVAPDVARGRIRL
                     REFRDDDEHRLPAERDDRAARFLTRGMPTRSRYAAWLTEQRSAAAMGDTVQWALADRH
                     TDEVLGAIVLTRQAHPRTRGTAVVGYWLLPQWRGQGLMTEGLDLAVDTAFAPKPEGLG
                     LRALRAVADATNTESARVLRAAGFVSGGRERAAYTHVDEPPADAVTFDVLVSDDREAQ
                     RVRPLPIPVIETERLRLRPWRETDRPDPADDPDEAVRRFIPAGAAPGHADFDVWLARK
                     RRFNDEGSLVEWCLADRETDRALGSVGLFTRGDGPIDFNVEIGYFIFPTARGHRYVGE
                     ALPHVIEHAFRPRSEGGLGVSRVHAGADTDNDASRAILRAAGLREWGHDRQAWRRADG
                     SLSDGSYAELLVTDPR"
     gene            2516..3586
                     /locus_tag="PLB86_11775"
     CDS             2516..3586
                     /locus_tag="PLB86_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRC65864.1"
                     /translation="MSDVPPSAPQPSRGRHRARGQALAPSALAGAHVTRRVAMAAFGG
                     ALVGGLVEVLTDRSPGSAPAAQTPVATPTTSQVTTAARATVPPATATVTASATSPVAT
                     ATPTASSPPTSVEGGFAEHGALVAALTALPRGKNVAFGVGVLDAATGRRFVFDVGAPF
                     EMASTVKVDILVATFLRAADAGRGLTAGEKSLASVMIRNSDNAAASTLFRAAGRAPGM
                     AKAYQRMGMSATKASSAWGLTTTTPGDRLVVLDALARGGGALTAEAGAEILALMRSVT
                     TSQRWGVGGVARAGETVAVKNGWLPRSNQGGRWVISTSGLLTGPSTDLRLAVCSRGHA
                     SQAAGIDFVEKVLTLARGELKV"
     gene            3717..>4246
                     /locus_tag="PLB86_11780"
     CDS             3717..>4246
                     /locus_tag="PLB86_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRC65865.1"
                     /translation="MAKVFEKLLRAGEGRVVKRLEAVAAQVNILEEDFEKLTDAELRA
                     ETDTFKRRLAEGESLDAILPEAFAAVREASKRTLGKRHFDVQIMGGVALHQGNVAEMR
                     TGEGKTLVATLPTYLNALAGKGVHVITVNDYLAEYQSELMGRIHRMLGLETGCILSAM
                     TPDQRRAEYAKDITYGT"
BASE COUNT          576 a         1484 c         1550 g          636 t
ORIGIN      
        1 tgcagaccct cggggaggcg agccaccgac tgtcgttctg gcttctggcc gggacgttct
       61 tcatctgcgg ttggtcgacg aacggcctca tcggcaccca cttcatccct gcggctcacg
      121 accacgggat gccgccgacg accgcggcgg ggttgctcgc cctgatcggc atcttcgaca
      181 tcgccgggac ggtcgcctcg ggctggctga ccgaccgggt ggacccgcgg ctgctgttgg
      241 tgggctacta cgtcctgcgg ggcctgtcgc tgctcgtcgt cccgtcggtc ctcgggccgg
      301 gagtcgagcc gagcctgttc ctcttcatcg tcttctacgg gttggactgg gtggcgaccg
      361 tcccaccgac gatggcgttg tgccgggcgc acttcgggct ggaacgctcg agcatcgtgt
      421 tcggctgggt gttcgccgcg cacatggtcg gggccgggat cgcggcgacc ttcgcgggct
      481 gggtgcgcca gtccagcggc gactacttct gggcgtggct caccgccgga atcctctgtc
      541 tcattgccgg attcgcctgc ttggcgatcc cgcgccgggc acccgaaggc gctcgctcgc
      601 ctggaaccac cccagatgac gcggggccgt ccgccggtca gcgcggatcg gtgaccaaca
      661 gttcggcata gctcccatcg ctcagggagc cgtcggcccg ccgccacgcc tgccggtcgt
      721 ggccccactc gcgcagcccg gcggcgcgca ggatcgcccg tgaggcgtcg ttgtccgtgt
      781 cggcccccgc atggacgcgg ctcacgccga ggccgccttc cgatcgcggc cggaaggcgt
      841 gctcgatgac gtgggggagc gcctcaccga cgtagcggtg cccgcgcgcc gtcgggaaga
      901 tgaagtagcc gatctcgacg ttgaagtcga tcggtccgtc accgcgggtg aagagtccca
      961 cgctgcccag cgcccggtcg gtctcgcgat cggcgagaca ccactccacc aggctgcctt
     1021 cgtcgttgaa ccggcgcttg cgtgcgagcc acacgtcgaa gtcggcgtgc ccgggagccg
     1081 caccggcggg gatgaagcgc cgcacggctt cgtcgggatc gtcggcaggg tcgggccggt
     1141 cggtctcccg ccacggccgc aggcgaagtc gctcggtctc gatgaccggg atcggcaagg
     1201 gccgcacgcg ctgggcctcc cggtcgtcgc tcaccaacac gtcgaaggtg accgcgtcgg
     1261 cggggggctc gtccacatgg gtgtatgccg cccgctcccg gccgccactg acgaagccgg
     1321 ccgcacgcag cacccgagcc gactcggtgt tggtcgcgtc ggccactgca cgcagggccc
     1381 gcagccccag gccctcgggc ttcggcgcga acgcggtgtc gaccgcgagg tccaggccct
     1441 cggtcatcaa accctggccg cgccactggg ggagcagcca gtagcccacg accgccgtgc
     1501 cccgagtgcg cggatgcgcc tgtcgggtga ggacgatcgc ccccaggacc tcgtcggtat
     1561 gccggtcggc cagcgcccac tggacggtgt cgcccattgc ggcggcggag cgctgttcgg
     1621 tgagccacgc ggcataccgg ctgcgggtgg gcatgcccct ggtgaggaac cgggccgcgc
     1681 ggtcgtcgcg ttcggccggc agccggtgct cgtcgtcgtc gcggaactct cgcagccgga
     1741 tccggccccg agcgacgtcg ggggcgacca accagggggt gcgcggggcc atcggctcgc
     1801 cggggcgcag cacggcatgc cattcgtcgc cgggatgccc gtcgctgtcc agtcgtcccc
     1861 cgatcagggt cgtcggggcc tcgaagccgc aggcccacgc caccctgcgg gaggcccagt
     1921 tgccggcgac cgctcgccac cggagcacct ccgctccctc atcgaaagct ctggccgcca
     1981 acagtcggac cgcccgggcg accagcccgc gcccgcgtcc ctcggggtgc agcccgaaac
     2041 cgacctcggc gcgatagggg ctgcgctcgt gcaggttgat gctgccgagg aactgcccgc
     2101 tcgggtcggc cgcgtcggtg atggcccatt cccgggcgcc ccactcgtcg agccaggccg
     2161 cagcgacctt gtcgaggaag gcctgcgcct cgggcaaccc gtagggggag ggaaggggca
     2221 ggaaagcctg gctgtcgcca tccacacact gctgcacgat gcgctcggcg tcctgcggtc
     2281 ggtgcgcgcg cagggtgacg acgccgtcgg acaactccgg gacgcagtcc gggtaggtca
     2341 ctgcgggcga agcggagggg tcggcaggaa ggggcgcggg cggcataccc caagggtgtc
     2401 gagccggtat gccgttgagc aaccgctgtg ctcaaccgca gtggccgagg gctgactggg
     2461 gtgctgggtt ggccccgagg cgcgccgaca cagcaggtgt cgccatactt gcgccgtgtc
     2521 cgacgtgccg ccctccgcgc cgcagccgtc ccgggggcgc catcgcgccc ggggtcaggc
     2581 gctcgctccg tcggctctgg caggcgccca cgtgactcgt cgcgtcgcga tggccgcctt
     2641 cggcggtgcg ctggtgggcg ggcttgtcga agtgctcacc gatcggtcgc cgggatcggc
     2701 cccggccgcg cagacgccgg tcgccacccc gacgacctct caggtcacga cagcggcccg
     2761 ggcaacggtg ccgcccgcga ccgcgacagt gactgcttcg gcgacgagcc cggtggcaac
     2821 agccaccccg acggcctcct ctcctcccac gtcggtcgag ggcgggttcg ccgagcacgg
     2881 cgctctcgtc gccgccttga ccgccttgcc gcgggggaag aatgtcgcct tcggcgtcgg
     2941 cgtcttggac gccgcgaccg ggcgacggtt cgtcttcgac gtgggagcgc cgttcgagat
     3001 ggccagcacg gtcaaggtgg acattctcgt ggcgacgttc ctacgcgccg ccgacgcagg
     3061 tcgggggctc acggctggcg agaagagcct cgcgtcggtg atgatccgca actccgacaa
     3121 cgcggcggca tcgacactgt tccgcgcggc cggacgggcg ccggggatgg ccaaggccta
     3181 ccagcgcatg gggatgtcgg cgacgaaggc ctcgtcggcg tggggcctga caacgacgac
     3241 gccgggcgac cggttggtcg tcctcgatgc cttggcgcga ggtggtgggg cgctgacggc
     3301 cgaggccggc gccgagatcc tcgcgctgat gcgctcggtg acgaccagcc aacggtgggg
     3361 cgtgggcggg gtggcccggg ccggtgagac ggtcgcggtc aagaacggct ggctgcctcg
     3421 gagcaaccag gggggtcgat gggtcatttc gacctccggc ctgctcaccg ggccctcgac
     3481 cgacctgcgg ctggccgtgt gcagccgggg gcacgccagc caggccgccg gtatcgattt
     3541 cgtcgagaag gtgctgacgc tggcgcgcgg cgagttgaag gtctgaccgg ttcgcacgtg
     3601 cgcggaacca gggcgacggc aagaggcgtt ggtgaggtga cctgagagcc cgggcactac
     3661 cctgggtctc tggctctacg tgagccgacc gccgttcgtc gttcgaggag tttcttgtgg
     3721 ccaaggtgtt cgagaagttg ctgcgtgccg gcgagggccg ggtcgtcaag cggctcgaag
     3781 cggtggcggc ccaggtcaat atcctcgagg aggacttcga gaagctgacc gacgccgagc
     3841 tgcgcgccga gaccgacacg ttcaagcggc gcctggctga gggcgagtca ctcgacgcga
     3901 tccttcccga ggccttcgcc gcggttcggg aggcgtccaa gcggaccctc ggcaagcggc
     3961 acttcgacgt gcagatcatg ggcggggtcg ccctgcacca gggcaacgtc gccgaaatgc
     4021 gcaccggtga gggcaagacc ctcgtcgcga ccctgccgac ctacctcaac gcgctcgcgg
     4081 gcaagggcgt tcacgtcatc acggtcaacg actacctggc cgagtaccag tcggagttga
     4141 tgggccgcat ccaccggatg ctcgggctgg agaccggctg catcctctcc gcgatgaccc
     4201 ccgaccagcg ccgcgccgaa tatgccaagg acatcaccta cggcac
//
LOCUS       DAORPW010000203         4238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6431_length_4238_cov_546.380827, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000203 DAORPW010000000
VERSION     DAORPW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6431_length_4238_cov_546.380827"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..>1338
                     /locus_tag="PLB86_11785"
     CDS             179..>1338
                     /locus_tag="PLB86_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC65866.1"
                     /translation="MTTSTLDRHGGLVTGSRGGLELKGWRRRFPWSVAGHLGLGLLAG
                     VCAPLVLVSVLGAMVIGRVPVLTARGEALLGLALRWPARVEAVRFADFLGVVIEVPPP
                     DGQRGPLPASGARDRALTQRERATVGYWLARPIVSLAELATLVPVLGLPIVVAVAFAL
                     GWPEWRLEVGIGSGISQPEATLAWLVVGVVLLLAWLRLVGAATVAALGRAEVKLALWL
                     LGGREAALVERVEALATSREVVVAAAEEERQRIERDLHDGAQQRLVALTMLLGRAQAR
                     LGEREPDVADLIAQAKAESRAATAELRDLTRGLHPPVLTDRGLDAALSAIAARSPVPV
                     EVMVDLPVRPPMAVEATLYFAVAEALTNVAKHSGAAQAVVTVRHVGNVVLTEV"
     assembly_gap    1339..1438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1439..1941
                     /locus_tag="PLB86_11790"
     CDS             <1439..1941
                     /locus_tag="PLB86_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC65867.1"
                     /translation="DVRMPPAYLDEGLRAALVIRQRWPEVGVLVLSQYVEERYAVELL
                     SDRPGGVGYLLKDRVGDVLEFVDALRRVARGGTALDPDVVAQLLVRARHTDPLASLTA
                     RERQVLGAMAQGRSNAGIAADLLIGEGAVEKNVSSIFGKLGLYADTGEHRRVLAVLRF
                     LGDGRE"
     gene            1934..2497
                     /locus_tag="PLB86_11795"
     CDS             1934..2497
                     /locus_tag="PLB86_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65868.1"
                     /translation="MSETTSVRPNRARLGVLRVAGLLVLMVSLVLPATRAADALNIRQ
                     RIDHYQEFDQLSAQVSQVTINAAGTWVEVVRGENPLDAGVVWVQRACVLPSSVVPWSG
                     VSTADESEGEPPNDGHLLVFASSITGWEQALRRCETWVHVYVDPRMAVRVWDGSQVHE
                     VDTDLARWTLSPDGALVEHLLLGNPGG"
     gene            complement(2525..3397)
                     /gene="fdhD"
                     /locus_tag="PLB86_11800"
     CDS             complement(2525..3397)
                     /gene="fdhD"
                     /locus_tag="PLB86_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRC65869.1"
                     /translation="MSRVTRRVRTTRYAVGAPPRVREDHVAAEEPLQIRLRSWAGEHR
                     DLTVTMRTPGDDFDLVAGFLVGEGMIRSADDLATMRYCAGTDESGEQTFNVIDATLAA
                     GVALPPSRVERQVTTTSACGICGTTSIEDVLTAAPGWPLDPTTVPADLLFGLPDRLRV
                     AQSLFERTGGVHAAGLFRVGDVDEVGEAGEDSGLLCLREDVGRHNAVDKVIGWALREG
                     RLPLTGTVLQVSGRASFELVQKAWQGGIPVLAAVGAPSSLAVELATEAGLTLVGFSRG
                     ASFNVYTHPERVSS"
     gene            3474..4208
                     /locus_tag="PLB86_11805"
     CDS             3474..4208
                     /locus_tag="PLB86_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HRC65870.1"
                     /translation="MKPFLLLAIRAEDAAADDEYASILRLGGLDPSEVHRVRLEQASG
                     GDLDPTEFSGVILGGGPFQRSDPEATKSKVQLRVEAELETLLDVVVGEDLPFLGLCYG
                     IGVLGTHQGGIVDRAFGEAVGRASVIVTEAGRADPLFSGLPEEFEAFGGHKEALSVVP
                     RHATVLATSAACPVQAFRVGANVYATQFHPELDSASLQTRIDAYRDYGYFPPEEAESL
                     KAVARERDVEWPAMILRRFVRRYAAR"
BASE COUNT          565 a         1398 c         1493 g          682 t
ORIGIN      
        1 caggagatgg ccgagtatgc cgcccgcctc cgcgccgccc tttagcgcgc cgtcctctag
       61 ggcgccgtcc ggctgctgct gacggggtgg cccccgggga cgaggggggc cagccccctg
      121 ggggtggggt cgccgggtcg ttagcgtgga agagacgcga cgacgggatg gactcacgat
      181 gacgacctcg accctggacc ggcatggggg cctcgtgacg ggttcgcgag ggggtcttga
      241 gttgaaggga tggcgccgcc ggttcccgtg gagtgtcgcc ggccacctcg ggctcgggct
      301 gctcgcgggt gtctgcgccc cgctcgtgct ggtgtcggtg cttggggcga tggtcatcgg
      361 tcgcgtcccc gtcttgactg cacgaggtga ggcgttgctt gggcttgcgc tgcgctggcc
      421 ggctcgggtg gaggcggtcc gcttcgctga cttcctgggt gtggtcatcg aggtaccgcc
      481 gccggacggg caacgggggc ctttgcccgc ttcgggagca cgagaccggg ccctgactca
      541 gcgggagcgt gccaccgtcg gctattggct ggcccgaccg atcgtctcgc tcgcggaact
      601 cgcgaccctc gtccccgtcc ttgggctccc gattgtcgtg gccgtggcct tcgcgctcgg
      661 gtggcctgag tggcgcctgg aagttggcat cggcagtggg atttcccagc ccgaagccac
      721 cctggcctgg ctggttgttg gggtggtcct cctgctggcc tggcttcgac tggtgggcgc
      781 cgcaacggtc gctgccctgg gaagggctga agtgaagttg gcgctctggc ttctcggagg
      841 ccgagaagcc gcactcgtag aacgcgtcga ggcgctcgcg acgagccgcg aagtcgtggt
      901 ggcggcggcc gaggaggagc gacagcgcat cgagcgcgac ctccatgacg gtgcccaaca
      961 gcgactcgtg gcgttgacga tgctgctcgg cagggcgcag gccaggctcg gcgaacgcga
     1021 gcccgacgtc gctgacctca tcgctcaggc caaggccgaa tctcgagcgg cgacagccga
     1081 gttgcgagat ctcacccgtg ggctgcaccc gccggtcctc accgaccgcg ggctggacgc
     1141 ggccctgtct gctattgcgg cgcgttcgcc ggtgcccgtt gaggtcatgg tcgacctgcc
     1201 tgtccgtcca cccatggcgg tggaagccac cctctacttc gccgtggccg aggcgctgac
     1261 caacgtggcc aaacactcag gcgccgcgca ggcagttgtc acggtccgcc acgtcggcaa
     1321 cgtggtgctg accgaggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc
     1441 gacgtgcgga tgccaccggc atacctcgat gagggcttgc gtgctgcgtt ggtgatccgg
     1501 cagcggtggc ccgaggtggg cgtgctggtg ctctcgcagt atgtcgagga gcggtatgcc
     1561 gtcgagttgc tgtcggaccg gccgggcggg gtgggctacc tgctgaagga ccgggtgggc
     1621 gacgtgctcg agttcgtcga cgcgctgcgc cgagtggctc gcggagggac tgccttggac
     1681 cccgacgtgg tcgcgcaact attggtgcgg gcacggcata ccgacccgct ggcgagcctg
     1741 acggcccgcg agcgacaggt ccttggggcg atggcccagg gccgctccaa cgccggcatc
     1801 gccgcggacc tactcatcgg cgaaggcgcc gtcgagaaga acgtgtcgag catcttcggc
     1861 aagctcggcc tctatgccga caccggcgag caccgtcggg tgctcgccgt cctgcgcttc
     1921 ctgggagacg gccgtgagtg agacgacatc ggtgcgcccc aaccgggctc ggctcggagt
     1981 gcttcgggtt gccggacttc tggtgctgat ggtgagcctg gtgctgcccg cgaccagagc
     2041 cgcggacgca ttgaacatcc gacagagaat cgaccactac caagagtttg accagttgtc
     2101 cgcgcaggtc tcacaggtca cgatcaatgc ggccggcacg tgggtcgagg tcgtccgcgg
     2161 cgagaacccg ttggacgctg gagtggtctg ggtgcagcgc gcgtgcgtgc tgccctcaag
     2221 cgtcgtgccc tggtccggtg tctccaccgc cgacgagtct gagggcgagc ctccgaacga
     2281 cgggcacctc ctggtgtttg cctcctcgat caccggttgg gagcaagcac tgcggcgatg
     2341 cgagacgtgg gtccatgtct acgtggatcc tcggatggcc gtgcgcgtgt gggacgggtc
     2401 ccaggtccac gaggtcgaca ccgacctggc gcggtggacg ctcagtccgg acggcgcatt
     2461 ggttgaacac ctgctattgg ggaatcccgg aggctgatgg gtgccgggtg ctgacgcccc
     2521 gctgttagct ggagacccgc tcggggtggg tgtagacgtt gaagctggcc ccgcggctga
     2581 acccgacgag ggtcaggccg gcctcggtgg cgagttcgac ggccagcgag gagggcgctc
     2641 ccaccgccgc cagcaccggt atgccgccct gccacgcctt ctgcaccaac tcgaacgagg
     2701 cgcgtccgct gacctgaagg accgtgccgg tcaacggcaa ccgcccctcg cgcagcgccc
     2761 agccgatcac cttgtcgacc gcgttgtgcc gcccgacgtc ctcgcgcagg cacagcaacc
     2821 ccgagtcctc accggcctct ccgacctcat cgacgtcgcc gaccctgaac agccccgccg
     2881 cgtggacccc gcccgtccgc tcgaacagtg actgcgcgac ccgcagccga tcgggcaacc
     2941 cgaacagcag atccgcaggc acggtcgtcg gatccagcgg ccaccccggc gccgcagtca
     3001 acacgtcctc gatcgacgtc gtcccgcaga tcccgcacgc gctcgtcgtc gtcacctgcc
     3061 gctcgacccg gctcggcggc agcgctaccc cggccgcgag agttgcgtcg atgacgttga
     3121 aggtctgctc gcctgactcg tcggtccccg cgcagtagcg catcgtcgcc aggtcatccg
     3181 ccgaccgaat catcccctca cccacgagga agcccgccac caggtcgaag tcgtcccccg
     3241 gcgtgcgcat cgtcacggtg agatcccggt gctccccggc ccacgatcgc agccggatct
     3301 gcaggggttc ctccgccgcg acgtggtcct cgcggacccg aggaggtgcg cccacggcat
     3361 accgggtcgt ccgcacccgc cgcgtcaccc gactcatgag tggacacggt atgccgtcca
     3421 gcccgcgggc ggggcgcgcg cggacggcat acccgcgcct aacctgggtg gccgtgaagc
     3481 ccttcctgct gctggccatc cgcgccgagg acgccgcggc ggacgacgag tatgcgtcga
     3541 tcctgcgtct tggcgggctc gacccgagcg aggtgcatcg ggtgcgcctc gagcaggcgt
     3601 cgggtggcga cctcgacccg acggagttca gcggggtcat cctcggcggc gggccgttcc
     3661 aacgcagcga ccccgaggcg acgaagtcca aggtgcaact gcgggtcgag gccgagctcg
     3721 agacgctgct cgatgtggtg gtgggcgagg atctgccgtt cctcgggctg tgttacggga
     3781 tcggtgtgct ggggacgcat cagggtggca tcgtcgaccg ggccttcggt gaggcggtcg
     3841 gtcgggcatc ggtgatcgtc acggaggctg ggcgggccga tccgttgttc tcggggctgc
     3901 ccgaggagtt cgaggcgttc ggcggacaca aggaggcgct gtcggtggtg ccgcggcacg
     3961 cgacggtgct cgcgacgtcg gccgcctgcc cggtgcaggc attccgggtc ggcgcgaatg
     4021 tctatgccac gcagttccac cccgagcttg actcggcgag cctgcagacg cgcatcgatg
     4081 cctaccggga ctacgggtac ttcccgcccg aggaggccga gtctctcaag gcggtcgcga
     4141 gggagcggga cgtcgaatgg ccggcgatga tcctgcgccg cttcgtccgc cggtatgccg
     4201 cccgctgacc tctgcgctga cccctcgctc gccggtcc
//
LOCUS       DAORPW010000204         4228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6454_length_4228_cov_830.217350, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000204 DAORPW010000000
VERSION     DAORPW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6454_length_4228_cov_830.217350"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLB86_11810"
     CDS             complement(<1..422)
                     /locus_tag="PLB86_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65871.1"
                     /translation="MRTYLLLGRDAEALAACSRALADNPTDTGLLTLSSIAAYRLGQH
                     DDAIRFAMMAISAGPDSDSAHRALATALLTVGRAQEATSAAYTAAALAPHNWINHLRY
                     SQCLMAMPDSRDAAWQAATRAVELAPHESLAHYQVACVV"
     gene            complement(465..>1345)
                     /locus_tag="PLB86_11815"
     CDS             complement(465..>1345)
                     /locus_tag="PLB86_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC65872.1"
                     /translation="AWDVERATVRLADVGGMREVKERILVSFLAPMQNPEMRKLYGKS
                     LRGGILLYGPPGCGKTFIGRAIAGEMGAQFINVGLSDVLDMYIGQSERNLHELFQIAR
                     RNAPVVIFLDEVDALGQKRSLTRHSAMRGTVNQLLAELDGVSDSNEGVYVVGATNQPW
                     DVDSALRRPGRFDRTLLVLPPDQEAREAILGYHLKDRPIEGINLASLAKATDGYSGAD
                     LAHICESAAERAMMEGIRSGTVRMIRMDDLYAALREVRPSTGPWFDAARNVVQFANTD
                     GTYDELRTYMKRTKKL"
     assembly_gap    1346..1445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1586..4207)
                     /gene="clpB"
                     /locus_tag="PLB86_11820"
     CDS             complement(1586..4207)
                     /gene="clpB"
                     /locus_tag="PLB86_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRC65873.1"
                     /translation="MDLKLTTKAQEALSAAVRASAAAGNPHTEPAHLLKALTEQSDTT
                     TPALLEAAGSSLQAASRVAEQALSGMPSVSGSSVSAPGLSRSAMAVLEAARAAMAAMG
                     DTYVSTDHLLLALAKTGRFGLDARAIETAIPTLRGGRSVDSENPEGTFDALTKYGTDL
                     TARAREGKLDPVIGRDAEIRRVVQVLSRRTKNNPVLIGEPGVGKTAVVEGLAQRIVDG
                     DVPESLRGKKLISLDLGAMVAGAKYRGEFEERLKAVLEEITNSEGQVVTFIDELHTVV
                     GAGASGEGAMDAGNMLKPLLARGELRLVGATTLDEYRKHIEKDPALERRFQQVYVGEP
                     SVEDTIAILRGLKERYEAHHKVEIEDSALVAAATLSDRYITGRQLPDKAIDLIDEAAS
                     RLRMEIDSSPVEIDQLQRTVDRMRMEELHLAKETDAASVARLERLRKDLADKTDELAA
                     LNARWEAEKSGLNRVGELKVRLDDLRTQADRLQREAQYEDAARLLYGEIPALEKELAS
                     AQETEAAHRAGAGVAGSPEGAAGAGAGGTAYKDVMVKDRVGADDIAEVISAWTGIPAG
                     RLLQGETEKLLHMEQLIGARLIGQADAVRAVSDAVRRSRAGLSDPDRPTGSFLFLGPT
                     GVGKTELAKSLADFLFDDERAMVRIDMSEYSERHAVARLIGAPPGYVGYEEGGQLTEA
                     VRRRPYSVVLLDEVEKAHPETFDILLQVLDDGRLTDGQGRTVDFRNVILVMTSNIGSQ
                     FLVDPMLTDEAKREAVMGMVRASFKPEFLNRLDEVVIFDPLSTEELARIVELQVRALS
                     ARLADRRITLEVTEAAKEWLAVTGYDPAYGARPLRRLVQREIGDRLARALLAGEVRDG
                     STVTVDRDDEADSLVLR"
BASE COUNT          637 a         1423 c         1376 g          692 t
ORIGIN      
        1 accacgcagg ccacctggta gtgcgccagc gactcgtgcg gagccagttc cacagcccga
       61 gttgccgctt gccaggcagc atcgcgactg tcgggcatgg ccatgaggca ctgtgagtag
      121 cgcagatggt tgatccagtt gtgcggcgcc aacgcagcag cggtgtatgc cgcggacgtc
      181 gcctcctgcg ccctgcccac agtgagcagg gctgtcgcca acgctcgatg cgcggagtcg
      241 gagtcggggc ctgcggagat cgccatcatg gcgaaccgaa tagcgtcgtc gtgctgcccg
      301 agacggtaag cggcgatgga cgacaaggtg agcaaaccgg tgtcggttgg gttgtcggcg
      361 agggcccggc tgcacgctgc gagcgcctcc gcgtcgcggc caagcaagag ataggtccgc
      421 acccgttcgc ttgggtccgg cgtggcgttc ctggccattc gccctcagag tttcttcgtg
      481 cgcttcatat acgtgcgcaa ctcgtcgtag gtcccgtcgg tgttggcgaa ctgcacgacg
      541 ttgcgcgctg cgtcgaacca cggcccggtc gacgggcgga cctcgcggag ggcggcatac
      601 aggtcgtcca tccggatcat tcgcacggtg ccgctgcgga ttccctccat catcgcccgc
      661 tcggcggcac tctcgcagat atgggcgagg tcagccccgg agtagccgtc ggttgccttg
      721 gccagggaag cgaggttgat gccctcgatg ggtcgatcct tgaggtggta gccgaggatc
      781 gcctcccgag cctcctggtc gggaggcagc accagaaggg tccgatcgaa ccgcccgggc
      841 cggcgcagcg cggaatcgac gtcccacggc tggttggtcg cgccgacgac gtagaccccc
      901 tcgttgctgt ccgacactcc gtccagctcg gcgagcaact ggttgaccgt tccgcgcatc
      961 gccgagtgcc gggtcaggct gcgcttctgg cccagcgcat cgacctcgtc gaggaagatg
     1021 acgacggggg cgttgcgtcg agcgatctgg aacagctcgt gcaggttgcg ctcggactgg
     1081 ccgatataca tgtccagcac gtcggacaag cccacgttga tgaactgcgc acccatctct
     1141 ccggcgatgg cccgcccgat gaacgtcttc ccacatcccg gcggcccata gagcaggatg
     1201 ccgccgcgca gactcttgcc ataaagcttg cgcatctccg ggttctgcat cggcgctagg
     1261 aacgacacga ggatgcgttc cttgacctcg cgcatcccgc ccacatcggc cagtcgcacg
     1321 gtcgctcgct cgacgtccca ggcacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnttcgc caacgatgcg atgagcggat tcgtcatgcc gtgatcgtag ggccgctccc
     1501 cacccccgcc gggtcgaggt atttcgccat cgtcaggaga tagccgaata cctcgacccg
     1561 gcaaaggaaa ccgagcagag aaccgtcagc gcaggacgag cgagtcggcc tcgtcgtcgc
     1621 gatcgacggt gaccgtcgac ccgtcccgga cctcgccggc caacagcgcc cgagccaacc
     1681 ggtcgccgat ctcgcgctgc accaaccggc gcaacggacg agccccataa gcagggtcgt
     1741 aacccgtgac cgccagccat tccttggcgg cctcggtgac ctccagcgtg atccgccggt
     1801 cggccaaccg cgccgacagc gcccgcacct gcaactcgac gatccgcgcc aactcctccg
     1861 tcgacagcgg gtcgaagatg acgacctcgt cgagccggtt gaggaactcg ggcttgaacg
     1921 acgcccgcac catccccatg acggcctcgc gcttggcctc atcggtcaac atcgggtcga
     1981 cgaggaactg gctgccgatg ttggaggtca tgacgaggat gacgttgcgg aagtcgaccg
     2041 tgcgcccctg cccatcggtg agccgcccgt cgtcgagcac ctgcaggagg atgtcgaagg
     2101 tctccgggtg ggccttctcg acctcatcga gcagcacgac ggagtaaggc cgacgccgca
     2161 ccgcctcggt gagctgccca ccctcttcgt aacccacgta gcccggaggc gcaccgatga
     2221 gccgcgcgac cgcgtgccgc tcggagtact ccgacatgtc gatccgcacc atcgcgcgct
     2281 cgtcgtcgaa gaggaagtcg gccagcgatt tggccagctc ggtcttgccc acgccggtcg
     2341 gcccgaggaa gaggaacgac ccggtcggcc gatcggggtc ggacagacct gcgcgcgagc
     2401 gccgcactgc gtcgctgacg gcccgcaccg cgtcggcctg gccgatgagg cgcgcaccga
     2461 tgagctgctc catgtgcagc aacttctcgg tctcgccctg cagcagccgc ccggccggga
     2521 tgccggtcca cgccgagatc acctcggcga tgtcgtcggc accgacgcgg tccttgacca
     2581 tgacgtcctt gtatgccgtc ccgccggctc ccgcgcccgc cgcaccttcg gggctgccgg
     2641 caactccagc accggcgcgg tgggccgcct cggtctcttg agcgctggcc aactccttct
     2701 ccaatgcggg gatctcgccg tagaggagcc gagcggcatc ctcatactga gcctcgcgct
     2761 gcagccggtc ggcctgggtg cgcagatcgt ccagacgcac cttgagctcg ccgacccggt
     2821 tgaggccgga cttctcggct tcccaacggg cgttgagcgc agccaactcg tcggtcttgt
     2881 cggccaggtc cttgcgcagt cgctccagtc gcgcgaccga cgcagcgtcg gtctccttgg
     2941 ccaggtgcaa ctcctccatc cgcatccggt cgacggtgcg ttggagttgg tcgatctcga
     3001 cgggtgagga gtcgatctcc atccgcagcc gcgaggcagc ctcgtcgatg aggtcgatcg
     3061 ccttgtcggg cagttggcga ccggtgatgt agcggtcgga caaggtggcg gcagcgacca
     3121 gcgcagagtc ctcgatctcg accttgtggt gggcctcgta acgctccttg agcccccgca
     3181 ggatcgcgat ggtgtcctcg accgacggct cgccgacgta gacctgctgg aagcgccgct
     3241 ccagcgcggg gtccttctcg atgtgcttgc ggtattcgtc gagggtcgtc gcgccgacca
     3301 gccgcaactc gccgcgtgcc aacagtggct tgagcatgtt gccggcgtcc atggcgccct
     3361 cgccgctcgc gccggcaccg acgaccgtgt gcaactcgtc gatgaaggtg acgacctggc
     3421 cctcggagtt ggtgatctcc tcgaggacgg ccttgagccg ctcctcgaac tcgccgcggt
     3481 acttcgcccc cgcgaccatc gccccaaggt cgaggctgat gagcttcttg ccgcgcaggc
     3541 tctcgggcac gtcgccgtcg acgatccgct gggccagccc ttcgaccacg gcggtcttgc
     3601 cgacaccggg ctcgccgatg aggacggggt tgttcttggt ccgccggctg agcacttgga
     3661 cgacccgcct gatctcggcg tcacggccga tgaccgggtc gagcttgccc tcgcgggccc
     3721 gcgcggtgag gtcggtgccg tatttcgtca gggcatcgaa ggtcccctcg gggttctccg
     3781 agtcgacgct ccggccgccg cgcaacgtcg gaatggcggt ctcgatggcg cgggcgtcca
     3841 gcccgaagcg acccgtcttg gccagcgcca gcaacaggtg atcggtcgag acataggtgt
     3901 cgcccatggc ggccatcgca gcccgagcgg cttccagaac ggccatcgcc gatcgggaga
     3961 gccccggtgc gctgaccgac gaaccggaga cggacggcat accggacaac gcctgctcgg
     4021 ccacgcggga agcggcctgg aggctggaac cggctgcctc gagcagcgcg ggcgtcgtcg
     4081 tgtcggactg ctcggtcagt gccttgagca ggtgggcggg ctcggtgtgg gggttgccgg
     4141 cggcggctga cgcgcggacg gcagccgaca gcgcctcctg ggccttggtc gtcagcttga
     4201 gatccatggg tatgtcgtgt gctcctta
//
LOCUS       DAORPW010000205         4228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6455_length_4228_cov_829.392284, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000205 DAORPW010000000
VERSION     DAORPW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6455_length_4228_cov_829.392284"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3104)
                     /gene="glgB"
                     /locus_tag="PLB86_11825"
     CDS             complement(<1..3104)
                     /gene="glgB"
                     /locus_tag="PLB86_11825"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HRC65874.1"
                     /translation="MAEVLDATLTPGKIDLVAGWIGRQRWYAAKGQAPRLTRLRGFRF
                     DDPAGEVGIETLVLRDDATTPPTVYQVPLTYRGAPLVGAERALVGTMEHSVLGTRYVY
                     DGPHDPVYVAALWRFIQGRAQAQAAGVSNTVEPAFSGHTVPGGYAARSSDVTASRVLG
                     GEQSNTSIVCGVADLGPVIVKVFRTLSPGANPDVVLQPALAAAGCEHVPTTLGWVSGQ
                     WADGQEEGHLAFAQEFLAGTQDAWRVATESVAAGSDLTGGAAQLGEVVAQIHQILAEA
                     FPTRRPTAEDRVTLVTGLRERFRAAADEVPELVDFAAAVHSRYDQALQASWPSLQRIH
                     ADLHLGQVIAHPSRGWVVLDFEGEPMRPLAERAQPDLPMRDLAGMLRSFDYAAGAYLQ
                     AHPDADADAAWAWARRCSEQFLAGYRAHSPGEATYPELLDALVLDKALYELVYEARNR
                     PSWVPIPLTAVRELLADGERTAYPTALPPDPSPLPAERRTVMEPVPTAALVADYSAVD
                     AFLEGRHSQPHDLLGHHLGPGGLTITCYRPMAASVRAKLADGTLLDLPHVKGGIWSGT
                     ASAIEQSQDYRLLVNYGDGIDHEQDDPYRFAPTLGELDRHLFNEGRHEQLWTILGSHV
                     RTYPGPLGEVRGVSFAVWAPNATAVHVVGDFNGWDRLSHPMRNLQPSGVWELFLPGAQ
                     EGMNYQYLVRGQDGITRLKADPMARFAEVAPKQASIVWESHYQWGDDEWMAARAERDV
                     HHGPMSIYEVHVGSWRQGQSYAQLAEHLVNYVKDLGFTHVEFMPVSEHPYVPSWGYHV
                     TGYYSVSSRWGTPDEFRLLVDYLHRNGIGVILDWVPGHFATDPWALAKFDGQALYEHP
                     DPRKGWHNEWGSLIFDFARKEVRNFLVANATYWLEEFHVDGLRVDGVASMLYLDYSRQ
                     PGEWVPNEFGGRENLDAVALLQEANATAYKRTPGVVTIAEESTSWGGVSHPTSMGGLG
                     FGFKWNMGWMNDALRYLATDPIYRQYHHNKLTFALMYAYSENFVLPISHDEVVHGKGS
                     LMRKS"
     gene            complement(3104..>4228)
                     /locus_tag="PLB86_11830"
     CDS             complement(3104..>4228)
                     /locus_tag="PLB86_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC65875.1"
                     /translation="EMFDVVRFWMDMGIDGFRLDAVPYLFEEEGHNGENHPKTHEFLA
                     QLRAMVDREYPGRILLAEANQLPAEVVDYFGTEQDPECHMCFHFPVMPRIYYSLRSQT
                     AAPIIDVLAATPAIPPGTQWGTFLRNHDELTLEMVSDDERAAMYRWYAPDPRMRANVG
                     IRRRLAPLLDNDRSQLELINALVLSLPGSPCLYYGDEIGMGDNIWLPDRDCSRTPMQW
                     NPDRNAGFSTADPGKLYLPVVSSLVFSYQAVNVESAQAAPASLLHWVRRILAVRKAHP
                     VFGLGDFVVTPADNPSILAYTRTAPDAATGARSVLCVANLSEHPQSASIDLGVDRAGQ
                     GLRDLFAGGAFPSIDAEGKARLTLGPRGYFWLAIDSSESA"
BASE COUNT          660 a         1338 c         1587 g          643 t
ORIGIN      
        1 gacttgcgca tgagcgagcc cttgccgtgg acgacctcgt catggctgat cggcaggacg
       61 aagttctcgc tgtaggcata catgagcgcg aaggtcagct tgttgtggtg gtactgccga
      121 tagatcgggt cggtggccag gtaacgcagg gcgtcgttca tccaccccat gttccatttg
      181 aagccgaagc ccagcccacc catcgaggtc gggtgcgaga ccccacccca cgacgtcgac
      241 tcctcggcga tcgtcacgac gccaggagtg cgcttgtatg ccgtggcgtt ggcttcctgg
      301 agcagggcca ccgcgtcgag gttctcgcgg ccaccgaact cgttggggac ccattcgccg
      361 ggctggcgcg agtagtcgag gtagagcatc gaggcgacgc cgtcgacccg cagcccgtcg
      421 acgtggaact cctcgagcca gtaggtcgcg ttggccacga ggaagttgcg cacctccttg
      481 cgggcgaagt cgaagatgag agagccccac tcgttgtgcc agcccttgcg ggggtcgggg
      541 tgctcataga gcgcctgacc gtcgaacttc gccaaggccc acgggtcggt cgcgaagtgg
      601 ccgggcaccc agtcgaggat gacgccaatt ccgttgcggt gcaggtaatc caccaacagg
      661 cggaactcgt cgggcgtgcc ccaacgcgac gacacggagt agtagccggt gacgtggtag
      721 ccccacgacg gcacgtaggg atgctcgctg acgggcatga actcgacgtg ggtgaagccg
      781 aggtccttga cgtagttgac gaggtgctcg gccaactggg cgtagctctg gccctgccgc
      841 cacgacccga cgtggacctc gtagatgctc atcggcccgt ggtgcacgtc gcgttcggcg
      901 cgggcggcca tccactcgtc gtcgccccac tggtagtgcg actcccagac gatcgacgcc
      961 tgcttggggg ccacctcggc gaaccgggcc atcgggtcgg ccttgagccg ggtgatgccg
     1021 tcctgaccgc gcacgaggta ttggtagttc atcccctcct gcgcgccggg gaggaagagc
     1081 tcccagacac ccgagggctg caggttgcgc atggggtggg agagccggtc ccaaccgttg
     1141 aagtcgccaa cgacgtggac ggcggtcgcg ttgggcgccc acacggcgaa ggacacgcca
     1201 cggacctcgc cgaggggccc ggggtaggtg cgcacgtgcg agccgaggat cgtccagagc
     1261 tgttcgtggc ggccctcgtt gaacaggtgg cggtcgagct cgccgagcgt cggggcgaac
     1321 cggtaggggt cgtcctgctc gtggtcgatg ccgtcgccgt agttgaccag caggcggtag
     1381 tcctggctct gctcgatggc gctggcggtg cccgaccaga tgccgccctt gacgtggggg
     1441 aggtccagca gagtgccgtc ggcgagtttg gcgcgcacgc tggcggccat cggccgatag
     1501 cacgtgatcg tcagaccacc cgggcccagg tgatgcccaa gcaggtcatg cggctgcgaa
     1561 tgccggccct ccaagaaggc gtcgacggcg ctgtagtcgg ctacgagcgc ggcggtcggg
     1621 acgggttcca tgacggtcct tcgttcggcg ggaaggggac tggggtcggg gggcagggcc
     1681 gtggggtatg ccgtccgctc accgtcggcg agcaactcgc ggacggcggt gagcgggatc
     1741 ggcacccaac tcgggcggtt gcgggcttcg tagacgagct cgtagagggc cttgtccagg
     1801 acgagggcgt ccaggagttc agggtaggtg gcctcgccag gtgaatgggc gcggtagccg
     1861 gcgaggaact gctcgctgca tcggcgagcc caagcccagg ccgcgtcggc gtcggcgtca
     1921 gggtgggcct gcaggtaggc gccggctgcg tagtcgaagg agcgcagcat gcccgcgagg
     1981 tcacgcatcg ggaggtcggg ttgggcgcgt tcggccagcg gccgcatggg ctcgccctcg
     2041 aagtccagga cgacccagcc cctggagggg tgggcgatga cctggccgag atgcagatcg
     2101 gcgtggatgc gctgcagcga cggccacgac gcctgcaacg cctggtcgta gcgggagtgc
     2161 accgccgccg cgaagtcgac caactcgggc acctcgtcgg cggccgcgcg gaagcgctcg
     2221 cgtaggccgg tcaccagggt gacccggtcc tcggcggtgg gccgtctggt cgggaaggcc
     2281 tcggcgagga tctggtggat ctgggccacc acctcaccca actgcgcggc cccaccggtg
     2341 agatccgagc cggcggccac cgactcggtc gccacgcgcc aggcgtcctg ggtgccggcg
     2401 aggaactcct gagcgaaggc cagatggccc tcctcctggc cgtccgccca ttggccgctc
     2461 acccagccca acgtcgtcgg cacgtgctcg caaccggcgg cggcgagggc gggctgcagg
     2521 acgacgtcgg ggttggcgcc cggcgacagc gtgcggaaca ccttgacgat caccgggccg
     2581 aggtcagcga ccccgcagac gatcgacgtg ttggactgct cgccccccag cacccgggat
     2641 gccgtgacgt cactggagcg ggcggcatac ccgccgggga ccgtgtgccc cgagaaggca
     2701 ggctcgacgg tgttgctcac gcccgcggcc tgggcctgcg cgcggccctg gatgaagcgc
     2761 cacagcgcgg cgacatagac agggtcgtgc gggccgtcgt agacgtatcg ggtgccgagg
     2821 acgctgtgct ccatggtgcc gacgagggcg cgttcggcgc ccacgagagg cgcaccgcga
     2881 taggtgagcg gcacctggta gaccgtcgga ggggtcgtcg cgtcgtcgcg caggacgagg
     2941 gtctcgatgc cgacctcgcc tgcggggtcg tcgaagcgga agccgcgcag tcgggtcagc
     3001 cgaggggcct gccccttggc ggcataccag cgctgcctgc cgatccaccc ggcgacgagg
     3061 tcgatcttgc cgggggtgag ggtggcgtcg aggacttcag ccatcaggcg ctctcggaac
     3121 tgtcgatcgc gagccagaag tagcccctcg ggccgagcgt cagtcgggcc ttgccctcag
     3181 cgtcgatcga ggggaacgcg ccgccggcga agaggtcgcg cagcccctga cccgcccggt
     3241 cgacgccaag gtcgatgctc gccgactgcg ggtgttcgga caggttggcg acgcacagca
     3301 ccgagcgggc gccggtcgcg gcgtcgggag cggtgcgcgt gtaggccagg atcgacgggt
     3361 tgtcggcggg cgtgacgacg aagtcgccga ggccgaaaac ggggtgggcc ttgcggaccg
     3421 cgaggattcg ccggacccag tgcagcagcg acgccggcgc ggcctgggcc gactccacgt
     3481 tgaccgcctg gtaactgaac acgagcgagg acacgaccgg gaggtagagc ttgccggggt
     3541 cggcggtgga gaagccggca ttgcggtcgg ggttccactg catgggggtg cgtgagcaat
     3601 cccggtcggg cagccagatg ttgtcgccca tcccgatctc atcgccgtaa tagagacacg
     3661 gtgacccggg cagcgagagc accaacgcgt tgatcaactc gagttgcgaa cggtcgttgt
     3721 ccagcagggg cgccagtcgc cgacggatgc cgacgttggc ccgcatccgg gggtcggggg
     3781 cgtaccagcg gtacatcgcg gcgcgctcgt cgtcgctgac catctcgagg gtgagctcgt
     3841 cgtggttgcg caggaacgtg ccccactggg tgcccggcgg gatcgccggt gtcgcggcga
     3901 ggacatcgat gatcggggcc gcggtctggc tgcgcaggga gtagtaaatg cgcggcatga
     3961 cggggaagtg gaagcacatg tggcactcgg ggtcctgctc ggtcccgaag tagtcgacga
     4021 cctccgccgg caactggttg gcctcggcga gcaggatccg cccggggtat tcgcggtcga
     4081 ccatggctcg cagctgggcg aggaactcgt gggtcttggg gtggttctcg ccgttgtgtc
     4141 cctcctcctc gaagaggtag ggcacggcat cgaggcggaa cccgtcgatc cccatgtcca
     4201 tccagaaccg cacgacgtcg aacatctc
//
LOCUS       DAORPW010000206         4202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6526_length_4202_cov_783.004340, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000206 DAORPW010000000
VERSION     DAORPW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6526_length_4202_cov_783.004340"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLB86_11835"
     CDS             complement(<1..189)
                     /locus_tag="PLB86_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65876.1"
                     /translation="MTDGRHRVALVTSSFHPHVGGVEEHVRQVALALRAQGHDVVVWT
                     VDRGEHLGVQTVDGVLVRY"
     gene            258..1307
                     /locus_tag="PLB86_11840"
     CDS             258..1307
                     /locus_tag="PLB86_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRC65877.1"
                     /translation="MPLLVLVTAAAAAPMGAQVYEEQLAARAQSALTAIDPQWQVQRR
                     IIRSMRSPLPGTGRLPMGWLAGAGERGRAAVGRLAYPRHAVVHRTSLELPPAPLEVVT
                     LHDVVAWRFADESAPVRSAAAELRRAAAVICVSEFSAGEAHDLLGVRNPVVVPNGVDP
                     AFLDAVAAPTEVLAGLGLTGPFVLHAGGAATRKNLDALAQAWPLVRREHPDLTLALAG
                     PPHPRRDALFSGMPGARLLGRLPDPLVPGLVAAASAIVVPSTYEGFGLPALEGMAAGV
                     PVVAARCAALPEVVGDGGLLVDPEPVALAQGLLDVLAGGPDVADLVRRGRERAGGFTW
                     ERSAAGHAAVWAALG"
     gene            1404..2432
                     /locus_tag="PLB86_11845"
     CDS             1404..2432
                     /locus_tag="PLB86_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRC65878.1"
                     /translation="MSDNPQPGEAHGERRRNRRLLVVVLLMLAVPVVAVGGYLGWLNH
                     LVTSNIKFADLMPTGGPLVPVDGGAFDPSEPGEGGIPTAAVPAESAGDNYLLIGSDAR
                     SGLGGARSDVIIFVHVPIDKHNVSLIHFPRDMYVFIPGHGRNKINAAFAFGGAPLLVD
                     TVQGMLGVKIDHVAMIGFEGFKRMTDAVGGLDVYVEEGGKIDGHVFTQGMMHLDGEMA
                     LAFVRERYDLSQGDISRGRRQQAFLKALMLEAVSVETLANPAKLATFIDSATSNVIVD
                     RGFDVGQMRGELLSLNGLRPGDIRFLTAPLSGFGRSPYGASIDLVDWVKLTQLGTAIR
                     EDRLDSYS"
     gene            complement(2466..3389)
                     /locus_tag="PLB86_11850"
     CDS             complement(2466..3389)
                     /locus_tag="PLB86_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65879.1"
                     /translation="MALLAVDVALVSAAFRNPFRQPATAPVTGAAATVTGTVPGSAGG
                     TSTPAATSSTPAATGFSGSGSMVGVVPIDSARAWRFVQGACPGGGASLAHTDNGGRTW
                     EDVPIPFGAISRVQVTDAAKVVVVGTEGAGCRETALRSTDTGQTWASGGALALWYRSL
                     ATPTAVVNSNGRAGTPCGNGKALSVSALSATSAAVLCESGKVVETADGGGNWVEVSRG
                     LSALAVDARVEASKLAALVAYVADGCSGVQVASIAAGATTKLGCVDLGGRSNDGLLTH
                     VALSVEGNAGWLIVDGDTYTSDDGGKTWSTP"
     gene            3577..>4202
                     /locus_tag="PLB86_11855"
     CDS             3577..>4202
                     /locus_tag="PLB86_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65880.1"
                     /translation="MTSTAPSAGTLAHLPTDWAARHTDWGLGAHVGDQTALLDLACVP
                     GARPLVLAVLAGALPVGAQVLAVTATTREADDLAAALAAFLDPALVEVFPSWETLPHE
                     RLSPRNDTVGRRLAVLRRLAHADTHAPGAAGPLKIVVAPVRAVLQPIARGLGDLTPVA
                     LAAGDERPLERVVEDLVAAAYSRTDLVERRGEFAVRGGILDVFPPTEE"
BASE COUNT          605 a         1492 c         1427 g          678 t
ORIGIN      
        1 gtagcgcaca agcaccccgt cgacggtctg gacaccgagg tgctcgcctc gatcgaccgt
       61 ccagacgacg acgtcgtggc cctgggcgcg cagcgcgagc gccacctggc gcacgtgctc
      121 ctcgaccccg ccgacgtgcg gatggaacga ggacgtgacg agagcgaccc ggtgccgacc
      181 gtctgtcatc cgcgtcctct cctggccact ggcccgtcca ccccgcgcgg gcggcgtctt
      241 gttggcatca tggcactgtg ccgctcctcg tccttgtcac cgcagccgca gcggcgccga
      301 tgggggccca ggtctatgag gagcaactcg cggcccgcgc gcagagcgcg ttgaccgcaa
      361 tcgaccccca atggcaggtg caacgacgaa tcatccgctc gatgcgctca cccttgccgg
      421 ggaccggacg gctgccgatg gggtggttgg ccggggcagg ggagcgcggt cgggctgccg
      481 tcggtcgact cgcctaccca cgccacgccg tggtccaccg aaccagcctc gaactgcccc
      541 cggctccgct cgaggtcgtc acgctgcacg atgtcgtcgc ctggcggttc gccgacgagt
      601 cggctccggt ccggtcggct gcggccgagt tgcggcgagc ggccgccgtg atttgcgtgt
      661 cggagttctc ggccggcgag gcacatgacc tgttgggagt ccgcaacccg gtcgtcgtgc
      721 ccaacggggt cgacccggcg ttcctcgacg ccgtcgcggc accgaccgag gtgctcgccg
      781 gtctggggct gaccggcccg ttcgtgttgc acgccggggg cgccgccacc cgcaagaacc
      841 tcgacgccct cgcgcaggcc tggccactcg tccgccggga gcaccccgac ctcacgcttg
      901 ccctcgccgg ccccccgcac ccgcgccgcg acgccctttt ctccggtatg ccgggtgctc
      961 gcctcctcgg ccggcttccc gacccgctcg ttcccggcct ggtggcggcg gccagcgcga
     1021 tcgtcgtgcc gtctacgtac gagggattcg ggctgccggc gttggagggc atggcggccg
     1081 gggttccggt cgtcgccgcc cggtgcgcgg ccctgcccga ggtcgtcggg gacggcggcc
     1141 tgctcgtgga cccggagccg gtcgccctcg cgcagggcct gctcgacgtg ctcgcgggtg
     1201 gccccgacgt cgccgacctc gtccgtcggg gccgcgagcg cgcgggcggg ttcacctggg
     1261 aacgcagcgc cgcggggcat gccgcggtgt gggccgcgct tggctgagga cgggttcgtc
     1321 cagcagggcg gttcggcccc ccggtccgtg cgatgtcagc cgatcaccct cgaaggccac
     1381 ggacacccgc ctagagtggg ccaatgtccg acaaccctca gccgggagag gcccacggcg
     1441 aacgacgtcg caacaggcgc ctgctcgtcg tcgtcctcct catgcttgcc gtcccagttg
     1501 tcgccgtggg cgggtatttg ggttggctta accacctcgt cacgtcgaat atcaagtttg
     1561 ccgatctcat gcctaccgga gggccgctcg tgcccgtcga cggcggggct ttcgacccgt
     1621 ctgagcctgg ggagggcggc atccccaccg ctgcggtacc cgccgagagc gctggcgaca
     1681 actacctact catcgggtcg gacgcgcgtt cgggtttggg aggtgcgcgg tccgatgtca
     1741 tcatcttcgt gcatgtaccc atcgataagc acaatgttag cctgattcac tttcctcgag
     1801 acatgtacgt gtttataccg ggacacgggc ggaacaagat caatgccgca tttgcctttg
     1861 gtggcgctcc gttgttggtc gacacggttc agggaatgct cggggtgaag atcgatcacg
     1921 tcgcgatgat cggcttcgag ggtttcaaac gcatgactga cgcggtcggc ggcttggacg
     1981 tatacgtcga agagggaggc aagatcgacg gccatgtatt tacccaaggg atgatgcatc
     2041 tcgacggtga gatggccttg gcgttcgttc gagaacgcta cgacctcagt cagggagaca
     2101 tcagtcgtgg tcggcgacag caggccttcc tgaaggccct catgctcgaa gcagtcagcg
     2161 tcgaaaccct cgccaacccg gcaaagctcg ccacattcat tgactctgca acgagcaacg
     2221 tcatcgtcga cagaggattc gacgtaggtc agatgcgtgg tgagcttctt tccttgaacg
     2281 gattgcgtcc gggcgacatc cgcttcctca cggctccgct gtccggcttc gggcgatcgc
     2341 cttacggggc gtcgatcgac ctggtcgact gggtgaaatt gacgcaattg ggcacagcca
     2401 tccgtgaaga tcggctcgac tcctactcct gagcccaggg cgaaaaggga tcagggagcc
     2461 cgatgtcagg gggtggacca cgtctttcca ccgtcgtcgg acgtgtaggt gtcgccatcg
     2521 acgatcaacc atcctgcgtt cccctcgacg ctcagggcga cgtgagtgag cagtccgtcg
     2581 ttgctgcggc caccgaggtc gacgcagccg agtttggtcg tcgcgccggc ggcgatcgag
     2641 gcgacctgca ctcccgagca gccgtctgcc acgtaggcga cgagcgcggc gagcttcgag
     2701 gcctccaccc gcgcatcgac ggcgagagcg ctcagaccgc ggctgacctc aacccagttg
     2761 cctccaccgt ccgcggtctc gacaactttg ccgctctcac acagcactgc agccgaggtc
     2821 gccgacaagg ccgagacgct cagcgccttc ccgttcccgc agggtgtgcc ggcgcggccg
     2881 ttcgaattga ccactgcggt gggtgtggcc agggatcggt accacaaggc aagggcccct
     2941 cccgacgccc aggtctgccc cgtgtcggtc gagcgcagtg ccgtttcgcg gcagcccgca
     3001 ccctcggtcc cgacgacaac caccttggcg gcatcggtga cctgcacccg cgagatcgct
     3061 ccgaagggga tggggacgtc ctcccaagtg cggccgccgt tgtcggtgtg ggccaaggag
     3121 gcgccgcccc cggggcaggc gccctgcacg aagcgccagg cccgcgccga gtcgatcggc
     3181 acgacgccga ccatgctgcc cgagccgctg aacccggtgg ccgccggcgt cgatgaggtg
     3241 gccgccgggg tggaggtgcc gccagccgag ccggggacgg tgcccgtgac agtcgccgcg
     3301 gcgccggtga ccggtgccgt cgcaggctgg cggaacgggt tacggaaggc agcgctcacc
     3361 agggcaacgt cgaccgcgag cagggccacc aaaccccacg ttgcaagtcg cgttcgcgaa
     3421 gccatcggca ccccgggaga gacgagaaga cacggagcac agtcgaagtc tggactgtgc
     3481 tgtcgtcatg ctaggtcagc ggggccgatc cggtcggcgg ttcccgcggc ttgggcgact
     3541 tcgaccaaat tcacctggcc gtagagttgt gaggctatga cctcgaccgc accctccgcc
     3601 ggcacgctgg cgcacctgcc cactgactgg gctgcccggc ataccgactg ggggctgggc
     3661 gcacacgtcg gcgaccagac cgcactgttg gacctcgcct gcgtcccggg agcccgcccg
     3721 ctcgtcctgg ccgtcctggc cggagccctt cccgtcggtg cccaggtgct cgccgtcacc
     3781 gcgacgaccc gggaggccga cgatctcgcg gccgcgctcg cggcgttcct cgacccggcg
     3841 ctcgtcgagg ttttccccag ctgggagacc cttccgcacg agcggctctc gccccgcaac
     3901 gacacggtcg gtcgtcgcct ggccgtcctg cgtcgcctcg cccacgccga cacgcacgcc
     3961 cccggcgcgg ccgggccctt gaagatcgtc gtggcccccg tgcgggccgt cctgcaaccc
     4021 atcgcccgcg ggctcggcga cctcacgccc gtggcgctcg ccgccggcga cgagcgcccg
     4081 ttggagcggg tggtcgaaga cctcgtcgcg gcggcctaca gccggaccga cctggtcgag
     4141 cggcgcggcg agttcgcggt ccgcggcggc atcctcgacg tcttcccgcc gaccgaggaa
     4201 ca
//
LOCUS       DAORPW010000207         4160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6619_length_4160_cov_782.028745, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000207 DAORPW010000000
VERSION     DAORPW010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6619_length_4160_cov_782.028745"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1198)
                     /locus_tag="PLB86_11860"
     CDS             complement(32..1198)
                     /locus_tag="PLB86_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HRC65881.1"
                     /translation="MTTTLWRTDAPTKGRPGRRWWRIAVLVAVVIGAAYLLGRGGQPG
                     SDAAYHPENPSAKGAQAVARVLAAHGVTVIVAEGQAALQRAPIDGDTTIVVSKSSDLR
                     EVTLQALATAARPAERLVLIHPERRVVRALTPGVSMAEVAHSQNALVSTCDTPDVHPG
                     ERLSRSQSQYRDTRATASCYVNDGQAVYLVTSGPGLREVVLIGSTDPITNDRTAEVEN
                     GALALRALGHSSRLVWYVPDLRDVPPTAAEQTESFTPVWWGPMLGLLAFTMLAVFWWR
                     GRRFGRLVTEPLPVVVRAVETTESRGRMYHKARDSERAGAVLRDASGRRLAAYLGLAP
                     GTPADLVAQAVSAATGQPLDQTRWLLGGPPVTTDTDLLDLAARLAALEKEIRRS"
     gene            complement(1195..1908)
                     /locus_tag="PLB86_11865"
     CDS             complement(1195..1908)
                     /locus_tag="PLB86_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HRC65882.1"
                     /translation="MSVLATASAWLTGLGQDLNPDGSEARQWLADELANDEYRDGRPL
                     LTRIIEWLVDVITDLLSGFRVTGVSGGGAPPIVVALVVVVLLAGLALLLSRLRRERRT
                     VTSSDAVLGDLNLSVVEFRDRGRAAIREGRWNDAVIDFTRAIAREAADRTLLSDAPSL
                     TAHEIGAQLAPVFPEHAEATARTMDLFDSVRYGRYAATEADARATQAHDETLRKARPV
                     LAGNSTLPVADDNIVTRPS"
     gene            complement(1905..2969)
                     /locus_tag="PLB86_11870"
     CDS             complement(1905..2969)
                     /locus_tag="PLB86_11870"
                     /inference="COORDINATES: protein motif:HMM:NF021615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HRC65883.1"
                     /translation="MSGFGPPPGPPEVPVHVRRASYLPPGGLGARPGASIAANRGRYA
                     LTRGAKPGIIPLAPLRLGDLLDGSVKHIRRNPGPVLGVSALTNAFSVLPVLVLVTAAF
                     AGSWLRATRVSTIMDAWAVGAALNLVGTIYATLVLTAVLSYAAAEAALGRRRRLGEIW
                     AAVRSRIWTVLGSQALVVLAAALPWALLVGMLSIAGQDSVWVILLVGLGFGLLAIAAN
                     AFLLPRLLYSGPAAVLEGLSLRRSIARSWSLSRGRAWSLVGVLCVVVGLAFIVYWVVE
                     LPQLGIYGLLTGFLEITGPAADSIGNFNFALANLGAAILVTPFVATSVALHYLDARIR
                     KEGFDLALQRAATADMEARS"
     gene            complement(2974..3663)
                     /locus_tag="PLB86_11875"
     CDS             complement(2974..3663)
                     /locus_tag="PLB86_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC65884.1"
                     /translation="MEQSARRARVVVLTGPSGAGKSRLAARLHAAYGWPIVRLDDFYR
                     DGDDPDLPMVTLGGHDLPDWDDPRSWNAPAAIAALEELLDTGTTRVPTYDIATSRAIG
                     HSQATAAATDLVLAEGIFAAEIVPALVERGRLAGAFCITHGRTPNAVLRFVRDLREGR
                     KPPLVLLRRGIELWRREPRIVSRAASLGASCGSPASVLKEIRTRLIASDETTRPSDRG
                     PEPWEHVRRPG"
     gene            complement(3699..>4160)
                     /locus_tag="PLB86_11880"
     CDS             complement(3699..>4160)
                     /locus_tag="PLB86_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRC65885.1"
                     /translation="VLAPQASSLLGLVSVLAPVIVSGNTAVLVASQDRPLPAVTLTEC
                     LATSDVPGGVVNVITGRTAEIAPWLASHRDVNAIDLAGATDGSNTAATADGKAVTWGD
                     LERAAADNLKRVLRPAGSGAAAVEPDWAPTPDLSRMTAFLETKTVWHPKGR"
BASE COUNT          623 a         1382 c         1599 g          556 t
ORIGIN      
        1 gcgggcggcg tggcgctcca gggctgctca gtcatgagcg acggatctcc ttctccaggg
       61 ccgccagtcg cgcggcgagg tccaacaggt cggtgtcggt ggtgaccggc ggcccaccca
      121 ggagccatcg ggtctgatcg aggggttggc cggttgccgc ggacacggct tgggcgacca
      181 ggtcggcggg ggtgccgggc gccaggccga ggtatgccgc gagccgccgc ccgctggcat
      241 cgcgcaggac cgctccggcg cgttcgctgt cgcgagcctt gtgatacatc cggccgcggc
      301 tctcggtggt ctcgaccgcg cggacgacga ccggcagggg ttcggtgacc aggcgcccga
      361 accgtcgtcc gcgccaccag aacacggcca gcatggtgaa cgccagcagc cccagcatgg
      421 ggccccacca cacgggcgtg aaggactcgg tctgctcggc cgctgtcggc ggcacgtcgc
      481 gcaggtcggg gacgtaccag accaggcggg aggagtgtcc cagcgcccgc agggccagcg
      541 ctccgttttc gacctcggcg gtgcgatcgt tggtgatcgg gtcggtgctc ccgatgagga
      601 ccacctcgcg cagcccgggg ccggacgtga cgagatagac ggcctgcccg tcgttgacgt
      661 agcaactcgc ggtcgccctg gtgtcgcggt actgcgattg cgagcggctc agccgctcac
      721 cggggtgcac gtcgggggtg tcgcacgtgc tgaccagcgc gttctgtgag tgggcgacct
      781 ccgccatgct cacgccagga gtgagggcgc gcaccacccg gcgttcggga tggatcaaca
      841 cgaggcgctc ggccgggcgg gccgcggtcg ctagcgcctg caacgtgacc tcgcgcaggt
      901 cggacgactt gctgacgacg atggtggtgt caccgtcgat cggcgcacgc tgaagcgcgg
      961 cctggccctc ggcgacgatc accgtgacgc cgtgcgcggc caagacccgg gcgaccgctt
     1021 gggcgccttt cgccgacggg ttctccgggt ggtatgccgc gtcgctgccg ggctgcccgc
     1081 cccggccgag caggtatgcc gccccgatga cgaccgcgac caggaccgcg attcgccacc
     1141 agcgtcggcc cggccggcct ttggtcggag cgtcggtgcg ccacaaggtc gttgtcacga
     1201 tggcctcgtc acgatgttgt cgtccgcgac ggggagggtg gagttcccgg ccaggaccgg
     1261 acgggccttg cgcagggtct cgtcatgcgc ctgcgttgcc cgggcgtcgg cctcggtcgc
     1321 ggcataccgg ccgtaacgga ccgagtcgaa gaggtccatc gtgcgggcgg tcgcctctgc
     1381 gtgctcgggg aagacggggg cgagttgggc gccgatctcg tgggcggtga gcgagggggc
     1441 gtcggacagc agggtgcggt cggcggcctc gcgggcgatg gctctggtga agtcgatgac
     1501 cgcgtcgttc caccggcctt cgcggatcgc ggcgcggccg cggtcgcgga actcgaccac
     1561 gctgaggttg aggtcgccga ggaccgcgtc gctgctggtc accgtgcgtc gttcgcggcg
     1621 gagtctgctg agcaacaggg ccaggccggc gaggagcacc acgaccacga gggcgacgac
     1681 gatgggcggg gccccgccac cggagacgcc agtgacccgg aagccggaca acaggtcggt
     1741 gatgacatcg acgagccact cgatgatgcg cgtcagcaac ggacgcccgt cgcgatattc
     1801 gtcgttggcc aactcgtcgg ccagccactg ccgggcttcg gagccgtcgg gattgaggtc
     1861 ctggcccagg ccggtgagcc aggctgaggc ggtggcaagg acgctcacga ccgagcctcc
     1921 atgtcggctg tggcggcgcg ctgcagcgcg aggtcgaagc cttctttgcg aatccgggcg
     1981 tccaggtagt gcagcgccac cgaggtggcg acgaacggcg tgacgaggat cgcggcgccc
     2041 aggttggcca aggcaaagtt gaaattaccg atcgagtcgg ccgccggacc ggtgatctcg
     2101 aggaagccgg tcagcaggcc gtagatgccc aactggggca actccaccac ccagtagacg
     2161 atgaaggcca gcccgacgac gacgcagagg accccgacga gggaccaggc gcgcccgcgg
     2221 gacaaggacc acgagcgggc aatggaacgt cgtagggaca gcccttccag gacggcggcc
     2281 gggccgctgt agagcaggcg tggcaggagg aaggcgttgg ccgcgatggc gagcagtccg
     2341 aacccgagcc cgacgagcag gatgacccag accgagtctt gcccggcgat cgagagcatc
     2401 ccgacaagca gggcccaggg cagggcggcc gcgaggacga cgagcgcctg gcttccgagc
     2461 accgtccaga tccgggagcg gacggcggcc cagatctcgc cgaggcgacg gcgtcgaccc
     2521 aaggcggcct cggcggcggc atacgagagc actgcggtga gcacgagcgt ggcgtagatc
     2581 gtgcccacga ggttgagcgc cgcccccacg gcccacgcgt ccatgatcgt cgacactcgg
     2641 gtcgcgcgca gccacgagcc cgcgaaggcc gcggtgacca ggacgaggac cggcaggacg
     2701 ctgaaggcgt tggtcagggc ggagacgccg agcaccgggc cggggttgcg ccggatgtgt
     2761 ttgaccgacc cgtcgagcag gtcgcccaag cgcaacgggg ccaaggggat gatccccggc
     2821 ttggcgccgc gggtgagggc atagcgaccg cgattcgcgg cgatgctcgc tcccgggcga
     2881 gcgcccaggc cgcccggggg cagataactg gctcgccgca cgtggaccgg cacctcgggc
     2941 ggaccgggcg gcgggccgaa gccgctcacc acgtcaccct gggcgacgaa cgtgctccca
     3001 cggctctggt cccctgtccg acggtcgtgt ggtttcatcc gacgctatca ggcgggtccg
     3061 gatctctttg agcaccgagg caggggaccc gcacgacgcg ccgagactcg cggcacgcga
     3121 gacgatccgc ggttcccgcc gccacaactc gatcccgcgt cggagcagga cgagcggcgg
     3181 cttgcgcccc tcccgcaggt cgcggacgaa gcgcaacacg gcgttggggg tccggccgtg
     3241 ggtgatgcag aaggcgcccg cgaggcggcc gcgttccacc aacgccggga cgatctcggc
     3301 ggcgaagatc ccctcggcga gcacgaggtc ggtggccgcg gcggtggcct ggctgtggcc
     3361 gatggcgcgg gacgtcgcga tgtcgtaggt cgggacgcgg gtcgtgccgg tgtcgaggag
     3421 ttcctcgagc gcggcgatgg ccgccggggc gttccaggat cgcgggtcgt cccagtccgg
     3481 caggtcgtgc ccgccgaggg tgaccatcgg caagtcgggg tcgtcgccgt cgcgatagaa
     3541 gtcatccagg cggacgatcg gccagccgta tgccgcgtgc agcctggccg ccagccggga
     3601 cttcccggcc cccgagggtc cggtgagcac aacgacccgg gcccgacgcg cagactgttc
     3661 cacggagcac agagcctagc ttcgggcgac ccggcggcct agcgaccctt ggggtgccag
     3721 acggtcttgg tctcgaggaa cgcggtcatc cgggacagat ccggcgtcgg tgcccagtcg
     3781 ggttcaaccg ctgctgcgcc ggacccggcc ggtcgcagga cccgcttgag gttgtcggcg
     3841 gcggctcgct ccagatcgcc ccaggtcact gccttgccgt cagcggtggc ggctgtgttg
     3901 gagccgtcag tggcgccggc caggtcgatg gcgttgacgt cccggtgcga ggccagccag
     3961 ggcgcgatct cggcggtgcg accggtgatg acgttgacga cgccgccggg cacgtcggag
     4021 gtggccaggc attcggtgag ggtcacggcg ggcagcggcc ggtcctggct ggcgacgagc
     4081 accgcggtgt tgcccgagac gatcactggg gcgagcaccg agacgagccc gagcagcgag
     4141 gacgcctgcg gggccagcac
//
LOCUS       DAORPW010000208         4127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6710_length_4127_cov_724.033644, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000208 DAORPW010000000
VERSION     DAORPW010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6710_length_4127_cov_724.033644"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLB86_11885"
     CDS             <1..285
                     /locus_tag="PLB86_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRC65886.1"
                     /translation="VEGSGSVGGGPDLRWLRAELRRVKSRGYAVNRETSEKGVTAVGR
                     AVRDESGAAVAAVSISMPSARYAATDLPRITGALAVAARAIEADLSADLG"
     gene            complement(294..1019)
                     /locus_tag="PLB86_11890"
     CDS             complement(294..1019)
                     /locus_tag="PLB86_11890"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRC65887.1"
                     /translation="MSSSEIDGQADERGACANEFGQRVGAPVDWQPATPPRPVTLAGR
                     WCTVVPLDLDHVPDLYAALAPGPPGQWTYLTFGPCDTEADLREVVSGYLANPGFVSFA
                     ILDAAGRARGIAAYLRIDPAIGSLEVGGILLGEGLRRTAAATEAMWLLARNAFEQLGF
                     RRYEWKCDALNAPSRYAAERLGFRFEGVWRQATIYKGRNRDTAWFAMTDADWPTVSAA
                     IQAWLDPATFDESGIQRRPLRVS"
     gene            complement(1026..1724)
                     /locus_tag="PLB86_11895"
     CDS             complement(1026..1724)
                     /locus_tag="PLB86_11895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRC65888.1"
                     /translation="MSERVLIPVADGELPAYLWVPESGTGPGVVLVQEIFGLSPYIHR
                     RAADLAALGYVVLAPQVFWRLGAESVPDGPGMLEEGIGLVSRLDWPAAVGDMVAAVEW
                     LRARPEIGGAGVVLVGFCFGGGLAYNVAVETPVDALVSYYGSALPGLVDILPVVEAPS
                     LHHFGEADSFIDGPTVERIRAVVGQSAACEFHTYPGADHAFDNPDFVLHHPQASALAW
                     ERTVEFLGRVLSVS"
     gene            1793..3082
                     /locus_tag="PLB86_11900"
     CDS             1793..3082
                     /locus_tag="PLB86_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC65889.1"
                     /translation="MTVRDRLRSLRVDTTPLRTSPDFRRLFFAGTVFYLGGMVSYVAL
                     PWQLYHLTGSNLAVGLLGVVELVPLVAFGLWGGALADHVDRRLMLVWTGVAQVVLTAL
                     LMLNAALPEPQTWAIYAVGAVLSAAGALQRPSREALIPRTVRHDELPAAVSVSSLGVQ
                     VGLLGGPALGGILVESVGAAAAYAIDVVGLIIATALFARLGSYPPSEDATPASLRSIA
                     EGARYAVSRRDLLGTYVIDIVAMFMAMPMVLFPALANDVLKSPHTLGLLYSAGTVGSM
                     LVTATSGWTSRVHRHGMAVVVSAAVWGAAVAAAGLTTNVPIVLLCFAIAGAADMVSAL
                     FRGVIWHQTIPDSMRGRLAGIEMLSYSVGPLGGQVRSGLVADRWSVRASITSGGVLCV
                     IGVVATAAWLRDFWSYDARTDPYAVAERQRRAADGRD"
     gene            3140..4003
                     /locus_tag="PLB86_11905"
     CDS             3140..4003
                     /locus_tag="PLB86_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRC65890.1"
                     /translation="MASDAARTTGRTERLTQFQRDGLTFDVTDSGPLDGPVVVLLHGF
                     PTDRSSWDRISPLLHAAGLRTLAPDQRGYSPGARPQGRAAYRLGEVADDVVALADAVG
                     ADAVHVVGHDWGGAIAWIVAGRHPERVASLTALTTPHPFAMTEALRSRDFDQARRSWH
                     MLAFQVPVIPDRYLASRLAAVLRPSGLPAEDATRYAARFSEPGALTAALNWYRAIPLS
                     SGVVHPCRVPTTYVWGRKDPFLGPVAARLTAHSVLADYELVELDEGHWLPERAPQACA
                     RAIIARVASVD"
BASE COUNT          568 a         1522 c         1409 g          628 t
ORIGIN      
        1 gtcgagggct caggttcggt gggcggcggc ccggatctgc gctggctgcg ggcggagctg
       61 cgccgggtca agagcagggg gtatgccgtc aaccgcgaga cctccgagaa gggcgtcacc
      121 gccgtgggcc gcgccgtccg tgacgagtcg ggcgccgctg tcgccgcggt ctcgatctcc
      181 atgcccagtg cccggtatgc cgccaccgac ctcccccgga tcacgggagc cttggcggtg
      241 gccgcccgcg ccatcgaagc cgacctgtca gccgacctcg gctgatcgac cctctagctc
      301 acgcgcagag gacggcgttg gatgccggac tcgtcaaaag tggccgggtc aagccaggcc
      361 tggatggcag cgctcaccgt cggccaatcg gcgtcggtca tcgcgaacca cgccgtgtcc
      421 cggttgcgac ccttgtaaat ggtggcctgc cgccacaccc cttcgaagcg gaaaccgagc
      481 cgttcggcgg cataccggct gggggcgttg agcgcgtcgc atttccactc gtagcgtcga
      541 aaacccaact gctcgaaggc gttccgcgcc agcaaccaca tcgcctccgt ggccgcagcc
      601 gtgcgccgca gcccctcgcc gagcaggatc ccgcccacct ccagcgagcc gatggctggg
      661 tcgatccgca ggtatgccgc gatcccccgt gcgcgtccgg ccgcgtcgag gatcgcgaag
      721 gacacgaaac cggggttggc gagatagccc gacacgacct cgcgcaggtc cgcttcggtg
      781 tcgcacggcc cgaacgtcag gtaagtccac tgcccgggcg ggccgggggc gagggcggca
      841 tacaggtcgg ggacgtggtc gaggtcgagc gggacgacgg tgcaccaccg gccggcgagg
      901 gtgaccgggc gaggcggagt ggcgggttgc cagtcgaccg gcgcaccgac gcgttgcccg
      961 aattcgttgg cgcacgcgcc ccgttcgtcg gcttggccgt caatttcgct gctggacaac
     1021 gccgttcaac tcaccgatag aacgcgcccg aggaactcga ccgtccgctc ccacgcgagc
     1081 gccgaggcct gcgggtgatg caacacgaaa tcggggttgt cgaaggcgtg gtcggcgccc
     1141 ggataggtgt ggaactcgca cgccgccgac tgccccacca ccgcgcggat ccgctcgacc
     1201 gtgggcccgt cgatgaacga gtcggcctcg ccgaagtggt gcaggctcgg cgcctcgacc
     1261 accgggagta tgtccaccag ccccggcagc gccgacccgt agtaactcac cagcgcatcg
     1321 accggagtct ccaccgcaac gttgtaggcc agcccgccgc cgaagcagaa cccgacgagg
     1381 accacgcccg cgccaccgat ttcgggccgc gcgcgcagcc attcgaccgc ggcaaccatg
     1441 tcacccaccg ctgcgggcca gtcgaggcgg ctgaccagcc caatcccctc ctcgagcatc
     1501 cccggcccgt ccggcaccga ttcggccccg agccgccaga acacctgcgg cgccagcacg
     1561 acatagccga gcgcggccag atcggcggcc cgacgatgaa tgtagggcga cagcccgaag
     1621 atctcctgaa ccaggaccac ccccggcccg gtcccgctct ccggcaccca gaggtatgcc
     1681 ggcagctcac catcagcgac ggggatcagg acgcgttcgc tcatggccgc tcagtatgcc
     1741 gcccgcgaaa ctcaggcgcg cggcatacct catctgcggc acactcactc gggtgaccgt
     1801 ccgtgaccgc ttgcgctcct tgcgcgtgga cacaaccccg ctacggacct cccccgactt
     1861 ccggcggttg ttcttcgcgg ggacggtgtt ctatctcggc gggatggtgt cgtacgtcgc
     1921 cctgccgtgg cagctctacc acctcaccgg gtccaacctc gcggtcgggt tgctcggggt
     1981 cgtcgagctc gtgccgctcg tcgcgttcgg gctgtggggc ggggcgctgg ccgaccacgt
     2041 cgaccggcgg ctcatgctcg tgtggaccgg ggtcgcccag gtcgtgctca ccgcgctgct
     2101 catgctcaac gcggccctgc ccgagccgca gacctgggcg atctacgccg tcggagcggt
     2161 gctcagcgcc gcgggcgcgc tgcaacgccc gagccgcgag gcgctcatcc ctcgcacggt
     2221 gcgccacgac gagttgcccg ccgcggtgtc tgtgtcgtct ctcggcgtgc aagtggggct
     2281 gctcggcgga ccggcgctgg gcggcatact cgtcgaatcc gtcggcgccg cagcggcata
     2341 cgcgatcgac gtcgtgggcc tcatcatcgc gaccgcgctc ttcgcgaggc tcggctccta
     2401 tccccccagc gaggacgcaa ccccggccag tctgcggtcg atcgccgagg gcgcgaggta
     2461 tgccgtgtcc cgccgcgacc tcctcggcac ctacgtcatc gacatcgtcg cgatgttcat
     2521 ggcgatgccg atggtgctct tcccggcgct ggccaacgac gtcctgaagt cgccgcacac
     2581 gctcgggctg ctctattcgg cgggcaccgt cggcagcatg ctggtcacag cgacttccgg
     2641 atggacgagc cgggtccacc ggcacgggat ggccgtcgtg gtgtccgcag cagtttgggg
     2701 cgcggcggtc gcggcggccg ggctcacgac gaacgtgcca atcgtgttgc tgtgcttcgc
     2761 gattgcgggc gccgccgaca tggtgagcgc gctgttccgg ggcgtcatct ggcaccagac
     2821 gatccccgac tcgatgcgcg ggcgactggc cggcatcgag atgctgtcct actcggtcgg
     2881 cccgctcggc gggcaggtgc gctccgggct ggtcgccgat cgctggtcgg tgcgcgcgtc
     2941 gatcacgtcg ggtggggtgc tgtgcgtgat cggcgtggtg gcgacggccg cctggctgcg
     3001 cgacttctgg tcctacgacg cccgcaccga tccgtatgcc gttgccgagc gccaacgccg
     3061 tgccgccgac ggccgcgact gacgtagttc gggtggcagg atgagtgagc accgtccggc
     3121 agcttgaagg ggtccgacca tggccagtga cgcagcccgc accaccggcc gcaccgagcg
     3181 cctgacgcag ttccagcgcg atgggctgac cttcgacgtc accgactcgg ggcctctcga
     3241 cggcccggtc gtcgtgctcc tgcacggctt ccccaccgat cgctccagct gggaccggat
     3301 ctcgccgctg ctgcatgcgg ccggcctgcg caccctggca cccgaccagc gcggctactc
     3361 gccgggagcg cggccgcagg ggcgggcggc ataccggctg ggcgaggtgg ccgacgacgt
     3421 cgtggcgctg gcggacgcgg tcggagccga cgcggtccac gtcgtcgggc acgactgggg
     3481 cggagccatc gcctggatcg tcgcggggcg ccaccccgag cgggtggcct cgctgactgc
     3541 gctgaccaca ccacacccct tcgcgatgac cgaggcgctg cggtcgcgcg acttcgacca
     3601 ggcgcgccgc tcctggcaca tgctcgcgtt ccaggtcccc gtgatccctg accgctacct
     3661 cgcgtcccgg ctcgcggcgg tccttcggcc cagcgggctg ccggcggagg atgcgacccg
     3721 gtatgccgcc cgcttctccg agccgggcgc cctcaccgcc gcgctcaact ggtaccgcgc
     3781 catccccctc tcctccggtg ttgtgcaccc ctgccgggtg ccgacgacct atgtctgggg
     3841 gcgcaaggat ccgttcctcg gtccggtggc cgcccggttg acggcgcatt cggtgctggc
     3901 cgactacgag ttggtcgagc tggatgaggg tcactggctg ccggagcgag cgccgcaggc
     3961 ctgcgctcgc gccatcatcg cccgggtcgc ctccgtcgac tgacccaccg ccctggtttc
     4021 ccccaaaatc ctcacactcg ccggcacttt ctggctcccc gagccctcgc tgccccaaga
     4081 tccccacacc cgccgatgac ctgggcgagg ggtatgccgg tcgcccc
//
LOCUS       DAORPW010000209         4100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6777_length_4100_cov_497.663535, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000209 DAORPW010000000
VERSION     DAORPW010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6777_length_4100_cov_497.663535"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLB86_11910"
     CDS             complement(<1..416)
                     /locus_tag="PLB86_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010142716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65891.1"
                     /translation="MAQHQDIHPADTHPADTHDLIRVHGARENNLKNVDVELPKRRLT
                     VFTGVSGSGKSSLVFNTIAAESQRMINETYSAFVQGFMPNLARPDVDLLDGLTTAIIV
                     DQQRLGADPRSTVGTATDALALLRILFSRLGQPHIGS"
     gene            complement(591..>1639)
                     /locus_tag="PLB86_11915"
     CDS             complement(591..>1639)
                     /locus_tag="PLB86_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65892.1"
                     /translation="TAAGGGLALGAGSVGIIAWGKAASNALGELMESRYRDAAQELAG
                     DAAHFALGAEANAQAAEQAAAASGSPEAQTAAKEARAAAEAAAKAAEAAKGAAKQAAE
                     AKTRAEVAAAYEAAKKADTEAKKKAEEAAKAREKAQSAGQTGTTNTGGTGGSGTTTTP
                     SGQSAESTCEKVRRQVWECEQGGWKSFSCQELARMLQGCRVDLTVALVDGDSSLCGLP
                     TVSTEDLVRARDEACGLLVGHPMPGVDPCTTTVEGSPEIRGSCDPTIAYGGCPDEPDV
                     VVMDDDTICFPAPPSAPPMPCVDPTPGGGPIVGETKTWSPSPSGFLQDSFGGVFVVSR
                     GGIIVIGQGYPPHP"
     assembly_gap    1640..1739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1740..2123)
                     /locus_tag="PLB86_11920"
     CDS             complement(<1740..2123)
                     /locus_tag="PLB86_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65893.1"
                     /translation="MSRLRSPIPWLAALLTLALSLFVSVPAFAAPPSLAGADGTGERT
                     WSPRVCAFTDDEIKAEEETTKTEQGTIILNDHILCALANESQPKNPVEQAIIDAFLAR
                     TGFDLDAAREHHLGAVLCSDGGVGMV"
     gene            complement(2197..3336)
                     /locus_tag="PLB86_11925"
     CDS             complement(2197..3336)
                     /locus_tag="PLB86_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRC65894.1"
                     /translation="MTDMRLSQQDARRIAVQAQLLTADRPANLFEVLDRLTLLQLEPT
                     KVVAPTHHLVLWSRLGRSYSRDELADALAAREVIEVQLHLRPAWFVALLRAEMAAWPG
                     GGAGAGAGASSPPEWQIRIAEWLKTNDACRRDILHRLDAEGAQPAGALPDTCVVPWRS
                     TGWTNDKNVQRMLDFMEQRGEIAVSHRAHGQRHWDLAERVYPDDPPVPAADARRQRDA
                     LRLHALGIARARATARQVEPDDIGEAGEPAVIEGVRGRWRVDAAYLDGLGDDTFRPRV
                     ALLSPLDRLVFDRKRMADLFDFDYKLEMYQPKATRRWGYWALPILVGDRLVGKVDATA
                     DREAGVLRVDAVHEDEPFDEPTATQIRAELASLAGWLGLDLDERF"
     gene            3476..>4100
                     /locus_tag="PLB86_11930"
     CDS             3476..>4100
                     /locus_tag="PLB86_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65895.1"
                     /translation="MEDSRGAVSLTDRVTTLQAARDVLSGLAEVLWQAQGAELAGVLT
                     LLDEVAGLAGAGRVAVTREAFTRGEVAASQAGSLTGWVAEHAPGLAVAGGGGQVAAVV
                     EAGLRRATRPVTDAACSGRVPVPVAVAVIREAERLKPQLQPEAVPTVVEAMLTMGAGH
                     GVRAVRELRARLIADYGWPGQLQADQDAAAALVSLSAPVGDELGAFSY"
BASE COUNT          529 a         1303 c         1493 g          675 t
ORIGIN      
        1 gagccgatat gcggctgccc gagccggctg aagaggatcc gcagcagcgc gagggcgtcg
       61 gtggcggtgc ccaccgtcga gcgcgggtcg gcgccgagcc gctgttggtc gacgatgatc
      121 gccgtcgtca gaccgtcgag caggtcgacg tcggggcgcg ccaggttggg catgaagccc
      181 tggacaaacg cgctgtaagt ctcgttgatc atccgctgcg actcggccgc gatcgtgttg
      241 aagaccagcg agctcttgcc cgaccccgac acccccgtga acacggtgag ccgacgcttg
      301 ggcagctcga cgtcgacgtt cttgaggttg ttctcccgcg caccatggac cctgatgagg
      361 tcgtgggtgt cggcagggtg ggtgtcagca gggtgaatgt cttggtgctg cgccatcaac
      421 ggcccttcgc tcggtgctcc cgtcaggcgc tcagactaac cgcggtgagc acacggcata
      481 ccgttgcttg gtgccggcgg ggaacctcac gctcacacgc gctcccgtcg gcgaccattg
      541 gtatgccgtg tgcccagcgc gcgactcggc gggcaccggc cttggtcacc tcacgggtgg
      601 ggtgggtacc cctgaccgat gacgatgatc ccccctcggc tcacgacgaa caccccgccg
      661 aagctgtcct ggaggaagcc gctcgggctt gggctccacg tcttggtctc gccgacgatc
      721 ggtccgccgc cgggcgtcgg gtcgacgcac ggcatcggcg gtgcgctcgg aggcgccggg
      781 aagcaaatgg tgtcgtcgtc catgaccacg acgtccggct cgtcggggca accgccgtag
      841 gcgatcgtgg gatcgcaact gccccggatc tccggcgacc cttcgacggt cgtcgtacag
      901 gggtccacgc ctggcatcgg atgcccgacg agcagcccgc acgcctcgtc gcgggcccgc
      961 accaggtcct cggttgacac ggtgggtagc ccacacaggg acgagtcgcc atcgacgagg
     1021 gccacggtca ggtcgacccg gcagccctgc aacatccgcg ccaactcctg gcacgagaag
     1081 ctcttccacc cgccttgctc gcactcccag acctggcggc ggaccttctc acaggtgctc
     1141 tccgcggact ggccgctcgg ggttgtcgtc gtgccggacc ccccggtgcc gccggtgttc
     1201 gtcgtgccgg tctggccggc actttgggcc ttctcgcggg ccttggccgc ttcctcggcc
     1261 ttcttcttgg cctcggtgtc ggccttcttg gccgcttcgt aggcagcggc aacctcggcg
     1321 cgagtcttgg cctcagcggc ttgcttggcg gcacccttgg cggcttcggc ggccttggcc
     1381 gcggcttcgg cggcagcccg ggcctccttg gctgcggtct gggcctcggg gctgcccgac
     1441 gcggcagcgg cttgttcagc ggcctgcgcg ttggcctcgg cgcctagcgc gaagtgcgcg
     1501 gcgtcgcccg ccaattcctg tgcggcgtca cggtatcggc tttccatcag ctcacccaga
     1561 gcattcgatg ccgccttccc ccaggcgatg atgccgaccg agccggcgcc gagggcgagt
     1621 ccgccacccg ccgcggtggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1741 accatgccca ccccgccgtc ggagcagagg accgctccga ggtggtgttc acgggccgca
     1801 tcgaggtcaa acccggttcg ggcgaggaag gcgtcgatga tggcctgctc gacggggttc
     1861 ttcggttggg attcgttggc gagcgcacac aggatgtggt cgttgaggat gatcgtcccc
     1921 tgctcggtct tggtcgtctc ctcttcggcc ttgatctcgt cgtcggtgaa ggcgcagact
     1981 cggggagacc acgtgcgctc gcctgtgccg tcagcgccgg ccagtgacgg cggagccgcg
     2041 aaggcgggaa cgctgacgaa caggctcaga gcaagggtga gaagggcggc gagccagggg
     2101 atgggactgc gcagacgtga catcgggtac gcctttctag ggggttaccc gcgatgagtc
     2161 gttggacccg cccctgatac acccgcaccg agacgttcag aagcgctcgt cgaggtcgag
     2221 tccgagccac cccgcgagag aggcgagctc ggcgcggatc tgcgtggccg tcggctcgtc
     2281 gaacggctcg tcctcgtgga cggcatcgac gcgcaggacc cccgcctcgc ggtccgcggt
     2341 cgcgtcgacc ttgccgacca gccggtcgcc gacgaggatc ggcagcgccc aatagcccca
     2401 ccggcgagtg gctttcggct gatacatctc gagcttgtag tcgaagtcga agaggtcggc
     2461 catccgcttg cggtcgaaga ccagccggtc gagcggggac aacaacgcca cacgtggccg
     2521 gaacgtgtcg tcacccaggc cgtcgaggta tgccgcgtcc acccgccagc gccccctcac
     2581 cccctcgatg actgcgggtt caccggcctc gccgatgtcg tcgggctcga cctgtcgggc
     2641 ggtggcccgg gctcgggcga tgccgagggc gtgcaggcga agcgcgtcgc gctggcgtcg
     2701 agcgtcagcg gctggcaccg gcgggtcgtc ggggtagacc cgctcggcga ggtcccaatg
     2761 ccgttgcccg tgcgctcggt gggagacggc gatctcgccg cgttgctcca tgaagtcgag
     2821 catgcgttgg acgttcttgt cgttggtcca gccggtcgag cgccacggga cgacgcacgt
     2881 gtcagggagg gcgccggccg gctgggctcc ctcggcgtcg agtcggtgga ggatgtcgcg
     2941 gcggcacgcg tcgttggtct tgagccactc cgcgatgcgg atctgccact cgggcgggga
     3001 cgaggcaccg gcgcctgcgc ctgcgcctcc gccgggccag gcggccatct cggcgcgcag
     3061 cagggcgacg aaccacgccg gtctcagatg cagctgcacc tcgatgactt cgcgggcggc
     3121 gagagcatca gcgagctcgt cgcgtgagta gctccggccg aggcgactcc agagcaccag
     3181 gtggtgggtc ggtgcgacga ccttggtggg ctcgagttgc aggagggtta gtcggtcgag
     3241 cacctcgaag aggttcgccg gccggtcggc ggtgagcagc tgggcctgca cggcgatccg
     3301 ccgcgcatcc tgctgcgaaa gccgcatgtc cgtcaccttc cggatgctag cccgcagccc
     3361 tgcctgaggt gtggttgatc gcggtccctg ggccccgttc gaccacacct agagggcccg
     3421 gctgcctggt ttggtggtgg ggagggctgt cggtggtcgg tcgtactgtt gttctatgga
     3481 ggacagccga ggcgccgttt cgctcaccga tcgggtgacg acgctgcagg ctgcccgaga
     3541 cgtgctgagc gggcttgctg aggtgttgtg gcaggcgcaa ggtgccgagt tggcgggagt
     3601 gttgacgctg ctggacgagg tggcagggct ggcgggggcc ggtcgggtcg ccgtgacgcg
     3661 ggaggcattc acccggggtg aggtcgctgc gagtcaggcg gggtcgttga cggggtgggt
     3721 cgcggagcat gcgccggggc tcgcggtggc tggtggtggc ggtcaggtcg cggcagtggt
     3781 cgaagccggc ctgcgccggg cgactcggcc ggtgaccgac gcggcgtgtt ccggccgggt
     3841 gcccgtgccg gtcgcggtag cggtcattcg ggaagccgag cgactcaagc cccaactcca
     3901 gccggaggcg gtgcccacgg tggtcgaggc gatgttgacg atgggcgcgg gtcacggggt
     3961 gcgggcggtg cgggagttgc gagcccggtt gatcgccgac tatgggtggc cggggcagtt
     4021 gcaggccgac caggacgcgg cggcagcgtt ggtgtcgttg tcagcgccgg tgggcgacga
     4081 gctgggggcg ttcagctacc
//
LOCUS       DAORPW010000210         4081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6829_length_4081_cov_859.506955, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000210 DAORPW010000000
VERSION     DAORPW010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6829_length_4081_cov_859.506955"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /gene="rplM"
                     /locus_tag="PLB86_11935"
     CDS             <1..281
                     /gene="rplM"
                     /locus_tag="PLB86_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRC65896.1"
                     /translation="IINAEKVALTGNKAQLKRAYRHSGFPGGLSSMSYAELLDKDPRR
                     AIEKAVRGMLPKNSLARQQLTKLKVYTGDVHPHAAQKPVPFEITQVAQ"
     gene            281..784
                     /gene="rpsI"
                     /locus_tag="PLB86_11940"
     CDS             281..784
                     /gene="rpsI"
                     /locus_tag="PLB86_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRC65897.1"
                     /translation="MADNTTDVALDVDEPELSEYTTETQNAPVGAAARKPSASAPGAA
                     TGRRKQAIARVRIVPGTGEWTINGRTLDSYFPNKVHQQLVNEPLKVLELDGAYDVLVR
                     VHGGGPSGQAGAVRLGVARSLNGVDEELNRPTLKKAGFLTRDARVPERKKAGLKKARK
                     APQYSKR"
     gene            947..2302
                     /gene="glmM"
                     /locus_tag="PLB86_11945"
     CDS             947..2302
                     /gene="glmM"
                     /locus_tag="PLB86_11945"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRC65898.1"
                     /translation="MGRLFGTDGVRGLANQDITAELALDLAVAAAHVLGDAGEFAGHR
                     PFAVVGTDGRASGEFLSAAVMAGLASAGVDVMDAGLLPTPAIAFLTADLGADLGVMLS
                     ASHNPMPDNGIKFFARGGQKLDDVVEDAIEARMQEPWTRPTGAAVGRIRRLEDGATRY
                     VRHLLTTLPHRLEGLHVVIDAAHGAASMVSPWAFREAGAFVTVIGAEPNGENINAGYG
                     STHLARLQRAVIENGAQLGIAHDGDADRCLAVDARGEVVDGDQIMCILAMAMRERGTL
                     AHDTLVATVMSNLGMLQAMTREGITVRQTGVGDRYVLEDMKAGGYSLGGEQSGHVIFL
                     DHGTTGDGVLTGLMLASRVAQTGHPLAELAAVMTRLPQVLVNVKNVDKDAVDGHPTVQ
                     SAVAEAQRELDGSGRVLLRKSGTEPLVRVMVEAADHHQAQAIADRLADVVKSQLSLSD
                     A"
     gene            complement(2359..3003)
                     /locus_tag="PLB86_11950"
     CDS             complement(2359..3003)
                     /locus_tag="PLB86_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRC65899.1"
                     /translation="MARHRPQPGTRPETPTEAWAVLSAGNARFMAGEREHPNQDVAIR
                     HRLAGKQQPFAIFFGCADSRVAAEIIFDRGLGDLFVVRTAGHTVDSSVLGSMEFGVGV
                     LGIPLIVILGHDSCGAVNAAVDAMTSGDLPGGFVRDIVERVMPSVLAASKHGEVGIDQ
                     IEAEHVRQTAALVAQRSHLIADHIAAGTLGIIGATYNLRDGQAGVVTAIGIDVT"
     gene            complement(3021..>4081)
                     /locus_tag="PLB86_11955"
     CDS             complement(3021..>4081)
                     /locus_tag="PLB86_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HRC65900.1"
                     /translation="GLMRFLDEKLRSLGTAACPPYHLAIVIGGTSAEFALKTAKYAST
                     KYLDTLPTEGSMTGHGFRDTALEEQVLELTRQFGIGAQFGGKYFCHDVRVIRLPRHGA
                     SLPVAVAISCSADRQCLGKITPEGVFIEQLEFEPGHYLPEPTAEQLAGDDAAADDVVR
                     IDLSRPMSEILAELTRQPVKTRLSLTGTLVVARDIAHAKIKERLDAGEGMPQYLKDHP
                     VYYAGPAKTPAGMASGSFGPTTAGRMDSYVDQFQAAGGSMVMLAKGNRSQQVTDACAA
                     HGGFYLGSIGGPAARLAQDCIKSVEVLEYPELGMEAVWKIEVEDFPAFVVVDDKGNDF
                     FASVTKPVAFTIEKRPGL"
BASE COUNT          635 a         1388 c         1433 g          625 t
ORIGIN      
        1 tcatcatcaa cgccgagaag gttgccctca ccggcaacaa ggcccagctc aagcgggcct
       61 accggcactc gggcttcccg ggtggtctgt cctcgatgtc ctacgccgaa ctgctcgaca
      121 aggacccccg ccgggccatc gagaaggccg tgcgcggcat gctccccaag aactccctcg
      181 ctcgccagca gctgaccaag ctcaaggtct acaccggcga cgtccacccc cacgcggcac
      241 agaagcccgt gcccttcgag atcacccagg tggcccagta atggctgaca acacgaccga
      301 cgtcgcgctc gacgtcgacg agcccgagct ctcggaatac acgaccgaga cccagaacgc
      361 cccggtcggc gcggctgccc gcaagccgtc cgcctcggct cccggtgctg ccaccggccg
      421 tcgcaagcag gccatcgccc gggtccggat cgttcccggc accggcgagt ggaccatcaa
      481 cggccgcacg ctggactcct acttccccaa caaggtccac cagcagctcg tcaacgagcc
      541 gctcaaggtc cttgagctcg acggcgccta cgacgtcctc gtgcgggttc acggcggtgg
      601 cccctccggc caggccggcg cggtccgcct cggtgtcgca cgctccctca acggtgtcga
      661 cgaggagctc aaccgcccga cgctgaagaa ggccggcttc ctcacccggg acgcccgcgt
      721 ccccgagcgc aagaaggccg gtctcaagaa ggcccgcaag gcaccgcagt acagcaagcg
      781 ttgatgtcac gactgtgacc gccgacgggc ggctcggggc aacccgggcc gcccgtcgtg
      841 cgtcttgctc acgtggggca ggagtaggtt gcttcggggt atgccgcccg cgcacctcgc
      901 gacgcggcat acgcggcata cgggattgct ggtgaaagga acactcgtgg gacgtctatt
      961 cgggaccgat ggtgtgcgcg gcctggccaa ccaggacatc acggccgagt tggccctgga
     1021 cctggcggtg gctgctgcgc acgtgctcgg tgatgccggc gagttcgcgg ggcaccgtcc
     1081 gttcgcagtc gtcgggacgg acgggcgcgc gtcgggtgag ttcctctccg ccgcggtgat
     1141 ggccggtctg gcgtctgccg gcgtcgacgt catggacgca gggctgctgc ccaccccggc
     1201 gatcgccttc ctcacggctg acctgggcgc tgacctgggg gtcatgctct cggcctcgca
     1261 caaccccatg ccggacaacg ggatcaagtt cttcgcgcgt ggcggccaga agctcgacga
     1321 cgtcgtcgag gacgccatcg aggcgcggat gcaggagcct tggacgcgcc cgaccggtgc
     1381 tgccgtgggc cggatccgtc ggctcgagga cggcgccacg aggtatgtcc gccacctgct
     1441 gacgacgctc ccgcatcgcc tggagggtct gcacgtcgtc atcgacgccg cccatggcgc
     1501 cgcctccatg gtgtcgccct gggccttccg tgaggccggg gcgttcgtca cggtcatcgg
     1561 cgcggagccc aacggcgaga acatcaacgc gggctatggg tccacccatc tggcccggtt
     1621 gcagcgggcc gtcatcgaga acggcgccca gctcgggatc gcccacgacg gcgacgccga
     1681 ccgatgcctg gcagtggacg cgcgcggcga ggtcgtcgac ggcgaccaga tcatgtgcat
     1741 cctcgcgatg gccatgcgcg agcgcggcac cctggcccac gacaccctgg tggccaccgt
     1801 gatgagcaac ctcggcatgc tgcaggccat gacccgtgag ggaatcacgg tgcggcagac
     1861 cggagtcggc gaccgttacg tcctggagga catgaaggcc ggcggctact ccctcggggg
     1921 tgagcagtcc ggccacgtca tcttcctcga ccacgggacg accggggacg gcgtcctcac
     1981 cgggctgatg ctggcctcgc gggtcgccca gaccgggcac ccgctggctg agttggcggc
     2041 cgtgatgacc cgcctgcccc aagtgctcgt caacgtcaag aacgtcgaca aggacgcggt
     2101 cgacggtcac cccacggtgc agtcggcggt ggccgaggcg cagcgggagc tcgacgggtc
     2161 gggccgggtg ttgctgcgca agtcgggcac cgagccgctc gtgcgggtca tggtcgaggc
     2221 cgccgatcac catcaggccc aggcgatcgc cgaccgcctg gccgacgtgg tcaagtcgca
     2281 gttgtcgttg agcgacgcct gaggcggctg cagcgctgac ggggcgaggc ggcatacccc
     2341 tgcggtatgc cgtgcggctc aggtgacgtc gatcccgatc gcggtgacca cacctgcttg
     2401 gccgtcgcgc aggttgtagg tcgcgccgat gatcccgagc gtgccggcgg cgatgtggtc
     2461 ggcgatgagg tgggagcgtt gcgcgaccag ggctgcggtc tggcgcacgt gctcggcctc
     2521 gatctggtcg atcccgacct cgccgtgctt gctcgcggcg agcacgctgg gcatgacgcg
     2581 ctcgacgatg tcgcgcacga aaccgccagg cagatcgcca ctggtcatgg cgtcgacggc
     2641 agcgttgacc gcgccgcacg agtcatggcc aaggatgacg atcagcggga tgccgaggac
     2701 gccgaccccg aactccatcg accccaggac gctggagtcg acggtgtggc ccgcggtgcg
     2761 gacgacgaag agatcgccta ggccgcggtc aaagatgatc tcggccgcga cccgcgagtc
     2821 ggcgcatccg aagaagatcg cgaagggctg ttgcttgccg gcgaggcggt gccggatggc
     2881 cacatcctgg ttggggtgct cgcgctcgcc agccatgaag cgggcgtttc cggcagacag
     2941 gactgcccac gcctcggtcg gtgtttccgg tcgcgtgccc ggctggggtc ggtgtcgtgc
     3001 cacaccccga gcgtatgccg ttacagcccc gggcgctttt cgatggtgaa ggcgacgggc
     3061 ttggtcacgg acgcgaagaa gtcgttgccc ttgtcatcga cgacgacgaa tgccgggaag
     3121 tcctcgacct cgatcttcca gaccgcttcc atgcccaact cggggtactc caacacctcg
     3181 acgctcttga tgcagtcctg ggccaggcgc gccgcggggc cgccgatcga gccgagatag
     3241 aagccgccgt gggcggcgca ggcatcggtg acctgctggc tgcggttgcc tttggcgagc
     3301 atgaccatcg acccaccggc cgcctggaac tgatcgacgt aggagtccat ccggccggcc
     3361 gtcgtcgggc cgaacgaccc tgaggccatg ccggccgggg tcttggccgg gcccgcgtag
     3421 tagaccgggt ggtccttgag gtactgcggc atcccctcgc cggcgtcgag acgctccttg
     3481 atcttggcgt gagcgatgtc acgggcgacg acgagcgtcc cggtgagcga caaccgggtc
     3541 ttgaccggct gccgggtgag ctcggcgagg atctcggaca tcggccggga caggtcgatg
     3601 cgcacgacat cgtcggccgc ggcgtcgtct ccggcgagtt gttcggcggt cggctcgggc
     3661 aggtagtgac cgggctcgaa ctcgagttgc tcgatgaaga cgccctcggg ggtgatcttg
     3721 cccaggcact gccggtcggc cgaacacgaa atggcgaccg ccaccggcag gctggcgccg
     3781 tgccgcggga gccggatgac gcgcacgtcg tggcagaagt acttgccgcc gaactgggcg
     3841 ccgatgccga actggcgggt caactcgagg acctgctcct caagcgcggt gtcgcggaag
     3901 ccgtgccccg tcatggaccc ttcggtcggc agcgtgtcga ggtacttcgt cgacgcgtat
     3961 ttggctgtct tgagcgcgaa ttcagcggag gtcccgccga tgacgatggc caggtggtag
     4021 ggcgggcagg ccgccgtgcc gagcgaacgc agcttctcgt cgaggaagcg catgaggccg
     4081 g
//
LOCUS       DAORPW010000211         4058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6887_length_4058_cov_473.105171, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000211 DAORPW010000000
VERSION     DAORPW010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6887_length_4058_cov_473.105171"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /gene="purH"
                     /locus_tag="PLB86_11960"
     CDS             <1..637
                     /gene="purH"
                     /locus_tag="PLB86_11960"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777843.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRC65901.1"
                     /translation="DIFTEVVVAPSFDDDALAVLTAKKNIRLLTVDFAALDAAAAGDG
                     AGRTETRPVSGGLLAQSIDRVDAVVRDESGAATGGDDPAYWTLACGEPADEATLADLA
                     FAWRAVRAVKSNAILLARDGASVGIGMGQVNRVDSCHLAVSRAGEERARGAVAASDAF
                     FPFADGPQVLLDAGVAAIVQPGGSMRDAETIAAATAAGVTMYLTGTRHFAH"
     gene            complement(653..1549)
                     /locus_tag="PLB86_11965"
     CDS             complement(653..1549)
                     /locus_tag="PLB86_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65902.1"
                     /translation="MPVLTVGTGQPTATATLTPTALPPPTPTPPPTPTLTLPGGGTTV
                     FPDRRLVGFSGYPGAPALGRLGVGNLDERGADITALAGEYAAGRRPLPVFELIATVVH
                     SRPGADGLYRTRIDEGVVRRHLEAARRHGAILLLNIQPGRAAFLDEVKHWEPWLREPD
                     VGLALDPEWAVTAGQVPGKVFGRTTGAELDAIAAYVAGIVAANRLPEKVMVYHQLSRS
                     IVREPSGLQPHPGVTLVVSVDGIGSRAQKAATWRSIVAETPDHVHKGFKLFYDEDAQI
                     GPLMTPAEVLALTPQPEYVLYE"
     gene            1814..2683
                     /locus_tag="PLB86_11970"
     CDS             1814..2683
                     /locus_tag="PLB86_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HRC65903.1"
                     /translation="MTAQILDGKATLTTVKAELAARVAALAERGIVPGLGTVLVGDDP
                     GSQWYVGAKHQDCAELGIRSIRRDLPATATQAEVEAVIDELNADPGCTGFLVQQPTGL
                     DEMALLSRVDPAKDVDGLHPMNLGWLVLGRPAPLPCTPMGVIELLRRFGVPIAGAKVC
                     VVGRGLTVGRPLGLILTRKSENATVTLCHTGTRDLAAEVRQADIVVAAAGVPNIITAD
                     MVKPGAAVLDVGVSRVTDPATGKSRVAGDVAADVAEVAGWVSPNPGGVGPMTRALLLI
                     NVVLAAERQAASA"
     gene            2680..2964
                     /locus_tag="PLB86_11975"
     CDS             2680..2964
                     /locus_tag="PLB86_11975"
                     /inference="COORDINATES: protein motif:HMM:NF022668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HRC65904.1"
                     /translation="MTTETRSALRAVRRLGLWWVVGVIAGAGLLVVGLGQVRPGGYLL
                     ATACAVGGLLRAIRPLGRAGGLAVRRRSVDVVCWLGLGATVAVAFALVRL"
     gene            complement(3031..3879)
                     /locus_tag="PLB86_11980"
     CDS             complement(3031..3879)
                     /locus_tag="PLB86_11980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC65905.1"
                     /translation="MDVLRVMWAARRSQGLVLHTVGAQAFVAGAMRRLAPRLGPDHLV
                     VSDFLCPASAQYLAAQRWALGGVDRWACIRTGDIAWLHEHFLVPSARTSFIHFPSAGS
                     TSDFGDPLPFALPASFVYASGQAHRDWDTLVAALRIAPVAAVISVPDAVANGVAATLP
                     DRVTVLPLVAPHHGRALALRASVVVVALKDTHLPAGPLVLVDALAAGAAIVATDANGS
                     RDYVVAGSTASVVPAGDAPALARAIQDLMSDPQRRSELGRNARTWANLNLRVDRFVQQ
                     VLGLAR"
BASE COUNT          601 a         1333 c         1514 g          610 t
ORIGIN      
        1 ggacatcttc accgaggtgg tcgtggcgcc gagtttcgat gacgacgcac tggccgtgct
       61 gacggccaag aagaacatcc gactgctcac cgtcgacttc gctgcgctcg atgcagcggc
      121 ggccggtgac ggggctgggc gcaccgaaac ccggccggta tcaggcggat tgctcgctca
      181 gagcattgac cgggtggacg ccgtggtgcg cgacgagtcg ggggccgcga cgggtggtga
      241 cgacccggca tactggacgc tcgcctgtgg tgagcccgcg gacgaggcga ctttggccga
      301 cctcgccttc gcctggcgcg cggtgcgtgc cgtgaagtcc aacgcgatcc tgctggcccg
      361 cgacggcgcc tcggtgggca tcgggatggg ccaggtcaac cgagtcgact cgtgccacct
      421 cgcggtctcg cgggcggggg aggagcgggc gcgcggcgcg gtggccgcgt cggacgcgtt
      481 cttccccttc gccgacgggc cgcaggtcct gctcgatgcg ggggtcgcgg cgatcgtcca
      541 gccgggtggg tcgatgcgcg acgccgagac catcgcggct gcgacggccg ccggggtgac
      601 gatgtacctc accgggaccc gccacttcgc ccactgatct cgacgagacc aatcactcgt
      661 agaggacgta ctccggctgc ggcgtgagcg cgaggacctc agccggggtc atgagcggtc
      721 cgatctgggc gtcttcgtcg tagaagagtt tgaacccctt gtggacgtga tcgggggttt
      781 cggcgacgat cgagcgccac gtcgccgcct tctgcgcccg gctcccgatc ccgtcgaccg
      841 acacgacgag ggtcaccccg ggatgcggct gcaggccgga gggctcgcgc acgatcgacc
      901 ggctgagttg gtgatagacc atgaccttct ccggcagtcg gtttgcggcg acgatccccg
      961 cgacgtatgc cgcgatcgca tcgagctccg cgccggtcgt ccggccgaac accttcccgg
     1021 ggacctgccc ggccgtgacc gcccactccg ggtcgagcgc gaggcccacg tccggctcgc
     1081 gcagccaggg ctcccagtgc ttgacctcgt cgaggaaggc cgcccggccg ggctggatgt
     1141 tgagcaggag gatggcgccg tgccgtcggg ccgcctcgag atgacgccga acgacgccct
     1201 cgtcgatccg ggtccggtag agcccgtcgg ccccgggccg gctgtggacc acagtggcga
     1261 tgagttcgaa aacggggagt gggcggcgcc cggcggcata ctcgccggcg agggcggtga
     1321 tgtcggcgcc ccgctcatcg aggttgccga ccccgaggcg gcccagggca ggcgcccccg
     1381 gataacccga gaaccccacg aggcggcgat cagggaagac cgtcgtgccg ccgccgggca
     1441 gcgtcagcgt gggggtcggt gggggtgtgg gcgtcggcgg cggcagggct gtgggagtga
     1501 gcgtcgcggt cgctgtgggc tggcccgtac cgaccgtcag gacaggcacg gagaattcgc
     1561 cccgagacgc cgcgccggtg ggaccagacg gctgttggcc tgagccgtcc ggcgctgtcg
     1621 agcacgctgt caggatgagt gcgagtgcga ctgcgggtgt gagggggaag gcgccgagcg
     1681 cagcgcggac gagcaagccg tgagcgggcg gcataccagt cggtctgcgg tgggacatgc
     1741 tgcctcggag ggtggcgggc ggtgaaccat ccacgctacg gggtggggtg cgccgacatg
     1801 gaaggattgg cgcatgacgg cgcagatcct cgacggcaaa gccacgctca ccacggtcaa
     1861 ggccgagttg gcggcacggg tggcggcgct cgccgagcgc ggaatcgttc cgggcctcgg
     1921 caccgttctt gtcggtgacg acccgggcag tcagtggtat gtcggggcca aacaccagga
     1981 ctgcgccgag ttgggcatcc gcagcatccg ccgggatttg ccggcgacgg cgacccaggc
     2041 cgaggtcgag gccgtcatcg acgagctcaa cgccgacccg gggtgcacag gcttcctcgt
     2101 ccagcagccg accgggttgg acgagatggc gctgctctcg cgggtcgacc cggccaagga
     2161 cgttgacggc ctgcacccga tgaacctggg ctggctcgtc ctgggccgtc cggcaccgct
     2221 gccgtgcacg cccatggggg tcattgagtt gctgcgtcgc ttcggagtgc cgatcgcggg
     2281 cgcgaaggtc tgcgtcgtcg gtcgtggcct gacggtcggg cgccccctgg ggctgatcct
     2341 tacccgcaag agcgagaatg caacagtcac gttgtgccat accgggactc gcgacctggc
     2401 cgccgaggtg cgtcaagctg acatcgtcgt cgcggcggcc ggggtgccca acatcatcac
     2461 cgccgacatg gtcaagcctg gcgctgcggt cctcgacgtc ggcgtgagcc gggtgactga
     2521 tcccgcgacg ggcaagtcgc gggtggccgg cgatgtggcc gcggacgtcg ccgaggtagc
     2581 cggctgggtc agccccaacc ccggtggcgt cgggccgatg actcgggccc tgttgctcat
     2641 caacgtcgtg ctcgccgcgg aacgccaagc cgccagcgca tgaccaccga aacccgctcg
     2701 gctctgcgcg ccgttcggcg cttgggactg tggtgggtcg tcggcgtcat cgccggtgcc
     2761 gggctgctcg tggtggggct cggccaggtc cggccggggg gttaccttct ggccacggcc
     2821 tgtgctgtcg gggggctgtt gcgggcgatc cgccctctgg ggcgggccgg tgggctggca
     2881 gttcgtcggc gctcggtcga cgtcgtctgc tggctcggtc tgggtgccac tgtggcggtc
     2941 gccttcgccc tcgttcgcct ctgactcgtc tggctcgacg ggccgcggga ttcgctgtcc
     3001 cgcgcggcgc tccgtcggca cgagggccgg tcagcgcgcg agcccgagaa cttgctgcac
     3061 gaaccgatca acgcgaaggt tcagattggc ccacgtccgg gcattgcggc ccagctcgga
     3121 tcggcgctgc ggatcggaca tgaggtcttg gatggcccgt gcgagagccg gcgcatcccc
     3181 ggctgggact acagacgcgg tggaacctgc gacaacgtag tcccggctgc cgttggcgtc
     3241 tgtcgcgacg atggcggctc ctgccgcgag ggcatcgacg aggaccaggg ggccagccgg
     3301 aagatgggtg tccttcagcg cgacaacgac gaccgaggca cgcagcgcca gcgctcgacc
     3361 gtggtggggt gcaaccagcg gaaggacggt cacccgatca ggcaaggtcg cagcaacacc
     3421 gttggctacg gcgtcgggaa cgctgatcac ggcggcgaca ggggcgatcc tcagggccgc
     3481 gacgagggtg tcccagtcgc gatgggcttg cccgctggcg tagacgaagg aggcgggcag
     3541 cgcaaacgga agtggatctc caaagtcgga tgtgctgccc gcagagggaa agtggatgaa
     3601 tgacgttcgc gcgctcggca ccaggaagtg ctcatgcagc cacgcgatgt cgccggttcg
     3661 aatgcaggcc caacggtcga ctccacctag cgcccaccgt tgcgctgcca ggtactgggc
     3721 actggctggg cacaggaaat cactcacgac aaggtggtcg ggcccaagcc gcggcgcgag
     3781 ccttcgcatc gcccctgcca cgaaggcttg cgcgcccacg gtgtgcaaca caagcccttg
     3841 ggatcgccgc gcggcccaca tgacgcgcag gacgtccatg ctcaggtgaa ccagtcgagt
     3901 tcgccgcccg actccccagt ctgatcgcat gcggaccaca cgaacgtctc ccgcagcggc
     3961 ctcgaaagct gagaccacga catcgggcgt ggtccacgcc acagccacga tgatgccgtc
     4021 gtggctctca gccacgtgtg gatccggacc gcgcgaag
//
LOCUS       DAORPW010000212         4043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6932_length_4043_cov_775.158475, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000212 DAORPW010000000
VERSION     DAORPW010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6932_length_4043_cov_775.158475"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /gene="mshC"
                     /locus_tag="PLB86_11985"
     CDS             complement(<1..987)
                     /gene="mshC"
                     /locus_tag="PLB86_11985"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HRC65906.1"
                     /translation="MRSWPSPFIPDIPGHGPAVRVHDTARGELVTLEPGPVAGLYVCG
                     ITPYDATHMGHAATYVTFDVLGRALRDAGHEVRYVQNVTDVDDPLLARAVRDGVDWIH
                     LADRETSLFREDMTALSVIAPDHYLGVTESIDLIVADVEALLANGMAYRIDTPDAVEA
                     DGRDVYLDISQRPGFGDLSGLTREEMLPIFGERGGDPERAGKRDPLDPLLWRAERADE
                     PSWDGATLGRGRPGWHIECTALAVRYLGMPFTVQGGGTDLVFPHHEMSAVQGCALTGT
                     RPFAQVYMHQAMVAFEGEKMSKSKGNLVLVSALRAAGVDPMVIRLALLDHHYR"
     gene            complement(1043..1882)
                     /locus_tag="PLB86_11990"
     CDS             complement(1043..1882)
                     /locus_tag="PLB86_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HRC65907.1"
                     /translation="MHGPTTAAEREAVDEALRVGTLNVVGRIVEASNVALLVEVTPPE
                     GSGPVLQAIYKPIRGERPLWDFPEGTLAGREVASALVAEATGYAVVPPTVLRDGPLGA
                     GSVQLWIGDPFATETDEGPVRIVPVGQVPDGWLGVVDGELPDGTAVTVVHEATADLRD
                     AAVLDAILNNSDRKGSHLVRDAHATLWGFDHGLTFHAEPKLRTVLWGWAGEPLRGSDI
                     ERLATFAERLADDEDALTQTLTVLLPPEDLAALGVRVRALAVGGIHPRPGGRWPSIPW
                     PAL"
     gene            complement(1896..2441)
                     /locus_tag="PLB86_11995"
     CDS             complement(1896..2441)
                     /locus_tag="PLB86_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HRC65908.1"
                     /translation="MSVFEFDPPERFVAGTVGPPGQRTFFLQARAGRRLVSVSCEKEQ
                     LGILADRLLDILDERGGAAGGDAAAAAYSDDAPLDTPIEDEFRVTTLSLGWDPDRNVV
                     LIEALRDAEPSADADPEDEDAIELPPLGVVRVTLPPAQARAFARRTRALISAGRPPCP
                     FCGGPLDPTGHVCPRANGYRR"
     gene            complement(2452..3264)
                     /locus_tag="PLB86_12000"
     CDS             complement(2452..3264)
                     /locus_tag="PLB86_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HRC65909.1"
                     /translation="MPTVLLVRHGRTTANASGVLAGWLPGVNLDEVGREQADAVGRRL
                     REAGLAPVRLVSSPLPRCVETAERILAFGEAQADADAEAGTEAGGIPLDVDERLGECR
                     YGAWTGRPLAELAKEPLWRVVQDHPSAAAFPPGEGESAGMPGESIAAMSARAVAAIRE
                     IDAEVAAAHGPEAIWVAVSHGDVIKAILADAAGTHLDLFQRIVVDPASVSVVRFTSTR
                     PFVVRTNDIGGSLAGLVPPAAAASGDAAVGGGAGADAGPGGGAVDEPVSASS"
     gene            3257..>4043
                     /locus_tag="PLB86_12005"
     CDS             3257..>4043
                     /locus_tag="PLB86_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRC65910.1"
                     /translation="MGTPSPYRHVSVCACGVLWQIGRVIVDQAMYIDGIRLAVADLSD
                     ELAALRASASGFLWIGLKDPTDEEFELVNAELGLHPLAVEDAVMGNQRAKIDAYEGSL
                     LAVLKTLRYVERTSDIETGEVMVFVGDRFVVSVRRGEANPLDHVRRRLESDPAQLRLG
                     PLAVLHAVMDSVVDNYEVIDRYIAEDLTEIEHQVFGEHTSLDSSAIYRLKREVLEFRR
                     AAYPLAEALRRFPFLNYVGKGEKKVLPFFRDVEDHLARTNDHVE"
BASE COUNT          637 a         1466 c         1376 g          564 t
ORIGIN      
        1 gcggtagtgg tgatccaaca gagccagcct gatcaccatc gggtcgacac cggccgcccg
       61 cagggccgac accagcacga ggttgccctt ggacttgctc atcttctcgc cctcgaaggc
      121 caccatcgcc tggtgcatat agacctgggc gaacggccgc gtcccggtga gtgcgcagcc
      181 ctggacggcg ctcatctcgt ggtgggggaa gacgagatcg gtcccaccac cctggacggt
      241 gaacggcata ccgagatagc ggacggccaa cgccgtgcac tcgatgtgcc agccaggtcg
      301 accgcgaccg agggtggcgc cgtcccacga cggctcgtcg gcgcgctcgg cgcgccacag
      361 caacgggtcg agcgggtcac gcttgcccgc ccgctcgggg tcgccgccac gctccccgaa
      421 gatcggcagc atctcctcgc gggtcagccc ggacaggtcg ccaaagccgg gtcgctggct
      481 gatgtcgaga tagacgtccc gcccatcggc ctcgaccgcg tcgggagtgt caatccggta
      541 ggccatgccg ttggccaaca acgcctcgac atcagcgacg atgaggtcga tgctctcagt
      601 gacgccgagg tagtggtccg gggcgatcac cgacagcgcg gtcatgtcct cgcggaacag
      661 cgaggtctcg cggtcagcca ggtggatcca gtcaacgccg tcccgcacgg cgcgcgccaa
      721 caacgggtcg tcgacgtcgg tgacgttctg gacgtaacgc acctcgtgcc ccgcatcgcg
      781 cagcgcccga cccagcacgt cgaaggtgac gtaggtggcc gcatgcccca tgtgcgtcgc
      841 gtcgtagggc gtgatgccgc agacgtagag gcccgcaacc ggccctggct ccagcgtgac
      901 gagttcaccg cgtgccgtgt cgtggacgcg gacagccggc ccgtgaccgg ggatgtcagg
      961 gatgaagggt gagggccagg agcgcaccgc atgagcctaa tcgctgcgag cgcccgcgag
     1021 gtcacctgtc ctgcggctgc gctcagaggg caggccacgg gatcgagggc cagcgcccac
     1081 caggacgcgg gtgtatgccg cccaccgcca acgcgcgcac ccggacgccc agcgcggcca
     1141 ggtcctcggg cggcagcagg accgtcagcg tctgagtcag cgcgtcttcg tcgtcggcaa
     1201 gccgctcggc gaaggtcgcg aggcgctcga tgtcgctgcc ccgcaacggt tctccggccc
     1261 aaccccacag cacggtgcgc agtttcggct cggcatggaa ggtcagcccg tgatcgaaac
     1321 cccacaaggt cgcgtgagcg tcgcggacca gatgcgaccc cttgcgatct gagttgttga
     1381 ggatggcgtc caacacggcg gcatcacgca gatcagcggt ggcctcgtgc acgaccgtga
     1441 ccgccgttcc gtcgggcaac tcgccgtcga ccacccccag ccacccgtcc ggcacctggc
     1501 ccaccgggac gatccgcacg ggcccctcgt cggtctcggt ggcgaagggg tccccaatcc
     1561 acaactgcac cgacccagcc ccgagcggtc cgtcgcgcag cacggtcggc gggacgacgg
     1621 catacccggt ggcttcggcg accagcgcgg aggcgacctc gcgaccggcg agggtgcctt
     1681 ccgggaagtc ccacagcggt cgctcgccgc ggatcggctt gtagatcgcc tgcaggaccg
     1741 gcccgctccc ctccggcggc gtgacctcca cgagcagggc gacgttggag gcctcgacga
     1801 tgcgcccgac gacgttcagt gtgccgaccc gcagggcctc gtcgacggcc tctcgctcgg
     1861 ccgcagtggt cggcccgtgc accctcgcgc gccgatcagc gccggtaacc gttggcccgc
     1921 gggcacacgt gcccggtcgg gtccagcggc ccaccgcaga acgggcacgg ggggcggccg
     1981 gcggagatca gcgcccgcgt gcgacgcgcg aacgcccgcg cttgggccgg cggcagggtc
     2041 acccgcacga cccccagcgg cggcaactcg atcgcgtcct cgtcctcagg gtcggcgtca
     2101 gcggatggtt cggcgtcgcg cagcgcctcg atgaggacga cgttgcggtc ggggtcccag
     2161 ccgagcgaca aggtggtgac gcggaattcg tcctcgatcg gcgtgtccaa gggcgcgtcg
     2221 tcgctgtatg ccgcggccgc agcatcgccg cccgccgcac cgcctcgctc gtcgaggatg
     2281 tccaggaggc ggtcggcgag aatgcccaac tgctccttct cgcacgacac gctgaccagg
     2341 cgtcgaccgg cccgagcctg caggaagaag gtccgctgcc cgggcgggcc gaccgtgcct
     2401 gcgacgaagc gctccggtgg atcgaactcg aagaccgaca tgacaaaaac cctaactgct
     2461 cgcagacacc ggctcgtcga cagcgccgcc gcctggaccc gcgtccgcac ccgcgccgcc
     2521 gcccacggcg gcatcaccgg acgctgcggc ggccgggggc acgagccccg ccagtgaccc
     2581 cccgatgtcg ttggtccgca cgacgaacgg ccgggtgctg gtgaagcgca ccaccgacac
     2641 cgacgccggg tcgacgacga tccgttggaa gagatcgagg tgggtgccgg cggcgtcggc
     2701 gaggatcgcc ttgatgacgt caccgtgcga caccgcaacc cagatcgcct ctggcccatg
     2761 ggcggccgcc acctccgcgt cgatctcccg gatcgcggcc accgcgcgag ccgacatcgc
     2821 cgcgatcgac tcacccggca taccggcgct ctccccctcg cccggcggga aggcggcggc
     2881 cgacgggtgg tcctgcacga cccgccacag cggttccttg gcgagctcag ccagcggccg
     2941 ccccgtccac gccccgtagc ggcactcccc cagccgctca tccacatcga ggggtatgcc
     3001 gcccgcttcc gtcccagcct cggcgtcagc gtccgcttga gcctcaccga aagcaaggat
     3061 ccgctcggcg gtctccacac accggggcag cggcgacgac acgagccgta cgggggccaa
     3121 cccggcctcc cgcagccgcc gaccgacggc atcggcctgc tcgcggccga cctcgtcgag
     3181 gttcacgccg ggcaaccatc cggcgagcac gccggaggcg ttggcggtcg ttcggccatg
     3241 gcggacgagc aggacggtgg gcacgccgtc accgtatcgg catgtctcgg tgtgcgcctg
     3301 cggggtgctg tggcagattg ggcgggtgat tgttgaccag gccatgtaca tcgatgggat
     3361 ccgccttgcg gtggcggatc tcagcgacga gttggccgcc ctgcgtgcga gcgcgtcggg
     3421 gttcctgtgg atcggtctga aggaccccac tgacgaggag ttcgagctcg tcaacgccga
     3481 attgggcctg caccccctcg ccgtcgagga cgccgtcatg ggtaaccagc gggccaagat
     3541 cgacgcctac gaaggcagcc tgctcgcggt cctcaagacc ctgcgctacg tcgaacgcac
     3601 ctccgacatc gagaccggcg aggtcatggt cttcgtgggc gaccggttcg tcgtctcggt
     3661 gcgacgcggc gaggccaacc cccttgacca tgtgcgtcgg cggctcgagt cagaccccgc
     3721 gcaactgcgc ctgggcccgc tggccgtcct gcacgcggtg atggacagcg tcgtggacaa
     3781 ctacgaggtc atcgaccgtt atatcgccga ggacctcacc gagatcgagc atcaggtctt
     3841 tggcgagcac accagcctgg actccagcgc gatctatcgg ctcaagcgtg aggtgctcga
     3901 gttccggcgg gcggcctacc ccttggcgga ggcgctgcgg cggttcccct tcctcaacta
     3961 cgtcggcaag ggcgagaaga aggtcctgcc cttcttccgc gacgtcgagg accacctggc
     4021 ccgcaccaac gaccacgtcg agt
//
LOCUS       DAORPW010000213         4039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6946_length_4039_cov_731.718373, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000213 DAORPW010000000
VERSION     DAORPW010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6946_length_4039_cov_731.718373"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLB86_12010"
     CDS             complement(<1..185)
                     /locus_tag="PLB86_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC65911.1"
                     /translation="MYVKSVTLKGFKSFASATTLRLEPGITCIVGPNGSGKSNVVDAL
                     AWVMGEQGAKSLRGGKM"
     gene            complement(249..1283)
                     /locus_tag="PLB86_12015"
     CDS             complement(249..1283)
                     /locus_tag="PLB86_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65912.1"
                     /translation="MRRPDVPGIRPHLPLRDAEGADRFQAFLVSAVASIAVTRAFLVL
                     TGYPQLGGGSIDGGLHLAHLLWGGLGMVIAILVTMLFISRTARTVAAVIGGVGFGLFI
                     DEVGKFVTGDNDYFFEPVAAIIYATFVVIVLIVRLVVSRTPLTPREQLINAVELLKDS
                     AAHDLDDVERARLVALLRAADPADLLVAPLLALVDGLPPQRPSRSWIAQGYAAARRLV
                     MSAPRLEVVKRTSVWAFTAFCGLALADALWAAGHDPSLQNRVYVAVAAGSALVAVVAL
                     VGWLRGPRGRALQLFEVALLAQVLGVQFFRLLAASFQGYLTAFVSLALIGLCRAMLFE
                     HRTRHAHDGG"
     gene            complement(1276..1590)
                     /locus_tag="PLB86_12020"
     CDS             complement(1276..1590)
                     /locus_tag="PLB86_12020"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRC65913.1"
                     /translation="MSTTPEIPAELTRLRASIDNLDAALVHLLAERFRCTQQVGVLKA
                     TVGLPPSDPAREEQQMSRLRELAAEAGLDPVFAEKFLGFIIAEVIRNHGTIAQRATIP
                     DA"
     gene            complement(1692..1979)
                     /locus_tag="PLB86_12025"
     CDS             complement(1692..1979)
                     /locus_tag="PLB86_12025"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRC65914.1"
                     /translation="MPDERVTVFVRGHVQGVGFRWWARARALELGLVGYARNLDDGRV
                     EVLAQGPTEAIDAMVRLLSEQPSSVRRPGRVAGVVAQYAQPIAGLVGFKER"
     gene            complement(2001..3932)
                     /locus_tag="PLB86_12030"
     CDS             complement(2001..3932)
                     /locus_tag="PLB86_12030"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRC65915.1"
                     /translation="MTAAQASAPLALPTAASAHDWLSDRAATGLAKARSLVADLKAAP
                     PADALATLKAWNDVDQALGSVSSPASLFAEVHPDEAVRERADVAGQEVQKLATELGLD
                     RELFEVFAALDPAGLDADAARFLDKTLRDFRRSGVDRDEPTRERIRAINERLTVLGQE
                     FSKNIRNGVRSIEVTPDRLEGMPQDWLDAHPADERGLVTVTTDYPDVVPLRTFCADAQ
                     ARRDLTMEFLTVGWPANDALLHELFDLRHELAQLLGYETWADYDAEVKMIKEGGAIPV
                     FIEKIADAALESGVRDREVVLARMRADVPDATAIDSADLAYYANLVSKEQLEVDAQLV
                     RTYLDFTKVRAGLLEVTGRLFGLRYEPAADAVVWDEAVAAYDVYRADGDEPLGRIYLD
                     LHPREGKYKHAAQFDLVTGVSGRQLPEGVLVCNFSRGLMEHDHVVTLFHEFGHLVHHV
                     LAGQQEWFRFSGVATEWDFVEAPSQMLEEWAWDADILQTFATNEAGEPIPADLVRRMR
                     RADDFGKGYLARTQMFYAALSYWFHTERPADLTARTRELQAQYSLFPYIEGTHFTCAF
                     GHLEGYSSAYYTYMWSLVIAKDLFSAFDRSNMFDAEVAGRYRDRVLAPGGSKDAADLV
                     ADFLGRPYTFDAYAAWLAE"
     gene            complement(3943..>4039)
                     /locus_tag="PLB86_12035"
     CDS             complement(3943..>4039)
                     /locus_tag="PLB86_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HRC65916.1"
                     /translation="PRCGTPIRREAFMNRSSFSCPSCQRRPRIVN"
BASE COUNT          655 a         1341 c         1475 g          568 t
ORIGIN      
        1 tccatcttgc cgccgcgcag gctcttggcg ccctgctccc ccatgaccca ggcgagggcg
       61 tcgacgacgt tggacttgcc cgacccgttg ggaccgacga tgcaggtgat gcctggctcg
      121 aggcgcaagg tcgtcgcgga ggcgaacgac ttgaacccct tgagggtcac gctcttgacg
      181 tacaaggtga cggtggcttc ctgctactgg tctggcgagg tcggatctgg cgaatgatcc
      241 cgcccaggct acccgccgtc gtgggcatgc cgggtccggt gctcgaagag catcgctcgg
      301 cacagcccga tgagggccag cgacacgaag gccgtgaggt agccctggaa ggaggcagcg
      361 agcagccgga agaactgcac gccaaggacc tgcgccaaga gcgcgacctc gaacagctgc
      421 agggcgcggc cccggggccc gcggagccac ccgacgaggg ccaccacagc cacgagcgct
      481 gagccggcgg ccacggcgac gtagacgcgg ttctgcagcg atgggtcgtg cccggccgcc
      541 cacagcgcat cggccagcgc gagaccgcaa aaggcggtga aggcccacac cgaggtccgc
      601 ttgaccactt ccaggcgtgg cgcggacatg accaggcgcc gcgcggcggc atacccctgg
      661 gcgatccagg accgcgacgg gcgctgaggg ggcaggccgt caaccagcgc cagcagtggc
      721 gcgaccagca ggtcggcggg atccgcggcg cgcagcaggg cgaccaaccg ggcccgctcg
      781 acgtcgtcga ggtcgtgagc ggccgagtcc ttgagcagtt ccacggcgtt gatgagttgt
      841 tcgcggggcg tgagcggggt ccggctgacg acgaggcgca cgatgaggac gatgacgacg
      901 aacgtcgcat agatgatcgc cgcgaccggc tcgaagaagt agtcgttgtc ccccgtgacg
      961 aacttgccga cctcatcgat gaacaacccg aacccgaccc caccgatgac ggcagccacc
     1021 gtgcgcgccg tgcggctgat gaacagcatc gtcacgagga tggcgatcac catgccgagg
     1081 ccaccccaca acaggtgggc gaggtgcaga ccgccgtcga tcgacccgcc gcccaactgc
     1141 gggtagcccg tgaggacgag gaaggcgcgg gtcacggcga tcgacgcgac cgcgctgacg
     1201 aggaacgcct ggaaccggtc ggctccctcg gcgtcccgca gcggcaggtg cggccggatt
     1261 cccggcacgt cgggcctacg catcaggaat ggtggcccgc tgagcgatcg tgccgtggtt
     1321 gcgaatgacc tcggcgatga tgaaacccag gaacttctcg gcgaagaccg ggtccaggcc
     1381 cgcctcggcc gcgagctcgc gcagccgcga catctgctgc tcctcgcgag ccgggtcgga
     1441 cggcggcagc cccacggtcg ccttgaggac ccccacctgc tgcgtgcagc ggaagcgctc
     1501 agccagcagg tggaccaggg ccgcgtcgag gttgtcgatg ctcgcgcgca gtcgggtcag
     1561 ctcggccggg atctcggggg tggtgctcac caggtgaggg tatgccgtgt ggtcgcctcc
     1621 gcgggatccg gtgcccacgt gctgggcgcc agcggccggg ccgccgcggc gcgccgggac
     1681 ggcataccgg gtcagcgttc cttgaaaccc acgagaccgg cgatgggctg ggcgtattgc
     1741 gcgaccacac cggcgacccg accgggtcgt cggacggacg acggctgctc cgacagcagg
     1801 cgcaccatcg cgtcgatcgc ctcggttggc ccctgcgcga gcacttcgac ccggccgtcg
     1861 tcgaggttgc gggcgtagcc gaccagtccg agttccagcg cgcgggcgcg cgcccaccag
     1921 cggaacccga ccccttggac gtgcccccgc acgaaaaccg tcacccgctc gtcaggcatg
     1981 actgctcacg tgcctggctg ctactccgcc agccaggcgg catacgcgtc gaaggtgtag
     2041 gggcgaccga ggaagtcggc gaccaggtcg gcggcgtcct tgctgccccc gggggcgagc
     2101 acgcggtcgc ggtaacggcc ggccacctcc gcgtcgaaca tgttcgagcg gtcgaaggcc
     2161 gagaacaggt ccttggcgat gaccaggctc cacatatagg tgtagtaggc gctggagtag
     2221 ccctccaggt gcccgaacgc gcaggtgaag tgggtgccct cgatgtaggg gaagagcgag
     2281 tactgcgcct gcaactcacg cgtgcgcgcg gtcaggtcgg ctgggcgttc ggtgtggaac
     2341 cagtaggaca gcgcggcata gaacatctgg gtgcgcgcga ggtagccctt gccgaagtcg
     2401 tcggcccgac gcatccgccg gacgaggtcg gccgggatcg gctcgccggc ctcgttggtg
     2461 gcgaaggtct gcaggatgtc ggcgtcccag gcccactcct cgagcatctg gctgggtgcc
     2521 tcgacgaagt cccactcggt cgcgacgccg gagaaccgga accactcctg ctggccggcg
     2581 agcacgtggt ggacgaggtg accgaactcg tggaagaggg tgacgacgtg gtcgtgctcc
     2641 atcagcccgc gggagaagtt gcagacgagg acgccctcgg ggagttggcg gccggagacc
     2701 ccggtgacca ggtcgaactg agcggcgtgc ttgtacttcc cctcgcgcgg gtgcaggtcg
     2761 aggtagatcc gcccgagcgg ctcgtccccg tcggcgcgat agacgtcgta tgccgcgacc
     2821 gcctcgtccc agacgacggc gtcggcggcc ggctcatagc gcaggccgaa gaggcgcccg
     2881 gtcacctcca agagaccggc ccggaccttg gtgaagtcga ggtaggtccg caccaactgg
     2941 gcgtcgacct ccaactgctc cttgctgacg aggttggcgt agtaggcgag gtcggccgag
     3001 tcgatcgcgg tggcatccgg gacgtcggcg cgcatccggg ccaggacgac ctctcggtca
     3061 cgcacaccgg actcgagggc ggcgtcggcg atcttctcga tgaagacggg gatcgccccg
     3121 ccctctttga tcatcttgac ctcggcgtcg tagtcggccc acgtctcgta gccgagcaac
     3181 tgggccaact cgtggcgcag gtcgaacagc tcgtgcagca gtgcgtcatt ggccggccag
     3241 ccgacggtga ggaactccat cgtcaggtca cgtcgggctt gggcgtcggc gcagaacgtg
     3301 cgcagcggca ccacgtcggg gtagtcggtc gtcaccgtga ccagaccccg ctcgtcggcg
     3361 gggtgggcgt cgagccagtc ctgcggcata ccctccaacc ggtccggggt gacctcgatc
     3421 gagcggaccc cgttgcggat gttcttggag aactcctggc cgagcaccgt gagccgctcg
     3481 ttgatcgcac ggatccgctc gcgggtcggc tcgtcgcggt cgacaccgga gcggcggaag
     3541 tcgcgcagcg tcttgtcgag gaaccgggcc gcgtcggcgt ccaggccagc cgggtcgagc
     3601 gcggcgaaga cctcgaacag ctcacggtcc aggccgagct cggtggcgag tttctggacc
     3661 tcctggccgg ccacgtcggc ccgctcgcga actgcctcat cggggtggac ctcggcgaag
     3721 agggacgcgg gtgaggagac cgaccccagg gcctgatcga cgtcgttcca tgccttcaag
     3781 gtcgccagtg cgtctgcggg cggggccgcc ttgaggtcag cgacgaggct gcgggccttc
     3841 gcaaggccgg ttgcggcccg atcggagagc cagtcgtggg ccgaagcggc ggtgggcagg
     3901 gcaaggggcg cggatgcctg agcagcggtc acccgcgcga ctctagttca cgatgcgggg
     3961 gcgccgctgg cagctcggac agctgaagga cgagcggttc atgaaggcct cccgccggat
     4021 cggcgtcccg caccggggg
//
LOCUS       DAORPW010000214         4027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_6991_length_4027_cov_675.775680, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000214 DAORPW010000000
VERSION     DAORPW010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6991_length_4027_cov_675.775680"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /gene="ffh"
                     /locus_tag="PLB86_12040"
     CDS             complement(<1..1133)
                     /gene="ffh"
                     /locus_tag="PLB86_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRC65917.1"
                     /translation="MFNNLSDRLTATFRNLRGKGRLTEHDINATLRDIRMALLDADVA
                     LPVVKAFVAAIRERALGAEVSQALNPGQQVVKIVNEELVTILGGQTRPLNLAKNPPTV
                     IMLAGLQGSGKTTLAGKLGYWLKEQGHTPMLVAADLQRPNAVTQLEVVGQRAGVPVYA
                     PERGNVAGHDAVGGAGEGTRSYGDPVKVADDGIQYAIARQHDVVIVDTAGRLAVDADL
                     MAQAKDIRAAISPDEVLFVIDAMIGQAAVETAQAFQEGVGFTGVVLSKLDGDARGGAA
                     LSIAHVTGRPILFSSIGEGVKDFEVFHPDRMASRILDLGDVLTLIEQAERAFDKQHAD
                     EMARKFLAEEDFTFDDFLEQMAAIKRMGNFKSMLGMLPGMQQMR"
     gene            complement(1220..3619)
                     /locus_tag="PLB86_12045"
     CDS             complement(1220..3619)
                     /locus_tag="PLB86_12045"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRC65918.1"
                     /translation="MTDVSQRRLDVAGTRSFAAAGAGPARRAALAEFARTWLVDRWAD
                     AAQGRPTPGLALAAVGSIARGDAGPLSDYDLVLLHEGRAMSQKDIDRFADRLWYPMWD
                     SGIKIDHSVRTLGQCRQVAGADLSAAVGLLDLSHLAGEENLVAAARSAVAQDWRANAR
                     RRLPEVLDAVTARHTRMGDCAHLIEPDLKEARGGLRDMTVLRAMTAAWLADRPHGEVD
                     AAYLRILDVRDAVHMVTGRARDRLGREDHDAVAALLGYDDTDLMLTDLSQAARIVGYA
                     LDGTLRRASQSQRARVLRVGPRRPSLEALGYGLFLHDGEAVLGPGVDPHANPTLALRA
                     AGIAARAGVSLAPATLANLAAGCPPLPQPWPADALALFTDLLAAGPGLVGVWEGLDLA
                     GIIEQWIPEWREVRSRPQHNAVHRHTVDRHSIEAVVVAGGLVRDVQRPDLLLLAALLH
                     DIGKVAGAADHAEVGAPVADRILGRLGVADADRALVVRLVREHLTLVELATRRDPHDP
                     ATVASLSAAVDGSLETLALLRALTEADACAAGPAAWTDWRAQLVARLTAVGARALRER
                     PERSDAEAVGGISDGGLGDDGPGATAAEIAEVAAGRPVVRVDRLGTAHRLHIVDNDRT
                     GLFADTTGLLAATGFIVRSALLRTVDGIAVNEWHVDSPSGDAPDGGMLSRGLGRLAMG
                     DRSPLAPLARRRRALEVTAHASLGRPGMTRAMVLPEASRQSTVIEVRATDRRGLLHDL
                     GSAITDAGLSVRSAHIATYAGQALDTFYLTRADGSPLAPAEVGRTIALLIDTSDGRAA
                     S"
     gene            complement(3675..4013)
                     /locus_tag="PLB86_12050"
     CDS             complement(3675..4013)
                     /locus_tag="PLB86_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRC65919.1"
                     /translation="MKLVTAIIKPHQLDTVKEALEAYGISGMTISEASGFGRQKGHSE
                     VYRGAEYTVDFVPKVRLEVLSDDVDTAGVIDVILKAAQTGRIGDGKIWSVPVHDLVRV
                     RTGERGVDAI"
BASE COUNT          585 a         1414 c         1444 g          584 t
ORIGIN      
        1 cgcatctgct gcatccccgg cagcatcccg agcatcgact tgaagttgcc catccgcttg
       61 atcgcggcca tctgctcgag gaagtcgtcg aaggtgaagt cctcttcggc gaggaacttg
      121 cgggccatct cgtcggcgtg ctgcttgtcg aacgcccgct cggcctgctc gatgagggtg
      181 aggacgtcac ccaagtcgag gatccgcgac gccatccggt cggggtggaa gacctcgaag
      241 tccttgaccc cctcgccgat cgaggagaac aggatcgggc ggccagtgac gtgcgcgatc
      301 gagagcgcgg caccaccgcg agcgtcgccg tcgagcttgg acagcacgac gccggtgaag
      361 ccgacgccct cttggaaggc ctgcgcggtc tcgaccgccg cctgaccgat catcgcgtcg
      421 atgacgaaga ggacctcgtc gggactgatc gccgcccgga tgtccttggc ctgcgccatg
      481 aggtcggcgt cgaccgcgag ccggccagcg gtgtcgacaa tgacgacgtc gtgctgccgg
      541 gcgatggcgt actggatgcc gtcgtcggcg accttgaccg ggtcgccgta ggaccgggtg
      601 ccctcaccgg cgccgccgac cgcgtcgtgc ccggcgacgt tgccgcgctc aggggcatag
      661 accggcactc cggcgcgctg cccgaccact tcgagctggg tgacggcatt gggccgctgc
      721 aggtcggcgg ccacgagcat cggcgtgtgc ccctgctcct tgagccagta gcccagtttg
      781 cctgcgaggg tggtctttcc agacccttgg agaccggcca gcatgatgac cgtgggcggg
      841 ttcttggcca gattgagcgg gcgggtctgc ccgccgagga tggtgacgag ctcctcgttg
      901 acgatcttga cgacctgctg gcccgggttg agggcctgac tcacctcggc gcccagcgct
      961 cgctcgcgga tcgccgcgac gaacgccttg acgacgggca gggcgacatc ggcgtcgagc
     1021 agcgccatcc ggatgtcgcg cagggtcgcg ttgatgtcgt gctcggtgag gcgccccttg
     1081 ccgcgcaggt tgcggaaggt cgcggtcagc cggtcggaca ggttgttgaa caccccagaa
     1141 ccttaccttt cgcgcggcat acgagcctgc cggtatgccg cccgcgcccc ttcgcgagaa
     1201 cgttcggctc aggcgcccgt caactcgcgg cccgaccgtc gctggtgtcg atgagcaggg
     1261 cgatggtgcg ccccacctcc gcaggcgcca gcggtgagcc gtcggcgcgg gtgaggtaga
     1321 aggtgtccag cgcctgaccg gcatacgtcg cgatgtgggc gctgcggacg gagagcccgg
     1381 cgtcggtgat cgcggacccg aggtcgtgca ggagcccgcg gcggtcggtg gcgcgcacct
     1441 cgatgaccgt cgactggcgg gacgcttcgg gcagcaccat ggcccgcgtc atcccgggtc
     1501 ggccgagcga cgcatgcgct gtcacctcaa gggcccgccg ccgtcgagcc agtggcgcca
     1561 acggtgatcg gtcgcccatc gccaagcgac ccaacccgcg ggagagcatg cccccatccg
     1621 gggcatcccc cgacggagag tcgacgtgcc actcgttgac ggcgatgccg tcgacggtcc
     1681 gcagcagcgc cgaacgcacg atgaagccgg tggccgcgag cagccccgtg gtgtcggcga
     1741 acaggcccgt ccggtcgttg tcgacgatgt gcaggcggtg cgctgtgccg agccggtcga
     1801 cccgcacgac cggtcgcccg gcggccacct cagcaatctc ggcagccgtg gcacccgggc
     1861 cgtcgtcacc cagcccgcca tcgctgatcc cgccgaccgc ctccgcatcg gaccgctcgg
     1921 gccgctcgcg cagggcccgc gcgccgaccg cggtgagccg ggcgacgagt tgagcccgcc
     1981 aatccgtcca cgctgccgga cccgccgcgc aggcgtcggc ctcggtgagg gctcgcaaca
     2041 gcgccagcgt ctccaacgag ccgtcgacgg ccgccgaaag ggaggcgacg gtcgccgggt
     2101 cgtgcgggtc gcgccgggtg gccaactcga cgagggtcag gtgttcgcgc acgagccgca
     2161 caacgagcgc ccgatcggcg tccgcaaccc ccagtcgacc gaggatccga tcggcgaccg
     2221 gggcaccgac ctccgcgtga tcggcggcgc cggcgacctt gccgatgtcg tgcagcagcg
     2281 cggccagcag caagaggtcg gggcgctgga catcgcgcac caacccgccg gccacgacga
     2341 ccgcctcgat cgagtgtcgg tcgacggtgt gccggtggac cgcgttgtgc tgcggccggc
     2401 tgcggacctc gcgccattcc gggatccact gctcgatgat gccggccagg tccaagccct
     2461 cccagacccc gaccaggccc ggaccggcag cgagcaggtc ggtgaagaga gccagcgcgt
     2521 cagccggcca gggctgcggc agcggcgggc agcccgccgc gaggttcgcc agggtggccg
     2581 gggccagtga cacccctgca cgtgcagcga tcccggccgc ccgcagcgcc aacgtggggt
     2641 tggcgtgggg gtcgacgccc ggcccgagga ccgcttcgcc gtcgtgcagg aacaggccgt
     2701 aaccgagggc ttccaggctc gggcgacgcg gcccgacccg cagcacccgg gcccgttggg
     2761 attgcgaggc gcgccggagc gttccgtcga gcgcgtagcc cacgatgcgc gcagcctgcg
     2821 acaggtcagt gagcatgagg tcggtgtcgt cataaccgag cagggccgcg acggcgtcgt
     2881 ggtcctcgcg accgagtcgg tcgcgggccc ggccggtgac catgtggacg gcatcgcgca
     2941 cgtcgaggat gcgcaggtat gccgcgtcga cctcaccgtg cggacggtcg gcgagccatg
     3001 ctgccgtcat ggccctcagg acggtcatgt cgcgcagtcc gccgcgagcc tccttgaggt
     3061 ccggctcgat gaggtgggcg cagtccccca tccgggtatg ccgtgcagtc accgcgtcga
     3121 gcacctccgg caggcgccgc cgggcattgg cgcgccagtc ctgggcgacc gccgagcggg
     3181 ccgcggcgac gaggttctcc tcccccgcaa ggtggctgag gtcgagcagt ccgaccgccg
     3241 cggacaggtc ggcgccggcc acctggcggc actggcccag ggtccggacc gaatggtcga
     3301 tcttgatccc ggagtcccac atgggatacc agagccggtc ggcgaagcgg tcgatgtcct
     3361 tctggctcat tgcccggccc tcatggagca ggacgaggtc gtaatcactc agtggtccgg
     3421 cgtcgccccg ggcgatgctt cccacagccg ctagggccag gcccggcgtc gggcggccct
     3481 gcgcggcgtc ggcccagcga tccaccagcc aggtgcgggc gaattcggcg agcgcggccc
     3541 gacgcgccgg accggcaccc gcagcagcga agctgcgggt gccggccacg tcgagccgtc
     3601 gttgcgaaac gtcggtcatg ggatccgaac ttccttgtcg tcagtgctcg gacaccaccg
     3661 gcgtgcaggt gacgtcaaat ggcgtcgacg ccgcgctcgc cggtgcggac acgcaccaga
     3721 tcatgcacgg gaaccgacca gatcttgcca tccccgatcc gtccggtctg ggcggccttg
     3781 aggatgacgt cgatgacccc tgcggtgtcg acgtcgtcgg acagaacctc cagacgcacc
     3841 ttcggcacga agtcgacggt gtactcggcg cctcggtaga cctcgctgtg gcccttctgg
     3901 cggccgaagc cagaggcctc gctgatcgtc atgcccgaga tgccgtaggc ctcgagggcc
     3961 tccttgacgg tgtcgagctg gtggggcttg atgatggcgg tgacgagctt catgcgctcg
     4021 ctccttc
//
LOCUS       DAORPW010000215         4019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7014_length_4019_cov_874.438951, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000215 DAORPW010000000
VERSION     DAORPW010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7014_length_4019_cov_874.438951"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1147
                     /locus_tag="PLB86_12055"
     CDS             206..1147
                     /locus_tag="PLB86_12055"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRC65920.1"
                     /translation="MRSLLPALRPRPLRARRVPMLLVALLFGALVMPSAAASPVPAAG
                     TSLNTVKAVIAPGTYGGQIPATVTFNGSGSYCVELCSIASWSWNFGDGATGTGAVASH
                     TYTTSGNFTVTLTVVSNEGVTDTVTTQLMTVQQTIASFTMTPSAGVLPVTASFDASSS
                     SSNWDRPIVSYAWDFGDGTTGTGRLTTHTYTTPGVRYITLTVTDSGGTIKSTSGAITV
                     QDPLLAPNNLRATSPAKGITSLTWTNRTVMVTNLTVERCTGSTCTNFVAVGSVSGAYT
                     SFGESGLRSGTTFRYRVRATSYLGTSVVSSSVSVKVR"
     assembly_gap    1158..1257
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1258..1548
                     /locus_tag="PLB86_12060"
     CDS             <1258..1548
                     /locus_tag="PLB86_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65921.1"
                     /translation="VQDPLLASTNLTATSPSKGVTSLTWTNRTVMISTLQVERCTGSR
                     CTNFVPVSSVPGTFTSFSESGLRSSTVFRYRIRATDYLGNTAVSSIVSVKTR"
     gene            1635..2603
                     /locus_tag="PLB86_12065"
     CDS             1635..2603
                     /locus_tag="PLB86_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRC65922.1"
                     /translation="METAQQVCRVAYLNLRDAQRVMSLEDEQALRREAMAWLTVRTND
                     GRDAISREDLTQFRFRGERLTLIDQGRGIRKPAACRAALSIMTVHTPPGRERPYNDMR
                     GPDGLPRYKMRADSRSGADNRAVLQALELHLPVIWLVGVAPGWFQVVFPVFVIGAEPE
                     LDQFVLAYDAAQSLPLLGSPMEEVLRHYVTRETRQRVHQPVFRSMVMLAYQTQCAVCS
                     LRHSQLLDAAHIIPDSDEGGIAAVRNGLALCKIHHAAYDAGILGISPDYHVAIRDDVL
                     AEVDGPLFEHGIKGLHGQPLMVTPRRRADRPDPELLQQRYADFLSA"
     gene            complement(2715..3659)
                     /locus_tag="PLB86_12070"
     CDS             complement(2715..3659)
                     /locus_tag="PLB86_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HRC65923.1"
                     /translation="MTTDEHTTASFEGRPLPRPDEAPFDQGLAFDLTTLINRRNALTL
                     FGLGAAGTALAACSSGTSTGSTTSAAATTSAKSSSSSSSSSSSSSSGTSALTEIPDET
                     AGPYPGDGSNGPDVLAQSGVVRKDITASFGTSTTKAEGVPMTLELTILDIAGGGGAMK
                     GAAVYAWHCDRAGQYSMYSSGITNENYLRGVQITDANGVVSFTSIFPGCYSGRWPHIH
                     FEVYPDQASISDSTKAIATSQVALPKTACDAVYATTGYESSVGNLAKLSLTSDNVFGN
                     DGGVHQLGASTGDAASGYAVRLTVPVDTRTAPTGGSRP"
     gene            3768..>4019
                     /locus_tag="PLB86_12075"
     CDS             3768..>4019
                     /locus_tag="PLB86_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRC65924.1"
                     /translation="MPYPDIAFCRLCGSPSDRRVPQGEDRERSVCRACGYVDYVNPTN
                     VVGTVPTWGPDGDHVLLCLRAIEPRKGFWTLPAGFLEYGE"
BASE COUNT          607 a         1318 c         1321 g          673 t
ORIGIN      
        1 acgcagtgcg tgcctgaggt acgcggctct tccaggggtc ccggcgccct atcggccagt
       61 ccggcagggc ggcataccgg acttttagga cacaggcaac tgccccaagg tccaatagcg
      121 cgcgaacccc gttgtcccta gcgtcggcat ggtcggggca ttctcttgcg ctcggcgacg
      181 acctgttcga gaggattttc cgcccatgcg ctcactgctt cccgccctgc ggccccgacc
      241 actacgcgcc cgccgcgtgc ccatgctcct tgtcgccctc ctcttcggcg cgctggtcat
      301 gcccagcgct gccgccagcc cggtccccgc ggccgggaca tcgttgaaca ccgtgaaggc
      361 ggtcatcgcg ccgggaacgt acggtggaca aattccagcg acggtgacct tcaacgggtc
      421 tggctcctac tgcgtggagt tgtgctcgat tgcgtcgtgg tcgtggaact tcggtgacgg
      481 cgcgaccggg accggcgctg tggcgagtca cacctacacc acgagcggca acttcaccgt
      541 gactctgacg gtggtgtcga acgaaggggt gaccgacacc gtgacgacac aactcatgac
      601 cgtccagcag acgatcgcaa gcttcacgat gaccccgtcg gcgggagttt tgccggtgac
      661 tgcgagcttc gacgccagct cctccagctc caactgggac cgcccgatcg tcagctacgc
      721 gtgggacttc ggtgacggca cgaccgggac cggccgcctg accactcaca cctacacgac
      781 tcccggggtg cgctacatca ccctcacggt gaccgacagc ggcggcacca tcaagtccac
      841 gtccggcgcg atcaccgtcc aggacccgct gctggcgccg aacaacctcc gggccacgtc
      901 cccggccaag gggatcacct cgctgacctg gaccaaccgc acggtcatgg tgaccaacct
      961 gacggtcgag cgctgcaccg gctcgacgtg cacgaacttc gtcgcagtcg ggtcggtgtc
     1021 gggggcgtac acgagcttcg gcgagtcggg cctgcggtct gggacgacgt tccgttaccg
     1081 ggtccgggcg accagctacc tgggcacctc ggtggtctcc tcaagcgtgt cggtcaaggt
     1141 tcgctgaccc ctcccgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc
     1261 caggacccgc tgctcgcgtc gaccaacctc acggccacgt ccccgtcgaa gggggtcacg
     1321 tcgctcacct ggaccaaccg gacggtcatg atcagcacgt tgcaggtcga gcgatgcacc
     1381 ggctccaggt gcaccaactt cgtcccggtc tcgtcagtcc cggggacctt cacgagcttc
     1441 tccgagtcgg gtctgaggtc ctcgacggtc ttccgctacc ggatccgggc caccgactac
     1501 ctcggcaaca cagccgtctc ctcgatcgtc tcggtcaaga cccgctgaca ccacgctaat
     1561 gccgggtcga ggtattccgc cgtcgtctgc tgatggccga atacctcgac ccggcaactg
     1621 gtaggacccg gcaggtggag acagcgcagc aggtatgccg cgtggcatac ctgaatctgc
     1681 gagatgctca gcgagtgatg tcgctggagg acgagcaggc gctacggcgt gaggccatgg
     1741 cgtggctcac ggtgcggacg aacgacggtc gggacgcgat cagtcgggag gacctcaccc
     1801 agttcaggtt ccgcggcgag agactcaccc tcatcgatca gggacgcggc atacgcaagc
     1861 cggcggcctg ccgcgcggcc ctctcgatca tgacggtgca cacgcccccg ggccgcgagc
     1921 gcccctacaa cgacatgcgt ggtccggacg gcctacctcg gtacaagatg cgggccgact
     1981 cgcgcagcgg cgcggacaac cgagcggtgc tgcaggcgtt ggagctgcac ctgcccgtga
     2041 tttggctggt tggagtggcg ccgggatggt tccaggtcgt gttcccggtc ttcgtcatcg
     2101 gcgccgagcc ggagctggat cagttcgttc tggcctatga cgcggcccag tcgttgccgc
     2161 tgctcggatc gcccatggag gaagtgcttc gccactacgt cactcgcgag acccgtcagc
     2221 gcgtccacca gccggtgttc cgttcgatgg tcatgctggc ctaccaaacg cagtgcgctg
     2281 tctgctccct ccggcacagc caactcctcg acgccgcgca catcatcccc gactccgacg
     2341 agggcgggat cgccgccgtg cgaaacggat tggcgctgtg caagattcac cacgcggcat
     2401 acgacgcagg catcctgggg atctcccccg actaccacgt ggcgatccgt gacgacgtgc
     2461 ttgccgaagt cgacggaccc ctgtttgagc acggcatcaa gggcctgcat ggccaaccgc
     2521 tgatggtcac accgagacgg cgggccgatc ggccggaccc ggagttgctc cagcagcgct
     2581 acgccgactt cctcagcgcc taagccgggt cgaggtatgt cgccatcatc gggggatggc
     2641 tgattacctc gacccagcgt tggggagcgt tgggaggtgt caaacacggc atagggtatg
     2701 ccgcgcggct gggctcaggg gcgggatccg ccggtgggcg cggtccgggt gtcgacggga
     2761 acggtgaggc gcacggcata cccactggcg gcgtccccag tcgaggcgcc gagctgatgc
     2821 acgccgccgt cgttgccgaa gacgttgtcg ctggtcagcg agagcttggc aaggttgccg
     2881 accgaggact cgtagcccgt cgtcgcgtag accgcgtcgc acgccgtctt gggcagggcc
     2941 acctgcgagg tggcgatcgc cttggtcgag tcgctgatgc tcgcctggtc ggggtagacc
     3001 tcgaagtgga tgtgcggcca gcgcccggag tagcagccgg ggaagatgct cgtgaacgag
     3061 acgacgccgt tcgcgtcggt gatctgcact ccgcgcaggt agttctcgtt ggtgatgccg
     3121 gaggagtaca tcgagtactg cccggcccgg tcgcagtgcc acgcgtagac ggcggcgccc
     3181 ttcatcgcgc caccgccgcc ggcgatgtcc aggatcgtga gctcgagggt catcgggacg
     3241 ccctcggcct tcgtcgtcga cgtgccgaag ctcgcggtga tgtccttgcg gacgacgccg
     3301 ctctgggcca gcacgtccgg cccgttggag ccgtcgcccg ggtaggggcc ggccgtctcg
     3361 tccgggatct cggtcagcgc gctcgtcccg ctggagctgg aactcgagct tgagctcgaa
     3421 ctggagctgg acttcgcgct cgtggtcgcc gcggcggatg tcgtggagcc ggtggacgtg
     3481 cccgagctgc aggccgccaa cgcggtcccg gccgcgccga gcccgaagag cgtcagcgcg
     3541 ttgcgccggt tgatcagcgt ggtgaggtcg aaggccaacc cctggtcgaa gggagcctcg
     3601 tcgggccggg gcagcggccg gccttcgaag gatgcggtcg tgtgttcgtc ggtcgtcatg
     3661 gcgggagact aggggatcaa tctaagcgct tgcagtgagt ttcctgtgag gctttgtggg
     3721 caccggtgaa ccctggcgcg tcacctgccc cgcgtagatt gaggctcgtg ccctacccgg
     3781 acatcgcgtt ctgccgcctc tgcggctccc cgtcggaccg tcgcgtgccg cagggggagg
     3841 accgggagcg gtcggtatgc cgtgcctgcg gctacgtcga ctacgtgaac cccacgaacg
     3901 tggtcggcac cgtgcccacc tggggccccg atggtgatca cgtgctgttg tgcctgcggg
     3961 ccatcgagcc gcggaagggc ttctggacct tgcctgctgg gttcctcgaa tacggcgag
//
LOCUS       DAORPW010000216         4015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7033_length_4015_cov_599.210354, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000216 DAORPW010000000
VERSION     DAORPW010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7033_length_4015_cov_599.210354"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLB86_12080"
     CDS             <1..768
                     /locus_tag="PLB86_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRC65925.1"
                     /translation="RRSLLTQKAYAEGGRALVLYTATMIDRFEVAKALGGNDTVAVER
                     HRLLLPIVKTWCAESGWRVLGQEALQVFGGSGYLRDYPLEQYVRDTKIDAIYEGTTGI
                     QSLDLFERRLVRDRGAIAGLLLDEIAQTVDDLATGALATESVLLGDALAAFRGILEHT
                     FAQADAAPRVAALGLTRLLMSMGDVIVGWLLLRTAAAALARQSDPSLLKTADADLAGS
                     VAAGRWFARQVLPHLTAELVAARLLDEEPLLLPDASL"
     gene            complement(858..2450)
                     /locus_tag="PLB86_12085"
     CDS             complement(858..2450)
                     /locus_tag="PLB86_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65926.1"
                     /translation="MALELGVNLPVGADGSRSSTASAQTVLAASVAGVDDTLAADIMR
                     ARDWRHRYPEYFTRILVAEAQSGSAALQIARQGLAAARSHYVVVAADGTQEPLPSAVT
                     RRGLDLRTVAVSGHDERVRELVVPHRGENLRGLALLRQLDDWVRRGIVEPTFADAVGA
                     VVRNPDWLDLRGHTFALVGAGAQMGPFAQLVQWGARVAAIDLPRPDVWKRLLSVVRES
                     AGTMYVPVHADHEDPVEADIPTRAGADLMTDVGPLVDWLAGLSGPMTVGNYGYADGVL
                     FVRLSMAFDALLEGLRSRRDDLSVTYLATPSDVFLVPMNAVDMAQERHSRQSPLIVAG
                     KVAHAMSAGRWFGPNYGRGGVIETPTERFGVVSAFIVAQGPNYAMAKRLQRWQMIATR
                     ADGILTSVHVAPPTRTSSVHSNPIMAERQRLTAYVGIETFDAPTTEAIAGAILVHDLH
                     NPASPANPANPLGHPHEAFMFAANPGGRWRVPFDVGSTVPLLHEITGARLRAGSLIRD
                     LSAHVPGLDRLPGLGRLGTSTD"
     gene            2549..3289
                     /locus_tag="PLB86_12090"
     CDS             2549..3289
                     /locus_tag="PLB86_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRC65927.1"
                     /translation="MNYLESVDNSLRLLMLVAAKARVGVSEVAAELGVAPSTAYRLLS
                     TLRYRGFVVQGHDRTYRAGPALEEMVSNRITRANLADLAAPALHTLRDVTEESTHLMV
                     LVGRQVRFIASVESFQSLRIGSRVGAMLPAHRTAGGRAILAALPTETLDQLYPTSGTT
                     SGELTASASATLRRDLARIRKQGYAVNNGETERGVTAVGSLVADVDGPLAALSVSIPT
                     IRYSSANLSGLVVALRAASDQIVATLRG"
     gene            complement(3341..>4015)
                     /locus_tag="PLB86_12095"
     CDS             complement(3341..>4015)
                     /locus_tag="PLB86_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65928.1"
                     /translation="GKPQNVVQMIFGGKMLQRTLLLWLGYACLMAAFYFANTWTPRLL
                     ITYLTNPANGGMDAKAAQAIGNNAGVLVAVGGVVGALLFALLARRVHPRVLTAYTLLW
                     GLAAYVIYANVFQTTSAAFMAAVGVGIAANGGVAAFYAISPSIYPTAARGTGVGWMVG
                     FGRIVSILAPIVSGYLIDGGMKSQSLYQIYGVILAIGAGLVFLLHRSMKSDSTSMTED
                     VPVSVH"
BASE COUNT          612 a         1345 c         1439 g          619 t
ORIGIN      
        1 cgtcggtcgc tgctcaccca gaaggcctat gccgagggtg gccgtgccct cgtcctctat
       61 accgcgacga tgatcgaccg gttcgaggtc gccaaggcgc tgggcggcaa cgacaccgtg
      121 gcggtcgaac gccaccgcct gctgctgccc atcgtcaaga cgtggtgcgc cgagagcggt
      181 tggcgggtcc tcggccagga ggctctccag gtcttcggtg ggtcgggcta tctgcgcgac
      241 tacccgctgg agcaatacgt ccgcgacacc aagatcgacg cgatctacga aggcacgacc
      301 ggcatccagt cccttgacct gttcgagcgg cgcctggtcc gcgatagggg cgccatcgcc
      361 gggttgctgc tcgacgagat cgcccagacc gtcgacgacc tggccaccgg tgctcttgcg
      421 accgaatcgg tcctgctggg agacgccctg gccgccttcc gaggaatcct cgaacacacc
      481 ttcgcgcagg ccgatgcggc gccccgggtc gcggccctgg gcctcacccg gttgctcatg
      541 tcgatgggcg acgtcatcgt cggctggctg ttgttgcgca ccgccgccgc ggcactggct
      601 cgccagagcg atccgagcct gctcaagacc gccgatgctg acctggccgg gtcggtggca
      661 gcagggcgct ggttcgcgcg gcaggtgctc ccgcacctca cggccgagtt ggtcgctgcg
      721 cgtctgctgg acgaggagcc gctgctcctc cccgacgcgt ccctgtgagc acaccgacgc
      781 cagggacgcc gccccacccc acgggaggct tcccgcctgc aggcgcaaga ggtatgccgc
      841 ccgcgcctgc cggcgggtca gtccgtcgac gtgcccaagc gccccaggcc aggcaggcgg
      901 tccagcccgg gaacgtgggc cgacaggtcg cggatcagcg atccggcgcg cagtcgggca
      961 ccggtgatct catgcagcag cggaacggtg ctgccgacgt cgaagggcac tcgccaccgt
     1021 cccccagggt tcgccgcgaa catgaacgcc tcgtgcgggt gtcccagcgg gttggccggg
     1081 ttggccggcg atgccgggtt gtgcaagtca tggaccagga tggcgccggc gatggcttcg
     1141 gtggtcgggg cgtcgaacgt ctcgatcccc acgtaggcgg tgagccgttg ccgctcggcc
     1201 atgatcgggt tggagtgcac ggagctggtc ctggtggggg gtgccacatg caccgaggtg
     1261 agtatgccgt ccgcccgggt cgcgatcatc tgccaccgct gcagacgctt ggccatggcg
     1321 taattgggcc cttgggcgac gatgaaggca ctgacgaccc cgaatcgttc ggtcggggtc
     1381 tcgatgaccc cgccccgacc gtagttgggc ccgaaccagc gccccgcgct catcgcatgg
     1441 gccaccttgc cggcgacgat gagcgggctt tgccgcgagt gtcgctcctg tgccatgtcg
     1501 acggcgttca tcgggacgag gaagacgtcc gagggggtgg ccaggtaggt gacggacagg
     1561 tcgtcccggc gcgaccgcaa gccttcgagc agcgcgtcga aggccatcga caggcgcacg
     1621 aagaggacgc catcggcata cccgtagttg ccgaccgtca tcgggccgct cagcccggcg
     1681 agccagtcga ccaggggtcc gacatcggtc atgaggtcgg cgcccgcgcg ggtggggatg
     1741 tcggcttcga ccggatcctc gtggtcggcg tggaccggga catacatcgt tccggccgac
     1801 tcgcgcacga cgctcagcag gcgcttccag acgtcgggcc gcggcagatc gatggcggcc
     1861 acccgggcgc cccactggac gagttgggcg aacgggccca tctgggcgcc ggcgccgacg
     1921 agggcgaagg tgtgaccgcg caggtccagc cagtcggggt tgcgcacgac cgccccgacc
     1981 gcgtcggcga aggtcggctc aacgatgccg cggcgcaccc agtcgtcaag ttggcgcaac
     2041 agcgccagac cccgaaggtt ctcgccgcgg tggggaacga ccagctcgcg cacccgctcg
     2101 tcgtgaccgc tgacggcgac ggtccgcagg tccaggcccc ggcgcgtcac tgcggagggc
     2161 agcggctcct gggtgccgtc cgccgcgacg acgacgtagt ggctccgtgc ggcggccaac
     2221 ccctggcggg cgatctgcag ggcggccgac ccggactgcg cctcggcgac gaggatccgg
     2281 gtgaaatatt cgggatagcg gtggcgccag tcgcgggcac gcataatgtc agcggcgagt
     2341 gtgtcatcga cgccggcgac cgaggccgca agcacggtct gggcgctggc ggtgctgctg
     2401 cgcgagccgt cggcgccgac ggggagattg acgccgagtt caagtgccat gcggatctgc
     2461 tttctccgga gggtttccac ctcgcaaccc ctggccgtaa cgtgtcctgg gccacatctt
     2521 cgactggagg aacgggggtc gtgcgcggat gaactacttg gagtctgtcg acaactcctt
     2581 gcgcctactc atgctcgtcg ccgccaaggc tcgggtgggt gtgagcgaag ttgcggccga
     2641 gctcggggtg gcgccgtcga cggcataccg actcctgtcc accctgcggt atcgggggtt
     2701 cgtcgttcag gggcacgacc gcacctaccg ggccgggccg gcgctcgagg agatggtgag
     2761 caaccggatc acccgggcca acctcgccga tcttgctgcg ccggcgttgc acaccttgcg
     2821 cgacgtcact gaggagtcca cccacctcat ggtgctggtg ggccgtcagg tgcgcttcat
     2881 cgcgagcgtc gagtcgttcc agtcgctgcg gatcggtagc cgcgtggggg cgatgcttcc
     2941 ggcccatcgc acggccgggg gccgcgccat cctggccgcc ttgcccaccg agaccctgga
     3001 tcagctctac cccacctccg gaaccacctc gggcgagctg accgcctccg cgagtgcgac
     3061 gttgcgtcgc gacctcgccc ggatccgcaa gcaggggtat gccgtcaaca acggcgagac
     3121 cgaacgcggg gtgaccgcgg tgggaagcct ggtggccgat gtcgacggcc cgttggccgc
     3181 cctctcggtc tcgatcccga cgattcgtta ctcgtcggcc aacctgtcgg gcctggtcgt
     3241 ggccctgcgc gctgcgagcg accagatcgt ggccacgttg cgcgggtgac cctggccctc
     3301 gggacgccca ccgtgctggc gggcgtcccg aggccacagg tcagtggacc gagacgggga
     3361 catcctcggt cattgacgtc gagtctgact tcatcgaacg gtgcaggagg aagaccaggc
     3421 ctgcaccgat cgcgaggatg accccgtaga tctggtagag gctctgcgac ttcattccgc
     3481 cgtcgatgag gtaaccggac acgatcgggg cgaggatgga gacgatccgg ccgaagccga
     3541 ccatccagcc gacgcccgtt ccgcgggctg cggtcgggta gatgctcggg ctgatggcat
     3601 agaacgcagc cacgccaccg ttggcggcga tgccgacgcc gacagctgcc atgaaggcgg
     3661 cgctggtggt ctggaagacg ttggcgtaga tgacgtaggc cgccaacccc cacaacaggg
     3721 tgtatgccgt gagcacgcgg gggtggaccc ggcgggcgag gagggcgaac aagagcgcac
     3781 cgacgacacc accgaccgcg accaggacgc cggcgttgtt gccgatggcc tgggcggcct
     3841 tggcgtccat gcccccgttg gccgggttgg tgaggtacgt gatgagcagg cgcggggtcc
     3901 aggtgttggc gaagtagaac gccgccatga ggcacgcgta gccgagccag agcagcagag
     3961 tgcgctggag catcttgccg ccgaagatca tctggacgac gttctgcggc ttacc
//
LOCUS       DAORPW010000217         4002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7068_length_4002_cov_904.573854, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000217 DAORPW010000000
VERSION     DAORPW010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7068_length_4002_cov_904.573854"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..437)
                     /locus_tag="PLB86_12100"
     CDS             complement(135..437)
                     /locus_tag="PLB86_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65929.1"
                     /translation="MLVHVEPDDLAAAGHRMSGDGPRLRALAQLISGCGVAGRRACPG
                     APALGQALAALGERGSAAISTLAVATDTLAAGLGDAGRRYGDVEDRLAVMGRRVIG"
     gene            complement(526..2166)
                     /locus_tag="PLB86_12105"
     CDS             complement(526..2166)
                     /locus_tag="PLB86_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRC65930.1"
                     /translation="MHSPASDWWRDAVIYQVYPRSFSDSDGDGTGDLPGVTARLPHLA
                     DLGVDGLWLSPFYRSPLADAGYDVADYRDVDPRFGTLADVDTLIARAHELGLAVLVDL
                     VPNHSSDQHPWFQAALAAGPGSPERARYLFRDGRGANGELPPNNWPSVFGGQGWTRVT
                     EADGRPGQWYLHLFDVHQPDFDWTAPEVRAEFESVLRFWLDRGVAGFRVDVAHGLIKA
                     PGLPDLSAPLDELELLDTNATPFWDQDGVHEVYAAWRRLLDSYGAPERVLCAEAWVSP
                     PERMARYVRSDEMHQSFAFDFLGCRWDAKALRKVIDAELQTMGDVGAPITWVLSNHDV
                     VRHASRLGTADPGPVVGGIRATDPQPDRALGLRRARAATLLMLALPGAAYLYQGEELG
                     LPEHTTLPDAVRDDPVWLRSEHTHAGRDGCRVPLPWVADAPGFGFSPTGASWLPQPAA
                     FAEYAADVQTGVPGSTLEMYREALVLRREHRLGRGRLVWVANAPAGTLEFDNGDVRVI
                     VNCSAPAVALPADRVVAAQSGPLTADGMLPINTAVWLV"
     gene            complement(2174..2611)
                     /locus_tag="PLB86_12110"
     CDS             complement(2174..2611)
                     /locus_tag="PLB86_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HRC65931.1"
                     /translation="MSDNVQGVSEGQAPLGRETSFYDLVGGHDTFVRLVHGFYEGVAG
                     DPVLRALYPEEDLGPAEIRLRMFLEQYWGGPNTYSEERGHPRLRMRHTPYAVTPEQRD
                     RWLHHMLASVALLKLDREQEATLVDYLVRAAYMLVNAPDPEKD"
     gene            complement(2616..3539)
                     /locus_tag="PLB86_12115"
     CDS             complement(2616..3539)
                     /locus_tag="PLB86_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRC65932.1"
                     /translation="MTAYPLLVSPAAWQVTLDQLLGTPLQIFGIVLTAVIGRWLLHRL
                     IRTVVNRATAQHSDRLRILRGRAGEALADASGLADERYVQRTQTVGAVLRSVVTIVVS
                     TLAVLTIMATLGVPLAPLLASAGVGGVALGFGAQSLVRDFLSGIFMIVEDQYGVGDTI
                     DTGEVIGTVEDVSLRVTRLRDRNGVIWYVRNGEILRVGNHSQGWSIAAIAVPFALDAD
                     IAKVTQVLTAALEGLPDRDDRVIEDPSVVIESMSAGTITVRVSARCVPTVNLDVQRDL
                     RVQVKEALDAAGISMAAPVVVPSASPTTGTG"
     gene            complement(3536..>4002)
                     /locus_tag="PLB86_12120"
     CDS             complement(3536..>4002)
                     /locus_tag="PLB86_12120"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HRC65933.1"
                     /translation="ASGGASEDDIAAELDRDPSLAGREQALKARAAIPTAQAKTAAWA
                     LAIEQDTQTNAALEAIGAGFRRCDDPALLRPFVESYLDMLEPVFADRSYAIAERCVKY
                     FYPLDIADAALRDRTRAWLADHEDAPAGLRRLVIEQLAVVETAVAIQEREGS"
BASE COUNT          632 a         1414 c         1407 g          549 t
ORIGIN      
        1 cccccacccc tggccaccct cgcgggcgac gacggtctgg tcgaccgtag tggcaacgag
       61 cgccaattgg cttgcgatcc ggtcgatctc gctgacctcg ccagggggcc acgccaaagc
      121 gctgtcgcgg gtgctcatcc gatcacccgt cggcccatga ccgccagccg gtcctcgaca
      181 tcgccgtatc ggcgcccggc gtcacccagc cccgcggcca acgtgtccgt cgcgacggcg
      241 agggtgctga tcgcggcgga cccgcgctcg ccgagggcgg ccaaggcctg acccaacgcg
      301 ggcgcaccgg ggcaggcacg ccgcccggca acgccgcacc cggagatcag ctgagccagg
      361 gcgcgcaacc gagggccgtc gccagacatc cggtgtccgg cggcagcgag gtcatcgggc
      421 tcgacatgca ccaacatgct ggcaggctat gcgctcgcgc cgaccgtcgt cggaagttat
      481 ccacaggctg cgagcggccc gcctcgtccg ccgtgcggcc cgccgtcaga ccagccagac
      541 ggccgtgttg atcggcagca tcccgtcggc cgtcaacggc ccggactggg cggccacgac
      601 ccgatccgcg ggcagggcaa ccgctggggc gctgcagttg acgatcacgc ggacgtcgcc
      661 gttgtcgaac tccagcgtcc ccgcgggagc attcgccacc cacaccagcc gaccccggcc
      721 gaggcgatgc tcgcgccgca ggaccaatgc ctcccgatac atctcgagcg tcgagccggg
      781 cacgccggtc tggacgtctg cggcatactc ggcgaacgcg gccggctgcg gcagccaact
      841 ggcaccggtg ggggagaagc cgaagccagg agcgtcggcc acccacggca gcgggacccg
      901 gcagccgtcg cggccggcgt gggtgtgttc gctgcggagc cagaccgggt cgtcgcgaac
      961 cgcgtcgggg agggtggtgt gctccggcag gccgagctcc tcaccctgat agaggtatgc
     1021 cgcccccggc aacgcgagca tgagcagcgt tgccgcccgc gcgcgccgca ggcccaacgc
     1081 ccggtcaggc tgcgggtcgg tcgcccgaat cccgccgacg acgggccccg ggtcggccgt
     1141 gcccaagcgc gaggcgtgcc gaacgacgtc gtggttggac agcacccacg tgatcggagc
     1201 tccgacgtcg cccatggtct gcagctcggc gtcgatgact ttgcgcaatg cctttgcatc
     1261 ccaacgacat ccgaggaagt cgaaggcgaa gctctggtgc atctcgtcgg atcgcacata
     1321 gcgggccatc cgctccggcg ggctcaccca cgcctcggcg cagaggacgc gctccggtgc
     1381 gccgtaggag tcgagcaggc ggcgccacgc ggcatacacc tcgtgcacgc cgtcctggtc
     1441 ccagaagggg gtggcgttcg tgtcgagcaa ctcgagctca tccaggggag cgctcagatc
     1501 aggcagcccc ggagccttga tgagcccgtg ggcgacgtcg acccggaagc cggccacccc
     1561 gcgatcgagc cagaaccgca gcaccgactc gaactcggcg cgcacctccg gcgcggtcca
     1621 gtcgaagtcg ggctgatgga cgtcgaagag gtgcaggtac cactgacccg gtcgcccgtc
     1681 ggcttcggtg acgcgggtcc acccctgacc accgaacacc gacggccaat tgttcggcgg
     1741 cagttcgccg ttcgcgccgc gcccgtcgcg gaagaggtaa cgcgcacgct ccggcgaccc
     1801 cggtccggcg gccaacgcgg cctggaacca cgggtgttgg tcggacgagt ggttgggcac
     1861 gaggtcgacc agcaccgcga gcccgagctc gtgggcccga gcgatgagcg tgtcgacatc
     1921 cgcgagcgtc ccgaagcgtg ggtcgacgtc tcgatagtca gccacgtcgt aaccggcgtc
     1981 ggcgagcggc gagcggtaga acggcgagag ccagagaccg tcgacgccga ggtcggccag
     2041 atggggcagc cgcgccgtca cccccggaag gtcaccggtg ccgtcgccgt cggagtcgct
     2101 gaacgagcgc gggtagacct ggtagatcac cgcgtcgcgc caccagtcgc tcgcggggga
     2161 gtgcatcgtc acgtcagtcc ttctcggggt caggagcgtt caccagcatg tatgccgccc
     2221 gcaccaggta gtcgaccagc gtcgcctctt gctcgcggtc cagtttcaga agcgccaccg
     2281 aggcgagcat gtggtgaagc cagcgatcgc gttgctcggg ggtcacggca tacggagtgt
     2341 ggcgcatccg caggcgtggg tgcccgcgct cctcggagta ggtgttgggg ccgccccaat
     2401 actgctcgag gaacatccgc agccggatct cggccgggcc gagatcctct tcgggataca
     2461 gcgcccgcag caccggatca ccggcgacgc cctcgtagaa cccgtggacg agtcgcacga
     2521 acgtgtcgtg tccgccgacg aggtcgtaga aactggtctc gcgtcccagc ggcgcttggc
     2581 cctcggacac gccctgcacg ttgtcgctca ctcgttcacc ctgtccccgt cgtcggcgac
     2641 gccgatggta cgaccactgg ggcagccatg gagattccgg ccgcgtcgag ggcctccttg
     2701 acctgcaccc ggaggtcgcg ctgcacgtcg aggttgaccg tcggcacgca tcgggccgag
     2761 acccgcaccg tgatcgtgcc ggccgacatc gactcgatga cgaccgaggg gtcctcgatc
     2821 acccggtcat cccgatcggg caggccttcc agggccgcgg tgagcacctg agtcaccttg
     2881 gcgatgtcag cgtcgagggc gaagggcacc gcgatcgcgg caatcgacca cccctgcgag
     2941 tggttgccca cccggaggat ctcgccgttg cggacgtacc agatgacgcc gttgcgatcc
     3001 cgcaggcggg tgactcgcag gctgacgtct tcgacggtgc cgatgacctc cccggtgtcg
     3061 atggtgtccc cgacgccgta ttggtcctcg acgatcatga agatccccga gaggaagtcg
     3121 cgcaccaggc tctgcgcccc gaaaccgagc gccaccccgc cgacgccggc cgaggccaac
     3181 aacggggcaa ggggcacgcc gagcgtcgcc atgatggtga ggaccgccaa ggtggagacg
     3241 acgatcgtga ccacgctgcg cagcacggcg ccgacggtct gggtccgctg gacgtagcgc
     3301 tcgtccgcca gcccgctggc gtcggcgagg gcctcacccg cccgtccgcg caggatccgc
     3361 agtcggtcgc tgtgctgggc ggtggcccgg ttgacgaccg tccggatcag tcggtgcagc
     3421 aaccagcgac cgatgaccgc ggtgaggacg atgccgaaga tctgcagcgg cgtgccgagc
     3481 agctggtcga gggtcacctg ccacgcggcg gggctgacga gcagggggta tgccgtcacg
     3541 aaccctcgcg ttcttggatc gccaccgcgg tctccaccac ggccagctgc tcgatcacca
     3601 gtcggcgcag gccagcgggg gcatcctcgt ggtcggccag ccacgcgcgg gtccggtcgc
     3661 gcagggccgc gtcggcgatg tccagcgggt agaagtactt cacgcaccgc tcggcgatgg
     3721 cataggagcg gtccgcgaag accggctcga gcatgtcgag gtagctctcg acgaacggac
     3781 gcagcagggc cggatcgtca cagcggcgga atccggcgcc gatggcctcg agggcggcgt
     3841 tggtctgggt gtcctgctcg atggccaagg cccacgcggc agtcttcgcc tgcgccgtcg
     3901 ggatagcggc gcgagccttg agcgcctgct cccgaccggc cagcgaaggg tcgcggtcca
     3961 actcggcggc gatgtcgtcc tcagaggcgc cgccggaagc gg
//
LOCUS       DAORPW010000218         3996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7084_length_3996_cov_725.227353, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000218 DAORPW010000000
VERSION     DAORPW010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7084_length_3996_cov_725.227353"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLB86_12125"
     CDS             <1..192
                     /locus_tag="PLB86_12125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851607.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRC65934.1"
                     /translation="SQNTYAATQFTGIEGSTVPLADTIEAFTKISDGDYDHVAEQAFF
                     MCGGLDDVERQWAEIQKNL"
     gene            345..986
                     /locus_tag="PLB86_12130"
     CDS             345..986
                     /locus_tag="PLB86_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65935.1"
                     /translation="MPESAQDYYDRILAATDADGRLPLAVEAMPGWDIFPYELDSLRL
                     KPLQPLLEREPDRQGEDPASCRCAQGQPSEGVIWSDEHWRVSAERSGLPITAVVVSRE
                     HHDLADLPAELASEFGRLLVVIAAAIEALPSVGRAHLGRYGDGGAHLHVFLFGRPARA
                     GQFRGSPLLDWEENLPKIPVTVAAENARFVADRIVATYGGRAHTPAAGGPEDR"
     gene            1033..1296
                     /locus_tag="PLB86_12135"
     CDS             1033..1296
                     /locus_tag="PLB86_12135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRC65936.1"
                     /translation="MSELQVELVAADRRVWVGAAKMVRCRTSSGELGVMPGHTPMLGI
                     LVNGELIIHQADGAKQAATIDSGFLSVEHDRVTIVADHVDVQG"
     gene            1309..1746
                     /locus_tag="PLB86_12140"
     CDS             1309..1746
                     /locus_tag="PLB86_12140"
                     /inference="COORDINATES: protein motif:HMM:NF022199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2550 domain-containing protein"
                     /protein_id="HRC65937.1"
                     /translation="MPDLIFSVEVVVGIVVAVVVGYFLAVILRRRAISRGRLLAMCAY
                     AREGTHGWRNGFIRFGEGDVEWYPMGGISVRPKHAWSRRSLDLGTPVPVGRDEGLDFI
                     DDGVSVLCHDGTGAFRLGMARSAYTALRSFVESAPPESASSVN"
     gene            complement(1811..2443)
                     /locus_tag="PLB86_12145"
     CDS             complement(1811..2443)
                     /locus_tag="PLB86_12145"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRC65938.1"
                     /translation="MVNLTRIYTRTGDDGTTALGDFSRTPKTDPRLAAYADTNEANAC
                     LGVAVAAGGLREDLRTTLVRIQNELFDVGADLCLPLRETYDYPPLRVQQPWIDDLEAD
                     CDRYLAELQPLRSFILPGGSAGAAYLHVATTVVRRAERATWAAIEHYGTDLGVERGEG
                     GVNPLTATYLNRLSDLLFVLARVANLPDGDILWQPGGGRVEAPKPRGSRD"
     gene            2698..3030
                     /locus_tag="PLB86_12150"
     CDS             2698..3030
                     /locus_tag="PLB86_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRC65939.1"
                     /translation="MEVSEVAAGVRVRVGGRLDAHSVAHVREVLHGVIDAGTGPVLIE
                     LPDAEIGDATALGLLVGAHHRARRLGRELYVGEVSERTARLLRMSHLDRVLAQAPEAM
                     RTVAALTA"
     gene            3071..>3996
                     /locus_tag="PLB86_12155"
     CDS             3071..>3996
                     /locus_tag="PLB86_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRC65940.1"
                     /translation="MAETPSAAARPQGEPDAPARPTRERPWVIRTYAGHSSAAASNAL
                     YRRNLEKGQTGLSVAFDLPTQTGYDPDHVLSRGEVGKVGVPISHIGDMSALFEGIPLK
                     AMNTSMTINATAMWMLAMYQVTAEAQAEAAGEDPSAWVAALTGTTQNDIIKEYLSRGT
                     YAFPPAPSLRLITDMIAYTVSQIPKWNPTNICSYHLQEAGATPVQEIAYSMCTAIAVL
                     DAVRDSGQVPPERFGEVVARISFFVNAGVRFVEEMCKMRAFVQLWDEITQERYAVSDP
                     VARRFRYGVQVNSLGLTEAQPENNVQRIVLEML"
BASE COUNT          601 a         1386 c         1371 g          638 t
ORIGIN      
        1 tcgcagaaca cgtatgccgc cacgcagttc accggcatcg agggctcgac ggttccgctg
       61 gccgacacga tcgaggcctt caccaagatc agtgacggcg actacgacca cgtcgcggag
      121 caggcgttct tcatgtgcgg tggcctcgac gacgtcgagc gccagtgggc cgagatccag
      181 aagaacctct gaccccacca gccgacgccc caacagcgtc gctcgagaag gtccgtcacg
      241 gggccggtca ccgcacgcgg tggccggccc cgcggcatac cccagcggtg ttggcgtgcc
      301 cagtcgaccc ctgggtatgc cgtgcgcctg ggtagggtcg ggccatgccg gaaagcgcgc
      361 aggactacta cgaccggatc ctggcggcca ccgatgccga cgggcggctg cctctggcgg
      421 tggaggccat gccggggtgg gacattttcc cctacgagtt ggattcgctg cggctcaagc
      481 cgctgcagcc tctgctggag cgcgagcccg accgtcaggg cgaggacccg gcctcctgcc
      541 gatgcgccca gggacagccc tccgaggggg tcatctggag cgacgagcac tggagggtca
      601 gtgcggagcg ctcgggactg ccgatcacgg ccgtggtggt gtcgcgcgag catcacgacc
      661 tcgccgatct gccggccgag ctggcgagtg agttcgggcg gctcctcgtc gtcatcgctg
      721 ccgccatcga ggcgttgccc tcggtgggcc gcgcccacct cggccgatat ggcgacgggg
      781 gcgcccacct gcatgtgttc ctcttcgggc ggcctgctcg ggccggccag ttccgtgggt
      841 caccgctgct ggactgggag gagaacctcc ccaagatccc ggtcaccgtg gcggcagaga
      901 acgcgaggtt cgtcgccgac cggatcgtgg cgacctacgg tgggcgcgcg cacaccccgg
      961 cagctggagg tccggaggac cgatagactc gggcagcagt agagggctga agccgatcgt
     1021 ccggagggca tcgtgagtga actgcaggtc gagctggtcg ccgcagaccg gcgcgtctgg
     1081 gtcggggcgg ccaagatggt gcgctgccgc acttcgtccg gcgagctcgg cgtcatgccc
     1141 ggccacacgc cgatgctggg catcctcgtc aacggcgagt tgatcatcca ccaggccgac
     1201 ggcgccaagc aggccgccac catcgacagc gggttcctct ccgtcgagca tgaccgagtg
     1261 acgatcgtcg ccgaccacgt cgacgttcag ggctgaccgg agccgctcgt gcccgacctg
     1321 atcttttccg tcgaggtcgt ggtcgggatt gttgtggccg tcgttgtcgg ctacttcctc
     1381 gccgtcatcc tgcgccgccg ggccatctcg cgcgggaggc tgttggccat gtgcgcctac
     1441 gcccgcgagg ggacccacgg gtggcgcaac gggtttatcc gcttcggcga gggtgacgtc
     1501 gagtggtatc caatgggcgg catctcggtg cgcccgaaac acgcctggtc acggcgcagc
     1561 ctcgacctcg gcaccccggt ccccgtaggg cgcgacgaag gtctggattt catcgacgac
     1621 ggcgtgtcgg tcctgtgcca cgacggtacg ggcgccttcc gcctgggcat ggctcgatcg
     1681 gcctatacgg ccttgcgttc gttcgtcgag tcggcgccgc cggagtcggc gtcgtcggtt
     1741 aactgaccgc tcaccgccga gtcagcgagt cgcctcgtcg gcaagtcgcc tcatcagcga
     1801 gtcggacccc tcagtcgcga gagccgcggg gcttgggcgc ttccactcgg cctccgccgg
     1861 gctgccagag gatgtcgccg tcgggcaggt tggccactcg agccaacacg aacagcaggt
     1921 cagagagccg gttgaggtag gtcgccgtca gggggttgac gccgccctcg ccgcgctcga
     1981 cccccagatc ggttccgtag tgctcgatgg ccgcccacgt cgctcgctcg gcccgtcgca
     2041 ccacggtcgt ggcgacgtgt aggtatgccg cacccgccga ccctccgggg aggatgaacg
     2101 agcgcagcgg ctgcaactcg gccagatagc ggtcacagtc ggcttccagg tcgtcgatcc
     2161 acggctgctg gacccgcagc ggcgggtagt cgtaggtctc gcgcaacggc aaacacaggt
     2221 cggcgccgac gtcgaacaac tcgttctgaa tgcgcaccag cgtggtccgc aggtcttccc
     2281 gcaggcctcc ggcagcgacc gcgacgccca ggcaggcgtt ggcctcgttc gtgtcggcgt
     2341 atgccgccag ccgcgggtcg gtcttcgggg tccggctgaa gtcgcccagc gcggttgtcc
     2401 cgtcgtcgcc ggtgcgggtg tagatgcggg tgaggttgac catgacgccc agcctgtcag
     2461 atccgggccg acgcgcgggt cggacgctcg cagatcccag acgtcacggc atacacttgt
     2521 gtctctttcg taacatccgt cacagcgtgt ctctggcgtc gctcacccgg ctcctcctct
     2581 agcgtgacga tccgaagggc gcgccgacgc caccgacaca tgctttccgt cgccacaggg
     2641 ggtgaccgca cgatgccgac tcgacacagc catgccgggt ctcctcgcgg tgtccccgtg
     2701 gaggtgtcgg aggtggctgc gggcgtgcga gtgcgggtcg gcggccgact ggacgcacac
     2761 tcggtggccc acgtgcgcga ggtcctgcac ggagtgatcg atgccggcac cggcccggtc
     2821 ctcatcgagc tgccggacgc cgagatcggc gacgccacag ccctcggcct gctcgtcggc
     2881 gcgcatcacc gggcccggag gttgggccgc gagctctacg tcggcgaggt gtccgagcgc
     2941 accgcccggc tcctgcggat gtcgcacctg gatcgggtgc tcgcccaggc gccggaggcc
     3001 atgcgcactg tggcagcact cacggcctga ccctgggcag gtggggctac ctgccggtag
     3061 cgtcagatcc atggccgaga caccttccgc tgccgcacgc ccccagggcg aacccgacgc
     3121 gcccgctcga cccaccagag agcgcccctg ggtgatccgc acgtatgccg gtcactccag
     3181 cgccgccgcc agcaacgccc tctaccggcg caacctggag aagggccaga ccggcctgtc
     3241 ggtcgccttc gacctgccca cccagaccgg ttacgacccc gaccacgtcc tgtcccgggg
     3301 tgaggtcggc aaggtcggcg tgccgatcag ccacatcggc gacatgtcgg cgctcttcga
     3361 gggcatcccg ctcaaggcca tgaacacctc gatgacgatc aacgcgacgg ccatgtggat
     3421 gctcgcgatg taccaagtga ccgccgaggc ccaggccgag gccgccggcg aggacccgag
     3481 cgcgtgggtg gcggccctca ccgggaccac tcagaacgac atcatcaagg aatacctctc
     3541 gcgggggacg tatgcgttcc cgcccgcgcc gtcgctgcgg ctgatcaccg acatgatcgc
     3601 ctacacggtc tcgcagatcc ccaagtggaa cccgacgaac atctgctcct accacctgca
     3661 agaggccggt gcgacgccgg tgcaggagat cgcctactcc atgtgcacgg cgatcgccgt
     3721 cctggacgcg gtgcgcgact ccggtcaggt gccgccggaa cgcttcggtg aggtcgtcgc
     3781 tcggatctcg ttcttcgtca acgccggcgt gcgcttcgtc gaagagatgt gcaagatgcg
     3841 cgcgttcgtc cagttgtggg acgagatcac ccaggagcgg tatgccgtgt ccgacccggt
     3901 cgcgcgccgg ttccgttacg gggtgcaggt caactccctc gggctcaccg aggctcagcc
     3961 ggagaacaac gtccagcgga tcgtcctgga gatgct
//
LOCUS       DAORPW010000219         3984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7112_length_3984_cov_725.707305, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000219 DAORPW010000000
VERSION     DAORPW010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7112_length_3984_cov_725.707305"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /gene="arsM"
                     /locus_tag="PLB86_12160"
     CDS             complement(<1..876)
                     /gene="arsM"
                     /locus_tag="PLB86_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRC65941.1"
                     /translation="MSQSSSPAGPHTSTAGLDTPAEQVRSVVQERYAAAARTTLDLLE
                     GTETAAGSCCAPSCCAEPAAADPITHTETAAAPCCGSSCCATPTGADPITRDLYEPGE
                     VPSAGALVASLGCGNPTLLADLQPGQSVLDLGSGGGLDVLLSARRVAPGGTAYGLDMT
                     PEMLELARRNQAEAGVENATFLLGTIEEVPLGDASVDVVISNCVINLSPDKDAVLREA
                     FRVLRPEGRFAVSDIVLLRPIPEHLTGIVGLWTGCVSGALLDSDYVAKLGAAGFVDAS
                     VEVTRTYSRIDLEDLA"
     gene            949..1293
                     /locus_tag="PLB86_12165"
     CDS             949..1293
                     /locus_tag="PLB86_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRC65942.1"
                     /translation="MTTTRLELAAPCCALGAPPVSPADAEAIAPMFKALGDPVRLRLV
                     SLIAAQPEACVCDLVGAFPLSGPTISHHLKVLRAAGLVDCERRGTWVYYWVRPEALAR
                     LSSLLGRPISPA"
     gene            1398..2897
                     /locus_tag="PLB86_12170"
     CDS             1398..2897
                     /locus_tag="PLB86_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRC65943.1"
                     /translation="MSPTPVPDPDLLPRLRADLTAAAYTVDGVEHTLGPVAAGALHRE
                     QRLPADLATRHRPEPAAVLVRLFALGFPVAWDDADRALPTLRADGAVQLGLARAEGAD
                     LRSTCDLRPYADESHQWWVASDLSEIATGRPLAETHVLGIGNASTTLAEWTIRRQARR
                     ALDLGVGSAVQSLHLATHAEGIVGTDVSERALAFARFNADLNDLAIELRPGSLLDPVA
                     GERFDLIVSNPPFVITPRSAGVPLYEYRDAGLVGDGVVEALVRRVGEHLEPGGVAQFL
                     GNWEIRRGADWRDRWHEWLDGTGLDAWVIQRDVQDPAQYAELWARDAGARSGAPNFDA
                     MYAAWLADFADRHIEGIGFGIITLQRPLGDREPWLDLVEVPGPVAPGVGHAVDAGLRA
                     RTAIAEGGTAYLLDTPWSAAPDVTEERHLLPGAEDPSVILARQGGGLRRTVQLDTVAA
                     GLIGVCDGSLTPRVALAAIASLLELDDAHVTEVAVPTLRALVADGFLVA"
     gene            complement(2912..3607)
                     /locus_tag="PLB86_12175"
     CDS             complement(2912..3607)
                     /locus_tag="PLB86_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65944.1"
                     /translation="MLDPTKTTLPKQWASEFAMALRSRDVTGAAIGDALREAEAYCAD
                     SGQAPLEAFGPAAAYADSLTDLPTAAAHTSWAARIAPSALGLAGLTLALPTFDAWRAG
                     RSVQLPLGGLVTLLVIAATVTVFLVWPRTFRRAVPFLILVSLGMWGSVSATALLQQPV
                     ATLPTVPSALVAAGALIGSGIWQMRTLDLDVIVDPLGPTPRPGRGFLLLTAWLLPIGT
                     LVAMGIAMFAPVR"
     gene            complement(3634..>3984)
                     /locus_tag="PLB86_12180"
     CDS             complement(3634..>3984)
                     /locus_tag="PLB86_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRC65945.1"
                     /translation="RGVLEMAVLRVLANEPSSYGYAISVALDRNGLGTVKGGTLYPLL
                     ARLEQAAHVETRWEAGDGGPGRKYYTITDSGRSHLESAAASWCAFAETTASFLRAEAP
                     GIPAPSPATTVPAG"
BASE COUNT          560 a         1433 c         1409 g          582 t
ORIGIN      
        1 cgcgaggtcc tcgaggtcga tccgggagta ggtccgggtg acctcgaccg acgcatcgac
       61 gaaccccgcc gcgccgagtt tcgccacata gtcggagtcc aacagggccc ccgacacgca
      121 cccggtccac aggcccacga tgcccgtgag gtgctccggg atcggccgca gcagcacgat
      181 gtccgacacc gcgaaccgcc cctccggtcg cagcacgcgg aacgcctcgc ggagcaccgc
      241 gtccttgtcg ggcgacaggt tgatgacgca gttggagatg acgacgtcga ccgacgcgtc
      301 gcccagcggc acctcctcaa tcgtcccgag caggaacgtc gcgttctcga cccccgcctc
      361 ggcctggttg cgacgggcca gttccagcat ttccggggtc atatcgagcc cgtatgccgt
      421 cccgccgggt gccactcgcc gcgcggacag caggacatcc agtccgccac cggagccgag
      481 gtcgaggacg ctctgcccgg gctgcaggtc ggccaatagg gtcgggttgc cgcagccgag
      541 ggaggcgacg agggcgccgg ccgacgggac ctcgccgggc tcgtagaggt cgcgcgtgat
      601 cgggtcggcc ccggtcggtg ttgcgcagca cgaggaccca cagcacggcg cggcagccgt
      661 ctcggtgtgc gtgatcgggt cggccgcagc gggttccgcg cagcacgagg gggcgcaaca
      721 ggatccggcg gcggtctcgg tgccctcgag caggtcgagg gtcgtgcggg cggccgccgc
      781 atagcgctcc tggacgaccg agcggacttg ctccgccggg gtgtcgaggc ccgccgtcga
      841 ggtgtgcggg ccggcagggc tggatgactg gctcatggtc tctccttgtt tcgatgactg
      901 tcgaaacacg atgctgcatc ttgtttcgat atctgtcaag ataggcccat gacaacgacc
      961 cggttggagt tggcggcccc ctgctgcgcg ctcggtgcgc cgcccgtgtc gcccgccgac
     1021 gccgaggcga tcgccccgat gttcaaagcg ctcggcgacc cggtgcggct gcgcctcgtg
     1081 tcgctcatcg ccgctcaacc cgaggcctgc gtctgcgatc tcgtgggcgc cttcccgttg
     1141 tccgggccga cgatctccca ccacctcaag gtccttcggg ccgccgggtt ggtcgactgc
     1201 gagcgtcggg gcacctgggt ctattactgg gtgaggccag aggcgttggc ccggctcagt
     1261 tccctgctcg ggcgtcccat ctcacctgcc tgaccccgcc tcgccagtcc gacccccagc
     1321 tgattcgagg tattccacca tcgccgggta acggccgaat acctcgaccc ggcagggggc
     1381 ggcataccct tgtcgccgtg agcccgacgc ctgtgcccga ccctgatctc ctgccgcgcc
     1441 tgcgcgccga cttgactgct gcggcataca cggtggacgg ggttgagcac acgcttggcc
     1501 cggtggctgc gggcgctctg caccgcgagc agcgccttcc ggccgacctg gccacccgcc
     1561 accgccccga gcccgccgcg gtgctcgtgc ggctcttcgc cctgggcttc ccggtcgcgt
     1621 gggacgacgc cgaccgcgcg ctgcccacgc tgcgggccga cggggccgtc caactcgggc
     1681 tggcccgcgc tgagggcgcc gacctgcgct cgacctgcga cctgcgcccc tacgccgacg
     1741 agtcgcacca gtggtgggtc gcctccgacc tctcggagat cgcgaccgga cgtccactgg
     1801 ccgagaccca cgtcttgggg atcgggaacg cctcgaccac cctcgctgag tggaccatcc
     1861 ggcggcaggc acgtcgggcg ctcgacctgg gggttggcag cgccgtccag tcgttgcacc
     1921 tggccaccca cgccgagggc atcgtcggca ccgacgtctc cgaacgcgcc ctggccttcg
     1981 cccggttcaa cgccgatctc aacgacctgg ccatcgagct gcggcccggc agtttgctgg
     2041 atcccgttgc cggagaacgc ttcgacctga tcgtgagcaa cccgcccttc gtcatcacgc
     2101 cccgatcggc cggggtgccg ctctatgaat accgcgacgc aggcctggtc ggcgacgggg
     2161 tcgtcgaggc gctcgtccgc cgggtggggg agcacctcga gcccggtggc gtcgcccagt
     2221 tcctcggcaa ctgggagatc cgtcgcggcg ccgattggcg cgatcgctgg cacgagtggc
     2281 tcgacggcac cggcctggac gcctgggtca tccagcgtga cgtgcaggac ccggcgcagt
     2341 atgccgagtt gtgggctcgc gacgccgggg cccgttccgg cgcaccgaat ttcgacgcga
     2401 tgtatgccgc gtggctggcc gacttcgccg accgccacat cgagggcatc ggcttcggca
     2461 tcatcaccct gcagcggccg ctcggcgacc gcgagccgtg gctcgacctc gtcgaggtcc
     2521 ccggaccagt ggctcccggg gttggccatg cggtcgacgc cggcctgcga gcccggaccg
     2581 cgatcgccga aggcgggacg gcatacctgc tggacacgcc gtggagcgcg gcgccggacg
     2641 tcaccgagga gcgacacctg ctgccgggtg ccgaggaccc gtcggtgatc ctcgcccgtc
     2701 agggcggcgg cctgcgccgc accgtccaac tcgacaccgt ggcggccggt ctcatcggcg
     2761 tctgcgacgg ctcactgacg ccgcgggtgg cgctggcggc gatcgccagt ttgttggagc
     2821 tcgacgacgc gcacgtcacc gaggtcgccg ttcccaccct ccgggccctg gtcgccgacg
     2881 ggttcctcgt cgcctgaggc gtcctgcgga tctagcgcac cggggcgaac atcgcgatgc
     2941 ccatggccac gagcgtgccg atcggcaaca gccaggccgt gagcagcagg aacccgcgac
     3001 cagggcgcgg cgtcggtccc aagggatcga cgatcacgtc cagatccaag gtcctcatct
     3061 gccagatgcc cgaaccgatg agcgccccgg cggcgacgag ggcgctcggc acagtgggga
     3121 gggtcgcgac cggctgctgg agcagcgccg tggccgacac cgagccccac atgcccaggc
     3181 tcacgaggat gaggaacggc accgcgcgac ggaaggtccg cggccagacg aggaagacgg
     3241 tcaccgtcgc cgcgatgacg agcagggtga ccagaccgcc aagcggcaac tgcacgctgc
     3301 gccccgctcg ccaggcatcg aacgtcggca gggcaagggt gaggccggcc agaccgagcg
     3361 ccgacggggc gatccgcgcg gcccacgacg tgtgcgcggc ggcggttggc aggtcggtga
     3421 gcgaatcggc atacgccgca gccgggccga acgcctccag aggtgcctgg ccgctgtctg
     3481 cgcagtaggc ctccgcctca cgcaaggcgt cgccgatcgc cgctccggtg acgtcccgcg
     3541 agcgcagtgc catggcgaat tcgctcgccc actgcttcgg gagggtcgtc ttcgtggggt
     3601 ccaacatggt ggttcctttc cggggtgggg agatcagccc gccggcacgg ttgtggcagg
     3661 ggagggtgca ggtatgccgg gtgcctccgc gcgcaggaag ctcgccgtgg tctcggcgaa
     3721 cgcgcaccag gaggcggccg ctgactccag gtgggagcgc ccggagtcgg tgatcgtgta
     3781 gtacttgcgg cccggcccgc cgtcgccggc ctcccatcgg gtctcgacgt gggcggcctg
     3841 ctcgaggcgg gcgagcaggg gatagagcgt gccgcccttg acggtgccca acccgttgcg
     3901 gtccaacgcg acagagatgg cgtaaccgta ggacgacggc tcgttggcca gcacccgaag
     3961 gaccgccatc tccagcacgc cccg
//
LOCUS       DAORPW010000220         3965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7160_length_3965_cov_650.322762, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000220 DAORPW010000000
VERSION     DAORPW010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7160_length_3965_cov_650.322762"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLB86_12185"
     CDS             complement(<1..254)
                     /locus_tag="PLB86_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC65946.1"
                     /translation="MSERATYHHGDLRQALISAAVDLISAKGVAGLSVAEAARKAGVS
                     GAAPYRHFASRTALLSAAATALATDLLEHLQVTAAGGPARG"
     gene            367..1215
                     /locus_tag="PLB86_12190"
     CDS             367..1215
                     /locus_tag="PLB86_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRC65947.1"
                     /translation="MHNVILNNGVEMPILGFGVFQIPDDETQAAVEAALAAGYRHLDT
                     AASYGNEEAVGRAIVASGIARSDIFVTTKLWIQSNPTQTKARAALEKSLERLGLDAVD
                     LYLIHQPFGDYYGSWRAMQTALGEGLARAIGVSNFQPDRLVDLIEHNEIAPAVNQIET
                     HPFFQRQIDQDVMRERGVQIESWGPFAEGRNGLFTNPELSEIAAARGKSVAQVVLRWL
                     IQREVVVIPKSVRPERLAENLDVFGFELTADDMSRIATLDTGASVFFDHRDPAMVSWL
                     NGRREA"
     gene            1249..2142
                     /locus_tag="PLB86_12195"
     CDS             1249..2142
                     /locus_tag="PLB86_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HRC65948.1"
                     /translation="MPPAPGSPAPGPSAPDRRRTQSTPAPAGVLGVLVGGAALLVTLA
                     ALVAWLSPATLGERPATCLTTSGARCFCEAVGTGLLRQPANSLSSLGFCVAAVTTAAR
                     GRRLGRHTPERVLAPVVALTLVALGAGSLAYHAQLTFAGQVLDVQGMYLLGTLLVVGA
                     LWRRGTLDATRAAAWAAALLLTLLLAQAILPDARRWLFALVLLPGIVLEWRLAPRSRP
                     LAIAVLALGIAYAAWLADDRGLWCEPGSWLQGHALWHLLTALAGGLLVAHYGRTASGH
                     QNAPGANAGGETELPRVVGQT"
     gene            2393..3334
                     /gene="folP"
                     /locus_tag="PLB86_12200"
     CDS             2393..3334
                     /gene="folP"
                     /locus_tag="PLB86_12200"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRC65949.1"
                     /translation="MSTEPPGNTPAPLVLRGQTFDAARPAVMAIVNRTTDSFYVGNRH
                     ADLDSALAALDSAVALGADIVDIGGVRAGQEGEAVTPEMEIDRVMPFLERARAAYPDL
                     ILSLDTWRSEVARAAAHAGVDLVNDTWAGYDPDLLATAVEIGAGYVISHTGGYPPRTD
                     PVDVTYGPGELDVVDDVVSTLRSGAARAVAAGMPRERVLVDPTLDFGKTTTHSLRVLR
                     HTEAVTALGFPVLQALSRKDFVGETLDLPADERLEGSLAATAVAAWLGATVFRAHDVG
                     ATRRVVDMVASIRGDRPPLVSLRGIGPHSRPADGATP"
     gene            3653..3856
                     /locus_tag="PLB86_12205"
     CDS             3653..3856
                     /locus_tag="PLB86_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRC65950.1"
                     /translation="MAQGVVKWFNAEKGFGFIAQDGGGPDVFVHYSAIQTQGYRSLDE
                     AQRVEFEVTQGPKGPQADAVRPL"
BASE COUNT          608 a         1404 c         1309 g          644 t
ORIGIN      
        1 ccacgggcag gacccccggc agccgtcact tgcagatgct ccagcaggtc cgtcgccaac
       61 gcggtcgcgg ctgccgacag cagcgcggtg cggctggcaa agtgccggta cggggcggcc
      121 ccgctcaccc ctgccttgcg cgcagcctcc gcaacggaga ggccagcgac gcccttcgcc
      181 gagatcaggt cgacggcagc ggagatgagc gcctgacgaa gatcgccatg gtggtaggtc
      241 gctcgctcgc tcacgtcacc tcccgcacag cccggttgac catcaatgta agagtctctt
      301 acattagacc ctgcacgcaa ggtggcacgc gaccaccaca cccgaagatc cggcgagagg
      361 cctgccatgc acaacgtcat cctgaacaac ggcgtcgaga tgccgatcct cggcttcggc
      421 gtcttccaga tccccgacga cgagacccag gcggcggtgg aggccgcgct cgccgcgggc
      481 taccgacacc tcgacacggc ggcgtcttac gggaacgagg aggccgtggg gcgggcgatc
      541 gtggccagcg gcatcgcgcg atcggacatc ttcgtcacga cgaagttgtg gatccagagc
      601 aacccgaccc agaccaaagc ccgcgcggcc ctcgagaagt cgctcgaacg cctcggcctc
      661 gacgctgtcg acctctacct catccaccag ccgttcggtg actactacgg ctcgtggcgg
      721 gccatgcaga ccgcccttgg tgagggtctg gctcgcgcga tcggcgtctc caacttccag
      781 cccgaccggc tcgtcgacct catcgagcac aacgagatcg ctccggcggt caaccagatc
      841 gagacccacc cgttcttcca gcgtcagatc gaccaggacg tcatgcgcga gcgcggcgtg
      901 cagattgagt cgtggggacc attcgccgag ggccgcaacg gcctcttcac caaccccgag
      961 ttgagcgaga tcgcggcggc ccgcggcaag tcggtcgctc aagtcgtgct ccgttggctg
     1021 atccagcgcg aggtcgtcgt cattcccaag tcggtgcgcc ccgagcgact ggccgagaac
     1081 ctcgacgtct tcggcttcga gctcaccgcc gacgacatga gccggatcgc cacgctcgac
     1141 accggcgcgt ccgtcttctt tgaccaccgt gaccccgcga tggtcagctg gctcaacggt
     1201 cgccgcgagg cctaactcct cgcgcggtca tgcgacagac tcctgggtat gccgcccgcg
     1261 ccaggatcgc ccgcgccagg accgtcggca cctgaccgtc gtcgcacgca gagcacaccc
     1321 gccccggcgg gcgtcctcgg cgtcctcgtc ggcggcgccg cgctactcgt caccctggcc
     1381 gcgctcgttg cgtggctctc cccggcgacg ctcggcgagc gaccggcgac ctgcctcacc
     1441 acgtcgggcg cgcgctgctt ctgcgaggcc gtcggcacag gcctgctgcg ccaaccggcc
     1501 aattccctct ccagccttgg cttctgcgtc gcagccgtca cgacggccgc gcgcggaagg
     1561 cgactggggc ggcatacccc tgagcgggtc ctcgcccccg ttgtcgcgct gaccctcgtc
     1621 gcgctcgggg cgggcagtct ggcctatcac gcgcagttga ccttcgccgg gcaggtgctc
     1681 gacgtgcagg gcatgtacct cctgggaacc ctgctcgtcg tcggtgccct ctggcggcgt
     1741 ggcaccctcg acgccacccg cgcggcagcc tgggcggcgg ccctcctgct cacgcttctg
     1801 ctcgcgcaag cgatcctgcc ggacgcgcgg cgctggctgt tcgcgctcgt cctgctcccc
     1861 gggatcgtcc tcgaatggcg gctggccccc cgctctcgcc ccctcgccat cgcggtgctc
     1921 gccctcggca tcgcgtatgc cgcgtggctg gccgacgatc ggggtctgtg gtgtgagccg
     1981 ggctcctggc tgcagggcca cgccctctgg catctgctca ccgcgcttgc aggtgggctg
     2041 ctcgtcgccc actacggccg aactgcgtca ggacaccaaa acgcccccgg cgccaatgcc
     2101 gggggcgaaa cggaactgcc gcgagttgtg ggtcagacct gacgtcccat gaccatgtca
     2161 cgacggtcgt agtggtaggt ccccgcgtgc gaggtggtgc ggccaaacat cggaaggtag
     2221 aaaatccagc tgggcatcgt gtgaacttcg tttcgtatca atgggttacg cgcctccata
     2281 tcggagactc gttgtctatc ataccatttc gagcatgctc ctggccaatc ggcggagcat
     2341 gccgaagcac acgcggaccc tggggaatac cctggcgtgc gacgatgacg ccgtgagcac
     2401 cgagcctccc ggcaacaccc ccgcacctct cgtcctgcgc ggccagacct tcgacgcggc
     2461 ccgtcccgcg gtgatggcca tcgtcaaccg gacgaccgat tctttctacg tgggcaaccg
     2521 gcacgccgac ctcgacagcg ctctggcggc cctcgactct gcggtcgcct tgggcgctga
     2581 catcgtcgac atcggcgggg tccgcgccgg gcaggagggc gaagcggtca cgcctgagat
     2641 ggagatcgat cgggtgatgc cgttcctcga acgggcgcgc gcggcatacc ccgatctcat
     2701 cctctccctc gacacctggc ggtccgaggt ggcccgtgcg gcggcccacg cgggggtcga
     2761 cctggtcaac gacacctggg cgggctacga cccggacctg ttggcgacgg cggtggagat
     2821 cggtgccgga tacgtcatct cgcacacggg cggctacccg ccgcgcaccg atccggtgga
     2881 cgtcacctac ggtcccggcg agctcgacgt ggtcgacgat gtcgtgtcga cgctgcggtc
     2941 gggcgcggcg cgggccgtcg ctgccggtat gccgcgcgag cgggtcctcg tcgaccccac
     3001 cttggacttc ggcaagacga cgactcactc gctgagggtg ctgcggcata ccgaggcggt
     3061 gacggccctg ggcttcccgg tgttgcaggc gttgtcccgc aaggacttcg tcggcgagac
     3121 cctcgacctg ccggccgacg aacgcctcga aggcagcctg gccgcaacgg ccgtcgcggc
     3181 gtggctcggg gcgacggtat tccgcgccca cgacgtcggc gcgacccgtc gggtggtcga
     3241 catggtggcc tcgatccgag gcgatcggcc gcccctcgtg agcctgcgcg gtatcggccc
     3301 gcacagccgt ccggcggacg gcgcaacgcc ctgagcgatg cacccaaaaa ggttctgacg
     3361 cagacctttt cgtaggtcgg gggaggggcg ctggtcgacc cgtgagacta cccgtcggta
     3421 gcccaaaaaa atggtgggtc tgagcaggtg tagtgccgat cactgcgtgc taacgtcggc
     3481 gccggttgca gttgcagttc ctcgcaagac ggacacgacg cccatcgcct ttgggcagcg
     3541 gacgtcgtgt tcaagcactc gggcgcaggc tcgcgagcgg tgggttgtgg cagcagcacg
     3601 ggagttcgtg aggtgcgggc tccagtgtcc agttatagag agagttgtcc ccatggcaca
     3661 gggagtcgtc aagtggttca acgctgagaa gggctttggt ttcatcgccc aggacggcgg
     3721 cggccccgac gttttcgttc actactcagc aatccagact cagggttacc gctcgctcga
     3781 cgaggcacag cgcgtcgagt tcgaagtgac ccagggcccc aagggcccgc aggcggacgc
     3841 cgtccgcccg ctgtaatcac gcgagttcct cgcagctgac cccggtcagc gacacgagcc
     3901 ccccggacgt cggtccgggg ggcacgtgtg ttctcaggct ggaagatcac cggttcctgc
     3961 aggta
//
LOCUS       DAORPW010000221         3958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7178_length_3958_cov_756.399180, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000221 DAORPW010000000
VERSION     DAORPW010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7178_length_3958_cov_756.399180"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..848)
                     /locus_tag="PLB86_12210"
     CDS             complement(297..848)
                     /locus_tag="PLB86_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65951.1"
                     /translation="MTPLSWDPTPGAHIYVDESTAGDYLLMGAAIASADAPQMRSVMR
                     GLLLPGSRSLHMRKEQKRAATILGTVVGLGPNVAIFRVSRTTPALDARRATVHALAEL
                     ACQLRADRVVFDTIDSMSARDRAWVIEGVHLAGEPTPPFGYHHQRRHEEPLLWIPDAV
                     GWAWARGGQHRRAVERICYLVDL"
     gene            complement(923..1645)
                     /locus_tag="PLB86_12215"
     CDS             complement(923..1645)
                     /locus_tag="PLB86_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65952.1"
                     /translation="MAMLRGVLRVSPIGDYRTLPALLGVDFLVVDEPGGHGSGPFHPI
                     VEVEDLAALYEMLRTERIVVGVLTPLDDGQRHSFTVRDLRGMALLTVVGDLPPTHRLL
                     TGPDVRAHVRLPFDPRTNVLDPCTWVLAEVGRKLTGESVVGIDMQPVDRDRRAVFVQD
                     QRGRRVLVGEVRVMRGSEYLDMDVTLDGGLVTNVVVEVVHDASDAMVSIVHSGWVVGS
                     EEQRGQFDGWQDALSAWAASQT"
     gene            1850..2461
                     /locus_tag="PLB86_12220"
     CDS             1850..2461
                     /locus_tag="PLB86_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC65953.1"
                     /translation="MADVKKGPSPVVSTASFEAELNALLLRAGHTVPGAVVAAALKAV
                     ERRRSLARIGPEATTSSRTEQDEFLKASASVDVAPDQGAAMLRLDLSRREEVMLTTAQ
                     VAKDLGKAKSTITRQVNRGDLYAIKGDGQLRLPAWQFVSGAAVPGLAQALAYVPETWG
                     PRRLRLFMTTPEEALGDRSPVQWLLDNEPPQVVAQLMADESRE"
     gene            2458..3111
                     /locus_tag="PLB86_12225"
     CDS             2458..3111
                     /locus_tag="PLB86_12225"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HRC65954.1"
                     /translation="MTPKPQPKTPRKPPKPLTRRSSDLVGVRPTLWRIGATIGDYVLE
                     WNEARDFGPLRTMRWDPHHPPQSVQPDRAVIYASPSPITAVAEKFQKYRTIDPFTATP
                     CLYGWSATRQLTLLDLRGNWPINNKGSASMPMGPRNVCRAWSAAILDTWPELDGLLTR
                     STMDGRDTYVLFPPARTSMPDEPRHARLLTDPAVYCYLDLWADEINYDIADAPWPPA"
     gene            complement(3149..3913)
                     /locus_tag="PLB86_12230"
     CDS             complement(3149..3913)
                     /locus_tag="PLB86_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC65955.1"
                     /translation="MLLKDKIAVITAGASGMGQASAQLFAEHGAHVIVVDVNEGAATA
                     TVEGIIAAGGSAEAAAVDVRDLDALERLGAKITADHGKVHILYNNVGIPGAAGMQLSE
                     ADWDTGIEINARASFFLTGHLLDALKAADGASVIFTSSTSGLVGSPFSPLYSFTKGGI
                     IALVRSYALALAADNVRVNAIAPGQVETPGLAAFFRIDDPEEIARRKAAFAATIPLGR
                     ASDPREIASVALFLASDLSSYVTGVTIPVDGGITAK"
BASE COUNT          701 a         1354 c         1259 g          644 t
ORIGIN      
        1 cgcccaagga gtcatcgacg gcctgtcctg agttgaccgt cggcttgcga tgccccatgt
       61 ctgccctcgc cgcatcccat cgtttccctt cgcgcgtcgc ggcatccacg tggggggctg
      121 tcacaacgga gtacctcagg tacgcagtgc gtacctgagg tacgtgatcc ctgcaggggt
      181 cctgatcaca acagctaggg caaagcggct atgcttgaag ttacgaaggg gaattacccc
      241 ccaagtagtg agcccgatac ccgtccggac ggtcgcggtg tcgggcttcg cgctgtctac
      301 aggtcgacca ggtagcagat tcgctcgacc gcgcgacgat gctgaccccc tctggcccac
      361 gcccagccga ccgcatcggg tatccagagc aggggctcct cgtgacggcg ctggtggtga
      421 tagccgaacg ggggagttgg ctcgccggcg aggtgaactc cttcgatgac ccacgctcgg
      481 tcacgggcgg acatggagtc gatggtgtcg aagacgacac ggtcagcgcg aagttggcat
      541 gccaactcag caagggcgtg gacggtggca cgtcgggcgt cgagggccgg agtggttctg
      601 gagactcgga agatggccac gttggggccg aggccgacga cggtgcccag aatggtggcg
      661 gcgcgtttct gttcctttcg catgtggagg ctgcggctgc cgggcaggag cagccctcgc
      721 atgactgagc gcatctgtgg ggcgtcggcg ctcgcgatcg cggcgcccat caggaggtag
      781 tccccggccg tggactcgtc gacatagatg tgcgctccgg gcgtgggatc ccacgagagg
      841 ggagtcaccg ttcttgcacg cctatgcgcg gggcttcccc ccaaaaagcg gtgctgggtc
      901 ccgcgagagt cccgagaacg cttcaggttt ggctggctgc ccaggcactc agcgcgtcct
      961 gccacccgtc gaactgaccc cgctgctctt cggagcccac gacccagccg ctgtgaacga
     1021 tgctgaccat ggcgtcggag gcgtcgtgga ctacctcgac gaccacgttg gtcaccagac
     1081 ccccgtcgag agtgacgtcc atgtccagat actcgctacc gcgcatcacg cgcacttcgc
     1141 cgacgagtac acgccggcca cgttggtcct gcacgaacac agcgcgcctg tctcggtcga
     1201 cgggctgcat gtcgatcccc acaacggact ccccggtcag cttcctgccc acctcggcga
     1261 ggacccatgt gcaaggatcc agaacgttcg tgcgtgggtc aaaagggagc cgaacatgcg
     1321 cgcgcacatc gggtcccgtc agcaacctgt gcgtgggcgg caggtctccc accaccgtaa
     1381 ggagagccat cccgcgcagg tcgcggaccg tgaacgaatg acgctgaccg tcgtcgaggg
     1441 gtgtcagcac cccaacaacg atccgttccg ttcgcagcat ctcgtacagc gcagcgagat
     1501 cctccacctc aacaatcggg tggaaagggc cggagccatg ccctcctggc tcgtcgacca
     1561 cgaggaagtc gacgcccaac aacgcgggca aggtgcggta atccccgatc gggctgaccc
     1621 gtagaacccc cctcagcatc gccatcccga ttccctccgt ctcactgggc cccggcatga
     1681 cgtgcgcccg aagctaacta gttggttgcg acgatacggc ggcaggctga caaccgccgt
     1741 cggccggcac cgacggatcg ccacactcct cgcatcggtc gcggaggccc tcgatgcatc
     1801 gctttgcacc tcgcatgcgt tccgatgcgt aaagcgctag aatagctcca tggctgacgt
     1861 caagaaggga ccgtctccgg tcgtatcgac agcctcgttt gaggcagaac tgaatgccct
     1921 cctgctcagg gccgggcaca cggtgcccgg ggcagtggtc gccgcagccc ttaaggccgt
     1981 ggaacgtcgc cgaagccttg cgcggattgg tcctgaagcg accacctcaa gccgaacgga
     2041 gcaggacgag ttcctcaaag cgagcgcgag cgtggacgtt gcgccagatc agggcgcggc
     2101 gatgcttcgg ctcgacctat cccgccgcga ggaggtcatg ctcacgacag cgcaggtggc
     2161 caaggacctc gggaaggcga agtcgacgat cactcgccag gtcaaccggg gtgacctcta
     2221 cgcaatcaag ggtgatgggc agctgcggtt gccggcctgg cagttcgtgt ccggggctgc
     2281 agtccccgga ctcgcccagg cactggccta cgtccccgag acctggggac ctcgacggct
     2341 tcggcttttc atgacaacgc ctgaggaagc actgggcgac cgcagccccg ttcagtggct
     2401 gctggataac gagcccccac aggtggtcgc ccaactgatg gccgacgaga gccgcgaatg
     2461 acgcccaagc cccaaccgaa gaccccccga aagccgccga aaccgttaac ccgccgatcc
     2521 agcgacctcg tcggggtccg gcccacgctc tggcgcattg gggcgaccat cggggactac
     2581 gtgttggagt ggaacgaagc ccgagacttc gggcccttgc ggacgatgcg gtgggacccg
     2641 caccacccac cccaatccgt acaaccggac cgcgccgtca tctacgcttc accgtcaccg
     2701 atcaccgccg tggctgaaaa gttccagaag taccgcacca tcgacccctt caccgcaacc
     2761 ccgtgcctct acgggtggtc ggcaacacgg caattgaccc tcctggacct gcggggcaac
     2821 tggccgatca acaacaaggg atccgcgtcg atgccgatgg gaccgaggaa cgtttgccgg
     2881 gcttggtccg ccgcgatcct cgacacctgg cccgagctcg acgggctcct gacacgatcc
     2941 acgatggacg gccgcgacac ttacgtcctt tttcctccgg cgcgcacctc gatgcccgac
     3001 gaacctcgcc acgcgcgact gctgaccgac ccggccgtct actgctacct cgatctgtgg
     3061 gccgacgaga tcaactacga cattgccgac gcaccctggc cacctgcctg aggggactgc
     3121 caaaacgggc tggccgctgc gccatcacct acttcgcggt gatgccgccg tcgacgggga
     3181 tcgtcacccc ggtgacgtaa ctggacagat cgctcgccag gaacagcgcg accgacgcga
     3241 tctcacgcgg gtcgctggcc cggcccaacg ggatggtcgc agcgaacgcg gccttgcgcc
     3301 gggcgatctc ttccgggtcg tcgatccgga agaacgcggc cagccccggc gtctcgacct
     3361 gacccggagc gatcgcgttg acccgcacat tgtcggccgc cagcgccagc gcgtaggagc
     3421 gcaccagcgc gatgatcccg cccttggtga acgaatagag cgggctgaac ggcgacccga
     3481 ccaagcccga cgtcgaactc gtgaagatga ccgacgcgcc gtcggccgcc ttgagcgcat
     3541 cgagcaggtg ccccgtcagg aagaagcttg cccgcgcgtt gatctcgatg cccgtgtccc
     3601 aatcggcttc cgagagttgc attcccgcgg ctccggggat cccgacgttg ttgtagagga
     3661 tgtgcacctt gccgtggtcg gcggtgatct tggcgccgag ccgctccaac gcgtcgaggt
     3721 cgcggacgtc gacggccgct gcctctgcgc tgccgcccgc cgcaatgatc ccctcgacgg
     3781 tcgccgtcgc cgcgccttcg ttgacgtcga cgacgatgac gtgcgcgccg tgctcggcga
     3841 acaactgcgc cgacgcctgc cccatcccgg acgccccggc ggtgatgacg gcgatcttgt
     3901 ccttgagcaa catggtggtc tcctcggtgg tcgggctggt caggcagtag gtctagtc
//
LOCUS       DAORPW010000222         3928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7287_length_3928_cov_791.048283, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000222 DAORPW010000000
VERSION     DAORPW010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7287_length_3928_cov_791.048283"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLB86_12235"
     CDS             <1..552
                     /locus_tag="PLB86_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRC65956.1"
                     /translation="RNVRPTIMIGTSTMGGAFTKAIVKDMAAHCERPIIMPLSNPTSR
                     AEATPADLVEWTDGHALIATGSPFDPVDHREVRHFIAQANNALIFPGLGLGVAACQAT
                     RVTDRMIAAAAEALAARVNTYRPGAALLPSMSELRPVSSHVALAVAAAAAEEGVARQP
                     LTDPINEIYSRMWQPAYSPVEVV"
     gene            complement(574..912)
                     /locus_tag="PLB86_12240"
     CDS             complement(574..912)
                     /locus_tag="PLB86_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRC65957.1"
                     /translation="MKLVTAVVKPHKWDEVRAALETVGVAGMTVSEVSGSGRQKGHTE
                     VYRGAEYDVTLVPKVRIEVLVDDHDVDDIVRTIVSAARTGRIGDGKVWVVPVDSVVRV
                     RTGERDEDAL"
     gene            complement(914..2227)
                     /locus_tag="PLB86_12245"
     CDS             complement(914..2227)
                     /locus_tag="PLB86_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRC65958.1"
                     /translation="MDTGDTAWLLVSSALVLLMAPGLALFYGGMVRSKSVLNMMMMTF
                     GALAVILVLWTLVGYSLAFGDDIGGGVLGNPLQYAGLGQLLSPEADVVGTVPAILFAV
                     FQGLFCVITGALVSGAIADRARFGAWLVFIGAWTVLVYAPVAHWVFDFSAGDHVGGWL
                     ANQVGVIDFAGGTAVEICSGASGLALALVLGKRIGFGRDPMRPHNLTLVMLGAGLLWF
                     GWFGFNAGSALGAGHKAAVVFVTTLLAGAAGTLGWLVLERFRDGKATSLGAASGMVAG
                     LVAITPSGGSLTPIGALLMGGVAGVVCAAAVGLKFRFGYDDSLDVVGVHLVGGLIGTL
                     GIGLLGTSAAPTAVDGLFYGGGVTQLGKQAIGAAVVLVYAFVVSAALGYAVERLIGFR
                     LIPEHEVSGIDLAIHGETAYDFHTVTGGRAGGHGILRSLSHPEED"
     gene            2655..>3928
                     /locus_tag="PLB86_12250"
     CDS             2655..>3928
                     /locus_tag="PLB86_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC65959.1"
                     /translation="MTLSIATGETDLPLLHETISANLDATAARFPEHEALVDCATGKR
                     WTYAAFAADVDTLARGFAGSGVVKGDRVGIWAPNGAEWTLVQYATAKVGAILVNINPA
                     YRTHELQYVLNQAAIKVLVAAPSFKTSDYAGMIDEVRDEVATLEQVVLIGRESWDELW
                     AAAEQVSQAELEAIAAGLSPDDPINIQYTSGTTGFPKGATLSHVNILNNGFHVGEVCR
                     YTEADRICIPVPFYHCFGMVMGNLAATTHGACMVIPAPGFDPALTLAAVRDERCTSLY
                     GVPTMFVAEWALIEAGAASESDLATVRTGIMAGAPCPAELMKKVIAAGINEMTICYGM
                     TETSPVSTQTRTDDSFERKVGTVGRCMPHLQIGIIDPSDGSFVERGGSGEFVTKGYSV
                     MLGYWNQPDKTAESIVDGWMRTGDLAVMDEDG"
BASE COUNT          668 a         1371 c         1341 g          548 t
ORIGIN      
        1 cgcaacgtcc gcccgacgat catgatcggg acctcgacga tgggcggggc cttcaccaag
       61 gccatcgtca aggacatggc ggcgcactgc gaacggccga tcatcatgcc gctgtccaac
      121 cccacttccc gcgccgaggc cacgcccgcc gacctcgttg agtggaccga cggccacgcc
      181 ctcatcgcca ccggcagccc cttcgacccg gtcgaccacc gcgaggtgcg ccacttcatc
      241 gcccaggcga acaacgccct gatcttccct gggctcgggc tcggggtcgc cgcgtgccaa
      301 gcgacccggg tgaccgaccg gatgatcgcg gccgccgcgg aggctctcgc cgcgcgggtc
      361 aacacctatc gcccgggtgc cgcgttgctg ccgtcgatga gtgagcttcg gccggtctcc
      421 tcgcacgtcg cgctcgccgt ggccgccgct gccgccgagg agggcgtcgc ccgccaaccc
      481 ctcaccgacc cgatcaacga gatctacagc cgcatgtggc agccggccta ctccccggtc
      541 gaggtcgtct gagttcggca gtccgacgcg cctctagagc gcgtcctcgt cgcgctcgcc
      601 ggtgcgcacc cgcacgacgc tgtcgacggg cacgacccac accttgccgt cgccgatccg
      661 gccggtgcgt gcggcgctga cgatggtgcg gacgatgtcg tccacgtcgt ggtcgtcgac
      721 gagcacctcg atgcgcacct tcggcacgag cgtgacgtca tactccgcgc cgcgatagac
      781 ctcggtgtgg cccttctgcc ggcccgaccc gctcacctcc gacacggtca tcccggccac
      841 gccgacggtt tcgagggcgg cgcgcacctc gtcccacttg tgcggcttga ccaccgcggt
      901 gacgagcttc acgtcagtcc tcctctggat gggacagcga gcggagtatg ccgtgtccac
      961 cggcgcgccc gcccgtcacg gtatggaagt cgtaggcggt ctcgccatgg atggccaaat
     1021 cgatgccgct cacctcgtgc tcggggatga gccggaagcc gatgaggcgc tccacggcat
     1081 accccagggc tgcggagacg acgaaggcgt agacgaggac gacggcggcg ccgatcgcct
     1141 gcttgcccag ttgggtgacc ccgccgccat agaacagccc gtcgacggcg gtgggggcgg
     1201 ccgaggtgcc gagcaggcca atgcccagag tgccgatgag gccaccgacg aggtggacac
     1261 cgacgacatc gagcgagtcg tcgtagccga agcggaactt cagcccgacg gccgccgcgc
     1321 agacaacgcc ggccacgccg cccatgagga gcgcgccgat cggggtgagg gagccacccg
     1381 acggggtgat cgccacgagc ccggcgacca tgccggacgc ggcgccgagc gaggtcgcct
     1441 tgccgtcgcg gaagcgttcg agcacgagcc agccgagggt gcccgccgct ccggccagca
     1501 gcgtcgtcac gaacacgacg gcggccttgt ggccggcccc cagcgccgag ccggcgttga
     1561 agccgaacca gccgaaccac agcagcccgg cgccgagcat gacgagggtg aggttgtgcg
     1621 ggcgcatcgg gtcgcggccg aagccgatcc gcttgccgag caccagagcg agcgccaatc
     1681 cggaagcgcc cgaacagatc tcgactgccg tgccgccggc gaagtcgatg accccgacct
     1741 ggttggccag ccagccgccg acatggtccc cggccgagaa gtcgaacacc caatgggcca
     1801 ctggcgcata gacgagtacc gtccaggcgc cgatgaagac cagccaggca ccgaaccgcg
     1861 cgcggtcggc gatcgccccg gagacgaggg cgccggtgat gacacagaac aagccctgga
     1921 agaccgcgaa gaggatcgcg gggacggtgc ccacgacgtc agcctcgggg gagagcagtt
     1981 ggccgagacc ggcatactgc agcgggttgc cgagcacccc cccaccgatg tcgtccccga
     2041 aggcgagtga gtagcccacg agcgtccaga ggacgaggat gaccgccagc gcgccgaagg
     2101 tcatcatcat catgttgagg acgctcttgg agcgcaccat cccgccgtag aacagggcca
     2161 ggcccggtgc catgagcagc acgagggcgc tggagaccag caaccaggcg gtgtcgccgg
     2221 tgtcgatctc ggtgacgagt gtggtgaagg cgggcaacgt gctcacggcg gtcccccttg
     2281 cgggcagggg cgggttgggc gcgcccacgg tagcgcgctc caggtatgcc gctcagtccc
     2341 gtccgcgccg gggcctcggt ccgaatctgg ttcgccgcga cggtcccagg cctggggagt
     2401 gcgccacgga gtgcgatcgg cggggtgatc accccgtcca acgcactccg tcgggccctc
     2461 gcttagcccg ggcagcgtgc ctggcgctgc ggattcccga acagccggta gatgacttac
     2521 gcagacaacg tcggcagcga cgcggcatac ctgcgccgta tgccgccatg cggcaaaggt
     2581 gaccatagtc gggttgcgtt tggctccctt gggcgatgga ctgccctgga cacctcggac
     2641 gaaaggccgc tcccatgacc ctgtcgatcg cgaccggcga aaccgacctc ccgctcctgc
     2701 acgagacgat ctcggccaac ctcgacgcca cggccgcccg cttccccgaa cacgaggcgt
     2761 tggtcgactg cgcaacgggc aagcgctgga cgtatgccgc gttcgccgcc gacgtcgaca
     2821 cgttggcccg cgggttcgcc ggcagtgggg tcgtcaaggg cgaccgggtc gggatctggg
     2881 cgcccaacgg cgccgagtgg acgctcgtgc aatacgcaac ggccaaggtc ggcgcgatcc
     2941 tcgtcaacat caacccggcc tatcggaccc acgagttgca gtacgtcctc aaccaggcgg
     3001 cgatcaaggt ccttgtcgcg gctccgtcgt tcaagacgag cgactatgcg gggatgatcg
     3061 acgaggtccg cgacgaggtg gcgaccctcg agcaggtcgt cctcatcggg cgcgagtcgt
     3121 gggacgagtt gtgggcggca gccgagcagg tcagccaggc ggagttggag gcgatcgcgg
     3181 cggggctctc gcccgacgac ccgatcaaca tccaatacac ctcgggcacc acgggattcc
     3241 ccaagggtgc gacgctcagc cacgtcaaca tcctcaacaa cgggttccat gtgggcgagg
     3301 tctgccgcta caccgaggcc gaccggatct gcattcccgt gcccttctat cactgcttcg
     3361 gcatggtcat gggcaacctc gcggcgacca cccatggggc gtgcatggtc atcccggcgc
     3421 ccggcttcga cccggcgctc accctcgccg cggtccgtga cgagcgatgc accagcctct
     3481 atggcgtgcc gacgatgttc gtcgccgaat gggcgctcat cgaggccggt gccgcgagcg
     3541 agagcgacct ggcgaccgtg cgcaccggga tcatggcggg ggcgccgtgc ccggccgagt
     3601 tgatgaagaa ggtcatcgcc gcaggcatca acgagatgac gatctgctac ggaatgaccg
     3661 agacctcgcc ggtgtcgacc cagactcgca ccgacgactc gttcgagcgc aaggtgggga
     3721 ccgtcgggcg gtgtatgccg cacctgcaga tcgggatcat cgacccgagc gacgggtcgt
     3781 tcgtcgagcg cggcgggtcc ggtgagtttg tcaccaaggg ctattcagtg atgctcggct
     3841 attggaacca gcccgacaag accgccgagt cgatcgtcga cggatggatg cgcacgggcg
     3901 acctggcggt gatggacgag gacggcta
//
LOCUS       DAORPW010000223         3873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7455_length_3873_cov_938.012310, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000223 DAORPW010000000
VERSION     DAORPW010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7455_length_3873_cov_938.012310"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PLB86_12255"
     CDS             <1..174
                     /locus_tag="PLB86_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRC65960.1"
                     /translation="EAALLVEHAGWTLGDLRWVTINAMKSAFLPFDERLAFIDDTIKP
                     GYAALGASASAPR"
     gene            complement(232..1080)
                     /locus_tag="PLB86_12260"
     CDS             complement(232..1080)
                     /locus_tag="PLB86_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRC65961.1"
                     /translation="MSQPMIATIGVTEDDLVTGEAVALDLPPASVGLRILAGMLDLII
                     VQVLTYVVFVAGALLSNQLDGALGASILVLLTVACLVGYPTAMLTLTRGKTVGKLAAG
                     LRVVRDDAGPITFRHALTRSLVGVIEVFAFYGVPAVIASIINPRGKRLGDMAAGTYVV
                     RERTRASLPPPVPMPPHLAVWAASADIAQLPDRLAADVRTFLPAAFGMDPNAREELGR
                     ALLAEVMEFVSPLPPAGNHPEYILAAVVADRRRRDLGRLMRDATLRDRLIPPDPLVTP
                     PSGMPR"
     gene            1099..>2078
                     /locus_tag="PLB86_12265"
     CDS             1099..>2078
                     /locus_tag="PLB86_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HRC65962.1"
                     /translation="MDLDAYVAAHTHEWSRLEQLVGQRGKLQGAQADELLDLYQRTAT
                     HLSEIRSTAPEPSVVAYLSSMLARARSRAVGTRSTSWSDFGRFFVVTFPAALYRTRRW
                     WLTTMAINVVVGFAIGWWFVVNPQFESALASPAEIQQLVTHDFADYYSEYAAGSFALK
                     VWTNNAWVTALCISFGVLGLPVLWLLWNNILNVSVIGAIMWRYDRADLFFGLILPHGL
                     LELTAVFVAAGVGLRMFWAWIEPGPQTRMTAIAREGRAAMSVALGLVVVLFISGVIEA
                     FVTPSPLPTWARIGIGILAEAVFLTYVFTLGRYAVRRGETGDVAAVDQGDT"
     assembly_gap    2079..2178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2181..3476)
                     /locus_tag="PLB86_12270"
     CDS             complement(2181..3476)
                     /locus_tag="PLB86_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC65963.1"
                     /translation="MTVSGRLPILVLLGAVAVVLEPTRRTVLLWSLVCLALVLLDVLL
                     ALSPTRLVIEREPVPSVRLGESAHSGVLVTNPTRRTLHGHLRDAWVPSAGARGERHTA
                     RIKGGERRRFTTTLHPTRRGDRHADRVTVRSFGPLRLAARQRSFDVAGTIRALPPFES
                     RKHLPSRLASLRQLDGRSAVRTRGQGTEFDSLRDYVEGDDVRSIDWRATARRRNVVVR
                     TWQPERDRRVIIVLDTSRTSAGRVGDQPRLDAAMDAALLLAALASRAGDRVDFLAGDR
                     WLRARVAAAANRTTLLNDLVQAMAPLDPALLEASWPTLASAIADTSRRRALVVLLTPL
                     EPAAIQESLLPSLPALTRHHRVVIASVADPAIAALAETRETTKDAYAAAAAERTMALQ
                     DRTAAVLRRMGVAVIDASPAELPPKLADHYLMLKAQGLL"
     gene            complement(3496..>3873)
                     /locus_tag="PLB86_12275"
     CDS             complement(3496..>3873)
                     /locus_tag="PLB86_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRC65964.1"
                     /translation="DPRDLVAAGIRAVASAADLAAGAAEVRQVRIAPEVAAYIVDIGR
                     ATRQSPSLSLGVSPRGATALLATSRAWAWMNGRTFVTPDDVKALAHATLAHRLTLRPE
                     AELEGVGVAAVLATAIASVPVPR"
BASE COUNT          539 a         1278 c         1411 g          545 t
ORIGIN      
        1 gaggctgcgc tgctcgtcga gcacgccggg tggacgttgg gcgatctgcg ctgggtgacg
       61 atcaacgcga tgaagtcggc gttcctgccg ttcgacgagc ggctcgcgtt catcgacgac
      121 acgatcaagc cggggtatgc cgccctcggg gcctctgcgt cggcccctcg gtagcgtcgg
      181 cccctcggta ggagcggccc tcggttgggg ccggcccgcg ggtcgaacgg gtcagcgcgg
      241 catacctgat ggtggggtga ccagcgggtc cggcgggatg agtcggtcgc gcagggtggc
      301 gtcgcgcatc agccggccga ggtcgcggcg gcggcggtcg gcgaccacgg cggcgaggat
      361 gtattccggg tggttgcccg ccggcggcag gggcgagacg aactccatga cctcggcgag
      421 cagcgcgcgg cccaactcct cccgagcgtt cgggtccatc ccgaacgccg ccggaaggaa
      481 ggtccgcacg tcagcggcga gccggtcggg cagttgggcg atgtcggccg acgcggccca
      541 cacggccagg tgcggcggca tggggaccgg aggcggaagg ctcgcccggg tgcgctcacg
      601 cacgacgtag gtgccggcag ccatgtcacc caaacgcttg ccgcgcgggt tgatgatgga
      661 cgcgatgacg gcggggaccc cgtagaaggc gaacacctcg atgacgccga cgaggctgcg
      721 ggttaacgcg tgacggaagg tgatcggtcc cgcgtcgtca cgcaccaccc gcaggccggc
      781 ggccagtttg cccaccgtct tgccccgcgt cagggtgagc atggccgtcg ggtagccgac
      841 gaggcaggcc accgtgagca gcacgaggat cgaggcaccg agggcaccgt cgagttggtt
      901 ggacaacagg gcgccggcga cgaagacgac ataggtcagg acctggacga tgatcaggtc
      961 gagcatgccg gcgaggatcc gcagcccgac cgaggccggg ggcaggtcga gggcgaccgc
     1021 ctcaccggtg acgaggtcgt cctcggtgac accgatcgtc gcgatcattg gctggctcac
     1081 acggcataca gtaaccgtgt ggacctggat gcctacgtgg ccgcgcacac tcatgagtgg
     1141 tcgcggctcg agcaactggt cgggcaacga ggcaaactcc agggcgctca ggccgacgaa
     1201 ttgctcgacc tctaccagcg cacggccacc cacctgtccg agatccgctc caccgcgccg
     1261 gaaccctccg tcgtcgccta cctgtcctcg atgctcgccc gcgcccggtc ccgagccgtc
     1321 gggacccgct cgacgtcgtg gagcgacttc gggcgattct tcgtcgtgac cttcccggcg
     1381 gccctctatc gcacccgccg ctggtggctg acgacgatgg cgatcaacgt cgtcgtcggc
     1441 tttgccatcg ggtggtggtt cgtcgtcaac ccgcagttcg agtcagccct cgcctccccg
     1501 gcggagatcc agcaactcgt cacccatgac ttcgccgact actacagcga gtatgccgcc
     1561 ggctccttcg cattgaaggt gtggacaaac aacgcctggg tcaccgccct gtgcatctcg
     1621 ttcggcgtct tggggctgcc ggtcctctgg ctgctgtgga acaacatcct caacgtctcc
     1681 gtcatcggcg cgatcatgtg gcgctacgac cgggctgacc tgttcttcgg cctgatcctg
     1741 ccgcacggcc tgctcgagct caccgcggtg ttcgtcgcgg cgggcgttgg gctgcgcatg
     1801 ttctgggcct ggatcgagcc ggggccgcag acccggatga cggccatcgc ccgcgaaggt
     1861 cgagcggcca tgtccgtcgc gctcggcttg gtcgtcgtgt tgttcatctc cggcgtcatc
     1921 gaggccttcg tcaccccctc gccgttgccg acctgggccc ggatcggcat cggcatcctc
     1981 gcggaggcgg tgttcctcac ctacgtcttc accctcggcc ggtatgccgt ccgccgcggt
     2041 gagacgggcg acgtcgccgc ggtggaccag ggcgacacnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnngt ctagagcagg ccctgcgcct tgagcatgag gtagtggtcg
     2221 gcgagtttgg gcggcaactc ggccgggctg gcgtcgatga cggccacgcc catcctgcgt
     2281 agcacggctg cggtgcggtc ctgcagcgcc atggtgcgtt cggctgcggc cgcggcatac
     2341 gcgtccttgg tggtctctcg ggtctcggcg agcgcggcga tggccgggtc ggcgaccgac
     2401 gcgatgacga cccggtgatg gcgcgtgagg gccgggaggc tgggcagcag tgactcctgg
     2461 atcgccgccg gctcgagggg ggtgaggagc acgacgaggg cgcggcgacg actggtgtcg
     2521 gcgatggcgc tggccagggt gggccacgag gcttccagga gagcgggatc cagcggcgcc
     2581 atcgcctgaa cgaggtcgtt gagcagcgtc gtgcggttgg cggccgctgc gacgcgggcc
     2641 cgcagccacc ggtcgccggc gaggaagtcg acccggtcgc cggcgcggga agccagcgcg
     2701 gcgagcagca gcgcggcatc catcgcggcg tccagacgag gctggtcacc gacccggccg
     2761 gccgaggtcc gggaggtgtc caggacgatg atcacccgcc ggtcgcgctc gggctgccag
     2821 gtgcgcacga cgacattgcg ccgccgcgcc gtggcgcgcc agtcgatcga gcgcacgtcg
     2881 tcgccttcga cgtagtcgcg cagtgagtcg aactccgtcc cctgtcctcg ggtccgaacc
     2941 gccgagcgcc catccaactg gcgcaacgag gccaggcggg agggcaggtg tttgcgcgac
     3001 tcgaacggcg gcaacgcccg gatcgtcccg gcgacgtcga acgatcgctg cctggcggcc
     3061 agccgcagcg gcccgaacga gcggaccgtc acccggtcgg cgtgccggtc gccccggcgg
     3121 gtcgggtgca gcgtcgtcgt gaaccggcgg cgctcgcccc ccttgatccg cgcggtatgc
     3181 cgctcgcccc gggccccggc cgagggcacc caggcgtcgc gcaggtgccc atgcagggtg
     3241 cgtcgggtgg ggttggtgac gaggaccccc gagtgggccg actcgccgag ccgcaccgag
     3301 ggaacaggtt cgcgttcgat gaccaaccgg gtcggtgaca gggccagcag gacgtccagg
     3361 agcacgaggg cgaggcagac gagcgaccag aggagcacgg tgcgccgagt tggctccagc
     3421 acgacggcga ccgccccgag caatacgagg atcggcagtc gtccgctgac ggtcatggtt
     3481 ggcccttcgc gccggtcagc gggggaccgg gaccgaggcg atcgccgtcg cgaggaccgc
     3541 tgcgacgccg acgccttcga gctcggcctc cgggcgcagg gtgagccggt gggcgagggt
     3601 ggcgtgggcg agcgccttga cgtcgtcggg ggtgacgaag gtgcgcccgt tcatccaggc
     3661 ccaggcccgg gacgtggcca gcagcgcggt cgcgccacgc ggggacactc cgagggagag
     3721 cgagggcgac tggcgggtcg cgcggccgat gtcgacgatg tatgccgcga cctccggcgc
     3781 gatccgcacc tgacgcacct cggcggcccc ggcagccagg tcggcggccg acgccaccgc
     3841 ccggatcccc gccgcgacga ggtcacgcgg gtc
//
LOCUS       DAORPW010000224         3835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7587_length_3835_cov_594.378042, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000224 DAORPW010000000
VERSION     DAORPW010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7587_length_3835_cov_594.378042"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1243
                     /locus_tag="PLB86_12280"
     CDS             59..1243
                     /locus_tag="PLB86_12280"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HRC65965.1"
                     /translation="MTTQRPRGFSTTAIHAGQEADESTGAVVTPIYQVSTFKQDGVGG
                     LRGGYEYSRSANPTRAALEECLAALERGSRGFAFASGLAGQDCVVRSLVGPGDHLVVP
                     TDAYGGTYRYVNRVARAHGVEHTVAPMADVAAVQAAIEPGRTKLVWVETPSNPLLGVA
                     DIAALAAVAHEAGALLVVDNTFATSYLQQPLALGADIVTHSTTKYAGGHSDVVGGAVV
                     VAREVGVAAYADAAERIAFHQNAIGAVSGPFDAWLVLRGLKTLAVRMERHCDNAERIV
                     EFLSGHDAVSQVLYPGLAGHAGHEVAARQMRRFGGMVSFRLRRGEDAAVKACAAVSLW
                     TLGESLGGVESLIEHPGRMTHASVVGTALEVPGDLIRLSVGIEDVEDLIDDLREALDI
                     HG"
     gene            1243..2613
                     /locus_tag="PLB86_12285"
     CDS             1243..2613
                     /locus_tag="PLB86_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HRC65966.1"
                     /translation="MGSPTSHQAILVVDVGSTFTKAALVQVSDADGDETGAVLGRAET
                     PTTLDTDVMDGVRSLAAHLGDAAAVDDGRIVACSSAGGGLRLAVIGYERTVTAEAGYR
                     VGLSAGAKVVHVASGPLDTVALAALAEARPDIVLLVGGTDGGNAEVLQHNGIALAAST
                     LPHDVPVVVAGNLDAQPAVAAALQRAGRPHVLGDNVLPEIGVVAPASARVAIREVFLR
                     HVIGGKGLSTDAAFARVVRAATPDAVLAGVEVLAEVLDQDVLVVDVGGATTDVYSAVT
                     PRGDDAGRRTEVVAPLRFSRTVEADLGMRWNAEGVIEAAAREHLPTSTALRRYAARLG
                     EQPAYLPSGPGEAALDLDLARLAAIVAVRRHARPPFPGEAPRALTDLGLVLGSGGVLR
                     HTPSTEARALLEAVAADHAGGWRVPRAPRIGVDAAYLLVVVGLLAPTHPDAARAVARR
                     LALGDG"
     gene            2625..>3835
                     /gene="ilvA"
                     /locus_tag="PLB86_12290"
     CDS             2625..>3835
                     /gene="ilvA"
                     /locus_tag="PLB86_12290"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRC65967.1"
                     /translation="MESLPVTLADVRAARELLAGVVTTTPLEHSRGLSERVGVPVYLK
                     CENLQRAGSFKIRGAYTRMSRLSEAERARGVVAASAGNHAQGVALAAQLLGISATVFM
                     PVGAPLPKLAATRAYGATVQLHGDTLDEALVAALEYAASTGAVLIHPFDHPDIVAGQG
                     TCGLEILEQLPDVKTILVSTGGGGLVGGIAVAAKSLRPDVRVIGVQAEQAAAWPLSLA
                     AGTPVAVDSMQTMADGIAVGRPGPVPFEVVSNLVDDMVTVSEAALSRAVVFLLERAKV
                     VAEPAGAAAVARLLEGPGPLEGPVVAVVSGGNIDPLLLLRIVRHGLTAGGRFLQVTVR
                     VPDRPGSLARLLADLAATTANVVEVNHVRTDSGLAVDEVEIAVDLETRGTAHREEVLR
                     ALRSAGYRLVFG"
BASE COUNT          484 a         1425 c         1365 g          561 t
ORIGIN      
        1 ccgtcgctca gcccgcttgc cacccacgac cggccgcgcg gggctaaagt cgctcgccat
       61 gaccacgcag cgcccccgcg gcttctccac gacggccatc catgccgggc aggaggccga
      121 cgagtccacc ggcgcggtgg tgacgccgat ctaccaggtg tcgaccttca agcaggacgg
      181 cgttggcggg ctgcgcgggg gctatgagta ctcccgctcg gccaacccca cgcgcgccgc
      241 cctcgaggag tgcctcgcgg ccctggaacg gggttcgcgc gggttcgcct tcgcctccgg
      301 gctggccggg caggactgcg tcgtccgctc cctcgtcggt ccgggtgacc acctcgtggt
      361 ccccaccgac gcctacggcg gcacctaccg ctatgtcaac cgggtcgccc gggcgcacgg
      421 cgtcgagcac accgtcgccc ccatggccga tgttgcggcg gtccaggcgg ccatcgagcc
      481 ggggcgcacg aagctcgtct gggtcgagac gccctcgaac ccgctcctcg gcgtcgccga
      541 catcgccgca ctcgccgcgg tggcccacga ggccggcgcc ctgctcgtcg tcgacaacac
      601 cttcgcgacg agttacctcc aacagccgct cgcgctcggt gccgacatcg tcacccacag
      661 cacgacgaag tatgccggtg ggcacagtga cgtcgtcggc ggcgccgtcg tcgtcgcgcg
      721 ggaggtgggt gtcgcggcat acgccgatgc cgccgagcgg atcgccttcc accagaacgc
      781 gatcggagcc gtttccgggc ctttcgacgc gtggctcgtg ctgcgcgggc tcaagaccct
      841 cgccgtgcgg atggagcggc actgcgacaa cgccgagcgc attgtcgagt tcctcagcgg
      901 ccacgacgcc gtgagccagg tgctctatcc cgggctggcc ggacacgccg ggcacgaggt
      961 cgccgcccgg cagatgcggc ggttcggcgg catggtgtcc ttccgactgc gccgcggtga
     1021 ggacgcggcg gtcaaggcct gcgccgccgt gtcgctgtgg acccttggcg agtcgctcgg
     1081 cggcgtcgag tcgctcatcg agcaccccgg ccggatgacc catgcctcgg tcgtcggcac
     1141 cgccctggag gtgcccggtg acctcatccg cctgtcggtc ggcatcgagg acgtcgagga
     1201 cctcatcgac gatctgcgcg aggccctgga catccacggc tgatgggttc accgacgagc
     1261 catcaggcga tcctcgtcgt tgacgtgggg tcgaccttca ccaaggccgc cctcgtccag
     1321 gtgagcgatg ccgacggcga cgagactggc gccgtcctgg gtcgcgccga gaccccgacc
     1381 accctcgaca ccgacgtcat ggacggggtc cgctccctcg ctgcccacct cggtgatgcc
     1441 gccgcggtcg acgacggccg gatcgtggcc tgctcgtcgg caggcggcgg gctgcgcctc
     1501 gcggtcatcg gctatgaacg caccgtgacg gccgaggccg gctatcgcgt gggcctctcg
     1561 gccggcgcga aggtcgtcca cgtggcgtcc ggcccgctcg acacggtggc cctggcggcc
     1621 ctcgcggagg cccgccccga catcgtcctg ctcgttggcg gcaccgatgg cggcaacgca
     1681 gaagtgttgc agcacaatgg gatcgctctc gccgcctcga cgctccccca cgacgtcccc
     1741 gtcgtcgtcg ccggcaacct cgacgctcaa cccgccgtcg cggcggccct ccagcgggcc
     1801 ggtcggcccc atgtgctcgg cgacaacgtg ctgcccgaga tcggggtcgt cgcccccgcg
     1861 agcgcgcgcg tggcgatccg cgaggtgttc ctcaggcacg tcatcggagg caagggcctg
     1921 tccaccgacg ccgccttcgc ccgggtcgtg cgcgcggcca cgcccgacgc ggtcctcgcc
     1981 ggggtcgagg tgctcgccga ggtcctcgac caggacgtgc tcgtcgtcga tgtcggaggc
     2041 gcaaccacgg acgtctattc ggccgtcacc ccacgaggtg acgacgccgg ccgccgcacc
     2101 gaagtcgttg cgccgctgcg gttctcacgc accgtcgagg ccgacctcgg gatgcgctgg
     2161 aacgccgagg gagtcatcga ggcagccgcc cgcgagcacc ttccgacgtc gaccgccctg
     2221 cgcaggtatg ccgcgcgcct gggtgagcag ccggcatacc tcccgtcagg gccgggtgaa
     2281 gcggccctcg acctggacct cgcccggctg gcggccatcg tcgccgtccg acggcacgcc
     2341 cggccgccgt tccccggcga ggcgccccga gcactgaccg acctcggtct ggtgctgggc
     2401 tccgggggcg tgctgcggca taccccgtcg acggaggcac gagccctgct ggaggcggtc
     2461 gcggccgacc atgcgggggg ctggcgggtg ccgcgagcac cacggatcgg ggtcgacgcg
     2521 gcatacctgc tggtcgttgt cggcctgctc gcgccgaccc atccggacgc ggcccgtgcc
     2581 gtagcccgga ggttggccct cggcgacgga tagccttgcc cgccatggag tccctgccgg
     2641 tcaccctcgc tgacgtccga gccgcgcggg agttgctcgc cggagtcgtg acgaccaccc
     2701 ccttggagca cagccgcggg ttatccgagc gggtcggggt gccggtctat ctcaagtgcg
     2761 agaacctcca gcgggccgga tcgttcaaga tcaggggcgc ctacacgcgg atgtcgcggc
     2821 tgtccgaggc ggagcgcgcc cgcggcgtcg tggccgcgag cgccggcaac cacgcgcagg
     2881 gagtggccct ggccgcgcaa ctgttgggca tctccgcgac ggtcttcatg ccggtcggcg
     2941 cccctctgcc caagctcgcg gcgacccgcg cctacggcgc gacggtgcag ttgcacggcg
     3001 acaccctcga cgaggcgctc gtggcggcgc tggagtatgc cgcgtcgacc ggcgccgtcc
     3061 tcatccaccc cttcgaccac ccggacatcg ttgcgggaca agggacctgc gggctggaga
     3121 tcctcgaaca actgcccgac gtcaagacga tcctcgtgtc gaccggagga ggcgggctgg
     3181 tcggcgggat cgccgtggcg gcgaagtcac tgcggcccga cgtgcgagtc atcggcgtgc
     3241 aggccgagca ggctgctgcc tggccgttgt cgttggccgc ggggacgccg gtggccgtcg
     3301 actcgatgca gacgatggcc gacgggatcg cggttgggcg ccccggaccg gtgccgttcg
     3361 aggtggtgtc caacctcgtc gacgacatgg tcaccgtgag cgaggccgcg ctgtcccgcg
     3421 ccgtcgtctt cctcctggag cgcgcgaagg tggtcgccga gccggccgga gcggctgcgg
     3481 tggcccgctt gttggagggt cccgggccgc tggaggggcc cgtcgtcgcc gtcgtgtccg
     3541 gcggcaacat cgacccgctg ctcctgctgc ggatcgtgcg ccacggcctg accgccggcg
     3601 gccgcttcct ccaggtcacc gtgcgggtgc cggaccggcc aggttcgctg gcccgcctgc
     3661 tcgccgacct ggccgcgacc acggcgaacg tcgtcgaggt caaccacgtg cggaccgatt
     3721 ccggcctggc cgtcgacgag gtcgagatcg ccgtcgatct ggagacccgc ggcaccgccc
     3781 accgcgaaga ggtcctgcga gcgctgcgct cggccggcta tcggctggtc ttcgg
//
LOCUS       DAORPW010000225         3823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7627_length_3823_cov_669.012473, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000225 DAORPW010000000
VERSION     DAORPW010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7627_length_3823_cov_669.012473"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="PLB86_12295"
     CDS             complement(<1..1710)
                     /locus_tag="PLB86_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HRC65968.1"
                     /translation="MFWLVAAGVVALIHWMVPHAGWLMAHLVLLGALTHAAMVWSTHF
                     AQALLKTRPGLDERRTQSQRLALLFVGVSLVLVGVAAEIWWSALAGAVLVSVAVIWHG
                     VMLWRRLRASIAPRFRISVYYYLAAAAWVPVGATLGVLLARGPEDEWHGRLLVAHTMV
                     MSLGWIGLTVTGTLVTLWPTMLRARMDPRAERFAQQALPGFAVALIVILVGATAGLRV
                     VTVVGLVGYLLAGAWWGRALVAPLRVRAPGELAPLSVAAALVWAVVAIGWVAGTVALR
                     ADWTEVGDTYGVPTAVLASAFAPQLLVGAMSYLVPAVLGGGPAAVRAASAWVDKWALV
                     RLVVINGGVIVSLLPVPSIVRVLTTALVLTAYGMFVPLLGLAIRASIRARKAAESRGR
                     DVPVPRTELKRRSVWSSGQFVGGVTALAFVVCLGVLADPAAAGLTVAAGEFGAGASAG
                     AAPVAATGHTTTVRVTAMDMRFDPPAVTVPAGDRLVIELVNADPTTSHDLVLANGLGT
                     PRLRPGASTTLDVGVVGGSMDGWCSVVGHRQMGMRFAVTVVGVATPAASAAATTGAAS
                     GHGQ"
     gene            complement(1749..2873)
                     /locus_tag="PLB86_12300"
     CDS             complement(1749..2873)
                     /locus_tag="PLB86_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65969.1"
                     /translation="MPDAAPEAVPDFTARRSVRTALLATAGVSMLAGLYAALLLVVDR
                     EPTAAQRLPDVHGILMVLGFLGTLVALERSVALRQRWAYAAPLALGLGGLALVVPIPL
                     RVGRLLLILGCALAMAVLVHLWRRQEDDPTATQILGAAMALGAAILWTRLDVVDLLPW
                     LVGYIVLTIAAERVELARLTMPPHAGRLLLAIGAAVLMTAALTLLWPSVATRAFGLLT
                     LGLVAWLARSDVARRTIRSTGLPRFSAAALLCGYVWLAVAGVVWVVGGMPRALAAYDT
                     VVHATFLGFAMSMVMAHAPVIFPSVLRRPLPYRSAMWIPLVLLQIGLTVRIGLGDGLG
                     LEGPWQIGAVLNVVALLAFAGTAAWSARSGQRARSAGGRS"
     gene            complement(2866..3267)
                     /locus_tag="PLB86_12305"
     CDS             complement(2866..3267)
                     /locus_tag="PLB86_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRC65970.1"
                     /translation="MAEHIEIVNATTALRLASASHDTAAAPPAAAVLAQMLTTHAHRE
                     EVGLFRVLARQEEFTSEVTTLCGEHGSLEAQLAAIVAGDLDAVNPLIDALRDHINREE
                     NGLFPASAIALSGSEWDEVERTTPPSAVHRA"
     gene            complement(3518..>3823)
                     /locus_tag="PLB86_12310"
     CDS             complement(3518..>3823)
                     /locus_tag="PLB86_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65971.1"
                     /translation="AEREGQTIGVEVKGFPGLGYADPARADQRKRTRPSSQAVHWYSQ
                     AILAAMRLRTKHPEWRSVIALPDHPRYRTLHSETAGSLAAAAIDVWWVDTAGDVSGL"
BASE COUNT          656 a         1333 c         1370 g          464 t
ORIGIN      
        1 ctgcccgtgc cccgacgcgg cccccgtggt cgccgccgcg gacgcagccg gggtggcgac
       61 gccgacgacg gtcacagcga agcgcatccc catctgccgg tgcccgacca ccgagcacca
      121 gccatccatc gacccgccga ccaccccgac gtcaagcgtg gtggaggccc ccgggcgcag
      181 cctcggcgtg ccgagcccgt tggccagcac gaggtcgtgg ctcgtcgtcg ggtcggcgtt
      241 gacgagctcg atgaccagcc gatcgcctgc cggcacggtc acggcagggg ggtcgaaacg
      301 catgtccatc gcagtgactc gcaccgtggt cgtgtgaccg gtcgccgcaa ccggcgctgc
      361 ccccgccgaa gccccggcac cgaactcccc ggccgcgacg gtcagcccag cggccgccgg
      421 gtcggcgagg acgcctaggc agaccacgaa ggcgagcgcc gtgaccccac cgacgaactg
      481 ccccgacgac cacaccgacc gacgcttgag ctcggtgcgc ggaacgggca cgtcgcgccc
      541 acgactctcg gccgccttcc gcgcccggat cgatgcgcga atcgccagcc ccagcaacgg
      601 cacaaacatc ccgtatgccg tgagcaccag tgccgtggtc agcacccgga cgatgctcgg
      661 caccggcaac aggctcacga tgacaccacc gttgatgacg acgagccgca cgagggccca
      721 cttgtccacc caggccgagg cggcgcggac cgccgctgga ccgcccccga ggacggcggg
      781 cacgaggtag gacatcgctc cgaccagtag ttgtggtgcg aacgcgctgg ccaacacggc
      841 cgtcgggacg ccgtaggtgt cgccgacctc agtccaatcg gcgcgcagcg caacggtccc
      901 ggcgacccat ccgatggcga cgacagccca caccagcgct gccgcgaccg agaggggggc
      961 gagctcgccc ggtgcccgca cccgcagcgg ggcaacgagg gcccggcccc accaggcccc
     1021 ggccagcagg tagccgacca gcccgacgac cgtgaccacc cgcagacccg cggtggcgcc
     1081 gaccaggatg acgatgagag caacggcgaa acccgggagc gcctgctgcg cgaagcgttc
     1141 ggctcgaggg tccatgcggg cgcgaagcat cgtcggccac agggtgacga gcgtgccggt
     1201 gaccgtgagg ccgatccagc cgagcgacat gaccatcgtg tgcgcaacga gcaagcgacc
     1261 gtgccattcg tcctcggggc cacgagccag caggacgccc agggtggcgc cgaccggaac
     1321 ccaggcggcc gcagcgaggt agtagtagac gctgatccgg aatcggggcg cgatcgacgc
     1381 gcgcagcctg cgccagagca tgacgccgtg ccagatgacc gcgacgctca ccaggaccgc
     1441 cccggccagc gccgaccacc agatctcggc ggcgactccg acgaggacca ggctcacccc
     1501 gacgaacagc agcgcgagcc gttggctctg ggtccgccgt tcgtccagac ccggccgggt
     1561 cttgagcagc gcctgcgcga agtgggtgct ccacaccatg gcggcatggg tgagcgcgcc
     1621 gagcaggacg aggtgggcca tcaaccaacc ggcgtgagga accatccagt ggatgagcgc
     1681 gacgacaccc gcggcgacca gccagaacaa cgagggatag tctcgccacc cccacgtcgc
     1741 gcgccgcttc atgagcggcc cccggcgctc cgggctcgtt gtcccgatcg cgcgctccac
     1801 gcagcggtcc cggcgaaggc cagcagggcg acgacgttga gcacggcacc gatctgccac
     1861 gggccctcca gcccgagtcc gtctcccagg ccgatccgca cggtaaggcc aatctgcaag
     1921 agcaccaagg gaatccacat agccgagcgg tagggcagcg gccgcctcag caccgaaggg
     1981 aagatcaccg gcgcgtgcgc catcaccatc gacatcgcga agcccaggaa ggtggcatgg
     2041 acgaccgtgt cgtatgccgc gagcgcgcgc ggcatacctc cgacgaccca gaccaccccc
     2101 gcaacggcga gccacacgta gccgcacagc agtgccgccg cggagaagcg cggcaacccc
     2161 gtcgagcgga tcgtgcgccg ggcgacgtcg gagcgcgcca gccaagcgac cagaccgagg
     2221 gtgaggaggc cgaaggcgcg agtggcgacg ctcggccaga ggagcgtcag ggcggcggtc
     2281 atcagcacgg ctgcaccgat ggcgaggagg aggcgccctg cgtgtggcgg catggtgagc
     2341 cgggccaact cgacccgctc ggcggcgatc gtgagcacga tgtagcccac gagccacggc
     2401 agcaggtcga cgacgtcaag ccgggtccac aggatcgcgg cgcccaaagc cattgcggcg
     2461 ccgaggattt gggtggctgt tggatcgtcc tcctggcgcc gccacagatg gacgaggacc
     2521 gccatcgcca gcgcgcagcc gaggatcagc agaagacggc cgaccctgag ggggatcggg
     2581 acgacgagcg cgaggccgcc gaggccgagg gcgagtgggg cggcatacgc ccagcgttgg
     2641 cggagggcga cggaccgttc gagcgcgacg agggtgccga ggaaccccag caccatgagt
     2701 atgccgtgca cgtccggaag ccgttgtgcc gcagtcggtt cccggtcgac gacgaggagc
     2761 aacgcggcat acaggccagc gagcatgctg acccccgcgg tggcgaggag ggccgtgcgc
     2821 acactccgtc gcgcggtgaa gtcgggcact gcctccgggg cggcgtcagg cacggtgcac
     2881 cgccgacggc ggcgtggtcc tctccacttc atcccactcg ctgcccgaga gggcgatcgc
     2941 ggacgcgggg aagaggccgt tctcctcgcg gttgatgtgg tcgcgcaggg cgtcgatgag
     3001 ggggttgacc gcgtccaggt cgccggcgac gatggccgcc aattgggctt cgagagagcc
     3061 gtgttcgccg cagagagtgg tgacctcgga ggtgaactcc tcctgtcgcg cgagcacccg
     3121 gaagaggccg acctcttcac ggtgagcgtg cgtggtgagc atttgggcga ggactgcggc
     3181 agctggcggc gcggccgcgg tgtcgtggct ggcgctcgcc agacggagag cggtcgtggc
     3241 gttgacgatc tcgatgtgct cggccatgaa gtcgccgatc gtggtgatcg actcgcagcc
     3301 gcagtaggag cacatcagcg ggcggccggt tgggcgagca gcgtgtgcgt tgggatccgc
     3361 cgcgcgtgga cggtcatagg agagacttct gattttccac ggttctaagc ctactatttg
     3421 gctcctgccg tccggacact ggccctcgga ccgggattgt gtggctggat gggccaccca
     3481 ggacgtccca gccacacgat cccgagccgt gcgtccgtca gaggccgctg acgtctcctg
     3541 cggtgtccac ccaccagacg tcgatggcgg cggctgcgag cgagccagcg gtttctgagt
     3601 ggagcgtccg gtagcgcggg tggtccggga gggcgatcac actgcgccat tcggggtgct
     3661 tggtccgcag ccgcatcgcg gcgaggatgg cttgcgaata ccaatggacg gcctgactgc
     3721 tcgggcgagt gcgtttgcgc tggtcggccc gggcggggtc ggcgtaaccc agccccggga
     3781 accctttgac ctcgacgccg atcgtctggc cttcgcgctc ggc
//
LOCUS       DAORPW010000226         3802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7699_length_3802_cov_938.799573, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000226 DAORPW010000000
VERSION     DAORPW010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7699_length_3802_cov_938.799573"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1579
                     /locus_tag="PLB86_12315"
     CDS             38..1579
                     /locus_tag="PLB86_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRC65972.1"
                     /translation="MTKSPWIVVKWVAIAAALALLVWIAGKFAAAGSLWAVVGVAFIA
                     MCVLAIYGTTRAVPLKYLFPGLFFLVALQIWPIIFTIATSFTNYGDGHMGTKEESVKY
                     LIAQSVREVEGAPRYAMSVAVPTGADVTTGTITLLLTDPKDGATYAGTPEGLAPLTDG
                     VEKSPTGKVTKANGFTILNAREVNARSEDLSALAVPTEGGGIKTSGLSEAFVGKASMQ
                     YDAAADRMIDTTTGKRYMPQNALWVPEDGQGQSLTSGWQENVGLRNYTEALTNETLRT
                     GFLKILVWNLIFPLVSVISTFLLGMGFALLFNDDRIKAKGAWRSLLILPYALPGFVTA
                     LVWRQMFNQDPNFGLINKTFGWSIDWLGDPTWAKVAILITNLWLGFPYMFLICTGALQ
                     SVPGDVKEAAKIDGASGFRTIRSIVMPLVLVAVGPLLVASYAFNFNNFALIYLLTNGG
                     PFEGNQTQVGSTDLLITYAFRLAIGGTTPNFGFAAAISMFIFIIVALISLQGFRSTAS
                     LEDVN"
     gene            1579..2499
                     /locus_tag="PLB86_12320"
     CDS             1579..2499
                     /locus_tag="PLB86_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRC65973.1"
                     /translation="MTATVSTPSTPPAPSTPTHAFDAPPTAKRRDPWQVVRYLAIGAA
                     LIFALFPIWFIVAAAVNPSGNLNTTQLIPDAFGLGNYTDLTASRPFWSWYKNTLIIAG
                     IGSIAAVFIGVCAAYAFSRLRFQGRRPGLMTLLLIQMFPSLLTFVALFRIFTELGDVI
                     PQFGLNTSLGLILAYLGGAMGGNVWLLKGYFDTVPKELDEAAKVDGASHARVFFTITL
                     PLITPILVTVGMIQFIGLFNEFLLASVFLKDVDAQTLGVGLNALLTADRNKYFGAFCF
                     GAVLASAPIVLIYLSFQRQLIGGLTAGSVK"
     gene            2496..>3802
                     /locus_tag="PLB86_12325"
     CDS             2496..>3802
                     /locus_tag="PLB86_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC65974.1"
                     /translation="MTASFDPALPVTGPVTGLISTPAIGLNRPHHDGSALYVSTETPR
                     LGAVVSLWLRVPAVATAPGEAPFDAAYVRVLRDGEPHFVTAVRDADRSSEREVWWRAE
                     VGCRNPVTSYRWLLSGGAIGWLWVNGAGIHLRDVPDAANFRIIAADEPLPQWPLDAVV
                     YQVFPDRFARSAAADERETPDWAVEAAWSDPVETRSRAGGSRQLYGGDLDGVAEHLDH
                     IAALGADVVYLTPFFPGRSNHRYDAHTFDAVDPLLGGDAALRRLTDAAHARGIRVLGD
                     FTSNHTGVTHEWFEAARADESAPERAFYFFGPGQEYAAWLGVPSLPKLDHSSPELRRR
                     LFEDPRGVIQRWLGPDGGLDGWRVDVANMTGRHRGADLYHQVARDLRAASVAARPDAL
                     VVAEHCHDVSTDLTGDGWHGAMNYAGFTRPVWTWLTSPTNPDAP"
BASE COUNT          546 a         1417 c         1176 g          663 t
ORIGIN      
        1 ccctcgccct cgcccccgca gcagccctgg agtccccatg accaagagcc cctggatagt
       61 cgtcaaatgg gtggccatcg cggccgccct cgccctcctc gtgtggatcg ccggcaagtt
      121 cgccgccgcg ggcagcctct gggccgtcgt cggcgtcgcg ttcatcgcca tgtgtgtgct
      181 cgcgatctac ggcaccaccc gggccgtccc gctgaagtac ctcttcccgg gcctcttctt
      241 cctcgtcgcc ctgcagatct ggccgatcat cttcacgatc gcgacgtcgt tcacgaacta
      301 cggcgacggc cacatgggga ccaaagagga gtcggtcaag tacctcatcg cccagtcggt
      361 cagggaggtc gagggggcgc cgcgctatgc gatgtcggtc gcggtgccga cgggcgcgga
      421 cgtcaccacg ggcaccatca cgctcctgct caccgacccc aaggacggcg cgacgtatgc
      481 cggtacgccg gagggcctcg cgccgctcac cgacggggtc gagaagagcc cgaccggcaa
      541 ggtcaccaag gccaacggct tcacgatcct caacgcccgc gaggtcaacg cccgctccga
      601 ggacctgtcg gcgctcgccg tgccgaccga ggggggcggc atcaagacct cagggctctc
      661 ggaggcgttc gtcggcaagg cgtcgatgca atacgacgcc gccgctgacc ggatgatcga
      721 cacgacgacg ggcaagcggt atatgccgca gaacgcgctc tgggtgccgg aggacggcca
      781 agggcagtcg ttgacgtcgg gctggcagga gaacgtcggg ctgcgcaact acaccgaggc
      841 tttgaccaac gagacgctac gcaccggctt cctcaagatc ctcgtgtgga acctcatctt
      901 cccgctcgtc tcggtgatct cgacgttcct gctgggcatg gggttcgccc tgctgttcaa
      961 tgacgaccgg atcaaggcga agggcgcctg gcgctcgctg ctcatcctcc cttacgcctt
     1021 gccgggcttc gtcacggcgc tggtgtggcg tcagatgttc aaccaggacc cgaacttcgg
     1081 gctgatcaac aagaccttcg ggtggagcat cgactggctg ggtgatccga cctgggccaa
     1141 ggtcgccatc ctcatcacca acctgtggtt gggcttcccc tacatgttcc tcatctgcac
     1201 cggagctctc cagtcggtgc cgggggacgt caaggaggcc gcgaagatcg acggcgcctc
     1261 cggcttccgg acgatccgct cgatcgtcat gccgctcgtg ctcgtcgctg tcgggccgct
     1321 gctcgtcgcg tcctacgcct tcaacttcaa caacttcgcg ctcatctatc tgctgaccaa
     1381 cggcgggccg ttcgagggga accagaccca ggtcggctcg accgacctgc tcatcaccta
     1441 tgcgttccgt ttggccatcg gcggcacgac ccccaacttc ggcttcgcag cggcgatctc
     1501 gatgttcatc ttcatcatcg tcgccctcat ctccctgcag ggtttccgta gcaccgcttc
     1561 tctcgaggac gtgaactgat gaccgccacc gtgtccacgc cgtccacccc gccggcgccg
     1621 tccaccccga cccatgcctt cgacgcccca cctacggcca agcggcgcga cccctggcag
     1681 gtggtgcgct acctggccat cggggcggcg ctgatcttcg cgctcttccc gatttggttc
     1741 atcgtcgccg ccgcggtcaa cccgtccggc aacctcaaca ccacgcagct catccccgac
     1801 gcgttcgggc tcggcaacta caccgatctc acggcctcgc ggccgttctg gtcctggtac
     1861 aagaacaccc tcatcatcgc cgggatcggc tcgatcgccg cggtgttcat cggggtgtgc
     1921 gcggcatacg ccttctcgcg gctgcggttc cagggtcggc gacccggcct gatgaccctg
     1981 ttgctcatcc agatgttccc gtcgctgctc accttcgtcg cgctgttccg gatcttcacc
     2041 gagctcggcg acgtcatccc gcagttcggg ctcaacacct cgctcggcct gatcctcgcc
     2101 tacctcggcg gcgccatggg cggcaacgtg tggctgctca agggctactt cgacacggtc
     2161 ccgaaggagc tcgacgaggc cgccaaggtc gacggggcca gccatgcccg ggtgttcttc
     2221 accatcaccc tgccgctcat caccccgatt ctcgtcaccg tcgggatgat ccaattcatc
     2281 ggcctgttca acgagttcct cctggccagc gtcttcctca aggatgtcga cgcccagacc
     2341 ctcggcgtcg gcctcaacgc gctgctgacc gccgaccgca acaagtactt cggggccttc
     2401 tgcttcgggg cggtcctcgc ctcggcgccg atcgtcctca tctacctctc cttccagcgc
     2461 cagctcatcg gtggtctcac cgccgggtcg gtgaagtgac cgcgtccttc gatccggcgc
     2521 tgccggtcac cgggccggtc accgggctga tcagcacgcc ggccatcggt ctgaaccggc
     2581 cccaccacga cggttcggcg ctctacgtct cgaccgagac gcctcgcctc ggtgcggtcg
     2641 ttagcctgtg gttgcgggtt cccgcggtcg cgacggcccc cggggaggcg cccttcgacg
     2701 cggcatacgt gcgggttctg cgtgatgggg aaccccactt cgtcacggcc gtgcgcgatg
     2761 ccgatcgctc cagcgagcgc gaggtgtggt ggcgcgccga ggtgggttgt cgcaaccccg
     2821 tgacgtccta ccgctggctg ctctccggcg gcgcgatcgg gtggttgtgg gtcaacgggg
     2881 caggcatcca cctgcgcgac gtccccgacg ccgcgaactt ccggatcatc gccgcggacg
     2941 agccgctgcc gcagtggccc cttgacgccg tcgtctacca ggtcttcccc gaccgcttcg
     3001 cccgctcggc cgccgccgac gagcgggaga cccccgactg ggccgtcgag gcggcctggt
     3061 cggacccggt ggagacccgc tcgcgggcgg gtggcagccg ccagctctac ggcggtgacc
     3121 tcgacggcgt cgccgagcac ctcgaccaca tcgcggccct cggcgccgac gtcgtctacc
     3181 tcacgccgtt cttccccggg cgctccaacc accgttatga cgcccacacc ttcgacgccg
     3241 tcgacccgct ccttggcggc gacgcggcac tgcggcggct caccgacgcg gcgcacgcac
     3301 gcggcatacg cgtcctcgga gacttcacca gcaaccacac cggggtcacc cacgagtggt
     3361 tcgaggccgc gcgcgcggac gagtccgccc cggagcgcgc cttctacttc ttcggacccg
     3421 gccaggagta tgccgcgtgg ctcggcgtcc cctcgctccc caagctcgac cacagcagcc
     3481 cggaactgcg tcgccgactg ttcgaggacc cgcgcggggt gatccagcgc tggctcggcc
     3541 ccgatggcgg gctggatggg tggcgggtcg acgtcgccaa catgaccggg cgccatcgcg
     3601 gcgccgacct ctatcaccag gtcgcccgcg acctgcgtgc ggccagcgtt gccgccaggc
     3661 cggatgctct cgtcgtcgcc gagcactgcc acgacgtgtc gacggacctc accggtgacg
     3721 gttggcacgg ggcgatgaac tacgccggtt tcacccgtcc ggtgtggacc tggctcacgt
     3781 cgccgaccaa ccccgacgcc cc
//
LOCUS       DAORPW010000227         3733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_7925_length_3733_cov_809.331974, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000227 DAORPW010000000
VERSION     DAORPW010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7925_length_3733_cov_809.331974"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /gene="recA"
                     /locus_tag="PLB86_12330"
     CDS             <1..819
                     /gene="recA"
                     /locus_tag="PLB86_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRC65975.1"
                     /translation="TVALHAVANAQKAGGIAAFIDAEHALDPEYAKNLGVDIDNLLVS
                     QPDTGEQALEIADMLIRSGAIDVIVIDSVAALVPRAEIEGEMGDSHVGLQARLMSQAL
                     RKLTGALSTTGTTAIFINQLREKIGVMFGSPETTTGGKALKFYASVRLDVRRIETLKD
                     GSEAVGNRTRVKVVKNKVSPPFKQAEFDIIYGQGISREGGLIDMGVDQGFVRKAGAWY
                     TYDGDQLGQGKENARQFLRDNPDLADELEKRIKEKLGVGPRVDKPAVPDVPVDF"
     gene            1083..1526
                     /locus_tag="PLB86_12335"
     CDS             1083..1526
                     /locus_tag="PLB86_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRC65976.1"
                     /translation="MLALRPHSRVELERKLARRGCDPQVAAEVLDRLTEVGLVDDAAY
                     AHLLVQSRSRTNGLSGVALRRELREKGVDEDLAKDAVGALDDETERARAEELVAKKLR
                     TMGGLDPQVQARRLAAMLARKGYPAGVAYAVVRDAVNGAVEHHHD"
     gene            1612..2043
                     /locus_tag="PLB86_12340"
     CDS             1612..2043
                     /locus_tag="PLB86_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC65977.1"
                     /translation="MDYRDYDTRLAAYAVIRDDAGRVLLALWNEADLPKWTMPGGGVE
                     LDETVQEAVVREVHEETGYDVVVGQLLGVHTFVLPPHVRVNTTDRPLKSVRVVFEARI
                     VGGELRHEIGGTTDEARWFDPGAISDLPRVSLVDIALGMAH"
     gene            complement(2104..2532)
                     /locus_tag="PLB86_12345"
     CDS             complement(2104..2532)
                     /locus_tag="PLB86_12345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HRC65978.1"
                     /translation="MTATTLLDFGDEPYVLLTTFRANGDRVGTPVWVARDGDHLVVMT
                     AAGTGKVKRIERNALVELTPCDRRGRPKPWAVTLTGRAEVRESEGDADATRALVKAKY
                     GVPYRIFTGIERIVRRLKQSPRVALWIHPTADVPDFLATP"
     gene            3163..>3733
                     /locus_tag="PLB86_12350"
     CDS             3163..>3733
                     /locus_tag="PLB86_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147790.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRC65979.1"
                     /translation="MSTTTAAAPKTYDVRTHGCQMNVHDSERLAGLLETAGYVDLASL
                     PADARPETADVVVFNTCAVRENASNKLYGNLGQLRPAKLANPDLQIAVGGCLAQNDRE
                     SIVARAPWVDVVFGTHNIGSLPALLDRARHNKRAEVEILESLSTFPSTLPTRRDSAYA
                     AWVSISVGCNNTCTFCIVPSLRGKEQDRRA"
BASE COUNT          612 a         1295 c         1263 g          563 t
ORIGIN      
        1 acggtcgctc tgcacgccgt ggcgaacgcc cagaaggcgg gcggcatcgc ggccttcatc
       61 gacgccgagc acgccctcga cccggagtac gccaagaacc tcggcgtcga catcgacaac
      121 ctgctggtca gccagcccga cacgggggag caggcgctgg agatcgccga catgctcatc
      181 cgctccggcg cgatcgatgt catcgtcatc gactccgttg cggcgctcgt gccccgcgcc
      241 gagatcgagg gcgagatggg cgacagccac gtcggcctcc aggcccgcct catgtcgcag
      301 gcgttgcgca agttgactgg cgccctgagc accaccggga ccacggcgat cttcatcaac
      361 cagttgcgcg agaagatcgg tgtgatgttc ggaagtcccg aaaccactac tggcggtaag
      421 gcgttgaagt tctacgcctc cgtgcggctg gacgtccgac ggatcgagac gctcaaggac
      481 ggctcagagg cggtcggcaa ccgcacccga gtcaaggtcg tcaagaacaa ggtgagcccg
      541 ccgttcaagc aggccgagtt cgacatcatc tacggccagg gcatctcccg cgagggaggc
      601 ctgatcgaca tgggcgtcga ccagggcttc gtgcgcaagg ccggcgcttg gtacacctac
      661 gacggcgacc agctcggcca gggcaaggag aacgcccgcc agttcctccg cgacaacccc
      721 gacctggccg acgagctcga gaagcggatc aaggagaagc tcggcgtcgg gccgcgcgtc
      781 gacaagccgg ccgtgcccga cgttccggtc gacttctgag catgacgcct gacggtatgc
      841 cgcgcccgga ctccccgggc gcggcacgcc aggctgcggc gttggcagcc ctacgagccg
      901 ccgaggcggc cgctcggcag ggccctgagc tatcggcccc ggcaggtcca ccggacccaa
      961 gtgctcgtga ctcacatgcc ccgagtgctg gtgcgcccca ggccccgacg gtcgccaatc
     1021 cgcggatcgc tgacgactcc gagcccgatc cccggtctgt tgcgcacggc atcgcgttgc
     1081 gggtgctggc gctgcggccg catagtcggg tcgagctgga gcgcaagctg gcccggcgcg
     1141 gctgcgaccc gcaagtggcg gccgaggtgc tcgaccggct caccgaggtg gggctcgtcg
     1201 acgacgcggc atacgcccac ctgctggtgc agtcgcgaag ccgcaccaac ggtctgtccg
     1261 gtgtggcgct gcgccgggag ctacgcgaga agggggtcga cgaggacttg gcgaaggacg
     1321 cggtcggtgc cctcgacgac gagaccgagc gggcgcgcgc ggaggagctc gtcgccaaga
     1381 agctgcggac catgggcggc ctcgacccgc aggtgcaggc ccgccggttg gccgccatgc
     1441 tcgcccgcaa gggttacccg gcaggcgtcg cgtatgccgt cgtccgtgac gcggtcaacg
     1501 gggcagtcga gcaccaccac gactgaggtt ccgccgcgca ctgggggttg ggcctgtgtc
     1561 gaggcgccac ccggactgag ctcggtgcgc gtgcagatac cgtgaatcct cgtggactac
     1621 cgcgactacg acacccggct cgccgcctat gccgtcatcc gcgatgacgc cgggcgggtg
     1681 ctgctggcct tgtggaacga ggcagacctg cccaagtgga cgatgcccgg gggcggcgtc
     1741 gagttggacg agaccgtgca ggaggcggtc gtccgcgagg tgcacgagga gaccggctat
     1801 gacgttgtgg ttggccagtt gcttggagtc cacaccttcg tcctgccccc gcacgtgcgg
     1861 gtcaacacca ccgatcgacc gctgaagtcg gtgcgggtcg tgttcgaggc gcggatcgtg
     1921 ggtggggagt tgcgccacga gattggcggc accaccgacg aggcgcgctg gttcgaccca
     1981 ggagcgatca gcgacctgcc gcgggtcagc ctcgtcgaca tcgccctcgg catggcccac
     2041 tgacaagcca ctccgagggg cgtcgctgag gagccattgc ggcccgtcgg gccgtcggtt
     2101 aggtcagggt gtggccagga agtcggggac gtcggccgtg gggtggatcc acagggccac
     2161 tcggggcgac tgcttgagtc gccggacgat ccgttcgatc cccgtgaaga tccgatatgg
     2221 cacgccatat ttcgctttca ccagggctcg ggtggcgtcg gcgtcaccct cagattcgcg
     2281 gacctcggcc cgcccggtca gcgtcaccgc ccagggcttg ggccgtccgc gccggtcgca
     2341 gggggtcagt tccaccaggg cgttgcgctc gatccgcttg accttgccgg tgccggccgc
     2401 tgtcatgacg accaggtgat caccgtcgcg cgcgacccag acgggagtgc cgacccggtc
     2461 gccgttggcg cggaaggtgg tcagcaacac gtagggctca tcaccaaagt ccaggagagt
     2521 ggtggctgtc atgatctgca ccctaggcga caccacgaca gccactcacc gcgccgcatc
     2581 gccaacccgc gcctctggca cagtgatagc gcttgcacgg tcatgtttgc gggtgtgtcg
     2641 ggctgtcaga cccggagata gcgctcgcat ggtcatgttt gcgggtgttt ttggctgtca
     2701 gacccggaga tagcgctcgc atggtcatct ttggggtggg tcaccacgtg agcgagcacc
     2761 acggccgatc agctaatata ccctccgggg tatacgccct ccggggcgga aaccccgacc
     2821 cacgactttc gacgacgagg agaacgacgt atgtgctctc gggtgacgtg ccgccgctgc
     2881 ggcaaggcga cctggtccgg ctgcgggcag cacgttcagc aggtcatggc aggcgtcccg
     2941 aaggcgcagc agtgcgcctg ccccccgccc gagccgggtg gcctgttcaa gaggctcttc
     3001 ggtcgcggct gatcctcagc caacccaggg ctggttgcct gctcaccgct gccgatcagg
     3061 cggccccaca cgcacgaggt atgccgtccg cgcaccgtga gcgggcggca tacctttgcg
     3121 cggcgttgcg ccccacaaac acgccttacc cttgacggtg ccatgagcac cacgactgca
     3181 gctgccccca agacctacga cgtccgcacc cacgggtgcc agatgaacgt ccacgactcc
     3241 gagcgccttg ccggcctgct cgagacggcg ggctatgtcg acctggccag cctccccgcc
     3301 gacgcccgcc ccgagaccgc cgacgtcgtc gtcttcaaca cctgcgcggt ccgcgaaaac
     3361 gcgagcaaca agctctacgg caacctcggc caactgcgcc cagccaagct ggccaacccc
     3421 gatctgcaga tcgccgtcgg tggctgcctg gcccagaacg accgcgaaag catcgttgcc
     3481 cgcgccccct gggtcgatgt cgtcttcggc acccacaaca tcggcagcct ccccgcgctg
     3541 ctggatcgag cccgccataa caagcgcgcc gaggtcgaga tcctcgagag cctctcgacc
     3601 ttcccctcga cgctgcccac ccgccgcgac agcgcgtatg ccgcgtgggt ctcgatcagc
     3661 gtgggctgca acaacacgtg caccttctgc atcgtcccga gcctgcgcgg caaggagcag
     3721 gaccgccggg ccg
//
LOCUS       DAORPW010000228         3652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_8235_length_3652_cov_907.958577, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000228 DAORPW010000000
VERSION     DAORPW010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8235_length_3652_cov_907.958577"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /gene="prfA"
                     /locus_tag="PLB86_12355"
     CDS             complement(<1..1030)
                     /gene="prfA"
                     /locus_tag="PLB86_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRC65980.1"
                     /translation="MSDHTTEAAHALLASAAPLVAEHAELERSMADPELHADQSALRR
                     TTKRYAALGPVVAAYRALAAARADLAAAHELAVEDPDFAAELPAIEAVSALAQGRLHT
                     LLLPRDPDDDRDAILEVKAGEGGDESALFAGDLLRMYLRYAERRGWKTEVLDETQSDL
                     GGVKEARIALRAKGTPDIGEAPWARLKFEGGVHRVQRVPVTESQGRIHTSAAGVLVMP
                     DIDEGDDEVEIAPADLRIDVYRSSGPGGQSVNTTDSAVRITHLPTGVVVSCQNEKSQL
                     QNKESALRVLKARLRQAAREAAEATASAARRSQVRTVDRSERIRTYNFPENRIADHRT
                     GFKAYNLDV"
     gene            complement(1161..1379)
                     /gene="rpmE"
                     /locus_tag="PLB86_12360"
     CDS             complement(1161..1379)
                     /gene="rpmE"
                     /locus_tag="PLB86_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRC65981.1"
                     /translation="MKKDIHPAYGETQVTCTCGNTFVTRSTAKNGVIHADVCAACHPF
                     YTGKQKILDTGGRVARFEARYGKKAATK"
     gene            1597..2241
                     /locus_tag="PLB86_12365"
     CDS             1597..2241
                     /locus_tag="PLB86_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRC65982.1"
                     /translation="MTRWIDVAHDVFVLAGVLAFAMSGALQAVGRRMDVVGICSLGVV
                     TAVGGGILRDLLIGAVPPAALTDWTLVPVALVGSLIVFWSHGRWTVPRRPMLVLDALG
                     LGLFCVEGTVKALGFGLNPYASAVVGMLTGVGGGVIRDVLAGQIPSVFRHGSRLYVIP
                     ALAGGGITAALWSAGHFSLLALLAVAATVSAIRIASLRFRWRAASPRDLDLDVG"
     gene            2352..3500
                     /locus_tag="PLB86_12370"
     CDS             2352..3500
                     /locus_tag="PLB86_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRC65983.1"
                     /translation="MTPDEARDIARLGGRAATGIAGLVEHTHHGITERVYRTVGRLTG
                     GASRPIHVTHDLVARHTFFWVQRGLEAGAWAAQVIAARAMGGERSSRSGLDGVRAQLA
                     LGIVNGVTGDRLAADASTLAIPMSLRHEGRDVAPTAEGLRAAYGMGHERIVVFVHGLV
                     ETEHAWRFRSRQRWGEAGVSYASQLAADTDWVPLMVRYNSGAPIPANADAFADLLGEV
                     VRCWPGPLREVVIVGHSMGGLVALRALAHGDAAWTRAVRAVITVGSPREGAPLERFAV
                     VAEEVAESSGHGRWFGGLVGMRSDGIRDLRHPIDHPPLPDWVQEYAVLATLTPDSWHP
                     AVPRVGDGLVPVPADPSEHTVVVTGLHHLDLLNHPSVYAQLRAWLDPE"
BASE COUNT          457 a         1251 c         1344 g          600 t
ORIGIN      
        1 cgacgtcgag gttgtaggcc ttgaacccgg tgcggtgatc ggcgatccgg ttctcgggga
       61 agttgtaggt gcggatccgc tcggaacggt cgacggtgcg gacctggctg cggcgcgccg
      121 cggacgccgt cgcctcggcg gcctcgcggg cggcctgacg caggcgggcc ttgagcaccc
      181 gcagcgccga ctccttgttc tgcagctggg acttctcgtt ctggcacgac acgaccacac
      241 cggtcggcag gtgggtgatc cgcaccgccg agtcggtcgt gttgaccgac tgcccgccgg
      301 ggccgctgga gcggtagacg tcgatccgca ggtcggccgg cgcgatctcg acctcgtcgt
      361 cgccctcgtc gatgtcgggc atgacgagca ccccggcagc cgaggtgtgg atgcgcccct
      421 ggctctcggt gacgggcacc cgctggaccc gatgcacgcc gccctcgaac ttcaaccgcg
      481 cccacggcgc ttcgccgatg tcgggagttc ccttggcgcg caatgcgatt cgtgcctctt
      541 tgacgccgcc gaggtcggac tgcgtctcgt cgagcacctc ggtcttccaa ccgcgacgct
      601 cggcgtagcg caggtacatc cgcagcaggt caccggcgaa cagggccgac tcatccccgc
      661 cctcccccgc cttgacctcg aggatcgcgt cccggtcgtc gtcgggatcg cgcggcagca
      721 ggagggtgtg caggcggccc tgggccagcg ccgagaccgc ctcgatcgcc ggcagttccg
      781 ccgcgaagtc cgggtcctca acggcgagtt cgtgagccgc cgccagatcc gcgcgcgccg
      841 ccgcgagcgc ccggtatgcc gcgacgaccg ggccgagcgc ggcatacctc ttggtcgtgc
      901 ggcgcagcgc gctctggtcg gcgtgcaact ccgggtcggc catcgagcgt tccagctcgg
      961 cgtgctcggc gacgagcggc gccgccgagg cgagcagtgc gtgcgccgcc tcagtggtgt
     1021 gatcgctcat cgcaggctcc gatccggtca gtcagtggct caggcggcac gcggacacac
     1081 cgccgcagga acacgaacgc cggccccgcg gcataccctc cgcgtggtgc gggggatgcg
     1141 ccgggccggc ggacgggacg ctacttggtg gcagccttct tgccgtaacg cgcctcgaag
     1201 cgcgccacgc ggccaccggt gtcgaggatc ttctgcttgc ccgtgtagaa cgggtggcag
     1261 gccgcgcaca cgtcggcgtg gatgacgccg ttcttggcgg tgctgcgggt gacgaacgtg
     1321 ttgccgcagg tgcaggtcac ctgggtctcc ccgtaggcgg ggtggatgtc cttcttcacg
     1381 gttctctcct tgtcggtgat ggcgccgggt cggctgggtc gtgccttggt atgccgtgaa
     1441 ccgggccgag ggtcaagtgt gccagacccc agacgggtga cctaacgcgg cgttgcgctg
     1501 cggcattccc gcagcctggc gagcgacgcg tgagggaccc gtgagggacc gctgagggag
     1561 gcgtgaggaa cccgcgaggg actaccgtgg ggcgccgtga cccgatggat cgacgtggcc
     1621 cacgacgtgt tcgtgctcgc cggcgtcctg gccttcgcga tgtccggcgc gctgcaggcg
     1681 gtcggtcggc ggatggacgt cgtcggcatc tgctcgctcg gcgtggtcac cgccgtcggg
     1741 ggagggatcc tgcgcgacct gctcatcggc gcggtgcctc cggccgccct caccgactgg
     1801 accctggttc cggtggcgtt agtcggctcc ctcatcgtct tctggtcaca cggtcggtgg
     1861 acggtgccga ggcggccgat gctcgtcctg gatgccctcg ggctggggct gttctgtgtc
     1921 gaggggaccg tcaaggcgct gggtttcggg ctcaatccct atgcctccgc cgtggtcggc
     1981 atgctcaccg gggtcggcgg tggcgtcatc cgggacgtgt tggccggaca gatcccgtcg
     2041 gtgttccgcc acgggtcccg gctctatgtc atcccggcct tggccggcgg tgggatcacg
     2101 gccgcgctgt ggagtgcggg gcacttctcg ttgctcgccc tgctggccgt cgctgcgacc
     2161 gtgtcggcca tccggatcgc ctcgctgcgc ttccggtggc gggctgccag cccccgggac
     2221 ctcgacctcg acgtggggtg acatccgtgg ggttgacata cgtggggtga ccgtcggggg
     2281 tgccacaggt caccccctaa gttacgcgac cgtaggtaca gtgaagagat gctgacgtca
     2341 gcatcccggt catgaccccc gacgaggcgc gcgacatcgc ccggcttggg ggacgcgctg
     2401 ccacgggcat cgccggcctc gtcgagcaca cccaccacgg catcaccgaa cgggtctacc
     2461 gcacggtggg ccgcctgacc ggtggggcga gccggccgat ccacgtgacc cacgacctgg
     2521 tggcacggca taccttcttc tgggtgcagc gggggctgga agcgggcgcc tgggcagcgc
     2581 aggtcatcgc ggctcgcgcc atgggcgggg aacgcagcag ccgcagcggc ttggatggcg
     2641 tgagggcgca gctggccctg ggcatcgtca acggggtcac cggcgaccga ctggccgcag
     2701 acgcctcgac gttggcgatc cccatgagcc tgcgacacga gggccgcgat gtcgcgccga
     2761 ctgcggaggg gctgcgcgcg gcatacggga tgggccatga gcggatcgtc gtcttcgtcc
     2821 acggcctggt cgagaccgag cacgcctggc gattccgctc gcggcagcgg tggggcgagg
     2881 cgggcgtgag ctacgcctcg caactcgcgg cggacaccga ctgggtgccg ctcatggtgc
     2941 gctacaactc tggtgccccg atcccggcga atgccgacgc cttcgccgat ctgctgggcg
     3001 aggtggttcg gtgctggcca gggccgctgc gcgaggtggt gatcgtcggg cattcgatgg
     3061 gtggccttgt ggcgttgcgg gccctcgcgc acggcgatgc cgcctggacc cgcgcggtgc
     3121 gagccgtcat caccgtgggc agccctcgcg agggcgcgcc actggaacgc ttcgcggtcg
     3181 tcgcggagga ggtcgccgag agcagcggcc acggacggtg gttcggcggg ttggttggga
     3241 tgcgcagcga cgggatccgt gacctccgcc acccgatcga ccacccgccc ctgccggact
     3301 gggtgcagga gtatgccgtc ctcgccacgc tcacgcccga ctcctggcac ccggccgtcc
     3361 cgcgggttgg tgacggtctg gtgccggtgc cagccgaccc gtccgagcac accgtggtcg
     3421 tgaccggcct gcaccacctg gacctgctca accacccgag tgtctatgcc cagttgcgag
     3481 cctggctcga tcctgagtga ggggccggtg gggcgggcca cttcagcggg tgcgcttcgg
     3541 gccgaaactc gggcaactcg acactcgcgg ggttgccacg cctcacccag gctgactcgg
     3601 tcgggcaact cgacactcgc ggcctttcac cggtcggcca cctggcccca gc
//
LOCUS       DAORPW010000229         3634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_8302_length_3634_cov_892.595418, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000229 DAORPW010000000
VERSION     DAORPW010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8302_length_3634_cov_892.595418"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1427)
                     /locus_tag="PLB86_12375"
     CDS             complement(3..1427)
                     /locus_tag="PLB86_12375"
                     /inference="COORDINATES: protein motif:HMM:NF005858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRC65984.1"
                     /translation="MTEPLLRGLLDPTRADALVRVADTTLSYAALLTSATAVAEAMAR
                     ESLSRIAIDATPTMDTVISVVGAILAGVEVVPIPADAGPGERAHILNDSGADLWSATA
                     PNGGAADGMAERAAESGRAAYRLPEVSPDAGAFVLYTSGTTGLPKGVRLSRRAVAAGL
                     DALADAWAWTADDVLAHGLPLFHVHGLVLGVLGPLRIGGGLRHVGRPTPSAYAAAAHA
                     GATMFFAVPTIWSRVVAEPESARALRSARLLVSGSAALPVPVFEGLRELTGHEVCERY
                     GMTETLITVATRADGVRRAGCVGVPVAGVKTRVVDDDGAPVPADGTSLGELQVCGPTL
                     FDGYLGVPEATAAAFTADGWFRTGDVATIAADGTHRIVGRASVDLIKSGGYRIGAGEI
                     ESVLLGHPAVAECAVIGVPDDDLGQRIVAVVVPAGPAPDELATELIDLVVAQLSVHKR
                     PREVRFVAALPRNEMGKVQKTRLT"
     gene            complement(1424..2032)
                     /gene="thpR"
                     /locus_tag="PLB86_12380"
     CDS             complement(1424..2032)
                     /gene="thpR"
                     /locus_tag="PLB86_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRC65985.1"
                     /translation="MAHTITGWQAGSMGQRMFLAVVPPEPVREDLEAFLDVRPGMRWT
                     HPLHWHVTLAFFASVPEHRIDELIERLATKASKRHTFTTGLAGAGAFPNPARANVLWL
                     GLDCGTDELTHLAASGRAAGAKVGAPPDAQKFRPHLTLARPRPPIEATRWLRVLDSYR
                     SPVWEVVDIALIASYLGEGSGGGSRYETVAQFALGRPGAGTP"
     gene            2031..2540
                     /locus_tag="PLB86_12385"
     CDS             2031..2540
                     /locus_tag="PLB86_12385"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRC65986.1"
                     /translation="MGFPENVLAQGEKVERSLHPHPLTVFGPTLLGVVLIALAVVTSW
                     VTPDDATGNRLQWGAISVLVVLALALVVVPYLRWRTTHYVITSHRVMVRKGILSKSGK
                     DITLSKITDVSFRQTFFDRIIRSGTLSIESAGDSADELLRNIPRSDEIQQLINRLIDE
                     DAKRRSGHS"
     gene            2712..>3634
                     /locus_tag="PLB86_12390"
     CDS             2712..>3634
                     /locus_tag="PLB86_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRC65987.1"
                     /translation="MEDLPLWLRLAIILLLVAGNAFFVGSEIAISSARRSRIRQLAEE
                     GDSRAKTVKLLHEEPERFYSVTQIGITLVSLGLGAVGMETLHTVVDPWVVAIFTALGD
                     HDALHAAAHTVSYVIGFTVVSFIHIVGGELAPKVLAFHRAEPLSLRVGRLINVMYLTF
                     RPLIAVMKWASDLLLRLSGQGNLGGHGESHFTMSVEEIRMILEASEKDGMLDPEETEM
                     IRGVFELDEQTVRDAMVPRTQIRALEQDATLGDALRYFRDIPHARFPVYVDSLDRITG
                     VVAIKELLTYVAEHSGSDSFSKASKKPVKDFA"
BASE COUNT          561 a         1306 c         1218 g          549 t
ORIGIN      
        1 cctcaggtga gccgggtctt ctggaccttg cccatctcgt tgcgggggag ggcggcgacg
       61 aaacggacct cacgcgggcg cttgtgcacc gacaactgtg cgacgacgag gtcgatgagt
      121 tcggtcgcca actcatcggg cgccgggcct gcgggcacga cgaccgcgac gatccgctgc
      181 cccaggtcgt cgtccggcac cccgatcacg gcgcactcgg cgacggcggg atgaccgagg
      241 aggacgctct cgatctcgcc cgctccgatg cggtaaccgc cggacttgat gaggtcgacc
      301 gacgcccgcc cgacgatccg gtgggtgcca tcggcggcga tcgtcgcgac gtcgccggtg
      361 cggaaccacc cgtccgcggt gaacgccgca gccgtcgcct ccggcacccc gaggtagccg
      421 tcgaacagcg ttgggccaca gacctgtagc tcgccgaggc tcgtcccgtc ggccggcacc
      481 ggcgccccgt cgtcgtcgac cacgcgggtc ttcaccccgg ccaccggcac cccgacacac
      541 ccggctcgcc gcaccccgtc ggcccgggtc gccacggtga tcagcgtctc ggtcatgccg
      601 tagcgctcgc acacctcgtg accggtcaac tcccgcaagc cctcgaacac cgggaccggc
      661 agcgccgccg agcccgacac gagcagccga gccgagcgca gtgcgcgggc gctctccggc
      721 tcggcgacga cccgagacca gatcgtcggc acggcgaaga acatcgtcgc gcctgcgtgg
      781 gcggcggcgg catacgcgct gggggtcggt cggcccacgt gccgcaaccc gcccccgatc
      841 cgcagtgggc cgagcacccc cagcacgagc ccgtggacgt ggaacaacgg aagcccgtgc
      901 gcgagaacgt catcggcggt ccaggcccac gcgtcggcga gcgcgtcgag ccccgcggcc
      961 accgctcgac gagacagtcg cacgcccttg ggcagccccg tcgtccctga cgtgtagagg
     1021 acgaacgcgc ctgcgtcggg ggagacttcc ggcagccggt atgccgccct cccgctctcc
     1081 gccgcgcgct cggccatacc atccgccgcg cccccattcg gagccgtcgc ggaccagagg
     1141 tcggcccccg agtcgttcag gatgtgggct cgctcgccgg gcccggcatc ggccggaatg
     1201 ggaacgacct cgaccccggc caagatggca ccgaccacgc tgatgaccgt gtccatcgtc
     1261 ggtgtggcgt caatagctat gcgggacaac gactcccgag ccatcgcctc ggcgacggcc
     1321 gtggcggagg tgagcagcgc ggcatacgag agggtcgtgt ccgccacccg cacgagagcg
     1381 tcggcgcggg tcgggtcgag gagcccgcga aggagcggtt cggtcatggc gtgcccgcgc
     1441 ccggccgacc gagcgcgaac tgcgcgacgg tctcgtagcg ggagccgcca cccgacccct
     1501 cgccgaggta ggacgcgatg agggcgatgt cgaccacctc ccacaccggg ctccggtagg
     1561 agtcgaggac ccgcagccag cgggtggcct cgatgggcgg gcgcggtcgg gccagggtga
     1621 gatgcggcct gaacttctgc gcgtccggcg gagctccgac cttggcgccc gcggcgcgtc
     1681 ccgaagccgc caggtgcgtc aattcgtcgg tgccacagtc taatccgagc cacaggacgt
     1741 tggcgcgggc cgggttgggg aaggcgcccg ctcctgcgag tccggtcgtg aaggtatgcc
     1801 gcttgctcgc cttcgtcgcc agccgctcga tgagctcgtc gatccggtgc tcaggcaccg
     1861 acgcgaagaa ggcgagggtg acgtgccagt gcagggggtg cgtccacctc atcccgggcc
     1921 ggacgtcgag gaaggcctcg agatcctcac gaaccggctc gggaggcacg acggcgagga
     1981 acatccgttg gcccatgctg ccagcctgcc accccgttat cgtgtgggcc atggggtttc
     2041 ccgagaacgt gctcgcccaa ggcgagaagg tcgaacgcag cctgcacccg cacccgttga
     2101 cggtgttcgg gccgaccctg ctcggggtgg tcctcatcgc cctggctgtc gtcacgtcgt
     2161 gggtgacgcc cgacgatgcg accggcaacc gtctgcagtg gggcgccatc tccgtcctgg
     2221 tcgtgctcgc cctcgcgttg gtcgtcgtgc cgtatctgcg ctggcgcacc acgcactacg
     2281 tcatcaccag ccaccgggtc atggtgcgca agggcatcct cagcaagtcg ggcaaggaca
     2341 tcaccttgtc gaagatcacc gatgtgtcct tccgccagac cttcttcgac cggatcatcc
     2401 ggtcaggcac cttgagcatc gagtcggccg gcgactcggc cgacgaactc ctgcgcaaca
     2461 tcccccgcag cgatgagatc cagcagctca tcaaccggct catcgacgag gacgccaagc
     2521 gccgctccgg ccactcctga cggccgaccg ccggtggcgg cggcgttccg caccggtggc
     2581 ggcgtcggcg cgtccgctcg cggattcggg acgttcatcc cccggtgtag cgccgttcgc
     2641 ttctcgtaca ctcgcccacg ggtgcaggtg tcggtccgcg accctgcgaa gaccccgagg
     2701 agtggtcttt catggaggac ctgccgttat ggctgcggct ggcgatcatc ctcttgctcg
     2761 ttgccgggaa cgccttcttc gtgggttcgg agatcgccat ctcctccgcc cgccgaagtc
     2821 gcatccggca gctcgcggag gaaggtgaca gccgggccaa gaccgtcaag ttgctccacg
     2881 aggagcccga acggttctac tcggtcaccc agatcggcat caccctcgtc tcgctcgggt
     2941 tgggcgccgt cggcatggag acgctgcata cggtcgtcga cccctgggtg gtggcgatct
     3001 tcaccgcttt gggcgaccac gacgcgctgc acgccgctgc ccacacggtc agctacgtca
     3061 tcgggttcac cgtggtgtcg ttcatccaca tcgtcggcgg tgagttggcc ccgaaggtcc
     3121 tcgccttcca ccgcgccgag ccgctgagcc tgcgggtcgg gcgcctcatc aacgtcatgt
     3181 acctcacctt ccgtccgctc atcgcggtga tgaagtgggc ctccgacctg ttgctgcgcc
     3241 tttcgggtca gggcaacctc gggggccatg gcgagagcca cttcacgatg agcgtcgagg
     3301 agatccggat gatcctcgag gcgagcgaga aggacgggat gctcgatccc gaggagaccg
     3361 agatgatccg cggcgtcttc gagttggacg agcagacggt ccgcgacgcg atggtgccgc
     3421 gcacccagat ccgcgccctc gaacaagacg ccaccctcgg cgacgccctg cgctacttcc
     3481 gggacatccc gcacgcccgg ttcccggtct atgtcgacag cctcgaccgg atcaccggcg
     3541 tcgtcgccat caaggaactg ctcacctacg ttgccgagca cagcggttca gacagcttca
     3601 gcaaggcgag caagaagccg gtcaaggact tcgc
//
LOCUS       DAORPW010000230         3609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_8411_length_3609_cov_610.576252, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000230 DAORPW010000000
VERSION     DAORPW010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8411_length_3609_cov_610.576252"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLB86_12395"
     CDS             <1..234
                     /locus_tag="PLB86_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC65988.1"
                     /translation="QFLVEASLLGLVGGLAGIALGLLGAAILPNVVSFPVAISPVAAA
                     AGLGVSLVIGITAGVYPASRAARLAPIEALRSE"
     gene            434..1225
                     /locus_tag="PLB86_12400"
     CDS             434..1225
                     /locus_tag="PLB86_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65989.1"
                     /translation="MSGLIAAVDGTTMQVQSSTKQTAVGWTATTSVARTAAGTLADIV
                     AGSCVVVRSASSDGSAPDAGGGVTATSVQVSAAVNGACADVFGGGIRGGGGIPSGMPT
                     GQPGVDQLPPGATDGAPGGPGGRVPGGFGVVGLVTQVGSGTFTVASLTRAGDGGSGTA
                     SAAPAQTTPVTVTTSASTTVTTTKVAAAGDIAVGLCATAIGPTDDIGKVTATSIVLRD
                     AVNGACTSTGGFDGPGAPGAGFPGGGGPGGGGPGGVAPTSAATNG"
     gene            1218..>1850
                     /locus_tag="PLB86_12405"
     CDS             1218..>1850
                     /locus_tag="PLB86_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRC65990.1"
                     /translation="MAERVLRIKRRRWLPMVGVGGAILLVAVGGTYVANGVSAGSETA
                     ALRTGQVTTGSIEQTLTLTGSVTRVSQMTAHFPVAGTVTGVSVAVGDVVTAGQALATL
                     DTGPLNQAVLDAKSTLDQAKATLANDTTTASSSSSSSSASSSGTSSSSPTSSSSRTSA
                     SSSPSTSAGGGGGGGGQNQTTSALAAVAAAQALAKTACAPVLPVATATPTS"
     assembly_gap    1851..1950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1951..3042
                     /locus_tag="PLB86_12410"
     CDS             <1951..3042
                     /locus_tag="PLB86_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65991.1"
                     /translation="PTSTATSTATSTQTATSTVTSTTTTSPPTAQEIADCLGALSAVT
                     AAQQAAGVALTSYAGSLSAAAAQAAAQAAAQAAAQAAAQAQAAAQAKAAAAAAQQAAA
                     AVRSSSGTVSEAKLITDRANVTNAEAALVKAQTNLAAATLTAPMAGRVGAIAWAVGDT
                     ATTANGIQIVGSGAAKVTIQVPAANLAAIAVGQAARVTVTGIPVMDGTVSQVALLPTS
                     TTGSSASTYAVDVLVPNAPDAVGTGGKASVAVVTTKVDGVLTVPASAVTGVSTGVGSV
                     GLLKGGVVTATRVQIGAVGGGRVQILSGLSLGDTVVIGDASAPLPTNGGFGGFGGGLG
                     GAGGLTGGAVLVPGSNFGGGAPPGAPPAR"
     gene            complement(3064..>3609)
                     /locus_tag="PLB86_12415"
     CDS             complement(3064..>3609)
                     /locus_tag="PLB86_12415"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRC65992.1"
                     /translation="FGGLIRKSWQVLLVLSIVEIVLGIVVMAWPGATLRIVGVFFGIF
                     LVVSGISECVVGLSTPLMSGSFRLLNVIAGVLSFILGILCFRDGLGSLAVLGVWVGAG
                     WLMTGFSRLFTFGSLESMPGRGWAIAGAVITILAGIMAIVYPISSVVTLALLGGIALL
                     VVGIVGLVHAIQWKSTVNAIR"
BASE COUNT          514 a         1268 c         1252 g          475 t
ORIGIN      
        1 cagttcctcg tcgaggccag cctgctgggg ctcgtcggcg gcctggccgg gattgcgctg
       61 ggcctgttgg gcgctgcgat cctgcccaac gtcgtcagct tccccgtcgc catctcgccg
      121 gtcgccgccg ccgctgggct cggcgtctcg ctcgtcatcg gcatcaccgc cggcgtctat
      181 cccgccagcc gcgccgcgcg cctggccccc atcgaagccc ttcgcagtga ataggaaccc
      241 catgtcccgt atgccgcgca ccccccttct ccggttcgct cgaggcgccg ctgtgctcgc
      301 gtccgtcgct ctggcgggtt tcgcggtgac ggcctgcggc gctggggcgt caacgcccag
      361 cgccaccccg gccgtggcgg ccgcgacccg cacaggaggg cctggccccg gcgggggtca
      421 acggatcccc ggggtgtccg gactcatcgc tgccgtcgat gggacgacca tgcaggtcca
      481 gtcgagcacc aagcaaaccg ccgtcggttg gacggcaacg acgagcgtcg cccgaactgc
      541 cgcgggcacc ctcgcggaca tcgtcgctgg ttcgtgcgtc gtcgttcgca gcgcctcaag
      601 cgacggcagc gcgcccgacg cgggcggcgg cgtcaccgcc acttctgtgc aggtgtccgc
      661 ggccgtgaac ggcgcctgcg ccgacgtgtt tggcggcggc atccgaggtg gcggggggat
      721 tccctccggt atgccgaccg gccagcccgg tgtcgaccag ctgccgcccg gagcgaccga
      781 cggggcaccg gggggccccg gtggccgagt tccgggtggg ttcggggtcg ttggcctggt
      841 cacccaggtc ggctcgggga ccttcaccgt ggcgagcctg actcgcgccg gtgacggcgg
      901 ctcggggacg gcgtcggcgg cgccggccca gaccacgccc gtcacggtga caacgtccgc
      961 atcgacgacg gtgacgacca cgaaggtcgc cgcagccggc gatatcgcgg tggggctgtg
     1021 cgctaccgcg atcggaccca ccgacgacat cggcaaggtg acggccacct cgatcgtcct
     1081 gcgggatgcc gtcaacggcg cgtgcactag caccggtggg ttcgacgggc cgggtgcccc
     1141 cggcgcgggg ttcccgggcg gcggcggtcc cggtggcggc ggtcccggtg gcgtggcccc
     1201 gacctcggcg gccaccaatg gctgaacggg tccttcggat caagcggaga cgctggctgc
     1261 cgatggtcgg tgtcggtggg gcgatcttgc tcgtggccgt cggcggcacc tatgtcgcca
     1321 acggcgtcag cgcggggtcg gagacggccg cgttgcgcac cggtcaggtc accactggat
     1381 cgatcgagca gactctgacc ctcaccggat cggtgacccg ggtcagccag atgacggcgc
     1441 atttcccggt cgctggcacc gtgacgggcg tgtcggtcgc cgtcggtgac gtcgtcacgg
     1501 ccggccaggc gctcgcgacg ctggacacgg gcccgctcaa ccaggccgtc ctcgatgcca
     1561 agtccaccct tgaccaggcc aaagccacgc tggccaacga caccacgacg gcgtcgtcga
     1621 gctcgtcgag ctcgtccgcc tcgtcgtccg gcacgagctc cagcagcccg acgtcaagca
     1681 gcagccgcac aagcgcctcc tcgagcccgt cgaccagcgc aggcggcggt ggcggtggcg
     1741 gtggccagaa ccagacgacc agcgcgctgg ccgccgtggc cgctgcccag gcgttggcca
     1801 agaccgcctg cgccccggtg ttgcccgtcg cgacggccac tccgacgagc nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgacgagca ccgcgacctc gaccgccacg
     1981 tccacgcaga cggcgacctc gaccgtcacc tcgaccacga cgacctcgcc cccgacggcg
     2041 caggagattg ctgactgcct gggtgcgctc agcgcggtga ccgcggctca gcaggcggcc
     2101 ggcgtcgcac tcacctcgta tgccggttct ctcagtgccg ccgcggccca agccgccgcc
     2161 caagcggctg cgcaagccgc cgcccaggct gcggcccagg cccaggcggc cgcccaggcc
     2221 aaggcggctg ccgctgcggc ccaacaggcc gccgccgccg ttcgtagctc aagcgggacc
     2281 gtgtccgagg ccaagctcat caccgaccga gccaacgtca ccaacgccga ggccgccctg
     2341 gtgaaggccc agaccaacct cgcggccgcc acgctcacgg cccccatggc cgggcgggtg
     2401 ggcgcgatcg cgtgggcggt cggtgacacc gcgacgaccg cgaacggcat acagatcgtc
     2461 ggatccggtg ccgcgaaggt gaccattcag gtgcctgccg ccaacctcgc ggcgatcgcc
     2521 gtcgggcagg ccgcgcgcgt cacggtcacc ggcattccgg tgatggacgg caccgtctcg
     2581 caggtcgcgt tgctgcccac cagcacgacg ggctcgtcag cctcgacgta tgccgttgac
     2641 gtccttgtcc ccaacgcccc cgatgcggtc ggcacgggcg gcaaggcatc cgtcgccgtc
     2701 gtcacgacca aagtggacgg cgtcctcacc gtgcccgcgt cggcagtcac gggggtgtcg
     2761 accggggtcg ggtcggtcgg gctgctcaag ggcggcgtcg tcacggcgac ccgggtgcag
     2821 atcggtgcgg tgggtggcgg aagggtccag atcctctcgg gtctgtccct tggcgacacc
     2881 gtcgtcatcg gcgatgcgtc tgcgccgttg ccgaccaacg gtgggttcgg cggcttcggc
     2941 ggagggttgg gtggtgcggg tggcctcacg ggcggtgcgg tcctggtgcc aggctccaac
     3001 ttcggcggtg gggcaccccc aggagcgccg cccgcccgct gacccgaggc atggatcaga
     3061 cggtcagcgg atggcgttga ccgtgctctt ccattggatg gcgtggacga gcccgacgat
     3121 tcccacgacg agcagggcga taccaccgag cagcgcgagg gtcacgacgg acgagatcgg
     3181 gtagacgatc gccatgatgc ccgcgaggat cgtgatgacg gcgcccgcga tcgcccagcc
     3241 acggccgggc atgctctcga ggctgccgaa cgtgaagagg cgcgagaagc ccgtcatcaa
     3301 ccagccggca ccgacccaga cgccgagcac agccaggctg ccgagcccat cccggaagca
     3361 gaggatgccg aggatgaacg agagaacgcc ggcgatgacg ttgaggaggc ggaagcttcc
     3421 cgacatgagc ggggtcgaga ggccgacgac gcactcggag atcccgctga cgacgaggaa
     3481 gatcccgaag aagacgccga cgatgcgcag ggtcgctccg ggccaggcca tgacgacgat
     3541 gccgagaacg atctcgacga tgctcagcac gagcaggacc tgccacgact tacgaatgag
     3601 gccaccgaa
//
LOCUS       DAORPW010000231         3606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_8430_length_3606_cov_682.962264, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000231 DAORPW010000000
VERSION     DAORPW010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8430_length_3606_cov_682.962264"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLB86_12420"
     CDS             complement(<1..437)
                     /locus_tag="PLB86_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HRC65993.1"
                     /translation="MSLDDVAVGLVLGYAADLAFGDPRRWHPVAGFGRVAEALERHTY
                     APRRSSGIVHEAVLVGGAVALGQALTRLPRAARVPVVAAATWAVLGGRSLAREAEAMD
                     ALLQADDLAGARLRIRNLVGRDPSGLDADELARACVESVAENGA"
     gene            complement(482..1516)
                     /gene="cobC"
                     /locus_tag="PLB86_12425"
     CDS             complement(482..1516)
                     /gene="cobC"
                     /locus_tag="PLB86_12425"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2231c family pyridoxal phosphate-dependent
                     protein CobC"
                     /protein_id="HRC65994.1"
                     /translation="MTERLDHHGDAEVRGATHLVDLAVNVRVGTPPAWLATVIRDTTG
                     QLAAYPDPSVARAAVAHRHGVPEDMVLPTSGGAEAFTLIARAIAARRPLVVHPQFTEP
                     EAALRRAGRVPDRHVLQAKSGFTLDPAAVDPRADLVLVGNPTNPTGVLHPRATLEALR
                     APGRVLVVDEAFADAIRGERESLISPDLAGVLVLRSLTKTWGVAGLRAGYVVGDPDLV
                     AALAEQQSPWSVSTPAAAVMAATATPEALAEVARVAEAIARDRGHLVQALESIGLRPV
                     PGEAPFVLVEVGPGIHAELREQGYAVRRGDTFPGLDGGWVRIAVRDAATSDALVRALA
                     EVRLRGGLRR"
     assembly_gap    1675..1774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1775..3496
                     /locus_tag="PLB86_12430"
     CDS             <1775..3496
                     /locus_tag="PLB86_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC65995.1"
                     /translation="YDLGEYARPVTTTVAAAATWVNRGLVWTFGFNHEEAVECFQRAI
                     DLDPDCALAHWGIAFALGPNYNKPWEFFAPDEATAVLERARDEARVAADCASGGTAVE
                     QALTGALLARYPDGRPIEECTAWEVEYAAAMGQVYAAHGGDLDVACFYAEALMNLTAW
                     QLWDQRTGEPAEGSRTLEAKAVLEAALAVPGGSQHPGLLHMYIHLMEMSPTPERALPV
                     ADLLRGRVPDAGHLQHMPTHLDVLCGDYRSVVASNSAAITADAAFVARRGPLNFYTLY
                     RAHNLHFKIYGALFLGQERTALATVDQLEAAIPEELLRVQLPPMADWLEGFVPMRVHA
                     LVRFGRWDALIGLPLPADQDLYCTTTAMLHYGKGVAFSATGRVAEAEEERRLFLAAVE
                     RVPESRMLFNNTCRDILAVASEMLDGELEYRKGNHDSAFAHLRRSVELDDGLPYDEPW
                     GWMQPTRHALGALLLEQGHVEEAEAVYRADLGLDATLPRACQHPGNVWALHGYHECLV
                     RLAKHDLAEVVAQQLKLAGARADEPITASCLCRIDRSGALLAVDTCCSGEPPSGPAEP
                     ATGCCSH"
BASE COUNT          508 a         1205 c         1285 g          508 t
ORIGIN      
        1 gcgccgttct cggcgaccga ctcgacacac gcgcgggcca actcgtcggc atcgagccct
       61 gaggggtccc ggccgacgag gttgcggatc cgcagcctcg cgcccgccag gtcgtctgcc
      121 tgcagcaggg catccatggc ctcggcttcg cgggccagtg agcgccctcc gaggactgcc
      181 cacgtcgccg cggcaaccac cgggacccga gcagcccgcg ggagccgggt cagcgcctgc
      241 ccgagggcca ctgcgccccc gaccaacacc gcctcgtgaa cgatcccaga cgagcgccgc
      301 ggcgcatagg tatgccgttc gagcgcctcc gccactcgtc cgaacccggc gaccgggtgc
      361 cagcgccggg ggtcaccgaa ggcgaggtcc gcggcatacc cgaggacgag gccgaccgcg
      421 acgtcgtcca ggctcatggc gcctttcccc atcgagccgc gtccccggcc gccgatcccc
      481 gtcatcgccg cagacccccg cgaagccgga cctcggcgag ggcgcggacg agggcgtcgc
      541 tggtcgccgc gtcgcggacc gcgatccgca cccagccgcc gtcgagcccg gggaaggtgt
      601 cgccgcggcg cacggcatac ccctgttcgc gaagttccgc gtggatgccc gggccgacct
      661 cgacaaggac gaacggtgcc tcgcccggga cgggccgcag accgatcgac tcgagagcct
      721 ggactaggtg gccccggtca cgggctatcg cctcggcgac ccgcgcgacc tcggcgaggg
      781 cctcgggggt cgctgtcgcg gccatgacgg cggcggccgg ggtggacacc gaccaggggg
      841 actgctgctc ggcgagggcc gcgaccaggt ctggatcgcc gacgacgtaa cccgcgcgca
      901 gcccggcgac cccccacgtc ttggtgagcg agcggaggac gagaacgccc gccaggtcgg
      961 gactgatgag gctctcccgc tcgccgcgga tcgcgtcggc gaaggcctcg tcgacaacca
     1021 ggacgcgacc aggcgcgcgc agagcctcca gggtggcgcg cgggtgcagg acgccggtcg
     1081 ggttcgtggg gttgccgacg aggacgaggt cggcccgtgg gtcgacggcg gccggatcga
     1141 gggtgaagcc cgacttggcc tgcagcacat gccgatcggg cacgcggccg gcccggcgca
     1201 gggcggcctc gggctcggtg aactgcggat gcacgaccag tgggcgccgg gctgcgatgg
     1261 cgcgcgcgat cagcgtgaac gcctccgcgc caccactggt cggcaggacc atgtcctcgg
     1321 ggacgccgtg tcggtgggcg accgcggcgc gagcgaccga gggatctggg tatgccgcga
     1381 gctgcccggt cgtgtcccga atgaccgtcg cgagccaggc cggcggggtt ccgacccgga
     1441 cgttgacggc gaggtcgaca aggtgggtgg cccccctgac ctccgcgtcc ccgtggtggt
     1501 ccaggcgctc ggtcacctcg cgaccctatc ggtgtctccg aggcgtcgtc ctacactcac
     1561 gacgtgccct gcgtgtgtgg ccgaggcgcc cgatgggatc caccggccct caggtggtgg
     1621 accgcgagct gacctgccca gcgatggagg aactcatgac gacgaccggc ccgannnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntacgat ctgggcgaat acgcgcggcc
     1801 ggtgacgacc accgttgctg cggccgccac atgggtgaac cgcgggctgg tgtggacctt
     1861 cgggttcaac cacgaggagg cggtcgagtg tttccagcgg gccatcgacc tcgaccccga
     1921 ctgcgcactc gcgcactggg ggatcgcctt cgcgctcggc ccgaactaca acaagccgtg
     1981 ggagttcttc gcgcccgacg aggcgacggc ggtcctggaa cgtgcgcgcg atgaggcgcg
     2041 ggttgcggcg gactgcgcat cggggggcac ggcggtcgag caggcgctga ccggagcctt
     2101 gctggcccgg tatcccgacg gccgtccgat cgaggagtgc acggcctggg aggtggagta
     2161 tgccgccgcc atgggccagg tgtatgccgc gcatggcggc gacctcgacg tggcctgctt
     2221 ctacgccgag gcgctgatga acctcacggc gtggcagttg tgggatcaac gcaccgggga
     2281 gccggccgaa gggtcgcgaa ccctggaggc caaggcggtc cttgaggccg cgttggctgt
     2341 ccccgggggc tcgcagcatc cgggcctcct gcacatgtac atccacctga tggagatgtc
     2401 gccgaccccc gagagggcgc tgccggtcgc ggacctgctt cgaggacggg tgccggacgc
     2461 agggcatctg caacacatgc cgacccacct tgatgtgctg tgcggcgact accgcagcgt
     2521 ggtggcatcg aacagcgccg cgatcacggc cgatgctgcg tttgttgcgc gtcgggggcc
     2581 gctgaacttc tacacgcttt atcgggcgca caacctgcac ttcaagatct acggcgctct
     2641 gttcctcggg caggagcgga cggctctcgc cacggtcgat caactcgaag cggcgatccc
     2701 ggaggagttg ctgcgcgtgc agttgccgcc gatggccgac tggctggagg gcttcgtgcc
     2761 gatgcgggtg catgccctgg tccggttcgg tcggtgggat gcgttgatcg gcttgccgtt
     2821 gcccgcggac caggatctct actgcaccac cacggcgatg ctgcactacg gcaagggtgt
     2881 ggccttctcg gcgaccgggc gggtcgccga ggcggaggag gagcgtcggc tgttcttggc
     2941 tgccgtggag cgggtcccgg agtcgcggat gctgttcaac aacacctgcc gcgacatcct
     3001 ggccgtggca agcgagatgc tcgacggcga gctcgagtat cgaaaaggca accacgacag
     3061 tgcgtttgcg cacttgcgcc gttcggtcga gttggacgat ggcctgccct acgacgagcc
     3121 gtggggctgg atgcagccga cccggcacgc gctcggtgcg ctgctgttgg agcaagggca
     3181 cgtcgaggag gcggaggccg tctatcgcgc cgaccttggg ctcgacgcga ccttgccgag
     3241 ggcttgccag caccccggca acgtctgggc gctgcacggc tatcacgagt gcctggttcg
     3301 gcttgccaaa cacgacctcg ccgaggtcgt cgcgcagcaa ctgaagctgg ccggcgcccg
     3361 cgccgacgag ccgatcacgg cgtcgtgcct atgccggatc gatcgatcag gggcgctgct
     3421 ggcggtcgac acctgttgct ccggcgaacc tccttccggc cccgccgaac ccgcgaccgg
     3481 ctgttgctcc cactgacccc cccagcggtt gggcgccaag tcgttcactc atagggtggc
     3541 cgccatggag atgacggagc cggtgcgggc ggcagtggaa tcggcggtgc tgtgttggct
     3601 cgccac
//
LOCUS       DAORPW010000232         3567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_8585_length_3567_cov_962.392369, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000232 DAORPW010000000
VERSION     DAORPW010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8585_length_3567_cov_962.392369"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..307
                     /locus_tag="PLB86_12435"
     CDS             47..307
                     /locus_tag="PLB86_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRC65996.1"
                     /translation="MTTLAHRDLRNNSAEILRRAAAGESFEVTNNGRVVAMLVPPQVS
                     PLERLFLARLVTPPVARRLPHIARVQTTRSTAEILDDLKGER"
     gene            304..741
                     /locus_tag="PLB86_12440"
     CDS             304..741
                     /locus_tag="PLB86_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRC65997.1"
                     /translation="MTIHYVDASAWAKLLVEEPESGPLAAYVDERLAAGETFASSHLL
                     VTELHRLAARVGAAPTSVTAALSVLALTLPDAATFRAAGLLAGQVRSLDALHVASALD
                     LGVVDFVSYDQRQLAAAATAGIPAASPGTVPAAGPVADDGRVL"
     gene            complement(736..1701)
                     /locus_tag="PLB86_12445"
     CDS             complement(736..1701)
                     /locus_tag="PLB86_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRC65998.1"
                     /translation="MTEATPTPVQVLRGRLVPLGGIRAMYVRRTLPHRSRAFIGAWCF
                     VDHYGPNEIGADGSGGMDIPPHPHTGLQTVSWLFEGEIEHRDSGGAVGLVRPGEVNLM
                     TAGAGIAHTEVSTPETRRLHGVQLWVALPDAARDTHRNFEHHAPPLVPLRGVGSARVF
                     IGELAGVDRSPIHTFTPLLGAELILNPGAAVALAVDTAYEHGVLLDTGEVSVEGVPIE
                     SGDLACIDPGPDRLRLRAGAAGARIVLLGGVPFDEEIVMWWNFVGRSHDEILAYRQGW
                     EAGSIRFGTVKGYAGPVDRIPAPPLPAVRLRPRGRRGAGAEGISS"
     gene            complement(1794..3242)
                     /locus_tag="PLB86_12450"
     CDS             complement(1794..3242)
                     /locus_tag="PLB86_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HRC65999.1"
                     /translation="MTTHTFDAPQLDAPRGDLDQKPGARHTRSALLRRYQGQATRREV
                     RRDVLTVGAWSLIATSIALFLADNGIGYFATLGGAITALGIIAGLVATAFMCLMLVLT
                     ARVPVIDATLGQDRATSLHAKLGRGMVFGLLAHGILLVYGYALSDKITPIATFTQLWT
                     SSTDFIWACVAFLMLGVVGISSMVALRRKYPYEVWFGIHLTTYAAILASIPHQFSMSG
                     MLAEGTWGRPFWAAMWLVTGGLMLAYRVILPILASLTHQFTVSRVTPVGPDAVSIEVT
                     GRKVKELQARGGHWLNWRFLTPELAGQPHPFSLSAEPTDSTLRITVRNLGEGTARLMS
                     LKPGTKVAIEGPYGMFTDASRTTDKVVLLGAGIGIAPIRALLESTEFAPGDAAVILRA
                     STPDELYLLDEVVALCEAKGASLALLVGPRAGQSWVPVTDAGATLIDFVPWAAEADVY
                     VCGPNPWMEAVLADVAACGIPAAQVHDEKFDW"
     gene            complement(3361..>3567)
                     /locus_tag="PLB86_12455"
     CDS             complement(3361..>3567)
                     /locus_tag="PLB86_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66000.1"
                     /translation="VVLVVVPAAVVDETVFPLAVPTFQPTRRIPAEARAAAVLLRVLI
                     VVPSKRVERLALTTTLTTQAVREL"
BASE COUNT          567 a         1209 c         1285 g          506 t
ORIGIN      
        1 ccgctgggac tgaccccttg tcgtagcaca ttgctacagt gagcgtatga ccactttggc
       61 gcatcgcgac ctgcgcaaca acagcgccga gattctgcgt cgtgcggccg ctggcgagtc
      121 gttcgaggtg accaacaacg ggcgggtggt ggcgatgctc gtcccacccc aagtgtcacc
      181 gttggagcgc ctgttcctgg ctcgcctcgt cacgccgcct gttgctcgac ggctccccca
      241 catcgcgcgc gtccagacca cccggtcgac ggccgagatc ctcgacgacc tcaagggcga
      301 gcgatgacga tccactacgt cgacgcgtcg gcctgggcca agttgctcgt cgaggagccc
      361 gagagcgggc cgctcgcggc atacgtcgac gagcggctgg ccgcgggcga gaccttcgcc
      421 tcctcgcacc tcctggtcac cgagttgcac cggctggccg cccgggtcgg cgcagcgccg
      481 acgagcgtga cggcggccct gtcggtcctg gcgctgaccc tgccagatgc cgcgaccttc
      541 cgggcggcgg gattgctggc gggtcaggtc cgcagcctcg acgcgctgca tgtcgcctcg
      601 gcgctcgacc tcggagtggt cgacttcgtg agctacgacc agcgccaact cgcggccgcg
      661 gccacggcgg ggattccggc ggcaagccca gggactgttc cggcggcagg cccagtggct
      721 gatgacgggc gggtgctatg aactgatgcc ctcagcccca gcgcctcggc gaccacgcgg
      781 gcgaagccgc accgcgggca gcggtggcgc ggggatgcga tcgacgggac cggcataccc
      841 cttgacggtc ccgaaccgga tcgagcccgc ctcccacccc tgccgatagg cgaggatctc
      901 gtcgtggctg cggcccacga agttccacca catgacgatc tcctcatcga acggcacccc
      961 gccgaggagc acgatccgcg cgccggccgc ccctgcgcgc agtcgcagcc ggtcagggcc
     1021 ggggtcgatg caggcgaggt caccggactc gatcgggacg ccctcgacac tcacctctcc
     1081 ggtgtccagg agcacgccat gctcgtatgc cgtgtcgacc gcgagcgcga ccgccgcacc
     1141 tgggttgagg atgagttcgg cgccgagcag gggcgtgaag gtgtggatcg gcgagcgatc
     1201 caccccggcc aactccccga tgaacacgcg tgccgacccg acaccgcgca gcggcacgag
     1261 cgggggcgcg tggtgctcga agttgcggtg ggtgtcgcgc gccgcgtccg gcagggcgac
     1321 ccacagttgc acgccgtgca gccgcctcgt ctccggcgtc gagacctcgg tgtgggcgat
     1381 gccggcgccg gccgtcatga ggttgacctc gcccgggcgc acgaggccga ccgcgccgcc
     1441 ggagtcgcgg tgctcgatct cgccctcgaa gagccacgag acggtctgca ggccggtgtg
     1501 cgggtgcggc gggatgtcca tgccgccact gccgtcggca ccaatctcgt tggggccgta
     1561 gtgatccacg aagcaccagg cgccgatgaa ggcgcgggag cggtgcggga gggtgcgccg
     1621 cacgtacatc gcccggatgc cacccaaggg caccaaacga ccgcggagca cttggaccgg
     1681 tgtcggcgtc gcctcagtca ccggcccagt agagcacgcg gcccgggcgc gcgcacggct
     1741 gagggggcga cccggcatac cggatcgccc cctcgcccac gcggctggtg cggttaccag
     1801 tcgaacttct cgtcgtgcac ctgcgcggcg ggtatgccgc acgcggccac atcggcgagc
     1861 accgcctcca tccacgggtt gggtccgcag acgtagacgt cagcctcggc ggcccagggg
     1921 acgaagtcga tcagcgtggc gccggcgtcg gtcaccggca cccacgactg accagcgcgc
     1981 gggccgacga gcagcgcgag cgaggctccc ttggcttcgc acagtgcgac gacctcatcg
     2041 agcaggtaga gctcgtccgg ggtggacgct cgcaggatga ccgcggcgtc gccgggagcg
     2101 aactcggtcg actccagcag ggcccggatc ggggcgatcc cgatgccggc gccgagcagc
     2161 accaccttgt cggtcgtccg ggaggcgtcg gtgaacatcc cgtaggggcc ttcgatggcg
     2221 accttggtgc cgggcttgag cgacatgagg cgggccgtgc cctcgccgag gttgcggacg
     2281 gtgatccgca gcgtcgagtc ggtgggctcg gccgacagcg agaacgggtg cggttggccg
     2341 gcgagctcag gggtgaggaa gcgccagttg agccagtgcc ctccgcgcgc ctgcaactcc
     2401 ttgaccttgc gtccagtgac ctcgatcgag acggcgtccg ggccgaccgg ggtgacgcgg
     2461 ctgacggtga actggtgggt gaggctggcc aggatcggca ggatgacgcg ataggcgagc
     2521 atcaggccgc cagtgaccag ccacatcgcg gcccagaacg gccggcccca cgtgccttcg
     2581 gcgagcatcc cgctcatcga gaactggtgc gggatcgagg cgaggatcgc ggcatacgtg
     2641 gtcaggtgga tgccgaacca cacctcgtag gggtacttcc ggcgcagggc gaccatcgag
     2701 gagatgccga ccacgcccaa catgaggaag gcgacgcagg cccagatgaa gtcggtcgac
     2761 gacgtccaca actgggtgaa ggtcgcgatc ggcgtgatct tgtccgacag cgcgtagccg
     2821 tagacgagca ggattccgtg ggcaaggagc ccgaagacca tgccgcggcc gagtttggcg
     2881 tgcagcgagg tcgcgcggtc ctgcccgagg gtcgcgtcga tgaccggcac ccgtgcggtg
     2941 agcacgagca tgaggcacat gaaggcggtc gcgacgaggc cagcgatgat gccgagcgcg
     3001 gtgatcgcgc cgcccagcgt ggcgaagtag ccgatcccgt tgtcggcgag gaacagcgcg
     3061 atcgaggtcg cgatgagcga ccaggcgcca acggtgagga cgtcgcggcg cacctcgcgc
     3121 cgggtggcct gcccctggta gcggcgcagg agagcagatc gggtatgccg cgcgcccggc
     3181 ttctggtcga ggtccccgcg gggcgcgtcg agctggggag cgtcgaaggt gtgcgtggtc
     3241 atggccctta ccttccgggt cgggtctgtg cggttcttgt gagccgactt tgatgtcgat
     3301 ctgactcggt ggaagcgctt gcaaggagag gtcgggattc tcagccaggc ccctcggtgc
     3361 tcacaactcg cgcacagcct gggtcgtcag agtcgtcgtt aacgccagac gctccaccct
     3421 cttggaaggc acgacgatga gcacacgtag caagacagcg gccgccctag cctcagccgg
     3481 gatcctcctg gtcggctgga acgtcggcac ggccaacgga aagaccgtct cgtccaccac
     3541 ggccgcaggc accaccacga gcaccac
//
LOCUS       DAORPW010000233         3558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_8625_length_3558_cov_826.118470, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000233 DAORPW010000000
VERSION     DAORPW010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8625_length_3558_cov_826.118470"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="PLB86_12460"
     CDS             complement(<1..122)
                     /locus_tag="PLB86_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HRC66001.1"
                     /translation="MTGPDAGGAGEVGVGPYAGPPLSGDHWDPVLLAEGDRRNVV"
     gene            complement(119..853)
                     /locus_tag="PLB86_12465"
     CDS             complement(119..853)
                     /locus_tag="PLB86_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRC66002.1"
                     /translation="MSLLSVRGLRKSFGSNVVLDGFDLEVSAGECVVLIGASGSGKST
                     LLRCVNLLETVDDGVIHLGGEDITDPRVDPDRVRSRMGMVFQAYNLFPHLSVLDNITL
                     APMRVHGRPRADATAAARAILERVGLADKATVRPDELSGGQQQRVAIARALVNSPELM
                     LLDEVTSALDPELVGEVLDLLISLKAEGMTMVLATHEMAFAKQVADRVCFLHGGAIHE
                     EGPPAALLGAPSRERTRAFLSRFVGL"
     gene            complement(894..1760)
                     /locus_tag="PLB86_12470"
     CDS             complement(894..1760)
                     /locus_tag="PLB86_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRC66003.1"
                     /translation="MDAPGGLTDRWVPSALEQERRAYRRSRTVRSAAIASISTILVVG
                     ALGWVLTHSSGWPRVQETFFDWDKAVASFPAVLSGLWLNIRVLLVCAVLITVLGLTIA
                     VMRTLRGPVFFPLRLFGAAFTDLFRGLPLLLVLLLLGFGVPALGLDGLPQEAVFWGCA
                     ALVLSYSAYVAEVFRAGIESVHPSQRAAARSLGLTHTQSLRFVIVPQAVRRVLPPLLN
                     DFVSLQKDSGLIAVLGVVDAIRAAQIETARDFNFTPYVVAGVLFVCLTVPMARFTDWV
                     ARRQGFQGSGLV"
     gene            complement(1829..2737)
                     /locus_tag="PLB86_12475"
     CDS             complement(1829..2737)
                     /locus_tag="PLB86_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC66004.1"
                     /translation="MSKGTFMLRSRMLAVSGSALLLAGLAACGGSTSTGSTTSAVATK
                     ASSASATTDACAIASLPLTTAGKFTVGTDDPAYEPWFKDGKPANGQGYEAAVAAAVAA
                     KMGFAAADVQWVVVPFTNAITPGKKNFDVDINQVSISEERRKAVDFSSGYYDVAQAVI
                     TYKGSPIDGKTAVADLAGAKLGAQVGTTSYKAITDQIKPTAAPAVFDTNDLAVQALKN
                     KQIDGIVADVPTAFYMTAAQLDNGVIVGQLPAAGGAQEQFGLVLTKGSGLTACVSKAV
                     DALRADGTLAKLATQWLTNAGAPALK"
     gene            2920..3558
                     /locus_tag="PLB86_12480"
     CDS             2920..3558
                     /locus_tag="PLB86_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRC66005.1"
                     /translation="MPPALGPDWLDPNYLFETYGSGFFWLSLGIVFVECGLFFPFLPG
                     DSLLFAIGLFISGGKPHLPSLAIALPTLTVVAFLGNVCGYEIGRAMGPALYKRTGRIL
                     KQEYFDKTHAFFEKHGTKALVIGRFVPIVRTFITLVAGVSAMNRRTFFVWSAVGAVLW
                     VLSITLAGYFLGAAFPAIGKNLEIAVIAIIGVSLLPMVFEWWRHRRAAASAD"
BASE COUNT          563 a         1199 c         1267 g          529 t
ORIGIN      
        1 acgacgttgc gccggtcgcc ctcggcaagc agcacggggt cccaatgatc gccggagagg
       61 ggcggaccgg catacggacc gacgcccacc tcgcccgccc ctcccgcgtc ggggccggtc
      121 acaggccgac gaaccgggac aggaacgcgc gggtgcgctc gcgcgacggg gcgccgagca
      181 gggccgccgg cggaccttcc tcgtggatgg cgccgccgtg caggaagcac acccggtcgg
      241 ccacctgctt ggcgaaagcc atctcgtggg tggccagcac catcgtcatc ccctcggcct
      301 tgaggctgat gagcaggtcg agcacctcgc cgaccaactc cgggtcgagg gccgaggtca
      361 cctcgtcgag gagcatgagc tcgggtgagt tgaccagggc ccgggcaatg gcgacccgtt
      421 gctgctgccc gccggacaac tcgtcggggc gcaccgtcgc cttgtcggcc agcccgaccc
      481 gctcaaggat cgcccgggcg gcggcggtcg cgtcggcccg aggtctgccg tggacccgca
      541 tcggggcgag ggtgatgttg tccaggacgc tcaggtgcgg gaacaggttg taggcctgaa
      601 agaccatccc catccgcgac cgcacccggt cagggtcgac ccgcgggtcg gtgatgtcct
      661 cgccgccgag gtggatgacg ccgtcgtcga cggtctccaa caggttgacg caccgcaaca
      721 gggtcgactt gcccgacccg gaggcgccga tgaggacgac gcattcgccg gccgacacct
      781 ccaggtcgaa cccgtcgagg acgacgttgc tgccgaacga tttccgcaac ccgcggacgg
      841 acaggaggct cacggcatac ctcggtggcg ggagcggccg acggcggaac cggtcatacc
      901 agcccgctcc cctggaagcc ctggcggcgg gccacccagt cggtgaagcg cgccatgggg
      961 acggtgaggc agacgaaaag gacgccggcg acgacatacg gcgtgaagtt gaagtcgcgg
     1021 gccgtctcga tctgggccgc gcggatcgcg tcgacgacgc cgaggaccgc gatgagcccg
     1081 gagtccttct gcagggacac gaagtcgttg agcaacggcg gcagcacccg gcgcaccgcc
     1141 tgcggcacga tgacgaaccg cagggactgg gtgtgggtca ggccgagcga ccgggcggcc
     1201 gcgcgctgcg acggatgaac cgactcgatg cccgcgcgga agacctctgc gacataggcg
     1261 gagtagctga gcaccaatgc cgcgcagccc cagaacaccg cctcctgcgg caacccgtcc
     1321 agacccaacg cgggcacgcc gaaacccagc agcagcagga cgagcagcag cggcagaccc
     1381 cggaacaggt cggtgaacgc cgccccgaac agccgcaacg ggaagaagac cgggccgcgc
     1441 agggtccgca tgacggcgat ggtcagaccc aacaccgtga tgaggaccgc gcagaccagc
     1501 agcacccgga tgttgagcca tagcccgctc agcaccgccg ggaacgacgc gacggccttg
     1561 tcccaatcga agaacgtctc ctggacccgc ggccacccgg acgagtgcgt gagcacccac
     1621 ccgagcgcgc cgacgacgag gatcgtgctg attgacgcga tggccgccga ccgcaccgtc
     1681 cgcgagcggc ggtaggcgcg gcgctcctgc tccagcgcgg acggcaccca gcggtcggtc
     1741 agaccgccgg gtgcgtccac acgctcgtcg ggagggttcg ccacgaagat cccgaggggg
     1801 tatgccgtgt ggtgaggtga gcgggtggtt acttgagcgc cggggcgccg gcgttggtga
     1861 gccactgggt cgcgagcttg gccagggtgc cgtcggcccg cagcgcgtcg accgccttcg
     1921 agacgcaggc ggtcagcccg gaccccttgg tgaggaccag cccgaactgc tcctgcgcgc
     1981 cgccagccgc cggcaactgc ccgacgatga cgccgttgtc caactgcgcc gcggtcatgt
     2041 agaaggcggt cgggacgtcc gcgacgatgc cgtcgatctg cttgttcttc agcgcctgga
     2101 ccgccaggtc gttggtgtcg aacacggccg gggccgccgt cggcttgatc tggtcggtga
     2161 tcgccttgta ggaggtcgtg cccacctggg cgccgagctt ggcgccggcg aggtcggcca
     2221 cagccgtctt gccgtcgatc ggcgagccct tgtaggtgat gacggcttgg gcgacgtcgt
     2281 agtagccgga cgagaagtcc actgccttgc gacgctcctc ggagatcgac acctggttga
     2341 tgtcgacgtc gaagttcttc ttgccggggg tgatggcgtt cgtgaacggc acgacgaccc
     2401 actggacgtc ggcggcggcg aagcccatct tcgcggccac agcggcggcg acggccgcct
     2461 catagccctg cccgttggcc ggcttcccgt ccttgaacca gggctcgtat gccgggtcgt
     2521 cggttccgac ggtgaacttg cccgcggtgg tcagcggcag cgacgcaatg gcgcaggcat
     2581 cggtggtggc gctcgccgaa gaggccttgg tcgcaacggc cgacgtcgtc gatcccgtcg
     2641 aggtgctccc cccgcaggcg gccaggccgg ccagcaacag ggcggacccg gagacggcga
     2701 gcattcgcga gcgcaacatg aacgttccct tcgacatcga attcaaggcg ccgagatcgt
     2761 cccgaccccg gctgacaccc gtcatcgtag ggggccatcg gagcggcggt tgcttggtcc
     2821 gttgtttgtg gaaggcccgc gctggtgtcc gcgaacacgg cgcgcgcaca ggtgaggatt
     2881 acggggcagc caggcgggcg gcataccctc ggggtcgtga tgcctcctgc cctcggcccc
     2941 gactggctcg acccgaacta tctgttcgag acctacggga gcggattctt ctggctctcg
     3001 ctcggcattg tgttcgtcga gtgcgggctg ttcttcccgt tcctgcccgg cgactcgctg
     3061 ctgttcgcga ttgggctgtt catctcgggg ggcaagccgc acctgccctc gctggcgatc
     3121 gcgctgccga cgctcaccgt cgtggcgttc ctcgggaacg tgtgcgggta tgagatcggg
     3181 cgggcgatgg ggccggcgct ctacaagcgg acggggcgga tcctcaagca ggagtacttc
     3241 gacaagacgc acgccttctt cgagaagcac ggcacgaagg cgctggtcat tggccggttc
     3301 gtgccgatcg tgcggacctt catcaccctc gtcgccgggg tcagcgcgat gaaccggcgg
     3361 accttcttcg tgtggagcgc cgtcggcgcg gtgctgtggg tgctgtcgat cacgctcgcc
     3421 ggctacttcc ttggcgcggc gttcccggcg atcggcaaga acctcgagat cgcggtgatc
     3481 gccatcatcg gggtgtcact gctcccgatg gttttcgagt ggtggcgcca ccgccgggcc
     3541 gccgcgtcag ctgactga
//
LOCUS       DAORPW010000234         3511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_8812_length_3511_cov_875.412037, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000234 DAORPW010000000
VERSION     DAORPW010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8812_length_3511_cov_875.412037"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1014)
                     /locus_tag="PLB86_12485"
     CDS             complement(94..1014)
                     /locus_tag="PLB86_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HRC66006.1"
                     /translation="MQDPTALYRFENDADPAEVRAGVLVAALGGFVDAGGTQRLLTAH
                     LLENHSSQVVASFDVDQLLDYRGRRPTMTFDKDRWTAYDDPSLVLHRVVDRAGTPFLL
                     LAGPEPDYQWERVTAAILQLVAALGVQLTVSVHGIPMAVPHTRPLGTTPHATTGRLLG
                     EVEPVFGTIQVPGSIAGLLELRLGEAGRDAMGYAVHVPHYLAQADFPDASLVAFDALV
                     GATGLDLARDELATSALRTREQVATELADNDEVRTLIVSLERQYDAFMEGRTRPSLLA
                     TGMGEVPSADEIAADVEAFLREVNDEGRQA"
     gene            1152..1583
                     /locus_tag="PLB86_12490"
     CDS             1152..1583
                     /locus_tag="PLB86_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66007.1"
                     /translation="MTTPQDASPAPAPGAIAVTISLPWTIGVSIKPNVLLDGVRVVNA
                     AGRNVYPATPGQHLLSCEVNVRGSTFGQGDIAVDVQPGATTEVYYAAPLSANLPGRLG
                     LTPQEPYRGGAAGCLIAAAIGFLVLFVFWVVIGGFLLLGPR"
     gene            complement(1592..>3511)
                     /locus_tag="PLB86_12495"
     CDS             complement(1592..>3511)
                     /locus_tag="PLB86_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HRC66008.1"
                     /translation="VMAGELVETTRLWAHDVARIDPVWAEELGSHLVKRQYSEPRWSS
                     RQGSAVATERVTLYGVPLVVGRTVGLAKVDPALARELFIRHALVEGDWTTHHRFFHDN
                     RALLERLSELEARTRRRDILVGDDVLQDFYEHRLPAEVVSARHFDSWWKRARRTTPDL
                     LTFTEDLLVRDEAGDIGADAFPTTWHQGDLDLPLTYQFEPGAAADGVTVHVGTEILNR
                     VAADGFDWQVPGLREELVTALIRTLPKTTRRLLVPAPEHAAATVRELRQRGLGPADGP
                     LTAVLAQVLRETHGVVIRPDDWDIARLPDHLRVTFAVEDRPGHAIAAGKDLAALREQA
                     APQVRRQVAAAGRAITRTGMRTWAIDDVPLEHDAVVSGVRVHGFPALVDEGDAVALRV
                     LPTPAEAESEHRLGVRRLLLLGTTPPWKHVLGRLTNAQKLALGHNPHGSVPALLDDVL
                     AAAVDAIAAERVHHVPRTAAEFAAAQDAVRTHLGARVVAAVGLVEPILAKHLQIVRRL
                     DGMTAPHLADLRADVRAQLAELIRPGFVADTGLARLPDLDRYLRAVLHRLDKAPLDLA
                     RDSQRLTEVLDAEAGYAALLASLRPSQRGAAQVTDIAWVIEELRVSLFAQSLGTAYPI
                     SVKRIEKAIGAIPRG"
BASE COUNT          521 a         1212 c         1278 g          500 t
ORIGIN      
        1 gccaagggct gatcgccccc gcctcagccc gatcgcgatc tgcggagtgt tagccacatt
       61 ccgatgtggc caacactccg cagatcgggt cgatcaggcc tggcggcctt cgtcgttgac
      121 ttcgcgcagg aacgcctcga catcagcggc gatctcgtcg gcagaaggca cttcgcccat
      181 cccggtcgcc aacaggctcg gtcgggtgcg gccctccata aacgcgtcgt actgccgctc
      241 aagcgacacg atgagcgtcc ggacctcgtc gttgtccgcc aactccgtgg cgacctgctc
      301 acgggtacgc agggcagagg tcgccaactc gtcgcgcgcc agatcgagac cggtcgcgcc
      361 gacgagggcg tcgaaggcca cgagcgaggc gtcggggaag tcggcctggg cgaggtagtg
      421 cgggacgtgg acggcatacc ccatggcgtc gcggcccgcc tcgccgaggc gaagttcgag
      481 cagtccggcg atgctcccgg gcacctggat cgtcccgaag acgggctcga cctcgccgag
      541 gaggcggccg gtggtcgcgt gcggggtcgt gcccagcggg cgggtgtgcg ggacggccat
      601 gggtatgccg tgcacgctca ccgtcagttg gacgccgagg gctgcgacca actgcaggat
      661 cgcggcggtg acccgctccc actggtagtc gggctcggga ccggcgagca ggaggaacgg
      721 cgttccggcc cggtcgacca cgcggtgcag gaccagcgag gggtcgtcgt aggcggtcca
      781 gcgatccttg tcgaaggtca tcgtcggacg ccgcccgcgg tagtcgagca actggtcgac
      841 gtcgaaactc gcgacaacct gggacgagtg gttctccagg aggtgggcgg tgagcaacct
      901 ctgggtgccc ccggcatcga cgaaaccgcc cagggcggca acgagcacac cagccctcac
      961 ctcggcgggg tcggcgtcgt tctcgaatcg gtagagcgct gtcgggtctt gcacgtactc
     1021 atcctcccaa gtgctgatgt cgcgtcaaca cgcagggcgc ccgagccatt ccgagcgggc
     1081 cgccgcgaag ggcggtcgcg gccagcccta ggctgggaac gactcgccgg ctccgcgctc
     1141 caagggacca catgaccact ccgcaggacg ccagccccgc accggcgccg ggcgccatcg
     1201 ccgtgaccat cagcctgccg tggacgatcg gcgtgtcgat caaaccgaac gtcctgctcg
     1261 acggcgtccg cgtggtcaat gccgccggcc gcaacgtcta tcccgcgacg cccggccagc
     1321 acctgctctc gtgcgaggtc aacgtccgcg ggtccacctt cgggcagggc gacatcgccg
     1381 tggacgtcca accgggagcg accacagagg tctactacgc cgcaccgctg tcggccaacc
     1441 tgcccggtcg cctaggcctc acgccccagg agccctaccg cgggggtgct gccggttgcc
     1501 tcatcgccgc cgcgatcggg ttcctcgtgc tcttcgtctt ctgggtcgtc atcggcgggt
     1561 tcctgctgct cggcccacgc tgagcggcgc gtcagccgcg agggatcgcg ccgatcgcct
     1621 tctcgatccg cttgaccgag atggggtatg ccgtcccaag actctgcgcg aacagcgaca
     1681 cccgcagctc ttcgatcacc cacgcgatgt ccgtgacctg ggccgccccc cgttgggagg
     1741 ggcgcagcga agccagcagc gcggcatacc cggcctcggc gtcgagcacc tcagtgagcc
     1801 gctggctgtc gcgggccaga tccagcggag ccttgtccag gcgatgcagg acggctcgca
     1861 ggtagcggtc gaggtcgggc agccgggcca ggccggtgtc ggcgacgaag cccggccgga
     1921 tgagctcagc gagttgggct cgcacgtccg cacgcaggtc ggccaggtgc ggcgcggtca
     1981 tgccgtcgag gcggcgcacg atctgcagat gcttggcgag gatcggctcg acgaggccga
     2041 ccgcggccac gacccgggcg cccaggtggg tccggaccgc gtcctgggcg gcagcgaact
     2101 cggcggcggt gcgcgggacg tgatggaccc gttcggcggc tatcgcatcc acggcggcgg
     2161 cgaggacgtc atcgagcagc gccgggaccg agccgtgtgg attgtggccg agcgccagtt
     2221 tctgcgcgtt ggtgagccgc ccgaggacgt gtttccacgg cggtgtggtg ccgagcagga
     2281 gcagccgccg caccccgagg cggtgttcgg actcggcctc ggcaggggtg ggcagcaccc
     2341 gcagggcgac ggcgtccccc tcgtcgacga gggccgggaa gccgtggacg cgcacccctg
     2401 acacgacggc atcgtgttcg agcggcacgt cgtcgatcgc ccacgtgcgc atgcccgttc
     2461 gcgtgatggc gcgaccggcg gccgccacct gccggcgcac ctgaggggcg gcctgttcgc
     2521 gcagggcggc caggtccttg cccgcggcga tggcgtgccc ggggcggtcc tccaccgcga
     2581 acgtcactcg caggtggtca ggcagccggg cgatgtccca gtcgtcgggg cggatgacga
     2641 cgccgtgggt ctcacgcagc acctgggcca ggacggcggt gagcggaccg tcggccggcc
     2701 cgagcccacg ctggcgcaac tcccggaccg tcgcggcggc gtgctccgga gccggcacga
     2761 gcaaccgacg ggtcgtcttg ggcagcgtgc ggatgagtgc ggtgaccaac tcctcgcgca
     2821 atcccggcac ctgccagtcg aatccgtccg cagcgacccg gttgagaatc tccgtgccga
     2881 cgtgcacggt gactccgtca gccgccgcgc cgggctcgaa ctggtaggtc aacggcaggt
     2941 cgaggtcgcc ctggtgccaa gtcgtcggga aggcgtcggc gccgatgtcg ccggcctcgt
     3001 cgcgcaccag caggtcctcg gtgaaggtga gcaggtcggg agtggtgcgc cgggcccgct
     3061 tccaccacga gtcgaagtgg cgggccgaga cgacctccgc agggagccga tgctcgtaga
     3121 agtcctgcag gacgtcgtcg ccgacgagga tgtcgcgacg ccgggtgcga gcctcgagct
     3181 ccgagagtcg ctcgagcagt gcgcggttgt cgtggaagaa ccggtggtgg gtcgtccaat
     3241 ccccctcgac gagggcatgg cggatgaaca gctcccgggc cagcgccggg tcgaccttgg
     3301 ccagcccgac ggttcggccg acgacgagcg ggacgccata gagagtgacc cgttcggtgg
     3361 cgacagccga gccctgccgc gaggaccagc ggggctcgga gtactgccgt ttgaccagat
     3421 ggctgccgag ctcctcggcc cacaccgggt cgatccgcgc gacgtcgtga gcccagagcc
     3481 gggtcgtctc gacgagttcg ccggccatga c
//
LOCUS       DAORPW010000235         3507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_8832_length_3507_cov_804.154983, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000235 DAORPW010000000
VERSION     DAORPW010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8832_length_3507_cov_804.154983"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /gene="rplI"
                     /locus_tag="PLB86_12500"
     CDS             <1..273
                     /gene="rplI"
                     /locus_tag="PLB86_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRC66009.1"
                     /translation="IKSLDDAKAVKSRIEGTTVTLSAKAGNSGRLFGAVSGSDLAAAV
                     VKAGGPELDKRKIEVPGHIKTVGSYVALVRLHPEVQAKLAFEVVKA"
     gene            complement(374..1471)
                     /locus_tag="PLB86_12505"
     CDS             complement(374..1471)
                     /locus_tag="PLB86_12505"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HRC66010.1"
                     /translation="MSPLTLLAAPLVVAGVFLLSGVAKLGDVSDGVRGLREMGVPDVF
                     VRDWVARAHPIAEIIIALGLLVLPTPWRVVAGVAAVGLMAAYTVLVIAAVRRAREVNC
                     NCFGRRSEVVNRRTAVRNLALLALALVALVDCLVPSAPIVRMFSGLDPLAWSVVLVVT
                     AGVAWLIGRGYAASAPTEPVPALSPILGGHHHEGNGESNGDDEDDEDVERDAIPDHTL
                     TAADGTTITLMELVAQRATVLLFMEPGCGPCGVLMAEVPSWRAIMPQITLTAVRLRPV
                     PGAKGLHEHAGPWQGTGYGTEPGTGEPFPTLEGGYWADPSIIQAFEIQGMTPWAVLLG
                     ADGLIAGGPVASYPHVRAFLVEILAHLAGLA"
     gene            complement(1591..2940)
                     /locus_tag="PLB86_12510"
     CDS             complement(1591..2940)
                     /locus_tag="PLB86_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRC66011.1"
                     /translation="MATAPPANPGDARVDRREIIGLAVPAFLALVAEPIFLAADSAIV
                     GHLGTIPLAGLGVAGSVLMAAAGIFVFLAYATTSVVARAMGAGSPTTAITAGLDGLWL
                     ALGLGAIAAAVVAAAADPLTAAFGASAAAQEQATTYLRIGALGLPPMLVLLAVTGALR
                     GLLDTRTPMLVAVAGFAVNIALNLAFVHGFHWGIAGSAWGTVIAQTGMALALTAVLLR
                     KARATHAPLRPHPGRVLAAARNGIPLVVRTLALRGAILLTVWAAAVLGDAPLAAHQIA
                     ATVWSFLAFALDALAIAAQAVVGRDLGAGRVDTARAVMRTLLRWGLLFGLAVGALTAA
                     AAGLVPRLFSSDPAVIGYAVPALLVVAAGQVVAGYVFVADGVLMGAGDFRYLAWAMVV
                     ALAAYAPVVLFVRALGVWGEPGRVLVWLWGGYTAFMLARAVSLGWRLRGDRWLIPGS"
     gene            complement(2968..>3507)
                     /locus_tag="PLB86_12515"
     CDS             complement(2968..>3507)
                     /locus_tag="PLB86_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRC66012.1"
                     /translation="PEWEATVRALWDEATHREHWYAALALLQHRHYRAWRDPDLLPLV
                     RHLVVSGAWWDVVDDIATHVVRDLLLGWPEPIATVLRAWSRDDHLWIRRVAMLAQVGA
                     GARTDPVLLADVLTPNIGYAGEQVFFSRKAIGWALRDYAWTAPDWVRAYVADHPDLSG
                     LSRREALKHLSRPTDAPTT"
BASE COUNT          571 a         1185 c         1317 g          434 t
ORIGIN      
        1 atcaagtcgc tcgacgacgc caaggccgtc aagtcccgca tcgagggcac gaccgtcacg
       61 ctgtcggcga aggccggcaa ctctggtcgc ctcttcggcg cggtgagcgg gtcggacctg
      121 gccgccgcgg tcgtcaaggc cggtgggccc gagctcgaca agcgcaagat cgaggttccc
      181 ggccacatca agacggtcgg ctcgtatgtc gcgctggtgc ggctgcaccc cgaggtgcag
      241 gccaagctcg ccttcgaggt cgtcaaggcc tgagtttttc tgccagtttc tctgccggat
      301 cgaggtattc ggccatcacc tgctgatggc tgaatacctc gatccggttt tgagttgggg
      361 tcccggcaag aggtcaagcc aagccggcga ggtgggccag gatctcgacg aggaacgcgc
      421 gcacgtgggg gtagctggcc accggtcctc ccgcgatcag cccgtcggcg ccaagcagca
      481 ccgcccacgg cgtcatgccc tggatctcga acgcttggat gatcgacgga tcggcccagt
      541 agccgccctc gagcgtcggg aagggctcgc cggtgccggg ctcggtcccg tatccggtcc
      601 cctgccaggg cccggcgtgc tcgtggagac ccttggcacc gggtacgggt ctgaggcgga
      661 cggcggtgag ggtgatctgg ggcatgatgg cgcgccagct cggcacctcg gccatcaaga
      721 cgccgcacgg cccgcacccc ggctccataa acagcaagac cgtcgcgcgc tgagcgacga
      781 gctccatgag cgtgatcgtc gttccgtcgg ctgcggtgag ggtgtggtcg gggatcgcgt
      841 cgcgttcgac gtcctcgtcg tcctcgtcgt cgccgttgct ttcgccgttg ccctcgtggt
      901 ggtgtccgcc gaggatgggg ctcagggcgg ggacgggttc ggtcggtgcg ctcgcggcat
      961 accctcgccc gatgagccag gcgaccccgg cggtgacgac gaggacgaca ctccacgcca
     1021 gggggtcaag tccgctgaac atccgcacga tgggtgccga cggcacgagg cagtcgacga
     1081 gcgcgacgag cgcgagcgcc aagagagcga ggttgcgcac ggcggtgcgg cggttgacga
     1141 cctcacttcg gcggccgaaa cagttgcagt tcacctcgcg cgcccgccgg accgcggcga
     1201 tcaccagcac ggtgtatgcc gccatcagcc cgactgcggc caccccggcc accacgcgcc
     1261 agggcgtcgg caggacgagc agcccgaggg cgatgatgat ctcggcgatc ggatgcgcgc
     1321 gagcgaccca gtcacgcacg aagacgtcgg gaacgcccat ctcgcgcagc ccgcgcaccc
     1381 cgtcgctgac gtcgccgagc ttggcaaccc cactgaggag gaacacgccg gcgacgacga
     1441 gcggggcggc gagcaaggtc aacggggaca ctcggtcacc ctatccgcgc cccctggctc
     1501 cctctgccca gtcgaggtat ttcgccatca tcgggcgatg gcggaatacc tcgattcggc
     1561 aggggatcag cttggggggt cgggcagggg tcaggagccg gggatgagcc agcggtctcc
     1621 gcgcagtcgc caacccaggc tcaccgcgcg cgccagcatg aacgccgtgt agccgcccca
     1681 caaccacacg agcacccgcc ccggctcgcc ccacacgccc agcgcgcgca caaacaacac
     1741 gaccggcgcg tatgccgcca gcgccacgac catggcccac gcgagatagc ggaagtcccc
     1801 ggcgcccatc aacaccccgt cggcgacgaa gacgtagcca gccaccacct gaccggcggc
     1861 gacgacgagg agggcgggga cggcataccc gatgacggcg gggtcggagg agaacaggcg
     1921 cggcaccagg cccgccgcgg cagccgtgag cgcgccgacg gcgaggccga agagcagccc
     1981 ccagcgcagc agggtccgca tcaccgcgcg ggccgtgtcg acgcgcccgg cgccgaggtc
     2041 gcgcccgacg acggcctgcg cggcgatcgc gagggcgtcg agcgcgaacg cgaggaagct
     2101 ccacaccgtc gcggcgatct ggtgcgcggc cagcggcgcg tcaccgagga cggccgccgc
     2161 ccacacggtc agcaggatcg ctccccgcag cgcgagggtc cgcacgacga ggggtatgcc
     2221 gttgcgcgcg gccgcgagga cccgccccgg atgggggcgc aagggggcgt gggtggctcg
     2281 ggccttccgc agcaggacgg cggtgagggc gagcgccatg ccggtctggg cgatgaccgt
     2341 gccccacgcg gatccggcga tgccccaatg gaatccgtgg acgaaggcca ggttgagggc
     2401 gatgttgacg gcgaatccgg cgacggcgac gagcatgggg gttcgggtgt cgagcaggcc
     2461 gcgcagagcg cccgtgacgg cgagcaggac cagcatcggg gggaggccga gggcgccgat
     2521 ccgcaggtag gtggtggctt gctcctgggc tgcggcggag gcaccgaacg cggccgtgag
     2581 ggggtcggcc gcggcagcaa cgacggcggc cgcgatcgcg ccgagaccga gggcgagcca
     2641 gagcccgtcg agcccggccg tgatggcagt ggtgggggag ccggcgccca tggctcgggc
     2701 gacgacgctt gtcgtggcgt aggcgaggaa gacgaagatg ccggcggcgg ccatgaggac
     2761 cgaccccgcg accccgaggc cggccagcgg gatggtgccg aggtgtccga cgatcgccga
     2821 gtcggcggcc aggaagatcg gctcagcgac aagggcgagg aaggcaggga cggccagccc
     2881 gatgatctcc cgccggtcga ctcgtgcgtc gccggggttc gcaggcggtg ccgtggccac
     2941 gtcggtgacc ttagcggctg gatgcgttca ggtggtcggg gcgtccgtgg gtcgcgagag
     3001 gtgcttgagc gcttcgcggc gggacagccc ggacaggtct gggtgatcgg cgacataggc
     3061 ccgcacccag tcgggcgccg tccaggcgta atcgcgcagc gcccagccga tcgccttgcg
     3121 ggagaagaag acctgctcac cggcataccc gatgttgggc gtgaggacgt cggcgagcag
     3181 cacaggatcg gtgcgagcgc ccgcaccgac ctgggcgagc atcgcgacgc gacggatcca
     3241 caggtgatcg tcgcgggacc acgcgcgcag gaccgtggcg atcggctccg gccagccgag
     3301 cagcaggtca cgcacgacgt gggtcgcgat gtcgtcgacg acatcccacc acgcaccact
     3361 gacaacgagg tgacgcacca gcggcagcag gtcgggatcg cgccacgcgc ggtagtgccg
     3421 gtgctgcagg agggcgagcg cggcatacca gtgctcgcgg tgggtcgcct cgtcccagag
     3481 cgcgcggacg gtcgcctccc actcggg
//
LOCUS       DAORPW010000236         3468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_8995_length_3468_cov_324.735130, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000236 DAORPW010000000
VERSION     DAORPW010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8995_length_3468_cov_324.735130"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLB86_12520"
     CDS             complement(<1..694)
                     /locus_tag="PLB86_12520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HRC66013.1"
                     /translation="MERTLADARRWVAHGTHVCRDAIASLDEASFAQTCLLPGWTRKH
                     LVAHLAANGDAVRNLATWAATGVPTPMYSSPAQRSSDIEAGSQYVGSALTAWFDRSAQ
                     GLQEAWDGLSASAWQAPVVTAQGRTVPASETPWMRAREVLVHAVDLGTGVTFADLPSD
                     FLEALGDDITTKRAAAPMADSPAVEICPTDATGVCWTLPGSDLSTPITVAGPLAACTA
                     YLAGRGDSHLSAS"
     gene            complement(694..1539)
                     /locus_tag="PLB86_12525"
     CDS             complement(694..1539)
                     /locus_tag="PLB86_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRC66014.1"
                     /translation="MKLATIRLADGHTAAVRVDGDTLVHLGATDVGALLAQPEWRSRA
                     EAVTGTTSYAAADATFATLVPAPSKVVCVGLNYKTHIQEMGRDLPEYPTLFSKFADCL
                     IGPTDDIQRPAETTEFDWEVELAVVIGTTVRRATTEQAEAAIAGFTVLNDITCRDWQF
                     RTREWLQGKNWDSSTPVGPYLVTPDELPGGVRPAVAVRCEVDGEVVQSDTTGDLLFDP
                     VHLVEYVSTMVRLNPGDIIATGTPGGVGHARKPPRYLTGGEVIVTEIEGVGRLVNRVV
                     NEVAS"
     gene            complement(1536..2684)
                     /locus_tag="PLB86_12530"
     CDS             complement(1536..2684)
                     /locus_tag="PLB86_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC66015.1"
                     /translation="MNQPSVISPVHLSMGSSNTEPEPSAELADLYRGFEKELLVPLWT
                     EIGDLMPHTPKSKAQPHLWEWAKLLELAGLAGDLVPVGRGGERRAIALANPSLGGRPY
                     ATPTLWAAIQYLMPGEDAPEHRHTQNAFRFVVEGTGVWTVVERDPIPMNRGDFLPQFA
                     WNWHAHHNAADRPMAWIDGLDIPFQHYVESTFFDFGRDSISQAEHATPDRSRSQRLWG
                     HPGIAPLSQLGPTPGSPLLCYRWVDTDAALTDQLELEREGYAATLEPGHAAIRFLNPS
                     NASDVTQTIRTQFHRITSGTETAPRRQTGSSVYQVFDGSGRVTVGDFSWTVGRGDMFV
                     VPSWFPLSIRSEASASDSDSGALDLFEFGDAPIFEKLNLARTLVEDNR"
     gene            2846..>3468
                     /locus_tag="PLB86_12535"
     CDS             2846..>3468
                     /locus_tag="PLB86_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC66016.1"
                     /translation="MVIRSRPATARTAKTRPAYAIDSVDHALRLAAMLQLEGALTVSE
                     AADRLGVARSTAHRVLAMLVYRDFAVQGADRRYHAGPVLELATHSRSLTARLRAVSLP
                     HLEGLVAVLGESANLMIRTGDRVRFIASAESSQALRVEQREGMVFPAHLVTGGLVLLA
                     EAGAAEVDALYAAGPGREADTAGSVGRLGSVGGPGSVGGSGSVGGGP"
BASE COUNT          483 a         1084 c         1370 g          531 t
ORIGIN      
        1 ggctcgcgct caggtgcgag tcgccacgac cggcgaggta tgccgtgcac gcagcgagcg
       61 gtccggcgac ggtgatcggg gtgctgaggt cggatccggg caaggtccag caaacgcctg
      121 tggcatccgt cggacagatc tcgaccgccg gcgagtcggc catcggagcg gcggctcgct
      181 tggtggtgat gtcgtccccg agggcttcga ggaagtcgct cggcaggtcg gcgaaggtca
      241 cgcccgtgcc gaggtcgacg gcgtgcacga gcacctcgcg ggcccgcatc cacggggtct
      301 ccgaggccgg gaccgtccgc ccctgggccg tcacgacggg cgcctgccag gccgatgcgc
      361 tgagcccgtc ccaggcttcc tggagaccct gggccgagcg gtcgaaccag gcggtcagtg
      421 ccgagccgac atattggctg cccgcctcga tgtccgaaga ccgttgcgcg ggagaggaat
      481 acatcggagt gggcacccct gtggcagccc acgttgcgag gttgcggacg gcatcaccgt
      541 tggccgccag gtgcgcgacc aggtgtttgc gggtccagcc cggcagcaag caggtctgcg
      601 cgaaggacgc ctcgtcgagc gaggcgatgg catcgcggca tacatgggtg ccgtgcgcga
      661 cccagcggcg cgcgtcggcg agggtgcgct ccatcaggat gcgacctcgt tgaccacccg
      721 gttgaccagc cgaccgacgc cctcgatctc ggtgacgatc acctcgccgc cggtcaggta
      781 gcgcgggggc ttgcgggcgt gcccgacccc gccgggggtg ccggtcgcga tgatgtcgcc
      841 ggggttgagc cggaccatcg tcgagacgta ttcgacgagg tgcaccgggt cgaagagcag
      901 gtcgcccgtg gtgtcggact ggacgacctc gccgtcgacc tcgcagcgga ccgcgaccgc
      961 ggggcggacg ccgccgggca gctcgtcggg ggtgacgagg taggggccga ccggcgtcga
     1021 ggagtcccag ttcttgccct gcagccattc gcgggtgcgg aactgccagt cgcggcaggt
     1081 gatgtcgttg aggacggtga acccggcgat cgcggcctcg gcttgctcgg tcgtcgctcg
     1141 gcggaccgtg gtgccgatga cgacggccag ctcgacctcc cagtcgaact cggtcgtctc
     1201 ggccgggcgc tggatgtcgt cggtcgggcc gatgaggcag tcggcgaatt tcgagaagag
     1261 cgtggggtat tcgggtaggt cccggcccat ctcctggatg tgggtcttgt agttgaggcc
     1321 cacacagacg accttggagg gcgccgggac gagcgtcgcg aaggtggcgt cagcggcggc
     1381 atacgacgtc gtgccggtga ccgcctcggc ccgtgagcgc cactcgggtt gggcgagcag
     1441 cgcgccgaca tcggtcgcgc cgaggtggac gagcgtgtcc ccgtcgacgc ggacggcggc
     1501 agtgtgaccg tcggccaggc ggatggtcgc gagcttcatc ggttgtcctc cacgagggtg
     1561 cgggcgaggt tgagcttctc gaaaatgggt gcatcgccga actcgaagag atcgagcgcg
     1621 ccggagtcgg agtcggacgc agatgcctcg gagcggatcg acagcgggaa ccacgacggc
     1681 acgacgaaca tgtcaccgcg ccccacggtc caggagaagt cgccgacggt tacccggccc
     1741 gagccgtcga agacctgata gaccgacgaa ccggtctggc ggcgcggggc cgtctcggtg
     1801 ccggaggtga tccggtggaa ctgggtgcgg atcgtctggg tgacgtcgga ggcgttgctc
     1861 gggttgagga agcggatggc ggcgtggccg ggttcgagcg tcgcggcata cccctcgcgc
     1921 tccagctcga gctggtcggt gagggcggcg tcggtgtcga cccagcggta gcacagcagc
     1981 ggtgagccgg gggtcgggcc gagctgcgag agcggcgcga tgcccgggtg accccagagg
     2041 cgctgcgagc gcgagcgatc gggcgtggcg tgctcagcct ggctgatcga gtcgcggccg
     2101 aagtcgaaga acgtgctctc gacgtagtgc tggaagggga tgtccagccc gtcgatccac
     2161 gccatcgggc ggtcggcggc gttgtggtgg gcgtgccagt tccaggcaaa ctgcgggagg
     2221 aagtcgccgc ggttcatcgg gatcgggtcg cgctcgacga ccgtccacac gccggtgccc
     2281 tcgacgacga agcggaaggc gttctgggtg tggcggtgct cgggggcgtc ctcgccgggc
     2341 atgaggtatt ggatcgccgc ccacagcgtc ggggtggcgt aggggcggcc gccgaggctc
     2401 gggttggcca gcgcgatcgc ccggcgctca ccgccgcggc cgaccgggac gaggtcgccg
     2461 gcgaggcccg cgagctcgag cagcttggcc cactcccaca ggtgcggctg cgccttggac
     2521 ttgggcgtgt gcggcatgag gtcgccgatc tcggtccaga gcgggacgag cagttccttc
     2581 tcgaaacccc ggtagaggtc ggcgagttcg gccgacggct ccggctcggt gttgctgctg
     2641 cccatgctca ggtggacagg ggagatgacc gatggctggt tcatgagcgg ctccttggat
     2701 cctctctgat ggtgcccgcg atggcgtcgg cacatcgtcg cccggttgcg ccctggggaa
     2761 taggttgttc tgtctgacag aatggtgacc ggagcaacag ttccggaggg gagactcgcg
     2821 ccgtggtgaa acccgtggtg ttcccgtggt gatcaggagc cggccggcaa ccgcgcgcac
     2881 ggccaagacg cgaccggcat acgccatcga cagcgtcgac cacgcgctgc ggctcgcggc
     2941 gatgctgcag ttggaaggcg cgctcaccgt gagtgaggcc gccgaccggc tcggggtggc
     3001 gcgctcgacc gcgcaccggg tgctggcgat gctcgtctat cgggattttg cggtgcaggg
     3061 ggcagaccgg cgttaccacg cagggccggt gctcgagctg gccacccact cgcggtcgct
     3121 gacggctcgg ctgcgggcgg tctcgctgcc gcacctggag gggctggtcg ccgtgctcgg
     3181 cgagtcggcc aacctgatga tccgcaccgg cgaccgggtg cggttcatcg cctcggccga
     3241 gtccagccag gcgctgcggg tcgaacagcg cgaggggatg gtcttcccgg cgcacctggt
     3301 cacgggtggg ctcgtgctgc tcgccgaggc cggcgcggcc gaggtcgacg ccctgtatgc
     3361 cgccgggccg ggccgggagg ccgacacggc aggatcggtg ggccgtctgg ggtcggtggg
     3421 cggtccgggg tcggtcgggg gctcaggttc ggtgggcggc ggcccgga
//
LOCUS       DAORPW010000237         3467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_9001_length_3467_cov_700.548652, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000237 DAORPW010000000
VERSION     DAORPW010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9001_length_3467_cov_700.548652"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..>3467
                     /locus_tag="PLB86_12540"
     CDS             76..>3467
                     /locus_tag="PLB86_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC66017.1"
                     /translation="MTADLAALGARLRLLTDAQLRAVAGPTTFTRGQGYAAQRRVSGL
                     AASSTWVVSAKVRGSGRIYQTQVELYLSRGEVLWTGECTCPMQVDCKHSVAVAIVARQ
                     TFGLAGAATLATPAAYVSDSDLARWAVVPALDEPASWTDQPPPDEYDRYDYDDDGYPD
                     PLAALHHLHPGAPTTPGLPAWERAVRAVVGDTAATTTHFTPVGLLFEVQVTPPNRYSP
                     PGSAHAKLLVRPMVPGKTGWVRTGLTWGNLDYASPYGQIKLNPVHQSALQAVVAIARQ
                     PYGYRPIADRVDLAVLGRGWFAALRECQRLGIKLLTDPKRSGEVTLAEEPARVVVHLT
                     SDGEGGLLVGPTIVEPAEVAATQGDWEAIGSPATGMWVAHGDDLVIAAFEPAVDVPTQ
                     RWLVEGGQRVPAGDVARFFAGHLPALAKRFDVLSPDGSVEVPTVGAPRLLLRLTHEPG
                     LRLSLAWSFTYAVGQDRVEVPVGSSTEPTIRDLAAEERLIDGLDALDKAPGLRTARPT
                     VGWSLQPRSGLDGSPMLRFLNLVLPRLEEHPDVDIEVLGTPIPYAEADEAPTVSLAIT
                     ESSDSTDWFDLDVQVRVGPQDVPIRSLVTALTLGEEHVILPSGTWFRVDRPELTELRR
                     LIDEARAMSDDPKGPLRLSAYQADLWAELEELGVVQEQSERWRSLVAGLAGAAEGAPP
                     EPVPAPEGLAAELRHYQHSGYEWLTFLRRAGLGGILADDMGLGKTMQTLAMILRAVQD
                     REEAADAEGATGAGGERAAYPPFLVVAPTSVLPTWLAEAARFAPSLRVVALGETTKRR
                     RGSIAEAIAGADLVVTTYAVFRIDADAFRECAWSALVLDEAQFVKNHQSQVYQCARLL
                     PARTKLAITGTPMENNLMELWALLSIVAPGLFPRPAAFAEHFRRPIESGTAPERLDTL
                     RRRIRPVMLRRTKDAVASELPPKQEQVIEVALSPKHRRIYDRVLAHERQRILGLLDDA
                     QRNRIAILRSLTRMRRLCLDPVLDDPAHAGIGSAKVDALLELLTPVLAEGHQALVFSQ
                     FTGFLATVRARLDAEGISYAYLDGRTRKREEAIASFRSGERSMFLVSLKAGGFGLTLT
                     EADYVFLLDPWWNPATEAQAIDRTHRIGQDKHVFVYRMVAADT"
BASE COUNT          487 a         1275 c         1204 g          501 t
ORIGIN      
        1 gcctgtggat agcgcggggt gggtgtcggc gggacggcat acagtgctcg ggtccgtatc
       61 acttcgggag ccgtgatgac cgcagacctg gccgccctcg gggcgcgact gcgtctgctc
      121 accgacgcgc agttgcgtgc cgtcgccggt ccgacgacgt tcacccgcgg ccaggggtat
      181 gccgcgcagc gccgggtttc cgggctcgcg gcgagcagca cctgggtggt gagcgcgaag
      241 gttcgcgggt cggggcggat ctatcagacc caggtcgagc tctatctcag ccgcggcgag
      301 gtgctctgga ccggcgaatg cacctgtccc atgcaggtcg actgcaagca ctccgtcgcc
      361 gtcgccatcg tcgctcgcca gaccttcggg ctggccggag cggccaccct cgccaccccc
      421 gcggcatacg tgtccgattc ggatctcgct cgctgggcgg tcgttccggc cctggatgag
      481 ccggcctcgt ggacggatca gccaccgccc gacgaatacg acaggtatga ctacgacgac
      541 gacggctacc ccgacccgct cgcggccctg caccacctcc accccggcgc gccgaccacc
      601 ccgggcctgc cggcctggga gcgggccgtg cgcgcggtcg tgggcgacac ggcagcgaca
      661 acgacccact tcaccccagt tgggttgctg ttcgaggtgc aggtcacccc gcccaaccgc
      721 tacagcccac ccggctccgc ccacgccaag ctgctcgtgc gcccgatggt ccccggcaag
      781 acgggctggg tccgcaccgg gctcacctgg ggcaacctcg actacgccag cccctacggc
      841 cagatcaaac tcaaccccgt ccaccagagc gcgctgcaag cggtcgtcgc catcgctcgc
      901 cagccctatg gctaccgacc gatcgccgac cgggtcgacc tggcggtgct ggggcgcggc
      961 tggttcgccg cgctgcgcga gtgccagcgc ctcggcatca aactcctcac cgaccccaag
     1021 cgcagtggcg aggtcacgct ggccgaggag ccagcgcgcg tcgtcgtgca cctgacgagc
     1081 gacggcgagg gcggtctcct cgtcgggccg acgatcgtcg aacccgcaga agtcgctgcc
     1141 acccaaggcg attgggaagc gatcggctca cctgcgaccg gcatgtgggt ggcacacggc
     1201 gacgacctcg tgatcgccgc cttcgagccg gcggttgacg tgccgaccca gcgctggctc
     1261 gtcgagggcg ggcagcgggt gccggcaggc gatgtggcgc gcttcttcgc cggccacctc
     1321 cccgccctgg ccaagcgctt cgacgtgctc tcccctgacg gcagtgtcga ggtgccgacg
     1381 gtgggcgcgc ctcgcctcct cctgcggctc acccacgaac cagggctgcg gctctcgctc
     1441 gcttggtcct tcacgtatgc cgtggggcag gaccgcgtgg aggtccccgt cgggtcgagc
     1501 accgaaccga cgatccgcga cctcgctgcc gaggagcggc tgatcgacgg cctcgacgcg
     1561 ctcgacaagg cacccggcct gcgcactgcc cggccgacgg tgggctggtc gctgcagccc
     1621 cgctcgggcc tggacggctc gccgatgctg cgcttcctca acctcgtgct cccccgcctc
     1681 gaggagcatc ccgatgtcga catcgaggtg ctcggcacgc cgatccccta tgccgaggcc
     1741 gacgaggccc cgacggtcag cctggcgatc accgagtcga gcgactcgac cgactggttc
     1801 gacctcgacg tgcaggtgcg ggtcggccca caggacgtgc cgatccgcag cctggtcacg
     1861 gccctgacgc tcggcgagga gcacgtcatc ctgcccagcg gcacgtggtt ccgggtcgac
     1921 cgacccgagc tgacggagct gcgccggctc atcgacgaag cccgggcgat gtctgacgac
     1981 cccaagggtc cgctgcggct ctcggcctat caggccgacc tctgggccga gctcgaggag
     2041 ctcggcgtcg tccaggagca gagcgagcgc tggcggtcgc tcgtggccgg gctcgcgggt
     2101 gcggccgaag gcgccccgcc cgagcccgtg cccgcccccg aagggctggc cgccgagcta
     2161 cggcactacc agcacagcgg ctacgagtgg ctgaccttcc tgcgccgggc cgggctcggc
     2221 ggcatcctcg ccgacgacat gggtctgggc aagacgatgc agaccctcgc gatgatcctg
     2281 cgcgcggtgc aggaccgcga ggaggcggcc gacgcagagg gcgcgacggg cgcgggaggt
     2341 gagcgggcgg catacccccc gttcttggtg gttgccccca cctcggtgct gccgacctgg
     2401 ctcgccgagg ccgcgcggtt cgcaccgtcg ctgcgggtgg tcgcgctcgg cgagacgacc
     2461 aagcgccgcc gcggcagcat cgccgaggcc atcgcggggg ccgacctcgt ggtgacgacg
     2521 tatgccgtgt tccgcatcga cgccgacgcc ttccgggaat gcgcgtggtc ggcgctcgtg
     2581 ctcgacgagg cgcagttcgt caagaaccac cagtcgcagg tctatcaatg cgcgcggctg
     2641 ctgcccgccc ggaccaaact cgccatcacc ggcacgccca tggagaacaa cctcatggag
     2701 ctctgggcgt tgctgtcgat cgtcgcgccg gggctgttcc cgcggccggc cgcgtttgcc
     2761 gagcacttcc ggcggccgat cgagagcggc accgcgccgg agcgcctcga cacgctgcgg
     2821 cggcggatcc ggccggtcat gctgcggcgc accaaggacg cggtggccag cgagctgccc
     2881 cccaagcagg agcaggtcat cgaggtcgcg ctctcgccga aacaccgccg gatctatgac
     2941 cgggtgctcg cgcatgagcg gcagcggatc ctcgggctcc tggacgatgc ccagcgcaac
     3001 cggatcgcca tcctgcggtc gctgacccgg atgcggcggc tgtgcctcga cccggtgctc
     3061 gacgacccgg cccacgcagg gatcgggtcg gccaaggtcg atgcgctgct cgagctgctc
     3121 accccggtgc tcgctgaggg gcatcaggcc ctggtgttca gccagttcac cgggttcctc
     3181 gcgacggtcc gagcgcggct ggatgccgag gggatcagct atgcctacct cgacgggcgc
     3241 acccgcaagc gcgaggaggc catcgcgtcg ttccggtcgg gcgagcggtc gatgttcctc
     3301 gtgtcgctca aggccggcgg gttcgggctg accctgaccg aggccgacta tgtcttcctc
     3361 ctcgacccgt ggtggaaccc cgcgaccgag gcccaggcga tcgaccgcac ccaccggatt
     3421 gggcaggaca agcacgtctt cgtctatcgg atggtcgccg ccgacac
//
LOCUS       DAORPW010000238         3451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_9077_length_3451_cov_1089.475559, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000238 DAORPW010000000
VERSION     DAORPW010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9077_length_3451_cov_1089.475559"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLB86_12545"
     CDS             complement(<1..338)
                     /locus_tag="PLB86_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRC66018.1"
                     /translation="MYAASLATAEPLTTTPLTRRPQGDFSAACTQRLPGTTADGLVVP
                     TSRGPRDYANLDHAASTPALTVVAEAVERTLRTYASVHRGNGFASRVTSEWYEQAREE
                     VARFIGARED"
     regulatory      complement(375..492)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            760..3384
                     /locus_tag="PLB86_12550"
     CDS             760..3384
                     /locus_tag="PLB86_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRC66019.1"
                     /translation="MPFPLRAVRGAWLGVSHVAGSAVRKVGHSAAELEPEHRRDGVGF
                     LLIAVAIVVAAREWWGFQGVIGDVVHAVAAGTFGRIAYALPLVLLVFGLRLLRAPDDD
                     ATTNRVVVGTLTLTFAACGIAHLADGIPTPPAGADGMRAAGGIFGFLASSPLAAAVSP
                     VGALVLLLMLGLFGLLVITATPVRMIPQRLREVRDRILGRAPAPTETDDGAATGATKR
                     SRRPVIDLAAKRDGDDAFEQAAEAPGEGAGRKGLRPGQRRPEPEPAIESTDPTAHDAP
                     PVEPATRAGGGAGAGKRKAELIAPPTSTLPLRGEQLSLAGDIAYVLPDNALLAPGAPH
                     KARSAANDRVVESLTQVFEQFEIDASVTGFSRGPTVTRYEVELGLATKVERVTALSKN
                     IAYAVASADVRILSPIPGKSAIGIEIPNTDRETVSLGDVLRSQTARANDHPMVMGVGK
                     DVEGGYVIANLAKMPHLLVAGATGAGKSSFVNSMITSILMRATPEEVRMILVDPKRVE
                     LTAYEGIPHLITPIITNPKKAAEALQWVVREMDQRYDDLASFGFKHIDDFNKAVRTGK
                     VTPLPGSERVITPYPYLLIIVDELADLMMVAPRDVEESIVRITQLARAAGIHLVLATQ
                     RPSVDVVTGLIKANVPSRMAFATSSLADSRVVLDQPGAEKLVGQGDALFLPMGTSKPM
                     RVQGAWVTESEIHGVVKAVTLQLQPQYREDVVVPVAASRQVEDDIGDDLEVLLQAAEL
                     VVTTQFGSTSMLQRKLRVGFAKAGRLMDLLESRGIVGPSEGSKAREVLVKPDDLPTTL
                     ALLRGEDVPAEAWVPEGVPGEVDGDSGDTAYVLDGDAHTKAQAGGRYPSDPLAGSDDV
                     PGWDEDEGGDAWELTGRD"
BASE COUNT          493 a         1201 c         1245 g          512 t
ORIGIN      
        1 tcgtcctcgc gggcgccgat gaagcgggcg acctcctcgc gggcctgctc gtaccactcg
       61 ctggtcacgc ggctggcgaa gccgttgccg cggtggaccg aggcgtaggt gcgcagggtg
      121 cgctcgacgg cctccgcaac cacggtgagg gccggggtcg acgcggcgtg gtcgaggttg
      181 gcgtagtcgc gcgggcctcg ggaggtgggg acgaccaggc cgtcggccgt cgtcccaggc
      241 agccgctgcg tacacgcggc gctgaagtcg ccctgcggac ggcgggtgag cggcgtggtg
      301 gtcagcggct ctgcggtggc gagcgatgcg gcatacatcg gaggtctcct gcgggtccgt
      361 gggcccgggt gacgggtccg cgcttgccgg tcagcgaggt ccgctgacaa gcccggtcgt
      421 cacccggagc accccgccgc agaggagggt tgccgatcag caagccgggg ctgtgcgctg
      481 acactcatga ccttgggtag aagataacgg cacgtgatgc ggcctgtgaa gcctggtccg
      541 ggttctgaga cggaatttcg cggctgtgac gcgtgtggcg catggggttg gcgtggctgg
      601 tggcgtaacc tccggatcat ggcgacacga ccgtctggag cgtccgcacg cccacagacc
      661 accacccgga gcacgtccgc gcgctccgca aacccccggt cgcgcaccgg cacaccccgg
      721 cgcgcccccc ggtcctcgcg gcggcccaag ggcggcggta tgccgttccc gctgcgggcc
      781 gtgcgcggag cctggctcgg ggtgtcgcac gtcgccggca gcgccgtccg caaggttggc
      841 cactccgccg ccgaactcga gccggagcac cgtcgtgacg gtgtcggctt cctgctcatc
      901 gcggtcgcga tcgtcgttgc ggcgcgtgag tggtggggct tccagggggt catcggcgac
      961 gtcgtccatg ctgtcgccgc cgggacgttc ggacggatcg cctacgccct gccgctggtg
     1021 ctgctcgtgt tcggcctgcg cctgcttcgc gctcccgatg acgacgcaac gaccaaccgg
     1081 gtcgtggtcg gaaccctcac cctgaccttc gccgcctgcg ggatcgccca cctggcggac
     1141 ggcataccga cgccgccggc cggtgccgac ggaatgcgcg cggccggagg catcttcggc
     1201 ttcctcgcct ccagcccgct ggccgctgcc gtctcgcccg tcggggcgct cgttctgctg
     1261 ctcatgctcg gccttttcgg gctgctcgtc atcaccgcga ccccggtgcg gatgatcccg
     1321 cagcggttgc gtgaggtgcg tgaccggatc ctcgggcggg ccccggcgcc gaccgaaacc
     1381 gacgacggtg ccgcgacggg ggcgaccaag cgctcgcgcc ggcccgtcat tgacctggct
     1441 gccaagcgcg atggcgatga cgccttcgag caggccgccg aggcgccggg cgagggcgcc
     1501 gggcgcaagg ggctgcggcc cgggcaacga cgtcccgagc ccgagccggc catcgagtcg
     1561 accgatccca ccgcccacga cgcaccgccg gtcgagccgg ccacccgcgc gggtggggga
     1621 gcgggcgcgg gcaagcgcaa ggcggaactc atcgcgccgc cgaccagcac cctgccgctg
     1681 cgcggggagc agttgtcgct cgccggggat atcgcctacg tgttgcccga caacgcgctg
     1741 ttggcccctg gcgcacccca caaggcgcgt tcggcggcca acgaccgggt cgtggagtcg
     1801 ctcacccagg tgttcgagca gttcgagatc gacgcctccg tcaccgggtt cagccgtggg
     1861 ccgacggtca cgcgctacga ggtcgagctg ggtctggcca ccaaggtcga gcgcgtcacc
     1921 gcgttgtcca agaacatcgc gtatgccgtc gcgtcggccg acgtgcggat cctctcgccg
     1981 atcccgggca agtccgcgat cggcatcgag atccccaaca ccgaccgcga gaccgtgtcg
     2041 ctgggcgatg tgctgcgcag ccagaccgcc cgggccaacg accacccgat ggtcatgggg
     2101 gtcggcaagg acgtcgaggg cgggtacgtc atcgccaacc tcgccaagat gccgcacctg
     2161 ctcgtcgccg gggcgaccgg ggcgggcaag agcagcttcg tcaactcgat gatcacctcg
     2221 atcctcatgc gggccacgcc tgaggaggtc cggatgatcc tcgtggaccc caagcgcgtg
     2281 gagttgaccg cctatgaggg gattccgcac ctcatcacgc cgatcatcac caaccccaag
     2341 aaggcggccg aggcgctgca atgggtcgtg cgcgagatgg accagcgcta tgacgacctg
     2401 gccagcttcg ggttcaagca catcgacgac ttcaacaagg cggtgcgcac cggcaaggtg
     2461 accccgctgc ccggcagtga gcgggtcatc acgccctacc cctacctgct gatcatcgtc
     2521 gacgagttgg ccgacctcat gatggtcgcg cctcgggacg tcgaagagtc gatcgtccgg
     2581 atcacccagt tggcgcgggc ggccggcatc cacctggtcc tggctaccca gcgccccagc
     2641 gtcgatgtcg tcacgggcct catcaaggcc aacgtgccct cccggatggc gttcgccacc
     2701 tcgtccctgg ccgatagccg ggtcgtgctc gaccagcccg gcgccgagaa gttggtgggt
     2761 cagggcgacg cactgttcct gccgatgggg acctccaagc cgatgcgtgt ccagggtgcc
     2821 tgggtgaccg agtcggagat ccacggcgtc gtcaaggccg tcaccctgca gttgcagccg
     2881 caatatcgcg aggacgtcgt tgttccggtg gccgccagcc gtcaggtcga ggacgacatc
     2941 ggcgacgacc tcgaggtgct gctgcaggcg gccgagctcg tcgtgacgac gcagttcggg
     3001 tcgacgtcga tgctgcagcg caagttgcgg gtcggcttcg ccaaggccgg tcggctcatg
     3061 gatctgctgg agtcccgcgg catcgtcggg ccgtcggagg ggtccaaggc gcgcgaggtc
     3121 ctcgtcaagc ccgatgacct gcccacgacc ctcgcgctgc tgcgcggtga ggatgtcccg
     3181 gccgaggcct gggtgcccga gggcgtgccg ggcgaggtgg acggcgacag cggcgacacg
     3241 gcatacgtgc tggacggcga cgcccacacc aaggcgcagg cgggcggacg ctatccgagc
     3301 gacccgctgg ccggctccga cgacgtgccc ggctgggacg aggacgaggg cggcgacgcc
     3361 tgggaactca ccggccgcga ctgactctga gcgcggcacg gagtgcgatg ggtggggtga
     3421 tcaccccctt gaacgcactc cgtcgcgcac t
//
LOCUS       DAORPW010000239         3437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_9134_length_3437_cov_567.263749, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000239 DAORPW010000000
VERSION     DAORPW010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9134_length_3437_cov_567.263749"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..683
                     /locus_tag="PLB86_12555"
     CDS             177..683
                     /locus_tag="PLB86_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRC66020.1"
                     /translation="MRRIVTWALSTITTLVLLMSYHTSTSSTASTVNASGVTPGSASK
                     GAAPGSVPNSGGSAGTGNGTTGGSSNGSASSADGTTFAGAAVPTRFGNVQVQITVVDK
                     QITAATVLQIPNRDRKDIQINNRAVPILNSATVKAQSADIDMVSGATVTSVAYIQSLQ
                     SAIDKANL"
     gene            680..1420
                     /locus_tag="PLB86_12560"
     CDS             680..1420
                     /locus_tag="PLB86_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRC66021.1"
                     /translation="MTRRAWVEHHMGMPMSIHLRGDDLARPEVEKAVATAYALLARMD
                     EIFSTWSPDSQVSRLRRGELALSDCDPLVHQAARIGGQAEELTSGAFTTLLPDADGMP
                     RFDPTGLVKGWAVDLAGDELRGLPGVSWCINAGGDVRAGRHQHVPPTGGDAMAWRIGI
                     ENPYDRSQIARVVPLTDGAVATSGTAARGAHLYDPHTRQAVGRPGSTSVVGPDLLWSD
                     IWATALFVGGATTRAAFAEHPAYEVVDL"
     gene            complement(1422..3434)
                     /locus_tag="PLB86_12565"
     CDS             complement(1422..3434)
                     /locus_tag="PLB86_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRC66022.1"
                     /translation="MPNRLAQALSPYLRQHADNPVDWWEWSEAAFAEARHRDVPVLLS
                     VGYAACHWCHVMAHESFEKDDVAAAVNAGFVAVKVDREERPDVDAVYMAATVALTGSG
                     GWPMTVLLTPEGEPFWCGTYLPREQFLGLLDAASSAWADRRGAIQASSAQIMAAVRSQ
                     VAPTAPEPITRHTVADAVTRLGRAADPVNGGFGRAPKFPAAMVLEFLLRHHGRTGSPE
                     ALGLVEEACTAMARGGIYDQLAGGFARYAVDAAWVVPHFEKMLYDNALLARVYAHWWR
                     ATGSPLAARIAVETCEFIVRELGTAQGGFAAALDADTDGVEGLTYVWSRAELVEVLGA
                     EDGDRAADLLTVTAEGTFEHGRSTLQLQGDPDDPSWWAGVRTRLLAARATRPQPARDD
                     KVVTSWNGLAIAALAEVGTLLGRADFVAAAAAAADLILDLHLVDGRLRRVSRDGSVGT
                     AQGCADDYGNLAEGLLTLAQVTGEARWLSAAGTLLDTAVALFGDADNEAPGTFYDTPG
                     IADDTDERLLPTRPHSSADNAEPSGQSSLAVALLGFGALAGSTSHLELADAALAVAGS
                     VAERDPRFAGWTLAGAEARLAGPLQVAIVGTGADAEALRQVALASPSPGLVLAAGQPD
                     APGVPLLAGRPLVDGAAAAYVCRGFVCDRPVTTPADLAAALRPVAG"
BASE COUNT          537 a         1253 c         1185 g          462 t
ORIGIN      
        1 gacgtcgacg cgttgcgtca cctcgtcccc gacctcgccg accacgacgt ctacatctgc
       61 ggcaaccccc agtggatgga tcgcgtcgaa gaggcggcgc tcgccggcgg cgtgccggag
      121 gccaacatcc acgtcgaacg cttcgtctac tgatcctgac cggaagagaa ccgcgcatgc
      181 gacgcatcgt cacctgggca ctgagcacga tcacgaccct cgtgctgctc atgagctacc
      241 acacgtcgac ctcgagcacg gcctccaccg tcaacgcctc cggcgtgacc cctggcagcg
      301 catcgaaggg ggcggctccc ggctccgttc cgaactccgg tgggtcagcg ggcaccggca
      361 acggcacgac gggcgggtca tcgaacggct cggcgtcgag cgccgacggc acgacgttcg
      421 cgggggccgc cgtgcctacc cgcttcggca acgtccaggt gcagatcacc gtcgtcgaca
      481 agcagatcac cgcagcgacg gttctgcaga tccccaaccg ggaccgcaag gacatccaga
      541 tcaacaaccg cgcggtcccg atcctcaaca gcgcgaccgt caaggcccag agcgccgaca
      601 tcgacatggt gtccggggcc acggtcacct ctgtcgccta catccagtcg ctgcagtcgg
      661 cgatcgacaa ggccaatttg tgacccgacg ggcctgggtc gagcaccaca tggggatgcc
      721 gatgagcatc cacctgcgcg gtgacgacct ggcccggccg gaggtcgaga aggccgtggc
      781 gacggcatac gccctccttg cccggatgga cgagatcttc tccacgtggt cgccggactc
      841 ccaggtgagt cggctgcggc gcggcgaact ggcgctgagc gattgcgacc cgctcgtcca
      901 ccaggcggcc cgcatcggtg gtcaggcgga ggaactcacg agcggcgcct tcacgacgtt
      961 gttgcctgac gcggacggta tgccgcgctt cgacccgacc gggctcgtca aggggtgggc
     1021 ggtcgacctg gccggcgacg aactgcgcgg ccttcctggt gtgtcgtggt gcatcaacgc
     1081 aggcggcgac gtccgagccg gtcggcacca gcacgtgcct ccgaccggtg gggacgctat
     1141 ggcgtggcgg atcggcatcg agaaccccta cgaccgcagc cagatcgccc gagtcgtccc
     1201 gctcaccgac ggggccgtcg ccacgtcggg caccgcggcg cgaggtgccc atctgtatga
     1261 cccgcacacc cgtcaggccg tcgggcggcc cgggtcgaca agcgtcgtcg gccccgacct
     1321 gctgtggtcg gacatctggg ccacggccct gttcgtcggc ggcgcgacga cccgagctgc
     1381 gttcgccgag cacccggcat acgaggtcgt cgacctctga gtcaccccgc gacgggacgc
     1441 agcgctgccg ccaggtcggc gggcgtggtc accggacgat cgcacacgaa cccgcgacac
     1501 acgtatgccg ccgccgcgcc gtccacgagt gggcgccccg cgagcagggg cacgccgggg
     1561 gcgtcgggct ggccggcagc cagcaccaac ccgggggagg gactggccag cgccacctgt
     1621 cgcagcgcct cggcgtcggc gccggtcccg acgatcgcca cctgcagcgg tccggccagc
     1681 cgggcctcgg cccctgccag agtccaaccg gcaaagcggg gatcccgctc ggcgaccgac
     1741 ccagcaacgg cgagcgcggc gtcggccagc tccaggtgag acgtcgaccc ggcgagcgcg
     1801 ccgaagccga gcagagccac ggccagggac gattgacccg acggttcggc gttgtcggcg
     1861 gacgagtgcg gccgggttgg caggaggcgc tcgtcagtgt cgtcagcgat gcccggcgtg
     1921 tcgtagaacg tgcccggggc ctcgttgtcc gcgtcgccga acagggcgac ggccgtgtcg
     1981 aggagcgtcc ccgcagccga gagccaccgg gcctccccgg tgacctgggc cagggtcaac
     2041 aggccctcgg cgaggttccc gtagtcgtcg gcgcacccct gtgccgtccc gacggatccg
     2101 tcccgcgaga ctcgccgcag ccgcccatcg acgaggtgca ggtcaaggat caggtctgcc
     2161 gcggctgccg ctgccgcaac gaaatcggcc cgccccagca gggtgccaac ctcggcgagc
     2221 gcggcgatcg ctagcccgtt ccacgaggtg acgaccttgt cgtcacgggc cggttgcggt
     2281 cgggtggctc gggctgccaa cagcctggtg cgcacccccg cccaccacga cgggtcgtcg
     2341 ggatcgccct gcaactgcag cgtcgatcgg ccgtgttcga aggtcccctc ggccgtcacc
     2401 gtgagcaggt ccgcggcccg atccccgtcc tcggcaccca atacctcgac caactcggcc
     2461 ctggaccaga cataggtcag gccttcgacg ccgtcggtgt cggcgtccag cgctgccgcg
     2521 aatccgcctt gggcagtgcc caattcgcgc acgatgaact cacaggtctc gacggcgatc
     2581 cgagccgcga ggggcgaccc cgtggcgcgc caccagtgtg cgtagacccg ggccaacaga
     2641 gcgttgtcgt agagcatctt ctcgaagtgc gggaccaccc aggcggcgtc cacggcatac
     2701 ctggcgaagc cgccggcgag ttggtcgtag atgccgccgc gcgccatggc cgtgcaggcc
     2761 tcctcgacga ggccgagcgc ttcgggcgag ccggtccgcc cgtggtggcg cagcaggaac
     2821 tccagcacca tcgcggcggg gaacttcggc gcgcggccga agcccccgtt gaccgggtcg
     2881 gccgcgcgtc ccagccgggt caccgcgtcc gccacggtat gccgcgtgat cggttccggc
     2941 gcggtcggcg cgacctgcga acgcaccgcc gccatgatct gggcactgct ggcttggatc
     3001 gccccgcgcc gatcagccca ggccgacgac gctgcgtcga ggaggccgag gaactgctct
     3061 cggggcaggt aggtcccgca ccagaacggc tcgccctcgg gggtgagcaa cacggtcatc
     3121 ggccagccac ccgagccggt gagtgccacg gtggcggcca tgtagacggc gtcgacatcc
     3181 gggcgttcct cgcgatcgac cttgacggcg acgaagccgg cgttgaccgc cgcggccacg
     3241 tcgtccttct cgaacgactc gtgagccatg acatggcacc agtggcacgc ggcatacccg
     3301 acggacaaca ggaccgggac gtcgcggtga cgagcctccg cgaaggccgc ctccgaccac
     3361 tcccaccagt ccaccgggtt gtcggcatgt tgacgcaggt aggggctgag ggcctgcgcg
     3421 agtcggttcg gcatgcc
//
LOCUS       DAORPW010000240         3382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_9386_length_3382_cov_655.526300, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000240 DAORPW010000000
VERSION     DAORPW010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9386_length_3382_cov_655.526300"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..831
                     /locus_tag="PLB86_12570"
     CDS             40..831
                     /locus_tag="PLB86_12570"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HRC66023.1"
                     /translation="MTTTVATPTLPPVGIVALAAVAGLQGLLDRFAEGVGAGVDVRYA
                     ASSVTEVDRAIRRLQAIKLKLVAAADRAEVSRGSGHSGTGAWLAAQTHSSGSAAAAQV
                     ALAAALHGAVPGGAGPSEGLSLTSAALSAGRLSPEHAAVIASTSRQLPASLDVAERLR
                     VEAALVEQAQRVDPARLRRTARRALTFAGRADAEVAAHEDAVLRTEESRAFERARLTL
                     HEPRRDDERAFHGAGLRRSDPAQDHPAVGVSASVARESTGRGALG"
     gene            776..1369
                     /locus_tag="PLB86_12575"
     CDS             776..1369
                     /locus_tag="PLB86_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRC66024.1"
                     /translation="MASPRRWRESRPAAARSDDLAIDWAQRYGQAFTELIEHLPTDRL
                     TGKVAATVVVTLDAQRLADLVNSQPQASGVDTGLTLSAGQARRIACNAGLLPAVLGGP
                     SLPLDLGRADRFFTEAQRVALATIYDECAADGCDRPYAWSELHHEDPWHAGGRTDLAQ
                     AVPLCGWHHRRIHDPAYRHSVTTAPTGAKSVTFHLRT"
     gene            1755..2873
                     /gene="infC"
                     /locus_tag="PLB86_12580"
     CDS             1755..2873
                     /gene="infC"
                     /locus_tag="PLB86_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRC66025.1"
                     /translation="MKEHHISEPRINERIRVPEVRLVGPNGEQVGIVRVEDALRLAAE
                     ADLDLVEVAPMARPPVAKLMDFGKYKYEAALKAREARKNQVNTVIKEIKLRPKIDPHD
                     YGTKKGHVERFLKGGDKVKVTIMFRGREQSRPELGFRLLQKLAEDISELGFVESAPKQ
                     DGRNMIMVIGPTKKKAEAKAEARRARESHSPSGQARSAGSAHAAGDASADEHFDLAEN
                     PSDDSLSLEAQVELAEMAEAAEAIEAAEAAEAAEAAAAHAVVPDAAAATADDIAESVD
                     DAVDEIAPEPAEAPAAPAPVKRAARAVPAPAPAAAATPAAAAPATPGSATPKPAVPRP
                     SAPRPAAPRPTATKAAAPRSSTARPTPAPRPGPRPTSG"
     gene            2952..3146
                     /gene="rpmI"
                     /locus_tag="PLB86_12585"
     CDS             2952..3146
                     /gene="rpmI"
                     /locus_tag="PLB86_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRC66026.1"
                     /translation="MPKMKTHSGAKKRFRVTGTGKIMREQAGGRHRLEVKSSKEMRSL
                     AGDLELSPPDAKKAKRLLGR"
     gene            3178..>3382
                     /gene="rplT"
                     /locus_tag="PLB86_12590"
     CDS             3178..>3382
                     /gene="rplT"
                     /locus_tag="PLB86_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRC66027.1"
                     /translation="MARVKRAVNAQKKRRVVLERASGYRGQRSRLYRKAKEQMLHSLG
                     YAYRDRRAKKGDFRRLWIQRINAA"
BASE COUNT          548 a         1209 c         1158 g          467 t
ORIGIN      
        1 tacttgccat aggacgtgtg ttcgactaga atcggaccta tgaccacgac cgtcgccacc
       61 ccgaccctgc cgcccgtcgg catcgtcgcg ttggccgcgg ttgctggtct gcaggggctg
      121 ttggatcggt tcgccgaggg ggtcggggcc ggggtcgacg tcaggtatgc cgcgtccagc
      181 gtcaccgaag tcgaccgcgc catccggcgc ctgcaggcca tcaagctcaa gctggttgcc
      241 gccgccgatc gggccgaggt gtcgcggggc tccggtcact cgggcaccgg cgcctggttg
      301 gcggcccaga cgcattcgtc ggggagcgct gccgctgctc aggtggcgct ggccgcggcc
      361 ctgcatgggg ctgtcccagg cggggccggc cctagcgagg gactgtccct gacgagtgct
      421 gcgttgtcgg ctgggcggct ctcacctgag cacgccgcgg tgatcgcctc gacgagccgt
      481 cagttgcccg cgtcgctcga cgtggcggag cgcctgcggg tggaggcagc tcttgtcgag
      541 caggcacagc gggtcgatcc cgcgcggttg cgccgcacgg ctcggcgagc cttgaccttc
      601 gccggtcggg ccgacgcgga ggttgccgcg catgaggatg cggtcctgcg gaccgaggag
      661 tctcgagctt tcgagcgagc gcggctcacc cttcatgaac ctcgacggga cgatgagcgg
      721 gcatttcacg gtgccggcct tcgccggtca gatcctgcgc aagaccatcc agcagttggc
      781 gtctccgcgt cggtggcgcg agagtcgacc ggccgcggcg cgctcggatg acctcgcgat
      841 cgactgggcc cagcgttacg gccaggcgtt caccgagctc attgagcacc ttccgaccga
      901 ccggctgacc ggcaaggtcg cggcgaccgt ggtcgtcacg ctcgatgccc agcgactggc
      961 cgatctggtc aactcccagc cgcaggccag cggcgtcgac accggcctca ccctctccgc
     1021 tggccaggct cgtcggatcg cgtgcaacgc cggtttgctc cccgcagtgc tgggcggccc
     1081 gtcgcttccg ctggacctgg gccgagctga tcggttcttc accgaagccc aacgcgtcgc
     1141 tctggccacc atctatgacg agtgcgccgc cgatggatgc gaccggcctt acgcctggag
     1201 cgagttgcac cacgaagatc cgtggcatgc aggtggccga accgacctgg cccaggcggt
     1261 cccgctgtgc ggctggcacc atcggcgcat ccacgacccg gcctaccggc acagcgtgac
     1321 cactgccccg accggcgcga agtcagtgac attccacctg aggacctgag gcgaagagcc
     1381 gttgacggtg cgacgcgaac cggcgcggac acggcatacc cgcgccgtcc ccgaatggac
     1441 tggctaggcg agtggccctc acctggtaat ctagggaact gaccagccgc gtccgacgca
     1501 ccccttccag ggagcagagg gcgcggtgtg caagagaagc cccgcttcca cccgcgccga
     1561 tcaccagtcg ccgggtcagg tccggacccg cccggctcgc cgggagcggc ggtgtatgcc
     1621 gtcgcgctgg gtcccgcgcg tgtgcgcggc aggcgactgc ccggcatccg cagcggacgg
     1681 acaagcgaag gctcctcgtg caccgacggt gacgaagggc cttctttcgt ttcctggggt
     1741 cactcgtgac cgacatgaag gagcaccaca tcagcgagcc tcgtatcaac gagcgcatcc
     1801 gggtccccga ggtgcgtctg gtcggaccca atggggagca agttggcatc gtccgcgtcg
     1861 aggacgccct gcgcctggct gccgaggccg atctcgacct cgtcgaagtc gcgcccatgg
     1921 cgcgtccccc ggtggccaag ctcatggact tcggtaagta caagtacgaa gccgccctca
     1981 aggccaggga agcgcgcaag aaccaggtca acacggtcat caaggagatc aagctccgac
     2041 cgaagatcga cccgcatgac tacggcacca agaagggcca cgtcgagcgc ttcctcaagg
     2101 gcggcgacaa ggtcaaggtg acgatcatgt tccgaggtcg cgaacagtcg cgcccggagc
     2161 tgggtttccg gctgctgcag aagctcgccg aggacatttc ggagctcggt ttcgtcgagt
     2221 ccgcgcccaa gcaggacggg cgcaacatga tcatggtcat cggcccgacc aagaagaagg
     2281 ccgaggccaa ggctgaagcc cgtcgggcgc gcgagtcgca ctcgccgtcg ggtcaggctc
     2341 gatcggccgg gtcggcgcac gctgccggcg acgcttcggc ggacgagcac ttcgacctcg
     2401 ccgagaaccc gtcggacgac tcgctgtccc tggaagccca ggtcgagttg gccgagatgg
     2461 ccgaagcggc agaggcgatc gaggctgcgg aagcggccga ggcagccgag gcagcagccg
     2521 cgcacgctgt ggtgccggac gctgcggcgg ccaccgctga tgacatcgcc gagtcggtcg
     2581 acgacgcggt cgacgagatc gctcccgagc cggccgaggc gccggccgcg ccggctccgg
     2641 tcaagcgggc agcccgcgcg gttcccgccc cggcgcctgc cgccgcggcc accccggcag
     2701 cggcagcccc tgccaccccc ggctctgcaa ctcccaagcc ggccgtcccc cgccccagcg
     2761 ctccgcgccc ggcggctccg cgaccgactg ccaccaaggc ggccgcccca cgatcgagca
     2821 cggcccggcc cacgccggcg ccgcgaccgg ggccccgtcc cacgtcgggc tgacccagcc
     2881 cgcacccaga ccacatctga ccccgtccga ccgacgtcga gcgggacctg acacgaagga
     2941 gatcggcagc catgccgaag atgaagaccc acagcggggc caagaagcgc ttccgcgtga
     3001 ccggcaccgg caagatcatg cgcgagcagg ccggcggccg tcaccgcctc gaggtgaagt
     3061 cgagcaagga gatgcgctcg ctcgccggtg acctcgagct gtcccccccc gacgccaaga
     3121 aggccaagcg gcttctcggc cgctgagccc gacccactca cgtaaaggag aactcacgtg
     3181 gcacgcgtga agcgggcagt caacgcccag aagaagcgcc gggtcgtcct cgagcgcgcc
     3241 agcggctacc gcgggcagcg ctcgcggctc taccgcaagg ccaaggagca gatgctccac
     3301 tcgctcggtt acgcctaccg tgaccgccgg gccaagaagg gtgacttccg tcgcctgtgg
     3361 atccagcgga tcaacgctgc gg
//
LOCUS       DAORPW010000241         3380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_9397_length_3380_cov_706.661955, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000241 DAORPW010000000
VERSION     DAORPW010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9397_length_3380_cov_706.661955"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..507
                     /locus_tag="PLB86_12595"
     CDS             163..507
                     /locus_tag="PLB86_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66028.1"
                     /translation="MSDARVGGASEIEAEFVDSGVSESVTPDSTPAESGTSQPITPEP
                     ITPESPMPSAAADGLFPAPAPAPPHTGDHVVDRVLADLHVALGRDLDEQIEVADTVGE
                     ALHRRLQDLDSE"
     gene            504..1307
                     /locus_tag="PLB86_12600"
     CDS             504..1307
                     /locus_tag="PLB86_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRC66029.1"
                     /translation="MSPRLDAELVRRGLARSRAQAAQLVKDGSVRLGGVTVLRVSTPV
                     PDDAVLEVVAAADPWVGRAAYKLLGALETFGPRGLTVAGRRALDVGASTGGFTQVLLS
                     QGAAQVTALDVGHGQLVEQVAGDPRVIERSGTNIRHCTAEDLGGPFDLVVADLSFISL
                     RLVLPVLATLIEPSGDLVLLIKPQFEVGRERLGKGGVVRVDADRLTALRGVLDAAADV
                     GLGVRGLVASPIRGTTGNREYLGWFTPRAHDRAEAADLERLVAAMESPS"
     gene            1304..2224
                     /locus_tag="PLB86_12605"
     CDS             1304..2224
                     /locus_tag="PLB86_12605"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRC66030.1"
                     /translation="MTRRVLLVPHPRRPDIGDIARDVATRLAAQGVATTMIATDAAAL
                     HATDIGPTTVVPDAGAANDCELVCVLGGDGTILRGAELSRGTDTPLLGINLGKVGFLA
                     EVEMADLATTVDRIVARDYTVEDRMTLDVTVSHGAGVDSRTWALNEVSIEKAARERML
                     EVTVEIDGRPLSTWGCDGVIIATPTGSTAYAFSAGGPVVWPDVEAILLVPNSAHALFA
                     RPVVVGPRSRLAVEVIRDAEGQGVLWCDGRRHVDLAPGARIDVCRSAVPMRLARLSHA
                     PFTDRLVEKFDLSVAGWRGNGAGRPQPPTD"
     gene            2251..>3380
                     /locus_tag="PLB86_12610"
     CDS             2251..>3380
                     /locus_tag="PLB86_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC66031.1"
                     /translation="MLLEIRIAGLGVIAEATLRLHSGLTVLTGETGAGKTMVVQGLGL
                     LLGSRADPALVRSGRPSLSVEGIVRLPTGHPARERAQDAGGELDEDELVLARTVTAEG
                     RSRAHIGGRSAPVGVLGEIGEQLVAVHGQADQWRLRRPDEHRDVLDGYGGEAVAAALA
                     AYREGYAALTATLTERDTLRGLDRDRTREVDSLTAALEHVARVDPQPGEDEALRAEDT
                     RLAHAEGLRDAAGAAHALLLGDDGYAADAGEQGAATDLLARARAQLGPVARHDPRLSA
                     LDERLAEAGYLVADIGADLAAYLADLDADPARLAWVQQRRADLAGLTRRYGESIDDVL
                     AWSGEAASRLALLQSADERLAALDARVAALTAEVTAAGERLSA"
BASE COUNT          456 a         1239 c         1216 g          469 t
ORIGIN      
        1 ggcgcctgtc gccgaggctg tgtccgccga ggtggctccc tctacggcgg tcgctcctcc
       61 cgcgcccgcg cccgcgcccg ttccggcacc tgccgccgaa tcggcagcgg cagcggctcc
      121 ggccgcagtc gtggaccagg ccgccgaccc cgtcgtgggc gagtgagcga cgcacgagtg
      181 gggggagcgt ccgagatcga ggctgagttc gttgactcag gggtgtccga gtcggtcacc
      241 cctgactcga cgcctgccga gtcaggcacc tcccagccga taacccctga gccgatcacc
      301 cctgagtcac ccatgcccag cgcggcggca gacgggctct tcccggctcc ggcccccgcg
      361 cctccacaca cgggcgacca cgtcgtcgac cgggtgctgg ccgacttgca cgtggccctg
      421 ggccgggacc tggacgagca gatcgaggtc gccgacaccg tcggagaggc actgcaccgc
      481 cgattgcagg acctcgactc ggagtgagcc cgcgcctgga cgccgagctg gttcgacgcg
      541 gcctagcgag gtcgcgcgcc caggcggcgc agctcgtgaa ggacggcagc gtccggctcg
      601 ggggagtgac cgttctgcgg gtgtcgacgc ccgtcccgga cgacgcggtc ctcgaggtcg
      661 tggcggcggc ggacccttgg gtggggcggg cggcatacaa actgctgggc gccctcgaga
      721 ccttcgggcc gcggggactc acggtggcgg gtcggcgcgc cctggacgtc ggtgccagca
      781 cggggggctt cacccaggtc ctgctcagcc agggcgctgc ccaggtgacc gccctggacg
      841 tcggtcacgg gcaactggtc gaacaggtgg ccggcgaccc gcgcgtcatc gagcgctccg
      901 gcaccaacat ccggcactgc accgccgagg acttgggtgg ccccttcgac ctcgtcgtcg
      961 ccgacctctc gttcatctcc ttgcgcctcg tcctgccagt cctggccaca ctcatcgagc
     1021 cgagcggcga tctggtgttg ctgatcaagc cgcagttcga agtcggtcga gagcggctcg
     1081 gcaagggcgg ggtggttcgc gtcgacgctg accggctgac cgcccttcgt ggtgtgctcg
     1141 acgctgccgc agacgtgggg ctcggtgtgc gtgggctggt cgccagcccg atccggggca
     1201 ccacgggcaa ccgggagtat ctaggctggt tcacacctcg cgcgcacgac cgcgccgagg
     1261 ccgcggacct ggaaaggctg gtcgccgcca tggagtcacc ctcgtgaccc gacgcgtgct
     1321 gctcgtccct catccccgac gccccgacat cggcgacatc gcccgtgacg tcgcgacccg
     1381 attggccgcg cagggggtgg ccacgacgat gatcgccaca gatgcggcag cgttgcacgc
     1441 cacggacatc ggaccgacca cggtggtgcc cgacgccggg gccgccaacg actgtgagct
     1501 cgtgtgcgtc ctgggcggtg acgggacgat ccttcggggc gccgaactct cccggggcac
     1561 cgacacgcca ctgctgggga tcaacctcgg caaggtgggc ttcctcgccg aggtcgagat
     1621 ggccgacctg gcgacgacgg tcgatcggat cgtggctcgc gactacaccg tggaagaccg
     1681 gatgaccctc gatgtcaccg tgagccatgg cgcaggcgtc gactcccgca cctgggcgct
     1741 caacgaggtg agcatcgaga aggccgcccg ggagcgcatg ctcgaggtca ccgtcgagat
     1801 cgacggtcga ccgttgtcca cctggggatg cgacggggtg atcatcgcga cgccgacggg
     1861 ctcgacggca tacgccttct cggccggggg accggtcgtc tggccagacg tcgaagcgat
     1921 cctgctcgtg cccaactcgg cccacgcgct gttcgctcgg ccggtcgtcg tcgggcctcg
     1981 atcccggctg gccgtcgagg tcatccgcga tgccgagggc caaggtgtcc tctggtgcga
     2041 cgggcgacgc cacgtggacc tcgccccggg tgcgcggatc gacgtctgcc gctcggccgt
     2101 gccgatgcgt ctggcccggc tgtcgcacgc gccgttcacc gaccggctcg tcgagaagtt
     2161 cgacctgtcg gtggctggct ggcgcgggaa cggcgcgggc cgtccacagc cacccaccga
     2221 ctgacggcgc gggcccttag tcttgggccc gtgctcctgg agatccgcat cgccgggctt
     2281 ggcgtcatcg ccgaggccac cctcaggctg cactccggcc tgacggtcct caccggagag
     2341 acgggcgccg gcaagacgat ggtcgtccag gggctcgggc ttctgctggg ctcccgcgcc
     2401 gatcctgccc tcgtgcgctc cggtcggccg agcctgtcgg tcgagggcat cgtgcgactg
     2461 cccaccggac acccggcccg ggagcgggcg caggacgccg gcggtgagct cgacgaagac
     2521 gagttggtgc tggcccgcac cgtcactgcc gagggccgat cgcgcgcgca catcggcggc
     2581 cggtcggcgc ccgtcggggt gctcggcgag atcggcgagc agctcgtcgc cgtccacggg
     2641 caggccgatc agtggcgact gcgccgaccc gacgagcacc gggacgtcct cgacggttac
     2701 ggcggcgaag cggtcgccgc cgcgctcgcg gcataccgag aggggtatgc cgccctcacc
     2761 gcgaccctga ccgagcgcga caccctgcgc ggcctggacc gcgaccgcac ccgggaggtc
     2821 gactccctca ccgccgctct cgaacacgtc gcccgggtcg accctcaacc gggagaagac
     2881 gaggcgctgc gcgccgagga cacccgactg gcccacgccg agggcctgcg cgatgccgca
     2941 ggcgctgccc acgcgctcct gctgggcgac gacgggtatg ccgccgacgc gggggagcaa
     3001 ggcgccgcaa cggacctgct cgccagggcc cgagcgcagc tcggaccggt cgccaggcac
     3061 gaccctcgac tgtccgctct cgatgagcgc ctcgccgaag ccggctacct cgtggccgac
     3121 atcggggccg acctcgcggc atacctcgcc gacctcgacg ccgacccggc ccggttggcg
     3181 tgggtgcagc agcggcgagc cgatctagcc ggcctcaccc gccgctacgg cgaaagcatc
     3241 gacgacgtgc tggcctggtc cggcgaggcg gcgtcgcgcc tggccctgct gcagtctgcc
     3301 gacgaacgcc tcgccgccct cgatgcgcgg gtggcggcgt tgaccgccga ggtgacggcc
     3361 gccggcgagc ggctcagcgc
//
LOCUS       DAORPW010000242         3358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_9471_length_3358_cov_763.058129, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000242 DAORPW010000000
VERSION     DAORPW010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9471_length_3358_cov_763.058129"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..362
                     /locus_tag="PLB86_12615"
     CDS             63..362
                     /locus_tag="PLB86_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66032.1"
                     /translation="MYLSVCPESLDQVAVSLAARVPELPTPAQVVGDLEAIGACLPAF
                     VNHTPAPSSVTGGGRPSGEPWGRSLRDLGAAWVAHSGALGRSAQSYREVDARPLR"
     gene            359..2104
                     /locus_tag="PLB86_12620"
     CDS             359..2104
                     /locus_tag="PLB86_12620"
                     /inference="COORDINATES: protein motif:HMM:NF018018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC66033.1"
                     /translation="MTAPTIGDVRRARPDALGHLATRVGGHAARLRAAASARATSGPG
                     DTWTGPASSAAGGRHTELMVTARTVADGLDRCAAAIRAAGEQVADAQRTLDQAERAAR
                     AGGTHLLDSGALASTGSALPGSPDTAVVVARLAERARADYGAALFTLRARLTAASGSY
                     AVSPPAQSMAAHSSSASWSPWWSQASPTSISPPPDGPIAASAWFAGLSDIERDEVIRS
                     HPEWVGPRDGLPAWARDRANRLVLESAAQRLMAELAARRDEPTDADLPWRDVLRPQLR
                     SRRVIELIAALDGLRAIKEVLATDDGRRRQLLSLDATGGTLTAAVTSGDLDGAGHVAV
                     FVPGFQADVAGDLASYSRGVEAAADLADRLSRAHGDGRPVVGVTWLGYAAPTVLEVVV
                     PGRSVAGWGPATAGSPALGSLLAGLDAAGRAAHPQEPPRLVLWGHSYGSLVTGLALRD
                     ATVPVAAAVVFGSPGMGVDTVAQLRLPPGQLYVEEALDDVVAATSRFAADPGLWREAT
                     QLSVAQQDLPDGSVGRVAHGHEEYLTAGSTSQWNLAAVAAGTTDLVRRRAPCPPWSPL
                     SQQPPPSCERPMRLG"
     gene            2402..2974
                     /gene="nrdR"
                     /locus_tag="PLB86_12625"
     CDS             2402..2974
                     /gene="nrdR"
                     /locus_tag="PLB86_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRC66034.1"
                     /translation="MHCPFCRHGDSRVIDSRTTDDGTSIRRRRLCPECGRRFTTIETA
                     SLSVVKRSGATEPFSRQKVLVGVRKACQGRPVTEDQLARLAQRVEESVRATGSAEVDA
                     HEVGLAVLEPLRELDEVAYLRFASVYRAFESLEDFEAAITLLRAEREATGREPAHLGG
                     GAAYPSGMPRATEDALAGSGGAADSPAPPH"
     gene            3090..>3358
                     /locus_tag="PLB86_12630"
     CDS             3090..>3358
                     /locus_tag="PLB86_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66035.1"
                     /translation="MTETANRSTARKSPRGKGITVERIYTTPGVHPYDEVTWERRDVV
                     QTNWKSGETIFEQRGVEFPDFWSVNAGAIVTTKYFRGAQGTPVRE"
BASE COUNT          476 a         1192 c         1190 g          500 t
ORIGIN      
        1 cgcttcgcct acagtccagg caagggttgg ggtggtcgag ccgatggcgg aaggagccag
       61 cggtgtacct ctcggtgtgc ccggaatccc tcgaccaggt ggcggtgtcc ctcgccgcac
      121 gcgtcccaga acttcccacc ccagcgcagg tggttggcga cctggaggcc atcggcgcct
      181 gcctgccggc cttcgtcaac cacaccccag cgccctcgtc ggtcacaggt ggcggtcgac
      241 cgtcgggcga gccctggggt cggtcgctga gggacctcgg cgccgcctgg gtggctcact
      301 ccggcgcgct gggcaggtcg gcgcagtcct accgtgaggt cgacgcgagg ccgctgcgat
      361 gacggcgccg accatcggcg atgtccgtcg cgccagaccc gacgcgctgg gccacctcgc
      421 gacgcgcgtg ggcgggcatg ccgcgagact tcgtgcggcg gcgtcggccc gggcgacttc
      481 cggccccggt gacacctgga cgggaccggc gagcagcgct gcgggagggc ggcataccga
      541 gctcatggtc accgcgcgca cggtggcgga cgggctcgat cggtgcgccg cggcgattcg
      601 ggccgcgggc gagcaggtgg ccgacgctca acgcaccctc gatcaagccg agcgagccgc
      661 ccgcgccggg ggaacccacc tgctggactc cggcgccctg gcaagcaccg gctcggcgct
      721 ccctgggtcg ccggacactg cagtggttgt cgcgcgcctg gccgagcgcg ccagagcgga
      781 ctacggagcg gccttgttca cgctgcgggc ccggctcacg gccgcttccg gcagctatgc
      841 cgtctcaccc ccggcgcagt ccatggccgc ccactcgtcg tcggcatcgt ggtcgccatg
      901 gtggtcgcag gcgtccccga cctcgatctc cccacctccg gatggcccga tcgcggccag
      961 tgcgtggttc gccgggttga gcgacatcga gcgcgacgag gtgatccgca gccatcccga
     1021 gtgggtgggg cctcgggacg ggctccccgc ctgggctcgc gatcgcgcca accggttggt
     1081 gctggagagc gccgcccaac ggctgatggc cgagttggcc gcgcgccggg acgagccgac
     1141 cgatgccgac ctaccgtggc gggatgtcct gcgtccccag ttgcgatcgc gtcgcgtcat
     1201 cgagctcatc gccgccctcg acgggttgcg ggcgatcaag gaggtcttgg ccaccgacga
     1261 cgggcggcgc cgccaactgc tgagcctgga cgccaccggg ggcaccctca ctgcggcggt
     1321 cacgtccggt gatctcgatg gtgccggtca cgtcgccgtc ttcgtccccg gcttccaggc
     1381 agatgtggcc ggtgacctgg cgagctactc ccgcggggtc gaggccgcgg ccgacctcgc
     1441 cgatcggttg tctcgggccc acggagacgg ccgcccggtc gtcggggtca cctggctcgg
     1501 gtatgccgct ccgaccgttc tcgaggtcgt cgtgcccggg cggtcggtcg ccggttgggg
     1561 cccggccacg gctgggtctc cggctctggg cagcctcctc gccgggcttg atgctgcggg
     1621 ccgggctgcc catccgcagg agccgccgcg actggtcctc tggggccact cctacggctc
     1681 gctcgtcacg ggcctggcgc tgcgcgatgc gaccgtgccg gtggctgccg ccgtggtgtt
     1741 cgggtcgccg gggatgggcg tggacaccgt cgcccaactg cggttgccgc cgggacagct
     1801 ctatgtcgag gaggccctcg atgatgtcgt cgccgccacc agccgattcg ccgccgaccc
     1861 cggtttgtgg cgcgaagcca cccaactgag cgtcgcgcaa caggatctgc ccgacggctc
     1921 cgtcggacgg gtcgcccacg gccacgagga gtacctcacc gcgggctcga ccagccagtg
     1981 gaacctcgcg gcagtggcgg caggcacgac cgacctggtc cgtcgtcgag cgccctgtcc
     2041 gccgtggtcg ccgctctcac agcaaccacc accctcatgc gagcggccga tgcggttggg
     2101 ttgacgccgc tcagcagagg ttgatccgtg acacgccggt cccgacacgc cgatcggtgc
     2161 caaacctcgc cagcgcttga caggagtagg gccgacaggt cacagttacc cctacatcta
     2221 gtagttacag ccgtgtgatt catccacatg tagtccacag gctggggcga gttacccaca
     2281 cactgtccac acggcatacc tgcctcgtcc acgaggatcc ccaggtttgt ccacaggagc
     2341 ggtgtgtgta ggcaagcgcc tgaggcgcac ggtcagcacg agtcgagggg gaggtctgca
     2401 catgcactgt ccgttctgtc gccatggcga ctctcgcgtc atcgactcgc gcacgaccga
     2461 cgacggcacg tcgatcaggc ggcgccggct gtgccccgag tgcgggcgac gcttcaccac
     2521 gatcgagacc gccagcctgt cggtggtcaa gcggtcggga gcgaccgagc cgttcagccg
     2581 ccagaaggtc ctcgtcggcg tccgcaaggc ctgccagggc cgtccggtca ccgaagacca
     2641 gttggcccgg ctcgcgcagc gggtcgagga atcggtccgg gccaccggca gcgccgaggt
     2701 cgatgcccac gaggtcggcc tggccgtgct cgagccattg cgcgagctcg acgaggtcgc
     2761 ttacctgcgt ttcgcctccg tctatcgggc cttcgagagc ctcgaagact tcgaggcggc
     2821 gatcaccctg ctgcgcgccg agcgcgaggc aaccggacgc gagccggccc acctcggcgg
     2881 cggggcggca tacccctcag gtatgccgcg cgccaccgaa gacgccctgg cgggctctgg
     2941 tggggccgcg gattcaccag caccaccgca ctgaacgacc cggcgccggc gcctgatcga
     3001 ggaatcgaga gggctgtcgg tggtcggtgc tgcacttgac gtacgacgac gagacagacg
     3061 catcagcaag gtcagggagg accccagtca tgacggaaac tgcgaaccgg tcgaccgccc
     3121 gcaagagccc ccggggcaag ggcatcaccg tggagcgcat ctacaccacc cccggtgtgc
     3181 acccctacga cgaggtgacc tgggagcggc gcgacgtcgt ccagaccaac tggaagtcgg
     3241 gcgagacgat cttcgagcag cgcggggtcg agttccccga cttctggtcg gtcaacgccg
     3301 gtgcgatcgt cacgacgaag tacttccgtg gtgcgcaggg gacgccggtg cgcgaatg
//
LOCUS       DAORPW010000243         3282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_9829_length_3282_cov_615.942051, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000243 DAORPW010000000
VERSION     DAORPW010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9829_length_3282_cov_615.942051"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..459)
                     /locus_tag="PLB86_12635"
     CDS             complement(22..459)
                     /locus_tag="PLB86_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC66036.1"
                     /translation="MANDDAARRAELVLLRKVRDRIDRDYAQPLDVDALARGAHLSAG
                     HLSRAFKAAYGESPYSYLMTRRIERAMTLLRRGDLSVTDVCFAVGYGSLGTFSTRFTE
                     LVGMPPSAFRDLDGEALRGIPPCVTQRAGKPVRNREAGAHPLI"
     gene            596..982
                     /locus_tag="PLB86_12640"
     CDS             596..982
                     /locus_tag="PLB86_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC66037.1"
                     /translation="MAIARFPVVVLDCPDPKALAEFYGALLGWEVQPGDGDSGGDSDD
                     GTWWQIRADYGDSMAFQAVEGYRAPQWPGQEHPQQMHIDVVVDDLDVAEAAVLELGAT
                     KHAHQPGTTFRVFLDPAGHPFCLCSS"
     gene            complement(1003..1683)
                     /locus_tag="PLB86_12645"
     CDS             complement(1003..1683)
                     /locus_tag="PLB86_12645"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HRC66038.1"
                     /translation="MFWTVLGEVLTPGLGAALSPMLIVAAIVLGSAPSGPRKVFTFST
                     GVFVVMAVIGMLVVLGADKAEVATVPSRSEGGHILGLIVGILLCGMAWLTYKNRPTNS
                     EDSSSVKRLNSLDALPHPRIFVMGMVLGLVNVKNIPLEISVGTNVVQTGAGVPLSLIG
                     MLVFSAMACSTLVTPGVIVLAAPEKSTRILGHARKVLIANNTAIMIVLYLMLGTNLIS
                     KSLGPLFA"
     gene            complement(1780..2151)
                     /locus_tag="PLB86_12650"
     CDS             complement(1780..2151)
                     /locus_tag="PLB86_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC66039.1"
                     /translation="MSDFAPDPFNPACPSRRLLDRIGDRWTVLIVVALDPGPQRFGAL
                     ASRVGGISQKMLTQTLRSLERDGFVTRTAYPEIPPRVEYALTPLGRSLLGPLRALEEW
                     AVTNLPAVASHQQGYAAAAAD"
     gene            2267..2917
                     /locus_tag="PLB86_12655"
     CDS             2267..2917
                     /locus_tag="PLB86_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRC66040.1"
                     /translation="MARITVLGGSGYAGGAIVAEAAKRGHEVTSVSRTAVAAPVAGVE
                     YAVGSALDPAVLDAVTAGRDVVVVALSPRGDMAGKLEGVVEDLIGRLAGGATRLGYVG
                     GASSLLVSEGGPRLWDVSQEHIPADVKPEIETGLRAFELVKASPATLDWFYVSPPADF
                     GSWFNTESKGTYVLGGDVLQRDADGKSTISAADLALAILDEIETPTHHRGRFTAIA"
BASE COUNT          526 a         1019 c         1203 g          534 t
ORIGIN      
        1 gtcaggttca cgcccctcac gctagatgag ggggtgggcg cctgcttctc gattcctgac
       61 cggtttgccg gcgcgctggg tcacacacgg gggtatgccg cgcagcgcct cgccgtcgag
      121 gtcgcggaag gcgctgggcg gcataccgac aagctcggtg aagcgcgtcg agaaggtgcc
      181 gagcgagccg tagccaacgg cgaaacacac gtcggtcacc gacaggtcgc cgcgccgcag
      241 gagggtcatc gcccgctcga tccggcgggt catgaggtag ctgtagggcg actcgccgta
      301 ggccgccttg aaggcgcggc tgaggtgacc cgccgacagg tgcgcgccgc gggcgagagc
      361 atcgacgtcg aggggctgcg cgtagtcgcg gtcgatccgg tcgcggacct tgcgcaggag
      421 cacaagctcg gcgcgccggg cggcgtcatc gttggccacc gggcaagcgt ctcaggaaac
      481 gtggtttgct cggcggaggg gggccgtgag ggggagtgat ggggtgtgaa aggacctgta
      541 cttcgggtac ggaattgaag acaaagtcgt gcggccggcg ttggtagtgt cgggcatggc
      601 tatcgctcgt ttccctgtgg tcgtgttgga ctgccccgac ccgaaggccc tggccgagtt
      661 ctatggggcg ctgctgggat gggaggtcca gcccggtgat ggcgacagtg gcggcgacag
      721 cgacgacggc acctggtggc agatccgcgc ggactacggc gactcgatgg cgttccaggc
      781 cgtcgagggt tatcgggcgc cgcagtggcc cggccaggag cacccccagc agatgcacat
      841 cgacgtggtc gtcgacgatc tcgatgttgc cgaggccgcc gtgctcgagt tgggcgcgac
      901 caagcatgcg catcagcccg gcacgacctt ccgggtcttc ctcgaccccg ccggccaccc
      961 gttctgcctc tgctccagct gagcccgttc actccgccgg cgtcaagcga acagtgggcc
     1021 gagggacttg ctgatcaggt tcgtgcccaa catgaggtag agcacgatca tgatggccgt
     1081 gttgttcgcg atgaggacct tgcgcgcgtg gccgaggatg cgagtggatt tctctggtgc
     1141 cgcgaggacg atcacgccgg gggtcacgag ggtgctgcag gccatcgcgg agaagaccag
     1201 catgccgatc aggctgagcg gcacgccggc gcccgtttga acgacgttcg tgccgaccga
     1261 gatctccagc ggaatgttct tgacgttgac caggccgagc accatgccca tgacgaagat
     1321 tcgcggatgc ggcaaggcgt ccaaggagtt cagtcgcttg accgaggacg agtcttcact
     1381 gttggtaggc cggttcttgt aggtcaacca cgccatgccg cacaagagga tgcccacgat
     1441 cagtccgaga atgtgcccgc cctccgaccg cgacggcacc gtggcgacct cagccttgtc
     1501 tgctcccaac accacgagca tcccgatcac cgccatgacc acgaagacgc ccgtcgagaa
     1561 ggtgaacacc ttgcgcgggc ccgatggcgc gctcccgagg acgatcgcag ccacaatcag
     1621 catcgggctc aaggcggcac ccaggccagg ggtgagcacc tcgccaagga cggtccagaa
     1681 cacgggtgtc gctccctcga actatgacca acccttcgat ggtaaccacg tcagcaagac
     1741 cccgagaagg gctcggacgc agcggcgagg gccccgcgct cagtcggcgg cagcggcggc
     1801 atacccctgc tggtggctgg cgacagcggg cagattggtg acggcccact cctccagcgc
     1861 gcgcaacggc ccgagcaggg agcgcccgag gggggtgagc gcatactcca cacgcggcgg
     1921 gatctccggg tatgccgtgc gcgtcacgaa gccatcgcgc tctagcgagc gcagggtctg
     1981 ggtgagcatc ttctgcgaga tgcccccgac gcgggaggcg agcgctccaa accgttgcgg
     2041 gccggggtcg agcgcgacga cgatcaggac ggtccaccgg tcgccgatcc ggtcgagcag
     2101 ccgccgcgac gggcaggccg ggttgaacgg atctggtgcg aaatcgctca cgatgccggc
     2161 tcttctctgc ggggctcggg tcacgcaccc caaagtgcgt actcccggga tggctttaca
     2221 tgacttatgg taacccagta acccacagag agtattggag agagtcatgg cacggatcac
     2281 ggttctgggc ggcagcggct acgcgggcgg ggcgatcgtc gccgaagccg ccaagcgcgg
     2341 acacgaggtc acgtcggtca gccgcaccgc cgtcgcggcg cccgtcgcgg gcgtggagta
     2401 tgccgtgggg tcggcgctcg acccggccgt ccttgacgcg gtgacggcgg ggcgggacgt
     2461 ggtcgtcgtt gccttgtcgc cgcgcgggga catggcgggc aaactcgagg gcgtcgtgga
     2521 agacctcatc ggtcggctgg ctgggggtgc gacgcggctg gggtatgtcg gcggcgcgtc
     2581 gtcgctgctg gtgtcggagg gcgggccgag gctgtgggat gtctcccagg agcacatccc
     2641 ggccgacgtg aagcctgaaa tcgagacagg gctgcgggcc ttcgagttgg tgaaggcgtc
     2701 cccggcgact ctggactggt tctatgtcag cccgcctgct gacttcgggt cgtggttcaa
     2761 cacggagtcg aaggggacgt atgtgttggg cggcgacgtg ctgcagcgcg acgccgacgg
     2821 caagtcgacc atctcggctg ccgacctcgc gctggcgatc ctcgacgaga tcgagactcc
     2881 gacgcatcac cgtgggcgct tcaccgcgat tgcctgattc ggtctggcgg cccggctacg
     2941 cggccgggcc gcctgagtcg aaccaagggt ctgcctcggg gttggggtcg cagcggtccc
     3001 ggtgatcagt tgctcggcgg tcgttcccgt ttcccgaggc tgcccagaga tctgcccagg
     3061 ggtcggcctc ggggttggcg acgtactgct cgtgttggcc ggttgacgag cgaccgctgg
     3121 gttgccacaa gtcggcccag gggtctgcct cgggattggc gatgtatgag tcccggccat
     3181 ccaacgccgg gcggtcgtca tggcgtcgcg agccgcccca ggggtcggcc tcgggggcag
     3241 agttgtccca ctcatggtgg tcgggtgatg gacggttcag gg
//
LOCUS       DAORPW010000244         3272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_9881_length_3272_cov_528.127759, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000244 DAORPW010000000
VERSION     DAORPW010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9881_length_3272_cov_528.127759"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLB86_12660"
     CDS             <1..336
                     /locus_tag="PLB86_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRC66041.1"
                     /translation="NIGKVPVQCGDRSGFIVNALLFPYLNDAVKMLEAHYATADDIDV
                     AMKAGCGYPMGPFELLDVVGLDVSLAIQRELYMEFREPGFAPAPLLEHLVTAGYLGRK
                     SKRGFRTYA"
     gene            461..772
                     /locus_tag="PLB86_12665"
     CDS             461..772
                     /locus_tag="PLB86_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66042.1"
                     /translation="MPRPNRRRDESTPLDLERATGGVPRREGYAGQEWFVRPVRGARA
                     DSAGAPRAYRCPGCQQEVGGQAHVVVWPADGLGGVEQRRHWHTTCWANRHTRPPLGSY
                     R"
     gene            complement(832..1515)
                     /locus_tag="PLB86_12670"
     CDS             complement(832..1515)
                     /locus_tag="PLB86_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRC66043.1"
                     /translation="MFATPSADKQRRRALRSAPSGPLRDYLQTPFPDPRTEVGDLQLL
                     AVDLETTGLHPKNDRLLSIGFVPIDGLSIDLSGARRFVVQAGVEVGQSAAIHGLTDDT
                     VAAGTPIDEALPVVLEALRGRVLLAHYTDIEERFLSAACERVYGTRLPVARIDTLELH
                     RRLIVGWGQEPMRLALRLWAARERYGLPDYRAHECLIDAIACAELYLAQVAEMRSHGD
                     SVTLARLVS"
     gene            complement(1524..>3272)
                     /locus_tag="PLB86_12675"
     CDS             complement(1524..>3272)
                     /locus_tag="PLB86_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HRC66044.1"
                     /translation="RGTRIISRGEDNHHLYVVRSGAVELHDMHGGFMDRGETGTSFGS
                     YTLVQGNPANFEVVALEDTLCLVMDAETFHDLAAAYPIIGNYFDIQRTSRMRGAVASL
                     HVTSSGGAILKTKVTDIITRPAVSVEASESIRAAASVMSERRVSCLLVMDEGKLVGIV
                     TDRDLRNRVVAAGVDTSLPVSQVMSADPATGSADALAFQILMEMTSRNIHHLPILGCD
                     GGPEGVVTTTDLMRLEQTNPIYLVGDITQATDVAAVAEVSKRLAPVVELLVTQDASAE
                     DIGRIVTAVGDAVERRLLGLAEEHLGPPPVPYCWVSLGSRARQEQALAADQDNALILD
                     NDVQPEHAAYFEALATFVCDALVECGYPRCPGDVMATNPRWRVPLSQWRAEFTTWLTE
                     PVPDAILRASIFFDMRPVYGDPRLFQKLRKHFLKQAPAARLFLAHLTKRATENEPPLG
                     FFRGFVLEKHGANRATLDIKRGGVGAIVEIARVLALSVGSRAVNTQTRIAAAVDSGAL
                     SAERGQDLHDAFEFISYVRLRHQAAQVRADRETDNFVAPDDLSSFEKRHLREAFAIVR
                     SAQSSLSHRYPSTYIS"
BASE COUNT          497 a         1102 c         1187 g          486 t
ORIGIN      
        1 aacatcggca aggtgccggt gcagtgtggt gaccgctcgg gcttcatcgt caacgcgctg
       61 ctcttcccct acctcaacga cgccgtcaag atgctcgagg cgcactacgc cacggccgac
      121 gacatcgacg tcgccatgaa ggccggctgc ggttacccca tggggccgtt cgagttgctc
      181 gacgtcgtcg ggctcgacgt gtcgctggcc atccagcgcg agctctacat ggagttccgt
      241 gagcctggct tcgcgccggc tccgctgctg gagcacctgg tcacggccgg ctacctcggg
      301 cgcaagtcca agcgcggttt ccgcacctac gcctgaggcc cgtcggaggg ccaggccggg
      361 cgggaagccg tcgttgaagc agcggcaggg tgcgtgatca cgcaccctgc cgctgcttca
      421 tgcgctgcct cctacccgcc gacctgggag gatggcgtgc atgcctcgcc ccaaccgccg
      481 ccgcgacgag tccacaccgt tggaccttga gcgggcgacc gggggagtcc ccagacgcga
      541 ggggtatgcc ggtcaggagt ggttcgtccg ccccgtccgg ggagcgcggg cggactccgc
      601 aggcgcgccg cgggcctatc gttgcccggg ctgccagcaa gaggtgggcg gccaggccca
      661 tgtcgtcgtc tggccggccg acgggttggg tggcgtcgag cagcgccggc actggcacac
      721 gacatgctgg gccaaccggc ataccaggcc gccgttgggg tcctatcgct gaggtgaccc
      781 gccctcgccc tcgcgggccg tcagggcgtc aggcacccgg accgcgatcg ctcaggacac
      841 cagccgcgcg agggtcaccg agtcgccgtg ggagcgcatc tcggccacct gggcgaggta
      901 gagctccgcg cacgcgatgg catcgatgag gcactcgtgg gcgcgatagt cgggcaggcc
      961 gtagcgctcg cgggcggccc acaggcgcag ggctaggcgc atcggctcct gcccccaccc
     1021 gacgatcaac cggcgatgca actcgagcgt gtcgatccgg gcgaccggca accgagtgcc
     1081 gtagacccgc tcgcaggcag ccgacaggaa gcgttcctcg atgtcggtgt agtgcgcgag
     1141 caggacccgg ccgcgcaggg cctccagcac gaccggcagc gcctcgtcga tcggtgtccc
     1201 ggcggccacg gtgtcgtcgg tcagcccgtg gatcgccgcg ctctgaccga cctcgacccc
     1261 ggcctgcacg acgaaccgcc gggctccgga caggtcgatc gacaacccgt cgatgggcac
     1321 gaagccgatc gagagcaggc ggtcgttctt ggggtgcagg ccggtcgtct cgaggtcgac
     1381 ggccaacaac tgcaggtcac cgacctcggt gcgggggtcg gggaaggggg tctgcaggta
     1441 gtcccgcaac ggccccgacg gcgcgctgcg cagcgcgcga cggcgttgct tgtcggccga
     1501 aggagtggcg aacacgcggg cccctaggag atgtacgtcg aggggtagcg gtgcgacagc
     1561 gacgactggg ccgagcgcac gatggcgaac gcctcacgca ggtggcgctt ctcgaatgag
     1621 ctcaggtcgt cgggggccac gaagttgtcg gtctcgcggt cggcccgcac ctgcgcggcc
     1681 tggtggcgca gccgcacata ggagatgaac tcgaaggcgt cgtgcaggtc ctggccgcgc
     1741 tcggcactca acgcaccgga gtcgacggcg gccgcgatcc gggtctgggt gttgaccgcc
     1801 cgcgacccca ccgacagggc cagcacgcgg gcgatctcga cgatcgcgcc cacaccgccc
     1861 cgcttgatgt cgagagtggc ccggttggcg ccgtgcttct ccaacacgaa gccgcggaag
     1921 aagcccaacg ggggctcgtt ctcggtggcc cgcttggtga ggtgggccag gaagagtcgg
     1981 gccgccggag cctgcttgag gaagtgcttg cgcaacttct ggaacagcct cgggtcgcca
     2041 tagacgggcc gcatgtcgaa gaagatgctc gctcggagga tggcgtcggg caccggctcg
     2101 gtcagccagg tggtgaactc ggctcgccac tgcgagagcg ggacccgcca gcgcgggttg
     2161 gtcgccatga cgtctcccgg gcaccggggg tagccgcact cgacgagggc atcgcacacg
     2221 aaggtcgcca gcgcctcgaa gtatgccgcg tgctcgggtt ggacgtcgtt gtccaggatg
     2281 agggcgttgt cctggtcggc cgccagggcc tgctcttggc gggcccgcga gcccagcgag
     2341 acccagcagt aggggaccgg cggtgggccg aggtgttcct cggccaggcc gagcagccgc
     2401 cgttcgaccg cgtcgccgac ggcggtgacg atccgcccga tgtcctcggc cgacgcgtcc
     2461 tgggtgacga gcaactcgac caccggcgcc agccgcttgc tgacctcggc caccgccgcg
     2521 acatcggtcg cctgggtgat gtcacccacg agatagatgg ggttggtctg ttccaggcgc
     2581 atgaggtcgg tggtcgtgac gacgccctcg gggccgccgt cgcagccgag gatcggcaga
     2641 tggtggatgt tgcggctggt catctccatg aggatctgga acgccaacgc gtcggccgat
     2701 ccggtggcgg ggtccgccga catcacctgg ctcaccggca ggctggtgtc gacgccggcc
     2761 gcgacgacgc ggttgcgcag gtcgcggtcc gtgacgatgc cgacgagctt gccctcgtcc
     2821 atgacgagca ggcaggagac ccgccgctca ctcatgacgg atgccgccgc gcggatcgac
     2881 tccgacgcct ccacggacac cgccggccgg gtgatgatgt cggtgacctt cgtcttgagg
     2941 atcgcgccac ctgagctcgt cacgtgcaac gaggcgacgg cgccgcgcat ccggctggtc
     3001 cgctggatgt cgaagtagtt gccgatgatc gggtaggcgg cggcgaggtc gtggaaggtc
     3061 tcggcatcca tcaccaggca cagggtgtcc tcgagggcga ccacctcgaa gttggccgga
     3121 ttcccctgca cgagcgtgta ggagccgaag gacgtgcccg tctcgccgcg gtccatgaac
     3181 ccgccatgca tgtcatgcaa ctccacggcc ccggagcgca cgacatagag gtgatggttg
     3241 tcctcgccgc gggagatgat ccgcgtcccg cg
//
LOCUS       DAORPW010000245         3260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_9943_length_3260_cov_436.332293, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000245 DAORPW010000000
VERSION     DAORPW010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9943_length_3260_cov_436.332293"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..667
                     /locus_tag="PLB86_12680"
     CDS             332..667
                     /locus_tag="PLB86_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HRC66045.1"
                     /translation="MRARARAYCQFAADHPGNYRVMFTAVPIAPRAGAIEDLPGARIV
                     LELAACIGRCAQSGATPPFDALTSATLLWTTLHGIVSLRVSKPAFPWAPTDELVDRAL
                     LALCAIPLP"
     gene            955..2127
                     /locus_tag="PLB86_12685"
     CDS             955..2127
                     /locus_tag="PLB86_12685"
                     /inference="COORDINATES: protein motif:HMM:NF017812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HRC66046.1"
                     /translation="MRRRLSERDIKAIAEGSAILGTGGGGDPYLGSVYAVRAVREFGE
                     PELVSIDELPDDTLAVAAVMIGSPVPMMEKLSLGDEVERAFEGLQSVLGRPINAMLTS
                     EIGGVNTVISIAIAAERGLPVVDADAMGRAFPEVQLVTQTIYGLRVCPLSMADEHGNV
                     VIINAVDNFWAERLARPTVIEFGAISPSLGFPMTKADIDLASIPGSVSMARDIGEALL
                     FGDPREGGVLQAVLDRTGGVEVFRGKVSSVDRHVTGGWAIGDVFIEGSDAFAGDTMRV
                     TFQNENLMAYLGDEPVVCVPDLITIVEQDTCRAITTERLRFGSRVSVLAMPCDDKWLT
                     PAGLALAGPRHFGYDVDYAPYAGRRPSPGLAPNLGSGPETTAAPMTAPATSDATRS"
     gene            2130..>3260
                     /locus_tag="PLB86_12690"
     CDS             2130..>3260
                     /locus_tag="PLB86_12690"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRC66047.1"
                     /translation="MQIGIDIGGTNTDAVLMADGKVLATYKSPTTASVGEGVHKAISQ
                     VLASAGVSTEVVTRVVLGTTHFTNAVVQRQGLDQVCAIRLGAPASRAFPPFSVWPDDL
                     ATAAHGSSAILKGGVNFDGSPITALDETELRTAADRAVDAGFTSVALTSIFAPLSRDN
                     EQTARDIMAAQQSSLTFTLSSDIGNLGVLERENATILNACLTRMAVDVVASIEAALAS
                     LGIACPFFFSQNDGTLMSAEFAARFPVRTFASGPTNSIRGAGFLAGVQDALVVDIGGT
                     TTDVGALRRGYPRESANTTELAGLRTNFRMPDLVSIGIGGGSLVAEIGGGAANSNAGI
                     SNAGIRVTVGPRSVGRLLTEQALVFGGATLTATDIAVAAGLLD"
BASE COUNT          508 a         1205 c         1051 g          496 t
ORIGIN      
        1 actccacgaa gaagtaggcg aagttcagca gcgccacggt caggacgacc cggcgtagcc
       61 cggcagcgtt ggccgtcagg tccgcctgcg gcgtggtgtc gtcgtccact cgcccaacgc
      121 tagttgccgc cggccagggg caggttcgca gcgtgctggc cacatcggga ggtagccaac
      181 actccgccaa cccggtcgac gccgacccag catctacctc cacttcgccg agctgcacca
      241 actggtcgat gccgtcctcg aaacccgctt cggtgagctg cgcgagctca tcaacgacgc
      301 agccgatgga tccgccacgc ccgcagacga attgcgcgcc cgcgcccgcg cctactgcca
      361 gttcgccgca gaccaccccg gcaactaccg ggtgatgttc accgccgttc ccatcgcgcc
      421 acgagcaggc gccatcgagg acctccccgg cgcacgcatc gtcctcgagc tcgccgcctg
      481 catcggccga tgcgcccaga gcggcgccac gcccccgttc gacgcactca cgagcgccac
      541 actcctgtgg accacgctcc acggcatcgt cagcctgcgc gtcagcaaac ccgccttccc
      601 ctgggccccc accgacgagc tcgtcgaccg agcgctcctc gccctctgcg ccatccccct
      661 gccgtgacca gcgcggctgc cgcacggcga agcgacgggc cagaagacat ccgttgagtg
      721 agtgcccggc ggagtgcgat tgatggggtg atcaccccat caatcgcact ccgtcgcgca
      781 cacggacgag aaggcaggtg agtgccgggc cagttcacaa ccgcgaactg gcccaatctg
      841 ccggtgtgca gggcgccacc tcaaccgcct gcgggtgggc aacgggacca agggcggcgc
      901 gcccggccgt ggatatgttc gggccacgaa tcagcgccga tgaagggctc gctcatgcga
      961 cgaaggctca gcgaacggga catcaaggcg atcgccgagg ggtcggcgat cctcggcacc
     1021 ggcggcgggg gtgatcccta cttgggatcg gtgtatgccg tccgcgccgt ccgcgagttc
     1081 ggcgagccgg agctggtctc gatcgacgaa ctgcccgacg acaccctcgc ggtcgcggcg
     1141 gtcatgatcg gcagccccgt cccgatgatg gagaagctca gcctcggtga cgaggtggaa
     1201 cgagccttcg aggggttgca atcggtcctc ggccgcccga tcaacgcgat gctgacctcg
     1261 gagatcggcg gggtgaacac cgtcatctcg atcgccatcg ctgccgagcg cggcctgccg
     1321 gtcgtcgacg ccgacgccat ggggcgggcc tttcccgagg tacagctggt cacccaaacc
     1381 atctacggcc tccgggtctg cccgctgtcg atggccgacg aacacggcaa cgtcgtcatc
     1441 atcaacgcgg tcgacaactt ctgggccgag cggctggccc gtccgacggt catcgagttc
     1501 ggggcgatct cgccgtcact gggcttcccg atgaccaagg ccgacatcga cctggcctcg
     1561 atcccgggat cggtgtcgat ggcccgcgac atcggggagg cgttgctctt cggcgaccct
     1621 cgtgaaggcg gggtgttgca ggcggtgctc gaccggaccg gcggcgtcga ggtcttccgc
     1681 ggaaaggtca gctcggtcga ccggcacgtg accggcggct gggccatcgg cgatgtgttc
     1741 atcgaggggt ccgatgcctt tgcgggcgac acgatgcggg tgaccttcca gaacgagaac
     1801 ctcatggcct acctcggcga cgagcccgtg gtgtgcgtgc ccgacctcat caccatcgtc
     1861 gagcaggaca cctgccgcgc gatcaccacc gagcggctgc gcttcgggtc gcgggtcagc
     1921 gtgctcgcga tgccgtgcga cgacaagtgg ctgacgcccg cgggtctcgc gctcgccggc
     1981 ccgcgccact tcggttacga cgtcgactat gccccgtatg ccggtcgccg accgtcgccg
     2041 ggactcgcac cgaatctcgg gtcgggtcca gagaccactg ccgcgccgat gaccgccccc
     2101 gccacctccg acgcaacgag gagctgaccg tgcagatcgg cattgacatc ggcggcacca
     2161 acaccgacgc cgtgctcatg gcggacggga aggtgctggc cacctacaag tcgccgacca
     2221 ccgcctcggt gggtgagggt gtgcacaagg cgatcagcca ggtcctcgcc tccgccgggg
     2281 tgagcaccga agtcgtcacc cgcgtcgtcc tcggcaccac ccatttcacc aacgccgtgg
     2341 tccagcgtca ggggctcgat caggtctgtg cgatccggct tggggcgccg gcctcgcggg
     2401 ccttcccgcc cttctcggtc tggcccgacg acctggccac ggcggcccac gggtcctccg
     2461 ccatcctcaa gggtggcgtc aacttcgacg gctcgcccat cacggcgcta gatgagacgg
     2521 agctgcggac ggcggccgat cgggccgtcg atgccgggtt cacgtcggtg gcgctcacct
     2581 cgatcttcgc cccgctcagc cgcgacaacg agcagacggc gcgcgacatc atggccgccc
     2641 agcagtcctc gctgacgttc acgttgtcca gcgacatcgg caacctcggg gtccttgagc
     2701 gcgagaacgc caccatcctc aacgcctgcc tcacccgaat ggccgtcgat gtggtcgcgt
     2761 ccatcgaggc cgccctagcg tcgctcggca tcgcctgccc cttcttcttc agccagaacg
     2821 acgggacgct catgtcggcg gagttcgccg cgcgcttccc ggtgcggacc ttcgcctccg
     2881 gcccgaccaa cagcatcaga ggtgccggct tcctcgccgg cgtccaggac gcactcgtcg
     2941 tggacatcgg cggcacgacg accgacgtgg gcgcgctgcg ccgcggctat ccccgcgagt
     3001 cggccaacac caccgagttg gccgggctgc gcaccaactt ccggatgcct gacctggtct
     3061 cgatcggcat cggcggcggc tccctcgtcg ctgagatcgg cggcggcgcc gccaactcca
     3121 acgccggcat ctccaacgcc ggcatccgcg tcaccgtcgg accgcgatcc gtcgggcggc
     3181 tgctgaccga gcaggccctg gtctttggcg gcgccaccct caccgccacc gacatcgccg
     3241 tcgccgcagg ccttttggac
//
LOCUS       DAORPW010000246         3235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_10066_length_3235_cov_926.675157, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000246 DAORPW010000000
VERSION     DAORPW010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10066_length_3235_cov_926.675157"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..462)
                     /locus_tag="PLB86_12695"
     CDS             complement(10..462)
                     /locus_tag="PLB86_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66048.1"
                     /translation="MTAALAAWTRRRWLAALVAAATTTVLMGVPTALIPTPIFGREVP
                     PTAWAWPVLLATSVLSGLLLATYVREPGQAEPATTASRSGTIGGLLSFLAVGCPVCNK
                     LALLALGYSGALQWFAPVQPFLAAVGVVLLAGALRQRLRGQLACPVPA"
     gene            complement(571..1614)
                     /gene="mgrA"
                     /locus_tag="PLB86_12700"
     CDS             complement(571..1614)
                     /gene="mgrA"
                     /locus_tag="PLB86_12700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HRC66049.1"
                     /translation="MTYRTDDYRAASDRYDRMPMRRCGRSGLVLPAVSLGLWHNFGDD
                     VPLERQQAILRRAFDLGVTHFDLANNYGPPYGSAERNFGTIFRRDLRPYRDELILSTK
                     AGYDMWPGPYGQGGGSRKYLLASLDQSLQRMGVDYVDIFYSHRFDPDTPLEETMGALD
                     SAVRQGKALYAGISSYSGARTREAVQILRELGTPLLIHQPSYSMLNRWIETDLLDVLG
                     DTGVGSIAFSPLAQGMLTDRYLTGIPADSRAAQGKSLDPALLSEAALTHIRALNEIAS
                     ERGQSLAQMALAWALRDPRVTSVLVGASSVAQLEANVAAVDNLEFSAAQLQAIDAHAV
                     ESGINLWKQSSGE"
     regulatory      1698..1815
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            1853..3166
                     /locus_tag="PLB86_12705"
     CDS             1853..3166
                     /locus_tag="PLB86_12705"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HRC66050.1"
                     /translation="MSDTWAFETKQVHAGQSVDSTTGARALPIYQTTSYVFNNTEHAA
                     NLFALKEFGNIYTRIMNPTQDAVEQRIAALEGGLAALLVASGQAAETLAILNIAEAGD
                     HVVASPSLYGGTFNLLKYTLPKFGIEVSFVENTSDLDSWRAAVRPNTKLFFAETISNP
                     KVEILDIEGVAGVAHEAGVPLIVDNTIATPYVIRPLEWGADIVVHSATKYLGGHGTSI
                     GGVIVDGGTFDFGKDPEKFPNYNTPEESYHGLVFARDLGVGGGLGANLAFILKARVQL
                     LRDLGPAISPFNAFLINQGIETLSLRVERHLENTRKVAEFLKAHPQVESVRWASLPDS
                     PEYAKAQKYAPKGSGAVLAFEIVGGLDAGKTFVEALTLHSHVANIGDVRSLVIHPAST
                     THSQGGDADRLAAGVTPGLVRLAVGIEHIDDILADLTQGFAASAG"
BASE COUNT          551 a         1142 c         1069 g          473 t
ORIGIN      
        1 ctgccgcgct caggcgggca cggggcaggc gagctgtccg cgcaaacgct gacgcaacgc
       61 gcccgcgagc aggacgacgc cgaccgccgc gaggaacggc tggaccggcg cgaaccactg
      121 cagcgcaccg ctgtaaccga gcgcgagaag cgcgagtttg ttgcacaccg gacagccgac
      181 ggcgaggaag gacaacaggc cgccgatggt gcccgaccgg cttgccgtcg tcgccggctc
      241 ggcctggccg ggctcacgga cataggtggc caacagcaga ccgctgagca cgctcgtggc
      301 cagcaggacc ggccaggccc aggccgtcgg tggcacctcg cggccgaaga tgggcgtcgg
      361 gatcagcgcg gtcggcacgc ccatgaggac ggtcgtcgtg gcggctgcca ccagggcagc
      421 gagccagcgt cgtcgggtcc aggcggccag cgcggcggtc atccggcgat aataccggca
      481 ggggtatgcc gccctccgcc gcccacacgc gcggcggccc gcctaggcag gcgagctgag
      541 accggtctca accactgcgc ggggcccggc tcactcaccc gatgattgct tccacaggtt
      601 gatcccggac tcgacggcat gtgcgtcaat ggcttgcaat tgcgccgcgc tgaactccag
      661 gttgtccacc gcggcgacgt tggcttccaa ttgcgcgacg ctggaggcgc cgaccaacac
      721 cgaggtgacc cgcgggtcac gcaacgccca ggccaacgcc atctgagcca ggctctgacc
      781 acgctcggag gcgatctcgt tcaacgcccg gatgtgggtg agcgcggcct cggacaacag
      841 cgccgggtcg agcgacttcc cttgggcagc acgcgaatcc gccggtatgc cggtcaggta
      901 tcggtcggtg agcatgccct gggccaaggg cgagaaggcg atgctcccga cgccggtgtc
      961 gccgagcacg tcgagcaggt cggtctcgat ccaccggttg agcatcgagt aggacggctg
     1021 gtggatcagc aggggagtgc ccaactcgcg caggatctgg accgcctctc gggtgcgcgc
     1081 accggagtag ctggagatac cggcatacaa ggccttgccc tgacgcaccg cgctgtccaa
     1141 cgcgcccatg gtctcctcca gcggagtgtc ggggtcgaag cggtggctgt agaagatgtc
     1201 gacgtagtcg acccccatcc gctgcagcga ttgatccagc gaggccagga ggtacttgcg
     1261 ggagccgccg ccctgcccgt agggccccgg ccacatgtcg tagccggctt tggtcgacag
     1321 gatcagctcg tcccggtagg gccgcagatc gcgccggaag atcgtgccga agttgcgctc
     1381 ggcgctgccg tagggcggcc cgtagttgtt ggccaggtcg aagtgcgtga cacccaggtc
     1441 gaaggctcgg cgcaggatcg cctgctggcg ctccagcggg acgtcgtcac caaagttgtg
     1501 ccacaagccc agcgagaccg ccggcaggac gaggccgctg cggccacagc ggcgcatcgg
     1561 catccggtcg tagcgatcgc tggcggctcg gtagtcatcg gtgcggtagg tcatgcggac
     1621 atgctcgcag gtccgggcgg tccacatggt gagccgtccc tgtcccagaa tgcggcccct
     1681 gtttaccctt ccaactaggt catgagtgtc agcgacaagc cccggctcgc tgaccggcaa
     1741 cccttccatc cgcgatgggg tgccccgggt gaagaccagg ctggcgccca accgggccgt
     1801 cggcaagcgc ggggtcgcct ggcgactccc tggcacgaaa gagcattcag cgatgagcga
     1861 cacctgggca tttgaaacca agcaggtcca cgccggacag tccgtcgaca gcacgaccgg
     1921 ggcgcgggcc ctgccgatct accagacgac gtcctacgtc ttcaacaaca ccgagcacgc
     1981 cgcaaacctg ttcgcgctca aggaattcgg caacatctac acgcgcatca tgaacccgac
     2041 ccaggacgcg gtcgagcagc ggatcgccgc cctcgaaggt ggcctcgccg cactgctcgt
     2101 cgcatctggt caggccgctg agaccctggc gatcctcaac atcgccgagg ccggcgacca
     2161 cgtcgtcgcc tctccgtcgc tctatggcgg cacgttcaac ctgctcaagt acaccctccc
     2221 gaagttcggc atcgaggtgt ccttcgtcga gaacaccagc gacctcgact cctggcgcgc
     2281 agcggtccgg cctaacacga aactgttctt cgccgagacg atctccaacc ccaaggtcga
     2341 gatcctcgac atcgagggcg tcgcgggggt tgctcatgag gccggcgtgc cgctcatcgt
     2401 cgacaacacc attgccacgc cctacgtcat ccgcccgctg gagtggggcg ccgacatcgt
     2461 cgtgcactcg gcgacgaagt acctcggtgg tcacggcacg tcgatcggtg gcgtcatcgt
     2521 cgacggcggc accttcgact tcggcaagga cccggagaag ttccccaact acaacacgcc
     2581 cgaggagagc taccacgggc tggtcttcgc ccgcgacctc ggcgtcggtg gggggctggg
     2641 cgccaacctc gccttcatcc tcaaggcccg cgtgcaactg ctgcgcgacc tcggcccggc
     2701 catctcgccg ttcaacgcct tcctcatcaa ccagggcatc gagactctct cgctgcgcgt
     2761 cgagcgccac ctggagaaca cccggaaggt cgccgagttc ctcaaggctc acccgcaggt
     2821 cgagtcggtg cgctgggcgt ccttgccgga cagcccggaa tacgccaagg cccagaagta
     2881 cgcccccaag ggctccggcg cggtgctcgc cttcgagatc gtcggcggcc tggacgcggg
     2941 caagacgttc gtcgaggccc tcaccctgca ctcgcacgtg gccaacatcg gcgacgtgcg
     3001 ctcgctggtc atccacccgg cctccacgac ccactcccag ggtggtgacg ccgaccggct
     3061 cgccgcaggc gtgacccccg gcctggtccg cctggccgtg ggcatcgagc acatcgacga
     3121 catcctcgcc gacctcacgc agggcttcgc ggcctccgcg ggctgacagc ggggcgcgaa
     3181 cctcgcgact gcgggagggc ggcataccga ccgggtatgc cgccctcccg cacgt
//
LOCUS       DAORPW010000247         3227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_10111_length_3227_cov_652.489281, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000247 DAORPW010000000
VERSION     DAORPW010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10111_length_3227_cov_652.489281"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PLB86_12710"
     CDS             complement(<1..749)
                     /locus_tag="PLB86_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HRC66051.1"
                     /translation="MILIDIPIWAAHGTVFAHLVSDTSFEELHEFAARVGMPRGAFDG
                     DHYDVPERRYAACLAAGATRVTGVELVRRVNASGLRLRKRKGEKGIHRWLGTPIGEGV
                     TADIDLVASSMLTPDALTGAAATIVMDRNQSILVVHSVRRGSWDCPGGRREPGETVTD
                     CAARELAEETGLQVPTDGFIPCGYERITVADPGHWAANRPLVQVFRAELNIARPTVVP
                     GDDVDEAHWVSHDEFRDRCRERFWWPLAAFVL"
     gene            899..1186
                     /locus_tag="PLB86_12715"
     CDS             899..1186
                     /locus_tag="PLB86_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HRC66052.1"
                     /translation="MAFTVDTDRIHTASADIGRIAGDIESSVAEMTGRLSALSSSWTG
                     TAATEWQGVLSEWRGLQTRLREDLVTIGQLTARAGTSYQQTEDSVRGLFVH"
     assembly_gap    1204..1303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1304..2026
                     /locus_tag="PLB86_12720"
     CDS             <1304..2026
                     /locus_tag="PLB86_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC66053.1"
                     /translation="ELRRARLTSPLVWYWLLKFAVVAPALVFGYAPRWRGREHLPRSG
                     PFVLAANHTSVIETAIVPFAVNRRVTFVAKSKYYRGQGAKGRLLGWFLRAIGQVPIDP
                     ASASSAQPALDAAREILLGGGVWGIFPEGTRSPDGRMYRGRTGGMRVALPLGVPVIPV
                     AVIGARGSGPWWTWGRNGSRRIHVTYLPPFDLSPWAGREDDPQAWREATEALMATIRE
                     VTGQEYADRYPTPEELHARDNR"
     gene            complement(2066..2740)
                     /locus_tag="PLB86_12725"
     CDS             complement(2066..2740)
                     /locus_tag="PLB86_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66054.1"
                     /translation="MPRAWSELAPLPASDGDWLLPYLRGFGTVGGVVGSQWPRYAALP
                     PTEGQGTDGSLDPTLAAAVREVLARHTDGPGEFVAALWEGWGGLTRGVSAVRLGPGHG
                     CAGTTQQTQIGPALPIEVVNAPRFAPPSRAYLLCAASLEDLGPIPVVSDSNWWTGQQS
                     PSLFWPRSRQWCLGVEIDVVVTLVGGSDALIDELLALPGAVEVDSVERLRREWFGEGF
                     PLAQEY"
     gene            complement(2742..>3227)
                     /locus_tag="PLB86_12730"
     CDS             complement(2742..>3227)
                     /locus_tag="PLB86_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66055.1"
                     /translation="GAAYRALLEQTPMSPDSVLGVVGAAARAWGDPRHSRLTFPTAAV
                     LMIASLVLSFAFFGGWSVISRQGSFDVEWRVVQAAAAGYAALGLFLSRRGHRVVGSVT
                     TGMAVLAFLCQMSLVVPGWSTTDVPIRAAAMWFMSLACWSAWWTAYGVHDPRRRPQAA
                     G"
BASE COUNT          501 a         1150 c         1068 g          408 t
ORIGIN      
        1 agcacgaagg cggcgagcgg ccaccagaag cgctcacggc accggtcccg gaattcgtca
       61 tgcgacaccc agtgggcctc atcgacgtcg tctccgggca cgacagtcgg ccgggcgatg
      121 ttgagctcag cccgaaacac ctgcacgagt gggcgattcg cagcccaatg ccccgggtcg
      181 gccaccgtga tccgctcgta gccgcagggg atgaaaccgt cggtcggcac ctgcaggccg
      241 gtctcctcag ccaattccct tgccgcgcag tcggtcaccg tttcacctgg ctcgcggcgg
      301 ccacccgggc agtcccacga cccgcgccgg accgagtgca ccaccaggat gctctggttg
      361 cggtccatga cgatggtcgc tgcggcgccc gtcagggcat cgggtgtgag catcgacgag
      421 gcgaccagat cgatgtcggc cgtcactccc tcaccgatcg gagtcccgag ccaacggtga
      481 atacccttct cgcccttacg tttacgcaac cgaaggccac tggcattgac ccgccggaca
      541 agctcgacgc ccgtgacgcg agtggccccg gcagccaggc acgcggcata ccggcgctcg
      601 ggaacgtcgt agtggtcccc gtcgaaggcc ccccgaggca tgccgacgcg cgccgcgaac
      661 tcgtgcaact cctcgaacga ggtgtcgctg accaggtgcg cgaacaccgt gccgtgggcc
      721 gcccagatgg ggatgtcgat gaggatcatg tcgctcacac gctagcggcc cgcaccctgt
      781 ggataacttc tgcggcactc ggcgcgatcg gcctaccgtc gagggaagcc ggacatcggt
      841 ccgggtccca tccgcgacgc accgtcgggg gcgcgcggca cgcgatcaag gagcagccat
      901 ggcattcacc gtcgacaccg accggatcca caccgcctcg gccgacatcg gcaggatcgc
      961 cggcgacatc gagtcgtcgg tcgcggagat gaccggccgc ctgtccgccc tctcgagcag
     1021 ctggaccggc accgccgcga ccgagtggca gggggtgctc agcgagtggc ggggtctgca
     1081 gacccggctg cgcgaagacc tcgtcaccat cggccagctc accgcgcggg ccggcacgag
     1141 ctaccagcag accgaggatt cggtccgcgg gctgttcgtc cactgacgcc tccgctgcgg
     1201 gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngagctca ggcgggctcg
     1321 gctaacatcg ccgctcgtgt ggtactggct cctgaagttc gccgtcgtcg cgccggcgct
     1381 ggtgttcggt tacgccccgc gttggcgcgg gcgtgagcac ctgccgcgct ccgggccgtt
     1441 cgtgctggcc gccaaccaca cgtcggtcat cgagacggcc atcgtgccgt tcgccgtcaa
     1501 ccgtcgggtg acgttcgtcg ccaagagcaa gtactaccgc ggccagggcg ccaagggccg
     1561 actgctcggg tggttcctgc gggccatcgg ccaggtgccc atcgacccgg cgtcggcgtc
     1621 gtccgcgcag ccggcgctcg atgcggcgcg ggagatcctg ctcggcggcg gcgtctgggg
     1681 gatcttcccg gagggaacgc gttcgcccga cggccggatg taccgcggcc gcaccggtgg
     1741 gatgcgggtg gccctgcccc tcggcgtgcc cgtcatcccg gtcgcggtca tcggtgcgcg
     1801 cggcagtggt ccgtggtgga cctggggccg caatggcagc cggcggatcc acgtcaccta
     1861 cctgccgccg ttcgacctgt cgccttgggc cggacgcgag gacgacccgc aggcgtggcg
     1921 cgaggcgacc gaggcgctca tggccacgat ccgcgaggtg accggccagg agtatgccga
     1981 tcggtacccg acgcccgaag agctccacgc gcgcgacaac cgctgacgac cgagcgcgct
     2041 gggtcggtga gtcgtcctgc gcgagctagt actcctgcgc gagcgggaac ccctcgccaa
     2101 accactcgcg ccgcagccgc tccaccgagt cgacctcgac cgccccgggc agggccagca
     2161 gctcatcgat cagcgcgtcg ctgcccccga ccagggtgac gacgacatcg atctcgacgc
     2221 cgagacacca ctgccgcgag cgcggccaga acagactcgg tgactgctga ccggtccacc
     2281 agttgctgtc ggacaccacc ggtatcgggc cgaggtcctc gagggacgcc gcacagagca
     2341 ggtaggcccg gctcggaggg gcgaaccgag gtgcgttgac cacctcgatc ggaagcgcag
     2401 gtccgatctg ggtctgctgg gtcgtgccgg cgcagccgtg accaggcccc agcctgaccg
     2461 cggagacccc gcgcgtgagg ccgccccagc cctcccacag ggctgcgaca aactcccccg
     2521 gcccgtccgt atgccgcgcc agcacctcgc ggacggctgc cgccaacgtc gggtccaacg
     2581 agccgtcagt gccctggccc tcggtgggcg gcagggcggc ataccggggc cactgtgagc
     2641 cgaccacccc gccgacggtg ccgaagcccc gaaggtaggg cagcagccag tcaccgtcgg
     2701 aggctggcag cggcgccaac tcgctccacg cgcggggcac gtcagcccgc cgcttgcggg
     2761 cgccggcgcg ggtcgtgcac gccgtatgcc gtccaccacg ccgaccagca cgccagcgac
     2821 atgaaccaca tcgccgccgc ccggatcggc acatcggtcg tgctccaccc cggcacaacg
     2881 agggacatct ggcacaggaa cgccagcacg gccatccctg tggtcactga accaaccacg
     2941 cgatgaccgc gacgggagag gaacaggccg agggcggcat acccggcggc ggcggcctgg
     3001 accacccgcc attcgacgtc gaacgatccc tgccgcgaga tgacactcca cccaccgaag
     3061 aaggcgaacg acaagaccag cgacgcgatc atcaggacgg cggccgtggg gaaggtgagg
     3121 cggctgtggc gcggatcccc ccacgcgcgg gcggccgcgc ccacgactcc gaggacggag
     3181 tccgggctca tcggcgtctg ctcaagcagc gcccggtatg ccgcccc
//
LOCUS       DAORPW010000248         3207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_10213_length_3207_cov_799.761104, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000248 DAORPW010000000
VERSION     DAORPW010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10213_length_3207_cov_799.761104"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1178
                     /gene="arsB"
                     /locus_tag="PLB86_12735"
     CDS             102..1178
                     /gene="arsB"
                     /locus_tag="PLB86_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRC66056.1"
                     /translation="MTTAPTAAEEPAVRLSRVDRLLPVWIGLAMVAGLLLGRLVPGLD
                     QALDAVRIGQTSLPIAIGLLLMMYPVLAKVRYDEIGLVTADRRMIAASLLLNWVVGPL
                     VMFALAWWLLGDHPAYRTGVILVGLARCIAMVLLWNDLACGDREMAAVLVALNAVFQV
                     LMYAVLGYFYLQVLPGWLGLEQAALDVSMWGIASIVLVFLGIPLVAGWLTRWWGERAR
                     GRAWYEGVFLPRIGPIALYGLLFTVVVLFALQGHTITSEPLAVVRIALPLLVYFAVMW
                     VLGYAVGRLLGLTYARTTTMAFTAASNDFELAIAVAIGVFGVASGQALAGVVGPLIEV
                     PVLVGLVYVALWLRGRVRWPESSG"
     gene            1294..1971
                     /locus_tag="PLB86_12740"
     CDS             1294..1971
                     /locus_tag="PLB86_12740"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRC66057.1"
                     /translation="MTARPLAHLVHPSWGDALGPLEPTVTALGAFLREEVAAGRGYLP
                     AGEHVLRAFTQPLDAVRVLVVGQDPYPTPGHPVGLSFSVAPEVRPLPPSLVNIFAELQ
                     TDLGVPRPSTGDLTPWADRGVLLLNRVLTVGPGKPGSHRGRGWEDVTAAAIAALVARG
                     GPLVAILWGRDARSLAPALGDVARIESAHPSPLSARSGFFGSRPFSRANDLLVGQGAE
                     PIDWSLP"
     gene            2017..3204
                     /locus_tag="PLB86_12745"
     CDS             2017..3204
                     /locus_tag="PLB86_12745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRC66058.1"
                     /translation="MSEVFLYDAVRTPFGRYAGALAGIRPDDLGALVIRETVRRAPSL
                     EPERIDEIFFGCANGAGEDNRNVGRMSGLLAGVPTSVPATTLNRLCGSSLDAAMVASR
                     QIALGEADVVVVGGVESMSRAPWVLPKTERPYPAGSMELASTTLGWRLVNPAMPKEWT
                     VSLGEATEQLREKYAVTREAMDEFAARSHFLADAAWSSGFFADQVVAVPGVGLDRDES
                     IRRETTVERLAGLKPSFRSAETGTVTAGNASPLNDGASAALLGSAKADALTGLSPVAR
                     IAGRGAAANDPQFFGYAPVEAANQALARAGITWDDVDAVELNEAFAAQALACVAAWGV
                     DPEIVNQHGGAIAIGHPLGASGTRILGTLARSLQVSGGKWGVAAICIGVGQGLAVVIE
                     NVS"
BASE COUNT          384 a         1119 c         1155 g          549 t
ORIGIN      
        1 cggcctgctc ggcgagctcg gcgtcgcgcc ggtggagttg tctcgatgac cgacgggttc
       61 ccggcgatcg cagacgtcca cgaagggctc gctgaccagt catgacgacg gccccgaccg
      121 cggccgagga accggctgtt cggctctcgc gggtcgaccg gctgttgcct gtgtggatcg
      181 gcctggccat ggtcgccggg ctgctgctcg gtcggctcgt ccccgggctg gaccaagccc
      241 tcgacgcggt ccggatcggc cagacctccc tgccgatcgc catcggcctg ctgctgatga
      301 tgtaccccgt cctggccaag gtccgctacg acgagatcgg cctggtcacc gccgaccgcc
      361 ggatgatcgc ggcatcgctg ctgctcaact gggtggtcgg tcccctggtg atgttcgccc
      421 tggcctggtg gctgctcggt gaccacccgg cataccggac gggggtgatc ctcgtcgggt
      481 tggcgcggtg catcgccatg gtgctgttgt ggaacgacct cgcgtgcgga gaccgtgaga
      541 tggcggccgt gctcgtggcc ctcaacgcgg tgttccaggt gctcatgtat gccgtgctcg
      601 gctacttcta tctgcaggtc ctgccggggt ggctggggtt ggagcaggcg gcgctcgacg
      661 tgtcgatgtg gggcatcgcc tcgatcgtcc tcgtcttcct cggcatcccg ctcgtcgccg
      721 gatggctgac ccggtggtgg ggcgagcgag cccgcgggcg ggcctggtac gagggcgtgt
      781 tcctgccccg gatcggcccg atcgcgctct acgggctgct gttcaccgtc gtcgtgctgt
      841 tcgcgttgca gggtcacacg atcacctccg agccgctcgc ggtggtccga atcgccctgc
      901 cgctgctcgt ctacttcgcg gtcatgtggg tgctcgggta tgccgtgggg cggcttctgg
      961 gactgaccta cgcccgcacg acgacgatgg ctttcaccgc cgccagcaac gacttcgagt
     1021 tggcgatcgc cgtcgccatc ggggtgttcg gcgttgcgtc gggccaggcc ctggctgggg
     1081 tcgtcggccc gctcatcgaa gtgcctgtcc tcgtcggcct ggtctatgtc gcgctctggt
     1141 tgcggggtcg ggtgcgctgg cccgaaagct ccggctgagc ccgacgggcc gggacggtcg
     1201 accggacctg cgggcgaggt cgaccgaccg gcgaccggcg ggtgcgtcac accggcgggc
     1261 acacggcata cctgggcggg taagttctgc gacgtgactg cacggccgct ggcgcacctc
     1321 gtccacccct cgtggggtga cgcgctcggg ccgttggagc cgaccgtgac cgcgctaggt
     1381 gccttcttgc gcgaggaggt ggcggccggg cgcggctacc tgccggccgg ggaacacgtc
     1441 ctgcgggcct tcacccaacc gcttgacgcg gtgcgcgtgc tcgtcgtcgg gcaggacccc
     1501 tacccgacgc ccgggcaccc ggtggggctg agtttctcgg tggcgcctga ggtgcggccg
     1561 ttgccaccga gcctggtgaa tatcttcgcc gagttgcaga ccgacctcgg cgtgccacgg
     1621 ccgagcaccg gcgacctgac gccctgggcc gatcgcggag tcctgctgct caaccgggtc
     1681 ctcaccgtcg gcccgggcaa gccgggcagc caccgcgggc ggggctggga agacgtcacc
     1741 gccgccgcca ttgccgcgct ggtcgcccgg ggcggcccgc tggtcgcgat cctctggggt
     1801 cgcgatgcgc gctcgctggc cccggcactc ggggacgtcg cccgcatcga gagcgcccat
     1861 ccctcgcccc tgtcagcccg ctccggcttc ttcggatcgc ggccgttcag tcgcgccaac
     1921 gacctgctcg tgggtcaagg cgccgagccc attgactgga gcctgccgtg agcgggctgg
     1981 agtcacgccg tcccacccag catggaggtt gcagtcatga gtgaggtctt cctctacgac
     2041 gcggtccgca cgcccttcgg ccggtatgcc ggtgcgctgg ccggcatccg acccgacgac
     2101 ctcggggcac tcgtcatccg cgagacggtc cgccgggctc cctcgctcga accggagcgg
     2161 atcgacgaga tcttcttcgg ctgcgctaac ggtgctggcg aggacaaccg caacgtcggt
     2221 cggatgtccg ggctgctcgc tggagtgccg acctcggtgc ccgcgacgac cctcaaccgc
     2281 ctgtgcgggt cgagcctgga cgccgccatg gtcgcctccc ggcagattgc cctcggcgag
     2341 gccgacgtcg tcgtcgtcgg cggggtcgag tcgatgagtc gcgccccgtg ggtgctgccc
     2401 aagaccgagc ggccctatcc cgccggctcg atggagttgg cctcgaccac gctcggctgg
     2461 cggttggtca acccggcgat gcccaaggag tggaccgtct cgctcggcga ggccaccgag
     2521 caactgcgcg agaagtatgc cgtgacccgc gaggcgatgg acgagttcgc cgcccgctca
     2581 cacttcctcg ccgatgcggc ctggtcgagc ggcttcttcg ccgatcaggt cgtcgctgtg
     2641 cccggggtcg ggctggatcg cgacgagtcg atccggcgcg agacgacggt cgagcggttg
     2701 gccgggctta agccgtcgtt ccggtccgcc gagaccggga cggtgacggc cggcaacgct
     2761 tcgccgctca acgacggtgc gtcggcagcg ctcttggggt cggccaaggc cgatgcgctc
     2821 acgggtctga gcccggtggc gcggatcgca ggtcgtgggg ccgccgcgaa cgacccgcag
     2881 ttcttcggct acgccccggt cgaggccgcc aaccaggctc tggctcgggc cggcatcacg
     2941 tgggacgacg tcgacgccgt cgaactcaac gaggcgttcg ccgctcaggc cttggcctgt
     3001 gtcgcggcgt ggggcgtgga ccccgagatc gtcaaccagc acggcggggc gatcgccatc
     3061 gggcacccgt tgggcgcatc cggcacccgg atccttggca ccctcgcccg atcgctgcag
     3121 gtcagcggcg gcaaatgggg agtcgccgcg atctgcatcg gagtcggcca aggcctcgcc
     3181 gtcgtcatcg agaacgtctc ctgaccc
//
LOCUS       DAORPW010000249         3205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_10222_length_3205_cov_822.198730, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000249 DAORPW010000000
VERSION     DAORPW010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10222_length_3205_cov_822.198730"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="PLB86_12750"
     CDS             <1..1230
                     /locus_tag="PLB86_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC66059.1"
                     /translation="LLVGLVLRQMLPRFRVMQDSIDSVNRILREQITGIRVVRAFVRE
                     PHEKARFEVANGTLTGASLGVGNLMALVFPLVMLVFNASTVAVLWFGAQRVDAGDMQV
                     GALTAFMSYLMQILMSVMMATMMSMMIPRAAVSAERIMKVLDTIPSVHPPVNPVTDIA
                     SPAAVVFESVEFRYPGAEHSVLQDISFEARPGETTAIIGSTGAGKTTLIGLIPRLVDV
                     TGGRLLVGGVDVRDADPDTLWSRIGLIPQKPYLFSGTVASTLRYGNPDATDEELWEAL
                     RIAQAEDFVRAMPEGLDAPIAQGGTNVSGGQRQRLAIARALVRRPEVYLFDDAFSALD
                     VATDARLRAALAPVTREATVIVVAQRVSTIIDAEQIIVLEDGLVVGRGRHTELLESCP
                     TYQEIVVSQRAAEEVAA"
     gene            1227..>3205
                     /locus_tag="PLB86_12755"
     CDS             1227..>3205
                     /locus_tag="PLB86_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC66060.1"
                     /translation="MSEGVKTAEEKAAEARRGPRHSGPPHMAMGMPAEKSMSFGPSAR
                     RLLSWLAPERVKVTTVLVLVVLSVVLNSVGPKILGRATDLIFAGLVGRMLGTNIPAGT
                     SRADVIAAMRAQGQDRMADMLQGVDFIPGQGIDFAAIGQVLLVVLAIYVVASVLSWLG
                     TRLLVGVVQRAMLRMREQVQAKLDRLPLAYFDNQPRGELLSRVTNDIDNVSTSLQQTM
                     SQLLNSLLTVVAMLAMMFWISWLLALIALVTIPISMVVAAAIGKRSQKLFVKQWAATG
                     QLNSIIEETFTGHQLVKVFGRQREVQEQFDAKNDELYDSAFGAQFISGIIMPVMMFVG
                     NLTYVAIAVVGGLKVASGQMTLGDVQAFIQYSRQFTQPLTQVASMANLLQSGVASAER
                     VFEVLDAPEQEAEAAYPATIAEPHGEVRFEDVSFAYTADRPLIEHLDLDVKPGHTVAI
                     VGPTGAGKTTLVNLIMRFYELDGGRITLDGVDIRDLSRQGLRERIGMVLQDTWLFAGT
                     IRDNIAYGRPGATEEEVLAASRATFVDRFVHSLPDGYDTVLDAEASNLSAGERQLVTI
                     ARAFLSEPSLLILDEATSSVDTRTELLVQKAMRALRSDRTSFVIAHRLSTIRDADVIL
                     VMEAGRIVEQGTHEELLAQHGAYARLYAAQFSGAAA"
BASE COUNT          476 a         1108 c         1050 g          571 t
ORIGIN      
        1 ctgctcgtcg ggctggtcct gcgtcagatg ctgccgcggt tccgcgtcat gcaggactcg
       61 atcgactcgg tgaaccgcat tctgcgcgaa cagatcacgg gcattcgcgt cgtgcgggcc
      121 ttcgtccgcg agccgcacga gaaggcgcgc ttcgaggtgg ccaacggcac gctcaccggc
      181 gcatcgctcg gggtcggcaa cctgatggcc ctcgtcttcc cgctcgtcat gctcgtcttc
      241 aacgcctcga ccgtcgcagt gctgtggttc ggtgcccagc gcgtcgacgc cggtgacatg
      301 caggtcggcg cactgacggc gttcatgtcc tacctcatgc agatcctcat gagcgtgatg
      361 atggcgacga tgatgtcgat gatgatcccc cgggccgcgg tgtcggccga gcggatcatg
      421 aaggtcctcg acaccattcc ttcggtccac cctccggtca acccggtgac cgacatcgcg
      481 tcgcctgcgg cggtggtctt cgagagcgtc gagttccgct accccggtgc ggaacactct
      541 gtgctgcaag acatctcgtt cgaggcccga ccgggtgaga ccactgcgat catcgggtcg
      601 acgggtgcgg gcaagaccac tctcatcggt ctcattcccc gcctcgtgga cgtcaccggc
      661 ggtcggctgc tcgtcggcgg cgtcgatgtg cgcgacgccg atccggacac cctgtggtca
      721 cgcatcggcc tgatcccgca gaagccgtac ctcttctccg gcaccgtcgc ctccaccctg
      781 cgttacggca accccgacgc caccgacgag gagctctggg aggccctgcg catcgcccag
      841 gccgaagact tcgtccgcgc catgcccgag ggtctggacg cgccgatcgc ccagggcggc
      901 accaatgtct ccggcgggca gcgtcagcgc ctggccatcg cccgggccct cgtgcgtcgt
      961 cccgaggtct acctcttcga cgacgccttc tcggcccttg acgtcgcgac cgacgcccgg
     1021 ctgcgggcgg ccctcgcgcc ggtgacgcgg gaagccaccg tcatcgtcgt cgcccagcgc
     1081 gtgtcgacca tcatcgacgc cgagcagatc atcgtccttg aggacgggct ggtcgtcggg
     1141 cgcgggcggc ataccgaatt gctcgagtcg tgtcccacct accaggagat cgtcgtgtcc
     1201 cagcgcgctg ccgaggaggt ggcggcatga gcgagggtgt gaagaccgcc gaggagaagg
     1261 cggccgaggc tcgacgcggg ccgcgccaca gcggtccccc gcacatggcc atgggtatgc
     1321 cggccgagaa gtccatgtcg ttcggcccct ccgcgcgtcg actgttgtcg tggttggcgc
     1381 ccgaacgcgt caaggtgacc accgtcctcg tgctggtcgt gctgtcggtg gtgctcaact
     1441 cggtcggccc gaagatcctc ggccgggcga ccgacctcat cttcgccggg ctcgtcgggc
     1501 ggatgctcgg caccaacatc ccggccggga cgtcccgcgc ggacgtcatc gcggcgatgc
     1561 gtgcgcaggg tcaggaccgg atggccgaca tgctccaagg tgtcgacttc atcccgggtc
     1621 aagggatcga cttcgccgcc atcgggcagg tgctgctcgt cgtgctggcg atctatgtcg
     1681 tcgcctcagt gctgtcctgg ctggggaccc ggctgctcgt cggcgtcgtc cagcgcgcga
     1741 tgcttcggat gcgtgagcag gtgcaggcca agctcgaccg tctgcccttg gcctacttcg
     1801 acaaccagcc gcgtggcgag ttactcagcc gcgtgaccaa cgacatcgac aacgtgtcaa
     1861 cgtcgttgca gcagaccatg tctcagctgc tcaactcgct gctcaccgtg gtggcgatgc
     1921 tcgccatgat gttctggatc tcgtggctgc tggccctcat cgccctggtc accatcccca
     1981 tctcgatggt cgtcgctgcg gcgatcggca agcgctccca gaagttgttc gtcaagcagt
     2041 gggcggcgac cggccaactc aactcgatca tcgaggaaac gttcaccggg caccagctcg
     2101 tcaaggtgtt cggtcgtcag cgcgaggtgc aggagcagtt cgacgccaag aacgacgagc
     2161 tctacgactc ggcgttcggg gcccagttca tcagcgggat catcatgccg gtgatgatgt
     2221 tcgtcgggaa cctcacctac gtcgccatcg ccgtcgtcgg cggcctcaag gttgccagcg
     2281 gtcagatgac cctcggtgat gtccaggcct tcatccagta ctcccggcag ttcacccagc
     2341 ccctgaccca ggtggcgtcg atggccaacc tcctgcagtc cggtgtggcc tcggccgagc
     2401 gggtgttcga ggtccttgac gcccccgagc aggaggccga ggcggcatac ccggcgacca
     2461 tcgccgagcc ccacggggag gtgcgcttcg aggacgtctc gttcgcctac accgcggacc
     2521 ggccgctcat cgagcacctc gatctcgacg tcaagccggg tcacacggtg gccatcgtcg
     2581 gcccgaccgg tgccggcaag acgaccctgg tcaacctcat catgcggttc tacgagctcg
     2641 acggtggtcg aatcacgttg gacggcgtgg atattcgtga tctgtcccgc caggggctgc
     2701 gcgagcggat cgggatggtg ctgcaggaca cctggctgtt cgccgggacg atccgcgaca
     2761 acatcgccta cggtcggccg ggcgcgaccg aggaagaggt gctcgcggcc tcgcgggcga
     2821 ccttcgtcga ccggttcgtc cactcgctgc cggacggcta cgacacggtg ctcgacgcgg
     2881 aggcatccaa cctctccgcc ggcgagcggc agttggtgac gatcgcgcgc gcgttcctct
     2941 ccgagccgtc gctgctcatc ctcgacgagg ccacctcgtc ggtcgacacg cgcaccgagt
     3001 tgctcgtgca gaaggcgatg cgggcgttgc gttcggaccg gacgtcgttc gtcatcgccc
     3061 accgcctgtc gacgatccgc gacgccgacg tcatcctcgt catggaggca gggcgcatcg
     3121 tcgagcaggg gacgcacgag gagctcctcg cccagcacgg ggcctacgcg aggttgtatg
     3181 ccgcgcagtt ctccggtgct gccgc
//
LOCUS       DAORPW010000250         3193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_10289_length_3193_cov_623.280752, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000250 DAORPW010000000
VERSION     DAORPW010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10289_length_3193_cov_623.280752"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PLB86_12760"
     CDS             complement(<1..140)
                     /locus_tag="PLB86_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRC66061.1"
                     /translation="MPQGRDVGERRPAVIAHRGASAIRPEHTLGAFRQAIRDGADAIE
                     CDV"
     gene            313..795
                     /locus_tag="PLB86_12765"
     CDS             313..795
                     /locus_tag="PLB86_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC66062.1"
                     /translation="MTLPPFQLLVDAHWRDVARLAHALVGPDDGDDVAQQAWLQAWAA
                     YPSLTSATNLRGWLFTVTYRCATDAHRGRGRRPVPVEDPALAAAERPAVDPPATASDE
                     SGVWQIVATLPPRQREAVALRYVADLDHAAIAAALGTSTAMSRRLVSDALGSLRKALS
                     "
     gene            792..1400
                     /locus_tag="PLB86_12770"
     CDS             792..1400
                     /locus_tag="PLB86_12770"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRC66063.1"
                     /translation="MTPSHSGPSDRLEASLAGFDVPAARPPDFPTADVSYAVDDTQIG
                     RMLLACDGSGVLVASLFTPDAAAEEAALQRLAARISPRVLRRPQSLDEARRWLESYLD
                     GGRPGAAPAWALSLATPFQIPVLTTLAARVGYGDRATYGQLASWAGRPQAARAVGTAL
                     GGNPLCVVLPCHRVVGASGSLTGYAGGLAAKRYLLELESTSL"
     gene            1551..2138
                     /locus_tag="PLB86_12775"
     CDS             1551..2138
                     /locus_tag="PLB86_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66064.1"
                     /translation="MTARTSPTPAPTPLDDDRVTELVTRAAYERLRSDLAVAHGTELV
                     SWDEQNPKMRGDLRESVEPTVQDTIEAIAELGFAVVPHEWLGGQASDATAVVIGEATL
                     SPAATTPNEVDIDLPASPQRLLTLASMTSRLTSSTNATAPGEQVADESEPVDPSALRG
                     LVSGAGSAAVAIVRLPQTALRPIKRVVARVMGPAA"
     gene            complement(2169..3122)
                     /locus_tag="PLB86_12780"
     CDS             complement(2169..3122)
                     /locus_tag="PLB86_12780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC66065.1"
                     /translation="MTDDVTPATDQTATAGAASLSLPDGLTSRPLGPSDVRAVFEVMA
                     AVELADTGKVAIEEADIAADWQRPSFDIAAKTIGVFDGDRMVGYGEHSGGDRSDAAVH
                     PAYRGRGVGTAVARWIQATARAAGASVVGMPVPAGSPSEALLRALGYHQRWTSWILEV
                     PQGREIASQPLPQGFSLRIAETAADHRAAWEVIEDAFLEWSERPRTPYEDWAAVTMER
                     PGFEPWNLRLAIDPSGAVVGVSLVLLADEVGYVDKLAVRKDVRNQGLARALLVDSFAA
                     ARAHGATVCELSTDSRTGALGLYEKVGMEVTSIWFNLAIDL"
BASE COUNT          461 a         1208 c         1041 g          483 t
ORIGIN      
        1 acatcgcatt cgatggcgtc cgcgccatca cggatggcct gccggaaggc gcccagggtg
       61 tgctccgggc ggatcgccga ggcgccgcgg tgggcgatca ccgccggacg acgttccccg
      121 acgtctcggc cctgcggcat ccgaccaacc tcctcgcgac gcccgacaac gacagcgcct
      181 cagcaacgac accgcctcag cagcgacgcc ttatcgtgac acggtgcctc ccaccgccgg
      241 tgctcagtcg agcgcacagt ccactggagc gccgacgact cacacctttc ggcgcccagg
      301 cgttgttctc acgtgaccct gccgccgttc caacttctcg tcgatgccca ctggcgtgac
      361 gtcgcccgat tggctcatgc cctcgtgggt ccggacgacg gcgacgacgt ggcgcagcag
      421 gcgtggctgc aggcgtgggc ggcatacccc tcgctcacct cagccacgaa cctgcgcggc
      481 tggctgttca cagtgaccta tcgctgcgcg accgacgccc atcggggacg ggggcggcgg
      541 ccggtgccgg ttgaggatcc ggccctggct gcggccgagc gcccggccgt tgacccgccc
      601 gcgaccgcct ccgacgagtc cggcgtttgg cagatcgtcg ccaccctgcc gcctcgccag
      661 cgcgaggccg tcgcgctgcg ttacgtcgcc gacctcgatc acgctgccat cgctgccgcg
      721 ctggggacct cgacggcgat gagtcgccgc ctggtcagcg acgcgctcgg ctccctccga
      781 aaggccctct catgaccccg tcacactccg gtccctccga tcgactcgaa gcatccctcg
      841 ctggtttcga cgtgcctgcg gcgcgcccac cggacttccc gaccgccgac gtgtcgtatg
      901 ccgtcgacga cacccagatc ggtcgaatgc tcttggcctg cgatggttct ggtgtcctgg
      961 ttgcatcgct gttcaccccc gatgccgccg ccgaagaggc agcgctccaa cggcttgcgg
     1021 cccgcatcag cccgcgggtg ctgcgtcgcc cgcagtcgct ggacgaggct cgacgctggc
     1081 tggagagcta cctcgacgga ggccgccccg gggccgctcc cgcgtgggcg ctctcgctcg
     1141 cgaccccgtt ccagatcccg gtcctcacga ccctcgccgc ccgcgtcggt tacggcgatc
     1201 gcgcgaccta cggccaactc gccagctggg caggccgccc gcaggccgcg cgggccgtcg
     1261 gcaccgcgct cggaggcaac ccgctgtgcg tcgtgctccc ctgccaccgc gtcgtcggtg
     1321 ccagtgggtc actcactggg tatgccggtg ggctggccgc caagcgctac ctcctggagt
     1381 tggagtccac gagtctctga cggccgcgtg cccgactctt gggtcacccc tcctcagcgc
     1441 ccgcttctac ggtgcggaat actcaggcaa ctgactttct tctcagtgac actggggaag
     1501 cgcttcgacg cccggcgggg gagccgggtc aaccgggagg aacgaacgtg atgactgcaa
     1561 ggacctcacc cacacccgcg ccgacgccgc tggacgacga tcgggtcacc gagttggtga
     1621 cgcgcgcggc atacgagcgg ctgcggagcg acctcgccgt cgcgcatggc accgagttgg
     1681 tgtcgtggga tgagcagaac ccgaagatgc gcggggacct gcgtgagtcg gtcgagccca
     1741 cggtccagga cacgatcgag gcgattgccg agttgggctt cgcggtcgtt ccgcacgagt
     1801 ggctcggcgg tcaggcgagc gacgccaccg ccgtcgtcat cggcgaagcc acgctcagcc
     1861 ccgctgcgac aacgccgaac gaggtcgaca tcgacctgcc ggccagccct cagcgtctgc
     1921 tcaccttggc gtcgatgacg tcacgcctga cctcgtcgac gaacgccacc gcgccgggcg
     1981 agcaggtcgc cgacgagagc gagcccgtcg accccagcgc cctgcgcggg ctcgtctccg
     2041 gcgccgggtc agcggcagtg gccatcgtcc ggctgccgca gaccgcgctc cgcccgatta
     2101 agcgggtcgt ggcccgggtc atgggcccag ccgcctgacc tcgcgcctca gtcgtcggca
     2161 ccctcgcctc acaggtcaat cgcgaggttg aaccagatcg acgtgacctc catgccgacc
     2221 ttctcgtaga gccccagcgc ccctgtccgc gagtcggtcg acaactcgca cacggtcgct
     2281 ccgtgcgccc tggccgcggc gaacgagtcg accaatagcg cccgcgccag gccctggttg
     2341 cggacgtcct tgcggacggc gagtttgtcg acgtagccaa cctcatcggc cagcaacacg
     2401 agcgacaccc caaccacggc accactcgga tcgatcgcca gccgcaggtt ccacggctcg
     2461 aacccgggtc gctccatggt gacggcagcc cagtcctcgt agggcgtgcg agggcgctcg
     2521 gaccattcga gaaacgcatc ctcgatcact tcccaggcgg cgcggtggtc ggctgccgtc
     2581 tcggctatcc gtagcgagaa accctgcggc agaggctgac tcgcgatctc acgtccctgc
     2641 ggcacctcaa ggatccatga ggtccaccgc tggtgatagc ccagcgcccg cagcagcgcc
     2701 tcgctcgggg agccggccgg caccggcata ccaacgacgc tcgccccagc cgcacgtgcc
     2761 gtcgcctgga tccacctcgc caccgcggtc cccaccccac gcccacggta tgccgggtgc
     2821 acagccgcgt cggagcggtc accacccgag tgctcgccgt agccgaccat gcggtcgccg
     2881 tcgaagaccc cgatggtctt ggccgcgatg tcgaagctcg gccgttgcca gtcggcggcg
     2941 atgtcggcct cttcgatggc gaccttgccc gtgtcagcta actcgacggc cgccatgacc
     3001 tcgaagaccg cccggacgtc cgaggggccg agtggccggg aggtcagccc gtcgggaagc
     3061 gagagcgagg ccgcccctgc ggtggccgtc tggtcggttg ctggggtgac gtcgtcggtc
     3121 atggccccag gatcgcaggc gcgccacgac gctgtcaggt gagtttcgcc cgcgctcggc
     3181 cgccctcgca ggc
//
LOCUS       DAORPW010000251         3192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_10293_length_3192_cov_927.244182, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000251 DAORPW010000000
VERSION     DAORPW010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10293_length_3192_cov_927.244182"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLB86_12785"
     CDS             <1..471
                     /locus_tag="PLB86_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66066.1"
                     /translation="WFAGRLPADWFIGDAQISVDREEIVVVGELPPVDGQGDSAAAAE
                     GRISRFREQSRDERIAIATQAQERYGRKVSWGARIGERRELFTHLAVPVMTRLRQPER
                     QVLDTLVDAGVARSRSEALAWAVALVGQHTETWLSDLRAKMAEVDKLRAQGPQL"
     gene            complement(557..1699)
                     /locus_tag="PLB86_12790"
     CDS             complement(557..1699)
                     /locus_tag="PLB86_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HRC66067.1"
                     /translation="MTTQPLAAPEDRFKPGLEGIVACETEIAEPDKEGSALRYRGVDI
                     EDLVDKVSFGQVWGLLVDGAFEPGLPPAEPFPLPVHSGDVRVDVQSALAQLAPIWGFR
                     PLFDIDEEEARENLARAAVMALSFVGQSARGVHKPMVPQREVDKARTIVERFMIRWRG
                     EPDPRHVEAVDAYWVSAAEHGMNASTFTARVIASTGADVAACLSGAVGAMSGPLHGGA
                     PSRVLHMLEAVENLGDATAYVKGVLDRGERLMGFGHRVYRAEDPRARVLRATCKRLDA
                     PRYEAAHALEIAALAELRERRPDRVLETNVEFWAAVILDYAEVPPSMFTPMFTCARTA
                     GWSAHILEQKRAARLIRPAARYIGIHERRPEDVPGWPRHIDPHEVH"
     gene            complement(1804..2211)
                     /locus_tag="PLB86_12795"
     CDS             complement(1804..2211)
                     /locus_tag="PLB86_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66068.1"
                     /translation="MAKSTIVGNAVKYGVKYGPHVIVAAKALKEPATAYAQKKLAEQK
                     ARRAAAAEATTLQAGTVLRVFHQGQPVWVVYSGEEPIASHPVVEATLAQLVERADLSQ
                     RFVPEPAVTAKDRITSAGGQAAGKARAALKRTR"
     gene            2267..2971
                     /locus_tag="PLB86_12800"
     CDS             2267..2971
                     /locus_tag="PLB86_12800"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HRC66069.1"
                     /translation="MTDRVDYTGEGLDEADLASTPYAQLTRWIGQAQAEGVRAAESPE
                     GLALQFATVDAAGAPNVRTVLMRFLDPRGPGFVTDAGSIKGREIAANPAVAASLGWPE
                     LFRVVRFRGLAEPIEPEALADYFSQRPWGSRVAAWASHQSEQVASRAALLAEFDRYAA
                     QFPDRGNPDDVPVPPSWSGFRIRATEVEFWAGRSSRLHDRIVFTAPSAAELGASAGQV
                     PLLDDPSAWTVSRRQP"
BASE COUNT          432 a         1106 c         1183 g          471 t
ORIGIN      
        1 tggttcgccg gccggctccc ggccgactgg ttcatcgggg acgcgcagat cagcgtcgac
       61 cgcgaggaga tcgtcgtcgt cggtgagctg cccccggtcg acggtcaggg cgacagcgct
      121 gctgctgccg agggccggat ctcgaggttc cgggagcagt cccgggacga gcggatcgcc
      181 atcgccaccc aggcgcagga gcgctacggc cgcaaggtca gctggggcgc tcgcatcggg
      241 gagcggcgtg agctgttcac ccacctcgcc gtgccggtca tgacccggct gcgccagcct
      301 gagcgtcagg tgctcgacac cctcgtcgac gccggggtcg ctcggtcgcg ctcggaggcg
      361 ctggcctggg cggttgccct ggtcggccag cacaccgaga cctggctgtc ggacctgcgg
      421 gccaagatgg ccgaggtcga caaattgcgc gcccaaggcc cgcagctgta atcgctgcac
      481 gcgcacggca taccgagaac agcaaagccg cggccccggg acggtgagtg tcccggggcc
      541 gcggcagctg taggcgtcag tgcacctcgt gcgggtcgat gtggcgcggc catccgggga
      601 cgtcctcggg ccggcgctca tggatcccga tgtagcgggc ggccgggcgg atgagccgcg
      661 cggcgcgctt ctgctcgagg atgtgcgccg accagcccgc agtgcgcgcg caggtgaaca
      721 tcggcgtgaa catcgacggc ggcacctcgg cgtagtcgag gatgaccgcg gcccagaact
      781 cgacgttggt ctcgagcacc cggtcggggc ggcgctcgcg cagctcggcc agcgcggcga
      841 tctccagggc gtgggcggcc tcgtagcgcg gcgcgtcgag ccgcttgcac gtggcgcgca
      901 gcacgcgggc ccgcgggtcc tcggcgcggt agacccggtg cccgaagccc atgaggcgct
      961 cgccgcggtc cagaacgccc ttgacgtatg ccgttgcgtc accgaggttt tcgacggctt
     1021 cgagcatgtg cagcacccgg gacggggcgc cgccgtgcag ggggccgctc atggcgccga
     1081 ccgctccgga gaggcaggcg gcgacgtcgg cgccggtcga ggcgatgacc cgcgcggtga
     1141 aggtcgaggc gttcatgccg tgctcggcgg ccgacaccca gtaggcgtcg acggcctcga
     1201 cgtgccgcgg gtcgggctcg ccgcgccagc ggatcatgaa ccgctcgacg atggtgcggg
     1261 ccttgtcgac ctcacgctgg gggaccatcg gcttgtggac gccgcgggcc gactggccga
     1321 cgaacgacag cgccatgacg gcggcccggg cgaggttctc gcgggcctcc tcctcgtcga
     1381 tgtcgaacag cggccggaaa ccccagatcg gggcaagctg ggccaacgcc gactgcacgt
     1441 cgacgcggac gtcgccggag tggaccggca gcgggaacgg ctcggcgggc ggcaggcccg
     1501 gctcgaacgc gccgtcgaca agcagtcccc atacctggcc gaacgacacc ttgtcgacga
     1561 ggtcttcgat gtcgaccccg cggtagcgca gggcgctgcc ttccttgtcg ggttcggcga
     1621 tctcggtctc gcaggcgacg atcccctcca gacccggctt gaaccggtct tcgggggcgg
     1681 cgagcggttg ggtggtcaca gggcaactcc tcggaacagt ggggcagtga agccctagcg
     1741 attctgcccc atccccccgg gcacctggga ccgcctgccc agggaacggg ccggggcagg
     1801 cgcctaacgc gtgcgcttga gcgccgcgcg ggccttgccg gccgcctgtc ctcccgcgga
     1861 ggtgatgcgg tccttggctg tcacggcagg ctcggggacg aaacgctggc tgaggtcggc
     1921 tcgctcgacg agttgggcga gggtcgcctc gacgacgggg tgggacgcga ttggctcctc
     1981 gccgctgtag acgacccaca ccggctgacc ctggtggaag acccgcagca cggtgccggc
     2041 ctgcagggtg gtcgcctccg cagccgccgc acgccgcgcc ttctgctcgg cgagcttctt
     2101 ctgggcgtat gccgtcgccg gctccttgag cgccttggcc gcgacgatga cgtgcggacc
     2161 gtacttgacg ccgtatttga ccgcattccc gacgattgtg ctcttggcca tgtgtgcctg
     2221 gacccttccc cgcgccgtct gttgacggga agcctacgct cggagggtga ccgaccgcgt
     2281 cgactacacc ggcgaggggc tcgacgaagc cgacctcgcg agcacgccct acgcccaact
     2341 cacccgctgg atcggccagg cccaggcgga aggagtccga gccgccgaat cccccgaggg
     2401 gctggcgctg cagttcgcca ccgtcgatgc tgctggcgcg cccaatgtgc ggacggtgtt
     2461 gatgcgtttc ctcgacccgc gcgggccggg tttcgtcacc gacgccgggt cgatcaaggg
     2521 ccgcgagatc gccgcgaacc ccgccgtggc ggcgtcgctc ggctggccgg agttgttccg
     2581 ggtcgtgcgc ttccgcgggc tggccgagcc gatcgagccg gaggcgctcg ccgactactt
     2641 cagccagcgg ccgtggggct cacgggtcgc cgcgtgggcg tcgcaccagt ccgagcaggt
     2701 cgccagccgg gccgcactgc tcgccgaatt cgacaggtat gccgcgcagt tccctgaccg
     2761 gggcaacccg gacgacgtgc cggtgccgcc gtcgtggtcg ggcttccgga tccgggcgac
     2821 cgaggtcgag ttctgggctg ggcgttccag ccgcctgcac gaccggatcg tgttcacggc
     2881 gccgtcggcc gctgagctgg gtgccagcgc ggggcaggtt ccgctcctgg acgacccgag
     2941 cgcgtggacg gtgtcacgcc gccagccgtg acggcatacc cctgggtttg agtcgtgggc
     3001 atgggaacgg cccgtgggga agccggtctc cgctgggcgg aggtccgacg tgcacgctgg
     3061 acgaggcgtg cctacccacg agccgcggcg actgctggaa ttgcggtcgc gtcgacgtcc
     3121 catagctggg acggtgcgct aacgcgcagc cacctcacgt gtccgatagg aactcactac
     3181 ctgaccacct cc
//
LOCUS       DAORPW010000252         3177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_10360_length_3177_cov_1056.128123, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000252 DAORPW010000000
VERSION     DAORPW010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10360_length_3177_cov_1056.128123"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="PLB86_12805"
     CDS             complement(<1..1379)
                     /locus_tag="PLB86_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRC66070.1"
                     /translation="MQALIPAAPEVALEGLNLTLVLVVLVISLIALAMGAMFRRQVLA
                     ADPGTERMQEIGAAVQEGAQAYLQRQFKTLGGFVVLVFLLLFLLPAADMGVKVGRSVF
                     FVIGALFSASIGYLGMNLAVRANVRVASAARQGDREGGMRIAFRTGGTVGMATVGLGL
                     LGASIVVLLYKGEAPKVLEGFGFGAALLAMFMRVGGGIFTKAADVGADLVGKVEAGIP
                     EDDPRNAATIADNVGDNVGDCAGMAADLFESYAVTLVAALILGSVALGVTGLVFPLIV
                     PAIGAITAIIGVFITKVKGNENGLTAINRGFYISAVISAVLSLIAAFVYLPQSTDAAI
                     LGVQIDGLRIRATMAVFLGIILAGIILWLTGYFTGTTSRPTNDVARTSLTGPATVVLS
                     GIGVGFESAVYTAVIIGAAVYGAFTLGAGSTLLALFFIALAGCGLLTTVGVIVAMDTF
                     GPVSDNAQG"
     gene            complement(1628..1969)
                     /locus_tag="PLB86_12810"
     CDS             complement(1628..1969)
                     /locus_tag="PLB86_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRC66071.1"
                     /translation="MDLALSTTAQGERTIVHVGGEIDVYTAPQLRRRLDEPIHAGCRD
                     LIVDLGRVTFLDSTGLGVLVGRLKMVRSQGGRMRIVGATDRVLKVFTITGLDRVLEMF
                     PTLADVPDDLA"
     gene            2033..>3177
                     /locus_tag="PLB86_12815"
     CDS             2033..>3177
                     /locus_tag="PLB86_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC66072.1"
                     /translation="MPTPHETRPDPAEAARADPAALLSRLASGDRQARLRHTRRIPER
                     AARTADLPSWVTPSLAGALLGEGIATLWSHQVETAEAAHGGEHVVVATGTASGKSLGY
                     LVPVLSDVLDGASAPTGRGATALYLAPTKALAHDQLDRVTRLALPGLRVATYDGDTPP
                     EERRWIREHGAYVLTNPDLVHHSLLPGHERWAPFLRALRYVVIDECHVYRGVFGSHLA
                     LVLRRLRRVAARYRSYPTFVLASATVADPGGHAARLIGMPVRPVTLDGSPRAAVTIGL
                     WEPSIRPVTDAESGVTARRRSAVAEAADLLADLVDEGVQTVAFARSRVGAEVLAQAAT
                     RRSADRGGAAADHYEAGGSRGETTADRDGTVGSGAVIAAYRGGYLPE"
BASE COUNT          543 a         1066 c         1119 g          449 t
ORIGIN      
        1 atgccttggg cgttgtccga cacggggccg aaggtgtcca ttgccacgat gacgccgacg
       61 gtcgtcaaca gcccacaccc agcgagggcg atgaagaaga gcgccaacaa tgtcgaccca
      121 gcgcccagcg tgaacgcgcc gtagacggcg gccccgatga tgactgcggt gtagacggcc
      181 gactcgaaac ccacaccgat gcccgagagg acgacggtgg ccgggccggt gagggacgtg
      241 cgagccacgt cgttggtcgg ccgggaggtc gtgcccgtga agtagcccgt cagccagagg
      301 atgatgcccg cgagaatgat gccgaggaag acggccatgg tcgcgcggat ccggagtccg
      361 tcgatctgca cgccgagaat ggcggcgtcg gtcgactgcg gcaggtagac gaatgcggcg
      421 ataagcgaca acaccgcgga gatcaccgca gagatgtaga aaccgcggtt gatggccgtc
      481 aggccgttct cgttgccctt caccttggtg atgaagacgc cgatgatggc ggtaatggca
      541 ccgatcgccg ggacgatgag cgggaacact aggccggtga cgccgagcgc cacggaaccg
      601 aggatgagcg cggcgacgag ggtcacggca tacgactcga agaggtcggc cgccatgccg
      661 gcgcagtcgc ccacgttgtc accgacgttg tcggcgatgg tggccgcgtt gcgcgggtcg
      721 tcctcgggaa tgcccgcctc gaccttgccg acgaggtcgg cacccacatc ggcggccttg
      781 gtgaagatgc cgccgccgac acgcatgaac atcgcgagca gcgccgcgcc gaagccgaag
      841 ccctcgagga ccttcggagc ctcacccttg tagagcagga cgacgatcga ggcgccgagc
      901 aggcccaggc cgacggtcgc catgccgacg gtgccgccgg tgcggaaggc gatccgcatg
      961 ccgccttcac ggtcaccctg gcgagcggcc gaggccaccc ggacgttggc gcgaaccgcg
     1021 aggttcatac cgaggtagcc gatgctcgcc gagaagagag cgccgatgac gaagaagacc
     1081 gaccgtccca ccttgacgcc catgtccgcg gcggggagga ggaagagcaa caggaagacc
     1141 aggacgacga aaccgccgag cgtcttgaac tggcgctgca ggtaggcctg cgccccttcc
     1201 tggaccgcag cgccgatctc ctgcatccgt tcggtacccg ggtcggcggc gagaacctga
     1261 cgccggaaca tcgcacccat cgccagggcg atgagggaga tgacgaggac gacgaggaca
     1321 agagtgaggt tgagcccttc gagggcgacc tcgggcgcgg caggtatcag ggcttgcatg
     1381 catttcctcc tagacgccgc ccatggcggc gcacggggtc ggcgccgaac ggggggttca
     1441 gcggaggcac atccgtgtgt tccggggcga ggctatcgcc cgcaaccgtc gcgaggcgac
     1501 agtgctggcc cgctcaggag gtgtggaacg gctcacgctg ccgatgaaaa cgcttgcagc
     1561 ccaagccaat gggaaggtgg gtcacgaccg gggcaccgcc ggtcggcacg ggcggggtcg
     1621 aggccgatca ggccaggtcg tcggggacgt ccgccagggt cgggaacatc tccaggaccc
     1681 ggtcgagccc cgtgatcgtg aacaccttga ggactcggtc ggttgcaccg acgatccgca
     1741 tccggccacc ttgggagcgc accatcttca gcctcccgac gaggacgccc agacccgtgg
     1801 aatccagaaa agtgacccgg ccgaggtcaa cgatgaggtc tcggcacccg gcgtggatcg
     1861 gctcatcgag tcgccgtcgc aattgaggtg ccgtatagac gtcgatctcc ccaccgacgt
     1921 gcacaatcgt gcgctccccc tgcgccgtgg tcgacaacgc cagatccacg atcacaacct
     1981 tccgctcact gcctttcttt gaacgttacc tcgggagcct acggtgagca caatgcccac
     2041 tccgcacgag acgcgacctg acccggccga ggcggcccgc gccgaccccg cggcgttgct
     2101 gtcgcgcctg gcgagcggcg atcgtcaggc gcggctgcgg catacccggc ggatccccga
     2161 gcgagcggcc cgcacggccg acctgccgtc ctgggtgacc ccgtccctcg cgggagcgct
     2221 cctcggcgag ggcatcgcca cgttatggag ccaccaggtc gagacagctg aggccgcgca
     2281 tggcggcgag cacgtcgtcg tcgccacggg gacagcctcg ggcaagtccc tgggctacct
     2341 cgttccggtg ctctcggacg tgcttgatgg agcatcggca cccacgggac gcggcgcgac
     2401 tgcgctgtat ctcgcgccga ccaaggctct ggcccacgac caactcgaca gggtgacccg
     2461 actcgcgttg ccggggctgc gcgtcgcgac ttacgacggc gacacgcctc cggaggaacg
     2521 gcggtggatc cgcgagcacg gggcctatgt gctgaccaac cccgacctcg tccaccactc
     2581 gttgttgccc gggcacgagc ggtgggctcc gttcctgcgc gccttgcgct atgtcgtcat
     2641 cgacgaatgc cacgtctacc gaggggtttt cgggtcgcac ctcgcgctgg tcctgcggcg
     2701 gttgcgccgg gtcgcagccc gctaccgctc ctaccccacc ttcgtcctcg cctccgcgac
     2761 cgtcgccgac ccgggtgggc acgccgctcg cctgatcggt atgccggtcc gcccggtcac
     2821 gctcgacggc tcgccacgcg cggcggtgac catcgggttg tgggagccgt cgatccggcc
     2881 ggtgaccgat gccgaatctg gggtgaccgc gcggcggcgt tcggccgtgg ccgaggctgc
     2941 ggatctgttg gccgatctgg tggacgaggg cgtgcagacg gtggccttcg cccgatcgcg
     3001 ggtcggagcg gaggtgcttg cccaggcggc cacccgccgc tcggccgatc gaggcggggc
     3061 cgccgccgat cactacgagg ccggcggaag ccggggcgag accaccgccg atcgtgacgg
     3121 aaccgtcggg tcgggagcgg tgatcgcggc ataccgaggc gggtatctgc ccgaaga
//
LOCUS       DAORPW010000253         3121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_10652_length_3121_cov_632.179713, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000253 DAORPW010000000
VERSION     DAORPW010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10652_length_3121_cov_632.179713"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLB86_12820"
     CDS             complement(<1..683)
                     /locus_tag="PLB86_12820"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC66073.1"
                     /translation="MIIDAHAHVWPDHIAAVVLATRPSGLDSHFDGTLTGLLTTMDAA
                     GIDLSMALAVANKASTVMRTNEWIGQIPRDRLIPAGTVHPGLSPEENVASLRDNGIRA
                     VKLHPLFQDVAFADPQVIDIMHALAADGVTVLTHVGSGGDAAANERGNPAALRALIDA
                     VPTLRLVAFHYGGYHQLDTAQELIVGAPVLLETSWPPSVATLDPQVVRDLIRRHGVDR
                     VVFGTDWPMT"
     gene            complement(680..1879)
                     /locus_tag="PLB86_12825"
     CDS             complement(680..1879)
                     /locus_tag="PLB86_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC66074.1"
                     /translation="MTTEGALFELPARYRQLQDEARALAESCADIAARADEADRIDPE
                     MRARLAASGLAEVTVAAAYGGRFEAVDSLAVTVVREVLAGVSAHLDSLFAMQGIGSFP
                     VSTAAAEPLRQEWLPRVARLEAIGALGLTEPDVGSDLRAITTTVRQEGDELVVDGHKS
                     FITNAGDATFYSILAREEAGYSLVFVPADTPGLSVTHPHQIIAPHVLGDVILEGVRVP
                     VGNRLGAPGAGFKLMLATLATFRVSVAGAAVGVAQAALDEAVAHASGREQFGMPLARL
                     GPVPAMLAQSWVDVESARCTAYRAAAAAARDPLSALHLSSIAKVAATEAAARVTDRSV
                     QVMGRFGLVRGSVIERLYREARPMRIYEGATEVILDSLARKLVKDAATATSAAATGSG
                     ASAGDAG"
     gene            complement(1876..3063)
                     /locus_tag="PLB86_12830"
     CDS             complement(1876..3063)
                     /locus_tag="PLB86_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC66075.1"
                     /translation="MSGFDLTAEQEQLKAVAAKVAADVYAAPAARWDLERTSLPREEV
                     ARLADLGYLGLAIPEEYGGQGGTLVDALVVIEELAKVCRPAAFQVFECNTGPIRVIQF
                     FGTEEQKQRWLTASCRGEQTMAIAISEPDAGSAATDLRTKARRDGDHWVINGAKRWIS
                     NGGHADSYLVYCRMSDQPGAKGIGAIVVPADTPGFSTGAQEKLMGFRGIPSADLFFDD
                     VRVPLDHLIIEEGGFGKLFGVFSIERLGNATMSLALGQAALDKSTAYVQERVQFGRPL
                     IDFQNVQMTIAQMAVQVEAARLLVLRASSEAGDGLPDTYQVSVAKCFANEMAKQVTDL
                     AMQLHGGNGYTEEYGLERLHRDAHGWAIAGGTPAIQRVRIASELLGRTFNQRPERAAS
                     GTP"
BASE COUNT          465 a         1048 c         1142 g          466 t
ORIGIN      
        1 gtcatcggcc agtcggtgcc gaagacgacc cggtcgacgc cgtgccgacg gatgaggtcg
       61 cggacgacct gcgggtcgag ggtggcgacc gacggcggcc acgacgtctc caacagcacc
      121 ggcgcgccga cgatgagctc ctgggctgtg tcgagctggt ggtagccgcc gtagtggaat
      181 gcgacgaggc gcagcgtcgg caccgcgtcg atgagcgcgc gcagggcagc ggggttgccg
      241 cgctcgttgg ccgcagcgtc gccaccggag cccacgtggg tgagcacggt gaccccgtcg
      301 gcggccagcg cgtgcatgat gtcgatgacc tgggggtcgg cgaacgccac gtcctggaag
      361 agcgggtgca gcttgaccgc gcgaatcccg ttgtcccgca acgaggcgac gttctcctcg
      421 ggcgacagtc cgggatgcac ggtgcccgca gggatgagcc ggtcgcgggg gatctggccg
      481 atccactcat tggtgcgcat caccgtcgag gccttgttgg cgacggccag ggccatcgac
      541 aggtcgatcc cagccgcgtc catcgtcgtc agcagcccgg tgagcgtgcc gtcgaagtgg
      601 ctgtccagcc cggacggccg ggtggccagg acgacggccg cgatgtggtc gggccacacg
      661 tgagcgtggg cgtcgatgat catccggcgt cccctgccga cgcgccgctg ccggtcgcag
      721 cggccgaagt cgccgtcgcc gcatccttga cgagtttgcg ggccaggctg tcgaggatga
      781 cctcggtcgc gccttcatag atccgcatcg gccgcgcctc gcggtagagc cgctcgatga
      841 cgctgccgcg caccaacccg aaccgcccca tcacctgcac cgagcggtcg gtgacgcggg
      901 ccgcagcttc cgtcgcggcc accttggcga tcgaggagag atgcagggcc gacaacggat
      961 cgcgcgcggc agccgcagcc gcccggtatg ccgtgcaccg cgccgactcg acgtcgaccc
     1021 agctctgcgc gagcatcgcg ggcacggggc cgaggcgcgc gagcggcata ccgaattgct
     1081 cgcgtccgga tgcgtgcgcg acggcctcgt cgagggcggc ctgggccacc ccgacagcgg
     1141 cgcccgcgac cgagacccgg aaggtcgcca gcgtcgcgag catgagcttg aaacctgccc
     1201 cgggtgcgcc caggcggttg ccgaccggca cccgaacgcc ctcgaggatg acgtcgccga
     1261 ggacgtgcgg ggcgatgatc tggtgggggt gcgtgacgct gaggccagga gtgtccgcgg
     1321 ggacgaacac cagtgaatag ccggcctctt cgcgcgcgag gatgctgtag aacgtcgcat
     1381 caccggcgtt ggtgatgaac gacttgtgcc cgtcgacgac gagttcgtcg ccctcctggc
     1441 ggaccgtcgt cgtgatcgcg cgcaggtcgg agccgacgtc gggctcggtg aggccgagcg
     1501 cgccgatcgc ttcgagccgg gcgacccgcg gcaaccactc ctggcgcagc ggctcggcgg
     1561 ccgcggtcga cacggggaaa ctgccgatgc cctgcatcgc gaagagcgag tcgaggtggg
     1621 cgctcacgcc cgccagcacc tcgcggacca cggtcaccgc aagtgagtcg acggcttcga
     1681 accgcccgcc gtatgccgcc gcgacagtga cctcggccag cccggaggcg gccagtcggg
     1741 cgcgcatctc ggggtcgatc cggtcggcct cgtcggcgcg ggcggcgatg tcggcgcagg
     1801 actcggccag ggcgcgggcc tcatcctgca actgccggta gcgggccggc agttcgaaga
     1861 gggcgccttc ggtggtcacg gggtgccgct ggcggcgcgc tccggccgct ggttgaacgt
     1921 gcgcccaagc aactcggagg cgatccggac ccgctggatc gccggagtgc ctccggcgat
     1981 ggcccagccg tgggcgtcgc ggtgcagccg ttccaagccg tactcctcgg tgtagccgtt
     2041 gccgccgtgc aactgcatcg ccaggtcggt gacctgcttg gccatctcgt tggcgaagca
     2101 tttggcgacc gacacctggt aggtgtcggg cagcccgtcg cccgcctcgc tggaggcccg
     2161 caggacgagc agtcgtgccg cttcgacctg cacggccatc tgcgcgatgg tcatctggac
     2221 gttctggaag tctatgagcg ggcgcccgaa ctgcacgcgt tcctggacat aggcggtgga
     2281 cttgtccagc gccgcctgac cgagggccag gctcatcgtc gcgttgccga gccgctcgat
     2341 cgagaagacc ccgaagagct tgccgaagcc gccctcctcg atgatcaggt ggtccagggg
     2401 cacgcggacg tcgtcgaaga acaggtcggc actgggtatg ccgcggaagc ccatgagctt
     2461 ctcctgggca ccggtcgaga agccgggagt gtcggcgggg acgacgatcg cgccgatgcc
     2521 cttggcgccc ggctggtcgg acatccggca gtagacgagg tagctgtcgg cgtggccacc
     2581 gttggagatc cagcgcttgg cgccgttgat gacccagtgg tcgccgtcgc ggcgggcctt
     2641 ggtgcgcagg tcggtcgcgg ccgatccggc gtcgggctcg gagatggcga tggccatcgt
     2701 ctgctcgccg cggcacgagg cggtcagcca gcgctgcttc tgctcctcgg tcccgaagaa
     2761 ctggatgacc cggatcggcc cggtgttgca ctcgaagacc tggaaggcgg ccggacggca
     2821 taccttggcc agttcttcga tgacgacgag agcgtcgacg agggtgccgc cctggccgcc
     2881 gtactcctcg gggatggcca aaccgaggta accgaggtcg gcgaggcgag caacctcctc
     2941 acggggaagc gacgtgcgct cgaggtccca gcgggctgcc ggggcggcat agacgtccgc
     3001 tgcgaccttg gccgctaccg ccttgagctg ctcctgctcc gcggtgagat cgaacccact
     3061 cacgctggcc tccttgatca cggcttgatg ataactatga tcaccatagc gatgaattgc
     3121 c
//
LOCUS       DAORPW010000254         3106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_10745_length_3106_cov_693.006555, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000254 DAORPW010000000
VERSION     DAORPW010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10745_length_3106_cov_693.006555"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      3..114
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            130..1302
                     /locus_tag="PLB86_12835"
     CDS             130..1302
                     /locus_tag="PLB86_12835"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRC66076.1"
                     /translation="MTVSDDPPFVTGAWRDGDPVGHRAFVDFPELHLERGGVLPSVRV
                     AYETWGTLNPAGSNAILVEHALTGDSHVVGAAGPGHASPGWWEGLVGPGRDIDTDEWF
                     VVAANVIGGCQGSTGPASPAPDGRPWGSRFPFVTIRDQVAAEAALADALGIEAWALVI
                     GGSMGGMRVIEWAVTYPDRVAQAVILASTAAATADQIAWAQPQLLAIRSDPDFAGGDY
                     YGHGRGPDVGLGIARRIAQATYRSASELDERFGREPQSEEDPLGGGGRYAVESYLDYH
                     SEKLRRRFDANSYLVLTEAMNSHDVGRDRGGVAAALARVTAHVIAVGVDSDRLYPVAQ
                     SAQIARGVRRSDLRTIHSPCGHDGFLIEVDQVGALVRDALRGVEGTQGRHDSAAIR"
     gene            1345..1752
                     /locus_tag="PLB86_12840"
     CDS             1345..1752
                     /locus_tag="PLB86_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC66077.1"
                     /translation="MAVVVGYVATKEGRAALRRAAEECELRHTRLIVINSQRGGKDFD
                     ADEAAKFEAELETIQKKLTDLGLQNEVRQLVRGNEPAEDLIAVAEEENAEFIVIGLRR
                     RTPVGKLILGSNAQRILLDATCPVLAVKASRED"
     gene            2092..>3106
                     /locus_tag="PLB86_12845"
     CDS             2092..>3106
                     /locus_tag="PLB86_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HRC66078.1"
                     /translation="MTGADVREALEAAQVPPRRMKAVLRAIEEHGITVSIDAPTRAVA
                     ATAKRTVSTVSAHSEAAASAPTKTAAKKAAPAKTAEPTAAAKAPTATKTTAAAKGAAA
                     KTAAAPVAATGAVKAATKTAAPRASAAKAAAATAGDAAAANGAGAAPVKASARTRPTK
                     AAAAAASVAAEAGTGEAAPAPATRKRASRAASPAAPKGGARAATDAPEGEDIEAIDGD
                     EIIVADEAAVDEVELVEVVEVDADEAESDTEAEDGDEETPKKPAEEESEDAGFVIRDD
                     DEEDAPAQQVVTAGATADPVKDYLKQIGKVALLNAEQEVELAKRIEAGLFAEEKLNSG
                     EKLD"
BASE COUNT          506 a         1071 c         1111 g          418 t
ORIGIN      
        1 caggtcatga gtgtcagcga caagccccgg ctcgctgacc ggcaaccctt cttcgtgatg
       61 gggtgccccg ggtgaagacc cggcccgcgc gtcccgctcg ggcaagcgcg agcgtgagga
      121 gtttcgtccg tgaccgtcag cgacgacccg ccgttcgtca cgggcgcgtg gcgcgacgga
      181 gacccggtcg ggcacagggc tttcgtcgac ttccccgaac tgcaccttga gcggggaggc
      241 gtcctgccgt cggtccgggt cgcctacgag acgtggggga cgctgaaccc ggcgggaagc
      301 aacgccatcc tcgtcgaaca cgccctcacg ggggacagcc acgtcgtcgg tgctgctggg
      361 ccggggcacg catcccccgg ctggtgggag ggcctggtcg ggcccggtcg cgacatcgac
      421 accgacgagt ggttcgtcgt cgcggccaac gtcatcggtg gatgccaggg cagcaccggc
      481 cccgcctcgc ccgccccgga cggccggccc tgggggagtc ggttcccgtt cgtgacgatc
      541 cgcgatcagg tggctgccga ggcggctctg gccgatgccc tcgggatcga ggcctgggcc
      601 ctggtcatcg gcggatcgat gggcgggatg cgggtcatcg agtgggcggt gacctacccg
      661 gaccgggtgg cacaagcggt gatcctggcg agcacggcgg cggcgaccgc cgaccagatc
      721 gcctgggctc agccacaact gctcgcgatc cggtccgacc cggacttcgc cgggggcgac
      781 tactacggtc acggccgggg gccggacgtc gggctgggga tcgctcgacg gattgcccag
      841 gcgacctatc gcagcgcctc cgagttggac gagcgcttcg gtcgggaacc gcagtccgag
      901 gaagaccccc tcggcggggg cggccgatat gccgtcgaga gttacctgga ctatcacagc
      961 gagaaactgc gccgtcggtt cgacgccaac tcctacctcg tgctcaccga ggcgatgaac
     1021 tcgcacgacg tcggccggga ccgcggcggg gtggcggccg cgctggcccg agtgacggct
     1081 cacgtgatcg ccgtcggcgt cgattccgac cggctctacc cggtggccca atccgctcag
     1141 atcgcccggg gggtccgacg cagtgacctg cggacgatcc actcgccctg cggccacgac
     1201 ggcttcctca tcgaggtcga tcaggtcggg gccttggtcc gtgacgcact tcgcggtgtc
     1261 gagggcaccc aggggcggca cgattcagcc gctatccggt aacgtcgcac tcaggacgcg
     1321 cccggcgtca acaaggaggt tacggtggcc gtcgttgtcg gatacgttgc gaccaaagag
     1381 gggcgagctg ccctccgtcg cgcggccgag gagtgcgaac tgcgtcatac ccgcctgatc
     1441 gtcatcaact cccagcgggg tggcaaggac ttcgacgctg atgaggccgc gaagttcgag
     1501 gcagagctcg aaaccatcca gaagaagctg accgatctcg ggcttcagaa cgaggttcgt
     1561 cagttggttc gcggaaacga gcctgccgaa gacctcatcg ccgtcgccga ggaggagaac
     1621 gccgagttca tcgtcatcgg ccttcgtcgg cgcacccccg tcggcaagct catcctcggg
     1681 tcgaacgcgc agcggattct gctcgacgcc acctgccctg tgctggccgt caaggcctcg
     1741 cgcgaggact gacattcggg gcgacagacg gtgagaagcg gggccctcga cggacgcggt
     1801 taacgtcgag ggacgcccgc gttttataat gtcgagtatc ggggctacgc ggcctcgggc
     1861 gacactgcct cccaagggtt ccccgctccg ccgcgccccc tcccactcag ctcactccgt
     1921 cccgccgtat gccgtcccgg ggaggtcggt gtgcgctcgc atggcgaaag gtagtcggtg
     1981 cccgtgtcgc acgtctcctc cagttcccgg ggcgccgatc gcgctctgcc gacggagttc
     2041 tcacatccgc aactgcagaa gctgatcgct cgggggtcca ccatgggtag cgtgactggc
     2101 gcggatgtcc gtgaagcact ggaggccgcg caggtgccgc cccgtcggat gaaggccgtg
     2161 cttcgagcga tcgaagagca cgggatcacc gtgtccatcg atgcgccgac ccgcgccgtc
     2221 gctgccaccg cgaagcgaac cgtgtcgacc gtctctgcgc acagcgaggc ggccgcgtcg
     2281 gcaccgacca agaccgcggc caagaaggcc gcgccggcaa agactgcgga gccgaccgct
     2341 gcggcgaagg caccgacggc gaccaagacc acggcagccg ccaagggtgc cgcagcaaag
     2401 accgctgctg ccccggtggc ggcgaccggt gccgtcaagg ccgccaccaa gacggccgcg
     2461 ccgagggcga gcgcagccaa ggcggcagcg gcgaccgctg gagacgccgc tgcggcgaat
     2521 ggggctggcg cagcgccggt caaggcgtcg gcccggaccc gcccgaccaa ggcagcggca
     2581 gccgcggcct ccgtggctgc tgaggctggc acgggcgagg ccgcgccggc ccctgcgacc
     2641 cgtaagcgcg cctcgcgcgc agcgagcccc gccgcgccca agggcggcgc gcgcgctgcg
     2701 acggacgccc ccgagggcga ggacatcgag gccatcgacg gggacgagat catcgtcgcc
     2761 gacgaggcgg ccgtcgacga ggtcgagttg gttgaggtcg tcgaggtcga cgccgatgag
     2821 gccgagagcg acaccgaggc cgaggacggc gacgaggaga cccccaagaa gcccgccgag
     2881 gaggagtccg aggacgccgg cttcgtcatt cgtgacgacg acgaggagga cgctcccgcc
     2941 cagcaggtcg tcaccgccgg cgccaccgcc gacccggtca aggactacct caagcagatc
     3001 ggcaaggtcg ccctcctcaa cgctgagcag gaggtcgagc tcgccaagcg catcgaggcc
     3061 ggcctgttcg ccgaggagaa gctcaactcg ggcgagaagc tcgaca
//
LOCUS       DAORPW010000255         3103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_10767_length_3103_cov_990.043963, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000255 DAORPW010000000
VERSION     DAORPW010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10767_length_3103_cov_990.043963"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..644
                     /locus_tag="PLB86_12850"
     CDS             72..644
                     /locus_tag="PLB86_12850"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRC66079.1"
                     /translation="MRLVIMGPPGAGKGTQAERIAEQFGIPAISTGAIFRANVKGQTE
                     LGKQVEAIMATGGYVPDELTNALVRDRLAEADCAPGFLLDGYPRTVGQLHTLDAHLEE
                     TGRQIDAALELVVDEDAMVARLLARAADQGRADDTEEVIRERMSIYRAATAPLTAIYG
                     DQGKLRQVDGMGDIDEITARIVAALADLQG"
     gene            649..1494
                     /gene="map"
                     /locus_tag="PLB86_12855"
     CDS             649..1494
                     /gene="map"
                     /locus_tag="PLB86_12855"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRC66080.1"
                     /translation="MSARRKTPGRTDDQLLLMRAAGLLVGQTLEVVAAAVADGVTTLE
                     LDRLAEAHIRDHGGRPSFPEVPGYRHTLCTSVNEQIVHGIPGERALRAGDLVSIDCGA
                     IVAGWHGDAAVTVIVGGVEAARPEDLALSRATEDSLYAGIAALRVGERLYAVGEAVEA
                     CIEAAAAANGQTYGIVEDYVGHTIGTEMHQDPQIFNYAVRTRGPMVRSGYTGAIEPMV
                     TLGSAESDVLADGWTVVTSDGSRAAHWEHTVAVRDEGLWVLTAIDGGAARLAALGAAF
                     APLSD"
     gene            1507..1893
                     /locus_tag="PLB86_12860"
     CDS             1507..1893
                     /locus_tag="PLB86_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRC66081.1"
                     /translation="MGTVTDYLATVDAARRPALERVVARAHDLVPGLVEGTAYGMPVL
                     LHRGKPLLSAVEAKAHLALYPHSGVIVDLVAADLDGFSLSKGTIRFQTDQPIPDAVLD
                     RVITLRRQHIDEALDTPRPKRARGNA"
     gene            2062..2283
                     /gene="infA"
                     /locus_tag="PLB86_12865"
     CDS             2062..2283
                     /gene="infA"
                     /locus_tag="PLB86_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRC66082.1"
                     /translation="MAKKDGVIEIEGTIVEALPNATFRVELSNGHKVLAHISGKMRQH
                     YIRILPEDRVVVELSPYDLSRGRIVFRYR"
     gene            2402..2515
                     /gene="rpmJ"
                     /locus_tag="PLB86_12870"
     CDS             2402..2515
                     /gene="rpmJ"
                     /locus_tag="PLB86_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRC66083.1"
                     /translation="MKVQPSVKKICDKCKVIRRNGRVMVICENLRHKQRQG"
     gene            2781..>3103
                     /gene="rpsM"
                     /locus_tag="PLB86_12875"
     CDS             2781..>3103
                     /gene="rpsM"
                     /locus_tag="PLB86_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019174662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRC66084.1"
                     /translation="MARLIGVDLPRDKRVEIGLTYIFGVGRTTASKALVATGISPDTR
                     VKNLTDADLVSLRDWLETNVKMEGDLRREVAADIRRKVEIGSYEGIRHRRGLPVRGQR
                     TKTNAR"
BASE COUNT          520 a         1112 c         1032 g          439 t
ORIGIN      
        1 ggcgtcggcc tcgagacggt gaagcagatc caggctcagc tgcagcagcg caactacgaa
       61 gggttcctcc gatgaggttg gtcatcatgg gcccccccgg ggcaggcaag ggcactcagg
      121 ccgagcgcat cgccgagcag ttcggcatcc cggccatctc gacgggcgcg attttccgcg
      181 ccaacgtcaa gggccagacc gagctgggca agcaggtcga ggcgatcatg gcgaccggcg
      241 gctacgtccc tgacgagctg accaacgcgc tggtccgcga ccggttggcc gaggccgact
      301 gcgctcccgg cttcctgctg gacggctacc cccgcacggt gggtcagctg cacactctcg
      361 acgcgcacct cgaagagacc ggccgccaga tcgacgccgc tctcgagctc gtcgtcgacg
      421 aggacgccat ggtggctcgc ctgctcgctc gggcggccga ccagggccgg gccgacgaca
      481 ccgaagaggt catccgcgag cggatgagca tttaccgggc ggccaccgcg ccgctgaccg
      541 ccatctacgg cgaccagggc aagttgcgcc aggtcgacgg gatgggcgac atcgacgaga
      601 tcaccgcgcg catcgtcgcc gcgctggccg acctgcaggg ctgatcgctt gtctgctcgt
      661 cgcaagacgc ccgggcgcac cgacgaccag ttgctgctca tgcgggccgc cggcctcttg
      721 gtcggacaga cccttgaggt ggtcgcggct gctgtcgctg acggcgtgac gaccctcgaa
      781 ctcgaccgtc tcgccgaggc tcacatccgt gaccacggcg ggcgaccgtc cttccccgag
      841 gtgccgggct accggcatac cctctgcacg tccgtcaacg agcagatcgt ccacggcatc
      901 ccgggggagc gtgccctgcg cgccggtgac ctcgtgtcca tcgactgcgg ggcgatcgtc
      961 gccggctggc acggcgatgc ggccgtcacc gtcatcgtcg gcggcgtcga agcggcccgc
     1021 cccgaggacc tcgcgctctc ccgcgcgacc gaggactcgc tgtatgccgg catcgccgcg
     1081 ctgcgggtcg gggagcgtct gtatgccgtc ggcgaggccg tcgaggcctg catcgaggcc
     1141 gccgcggcgg ccaacgggca gacgtacggc atcgtcgagg actacgtcgg ccacaccatc
     1201 gggaccgaga tgcaccagga cccgcagatc ttcaactacg ccgtgcggac ccgcggcccc
     1261 atggtgcgct cgggctacac cggggccatc gagcccatgg tcaccctggg ttccgccgag
     1321 agcgacgtgc tggccgacgg ctggaccgtc gtcacgtccg acggctcgcg ggcggcccac
     1381 tgggagcaca cggtcgccgt ccgtgacgag ggcctgtggg tcctcaccgc gattgacggt
     1441 ggcgcggccc gattggcggc cctgggtgct gcgttcgccc cgctgagcga ctaggagagc
     1501 gtcgatatgg gcacggtcac cgactacctg gccaccgtcg acgctgctcg acgcccggcc
     1561 ctggagcgcg tcgtcgcccg cgcgcacgac ctcgtgcccg ggctggtcga gggcacggca
     1621 tacgggatgc ccgtcctgct gcaccgcggg aagccgctgc tgtctgcggt ggaggccaag
     1681 gcgcacctcg cgctctaccc ccacagcggc gtcatcgtcg acctcgtggc ggccgacctc
     1741 gacgggttca gcctctccaa gggcacgatc cggttccaga ccgaccagcc gatccccgac
     1801 gccgtcctgg accgggtgat cacgctgcgg cgccagcaca tcgacgaggc gctggacacc
     1861 ccgcgcccca agcgagcccg cgggaacgcc tgaccggctc ggcccacccg gcgccggacg
     1921 cacccgcgat ttctggtatg ccgcgcgctt gccgtaggat cgtccgttgg gtgtcgtgtg
     1981 ctcgcgactc ccgggtggaa cgcgtcattg tggcgtgccc accttcgacc cgggcacacg
     2041 gacaacggaa cgacggagga catggccaag aaagacgggg tcatcgagat cgaaggcacg
     2101 atcgtcgagg cactaccgaa cgcaacgttc agggtggagc tctctaacgg tcacaaagtg
     2161 ctcgctcaca tctcgggcaa gatgcgccag cactacatcc ggatcctccc cgaggaccgg
     2221 gtcgtggtgg aactctcgcc gtatgacctg tcccgcgggc gcatcgtctt ccgctaccgc
     2281 tgactgtttc gcaccacccg ccctcggcgt ccgccgatgg cactcccgat cccctgcaac
     2341 ggatcatggg caggcctgtg cctggccgac ccgttgcacc cgatctgaaa gacacgaggc
     2401 aatgaaggtc cagccgagcg tcaagaagat ctgcgacaag tgcaaggtga tccgccgtaa
     2461 cggtcgggtc atggtcatct gcgagaacct gcgccacaag cagcgccagg gctgatcgcg
     2521 gccgaccggc caagcacgac tcgcgggctt ccacagcacg cagcaagtag cacaactgaa
     2581 cacgcacgca gcacccggcc cctgccggaa gcctgacctc agcaccctcg cgggtggcca
     2641 gggccaggac cccgacagga cgtcaccccc ggcacggagg ccggggaccg gaccagcgca
     2701 gacccgcgag ggcgacgcgc acggcatacc ggatcggtgc tgcaccagac ctccgcaacc
     2761 gatcaggagc acagcaccag atggcacgtc tcatcggcgt ggatctcccg cgtgacaagc
     2821 gcgtggagat cggcctgacc tacatcttcg gagtgggtcg cactaccgcg agcaaggccc
     2881 tcgtggcgac cgggatcagc ccggacaccc gggtcaagaa cctcaccgac gccgacctcg
     2941 tctcgttgcg tgactggctc gagaccaacg tcaagatgga aggtgacctc cgccgtgagg
     3001 tcgccgcgga tatccgtcgc aaagtcgaga ttggctccta cgagggaatt cggcaccgtc
     3061 gtggcctgcc tgttcgcggg cagcgcacca agaccaacgc gcg
//
LOCUS       DAORPW010000256         3064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_10963_length_3064_cov_819.134596, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000256 DAORPW010000000
VERSION     DAORPW010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10963_length_3064_cov_819.134596"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1441
                     /locus_tag="PLB86_12880"
     CDS             230..1441
                     /locus_tag="PLB86_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRC66085.1"
                     /translation="MAQLTSRLRGRKALTGIVVLAVLLAAGATWWVRSRSTASAASAV
                     PTSAVAASVQTLSQSVSATGTINPKVQSNLRFGSSGTVAAVDVKVGDVVTAGQQVAAI
                     TTTDLQTALDLAEANVSSAQANLTTVKASSATTTSQLNAAKSQLAAAQAKLTTAQTNL
                     TNARLTSPVAGTVATVNIAVGDQVSAGGTSSGGTGSSGSGSSGAASAAIVVITTDAWI
                     VSTSVTSADLPLLKQGMQAQITPSGSRTLVFGTVSTIGVIATSTSGVATFPVTIAVTG
                     SPKGLYAGSSASVAIIVKEVADAITVPTAAIRTEGGKTVVSKLVDGAAVTTPVQVGLV
                     QGSFTQVTSGIAEGDQVVVTGFSGPGSGSGGQSTSTRTRGSGGAGGLGGVGFPPGGQG
                     DLPAPPPGAVP"
     gene            1516..2196
                     /locus_tag="PLB86_12885"
     CDS             1516..2196
                     /locus_tag="PLB86_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC66086.1"
                     /translation="MFELRGVSKTYTSGSLDVAAVRNVDLVVNHGDYVAIMGPSGSGK
                     STLMHIIGCLDVPTSGSYWLAGVDVAGMGESDLAVVRNQRIGFVFQQFNLLPTMSAWR
                     NVELPLIYAGVSRAERRRRAIDALTRVGLADRVDHRPGELSGGQQQRVSVARALVTEP
                     DLILADEPTGNLDSTSTAEMLELFAELNQQGRTIVLITHEHDVAEAADRMVVMRDGSL
                     DEPTGARR"
     gene            2193..>3064
                     /locus_tag="PLB86_12890"
     CDS             2193..>3064
                     /locus_tag="PLB86_12890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC66087.1"
                     /translation="MTWLETLRTAYDSLRTRRLRSALTMLGILIGIAAVMLTVGLGQG
                     AQKQVTDQINKLGSNLLIVTPGSTTSSSGVRGGRGSATTLTIDDAAVLADPSVAPDIA
                     RVAPTSGGSQSLVAGTTTWTSNVVATNAEWLGVRARSVAQGRFFTADEVTSESSVAVL
                     GSTTAAQLLGSTNAVGQTITVGGQNVTVIGVLNTEGSTTGGDQDDLVVMPLTTYASRL
                     STSANPRSVSTIYIEAKDATSLSAAYQETTNALLSAHGVTLATQDFSVSSQQAIVETA
                     TTTTRILTVLLGSIA"
BASE COUNT          490 a         1105 c         1023 g          446 t
ORIGIN      
        1 gcgaccgtgg cgttgggttc cgccgaccac acgaggtcgg aagcggcata ccggtcgtcc
       61 cacagcgtcg cgtccatgac ggcgattctc gcgcacagcc acgcggcacg aaacgcacag
      121 gtgacacaca ggtttcgctc ggttgggtcc aaggttcctg tggtggattg agccggagtc
      181 gcgaaccacg ccagtagcat gctggccgta gcgcaaccgg gaaggtgcca tggcccagct
      241 cacgtccagg ttgcgaggcc gcaaggccct gaccggcatc gttgtcctcg cagttctcct
      301 ggccgccggc gcgacctggt gggtgcgttc gcgctcgacc gcgtccgccg cctcagccgt
      361 gccgacctcg gcggtggcag catcggtgca gaccctcagc cagtcggtga gcgcgaccgg
      421 gacgatcaac cccaaggtcc agtccaacct gcggttcggc tcgtccggca cggtggcggc
      481 ggtcgacgtc aaggtcggcg atgtcgtgac cgcgggccag caggtcgccg cgatcaccac
      541 caccgatctg cagactgccc tcgacttggc cgaggccaac gtctcctcgg cgcaggccaa
      601 cctcacgacg gtcaaggcca gcagcgccac gacgaccagc cagctcaacg ccgcgaagtc
      661 ccagctcgcc gccgcccaag cgaaactgac caccgcccag accaacctca ccaacgccag
      721 gctcaccagc ccggtcgcgg gcacggtcgc taccgtcaac atcgccgtcg gcgaccaggt
      781 gagtgccggc ggcacgtcct caggcggcac cggctcgtcc ggctccgggt catcgggcgc
      841 cgccagcgcg gcgatcgtcg tcatcaccac cgacgcctgg atcgtgtcga cctcggtgac
      901 ctcggccgac ctgccgctgc tcaagcaggg gatgcaggcc cagatcacgc cgtccggctc
      961 gcggacgctc gtcttcggca cggtctcgac gatcggggtc atcgcgacct ccaccagcgg
     1021 ggtcgcgacc ttcccggtca ccatcgcggt caccggctct cccaagggct tgtatgccgg
     1081 gtcgtcggcg agcgtggcca tcatcgtcaa ggaggtcgcg gacgcgatca ccgtgccgac
     1141 ggcggccatc cgcaccgagg gcggcaagac tgtcgtcagc aagctcgtcg acggggccgc
     1201 cgtgaccacg cccgtgcaag taggcctggt tcagggcagc ttcacccagg tgaccagtgg
     1261 gatcgccgag ggcgaccagg tcgtcgtcac cggcttctcg gggccggggt ccggctcggg
     1321 cgggcagagc accagcaccc gcactcgcgg atcgggtggg gccggaggac ttggtggcgt
     1381 cggcttccct cccggtgggc agggcgatct gcccgcgccg ccgcctgggg cagtgccgtg
     1441 accgccgaga ctccgtgggc gaccgatctc gccggagacg ccgtcgccgt tgagcgaccg
     1501 ttgaccgacg gcgccctgtt cgagctgcgc ggcgtcagca agacctatac ctcgggttcg
     1561 ctggacgtgg ccgcggtccg gaacgtcgac ctcgtcgtga accacggcga ctacgtcgcg
     1621 atcatgggcc cctccgggtc cggcaagtcg accctcatgc acatcattgg gtgcctcgac
     1681 gtgccgacgt ccggaagtta ctggctggcc ggggtcgacg tcgccgggat gggcgagagc
     1741 gacctcgccg tcgtgcgcaa ccagcggatc ggcttcgtct tccagcagtt caacctgctc
     1801 ccgacgatgt cggcctggcg caacgtcgag ctgcccctca tctatgccgg ggtgtcgcgt
     1861 gccgagcggc gccggcgggc catcgatgcg ttgacccggg tcggcctggc cgaccgggtc
     1921 gaccaccggc cgggtgaact ctccggcggg cagcagcagc gggtgtcggt ggctcgcgcc
     1981 ctggtgaccg agcccgactt gatcctcgcc gacgagccca ccggcaacct cgactccacg
     2041 tccaccgccg agatgctcga acttttcgcc gagctcaacc agcagggtcg cacgatcgtc
     2101 ctcatcaccc acgaacacga tgtcgccgag gccgccgatc ggatggtcgt catgcgcgac
     2161 ggcagcctgg atgagccgac aggagcacgt cggtgacctg gctggaaacc ctgcgcacgg
     2221 catacgactc cctgcggacc cgccgcctgc ggtcggcgct gacgatgttg ggcatcctca
     2281 tcgggatcgc cgcggtcatg ctcaccgtgg ggttgggcca gggcgcccag aagcaagtga
     2341 ccgaccagat caacaagctt ggctccaacc tgctcatcgt cacgccgggc agcacgacga
     2401 gctcctcggg cgtgcgcggt ggacggggaa gcgcgaccac cctgaccatc gatgacgcgg
     2461 ccgtgcttgc cgacccgagc gtggcgccgg acatcgcccg ggtcgcgccg accagcggcg
     2521 gctcgcagag cctggtggcc ggcaccacga cgtggacgag caatgtcgtt gcgacaaacg
     2581 ccgagtggct cggcgtccgg gcccgctcgg tggcacaggg gaggttcttc accgccgacg
     2641 aggtcacctc ggagagttcg gtcgccgtgc tcggctcgac caccgccgcc caactgctcg
     2701 gctcgaccaa cgccgtcggc cagaccatca cggtgggcgg gcagaacgtc acggtcatcg
     2761 gcgtgctgaa caccgagggc tcaaccaccg gtggtgacca ggacgacctg gtcgtcatgc
     2821 cgttgacgac ctatgcctca cgcctgtcca cgagcgccaa cccccgcagc gtgtcgacga
     2881 tctacatcga ggcgaaggac gcgacgagtc tctccgcggc ataccaggag acgaccaacg
     2941 cgctgctatc ggcgcacggc gtcacgcttg ccacccagga cttcagcgtg agcagccagc
     3001 aggcgatcgt cgagaccgcg accaccacca cgcggatcct caccgtcctg ctggggagca
     3061 tcgc
//
LOCUS       DAORPW010000257         3031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_11175_length_3031_cov_679.012097, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000257 DAORPW010000000
VERSION     DAORPW010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11175_length_3031_cov_679.012097"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLB86_12895"
     CDS             complement(<1..215)
                     /locus_tag="PLB86_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HRC66088.1"
                     /translation="MATAQDKGKALASVMGQIEKAHGKGAVMRLGDDVRPPIEVIPTG
                     SIALDIALGIGGLPRGRVVEIYGPESSG"
     gene            complement(478..1299)
                     /locus_tag="PLB86_12900"
     CDS             complement(478..1299)
                     /locus_tag="PLB86_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC66089.1"
                     /translation="MSRLRRVPRAVGGALPRPAGAGLARTLVERNILSFRRQWVAFAT
                     GFAEPVFYLFSLGIGLGALVGSVTTDAGHSVSYAVFVAPAMLATSAMNAGVMDSTFNV
                     FFKLKYAKLYDSVLATPMGPRDVAVGEVTWSLLRGGAYAAGFLVVAALAGVLPSWWGL
                     LALPAAVFVAFAFSAVGMFATTYMRSWVDFDYVNLVVQPMFLLSATFFPLSAYPGWAQ
                     WLVEATPLYHGVALCRALCLGEVGVGLLWHVAYLGVMGAFGVVGAARRVDVLLLR"
     gene            complement(1296..2180)
                     /locus_tag="PLB86_12905"
     CDS             complement(1296..2180)
                     /locus_tag="PLB86_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC66090.1"
                     /translation="MDHPPARDERTQPHGIPKRGYAAPAPEPRAILTATRRWVPIVEF
                     HLRVMRRVWRGMLYSRLGTPLLSLLAVGWGVGLLVDRGTAGGVPWHGSVLPYAVFVVP
                     AILASSAMMTGFGESSWPVFGAIKWQGTYHAMLATPMRTRDVLVGHVAMVGGQLAVGA
                     GAFVALAAPFGVWRSWWVLLTVPVATLTGLAFATIMTAVAARSETEGWFTAIFRVVAT
                     PLMLFSGTYFPIESLPAALLPVAWVTPLWHGVEACRALATGLIDVGPLLGHLAALVAF
                     TAVGVVWSARELERRLVR"
     gene            complement(2180..>3031)
                     /locus_tag="PLB86_12910"
     CDS             complement(2180..>3031)
                     /locus_tag="PLB86_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC66091.1"
                     /translation="VTVTAGESFGFLGPNGAGKSSTMRMIAAVSPVTSGTLRIFGLDP
                     ATRGPDIRAALGVVPQKDTLDEELTVEENLWIYGRYFGLSWAECRRRATELLEFAQLT
                     ERAGDRVEPLSGGMKRRLTIARSLINDPRILLLDEPTTGLDPQARHVLWDRLFRLKRQ
                     GVTLVVTTHYMDEAEQLCDRLVVMDRGQIVAEGSPRALIQTHATREVLELRFADDDNG
                     IHVPSLQGIGERVEVLPDRLLVYADDGDGALAAAHARGIRPLSALVRRSSLEDVFLHL
                     TGRTLVD"
BASE COUNT          558 a         1085 c         1017 g          371 t
ORIGIN      
        1 ccactgctct cgggtccgta gatctcgacg acccggccgc gcggcagacc gccgatcccg
       61 agggcgatat cgagggcgat cgacccggtg gggatcacct cgatgggcgg tcggacgtcg
      121 tcacccagac gcatcacggc acccttgcca tgggccttct cgatctgccc catgacactt
      181 gccagggcct tgcccttgtc ctgcgctgtg gccatgccac ccaccatcct tcgtctcgag
      241 agtcggccgt ccataggcga cccgcgcgac gggccggtca ctcccccggt cgatgtgtcg
      301 accgggcaag tccagcgccc gtgttgtcga gtcggacgtt aggcgaaccc accgacagac
      361 ctcagcccac ggcctacagc ctgtggacaa tgggacgacg gtgcggacac ctgtggacaa
      421 acattaccga acggatgttc gagtgccacg cgacacgccg ggccggacaa ccatccctca
      481 gcgcagtagc aggacgtcga cgcgacgagc ggcaccgacg acaccgaagg cgcccatgac
      541 gccgaggtag gcgacgtgcc acagcaggcc gacccccacc tcgcccaggc acagcgcccg
      601 gcacagggcg accccgtgat agaggggcgt cgcctcgacg agccattggg cccaccccgg
      661 ataggccgac aacgggaaga acgtcgccga gagcaggaac atcggttgga ccacgaggtt
      721 cacgtagtcg aagtcgaccc acgagcgcat gtaggtcgtc gcgaacatgc cgaccgcgga
      781 gaacgcgaac gccacgaaaa ccgctgcggg cagcgccaac agcccccacc aggacggcaa
      841 gacgccggcc aacgcggcga cgacgaggaa ccctgcggca tacgccccgc cgcgcagcaa
      901 cgaccaggtg acctctccca ccgcgacgtc gcgcggcccc atgggggtgg ccaggaccga
      961 gtcgtagagc ttcgcgtact tcaacttgaa gaagacgttg aaggtcgagt ccatgacgcc
     1021 cgcgttcatc gccgacgtcg cgagcatcgc gggggcgacg aacacggcat acgaaacgct
     1081 gtgacctgcg tcggtggtca ctgagccgac cagcgccccc aacccgatgc ccagggagaa
     1141 caggtagaac accggctcgg cgaacccggt ggcgaacgcc acccattggc gccggaagga
     1201 gaggatgttg cgttcgacca gggtgcgcgc cagacccgcg ccagccggcc gcggcagcgc
     1261 ccctccgacg gcccgaggca cgcgcctgag gcggctcatc ggaccaaccg ccgttccaac
     1321 tcacgggccg accacacgac gcccacggcg gtgaacgcca ccagcgcggc tagatgcccg
     1381 agcagcgggc ccacgtcgat cagcccggtc gccagtgctc ggcaggcctc gacgccatgc
     1441 cacagcggcg tcacccaggc caccggcagg agggcggcgg ggagcgactc gatggggaaa
     1501 taggtgcctg aaaacagcat caacggcgtt gcgacgaccc gaaagatcgc ggtgaaccag
     1561 ccttcggtct cactgcgggc cgccaccgcc gtcatgatgg tcgcgaaggc cagccccgtg
     1621 agggtcgcca ccggaacggt gagcagcacc caccaggacc gccacacacc gaacggtgcc
     1681 gcgagcgcaa cgaacgcacc cgcccccacc gccagttgcc cgcccaccat ggcgacgtgc
     1741 cccacgagga cgtcgcgggt tcgcatcggg gtcgccagca tcgcgtggta ggtgccctgc
     1801 cacttgatcg ccccgaacac cggccaactg gactccccga aaccggtcat catcgccgag
     1861 ctcgccagga tcgcggggac gacgaagacg gcatacggga ggacggagcc gtgccacggg
     1921 acccctccgg cagtgccgcg gtcgacgagc agccccacgc cccacccgac ggccagcagc
     1981 gagagcagcg gggtccccag ccggctgtag agcatcccgc gccagacccg ccgcatcacc
     2041 cgcaggtgaa actcgacgat cggcacccac cgccgggtgg cagtgaggat cgcgcgcggc
     2101 tccggcgcgg gggcggcata ccccctcttc ggtatgccgt gcggctgggt ccgctcgtcg
     2161 cgagcgggtg ggtggtccat cagtccacca gcgtccgacc ggtgaggtgc aggaagacgt
     2221 cctccagcga cgagcggcgc accagcgcgg acaacggacg gatcccgcgg gcgtgggccg
     2281 cagcgagggc gccgtcgccg tcgtccgcgt agacgagcag gcggtcgggc agtacctcga
     2341 cccgttcacc gatgccctgc agcgacggca cgtggatacc gttgtcgtca tcagcgaagc
     2401 gcaactcgag aacctcgcgg gtcgcgtggg tctggatgag cgcccggggt gagccctcgg
     2461 cgacgatctg gccgcggtcc atgacgacca agcggtcgca caactgctcg gcctcgtcca
     2521 tgtagtgcgt cgtgacgacg agcgtgacgc cctggcgctt gagccggaac agtcggtccc
     2581 agaggacgtg gcgggcctgc gggtccaaac cggtggtcgg ctcgtcgagc agcaggatcc
     2641 gcgggtcgtt gatgagcgac cgggcgatgg tgaggcgacg cttcatccca cccgagagag
     2701 gctcgacccg gtcgccggcc cgctcagtga gttgggcgaa ctccagcaac tccgttgcgc
     2761 ggcgccgaca ttcggcccac gagaggccga agtagcggcc gtagatccag aggttctcct
     2821 cgacggtcaa ctcttcgtcg agcgtgtcct tctgcgggac gacacccagc gcggctcgga
     2881 tgtccgggcc ccgggtcgcc gggtcgaggc cgaagatccg cagggtgccg ctggtcacgg
     2941 gtgacacggc cgcgatcatc cgcatcgtcg aggacttgcc cgcgccgttc ggcccgagga
     3001 agccgaagga ctcccccgcg gtcaccgtga c
//
LOCUS       DAORPW010000258         3014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_11303_length_3014_cov_653.765123, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000258 DAORPW010000000
VERSION     DAORPW010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11303_length_3014_cov_653.765123"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLB86_12915"
     CDS             <1..552
                     /locus_tag="PLB86_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC66092.1"
                     /translation="LGSHDTSRIRTLVGGDLALVRAAAGLLFTMPGTPMVTYGDEIGM
                     RGRFGEDGRRPMPWPAGDGTHGETWDEGLLAAYRDLAGLRRGSVALLRGGLRWVYAAG
                     DALVFLREAPGETALVHVARAAHDPVRIDVRDLLGISDSMVGFGGGGELEAFTLTLGA
                     TGPDVSVRIWATGTGGSNGWGGR"
     gene            556..1644
                     /locus_tag="PLB86_12920"
     CDS             556..1644
                     /locus_tag="PLB86_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRC66093.1"
                     /translation="MVRARQRDIAQAAGVSEATVSRVLNDHPGVSEATRQSVMVALDV
                     LGFERPTYLRRKSAGLVGLILPELTNPIFPAFAQLISTALARRGFIPILCTQTAGGVH
                     EDDYVQQLLDHGVAGMINISGLHADSDSKPGRYFRLRELGMPLVLVNGAIDGLDVPTI
                     ANDDVAAIEMAIAHLTELGHVRIGLAAGPDRFIPVIRKLWGFRGAMARHTSLSRAEID
                     DLIVHELFTVDGGAAAAARLLDRGVTAIAAASDLMALGAVRAVRARGLRVPQDVSVIG
                     YDDSPLIAFSDPPLTTIRQNVTAMSETAVNSLVEIIGGSDSRAKEFTFRPELVVRGST
                     APCVVQARERHTPRARGLRAAKVSDERP"
     gene            1705..2133
                     /locus_tag="PLB86_12925"
     CDS             1705..2133
                     /locus_tag="PLB86_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRC66094.1"
                     /translation="MKISDVLRNKGTEVVTIRPDETVSRLLEALAEHRVGALVVSPDG
                     AAVAGIVSERDVVRHLHQRGAAVLVIPVSEIMTADVTTCTADDSVESLAHTMTDRRIR
                     HVPVVAEGALVAIVSIGDIVKHRIDSLQSERDQLVEYIQQ"
     gene            2257..>3014
                     /locus_tag="PLB86_12930"
     CDS             2257..>3014
                     /locus_tag="PLB86_12930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRC66095.1"
                     /translation="MTAETEALSERLGPYRLIQRIGEGGMGVVHLALDPHGRAVAVKV
                     LRPHVAHDPEARARLEREVATLARIRHPRVAPIIDCDLSGDRPFVVTRYIAGDALDDR
                     VDTRGPLSAGDLLLLARGLHGALEAIHAVGVVHRDLKPGNVLLEDDGEPVVIDFGIAH
                     VAEDPRLTATGLVMGTPGYLSPEVIEGAAVTAATDWWGWAATLAFAATGRPPFGRGSM
                     NIVLTRVRAGNSDLEGVDARLEPLLSAALSPRPD"
BASE COUNT          415 a          993 c         1105 g          501 t
ORIGIN      
        1 ctcggctcgc acgacacgtc acgcatccgc actcttgtgg gcggcgatct ggcgctggtt
       61 cgggccgcgg cggggctgtt gttcaccatg cccggcacac cgatggtcac ctatggcgac
      121 gagatcggca tgcgcgggcg cttcggtgag gacggccgcc gcccgatgcc gtggccggcc
      181 ggggacggca cccacggtga gacgtgggac gaagggctgc tcgcggcata ccgggatctc
      241 gccggcctgc gccgcgggtc ggtcgccttg ctccggggcg gcctgcgctg ggtgtatgcc
      301 gccggcgacg ccctcgtctt cctgcgcgag gcgccggggg agacggcgct cgtgcacgtc
      361 gcgcgcgcgg cgcatgaccc ggtgcggatc gacgtgcggg acctgctggg gatctcggac
      421 agcatggtgg gattcggtgg cgggggggag ttggaagcgt ttacgctcac ccttggcgcc
      481 accgggcctg acgtgtcggt ccggatctgg gcgacgggga ccggggggtc gaacggctgg
      541 ggagggaggt aggcgatggt gcgcgcgcgg caacgggata ttgcccaggc tgcgggggtg
      601 tccgaggcaa cggtgagccg ggtgctcaac gatcatcccg gcgtgtccga ggccacccga
      661 cagtcggtca tggtcgcgct ggacgtgctc ggcttcgagc gaccgaccta cctgcggcgc
      721 aagagtgctg gcctcgtggg gctcatcctg ccggagctca ccaacccgat cttcccggcc
      781 ttcgcgcagc tcatctcgac agccttggcc cgtcgcggct tcatcccgat cctctgcacc
      841 cagacggccg gtggcgtcca cgaggatgac tacgtccagc agttgctcga ccatggggtc
      901 gccggaatga tcaacatctc cggcctgcac gccgactccg actccaaacc gggccgctac
      961 ttccgactgc gcgagttggg tatgccgctc gtcctggtca acggggcgat cgacgggctc
     1021 gatgtcccca ccatcgccaa cgatgatgtc gccgccatcg agatggcgat cgcccacctg
     1081 accgagctgg ggcacgtgcg gatcgggttg gcggccggcc ccgatcggtt catcccggtg
     1141 atccgcaagt tgtggggctt ccgaggggcg atggcgcggc atacctcgtt gagccgggcc
     1201 gagatcgacg acctcatcgt ccacgaactc ttcacggtcg acgggggagc ggcggctgcg
     1261 gcgcggttgt tggaccgtgg cgtcacggct atcgccgcgg cctcggacct catggcgttg
     1321 ggagcggtcc gcgctgtgcg agcccgtggg ctgcgggtgc cgcaggacgt ctcggtgatc
     1381 ggctacgacg actcaccact catcgcgttc agcgatccgc cgctgacgac gatccgccag
     1441 aacgtcacgg cgatgagcga aacggccgtc aactccttgg tcgagatcat tggggggtcg
     1501 gactcccggg ccaaggagtt caccttccgg cccgagttgg tggtgcgtgg ctcgacggcc
     1561 ccgtgcgtcg tgcaggcgcg cgaacggcat acccctcggg cccgggggct gcgcgctgcg
     1621 aaggtgagcg acgaacggcc ctgaacggga attcgcgtga tgctaacgct ggcctgagtc
     1681 gtcgcggggg tctacggtcc gggtatgaag atctccgacg tgctgcgtaa caaagggacc
     1741 gaggtcgtca cgatccggcc tgatgagacc gtctcccgcc tgctcgaagc cctcgccgag
     1801 catcgagtcg gcgcgctcgt cgtcagcccg gacggtgcgg ccgttgccgg gatcgtcagc
     1861 gagcgtgacg tcgttcgcca tctgcaccag cggggtgccg cggtcctggt catcccggtg
     1921 agcgagatca tgaccgccga cgtgaccacg tgcaccgcgg acgactcggt ggagtcgttg
     1981 gcccacacga tgactgatcg tcggatccgc cacgtgccgg tcgtcgccga gggtgccctg
     2041 gtggccatcg tcagcatcgg cgacatcgtc aagcaccgca tcgactcgct gcagtccgag
     2101 cgtgaccaac tcgtggagta catccagcag taagcgggcg gcatacgcgg atcgtgtggc
     2161 cgggactccc tgtggggacg ttggggccac ccgatctcgg gttggcgaca cgccgagcat
     2221 gcccacggta ggggtgccct cacctaccgt tgtcccatga cggccgagac cgaggcgctc
     2281 tccgagcgac tgggccccta ccgcctgatc caacggatcg gcgaaggggg gatgggcgtt
     2341 gtccatctgg ccctcgaccc gcatgggcgc gccgtcgccg tcaaggtgtt gcggccgcac
     2401 gtcgcacacg atccggaggc acgcgcgcgg ctcgagcgtg aggtcgcgac cctggcccgg
     2461 atccggcatc cgagggtcgc gccgatcatc gactgcgacc tgtcggggga ccggccgttc
     2521 gtcgtcacgc ggtatatcgc cggtgacgcc ctcgatgacc gggtcgacac tcgtgggccg
     2581 ctgtcggccg gcgatttgct gctcctggcg cgtgggttgc atggcgcgct ggaggcgatc
     2641 cacgccgtcg gggtggtgca ccgggatctc aagccgggca atgtcctgct ggaggacgac
     2701 ggggagccgg tcgtcatcga cttcggcatc gcccatgtgg ctgaggaccc tcggttgacc
     2761 gcgaccgggc tggtcatggg cacgccgggg tatctgtcgc ccgaggtcat cgagggggct
     2821 gctgtcaccg ctgccaccga ctggtggggg tgggccgcga cgttggcctt cgcggcgacc
     2881 gggcgcccgc cgttcggtcg gggctcgatg aacatcgtcc tcacgcgcgt ccgcgccggc
     2941 aacagtgacc ttgagggggt cgacgcgcgg ctcgagccgc tgctgtcggc ggcgttgtcg
     3001 ccgcgccccg acga
//
LOCUS       DAORPW010000259         3005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_11374_length_3005_cov_680.610508, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000259 DAORPW010000000
VERSION     DAORPW010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11374_length_3005_cov_680.610508"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2963)
                     /locus_tag="PLB86_12935"
     CDS             complement(<1..2963)
                     /locus_tag="PLB86_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRC66096.1"
                     /translation="MMAQSGAARGAPALLARAHHGSVSKEQRALIEEDLKAGRLPAVV
                     ATSSLELGIDMGAVDLVVQVESPPSVASGLQRVGRAGHQVGAVSRGVLFPKFRGDLVQ
                     TAVVVERMRTGSIESLRVPANPLDVLSQHIVAMCAMDDWSVEELEALVRRAAPFAGLS
                     RLVLESVLDMLSGRYPSDEFAELRPRIVWDRVAGTLHGRRGAQHLAVTSGGTIPDRGL
                     YAVFLASGDGPGRRVGELDEEMVYESRVGDTFTLGTSTWRIEDITHDRVLVTPAPGQP
                     GRLPFWKGDTLGRPVELGAAVGAFVREVGGLAPAAARKRVGAAGLDEWAADNLLGYLT
                     EQREAIPQLPTDRMIVVERFRDELGDWRVVVHSPFGSPVHAPWALAVSARMRERFGVD
                     VQAMHGDDGIVLRLPDLDLGGEVGVNADAGSATIGAELTQELLDLILLDPDEVAELVT
                     AQVGGSALFAARFRECAARALLLPRRRPDRRQALWQQRQRASQLLEVASRYPAFPIVL
                     EAVRECVQDVFDVPGLVGLMRAIRSREVGALAVQTRVPSPFARSLLFGYVAQYLYEGD
                     SPLAERRAAALALDPTLLAELLGTGDGLSLRDLLDPAAVATTEAELQRLVPERAARDA
                     EDLADLVRVLGPLGVEDLVARSVTSDARVVGAWLVELESARRVIRIRIGGQERWAAVE
                     DAGRLRDALGVSLPVGVPEVFLAPVADPLGDLVARFARSHGPFGVHAVAAWLGLGPAV
                     VRDALRRLVAAGRLVEGELRPQGSADGGLDYCDAGVLRTLRRRSLAALRAEVEPVGPV
                     DYARFLPTWQGVGGTLHGPEGLLRAVEQLSGALLPASAVESLVLPSRVAAYQPAWLDE
                     LLAAGEVLWQGHGALPGDDGWVSLHVADLAPTTLAPATALADLGDIHAAILECLGNGG
                     AYFFRTLVDAVSAAVGPSPDEAVRSALWELVWGGQISGDTLAPLRALLGGGRTAHRRR
                     ATPGPRGRYAARGTSL"
BASE COUNT          464 a         1107 c         1088 g          346 t
ORIGIN      
        1 agggaggtgc cgcgggcggc ataccggcca cgaggccctg gggtcgcgcg gcgccggtgg
       61 gcggtccgcc ccccgccgag gagggcacgc aagggggcga gggtgtcgcc gctgatctgc
      121 ccgccccaga cgagctccca gagggcactc cggacggcct cgtcggggct cggacccacg
      181 gccgcggaca ccgcgtcgac gagggtgcgg aagaagtagg ccccgccgtt gccgaggcat
      241 tcgaggatgg cggcgtgaat gtcgccgagg tcggcgagcg cggtcgctgg ggccaaggtc
      301 gtcggcgcca ggtcggcgac atgcagcgac acccacccgt catcgcccgg gagcgcgccg
      361 tgcccctgcc agagcacctc gccggccgcc agcaactcat cgagccaggc gggctggtat
      421 gccgcgacgc ggctgggcag caccaggctc tccacggcac tggctgggag aagtgcgccg
      481 ctgagctgtt cgacggcccg cagcagccct tcggggccgt gcagcgttcc gccgacgccc
      541 tgccaggtgg ggaggaagcg ggcgtagtcg accgggccga ccggctcgac ctcggcccgc
      601 aacgcggcca gggaccgtcg gcgcagtgtg cgcaggaccc cggcgtcgca gtagtcgagc
      661 ccgccgtcgg cgctgccctg gggccgcaac tcgccttcga cgaggcgccc ggcggcaacc
      721 aaccggcgca gtgcgtcgcg aacgaccgcc gggcccaggc ccagccaggc tgcgacggca
      781 tgcaccccga acggcccgtg gcttctagcg aatcgggcca ccaggtcgcc cagcgggtcg
      841 gcgaccggag cgaggaagac ctcaggcacg ccgactggca gcgacacccc gagggcatct
      901 cggagccgac cggcgtcctc gaccgcggcc catcgctcct gcccgccgat gcggatccgg
      961 atgacccgcc gcgccgactc gagctcgacg agccacgcgc cgacgacgcg ggcatctgag
     1021 gtgacgctgc gagcgaccag gtcctcgacc cccagaggcc ccaagacccg gaccaggtcg
     1081 gcgaggtcct cggcgtcgcg ggcggcccgc tcggggacca accgctgcaa ctcggcctcg
     1141 gtcgtggcca cagccgccgg gtcgagcagg tcacgcagcg acaggccgtc tcctgtgccg
     1201 agcaactcgg ccaggagcgt ggggtcgagc gcgagcgctg cggcccggcg ctcggccagc
     1261 ggtgagtcgc cttcgtagag gtattgagcg acatatccga acagcagcga gcgggcgaat
     1321 ggcgacggca ccctggtctg caccgccaac gccccgacct cgcgggagcg gatcgcccgc
     1381 atgaggccca ccagcccggg gacgtcaaac acgtcctgga cgcattcgcg gacggcctcc
     1441 aagacgatcg ggaaggcggg gtagcggctg gcgacctcga gcaactgcga ggcgcgttgg
     1501 cgttgctgcc agagggcctg acggcggtcg ggtcgacggc ggggtagcaa cagagcccgc
     1561 gccgcgcact cgcggaacct ggcggcgaac agcgcggaac cgccgacctg ggcggtgacg
     1621 agttcggcga cttcgtcggg gtcgagcagg atgaggtcga gcaactcctg cgtgagctcg
     1681 gcaccgatcg tcgcgctgcc ggcgtcggca ttgacaccaa cctcaccacc caggtcgagg
     1741 tcgggcagcc gcaggacgat cccgtcgtcg ccatgcattg cctgcacgtc gacgccgaac
     1801 cgctcccgca tccgtgccga cacggccaac gcccacgggg catggaccgg gctgccgaaa
     1861 ggggagtgga cgacaacgcg ccagtcgccc agctcgtcgc ggaatcgttc gacgacgatc
     1921 atccggtcgg tcggcagttg ggggatcgcc tcgcgctgct cggtgaggta gccgagcagg
     1981 ttgtcagcgg cccactcgtc caggccggcg gcgccgaccc gcttccgggc tgcagccggg
     2041 gccaacccgc cgacttcgcg cacgaacgca ccgaccgcgg ccccgagctc gactggccgc
     2101 ccgagggtgt cgcccttcca gaacggcagc ctgcccggct gacccggtgc cggcgtcacc
     2161 aggacgcggt cgtgggtgat gtcttcgatc cgccacgtcg acgtcccgag ggtgaacgtg
     2221 tcgccgaccc gcgactcgta gaccatctcc tcgtccagct ccccgacccg tcgtccggga
     2281 ccgtcgccag aggcgaggaa cacggcatac aggccgcggt cggggatggt gccgcccgag
     2341 gtgacggcga ggtgctgggc cccgcggcgc ccgtggagcg ttccggcgac gcggtcccag
     2401 acgatccggg ggcgcaactc agcgaactcg tcggagggat agcgcccgga caacatgtcg
     2461 agcaccgact ccagcaccag ccgcgacagc cccgcgaagg gtgccgcccg ccggaccagg
     2521 gcctccaact cctcgaccga ccagtcgtcc atggcgcaca tcgcgacgat gtgttgggag
     2581 agcacgtcga gcgggttggc ggggacgcgc agcgactcga tcgacccggt gcgcatccgc
     2641 tcgacgacga cggcggtctg cacgaggtcg ccgcggaact tcgggaacag cacgccccga
     2701 gacaccgccc ccacctggtg accggcccgg ccgacccgct gcaggccgct ggccacggag
     2761 gggggcgatt cgacctggac gaccaggtcg acggcgccca tgtcgatgcc caactcgagg
     2821 ctgctcgtgg cgacgaccgc gggcagccgt ccggccttga ggtcctcctc gatgagcgcc
     2881 cgctgctcct tgctcactga gccgtggtgg gcgcgagcga gcagcgcagg cgccccgcgc
     2941 gcggcccccg actgagccat gatctgcgcc ggcatcccac cggccgcacc tccccaggcc
     3001 gcacc
//
LOCUS       DAORPW010000260         2998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_11405_length_2998_cov_607.233435, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000260 DAORPW010000000
VERSION     DAORPW010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11405_length_2998_cov_607.233435"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..2108)
                     /locus_tag="PLB86_12940"
     CDS             complement(3..2108)
                     /locus_tag="PLB86_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-like protein EF-G2"
                     /protein_id="HRC66097.1"
                     /translation="MSKATAARELSSAPVPASPEQIRNVVLVGPGGAGKSTLFEHLVA
                     ARVPGRRPHEGEHRRSVGVEVAAVESGGLTVNLIDTPGYPDFVGELRAGLRAADAAIF
                     VISGADGIDGATALLWQECASVGMPRIIAVSKLDMPRSDYADTIATCRRVFGDAQPVY
                     LPVHTDDKTVVGSMALISQYVADGTGSRRDPSEDEAAAIEDQRGTLIEAIIEESEDDS
                     LLDRYLSGEDLDVAQLIADTKTAIGKGTFFPVLPISPATGVGMEEIFELIETAFPSPA
                     ARPSPAATTPAGGDVAGLACDPNGPLVAEVIRTSSDPYVGRLSLVRVFSGTLRAEEVV
                     HVSGHLDTFLGHELEGHPAHDDDERVGPLSSPLVDSLRTKEVAIAGDIALVAKLAKAE
                     TSDTLSAKDHPVLVEPWVLPEPLLPVAIHAKSKADEDKLASALQRLVAEDVTMRMEHN
                     AETHQVVLWAMGQAHIEELLGRLAERYGVQVEAEPFRTALRETFVKKVIVKGRHVKQS
                     GGHGQYAVCDLEIEPLPRGGGFEFVDKVVGGAVPRNFIPSVEKGARGQLDKGVLAGYP
                     LVDVRVTLFDGKAHSVDSSDMAFQTAAAMALKEAANESTVALLEPIDAVDITVSDDYV
                     GAVMSDLRGRRGQVLGTEPARESGWTIVHAEVPQTELSRYPIDLRSVSHGTGSFARRQ
                     LRYDYMPADKAKEYAKPEK"
     gene            2289..2417
                     /locus_tag="PLB86_12945"
     CDS             2289..2417
                     /locus_tag="PLB86_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66098.1"
                     /translation="MSALPRRRHGQQTDAQRGPQMLFIDLTRARDNDDADPDDGGE"
     gene            complement(2507..>2998)
                     /locus_tag="PLB86_12950"
     CDS             complement(2507..>2998)
                     /locus_tag="PLB86_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRC66099.1"
                     /translation="CGGIQVWSIYAPNGRHLTDPHMDYKMEWLGALRDEAAGWLAADP
                     AAQVVLMGDWNIAPRDEDVWSMEFYADKSHVSPRERAAFDAIVDAGFTDVVRPHTPGP
                     GVYTYWDYQQLRFPKKQGMRIDFVLGSPAFAARVDGAVIDREERKGTGASDHAPVVVQ
                     LTD"
BASE COUNT          452 a         1033 c         1063 g          450 t
ORIGIN      
        1 gcctacttct cgggcttggc gtactccttg gccttgtcgg ccggcatgta gtcgtagcgc
       61 aactgccgac gggcgaagga ccccgtgccg tgcgagaccg aacgcaggtc gatcgggtag
      121 cgcgacaact cagtctgggg cacctcggcg tggacgatcg tccagcccga ctcgcgggcc
      181 ggctcggtgc ccagcacctg gccgcggcga ccgcgcaggt cggacatgac cgcgcccacg
      241 tagtcgtccg acaccgtgat gtcgaccgcg tcgatcggct caagcagagc caccgtcgac
      301 tcgttggcgg cctccttgag ggccatcgcc gccgccgtct ggaaggccat gtcggatgag
      361 tcgaccgagt gcgccttgcc gtcgaagagc gtcacacgca cgtccaccaa cggataaccc
      421 gcgagcacgc ccttgtcgag ttgaccgcgg gcgcccttct ccaccgaggg gatgaagttg
      481 cgcggcacgg cgccgccgac gaccttgtcg acgaactcga agccaccacc gcgggggagc
      541 ggctcgatct ccaggtcgca cacggcatac tgcccatgcc cgccggactg cttgacgtgc
      601 cggcccttga cgatgacctt cttgacgaac gtctcgcgca gcgcggtccg gaacggctcg
      661 gcctcgacct gcacgccata gcgctcggcc agccgcccga gcaactcctc gatgtgcgcc
      721 tggcccatcg cccagaggac gacctggtgg gtctcggcgt tgtgctccat ccgcatcgtc
      781 acgtcttcgg cgacgaggcg ctgcaaggcc gacgcgagct tgtcctcgtc ggccttggac
      841 ttggcgtgga tcgcgaccgg cagcagtggc tcgggcagca cccacggctc gacgagcacc
      901 gggtggtcct tggccgacaa ggtgtcggag gtctcggcct tggcgagctt ggcaaccagc
      961 gcgatgtcac cggcgatggc cacctccttg gtgcgcaacg aatccaccag cggcgacgac
     1021 aacgggccga cgcgctcatc gtcgtcgtgg gccgggtggc cttcgagctc atggccgagg
     1081 aaggtgtcga ggtgacccga gacgtggacg acctcctcgg cgcgcagggt gccggagaac
     1141 acccggacca gggagagccg cccgacatag gggtcactgc tcgtgcgaat gacttcggcg
     1201 acgagcggac cgttcgggtc gcacgccagc ccggcgacat cgccgccggc gggagtggtc
     1261 gctgccggag acgggcgcgc agccggcgac gggaacgccg tctcgatgag ctcgaagatc
     1321 tcttccatgc cgacaccggt cgccggcgag atgggcagga cggggaagaa ggtgcccttg
     1381 ccgatcgccg tcttggtgtc ggcgatgagc tgggcgacgt cgaggtcctc gccggacagg
     1441 tagcggtcca gcagcgagtc gtcctcggac tcctcgatga tcgcctcgat gagcgtgccg
     1501 cgctggtcct caatggcggc ggcttcgtcc tcggagggat cgcggcggct gcccgtgccg
     1561 tcggcgacgt actggctgat gagggccatc gacccgacga ccgtcttgtc gtcggtgtgg
     1621 accggcaggt agaccggctg ggcgtcgccg aagacccggc ggcaggtggc gatggtgtcg
     1681 gcatagtccg accgcggcat gtccagcttg ctcacggcga tgatgcgcgg cataccgacg
     1741 gatgcgcact cctgccacag cagggcggtc gcgccgtcga tgccgtccgc gccggagatg
     1801 acgaagatgg ccgcgtcggc ggcgcgcaac cccgcgcgca gctcgccgac gaagtcgggg
     1861 tatccggggg tgtcgatgag gttgaccgtc agcccgccgc tctcgacggc ggcaacctcg
     1921 acgcccacgg agcgtcggtg ctcgccctcg tggggccgtc gacccggcac gcgcgccgcg
     1981 accaggtgct cgaagagggt ggatttgccc gcaccgccgg ggccgacgag cacgacattg
     2041 cggatctgct cggggctcgc ggggaccggt gcggacgaca gttctctggc ggcggttgct
     2101 ttgctcacgg gctccacctt tctctgtgcc gacccacccc gcaaggggac cgagggcccg
     2161 cctcagcgcg cctggccgga tgcttcccga cagcgtatgc cgcccgcccg gcatccggcg
     2221 gagcggggcc cgaacggggg cggtgagctt gctggaaccc tggcgtgaga ccaaccccga
     2281 ctgtcaccat gagcgcattg ccccgccgac ggcacgggca gcagaccgac gctcagagag
     2341 gaccgcagat gctgttcatc gacctcaccc gcgcccgtga caatgacgac gccgaccccg
     2401 acgacggcgg cgagtgacca caccggtctg acatcgcgcc caccgctgcg gcgggcgcga
     2461 cgcggacggt gagcgggcgg catacccctg aggtatgccg cccgcctcag tcggtgagtt
     2521 ggacgacgac cggcgcgtgg tcggaggccc cggtgccctt gcgctcctcg cggtcgatga
     2581 ccgcgccgtc cacgcgggcc gcgaaggccg gcgagccgag gacgaagtcg atgcgcatgc
     2641 cctgcttctt ggggaagcgc agttgctggt agtcccagta ggtgtagacg ccgggcccgg
     2701 gtgtgtgcgg ccgcacgacg tcggtgaagc ccgcgtcgac gatggcgtcg aaggccgccc
     2761 gctcacgcgg cgacacgtgc gacttgtcgg cgtagaactc catgctccac acgtcttcgt
     2821 cgcgcggcgc gatgttccag tcgcccatga ggacgacctg ggcggcgggg tcggccgcaa
     2881 gccaaccggc ggcctcgtcg cgcagcgcgc cgagccattc catcttgtag tccatgtgcg
     2941 ggtcggtcag gtgccgtccg ttgggggcat agatcgacca cacctgtatg ccgccgca
//
LOCUS       DAORPW010000261         2983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_11496_length_2983_cov_813.603484, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000261 DAORPW010000000
VERSION     DAORPW010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11496_length_2983_cov_813.603484"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PLB86_12955"
     CDS             complement(<1..161)
                     /locus_tag="PLB86_12955"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRC66100.1"
                     /translation="MTAFPGLTAGTWTVDPTHAEVGFVARHLMVAKVRGRFTEVSGTV
                     EVGETLAETS"
     gene            404..904
                     /locus_tag="PLB86_12960"
     CDS             404..904
                     /locus_tag="PLB86_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC66101.1"
                     /translation="MAAHSETNWLTEPEQRAWRAYLRGGRMLIEALDRGLAAHGLSLS
                     EYEIISMLSEEDTRQMRMSELADLVVQSRSRLTHTATRLEKRGWVVREPCQGDRRGVL
                     LTLTPTGAAKVEQIAPVHLDQVRAHLVDRLSAEQFATLGGAMENVMSGLDGFADGDGR
                     APNDRY"
     gene            951..1598
                     /locus_tag="PLB86_12965"
     CDS             951..1598
                     /locus_tag="PLB86_12965"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRC66102.1"
                     /translation="MSTPESTVVHLMRHGEVHNPDGILYGRLPGFRLSELGRAMATEV
                     AQHLAAHDIVHVVSSPLERALETARPTADSHGLGIHVDGRLIEAGNFFEGKVVGVGDG
                     VLKHPEYWSKLRDPFTPSWGEAYAAIASRMIAAVADARDAARGHEALLVSHQLPVWTA
                     RSRLEGRRLWHDPRKRQCALASLTSLHYLGNELVSVVYSEPAATLVRDAAKKAGA"
     gene            1595..2203
                     /locus_tag="PLB86_12970"
     CDS             1595..2203
                     /locus_tag="PLB86_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRC66103.1"
                     /translation="MTSTPVRWVAAGLALGAALSLAACQSDPNSLAAQAGSGSRAGYA
                     AGDGSIEQLPPASRGQALALSGTTVDGGTWSMDVQGAGKVVVINVWGSWCPPCVAETP
                     ALQGAWAAYEKAGKPVVFVGIDTMEAPETGLAFLRAKGVTFPSINDQANQGAPVLALQ
                     GKAPATPTTLVLDRQGRVAARVLGPTSEVTVRALVDAVLAEA"
     gene            2200..2973
                     /locus_tag="PLB86_12975"
     CDS             2200..2973
                     /locus_tag="PLB86_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HRC66104.1"
                     /translation="MTSLVLTDTSTIISGALPLAVLVAVAAGLVSFASPCVLPLVPGF
                     LGYVTGLSDVALADRRRSRMILGAALFVAGFSVVFIIGALAVSAAGAALAAHQSLLTR
                     LGGAVVIVLALVFLGVGAGMGSQREMRAHWRPAAGLAGAPLLGAAFAVGWSPCTGPTL
                     GAIYSLAAPLAGDAPVARGALLAAAYSVGLGVPFVLAAAGWSRAARANDWLRRHQRGV
                     QLAGGGLLLALGLLMVSGAWEGIMSWVQSHLIIGFETVL"
BASE COUNT          397 a         1034 c         1068 g          484 t
ORIGIN      
        1 gaggtctcgg cgagggtctc gccgacctcg acggtgccac tgacctcagt gaagcggccg
       61 cgaaccttgg ccaccatgag gtggcgggcg acgaaaccca cctccgcgtg ggtggggtcg
      121 acggtccacg tgccggcggt cagtccgggg aaagcagtca tgatggtcct ttcgggtggg
      181 ggccgtcggg cggccgttga taaggcaaat aagattgaac tctcaactat tcccagaagc
      241 ctgcgatgtc tcaccatctg gacctgacct ggtcggaccc cgcggagtga cgtgcacggc
      301 gtctgtccga cagctagccc gcgggcccta tgctgtgcat acggcgggcc ggggtcgcga
      361 ctcggggccg ctgcagggat cccgtcgata tgaatgcgca ctgatggccg ctcacagcga
      421 aaccaactgg ctcaccgagc ccgaacaacg ggcctggcga gcctacttgc gcggcggtcg
      481 catgctcatc gaagccctcg accgggggct ggcggcgcac gggctgagcc tgtcggaata
      541 cgaaatcatc tcgatgctgt ccgaggaaga cacccggcag atgcggatgt ctgagctcgc
      601 cgacctggtc gtccagtccc gcagccgact cacccacacc gcgacccggt tggagaagcg
      661 gggctgggtc gtgcgcgagc cgtgccaggg tgaccgacgg ggagtgctgc tcacgctcac
      721 gcccaccggt gccgccaagg tggagcagat cgctcccgtc cacctcgacc aggttcgagc
      781 ccacctggtc gaccgattga gcgccgagca gttcgcgacg ttgggcggag cgatggagaa
      841 cgtcatgtct ggcctggacg ggtttgcgga cggcgacggc cgggcgccca acgaccgcta
      901 ctgattggtg ggccgctgcg gcccacagtg agtgctggaa gaatggggcc atgtccactc
      961 cggagtcgac cgttgtccac ctcatgcgcc acggcgaggt tcacaacccc gacgggattc
     1021 tttacggccg gctgcccggc ttccggctgt ccgagttggg tcgggccatg gcgacggagg
     1081 ttgcgcaaca cctggccgcc cacgacatcg tccacgtcgt gtcctcgccg ttggagcgcg
     1141 cgctcgagac cgcccgaccg acggccgaca gccatggcct gggcatccac gtcgacggtc
     1201 gcctgatcga ggctggcaac ttcttcgagg gcaaggtcgt cggggtcggt gacggcgtgc
     1261 tcaagcaccc ggagtactgg tccaagctgc gcgacccctt cacccccagt tggggcgagg
     1321 cgtatgccgc gattgcttcc cggatgatcg ctgcggtcgc cgacgcccgt gacgccgccc
     1381 gtgggcacga agcgctgctc gtcagccacc agcttcccgt gtggacggct cgctcccgcc
     1441 tggaagggcg ccggctatgg cacgacccgc gcaagcgcca gtgcgccctc gcctcgctga
     1501 cgtcgctgca ctacctcggc aacgagctcg tctcggtcgt ctacagcgag cccgccgcca
     1561 ccctggtgcg cgatgctgcg aagaaggctg gcgcgtgacc tcgacgccgg ttcggtgggt
     1621 cgctgcgggg cttgcgttgg gggccgccct ctcgctggcg gcctgccaga gcgaccccaa
     1681 ctccctcgcc gcccaggctg gttcgggcag ccgcgcgggg tatgccgcgg gcgacggctc
     1741 gatcgagcaa ctgcccccgg cctcgcgcgg gcaggctctc gcgctctccg ggacgactgt
     1801 cgacggaggc acctggtcga tggacgtgca gggcgctggc aaggtcgtcg tcatcaacgt
     1861 ctgggggtcc tggtgcccac cgtgcgtggc cgagacgccg gccctgcaag gtgcgtgggc
     1921 ggcatacgag aaggccggta agccggtcgt cttcgtggga atcgacacga tggaggcccc
     1981 agagacgggg ctggccttcc tgcgggccaa gggcgtgacc ttcccgtcga tcaacgacca
     2041 ggccaaccag ggtgcccccg tcctcgcgtt gcagggcaag gcgcctgcca cgccgacgac
     2101 cctcgtgctc gaccgtcagg ggcgggtggc cgcccgggtg ctcggcccga ccagcgaggt
     2161 caccgtccgg gcgctcgtcg acgccgtgct tgccgaggcg tgacctcgct cgtgctcacg
     2221 gacacctcga cgatcatctc cggcgcgctt ccgctggcgg tgctcgtcgc ggtggcggcc
     2281 gggctggtgt ccttcgcctc gccgtgcgtg ctgccgctgg tgccggggtt cctcggctat
     2341 gtcaccggcc tgtccgacgt ggccctggcc gaccggcggc gctcgcggat gatcctcggg
     2401 gctgccttgt tcgtcgcggg cttcagcgtc gtcttcatca tcggagccct ggccgtgtcg
     2461 gccgcggggg cggccctcgc ggcgcaccag agcctgctga cccgcctcgg cggggccgtc
     2521 gtcatcgtcc tggcgctggt cttcctcggc gtcggtgccg gcatgggcag ccaacgcgag
     2581 atgcgcgcgc actggcgccc ggcggccgga ctggcggggg cgccgttgct cggcgccgcg
     2641 ttcgccgtcg gctggagccc ctgcaccggc cccaccctcg gggcgatcta ctccctcgcg
     2701 gcgcccctgg ccggtgacgc cccagtcgcg cgcggagccc tgctggcggc ggcatacagc
     2761 gtgggtttgg gggtgccctt cgtcctggct gccgcggggt ggtcacgcgc agcacgggcc
     2821 aacgactggc tgcgccgcca ccagcgcggc gtgcaactgg ccggcggcgg gctgctgctc
     2881 gcgctgggcc tgttgatggt cagtggcgcc tgggaaggca tcatgtcgtg ggtgcagtcc
     2941 cacctgatca tcggattcga gaccgtgctg tgagcgctga cga
//
LOCUS       DAORPW010000262         2944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_11757_length_2944_cov_581.178262, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000262 DAORPW010000000
VERSION     DAORPW010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11757_length_2944_cov_581.178262"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLB86_12980"
     CDS             <1..526
                     /locus_tag="PLB86_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRC66105.1"
                     /translation="AGWSACYRREAGSHGKDTRGIIRVHQFHKVEMFVYCRPEDAEAE
                     HERLLGMERAMLDACELPYRIIDTAAGDLGDPAARKFDCEGWVPTQGRYRELTSTSNC
                     TTFQARRLGVRERDPQASGGATRIVATLNGTLATTRWIVAILENHQQADGSVVVPEAL
                     RPFMGGLAVLEPVR"
     gene            595..1374
                     /locus_tag="PLB86_12985"
     CDS             595..1374
                     /locus_tag="PLB86_12985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRC66106.1"
                     /translation="MIALDVDGTIITHAGHLDDRVRDAIEGCVHDYQHVVIATGRSVV
                     ATMPVLARLGITQGYAVCSNGAVTLRIDPSRPKGYKILEKVTFDPRPALTLLREELPE
                     ALVAVEDLGRGFLVSSPFPDGELMGDQRVVPWDKLVGRRATRVTLRMPDAEPEAFMAH
                     IERIGLHEVSYAVGWTAWLDINPEGVSKGSALELLRRRLGVEPCDTVAVGDQRNDIEM
                     LQWAARGVAMGNAPDEVKAVADEVTGSVDEAGLAAVLESLR"
     gene            1374..2198
                     /locus_tag="PLB86_12990"
     CDS             1374..2198
                     /locus_tag="PLB86_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRC66107.1"
                     /translation="MDLHLTSPANPRLKALVGLRRRRTRDEAGLTVVEGHEELSLALA
                     AGVAPQTLYVCSELFSPSGIAGSQLIGSQPEFVSAARAAGSEIVTLSRVAFEKVAYRE
                     GPDGLLGVLPAVDRPLSGLTLPVDPLVLLVEGVEKPGNLGAMLRTADAAGVDAVVAAD
                     PVTDWGNPNVVRASKGTVYAVPVASASTAETLAWCRARGIRLVVTTPETDVLYSDISL
                     SGAVAIAVGAEKHGASEELLAAADVRVRIPMSGRANSLNVAASAAIVLFEAVRQRR"
     gene            2281..2916
                     /locus_tag="PLB86_12995"
     CDS             2281..2916
                     /locus_tag="PLB86_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC66108.1"
                     /translation="MRVDHVSYAAEHDGLHATAERLAKLIGVKPVDGGVHPRFGTRNV
                     ILPLANERYVEVVEVLDHPASDKAPFGQAVRARSANGGGWLGWVVRVDGIEEIGSRLG
                     REPADGYRHTPDGRELRWRQLGVSGLICDPQLPFYVEWAEGTRHPSTDGATQVGLAGL
                     TIAGDPQRVRAWLGLPEDYKPSTLEFTFVAPHGNPGLLAVTFGTPDGPVTV"
BASE COUNT          412 a          984 c         1064 g          484 t
ORIGIN      
        1 tgccgggtgg tcggcctgct accgccgcga ggccggcagc cacggcaagg acacccgcgg
       61 gatcatccgg gtccaccagt tccacaaggt cgagatgttc gtctactgcc gtcccgagga
      121 cgccgaggcc gagcacgagc gcctgctggg catggagcgg gcgatgctcg acgcctgcga
      181 gctgccctac cggatcatcg acaccgccgc aggcgacctc ggcgacccgg ccgcccgcaa
      241 gttcgactgc gagggctggg tgcccaccca gggccgctat cgcgagctga cctcgacgtc
      301 caactgcacc accttccagg cccgtcgtct cggggtccgc gagcgcgacc cgcaggccag
      361 cggcggggcg acccggatcg tcgccaccct caacgggacc ctggcaacga cccggtggat
      421 cgtcgcgatc ctcgagaacc accagcaggc cgacggctcg gtcgtcgtgc ccgaggcgtt
      481 gcggcccttc atgggcgggt tggccgtcct cgagccggtc cggtgaccaa gggagccaag
      541 gggagccggg gccgcggcgg caagcccgcg gcatacccgg ggtttcgtcg tcgcctgatc
      601 gcgctcgacg tcgacgggac gatcatcacc cacgccggcc acctggacga tcgggtgagg
      661 gacgcgatcg aggggtgtgt gcacgactac cagcacgtcg tcatcgcgac cggtcgctcg
      721 gtcgtcgcga cgatgccggt cctcgcgcgg ctggggatca cccaggggta tgccgtgtgc
      781 tccaacggtg cggtgacgct gcggatcgac ccgtcccggc ccaaggggta caagatcctc
      841 gagaaggtca ctttcgaccc gcggccggcg ctgacgttgc tgcgggagga gttgcccgag
      901 gcgctcgtgg cggtcgaaga cctggggcgc ggcttcctcg tcagttcccc tttccccgat
      961 ggcgagctca tgggcgacca gcgggtcgtg ccgtgggaca aactcgtggg gcggcgcgcc
     1021 acgcgtgtca cgctgcggat gcccgacgcc gagcccgagg cgttcatggc gcacatcgag
     1081 cggatcggcc ttcacgaggt ctcgtatgcc gtcggctgga ccgcctggct ggacatcaac
     1141 cccgaggggg tctccaaggg gtcggcgctg gagttgttgc ggcgccggct gggcgtcgag
     1201 ccgtgcgaca ccgtcgccgt tggcgaccag cgcaacgaca tcgagatgct ccagtgggct
     1261 gctcgtgggg tcgccatggg caacgcgcct gatgaggtca aggcagtcgc tgacgaggtg
     1321 accggctcgg ttgacgaggc cggcctggct gcggtgctcg agtcgctgcg ctgatggacc
     1381 tgcatctgac ctcccctgcc aacccgcgcc tcaaggcgct ggtggggttg cggcgtcgac
     1441 gcacgcgcga cgaggctggg ctgacggtcg tcgagggcca cgaggagttg tcgttggcgt
     1501 tggcggccgg ggtcgcgccg cagacgctct atgtctgctc agagttgttc agccccagcg
     1561 gaattgccgg gtcacagctg atcgggtcgc agcctgagtt cgtctcggcg gcccgggcgg
     1621 cggggtcgga gatcgtcacc ttgtcgcggg tggccttcga gaaggtcgcc tatcgcgaag
     1681 ggcccgatgg gctcctcggg gtgcttccgg cggtagatcg gccgctctcg ggtttgaccc
     1741 ttcccgttga cccgctggtg ctgctcgtcg agggtgtcga gaagcctggg aacctcgggg
     1801 cgatgctccg caccgccgat gcggccgggg tcgacgccgt tgtggcggcg gatccggtga
     1861 ccgactgggg caaccccaac gtcgtgcggg ccagcaaggg caccgtgtat gccgtgcccg
     1921 tcgcctccgc gtcgacggcc gagacgttgg cgtggtgcag ggcgcgcggc atacgcctcg
     1981 tggtgacaac ccctgagacg gatgtcctct attcggacat ctctttgagc ggggcggtcg
     2041 cgatcgcggt cggtgcggag aagcacggcg catccgaaga attgctcgcg gcggcggacg
     2101 ttcgcgtgcg aattcccatg tctggcaggg ccaattcgtt gaacgttgca gcgtcagcgg
     2161 cgatcgttct gttcgaagca gtgcggcagc gaaggtagta gcgcttacat tgcggtggcc
     2221 cccggcgccg gggccgacag tggtcaaaag caccctcaaa cctgtgtcac gatgggcggc
     2281 atgcgcgttg accacgtctc ctacgcggcg gagcacgacg gccttcatgc caccgctgag
     2341 cggttggcca agctgatcgg agtgaagccg gtcgacgggg gcgtccaccc tcggttcggc
     2401 actcgcaacg tcatcctgcc cctcgccaac gaacgctacg tcgaggtcgt cgaggtgctt
     2461 gaccaccccg cttccgacaa ggcccccttc ggtcaggcgg tccgcgcccg gtccgccaac
     2521 ggtggcggct ggctcggctg ggtggtccgg gtcgatggca tcgaggagat cggcagccgg
     2581 ttgggtcgcg agcccgccga cggataccgg catacccctg acggtcgtga gttgcgctgg
     2641 cgccagctgg gcgtgagcgg gctcatctgc gacccacagc tccccttcta cgtcgagtgg
     2701 gccgaaggca cccgccaccc gtcgaccgat ggcgccacgc aggtcgggtt ggctgggctg
     2761 accatcgccg gcgacccgca gcgggtccgg gcctggctgg ggcttcccga ggactacaag
     2821 ccgtcgacgt tggagttcac tttcgttgcg ccgcacggca acccgggtct tctcgcggtc
     2881 acgttcggca cgcccgacgg ccccgtcacc gtctgagccc cgcctctttg gtgggtttgc
     2941 cggc
//
LOCUS       DAORPW010000263         2942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_11768_length_2942_cov_704.746450, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000263 DAORPW010000000
VERSION     DAORPW010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11768_length_2942_cov_704.746450"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /gene="rplT"
                     /locus_tag="PLB86_13000"
     CDS             <1..237
                     /gene="rplT"
                     /locus_tag="PLB86_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRC66109.1"
                     /translation="RAKKGDFRRLWIQRINAAARANGMTYNRFMQGLKAAEVEVDRKI
                     LADLAVNDAAAFTALVELARKAVPAQGTSAGDAA"
     gene            298..1096
                     /locus_tag="PLB86_13005"
                     /pseudo
     CDS             298..1096
                     /locus_tag="PLB86_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775192.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
     assembly_gap    1103..1202
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1247..2401
                     /locus_tag="PLB86_13010"
     CDS             1247..2401
                     /locus_tag="PLB86_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC66110.1"
                     /translation="MNVADGVALAGVHQTPADVVRAISSTIPDGLLVLDGSRRLVFVN
                     RRAEEILQSPISGSLGLSIEEAVPLWDSEGRLWWDVANPWRQLHTVKGHREKLLWTGS
                     GVEVLATARYLRPEPRQPVERVVVALRDALARQRAERDHAALISTLAHELRSPLTSVK
                     GFSSTLLRRWDRFTDEQKRLIIETIEADADRVTRLVSDLLDVSRIEAGRMQVRRREIF
                     LDPLIARHLRRLTGLGIPESRIEVEVNRPIPDCWADPDRIDQILINLVDNALTHGAGQ
                     IRVRVTSGTVDMVDGADGDARPACIVSVADEGQGIPERLRAVIFTRFWHGPGQRNTGL
                     GLYIVKALVEAHGGRVRATNGPAGGAVFTFSIPAGDPALALSEAAALSVG"
     gene            2518..>2942
                     /locus_tag="PLB86_13015"
     CDS             2518..>2942
                     /locus_tag="PLB86_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRC66111.1"
                     /translation="MSGPTAPLDPAPLEPAALDAALDAAREAFAAAGDLDALKAARLA
                     HVDKGAIAAARREIGALPPAAKAEAGKLVGAALDAVRAAYTSREEALQAVRDERILVE
                     ERVDVTMPTARRPLGARHPIELTQERLADIFIGMGWEVA"
BASE COUNT          402 a         1024 c          988 g          428 t
ORIGIN      
        1 cgggccaaga agggtgactt ccgtcgcctg tggatccagc ggatcaacgc tgcggcccgc
       61 gccaacggca tgacctacaa ccggttcatg cagggtctca aggccgccga ggtcgaggtc
      121 gaccgcaaga tcctcgccga cctcgccgtc aacgacgccg cggctttcac cgcgctggtc
      181 gagttggctc gcaaggcggt gcccgcgcag ggcacgtccg ccggcgacgc agcctgagcc
      241 acacggcata ccgctgcacg acactcaccg catgacgctg agcgccggga cacccgcgtg
      301 ctgaccaatc cccgagccga tcgggtgcgg tcggtcgcgg cgctgtcccg gcgctcagcg
      361 cgtgagcggg cggggttgtt cgtcgccgag gggccgcagt cggtccgcga ggcggtccgg
      421 ttccggcccg aggcgatccg cgacctctac gtcacggcgg aggcggggga gcggcatacc
      481 gagatcgtcg acgcggccac cgcagcgggc gtccacgtgc acgagtgcac ccccgaggtc
      541 ctcgccgcga tgtccgacac cgacgcgccg caggggctgc tcgcggtatg ccgcgtgctc
      601 acctccaccc tcgcggacgt cctcgcgcgc cgtcccccgg ctgctcgtcg tcctggccca
      661 tgtccgcgat cccggcaacg cgggcacggt gctgcgcggg gccgatgccg cgggagccga
      721 tgctgtcatc gtcagcgagg cgagcgtcga cgtcttcagc cccaaggtgg tccggtcaac
      781 cgccgggtcg ctcttccacg tgcccgtggt caccggtctg ccggtcccgc agatcctcgc
      841 cgagttggcc ggggcgggga tcacccggct ggccgccgac ggagccggaa cccgcctcct
      901 gcccgaggtc gacctgaccc ggccccacgc ctgggtgatg gggaacgagg cgtgggggct
      961 gccgcagccg ctgcgcgagg cctgcgacga ggtcgtccgg gtgccgatct tcggtcatgc
     1021 cgagtcgctc aatctggcga tggcggccac cgtctgcctt tatgcctcgg cgttggcgca
     1081 acatccagca gggtgacccg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nngcaggatc gtcgcggttg actgcggcga tgactaatct gacgacatga acgtcgcgga
     1261 tggggtggcg cttgcgggcg ttcaccaaac tcccgcagac gtggttcggg ccatctcgtc
     1321 gacgattccc gatggcctcc tggtgctgga cggttcgcgc cggctcgtgt tcgtcaaccg
     1381 ccgggccgag gagatcctcc agtcgccgat cagcgggtcc ttgggcctgt ccatcgagga
     1441 ggccgtgccg ctctgggact ccgaggggcg gctgtggtgg gacgtcgcca atccctggcg
     1501 acagttgcac accgtcaagg gccatcggga gaagctgctg tggaccggct caggggtcga
     1561 ggtgctcgcc acggcacgtt acctgcggcc cgagccgcgc caaccggtcg aacgagtcgt
     1621 cgtcgccttg cgcgatgccc tcgcccgcca gcgcgccgag cgcgaccacg cagcgctcat
     1681 ttccaccctt gcccacgagt tgcgctcacc gctcacctcg gtcaagggct tctcctcgac
     1741 gctgcttcgt cgttgggacc gattcaccga tgagcagaaa cggctcatca tcgagaccat
     1801 cgaggctgat gccgaccggg tgacccggct ggtgtccgat ctgttggatg tctcgcggat
     1861 cgaagcgggc cggatgcagg tgcggcgacg ggagatcttc ctggatcccc tcatcgcccg
     1921 gcatctgcgc aggctcacgg gcctcggcat cccggagagc cgcatcgagg tcgaggtcaa
     1981 ccgccccatc ccggactgct gggccgatcc cgaccggatc gaccagatct tgatcaacct
     2041 cgtcgacaac gccctgaccc acggcgccgg gcagatccgg gtgcgggtga ccagcgggac
     2101 ggttgacatg gtcgacggcg ccgacggcga cgctcgccct gcgtgcatcg tctccgtcgc
     2161 cgatgaggga caaggcatcc cggagcgact ccgagcggtc atcttcacgc ggttctggca
     2221 cgggccgggc cagcgcaaca ccggcctggg gctctatatc gtcaaggcgc tggtcgaggc
     2281 ccacggcggg cgggtccgag ccaccaacgg cccggctgga ggggcagtct tcacgttcag
     2341 catccccgcc ggtgaccccg ccctcgcgtt gtccgaggct gcggcactga gcgtcggctg
     2401 agcggttccc gccccgggga cggtccaggc catgaactcg atgtcgaggc caccctcgcg
     2461 cggcctagac tcgggcgccg aacccgaacg ccgcgaccag cccgaatgtg gaacgtgatg
     2521 tcaggaccaa ctgctccgct cgaccctgct ccgctcgagc ctgccgcgct tgacgcggcc
     2581 ctcgatgcag cccgcgaggc gttcgccgct gctggcgacc tcgatgctct caaggccgcc
     2641 cggctcgctc acgtcgacaa gggcgccatc gccgcggccc gccgcgagat cggtgccctt
     2701 ccgccggccg ccaaggcgga ggccggcaag ttggtgggcg ccgcgctcga cgccgtgcgc
     2761 gcggcataca cctcgcgcga ggaggccctg caggcggtgc gcgacgagcg gatcctcgtg
     2821 gaggagcgcg tcgacgtcac catgccgacc gcgcggcggc cgctgggtgc gcgccacccg
     2881 atcgagctca cccaggagcg gctggccgac atcttcatcg gcatgggctg ggaggtcgcc
     2941 ga
//
LOCUS       DAORPW010000264         2923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_11893_length_2923_cov_730.633194, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000264 DAORPW010000000
VERSION     DAORPW010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11893_length_2923_cov_730.633194"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLB86_13020"
     CDS             complement(<1..476)
                     /locus_tag="PLB86_13020"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC66112.1"
                     /translation="MTSFESINVGDQGHVAHQYATTERLRGRMSVWGPGATGLDPTDV
                     LFEELVARSPRTVLEIGCGTGAFAKRVVDALPDVDYLATDASPAMVAAASAQGVRAEV
                     RPAEATGQPTGSIDVVVAAWMLYHVPSLDAALAEVCRVLRPGGALLAVTNGEQHLAT"
     gene            complement(696..>2923)
                     /gene="aceE"
                     /locus_tag="PLB86_13025"
     CDS             complement(696..>2923)
                     /gene="aceE"
                     /locus_tag="PLB86_13025"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HRC66113.1"
                     /translation="VDGKIRALSSYPHPRLMPNFWQFPTVSMGIGPMNAIYQAQFNRY
                     LLNRGIKDTSDQHVWAFLGDGEMDEPESRGLLQLAANEELDNLTFVVNCNLQRLDGPV
                     RGNGKIIQELEAFFRGAGWNVIKVVWGRGWDPLLAADRDGALANLMNQTSDGDYQTFR
                     ANDGKYVREHFFWRDPRTRKMVENWTDDEVWFKLRRGGHDYRKVYAAYRAALEHTGQP
                     TVILVKTIKGFTLGSHFAGRNATHQMKKLTLEDLKGFRDLLRIPITDEQLEADPYRPP
                     YYRPDASDPAMQYMRERRRVLGGSVPSRRVQHAPLHLPGDDAYTAVTKGSGKQAVATT
                     MAFVRLLKDLMRDKEFGQRIVPIIPDEARTFGMDSFFPTIKIYNPHGQNYTPVDHDLM
                     LAYRESEQGQILHLGINEAGSLAAFTAAGTAYATHGQPMIPIYVFYSMFGFQRTGDSI
                     WAAADQMTRGFLIGATAGRTTLTGEGLQHADGHSPLLASTNPAVVAYDPAYGYEIAHI
                     MKDGLRRMYGEPEDPRGRNAIYYITVYNEPYVQPAQPADLDVEGVIRGIYKLHAADTS
                     AWTHTAPRAQLLASGVGVPWALEAQKLLADDFGVAADVWSVTSWSELRRDAMACDEHA
                     FLHPEEPAKVPFVTQALTGAPGPVLAVSDYMKAVQDQIGPWVPGGLTSLGADGFGFSD
                     TRQAARRAFHIDGPSLAVRTLQVLAARGEAPIEWARTAAEKYRLLDINAGTSGSAGGD
                     A"
BASE COUNT          455 a          955 c         1041 g          472 t
ORIGIN      
        1 gtcgcgaggt gctgctcccc gttggtgacg gcgagcaggg cacctccggg gcgcagcact
       61 cggcacacct cggctagggc ggcgtcaagc gatggcacgt gatagagcat ccaggccgcg
      121 acaaccacat cgatcgagcc agtcggttgt ccggtggcct cggctggccg cacctcagcc
      181 cttacaccct gggcgcttgc ggcggccacc atcgcgggtg acgcatcggt ggcgaggtag
      241 tcgacgtctg gcagcgcgtc gacgacccgc ttggcgaaag ctcccgtgcc gcaaccgatt
      301 tccaagactg ttcgaggcga ccttgccacc aactcctcga acaggacgtc cgtcggatcc
      361 aggccagttg cgccaggccc ccacacggac atccggccgc gaagccgctc cgtggtggcg
      421 tactggtggg cgacatgtcc ctggtcaccg acgttgatcg actcgaagga ggtcaccccg
      481 gcagtatccc gccagcacag cgaataccaa tggttgggct ctccgcggct ggccacgctc
      541 aacagggcgc ccagctcaga ccattggcct tcggtgtgcg cagcaaggcc cgggaagccg
      601 cgcggcgcgg agcgggtggg agggaagccg ccgcggcaaa ggagaggcgg gcggcatacg
      661 gtatgccgcc cgccttcctc tagcgaaaac cagacctacg cgtcgccgcc cgcactgccg
      721 gaggtgccgg cgttgatgtc gagcaggcgg tacttctcgg ctgcggtgcg agcccactcg
      781 atgggggctt cgccacgcgc ggcgagcacc tgcagggtgc gcaccgccag tgacggaccg
      841 tcgatgtgga aggcccgccg cgccgcctgg cgagtgtcgg agaagccgaa cccgtcggcg
      901 ccgagcgagg tgagtccacc aggcacccac ggaccgatct ggtcctggac ggccttcatg
      961 tagtccgaga cggcgagcac ggggcccggc gcgccggtca gggcctgggt gacgaacggc
     1021 accttggccg gctcctcggg gtgcaggaag gcgtgctcat cgcacgccat cgcgtcccgg
     1081 cgcagctcgc tccacgacgt gaccgaccag acgtcagcag ccactccgaa gtcgtcggcg
     1141 agcagcttct gggcctccaa cgcccacggc acgccaacgc cagaggcgag caactgggcc
     1201 cgaggcgcag tgtgcgtcca ggccgacgtg tcggcggcat gcagcttgta gataccccgg
     1261 atgacgccct cgacatcgag gtcggcaggc tgggccggct gcacgtaggg ctcgttgtag
     1321 accgtgatgt agtagatcgc gttgcgaccg cgcgggtcct cgggctcgcc gtacatccga
     1381 cgcagcccgt ccttcatgat gtgcgcgatc tcgtagccgt aggccgggtc gtaggcaacg
     1441 accgccgggt tggtcgaggc cagcagcggc gagtgaccgt ccgcgtgctg caacccctcg
     1501 ccggtgagcg tcgtgcggcc cgccgtcgcg ccgatgagga acccgcgcgt catctggtcg
     1561 gcggcggccc agatcgagtc gccggtgcgc tggaacccga acatcgagta gaagacatag
     1621 atcgggatca tcggctggcc atgcgtggcg tatgccgtgc ccgctgcggt gaacgccgca
     1681 agggacccgg cctcgttgat gcccaggtgc aggatctgac cctgctcgga ctcgcggtag
     1741 gccaacatca ggtcgtggtc gaccggcgtg tagttctggc cgtgggggtt gtagatcttg
     1801 atcgtcggga agaacgagtc catcccgaag gtccgcgcct cgtccgggat gatcggcacg
     1861 atgcgctggc cgaactcctt gtcccgcatg aggtccttga gcagccgcac gaacgccatg
     1921 gtcgtcgcga cggcctgctt gcccgagccc ttggtgacgg cggtgtaggc gtcgtcgccg
     1981 ggcaggtgca ggggggcgtg ctggacgcga cgcgagggga ccgatccgcc gaggacgcgc
     2041 cggcgctcac gcatgtactg catcgccggg tcgctggcgt ccggccggta gtacggcggg
     2101 cggtaggggt cggcctcaag ctgctcgtcc gtgatcggga tgcgcagcag gtcgcggaag
     2161 cccttgaggt cttccagggt gagcttcttc atctggtgcg tggcgttgcg gccagcgaag
     2221 tgcgacccga gggtgaagcc cttgatggtc ttgaccagga tgacggtcgg ctggccggtg
     2281 tgctccagag cggcgcggta ggcggcatac accttgcggt agtcgtgccc gccgcggcgc
     2341 agcttgaacc acacctcgtc gtcggtccag ttctcgacca tcttgcgggt gcgcgggtcg
     2401 cgccagaaga agtgctcgcg gacgtacttg ccgtcgttgg cccggaaggt ctggtagtcg
     2461 ccgtcagagg tctggttcat gaggttggcc agtgccccgt cgcggtcggc ggcgagcagc
     2521 gggtcccacc cgcggcccca gacgaccttg atgacgttcc aaccggcgcc gcggaagaac
     2581 gcctcgagct cttggatgat cttgccgttg ccgcggaccg ggccgtcgag gcgctgcagg
     2641 ttgcagttga cgacgaaggt gaggttgtcc agctcctcgt tggcagccag ctgcagcagc
     2701 ccacgcgact cgggctcatc catctcgccg tcgccgagga acgcccacac gtgctggtcg
     2761 ctcgtgtcct tgatcccgcg gttgagcagg tagcggttga actgcgcctg gtagatcgcg
     2821 ttcatcgggc cgatgcccat cgacaccgtc gggaactgcc agaagttcgg catgagccgc
     2881 gggtgcgggt aggacgacag cgcccggatc ttgccgtcga cga
//
LOCUS       DAORPW010000265         2919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_11917_length_2919_cov_724.388966, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000265 DAORPW010000000
VERSION     DAORPW010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11917_length_2919_cov_724.388966"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLB86_13030"
     CDS             <1..380
                     /locus_tag="PLB86_13030"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010080160.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRC66114.1"
                     /translation="RLTPLARVHTMAVVGSDPVLMLTGPIPATATVLHRTGLSLAEID
                     TVEINEAFASVVLAWADETDADLDRVNPQGGALALGHPLGATGARLMTTLVHRMGRDH
                     LRYGLQLMCEGGGMANATVLERA"
     gene            382..1917
                     /locus_tag="PLB86_13035"
     CDS             382..1917
                     /locus_tag="PLB86_13035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC66115.1"
                     /translation="MTSPAEEAHGIPSAGSPPAPPAEERGIPSAELPSAPPPRGLVPC
                     LVYAALSGSVVAGLGNPIVVPVNRTLHVSLEAAQWTLTMSLLMGVVATPIVARLADGR
                     LRRRVLVGALATVAIGSLVGALVPNFPGLMVARALHGLGYALVALTVGIAREHLHGPQ
                     LHRTLAVLSTSLAVGAGVANPTVGLMVAIWDYRAGFVFAFLVAAAGAWWTFRTVPEPK
                     AGIAPVHTDILGAVLLCSGLGFGLLAITHGVSWGWTSSGVLGLAGGAVVLLLAWVLIE
                     VRLAHPLVDVRLAVAPGILGANLAALLIGCSVFAGAAAGIILTQQTPSGGVGLGHNVA
                     ITGLLMGPMALGSLAGPPIARRLVRRLGERSVLPIGTLSCALGFGIFARWHHTELEVL
                     LMMGLMGLGVGITYSILPSLVVARVPMQRTASATGVNQVLRLLGGAVGSAGLAAVLAA
                     HVDGASGLPAESGYVMASLLAAGVSLVAAPLSYWLVPDAPAGASEAGGERTAYLPEEP
                     VTP"
     gene            1922..2062
                     /locus_tag="PLB86_13040"
     CDS             1922..2062
                     /locus_tag="PLB86_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66116.1"
                     /translation="MLAAGVRCTAPEDVAEQLIGLQEELGMTHFVDKPVFGTQVIPAV
                     TR"
     gene            2059..2895
                     /locus_tag="PLB86_13045"
     CDS             2059..2895
                     /locus_tag="PLB86_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC66117.1"
                     /translation="MKLETGQVAVVTGAASGIGRGLVEAFVARGLSVVGVDVEAGALD
                     AMVAEVNGGAGDGTGESGTGQVVGRVVDVRDPEALLALAADVVATFGRVDVVCNNAGV
                     ITSRLSTWEQPHSDVQWTTEVNFYGVVNGVRAFVPHFIAAGQGHVVNTASVAGLSTIP
                     GGGQAAYSASKHAVVGLSETLALELATVAPEVGVTVLCPGQVPTKIRDARRNRPADRP
                     ETGGSTVLPDFRLTMDVATPAEVAEMVLAAIEDGLFYLVTAPDAGQWARTRAQGVIDG
                     LS"
BASE COUNT          366 a          996 c         1077 g          480 t
ORIGIN      
        1 atcggctgac cccgctcgcg cgcgtacaca ccatggccgt cgtcggctcc gacccagtcc
       61 tcatgcttac tggcccgatc cccgccacgg cgacagtcct gcatcgaacc gggctgtcgc
      121 tggccgagat cgacacggtc gagatcaacg aagccttcgc ctccgtcgtc ctcgcctggg
      181 ccgacgagac cgatgctgac ctggaccgcg tcaatcccca gggcggggcc ctcgctctcg
      241 gccacccgtt gggtgccacc ggcgcccggc tgatgacgac cctcgtccac cgaatgggcc
      301 gcgatcacct gcgctacggc ctgcagctca tgtgcgaggg tggcggaatg gccaacgcca
      361 ccgtcctgga acgcgcctga ggtgacctca ccggccgagg aggcgcacgg cataccctcg
      421 gcagggtcgc cacccgcacc gccggcggaa gagcgcggca taccctcggc tgagctgccg
      481 tccgctcctc cacctcgcgg gctggtcccc tgcctggtgt atgccgctct cagcggctcg
      541 gtcgtcgcgg gcctgggcaa cccgatcgtc gtcccggtca accggaccct gcacgtctcg
      601 ctcgaggcgg cccaatggac gctgacgatg agcctgctca tgggggtcgt tgccaccccg
      661 atcgtcgcca ggctggccga cgggcggctg cggcggcgag tgctcgtcgg cgcgctggcc
      721 acggtggcca tcgggtcgct cgtgggggcg ctcgtgccga acttccccgg gctgatggtg
      781 gcgcgggcgc tgcatgggct cggctatgcg ctcgtcgcgc tcaccgtcgg gatcgcccgc
      841 gagcacctgc atgggccgca actgcatcgg accctggccg tgctctcgac gagcctggcc
      901 gtcggtgccg gcgtggccaa cccgacggtg ggcctcatgg tggcgatctg ggactaccgg
      961 gccgggttcg tcttcgcgtt cctcgtcgcg gcagcggggg catggtggac gttccgcact
     1021 gtccccgagc cgaaggcggg catcgcgccg gtgcacaccg acatcctcgg ggccgtgctg
     1081 ctgtgcagcg ggctgggttt cgggctgctg gcgatcaccc atggggtgtc ctggggctgg
     1141 acgtccagcg gagtgctcgg gttggccggt ggggccgtcg tgctgttgct ggcctgggtg
     1201 ctcatcgagg tgcggttggc tcacccgttg gtcgatgtcc ggctcgccgt cgcaccgggg
     1261 atcctcgggg cgaacctcgc cgcgctgctc atcggctgct cagtgttcgc cggggctgca
     1321 gcggggatca tcctcaccca gcaaacgccc tccggcggag tcggtttggg ccacaacgtc
     1381 gcgatcaccg gcctgctcat ggggccgatg gcgctgggca gcctggccgg tccgccgatc
     1441 gcccgacggc tggtccggcg cctcggcgag cggtcggtgc tgccgatcgg aacgctgtcc
     1501 tgcgcactgg gtttcgggat cttcgcccgc tggcatcaca ccgagttgga ggtcctgctg
     1561 atgatgggcc tgatgggcct cggcgtcggc atcacctatt cgatcctgcc cagcctggtt
     1621 gtcgcccggg tgccgatgca gcggaccgcc agcgcgaccg gggtcaacca agtgctccgg
     1681 ctgctcggcg gagcagtggg gtcggcgggg ttggccgccg tgctggcggc gcacgtcgac
     1741 ggtgcgagcg gattgccggc ggagagcggc tatgtcatgg ccagcctgct cgccgcgggg
     1801 gtgtctctgg tggccgcacc gctgtcctac tggctggtcc ccgacgcccc ggcaggtgcg
     1861 tcggaggcgg gcggggagcg gacggcatac ctcccggagg agccggtcac gccctgaacg
     1921 ggtgttggct gcgggagtcc ggtgcaccgc acccgaggac gtcgctgagc agttgatcgg
     1981 actacaggag gagttgggaa tgacgcactt cgtcgacaaa ccggtgttcg gcacccaggt
     2041 catcccggcg gtgacccggt gaagctcgag acgggccagg tcgcggtcgt gaccggcgcc
     2101 gcgagcggga tcgggcgcgg tttggtcgag gccttcgtgg cgcggggact gtcggtcgtc
     2161 ggggtcgacg tggaagccgg cgcacttgac gcgatggtgg ccgaggtcaa cggcggcgcg
     2221 ggtgacggga cgggcgaatc agggactggg caggtggtcg ggcgggtcgt cgacgtccgc
     2281 gaccccgagg cgctgttggc gctggctgcc gatgtcgtgg cgaccttcgg ccgggtcgac
     2341 gttgtgtgca ataacgccgg cgtcatcacg tcgcgtctgt cgacatggga gcagccgcac
     2401 agcgacgtgc agtggacgac cgaggtcaac ttctacggcg tcgtcaacgg ggtgcgagcc
     2461 ttcgtgccgc acttcatcgc cgccggtcag gggcatgtcg tcaacaccgc ctccgtcgcg
     2521 ggactgtcga cgatccccgg aggcgggcag gcggcataca gcgcctcgaa gcacgccgtc
     2581 gtcgggctgt cggagacgct cgctctggag ttggccaccg tcgcgcccga ggtgggcgtc
     2641 accgtgctgt gccccggcca ggtgcccacc aagattcgcg acgcgcggcg caaccggccc
     2701 gccgaccggc cggagacggg gggcagcaca gtgctgccgg acttccggct caccatggac
     2761 gtggcgactc ccgccgaggt agccgagatg gtgctggcgg ccatcgagga cgggctcttc
     2821 tacctcgtca ctgctcctga cgccggccag tgggcccgca cccgcgccca aggagtcatc
     2881 gacggcctgt cctgagttga ccgtcggctt gcgatgccc
//
LOCUS       DAORPW010000266         2908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_11982_length_2908_cov_846.092184, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000266 DAORPW010000000
VERSION     DAORPW010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11982_length_2908_cov_846.092184"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..871)
                     /locus_tag="PLB86_13050"
     CDS             complement(92..871)
                     /locus_tag="PLB86_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRC66118.1"
                     /translation="MPDSPTVFALLVAAGLLAGWVDAVVGGGGLIQLPALLLGLPGAA
                     PAQLLATNKIASICGTSVSAATYYRRVRPDLRTALPMAGAAYVGAIGGALIGLHIPKS
                     AFNPVILALLVVVGAYTLLRPDLGTVTELRHSGTRHTSYAVLVGLVIGAYDGALGPGT
                     GSFLVFALVGWLGYAFLQASAKAKIANFATNLGALTVFVPQGQVLWVTGLAMGVANLV
                     GGYLGARTAVRLGSGFVRAVFMMVVGAFIVRLGGSVFGVWP"
     gene            complement(864..1655)
                     /locus_tag="PLB86_13055"
     CDS             complement(864..1655)
                     /locus_tag="PLB86_13055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRC66119.1"
                     /translation="MTSGQMPGAVEAVIFDWGGTITPWHTVDLREQWHVFARGMGAIA
                     CSLNDTASAVLKAEDAAWRRGREEGTSARLDDILRTAGLDPDHPATHAGLAAYREFWE
                     PHTFTHPNIPEVWEWLRDNGIRVGVLSNTIWSRDYHREIFERDGVLHLVDADVYSSET
                     PWVKPRPEIFRAAAAAVGADPSACVYVGDRSYEDVHGPQSVGMRAIWIPHSDIPAEQQ
                     VSHEATPDAIAHELTDLVDIVSAWSFDGSSAGSSFGGPESPLGDA"
     gene            complement(1652..>2908)
                     /locus_tag="PLB86_13060"
     CDS             complement(1652..>2908)
                     /locus_tag="PLB86_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRC66120.1"
                     /translation="DAHRDAYIKIFDRLGFHYVIVQAMAGAMGGSKSEEFLATAENGE
                     DTYVRCESCGYAANVEAVRVPAPAAIATEGMPSALVIDTPGTPTIESLVAFLNEAHPR
                     ADRAWSAADTLKNVVVLLSYPDGRTEPLVIGVPGDREVDEKRLAAQIEPAVAVPFTDA
                     EFAAHPGLVRGYIGPEALGADPERASATGIRYLTDPRVSEGSAWVTGANLDGKHVIGL
                     VAGRDFRSDGTIEAAEVKVGDVCPSCGQGDGLVAARGIEMGHIFQLGTKYAKALDLKV
                     LDESGKLVTVVMGSYGIGVSRAVACVAEGNHDQFGLVWPREIAPADVHVVATGKDEEV
                     FVAADTLARELAAQGLEVVYDDRRGVSPGVKFKDSELVGVPTIVVVGRGLVDGKVEIK
                     DRRSGERREVAVGEAVAEVCREVRGS"
BASE COUNT          479 a         1052 c          970 g          407 t
ORIGIN      
        1 ccccgttttc ccacggagat agcgctcgca cggtcatctt tgcggcccgc tcccccgatt
       61 ctccccggag atagcgctcg cacggtcatt tctacggcca caccccgaag accgagccac
      121 cgaggcgcac gatgaaggcg ccgaccacca tcatgaacac cgcccgtacg aagccactcc
      181 ccagccgcac ggccgtgcgc gcaccgaggt aaccgccgac aaggttcgcc acacccatcg
      241 ccagtcccgt cacccacagc acctggccct gcggcacgaa caccgtcagc gccccgaggt
      301 tggtggcgaa gttcgcgatc ttggccttcg cgctggcctg caggaacgcg taccccagcc
      361 accccaccaa cgcgaacacc aggaacgacc ccgtccccgg ccccagcgcc ccgtcgtagg
      421 cgccgatgac caggccgacg aggacggcat acgaggtatg ccgcgtgccg gagtgccgca
      481 gttcggtcac cgtcccgagg tcgggacgca gcagggtgta ggccccgacg acgacgagca
      541 gcgcgaggat gaccgggttg aacgcgctct tgggaatgtg cagcccgatg agcgccccac
      601 cgatggcacc gacgtaggcg gcgccggcca tgggcagggc ggtgcgcagg tccggccgca
      661 cgcgccggta atacgtcgcc gcgctcaccg aggtcccgca gatcgaggcg atcttgttgg
      721 tggccagcag ttgggccggc gcggcgcccg ggagcccgag gagcaaggcg ggcagttgga
      781 tcaacccgcc gccacccacc accgcgtcga cccagccggc caacaggcca gccgcgacga
      841 gcagcgcgaa gacggttggg ctgtcaggca tccccgagcg gggactccgg cccgccgaac
      901 gagctgcccg cggacgaccc atcgaaggac caggccgaca cgatgtcgac caggtcagtc
      961 agctcgtgcg cgatcgcatc gggggtggct tcgtgcgaga cctgctgctc ggccgggatg
     1021 tccgagtgcg ggatccagat cgcacgcatc ccgacggact ggggcccgtg gacgtcctca
     1081 taggagcggt caccgacata gacacaggcc gaaggatcgg ccccgaccgc tgccgccgcg
     1141 gcacggaaga tctccggtcg cggtttcacc cacggcgtct cgctggagta gacgtcggcg
     1201 tcaacgaggt gaagcacccc gtcgcgctcg aagatttcgc ggtggtagtc gcgcgaccag
     1261 atcgtgttgg acaggacccc aacgcggatt ccgttgtcgc gcaaccactc ccagacttcc
     1321 ggaatgttcg ggtgagtgaa cgtgtgcggc tcccagaact cccggtatgc cgccagcccg
     1381 gcatgcgtcg ccgggtggtc cggatcgaga ccggccgtgc gcaagatgtc atcgagccgc
     1441 gcgctggtcc cctcctctcg accacgccgc cacgccgcgt cctctgcctt gagcaccgcc
     1501 gacgcggtgt cgttcagcga gcaggcgatc gcacccattc cccgagcaaa gacgtgccac
     1561 tgctcacgca ggtcgacggt gtgccacggg gtgatcgtgc cgccccagtc gaagatgacg
     1621 gcctcgaccg cgccgggcat ctggccggac gtcacgagcc gcggacctcg cggcatacct
     1681 cggcgacggc ctcgccgacc gcgacctccc gtcgctcccc cgaccggcgg tccttgatct
     1741 cgaccttccc gtcgaccagg ccgcgcccga cgacgacgat cgtcggaacg ccgacgagct
     1801 cggagtcctt gaacttcacg ccgggcgaca caccacggcg gtcgtcgtag acgacctcca
     1861 ggccctgggc ggccaactcg cgagccaacg tgtcagcggc gacaaagacc tcctcgtcct
     1921 tgccggtggc gacgacgtgg acatcggcgg gcgcgatctc ccgcggccag accagaccga
     1981 actggtcgtg gttgccctcg gcgacgcagg cgacggcgcg ggagaccccg atgccgtagg
     2041 aacccatgac gacggtgacg agcttgcccg actcgtcgag aaccttgagg tccagcgcct
     2101 tggcgtactt cgtcccgagt tggaagatgt gccccatctc gatgccacgc gccgcgacta
     2161 gtccatcgcc ctggccacac gatgggcaga cgtcgccgac cttgacctcg gcggcctcga
     2221 tggtgccgtc ggagcggaag tcgcgcccgg cgaccagccc gatgacgtgc ttgccgtcga
     2281 ggttcgcgcc ggtcacccac gcactgccct cggagacccg ggggtcggtg agataacgga
     2341 tcccggtagc ggatgcccgc tcagggtcgg cgccgagcgc ctccggcccg atgtagccac
     2401 gcaccagacc cgggtgcgcc gcaaactcgg cgtcggtgaa gggcacagcg acggccggct
     2461 cgatctgagc ggccagtcgc ttctcgtcga cctcgcggtc accagggacg ccgatgacca
     2521 gcggctccgt gcgcccgtcg gggtagctca gcagcacgac gacgttcttg agcgtgtcgg
     2581 cagcgctcca cgcgcggtcg gcccgggggt gcgcctcgtt gaggaaggcc accaggctct
     2641 cgatcgtcgg ggtgccaggc gtgtcgatga cgagggcgga cggcatacct tccgtcgcga
     2701 tcgccgcggg agcggggacg cgcacggcct cgacgttcgc ggcatacccg cagctctcgc
     2761 agcggacgta ggtgtcctcg ccgttctccg cggtggcgag gaactcctcg ctcttgctgc
     2821 cgcccatggc gccggccatc gcctggacga tgacgtagtg gaagccgagc cggtcgaaga
     2881 tcttgatgta ggcgtcgcgg tgggcgtc
//
LOCUS       DAORPW010000267         2861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_12290_length_2861_cov_612.990021, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000267 DAORPW010000000
VERSION     DAORPW010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12290_length_2861_cov_612.990021"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLB86_13065"
     CDS             complement(<1..692)
                     /locus_tag="PLB86_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRC66121.1"
                     /translation="MTDIEQLNPGLAGLGRYEYGWSDADTAGSTARRGLNEDVVRNIS
                     GLKNEPQWMLDLRLKSLRLFDRKPMPTWGSDLSGIDFQNIKYFVRSTEKQATSWEDLP
                     ADIKNTYDRLGIPEAEKQRLIAGVAAQYESEVVYHQIREDLEEKGVIFVDTDTGLREH
                     GDIFKEYFTTVIPAGDNKFASLNTAVWSGGSFIYVPPGVKVDIPLQAYFRINTENMGQ
                     FERTLIIADEGS"
     gene            complement(689..1399)
                     /locus_tag="PLB86_13070"
     CDS             complement(689..1399)
                     /locus_tag="PLB86_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRC66122.1"
                     /translation="MIIEPAERHTEQETRTRARVLAAVSERGPVSASALGRLLGLTPA
                     GVRRHLEALAEHGAVSEHDPVTAATGRGRGRPAREWVVAEAGHASLTSGYDDLAAQAL
                     RFLRDAAGPDAVAAFARARSADVEARYAADLASVGPSPQARVEALVSVLTRDGFAASA
                     RPVGEGARTGVQVCQGHCPIHHVAAEFPQLCQAETDAFSRLLGVHVQRLSTLARGQHV
                     CTTFVPIPAVPESKGVTS"
     gene            1514..1879
                     /locus_tag="PLB86_13075"
     CDS             1514..1879
                     /locus_tag="PLB86_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66123.1"
                     /translation="MSHPVVAVYARARGGRWELAGHADLLMPRILLADQELATALAAH
                     HTPVETVAHLSWMDRHEVRGELIDIVDVGPIGSWQDHDGGTGLELDRPSTGNPIFIPP
                     GPGGDGFWCMLFPWASFCG"
     gene            1897..>2861
                     /locus_tag="PLB86_13080"
     CDS             1897..>2861
                     /locus_tag="PLB86_13080"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC66124.1"
                     /translation="METLRARAGAVTVAVALDLLVWNGAVGTVAGGSIAWWLPVAAVA
                     LIHQSLWWLASRPYAVLTAQCAFALVSTVVPQWQPFSGLLIATYAVSSRPRGRHTLAV
                     LVGLGVAMLSHSYGSARLTLDPLRSTVTLLALWGALAIAVWAVGARHRRTVDRAVAAG
                     ETLSAQARAGVANERLRIARELHDGVCGAVTAIQLQAAGASALSPTHPEAAGPSLVAI
                     QSSAERTLLELRRVLGDLRRDAVDDGGASSIEAPSLDEIPQLVELARAAGLHVTLTQT
                     VDPLTRLDPLAEATVVRVVQEGLTNAVKHGPRGRCQVSVSVGPDGA"
BASE COUNT          414 a          958 c         1018 g          471 t
ORIGIN      
        1 taggagccct cgtcggcgat gatcagcgtc cgctcgaact ggcccatgtt ctcggtgttg
       61 atccggaagt aggcctgcag cgggatgtcg accttgacgc ccggcgggac gtagatgaac
      121 gaaccgcccg accagacagc ggtgttgagc gaggcgaact tgttgtcgcc cgccgggatc
      181 accgtcgtga agtactcctt gaagatgtcc ccgtgctcgc gcaggccggt gtcggtgtcg
      241 acgaagatga cacccttctc ctccaggtcc tcgcggatct ggtggtagac cacttcgctt
      301 tcgtactgcg cggcaacgcc ggcgatgagg cgctgcttct cggcctcggg gatgccgagc
      361 cggtcgtagg tgttcttgat gtccgccggc aggtcctccc acgaggtggc ctgcttctcc
      421 gtcgagcgga cgaagtactt gatgttctgg aagtcgatcc cgctgaggtc actcccccac
      481 gtcggcatcg gcttgcggtc gaacagccgg agcgacttca ggcgcaggtc gagcatccac
      541 tgcggctcgt tcttgaggcc cgagatgttg cggacgacgt cctcgttgag gccacgccga
      601 gcggtggacc cggcagtgtc ggcatccgac cagccgtact catagcggcc gagcccagcc
      661 aggccggggt tgagctgctc gatatcggtc acgacgtgac acctttcgat tcggggacgg
      721 cggggatcgg gacgaaggtg gtgcagacgt gctgaccgcg agccagggtc gagagtcgct
      781 gcacgtgcac gccgagcaac cgtgagaacg catcggtctc ggcctgacac aactggggga
      841 actcggcggc gacgtgatgg atggggcaat ggccctgaca gacctggacc ccggtccgcg
      901 cgccctcacc gaccggacga gccgaggcgg cgaacccgtc cctggtgagc actgagacga
      961 gcgcctcgac ccgcgcctgg ggcgagggac ccaccgaggc gaggtcggcg gcatacctgg
     1021 cctcgacgtc agccgatcgc gcgcgggcga aagcggcgac ggcgtcggga ccggcggcgt
     1081 cccgcaggaa gcgcagggct tgggcggcca ggtcgtcgta accggaagtc agggacgcgt
     1141 gcccggcctc ggcgacgacc cattcgcgcg ccggacgacc ccgcccgcga ccggtggcgg
     1201 ccgtgaccgg gtcgtgttcg gagacggcgc cgtgttcggc gagggcttcg aggtgccggc
     1261 ggaccccggc gggcgtcagc ccgagcaggc ggcccagcgc ggaggcggac accgggcccc
     1321 gctcggacac cgcagcgagg actcgcgcac gggtgcgcgt ctcctgctcg gtatgccgtt
     1381 cggccggctc gataatcaca acgtaagtat gtcgtaattc gggggaagtg ccaaacagcg
     1441 ccgccaggta tcccgccgac ccgtcacgtc tcttgctttt gcgacacacc agtcccatcc
     1501 gaacggagag accatgtcgc atcctgtcgt cgccgtctac gcccgagccc gcggtggtcg
     1561 gtgggagttg gccgggcatg ccgacctcct gatgccccgg atcctgcttg ccgaccagga
     1621 gctcgcgacg gctctcgctg cccaccacac gccggtcgag acggtggccc atctgagttg
     1681 gatggaccgt cacgaggttc gcggcgaact catcgacatc gtcgacgtgg gcccgatcgg
     1741 ctcgtggcag gaccacgacg gtgggaccgg gttggagctt gatcgaccga gcaccgggaa
     1801 cccgatcttc atcccgccgg gcccgggcgg cgacggcttc tggtgcatgt tgttcccctg
     1861 ggcgagcttc tgtggttgac ggaaggcgtt gctgacatgg agaccttgcg cgcacgcgcc
     1921 ggtgccgtca ccgtggccgt cgctctggac ctgctcgtct ggaacggcgc ggtcggcact
     1981 gtggcgggcg gatcgatcgc ctggtggctc cccgtcgcgg ccgtcgcgtt gatccaccaa
     2041 agcttgtggt ggctcgcgtc gcggccgtat gccgtgctca cggctcagtg cgcgttcgcc
     2101 ctggtcagca ctgtcgtccc ccagtggcag ccgttctcgg ggttgctcat cgcgacgtat
     2161 gccgtctcgt cccggccgcg ggggcggcat accctcgcgg tgctcgtcgg cctgggagtg
     2221 gccatgctct cccatagcta cggctcggcg cggctcaccc tcgaccccct tcgctcgacc
     2281 gtcactctgc tcgcgctctg gggcgcgctg gccatcgccg tgtgggccgt aggagcgcgg
     2341 caccgacgga cagtcgatcg agcggtggcg gcgggtgaga cgttgtcagc ccaggcgcga
     2401 gcaggcgtgg ctaatgagcg gctgagaatc gcgcgcgagt tgcacgacgg ggtgtgcgga
     2461 gcggtcaccg cgatccagct gcaagcggcc ggcgcctcgg ccctcagccc gacccacccg
     2521 gaggccgctg gccccagcct ggtggccatt cagagcagtg ccgagcgcac cctgctggag
     2581 ctgcgccggg tcctgggtga tctgcgtcgc gacgctgttg atgatggcgg cgcgtcgagc
     2641 atcgaggcac ccagcctgga cgagattccc cagttggtcg agctggcccg cgcggctggg
     2701 ctgcacgtca cgctgaccca gactgtcgac ccactgaccc ggctggaccc gctcgccgag
     2761 gcgaccgtcg tccgggtggt ccaggaggga ctcaccaacg cggtcaaaca tggccctcgc
     2821 gggcgctgcc aggtctcggt gagcgtcggc ccggacggtg c
//
LOCUS       DAORPW010000268         2859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_12298_length_2859_cov_671.974322, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000268 DAORPW010000000
VERSION     DAORPW010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12298_length_2859_cov_671.974322"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLB86_13085"
     CDS             <1..204
                     /locus_tag="PLB86_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66125.1"
                     /translation="LRNVPDEVGDALEELASRASMSVSAYAVRELSAAVAFRSNADLL
                     WTQPQVEVDIDDVVAAVRSGRDR"
     gene            132..602
                     /locus_tag="PLB86_13090"
     CDS             132..602
                     /locus_tag="PLB86_13090"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRC66126.1"
                     /translation="MDPAAGRGRHRRRRRGGPFGARSLIVVDTSVVVAALLGHDPARH
                     ALDDRRLVAPHLIDAEVAHALRGLVLGGRVTADDGERLLSVWQHLAVDRLPMTGLLPR
                     VWALRDALTAYDAMFVAAAEALRVPLVTCDRQLAHAGAARCTVEIVPGASSMPS"
     gene            599..2023
                     /locus_tag="PLB86_13095"
     CDS             599..2023
                     /locus_tag="PLB86_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRC66127.1"
                     /translation="MTTAVVVGSGPNGLAAAIRLAQDGLEVTVVEAYERPGGGTRTSE
                     LTLPGLLHDDCAAFHPTGVASPFFRSLGLERHGLRWLWPEVDLAHPLDDGRAGLAARD
                     LSITNESLGVDAARWRRVFDAPIRGFDDLISEVFQPVVHLPSHPVTLARFGTRALMPA
                     TWTAGQFKDDPARALFMGVAAHSFSQLTTPLSSSVGLLLTAAAHAVGWPVAEGGTESI
                     TRALLAELAELGGTVVTDTRVRSLDGLADLVGSAPDVVMLDTAPAGVLEIVGDRLPSR
                     VRAALSRYTYGPAAFKVDFSIEGDIPWTNPDCRRAGTLHLGGAAEQIVASEAATTSGR
                     MPDRPFVLLGQQYLIDPTRSVGTTNPVYAYAHVPHGYEGDATDAVIAQIERFAPGFHD
                     RILAVSTRNPAGLEAYNPNYVGGDIAAGANTARQIAFRPRVTSNPYSLGVKGVYLCSS
                     ATPPGGGVHGMCGLYAANTALSRL"
     gene            2082..2858
                     /locus_tag="PLB86_13100"
     CDS             2082..2858
                     /locus_tag="PLB86_13100"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC66128.1"
                     /translation="MTTTPEGIPCWYELGTSDLNAAAAFYADVLGWTIADAGMPGMDY
                     RLGTATDGGMVSGMMALEGGEAAPPPYWMIYFTTEDCDARVAAIIEAGGTMLAAPADI
                     PGTGRFAVVADPQGAAFGLLRPLPMDAPMEHPAFDQQAPGHGNWHELMSSDPEAGLAF
                     YAEQFGWTKGDTMDMGEMGTYQIFGGGGQDLGGMMGLGNSPVSAWLPYFGSTGIDQSV
                     ERIVAGGGTIHHGPAEVPGGAWIALATDPQGAWFAVVGPR"
BASE COUNT          396 a         1047 c          966 g          450 t
ORIGIN      
        1 ctgcgcaacg tgcccgatga ggtgggagac gcgctggaag agctggcttc ccgtgcctcc
       61 atgtccgtca gcgcgtatgc cgtgcgcgag ctctccgccg cagtcgcctt ccggtccaac
      121 gccgacctgc tgtggaccca gccgcaggtc gaggtcgaca tcgacgacgt cgtcgcggcg
      181 gtccgttcgg ggcgcgatcg ttgatcgtcg tcgacacgtc ggtggtggtt gcggcgctcc
      241 tcggccatga ccccgcccgg cacgccctcg atgaccgtcg attggtggca ccacatctca
      301 tcgatgccga agtggcacat gccctacggg gtctcgtcct gggcgggagg gtcacggcgg
      361 acgatggcga gcggctgctg tcggtgtggc agcacctcgc cgtagaccga ctgcccatga
      421 ctgggctgct cccccgagtg tgggcactgc gtgacgcgct cacggcatac gacgcgatgt
      481 ttgtcgccgc ggccgaggcc cttcgcgtcc ctcttgtcac ctgtgaccgg cagctggctc
      541 acgcgggtgc cgcacggtgc accgtcgaga tcgtgcccgg ggcgagctcg atgccgtcat
      601 gaccacggcc gtggtggtcg gcagcggacc caacggactc gcggcggcaa tccggctggc
      661 ccaggacggc ctggaggtca ccgtcgtcga ggcctacgag cggcccgggg gcggcacccg
      721 gaccagcgag ctaaccctgc ccggtctgct gcacgacgac tgtgccgcgt tccacccgac
      781 cggcgtcgcc tcgccgttct tccggtctct gggtctggag cgacacgggc tgcgctggct
      841 ctggcccgag gtcgacctcg cccaccctct ggacgacggc cgggccgggc tggccgcccg
      901 cgacctgagc atcaccaacg agtccctggg tgtggatgcg gcccgctggc ggcgcgtgtt
      961 cgatgccccg atccgggggt tcgacgacct catcagtgag gtctttcaac cggtcgtcca
     1021 cctgccgtcc catccggtca ccctggctcg gttcggcacc cgggccctca tgccggcgac
     1081 ctggacggcg gggcagttca aggacgatcc cgcccgagcc ctcttcatgg gggtggccgc
     1141 gcactccttc agccagctga ccaccccgct gagcagttcg gtcggcctgc tgctgaccgc
     1201 ggccgcccat gccgtcggct ggccggtggc cgagggcggc acggagtcga tcactcgggc
     1261 cctgctcgcc gagctagccg agctcggcgg cacggtggtg accgacaccc gcgtccggtc
     1321 gctggacggc ctggccgacc tggtcggctc cgcgcccgat gtcgtcatgc tggacactgc
     1381 tcccgccggc gtcctggaga tcgtcggcga ccggctgccg tcccgggttc gcgccgcgtt
     1441 gtcgcgctac acctacgggc cggccgcgtt caaggtcgac ttctcgatcg agggcgacat
     1501 cccttggaca aacccggact gccgccgagc cgggacgctg cacctcgggg gcgccgccga
     1561 gcagatcgtc gcatccgagg ccgccacgac gagcggtcgg atgcctgatc ggccgttcgt
     1621 cctgctcggc cagcagtacc tcatcgaccc gacccgatcc gtgggaacca ccaacccggt
     1681 gtatgcctac gcccacgtgc cgcacggcta tgagggcgac gcgaccgacg cggtgatcgc
     1741 ccagattgag cggttcgccc ccggtttcca cgatcggatc ctggcggtct ccacgcggaa
     1801 ccccgccggg ctcgaggcct acaaccccaa ctatgtggga ggagacatcg ctgccggcgc
     1861 caacaccgcc cgccagatcg ccttccgacc gcgggtcaca tcgaacccgt actccctcgg
     1921 cgtcaagggt gtctacctgt gctcctcggc cacgcctccc ggcggcgggg tgcacggcat
     1981 gtgcggcctg tatgccgcca acacggctct cagccgcctc tgaccagccc gtccctcccc
     2041 ccaccgttgc cttcgcaagg cggcggtggc aggctgcgcg gatgacgacc acacctgagg
     2101 ggatcccctg ctggtacgag ttggggacga gcgacctgaa cgcagccgcc gccttctacg
     2161 ccgacgtgct cggctggacg atcgccgacg ccggaatgcc ggggatggac taccggctcg
     2221 gcaccgcaac ggatggcggc atggtcagcg gcatgatggc cctggagggc ggcgaggccg
     2281 caccgccgcc ctactggatg atctacttca ccaccgaaga ttgcgacgcg cgggtcgcag
     2341 ccatcatcga ggctggcggc acgatgctcg cggcccccgc cgacatcccc ggcaccggcc
     2401 ggttcgcggt cgtcgctgac ccgcaagggg ccgctttcgg gctgttgcgg ccgctgccga
     2461 tggacgcgcc catggagcac cccgccttcg atcaacaagc ccccgggcac gggaattggc
     2521 acgaattgat gagctcggac ccggaggccg gtctcgcctt ctacgccgaa cagttcggct
     2581 ggaccaaagg cgacacgatg gacatgggcg agatgggcac ctatcagatc ttcggagggg
     2641 gcgggcagga cctcggcggc atgatggggc tcggcaacag ccccgtctcg gcctggcttc
     2701 cctacttcgg ctccaccggg atcgaccagt ccgtcgaacg gatcgtcgcc ggcggcggca
     2761 ctattcacca cggcccagcc gaggtgcccg gcggcgcctg gatcgccctg gccaccgacc
     2821 ctcagggcgc ctggttcgcc gtcgtcggac cgcgctgat
//
LOCUS       DAORPW010000269         2787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_12818_length_2787_cov_879.734627, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000269 DAORPW010000000
VERSION     DAORPW010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12818_length_2787_cov_879.734627"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..747
                     /locus_tag="PLB86_13105"
     CDS             121..747
                     /locus_tag="PLB86_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HRC66129.1"
                     /translation="MATELVEKLLEVGIDGRGPVKSAHEVAVAARAEVRSDDAAIKVI
                     IRKHVRAAAVGGFVTGLGGFVTMPVALPANVAEFYLIATRMVAAIADVRGYDVTDPQI
                     RTAVLLTLVGVDANEVVKKAGAIPGVSFGPGGTLTALASQRLPGPALMVLNKAIGFRL
                     VAQAGRTLLGRLGKAVPVVGGAIGAGVDGYLLSRIADGATREFPRRAD"
     gene            852..1313
                     /locus_tag="PLB86_13110"
     CDS             852..1313
                     /locus_tag="PLB86_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRC66130.1"
                     /translation="MAAARGASSARWGMIRALGLALRAATRPGAPGMGERLSALPRMV
                     RAAMRGEYAGVTRGRLLGMLGALLYVVSPVDLVPESLFTIFGLADDALLVTWLASSLV
                     NDTEAFLTWERGLSRAGATSAAGGPQDAPAGERTTPEDSSAHWTVQSHVVR"
     gene            1487..2689
                     /locus_tag="PLB86_13115"
     CDS             1487..2689
                     /locus_tag="PLB86_13115"
                     /inference="COORDINATES: protein motif:HMM:NF025200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HRC66131.1"
                     /translation="MTTTIRITDPVDLVKVVPYQLGYHPERSLVLIGLRRKQIGLVQR
                     LDLPTLPRDCEAAADLMAGHLRKDGCTGAVLVLYEDTEGEGAVAAAALVARLQVGRFD
                     LVEHVVVRDGHVYFGALAGRADRPVSGVLLPPDDQVPAVADYVAHGRRPAPSRGSLDE
                     RLAPEDDALSERVRLAAARLGVLRPVLGSRPKAIADWGAFLDVGDDAWFAGTLPSAAA
                     TARMCHSLRDLHLRDLVTAWLCPGTLELTAFDEDLRALAVEHLPAGRAWPEAYAAAGH
                     SPAAGLVADEAPAARPRGGAGSRRRRAASSKPRRVVTAVAGPRTDEDDRLVERLCWLA
                     RHTPEVHASAVLSVLASVTWHLGDGTLTRVALDRALALDPGYRLALLIERMVDLAIRP
                     GRALSSTG"
     regulatory      2735..>2787
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
BASE COUNT          355 a          979 c         1013 g          440 t
ORIGIN      
        1 gggcggaagg acagcgtcgt ggggacattc ggcaagggcg agtcgagcga gttgtcgagc
       61 gtgcctcggg gcgctctcga acgggccgcc gacagcgagt cggagggggg cctcaccaag
      121 gtggccaccg agttggtcga gaaactgctc gaggtcggga tcgacggccg cgggccggtc
      181 aagtcggcgc acgaggtcgc cgtggccgcg cgggcagagg tccgcagcga tgacgccgcg
      241 atcaaggtga tcatccgcaa gcacgtccgg gccgccgccg tcggagggtt cgtcacgggg
      301 ttgggcggat tcgtgacgat gcccgtcgcc ctgccggcca acgtcgcgga gttctacctc
      361 atcgccaccc gcatggtcgc ggcgatcgcc gatgtgcgcg gttacgacgt caccgatccg
      421 cagatccgca ccgcagtcct gctcaccctc gtcggcgtcg atgccaatga ggtggtcaag
      481 aaggcgggag cgattcctgg ggtgtcgttc ggcccgggcg gcaccctcac cgccctcgcg
      541 tcccagcgcc tgcctggccc cgccctcatg gtgctcaaca aggcgatcgg cttccggttg
      601 gtcgcgcagg ccggtcgcac gctgctgggc cgcctcggta aggcggtgcc cgtcgtcggc
      661 ggtgccatcg gcgcgggggt cgacggctat ctgctgagcc ggatcgccga cggcgccacc
      721 cgcgagttcc cgcgccgcgc cgactgagcc ccgtcacggg tcggcggaat ctccggccgg
      781 tcccggtcgt tgaggtgacc gatgggtcgt tcgacccgtc acaggctcag tccgcgagga
      841 gtattgagga catggctgct gcacgggggg cgagctcggc tcgctggggg atgattcggg
      901 ccttggggct cgcactacgg gccgcgaccc ggccgggtgc gccggggatg ggtgaacggc
      961 tctcggcgct gccacgcatg gtgcgcgcgg cgatgcgcgg cgagtatgcc ggtgtcaccc
     1021 gagggcgact gctggggatg ctgggggcgc tgctctacgt cgtgtcgccc gtcgatctcg
     1081 tccctgagag cctcttcacg atcttcggcc tggccgatga cgccctcctg gtcacctggc
     1141 tggcctcgtc gctcgtcaac gacacggagg cgttcctcac ctgggagcgt gggttgtcgc
     1201 gcgccggggc gacctcggcg gccggtggcc cgcaggatgc ccccgccggc gagcgcacca
     1261 ctccggaaga ctcgtcggca cactggaccg ttcagtcgca cgtcgtgcgc tgaggtccgc
     1321 cctgttgtct tcgtgcgctg acgtcgcgcg ctgacgtcgt gggctgacgt cgccgggcgg
     1381 gtgcgggcca agcggttgtc cacacccccc tcgaccgtcg tcacatcgtc cacagccatt
     1441 cgggtgaccc cgctgtctgg gtgcgaacgc ggccacgatc gtcctcatga cgacgacgat
     1501 tcgcatcacc gacccggtcg acctcgtgaa ggtcgtcccg tatcagctgg gttatcaccc
     1561 ggagcgttcc ctcgtgctga tcggcttgcg ccgcaagcag atcgggctcg tgcaacgcct
     1621 cgatctgccg acgctgccgc gtgactgcga ggcagccgcg gacctcatgg ccgggcatct
     1681 gcgcaaggac ggctgcaccg gcgccgtcct tgtgctttac gaggacaccg aaggcgaggg
     1741 tgcagtcgcc gccgctgccc tggtggcccg actgcaggtg gggcgcttcg acctcgtgga
     1801 acacgtggtc gtccgggacg gccacgtgta cttcggggcg ctggccgggc gcgccgaccg
     1861 gccagtgagc ggcgtcctcc ttccgccgga tgaccaggtg ccggccgtcg cggactacgt
     1921 cgcccacggg cgccgacccg ccccctcgcg cggttctctc gatgagcgac tggctcccga
     1981 agacgacgca ttgagcgagc gggtccgcct cgccgcagcc cggctggggg tgctgcggcc
     2041 cgtcctcggc agccggccga aagcgatagc cgactggggc gcattcctcg acgtggggga
     2101 tgacgcctgg ttcgccggga cgcttccatc ggcggcggcc accgcccgga tgtgtcacag
     2161 cctcagggat ctgcaccttc gtgatctggt cacggcctgg ctctgccccg gaacccttga
     2221 gttgaccgcc ttcgacgagg acctgcgagc gcttgcggtc gagcacctgc ccgccgggcg
     2281 cgcctggccg gaggcgtacg cggccgcagg ccacagcccc gcggccggac tggtcgccga
     2341 tgaggcaccc gctgcccgac cgcgcggtgg cgccgggtct cggcgacggc gagccgcctc
     2401 gtcgaagcct cggcgggtcg tcacggcagt ggcgggcccc cggaccgacg aggacgaccg
     2461 gctggtcgag aggctgtgct ggctggcgcg gcatacccct gaggtccatg cgagcgcggt
     2521 gctgagcgtc ctcgcgtccg tgacctggca cctgggtgac ggcaccctca cgcgggtcgc
     2581 cctcgaccgg gcgctcgccc tggaccccgg ctatcggctg gccctgctca tcgagcggat
     2641 ggtcgacctc gccatccgac caggccgcgc cctctcgtcc acgggatgaa ccggtcgtgg
     2701 caggccgccg cggcggcggc atagactcgg gacaggtcat gagtgtcagc gacaagcccc
     2761 ggctcgctga ccggcaaccc ttcttcg
//
LOCUS       DAORPW010000270         2731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_13249_length_2731_cov_408.152840, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000270 DAORPW010000000
VERSION     DAORPW010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13249_length_2731_cov_408.152840"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2054
                     /locus_tag="PLB86_13120"
     CDS             <1..2054
                     /locus_tag="PLB86_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRC66132.1"
                     /translation="LALLRILFSRLGQPHIGSAQAFSFNVASISGAGAVTVQRGGQAV
                     KERREFSITGGMCPRCEGRGAVTDFDLTALYDDSKSLREGALTIPGYTMDGWYGRIFS
                     GSGFFDMDKPIRDYTKRELADLLHKEPTKIKVEGINLTYEGIIPKIQKSFLAKDVEAM
                     QPHIRAFVERAVTFQTCPECAGTRLNEGARSSKIAGINIADACRMQISDLADWVATLD
                     EPSVAPLLATLRHTLDSFVEIGLGYLSLERPAGTLSGGEAQRTKMIRHLGSALTDVTY
                     VFDEPTAGLHPHDIQRMNELLIRLRDKGNTVLVVEHKPEVIEIADHIVDLGPGAGTAG
                     GEVVFEGSVAALRASDTVTGRHLDDRSRLKDSVRMPSGSLPVRGATANNLREVDVDIP
                     LGVLVVLTGVAGSGKSSLVDGSVSGLDGVVSIDQAPIRGSRRSNPATYTGMLEPIRKA
                     FAKANGVKPALFSSNSEGACPACNGAGVIFTDLAMMAGVATTCEECEGRRFQAAVLDY
                     RLGGLNIAEVLDLPVAQAAEFFGPGSGTGSDASSGGAAIPPAAAILGRLADVGLGYVR
                     LGQPLTTLSGGERQRLKLASHMGEKGQIYVLDEPTSGLHLADVQQLLGLLDRLVDSGK
                     SVVVVEHHQAVMAHADWIIDLGPGAGHDGGRIVFEGTPADLVAAKSTLTGTHLAAYIK
                     A"
     gene            complement(2178..>2731)
                     /locus_tag="PLB86_13125"
     CDS             complement(2178..>2731)
                     /locus_tag="PLB86_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRC66133.1"
                     /translation="QGDTVTYTFKVTNTGNVTLAPVTITDTKLNLTDWSCLATLAPGA
                     TANCTSTASYIIQAEDVLNKGIVNTATATGTPPTTPSGAPVIPPATDVSDTGTYPTTP
                     GHVSTVVNPDTNETTIPTSITTTVTNSPTDPTSDPTVLSLAPYSLPRTGGVGVNLYTS
                     GGALIVVGGLAWFLLGLRRRRTA"
BASE COUNT          425 a          917 c          952 g          437 t
ORIGIN      
        1 ccctcgcgct gctgcggatc ctcttcagcc ggctcgggca gccgcatatc ggctcggcac
       61 aagccttttc gttcaacgtg gcgtcgatca gcggcgcagg cgcagtgacc gtccaacgcg
      121 gcggccaggc cgtcaaggag cggcgcgagt tctcgatcac cggcgggatg tgcccgcgtt
      181 gcgaaggccg cggcgcggtg accgacttcg acctcaccgc gctctatgac gacagcaagt
      241 cgctgcgcga gggcgcgttg acgatccccg gctacaccat ggacgggtgg tacggccgga
      301 tcttctccgg cagcgggttc ttcgacatgg acaagccgat ccgcgactac accaaacgcg
      361 agttagccga cctgctgcac aaggagccca ccaagatcaa ggtcgagggc atcaacctca
      421 cctacgaagg catcatcccc aagatccaga agtctttcct cgccaaagac gttgaggcga
      481 tgcagccgca catccgggcc ttcgtcgagc gggcggtcac cttccagacc tgccccgagt
      541 gtgccgggac ccgcctcaat gagggcgcgc gctcgtcgaa gatcgccggc atcaacatcg
      601 ccgacgcctg ccggatgcag atcagcgacc tcgccgactg ggtggcgacc ctcgacgagc
      661 cgtcggtcgc gccgctgctg gcgaccctgc ggcataccct cgactcgttt gtcgagatcg
      721 ggctcggcta cctcagcctt gagcggccgg cgggcaccct ctccggcggc gaggcgcagc
      781 ggaccaagat gatccggcac ctggggtcgg cgttgaccga cgtcacctac gtcttcgacg
      841 agccgaccgc gggcctgcac ccgcacgaca tccagcggat gaacgaactg ctgatccggt
      901 tgcgggacaa gggaaacacc gtcctcgtcg tcgagcacaa gcccgaagtc atcgagatcg
      961 ccgaccacat cgtcgacctc ggtccgggcg cggggaccgc gggcggcgag gtggtcttcg
     1021 aggggtcggt cgcggcgctg cgggcgagtg acacggtcac cgggaggcac ctggacgacc
     1081 gctcgcggct caaagactcg gtgcgtatgc cgtccggctc gcttcccgtg cgcggcgcca
     1141 cggccaacaa cctgcgcgag gtcgacgtcg acatcccgtt gggggtgctc gtcgtcctga
     1201 ccggtgtcgc cgggtcgggc aagagctcgc tcgtcgacgg gtcggtgtcc gggctcgacg
     1261 gggtggtgtc gatcgaccag gcacccatcc gtgggtcgcg gcgcagcaac ccggcgacct
     1321 ataccgggat gctcgagccg atccgcaagg cgttcgccaa ggcgaacggc gtgaaacccg
     1381 ctctgttcag ctcgaactcg gagggcgcct gcccggcctg caacggcgcc ggggtcatct
     1441 tcaccgacct ggcgatgatg gcaggcgtcg cgacgacctg cgaggagtgc gaagggcggc
     1501 ggttccaggc agcggtgctg gactaccggc tcggtgggct caacatcgcg gaggtcctcg
     1561 acctgccggt ggcccaggcg gccgagttct tcggccctgg ctccggtacg gggtctgatg
     1621 ccagctccgg tggggccgcc attccgcccg ccgcggcgat cctcggccgg ttggccgacg
     1681 tcgggttggg ctatgtccgg ctcggtcagc ccctcaccac gctctcgggc ggcgagcgtc
     1741 agcgactcaa gctcgccagc cacatgggcg agaagggcca gatctacgtg ctcgacgagc
     1801 cgacgtcggg tttgcacctg gccgatgtcc agcagttgct cgggttactc gaccggctcg
     1861 tcgactcggg caagtcagtc gtcgtcgtcg aacaccacca agcggtgatg gcgcacgccg
     1921 actggatcat cgaccttggg cccggcgcgg gccatgacgg cgggcggatc gtcttcgagg
     1981 gcacgcctgc cgacctggtc gccgcgaagt ccaccctcac gggaacgcat ctcgcggcat
     2041 acatcaaggc ctaactgcga gcccgccctc cccacgcggc agagcgggcg aagtgcccgc
     2101 agaaacggac cgacgcgcgg cataccttgt cgctctccct ggtatgccgc gcgccggcgt
     2161 ccgagcgcgt tgtccggtca ggcggtacgg cggcgacgca ggcccagcag gaaccaggcc
     2221 aatccgccca cgacgatcaa cgcgccaccc gaggtgtaga ggttcacccc gacgccaccg
     2281 gtgcgcggca gcgagtaggg cgccaacgag agcaccgtgg ggtcgctcgt cggatcggtc
     2341 gggctgttgg tcaccgtggt cgtgatgctg gtcgggatcg tggtctcgtt ggtgtccggg
     2401 ttgaccaccg tgctcacgtg cccaggggtg gtcgggtagg tcccggtgtc ggacacgtcc
     2461 gtggccggcg ggatcaccgg ggcgccggaa ggcgtcgtcg gcggggtgcc ggtggcggtc
     2521 gccgtgttga cgatgccctt attgaggacg tcctcggcct ggatgatgta gctcgcggtc
     2581 gacgtgcagt tcgccgttgc accgggagcc aacgtggcaa gacaggacca gtcggtcaga
     2641 ttcagcttcg tgtcggtgat cgtcacgggc gcgagggtga cgttgccggt gttcgtcacc
     2701 ttgaaggtgt aggtcaccgt gtcgccctgg c
//
LOCUS       DAORPW010000271         2716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_13368_length_2716_cov_593.665539, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000271 DAORPW010000000
VERSION     DAORPW010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13368_length_2716_cov_593.665539"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..956
                     /locus_tag="PLB86_13130"
     CDS             192..956
                     /locus_tag="PLB86_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66134.1"
                     /translation="MSSRQLDRSVRAQLRTLSQENAEGVGAHLVMVAQLLDSNIQAAE
                     AHAETAVRRAGRVPAAREALGLVAYRKGDWARALNEFRTVRRLSGSSHLLPLMVDCER
                     ALGRPDRALDLAASPEVGSLDQAERIELAIVVSGIRRDAGQFESAAAGLETPTLTSAA
                     RRPWTARLRYAYAEAKLALGDVDAARLWFARAAEADDDLATDAAERLEELDGIAYTDL
                     LEGDEGGDPDADVSDDVRDDEGQGPTDSAGRFGGRR"
     gene            970..2055
                     /locus_tag="PLB86_13135"
     CDS             970..2055
                     /locus_tag="PLB86_13135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HRC66135.1"
                     /translation="MGGASGQPSLTAAYLGVICDLDGVVYRGPDAIPYAVSALNDVSR
                     LRPLCFATNNASRTPGEVATQLRSLGLTVEDAAVVTSAQAGAQLLAAELPSGSAVLAV
                     GGPGVPAALAAVGLVVTREPEEARAVLQGWGRDVRVDDLARAAVAIAHGARWVATNTD
                     RTLPTPQGLVPGNGTLVSAVAEATGREPEVVGKPHAPLYLAAAERLAAAPERLLAVGD
                     RLDTDIIGATAVGADSLWVLGGVDGFLSLVGSPARPTYAAHDLRALLDPPVGVERLDP
                     ASWACGDVRLSIVSGRVSAAADGVTIDMSAVPGNQLAALAATVVLDLRGQSASYDLAV
                     SLATEFDRVLGHSAGVNRHRVEDTRPQ"
     gene            2087..>2716
                     /locus_tag="PLB86_13140"
     CDS             2087..>2716
                     /locus_tag="PLB86_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66136.1"
                     /translation="MSFDGIRGYVQFATGIGDATRARAAEVAQGLVTVLSRTSPSEMA
                     GQVSTLAEELLSAAKSNRESLIHLIRGEVSELIESSSSLAKVADLDSMRAALTQLGSD
                     LDGLRQQVGVQSPAALVSSGAAAVVTAASSVRPGGSRPAPKPAARIDVPAKVKRVPSR
                     PRGSAASTANDAPVVAPQASAVEGPVVRKVSPVSAPEAPSTPEVRKLTAK"
BASE COUNT          381 a          886 c          978 g          471 t
ORIGIN      
        1 ctcgacggga cgactcgtct cagcggggcg gcactgaccg tggtccgcga cgagatggcc
       61 aagggccagc gcgctccggt ggtcatgctc ctcgtgccgg cgctggtcgc ccggacgggg
      121 aggctccccg gggtggggct cggctagcgc ctaagtcggt tcgccttccg gagccggcta
      181 ttcccgaggg tgtgagcagt cgtcaactgg atcgttcggt tcgcgcccag ctgcgcacgc
      241 tcagccagga gaacgccgaa ggtgtgggcg ctcacctggt gatggtggcg caattgctcg
      301 atagcaacat tcaagctgcg gaggcgcatg ccgagaccgc ggttcgtcgc gccggtcggg
      361 ttcccgccgc acgagaggct ttgggcctgg tcgcctatcg caagggcgat tgggcgcgtg
      421 ccctcaacga attccggacc gtccgaaggt tgtcgggctc atcccatctg ttgccgttga
      481 tggtcgattg cgagcgagct ctgggccggc cagatcgggc tttggatctc gctgcatccc
      541 cggaggtcgg atcgctggat caggccgagc gcatcgagtt ggcgatcgtc gtgtctggca
      601 tccgtcggga cgccggccag ttcgagtcgg cggcagcggg tctggagacc cccacgttga
      661 ccagtgccgc gcgacgaccg tggaccgcgc ggctgcgtta tgcgtatgcc gaggccaaac
      721 tcgccttggg cgacgtcgat gctgcgcgac tgtggttcgc ccgcgcagcc gaggctgacg
      781 acgatctggc caccgacgct gccgagcggc tcgaggagct tgacgggatc gcctacacgg
      841 atctcttaga aggcgacgag ggcggcgacc ccgacgcgga cgtgtccgac gatgtcaggg
      901 acgatgaggg ccaaggcccc acggattcgg ccggccggtt cggtggccgg cgttagtcac
      961 gatggggccg tgggaggggc gagcggccaa ccgtcgctga cggcggcata cctcggcgtc
     1021 atctgcgacc tcgatggggt ggtttatcgg ggaccggacg cgatcccgta tgccgtctcg
     1081 gcactgaacg acgtgtctcg cctgcgtcca ctgtgttttg ccaccaacaa tgcgtcccgg
     1141 actccggggg aggtggcgac ccagttgcgg agcctcggcc tgaccgtcga ggacgctgcc
     1201 gtggtcacta gtgcccaggc cggcgcgcag cttctcgcgg ctgaacttcc ctctggtagc
     1261 gcggtcctcg ccgtcggtgg gccaggggtg ccagcagcct tggccgctgt cggactggtg
     1321 gtgacccggg agcctgaaga ggcccgcgcg gtgctgcagg gctggggacg tgacgttcgt
     1381 gtcgatgacc ttgccagggc cgccgtggcg atcgcccatg gcgctcgctg ggtggcgacc
     1441 aacaccgatc gcacgcttcc cacccctcaa gggctggttc ccggcaacgg cacgctggtg
     1501 tccgcggtcg ctgaggccac gggtcgcgaa ccggaagtgg tcggcaagcc gcatgcgccc
     1561 ctctacctcg ccgcggccga gcgcttggcc gctgcccctg aacggctcct cgcggtcggc
     1621 gacaggctcg acacagacat catcggcgcg accgcggtcg gcgccgattc gctgtgggtc
     1681 ctcgggggcg tcgacgggtt cctctcactg gtcggttcac cagcacgacc cacgtatgcc
     1741 gcgcacgacc tgcgcgcgct ccttgacccg ccggtcgggg tcgagcgact cgacccggcg
     1801 tcgtgggcgt gcggcgacgt ccgcctgtcg attgtctccg ggagggtgtc ggcggcggcg
     1861 gatggcgtca ccatcgatat gagtgctgtg cctggtaacc agttggccgc gctcgcagcg
     1921 actgtggtgc tcgatctgcg cggccaaagc gcgtcatacg acctggccgt gagtctcgcc
     1981 actgagtttg atcgtgttct cggccatagc gccggggtta accgccatcg ggtcgaggac
     2041 actcgacccc agtagcgtgt gggtacaccc atcgaggagg agcgacatgt cgtttgacgg
     2101 catccgtggt tatgtccagt tcgcgaccgg catcggtgac gcgaccagag cgcgtgccgc
     2161 tgaggtggcg caaggtctgg tgacggtcct gagcaggacg tcgccgtcgg agatggcggg
     2221 tcaggtgtcg accctggccg aagaactcct cagtgcggca aagtccaatc gggagagcct
     2281 cattcacctc attcgtggtg aggtgagcga gctcatcgag tcgtcctcgt cgctggccaa
     2341 ggtcgccgac ctcgactcca tgcgggccgc cctgactcag ctcggaagcg acctcgacgg
     2401 gctgcggcag caggtgggag tgcagtctcc ggccgcgctg gtgtcctccg gtgccgccgc
     2461 ggtcgtcacc gccgcatcga gcgttcgacc gggtggttcg cgtccggctc cgaagcccgc
     2521 cgcacgcatc gatgttccgg ccaaggtgaa gcgagttccg tcccggccgc gcgggtcggc
     2581 tgccagcact gcgaacgacg caccggtcgt cgccccgcag gcgtcagccg tcgaggggcc
     2641 ggttgtccgc aaggtttcgc cagtgtcggc ccccgaggct ccgagcaccc ctgaggttcg
     2701 caagctgacg gcgaag
//
LOCUS       DAORPW010000272         2703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_13483_length_2703_cov_577.424849, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000272 DAORPW010000000
VERSION     DAORPW010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13483_length_2703_cov_577.424849"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..636
                     /gene="frr"
                     /locus_tag="PLB86_13145"
     CDS             79..636
                     /gene="frr"
                     /locus_tag="PLB86_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRC66137.1"
                     /translation="MIDETLFEAEEKMEKAVEVAKEDFAGIRTGRANPGMFNKLLVDY
                     YGSPTPMQQLASFQTPEARTIIIKPFDAKAMHGIERAIRDSDLGVNPSNDGVVIRCIL
                     PVLTEERRRDYIKLAHHKAEEARVSIRNVRRKAKTDLEHLIKDGEVGEDDGNRAEKEL
                     DQMTKKYVDTIDHLVKGKEAELLEV"
     gene            677..1519
                     /locus_tag="PLB86_13150"
     CDS             677..1519
                     /locus_tag="PLB86_13150"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRC66138.1"
                     /translation="MSEAPLASPSSRAGRNLPAAIGVGLLLGALIIGTHVWRKELFLV
                     LATASVSVGCWEIAKAMQRKGIRAPVFPVVLGCVVQMTGAYFLGEQALFVAFVLTAAL
                     IVMWRGSESISGAATDVSAGVFIAAYPALLAGFAMLLLAAPDGAWRSFTFLCVTVASD
                     VGGYAVGVLAGRHPMAPRISPKKTWEGFAGSFLGCILVGIICVTVGLHGPWWVGAILG
                     AVTAPAATLGDLVESAIKRDLGIKDMSNILPGHGGLMDRLDSLMVSAPFIWATLTILL
                     PLPK"
     gene            1640..>2703
                     /gene="rlmN"
                     /locus_tag="PLB86_13155"
     CDS             1640..>2703
                     /gene="rlmN"
                     /locus_tag="PLB86_13155"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRC66139.1"
                     /translation="MPPRHLADLDAAGRKAAVEALGHKGFRAGQLSKHYFERLVDAPE
                     EMTDLPGAARAELVDALLPRLLTPVKHLVADEGRTVKSVWRLHDGALVESVLMRYPER
                     VTMCISSQAGCGMNCPFCATGQGGLTRNLSTAEIVEQVVAGARMLRRGEVPGGDADAE
                     RERPLRVSNVVFMGMGEALANYSRAIAAIRRLTDPAPDGLGMSARGLTMSTVGLVPAI
                     DRLAGEGIPVTLALSLHAPDDELRDELVPINTRWRVDEALDAAYRYFEATGRRVSIEY
                     ALIRDINDQSWRADLLGEKLARRGRGWVHVNPIPLNPTPGSKWTASRTGVQQQFVERL
                     RAHGIPTTVRDTRGREIDGA"
BASE COUNT          433 a          969 c          891 g          410 t
ORIGIN      
        1 cccgtgccct gcgaggtgag aggatcggca cactggtgac cgccggatga cccacatgca
       61 aggagcagga cgaccgcgat gatcgacgag acactcttcg aggccgaaga aaagatggaa
      121 aaggccgtcg aggttgccaa ggaagacttc gccggcatcc gcaccggacg ggccaacccg
      181 gggatgttca acaagctcct cgttgactac tacgggtcgc cgacgccgat gcagcagttg
      241 gcgtccttcc agacccctga ggcgcggaca atcatcatca agccgttcga cgcgaaggcg
      301 atgcacggca tcgagcgcgc gatccgcgac tccgacctcg gggtcaaccc cagcaacgac
      361 ggcgtcgtca tccggtgcat cctcccggtc ctcaccgagg agcgccgccg cgactacatc
      421 aagctcgccc accacaaggc cgaggaagcc cgggtgtcga tccgcaacgt ccggcgcaag
      481 gccaagaccg acctcgagca cctcatcaag gacggcgagg tcggcgagga cgacggcaac
      541 cgcgccgaga aggaactcga ccagatgacc aagaagtacg tcgacacgat cgaccacctg
      601 gtcaagggca aggaggccga gctgctcgag gtgtgaagcc tcgaccctcg gcgtcccgat
      661 gagcgcccct cagcccatgt ccgaggcccc cttggcctcc ccgtcctcac gtgccgggcg
      721 caacctcccg gccgcgatcg gtgtcggact gttgctgggt gccctcatca tcggcaccca
      781 cgtctggcgt aaggaactct tcctcgtcct cgcaacggca tcggtctccg tcggctgctg
      841 ggagatcgcc aaagcgatgc agcgcaaggg gatccgggcg cccgtcttcc ccgtggtcct
      901 cggctgcgtc gttcagatga cgggtgccta cttcctcggc gagcaggcgt tgtttgtcgc
      961 cttcgtgctc accgcggccc tgatcgtcat gtggcgcggc agcgagagca tcagcggcgc
     1021 ggcgaccgac gtcagcgcgg gcgtgttcat cgcggcatac cccgcgttgc tggcgggctt
     1081 cgcgatgctc ctgctcgccg ctccggatgg cgcgtggcgt agcttcacct tcctgtgcgt
     1141 gaccgtcgcg agcgacgtcg gagggtatgc cgtcggcgtc ctcgccgggc gccacccgat
     1201 ggcgccgcgg atcagcccga agaagacctg ggaaggcttc gccggctcgt tcctcggctg
     1261 catcctcgtc ggcatcatct gcgtcaccgt cggcctgcac gggccgtggt gggtcggcgc
     1321 gatcctcggg gcggtcaccg cgcccgccgc cacgctgggc gacctggtcg agtcggcgat
     1381 caagcgtgac ctcgggatca aggacatgtc caacatcctg cccggccacg gcggcctgat
     1441 ggaccggctc gactcgctga tggtctcggc gccgttcatc tgggccacgc tgaccatcct
     1501 ccttccgctg cccaagtgac tcggtgatcc ggtgaccaac ccgcccgaag cgaccggcgt
     1561 tccttccgcc gcgcccgcac cccgtgccac gcggcggccc gcccccggcg agctcacctt
     1621 cgaagccccc cggcgcggta tgccgccgcg tcacctcgct gacctcgacg cagccgggcg
     1681 caaggcagcc gtcgaagcct tgggccacaa gggattccgg gccggccaac tgtccaagca
     1741 ctacttcgag cggctcgtcg acgcgcctga ggagatgacc gacctgcccg gcgccgcccg
     1801 cgccgagctc gtcgacgctc tcctgccgcg gctgctcacc ccggtcaagc acctcgtcgc
     1861 ggatgaaggc cgcaccgtca agtcggtgtg gcgactgcat gacggcgccc tcgtcgaatc
     1921 ggtgctcatg cgttaccccg agcgggtgac gatgtgcatc tccagccagg ccggctgcgg
     1981 gatgaactgc cccttctgtg cgaccgggca gggcgggttg acccgcaacc tgtcgacggc
     2041 cgagatcgtc gagcaggtcg tcgccggagc caggatgctg cgtcgaggtg aagtgcccgg
     2101 tggcgacgcc gacgccgagc gcgagcgtcc cctgcgggtg tcgaacgtcg tcttcatggg
     2161 gatgggggag gccctggcca actactcccg tgccatcgcc gcgatccgcc ggctcaccga
     2221 cccggcgccc gatgggctgg ggatgtcggc tcgcgggctg acgatgtcga cggtcgggct
     2281 cgtgccggcc atcgaccgac tggccggcga gggcatcccc gtcaccttgg cgctgtcact
     2341 gcatgccccc gacgacgagt tgcgcgacga actcgtgccc atcaacacgc ggtggcgggt
     2401 cgatgaggcg ctggacgcgg cataccgcta tttcgaggcg accggccgac gcgtctccat
     2461 cgaatacgcc ctcatccgtg acatcaacga ccagtcctgg cgcgcagacc tgttggggga
     2521 gaagctcgcc cgccgcggcc gcgggtgggt gcacgtcaac cccatcccgc tcaaccccac
     2581 gcccggctca aaatggacgg cgtcgcggac cggagtccag cagcagttcg tcgagcggct
     2641 gcgggcacac ggcataccga cgacggtccg cgacacccgc gggcgcgaga tcgacggtgc
     2701 ctg
//
LOCUS       DAORPW010000273         2700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_13498_length_2700_cov_655.280151, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000273 DAORPW010000000
VERSION     DAORPW010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13498_length_2700_cov_655.280151"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLB86_13160"
     CDS             complement(<1..223)
                     /locus_tag="PLB86_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRC66140.1"
                     /translation="MAGNSQRRGAVRKSGKGPTVGSGGQRRRGLEGKGPTPKAADREK
                     HIAHKRSQRPADSGQGAGFGGSGSGGRYAA"
     gene            complement(225..1691)
                     /gene="cysS"
                     /locus_tag="PLB86_13165"
     CDS             complement(225..1691)
                     /gene="cysS"
                     /locus_tag="PLB86_13165"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRC66141.1"
                     /translation="MSLHLFDTATRAMREFRPLEDGKVGIYICGLTTQGAPHIGHVRF
                     AVAFDVLRRWLTIGHGLDVTLVRNVTDIDDKILAKATEAGQEWWAWSYRNERLTSEAL
                     DLVGVLPATYEPRATGHILEMVELMDTLVEKGHAYAAADGSGDVYFDVKSWPEYGSLT
                     GQALDDMAPAEDADPRGKHDPRDFALWKGHKSGEPDSAQWPTHFGAGRPGWHLECSAM
                     ARRYLGDAFDIHGGGIDLRFPHHENEQAQSRAAGLPFANFWLHNAWVTVRDQKMGKSL
                     GNALEVQVVTQVARPLAVRYYLTAAHYRSMIEYHEGSLAEAGAAVDRIEGLLERVDQV
                     RPLADRVGVLPPAFREAMDDDLNVSGALAVVHDTVRAANTALDDGDLDGAAELAAAVV
                     AMTDILGVNPLDPHWAGATTSGQGDATSALDALVQAQLAARQSARAARDFAAADAIRD
                     QLTTAGIAIEDTPSGPRWSLARHPDAAPGAPSVPQPKD"
     gene            complement(1875..2549)
                     /locus_tag="PLB86_13170"
     CDS             complement(1875..2549)
                     /locus_tag="PLB86_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC66142.1"
                     /translation="MPPTLADVVAALPRSAAGVRVEHLVTSGTFSLDGGTWDVDNNVW
                     ILGDDTQCVVIDAPHDAGPILAAIGDRRLLAVLCTHAHDDHVTAALEVCGATGATAYL
                     HPADRVLWDRVHASAPDHDLADGDVLQVGDVEVQVLHTPGHAPGACCFSVPALGVLFS
                     GDTLFNGGPGATGRSFSDFPTIVESIEQRLLSLPADTVVLTGHGDATTIGAEQPHLGE
                     WLARGH"
     gene            complement(2555..>2700)
                     /locus_tag="PLB86_13175"
     CDS             complement(2555..>2700)
                     /locus_tag="PLB86_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HRC66143.1"
                     /translation="LYRQGRFPLEDFVSERIGLGDVEAAFEKMHHGEVLRSVVEIAGG
                     PSA"
BASE COUNT          396 a          914 c          991 g          399 t
ORIGIN      
        1 gggcggcata cctgccgccg gaaccggagc ccccgaaacc ggcaccctgc cccgagtcgg
       61 ccgggcgttg gctgcgcttg tgggcgatgt gcttctcgcg gtcggcggcc ttgggggtcg
      121 ggcccttacc ttcaaggccg cgacggcgtt ggcctcccga gcccacggtg ggccccttgc
      181 cgctcttgcg gaccgccccg cggcgctggg agttgccggc catgtcagtc cttcggttgg
      241 ggaacggacg gggcgccggg cgccgcgtcg gggtgccggg ccagcgacca acgcgggccg
      301 gacggggtgt cttcgatggc gatccctgcg gtggtgagct gatcgcgaat ggcatcagcg
      361 gcggcgaaat cccttgcagc ccgggctgat tgacgggcag cgagctgtgc ctggacgagt
      421 gcgtcgagcg cactcgttgc gtcgccctgg ccggatgtcg tcgcccccgc ccaatgcggg
      481 tcgagcgggt tgacgccgag gatgtcggtc atggcgacga ccgccgcagc aagctcggcg
      541 gccccatcga ggtcgccgtc gtccagagcg gtgttggccg cccggacggt gtcgtggacg
      601 accgcgaggg cgcctgagac attgagatcg tcgtccatcg cctcgcggaa cgcgggcggc
      661 agcaccccga cgcggtcggc gagcgggcgc acctggtcga cccgctcgag caaaccctcg
      721 atccgatcga ccgcggcacc ggcctcggcc aacgacccct cgtgatattc gatcatcgag
      781 cggtagtgcg ccgccgtgag gtagtagcgg actgcgaggg ggcgggccac ctgagtgacg
      841 acctggacct ccaaggcgtt gccgagcgac ttgcccatct tctggtcgcg caccgtcacc
      901 caggcgttgt gcaaccagaa gttggcgaac ggcagtccgg ccgcgcgcga ttgggcctgc
      961 tcgttctcat ggtgggggaa gcgcaggtcg atgccgccgc cgtggatgtc gaacgcatcg
     1021 ccgaggtagc gccgggccat cgccgagcac tccaggtgcc agcccggtcg gccggcgccg
     1081 aagtgggtcg gccattgggc gctgtcgggc tcgccggact tgtgaccctt ccagagggcg
     1141 aagtcgcggg gatcgtgctt gccgcgcggg tcggcgtcct cggccggggc catgtcatcc
     1201 agcgcttgac cggtcagcga gccgtattcc ggccaggatt tgacgtcgaa gtagacgtcg
     1261 ccgctgccgt cggcggcggc atacgcatgg cccttctcga ccagcgtgtc catgagctcg
     1321 accatctcga ggatgtgccc ggtcgcgcgc ggctcgtagg tcgcggggag gacgccgacc
     1381 aggtcgagcg cctcgctcgt cagccgctcg ttgcggtagg accaggccca ccactcctgg
     1441 cccgcctcgg tcgccttggc gaggatcttg tcatcgatgt cggtgacgtt gcgcacgagc
     1501 gtgacatcca gaccgtgccc gatggtgagc cagcggcgca gcacgtcgaa cgccaccgcg
     1561 aagcgcacgt ggccgatgtg gggggcgccc tgggtcgtga gcccacagat gtagatcccg
     1621 accttgccgt cctccaaggg gcggaactcg cgcatggcgc gggtggcggt gtcgaacagg
     1681 tgcaggctca cccgggcaag tttagggggt atgccgttcg ccccctcccg aacgcgacgc
     1741 gcaccgctgc cggtgcatcg gggagcggac ggggaggctc ggagatggag gagcggacat
     1801 ggaggcgggg cgggtcaccg tgcccctcaa cccggtgacc cgccccgccg tctgacgagg
     1861 tcgacgccga tgcgtcagtg acctcgagcc aaccactccc ccaggtgcgg ttgttcagcc
     1921 ccgatcgtcg tcgcgtcgcc gtgcccggtg agcacgacgg tgtcggccgg cagtgacaac
     1981 agccgctgct cgatcgactc gacgatggtc gggaaatcac tgaacgagcg gccggtcgcg
     2041 cccgggccgc cgttgaagag ggtgtcgccg gagaagagga cgcccagcgc cggcaccgag
     2101 aagcagcagg cccccggcgc atgacccggc gtgtgcaaca cctggacctc gacgtccccg
     2161 acctgcagca cgtcgccgtc ggccaagtcg tggtcgggcg cgctcgcgtg gacgcggtcc
     2221 cacagcacgc ggtcggcagg atgcaggtat gccgtggcgc cggtcgcgcc acacacctcc
     2281 agggccgccg tgacgtggtc atcgtgggcg tgggtgcaca agaccgcaag cagacgtcga
     2341 tcaccgatcg ccgcgaggat cgggccggcg tcgtgcgggg cgtcgatgac cacgcactgg
     2401 gtgtcgtcac caaggatcca cacgttgttg tcgacatccc acgtcccgcc gtcgaggctg
     2461 aaggtccccg acgtcacgag gtgctcgacc cgcacgccgg ccgccgaacg gggcagggcg
     2521 gcgacgacgt cggcgagggt gggcggcata ccggtcatgc ggaggggccc cccgcgatct
     2581 cgacgaccga gcggaggacc tcgccgtggt gcatcttctc gaaggcggcc tcgacgtcgc
     2641 ccagaccgat ccgctcggag acgaagtcct ccaaggggaa gcgaccctga cggtagagct
//
LOCUS       DAORPW010000274         2692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_13555_length_2692_cov_905.714069, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000274 DAORPW010000000
VERSION     DAORPW010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13555_length_2692_cov_905.714069"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLB86_13180"
     CDS             complement(<1..426)
                     /locus_tag="PLB86_13180"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HRC66144.1"
                     /translation="MLDPAAFDPVPWANGAGTTRELAVARDAEGQTLWRVSLADLDQS
                     ASFSSLPGLDRIFTPLGSVRLTIDGTTVDVAAGEQVRFPGEAVVAVVLSRPTRALNVM
                     TRRGRCVAQVTLRPAEAPARGEATVSVDLGERIAEVRVTI"
     gene            complement(452..721)
                     /locus_tag="PLB86_13185"
     CDS             complement(452..721)
                     /locus_tag="PLB86_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRC66145.1"
                     /translation="MELTPDELAKLANRLRRAQGQIGGVLRMIEEGRDCQDIVTQLAA
                     VRRAVDRVGFSIIATGLKQCVTEAHDRGDNEVDTALMEKLFISLA"
     gene            887..2134
                     /locus_tag="PLB86_13190"
     CDS             887..2134
                     /locus_tag="PLB86_13190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRC66146.1"
                     /translation="MRTLVILGGGTAGTMAANKLRARLAAEEWQIIVIDKDDNHDYQP
                     AYLFVPFGINKVKQVRKSRRSFIDDGIEVRMADIERVDAEARLVHLGDGSELGYDYLI
                     MATGTTPRPDQTPGMLGPEWRKSVHEFYTYDGMLALKKSLKDFQGGRLVMHITELPIK
                     CPVAPLEFVMLADDFLTKKGVRAATEIVYVTPLDGAFTKPVASRELGHHLSTRNIKVE
                     PDFMIESVDQEAKVIRSYDEREVAYDLLVTVPLNMGADYVAASGLGDELNYVPVDKHT
                     NQSTAYPNIFALGDGSNLPTSKAGSVAHFSVEVFVDNFCELIEGRPMTHSFDGHANCF
                     VESGNGRALLLDFNYETEPLTGHFPLPVGPMSLLKATRANHVGKLAFRHVYWNVLLPG
                     RPLPLHAEMSMLGKRVEPQPVDA"
     gene            2214..2528
                     /locus_tag="PLB86_13195"
     CDS             2214..2528
                     /locus_tag="PLB86_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HRC66147.1"
                     /translation="MPTTTIAGRQIDVDAEGFLTQPDQWDEDLALTLAANIGITLTDA
                     HWVPIHFLRKDFETQKETATLRRVSTVGGIPVKELFVLFPGKPAKKMSYIAGLQKPKG
                     CV"
BASE COUNT          495 a          965 c          820 g          412 t
ORIGIN      
        1 gatggtgacg cggacctcgg cgatgcgctc gccgaggtcg acgctcaccg tcgcctcgcc
       61 acgtgctgga gcctcggccg gccggagggt gacctgggcg acgcacctgc cgcggcgcgt
      121 catcacgtta agggctcggg tcgggcgaga cagcacgacc gcgaccacgg cttcaccggg
      181 gaaccgaacc tgctctccgg cggcgacgtc gacggtggtg ccgtcgatcg tcaggcggac
      241 cgacccgagc ggggtgaaga tccggtcgag gcccggcaac gaggagaacg acgcgctctg
      301 atcgaggtcc gcgaggctga cccgccacag ggtttggccc tcggcgtcgc gagccacggc
      361 cagctcccga gtggtcccgg caccgttggc ccagggaaca gggtcgaacg ccgccggatc
      421 cagcatcacc gcaggctccg cgtcgtcacg gctaggcgag ggagatgaag agcttctcca
      481 tgagggcggt gtcgacctcg ttgtcgcccc gatcgtgggc ctcggtcaca cactgcttga
      541 ggccggtggc gatgatcgag aagccgaccc ggtcgaccgc tcggcggacc gcggccagct
      601 gcgtgacgat gtcctggcag tcgcgaccct cttcgatcat ccgcagcaca cccccgatct
      661 gaccctgggc gcggcgcaac cggttggcca gcttcgccag ctcgtcaggc gtcagttcca
      721 cctttgtcat acccgtcatc ctcccacgac tccttcggct gatcgggggg ttcgctgtgc
      781 tgcacggcac acaggggagg accttcggcc ccgctggaca gcatacccgc gggggtattc
      841 tctcatgtat caggttactc cgcgagaact acataaggac caagcaatgc gcaccctcgt
      901 gatcctcggc gggggaaccg ccggaaccat ggcagccaac aagctgcggg ctcgactggc
      961 ggccgaggaa tggcagatca tcgtcatcga caaggacgac aaccacgact accagccggc
     1021 ctacctgttc gtgccgttcg gcatcaacaa ggtcaagcag gtgcgcaagt cccgccggtc
     1081 gttcatcgat gacggcatcg aggtgcggat ggccgacatc gagcgcgtgg acgccgaagc
     1141 ccgactcgtc cacctcggtg acggcagcga gctcggctac gactacctca tcatggccac
     1201 ggggacgacc ccccggccgg atcagacccc cgggatgctc ggccccgagt ggcgcaagag
     1261 cgtccacgag ttctacacgt acgacggcat gttggcgctg aagaagtcac tcaaggactt
     1321 ccagggcggc cggctggtca tgcacatcac cgagctgccc atcaagtgcc ccgtcgcccc
     1381 gctcgagttc gtcatgctcg ccgacgactt cctcaccaag aagggcgtgc gggccgccac
     1441 cgagatcgtc tacgtcaccc cgctggatgg cgccttcacc aagccggtcg ccagccgcga
     1501 gctcggccat cacctgtcca cgcgcaacat caaggtcgag cccgacttca tgatcgagtc
     1561 ggtcgaccag gaggcgaagg tcatccgctc ctatgacgag cgcgaggtgg cctacgacct
     1621 gctggtcacc gtgccgctca acatgggcgc cgactatgtg gctgcctccg gcctgggcga
     1681 cgagctcaac tacgtgcccg tcgacaagca caccaaccag tccacggcat acccgaacat
     1741 cttcgcgctc ggggacggga gcaacctgcc cacgtcgaag gccggctcgg tggcgcactt
     1801 ctcggtcgag gtgttcgtcg acaacttctg cgagctcatc gagggccgcc cgatgaccca
     1861 ctccttcgac gggcatgcca actgcttcgt cgagtcgggc aacggtcggg cgctgctgct
     1921 ggacttcaac tacgagaccg agccgctcac cggacacttc cccctgccgg tcggcccgat
     1981 gagcctgctc aaggccaccc gcgccaacca cgtgggcaag ctggccttcc ggcacgtcta
     2041 ctggaatgtc ctgctccccg gccggccgct gccgttgcac gcggagatgt cgatgctcgg
     2101 caagcgcgtc gagccccagc ccgtcgacgc ctgatccacc gccacacaat cctgccccga
     2161 ctcccatcca cgacccagcc ccaccagcag aaacccaagg agaactgacc cccatgccca
     2221 ccaccacgat cgccggccgt cagatcgacg tcgacgccga gggcttcctc acccagccgg
     2281 atcagtggga cgaggacctc gccctgaccc tcgctgccaa catcggcatc accctgaccg
     2341 acgcccactg ggtgccgatc cacttcttgc gcaaggactt cgagacccag aaggagaccg
     2401 cgacgttgcg tcgcgtgtcg accgtgggcg gcataccggt caaggaactg ttcgtgctct
     2461 tccccgggaa gcccgccaag aagatgtcct acatcgccgg cctgcagaag cccaagggct
     2521 gcgtctgatc gcccagatca gccagttgac tacgaagaga agaagatcac gatgaccgac
     2581 atgatcgtcc cgtcgttcga cgacgagacg ccgaccgggc gcaagctcgc catcatttgc
     2641 tccaagggca acctcgacat ggcctacccc ggcctcatcc tcgccaacgc cg
//
LOCUS       DAORPW010000275         2689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_13587_length_2689_cov_990.187927, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000275 DAORPW010000000
VERSION     DAORPW010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13587_length_2689_cov_990.187927"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PLB86_13200"
     CDS             complement(<1..1084)
                     /locus_tag="PLB86_13200"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRC66148.1"
                     /translation="MSPRPDPSPSPRSGPDLATIIAAPKVLLHDHLDGGVRPQTVIEI
                     AEQIGYADLPSTDVAELDQWFRDSADSGSLVRYLQTFDQTVAVMQTEEGLHRIAKECV
                     LDLATDGVVYAESRYAPEQHLAEGLTLEQVVEAVNAGFRDGEREAAAAGHPIQVTALL
                     TAMRHAAKSSEIARLAVRYRDDGVAGFDIAGAEDGHPPTRHLDAFEYLQRENAHFTIH
                     AGEAFGLPSIWEAIQYCSADRLGHGVRIVDDITIDGVPVPLWVAQAHAAHVNTYDEVD
                     LSTVRLGRLAAYIRDSRIPLEMCPSSNLQTGAARSIGLHPISLLLRLRFRVTLNTDNR
                     LMSGTSMSREAALLVEHAGWTLGDLRW"
     gene            complement(1087..1440)
                     /locus_tag="PLB86_13205"
     CDS             complement(1087..1440)
                     /locus_tag="PLB86_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC66149.1"
                     /translation="MPHVTTAPTRIPVPGGKVIDEHVGAASTGTDAVSVAHMVAPPGW
                     EEPYQTPQFDEITVVVRGSIHVDCDGVRHEVHAGQSIVTTAGERIRYAAGPDGAEYVA
                     VCLPAFTPDRVHRED"
     gene            complement(1499..>2689)
                     /locus_tag="PLB86_13210"
     CDS             complement(1499..>2689)
                     /locus_tag="PLB86_13210"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRC66150.1"
                     /translation="RGVVADEQMSSLAMAILLNGMTRREIARWTGAMIASGERMDFSS
                     LSRPTADKHSTGGVGDMITLPLAPLVAACGVAVPQLSGRGLGHTGGTLDKLESIPGWR
                     ASVSNEQMLRQLEEVGAVICAAGAGLAPADKKLYALRDVTGTVEAIPLIASSIMSKKI
                     AEGTGALVLDVKVGSGAFMKDVAQAQELARTMVALGTDAGVRTVAMLTNMQTPLGLTA
                     GNALEVRQSVEVLAGGGPPDVVDLTLTLAREMLAAAGREEIDPADALADGRAMDVWHR
                     MIQAQGGDSHAPLPWARETHEVRAPGTGVLTQLDALKVGVAAWRLGAGRARKEDPVQA
                     GAGVVMHAKPGAYVRAGDPLLTLHTDTPERFERALEALEGAWFIAPEGSRPDLLPLVI
                     DRIS"
BASE COUNT          379 a          939 c          955 g          416 t
ORIGIN      
        1 cccagcgcag atcgcccaac gtccacccgg cgtgctcgac gagcagcgca gcctcgcgtg
       61 acatcgaggt gccgctcatc aaccggttgt cggtgttgag cgtgacccgg aaccgcaggc
      121 gcaacaacag ggagatcggg tgcaggccga tgctccgcgc ggcgccggtc tgcaggttgc
      181 tgctcgggca catctccagg gggatccggg agtcgcggat gtatgccgcg agcctcccga
      241 gccgcaccgt cgacaggtcg acctcgtcgt aggtgttgac gtgcgcggcg tgcgcctgag
      301 ccacccagag gggcacgggc accccgtcga tggtgatgtc gtcgacgatc cgcaccccgt
      361 ggccgagccg atcagccgag cagtactgga tggcctccca gatgctcggc aacccgaagg
      421 cctcgcccgc gtggatggtg aagtgggcgt tctcgcgctg caggtattcg aacgcgtcaa
      481 ggtggcgggt cggcgggtga ccgtcctcgg ccccggcgat gtcgaatccg gccaccccgt
      541 cgtcgcggta acgcacggcc aaccgggcga tctccgagct cttggccgca tggcgcatcg
      601 ccgtgagcag cgccgtgacc tggatcgggt gaccggctgc cgcagcctcg cgctcgccgt
      661 cccggaagcc ggcgttgacc gcctcgacga cctgctcgag ggtcaggccc tcggccaggt
      721 gctgctcggg ggcatagcga ctctcggcat agacgacgcc gtcggtggcg aggtcgagga
      781 cgcactcctt ggcgatccgg tgcagccctt cctcggtctg catgaccgcc accgtctggt
      841 cgaaggtctg cagatagcgc acgagcgagc ccgagtcggc gctgtcgcgg aaccactggt
      901 ccaactcggc gacgtcggtg ctcggcaggt cggcataccc gatctgttcg gcgatctcga
      961 tgaccgtctg ggggcgcact cccccgtcga ggtggtcgtg caggagcacc ttcggggccg
     1021 cgatgatcgt cgcgagatca ggaccggagc gcgggctcgg gctcgggtca ggccgcgggc
     1081 tcacggtcag tcctctcggt ggacgcggtc gggggtgaag gcgggcaggc agacggcgac
     1141 atactctgcg ccgtcggggc cggcggcata ccggatgcgt tcgcctgcgg tcgtgacgat
     1201 cgactgtccg gcgtggacct cgtgacggac gccgtcgcag tccacgtgga tcgagcccct
     1261 gacgacgacg gtgatttcgt cgaattgtgg tgtctggtaa ggctcttccc acccgggcgg
     1321 ggcgaccatg tgagcgaccg acacggcgtc ggtgccggtc gatgcggctc ccacgtgttc
     1381 gtcgatgacc ttgcccccag ggaccgggat ccgcgtcggc gcggtagtga cgtgcggcat
     1441 tctcgcagcc tagagggttc ccgagccccc gcgtatgccg tgcggcccgg tccgcggatc
     1501 agctgatccg gtcgatgacg aggggcagca ggtcgggacg ggacccttcc ggtgcgatga
     1561 accacgcacc ctccaacgcc tccagcgcgc gctcgaagcg ttcgggggtg tcggtgtgca
     1621 aggtgagcag cgggtcgccg gcccggacgt aggcacccgg tttggcgtgc atgacgacac
     1681 cggccccggc ctgcaccggg tcttccttgc gagcccggcc agcaccgagc cgccaggctg
     1741 ccaccccgac cttgagggcg tcgagttggg tcagcacccc cgtgccgggc gcccgcacct
     1801 catgggtctc ccgcgcccac ggcagcggcg cgtgcgagtc gccgccctgc gcctggatca
     1861 tccggtgcca cacgtccatc gcgcgcccgt ccgccaacgc gtcggccggg tcgatctcct
     1921 cgcggccggc ggctgccagc atctcgcgcg ccagcgtgag ggtcaggtcg acgacgtccg
     1981 gaggaccacc gccggcgagc acctcgaccg actgacgcac ctcgagagcg ttgcccgcgg
     2041 tgaggcccag cggcgtctgc atgttggtga gcatcgcgac cgtcctgact ccggcgtcgg
     2101 tccccagcgc caccatggtc cgggccagtt cctgggcctg ggctacgtcc ttcatgaagg
     2161 caccggagcc gaccttgacg tcgagcacga gcgccccggt cccctcggcg atcttcttgc
     2221 tcatgatcga gctggcgatg agcgggatcg cctcgacggt gcccgtgaca tcgcggaggg
     2281 cataaagctt cttgtcggcc ggcgcgaggc ccgcgcccgc cgcacagatg accgcgccga
     2341 cctcctcgag ttggcgcagc atctgctcgt tgctcaccga ggcgcgccac cctgggatgg
     2401 actcaagctt gtcgagggtg ccaccggtgt ggccgaggcc gcggccggac agttgcggca
     2461 cggccacccc gcacgccgcg acgaggggcg ccaggggcag cgtgatcatg tcgccgaccc
     2521 cgccggtgga gtgcttgtcc gcggtgggcc gcgagagcga cgagaagtcc atccgctcgc
     2581 ctgaggcgat catcgccccc gtccaccggg cgatctcgcg gcgggtcatc ccgttgagca
     2641 ggatcgccat cgcgagcgag gacatctgct cgtcggcgac aacgccccg
//
LOCUS       DAORPW010000276         2601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_14326_length_2601_cov_450.335035, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000276 DAORPW010000000
VERSION     DAORPW010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14326_length_2601_cov_450.335035"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1426
                     /locus_tag="PLB86_13215"
     CDS             176..1426
                     /locus_tag="PLB86_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HRC66151.1"
                     /translation="MANYVARIADHELDARLRVMGAVLIEGPKACGKTATASQIANTI
                     IRLDEDPVARAQLDFDPQALFSGRPPILFDEWQVEPEIWNRVRRQVDDREEKGQFILT
                     GSARPRDNANRHSGAGRFSVMQMRPMSLFETGHSTGEISLANLLAGERQAGLGTHLSF
                     DNLIRRIITGGWPDLLDSDEDFARDWLADYLRQVIEVDVPEMGHRRNPGNLRRLLESL
                     ARGVGQPVKLSDLAKDVGGEGGPVAHETITGYLDALRRLWLTDDSAAWRPHMRSRTRL
                     RTAPVRYFVDPSIGTAALSIGSAELKRDPLALGFHFEALAIRDLRIYAQLHRGQVDSW
                     RDANGNEVDAVLTARDNTWAAFEIKLNPRDVDAGAASVLKFAANVDTDRHKRPACLGV
                     ITSTGAGGLRPDGVHVIPIGALGP"
     gene            1515..1781
                     /locus_tag="PLB86_13220"
     CDS             1515..1781
                     /locus_tag="PLB86_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66152.1"
                     /translation="MKIFAVVLVTATAFGASWVLTRWASSPVWHRVVVLVALPAALVL
                     ASFNSDPLSGTTAVIAVLVGAAVGATFRPRVSGREGGRSTGPGQ"
     gene            complement(1738..>2601)
                     /locus_tag="PLB86_13225"
     CDS             complement(1738..>2601)
                     /locus_tag="PLB86_13225"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC66153.1"
                     /translation="QPCGGDHQGDAAMSGAMGGAAGGVGEATGTVGDSGADVRVHWVT
                     AFLDRPVAAFALAADFWCAALGATLSARRGENGEFATLLPTDGDAAIKLQGVGDPCQG
                     GHLDLEVPDVVAARDRAVGLGARSVADLGSLQVLRSPAGLLFCFVPLRSAGVRPPVVE
                     TPGGRVRADQVALDVGPASAPAEVEFWAALTGWRVVASVRPEFTVLIPPPELAIRLLL
                     QRLDTEPSNGPRMHLDLACADVSAATAYHRSLGAQVVAVQEFWTVLRDPAGEVYCLTA
                     RDPSTGRLPGH"
BASE COUNT          457 a          929 c          838 g          377 t
ORIGIN      
        1 cggcatgttg acgcaggtag gggctgaggg cctgcgcgag tcggttcggc atgcctgtca
       61 aggtaaccgg ttcgggcggg tgcagctgca tgaggtcgag tgcgagctag tcaaaacgtc
      121 agatccagca accgcgaaag ctcgacttca agttaggcaa acgatagagt ttggcatggc
      181 taactacgtc gcgcgcatcg ccgaccacga gcttgacgcc cgactacgcg tgatgggcgc
      241 agtcctcatc gaggggccga aggcgtgcgg aaagaccgcc actgcatccc agatcgccaa
      301 cacgatcatc cgcctcgacg aggaccccgt agcgcgcgcc cagctcgatt tcgaccccca
      361 agccctcttc tccggaaggc caccgatcct gttcgacgaa tggcaggtcg agccggagat
      421 ctggaaccgc gtccgtcggc aggtcgacga ccgcgaggaa aagggccagt tcatcctcac
      481 cggatccgcc cgaccgcgag acaacgcgaa ccgacactcg ggggccgggc gattctcggt
      541 catgcagatg cggccgatga gtctcttcga gaccgggcac tcgaccggcg agatctccct
      601 ggcgaacctc ctggcggggg aacgccaggc cggcctgggc acgcacttga gcttcgacaa
      661 cctcatccgt cgcatcatca cgggcggctg gccggaccta ctcgactcag acgaggactt
      721 cgcacgagac tggctcgccg actacctgcg tcaagtcatc gaggtcgacg tcccagagat
      781 gggccatcgc cgtaacccag gcaacctgcg ccgccttctc gaatccctag ctcgtggcgt
      841 cggacagccc gtcaagctca gcgatctcgc caaggacgtc gggggcgagg gcggcccggt
      901 cgcccacgag acgatcaccg gctacctcga cgcactcagg cgtttgtggc tcaccgacga
      961 ctctgcagcg tggcgacccc atatgcgatc tcgaacccgc ctgcgcaccg cgccggtgcg
     1021 ctacttcgtc gacccgtcga tcggcacagc cgcactcagc atcggcagcg ccgagctcaa
     1081 gcgggaccct ctcgcgctgg gcttccattt cgaagcgctg gccatccgcg acctccgaat
     1141 ctacgcacag ttgcaccgtg ggcaggtcga ctcatggcgg gacgcaaacg gcaacgaagt
     1201 cgatgcggtc ctgaccgccc gcgacaacac gtgggcagcc ttcgaaatta agctcaaccc
     1261 acgcgatgtc gacgccgggg cagcatccgt cctcaaattc gccgcgaacg tcgacaccga
     1321 ccgacacaag cggccggcct gcctgggtgt catcacctcg acgggcgccg gtggcctccg
     1381 tccagacggt gtccacgtca tcccgatcgg cgcgctcggc ccctgaactc gctgccgaac
     1441 gtcaaacgat tggcgagatg cttaacccgt tcgccgaacg cgccacatca agcgaccact
     1501 ggaggtagca acccatgaag atctttgccg ttgtgctggt gaccgcaaca gccttcggtg
     1561 cctcgtgggt gctgacgcgc tgggcgtcca gccccgtctg gcaccgcgtt gttgtgctcg
     1621 tcgcccttcc cgcggccctc gttctggcca gcttcaacag cgaccctctg tcgggcacga
     1681 ccgccgtcat cgcggtcctc gtgggcgccg cggtcggcgc gaccttccga cctcgggtca
     1741 gtggccggga aggcggccgg tcgacgggtc ccgggcagtg aggcagtaga cctcacccgc
     1801 ggggtcacgc aggacggtcc agaactcctg caccgccacc acctgcgccc caagcgagcg
     1861 atggtatgcc gtcgcggcgc tgacgtcggc gcaggccagg tccaggtgca tccggggtcc
     1921 gttgctcggt tcggtgtcca ggcgctgcag gagcaggcgg atcgccaact cgggcggtgg
     1981 gatgagcacg gtgaactcgg ggcgaaccga ggcaaccact cgccacccgg tgagcgccgc
     2041 ccaaaactcc acctcggcgg gcgcgctcgc cgggcccacg tcgagggcga cctggtcagc
     2101 gcgcacccgt ccaccgggcg tctcgacgac cggtggacgg accccggcgc tgcgcagcgg
     2161 aacgaagcag aagagcagcc cggcagggga gcgcaacacc tggagcgacc cgagatccgc
     2221 aactgaccgg gctccgaggc cgacggcgcg gtcgcgagcg gcgacgacgt cggggacctc
     2281 cagatcgagg tgtccgccct ggcacgggtc gccgacccct tggagtttga tcgcagcgtc
     2341 gccgtcggtc ggcagcaggg tggcgaactc gccgttctcg ccccgacgcg ccgacagggt
     2401 cgccccgagg gccgcacacc agaagtcggc ggctaaggcg aaggccgcaa ccgggcggtc
     2461 caggaacgcc gtcacccagt gcacccgaac atcggcgccg ctgtcaccga ccgtgcccgt
     2521 cgcctcgccg acaccgcccg cggccccgcc catcgccccg ctcatggctg catcgccttg
     2581 atgatcgcca ccgcaaggct g
//
LOCUS       DAORPW010000277         2589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_14428_length_2589_cov_918.329913, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000277 DAORPW010000000
VERSION     DAORPW010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14428_length_2589_cov_918.329913"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLB86_13230"
     CDS             <1..581
                     /locus_tag="PLB86_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRC66154.1"
                     /translation="LKADKEFKDVKVTPLLVLAKAMCLAVRRHPEINAAWDDAAQEIV
                     VKHYVNLGIAAATPRGLIVPNIKDADLMTMHELAQALGELTATAREGRTQPAQMSGGT
                     ITITNVGVFGVDSGTPIINPGESAIMAFGAIRKQPWVITNAAGEDEIAVRQVTNLALS
                     FDHRLVDGELGSRYLATVAAILEDPARGLVWG"
     gene            653..1048
                     /locus_tag="PLB86_13235"
     CDS             653..1048
                     /locus_tag="PLB86_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HRC66155.1"
                     /translation="MTKHSEGDAARVPLSYDEELRERAQKQLKAKAAFRQGVMNYVAV
                     NAFLVVLWAVTGRGSFWPIWPILGWGIGLGFQAMALNKRDSGPSAAQVQAEVDRLRQK
                     DVSMGRGPASEAPTSPGPWDRPSAPQDTH"
     gene            complement(1144..>2589)
                     /locus_tag="PLB86_13240"
     CDS             complement(1144..>2589)
                     /locus_tag="PLB86_13240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC66156.1"
                     /translation="AGGLFSLALAIVGDIVPPRERAKYQGYFLAVFGTSSVLGPIIGG
                     FFAGADSILGITGWRWVFLVNVPVGLVALFIVARTLHVSHFARPSAIDWWGAFTLVVG
                     VVPLLLVAEQGREWGWTSPNSLTAYAIGVAGLIAFIWVETRMGNAALIPMRIFKDRTI
                     AIALGGGVVVGSGMFGGMLVIPLYLQIVHGATPMDSGFQMLPMVLGMMIASVVSGQLM
                     TKTGKVRIFPIVGVLLMVIALLMLSRISADSDLRGVMALMFLFGLGLGNTMQPLTLAV
                     QAVVDPRDMGMATSAATFFRQMGGTLGVAVFLSVLFNSVGDNIKNAFVAAAKDSSFVA
                     ALSDPAVLANPTNKAFVTALSTQDTSAFGNVLADSSVINALDPRLAHPFKVGFATSMD
                     LVFLLGAIVCAAGLVILLFMPEVHLSSRSAQQRAADDAAARAAGVPGPTTGAQHTVTH
                     DLVDAVTAEAGGHSLDELPDAGEASQTRPRS"
BASE COUNT          460 a          848 c          925 g          356 t
ORIGIN      
        1 ggctcaaggc cgacaaggag ttcaaggacg tcaaggtgac gcccctgctc gtgctggcca
       61 aggcgatgtg tctggctgtg cgccggcatc ccgagatcaa cgcggcgtgg gacgacgccg
      121 cgcaggagat cgtcgtcaag cactacgtca acctcggcat cgccgcggcg accccgcggg
      181 ggctcatcgt ccccaacatc aaggacgccg acctcatgac gatgcacgag ctcgcgcagg
      241 cgctcggcga gctcaccgcg accgcgcgtg agggccgcac ccagccggcg cagatgtccg
      301 gcgggacgat cacgatcacc aacgtcggcg tcttcggcgt cgactccggc acgccgatca
      361 tcaacccggg cgagtcggcg atcatggcgt tcggggcgat ccgcaagcag ccgtgggtca
      421 tcaccaacgc ggccggcgag gacgagatcg ctgtccggca ggtgaccaac ctcgcgctgt
      481 ccttcgacca ccgtctggtc gatggcgagt tgggatcgcg ctatctcgcg acggtcgcgg
      541 ccattctcga ggacccggcg cgtggccttg tctggggctg aatagggcac actggggcat
      601 aacgcacgtg gccgacgact cctggtcggc ggtgctgacc cgggaggacc ccatgaccaa
      661 gcactctgaa ggtgacgctg cgcgcgtgcc cttgtcctac gacgaagaac tgcgcgagcg
      721 ggcacagaag caactcaagg ccaaggcggc tttccgtcag ggcgtgatga attacgtcgc
      781 cgtcaacgcc ttcctcgtgg tgctctgggc ggtcacgggg cggggttcgt tctggccgat
      841 ctggccgatc ctcggttggg gcatcggcct tggcttccag gcaatggcgc tgaacaagcg
      901 cgacagtggc ccgagtgccg cgcaggtcca ggccgaggtc gaccggttgc gtcagaagga
      961 cgtgagcatg ggccgcggcc cggcgtccga ggcaccgacc tcgcccggtc cctgggaccg
     1021 tccgtcggcc ccgcaagaca cgcactgatc tcggcgcgcg ggtgaccgcg cgatcgtcac
     1081 gcaagcgggc ggcatacccc atggtgggta tgccgcccgc tcgcgttccg tgcctcgcaa
     1141 ccctcagctg cggggccggg tttgggaggc ctcgccggcg tccggaagct cgtcgagcga
     1201 atgcccgccg gcctccgccg tcaccgcgtc gaccaggtcg tgggtgacgg tgtgttgggc
     1261 acccgtcgtc gggccgggca cgccagccgc gcgggcggca gcgtcatcgg cggcgcgctg
     1321 ctgcgcggag cgtgagctca ggtgcacctc gggcatgaac agcaggatga ccagaccggc
     1381 cgcacagacg atggcgccga ggaggaagac gaggtccatc gatgtcgcga agccgacctt
     1441 gaaggggtgc gccagccgcg ggtcgagggc attgatgacc gacgagtcgg ccaggacgtt
     1501 gccgaaagcc gaggtgtcct gcgtggacaa cgcagtgacg aacgccttgt tcgtcgggtt
     1561 ggccagcacg gccgggtcgc tgagcgctgc gacgaaggac gagtccttcg cggcggccac
     1621 gaaggcgttc ttgatgttgt cgccgacgct gttgaagagc accgagagga agaccgccac
     1681 acccagcgtg ccgcccatct ggcggaagaa ggtcgcggcg ctcgtggcca tccccatgtc
     1741 gcgcgggtcg acgaccgcct gcaccgcgag ggtgagcggc tgcatggtgt taccgagccc
     1801 gaggccgaag aggaacatca acgccatgac gccgcggagg tccgagtcgg cgctgatccg
     1861 cgagagcatc agcaacgcga tgaccatgag cagaaccccg acgatgggga agatccgcac
     1921 cttgccggtc ttggtcatca actgaccgga cacgaccgag gcgatcatca tcccgaggac
     1981 catcggcagc atctggaagc ccgagtccat cggggtcgcc ccgtggacga tctgcaggta
     2041 gagcgggatg acgagcatgc cgccgaacat ccccgagccg acgacgacgc cgccgccgag
     2101 ggcgatggcg atcgtgcggt ccttgaagat gcgcatgggg atgagggccg cgttgcccat
     2161 ccgggtctcg acccagatga aggcgatgag tccggccacc ccgatggcgt atgccgtgag
     2221 cgagttgggt gaggtccagc cccactcgcg gccctgctcg gcgaccagca gcaacggcac
     2281 gacgccgacg acgagggtga acgcgcccca ccagtcgatg gccgacggcc gggcgaagtg
     2341 ggagacgtgc agggtccggg cgacgatgaa cagggcaacc aggccgacgg ggacgttgac
     2401 gaggaacacc caacgccacc cggtgatccc gaggatcgag tcggccccgg cgaagaaccc
     2461 gccgatgatc gggccgagca ccgacgacgt gccgaagacg gccaggaagt agccctggta
     2521 tttggcgcgc tcccgcggcg gcacgatgtc gccgacgatg gccagcgcga gcgagaagag
     2581 accgccggc
//
LOCUS       DAORPW010000278         2580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_14517_length_2580_cov_979.797624, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000278 DAORPW010000000
VERSION     DAORPW010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14517_length_2580_cov_979.797624"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PLB86_13245"
     CDS             complement(<1..579)
                     /locus_tag="PLB86_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRC66157.1"
                     /translation="MTRAKVVRRTLLWVGALVVVALVAAGGFGASAVRRAWPQTSGTI
                     ALEGLGGTVSVARDERGIPTITADNATDLFRAQGFVAASDRFFEMDLRRHIGSGRLAE
                     LVGSTGLAADQAVRTMGWRKVAEAELPTLAPSTRLYLGAYADGVNDYLTKAGEPANLA
                     IEYPLLATQLPEYRVEPWSAVDSLVWLKAMAYD"
     gene            617..2221
                     /locus_tag="PLB86_13250"
     CDS             617..2221
                     /locus_tag="PLB86_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HRC66158.1"
                     /translation="MSPRLASIAGIDVPGLAAAYGLADLTRSLLVGAAVLLLALVAVR
                     VSARSGLPTMLLYLAIGFALGEDGLGLAFDDARLTQVLGYSALVFILAEGGLTTSWPG
                     IRKSVAPAAMLSTVGVLVSVGAVAAAGHLLLDLSWPVALLVAAILSSTDAAAVFSVLR
                     SVPLPRRLAGLLEAESGFNDAPVVILVTALSLRLTDADQLPWWALLLTAVLELAAGSA
                     IGLAIGWLGGRVVRRLAGGASGLFAIGVLTVVVLAYAAAASVHASGFIAAYLAALVLG
                     NMGLPHRPAVHGFAEALGTLAQIGLFVLLGLLASPSRLPAQIVPAVVIGLVLLVFARP
                     LSVFVSLTPFRIGWRDQVFLSWAGLRGAVPVVLATVPLTVGAMGTQWIFDLVVVLVVV
                     YTLVQAPTLAWVARRLGVVESVSQTSIEVETTPLEELNADLMSVSIGPESRLHGVEIF
                     ELRLPPGAAVTLVVRGSETLVPIPSTRMRRGDQMLIVTTADVRRQVEKRLIMVSRHGR
                     LAGWVSDSTGPTGPGHGNSGTRGTVP"
     gene            2264..>2580
                     /locus_tag="PLB86_13255"
     CDS             2264..>2580
                     /locus_tag="PLB86_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRC66159.1"
                     /translation="MPTYAYACTACDHAFDAVQSFSDDPLSVCPECDGRLRKVYGSIG
                     VTFKGSGFYRTDSRAASSKSSTVPASSGGSGSGSSGSGSSGSGNGSSSSGSGSGSSGS
                     SSGS"
BASE COUNT          306 a          914 c          900 g          460 t
ORIGIN      
        1 gtcgtaggcc atcgccttga gccagacgag ggagtcgacg gccgaccacg gctcgacccg
       61 atactcgggc aattgagttg ccaacagtgg atattcgatg gcgaggttgg ccggctcgcc
      121 ggccttggtg aggtagtcgt tgactccgtc ggcataggcg ccgagataga gccgggtgct
      181 cggcgccagg gtgggaagct ccgcctcggc gaccttgcgc caccccatcg tccggaccgc
      241 ctggtcggcc gcgaggcccg tcgaccccac gagctcggcc agacggccgg agccgatgtg
      301 ccgtcgcagg tccatctcga agaaccggtc cgatgccgcc acgaacccct gggcgcggaa
      361 gagatcggtc gcgttgtcgg ccgtgatcgt cggtatgccg cgctcgtccc gggcgaccga
      421 caccgtcccg cccagccctt cgagggcgat cgtcccgctg gtctgcggcc aggcacgccg
      481 cacggccgat gcaccgaagc cgcccgcggc caccaacgcc acgaccacaa gagcaccgac
      541 ccacaacagg gtccggcgga ccaccttggc acgcgtcacc ctgcgagact aagccacacc
      601 aacggaagga ccacggatgt cgccccgcct cgcgtcgatc gcgggcatcg acgtgcctgg
      661 gttggccgcg gcatacgggc tggcggacct gacccggagc ctcctggtcg gggcggcggt
      721 cctgctgctg gccttggtcg cggtgcgcgt gtcggcgcga tcgggcctgc cgacgatgct
      781 gctctacctc gccatcgggt tcgcgctcgg ggaggacggg ctggggctgg ccttcgacga
      841 tgcgcgcctc acccaggtcc tgggctattc ggcgctcgtc ttcatcctcg ccgaaggtgg
      901 cctgacgacc tcgtggccgg ggatccgcaa gtcggtcgcc ccagccgcca tgctctcgac
      961 agtcggcgtg ctcgtgtcgg tcggggcggt cgcggcggcg gggcacctcc tgctcgacct
     1021 cagttggccc gtggccctgc ttgtggccgc gatcctgtcg tcgaccgacg ccgccgcggt
     1081 gttctccgtg ttgcgtagcg tgcccctccc ccggcggttg gccgggctgc tcgaagccga
     1141 gtcgggcttc aacgacgcgc ccgtcgtcat cctcgtcacg gcgttgtcgc tgcgccttac
     1201 cgacgccgac cagttgccgt ggtgggcgct gctgctcacc gccgtgctcg agttggccgc
     1261 cggatcagcc attgggctgg ccatcgggtg gctcggtggc cgggtcgtgc gccgcctcgc
     1321 gggaggcgcg tccggattgt tcgccatcgg ggtgctcacc gtcgtcgtcc tcgcgtatgc
     1381 cgccgcggcg agcgtgcacg cctccggctt catcgcggca tacctcgcgg ccctcgtcct
     1441 cgggaacatg gggttgccgc accggcccgc ggtgcacggc ttcgccgagg cgttggggac
     1501 gctcgcccag atcggtctct tcgtgctcct cgggctgctg gccagcccga gccggctgcc
     1561 ggcccagatc gtgccggccg ttgtcatcgg tctcgtgctg ctcgtctttg cgcggccgct
     1621 gtcggtgttc gtctcgctca cgccgttccg gatcgggtgg cgcgatcaag tgttcctgtc
     1681 gtgggcgggg ctgcgcggtg ccgtgcccgt cgtcctggca accgtcccgc tgacggtcgg
     1741 cgcgatgggg acccagtgga tcttcgacct ggtcgtcgtc ctcgtcgtcg tctataccct
     1801 cgtccaggcg ccgacgctgg cgtgggtcgc gcgccgactg ggcgtcgtcg agtcagtgag
     1861 ccagacgtcg atcgaggtcg agacgactcc gttggaggag ctcaacgccg acctcatgag
     1921 cgtctccatc gggccggagt cgcggctgca cggggtcgag atcttcgagt tgcggctgcc
     1981 ccctggggcg gcagtgacgc tcgtcgtgcg cggctcggag acgctcgtgc cgatcccgag
     2041 cacccggatg cggcgcggcg accagatgct catcgtcacc accgcagacg tgcgtcggca
     2101 ggtcgagaag cgtctgatca tggtcagccg ccacgggcga ctggccggct gggtgtccga
     2161 ctccactggc ccgactggac cgggtcacgg gaattcgggc acccggggca ccgttccgta
     2221 gactggccca ccgccacatc ccgaagatcg accgaggacc accgtgccca cgtacgccta
     2281 cgcctgcacc gcctgtgacc atgcgttcga cgctgtccag tccttcagcg acgacccgtt
     2341 gtccgtgtgc cccgagtgcg acggtcggct gcgcaaggtt tacggctcga tcggcgtgac
     2401 cttcaagggg tccggcttct accgcaccga ctcccgcgcc gcctcgtcga agtcgtcgac
     2461 ggttcccgcg tcctccgggg ggtcgggctc tggctcgtcg ggttctggct cgtcgggttc
     2521 tgggaacggt tcgtcgtcgt ccgggtctgg gtctgggtcc tcaggctcat cgtctggctc
//
LOCUS       DAORPW010000279         2561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_14695_length_2561_cov_607.450519, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000279 DAORPW010000000
VERSION     DAORPW010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14695_length_2561_cov_607.450519"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1138
                     /locus_tag="PLB86_13260"
     CDS             26..1138
                     /locus_tag="PLB86_13260"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HRC66160.1"
                     /translation="MTDFLDWLAGAAATRAERGLVRSTAVSAPGLLDLAGNDYLGLRV
                     HPRVIAGAVEAATAYGGGAGASRLVTGTLPLHGELEAALATLTGSPAGLVFSTGYAAN
                     LAVMTALADADTLVVSDAHVHASLVDGSRLSRAAVAIARHNDVAHVRELLGGRPQRRA
                     VVVVESVYSVLGDAAPLADLVEVCRAHGAILVIDEAHALGVVGDRGQGMAAELGVTDH
                     PDIVLTLTLSKSLGSQGGAVLAHPAVREHLVNTARSFIYDTGLAPAAVGAAMGALDVL
                     AEQPELAKVCRDNAAALAEACGVEQPAGAVLSRAMAGPDEALAAVDRAREGGIRIGCF
                     RPPSTPDGSSRIRLTAHADHADLGPALDVLREIVGG"
     gene            1131..1847
                     /gene="bioD"
                     /locus_tag="PLB86_13265"
     CDS             1131..1847
                     /gene="bioD"
                     /locus_tag="PLB86_13265"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HRC66161.1"
                     /translation="MAEARSLPRVLVVTGTNTGVGKTVATAALAVALRARGLRVAVAK
                     PTQTGVSADEPGDLQEVCRLAGAVGLTAWEGVRLRDPLAPASAARTEGRSIPSVADYA
                     RAVGELAGAHDVVLVEGAGGLLVELDTRGGTLADLGIALRYLGIGVGVVIVAAPGLGT
                     LNHTALTVEALRARSLPIVGVVVGSWPQAPDEPDLAMRENLADLPRVAGVPLLGVLPE
                     GAGSLAPADFQVAALEWLAV"
     gene            1848..2480
                     /locus_tag="PLB86_13270"
     CDS             1848..2480
                     /locus_tag="PLB86_13270"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRC66162.1"
                     /translation="MSAPDAPATDVAATAVPRPTRIEMVCTGNICRSPMAEIILRALI
                     ERSGVSGFGGASGFGGASGFEGASEFGGVEGAAGIVVTSSGTGGWHVGDGADPRTVAV
                     LGRHGYDGSAHVASQFDASNFADLDLVLAADRGHVRELRRLARRPEDAAKVRLLREFD
                     PAAVTAGTLEVDDPWYGGTADFERCLAEVEAACHGLIDQLSDASGRALAR"
BASE COUNT          295 a          883 c          952 g          431 t
ORIGIN      
        1 cgcccgcagt gagaggatcg cccccgtgac cgacttcctt gactggttgg ctggcgccgc
       61 agcgacccgg gccgagcgcg gcttggtccg ctcaaccgcg gtcagcgcgc cgggcctgct
      121 cgacctcgcg ggcaacgact acctcggcct gcgcgtccac ccgcgcgtca tcgcgggcgc
      181 ggtcgaggcg gccacggcat acggcggcgg cgcgggggca tcacgcctgg tgacgggcac
      241 gctccccctg catggtgagt tggaggcggc cctcgccacc ctcaccggct cgcctgcggg
      301 gctggtcttc tccacggggt atgccgcgaa cctcgccgtc atgacggcgc tcgccgacgc
      361 cgacaccctc gtcgtgtccg acgcccatgt gcacgcctcg ctcgtcgacg gctcccgact
      421 ctcgcgcgct gccgtcgcca tcgcgcgaca caacgacgtc gcccacgtgc gcgagttgct
      481 cggcggtcgg ccccagcggc gggcggtggt cgtcgtcgag tcggtctact ccgtcctcgg
      541 cgacgccgct cccctggccg acctcgtcga ggtatgccgt gcccacggcg ccatcctcgt
      601 catcgacgaa gcacatgccc tcggggtcgt tggggatcgc ggtcagggca tggccgcgga
      661 gctcggcgtc accgatcacc cggacatcgt gctcaccctc accctctcga aatcccttgg
      721 ctcgcaaggc ggtgcggtcc tggctcaccc ggcagtgcgc gagcatctgg tcaacaccgc
      781 ccggtcgttc atctatgaca cgggcctggc gccggccgcc gtcggagcgg ccatgggagc
      841 cctcgacgtg ctcgccgagc agcccgagtt ggccaaggtg tgccgcgaca acgccgccgc
      901 gctggccgag gcgtgcgggg tcgagcagcc cgccggagcg gtgctctctc gggcgatggc
      961 cggccccgac gaggcgctgg cggcggtcga tcgggcgcgg gagggcggca tacggatcgg
     1021 gtgtttccgg cccccgtcga ccccggacgg gtcctcgcgg atccggctga cggcgcacgc
     1081 cgaccacgcc gacctcggtc cggcgctgga tgtgctgcgg gagatcgtcg gtggctgagg
     1141 ctcgttcgct gccgcgcgtg ctggtcgtca ccgggacgaa cacgggggtc ggcaagaccg
     1201 tcgccacggc cgcgctggcc gtcgcgttgc gcgcacgcgg gctgcgcgtg gccgtggcta
     1261 aaccgaccca gaccggcgtc tcggcggatg agcccggcga cctgcaggag gtatgccgtc
     1321 tcgccggcgc tgtcgggttg acggcgtggg agggggtgcg gcttcgcgat cccttggcgc
     1381 cggctagcgc ggcccggacc gagggacggt cgatcccttc tgtggctgac tatgcgcggg
     1441 ctgttggcga gttggctggg gctcatgacg tcgtgctggt ggaaggtgcg ggcgggctgc
     1501 tcgtggagct cgacacgcgt ggcgggactc tggctgacct cggaattgcg ttgcgctatc
     1561 tcgggattgg cgtcggcgtt gtgattgtgg ctgcgcccgg gctggggacg ctcaaccaca
     1621 cggcgctgac cgtggaagcc ctgcgcgcgc ggtcgttgcc gattgtcggc gtggtcgtgg
     1681 gaagttggcc gcaggcgccc gacgagcccg acctggccat gcgcgagaac ctcgctgatc
     1741 tgcctcgggt ggccggcgtg ccgttgttgg gggtgttgcc cgagggggcg gggagtttgg
     1801 cgccggccga cttccaggtg gctgcccttg agtggttggc tgtgtgaatg tctgctcctg
     1861 acgcgcctgc gaccgacgtg gccgccactg ccgtgccccg ccccactcgg atcgagatgg
     1921 tctgcacggg caacatctgc cgctcgccga tggccgagat catcctgcgg gctctgattg
     1981 agcgcagtgg cgtctcgggt ttcgggggcg cctcgggttt cgggggcgcc tcaggtttcg
     2041 agggagcctc agagttcggg ggcgtcgagg gtgccgctgg gatcgtggtg acgtcgtcgg
     2101 ggaccggcgg ttggcatgtc ggtgacggcg ccgacccgcg cacggtggcc gtgctggggc
     2161 gccatgggta cgacggatct gcccacgtgg caagccaatt cgacgcttca aactttgccg
     2221 atctggacct ggtcctcgct gccgaccgcg ggcacgtccg cgagttgcgg cgcctggccc
     2281 gccgacctga agacgccgcc aaggtgcgcc tgctgcggga gttcgacccg gctgcggtca
     2341 ccgctggcac gctcgaagtg gacgatccct ggtacggcgg gaccgcagac ttcgagcgct
     2401 gcttggccga agtcgaagcc gcctgccacg gcctgatcga ccagctcagc gatgcttccg
     2461 ggagggccct ggcccgctga gggtcccgcc ctggtgcttc ctgccctgtc cgtccttccg
     2521 agtgccccac ggagtgcgat tactggggtg atcaccccct c
//
LOCUS       DAORPW010000280         2538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_14930_length_2538_cov_716.787354, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000280 DAORPW010000000
VERSION     DAORPW010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14930_length_2538_cov_716.787354"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLB86_13275"
     CDS             complement(<1..794)
                     /locus_tag="PLB86_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRC66163.1"
                     /translation="MTELTIRPEEIRDALDQFVQSYDPGAASREEVGRVSDAADGIAH
                     VEGLPSAMTNELLQFEDGTLGLALNLDVHEIGVVVLGDFAGIEEGQEVKRTGEVLSVP
                     VGDAFLGRVVNPLGQPIDGLGEIKAEARRALELQAPTVMERKSVHQPLQTGLKAVDAM
                     TPIGRGQRQLIIGDRQTGKTTVAVDTIINQKRNWESGDPEQQVRCIYVAIGQKGSTIA
                     AVRGTLEDAGALEYTTIVAAPASDAAGFKYLAPYTGSAIGQHWMYAG"
     gene            complement(885..1709)
                     /locus_tag="PLB86_13280"
     CDS             complement(885..1709)
                     /locus_tag="PLB86_13280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRC66164.1"
                     /translation="MQGSSRASAAHGQRALDALLAGGPGRAVEPAALADDLFGVCGAL
                     ESSAALRRALTDPTREASAKGELVRRLFADKVGAPAVQLVTSVVGQRWSTERDLVDTV
                     EDFAVQALASGALARGGADAVEDELFRFERIVAGTPSLRDAVTDRRATPAARAEVIQA
                     LLAGKAQPETLRLARQAVLGARGRRFDRVIAGYLAIIATRREQLSATVTSAIDLDADQ
                     SQRLAAALTRIYGKPVHLKVVLDPQIVGGIRVQIGDEVVDGTVLRKLEGARRHLGG"
     gene            complement(1709..2290)
                     /locus_tag="PLB86_13285"
     CDS             complement(1709..2290)
                     /locus_tag="PLB86_13285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRC66165.1"
                     /translation="MLTIPASGGEGEGGFWATAAPIIPHPIEFLFSLVVFAGLYWVVA
                     KKVVPRLEQIYAERTAAIEGGMARAEQAQAEAKAALDSYNAQLQDARGEANRIREDAR
                     AQGAQIVAEMRTQAQAEASRIVEAAQRQIEAERQSAVTSLRGEVGRLSTDLASRIVGE
                     SLQDEVRQKGIVERFLAELESGDIKPERVGGDV"
     gene            complement(2347..>2538)
                     /gene="atpE"
                     /locus_tag="PLB86_13290"
     CDS             complement(2347..>2538)
                     /gene="atpE"
                     /locus_tag="PLB86_13290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRC66166.1"
                     /translation="ILGYGLSAIGPGIGVGLIFAAYINGVARQPEARGMLQPIAFLGM
                     AITEALAIFGIALAFVFKV"
BASE COUNT          367 a          851 c          935 g          385 t
ORIGIN      
        1 ccggcataca tccagtgctg gccgatcgcg gacccggtgt agggcgcgag gtacttgaag
       61 ccggccgcgt cggaggcggg agccgcgacg atcgtcgtgt attccagggc gccggcgtct
      121 tcgagcgtgc cgcggacggc cgcgatcgtc gagcccttct ggccgatggc gacgtagatg
      181 cagcggacct gctgctcggg gtcgccggac tcccagttgc gcttctggtt gatgatcgtg
      241 tcgaccgcga ccgtcgtctt gccggtctgg cggtcaccaa tgatgagctg gcgctggcca
      301 cgcccgatcg gggtcatcgc gtcgacggcc ttgaggccgg tctgcagcgg ctggtgcacc
      361 gacttgcgct ccatgaccgt gggggcctgc aactccagcg cgcggcgcgc ttcggccttg
      421 atctcaccga ggccgtcgat gggctggccg agcgggttga ccacgcggcc gaggaacgcg
      481 tcaccgacgg ggaccgagag gacctcgccc gtgcgcttga cctcctggcc ctcctcgatg
      541 ccggcgaagt cgccgaggac gacgacaccg atctcgtgga cgtccaggtt gagcgcgagg
      601 cccagcgtcc cgtcctcgaa ctgcagcagc tcgttggtca tcgccgaggg cagaccctcg
      661 acgtgcgcga tgccgtcggc ggcgtcggag acccgaccga cctcttcgcg gctcgcggcg
      721 cccggatcgt aggactgcac gaactggtcc agtgcgtccc ggatctcctc cggacggatc
      781 gtcagctccg tcatctgtgg tcttctcctg taatcggaat gtcgcggcgg gtgagccggt
      841 ggttcgggtg tggtgcggta tgccgtggag cggggttggg cgggtcagcc accgaggtgg
      901 cgccgcgccc cttcaagctt gcgcagcacc gtgccgtcga cgacttcgtc gccgatctgg
      961 acccggatgc cgccgacgat ctgcgggtcg agcacgacct tgaggtggac cggcttgcca
     1021 tagatgcggg tgagggccgc agccaagcgc tggctctggt cggcgtcgag gtcgatcgcg
     1081 ctggtcaccg tcgcggacag ttgctcgcgg cgagtagcga tgatcgccag gtaaccggcg
     1141 atgacccggt cgaagcgccg gccgcgggcg cccaggacgg cctggcgggc caaccgcagc
     1201 gtttccggct gcgccttgcc cgcaagcaac gcctggatga cctcggcacg tgcggcgggc
     1261 gtggcccgcc ggtcggtgac ggcgtcacga agggacgggg tgccagcgac gatccgctcg
     1321 aaccggaaca gctcgtcctc gacggcatcg gcgccgccgc gggccagtgc tccggacgcc
     1381 aaggcctgga cggcgaagtc ctcgaccgtg tcgaccaggt cgcgctcggt ggaccaccgc
     1441 tgcccgacca cgctggtcac gagctgaaca gcgggcgctc cgaccttgtc ggcgaacagc
     1501 cgccgcacga gctcgccttt ggcgctggcc tcacgagtgg ggtccgtcag ggcccggcgc
     1561 agagcagcgc tggactcgag ggcaccgcac acgccgaaga ggtcgtcggc cagggcggcc
     1621 ggttcgacgg cccggccggg tccaccggcc agcagcgcgt ccaaggctcg ctggccgtga
     1681 gcggccgacg cgcgcgagga tccctgcatc agacgtcgcc gcctacgcgc tcgggcttga
     1741 tgtcgccgga ctcgagctcg gcgaggaagc gctcgacgat gcccttctgg cggacctcgt
     1801 cctgcagcga ctcgccgaca atccggctgg ccaggtcggt cgacaagcgg cccacctcgc
     1861 cacgcagcga ggtcaccgcg ctctggcgct cggcctcgat ctggcgttgc gccgcttcga
     1921 cgatgcgaga ggcctcggcc tgagcctggg tgcgcatctc ggcaacgatc tgcgcgcctt
     1981 gggcccgggc gtcctcacgg atccggttgg cctcgccgcg ggcgtcctgc aactgagcgt
     2041 tgtaggagtc gagcgcggcc ttggcctcgg cctgggcctg ctcggcgcgg gccatgccgc
     2101 cttcgatcgc ggcggtgcgc tcggcgtaga tctgctccag ccggggcacg accttcttgg
     2161 cgacgaccca gtagaggccc gcgaagacga cgaggctgaa gaggaactcg atcgggtggg
     2221 ggatgatcgg ggccgcggtg gcccagaacc cgccctcgcc ctcgccgccg gaagcgggga
     2281 tggtcagcat gtgcacgggt gtctcctcac ggacaagcgc acggatggac gagcggaggg
     2341 tgcgggtcag accttgaaga cgaaggcgag ggcgataccg aagatcgcga gcgcctcagt
     2401 gatggccatg cccaggaagg cgatcggctg gagcatgcca cgagcctcgg gctgacgagc
     2461 gacaccgttg atgtaggcgg cgaagatcag gccgacaccg atgccggggc cgatggcgga
     2521 gaggccgtaa ccgaggat
//
LOCUS       DAORPW010000281         2496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_15353_length_2496_cov_729.968046, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000281 DAORPW010000000
VERSION     DAORPW010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15353_length_2496_cov_729.968046"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..826)
                     /gene="panB"
                     /locus_tag="PLB86_13295"
     CDS             complement(2..826)
                     /gene="panB"
                     /locus_tag="PLB86_13295"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRC66167.1"
                     /translation="MGYLDDDRWAKAWTVPSLAAAKASGRKLAMLTAYDASFARVLDR
                     HGVDLVLVGDSLGMVVRGLSSTISVTVADMSYHTAAVATGLSRALLVADLPFLADATP
                     ERALDAAQRLVQSGAQMVKLEGAGHKLDVIRFLVEREIPVCAHLGLTPQSVLRLGGYS
                     VQGREERAAARLHEDALAVAQAGASLLVLECVPSSVAASVTASIPIPTIGIGAGPGCD
                     GQVLVLHDMLGLDTGHRAPKFVKDFLGEGGSVAGAVAAYCAAVRDGSFPDAAHAYQ"
     gene            902..1840
                     /locus_tag="PLB86_13300"
     CDS             902..1840
                     /locus_tag="PLB86_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRC66168.1"
                     /translation="MALPVCKPSWLRPILVANVVAEVLIIVTGGIVRLTGSGLGCPDW
                     PTCAQGSLTPVAAQDEGFHKFIEFGNRTLTGVLIVIAVLALWAVWTALPKRRMLHLLG
                     IAMIVGIFVEAVVGGITVWTGLNPVIVAFHFLATIVLVAIATAMLRGAQDGNEVDPGS
                     RTLLVPTITARLAWVTYAVGFLVLALGTIVTGSGPHSGDADAPARTGFDPRTMSWIHA
                     DVVMLFIGLLVAMLVAVRLARVGGSQVSGSEEELALLRRSWTSVLHVSLLQGVVGYVQ
                     FFTGLPIIVVLTHMLLAAVLAIALTYGTLNLYRNPV"
     gene            1903..2307
                     /locus_tag="PLB86_13305"
     CDS             1903..2307
                     /locus_tag="PLB86_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66169.1"
                     /translation="MLTHVSTLNRIFVALFLAGGVWAAIHGRWVLVAFIVVALAYAMW
                     AIRASLRGRTTDTGRLDIGQPTDERDRLLLDRALAAVGLAALAFECGLFLYGVGGPDP
                     ASGADSGLRFAGLCLVWIIANRVVVRRDATRA"
     gene            2300..>2496
                     /locus_tag="PLB86_13310"
     CDS             2300..>2496
                     /locus_tag="PLB86_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC66170.1"
                     /translation="MHNVLRELRAQRGLSQGALGDALGVSRQTVNAIETGKYDPSLPL
                     AIKLARYFGQSVEEVFLIDGPP"
BASE COUNT          360 a          921 c          796 g          419 t
ORIGIN      
        1 ctcactggta ggcgtgcgcg gcgtcgggga agcttccgtc acggacggcg gcgcagtatg
       61 ccgcgaccgc gccggccacc gacccaccct cgccgaggaa gtccttgacg aacttcggcg
      121 cccggtggcc ggtgtccagg ccgagcatgt cgtgcagcac gaggacctga ccgtcgcacc
      181 ctggcccggc gccgatcccg atggtcggga tcgggatcga tgcggtgacg gaggccgcca
      241 ccgacgacgg cacgcactca aggacgagca ggctggcccc ggcctgcgcc acggcaaggg
      301 catcctcgtg cagccgtgcg gccgcccgct cctcgcggcc ttgcaccgag taacccccca
      361 ggcgcaacac cgattgcggg gtgagcccga ggtgggcaca caccgggatc tcccgctcga
      421 cgaggaagcg gatgacgtcg agtttgtggc cggccccttc gagcttgacc atctgggcgc
      481 cggactgcac cagacgttgg gccgcgtcca acgctcgctc aggcgtggcg tcggcaagga
      541 agggcaggtc cgccaccagc agggcccggg acagccccgt cgccaccgcc gccgtgtgat
      601 aactcatgtc ggccaccgtg accgagatgg tcgaggacag gcctctgacc accatcccca
      661 acgagtcacc gacgagcacg agatcgacgc cgtgccggtc cagcacccgc gcaaaactcg
      721 cgtcataggc cgtcagcatt gccagtttgc ggccggaagc cttggctgca gccagcgaag
      781 ggacggtcca ggccttggcc caccggtcat catcgagata gcccacccga caaccgtagt
      841 tggtgggccc ccgccggacc cgctcacgtg cggtgccgac gggcggcata cgctgtcgcc
      901 cgtggctctt cccgtctgca aaccgtcctg gctgcgtccc atcctggtgg ctaacgtcgt
      961 cgccgaggtc ctcatcatcg tcaccggcgg catcgtccga ctgaccggca gcgggctggg
     1021 gtgccccgac tggcccacct gcgcgcaagg gtcgctcacg ccggtcgccg cgcaagacga
     1081 gggattccac aagttcatcg agttcggcaa ccgcacgctc accggcgtac tgatcgtcat
     1141 cgccgtgctg gccctgtggg ccgtgtggac cgcattgccg aagcggcgca tgctgcacct
     1201 tcttggcatc gccatgatcg tcgggatctt cgtcgaggcc gtggtcggtg gcatcaccgt
     1261 ctggaccggc ctcaacccgg tgatcgtcgc gttccacttc ctcgccacga tcgtcctggt
     1321 cgccatcgcc acggcgatgc tgcgcggggc gcaggacggc aacgaggtcg acccgggctc
     1381 gcgaacgctg ctcgtcccca ccatcaccgc gcgccttgcc tgggtgacgt atgccgtcgg
     1441 cttcctcgtg ctcgccctcg gcaccatcgt caccgggtcg ggtccacact ccggtgacgc
     1501 cgatgccccc gcccgcaccg gcttcgaccc tcggacgatg tcgtggatcc acgccgacgt
     1561 cgtcatgctc ttcatcgggt tgctcgtcgc catgctcgtc gccgtccgcc tggcccgggt
     1621 cggggggtcg caggtgagcg gctccgagga ggaactggcc ctcctgcggc gctcgtggac
     1681 ctcggtcctg cacgtcagcc tgttgcaggg cgtcgtcggc tacgtgcagt tcttcacggg
     1741 gctgccgatc atcgtcgtgc tgacgcacat gctgctcgcc gcggttctgg cgatcgccct
     1801 cacctacggc acgctcaacc tctaccgcaa ccccgtctag gccacgacac gcgcagtcaa
     1861 gcacccttta cagtcaagga cacttgacat agagtcagag ccatgttgac tcacgtttcc
     1921 accctcaacc gcatcttcgt cgccctcttc ctcgctggtg gcgtctgggc cgcgatccat
     1981 gggcggtggg tgctggtcgc cttcatcgtc gtcgccctcg cctatgcgat gtgggccatc
     2041 cgggcctccc tgcgcggacg cacgaccgac accggacgcc tggacatcgg ccaacccacc
     2101 gacgagcgcg accggctgct gctcgaccgc gcgctcgccg ccgtcggcct cgctgccctt
     2161 gccttcgagt gcggcctgtt cctctacggg gtcggtgggc ccgacccagc gtccggcgcc
     2221 gattcgggcc ttcgcttcgc cggcctgtgc ctggtctgga tcatcgccaa ccgggtcgtc
     2281 gtccgacggg atgccactcg tgcataacgt gcttcgggag cttcgggcgc aacgggggct
     2341 gagccaaggc gccctcggcg atgccctcgg ggtgtcgcgc cagaccgtca acgccatcga
     2401 gaccggcaag tacgacccct cgctgccgct ggcgatcaaa ctggctcgct acttcgggca
     2461 gtccgtcgaa gaggtcttcc tcatcgacgg accccc
//
LOCUS       DAORPW010000282         2487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_15445_length_2487_cov_529.435444, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000282 DAORPW010000000
VERSION     DAORPW010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15445_length_2487_cov_529.435444"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..2074
                     /locus_tag="PLB86_13315"
     CDS             398..2074
                     /locus_tag="PLB86_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRC66171.1"
                     /translation="MSTSTHLRVDGLSFSYAGSPTSRPLLTDVTFTVSAGERVGLIGE
                     NGSGKSTLLRCLAGLLAPTGGAVTATTRGGSATLGLLHQLPPFDPDDTIADAVESAVA
                     PVRKAERSLSLAADALAAAPAAPDARSAAAAYAQALAVAESLGVWDVDARIATTLAGL
                     GLARLDRRRPTGTLSGGQQTRLSLAWLLLHRPDVLLLDEPTNHLDDAAVAFLLETLRT
                     WAGPVLFASHDRSFLDEAATSLIDLDPTAGRGPAAFTGAYTAYLSQRRESRERWERRF
                     RDEQEELRRLRQAVRDSHVVGHPGAAPRSEGRGAKKFYADRNARVVSRRVSDARSRLA
                     ELERTQVRKPPAPLSFAGLTTGYAGSASERSGPVLVASGVEVAGRLAPTSLTVDAGER
                     WLITGANGSGKSTFLAVLSGALRVTGGTVRSPSGLRVGLLGQEVSLPDPARRGRGRSA
                     AVAYADLVGGETAERLPLSSWGLLPPLVENRPVVELSVGQLRRLALAVLLAGSPDVLL
                     LDEPTNHLSLPLVDAIEEALPDYPGAVVLASHDRWLRSRWAGRRLELGSA"
     gene            complement(2091..>2487)
                     /locus_tag="PLB86_13320"
     CDS             complement(2091..>2487)
                     /locus_tag="PLB86_13320"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRC66172.1"
                     /translation="AVVRTHRRALTQADYLVVAHPNWWGKPPAIMAGWLDRVLVPGVA
                     YRLDAREGLPTSLLRLRSVLVLNTGDTPPERERQVFGDPLDDIWRRCVGAYLGGAAVH
                     RVVASPLGGSTEAEREAWLDEVGRVADAL"
BASE COUNT          332 a          880 c          891 g          384 t
ORIGIN      
        1 cgacgggagc tcggccgccg cccggatgga tggggcaccc gtgcaggatc agcgggttcg
       61 tggggagtgt catgacgacc tcacgtcgaa ggcaatcggt cgggtgaccc cacgatatgc
      121 gccggtggtg gctgccccga attcatcaca ctcgccggtg atatcgatgg tcgcaggccc
      181 ttgggggccc gatttcctga cagtcgcaag ggttctcggt gtccatgggg cgcgcgagct
      241 caacaggtga tcaggtcagc cgcgcggcaa cggaaaactc gctggcgacc ctctgcccga
      301 tccgggtagc gtcggcctcg tgctcagttg agtcgtgatc cgccccgcgc tgcggccgtc
      361 gtgcccgccg ggatgtcccg acctccgacg aggacccatg agcacctcga cccaccttcg
      421 cgtggacggc ctgtccttct cgtatgccgg ttcgcccacc tcccgcccgc tgctcaccga
      481 tgtcacgttc acggtctcgg ccggcgagcg ggtcggtctc atcggcgaga acggctcggg
      541 caagtcgacg ctattgcgtt gtctggcagg cctgctggca ccgaccggag gcgcggtcac
      601 ggcgacgacg cgcgggggca gcgccaccct cgggcttctg caccaactgc cgcccttcga
      661 ccccgacgac accatcgctg atgccgtgga gtcggcagtg gccccggtgc gcaaggccga
      721 gagatcgctc agcctggcag ccgacgccct cgcggcggcg cccgccgcgc ctgacgcacg
      781 gtcggccgcg gcggcatacg cccaggcgtt ggccgttgcc gagagcctcg gcgtgtggga
      841 cgtcgacgcg cggatcgcga cgacgctcgc gggcctcggg ctggcccgac tcgaccgacg
      901 tcgccccacc ggcacgctct cggggggtca gcagacccgg ctgtcactcg cgtggctgct
      961 gctccatcgc cctgacgtgc tgctgctcga cgagcccacc aatcacttgg acgacgccgc
     1021 cgtcgcgttc ctgctcgaga ccctgcggac ctgggcgggc ccggtgctct tcgccagcca
     1081 cgaccgatcc ttcctcgacg aggcagcgac cagcctcatc gaccttgacc cgaccgctgg
     1141 gcgcgggccg gcggcgttca ccggcgcgta cacggcatac ctcagccagc gtcgcgagag
     1201 ccgggagcgc tgggagcgac gattccgcga cgagcaggag gagctccgtc ggctgcggca
     1261 ggcggtgcgc gactcgcacg tcgtcgggca cccgggcgcc gcgccacgca gcgagggccg
     1321 cggcgcgaag aagttctacg ccgaccggaa cgcgcgcgtc gtctcgcgcc gggtcagtga
     1381 cgcgcggtcc cggctcgccg agttggagcg gacccaggtg cgcaagccgc ccgcgccact
     1441 gagcttcgcc ggcctgacca ctgggtatgc cgggtccgcc agtgagcggt cgggtccggt
     1501 cctcgtggcg tccggggtgg aggtggctgg gcggctcgcg ccgacgtcgc tgaccgtcga
     1561 tgcgggggag cggtggctca tcacgggtgc gaacgggtcg gggaagtcga catttcttgc
     1621 cgtgttgtcc ggcgcgctca gggtgaccgg ggggaccgtg cggtcgccca gtgggctgcg
     1681 ggtcggcctg cttggtcaag aggtgagcct gcctgacccg gctcgtcggg gacggggccg
     1741 ctccgcggcg gtggcctatg cggatctcgt cggtggggag acggccgagc gcctcccgct
     1801 gtcgtcgtgg gggttgctgc cgccccttgt ggagaaccgg ccagtcgtgg aactcagcgt
     1861 cgggcagctg cggcggctgg cgctggcggt gctgctcgcg ggttccccgg acgtgttgct
     1921 gctcgacgag ccgacgaacc atctgtcgct gccgctggtc gacgccatcg aggaggcgct
     1981 gcctgattat ccgggggccg tcgtcctggc cagccatgat cgttggctgc gctcacgttg
     2041 ggccggacgg cggctcgagt tgggctcggc ttgacttgcc cgcccgagat tcacaaggcg
     2101 tcagccaccc ggccgacctc gtcaagccag gcctcacgct ccgcctcggt cgatccgccg
     2161 agaggactcg ccacgacccg atggacggcc gcgcccccga ggtaggcgcc gacgcagcgc
     2221 cgccagatgt cgtccaaggg atccccgaac acctgcctct cccgctccgg tggcgtgtcc
     2281 ccggtgttga gcaccaagac agagcgcagc ctcagcagcg aggtcggcag tccctcgcgg
     2341 gcatctagtc ggtaggccac cccggggacc agcacccggt cgagccagcc ggccatgatc
     2401 gctggcggtt tgccccacca gttcgggtga gcgacaacga gatagtcggc ctgggtcaac
     2461 gcccgacgat gcgttctcac cacagca
//
LOCUS       DAORPW010000283         2482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_15500_length_2482_cov_721.079110, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000283 DAORPW010000000
VERSION     DAORPW010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15500_length_2482_cov_721.079110"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..861
                     /gene="nucS"
                     /locus_tag="PLB86_13325"
     CDS             166..861
                     /gene="nucS"
                     /locus_tag="PLB86_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HRC66173.1"
                     /translation="MRLVIARCAVDYEGRLQAHLPIATRLLLVKADGSVLVHSDGGSY
                     KPLNWMAPPCSMAESEPDEGEREAGVVAVWTVRHTKSGDTLYVRIHEVLHDSSHELGA
                     DPGLIKEGVESHLQALLAEHIHTLGAGWSLVRREHMTAIGPVDILARDANRQHVAIEI
                     KRRGEIDGVEQLTRYLELLNRDPLLAPVAGIFAAQEIKPQARTLAQDRGIRCVVLDYE
                     ALRGIDDTESRLF"
     gene            complement(866..1204)
                     /locus_tag="PLB86_13330"
     CDS             complement(866..1204)
                     /locus_tag="PLB86_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66174.1"
                     /translation="MSDLQCPARVFLARDDLPGARVEAAVEGEHIAGRYAVPPGGPAD
                     CTWLDGIADRHRGEAVIVVAPVEVITSWLLGAGRHVKPADVLSFEIDSDGWRARAVVT
                     GPIPIQRPSH"
     gene            complement(1304..>2482)
                     /locus_tag="PLB86_13335"
     CDS             complement(1304..>2482)
                     /locus_tag="PLB86_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRC66175.1"
                     /translation="AVTLSKDARARAVQLPAWNEALGLPRPWDQQWSLRIQQVLAFES
                     DLLEYDDLFNGSVVVAAKVRELVDGARAEIARVQAMGGAVAAVESGYMKSALVASHSL
                     RRQRIEAGEDIVVGLNQFTSTEPNPLTADLDTAIQTVDPAVEQAAVAAIRAWRHTRDS
                     DPAAKARADAALARLKADAASGVNLMPATLECARAKLTTGEWAEALRGEFGEYRAPTG
                     VSGSVGVSSSATPGGDLARVRADVARTGEELGEKLRVLVGKPGLDGHSNGAEQIAVRA
                     RDAGFEVIYQGIRLTPEQIVAAAVAEDVHLVGLSILSGSHLELVPDVLVGLREAGAGE
                     VPVVVGGIIPESDAATLRAQGVAAVFTPKDFGLNDIMGRFVEIIRESRGLAPLVSEPA
                     "
BASE COUNT          404 a          867 c          845 g          366 t
ORIGIN      
        1 tggtcaccat gaagaatccg ctcctttgcg ttgccctcac ccgccgttca cctagcgcgt
       61 cagtatgccg ccaagcccag tggccgaccc cgggacgtta gtcatagggt gaccgagttt
      121 ggctgcgact ggtagccgaa cggttccccg ctaggctcac cggtcgtgcg tcttgtcatc
      181 gcccgatgcg ccgtcgacta tgagggacgg ctgcaggccc acctgcccat tgccacccgg
      241 ctgctgctgg tcaaggccga cggttcggtg ctcgtgcaca gtgacggcgg ctcctacaaa
      301 ccgctcaact ggatggcccc gccctgctcg atggccgagt cggagcccga cgagggcgag
      361 cgagaggcgg gggtcgtcgc ggtctggacc gtgcggcata ccaagtctgg cgacaccctc
      421 tatgtccgga tccacgaggt cctgcacgac tcctcgcacg agctcggggc cgaccccggg
      481 ctgatcaagg agggtgtgga gtcgcacctg caggccttgc tcgccgagca catccacacg
      541 ctgggggcgg gatggtcgct ggtgcgccgc gagcacatga ccgcgatcgg tccggtcgac
      601 atcctggccc gcgacgccaa tcggcagcat gtggcgatcg agatcaagcg ccgtggcgag
      661 atcgacggcg tcgagcaact cacccgctat ctcgaactgc tcaatagaga tccgctgttg
      721 gcgccggtcg cagggatctt cgccgcacag gagatcaagc cacaggcgcg cacgctggca
      781 caggaccgtg gcatccggtg cgtcgtgctc gactatgaag cgctgcgcgg catcgacgac
      841 accgagtcac gcctgttctg aacggtcagt ggctcggacg ctggataggg atcggtccgg
      901 tgacgacggc ccgagcccgc cacccgtcag agtcgatttc gaaggacagc acgtccgccg
      961 gcttgacgtg tcgaccagca ccgagcagcc aggaggtgat gacctcgacg ggcgccacca
     1021 cgatcaccgc ctcgcctcgg tggcggtcgg cgatgccgtc cagccaggtg cagtctgccg
     1081 gtccgccggg agggacggca taccgaccgg cgatatgttc gccttcgacg gctgcctcga
     1141 cgcgcgcgcc aggcagatcg tcgcgagcga ggaacacgcg tgcaggacac tgcagatcgc
     1201 tcataccatc cagtatgaac acctcaggcc cgagcgcgag gaggagagcg gttgcattct
     1261 gcctgacgcg actcgcccag cagccctcgc gaagggcctg gacctaggcc ggctcggaaa
     1321 ccagcggagc cagcccacgc gactcgcgga tgatctcgac gaaccgaccc atgatgtcgt
     1381 tgagcccgaa gtccttgggg gtgaagacgg ccgcgactcc ctgggcccgc agggtggccg
     1441 cgtccgactc ggggatgatc cccccgacga cgacgggcac ctcgccggca ccggcctcgc
     1501 gcagcccgac gagcacgtcg gggacaagct ccaggtgcga cccggaaagg atcgacaggc
     1561 ccacgaggtg cacgtcctcg gcgaccgcgg ccgcgacgat ctgctcgggc gtcagtcgga
     1621 tgccctgata gatcacctcg aagccggcgt ctcgggcccg caccgcgatc tgctcggcac
     1681 cgttgctgtg accgtccagg ccgggcttgc cgacgagcac ccggagcttc tcgccaagct
     1741 cctcacccgt acgggcgaca tcggcccgga cccgggccag gtcgccgcct ggcgtggcgc
     1801 tggaggagac cccgaccgat ccggaaacgc cggtgggcgc gcggtattcg ccgaactccc
     1861 cgcgcaacgc ctcggcccat tcgccggtcg tcagcttcgc ccgggcgcac tccagagtgg
     1921 cgggcatgag gttgaccccg ctggccgcgt cggccttgag cctggccaga gccgcgtcag
     1981 cgcgcgcctt cgcggcgggg tcgctgtcgc gggtatgccg ccacgcccgg atcgcggcga
     2041 cagccgcctg ctcgacggcc gggtcgacgg tctggatggc ggtgtcgagg tctgcagtca
     2101 gcgggttggg ctcggtcgag gtgaactggt tgagcccgac gacgatgtcc tccccggcct
     2161 cgatccgctg gcggcgcagg gagtgcgacg cgaccagggc ggacttcata tagccggact
     2221 ccacggcagc gacggccccg cccatggcct ggacccgggc aatctcggcc cgggcaccgt
     2281 cgacgagctc acgaaccttg gcggcgacga cgaccgaccc gttgaacagg tcgtcgtatt
     2341 ccagcaggtc gctctcgaag gccaacacct gctggatccg cagcgaccac tgctggtccc
     2401 acggacgcgg aaggcccagg gcctcattcc aggccggcaa ctggacggcg cgggcgcggg
     2461 cgtccttgga cagcgtcacg gc
//
LOCUS       DAORPW010000284         2476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_15566_length_2476_cov_900.133829, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000284 DAORPW010000000
VERSION     DAORPW010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15566_length_2476_cov_900.133829"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="PLB86_13340"
     CDS             <1..1542
                     /locus_tag="PLB86_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRC66176.1"
                     /translation="WARWTWRNLTSMRTALFLLLLLSVAAVPGSIFPQRTIDPGRVGD
                     YLVDNPTTGPWLDRLGFFNVYTSPWFSAIYLLLLVSLIGCIIPRSRLHWHAIRSRPPR
                     APARLDRLAAHTTFTYAVPPAEALAAASTALRRRRYRVHSHDESSVSAETGYLRETGN
                     LVFHLALCLVIVGVAVGHLWGWRGDVIVPEGQTFTSTFRSYHTFSGGALVNPLTVPPF
                     SVRIDRFDVRFEEEVRGSQFGAPRGFTAYTTTSEGPGTATSEQVLTVNEPVSLGGADV
                     FLLGNGYAPVVTVRDAAGTVLYRQATPFLAQDNFYTSVGAIKVSAASPKMLGFSGFFM
                     PTVDPTFANGPVSEFPALKNPELALSVWEGNLFPGGRSQSVYTLNTSELTAVPKADGQ
                     PLLIRLAPGQTYTLPGDRGSISFDSVSRFAGLSVRSDPGKGLTLVAALLTIAGLVTSL
                     LVRRRRVFVRAAADPADPRTTLVTIGALSKTADPGLAAALQGLADEMAGRSSGAAPAA
                     RPERT"
     gene            1545..>2476
                     /gene="ccsA"
                     /locus_tag="PLB86_13345"
     CDS             1545..>2476
                     /gene="ccsA"
                     /locus_tag="PLB86_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRC66177.1"
                     /translation="MPFSTALPAVAIVGAAPSPELASAANLALYSAMAVFTIAMLLYT
                     AYLAALGPVSRAGQAGERAKELVAVGADATGASSGSSGSGGVVGGADGAAAAVDADDA
                     DDAAVAGPSLRARKLAGSAYGMTCLATLVLVGSVVLRGLAVMRPPWGNMFEFATAGAT
                     AAAVAYCLLARRNRWEWLGLFVVGPVLLTLGTALLAFYTEAAELMPALKSVWLIIHVT
                     VAILAVGVFVLGFSVGVVYLVKSRREATTPDRRTFWETLPSAASLERTAYGLNMVGFI
                     LWTFTLVAGAIWAQKAWGSYWTWDPKEVWTFVVWV"
BASE COUNT          290 a          903 c          850 g          433 t
ORIGIN      
        1 tgggcgcggt ggacgtggcg caacctcacc tcgatgcgca cggcgctctt cctgttgttg
       61 ctgctgtccg tcgccgccgt gcccggctcg atcttcccgc agcgcaccat cgacccgggg
      121 cgggtcgggg actacctcgt cgacaacccg accaccggcc cctggctcga ccggctgggg
      181 ttcttcaacg tctacacctc gccgtggttc tcggccatct acctgctgtt actcgtgtcg
      241 ctcatcggct gcatcatccc ccggagccgg ctgcactggc acgcgatccg ctcgcggcca
      301 ccccgcgccc ccgctcgcct ggatcgcctg gcggcccaca cgaccttcac gtatgccgtc
      361 ccgcccgccg aggcgctcgc ggcggcctcg accgcgttgc ggcgccgccg ctaccgcgtc
      421 cactcgcacg acgagtcgtc ggtcagcgcc gagaccggat atctgcgcga gaccggcaac
      481 ctcgtcttcc acctggccct ctgtttggtc atcgtcgggg tcgcggttgg acacctgtgg
      541 ggctggcgcg gcgacgtcat cgtccctgag ggccagacct tcacctcgac gttccgcagc
      601 taccacacct tctccggtgg ggccctggtc aaccccctca ccgtgccgcc gttctcggtg
      661 cggatcgacc ggttcgacgt gcgcttcgag gaggaggtcc gcggctccca gttcggcgcg
      721 ccgcgcggct tcacggcata cacgacgacc tcggagggcc ccggaacggc gacgagcgag
      781 caggtgctca ccgtcaacga acccgtgtcg ctcgggggcg ccgacgtgtt cctcctcggc
      841 aacgggtatg cccccgtcgt cacggtgcgc gacgcggcgg gcacggtcct ctaccgccag
      901 gcgacgccgt tcctggcgca ggacaacttc tacacctcgg tcggggcgat caaggtgtcg
      961 gcggcctccc cgaagatgct cggcttctcg ggattcttca tgccgaccgt cgacccgacc
     1021 ttcgccaacg ggccggtgtc ggagttcccc gcgctcaaga accccgagct cgccctgtcg
     1081 gtgtgggagg gcaacctctt cccgggcggg cgctcgcagt cggtctacac gctcaacacc
     1141 agcgagctga ccgcggtgcc caaggccgat gggcagccgc tgctcatccg gctggctccc
     1201 ggccagacct acacgctccc cggtgatcgc gggtcgatca gcttcgactc ggtgagccgg
     1261 ttcgccggcc tgtcggtgcg ctcggacccc ggcaaggggt tgaccctggt cgccgcgctg
     1321 ctgacgatcg ccggcctggt cacgtcgctg ctcgtgcggc ggcggcgggt gttcgtgcgc
     1381 gcggcggcgg accccgccga cccaaggact accctcgtca ccatcggcgc tctgtccaag
     1441 accgccgatc cgggtctcgc cgcagccctg caagggctgg ccgacgagat ggccggtcgt
     1501 tcctccggtg ccgcccccgc ggcccgtccc gaaaggacgt gagtatgccg ttctccacgg
     1561 ccctgcccgc cgtcgcgatc gtcggtgcgg caccgtcgcc ggagttggcc agcgccgcca
     1621 acctcgcgct ctactcggcg atggccgtgt tcaccatcgc gatgctgctc tacacggcat
     1681 acctcgcggc gttggggccg gtgtcccggg cggggcaggc cggcgagcgg gccaaggagc
     1741 tcgttgccgt cggtgccgac gcgacgggcg cgtccagcgg ttccagcggt tcgggtggtg
     1801 ttgtcggtgg tgctgatggc gcggcagcgg cggtcgacgc ggacgatgcg gacgatgccg
     1861 ccgtggccgg gccgtcgctg cgggcccgga agttggccgg cagcgcctac ggcatgacct
     1921 gcctggccac cctcgtgctg gtggggtccg ttgtcctgcg cgggctggcc gtcatgcggc
     1981 cgccgtgggg caacatgttt gagtttgcga ccgccggggc gaccgcggcc gccgtcgcct
     2041 actgtctgct ggcccgacgc aaccggtggg agtggctcgg cctcttcgtc gtcgggccgg
     2101 tgctgttgac cctcggcacg gcgctgctgg ccttctacac cgaggcggcc gagctgatgc
     2161 cggccctcaa gagcgtctgg ctgatcatcc acgtcaccgt cgccatcctc gcggtcgggg
     2221 tgttcgtcct cgggttttcg gtgggtgtcg tctatctggt caagtcccgc cgggaagcga
     2281 cgaccccgga ccggcgcacc ttctgggaga cgctgccgtc ggccgcgtca ctggagcgca
     2341 cggcatacgg gctcaacatg gtgggcttca tcctgtggac cttcacgctc gtggccggcg
     2401 cgatctgggc gcagaaggcg tggggctcct actggacgtg ggaccccaag gaggtctgga
     2461 cgttcgtcgt gtgggt
//
LOCUS       DAORPW010000285         2470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_15639_length_2470_cov_628.802070, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000285 DAORPW010000000
VERSION     DAORPW010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15639_length_2470_cov_628.802070"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..802)
                     /locus_tag="PLB86_13350"
     CDS             complement(14..802)
                     /locus_tag="PLB86_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRC66178.1"
                     /translation="MYISDFARLGQVSVRMLRHYDELGLLRPDHVDPWTGYRSYSPEQ
                     LHVLNRLVALKDLGFTLDQVRRILLEQVGPEELRGMLRLRRIELEDEARAVGTRLTAV
                     ESRLRMIEMEHHMSADYVIKSLPAARLVARTATLDPERLGEHIGPMFAAVAEGLAHVP
                     GALATPIATYSITEAGMDVAVGYGYAGDAPFGAEVIDLPAVEAVCGVHLGSMATIGES
                     WQAMHRWIVDNGHEFAGRCRELYLRAEGEDQSDWVTELQQPIRR"
     gene            complement(911..1210)
                     /locus_tag="PLB86_13355"
     CDS             complement(911..1210)
                     /locus_tag="PLB86_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66179.1"
                     /translation="MGWLEDLQARGQQQLAGMMAQVYDSLPPGAPRAFVDHVQLQGRP
                     AEVMIAWYVGVIGLRPEDVYGILPTQSNDITVAIKIVYLDKPEYAAARARLTGGH"
     gene            1400..>2470
                     /locus_tag="PLB86_13360"
     CDS             1400..>2470
                     /locus_tag="PLB86_13360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRC66180.1"
                     /translation="MSGATQVDPDRLLSTFLDLVSIDSPTGHEEEIGRVLEARFRAFG
                     CATTKDAIGNIVATYPGTRDGTILVATHMDTAGTDRGVVAVIGDDGIIRSDGTTILGA
                     DDKSGLAGCLELLTVLRADPEIAHPTIEFVITVGEESGLVGSRQMDVGALRATHGFVL
                     DTAGVMGSVTYWSPTSVYLTITFHGRKAHAGVEPEKGVSAVQAASRAISTMTLGRIDE
                     ETVANIGIMTGGEARNIIPDLAVLSGMARSHDQAKLDTQIESMRLACEAAALAFGATV
                     DFEAEEIYRTYKIEEDAQPYREAAAAIRSLGLEVIARKSGGGTDGNYFNAKGIPCVAL
                     TTGMVDEHATTEHIAIDDLVMAA"
BASE COUNT          419 a          828 c          845 g          378 t
ORIGIN      
        1 ggtagccaag gccctatcgc cggatgggct gctgcaactc ggtcacccag tcggactggt
       61 cctcgccctc ggcccgcaga tacagctcac ggcaccgccc ggcgaactcg tgaccgttgt
      121 cgacgatcca ccggtgcatg gcctgccagg actccccgat cgtcgccatc gaccccaagt
      181 gcaccccgca cacagcctcg accgcgggca ggtcgatgac ctcggcaccg aaaggcgcgt
      241 caccggcata cccatagccc acggcaacat ccatgccggc ctcggtgatg gagtacgtgg
      301 cgatgggggt ggcgagcgct ccggggacgt gcgcgagccc ctccgccacg gcggcgaaca
      361 tcggcccgat gtgctcgccg aggcgttcgg ggtcgagggt ggcggtgcgc gcgacgaggc
      421 gggccgcggg cagggacttg atgacgtagt cggcagacat gtggtgctcc atttcgatca
      481 tgcggagcct cgactcgacc gccgtcagcc gggtgccgac ggcgcgcgcc tcatcctcga
      541 gctcgatccg gcgcagccgg agcatgccgc gcaactcctc cggaccgacc tgctcgagca
      601 ggatgcgacg cacctgatcg agggtgaagc cgaggtcctt gagagcaacg aggcgattga
      661 ggacgtgcag ctgctcggga gaataggagc ggtagccggt ccacggatcc acgtgatcgg
      721 gccgcagcag cccgagctcg tcgtagtgac gcagcatccg caccgacacc tgaccgagtc
      781 gagcgaagtc cgagatgtac atgactccgt tctccctcct cacaccgtgt gagggtcaac
      841 tcccctgcga cgctcaactt ccccaggtgc tcgccctgcg cagagggtat gccgcccgct
      901 cgcctcgcgc tcagtgcccg ccggtcagtc gcgcccgcgc ggcggcatac tcgggcttgt
      961 cgaggtagac gatcttgatc gcgacggtga tgtcgttgct ctgggtcggc aggatcccgt
     1021 agacatcctc gggtcgcagc ccgatgaccc cgacgtacca ggcgatcatc acctccgccg
     1081 gccgcccttg cagctgcacg tgatcgacga acgcccgcgg cgcccccggc ggcagggagt
     1141 cgtagacctg cgccatcatg ccggccaact gctgctgtcc cctggcctgg agatcctcga
     1201 gccaacccat ggtgcccagg atggccgccg gagcaggctg ctcgccagga aacgcacgaa
     1261 cctcgtccgc acggtgtcgg gttgaggtca cccgcggagg gtgaaacgcg cgcgggccgc
     1321 tcgggctagc gtcgccagtg gtggaggggt tttgcgtccg aacgggcgcg gagtcttctc
     1381 gaggatcgga ggccgaacga tgtcaggcgc gacccaggtg gatccggacc ggctgctgag
     1441 caccttcctc gacctcgtct ccatcgatag cccgaccggt cacgaggagg agatcggtcg
     1501 ggtgctcgag gcgaggttcc gagcgttcgg gtgcgcgaca accaaggatg cgatcggcaa
     1561 catcgtggcg acctatccgg ggactcgcga cgggaccatc ctggtcgcca cccatatgga
     1621 cacggccggc accgaccgcg gggtggtcgc ggtcatcggt gacgacggga tcatcaggtc
     1681 cgacggcacg acgatccttg gcgcggacga caagtccggg ttggccggat gtctggagtt
     1741 gctgaccgtg ctccgagccg accccgagat tgcccatccc accatcgaat tcgtcatcac
     1801 cgtgggcgag gagagcgggc tcgtcggttc gcgccagatg gacgtcggcg ccctgcgcgc
     1861 cacccacggc ttcgtcctgg acactgccgg cgtcatgggc tcggtgacct attggtcgcc
     1921 cacgtcggtc tatctgacga tcaccttcca cggccgcaag gcgcacgccg gcgtggagcc
     1981 cgagaagggc gtgagtgccg tgcaagcggc ctcgcgggcg atctcgacga tgaccctcgg
     2041 ccggatcgac gaggagacgg tcgccaacat cggcatcatg accggcggtg aggcgcggaa
     2101 catcatcccg gacctcgcgg tcctgtcggg gatggcccga agccacgacc aagcgaagct
     2161 cgacactcag atcgagtcga tgcgcctggc ctgcgaggcg gcggcgttgg ctttcggcgc
     2221 gacggtcgac ttcgaggcgg aggagatcta tcgcacctac aagatcgagg aggacgccca
     2281 gccctatcga gaggcagccg ccgcaatccg gtcgctgggg ctggaggtga tcgcccgcaa
     2341 gtccggcggt gggacggacg gaaactactt caacgccaag gggattccgt gcgtggcgct
     2401 gacgacgggg atggtcgacg agcacgccac gactgagcac atcgccatcg atgacctggt
     2461 gatggctgcc
//
LOCUS       DAORPW010000286         2458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_15765_length_2458_cov_759.551810, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000286 DAORPW010000000
VERSION     DAORPW010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15765_length_2458_cov_759.551810"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLB86_13365"
     CDS             <1..266
                     /locus_tag="PLB86_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66181.1"
                     /translation="HGPRGRCQVSVSVGPDGAHVRVDSSHSLSTTGSTGSTGAAQPAG
                     ATGMGLVGLAERAALVGGHLSWAAHGDRFTLDLAVPAPAWAGR"
     gene            263..934
                     /locus_tag="PLB86_13370"
     CDS             263..934
                     /locus_tag="PLB86_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC66182.1"
                     /translation="MTTRVLVVDDEELVRSGIVMLLQAAPDLDVVGSAPDGPSALALL
                     DAAPAELVPAVVVTDLRMPGMSGIALTEALRRRADPPRVLVLTTFREDPDVRRVLLAG
                     ADGYLLKSAAPRDLVDAVRRVAAGETWLDPAVAGHLVAALRSAPQVADQSPTALARLT
                     ERERQVLTLMAQGLSNAEVAGQLWVEPGTVKTHVSRILHKTDTRDRSQAIALAYVSGL
                     VTVRR"
     gene            complement(1042..>2458)
                     /locus_tag="PLB86_13375"
     CDS             complement(1042..>2458)
                     /locus_tag="PLB86_13375"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HRC66183.1"
                     /translation="RRRRGRRLGGWLSTRRRWRPGSDAGQVARLVHECARPDLSSVQA
                     AAVSGQVSVPTALTVVTEYARLKPRLREEAAPTVLKGLLDMGIAEGPKGVRSLRPALL
                     AAHGADGEFQADADTAERLVSLSRPSTDELGALTYQLILDPAGAAALEAAIGPLSAPV
                     PGPDGERDTRSRQLRRGQALVEVCRRATSAGDRPPSGVKTTLMLTMSYDDLAARVGAA
                     TVIGSAQGGSLIAPETVRRLACDANVIPTVLGSRGEVLDQGRSTRLATPGQLAALCLR
                     DAGCSFPGCTTPAHWCDAHHLIHWADGGASDLTNLALLCGRHHSVVHRDQLIGHVTYA
                     ADTGVVAGAVIWNLIPGSYHRRDDGPPLLGDQPRGDRLPGGQPPGSPSLGDPPTHLTR
                     PSPDHDEWDNPAPNADSWADPWNSDEQPTTGHAARPDSAAKADPWASPRDMDDPHSAE
                     RSARHQPNPEADPWHDSGPAA"
BASE COUNT          340 a          864 c          888 g          366 t
ORIGIN      
        1 aacatggccc tcgcgggcgc tgccaggtct cggtgagcgt cggcccggac ggtgcgcacg
       61 tgcgggtcga cagcagccac agtctgtcga cgaccgggtc gaccgggtcg actggggcgg
      121 cgcagcccgc tggcgcgacg ggcatgggtc tggtggggct ggccgaacga gcggcccttg
      181 tcggcggcca cctcagctgg gccgcccacg gtgaccgatt cacgctggac ctggccgttc
      241 ctgcacccgc gtgggccggg cgatgacgac ccgggtcctg gtggtggacg acgaggagct
      301 cgtccggtca ggcatcgtca tgctgcttca ggccgctcct gacctggacg tggtcgggtc
      361 cgcgcctgac gggccgagcg ccctggcgtt gctcgacgcg gctcctgccg aactcgttcc
      421 cgccgtcgtc gtcaccgacc tgcggatgcc tggcatgagc ggtatcgctc tcaccgaagc
      481 cctgcggcgg cgcgccgatc cgccccgcgt tctggtgctc acgacgttcc gggaggatcc
      541 cgacgtccgc cgcgtactgc tcgccggggc ggatggctac ctgctgaagt cggccgcgcc
      601 acgggacctc gtcgatgccg tgcgtcgcgt ggctgcaggc gagacctggc tggaccctgc
      661 cgtcgctggc cacctggtcg cggcgttgcg ttcggcgccg caggtggccg accaatcccc
      721 cacagccttg gccaggctca ccgaacgcga acgtcaggtg ctcacgctca tggcccaggg
      781 cctgtccaat gccgaggtgg ccgggcagct ctgggtcgag ccgggcacgg tcaagacgca
      841 cgtgtcccgg atcctgcaca agacggacac gagagaccgc tcgcaggcca tcgccctcgc
      901 ctacgtcagc ggtctggtca ccgtccgccg ctagccgtgg tcggtcatat ccacggccag
      961 gcgcactcat gccccacccc gtcggacacg cagggatacc ggcgcttgcg gccacctctg
     1021 gatggggagc gaaagccggt gtcacgccgc cgggccgctg tcatgccagg ggtcggcctc
     1081 ggggtttggc tgatgccggg ccgagcgctc cgccgagtgt gggtcgtcca tgtcccgagg
     1141 gctggcccag gggtctgcct tggcggcaga gtccggccgg gcagcgtgac ccgtcgtggg
     1201 ttgttcgtcc gagttccagg ggtcagccca cgaatccgca tttggggcag ggttgtccca
     1261 ctcgtcgtga tcgggtgatg gacgcgtcag gtgtgtgggc ggatcaccga gcgatgggct
     1321 acctgggggc tggccacccg ggagacggtc gccccgcggc tggtcgccga ggagtggggg
     1381 cccgtcgtcg cgtcggtggt agctgccggg gatgaggttc cagatcacag cacccgcgac
     1441 gacgccggtg tccgccgcgt aggtgacgtg gccgatgagt tggtctcggt ggacgacgga
     1501 gtggtgtcgc ccgcacaaca acgcgaggtt ggtgaggtcg gatgccccgc cgtcggccca
     1561 gtggatcagg tggtgggcgt cgcaccaatg cgccggagtc gtgcacccag ggaaactgca
     1621 ccccgcatcg cgcaagcaga gcgcagccaa ctgtccgggt gtcgcgagcc gagtcgagcg
     1681 gccttggtcg aggacctccc cgcggctacc gaggacggtg gggatgacgt tcgcgtcgca
     1741 cgccaacctg cgcacggtct ccggcgcgat cagggagccg ccctgggcgg aaccgatcac
     1801 ggtcgcggcg ccgacgcggg cggcgaggtc gtcgtaactc atggtgagca tcagcgtggt
     1861 cttcaccccc gacggtggcc ggtcgccggc gctggtggcg cggcggcata cctcgaccag
     1921 cgcctgccca cgacgcaact ggcgactgcg ggtgtcccgt tccccgtcgg ggccgggaac
     1981 cggcgccgac agcgggccga tcgccgcctc caacgccgca gccccggccg ggtccaagat
     2041 caactggtag gtgagggcgc cgagctcgtc agttgacggg cgagacaggg agacgaggcg
     2101 ctcggcggtg tcggcgtcag cttggaactc accatccgcc ccgtgcgccg ccaacaacgc
     2161 gggccgcagc gaccggacac ccttcggacc ttccgcgatc cccatgtcca acagaccctt
     2221 caggaccgtc ggggcggctt cctcccgcag gcgtggcttc aaccgggcgt attcggtgac
     2281 cacagtgaga gccgtcggca cgctcacctg gccagagaca gcggccgcct gaacactcga
     2341 caggtccggg cgagcacact cgtggacgag ccgcgcgacc tgccccgcat cactccccgg
     2401 ccgccaacgt cggcgcgtgc tcagccaccc acccagccgt cgaccccgcc tgcgacgc
//
LOCUS       DAORPW010000287         2455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_15799_length_2455_cov_650.365833, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000287 DAORPW010000000
VERSION     DAORPW010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15799_length_2455_cov_650.365833"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLB86_13380"
     CDS             complement(<1..385)
                     /locus_tag="PLB86_13380"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRC66184.1"
                     /translation="MVEFAYEDLLPIGADETPWRLLTTEGVRTVEGPGGRQFLEVDDE
                     AMRTLTETAMHDIAHYLRPAHLAQLRKILDDPEASNNDKFVALDLLKNANIAAGGVLP
                     MCQDTGTAIVMGKRGQHVLTGGTDER"
     gene            complement(449..1384)
                     /locus_tag="PLB86_13385"
     CDS             complement(449..1384)
                     /locus_tag="PLB86_13385"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HRC66185.1"
                     /translation="MAERTAVLVVGEALIDAVVKGGEITGEHVGGSPANVAFGVGALG
                     HDVSLATWIGHDEHGDRILARCADAGVTVVPGSTGAERTSVAYASIDARGQATYRFDL
                     TWDVPELGDTSEIGHVHTGSIAATIEPGGSQVVEVIRRLRAQATVSYDPNMRPSLMGS
                     PDEVRARVEDLIALSDVVKASDEDIQWLYPGAFLPDVLRLWGELGAAVAVITRGGQGA
                     VYVVRSATTAEVTTVPAKATTVIDTVGAGDSFMAGLVSGLLDAGYLGGPDARERLRAA
                     GIDDLRPCVDRATATSGVTVGHAGAYAPTRAELGI"
     gene            1500..2072
                     /locus_tag="PLB86_13390"
     CDS             1500..2072
                     /locus_tag="PLB86_13390"
                     /inference="COORDINATES: protein motif:HMM:NF025396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 domain-containing protein"
                     /protein_id="HRC66186.1"
                     /translation="MTTPSVPSSGAQPRSRYSMGSGANMARSLLVVLALVAGLIALVP
                     RVDSVRQPAVDAASVVSYAVKDSGTPFSFPAGLPSGWTATSARFATSTDSRPTWQAGW
                     TTPAGQFVGLRQTVDATPAWVATGITGGKVTGSAEVAGRTWEIRTDERNQLYAVSVSA
                     DKLTTVVSATGGMDDIRYFIERLEPAKAAS"
     gene            complement(2134..2382)
                     /locus_tag="PLB86_13395"
     CDS             complement(2134..2382)
                     /locus_tag="PLB86_13395"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRC66187.1"
                     /translation="MPTDPASKADPADLADLTYEQARDELITIVAQLESGQVPLEQSM
                     SLWHRGEALAAYCAHWLDDAQSKIADAGVVMRIRDDEA"
BASE COUNT          350 a          831 c          880 g          394 t
ORIGIN      
        1 cccgctcgtc ggtgccgccg gtgaggacgt gctggccgcg cttgcccatg acgatggcgg
       61 tgccggtgtc ctgacacatc ggcaggacac cgccggcggc gatgttggcg ttcttcagca
      121 ggtcgagcgc gacgaacttg tcgttgttgc tggcctcggg gtcatcgagg atcttgcgca
      181 actgagccag gtgagccggg cgcaggtagt gggcgatgtc gtgcatcgcc gtctccgtga
      241 gggtgcgcat cgcctcgtca tcgacctcga ggaactgccg accgccgggt ccttcgaccg
      301 tccggacacc ctcggtcgtc aacaatcgcc agggtgtctc gtccgcgccg atcggcagca
      361 gatcctcata ggcgaactcg accatcttca ctcccacgtc gttaggggca gtccgttgtg
      421 cacttcaccc ccagcatagg aggggcgctc agatgcctag ctcggcccgg gtcggtgcgt
      481 aggcgccggc gtggccgacg gtcacgccgc tggtcgcggt agcccgatcc acacaggggc
      541 gcaggtcatc gatcccggca gcgcgcagcc gctctcgggc atccggaccg ccgaggtagc
      601 cggcgtccag cagcccggac accagcccgg ccatgaagga gtcaccggca ccgaccgtgt
      661 cgatgacggt cgtcgccttg gcggggaccg tggtgacctc agccgtcgtc gccgagcgca
      721 cgacatagac ggcgccctgg ccgccgcggg tgatgacggc gaccgcggca cccaactcgc
      781 cccagaggcg cagcacgtcg ggcaggaagg cgcccgggta gagccactgg atgtcctcgt
      841 cgctggcctt gacgacgtcg gagagcgcga tgaggtcctc gactcgggcc cggacctcat
      901 cggggctgcc catgagcgag gggcgcatgt tggggtcgta ggagaccgtg gcctgagcac
      961 gcaggcgacg gatgacctca acgacctggc tgccgccggg ctcgatggtc gcggcgatgc
     1021 ttccggtgtg gacgtggccg atctcgctgg tgtcaccgag ctcggggacg tcccaggtga
     1081 ggtcgaagcg gtaggtcgcc tgtccgcgcg cgtcgatgga cgcgtaggcg acgctggtgc
     1141 gctctgctcc ggtgctgccg ggaacgacgg tgacgcccgc gtcggcgcac ctcgcgagga
     1201 tgcggtcacc gtgctcgtcg tggccgatcc aggtggctag cgagacgtcg tgaccgaggg
     1261 cgccgacgcc gaacgcgacg ttggccggac tgccgccgac atgctcgccg gtgatctcgc
     1321 cgcccttgac gacggcgtcg atgagggcct cgcccacaac gagaacggca gtgcgttcgg
     1381 ccacggtttc ctcctgggag tagctgatag cgcctcagcc tagggctgcc cgctcgcgac
     1441 tcggcactgg tccacatccc ggtttggttg cggtctgtcc ggtcggggac gatgggggag
     1501 tgaccacacc ctcggtgccc tcgtcaggcg cgcagccgcg cagccgctac agcatgggca
     1561 gcggtgccaa catggcccga tcgctgctgg tcgtgctcgc cctcgtggcg gggttgatcg
     1621 cgttggtgcc ccgtgtcgac tcggtccgcc agccggccgt cgatgccgca tcggtcgtct
     1681 cgtatgccgt gaaggacagc ggcacgccgt tctcgttccc cgccgggctc ccctcggggt
     1741 ggacggccac gagcgcccgc tttgcaacct cgaccgactc gcggccgacc tggcaggcag
     1801 gttggacgac gccggcgggg cagttcgtcg ggttgcgcca gactgtcgac gcgacgccgg
     1861 cctgggtcgc caccggcatc acgggcggca aggtcaccgg cagcgccgag gtcgcggggc
     1921 gcacctggga gatccgcacg gacgagcgca atcagctgta tgccgtgtca gtctcggccg
     1981 acaagctcac gacggtggtc agcgccacgg gcggcatgga cgacatccgc tacttcatcg
     2041 aacggctcga gccagccaaa gccgcgagct gagcgcaggc ggcgacgcac tctgactcgg
     2101 cggcgacgcg ctcttactcg gggtgacgcg cgctcacgcc tcgtcgtcgc ggatccgcat
     2161 gacgacgccc gcatcggcga tctttgactg ggcatcgtcc aaccagtggg cgcagtaggc
     2221 cgcaagcgcc tcgccgcggt gccacagcga catgctctgc tccagcggca cctggcccga
     2281 ctcgagctgg gccacgatcg tgatgagctc gtcgcgagcc tgctcgtagg tcaggtcagc
     2341 gaggtcggcc gggtcggcct tgctggccgg gtcggtgggc acagggttgc ctctcgtgct
     2401 cgggatggag agcgggaaca ccttagcccg gttgcagacg atcccgtggt cctcg
//
LOCUS       DAORPW010000288         2401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_16432_length_2401_cov_360.396846, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000288 DAORPW010000000
VERSION     DAORPW010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16432_length_2401_cov_360.396846"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PLB86_13400"
     CDS             <1..697
                     /locus_tag="PLB86_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC66188.1"
                     /translation="DDDHAGLDVHVADLVALAGEHGGRAIFVGHSMGAFVCALLAATH
                     PDVTAGVVLVDGGLSFPFPPGLDIDATLAAALGPSLARLSMTFADEDELLAFVSTNPA
                     LGSLLDGEFGEAARTYLRHDIVRGEDGLVRSSCSLDAVRADGRGMLVHPRLPGAVREA
                     VDAGVPMELLWAPRGLFDEPQGMYDEERLAVLGLPEALRVTHVPDTNHFSILLGADGI
                     AAIGDAVERQLDA"
     gene            758..1180
                     /locus_tag="PLB86_13405"
     CDS             758..1180
                     /locus_tag="PLB86_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66189.1"
                     /translation="MDSAPISSGTGTPEDPWRLKTPPLSSDYTMHRDVRDGVEVIVCT
                     VGKTVLLYDARCVADLHAMLTAQGDWVELGSADEQKPAKEGTVEAWGRSAENPVGGWY
                     GLKKGLRGRFGMYVPPLLEHLGLAEVEHNPRGNRMRAI"
     gene            complement(1197..>2401)
                     /locus_tag="PLB86_13410"
     CDS             complement(1197..>2401)
                     /locus_tag="PLB86_13410"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC66190.1"
                     /translation="ALSVAHEAVRRHTEVIERSAAAHDRFTELIGAGGGVTDLTTALV
                     GLVGGWAVLTDAAGTRRSAHGIPPGEPVEAGAVDPLADTPLARTARASGRLTVEDMRY
                     AVPVRAGHHHLGTLVGAASQLDDADWRIVERAAAVSALVLLFERDTEAARQRRVSDLV
                     TDLLAGVLPSAEASRILRKEGLDPAVPVCVVAMRATAAPVSALVLAAAVAVGRSGIVG
                     EAGRTAVALVADADPSAAAARLAARLRSFGEVTAAGVGPVAGMDGIPDAFGEARRTLD
                     AMLALDRRGESAAAADLGFAGLVVGSAPDIAAYVERVIGPVIDYDRERGAELVKTLEA
                     YFAAGGSAARASEPLHVHVNTVVQRMSRVGRLLGADWDEPERALEVQLALRLRRLLPA
                     RPRTRPSG"
BASE COUNT          342 a          804 c          912 g          343 t
ORIGIN      
        1 cgacgacgac cacgccgggc tcgacgtcca cgtcgccgac ctggtcgcgc tggcggggga
       61 gcacggcggt cgcgcgatct tcgtggggca ttcgatgggg gcgttcgtct gcgcgttgct
      121 cgccgcgacg catcccgacg tgactgctgg tgtcgtgctg gtcgacggtg ggctgtcctt
      181 ccccttcccg cccggcctgg acatcgacgc gacgttggct gcggcgctcg gcccctcgct
      241 ggccaggctg tcgatgacct tcgccgacga ggacgagttg ctcgccttcg tctccacgaa
      301 ccccgcgttg gggtcgctgc tggacggtga gttcggcgag gccgcgcgca cctacctgcg
      361 ccacgacatc gtgcgcggcg aggacgggct cgtgcgcagt tcgtgttcgc tcgacgcggt
      421 gcgcgccgac ggacgcggca tgttggtcca cccgcgattg cctggagctg tgcgtgaggc
      481 ggtggacgcg ggtgtgccga tggagttgct ttgggcgccc cgagggctct tcgacgagcc
      541 gcaaggcatg tatgacgagg agcgactggc ggtgctcggg ctgcccgagg ccctgcgggt
      601 gacccatgtg cccgacacca accacttcag catcctgctg ggtgccgatg gcattgcggc
      661 gattggcgat gccgtcgaac ggcaactcga cgcctgagcg ctctgggtgg tgcccggcgg
      721 cgatcctgac gagccggcga cacggcatac tgcggcgatg gactcagcac cgatcagtag
      781 tggcactggc acacccgagg acccgtggcg gttgaagact ccgccgttga gttccgacta
      841 cacgatgcac cgggacgtcc gcgatggagt cgaggtcatc gtctgcacgg tcggcaagac
      901 ggtgctgctc tacgacgctc gatgcgtggc cgacctgcac gcgatgttga cggcccaggg
      961 cgactgggtg gagttgggct cggctgacga gcagaagccg gccaaggagg gcacggtcga
     1021 agcgtggggt cggtctgcgg agaatccggt gggcggctgg tacgggctca agaaggggct
     1081 gcggggccgg ttcgggatgt acgtcccgcc cctgctggag catctcggcc tggcagaggt
     1141 cgagcacaac ccccgtggca atcggatgcg cgcgatctag cctgacgcgg cgagggtcag
     1201 cccgaggggc gggtgcgggg ccgggctggg agcagtcggc gcagtcgcag ggcgagttga
     1261 acctcaaggg cgcgctccgg ctcgtcccag tcggcgccaa ggagccgacc aacgcggctc
     1321 atccgctgga cgacggtgtt gacgtgcacg tgcagcggct cgctcgcccg cgccgcgctg
     1381 ccgcctgcgg cgaaataggc ctccagggtc ttgacgagct ctgccccgcg ctcgcggtcg
     1441 tagtcgatca ccgggccgat gacccgctcc acgtaggcgg cgatgtccgg agccgagccg
     1501 acgacgagcc cagcgaagcc caggtcggca gcggcggcgc tctccccgcg ccggtcgagg
     1561 gcgagcatcg catcaagggt tcggcgggcc tccccaaagg catccggtat gccgtccatc
     1621 cccgccacgg gtcccacgcc cgcagcggtc acctcaccga aggagcgcag ccgagcggcc
     1681 aggcgggccg ctgccgcgct cgggtcggcg tcggcgacga gggccacggc ggtgcggccg
     1741 gcctcgccga cgatcccgga gcggccgacg gcgaccgcgg cggcgagcac gagagctgac
     1801 accggtgccg cggtggcccg catcgcgacg acacagaccg gcacagccgg atcgagcccc
     1861 tccttgcgca gaatccgcga cgcttcggcg gacgggagca caccggcgag cagatccgtg
     1921 accagatccg agacccggcg ctgtcgggcg gcctcggtgt cgcgctcgaa gagcaggacg
     1981 agcgcggaga cggctgcggc ccgttcgacg atgcgccaat cagcgtcgtc gagctggctg
     2041 gcggcgccga cgagggtgcc gaggtggtgg tggccggccc ggacgggcac ggcatacctc
     2101 atgtcttcga cggtgagccg gccggacgcc cgagccgtgc gcgcgagcgg cgtgtcggca
     2161 aggggatcga ccgcaccggc ttcgacgggt tcgccgggag gtatgccgtg ggcgctgcgg
     2221 cgggttcccg cggcgtcggt gagcaccgcc cagcctccga ccagtccgac gagagcggtc
     2281 gtgaggtcgg tgaccccgcc gccggcgccg atgagttcgg taaaacggtc gtgggccgca
     2341 gccgaacgct cgatgacctc ggtatgccgc cggaccgcct cgtgggcgac agacaacgcg
     2401 g
//
LOCUS       DAORPW010000289         2396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_16489_length_2396_cov_832.856472, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000289 DAORPW010000000
VERSION     DAORPW010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16489_length_2396_cov_832.856472"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PLB86_13415"
     CDS             complement(<1..1172)
                     /locus_tag="PLB86_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC66191.1"
                     /translation="MSSTPQVTSLRPIAVAAYGPTVLSSIGTGAVLPVLALTARDLGA
                     SVGTAAVIVSLLGVGMLVGDLPAGALAARFGERRALLAASVAEAVGMTAAGLAPSVVT
                     LAAAVLALGLAGSVFGLARHAYLTDAVAPSLRARALSTLGGVHRVGIFVGPFVGALVL
                     SWWGSIGPYAVGAAAALAAGGLVAVTKDLAVRDAAHRADRASTVRSREGSLTVFRVLR
                     DHRRVLTTLGFGVIAVAATRAARNAIVPLWAEAIGLDATSTSIVFGLSGAADMLLFYP
                     AGWAMDRFGRVHVAVPSMLVLGLGMVLVPFASTFWALVVVATVLGIGNGIGSGILMTL
                     GADASPEVGRAQFLGGWRLMGDIGWASGPALVSAVAVLASLGTASVVMGVVAWAGAA"
     gene            complement(1233..1646)
                     /locus_tag="PLB86_13420"
     CDS             complement(1233..1646)
                     /locus_tag="PLB86_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66192.1"
                     /translation="MTMASDAVTPVTPTWPRKAAAATYAGVAGFQVALAAGAPWGVAA
                     WGGAHEGVLPMGFRVGSVVSAGVWGLVAASLGTSWPPARWRRPVLTGLAVLAPVSFAM
                     NLATPSPVERAVWAPITAAQTVLVLLAFRAERRAR"
     gene            1713..2297
                     /locus_tag="PLB86_13425"
     CDS             1713..2297
                     /locus_tag="PLB86_13425"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRC66193.1"
                     /translation="MARSSQTHLAVLGALSVEPMTGYAVRAAITQTLGHFWSESFGQI
                     YPTLTELTARGEIRPTEPGRTSGSRFELTAAGRSRLRELLAEPAAVSPPRNGLLLRLF
                     LGGQLGSAHCRALVQESADRASAMLAGYATIRTETEAEIAAARAANDDTAAASAAYRL
                     LTLSAGEYAAHAHLAWAAETLATLATLPELAETP"
BASE COUNT          413 a          952 c          784 g          247 t
ORIGIN      
        1 gccgcgccgg cccaggccac gacccccatc accaccgacg ccgtcccgag cgaggcgagc
       61 acggcaaccg ccgacaccaa cgccgggcca ctcgcccaac cgatgtcgcc catcagccgc
      121 cagccgccga ggaactgggc ccgcccgacc tccggcgacg catcagcacc gagcgtcatg
      181 aggatccccg acccgatgcc attgccgatg cccagcaccg tcgcgacgac caccagcgcc
      241 cagaacgtcg aggcgaacgg cacgaggacc atccccagcc ccaacaccag catcgacggc
      301 acggcgacat ggacccggcc gaagcggtcc atcgcccagc ccgccggata gaacaacagc
      361 atgtcggccg cccccgacag cccgaagacg atgctcgtcg acgtggcgtc cagcccgatc
      421 gcctcggccc acagcggcac gatcgcgttg cgagccgccc gggtcgcggc gacggcaatg
      481 acgccgaacc ccaaggtggt gaggacccgc cggtgatccc gcaacacccg gaacaccgtg
      541 agcgacccct cgcgcgagcg caccgtcgac gcccggtcgg cccgatgggc ggcatcacga
      601 acggccaggt ccttggtcac tgccacgagc ccaccggcgg ccagcgcggc ggctgccccc
      661 acggcatacg ggccgatgct cccccaccac gagagaacga gcgcgccgac gaacggaccc
      721 acgaagatcc ccacccgatg cactcccccg agcgtcgaca acgcccgcgc ccgcagcgac
      781 ggcgcgaccg catcggtgag gtaggcgtgg cgcgccagcc cgaagaccga ccccgcaagc
      841 ccgagggcca acacggcggc ggcgagtgtc accacggagg gcgccagacc ggccgccgtc
      901 atgccgaccg cctccgcgac gctcgcggcg agcagggcgc ggcgctcgcc gaaccgggcc
      961 gcgagcgcgc ccgcgggcag gtcgccgacg agcatcccca ccccgagcag cgacacgatg
     1021 accgcagccg tcccgacact cgcgccgaga tcgcgggcgg tgagcgccag gacaggcagg
     1081 acagcgccgg tcccgatcga cgacagcacc gtcggcccgt atgccgcgac cgcgatcggc
     1141 cgcaggctgg tcacctgggg ggtgctgctc actcgctgca ctgtatgccg ttcggccgag
     1201 gcgcggcgga gggttccgcg ctcccggagg cgtcagcgag cgcggcgctc ggcgcggaag
     1261 gcgagcagga cgaggacggt ctgcgcggcc gtgatcgggg cccagacggc ccgttcgacc
     1321 ggggacggcg tggcgaggtt catcgcgaag gacaccggcg cgagcaccgc caagccggtg
     1381 aggaccgggc gccgccaccg ggccggcggc cacgacgtgc ccagcgacgc ggcgaccaac
     1441 ccccacaccc cggccgagac cacactgccg acgcggaaac ccatcgggag gacgccctcg
     1501 tgagcgccgc cccaggccgc gacaccccaa ggcgcccccg cggcgagcgc aacttggaac
     1561 ccggcgaccc ccgcgtaggt ggccgcggcg gcctttcgtg gccaggtcgg cgttactggc
     1621 gtcaccgcat cgctcgccat cgtcatgaac ctatctgtga gagatcttgc ccagcaccca
     1681 ccttgacata tctgtcagat ataatcaaac gcatggccag gtcctcgcaa acccatctcg
     1741 ccgtgctcgg cgccctgtcg gtcgagccga tgaccgggta tgccgtgcgc gccgccatca
     1801 cccagaccct gggccacttc tggagcgaga gcttcggcca gatctacccc acgctcaccg
     1861 agctcaccgc ccgcggcgag atccgcccca ccgagcccgg tcgcacgtcg gggagccgct
     1921 tcgagctcac cgccgccggc cgaagccgac tacgggagtt gctcgccgag ccggccgcgg
     1981 tctccccgcc gcgcaacggc ttgctgctgc gccttttcct cggagggcaa ctcgggtcgg
     2041 cccactgccg ggcgctcgtt caggagtccg ccgaccgagc cagcgcgatg ctcgcgggct
     2101 acgccacgat ccgcaccgag accgaggccg agatcgccgc cgcgcgcgcg gccaacgacg
     2161 acacggcagc cgcgtcggcg gcataccggc tgctcacgct ctcagccggg gagtatgccg
     2221 cgcacgccca cctcgcctgg gccgccgaaa ccctggcgac gctcgcgacc ctccccgagc
     2281 tcgcagaaac cccttgaaaa tcgcccgcac tgcggcttac cgtctgcggt acacgaagaa
     2341 gggaggtggt caggtagtga gttcctatcg gacacgtgag gtggctgcgc gttagc
//
LOCUS       DAORPW010000290         2392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_16535_length_2392_cov_746.615747, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000290 DAORPW010000000
VERSION     DAORPW010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16535_length_2392_cov_746.615747"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..801
                     /gene="raiA"
                     /locus_tag="PLB86_13430"
     CDS             166..801
                     /gene="raiA"
                     /locus_tag="PLB86_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRC66194.1"
                     /translation="MDIVVTGRHIQVSERFRAHLDDRLAKVAQMAPKVSRVDVVVTHE
                     RTARNSEFVELTCRDRGSIVRAEAASEDKYNALDQAVEKLLERLRRVNGRRRAAVRAG
                     RPDVGLAAVAAAPAREEAQAAPVEAPPDQATGPFGAVGDSPIEVREKVHPSEPMTLAE
                     AVNRMELVGHDFYLFNDADTGRASVVYRRRGWSYGVIHLEPAVAHDTQASG"
     gene            836..1528
                     /locus_tag="PLB86_13435"
     CDS             836..1528
                     /locus_tag="PLB86_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC66195.1"
                     /translation="MTDPWPRDHPIRVLIADDHALFRRGLRTVLELDGDIIVVGEADD
                     GMAAVEKAEATVPDVVLMDVRMPRCGGIEACQAIRERVPSARIVMLTMSDEESDLFEA
                     VRAGANGYVLKDVPGDEIAAGIRAVQSGDSLISPSMASKLLAEFAQMRRRDVEVVDSH
                     LPKLTDREVEVLGLVARGLGNREIGRELFISENTVKNHVRNILEKLQLHTRMEAAMYA
                     VQQNLIHPPPAG"
     gene            1568..>2392
                     /locus_tag="PLB86_13440"
     CDS             1568..>2392
                     /locus_tag="PLB86_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRC66196.1"
                     /translation="MRELTGAQARRIALAAQGLTAPRPASGSVGTRHLGGVIDRLGVV
                     QIDSVNVVARSHYLPFFSRLGPYDREALDRMRDAAPRRLVEYWAHEAALVAPATWPLF
                     AGRMRAAESEAWGGMRQVHAEHPGLVEAVLREVTDRGPLTSRQVEAALAHDVPRSRED
                     WGWNWSAVKRALEWLFWSGQVASAGRTASFERRYAAPSRVAPPALRAAWAARDDARTE
                     GEAITELVRMAASALGVATEASLADYFRLTRAQARDAIAVLCAEGCIEEVRVVGWRD"
BASE COUNT          358 a          788 c          867 g          379 t
ORIGIN      
        1 gtcgcagcga cgcaacgccg tgggcgcgcc gacgtgccct ctggcttgac tcggtgaagc
       61 gggctacggt ggaaggacat cgtccgagac gtctgggggt gatgatcagg tttgcggggt
      121 ctgcccggcc cgccacctcg tcacgtcgca cgtcaaggag gaacagtgga catcgtcgtc
      181 accggtcgtc acatccaggt ttcagagcgt ttccgggcac acctggacga caggctggcc
      241 aaggtcgcac agatggcgcc caaggtctca cgggtcgatg tcgtcgtcac ccatgagcgg
      301 acggctcgca actcggagtt cgtcgagctc acctgccgcg atcgggggtc gatcgtgcgc
      361 gccgaagcgg ccagtgagga caagtacaac gccctcgacc aggccgtcga gaagctcctg
      421 gagcggctgc ggcgggtcaa cgggcggcgc cgggctgcgg tgcgcgcggg gcgccccgac
      481 gtgggcctgg ctgcggtggc tgcggcgccg gcgcgagagg aggcgcaggc cgcaccggtc
      541 gaggctccgc ccgaccaggc caccggcccg ttcggagctg tcggcgactc gcccattgag
      601 gtgcgcgaga aggtccaccc gagcgagccg atgaccctgg ccgaggcggt gaaccggatg
      661 gagttggtcg ggcatgactt ctacctgttc aacgacgccg acactggccg cgcgagcgtc
      721 gtctatcggc gccgtgggtg gtcctacggg gtcattcatc tggagcctgc agtcgcgcac
      781 gacactcagg catccggctg aggcgcgcgt cacatgggca tgacatcatg cccatgtgac
      841 cgatccatgg ccccgtgacc acccgattcg ggtgcttatc gccgacgacc atgcactctt
      901 caggcgcggc ctgcgcacgg tcctggaact cgacggtgac atcatcgttg tcggagaagc
      961 cgacgacggc atggctgccg tcgagaaggc cgaggccacc gtccccgacg ttgtgctcat
     1021 ggatgtccga atgcctcgct gcggcggcat cgaggcctgc caggcgatcc gcgagcgggt
     1081 gccgtcagcg cgcatcgtca tgttgacgat gtccgacgag gagtcggatc tcttcgaagc
     1141 ggtgcgcgct ggcgccaacg ggtacgtcct caaggacgtc ccaggcgatg agattgcggc
     1201 gggcatccgc gctgtgcaga gcggcgattc gctcatctcg ccgtccatgg cctccaaact
     1261 gcttgccgaa ttcgcccaga tgcgccgccg cgacgtcgag gtcgtcgaca gccacctgcc
     1321 caagctcacg gatcgcgagg tcgaggtgct cggcctggtg gcccgcggcc tgggcaaccg
     1381 cgagatcggc cgcgagttgt tcatctcgga gaacaccgtg aagaaccacg tccgcaacat
     1441 cctggagaag ctccagttgc acacccggat ggaagcggcg atgtatgccg tgcagcagaa
     1501 cctcattcac ccgccaccgg ccgggtgagc ggcggcatac ctgctggggg agtgcctcgg
     1561 gtgagcgatg cgtgagctga ctggagcgca ggcccggcgg atcgcgttgg ccgctcaagg
     1621 gttgaccgcg ccgcgtcccg catccggctc ggttggcacc cggcacctgg gcggcgtcat
     1681 tgaccggctc ggcgtcgtcc agatcgacag cgtcaacgtc gtggcgcgca gtcattacct
     1741 gccgttcttc agccgcctcg gcccctatga ccgcgaggcg ttggatcgga tgcgcgacgc
     1801 cgcgccgcgt cgtctcgtcg aatactgggc gcacgaggcg gccctcgtcg cgcccgcgac
     1861 ctggccgctg ttcgccggtc ggatgcgggc tgcggagtcc gaggcgtggg gcggcatgcg
     1921 tcaggtccac gccgaacacc cgggccttgt cgaggccgtg ctgcgggagg tcactgatcg
     1981 cggcccgctg acctcgcgcc aggtagaggc cgcgctcgcg cacgacgtac cgcgatcccg
     2041 ggaggactgg ggctggaact ggtcggcggt caagcgggcg ttggaatggc tcttctggtc
     2101 ggggcaggtc gcctcagcgg ggcgcactgc gtccttcgag cgtcggtatg ccgcccccag
     2161 ccgggtcgct ccgccggccc tccgagcagc gtgggctgct cgggacgacg cccggacgga
     2221 gggcgaggcg atcaccgagc tggttcggat ggctgccagc gccctcggtg tggccacgga
     2281 ggcgagcctg gccgactact tccgcctgac ccgggctcag gcgcgcgacg ccatcgcggt
     2341 cctgtgcgcg gagggctgca tcgaagaggt gagggtcgtt gggtggcggg ac
//
LOCUS       DAORPW010000291         2358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_16945_length_2358_cov_280.540599, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000291 DAORPW010000000
VERSION     DAORPW010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16945_length_2358_cov_280.540599"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..401
                     /locus_tag="PLB86_13445"
     CDS             153..401
                     /locus_tag="PLB86_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66197.1"
                     /translation="MTPPRSTFTSENGEALLQRHFDRVTRVDIETIATFPDHASAAAY
                     LATVDVTWAASLPHFEGARQDTGFTTVFTATAPRAATR"
     gene            complement(485..1300)
                     /locus_tag="PLB86_13450"
     CDS             complement(485..1300)
                     /locus_tag="PLB86_13450"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRC66198.1"
                     /translation="MAGAASRTENDGRVVRLAVLIDADNARAAVIEPILAEVAGVGEA
                     TVRRIYGDLTAPQSASWRGVLQRHAIKPVQQFAYSVGKNATDSALIIDAMDLLYTGRF
                     DGFCLVTSDSDFTGLAMRLRESGLLVLGFGERKTPEAFRNACHRFVFIDLPEASQAHG
                     EGDEDAMSDAPNLVAGASATKRSAASTTGLAAKPPFPKKFVLKALRESLDDSGWAPLS
                     TFGNYLIKLQPDFDARLFGYRKLSDLVRGRADLFRVEERPPAGDHRVIYIAPR"
     gene            1498..2274
                     /locus_tag="PLB86_13455"
     CDS             1498..2274
                     /locus_tag="PLB86_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66199.1"
                     /translation="MPEILPHAAVSRLLTERGWVASWGDLRTVADRGTLETLLAAGHL
                     QRVARGRYAAPVEIPARLAAERVAGVASHRTAAQHYGLAMKWVPSQPCVTVRRGRSLG
                     GRGKGLHLSYRSLPTSDIDGWITTRLRTVLDCAATLPFDEALTIADSALRDRKITRAQ
                     LVDRAATWPTRGRQRVLRVAAHADPRASGPIESVLRAICLEIPGLRVTPQVRIERSAR
                     FLGWSTSPTSGCVSSSRLRASNSTAIGRGSTAIAAATTSW"
BASE COUNT          365 a          815 c          791 g          387 t
ORIGIN      
        1 gcgctgccag gagtcgacta cctcgccacc gatgcgtcac ccgcgatggt ggccgaggca
       61 agagccaagg gtgtgcgggc ggaggtgcgg tcagccgagg ccaccggaca accgactggc
      121 tcgatcgatg tggtggtcgc ggcctggatg ctgtgacccc gcctcgctcg acgttcacct
      181 ccgagaacgg tgaggccttg ttgcagcggc acttcgaccg ggtcactcgc gtcgacatcg
      241 agaccatcgc caccttcccc gatcacgcca gcgccgcggc atacctcgcc accgtggacg
      301 tgacgtgggc ggcgtccctt ccgcacttcg agggtgcccg tcaggacacc ggattcacga
      361 cggtgttcac ggcgaccgca cctcgggcgg ccactcgctg actgacgtcc ggcaaacggg
      421 tatgccgccc gcctccccca tgcgcccgcc gttcgtgggt ccgcggggta tcacacgtcc
      481 atcgtcaccg tggcgcgatg tagatgactc ggtggtcgcc ggccgggggg cgttcctcaa
      541 cccggaacag gtccgcacgg ccacgtacca ggtcggacag tttccggtag ccgaacagcc
      601 gcgcgtcgaa gtccggctgc agtttgatga ggtaattgcc aaacgtgctc agcggtgccc
      661 accccgagtc gtcgagggac tcgcgaagcg ccttcaggac gaacttcttc gggaacggtg
      721 gcttcgccgc cagcccggtg gtggacgccg cggaccgctt cgtagcgctg gctccggcaa
      781 ccaggttcgg ggcgtcggac atggcgtctt catccccctc gccgtgggcc tgcgaggctt
      841 ccgggaggtc gatgaagacg aagcggtggc aggcgttccg gaaggcctct ggggtcttgc
      901 gttcgccgaa acccagaacg agcaaacccg attcgcgcag tcgcatggcc aagcccgtga
      961 agtcactgtc gctggtgacg aggcaaaacc cgtcgaaccg tccggtgtag agcaggtcca
     1021 tcgcatcgat gatgagcgca ctgtcggtgg cgttcttgcc gaccgagtag gcgaactgct
     1081 gaacgggttt gatggcatgc cgctgcaaca cgccgcgcca ggaagcgctc tggggtgcgg
     1141 tgaggtcacc atagatccgt cgaacggtcg cctcgcccac gccggcgacc tcggcgagga
     1201 tcggctcgat cacggcggcc cgcgcattgt ccgcgtcgat cagcaccgcg agacgaacga
     1261 cccgcccgtc attttcggtt cgcgacgccg cgccagccac gttcatgctt cggaccatat
     1321 cccggcgccg acggtgccgg gataacgagc ggtccgtgaa cagaccgaat gccattggtc
     1381 tgcggccggg acgcctgttg agcgtgttca gccccggagg gccagaccat tggtattcgg
     1441 agtgcaggct gtggataacg tcagcgccaa cgctggcgcg cgcggcactc ttgtcgggtg
     1501 cccgaaatcc tcccccacgc cgcggtgagc cgtctgctca ccgagcgcgg ttgggtggcg
     1561 tcgtggggcg accttcgaac cgtcgccgac cgcggcaccc tcgaaaccct cctcgccgcc
     1621 ggtcacctcc agcgcgtcgc gcgtggccgg tatgccgccc ccgtcgagat cccagcccgg
     1681 ctcgccgctg aacgcgtcgc cggcgttgcc tcccatcgga cggcggcgca gcactacggg
     1741 ctcgccatga agtgggtgcc aagccagcct tgcgtcaccg tccgacgcgg gcggagcctc
     1801 ggtggccgcg gcaaggggct gcacctgtcc tatcgatcgc tgccaacttc tgacatcgac
     1861 ggatggatca ccacgcggct gcgaaccgtc ctggactgcg ccgcgaccct ccccttcgac
     1921 gaggcactaa cgatcgccga ctccgcgctt cgcgaccgca agatcacccg tgcccagctc
     1981 gtcgaccgcg ccgccacctg gccgacgcgt gggcggcagc gagtgctgcg agtggccgcc
     2041 cacgccgacc ctcgggcttc cgggcccatc gaatcggtcc tgcgcgcgat ctgccttgag
     2101 atcccagggt tgagggtcac cccgcaggtg cggatcgagc ggtcggcgcg cttcttgggc
     2161 tggtcgacct cgccgacgag cggctgcgta tcgtcatcga ggctgagggc ttcgaattcc
     2221 acggcgatag ggaggggttc gaccgcgatt gccgccgcta cgacgagctg gtagccgaag
     2281 ggtgggtggt gctgcgcttc acgtgggagc acatcatgcg tcgtgatcgc tgggtcgccc
     2341 agatcctcgc agccaccg
//
LOCUS       DAORPW010000292         2335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_17236_length_2335_cov_886.558772, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000292 DAORPW010000000
VERSION     DAORPW010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17236_length_2335_cov_886.558772"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PLB86_13460"
     CDS             <1..1112
                     /locus_tag="PLB86_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRC66200.1"
                     /translation="YEQAREEVARFIGAREDDLVVFTRTTTDSWNLLARTLPADTTVV
                     VFASEHHSTLLPWGTRTSGATVTLPVPRTVEDGLAWLDSALAESTGAHTLVVVSGASN
                     VTGEVWPVEQIVAVAHRHGARVALDAAQLAPHRAVDLAASGVDYVAFSGHKLYAPYGA
                     GVLAGRADWLDAAEPYLVGGGATARVGQCGITWATGAARHEGGSPNVVGAVALAAACA
                     VLQERRDDLARYEHTLAERLRRGLEQIPGVHTHSLFGAGSDRVGVVAFTIDGLDSSLV
                     STALSVEHGIGVRDGKFCAHPLVDALLADGDEGTPTTAVRASLGLASRPEHVERLVSA
                     VTELAASGPGLTYTRGPQGWAPESDPRVVDLPRPW"
     assembly_gap    1113..1212
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1228..2127)
                     /locus_tag="PLB86_13465"
     CDS             complement(1228..2127)
                     /locus_tag="PLB86_13465"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRC66201.1"
                     /translation="MRWLDQATFLVAVVQAVVPVAGIVIVATTLLAAVSRRRRTALVG
                     AIASVVVLVGAAPFVIPSGRAEPGPDDVVVMSSNLQYGRADAVALTERVRALDVDILV
                     LLEVTPSAVDLLRAAGLEAMLPFVSGHPRAGADGTMIRSRFPLTELPTPTFPGEVNLS
                     QPVARVDLPGTSSAPAGRPMLIRAVHPMPPTDPNLGFWRSALDGLATWAHSQPDGIPL
                     VLAGDFNASADHPGYRQVADGLVDAHRAVGAGWVRTWPLGRRWLPPFVQIDHILARGL
                     TVTGAGSEVIAGTDHALVWASWR"
     gene            complement(2198..>2335)
                     /locus_tag="PLB86_13470"
     CDS             complement(2198..>2335)
                     /locus_tag="PLB86_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66202.1"
                     /translation="AIIAATEGGITDEHQLQQVIRRTVGGWAGGKIRRRPMIIPIVIE
                     A"
BASE COUNT          305 a          854 c          784 g          292 t
ORIGIN      
        1 ggtacgagca ggcccgcgag gaggtcgccc gcttcatcgg cgcccgcgag gacgacctgg
       61 tggtcttcac ccgcacgacg accgattcgt ggaacctgct ggcccgcacc cttccggccg
      121 acacgaccgt cgtcgtcttc gcctccgagc accactcgac cctgctcccc tggggcacgc
      181 gcacgtcggg cgcgaccgtg accctcccgg tgccgcgcac cgtcgaggac gggttggcct
      241 ggctcgacag cgccctcgcc gagtcgaccg gggcgcacac cctcgttgtc gtgtccggcg
      301 cctcgaacgt caccggtgag gtctggccgg tcgagcagat cgtcgccgtg gcccaccggc
      361 acggcgcccg ggtcgccctc gatgccgcgc agcttgcccc gcaccgggcg gtcgacctcg
      421 ccgcctccgg cgtcgactac gtcgcgttct ccggccacaa gctctacgcc ccctacggcg
      481 caggcgtcct cgccggtcgg gccgactggc tcgacgccgc cgagccctac ctcgtcggcg
      541 gtggcgcgac ggctcgcgtc ggccagtgcg ggatcacctg ggccaccggt gcggcgcgtc
      601 acgagggcgg cagccccaac gtcgtcggcg ccgtcgccct cgccgcggcc tgcgctgtcc
      661 tgcaggagcg ccgggacgac ctcgcgcgtt acgagcacac cctcgccgag cggctgcgcc
      721 gcggcctgga gcagatcccc ggcgtgcaca cccactcact cttcggcgcc ggctccgacc
      781 gggtcggcgt ggtcgccttc acaatcgacg gtctggactc ctccctcgtg tcgaccgctc
      841 tgtcggtcga gcacgggatc ggcgtccgcg acggcaagtt ctgcgcccac ccgctcgtcg
      901 acgcgctcct cgcggacggc gacgagggca cccccacgac ggccgtccgg gccagcctgg
      961 gcctggcctc ccgccccgag cacgtcgagc ggctcgtctc agcggtcacc gagctcgcgg
     1021 cctctggccc gggcctgacc tacacccgtg ggccgcaggg ctgggcgccc gagtccgacc
     1081 cgcgcgtcgt cgacctcccc cgcccctggt agnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nntagtccgg ccggcgctca gcgccaactc gcccagacga gggcgtgatc
     1261 agtgccggcg atgacctccg agccggcgcc ggtgaccgtt agtccccgag ccaggatgtg
     1321 gtcgatctgg acgaacggcg gcagccatcg ccgccccagc ggccaggtgc ggacccaccc
     1381 ggcgccgacg gcccggtggg catcgacgag gccgtcagca acttggcgat agcccggatg
     1441 gtcggcagag gcgttgaagt cccccgccaa caccaggggt atgccgtccg gctggctatg
     1501 cgcccacgtc gccagtccgt cgagtgctga gcgccagaac ccgaggttcg gatcggtcgg
     1561 gggcatcggg tgtacggccc tgatcaacat cgggcgcccg gcaggggccg agctcgtgcc
     1621 ggggagatcc acccgagcga ccggctggct gaggttgacc tcaccgggga aggtcggcgt
     1681 gggcagttcc gtgagtggga accggctgcg gatcatcgtc ccgtcagcgc ccgcacgggg
     1741 atgccccgag acaaagggca gcatggcctc caaccctgcg gcgcgcagca ggtcgacggc
     1801 ggacggagtc acctccagca ggacgaggat gtcgacgtcc aacgcccgaa cccgctcggt
     1861 gagggcaacc gcgtcggccc gtccgtattg cagattgctc gacatgacga cgacgtcgtc
     1921 cggccccggc tcagcccggc ccgaggggat gacgaacggc gcggcaccga cgaggacgac
     1981 caccgacgcg atggccccga ccagggcggt ccgccgccgc cgcgacacgg cggccagcag
     2041 cgtggtcgcc acgatgacga tccccgcgac agggacgacg gcctggacga cggcgacgag
     2101 gaaggtcgcc tggtcgagcc agcgcacggc atacatcgtg gcggcgccgg ccagcgccgc
     2161 ggcgagcagc ccgctgccga gccggtggcg cgccaggtca ggcctcgatg acgatcggga
     2221 tgatcatcgg acggcgccgg atcttgccgc ccgcccagcc gccgacggtg cgccggatca
     2281 cctgctggag ttggtgttcg tcggtgatgc ccccctcggt ggccgcgatg atcgc
//
LOCUS       DAORPW010000293         2330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_17301_length_2330_cov_747.220220, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000293 DAORPW010000000
VERSION     DAORPW010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17301_length_2330_cov_747.220220"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="PLB86_13475"
     CDS             complement(<1..1265)
                     /locus_tag="PLB86_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HRC66203.1"
                     /translation="MALEITSARPDPALFDLPWSTPLEDWPESHLAALPRGISRHIVR
                     FVKLSGGVLAVKEIKADIAAREFGMLRSLVRMDLPCVTPVGVVTGRIGTDGEPLDACL
                     ITRHLQFSLPYRALYSQALRPDTAARLVDALAVLLVKLHLDGFWWGDVSLSNTLFRRD
                     AGAFAAYLVDAETGEMHARLSDGQRAHDLEIARVNIAGELMDLQAGGFLEDHTDPVET
                     ATEIVRRYESLWSELTGVERFETGDRWRVDERIRRLNALGFDVGELAMSTDIGGTTIQ
                     IQPKVVDAGHHSRRLLRLTGLDVEENQARRLLNDLDSFRAATDRLGDDESVVAHDWLV
                     NVYEPTVRAVPRELRGKLEAAEVFHEVLEHRWYLSERAGRDLPLADTIQDYVRTVLPT
                     KPDESAVLGIDTRAMPVLGGDLDLDGDSDL"
     gene            complement(1426..>2330)
                     /locus_tag="PLB86_13480"
     CDS             complement(1426..>2330)
                     /locus_tag="PLB86_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC66204.1"
                     /translation="THLSPKDRDVAMVFQNYALYPHMSVADNMGFALKIAGVSKDEIA
                     KRVLEAAKILDLEPYLERKPKALSGGQRQRVAMGRAIVRSPQVFLMDEPLSNLDAKLR
                     VQTRTQIASLQRRLGVTTVYVTHDQTEAMTMGDRVAVLKDGYLQQCDAPRRMYDHPAN
                     VFVAGFIGSPAMNLLDVPVVDGGVKLGAATVAIPRETLAKASGSRVTLGVRPEDLDLS
                     SDGVGLQVTVDVVEELGADAYVYGTPAGVQQIGTDGDEVGNKPFLARVDGRRPPKRGD
                     TVYLVPKPGHIHLFDSASGVRLND"
BASE COUNT          365 a          739 c          870 g          356 t
ORIGIN      
        1 aggtcggaat cgccgtcgag atcgaggtcg ccgccgagga ccggcatggc gcgggtgtcg
       61 atgcccagca cggcactttc gtcgggcttg gtcggcagca cggtccgcac gtagtcctgg
      121 atcgtgtcgg caagggggag gtcacgcccg gcgcgctcgg acaagtacca ccggtgctcc
      181 aacacctcgt ggaacacctc cgcggcctcc aacttgccgc gcaactcgcg cggcaccgcc
      241 cgcacggtcg gctcgtagac gttgaccaac cagtcgtggg cgaccacgga ctcgtcgtca
      301 cccaggcggt cggtggccgc ccggaacgag tcgaggtcgt tgagcagccg ccgggcctgg
      361 ttctcctcga cgtccaggcc ggtgagacgc agcagccgac ggctgtggtg gccggcgtcg
      421 acgaccttcg gctggatctg gatcgtcgtg ccgccgatgt cggtcgacat cgcgagctca
      481 cccacgtcga agccgagtgc gttgagccgc cggatccgct cgtcgacgcg ccaacggtcg
      541 ccggtctcga accgctcgac cccggtgagc tcggaccaca gcgactcgta gcgacggacg
      601 atctccgtcg ccgtctcgac cgggtcggtg tggtcctcga ggaagccgcc ggcctgcagg
      661 tccatcagct caccggcgat gttgacccgc gcgatctcca ggtcgtgggc ccgctgaccg
      721 tccgacagcc gcgcgtgcat ctcgccggtc tcggcgtcca cgaggtatgc cgcgaacgcg
      781 ccggcatcgc ggcggaacaa ggtgttggag agcgagacgt cgccccacca gaacccgtcg
      841 aggtgcagtt tgacgagcag gacggccagg gcgtcgacga ggcgggcggc cgtgtcgggt
      901 cgcagcgctt gcgaatagag cgcgcgatag ggcagcgaga actgcaggtg ccgggtgatg
      961 aggcacgcgt ccaacggctc gccatcggtg ccgatccgcc cggtgacgac gccgaccgga
     1021 gtgacgcacg gcaggtccat ccgcacgagg gagcgcaaca tgccgaactc ccgggcggcg
     1081 atgtcagcct tgatctcctt gacggccagg actccgccgg acagtttgac gaagcggacg
     1141 atgtgccgcg agataccgcg ggggagggcg gcgaggtggc tttccggcca gtcctccaga
     1201 ggcgtcgacc acggcaggtc gaacagcgcc gggtcgggac gggcagaggt gatttccagt
     1261 gccacagtga gcgattcgct tccagatgag tgctgccgga cgaagcaggt cggtggtccg
     1321 ttgacgggac agcacgcggc cggcaccttc ggatcgaagg cgccggccgc ggcataccgt
     1381 cattggcgta tgccggtccg cgcaggcgtg gaccggcggc ggcgctcagt cgttgaggcg
     1441 aaccccgctg gctgagtcga acaggtggat gtggcccggc ttggggacga ggtagaccgt
     1501 gtcgccacgc ttggggggac ggcgaccgtc gacgcgggcg aggaacggct tgttgccgac
     1561 ttcgtcaccg tcggtgccga tctgctgcac acctgcgggg gtgccataga cgtaggcgtc
     1621 ggcgccgagc tcttcgacga cgtcgaccgt gacctgcagg cccaccccgt cgctcgacag
     1681 atcgaggtct tcggggcgga cacccagggt gacccggctg ccgctggcct tggccagggt
     1741 ctcgcgcggg atggcgaccg tggcggcgcc gagcttgacc ccaccgtcga cgaccgggac
     1801 atcgagcagg ttcatcgcgg gcgagccgat gaagccggcg acgaagacgt tggcggggtg
     1861 gtcgtacatc cggcgcgggg cgtcgcactg ctggaggtag ccgtccttga ggaccgcaac
     1921 gcggtcaccc atggtcatgg cctcggtctg gtcgtgggtg acgtagaccg tggtgacgcc
     1981 caggcggcgc tgcagcgacg cgatctgggt gcgggtctgc acgcggagct tggcgtcgag
     2041 gttggacagc ggctcgtcca tgaggaagac ctggggggag cggacgatgg cgcggcccat
     2101 ggcaacacgc tgacgctgac caccggagag ggccttcggc ttgcgctcaa ggtagggctc
     2161 caggtcgagg atcttggcgg cctcgaggac gcgcttggcg atctcgtcct tggagacgcc
     2221 ggcgatcttc agggcgaagc ccatgttgtc ggcgaccgac atgtgcgggt agagcgcgta
     2281 gttctggaag accatggcga cgtcgcggtc cttgggggac aggtgggtga
//
LOCUS       DAORPW010000294         2315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_17488_length_2315_cov_790.803982, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000294 DAORPW010000000
VERSION     DAORPW010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17488_length_2315_cov_790.803982"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..907)
                     /locus_tag="PLB86_13485"
     CDS             complement(29..907)
                     /locus_tag="PLB86_13485"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HRC66205.1"
                     /translation="MATLAQWVAGARPRTLPAAVSPVLVGTGAAYAVGRGDAGLAVLA
                     LAVALSLQVAVNYANDYSDGVRGTDAERVGPVRLVGQGLAVPGEVKLAAYLGFFLAAL
                     FGLALVALSQIWIFIPIGIACIIAAWRYTGGEHPYGYRGLGEVMVFVFFGLVATLGTQ
                     VTQAGGLTWAGLLGAIGIGCLASAILVANNLRDIPSDTVAGKHTLAVRLGDARTRRLW
                     TGLVGVAFAVAVGCAVWRVGSLVALLAVGLAVGPWRTLRSGAVGRALVPVLVATGRLQ
                     LGYAVLLALGLALTPR"
     gene            complement(1016..2206)
                     /gene="menE"
                     /locus_tag="PLB86_13490"
     CDS             complement(1016..2206)
                     /gene="menE"
                     /locus_tag="PLB86_13490"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HRC66206.1"
                     /translation="MRLQPIPLPFGPALLAAVGPLAAVLDGSGPPLLPYAAGSPPPVL
                     PPVTDADLPDDLALVVSTSGSTGIPKRTMLTANNLLASASAAHDVLSGPGSWLLALPA
                     HHIAGLQVIVRAALGSAGEPAVMDLTDGFTVEGFVAAALAMPPTSDPTYVSLVPTQVA
                     RLLADEAGIETLAAFDAVLCGAAAIPAAVRTRAHDLGIHLVTTFGMTETSGGCVYDGK
                     PLPVSEVHIDNDRHVVLGGATVAAGYLGDPGMTADHFTDDADGVRWFRAEDLGYLDDN
                     GRLHVTGRADEIINTGGVKVAPGPVEDALVRYLPGVRDAVVVGVPDPTWGQVVAAAVT
                     LLPSAGSARSAGVPTVEDARAALRGILPDAALPRLLQVVDAFPAKGPGKPDRAAISAS
                     FAPS"
BASE COUNT          388 a          783 c          879 g          265 t
ORIGIN      
        1 tacctcgacc cggcaggggt cagcggggtc agcgaggggt gagggccagg ccgagagcca
       61 gcaggacggc ataccccaat tgcaggcgtc cggtcgcgac caggacgggc acgagcgccc
      121 gcccaaccgc acccgagcgc agcgtccgcc acgggccgac cgcgagcccg acggccagca
      181 gcgcgacgag cgaccccacc cgccagaccg cgcacccgac ggcgaccgca aacgcgacgc
      241 ccacgagccc cgtccacagc cgacgcgtcc gggcatcacc caagcgcacc gcgagcgtgt
      301 gtttgccggc caccgtgtcg gaggggatgt cccgcaggtt gttggcgacg aggatggccg
      361 acgccaggca cccgatgccg atcgcgccga gcaacccggc ccacgtgagg ccgcccgcct
      421 gggtgacctg cgtcccgagg gttgcgacca acccgaagaa gacgaacacc atgacctcgc
      481 cgagcccgcg atagccgtag gggtgctcgc cgccggtgta gcgccaggcc gcgatgatgc
      541 aggcgatgcc gatggggatg aagatccaga tctgggacag ggcgacgagg gccagcccga
      601 agagcgcggc caggaagaac ccgaggtatg ccgcgagctt cacttcgccg ggaacagcca
      661 gtccctgccc gaccaggcgc accggcccga cccgctcggc gtcggtgccg cggactccgt
      721 cggaatagtc gttggcgtag ttgaccgcta cctgcaggga caacgccacg gccagcgcga
      781 gcacggccag gccggcgtca ccccgcccaa cggcatacgc cgcgccggtg cccacgagga
      841 cgggggagac ggcggcgggg agtgtgcgcg ggcgggcacc ggcgacccat tgggcgagcg
      901 tggccatgac ggtgagaggt ccttcggagc gtgaggcgag tgggcggtga gcttgctagg
      961 cccgcggcgt cgatgcggcg ggtggcgcag tgtgcgcctc caccctacgg gcggctcacg
     1021 acggcgcgaa ggaggcgctg atcgcggcgc ggtcgggttt gcctggaccc ttggccggga
     1081 aagcgtcgac aacctgcaac agtcggggca gggcggcgtc ggggaggatg ccacgcaagg
     1141 ccgcccgggc gtcctccacc gtggggacgc cagccgagcg tgcacttccg gccgagggca
     1201 gcagggtgac cgcggcggcg acgacctggc cccacgtcgg atcggggaca ccgacaacga
     1261 cggcatcgcg cacgcccggc aggtagcgca cgagggcgtc ctcgaccggg cccggggcga
     1321 ccttgacgcc acccgtgttg atgatctcgt cggctcgacc ggtcacatga agtcggccgt
     1381 tgtcgtcgag atagcccagg tcctcggcgc ggaaccaccg caccccatcg gcgtcgtcgg
     1441 tgaagtggtc ggcggtcatg ccggggtcac cgagatagcc ggccgcgacc gtcgcgccgc
     1501 cgaggacgac gtggcggtcg ttgtcgatat ggacctcgct gaccggcagc ggcttcccgt
     1561 catagacgca accgcccgag gtctcggtca tcccgaacgt cgtcaccagg tggatgccga
     1621 ggtcgtgggc gcgggtgcgc accgcagccg ggatcgccgc agccccgcag aggacggcat
     1681 cgaaggcggc cagggtctcg atgccggctt cgtcggctag caaccgagcc acctgggtcg
     1741 ggacgagcga cacataggtc gggtcggagg tggggggcat cgcgagcgca gcggcgacga
     1801 aaccctcgac cgtgaaaccg tcggtcagat ccatcacggc cggttcaccc gcggacccca
     1861 acgcggcccg gacgatgact tgcaggccgg cgatgtggtg ggcgggcagg gccagcagcc
     1921 atgagccagg cccggacagg acgtcgtgcg ccgcgctggc cgatgcgagc aggttgttgg
     1981 cggtgagcat ggtccgcttg gggatccccg tcgaccccga cgtcgacacg acgagcgcga
     2041 ggtcgtcggg aaggtccgcg tccgtcaccg gcggcagcac cgggggaggt gagcccgcgg
     2101 catacgggag cagcggtggc cccgacccgt cgaggacggc cgcgaggggg cctaccgcag
     2161 ccaggagcgc gggtccgaaa ggcaggggga tcggctgcaa tcgcatgagg tatgccgggt
     2221 cagaagtacc acgggaaggc ggaccagtcg gggtcgcgct tttgcaggaa ggagtcgcgg
     2281 ccttcgacgg cctcgtcggt catgtaggcg agccg
//
LOCUS       DAORPW010000295         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_17537_length_2312_cov_838.756314, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000295 DAORPW010000000
VERSION     DAORPW010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17537_length_2312_cov_838.756314"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLB86_13495"
     CDS             complement(<1..827)
                     /locus_tag="PLB86_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HRC66207.1"
                     /translation="MSTTPLIHLDAGQVRQARSLARRAGAPVVKLAKAHTTVSVERAT
                     LRLAGLTGADHEGQPWVNHLVDAVRGQVGLEHGVTTPVFDALRAGAAPDLLTLAQQSA
                     AGSVTFRLPEGRDLKATQRAARTAVARGIRTIDAQRAVRDRLIRRHGDPKQTPWIYLI
                     VATGDIDEDIPQAQAAAREGADVIAVIRSTGQSLLDFVPEGATHEGFAGTYATQENFR
                     LMRAALDETSREVGRYVRLTNYASGLCMPEIATLAGLERLDMMLNDSMYGILFRDINP
                     "
     gene            complement(929..2044)
                     /locus_tag="PLB86_13500"
     CDS             complement(929..2044)
                     /locus_tag="PLB86_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HRC66208.1"
                     /translation="MNQSVTSTVPASPVGLHRVLEPSGAAVTLPQAARRLDARREIWP
                     SEVRIAIDTLNLDAASYRQLAEKHRGVDGEVDGGAVREEVLAIVAERGKMQNPVTGSG
                     GMLIGTVDEVGPDSPLGLTVGDRVATLVSLSLTPLTITDGLRRWDGRSEQVPAAGHAV
                     LFERSIAAVIPDDLDPRLSLMVMDVCGAPALVSRVVGEYAARRVAGTGAGEESSAPSV
                     LVLGGAGKSGSLSLAAAREAGAGRLVAVVPNDREAQVLRPTGLADEVVIADARSPMGL
                     LAAVQAAGGPCDVTVVCVDVPGCEQPAILATAQEGTIIFFSMATSFAAAALGAEGLAA
                     DVRMIVGNGYVPGHAAYAMGLVRRNAAVRALFESRIE"
     gene            2128..>2312
                     /locus_tag="PLB86_13505"
     CDS             2128..>2312
                     /locus_tag="PLB86_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRC66209.1"
                     /translation="MTPVLEQPYAYRMRALVEPDWTRLPGWRDVTEAQWRDAQWQRSH
                     CVKNVKQLHELMGDLLT"
BASE COUNT          368 a          788 c          808 g          348 t
ORIGIN      
        1 gggttgatgt cgcggaagag gatgccgtac atcgagtcgt tgagcatcat gtccagccgc
       61 tcgagccccg cgagggtggc gatctcgggc atgcacaggc cggacgcgta gttggtcagc
      121 cggacatagc ggcccacctc gcggctggtc tcgtcgaggg cggcccgcat gagccggaag
      181 ttctcctggg tggcgtaggt gccggcgaag ccctcgtgag tggcaccctc gggcacgaag
      241 tcgagcaggc tctgccctgt cgagcggatg accgcgatga catcggctcc ctctcgggcc
      301 gcggcctgcg cctgcgggat gtcctcgtcg atgtcgccgg tggcgacgat gaggtagatc
      361 cacggggttt gcttcgggtc accatggcga cggatcagcc ggtcgcggac cgcgcgttgg
      421 gcgtcgatgg tgcgtatgcc gcgtgccacc gcagtgcgtg cggcccgctg ggtggccttg
      481 aggtcgcggc cttctggcag ccggaacgtc actgagccgg cggccgactg ttgagccagg
      541 gtgagcaggt caggagcggc ccctgctcgc agcgcgtcga agaccggagt ggtgacaccg
      601 tgctcaagcc cgacctggcc acgcacggca tcgacaagat ggttgaccca cggctggccc
      661 tcgtggtcgg ctccggtgag cccggccagg cgcagggtcg cccgctccac tgagaccgtg
      721 gtgtgcgcct tggcgagctt gaccactggt gcgccagcgc ggcgcgcgag tgacctggcc
      781 tggcggacct ggccggcatc caggtggatc agaggggttg tcgacacggt ctcagcgtag
      841 cagagacggg catttttcct gcgaaatgac agtcatgccg gagatctcac ggtccaaggg
      901 cgttacggag atcatccttg gcgtctactc actcgatgcg ggactcgaac agagcccgca
      961 ccgccgcgtt gcgacggacc agtcccatcg cgtatgccgc gtgccccggc acgtagccgt
     1021 tgcccacgat catccgcaca tcggcggcca acccttcggc gcccagcgcc gcggctgcga
     1081 aggacgtggc catcgagaag aagatgatcg tcccctcctg cgcggttgcc aggatggccg
     1141 gctgctcaca ccccggcacg tcgacgcaga ccacggtgac gtcgcacggt ccacccgccg
     1201 cctggaccgc agccagcagg cccatcggcg agcgggcatc ggcgatgacg acctcgtcgg
     1261 ccagccccgt cggacgcagc acctgcgcct cacggtcgtt cggaacgacg gcaaccagtc
     1321 ggccggcccc agcctcacgc gcggcggcca gggacagtga gccagacttg cctgctccgc
     1381 cgagaacgag gaccgacgga gccgacgact cctcaccggc accggtgccg gcgacgcggc
     1441 gagcggcata ctcccccacc acgcgggaca cgagcgcagg cgcgccgcag acgtccatga
     1501 ccatgagcga gaggcgcggg tcgaggtcgt ccgggatgac ggcggcgatc gagcgctcga
     1561 agaggacagc gtgaccggcg gccgggacct gctcggaccg gccgtcccat cggcgcagcc
     1621 cgtcggtgat cgtcagcggg gtgagcgaga gagacaccag cgtcgcgacc cggtcaccga
     1681 cggtgagccc gagggggctg tcggggccga cctcgtcgac cgtgccgatg agcatcccgc
     1741 ctgaccccgt gacggggttc tgcatcttgc cgcgctcggc gacgatcgcg aggacctcct
     1801 cgcggaccgc tcccccgtcg acctcgccgt cgacgccccg gtgcttctcg gcgagttgcc
     1861 ggtaggacgc ggcatcgagg ttgagggtgt cgatggcgat gcggacctcg ctgggccaga
     1921 tctcccggcg ggcatccagt cggcgggccg cctggggcaa cgtgacagca gcgccgctcg
     1981 gctcaagcac ccgatgcagc ccgaccggcg acgccggaac cgtggaagtt accgactggt
     2041 tcacttactg ctcctctcga ccatcgacgg cggaggattt atcgcgtcga atggctaaca
     2101 ccgtagatct tcctgtatct tagaggcatg acgcccgttc tggaacagcc ctacgcctac
     2161 cgcatgcgtg ccctggtcga gcccgactgg actcgcctgc ccggctggcg cgacgtcacc
     2221 gaggcccagt ggcgcgatgc ccagtggcag cgcagccact gcgtgaagaa cgtcaagcag
     2281 ttgcatgagc tcatgggtga cctgctcacc ga
//
LOCUS       DAORPW010000296         2309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_17571_length_2309_cov_881.799911, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000296 DAORPW010000000
VERSION     DAORPW010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17571_length_2309_cov_881.799911"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..871)
                     /gene="folP"
                     /locus_tag="PLB86_13510"
     CDS             complement(65..871)
                     /gene="folP"
                     /locus_tag="PLB86_13510"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRC66210.1"
                     /translation="MGVVNVTPDSFSDGGQWFEPDAAITHARGLLADGADLLDIGGES
                     TRPGASRPSVHEELSRVIPVVSALAAEGARVSIDTMRAEVAGAALAAGAALVNDVSGG
                     LADPQMLPLVAAAQVPFVVMHWRGHSVDMQDRAAYADVVAEVCRELASRRDAALAAGI
                     RADRLVLDPGLGFAKLAEHNWALLAGLPALRDLGQPILLGASRKAFLGKVGRPGHATP
                     RPAAERDVATAATTVLAGLAGVWAVRVHNVRSTRDALDVLEATQAHGGTP"
     gene            complement(958..1530)
                     /gene="folE"
                     /locus_tag="PLB86_13515"
     CDS             complement(958..1530)
                     /gene="folE"
                     /locus_tag="PLB86_13515"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRC66211.1"
                     /translation="MPVDHPRAQAAVRELLCAIGEDPDREGLRETPARVARALGEFFA
                     GLDQRPEDILSTTFDIDHDEMILVRDIEVYSTCEHHLVPFHGVAHVGYIPAKDGKVTG
                     LSKIARLVDVFAKRPQVQERLTTQVADALVDHLGVQGCIVVLECEHLCMSMRGVKKPG
                     SRTITSAVRGQLRNPATRAEAMALLLGGKR"
     gene            complement(1536..>2309)
                     /locus_tag="PLB86_13520"
     CDS             complement(1536..>2309)
                     /locus_tag="PLB86_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HRC66212.1"
                     /translation="AAAMNHTDPVTKVTILPRGRALGYTMVMPIDDKYSTTRNEILDQ
                     LAYALGGRVAEEMVFHDPTTGAANDIEKATGMARKMVTEFGMSERIGAIKLGQSTGEV
                     FLGRDMGHQRDYSEQVAGVVDEEVRKLIENAHDEAWHVINDNRDILDKLVLDLLEKET
                     LGTKELAEIFAPLVKRPLRPTWLSSDARAISERPPVLTPAETAASSNGHVPSMPPPGS
                     APQDQGAAAAGYPPPVGEPTSPSGQAGSGSTGGPAPQGH"
BASE COUNT          316 a          796 c          845 g          352 t
ORIGIN      
        1 cgctcgtgct cgaacacccc gtgatagccc aggccccgga tgccttcgac gacgatgcgg
       61 tcggtcatgg ggtgcctccg tgggcttggg tcgcttcgag gacatccaac gcgtccctcg
      121 tcgagcggac gttgtggacc cgcacggccc acacgccagc gagccccgcc agcaccgtgg
      181 tcgccgcggt ggcgacgtcg cgctcggcgg ccggcctcgg ggtcgcatgc ccgggccgcc
      241 cgaccttgcc gaggaaggcc ttccgggagg ccccgagaag gatcggttgc ccgaggtcgc
      301 gcagcgcggg caggccagcg agcagcgccc agttgtgctc ggcgagcttg gcgaagccca
      361 gcccggggtc gagcacgagc cggtcggcgc ggatccccgc ggcgagagcg gcgtcgcgac
      421 gggaggcgag ctcgcggcat acctccgcga cgacgtcggc gtatgccgcg cggtcctgca
      481 tgtcgacgct gtgccctcgc cagtgcatga cgacgaacgg aacctgcgcc gccgcgacga
      541 gcggcagcat ctgcgggtcg gcgagccccc cgctgacatc gttgacgagc gccgctccgg
      601 cggcgagcgc agcgcccgcg acctccgccc gcatcgtgtc gatcgacact cgggctccct
      661 cggcggccag cgccgacacg acgggaatca cccgactcaa ctcctcgtgg acgctgggac
      721 gcgacgcccc cggccgggtg gactcacccc cgatgtcgag caggtcggcc ccgtcggcga
      781 gcaggccgcg tgcgtgcgtg atcgccgcgt cgggctcgaa ccactgaccg ccgtcgctga
      841 acgagtcggg ggtgacgttg acgacgccca tgaccaacgt tcgggcgccg tcggagaacg
      901 gcccaggggt atgccgcccg ctcaccgagg ccagccactg cgagggtgcg ggggcggtca
      961 ccgcttcccg ccgagcagca gcgccatggc ctccgcacgg gtggcggggt tgcgcaactg
     1021 gccgcgcacc gccgaggtga tcgtgcggga tccgggcttc ttgacccccc gcatggacat
     1081 gcacaggtgc tcgcactcga ggacgacgat gcagccctgg acgccgaggt gatcgacgag
     1141 ggcatcggcg acctgcgtcg tgagccgctc ctggacctgc ggacgcttgg cgaacacgtc
     1201 gacgaggcgg gcgatcttgc tcagccccgt gaccttcccg tccttggcgg ggatgtagcc
     1261 cacatgggcg accccgtgga agggcacgag gtggtgctca cacgtgctgt agacctcgat
     1321 gtcgcgcacg aggatcatct cgtcgtggtc gatgtcgaag gtcgtcgaga ggatgtcctc
     1381 cggacgctgg tcaaggcccg cgaagaactc gccgagagcc cgagccaccc tggccggtgt
     1441 ctcgcgtagg ccttcccgat cggggtcctc gccgatggca cagagcaact cgcgcacggc
     1501 ggcctgcgcg cgcgggtgat cgaccggcat acggctcagt gcccctgtgg ggcgggaccg
     1561 ccagtgctgc cggaacccgc ctgccccgag ggactggtcg gctcgccaac gggcggcggg
     1621 taaccggccg cggccgctcc ctggtcctgc ggtgcgcttc cgggcggcgg catcgagggc
     1681 acgtgaccgt tgctcgacgc agccgtctcg gcgggcgtga gcaccggcgg gcgctcgctg
     1741 atggcgcggg cgtcgctgga cagccacgtc gggcgcagcg ggcgcttgac gaggggcgcg
     1801 aagatctcgg cgagctcctt ggtgcccagg gtctccttct cgaggaggtc caggacgagc
     1861 ttgtcgagga tgtcgcggtt gtcgttgatg acgtgccagg cctcgtcgtg agcgttctcg
     1921 atgagcttgc gcacctcttc gtcgacgacg ccggcaacct gctcggagta gtcacgctgg
     1981 tggcccatgt cgcggccgag gaagacctcg cccgtgctct gaccgagctt gatggcgccg
     2041 atccgctcgc tcatcccgaa ctcggtgacc atcttgcggg ccatcccggt ggccttctcg
     2101 atgtcgttgg ccgcgccggt ggtcgggtcg tggaagacca tctcctcggc gacccggccg
     2161 ccgagggcgt aggcgagttg gtcgaggatc tcgttgcgag tggtggagta cttgtcgtcg
     2221 atcggcatga ccatcgtgta accgagggcc cggccgcgcg gcaggatcgt caccttggtc
     2281 acggggtcgg tgtggttcat cgccgcggc
//
LOCUS       DAORPW010000297         2281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_17936_length_2281_cov_626.230458, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000297 DAORPW010000000
VERSION     DAORPW010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17936_length_2281_cov_626.230458"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            538..729
                     /locus_tag="PLB86_13525"
     CDS             538..729
                     /locus_tag="PLB86_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66213.1"
                     /translation="MSALSRNHLLALAAIGLALFVVAIGFAAAKAEVSPTVLVVSALV
                     AAAAGGAVIAAILRGPGRS"
     gene            complement(839..2266)
                     /locus_tag="PLB86_13530"
     CDS             complement(839..2266)
                     /locus_tag="PLB86_13530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC66214.1"
                     /translation="MFDPYAAADPAVVAASLADAVPEVYWTDPAQRPMRPDPRPVLHG
                     TSHRADLVVIGGGFTGLWAALQALEDDPSATVVVLEAGHLGTGASGRNGGFVSASLTH
                     GLAQGVALWADELPTLERLGTDNIAGLEASLTAYGIDADFHRPGELTLSLTPNQDAAI
                     TEGYELHRAHGLAGEVLDADAARARVNSPLYRGGWFDPSVALVDPARLVWGLAAAVES
                     LGGTIHEGSPATSLEVDGAGVRVTTTSGAIAARRVVVGTNAYPGVLKRLRPFILPVYD
                     HVLMTEPLTAAHLSAIGWQGREGLTDAGNQFHYYRRTLDDRVLFGGFDANYHFRGRID
                     PALEQSASHDLLARHFFAIFPQLAGLRFTHRWAGVIDTTSRFTPFFGRTHGGRVAYAV
                     GYTGLGVGSSRFGARVALDLLRGRDTERTRLAMVRRKPVPFPPEPIRYAAVRATRASL
                     AAEDRTGQRNFWLRTLDRLGVGFDS"
BASE COUNT          323 a          737 c          878 g          343 t
ORIGIN      
        1 accggcgggt ctggtgcccg aagtggacgc cgctctcgag gagctggcgc atggtaacga
       61 cggccatgcc gtgttctcct atgtgtcgtt gtcagttacg tcggtaagcc gcggggtccg
      121 cgcacgaggc gcgggtgcgg catacctgcc tggcgtcctg acccaccccg ccggtatgcc
      181 gtgtggccga ccggactgct gtggtgcgcc ttcggtggtc atgggaccgc cgacgaggga
      241 cgcgcgaaat ggtgctgcga tggtgccgat ggcccggagg ccatcggcca gtctacgtcc
      301 tgggccgggt ccgccggtaa tcgccgtgcc acgccccgtg tatcgaagct gcgcggctca
      361 gctgtcgaac ccgacccgcg ccggtcgtgg ctgggcgcgc cgtacaccgg gtgacacact
      421 cgtcgtaggg catcaacctg ccatggcacc aaggtgtcag ccgttccccc tttgtcaatc
      481 tccgacccgc ccatagcgtc cttgtgggta gcggcgcttc gggcccgagg aggagttgtg
      541 tctgcgcttt ccaggaacca cctgctcgca ttggcggcca tcggattggc cctgttcgtg
      601 gtggcgatcg ggttcgccgc agccaaggcg gaggtttcgc caaccgtcct cgtggtctcc
      661 gccctcgtcg ccgctgctgc cggcggggca gtgatcgcgg ccatcctgag gggcccaggc
      721 cgtagctgag ccctcctccc cgaccaacgg cctgaagcga gttgctggct gcgtcaattc
      781 ctgaggagcc aggccgacgc cggtcgctcc gtgttgttgt cgagccacct ctaggcgatc
      841 aactgtcgaa gccgacgcca agccggtcga gggtgcgcag ccagaagttg cgctgtccgg
      901 tgcgatcttc ggcggcgagg gatgcgcgcg tggcgcggac cgcggcatac cggatcggct
      961 cgggcgggaa cggcacgggc ttgcgccgga ccatcgccag ccgggtgcgc tcggtgtcgc
     1021 gccctctgag cagatccagc gcgacccggg caccgaagcg cgaggagccg accccgagtc
     1081 cggtgtagcc gacggcatag gcgacccggc cgccgtgggt gcggccgaag aacggggtga
     1141 agcgggaggt cgtgtcgatg acgccggccc agcgatgggt gaaccgaagc ccagccagtt
     1201 gggggaagat cgcgaagaag tgcctggcga gcaggtcgtg agaggcggac tgctccaggg
     1261 ccgggtcgat ccggccgcgg aagtggtagt tggcgtcgaa gccgccgaag aggacgcggt
     1321 cgtccagggt gcgccggtag tagtggaact ggttgcccgc gtcggtgagg ccttcgcggc
     1381 cctgccaccc gatggcgctg aggtgggcgg cggtgagcgg ctcggtcatg aggacgtggt
     1441 cgtagacggg gaggatgaag gggcgcagtc gcttgagcac gcccgggtag gcgttggtcc
     1501 cgacgacgac ccgtcgggcc gcgatggcac cggaggtggt cgtcacccgg accccggcgc
     1561 cgtccacctc caacgaggtg gccgggctgc cctcgtggat cgtcccgccg agcgactcga
     1621 ccgccgcggc cagcccccac acgaggcggg ccgggtcgac gagggccacc gacgggtcga
     1681 accagccgcc ccggtagagc ggtgagttga cccgggcgcg ggctgcgtcg gcatccagga
     1741 cctccccggc caggccgtgg gcacggtgca actcatagcc ctcggtgatc gccgcgtcct
     1801 ggttgggggt gagcgagagg gtcagctcgc cggggcggtg gaagtctgcg tcgatcccgt
     1861 atgccgtgag gctggcttcc agcccggcaa tgttgtcggt gccgagccgc tcgagggtgg
     1921 gcagttcgtc ggcccagagg gcgacgcctt gggccaggcc gtgcgtgagc gacgcggaga
     1981 cgaagccgcc gttgcgaccg gaggccccgg tccccaggtg gccggcttcc agaacgacga
     2041 cggtggcgct ggggtcgtcc tccaaggcct gcagggcggc ccagaggccg gtgaagccac
     2101 cgccgatgac gaccaggtcg gcccgatgtg aggtgccgtg caggacgggt cgggggtccg
     2161 ggcgcatcgg gcgctgggcc ggatcggtcc aatagacctc gggaacggcg tcggcgaggg
     2221 acgccgcgac gaccgcgggg tcggcggcgg catacgggtc gaacacctcg ggcgcgactg
     2281 a
//
LOCUS       DAORPW010000298         2280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_17951_length_2280_cov_619.041348, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000298 DAORPW010000000
VERSION     DAORPW010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17951_length_2280_cov_619.041348"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..862
                     /locus_tag="PLB86_13535"
     CDS             131..862
                     /locus_tag="PLB86_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66215.1"
                     /translation="MNVKKISIAAASVAAVAALAIGGSAIANAADNPTPTTTSSATST
                     QGTADSGTTGTQGKGQRGGTGATGQQGSQDTPVTGDEAAKVSAAVTAKDSTVTVTSVR
                     KDPDGSYDVLGTKAGANVMFDVSADLATITENAGGPGGGKGGGMGGASQDTPVTGDEA
                     AKVSAAVTAKDSTVTVTSVRKDPDGSYDVLGTKAGANVMFDVSADLATITENAGGPGG
                     MGGGKHGAPGAADSTQTAPSATSSS"
     gene            complement(965..1285)
                     /locus_tag="PLB86_13540"
     CDS             complement(965..1285)
                     /locus_tag="PLB86_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRC66216.1"
                     /translation="MTRPERGEVWWCEPPDIGRRPVVVLSRSAAIIGRGRALVAPCTT
                     TVRGLPSEVALDPRTDPVPRRCVATLDAVESASLGVLVERLGRLSDTRMREMCAALAV
                     AVDC"
     gene            complement(1282..1521)
                     /locus_tag="PLB86_13545"
     CDS             complement(1282..1521)
                     /locus_tag="PLB86_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE5"
                     /protein_id="HRC66217.1"
                     /translation="MAKIRVSTTVDATLLAQVRALDPTGTDASLLERAFTALLASQRR
                     AEVDAAYREAYAAHPADEPDAWGDLATFGAAVARS"
     gene            1609..2121
                     /locus_tag="PLB86_13550"
     CDS             1609..2121
                     /locus_tag="PLB86_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRC66218.1"
                     /translation="MAHTLVVIRHAKSDWDVDAADRDRPLAKRGRRQAPPTGRWLAAQ
                     DLALDLAVVSPAARARQTWELVAAQLDRPPTTRVEEAAYTFDGDELLEVVRALPESAG
                     AVVLVGHNPAVEEFVETLTGEYAALPTSAVAVVEVASWAGVGPGTGRLLANLRPSDAE
                     LPDTELPGTD"
BASE COUNT          359 a          823 c          813 g          285 t
ORIGIN      
        1 ctagcgggga gcggcgcgag caggtcgtag ccaaccccca cctgcgtgac aggttggtca
       61 taggtgggca cccgagtctt ctcatcagca gaggtcgtca agaggcctcg acccgacaag
      121 gagaagcccg atgaacgtca agaagatcag catcgcagcc gcatcagtcg ccgccgtcgc
      181 cgccttggcg atcggtgggt ccgcgatcgc gaacgccgcc gacaacccga ccccgacgac
      241 cacctcctcg gccacgtcca cgcagggcac cgccgactcc ggcaccacgg gcacccaggg
      301 caagggccag cgcggcggca ccggtgccac cggtcagcag ggctcgcagg acaccccggt
      361 gaccggtgac gaggccgcga aggtctcggc tgcggtcact gccaaggact cgaccgtcac
      421 ggtgacctcg gtccgtaagg accccgacgg ttcctacgac gtgctcggca ccaaggccgg
      481 cgccaacgtg atgttcgacg tcagcgccga cctggccacg atcaccgaga acgccggcgg
      541 ccccggcggt ggcaagggtg gcggcatggg cggagcttcg caggacaccc cggtgacggg
      601 tgacgaggcc gcgaaggtct cggccgcggt gaccgccaag gactcgacgg tcacggtgac
      661 ctcggtccgt aaggaccccg acggttccta tgacgtgctg ggcaccaagg ccggcgcgaa
      721 cgtgatgttc gacgtcagcg ccgacctggc gacgatcacc gagaacgccg gtggccccgg
      781 cggcatgggc ggcggcaagc acggcgcccc cggcgcggcc gactccaccc agaccgctcc
      841 gagcgccacc tcctccagct gacccgaccc gcgcgcggac tcccgcaccc gcgcgcggca
      901 tacccgcaag gcccgtgacc caccccctgg ggtcacgggc ctttgcgtat gccgcctcgt
      961 cccgttagca gtcgacggcc accgcgagcg ccgcgcacat ctcgcgcatc cgggtgtcgg
     1021 acaatcggcc gagccgctcg acgagcaccc cgagggatgc cgactcgacg gcgtcgaggg
     1081 tggcgacaca gcggcggggc actgggtcgg ttcgcgggtc gagcgccact tcgctgggca
     1141 agccgcgcac ggtcgtcgtg cagggagcga cgagcgctcg cccccgcccg atgatcgccg
     1201 cggagcggga gagaacgacg accgggcgcc gcccgatgtc cggtggctcg caccaccaca
     1261 cctcgccgcg ctccggccga gtcatgagcg ggccaccgcg gcgccgaacg ttgccaggtc
     1321 gccccacgca tccggctcat cggcggggtg agcggcatac gcctcccggt atgccgcatc
     1381 gacctcggcc cggcgctggc tcgccaacaa cgcggtgaag gcccgttcca ggaggctggc
     1441 gtccgtgccg gtcggatcca gcgcccggac ctgggccaac aacgtggcgt ccacggtggt
     1501 gcttacccgg attttggcca tgccacaact atgccacgcc tacagcacga tcatcgtgcc
     1561 gctctggggt tagggtgagc cagccacgtg cccacaaagg gaggacccat ggcccacacg
     1621 ctcgtcgtca tcaggcacgc gaagtccgac tgggacgtcg atgccgccga ccgggaccgg
     1681 ccgctggcca agcgtgggcg ccggcaggct ccgccgactg ggcgctggtt ggccgcgcag
     1741 gacctcgcgc tcgacctggc ggtcgtctca ccggcggcca gggctcgcca gacctgggag
     1801 ctcgtcgcgg cgcagctcga ccggccgccg accacgcgcg tggaggaggc ggcatacacc
     1861 tttgacggcg acgagttgct cgaggtggtg cgggcgttgc cggagtcggc gggcgcggtc
     1921 gtcctcgtgg gacacaatcc ggcggtcgag gagttcgtgg agacgttgac cggtgagtat
     1981 gccgcgctgc ccacctccgc ggtcgcggtc gtcgaggtgg cgtcctgggc gggcgtcggg
     2041 ccggggactg gtcggctgct ggccaacctg cgtccctcgg acgccgagct gcccgacacc
     2101 gaactgcctg gcaccgacta gctgatagcc gggcggcggc ggggctcaat cagcggcgcg
     2161 gaaggcctcg gtggcgagga actctcctgc ggcagagcac gcgagcacct cgatgtccca
     2221 gcgaacgagg gcgtgctcga gggtgcggcg gccaccggcg aggatcgccg tcgccaggac
//
LOCUS       DAORPW010000299         2273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_18029_length_2273_cov_651.899910, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000299 DAORPW010000000
VERSION     DAORPW010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18029_length_2273_cov_651.899910"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..582
                     /locus_tag="PLB86_13555"
     CDS             7..582
                     /locus_tag="PLB86_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66219.1"
                     /translation="MAGTVPKHPRTVVLLEGASDVAAVRTLAAAREVNLERVRLVDLG
                     GVTNIRRVLRQARADWADAEVLGLCDAAEVRFIERALVEIGLPVRDPSDLPAYGFFVC
                     HADLEEELLRALGPVRTIAVIERLGLGAKLGALQRQLAWQDRPLAEQLHRFCGVASGR
                     KELLGGALAEALDLDAVPEPLALLLDRLADA"
     gene            complement(601..1854)
                     /locus_tag="PLB86_13560"
     CDS             complement(601..1854)
                     /locus_tag="PLB86_13560"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC66220.1"
                     /translation="MTAAPPDQPGPAPAGLGAGYWRMFAADSVSSIGTVVSTLAIQFL
                     LIDTLKADEQAIGLVRAAQWAPYLVFGLLAGVIAERVRRKPLLVAADLLGMTIFGAIG
                     LLALAGRLTVPIVAGLVFVFGAVTCVSVAAYQSYVPRLVPERALPDAFARLEQMGQVV
                     GSVGPVAAGSLVRFLSAPIAVLVDALSYAVSAVVIATIRTPEPPMVRGEARHLVVELR
                     EGARWVYRHETLRPYAFWLHSWFVGRAMVMTVLVFYANQELHLDALTVGLILAAAGVS
                     GVVAAGVAPRLGRRFGVGRVITTVEWVSPLWPVLVVLAQPGPWGVAVLVVAMLLDGLT
                     AMSASLMMTYRTAVTPDRLRARMNATIRTFNWGGLAIASFVSGWVASMWGSRVAMLAG
                     AAILLVATAYLRRSAFRAAVLPVAV"
     gene            complement(1851..>2273)
                     /locus_tag="PLB86_13565"
     CDS             complement(1851..>2273)
                     /locus_tag="PLB86_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRC66221.1"
                     /translation="VIRTANAFNAAAFHIVGRRRWNRRGAMVTDRYQHEHHHPDAAAL
                     ATWAQAQTEAGEAGGIPLIGIDNLPGSVALETYDLPRECVLVFGQEGPGLTPEMQQVC
                     RVVLHIDQFGSTRSINAGAAAAIAMHAWVRRHVFDQRP"
BASE COUNT          369 a          805 c          807 g          292 t
ORIGIN      
        1 gcgtggttgg ctggcaccgt gccgaagcac ccccggaccg tcgtcctcct tgagggggcg
       61 agtgatgtcg cagccgtccg caccctcgcc gctgcccgcg aggtcaacct ggaacgagtc
      121 cggctggtcg acctcggcgg ggtcaccaac atccgtcggg tgctgcgcca ggcccgagcc
      181 gattgggcag atgccgaggt gctcgggctc tgcgacgcgg cagaggtgcg tttcatcgag
      241 cgggccctcg tcgagatcgg gctgccggtg cgcgacccaa gcgaccttcc tgcctacggg
      301 ttcttcgtct gccacgccga tctcgaggag gaactgcttc gggccctcgg gccggtgagg
      361 accatcgccg tgatcgagag gctgggcctc ggggccaaac tcggtgcgct gcaacgacag
      421 ttggcctggc aggaccgtcc gctcgctgag caattgcacc gtttctgcgg ggtcgcgtcc
      481 ggacgcaaag agttgctcgg gggtgcgctc gccgaggccc tcgacctgga tgcggtgccc
      541 gagccgctcg ccctcttgct cgaccgcttg gcggacgcct gagcctcggc tcggtcgggc
      601 tcagaccgcg accggcagca ccgccgccct gaaggccgac cgacgcaggt atgccgtcgc
      661 caccagcagg atcgcggctc ccgccagcat cgcgacccga ctcccccaca tgctggccac
      721 ccaccccgac acgaaggacg cgatggccaa cccgccccag ttgaaggtgc ggatcgtggc
      781 attcatccgc gcccggagcc ggtcgggggt gactgcggtg cggtaggtca tcatcagcga
      841 ggcactcatc gcggtcagcc catccagcag catcgcaacg accaaaacgg cgacgcccca
      901 gggcccgggc tgtgcaagca cgacgagcac cggccacagc ggcgacaccc attcgacggt
      961 ggtgatgacc cggccgacac cgaaccgccg ccccagccgc ggcgccaccc cggcggccac
     1021 gacacccgac acgccagcgg cggccaggat gaggcccacg gtgagggcat ccaggtgcaa
     1081 ctcctggttg gcgtagaaga ccagcaccgt catcaccatg gcccgcccga cgaaccagct
     1141 gtgcagccag aaggcatacg gccgcagcgt ctcgtgccga tagacccacc gggcaccttc
     1201 gcgcagctca acgacgaggt gccgggcctc gccccgcacc atgggcggct ccggcgtgcg
     1261 gatggtcgcg atgacgacgg cggacacggc atacgagagc gcgtcgacga gcaccgcgat
     1321 cggcgcggag aggaagcgca cgagcgagcc cgcggcgacc ggcccgaccg agccgaccac
     1381 ctgccccatc tgctcgagac gggcgaaggc gtcggggagg gcgcgctcgg gcacgagcct
     1441 cgggacatag gactggtatg ccgcgaccga cacgcacgtg accgcgccga acacgaagac
     1501 cagcccggcg acgatgggga cggtgagccg gccggcgagg gccagcaggc cgatcgcccc
     1561 gaagatcgtc atccccagca ggtcggcggc cacgagcagg ggtttgcggc ggacccgttc
     1621 ggcgatcacg ccggcgagca gcccgaacac caggtaggga gcccactgcg cggcacggac
     1681 caggccgatc gcctgctcgt cggccttgag ggtgtcgatg aggaggaact ggatcgccag
     1741 ggtcgagacg accgtcccga tcgacgagac cgagtcggcg gcgaacatcc gccagtagcc
     1801 agccccgagc ccagcgggag ccgggcccgg ttggtcggga ggcgccgccg tcatggccgc
     1861 tggtcgaaga cgtggcggcg cacccaggca tgcatggcga tggctgccgc ggcaccggcg
     1921 ttgatcgagc gggtcgagcc gaactggtcg atgtgcagga ccacacggca tacctgctgc
     1981 atctcaggcg tcagcccggg cccctcttgg ccgaagacga ggacgcactc gcgcggcagg
     2041 tcgtaggtct ccagcgcgac cgagccgggc aggttgtcga tcccgatgag aggtatgccg
     2101 cccgcctccc ccgcctccgt ctgcgcctgt gcccacgttg cgagcgcggc ggcgtcgggg
     2161 tggtggtgtt cgtgctggta gcggtcggtg accatcgccc cgcgccggtt ccaccgtcgc
     2221 cgcccgacga tgtggaaggc ggcggcgttg aaggcgttgg ccgtgcggat cac
//
LOCUS       DAORPW010000300         2259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_18234_length_2259_cov_801.678312, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000300 DAORPW010000000
VERSION     DAORPW010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18234_length_2259_cov_801.678312"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLB86_13570"
     CDS             complement(<1..275)
                     /locus_tag="PLB86_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRC66222.1"
                     /translation="MVGTSEGEARALAALSAAGVAHEVTRHGPVGSLAEAAAARGVRP
                     ADIVKTLVVRRGDGDFLFVLVPGDRKFSWPKLRALLGTSRLSMPDADG"
     gene            480..1805
                     /locus_tag="PLB86_13575"
     CDS             480..1805
                     /locus_tag="PLB86_13575"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HRC66223.1"
                     /translation="MSSFWAEHVWLPSGLARGVRLVVDDGRFVVVEPRATRHPEDVKL
                     RGVVFPGLANAHSHVFQRALRGRTQSDADNGITWRAKMYDVAERLNPDLYLALARATF
                     AEMALAGITVVGEYHYLHHGPGGSRYSDPNAMGAALARAAHEVGIRLTLLDTLYLQGG
                     LTSEGHLPLDGVQQRFSDGSVEAWAARTARIEQTELLRRGAAIHSIRAVSREDAAYAA
                     QLVGDRPLHMYVSEQPAENLACQMYYGTTPTELLADIGVLGSEFTAVHATHLTERDIS
                     LLADHECQVVVCPTSEQDMADGVPAVRRLLDAGVPVGIGTDQHAFIDPFVEMRELEMH
                     ERLGSGERGRISTPQLLRCGSVHGYQSLGWYDGGSIAPGLVADFITVDLESTRTAGSK
                     AAEVIYTASAADVLTVVVGGRVIVENGQHAFGPVAPLMREALSLIRDAS"
     gene            complement(1839..2132)
                     /locus_tag="PLB86_13580"
     CDS             complement(1839..2132)
                     /locus_tag="PLB86_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HRC66224.1"
                     /translation="MGPMVWKVLGTGSAVLAATVAQKLVTKGWELASGKPAPDDPNHP
                     EDTTWKEAVLFAALTGLAVSAARVMATRKAAQYYAKSAGHLPAALVKADGATG"
BASE COUNT          337 a          837 c          739 g          346 t
ORIGIN      
        1 ccgtccgcgt cgggcatcga caaccgcgac gtgcccagca gcgcccgtag tttcggccac
       61 gagaacttcc ggtccccagg gacgagcacg aacaggaaat ccccgtcccc gcgccgcacc
      121 acgagcgtct tgacgatgtc agccggccgc accccacgcg ccgccgcagc ctccgccagg
      181 ctcccgaccg gcccgtgtcg cgtgacctcg tgcgcgaccc cggccgccga caacgcggcc
      241 aacgctcgcg cctcaccctc gctcgtgccc accatcgccc cagggtatgc cgcgtcgcgg
      301 tgccgccgtc gacgacgcag ccgccgccgt cgacgccgac gaagggttta aaagggggca
      361 ggtagtcccg gttgtcacat ctggacgcct ggcaatgtca ggatggaggc tgtcctcgac
      421 gggccacgtg gtggtcgggg caccgtcgag cacgatcgag tcgatcgaca aggagtgtcg
      481 tgtcgtcgtt ctgggccgag cacgtgtggc tgccgtcggg cttggcccgc ggcgtgcgct
      541 tggtcgtgga cgatggtcgg ttcgtcgtcg tcgagccccg agcgacccgc caccccgagg
      601 atgtcaaact ccgcggggtc gtcttccccg ggttggccaa tgcccatagc catgtcttcc
      661 agcgggccct gcgcggacgc acccagtctg acgcggataa cggaatcact tggcgcgcaa
      721 agatgtacga cgtcgctgaa cgcctcaacc ctgacctcta tctcgctcta gcccgggcca
      781 ccttcgccga gatggcgctg gcaggcatca ccgtggtcgg cgaataccac tacctgcacc
      841 acggtccggg cggctcgcgc tactccgacc ccaacgccat gggcgctgcc ctggccaggg
      901 cagcgcacga ggtcgggatc cgactgaccc tgctggacac cctctacctg cagggtgggc
      961 tcacctccga ggggcacctg ccgctcgacg gtgtgcagca gcggttcagc gacggcagcg
     1021 tcgaggcctg ggccgcccgc accgcccgca tcgagcagac cgaattgctt cgtcggggcg
     1081 cggcgatcca ctcgatcagg gccgtgtcgc gcgaggacgc cgcctacgcc gcgcaactcg
     1141 tcggggaccg tccgctgcac atgtatgtct ccgagcagcc cgccgagaac ctcgcctgcc
     1201 agatgtatta cggcaccacc ccgaccgaac tcctcgccga catcggcgtg ctcgggtcgg
     1261 agttcacggc cgtccacgcg acccacctca ctgagcgcga catcagcctc ctggccgacc
     1321 acgagtgcca ggtggtcgtc tgcccgacgt ccgagcagga catggccgat ggcgtcccgg
     1381 cggtccgcag gctcctcgac gccggggtcc cggtcggcat cggcaccgac caacacgcct
     1441 tcatcgaccc gttcgtcgag atgcgtgaac tcgagatgca cgagcgcctc ggcagcggcg
     1501 agcgtggccg gatctcgacc ccccaactcc tgcgctgcgg gtcggtccac ggctatcaga
     1561 gcctgggttg gtacgacggc gggtccatcg cccccggtct ggtcgccgac ttcatcaccg
     1621 tggatctcga gtccacccgc accgcaggca gcaaggccgc cgaggtcatc tacaccgcga
     1681 gcgccgcgga cgtcctcacc gttgtcgtcg gcggtcgggt catcgtcgag aacggccagc
     1741 atgcgttcgg cccggtcgcc ccgctcatgc gtgaggcgct gtcgttgatc cgcgacgcga
     1801 gctgagcgcg cgcggcatac gcgatccggt atgccgcgtc agccggtcgc gccgtcggcc
     1861 ttgaccaacg cggcgggcag gtgaccagcc gacttcgcgt agtactgggc ggccttgcgg
     1921 gtcgccatga cccgggccgc gctcacggcc agcccggtga gggcggcgaa gaggacggcc
     1981 tctttccagg tggtgtcctc gggatggttg gggtcgtcgg gggccggctt gccggaggcg
     2041 agctcccagc ccttggtgac gagcttctgg gcgacggtcg ccgccagcac tgccgagccg
     2101 gtgcccagca ccttccacac catcggaccc atcccgcacc tcctgctcca ccgggccact
     2161 cgccaacccg tgtcgttcgc ctcgtccgac tctggcacgg actcggggta aagtcatcat
     2221 cgccacgaca ggggagcgcc ccagcgctga gagtgcggt
//
LOCUS       DAORPW010000301         2236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_18555_length_2236_cov_1001.500229, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000301 DAORPW010000000
VERSION     DAORPW010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18555_length_2236_cov_1001.500229"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1618)
                     /gene="purF"
                     /locus_tag="PLB86_13585"
     CDS             complement(2..1618)
                     /gene="purF"
                     /locus_tag="PLB86_13585"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRC66225.1"
                     /translation="MAGGDGLLTHDLMPGEKGPQDACGVFGVWAPGEDVAKLAYFGLY
                     ALQHRGQESAGIATGDGHKVLVYKDMGLVSQVFNESALQSLTGHVAIGHCRYSTTGGS
                     TWENAQPTLGGTDTTTVALAHNGNLINSAELRDLVAERHGHLDRHPGELGRGNTTDTA
                     LVTALLSDPDRSLEAVALDVLPRLRGAFCFVFMDEHTLYAARDPHGIRPLALGRLDRG
                     WVVASETAALQTVGASVIREIEPGELVVIDEQGLRSHRFAEPAPKGCVFEYVYLARPD
                     AVINQRVVHEARVAMGARLAIEHPVDADLVIGVPDSGVPAAVGYAQASGIPFGQGFVK
                     NAYVGRTFIQPSQTLRQLGIRLKLNPLAHTIRGKRLVVVDDSIVRGNTQRAQVRMLRE
                     AGAAEVHVRISSPPVNWPCFYGIDFATRAELIATGLGVEEIRASIGADSLGYISLEGM
                     IEATNQPADRLCAACFTGTYPVDLPEDGRIGKHLFEPLPIDLSHSVRDVDPAPLSGAD
                     IGARAGGIRVDVEGVGLGVDGGAENALLHP"
     gene            complement(1655..1912)
                     /locus_tag="PLB86_13590"
     CDS             complement(1655..1912)
                     /locus_tag="PLB86_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66226.1"
                     /translation="MTSPGTPPPSQRRRRAPRLVPFIATGAILGYVVGLLLAARPVAD
                     EAAQRLQNYSFNSQAGYLGLFGALMGALLGAVVFVVLDRRS"
     gene            complement(1909..>2236)
                     /locus_tag="PLB86_13595"
     CDS             complement(1909..>2236)
                     /locus_tag="PLB86_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HRC66227.1"
                     /translation="VCRAAGFASAPREALATAVRFTLEELATLAPGRSVEVRVPPFGA
                     TQCVPGPVHRRGTPASVVETDAVTWLSVAVGDLGWAEALDSGAVRASGERAALGEWLP
                     LGRLSG"
BASE COUNT          358 a          811 c          771 g          296 t
ORIGIN      
        1 gtcaggggtg cagcagggcg ttctcggcgc caccgtcgac accgaggccc acgccttcga
       61 catcgactcg tatgccgccc gcgcgcgcgc cgatgtccgc ccccgacagc ggggccggat
      121 cgacgtcccg caccgagtgg gagaggtcga tgggcaacgg ctcgaacagg tgcttgccga
      181 tccgaccgtc ctccggcagg tccaccgggt aggtgccggt gaaacacgcc gcgcacagcc
      241 ggtcagccgg ctggttcgtg gcctcgatca tcccctccag ggagatgtag cccaacgagt
      301 cggcaccgat cgaggcccgg atctcttcga cccccaggcc agtggcgatg agctcggcgc
      361 gggtcgcgaa gtcgatgccg tagaagcacg gccagttgac cggcggcgag gagatccgca
      421 cgtggacctc ggccgccccc gcctcacgca gcatccggac ctgggcccgc tgggtgttgc
      481 cgcgcacgat cgagtcgtcg acgacgacca gccgcttgcc ccggatcgtg tgggccagcg
      541 ggttgagctt gagccggatg cccaactggc gcaacgtttg cgagggctgg atgaaggtcc
      601 gcccgacata ggcgttcttg acgaaaccct gcccgaacgg gatccccgac gcctgggcat
      661 aaccgacggc ggccggaaca ccggagtcgg gcaccccaat gaccagatcg gcatcgacgg
      721 gatgctcgat ggccaaccgc gcgcccatcg ccacgcgcgc ctcatgcacg acacgctggt
      781 tgatgacggc atcgggccgg gcgaggtaga cgtattcgaa cacgcacccc ttgggcgcag
      841 gctcggcgaa gcggtgcgac cgcagcccct gctcgtcgat gacgacgagc tcaccgggct
      901 cgatctcacg gatcaccgac gcgccgacag tctgcagggc agccgtctcc gacgccacga
      961 cccagccgcg gtcgagccga cccagcgcca gggggcggat gccgtggggg tcgcgggcgg
     1021 catacagggt gtgctcgtcc atgaagacga agcagaaggc gccccgcaac cgcgggagca
     1081 cgtccaacgc cacagcttcg agcgagcggt cggggtcgga gagcagtgcc gtcaccaggg
     1141 ccgtgtcggt ggtgttgccg cgccccagct ctccgggatg ccggtcgagg tgcccgtgcc
     1201 gttccgcgac gaggtcacgc aactcggccg agttgatgag gttgccgttg tgggccagcg
     1261 cgaccgtcgt cgtgtcggtg ccgccgagcg tgggctgcgc gttctcccag gtcgacccgc
     1321 cggtcgtcga gtagcggcag tggccgatcg cgacgtgccc ggtgagcgac tgcagcgccg
     1381 actcgttgaa gacctggctg accagcccca tgtccttgta gacgagcacc ttgtggccgt
     1441 cgccagtcgc gatgccggcg gactcctggc cgcggtgctg caacgcgtag agcccgaagt
     1501 aggcgagctt ggccacgtcc tccccgggtg cccagactcc gaagacccca caggcgtcct
     1561 gtgggccctt ctcccccggc atgagatcgt gcgttaacag cccgtcgcca cctgccacgt
     1621 gcgcaagtgt cccacaggta tgccgtgctg ccggtcagct cctgcggtcg agcaccacga
     1681 acacgacggc ccccaacagc gcgcccatga gcgcgccgaa cagcccgaga tacccggcct
     1741 gcgagttgaa cgagtagttc tgcagtcgct gggcggcctc gtctgccacg ggtcgggcgg
     1801 ccaacagcag cccgacgaca tatccgagga tggcgccggt cgcgatgaac ggcacgaggc
     1861 gcggggcgcg acggcgtcgc tggctcggcg gcggggtccc tggtgacgtc acccggacag
     1921 cctacccagc ggcagccact cgcccagcgc cgcccgctcc cccgacgcgc gcaccgcccc
     1981 ggagtcgagc gcctcggccc aaccgaggtc cccgaccgcc acagacaacc aggtcacggc
     2041 gtcggtctcg acgacggagg ccggggttcc ccgccggtgg acgggccccg gcacgcactg
     2101 ggtcgccccg aacggaggca cccgcacctc gaccgatcgt ccgggcgcca gcgtggccaa
     2161 ctcctcgagg gtgaaccgca ccgcggtcgc gagcgcctcc cggggggccg acgcgaaccc
     2221 tgccgcacgg catacc
//
LOCUS       DAORPW010000302         2227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_18671_length_2227_cov_525.847145, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000302 DAORPW010000000
VERSION     DAORPW010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18671_length_2227_cov_525.847145"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..771
                     /locus_tag="PLB86_13600"
     CDS             163..771
                     /locus_tag="PLB86_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HRC66228.1"
                     /translation="MSEIKLPAERRNSFGKGAARKIRRDHKIPAVLYGHGTDPVHITL
                     PAHDTALALKHANALLSIELEGKSQLALAKDIQRDPIRPIIEHIDLVIVRKGEKVNVD
                     VPVHIEGEAGPDTIVNVESNTIELLVEATHIPESVVVSVEDLPAGTQIKAGELVLPEG
                     ATLVTDEDALIVNITGLSAQEPDADAEAEPAAAATATADAAE"
     gene            783..1436
                     /gene="pth"
                     /locus_tag="PLB86_13605"
     CDS             783..1436
                     /gene="pth"
                     /locus_tag="PLB86_13605"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRC66229.1"
                     /translation="MATWLVVGLGNPGPRYAGNRHNVGAMVVAELARRYAGGSGSTPS
                     SGAPVVTTSLEHLKAHKARAHVAAVRLGTLPGGAPGPAAALAVPTAYMNESGGPVKAL
                     AQFYSTPLEQLVVVHDDLDLTAGTIRLKLGGGEGGHNGLRSITGAMGSRDYLRVRVGI
                     GRPPGRMDPADFVLRDFAPTERAELPFTVSDGADAVELLIQQGLVAAQQRVHAPGPL"
     gene            complement(1443..>2227)
                     /locus_tag="PLB86_13610"
     CDS             complement(1443..>2227)
                     /locus_tag="PLB86_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66230.1"
                     /translation="VSFDETGTSWTGHPLDPTEAVSRLKALDHQGKIVLAPGRFFSLG
                     RNALASETAQRQNALDLASEGAAWVLQLDSDEIVADPATFLSCLEQADREGFDALDYP
                     ARYVYQHIRDRWYLERGRRLWRAAAAYPGAVAVRAGTRLRLSRQTDAALFRVDFSRRN
                     TDPWHPPSAIVHRVVHPGQAIMHLSWVRSESAMLAKTSTWGHAGERDWSADVLQWSRR
                     GRRPLRTVAAGQLSRNPDNWLRFLRLADTPDAAARARNGLTS"
BASE COUNT          345 a          795 c          754 g          333 t
ORIGIN      
        1 cagttgcctc ggcgagggac gtgccatgac ggctcgtccg tgatcgacaa ggtgtgccgt
       61 tcagtcggct cgcctctgcc gcgcgtgtcg cgtcccgcgt cgaggcccca gccctgcggc
      121 atacctccgt ggttgccgct cacgtctgtt gcaaggagcg tcatgtccga aatcaagctt
      181 cccgccgagc gccgcaattc gttcggcaag ggtgccgccc gcaagatccg ccgcgaccac
      241 aagatccccg cggtgctcta cggacacggc accgacccgg tgcacatcac cctcccggcc
      301 cacgacacgg cgctcgcgct gaagcacgcc aacgcactgc tgtcgatcga gcttgagggc
      361 aagagccagt tggccctggc caaggacatc cagcgcgacc cgatccgccc gatcatcgag
      421 cacatcgacc tggtcatcgt ccgcaagggc gagaaggtca atgtcgacgt gcccgtgcac
      481 atcgagggcg aggccgggcc ggacaccatc gtcaacgtcg agtccaacac catcgagttg
      541 ctcgttgagg ccacgcacat ccccgagtcg gttgtcgtgt ccgtcgagga cctgccggcc
      601 ggcacgcaga tcaaggccgg cgagctcgtg ctccccgaag gcgcgaccct cgtcaccgac
      661 gaggacgccc tcatcgtcaa catcaccggc ctgtcggcgc aggagcccga cgcggacgcc
      721 gaggctgagc cggcggctgc cgccaccgcc accgccgacg ccgcggagta acccaccccg
      781 ccatggccac ctggctggtc gtcggcctgg gcaaccccgg gccgcgctac gcagggaacc
      841 gacacaacgt cggcgccatg gtcgtggccg agctggcccg ccggtatgcc ggtgggtccg
      901 gctcgactcc ctcctccggg gccccggtgg tcaccaccag cctcgaacac ctcaaggccc
      961 acaaggccag agcccatgtc gctgcggtgc gcctggggac cctgcccggc ggggcccctg
     1021 gcccggcagc ggcgttggcg gtgcccacgg catacatgaa cgagtccggt gggcccgtga
     1081 aggcgctggc gcagttctac tccacgccgt tggagcagct cgtcgtcgtg cacgacgacc
     1141 tcgacctcac cgcggggacg atccggctca aactcggggg cggcgagggc ggccacaacg
     1201 ggctgcgctc gatcacggga gccatgggat cgcgtgacta cctgcgggtg cgggtcggca
     1261 tcggacggcc tcccggccgc atggacccgg ccgacttcgt cctgcgcgat ttcgctccga
     1321 ccgagcgcgc tgagttgccg ttcacggtga gcgatggggc agatgctgtc gagctgctga
     1381 ttcagcaggg cctggtggct gcacagcagc gggtgcatgc cccgggcccg ctctgaggcg
     1441 actcagctgg tcagcccatt gcgagcccga gctgctgcgt ccggtgtgtc ggccaatcgc
     1501 aggaaccgca gccagttgtc ggggttgcgg gacagctgcc ctgcggcgac ggtgcgcagc
     1561 ggacgacggc cccgcctcga ccattgaagg acgtcggcgc tccaatcccg ttcgccggca
     1621 tgtccccagg tgctcgtctt ggccagcatg gccgattcgg agcgcaccca gctcagatgc
     1681 atgatggcct gcccagggtg gacgacccga tgcacgatcg cgctcggcgg atgccaggga
     1741 tcggtgttcc ggcgcgagaa gtccacgcgg aacagggccg cgtcggtttg gcgggacagg
     1801 cgcaaccggg tccccgcccg cacggcgacg gccccggggt atgccgcggc agcgcgccac
     1861 agccgtcggc cacgttccag gtaccaccgg tcccgtatgt gctggtagac gtagcgggct
     1921 gggtagtcca aggcgtcgaa accctcccgg tcagcctgtt cgaggcacga caagaatgtg
     1981 gcggggtcgg cgacgatctc gtcggagtcc agctgaagga cccaggcggc accctcgctc
     2041 gccaggtcca gggcgttctg ccgctgggct gtttcgctgg cgagggcgtt tcgtcccagg
     2101 ctgaagaacc tgcccggcgc gaggacgatc ttgccctgat ggtccagcgc cttcagacgg
     2161 ctcaccgcct ccgtcgggtc gagggggtgc cccgtccacg acgtccccgt ctcgtcgaaa
     2221 ctgacga
//
LOCUS       DAORPW010000303         2195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_19144_length_2195_cov_776.812617, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000303 DAORPW010000000
VERSION     DAORPW010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19144_length_2195_cov_776.812617"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /gene="rlmB"
                     /locus_tag="PLB86_13615"
     CDS             <1..902
                     /gene="rlmB"
                     /locus_tag="PLB86_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRC66231.1"
                     /translation="SGGRYAARPASGGRPSSGGRPGSSGGRSAGSGRPGNSRPTNSGR
                     RGKPSSEIVAGRNSVVEALRADIPAATLYIASRIDSDDRVREALTLATRRGIPMLECP
                     RAELDRITDGAVHQGLALQVPPYTYADPDDLIDPQTPGVPLVVALDGITDPRNLGAIL
                     RSVAAFGGHGVVVPERRSVGMTASAWKTSAGAAARVPVALAGNLTRALERFQKAGFVV
                     VGLDMDGDVTLPEIDLGDDPVVIVVGSEGKGLSRLVRETCDRVVSIPMAGPVESLNAG
                     IAASVTLYEIARQRANVAARRVR"
     gene            1020..1907
                     /locus_tag="PLB86_13620"
     CDS             1020..1907
                     /locus_tag="PLB86_13620"
                     /inference="COORDINATES: protein motif:HMM:NF017831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF929 family protein"
                     /protein_id="HRC66232.1"
                     /translation="MGKSARAQNARLVELRAQQAKAERRSKLMIAGATVGVVAIVVIA
                     LVIAALQGGTDVTGQSSTALDQATFAKVTSVPAASLDTVGAGASDNPPKTIDAPALTK
                     DGKPRVLYVGAEYCPYCATERWPLVVALSRFGTWNNLSASFSAPAPEVNPNTATVSFH
                     GATYSSQYVSFTGYETSTNKMTNGQWGKLDTLEGEDLALFQKYNAPPYVQSSGAIPWI
                     NLGGTSIQAGASYNSAVILNKTQAEIATALADPTTDIAKGVNGAANVLTAQICKSTNQ
                     QPTAVCTAPGVVAAAAKLQ"
     gene            1904..>2195
                     /locus_tag="PLB86_13625"
     CDS             1904..>2195
                     /locus_tag="PLB86_13625"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HRC66233.1"
                     /translation="MSTSSSRSGASAGGSNQSSGSAKPTKSAGSAARPSRSRDRIVPD
                     WLPKVALAICAIGFLVSIYLTIEAFTTGVTLACPEGETINCAKVTESEWSKLF"
BASE COUNT          347 a          790 c          736 g          322 t
ORIGIN      
        1 gttccggcgg caggtatgcc gcccgccccg cctccggtgg tcggccctcc tcgggtgggc
       61 ggcctggctc ctcaggcggc cgatctgcgg gttcgggacg tccgggcaac agtcggccca
      121 ccaacagcgg tcgccgcggc aagccgagct ccgagatcgt cgccgggcgc aactcggtcg
      181 tcgaggccct gcgtgccgac atcccggccg cgaccctcta catcgccagc cggatcgact
      241 ccgacgaccg agtccgtgag gcgctcaccc tggcgacgcg acgcggcata cccatgctgg
      301 aatgtccccg cgcggaactc gaccggatca ccgacggcgc cgtccaccag ggcctcgcgc
      361 tgcaggtgcc gccctacacg tatgccgacc ccgacgatct catcgacccg cagacccccg
      421 gcgtgcccct ggtcgtggcc ctcgacggca tcaccgaccc gcgcaacctc ggcgccatcc
      481 tgcgctcagt cgcggcgttc ggcggtcacg gggtcgtcgt gcccgagcgc cggtcggtcg
      541 gcatgaccgc gagcgcgtgg aagacctcgg ccggagcagc cgcgcgggtt ccggtcgcgc
      601 tcgcagggaa cctcacccgc gccctggaac gtttccagaa ggcaggtttt gtcgtcgtcg
      661 gtctcgacat ggacggcgac gtcacgctcc ccgagatcga tctcggcgac gacccggtcg
      721 tcatcgtcgt cggatccgag ggcaagggcc tgtcccgcct cgtgcgggag acgtgtgatc
      781 gggtcgtgtc gattcccatg gccggcccgg tcgagtcact caacgccggc atcgcggcga
      841 gcgtcacgct ctatgagatc gcccggcagc gcgccaacgt cgcggcccgc cgcgtccgat
      901 agggccagcc cgactgtccg aaaggacagg tgcacgacgg cttggtgccc gacacctagg
      961 tccgctggtt agcatgtggc caccgctcgt cgcggtgcca ggacagcaag gagtatgccg
     1021 tggggaagag cgcccgggct cagaacgccc gtctcgtgga gttgcgtgcg cagcaggcca
     1081 aggccgagcg tcgcagcaag ctgatgatcg ccggcgcgac cgtcggtgtg gttgccatcg
     1141 tggtcatcgc cttggtcatc gccgccttgc agggagggac ggacgtcacc gggcagtcgt
     1201 cgaccgcctt ggatcaggcg accttcgcca aggtgacgag tgtgccggcc gcgtcgctgg
     1261 acaccgtcgg cgctggggcc agcgacaacc cgcccaagac gatcgacgct cccgccctga
     1321 ccaaggacgg caagccccgc gtcctctacg tcggtgccga gtactgcccc tactgcgcga
     1381 ccgagcggtg gccgctcgtc gtcgccttgt cgcgattcgg cacgtggaac aacctcagcg
     1441 cgtcgttctc ggcgcctgcc cccgaagtga accccaacac cgcgacggtc tcgttccacg
     1501 gggcgacgta ctccagccag tacgtctcgt tcaccggcta tgagacctcg acgaacaaga
     1561 tgaccaacgg ccagtggggc aagctcgaca ccctcgaggg cgaggacctg gcgctgttcc
     1621 agaagtacaa cgccccgccg tatgtccaga gcagcggcgc gatcccgtgg atcaacctgg
     1681 gcgggacctc gatccaggcc ggtgcgtcgt acaacagcgc ggtgatcctc aacaagaccc
     1741 aggccgagat cgctaccgcg ctggctgacc cgacgaccga catcgccaag ggagtcaacg
     1801 gcgcggccaa cgtgctcacc gcccagatct gcaagtcgac caaccagcag ccgacggccg
     1861 tctgcacggc gccgggtgtg gttgcggcgg cagcgaaact ccagtgagca cgtcgtcgtc
     1921 caggtccggg gcgtcggccg gcgggtcaaa ccagtcgagc gggtcggcca agccgaccaa
     1981 gtcggccggg tcggccgctc ggcccagccg gtcccgcgac cggatcgtgc ccgactggct
     2041 gcccaaggtg gcgctggcga tctgtgcgat cggcttcctg gtgtcgatct acctcaccat
     2101 cgaggcgttc accaccgggg tgacgctggc ctgccccgag ggcgagacga tcaactgcgc
     2161 caaggtcacc gagagcgagt ggtcgaagct gttcg
//
LOCUS       DAORPW010000304         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_19496_length_2174_cov_729.916470, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000304 DAORPW010000000
VERSION     DAORPW010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19496_length_2174_cov_729.916470"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1257)
                     /locus_tag="PLB86_13630"
     CDS             complement(40..1257)
                     /locus_tag="PLB86_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRC66234.1"
                     /translation="MGDTGSVWQRWLDFRQRQRAQLTEESESAAEDGAGEGSVIPAPT
                     PAPPTPGSAHPPAGSPLNRSSPFYLGLVGGLGVLIAYGLWQILATLDTVLTLLVVALF
                     LALALNPLVERLVAAGVRRGRAVAIVAGLVVLIVGLIGLVVVPPVVQQGGDLARHAPR
                     YLDNLLADPRLQELDATYHFLDKVRDEINRRLTDGAFMSQAVGGVLGAGMAVVSGVFS
                     TVTVLVLTLYFLASLPTIKHAAYAMVPASRRARVESLAEAMMRRVGSYAIGQVLVATI
                     NACCSYVMMKILGIPYAAVLAVTVGLLGLIPMVGATLGAIVVALVALFVDPTKALIAV
                     VYFVVYQQIENYVVAPRMMRRTVSVPGVVTVVAALAGGTLAGVLGALLAIPTAAGLLL
                     LYEEVLVPRQQRH"
     gene            complement(1402..>2174)
                     /locus_tag="PLB86_13635"
     CDS             complement(1402..>2174)
                     /locus_tag="PLB86_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRC66235.1"
                     /translation="HGWGENAVKEGDVALIWGGAGGLGSMAIQIVKAAGGIPIAVVSG
                     EDKFDYCMKLGAKGCINRNDFDHWGMLPHWKDDAAYGTWLKGARKFGSAIWDVLGERR
                     APNIVFEHPGESTIPSSIFVCDTGGMVVVCAGTTGYNATVDLRYLWMRQKRLQGSHFA
                     NSVQANQMNQLALAGQLDPCLSQAFTYDEIPLAHDLMYRNKHPHGNMAVLVGATDFGQ
                     GITAPEPVALPHPSLPDGDAHTTPRILPHATDSELLPE"
BASE COUNT          370 a          727 c          768 g          309 t
ORIGIN      
        1 tgcggcgagc gtgccaccgg ggccgacgag cggtcgcgat cagtggcgct gctggcgagg
       61 gacgaggacc tcttcgtaga gcagcaagag gccggcggcg gtcgggatcg ccaacagggc
      121 tcccagcacc ccagccaggg tgccgccggc gagggcagcc acgaccgtca ccacaccggg
      181 caccgagacg gttcggcgca tcatccgtgg cgccaccaca tagttctcga tctgctgata
      241 gacgacgaaa tacacgaccg cgatgagcgc cttcgtcggg tccacgaaga gcgcgacgag
      301 cgccaccacg atggcgccca gcgtggcgcc gaccatgggg atgagaccca gcaggccgac
      361 cgtgacagcc agcaccgcgg catacgggat gccgaggatc ttcatcatga cgtaggagca
      421 gcacgcgttg atggtggcca cgagcacctg cccgatggcg taggacccca cccgacgcat
      481 catcgcctcg gcgagtgact ccacccgcgc gcgtcggctg gccgggacca tcgcgtatgc
      541 cgcgtgcttg atcgtcggga gcgacgcgag gaagtagagg gtgaggacca gaacggtgac
      601 cgtcgagaag actcccgaga cgacggccat cccggcgccg agcacgccgc ccacggcctg
      661 ggacatgaag gcgccatcgg tgagccggcg gttgatctcg tcgcggacct tgtccaggaa
      721 gtggtaggtc gcgtcgagct cctgcagtcg cgggtcggcg agcaggttgt cgaggtagcg
      781 gggagcatgg cgtgccagat cgccaccctg ctggacgacc ggcggcacga cgacgagacc
      841 gatcaagccc acgatgagca cgaccagccc cgccacgatg gccaccgccc gtccccggcg
      901 caccccggcg gccacgagac gctcgacgag cgggttgagt gccagggcga ggaagagggc
      961 gacgacgagg agggtgagca cggtgtcgag ggtggccagt atttgccaga gcccgtaggc
     1021 gatcagcacc cccaggcccc cgaccagccc gagatagaag ggggacgacc ggttgagcgg
     1081 actgccagcg ggcggatggg ccgaaccggg tgttggcggc gccggggttg gcgcagggat
     1141 cactgatccc tcacccgccc cgtcctcggc cgcgctctcg gattcctccg tcagttgggc
     1201 gcgctggcgt tgccggaagt ccagccaacg ctgccacacc gagccggtgt cgcccatcac
     1261 ccacctccac acgccgacga cgcatccacg atcgtcgagc ctaagggagc cgtgtcccgc
     1321 aggcgcggcg gcgcggcata cccggtcggt atgccgcgcc gagacgctcg tccgctacgc
     1381 gcctgcgcgc gcagcgccgg gtcactccgg gagcaactcg ctgtcggtcg cgtgggggag
     1441 gatccggggc gtcgtgtggg cgtcaccgtc gggcaggctc gggtgcggaa gagccaccgg
     1501 ctctggcgcg gtgatgccct gaccgaagtc cgtggcgcca acgaggaccg ccatgtttcc
     1561 gtgcgggtgc ttgttgcggt acatgaggtc gtgggccagg gggatctcgt cgtaggtgaa
     1621 ggcctgcgac aggcacgggt cgagctgccc ggccaacgcc aactggttca tctggttggc
     1681 ttgaacggag ttggcgaagt gcgagccttg caggcgcttc tggcgcatcc acaggtagcg
     1741 caggtcgacg gtcgcgttgt agcccgtggt gcccgcgcag acgacgacca tgccgccggt
     1801 gtcgcagacg aagatcgagc tgggaatcgt gctctcgccc gggtgctcga agacgatgtt
     1861 cggtgcgcgc cgctcgccca ggacgtccca gatcgccgaa ccgaacttgc gcgcaccctt
     1921 gagccaggtc ccgtatgccg cgtcgtcctt ccagtggggc agcatccccc agtggtcgaa
     1981 gtcgttgcgg ttgatgcagc ccttggcgcc gagcttcatg cagtagtcga acttgtcctc
     2041 gcccgaaacc acagcaatgg gtatgccgcc ggccgccttg acgatctgaa tcgccatcga
     2101 gccaaggccg cccgcgccgc cccagatgag ggcgacgtca ccttccttga cggcgttctc
     2161 gccccagccg tgca
//
LOCUS       DAORPW010000305         2162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_19681_length_2162_cov_830.453726, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000305 DAORPW010000000
VERSION     DAORPW010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19681_length_2162_cov_830.453726"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLB86_13640"
     CDS             complement(<1..629)
                     /locus_tag="PLB86_13640"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRC66236.1"
                     /translation="MTKELYDLGEQPPLGEIPAKMHAWLIRPERFGPPLEAFQKEVVD
                     VPTIADDEVLVYVMAAGINYNNVWAGLGIPVDVVGARNKAGEPERFHIGGSDASGIVY
                     AVGKDVTNVKVGDEVVMHCGKWDRDCPTVQRGGDPMYSPSFRIWGYETNYGSFAQFTK
                     VQGQQCMPKPKHLTWEAASAYTLVAATAWRMLHGWGENAVKEGDVALIWG"
     gene            complement(825..1262)
                     /gene="mce"
                     /locus_tag="PLB86_13645"
     CDS             complement(825..1262)
                     /gene="mce"
                     /locus_tag="PLB86_13645"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRC66237.1"
                     /translation="MDAPLFTMIDHVGIAVADLDAAIAFYRDTYGMALLHEETNVEQG
                     VREAMMAVGHSGSCIQLLAPLSPESPIGKFLTRSGPGIQQMAYRVEDIDAVCATLRER
                     GVRLLYDVPKRGTSDSRVNFIHPKDAGGVLVELVEPATDTPHH"
     gene            1694..>2162
                     /locus_tag="PLB86_13650"
     CDS             1694..>2162
                     /locus_tag="PLB86_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRC66238.1"
                     /translation="MSSNTSVIVAGARTGMGRLLGSLKDFSATDLGGIAIKGALEKSG
                     ITGDQVDYVIMGHVLQAGCGQVTARQAAVKGGIPMDVPSITINKVCLSGINAIATADQ
                     LIRSGECEIVVAGGMESMTNAPHLLEKSREGFKYGTVKMRDHMDYDALWDVYTD"
BASE COUNT          342 a          720 c          735 g          365 t
ORIGIN      
        1 ccccagatga gggcgacgtc accttccttg acggcgttct cgccccagcc gtgcagcatc
       61 cgccacgctg tcgctgcgac gagggtgtag gccgaggcgg cttcccaggt caggtgtttg
      121 ggcttgggca tgcactgctg gccctggacc ttggtgaact gggcgaagct gccgtagttg
      181 gtttcgtagc cccagatccg gaagctcggc gaatacatcg ggtcgccgcc gcgctggacc
      241 gtggggcagt cgcggtccca cttgccgcag tgcatgacga cctcgtcgcc gaccttgacg
      301 ttggtgacgt ccttgccgac ggcatacacg atgcccgagg cgtcgctgcc gccgatgtgg
      361 aagcgctccg gctcgcctgc cttgttgcgg gcgccgacga cgtcgaccgg gatgccgagg
      421 ccggcccaca cgttgttgta gttgatgccg gctgccatga cgtagacgag cacctcgtcg
      481 tcggcgatcg tcgggacatc gacgacctcc ttctggaagg cctccagcgg cgggccgaag
      541 cgctcagggc ggatcagcca ggcatgcatc ttcgcgggga tctcgcccag gggaggctgc
      601 tcgcccaggt cgtagagttc tttggtcatc gtgggtcctc tctgcaaagg cgggccgccc
      661 tcgggctggg cggccagcgc tggagatcga cgacgcgcca acttttctgg cgctcttcgc
      721 tcgaccgtac tcgcaagccg aaggcctgct attgggcaca acccgtgacg ttgcccacct
      781 gagaagcgtg gcggcgcagc accgggtcgg gtggtgagcg accgtcagtg atggggcgta
      841 tcggtggcgg gctcgacgag ctcgacgagc accccgccgg cgtccttggg gtggatgaag
      901 ttgacccggc tgtcgctcgt cccacgcttg gggacgtcgt agagcagccg cacaccgcgc
      961 tcgcgcaggg tggcgcagac ggcatcgatg tcctcgaccc ggtaggccat ctgctggatc
     1021 cccgggccgg agcgcgtgag gaacttgccg atcggcgact ccggcgacaa gggggcgagt
     1081 agttggatgc acgaccccga gtgcccgacg gccatcatcg cctcgcgcac cccctgctcc
     1141 acgttggtct cctcgtgcag cagggccatg ccgtaggtgt cgcggtagaa ggcgatggcc
     1201 gcatcgaggt cggcgacggc aatgccgacg tggtcgatca tcgtgaagag gggggcgtcc
     1261 atacctctga gcctacggcc gcggcatacc gtcgggcacg ccggaagggc ctcgaaggcg
     1321 gcggtgtgac ggccttcatg ctgccgccga cccgcctgcg gataccgcag ttgaatcact
     1381 ctcagacaac gaaaatcgac agtctcggca agagttccag tcgaatcctc ggaaaactct
     1441 ggcgctcacc cggtctggcc gtctaccgtc cgacccgagc ctgtgccgca ggagggaacg
     1501 ctcaggcccc ggaccctggg gtgggggatt cgcgcggcat accggagggc cgaccgcccg
     1561 ccggtatgcc gcgcggtcgc acccgtgtgt ggtgcgtccc accagagctc cccgggtcca
     1621 ccgtcactcg ccgcggatac agtgatcgat atctgacgtc tctcgctcct gatcgccccc
     1681 tggaggctgc gctatgagct cgaacacctc tgtcatcgtc gctggtgccc gcaccggcat
     1741 gggccggctg ctcggctcgc tcaaggactt ctcggcgacc gacctgggcg gcatcgccat
     1801 caagggcgcc ctggagaagt ccggcatcac gggtgaccag gtcgactacg tcatcatggg
     1861 ccacgtcctg caggccggct gcggccaggt gacggcgcgc caggcggcgg tcaagggtgg
     1921 catccccatg gacgtcccgt cgatcacgat caacaaggtc tgcctgtcgg gcatcaacgc
     1981 catcgccacg gccgaccagc tcatccgctc gggtgagtgc gagatcgtcg tcgctggcgg
     2041 catggagtcg atgaccaacg cgccccacct gctggagaag agccgcgagg gcttcaagta
     2101 cggcacggtc aagatgcgcg accacatgga ctacgacgcc ctgtgggacg tctacaccga
     2161 cc
//
LOCUS       DAORPW010000306         2138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_20047_length_2138_cov_858.219395, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000306 DAORPW010000000
VERSION     DAORPW010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20047_length_2138_cov_858.219395"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1172
                     /locus_tag="PLB86_13655"
                     /pseudo
     CDS             45..1172
                     /locus_tag="PLB86_13655"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
     gene            1668..2132
                     /locus_tag="PLB86_13660"
     CDS             1668..2132
                     /locus_tag="PLB86_13660"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRC66239.1"
                     /translation="MTENASDTATETSAETTKPSVLFVCVHNAGRSQMGAAYTHHLSG
                     GAVEVRSAGSAPAETINPAVREAMLEEGIDISAEKPKILTTEAVQASDVVITMGCGDT
                     CPYFPGKRYLDWVLEDPAGQGVASVRPIRNEIKARVLGLLGELGVAPVELSR"
BASE COUNT          313 a          804 c          721 g          300 t
ORIGIN      
        1 ccgagcaggc cgccgacgcc tatcgcgagc ggatcgtcgg gccggtgcgc gacctgctcc
       61 ccgccgccga gatcgcctcg atcacagagc aactgtccgg ttcctgcacg accgaggtcg
      121 cctcgttcaa cgagttcacg gcgctgttgg ccgatgacac ggtgatcgca ggctatgacc
      181 acgtcatctt cgacaccgcg ccgaccgggc acaccatccg cctgctgcac ctgcccggcg
      241 aatggaccga cttcctcgac gcgggcaagg gtgacgcgtc gtgcctcggc ccgatggccg
      301 gcctggaccg ggctcgcacg acctacgcga ccgccctgca tcggctggcc gacccggcgt
      361 ggacccggat ggtcctcgtc gcccgcgcgc agacctcctc gctcgctgaa gccgcccgca
      421 ccgcaacgga actcgctgag atcggcatct ccgaccagcg cctggtcatc aacgccgtcc
      481 tcccgccgga cccctccggc gaccggctcg ccgccgccgt gcgagtccgc gagtcccgcg
      541 ccttggccgc gctgccggct gccctgctcg gtatgccgcg cgacgaggtc cccctgcgcg
      601 ccgacacggt cgtcggggtc gccgccgtcc gccacctgtt ggacgaccca gcacccgccg
      661 acggcgctgc gggggatgcg cgggatgccg aactccgctc ggacatggcg gtcgctgggg
      721 cgaacggacg cctagcggcc cccaacgaca gcaaggtcga gctgcccggg ctggacaccc
      781 tcgttgacga attggcgcgc caagaccacg gattggtcat gtgcatgggc aagggcggcg
      841 tgggcaagac caccgtcgcg gccgccatcg ccgtcgcgct ggccgaccgc ggtcacaccg
      901 tccacctgac gaccaccgac ccggccgggc atctcgacga ggcgttgggc ggcgactccg
      961 ccggcgtcgg tgcggggatc ggtgccggta tcaccgtgtc gcggatcgac ccgcgagcgg
     1021 cgacggcggc ataccgggag aaggtcctgg ccaccaaggg agccgcgctg gacggggccg
     1081 ggagggcgcg gctcgccgag gacctgctct cgccgtgcac cgaggaggtg gcggtcttcg
     1141 ggcagttctc gcacctggtg catcaagcgc ggcgccagtt cgtcgtgatg gacacggcac
     1201 cgaccggcca cacgctgctg ttgatggatg cgacgggttc ctaccaccgc gacgtcgtcc
     1261 gcaacctcgg ggcaggcggc gggcaggcaa gcggcgaggg ggccgcgagg ggagccggcg
     1321 gggcggcata cacgacgccg ttgatgcgcc tgcgcgaccc ggacctgacc cacgtcgtca
     1381 tcgtcaccct tcctgacacc acccccgtgc tcgaggcgga ggggttgcag gcggacctga
     1441 tccgcgccga catccacccc tgggcgtggg tcgtcaacgc gtcgctggcc gccgcccacc
     1501 cgaccgctcc cctgctcgct cggcgcgctg ctgccgaggt gccccacctg gcccgggtgc
     1561 gcgaggttgc ccctcgcgtc gcggtcatcc ccctgctcgc cgatgaaccc atcggtgcag
     1621 aacgtctgag atcccttgtc cgccaacccg atccgaagga aacttccatg accgagaatg
     1681 cttccgacac cgccactgag acctctgccg agacgaccaa gccgtccgtg ctcttcgtct
     1741 gcgtccacaa tgccggccgc tcgcagatgg gcgcggccta cacccaccac ctgtccggtg
     1801 gggctgtcga ggtgcgctca gcaggctcgg cacccgccga gacgatcaac ccggcagtgc
     1861 gcgaggccat gctcgaggag ggcatcgaca tctccgccga gaagcccaag atcctcacga
     1921 ccgaggcggt ccaggcctcc gatgtcgtca tcaccatggg ctgcggcgac acgtgcccct
     1981 acttcccggg caagcgttac ctcgactggg tgctggagga cccggccggc caaggggtcg
     2041 cgtccgtccg accgatccgc aacgagatca aggcgcgggt cctcggcctg ctcggcgagc
     2101 tcggcgtcgc gccggtggag ttgtctcgat gaccgacg
//
LOCUS       DAORPW010000307         2126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_20228_length_2126_cov_574.300821, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000307 DAORPW010000000
VERSION     DAORPW010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20228_length_2126_cov_574.300821"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLB86_13665"
     CDS             <1..379
                     /locus_tag="PLB86_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC66240.1"
                     /translation="EPRKGFWTLPAGFLEYGETLADGAARETREEAGAWIEIDGLFTV
                     LDVVQAGQVHVFYRARMLRPDLDPGPETIKARLVPLDEIPWDDLAFETVRWTLKRYVE
                     DRSAGNFGIHAASIGRRPRESRS"
     gene            656..1669
                     /locus_tag="PLB86_13670"
     CDS             656..1669
                     /locus_tag="PLB86_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRC66241.1"
                     /translation="MATISTYPFVTHLRSTATMHVLHRSRGRLRHTGPGAAFWFRPMS
                     AAISEVPVDDREQPVLITVRTADLQQVSAPGTVTYRFVDPALAATHVDFSVQLKTGAW
                     AETPLETVGSAIHGATAAAVTQALVGSDLRTALTTDPAWLAATVRAALAADERLTSTG
                     LAVVGVRFAVLRAEPDVERALQTPAREAIQQEADKATFERRAVAVEREAAIGQNELAN
                     QIDLAARAEQLIAQKGTNARREAQEKAAAGAIATAAEAARTTQLAQAQAEADQVVGAA
                     AAETERAKLAAYTGMPRDLLLALAAREAAASLPQIDQLVITPDLLSSLLGRLAGGTTA
                     QEG"
     gene            1673..>2126
                     /locus_tag="PLB86_13675"
     CDS             1673..>2126
                     /locus_tag="PLB86_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66242.1"
                     /translation="MGTPRAVLVRRTTELDELVAHHGTRGQAEFFLRTREQSIEPLVQ
                     RHQGTQAARQTVLAAVPADWRRAEVERAELARFVFEPDDVVIVVGQDGLVANVAKYLG
                     AQPVIGIDPLPGANPGVLVPHPAAATGDLVRDVVAGRAAYLERTMVRAV"
BASE COUNT          331 a          787 c          717 g          291 t
ORIGIN      
        1 cgagccgcgg aagggcttct ggaccttgcc tgctgggttc ctcgaatacg gcgagacgct
       61 cgcggacggc gccgcccgcg aaacccgcga ggaggccggc gcctggatcg agatcgatgg
      121 gctcttcacc gtgctggatg tcgtccaggc cggtcaggtc cacgtgttct accgggctcg
      181 aatgctgcgc cccgacctcg acccaggtcc cgagacgatc aaggcgcggc ttgtgcctct
      241 cgacgagatc ccctgggacg acctggcctt cgagacagtg cgctggaccc tcaagcgcta
      301 tgtcgaggac cgatccgccg ggaacttcgg gatccacgcc gcatccatcg gacgacggcc
      361 gagagagtca aggagctagg tcatgcgcat gagccgggcg atagtggcgg ccgcggtgct
      421 ggccgcagtg gcgtggtcgt tctggggctg gtcatggcaa gcggtggtcc tgggtgcgta
      481 ccttccaact ctcctgcttg gtctggggat cgagtacctc gttgcgcgtc ggcgccgctt
      541 cggcaacgac accgcgtccc ccggaccgtc aaaaaagtca gattgacaat aacgccagaa
      601 ggcctataga gtcagagtga cgaaaagccg tcaaccctga tccaaggaga gcctcatggc
      661 caccatcagc acctacccgt tcgtcaccca cctgcgcagc acggcgacca tgcacgtcct
      721 gcaccgctcg cgcggccggc tccggcacac cggccccggc gccgcgttct ggttccggcc
      781 gatgtcggcg gcgatcagcg aagtccccgt cgacgaccgc gagcagccgg tgctcatcac
      841 tgtgcgcacc gccgacctgc agcaggtcag cgcccccggc acggtcacct accgcttcgt
      901 cgaccccgcc ctcgcggcga cccacgtcga cttctcggtc cagctcaaga ccggcgcgtg
      961 ggccgaaacc ccgctcgaga cagtcggatc cgcgatccac ggcgccacgg cagcagcggt
     1021 cacccaggcc ctggtgggta gcgacctgcg gaccgccttg accaccgacc cggcctggtt
     1081 ggccgccacc gtccgggccg ccctcgcagc cgacgagcgg ctgacctcca ccggtctggc
     1141 ggtcgtcggg gtccgtttcg ccgtgctgcg cgccgagccc gacgtcgagc gcgccctgca
     1201 gacccccgcc cgcgaggcga tccagcagga ggccgacaag gccaccttcg agcgccgggc
     1261 cgtcgccgtc gagcgcgaag cggctatcgg tcagaacgag ttggccaacc agatcgacct
     1321 ggccgcccgc gccgaacaac tcatcgcgca gaagggcacc aacgcccggc gcgaagccca
     1381 ggagaaggcg gcggccggcg ccatcgccac ggctgccgag gccgctcgca cgacccaact
     1441 cgcccaggcc caggccgagg ccgaccaggt ggtcggagcg gcggcggccg agaccgagcg
     1501 ggcgaagttg gcggcataca ccggtatgcc gcgcgacctc ctgctcgcgc tcgctgcgcg
     1561 cgaagcggcc gcgtcgcttc ctcagatcga ccaactcgtc atcacccccg acctgctgtc
     1621 ctcgctgctg ggtcggctgg ccggcgggac caccgcccag gagggctgac ccatggggac
     1681 gccccgagcc gtgctcgtcc ggcggaccac cgagttggac gagttggtgg cccaccacgg
     1741 gacgcgtggc caggccgagt tcttcctgcg cacccgtgag cagtcgatcg agccgctcgt
     1801 ccaacggcat caggggaccc aggccgcccg ccagacggtc ctcgctgcgg tgcccgcgga
     1861 ctggcgccgc gccgaggtcg agcgagccga gttggcgagg ttcgtcttcg agcccgacga
     1921 cgtcgtcatc gtcgtcgggc aggacggcct ggtcgccaac gtggcgaaat acctgggcgc
     1981 gcagccggtg atcggcatcg accccctccc cggcgccaac cccggagtgc tcgtccccca
     2041 ccccgcggcg gccacaggcg acctggtgcg cgacgtcgtc gcggggcggg cggcatacct
     2101 cgagcggacc atggtgcgcg cggtgg
//
LOCUS       DAORPW010000308         2068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_21183_length_2068_cov_662.767511, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000308 DAORPW010000000
VERSION     DAORPW010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21183_length_2068_cov_662.767511"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLB86_13680"
     CDS             complement(<1..371)
                     /locus_tag="PLB86_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HRC66243.1"
                     /translation="MPFAERISRLGTETAFAVSLAAAAWGAQGHRIYPFHLGDIDLPT
                     PANIVEAMDRAIADGKTGYCPGAGIMPLREALAADVAGRRGLDLSADNVTIHPGGKPV
                     ITKFVQALIEFGDEVLYPNPG"
     gene            complement(440..1390)
                     /locus_tag="PLB86_13685"
     CDS             complement(440..1390)
                     /locus_tag="PLB86_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010635806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRC66244.1"
                     /translation="MRIVVALGGNALLRRGEPPTAENQRTNVHAAALALAPLARDHEL
                     VITHGNGPQVGLLALQAAALPGVAAYPLDLLGAQTDGMIGYLIEQELGNLLPENQPLA
                     TLLTMTEVDPDDPAFADPTKFVGSIYDEATARAAAAEHGWTVKPDGPHWRRVVASPKP
                     LRIFEIRPVDLLLRAGCVVICAGGGGIPTMFERDAQGRRSNVLAGVEAVVDKDLASGV
                     LARELGADAFVMATDVPAVIDHWGMPSARQIAAARPEWLLAQGFPAGSMGPKVEAAAA
                     FATETGRPAYIGALEVIGDLLEGRAGTLVSTSLAEPTRLR"
     gene            1509..>2068
                     /locus_tag="PLB86_13690"
     CDS             1509..>2068
                     /locus_tag="PLB86_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRC66245.1"
                     /translation="MAAAPGHIVIACDKFKGSLTAAQVVEAIRDGIASAAPGREVRAV
                     LVADGGDGTLAAAESVGFRHVPVTVAGPTGSPNVSGYAVRAGTAVVELADACGIVHLP
                     GGVKAPLTATSRGLGQVLAAALDDPSVTSVVMGIGGSASNDGGAGMLAALGARLLDAG
                     GRELPDGGGALVDLAALDLSGLHPRLR"
BASE COUNT          265 a          672 c          803 g          328 t
ORIGIN      
        1 tagcccgggt tgggatagag cacctcgtcg ccgaactcga tgagcgcctg gacgaacttg
       61 gtgatgaccg gcttgccgcc cgggtggatc gtgacgttgt cggcgctgag gtccagcccg
      121 cgccgcccgg cgacgtccgc ggcgagcgcc tcgcgcagcg gcatgatgcc ggcgccgggg
      181 cagtagcccg tcttgccgtc ggcgatcgcc cggtccatcg cctcgacgat gttggcgggc
      241 gtcggcagat cgatgtcgcc gagatggaag gggtagatgc ggtgaccctg tgctccccag
      301 gccgcggctg ccagcgagac ggcgaaggcg gtctcggtcc cgagtcgact gatgcgttct
      361 gcgaatggca cttcggtgtg tcccttcgtg tggcggagcg aggacccggg gtgccgggtc
      421 gcccgtgctg tcatctactt catcggagcc gggtgggctc cgcaagactc gtgctgacga
      481 gggtcccggc tcgaccctca agcaggtcgc cgatcacctc gagcgccccg atgtatgccg
      541 gtcggccggt ttcggtggcg aaggccgccg ccgcctcgac cttcggcccc attgagccgg
      601 ccggaaaccc ttgggcgagt agccactccg ggcgagcggc ggcgatctgg cgggcggacg
      661 gcatacccca gtggtcgatg acggcgggga cgtccgtggc catgacgaag gcgtccgcgc
      721 ccaactcgcg ggcgagcacg cccgaggcga ggtccttgtc gacaacggcc tccacgcccg
      781 caaggacgtt ggaccggcgc ccctgcgcat cgcgctcgaa catcgtcggt atgccgcccc
      841 cgccggcgca gatgaccacg caccccgcgc gcaggagcag gtccacaggt cgaatctcga
      901 agatccgcag gggttttggc gaggcgacga cccggcgcca gtgtggccca tcgggtttca
      961 cggtccagcc atgctcggca gcggccgcgc gggcggtcgc ctcgtcatag atcgagccga
     1021 cgaacttcgt gggatcggcg aaggccgggt cgtcggggtc gacctcggtc atcgtcaaca
     1081 gcgttgccag gggctggttt tcggggagga ggttgcccag ctcctgctcg atgaggtagc
     1141 cgatcatccc gtcggtctgc gcgcccagca ggtcgagggg gtaggccgcg acgcccggga
     1201 gcgccgctgc ctgcagcgcg agcagcccga cctgggggcc gttgccgtgg gtgatgacga
     1261 gctcgtgatc gcgggccagg ggagcgaggg caagggcggc ggcgtggacg ttcgtgcgct
     1321 ggttctcggc cgtcggaggc tcgccccgac gcagcagggc attgccgccg agggcgacga
     1381 cgatccgcat tccgggggcc tccttgtccc tgcctgcgag ggtaactctg cggcggcata
     1441 ccgcgaggga ctctcgtccc aatcggtgag ggggtttgcg ccgggcgggc gctggctagt
     1501 gtgtgggtat ggccgcagcc ccggggcaca tcgtcattgc gtgcgacaag ttcaaggggt
     1561 cgctcacggc ggcccaggtc gtcgaggcga tccgcgacgg gatcgcgtcg gccgctcccg
     1621 gccgcgaggt gcgcgcggtg ctcgtcgccg atggtggcga tggcacgctc gccgcggccg
     1681 agtcggtggg cttccggcac gtgccggtga ctgtcgccgg ccccacgggg tcacccaacg
     1741 tcagcgggta tgccgtgcgc gccggcaccg cggtcgtcga gctggccgac gcgtgcggga
     1801 ttgtgcacct ccccggtggg gtcaaggctc cgctgaccgc gacgtcgcgg ggcttgggac
     1861 aggtcttggc ggcggcgctc gatgacccgt cggtgacgtc ggtggtcatg gggatcggcg
     1921 ggagcgccag caacgacggc ggcgcgggaa tgcttgcggc actaggggct cggctgcttg
     1981 atgctggcgg tcgtgagctg ccggacggtg gtggggcgct ggtcgatctg gctgcgctgg
     2041 acctgtcggg gctgcacccg cggctgcg
//
LOCUS       DAORPW010000309         2033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_21810_length_2033_cov_735.600101, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000309 DAORPW010000000
VERSION     DAORPW010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21810_length_2033_cov_735.600101"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLB86_13695"
     CDS             <1..348
                     /locus_tag="PLB86_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HRC66246.1"
                     /translation="WALDHDPAGFRWLNADNAAANTFSYLRFGDKDLGTTGPVLAAVV
                     NFSGQTHEPYRVGVPRGGRWKVVLDTAGFFPDAPSSAGVVIDADDEGWDGQPYAVNVT
                     VPRLSTVWLIPED"
     gene            353..2017
                     /gene="pgm"
                     /locus_tag="PLB86_13700"
     CDS             353..2017
                     /gene="pgm"
                     /locus_tag="PLB86_13700"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HRC66247.1"
                     /translation="MTHERAGTVATSADLVDVAHLVTAYYTGVPDPDNIDQQVAFGTS
                     GHRGSSLRTAFNEAHILATTQAIVDYRRSQGFDGPLFLGRDTHGLSEPAWASALEVLA
                     ANDVTVLVDDHDGYTPTPGVSHAILRANRGKIGGVDDLPSGIGLADGIVVTPSHNPPY
                     DGGFKYNPPHGGPADSDATKVIAAAANAYIRAGLAGVQRIPFARARAAATAYDFLGTY
                     VEDLPNVVDIDAIKAAGIRIGADPLGGASVEFWGAMAERLGIDLTVINPLVDPTWRFM
                     TLDWDGKIRMDCSSPYAMASLIANKDAFAISTGNDADSDRHGIVTPDGGLMNPNHYLA
                     VAIQYLYGGGRPGWRPDGFIGKTLVSSSMIDRVASDLGRRLVEVPVGFKWFVPGLLDG
                     SGPFGGEESAGASFARFDGSVWTTDKDGILLALLASEILARTGSSPSEHYRELTAKHG
                     APAYARIDAPANREQKAKLGALSPADVAADSLAGEPITAKLTEAPGNGAKIGGLKVVT
                     ESAWFAARPSGTEDVYKIYAESFRGAEHLAQVQAEARDVVSAALGG"
BASE COUNT          304 a          722 c          695 g          312 t
ORIGIN      
        1 tgggcgctgg accacgaccc ggccggcttc cggtggctca atgccgacaa cgccgcggcc
       61 aacacgttct cctacctgcg tttcggcgac aaggacctcg gcacgaccgg gccggtgctc
      121 gccgcggtcg tcaacttcag cggccagacc cacgagccct atcgggtcgg tgtcccccgc
      181 gggggccggt ggaaggtcgt cctcgacacg gccggcttct tccccgatgc cccctcgtcg
      241 gcaggcgtcg tcatcgacgc cgacgacgag ggctgggacg gtcagccgta tgccgtcaat
      301 gtcaccgtcc cgcgcctgtc caccgtctgg ctgatcccgg aagactgacc tcatgactca
      361 cgaacgcgcc ggcaccgttg ctacgtccgc cgacctggtg gacgtcgctc acctggtcac
      421 cgcctactac acgggcgtcc ccgacccgga caacatcgac cagcaggtcg ccttcgggac
      481 ctcggggcac cgcggctcga gcctgcggac cgccttcaac gaggcccaca tcctggcgac
      541 gacccaggcg atcgtcgact accggcgctc gcagggtttc gacgggccgc tgttcctcgg
      601 tcgcgacacc cacgggctgt ccgagccggc gtgggcctcg gcgctggagg tcctggccgc
      661 caacgacgtc acggtcctcg tcgacgacca cgacggctac accccgacgc ccggggtttc
      721 gcacgcgatc ctgcgcgcca accgcggcaa gatcggtggg gtcgacgacc tgccgtccgg
      781 gatcggtctc gcggacggga tcgtcgtcac cccgtcgcac aacccgccct atgacggtgg
      841 gttcaagtac aacccgccgc acggcggccc ggccgacagc gacgcgacga aggtcatcgc
      901 ggcggccgcc aacgcctata tccgggcggg gttggccggg gtccagcgga tcccctttgc
      961 gcgggcgcgg gctgcggcga cggcatacga cttcctcggg acctatgtcg aggacctgcc
     1021 gaacgtcgtg gacatcgacg cgatcaaggc cgccgggatc cggatcggcg ccgacccgct
     1081 cgggggtgcg tcggtggagt tctggggcgc gatggccgag cgtttgggga tcgacctcac
     1141 ggtcatcaac ccgctcgtcg acccgacttg gcggttcatg acgctcgact gggacggcaa
     1201 gatccggatg gactgctcct cgccttatgc gatggcttcg ctcatcgcca acaaggacgc
     1261 cttcgcgatc tcgaccggca acgacgccga ctccgaccgg cacggcatcg tcacgcctga
     1321 tggtgggctg atgaacccca accactatct ggccgtggcg atccaatacc tgtatggcgg
     1381 agggcggccg ggttggcggc cggacgggtt catcggcaag acgctggtgt cctcgtcgat
     1441 gattgaccgg gtggcctccg acctcgggcg gcggctggtc gaggtgccgg tgggtttcaa
     1501 gtggttcgtg cctggcctgc tcgacgggtc ggggccgttc ggcggcgagg agtcggcggg
     1561 ggcgtcgttc gcccgcttcg acggctcggt gtggacgacc gacaaggacg ggatcctgct
     1621 ggccctgctc gcctcggaga tcctcgcccg cacggggtcc agcccgagcg agcactaccg
     1681 cgagctgacc gcgaaacatg gcgcaccggc atacgcgcgg atcgacgccc cggccaaccg
     1741 cgagcagaag gcgaagctgg gagccttgtc tcctgccgat gtggcggcgg attcccttgc
     1801 gggagagccg atcacggcca agctcaccga ggctccgggc aacggcgcca agatcggcgg
     1861 gctcaaggtc gtcaccgagt cggcgtggtt cgcggcccgg ccgtcgggca ccgaggacgt
     1921 ctacaagatc tacgccgagt ccttccgcgg cgccgagcac ctcgcacagg tgcaggctga
     1981 ggcgcgcgac gtcgtcagcg cagccctcgg cgggtgacca cagccttctc cac
//
LOCUS       DAORPW010000310         2022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_22002_length_2022_cov_839.042196, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000310 DAORPW010000000
VERSION     DAORPW010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22002_length_2022_cov_839.042196"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2022
                     /locus_tag="PLB86_13705"
     CDS             <1..>2022
                     /locus_tag="PLB86_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66248.1"
                     /translation="SIVVTEQSSERAWFAREADELITPPPSPDEVDLSAHIEPDQVVA
                     QVLGAKADTAWIGHTPCLDRAELVAQLEAAGVRVVGPTAQTIRDLADPARLAALGRES
                     GLSPVPDHLTPADWRRIEIDVLADSAGTVWTLGLRDASIRRGATPVLAEMPAPGVSDT
                     TAGAMSHAVRRLVRAAAYRGAGVVELALAHDGESFWLVGVDTLARTEHALVEEITGAS
                     FIGLRLRLAAGDALPATPPTPNGHAVEVRLLAHDPGREYAASGGALEMLSLPVGTGVR
                     VDSSIREGDLVDGRVEPLIATVTAWGRDREEALDRAHGALERTTVVLEIGMTNRTADL
                     AVLERVAAAEQPFDAGWYDRTLAAGGFTSVADPLAVVAAAVEAYESDSTMVNAAFFAS
                     AARGRPERPERVGSLVQLSYRGHDYRLRVDRTGPRSYRVRGNATVDLIVDRLGDFERR
                     IICNGHKHRLVAVEQGADFRLDLDGVGHTVSREDGVVVRTGWPALVSSLLVEPGQDVS
                     AGQPVAVLESMKMVTTVTAPFDGTVTSLAVSNNTQIERGAPLMRIKAASHQHLDLGPG
                     DGVGAEPESAVDFSALVTSDGNPEGKDATWVHAHLGDYLLGYDLDPESFKGLLKRQAT
                     LGATLPANDPELVGAENALLDLFSDLGALYRPRTEAENPDQMADDNTQE"
BASE COUNT          281 a          781 c          682 g          278 t
ORIGIN      
        1 cgtccatcgt cgtcaccgag cagagcagcg aacgtgcctg gttcgcccgc gaggccgacg
       61 agctcatcac cccgccgccg tcaccggacg aggtggacct cagcgcccac attgagcccg
      121 accaggtcgt cgcgcaggtc ctcggggcga aggctgacac cgcctggatc ggccacacgc
      181 cgtgcctgga ccgggccgag ttggtcgctc aactcgaagc cgccggagtg cgggtcgtcg
      241 gccccaccgc gcagaccatt cgcgacctcg ccgaccccgc ccggctcgcc gccctcgggc
      301 gcgagtcggg gctctcgccc gtccccgacc acctgacgcc cgccgactgg cgtcgcatcg
      361 agatcgacgt gctggccgac tccgccggaa cggtgtggac gctcggcctt cgcgacgcga
      421 gcatccgccg cggcgccacc ccggtgcttg ccgagatgcc cgcccccggc gtgtcagaca
      481 cgaccgccgg cgcgatgtcc cacgccgtgc gccggttggt gcgggccgcg gcataccgtg
      541 gcgcgggcgt tgtcgagttg gccctcgccc acgacggcga gtccttctgg ctggtcggcg
      601 tcgacacgct ggcgcgcacc gagcacgccc tcgtcgagga gatcaccggg gcgagcttca
      661 tcgggttgcg gctgcgactt gctgcgggcg acgcgctgcc cgccacgccg cccacgccca
      721 acggacacgc cgtcgaggtg cgtctgctcg cgcacgaccc gggccgggag tatgccgcgt
      781 ccggcggagc gctggagatg ctctcgctgc cggtggggac cggcgtgcgc gtcgactcga
      841 gcatccgcga gggtgacctc gtcgacggcc gggtcgagcc gctcatcgcg accgtcaccg
      901 cctggggccg cgaccgtgaa gaagccctcg accgcgccca cggcgccctc gaacgcacca
      961 cggtcgtcct tgagatcggc atgaccaacc ggacggctga cctcgccgtt ctggagcgtg
     1021 tcgccgcagc cgagcagccc ttcgatgccg ggtggtacga ccggactctc gcggccggcg
     1081 gcttcacctc cgtcgccgac ccgctggccg tcgtcgctgc ggccgtcgag gcctacgagt
     1141 ccgacagcac catggtcaac gccgccttct tcgcgagcgc ggctcgcggg cgccccgaac
     1201 gccccgagcg ggtcgggtcg ctggtgcaac tgagctaccg cgggcacgac tatcggctgc
     1261 gggtcgaccg caccggtccg cgctcctacc gggtgcgcgg caacgcgacc gtcgacctca
     1321 tcgtcgaccg gttgggtgac ttcgagcgcc ggatcatctg caacggccac aagcaccgcc
     1381 tcgtcgccgt cgagcagggt gccgacttcc gcctcgactt ggacggcgtg ggccacaccg
     1441 tcagccgcga agacggcgtc gtcgtgcgca ccggctggcc ggccctcgtc tcgtcgttgc
     1501 tcgtcgagcc cggccaggac gtctcggccg gtcagcccgt ggctgtcctc gagtcgatga
     1561 agatggtgac cacggtgacc gcgcccttcg acggcacggt gacctccttg gcggtctcca
     1621 acaacaccca gatcgagcgc ggcgcgccgc tcatgcggat caaggccgcc agccaccagc
     1681 acctggacct cgggccaggc gacggcgtcg gcgctgagcc cgagtcggcc gtcgacttct
     1741 cggccctcgt cacctccgac ggcaacccgg aggggaagga cgcgacctgg gtccacgccc
     1801 acctcgggga ctacctgctg ggctatgacc tcgaccccga gagcttcaag gggctgctca
     1861 agcggcaggc caccctcggg gccaccctgc ccgccaacga ccctgagctc gtcggtgccg
     1921 agaacgccct gctcgacctg ttcagcgacc tcggggccct ctaccggccg cgcaccgagg
     1981 ccgagaaccc cgaccagatg gccgatgaca acacgcagga gt
//
LOCUS       DAORPW010000311         2016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_22101_length_2016_cov_733.173381, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000311 DAORPW010000000
VERSION     DAORPW010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22101_length_2016_cov_733.173381"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLB86_13710"
     CDS             <1..587
                     /locus_tag="PLB86_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HRC66249.1"
                     /translation="SAPLLDALLALMLLQHSRREARVDDAGRLVLLPDQDRGRWKRGE
                     IADALGLLAGLPDDPAVLGRRAAEVRLQALIAAEHAVALRAGDTDWRRIANRYAALDR
                     LTASPVVRLNRAVAVAEADGPEAGLALLDGLDEVMPTSHRVSTVRAELLVRAGRAEEA
                     AAAYERAIGLCTNEAERAHLLGRREALGRDPGRP"
     gene            complement(584..1222)
                     /locus_tag="PLB86_13715"
     CDS             complement(584..1222)
                     /locus_tag="PLB86_13715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HRC66250.1"
                     /translation="MNQTSSPSAPAVVDPRLTTDPRPDFFAAADQAVHLIDGLAPADL
                     DRPTPCDDYDVRTLVAHLLTVFRRITHVATGGGWTEVPQVITGVPDESLAEVAAADRD
                     RLVQVWSDDAVLERILSLPPGVQVPGRIGAFRYTQELVTHAWDLAAAVGRVDQLDPAL
                     AEPVIIGAQQFVPRDAREGFPFGAVVDVADDAGPYERLVGWLGRDPNWTPSA"
     gene            1284..>2016
                     /locus_tag="PLB86_13720"
     CDS             1284..>2016
                     /locus_tag="PLB86_13720"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRC66251.1"
                     /translation="MRSDRLLSLLLILQARGSAVAPEVARELEVSVRTVYRDVEALSA
                     AGVPVWTEQGRGGGIRLMPGYRTDMTGLTAAESRALVAMSGRAVPDDLGMGSALASAV
                     HKLVAAVPPSHRSAAEAARARVLVDHAGWYRRTPSAPALEVVQEAVWAQRRLLLDYRH
                     GDGTEESVTVDPYGLVAKTGVWYLVGSTGTGEDHHERMWRVDRVNTAVALDEPATEPD
                     GLDLAAVWERLRQGIERPRGLVPVEV"
BASE COUNT          280 a          681 c          767 g          288 t
ORIGIN      
        1 aatccgcgcc gctgctcgat gcgctgctgg cgttgatgct gctgcagcac tcgcgtcgcg
       61 aggctcgggt ggacgatgcc ggacggctgg tgttgctgcc cgaccaggac cgcggccgat
      121 ggaagcgcgg cgagatcgcc gatgctctcg gtctgctcgc ggggttgccc gacgacccag
      181 cggtgctggg tcgtcgggca gccgaggttc ggctgcaggc cctcatcgcg gccgagcacg
      241 cggtcgcgct cagggccggc gacaccgatt ggcgacggat cgccaaccgg tatgccgccc
      301 tcgaccgcct cacggcctcg ccggtggtcc ggctcaaccg tgcggtggcc gtggctgaag
      361 ccgacggccc ggaggctggc ctggccttgc ttgacggcct cgacgaggtg atgcccacgt
      421 cgcatcgagt gtcgacggtg cgggccgagt tgctggtccg ggccgggcgc gcggaggagg
      481 ccgcggcggc atacgaacgg gcaattggct tgtgcaccaa cgaagccgag cgggcccacc
      541 tgctgggccg ccgcgaggcg ctcgggcgcg acccgggccg accctaggcg gacggcgtcc
      601 agttcgggtc gcggcccagc cagccgacca gccgctcgta aggaccggcg tcgtcggcca
      661 cgtcgaccac cgccccgaag gggaagccct cacgcgcatc gcgcgggacg aactgctggg
      721 cgccgatgat gaccggctcg gcgagtgccg ggtcgagctg atccacgcga ccgacggccg
      781 cagcgaggtc ccacgcgtgg gtgacgagct cctgggtgta gcgaaaggct ccgatgcgtc
      841 ccggcacctg aacgcccggg gggaggctga ggatccgctc taggaccgcg tcgtcggacc
      901 agacctgcac gagccggtcg cggtcggcgg cggcgacctc ggcgagcgac tcgtcgggga
      961 cgccggtgat gacttgtggc acctcggtcc agccgccgcc ggtggccacg tgggtgatcc
     1021 gacggaagac ggtcaagagg tgggcgacga gggtgcgcac gtcgtagtcg tcgcaggggg
     1081 tcggtcggtc gaggtcggcc ggcgcgaggc cgtcgatgag gtggaccgcc tggtcggctg
     1141 cggcgaagaa gtcgggacgg gggtcggtcg tcagacgcgg gtcgacgacg gcgggggcgc
     1201 tgggggaaga agtttggttc atggctccac cctgagccct gaacctgaca cctacggtca
     1261 ggtttctctg ccattctcga aaggtgcgat ccgaccgact cctcagcctt ctcctcatcc
     1321 tccaggcccg cggcagcgcc gtcgcgcccg aggtcgcacg cgagttggag gtcagcgtcc
     1381 gcaccgtcta tcgcgacgtc gaggcactct cggcggccgg cgttccggtg tggaccgagc
     1441 agggtcgagg cgggggcatc cggctcatgc ccggctatcg caccgacatg acggggctga
     1501 ccgcggccga gtcacgggcc ctggtggcga tgtccggtcg agccgtgccc gacgacctcg
     1561 gcatggggtc ggccctggcg agcgcggtcc acaaactcgt tgccgcagtg ccgccttcgc
     1621 atcgctcggc tgccgaagcc gcccgcgcga gggtcctcgt tgaccatgct ggctggtatc
     1681 gccgcacccc ttccgcaccc gcgctcgagg tcgttcagga ggccgtgtgg gcgcagcgcc
     1741 gactgctcct ggactatcgg cacggcgacg gcaccgagga gtcggtcacc gtcgacccct
     1801 atggcctggt cgccaagacg ggagtctggt acctcgtcgg cagcaccggc accggcgagg
     1861 accaccacga acggatgtgg cgcgtcgacc gggtcaacac cgcagtcgcc ctggacgagc
     1921 cggccacgga gccggacggg ctcgacctcg cggcggtgtg ggaacggcta cgacagggga
     1981 tcgaacggcc ccgcgggttg gtgccggtcg aggtcg
//
LOCUS       DAORPW010000312         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_23228_length_1957_cov_578.166141, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000312 DAORPW010000000
VERSION     DAORPW010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23228_length_1957_cov_578.166141"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLB86_13725"
     CDS             complement(<1..292)
                     /locus_tag="PLB86_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRC66252.1"
                     /translation="MGPEAMRVAGIQQTLAQFGVSVNDLGNLAGPPNPWLPPVDGYRH
                     LPEVQAWNQAVHDAVYGSLSGGRLPILLGGDHCLAIGSISAVARHCRDQGLTL"
     gene            complement(379..1278)
                     /locus_tag="PLB86_13730"
     CDS             complement(379..1278)
                     /locus_tag="PLB86_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRC66253.1"
                     /translation="MSAADLPAAEVPKVAEATDGIEWITDPTDPRLADYVALTDVALR
                     RRTEPERGLYVAESEKVIRRALAAGHRPRSFLMAERWLTDLADVVADAREGGIPIYVG
                     EHDVIEALTGFHLHRGALAAMQRPVLPAVGDLLKNARRVLVLEDIVDHTNVGACFRSA
                     AALGVDAVLVTPRCADPLYRRAVRVSMGTVFQVPWTRIEPWPLGGPELLRENGFTVAA
                     FALTETAMTLDELSADPPERLALVLGTEGDGLTRHTIAAADLAVRIPMAGGVDSLNVA
                     AACAVALWALRPSAIGASGAATV"
     gene            complement(1275..>1957)
                     /locus_tag="PLB86_13735"
     CDS             complement(1275..>1957)
                     /locus_tag="PLB86_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRC66254.1"
                     /translation="TLLFLGYPPLMANVSNNMGLVAGGASASWGYRTELTGHGGTLRK
                     LVPLSLLGAVVGATLLLTLPATAFKTIVPVLIVLALVLVIFGPRLQRAAATRHTDQAA
                     GMPPWRTRALAGGVFVGGVYGGYFGAAQGVLLMGLFSALASEPIQRLTGFKNVLVTIV
                     NTVAAIAFLLFAREHVSWPVVGLVAVGSFIGGLLGAVIGRRLPGVVLRSVIVVVGVLA
                     IIKMVWFA"
BASE COUNT          313 a          680 c          699 g          265 t
ORIGIN      
        1 gcagcgtgag gccctggtcg cggcagtggc gcgcgacagc gctgatcgag ccgatcgcca
       61 ggcagtggtc gccgcccagg aggatcggca gccgcccgcc ggacagcgac ccgtagaccg
      121 cgtcgtggac cgcctggttc caggcctgca cctcgggcag gtgccggtaa ccatccaccg
      181 gcggcagcca ggggttgggc ggtccggcga ggttgccgag gtcgttgacg ctcaccccga
      241 actgggcgag cgtctgctgg attccggcga cgcgcatggc ttctggtccc atggacgctc
      301 cgcggtgtgc cgcgccgacg tccgtagggg ctccgatgag atcgactgtc ttcgtggggg
      361 tgctcacggc gagcatcctc acacagtcgc ggccccgctg gcacctatcg cgctcgggcg
      421 cagcgcccag agcgccaccg cgcaggccgc cgcgacgttg agggagtcca ctcccccagc
      481 catcgggatg cggaccgcga ggtcggccgc cgcgatggta tgccgtgtca gcccgtcccc
      541 ttccgtcccc agaaccagcg cgagccgctc cggcgggtcg gccgagagct cgtcgagcgt
      601 catggcggtc tcggtgagcg cgaaggcggc gacggtgaag ccgttctctc gcaagagttc
      661 cggcccgccg agcggccacg gctcgatccg cgtccagggc acctggaaga ccgtgcccat
      721 cgacactcgc acggcgcggc ggtagagcgg gtccgcgcag cgcggggtga caaggacggc
      781 atcgacgccg agggctgcgg cgcttcggaa acaggccccg acgttggtgt ggtcgacgat
      841 gtcttcgagc acgagcactc gccgggcgtt cttgagcagg tcgcccaccg cggggaggac
      901 agggcgctgc atcgccgcca gcgcgccgcg gtgcaggtgg aagcccgtca gcgcctcgat
      961 gacgtcgtgc tcacccacat agatcggtat gccgccctcc cgcgcgtccg cgaccacgtc
     1021 ggcgaggtcg gtgagccagc gctcggccat gaggaacgac cgcgggcggt ggccggccgc
     1081 gagggcccgg cggatgacct tctcggactc ggcgacatag agaccgcgtt cgggctcggt
     1141 gcggcggcgc aacgccacgt ccgtgagcgc cacgtagtcg gccagccgcg ggtcggtcgg
     1201 gtcagtgatc cactcgatgc cgtccgttgc ctcggcgact tttggcacct cggccgccgg
     1261 gaggtccgcg gcactcatgc gaaccacacc atcttgatga tggccagcac gccgacgacg
     1321 acgatgaccg accgcaggac gacacccggt agccgccggc cgatgaccgc accgagcaac
     1381 ccgccgatga acgagcccac ggccacgaga cccaccacag gccaactcac gtgctctcgc
     1441 gcgaagagca agaacgcgat cgctgccacg gtgttgacga tcgtcacgag cacgttcttg
     1501 aatccggtca gccgctggat cggctcgctg gccagcgccg agaacagccc catgagcaga
     1561 actccttgcg ctgcaccgaa atacccgccg tagacgcctc cgacgaagac cccgccggcg
     1621 agtgcgcggg tgcgccaggg cggcataccc gctgcctggt cggtatgccg tgtcgccgcc
     1681 gcccgctgca gtcgcgggcc gaagatgacg agcacgaggg ctaggacgat gaggaccgga
     1741 acgatcgtct tgaaggccgt cgcggggagg gtgagcagca gcgttgcgcc gacgaccgcc
     1801 ccgagcagcg acaacggcac gagtttgcgc agggtgccac cgtggccggt gagttcggtg
     1861 cgatagcccc acgatgcgct cgccccgcct gcgaccaggc ccatgttgtt gctgacgttg
     1921 gccatgaggg gcgggtagcc gaggaagagc aaggtcg
//
LOCUS       DAORPW010000313         1953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_23295_length_1953_cov_717.770811, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000313 DAORPW010000000
VERSION     DAORPW010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23295_length_1953_cov_717.770811"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLB86_13740"
     CDS             complement(<1..694)
                     /locus_tag="PLB86_13740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HRC66255.1"
                     /translation="MGRTLDDARRWASFGTQVCRDAILTLDEDTFAEPSLLPGWTRKH
                     LVAHLAANAEAVRNLATWASTGVPTPMYSSLEQRAADIEAGSHAAGADLAVWFASTAT
                     ALATAWDGLSDGAWQAPVVTAQGRTVPASETPWMRTREVMVHAVDLGTGVTFADLPAD
                     FLEALCDDIVAKRATAPIAGSPDIDICPTDVEGLSWKLPGSEATAPITVTGPLAACTA
                     YLAGRGDSHLSAS"
     gene            complement(694..1539)
                     /locus_tag="PLB86_13745"
     CDS             complement(694..1539)
                     /locus_tag="PLB86_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRC66256.1"
                     /translation="MKLATIRLADGKTAAVRVDGDTLVHLGATDVGSLLAQPDWRSRA
                     AAVAGTTSYAAADATFATLVPTPSKVVCVGLNYKTHIQEMGRDLPEYPTLFAKFADCL
                     IGATDDILRPVETTEFDWEVELAVVIGSTVRRATTEQAEAAIAGFTVLNDITCRDWQF
                     RTREWLQGKNWESSTPVGPYLVTPDELPGGVRPTLAVRCEVDGEVMQSDNTGDLLFDP
                     VALVEYISTMVRLNPGDIIATGTPGGVGHARKPSRYLADGEVVVTEIEGLGRLVNRVV
                     SDGGA"
     gene            complement(1536..>1953)
                     /locus_tag="PLB86_13750"
     CDS             complement(1536..>1953)
                     /locus_tag="PLB86_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HRC66257.1"
                     /translation="DAALTDQLELEREGYAATIEPGHAAIRFLNPSNAGDVTQTIRTQ
                     FHRITTGTETAPRRQTGSSVYQVFDGSGRVSVGDFSWTVGRGDMFVVPSWFPLSIKSE
                     ASASDSDSGALDLFEFGDAPIFEKLNLARTLVEDNR"
BASE COUNT          280 a          609 c          770 g          294 t
ORIGIN      
        1 ggctcgcgct caggtgcgag tcgccacgac cggcgaggta tgccgtgcac gcagccaggg
       61 ggccggtgac ggtgattggg gcggtcgcct cggagcccgg cagcttccag ctgaggccct
      121 cgacgtcggt cgggcagatg tcgatgtcgg gcgaaccggc gatcggtgcc gtggcccgct
      181 tggcgacgat gtcgtcgcac agtgcctcga ggaagtcggc cggcaggtcg gcgaaggtga
      241 cgccggtgcc gagatcgacg gcgtggacca tgacctcgcg ggtgcgcatc cacggggttt
      301 cggacgcggg gaccgtgcgg ccctgggcgg tgacgaccgg tgcctgccag gcgccgtcgg
      361 acagtccgtc ccacgccgtg gcgagcgcgg tggccgtgct ggcgaaccag accgccaggt
      421 cggcgccggc ggcgtggctg ccggcctcga tgtcggcggc ccgctgctcg agcgaggaat
      481 acatcggggt cgggacgccg gtcgacgccc acgtcgcgag gttgcggacg gcctcagcgt
      541 tggcggcgag gtgcgcgacg aggtgcttac gggtccagcc cggcaacagg ctgggctcgg
      601 cgaaggtgtc ctcgtcgagc gtgaggatcg cgtcgcggca tacctgagtg ccgaaggagg
      661 cccagcggcg ggcgtcgtcg agcgtgcggc ccatcaggca ccaccgtcac tgaccacgcg
      721 gttgaccagc cggcccaagc cctcgatctc ggtgacgacg acttcgccgt cggccaggta
      781 acgcgagggc ttgcgggcgt ggccgacccc gccgggggtg ccggtggcga tgatgtcgcc
      841 ggggttgagc cggaccatcg tggagatgta ttcgaccagg gcaaccgggt cgaagagcag
      901 gtcgccggtg ttgtcggact gcatgacctc accgtcgacc tcgcaccgga cggcgagcgt
      961 cgggcggacg ccgccgggca gctcgtcggg ggtgacgagg taggggccga cgggggtcga
     1021 ggactcccag ttcttgccct gcagccactc gcgggtgcgg aactgccagt cacggcaggt
     1081 gatgtcgttg agcaccgtga agccggcgat tgcggcctcg gcctgctcgg tcgttgcgcg
     1141 ccggaccgtg ctgccgatga cgacggccag ctcgacctcc cagtcgaact cggtcgtctc
     1201 gaccggacgg aggatgtcgt cggtcgcccc gatgaggcag tcggcgaact tcgcgaagag
     1261 cgtggggtat tcgggcaggt cccggcccat ctcctggatg tgggtcttgt agttgaggcc
     1321 cacgcacacc accttggacg gcgtcgggac gagcgtcgcg aaggtggcgt cagcggcggc
     1381 atacgacgtg gtgcctgcga ccgcggcggc acgtgagcgc caatccggct gggccagaag
     1441 cgaacccacg tctgtcgccc cgaggtgaac gagggtgtct ccgtcgacgc ggaccgcagc
     1501 ggtcttgccg tcggccaggc ggatggtggc gagcttcatc ggttgtcctc cacgagggtg
     1561 cgggcgaggt tgagcttttc gaagatgggg gcgtcgccga actcgaacag gtcgagggct
     1621 ccggagtcgg agtcggaggc cgaggcttcg gacttgatcg acagcgggaa ccacgagggc
     1681 acgacgaaca tgtcgccgcg gccgacggtc caggagaagt caccgaccga gacccggccg
     1741 gagccgtcga acacctgata gaccgacgag ccggtctggc ggcgcggggc ggtctcggtg
     1801 ccggtggtga tccggtggaa ctgggtgcgg atcgtctgcg tgacgtcccc ggcgttgctc
     1861 gggttgagga agcggatggc ggcgtgcccc ggctcgatcg tcgcggcata cccctcgcgc
     1921 tccagctcga gctggtcggt gagggcggcg tcg
//
LOCUS       DAORPW010000314         1945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_23463_length_1945_cov_712.322751, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000314 DAORPW010000000
VERSION     DAORPW010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23463_length_1945_cov_712.322751"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLB86_13755"
     CDS             <1..459
                     /locus_tag="PLB86_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HRC66258.1"
                     /translation="CSKGNLDMAYPGLILANAALGEGIEVHLFFTFWGFDIINKETMG
                     NLKFSYIGNTAMHMPGHFGMSMPQGMAGLPGVTAMTTKMMKKQISDLDVPDVPEFLEL
                     LHGSGAKMWACRMSADMMHLELEHLYDGVEAIISATDFIELTDGAQMLFI"
     gene            608..>1945
                     /locus_tag="PLB86_13760"
     CDS             608..>1945
                     /locus_tag="PLB86_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRC66259.1"
                     /translation="MEAPVSTLAATDVVALVGTTEASPAAAALAVIEVVERMKSHLDA
                     LVLPAWRMLHAAYVEALESPERPPMTARERERETSVARSGAVDEVMAATGLGESECRR
                     RLGLALADPVRVDSLVRLLAAGELALWQVLGLHDDTADLDPTAADEVVQVVSAPTRAG
                     DRPSAQLRRRRLRRELARRGAQSACGSRRLAARDARAELEPDGTGRLWITGEGGRVVA
                     AIERVDRLARGIRASGQHPDRTLAQLRSDVALDLILHTTSVEGAEWSRLGDLPPAQVQ
                     VVIGLDVLSGATDGVAEIPGHGWVPADVARDLAHTPGSVWQRLVIDPATGALVELSST
                     RYTPPARLRAHVVARDRVCRAPGCVVPATTCDLDHDIPWPMGVTSADNLSAKHRRHHQ
                     LKTVGLWSSRHDPTTDALTWTTLAGRAYRTDPFDYREVLDADLDDGPLVRRRGA"
BASE COUNT          284 a          697 c          662 g          302 t
ORIGIN      
        1 tgctccaagg gcaacctcga catggcctac cccggcctca tcctcgccaa cgccgccctc
       61 ggcgaaggca tcgaggtgca cctgttcttc accttctggg gcttcgacat catcaacaag
      121 gagacgatgg gcaacctgaa gttctcctac atcggcaaca cggcgatgca catgcccggc
      181 cacttcggca tgtcgatgcc gcagggcatg gctggcctgc cgggcgtcac cgccatgacg
      241 accaagatga tgaagaagca gatctccgac ctcgacgtcc ccgacgtccc ggagttcctc
      301 gagctcctgc acggctccgg cgccaagatg tgggcctgcc ggatgagcgc cgacatgatg
      361 cacctcgagt tggagcacct ttacgacggg gtcgaggcga tcatctcggc caccgacttc
      421 atcgagctca ccgacggggc acagatgctg ttcatctgag gtcgtcccat gcgggaggta
      481 tgccgcgcgc tcaccgtgag cgggcggcat acctgcgtct gggttgggcc gaggttggca
      541 gcaattcgct tgtcccgcaa ttcgattcct cttgtcatcg aacgggtgtt cgggtagaat
      601 cgaggacatg gaagcccccg tcagcaccct cgccgcgact gatgtcgtgg cgttggtcgg
      661 caccacggag gcgtctccgg cagccgccgc gctggcggtg atcgaggtcg tcgagcggat
      721 gaagtcgcat ctggacgccc tggttctgcc ggcctggcga atgctccacg cggcatacgt
      781 cgaggcgttg gagtcgccgg agcgaccgcc gatgaccgcc cgcgagcgag agcgcgagac
      841 gtccgtcgcc cgatccggag cggtcgacga ggtcatggcc gcgaccgggc tcggggagtc
      901 ggaatgccgt cggcgcctcg ggctggccct ggccgatccg gtgcgggtcg actccctcgt
      961 ccggctgctg gccgccgggg agttggcgct ctggcaggtg ctcgggctgc acgacgacac
     1021 tgccgacctg gacccgaccg cggccgacga ggtggtgcag gtcgtgtccg cgccgacccg
     1081 tgcgggtgat cgcccgtcgg cccagcttcg gcgacgccgg ctgcggcgcg agttggctcg
     1141 gcggggcgcc caatccgcct gtggatcacg gcgactcgcg gcgcgggacg cgcgcgcgga
     1201 gcttgagccc gacggcacgg gccgcctctg gatcaccgga gaaggcggcc gcgtcgtggc
     1261 ggcgatcgag cgggtcgacc ggttggcccg cgggatccgg gcgtccggcc agcaccccga
     1321 tcgcacgctc gcccaactgc gctccgacgt cgccctcgac ctcatcctgc acacgacctc
     1381 cgtcgagggc gccgagtgga gccggttggg tgacctcccg ccggcgcagg tgcaggtggt
     1441 catcggcctc gacgtcctct ccggtgcgac cgacggcgtg gccgagatcc cgggccatgg
     1501 ttgggtgccc gccgacgtgg ctcgcgacct cgcgcacaca ccgggcagcg tctggcagcg
     1561 gctcgtcatc gaccccgcca ccggggcgtt ggtcgagttg tcgtcgaccc gctacacgcc
     1621 gccggcccgg ttgcgcgcgc acgtggtggc ccgcgatcgg gtatgccgtg cccccggatg
     1681 cgtcgtcccg gccacgacgt gcgacctcga ccacgacatc ccttggccga tgggcgtcac
     1741 ctcggccgac aacctcagcg ccaagcaccg ccgacatcac caactcaaga ccgtcggcct
     1801 ctggagcagt cgtcatgacc ccacgacgga cgcgctcacc tggaccaccc tcgcgggccg
     1861 cgcctatcgc accgacccct tcgactaccg cgaggtgctc gacgctgacc tcgatgacgg
     1921 accgctcgtg cgccgccggg gcgca
//
LOCUS       DAORPW010000315         1913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_24134_length_1913_cov_564.973089, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000315 DAORPW010000000
VERSION     DAORPW010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24134_length_1913_cov_564.973089"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1061)
                     /locus_tag="PLB86_13765"
     CDS             complement(12..1061)
                     /locus_tag="PLB86_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66260.1"
                     /translation="MTSRLARSALASISLLLAGCTAMSSSPTSTSATDPASGSPTASA
                     TAAADLGSQLAQQILRDYNVRNNPAIVASAAGDPTGWKAADTGITLEQDLFGTVDARV
                     NKTLKPASPFDFTPKELIVASPEGAYPRWAVIEFGESERAGGTASPSATASASPADRR
                     VVGVFVQPVADAPWLMENHITVPRPATSATAREASAAESADARAALQRALDYLTFGRE
                     APEVDLGSIPGSRSSLLIPAKDNIRDTTLVCSTYVPPAGVPGYGNSRAFAVEDTVVLI
                     GSISCSASVMLKRSTTTNAWQRAILGAAETTKGVTFSYVGTIVVSTTPGSRPTVSWWR
                     LSDALAPAVMVRERG"
     gene            complement(1124..1339)
                     /locus_tag="PLB86_13770"
     CDS             complement(1124..1339)
                     /locus_tag="PLB86_13770"
                     /inference="COORDINATES: protein motif:HMM:NF039372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6191 domain-containing protein"
                     /protein_id="HRC66261.1"
                     /translation="MIFGREGASAGGAVFGEAIALLQPGYAHTLAEKRRQESSREVPE
                     DGAPPLIDLEAGVIRITRPPAAEPPAP"
     gene            complement(1446..>1913)
                     /locus_tag="PLB86_13775"
     CDS             complement(1446..>1913)
                     /locus_tag="PLB86_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRC66262.1"
                     /translation="KVPVDKIGEVIGPKGKMINQIQDDTGADISIEDDGTVYIGAVDG
                     PSAEAARAAINAIANPQMPEVGERFMGTVVKTTTFGAFISLLPGKDGLLHISEVRKLV
                     GGKRIDNVDDVLKIGQKVQVELKEIDPRGKLSLAAVMADGEGDKADAEAPAEG"
BASE COUNT          291 a          648 c          660 g          314 t
ORIGIN      
        1 ctgactgccg gtcagccccg ctcgcgaacc atcaccgccg gggcgagggc gtcactcagc
       61 cgccaccacg agaccgtcgg tcgactcccg ggtgtcgtgc tgacgacgat cgtcccgacg
      121 taactgaacg tcacgccctt ggtcgtctcg gcggcgccga gaatggcccg ctgccaggcg
      181 ttggtggtcg tgctccgctt gagcatcacg cttgccgagc aggagatcga gccgatcaga
      241 accacggtgt cctcgacagc gaaggcccgg ctgttgccgt agccaggaac accggccgga
      301 gggacgtagg tcgagcacac cagcgtcgtg tccctgatgt tgtccttggc cgggatcaac
      361 aggctcgatc gagagccggg gatcgacccg aggtcaacct ctggggcttc ccgtccgaag
      421 gtgagatagt ccagcgcccg ttgcagggcg gcccgggcat cggctgactc cgcggccgaa
      481 gcctccctcg ctgtcgccga ggtggcgggg cgcggcaccg tgatgtggtt ctccatgagc
      541 cagggcgcgt cggctacggg ctggacgaac accccaacca ctcggcggtc ggccggggag
      601 gccgacgccg tcgcgctggg gctggccgtg ccacccgctc gttccgattc gccgaactcg
      661 atcaccgccc accggggata ggccccctcc ggcgatgcga cgatcaactc cttgggggtg
      721 aagtcgaacg gcgatgccgg cttgagggtc ttgttcactc gcgcatcaac agtcccgaac
      781 aggtcctgtt cgagggtgat ccccgtgtcg gcggccttcc acccggtggg gtcgcccgcc
      841 gcggacgcaa cgatcgccgg gttgttgcgg acgttgtagt cccgcaggat ctgctgcgcg
      901 agctgcgaac cgaggtctgc cgcggcggtg gccgaggcag tcggcgaacc ggaggccggg
      961 tcggtggccg atgtcgaggt cggcgaagac gacatcgcgg tgcatcccgc gagcaggaga
     1021 ctgatcgaag cgagggcact gcgtgccagg cgtgaggtca tggttcgagc agatcacgat
     1081 ccccaccaac cccgcagagc tcatccacag ccctgtggat aactcagggc gctggtggtt
     1141 cagccgctgg tggccgggtg atccggatga ccccggcctc aaggtcgatc agcggcgggg
     1201 caccgtcctc gggcacctca cgggaggact cctgacggcg cttctcggcc agggtgtggg
     1261 cgtagcccgg ctgcagcaac gcgatggctt cgccgaacac ggcgccaccc gccgacgcac
     1321 cctcccgacc gaagatcacc tctccagggt aaccgcgggc aggcgcgcac gtcaccgcaa
     1381 acgccggtgg ggcggcatac gggagatccc gtatgccgcc ccaccgggga cgtgcgtggt
     1441 gtggctcagc cctcggcagg agcctcggcg tcggccttgt cgccctcgcc gtcggccatg
     1501 acagcagcca gcgacagctt gccgcgcggg tcgatctctt tgagctcgac ctggaccttc
     1561 tggccgatct tgaggacgtc gtcgacgttg tcgatccgct tgccaccgac aagcttgcgc
     1621 acctcggaga tgtgcagcag accgtccttg cccgggagca aggagatgaa cgccccgaag
     1681 gtcgtcgtct tgacgacggt gcccatgaag cgctcgccga cctcgggcat ctgcgggttg
     1741 gcgatcgcgt tgatcgcggc ccgcgccgcc tcggccgagg ggccgtcgac tgcgccgatg
     1801 tagacggtgc cgtcgtcctc gatggagatg tccgcgccgg tgtcgtcctg gatctggttg
     1861 atcatcttgc ccttcgggcc gatgacctcg ccgatcttgt cgaccggcac ctt
//
LOCUS       DAORPW010000316         1906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_24267_length_1906_cov_764.378174, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000316 DAORPW010000000
VERSION     DAORPW010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24267_length_1906_cov_764.378174"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..216
                     /locus_tag="PLB86_13780"
     CDS             19..216
                     /locus_tag="PLB86_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043806102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HRC66263.1"
                     /translation="MASQEQSRPQRREGDPDAPPPEVPVGGAQTQVSNADIDSVLDEI
                     DEVLEVNAEEFVRSFVQKGGQ"
     gene            213..1055
                     /gene="prcB"
                     /locus_tag="PLB86_13785"
     CDS             213..1055
                     /gene="prcB"
                     /locus_tag="PLB86_13785"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HRC66264.1"
                     /translation="MNRSTPPPDGRAGSLPAAYLRPGSASFTEFVAGVAPELLPGRRP
                     LPAGMSLEAPHGTTIVAAEFAGGVVVAGDRRATMGNLIANRDMEKVYAADEYSVVGIA
                     GTAGIAVELVKLYQVELEHYEKIEGTIMSLEGKANRLASMIRGNLGLAMQGLTVVPMF
                     AGFDLDERRGRIFSYDVTGGCYPERDHHSIGSGSLFARGSLKKLWRPGLSEADTVRAC
                     LEALYDAADDDSATGGPDLGRRIWPTLAVLDETGVRFLSDDDLAVRVEAIVADRFGNP
                     GGAR"
     gene            1084..1902
                     /gene="prcA"
                     /locus_tag="PLB86_13790"
     CDS             1084..1902
                     /gene="prcA"
                     /locus_tag="PLB86_13790"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HRC66265.1"
                     /translation="MSMPFYVSPEQLMKDKADYARKGIARGRSVVVLQYDRGIVFVAE
                     NPQSLRKISEIYDRIAFAAVGMYYEFENLRVAGIRYADLRGYSYDRSDVSARGLANAY
                     AQTLGTVFTQEPKPYEIEICVAEVGATPERDQIFRLTYDGSVADQHGFVVMGGSNQAA
                     EERLKAGWQEGMPLADALILAVAALAADPAGGEPRTLSPDQLEVAVLDRNRPRRTFRR
                     IVGPLLADLLNVDSPTSHAPEFDEDVPGHHDTLSGQPAAHPDHDDDGNQGEDSR"
BASE COUNT          306 a          671 c          659 g          270 t
ORIGIN      
        1 cgagcaggtg gaggacccat ggccagccag gagcagagcc gtccgcagcg gcgcgagggt
       61 gaccccgacg cgccgcctcc cgaggttccc gtcggcggcg cgcagaccca ggtgtccaac
      121 gccgacatcg acagcgtcct cgacgagatc gacgaggtgc ttgaggtcaa cgccgaggag
      181 ttcgtccgca gcttcgtcca gaagggcggc cagtgaaccg cagcacccct ccgccggatg
      241 ggcgcgctgg gagtctccca gcggcatacc tgaggccggg gagcgcgtcg ttcaccgagt
      301 ttgtcgcggg cgtggcccct gagttgttgc ctggtcggcg tcccctgccc gccgggatgt
      361 ccctggaggc gccgcacggc acgaccatcg tcgcggccga gttcgccggc ggagtggtcg
      421 tcgcggggga ccggcgcgcg acgatgggca acctcatcgc caaccgcgac atggagaagg
      481 tgtatgccgc ggacgagtac tccgtcgtcg gcatcgccgg gacggccggg atcgccgtcg
      541 agctggtcaa gctctatcag gtcgagttgg agcattacga gaagatcgag ggcacgatca
      601 tgtccctcga aggcaaggcc aaccggctgg cctcgatgat ccgcggcaac ctcgggttgg
      661 ccatgcaggg gctcaccgtc gtgccgatgt tcgccgggtt cgacctcgac gagcggcgcg
      721 ggcggatctt ctcctacgac gtgaccgggg gctgctaccc cgagcgtgat caccacagca
      781 tcggctccgg gtcgctcttc gcccgcggat cgctcaagaa gctctggcga ccggggctgt
      841 ccgaggccga caccgtgcgg gcctgcctgg aggcgctcta cgacgccgcc gacgacgact
      901 cggcgaccgg gggaccggac ctcgggcggc ggatctggcc gaccctggcg gtgctggacg
      961 agaccggagt gcgcttcctc agtgacgacg acctggccgt ccgggtcgag gccatcgtcg
     1021 ccgaccgctt cggcaacccg gggggcgcgc gatgagcgcc gcccagcgag caggaggtgc
     1081 gcgatgagca tgccgttcta cgtctcaccc gagcaactga tgaaggacaa ggccgactac
     1141 gcgcgcaagg gcatcgcccg cggccgctcg gtcgtcgtgc tgcagtacga ccggggcatc
     1201 gtcttcgtcg cggagaaccc gcagagcctg cgcaagatct ccgagatcta cgaccgcatc
     1261 gcgttcgccg ccgtcggcat gtactacgag ttcgagaacc tgcgggtcgc cggcatccgg
     1321 tatgccgatc tgcgcggcta ctcctacgac cgctccgacg tgtcggcgcg agggctggcc
     1381 aatgcgtacg cccagaccct ggggacggtt ttcacccagg agcccaagcc ctacgagatc
     1441 gagatctgcg tcgccgaggt gggtgcgacc cccgagcgcg accagatctt ccgactcacc
     1501 tacgacgggt cggtcgccga ccagcacggg ttcgtcgtca tgggcgggtc aaaccaggca
     1561 gcggaagagc gcctcaaagc cggttggcag gagggtatgc cgctcgccga cgccctcatc
     1621 ctcgccgtcg cggccctcgc ggccgacccc gccgggggag agcctcgaac cctcagcccc
     1681 gatcagctcg aagtggccgt cctggaccgc aaccggcccc ggcggacctt ccggcggatc
     1741 gtcggtccgc tgttggccga cctgctcaac gtcgacagcc cgacctcgca tgcgcccgag
     1801 ttcgacgagg atgtgcccgg ccaccacgac accctgtccg gccagcccgc ggcccacccc
     1861 gaccacgacg acgacggcaa ccagggcgag gacagccgct gattcg
//
LOCUS       DAORPW010000317         1886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_24713_length_1886_cov_709.033315, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000317 DAORPW010000000
VERSION     DAORPW010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24713_length_1886_cov_709.033315"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1886
                     /locus_tag="PLB86_13795"
     CDS             <1..>1886
                     /locus_tag="PLB86_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRC66266.1"
                     /translation="TSRDIPGFVESFAGSDHLVSEYLLHEVIDEQEPAVRQFLLQTSV
                     LDWLSADLCNAVTGLEIAAGMLDGLFHRSMFLIPLDEAGTTFRYQHLFADALRSRLRI
                     ENPHSVSALHHRAARWLLAHGHEEEAVGHLLDAGDPAGAFRVISRIGHHFVERGEAAT
                     LVRWLSTIAAEDPDGPVEVEISLLAAQLAAGEGTAATETYRRLSRRPNLTLGERTAAD
                     ALSAFLVFRDLPPPQVLQVTDRVRVALQGLDPDEVVDFLGIGGADSVQAVAEYTSGLA
                     HFLAGDLDRAADVLERARTLPGTRYVVWHVYVLSTLALVRAWQGRCAQAMGLARSAIE
                     EARSVRLSDHLGSTHAHLALALVHLDRVEPDDARRCLADAQVHVRGRVSAGINADLEH
                     VLQARLIALQDGPSAALDHLRSPLAAGSEATLVREERHALLAHLLIATGDLDGARTVA
                     AGIPESAVLAPARVDLALATGCVAAARAALDGWTTAVGDVRAEVCRRLHEFRVLRAEG
                     DQRGADRSLREAIAAARAEGLRWPFLEVPAALRVVRRGSGATSWLSDAAFGKVVARLE
                     PGASQQQGLIEPLTPRELDVLAYLPGRMMHHEIAADLYVSVNTVKTHLSSIYRKLGVT
                     ERNAA"
BASE COUNT          250 a          681 c          651 g          304 t
ORIGIN      
        1 acctctcggg acatcccggg gttcgtcgag tcgttcgccg gcagtgacca cctcgtctcc
       61 gagtacctgt tgcacgaggt gatcgacgag caggagccgg ccgtgcgcca attcctcctg
      121 cagacctccg tcctcgactg gctgtcggcc gatctgtgca acgccgtcac gggcctggag
      181 atcgccgcgg gaatgcttga cgggctcttt caccggtcga tgtttctcat ccccctggac
      241 gaggccggca cgaccttccg ctatcagcac ctcttcgccg acgcgctgcg atcccggctg
      301 cggatcgaaa acccgcactc ggtgagcgcc ctgcaccacc gggccgcccg ctggctgttg
      361 gctcacgggc acgaggagga agcggtcggc catctgctcg atgccggcga cccggccggg
      421 gcgttccgag tcatctcccg catcggccac cacttcgtcg agcggggtga ggccgcaacc
      481 ctcgtgcgtt ggctgagcac catcgctgcc gaggatcccg atggcccggt cgaggtcgag
      541 atcagcctgc tcgccgccca gttggctgcg ggtgagggga cggcagcgac cgagacttat
      601 cgccggctca gccgccgtcc caatctcacc ctgggggagc ggaccgcagc cgacgcgctc
      661 tctgccttcc tggtcttccg cgacctgccg ccgccgcagg tcctgcaggt cactgatcgg
      721 gtgcgcgtcg ccctgcaggg gctcgaccct gatgaggtcg tcgacttcct tggcatcggg
      781 ggagccgact ccgtccaggc cgtcgccgaa tacacctcgg gtctggcgca cttcctcgcc
      841 ggcgacctcg accgcgcggc tgacgtgttg gagcgcgctc ggacgctgcc cgggacccgt
      901 tacgtcgtct ggcacgtcta cgtgttgagc accttggcct tggtccgcgc ctggcagggc
      961 cgctgcgcgc aggccatggg gctggcgcgg tccgcgatcg aggaggcccg gtcggtccgg
     1021 ctgtccgatc acctggggtc gacgcatgcg cacttggccc tcgcgctggt ccacctcgac
     1081 cgcgtcgagc cggacgacgc gcggcgctgc ctcgccgacg cccaagtcca tgtgcgcgga
     1141 cgcgtgagcg cgggcatcaa cgccgacctg gaacacgtgc tgcaagcccg gctgatcgca
     1201 ctgcaggacg ggccgagcgc tgctctcgac cacctgcgct cgccgctcgc cgcaggcagt
     1261 gaggccaccc tcgtgcgcga ggagcggcac gcgctgctcg cgcatctcct catcgccacc
     1321 ggggacctgg acggcgcgcg gaccgtggcg gccggcatac cggaaagcgc ggtgctggcc
     1381 cccgcgcgag ttgatctggc ccttgccacg gggtgcgtcg cggcggcgcg ggctgccctc
     1441 gacgggtgga ctaccgcggt gggtgacgtg cgagctgagg tatgccgtcg gctgcacgag
     1501 ttccgcgtgc tgcgcgccga gggcgaccag cggggtgccg acaggtcgtt gcgggaggcc
     1561 atcgcggcag cgcgcgccga gggtctgcgc tggcccttcc tcgaggtgcc ggctgccctg
     1621 cgcgtggttc gccgaggcag cggtgcgacc tcgtggctca gcgatgctgc gttcgggaag
     1681 gtcgtcgccc gcttggaacc gggcgcaagc cagcagcagg gcctgatcga accgctcacc
     1741 cctcgcgagc tcgatgtcct cgcctacctg ccggggcgga tgatgcacca cgagatcgcc
     1801 gccgacctct atgtgtcggt caacaccgtc aagacgcacc tgagcagcat ctatcgcaaa
     1861 ctcggcgtca ccgagcgcaa cgccgc
//
LOCUS       DAORPW010000318         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_24905_length_1877_cov_739.361690, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000318 DAORPW010000000
VERSION     DAORPW010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24905_length_1877_cov_739.361690"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLB86_13800"
     CDS             complement(<1..440)
                     /locus_tag="PLB86_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HRC66267.1"
                     /translation="MTSNLLGMPATLLPVDPAADIVAGLREGPGHGSPDHAGSAAEGM
                     PHAALESVAAAYPTSSLAWALLAEGALDADHPVTAYAYARTGYHRGLDSLRKAGWRGQ
                     GPIPWEHEPNRGFLRALAALARAARVIGETEEEARCAGFLRESSR"
     gene            complement(443..1474)
                     /gene="fbaA"
                     /locus_tag="PLB86_13805"
     CDS             complement(443..1474)
                     /gene="fbaA"
                     /locus_tag="PLB86_13805"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRC66268.1"
                     /translation="MPIATPEVYADMLDRAKAGAFAYPAINITSSQTINAAIRGFAEA
                     GSDGIIQVSTGGADFASGSNIKNMVTGASALAAFAAEVAKNYSVNIALHTDHCPKDKL
                     DGFVRPLLAISAERVKAGLPPLFQSHMWDGSAVPLAENLTIAQDLLALAKAAHIILEV
                     EIGVVGGEEDGVANEINEQLYTTPEDALATVEALGLGENGRFMAALTFGNVHGVYKPG
                     NVKLRPEILRQCQDAVVAAKGLAAGSKPLDLVFHGGSGSLPQEISDAVDFGVIKMNVD
                     TDTQYAFTRPVAGWMLKNYDGVLKVDGEVGNKKQYDPRAWGKEAEAAMAARVVRACQD
                     LRSAGTHQA"
     gene            complement(1612..>1877)
                     /locus_tag="PLB86_13810"
     CDS             complement(1612..>1877)
                     /locus_tag="PLB86_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRC66269.1"
                     /translation="LAPDETDPTPPGMPPAATDPAATHADRTTDSDGWIRMTLCFRGE
                     MGAVAALLAFGGDAEIVHPPSTRRLMAQTARAALARHTGEEPA"
BASE COUNT          254 a          623 c          687 g          313 t
ORIGIN      
        1 cgggatgact cgcgcaggaa gccggcgcag cgggcctctt cctcggtctc gccgatgacg
       61 cgggccgctc gggccagcgc cgcgagcgcc cgcaggaacc cccggttggg ttcgtgctcc
      121 cacgggatcg gtccctgacc acgccacccg gccttgcgca gcgagtccag accgcggtga
      181 taacccgtgc gggcatacgc gtatgccgtg accgggtggt ccgcgtcgag cgccccctcg
      241 gccagcaggg cccaggccaa cgacgaggtc gggtatgccg cggcaacgga ttcgagggcc
      301 gcgtgcggca taccctcagc ggcgcttccc gcatggtctg gcgacccgtg gccgggcccc
      361 tcacggagcc cggccacgat gtcagccgcg gggtcgaccg gcagtagcgt cgccggcata
      421 cccagcaggt ttgaggtcac cctcaggcct ggtgcgttcc ggccgagcgc aggtcctggc
      481 aggcgcggac gacgcgggcg gccatggcgg cctcggcctc cttgccccag gcgcgcgggt
      541 cgtactgctt cttgttgccg acctcgccgt cgaccttgag gacgccgtcg tagttcttga
      601 gcatccagcc ggcgaccggg cgggtgaagg cgtactgcgt gtcggtgtcg acgttcatct
      661 tgatgacgcc gaagtcgacc gcgtccgaga tctcctgcgg gagcgagccg gagccgccgt
      721 ggaagaccag gtccagcggc ttgctgccgg ccgcgaggcc cttggccgcg acgaccgcgt
      781 cctggcactg gcggaggatc tcggggcgca gcttgacgtt gcccggcttg tagacgccgt
      841 gcacgttgcc gaaggtgagg gcagccatga agcggccgtt ctcgccgaga ccgagggcct
      901 cgaccgtcgc gagggcgtcc tcgggggtcg tgtagagctg ctcgttgatc tcgttggcga
      961 cgccgtcctc ttcgccgccg acgacaccga tctcgacctc aaggatgatg tgcgcggcct
     1021 tggcgagggc cagcaggtcc tgggcgatgg tgaggttctc ggccagcggc acggccgagc
     1081 cgtcccacat gtgcgactgg aacagcgggg gcaggccggc cttgacccgc tcggccgaga
     1141 tggccagcag cggacggacg aagccgtcga gcttgtcctt cgggcagtgg tcggtgtgca
     1201 gcgcgatgtt gaccgagtag ttcttggcga cctcggcggc gaacgcggcg agggcgctgg
     1261 cgccggtcac catgttcttg atgttggagc ccgacgcgaa gtcggcgcca ccggtggaca
     1321 cctggatgat gccgtcggag ccggcctcgg cgaagccacg aatcgccgcg ttgatggtct
     1381 ggctggaggt gatgttgatc gccgggtagg caaaggcgcc agccttcgcg cggtcgagca
     1441 tgtcggcgta aacctcaggt gttgcgatgg gcatggggcg tctcctaggc gctcggcggt
     1501 cggtccgccc ccatccttcc acttcctggc aagcgcttgc ccagggacgt tcgccccgaa
     1561 cacgtcggct acctctgcgt caccccacgt tcacctcacc aacgctgggc gtcacgccgg
     1621 ttcttctccc gtatgccgtg ccagcgcggc tctcgcggtc tgcgccatga gccgccgggt
     1681 cgatggtggg tggacaatct cggcgtcgcc gccgaacgcc aatagtgctg cgacagcgcc
     1741 catttcgcca cggaagcaca gggtcatgcg gatccagccg tcggagtcgg tggtcctgtc
     1801 agcgtgggtg gctgcggggt cagtggctgc gggcggcata ccgggtggtg tggggtcggt
     1861 ctcgtccggg gcgagga
//
LOCUS       DAORPW010000319         1846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_25615_length_1846_cov_885.977108, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000319 DAORPW010000000
VERSION     DAORPW010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25615_length_1846_cov_885.977108"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /gene="cysK"
                     /locus_tag="PLB86_13815"
     CDS             <1..807
                     /gene="cysK"
                     /locus_tag="PLB86_13815"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRC66270.1"
                     /translation="VKDRIGVAIIDAAEASGELPPGGTIVEATSGNTGIALAMVGAAR
                     GYNVVLTMPESMSKERRALLRAFGAELILTDPSTGMKGAVTKAEEVAAERKGAVLARQ
                     FANAANPEIHRRTTAEEIWADTDGGVDIVVAGIGTGGTITGVGQVLKARKPGVQIVAV
                     EPAESPILNGGAPGPHKIQGIGANFVPEILDTTVYDEVIDVNAGQAVDYARRAATQEG
                     LLVGISSGAALYAAGLVAARPENAGKTIVVIIPSFGERYLSTVLFEGLVD"
     gene            986..1546
                     /gene="cysE"
                     /locus_tag="PLB86_13820"
     CDS             986..1546
                     /gene="cysE"
                     /locus_tag="PLB86_13820"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRC66271.1"
                     /translation="MIREDIDAAMLRDPAAHSRVEMFLASSGLHAIWAYRVAHSLWGN
                     PSTRLAARLLSQIVRAMTGVEIHPGATIGRRFFIDHGMGVVIGETTEIGDDVMLYHGV
                     TLGGRSLSKTKRHPTLGNRVTVGAGAKVLGPILLGDDSQIGANSVVVKDVPAGYVAVG
                     MPAQVRPAVAGLTPEEMLDHNPAIWI"
     gene            complement(1655..>1846)
                     /locus_tag="PLB86_13825"
     CDS             complement(1655..>1846)
                     /locus_tag="PLB86_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66272.1"
                     /translation="GVRTVVAAVSRIPDELAAGAMTAYHRLLVTGIDSATALAQATAD
                     LPTVARAFTCFGADWRAAT"
BASE COUNT          274 a          682 c          631 g          259 t
ORIGIN      
        1 gtcaaggacc gcatcggcgt ggcgatcatc gacgccgccg aggcgtccgg cgaactgccg
       61 cccggcggca ccatcgtcga ggccacctcg ggcaacacgg gcatcgccct ggcgatggtc
      121 ggcgccgccc gcggctacaa cgtcgtcctg acgatgcccg agtcgatgtc caaggagcgc
      181 cgggccctgc tgcgcgcctt cggtgccgag ttgatcctca ccgacccgtc gaccggcatg
      241 aagggcgccg tcaccaaggc cgaagaggtc gccgccgagc gcaagggcgc cgtgctcgcc
      301 cgccagttcg ccaacgccgc gaaccccgag atccaccgcc gcacgacggc cgaggagatc
      361 tgggccgaca ccgatggtgg cgtcgacatc gtcgtcgcgg gcatcggcac cggtggcacg
      421 atcaccggag tcggccaggt gctcaaggcc cgcaagcctg gtgtccagat cgtcgccgtc
      481 gagccggccg agtcgccgat tctcaacggt ggagcgcccg gcccgcacaa gatccagggc
      541 atcggcgcca acttcgtccc ggagatcctc gacaccaccg tctacgacga ggtcatcgac
      601 gtcaacgccg ggcaggccgt cgactacgcc cgccgggccg ccacccagga gggcctgctc
      661 gtcggcatct cctccggcgc tgccctgtat gccgcaggcc tggtcgccgc gcgccccgag
      721 aacgccggca agaccatcgt cgtcatcatc cccagcttcg gggagcgcta cctctccacg
      781 gtcctcttcg agggcctcgt cgactgaacg gcataccgcc atgctcgccg cactgaccag
      841 caccctgcgc cgtgaccgcg gccgcgaccg gtcgtgggag ctcaccgccg accccgacac
      901 gggcctcacg tcccgggtcg agccggtggg gccgcccgga ccggtcgccg cgccgcgccg
      961 gcacatcgtg cgccggacct gggcgatgat ccgtgaggac atcgacgcgg cgatgctgcg
     1021 cgatccggcg gcgcactcgc gggtcgagat gttccttgcg tcgtcggggc tgcacgccat
     1081 ctgggcctat cgggtggcgc actcgctgtg gggcaacccg tcgacccgac tggccgcgcg
     1141 gttgctctcg cagatcgtgc gggcgatgac cggtgtcgag atccaccccg gcgcgacgat
     1201 cgggcggcgg ttcttcatcg accacggcat gggcgtcgtc atcggcgaga ccaccgagat
     1261 cggcgatgac gtcatgctct atcacggcgt cacgctcggt ggccggtcgc tgtcgaagac
     1321 caagcgccac cccaccctgg gcaaccgggt cacggtcggg gccggcgcga aggtcctcgg
     1381 cccgatcctc ctcggcgacg actcccagat cggcgccaac tcggtggtcg tcaaggacgt
     1441 ccctgcgggc tatgtcgccg tcggcatgcc ggcgcaggtg cgccccgccg ttgcgggcct
     1501 cacgcccgag gagatgctcg accacaaccc ggcgatctgg atctgagcgt cggtcgctac
     1561 ccagccggtt cgaggtattc ggccgtcgcc agatgatggt ggattacctc gaatcggcag
     1621 ggcggggcgg ggcaaggtgg ggcgtgaggg caactcaggt cgcggcgcgc cagtcggctc
     1681 cgaaacacgt gaaggcccgc gccaccgtcg gaaggtcggc ggtcgcctga gccagcgccg
     1741 tggcactgtc gatcccggtc accaacagcc ggtggtatgc cgtcatcgcc cctgccgcga
     1801 gctcgtccgg gatgcgtgac acggccgcca cgaccgtgcg cacgcc
//
LOCUS       DAORPW010000320         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_27026_length_1787_cov_622.088915, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000320 DAORPW010000000
VERSION     DAORPW010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27026_length_1787_cov_622.088915"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1787
                     /locus_tag="PLB86_13830"
     CDS             <1..>1787
                     /locus_tag="PLB86_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRC66273.1"
                     /translation="DIARSAAIKHNQAAVVFSLEMSKTEITMRLLSAEAQIQLQHMRK
                     GTMREDDWQRLAKTMGRVSDAPLFIDDSPNMSLMEIRAKCRRLKQRHNLKLVVIDYLQ
                     LMSSGKRVESRQQEVSEFSRALKLLAKELEVPVIAISQLNRGPEQRTDKRPQMSDLRE
                     SGCLTADTRVLRADTGAETTLGSLYVAGARDVPVWALDDTLRYVRRPMTHVFPTGVRP
                     VFRLTLESGKEIRATANHPFLTYAGWSPLGELRTGTRVAVPRHVPAPAFFEPWEDPRV
                     ILLAQVLSDPAYTVDRKRANVIGDWLDSLGVLSTKPHKRRLPKALFHVSKPQIALFIS
                     HLWSLSGSASVAKSGDRGRIRMESPSRRLVEDVSRLLLRFGISTRLTETEPAGGARAA
                     YRLEVSGEDSQRRFLQEIGLHGESRKAAKRLLEIVRAVEEAGAGTAAVPPRVVEAMRR
                     VMSVSAAPRPVQGMGVAGVGAGAAGPHAGAASDRRAAGEPLREVAAVLDSADMDLYAV
                     NDVLWDPVVSIEADGVEEVFDATVLGGHNFVANGIAVHNSIEQDADMVILLHREEAYE
                     KETQRAGEADLIVAKHRNGPTDTIVVAFQG"
BASE COUNT          279 a          593 c          650 g          265 t
ORIGIN      
        1 gacatcgcgc ggtcggcggc gatcaagcac aaccaggccg cggtcgtgtt cagcctcgaa
       61 atgagcaaga ccgagatcac catgcgtctg ctctcggccg aggcccagat ccagctgcag
      121 cacatgcgca agggcaccat gcgcgaggac gactggcagc gcctggccaa gacgatgggg
      181 cgggtgtccg acgcgccgct gttcatcgac gacagcccca acatgtcgct catggagatc
      241 cgggccaagt gccgccggct caagcagcgc cacaacctca agctcgtcgt tatcgattat
      301 ttacaactga tgtcgtcagg aaagcgggtg gagagccgtc aacaggaggt gtccgagttc
      361 tccagggcac tcaagctgct tgcgaaggag ctcgaagtgc ctgtcatcgc catctcccag
      421 ctcaaccgag gaccggaaca gcgcaccgac aagcgcccgc agatgtccga cctgcgtgag
      481 tccggctgcc tgaccgcgga cacccgcgtg ctgcgcgccg acaccggggc ggagacgacg
      541 ctcggctccc tgtatgtcgc gggcgctcgc gacgtccccg tctgggccct ggacgacacc
      601 ctgcgttacg tccgccggcc gatgacgcac gtcttcccga cgggggtgcg gccggtcttc
      661 cggctcacct tggagtcggg caaggagatc cgcgccaccg ccaaccaccc cttcctcacg
      721 tatgccgggt ggtcacctct gggtgagctg cgcaccggca cgcgggtcgc ggttccccgg
      781 cacgtgccgg ccccggcgtt cttcgagccg tgggaggacc cgcgggtcat cctgctggcg
      841 caggtgctga gcgacccggc atacacggtc gaccgcaagc gcgcgaacgt catcggtgac
      901 tggctcgaca gcctcggggt gctgtcgacg aagccgcaca agcgccggct gcccaaggcg
      961 ctgttccacg tgagcaagcc gcagatcgcg ctgttcatct cgcacctgtg gtcgctgtcg
     1021 gggtcggcga gcgtcgccaa gtccggcgac cgcggccgga tccggatgga gtcgccgtcg
     1081 cggcggctcg tcgaggacgt gtcgcggctg ctgctgcgtt ttggcatctc cacgcgcctg
     1141 accgagaccg agccggcggg tggtgcgcgg gcggcatacc ggctggaggt gtccggcgag
     1201 gactcgcagc ggcgcttcct gcaggagatc gggctgcacg gggagtcgcg caaggcggcc
     1261 aagcggctgc tggagatcgt ccgcgccgtc gaggaggcgg gcgcggggac cgctgccgtg
     1321 ccgccgcggg tcgtcgaggc gatgcgccgg gtgatgagcg tgtccgccgc gccgcggccg
     1381 gttcagggga tgggcgtggc cggggttggt gcgggcgctg cggggccgca cgccggtgcc
     1441 gcgagtgacc ggcgggcggc gggggagccg ctgcgcgagg tcgccgcggt cctcgactcg
     1501 gccgacatgg acctgtatgc cgtcaacgac gtcctctggg acccggtcgt ctcgatcgag
     1561 gccgacgggg tcgaggaggt cttcgacgcg acggtgctgg gcgggcacaa cttcgtggcc
     1621 aacgggatcg cggtgcacaa ctcgatcgag caggacgccg acatggtgat cctgttgcac
     1681 cgggaagagg cctacgagaa ggaaactcag cgggcgggtg aggccgacct gatcgtggcc
     1741 aagcaccgta acggccccac cgacacgatc gtggtcgcct tccaggg
//
LOCUS       DAORPW010000321         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_27138_length_1783_cov_659.976852, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000321 DAORPW010000000
VERSION     DAORPW010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27138_length_1783_cov_659.976852"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLB86_13835"
     CDS             <1..468
                     /locus_tag="PLB86_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRC66274.1"
                     /translation="HQGTQLIPADFIAVATPAYPLTDGAADVHELFLANFFAQTAALA
                     FGKTADEVRAEGTAEAIVPARVFSGNRPTTSIMAPALTPSVVGQLVALYEHITFTQGI
                     VWGIDSFDQWGVELGKQLAKKIEPAVAGDEVALGDQDASTQSLIRYYLAHRER"
     gene            complement(494..1765)
                     /locus_tag="PLB86_13840"
     CDS             complement(494..1765)
                     /locus_tag="PLB86_13840"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66275.1"
                     /translation="MKASEAAEVGRVVGEALLGTARRSGQLSAAILDRGYGAAARIAG
                     PAHRDRIERVRTAHRQATTRAGQAVGRGLGAIGHGVARVGARFADGRPVVEEPGAYQA
                     VSVLNGLYGDRLQQRHSPLSLPMALRAEGRDVPLSRGGLHAAYPHATGLVVVFIHGLV
                     ETDDWWDIRSERNWGHPGESYSRRLRAEGAWTPVRVRYNTGLRVSNNGQHLDDLLDAL
                     VAGWPVPVCGLVLVGHSMGGLVLHSALAQASSDRQWPSLVRVTVTIGTPHQGAAIVRG
                     VARLTPVLARLPETRWLAEVISSRPAGMGDLGHGNLLADDWHDVDSGALTAPARTVPL
                     AGGIRHCVVIGTVPSKRDGLVAGWVGDLLVRPADAQVAASRGEVDPRDIRVVGGARHL
                     DLLNHPAVYVHLSEWLAFAARHSPAPEPSEG"
BASE COUNT          268 a          649 c          620 g          246 t
ORIGIN      
        1 caccagggca cccagctcat cccggcggac ttcatcgccg tggccacacc ggcatacccc
       61 ttgacggacg gtgccgccga cgtgcacgag ctgttcctcg cgaacttctt cgcgcagacc
      121 gcggcgctgg ccttcggcaa gaccgccgac gaggtgcgcg cggaggggac ggccgaggcg
      181 atcgtgcccg cgcgggtctt ctcgggcaac cgcccgacga cctcgatcat ggcgcctgcg
      241 ttgaccccgt cggtggtcgg tcagctcgtc gcgctctatg agcacatcac cttcacgcag
      301 ggcatcgtct ggggcatcga ctccttcgac cagtggggtg tcgagctcgg caagcaactg
      361 gccaagaaga tcgagcctgc ggtggctggc gacgaggtcg cgctcggcga ccaggacgcc
      421 tccacccaat cgctcatccg ctactacctg gctcaccggg agcgctgaac gatcgatcag
      481 cccggcagcg gagtcagccc tcggacggct ccggtgccgg gctgtgtcgt gcggcgaagg
      541 cgagccactc gctgaggtgg acatagaccg cagggtggtt gagcaggtcg aggtggcggg
      601 cgccgcccac aacacggatg tccctcgggt cgacctcgcc ccggctagcc gcgacctgcg
      661 cgtcggcggg acgaaccaac aggtcgccca cccaacccgc cacgagccca tcgcgtttcg
      721 acggcaccgt gccgataacg acgcagtggc gtatgccgcc cgccagcggc accgttcgcg
      781 ccggcgcggt gagcgcgccg gagtcgacgt cgtgccagtc gtcggcgagc aggttcccgt
      841 gcccgaggtc acccatcccc gccgggcggg acgagatcac ctcggccagc catcgggtct
      901 ccgggagtct ggcaaggact ggggtgagcc gggcgacgcc acggacgatg gcggccccct
      961 ggtgcggagt tccgatggtg accgtgaccc ggaccagact cggccactgg cggtcggacg
     1021 aggcctgggc gagggcgctg tgcaggacca acccgcccat cgagtggccg acgaggacca
     1081 gcccgcacac ggggaccggc catcccgcga cgagcgcgtc gagcaggtca tcgaggtgct
     1141 ggccgttgtt cgacacccgc agcccggtgt tgtagcgcac ccgcaccggc gtccacgcgc
     1201 cctcggcccg cagtcgacgc gagtagctct cgcccggatg cccccagttg cgttcggagc
     1261 ggatgtccca ccagtcgtcg gtctcgacga gcccgtggat gaagaccaca acgagcccgg
     1321 tcgcgtgcgg gtatgccgcg tgtagacctc cgcgcgagag gggcacgtcg cgcccctcgg
     1381 ctcgcagggc catcggcagg ctgagcggcg agtgtcgttg ctgaagtcgg tcgccgtaga
     1441 ggccgttgag cacggacacc gcctgatagg cgccgggctc ctcgacgacc ggccgaccgt
     1501 cggcgaaacg tgccccgacc cgggccacac catgtccgat ggcgcccaga ccgcgtccca
     1561 cggcctgccc ggcccgggtg gtcgcctggc gatgcgcggt gcggacccgc tcgatccggt
     1621 cgcggtgggc cggcccggcg atgcgcgccg cggcgccgta gccacgatcg aggatcgccg
     1681 cggacagttg gccgctgcgt ctcgcggtgc cgaggagggc ctcccccacc acccgaccga
     1741 cctctgcagc ctccgacgcc ttcattactc aagagtaact tgc
//
LOCUS       DAORPW010000322         1774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_27376_length_1774_cov_736.155323, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000322 DAORPW010000000
VERSION     DAORPW010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27376_length_1774_cov_736.155323"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..908
                     /locus_tag="PLB86_13845"
     CDS             45..908
                     /locus_tag="PLB86_13845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC66276.1"
                     /translation="MTSNLPMSGQTALVTGATGGIGLATAVALAGLGARVGVVGRDEK
                     RTAAAVQAVLAVPGSGGADGFLADFSDQREVRVLAAEVLAAYPRLDVLVNNAGGYWSD
                     RHVSADGLEWTFAVNHLAPFLLTSLLEERLRESAPARIVTVASDAHRAGRIEFDDLQA
                     ERGYAGMRAYAQSKLANILFTRELARRLSGSGVAAYSVHPGTVRTGFAREDAGRLFGL
                     VLRAGALLMKSPEQGAQTSVYAASSPELVGRTGEYLAKSRVAKPSARAQDDVAAARLW
                     SVSEDLVAGSA"
     gene            978..>1774
                     /locus_tag="PLB86_13850"
     CDS             978..>1774
                     /locus_tag="PLB86_13850"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRC66277.1"
                     /translation="MPEVFDCSAADGLALGVAAAAQAVRDGQVVVLPTDTVYGIGCDA
                     FDASAVAAVLAAKGRGREMPPPVLVPGVRTIDGLAREVPDYARALIDRFWPGALTLVL
                     RAQSSLQWDLGDTNGTVAVRMPDDPVALALLNEIGPMAVTSANRTGMPPATTAAEAQS
                     QLGESVTVYLDGGPSSGGPASTILDCTGAEPIVLREGAVAEAEIREVVAAIRAAADQA
                     AAEAAASVALEQAKRRKITEQEQAERDRAADPRRRPGSAKKVAGSRRR"
BASE COUNT          228 a          596 c          675 g          275 t
ORIGIN      
        1 ggcgccgttc gacagaccga tccgggccgt gatgtgctcc gcccatgacc tcaaacctgc
       61 cgatgtccgg tcagaccgcg ctcgtgaccg gtgcaacggg cgggatcggc ctggcgacgg
      121 cggtggcgct cgccggtctc ggggctcgcg tcggcgtcgt cggacgcgat gagaagcgca
      181 ctgccgcagc ggtgcaggcg gtgttggcgg tgcccgggtc gggcggtgcc gacgggtttc
      241 tcgcggactt ctccgaccag cgcgaggtgc gggtgcttgc ggcggaagtg ctcgcggcat
      301 acccccgcct ggacgtgctg gtcaacaatg ccggcggcta ctggagcgat cggcacgtga
      361 gcgccgacgg tctggagtgg accttcgcgg tcaatcacct cgccccgttc ctgctgacca
      421 gcctgctcga ggagcgtctg cgcgagagcg cgcccgcgcg gatcgtcacc gtcgcctcgg
      481 atgcccatcg ggccgggcgg atcgagttcg acgacctgca ggcggagcgc ggttacgcgg
      541 gcatgcgggc ttatgcccag tccaaactgg ccaacatctt gttcacccgc gagttggcgc
      601 ggcggctgtc cggctccggc gtggccgcct actcggtgca cccgggcacg gtgcggaccg
      661 ggttcgctcg ggaggacgcc ggtcgcctct tcggcctggt cctgcgagcg ggcgcgttgc
      721 tgatgaagtc ccccgagcag ggggcccaaa cctcggtgta tgccgcgtcg tcgcccgagt
      781 tggtagggcg cacgggggag tacctggcca agtcacgggt cgccaagccg agtgcgcgtg
      841 ctcaggacga cgttgccgcc gcccggctct ggtcggtgag cgaggacctc gtggcggggt
      901 cagcctgagg gtgcgccggg gttcgcgcgc tggttgacga acgcctaacg gtcgccggtc
      961 gtcgtaaggt gggcgccatg cccgaggtgt tcgactgctc tgccgccgat ggtctggcgc
     1021 tcggtgttgc ggcagcagcc caggcagtcc gggatggtca ggtcgtggtg ttgccgaccg
     1081 acacggtcta tgggatcgga tgcgacgcct tcgacgcgtc ggctgtcgca gcggtgctcg
     1141 ccgccaaagg tcgcggacgc gagatgcccc cgccggtgct cgtgcccggt gtgcgcacga
     1201 tcgacgggct ggctcgcgag gtgccggact acgcgcgcgc gctcatcgac cggttctggc
     1261 cgggggcctt gacgctggtc cttcgggcgc agtcgtcgtt gcagtgggac ctgggggaca
     1321 ccaacgggac ggttgccgtg cggatgccgg acgacccggt cgccctggcg ctgctcaacg
     1381 agatcggtcc gatggcggtc accagcgcca accgcaccgg tatgccgccc gccaccaccg
     1441 ctgccgaggc gcagtcccag ttgggggagt cggtcaccgt ctatctggac ggcggcccgt
     1501 ccagcggtgg gccggcctcg acgatcctcg actgcaccgg tgccgagccg atcgtgctgc
     1561 gcgaaggtgc cgtcgccgag gccgagatcc gcgaggtggt ggcggcgatc cgagccgccg
     1621 ctgaccaggc tgcggcagag gccgccgcga gtgtggcgct cgagcaggcc aaacggcgca
     1681 agatcaccga gcaagagcag gccgagcgcg atcgggccgc cgacccgcgt cgtcgcccag
     1741 gctcggccaa gaaggtcgcc ggctcccgcc gccg
//
LOCUS       DAORPW010000323         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_27556_length_1767_cov_821.119743, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000323 DAORPW010000000
VERSION     DAORPW010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27556_length_1767_cov_821.119743"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1417
                     /locus_tag="PLB86_13855"
     CDS             137..1417
                     /locus_tag="PLB86_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC66278.1"
                     /translation="MSETQRPDVIVVGGGIGGLSAALALTRKGLTVRLLERAKEFGEV
                     GAGLQIAPNCTRMLADYGLLDEAKTLGVLPQAMVMKDATDGSELTRLDLVDLERTYGF
                     PYMVIHRSDLHGIFLRACQRAGVELLTDQQCVGYENTPTGASATFADGHVEEAEVVIA
                     ADGMHSVARGLLVDDQPVNSSYVAYRGAVTIDKVMENQVSTTDVVVYIGPGCHFVQYP
                     LRGGEMFNQVAVFQSPKALAGQDDWGTPDELDKAFEGMCEPIQLGLPLMWRDRWWRMF
                     DRNPILNWVTGKIALLGDAAHPPLQYMAQGAIMAIEDGWVLAEHVGAQQAQRTADGGT
                     GVDWDRALAAYNAVRPEHCRRVLSTGRVWGELWHVDGVKREQRNIILRGRDTYDYTFT
                     DWIYGPTALTPEQEAPLYPIIPLDSVKLPEGAEV"
     gene            complement(1444..1689)
                     /locus_tag="PLB86_13860"
     CDS             complement(1444..1689)
                     /locus_tag="PLB86_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66279.1"
                     /translation="MTTTSKTACQSCGMPIESGPYCQHCVDETGALQDFETRFERMVG
                     WQQRRDPSASREALESATLDYLATMPAWREHPRVTAR"
BASE COUNT          281 a          620 c          595 g          271 t
ORIGIN      
        1 gctgcgtgca cggcatacct cgccggtcgt ggcgactcgc acctgagcgc gagccgcgac
       61 gaccacacca cccccatccc cagcctggcc gcctggcttt gacggcggag cacacggatc
      121 gacacaggag gtcatcatga gtgagaccca acgccctgac gtcatcgtcg tcggcggcgg
      181 catcggcggg ctgagcgcgg ctcttgcgct gacccgcaag ggcttgacgg ttcggctgct
      241 cgagcgggcc aaagagttcg gcgaggtggg cgccggcctg cagatcgccc ccaactgcac
      301 gcgcatgctc gccgactacg ggctgctcga cgaggccaag accctcggcg tgttgccgca
      361 ggcgatggtc atgaaggacg ccaccgacgg gtccgagttg acccggctgg acctggtcga
      421 ccttgagcgc acgtacggct tcccctacat ggtcatccac cgcagcgacc tgcatgggat
      481 cttcctgcgc gcctgccagc gggccggcgt cgagctgctc accgaccagc aatgcgtcgg
      541 ctacgagaac accccgaccg gcgcgtcggc caccttcgcc gacggacacg tcgaggaggc
      601 cgaggtggtc atcgccgccg acggcatgca ctcggtcgcc cgcggcctgc tcgtcgacga
      661 ccagccggtc aactcgtcct atgtcgccta ccgcggtgcc gtgacgatcg acaaggttat
      721 ggagaaccag gtctcgacga ccgacgtcgt cgtctacatc gggccgggct gtcacttcgt
      781 gcagtacccc ctgcgtggcg gcgagatgtt caaccaggtc gccgtcttcc agtcgcctaa
      841 agcccttgcg ggacaagacg actggggcac gccggacgag ctcgacaagg ccttcgaggg
      901 catgtgcgag ccgatccagc tcggcctgcc gctcatgtgg cgcgaccgct ggtggcggat
      961 gttcgaccgc aacccgatcc tcaactgggt caccggcaag atcgccctgc tcggcgacgc
     1021 cgcgcacccg ccgctgcaat acatggcgca gggcgcgatc atggcgatcg aagacgggtg
     1081 ggtgctcgcc gagcacgtcg gagctcagca ggcgcagcgg accgctgacg gcggcaccgg
     1141 cgtcgactgg gaccgcgcgc tcgcggcata caacgcggtc cgtcccgagc actgccggcg
     1201 ggtgctgtcc accggccggg tgtggggcga gctgtggcac gtggacggcg tcaagcgcga
     1261 gcagcgcaac atcatcctgc gtgggcgcga cacctacgac tacaccttca ccgactggat
     1321 ctatgggccg acggcgctga cgcccgagca ggaggcgccg ctctacccga tcatcccgct
     1381 cgactcggtc aagctccccg agggcgccga ggtctgatcc gccgactggc ggcgatgccg
     1441 ggcttagcgg gcggtcaccc gagggtgctc gcgccaggcc ggcatcgtcg cgaggtaatc
     1501 cagcgtcgcc gactcgagcg cctcacgaga ggcgctcggg tcgcggcgct gttgccatcc
     1561 caccatccgc tcgaaccggg tctcgaagtc ctgcaacgcc ccggtctcgt cgacgcagtg
     1621 ctggcagtag ggccccgact cgatgggcat gccgcaactc tggcaggcag tcttgctggt
     1681 ggtggtcatg aatctcgttc cctctctggt ggattcggta tgccgcgtgc tgaggcaacc
     1741 agggcggcca gggcctcgat caggccc
//
LOCUS       DAORPW010000324         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_28747_length_1725_cov_733.473653, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000324 DAORPW010000000
VERSION     DAORPW010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28747_length_1725_cov_733.473653"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..95
                     /locus_tag="PLB86_13865"
     CDS             <1..95
                     /locus_tag="PLB86_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66280.1"
                     /translation="GVPVKLVGLGEGPDDLAPFDPAAFVDALLD"
     gene            261..1688
                     /locus_tag="PLB86_13870"
     CDS             261..1688
                     /locus_tag="PLB86_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRC66281.1"
                     /translation="MSTPLMPLADATATIDYAATTFVILCASLVLLMTVPALALFYGG
                     MTRSKSVLNMMMMSFGAAGLVGVLYVLYGYSMSFGTENIAGIIANPFEKFGLAGTDGL
                     VNPFGYEGYGNIPELALVAFQLTFAVITVALISGAIADRVKFSTWMVFAGLWVTLAYF
                     PMAHMVWGGGLLSAADGGLSAAIFGSTDGKADVVPIDFAGGTVVHINAGMAGLVLALL
                     VGLRLGFGKVAMRPHNVPLTMIGAGLLWFGWFGFNAGSELAADGTAALAWVNTTAATA
                     AAMLGWLLVEKVRDGHATSVGAASGIVAGLVAITPACGALNPLGSLILGAIAGGLCAL
                     AVGLKYKFRYDDSLDVVGVHLVAGLWGTVGAGLLSTTTGLFYGGGIRQTLLQIVIALV
                     TVVFSGVVTLVLGLILKATMGWRIDKDAETSGIDQDVHAESAYDLTASSGGRFGGAFA
                     DAGITKSSTSSTPAPPASITEGASA"
BASE COUNT          238 a          608 c          561 g          318 t
ORIGIN      
        1 tgggcgtccc ggtcaagctc gtcgggctcg gcgaaggacc ggacgacttg gcgcccttcg
       61 accccgccgc gttcgtcgac gcgctgttgg actgagcgcg gcgctcgtcg cgccgcacgg
      121 tgggtggggc gcgacgcggc ataccgctgc ggtatgccgc gtcgcccccg aaacgtgatc
      181 cttacctggc gtcggcgtcg ataacacgcc cgaaacaatc ctgggcgtcc ccggaaaccc
      241 gggcgctcca tcgttaccgc atgagcacac cactgatgcc attggcagac gccacggcga
      301 ctatcgacta tgcggcgacg accttcgtca tcctgtgcgc gtcgctcgtc cttctcatga
      361 cggttcctgc gctggccctc ttctacggag gcatgacccg ctcgaaatcc gtcctcaaca
      421 tgatgatgat gtccttcgga gctgcggggc tcgtcggcgt cctctacgtc ctctacggct
      481 actcgatgtc cttcgggacc gagaacatcg ccgggatcat cgccaacccg ttcgagaagt
      541 tcgggctggc cgggaccgac gggttggtca acccgttcgg ctacgagggc tacgggaaca
      601 tccccgaact cgccctcgtc gccttccagt tgaccttcgc cgtcatcacg gtggccctca
      661 tcagcggtgc gatcgccgac cgggtgaagt tctcgacctg gatggtgttc gcgggcctgt
      721 gggtcacgct ggcctacttc ccgatggcgc acatggtctg gggcggcggc ctgctgtcgg
      781 ccgccgacgg tggcctgtcg gccgcgatct tcgggtcgac cgacggcaag gcggacgtcg
      841 tcccgatcga cttcgctggt gggacggtcg tccacatcaa cgccggtatg gccggcctgg
      901 tcctggccct cctggtcgga ctgcgcctcg gcttcggcaa ggtcgcgatg cggccgcaca
      961 acgtcccgct gacgatgatt ggcgccgggc tgctgtggtt cggctggttc gggttcaacg
     1021 ccggttcgga gttggctgcc gacggaaccg ccgccctcgc gtgggtcaac acgaccgccg
     1081 cgaccgcggc tgcgatgctc ggctggctcc tggtggagaa ggtgcgggac gggcacgcga
     1141 cgtcggtcgg cgcggcctcc gggatcgtcg ccggactcgt cgcgatcacg cccgcctgtg
     1201 gggccctcaa cccgctcggc tcgctcatcc tcggtgctat cgccggtggg ctgtgcgcgc
     1261 tggccgtggg cctgaagtac aagttccgct acgacgacag ccttgacgtc gtcggtgtcc
     1321 acctcgtggc cggcctctgg gggacggtgg gcgcgggcct gctctccacc accaccggtc
     1381 tgttctacgg tggcggtatt cgccagaccc tcctgcagat cgttatcgct ctcgtgaccg
     1441 tcgtcttctc cggagtcgtc accctcgtcc tcggtctcat cctcaaggcc acgatgggat
     1501 ggcggatcga caaggacgcc gagacctcgg gcatcgacca ggacgtgcac gccgagtccg
     1561 cctacgacct caccgccagc agcggtggcc ggttcggcgg ggccttcgcc gacgccggaa
     1621 ttaccaagtc gtccacgtcg tccacgcccg ccccgcccgc ctcgatcacg gaaggagcga
     1681 gcgcatgaag ctcgtcaccg ccatcatcaa gccccaccag ctcga
//
LOCUS       DAORPW010000325         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_30407_length_1673_cov_596.064895, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000325 DAORPW010000000
VERSION     DAORPW010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30407_length_1673_cov_596.064895"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PLB86_13875"
     CDS             complement(<1..1034)
                     /locus_tag="PLB86_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRC66282.1"
                     /translation="MTYPDDLPIAARRDDIAAAIRDHQVVVVAGETGSGKTTQLPKIL
                     LELGRGIHGMIGHTQPRRIAARAVGERLAEELGVELGGVVGYQVRFTDQSSRDSLVKV
                     MTDGILLAEMQRDRELRRYDTIIIDEAHERSLNIDFILGYLKQLLPRRPDLKVVITSA
                     TIDPGKFAQHFADAEGNPAPVIEVSGRTYPVEVRYRPLVREGLPAKNGSAGPPIEVDQ
                     VTGICEAVEELWTEASDHDEVAAHDILVFLSGEREIRDAADALAGLRLPGTEIVPLFG
                     RLSAADQHRVFARHEGRRVVLATNVAETSLTVPGIRYVVDTGTARISRYSQRTKVQRL
                     PIEPVSKASAR"
     gene            complement(1306..>1673)
                     /locus_tag="PLB86_13880"
     CDS             complement(1306..>1673)
                     /locus_tag="PLB86_13880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRC66283.1"
                     /translation="LSQLDRTRSAIIATTGKDPIPFFRFPFGDSDAHARALVAAHGYQ
                     AVGWTVDSLGWKGTSGGMTAAKVLDRVVAAARPGEIVLMHLGANPDDNTTLDADALPQ
                     IISRLRSLGYSFVTLDSLR"
BASE COUNT          240 a          559 c          612 g          262 t
ORIGIN      
        1 cgggcgctgg ccttggagac cggctcgatc ggcaaccgct gcaccttggt ccgctggctg
       61 tagcgcgaga tccgggccgt gcccgtgtcg acgacgtagc ggatgccggg gacggtcaac
      121 gaggtctcgg cgacgttggt ggccaggacc acccggcggc cctcgtggcg ggcgaagacc
      181 cgatgctggt cggctgccga gagccgcccg aagagcggga cgatctcggt gccgggaagc
      241 cgcagcccgg ccaatgcgtc tgcggcgtca cggatctcgc gttcgccgga gaggaagacg
      301 aggatgtcgt gggccgcgac ctcatcgtgg tcggaggcct cggtccacaa ctcttcgacc
      361 gcctcgcaga tgccggtcac ctgatccacc tcgatgggtg ggcccgcgga gccgttcttg
      421 gccggcagtc cctcgcgcac caacggccgg tagcgcacct cgaccggata ggtccgtccg
      481 gacacctcga tgaccggcgc cgggttgccc tcggcgtcgg cgaagtgctg ggcgaacttc
      541 cccgggtcga tggtcgccga cgtgatgacg accttgaggt ccggccgccg cggcagcagt
      601 tgcttgaggt agccgaggat gaagtcgatg ttgaggctgc gctcatgcgc ctcgtcgatg
      661 atgatcgtgt cgtagcggcg cagttcgcgg tcccgctgca tctccgccag caggatgccg
      721 tcggtcatca ccttgacgag cgagtcgcgg ctggactggt cggtgaagcg cacctgatag
      781 ccgacgactc ccccgagctc gaccccgagc tcctcggcga gccgctcccc cactgcgcgg
      841 gcggcgatcc tccgcggttg ggtgtggccg atcatgccgt ggatgccgcg cccgagttcg
      901 agaaggatct tcggtagttg ggtggtcttg cccgacccag tctcgcccgc gacgacgacg
      961 acctggtggt cgcggatcgc ggcggcgatg tcgtcgcgac gggccgcaat gggcaggtcg
     1021 tcgggatagg tgatcgtcgg gaccgacgcg cggcgccgcg cataccgctc agcccgtgcc
     1081 tcccgagact cacgcggccc tctggtcggc cgcgcctcgc gagttggctg gatctgcctc
     1141 ggctcgcccg acccgtcggt atgccgggtc gccggcttcc gacgcggccg acgccgacgg
     1201 gcgcgcggcg gctgctcggg cggtggcgtg gacacgcccg ccaggatagg ccgagacgta
     1261 tcgccgtctc gccgagattc ccggcggcgg accgacccgg acacctcagc gcagcgagtc
     1321 gagggtgacg aacgagtagc cgagggaccg caggcggctg atgatctgcg gcagagcatc
     1381 ggcatccaac gtggtgttgt cgtcagggtt ggcgcccagg tgcatgagga cgatctcacc
     1441 cggtcgggcc gctgcgacaa cgcggtcgag caccttcgca gccgtcatgc cacccgaagt
     1501 ccctttccac cccaggctgt cgacggtcca cccgacggcc tgatagccgt gggcggcgac
     1561 caaggcgcgg gcgtgggcgt cggagtcacc gaacgggaag cggaagaacg ggatcgggtc
     1621 cttgcccgtc gtcgcgatga tggccgaccg ggtgcgatcg agttggctga gca
//
LOCUS       DAORPW010000326         1668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_30563_length_1668_cov_668.406696, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000326 DAORPW010000000
VERSION     DAORPW010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30563_length_1668_cov_668.406696"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLB86_13885"
     CDS             complement(<1..887)
                     /locus_tag="PLB86_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC66284.1"
                     /translation="MRHLRARLALTTAAVVAAGLALSACGASSNPTVAASTPAGSASG
                     ATTSAVIGSADFSESQILAEVYAAALNAKGIKATTRPNIGSREVYLKAIQDGSVDIVP
                     EYTGSLLNYLDKTATAKAPDEVYAALGKAVPTGLVALDKSAAEDKNSIVVTKDTAAKW
                     SLTQIGDLVAHQAEIVVAAPPEFQTRVQGLPGLKDVYSFVPKDFRPLKGQAVVDALVN
                     GQAQAANVFSTDPSILANGFVTLADPKALFGSDNVVPLVRKDKNSAALAAALNAVSAK
                     LDTATLTKLVSQVDVDKKDA"
     gene            complement(884..1666)
                     /locus_tag="PLB86_13890"
     CDS             complement(884..1666)
                     /locus_tag="PLB86_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRC66285.1"
                     /translation="MTQIWQWLTAPEQWSGSRGIPTRLLEHLGFSALSLLLAAAIAIP
                     VGLWVGHTGRGRSVVTIANAARAVPSLGLLFAVAMFVGPRITSNLAFVIPSVVVLVLL
                     AIPPLLSGADAGVRSVDPAVRDAAYGMGMTGREVLWRVELPCALPLLLSGVRAATLQV
                     IATATIAAAVSLGGLGRYLIDGLAVSDYAQMAGGAILVAALALALDGLLALAQRLLVS
                     PGLATRPSQRRHGKFPRARGVAGGRGRVLAPAALTTHGRDLT"
BASE COUNT          261 a          537 c          639 g          231 t
ORIGIN      
        1 gcgtccttct tgtcgacgtc gacctggctc accagcttgg tgagggtggc cgtgtcgagc
       61 ttggccgaga cggcgttcag cgcggccgcc aacgcagccg agttcttgtc cttgcgcacc
      121 agcggcacga cgttgtcgga gccgaaaagc gccttcgggt cggccagcgt gacgaacccg
      181 ttggcgagga tcgacgggtc ggtggagaag acgttcgccg cctgcgcctg accgttgacc
      241 aacgcgtcga cgacggcctg gcccttgagc gggcggaagt ccttgggcac gaagctgtag
      301 acatctttga ggcccggcag gccctggacg cgggtctgga actcgggcgg cgcggccacg
      361 acgatctcgg cctggtgggc gacgaggtca ccgatctggg tgaggctcca cttggcggcg
      421 gtgtccttgg tgacgacgat cgagttcttg tcctcggcgg cggacttgtc caaggcgacg
      481 aggccggtgg ggacggcctt gccgagcgcg gcatacacct cgtcgggggc cttcgcggtg
      541 gcggtcttgt cgaggtagtt cagcagcgac ccggtgtatt cggggacgat gtcgaccgac
      601 ccgtcctgga tggccttgag atagacctcg cggctgccga tgttggggcg ggtcgtggcc
      661 ttgatgccct tggcgttgag cgccgcggca tacacctcgg cgaggatctg cgactcggag
      721 aagtcggccg acccgatgac ggccgaggtg gtggcgccgg acgccgagcc cgcgggcgtc
      781 gaggcggcga cggtggggtt ggagctggcg ccgcacgccg acagggcgag tccggcggca
      841 acaaccgctg cggtggtgag ggccaggcgg gcacgtaggt gtctcatgtc aggtctcttc
      901 cgtgagtggt cagagcagcg ggcgccagga cgcgacccct gccgcctgca acaccacggg
      961 cccgagggaa cttcccgtgg cggcgttggg acggacgcgt cgccaggccc ggcgacacga
     1021 gcagtcgctg ggcgagggcg agcaggccgt cgagggcgag ggccagcgcg gcgacgagga
     1081 tcgccccacc cgccatctgg gcatagtcac tgacggccag cccatcgatg aggtagcggc
     1141 cgagaccgcc gaggctcacc gccgccgcga tcgtcgccgt ggcgatgacc tgcagcgtcg
     1201 ccgcgcgcac cccggagagc agcaggggca gcgcacacgg cagctcgacc cgccacaaca
     1261 cctcgcggcc agtcatcccc attccgtatg ccgcgtcgcg caccgccggg tcgaccgacc
     1321 gcacgccggc gtcggcgccg gagagcaagg gcgggatggc gagcaggacg aggacgacga
     1381 cgctggggat gacgaaggcg aggttggagg tgatccgcgg gccgacgaac atcgcgacgg
     1441 cgaagagcag accgaggctg ggcaccgcgc gcgccgcgtt ggcgatggtc acgaccgatc
     1501 ggcctcggcc ggtgtgcccg acccacaagc cgacggggat ggcgatggcc gccgcgagga
     1561 gcagggacag cgccgagaac ccgaggtgtt ccaggagccg cgtcggtatg ccgcgcgacc
     1621 ccgaccactg ctcaggcgcg gtgagccact gccagatctg cgtgatca
//
LOCUS       DAORPW010000327         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_31705_length_1632_cov_579.634115, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000327 DAORPW010000000
VERSION     DAORPW010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31705_length_1632_cov_579.634115"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="PLB86_13895"
     CDS             complement(<1..1601)
                     /locus_tag="PLB86_13895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC66286.1"
                     /translation="MDTGEPAMRDAGADPGSRLGAAAGPGAGSGAAGEPQPRQPLRND
                     ATDQPALVEVASERARRAAAVTGRATARAGKAAGRATAGASKATAKGVVVGAKFMGRG
                     AKAGTERFRLFARSEGAAESGLSRVTEMQVLASAGDAAFTVALASTILALPLGQARGQ
                     VALFLVTTMAPFVVLAPLVGPLLDRWRHGRRWAIGTTLALRAFLSWVLAGVVADGSPW
                     LLPLALLCLMATRAYAVALSAGIPLVRPDAISLVTANSRQSVATLMGMLLGAAVAAPI
                     GRFGAPWSLRVAFLIYIGATVLAIRLPAAVDSQRTPPPDVATESPPAEPPSAEPPAAE
                     TPATEPSSAEGAGQGRRARARELRRLLPAAVRAALATASGAKALSGFVTLFLSFLLWD
                     QPLPGISSVLALGLVVAAAGVGNALGSFAGNRIGDRSPTVIASAGLLAAIAVGALVTA
                     YYSVPTLILLGLVSGAFGQLAKLCLDALIQDATDDETRAQVFSWSETRLQAAWVGGGA
                     LGIAMPLIARLGFGAVTAMLVGVLVA"
BASE COUNT          253 a          616 c          587 g          176 t
ORIGIN      
        1 gccacgagga cgccgaccaa catcgcggtg acggcgccga acccgagtcg ggcgatcagc
       61 ggcatggcga tccccagcgc accgccgccg acccaggccg cctgcaggcg cgtctccgac
      121 cacgagaaca cctgcgctcg cgtctcgtcg tcggtcgcgt cctggatgag ggcgtccaga
      181 cagagtttgg ccaactgccc gaatgccccg ctgaccaggc cgagcaggat cagcgtcggc
      241 accgagtagt aggccgtcac gagggcaccc acggcaatcg ccgcgaggag ccccgcggag
      301 gcgatcacgg tcggggagcg gtcgccgatc cgattgccgg cgaaggagcc cagggcgttg
      361 cccactcccg ctgccgcgac caccaggccc agggcgagca cgctcgagat gccggggagc
      421 ggctggtccc agagcaggaa cgacaggaac aacgtgacga accccgacag cgccttggcc
      481 ccagacgccg tcgcgagggc ggcgcggact gccgcaggca acaaccgccg caactcccgg
      541 gcccgggccc gccgcccttg cccggcgccc tcggccgaag agggttcggt cgcgggggtt
      601 tcggctgcag ggggttcggc cgaagggggt tcggccggag gtgactcggt cgcaacatcc
      661 ggcggcggcg tccgctggga gtcgaccgcc gccggcagcc ggatcgcaag caccgtggcg
      721 ccgatgtaga tgaggaaggc gacccgcagc gaccagggcg cgccgaaccg cccgatcggc
      781 gccgccaccg ctgcgccaag cagcatgccc atcaacgtcg cgaccgactg ccgcgagttg
      841 gctgtcacca gactgatcgc gtcgggtcgc acgaggggta tgccggccga gagggccacg
      901 gcatacgcgc gggttgccat gaggcagagc aaggcgaggg gcagcaacca gggcgacccg
      961 tccgcgacca cgccggcgag cacccacgag aggaaggccc gcagggccag cgtggtgccg
     1021 atcgcccagc gccgaccgtg ccgccaacga tccagcaggg ggccgacgag gggggcaagg
     1081 acgacgaacg gtgccatcgt cgtcacgagg aacagcgcga cctggccgcg cgcctggccg
     1141 agaggaaggg ccaggatcgt gctggccagc gccacggtga aggctgcgtc gcccgccgag
     1201 gcaaggacct gcatctcggt gacccggctc aggcccgact cagcggcgcc ttccgatcgg
     1261 gcgaacagcc ggaaccgttc cgtcccagcc ttcgcgcccc gacccatgaa cttggctccg
     1321 acgacgacac ccttggccgt ggccttgctc gccccggcgg tggctcgccc ggcggccttg
     1381 ccggcccgcg ccgtcgcccg acccgtcacc gctgccgccc gccgggcccg ttcgctcgcc
     1441 acctcgacca gcgccggttg gtcggtcgcg tcgttgcgca gcggctgtcg cggctggggt
     1501 tcccctgcag cgccagagcc cgcgccgggg ccggccgcgg cacccaagcg gctgcctgga
     1561 tcggcccccg cgtcgcgcat cgctggctcc ccggtgtcca cgcccccatc ctgccgcgtg
     1621 gacgccgacc gg
//
LOCUS       DAORPW010000328         1629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_31794_length_1629_cov_325.685515, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000328 DAORPW010000000
VERSION     DAORPW010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31794_length_1629_cov_325.685515"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLB86_13900"
     CDS             <1..554
                     /locus_tag="PLB86_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRC66287.1"
                     /translation="QGDTVTYTFKVTNTGNVTLAPVTITDTKLNLTDWACLATLAPGA
                     TALCTSTASYIIQAEDVLNKGIVNTATATGTPPTTPSGAPVIPPATDVSDTGTYPTTP
                     GHVSTVVDPATNETTIPTSITTTVTNSPTDPTSDPTVLSLAPYSLPRTGGVGVNLYTS
                     GGALIVVGGLAWFLLGLRRRRTA"
     gene            complement(678..>1629)
                     /locus_tag="PLB86_13905"
     CDS             complement(678..>1629)
                     /locus_tag="PLB86_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC66288.1"
                     /translation="RLKDSVRMPSGSLPVRGAAANNLREVDVDIPLGVLVVLTGVAGS
                     GKSSLVDGSVSGLDGVVSIDQAPIRGSRRSNPATYTGMLEPIRKAFAKANGVKPALFS
                     SNSEGACPACNGAGVIYTDLAMMAGVATTCEECEGRRFQAAVLDYRLGGLNIAEVLDL
                     PVAQAAEFFGGSSGSGQAAIPAAAAILGRLADVGLGYVRLGQPLTTLSGGERQRLKLA
                     SHMGEKGQIYVLDEPTSGLHLADVQQLLGLLDRLVDSGKSVVVVEHHQAVMAHADWII
                     DLGPGAGHDGGRIVFEGTPADLVAAKSTLTGTHLAAYIKA"
BASE COUNT          268 a          598 c          530 g          233 t
ORIGIN      
        1 gccagggcga cacggtgacc tacaccttca aggtgacgaa caccggcaac gtcactctcg
       61 cgcccgtgac gatcaccgac acgaagctga atctgaccga ctgggcctgt cttgccacgt
      121 tggctcccgg tgcaaccgcg ctctgcacct cgaccgcgag ctacatcatc caggccgagg
      181 acgtcctcaa caagggcatc gtcaacacgg cgaccgccac cggcaccccg cccacgacgc
      241 cttccggcgc cccggtgatc ccgccggcca cggacgtgtc tgacaccggg acctacccga
      301 ccacccctgg gcacgtgagc acggtggtcg acccggccac caacgagacc acgatcccga
      361 ccagcatcac gaccacggtg accaacagcc cgaccgatcc gacgagcgac cccacggtgc
      421 tctcgttggc gccctactcg ctgccgcgca ccggtggcgt cggggtgaac ctctacacct
      481 cgggtggcgc gttgatcgtc gtgggcggat tggcctggtt cctgctgggc ctgcgtcgcc
      541 gccgtaccgc ctgaccggac aacgcgctcg gacgccggcg cgcggcatac cagggagagc
      601 gacaaggtat gccgcgcgtc ggtccgtttc tgcgggcact tcgcccgctc tgccgcgtgg
      661 ggagggcggg ctcgcagtta ggccttgatg tatgccgcga ggtgcgttcc cgtgagggtt
      721 gacttcgcgg cgaccaggtc ggcaggcgtg ccctcgaaga cgatccggcc cccgtcatgt
      781 cccgcgccgg gcccgaggtc gatgatccag tcggcgtgcg ccatgaccgc ctggtggtgc
      841 tcgacgacaa cgactgactt gccggagtcg acgagccggt cgagcagacc gagcaactgc
      901 tggacatcgg ccaggtgcag accagatgtc ggctcgtcga gcacgtaaat ctggcccttc
      961 tcgcccatgt ggctggcgag cttgaggcgc tgacgctcgc cgcccgagag cgtggtgagg
     1021 ggctggccga gccggacata gcccaacccg acgtcggcca accggccgag gatcgccgcg
     1081 gcggccggga tcgccgcctg cccggagccg gagctgcctc cgaagaactc ggccgcctgg
     1141 gcaaccggca gatcgagaac ctcggcgatg ttgagcccac cgagccggta gtccagcacc
     1201 gccgcctgga accgccgccc ctcgcactcc tcacaggtcg tcgccacccc tgccatcatc
     1261 gccaggtcgg tgtagatgac cccggcgccg ttgcaggccg ggcaggcgcc ctccgagttc
     1321 gagctgaaca gagccggctt cacgccgttc gccttggcga aagccttgcg gatcggctcg
     1381 agcatccccg tataggtcgc cgggttgctg cgccgagacc cacggatggg tgcctggtcg
     1441 atcgatacca ccccgtcgag cccggacacc gagccgtcga cgagcgagct cttgcccgac
     1501 ccggccacac cggtcaggac gacgagcacc cccaacggga tgtcgacatc gacctcgcgc
     1561 aggttgttgg ccgcggcgcc gcgcacggga agcgagccgg acggcatacg caccgagtct
     1621 ttgagccgc
//
LOCUS       DAORPW010000329         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_32801_length_1601_cov_838.259379, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000329 DAORPW010000000
VERSION     DAORPW010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32801_length_1601_cov_838.259379"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLB86_13910"
     CDS             <1..431
                     /locus_tag="PLB86_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRC66289.1"
                     /translation="PLPSPDDCVPWRPSEIWPGGYIASIELRHTPDKQPGTGRAWIRT
                     DKAVVADEEVAPIAAYLGLVDTANGMNVRVDPSEWLFPNIDLTVHLHRDPLGGPGHWV
                     GFDTQVTFGREGVGLTSTTLHDESGAVGRAEQILTLRAGR"
     gene            445..1389
                     /locus_tag="PLB86_13915"
     CDS             445..1389
                     /locus_tag="PLB86_13915"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRC66290.1"
                     /translation="MPSAPPHPLLPWIEGYAVRLGSPLTSPTAWVTTASGAELAFVGG
                     LVVKLHHPRTDVAELRTRLALATDPALAAYLVPPASSGVAVAPDGRAVTAWPRVEVLD
                     PADADREGATVPWADAGRLLAGLHRARPLGSMPVHGGWARLRRAVVRAEGGADVDPRL
                     GMVAAAGQRVLAGLDLAAADPASRPDRPLTVVHGDWHLGQLGRVDGAWRLLDLDDLGW
                     GDPAWDLARPAGFWAAGLLPTGDWEGFLAAYRAAGGPGVPVTGDPWPALDLPARAAVV
                     IAATRAVGAVGAVGARRSGADTHSDHTAEALLDACRKM"
     gene            1399..>1601
                     /locus_tag="PLB86_13920"
     CDS             1399..>1601
                     /locus_tag="PLB86_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HRC66291.1"
                     /translation="MSQIPPFPQDSATPAQQPSALVCPKCRGTMRTYDRNGIHVEQCE
                     TCRGLFLDFGEFEALARLEANVHA"
BASE COUNT          208 a          583 c          582 g          228 t
ORIGIN      
        1 cacccctgcc cagccccgac gactgtgtcc cgtggcggcc gtcggagatc tggcccggcg
       61 gctacatcgc gagcatcgag ttgcggcata cccctgacaa gcaacccggc accgggcgag
      121 cctggatccg caccgacaaa gccgtggtcg cggacgagga ggtggcgccg atcgcggcat
      181 acctcgggct cgtcgacacc gccaacggga tgaacgtccg cgtcgacccg agcgagtggc
      241 tcttccccaa catcgacctc accgtccacc tccaccggga cccgctcggc ggcccggggc
      301 actgggtggg cttcgacacg caggtgacct tcgggcgcga gggcgtcggc ctcacctcga
      361 cgacgctgca cgacgagagc ggcgcggtcg gtcgagccga gcagatcctc accctccgcg
      421 ccgggcgctg accagccagc cggtatgccg tccgcgcccc cgcatccgct cctcccctgg
      481 atcgaggggt atgccgtccg cctcggttcc cctctcacct caccgaccgc ttgggtcacc
      541 accgcaagcg gcgccgaatt ggccttcgtc ggcggcctgg tcgtcaagct gcaccacccc
      601 cgcaccgatg tcgccgagct gcggacgcga ctggcgctgg ccactgatcc ggcgcttgcg
      661 gcatacctcg tgccgccggc gagcagcggg gtcgcggtgg cgccggacgg tcgagcggtg
      721 accgcgtggc ctcgggtaga ggtcctcgac cccgccgacg ccgaccgtga aggggccacg
      781 gtgccctggg ccgatgccgg ccggctgctt gccgggctgc accgggccag gccgttgggg
      841 tcgatgccgg tgcatggggg ctgggcgcga ctacggcgcg cggtcgtgcg cgccgagggc
      901 ggggctgacg tcgacccgag gctggggatg gtcgcggcgg ccggtcagcg cgtcctcgcc
      961 ggtcttgatc tggctgcagc cgaccctgcc tcgcggcccg accggcccct cactgtggtc
     1021 catggcgact ggcatctggg ccaactgggg cgcgtcgacg gggcgtggcg gctgctggac
     1081 ctcgacgatc tcgggtgggg cgacccggcg tgggacctgg cccgaccggc cggattctgg
     1141 gctgctgggc tactgccgac gggcgattgg gagggtttcc tcgcggcata ccgtgctgcc
     1201 ggcggtcccg gcgtgccggt gacgggcgac ccgtggccgg cgctggatct gccggcgcga
     1261 gcggcagtcg tcatcgcggc gacccgcgcg gtcggcgctg tcggggctgt cggcgcgagg
     1321 cggtcgggag ctgacacgca cagcgaccac acagcggagg cactgctgga tgcctgccgg
     1381 aagatgtgaa ctctcgccat gagccagatc ccaccgttcc cgcaggactc cgccaccccc
     1441 gcccagcagc cgagcgccct cgtgtgcccc aagtgtcgcg ggacgatgcg gacctacgac
     1501 cgcaacggaa ttcacgtcga gcagtgcgag acctgtcggg gcttgttcct cgacttcggc
     1561 gagttcgagg cgctcgcgcg gctggaagcc aacgtgcacg c
//
LOCUS       DAORPW010000330         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_33394_length_1585_cov_880.802614, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000330 DAORPW010000000
VERSION     DAORPW010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33394_length_1585_cov_880.802614"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1502
                     /gene="serC"
                     /locus_tag="PLB86_13925"
     CDS             372..1502
                     /gene="serC"
                     /locus_tag="PLB86_13925"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HRC66292.1"
                     /translation="MTDQPATLTIPTDLLPGDGRFGCGPSKVRPEQVARLSEIGGTIL
                     GTSHRQAPVKGLVGEIRSGLRDLFSLPEGYEIILGNGGSTAFWDIAAFGLVRAKAQHL
                     SFGEFSNKFASVTNTAPFLGESTIIKAAPGTLATAVAEAGADVYAWPHNETSTGVMAP
                     VQRVEGADADALVLIDATSGAGGLPVDMSQADVYYFAPQKCFASDGGLWLAAFSPAAL
                     ARVEEITASGRWVPDFYSLPTAIDNSLKNQTYNTPAVATLAMFAAQLDWMNGNGGLAF
                     TTGRTADSASRLYGWAERTAYTTPFVSDPGARSQVVGTIDFDDTVDAAAVAKVLRANG
                     IVDVEPYRKLGRNQLRIAMFPAIDPEDISALTASIDWVVGQL"
BASE COUNT          231 a          611 c          504 g          239 t
ORIGIN      
        1 gaaacccatc ccgaagtctg ggccgcggcg ccgtcccggg ccgcgccggc ggccctcggt
       61 gggctcggcg tcggtggggt ggtcgccgtc tgctgacgaa gagtgatgat gcgggtgcat
      121 ggtgattcct ctcttgcact gattacaacg ttacaggtgc agaacgtgtg cgcctcgaca
      181 gttgttccca ggcgccccac ccatgaagtg acacgcgcca catcgagccc ggctcccgcc
      241 tgcgcgcgcg gggccacgcg tccacgacct ggacccgctg gcaacgccgc cgcgccgggt
      301 tgggacactt gacgtgcccc gcgccggccc ggcgcccggc acgacgaccc gccatcgaag
      361 gttgaacgat cgtgaccgac cagcccgcca ccctcaccat ccccaccgat ctgctccccg
      421 gcgacggccg cttcggctgc ggccccagca aggtccgccc cgagcaggtg gcccgcctca
      481 gtgagatcgg cggcacgatc ctcggcacgt cccaccgcca ggcgccggtc aagggcctgg
      541 tcggtgagat ccgctccgga ctgcgcgacc tgttcagcct ccccgagggc tacgagatca
      601 tcctcggcaa cggtgggtcg acggccttct gggacatcgc cgcgttcggc ctggtgcgcg
      661 ccaaggccca gcacctgagc ttcggcgagt tctccaacaa gttcgcctcg gtgaccaaca
      721 cggctccgtt cctcggcgag tcgacgatca tcaaggccgc ccccggcacc ctggccaccg
      781 ccgtggccga ggccggcgcc gacgtctacg cctggccgca caacgagacc tcgaccggcg
      841 tcatggcccc ggtccagcgg gtcgagggcg ccgatgccga cgcgctcgtc ctcatcgacg
      901 ccacctccgg cgccggtggt ctgccggtcg acatgagcca ggccgacgtc tactacttcg
      961 ctccgcagaa gtgcttcgcc tccgacggtg gcctttggtt ggccgccttc tcccccgccg
     1021 ccctggcccg ggtcgaggag atcacggcga gcggccgctg ggtgcccgac ttctactcgc
     1081 tgcccaccgc gatcgacaac tcgctgaaga accagaccta caacaccccg gcggtcgcga
     1141 cgttggcgat gttcgcggcg cagttggact ggatgaacgg caacggcggg ctggccttca
     1201 ccaccggtcg caccgccgac tccgcctcgc gcctctacgg ctgggccgag cgcacggcat
     1261 acacgacgcc gttcgtctcc gacccgggag cccgctcgca ggtggtcggg acgatcgact
     1321 tcgacgacac ggtggacgcg gcggccgtcg caaaggtgtt gcgcgccaac gggatcgtcg
     1381 acgtggagcc ctaccgcaag ctgggccgca accagctgcg gatcgcgatg ttcccggcca
     1441 tcgaccccga ggacatctcg gccctcaccg ccagcatcga ctgggtcgtc ggccagctct
     1501 gatcgccggc tccccgtctg acccgcctga cccctcttgg cccccgctga cccctcttga
     1561 cccggagata gcgctcgcac ggtca
//
LOCUS       DAORPW010000331         1579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_33611_length_1579_cov_732.169291, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000331 DAORPW010000000
VERSION     DAORPW010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33611_length_1579_cov_732.169291"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PLB86_13930"
     CDS             complement(<1..1071)
                     /locus_tag="PLB86_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66293.1"
                     /translation="MSGQSERGLLERTTTTTPPSGPERFRPAPAPAQRAPLGVRLLSA
                     LGLLALVIGVPALLFWLSGPPPIPTRLPTREDIAGQIGIEQVVTILVAIVWLAWLQFV
                     ACVIVEAFAAVRDGGIPKALPFSGPSQRLARVLVGALLLSGVVAGQVSAVVSAVSAEN
                     LRATSSISQSVATSSAVAPTTEAPATATAATQLPSTAVGAHPDGPKVYTVAPPVGRHH
                     DSLWDIAERHLGDGRRYNEIFALNEGRAQPDGRALHLARLIQPGWQLVMPEDAIGVER
                     MHLESAPVPSSAASESAATRAVPGTERAEAGTAGDLAQGAGSVGAVSTAAASTAAAST
                     SAASSASSVDQGGTGGILDVSSP"
     gene            complement(1068..>1579)
                     /locus_tag="PLB86_13935"
     CDS             complement(1068..>1579)
                     /locus_tag="PLB86_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HRC66294.1"
                     /translation="HLPEGRVTRAARRQPAFSSQLRLRLRGRRATERGASTIFVIGMV
                     AVLFAVAGLAIDGGRVLNGKDRAYDVAEQAARAGANQLNIAAVRSAGGGQVLLDETAA
                     RNAAAAFVASVPGYSVEGIATSPTQVTVRVRGTVNSVLLSLAGFSTFTVRGDATASPV
                     TGITSGAIP"
BASE COUNT          240 a          532 c          596 g          211 t
ORIGIN      
        1 cgggctgctc acgtcgagga tgccgccggt gccaccctgg tcgaccgagg acgcgctact
       61 ggcggccgag gtgctcgcgg ccgcggtgct ggctgctgcg gtgctgacag cgccgacgga
      121 gccggcccct tgggcgaggt ctccggcggt gccggcctcg gcccgttcgg tgccggggac
      181 ggcacgcgtg gccgcagact cgctcgcagc ggacgatggc acaggagccg actccaggtg
      241 catgcgctcc acgccgatcg catcctcggg catcaccagt tgccagcccg gctggatcag
      301 ccgggccagg tgcagggccc gaccgtcggg ctgcgcgcgg ccctcgttga gggcgaagat
      361 ctcgttgtag cggcgaccgt cacccagatg gcgttcggcg atgtcccaca gggagtcgtg
      421 atggcgaccg accggtgggg cgacggtgta gaccttcggg ccgtcggggt gggcaccgac
      481 cgctgtcgag ggcagctggg tggccgcggt tgctgtcgcc ggggcttcgg tcgtgggggc
      541 aacggcgctt gaggtcgcga ccgactgcga gatcgacgag gtcgcgcgga ggttctccgc
      601 agagacggcc gagacgacgg cggacacctg tccggcgacg actcccgaca gcagcagggc
      661 cccgaccaag actcgcgcca accgctggct gggcccggag aagggcaacg ccttgggtat
      721 gccgccatcg cgcaccgccg cgaacgcctc gacgatgaca caggcgacga actgcaacca
      781 cgcgagccag acgatggcga cgaggatcgt cacgacctgc tcgatgccga tctgacccgc
      841 gatgtcctca cgggtgggca accgcgtggg gatcggcggc ggtccggaca gccagaacag
      901 cagggccggg accccgatga ccaatgcgag caggccaagc gcgctcagca accgcacgcc
      961 cagcggggcc cgctgggcgg gggcgggagc cgggcggaac cgctccggtc cgctcggtgg
     1021 cgtggtggtc gtggtccgct ccaacaggcc gcgttcgctc tgtccactca tggaatagct
     1081 cccgaggtga tgccggtgac gggtgacgcg gttgcgtctc cccgaacggt gaaggtgctg
     1141 aacccggcca ggctcaacag cacgctgttg accgtcccgc ggacgcgcac ggtcacctgg
     1201 gtcggcgagg tcgcgatccc ttcgaccgaa tagccgggga cggaggccac gaaggcggct
     1261 gcggcgttgc gagcagcggt ttcgtcgagg agcacttggc cgccgcccgc ggagcgcact
     1321 gcggcgatgt tgagctggtt ggccccggcg cgagcagcct gctcggcgac gtcgtaggcc
     1381 cggtccttgc cgttgagcac gcggccacca tcgatcgcca accccgccac ggcgaagagg
     1441 acggcaacca tgccgatgac gaagatcgtc gaggcgccgc gctcggtggc acgtcgaccg
     1501 cgcagccgca gccgcagttg agatgagaag gccggttgtc gccgggctgc gcgggtcacc
     1561 cggccctccg gaagatgtc
//
LOCUS       DAORPW010000332         1565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_34131_length_1565_cov_650.773510, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000332 DAORPW010000000
VERSION     DAORPW010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34131_length_1565_cov_650.773510"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1565
                     /locus_tag="PLB86_13940"
     CDS             <1..>1565
                     /locus_tag="PLB86_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC66295.1"
                     /translation="DEHLADPSETAGRSAILQQEIERPRVGPMRDIVATIQPEQDEIV
                     RSDLGTTVCVQGAPGTGKTAVGLHRAAWLLYAHRDRLSRAGVLVVGPNRAFLDHIGSV
                     LPALGEVEVLHTTAADLVGKAPARGLDPTETAVLKGDPRMAELLRRAVWDHVGTASEA
                     LVVPRGARKWRVAAYLVQDVLDELRERGVRYGAAGAMLPQRLAHLVLLAMERAGDSPD
                     DVVQDSVARSTAVRQYAATLWPTVDPKRVLHALWTDTELLGRHADGLLSVEEQRILLW
                     ANPPRTRSAARWSPADLVLLDELADLVDRTPSLGHVILDEAQDLSPMHLRAVGRRCST
                     GSATVLGDIAQGTTPWATASWEESLAHLGKPDALVEVLDRGFRVPAAVIAYAARLLPH
                     MAPGLGVPESVRDEPGDLVIESVEARGLAASVVAAVRAATQRPGSVGVIAADAQISAL
                     SKALSRNGIEHGTLGVDHGDVEHQIDLVPATVAKGLEFDRVVVVEPAAIADAEPDERT
                     GLRRLYVVLTRAVS"
BASE COUNT          199 a          576 c          561 g          229 t
ORIGIN      
        1 gatgagcacc tggccgaccc gagcgagacc gccggtcgca gcgcgatcct ccagcaggag
       61 atcgagcgac cccgcgtcgg ccccatgcgc gacatcgtcg cgacgatcca gcccgagcag
      121 gacgagatcg tccggtccga cctgggcacg accgtctgtg tccagggcgc cccggggacc
      181 ggcaagaccg cggtcggcct gcaccgagcg gcgtggctgc tctatgcgca ccgcgatcga
      241 ctctcccgcg cgggcgtgct cgtggtcggg cccaaccgcg cgttcctcga ccacatcggg
      301 tccgtcctgc cggcgctcgg cgaggtcgag gtgctgcaca cgacggcggc ggacctggtc
      361 ggcaaggctc cggctcgggg cctggacccg acggaaaccg ctgtgctcaa aggcgatccg
      421 cggatggctg agctgttgcg gcgggcggtg tgggatcacg tgggcaccgc ttcggaggcg
      481 ctcgtcgtcc cgcgcggggc gcgcaagtgg cgggtcgcgg catacctcgt gcaggacgtc
      541 ctcgacgagt tgcgcgagcg cggcgtgcgc tacggcgcgg cgggggcgat gctcccgcag
      601 cggttggcgc acctcgtcct gctcgccatg gagcgcgccg gcgactcccc cgacgacgtc
      661 gtccaggact ccgtcgcgcg cagcaccgcc gtccggcagt atgccgcgac gctctggccc
      721 accgtcgacc cgaagcgggt gctccacgcc ctgtggaccg acaccgagtt gctcggccgc
      781 cacgccgacg ggctcttgag cgtcgaagag cagcgaatcc tgttgtgggc caacccgcct
      841 cgcacgcgct cagccgcccg gtggtccccc gccgacctcg tccttcttga cgagttggcc
      901 gacctcgtcg accgcacccc gagcctcggc cacgtcatcc tcgacgaggc ccaggacctc
      961 tccccgatgc acctgcgcgc ggtcgggcgg cgctgctcga ccggctcggc gacggtgctc
     1021 ggcgacattg cacagggcac gacgccgtgg gcgaccgcgt cgtgggagga gtcgctggcg
     1081 cacctcggca agcccgacgc gctggtcgag gtgctcgacc gcggcttccg ggtccccgcc
     1141 gccgtcatcg cgtatgccgc ccgcctcctc ccgcacatgg cccccggtct cggggtgccc
     1201 gagtccgtcc gtgatgagcc gggtgatctc gtcatcgagt cggtcgaggc tcgggggctg
     1261 gcggcctcgg tggttgctgc cgtgcgggcc gccacgcagc ggcccgggtc ggtaggagtg
     1321 attgccgccg acgcccagat cagtgcgttg tcgaaagcgt tgtcccgcaa cgggatcgag
     1381 cacggcactc tcggagtgga ccacggcgac gtcgagcatc agatcgacct ggtgccggcc
     1441 acggtcgcta agggcctgga gtttgaccgg gtcgtcgtgg tcgagccggc cgccatcgcc
     1501 gacgccgagc ccgacgagcg caccggtctg cgccggctct atgtcgtgct gacccgcgcc
     1561 gtgtc
//
LOCUS       DAORPW010000333         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_35711_length_1525_cov_577.311565, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000333 DAORPW010000000
VERSION     DAORPW010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35711_length_1525_cov_577.311565"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLB86_13945"
     CDS             complement(<1..213)
                     /locus_tag="PLB86_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC66296.1"
                     /translation="MSVTLLKGGLLGPRDRTRRHTRVGCQRSTLAEEARNLVLAALLR
                     VLQRRHAGAIGDVPVGARRQQDLDDFL"
     gene            complement(227..526)
                     /locus_tag="PLB86_13950"
     CDS             complement(227..526)
                     /locus_tag="PLB86_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC66297.1"
                     /translation="MSSLAAALEVVGARWALLIVDRLLDGPARYGDLQRDLGVPTNIL
                     ATRLRELEAAGVLSRLPLRHSTRAYALTDRGLALREAIVALGNWGAEDPTGGSAE"
     gene            complement(523..927)
                     /locus_tag="PLB86_13955"
     CDS             complement(523..927)
                     /locus_tag="PLB86_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC66298.1"
                     /translation="MSLFITCPVESVERATAFYTALGWTLNAEMSDHNVSCFAIAPEQ
                     YVMLGSREMYASVGGVEELIGGPDTPSKVTVSFDLASREAVDEIVDRAAAAGGRVGDT
                     DDYPFMYQRQFDDPDGYHYSPFWMKPDADPTA"
     gene            complement(1014..>1525)
                     /locus_tag="PLB86_13960"
     CDS             complement(1014..>1525)
                     /locus_tag="PLB86_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRC66299.1"
                     /translation="PGATVLVQGAGGGVATALIQLGSAAGLRMWVTSRDEAKGARALE
                     IGADRAFGSGERLPDRVDAVMETVGAATWSHSVNSLKPGGAIVISGATSGDAPAKAEL
                     TKIFFKQLRVIGSTMGTKAELERLAAFVQGKGITPVIDSVRPLAEAREGFEHMANGDV
                     FGKVIFTVS"
BASE COUNT          255 a          564 c          488 g          218 t
ORIGIN      
        1 caggaagtcg tccagatcct gctggcggcg ggcgccgacc gggacatcgc cgatcgcgcc
       61 ggcgtgtcgg cgctgcagca cgcggagcaa cgcggccaga acgaggttgc gcgcctcctc
      121 cgccaacgtt gaccgctgac accccacgcg ggtatgccgt cgggtccggt cgcgcgggcc
      181 gagcaggccg cctttgagga gcgttaccga cactcccgtg tgggtatcac tccgccgagc
      241 cacccgtggg gtcctcggcg ccccaattcc ccagagcgac gatcgcctcg cgcagggcaa
      301 gcccgcgatc ggtcagcgcg taggcccggg tgctgtgccg cagtggcaac cgggacagca
      361 caccggccgc ttcgagttcg cgcagccggg tcgcgaggat gttcgtcggc acgccgaggt
      421 cgcgctgcag atcgccgtag cgcgccggcc cgtcgagcag gcgatccacg atcagcagag
      481 cccaccgtgc cccgacgacc tcgagagcgg cggcgaggct gctcacgccg tcggatcagc
      541 gtccggcttc atccagaacg gcgagtagtg atagccgtcg gggtcatcga actggcgctg
      601 atacatgaag gggtagtcgt cggtgtcacc gacgcgccca ccagcggcag cggcccggtc
      661 gacaatctcg tcgaccgcct cgcggctggc caggtcgaag gagaccgtga ccttcgaggg
      721 cgtgtccggc ccaccgatca actcctcgac cccgccgacg ctcgcataca tctcgcggct
      781 gccgagcatg acgtactgct cgggcgcgat cgcgaagcac gacacgttgt gatcggacat
      841 ctcggcgttg agggtccacc cgagggcggt atagaaggca gtcgcgcgct cgacgctctc
      901 gaccgggcag gtgatgaaga ggctcatgtg ggcgatactt gcaaattaca agtgcgatcg
      961 tcaaccggca gacgggagcc gtgaggggcc gactcccatc cgctcgctgg ctcttaggag
     1021 accgtgaaga tgaccttgcc aaagacgtca ccgttggcca tgtgctcgaa gccctcccga
     1081 gcctccgcga gcggccgcac cgagtcgatg accggcgtga tgcccttgcc ctgaacgaac
     1141 gccgccagcc gctccaactc ggccttggtc cccatcgtcg agccgatgac ccgcaactgc
     1201 ttgaagaaga tcttcgtcaa ctccgccttg gccggcgcgt cacccgaggt cgcccccgag
     1261 atgacgatcg ccccacccgg cttgagcgag ttgaccgagt gcgaccaggt cgccgccccc
     1321 accgtctcca tcaccgcatc gacccggtcc ggcagccgct cgcccgaccc gaacgcccga
     1381 tccgcgccga tctcaagcgc ccgcgcaccc ttggcctcgt cccgcgacgt cacccacatc
     1441 cgcaagccgg ccgcggaccc cagttggatc agcgccgtcg ccacgccacc gcccgcgccc
     1501 tgcacgagaa ccgttgcccc aggcg
//
LOCUS       DAORPW010000334         1519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_5385
            NODE_35948_length_1519_cov_784.765710, whole genome shotgun
            sequence.
ACCESSION   DAORPW010000334 DAORPW010000000
VERSION     DAORPW010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867602
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:31:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,794
            CDSs (total)                      :: 2,764
            Genes (coding)                    :: 2,752
            CDSs (with protein)               :: 2,752
            Genes (RNA)                       :: 30
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35948_length_1519_cov_784.765710"
                     /isolate="AMR_MDS_5385"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..645)
                     /locus_tag="PLB86_13965"
     CDS             complement(151..645)
                     /locus_tag="PLB86_13965"
                     /inference="COORDINATES: protein motif:HMM:NF022819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HRC66300.1"
                     /translation="MVTSGGVRLRQLVVVTVVVGLLGLVVLRLLGGTVPSPGWPGVVL
                     LLFMAVGVYFAALPVKRLRERRVLSPGSALRAARSLVLAQAAALTGAGLLGWYAAQVV
                     VLLPNVDVDSQRGRMWLLVAHAVAAIVLVVSGLLAQRICRIDPPDAGDPGDRATNPGG
                     VAPA"
     gene            complement(639..>1519)
                     /gene="folK"
                     /locus_tag="PLB86_13970"
     CDS             complement(639..>1519)
                     /gene="folK"
                     /locus_tag="PLB86_13970"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRC66301.1"
                     /translation="VVEGIRGLGYHGVFEHERRDGQQFLVDVAIELDLRRPGRSDNLV
                     DTVHYGEVAAQVLARIEGEPFDLIEALAEVIAADVLSLPGVEAVDVVVHKPQAPVGVP
                     VTDVRVEIRRERGTRVVIALGSNVGDRVATLTEAVEALAEVPRLALIAVSDLVESDPF
                     GGPDQPAYLNGVVLANFAGSPATLLRALHAIEHRNGRTREIRWGARTLDLDLVQFGVP
                     GSDGERLSDTPALLLPHPRAHERNFVLRPWAQLDPEATLRVGPTVAHPVRLVADVLVE
                     LGLSGVRPGPVWSPAW"
BASE COUNT          269 a          544 c          532 g          174 t
ORIGIN      
        1 cagccgacgc ggtcggtgag gcgcgctacg ccatcgacgg gatggccggc tggcgggcgc
       61 agcggcgtcg gcgccgggac cgcagacgcc tcaagggccg ccaggggatc gctgactgag
      121 ccccgacctg ccgggtcgcc tggcgggtcg ttaggcgggc gccacgcctc cagggttggt
      181 cgcccggtca ccggggtccc cggcgtcggg tgggtcgatc cgacagatcc gttgcgcgag
      241 aaggccactc accacgagca cgatcgctgc caccgcgtga gcgacgagga gccacatacg
      301 gccgcgttga gagtcgacgt ccacgttggg cagcaggacc actacctgcg cggcatacca
      361 gccgagcaag ccggccccgg tcaaagccgc cgcctgcgcc aggacgaggg atcgggcggc
      421 gcgcagggca ctccccggag agagcacgcg acgctcgcgc agccgcttga ccggcagggc
      481 ggcgaagtag acgccgacag ccatgaacag caggaggacc actcccggcc accccgggct
      541 ggggacggtg ccaccgagca gccgcaacac gaccaggccg agcaggccga cgaccaccgt
      601 gacgacgacc aactgacgca gccgcacgcc tcccgaggtc accatgccgg gctccagacc
      661 gggcccggcc ggacgccgga caggcccaac tcgacgagga cgtcagccac gaggcggacg
      721 gggtgggcaa cggtgggccc gacccgcagg gtggcctcgg ggtcgagttg cgcccacggg
      781 cgcaggacga agttgcgctc gtgcgcccga gggtgtggca gcagcagagc gggcgtgtcc
      841 gacagccgct cgccatcact ccccggcact ccgaactgaa ccagatcgag gtccaatgtc
      901 cttgctcccc aacggatctc acgcgtgcgc ccgttgcggt gctcgatcgc atgcagggcc
      961 cgcagcaggg tggcgggtga gccggcaaag ttcgcgagca ccacgccatt gaggtatgcc
     1021 ggttggtccg gtccgccgaa agggtccgac tcgacgaggt ccgacaccgc gatgagggcc
     1081 aaccgcggca cctcggcgag cgcctcgacc gcctcagtca gggtcgccac ccgatcaccc
     1141 acgttggagc ccaacgcgat gaccacccga gtgccacgct cccgacggat ctcgacccgg
     1201 acgtcggtga ccgggacccc gaccggcgcc tgcggtttgt ggacgacgac atcgacggct
     1261 tcaaccccag gaaggctcag gacatcggcg gcgatgacct cggcgagcgc ctcgatgagg
     1321 tcgaagggct ccccctcgat gcgggccaac acctgagcag caacctcgcc gtagtgaacc
     1381 gtgtcgacga ggttgtccga gcgcccaggc cgacgcaggt ccaactcgat ggcaacgtcg
     1441 acgaggaact gttgcccgtc gcggcgctcg tgctcgaaca ccccgtgata gcccaggccc
     1501 cggatgcctt cgacgacga
//
